Patent Publication Number: US-10760069-B2

Title: Producing adipic acid and related compounds using hybrid polyketide synthases

Description:
RELATED PATENT APPLICATIONS 
     The application claims priority as a continuation application to PCT International Patent Application No. PCT/US 16/41795, filed Jul. 11, 2016, which claims priority to U.S. Provisional Patent Application Ser. No. 62/191,283, filed Jul. 10, 2015; both of which are incorporated herein by reference. 
    
    
     STATEMENT OF GOVERNMENTAL SUPPORT 
     This invention was made with government support under Contract No. DE-AC02-05CH11231 awarded by the U.S. Department of Energy, and Grant Nos. EEC 0540879, DGE 1106400, and MCB 1341894 awarded by the National Science Foundation. The government has certain rights in the invention. 
    
    
     FIELD OF THE INVENTION 
     This invention relates generally to production of adipic acid and related compounds using polyketide synthases. 
     BACKGROUND OF THE INVENTION 
     Dicarboxylic acids (diacids) are important compounds that are used in the manufacture of commercial polymers (e.g. polyesters, polyurethanes). The diacid adipic acid [1] is used mainly as a monomer in the production of 6,6-nylon, a polyamide generated through the reaction of [1] with hexane-1,6-diamine. Polyesters (for use in fabrics and plastics of many compositions) are formed through the polymerization of terephthalic acid [3] and a dialcohol (diol) such as ethylene glycol (to make polyethylene terephthalate), propane diol (poly(1,3-propanediol terephthalate)) or butanediol (poly(1,4-butanediolphthalate). Adipic acid is also used in the synthesis of various polyesters. Currently adipic acid is synthesized via oxidation of cyclohexane and similar petrochemicals using traditional chemical synthesis. 
     The large scale worldwide use of nylons and polyesters requires the production of millions of metric tons of [1] and [3] annually. These diacids are themselves synthesized from starting materials extracted from petroleum. One means of reducing the large dependence on oil for the commercial production of polymers is to generate the diacids by a fermentation process involving the use of polyketide synthases. 
     The use of hybrid polyketide synthases to produce diacids with a carbon backbone with an odd number of carbon atoms is disclosed in International Patent Application No. PCT/US2009/038831, filed Mar. 30, 2009, which published as PCT publication no. WO 2009/121066 on Oct. 1, 2009. The use of hybrid polyketide synthases to produce diacids is disclosed in U.S. Patent Application Pub. No. 2013/0280766, now issued as U.S. Pat. No. 9,334,514. 
     The polyketides are one of the most diverse and chemically complicated classes of molecules known, its members frequently weighing in excess of 500 daltons and harboring numerous stereocenters. Partly owing to their antibacterial, immunosuppressive, and anti-cancer activities, much effort has been devoted to deciphering the mechanism by which polyketide synthases (PKSs) synthesize their products. PKSs perform Claisen condensation reactions between a loaded acyl-ACP intermediate and an α-substituted (H, CH 3 , C 2 H 5 , etc.) malonyl-CoA extender unit analogous to fatty acid biosynthesis. This is then followed by varying degrees of 3-reduction by accessory domains. This condensation-reduction cycle is repeated by subsequent downstream modules until the intermediate is liberated from the enzyme, most commonly by the activity of a thioesterase domain (reviewed in (Khosla, 2009)). 
     Engineering of type I modular PKSs has the potential to produce an enormous variety of novel, rationally-designed compounds. Yet, more than two decades after their modular nature was discovered (Donadio et al., 1991), there are currently no commercial applications of engineered PKSs. 
     SUMMARY OF THE INVENTION 
     The present invention provides for a polyketide synthase (PKS) capable of synthesizing a carboxylic acid, said PKS comprising a synthetic module comprising the S3c variant module, or a functional variant thereof, wherein the PKS is capable of synthesizing a carboxylic acid. 
     The present invention also provides for a polyketide synthase (PKS) capable of synthesizing a carboxylic acid, said PKS comprising a hybrid module comprising a BorA2 KS domain, or functional variant thereof, a BorA2 AT domain, or functional variant thereof, a DH described in Example 1, or functional variant thereof, a heterologous KR domain, a heterologous ER domain, and a BorA2 ACP domain, or functional variant thereof, wherein the PKS is capable of synthesizing a carboxylic acid. 
     The present invention provides for a recombinant nucleic acid that encodes a polyketide synthase (PKS) of the present invention. The recombinant nucleic acid can be replicon capable of stable maintenance in a host cell. In some embodiments, the replicon is stably integrated into a chromosome of the host cell. In some embodiments, the replicon is a plasmid. The present invention also provides for a vector or expression vector comprising a recombinant nucleic acid of the present invention. The present invention provides for a host cell comprising any of the recombinant nucleic acid and/or PKS of the present invention. In some embodiments, the host cell, when cultured under a suitable condition, is capable of producing the carboxylic acid or diacid. 
     The present invention provides for a host cell comprising any of the recombinant nucleic acid and/or PKS of the present invention. In some embodiments, the host cell, when cultured, is capable of producing a carboxylic acid or diacid. 
     The present invention provides a method of producing a carboxylic acid or diacid, comprising: providing a host cell of the present invention, and culturing said host cell in a suitable culture medium such that the carboxylic acid or diacid is produced. 
     The present invention provides for a composition comprising a carboxylic acid or diacid isolated from a host cell from which the carboxylic acid or diacid was produced, and trace residues and/or contaminants of the host cell. Such trace residues and/or contaminants include cellular material produced by the lysis of the host cell. In some embodiments, the trace residues and/or contaminants do not or essentially do not interfere or retard a polymerization reaction involving the carboxylic acid or diacid. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The foregoing aspects and others will be readily appreciated by the skilled artisan from the following description of illustrative embodiments when read in conjunction with the accompanying drawings. 
         FIG. 1A  shows loops were introduced combinatorially into BorMod1 using two alternative N-terminal and a single C-terminal splice sites to generate eight chimeras to be tested for adipoyl-ACP production. Abbreviations: KS: ketosynthase domain; AT: acyltransferase domain; KR: ketoreductase domain; DH: dehydratase domain; ER: enoylreductase domain; ACP: acyl carrier protein domain. 
         FIG. 1B  shows the loading and first extension modules of the borrelidin PKS (hereafter referred to as “BorLM” and “BorMod1”, respectively) are capable of producing a 3-hydroxy-adipoyl-ACP intermediate in vitro using succinyl-CoA as a starter substrate and the natural extender substrate, malonyl-CoA. Abbreviations: KS: ketosynthase domain; AT: acyltransferase domain; KR: ketoreductase domain; DH: dehydratase domain; ER: enoylreductase domain; ACP: acyl carrier protein domain. 
         FIG. 2A  shows extension intermediate analysis of BorMod1 variants in an initial library. Variants designated by reductive loop source (A=AurB, I=IdmO, N=NanA2, S=SpnB); N-terminal junction (1, 2, 3) and BorDH2 presence (null=wildtype DH domain, t=in trans, c=in cis). 
         FIG. 2B  shows the effect of BorDH2 in trans (indicated using t). Variants designated by reductive loop source (A=AurB, I=IdmO, N=NanA2, S=SpnB); N-terminal junction (1, 2, 3) and BorDH2 presence (null=wildtype DH domain, t=in trans, c=in cis). 
         FIG. 2C  shows the effect of BorDH2 in cis (indicated using c). Variants designated by reductive loop source (A=AurB, I=IdmO, N=NanA2, S=SpnB); N-terminal junction (1, 2, 3) and BorDH2 presence (null=wildtype DH domain, t=in trans, c=in cis). 
         FIG. 2D  shows the effect of junction 3 without and with BorDH2 in cis. Variants designated by reductive loop source (A=AurB, I=IdmO, N=NanA2, S=SpnB); N-terminal junction (1, 2, 3) and BorDH2 presence (null=wildtype DH domain, t=in trans, c=in cis). 
         FIG. 3  shows the descarboxy substrates propionyl- and CPMA-ACP were extended and fully reduced to their respective products by both S3 and S3c protein variants. Abbreviations: KS: ketosynthase domain; AT: acyltransferase domain; KR: ketoreductase domain; DH: dehydratase domain; ER: enoylreductase domain; ACP: acyl carrier protein domain; n.d.: not detected; mins: minutes. 
         FIG. 4  shows the BorMod1-TE construct produced exclusively 3-hydroxy-adipic acid whereas S3c-TE produced a mixture of the partially and fully reduced adipic acid products. Abbreviations: KS: ketosynthase domain; AT: acyltransferase domain; KR: ketoreductase domain; DH: dehydratase domain; ER: enoylreductase domain; ACP: acyl carrier protein domain; TE: thioesterase. 
         FIG. 5 . LC-MS/MS chromatograms of extension reactions using different starter substrates (CPMA-, CPDA-ACP) and BorMod1 variants (S3, S3c). Identity of each peak indicated by molecule appearing above it (3-cyclopentyl-3-hydroxypropanoyl intermediate was not detected; small peak at RT approximately 9.5 mins is a contaminant found in all samples), Abbreviations: KS: ketosynthase domain; AT: acyltransferase domain; KR: ketoreductase domain; DH: dehydratase domain; ER: enoylreductase domain; ACP: acyl carrier protein domain; n.d.: not detected; mins: minutes. 
         FIG. 6 . N- and C-terminal junctions for initial constructs. Arrows indicates crossover point. The amino acid sequence of  Streptomyces parvulus  BorA2 is SEQ ID NO:6. The amino acid sequence of  Streptomyces thioluteus  AurB is SEQ ID NO:7. The amino acid sequence of  Streptomyces antibioticus  IdmO is SEQ ID NO: 8. The amino acid sequence of  Streptomyces nanchangensis  NanA2 is SEQ ID NO:9. The amino acid sequence of  Saccharopolyspora spinosa  SpnB is SEQ ID NO:10. 
         FIG. 7 . Junctions for DH swap constructs. Arrows indicates crossover point. The amino acid sequence of  Streptomyces parvulus  BorA2 is SEQ ID NO:6. The amino acid sequence of  Streptomyces thioluteus  AurB is SEQ ID NO:7. The amino acid sequence of  Streptomyces antibioticus  IdmO is SEQ ID NO: 8. The amino acid sequence of  Saccharopolyspora spinosa  SpnB is SEQ ID NO:10. 
         FIG. 8 . N-terminal junctions including junction 3. Arrows and lines indicate crossover points. The amino acid sequence of  Streptomyces parvulus  BorA2 is SEQ ID NO:6. The amino acid sequence of  Streptomyces thioluteus  AurB is SEQ ID NO:7. The amino acid sequence of  Saccharopolyspora spinosa  SpnB is SEQ ID NO:10. 
         FIG. 9  shows a scheme for making novel polyamides or novel polyesters using diacids (using adipic acid as an example). 
         FIG. 10  shows different domains for terminating the synthesis of the compound. Abbreviations: KS: ketosynthase domain; AT: acyltransferase domain; KR: ketoreductase domain; DH: dehydratase domain; ER: enoylreductase domain; ACP: acyl carrier protein domain; TE: thioesterase. 
         FIG. 11  shows different sides chains that can be added using different extenders. 
     
    
    
     DETAILED DESCRIPTION 
     Before the present invention is described, it is to be understood that this invention is not limited to particular embodiments described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims. 
     Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limits of that range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in that stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range, and each range where either, neither or both limits are included in the smaller ranges is also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention. 
     Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are now described. All publications mentioned herein are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the publications are cited. 
     It must be noted that as used herein and in the appended claims, the singular forms “a”, “and”, and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a diacid” includes a plurality of such diacids, and so forth. 
     The term “functional variant” describes an enzyme that has a polypeptide sequence that is at least 70%, 75%, 80%, 85%, 90%, 95% or 99% identical to any one of the enzymes described herein. The “functional variant” enzyme may retain amino acids residues that are recognized as conserved for the enzyme, and may have non-conserved amino acid residues substituted or found to be of a different amino acid, or amino acid(s) inserted or deleted, but which does not affect or has insignificant effect its enzymatic activity as compared to the enzyme described herein. The “functional variant” enzyme has an enzymatic activity that is identical or essentially identical to the enzymatic activity of the enzyme described herein. The “functional variant” enzyme may be found in nature or be an engineered mutant thereof. 
     These and other objects, advantages, and features of the invention will become apparent to those persons skilled in the art upon reading the details of the invention as more fully described below. 
     This invention provides for an engineered enzyme capable of production of hexane 1,6-dicarboxylic acid (“adipic acid”) when used in conjunction with its native loading module or provided a synthetic substrate. Successful production of adipic acid comprises one or more of the following: (1) selection of suitable reductive loop donors, (2) elucidating chimeric junction boundaries that result in high enzyme activity, (3) replacement of the dehydratase domain in the reductive loop, and (4) concatenation of a thioesterase domain. 
     In some embodiments, the enzyme is loaded with a succinic acid analog (presented as succinyl-acyl carrier protein (“succinyl-ACP”) from the upstream loading module or as a synthetic succinyl-n-acetyl-cysteamine (“succinyl-SNAC”), which is then condensed with malonyl-coenzyme A (“malonyl-CoA”) to produce 3-keto-adipic-acyl carrier protein (“3-keto-adipic-ACP”). The engineered “reductive loop” processively reduces this intermediate with NADPH to adipic-acyl-carrier protein (“adipic-ACP”), which is hydrolytically released from the enzyme by the action of the thioesterase (“TE”) domain. An aspect of the invention is the replacement of the dehydratase domain in the reductive loop. 
     Polyketide Synthases (PKS) 
     In some embodiments, the synthetic module comprises one or more of the following domains: a BorA2 KS domain, or functional variant thereof, a BorA2 AT domain, or functional variant thereof, a DH described in Example 1, or functional variant thereof, a heterologous KR domain, a heterologous ER domain, and a BorA2 ACP domain, or functional variant thereof. 
     In some embodiments, the heterologous KR domain is a KR domain of AurB, IdmO, NanA2, or SpnB, or a functional variant thereof. In some embodiments, the heterologous ER domain is an ER domain of AurB, IdmO, NanA2, or SpnB, or a functional variant thereof. 
     In some embodiments, the PKS further comprises a second module comprising a BorA1 AT domain, or a functional variant thereof, and a BorA1 ACP domain, or a functional variant thereof. 
     In some embodiments, the PKS further comprises one or more extender modules or domains, and a thiosterase (TE) domain, such as ery TE, or an R domain. In some embodiments, the PKS is modified as shown in  FIG. 10  wherein the PKS has the domains KR-ACP-ST-TE. 
     In some embodiments, the PKS further comprises one or more extender modules or domains between the synthetic module or hybrid module, and TE domain or R domain. 
     The amino acid sequence of the S3c variant is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 1) 
               
            
           
           
               
            
               
                 MAHEDKLRHLLKRVSAELDDTQRRVREMEESEREPIAIVGMSCRLPGGVN 
               
               
                   
               
               
                 SPGEFWSLLEAGTDAVSEFPRDRGWDVENLYDPDPDAPGRSYVREGGFLD 
               
               
                   
               
               
                 GAGQFDAAFFGISPREALAMDPQQRLLLECSWEAIERSRIDPKTLHGSRT 
               
               
                   
               
               
                 GVFAGSNWQDYNTLLLNAEERSQSYLATGASGSVLSGRVSYTLGMEGPAI 
               
               
                   
               
               
                 TVNTACSSSLVAVHLAARSLRAGECDLALAGAVTVMSTPQLPVAFSRQRG 
               
               
                   
               
               
                 LAPDGRSKAFAVSADGMGFGEGVGVLVLERLSVARRNGHRVLAVVRGSAV 
               
               
                   
               
               
                 NQDGASNGLTAPNGPSQQRVIRAALASAGLGPADVDVVEAHGTGTRLGDP 
               
               
                   
               
               
                 IEAQALLATYGRGRDAERPLWLGSVKSNIGHAQAAAGVAGVIKMVLAMEK 
               
               
                   
               
               
                 GRLPRTLHVDEPSGEVDWDSGAVRLLTEARDWPSEEGRLRRAGVSSFGIS 
               
               
                   
               
               
                 GTNAHVIIEEAPEEGEEPESDAGGVVPWVLSARTEGALQAQAVQLSEFVG 
               
               
                   
               
               
                 ESSPVDVGWSLVSTRAAFEHRAVVVGRGRDELVRGLSEVAQGRGVRGVAS 
               
               
                   
               
               
                 SASGGLAFVFAGQGSQRLGMGRGLYERFPVFAEAFDEVCGRVGPGVREVV 
               
               
                   
               
               
                 FGSDAGELDRTVWAQAGLFALEVALFRLLESWGVRPGCLIGHSVGELSAA 
               
               
                   
               
               
                 CVAGLWSLEDACRVVAARARLMQALPAGGVMVAVRAEAGELAGFLGEDVV 
               
               
                   
               
               
                 IASVNAPGQVVIAGPEGGVERVVAACGARSRRLAVSHAFHSPLVEPMLGE 
               
               
                   
