Patent Publication Number: US-4650765-A

Title: Biologically pure culture of Streptoverticillium stramineum sp. nov.

Description:
This is a division of application Ser. No. 510,694, filed July 5, 1983, now U.S. Pat. No. 4,552,867, which is a continuation-in-part of application Ser. No. 332,079, filed Dec. 21, 1981, now abandoned, which is a continuation-in-part of application Ser. No. 237,197, filed Feb. 23, 1981, now abandoned. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of Art 
     This invention relates to two new antibacterial and antitumor agents designated LL-BO1208α and LL-BO1208β and to their production by fermentation of the novel microorganism Streptoverticillium stramineum or mutants thereof. 
     2. Background of the Invention 
     LL-BO1208α and LL-BO1208β are novel antibiotics and anti-tumor agents of the bleomycin/phleomycin family. See, e.g., S. M. Hecht (Ed.), Bleomycin: Chemical, Biochemical and Biological Aspects, Springer-Verlag, New York, 1979. LL-BO1208α and LL-BO1208β are differentiated from all other members of this family by their structure, chemical and physical properties, and effects on specific microorganisms. The most closely related known compound is Tallysomycin A [M. Koniski et al., J. Antibiotics 30: 789 (1977)] which, like LL-BO1208β, contains β-lysine. These two compounds are the only members of the bleomycin/phleomycin family which contain this particular amino acid. However, Tallysomycin A differs significantly from LL-BO1208β in that Tallysomycin A contains spermidine and an additional sugar moiety, and in addition LL-BO1208β contains a reduced bithiazole ring system. 
     BRIEF SUMMARY OF THE INVENTION 
     This invention relates to the novel microorganism Streptoverticillium stramineum and mutants thereof derived spontaneously or by conventional mutagenic or recombinant techniques. 
     This invention also relates to two new antibacterial and anti-tumor agents designated LL-BO1208α and LL-BO1208β, to their production by fermentation, to methods for their recovery and concentration from crude solutions and to processes for their purification. The present invention includes within its scope, these agents in dilute forms, as crude concentrates and in pure crystalline forms. Hereinafter, the term LL-BO1208 refers to a mixture of LL-BO1208α and LL-BO1208β in any proportions. 
     DETAILED DESCRIPTION OF THE INVENTION 
     The compounds of the present invention have the following postulated structure: ##STR1## 
    
    
     The above postulated structures are based on Plasma Desorption Mass Spectra and Fast Atom Bombardment Mass Spectra, which give apparent calculated molecular ions of M +  =m/e 1383 for LL-BO1208α and M +  =m/e 1470 for LL-BO1208β; Infrared (IR) Spectrum, Ultraviolet (UV) Spectrum, Proton Nuclear Magnetic Resonance (&#39;H-NMR) Spectrum, C-Thirteen Carbon Nuclear Magnetic Resonance ( 13  C--NMR) Spectrum, and Electron Impact Mass Spectrum (ElMS) of each compound as shown in the accompanying figures: 
     FIG. I: IR Spectrum of LL-BO1208β in KBr. 
     FIG. II: &#39;H-NMR Spectrum of LL-BO1208β. 79.5 MHz in D 2  O, internal TMS reference equivalent. 
     FIG. III:  13  C-NMR Spectrum of LL-BO1208β. 20 MHz in D 2  O, internal TMS reference equivalent. 
     FIG. IV: UV Spectrum of LL-BO1208β. 10 ug/ml in H 2  O. 
     FIG. V:  13  C-NMR Spectrum of LL-BO1208α. 20 MHz in D 2  O, internal TMS reference equivalent. 
     FIG. VI: &#39;H-NMR Spectrum of LL-BO1208α. 79.5 MHz in D 2  O, internal TMS reference equivalent. 
     FIG. VII: EIMS of LL-BO1208β silylated methyl glycoside. Gulose derivative. 4.6 eV, ion source temperature 250° C. 
     FIG. VIII: EIMS of LL-BO1208β silylated methyl glycoside. Mannose derivative. 46 eV, ion source temperature 250° C. 
     FIG. IX: EIMS of N-trifluoroacetyl-n-butyl ester of threonine. 46 eV, ion source temperature 250° C. 
     FIG. X: EIMS of N-trifluoroacetyl-n-butyl ester of β-alanine. 46 eV, ion source temperature 250° C. 
     FIG. XI: EIMS of N-trifluoroacetyl-n-butyl ester of 4-amino-3-hydoxy-2-methylvaleric acid. 46 eV, ion source temperature 250° C. 
     FIG. XII: EIMS of N-trifluoroacetyl-n-butyl ester of 2,3-diaminopropionic acid. 46 eV, ion source temperature 250° C. 
     FIG. XIII: EIMS of N-trifluoroacetyl-n-butyl ester of β-lysine. 46 eV, ion source temperature 250° C. 
    
    
     The chemical shifts of significant peaks in the  13  C-NMR spectra of LL-BO1208α and LL-BO1208β acetate salts are given in Table I: 
     
                       TABLE I                                                     
______________________________________                                    
Chemical Shifts of Significant Peaks in the .sup.13 C--NRR                
 Spectra.sup.a of LL-BO1208α and LL-BO1208β Acetate Salts      
LL-BO1208α                                                          
               LL-BO1208β                                            
______________________________________                                    
182.3          182.5                                                      
178.8          178.8                                                      
178.6          178.6                                                      
177.5          177.5                                                      
173.3          173.5                                                      
173.1          173.3                                                      
172.7          173.1                                                      
170.4          170.3                                                      
170.2          --                                                         
169.0          169.0                                                      
166.6          166.6                                                      
165.9          165.9                                                      
164.7          164.6                                                      
159.2          159.2                                                      
153.7          153.7                                                      
149.4          149.3                                                      
138.2          138.2                                                      
135.8          135.9                                                      
126.7          127.1                                                      
119.1          119.1                                                      
113.4          113.4                                                      
99.4           99.4                                                       
98.6           98.6                                                       
76.8           76.7                                                       
75.6 (×2)                                                           
               75.6 (×2)                                            
74.8           74.7                                                       
74.1           74.2                                                       
71.5           71.5                                                       
70.4           70.4                                                       
69.6           69.6                                                       
69.2           69.2                                                       
68.5           68.5                                                       
68.3           68.3                                                       
66.0           66.0                                                       
62.2           62.2                                                       
61.5           61.5                                                       
60.9           60.9                                                       
60.2           60.2                                                       
58.3           58.3                                                       
53.8           53.9                                                       
--             49.6                                                       
48.7           48.6 (×2)                                            
48.3           --                                                         
--             45.8                                                       
43.9           43.8                                                       
--             42.0                                                       
41.4           41.4                                                       
--             40.3                                                       
39.8           39.8                                                       
38.7           38.7                                                       
37.9           37.9                                                       
34.2           34.2                                                       
34.0           --                                                         
--             30.3                                                       
24.4           24.5                                                       
--             24.1                                                       
20.1           20.1                                                       
16.0           16.0                                                       
13.1           13.1                                                       
12.0           12.0                                                       
______________________________________                                    
 .sup.a 50.3 MHz spectra obtained in D.sub. 2 O, chemical shifts in parts 
 per million relative to tetramethylsilane.                               
 
