Patent Publication Number: US-2018043005-A1

Title: Immunogenic composition comprising elements of c. difficile cdtb and/or cdta proteins

Description:
CROSS-REFERENCE 
     This application is a Continuation of copending U.S. patent application Ser. No. 14/653,989 filed Jun. 19, 2015, which is a 371 National Stage of PCT International Application No. PCT/EP2013/077762 filed Dec. 20, 2013, which claims the benefit under 35 U.S.C. §119(a) to Patent Application No. UK 1223342.5 filed Dec. 23, 2012, all of which are hereby expressly incorporated by reference into the present application 
    
    
     BACKGROUND 
       C. difficile  is the most important cause of nosocomial intestinal infections and is the major cause of pseudomembranous colitis in humans (Bartlett et al Am. J. Clin. Nutr. 11 suppl:2521-6 (1980)). The overall associated mortality rate for individuals infected with  C. difficile  was calculated to be 5.99% within 3 months of diagnosis, with higher mortality associated with advanced age, being 13.5% in patients over 80 years (Karas et al Journal of Infection 561:1-9 (2010)).The current treatment for  C. difficile  infection is the administration of antibiotics (metronidazole and vancomycin), however there has been evidence of strains which are resistant to these antibiotics (Shah et al., Expert Rev. Anti Infect. Ther. 8(5), 555-564 (2010)). Accordingly there is a need for immunogenic compositions capable of inducing antibodies to, and/or a protective immune response to,  C. difficile.    
     The enterotoxicity of  C. difficile  is primarily due to the action of two toxins, toxin A and toxin B. These are both potent cytotoxins (Lyerly et al Current Microbiology 21 :29-32 (1990). 
     It has been demonstrated that fragments of toxin A, in particular fragments of the C-terminal domain, can lead to a protective immune response in hamsters (Lyerly et al Current Microbiology 21:29-32 (1990)), WO96/12802 and WO00/61762. However the present inventors have demonstrated that antibodies against toxin A and toxin B alone are not sufficient in order to prevent disease caused by certain strains, in particular serogroup 078 and 027 strains. For this reason vaccines which are capable of protecting against these strains are still required. 
     Some strains, but not all, also express the binary toxin (CDT). Similar to many other binary toxins, CDT is composed of two components—an enzymatically active component (CDTa) and a catalytically inert transport component (CDTb). The catalytically inert component facilitates translocation of the CDTa into the target cell. 
     CDTa has an ADP-ribosylating activity, which transfers the ADP-ribose moiety of NAD/NADPH to the monomeric actin (G-actin) in the target cell and thus preventing its polymerization to F-actin and resulting in disruption of the cytoskeleton and eventual cell death (Sundriyal et al, Protein expression and Purification 74 (2010) 42-48). 
     WO2013/1 12867 (Merck) describes vaccines against  Clostridium difficile  comprising recombinant  C. difficile  Toxin A and Toxin B and binary toxin A (CDTa) proteins comprising specifically defined mutations relative to the native toxin sequence that are described as substantially reducing or eliminating toxicity, in combination with binary toxin B (CDTb). 
     The present inventors have found, that binary toxin can be used to provide an improved vaccine against  C. difficile  particularly providing protection against several of the most concerning  C. difficile  strains (such as the 027 and 078 strains). Furthermore the present inventors have demonstrated, for the first time, that only CDTa or CDTb (not both) is required in order to generate antibodies which are capable of neutralizing strains expressing binary toxin. In addition the inventors have demonstrated, for the first time, that CDTa proteins comprising mutations which reduce the ADP-ribosylating activity of CDTa, are still capable of raising an immune response. In addition, the inventors have demonstrated that truncated CDTa proteins are capable of raising an immune response. Similarly the inventors have demonstrated that truncated CDTb proteins are capable of raising an immune response, that CDTb can raise an immune response when it is in its monomeric or polymeric form and that fusion proteins comprising CDTa and CDTb or CDTb fused to isolated toxin A and/or isolated toxin B are capable of raising an immune response. Finally, the inventors have demonstrated that an immunogenic composition comprising binary toxin can be improved by adding an adjuvant, in particular an adjuvant comprising an immunologically active saponin presented in the form of a liposome or an oil in water emulsion. 
     SUMMARY OF INVENTION 
     In a first aspect of the invention there is provided an immunogenic composition comprising an isolated  Clostridium difficile  CDTb protein wherein the composition does not further comprise an isolated protein having at least 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 100% similarity to SEQ ID NO: 1, SEQ ID NO: 31 or SEQ ID NO: 32. 
     In a second aspect of the invention there is provided an immunogenic composition comprising isolated  Clostridium difficile  CDTb protein wherein the isolated  Clostridium difficile  CDTb protein is a truncated CDTb protein comprising the receptor binding domain. 
     In a third aspect of the invention there is provided an immunogenic composition comprising isolated  Clostridium difficile  CDTb protein wherein the isolated  Clostridium difficile  CDTb protein is a mutated CDTb protein incapable of binding to CDTa. 
     In a fourth aspect of the invention there is provided an immunogenic composition comprising isolated  Clostridium difficile  CDTa protein wherein the isolated  Clostridium difficile  CDTa protein is a truncated CDTa protein which does not comprise the C-terminal domain. 
     In a fifth aspect the present invention provides an immunogenic composition comprising a fusion protein comprising a CDTa protein and a CDTb protein. 
     In a sixth aspect the present invention provides an immunogenic composition comprising a fusion protein between an isolated  Clostridium difficile  toxin A protein and/or an isolated  Clostridium difficile  toxin B protein fused to a CDTb protein. 
     In a seventh aspect the present invention provides a vaccine comprising the immunogenic composition of any one of the first five aspects and a pharmaceutically acceptable excipient. 
     In an eighth aspect the present invention provides the immunogenic composition of any one of the first five aspects or the vaccine of the sixth aspect, for use in the treatment or prevention of disease e.g.  C. difficile  disease. 
     In a ninth aspect the present invention provides the use of an immunogenic composition of any one of the first five aspects or the vaccine of the sixth aspect in the preparation of a medicament for the prevention or treatment of disease e.g.  C. difficile  disease. 
     In a tenth aspect the present invention provides a method of preventing or treating  C. difficile  disease comprising administering an immunogenic composition of any one of the first six aspects or the vaccine of the seventh aspect to a mammalian subject. 
     In a further aspect of the invention there is provided an immunogenic composition comprising an isolated  Clostridium difficile  CDTb protein. 
     In a further aspect of the invention there is provided an immunogenic composition comprising either an isolated  Clostridium difficile  CDTb protein or an isolated CDTa protein but does not comprise both an isolated CDTb protein and an isolated CDTa protein. 
     Novel polypeptides and nucleotides as defined herein also form further aspects of the invention. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  (comprising  FIGS. 1 a -1 h   )—Graphs describing the size distribution of the different CdtA, CdtB and CdtA-CdtB fusion constructions as determined by sedimentation velocity analytical ultracentrifugation: 
         FIG. 1   a:  AUC of C67 (CdtA (aa44-463) mut E428Q-E430Q 
         FIG. 1   b:  AUC of C69 CdtA (aa44-463) mut. R345A-Q350A-N385A-R402A-S388F-E428Q-E4300 
         FIG. 1 c   : AUC of C50 (CdtA N-term without linker (aa44-260) 
         FIG. 1 d   : AUC of C61 (fusion CdtA N term with linker-CdtBshort) 
         FIG. 1 e   : AUC of C62 (fusion CdtA N term without linker-CdtBlong) 
         FIG. 1 f   : AUC of C52 (CdtB long) 
         FIG. 1 g   : AUC of C53 (CdtB short) 
         FIG. 1 h   : AUC of C55 CdtB Δ prodomain (aa. 212-876) 
         FIG. 2  (comprising  FIGS. 2 a -2 c   )—SDS PAGE profiles of CdtA, CdtB and CdtA-CdtB fusion constructions after purification: 
         FIG. 2 a   : SDS PAGE of purified CdtA-CdtB fusion constructions. Lane 1: Molecular weight marker Novex sharp prestained. Lane 2: 5 μg of C61 CdtA N-term link (aa. 44-268)-CdtB RBD short (aa. 636-876). Lane 3: 5 μg of C62 CdtA N-term (aa. 44-260)-CdtB RBD long (aa. 621-876). 
         FIG. 2 b   : SDS PAGE of purified CdtA constructions. Lane 1: Molecular weight marker Novex sharp prestained. Lane 2: 5 μg of C50 CdtA WO linker (44-260). Lane 3: 5 μg of C67 CdtA full length (aa44-463) mut. E428Q-E430Q. Lane 4: 5 μg of C69 CdtA full length (aa44-463) mut. R345A-Q350A-N385A-R402A-S388F-E428Q-E430Q. 
         FIG. 2 c   : SDS PAGE of purified CdtB constructions. Lane 1: Molecular weight marker Novex sharp prestained. Lane 2: 5 μg of C37 CdtB&#39; Asignal sequence (aa43-876)+GST N-term after removal of the N-term GST and activation by prodomain cleavage with chymotrypsin. Lane 3: 5 μg of C55 CdtB A prodomain (aa. 212-876). Lane 4: 5 μg of C52 CdtB receptor binding domain long (aa. 621-876). Lane 5: Molecular weight marker. Lane 6: 5 μg of C38 CdtB&#39; Asignal sequence (aa43-876). 
         FIG. 3 —Graph showing anti-CDTb immunogenicity in mice immunised with  C. difficile  Binary Toxin component A or  C. difficile  Binary Toxin component B, in both cases formulated with adjuvant 
         FIG. 4 —Graph showing anti-CDTa immunogenicity in mice immunised with  C. difficile  Binary Toxin component A or  C. difficile  Binary Toxin component B, in both cases formulated with adjuvant 
         FIG. 5 —Cytotoxicity inhibition titres in HCT116 cells from mice immunised with  C. difficile  Binary Toxin component A or  C. difficile  Binary Toxin component B, in both cases formulated with adjuvant 
         FIG. 6 —Cytotoxicity inhibition titres in HT29 cells from mice immunised with  C. difficile  Binary Toxin component A or  C. difficile  Binary Toxin component B, in both cases formulated with adjuvant 
         FIG. 7 —Graph showing anti-CDTb immunogenicity in mice immunised with  C. difficile  Cdtb (activated or non activated, with and without F2 fusion comprising fragments from Toxin A and Toxin B) formulated with adjuvant 
         FIG. 8 —Graph showing anti-Tox A immunogenicity in mice immunised with  C. difficile  Cdtb (activated or non activated, with and without F2 fusion comprising fragments from Toxin A and Toxin B) formulated with adjuvant 
         FIG. 9 —Graph showing anti-Tox B immunogenicity in mice immunised with  C. difficile  Cdtb (activated or non activated, with and without F2 fusion comprising fragments from Toxin A and Toxin B) formulated with adjuvant 
         FIG. 10 —Tox A cytotoxicity inhibition titres in HT29 cells from mice immunised with  C. difficile  Cdtb (activated or non activated, with and without F2 fusion comprising fragments from Toxin A and Toxin B) formulated with adjuvant 
         FIG. 11 —Tox B cytotoxicity inhibition titres in HCT116 cells from mice immunised with  C. difficile  Cdtb (activated or non activated, with and without F2 fusion comprising fragments from Toxin A and Toxin B) formulated with adjuvant 
         FIG. 12 —Binary Toxin cytotoxicity inhibition titres in HT29 cells from mice immunised with  C. difficile  Binary Toxin component A or  C. difficile  Binary Toxin component B, in both cases formulated with adjuvant 
         FIG. 13 —Graph showing anti-CDTb immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 6 μg/dose in an adjuvant formulation 
         FIG. 14 —Graph showing anti-CDTa immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 6 μg/dose in an adjuvant formulation 
         FIG. 15 —Graph showing anti-Tox B immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 6 μg/dose in an adjuvant formulation 
         FIG. 16 —Graph showing anti-Tox A immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 6 μg/dose in an adjuvant formulation 
         FIG. 17 —Binary Toxin cytotoxicity inhibition titres in HCT116 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 6 μg/dose in an adjuvant formulation 
         FIG. 18 —Binary Toxin cytotoxicity inhibition titres in HT29 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 6 μg/dose in an adjuvant formulation 
         FIG. 19 —Tox A cytotoxicity inhibition titres in HT29 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 6 μg/dose in an adjuvant formulation 
         FIG. 20 —Tox B cytotoxicity inhibition titres in HCT116 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 6 μg/dose in an adjuvant formulation 
         FIG. 21 —Graph showing anti-CDTb immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 2 μg/dose in an adjuvant formulation 
         FIG. 22 —Graph showing anti-CDTa immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 2 μg/dose in an adjuvant formulation 
         FIG. 23 —Graph showing anti-Tox B immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 2 μg/dose in an adjuvant formulation 
         FIG. 24 —Graph showing anti-Tox A immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 2 μg/dose in an adjuvant formulation 
         FIG. 25 —Binary Toxin cytotoxicity inhibition titres in HCT116 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 2 μg/dose in an adjuvant formulation 
         FIG. 26 —Binary Toxin cytotoxicity inhibition titres in HT29 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 2 μg/dose in an adjuvant formulation 
         FIG. 27 —Tox A cytotoxicity inhibition titres in HT29 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 2 μg/dose in an adjuvant formulation 
         FIG. 28 —Tox B cytotoxicity inhibition titres in HCT116 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 2 μg/dose in an adjuvant formulation 
         FIG. 29 —Graph showing anti-CDTb immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 10 μg/dose in a non-adjuvanted formulation 
         FIG. 30 —Graph showing anti-CDTa immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 10 μg/dose in a non-adjuvanted formulation 
         FIG. 31 —Graph showing anti-Tox B immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 10 μg/dose in a non-adjuvanted formulation 
         FIG. 32 —Graph showing anti-Tox A immunogenicity in mice immunized with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 10 μg/dose in a non-adjuvanted formulation 
         FIG. 33 —Binary Toxin cytotoxicity inhibition titres in HCT116 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 10 μg/dose in a non-adjuvanted formulation 
         FIG. 34 —Binary Toxin cytotoxicity inhibition titres in HT29 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 10 μg/dose in a non-adjuvanted formulation 
         FIG. 35 —Tox A cytotoxicity inhibition titres in HT29 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 10 μg/dose in a non-adjuvanted formulation 
         FIG. 36 —Tox B cytotoxicity inhibition titres in HCT116 cells from mice immunised with different binary toxin vaccine candidates (CdtA/CdtB) combined with F2 at 10 μg/dose in a non-adjuvanted formulation 
     
    
    