               
               
                 FRRVVESVAFGVPSLRVVSNVTGAWVDPEEWGTPEYWVRQVREPVRFADG 
               
               
                   
               
               
                 VATLLDAGVRTFVELGPAGALTSMVSHCADATATSVTAVPTLRPDHDESR 
               
               
                   
               
               
                 TVLSAAASLYVQGHPVDWAPLFPRARTVDLPTYPFQHQHYWMMNTGSAAE 
               
               
                   
               
               
                 PAELGLGDARHPLLGSVVTVAGDDKVVFAGRLALRTHPWLADHTVLDAVL 
               
               
                   
               
               
                 LPATAFLELAVRAGEEVSCPVVHDLTLHRPLVVPERGAVQVQMAVGAPEA 
               
               
                   
               
               
                 DGRREVRVYSRPDDDAEHEWTLHAAGLLASAATAEPAVAAGAWPPPEAQA 
               
               
                   
               
               
                 VDLDGFYAGLAEHGYHYGPLFQGVRAAWRLGDDVLAEIVLPEAAGADAAR 
               
               
                   
               
               
                 YGMHPALLDAVLHAARLGAFRERSEEKYLPFAWEGVTLRTRGATAVRARI 
               
               
                   
               
               
                 SRAGTDAIRLDVTDTADRPVLTAESLVLRSAAARRTGARRQAHQARLYRL 
               
               
                   
               
               
                 SWPTVQLPTSAQPPSCVLLGTSEVSADIQVYPDLRSLTAALDAGAEPPGV 
               
               
                   
               
               
                 VIAPTPPGGGRTADVRETTRHALDLVQGWLSDQRLNESRLLLVTQGAVAV 
               
               
                   
               
               
                 EPGEPVTDLAQAALWGLLRSTQTEHPDRFVLVDVPEPAQLLPALPGVLAC 
               
               
                   
               
               
                 GEPQLALRRGGAHAPRLAGLGSDDVLPVPDGTGWRLEATRPGSLDGLALV 
               
               
                   
               
               
                 DEPTATAPLGDGEVRIAMRAAGVNFRDALIALGMYPGVASLGSEGAGVVV 
               
               
                   
               
               
                 ETGPGVTGLAPGDRVMGMIPKAFGPLAVADHRMVTRIPAGWSFARAASVP 
               
               
                   
               
               
                 IVFLTAYYALVDLAGLRPGESLLVHSAAGGVGMAAIQLARHLGAEVYATA 
               
               
                   
               
               
                 SEDKWQAVELSREHLASSRTCDFEQQFLGATGGRGVDVVLNSLAGEFADA 
               
               
                   
               
               
                 SLRMLPRGGRFLELGKTDVRDPVEVADAHPGVSYQAFDTVEAGPQRIGEM 
               
               
                   
               
               
                 LHELVELFEGRVLEPLPVTAWDVRQAPEALRHLSQARHVGKLVLTMPPVW 
               
               
                   
               
               
                 DAAGTVLVTGGTGALGAEVARHLVIERGVRNLVLVSRRGPAASGAAELVA 
               
               
                   
               
               
                 QLTAYGAEVSLQACDVADRETLAKVLASIPDEHPLTAVVHAAGVLDDGVS 
               
               
                   
               
               
                 ESLTVERLDQVLRPKVDGARNLLELIDPDVALVLFSSVSGVLGSGGQGNY 
               
               
                   
               
               
                 AAANSFLDALAQQRQSRGLPTRSLAWGPWAEHGMASTLREAEQDRLARSG 
               
               
                   
               
               
                 LLPISTEEGLSQFDAACGGAHTVVAPVRFSRLSDGNAIKFSVLQGLVGPH 
               
               
                   
               
               
                 RVNKAATADDAESLRKRLAALPEADRRRAVLDLVEELVLGVLGHETRAAI 
               
               
                   
               
               
                 GPDSSFHAIGFDSLTAVELRNLLTVRLGMKLPATLVYDHPTLSSLADHLH 
               
               
                   
               
               
                 EQLVIDGTPMTDTAADLLAELDALAARLAAVGLEPEARARIGRRLKDMQT 
               
               
                   
               
               
                 ACEPRSESSRDLKSASRTEVLDFLTNELGISR 
               
            
           
         
       
     
     The amino acid sequence of the S3c variant with ery TE is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 2) 
               
               
                 MAHEDKLRHLLKRVSAELDDTQRRVREMEESEREPIAIVGMSCRLPGGVN 
               
               
                   
               
               
                 SPGEFWSLLEAGTDAVSEFPRDRGWDVENLYDPDPDAPGRSYVREGGFLD 
               
               
                   
               
               
                 GAGQFDAAFFGISPREALAMDPQQRLLLECSWEAIERSRIDPKTLHGSRT 
               
               
                   
               
               
                 GVFAGSNWQDYNTLLLNAEERSQSYLATGASGSVLSGRVSYTLGMEGPAI 
               
               
                   
               
               
                 TVNTACSSSLVAVHLAARSLRAGECDLALAGAVTVMSTPQLPVAFSRQRG 
               
               
                   
               
               
                 LAPDGRSKAFAVSADGMGFGEGVGVLVLERLSVARRNGHRVLAVVRGSAV 
               
               
                   
               
               
                 NQDGASNGLTAPNGPSQQRVIRAALASAGLGPADVDVVEAHGTGTRLGDP 
               
               
                   
               
               
                 IEAQALLATYGRGRDAERPLWLGSVKSNIGHAQAAAGVAGVIKMVLAMEK 
               
               
                   
               
               
                 GRLPRTLHVDEPSGEVDWDSGAVRLLTEARDWPSEEGRLRRAGVSSFGIS 
               
               
                   
               
               
                 GTNAHVIIEEAPEEGEEPESDAGGVVPWVLSARTEGALQAQAVQLSEFVG 
               
               
                   
               
               
                 ESSPVDVGWSLVSTRAAFEHRAVVVGRGRDELVRGLSEVAQGRGVRGVAS 
               
               
                   
               
               
                 SASGGLAFVFAGQGSQRLGMGRGLYERFPVFAEAFDEVCGRVGPGVREVV 
               
               
                   
               
               
                 FGSDAGELDRTVWAQAGLFALEVALFRLLESWGVRPGCLIGHSVGELSAA 
               
               
                   
               
               
                 CVAGLWSLEDACRVVAARARLMQALPAGGVMVAVRAEAGELAGFLGEDVV 
               
               
                   
               
               
                 IASVNAPGQVVIAGPEGGVERVVAACGARSRRLAVSHAFHSPLVEPMLGE 
               
               
                   
               
               
                 FRRVVESVAFGVPSLRVVSNVTGAWVDPEEWGTPEYWVRQVREPVRFADG 
               
               
                   
               
               
                 VATLLDAGVRTFVELGPAGALTSMVSHCADATATSVTAVPTLRPDHDESR 
               
               
                   
               
               
                 TVLSAAASLYVQGHPVDWAPLFPRARTVDLPTYPFQHQHYWMMNTGSAAE 
               
               
                   
               
               
                 PAELGLGDARHPLLGSVVTVAGDDKVVFAGRLALRTHPWLADHTVLDAVL 
               
               
                   
               
               
                 LPATAFLELAVRAGEEVSCPVVHDLTLHRPLVVPERGAVQVQMAVGAPEA 
               
               
                   
               
               
                 DGRREVRVYSRPDDDAEHEWTLHAAGLLASAATAEPAVAAGAWPPPEAQA 
               
               
                   
               
               
                 VDLDGFYAGLAEHGYHYGPLFQGVRAAWRLGDDVLAEIVLPEAAGADAAR 
               
               
                   
               
               
                 YGMHPALLDAVLHAARLGAFRERSEEKYLPFAWEGVTLRTRGATAVRARI 
               
               
                   
               
               
                 SRAGTDAIRLDVTDTADRPVLTAESLVLRSAAARRTGARRQAHQARLYRL 
               
               
                   
               
               
                 SWPTVQLPTSAQPPSCVLLGTSEVSADIQVYPDLRSLTAALDAGAEPPGV 
               
               
                   
               
               
                 VIAPTPPGGGRTADVRETTRHALDLVQGWLSDQRLNESRLLLVTQGAVAV 
               
               
                   
               
               
                 EPGEPVTDLAQAALWGLLRSTQTEHPDRFVLVDVPEPAQLLPALPGVLAC 
               
               
                   
               
               
                 GEPQLALRRGGAHAPRLAGLGSDDVLPVPDGTGWRLEATRPGSLDGLALV 
               
               
                   
               
               
                 DEPTATAPLGDGEVRIAMRAAGVNFRDALIALGMYPGVASLGSEGAGVVV 
               
               
                   
               
               
                 ETGPGVTGLAPGDRVMGMIPKAFGPLAVADHRMVTRIPAGWSFARAASVP 
               
               
                   
               
               
                 IVFLTAYYALVDLAGLRPGESLLVHSAAGGVGMAAIQLARHLGAEVYATA 
               
               
                   
               
               
                 SEDKWQAVELSREHLASSRTCDFEQQFLGATGGRGVDVVLNSLAGEFADA 
               
               
                   
               
               
                 SLRMLPRGGRFLELGKTDVRDPVEVADAHPGVSYQAFDTVEAGPQRIGEM 
               
               
                   
               
               
                 LHELVELFEGRVLEPLPVTAWDVRQAPEALRHLSQARHVGKLVLTMPPVW 
               
               
                   
               
               
                 DAAGTVLVTGGTGALGAEVARHLVIERGVRNLVLVSRRGPAASGAAELVA 
               
               
                   
               
               
                 QLTAYGAEVSLQACDVADRETLAKVLASIPDEHPLTAVVHAAGVLDDGVS 
               
               
                   
               
               
                 ESLTVERLDQVLRPKVDGARNLLELIDPDVALVLFSSVSGVLGSGGQGNY 
               
               
                   
               
               
                 AAANSFLDALAQQRQSRGLPTRSLAWGPWAEHGMASTLREAEQDRLARSG 
               
               
                   
               
               
                 LLPISTEEGLSQFDAACGGAHTVVAPVRFSRLSDGNAIKFSVLQGLVGPH 
               
               
                   
               
               
                 RVNKAATADDAESLRKRLAALPEADRRRAVLDLVEELVLGVLGHETRAAI 
               
               
                   
               
               
                 GPDSSFHAIGFDSLTAVELRNLLTVRLGMKLPATLVYDHPTLSSLADHLH 
               
               
                   
               
               
                 EQLESGTPAREASSALRDGYRQAGVSGRVRSYLDLLAGLSDFREHFDGSD 
               
               
                   
               
               
                 GFSLDLVDMADGPGEVTVICCAGTAAISGPHEFTRLAGALRGIAPVRAVP 
               
               
                   
               
               
                 QPGYEEGEPLPSSMAAVAAVQADAVIRTQGDKPFVVAGHSAGALMAYALA 
               
               
                   
               
               
                 TELLDRGHPPRGVVLIDVYPPGHQDAMNAWLEELTATLFDRETVRMDDTR 
               
               
                   
               
               
                 LTALGAYDRLTGQWRPRETGLPTLLVSAGEPMGPWPDDSWKPTWPFEHDT 
               
               
                   
               
               
                 VAVPGDHFTMVQEHADAIARHIDAWLGGGNS* 
               
            
           
         
       
     
     The amino acid sequence of  Streptomyces parvulus  BorA1 is: 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 5) 
                   
               
               
                         10          20          30          40          50 
                   
               
               
                 MTGSAVSAPF  LQPPEPVSGH  SERKSDPVLL  VGAGRRARMA  DAVRAAGAQA 
               
               
                   
               
               
                         60          70          80          90         100 
               
               
                 GIDPAVLRRT  RATLITAGSA  GAAGRLAAAL  RLTGATISLD  TRETPTLLAL 
               
               
                   
               
               
                        110         120         130         140         150 
               
               
                 HLAAQALRAG  DTSYAVVGAE  LPDGNCALIL  ARQSAATAEG  AVPQAIVRTT 
               
               
                   
               
               
                        160         170         180         190         200 
               
               
                 TADRTTTADH  APAPDDHGSP  AREAPHATRT  LSPGITQAPA  EGFPGLLATL 
               
               
                   
               
               
                        210         220         230         240         250 
               
               
                 HDDTPLRPTA  VTEHGSDATT  VLVLLDQPQD  AAPAAPLPWV  VSAPHTRALR 
               
               
                   
               
               
                        260         270         280         290         300 
               
               
                 ATAATLAVHL  DTTPAAPADV  AHTLLTARPD  RHRAAVVGAD  RATLTDGLRA 
               
               
                   
               
               
                        310         320         330         340         350 
               
               
                 LATGGDAPHL  VHGTATGSPR  PVFVFPGQGS  QWPGMAAELL  ETSEPFHDSV 
               
               
                   
               
               
                        360         370         380         390         400 
               
               
                 HACADALAEF  VDWSVLDVLR  QAPDAPPLRR  VDVLQPTLWA  TMVSLAEVWR 
               
               
                   
               
               
                        410         420         430         440         450 
               
               
                 SYGVEPAAVV  GHCCGEIAAA  QVAGALDMRD  AARLLAHRSR  AWLRLVGKGT 
               
               
                   
               
               
                        460         470         480         490         500 
               
               
                 VISVATSGQD  ITRRMAAWPD  SVELAALNGP  RSVALAGPPD  VLDGIVNDLT 
               
               
                   
               
               
                        510         520         530         540         550 
               
               
                 DQGIHAKRIP  GVDTVGHCSQ  VEVLRDHLLD  VLRPVSPRPA  AVPFYSTVDG 
               
               
                   
               
               
                        560         570         580         590         600 
               
               
                 TERDTTTLDT  DYWYLNTRSQ  VRFHQAVRNL  LAAGHRSFVE  VSPHPLLGAS 
               
               
                   
               
               
                        610         620         630         640         650 
               
               
                 IEDTAAEFGL  DDVAAVGTLR  RGQGGTRRVL  TSVAEAYVHG  IDIDFTPAFT 
               
               
                   
               
               
                        660         670         680         690         700 
               
               
                 GTTPNRIDLP  TVEDHGIEGH  GDDGGETWTD  RVRTLPDEQR  EEALLDLVCR 
               
               
                   
               
               
                        710         720         730         740         750 
               
               
                 TVAAVLEADP  AGTADAVAPD  TAFKEMGLGS  LSAVRLRNGL  REATGAHLPA 
               
               
                   
               
               
                        760         770         780         790         800 
               
               
                 TIAYDHPTPA  ALARHLAMTL  FDATGAAPAV  PAPSRDDEPI  DAETAVLTAL 
               
               
                   
               
               
                        810         820         830         840         850 
               
               
                 ERADEALERL  RAPHARTPRQ  ETGRRIDELL  RSLTDKARRM  RQADAVDDVD 
               
               
                   
               
               
                        860         870 
               
               
                 DPATDRFAAA  TDDEMFELLE  KRFGIS 
               
            
           
         
       
     
     The amino acid sequence of  Streptomyces parvulus  BorA2 is: 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 6) 
                   
               
               
                         10          20          30          40          50 
                   
               
               
                 MAHEDKLRHL  LKRVSAELDD  TQRRVREMEE  SEREPIAIVG  MSCRLPGGVN 
               
               
                   
               
               
                         60          70          80          90         100 
               
               
                 SPGEFWSLLE  AGTDAVSEFP  RDRGWDVENL  YDPDPDAPGR  SYVREGGFLD 
               
               
                   
               
               
                        110         120         130         140         150 
               
               
                 GAGQFDAAFF  GISPREALAM  DPQQRLLLEC  SWEAIERSRI  DPKTLHGSRT 
               
               
                   
               
               
                        160         170         180         190         200 
               
               
                 GVFAGSNWQD  YNTLLLNAEE  RSQSYLATGA  SGSVLSGRVS  YTLGMEGPAI 
               
               
                   
               
               
                        210         220         230         240         250 
               
               
                 TVNTACSSSL  VAVHLAARSL  RAGECDLALA  GAVTVMSTPQ  LPVAFSRQRG 
               
               
                   
               
               
                        260         270         280         290         300 
               
               
                 LAPDGRSKAF  AVSADGMGFG  EGVGVLVLER  LSVARRNGHR  VLAVVRGSAV 
               
               
                   
               
               
                        310         320         330         340         350 
               
               
                 NQDGASNGLT  APNGPSQQRV  IRAALASAGL  GPADVDVVEA  HGTGTRLGDP 
               
               
                   
               
               
                        360         370         380         390         400 
               
               
                 IEAQALLATY  GRGRDAERPL  WLGSVKSNIG  HAQAAAGVAG  VIKMVLAMEK 
               
               
                   
               
               
                        410         420         430         440         450 
               
               
                 GRLPRTLHVD  EPSGEVDWDS  GAVRLLTEAR  DWPSEEGRLR  RAGVSSFGIS 
               
               
                   
               
               