    
     The novel agents of the present invention are organic bases and thus are capable of forming acid-addition salts with a variety of organic and inorganic salt-forming reagents. Thus, acid-addition salts are formed by admixture of the antibacterial free base in a neutral solvent with an acid such as, for example, sulfuric, phosphoric, hydrochloric, hydrobromic, sulfamic, citric, maleic, fumeric, tartaric, acetic, benzoic, gluconic, ascorbic, and the like. The acid-addition salts of the agents of this invention are, in general, crystalline solids, soluble in water, methanol and ethanol but insoluble in non-polar organic solvents such as diethyl ether, benzene, toluene and the like. For the purposes of this invention, the antibacterial free bases are equivalent to their pharmaceutically acceptable non-toxic, acid-addition salts. 
     The new antibacterial agents LL-BO1208α and LL-1208β are formed during the cultivation, under controlled conditions, of a new strain of Streptoverticillium stramineum sp. nov. 
     A representative strain of this new antibiotic-producing microorganism was isolated from a grassland soil sample collected in South Dakota and is maintained in the culture collection of the Lederle Laboratories Division, American Cyanamid Company, Pearl River, N.Y. 10965, as culture number LL-BO1208. A viable culture of this new microorganism has been deposited with the Culture Collection Laboratory, Northern Utilization Research and Development Division, U.S. Department of Agriculture, Peoria, Ill. 61604, and has been added to its permanent collection under the accession number NRRL 12292. 
     The following is a general description of the representative strain NRRL 12292 of the microorganism Streptoverticillium stramineum, based on diagnostic characteristics observed. Observations were made of the cultural, physiological and morphological features of the organism in accordance with the methods detailed by Shirling and Gottlieb, Internat. J. of Syst. Bacteriol. 16: 313-340 (1966). Media used in the study were selected from those recommended for the cultivation of actinomycetes by Pridham, et al., Antibiotics Annual pp. 947-953 (1956). The culture was also streaked on media especially employed for the study of Streptoverticillia, Locci, et al., Giorn. Microbiol. 17: 1-60 (1969). Details are recorded in Tables II-IV, wherein the underscored descriptive colors are taken from Jacobson, et al., Color Harmony Manual, 3rd Edition [Container Corp. of America, Chicago, Ill. (1948)]. 
     Micromorphology and Chemotaxonomy 
     Streptoverticillium stramineum NRRL 12292 bears umbels of smooth-surfaced, ovate to rectangular (0.4 μm-0.6 μm×0.75 μm-1.0 μm) spores borne on verticillately-arranged straight sporophores. The cell walls of this strain contain the L-isomer of diaminopimelic acid. 
     Amount of Growth 
     Good on most media; moderate to good on Czapek&#39;s Solution Agar, Benedict&#39;s Agar, Czapek&#39;s Agar plus casamino acids, and Emerson&#39;s Agar. 
     Aerial Mycelium and/or en masse Spore Color 
     Aerial mycelium yellowish; spore masses in yellowish shades, ranging from Light Ivory 2ca to Light Wheat 2ea. Sporulation moderate to heavy depending upon medium. 
     Soluble Pigments 
     Light yellowish to yellowish to tannish when present; absent on several media. 
     Reverse Color 
     In yellowish to light brownish shades. 
     Miscellaneous Physiological Reactions 
     Nitrates not reduced to nitrites; complete liquefaction of gelatin in 14 days; melanoid pigments produced on peptone-iron agar. Carbon source utilization, according to the method of Pridham and Gottlieb, J. Bacteriol. 56: 107-114 (1948) as follows: good utilization of adonitol, d-fructose, glycerol, i-inositol, d-mannitol, d-mannose and dextrose; fair utilization of d-trehalose; and no utilization of l-arabinose, d-galactose, lactose, d-melezitose, d-melibiose, d-raffinose, l-rhamnose, salicin, sucrose and d-xylose. Tolerates 7% sodium chloride but not 10% sodium chloride. 
     
