Patent Publication Number: US-11649463-B2

Title: Trichome specific promoters for the manipulation of cannabinoids and other compounds in glandular trichomes

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     The present application is a Divisional of U.S. patent application Ser. No. 16/334,284, filed Mar. 18, 2019, which is the U.S. National Stage of International Patent Application No. PCT/US2017/051493, filed Sep. 14, 2017, which claims priority from U.S. Provisional Patent Application No. 62/397,212, filed Sep. 20, 2016, the contents of these applications are hereby incorporated by reference in their entirety. 
    
    
     TECHNICAL FIELD 
     The present technology relates generally to trichome specific promoters of cannabinoid biosynthesis enzyme genes from  Cannabis , nucleotide sequences of the trichome specific promoters, and uses of the promoters for modulating cannabinoid production or for modulating other trichome specific production of biochemicals in organisms. The present technology also relates to transgenic cells and organisms, including plant cells and plants, comprising the trichome specific promoters. 
     BACKGROUND 
     The following description is provided to assist the understanding of the reader. None of the information provided or references cited is admitted to be prior art. 
     Plant trichomes are epidermal protuberances, including branched and unbranched hairs, vesicles, hooks, spines, and stinging hairs covering the leaves, bracts, and stems. There are two major classes of trichomes, which may be distinguished on the basis of their capacity to produce and secrete or store secondary metabolites, namely glandular trichomes and non-glandular trichomes. Non-glandular trichomes exhibit low metabolic activity and provide protection to the plant mainly through physical means. By contrast, glandular trichomes, which are present on the foliage of many plant species including some solanaceous species (e.g., tobacco, tomato) and also  cannabis , are highly metabolically active and accumulate metabolites, which can represent up to 10-15% of the leaf dry weight (Wagner et al.,  Ann. Bot.  93:3-11 (2004)). Glandular trichomes are capable of secreting (or storing) secondary metabolites as a defense mechanism. 
       Cannabis sativa  L. ( cannabis , hemp, marijuana), an annual herb that has been cultivated for thousands of years, contains a unique set of secondary metabolites called cannabinoids, which constitute a group of terpenophenolics. Cannabinoids are primarily synthesized and accumulate in glandular trichomes that are present at high densities on female flowers and at lower densities on male flowers of  C. sativa  plants. The accumulation of cannabinoids in the storage cavity of trichomes is beneficial to the plant as cannabinoids are known to be cytotoxic to other plant cells and have been shown to induce apoptosis in both hemp and tobacco cell suspension cultures (Sirikantaramas et al.,  Plant Cell Physiol.  46:1578-1582 (2005)). Cannabinoids are formed by a three-step biosynthetic process: polyketide formation, aromatic prenylation, and cyclization ( FIG.  1   ). The cannabinoid pathway is supplied by hexanoyl-CoA, the formation of which is catalyzed by hexanoyl-CoA synthetase. The first enzymatic step in cannabinoid biosynthesis is the formation of 3,5,7-trioxododecanoyl-CoA by a tetraketide synthase enzyme (TKS), termed olivetolic acid synthase (OLS1). The second enzymatic step in cannabinoid biosynthesis is the formation of olivetolic acid by olivetolic acid cyclase (OAC). The next step is the prenylation of olivetolic acid to form cannabigerolic acid (CBGA) by the aromatic prenyltransferase. CBGA is a central branch-point intermediate for the biosynthesis of the different major classes of cannabinoids. Alternative cyclization of the prenyl side-chain of CBGA yields Δ 9 tetrahydrocannabinolic acid (THCA) or its isomers cannabidiolic acid (CBDA) or cannabichromenic acid (CBCA). THCA and CBDA are later decarboxylated by a non-enzymatic reaction during storage or smoking to yield Δ 9 -tetrahydrocannabinol (THC) or cannabidiol (CBD), respectively ( FIG.  1   ). 
     Cannabinoids are valuable plant-derived natural products.  Cannabis  preparations, such as marijuana and hashish, have been used for centuries for their well-known psychoactive effects. Cannabinoids have attracted a renewed interest for medical applications due to their ability to act through mammalian cannabinoid receptors. Major cannabinoids include Δ 9 -tetrahydrocannabinol (THC), the compound responsible for the psychoactive and therapeutic effects of marijuana consumption, and cannabidiol (CBD), which has neuroprotective properties. (Gaoni &amp; Mechoulam,  J. Am. Chem. Sc.  86:1646-1647 (1964): Mechoulam et al.,  J. Clin. Pharmacol.  42:11S-19S (2002)). THCA is the major cannabinoid in drug strains of  cannabis  while CBDA is the predominant cannabinoid in hemp forms grown for fiber or seed. Cannabinoids are currently being explored for therapeutic purposes, including the treatment of chronic pain, nausea, the control of spasticity and tremor in patients suffering from multiple sclerosis or epilepsy, as well as a therapy for arthritis. The possibility to direct cannabinoid production in trichomes avoids interference in the plants&#39; metabolic pathways and performance. Accordingly, there is a need to identify trichome specific promoters to modulate the synthesis of cannabinoids in organisms including transgenic plants, transgenic cells, and derivatives thereof, which allow for targeting of gene expression specifically in trichomes. 
     SUMMARY 
     Disclosed herein are trichome specific promoters and uses of these promoters for directing the expression of coding nucleic acid sequences in plant trichomes. 
     In one aspect, the present disclosure provides a synthetic DNA molecule comprising a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence set forth in any one of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33; and (b) a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of any one of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33, and which encodes a promoter having plant trichome gland specific transcriptional activity, wherein the nucleotide sequence is operably linked to a heterologous nucleic acid. 
     In some embodiments, the present disclosure provides an expression vector comprising the synthetic DNA molecule operably linked to one or more nucleic acid sequences encoding a polypeptide. 
     In some embodiments, the present disclosure provides a genetically engineered host cell comprising the expression vector. In some embodiments, the genetically engineered host cell is a  Cannabis sativa  cell. In some embodiments, the genetically engineered host cell is a  Nicotiana tabacum  cell. 
     In some embodiments, the present disclosure provides a genetically engineered plant comprising a cell comprising a chimeric nucleic acid construct comprising the synthetic DNA molecule. In some embodiments, the genetically engineered plant belongs to the family Solanacea. In some embodiments, the engineered Solanacea plant is an  N. tabacum  plant. In some embodiments, the genetically engineered plant belongs to the family Cannabaceae. In some embodiments, the engineered Cannabaceae plant is a  C. sativa  plant. In some embodiments, the present disclosure provides seeds from the genetically engineered plant, wherein the seeds comprise the chimeric nucleic acid construct. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 1. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 1, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 2. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 2, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 3. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 3, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 5. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 5, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 6. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 6, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 8. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 8, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 9. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 9, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 11. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 11, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 12. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 12, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 13. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 13, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 14. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 14, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 16. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 16, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 17. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 17, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 18. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 18, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 19. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 19, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 21. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 21, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 22. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 22, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 23. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 23, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 24. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 24, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 25. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 25, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 26. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 26, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 28. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 28, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 29. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 29, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 31. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 31, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 32. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 32, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the present disclosure provides a synthetic DNA molecule with the nucleotide sequence as set forth in SEQ ID NO: 33. In some embodiments, the present disclosure provides a synthetic DNA molecule having a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of SEQ ID NO: 33, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In one aspect, the present disclosure provides a genetically engineered plant or plant cell comprising a chimeric gene integrated into its genome, the chimeric gene comprising a trichome specific promoter operably linked to a homologous or heterologous nucleic acid sequence, wherein the promoter is selected from the group consisting of: (a) a nucleotide sequence of any one of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33; and (b) a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of any one of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33, and which encodes a promoter that has plant trichome gland specific transcriptional activity. 
     In some embodiments, the genetically engineered plant or plant cell belongs to the family Solanacea. In some embodiments, the Solanacea plant is  N. tabacum . In some embodiments, the genetically engineered plant belongs to the family Cannabaceae. In some embodiments, the engineered Cannabaceae plant is  C. sativa.    
     In one aspect, the present disclosure provides a method for expressing a polypeptide in plant trichomes, comprising: (a) introducing into a host cell an expression vector comprising a nucleotide sequence selected from the group consisting of: (i) a nucleotide sequence set forth in any one of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33; and (ii) a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of any one of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33, and which encodes a promoter that has plant trichome gland specific transcriptional activity; wherein the nucleic acid sequence of (i) or (ii) is operably linked to one or more nucleic acid sequences encoding a polypeptide; and (b) growing the plant under conditions which allow for the expression of the polypeptide. 
     In another aspect, the present disclosure provides a method for increasing a cannabinoid in a host plant trichome, comprising: (a) introducing into a host cell an expression vector comprising a nucleotide sequence selected from the group consisting of: (i) a nucleotide sequence set forth in any one of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33: and (ii) a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of any one of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33, and which encodes a promoter that has plant trichome gland specific transcriptional activity; wherein the nucleic acid sequence of (i) or (ii) is operably linked to one or more nucleic acid sequences encoding an enzyme of the cannabinoid biosynthetic pathway: and (b) growing the plant under conditions which allow for the expression of the cannabinoid biosynthetic pathway enzyme; wherein expression of the cannabinoid biosynthetic pathway enzyme results in the plant having an increased cannabinoid content relative to a control plant grown under similar conditions. 
     In some embodiments of the method, the cannabinoid biosynthetic pathway enzyme is cannabidiolic acid (CBDA) synthase, cannabichromenic acid (CBCA) synthase or Δ 9 tetrahydrocannabinolic acid (THCA) synthase. 
     In some embodiments, the method further comprises providing the plant with cannabigerolic acid (CBGA). 
     In some embodiments, the present disclosure provides a method for producing a genetically-engineered plant having increased Δ 9 tetrahydrocannabinol (THC), cannabichromene (CBC), and/or cannabidiol (CBD) content relative to a control plant. 
     The technologies described and claimed herein have many attributes and embodiments including, but not limited to, those set forth or described or referenced in this brief summary. It is not intended to be all-inclusive and the inventions described and claimed herein are not limited to or by the features or embodiments identified in this brief summary, which is included for purposes of illustration only and not restriction. Additional embodiments may be disclosed in the detailed description below. 
     In one aspect, the present disclosure provides a synthetic DNA molecule comprising a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence set forth in any one of SEQ ID NOs: 31, 32, or 33; and (b) a nucleotide sequence that is at least about 80% identical to the nucleotide sequence of any one of SEQ ID NOs: 31, 32, or 33, and which encodes a promoter having plant trichome gland specific transcriptional activity, wherein the nucleotide sequence is operably linked to a heterologous nucleic acid. 
     In some embodiments, the present disclosure provides an expression vector comprising the synthetic DNA molecule operably linked to one or more nucleic acid sequences encoding a polypeptide. 
     In some embodiments, the present disclosure provides a genetically engineered host cell comprising the expression vector. In some embodiments, the genetically engineered host cell is a  Cannabis sativa  cell. In some embodiments, the genetically engineered host cell is a  Nicotiana tabacum  cell. 
     In some embodiments, the present disclosure provides a genetically engineered plant comprising a cell comprising a chimeric nucleic acid construct comprising the synthetic DNA molecule. In some embodiments, the engineered plant is an  N. tabacum  plant. In some embodiments, the engineered plant is a  C. sativa  plant. 
     In some embodiments, the present disclosure provides seeds from the engineered plant of any one of 5, wherein the seeds comprise the chimeric nucleic acid construct. 
     In one aspect, the present disclosure provides a synthetic DNA molecule comprising a nucleotide sequence set forth in SEQ ID NO: 33. 
     In some embodiments, the present disclosure provides an expression vector comprising the synthetic DNA molecule operably linked to one or more nucleic acid sequences encoding a polypeptide. 
     In some embodiments, the present disclosure provides a genetically engineered host cell comprising the expression vector. In some embodiments, the genetically engineered host cell is a  Cannabis sativa  cell. In some embodiments, the genetically engineered host cell is a  Nicotiana tabacum  cell. 
     In some embodiments, the present disclosure provides a genetically engineered plant comprising a cell comprising a chimeric nucleic acid construct comprising the synthetic DNA molecule. In some embodiments, the engineered plant is an  N. tabacum  plant. In some embodiments, the engineered plant is a  C. sativa  plant. 
     In some embodiments, the present disclosure provides seeds from the engineered plant, wherein the seeds comprise the chimeric nucleic acid construct. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG.  1    depicts the cannabinoid biosynthetic pathway that leads to the formation of the major cannabinoids in  Cannabis sativa , Δ 9 -tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA). 
         FIGS.  2 A- 2 B  show the promoter:reporter gene design utilized in the experiments described herein. Each promoter is fused to the GUS reporter gene and promoter activity is indicated by a blue color upon staining ( FIG.  2 A ).  FIG.  2 B  is a whole leaf view of trichome specific expression of cannabinoid biosynthetic enzyme gene promoters in tobacco trichomes. Results from a representative promoter (CBDA synthase 20800 gene promoter) are shown. Results from the other promoters in the cannabinoid biosynthetic pathway are qualitatively identical (not shown).  FIG.  2 B  shows that expression of the reporter gene is restricted to the trichomes. 
         FIG.  3    shows trichome specific expression of promoters from the complete cannabinoid biosynthetic pathway in tobacco trichomes. Promoter activity is indicated by a blue color caused by activity of the GUS reporter gene. A representative promoter from every gene in the pathway shows trichome specific expression and therefore all promoters from genes in the pathway will direct expression in the trichomes. 
         FIG.  4 A  shows the design of the 4×Cannabinoid On (CANON) fragment synthetic promoter of the present technology.  FIG.  4 B  shows trichome specific expression of the 4×CANON fragment synthetic promoter of the present technology. The activity of the synthetic promoter is indicated by the blue color caused by activity of the GUS reporter gene. 
     