     DETAILED DESCRIPTION 
     Binary Toxin 
     The  Clostridium difficile  binary toxin comprises two different proteins, CDTa and CDTb. During infection CDTb is activated by proteolytic cleavage by a chymotrypsin-like protease to produce a CDTb protein lacking the prodomain (also referred to as CDTb″). Note that CDTb″ also lacks the CDTb signal sequence, a CDTb protein lacking the signal sequence but not lacking the prodomain is referred to as CDTb′. After proteolytic activation the CDTb oligomerises and binds to CDTa to form the complete ‘binary toxin’. The binding of the binary toxin to the cell receptors leads to receptor-mediated endocytosis. As the endosome acidifies the CDTb binding domain undergoes conformational changes that allow the CDTb oligomer to form a pore, the pore formation triggers translocation of the ADP-ribosyltransferase domain (CDTa) into the target cell. 
     CDTb 
     The present invention provides an immunogenic composition comprising an isolated  Clostridium difficile  CDTb protein. The present invention also provides an immunogenic composition comprising an isolated  Clostridium difficile  CDTb protein as the sole  C. difficile  antigen. As used herein the term “as the sole  C. difficile  antigen” means that the immunogenic composition comprising an isolated  Clostridium difficile  CDTb protein as the sole  C. difficile  antigen does not also comprise another antigen from  C. difficile  e.g. the immunogenic composition does not also comprise a toxin A, toxin B or CDTa protein. 
     The present invention provides an immunogenic composition comprising an isolated  Clostridium difficile  CDTb protein wherein the composition does not further comprise an isolated protein having at least 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% or 100% similarity to SEQ ID NO: 1, SEQ ID NO: 31 or SEQ ID NO: 32. According to the invention as herein described the term ‘CDTb protein’ encompasses SEQ ID NO:3 or fragments or variants of SEQ ID NO:3. 
     In one embodiment of this first aspect of the invention, the composition does not comprise an isolated  Clostridium difficile  CDTa protein. 
     In one embodiment of this aspect the isolated  Clostridium difficile  CDTb protein is or comprises 
     (i) SEQ ID NO: 3; or 
     (ii) a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO:3; or 
     (iii) a fragment of CDTb having at least 30, 50, 80, 100, 120, 150, 200, 250 or 300 contiguous amino acids of SEQ ID NO:3. 
     In one such aspect there is provided an immunogenic composition wherein the isolated  Clostridium difficile  CDTb protein is a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO:3. 
     In another aspect there is provided an immunogenic composition wherein the isolated  Clostridium difficile  CDTb protein is a fragment of CDTb having at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750. 800 or 850 contiguous amino acids of SEQ ID NO: 3. 
     CDTb comprises multiple domains, in particular CDTb comprises a signal peptide and a prodomain both of which are cleaved as explained in the section entitled “Binary Toxin” above. 
     In one embodiment the isolated  Clostridium difficile  CDTb protein is a truncated CDTb protein with the signal peptide removed. The term ‘truncated CDTb protein with the signal peptide removed’ refers to a fragment or variant of SEQ ID NO: 3 with substantially all of the signal peptide removed (therefore which does not comprise amino acids corresponding to substantially all of the signal peptide), there may be a few amino acids of the signal peptide remaining, for example 2, 5, 10, 15 or 20 amino acids of the signal peptide may remain. The signal peptide corresponds to amino acids 1-48 (encompassing amino acids 1-42) of SEQ ID NO: 3 or their equivalents in a binary toxin protein isolated from a different strain of  C. difficile,  for example amino acids 1-42 of the amino acid sequence of CDTb from strain CD196 (Perelle, M. et al Infect. Immun., 65 (1997), pp. 1402-1407). 
     Suitably in this embodiment the isolated  Clostridium difficile  CDTb protein is or comprises 
     (i) SEQ ID NO: 7 or SEQ ID NO: 16; or 
     (ii) a variant of CDTb having at least 80%, 85%, 88%, 90%. 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 7 or SEQ ID NO:16; or 
     (iii) a fragment of CDTb having at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750 or 800 contiguous amino acids of SEQ ID NO: 7 or SEQ ID NO:16. In one embodiment the truncated CDTb protein with the signal peptide removed is or comprises a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 7 or SEQ ID NO:16. In a further embodiment the isolated truncated CDTb protein with the signal peptide removed is or comprises a fragment of CDTb having at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, or 800 contiguous amino acids of SEQ ID NO: 7 or SEQ ID NO:16. 
     In one embodiment the isolated  Clostridium difficile  CDTb protein is a truncated CDTb protein with the prodomain removed. The term ‘truncated CDTb protein with the prodomain removed’ refers to a fragment or variant of SEQ ID NO: 3 with substantially all of the prodomain removed (therefore which does not comprise amino acids corresponding to substantially all of the prodomain), there may be a few amino acids of the prodomain remaining, for example 2, 5, 10, 15 or 20 amino acids of the prodomain may remain. The prodomain corresponds to amino acids 48-211 (encompassing amino acids 48-166) of SEQ ID NO:3 or their equivalents in a binary toxin protein isolated from a different strain of  C. difficile.  Optionally the truncated CDTb protein with the prodomain removed also lacks the CDTb signal sequence, the CDTb signal sequence corresponds to amino acids 1-48 (encompassing amino acids 1-42) of SEQ ID NO:3 or their equivalents in a different strain. The term ‘truncated CDTb protein with the prodomain removed’ may also refer to a fragment or variant of SEQ ID NO: 3 which is capable of oligomerising and binding to CDTa. In this embodiment of the invention the isolated  Clostridium difficile  CDTb protein suitably is or comprises 
     (i) SEQ ID NO: 9 or SEQ ID NO: 51; or 
     (ii) a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO:9 or SEQ ID NO: 51; or 
     (iii) a fragment of CDTb having at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600 or 650 contiguous amino acids of SEQ ID NO:9 or SEQ ID NO: 51. 
     In one embodiment the truncated CDTb protein with the prodomain removed is or comprises a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO:9. In a further embodiment the isolated truncated CDTb protein with the prodomain removed is or comprises a fragment of CDTb having at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, or 800 contiguous amino acids of SEQ ID NO:9. 
     CDTb also comprises a receptor binding domain. In one embodiment the isolated  Clostridium difficile  CDTb protein is a truncated CDTb protein comprising the receptor binding domain. The term ‘truncated CDTb protein comprising the receptor binding domain’ refers to a fragment or variant of SEQ ID NO:3 with substantially all but the receptor binding domain removed (therefore which does not comprise amino acids corresponding to substantially all of the protein except for the receptor binding domain), there may be a few amino acids in addition to the receptor binding domain remaining, for example 2, 5, 10, 15 or 20 amino acids except for/in addition to the receptor binding domain. In one version, the receptor binding domain corresponds to amino acids 620-876 of SEQ ID NO:3, or their equivalents in a binary toxin protein isolated from a different strain of  C. difficile.  In another version, the receptor binding domain corresponds to amino acids 636-876 of SEQ ID NO:3 or their equivalents in a binary toxin protein isolated from a different strain of  C. difficile.    
     In this embodiment the isolated  Clostridium difficile  CDTb protein suitably is or comprises 
     (i) SEQ ID NO: 34 or SEQ ID NO: 36; or 
     (i) a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 34 or SEQ ID NO: 36; or 
     (iii) a fragment of CDTb having at least 30, 50, 80, 100, 120, 150 or 200 contiguous amino acids of SEQ ID NO: 34 or SEQ ID NO: 36. 
     In another embodiment of this aspect of the invention, the isolated  Clostridium difficile  CDTb protein is a mutated CDTb protein incapable of binding to CDTa. 
     In this embodiment the isolated  Clostridium difficile  CDTb protein suitably is or comprises 
     (i) SEQ ID NO: 50; or 
     (i) a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 50; or 
     (iii) a fragment of CDTb having at least 30, 50, 80, 100, 120, 150, 200, 250 or 300 contiguous amino acids of SEQ ID NO: 50. 
     The CDTb protein varies in amino acid sequence between different strains, for this reason the amino acid numbering may differ between strains. For this reason the term ‘equivalents in a different strain’ refers to amino acids which correspond to those of a reference strain (e.g.,  C. difficile  R20291 from which SEQ ID NO:1 and SEQ ID NO:3 are derived), but which are found in a toxin from a different strain and which may thus be numbered differently. A region of ‘equivalent’ amino acids may be determined by aligning the sequences of the toxins from the different strains. Example binary toxin producing strains of  C. difficile  include CD196, CCUG 20309, R8637, IS81, IS93, IS51, IS58, R6786, R7605, R10456 and R5989. The amino acids numbers provided throughout refer to those of reference strain R20291. 
     In one embodiment the isolated  Clostridium difficile  CDTb protein is a monomer of CDTb. In a further embodiment the isolated  Clostridium difficile  CDTb protein is a multimer of CDTb. 
     In a further embodiment the isolated  Clostridium difficile  CDTb protein is a heptamer of CDTb. 
     In a second aspect the present invention provides immunogenic compositions comprising isolated  Clostridium difficile  CDTb protein wherein the isolated  Clostridium difficile  CDTb protein is a truncated CDTb protein comprising the receptor binding domain. In one embodiment of this aspect the isolated  Clostridium difficile  CDTb protein suitably is or comprises 
     (i) SEQ ID NO: 34 or SEQ ID NO: 36; or 
     (i) a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 34 or SEQ ID NO: 36; or 
     (iii) a fragment of CDTb having at least 30. 50, 80, 100, 120, 150 or 200 contiguous amino acids of SEQ ID NO: 34 or SEQ ID NO: 36. 
     In a third aspect the invention provides immunogenic compositions comprising isolated  Clostridium difficile  CDTb protein wherein the isolated  Clostridium difficile  CDTb protein is a mutated CDTb protein incapable of binding to CDTa. In one embodiment of this aspect, the isolated  Clostridium difficile  CDTb protein suitably is or comprises 
     (i) SEQ ID NO: 50; or 
     (i) a variant of CDTb having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 50; or 
     (iii) a fragment of CDTb having at least 30, 50, 80, 100, 120, 150. 200, 250 or 300 contiguous amino acids of SEQ ID NO: 50. 
     In one embodiment of the second and third aspects of the invention, the immunogenic composition comprises/further comprises an isolated  Clostridium difficile  CDTa protein comprising 
     (i) SEQ ID NO: 1; or 
     (ii) a variant of CDTa having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%. 99%, 100% sequence identity to SEQ ID NO:1; or 
     (iii) a fragment of CDTa having at least at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350 or 400 contiguous amino acids of SEQ ID NO:1. 
     CDTa 
     The present invention also provides immunogenic compositions comprising an isolated  Clostridium difficile  CDTa protein. The present invention also provides immunogenic compositions comprising an isolated  Clostridium difficile  CDTa protein as the sole  C. difficile  antigen. As used herein the term “as the sole  C. difficile  antigen” means that the immunogenic composition comprising an isolated  Clostridium difficile  CDTa protein as the sole  C. difficile  antigen does not also comprise another antigen from  C. difficile  e.g. the immunogenic composition does not also comprise a toxin A, toxin B or CDTb protein. According to the invention as herein described the term ‘CDTa protein’ encompasses SEQ ID NO:1 or fragments or variants of SEQ ID NO:1. In one embodiment the isolated  Clostridium difficile  CDTa protein comprises a variant of CDTa having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO:1. In a further embodiment the isolated  Clostridium difficile  CDTa protein comprises a fragment of CDTa having at least at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350 or 400 contiguous amino acids of SEQ ID NO:1. 
     CDTa comprises two domains, the C-terminal domain is responsible for the ADP ribosyltransferase activity whilst the N-terminal domain is responsible for interacting with CDTb. 
     In one embodiment of the first three aspects of the invention, the immunogenic composition comprises/further comprises an isolated  Clostridium difficile  CDTa protein. Suitably the isolated  Clostridium difficile  CDTa protein is a truncated CDTa protein. “A truncated CDTa protein” as used herein means a CDTa protein that does not achieve its full length or its proper form, and thus is missing some of the amino acid residues that are present in full length CDTa of SEQ ID NO: 1, and which cannot perform the function for which it was intended because its structure is incapable of doing so, e.g. ADP ribosyltransferase activity and/or interacting with CDTb. 
     Suitably the isolated  Clostridium difficile  CDTa protein is a truncated CDTa protein which does not comprise the C-terminal domain. The term ‘truncated CDTa protein which does not comprise the C-terminal domain’ refers to a fragment or variant of SEQ ID NO:1 which does not comprise a substantial portion of the C-terminal domain, there may be a few amino acids of the C-terminal domain remaining, for example, 2, 5, 10, 15, 20, 25, 30, 35 or 50 amino acids of the C-terminal domain may remain. The C-terminal domain corresponds to amino acids 267-463 of SEQ ID NO:1 or their equivalents in a CDTa protein isolated from a different strain of  C. difficile.  In this embodiment the truncated  Clostridium difficile  CDTa protein suitably is or comprises 
     (i) SEQ ID NO: 14 or SEQ ID NO: 15 
     (i) a variant of CDTa having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 14 or SEQ ID NO: 15; or 
     (iii) a fragment of CDTa having at least 30, 50, 80, 100, 120, 150, or 190 contiguous amino acids of SEQ ID NO: 14 or SEQ ID NO: 15. 
     In one embodiment the truncated CDTa protein which does not comprise the C-terminal domain is a variant of CDTa having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO:13. In a further embodiment the truncated CDTa protein which does not comprise the C-terminal domain is a variant of CDTa having at least 30, 50, 80, 100, 120, 150, or 190 contiguous amino acids of SEQ ID NO:13. 
     In a fourth aspect the invention provides an immunogenic composition comprising isolated  Clostridium difficile  CDTa protein wherein the isolated  Clostridium difficile  CDTa protein is a truncated CDTa protein which does not comprise the C-terminal domain. In one embodiment of this aspect, the isolated  Clostridium difficile  CDTa protein suitably is or comprises 
     (i) SEQ ID NO: 14 or SEQ ID NO: 15; or 
     (ii)a variant of CDTa having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO:14 or SEQ ID NO: 15; or 
     (iii) a fragment of CDTa having at least 30, 50, 80, 100, 120, 150, or 190 contiguous amino acids of SEQ ID NO:14 or SEQ ID NO: 15. 
     In a further embodiment of any of the aspects of the invention, the isolated  Clostridium difficile  CDTa protein suitably contains a mutation which reduces its ADP-ribosyltransferase activity. For example the isolated  Clostridium difficile  CDTa protein has a mutation from glutamate to another amino acid at position 428. The term ‘has a mutation at position 428’ refers to CDTa proteins which have a mutation at this exact location but also to a CDTa protein which is isolated from a different strain and which has a mutation at an equivalent position. The CDTa protein varies in amino acid sequence between different strains, for this reason the amino acid numbering may differ between strains, thus a CDTa protein from a different strain may have a corresponding glutamate which is not number 428 in sequence. In one embodiment the isolated  Clostridium difficile  CDTa protein has a mutation from glutamate to glutamine at position 428. 
     In a further embodiment of any of the aspects of the invention, the isolated  Clostridium difficile  CDTa protein suitably has a mutation from glutamate to a different amino acid at position 430, the term ‘has a mutation at position 430’ refers to proteins which have this exact location but also to a CDTa protein which is isolated from a different strain and which has a mutation at an equivalent position. In one embodiment the isolated  Clostridium difficile  CDTa protein has a mutation from glutamate to glutamine at position 430. 
     In a further embodiment of any of the aspects of the invention, the isolated  Clostridium difficile  CDTa protein suitably is or comprises 
     (i) SEQ ID NO: 46; SEQ ID NO: 48; SEQ ID NO: 52; or SEQ ID NO: 54; or 
     (ii) a variant of CDTa having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 46; SEQ ID NO: 48; SEQ ID NO: 52; or SEQ ID NO: 54; or 
     (iii) a fragment of CDTa having at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350 or 400 contiguous amino acids of SEQ ID ID NO: 46; SEQ ID NO: 48; SEQ ID NO: 52; or SEQ ID NO: 54. 
     In a further embodiment of any of the aspects of the invention, the isolated  Clostridium difficile  CDTa protein suitably is or comprises 
     (i) SEQ ID NO: 48; or 
     (ii) a variant of CDTa having at least 80%. 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 48; or 
     (iii) a fragment of CDTa having at least 30, 50, 80, 100, 120, 150, 200, 250, 300, 350 or 400 contiguous amino acids of SEQ ID ID NO: 48. 
     Immunogenic Compositions with CDTa and/or CDTb 
     In a further embodiment there is provided an immunogenic composition which comprises a CDTb protein but does not comprise a CDTa protein, for example the immunogenic composition does not comprise a variant of CDTa having at least 95%, 98%, 99%, or 100% sequence identity to SEQ ID NO:1 or a fragment of CDTa having at least at least 250, 400 or 450. contiguous amino acids of SEQ ID NO:1. 
     In a further embodiment there is provided an immunogenic composition which comprises a CDTa protein but does not comprise a CDTb protein, for example the immunogenic composition does not comprise a variant of CDTb having at least 95%, 98%, 99%, or 100% sequence identity to SEQ ID NO:3 or a fragment of CDTb having at least 700, 750, or 800 contiguous amino acids of CDTb. 
     In a further embodiment there is provided an immunogenic composition which comprises either an isolated  Clostridium difficile  CDTb protein or an isolated CDTa protein but does not comprise both an isolated CDTb protein and an isolated CDTa protein. 
     In a further embodiment there is provided a fusion protein comprising a CDTa protein and a CDTb protein. In another embodiment there is provided immunogenic compositions comprising a fusion protein comprising a CDTa protein and a CDTb protein. 
     Fusion Proteins Comprising a CDTa Protein and a CDTb Protein 
     In a fifth aspect, the invention provides immunogenic compositions comprising a fusion protein comprising a CDTa protein and a CDTb protein. In one embodiment of this aspect, the CDTa protein suitably is truncated. For example, the CDTa protein suitably does not comprise the C-terminal domain. In this aspect, the CDTb protein suitably is truncated. In this embodiment, the CDTb protein suitably comprises the receptor binding domain. 
     In one embodiment of this aspect of the invention, the fusion protein suitably is or comprises 
     (i) SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; or SEQ ID NO: 43; or 
     (ii) a variant having at least 80%, 85%, 88%, 90%, 92%, 95%, 98%, 99%, 100% sequence identity to SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; or SEQ ID NO: 43; or 
     (iii) a fragment having at least 30, 50, 80, 100, 120, 150. 200, 250, 300, 350 or 400 contiguous amino acids of SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; or SEQ ID NO: 43. 
     “Fusion polypeptide” or “fusion protein” refers to a protein having at least two heterologous polypeptides (e.g. at least two Mycobacterium sp. polypeptides) covalently linked, either directly or via an amino acid linker. It may also refer to a protein having at least two heterologous polypeptides linked non-covalently. The polypeptides forming the fusion protein are typically linked C-terminus to N-terminus, although they can also be linked C-terminus to C-terminus, N-terminus to N-terminus, or N-terminus to C-terminus. The polypeptides of the fusion protein can be in any order. This term also refers to conservatively modified variants, polymorphic variants, alleles, mutants, immunogenic fragments, and interspecies homologs of the antigens that make up the fusion protein. 
     The term “fused” refers to the linkage e.g. covalent linkage between two polypeptides in a fusion protein. The polypeptides are typically joined via a peptide bond, either directly to each other or via an amino acid linker. Optionally, the peptides can be joined via non-peptide covalent linkages known to those of skill in the art. 
     A peptide linker sequence may be employed to separate the first and second polypeptide components by a distance sufficient to ensure that each polypeptide folds into its secondary and tertiary structures. Such a peptide linker sequence is incorporated into the fusion protein using standard techniques well known in the art. Suitable peptide linker sequences may be chosen based on the following factors: (1) their ability to adopt a flexible extended conformation; (2) their inability to adopt a secondary structure that could interact with functional epitopes on the first and second polypeptides; and (3) the lack of hydrophobic or charged residues that might react with the polypeptide functional epitopes. Preferred peptide linker sequences contain Gly, Asn and Ser residues. Other near neutral amino acids, such as Thr and Ala may also be used in the linker sequence. Amino acid sequences which may be usefully employed as linkers include those disclosed in Maratea et al., Gene 40:39-46 (1985); Murphy et al., Proc. Natl. Acad. Sci. USA 83:8258-8262 (1986); U.S. Pat. No. 4,935,233 and U.S. Pat. No. 4,751,180. The linker sequence may generally be from 1 to about 50 amino acids in length for example 1, 5, 10, 15, 20, 25, 30, 35 or 40 amino acids in length. Linker sequences are not required when the first and second polypeptides have non-essential N-terminal amino acid regions that can be used to separate the functional domains and prevent steric interference. 
     In one embodiment of any of the aspects of the invention, the immunogenic composition elicits antibodies that neutralize CDTa or CDTb or both. In a further embodiment the composition elicits antibodies that neutralize binary toxin. Whether a composition elicits antibodies against a toxin can be measured by immunising mice with the immunogenic composition, collecting sera and analysing the anti-toxin titres of the sera using by ELISA. The sera should be compared to a reference sample obtained from mice which have not been immunised. The composition of the invention elicits antibodies that neutralise CDTa if the sera against the polypeptide gives an ELISA readout more than 10%, 20%, 30%, 50%, 70%, 80%, 90%, or 100% higher than the reference sample. 
     In a further embodiment the immunogenic compositions of the invention elicits a protective immune response in a mammalian host against strains of  C. difficile.  In one embodiment the mammalian host is selected from the group consisting of mouse, rabbit, guinea pig, non-human primate, monkey and human. In one embodiment the mammalian host is a mouse. In a further embodiment the mammalian host is a human. 
     Whether an immunogenic composition elicits a protective immune response in a mammalian host against strains of  C. difficile  can be determined using a challenge assay. In such an assay the mammalian host is vaccinated with the immunogenic composition and challenged by exposure to  C. difficile,  the time which the mammal survives after challenge is compared with the time which a reference mammal that has not been immunised with the immunogenic composition survives. An immunogenic composition elicits a protective immune response if a mammal immunised with the immunogenic composition survives at least 10%, 20%, 30%, 50%, 80%, 80%, 90%, or 100% longer than a reference mammal which has not been immunised after challenge with  C. difficile.    
     Toxin A and Toxin B 
     In one embodiment of any of the aspects of the invention, the immunogenic compositions of the invention further comprise an isolated  Clostridium difficile  toxin A protein and/or an isolated  C. difficile  toxin B protein. 
     The term ‘isolated  Clostridium difficile  toxin A protein’ refers to a fragment or variant of SEQ ID NO: 31. In one embodiment the isolated  Clostridium difficile  toxin A protein is a fragment comprising 50, 100, 150, 200, 250, 300, 500, 750, 1000, 1250, 1500, 1750, 2000 or 2500 contiguous amino acids of SEQ ID NO:31 . In one embodiment the isolated  Clostridium difficile  toxin A protein is a variant comprising 80%, 85%, 90%, 92%, 95%, 98%, 99% or 100% identity to SEQ ID NO:31. 
     The term ‘isolated Clostridiurn difficile toxin B protein’ refers to a fragment or variant of SEQ ID NO: 32. In one embodiment the isolated  Clostridium difficile  toxin B protein is a fragment comprising 50, 100, 150, 200, 250, 300, 500, 750, 1000, 1250, 1500, 1750 or 2000 SEQ ID NO:32 . In one embodiment the isolated  Clostridium difficile  toxin B protein is a variant comprising 80%, 85%, 90%, 92%, 95%, 98%, 99% or 100% identity to SEQ ID NO:32. 
     In one embodiment the isolated  Clostridium difficile  toxin A protein comprises a repeating domain fragment. The term ‘toxin A repeating domain’ refers to the C-terminal domain of the toxin A protein from  C. difficile,  comprising repeated sequences. The toxin A repeating domain refers to amino acids 1832-2710 of toxin A from strain VP110463 (ATCC43255) and their equivalents in a different strain, the sequence of amino acids 1832-2710 from strain VP110463 (ATCC43255) corresponds to amino acids 1832-2710 of SEQ ID NO: 31. In a further embodiment the isolated  Clostridium difficile  toxin A protein comprises a fragment of the toxin A N-terminal domain. The toxin A N-terminal domain refers to amino acids 1-1831 of toxin A from strain VB110463 (ATCC43255) and their equivalents in a different strain, the sequence of amino acids 1-1831 of SEQ ID NO: 31. 
     In one embodiment the isolated  Clostridium difficile  toxin B protein comprises a toxin B repeating domain fragment. The term ‘toxin B repeating domain’ refers to the C-terminal domain of the toxin B protein from  C. difficile.  This domain refers to amino acids 1834-2366 from strain VP110463 (ATCC43255) and their equivalents in a different strain, the sequence of amino acids 1834-2366 from strain VP110463 (ATCC43255) corresponds to amino acids 1834-2366 of SEQ ID NO: 32. In a further embodiment the isolated  Clostridium difficile  toxin B protein comprises a fragment of the toxin B N-terminal domain. The toxin B N-terminal domain refers to amino acids 1-1833 of toxin B from strain VB110463 (ATCC43255) and their equivalents in a different strain, the sequence of amino acids 1-1833 of SEQ ID NO: 32. 
     The  C. difficile  toxins A and B are conserved proteins, however the sequence differs a small amount between strains, moreover the amino acid sequence for toxins A and B in different strains may differ in number of amino acids. 
     For these reasons the terms toxin A repeating domain and/or toxin B repeating domain to refer to a sequence which is a variant with 90%, 95%, 98%, 99% or 100% sequence identity to amino acids 1832-2710 of SEQ ID NO: 31 or a variant with 90%, 95%, 98%, 99% or 100% sequence identity to amino acids 1834-2366 of SEQ ID NO:32. Similarly the terms toxin a N-terminal domain and/or toxin B N terminal domain refer to a sequence which is avariant with 90%, 95%, 98%, 99% or 100% sequence identity to amino acids 1-1831 of SEQ ID NO:31 or a variant with 90%, 95%, 98%. 99% or 100% sequence identity to amino acids 1-1833 of SEQ ID NO:32. 
     Furthermore the amino acid numbering may differ between the C-terminal domains of toxin A (or toxin B) from one strain and toxin A (or toxin B) from another strain. For this reason the term ‘equivalents in a different strain’ refers to amino acids which correspond to those of a reference strain (e.g.,  C. difficile  VP110463), but which are found in a toxin from a different strain and which may thus be numbered differently. A region of ‘equivalent’ amino acids may be determined by aligning the sequences of the toxins from the different strains. The amino acids numbers provided throughout refer to those of strain VPI10463. 
     In a further embodiment of any of the aspects of the invention, the isolated  C. difficile  toxin A protein and the isolated  C. difficile  toxin B protein form a fusion protein. In one embodiment the fusion protein is 80%, 85%, 90%, 95%, 98%, 99% or 100% identical to a sequence selected from the group consisting of SEQ ID NO: 18, 19, 20, 21, 22, 24, 26, 28 and 30. In a further embodiment the fusion protein is a fragment of at least 800, 850, 900 or 950 contiguous amino acids of a sequence selected from the group consisting of SEQ ID NO: 18, 19, 20, 21, 22, 24, 26, 28 and 30. 
     In a further embodiment of any of the aspects of the invention the immunogenic composition comprises/further comprises a fusion protein between an isolated  Clostridium difficile  toxin A protein and/or an isolated  Clostridium difficile  toxin B protein fused to a CDTb protein or to a truncated CDTb protein. In one embodiment there is provided a fusion protein comprising a fragment of toxin A, a fragment of toxin B and a CDTb protein, for example the fusion protein may comprise a fragment or variant of SEQ ID NO:18, 19, 20, 21, 22, 24, 26, 28 or 30 fused to a CDTb protein. For example the fusion protein may comprise a fragment or variant of SEQ ID NO:18, 19, 20, 21, 22, 24, 26, 28 or 30 fused to a truncated CDTb protein. 
     In one embodiment the fusion protein suitably is or comprises 
     (i)SEQ ID NO: 44 or SEQ ID NO: 45; or 
     (ii) a variant having at least 80%, 85%, 90%, 95%, 98%, 99% or 100% sequence identity to SEQ ID NO: 44 or SEQ ID NO: 45; or 
     (iii) a fragment of at least 800, 850, 900 or 950 contiguous amino acids of a sequence selected from the group consisting of SEQ ID NO: 44 or SEQ ID NO: 45. 
     Fragments 
     The term “fragment” as defined herein may refer to a fragment comprising a T cell epitope. T cell epitopes are short contiguous stretches of amino acids which are recognised by T cells (e.g. CD4+ or CD8+ T cells). Identification of T cell epitopes may be achieved through epitope mapping experiments which are well known to the person skilled in the art (see. for example, Paul,  Fundamental Immunology.  3rd ed., 243-247 (1993); Beiβbarth et al  Bioinformatics  2005 21(Suppl. 1):i29-i37). 
     Suitably the fragments of the invention are immunogenic fragments. “Immunogenic fragments” according to the present invention will typically comprise at least 9 contiguous amino acids from the full length polypeptide sequence (e.g. at least 10), such as at least 12 contiguous amino acids (e.g. at least 15 or at least 20 contiguous amino acids), in particular at least 50 contiguous amino acids, such as at least 100 contiguous amino acids (for example at least 200 contiguous amino acids). Suitably the immunogenic fragments will be at least 20%, such as at least 50%, at least 70% or at least 80% of the length of the full length polypeptide sequence. 
     It will be understood that in a diverse out-bred population, such as humans, different HLA types mean that specific epitopes may not be recognised by all members of the population. Consequently, to maximise the level of recognition and scale of immune response to a polypeptide, it is generally desirable that an immunogenic fragment contains a plurality of the epitopes from the full length sequence (suitably all epitopes). 
     Variants 
     “Variants” or “conservatively modified variants” applies to both amino acid and nucleic acid sequences. With respect to particular nucleic acid sequences, conservatively modified variants refers to those nucleic acids which encode identical or essentially identical amino acid sequences, or where the nucleic acid does not encode an amino acid sequence, to essentially identical sequences. 
     In respect of variants of a protein sequence, the skilled person will recognise that individual substitutions, deletions or additions to polypeptide, which alters, adds or deletes a single amino acid or a small percentage of amino acids is a “conservatively modified variant” where the alteration(s) results in the substitution of an amino acid with a functionally similar amino acid or the substitution/deletion/addition of residues which do not substantially impact the biological function of the variant. 
     Conservative substitution tables providing functionally similar amino acids are well known in the art. Such conservatively modified variants are in addition to and do not exclude polymorphic variants, interspecies homologs, and alleles of the invention. 
     A polypeptide of the invention (such as a CDTa protein or a CDTb protein) may contain a number of conservative substitutions (for example, 1-50. such as 1-25, in particular 1-10, and especially 1 amino acid residue(s) may be altered) when compared to the reference sequence. In general, such conservative substitutions will fall within one of the amino-acid groupings specified below, though in some circumstances other substitutions may be possible without substantially affecting the immunogenic properties of the antigen. The following eight groups each contain amino acids that are typically conservative substitutions for one another: 
     1) Alanine (A), Glycine (G); 
     2) Aspartic acid (D), Glutamic acid (E); 
     3) Asparagine (N), Glutamine (Q); 
     4) Arginine (R), Lysine (K); 
     5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); 
     6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); 
     7) Serine (S), Threonine (T); and 
     8) Cysteine (C), Methionine (M) 
     (see, e.g., Creighton, Proteins 1984). 
     Suitably such substitutions do not occur in the region of an epitope, and do not therefore have a significant impact on the immunogenic properties of the antigen. 
     Polypeptide variants may also include those wherein additional amino acids are inserted compared to the reference sequence, for example, such insertions may occur at 1-10 locations (such as 1-5 locations, suitably 1 or 2 locations, in particular 1 location) and may, for example, involve the addition of 50 or fewer amino acids at each location (such as 20 or fewer, in particular 10 or fewer, especially 5 or fewer). Suitably such insertions do not occur in the region of an epitope, and do not therefore have a significant impact on the immunogenic properties of the antigen. One example of insertions includes a short stretch of histidine residues (e.g. 2-6 residues) to aid expression and/or purification of the antigen in question. 
     Polypeptide variants include those wherein amino acids have been deleted compared to the reference sequence, for example, such deletions may occur at 1-10 locations (such as 1-5 locations, suitably 1 or 2 locations, in particular 1 location) and may, for example, involve the deletion of 50 or fewer amino acids at each location (such as 20 or fewer, in particular 10 or fewer, especially 5 or fewer). Suitably such deletions do not occur in the region of an epitope, and do not therefore have a significant impact on the immunogenic properties of the antigen. 
     The skilled person will recognise that a particular polypeptide variant may comprise substitutions, deletions and additions (or any combination thereof). 
     Variants preferably exhibit at least about 70% identity, more preferably at least about 80% identity and most preferably at least about 90% identity (such as at least about 95%, at least about 98% or at least about 99%) to the associated reference sequence. 
     The terms “identical” or percent “identity,” in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or sub-sequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same (i.e., 70% identity, optionally 75%, 80%, 85%, 90%, 95%, 98% or 99% identity over a specified region), when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using, for example, one of the following sequence comparison algorithms or by manual alignment and visual inspection. Such sequences are then said to be “substantially identical.” This definition also refers to the compliment of a test sequence. Optionally, the identity exists over a region that is at least about 25 to about 50 amino acids or nucleotides in length, or optionally over a region that is 75-100 amino acids or nucleotides in length. Suitably, the comparison is performed over a window corresponding to the entire length of the reference sequence. 
     For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters. 
     A “comparison window”, as used herein, references to a segment in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequences for comparison are well-known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith &amp; Waterman,  Adv. Appl. Math.  2:482 (1981), by the homology alignment algorithm of Needleman &amp; Wunsch,  J. Mol. Biol.  48:443 (1970), by the search for similarity method of Pearson &amp; Lipman,  Proc. Nat&#39;l. Acad. Sci. USA  85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by manual alignment and visual inspection (see, e.g.,  Current Protocols in Molecular Biology  (Ausubel et al., eds. 1995 supplement)). 
     One example of a useful algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pairwise alignments to show relationship and percent sequence identity. It also plots a tree or dendogram showing the clustering relationships used to create the alignment. PILEUP uses a simplification of the progressive alignment method of Feng &amp; Doolittle,  J. Mol. Evol.  35:351-360 (1987). The method used is similar to the method described by Higgins &amp; Sharp,  CABIOS  5:151-153 (1989). The program can align up to 300 sequences, each of a maximum length of 5,000 nucleotides or amino acids. The multiple alignment procedure begins with the pairwise alignment of the two most similar sequences, producing a cluster of two aligned sequences. This cluster is then aligned to the next most related sequence or cluster of aligned sequences. Two clusters of sequences are aligned by a simple extension of the pairwise alignment of two individual sequences. The final alignment is achieved by a series of progressive, pairwise alignments. The program is run by designating specific sequences and their amino acid or nucleotide coordinates for regions of sequence comparison and by designating the program parameters. Using PILEUP, a reference sequence is compared to other test sequences to determine the percent sequence identity relationship using the following parameters: default gap weight (3.00), default gap length weight (0.10), and weighted end gaps. PILEUP can be obtained from the GCG sequence analysis software package, e.g., version 7.0 (Devereaux et al.,  Nuc. Acids Res.  12:387-395 (1984). 
     Another example of algorithm that is suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al.,  Nuc. Acids Res.  25:3389-3402 (1977) and Altschul et al.,  J. Mol. Biol.  215:403-410 (1990), respectively. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information (website at www.ncbi.nlm.nih.gov/). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always &gt;0) and N (penalty score for mismatching residues; always &lt;0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) or 10, M=5, N=−4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength of 3, and expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff &amp; Henikoff,  Proc. Natl. Acad. Sci. USA  89:10915 (1989)) alignments (B) of 50, expectation (E) of 10, M=5, N=−4, and a comparison of both strands. 
     The BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin &amp; Altschul,  Proc. Nat&#39;l. Acad. Sci. USA  90:5873-5787 (1993)). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01, and most preferably less than about 0.001. 
     Polynucleotide Identification and Characterisation 
     Polynucleotides encoding the  Clostridium difficile  CDTa, CDTb, Toxin A and Toxin B proteins of the invention may be identified, prepared and/or manipulated using any of a variety of well established techniques. For example, a polynucleotide may be identified, as described in more detail below, by screening a microarray of cDNAs. Such screens may be performed, for example, using a Synteni microarray (Palo Alto, Calif.) according to the manufacturer&#39;s instructions (and essentially as described by Schena et al.,  Proc. Natl. Acad. Sci. USA  93:10614-10619 (1996) and Heller et al.,  Proc. Natl. Acad. Sci. USA  94:2150-2155 (1997)). Alternatively, polynucleotides may be amplified from cDNA prepared from cells expressing the proteins described herein, such as  M. tuberculosis  cells. Such polynucleotides may be amplified via polymerase chain reaction (FOR). For this approach, sequence-specific primers may be designed based on the sequences provided herein, and may be purchased or synthesised. 
     An amplified portion of a polynucleotide may be used to isolate a full length gene from a suitable library (e.g., a  M. tuberculosis  cDNA library) using well known techniques. Within such techniques, a library (cDNA or genomic) is screened using one or more polynucleotide probes or primers suitable for amplification. Preferably, a library is size-selected to include larger molecules. Random primed libraries may also be preferred for identifying 5′ and upstream regions of genes. Genomic libraries are preferred for obtaining introns and extending 5′ sequences. 
     For hybridisation techniques, a partial sequence may be labeled (e.g., by nick-translation or end-labeling with  32 P) using well known techniques. A bacterial or bacteriophage library is then generally screened by hybridising filters containing denatured bacterial colonies (or lawns containing phage plaques) with the labeled probe (see Sambrook et al.,  Molecular Cloning: A Laboratory Manual  (2000)). Hybridising colonies or plaques are selected and expanded, and the DNA is isolated for further analysis. cDNA clones may be analyzed to determine the amount of additional sequence by, for example, PCR using a primer from the partial sequence and a primer from the vector. Restriction maps and partial sequences may be generated to identify one or more overlapping clones. The complete sequence may then be determined using standard techniques, which may involve generating a series of deletion clones. The resulting overlapping sequences can then be assembled into a single contiguous sequence. A full length cDNA molecule can be generated by ligating suitable fragments, using well known techniques. 
     Alternatively, there are numerous amplification techniques for obtaining a full length coding sequence from a partial cDNA sequence. Within such techniques, amplification is generally performed via PCR. Any of a variety of commercially available kits may be used to perform the amplification step. Primers may be designed using, for example, software well known in the art. Primers are preferably 22-30 nucleotides in length, have a GC content of at least 50% and anneal to the target sequence at temperatures of about 68° C. to 72° C. The amplified region may be sequenced as described above, and overlapping sequences assembled into a contiguous sequence. 
     One such amplification technique is inverse PCR (see Triglia et al.,  Nucl. Acids Res.  16:8186 (1988)), which uses restriction enzymes to generate a fragment in the known region of the gene. The fragment is then circularised by intramolecular ligation and used as a template for PCR with divergent primers derived from the known region. Within an alternative approach, sequences adjacent to a partial sequence may be retrieved by amplification with a primer to a linker sequence and a primer specific to a known region. The amplified sequences are typically subjected to a second round of amplification with the same linker primer and a second primer specific to the known region. A variation on this procedure, which employs two primers that initiate extension in opposite directions from the known sequence, is described in WO 96/38591. Another such technique is known as “rapid amplification of cDNA ends” or RACE. This technique involves the use of an internal primer and an external primer, which hybridises to a polyA region or vector sequence, to identify sequences that are 5′ and 3′ of a known sequence. Additional techniques include capture PCR (Lagerstrom et al.,  PCR Methods Apptic.  1:111-19 (1991)) and walking PCR (Parker et al.,  Nucl. Acids. Res.  19:3055-60 (1991)). Other methods employing amplification may also be employed to obtain a full length cDNA sequence. 
     In certain instances, it is possible to obtain a full length cDNA sequence by analysis of sequences provided in an expressed sequence tag (EST) database, such as that available from GenBank. Searches for overlapping ESTs may generally be performed using well known programs (e.g., NCBI BLAST searches), and such ESTs may be used to generate a contiguous full length sequence. Full length DNA sequences may also be obtained by analysis of genomic fragments. 
     Polynucleotide Expression in Host Cells 
     Polynucleotide sequences or fragments thereof which encode the  Clostridium difficile  CDTa, CDTb, Toxin A and Toxin B proteins, or fusion proteins or functional equivalents thereof, may be used in recombinant DNA molecules to direct expression of a polypeptide in appropriate host cells. Due to the inherent degeneracy of the genetic code, other DNA sequences that encode substantially the same or a functionally equivalent amino acid sequence may be produced and these sequences may be used to clone and express a given polypeptide. 
     As will be understood by those of skill in the art, it may be advantageous in some instances to produce polypeptide-encoding nucleotide sequences possessing non-naturally occurring codons. For example, codons preferred by a particular prokaryotic or eukaryotic host can be selected to increase the rate of protein expression or to produce a recombinant RNA transcript having desirable properties, such as a half-life which is longer than that of a transcript generated from the naturally occurring sequence. 
     Moreover, the polynucleotide sequences can be engineered using methods generally known in the art in order to alter polypeptide encoding sequences for a variety of reasons, including but not limited to, alterations which modify the cloning, processing, and/or expression of the gene product. For example, DNA shuffling by random fragmentation and PCR reassembly of gene fragments and synthetic oligonucleotides may be used to engineer the nucleotide sequences. In addition, site-directed mutagenesis may be used to insert new restriction sites, alter glycosylation patterns, change codon preference, produce splice variants, or introduce mutations, and so forth. 
     Natural, modified, or recombinant nucleic acid sequences may be ligated to a heterologous sequence to encode a fusion protein. For example, to screen peptide libraries for inhibitors of polypeptide activity, it may be useful to encode a chimeric protein that can be recognised by a commercially available antibody. A fusion protein may also be engineered to contain a cleavage site located between the polypeptide-encoding sequence and the heterologous protein sequence, so that the polypeptide may be cleaved and purified away from the heterologous moiety. 
     Sequences encoding a desired polypeptide may be synthesised, in whole or in part, using chemical methods well known in the art (see Caruthers, M. H. et al.,  Nucl. Acids Res. Symp. Ser. pp.  215-223 (1980), Horn et al.,  Nucl. Acids Res. Symp. Ser.  pp. 225-232 (1980)). Alternatively, the protein itself may be produced using chemical methods to synthesize the amino acid sequence of a polypeptide, or a portion thereof. For example, peptide synthesis can be performed using various solid-phase techniques (Roberge et al., Science 269:202-204 (1995)) and automated synthesis may be achieved, for example, using the ABI 431A Peptide Synthesizer (Perkin Elmer, Palo Alto, Calif.). 
     A newly synthesised peptide may be substantially purified by preparative high performance liquid chromatography (e.g., Creighton,  Proteins, Structures and Molecular Principles  (1983)) or other comparable techniques available in the art. The composition of the synthetic peptides may be confirmed by amino acid analysis or sequencing (e.g., the Edman degradation procedure). Additionally, the amino acid sequence of a polypeptide, or any part thereof, may be altered during direct synthesis and/or combined using chemical methods with sequences from other proteins, or any part thereof. to produce a variant polypeptide. 
     In order to express a desired polypeptide, the nucleotide sequences encoding the polypeptide, or functional equivalents, may be inserted into an appropriate expression vector, i.e., a vector which contains the necessary elements for the transcription and translation of the inserted coding sequence. Methods which are well known to those skilled in the art may be used to construct expression vectors containing sequences encoding a polypeptide of interest and appropriate transcriptional and translational control elements. These methods include in vitro recombinant DNA techniques, synthetic techniques, and in vivo genetic recombination. Such techniques are described in Sambrook et al.,  Molecular Cloning, A Laboratory Manual  (2000), and Ausubel et al.,  Current Protocols in Molecular Biology  (updated annually). 
     A variety of expression vector/host systems may be utilised to contain and express polynucleotide sequences. These include, but are not limited to, microorganisms such as bacteria transformed with recombinant bacteriophage, plasmid, or cosmid DNA expression vectors; yeast transformed with yeast expression vectors; insect cell systems infected with virus expression vectors (e.g., baculovirus); plant cell systems transformed with virus expression vectors (e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or with bacterial expression vectors (e.g., Ti or pBR322 plasmids): or animal cell systems. 
     The “control elements” or “regulatory sequences” present in an expression vector are those non-translated regions of the vector-enhancers, promoters, 5′ and 3′ untranslated regions—which interact with host cellular proteins to carry out transcription and translation. Such elements may vary in their strength and specificity. Depending on the vector system and host utilised, any number of suitable transcription and translation elements, including constitutive and inducible promoters, may be used. For example, when cloning in bacterial systems, inducible promoters such as the hybrid lacZ promoter of the PBLUESCRIPT phagemid (Stratagene, La Jolla, Calif.) or PSPORTI plasmid (Gibco BRL, Gaithersburg, Md.) and the like may be used. In mammalian cell systems, promoters from mammalian genes or from mammalian viruses are generally preferred. If it is necessary to generate a cell line that contains multiple copies of the sequence encoding a polypeptide, vectors based on SV40 or EBV may be advantageously used with an appropriate selectable marker. 
     In bacterial systems, a number of expression vectors may be selected depending upon the use intended for the expressed polypeptide. For example, when large quantities are needed, for example for the induction of antibodies, vectors which direct high level expression of fusion proteins that are readily purified may be used. Such vectors include, but are not limited to, the multifunctional  E. coli  cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke &amp; Schuster,  J. Biol. Chem.  264:5503-5509 (1989)); and the like. pGEX Vectors (Promega, Madison, Wis.; GE Healthcare.) may also be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST). In general, such fusion proteins are soluble and can easily be purified from lysed cells by adsorption to glutathione-agarose beads followed by elution in the presence of free glutathione. Proteins made in such systems may be designed to include heparin, thrombin, or factor XA protease cleavage sites so that the cloned polypeptide of interest can be released from the GST moiety at will. 
     In the yeast,  Saccharomyces cerevisiae  or  Pichia  such as  Pichia  pastoris for example, a number of vectors containing constitutive or inducible promoters such as alpha factor, alcohol oxidase, and PGH may be used. Other vectors containing constitutive or inducible promoters include GAP, PGK, GAL and ADH. For reviews, see Ausubel et al. (supra) and Grant et al.,  Methods Enzymol.  153:516-544 (1987) and Romas et al.  Yeast  8 423-88 (1992). 
     In cases where plant expression vectors are used, the expression of sequences encoding polypeptides may be driven by any of a number of promoters. For example, viral promoters such as the 35S and 19S promoters of CaMV may be used alone or in combination with the omega leader sequence from TMV (Takamatsu,  EMBO J.  6:307-311 (1987)). Alternatively, plant promoters such as the small subunit of RUBISCO or heat shock promoters may be used (Coruzzi et al.,  EMBO J.  3:1671-1680 (1984); Broglie et al., Science 224:838-843 (1984); and Winter et al.,  Results Probl. Cell Differ.  17:85-105 (1991)). These constructs can be introduced into plant cells by direct DNA transformation or pathogen-mediated transfection. Such techniques are described in a number of generally available reviews (see, e.g., Hobbs in  McGraw Hill Yearbook of Science and Technology  pp. 191-196 (1992)). 
     An insect system may also be used to express a polypeptide of interest. For example, in one such system,  Autographa californica  nuclear polyhedrosis virus (AcNPV) is used as a vector to express foreign genes in  Spodoptera frugiperda  cells or in  Trichoplusia larvae.  The sequences encoding the polypeptide may be cloned into a non-essential region of the virus, such as the polyhedrin gene, and placed under control of the polyhedrin promoter. Successful insertion of the polypeptide-encoding sequence will render the polyhedrin gene inactive and produce recombinant virus lacking coat protein. The recombinant viruses may then be used to infect, for example,  S. frugiperda  cells or  Trichoplusia larvae  in which the polypeptide of interest may be expressed (Engelhard et al.,  Proc. Natl. Acad. Sci. U.S.A.  91:3224-3227 (1994)). 
     In mammalian host cells, a number of viral-based expression systems are generally available. For example, in cases where an adenovirus is used as an expression vector, sequences encoding a polypeptide of interest may be ligated into an adenovirus transcription/translation complex consisting of the late promoter and tripartite leader sequence. Insertion in a non-essential E1 or E3 region of the viral genome may be used to obtain a viable virus which is capable of expressing the polypeptide in infected host cells (Logan &amp; Shenk,  Proc. Natl. Acad. Sci. U.S.A.  81:3655-3659 (1984)). In addition, transcription enhancers, such as the Rous sarcoma virus (RSV) enhancer, may be used to increase expression in mammalian host cells. Methods and protocols for working with adenovirus vectors are reviewed in Wold,  Adenovirus Methods and Protocols,  1998. Additional references regarding use of adenovirus vectors can be found in  Adenovirus: A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References,  2004. 
     Specific initiation signals may also be used to achieve more efficient translation of sequences encoding a polypeptide of interest. Such signals include the ATG initiation codon and adjacent sequences. In cases where sequences encoding the polypeptide, its initiation codon, and upstream sequences are inserted into the appropriate expression vector, no additional transcriptional or translational control signals may be needed. However, in cases where only coding sequence, or a portion thereof, is inserted, exogenous translational control signals including the ATG initiation codon should be provided. Furthermore, the initiation codon should be in the correct reading frame to ensure translation of the entire insert. Exogenous translational elements and initiation codons may be of various origins, both natural and synthetic. The efficiency of expression may be enhanced by the inclusion of enhancers which are appropriate for the particular cell system which is used, such as those described in the literature (Scharf. et al.  Results Probl. Cell Differ.  20:125-162 (1994)). 
     In addition, a host cell strain may be chosen for its ability to modulate the expression of the inserted sequences or to process the expressed protein in the desired fashion. Such modifications of the polypeptide include, but are not limited to, acetylation, carboxylation, glycosylation, phosphorylation, lipidation, and acylation. Post-translational processing which cleaves a “prepro” form of the protein may also be used to facilitate correct insertion, folding and/or function. Different host cells such as CHO, HeLa, MDCK, HEK293, and Wl38, which have specific cellular machinery and characteristic mechanisms for such post-translational activities, may be chosen to ensure the correct modification and processing of the foreign protein. 
     For long-term, high-yield production of recombinant proteins, stable expression is generally preferred. For example, cell lines which stably express a polynucleotide of interest may be transformed using expression vectors which may contain viral origins of replication and/or endogenous expression elements and a selectable marker gene on the same or on a separate vector. Following the introduction of the vector, cells may be allowed to grow for 1-2 days in an enriched media before they are switched to selective media. The purpose of the selectable marker is to confer resistance to selection, and its presence allows growth and recovery of cells which successfully express the introduced sequences. Resistant clones of stably transformed cells may be proliferated using tissue culture techniques appropriate to the cell type. 
     Any number of selection systems may be used to recover transformed cell lines. These include, but are not limited to, the herpes simplex virus thymidine kinase (Wigler et al.,  Cell  11:223-32 (1977)) and adenine phosphoribosyltransferase (Lowy et al.,  Cell  22:817-23 (1990)) genes which can be employed in tk.sup.- or aprt.sup.-cells, respectively. Also, antimetabolite, antibiotic or herbicide resistance can be used as the basis for selection; for example, dhfr which confers resistance to methotrexate (Wigler et al.,  Proc. Natl. Acad. Sci. U.S.A.  77:3567-70 (1980)); npt, which confers resistance to the aminoglycosides, neomycin and G-418 (Colbere-Garapin et al.,  J. Mol. Biol.  150:1-14 (1981)); and als or pat, which confer resistance to chlorsulfuron and phosphinotricin acetyltransferase, respectively (Murry, supra). Additional selectable genes have been described, for example, trpB, which allows cells to utilise indole in place of tryptophan, or hisD, which allows cells to utilise histinol in place of histidine (Hartman &amp; Mulligan,  Proc. Natl. Acad. Sci. U.S.A.  85:8047-51 (1988)). Recently, the use of visible markers has gained popularity with such markers as anthocyanins, β-glucuronidase and its substrate GUS, and luciferase and its substrate luciferin, being widely used not only to identify transformants, but also to quantify the amount of transient or stable protein expression attributable to a specific vector system (Rhodes et al.,  Methods Mol. Biol.  55:121-131 (1995)). 
     Although the presence/absence of marker gene expression suggests that the gene of interest is also present, its presence and expression may need to be confirmed. For example, if the sequence encoding a polypeptide is inserted within a marker gene sequence, recombinant cells containing sequences can be identified by the absence of marker gene function. Alternatively, a marker gene can be placed in tandem with a polypeptide-encoding sequence under the control of a single promoter. Expression of the marker gene in response to induction or selection usually indicates expression of the tandem gene as well. 
     Alternatively, host cells which contain and express a desired polynucleotide sequence may be identified by a variety of procedures known to those of skill in the art. These procedures include, but are not limited to. DNA-DNA or DNA-RNA hybridisations and protein bioassay or immunoassay techniques which include membrane, solution, or chip based technologies for the detection and/or quantification of nucleic acid or protein. 
     A variety of protocols for detecting and measuring the expression of polynucleotide-encoded products, using either polyclonal or monoclonal antibodies specific for the product are known in the art. Examples include enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), and fluorescence activated cell sorting (FACS). A two-site, monoclonal-based immunoassay utilising monoclonal antibodies reactive to two non-interfering epitopes on a given polypeptide may be preferred for some applications, but a competitive binding assay may also be employed. These and other assays are described, among other places, in Hampton et al.,  Serological Methods, a Laboratory Manual  (1990) and Maddox et al.,  J. Exp. Med.  158:1211-1216 (1983). 
     A wide variety of labels and conjugation techniques are known by those skilled in the art and may be used in various nucleic acid and amino acid assays. Means for producing labelled hybridisation or PCR probes for detecting sequences related to polynucleotides include oligolabeling, nick translation, end-labelling or PCR amplification using a labelled nucleotide. Alternatively, the sequences, or any portions thereof may be cloned into a vector for the production of an mRNA probe. Such vectors are known in the art, are commercially available, and may be used to synthesize RNA probes in vitro by addition of an appropriate RNA polymerase such as T7, T3, or SP6 and labeled nucleotides. These procedures may be conducted using a variety of commercially available kits. Suitable reporter molecules or labels, which may be used include radionuclides, enzymes, fluorescent, chemiluminescent, or chromogenic agents as well as substrates, cofactors, inhibitors, magnetic particles, and the like. 
     Host cells transformed with a polynucleotide sequence of interest may be cultured under conditions suitable for the expression and recovery of the protein from cell culture. The protein produced by a recombinant cell may be secreted or contained intracellularly depending on the sequence and/or the vector used. As will be understood by those of skill in the art, expression vectors containing polynucleotides may be designed to contain signal sequences which direct secretion of the encoded polypeptide through a prokaryotic or eukaryotic cell membrane. Other recombinant constructions may be used to join sequences encoding a polypeptide of interest to nucleotide sequence encoding a polypeptide domain which will facilitate purification of soluble proteins. Such purification facilitating domains include, but are not limited to, metal chelating peptides such as histidine-tryptophan modules that allow purification on immobilized metals, protein A domains that allow purification on immobilised immunoglobulin, and the domain utilized in the FLAGS extension/affinity purification system (Immunex Corp., Seattle, Wash.). The inclusion of cleavable linker sequences such as those specific for Factor XA or enterokinase (Invitrogen. San Diego, Calif.) between the purification domain and the encoded polypeptide may be used to facilitate purification. One such expression vector provides for expression of a fusion protein containing a polypeptide of interest and a nucleic acid encoding 6 histidine residues preceding a thioredoxin or an enterokinase cleavage site. The histidine residues facilitate purification on IMIAC (immobilised metal ion affinity chromatography) as described in Porath et al.,  Prot. Exp. Purif.  3:263-281 (1992) while the enterokinase cleavage site provides a means for purifying the desired polypeptide from the fusion protein. A discussion of vectors which contain fusion proteins is provided in Kroll et al.,  DNA Cell Biol.  12:441-453 (1993)). 
     Polypeptide Compositions 
     Generally, a polypeptide of use in the invention (for example the  Clostridium difficile  CDTa, CDTb, Toxin A and Toxin B proteins) will be an isolated polypeptide (i.e. separated from those components with which it may usually be found in nature). 
     For example, a naturally-occurring protein is isolated if it is separated from some or all of the coexisting materials in the natural system. Preferably, such polypeptides are at least about 90% pure, more preferably at least about 95% pure and most preferably at least about 99% pure. A polynucleotide is considered to be isolated if. for example, it is cloned into a vector that is not a part of the natural environment. 
     Polypeptides may be prepared using any of a variety of well known techniques. Recombinant polypeptides encoded by DNA sequences as described above may be readily prepared from the DNA sequences using any of a variety of expression vectors known to those of ordinary skill in the art. Expression may be achieved in any appropriate host cell that has been transformed or transfected with an expression vector containing a DNA molecule that encodes a recombinant polypeptide. Suitable host cells include prokaryotes, yeast, and higher eukaryotic cells, such as mammalian cells and plant cells. Preferably, the host cells employed are  E. coli,  yeast or a mammalian cell line such as COS or CHO. Supernatants from suitable host/vector systems which secrete recombinant protein or polypeptide into culture media may be first concentrated using a commercially available filter. Following concentration, the concentrate may be applied to a suitable purification matrix such as an affinity matrix or an ion exchange resin. Finally, one or more reverse phase HPLC steps can be employed to further purify a recombinant polypeptide. 
     Polypeptides for use in the invention, immunogenic fragments thereof, and other variants having less than about 100 amino acids, and generally less than about 50 amino acids, may also be generated by synthetic means, using techniques well known to those of ordinary skill in the art. For example, such polypeptides may be synthesised using any of the commercially available solid-phase techniques, such as the Merrifield solid-phase synthesis method, where amino acids are sequentially added to a growing amino acid chain. See Merrifield,  J. Am. Chem. Soc.  85:2149-2146 (1963). Equipment for automated synthesis of polypeptides is commercially available from suppliers such as Perkin Elmer/Applied BioSystems Division (Foster City, Calif.), and may be operated according to the manufacturer&#39;s instructions. 
     Within certain specific embodiments, a polypeptide may be a fusion protein that comprises multiple polypeptides as described herein, or that comprises at least one polypeptide as described herein and an unrelated sequence, examples of such proteins include tetanus, tuberculosis and hepatitis proteins (see, e.g., Stoute et al.,  New Engl. J. Med.  336:86-91 (1997)). A fusion partner may, for example, assist in providing T helper epitopes (an immunological fusion partner), preferably T helper epitopes recognised by humans, or may assist in expressing the protein (an expression enhancer) at higher yields than the native recombinant protein. Certain preferred fusion partners are both immunological and expression enhancing fusion partners. Other fusion partners may be selected so as to increase the solubility of the protein or to enable the protein to be targeted to desired intracellular compartments. Still further fusion partners include affinity tags, which facilitate purification of the protein. 
     Fusion proteins may generally be prepared using standard techniques, including chemical conjugation. Preferably, a fusion protein is expressed as a recombinant protein, allowing the production of increased levels, relative to a non-fused protein, in an expression system. Briefly, DNA sequences encoding the polypeptide components may be assembled separately, and ligated into an appropriate expression vector. The 3′ end of the DNA sequence encoding one polypeptide component is ligated, with or without a peptide linker, to the 5′ end of a DNA sequence encoding the second polypeptide component so that the reading frames of the sequences are in phase. This permits translation into a single fusion protein that retains the biological activity of both component polypeptides. 
     A peptide linker sequence may be employed to separate the first and second polypeptide components by a distance sufficient to ensure that each polypeptide folds into its secondary and tertiary structures. Such a peptide linker sequence is incorporated into the fusion protein using standard techniques well known in the art. Suitable peptide linker sequences may be chosen based on the following factors: (1) their ability to adopt a flexible extended conformation; (2) their inability to adopt a secondary structure that could interact with functional epitopes on the first and second polypeptides; and (3) the lack of hydrophobic or charged residues that might react with the polypeptide functional epitopes. Preferred peptide linker sequences contain Gly, Asn and Ser residues. Other near neutral amino acids, such as Thr and Ala may also be used in the linker sequence. Amino acid sequences which may be usefully employed as linkers include those disclosed in Maratea et al.,  Gene  40:39-46 (1985); Murphy et al.,  Proc. Natl. Acad. Sci. USA  83:8258-8262 (1986); U.S. Pat. No. 4,935,233 and U.S. Pat. No. 4,751,180. The linker sequence may generally be from 1 to about 50 amino acids in length. Linker sequences are not required when the first and second polypeptides have non-essential N-terminal amino acid regions that can be used to separate the functional domains and prevent steric interference. 
     Adjuvants 
     In a further embodiment of any of the aspects of the invention, the immunogenic composition further comprises an adjuvant. In one embodiment the adjuvant comprises aluminium hydroxide or aluminium phosphate. Alternatively the immunogenic composition of the invention may comprise an aluminium-free adjuvant, the immunogenic composition is formulated with an adjuvant that is free of aluminum or aluminum salts, that is, an aluminum-free adjuvant or adjuvant system. 
     In certain embodiments, the immunogenic composition is formulated with an adjuvant comprising an immunologically active saponin fraction presented in the form of a liposome. The adjuvant may further comprise a lipopolysaccharide. The adjuvant may include QS21. For example, in one embodiment, the adjuvant contains QS21 in a liposomal formulation. In one embodiment, the adjuvant system includes 3D-MPL and QS21. For example, in one embodiment, the adjuvant contains 3D-MPL and QS21 in a liposomal formulation. Optionally, the adjuvant system also contains cholesterol. In one specific embodiment, the adjuvant includes QS21 and cholesterol. Optionally, the adjuvant system contains 1, 2-Dioleoyl-sn-Glycero-3-phosphocholine (DOPC). For example, in one specific adjuvant system contains cholesterol, DOPC, 3D-MPL and QS21. 
     In one specific example, the immunogenic composition includes an adjuvant formulated in a dose that includes: from about 0.1 to about 0.5 mg cholesterol; from about 0.25 to about 2 mg DOPC; from about 10 μg to about 100 μg 3D-MPL; and from about 10 μg to about 100 pg QS21. In another specific example, the immunogenic composition includes an adjuvant formulated in a dose that includes: from about 0.1 to about 0.5 mg cholesterol; from about 0.25 to about 2 mg DOPC; from about 10 μg to about 70 μg 3D-MPL; and from about 10 μg to about 70 μg QS21. In one specific formulation, the adjuvant is formulated in a single dose that contains: about 0.25 mg cholesterol; about 1.0 mg DOPC; about 50 μg 3D-MPL; and about 50 μg QS21. In other embodiments, the immunogenic composition is formulated with a fractional dose (that is a dose, which is a fraction of the preceding single dose formulations, such as one half of the preceding quantity of components (cholesterol, DOPC, 3D-MPL and QS21), ¼ of the preceding quantity of components, or another fractional dose (e.g., ⅓, ⅙, etc.) of the preceding quantity of components. 
     In one embodiment, the immunogenic compositions according to the invention include an adjuvant containing combinations of lipopolysaccharide and Quillaja saponins that have been disclosed previously, for example in EP0671948. This patent demonstrated a strong synergy when a lipopolysaccharide (3D-MPL) was combined with a Quillaja saponin (QS21). 
     The adjuvant may further comprise immunostimulatory oligonucleotides (for example, CpG) or a carrier. 
     A particularly suitable saponin for use in the present invention is Quil A and its derivatives. Quil A is a saponin preparation isolated from the South American tree  Quillaja Saponaria Molina  and was first described by Dalsgaard et al. in 1974 (“Saponin adjuvants”, Archiv. fur die gesamte Virusforschung, Vol. 44, Springer Verlag, Berlin, p243-254) to have adjuvant activity. Purified fragments of Quil A have been isolated by HPLC which retain adjuvant activity without the toxicity associated with Quil A (EP 0 362 278), for example QS7 and QS21 (also known as QA7 and QA21). QS21 is a natural saponin derived from the bark of Quillaja saponaria Molina, which induces CD8+ cytotoxic T cells (CTLs), Th1 cells and a predominant IgG2a antibody response and is a preferred saponin in the context of the present invention. 
     When the adjuvant comprises an immunologically active saponin fraction presented in the form of a liposome, the adjuvant may further comprise a sterol. Suitably the sterol is provided at a ratio of saponin:sterol of from 1:1 to 1:100 w/w, such as from 1:1 to 1:10w/w; or 1:1 to 1:5 w/w. 
     In a specific embodiment, QS21 is provided in its less reactogenic composition where it is quenched with an exogenous sterol, such as cholesterol for example. Several particular forms of less reactogenic compositions wherein QS21 is quenched with an exogenous cholesterol exist. In a specific embodiment, the saponin/sterol is in the form of a liposome structure (WO 96/33739, Example 1). In this embodiment the liposomes suitably contain a neutral lipid, for example phosphatidylcholine, which is suitably non-crystalline at room temperature, for example eggyolk phosphatidylcholine, dioleoyl phosphatidylcholine (DOPC) or dilauryl phosphatidylcholine. The liposomes may also contain a charged lipid which increases the stability of the lipsome-QS21 structure for liposomes composed of saturated lipids. In these cases the amount of charged lipid is suitably 1-20% w/w, preferably 5-10%. The ratio of sterol to phospholipid is 1-50% (mol/mol), suitably 20-25%. 
     Suitable sterols include f3-sitosterol, stigmasterol, ergosterol, ergocalciferol and cholesterol. In one particular embodiment, the adjuvant composition comprises cholesterol as sterol. These sterols are well known in the art, for example cholesterol is disclosed in the Merck Index, 11th Edn., page 341, as a naturally occurring sterol found in animal fat. 
     Where the active saponin fraction is QS21, the ratio of QS21 : sterol will typically be in the order of 1:100 to 1:1 (w/w), suitably between 1:10 to 1:1 (w/w), and preferably 1:5 to 1:1 (w/w). Suitably excess sterol is present, the ratio of QS21:sterol being at least 1:2 (w/w). In one embodiment, the ratio of QS21:sterol is 1:5 (w/w). The sterol is suitably cholesterol. 
     In one embodiment, the invention provides a dose of an immunogenic composition comprising immunologically active saponin, preferably QS21, at a level of about 1-about 70 μg per dose, for example at an amount of about 50 μg. 
     In one embodiment, the invention provides a dose of an immunogenic composition comprising immunologically active saponin, preferably QS21, at a level of 60 μg or less, for example between 1 and 60 μg. In one embodiment, the dose of the immunogenic composition comprises QS21 at a level of approximately around 50 μg, for example between 45 and 55 μg. suitably between 46-54 μg or between 47 and 53 μg or between 48 and 52 μg or between 49 and 51 μg, or 50 μg. 
     In another embodiment the dose of the immunogenic composition comprises QS21 at a level of around 25 μg, for example between 20-30 μg, suitably between 21-29 μg or between 22 and 28 μg or between 23 and 27 μg or between 24 and 26 μg, or 25 μg. 
     In another embodiment, the dose of the immunogenic composition comprises QS21 at a level of around 10 μg per, for example between 5 and 15 μg, suitably between 6 and 14 μg, for example between 7 and 13 μg or between 8 and 12 μg or between 9 and 11 μg, or 10 μg. 
     Specifically, a 0.5 ml vaccine dose volume contains 25 μg or 50 μg of QS21 per dose. 
     Specifically, a 0.5 ml vaccine dose volume contains 50 μg of QS21 per dose. 
     In compositions comprising a lipopolysaccharide, the lipopolysaccharide may be present at an amount of about 1-about 70 μg per dose, for example at an amount of about 50 μg. 
     The lipopolysaccharide may be a non-toxic derivative of lipid A, particularly monophosphoryl lipid A or more particularly 3-Deacylated monophoshoryl lipid A (3D-MPL). 
     3D-MPL is sold under the name MPL by GlaxoSmithKline Biologicals S. A. and is referred throughout the document as MPL or 3D-MPL. See, for example, U.S. Pat. Nos. 4,436,727; 4,877,611; 4,866,034 and 4.912,094. 3D-MPL primarily promotes CD4+ T cell responses with an IFN-γ (Th1) phenotype. 3D-MPL can be produced according to the methods disclosed in GB 2 220 211 A. Chemically it is a mixture of 3-deacylated monophosphoryl lipid A with 3, 4. 5 or 6 acylated chains. Preferably in the compositions of the present invention small particle 3D-MPL is used. Small particle 3D-MPL has a particle size such that it may be sterile-filtered through a 0.22 μm filter. Such preparations are described in WO 94/21292. 
     The invention therefore provides a dose of an immunogenic composition comprising lipopolysaccharide, preferably 3D-MPL, at a level of 75 μg or less, for example between 1 and 60 μg. 
     In one embodiment, the dose of the immunogenic composition comprises 3D-MPL at a level of around 50 μg, for example between 45-55 μg, suitably between 46-54 μg or between 47 and 53 μg or between 48 and 52 μg or between 49 and 51 μg, or 50 μg. 
     In one embodiment, the dose of the immunogenic composition comprises 3D-MPL at a level of around 25 μg, for example between 20-30 μg, suitably between 21-29 μg or between 22 and 28 μg or between 23 and 27 μg or between 24 and 26 μg, or 25 μg. 
     In another embodiment, the dose of the immunogenic composition comprises 3D-MPL at a level of around 10 μg, for example between 5 and 15 μg, suitably between 6 and 14 μg, for example between 7 and 13 μg or between 8 and 12 μg or between 9 and 11 μg, or 10 μg. 
     In one embodiment, the volume of the dose is 0.5 ml. In a further embodiment, the immunogenic composition is in a volume suitable for a dose which volume is higher than 0.5 ml, for example 0.6, 0.7, 0.8, 0.9 or 1 ml. In a further embodiment, the human dose is between 1 ml and 1.5 ml. 
     Specifically, a 0.5 ml vaccine dose volume contains 25 μg or 50 μg of 3D-MPL per dose. Specifically, a 0.5 ml vaccine dose volume contains 50 μg of 3D-MPL per dose. 
     The dose of the immunogenic composition according to any aspect of the invention suitably refers to human dose. By the term “human dose” is meant a dose which is in a volume suitable for human use. Generally this is between 0.3 and 1.5 ml. In one embodiment, a human dose is 0.5 ml. In a further embodiment, a human dose is higher than 0.5 ml, for example 0.6, 0.7, 0.8, 0.9 or 1 ml. In a further embodiment, a human dose is between 1 ml and 1.5 ml. 
     Suitable compositions of the invention are those wherein liposomes are initially prepared without MPL (as described in WO 96/33739), and MPL is then added, suitably as small particles of below 100 nm particles or particles that are susceptible to sterile filtration through a 0.22 μm membrane. The MPL is therefore not contained within the vesicle membrane (known as MPL out). Compositions where the MPL is contained within the vesicle membrane (known as MPL in) also form an aspect of the invention. The polypeptide comprising a  C. difficile  toxin A fragment and/or a  C. difficile  toxin B fragment can be contained within the vesicle membrane or contained outside the vesicle membrane. 
     In a specific embodiment, QS21 and 3D-MPL are present in the same final concentration per dose of the immunogenic composition i.e. the ratio of QS21:3D-MPL is 1:1. In one aspect of this embodiment, a dose of immunogenic composition comprises a final level of 25 μg of 3D-MPL and 25 μg of QS21 or 50 μg of 3D-MPL and 50 μg of QS21. 
     In one embodiment, the adjuvant includes an oil-in-water emulsion. In one embodiment the adjuvant comprises an oil in water emulsion, wherein the oil in water emulsion comprises a metabolisable oil, a tocol and an emulsifier. For example, the oil-in-water emulsion can include an oil phase that incorporates a metabolisable oil, and an additional oil phase component, such as a tocol. The oil-in-water emulsion may also contain an aqueous component, such as a buffered saline solution (e.g., phosphate buffered saline). In addition, the oil-in-water emulsion typically contains an emulsifier. In one embodiment, the metabolizable oil is squalene. In one embodiment, the tocol is alpha-tocopherol. In one embodiment, the emulsifier is a nonionic surfactant emulsifier (such as polyoxyethethylene sorbitan monooleate, Polysorbate® 80 , TWEEN80™). In exemplary embodiments, the oil-in-water emulsion contains squalene and alpha tocopherol in a ratio which is equal or less than 1 (w/w). 
     The metabolisable oil in the oil-in-water emulsion may be present in an amount of 0.5-10 mg. The tocol in the oil-in-water emulsion may be present in an amount of 0.5-11 mg. The emulsifying agent may be present in an amount of 0.4-4 mg. 
     In order for any oil in water composition to be suitable for human administration, the oil phase of the emulsion system has to comprise a metabolisable oil. The meaning of the term metabolisable oil is well known in the art. Metabolisable can be defined as ‘being capable of being transformed by metabolism’ (Dorland&#39;s Illustrated Medical Dictionary, W. B. Sanders Company, 25th edition (1974)). The oil may be any vegetable oil, fish oil, animal oil or synthetic oil, which is not toxic to the recipient and is capable of being transformed by metabolism. Nuts, seeds, and grains are common sources of vegetable oils. Synthetic oils are also part of this invention and can include commercially available oils such as NEOBEE® (caprylic/capric triglycerides made using glycerol from vegetable oil sources and medium-chain fatty acids (MCTs) from coconut or palm kernel oils) and others. A particularly suitable metabolisable oil is squalene. Squalene (2,6,10,15,19,23-Hexamethyl-2,6,10,14,18,22-tetracosahexaene) is an unsaturated oil which is found in large quantities in shark-liver oil, and in lower quantities in olive oil, wheat germ oil, rice bran oil, and yeast, and is a particularly preferred oil for use in this invention. Squalene is a metabolisable oil by virtue of the fact that it is an intermediate in the biosynthesis of cholesterol (Merck index, 10th Edition, entry no.8619). 
     Suitably the metabolisable oil is present in the adjuvant composition in an amount of 0.5-10 mg, preferably 1-10, 2-10, 3-9, 4-8, 5-7. or 5-6 mg (e.g. 2-3, 5-6, or 9-10 mg), specifically about 5.35 mg or about 2.14 mg per dose. 
     Tocols are well known in the art and are described in EP0382271. Suitably the tocol is alpha-tocopherol or a derivative thereof such as alpha-tocopherol succinate (also known as vitamin E succinate). Said tocol is suitably present in in an amount of 0.5-11 mg, preferably 1-11, 2-10, 3-9, 4-8, 5-7, 5-6 mg (e.g. 10-11, 5-6, 2.5-3.5 or 1-3 mg). In a specific embodiment the tocol is present in an amount of about 5.94 mg or about 2.38 mg per dose. 
     The oil in water emulsion further comprises an emulsifying agent. The emulsifying agent may suitably be polyoxyethylene sorbitan monooleate. In a particular embodiment the emulsifying agent may be Polysorbate® 80 (Polyoxyethylene (20) sorbitan monooleate) or Tween® 80. 
     Said emulsifying agent is suitably present in the adjuvant composition in an amount of 0.1-5, 0.2-5, 0.3-4, 0.4-3 or 2-3 mg (e.g. 0.4-1.2, 2-3 or 4-5 mg) emulsifying agent. In a specific embodiment the emulsifying agent is present in an amount of about 0.97 mg or about 2.425 mg. 
     In one embodiment, the amounts of specific components present in the composition are the amounts present in a 0.5 ml human dose. In a further embodiment, the immunogenic composition is in a volume suitable for a human dose which volume is higher than 0.5 ml, for example 0.6, 0.7, 0.8, 0.9 or 1 ml. In a further embodiment, the human dose is between 1 ml and 1.5 ml. 
     Where the adjuvant is in a liquid form and is to be combined with a liquid form of a polypeptide composition, the adjuvant composition in a human dose will be a fraction of the intended final volume of the human dose, for example approximately half of the intended final volume of the human dose, for example a 350 μl volume for an intended human dose of 0.7 ml, or a 250 μl volume for an intended human dose of 0.5 ml. The adjuvant composition is diluted when combined with the polypeptide antigen composition to provide the final human dose of vaccine. The final volume of such dose will of course vary dependent on the initial volume of the adjuvant composition and the volume of polypeptide antigen composition added to the adjuvant composition. In an alternative embodiment, a liquid adjuvant is used to reconstitute a lyophilised polypeptide composition. In this embodiment, the human dose of the adjuvant composition is approximately equal to the final volume of the human dose. The liquid adjuvant composition is added to the vial containing the lyophilised polypeptide composition. The final human dose can vary between 0.5 and 1.5 ml. 
     The method of producing oil-in-water emulsions is well known to the person skilled in the art. Commonly, the method comprises mixing the tocol-containing oil phase with a surfactant such as a PBS/polyoxyethylene sorbitan monooleate solution, followed by homogenisation using a homogenizer. It would be clear to a man skilled in the art that a method comprising passing the mixture twice through a syringe needle would be suitable for homogenising small volumes of liquid. Equally, the emulsification process in microfluidiser (M110S Microfluidics machine, maximum of 50 passes, for a period of 2 minutes at maximum pressure input of 6 bar (output pressure of about 850 bar)) could be adapted by the man skilled in the art to produce smaller or larger volumes of emulsion. The adaptation could be achieved by routine experimentation comprising the measurement of the resultant emulsion until a preparation was achieved with oil droplets of the required diameter. 
     In an oil in water emulsion, the oil and emulsifier should be in an aqueous carrier. The aqueous carrier may be, for example, phosphate buffered saline. 
     Preferably the oil-in-water emulsion systems of the present invention have a small oil droplet size in the sub-micron range. Suitably the droplet sizes will be in the range 120 to 750 nm, more preferably sizes from 120 to 600 nm in diameter. Most preferably the oil-in water emulsion contains oil droplets of which at least 70% by intensity are less than 500 nm in diameter, more preferably at least 80% by intensity are less than 300 nm in diameter, more preferably at least 90% by intensity are in the range of 120 to 200 nm in diameter. 
     In one embodiment, the immunogenic composition is not 3 μg or 10 μg of any of SEQ ID Nos. 1 to 7 combined with an adjuvant comprising an oil in water emulsion having 0.125 mL SB62 emulsion (Total volume), 5.35 mg squalene, 5.94 mg DL-α-tocopherol and 2.425 mg polysorbate 80 per 0.5 ml dose. In one embodiment, the immunogenic composition is not 3 μg or 10 μg of any of SEQ ID Nos. 1 to 7 combined with an adjuvant comprising an oil in water emulsion 5.35 mg squalene, 5.94 mg DL-a-tocopherol and 2.425 mg polysorbate 80 per 0.5 ml dose. In one embodiment, the immunogenic composition does not contain an adjuvant comprising a oil in water emulsion having squalene, DL-α-tocopherol and polysorbate 80. 
     Immunogenic Compositions and Vaccines of the Invention 
     In one embodiment the immunogenic composition has a volume of 0.5 to 1.5 ml. 
     In one embodiment the immunogenic composition further comprises additional antigens. In one embodiment the additional antigens are antigens derived from a bacterium selected from the group consisting of  S. pneumoniae, H. influenzae, N. meningitidis, E. coli, M. catarrhalis, Clostridium tetani  (tetanus),  Corynebacterium diphtheria  (diphtheria),  Bordetella pertussis  (pertussis),  S. epidermidis, enterococci, S. aureus,  and  Pseudomonas aeruginosa.  In a further embodiment the immunogenic composition of the invention may comprise further antigens from  C. difficile  for example the S-layer proteins (WO01/73030). Optionally the immunogenic composition further comprises a saccharide from  C. difficile.    
     There is further provided a vaccine comprising an immunogenic composition of the invention and a pharmaceutically acceptable excipient. 
     The vaccine preparations containing immunogenic compositions of the present invention may be used to protect a mammal susceptible to  C. difficile  infection or treat a mammal with a  C. difficile  infection, by means of administering said vaccine via systemic or mucosal route. These administrations may include injection via the intramuscular, intraperitoneal, intradermal or subcutaneous routes; or via mucosal administration to the oral/alimentary, respiratory, genitourinary tracts. Although the vaccine of the invention may be administered as a single dose, components thereof may also be co-administered together at the same time or at different times (for instance pneumococcal saccharide conjugates could be administered separately, at the same time or 1-2 weeks after the administration of the any bacterial protein component of the vaccine for coordination of the immune responses with respect to each other). In addition to a single route of administration, 2 different routes of administration may be used. For example, saccharides or saccharide conjugates may be administered intramuscularly (IM) or intradermally (ID) and bacterial proteins may be administered intranasally (IN) or intradermally (ID). In addition, the vaccines of the invention may be administered IM for priming doses and IN for booster doses. 
     The content of toxins in the vaccine will typically be in the range 1-250 μg, preferably 5-50 μg, most typically in the range 5-25 μg. Following an initial vaccination, subjects may receive one or several booster immunizations adequately spaced. Vaccine preparation is generally described in Vaccine Design (“The subunit and adjuvant approach” (eds Powell M. F. &amp; Newman M. J.) (1995) Plenum Press New York). Encapsulation within liposomes is described by Fullerton, U.S. Pat. No. 4,235,877. 
     In one aspect of the invention is provided a vaccine kit, comprising a vial containing an immunogenic composition of the invention, optionally in lyophilised form, and further comprising a vial containing an adjuvant as described herein. It is envisioned that in this aspect of the invention, the adjuvant will be used to reconstitute the lyophilised immunogenic composition. 
     A further aspect of the invention is a method of preventing or treating  C. difficile  infection comprising administering to the host an immunoprotective dose of the immunogenic composition or vaccine or kit of the invention. In one embodiment there is provided a method of preventing or treating primary and/or recurrence episodes of  c. difficile  infection comprising administering to the host an immunoprotective dose of the immunogenic composition or vaccine or kit of the invention. 
     In one embodiment of the invention there is provided an immunogenic composition or vaccine of the invention for use in the treatment or prevention of  C. difficile  disease. In a further embodiment of the invention there is provided an immunogenic composition or vaccine of the invention for use in the treatment or prevention of disease caused by a strain of  C. difficile  selected from the group consisting of 078, 019, 023, 027, 033, 034, 036, 045, 058, 059, 063, 066, 075, 078, 080, 111, 112, 203, 250 and 571. Preferably the strain is strain 078. 
     In a further aspect of the invention there is provided a use of an immunogenic composition or vaccine of the invention in the preparation of a medicament for the prevention or treatment of  C. difficile  disease. In a further embodiment the disease is a disease caused by a strain of  C. difficile  selected from the group consisting of 078, 019, 023, 027, 033, 034, 036, 045, 058, 059, 063, 066, 075, 078, 080, 111, 112, 203, 250 and 571. Preferably the strain is strain 078. 
     In a further aspect of the invention there is provided a method of preventing or treating  C. difficile  disease comprising administering the immunogenic composition of the invention or the vaccine of the invention to a mammalian subject such as a human subject. In a further embodiment the disease is a disease caused by a strain of  C. difficile  selected from the group consisting of 078, 019, 023, 027, 033, 034, 036, 045, 058, 059, 063, 066, 075, 078, 080, 111, 112, 203, 250 and 571. Preferably the strain is strain 078. 
     General 
     Around” or “approximately” are defined as within 10% more or less of the given figure for the purposes of the invention. 
     The terms “comprising”, “comprise” and “comprises” herein are intended by the inventors to be optionally substitutable with the terms “consisting of”, “consist of” and “consists of”, respectively, in every instance. The term “comprises” means “includes.” Thus, unless the context requires otherwise, the word “comprises,” and variations such as “comprise” and “comprising” will be understood to imply the inclusion of a stated compound or composition (e.g., nucleic acid, polypeptide, antigen) or step, or group of compounds or steps, but not to the exclusion of any other compounds, composition, steps, or groups thereof. The abbreviation, “e.g.” is derived from the Latin exempli gratia, and is used herein to indicate a non-limiting example. Thus, the abbreviation “e.g.” is synonymous with the term “for example.” 
     The amino acid numbering used herein is derived from the sequences for CDTa, CDTb, Toxin A and Toxin B presented herein as SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 31 and SEQ ID NO: 32 which are to be considered as reference sequences for these proteins. 
     Embodiments herein relating to “vaccine compositions” of the invention are also applicable to embodiments relating to “immunogenic compositions” of the invention, and vice versa. 
     Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Definitions of common terms in molecular biology can be found in Benjamin Lewin, Genes V, published by Oxford University Press, 1994 (ISBN 0-19-854287-9); Kendrew et al. (eds.),  The Encyclopedia of Molecular Biology,  published by Blackwell Science Ltd., 1994 (ISBN 0-632-02182-9); and Robert A. Meyers (ed.),  Molecular Biology and Biotechnology: a Comprehensive Desk Reference,  published by VCH Publishers, Inc., 1995 (ISBN 1-56081-569-8). 
     The singular terms “a,” “an,” and “the” include plural referents unless context clearly indicates otherwise. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. The term “plurality” refers to two or more. It is further to be understood that all base sizes or amino acid sizes, and all molecular weight or molecular mass values, given for nucleic acids or polypeptides are approximate, and are provided for description. Additionally, numerical limitations given with respect to concentrations or levels of a substance, such as an antigen, may be approximate. 
     All references or patent applications cited within this patent specification are incorporated by reference herein in their entirety. 
     In order that this invention may be better understood, the following examples are set forth. 
     These examples are for purposes of illustration only, and are not to be construed as limiting the scope of the invention in any manner. 
     EXAMPLES 
     The AS01B adjuvant referred to is an adjuvant having 50 μg QS21 presented in the form of a liposome, 50 μg 3D-MPL, 0.25 mg cholesterol and 1.0 mg DOPC per 0.5 ml dose. A dose of 50 μl suitable for immunizing mice contains 5 μg QS21, 5 μg 3D-MPL, 0.025 mg cholesterol and 0.1 mg DOPC. 
     Example 1 
     Design of Binary Toxin Antigens 
     The Binary Toxin (other name: ADP-ribosyltransferase toxin) is composed by two components : the enzymatic component, named CDTa and the transport and binding component, named CDTb. 
     Based on literature data and the known 3D structure of CDTa ( J. Biol. Chem.  2009, vol. 284: 28713-28719), this protein could be divided into two domains. The N-terminal domain binds to CDTb and the C-terminal domain contains the enzymatic activity. Both domains are linked by a flexible peptide. 
     Based on literature data and information available for other B components of other bacterial binary toxins, CDTb could be divided into five domains. The first one is the prodomain, its cleavage by an enzyme having a chymotrypsin activity allows the heptamerization of the mature protein. The second domain allows the binding to CDTa. The third and fourth ones are involved in the oligomerisation and membrane insertion. Finally, the last domain is the host cell receptor binding domain. 
     Example 1a 
     Design of CDTa Antigens 
     In order to be allowed to work with CDTa and CDTb together, CDTa must be inactivated. Two possibilities of inactivation were evaluated. The first one is the design of CDTa mutants that abolish the enzymatic activity and the second one is the use of the N-terminal domain of CDTa alone. This latter domain allows the binding to CDTb and does not contain residue involved in the enzymatic activity. 
     The first set of mutants were designed based on literature information ( Infection  &amp;  Immunity,  2001, vol. 69:6004-6011). Authors demonstrated that CDTa mutant proteins E428Q, E430Q, S388A and R345K have a significant reduced activity. Based on data shown in the publication, two mutations were preferred amongst the four : CDTa mutant E428Q and E430Q. In the publication, these mutants abolish completely the CDTa enzymatic activity. In order to rank these mutants, some structural analyses were performed for these residues: surface-accessibility of the residues glutamate 428 (E428) and glutamate 430 (E430), effect of their mutations on the surrounding 3D structure. Based on these analyses, the CDTa mutant E428Q was chosen as preferred mutation and the CDTa mutant E430Q was selected as second choice. A double mutant E428Q, E430Q was also generated in order to be sure that the enzymatic activity was abolished. 
     A second set of mutants was designed because the first cytotoxicity results, obtained with the first set of mutants, were not convincing. 
     In this second set, a CDTa mutant containing 7 mutations (including the two mutations already described) was designed. All these mutations were designed based on literature information (available for CDTa or its Clostridium perfringens homologs la) and 3D structure analysis. All mutated residues are located around the catalytic site of CDTa. These residues have been modified in order to avoid ligand or water molecule binding. This CDTa “super mutant” contains the mutations R345A, Q350A, N385A, R402A, S388F, E428Q and E430Q. Based on this “super mutated” CDTa, two other CDTa mutated variants were evaluated in order to eliminate E428Q and E430Q mutations (construct C108 contains the E430Q mutation but not the mutation E428Q, construct C107 does not contain both mutations). 
     CDTa Nterminal Domain Alone 
     It was described in the literature (Infection &amp; Immunity, 2001, vol. 69:6004-6011) that the CDTa 1-240  is the minimal CDTa fragment that still allows a binding to lb (B component of binary toxin of  Clostridium perfringens ). This fragment will be tested in the lab but based on known 3D structure, it was suggested that this domain will probably not be optimal in term of correct folding of this domain of CDTa. 
     Antigen design was performed based on the known 3D structure (Protein Data Bank accession number: 2WN4,  J. Biol. Chem.,  2009, vol. 284:28713-28719) to improve the expression and folding of an isolated CDTa N-terminal domain. On the 3D structure, a linker peptide of eight amino acids allows the separation between the N- and C-terminal domains of CDTa. Two isolated CDTa N-terminal domains were designed, the first one contains this flexible peptide and the second one not. 
     CDTa: Sequences Summary 
     A summary of all CDTa sequences is presented in table 1. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                   
                 Length 
                   