                                           TABLE II                                
__________________________________________________________________________
Cultural Characteristics of Streptoverticillium stramineum NRRL 12292     
Incubation: 14 days                                                       
          AMOUNT OF                                                       
                  AERIAL MYCELIUM                                         
                               Temperature: 28° C.                 
MEDIUM    GROWTH  AND/OR SPORES                                           
                               SOLUBLE PIGMENT                            
                                           REVERSE COLOR                  
                                                      REMARKS             
__________________________________________________________________________
Kuster&#39;s Oatflake                                                         
     Good         Aerial mycelium yel-                                    
                               Light Yellowish                            
                                           Maize                          
Agar              lowish becoming Light    2 hb                           
                  Ivory 2 ca in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Yeast-Malt Agar                                                           
     Good         Aerial mycelium yel-                                    
                               Yellowish   Honey Gold                     
                  lowish becoming Light    2 ic                           
                  Ivory 2 ca in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Bennett&#39;s Agar                                                            
     Good         Aerial mycelium yel-                                    
                               Light Yellowish                            
                                           Maize                          
                  lowish becoming Light    2 hb                           
                  Ivory 2 ca in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Tomato-Paste                                                              
     Good         Aerial mycelium yel-                                    
                               Tannish     Yellow Maple                   
Oatmeal Agar      lowish becoming Light    to                             
                  Wheat 2 ea in sporu-     Light Brown                    
                  lating areas.            3 ng-4 ng                      
                  Sporulation heavy.                                      
Hickey &amp; Tresner&#39;s                                                        
     Good         Aerial mycelium                                         
                               Light Yellowish                            
                                           Yellow Maple                   
Agar              pinkish-yellowish be-    3 ng                           
                  coming Light Wheat                                      
                  2 ea in sporulating                                     
                  areas. Sporulation                                      
                  heavy.                                                  
Asparagine-                                                               
     Good         Aerial mycelium yel-                                    
                               None        Maize                          
Dextrose Agar     lowish becoming Light    2 hb                           
                  Wheat 2 ea in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Czapek&#39;s Solution                                                         
     Modarate     Aerial mycelium yel-                                    
                               None        Cream      Good medium on      
Agar to           lowish becoming Light    11/2 ca    which to ob-        
     Good         Wheat 2 ea in sporu-                serve verti-        
                  lating areas.                       cils                
                  Sporulation moderate.                                   
Pablum Agar                                                               
     Good         Aerial mycelium yel-                                    
                               Tannish     Light Brown                    
                  lowish becoming Light    4 ng                           
                  Wheat 2 ea in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Potato Dextrose                                                           
     Good         Aerial mycelium yel-                                    
                               None        Light Wheat                    
Agar              lowish becoming Light    2 ea                           
                  Wheat 2 ea in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Benedict&#39;s Agar                                                           
     Moderate     Aerial mycelium whit-                                   
                               Light Yellowish                            
                                           Light Maple                    
     to           ish becoming Light       3 ng                           
     Good         Wheat 2 ea in sporu-                                    
                  lating areas.                                           
                  Sporulation moderate.                                   
Rice Agar                                                                 
     Good         Aerial mycelium yel-                                    
                               Light Yellowish                            
                                           Bamboo                         
                  lowish becoming Light                                   
                               to          2 gc                           
                  Wheat 2 ea in sporu-                                    
                               None                                       
                  lating areas.                                           
                  Sporulation heavy.                                      
Weinstein&#39;s                                                               
     Moderate     Aerial mycelium whit-                                   
                               Yellowish   Yellow Maple                   
Agar              ish. No sporulation.     3 ng                           
Inorganic Salts-                                                          
     Good         Aerial mycelium yel-                                    
                               None        Cream      Good medium on      
Starch Agar       lowish becoming Light    11/2 ca    which to ob-        
                  Wheat 2 ea in sporu-                serve vertic-       
                  lating areas.                       ils.                
                  Sporulation heavy.                                      
ADDITIONAL MEDIA FOR OBSERVING SPECIES OF STREPTOVERTICILLIA              
Bennett&#39;s Agar                                                            
          Good    Aerial mycelium yel-                                    
                               Light Yellowish                            
                                           Maize                          
(different        lowish becoming Light    2 hb                           
formulation)      Ivory 2 ca in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Casamino Acids                                                            
          Moderate                                                        
                  Aerial mycelium whit-                                   
                               None        Pearl                          
Czapek&#39;s Agar                                                             
          to      ish to yellowish be-     3 ba                           
          Good    coming Light Ivory                                      
                  2 ca in sporulating                                     
                  areas. Sporulation                                      
                  light to moderate.                                      
Emerson&#39;s Agar                                                            
          Moderate                                                        
                  Aerial mycelium yel-                                    
                               Light Yellowish                            
                                           Honey Gold                     
          to      lowish becoming Light    2 ic                           
          Good    Ivory 2 ca in sporu-                                    
                  lating areas.                                           
                  Sporulation moderate.                                   
Glucose-Asparagine                                                        
          Good    Aerial mycelium yel-                                    
                               None        Light Wheat                    
Agar (ISP #5)     lowish becoming Light    2 ea                           
                  Wheat 2 ea in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Glycerol  Good    Aerial mycelium yel-                                    
                               None        Light Wheat                    
Asparagine Agar   lowish becoming Light    2 ea                           
(ISP #5)          Wheat 2 ea in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
Inorganic Salts-                                                          
          Good    Aerial mycelium yel-                                    
                               None        Light Tan                      
Starch (ISP #4)   lowish becoming Light    3 gc                           
                  Wheat 2 ea in sporu-                                    
                  lating areas.                                           
                  Sporulation heavy.                                      
__________________________________________________________________________
 
    
     
                                           TABLE III                               
__________________________________________________________________________
Miscellaneous Physiological Reaction of Streptoverticillium stramineum    
NRRL 12292                                                                
Temperature: 28° C.                                                
MEDIUM  INCUBATION PERIOD                                                 
                     AMOUNT OF GROWTH                                     
                                  PHYSIOLOGICAL REACTION                  
__________________________________________________________________________
Peptone-Iron                                                              
         48 hours    Good         Moderate amount of melanin pigment      
Agar                              produced.                               
Organic Nitrate                                                           
         7 days      Good         Nitrates not reduced to nitrites        
Broth   14 days      Good         Nitrates not reduced to nitrites        
Purple Milk                                                               
         7 days      Good         Fair to good peptonization              
        14 days      Good         Good peptonization                      
Gelatin  7 days      Good         Partial liquefaction                    
        14 days      Good         Complete liquefaction                   
Yeast Extract                                                             
        10 days                   ≧7% &lt;10%                         
Agar Plus                                                                 
(4,7,10,13%)                                                              
NaCl                                                                      
__________________________________________________________________________
 
    
     
                       TABLE IV                                                    
______________________________________                                    
Carbon Source Utilization Pattern of                                      
Streptoverticillium stramineum NRRL 12292                                 
Incubation: 10 days Temperature: 28° C.                            
CARBON SOURCE                                                             
             UTILIZATION    SPORULATION                                   
______________________________________                                    
Adonitol     Good           Good**                                        
l-Arabinose  None           --                                            
d-Galactose  None           --                                            
d-Fructose   Good           light                                         
Glycerol     Good           very light                                    
i-Inositol   Good           none                                          
Lactose      None           --                                            
d-Mannitol   Good           none                                          
d-Mannose    Good           good**                                        
d-Melibiose  None           --                                            
d-Raffinose  None           --                                            
d-Rhamnose   None           --                                            
Salicin      None           --                                            
Sucrose      None           --                                            
d-Trehalose  Fair           none                                          
d-Xylose     None           --                                            
Dextrose     Good           Very light                                    
d-Melezitose None           --                                            
Negative Control                                                          
             None           --                                            
______________________________________                                    
 **carbon sources especially bringing out color of spores                 
 