    
    
     DETAILED DESCRIPTION 
     I. Introduction 
     The present technology relates to the discovery of nucleic acid sequences for twenty-three trichome specific promoters of enzymes involved in the cannabinoid biosynthetic pathway: (1) olivetol synthase (OLS; also referred to as tetraketide synthase) promoter; (2) OLS1 promoter; (3) OLS2 promoter; (4) olivetolic acid cyclase (OAC) promoter; (5) OAC1 promoter; (6) aromatic prenyltransferase (PT) promoter; (7) PT1 promoter; (8) hexanoyl-CoA synthetase (AAE1-1) promoter; (9) hexanoyl-CoA synthetase (AAE1-1′) promoter; (10) hexanoyl-CoA synthetase (AAE3) promoter; (11) hexanoyl-CoA synthetase (AAE12) promoter; (12) CBDA synthase (CBDAS) promoter; (13) CBDA synthase 1 (CBDAS1) promoter; (14) CBDA synthase (CBDAS) 20800 promoter; (15) CBDA synthase (CBDAS) 20800′ promoter; (16) THCA synthase (THCAS) 19603 promoter; (17) THCA synthase (THCAS) 19603′ promoter; (18) THCA synthase (THCAS) 50320 promoter: (19) THCA synthase (THCAS) 50320′ promoter: (20) THCA synthase (THCAS) 1330 promoter; (21) THCA synthase (THCAS) 1330′ promoter; (22) CBDA synthase (CBDAS) 3498 promoter: and (23) CBDA synthase (CBDAS) 3498′ promoter. 
     The nucleic acid sequences for each promoter have been determined. The nucleic acid sequences of the (i) olivetol synthase (OLS) promoter, (ii) OLS1 promoter, and (iii) OLS2 promoter are set forth in SEQ ID NOs: 1, 2, and 3, respectively, and the open reading frame (ORF) of OLS is set forth in SEQ ID NO: 4. The nucleic acid sequence of the olivetolic acid cyclase (OAC) promoter is set forth in SEQ ID NO: 5, the nucleic acid sequence of the OAC1 promoter is set forth in SEQ ID NO: 6, and the ORF of OAC is set forth in SEQ ID NO: 7. The nucleic acid sequence of aromatic prenyltransferase (PT) promoter is set forth in SEQ ID NO: 8, the nucleic acid sequence of the PT1 promoter is set forth in SEQ ID NO: 9, and the ORF of PT is set forth in SEQ ID NO: 10. The nucleic acid sequences of the (i) hexanoyl-CoA synthetase (AAE1-1) promoter, (ii) hexanoyl-CoA synthetase (AAE1-1′) promoter: (iii) hexanoyl-CoA synthetase (AAE3) promoter, and (iv) hexanoyl-CoA synthetase (AAE12) promoter are set forth in SEQ ID NOs: 11, 12, 13, and 14, respectively, and the ORF of hexanoyl-CoA (AAE-1) is set forth in SEQ ID NO: 15. The nucleic acid sequences of the (i) CBDA synthase (CBDAS) promoter, (ii) CBDAS synthase 1 (CBDAS1) promoter, (iii) CBDA synthase (CBDAS) 20800 promoter, and (iv) CBDA synthase (CBDAS) 20800′ promoter are set forth in SEQ ID NOs: 16, 17, 18, and 19, respectively, and the nucleic acid sequence of the ORF of CBDA synthase (CBDAS) is set forth in SEQ ID NO: 20. The nucleic acid sequences of (i) THCA synthase (THCAS) 19603 promoter, (ii) THCA synthase (THCAS) 19603′ promoter, (iii) THCA synthase (THCAS) 50320 promoter, (iv) THCA synthase (THCAS) 50320′ promoter, (v) THCA synthase (THCAS) 1330 promoter, and (vi) THCA synthase (THCAS) 1330′ promoter are set forth in SEQ ID NOs: 21, 22, 23, 24, 25, and 26, respectively, and the ORF of THCAS is set forth in SEQ ID NO: 27. The nucleic acid sequence of the CBCA synthase (CBCAS) 3498 promoter is set forth in SEQ ID NO: 28, the nucleic acid sequence of the CBCA synthase (CBCAS) 3498′ promoter is set forth in SEQ ID NO: 29, and the ORF of CBCA synthase (CBCAS) is set forth in SEQ ID NO: 30. 
     The present technology also relates to the discovery of nucleic acid sequences for a “cannabinoid on” or “CANON” promoter fragment that is sufficient to direct trichome specific expression. The nucleic acid sequence for the CANON fragment that is sufficient to direct trichome specific expression in glandular trichomes is set forth in SEQ ID NO: 31. The nucleic acid sequence for the 4×CANON fragment synthetic promoter that comprises four copies of the consensus CANON fragment is set forth in SEQ ID NO: 33. 
     Given the known cytotoxic effects of cannabinoids such as THC on plant cells, the expression of genes driving their production under a strong ubiquitous promoter, like the Cauliflower Mosaic Virus (CaMV) 35S, may lead to the perturbation of metabolic pathways in the whole plant and may have deleterious consequences on plant development and physiology. Thus, trichomes, as distinct entities with restricted communication to the rest of plant, represent a potential target for metabolic engineering. 
     Accordingly, in some embodiments, the present technology provides previously undiscovered trichome specific promoters from cannabinoid biosynthesis genes or biologically active fragments thereof that may be used to genetically manipulate the synthesis of cannabinoids (e.g., THC, CBD, CBC. CBG) in host plants, such as  C. sativa , plants of the family Solanaceae, and other plant families and species that do not naturally produce cannabinoids. 
     II. Definitions 
     All technical terms employed in this specification are commonly used in biochemistry, molecular biology and agriculture; hence, they are understood by those skilled in the field to which the present technology belongs. Those technical terms can be found, for example in:  Molecular Cloning: A Laboratory Manual  3rd ed., vol. 1-3, ed. Sambrook and Russel (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 2001);  Current Protocols In Molecular Biology , ed. Ausubel et al. (Greene Publishing Associates and Wiley-Interscience, New York, 1988) (including periodic updates):  Short Protocols In Molecular Biology: A Compendium Of Methods From Current Protocols In Molecular Biology  5th ed., vol. 1-2, ed. Ausubel et al. (John Wiley &amp; Sons, Inc., 2002); Genome Analysis: A Laboratory Manual, vol. 1-2, ed. Green et al. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1997). Methodology involving plant biology techniques are described here and also are described in detail in treatises such as  Methods In Plant Molecular Biology: A Laboratory Course Manual , ed. Maliga et al. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1995). 
     A “chimeric nucleic acid” comprises a coding sequence or fragment thereof linked to a nucleotide sequence that is different from the nucleotide sequence with which it is associated in cells in which the coding sequence occurs naturally. 
     The terms “encoding” and “coding” refer to the process by which a gene, through the mechanisms of transcription and translation, provides information to a cell from which a series of amino acids can be assembled into a specific amino acid sequence to produce an active enzyme. Because of the degeneracy of the genetic code, certain base changes in DNA sequence do not change the amino acid sequence of a protein. 
     “Endogenous nucleic acid” or “endogenous sequence” is “native” to, i.e., indigenous to, the plant or organism that is to be genetically engineered. It refers to a nucleic acid, gene, polynucleotide, DNA, RNA, mRNA, or cDNA molecule that is present in the genome of a plant or organism that is to be genetically engineered. 
     “Exogenous nucleic acid” refers to a nucleic acid, DNA or RNA, which has been introduced into a cell (or the cell&#39;s ancestor) through the efforts of humans. Such exogenous nucleic acid may be a copy of a sequence which is naturally found in the cell into which it was introduced, or fragments thereof. 
     As used herein, “expression” denotes the production of an RNA product through transcription of a gene or the production of the polypeptide product encoded by a nucleotide sequence. “Overexpression” or “up-regulation” is used to indicate that expression of a particular gene sequence or variant thereof, in a cell or plant, including all progeny plants derived thereof, has been increased by genetic engineering, relative to a control cell or plant. 
     “Genetic engineering” encompasses any methodology for introducing a nucleic acid or specific mutation into a host organism. For example, a plant is genetically engineered when it is transformed with a polynucleotide sequence that suppresses expression of a gene, such that expression of a target gene is reduced compared to a control plant. In the present context, “genetically engineered” includes transgenic plants and plant cells. A genetically engineered plant or plant cell may be the product of any native approach (i.e., involving no foreign nucleotide sequences), implemented by introducing only nucleic acid sequences derived from the host plant species or from a sexually compatible plant species. See. e.g., U.S. Patent Application No. 2004/0107455. 
     “Heterologous nucleic acid” or “homologous nucleic acid” refer to the relationship between a nucleic acid or amino acid sequence and its host cell or organism, especially in the context of transgenic organisms. A homologous sequence is naturally found in the host species (e.g., a  cannabis  plant transformed with a  cannabis  gene), while a heterologous sequence is not naturally found in the host cell (e.g., a tobacco plant transformed with a sequence from  cannabis  plants). Such heterologous nucleic acids may comprise segments that are a copy of a sequence that is naturally found in the cell into which it has been introduced, or fragments thereof. Depending on the context, the term “homolog” or “homologous” may alternatively refer to sequences which are descendent from a common ancestral sequence (e.g., they may be orthologs). 
     “Increasing,” “decreasing,” “modulating,” “altering,” or the like refer to comparison to a similar variety, strain, or cell grown under similar conditions but without the modification resulting in the increase, decrease, modulation, or alteration. In some cases, this may be a non-transformed control, a mock transformed control, or a vector-transformed control. 
     By “isolated nucleic acid molecule” is intended a nucleic acid molecule, DNA, or RNA, which has been removed from its native environment. For example, recombinant DNA molecules contained in a DNA construct are considered isolated for the purposes of the present technology. Further examples of isolated DNA molecules include recombinant DNA molecules maintained in heterologous host cells or DNA molecules that are purified, partially or substantially, in solution. Isolated RNA molecules include in vitro RNA transcripts of the DNA molecules of the present technology. Isolated nucleic acid molecules, according to the present technology, further include such molecules produced synthetically. 
     “Plant” is a term that encompasses whole plants, plant organs (e.g., leaves, stems, roots, etc.), seeds, differentiated or undifferentiated plant cells, and progeny of the same. Plant material includes without limitation seeds, suspension cultures, embryos, meristematic regions, callus tissues, leaves, roots, shoots, stems, fruit, gametophytes, sporophytes, pollen, and microspores. 
     “Plant cell culture” means cultures of plant units such as, for example, protoplasts, cell culture cells, cells in plant tissues, pollen, pollen tubes, ovules, embryo sacs, zygotes, and embryos at various stages of development. In some embodiments of the present technology, a transgenic tissue culture or transgenic plant cell culture is provided, wherein the transgenic tissue or cell culture comprises a nucleic acid molecule of the present technology. 
     “Promoter” connotes a region of DNA upstream from the start of transcription that is involved in recognition and binding of RNA polymerase and other proteins to initiate transcription. A “constitutive promoter” is one that is active throughout the life of the plant and under most environmental conditions. Tissue-specific, tissue-preferred, cell type-specific, and inducible promoters constitute the class of “non-constitutive promoters.” A “trichome specific promoter” is a promoter that preferentially directs expression of an operably linked gene in trichome tissue, as compared to expression in the root, leaf, stem, or other tissues of the plant. “Operably linked” refers to a functional linkage between a promoter and a second sequence, where the promoter sequence initiates and mediates transcription of the DNA sequence corresponding to the second sequence. In general, “operably linked” means that the nucleic acid sequences being linked are contiguous. 
     “Sequence identity” or “identity” in the context of two polynucleotide (nucleic acid) or polypeptide sequences includes reference to the residues in the two sequences that are the same when aligned for maximum correspondence over a specified region. When percentage of sequence identity is used in reference to proteins it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties, such as charge and hydrophobicity, and therefore do not change the functional properties of the molecule. Where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences which differ by such conservative substitutions are said to have “sequence similarity” or “similarity.” Means for making this adjustment are well-known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, for example, according to the algorithm of Meyers &amp; Miller,  Computer Applic. Biol. Sci.  4: 11-17 (1988), as implemented in the program PC/GENE (Intelligenetics, Mountain View, Calif. USA). 
     Use in this description of a percentage of sequence identity denotes a value determined by comparing two optimally aligned sequences over a comparison window, wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison, and multiplying the result by 100 to yield the percentage of sequence identity. 
     The terms “suppression” or “down-regulation” are used synonymously to indicate that expression of a particular gene sequence variant thereof, in a cell or plant, including all progeny plants derived thereof, has been reduced by genetic engineering, relative to a control cell or plant. 
     “Trichome” encompasses herein different types of trichomes, both glandular trichomes and/or non-glandular trichomes. 
     “Trichome cells” refers to the cells making up the trichome structure, such as the gland, or secretory cells, base cells and stalk, or stripe cells, extra-cellular cavity and cuticle cells. Trichomes can also consist of one single cell. 
     “ Cannabis ” or “ cannabis  plant” refers to any species in the  Cannabis  genus that produces cannabinoids, such as  Cannabis sativa  and interspecific hybrids thereof. 
     A “variant” is a nucleotide or amino acid sequence that deviates from the standard, or given, nucleotide or amino acid sequence of a particular gene or polypeptide. The terms “isoform,” “isotype,” and “analog” also refer to “variant” forms of a nucleotide or an amino acid sequence. An amino acid sequence that is altered by the addition, removal, or substitution of one or more amino acids, or a change in nucleotide sequence, may be considered a variant sequence. A polypeptide variant may have “conservative” changes, wherein a substituted amino acid has similar structural or chemical properties, e.g., replacement of leucine with isoleucine. A polypeptide variant may have “nonconservative” changes, e.g., replacement of a glycine with a tryptophan. Analogous minor variations may also include amino acid deletions or insertions, or both. Guidance in determining which amino acid residues may be substituted, inserted, or deleted may be found using computer programs well known in the art such as Vector NTI Suite (InforMax, MD) software. Variant may also refer to a “shuffled gene” such as those described in Maxygen-assigned patents (see, e.g., U.S. Pat. No. 6,602,986). 
     As used herein, the term “about” will be understood by persons of ordinary skill in the art and will vary to some extent depending upon the context in which it is used. If there are uses of the term which are not clear to persons of ordinary skill in the art given the context in which it is used, “about” will mean up to plus or minus 10% of the particular term. 
     The term “biologically active fragments” or “functional fragments” or “fragments having promoter activity” refer to nucleic acid fragments which are capable of conferring transcription in one or more trichome types and/or one or more trichome cells found on one or more different types of plant tissues and organs. Biologically active fragments confer trichome specific and/or at least trichome preferred expression, and they preferably have at least a similar strength (or higher strength) as the promoter of SEQ ID NOs: 1, 3, 5, 7, 9, or 11-15. This can be tested by transforming a plant with such a fragment, preferably operably linked to a reporter gene, and assaying the promoter activity qualitatively (spatio-temporal transcription) and/or quantitatively in trichomes. In some embodiments, the strength of the promoter and/or promoter fragments of the present technology is quantitatively identical to, or higher than, that of the CaMV 35S promoter when measured in the glandular trichome. In some embodiments, a biologically active fragment of a trichome promoter described herein can be about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or about 99% of the full length sequence nucleic acid sequence for the promoter. In other embodiments, a biologically active nucleic acid fragment of a trichome promoter described herein can be, for example, at least about 10 contiguous nucleic acids. In yet other embodiments, the biologically active nucleic acid fragment of a trichome promoter described herein can be (1) about 10 contiguous nucleic acids up to about 554 contiguous nucleic acids for the OLS promoter (e.g., SEQ ID NO: 1); (2) about 10 contiguous nucleic acids up to about 550 contiguous nucleic acids for the OLS1 promoter (SEQ ID NO: 2); (3) about 10 contiguous nucleic acids up to about 558 contiguous nucleic acids for the OLS2 promoter (SEQ ID NO: 3); (4) about 10 contiguous nucleic acids up to about 996 contiguous nucleic acids for the OAC promoter (e.g., SEQ ID NO: 5); (5) about 10 contiguous nucleic acids up to about 992 contiguous nucleic acids for the OAC1 promoter (e.g., SEQ ID NO: 6); (6) about 10 contiguous nucleic acids up to about 1361 contiguous nucleic acids for the PT promoter (e.g., SEQ ID NO: 8): (7) about 10 contiguous nucleic acids up to about 1357 contiguous nucleic acids for the PT1 promoter (e.g., SEQ ID NO: 9): (8) about 10 contiguous nucleic acids up to about 805 contiguous nucleic acids for the AAE1-1 promoter (e.g., SEQ ID NO: 11); (9) about 10 contiguous nucleic acids up to about 800 contiguous nucleic acids for the AAE1-1′ promoter (e.g., SEQ ID NO: 12); (10) about 10 contiguous nucleic acids up to about 1000 contiguous nucleic acids for the AAE3 promoter (e.g., SEQ ID NO: 13); (11) about 10 contiguous nucleic acids up to about 869 contiguous nucleic acids for the AAE12 promoter (e.g., SEQ ID NO: 14): (12) about 10 contiguous nucleic acids up to about 420 contiguous nucleic acids for the CBDA synthase promoter (e.g., SEQ ID NO: 16): (13) about 10 contiguous nucleic acids up to about 416 contiguous nucleic acids for the CBDAS1 promoter (e.g., SEQ ID NO: 17); (14) about 10 contiguous nucleic acids up to about 535 contiguous nucleic acids for the CBDAS 20800 promoter (e.g., SEQ ID NO: 18): (15) about 10 contiguous nucleic acids up to about 531 contiguous nucleic acids for the CBDAS 20800′-promoter (e.g., SEQ ID NO: 19); (16) about 10 contiguous nucleic acids up to about 800 contiguous nucleic acids for the THCAS 19603 promoter (e.g., SEQ ID NO: 21); (17) about 10 contiguous nucleic acids up to about 796 contiguous nucleic acids for the THCAS 19603′ promoter (e.g., SEQ ID NO: 22); (18) about 10 contiguous nucleic acids up to about 796 contiguous nucleic acids for the THCAS 50320 promoter (e.g., SEQ ID NO: 23): (19) about 10 contiguous nucleic acids up to about 792 contiguous nucleic acids for the THCAS 50320′ promoter (e.g., SEQ ID NO: 24); (20) about 10 contiguous nucleic acids up to about 720 contiguous nucleic acids for the THCAS 1330 promoter (e.g., SEQ ID NO: 25); (21) about 10 contiguous nucleic acids up to about 716 contiguous nucleic acids for the THCAS 1330′ promoter (e.g., SEQ ID NO: 26): (22) about 10 contiguous nucleic acids up to about 804 contiguous nucleic acids for the CBCAS 3498 promoter (e.g., SEQ ID NO: 28): or (23) about 10 contiguous nucleic acids up to about 800 contiguous nucleic acids for the CBCAS 3498′ promoter (e.g., SEQ ID NO: 29). In yet other embodiments, the biologically active fragment of the trichome promoter can be any value of contiguous nucleic acids in between these two amounts, such as but not limited to about 20, about 30, about 40, about 50, about 60, about 70, about 80, about 90, about 100, about 110, about 120, about 130, about 140, about 150, about 160, about 170, about 180, about 190, about 200, about 250, about 300, about 350, about 400, about 450, about 500, about 550, about 60, about 650, about 700, about 750, about 800, about 850, about 900, about 950, about 1000, about 1050, about 1100, about 1150, about 1200, about 1250, or about 1300 contiguous nucleic acids. 
     III. Genetic Engineering of Host Cells and Organisms Using Trichome Specific Promoters 
     A. Trichome Specific Promoters 
     The disclosure of the present technology relates to the identification of twenty-three promoters, which are capable of regulating transcription of coding nucleic acid sequences operably linked thereto in trichome cells. 
     Accordingly, the present technology provides an isolated polynucleotide having a nucleic acid sequence that is at least about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, or 100% identical to a nucleic acid sequence described in any of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, wherein the nucleic acid sequence is capable of regulating transcription of coding nucleic acid sequences operably linked thereto in trichome cells. Differences between two nucleic acid sequences may occur at the 5′ or 3′ terminal positions of the reference nucleotide sequence or anywhere between those terminal positions, interspersed either individually among nucleotides in the reference sequence or in one or more contiguous groups within the reference sequence. 
     The present technology also includes biologically active “variants” of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, with one or more bases deleted, substituted, inserted, or added, wherein the nucleic acid sequence is capable of regulating transcription of coding nucleic acid sequences operably linked thereto in trichome cells. Variants of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, include nucleic acid sequences comprising at least about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99% or more nucleic acid sequence identity to SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, and which are trichome specific in their activity. 
     In some embodiments of the present technology, the polynucleotides (promoters) are modified to create variations in the molecule sequences such as to enhance their promoting activities, using methods known in the art, such as PCR-based DNA modification, or standard mutagenesis techniques, or by chemically synthesizing the modified polynucleotides. 
     Accordingly, the sequences set forth in SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33 may be truncated or deleted and still retain the capacity of directing the transcription of an operably linked nucleic acid sequence in trichomes. The minimal length of a promoter region can be determined by systematically removing sequences from the 5′ and 3′-ends of the isolated polynucleotide by standard techniques known in the art, including but not limited to removal of restriction enzyme fragments or digestion with nucleases. 
     Trichome specific promoters of the present technology may also be used for expressing a nucleic acid that will decrease or inhibit expression of a native gene in the plant. Such nucleic acids may encode antisense nucleic acids, ribozymes, sense suppression agents, or other products that inhibit expression of a native gene. 
     The trichome specific promoters of the present technology may also be used to express proteins or peptides in “molecular farming” applications. Such proteins or peptides include but are not limited to industrial enzymes, antibodies, therapeutic agents, and nutritional products. 
     In some embodiments, novel hybrid promoters can be designed or engineered by a number of methods. Many promoters contain upstream sequences which activate, enhance, or define the strength and/or specificity of the promoter. See, e.g., Atchison,  Ann. Rev. Cell Biol.  4:127 (1988). T-DNA genes, for example contain “TATA” boxes defining the site of transcription initiation and other upstream elements located upstream of the transcription initiation site modulate transcription levels. 
     B. Cannabinoid on (CANON) Fragment for Trichome Specific Expression 
     In some embodiments, the disclosure of the present technology also relates to the identification of a nucleic acid molecule termed the “Cannabinoid On” or “CANON” fragment that is sufficient for directing trichome specific expression of coding nucleic acid sequences operably linked thereto. 
     The 171-base pair CANON fragment (SEQ ID NO: 31) is shown below in Table 1. The consensus CANON fragment (highlighted) is shown together with the putative TATA box (bold underline), 5′ UTR, and start codon (“atg” in bold underline) as SEQ ID NO: 32 in Table 1. The consensus is derived from the trichome specific promoters from THCA synthases 19603, 1330, and 50320, CBCA synthase 3498, and CBDA synthase 20800. The 171-base pair CANON fragment (SEQ ID NO: 31) is sufficient to direct trichome specific expression in glandular trichomes of tobacco (and  cannabis ). 
     A nucleic acid sequence comprising four copies of the CANON fragment in front of one copy of the minimal promoter (i.e., TATA Box, start of transcription, and first ATG) termed “4×CANON fragment synthetic promoter” is also shown as SEQ ID NO: 33 in 
     
       
         
           
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 The first CANON fragment of SEQ ID NO: 33 is 
               
               
                 shown in bold, followed by a second fragment 
               
               
                 shown in underline, followed by a third fragment 
               
               
                 in bold, and a fourth fragment in underline. 
               