                        460         470         480         490         500 
               
               
                 GTNAHVIIEE  APEEGEEPES  DAGGVVPWVL  SARTEGALQA  QAVQLSEFVG 
               
               
                   
               
               
                        510         520         530         540         550 
               
               
                 ESSPVDVGWS  LVSTRAAFEH  RAVVVGRGRD  ELVRGLSEVA  QGRGVRGVAS 
               
               
                   
               
               
                        560         570         580         590         600 
               
               
                 SASGGLAFVF  AGQGSQRLGM  GRGLYERFPV  FAEAFDEVCG  RVGPGVREVV 
               
               
                   
               
               
                        610         620         630         640         650 
               
               
                 FGSDAGELDR  TVWAQAGLFA  LEVALFRLLE  SWGVRPGCLI  GHSVGELSAA 
               
               
                   
               
               
                        660         670         680         690         700 
               
               
                 CVAGLWSLED  ACRVVAARAR  LMQALPAGGV  MVAVRAEAGE  LAGFLGEDVV 
               
               
                   
               
               
                        710         720         730         740         750 
               
               
                 IASVNAPGQV  VIAGPEGGVE  RVVAACGARS  RRLAVSHAFH  SPLVEPMLGE 
               
               
                   
               
               
                        760         770         780         790         800 
               
               
                 FRRVVESVAF  GVPSLRVVSN  VTGAWVDPEE  WGTPEYWVRQ  VREPVRFADG 
               
               
                   
               
               
                        810         820         830         840         850 
               
               
                 VATLLDAGVR  TFVELGPAGA  LTSMVSHCAD  ATATSVTAVP  TLRPDHDESR 
               
               
                   
               
               
                        860         870         880         890         900 
               
               
                 TVLSAAASLY  VQGHPVDWAP  LFPRARTVDL  PTYPFQHQHY  WLDVPPLFTA 
               
               
                   
               
               
                        910         920         930         940         950 
               
               
                 SSAAQDGGWR  YRIHWRRLGT  RDSGDRLSGR  WLLLVPESDG  TEPWVEGAEK 
               
               
                   
               
               
                        960         970         980         990        1000 
               
               
                 MLAERGCEVV  HVPIAATADR  DAMVGAVRES  VEDGRVDGVL  SLLALDGRPH 
               
               
                   
               
               
                       1010        1020        1030        1040        1050 
               
               
                 PDAAAVPTGL  VATAQVVQVS  DELGIGPLWV  ATRQAVSVDG  ADEADGAGRT 
               
               
                   
               
               
                       1060        1070        1080        1090        1100 
               
               
                 RKADDPADVA  QAAVWGLGRV  AALEKPRLWG  GLVDLPARAD  ERMRDLVAQA 
               
               
                   
               
               
                       1110        1120        1130        1140        1150 
               
               
                 LTAPDAEDQL  AVRADGIAVR  RLVRSAASAP  ADDWQPSGTV  LVTGGTGGVG 
               
               
                   
               
               
                       1160        1170        1180        1190        1200 
               
               
                 ANVARWLVTQ  DIQHLLLVSR  RGPDAPGAAE  LLAELSASGT  SVTIEPCDVT 
               
               
                   
               
               
                       1210        1220        1230        1240        1250 
               
               
                 DADAVRRLIG  AVPAERPLST  VVHAAGVLDD  CLIDALTPQR  LAAALEVKAK 
               
               
                   
               
               
                       1260        1270        1280        1290        1300 
               
               
                 GALNLHEAAG  EAHLVLFSSL  AGTTGTKGQG  NYAAANAYLD  ALAERRRADG 
               
               
                   
               
               
                       1310        1320        1330        1340        1350 
               
               
                 LPATSVAWGA  WQGAGMVADA  AVAHRTRRYG  LPLMSPDRAV  ATLRQVMAEP 
               
               
                   
               
               
                       1360        1370        1380        1390        1400 
               
               
                 VATQVVADVD  WQRFVADFTA  VRPSRLLADL  PEVRSLGEQR  KDGPGGQGEE 
               
               
                   
               
               
                       1410        1420        1430        1440        1450 
               
               
                 DGLASKLAAL  PEADRRRAVL  DLVEELVLGV  LGHETRAAIG  PDSSFHAIGF 
               
               
                   
               
               
                       1460        1470        1480        1490        1500 
               
               
                 DSLTAVELRN  LLTVRLGMKL  PATLVYDHPT  LSSLADHLHE  QLVIDGTPMT 
               
               
                   
               
               
                       1510        1520        1530        1540        1550 
               
               
                 DTAADLLAEL  DALAARLAAV  GLEPEARARI  GRRLKDMQTA  CEPRSESSRD 
               
               
                   
               
               
                       1560        1570 
               
               
                 LKSASRTEVL  DFLTNELGIS  R 
               
            
           
         
       
     
     The amino acid sequence of  Streptomyces thioluteus  AurB is: 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 7) 
                   
               
               
                         10          20          30          40          50 
                   
               
               
                 MTNDAKTLEY  LKRLTAELLE  TRERLRTAEA  ADQEPVAVVS  MGCRYPGGVS 
               
               
                   
               
               
                         60          70          80          90         100 
               
               
                 SPEDLWRLVT  DGTDAIAPFP  ADRGWNVDDL  FDPDPDRPGR  TYTLEGGFVD 
               
               
                   
               
               
                        110         120         130         140         150 
               
               
                 GAAEFDADLF  GISPREATAM  DPQQRLLLET  AWETFERAGT  DPGSLRGRPV 
               
               
                   
               
               
                        160         170         180         190         200 
               
               
                 GVFVGSLFVA  GGSGVGVAEG  AEGYHMTGNA  ASVLSGRLAY  AFGLEGPAVT 
               
               
                   
               
               
                        210         220         230         240         250 
               
               
                 VDTACSASLV  AVHQAVQALR  QGECALALAG  GSTVMTTPGV  FTEFSRQRGL 
               
               
                   
               
               
                        260         270         280         290         300 
               
               
                 APDGRCKAFA  TAADGTGFGE  GVGLVLLEKL  SDARKNGHPV  LAVIRGSAVN 
               
               
                   
               
               
                        310         320         330         340         350 
               
               
                 QDGASNGLTA  PNGPSQQRVI  RQALAAARVS  ADEVDVVEAH  GTGTGLGDPV 
               
               
                   
               
               
                        360         370         380         390         400 
               
               
                 EAQALLATYG  QGRPDDRPLW  LGSIKSNLGH  TQGAAGVAGL  IKMVMAVRHG 
               
               
                   
               
               
                        410         420         430         440         450 
               
               
                 VLPMTLHVDE  PSAHVDWDSG  AVRLLTGNHD  WPETGRPRRA  GVSSFGISGT 
               
               
                   
               
               
                        460         470         480         490         500 
               
               
                 NAHLILEQAP  DAEESDAEPA  SGAPARIPWV  LAARGEEALR  AQAERLLTEV 
               
               
                   
               
               
                        510         520         530         540         550 
               
               
                 RDRPELRPVD  VGHALATSRA  ALDQRAVVWA  DGRDGLLAAL  TALAEERPAP 
               
               
                   
               
               
                        560         570         580         590         600 
               
               
                 GVVHGTVADG  RLAFLFSGQG  SQRPGMGHEL  TESFPVFAEK  LDEVCGHLDR 
               
               
                   
               
               
                        610         620         630         640         650 
               
               
                 HLDRPLRELL  FAAEGTPEAA  LLEQTGYTQA  ALFAHEVALH  HLLTHWGITP 
               
               
                   
               
               
                        660         670         680         690         700 
               
               
                 DLLLGHSIGE  LTAAHVAGVL  SLEDACALVA  ARGRLMQQLP  GAGAMLSVQA 
               
               
                   
               
               
                        710         720         730         740         750 
               
               
                 TEAEVLPWVT  EHAHEMSIAA  VNGPRSVVVS  GAESAVLEFA  EHWKNEGRKT 
               
               
                   
               
               
                        760         770         780         790         800 
               
               
                 KRLRVSHAFH  SPQMDGMLQE  FARVAEKLAF  HPPRIPVVSN  VTGEVATAEQ 
               
               
                   
               
               
                        810         820         830         840         850 
               
               
                 LCSPAYWVRH  AREAVRFHDG  IRRLVAEGAH  VFLEVGPSGV  LTAMAQDCLA 
               
               
                   
               
               
                        860         870         880         890         900 
               
               
                 DEPGTVTAAV  SRGGRPEADA  ALAAVAEAYV  HGVRVDWDRF  FAGTGARRID 
               
               
                   
               
               
                        910         920         930         940         950 
               
               
                 LPTYAFRRRS  FPWIQAAPDA  DVTTAGLAGL  GHPLLGASLE  LADAQGAALS 
               
               
                   
               
               
                        960         970         980         990        1000 
               
               
                 GRLSARTESW  LADHVVLGST  LVPGTAVVEM  AVRAGAETGC  GRLAELTQEA 
               
               
                   
               
               
                       1010        1020        1030        1040        1050 
               
               
                 PLAVPERGAV  HLQVRVGPAG  EQGHRPVGVY  SRPEDAEPDE  PWACHARGVL 
               
               
                   
               
               
                       1060        1070        1080        1090        1100 
               
               
                 APEAAPVPAG  TGGAWPPSGA  EPVPLDGFYE  RLAAEGFAYG  PAFQGLTRAW 
               
               
                   
               
               
                       1110        1120        1130        1140        1150 
               
               
                 RLGDEVLAEI  TLPEGACSGA  DRYGVHPALL  DAALHTALLK  EEASDTSQVR 
               
               
                   
               
               
                       1160        1170        1180        1190        1200 
               
               
                 IPFAWHEVSF  HGGSAPVLRA  RLTPSGTDTV  SLALWDEHGT  PVASVGSLVS 
               
               
                   
               
               
                       1210        1220        1230        1240        1250 
               
               
                 RPVSARQLRA  TRTHDTLFRL  DWVETTITPA  AARCAVLGDD  ELAGALSVPA 
               
               
                   
               
               
                       1260        1270        1280        1290        1300 
               
               
                 FADLAALESA  DPVPELVLYP  CLGDDAEDDR  ADAARSLTAR  VLGVLQAWVA 
               
               
                   
               
               
                       1310        1320        1330        1340        1350 
               
               
                 DERWATTRLA  LVTRGAMSVT  DREQVTDLPA  AAVWGLVRSA  QAEHPGRFVL 
               
               
                   
               
               
                       1360        1370        1380        1390        1400 
               
               
                 ADLDGDTASA  AALPGILAAS  GDEPQLALRE  GAVLVPRLAR  GVPSGTLVPP 
               
               
                   
               
               
                       1410        1420        1430        1440        1450 
               
               
                 PGTRDWHLEL  TGGGTVDDLA  LTPFPEAAAP  LAPGQVRVAV  RAAGLNFRDV 
               
               
                   
               
               
                       1460        1470        1480        1490        1500 
               
               
                 VMALGMVDDR  RALGGEIAGI  VTEAGPGVTG  FAPGDRVFGL  ADGCIGPVAV 
               
               
                   
               
               
                       1510        1520        1530        1540        1550 
               
               
                 VDHRLIARIP  EGWSFPQAAS  VPVTFLTAYY  GLVDLAGVRP  GDRVLVHAAA 
               
               
                   
               
               
                       1560        1570        1580        1590        1600 
               
               
                 GGVGMAAVQL  ARHLGAEVFA  TAGPAKWDTV  RALGIDDDHL  ASSRTDEFET 
               
               
                   
               
               
                       1610        1620        1630        1640        1650 
               
               
                 RFAAEDGGRG  IDVVLNSLAG  EMADASLRLV  RPGGRFIEMG  KTDIRDADEV 
               
               
                   
               
               
                       1660        1670        1680        1690        1700 
               
               
                 AAAYEGVVYR  AFDLMDGGAE  CIARIFAELL  ALFEGGKIQL  VPVTTWDVRQ 
               
               
                   
               
               
                       1710        1720        1730        1740        1750 
               
               
                 APEAFRYFAQ  ARHVGKIVLT  VPPAWDPEGT  VLVTGASGGV  AAHLVRHLVR 
               
               
                   
               
               
                       1760        1770        1780        1790        1800 
               
               
                 THDVRHLLLA  SRRGPDAEGM  DELIAELRES  GAHSVRAVAC  DCVDRTAVAD 
               
               
                   
               
               
                       1810        1820        1830        1840        1850 
               
               
                 LLASIPDEHP  LTAVVHTVGV  VDDGVLETMT  PERIDAVFRP  KADGAWHLHE 
               
               
                   
               
               
                       1860        1870        1880        1890        1900 
               
               
                 LTRDRDLAAF  AVCSSVAGTL  GSAAQANYAA  ANAFLDALAA  HRRDHGLPAT 
               
               
                   
               
               
                       1910        1920        1930        1940        1950 
               
               
                 SLAWGMWAGT  GGMAANLSRA  DLDRMQRSGI  SGLSTEEGLA  LFDAALAAGR 
               
               
                   
               
               
                       1960        1970        1980        1990        2000 
               
               
                 PVWLPARLDA  KALRTAAGGG  SLPAPLRGLV  HVPAADAGPL  PAADALRGRL 
               
               
                   
               
               
                       20 10        20 20        2030       2040       2050 
               
               
                 ASLAPEERHE  AVLDVVRAQV  AVVLGHGAPE  GIDPQRAFKD  LGFDSLTAVE 
               
               
                   
               
               
                       20 60        20 70        2080       2090       2100 
               
               
                 LRNRLNAAAG  LTLPATLVFD  HPTPAALTDH  IESVLLAGLG  SPADPLLARL 
               
               
                   
               
               
                       2110        2120        2130        2140        2150 
               
               
                 DDWAAGLAAT  ALDDDERERV  AARLRALAGQ  WGAPDDGATS  IADELDGATD 
               
               
                   
               
               
                       2160 
               
               
                 DEVLDFISNE  LGIS 
               
            
           
         
       
     
     The amino acid sequence of  Streptomyces antibioticus  IdmO is: 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 8) 
                   
               
               
                         10          20          30          40          50 
                   
               
               
                 MHMVGVEEKL  RDYLRRVTGE  LSETRQRLKE  AEAESREPIA  IVSMACRFPG 
               
               
                   
               
               
                         60          70          80          90         100 
               
               
                 GIESPQDYWR  LLAEGRDAVA  GFPDDRGWDL  DNLFDPDPDA  PGKSYAREGA 
               
               
                   
               
               
                        110         120         130         140         150 
               
               
                 FVHGASEFDA  ELFGISPREA  LSMDPQQRLL  LEAAWEVFER  AGLDPGALKG 
               
               
                   
               
               
                        160         170         180         190         200 
               
               
                 RDIGVFAGAA  WSDYVSGSRK  VPDSAEGYAI  TGGSSSVLSG  RVAYTFGLEG 
               
               
                   
               
               
                        210         220         230         240         250 
               
               
                 PAVTVDTACS  SSLVAMHLAS  QALRSGECSM  ALAGGVSVLV  SPYPFVGFSR 
               
               
                   
               
               
                        260         270         280         290         300 
               
               
                 QRGLAPDGRC  KPFADRADGT  GWGEGVGMLL  LERLSDARRN  GHEVLAVLRG 
               
               
                   
               
               
                        310         320         330         340         350 
               
               
                 SAVNQDGASS  GLTAPNGPSQ  QRVIRAALAN  AGLTASDVDA  VEAHGTGTSL 
               
               
                   
               
               
                        360         370         380         390         400 
               
               
                 GDPIEAQALL  ATYGQGRPEG  RPLWLGSVKS  NIAHTQATAG  AAGVIKMVLA 
               
               
                   
               
               
                        410         420         430         440         450 
               
               
                 MRHGLLPKSL  HVDAPSTNVD  WSAGAVELLT  VAREWPEVDR  PWRAGVSSFG 
               
               
                   
               
               
                        460         470         480         490         500 
               
               
                 VSGTNAHVIV  EEAPESSADA  VAESGVRVPV  PVVPWVVSAR  SAEGLAAQAE 
               
               
                   
               
               
                        510         520         530         540         550 
               
               
                 RLARFVGERS  DQDPVDIGFS  LVRSRSLLEH  RAVVLGKGRD  DLVAGLASLA 
               
               
                   
               
               
                        560         570         580         590         600 
               
               
                 SDGSATGVVS  GVARGRARVA  FGFSGQGAQR  VGMGAELASV  YPVFAEALAE 
               
               
                   
               
               
                        610         620         630         640         650 
               
               
                 VTGALGLDPE  VFGDVDRLGR  TEVTQAALFA  FEVAVVRLLE  SFGVRPDVLI 
               
               
                   
               
               
                        660         670         680         690         700 
               
               
                 GHSIGEIAAA  YVAGVFSLGD  AAALVGARGR  LMQALPAGGV  MVAVQAGEAE 
               
               
                   
               
               