    
     It is to be understood that the term Streptoverticillium stramineum is not limited to strain Streptoverticillium stramineum NRRL 12292 or to strains fully answering the above growth and microscopic characteristics, which are given for illustrative purposes only. Streptoverticillium stramineum described herein includes all strains of Streptoverticillium stramineum which produce the antibiotics LL-BO1208α or LL-BO1208β or combinations thereof and which cannot be definitely differentiated from Streptoverticillium stramineum NRRL 12292 and its subcultures, including mutants and variants thereof. The term &#34;mutants&#34; includes the natural (spontaneous) mutants of this organism as well as induced mutants produced from this organism by various mutagenic means known to those skilled in the art, such as exposure to x-ray radiation, ultraviolet radiation, nitrogen mustard, actinophages, nitrosamines, and the like. It is also desired and intended to include inter- and intraspecific genetic recombinants produced by genetic techniques known to those skilled in the art, such as for example conjugation, transduction, and genetic engineering techniques. 
     Fermentation Process 
     Cultivation of Streptoverticillium stramineum may be carried out in a wide variety of liquid media. Media which are useful for the production of these novel antibacterial agents include an assimilable source of carbon such as starch, sugar, molasses, glycerol, etc.; an assimilable source of nitrogen such as protein, protein hydrolysate, polypeptides, amino acids, corn steep liquor, etc.; and inorganic anions and cations, such as potassium, sodium, ammonium, calcium, sulfate, carbonate, phosphate, chloride, etc. Trace elements such as boron, molybdenum, copper, etc. are supplied as impurities of other constituents of the media. Aeration in tanks, bottles and flasks is provided by forcing sterile air through or onto the surface of the fermenting medium. Further agitation in tanks is provided by a mechanical impeller. An anti-foaming agent such as lard oil or silicone defoamer may be added as needed. 
     Inoculum Preparation 
     Shaker flask inoculum of Streptoverticillium stramineum is prepared by inoculating 100 ml. portions of sterile liquid medium in 500 ml. flasks with scrapings or washing of spores from an agar slant of the culture. The following is an example of a suitable seed medium. 
     
         ______________________________________                                    
Corn starch           24     g.                                           
Dextrose              5      g.                                           
Yeast extract         5      g.                                           
tryptone.sup.1        5      g.                                           
Beef extract          3      g.                                           
Calcium carbonate     4      g.                                           
Water to              1000   ml.                                          
______________________________________                                    
 [.sup.1 A peptone, registered trademark of Difco Laboratories, Detroit,  
 Michigan                                                                 
 
    
     These flasks are incubated at 23°-35° C., preferably at 28° C., with agitation for 22 to 26 hours, and are then used to inoculate 12 liters of the same sterile medium in a bottle which, after incubation with aeration at 28° C. for 24 hours, is used to inoculate 300 liters of the same medium in a seed tank. This inoculum is incubated at 28° C. for 24 hours with impeller agitation at 175 r.p.m. and sterile air flow of 150 liters per minute and then used to inoculate tank fermentors. 
     Tank Fermentation 
     The following is a suitable medium for the production of LL-BO1208α and -β in fermentation tanks. 
     
         ______________________________________                                    
Lard Oil               30     kg.                                         
Pharmamedia ®.sup.2                                                   
                       15     kg.                                         
Corn steep liquor      15     kg.                                         
Ammonium sulfate       4.5    kg.                                         
Zinc sulfate heptahydrate                                                 
                       45     kg.                                         
Calcium carbonate      6      kg.                                         
Water to               1500   liters                                      
______________________________________                                    
 [.sup.2 defatted cotton seed meal, registered trademark of Trader&#39;s      
 Protein Div. of Trader&#39;s Oil Mill, Ft. Worth, Texas                      
 
    
     Each tank is inoculated with 3 to 10% of inoculum prepared as described above. Aeration is supplied at 750 liters of air per minute and the mixture is agitated by an impeller driven at 100 r.p.m. The temperature is maintained at 24°-35° C. The fermentation is continued for 80-120 hours, at which time the mash is harvested. 
     Isolation of LL-BO1208 
     During fermentation under the controlled conditions described above, Streptoverticilloium stramineum sp. nov. produces antibiotic agent LL-BO1208 (defined as a mixture of LL-BO1208α AND LL-BO1208β in any proportions). This LL-BO1208 may be isolated as follows (all procedures described below are carried out at 5°-30° C., preferably at 20°-25° C., unless otherwise specified): 
     A fermentation is carried out as described above and the harvest mash is filtered to remove cellular debris. The antibiotic LL-BO1208 is then collected and purified by conventional techniques well-known to those skilled in the art, such as for example adsorption chromatography, column chromatography, cation exchange chromatography, thin-layer chromatography, or selective precipitation. 
     The preferred process for collecting and purifying LL-BO1208 comprises adjusting the pH of the harvest mash filtrate to a pH of about 7.0-7.5 and adsorbing said filtrate batchwise on a cation-exchange material. Suitable cation-exchange materials are those having a weakly acidic function and an open structure such as for example CM-Sephadex®C-25(Na + ), carboxymethyl cellulose, or Amberlite IRC-50®. CM-Sephadex®25(Na+) is preferred. The adsorbed LL-BO1208 is eluted with a high concentration of a high-ionic strength salt such as for instance about 7-14% sodium chloride (preferably about 8%) or about 7-14% potassium chloride (preferably about 8%). 8% sodium chloride is preferred. The salt is then removed by conventional means for desalting water-soluble materials, such as for example adsorption chromatography, high-pressure liquid chromatography, or mixed-bed resin chromatography. The preferred method of desalting is adsorption chromatography. The antibiotic salt is adsorbed on a suitable adsorbant column such as for example Amberlite®XAD-2, granular carbon, Amberlite®XAD-4, or Amberlite®XAD-7. The preferred adsorbant is Amberlite®XAD-2.  The interstitial salt is washed out with water and the LL-BO1208 is then eluted with a 10:90 to 30:70 (v/v) solution of demineralized water:neutral polar water-miscible solvent, such as for instance ethanol, methanol, acetone, etc. Preferably, a 30:70 water:methanol (v/v) solution is used. 
     The resulting salt-free antibiotic LL-BO1208 eluate may be desolventized in vacuo and freeze-dried for storage. 
     If desired, the LL-BO1208 may be separated into its constituents LL-BO1208α and LL-BO1208β by conventional means well-known to those skilled in the art, such as for example column chromatography, adsorption chromatography, cation-exchange chromatography, thin-layer chromatography, or selective precipitation. The preferred methods of separating LL-BO1208α and LL-BO1208β from the mixture LL-BO1208 obtained as above are as follows: 
     Isolation of LL-BO1208α and LL-BO1208β 
     Salt-free eluent of LL-BO1208 as described above, or a freeze-dried portion of said eluent dissolved in water, is adsorbed on a cation-exchange material. Examples of suitable cation-exchange materials are those having a weakly acidic function and an open structure, such as for example CM-Sephadex®25(Na + ) (preferred), carboxymethyl cellulose, Amberlite IRC-50(Na + )®, or Amberlite IRC-72®. The antibiotic LL-BO1208 is then separated into its components LL-BO1208α and LL-BO1208β by chromatographic development with an appropriate high-ionic-strength linear salt gradient. Examples of appropriate gradients are, for example, 0.1M to 2M ammonium acetate (0.1M to 1M preferred), 0.1M to 1M ammonium formate, or 1% to 7% sodium chloride. Of these, 0.1M to 1M ammonium acetate is most preferred. The eluate fractions are monitored for the presence of antibiotic by conventional techniques well-known to those skilled in the art, such as for example by determining light absorption at 290 nm or by assaying for biological activity. The LL-BO1208α  is eluted first at the lower salt concentration; followed by the LL-BO1208β at the higher salt concentration. The active fractions for each antibiotic are pooled. 
     In the most preferred process, the components LL-BO1208α and LL-BO1208β are isolated by chromatographic development in separate gradients. In this process, LL-BO1208α is separated from LL-BO1208β by development on CM-Sephadex®25(Na + ) with a 0.1M-0.4M ammonium acetate gradient and only LL-BO1208α is collected. The LL-BO1208β is isolated by separation from LL-BO1208 on CM-Sephadex®25(Na + ) with a 0.1-1.0M ammonium acetate gradient, and only LL-BO1208β is collected. 
     Purification of LL-BO1208α and LL-BO1208β 
     Once separated, the antibiotics LL-BO1208 and LL-BO1208 may be purified by conventional means, such as for example adsorption on and elution from Amberlite XAD-2® as described above. 
     The preferred process for purification of either antibiotic LL-BO1208α or LL-BO1208β is as follows: the appropriate fractions from the salt gradient eluate are pooled and a sufficient amount of complexing agent is added to completely complex the antibiotic. Examples of appropriate complexing agents are those having the properties of forming insoluble sulfides and the ability to complex with the antibiotic at stable pH ranges, such as for example cupric acetate, cobalt acetate, or zinc acetate. The cupric ion is the preferred agent. The amount of complexing agent needed can be readily determined by those skilled in the art. The complexed antibiotic is desalted as described above, and the desalted antibiotic-metal complex is concentrated in vacuo and freeze-dried, giving a blue solid. This solid is dissolved in a neutral polar water-miscible solvent such as for example ethanol, methanol, or acetone. To this is added an equal volume of the same solvent saturated with a compound suitable for decomplexing the antibiotic, such as for example hydrogen sulfide. A small amount of clarifying agent, e.g. activated charcoal, diatomaceous earth, clays or silica gel, is added and the mixture is stirred for about 5 to 30 minutes, preferably for about 15 minutes, and then filtered. The filtrate is diluted with 1/4 to 2 volumes (preferably about 1/3 volume) of water and concentrated in vacuo at 25°-50° C. (preferably at about 35° C.) to an aqueous phase, which is then filtered through an ultra-fine filter, e.g. a Nalgene® plain membrane filter with pores of 0.20 microns, Pall Ultipore® membrane filters, or Pyrex® brand funnels with ultrafine or fine fritted discs (preferred), and freeze-dried. 
     These antibacterial agents were compared in vitro using a variety of gram positive and gram negative bacteria by the standard agar dilution procedure. The results are reported as minimal inhibitory concentrations in mcg./ml. in Table V. The antibacterial agents were not active against strains of Pseudomonas, Enterococcus, and anaerobic bacteria at concentrations of 128 mcg./ml. or less. 
     