               
                 Table 1. CANON fragment sequences. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Cannabinoid On (“CANON”) fragment (171 bp) 
               
               
                 atgatgccaaactattcaatqtacaatgtacatttatttttaataagggc 
               
               
                 btcctcccactaacaaaggtgcctaatttttgtgaacttttttttaccac 
               
               
                 atgtgactatttaatgactatcaaattataaaatatttaagtcaatttct 
               
               
                 ttgcccccactccaatatataatgt (SEQ ID NO: 31) 
               
               
                   
               
               
                 CANON fragment with putative TATA Box, 
               
               
                 5′ UTR, and start codon (232 bp) 
               
               
                 atgatgccaaactattcaatgtacaatgtacatttatttttaataagggc 
               
               
                 ttcacctaacaaaggtgcctaatttttgtgaacttttttttaccacatgt 
               
               
                 gactatttaatgactatcaaattataaaatatttaagtcaatttctttgc 
               
               
                 ccccactccaatatataatgt   tataaata   ggataattctcaattcatagt 
               
               
                 attcaaaaatcattaggactaaagaaaa   atg    (SEQ ID NO: 32) 
               
               
                   
               
               
                 4 × CANON fragment synthetic promoter 709 bp) 
               
               
                 
                   atgatgecaaactattcaatgtacaatgtacatttatttttaataagggc 
                 
               
               
                 
                   ttcacctaacaaaggtgcctaatttttgtgaacttttttttaccacatgt 
                 
               
               
                 
                   gactatttaatgactatcaaattataaaatatttaagtcaatttctttgc 
                 
               
               
                 
                   ccccactcc 
                   atgatgccaaactattcaatgtacaatgtacatttattttt 
                 
               
               
                 
                   aataagggcttcacctaacaaaggtgcctaatttttgtgaactttttttt 
                 
               
               
                 
                   accacatgtgactatttaatgactatcaaattataaaatatttaagtcaa 
                 
               
               
                 
                   tttctttgcccccactccatgatgccaaactattcaatgtacaatgtaca 
                 
               
               
                 
                   tttatttttaataagggcttca 
                   cctaacaaaggtgcctaatttttgtgaa 
                 
               
               
                 
                   cttttttttaccacatgtgactatttaatgactatcaaattataaaatat 
                 
               
               
                 
                   ttaagtcaatttcttttgcccccactccatgatgccaaactattcaa 
                   tgt 
                 
               
               
                 
                   acaatgtacatttatttttaataagggattcacctaacaaaggtgcctaa 
                 
               
               
                 
                   tttttgtgaacttttttttaccacatgtgactatttaatgactatcaaat 
                 
               
               
                 
                   tataaaatatttaagtcaatttctttgcccccactccaatatataatgtt 
                 
               
               
                   ataaatagctataattctcaattcatagtaattc aaaaatcattaggact 
               
               
                 aaacgaaaaatg (SEQ ID NO: 33) 
               
               
                   
               
            
           
         
       
     