                        710         720         730         740         750 
               
               
                 VVAALEGFAD  RVSLAAVNGP  SSVVVSGEAE  AVEQVVARLG  KVKSKRLRVS 
               
               
                   
               
               
                        760         770         780         790         800 
               
               
                 HAFHSPLMEP  MLADFRQVAE  QITYNEPQLP  VVSNVSGRLA  EPGELTTPDY 
               
               
                   
               
               
                        810         820         830         840         850 
               
               
                 WVRHVREAVR  FGDGVRALAA  DGVGVLVEVG  PDSVLTALAR  ESLDGEDGLR 
               
               
                   
               
               
                        860         870         880         890         900 
               
               
                 AVPLLRKDRP  EPETLLTGVA  QAFTHGVQVD  WPALLPGGRR  VELPTYAFQR 
               
               
                   
               
               
                        910         920         930         940         950 
               
               
                 RRYWLEDADP  TGGDPAALGL  TAADHPLLGA  AVPLAEDQGI  VITSRLSLRT 
               
               
                   
               
               
                        960         970         980         990        1000 
               
               
                 HPWLADHEIG  GTVLLPGAGL  VEIALRAGDE  VGCGRVEELT  LEIPLVVPQE 
               
               
                   
               
               
                       1010        1020        1030        1040        1050 
               
               
                 GGVTVQIRVG  APDESGWRPM  TVHSRTDPEE  EWTRHVSGVL  SPDVPTERYD 
               
               
                   
               
               
                       1060        1070        1080        1090        1100 
               
               
                 LGAWPPAGAT  PVELDGFYEA  YARLGYAYGP  SFQGLRAAWR  RGDEVFAEVS 
               
               
                   
               
               
                       1110        1120        1130        1140        1150 
               
               
                 LPVEEQETAG  RFTLHPALLD  AALQSAGAGA  FFDSGGSMRL  PFAWSGVSVF 
               
               
                   
               
               
                       1160        1170        1180        1190        1200 
               
               
                 AAGASTVRVR  LSPAGPDAVT  VALADPTGAP  VALVERLLIP  EMSPEQLERV 
               
               
                   
               
               
                       1210        1220        1230        1240        1250 
               
               
                 RGEEKEAPYV  LDWVPVEVPA  DDLVRPERWT  LLGGADAGVG  LDVAGAFASL 
               
               
                   
               
               
                       1260        1270        1280        1290        1300 
               
               
                 EPSDGAPEFV  VLPCVPPTSP  TRAADVRQST  LQALTVLQNW  VTDERHADSR 
               
               
                   
               
               
                       1310        1320        1330        1340        1350 
               
               
                 LVLVTRRAVG  VGAHDDVPDL  THAALWGLVR  SAQTENPGRF  LLVDLDEGAE 
               
               
                   
               
               
                       1360        1370        1380        1390        1400 
               
               
                 LAEVLPGALG  SGESQVAVRA  GRVLAARLAR  SGSGGAELVP  PAGAPWRLDT 
               
               
                   
               
               
                       1410        1420        1430        1440        1450 
               
               
                 TSPGTLENLA  LVPSAEEPLG  PLDVRVSVRA  AGLNFRDVLI  ALGMYPGDAR 
               
               
                   
               
               
                       1460        1470        1480        1490        1500 
               
               
                 MGGEGAGVVT  DVGSEVTTLA  PGDRVMGMLS  SAFGPTAVSD  HRALVRVPDD 
               
               
                   
               
               
                       1510        1520        1530        1540        1550 
               
               
                 WSFEQAASVP  TVFATAYYGL  VDLAELRAGQ  SVLVHAAAGG  VGMAAVQLAR 
               
               
                   
               
               
                       1560        1570        1580        1590        1600 
               
               
                 HLGAEVFGTA  STGKWDSLRA  GGLDAEHIAS  SRTVEFEETF  LAATAGRGVD 
               
               
                   
               
               
                       1610        1620        1630        1640        1650 
               
               
                 VVLDSLAGEF  VDASLRLLPR  GGRFVEMGKA  DIRDAERVAA  DHPGVTYRSF 
               
               
                   
               
               
                       1660        1670        1680        1690        1700 
               
               
                 DLLEAGLDRF  QEILTEVVRL  FERGVLRHLP  VTAWDVRRAA  EAFRFVSQAR 
               
               
                   
               
               
                       1710        1720        1730        1740        1750 
               
               
                 HVGKNVLVMP  RVWDRDGTVL  ITGGTGALGA  LVARHLVAEH  GMRNVLLAGR 
               
               
                   
               
               
                       1760        1770        1780        1790        1800 
               
               
                 RGVDAPGARE  LLAELETAGA  QVSVVACDVA  DRDAVAELIA  KVPVEHPLTA 
               
               
                   
               
               
                       1810        1820        1830        1840        1850 
               
               
                 VVHTAGVVAD  ATLTALDAER  VDTVLRAKVD  AVLHLHEATR  GLDLAGFVLF 
               
               
                   
               
               
                       1860        1870        1880        1890        1900 
               
               
                 SSASGIFGSP  GQGNYAAANS  FIDAFAHHRR  AQGLPALSLA  WGLWARTSGM 
               
               
                   
               
               
                       1910        1920        1930        1940        1950 
               
               
                 AGQLGHDDVA  RISRTGLAPI  TDDQGMALLD  AALGAGRPLL  VPVRLDRAAL 
               
               
                   
               
               
                       1960        1970        1980        1990        2000 
               
               
                 RSQATAGTLP  PILRGLVRAT  VRRAASTAAA  QGPSLAERLA  GLPVTEHERI 
               
               
                   
               
               
                       2010        2020        2030        2040        2050 
               
               
                 VVELVRADLA  AVLGHSSSAG  IDPGRAFQDM  GIDSLTAVEL  RNRLNGATGL 
               
               
                   
               
               
                       2060        2070        2080        2090        2100 
               
               
                 RLAASLVFDY  PTPNALATHI  LDELALDTAG  AGAAGEPDGP  APAPADEARF 
               
               
                   
               
               
                       2110        2120        2130        2140        2150 
               
               
                 RRVINSIPLD  RIRRAGLLDA  LLGLAGTSAD  TAASDDFDQE  EDGPAIASMD 
               
               
                   
               
               
                       2160        2170 
               
               
                 VDDLVRIALG  ESDTTADITE  GTDRS 
               
            
           
         
       
     
     The amino acid sequence of  Streptomyces nanchangensis  NanA2 is: 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 9) 
                   
               
               
                         10          20          30          40          50 
                   
               
               
                 MVSEEKLVEY  LRRVTTELHD  ARTRLRELEE  GEQEPVAVVG  MACRFPGGVR 
               
               
                   
               
               
                         60          70          80          90         100 
               
               
                 SPEDLRRLVL  SGGDAIGDFP  TDRGWDLDGL  FHPDPAHFGT  SYVSQGGFLY 
               
               
                   
               
               
                        110         120         130         140         150 
               
               
                 DVDRFDAGFF  GISPREALAM  DPQQRLLLEL  SWEALESAGV  VPGALRASRT 
               
               
                   
               
               
                        160         170         180         190         200 
               
               
                 GVYVGVSSED  YISGLPQIPE  GFEGYATTGS  LTSVISGRVA  YTFGFEGPAV 
               
               
                   
               
               
                        210         220         230         240         250 
               
               
                 TVDTACSSSM  VAIHLAGQAL  RQGECSLALA  GGVTVLSTPL  MFTEFCRQRA 
               
               
                   
               
               
                        260         270         280         290         300 
               
               
                 LTPDARCKPF  AAAADGTGFS  EGAGLLLLER  LSDARRNGHE  VLAVLRGSAI 
               
               
                   
               
               
                        310         320         330         340         350 
               
               
                 NQDGASNGLT  APNDVAQESV  IRDALARAGL  SGADVDMVEA  HGTGTRLGDP 
               
               
                   
               
               
                        360         370         380         390         400 
               
               
                 IEAEALIATY  GADRPADRPL  YLGSIKSNIG  HTHAAAGVAG  AINTVMALRD 
               
               
                   
               
               
                        410         420         430         440         450 
               
               
                 GKLARTLHID  EPTRHVDWSA  GTVRLLTDPY  DWPVADRPRR  AAVSSFGVSG 
               
               
                   
               
               
                        460         470         480         490         500 
               
               
                 TNAHVILEQA  PDAGAQQDAR  QRGGDTFHGV  VPWPVSGRTE  AALRDQAARL 
               
               
                   
               
               
                        510         520         530         540         550 
               
               
                 GAFLTADGAT  ANGAATGGVA  DVGWSLAMRR  TAFEHRAVVV  GRDRSDLLAA 
               
               
                   
               
               
                        560         570         580         590         600 
               
               
                 LEGLAADEPG  PAVVRGVAAD  VGAGPVMVFP  GQGSQWLGMG  VELLDSSPVF 
               
               
                   
               
               
                        610         620         630         640         650 
               
               
                 AARIAACERA  LAAHVDWSLT  DVLRGARGAA  DIGRVDVVQP  VLWAVMVSLA 
               
               
                   
               
               
                        660         670         680         690         700 
               
               
                 AVWEAHGVRP  SAVVGHSQGE  IAAACVAGAM  TLEDGARVVA  LRARALRALA 
               
               
                   
               
               
                        710         720         730         740         750 
               
               
                 GYGAMASLGC  GVEETERLTA  VHAPDVAVAA  VNGPSSTVVS  GPSEQVEKLV 
               
               
                   
               
               
                        760         770         780         790         800 
               
               
                 AAVRADGLRA  RAIDVDYASH  GPQVDRIADE  LADVLAGVSG  AATDTAFYST 
               
               
                   
               
               
                        810         820         830         840         850 
               
               
                 VTGARMDASG  LDAGYWFTNL  RQPVRFAEAV  QALLDADYRV  FIEVSAHPVL 
               
               
                   
               
               
                        860         870         880         890         900 
               
               
                 LLGLQECFEA  AGRPAVAIGT  LRRDEGGPER  LCRALAEAHV  AGVAVDWASW 
               
               
                   
               
               
                        910         920         930         940         950 
               
               
                 YADGPAPAAV  PLPAYAFQRE  RYWLPAGAGS  GPGDVAGAGL  TAVGHALLPV 
               
               
                   
               
               
                        960         970         980         990        1000 
               
               
                 SVRLADGSLV  LTGRLPEAAR  AGWLAEHLVA  DLPLLPGTVL  VEWVLRAADE 
               
               
                   
               
               
                       1010        1020        1030        1040        1050 
               
               
                 AGCGGVEELA  LQVPVALPVS  GGLVIQVVVD  AAEGDGRRPV  RVHSRPEEDS 
               
               
                   
               
               
                       1060        1070        1080        1090        1100 
               
               
                 GAPDAWVCHV  SGTLLPGVAG  PVPPSGPGGA  WPPPGARPAA  IDGFYERAEA 
               
               
                   
               
               
                       1110        1120        1130        1140        1150 
               
               
                 AGYGYGAFFR  GLTNVWHDGE  DTLAEVVLPK  EAAEQAGGFG  IHPALLDAAM 
               
               
                   
               
               
                       1160        1170        1180        1190        1200 
               
               
                 QPVLLAGQLR  QCAAAAGADT  ASGTVLLPFT  WSGVRLWAGG  ATRLRVRLSP 
               
               
                   
               
               
                       1210        1220        1230        1240        1250 
               
               
                 RPEGLRVLLA  DATGAPVLTA  DAVALRETGV  QQLRASSRVR  GSHGLFAVEW 
               
               
                   
               
               
                       1260        1270        1280        1290        1300 
               
               
                 VPPLSATAGG  TAPATLAVLG  DDAPDLADAD  RYPDLDALFR  AVADGAPAPD 
               
               
                   
               
               
                       1310        1320        1330        1340        1350 
               
               
                 VVIASVRTGN  DPAGSDTGLA  TARRTLTLAQ  EWLAGSGADG  ARLAVVTRSA 
               
               
                   
               
               
                       1360        1370        1380        1390        1400 
               
               
                 IRTGDDGQER  VVPSAAAVWG  LMRSAQTEHP  GRFVLIDEDT  DSTENILEAV 
               
               
                   
               
               
                       1410        1420        1430        1440        1450 
               
               
                 RTDEPQLALR  GGRALVPRMA  RVDAEPELTA  PSGERAWHVA  AGKTGPDDLT 
               
               
                   
               
               
                       1460        1470        1480        1490        1500 
               
               
                 AVPSPRASAP  LAPGQVRIAV  RAAGLNFRDA  LIALDMYPDA  SASIGSEGAG 
               
               
                   
               
               
                       1510        1520        1530        1540        1550 
               
               
                 VVLEVSEGVA  GVAVGDRVMG  LFNDAFGPVA  VADARMVAPV  PDGWSFREAA 
               
               
                   
               
               
                       1560        1570        1580        1590        1600 
               
               
                 AAPVAFLTAW  YGLVDLGGLS  SGETVVIHGA  AGGVGMAAVQ  VARHLGAEVF 
               
               
                   
               
               
                       1610        1620        1630        1640        1650 
               
               
                 ATASPAKHPV  LEGMGVDAAH  RASSRDLGFE  AAFSSATGGR  GVDVVLNSLA 
               
               
                   
               
               
                       1660        1670        1680        1690        1700 
               
               
                 GEFTDASLRL  LAPGGRLIEM  GKTDVRDPDQ  VAREHSVAYR  AFDLIADAGP 
               
               
                   
               
               
                       1710        1720        1730        1740        1750 
               
               
                 ERIGQLLAAL  GERFADGAFT  PLPVTGWRLG  QARQALRQLS  QARHTGKLVL 
               
               
                   
               
               
                       1760        1770        1780        1790        1800 
               
               
                 DVDPAPDPDG  TVLITGGTGT  LGGLIAEHLV  RSRGVRHLLL  LSRRGPDAPG 
               
               
                   
               
               
                       1810        1820        1830        1840        1850 
               
               
                 AEELTARLTE  LGARVRVAAV  DVGDATALGE  AVAGVDPAHP  LTGVVHAAGV 
               
               
                   
               
               
                       1860        1870        1880        1890        1900 
               
               
                 VADAMLPSQD  DERLVAAWSA  KAAAAARLHD  ATAGLPLGMF  VLFSSFASTL 
               
               
                   
               
               
                       1910        1920        1930        1940        1950 
               
               
                 GTAGQANYAA  ANAYCDALVE  RRHAEGLPGV  SVSWGLWSAA  SGLTGGLTEA 
               
               
                   
               
               
                       1960        1970        1980        1990        2000 
               
               
                 DVARIARQGI  VPNSTEQGYD  LFDAALGHGR  PALLALNLDT  RALAAQPVAA 
               
               
                   
               
               
                       2010        2020        2030        2040        2050 
               
               
                 LPAPLRALAA  DAQAAGARSG  GAAARPTAAA  AEEPADWAAR  LRALAPAEQR 
               
               
                   
               
               
                       2060        2070        2080        2090        2100 
               
               
                 RLLTDLVRRH  AATVLGHADP  EAVPADAAFK  ELGFDSLTAV  ELRNRVTAAT 
               
               
                   
               
               
                       2110        2120        2130        2140        2150 
               
               
                 GLRLPATVIF  DYPEPGALAE  RLRTELAPEE  GASATAPDLY  APVLSRLTGL 
               
               
                   
               
               
                       2160        2170        2180        2190        2200 
               
               
                 EETLAALASS  GVNGGVNGGV  ADPGAVTARL  ESLLADWKAA  HAPSRNGGTA 
               
               
                   
               
               
                       2210        2220 
               
               
                 AERLEAATTD  QVLDFIDKEL  GVQ 
               
            
           
         
       
     
     The amino acid sequence of  Saccharopolyspora spinosa  SpnB is: 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 10) 
                   
               
               
                         10          20          30          40          50 
                   
               
               
                 MTVTTSYEEV  VEALRASLKE  NERLRRGRDR  FSAEKDDPIA  IVAMSCRYPG 
               
               
                   
               
               
                         60          70          80          90         100 
               
               
                 QVSSPEDLWQ  LAAGGVDAIS  EVPGDRGWDL  DGVFVPDSDR  PGTSYACAGG 
               
               
                   
               
               
                        110         120         130         140         150 
               
               
                 FLQGVSEFDA  GFFGISPREA  LAMDPQQRLL  LEVAWEVFER  AGLEQRSTRG 
               
               
                   
               
               
                        160         170         180         190         200 
               
               
                 SRVGVFVGTN  GQDYASWLRT  PPPAVAGHVL  TGGAAAVLSG  RVAYSFGFEG 
               
               
                   
               
               
                        210         220         230         240         250 
               
               
                 PAVTVDTACS  SSLVALHLAG  QALRAGECDL  ALAGGVTVMS  TPKVFLEFSR 
               
               
                   
               
               
                        260         270         280         290         300 
               
               
                 QRGLAPDGRC  KSFAAGADGT  GWGEGAGLLL  LERLSDARRN  GHEVLAVVRG 
               
               
                   