                   
               
               
                 Name 
                 (aa)* 
                 Location 
                 Comments 
               
               
                   
               
             
            
               
                 CDTa 
                 463 
                  1-463 
                 Full length of CDTa coming from strain 
               
               
                   
                   
                   
                 R20291 
               
               
                 CDTa′ 
                 421 
                 44-463 
                 CDTa without signal peptide (C34) 
               
               
                 CDTa_E428Q 
                 421 
                 44-463 
                 CDTa′ with mutation of Glu 428  into Gln (C44) 
               
               
                 CDTa_E430Q 
                 421 
                 44-463 
                 CDTa′ with mutation of Glu 430  into Gln (C54) 
               
               
                 CDTa_E428 430Q 
                 421 
                 44-463 
                 CDTa′ with the two mutations Glu 428  into Gln 
               
               
                   
                   
                   
                 and Glu 430  into Gln (C67) 
               
               
                 CDTa_7mutations 
                 421 
                 44-463 
                 CDTa′ containing 7 mutated amino acids 
               
               
                   
                   
                   
                 (C69) 
               
               
                 CDTa_N_litt 
                 198 
                 44-240 
                 Minimum CDTa N-terminal domain that still 
               
               
                   
                   
                   
                 allows binding to Ib (C51) 
               
               
                 CDTa_NADlink 
                 226 
                 44-268 
                 CDTa N-terminal domain based on antigen 
               
               
                   
                   
                   
                 design work (C49) 
               
               
                 CDTa_NAD 
                 218 
                 44-260 
                 CDTa N-terminal domain based on antigen 
               
               
                   
                   
                   
                 design work (C50) 
               
               
                   
               
               
                 *Length contains additional N-terminal Methionine but not the His-tag 
               
            
           
         
       
     
     Example 1b 
     Design of CDTb Antigens 
     CDTb Mature 
     In order to avoid the chymotrypsin activation step in the CDTb process, it was tried to express only the mature CDTb protein (without its signal peptide and prodomain). In the literature ( Protein Expression and Purification,  2010, vol. 74 : 42-48), the mature CDTb was described as starting at Leucine 210. This mature CDTb was named CDTb“. After in house experimental data, it seems that the activated CDTb starts at Serine 212. This result was supported by analysis of a 3D modelised structure of CDTb. This model was built using SwissModel ( Bioinformatics,  2006, vol. 22:195-201). The template used for the homology modeling was the B component of  Bacillus anthracia,  named Protective Antigen or PA (Protein Data Bank accession number : 3TEW). 
     CDTb Receptor-Binding Domain Alone 
     Given the fact that a fusion containing only the receptor-binding domains of Toxin A and B is sufficient to induce neutralizing antibodies, it was decided to produce and evaluate the CDTb receptor-binding domain alone. 
     The 3D structure model obtained for CDTb is accurate for the four first domains of CDTb but not for the receptor-binding domain (these domains of CDTb and PA are too different). To design constructs expressing this domain alone, the C-terminal part of the fourth domain was analysed on the 3D structure model in order to decide where the last domain will start. Two versions of the CDTb-receptor binding domain were designed. In the first one, this domain starts just after the modelised 3D structure of the fourth domain. In this version, the CDTb-receptor-binding domain will probably have a long flexible peptide in its N-terminal part. The second version starts where the 2D predicted structures performed on the C-terminal part of CDTb (predictions done using Psipred program,  Bioinformatics,  2000, vol. 16:404-405) become more compacts after a lack of predicted secondary structures. This could indicate the beginning of a new structural domain. In this second version, no flexible peptide is present at the N-terminal part of the isolated CDTb receptor-binding domain. 
     CDTb Ca 2+  Binding Motif Mutation 
     Following literature information, mutations in the Ca 2+  binding domain of the B component of Iota toxin of  Clostridium perfringens  (Ib) abolish the binding with the A component of this binary toxin (Ia). These mutations could be very interesting in the case of a vaccine composition containing a mixture of mature CDTb protein and a wild type CDTa protein. Using multiple protein sequence alignment, these mutations were located on the CDTb sequence and mutated. It concerns residues Asp 220 , Asp 222  and Asp 224 . They were mutated into Ala residues. 
     CDTb Prodomain 
     In order to try to decrease the degradation issues observed with C55 in gel, some co-expression tests were evaluated. The working hypothesis of doing that is to improve the folding of the mature CDTb. 
     Two limits of prodomain were proposed. The first one starts at residue 43 of CDTb (after the signal peptide cleavage) and finishes at residue Met 211  (given that the experimentally determined first residue of the mature CDTb is Ser 212 ). The second prodomain was designed based on the predicted 3D structure of CDTb. The linker existing between the prodomain and the first structural domain of the mature CDTb protein is removed in this construct. 
     CDTb: Sequence Summary 
     A summary of all CDTb sequences is presented in table 2. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                   
                 Length 
                   
                   
               
               
                 Name 
                 (aa)* 
                 Location 
                 Comments 
               
               
                   
               
             
            
               
                 CDTb 
                 876 
                  1-876 
                 Full length of CDTb coming from strain 
               
               
                   
                   
                   
                 R20291 
               
               
                 CDTb′ 
                 835 
                  43-876 
                 CDTb without signal peptide (C38) 
               
               
                 CDTb″ 
                 668 
                 210-876 
                 CDTb without signal peptide and prodomain 
               
               
                   
                   
                   
                 as defined in the literature 
               
               
                 CDTb″_xp data 
                 666 
                 212-876 
                 CDTb without signal peptide and prodomain, 
               