     The CANON fragment is sufficient to direct trichome specific expression in a gain-of-function promoter. Without wishing to be bound by theory, it is believed that the CANON fragment is responsible for the trichome specific expression of a number of cannabinoid biosynthetic enzyme genes including THCA, CBDA, and CBCA synthases. 
     C. Nucleic Acid Constructs 
     In some embodiments, the trichome specific promoter sequences and CANON fragments of the present technology, or biologically active fragments thereof, can be incorporated into nucleic acid constructs, such as expression constructs (i.e., expression vectors), which can be introduced and replicate in a host cell, such as plant trichome cell. Such nucleic acid constructs may include a heterologous nucleic acid operably linked to any of the promoter sequences or CANON fragments of the present technology. Thus, in some embodiments, the present technology provides the use of any of the promoters or CANON fragments set forth in SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or biologically active fragments thereof, for the expression of homologous or heterologous nucleic acid sequences in a recombinant cell or organism, such as a plant cell or plant. In some embodiments, this use comprises operably linking any of the promoters or CANON fragments set forth in SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or biologically active fragments thereof, to a homologous or heterologous nucleic acid sequence to form a nucleic acid construct and transforming a host, such as a plant or plant cell. In some embodiments, various genes that encode enzymes involved in biosynthetic pathways for the production of cannabinoids (e.g., at least one of the nucleic acid sequences set forth in SEQ ID NOs: 4, 7, 10, 15, 20, 27, or 30) can be suitable as transgenes that can be operably linked to a trichome specific promoter or CANON fragment of the present technology. In some embodiments, the nucleic acid constructs of the present technology modulate the expression of one or more proteins that regulate cannabinoid biosynthesis. In some embodiments, the nucleic acid constructs of the present technology can be used to modulate the expression of cannabinoids or other compounds (e.g., terpenes) in trichome cells. 
     In some embodiments, an expression vector comprises a promoter or CANON fragment comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or a biologically active fragment thereof, operably linked to the cDNA encoding a polypeptide, such as one or more of olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic pyrenyltransferase (PT), hexanoyl-CoA synthetase (AEE1-1), CBDA synthase, CBCA synthase, and THCA synthase. In another embodiment, a plant cell line comprises an expression vector comprising a promoter or CANON fragment comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or a biologically active fragment thereof, operably linked to the cDNA encoding a polypeptide, such as one or more of olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic pyrenyltransferase (PT), hexanoyl-CoA synthetase (AEE1-1), CBDA synthase, CBCA synthase, and THCA synthase. In another embodiment, a transgenic plant comprises an expression vector comprising a promoter or CANON fragment comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or a biologically active fragment thereof, operably linked to the cDNA encoding a polypeptide, such as one or more of olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic pyrenyltransferase (PT), hexanoyl-CoA synthetase (AEE1-1), CBDA synthase, CBCA synthase, and THCA synthase. In another embodiment, methods for genetically modulating the production of cannabinoids are provided, comprising: introducing an expression vector comprising a promoter or CANON fragment comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or a biologically active fragment thereof, operably linked to the cDNA encoding a polypeptide, such as one or more of olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic pyrenyltransferase (PT), hexanoyl-CoA synthetase (AEE1-1), CBDA synthase, CBCA synthase, and THCA synthase. 
     In another embodiment, an expression vector comprises one or more promoters or CANON fragments comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or a biologically active fragment thereof, operably linked to cDNA encoding a polypeptide, such as one or more of olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic pyrenyltransferase (PT), hexanoyl-CoA synthetase (AEE1-1), CBDA synthase, CBCA synthase, and THCA synthase. In another embodiment, a plant cell line comprises one or more promoters comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or a biologically active fragment thereof, operably linked to cDNA encoding a polypeptide, such as one or more of olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic pyrenyltransferase (PT), hexanoyl-CoA synthetase (AEE1-1), CBDA synthase, CBCA synthase, and THCA synthase. In another embodiment, a transgenic plant comprises one or more promoters or CANON fragments comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or a biologically active fragment thereof, operably linked to cDNA encoding a polypeptide, such as one or more of olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic pyrenyltransferase (PT), hexanoyl-CoA synthetase (AEE1-1), CBDA synthase, CBCA synthase, and THCA synthase. In another embodiment, methods for genetically modulating the production level of cannabinoids are provided, comprising introducing into a host cell an expression vector comprising one or more promoters or CANON fragments, comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or a biologically active fragment thereof, operably linked to cDNA encoding a polypeptide, such as one or more of olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic pyrenyltransferase (PT), hexanoyl-CoA synthetase (AEE1-1), CBDA synthase, CBCA synthase, and THCA synthase. 
     Constructs may be comprised within a vector, such as an expression vector adapted for expression in an appropriate host (plant) cell. It will be appreciated that any vector which is capable of producing a plant comprising the introduced DNA sequence will be sufficient. 
     Suitable vectors are well known to those skilled in the art and are described in general technical references such as Pouwels et al.,  Cloning Vectors. A Laboratory Manual , Elsevier, Amsterdam (1986). Vectors for plant transformation have been described (see, e.g., Schardl et al.,  Gene  61:1-14 (1987)). In some embodiments, the nucleic acid construct is a plasmid vector, or a binary vector. Examples of suitable vectors include the Ti plasmid vectors. 
     Recombinant nucleic acid constructs (e.g., expression vectors) capable of introducing nucleotide sequences or chimeric genes under the control of a trichome specific regulatory sequence (e.g., promoter, CANON fragment) may be made using standard techniques generally known in the art. To generate a chimeric gene, an expression vector generally comprises, operably linked in the 5′ to 3′ direction, a trichome specific promoter sequence or CANON sequence that directs the transcription of a downstream homologous or heterologous nucleic acid sequence, and optionally followed by a 3′ untranslated nucleic acid region (3′-UTR) that encodes a polyadenylation signal which functions in plant cells to cause the termination of transcription and the addition of polyadenylate nucleotides to the 3′ end of the mRNA encoding the protein. The homologous or heterologous nucleic acid sequence may be a sequence encoding a protein or peptide or it may be a sequence that is transcribed into an active RNA molecule, such as a sense and/or antisense RNA suitable for silencing a gene or gene family in the host cell or organism. Expression vectors also generally contain a selectable marker. Typical 5′ to 3′ regulatory sequences include a transcription initiation site, a ribosome binding site, an RNA processing signal, a transcription termination site, and/or polyadenylation signal. 
     In some embodiments, the expression vectors of the present technology may contain termination sequences, which are positioned downstream of the nucleic acid molecules of the present technology, such that transcription of mRNA is terminated, and polyA sequences added. Exemplary terminators include  Agrobacterium tumefaciens  nopaline synthase terminator (Tnos),  Agrobacterium tumefaciens  mannopine synthase terminator (Tmas), and the CaMV 35S terminator (T35S). Termination regions include the pea ribulose bisphosphate carboxylase small subunit termination region (TrbcS) or the Tnos termination region. The expression vector also may contain enhancers, start codons, splicing signal sequences, and targeting sequences. 
     In some embodiments, the expression vectors of the present technology may contain a selection marker by which transformed cells can be identified in culture. The marker may be associated with the heterologous nucleic acid molecule, i.e., the gene operably linked to a promoter. As used herein, the term “marker” refers to a gene encoding a trait or a phenotype that permits the selection of, or the screening for, a plant or cell containing the marker. In plants, for example, the marker gene will encode antibiotic or herbicide resistance. This allows for selection of transformed cells from among cells that are not transformed or transfected. 
     Examples of suitable selectable markers include but are not limited to adenosine deaminase, dihydrofolate reductase, hygromycin-B-phosphotransferase, thymidine kinase, xanthine-guanine phospho-ribosyltransferase, glyphosate and glufosinate resistance, and amino-glycoside 3′-O-phosphotransferase (kanamycin, neomycin and G418 resistance). These markers may include resistance to G418, hygromycin, bleomycin, kanamycin, and gentamicin. The construct may also contain the selectable marker gene bar that confers resistance to herbicidal phosphinothricin analogs like ammonium gluphosinate. See, e.g., Thompson et al.,  EMBO J.  9:2519-23 (1987)). Other suitable selection markers known in the art may also be used. 
     Visible markers such as green florescent protein (GFP) may be used. Methods for identifying or selecting transformed plants based on the control of cell division have also been described. See, e.g., WO 2000/052168 and WO 2001/059086. 
     Replication sequences, of bacterial or viral origin, may also be included to allow the vector to be cloned in a bacterial or phage host. Preferably, a broad host range prokaryotic origin of replication is used. A selectable marker for bacteria may be included to allow selection of bacterial cells bearing the desired construct. Suitable prokaryotic selectable markers also include resistance to antibiotics such as kanamycin or tetracycline. 
     Other nucleic acid sequences encoding additional functions may also be present in the vector, as is known in the art. For example, when  Agrobacterium  is the host, T-DNA sequences may be included to facilitate the subsequent transfer to and incorporation into plant chromosomes. 
     Whether a nucleic acid sequence of present technology or biologically active fragment thereof is capable of conferring transcription specifically in trichomes and whether the activity is “strong,” can be determined using various methods. Qualitative methods (e.g., histological GUS (β-glucuronidase) staining) are used to determine the spatio-temporal activity of the promoter or CANON fragment (i.e., whether the promoter or CANON fragment is active in a certain tissue or organ (e.g., trichomes, or under certain environmental/developmental conditions). Quantitative methods (e.g., fluorometric GUS assays) also quantify the level of activity compared to controls. Suitable controls include, but are not limited to, plants transformed with empty vectors (negative controls) or transformed with constructs comprising other promoters, such as the  Arabidopsis  CER6 promoter, which is active in the epidermis and trichomes of  Nicotiana tabacum.    
     To test or quantify the activity of a promoter or CANON fragment of the present technology, a nucleic acid sequence as set forth in SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33, or biologically active fragments thereof, may be operably linked to a known nucleic acid sequence (e.g., a reporter gene such as gusA, or any gene encoding a specific protein) and may be used to transform a plant cell using known methods. The activity of the promoter or CANON fragment can, for example, be assayed (and optionally quantified) by detecting the level of RNA transcripts of the downstream nucleic acid sequence in trichome cells by quantitative RT-PCR or other PCR-based methods. Alternatively, the reporter protein or activity of the reporter protein may be assayed and quantified, by, for example a fluorometric GUS assay if the reporter gene is the gus gene. 
     In some embodiments, the promoters of the present technology can be used to drive expression of a heterologous nucleic acid of interest in trichome cells. The heterologous nucleic acid can encode any man-made recombinant or naturally occurring or protein, such as the cannabinoid biosynthetic pathway enzymes olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic prenyltransferase (PT), hexanoyl-CoA synthetase (AAE1-1), CBDA synthase, THCA synthase, or CBCA synthase as set forth in SEQ ID NOs: 4, 7, 10, 15, 20, 27, and 30, respectively. 
     D. Host Plants and Cells and Plant Regeneration 
     The nucleic acid construct of the present technology can be utilized to transform a host cell, such as a plant cell. In some embodiments, the nucleic acid construct of the present technology is used to transform at least a portion of the cells of a plant. These expression vectors can be transiently introduced into host plant cells or stably integrated into the genomes of host plant cells to generate transgenic plants by various methods known to persons skilled in the art. 
     Methods for introducing nucleic acid constructs into a cell or plant are well known in the art. Suitable methods for introducing nucleic acid constructs (e.g., expression vectors) into plant trichomes to generate transgenic plants include, but are not limited to,  Agrobacterium -mediated transformation, particle gun delivery, microinjection, electroporation, polyethylene glycol-assisted protoplast transformation, and liposome-mediated transformation. Methods for transforming dicots primarily use  Agrobacterium tumefaciens.    