               
               
                        310         320         330         340         350 
               
               
                 SAVNQDGASN  GLTAPNGSSQ  QRVITQALAS  AGLSVSDVDA  VEAHGTGTRL 
               
               
                   
               
               
                        360         370         380         390         400 
               
               
                 GDPIEAQALI  ATYGRDRDPG  RPLWLGSVKS  NIGHTQAAAG  VAGVIKMVMA 
               
               
                   
               
               
                        410         420         430         440         450 
               
               
                 MRHGQLPRTL  HVESPSPEVD  WSAGTVQLLT  ENTPWPRSGR  VRRVGVSSFG 
               
               
                   
               
               
                        460         470         480         490         500 
               
               
                 ISGTNAHVIL  EQPPGVPSQS  AGPGSGSVVD  VPVVPWMVSG  KTPEALSAQA 
               
               
                   
               
               
                        510         520         530         540         550 
               
               
                 TALMTYLDER  PDVSSLDVGY  SLALTRSALD  ERAVVLGSDR  ETLLCGVKAL 
               
               
                   
               
               
                        560         570         580         590         600 
               
               
                 SAGHEASGLV  TGSVGAGGRI  GFVFSGQGGQ  WLGMGRGLYR  AFPVFAAAFD 
               
               
                   
               
               
                        610         620         630         640         650 
               
               
                 EACAELDAHL  GQEIGVREVV  SGSDAQLLDR  TLWAQSGLFA  LQVGLLKLLD 
               
               
                   
               
               
                        660         670         680         690         700 
               
               
                 SWGVRPSVVL  GHSVGELAAA  FAAGVVSLSG  AARLVAGRAR  LMQALPSGGG 
               
               
                   
               
               
                        710         720         730         740         750 
               
               
                 MLAVPAGEEL  LWSLLADQGD  RVGIAAVNAA  GSVVLSGDRD  VLDDLAGRLD 
               
               
                   
               
               
                        760         770         780         790         800 
               
               
                 GQGIRSRWLR  VSHAFHSYRM  DPMLAEFAEL  ARTVDYRRCE  VPIVSTLTGD 
               
               
                   
               
               
                        810         820         830         840         850 
               
               
                 LDDAGRMSGP  DYWVRQVREP  VRFADGVQAL  VEHDVATVVE  LGPDGALSAL 
               
               
                   
               
               
                        860         870         880         890         900 
               
               
                 IQECVAASDH  AGRLSAVPAM  RRNQDEAQKV  MTALAHVHVR  GGAVDWRSFF 
               
               
                   
               
               
                        910         920         930         940         950 
               
               
                 AGTGAKQIEL  PTYAFQRQRY  WLVPSDSGDV  TGAGLAGAEH  PLLGAVVPVA 
               
               
                   
               
               
                        960         970         980         990        1000 
               
               
                 GGDEVLLTGR  ISVRTHPWLA  EHRVLGEVIV  AGTALLEIAL  HAGERLGCER 
               
               
                   
               
               
                       1010        1020        1030        1040        1050 
               
               
                 VEELTLEAPL  VLPERGAIQV  QLRVGAPENS  GRRPMALYSR  PEGAAEHDWT 
               
               
                   
               
               
                       1060        1070        1080        1090        1100 
               
               
                 RHATGRLAPG  RGEAAGDLAD  WPAPGALPVD  LDEFYRDLAE  LGLEYGPIFQ 
               
               
                   
               
               
                       1110        1120        1130        1140        1150 
               
               
                 GLKAAWRQGD  EVYAEAALPG  TEDSGFGVHP  ALLDAALHAT  AVRDMDDARL 
               
               
                   
               
               
                       1160        1170        1180        1190        1200 
               
               
                 PFQWEGVSLH  AKAAPALRVR  VVPAGDDAKS  LLVCDGTGRP  VISVDRLVLR 
               
               
                   
               
               
                       1210        1220        1230        1240        1250 
               
               
                 SAAARRTGAR  RQAHQARLYR  LSWPTVQLPT  SAQPPSCVLL  GTSEVSADIQ 
               
               
                   
               
               
                       1260        1270        1280        1290        1300 
               
               
                 VYPDLRSLTA  ALDAGAEPPG  VVIAPTPPGG  GRTADVRETT  RHALDLVQGW 
               
               
                   
               
               
                       1310        1320        1330        1340        1350 
               
               
                 LSDQRLNESR  LLLVTQGAVA  VEPGEPVTDL  AQAALWGLLR  STQTEHPDRF 
               
               
                   
               
               
                       1360        1370        1380        1390        1400 
               
               
                 VLVDVPEPAQ  LLPALPGVLA  CGEPQLALRR  GGAHAPRLAG  LGSDDVLPVP 
               
               
                   
               
               
                       1410        1420        1430        1440        1450 
               
               
                 DGTGWRLEAT  RPGSLDGLAL  VDEPTATAPL  GDGEVRIAMR  AAGVNFRDAL 
               
               
                   
               
               
                       1460        1470        1480        1490        1500 
               
               
                 IALGMYPGVA  SLGSEGAGVV  VETGPGVTGL  APGDRVMGMI  PKAFGPLAVA 
               
               
                   
               
               
                       1510        1520        1530        1540        1550 
               
               
                 DHRMVTRIPA  GWSFARAASV  PIVFLTAYYA  LVDLAGLRPG  ESLLVHSAAG 
               
               
                   
               
               
                       1560        1570        1580        1590        1600 
               
               
                 GVGMAAIQLA  RHLGAEVYAT  ASEDKWQAVE  LSREHLASSR  TCDFEQQFLG 
               
               
                   
               
               
                       1610        1620        1630        1640        1650 
               
               
                 ATGGRGVDVV  LNSLAGEFAD  ASLRMLPRGG  RFLELGKTDV  RDPVEVADAH 
               
               
                   
               
               
                       1660        1670        1680        1690        1700 
               
               
                 PGVSYQAFDT  VEAGPQRIGE  MLHELVELFE  GRVLEPLPVT  AWDVRQAPEA 
               
               
                   
               
               
                       1710        1720        1730        1740        1750 
               
               
                 LRHLSQARHV  GKLVLTMPPV  WDAAGTVLVT  GGTGALGAEV  ARHLVIERGV 
               
               
                   
               
               
                       1760        1770        1780        1790        1800 
               
               
                 RNLVLVSRRG  PAASGAAELV  AQLTAYGAEV  SLQACDVADR  ETLAKVLASI 
               
               
                   
               
               
                       1810        1820        1830        1840        1850 
               
               
                 PDEHPLTAVV  HAAGVLDDGV  SESLTVERLD  QVLRPKVDGA  RNLLELIDPD 
               
               
                   
               
               
                       1860        1870        1880        1890        1900 
               
               
                 VALVLFSSVS  GVLGSGGQGN  YAAANSFLDA  LAQQRQSRGL  PTRSLAWGPW 
               
               
                   
               
               
                       1910        1920        1930        1940        1950 
               
               
                 AEHGMASTLR  EAEQDRLARS  GLLPISTEEG  LSQFDAACGG  AHTVVAPVRF 
               
               
                   
               
               
                       1960        1970        1980        1990        2000 
               
               
                 SRLSDGNAIK  FSVLQGLVGP  HRVNKAATAD  DAESLRKRLG  RLPDAEQHRI 
               
               
                   
               
               
                       2010        2020        2030        2040        2050 
               
               
                 LLDLVRMHVA  AVLGFAGSQE  ITADGTFKVL  GFDSLTVVEL  RNRINGATGL 
               
               
                   
               
               
                       2060        2070        2080        2090        2100 
               
               
                 RLPATLVFNY  PTPDALAAHL  VTALSADRLA  GTFEELDRWA  ANLPTLARDE 
               
               
                   
               
               
                       2110        2120        2130        2140        2150 
               
               
                 ATRAQITTRL  QAILQSLADV  SGGTGGGSVP  DRLRSATDDE  LFQLLDNDLE 
               
               
                   
               
               
                 LP 
               
            
           
         
       
     
     The present invention provides for a polyketide synthase (PKS) capable of synthesizing a carboxylic acid or diacid. The PKS is not a naturally occurring PKS. In some embodiments, the carboxylic acid or diacid is not a compound synthesized by a naturally occurring PKS. In some embodiments, the PKS is a hybrid PKS comprising modules, domains, and/or portions thereof from two or more PKSs. Such carboxylic acids or diacids include the diketides and triketides, and polyketides of more than three ketide units, such as 4, 5, or 6 or more ketide units. The carboxylic acid or diacid can further include one or more functional groups. Such functional groups include, but are not limited to, ethyl, methyl and hydroxyl side chains, and internal olefins and ketones. 
     In some embodiments, the diacid is adipic acid (or hexanedioc acid), suberic acid (or octanedioc acid), or sebacic acid (or decanedioc acid). In some embodiments, the diacid is a symmetrical compound, such as a fully reduced symmetrical aliphatic compound. 
     Adipic acid is a six carbon chain fully reduced symmetrical aliphatic compound with no side chains, hence no chiral centers. Side chains (methyl, allyl, hydroxyl) of the carboxylic acid or diacid may be incorporated or formed, depending on the modules employed. 
     Complex polyketides comprise a large class of natural products that are synthesized in bacteria (mainly members actinomycete family; e.g.  Streptomyces ), fungi and plants. Polyketides form the aglycone component of a large number of clinically important drugs, such as antibiotics (e.g. erythromycin, tylosin), antifungal agents (e.g. nystatin), anticancer agents (e.g. epothilone), immunosuppressives (e.g. rapamycin), etc. Though these compounds do not resemble each other either in their structure or their mode of action, they share a common basis for their biosynthesis, which is carried out by a group of enzymes designated polyketide synthases. 
     Polyketide synthases (PKS) employ short chain fatty acyl CoAs in Claisen condensation reactions to produce polyketides. Unlike fatty acid synthases which utilize acetyl CoA as the starter and malonyl CoA as the extender units, and use a single module iteratively to produce the nascent acyl chains, PKSs are composed of discrete modules, each catalyzing the chain growth of a single step. Modules can differ from each other in composition so that overall, a number of different starters (e.g. acetyl CoA, propionyl CoA) and extenders, some of which contain stereospecific methyl (or ethyl) side chains can be incorporated. In addition, PKS modules do not always reduce the 3-carbonyl formed from condensation but may leave it either unreduced (ketone), partially reduced (hydroxyl, 2,3-ene) or fully reduced (3-methylene). In some cases the terminal carboxyl group is usually removed by a decarboxylase domain present at the N-terminus of the corresponding loading domain of the PKS. Because of the correspondence between use of modules in the synthesis and the structure of the polyketide produced, it is possible to program the synthesis to produce a compound of desired structure by selection and genetic manipulation of polyketide synthases.  FIG. 9  shows a scheme for making novel polyamides or novel polyesters using diacids (using adipic acid as an example).  FIG. 11  shows the various modules and the precursor utilized by each module for incorporation into the corresponding nascent acyl (polyketide) chain to give rise to the range of compounds of interest. Table 4 provides a PKS source for each module. Each PKS source is well-known to one skilled in the art is readily available. In addition, for each module taught in Table 4, there may be other modules from other PKS that can be used. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 PKS sources of the various modules. 
               
            
           
           
               
               
            
               
                 Module 
                 PKS Source 
               
               
                   
               
               
                 A 
                 Rifamycin PKS Module 2 
               
               
                 B 
                 Oligomycin PKS Module 1 
               
               
                 C 
                 Spiramycin PKS Module 1 
               
               
                 D 
                 Pikromycin PKS Module 2 
               
               
                 E 
                 Oligomycin PKS Module 3 
               
               
                 F 
                 Erythromycin PKS Module 3 
               
               
                 G 
                 Oligomycin PKS Module 5 
               
               
                 H 
                 Primaricin PKS Module 7 
               
               
                 I 
                 Tylosin PKS Module 1 
               
               
                 J 
                 Erythromycin PKS Module 1 
               
               
                 K 
                 Avermectin PKS Module 7 
               
               
                 L 
                 Rapamycin PKS Module 1 
               
               
                 M 
                 Erythromycin PKS Module 4 
               
               
                 N 
                 Pederin Module 2 
               
               
                 O 
                 Ascomycin Module 4 
               
               
                 P 
                 FK506 Module 4 
               
               
                   
               
            
           
         
       
     