               
                   
                   
                   
                 as demonstrated by in house experimental 
               
               
                   
                   
                   
                 results (C55) 
               
               
                 CDTbClg 
                 258 
                 620-876 
                 CDTb receptor-binding domain containing 
               
               
                   
                   
                   
                 natural flexible peptide in its N-terminal part, 
               
               
                   
                   
                   
                 based on antigen design work (C52) 
               
               
                 CDTbCsh 
                 242 
                 636-876 
                 CDTb receptor-binding domain, based on 
               
               
                   
                   
                   
                 antigen design work (C53) 
               
               
                 CDTb Ca2+ 
                 666 
                 212-876 
                 Mature CDTb (without signal peptide and 
               
               
                 mutated 
                   
                   
                 without prodomain) containing 3 mutations 
               
               
                   
                   
                   
                 D220A, D222A and D224A (C97) 
               
               
                 CDTbprodomainLg 
                 170 
                  43-211 
                 CDTb prodomain (C58) 
               
               
                 CDTbprodomainSh 
                 145 
                  43-186 
                 CDTb prodomain without the linker existing 
               
               
                   
                   
                   
                 between the prodomain and the first 
               
               
                   
                   
                   
                 structural domain of mature CDTb (C59) 
               
               
                   
               
               
                 *Length contains additional N-terminal Methionine but not the His-tag 
               
            
           
         
       
     
     Example 1c 
     Design of CDTa-CDTb Fusions 
     Background Information 
     The aim of these constructs is to obtain both components of the Binary Toxin into one process. 
     A lot of different kinds of fusions could be designed but, as proof of concept, the first fusion evaluated is the combination of CDTa N-terminal domain (named CDTaNADlink and CDTaNAD) with CDTb receptor-binding domain (named CDTbCsh and CDTbClg). 
     Fusion CDTaNterm—CDTb Receptor-Binding Domain Alone 
     Without additional experimental data on each partner of the fusion, all possible combinations were initiated but always with the CDTa domain as first partner of the fusion. 
     In these fusions, the CDTaNADlink and the CDTaNAD domains have two and one residues less than the designed isolated domains, respectively. These CDTa additional amino acids were kept in the isolated designs in order to avoid potential issues during the expression process. 
     A summary of all CDTa-CDTb fusion sequences is presented in table 3. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 3 
               
             
            
               
                   
                   
               
               
                   
                 Length 
                 Location 
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 Name 
                 (aa)* 
                 CDTa 
                 CDTb 
               
               
                   
                   
               
               
                   
                 CDTaNADlink-CDTbCsh 
                 465 
                 44-266 
                 636-876 
               
               
                   
                 (C61) 
               
               
                   
                 CDTaNAD-CDTbCsh 
                 458 
                 44-259 
                 636-876 
               
               
                   
                 (C63) 
               
               
                   
                 CDTaNADlink-CDTbClg 
                 481 
                 44-266 
                 620-876 
               
               
                   
                 (C60) 
               
               
                   
                 CDTaNAD-CDTbClg 
                 474 
                 44-259 
                 620-876 
               
               
                   
                 (C62) 
               
               
                   
                   
               
               
                   
                 *Length contains additional N-terminal Methionine but not the His-tag 
               
            
           
         
       
     
     Example 1d 
     Design of ToxA-ToxB—CDTb Receptor-Binding Domain Fusion 
     The objective of this fusion is the combination of receptor-binding domains of the three major toxins of  Clostridium difficile  into one construct. 
     Given the fact that F2 and CDTb receptor-binding domains are not supposed to adopt the same fold, a linker/spacer must be used between the two partners of the fusion in order to allow a correct independent folding of them. Two fusions were designed. 
     In the first one (named F2_CDTbClg), the long designed version of the receptor-binding domain is fused at the C-terminal part of F2. In this version, the long flexible N-terminal peptide of CDTb receptor-binding domain will function as spacer. 
     In the second fusion (named F2_GG_NVCDTbCsh), the short designed version of the receptor-binding domain is fused at the C-terminal part of F2. In order to allow a correct folding of the two partners, the length of the linker created in this fusion must be increased. In order to do that, the CDTb-receptor binding domain was extended with two natural residues, moreover two exogenous Glycines were added between F2 and the longer version of CDTbCsh. 
     A summary of all F2-CDTb fusion sequences is presented in table 4. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 4 
               
             
            
               
                   
                   
               
               
                   
                 Length 
                 Location 
                   
               
            
           
           
               
               
               
               
               
            
               
                 Name 
                 (aa)* 
                 F2 
                 CDTb 
                 Comments 
               
               
                   
               
               
                 F2_CDTbClg 
                 1223 
                 ToxA: 2121-2686 
                 620-876 
                   
               
               
                   
                   
                 ToxB: 
               
               
                   
                   
                 1968-2366 
               
               
                 F2_GG_NVCDTbCsh 
                 1211 
                 ToxA: 2121-2686 
                 634-876 
                 Two exogenous Gly residues 
               
               
                   
                   
                 ToxB: 
                   
                 added as spacer between the 
               
               
                   
                   
                 1968-2366 
                   
                 two partners of the fusion 
               
               
                   
               
               
                 *Length contains additional N-terminal Methionine but not the His-tag 
               
            
           
         
       
     
     Example 2 
     Cloning, Expression and Purification of CdtA Protein 
     Expression Plasmid and Recombinant Strain: CdtA Full Length 
     Genes encoding the protein of full length without signal peptide of CdtA with and without mutations (see tables below) and a His tag in C-term were cloned into the pET24b(+) expression vector (Novagen) using the Ndel/Xhol restriction sites using standard procedures. Final constructs were generated by the transformation of  E. coli  strain HMS174 (DE3) or BLR (DE3) pLysS (C34) with each recombinant expression vector separately according to standard method with CaCl2-treated cells (Hanahan D. &lt;&lt;Plasmid transformation by Simanis.&gt;&gt; In Glover, D. M. (Ed), DNA cloning. IRL Press London. (1985): p. 109-135.). 
     CdtA 
     C Number 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 C34 
                 CdtA (aa44-463) 
               
               
                   
                 C44 
                 CdtA (aa44-463) mut. E428Q 
               
               
                   
                 C49 
                 CdtA linker (44-268) 
               
               
                   
                 C50 
                 CdtA WO linker (44-260) 
               
               
                   
                 C54 
                 CdtA (aa44-463) mut. E430Q 
               
               
                   
                 C67 
                 CdtA (aa44-463) mut. E428Q-E430Q 
               
               
                   
                 C68 
                 CdtA (aa44-463) mut. R345A-Q350A-N385A- 
               
               
                   
                   
                 R402A-E428Q-E430Q 
               
               
                   
                 C69 
                 CdtA (aa44-463) mut. R345A-Q350A-N385A- 
               
               
                   
                   
                 R402A-S388F-E428Q-E430Q 
               
               
                   
                 C107 
                 CdtA (aa44-463) mut. R345A-Q350A-N385A- 
               
               
                   
                   
                 R402A-S388F 
               
               
                   
                 C108 
                 CdtA (aa44-463) mut. R345A-Q350A-N385A- 
               
               
                   
                   
                 R402A-S388F-E430Q 
               
               
                   
                 C110 
                 CdtA (aa44-463) mut. R345A-Q350A-N385A- 
               
               
                   
                   
                 R402A-S388F-E428Q 
               
               
                   
                   
               
            
           
         
       
     
     Host Strain: 
     HMS 174 (DE3). HMS174 strains provide the recA mutation in a K-12 background. Strains having the designation (DE3) are lysogenic for a A prophage that contains an IPTG inducible T7 RNA polymerase. A DE3 lysogens are designed for protein expression from pET vectors Genotype: F recA1 hsdR(r K12   − m K12   + ) (Rif R ). 
     BLR(DE3) pLysS. BLR is a recA derivative of BL21. Strains having the designation (DE3) are lysogenic for a A prophage that contains an IPTG inducible T7 RNA polymerase. λ DE3 lysogens are designed for protein expression from pET vectors This strain is also deficient in the Ion and ompT proteases, pLysS strains express T7 lysozyme wich further supress basal expression of the T7 RNA polymerase prior to induction. 
     Genotype :  E. coli  BLR::DE3 strain, F −  ompT hsdS B (r B   − m B   − ) gal dcm (DE3) Δ(srl-recA)306::Tn10 pLysS (Cam R , Tet R ). 
     Expression of the Recombinant Proteins: 
       E. coli  transformants were stripped from agar plate and used to inoculate 200 ml of LBT broth ±1% (w/v) glucose+kanamycin (50 μg/ml) to obtain O.D.600 nm between 0.1-0.2. Cultures were incubated overnight at 37° C., 250 RPM. 
     Each overnight culture were diluted to 1:20 in 500 ml of LBT medium containing kanamycin (50 μg/ml) and grown at 37° C. at a stirring speed of 250 rpm until O. D.620 reached 0.5/0.6. 
     At O.D.600 nm around 0.6, the cultures were cooled down before inducing the expression of the recombinant protein by addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; EMD Chemicals Inc., catalogue number: 5815) and incubated overnight at 23° C., 250 RPM. 
     After overnight induction (around 16 hours), O. D. 600nm  were evaluated after induction and culture were centrifuged at 14 000 RPM for 15 minutes and pellets were frozen at −20° C. separately. 
     Expression Plasmid and Recombinant Strain: CdtA—N-Term 
     Genes encoding protein of N-terminal, without signal peptide of CdtA (see tables below) and a His tag in C-term were cloned into the pET24b(+) expression vector (Novagen) using the Ndel/Xhol restriction sites using standard procedures. Final constructs were generated by the transformation of  E. coli  strain HMS174 (DE3) with each recombinant expression vectors separately according to standard method with CaCl2-treated cells (Hanahan D. &lt;&lt;Plasmid transformation by Simanis. &gt;&gt; In Glover, D. M. (Ed), DNA cloning. IRL Press London. (1985): p. 109-135.). 
     CdtA N-Term 
     C Number 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 C49 
                 CdtA linker (44-268) 
               
               
                   
                 C50 
                 CdtA WO linker (44-260) 
               
               
                   
                   
               
            
           
         
       
     
     Host Strain: 
     HMS 174 (DE3). HMS174 strains provide the recA mutation in a K-12 background. Strains having the designation (DE3) are lysogenic for a A prophage that contains an IPTG inducible T7 RNA polymerase. A DE3 lysogens are designed for protein expression from pET vectors 
     Genotype: recA1 hsdR(r K12   − m K12   + ) (Rif R ). 
     Expression of the Recombinant Proteins: 
       E. coli  transformants were stripped from agar plate and used to inoculate 200 ml of LBT broth ±1% (w/v) glucose+kanamycin (50 μg/ml) to obtain O.D.600 nm between 0.1-0.2. Cultures were incubated overnight at 37° C., 250 RPM. 
     This overnight culture was diluted to 1:20 in 500 ml of LBT medium containing kanamycin (50 μg/ml) and grown at 37° C. at a stirring speed of 250 rpm until O.D.620 reached 0.5/0.6. 
     At O.D.600 nm around 0.6. the culture was cooled down before inducing the expression of the recombinant protein by addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; EMD Chemicals Inc., catalogue number: 5815) and incubated overnight at 23° C., 250 RPM. 
     After overnight induction (around 16 hours), O.D. 600nm  was evaluated after induction and culture was centrifuged at 14 000 RPM for 15 minutes and pellets were frozen at −20° C. separately. 
     Purification 
     The following procedure was used to purify constructs C34, C44, C49, C50, C54, C67, C69, C107 and C110. 
     The bacterial pellets were re-suspended in 20 mM or 50 mM bicine buffers (pH 7.5 or pH 8.0), containing 500 mM NaCl , 0 mM or 5 mM TCEP (Thermo Scientific Pierce, (2-carboxyethyl)phosphine hydrochloride) and a mixture of protease inhibitors (Complete, Roche, without EDTA). Bacteria were lysed using a French Press system 3×20 000 PSI. Soluble (supernatant) and insoluble (pellet) components were separated by centrifugation at 20 000 g for 30 min at 4° C. 
     The 6-His tagged-proteins were purified under native conditions on IMAC. The soluble components were loaded on a 5 ml GE Histrap column (GE) pre-equilibrated with the same buffer used to bacterial re-suspension. After loading on the column, the column was washed with a 20 mM or 50 mM bicine buffer (pH7.5 or pH8.0), containing 500 mM NaCl, 10 mM imidazole, 5 mM TCEP. Elution was performed using a 50 mM bicine buffer pH7.6, 500 mM NaCl, 1 mM TCEP and imidazole (250 mM or 500 mM). 
     After desalting (BIORAD Bio-Gel P6 Desalting) and concentration (Amicon Ultra 10 kDa) steps, the product was loaded on SEC chromatography (SUPERDEX™ 75 or 200) in 20 mM or 50 mM bicine buffer(pH7.5 or pH8.0), 150 mM NaCl, 1m M TCEP, for further purification step. 
     Fractions containing Cdta antigen were selected on the basis of purity by SDS-PAGE. Protein concentration was determined using Lowry RC/DC Protein Assay of BioRad. The purified bulk was sterile-filtered on 0.22 μm and stored at −80° C. 
     Example 4 
     Cloning, Expression and Purification of  C. difficile  CdtB Protein 
     Expression Plasmid and Recombinant Strain: CdtB Full Length. 
     Genes encoding the truncated protein of CdtB without signal peptide (Pro-CdtB′) and a His tag in C-term were cloned into the pGEX-6 μl expression vector (GE Healthcare) using the BamHl/Xhol restriction sites using standard procedures. This vector included GST (Gluthathione-S-transferase) as fusion partner in N-terminal of either CdtB′(GST-Pro-Cdtb′). The final construct was generated by the transformation of  E. coli  strain BL21 (DE3) with the recombinant expression vector according to standard method with CaCl2-treated cells (Hanahan D. &lt;&lt;Plasmid transformation by Simanis. &gt;&gt; In Glover, D. M. (Ed), DNA cloning. IRL Press London. (1985): p. 109-135.). 
     Genes encoding the truncated protein of CdtB without signal peptide (Pro-CdtB′: C38) and without signal peptide and prodomain (CdtB″: C40 or C55) and a His tag in C-term were cloned into the pET24b(+) expression vector (Novagen) using the Ndel/Xhol restriction sites using standard procedures. Final constructs were generated by the transformation of  E. coli  B834 (DE3) modified strain for C55 and BL21(DE3) for C38 and C40 with the appropriate recombinant expression vectors according to standard method with CaCl2-treated cells (Hanahan D. &lt;&lt;Plasmid transformation by Simanis. &gt;&gt; In Glover, D. M. (Ed), DNA cloning. IRL Press London. (1985): p. 109-135.). 
     CdtB 
     C Number 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 C37 
                 CdtB′ Δsignal sequence (aa43-876) + 
               
               
                   
                   
                 GST N-term 
               
               
                   
                 C38 
                 CdtB′ Δsignal sequence (aa43-876) 
               
               
                   
                 C40 
                 CdtB″ Δ signal sequence and 
               
               
                   
                   
                 prodomain (aa210-876) 
               
               
                   
                 C55 
                 CdtB Δ prodomain (aa. 212-876) 
               
               
                   
                   
               
            
           
         
       
     
     Host Strain 
     BL21(DE3). BL21(DE3) is a non-methionine auxotroph derivative of B834. Strains having the designation (DE3) are lysogenic for a A prophage that contains an IPTG inducible T7 RNA polymerase. A DE3 lysogens are designed for protein expression from pET vectors This strain is also deficient in the Ion and ompT proteases. 
     Genotype :  E. coli  BL21::DE3 strain, F −  ompT hsdS B (r B   − m B   − ) gal dcm (DE3). 
     B834 is the parental strain for BL21. These protease-deficient hosts are methionine auxotrophs. A DE3 lysogens are designed for protein expression from pET vectors This strain is also deficient in the Ion and ompT proteases. 
     Modification: Including PGL gene to avoid phosphogluconoylation in the biotin locus (Strain is auxotroph for biotin). 
     Genotype : B834 ::DE3 strain, F-ompT hsdSB(rB− mB−) gal dcm met (DE3) 
     Modification: Δ(bioA-bioD)::PGL 
     Expression of the Recombinant Proteins: 
       E. coli  transformants were stripped from agar plate and used to inoculate 200 ml of LBT broth ±1% (w/v) glucose +/− kanamycin (50 μg/ml) or ampicillin (100 μg/ml) to obtain O.D. 600nm  between 0.1-0.2. Cultures were incubated overnight at 37° C., 250 RPM. 
     Overnight culture were diluted to 1:20 in 500 ml of LBT medium containing +/− kanamycin (50 μg/ml) or ampicillin (100 μg/ml) and grown at 37° C. at a stirring speed of 250 rpm until O.D. 620  reached 0.5/0.6. 
     At an O.D. at 600 nm of around 0.6, cultures were cooled down before inducing the expression of the recombinant protein by addition of 1 mM isopropyl p-D-1-thiogalactopyranoside (IPTG; EMD Chemicals Inc., catalogue number: 5815) and incubated overnight at 23° C., 250 RPM. 
     After the overnight inductions (around 16 hours), O.D. at 600 nm were evaluated after induction and cultures were centrifuged at 14 000 RPM for 15 minutes and pellets were frozen at −20° C. separately. 
     Expression Plasmid and Recombinant Strain. 
     Genes encoding the truncated protein of CdtB without prodomain CdtB mature, Knock-out of Ca++ bindinding site (inhibit binding of CdtA to CdtB) and a His tag in C-term was cloned into the pET24b(+) expression vector (Novagen) using the Ndel/Xhol restriction sites using standard procedures. The final construct was generated by the transformation of  E. coli  B834 (DE3) modified strain with the recombinant expression vector according to standard method with CaCl2-treated cells (Hanahan D. &lt;&lt;Plasmid transformation by Simanis.&gt; In Glover, D. M. (Ed), DNA cloning. IRL Press London. (1985): p. 109-135.). 
     CdtB Ca++ Binding Domain Knock-Out 
     C Number 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 C97 
                 CdtB mature mut Asp 220-222-224 Ala 
               
               
                   
                   
                 (aa. 212-876) 
               
               
                   
                   
               
            
           
         
       
     
     Host Strain 
     B834 is the parental strain for BL21. These protease-deficient hosts are methionine auxotrophs. A DE3 lysogens are designed for protein expression from pET vectors This strain is also deficient in the Ion and ompT proteases. 
     Modification: Including PGL gene to avoid phosphogluconoylation in the biotin locus (Strain is auxotroph for bioti n). 
     Genotype: B834 ::DE3 strain, F-ompT hsdSB(rB− mB−) gal dcm met (DE3) 
     Modification: Δ(bioA-bioD)::PGL 
     Expression of the Recombinant Proteins: 
     A  E coli  transformant was stripped from agar plate and used to inoculate 200 ml of LBT broth ±1% (w/v) glucose+kanamycin (50 μg/ml) to obtain O.D. 600nm  between 0.1-0.2. Cultures were incubated overnight at 37° C., 250 RPM. 
     This overnight culture was diluted to 1:20 in 500 ml of LBT medium containing kanamycin (50 μg/ml) and grown at 37° C. at a stirring speed of 250 rpm until O.D. 620  reached 0.5/0.6. 
     At an O.D.at 600 nm of around 0.6, the culture was cooled down before inducing the expression of the recombinant protein by addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; EMD Chemicals Inc., catalogue number: 5815) and incubated overnight at 23° C., 250 RPM. 
     After the overnight induction (around 16 hours). O.D. at 600 nm was evaluated after induction and culture was centrifuged at 14 000 RPM for 15 minutes and pellets were frozen at −20° C. separately. 
     Purification 
     C37 
     The bacterial pellet was re-suspended in 50 mM bicine buffer (pH 8.0) containing 500 mM NaCl, 5 mM TCEP (Thermo Scientific Pierce, (2-carboxyethyl)phosphine hydrochloride) and a mixture of protease inhibitor (Complete, Roche). Bacteria were lysed using a French Press system 
     3×20 000 PSI. Soluble (supernatant) and insoluble (pellet) components were separated by centrifugation at 20 000 g for 30 min at 4° C. 
     The 6-His tagged-protein was purified under native conditions on IMAC. The soluble components were loaded on a 5 ml GE Histrap column (GE) pre-equilibrated with the same buffer used to bacterial re-suspension. After loading on the column, the column was washed with a 50 mM bicine buffer pH8.0, containing 150 mM NaCl, 25 mM imidazole, 1 mM TCEP. Elution was performed using a 50 mM bicine buffer pH8.0 containing 150 mM NaCl, 250 mM imidazole, 1 mM TCEP. 
     After desalting step (BIORAD Bio-Gel P6 Desalting) in 50 mM bicine buffer pH8.0 containing 150 mM NaCl and 1 mM TCEP, the product was treated (overnight at 4° C.) with PreScission protease (GE-Healthcare) in order to cleave the GST tag. After overnight treatment, 0.2% Tween 20 was added to the digestion mixture. 
     Then the protein was passed through a GST affinity column (GE GSTrap FF) pre-equilibrated with buffer 50 mM bicine buffer pH8.0 containing 150 mM NaCl , 1 mM TCEP, 0.2% tween20 and 20 mM reduced glutation, in order to remove the cleaved tag, un-cleaved fusion protein and the PreScission protease. 
     The GST-free protein was collected in the flow through and loaded again on a 5 ml GE Histrap column (GE) pre-equilibrated with 50 mM bicine buffer pH8.0 containing 150 mM NaCl, 1 mM TCEP, 0.2% tween20. After loading on the column, the column was washed with a 50 mM bicine buffer pH8.0, containing 150 mM NaCl, 0.2% tween20, 1 mM TCEP and 10 mM imidazole. Elution was performed using a 50 mM bicine buffer pH8.0 containing 150 mM NaCl, 0.2% tween20, 1 mM TCEP and 500 mM imidazole. 
     After desalting step (BIORAD Bio-Gel P6 Desalting) in 50 mM bicine buffer pH8.0 containing 150 mM NaCl, 1 mM TCEP and 0.2% tween 20, the product was treated with a-chymotrypsin (from bovine pancreas—Sigma), followed by trypsin inhibitor treatment (from egg white—Sigma). The complete activation of Cdtb by chymotrypsin was monitored by SDS-PAGE. 
     The fully activated product was loaded on SEC chromatography (SUPERDEX™ 75) in 50 mM bicine buffer pH8.0 containing 300 mM NaCl, 1 mM TCEP. Fractions containing CdtB antigen were selected on the basis of purity by SDS-PAGE. Protein concentration was determined using Lowry RC/DC Protein Assay of BioRad. The purified bulk was sterile-filtered on 0.22 μm and stored at −80° C. 
     C38 
     The bacterial pellet was re-suspended in 50 mM bicine buffer (pH 8.0) containing 150 mM NaCl , 5 mM TCEP (Thermo Scientific Pierce, (2-carboxyethyl)phosphine hydrochloride), 0.4%empigen and a mixture of protease inhibitor (Complete, Roche). Bacteria were lysed using a French Press system 3&gt;20 000 PSI. Soluble (supernatant) and insoluble (pellet) components were separated by centrifugation at 20 000 g for 30 min at 4° C. 
     The 6-His tagged-protein was purified under native conditions on IMAC. The soluble components were loaded on a 5 ml GE Histrap column (GE) pre-equilibrated with 50 mM bicine buffer (pH 8.0) containing 150 mM NaCl , 1 mM TCEP (Thermo Scientific Pierce, (2-carboxyethyl)phosphine hydrochloride) and 0.15% empigen. After loading on the column, the column was washed with a 50 mM bicine buffer pH8.0, containing 150 mM NaCl, 20 mM imidazole, 1 mM TCEP and 0.2% tween 20. Elution was performed using a 50 mM bicine buffer pH8.0 containing 150 mM NaCl, 500 mM imidazole, 1 mM TCEP and 0.2% tween 20. 
     After desalting step (BIORAD Bio-Gel P6 Desalting) in 50 mM bicine buffer pH8.0 containing 150 mM NaCl, 1 mM TCEP and 0.2% tween 20, the product was treated with α-chymotrypsin (from bovine pancreas—Sigma), followed by trypsin inhibitor treatment (from egg white—Sigma). The complete activation of Cdb by chymotrypsin was monitored by SDS-PAGE. 
     The fully activated product was loaded on SEC chromatography (SUPERDEX ™ 75) in 50 mM bicine buffer pH8.0, 300 mM NaCl, 1 mM TCEP. Fractions containing Cdtb protein were selected on the basis of purity by SDS-PAGE and loaded again on a 5 ml GE Histrap column (GE) pre-equilibrated with 50 mM bicine buffer (pH 8.0) containing 300 mM NaCl, 1 mM TCEP. After loading on the column, the column was washed with a 50 mM bicine buffer pH8.0, containing 300 mM NaCl, 10 mM imidazole, 1 mM TCEP. Elution was performed using a 50 mM bicine buffer pH8.0 containing 300 mM NaCl, 500 mM imidazole, 1 mM TCEP. 
     After desalting step (BIORAD Bio-Gel P6 Desalting) in 50 mM bicine buffer pH8.0 containing 300 mM NaCl, 1 mM TCEP the protein concentration was determined using Lowry RC/DC Protein Assay of BioRad. The purified bulk was sterile-filtered on 0.22 μm and stored at −80° C. 
     C40 
     The bacterial pellet was re-suspended in 20 mM bicine buffer (pH 8.0) containing 500 mM NaCl, 5 mMCaCl 2  and a mixture of protease inhibitor (Complete, Roche). Bacteria were lysed using a French Press system 3×20 000 PSI. Soluble (supernatant) and insoluble (pellet) components were separated by centrifugation at 20 000 g for 30 min at 4° C. 
     The 6-His tagged-protein was purified under native conditions on IMAC. The soluble components were loaded on a 1 ml GE Histrap column (GE) pre-equilibrated with 20 mM bicine buffer (pH 8.0) containing 500 mM NaCl, 5 mMCaCl 2 . After loading on the column, the column was washed with a 20 mM bicine buffer pH8.0, containing 500 mM NaCl, 5 mM CaCl 2  and 5 mM imidazole. Elution was performed using a 20 mM bicine buffer pH8.0 containing 150 mM NaCl, 5 mM CaCl 2  and 250 mM imidazole. 
     After desalting step (BIORAD Bio-Gel P6 Desalting) in 20 mM bicine buffer pH8.0 containing 150 mM NaCl, 1 mM TCEP the product was loaded on SEC chromatography (SUPERDEX™ 75) in the same buffer. Fractions containing Cdtb antigen were selected on the basis of purity by SDS-PAGE. Protein concentration was determined using Lowry RC/DC Protein Assay of BioRad. The purified bulk was sterile-filtered on 0.22 μm and stored at −80° C. 
     C55 
     The bacterial pellet was re-suspended in 50 mM bicine buffer (pH 8.0) containing 150 mM NaCl. 
     5 mM TCEP (Thermo Scientific Pierce, (2-carboxyethyl) phosphine hydrochloride), 0.4% empigen and a mixture of protease inhibitors (Complete, Roche). Bacteria were lysed using a French Press system 3×20 000 PSI. Soluble (supernatant) and insoluble (pellet) components were separated by centrifugation at 20 000 g for 30 min at 4° C. 
     The 6-His tagged-protein was purified under native conditions on IMAC. The soluble components were loaded on a 5 ml GE Histrap column (GE) pre-equilibrated with 50 mM bicine buffer (pH 8.0) containing 150 mM NaCl, 0.15% empigen, 1 mM TCEP. After loading on the column, the column was washed with a 50 mM bicine buffer pH8.0, containing 150 mM NaCl, 0.2% tween 20, 20 mM imidazole and 1 mM TCEP. Elution was performed using a 50 mM bicine buffer pH8.0 containing 150 mM NaCl, 0.2% tween 20, 500 mM imidazole and 1 mM TCEP. 
     After desalting step (BIORAD Bio-Gel P6 Desalting) in 50 mM bicine buffer pH8.0 containing 300 mM NaCl, 1 mM TCEP the product was loaded on SEC chromatography (SUPERDEX™ 75) in the same buffer. Fractions containing Cdtb antigen were selected on the basis of purity by SDS-PAGE. Protein concentration was determined using Lowry RC/DC Protein Assay of BioRad. The purified bulk was sterile-filtered on 0.22 μm and stored at −80° C. 
     Expression of the Recombinant Proteins: CdtB Receptor Binding Domain: 
     Expression Plasmid and Recombinant Strain. 
     Genes encoding the truncated protein of CdtB only recptor binding domain (C52-C53) and a His tag in C-term was cloned into the pET24b(+) expression vector (Novagen) using the Ndel/Xhol restriction sites using standard procedures. The final constructs were generated by the transformation of  E. coli  B834 (DE3) modified strain with the recombinant expression vector according to standard method with CaCl2-treated cells (Hanahan D. &lt;&lt;Plasmid transformation by Simanis. &gt;&gt; In Glover, D. M. (Ed), DNA cloning. IRL Press London. (1985): p. 109-135.). 
     Host Strain 
     B834 is the parental strain for BL21. These protease-deficient hosts are methionine auxotrophs. A DE3 lysogens are designed for protein expression from pET vectors This strain is also deficient in the Ion and ompT proteases. 
     Modification: Including PGL gene to avoid phosphogluconoylation in the biotin locus (Strain is auxotroph for bioti n). 
     Genotype: B834 ::DE3 strain, F-ompT hsdSB(rB− mB−) gal dcm met (DE3) 
     Modification: L(bioA-bioD)::PGL 
     CdtB Rec. Bdng Domain 
     C Number 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 C52 
                 CdtB receptor binding domain 
               
               
                   
                   
                 long (aa. 620-876) 
               
               
                   
                 C53 
                 CdtB receptor binding domain 
               
               
                   
                   
                 short (aa. 636-876) 
               
               
                   
                   
               
            
           
         
       
     
     Expression of the Recombinant Proteins: 
     A  E. coli  transformants were stripped from agar plate and used to inoculate 200 ml of LBT broth ±1% (w/v) glucose+kanamycin (50 μg/ml) to obtain O.D. 600 nm  between 0.1-0.2. Cultures were incubated overnight at 37° C., 250 RPM. 
     These overnight cultures were diluted to 1:20 in 500 ml of LBT medium containing kanamycin (50 μg/ml) and grown at 37° C. at a stirring speed of 250 rpm until O.D. 620  reached 0.5/0.6. 
     At an O.D.at 600 nm of around 0.6, the cultures were cooled down before inducing the expression of the recombinant protein by addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; EMD Chemicals Inc., catalogue number: 5815) and incubated overnight at 23° C., 250 RPM. 
     After the overnight inductions (around 16 hours), O.D. at 600 nm were evaluated after induction and cultures were centrifuged at 14 000 RPM for 15 minutes and pellets were frozen at −20° C. separately. 
     Purification 
     C52 and C53 
     The bacterial pellets were re-suspended in 50 mM bicine buffer pH 8.0, containing 500 mM NaCl and a mixture of protease inhibitors (Complete, Roche, without EDTA). Bacteria were lysed using a French Press system 3×20 000 PSI. Soluble (supernatant) and insoluble (pellet) components were separated by centrifugation at 20 000 g for 30 min at 4° C. 
     The 6-His tagged-proteins were purified under native conditions on IMAC. The soluble components were loaded on a 5 ml GE Histrap column (GE) pre-equilibrated with the same buffer used to bacterial re-suspension. After loading on the column, the column was washed with a 20 mM bicine buffer 
     pH7.5 containing 500 mM NaCl, 25 mM imidazole. Elution was performed using a 50 mM bicine buffer pH7.5, 500 mM NaCl, and 250 mM imidazole. 
     After desalting (BIORAD Bio-Gel P6 Desalting) and concentration (Amicon Ultra 10 kDa) steps, the product was loaded on SEC chromatography (SUPERDEX ™ 75) in 20 mM buffer pH7.5, 
     150 mM NaCl. 
     Fractions containing Cdtb antigen were selected on the basis of purity by SDS-PAGE. Protein concentration was determined using Lowry RC/DC Protein Assay of BioRad. The purified bulk was sterile-filtered on 0.22 μm and stored at −80° C. 
     Example 6 
     Cloning, Expression and Purification of  C. difficile  CdtA N-Term and CdtB Receptor Binding Domain Fusion Proteins 
     Expression Plasmid and Recombinant Strain. 
     Genes encoding the fusion protein of CdtA N-term (C49 or C50) with CdtB receptor binding domain protein long or short version (C61 or C62) and a His tag in C-term were cloned into the pET24b(+) expression vector (Novagen) using the Ndel/Xhol restriction sites using standard procedures. The final constructs were generated by the transformation of  E. coli  B834 (DE3) modified strain with the appropriate recombinant expression vector according to standard method with CaCl2-treated cells (Hanahan D. &lt;&lt;Plasmid transformation by Simanis. &gt;&gt; In Glover, D. M. (Ed), DNA cloning. IRL Press London. (1985): p. 109-135.). 
     Fusion CdtA N-Term/CdtB-Receptor Binding Domain 
     C Number 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 C61 
                 CdtA N-term link (aa. 44-268)- 
               
               
                   
                   
                 CdtB RBD short (aa. 636-876) 
               
               
                   
                 C62 
                 CdtA N-term (aa. 44-260)-CdtB 
               
               
                   
                   
                 RBD long (aa. 621-876) 
               
               
                   
                   
               
            
           
         
       
     
     Host Strain 
     Genotype:  E. coli  BL21::DE3 strain, F-ompT hsdS B (r B   − m B   − ) gal dcm (DE3). 
     B834 is the parental strain for BL21. These protease-deficient hosts are methionine auxotrophs. A DE3 lysogens are designed for protein expression from pET vectors This strain is also deficient in the Ion and ompT proteases. 
     Modification: Including PGL gene to avoid phosphogluconoylation in the biotin locus (Strain is auxotroph for biotin). 
     Genotype: B834 ::DE3 strain, F-ompT hsdSB(rB− mB−) gal dcm met (DE3) 
     Modification: Δ(bioA-bioD)::PGL 
     Expression of the Recombinant Proteins: 
       E. coli  transformants were stripped from each agar plate and used to inoculate 200 ml of LBT broth ±1% (w/v) glucose+kanamycin (50 μg/ml) to obtain O.D. 600nm  between 0.1-0.2. Cultures were incubated overnight at 37° C., 250 RPM. 
     These overnight cultures were diluted to 1:20 in 500 ml of LBT medium containing kanamycin (50 μg/ml) and grown at 37° C. at a stirring speed of 250 rpm until O.D. 620  reached 0.5/0.6. 
     At an O.D.at 600 nm of around 0.6, the cultures were cooled down before inducing the expression of the recombinant protein by addition of 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; EMD Chemicals Inc., catalogue number: 5815) and incubated overnight at 23° C., 250 RPM. 
     After the overnight induction (around 16 hours), O.D. at 600 nm was evaluated after induction and culture was centrifuged at 14 000 RPM for 15 minutes and pellets were frozen at −20° C. separately. 
     Purification 
     C61 
     The bacterial pellet was re-suspended in 50 mM bicine buffer (pH 8.0) containing 300 mM NaCl,5 mM TCEP (Thermo Scientific Pierce, (2-carboxyethyl) phosphine hydrochloride), 0.4% empigen and a mixture of protease inhibitors (Complete, Roche). Bacteria were lysed using a French Press system 3×20 000 PSI. Soluble (supernatant) and insoluble (pellet) components were separated by centrifugation at 20 000 g for 30 min at 4° C. 
     The 6-His tagged-protein was purified under native conditions on IMAC. The soluble components were loaded on a 5 ml GE Histrap column (GE) pre-equilibrated with 50 mM bicine buffer (pH 8.0) containing 300 mM NaCl, 0.15% empigen, 1 mM TCEP. After loading on the column, the column was washed with a 50 mM bicine buffer pH8.0, containing 300 mM NaCl, 0.2% tween 20, 25 mM imidazole and 1 mM TCEP. Elution was performed using a 50 mM bicine buffer pH8.0 containing 150 mM NaCl, 0.2% tween 20, 500 mM imidazole and 1 mM TCEP. 
     After desalting step (BIORAD Bio-Gel P6 Desalting) in 50 mM bicine buffer pH8.0 containing 300 mM NaCl, 1 mM TCEP the product was loaded on SEC chromatography (SUPERDEX™ 200) in the same buffer. Fractions containing the recombinant antigen were selected on the basis of purity by SDS-PAGE. Protein concentration was determined using Lowry RC/DC Protein Assay of BioRad. The purified bulk was sterile-filtered on 0.22 μm and stored at −80° C. 
     Example 7 
     Cloning and Expression of  C. difficile  CdtB Mature Co-Expressed (C55) with Prodomain of CdtB C58. 
     Expression Plasmid and Recombinant Strain. 
     Genes encoding protein pro domain of CdtB without His tag was cloned into the pET21b(+) expression vector (Novagen) using the Ndel/Xhol restriction sites using standard procedures. The final construct was generated by the transformation of  E. coli  B834 (DE3) modified strain with the recombinant expression vector of prodomain CdtB and CdtB mature protein C55-information about cloning of C55 see example 3 according to standard method with CaCl2-treated cells (Hanahan D. &lt;&lt;Plasmid transformation by Simanis. &gt;&gt; In Glover, D. M. (Ed), DNA cloning. IRL Press London. (1985): p. 109-135.). 
     Pro Domain of CdtB Alone 
     C Number 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 C58 
                 Pro-domaine CdtB long (aa. 43-211) 
               