       Agrobacterium rhizogenes  may be used to produce transgenic hairy roots cultures of plants, including  cannabis  and tobacco, as described, for example, by Guillon et al.,  Curr. Opin. Plant Biol.  9:341-6 (2006). “Tobacco hairy roots” refers to tobacco roots that have T-DNA from an Ri plasmid of  Agrobacterium rhizogenes  integrated in the genome and grow in culture without supplementation of auxin and other phytohormones. 
     Additionally, plants maybe transformed by  Rhizobium, Sinorhizobium , or  Mesorhizobium  transformation. (Broothaerts et al.,  Nature  433: 629-633 (2005)). 
     After transformation of the plant cells or plant, those plant cells or plants into which the desired DNA has been incorporated may be selected by such methods as antibiotic resistance, herbicide resistance, tolerance to amino-acid analogues or using phenotypic markers. 
     The transgenic plants can be used in a conventional plant breeding scheme, such as crossing, selfing, or backcrossing, to produce additional transgenic plants containing the transgene. 
     Suitable host cells include plant cells. Any plant may be a suitable host, including monocotyledonous plants or dicotyledonous plants, such as, for example, maize/corn ( Zea  species, e.g.,  Z. mays. Z. diploperennis  (chapule),  Zea luxurians  (Guatemalan teosinte),  Zea mays  subsp.  huehuetenangensis  (San Antonio Huista teosinte),  Z. mays  subsp.  mexicana  (Mexican teosinte),  Z. mays  subsp.  parviglumis  (Balsas teosinte),  Z. perennis  (perennial teosinte) and  Z. ramosa , wheat ( Triticum  species), barley (e.g.,  Hordeum vulgare ), oat (e.g.,  Avena sativa ), sorghum ( Sorghum bicolor ), rye ( Secale cereale ), soybean ( Glycine  spp. e.g.,  G. max ), cotton ( Gossypium  species, e.g.,  G. hirsutum. G. barbadense ),  Brassica  spp. (e.g.,  B. napus. B. juncea, B. oleracea, B. rapa , etc.) sunflower ( Helianthus annus ), tobacco ( Nicotiana  species), alfalfa ( Medicago sativa ), rice ( Oryza  species, e.g.,  O. sativa  indica cultivar-group or  japonica  cultivar-group), forage grasses, pearl millet ( Pennisetum  species. e.g.,  P. glaucum ), tree species, vegetable species, such as  Lycopersicon  ssp (recently reclassified as belonging to the genus  Solanum ), e.g., tomato ( L. esculentum , syn.  Solanum lycopersicum ) such as e.g., cherry tomato, var.  cerasiforme  or current tomato, var.  pimpinellifolium ) or tree tomato ( S. betaceum , syn.  Cyphomandra betaceae ), potato ( Solanum tuberosum ) and other  Solanum  species, such as eggplant ( Solanum melongena ), pepino ( S. muricatum ), cocona ( S. sessiliflorum ) and naranjilla ( S. quitoense ); peppers ( Capsicum annuum, Capsicum frutescens ), pea (e.g.,  Pisum sativum ), bean (e.g.,  Phaseolus  species), carrot ( Daucus carona ),  Lactuca  species (such as  Lactuca sativa, Lactuca indica, Lactuca perennis ), cucumber ( Cucumis sativus ), melon ( Cucumis melo ), zucchini ( Cucurbita pepo ), squash ( Cucurbita maxima, Cucurbita pepo, Cucurbita mixta ), pumpkin ( Cucurbita pepo ), watermelon ( Citrullus lanatus  syn.  Citrullus vulgaris ), fleshy fruit species (grapes, peaches, plums, strawberry, mango, melon), ornamental species (e.g., Rose,  Petunia, Chrysanthemum , Lily, Tulip,  Gerbera  species), woody trees (e.g., species of  Populus, Salix, Quercus, Eucayptus ), fibre species e.g., flax ( Linum usitatissimum ), and hemp ( Cannabis sativa ). In some embodiments, the plant is  Cannabis sativa . In some embodiments, the plant is  Nicotiana tabacum.    
     Thus, in some embodiments, the present technology contemplates the use of the trichome specific promoters and/or CANON fragments comprising the nucleic acid sequences set forth SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, and 31-33, or biologically active fragments thereof, to genetically manipulate the synthesis of cannabinoids (e.g., THC, CBD, CBC) or other molecules in host plants, such as  C. sativa , plants of the family  Solanaceae , such as  N. tabacum , and other plant families and species that do not naturally produce cannabinoids. 
     The present technology also contemplates cell culture systems (e.g., plant cell cultures, bacterial or fungal cell cultures, human or mammalian cell cultures, insect cell cultures) comprising genetically engineered cells transformed with the nucleic acid molecules described herein. In some embodiments, a cell culture comprising cells comprising a promoter or CANON fragment of the present technology is provided. 
     Various assays may be used to determine whether a plant cell shows a change in gene expression, for example, Northern blotting or quantitative reverse transcriptase PCR (RT-PCR). Whole transgenic plants may be regenerated from the transformed cell by conventional methods. Such transgenic plants may be propagated and self-pollinated to produce homozygous lines. Such plants produce seeds containing the genes for the introduced trait and can be grown to produce plants that will produce the selected phenotype. 
     To enhance the expression and/or accumulation of a molecule of interest in trichome cells and/or to facilitate purification of the molecule from trichome cells, methods to down-regulate at least one molecule endogenous to the plant trichomes can be employed. Trichomes are known to contain a number of compounds and metabolites that interfere with the production of other molecules in the trichome cells. These compounds and metabolites include, for example, proteases, polyphenol oxidase (PPO), polyphenols, ketones, terpenoids, and alkaloids. The down-regulation of such trichome components has been described. See, e.g., U.S. Pat. No. 7,498,428. 
     EXAMPLES 
     The following examples are provided by way of illustration only and not by way of limitation. Those of skill in the art will readily recognize a variety of non-critical parameters that could be changed or modified to yield essentially the same or similar results. The examples should in no way be construed as limiting the scope of the present technology, as defined by the appended claims. 
     Example 1: Identifying Trichome Specific Promoters 
     The nucleic acid sequences of the (i) olivetol synthase (OLS) promoter, (ii) OLS1 promoter, and (iii) OLS2 promoter are set forth in SEQ ID NOs: 1, 2, and 3, respectively, and the open reading frame (ORF) of OLS is set forth in SEQ ID NO: 4. The nucleic acid sequence of the olivetolic acid cyclase (OAC) promoter is set forth in SEQ ID NO: 5, the nucleic acid sequence of the OAC1 promoter is set forth in SEQ ID NO: 6, and the ORF of OAC is set forth in SEQ ID NO: 7. The nucleic acid sequence of aromatic prenyltransferase (PT) promoter is set forth in SEQ ID NO: 8, the nucleic acid sequence of the PT1 promoter is set forth in SEQ ID NO: 9, and the ORF of PT is set forth in SEQ ID NO: 10. The nucleic acid sequences of the (i) hexanoyl-CoA synthetase (AAE1-1) promoter, (ii) hexanoyl-CoA synthetase (AAE1-1′) promoter; (iii) hexanoyl-CoA synthetase (AAE3) promoter, and (iv) hexanoyl-CoA synthetase (AAE12) promoter are set forth in SEQ ID NOs: 11, 12, 13, and 14, respectively, and the ORF of hexanoyl-CoA (AAE-1) is set forth in SEQ ID NO: 15. The nucleic acid sequences of the (i) CBDA synthase (CBDAS) promoter, (ii) CBDAS synthase 1 (CBDAS1) promoter, (iii) CBDA synthase (CBDAS) 20800 promoter, and (iv) CBDA synthase (CBDAS) 20800′ promoter are set forth in SEQ ID NOs: 16, 17, 18, and 19, respectively, and the nucleic acid sequence of the ORF of CBDA synthase (CBDAS) is set forth in SEQ ID NO: 20. The nucleic acid sequences of (i) THCA synthase (THCAS) 19603 promoter, (ii) THCA synthase (THCAS) 19603′ promoter, (iii) THCA synthase (THCAS) 50320 promoter, (iv) THCA synthase (THCAS) 50320′ promoter, (v) THCA synthase (THCAS) 1330 promoter, and (vi) THCA synthase (THCAS) 1330′ promoter are set forth in SEQ ID NOs: 21, 22, 23, 24, 25, and 26, respectively, and the ORF of THCAS is set forth in SEQ ID NO: 27. The nucleic acid sequence of the CBCA synthase (CBCAS) 3498 promoter is set forth in SEQ ID NO: 28, the nucleic acid sequence of the CBCA synthase (CBCAS) 3498′ promoter is set forth in SEQ ID NO: 29, and the ORF of CBCA synthase (CBCAS) is set forth in SEQ ID NO: 30. 
     Trichome specific promoters were identified by searching the draft genome sequence of  Cannabis sativa  using the BLAT search facility with the coding regions of the biosynthetic enzyme genes in the cannabinoid biosynthetic pathway. For each genomic sequence hit, a gene prediction program was run (using the FGENESH program) to establish the first ATG. The start of transcription was then established by comparing the genomic sequence to the longest available cDNA sequences in the NCBI NR database. Both multiple sequence alignments (for the CBDA and THCA synthase genes) and querying the PLACE database established TATA Box regions. With the start codon, start of transcription, and TATA Box established, the location and sequence of the promoters was verified. 
     Example 2: Trichome Specific Promoters for Directing Cannabinoid Production in  Nicotiana tabacum    
     Cannabinoids are synthesized and accumulated in  cannabis  trichomes. Olivetol synthase (OLS), olivetolic acid cyclase (OAC), aromatic prenyltransferase (PT), hexanoyl-CoA synthetase (AAE1-1), CBDA synthase, CBCA synthase, and THCA synthase are enzymes of the cannabinoid biosynthetic pathway. Accordingly, it is expected that the promoters for each of these enzymes will direct the expression of coding nucleic acids in trichome cells. This example demonstrates the use of the trichome specific promoters of the present technology, or biologically active fragments thereof, to express cannabinoid biosynthetic enzymes in plants and plant cells that do not naturally produce cannabinoids. 
     Methods 
     Vector constructs. Promoter sequences (SEQ ID NOs: 1-3, 5, 6, 8, 9, 11-14, 16-19, 21-26, 28, 29, or 31-33) are placed in front of a GUS-A marker in a vector adapted for expression in a  Nicotiana tabacum  cell, such as a Ti plasmid vector. The constructs are incorporated into  Agrobacterium tumafaciens  and used to transform  N. tabacum  according to methods known in the art. Constructs are transformed and regenerated under kanamycin selection and primary regenerants (T 0 ) are grown to seed. 
     As a control, a construct containing the tobacco NtCPS2 promoter is transformed into tobacco. The NtCPS2 promoter has been shown to be highly effective in directing trichome-specific expression in  N. tabacum  (Sallaud et al.,  The Plant Journal  72:1-17 (2012)). 
     Expression analysis. Quantitative and qualitative β-glucuronidase (GUS) activity analyses are performed on T 1  plants. Qualitative analysis of promoter activity is carrier out using histological GUS assays and by visualization of the Green Fluorescent Protein (GFP) using a fluorescence microscope. For GUS assays, various plant parts are incubated overnight at 37° C. in the presence of atmospheric oxygen with Xglue (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide cyclohexylamine salt) substrate in phosphate buffer (1 mg/mL, K 2 HPO 4 , 10 μM, pH 7.2, 0.2% Triton X-100). The samples are de-stained by repeated washing with ethanol. Non-transgenic plants are used as negative controls. It is anticipated that trichomes of transgenic plants with OLS:GUS, OAC:GUS, PT:GUS, AAEE1-1:GUS, CBDAS:GUS, CBCAS:GUS, and THCAS:GUS will show bright blue trichomes whereas the non-trichome tissues of these transgenic plants and the trichomes of non-transgenic control plants will not be colored. 
     Quantitative analysis of promoter activity is carried out using a fluorometric GUS assay. Total protein samples are prepared from young leaf material: samples are prepared from pooled leaf pieces. Fresh leaf material is ground in PBS using metal beads followed by centrifugation and collection of the supernatant. 
     Results 
     Plants and plant cells genetically engineered with expression vectors comprising the promoters of the present technology, or biologically active fragments thereof, exhibit trichome specific transcriptional activity. As shown in  FIG.  2 B , trichomes of a transgenic plant transformed with CBDAS 20800:GUS show bright blue trichomes whereas the non-trichome tissues of the plant are not colored. Trichome specific expression for the other promoters in the cannabinoid biosynthetic pathway are qualitatively similar ( FIG.  3   ). Results shown in  FIG.  3    demonstrate the trichome specific expression of promoters from the complete cannabinoid biosynthetic pathway in tobacco trichomes. Accordingly, these results demonstrate that the promoters from the complete cannabinoid biosynthetic pathway as described herein are useful for preferentially directing expression of an operably linked gene in trichome tissue, as compared to expression in the root, leaf, stem, or other tissues of a plant. This trichome-specific expression will be a crucial tool for the manipulation of the biosynthesis of trichome-specific biochemical compounds such as the cytotoxic cannabinoids. In addition. these promoters will be crucial to strategies aimed at using tobacco or  cannabis  trichomes as biofactories for the controlled production of specific biochemical compounds. 
     Example 3: Identifying “Cannabinoid On” (“CANON”) Fragments for Directing Trichome Specific Expression in  Nicotiana Tabacum    
     The nucleic acid sequence of the “Cannabinoid On” (“CANON”) fragment is set forth in SEQ ID NO: 31. The nucleic acid sequence of the CANON fragment together with the putative TATA Box, 5′ UTR, and start codon is set forth in SEQ ID NO: 32. The nucleic acid sequence of the 4×CANON fragment synthetic promoter is set forth in SEQ ID NO: 33. The consensus CANON fragment is derived from the trichome specific promoters from THCA synthases 19603, 1330, 50320, CBCA synthase 3498, and CBDA synthase 20800. 
     Methods 
     CANON fragments were identified by searching the purple Kush genome sequence using the BLAT search facility. A BLAT search of the purple Kush genome sequence using a THCA synthase gene yielded the following results: 
     