                       TABLE V                                                     
______________________________________                                    
                   MINIMAL                                                
                   INHIBITORY                                             
                   CONCENTRATION                                          
                   (mcg./ml.)                                             
                     LL-       LL-                                        
ORGANISM             BO1208α                                        
                               BO1208β                               
______________________________________                                    
Salmonella sp. Arizona QHC 77-3                                           
                     2         0.5                                        
Salmonella sp. Indiana                                                    
                     1                                                    
Salmonella infantis  2                                                    
Salmonella sp. Heidelberg                                                 
                     1                                                    
Salmonella typhimurium K                                                  
                     1                                                    
Acinetobacter sp. Stamford 7743                                           
                     32        2                                          
Acinetobacter sp. K 77-1                                                  
                     64        32                                         
Acinetobacter sp. K 77-5                                                  
                     16                                                   
Acinetobacter sp. UR 75-20                                                
                     32                                                   
Acinetobacter sp. MAYO 75-10                                              
                     16                                                   
Staphylococcus aureus SSC 18                                              
                     4                                                    
Staphylococcus aureus SSC 80                                              
                     4                                                    
Staphylococcus aureus SSC 22                                              
                     4                                                    
Staphylococcus aureus SSC 73                                              
                     8                                                    
Staphylococcus aureus FU 19-4                                             
                     8                                                    
Staphylococcus aureus FU 19-2                                             
                     8         2                                          
Staphylococcus aureus SSC 79-18                                           
                     8         1                                          
Klebsiella sp. Stamford 6404                                              
                     8         1                                          
Klebsiella sp. SSC 78-1                                                   
                     8         1                                          
Enterobacter aerogenes Stamford 6315                                      
                     16        2                                          
Enterobacter aerogenes K 79-16                                            
                     8         4                                          
Enterobacter aerogenes K 79-17                                            
                     4                                                    
Enterobacter aerogenes K 79-18                                            
                     4                                                    
Enterobacter cloacae Schaefler 60                                         
                     32                                                   
Enterobacter cloacae K 79-16                                              
                     8         4                                          
Enterobacter agglomeraus SM 77-1                                          
                     8                                                    
Serratia sp. TUL 78-15                                                    
                     128       8                                          
Serratia sp. QHC 77-2                                                     
                     64        8                                          
Serratia sp. TUL 78-17                                                    
                     64                                                   
Serratia sp. UCLA 79-1                                                    
                     64                                                   
Serratia sp. UCLA 79-3                                                    
                     64                                                   
Proteus morganii Schaefler 71                                             
                     16                                                   
Proteus morganii K 79-25                                                  
                     64        8                                          
Proteus rettgeri Schaefler 72                                             
                     2                                                    
Proteus rettgeri K 77-5                                                   
                     256                                                  
Proteus rettgeri N 76-1        32                                         
Proteus vulgaris SM 77-1                                                  
                     32                                                   
Proteus vulgaris K 77-3                                                   
                     32                                                   
Escherichia coli Stamford 7037                                            
                     0.5       0.25                                       
Escherichia coli 311 2         0.25                                       
Escherichia coli Schaefler 59                                             
                     0.5                                                  
Escherichia coli Stamford 6341                                            
                     1                                                    
Escherichia coli K 79-1                                                   
                     2                                                    
Escherichia coli K 79-4                                                   
                     0.5                                                  
Escherichia coli SM 77-16                                                 
                     0.5                                                  
Klebsiella pneumoniae Schaefler 58                                        
                     4                                                    
Klebsiella pneumoniae Schaefler 67                                        
                     4                                                    
Klebsiella pneumoniae Stamford 6404                                       
                     4                                                    
Klebsiella pneumoniae SSC 78-1                                            
                     4                                                    
Klebsiella pneumoniae SSC 78-10                                           
                     8                                                    
Klebsiella pneumoniae K 79-8                                              
                     2                                                    
______________________________________                                    
 