               
                   
                   
               
            
           
         
       
     
     Host Strain 
     B834 is the parental strain for BL21. These protease-deficient hosts are methionine auxotrophs. A DE3 lysogens are designed for protein expression from pET vectors This strain is also deficient in the Ion and ompT proteases. 
     Modification: Including PGL gene to avoid phosphogluconoylation in the biotin locus (Strain is auxotroph for biotin). 
     Genotype: B834 ::DE3 strain. F-ompT hsdSB(rB− mB−) gal dcm met (DE3) 
     Modification: Δ(bioA-bioD)::PGL 
     Expression of the Recombinant Proteins: 
     A  E. coli  transformant was stripped from agar plate and used to inoculate 200 ml of LBT broth ±1% (w/v) glucose +kanamycin (50 μg/ml) and ampicillin (100 μg/ml) to obtain O.D. 600nm  between 0.1-0.2. Culture was incubated overnight at 37° C., 250 RPM. 
     This overnight culture was diluted to 1:20 in 500 ml of LBT medium containing kanamycin (50 μg/ml) and ampicillin (100 μg/ml) and grown at 37° C. at a stirring speed of 250 rpm until O.D. 620  reached 0.5/0.6. 
     At an O.D.at 600 nm of around 0.6, the culture was cooled down before inducing the expression of the recombinant protein by addition of 1 mM isopropyl 13-D-1-thiogalactopyranoside (IPTG; EMD Chemicals Inc., catalogue number: 5815) and incubated overnight at 23° C., 250 RPM. 
     After the overnight induction (around 16 hours), O.D. at 600 nm was evaluated after induction and culture was centrifuged at 14 000 RPM for 15 minutes and pellets were frozen at −20° C. separately. 
     Purification 
     same as C55 produced alone 
     Example 8 
     Molecular Weight Evaluation of CdtA, CdtB and CdtA-CdtB Fusion Constructions 
     Analytical ultracentrifugation was used to determine the homogeneity and size distribution in solution of the different species within a protein sample by measuring the rate at which molecules move in response to a centrifugal force. This is based on the calculation of the coefficients of sedimentation of the different species that are obtained by sedimentation velocity experiment, which depend on their molecular shape and mass. 
     1. Protein samples are spun in a Beckman-Coulter ProteomeLab XL-1 analytical ultracentrifuge at 8000 RPM, 25000 RPM or 42000 RPM depending of the target protein size, after the AN-60Ti rotor had been equilibrated to 15° C. 
     2. For data collection, scans were recorded at 280 nm every 5 minutes. 
     3. Data analysis was performed using the program SEDFIT for determination of the C(S) distribution. Determination of the partial specific volume of the proteins was performed with the SEDNTERP software from their amino acid sequence. Sednterp was also used to determine the viscosity and the density of the buffer. 
     4. Determination of the molecular weight of the different species has been determined from the C(S) distribution plot (concentration vs sedimentation coefficient), considering that it&#39;s a better representation of the raw data than the C(M) distribution (concentration vs molecular weight) to characterize the size distribution of a mixture. 
       FIGS. 1 a  to 1 h    describe the size distribution of the different CdtA. CdtB and CdtA-CdtB fusion constructions as determined by sedimentation velocity analytical ultracentrifugation. 
     The calculated molecular weight of the main species for C67 and C69 mutated full length CdtA protein may correspond with a monomer, while the C50 truncated CdtA N-terminal construction is present in solution as a mix of monomer and dimer ( FIGS. 1   a,    1   b  and  1   c ). 
     Both C61 and C62 fusions of CdtA-CdtB are mainly dimeric, with a minor proportion of monomer ( FIGS. 1 d  and 1 e   ). 
     Constructions of the CdtB receptor binding domain C52 and C52 are mainly dimeric with presence of small amount of monomer ( FIGS. 1 f  and 1 g   ). 
     Full length CdtB without prodomain C55 is highly aggregated after purification, presenting a heterogeneous size distribution by AUC ( FIG. 1 h   ). 
     Example 9 
     SDS PAGE Profile of CdtA, CdtB and CdtA-CdtB Fusion Constructions After Purification 
     Purified proteins from each construction were separated on denaturing and reducing SDS PAGE in order to assess the sequence integrity. 
       FIG. 2 a    shows that CdtA-CdtB fusion constructions C61 and C62 are present in majority at the expected molecular weight. Same observations are made for CdtA constructions on  FIG. 2   b.    
     It is shown on  FIG. 2 c    that chymotrypsin activation of C37 CdtB (aa. 43-876) construction results in the truncation of the prodomain for the obtention of a protein (lane 2) at a molecular weight comparable to the mature CdtB represented at the lane 3 by C55 (aa. 212-876). SDS PAGE profile of C55 contains significant amount of secondary products that couln&#39;t be separated from the complete protein, which is coherent with the highly aggregated profile observed by AUC on  FIG. 2   h.    
     CdtB expressed with prodomain C38 (aa. 43-876) was purified as a an heterogeneous preparation composed at the majority of a doublet of the expected molecular weight containing a significant amount of secondary products. 
     Example 9 
     Immunisation of Mice with  C. difficile  CdtA and CdtB Sub-Units Proteins in a ASO1B Formulation 
     Mice Immunisation 
     Groups of 25 female Balb/C mice were immunized IM at days 0, 14 and 28 with 5 μg of full CdtA and CdtB binary toxin purified sub-units. These antigens were injected in an ASO1B formulation. 
     Anti-CdtA and anti-CdtB ELISA titers were determined in individual sera collected at day 42 (Post III 14). Results are shown in  FIGS. 3-4 . 
     A binary toxin cytotoxicity inhibition assay was also performed on pooled Post III sera (day 42). Results are shown in  FIGS. 5-6   
     Anti-CdtA and anti-CdtB ELISA Response: Protocol 
     Full CdtA (C34) or full CdtB (C37)sub-units were coated at 1 μg/ml (for CdtA) or 2 μg/ml (for CdtB) in phosphate buffered saline (PBS) on high-binding microtitre plates (Nunc MAXISORP™), overnight at 4° C. The plates were blocked with PBS-BSA 1% for 30 min at RT with agitation. The mice anti-sera are prediluted 1/500 in PBS-BSA0.2%-TWEEN™ 0.05%. and then, further twofold dilutions were made in microplates and incubated at RT for min. After washing, bound mouse antibody was detected using Jackson ImmunoLaboratories Inc. peroxidase-conjugated Anti-Mouse (ref: 110-035-003) diluted 1:5000 in PBS-BSA0.2%-tween 0.05%. The detection antibodies were incubated for 30 min. at room temperature (RT) with agitation. The color was developed using 4 mg O-phenylenediamine (OPD)+5 μl H 2 O 2  per 10 ml pH 4.5 0.1M citrate buffer for 15 minutes in the dark at room temperature. The reaction was stopped with 50 μl HCl, and the optical density (OD) was read at 490 nm relative to 620 nm. 
     The level of anti-CdtA or anti-CdtB antibodies are expressed in mid-point titers. A GMT was calculated for the 25 samples in each treatment group. 
     Binary Toxin Cytotoxicity Inhibition Assay 
     Human colonic eptithelial cells (HT29 or HCT-116 cells) were cultured at 37° C. with 5% CO 2  in DMEM +10% fetal bovine serum +1% glutamine +1% antibiotics (penicillin-streptomycin-amphotericin) and were seeded in 96-well black tissue culture plates (Greiner Bio-one, Ref: 655090) at a density of 4.10 4  cells/well for HT29 and 1.10 4 cells/well for HCT116. After 24h, the cell media was removed from the wells. 
     The mice anti-sera were prediluted 1:50 in cell media and then, further three-fold dilutions were made in microplate (NUNC, Ref: 163320). 50 μl of serial dilutions of mice pooled antisera were added to the black plates. 50 μl of a mix of CdtA (25 ng/ml) and chemotrypsin-activated CdtB (75 ng/ml) were then added and the black plates incubated at 37° C. with 5% CO 2  for 6 days. 
     After 6 days, the mix of antisera and toxin were removed from the wells and 100 μl of Hoescht stain (BD Pharmingen, Ref: 561908) diluted 1:500 in phosphate buffer saline (PBS) was added in each well for 2 hours in the dark at room temperature. 
     After coloration, the Hoescht stain was removed from the wells and the cells fluorescence cells was measured using an Axiovision microscope. 
     The surface covered by fluorescent staining was determined in each well and cytotoxicity inhibition titers were defined as the reciprocal dilution inducing a 50% inhibition of the fluorescent signal. 
     Example 10 
     Immunisation of Mice with  C. difficile  CdtB Chemotrypsin-Activated or Not, Mixed with F2 or Not, Formulated in AS01B 
     Mice Immunisation 
     Groups of 25 female Balb/C mice were immunized IM at days 0, 14 and 28 with 5 μg of CdtB binary toxin purified sub-unit chemotrypsin-activated or not, mixed with 5 μg of F2 or not. These antigens were injected in an ASO1B formulation. 
     Anti-CdtB, anti-ToxA and anti-ToxB ELISA titers were determined in individual sera collected at day 42 (Post III 14). Results are shown in  FIGS. 7-9 . 
     A binary toxin, ToxA and ToxB cytotoxicity inhibition assay was also performed on pooled Post III sera (day42). Results are shown in  FIGS. 10-12 . 
     Anti-CdtB, Anti-ToxA and Anti-ToxB ELISA Response: Protocol 
     Full CdtB (C37)sub-unit, F2 Cter ToxA and F2 Cter ToxB were coated at 0.5 μg/ml (for CdtB), 2 μg/ml (for ToxA F2 Cter) and 1 μg/ml (for ToxB F2 Cter) in phosphate buffered saline (PBS) on high-binding microtitre plates (Nunc MAXISORP™), overnight at 4° C. The plates were blocked with PBS-BSA 1% for 30 min at RT with agitation. The mice anti-sera are prediluted 1/500 in PBS-BSA0.2%-TWEEN™ 0.05%. and then, further twofold dilutions were made in microplates and incubated at RT for 30 min. After washing, bound mouse antibody was detected using Jackson ImmunoLaboratories Inc. peroxidase-conjugated Anti-Mouse (ref: 110-035-003) diluted 1:5000 in PBS-BSA0.2%-tween 0.05%. The detection antibodies were incubated for 30 min. at room temperature (RT) with agitation. The color was developed using 4 mg O-phenylenediamine (OPD)+5 μl H 2 O 2  per 10 ml pH 4.5 0.1M citrate buffer for 15 minutes in the dark at room temperature. The reaction was stopped with 50 μl HCl, and the optical density (OD) was read at 490 nm relative to 620 nm. 
     The level of anti-CdtB antibodies are expressed in mid-point titers. 
     The level of anti-F2Cter ToxA and F2Cter ToxB antibodies present in each individual sera is determined by comparison to a reference serum added on each plate and expressed in μg/ml. 
     A GMT was calculated for the 25 samples in each treatment group. 
     Binary Toxin, ToxA and ToxB Cytotoxicity Inhibition Assay 
     Human colonic eptithelial cells (HT29 or HCT-116 cells) were cultured at 37° C. with 5%00 2  in DMEM +10% fetal bovine serum +1% glutamine +1% antibiotics (penicillin-streptomycin-amphotericin) and were seeded in 96-well black tissue culture plates (Greiner Bio-one, Ref: 655090) at a density of 4.10 4  cells/well for HT29 and 1.10 4 cells/well for HCT116. After 24h, the cell media was removed from the wells. 
     The mice anti-sera were prediluted 1:5 for g1 (CdtB non-activated) and g2 (CdtB activated) and 1:20 for g3 (CdtB non-activated+F2) and g4 (Cdtb activated+F2) in cell media, for ToxA inhibition cytotoxicity assay, 1:10 for ToxB inhibition cytotoxicity assay and 1:50 for binary toxin inhibition assay. Then, further three-fold dilutions were made in microplate (NUNC, Ref: 163320). 50 μl of serial dilutions of mice pooled antisera were added to the black plates. 50 μl of ToxA (0.01 μg/ml) on HT29, ToxB (0.022 μg/ml) on HCT116 and a mix of CdtA (25 ng/ml) and chemotrypsin-activated CdtB (75 ng/ml) on HT29 and HCT116 were then added in the black plates and incubated at 37° C. with 5% CO 2  for 6 days. 
     After 6 days, the mix of antisera and toxin were removed from the wells and 100p1 of Hoescht stain (BD Pharmingen, Ref: 561908) diluted 1:500 in phosphate buffer saline (PBS) was added in each well for 2 hours in the dark at room temperature. 
     After coloration, the Hoescht stain was removed from the wells and the cells fluorescence cells was measured using an Axiovision microscope. 
     The surface covered by fluorescent staining was determined in each well and cytotoxicity inhibition titers were defined as the reciprocal dilution inducing a 50% inhibition of the fluorescent signal. 
     Example 11 
     Immunisation of Mice with Different Binary Toxin Vaccine Cndidates (CdtA/CdtB) Combined with F2 at 6 μg/Dose in an ASO1B Formulation 
     Mice Immunisation 
     Groups of 20 female Balb/C mice were immunized IM at days 0, 14 and 28 with 6 μg of CdtA-CdtB fusion (C61 and C62), or 3 μg of CdtA (C34, C50 or C67) and/or 3 μg of CdtB (C37, C52, C55 or C55/C58) mixed or not with 6 μg of F2. These antigens were injected in an ASO1 B formulation. 
     Anti-CdtA, anti-CdtB, anti-ToxA and anti-ToxB ELISA titers were determined in individual sera collected at day 42 (Post III 14). Results are shown in  FIGS. 13-16 . 
     A binary toxin, ToxA and ToxB cytotoxicity inhibition assay was also performed on pooled Post III sera (day42). Results are shown in  FIGS. 17-20 . 
     anti-CdtA, anti-CdtB, anti-ToxA F2Cter and anti-ToxB F2 Cter ELISA response: Protocol CdtA mut E428Q (C44), Full CdtB (C37)sub-units, F2 Cter ToxA and F2 Cter ToxB were coated at 1 μg/ml (for CdtA), 0.5 μg/ml (for CdtB), 2 μg/ml (for ToxA F2 Cter) and 1 μg/ml (for ToxB F2 Cter) in phosphate buffered saline (PBS) on high-binding microtitre plates (Nunc MAXISORP™), overnight at 4° C. The plates were blocked with PBS-BSA 1% for 30 min at RT with agitation. The mice anti-sera were prediluted 1:100 (for CdtA, CdtB, ToxB) or 1:200 (for ToxA) for Post II and 1:500 (for CdtA and ToxA), 1:500 or 1:2000 (for CdtB) and 1:250 (for ToxB) for Post III in PBS-BSA0.2%-TWEEN™ 0.05%. Then, further twofold dilutions were made in microplates and incubated at RT for 30 min. After washing, bound mouse antibody was detected using Jackson ImmunoLaboratories Inc. peroxidase-conjugated Anti-Mouse (ref: 110-035-003) diluted 1:5000 in PBS-BSA0.2%-tween 0.05%. The detection antibodies were incubated for 30 min. at room temperature (RT) with agitation. The color was developed using 4 mg O-phenylenediamine (OPD)+5 μl H 2 O 2  per 10 ml pH 4.5 0.1 M citrate buffer for 15 minutes in the dark at room temperature. The reaction was stopped with 50 μl HCl, and the optical density (OD) was read at 490 nm relative to 620 nm. 
     The level of anti-CdtA, anti-CdtB, anti-F2Cter ToxA and F2Cter ToxB antibodies present in each individual sera is determined by comparison to a reference serum added on each plate and expressed in μg/ml. A GMT was calculated for the 20 samples in each treatment group. 
     Binary Toxin, ToxA and ToxB Cytotoxicity Inhibition Assay 
     Human colonic eptithelial cells (HT29 or HCT-116 cells) were cultured at 37° C. with 5%CO 2  in DMEM +10% fetal bovine serum +1% glutamine +1% antibiotics (penicillin-streptomycin-amphotericin) and were seeded in 96-well black tissue culture plates (Greiner Bio-one, Ref: 655090) at a density of 4.10 4  cells/well for HT29 and 1.10 4 cells/well for HCT116. After 24h, the cell media was removed from the wells. 
     The mice anti-sera were prediluted 1:50 in cell media, for ToxA inhibition cytotoxicity assay, 1:10 for ToxB inhibition cytotoxicity assay, 1:50 for binary toxin inhibition assay on HT29 and 1:30 (for Post II) and 1:30 or 1:100 (for Post III) for binary toxin inhibition assay on HCT116. Then, further three-fold dilutions were made in microplate (NUNC, Ref: 163320). 50 μl of serial dilutions of mice pooled antisera were added to the black plates. 50μl of ToxA (0.025 μg/ml) on HT29, ToxB (0.6 μg/ml) on HCT116 and a mix of CdtA (25 ng/ml) and chemotrypsin-activated CdtB (75 ng/ml) on HT29 and HCT116 were then added in the black plates and incubated at 37° C. with 5% CO 2  for 6 days. 
     After 6 days, the mix of antisera and toxin were removed from the wells and 100 μl of Hoescht stain (BD Pharmingen, Ref: 561908) diluted 1:500 in phosphate buffer saline (PBS) was added in each well for 2 hours in the dark at room temperature. 
     After coloration, the Hoescht stain was removed from the wells and the cells fluorescence cells was measured using an Axiovision microscope. 
     The surface covered by fluorescent staining was determined in each well and cytotoxicity inhibition titers were defined as the reciprocal dilution inducing a 50% inhibition of the fluorescent signal. 
     Example 12 
     Immunisation of Mice with Different Binary Toxin Vaccine Candidates (CdtA/CdtB) Combined with F2 at 2 μg/Dose in an ASO1B Formulation 
     Mice Immunisation 
     Groups of 20 female Balb/C mice were immunized IM at days 0, 14 and 28 with 2 μg of CdtA-CdtB fusion (C61 and C62), or 1 μg of CdtA (C34, C50 or C67) and/or 1 μg of CdtB (C37, C52, C55 or C55/C58) mixed or not with 2 μg of F2. These antigens were injected in an ASO1 B formulation. 
     Anti-CdtA, anti-CdtB, anti-ToxA and anti-ToxB ELISA titers were determined in individual sera collected at day 42 (Post III 14). Results are shown in  FIGS. 21-24 . 
     A binary toxin, ToxA and ToxB cytotoxicity inhibition assay was also performed on pooled Post III sera (day42). Results are shown in  FIGS. 25-28 . 
     Anti-CdtA, Anti-CdtB, Anti-ToxA and Anti-ToxB ELISA Response: Protocol 
     CdtA mut E428Q (C44), Full CdtB (C37)sub-units, F2 Cter ToxA and F2 Cter ToxB were coated at 1 μg/ml (for CdtA), 0.5 μg/ml (for CdtB), 2 μg/ml (for ToxA F2 Cter) and 1 μg/ml (for ToxB F2 Cter) in phosphate buffered saline (PBS) on high-binding microtitre plates (Nunc MAXISORP™), overnight at 4° C. The plates were blocked with PBS-BSA 1% for 30 min at RT with agitation. The mice anti-sera were prediluted 1:100 (for CdtB, ToxA,ToxB) and 1:100 or 1:250 (for CdtA) for Post II and 1:500 for Post III in PBS-BSA0.2%-TWEEN™ 0.05%. Then, further twofold dilutions were made in microplates and incubated at RT for 30 min. After washing, bound mouse antibody was detected using Jackson ImmunoLaboratories Inc. peroxidase-conjugated Anti-Mouse (ref: 110-035-003) diluted 1:5000 in PBS-BSA0.2%-tween 0.05%. The detection antibodies were incubated for 30 min. at room temperature (RT) with agitation. The color was developed. 
     Example 13 
     Immunisation of Mice with Different Binary Toxin Vaccine Candidates (CdtA/CdtB) Combined with F2 at 10 μg/Dose in a Non-Adjuvanted Formulation 
     Mice Immunisation 
     Groups of 20 female Balb/C mice were immunized IM at days 0, 14 and 28 with 10 μg of CdtA-CdtB fusion (C61 and C62), or 5 μg of CdtA (C34, C50 or C67) and/or 5 μg of CdtB (C37, C52, C55 or C55/C58) mixed or not with 10 μg of F2. These antigens were injected in a non-adjuvanted formulation. 
     Anti-CdtA, anti-CdtB, anti-ToxA and anti-ToxB ELISA titers were determined in individual sera collected at day 42 (Post III 14). Results are shown in  FIGS. 29-32 . 
     A binary toxin, ToxA and ToxB cytotoxicity inhibition assay was also performed on pooled Post III sera (day42). Results are shown in  FIGS. 33-36 . 
     Anti-CdtA, Anti-CdtB, Anti-ToxA and Anti-ToxB ELISA Response: Protocol 
     CdtA mut E428Q (C44), Full CdtB (C37)sub-units, F2 Cter ToxA and F2 Cter ToxB were coated at 1 μg/ml (for CdtA). 0.5 μg/ml (for CdtB), 2 μg/ml (for F2 Cter ToxA) and 1 μg/ml (for F2 Cter ToxB) in phosphate buffered saline (PBS) on high-binding microtitre plates (Nunc MAXISORP™), overnight at 4° C. The plates were blocked with PBS-BSA 1% for 30 min at RT with agitation. The mice anti-sera were prediluted 1:100 (for CdtA, CdtB, ToxA,ToxB) for Post II and 1:100 (for CdtA, ToxA, ToxB), 1:100 or 1:200 (for CdtB) for Post III in PBS-BSA0.2%-TWEEN™ 0.05%. Then, further twofold dilutions were made in microplates and incubated at RT for 30 min. After washing, bound mouse antibody was detected using Jackson ImmunoLaboratories Inc. peroxidase-conjugated Anti-Mouse (ref: 110-035-003) diluted 1:5000 in PBS-BSA0.2%-tween 0.05%. The detection antibodies were incubated for 30 min. at room temperature (RT) with agitation. The color was developed using 4 mg 0-phenylenediamine (OPD) +5 μl H 2 O 2  per 10 ml pH 4.5 0.1M citrate buffer for 15 minutes in the dark at room temperature. The reaction was stopped with 50 μl HCl, and the optical density (OD) was read at 490 nm relative to 620 nm. 
     A GMT was calculated for the 20 samples in each treatment group. 
     Binary Toxin, ToxA and ToxB Cytotoxicity Inhibition Assay: Protocol 
     Human colonic eptithelial cells (HT29 or HCT-116 cells) were cultured at 37° C. with 5%CO 2  in DMEM +10% fetal bovine serum +1% glutamine +1% antibiotics (penicillin-streptomycin-amphotericin) and were seeded in 96-well black tissue culture plates (Greiner Bio-one, Ref: 655090) at a density of 4.10 4  cells/well for HT29 and 1.10 4 cells/well for HCT116. After 24 h, the cell media was removed from the wells. 
     The mice anti-sera were prediluted 1:50 in cell media, for ToxA inhibition cytotoxicity assay, 1:10 for ToxB inhibition cytotoxicity assay, 1:50 for binary toxin inhibition assay on HT29 and 1:30 (for Post II) and 1:30 or 1:100 (for Post III) for binary toxin inhibition assay on HCT116. Then, further three-fold dilutions were made in microplate (NUNC, Ref: 163320). 
     50 μl of serial dilutions of mice pooled antisera were added to the black plates. 50 μl of ToxA (0.025 μg/ml) on HT29, ToxB (0.6 μg/ml) on HCT116 and a mix of CdtA (25 ng/ml) and chemotrypsin-activated CdtB (75 ng/ml) on HT29 and HCT116 were then added in the black plates and incubated at 37° C. with 5% CO 2  for 6 days. 
     After 6 days, the mix of antisera and toxin were removed from the wells and 100 μl of Hoescht stain (BD Pharmingen, Ref: 561908) diluted 1:500 in phosphate buffer saline (PBS) was added in each well for 2 hours in the dark at room temperature. 
     After coloration, the Hoescht stain was removed from the wells and the cells fluorescence cells was measured using an Axiovision microscope. 
     The surface covered by fluorescent staining was determined in each well and cytotoxicity inhibition titers were defined as the reciprocal dilution inducing a 50% inhibition of the fluorescent signal. 
     Example 14 
     Cloning and Expression of  C. difficile  F2 and CdtB Receptor Binding Domain Fusion Proteins 
     Expression Plasmid and Recombinant Strain. 
     Genes encoding the fusion protein of F2 protein with CdtB receptor binding domain protein long or short version (C64 and C65) and a His tag in C-term were cloned into the pET24b(+) expression vector (Novagen) using the Ndel/Xhol restriction sites using standard procedures. 
     Fusion F2/CdtB—Receptor Binding Domain 
     C Number 
     
       
         
           
               
               
               
             
               
                   
                   
               
             
            
               
                   
                 C64 
                 CdtA N-term (aa. 44-260)-CdtB RBD long (aa. 
               
               
                   
                   
                 621-876) 
               
               
                   
                 C65 
                 F2-CdtB RBD short (aa. 636-876) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE A 
               
             
            
               
                   
               
               
                 Sequence Summary 
               
            
           
           
               
               
               
               
            
               
                   
                 Construct 
                 Amino acid 
                 Polynucleotide 
               
               
                 Description 
                 reference 
                 sequence 
                 sequence 
               
               
                   
               
               
                 CDTa full length (strain R20291) 
                 N/A 
                 SEQ.I.D.NO: 1 
                 SEQ.I.D.NO: 2 
               
               
                 CDTb full length (strain R20291) 
                 N/A 
                 SEQ.I.D.NO: 3 
                 SEQ.I.D.NO: 4 
               
               
                 CDTa without signal peptide 
                 C34 
                 SEQ.I.D.NO: 5 
                 SEQ.I.D.NO: 6 
               
               
                 CDTb′ (minus signal peptide) 
                 C37 
                 SEQ.I.D.NO: 7 
                 SEQ.I.D.NO: 8 
               
               
                 ligated to Glutathione-S-transferase 
               
               
                 protein.(GST underlined) 
               
               
                 CDTb″ (minus pro-domain and 
                 C40 
                 SEQ.I.D.NO: 9 
                 N/A 
               
               
                 signal peptide) 
               
               
                 CDTa mutation E4280 
                 C44 
                 SEQ.I.D.NO: 10 
                 SEQ.I.D.NO: 11 
               
               
                 CDTa mutation E430Q 
                 C54 
                 SEQ.I.D.NO: 12 
                 N/A 
               
               
                 CDTa N terminal domain (residue 
                 Gulke et 
                 SEQ.I.D.NO: 13 
                 N/A 
               
               
                 44 to residue 240) 
                 al 2001 
               
               
                 CDTa without signal peptide, with a 
                 C49 
                 SEQ.I.D.NO: 14 
                 N/A 
               
               
                 linker between the N-term domain 
               
               
                 and the C-term domain (containing 
               
               
                 the enzymatic activity). This 
               
               
                 construct covers the fragment from 
               
               
                 amino acid 44 to aa 268. 
               
               
                 CDTa without signal peptide or 
                 C50 
                 SEQ.I.D.NO: 15 
               
               
                 linker. This construct covers the 
               
               
                 fragment from aa 44 to aa 260. 
               
               
                 CDTb minus signal peptide (CDTb′) 
                 C38 
                 SEQ ID NO: 16 
                 SEQ ID NO: 17 
               
               
                 Fusion 1 
                 F1 
                 SEQ ID NO: 18 
               
               
                 Fusion 2 
                 F2 
                 SEQ ID NO: 19 
               
               
                 Fusion 3 
                 F3 
                 SEQ ID NO: 20 
               
               
                 Fusion 4 
                 F4 
                 SEQ ID NO: 21 
               
               
                 Fusion 5 
                 F5 
                 SEQ ID NO: 22 
               
               
                 Fusion F54 Gly 
                 N/A 
                 SEQ ID NO: 24 
                 SEQ ID NO: 23 
               
               
                 Fusion F54 New 
                 N/A 
                 SEQ ID NO: 26 
                 SEQ ID NO: 25 
               
               
                 Fusion F5 ToxB 
                 N/A 
                 SEQ ID NO: 28 
                 SEQ ID NO: 27 
               
               
                 Fusion F52 New 
                 N/A 
                 SEQ ID NO: 30 
                 SEQ ID NO: 29 
               
               
                 Toxin A 
                 N/A 
                 SEQ ID NO: 31 
               
               
                 Toxin B 
                 N/A 
                 SEQ ID NO: 32 
               
               
                 CDTb″ (minus pro-domain and 
                 C39 
                 SEQ ID NO: 33 
                 N/A 
               
               
                 signal peptide) ligated to 
               
               
                 Glutathione-S-transferase protein. 
               
               
                 CdtB receptor binding domain with 
                 C52 
                 SEQ ID NO: 34 
                 SEQ ID NO: 35 
               
               
                 linker in N-term of sequence, from 
               
               
                 aa 620-876 
               
               
                 CdtB receptor binding domain 
                 C53 
                 SEQ ID NO: 36 
                 SEQ ID NO: 37 
               
               
                 without linker in N-term of 
               
               
                 sequence, from aa 636-876 
               
               
                 CDTb with prodomain removed 
                 C55 
                 SEQ ID NO: 51 
               
               
                 (CDTb″, aa212-876) 
               
               
                 CDTb prodomain sequence (long, 
                 C58 
                 SEQ ID NO: 38 
                 N/A 
               
               
                 aa43-211) 
               
               
                 CDTb prodomain sequence (short, 
                 C59 
                 SEQ ID NO: 39 
                 N/A 
               
               
                 aa43-186) 
               
               
                 Fusion CDTa N-term with linker 
                 C60 
                 SEQ ID NO: 40 
                 N/A 
               
               
                 (aa44-268) to CDTb receptor 
               
               
                 binding domain with linker in N term 
               
               
                 of sequence (aa621-876) 
               
               
                 Fusion CDTa N-term with linker 
                 C61 
                 SEQ ID NO: 41 
                 N/A 
               
               
                 (aa44-268) to CDTb receptor 
               
               
                 binding domain without linker in N 
               
               
                 term of sequence (aa636-876) 
               
               
                 Fusion CDTa N-term without linker 
                 C62 
                 SEQ ID NO: 42 
                 N/A 
               
               
                 (aa44-260) to CDTb receptor 
               
               
                 binding domain with linker in N term 
               
               
                 of sequence (aa621-876) 
               
               
                 Fusion CDTa N-term without linker 
                 C63 
                 SEQ ID NO: 43 
                 N/A 
               
               
                 (aa44-260) to CDTb receptor 
               
               
                 binding domain without linker in N 
               
               
                 term of sequence (aa636-876) 
               
               
                 Fusion F2-CDTb receptor binding 
                 C64 
                 SEQ ID NO: 44 
                 N/A 
               
               
                 domain with linker in N term of 
               
               
                 sequence (aa621-876) 
               
               
                 Fusion of F2 to CDTb receptor 
                 C65 
                 SEQ ID NO: 45 
                 N/A 
               
               
                 binding domain without linker in N 
               
               
                 term of sequence (aa636-876) with 
               
               
                 2 heterogeneous Gly residues 
               
               
                 between F2 and CTDb sequences 
               
               
                 CDTa without signal peptide, with 
                 C67 
                 SEQ ID NO: 46 
                 SEQ ID NO: 47 
               
               
                 two mutations (E428Q, E430Q, aa 
               
               
                 44-463). 
               
               
                 CDTa without signal peptide, with 
                 C69 
                 SEQ ID NO: 48 
                 SEQ ID NO: 49 
               
               
                 seven mutations (R345A, Q350A, 
               
               
                 N385A, R402A, S388F, E428Q, 
               
               
                 E430Q, aa 44-463). 
               
               
                 CDTb without signal sequence and 
                 C97 
                 SEQ ID NO: 50 
                 N/A 
               
               
                 prodomain (mature fragment based 
               
               
                 on MS data) with Ca2+ binding 
               
               
                 motif mutation (aa212-876, mut 
               
               
                 Asp-9-11-13 Ala) 
               
               
                 CDTa without signal peptide, with 
                 C107 
                 SEQ ID NO: 52 
                 SEQ ID NO: 53 
               
               
                 five mutations (R345A, Q350A, 
               
               
                 N385A, R402A, S388F, aa 44-463). 
               
               
                 CDTa without signal peptide, with 
                 C108 
                 SEQ ID NO: 54 
                 SEQ ID NO: 55 
               
               
                 six mutations (R345A, Q350A, 
               
               
                 N385A, R402A, S388F, E430Q, aa 
               
               
                 44-463). 
               