       
         
           
               
             
               
                   
               
               
                 BLAT Search Results¶ 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 •••ACTIONS••••••QUERY•••••••••••SCORE•START••END•QSIZE•IDENTITY•CHRO•STRAND••START••••END••••••SPAN¶ 
               
               
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                 browser•details•THCA•••••••••••••979•••••1•••979•••979•100.0%•• scaffold19603 •••+•••••••6901••••••7879••••979•¶ 
               
               
                 browser•details•THCA•••••••••••••499•••257•••979•••979••89.4%•• scaffold50320 •••−•••••••8585••••••9462••••878¶ 
               
               
                 browser•details•THCA•••••••••••••466•••257•••979•••979••89.1%•• scaffold1330 •••−•••••••2572••••••3369••••798¶ 
               
               
                 browser•details•THCA•••••••••••••332•••593•••979•••979••94.2%•• scaffold3498 •••+•••••••9881•••••10270••••390¶ 
               
               
                 browser•details•THCA•••••••••••••275•••590•••979•••979••85.6%••scaffold6274•••+••••••24419•••••24803••••385¶ 
               
               
                 browser•details•THCA•••••••••••••271•••590•••979•••979••96.5%••scaffold39155•••−•••••••4079••••••4464••••386¶ 
               
               
                 browser•details•THCA•••••••••••••251•••••1•••258•••979••97.7%••scaffold17297•••+•••••••9858•••••10113••••256¶ 
               
               
                 browser•details•THCA•••••••••••••222•••590•••979•••979••96.0%••scaffold46100•••−•••••••1795••••••2180••••386¶ 
               
               
                 browser•details•THCA•••••••••••••206•••••3•••271•••979••89.0%••scaffold33063•••−••••••10241•••••10479••••239¶ 
               
               
                 browser•details•THCA•••••••••••••196•••578•••979•••979••91.2%•• scaffold20800 •••−•••••••4646••••••5042••••397¶ 
               
               
                 browser•details•THCA•••••••••••••167••••69•••267•••979••94.7%••scaffold12036•••+•••••133813••••134227••••415¶ 
               
               
                 browser•details•THCA•••••••••••••156••••89•••258•••979••96.5%••scaffold17775•••−••••••18105•••••18274••••170¶ 
               
               
                 browser•details•THCA•••••••••••••142•••260•••514•••979••87.5%••scaffold59317•••+••••••••863••••••1300••••438¶ 
               
               
                 browser•details•THCA•••••••••••••136•••120•••268•••979••96.6%••scaffold12751•••−••••••46584•••••46732••••149¶ 
               
               
                 browser•details•THCA•••••••••••••128•••120•••257•••979••97.1%••scaffold87266•••−••••••12096•••••12233••••138¶ 
               
               
                 browser•details•THCA•••••••••••••128•••120•••257•••979••97.1%••scaffold23985•••+••••••18853•••••18990••••138¶ 
               
               
                 browser•details•THCA•••••••••••••127•••120•••258•••979••96.4%••scaffold14882•••+••••••59722•••••59860••••139¶ 
               
               
                 browser•details•THCA•••••••••••••123••••98•••269•••979••85.9%••scaffold4283•••−••••••23256•••••23416••••161¶ 
               
               
                   
               
            
           
         
       
     
     The bolded promoters were selected for further analysis and the sequences of the five promoters were compared. The five promoters showed high sequence similarity from the start of translation to about 160 base pairs upstream of the putative TATA Box, but little sequence similarity upstream of the TATA Box. 
     A consensus sequence was derived from the five promoters and the sequence was used to test for trichome specific promoter activity in tobacco trichomes using a synthetic promoter termed the “4×CANON fragment synthetic promoter.” The 4×CANON fragment synthetic promoter comprises four copies of the consensus CANON fragment, which is not found in nature, in front of one copy of the minimal promoter (TATA Box, start of transcription and first ATG).  N. tabacum  was transformed with the 4×CANON fragment synthetic promoter placed in front of a GUS-A marker as described in Example 2 and GUS expression analyses were performed. 
     Results 
     As shown in  FIG.  4 B , the 4×CANON fragment synthetic promoter directs expression in the trichome. Accordingly, these results demonstrate that the CANON fragment is sufficient to direct trichome specific expression in a gain-of-function promoter and is therefore responsible for the trichome specific expression of a number of cannabinoid biosynthetic enzyme genes including THCA, CBDA, and CBCA synthases. 
     EQUIVALENTS 
     The present technology is not to be limited in terms of the particular embodiments described in this application, which are intended as single illustrations of individual aspects of the present technology. Many modifications and variations of this present technology can be made without departing from its spirit and scope, as will be apparent to those skilled in the art. Functionally equivalent methods and apparatuses within the scope of the present technology, in addition to those enumerated herein, will be apparent to those skilled in the art from the foregoing descriptions. Such modifications and variations are intended to fall within the scope of the appended claims. The present technology is to be limited only by the terms of the appended claims, along with the full scope of equivalents to which such claims are entitled. It is to be understood that this present technology is not limited to particular methods, reagents, compounds compositions or biological systems, which can, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting. 
     In addition, where features or aspects of the disclosure are described in terms of Markush groups, those skilled in the art will recognize that the disclosure is also thereby described in terms of any individual member or subgroup of members of the Markush group. 
     As will be understood by one skilled in the art, for any and all purposes, particularly in terms of providing a written description, all ranges disclosed herein also encompass any and all possible subranges and combinations of subranges thereof. Any listed range can be easily recognized as sufficiently describing and enabling the same range being broken down into at least equal halves, thirds, quarters, fifths, tenths, etc. As a non-limiting example, each range discussed herein can be readily broken down into a lower third, middle third and upper third, etc. As will also be understood by one skilled in the art all language such as “up to,” “at least,” “greater than,” “less than,” and the like, include the number recited and refer to ranges which can be subsequently broken down into subranges as discussed above. Finally, as will be understood by one skilled in the art, a range includes each individual member. Thus, for example, a group having 1-3 cells refers to groups having 1, 2, or 3 cells. Similarly. a group having 1-5 cells refers to groups having 1, 2, 3, 4, or 5 cells, and so forth. 
     All publicly available documents referenced or cited to herein, such as patents, patent applications, provisional applications, and publications, including GenBank Accession Numbers, are incorporated by reference in their entirety, including all figures and tables, to the extent they are not inconsistent with the explicit teachings of this specification. 
     Other embodiments are set forth within the following claims. 
     
       
         
           
               
             
               
                   
               
               
                 SEQUENCE LISTING 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 (554 bp) 
               
               
                 Tetraketide synthase/olivetol synthase (OLS) promoter 
               
               
                 SEQ ID NO: 1 
               
               
                 AAAAATAAAATTAATAAATTTTTAATTATTAATATCATTTTATTTTTTAA 
               
               
                   
               
               
                 TAAATAAATATCATTTTTATATTATTATAATATGTATAAAGTTTTAATTG 
               
               
                   
               
               
                 TATACAAGAAGTCTTATAGTAAGAGTATACACCTTACATCATAATAACTA 
               
               
                   
               
               
                 CTCGATCTGAAATCAATGGTCAAGAAAAGTTCCCTACCGGTAGGAAACTT 
               
               
                   
               
               
                 TTGCTAGATCCTACCATAGTCTTCCCTTATATTTATTATGTAGAATCTAT 
               
               
                   
               
               
                 TATTATATCTAATAATTAACAAATATTAACAAATCATTTTGAAAAATTAT 
               
               
                   
               
               
                 ATTAAAAAAAAAACTTGAAAAGTCAAAGATTAACCATCAATTTGCCAAAT 
               
               
                   
               
               
                 CAAATTAGTGAGAAAGTAGGTATTATATACCTAACACTCATTTTACATAT 
               
               
                   
               
               
                 GTCTAGACGTTTATATGTATAGCGTTGTTGTGTGTAATAAGTTCACTTGT 
               
               
                   
               
               
                 AGTATCTTGTACATACATAATATATATATATAGGTGTGTTTGTGAACAAT 
               
               
                   
               
               
                 TATATTATCACACATACACAACTCATTATTATCATAATAATAATAATACC 
               
               
                   
               
               
                 ATGG 
               
               
                   
               
               
                 (550 bp) 
               
               
                 Tetraketide synthase/olivetol synthase 1 (OLS1) promoter 
               
               
                 SEQ ID NO: 2  
               
               
                 AAAAATAAAATTAATAAATTTTTAATTATTAATATCATTTTATTTTTTAA 
               
               
                   
               
               
                 TAAATAAATATCATTTTTATATTATTATAATATGTATAAAGTTTTAATTG 
               
               
                   
               
               
                 TATACAAGAAGTCTTATAGTAAGAGTATACACCTTACATCATAATAACTA 
               
               
                   
               
               
                 CTCGATCTGAAATCAATGGTCAAGAAAAGTTCCCTACCGGTAGGAAACTT 
               
               
                   
               
               
                 TTGCTAGATCCTACCATAGTCTTCCCTTATATTTATTATGTAGGATCTAT 
               
               
                   
               
               
                 TATTATATCTAATAATTAACAAATATTAACAAATCATTTTGAAAAATTAT 
               
               
                   
               
               
                 ATTAAAAAAAAAACTTGAAAAGTCAAAGATTAACCATCAATTTGCCAAAT 
               
               
                   
               
               
                 CAAATTAGTGAGAAAGTAGGTATTATATACCTAACACTCATTTTACATAT 
               
               
                   
               
               
                 GTCTAGACGTTTATATGTATAGCGTTGTTGTGTGTAATAAGTTCACTTGT 
               
               
                   
               
               
                 AGTATCTTGTACATACATAATATATATATATAGGTGTGTTTGTGAACAAT 
               
               
                   
               
               
                 TATATTATCACACATACACAACTCATTATTATCATAATAATAATAATAAT 
               
               
                   
               
               
                 (558 bp) 
               