    
     The antibacterial agents LL-BO1208α and LL-BO-1208β are also active in vivo against a variety of organisms. These new antibacterials are thereby potentially useful as therapeutic agents in treating bacterial infections in mammals. 
     The usefulness of these new antibacterial agents is demonstrated by their ability to control systemic lethal infections in mice. These new substances show high in vivo antibacterial activity in mice against Klebsiella pneumoniae AD and Escherichia coli 311 when administered subcutaneously in a single dose to groups of Charles River CD-1 female mice weighing 20±2 g. each, infected intraperitoneally with a lethal dose of these bacteria in a trypticase soy broth (TSB) dilution of a 5 hour TSB culture. Table VI gives the in vivo activity of LL-BO1208α and -β against these bacteria. 
     
                                           TABLE VI                                
__________________________________________________________________________
               ALIVE/TOTAL MICE TESTED 7 DAYS AFTER INFECTION             
                KLEBSIELLA                                                
SINGLE SUBCUTANEOUS                                                       
               PNEUMONIAE AD   ESCHERICHIA COLI 311                       
DOSE (mg./kg.) LL BO1208α                                           
                       LL BO1208β                                    
                               LL BO1209α                           
                                       LL BO1208β                    
__________________________________________________________________________
16             5/5             5/5                                        
8              4/5             0/5                                        
4              0/5     5/5     0/5     5/5                                
2                      4/5             3/5                                
1                      2/5             0/5                                
0.5                    3/5                                                
0.25                   0/5                                                
__________________________________________________________________________
 
    
     In addition to their antibacterial activity, these agents are effective in suppressing the growth of tumors in warm-blooded animals as substantiated by the results of the following tests. 
     Activity Against B16 Melanoma in Mice 
     Male mice (BDF 1 ) are inoculated subcutaneously with 0.5 ml. of a 10% homogenate of B16 melanoma on day zero. The test compounds are administered intraperitoneally, at the indicated dosages, on days one through nine following tumor inoculation. Tumor size is measured on day 13 with a positive response being indicated by a 58% or greater inhibition of tumor size as compared to controls. The results of this test appear in Table VII. 
     
                                           TABLE VII                               
__________________________________________________________________________
Effect on Tumor Growth in Mice with Subcutaneous B16 Melanoma             
                 NO. SURVIVORS                                            
                           AVG. TUMOR                                     
                                   % TUMOR                                
COMPOUND                                                                  
        DOSE (mg./kg.)                                                    
                 NO. TREATED                                              
                           WEIGHT (g.)                                    
                                   INHIBITION                             
__________________________________________________________________________
Control (saline) 10/10     605                                            
LL-BO1208α                                                          
        16        8/10     212     65.0                                   
        8        10/10     217     64.1                                   
        4        10/10     240     60.3                                   
LL-BO1208β                                                           
        8         9/10     127     79.0                                   
        4         8/10     200     66.9                                   
__________________________________________________________________________
 
    
     In addition to inhibiting tumor growth, these agents also increase the life span of warm-blooded animals bearing B16 melanoma. Table VIII gives the results of a test conducted in accordance with the above described procedure, with an increase in life span of 25% or more being considered significant. 
     
                                           TABLE VIII                              
__________________________________________________________________________
Effect on Survival Time of Mice Bearing                                   
Subcutaneous B16 Melanoma                                                 
                 MEDIAN SURVIVAL                                          
                             % INCREASE IN                                
COMPOUND                                                                  
        DOSE (mg./kg.)                                                    
                 (DAYS)      LIFE SPAN                                    
__________________________________________________________________________
Control (saline) 29.0                                                     
LL-BO1208α                                                          
        16       38.0        31                                           
        8        41.5        43                                           
        4        46.5        60                                           
LL-BO1208β                                                           
        4        42.0        45                                           
        2        43.0        48                                           
        1        38.5        33                                           
__________________________________________________________________________
 
    
     Activity Against Lewis Lung Carcinoma in Mice 
     Mice (BDF 1 ) receive 0.5 ml. of a 10% tumor homogenate subcutaneously. The test compounds are administered intraperitoneally at the indicated doses on days one through nine following tumor inoculation. Tumor size is measured on day 13 with a positive response being indicated by a 58% or greater inhibition of tumor size as compared to controls. The results of this test appear in Table IX. 
     
                                           TABLE IX                                
__________________________________________________________________________
Effect on Tumor Growth in Mice with Subcutaneous Lewis Lung Carcinoma     
                 NO. SURVIVORS                                            
                           AVG. TUMOR                                     
                                   % TUMOR                                
COMPOUND                                                                  
        DOSE (mg./kg.)                                                    
                 NO. TREATED                                              
                           WEIGHT (g.)                                    
                                   INHIBITION                             
__________________________________________________________________________
Control (Saline) 9/10      3836                                           
LL-BO1208β                                                           
        12       2/10      0       100                                    
        8        9/10      76      98                                     
        4        9/10      389     90                                     
        2        10/10     928     76                                     
        1        10/10     1103    71                                     
__________________________________________________________________________
 
    
     In addition to inhibiting tumor growth, these agents also increase the life span of warm-blooded animals bearing Lewis lung carcinoma. Table X gives the results of a test conducted in accordance with the above described procedure, with an increase in life span of 25% or more being considered significant. 
     
                                           TABLE X                                 
__________________________________________________________________________
Effect on Survival Time of Mice Bearing Lewis Lung Carcinoma              
                 MEDIAN SURVIVAL                                          
                             % INCREASE IN                                
COMPOUND                                                                  
        DOSE (mg./kg.)                                                    
                 (DAYS)      LIFE SPAN                                    
__________________________________________________________________________
Control (saline) 26                                                       
LL-BO1208β                                                           
        8        32          25                                           
        4        33          27                                           
        2        33          27                                           
__________________________________________________________________________
 
    
     The invention will be described in greater detail in conjunction with the following specific examples. 
     EXAMPLE 1 
     Inoculum Preparation 
     An inoculum medium was prepared according to the following formulation: 
     
         ______________________________________                                    
Corn starch           24     g.                                           
Dextrose              5      g.                                           
Yeast extract         5      g.                                           
tryptoneTM.           5      g.                                           
Beef extract          3      g.                                           
Calcium carbonate     4      g.                                           
Water to              1000   ml.                                          
______________________________________                                    
 