               
                 CdtA without signal peptide, with six 
                 C110 
                 SEQ ID NO: 56 
                 N/A 
               
               
                 mutations (R345A-Q350A-N385A- 
               
               
                 R402A-S388F-E428Q, aa 44-463). 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                   
               
               
                 SEQUENCE LISTING 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 SEQ ID 1-CDTa full length polypeptide sequence  
               
               
                 MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKVCNTTYKAPIERPEDFLKDKE  
               
               
                 KAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQTRNYFYDYQIEANSREKEYKEL 
               
               
                 RNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLEKENEFKETIQNKLFKQDGEKDIS  
               
               
                 LYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAE  
               
               
                 ASVVSSLDFKDDVSKGDSWGKANYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPV  
               
               
                 NNPNPELDSKITNIENALKREPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWE  
               
               
                 GQALSYPNFISTSIGSVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKI 
               
               
                 NKIDSYKDGTITKLIVDATLIP 
               
               
                   
               
               
                 SEQ ID 2-CDTa full length polynucleotide sequence  
               
               
                 ATGAAAAAATTTAGGAAACATAAAAGGATTAGTAATTGTATATCTATATTGTTGATATTAT 
               
               
                 ATCTAACTTTAGGTGGTTTGTTACCTAATAACATTTATGCACAAGACTTACAAAGCTATA  
               
               
                 GTGAAAAAGTTTGCAATACTACTTACAAGGCTCCTATAGAAAGACCAGAAGATTTTCTTA  
               
               
                 AAGATAAAGAAAAGGCTAAAGAATGGGAAAGAAAAGAAGCAGAAAGAATAGAGCAAAAA  
               
               
                 CTTGAAAGATCTGAAAAAGAAGCATTAGAATCATATAAAAAAGATTCTGTAGAAATAAGT 
               
               
                 AAATATTCTCAGACAAGAAATTATTTTTATGATTATCAAATAGAAGCAAATTCTCGAGAAA  
               
               
                 AAGAATATAAAGAACTTCGAAATGCTATATCAAAAAATAAAATAGATAAACCTATGTATGT 
               
               
                 CTATTATTTTGAATCTCCAGAAAAATTTGCATTTAATAAAGTAATAAGAACAGAAAATCAA  
               
               
                 AACGAAATTTCATTAGAAAAATTTAATGAGTTTAAAGAAACTATACAAAACAAATTATTTA  
               
               
                 AGCAAGATGGATTTAAAGATATTTCTTTATATGAACCTGGAAAAGGTGATGAAAAACCTA  
               
               
                 CACCATTACTTATGCACTTAAAATTACCTAGAAATACTGGTATGTTACCATATACAAATAC  
               
               
                 TAACAATGTAAGTACATTAATAGAGCAAGGATATAGTATAAAAATAGATAAAATTGTTCGT 
               
               
                 ATAGTTATAGATGGGAAGCACTATATTAAAGCAGAAGCATCTGTTGTAAGTAGTCTTGAT 
               
               
                 TTTAAAGATGATGTAAGTAAGGGGGATTCTTGGGGTAAAGCAAATTATAATGATTGGAG 
               
               
                 TAATAAATTAACACCTAATGAACTTGCTGATGTAAATGATTATATGCGTGGAGGATATAC  
               
               
                 TGCAATTAATAATTATTTAATATCAAATGGTCCAGTAAATAATCCTAACCCAGAATTAGAT 
               
               
                 TCTAAAATCACAAACATTGAAAATGCATTAAAACGTGAACCTATTCCAACTAATTTAACTG 
               
               
                 TATATAGAAGATCTGGTCCTCAAGAATTTGGTTTAACTCTTACTTCCCCTGAATATGATTT 
               
               
                 TAACAAACTAGAAAATATAGATGCTTTTAAATCAAAATGGGAAGGACAAGCACTGTCTTA  
               
               
                 TCCAAACTTTATTAGTACTAGTATTGGTAGTGTGAATATGAGTGCATTTGCTAAAAGAAA  
               
               
                 AATAGTACTACGTATAACTATACCTAAAGGTTCTCCTGGAGCTTATCTATCAGCTATTCC  
               
               
                 AGGTTATGCAGGTGAATATGAAGTGCTTTTAAATCATGGAAGCAAATTTAAAATCAATAA  
               
               
                 AATTGATTCTTACAAAGATGGTACTATAACAAAATTAATTGTTGATGCAACATTGATACCT 
               
               
                 TAA  
               
               
                   
               
               
                 SEQ ID 3-CDTb full length polypeptide sequence  
               
               
                 MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEIVNEDILPNNGLMGYYFTDE  
               
               
                 HFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRWTGRIIPSKDGEYTLSTDRDDVLM  
               
               
                 QVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGSIDNLSSPNLYWELDGMKKIIPEENLFLR  
               
               
                 DYSNIEKDDPFIPNNNFFDPKLMSDWEDEDLDTDNDNIPDSYERNGYTIKDLIAVKWEDSFA  
               
               
                 EQGYKKYVSNYLESNTAGDPYTDYEKASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTN 
               
               
                 EHASTDQGKTVSRATTNSKTESNTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNG 
               
               
                 ESWNTGLSINKGESAYINANVRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLS  
               
               
                 PGDTYPKKGLSPLALNTMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQI 
               
               
                 VTEGNSWSDYISQIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKK 
               
               
                 DGLLYFNDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDDY  
               
               
                 NNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSNSIIVKI 
               
               
                 KAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITELNSTPEILDEPEV  
               
               
                 KIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEALDYIQKYRVEATLQYSGFK 
               
               
                 DIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYKKLRIYAITPDDRELLVLSVD  
               
               
                   
               
               
                 SEQ ID 4-CDTb full length polynucleotide sequence  
               
               
                 ATGAAAATACAAATGAGGAATAAAAAGGTATTAAGTTTTTTAACACTTACAGCTATAGTTA  
               
               
                 GTCAAGCACTAGTATATCCTGTATATGCTCAAACTAGTACAAGTAATCATTCTAATAAGA  
               
               
                 AAAAAGAAATTGTAAATGAAGATATACTCCCAAACAATGGATTAATGGGATATTATTTCA  
               
               
                 CAGATGAGCACTTTAAAGATTTAAAATTAATGGCACCCATAAAAGATGGTAATTTAAAAT 
               
               
                 TTGAAGAAAAGAAAGTAGATAAACTTCTGGATAAAGACAAATCAGATGTAAAATCTATAC  
               
               
                 GATGGACAGGAAGAATAATTCCTTCTAAGGATGGTGAATATACATTATCAACTGATAGA  
               
               
                 GATGATGTCTTAATGCAAGTAAATACTGAGAGTACTATATCAAATACACTTAAAGTTAATA  
               
               
                 TGAAAAAGGGTAAAGAATATAAAGTTAGAATAGAGCTACAAGATAAAAATTTAGGTTCAA  
               
               
                 TAGATAATTTATCATCACCTAATCTTTATTGGGAATTAGATGGTATGAAGAAAATTATACC  
               
               
                 AGAAGAAAATTTATTCTTAAGAGATTATTCTAATATAGAAAAAGATGATCCATTTATCCCA  
               
               
                 AATAACAATTTCTTTGACCCAAAGTTGATGTCTGATTGGGAAGACGAAGATTTGGATACA  
               
               
                 GATAATGATAATATACCAGATTCATATGAACGAAATGGATATACTATTAAGGACTTAATT 
               
               
                 GCAGTTAAGTGGGAAGATAGTTTTGCAGAACAAGGCTATAAGAAATATGTATCAAATTAT 
               
               
                 TTAGAGTCAAATACTGCTGGAGATCCATATACAGATTATGAAAAAGCTTCAGGTTCTTTT 
               
               
                 GACAAGGCTATAAAGACTGAAGCAAGAGATCCGTTAGTTGCAGCATATCCAATTGTTGG 
               
               
                 AGTAGGTATGGAAAAATTAATTATATCTACAAATGAACATGCCTCTACTGATCAAGGTAA  
               
               
                 AACTGTTTCCAGAGCTACTACTAACAGTAAAACTGAATCTAATACAGCTGGTGTGTCTGT 
               
               
                 TAATGTAGGATATCAAAATGGATTCACAGCTAATGTAACTACAAATTATTCCCATACAAC  
               
               
                 AGATAATTCAACTGCTGTTCAAGATAGTAATGGAGAATCATGGAATACTGGATTAAGTAT 
               
               
                 AAACAAAGGAGAATCTGCATATATAAATGCAAATGTTAGATATTACAACACAGGTACTGC  
               
               
                 ACCTATGTACAAAGTGACACCAACAACAAATTTAGTGTTAGATGGAGATACATTATCAAC  
               
               
                 TATCAAAGCACAAGAAAATCAAATTGGCAATAATCTATCTCCTGGAGATACTTATCCCAA  
               
               
                 AAAAGGGCTTTCACCTCTAGCTCTTAACACAATGGATCAATTTAGCTCTAGACTGATTCC  
               
               
                 TATAAATTATGATCAATTAAAAAAATTAGATGCTGGAAAGCAAATTAAATTAGAAACAACA  
               
               
                 CAAGTAAGTGGAAATTTTGGTACAAAAAATAGTTCTGGACAAATAGTAACAGAAGGAAAT 
               
               
                 AGTTGGTCAGACTATATAAGTCAAATTGACAGTATTTCTGCATCTATTATATTAGATACAG 
               
               
                 AGAATGAATCTTACGAAAGAAGAGTTACTGCTAAAAATTTACAGGATCCAGAAGATAAAA  
               
               
                 CACCTGAACTTACAATTGGAGAAGCAATTGAAAAAGCTTTTGGCGCTACTAAAAAAGAT 
               
               
                 GGTTTGTTATATTTTAATGATATACCAATAGATGAAAGTTGTGTTGAACTCATATTTGATG 
               
               
                 ATAATACAGCCAATAAGATTAAAGATAGTTTAAAAACTTTGTCTGATAAAAAGATATATAA  
               
               
                 TGTTAAACTTGAAAGAGGAATGAATATACTTATAAAAACACCAACTTACTTTACTAATTTT 
               
               
                 GATGATTATAATAATTACCCTAGTACATGGAGTAATGTCAATACTACGAATCAAGATGGT 
               
               
                 TTACAAGGCTCAGCAAATAAATTAAATGGTGAGACGAAGATTAAAATCCCTATGTCTGAG 
               
               
                 CTAAAACCTTATAAACGTTATGTTTTTAGTGGATATTCAAAGGATCCTTTAACATCTAATT 
               
               
                 CAATAATTGTAAAGATAAAAGCAAAAGAAGAGAAAACGGATTATTTGGTACCAGAACAA  
               
               
                 GGATATACAAAATTTAGTTATGAATTTGAAACTACTGAAAAAGATTCTTCTAATATAGAGA  
               
               
                 TAACATTAATTGGTAGTGGTACAACATACTTAGATAACTTATCTATTACAGAGCTAAATAG 
               
               
                 TACTCCTGAAATACTTGATGAACCAGAAGTTAAAATTCCAACTGACCAAGAAATAATGGA  
               
               
                 TGCACATAAAATATATTTTGCAGATTTAAATTTTAATCCAAGTACAGGAAATACTTATATA  
               
               
                 AATGGTATGTATTTTGCACCAACACAAACTAATAAAGAAGCTCTCGATTATATCCAAAAA  
               
               
                 TATAGAGTTGAAGCTACTTTACAATATTCTGGATTTAAAGATATTGGAACTAAAGATAAA  
               
               
                 GAAATGCGTAATTATTTAGGAGATCCAAATCAGCCTAAAACTAATTATGTTAATCTTAGG 
               
               
                 AGTTATTTTACAGGTGGAGAAAATATTATGACATACAAGAAATTAAGAATATATGCAATTA  
               
               
                 CTCCAGACGATAGAGAGTTATTAGTTCTTAGTGTTGATTAG 
               
               
                   
               
               
                 SEQ ID 5-CDTa C34 construct polypeptide sequence  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
               
               
                 TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLE  
               
               
                 KFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIE  
               
               
                 QGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKANYNDWSNKLTPNELAD  
               
               
                 VNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKREPIPTNLTVYRRSGPQEFGLT 
               
               
                 LTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIGSVNMSAFAKRKIVLRITIPKGSPGAYL 
               
               
                 SAIPGYAGEYEVLLNHGSKFKINKIDSYKDGTITKLIVDATLIP 
               
               
                   
               
               
                 SEQ ID 6-CDTb C34 construct polynucleotide sequence  
               
               
                 ATGGTTTGCAATACCACCTATAAAGCACCGATTGAACGTCCGGAAGATTTTCTGAAAGA  
               
               
                 TAAAGAAAAAGCCAAAGAATGGGAACGCAAAGAAGCAGAACGTATTGAACAGAAACTG 
               
               
                 GAACGTAGCGAAAAAGAAGCACTGGAAAGCTACAAAAAAGATAGCGTGGAAATTTCAAA  
               
               
                 ATATAGCCAGACCCGCAATTATTTCTATGATTATCAGATTGAAGCCAATAGCCGTGAAAA  
               
               
                 AGAATATAAAGAACTGCGCAATGCCATTAGCAAAAACAAAATTGATAAACCGATGTATGT 
               
               
                 GTATTATTTCGAAAGTCCGGAAAAATTTGCCTTTAACAAAGTGATTCGCACCGAAAATCA  
               
               
                 GAATGAAATTAGCCTGGAAAAATTCAATGAATTTAAAGAAACCATTCAGAATAAACTGTT 
               
               
                 TAAACAGGATGGCTTTAAAGATATTTCACTGTATGAACCGGGTAAAGGTGATGAAAAAC  
               
               
                 CGACACCGCTGCTGATGCATCTGAAACTGCCTCGTAATACCGGTATGCTGCCGTATAC  
               
               
                 CAATACCAATAATGTTAGCACCCTGATTGAACAGGGCTATAGCATCAAAATTGATAAAAT 
               
               
                 TGTGCGCATTGTGATTGATGGCAAACATTATATCAAAGCCGAAGCCAGCGTTGTTTCAA  
               
               
                 GCCTGGATTTTAAAGATGATGTGAGCAAAGGCGATAGCTGGGGTAAAGCAAACTATAAT 
               
               
                 GATTGGAGCAATAAACTGACCCCGAATGAACTGGCAGATGTGAATGATTATATGCGTGG 
               
               
                 TGGTTATACCGCCATTAACAATTATCTGATTAGCAATGGTCCGGTGAATAATCCGAATCC  
               
               
                 GGAACTGGATAGCAAAATTACCAATATTGAAAATGCCCTGAAACGCGAACCGATTCCGA  
               
               
                 CCAATCTGACCGTTTATCGTCGTAGCGGTCCGCAAGAATTTGGTCTGACCCTGACCAGT 
               
               
                 CCGGAATATGACTTTAACAAACTGGAAAATATTGATGCCTTTAAAAGCAAATGGGAAGG 
               
               
                 TCAGGCACTGAGCTATCCGAACTTTATTAGCACCAGCATTGGTAGCGTTAATATGAGCG 
               
               
                 CATTTGCCAAACGTAAAATTGTGCTGCGTATTACCATTCCGAAAGGTAGTCCGGGTGCA  
               
               
                 TATCTGAGCGCAATTCCGGGTTATGCCGGTGAATATGAAGTTCTGCTGAATCATGGCAG 
               
               
                 CAAATTCAAAATTAACAAAATTGATAGCTATAAAGATGGCACCATTACCAAACTGATTGT 
               
               
                 TGATGCAACCCTGATTCCGTAA  
               
               
                   
               
               
                 SEQ ID 7-CDTb C37 construct. CDTb′ (minus signal peptide) ligated to Glutathione-  
               
               
                 S-transferase protein (GST underlined) polypeptide sequence.  
               
               
                 
                   MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGD  
                 
               
               
                 
                   VKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKL 
                 
               
               
                 
                   PEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIP 
                 
               
               
                   QIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGS HMEIVNEDILPNNGLMG 
               
               
                 YYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRWTGRIIPSKDGEYTLSTDR  
               
               
                 DDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGSIDNLSSPNLYWELDGMKKIIPE  
               
               
                 ENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDEDLDTDNDNIPDSYERNGYTIKDLIAVK 
               
               
                 WEDSFAEQGYKKYVSNYLESNTAGDPYTDYEKASGSFDKAIKTEARDPLVAAYPIVGVGME  
               
               
                 KLIISTNEHASTDQGKTVSRATTNSKTESNTAGVSVNVGYQNGFTANVTTNYSHTTDNSTA  
               
               
                 VQDSNGESWNTGLSINKGESAYINANVRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQEN 
               
               
                 QIGNNLSPGDTYPKKGLSPLALNTMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGT 
               
               
                 KNSSGQIVTEGNSWSDYISQIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEK 
               
               
                 AFGATKKDGLLYFNDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTY  
               
               
                 FTNFDDYNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPL 
               
               
                 TSNSIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITELNSTP 
               
               
                 EILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEALDYIQKYRVEA  
               
               
                 TLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYKKLRIYAITPDDREL 
               
               
                 LVLSVD  
               
               
                   
               
               
                 SEQ ID 8-CDTb C37construct. CDTb′ (minus pro-domain) ligated to Glutathione-S-  
               
               
                 transferase protein (GST underlined) polynucleotide sequence.  
               
               
                 
                   atatcccctatactaggtttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaag  
                 
               
               
                 
                   agcatttgtatgagcgcgatgaagatgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatatt  
                 
               
               
                 
                   gatggtgatgttaaattaacacagtctatggccatcatacgttatatacactgacaagcacaacatgttgggtggttgtccaaaagag  
                 
               
               
                 
                   cgtgcagagatttcaatgcttgaacnagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactct  
                 
               
               
                 
                   caaagttgattttcttagcaagctacctgaaatgctgaaaatcttccoagatcgtttatgtcataaaacatatttaaatggtgatcatgt  
                 
               
               
                 
                   aacccatcctgacttcatgttatataacactcttgatcatcatttatacatggacccaatctgcctgclatacgttcccaaaattagttt  
                 
               
               
                 
                   cttttaaaaaacgtattaaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaa  
                 
               
               
                   gccacgtttgatggtgccgaccatcctccaaaatcggatctggaagttctgttccaggggcccctgggatcc catatggaaattgtg  
               
               
                 aatgaagatattctgccgaataatggtctgatgggatactactttaccgatgaacattttaaagatctgaaactgatggcaccgatta  
               
               
                 aagatggcaatctgaaatttgaagaaaaaaaagtggataaactgctggataaagataaaagtgatgtgaaaagcattcgttgg  
               
               
                 accggtcgtattattccgagcaaagatggtgaatacaccctgagcaccgatcgtgatgatgttctgatgcaggttaataccgaaag  
               
               
                 caccattagcaataccctgaaagtgaatatgaaaaaaggcaaagaatataaagtgcgcattgaactgcaggataaaaatctgg  
               
               
                 gtagcattgataatctgagcagcccgaatctgtattgggaactggatggtatgaaaaaaatcattccggaagaaaacctgtttctg  
               
               
                 cgcgattatagcaatattgaaaaagatgatccgtttattccgaataataacttttttgatccgaaactgatgagcgattgggaagatg  
               
               
                 aagatctggataccgataatgataatattccggatagctatgaacgcaatggctataccattaaagatctgattgccgtgaaatgg  
               
               
                 gaagatagctttgcagaacagggctataagaaatatgtgagcaattatctggaaagcaataccgcaggcgatccgtataccgat  
               
               
                 tatgaaaaagcaagcggcagctttgataaagccattaaaaccgaagcacgtgatccgctggttgcagcatatccgattgttggtg  
               
               
                 ttggtatggaaaaactgattattagcaccaatgaacatgcaagcaccgatcagggtaaaaccgttagccgtgcaaccaccaata  
               
               
                 gcaaaaccgaaagcaatacagccggtgttagcgttaatgttggttatcagaatggttttaccgccaatgtgaccaccaattatagc  
               
               
                 cataccaccgataatagcaccgcagttcaggatagcaatggtgaaagctggaataccggtctgagcattaacaaaggtgaaa  
               
               
                 gcgcatatatcaatgccaatgtgcgctattataacaccggcaccgcaccgatgtataaagttaccccgaccaccaatctggttctg  
               
               
                 gatggtgataccctgagtaccattaaagcacaagaaaatcagattggcaataatctgagtccgggtgatacctatccgaaaaaa  
               
               
                 ggtctgagtccgctggcactgaataccatggatcagtttagcagccgtctgattccgattaactatgatcagctgaaaaaactggat  
               
               
                 gccggtaaacaaatcaaactggaaaccacccaggttagcggtaattttggcaccaaaaattcaagcggtcagattgttaccgaa  
               
               
                 ggtaatagctggtcagattatatcagccagattgatagcattagcgccagcattattctggatacagaaaatgaaagctatgaacg  
               
               
                 tcgtgtgaccgcaaaaaatctgcaggacccggaagataaaacaccggaactgaccattggtgaagcaattgaaaaagcattt  
               
               
                 ggtgccaccaaaaaagatggcctgctgtattttaacgatattccgattgatgaaagctgcgtggaactgatttttgatgataataccg  
               
               
                 ccaataaaatcaaagatagcctgaaaaccctgagcgacaaaaaaatctataatgtgaaactggaacgcggtatgaatattctg  
               
               
                 attaaaaccccgacctattttaccaattttgatgattataacaattatccgagcacttggagcaatgtgaataccaccaatcaggatg  
               
               
                 gtctgcagggtagcgcaaataaactgaatggtgaaaccaaaatcaaaattccgatgagcgaactgaaaccgtataaacgttat  
               
               
                 gtgtttagcggctatagcaaagatccgctgaccagcaatagcattattgtgaaaatcaaagccaaagaagaaaaaaccgattat  
               
               
                 ctggttccggaacagggttataccaaatttagctatgaatttgaaaccaccgaaaaagatagcagtaatattgaaattaccctgatt  
               
               
                 ggtagcggcaccacctatctggataatctgagtattaccgaactgaatagcacaccggaaattctggatgaaccggaagtgaaa  
               
               
                 attccgaccgatcaagaaattatggatgcccataaaatctattttgccgatctgaactttaatccgagcaccggcaatacctatatta  
               
               
                 acggcatgtattttgcaccgacccagaccaataaagaagccctggattatattcagaaatatcgtgttgaagccaccctgcagtat  
               
               
                 agcggttttaaagatattggcaccaaagataaagaaatgcgtaattatctgggcgatccgaatcagccgaaaaccaattatgtta  
               
               
                 atctgcgcagctattttaccggtggcgaaaacattatgacctacaaaaaactgcgcatttatgccattacaccggatgatcgtgaa  
               
               
                 ctgctggttctgagcgttgattaa  
               
               
                   
               
               
                 SEQ ID 9-CDTb C40 construct. CDTb″ (minus pro-domain and signal peptide)  
               
               
                 polypeptide sequence.  
               
               
                 LMSDWEDEDLDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDP 
               
               
                 YTDYEKASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTE  
               
               
                 SNTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINANV  
               
               
                 RYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALNTMDQ  
               
               
                 FSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYISQIDSISASIIL 
               
               
                 DTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYFNDIPIDESCVELIFDDN 
               
               
                 TANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDDYNNYPSTWSNVNTTNQDGLQG 
               
               
                 SANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKFSY  
               
               
                 EFETTEKDSSNIEITLIGSGTTYLDNLSITELNSTPElLDEPEVKIPTDQEIMDAHKIYFADLNFN 
               
               
                 PSTGNTY1NGMYFAPTQTNKEALDYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPK 
               
               
                 TNYVNLRSYFTGGENIMTYKKLRIYAITPDDRELLVLSVD  
               
               
                   
               
               
                 SEQ ID 10-C44 construct. CDTa mutation E428Q polypeptide sequence.  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
               
               
                 TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLE  
               
               
                 KFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIE  
               
               
                 QGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKANYNDWSNKLTPNELAD  
               
               
                 VNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKREPIPTNLTVYRRSGPQEFGLT 
               
               
                 LTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIGSVNMSAFAKRKIVLRITIPKGSPGAYL 
               
               
                 SAIPGYAGqYEVLLNHGSKFKINKIDSYKDGTITKLIVDATLIP 
               
               
                   
               
               
                 SEQ ID 11-CDTa mutation E428Q polynucleotide sequence.  
               
               
                 atggtttgcaataccacctataaagcaccgattgaacgtccggaagattttctgaaagataaagaaaaagccaaagaatggga  
               
               
                 acgcaaagaagcagaacgtattgaacagaaactggaacgtagcgaaaaagaagcactggaaagctacaaaaaagatagc  
               
               
                 gtggaaatttcaaaatatagccagacccgcaattatttctatgattatcagattgaagccaatagccgtgaaaaagaatataaaga  
               
               
                 actgcgcaatgccattagcaaaaacaaaattgataaaccgatgtatgtgtattatttcgaaagtccggaaaaatttgcctttaacaa  
               
               
                 agtgattcgcaccgaaaatcagaatgaaattagcctggaaaaattcaatgaatttaaagaaaccattcagaataaactgtttaaa  
               
               
                 caggatggctttaaagatatttcactgtatgaaccgggtaaaggtgatgaaaaaccgacaccgctgctgatgcatctgaaactgc  
               
               
                 ctcgtaataccggtatgctgccgtataccaataccaataatgttagcaccctgattgaacagggctatagcatcaaaattgataaa  
               
               
                 attgtgcgcattgtgattgatggcaaacattatatcaaagccgaagccagcgttgtttcaagcctggattttaaagatgatgtgagca  
               
               
                 aaggcgatagctggggtaaagcaaactataatgattggagcaataaactgaccccgaatgaactggcagatgtgaatgattat  
               
               
                 atgcgtggtggttataccgccattaacaattatctgattagcaatggtccggtgaataatccgaatccggaactggatagcaaaatt  
               
               
                 accaatattgaaaatgccctgaaacgcgaaccgattccgaccaatctgaccgtttatcgtcgtagcggtccgcaagaatttggtct  
               
               
                 gaccctgaccagtccggaatatgactttaacaaactggaaaatattgatgcctttaaaagcaaatgggaaggtcaggcactgag  
               
               
                 ctatccgaactttattagcaccagcattggtagcgttaatatgagcgcatttgccaaacgtaaaattgtgctgcgtattaccattccga  
               
               
                 aaggtagtccgggtgcatatctgagcgcaattccgggttatgccggtCaatatgaagttctgctgaatcatggcagcaaattcaaa  
               
               
                 attaacaaaattgatagctataaagatggcaccattaccaaactgattgttgatgcaaccctgattccgtaa  
               
               
                   
               
               
                 SEQ ID 12-C54 construct. CDTa mutation E430Q polypeptide sequence.  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
               
               
                 TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLE  
               
               
                 KFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIE  
               
               
                 QGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKANYNDWSNKLTPNELAD  
               
               
                 VNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKREPIPTNLTVYRRSGPQEFGLT 
               
               
                 LTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIGSVNMSAFAKRKIVLRITIPKGSPGAYL 
               
               
                 SAIPGYAGEYqVLLNHGSKFKINKIDSYKDGTITKLIVDATLIP 
               
               
                   
               
               
                 SEQ ID 13-CDTa N terminal domain (residue 44 to residue 240) polypeptide  
               
               
                 sequence.  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
               
               
                 TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLE  
               
               
                 KFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIE  
               
               
                 QGYSIKIDKI 
               
               
                   
               
               
                 SEQ ID 14-C49 construct. CDTa Nterminal domain without signal peptide, with the  
               
               
                 linker existing between the N-term domain and the C-term domain (containing the  
               
               
                 enzymatic activity). This construct covers the fragment from amino acid 44 to aa 268  
               
               
                 polypeptide sequence.  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
               
               
                 TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLE  
               
               
                 KFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIE  
               
               
                 QGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVS  
               
               
                   
               
               
                 SEQ ID 15-C50 construct. CDTa without signal peptide and the linker existing  
               
               
                 between the Nterminal and Cterminal domains of CDTa. This construct covers the  
               
               
                 fragment from aa 44 to aa 260 polypeptide sequence.  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
               
               
                 TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLE  
               
               
                 KFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIE  
               
               
                 QGYSIKIDKIVRIVIDGKHYIKAEASVVSS  
               
               
                   
               
               
                 SEQ ID NO: 16-Polypeptide sequence of CDTb with pro-domain removed (CDTb′)  
               
               
                 EIVNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRWTG 
               
               
                 RIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGSIDNLSSP 
               
               
                 NLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDEDLDTDNDNIPDS  
               
               
                 YERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEKASGSFDKAIKTEAR  
               
               
                 DPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTESNTAGVSVNVGYQNGFTA  
               
               
                 NVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINANVRYYNTGTAPMYKVTPTTNL 
               
               
                 VLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALNTMDQFSSRLIPINYDQLKKLDAGK 
               
               
                 QIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYISQ1DSISASIILDTENESYERRVTAKNLQD  
               
               
                 PEDKTPELTIGEAIEKAFGATKKDGLLYFNDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYN 
               
               
                 VKLERGMNILIKTPTYFTNFDDYNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELK 
               
               
                 PYKRYVFSGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGS  
               
               
                 GTTYLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQ  
               
               
                 TNKEALDYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIM  
               
               
                 TYKKLRIYAITPDDRELLVLSVD  
               
               
                   
               
               
                 SEQ ID NO: 17-Polypeptide sequence of CDTb with pro-domain removed (CDTb′)  
               
               
                 catatggaaattgtgaatgaagatattctgccgaataatggtctgatgggatactactttaccgatgaacattttaaagatctgaaac  
               
               
                 tgatggcaccgattaaagatggcaatctgaaatttgaagaaaaaaaagtggataaactgctggataaagataaaagtgatgtg  
               
               
                 aaaagcattcgttggaccggtcgtattattccgagcaaagatggtgaatacaccctgagcaccgatcgtgatgatgttctgatgca  
               
               
                 ggttaataccgaaagcaccattagcaataccctgaaagtgaatatgaaaaaaggcaaagaatataaagtgcgcattgaactgc  
               
               
                 aggataaaaatctgggtagcattgataatctgagcagcccgaatctgtattgggaactggatggtatgaaaaaaatcattccgga  
               
               
                 agaaaacctgtttctgcgcgattatagcaatattgaaaaagatgatccgtttattccgaataataacttttttgatccgaaactgatga  
               
               
                 gcgattgggaagatgaagatctggataccgataatgataatattccggatagctatgaacgcaatggctataccattaaagatctg  
               
               
                 attgccgtgaaatgggaagatagctttgcagaacagggctataagaaatatgtgagcaattatctggaaagcaataccgcaggc  
               
               
                 gatccgtataccgattatgaaaaagcaagcggcagctttgataaagccattaaaaccgaagcacgtgatccgctggttgcagca  
               
               
                 tatccgattgttggtgttggtatggaaaaactgattattagcaccaatgaacatgcaagcaccgatcagggtaaaaccgttagccg  
               
               
                 tgcaaccaccaatagcaaaaccgaaagcaatacagccggtgttagcgttaatgttggttatcagaatggttttaccgccaatgtga  
               
               
                 ccaccaattatagccataccaccgataatagcaccgcagttcaggatagcaatggtgaaagctggaataccggtctgagcatta  
               
               
                 acaaaggtgaaagcgcatatatcaatgccaatgtgcgctattataacaccggcaccgcaccgatgtataaagttaccccgacca  
               
               
                 ccaatctggttctggatggtgataccctgagtaccattaaagcacaagaaaatcagattggcaataatctgagtccgggtgatacc  
               
               
                 tatccgaaaaaaggtctgagtccgctggcactgaataccatggatcagtttagcagccgtctgattccgattaactatgatcagctg  
               
               
                 aaaaaactggatgccggtaaacaaatcaaactggaaaccacccaggttagcggtaattttggcaccaaaaattcaagcggtca  
               
               
                 gattgttaccgaaggtaatagctggtcagattatatcagccagattgatagcattagcgccagcattattctggatacagaaaatga  
               
               
                 aagctatgaacgtcgtgtgaccgcaaaaaatctgcaggacccggaagataaaacaccggaactgaccattggtgaagcaatt  
               
               
                 gaaaaagcatttggtgccaccaaaaaagatggcctgctgtatataacgatattccgattgatgaaagctgcgtggaactgatttttg  
               
               
                 atgataataccgccaataaaatcaaagatagcctgaaaaccctgagcgacaaaaaaatctataatgtgaaactggaacgcgg  
               
               
                 tatgaatattctgattaaaaccccgacctattttaccaattttgatgattataacaattatccgagcacttggagcaatgtgaataccac  
               
               
                 caatcaggatggtctgcagggtagcgcaaataaactgaatggtgaaaccaaaatcaaaattccgatgagcgaactgaaaccg  
               
               
                 tataaacgttatgtgtttagcggctatagcaaagatccgctgaccagcaatagcattattgtgaaaatcaaagccaaagaagaaa  
               
               
                 aaaccgattatctggttccggaacagggttataccaaatttagctatgaatttgaaaccaccgaaaaagatagcagtaatattgaa  
               
               
                 attaccctgattggtagcggcaccacctatctggataatctgagtattaccgaactgaatagcacaccggaaattctggatgaacc  
               
               
                 ggaagtgaaaattccgaccgatcaagaaattatggatgcccataaaatctattttgccgatctgaactttaatccgagcaccggca  
               
               
                 atacctatattaacggcatgtattttgcaccgacccagaccaataaagaagccctggattatattcagaaatatcgtgttgaagcca  
               
               
                 ccctgcagtatagcggttttaaagatattggcaccaaagataaagaaatgcgtaattatctgggcgatccgaatcagccgaaaa  
               
               
                 ccaattatgttaatctgcgcagctattttaccggtggcgaaaacattatgacctacaaaaaactgcgcatttatgccattacaccgg  
               
               
                 atgatcgtgaactgctggttctgagcgttgattaa  
               
               
                   
               
               
                 SEQ ID NO: 18-sequence of Fusion 1 (F1)  
               
               
                 MGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDAN 
               
               
                 NIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYF 
               
               
                 SYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNK 
               
               
                 FLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAAT 
               
               
                 GWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANN 
               
               
                 IEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYY  
               
               
                 FNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRF 
               
               
                 LYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVF 
               
               
                 KGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYFM  
               
               
                 PDTAMAAAGGLFEIDGVIYFFGVDGVKAPGFVSINDNKHYFDDSGVMKVGYTEIDGKHFYF 
               
               
                 AENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTAVVGWK 
               
               
                 DLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFSDSGIIESGVQN 
               
               
                 IDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGETY  
               
               
                 TIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNEN 
               
               
                 GEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDLD  
               
               
                 EKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVISE  
               
               
                   
               
               
                 SEQ ID NO: 19-sequence of Fusion 2 (F2)  
               
               
                 MGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDAN 
               
               
                 NIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGINRIINNKKYYFNPNNAIAAIHLCTINNDKYYF 
               
               
                 SYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNK 
               
               
                 FLTLNGKKYYFDNDSKAVTGWQT1DGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAAT 
               
               
                 GWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANN 
               
               
                 IEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYY  
               
               
                 FNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRF 
               
               
                 LYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVF 
               
               
                 KGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGINQTINGKVYYFM  
               
               
                 PDTAMAAAGGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKHFY  
               
               
                 FAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTAVVGW  
               
               
                 KDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFSDSGIIESGVQ  
               
               
                 NIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGET 
               
               
                 YTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNE  
               
               
                 NGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDL 
               
               
                 DEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVISE  
               
               
                   
               
               
                 SEQ ID NO: 20-sequence of Fusion 3 (F3)  
               
               
                 MGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMU GVFKGPNGFEYFAPANTDAN 
               
               
                 NIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYF 
               
               
                 SYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNK 
               
               
                 FLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAAT 
               
               
                 GWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANN 
               
               
                 IEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYY  
               
               
                 FNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRF 
               
               
                 LYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVF 
               
               
                 KGSNGFEYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFD  
               
               
                 EDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDN 
               
               
                 GIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDME  
               
               
                 NESDKYYFNPETKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIE  
               
               
                 DKMFYFGEDGVMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYI  
               
               
                 AATGSVIIDGEEYYFDPDTAQLVISE  
               
               
                   
               
               
                 SEQ ID NO: 21-sequence of Fusion 4 (F4)  
               
               
                 MGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDAN 
               
               
                 NIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYF 
               
               
                 SYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNK 
               
               
                 FLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAAT 
               
               
                 GWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANN 
               
               
                 IEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYY  
               
               
                 FNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRF 
               
               
                 LYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVF 
               
               
                 KGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYFM  
               
               
                 PDTAMAAAGGETIIDDKNYYFNQSGVLQTGVFSTEDGFKYFAPANTLDENLEGEAIDFTGKL 
               
               
                 IIDENIYYFDDNYRGAVEWKELDGEMHYFSPETGKAFKGLNQIGDYKYYFNSDGVMQKGFV  
               
               
                 SINDNKHYFDDSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEE  
               
               
                 GEEISYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFND  
               
               
                 DGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANT 
               
               
                 VNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLI 
               
               
                 DDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPD  
               
               
                 GFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQL 
               
               
                 VISE  
               
               
                   
               
               
                 SEQ ID NO: 22-sequence of Fusion 5 (F5)  
               
               
                 MGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDAN 
               
               
                 NIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYF 
               
               
                 SYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNK 
               
               
                 FLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEAAT 
               
               
                 GWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANN 
               
               
                 IEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYY  
               
               
                 FNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRF 
               
               
                 LYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVF 
               
               
                 KGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYFM  
               
               
                 PDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYGGGFVSINDNKHYFDDSGVMKVGYTEIDG 
               
               
                 KHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTAV  
               
               
                 VGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFSDSGIIE  
               
               
                 SGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGEDVYY  
               
               
                 FGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNY  
               
               
                 YFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTG 
               
               
                 WLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVISE  
               
               
                   
               
               