               
                 Tetraketide synthase/olivetol synthase 2 (OLS2) promoter 
               
               
                 SEQ ID NO: 3 
               
               
                 AAAAATAAAATTAATAAATTTTTAATTATTAATATCATTTTATTTTTTAA 
               
               
                   
               
               
                 TAAATAAATATCATTTTTATATTATTATAATATGTATAAAGTTTTAATTG 
               
               
                   
               
               
                 TATACAAGAAGTCTTATAGTAAGAGTATACACCTTACATCATAATAACTA 
               
               
                   
               
               
                 CTCGATCTGAAATCAATGGTCAAGAAAAGTTCCCTACCGGTAGGAAACTT 
               
               
                   
               
               
                 TTGCTAGATCCTACCATAGTCTTCCCTTATATTTATTATGTAGAATCTAT 
               
               
                   
               
               
                 TATTATATCTAATAATTAACAAATATTAACAAATCATTTTGAAAAATTAT 
               
               
                   
               
               
                 ATTAAAAAAAAAACTTGAAAAGTCAAAGATTAACCATCAATTTGCCAAAT 
               
               
                   
               
               
                 CAAATTAGTGAGAAAGTAGGTATTATATACCTAACACTCATTTTACATAT 
               
               
                   
               
               
                 GTCTAGACGTTTATATGTATAGCGTTGTTGTGTGTAATAAGTTCACTTGT 
               
               
                   
               
               
                 AGTATCTTGTACATACATAATATATATATATATAGGTGTGTTTGTGAACA 
               
               
                   
               
               
                 ATTATAATATCACACATACACAACTCATTATTATTAGCATAATAATAATA 
               
               
                   
               
               
                 ATAATAAT 
               
               
                   
               
               
                 (1158 bp) 
               
               
                 Olivetol synthase (OLS) ORF 
               
               
                 SEQ ID NO: 4 
               
            
           
           
               
               
            
               
                 1 
                 atgaatcatc ttcgtgctga gggtccggcc tccgttctcg ccattggcac cgccaatccg 
               
               
                   
               
               
                 61 
                 gagaacattt tattacaaga tgagtttcct gactactatt ttcgcgtcac caaaagtgaa 
               
               
                   
               
               
                 121 
                 cacatgactc aactcaaaga aaagtttcga aaaatatgtg acaaaagtat gataaggaaa 
               
               
                   
               
               
                 181 
                 cgtaactgtt tcttaaatga agaacaccta aagcaaaacc caagattggt ggagcacgag 
               
               
                   
               
               
                 241 
                 atgcaaactc tggatgcacg tcaagacatg ttggtagttg aggttccaaa acttgggaag 
               
               
                   
               
               
                 301 
                 gatgcttgtg caaacmccat caaagaatgg ggtcaaccca agtctaaaat cactcattta 
               
               
                   
               
               
                 361 
                 atcttcacta gcgcatcaac cactgacatg cccggtgcag actaccattg cgctaagctt 
               
               
                   
               
               
                 421 
                 ctcggactga gtccctcagt gaagcgtgtg atgatgtatc aactaggctg ttatggtggt 
               
               
                   
               
               
                 481 
                 ggaaccgttc tacgcattgc caaggacata gcagagaata acaaaggcgc acgagttctc 
               
               
                   
               
               
                 541 
                 gccgtgtgtt gtgacataat ggcttgcttg tttcgtgggc cttcagagtc tgacctcgaa 
               
               
                   
               
               
                 601 
                 ttactagtgg gacaagctat ctttggtgat ggggctgctg cggtgattgt tggagctgaa 
               
               
                   
               
               
                 661 
                 cccgatgagt cagttgggga aaggccgata tttgagttgg tgtcaactgg gcaaacaatc 
               
               
                   
               
               
                 721 
                 ttaccaaact cggaaggaac tattggggga catataaggg aagcaggact gatatttgat 
               
               
                   
               
               
                 781 
                 ttacataagg atgtgcctat gttgatctct aataatattg agaaatgttt gattgaggca 
               
               
                   
               
               
                 841 
                 tttactccta ttgggattag tgattggaac tccatatttt ggattacaca cccaggtggg 
               
               
                   
               
               
                 901 
                 aaagctattt tggacaaagt ggaggagaag ttgcatctaa agagtgataa gtttgtggat 
               
               
                   
               
               
                 961 
                 tcacgtcatg tgctgagtga gcatgggaat atgtctagct caactgtctt gtttgttatg 
               
               
                   
               
               
                 1021 
                 gatgagttga ggaagaggtc gttggaggaa gggaagtcta ccactggaga tggatttgag 
               
               
                   
               
               
                 1081 
                 tggggtgttc tttttgggtt tggaccaggt ttgactgtcg aaagagtggt cgtgcgtagt 
               
               
                   
               
               
                 1141 
                 gttcccatca aatattaa 
               
               
                   
               
            
           
           
               
            
               
                 (996 bp) 
               
               
                 Olivelotic acid cyclase (OAC) promoter 
               
               
                 SEQ ID NO: 5 
               
               
                 AATTTTTGACAATTTTTTTTAATATAACTAACTTGAAGATAATTCCTAAT 
               
               
                   
               
               
                 ACGAATAAATACAGAAAATATAAACAGTTTTGTTATAACACTTTTAGATC 
               
               
                   
               
               
                 AGATTATAATTAAATTTTATATTTTTGATAAAAAAATCAATTGAGGGTCC 
               
               
                   
               
               
                 TATTTGTACCATTTTAGAAAATATAGTGTCCATTTTTATTATTTAGTAAA 
               
               
                   
               
               
                 ACAGAGAGTCTAATGCGTAACTTTTACAAAAATACATGGTCCAAAATAGT 
               
               
                   
               
               
                 ATTTACCCTTTAATTTTATTAGCAACTGTCCCAAAAAAAATATGTTTTGA 
               
               
                   
               
               
                 TGACTGACGTACGGGAATGTAAAGTTTTGTAATAGTGTATTGATTCAAAG 
               
               
                   
               
               
                 ACAACATAAAGACATCAATCTGAAATCGATTTCAATGTCCCAAAAACACA 
               
               
                   
               
               
                 TTAATGAGCCTTAATTGCATCCGATAATCATTTTCCATTGGTTTTATTAC 
               
               
                   
               
               
                 TTCTCATATATATAGACATCACATATATGATAGGATTTCTTGAGAATAAT 
               
               
                   
               
               
                 GTTAACATTGAGATTTTTATTACAACTGATATATTGATTATGCTTAGATG 
               
               
                   
               
               
                 GCTTGAATTTGAGCGACATATATAGAAAGAGTATAGAATGATATATATGC 
               
               
                   
               
               
                 ACATCCAAAATATGTACCAAAATATTAGGTGACAATTTAGATAAATGGAA 
               
               
                   
               
               
                 GAGAAAATTATAGAAAAATGAGGGTTTATATTTTTGTGGTTATTTAATTT 
               
               
                   
               
               
                 TAGACATAGTGTGATCCAATAATTTTAGGATGTATAATTGTTAGGCACAT 
               
               
                   
               
               
                 GGATTACTTGTTTTTTTATTAAGTATAACCTTACAAAGTAGATGGTAGTA 
               
               
                   
               
               
                 ATTAATGTAGAAGGTTCCAATAATGTATTTATATAAATTGTTAGGCATGC 
               
               
                   
               
               
                 AAAGCCTAATTAATTAATAAATGAGGGTGGCCAATGGCCACTATATATAT 
               
               
                   
               
               
                 CAAGGCATCGACTGTATGTAGCATAATGTGATTTATATAATTATCAAAAA 
               
               
                   
               
               
                 AAAAAAATAAAAATAAGAAGAAGAAGAAAGTTGAGAAAGACCATGG 
               
               
                   
               
               
                 (992 bp) 
               
               
                 Olivetolic acid cyclase 1 (OAC1) promoter 
               
               
                 SEQ ID NO: 6 
               
               
                 AATTTTTGACAATTTTTTTTAATATAACTAACTTGAAGATAATTCCTAAT 
               
               
                   
               
               
                 ACGAATAAATACAGAAAATATAAACAGTTTTGTTATAACACTTTTAGATC 
               
               
                   
               
               
                 AGATTATAATTAAATTTTATATTTTTGATAAAAAAATCAATTGAGGGTCC 
               
               
                   
               
               
                 TATTTGTACCATTTTAGAAAATATAGTGTCCATTTTTATTATTTAGTAAA 
               
               
                   
               
               
                 ACAGAGAGTCTAATGCGTAACTTTTACAAAAATACATGGTCCAAAATAGT 
               
               
                   
               
               
                 ATTTACCCTTTAATTTTATTAGCAACTGTCCCAAAAAAAATATGTTTTGA 
               
               
                   
               
               
                 TGACTGACGTACGGGAATGTAAAGTTTTGTAATAGTGTATTGATTCAAAG 
               
               
                   
               
               
                 ACAACATAAAGACATCAATCTGAAATCGATTTCAATGTCCCAAAAACACA 
               
               
                   
               
               
                 TTAATGAGCCTTAATTGCATCCGATAATCATTTTCCATTGGTTTTATTAC 
               
               
                   
               
               
                 TTCTCATATATATAGACATCACATATATGATAGGATTTCTTGAGAATAAT 
               
               
                   
               
               
                 GTTAACATTGAGATTTTTATTACAACTGATATATTGATTATGCTTAGATG 
               
               
                   
               
               
                 GCTTGAATTTGAGCGACATATATAGAAAGAGTATAGAATGATATATATGC 
               
               
                   
               
               
                 ACATCCAAAATATGTACCAAAATATTAGGTGACAATTTAGATAAATGGAA 
               
               
                   
               
               
                 GAGAAAATTATAGAAAAATGAAAATTTATATTTTTGTGGTTATTTAATTT 
               
               
                   
               
               
                 TAGACATAGTGTGATCCAATAATTTTAGGATGTATAATTGTTAGGCACAT 
               
               
                   
               
               
                 GGATTACTTGTTTTTTTATTAAGTATAACCTTACAAAGTAGATGGTAGTA 
               
               
                   
               
               
                 ATTAATGTAGAAGGTTCCAATAATGTATTTATATAAATTGTTAGGCATGC 
               
               
                   
               
               
                 AAAGCCTAATTAATTAATAAATGAGGGTGGCCAATGGCCACTATATATAT 
               
               
                   
               
               
                 CAAGGCATCGACTGTATGTAGCATAATGTGATTTATATAATTATCAAAAA 
               
               
                   
               
               
                 AAAAAAATAAAAATAAGAAGAAGAAGAAAGTTGAGAAAGAGA 
               
               
                   
               
               
                 (485 bp) 
               
               
                 OlivetoLic acid cyclase (OAC) ORF 
               
               
                 SEQ ID NO: 7 
               
            
           
           
               
               
            
               
                 1 
                 aaaaaagaag aagaagaaga aagttgagaa agagaatggc agtgaagcat ttgattgtat 
               
               
                   
               
               
                 61 
                 tgaagttcaa agatgaaatc acagaagccc aaaaggaaga atttttcaag acgtatgtga 
               
               
                   
               
               
                 121 
                 atcttgtgaa tatcatccca gccatgaaag atgtatactg gggtaaagat gtgactcaaa 
               
               
                   
               
               
                 181 
                 agaataagga agaagggtac actcacatag ttgaggtaac atttgagagt gtggagacta 
               
               
                   
               
               
                 241 
                 ttcaggacta cattattcat cctgcccatg ttggatttgg agatgtctat cgttctttct 
               
               
                   
               
               
                 301 
                 gggaaaaact tctcattttt gactacacac cacgaaagta gactatatat agtagccgac 
               
               
                   
               
               
                 361 
                 caagctgcct tcatcttcat cttctcaaat aatatatcta atatctaatt atataataat 
               
               
                   
               
               
                 421 
                 aactacttaa taaaagactg tgtttataac attaaataat aataataata aagtcttttg 
               
               
                   
               
               
                 481 
                 tagct 
               
               
                   
               
            
           
           
               
            
               
                 (1361 bp) 
               
               
                 Aromatic prenyltransferase (PT) promoter 
               
               
                 SEQ ID NO: 8 
               
               
                 TCAAACCAGATTTTTTATAATAATTT 
               
               
                   
               
               
                 ACACACCAATTAACTAAGAAATTCTAACTAGAGTTAGTGTTGAATTAGAA 
               
               
                   
               
               
                 GGAGTATCAAGGTAACCTATCTCCTTTGTCTCTTTAAATTTTTAAAAGAA 
               
               
                   
               
               
                 AAGCTaTTGTTGTAATTAGTTTGGATTTTATGGGTTAGGTTTTGTAGAGT 
               
               
                   
               
               
                 TTGTTTGAGCTTAAAATTGTTGTTGGTTTGATTTAAGGTTGAATTCTGTG 
               
               
                   