    
     Washed or scraped spores from an agar slant of Streptoverticillium stramineum NRRL 12292 were used to inoculate a 500 ml. flask containing 100 ml. of the above sterile medium. The flask was placed on a rotary shaker and agitated vigorously at 28° C. for 24 hours. The resulting flask inoculum was transferred to a 12-liter bottle containing the same sterile medium and incubated at 28° C. for 24 hours with aeration. The resulting bottle medium was used to inoculate a tank containing 300 liters of the same sterile medium which was then incubated at 28° C. for 24 hours with agitation by an impeller driven at 175 r.p.m. and aeration by a sterile air flow of 150 liters per minute, producing the tank inoculum. 
     EXAMPLE 2 
     Fermentation 
     A fermentation medium was prepared according to the following formula: 
     
         ______________________________________                                    
Lard oil               30     kg.                                         
Pharmamedia ®      15     kg.                                         
Corn steep liquor      15     kg.                                         
Ammonium sulfate       4.5    kg.                                         
Zinc sulfate heptahydrate                                                 
                       45     g.                                          
Calcium carbonate      6      kg.                                         
Water to               1500   liters                                      
______________________________________                                    
 
    
     This fermentation medium was sterilized at 120° C. for 60 minutes and then inoculated with 150 liters of the tank inoculum prepared in Example 1. The fermentation was carried out at 28° C. using Hodag®FD82 [a silicone antifoam, registered trademark of Hodag Chemical Corp., Skokie, Ill.] as a defoaming agent. Aeration was supplied at 750 liters of sterile air per minute. The mash was agitated by impellers driven at 100 r.p.m. At the end of approximately 87 hours of fermentation time, the mash was harvested. 
     EXAMPLE 3 
     Preliminary Isolation of LL-BO1208α and β 
     A fermentation was carried out as described in Example 2. The 1500 liters of harvest mash was combined with 3% (w/v) Celite®512 [a diatomaceous earth filter aid, registered trademark of Johns-Manville Sales Co., Englewood Cliff, N.J.] and filtered through a plate and frame press. The press cake was washed with 150 liters of water and the water wash and filtrate were combined. The pH was 7.1 and no adjustment to the desired 7.0-7.5 range was required. The antibiotics were adsorbed batchwise on CM-Sephadex®C-25 (Na + ) [a carboxylated dextran ion exchange material, registerred trademark of Pharmacia Fine Chemicals Div. of Pharmacia, Inc., Piscataway, N.J.]. The resin was stirred for 11/2 hours, then allowed to settle for 2 hours and the supernatant was syphoned off and discarded. The resin was washed batchwise 3-5 times with water and then slurried in a volume of 8% sodium chloride solution equal to 1/2 the settled resin volume. the mixture was stirred 10 minutes, then transferred to a large (18×22 inch) stainless steel column. The &#34;batch salt&#34; effluent was collected by gravity flow from the column. When the liquid head above the resin in the column reached the top of the resin bed, fresh 8% sodium chloride solution was fed into the column. Twenty liter fractions were collected. The active fractions were determined by bioassays. These active fractions from the sodium chloride elution were percolated through a 5 liter bed volume of Amberlite®XAD-2 [a synthetic polymeric non-ionic adsorbant, registered trademark of Rohm and Haas, Philadelphia, Pa.] resin in a 10.2×152.4 cm. glass column. This resin was washed with demineralized water and fractions were collected in one to four liters which were pooled. The resin was then eluted with a 70% methanol:30% demineralized water mixture, and fractions were collected in four liter units which were pooled. Each of these pooled fractions was separately concentrated and freeze-dried. 
     EXAMPLE 4 
     Isolation of LL-BO1208β 
     A 9.0 g. portion of the freeze-dried material from the pooled water elution fractions of the charged Amberlite®XAD-2 resin column of Example 3 was chromatographed on a CM-Sephadex®C-25 (Na+) column. The solid was dissolved in water and allowed to seep into the column. The charge was then washed into the column with water and then developed with a gradient between 0.1M and 1.0M ammonium acetate. A series of column fractions of 75 ml. were collected and monitored both by U.V. absorbance of a 1:20 dilution at 202 nm and by bioautography. Fractions 74-83 were combined, 200 mg. of copper acetate were added and the combined fractions were desalted using 300 ml. of Amberlite®XAD-2 resin, giving 1548 mg. of a blue solid. A 600 mg. portion of this solid was dissolved in 75 ml. of methanol. A 75 ml. portion of methanol saturated with hydrogen sulfide was added, together with a small portion of activated charcoal and the mixture was stirred for 15 minutes. This suspension was filtered. The filtrate was diluted with 50 ml. of water and concentrated in vacuo to an aqueous phase, which was then filtered through an ultra-fine filter. This filtrate was freeze-dried, giving 398 mg. of LL-BO1208β, having the following characteristics: 
     Microanalysis: C, 44.82; H, 6.50; N, 15.41; S, 3.94. 
     Specific Rotation [alpha] D   25  =+17±2. 
     LL-BO1208β has an Infrared Absorption Spectrum as shown in FIG. I; a Proton Magnetic Resonance Spectrum as shown in FIG. II; a C 13  -Nuclear Magnetic Resonance Spectrum as shown in FIG. III; and an Ultraviolet Absorption Spectrum as shown in FIG. IV, all of the attached drawings. 
     EXAMPLE 5 
     Isolation of LL-BOL208α 
     A series of freze-dried preparations from the pooled water and pooled methanol elution fractions from charged Amberlite®XAD-2 resin columns, prepared as described in Example 3, and from fermentations conducted essentially as described in Example 2, totalling 5.552 g. were combined and dissolved in 150 ml. of water. The solution was allowed to percolate into a 4.5×70 cm. column of CM-Sephadex®-25(Na+). The column was then developed with a gradient between 0.1M and 0.4M ammonium acetate. Fractions of about 40 ml. were collected and monitored for the presence of LL-BO1208α by taking optical density readings at 202 nm., after a 21 fold dilution with water. Fractions 62-71 were combined and desalted by adsorption and elution from an Amberlite®XAD-2 column as described in Example 4. The eluates were freeze-dried giving 387 mg. of solid. This solid was dissolved in 100 ml. of methanol saturated with hydrogen sulfide. The solution was stirred with a small portion of activated charcoal for 20 minutes, filtered, desolventized and freeze-dried, giving 303 mg. of LL-BO1208α having the following characteristics: Microanalysis: C, 43.18; H, 5.74; N, 15.47; S, 3.39. 
     Specific Rotation [alpha] D   25  =+19. 
     LL-BO1208α has a C 13  -Nuclear Magnetic Resonance Spectrum as shown in FIG. V and Proton Magnetic Resonance Spectrum as shown in FIG. VI, both of the attached drawings. 
     EXAMPLE 6 
     Gas Chromatographic/Mass Spectrum of LL-BO1208α and LL-BO1208β Silylated Methyl Glycoside 
     A one mg. portion of LL-BO1208β in one ml. of 1.5M methanolic hydrogen chloride was heated in a sealed glass ampoule for 4 hours at 110° C. The methanoylsate was transferred to a one ml. tapered vial fitted with a Teflon® (Dupont Co., Wilmington, Del.) coated rubber seal and the acid methanol was removed in a stream of nitrogen with some warming and then freeze-dried for 16 hours. A 100μ portion of Tri-Sil &#34;Z&#34; [trimethylsilylimidazole, trimethylsilylating agent, registered trademark of Pierce Chemical Co., Rockford, Ill.] was added to the freeze-dried residue and this mixture is allowed to stand at room temperature for 30 minutes prior to injection on a gas chromatographic unit. The silylated methyl glycoside of LL-BO1208α was prepared in a similar manner. The gas chromatographic/mass spectrum analysis for each was performed as follows: 
     Column: 3% OV-17 [an essentially 50:50 methyl:phenyl silicone, registered trademark of Ohio Valley Chemicals, Marietta, Ohio] on 100/120 GCQ (GAS CROM Q® [a silanized activated diatomaceous earth solid support, registered trademark of Applied Science Laboratories, Inc., State College, PA.]) (6 feet×2 mm.). 
     Program: 140°-280° C., 6° C. per minute. 
     Helium (carrier gas): 30 ml. per minute; hydrogen: 30 ml. per minute; air: 30 ml. per minute. 
     Detector temperature: 275° C. 
     Injector temperature: 218° C. 
     Chart speed: 1/4 inch per minute. 
     Instrument: Varian CH-7 Mass Spectrometer. 
     Samples of bleomycin sulfate, zorbamycin, mannose and glucose were treated in exactly the same manner. 
     By mass spectral data the silyated methyl glycoside of LL-BO1208α and LL-BO1208β contain a derivative identical to the L-gulose derivative obtained from the silyated methyl glycoside of bleomycin: ##STR2## 
     Retention time 4.6 minutes; G.C. intensity 1.2 volts; calculated molecular weight 482; G.C./M.S. as shown in FIG. VII of the attached drawings for LL-BO1208β. 
     By the same procedure, LL-BO1208α and LL-BO1208β are shown to contain a mannose derivative identical to the silylated methyl glycoside of D-mannose: ##STR3## 
     Retention time 5.4 minutes; G.C. intensity 3.3 volts; calculated molecular weight 482; G.C./M.S. as shown in FIG. VIII of the attached drawings for LL-BO1208β. 
     EXAMPLE 7 
     Gas Chromatographic/Mass Spectrum of N-trifluoroacetyl-n-butyl Esters of Amino Acids Obtained by Acid Hydrolysis of LL-BO1208α and LL-B1208β 
     A 3 mg. portion of LL-BO1208β in 3 ml. of 6N hydrochloric acid was heated for 24 hours at 110° C. The acid residue was washed several times with water on a rotary evaporator to remove excess hydrochloric acid and then placed under high vacuum for 16 hours. 
     The process for preparing the N-trifluoroacetyl-n-butyl ester was essentially that of Pandey, et al., J.A.C.S. 99(26): 8469-8483 (Dec. 21, 1977). A 0.5 ml. portion of acidic butanol (2.4 ml. of acetyl chloride in 10 ml. of n-butanol) was added to the acid hydrolysis product and the mixture was heated for 30 minutes at 100±2° C. in a sealed glass ampoule. This partial derivative was placed under high vacuum for 16 hours. A one ml. portion of methylenechloride and 0.5 ml. of trifluoroacetic anhydride were added to the freeze-dried partial derivative and the mixture was heated for 5 minutes at 150±2° C. The derivative was cooled to 0° C. under nitrogen and any residual solvent was removed. Fresh methylene chloride was added to give a final concentration of 1%. The N-trifluoroacetyl-n-butyl ester of LL-BO1208α was prepared in the same manner. 
     The gas chromatographic/mass spectrum analysis of LL-BO1208α and LL-BO1208β was performed as follows: 
     Column: 3% OV-17® in GCQ (100-120 mesh) (6 feet×2 mm.) 
     Program: 80°-280° C., 8° C. per minute. 
     Helium flow: 33 ml. per minute. 
     Detector temperature: 277° C. 
     Injector temperature: 215° C. 
     Chart speed: 1/4 inch per minute. 
     Instrument: Varian CH-7 Mass Spectrometer. 
     Samples of β-alanine, DL-threonine, streptothricin and bleomycin sulfate were treated in exactly the same manner. 
     By mass spectral studies the amino acids obtained from acid hydrolysis of LL-BO1208α and -β and detected as their N-trifluoroacetyl-n-butyl esters include the following: 
     