                 SEQ ID NO: 23-nucleotide sequence of F54 Gly  
               
               
                 ATGGCAACCGGTTGGCAGACCATCGATGGCAAAAAATATTATTTTAATACCAACACCGC  
               
               
                 AATTGCAAGCACCGGCTATACCATTATCAACGGCAAACACTTTTATTTTAACACCGACG 
               
               
                 GCATTATGCAGATTGGTGTGTTTAAAGGTCCGAACGGCTTTGAATACTTTGCACCGGCA  
               
               
                 AATACCGATGCCAATAATATTGAAGGCCAGGCCATTCTGTATCAGAATGAATTTCTGAC  
               
               
                 CCTGAACGGCAAAAAATACTACTTTGGCAGCGATAGCAAAGCAGTTACCGGTTGGCGC  
               
               
                 ATCATCAACAATAAGAAATATTACTTCAACCCGAATAATGCAATTGCAGCAATTCATCTG 
               
               
                 TGCACCATTAACAACGACAAATATTATTTCAGCTATGACGGTATTCTGCAGAATGGCTAC  
               
               
                 ATTACCATCGAACGCAACAACTTTTATTTCGATGCCAACAACGAAAGCAAAATGGTGAC  
               
               
                 CGGTGTTTTCAAAGGCCCTAATGGTTTTGAGTATTTCGCTCCGGCAAACACCCATAATA  
               
               
                 ACAACATTGAAGGTCAGGCGATCGTTTATCAGAACAAATTCCTGACGCTGAATGGTAAG 
               
               
                 AAATACTATTTCGATAATGACAGCAAAGCCGTGACCGGCTGGCAGACAATTGACGGGA  
               
               
                 AGAAATATTACTTTAATCTGAATACCGCAGAAGCAGCAACCGGTTGGCAAACGATCGAC  
               
               
                 GGTAAAAAGTACTACTTCAACCTGAACACAGCCGAAGCAGCCACAGGATGGCAGACTA  
               
               
                 TTGATGGAAAAAAATACTATTTCAACACCAACACCTTTATTGCATCTACCGGTTATACCA  
               
               
                 GCATTAACGGTAAACATTTCTACTTCAACACCGATGGTATCATGCAGATCGGCGTTTTCA  
               
               
                 AAGGTCCAAATGGTTTCGAATACTTTGCCCCTGCCAATACAGATGCAAATAACATCGAG 
               
               
                 GGTCAGGCAATCCTGTACCAAAACAAATTTCTGACCCTGAATGGGAAAAAATATTACTTT 
               
               
                 GGTAGCGATTCTAAAGCCGTTACCGGTCTGCGTACCATTGATGGTAAAAAATACTACTT 
               
               
                 TAATACGAATACAGCCGTTGCGGTTACAGGCTGGCAGACCATTAACGGGAAAAAATACT 
               
               
                 ATTTTAACACAAATACCAGCATTGCCTCAACGGGTTATACCATTATTTCGGGTAAACACT 
               
               
                 TCTACTTTAATACCGATGGTATTATGCAAATCGGAGTCTTTAAAGGACCTGATGGGTTCG 
               
               
                 AATATTTTGCGCCTGCGAACACTGATGCGAACAATATCGAAGGACAGGCAATCCGCTAT 
               
               
                 CAGAATCGCTTTCTGTATCTGCACGACAACATCTATTATTTTGGCAACAATTCAAAAGCA  
               
               
                 GCCACCGGCTGGGTTACAATTGATGGCAACCGCTACTATTTCGAACCGAATACCGCAAT 
               
               
                 GGGTGCAAATGGCTACAAAACCATCGATAATAAAAATTTCTATTTTCGCAACGGTCTGC  
               
               
                 CGCAGATCGGGGTATTTAAAGGTAGCAACGGCTTCGAATACTTCGCTCCAGCGAATAC  
               
               
                 GGACGCGAACAATATTGAGGGTCAAGCGATTCGTTATCAAAACCGTTTTCTGCATCTGC  
               
               
                 TGGGCAAAATCTACTACTTTGGCAATAACAGTAAAGCAGTTACTGGATGGCAGACAATC  
               
               
                 AATGGTAAAGTGTACTATTTTATGCCGGATACCGCCATGGCAGCAGCCGGTGGTCTGTT 
               
               
                 TGAAATTGATGGCGTGATCTATTTTTTTGGTGTGGATGGTGTTAAAGCACCGGGAATAT 
               
               
                 ACGGTGGTACCGGCTTTGTGACCGTGGGTGATGATAAATACTATTTCAATCCGATTAAC  
               
               
                 GGTGGTGCAGCGAGCATTGGCGAAACCATCATCGATGACAAAAACTATTATTTCAACCA  
               
               
                 GAGCGGTGTGCTGCAGACCGGTGTGTTTAGCACCGAAGATGGCTTTAAATATTTTGCG 
               
               
                 CCAGCGAACACCCTGGATGAAAACCTGGAAGGCGAAGCGATTGATTTTACCGGCAAAC  
               
               
                 TGATCATCGATGAAAACATCTATTACTTCGATGATAACTATCGTGGTGCGGTGGAATGG 
               
               
                 AAAGAACTGGATGGCGAAATGCATTATTTTTCTCCGGAAACCGGTAAAGCGTTTAAAGG 
               
               
                 CCTGAACCAGATCGGCGATTACAAATACTACTTCAACAGCGATGGCGTGATGCAGAAA  
               
               
                 GGCTTTGTGAGCATCAACGATAACAAACACTATTTCGATGATAGCGGTGTGATGAAAGT 
               
               
                 GGGCTATACCGAAATTGATGGCAAACATTTCTACTTCGCGGAAAACGGCGAAATGCAGA  
               
               
                 TTGGCGTGTTCAATACCGAAGATGGTTTCAAATACTTCGCGCACCATAACGAAGATCTG 
               
               
                 GGTAACGAAGAAGGCGAAGAAATTAGCTATAGCGGCATCCTGAACTTCAACAACAAAAT 
               
               
                 CTACTACTTTGATGATAGCTTTACCGCGGTGGTGGGCTGGAAAGATCTGGAAGATGGC  
               
               
                 AGCAAATATTATTTCGATGAAGATACCGCGGAAGCGTATATTGGCCTGAGCCTGATTAA  
               
               
                 CGATGGCCAGTACTATTTTAACGATGATGGCATTATGCAGGTGGGTTTCGTGACCATTA  
               
               
                 ATGATAAAGTGTTCTATTTCAGCGATAGCGGCATTATTGAAAGCGGCGTGCAGAACATT 
               
               
                 GATGATAACTACTTCTACATCGATGATAACGGCATTGTGCAGATCGGCGTTTTTGATAC  
               
               
                 CAGCGATGGCTACAAATATTTCGCACCGGCCAATACCGTGAACGATAACATTTATGGCC  
               
               
                 AGGCGGTGGAATATAGCGGTCTGGTGCGTGTGGGCGAAGATGTGTATTATTTCGGCGA  
               
               
                 AACCTATACCATCGAAACCGGCTGGATTTATGATATGGAAAACGAAAGCGATAAATATTA  
               
               
                 CTTTAATCCGGAAACGAAAAAAGCGTGCAAAGGCATTAACCTGATCGATGATATCAAAT 
               
               
                 ACTATTTTGATGAAAAAGGCATTATGCGTACCGGTCTGATTAGCTTCGAAAACAACAACT 
               
               
                 ATTACTTCAACGAAAACGGTGAAATGCAGTTCGGCTACATCAACATCGAAGATAAAATG 
               
               
                 TTCTACTTCGGCGAAGATGGTGTTATGCAGATTGGTGTTTTTAACACCCCGGATGGCTT 
               
               
                 CAAATACTTTGCCCATCAGAATACCCTGGATGAAAATTTCGAAGGTGAAAGCATTAACTA  
               
               
                 TACCGGCTGGCTGGATCTGGATGAAAAACGCTACTACTTCACCGATGAATACATTGCGG 
               
               
                 CGACCGGCAGCGTGATTATTGATGGCGAAGAATACTACTTCGATCCGGATACCGCGCA  
               
               
                 GCTGGTGATTAGCGAACATCATCATCATCACCAT 
               
               
                   
               
               
                 SEQ ID NO: 24-amino acid of F54Gly  
               
               
                 MATGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTD  
               
               
                 ANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKY  
               
               
                 YFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQ  
               
               
                 NKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYY  
               
               
                 FNLNTAEAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEY  
               
               
                 FAPANTDANNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTG 
               
               
                 WQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIE  
               
               
                 GQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKT 
               
               
                 IDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNS  
               
               
                 KAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYGGTGFVTVGDDK 
               
               
                 YYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFSTEDGFKYFAPANTLDENLEGEAIDFT 
               
               
                 GKLIIDENIYYFDDNYRGAVEWKELDGEMHYFSPETGKAFKGLNQIGDYKYYFNSDGVMQK 
               
               
                 GFVSINDNKHYFDDSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLG 
               
               
                 NEEGEEISYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYY  
               
               
                 FNDDGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAP 
               
               
                 ANTVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKG 
               
               
                 INLIDDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFN 
               
               
                 TPDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDT 
               
               
                 AQLVISEHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 25-nucleotide sequence of F54 New  
               
               
                 ATGGCAACCGGTTGGCAGACCATCGATGGCAAAAAATATTATTTTAATACCAACACCGC  
               
               
                 AATTGCAAGCACCGGCTATACCATTATCAACGGCAAACACTTTTATTTTAACACCGACG 
               
               
                 GCATTATGCAGATTGGTGTGTTTAAAGGTCCGAACGGCTTTGAATACTTTGCACCGGCA  
               
               
                 AATACCGATGCCAATAATATTGAAGGCCAGGCCATTCTGTATCAGAATGAATTTCTGAC  
               
               
                 CCTGAACGGCAAAAAATACTACTTTGGCAGCGATAGCAAAGCAGTTACCGGTTGGCGC  
               
               
                 ATCATCAACAATAAGAAATATTACTTCAACCCGAATAATGCAATTGCAGCAATTCATCTG 
               
               
                 TGCACCATTAACAACGACAAATATTATTTCAGCTATGACGGTATTCTGCAGAATGGCTAC  
               
               
                 ATTACCATCGAACGCAACAACTTTTATTTCGATGCCAACAACGAAAGCAAAATGGTGAC  
               
               
                 CGGTGTTTTCAAAGGCCCTAATGGTTTTGAGTATTTCGCTCCGGCAAACACCCATAATA  
               
               
                 ACAACATTGAAGGTCAGGCGATCGTTTATCAGAACAAATTCCTGACGCTGAATGGTAAG 
               
               
                 AAATACTATTTCGATAATGACAGCAAAGCCGTGACCGGCTGGCAGACAATTGACGGGA  
               
               
                 AGAAATATTACTTTAATCTGAATACCGCAGAAGCAGCAACCGGTTGGCAAACGATCGAC  
               
               
                 GGTAAAAAGTACTACTTCAACCTGAACACAGCCGAAGCAGCCACAGGATGGCAGACTA  
               
               
                 TTGATGGAAAAAAATACTATTTCAACACCAACACCTTTATTGCATCTACCGGTTATACCA  
               
               
                 GCATTAACGGTAAACATTTCTACTTCAACACCGATGGTATCATGCAGATCGGCGTTTTCA  
               
               
                 AAGGTCCAAATGGTTTCGAATACTTTGCCCCTGCCAATACAGATGCAAATAACATCGAG 
               
               
                 GGTCAGGCAATCCTGTACCAAAACAAATTTCTGACCCTGAATGGGAAAAAATATTACTTT 
               
               
                 GGTAGCGATTCTAAAGCCGTTACCGGTCTGCGTACCATTGATGGTAAAAAATACTACTT 
               
               
                 TAATACGAATACAGCCGTTGCGGTTACAGGCTGGCAGACCATTAACGGGAAAAAATACT 
               
               
                 ATTTTAACACAAATACCAGCATTGCCTCAACGGGTTATACCATTATTTCGGGTAAACACT 
               
               
                 TCTACTTTAATACCGATGGTATTATGCAAATCGGAGTCTTTAAAGGACCTGATGGGTTCG 
               
               
                 AATATTTTGCGCCTGCGAACACTGATGCGAACAATATCGAAGGACAGGCAATCCGCTAT 
               
               
                 CAGAATCGCTTTCTGTATCTGCACGACAACATCTATTATTTTGGCAACAATTCAAAAGCA  
               
               
                 GCCACCGGCTGGGTTACAATTGATGGCAACCGCTACTATTTCGAACCGAATACCGCAAT 
               
               
                 GGGTGCAAATGGCTACAAAACCATCGATAATAAAAATTTCTATTTTCGCAACGGTCTGC  
               
               
                 CGCAGATCGGGGTATTTAAAGGTAGCAACGGCTTCGAATACTTCGCTCCAGCGAATAC  
               
               
                 GGACGCGAACAATATTGAGGGTCAAGCGATTCGTTATCAAAACCGTTTTCTGCATCTGC  
               
               
                 TGGGCAAAATCTACTACTTTGGCAATAACAGTAAAGCAGTTACTGGATGGCAGACAATC  
               
               
                 AATGGTAAAGTGTACTATTTTATGCCGGATACCGCCATGGCAGCAGCCGGTGGTCTGTT 
               
               
                 TGAAATTGATGGCGTGATCTATTTTTTTGGTGTGGATGGTGTTAAAGCAGTTACCGGCTT 
               
               
                 TGTGACCGTGGGTGATGATAAATACTATTTCAATCCGATTAACGGTGGTGCAGCGAGCA  
               
               
                 TTGGCGAAACCATCATCGATGACAAAAACTATTATTTCAACCAGAGCGGTGTGCTGCAG 
               
               
                 ACCGGTGTGTTTAGCACCGAAGATGGCTTTAAATATTTTGCGCCAGCGAACACCCTGGA  
               
               
                 TGAAAACCTGGAAGGCGAAGCGATTGATTTTACCGGCAAACTGATCATCGATGAAAACA  
               
               
                 TCTATTACTTCGATGATAACTATCGTGGTGCGGTGGAATGGAAAGAACTGGATGGCGAA  
               
               
                 ATGCATTATTTTTCTCCGGAAACCGGTAAAGCGTTTAAAGGCCTGAACCAGATCGGCGA  
               
               
                 TTACAAATACTACTTCAACAGCGATGGCGTGATGCAGAAAGGCTTTGTGAGCATCAACG 
               
               
                 ATAACAAACACTATTTCGATGATAGCGGTGTGATGAAAGTGGGCTATACCGAAATTGAT 
               
               
                 GGCAAACATTTCTACTTCGCGGAAAACGGCGAAATGCAGATTGGCGTGTTCAATACCGA  
               
               
                 AGATGGTTTCAAATACTTCGCGCACCATAACGAAGATCTGGGTAACGAAGAAGGCGAA  
               
               
                 GAAATTAGCTATAGCGGCATCCTGAACTTCAACAACAAAATCTACTACTTTGATGATAGC  
               
               
                 TTTACCGCGGTGGTGGGCTGGAAAGATCTGGAAGATGGCAGCAAATATTATTTCGATGA  
               
               
                 AGATACCGCGGAAGCGTATATTGGCCTGAGCCTGATTAACGATGGCCAGTACTATTTTA  
               
               
                 ACGATGATGGCATTATGCAGGTGGGTTTCGTGACCATTAATGATAAAGTGTTCTATTTCA  
               
               
                 GCGATAGCGGCATTATTGAAAGCGGCGTGCAGAACATTGATGATAACTACTTCTACATC  
               
               
                 GATGATAACGGCATTGTGCAGATCGGCGTTTTTGATACCAGCGATGGCTACAAATATTT 
               
               
                 CGCACCGGCCAATACCGTGAACGATAACATTTATGGCCAGGCGGTGGAATATAGCGGT 
               
               
                 CTGGTGCGTGTGGGCGAAGATGTGTATTATTTCGGCGAAACCTATACCATCGAAACCG 
               
               
                 GCTGGATTTATGATATGGAAAACGAAAGCGATAAATATTACTTTAATCCGGAAACGAAAA  
               
               
                 AAGCGTGCAAAGGCATTAACCTGATCGATGATATCAAATACTATTTTGATGAAAAAGGCA  
               
               
                 TTATGCGTACCGGTCTGATTAGCTTCGAAAACAACAACTATTACTTCAACGAAAACGGT 
               
               
                 GAAATGCAGTTCGGCTACATCAACATCGAAGATAAAATGTTCTACTTCGGCGAAGATGG 
               
               
                 TGTTATGCAGATTGGTGTTTTTAACACCCCGGATGGCTTCAAATACTTTGCCCATCAGAA  
               
               
                 TACCCTGGATGAAAATTTCGAAGGTGAAAGCATTAACTATACCGGCTGGCTGGATCTGG 
               
               
                 ATGAAAAACGCTACTACTTCACCGATGAATACATTGCGGCGACCGGCAGCGTGATTATT 
               
               
                 GATGGCGAAGAATACTACTTCGATCCGGATACCGCGCAGCTGGTGATTAGCGAACATC  
               
               
                 ATCATCATCACCAT 
               
               
                   
               
               
                 SEQ ID NO: 26 amino acid sequence of F54 New  
               
               
                 MATGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTD  
               
               
                 ANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKY  
               
               
                 YFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQ  
               
               
                 NKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEA  
               
               
                 ATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDA  
               
               
                 NNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKK 
               
               
                 YYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQN 
               
               
                 RFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIG 
               
               
                 VFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYF 
               
               
                 MPDTAMAAAGGLFEIDGVIYFFGVDGVKAVTGFVTVGDDKYYFNPINGGAASIGETIIDDKN 
               
               
                 YYFNQSGVLQTGVFSTEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVE  
               
               
                 WKELDGEMHYFSPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMK 
               
               
                 VGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYY  
               
               
                 FDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFY  
               
               
                 FSDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVR  
               
               
                 VGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIMIKYYFDEKGIMRTGLI 
               
               
                 SFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFAHQNTLDENFE  
               
               
                 GESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVISEHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 27 nucleotide sequence of F5 ToxB  
               
               
                 ATGGCAACCGGTTGGCAGACCATCGATGGCAAAAAATATTATTTTAATACCAACACCGC  
               
               
                 AATTGCAAGCACCGGCTATACCATTATCAACGGCAAACACTTTTATTTTAACACCGACG 
               
               
                 GCATTATGCAGATTGGTGTGTTTAAAGGTCCGAACGGCTTTGAATACTTTGCACCGGCA  
               
               
                 AATACCGATGCCAATAATATTGAAGGCCAGGCCATTCTGTATCAGAATGAATTTCTGAC  
               
               
                 CCTGAACGGCAAAAAATACTACTTTGGCAGCGATAGCAAAGCAGTTACCGGTTGGCGC  
               
               
                 ATCATCAACAATAAGAAATATTACTTCAACCCGAATAATGCAATTGCAGCAATTCATCTG 
               
               
                 TGCACCATTAACAACGACAAATATTATTTCAGCTATGACGGTATTCTGCAGAATGGCTAC  
               
               
                 ATTACCATCGAACGCAACAACTTTTATTTCGATGCCAACAACGAAAGCAAAATGGTGAC  
               
               
                 CGGTGTTTTCAAAGGCCCTAATGGTTTTGAGTATTTCGCTCCGGCAAACACCCATAATA  
               
               
                 ACAACATTGAAGGTCAGGCGATCGTTTATCAGAACAAATTCCTGACGCTGAATGGTAAG 
               
               
                 AAATACTATTTCGATAATGACAGCAAAGCCGTGACCGGCTGGCAGACAATTGACGGGA  
               
               
                 AGAAATATTACTTTAATCTGAATACCGCAGAAGCAGCAACCGGTTGGCAAACGATCGAC  
               
               
                 GGTAAAAAGTACTACTTCAACCTGAACACAGCCGAAGCAGCCACAGGATGGCAGACTA  
               
               
                 TTGATGGAAAAAAATACTATTTCAACACCAACACCTTTATTGCATCTACCGGTTATACCA  
               
               
                 GCATTAACGGTAAACATTTCTACTTCAACACCGATGGTATCATGCAGATCGGCGTTTTCA  
               
               
                 AAGGTCCAAATGGTTTCGAATACTTTGCCCCTGCCAATACAGATGCAAATAACATCGAG 
               
               
                 GGTCAGGCAATCCTGTACCAAAACAAATTTCTGACCCTGAATGGGAAAAAATATTACTTT 
               
               
                 GGTAGCGATTCTAAAGCCGTTACCGGTCTGCGTACCATTGATGGTAAAAAATACTACTT 
               
               
                 TAATACGAATACAGCCGTTGCGGTTACAGGCTGGCAGACCATTAACGGGAAAAAATACT 
               
               
                 ATTTTAACACAAATACCAGCATTGCCTCAACGGGTTATACCATTATTTCGGGTAAACACT 
               
               
                 TCTACTTTAATACCGATGGTATTATGCAAATCGGAGTCTTTAAAGGACCTGATGGGTTCG 
               
               
                 AATATTTTGCGCCTGCGAACACTGATGCGAACAATATCGAAGGACAGGCAATCCGCTAT 
               
               
                 CAGAATCGCTTTCTGTATCTGCACGACAACATCTATTATTTTGGCAACAATTCAAAAGCA  
               
               
                 GCCACCGGCTGGGTTACAATTGATGGCAACCGCTACTATTTCGAACCGAATACCGCAAT 
               
               
                 GGGTGCAAATGGCTACAAAACCATCGATAATAAAAATTTCTATTTTCGCAACGGTCTGC  
               
               
                 CGCAGATCGGGGTATTTAAAGGTAGCAACGGCTTCGAATACTTCGCTCCAGCGAATAC  
               
               
                 GGACGCGAACAATATTGAGGGTCAAGCGATTCGTTATCAAAACCGTTTTCTGCATCTGC  
               
               
                 TGGGCAAAATCTACTACTTTGGCAATAACAGTAAAGCAGTTACTGGATGGCAGACAATC  
               
               
                 AATGGTAAAGTGTACTATTTTATGCCGGATACCGCCATGGCAGCAGCCGGTGGTCTGTT 
               
               
                 TGAAATTGATGGCGTGATCTATTTTTTTGGTGTGGATGGTGTTAAAGCAGTGAGCGGTC  
               
               
                 TGATTTATATTAACGATAGCCTGTATTACTTTAAACCACCGGTGAATAACCTGATTACCG 
               
               
                 GCTTTGTGACCGTGGGTGATGATAAATACTATTTCAATCCGATTAACGGTGGTGCAGCG 
               
               
                 AGCATTGGCGAAACCATCATCGATGACAAAAACTATTATTTCAACCAGAGCGGTGTGCT 
               
               
                 GCAGACCGGTGTGTTTAGCACCGAAGATGGCTTTAAATATTTTGCGCCAGCGAACACC  
               
               
                 CTGGATGAAAACCTGGAAGGCGAAGCGATTGATTTTACCGGCAAACTGATCATCGATGA  
               
               
                 AAACATCTATTACTTCGATGATAACTATCGTGGTGCGGTGGAATGGAAAGAACTGGATG 
               
               
                 GCGAAATGCATTATTTTTCTCCGGAAACCGGTAAAGCGTTTAAAGGCCTGAACCAGATC  
               
               
                 GGCGATTACAAATACTACTTCAACAGCGATGGCGTGATGCAGAAAGGCTTTGTGAGCAT 
               
               
                 CAACGATAACAAACACTATTTCGATGATAGCGGTGTGATGAAAGTGGGCTATACCGAAA  
               
               
                 TTGATGGCAAACATTTCTACTTCGCGGAAAACGGCGAAATGCAGATTGGCGTGTTCAAT 
               
               
                 ACCGAAGATGGTTTCAAATACTTCGCGCACCATAACGAAGATCTGGGTAACGAAGAAG 
               
               
                 GCGAAGAAATTAGCTATAGCGGCATCCTGAACTTCAACAACAAAATCTACTACTTTGATG 
               
               
                 ATAGCTTTACCGCGGTGGTGGGCTGGAAAGATCTGGAAGATGGCAGCAAATATTATTTC  
               
               
                 GATGAAGATACCGCGGAAGCGTATATTGGCCTGAGCCTGATTAACGATGGCCAGTACT 
               
               
                 ATTTTAACGATGATGGCATTATGCAGGTGGGTTTCGTGACCATTAATGATAAAGTGTTCT 
               
               
                 ATTTCAGCGATAGCGGCATTATTGAAAGCGGCGTGCAGAACATTGATGATAACTACTTC  
               
               
                 TACATCGATGATAACGGCATTGTGCAGATCGGCGTTTTTGATACCAGCGATGGCTACAA  
               
               
                 ATATTTCGCACCGGCCAATACCGTGAACGATAACATTTATGGCCAGGCGGTGGAATATA  
               
               
                 GCGGTCTGGTGCGTGTGGGCGAAGATGTGTATTATTTCGGCGAAACCTATACCATCGA  
               
               
                 AACCGGCTGGATTTATGATATGGAAAACGAAAGCGATAAATATTACTTTAATCCGGAAAC  
               
               
                 GAAAAAAGCGTGCAAAGGCATTAACCTGATCGATGATATCAAATACTATTTTGATGAAAA  
               
               
                 AGGCATTATGCGTACCGGTCTGATTAGCTTCGAAAACAACAACTATTACTTCAACGAAAA  
               
               
                 CGGTGAAATGCAGTTCGGCTACATCAACATCGAAGATAAAATGTTCTACTTCGGCGAAG 
               
               
                 ATGGTGTTATGCAGATTGGTGTTTTTAACACCCCGGATGGCTTCAAATACTTTGCCCATC  
               
               
                 AGAATACCCTGGATGAAAATTTCGAAGGTGAAAGCATTAACTATACCGGCTGGCTGGAT 
               
               
                 CTGGATGAAAAACGCTACTACTTCACCGATGAATACATTGCGGCGACCGGCAGCGTGA  
               
               
                 TTATTGATGGCGAAGAATACTACTTCGATCCGGATACCGCGCAGCTGGTGATTAGCGAA  
               
               
                 CATCATCATCATCACCAT 
               
               
                   
               
               
                 SEQ ID NO: 28 amino acid sequence of F5 ToxB  
               
               
                 MATGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTD  
               
               
                 ANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKY  
               
               
                 YFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQ  
               
               
                 NKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEA  
               
               
                 ATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDA  
               
               
                 NNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKK 
               
               
                 YYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQN 
               
               
                 RFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIG 
               
               
                 VFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYF 
               
               
                 MPDTAMAAAGGLFEIDGVIYFFGVDGVKAVSGLIYINDSLYYFKPPVNNLITGFVTVGDDKYY  
               
               
                 FNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFSTEDGFKYFAPANTLDENLEGEAIDFTGK 
               
               
                 LIIDENIYYFDDNYRGAVEWKELDGEMHYFSPETGKAFKGLNQIGDYKYYFNSDGVMQKGF 
               
               
                 VSINDNKHYFDDSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNE  
               
               
                 EGEEISYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFN 
               
               
                 DDGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPAN 
               
               
                 TVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINL 
               
               
                 IDDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTP 
               
               
                 DGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQ  
               
               
                 LVISEHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 29-nucleotide sequence of F52 new  
               
               
                 ATGGCAACCGGTTGGCAGACCATCGATGGCAAAAAATATTATTTTAATACCAACACCGC  
               
               
                 AATTGCAAGCACCGGCTATACCATTATCAACGGCAAACACTTTTATTTTAACACCGACG 
               
               
                 GCATTATGCAGATTGGTGTGTTTAAAGGTCCGAACGGCTTTGAATACTTTGCACCGGCA  
               
               
                 AATACCGATGCCAATAATATTGAAGGCCAGGCCATTCTGTATCAGAATGAATTTCTGAC  
               
               
                 CCTGAACGGCAAAAAATACTACTTTGGCAGCGATAGCAAAGCAGTTACCGGTTGGCGC  
               
               
                 ATCATCAACAATAAGAAATATTACTTCAACCCGAATAATGCAATTGCAGCAATTCATCTG 
               
               
                 TGCACCATTAACAACGACAAATATTATTTCAGCTATGACGGTATTCTGCAGAATGGCTAC  
               
               
                 ATTACCATCGAACGCAACAACTTTTATTTCGATGCCAACAACGAAAGCAAAATGGTGAC  
               
               
                 CGGTGTTTTCAAAGGCCCTAATGGTTTTGAGTATTTCGCTCCGGCAAACACCCATAATA  
               
               
                 ACAACATTGAAGGTCAGGCGATCGTTTATCAGAACAAATTCCTGACGCTGAATGGTAAG 
               
               
                 AAATACTATTTCGATAATGACAGCAAAGCCGTGACCGGCTGGCAGACAATTGACGGGA  
               
               
                 AGAAATATTACTTTAATCTGAATACCGCAGAAGCAGCAACCGGTTGGCAAACGATCGAC  
               
               
                 GGTAAAAAGTACTACTTCAACCTGAACACAGCCGAAGCAGCCACAGGATGGCAGACTA  
               
               
                 TTGATGGAAAAAAATACTATTTCAACACCAACACCTTTATTGCATCTACCGGTTATACCA  
               
               
                 GCATTAACGGTAAACATTTCTACTTCAACACCGATGGTATCATGCAGATCGGCGTTTTCA  
               
               
                 AAGGTCCAAATGGTTTCGAATACTTTGCCCCTGCCAATACAGATGCAAATAACATCGAG 
               
               
                 GGTCAGGCAATCCTGTACCAAAACAAATTTCTGACCCTGAATGGGAAAAAATATTACTTT 
               
               
                 GGTAGCGATTCTAAAGCCGTTACCGGTCTGCGTACCATTGATGGTAAAAAATACTACTT 
               
               
                 TAATACGAATACAGCCGTTGCGGTTACAGGCTGGCAGACCATTAACGGGAAAAAATACT 
               
               
                 ATTTTAACACAAATACCAGCATTGCCTCAACGGGTTATACCATTATTTCGGGTAAACACT 
               
               
                 TCTACTTTAATACCGATGGTATTATGCAAATCGGAGTCTTTAAAGGACCTGATGGGTTCG 
               
               
                 AATATTTTGCGCCTGCGAACACTGATGCGAACAATATCGAAGGACAGGCAATCCGCTAT 
               
               
                 CAGAATCGCTTTCTGTATCTGCACGACAACATCTATTATTTTGGCAACAATTCAAAAGCA  
               
               
                 GCCACCGGCTGGGTTACAATTGATGGCAACCGCTACTATTTCGAACCGAATACCGCAAT 
               
               
                 GGGTGCAAATGGCTACAAAACCATCGATAATAAAAATTTCTATTTTCGCAACGGTCTGC  
               
               
                 CGCAGATCGGGGTATTTAAAGGTAGCAACGGCTTCGAATACTTCGCTCCAGCGAATAC  
               
               
                 GGACGCGAACAATATTGAGGGTCAAGCGATTCGTTATCAAAACCGTTTTCTGCATCTGC  
               
               
                 TGGGCAAAATCTACTACTTTGGCAATAACAGTAAAGCAGTTACTGGATGGCAGACAATC  
               
               
                 AATGGTAAAGTGTACTATTTTATGCCGGATACCGCCATGGCAGCAGCCGGTGGTCTGTT 
               
               
                 TGAAATTGATGGCGTGATCTATTTTTTTGGTGTGGATGGTGTTAAAGCAGTGAAAGGCC  
               
               
                 TGAACCAGATCGGCGATTACAAATACTACTTCAACAGCGATGGCGTGATGCAGAAAGG 
               
               
                 CTTTGTGAGCATCAACGATAACAAACACTATTTCGATGATAGCGGTGTGATGAAAGTGG 
               
               
                 GCTATACCGAAATTGATGGCAAACATTTCTACTTCGCGGAAAACGGCGAAATGCAGATT 
               
               
                 GGCGTGTTCAATACCGAAGATGGTTTCAAATACTTCGCGCACCATAACGAAGATCTGGG 
               
               
                 TAACGAAGAAGGCGAAGAAATTAGCTATAGCGGCATCCTGAACTTCAACAACAAAATCT 
               
               
                 ACTACTTTGATGATAGCTTTACCGCGGTGGTGGGCTGGAAAGATCTGGAAGATGGCAG 
               
               
                 CAAATATTATTTCGATGAAGATACCGCGGAAGCGTATATTGGCCTGAGCCTGATTAACG 
               
               
                 ATGGCCAGTACTATTTTAACGATGATGGCATTATGCAGGTGGGTTTCGTGACCATTAAT 
               
               
                 GATAAAGTGTTCTATTTCAGCGATAGCGGCATTATTGAAAGCGGCGTGCAGAACATTGA  
               
               
                 TGATAACTACTTCTACATCGATGATAACGGCATTGTGCAGATCGGCGTTTTTGATACCA  
               
               
                 GCGATGGCTACAAATATTTCGCACCGGCCAATACCGTGAACGATAACATTTATGGCCAG 
               
               
                 GCGGTGGAATATAGCGGTCTGGTGCGTGTGGGCGAAGATGTGTATTATTTCGGCGAAA  
               
               
                 CCTATACCATCGAAACCGGCTGGATTTATGATATGGAAAACGAAAGCGATAAATATTACT 
               
               
                 TTAATCCGGAAACGAAAAAAGCGTGCAAAGGCATTAACCTGATCGATGATATCAAATAC  
               
               
                 TATTTTGATGAAAAAGGCATTATGCGTACCGGTCTGATTAGCTTCGAAAACAACAACTAT 
               
               
                 TACTTCAACGAAAACGGTGAAATGCAGTTCGGCTACATCAACATCGAAGATAAAATGTT 
               
               
                 CTACTTCGGCGAAGATGGTGTTATGCAGATTGGTGTTTTTAACACCCCGGATGGCTTCA  
               
               
                 AATACTTTGCCCATCAGAATACCCTGGATGAAAATTTCGAAGGTGAAAGCATTAACTATA  
               
               
                 CCGGCTGGCTGGATCTGGATGAAAAACGCTACTACTTCACCGATGAATACATTGCGGC  
               
               
                 GACCGGCAGCGTGATTATTGATGGCGAAGAATACTACTTCGATCCGGATACCGCGCAG 
               
               
                 CTGGTGATTAGCGAACATCATCATCATCACCAT 
               
               
                   
               
               
                 SEQ ID NO: 30-amino acid sequence of F52 New  
               
               
                 MATGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTD  
               
               
                 ANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKY  
               
               
                 YFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQ  
               
               
                 NKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTAEA  
               
               
                 ATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDA  
               
               
                 NNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKK 
               
               
                 YYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQN 
               
               
                 RFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIG 
               
               
                 VFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYF 
               
               
                 MPDTAMAAAGGLFEIDGVIYFFGVDGVKAVKGLNQIGDYKYYFNSDGVMQKGFVSINDNKH 
               
               
                 YFDDSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYS  
               
               
                 GILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQV  
               
               
                 GFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYG 
               
               
                 QAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYF 
               
               
                 DEKGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA  
               
               
                 HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVISEHHH 
               
               
                 HHH 
               
               
                   
               
               
                 SEQ ID NO: 31-amino acid sequence of Toxin A  
               
               
                 MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENKYLQLKKLNESIDVFMN 
               
               
                 KYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWIGGEVSDIALEYIKQWADI 
               
               
                 NAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEIQNPQFDNMKFYKKRMEFIYD  
               
               
                 RQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDETVLESYRTNSLRKINSNHGIDIR  
               
               
                 ANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLALKNFGGVYLDVDMLPGIHSDLFKTI 
               
               
                 SRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENFDKLDQQLKDNFKLIIESKSEKSEIFS  
               
               
                 KLENLNVSDLEIKIAFALGSVINQALISKQGSYLTNLVIEQVKNRYQFLNQHLNPAIESD  
               
               
                 NNFTDTTKIFHDSLFNSATAENSMFLTKIAPYLQVGFMPEARSTISLSGPGAYASAYYDF 
               
               
                 INLQENTIEKTLKASDLIEFKFPENNLSQLTEQEINSLWSFDQASAKYQFEKYVRDYTGG 
               
               
                 SLSEDNGVDFNKNTALDKNYLLNNKIPSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLF 
               
               
                 SKNPKNSIIIQRNMNESAKSYFLSDDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEF 
               
               
                 NTSEFARLSVDSLSNEISSFLDTIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLS  
               
               
                 IMDKITSTLPDVNKNSITIGANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYI 
               
               
                 FFDSIDNKLKAKSKNIPGLASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYY  
               
               
                 EKLEPVKNIIHNSIDDLIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSV  
               
               
                 RFINKSNGESVYVETEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNT 
               
               
                 LNAAFFIQSLIDYSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDT 
               
               
                 INVLPTITEGIPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAAT 
               
               
                 VASIVGIGAEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKT 
               
               
                 EDDKILVPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIP 
               
               
                 SLSIYSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLY  
               
               
                 PGKFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA  
               
               
                 GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDINK 
               
               
                 NKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNYLISN 
               
               
                 LSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILEFYNDST 
               
               
                 LEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKVNGLYLNES  
               
               
                 VYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTLVGKTNLGYVE  
               
               
                 FICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDISTSLDFSYEPLYG 
               
               
                 IDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKVNINLDSSSFEYKWS  
               
               
                 TEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKDIKKLSLGYIMSNFKSF 
               
               
                 NSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFYFDPIEFNLVTGWQTINGK 
               
               
                 KYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPDGFEYFAPANTQNNNIEGQA1  
               
               
                 VYQSKFLTLNGKKYYFDNNSKAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYYFNPD  
               
               
                 TAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSTSNGFEYFA  
               
               
                 PANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNSKAVTGLQTIDSKKYYFNTNTAEAATG 
               
               
                 WQTIDGKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQ 
               
               
                 IGVFKGPNGFEYFAPANTDANNIEGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNK 
               
               
                 KYYFNPNNAIAAIHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKG 
               
               
                 PNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNL 
               
               
                 NTAEAATGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFY  
               
               
                 FNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILYQNKFLTLNGKKYYFGSDSKAVT 
               
               
                 GLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIM  
               
               
                 QIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDG 
               
               
                 NRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAI 
               
               
                 RYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGV  
               
               
                 DGVKAPGIYG 
               
               
                   
               
               
                 SEQ ID NO: 32-amino acid sequence of Toxin B  
               
               
                 MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVEKYLKLKDINSLTDIYI 
               
               
                 DTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVWIGGQINDTAINYINQWKD  
               
               
                 VNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFRENLNDPRFDYNKFFRKRMEIIY  
               
               
                 DKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEIDELNTYIEESLNKITQNSGNDV  
               
               
                 RNFEEFKNGESFNLYEQELVERWNLAAASDILRISALKEIGGMYLDVDMLPGIQPDLFES  
               
               
                 IEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEHFDMLDEEVQSSFESVLASKSDKSEIF 
               
               
                 SSLGDMEASPLEVKIAFNSKGIINQGLISVKDSYCSNLIVKQIENRYKILNNSLNPAISE  
               
               
                 DNDFNTTTNTFIDSIMAEANADNGRFMMELGKYLRVGFFPDVKTTINLSGPEAYAAAYQD  
               
               
                 LLMFKEGSMNIHLIEADLRNFEISKTNISQSTEQEMASLWSFDDARAKAQFEEYKRNYFE  
               
               
                 GSLGEDDNLDFSQNIVVDKEYLLEKISSLARSSERGYIHYIVQLQGDKISYEAACNLFAK 
               
               
                 TPYDSVLFQKNIEDSEIAYYYNPGDGEIQEIDKYKIPSIISDRPKIKLTF1GHGKDEFNT 
               
               
                 DIFAGFDVDSLSTEIEAAIDLAKEDISPKSIElNLLGCNMFSYSINVEETYPGKLLLKVK 
               
               
                 DKISELMPSISQDSIIVSANQYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISF 
               
               
                 NPKENKITVKSKNLPELSTLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEER  
               
               
                 IEEAKNLTSDSINYIKDEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRF 
               
               
                 INKETGESIFVETEKTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLN 
               
               
                 AAFFIQSLIEYNSSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETID  
               
               
                 LLPTLSEGLPIIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIIT 
               
               
                 SSLGIASGFSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLD  
               
               
                 DKIMMPQDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHL 
               
               
                 SIYDVLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEG 
               
               
                 EFYWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG 
               
               
                 GTYALSLSQYNMCINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIEEN 
               
               
                 KitLNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKILMLNS  
               
               
                 NHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLISKVYMD  
               
               
                 DSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKLNSVHLDES  
               
               
                 GVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANFIISGTTSIGQ  
               
               
                 FEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGDISSTVINFSQKY  
               
               
                 LYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYINEKINVNINDLSIRY  
               
               
                 VWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNFSDKQDVPVSEIILSFT 
               
               
                 PSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSLYYFKPPVNNLITGFVTVG 
               
               
                 DDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFSTEDGFKYFAPANTLDENLEG 
               
               
                 EAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYFSPETGKAFKGLNQIGDYKYYFN 
               
               
                 SDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFA  
               
               
                 HHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIG 
               
               
                 LSLINDGQYYFNDDGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIG 
               
               
                 VFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESD  
               
               
                 KYYFNPETKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIED  
               
               
                 KMFYFGEDGVMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYI 
               
               
                 AATGSVIIDGEEYYFDPDTAQLVISE  
               
               
                   
               
               
                 SEQ ID NO: 33-amino acid sequence of CDTb″ C39 when expressed in fusion with  
               
               
                 GST.  
               