     All extender modules carry the β-acyl ACP synthase (commonly called the ketosynthase or KS) domain, which conducts the decarboxylative condensation step between the extender and the growing polyketide chain, and the acyl carrier protein (ACP) domain that carries the growing acyl chain and presents it to the cognate reductive domains for reduction of the β-carbonyl. Modules can differ from each other in composition so that a number of different starter and extender units, some of which contain stereospecific side chains (e.g. methyl, ethyl, propylene) can be incorporated. The acyltransferase (AT) domain of each module determines the extender unit (e.g. malonyl CoA, methylmalonyl CoA, etc.) incorporated. In addition, PKS modules do not always reduce the β-carbonyl formed from condensation but may leave it either unreduced (ketone), partially reduced (hydroxyl, 2,3-ene) or fully reduced (3-methylene). The ketoreductase (KR) domain reduces the ketone to the OH function (stereospecifically); the dehydratase (DH) domain removes water from the α and β carbons leaving an α,β trans-double bond; the enoylreductase (ER) domain reduces the double bond to a β-methylene center; the reductive state of the β-carbonyl, therefore, is determined by the presence of functional reductive domains in the corresponding module. Less commonly, modules are found to contain an additional C-methylation domain (yielding an additional α-methyl side chain, as in epothilone). The makeup of the PKS, therefore, determines the choice of starter and extender acyl units incorporated, the extent of reduction at each condensation step, and the total number of units added to the chain. The wide diversity of structures of polyketides seen in nature is attributed to the diversity in PKS compositions. 
     A partial list of sources of PKS sequences that can be used in making the PKSs of the present invention, for illustration and not limitation, includes Ambruticin (U.S. Pat. No. 7,332,576); Avermectin (U.S. Pat. No. 5,252,474; MacNeil et al., 1993, Industrial Microorganisms: Basic and Applied Molecular Genetics, Baltz, Hegeman, &amp; Skatrud, eds. (ASM), pp. 245-256; MacNeil et al., 1992, Gene 115: 119-25); Candicidin (FRO008) (Hu et al., 1994, Mol. Microbiol. 14: 163-72); Epothilone (U.S. Pat. No. 6,303,342); Erythromycin (WO 93/13663; U.S. Pat. No. 5,824,513; Donadio et al., 1991, Science 252:675-79; Cortes et al., 1990, Nature 348:176-8); FK506 (Motamedi et al., 1998, Eur. J. Biochem. 256:528-34; Motamedi et al., 1997, Eur. J. Biochem. 244:74-80); FK520 or ascomycin (U.S. Pat. No. 6,503,737; see also Nielsen et al., 1991, Biochem. 30:5789-96); Jerangolid (U.S. Pat. No. 7,285,405); Leptomycin (U.S. Pat. No. 7,288,396); Lovastatin (U.S. Pat. No. 5,744,350); Nemadectin (MacNeil et al., 1993, supra); Niddamycin (Kakavas et al., 1997, J. Bacteriol. 179:7515-22); Oleandomycin (Swan et al., 1994, Mol. Gen. Genet. 242:358-62; U.S. Pat. No. 6,388,099; Olano et al., 1998, Mol. Gen. Genet. 259:299-308); Pederin (PCT publication no. WO 2003/044186); Pikromycin (Xue et al., 2000, Gene 245:203-211); Pimaricin (PCT publication no. WO 2000/077222); Platenolide (EP Pat. App. 791,656); Rapamycin (Schwecke et al., 1995, Proc. Natl. Acad. Sci. USA 92:7839-43); Aparicio et al., 1996, Gene 169:9-16); Rifamycin (August et al., 1998, Chemistry &amp; Biology, 5: 69-79); Soraphen (U.S. Pat. No. 5,716,849; Schupp et al., 1995, J. Bacteriology 177: 3673-79); Spiramycin (U.S. Pat. No. 5,098,837); Tylosin (EP 0 791,655; Kuhstoss et al., 1996, Gene 183:231-36; U.S. Pat. No. 5,876,991). Additional suitable PKS coding sequences are readily available to one skilled in the art, or remain to be discovered and characterized, but will be available to those of skill (e.g., by reference to GenBank). Each of the references cited is hereby specifically and individually incorporated by reference. 
     Of the more than thirty PKSs examined, the correspondence between use of modules in the biosynthesis and the structure of the polyketide produced is fully understood both at the level of the protein sequence of the PKS and the DNA sequence of the corresponding genes. The programming of modules into polyketide structure can be identified by sequence determination. It is possible to clone (or synthesize) DNA sequences corresponding to desired modules and transfer them as fully functioning units to heterologous, otherwise non-polyketide producing hosts such as  E. coli  (B. A. Pfeifer, et al.,  Science  291, 1790 (2001)) and  Streptomyces  (C. M. Kao, et al.,  Science  265, 509 (1994)). Additional genes employed for polyketide biosynthesis have also been identified. Genes that determine phosphopantetheine:protein transferase (PPTase) that transfer the 4-phosphopantetheine co-factor of the ACP domains, commonly present in polyketide producing hosts, have been cloned in  E. coli  and other hosts (K. J. Weissman, et al.,  Chembiochem  5, 116 (2004)). It is also possible to re-program polyketide biosynthesis to produce a compound of desired structure by either genetic manipulation of a single PKS or by construction of a hybrid PKS composed of modules from two or more sources (K. J. Weissman, et al.,  Chembiochem  5, 116 (2004)). 
     Recombinant methods for manipulating modular PKS genes to make the PKSs of the present invention are described in U.S. Pat. Nos. 5,672,491; 5,843,718; 5,830,750; 5,712,146; and 6,303,342; and in PCT publication nos. WO 98/49315 and WO 97/02358; hereby incorporated by reference. A number of genetic engineering strategies have been used with various PKSs to demonstrate that the structures of polyketides can be manipulated to produce novel polyketides (see the patent publications referenced supra and Hutchinson, 1998, Curr. Opin. Microbiol. 1:319-329, and Baltz, 1998, Trends Microbiol. 6:76-83; hereby incorporated by reference). In some embodiments, the components of the hybrid PKS are arranged onto polypeptides having interpolypeptide linkers that direct the assembly of the polypeptides into the functional PKS protein, such that it is not required that the PKS have the same arrangement of modules in the polypeptides as observed in natural PKSs. Suitable interpolypeptide linkers to join polypeptides and intrapolypeptide linkers to join modules within a polypeptide are described in PCT publication no. WO 00/47724, hereby incorporated by reference. 
     The vast number of polyketide pathways that have been elucidated provide a host of different options to produce these diacids as well as the large number of derivatives. While the products can be vastly different in size and functionality, all employ virtually the same strategy for biosynthesis. The exact interfaces between non-cognate enzyme partners will be determined on a case-by-case basis. ACP-linker-KS and ACP-linker-TE regions from the proteins of interest will be aligned to examine the least disruptive fusion point for the hybrid synthase. Genetic constructions will employ sequence and ligation independent cloning (SLIC) so as to eliminate the incorporation of genetic “scarring”. 
     Nucleic Acids Encoding the PKS 
     The present invention provides for a recombinant nucleic acid that encodes a polyketide synthase (PKS) of the present invention. The recombinant nucleic acid can be a double-stranded or single-stranded DNA, or RNA. The recombinant nucleic acid can encode an open reading frame (ORF) of the PKS of the present invention. The recombinant nucleic acid can also comprise promoter sequences for transcribing the ORF in a suitable host cell. The recombinant nucleic acid can also comprise sequences sufficient for having the recombinant nucleic acid stably replicate in a host cell. The recombinant nucleic acid can be replicon capable of stable maintenance in a host cell. In some embodiments, the replicon is stably integrated into a chromosome of the host cell. In some embodiments, the replicon is a plasmid. The present invention also provides for a vector or expression vector comprising a recombinant nucleic acid of the present invention. The present invention provides for a host cell comprising any of the recombinant nucleic acid and/or PKS of the present invention. In some embodiments, the host cell, when cultured under a suitable condition, is capable of producing the carboxylic acid or diacid. 
     It will be apparent to one of skill in the art that a variety of recombinant vectors can be utilized in the practice of aspects of the invention. As used herein, “vector” refers to polynucleotide elements that are used to introduce recombinant nucleic acid into cells for either expression or replication. Selection and use of such vehicles is routine in the art. An “expression vector” includes vectors capable of expressing DNAs that are operatively linked with regulatory sequences, such as promoter regions. Thus, an expression vector refers to a recombinant DNA or RNA construct, such as a plasmid, a phage, recombinant virus or other vector that, upon introduction into an appropriate host cell, results in expression of the cloned DNA. Appropriate expression vectors are well known to those of skill in the art and include those that are replicable in eukaryotic cells and/or prokaryotic cells and those that remain episomal or those that integrate into the host cell genome. 
     The vectors may be chosen to contain control sequences operably linked to the resulting coding sequences in a manner that expression of the coding sequences may be effected in an appropriate host. Suitable control sequences include those that function in eukaryotic and prokaryotic host cells. If the cloning vectors employed to obtain PKS genes encoding derived PKS lack control sequences for expression operably linked to the encoding nucleotide sequences, the nucleotide sequences are inserted into appropriate expression vectors. This can be done individually, or using a pool of isolated encoding nucleotide sequences, which can be inserted into host vectors, the resulting vectors transformed or transfected into host cells, and the resulting cells plated out into individual colonies. Suitable control sequences for single cell cultures of various types of organisms are well known in the art. Control systems for expression in suitable host cells, such as yeast and prokaryotic host cells, are widely available and are routinely used. Control elements include promoters, optionally containing operator sequences, and other elements depending on the nature of the host, such as ribosome binding sites. Particularly useful promoters for prokaryotic hosts include those from PKS gene clusters that result in the production of polyketides as secondary metabolites, including those from Type I or aromatic (Type II) PKS gene clusters. Examples are act promoters, tcm promoters, spiramycin promoters, and the like. However, other bacterial promoters, such as those derived from sugar metabolizing enzymes, such as galactose, lactose (lac) and maltose, are also useful. Additional examples include promoters derived from biosynthetic enzymes such as for tryptophan (trp), the β-lactamase (bla), bacteriophage lambda PL, and T5. In addition, synthetic promoters, such as the tac promoter (U.S. Pat. No. 4,551,433; hereby incorporated by reference), can be used. 
     As noted, particularly useful control sequences are those which themselves, or with suitable regulatory systems, activate expression during transition from growth to stationary phase in the vegetative mycelium. Illustrative control sequences, vectors, and host cells of these types include the modified  S. coelicolor  CH999 and vectors described in PCT publication no. WO 96/40968 and similar strains of  S. lividans . See U.S. Pat. Nos. 5,672,491; 5,830,750; 5,843,718; and 6,177,262, each of which is hereby incorporated by reference. Other regulatory sequences may also be desirable which allow for regulation of expression of the PKS sequences relative to the growth of the host cell. Regulatory sequences are known to those of skill in the art, and examples include those which cause the expression of a gene to be turned on or off in response to a chemical or physical stimulus, including the presence of a regulatory compound. Other types of regulatory elements may also be present in the vector, for example, enhancer sequences. 
     Selectable markers can also be included in the recombinant expression vectors. A variety of markers are known which are useful in selecting for transformed cell lines and generally comprise a gene whose expression confers a selectable phenotype on transformed cells when the cells are grown in an appropriate selective medium. Such markers include, for example, genes that confer antibiotic resistance or sensitivity to the plasmid. 
     The various PKS nucleotide sequences, or a mixture of such sequences, can be cloned into one or more recombinant vectors as individual cassettes, with separate control elements or under the control of a single promoter. The PKS subunits or components can include flanking restriction sites to allow for the easy deletion and insertion of other PKS subunits. The design of such restriction sites is known to those of skill in the art and can be accomplished using the techniques described above, such as site-directed mutagenesis and PCR. Methods for introducing the recombinant vectors of the present invention into suitable hosts are known to those of skill in the art and typically include the use of CaCl 2  or other agents, such as divalent cations, lipofection, DMSO, protoplast transformation, conjugation, and electroporation. 
     Host Cells Comprising the PKS 
     The present invention provides for a host cell comprising any of the recombinant nucleic acid and/or PKS of the present invention. In some embodiments, the host cell, when cultured, is capable of producing a carboxylic acid or diacid. The host cell can be a eukaryotic or a prokaryotic cell. In some embodiments, the host cell is a non-human cell. Suitable eukaryotic cells include yeast cells, such as from the genus  Saccharomyces  or  Schizosaccharomyces . A suitable species from the genus  Saccharomyces  is  Saccharomyces cerevisiae . A suitable species from the genus  Schizosaccharomyces  is  Schizosaccharomyces pombe . Suitable prokaryotic cells include  Escherichia coli  or  Streptomyces  species. 
     The PKS can be in a host cell, or isolated or purified. The PKS can synthesize the carboxylic acid or diacid in vivo (in a host cell) or in vitro (in a cell extract or where all necessary chemical components or starting materials are provided). The present invention provides methods of producing the carboxylic acid or diacid using any of these in vivo or in vitro means. 
     Methods of Using the PKS 
     The present invention provides a method of producing a carboxylic acid or diacid, comprising: providing a host cell of the present invention, and culturing said host cell in a suitable culture medium such that the carboxylic acid or diacid is produced. The method can further comprise isolating said carboxylic acid or diacid from the host cell and the culture medium. The method can further comprise reacting the diacid with a diamine to produce a nylon. A suitable diamine is an alkane diamine, such as hexane-1,6-diamine. Alternatively, the method can further comprise reacting the diacid with a dialcohol to produce a polyester. A suitable dialcohol is an alkane diol, such as ethylene glycol, propane diol, or butanediol. A variety of methods for heterologous expression of PKS genes and host cells suitable for expression of these genes and production of polyketides are described, for example, in U.S. Pat. Nos. 5,843,718; 5,830,750 and 6,262,340; WO 01/31035, WO 01/27306, and WO 02/068613; and U.S. Patent Application Pub. Nos. 2002/0192767 and 2002/0045220; hereby incorporated by reference. 
     The present invention provides for a composition comprising a carboxylic acid or diacid isolated from a host cell from which the carboxylic acid or diacid is produced, and trace residues and/or contaminants of the host cell. Such trace residues and/or contaminants include cellular material produced by the lysis of the host cell. 
     The diacids, such as adipic acid, provide for the production of “green” nylon, such as that used in Mohawk carpet fibers. Besides nylon production, the ability to manipulate the side chains of the diacids provides for the production of novel polymer precursors that would lead to polymers with a variety of properties. These products may also serve as adhesive, lubricants or precursors for pharmaceuticals or other more complicated compounds. 
     The present invention has one or more of the following advantages: (1) it reduces the dependence on oil for producing certain chemicals, and (2) it serves as a means of capture and sequestration of carbon from the atmosphere. 
     REFERENCES CITED 
     
         
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         Hagen, A., Poust, S., de Rond, T., Yuzawa, S., Katz, L., Adams, P. D., Petzold, C. J., and Keasling, J. D. (2014). In Vitro Analysis of Carboxyacyl Substrate Tolerance in the Loading and First Extension Modules of Borrelidin Polyketide Synthase. Biochemistry (Mosc.) 53, 5975-5977. 
         Hong, H., Appleyard, A. N., Siskos, A. P., Garcia-Bernardo, J., Staunton, J., and Leadlay, P. F. (2005). Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry: Limited proteolysis and MS of modular PKSs. FEBS J. 272, 2373-2387. 
         Kellenberger, L., Galloway, I. S., Sauter, G., Bihm, G., Hanefeld, U., Cortés, J., Staunton, J., and Leadlay, P. F. (2008). A Polylinker Approach to Reductive Loop Swaps in Modular Polyketide Synthases. ChemBioChem 9, 2740-2749. 
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         Meluzzi, D., Zheng, W. H., Hensler, M., Nizet, V., and Dorrestein, P. C. (2008). Top-down mass spectrometry on low-resolution instruments: characterization of phosphopantetheinylated carrier domains in polyketide and non-ribosomal biosynthetic pathways. Bioorg. Med. Chem. Lett. 18, 3107-3111. 
         Tang, Y., Kim, C.-Y., Mathews, I. I., Cane, D. E., and Khosla, C. (2006). The 2.7-A crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase. Proc. Natl. Acad. Sci. 103, 11124-11129. 
         Vergnolle, O., Hahn, F., Baerga-Ortiz, A., Leadlay, P. F., and Andexer, J. N. (2011). Stereoselectivity of Isolated Dehydratase Domains of the Borrelidin Polyketide Synthase: Implications for cis Double Bond Formation. ChemBioChem 12, 1011-1014. 
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         Yoon, Y. J., Beck, B. J., Kim, B. S., Kang, H.-Y., Reynolds, K. A., and Sherman, D. H. (2002). Generation of Multiple Bioactive Macrolides by Hybrid Modular Polyketide Synthases in  Streptomyces venezuelae . Chem. Biol. 9, 203-214. 
         Yu, J.-L., Xia, X.-X., Zhong, J.-J., and Qian, Z.-G. (2014). Direct biosynthesis of adipic acid from a synthetic pathway in recombinant  Escherichia coli : Adipic Acid Production From a Synthetic Pathway. Biotechnol. Bioeng. 111, 2580-2586. 
         Zheng, J., Piasecki, S. K., and Keatinge-Clay, A. T. (2013). Structural Studies of an A2-Type Modular Polyketide Synthase Ketoreductase Reveal Features Controlling α-Substituent Stereochemistry. ACS Chem. Biol. 8, 1964-1971. 
       