         ______________________________________                                    
 ##STR4##             Retention time 4.4 minutes; G.C. intensity 8.0      
                      volts; calculated mole- cular weight 367; G.C./M.S. 
                      as shown in FIG. IX of the at- tached drawings for  
                      LL-BO1208β.                                    
 ##STR5##             Retention time 6.3 minutes; G.C. intensity 6.2      
                      volts; calculated mole- cular weight 241; G.C./M.S. 
                      as shown in FIG. X of the at- tached drawings for   
                      LL-BO1208β.                                    
 ##STR6##             Retention time 7.6 minutes; G.C. intensity 3.9      
                      volts; calculated mole- cular weight 395; G.C./M.S. 
                      as shown in FIG. XI of the at- tached drawings for  
                      LL-BO1208β.                                    
 ##STR7##             Retention time 9.4 minutes; G.C. intensity one      
                      volts; calculated mole- cular weight 352; G.C./M.S. 
                      as shown in FIG. XII of the attached drawings for   
                      LL-BO1208β.                                    
β-amino-β-(4-amino-6-car-                                       
                      Retention time                                      
boxy-5-methylpyrimidin-2-                                                 
                      10 minutes;                                         
yl)propionic acid deriva-                                                 
                      GC intensity 6.6 volts;                             
tive:                 calaulated molecular                                
                      weight 448.                                         
 ##STR8##                                                                 
______________________________________                                    
 
    
     In addition, LL-BO1208β contains a β-lysine derivative: 
     
         __________________________________________________________________________
 ##STR9##                     Retention time 15.6 minutes; G. C.          
                              intensity 8.1 volts; calculated molecular   
                              weight 394; G.C./M.S. as shown in FIG. XIII 
                              of the attached drawings.                   
__________________________________________________________________________