               
                 LM S DWEDEDLDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEHASGSFDKA  
               
               
                 IKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTESNTAGVSVNVGYQNGFTANVTTNYSH 
               
               
                 TTDNSTAVQDSNGESWNTGLSINKGESAYINANVRYYNTGTAPHYEVTPTTNLVLDGDTLSTIKAQENQIGNNLS  
               
               
                 PGDTYPKKGLSPLALNTMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYISQ  
               
               
                 IDSISASITLDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYFNDIPIDESCVELIFDDNT 
               
               
                 ANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNETTYNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIP 
               
               
                 MSELKPYKRYVFSGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIETTLIGSGTTYLD  
               
               
                 NLSITELNSTPEILDEPEVKIPTDQEINDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEALDYIQKYRVEAT 
               
               
                 LQYSGEKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYKKLRIYAITPDDRELLVLSVD  
               
               
                 Remarks:  
               
               
                 The protein tested in the cytotoxicity assay was obtained after cleavage of the  
               
               
                 GST by PreScission protease  
               
               
                 Following experimental results, it is demonstrated that the mature CDTb  
               
               
                 (without SP and pro-domain) starts at the Ser 212  (in red and underlined in the  
               
               
                 sequence).  
               
               
                   
               
               
                 SEQ ID NO: 34-amino acid sequence of CdtB receptor binding domain with linker in  
               
               
                 N-term of sequence, from aa 620-876 (C52)  
               
               
                 MTNFDDYNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPITSNSTIVKTKAKEE  
               
               
                 KTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIY  
               
               
                 FADLNFNPSTGNTYINGMYFAPTQTNKEALDYIQKYRVEATLQYSGEKDIGTKDKEMRNYLGDPEQPKTNYVNLR  
               
               
                 SYFTGGENIMTYKKLRIYAITPDDRELLVLSVDGGHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 35-Nucleotide sequence of C52  
               
               
                 ATGACCAATTTTGATGATTATAACAATTATCCGAGCACTTGGAGCAATGTGAATACCACCAATCAGGATGGTCTG 
               
               
                 CAGGGTAGCGCAAATAAACTGAATGGTGAAACCAAAATCAAAATTCCGATGAGCGAACTGAAACCGTATAAACGT 
               
               
                 TATGTGTTTAGCGGCTATAGCAAAGATCCGCTGACCAGCAATAGCATTATTGTGAAAATCAAAGCCAAAGAAGAA  
               
               
                 AAAACCGATTATCTGGTTCCGGAACAGGGTTATACCAAATTTAGCTATGAATTTGAAACCACCGAAAAAGATAGC  
               
               
                 AGTAATATTGAAATTACCCTGATTGGTAGCGGCACCACCTATCTGGATAATCTGAGTATTACCGAACTGAATAGC  
               
               
                 ACACCGGAAATTCTGGATGAACCCCAAGTGAAAATTCCGACCGATCAAGAAATTATGGATGCCCATAAAATCTAT 
               
               
                 TTTGCCGATCTGAACTTTAATCCGAGCACCGGCAATACCTATATTAACGGCATGTATTTTGCACCGACCCAGACC  
               
               
                 AATAAAGAAGCCCTGGATTATATTCAGAAATATCGTGTTGAAGCCACCCTGCAGTATAGCGGTTTTAAAGATATT 
               
               
                 GGCACCAAAGATAAAGAAATGCGTAATTATCTGGGCGATCCGAATCAGCCGAAAACCAATTATGTTAATCTGCGC  
               
               
                 AGCTATTTTACCGGTGGCGAAAACATTATGACCTACAAAAAACTGCGCATTTATGCCATTACACCGGATGATCGT 
               
               
                 GAACTGCTGGTTCTGAGCGTTGATGGCGCTCACCACCATCATCATCATTAA  
               
               
                   
               
               
                 SEQ ID NO: 36-amino acid sequence of CdtB receptor binding domain without linker  
               
               
                 in N-term of sequence, from aa 636-876 (C55)  
               
               
                 MNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVESGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKESY  
               
               
                 EFETTEKDSSNIEITLIGSGTTYLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIYEADLNENPSTGNTYIN 
               
               
                 GMYEAPTQTNKEALDYIQKYRVEATLQYSGEKDIGTEDKEMRNYLGDPNQPKTNYVELRSYFTGGENIMTYKKLR  
               
               
                 IYAITPDDRELLVLSVDGGHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 37-nucleotide sequence of C55  
               
               
                 ATGAATACCACCAATCAGGATGGTCTGCAGGGTAGCGCAAATAAACTGAATGGTGAAACCAAAATCAAAATTCCGATGAG 
               
               
                 CGAACTGAAACCGTATAAACGTTATGTGTTTAGCGGCTATAGCAAAGATCCGCTGACCAGCAATAGCATTATTGTGAAAA 
               
               
                 TCAAAGCCAAAGAAGAAAAAACCGATTATCTGGTTCCGGAACAGGGTTATACCAAATTTAGCTATGAATTTGAAACCACC  
               
               
                 GAAAAAGATAGCAGTAATATTGAAATTACCCTGATTGGTAGCGGCACCACCTATCTGGATAATCTGAGTATTACCGAACT 
               
               
                 GAATAGCACACCGGAAATTCTGGATGAACCGGAAGTGAAAATTCCGACCGATCAAGAAATTATGGATGCCCATAAAATCT 
               
               
                 ATTTTGCCGATCTGAACTTTAATCCGAGCACCGGCAATPCCTATATTAACGGCATGTATTTTGCACCGACCCAGACCAAT 
               
               
                 AAAGAAGCCCTGGATTATATTCAGAAATATCGTGTTGAAGCCACCCTGCAGTATAGCGGTTTTAAAGATATTGGCACCAA 
               
               
                 AGATAAAGAAATGCGTAATTATCTGGGCGATCCGAATCAGCCGAAAACCAATTATGTTAATCTGCGCAGCTATTTTACCG 
               
               
                 GTGGCGAAAACATTATGACCTACAAAAAACTGCGCATTTATGCCATTACACCGGATGATCGTGAACTGCTGGTTCTGAGC  
               
               
                 GTTGATGGCGGTCACCACCATCATCATCATTAA  
               
               
                   
               
               
                 SEQ ID NO: 38-amino acid sequence of CDTb prodomain sequence (long, aa43-211)  
               
               
                 (C58)  
               
               
                 MEIVNEDLLPNNGLMGYYFTDEHFKDLKLMAPIKDGELEFEEKKVDELLDKRKSDVKSIRWTGRIIPSKDGEYTL 
               
               
                 STDRDDVLMQVNTESTISNTLEVNMKEGKEYKVRIELQDKELGSIDNLSSPNLYWELDGMKKIIPEENLFLRDYS  
               
               
                 NIEKDDPFIPNNNEFDPELM  
               
               
                   
               
               
                 SEQ ID NO: 39-amino acid sequence of CDTb prodomain sequence (short, aa43-186)  
               
               
                 (C59)  
               
               
                 MEIVNEDILPNNGLMGYYTTDEHEKDLKLMAPIEDGNLEFEEKKVDKLLDKDKSDVESIRWTGRIIPSKDGEYTL 
               
               
                 STDRDDVLMQVNTESTISNTLEVNMKEGKEYKVRIELQDKNLGSIDNLSSPNLYWELDGMKKITPEENLF 
               
               
                   
               
               
                 SEQ ID NO: 40-amino acid sequence of Fusion CDTa N-term with linker (aa44-268) to  
               
               
                 CDTb receptor binding domain with linker in N term of sequence (aa621-876) (C60)  
               
               
                 CDTa part of the fusion is underlined.  
               
               
                 
                   MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
                 
               
               
                 
                   TRNYEYDYQIEANSREKEYHELRNAISKNKIDKPMYVYYFESPEKEAENKVIRTENQNEISL 
                 
               
               
                 
                   EKFNEFKETIQNKLEKQDGEKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTL 
                 
               
               
                   IEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDD TNFDDYNNYPSTWSNVNTTNQDGL 
               
               
                 QGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYT 
               
               
                 KESYEEETTEKDSSNTETTLIGSGTTYLDNLSITELNSTPEILDEPEVKIPTWEIMDAHKI 
               
               
                 YEADLNFNPSTGNTYINGMYFAPTQTNKEALDYIQKYRVEATLQYSGEKDIGTKDKEMRNYL 
               
               
                 GDPNQPKTNYVNLRSYFTGGENIMTYKKLRIYAITPDDRELLVLSVDGGHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 41-amino acid sequence of Fusion CDTa N-term with linker (aa44-268) to  
               
               
                 CDTb receptor binding domain without linker in N term of sequence (aa636-876) (C61)  
               
               
                 CDTa part of the fusion is underlined.  
               
               
                 
                   MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
                 
               
               
                 
                   TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAENKVIRTENQNEISL 
                 
               
               
                 
                   EKENEFKETIQNKLEKQDGEKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTL 
                 
               
               
                   IEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDD NTTNQDGLQGSANKLNGETKIKIP 
               
               
                 MSELKPYKRYVFSGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKESYFFETTEKDSSNI 
               
               
                 EITLIGSGTTYLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIYEADLNENPSTGNTYI 
               
               
                 NGMYFAPTQTNKEALDYIQKYRVEATLQYSGEKDIGTKDKEMRNYLGDPNQPKTNYVNLRSY  
               
               
                 FTGGENIMTYKKLRIYAITPDDRELLVLSVDGGHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 42-amino acid sequence of Fusion CDTa N-term without linker  
               
               
                 (aa44-260) to CDTb receptor binding domain with linker in N term of sequence  
               
               
                 (aa621-876) (C62)  
               
               
                 CDTa part of the fusion is underlined.  
               
               
                 
                   MVCNTTYKAPIERPEDFEKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
                 
               
               
                 
                   TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISL 
                 
               
               
                 
                   EKENEFKETIQNKLEKQDGEKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTL 
                 
               
               
                   IEQGYSIKIDKIVRIVIDGKHYIKAEASVVS TNEDDYNNYPSTWSNVNTTNQDGLQGSANKL 
               
               
                 NGETKIKIPMSELKPYKRYVFSGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKESYEFE  
               
               
                 TTEKESSNIEITLIGSGTTYLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIYEADLNE  
               
               
                 NPSTGNTYINGMYFAPTQTNKEALDYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPK 
               
               
                 TNYVNLRSYFIGGENINTYKKLRIYAITPDDRELLVLSVDCGHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 43-amino acid sequence of Fusion CDTa N-term without linker   
               
               
                 (aa44-260) to CDTb receptor binding domain without linker in N term of sequence   
               
               
                 (aa636-876) (C63)  
               
               
                 CDTa part of the fusion is underlined.  
               
               
                 
                   MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
                 
               
               
                 
                   TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISL 
                 
               
               
                 
                   EKFNEFKETIQNKLEKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGNLPYTNTNNVSTL 
                 
               
               
                   IEQGYSTKIDKIVRIVIDGKHYIKAEASVVS NTTNQDGLQGSANKLNGETKTKTPMSELKPY  
               
               
                 KRYVFSGYSKDFLTSNSIIVKIKAKEEKTDYLVFEQGYTKFSYEFETTEKDSSNIEITLIGS  
               
               
                 GTTYLDNLSITELNSTPEILDEPENKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAP 
               
               
                 TQTNKEALDYIQKYRVEATLQYSGEKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYPIGGENI 
               
               
                 MTYKKLRIYAITPDDRELLVLSVDGGHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 44-amino acid sequence of Fusion F2- CDTb receptor binding domain  
               
               
                 with linker in N term of sequence (aa621-876) (C64)  
               
               
                 F2 sequence is underlined.  
               
               
                 
                   MGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILYQNEF 
                 
               
               
                 
                   LTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANN 
                 
               
               
                 
                   ESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAE  
                 
               
               
                 AATGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNTNTFIASTGYTSINGKH FYFNTDGIMQIGVFKGPNGFE    
               
               
                 
                   YFAPANTDANNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFN 
                 
               
               
                 
                   TNTSIASTGYTIISGRHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFGNNS  
                 
               
               
                 
                   KAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQN 
                 
               
               
                 
                   RFLHLLGKIYYFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFD  
                 
               
               
                 
                   DSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGEKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTA  
                 
               
               
                 
                   VVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFY  
                 
               
               
                 
                   IDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFN 
                 
               
               
                 
                   PETKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPD  
                 
               
               
                   GFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVISE TNFDDYNNY  
               
               
                 PSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSNSIIVKIKAREEKTDYLVPEQG 
               
               
                 YTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPST 
               
               
                 GNTYINGMYFAPTQTNKEALDYIQKYRVEATLQYSGFKDIGTKDEEMRNYLGDPNQPYTNYVNLRSYFTGGENIM  
               
               
                 TYKKLRIYAITPDDRELLVLSVDGGHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 45-amino acid sequence of Fusion of F2 to CDTb receptor binding  
               
               
                 domain without linker inNterm of sequence (aa636-876) with 2 heterogeneous Gly  
               
               
                 residues between F2 and CTDb sequences (C65)  
               
               
                 F2 sequence is underlined.  
               
               
                 
                   MGWQTIDGKKYYFNTNTAIASTGYTIINGKHFYFNTDGTMQTGVFKGPNGFEYFAPANTDANNIEGQAILYQNEF 
                 
               
               
                 
                   LTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYDSYDGILQNGYITIERNNFYFDANN 
                 
               
               
                 
                   ESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAE  
                 
               
               
                 
                   AATGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFE  
                 
               
               
                 
                   YFAPANTDANNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFN 
                 
               
               
                 
                   TNTSIASTGYTIISGKHRYFNTDGIMQTGVFKGPDGFEYFAPANTDANNTEGQAIRYQNRFLYLHDNIYYFGNNS  
                 
               
               
                 
                   KAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQN 
                 
               
               
                 
                   RFLHLLGKIYYFGNNSKAVTGWQTINGKVYYDMPDTAMAAAGGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFD  
                 
               
               
                 
                   DSGVMHVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFTA  
                 
               
               
                 
                   VVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFDDSGIIESGVQNIDDNYFY  
                 
               
               
                 
                   IDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFN 
                 
               
               
                 
                   PETKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPD  
                 
               
               
                   GFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVISE GGNVNTTNQ  
               
               
                 DGLQGDANKLNGETKIKIPMSELKPYKRYVFSGYSEDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTE  
               
               
                 KDSSNIEITLIGSGTTYLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAP 
               
               
                 TQTNKEALDYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYKKLRIYAITP 
               
               
                 DDRELLVLSVDGGHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 46-amino acid sequence of CDTa without signal peptide, with two  
               
               
                 mutations (E428Q, E430Q, aa 44-463) (C67)  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQTRNYFYDYQIEAN 
               
               
                 SREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLEKFNEFKETIQNKLFKQDGFKDISLY  
               
               
                 EPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDV  
               
               
                 SKGDSWGKANYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKREPIPTNLT 
               
               
                 VYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIGSVNMSAFAKRKIVLRITIPKGSPGA  
               
               
                 YLSAIPGYAGgYgVLLNHGSKFKINKIDSYKDGTITKLIVDATLIP 
               
               
                   
               
               
                 SEQ ID NO: 47-nucleotide sequence of C67  
               
               
                 ATGGTTTGCAATACCACCTATAAAGCACCGATTGAACGTCCGGAAGATTTTCTGAAAGATAAAGAAAAAGCCAAA  
               
               
                 GAATGGGAACGCAAAGAAGCAGAACGTATTGAACAGAAACTGGAACGTAGCGAAAAAGAAGCACTGGAAAGCTAC  
               
               
                 AAAAAAGATAGCGTGGAAATTTCAAAATATAGCCAGACCCGCAATTATTTCTATGATTATCAGATTGAAGCCAAT 
               
               
                 AGCCGTGAAAAAGAATATAAAGAACTGCGCAATGCCATTAGCAAAAACAAAATTGATAAACCGATGTATGTGTAT 
               
               
                 TATTTCGAAAGTCCGGAAAAATTTGCCTTTAACAAAGTGATTCGCACCGAAAATCAGAATGAAATTAGCCTGGAA  
               
               
                 AAATTCAATGAATTTAAAGAAACCATTCAGAATAAACTGTTTAAACAGGATGGCTTTAAAGATATTTCACTGTAT 
               
               
                 GAACCGGGTAAAGGTGATGAAAAACCGACACCGCTGCTGATGCATCTGAAACTGCCTCGTAATACCGGTATGCTG 
               
               
                 CCGTATACCAATACCAATAATGTTAGCACCCTGATTGAACAGGGCTATAGCATCAAAATTGATAAAATTGTGCGC  
               
               
                 ATTGTGATTGATGGCAAACATTATATCAAAGCCGAAGCCAGCGTTGTTTCAAGCCTGGATTTTAAAGATGATGTG 
               
               
                 AGCAAAGGCGATAGCTGGGGTAAAGCAAACTATAATGATTGGAGCAATAAACTGACCCCGAATGAACTGGCAGAT 
               
               
                 GTGAATGATTATATGCGTGGTGGTTATACCGCCATTAACAATTATCTGATTAGCAATGGTCCGGTGAATAATCCG 
               
               
                 AATCCGGAACTGGATAGCAAAATTACCAATATTGAAAATGCCCTGAAACGCGAACCGATTCCGACCAATCTGACC  
               
               
                 GTTTATCGTCGTAGCGGTCCGCAAGAATTTGGTCTGACCCTGACCAGTCCGGAATATGACTTTAACAAACTGGAA  
               
               
                 AATATTGATGCCTTTAAAAGCAAATGGGAAGGTCAGGCACTGAGCTATCCGAACTTTATTAGCACCAGCATTGGT 
               
               
                 AGCGTTAATATGAGCGCATTTGCCAAACGTAAAATTGTGCTGCGTATTACCATTCCGAAAGGTAGTCCGGGTGCA  
               
               
                 TATCTGAGCGCAATTCCGGGTTATGCCGGT C AATAT C A G GTTCTGCTGAATCATGGCAGCAAATTCAAAATTAAC  
               
               
                 AAAATTGATAGCTATAAAGATGGCACCATTACCAAACTGATTGTTGATGCAACCCTGATTCCGTAA  
               
               
                   
               
               
                 SEQ ID NO: 48-amino acid sequence of CDTa without signal peptide, with seven  
               
               
                 mutations (R345A, Q350A, N385A, R402A, S388F, E428Q, E430Q, aa 44-463) (C69)  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
               
               
                 TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFFSPEKFAFNKVIRTENQNEISL 
               
               
                 EKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTL 
               
               
                 IEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKANYNDWSNKLTPNEL 
               
               
                 ADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKREPIPTNLTVY   A   RSGP   A   EF 
               
               
                 GLTLTSPEYDFNKLENIDAFKSKWEGQALSYP   A   FI   F   TSIGSVNMSAFAKAKIVLRITIPKGS  
               
               
                 PGAYLSAIPGYAG   Q   Y   Q   VLLNHGSKFKINKIDSYKDGTITKLIVDATLIP 
               
               
                   
               
               
                 SEQ ID NO: 49-nucleotide sequence of C69  
               
               
                 ATGGTTTGCAATACCACCTATAAAGCACCGATTGAACGTCCGGAAGATTTTCTGAAAGATAA  
               
               
                 AGAAAAAGCCAAAGAATGGGAACGCAAAGAAGCAGAACGTATTGAACAGAAACTGGAACGTA  
               
               
                 GCGAAAAAGAAGCACTGGAAAGCTACAAAAAAGATAGCGTGGAAATTTCAAAATATAGCCAG 
               
               
                 ACCCGCAATTATTTCTATGATTATCAGATTGAAGCCAATAGCCGTGAAAAAGAATATAAAGA  
               
               
                 ACTGCGCAATGCCATTAGCAAAAACAAAATTGATAAACCGATGTATGTGTATTATTTCGAAA  
               
               
                 GTCCGGAAAAATTTGCCTTTAACAAAGTGATTCGCACCGAAAATCAGAATGAAATTAGCCTG 
               
               
                 GAAAAATTCAATGAATTTAAAGAAACCATTCAGAATAAACTGTTTAAACAGGATGGCTTTAA  
               
               
                 AGATATTTCACTGTATGAACCGGGTAAAGGTGATGAAAAACCGACACCGCTGCTGATGCATC  
               
               
                 TGAAACTGGCTCGTAATACCGGTATGCTGCCGTATACCAATACCAATAATGTTAGCACCCTG 
               
               
                 ATTGAACAGGGCTATAGCATCAAAATTGATAAAATTGTGCGCATTGTGATTGATGGCAAACA  
               
               
                 TTATATCAAAGCCGAAGCCAGCGTTGTTTCAAGCCTGGATTTTAAAGATGATGTGAGCAAAG 
               
               
                 GCGATAGCTGGGGTAAAGCAAACTATAATGATTGGAGCAATAAACTGACCCCGAATGAACTG 
               
               
                 GCAGATGTGAATGATTATATGCGIGGTGGTTATACCGCCATTAACAATTATCTGATTAGCAA  
               
               
                 TGGTCGGGTGAATAATCCGAATCCGGAACTGGATAGCAAAATTACCAATATTGAAAATGCCC  
               
               
                 TGAAACGCGAACCGATTCCGACCAATCTGACCGTTTATGCACGTAGGGGTCCGGCAGAATTT 
               
               
                 GGTCTGACCCTGACCAGTCCGGAATATGACTTTAACAAACTGGAAAATATTGATGCCTTTAA  
               
               
                 AAGCAAATGGGAAGGTCAGGCACTGAGCTATCCGGCATTTATTTTCACCAGCATTGGTAGCG 
               
               
                 TTAATATGAGCGCATTTGCCAAAGCAAAAATTGTGCTGCGTATTACCATTCCGAAAGGTAGT 
               
               
                 CCGGGTGCATATCTGAGCGCAATTCCGGGTTATGCCGGTCAGTATCAGGTTCTGCTGAATCA  
               
               
                 TGGCAGCAAATTCAAAATTAACAAAATTGATAGCTATAAAGATGGCACCATTACCAAACTGA  
               
               
                 TTGTTGATGCAACCCTGATTCCG 
               
               
                   
               
               
                 SEQ ID NO: 50-amino acid sequence of CDTb without signal sequence and  
               
               
                 prodomain (mature fragment based on MS data) with Ca2+ binding motif mutation  
               
               
                 (aa212-876, mut Asp-9-11-13 Ala) (C97)  
               
               
                 3 mutated residues in this sequence. Three Asp residues were mutated into Ala.  
               
               
                 They are highlighted in bold and underlined.  
               
               
                 MSDWEDEDL   A   T   A   N   A   NIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKMVSNYLESNTAGDPYTDYEKASGSFDKAI 
               
               
                 KTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTESNTAGVSVNVGYQNGFTANVTTNYSHT 
               
               
                 TDNSTAVQDSNGESWNTGLSINKGESAYINANVRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSP 
               
               
                 GDTYPKKGLSPLALNTMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYISQI 
               
               
                 DSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYFNDIPIDESCVELIFDDNTA  
               
               
                 NKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTMFDDYNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPM  
               
               
                 SELKPYKRYVFSGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDN 
               
               
                 LSITELNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYTAPTQTNKEALDYIQKYRVEATL 
               
               
                 QYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYKKLRIYAITPDDRELLVLSVDHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 51-amino acid sequence of CDTb with prodomain removed  
               
               
                 (CDTb″, aa212-876) (C55)  
               
               
                 MSDWEDEDLDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEKASGSFDKAI 
               
               
                 KTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTESNTAGVSVNVGYQNGFTANVTTNYSHT 
               
               
                 TDNSTAVQDSNGESWNTGLSINKGESAYINANVRYYNTGTAPHYKVTPTTNLVLDGDTESTIKAQENQIGNNLSP 
               
               
                 GDTYPKKGLSPLALNTMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWEDYISQI 
               
               
                 DSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKEGLLYFNDIPIDESCVELIFDDNTA  
               
               
                 NKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDDYNNYPSTWSNVNTTNQDGLQGSANKENGETKIKIPM  
               
               
                 SELKPYKRYVFSGYSKDPLTSNSIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDN 
               
               
                 LSITELNSTPEILDEPEVKIPTDQEIMDAHKIYEADLNFNPSTGNTYINGMYFAPTQTNKEALDYTQKYRVEATE  
               
               
                 QYSGFKDIGTKDKEMPNYLGDPNQPKTNYVNLRSYFTGGENIMTYKKLRIYATTPDDRELLVLSVEHHHHHH 
               
               
                   
               
               
                 SEQ ID NO: 52-amino acid sequence of CDTa without signal peptide, with five  
               
               
                 mutations (R345A, Q350A, N385A, R402A, S388F, aa 44-463) (C107)  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKYSQ  
               
               
                 TRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTENQNEISLE  
               
               
                 KFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLPYTNTNNVSTLIE  
               
               
                 QGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKANYNDWSNKLTPNELAD  
               
               
                 VNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKREPIPTNLTVYARSGPAEFGLT 
               
               
                 LTSPEYDFNKLENIDAFKSKWEGQALSYPAFIFTSIGSVNMSAFAKAKIVLRITIPKGSPGAYL 
               
               
                 SAIPGYAGEYEVLLNHGSKFKINKIDSYKDGTITKLIVDATLIPHHHHHH**  
               
               
                   
               
               
                 SEQ ID NO: 53-Polynucleotide sequence of CDTa without signal peptide, with five  
               
               
                 mutations (R345A, Q350A, N385A, R402A, S388F, aa 44-463) (C107)  
               
               
                 ATGGTTTGCAATACCACCTATAAAGCACCGATTGAACGTCCGGAAGATTTTCTGAAAGA  
               
               
                 TAAAGAAAAAGCCAAAGAATGGGAACGCAAAGAAGCAGAACGTATTGAACAGAAACTG 
               
               
                 GAACGTAGCGAAAAAGAAGCACTGGAAAGCTACAAAAAAGATAGCGTGGAAATTTCAAA  
               
               
                 ATATAGCCAGACCCGCAATTATTTCTATGATTATCAGATTGAAGCCAATAGCCGTGAAAA  
               
               
                 AGAATATAAAGAACTGCGCAATGCCATTAGCAAAAACAAAATTGATAAACCGATGTATGT 
               
               
                 GTATTATTTCGAAAGTCCGGAAAAATTTGCCTTTAACAAAGTGATTCGCACCGAAAATCA  
               
               
                 GAATGAAATTAGCCTGGAAAAATTCAATGAATTTAAAGAAACCATTCAGAATAAACTGT 
               
               
                 TTAAACAGGATGGCTTTAAAGATATTTCACTGTATGAACCGGGTAAAGGTGATGA  
               
               
                 AAAACCGACACCGCTGCTGATGCATCTGAAACTGCCTCGTAATACCGGTATGCTG 
               
               
                 CCGTATACCAATACCAATAATGTTAGCACCCTGATTGAACAGGGCTATAGCATCA  
               
               
                 AAATTGATAAAATTGTGCGCATTGTGATTGATGGCAAACATTATATCAAAGCCGA  
               
               
                 AGCCAGCGTTGTTTCAAGCCTGGATTTTAAAGATGATGTGAGCAAAGGCGATAG 
               
               
                 CTGGGGTAAAGCAAACTATAATGATTGGAGCAATAAACTGACCCCGAATGAACT 
               
               
                 GGCAGATGTGAATGATTATATGCGTGGTGGTTATACCGCCATTAACAATTATCTG 
               
               
                 ATTAGCAATGGTCCGGTGAATAATCCGAATCCGGAACTGGATAGCAAAATTACC  
               
               
                 AATATTGAAAATGCCCTGAAACGCGAACCGATTCCGACCAATCTGACCGTTTATG 
               
               
                 CACGTAGCGGTCCGGCAGAATTTGGTCTGACCCTGACCAGTCCGGAATATGACTT 
               
               
                 TAACAAACTGGAAAATATTGATGCCTTTAAAAGCAAATGGGAAGGTCAGGCACT 
               
               
                 GAGCTATCCGGCATTTATTTTCACCAGCATTGGTAGCGTTAATATGAGCGCATTT 
               
               
                 GCCAAAGCAAAAATTGTGCTGCGTATTACCATTCCGAAAGGTAGTCCGGGTGCA  
               
               
                 TATCTGAGCGCAATTCCGGGTTATGCCGGTgAaTATgAaGTTCTGCTGAATCATGG 
               
               
                 CAGCAAATTCAAAATTAACAAAATTGATAGCTATAAAGATGGCACCATTACCAA  
               
               
                 ACTGATTGTTGATGCAACCCTGATTCCGCACCACCATCATCATCATTAATAA  
               
               
                   
               
               
                 SEQ ID NO: 54-Amino acid sequence of CDTa without signal peptide, with six  
               
               
                 mutations (R345A, Q350A, N385A, R402A, S388F, E430Q, aa 44-463) (C108)  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKFWERKEAERIEQKLERSEKEALESYKKDSVEI 
               
               
                 SKYSQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIR  
               
               
                 TENQNEISLEKFNEFKETIQNKLEKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTG 
               
               
                 MLPYTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSW  
               
               
                 GKANYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIE  
               
               
                 NALKREPIPTNLTVYARSGPAEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPAFIF 
               
               
                 TSIGSVNMSAFAKAKIVLRITIPKGSPGAYLSAIPGYAGEYQVLLNHGSKFKIN 
               
               
                 KIDSYKDGTITKLIVDATLIPHHHHHH** 
               
               
                   
               
               
                 SEQ ID NO: 55-Polynucleotide sequence of CDTa without signal peptide, with six  
               
               
                 mutations (R345A, Q350A, N385A, R402A, 5388F, E430Q, aa 44-463) (C108)  
               
               
                 ATGGTTTGCAATACCACCTATAAAGCACCGATTGAACGTCCGGAAGATTTTCTGA  
               
               
                 AAGATAAAGAAAAAGCCAAAGAATGGGAACGCAAAGAAGCAGAACGTATTGAA  
               
               
                 CAGAAACTGGAACGTAGCGAAAAAGAAGCACTGGAAAGCTACAAAAAAGATAG 
               
               
                 CGTGGAAATTTCAAAATATAGCCAGACCCGCAATTATTTCTATGATTATCAGATT 
               
               
                 GAAGCCAATAGCCGTGAAAAAGAATATAAAGAACTGCGCAATGCCATTAGCAAA  
               
               
                 AACAAAATTGATAAACCGATGTATGTGTATTATTTCGAAAGTCCGGAAAAATTTG 
               
               
                 CCTTTAACAAAGTGATTCGCACCGAAAATCAGAATGAAATTAGCCTGGAAAAAT 
               
               
                 TCAATGAATTTAAAGAAACCATTCAGAATAAACTGTTTAAACAGGATGGCTTTAA  
               
               
                 AGATATTTCACTGTATGAACCGGGTAAAGGTGATGAAAAACCGACACCGCTGCT 
               
               
                 GATGCATCTGAAACTGCCTCGTAATACCGGTATGCTGCCGTATACCAATACCAAT 
               
               
                 AATGTTAGCACCCTGATTGAACAGGGCTATAGCATCAAAATTGATAAAATTGTGC  
               
               
                 GCATTGTGATTGATGGCAAACATTATATCAAAGCCGAAGCCAGCGTTGTTTCAAG 
               
               
                 CCIGGATITTAAAGATGATGTGAGCAAAGGCGATAGCTGGGGTAAAGCAAACTA  
               
               
                 TAATGATTGGAGCAATAAACTGACCCCGAATGAACTGGCAGATGTGAATGATTA  
               
               
                 TATGCGTGGTGGTTATACCGCCATTAACAATTATCTGATTAGCAATGGTCCGGTG 
               
               
                 AATAATCCGAATCCGGAACTGGATAGCAAAATTACCAATATTGAAAATGCCCTG 
               
               
                 AAACGCGAACCGATTCCGACCAATCTGACCGTTTATGCACGTAGCGGTCCGGCA  
               
               
                 GAATTTGGTCTGACCCTGACCAGTCCGGAATATGACTTTAACAAACTGGAAAATA  
               
               
                 TTGATGCCTTTAAAAGCAAATGGGAAGGTCAGGCACTGAGCTATCCGGCATTTAT 
               
               
                 TTTCACCAGCATTGGTAGCGTTAATATGAGCGCATTTGCCAAAGCAAAAATTGTG 
               
               
                 CTGCGTATTACCATTCCGAAAGGTAGTCCGGGTGCATATCTGAGCGCAATTCCGG 
               
               
                 GTTATGCCGGTgAaTATcAaGTTCTGCTGAATCATGGCAGCAAATTCAAAATTAAC  
               
               
                 AAAATTGATAGCTATAAAGATGGCACCATTACCAAACTGATTGTTGATGCAACCC  
               
               
                 TGATTCCGCACCACCATCATCATCATTAATAA  
               
               
                   
               
               
                 SEQ ID NO: 56-Amino acid sequence of CDTa without signal peptide, with six  
               
               
                 mutations (R345A, Q350A, N385A, R402A, S388F, E428Q, aa 44-463) (C110  
               
               
                 MVCNTTYKAPIERPEDFLKDKEKAKEWERKEAEKIEQKLERSEKEALESYKKDSVEI 
               
               
                 SKYSQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIR  
               
               
                 TENQNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTG 
               
               
                 MLPYTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSW  
               
               
                 GKANYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIE  
               
               
                 NALKREPIPTNLTVYARSGPAEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPAFIF 
               
               
                 TSIGSVNMSAFAKAKIVLRITIPKGSPGAYLSAIPGYAGQYEVLLNHGSKFKIN 
               
               
                 KIDSYKDGTITKLIVDATLIPHHHHHH**