    
     The invention having been described, the following examples are offered to illustrate the subject invention by way of illustration, not by way of limitation. 
     Example 1 
     Engineering a Polyketide Synthase for Production of Adipic Acid 
     Polyketides have enormous structural diversity, yet polyketide synthases (PKSs) have thus far been engineered to produce only derivatives of drugs or drug candidates. Thousands of other molecules, including commodity and specialty chemicals could be synthesized using PKSs if composing hybrid PKSs from well-characterized parts derived from natural PKSs was more efficient. Here, using modern proteomics techniques, we demonstrate construction of a chimeric PKS extension module capable of producing one of the most widely used commodity chemicals, adipic acid. To accomplish this, we introduced heterologous reductive domains from various PKS clusters into the borrelidin PKS&#39; first extension module, which we previously showed produces a 3-hydroxy-adipoyl intermediate when co-incubated with the loading module and a succinyl-CoA starter unit. Acyl-ACP intermediate analysis revealed an unexpected bottleneck at the dehydration step which was overcome by introduction of a carboxyacyl-processing dehydratase domain from the second module of the borrelidin. Adipic acid was released from the synthase after appending the erythromycin thioesterase domain to the hybrid PKS. 
     The results demonstrate the following: 
     1. Demonstration of commodity chemical production by an engineered polyketide synthase. 
     2. Acyl-ACP intermediate analysis is used to identify unexpected catalytic bottlenecks. 
     3. Identification of previously unknown dehydratase domain selectivity. 
     4. Construction of a broad specificity, fully-reducing PKS module. 
     Using proteomics-based covalent intermediate analysis, Hagen et al engineered a chimeric polyketide synthase capable of producing adipic acid. In the process they revealed unexpected selectivity in the β-carbon reduction cycle. 
     Introduction 
     Here we demonstrate engineering a PKS to produce the commodity chemical adipic acid. Current production of adipic acid results in approximately 10% of anthropogenic emissions of N 2 O—a potent greenhouse gas (Alini et al., 2007)); therefore, a biological route to adipic acid could be an important alternative. 
     Within the context of type I PKS-based biosynthesis, we proposed that adipic acid synthesis would most conveniently start from the four-carbon succinyl-CoA, undergo one round of extension with full reduction using a malonyl-CoA extender unit to produce the six carbon adipoyl-ACP intermediate. Adipic acid would then be released from adipoyl-ACP by the action of a thioesterase. Due to its important role in the TCA cycle, succinate/succinyl-CoA is readily available in organisms capable of aerobic respiration (e.g. common production hosts like  E. coli, Saccharomyces cerevisiae  and Actinobacteria), as is malonyl-CoA, which is used in fatty acid biosynthesis. Therefore production of adipic acid using a PKS and succinyl-CoA starter would be relatively host and feedstock agnostic, as minimal metabolic engineering would be necessary to ensure adequate precursor supply. Another advantage of using a PKS system is the extensibility inherent in its modular nature. For example, longer diacids could be generated by use of additional (or iterative) modules, and novel adipic acid analogs could be created with α-substitutions (e.g. methyl-, fluoro-, or allyl groups) that may yield polymers with useful attributes such as cross-linkable chemical handles (Epstein, 1979). 
     Previous work in our lab demonstrated that the loading and first extension modules of the borrelidin PKS (hereafter referred to as “BorLM” and “BorMod1”, respectively) are capable of producing a 3-hydroxy-adipoyl-ACP intermediate in vitro using succinyl-CoA as a starter substrate and the natural extender substrate, malonyl-CoA (Hagen et al., 2014) ( FIG. 1B ). To proceed from the 3-hydroxyadipoyl-ACP intermediate to adipic acid, additional β-carbonyl processing and hydrolytic chain release is required. We therefore sought to introduce additional reducing domains into BorMod1, and upon verification of complete reduction, append a thioesterase domain capable of releasing the linear product. “Reductive loop” swaps were among the earliest and most successful demonstrations of modularity in type I PKS systems (Donadio et al., 1993; Gaisser et al., 2003; McDaniel et al., 1999; Yoon et al., 2002). These findings along with limited proteolysis experiments and recent structural studies indicate that reductive loops function as integral units (Aparicio et al., 1994; Dutta et al., 2014; Hong et al., 2005). Despite these examples, no prescriptive rules have been developed to guide successful reductive loop swaps and the most extensive, combinatorial study of reductive loop swaps to date ultimately concluded, “no single donor [module] and no single pair of splice sites were found to be reliably optimal to effect a given alteration” (Kellenberger et al., 2008). 
     We selected donor reductive loops from the aureothin, indanomycin, nanchangmycin and spinosyn PKS clusters: AurB, IdmO, NanA2, SpnB, respectively, based on three criteria: (1) the loop contained the full complement of reducing domains (ketoreductase, dehydratase and enoyl reductase, hereafter referred to as “KR,” “DH,” and “ER,” respectively), (2) the loop originated from a “standalone” module in which the open reading frame or “subunit” encodes just a single module, and (3) the module harboring the reductive loop naturally incorporates a malonate extender unit. Previous work has suggested a reduction in catalytic efficiency and relaxed stereoselectivity when KR domains are presented with an α-carbon differentially substituted than the KR&#39;s normal substrate (McDaniel et al., 1999; Zheng et al., 2013). These loops were introduced combinatorially into BorMod1 using two alternative N-terminal and a single C-terminal splice sites to generate eight chimeras to be tested for adipoyl-ACP production in vitro ( FIG. 1A ). 
     In the absence of a thioesterase, intermediates covalently attached to the PKS could be monitored using the “PPant ejection assay” (Meluzzi et al., 2008). This system allows us to identify bottlenecks in the biosynthesis. As PKSs are complex enzymes, determining the point of failure for engineered PKSs is challenging. Most PKS engineering efforts thus far have relied on the presence of the desired final product to determine success, however this approach does not provide information as to where the enzymatic assembly line has stalled if the product is not observed. As part of our efforts to produce the commodity chemical adipic acid, we demonstrate the utility of acyl-carrier protein (ACP) intermediate analysis (via the PPant ejection assay) to “debug” PKSs. Upon satisfactory production of adipoyl-ACP after several rounds of chimeragenesis, a thioesterase was introduced to produce adipic acid. 
     Results 
     Beta-Carbonyl Processing Stalls at the Dehydration Step; is Alleviated by Provision of BorDH2 in Trans 
     The initial engineered reductive loop BorMod1 library was incubated with the synthetic starter substrate succinyl-SNAC, along with malonyl-CoA and NADPH. Six out of eight constructs were catalytically active, but the primary acyl-ACP species, after introduction of the full reducing loop, remained the partially reduced 3-hydroxy-adipoyl-ACP intermediate; the 3-keto, 2,3-ene and fully reduced (adipoyl-ACP) products were not detected (see  FIG. 2A ), indicating that reductive processing was stalled at the dehydratase step. 
     We hypothesized the dehydratase domains from the reductive loop variants were not competent to dehydrate 3-hydroxyadipoyl-ACP and therefore sought to test the activity of a different dehydratase domain which processes a substrate carrying a terminal carboxyl group in its natural context. Because of its proximity to a terminal carboxyl group (see  FIG. 1B ), the first DH domain in the borrelidin cluster, BorDH2, was chosen and provided to the reductive loop library in trans in stoichiometric excess as previous work showed a low rate of DH activity in vitro (Vergnolle et al., 2011). As shown in  FIG. 2B , provision of BorDH2 resulted in the production of higher levels of the adipoyl-ACP intermediate when compared to the constructs without BorDH2. A particularly interesting case is the comparison between S2 and S2t, where provision of the dehydratase in trans (S2t), increased adipoyl-ACP production from nearly undetectable levels to the highest level amongst all variants. No significant accumulation of the 2,3-ene-ACP intermediate was observed when BorDH2 was provided (data not shown). This, along with the observed production of adipoyl-ACP in all loop variants, indicates the 2,3-ene intermediate, the immediate product of the dehydration, was readily reduced by the enoyl reductase domains present in cis. 
       FIG. 6  shows the N- and C-terminal junctions for initial constructs. Arrows indicates crossover point.  FIG. 7  shows the junctions for DH swap constructs. Arrows indicates crossover point.  FIG. 8  shows the N-terminal junctions including junction 3. 
     BorDH2 in Cis Further Increases the Proportion of Adipoyl-ACP 
     Having demonstrated that BorDH2 provided in trans is capable of promoting adipoyl-ACP formation, we next asked whether this was a property unique to this particular dehydratase domain or simply because BorDH2 was provided in stoichiometric excess. To determine this, BorDH2 was swapped into a subset of the most active reductive loop library members in order to replace the native DH domain. After purification, these DH swapped variants were compared to previous constructs as before via intermediate analysis after extension of succinyl-SNAC. 
     As shown in  FIG. 2C , DH swapped variants clearly promoted the formation of adipoyl-ACP (e.g. compare A2 ( FIG. 2A ) to A2c ( FIG. 2C )) at levels comparable to where the DH was provided in trans at 50-fold stoichiometric excess (e.g. compare A2t ( FIG. 2B ) to A2c ( FIG. 2C )). These data demonstrate that it is the unique identity of the BorDH2 domain which allows β-carbonyl processing and which is not required at stoichiometric excess for maximum activity. 
     Refined Chimeric Junction Further Promotes Proportion of Adipoyl-ACP 
     Despite junction 2 PKS variants generally showing higher production of adipoyl-ACP than junction 1 variants (especially when BorDH2 was included in cis), further sequence and structural analysis indicated that junction 2 constructs may be truncated by approximately 15 residues (depending on how domain boundaries are annotated) at the N-terminus of the dehydratase domain (see supplemental information). These residues are distal to the active site and ACP docking interface and are clearly not essential, however their influence on the overall tertiary structure and kinetics of PKS enzymes was unclear. Therefore, a new N-terminal junction was selected intermediary to junctions 1 and 2 (junction 3). Variants were created for a subset of the reductive loop library which included the best performing AurB and SpnB loop sources both with and without the BorDH2 swap. This location immediately follows the post-AT linker region which is believed to be important for proper KS-AT domain orientation (Tang et al., 2006) and also restores the missing segment in the DH domain N-terminal truncations. 
     As shown in  FIG. 2D , junction 3 was found to be superior to junction 1 and junction 2 as gauged by total production of the adipoyl-ACP intermediate. Strikingly, the combination of the new junction with the BorDH2 swap displayed a synergistic effect as evidenced by the nearly complete intermediate conversion to adipoyl-ACP in the case of A3c and S3c constructs. 
     BorDH2 is Necessary Solely for Carboxy-Acyl Processing 
     The aforementioned data suggest that dehydration of a carboxyacyl-ACP intermediate is a trait unique to BorDH2 and not shared by the four DH domains in the un-engineered reductive loops. The possibility, however, remains that the ACP in BorMod1 does not interact well with non-native DH domains, precluding the presentation of the 3-hydroxy-adipoyl-ACP intermediate, whereas the ACP more readily associates with a DH domain from the same PKS cluster. To interrogate this possibility, we incubated the isolated ACP monodomain from BorLM (which naturally presents loaded substrates to BorMod1,  FIG. 1B ) acylated with a variety of carboxy and descarboxy-CoA substrates with BorDH2-swapped and unswapped version of the S3 variant to determine which substrates could be processed. The CoAs employed were succinyl-CoA and its descarboxy analog propionyl-CoA as well as the natural substrate 1,2-cyclopentanedicarboxyl-CoA (CPDA-CoA) and its respective descarboxy analog cyclopentanemonocarboxyl-CoA (CPMA-CoA). As shown in  FIGS. 3 and 5 , the descarboxy substrates propionyl- and CPMA-ACP were extended and fully reduced to their respective products by both S3 and S3c protein variants. In contrast, only the BorDH2 swapped variant converted a significant fraction of the 3-hydroxy intermediates to the fully reduced species when carboxylated substrates were provided. These results demonstrate unambiguously that the un-engineered reductive loop of SpnB is competent to perform full β-carbonyl processing of the more typical non-carboxylated intermediates, however BorDH2 is required for full β-carbonyl processing when the substrate contains a distal carboxy group. 
     Appending a Thioesterase Domain Allows for Production of Free Adipic Acid 
     Having demonstrated the construction of a highly engineered extension module capable of producing adipoyl-ACP, we next sought to produce free adipic acid by the addition of a thioesterase (TE). The well-characterized TE domain from the erythromycin cluster was therefore appended to the best performing S3c variant in place of the C-terminal docking domain to create S3c-TE. In order to compare the activity and product profile of the engineered extension module with that of the wild type module, the TE was also appended to wild type BorMod1 to create BorMod1-TE. The proteins were purified and extension assays performed as before and titers were measured via LC-MS/MS by comparison to authentic standards (see materials and methods for synthesis of 3-hydroxy-adipic acid).  FIG. 4  shows that as expected, the BorMod1-TE construct produced exclusively 3-hydroxy-adipic acid whereas S3c-TE produced a mixture of the partially and fully reduced adipic acid products. While titers are modest, it is noteable that the titers for the wildtype and engineered extension modules are similar. This would suggest that despite the introduction of five chimeric junctions and utilization of domains from three different PKS clusters, the overall kinetics of the engineered extension module are comparable to wildtype. 
     Discussion 
     In this study, using proteomics-based intermediate analysis to inform design iterations, we have demonstrated, for the first time, production of a commodity chemical by an engineered polyketide synthase. This was facilitated by prior identification of an extension module, BorMod1, which naturally accepts carboxyacyl substrates and extends with malonyl-CoA. Metabolomic analysis of intermediates in solution has been utilized for bottleneck determination and subsequent improvement of engineered pathways (George et al., 2014). Here, by analysis of the covalent intermediates on the PKS assembly line, we have demonstrated the utility of this methodology to pinpoint and alleviate unexpected catalytic bottlenecks. 
     Initial activity tests indicated that replacing the reductive loop from BorMod1 with a library of reductive loops from fully reducing modules does not compromise the catalytic competence of the module for the extension reaction. This lends further support to the idea that the reductive loop functions as an “integral unit” apart from the core catalytic activity of the acyltransferase and ketosynthase domains in the module, and that the chimeric junctions used in this study did not perturb the module&#39;s tertiary structure such that condensation is precluded. 
     Interestingly, intermediate analysis showed that dehydration activity on carboxylated 3-hydroxy-ACP intermediates was poor, whereas β-carbonyl processing proceeded uninterrupted using descarboxy substrate analogs, revealing a previously unknown biochemical incompatibility between carboxylated substrates and typical dehydratase domains. In contrast, BorDH2 which in its native context processes a carboxylated substrate, appears substrate agnostic, though more kinetic data would be required to determine whether it prefers one species over the other. It is interesting to note that BorDH2 normally processes a cyclic intermediate with a sterically constrained carboxy group at the 8 position (see  FIG. 1B ), rather than a linear 6-carboxy intermediate. Future bioinformatic and structural studies could reveal structural determinants of diacid tolerance and could enable engineering of diacid tolerance into typical reductive loops using precise amino acid substitutions of the dehydratase domain rather than chimeric domain swaps. 
     Addition of a thioesterase to the S3c PKS variant enabled production of free acids. Attenuating the TE activity or tuning its specificity towards the fully reduced product through mutagenesis could possibly shift the product profile further towards adipic acid. Alternatively, increasing the rate of β-carbonyl processing, perhaps through further refined chimeric boundary sampling (including at the C-terminus of the reductive loop) or selection of alternative reductive loops, would increase the proportion and possibly titer of adipic acid. Encouragingly, the overall activities of the wildtype BorMod1-TE and S3c-TE are within error indicating that despite extensive reductive loop engineering, the kinetics of the engineered PKS module was not significantly compromised. Further engineering of hosts for improved expression of heterologous PKSs will be required to improve the productivity of these enzymes. 
     In recent years a number of biological routes to adipic acid have been developed, typically dependent on reversal of beta-oxidation of dicarboxylic acids (Yu et al., 2014) or omega-oxidation of fatty acids (Clomburg et al., 2015). However, as demonstrated here, the ability to engineer diacid tolerance in a PKS system sets the stage for production of other valuable commodity chemicals (e.g. the eight carbon suberic acid) as well as branched diacids that are not readily accessible through conventional synthetic chemistry or the above biosynthetic routes. 
     Significance 
     Polyketide synthases have tremendous synthetic potential, yet have historically been used only for the production of drugs and their derivatives. We show PKSs can also be used for the production of commodity chemicals by engineering a PKS that produces adipic acid. In so doing, we have highlighted the utility of LC-MS/MS based acyl-intermediate analysis techniques which allowed for identification and alleviation of the dehydratase catalytic bottleneck and revealed an unexpected biochemical incompatibility between typical dehydratase domains and carboxylated intermediates. As type I PKSs are inherently modular, this work sets the stage for production of other valuable commodity chemicals such as branched diacids which are not readily accessible through conventional synthetic chemistry. 
     Experimental Procedures 
     For details of plasmid construction, protein purifications, chemical syntheses and LC-MS/MS methods, refer to supplemental information. 
     Intermediate Analysis of PKS Variants 
     For extensions with succinyl-SNAC, a master mix (final concentrations: 1 mM succinyl-SNAC, 0.2 mM malonyl-CoA, 1 mM NADPH, 2.5 mM TCEP in 100 mM phosphate buffer pH 6.8) was aliquoted to separate tubes, to which 5 uM final concentration of each respective PKS variant was added. For relevant experiments, 50 μM BorDH2 was provided in trans. For extensions using acyl-ACP reagents, ACPs were expressed in apo form and charged using Sfp and various acyl-CoAs as described in (Hagen et al., 2014). These were added to enzyme mixes containing either the S3 or S3c PKS variants and other reaction components at the same concentration as described above. Reactions were incubated at room temperature overnight (˜16 hr). Samples were digested with 1:20 w/w porcine trypsin (Sigma-Aldrich) for 4-6 hours at 37 C prior to LC-MS/MS analysis. 
     Product Analysis of Thioesterase-Harboring Constructs 
     50 μl reactions were set up as described in intermediate analysis except the final concentration of malonyl-CoA was 0.5 mM. After incubation, samples were diluted with one volume of LC-MS grade water and filtered through 3K molecular weight cut off spin filters (Amicon) which were washed prior to use by filtration of 500 μl of LC-MS grade water. Samples were acidified by the addition of 1% formic acid prior to LC-MS/MS analysis. A dilution series of (3-hydroxy) adipic acid authentic standards was created and processed identically in parallel with samples to generate a concentration standard curve for quantification. 
     Experimental Procedures 
     Plasmid construction. Reductive loops were codon-optimized for  E. coli  and introduced into pARH100 (Hagen et al., 2014) via scarless Gibson assembly (see below for junction boundaries). The j5 algorithm and Device Editor graphical user interface were used to design oligonucleotides and DNA assembly strategies (Hillson et al., 2012). 
     Purification of PKS constructs. Plasmids were introduced into  E. coli  strain BAP1 (Pfeifer, 2001) and cultures (IL) were grown at 37° C. in terrific broth to an O.D. of approximately 1.0 and then 60 ng/ml anhydrotetracycline and 200 uM isopropyl-β-D-galactopyranoside (IPTG) were added to induce expression of PKS proteins and Sfp, respectively. Cultures continued incubation at 18 C for 20 hours after which cells were pelleted and stored at −20 C until further processing. Pellets were resuspended in lysis buffer (300 mM NaCl, 50 mM sodium phosphate, pH 6.8, 10 mM imidazole) supplemented with 0.1 mg/ml lysozyme. Suspensions were lysed by several passages through an EmulsiFlex C3 homogenizer (Avestin) and cellular debris was removed by centrifugation (15000 g, 30 minutes). Cobalt resin (2-3 ml) was added to the supernatant and mixed at 4 C for one hour before being applied to a fritted column. Resin was washed with lysozyme-free lysis buffer until flow-through resulted in no color change when mixed with Bradford reagent. Proteins were eluted with several resin volumes of elution buffer (300 mM NaCl, 50 mM phosphate, pH 6.8, 200 mM imidazole) and concentrated via spin filtration (Amicon, 100 kDa MWCO). Concentrated eluate was exchanged into storage buffer (50 mM phosphate, pH 6.8, 10% glycerol) using a PD-10 column (GE Life Sciences), and then further concentrated prior to being flash frozen in liquid nitrogen and stored at −80 C. 
     Purification of BorDH2. BorDH2 monodomain was purified as above with the exception that protein was concentrated with a 10 kDa MWCO filter and stored as a 50% glycerol solution at −20 C after buffer exchange. 
     Reagents and Chemicals. HisPur cobalt resin was purchased from Thermo Scientific, Bradford reagent was from Bio-Rad and SDS-PAGE gels from Life Technologies. 
     Chemical synthesis and NMR data. Solvents (hexanes, ethyl acetate, dichloromethane and methanol) were purchased from EDH; trans-β-hydromuconic acid was purchased from Alfa Aesar; all other reagents were purchased from Sigma-Aldrich or as indicated. 
     Column chromatography was performed on a Teledyne Isco Combiflash Rf, with RediSep Rf Gold normal phase silica columns. 
     Gas chromatography—electron impact mass spectrometry (GC-EIMS) was performed on a Agilent5973-HP6890 GC-MS using a 30 meter db5-ms column 
       1 H NMR and  13 C NMR were obtained on a Bruker AVB 400 MHz spectrometer and a Bruker AV 500 MHz spectrometer at the UC Berkeley College of Chemistry NMR facility, funded in part by NSF grant CHE-0130862. Chemical shifts are reported in ppm relative to residual solvent signal (δ 1 H=3.31 and δ 13 C=49.0 for Methanol-d 4 , δ 1 H=2.05 and δ 13 C=29.84 for Acetone-d 6 ). 
     Succinyl-SNAC 
     A 100 ml round-bottom flask was charged with 1 g of succinic anhydride and dissolved in a minimal volume of dichloromethane (DCM). 1 eq. N-acetylcysteamine (1.07 ml) was added dropwise to the stirring solution. After overnight incubation at ambient temperature with stirring, the mixture was extracted several times with saturated aqueous sodium bicarbonate solution. The pH of this solution was lowered to approximately 6 with dropwise addition of 1 molar hydrochloric acid in order to protonate unreacted N-acetylcysteamine. The mixture was extracted several times with DCM to remove N-acetylcysteamine and then the pH of the aqueous solution was lowered to approximately 3, again with dropwise addition of IM HCl to protonate the title compound. This was extracted several times with ethyl acetate (EtOAc), dried with the addition of sodium sulfate and filtered into a round-bottom flask. The solution was concentrated in vacuo to afford a fluffy white powder (0.663 g, 3.02 mmol, 30.4% yield). 
     Synthesis of 3-Hydroxyadipic Acid Standard 
     Solvent-Free Synthesis of 2-(γ-butyrolactone)acetic acid 
     2-(γ-butyrolactone)acetic acid (systematic name: 2-(5-oxotetrahydrofuran-2-yl)acetic acid), InChI=1S/C6H8O4/c7-5(8)3-4-1-2-6(9) 10-4/h4H, 1-3H2,(H,7,8) 
     2 g of trans-β-hydromuconic acid (13.88 mmol) and 4 g of silica gel (60 Å-200 mesh) were mixed in a 50 mL round-bottom flask with stir bar. The free-flowing mixture was heated to 200° C. in a sand bath while gently stirring. The reaction was monitored by pipetting a few milligrams of the hot mixture into 1 mL of dichloromethane (DCM), of which 40 μL was treated with 10 μL of N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) and analyzed by gas chromatography-mass spectrometry (GC-MS). 3 hours into the reaction the mixture starts to turn yellow. After 24 hours, all starting material had been consumed. The mixture was cooled to room temperature and extracted with 50 mL DCM and filtered. The filtrant was extracted with another 50 mL of DCM. The light yellow filtrate was evaporated under reduced pressure and purified by flash chromatography (70:30 Ethyl acetate:Hexane) to afford the title compound as a viscous slightly yellow liquid that solidified upon standing (474 mg, 3.29 mmol, 24% yield) 
       1 H NMR (500 MHz, MeOD) δ 4.91 (p, J=6.5 Hz, 1H), 2.73 (d, J=6.4 Hz, 2H), 2.66-2.52 (m, 2H), 2.49-2.40 (m, 1H), 2.09-1.92 (m, 1H).  13 C NMR (126 MHz, MeOD) δ 179.68, 173.35, 78.64, 49.00, 40.55, 29.35, 28.31. 
     EIMS (TMS derivative): 201 (7%, (M-Me) + ), 159 (27%), 157 (54%), 117 (11%), 101 (8%), 85 (17%), 76 (7%), 75 (100%), 73 (53%), 59 (9%) 
     Hydrolysis of 2-(7-butyrolactone)acetic acid to Yield 3-hydroxyadipic acid 
     3-hydroxyadipic acid (systematic name: 3-hydroxyhexanedioc acid) InChI=1S/C6H10O5/c7-4(3-6(10) 11)1-2-5(8)9/h4,7H,1-3H2(H,8,9)(H,10,11) 
     30 mg of 2-(7-butyrolactone)acetic (208 μmol) was dissolved in 10.4 mL 0.1 M aqueous potassium hydroxide (5 eq.), distributed among the wells of a 96-well PCR plate and heated to 99° C. for 3 hours in an Applied Biosciences Venti thermocycler with heated lid (105° C.). This was diluted into 100 mM sodium phosphate buffer (pH 6.8) to make standard curves. To acquire NMR data, the solution was consolidated and acidified to pH 3 using 6 M hydrochloric acid. The solution was flash frozen in liquid nitrogen and lyophilized to dryness (˜24 h). The remaining powder was extracted with 2×2 mL acetone and filtered through a pipette filter (KCl has negligible solubility in acetone). At this point 1 μL of the solution was diluted down to a final volume of 40 μL and derivatized with 10 μL BSTFA to yield the GC-MS chromatogram below. The remaining solution was evaporated under reduced pressure at room temperature to yield the title compound as a white powder (26.7 mg, 165 μmol, 79% yield). Re-subjecting the product to GC-MS shows increasing amounts of 2-(γ-butyrolactone) acetic acid over time, suggesting that neat 3-hydroxyadipic acid spontaneously re-lactonizes at room temperature (unlike 3-hydroxyadipic acid solutions in phosphate buffer, which are stable and hence used for standard curves as described above). Hence, the NMR spectrum reported below shows 2-(γ-butyrolactone)acetic acid as an impurity. 
       1 H NMR (500 MHz, MeOD) δ 4.02 (tdd, J=8.6, 4.8, 3.9 Hz, 1H), 2.52-2.33 (m, 4H), 1.90-1.78 (m, 1H), 1.77-1.63 (m, 1H).  13 C NMR (126 MHz, MeOD) δ 179.68, 173.35, 78.64, 49.00, 40.55, 29.35, 28.31. 
     EIMS (tris(TMS) derivative): 363 (32% (M-Me) + ), 247 (26%), 233 (11%), 203 (12%), 149 (14%), 147 (55%), 133 (10%), 129 (24%), 75 (27%), 73 (100%) 
     Synthesis of Acyl-CoAs 
     Synthesis of cyclopentanecarboxyl-CoA (CPMA-CoA) and cyclopentanedicarboxyl-CoA (CPDA-CoA) was previously reported (Hagen et al., 2014) 
     Construct Design and Plasmid Construction 
     AurB, IdmO, and SpnB DNA was generously provided by Ryan Phelan. Codon-optimization and synthesis of AurB was performed by Genscript; codon-optimization and synthesis of IdmO, and SpnB was performed by the Joint Genome Institute (JGI). NanA2 DNA was generously provided by Satoshi Yuzawa; codon-optimization and synthesis was performed by DNA2.0. 
     Amino acid sequences for various modules were aligned using the MUSCLE or Clustal Omega algorithms (Edgar, 2004; Sievers et al., 2014). All DNA pieces were amplified via PCR with either Q5 or Phusion polymerases (New England BioLabs) according to manufacturer&#39;s recommendations. Gel-extracted DNA was assembled via Gibson cloning using Gibson Assembly® master mix (New England BioLabs). In the case of construct A3 (pARH159), Gibson assembly failed and sequence was introduced by oligonucleotides using inverse PCR with pARH137 (A2) as a template. A similar strategy was used to create (A,S)3c constructs (pARH163, 164 respectively) starting from (A,S)2c constructs (pARH147, 149 respectively). A complete list of plasmids follows 
     BorDH2 Monodomain 
     BorDH2 domain boundaries were selected after (Vergnolle et al., 2011) and DNA was codon-optimized and synthesized as a gBlock (Integrated DNA Technologies) and ligated into the pET28a vector (Novagen) to yield an N-terminal hexahistidine tagged construct. 
                     TABLE 1                  Plasmids described in Example 1 herein. Strains may be accessed       and requested through the website for public-registry.jbei.org.                         Strain ID   Alias   Summary               JBx_045172   pARH136   pBbS2k::6xHisMBP-BorA2eloopswap-A1       JBx_045173   pARH137   pBbS2k::6xHisMBP-BorA2eloopswap-A2       JBx_045174   pARH138   pBbS2k::6xHisMBP-BorA2eloopswap-I1       JBx_045175   pARH139   pBbS2k::6xHisMBP-BorA2eloopswap-I2       JBx_045176   pARH140   pBbS2k::6xHisMBP-BorA2eloopswap-N1       JBx_045177   pARH141   pBbS2k::6xHisMBP-BorA2eloopswap-N2       JBx_045178   pARH142   pBbS2k::6xHisMBP-BorA2eloopswap-S1       JBx_045179   pARH143   pBbS2k::6xHisMBP-BorA2eloopswap-S2       JBx_045183   pARH147   pBbS2k::6xHisMBP-BorA2eloopswap-A2c       JBx_045184   pARH148   pBbS2k::6xHisMBP-BorA2eloopswap-I2c       JBx_045202   pARH149   pBbS2k::6xHisMBP-BorA2eloopswap-S2c       JBx_045078   pARH150   pET28a::BorDH2       JBx_045189   pARH159   pBbS2k::6xHisMBP-BorA2eloopswap-A3       JBx_045190   pARH162   pBbS2k::6xHisMBP-BorA2eloopswap-S3       JBx_045191   pARH163   pBbS2k::6xHisMBP-BorA2eloopswap-A3c       JBx_045192   pARH164   pBbS2k::6xHisMBP-BorA2eloopswap-S3c       JBx_045199   pARH176   pBbS2k::6xHisMBP-BorA2eloopswap-S3c-               eryTE                    
Intermediate Analysis of Succinyl-SNAC Extensions:
 
     Samples were analyzed on an AB Sciex (Foster City, Calif.) 4000 Q-Trap mass spectrometer operating in MRM (SRM) mode coupled to an Agilent 1100 system. 1-2 μg of total peptide was injected onto a Sigma (St. Louis, Mich.) Ascentis Peptide Express C-18 column (2.1 mm×50 mm) via an autosampler. A 20.5-minute method was used with a flow-rate of 400 ul/min. The method begins with 95% Buffer A (water, 2% acetonitrile, 0.1% formic acid) and 5% buffer B (water, 98% acetonitrile, 0.1% formic acid) for 1.2 minutes followed by a rapid rise to 25% over 1 minute and then a very slow rise to 36% over 10 minutes. After the slow gradient step, buffer B was rapidly increased to 90%, held, and dropped back down to re-equilibrate the column as above. The peptides eluting from the column were ionized by a Turbo V Ion source (curtain gas flow: 20 l/min, temperature: 400 C, ion spray voltage: 4,800 V, ion source gas flow: 50 l/min, entrance potential: 10 V) operating in positive-ion mode. 
                     TABLE 2                  Mass spectrum parameters for intermediate analysis experiments.                                             Declustering   Collision       ID   Q1   Q3   potential   energy                                         ACP1_ctrl   680.38   846.48 (y8)   125   40       Holo-ACP1   905.76   261.12   50   44       Keto-ADA-ACP1   953.11   403.15   50   44       hydroxy-ADA-ACP1   953.77   405.16   50   44       2,3-ene-ADA-ACP1   947.78   387.16   50   44       ADA-ACP1   948.45   389.17   50   44                    
Intermediate Analysis of Various Acyl-ACP LM  Extensions
 
     Samples were analyzed on an Agilent 6460QQQ mass spectrometer operating in MRM (SRM) mode as previously reported (Dahl et al., 2013). Briefly, 1-2 ug of total peptide was injected on a Sigma Ascentis Peptide Express C-18 column (2.1 mm×50 mm) via an autosampler and separated at 400 ul/min. Liquid chromatography conditions used were as described above. Peptides eluting from the column were ionized using an Agilent Jet Stream source (sheath gas flow: 11 l/min, sheath gas temperature: 350 c, nozzle voltage: 1,000 v, nebulizing pressure: 30 psi, chamber voltage: 4,500 V) operating in positive-ion mode 
     For all experiments, transitions were monitored using a collision cell exit potential of 10 V. 
     
       
         
           
               
            
               
                 (SEQ ID NO: 3) 
               
            
           
           
               
               
            
               
                   
                 ACP1_ctrl peptide: VVESVAFGVPSLR 
               
               
                   
               
            
           
           
               
            
               
                 (SEQ ID NO: 4) 
               
            
           
           
               
               
            
               
                   
                 ACP1 peptide: AAIGPDSSFHAIGFD S LTAVELR 
               
            
           
         
       
     
     (site of phosphopantetheinylation underlined) 
     Methods for SNAC extensions were designed and data collected in Analyst 3.1 and data was quantified in MultiQuant 2.1 (AB Sciex). Methods for acyl-ACP extension were designed in Skyline (MacLean et al., 2010) and data collected in MassHunter (Agilent) 
     Data Analysis 
     Raw data for each transition was normalized by dividing a transition&#39;s peak area by that of a control peptide present in BorMod1, but which does not participate in catalysis and should therefore be invariant across samples (“ACP1_ctrl”) to generate values in “control peptide equivalents.” 
     Adipic Acid Analytical Methods 
     Adipic acid (commercially available) and 3-hydroxy-adipic acid were directly infused into the mass spectrometer operating in negative mode and a scan was conducted to identify product ions during adjustment of relevant acquisition parameters. 
     (3-Hydroxy) Adipic Acid Production 
     Samples were analyzed on an AB Sciex (Foster City, Calif.) 4000 Q-Trap mass spectrometer operating in MRM (SRM) mode coupled to an Agilent 1100 system. 15 ul of each reaction was injected onto a Phenomenex (Torrance, Calif.) Kinetex XB C-18 column (3 mm×100 mm, 1.7 u) via an autosampler. A 24 minute method was used with a flow-rate of 200 ul/min and started with 97.5% Buffer A (water, 0.1% formic acid) and 2.5% Buffer B (acetonitrile, 0.1% formic acid) for 3 minutes followed by a rise to 90% buffer B over 10 minutes where it was held for 2 minutes and then a return to 2.5% Buffer B for 9 minutes to re-equilibrate the column. Analytes eluted from column were ionized using a Turbo V Ion source (curtain gas flow: 20 l/min, temperature: 400 C, ion spray voltage: −4,500 V, ion source gas flow: 60 l/min, entrance potential: −10 V) operating in negative-ion mode. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Mass spectrum parameters for adipic acid and 3-OH-adipic acid 
               
               
                 detection 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Declustering 
                 Collision 
               
               
                   
                 ID 
                 Q1 
                 Q3 
                 potential 
                 energy 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 3-hydroxy 
                 161 
                 99 
                 −45 
                 −18 
               
               
                   
                 Adipic 
                 145 
                 101 
                 −45 
                 −18 
               
               
                   
                   
               
            
           
         
       
     
     FURTHER REFERENCES CITED 
     
         
         Dahl, R. H., Zhang, F., Alonso-Gutierrez, J., Baidoo, E., Batth, T. S., Redding-Johanson, A. M., Petzold, C. J., Mukhopadhyay, A., Lee, T. S., Adams, P. D., et al. (2013). Engineering dynamic pathway regulation using stress-response promoters. Nat. Biotechnol. 31, 1039-1046. 
         Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792-1797. 
         Hagen, A., Poust, S., de Rond, T., Yuzawa, S., Katz, L., Adams, P. D., Petzold, C. J., and Keasling, J. D. (2014). In Vitro Analysis of Carboxyacyl Substrate Tolerance in the Loading and First Extension Modules of Borrelidin Polyketide Synthase. Biochemistry (Mosc.) 53, 5975-5977. 
         Hillson, N. J., Rosengarten, R. D., and Keasling, J. D. (2012). j5 DNA Assembly Design Automation Software. ACS Synth. Biol. 1, 14-21. 
         MacLean, B., Tomazela, D. M., Abbatiello, S. E., Zhang, S., Whiteaker, J. R., Paulovich, A. G., Carr, S. A., and MacCoss, M. J. (2010). Effect of Collision Energy Optimization on the Measurement of Peptides by Selected Reaction Monitoring (SRM) Mass Spectrometry. Anal. Chem. 82, 10116-10124. 
         Sievers, F., Wilm, A., Dineen, D., Gibson, T. J., Karplus, K., Li, W., Lopez, R., McWilliam, H., 
         Remmert, M., Soding, J., et al. (2014). Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol. Syst. Biol. 7, 539-539. 
         Vergnolle, O., Hahn, F., Baerga-Ortiz, A., Leadlay, P. F., and Andexer, J. N. (2011). Stereoselectivity of Isolated Dehydratase Domains of the Borrelidin Polyketide Synthase: Implications for cis Double Bond Formation. ChemBioChem 12, 1011-1014. 
       
    
     While the present invention has been described with reference to the specific embodiments thereof, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the true spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation, material, composition of matter, process, process step or steps, to the objective, spirit and scope of the present invention. All such modifications are intended to be within the scope of the claims appended hereto.