               
               
                 TTGGTTTGTAAGATTTTAGCTGATGTAGTTCGTGTTGGAGGAGTTCGAGT 
               
               
                   
               
               
                 TTTTGAACTCAAAGTCCACTCAACAATATGGTGAAATTAGTTTTAGGTGG 
               
               
                   
               
               
                 TTTTAGATGCTTTTGAAGTGTATGTTTTGTTTAGATTACCCTCTTTTTGG 
               
               
                   
               
               
                 TAAGTTTGATAGCCTTTGGTCAAGGAATGACTTAATTTTGAAGGTCTGAA 
               
               
                   
               
               
                 AGTTGAGTAGTTTTGAACTCTTTTCTGCCCAAAATGGTTTCGAATGAGTT 
               
               
                   
               
               
                 GTACTTGTCATATGTGATTTGAGTGGTAAGGTTXGGAAGGGTAGTCCTAG 
               
               
                   
               
               
                 ACAGTGTTTTCAATGATTAGGTTTGGGGTTGGTGAATCTAGAGTTAAGAG 
               
               
                   
               
               
                 CCTAGAGTTTTGGTTGCTTGGATTGGTGTGACCTGGTTGTTAGTATCCTT 
               
               
                   
               
               
                 ATCAAGGGATCCCGAATAGGTGATGAGTGAGGTTTGGATGGTATATTCTG 
               
               
                   
               
               
                 GATCAAAATTTTTAGATGGGGTXXAAACTGATAGTTTCAAAAATGTGTGT 
               
               
                   
               
               
                 GTGTGTCTGGTTAAGGATTTATTTTGGGAAGGCTCTAGTTGGGAGGTCCT 
               
               
                   
               
               
                 GGATTGGAAGTTCTAGATGTGAGGCTCCGGGTAGGAGGGTCCTGTAGGAA 
               
               
                   
               
               
                 GATTCTAGATGGTATTTGGTGGTCGAGATGAGGGATTTCCGAACATGTTT 
               
               
                   
               
               
                 TAATAGTTACAATAATTCATAAATTTTCAACACTAAATCAAAATATTAAT 
               
               
                   
               
               
                 TTTGATATGATTGTATAAATTTAATCTCATCGTAGTGTAGAGCTCTACAT 
               
               
                   
               
               
                 GCATGCATGGTTTGTATAGCCATAATTAATTAATTAGGCAAGAAAGATAG 
               
               
                   
               
               
                 ATGTGCCTACCATGCATGACTTCTAAAACATGGATGACTTTAAATTTTGA 
               
               
                   
               
               
                 AACCAAGCAAAAATTGTTTTACTATAAATCTTGTTAATTTTTCATTATTG 
               
               
                   
               
               
                 ATATCACTATGTTATTCTAACCCTTTCGTAGCCATAATTAGTTAATTAGG 
               
               
                   
               
               
                 CAAGAAAGATAGATGTGCCTACCATGCATGACTTCTAAAACATGGATGAC 
               
               
                   
               
               
                 TTTAAATTTTGAAACCAAGCAAAAATTATTTTACTATAAATCTTGTTATT 
               
               
                   
               
               
                 TTTTCATTATTGATATCACTATGTTATTCTAACCCTTTCTTTCCCTCATT 
               
               
                   
               
               
                 TTTTCTTAATATTCAATCAATAATAATCTCCATGG 
               
               
                   
               
               
                 (1357 bp) 
               
               
                 Aromatic prenyltransferase 1 (PT1) promoter 
               
               
                 SEQ ID NO: 9 
               
               
                 TCAAACCAGATTTTTTATAATAATTT 
               
               
                   
               
               
                 ACACACCAATTAACTAAGAAATTCTAACTAGAGTTAGTGTTGAATTAGAA 
               
               
                   
               
               
                 GGAGTATCAAGGTAACCTATCTCCTTTGTCTCTTTAAATTTTTAAAAGAA 
               
               
                   
               
               
                 AAGCTaTTGTTGTAATTAGTTTGGATTTTATGGGTTAGGTTTTGTAGAGT 
               
               
                   
               
               
                 TTGTTTGAGCTTAAAATTGTTGTTGGTTTGATTTAAGGTTGAATTCTGTG 
               
               
                   
               
               
                 TTGGTTTGTAAGATTTTAGCTGATGTAGTTCGTGTTGGAGGAGTTCGAGT 
               
               
                   
               
               
                 TTTTGAACTCAAAGTCCACTCAACAATATGGTGAAATTAGTTTTAGGTGG 
               
               
                   
               
               
                 TTTTAGATGCTTTTGAAGTGTATGTTTTGTTTAGATTACCCTCTTTTTGG 
               
               
                   
               
               
                 TAAGTTTGATAGCCTTTGGTCAAGGAATGACTTAATTTTGAAGGTCTGAA 
               
               
                   
               
               
                 AGTTGAGTAGTTTTGAACTCTTTTCTGCCCAAAATGGTTTCGAATGAGTT 
               
               
                   
               
               
                 GTACTTGTCATATGTGATTTGAGTGGTAAGGTTCGGAAGGGTAGTCCTAG 
               
               
                   
               
               
                 ACAGTGTTTTCAATGATTAGGTTTGGGGTTGGTGAATCTAGAGTTAAGAG 
               
               
                   
               
               
                 CCTAGAGTTTTGGTTGCTTGGATTGGTGTGACCTGGTTGTTAGTATCCTT 
               
               
                   
               
               
                 ATCAAGGGATCCCGAATAGGTGATGAGTGAGGTTTGGATGGTATATTCTG 
               
               
                   
               
               
                 GATCAAAATTTTTAGATGGGGTCCAAACTGATAGTTTCAAAAATGTGTGT 
               
               
                   
               
               
                 GTGTGTCTGGTTAAGGATTTATTTTGGGAAGGCTCTAGTTGGGAGGTCCT 
               
               
                   
               
               
                 GGATTGGAAGTTCTAGATGTGAGGCTCCGGGTAGGAGGGTCCTGTAGGAA 
               
               
                   
               
               
                 GATTCTAGATGGTATTTGGTGGTCGAGATGAGGGATTTCCGAACATGTTT 
               
               
                   
               
               
                 TAATAGTTACAATAATTCATAAATTTTCAACACTAAATCAAAATATTAAT 
               
               
                   
               
               
                 TTTGATATGATTGTATAAATTTAATCTCATCGTAGTGTAGAGCTCTACAT 
               
               
                   
               
               
                 GCATGCATGGTTTGTATAGCCATAATTAATTAATTAGGCAAGAAAGATAG 
               
               
                   
               
               
                 ATGTGCCTACCATGCATGACTTCTAAAACATGGATGACTTTAAATTTTGA 
               
               
                   
               
               
                 AACCAAGCAAAAATTGTTTTACTATAAATCTTGTTAATTTTTCATTATTG 
               
               
                   
               
               
                 ATATCACTATGTTATTCTAACCCTTTCGTAGCCATAATTAGTTAATTAGG 
               
               
                   
               
               
                 CAAGAAAGATAGATGTGCCTACCATGCATGACTTCTAAAACATGGATGAC 
               
               
                   
               
               
                 TTTAAATTTTGAAACCAAGCAAAAATTATTTTACTATAAATCTTGTTATT 
               
               
                   
               
               
                 TTTTCATTATTGATATCACTATGTTATTCTAACCCTTTCTTTCCCTCATT 
               
               
                   
               
               
                 TTTTCTTAATATTCAATCAATAATAATCTCC 
               
               
                   
               
               
                 (1188 bp) 
               
               
                 Aromatic prenyltransferase (PT) ORF 
               
               
                 SEQ ID NO: 10 
               
            
           
           
               
               
            
               
                 1 
                 atgggactct catcagtttg taccttttca tttcaaacta attaccatac tttattaaat 
               
               
                   
               
               
                 61 
                 cctcacaata ataatcccaa aacctcatta ttatgttatc gacaccccaa aacaccaatt 
               
               
                   
               
               
                 121 
                 aaatactctt acaataattt tccctctaaa cattgctcca ccaagagttt tcatctacaa 
               
               
                   
               
               
                 181 
                 aacaaatgct cagaatcatt atcaatcgca aaaaattcca ttagggcagc tactacaaat 
               
               
                   
               
               
                 241 
                 caaactgagc ctccagaatc tgataatcat tcagtagcaa ctaaaatttt aaactttggg 
               
               
                   
               
               
                 301 
                 aaggcatgtt ggaaacttca aagaccatat acaatcatag catttacttc atgcgcttgt 
               
               
                   
               
               
                 361 
                 ggattgtttg ggaaagagtt gttgcataac acaaatttaa taagttggtc tctgatgttc 
               
               
                   
               
               
                 421 
                 aaggcattct tttttttggt ggctatatta tgcattgctt cttttacaac taccatcaat 
               
               
                   
               
               
                 481 
                 cagatttacg atcttcacat tgacagaata aacaagcctg atctaccact agettcaggg 
               
               
                   
               
               
                 541 
                 gaaatatcag taaacacagc ttggattatg agcataattg tggcactgtt tggattgata 
               
               
                   
               
               
                 601 
                 ataactataa aaatgaaggg tggaccactc tatatatttg gctactgttt LggLattttt 
               
               
                   
               
               
                 661 
                 ggtgggattg tctattctgt tccaccattt agatggaagc aaaatccttc cactgcattt 
               
               
                   
               
               
                 721 
                 cttctcaatt tcctggccca tattattaca aatttcacat tttattatgc cagcagagca 
               
               
                   
               
               
                 781 
                 gctcttggcc taccatttga gttgaggcct tcttttactt tcctgctagc atttatgaaa 
               
               
                   
               
               
                 841 
                 tcaatgggtt cagctttggc tttaatcaaa gatgcttcag acgttgaagg cgacactaaa 
               
               
                   
               
               
                 901 
                 tttggcatat caaccttggc aagtaaatat ggttccagaa acttgacatt attttgttct 
               
               
                   
               
               
                 961 
                 ggaattgttc tcctatecta tgtggctgct atacttgctg ggattatctg gccccaggct 
               
               
                   
               
               
                 1021 
                 ttcaacagta acgtaatgtt actttctcat gcaatcttag cattttggtt aatcctccag 
               
               
                   
               
               
                 1081 
                 actcgagatt ttgcgttaac aaattacgac ccggaagcag gcagaagatt ttacgagttc 
               
               
                   
               
               
                 1141 
                 atgtggaagc tttattatgc tgaatattta gtatatgttt tcatataa 
               
               
                   
               
            
           
           
               
            
               
                 (805 bp) 
               
               
                 Hexanoyl-CoA synthetase (AAE1-1) promoter 
               
               
                 SEQ ID NO: 11 
               
               
                 ATAATGTTATTTATTATTTAATACAATATGACATTTATACAGCGTACCAA 
               
               
                   
               
               
                 ACGAGCCCATAATGTATTGCAATTGTATTGTTGTACGAATTAAAATAATA 
               
               
                   
               
               
                 AAATACTAATTAAGTTAATATTGTAAGATAGATAATATGGCAAACAAATA 
               
               
                   
               
               
                 AATAATTTTCAGTTGAGAATATGTACAAAATTAAAGTTGATTTTACAAGA 
               
               
                   
               
               
                 AACAAGTAGTACTAATTAAAATGGTAGATTTACATATAGTGTTCGTTAAA 
               
               
                   
               
               
                 TTTGAAAAAAGAAAACGACTAATTCATTGGAAAATTGATTGTGGAAGAAA 
               
               
                   
               
               
                 TCAAAATTTGGTGGCTGAACCGCCCTAGTTACTTAACACAATTCATAAAA 
               
               
                   
               
               
                 CAACCCTACACTACTTACCCTACACTATATACGTATATTTTTTTTTTATA 
               
               
                   
               
               
                 TTCCATTTGTTAATAAAATAAATATGCATTGTTATTATGTGTACCAAGAT 
               
               
                   
               
               
                 GCTAATACGGTGACGTTTTATATAAGTTTATAATTTTTTATTAAAGTAAA 
               
               
                   
               
               
                 ACAATTGTCCCTCAATATTAAATTAAAGAAAATAAGTAGGTTTTAGCATT 
               
               
                   
               
               
                 TCTCATGATAAATAAGTAGGAGTAGTAAGTATATTAATTTATATGCATAT 
               
               
                   
               
               
                 GTTAAAAGGCAACACAGTGATAGGTTGTGTAGTACGTACCCAAACCAAAT 
               
               
                   
               
               
                 TCCAGCCTAATCCATTATTAATGAGCAAGTCCTTGATAAAATAAGGTGCA 
               
               
                   
               
               
                 TGTTGTTAGATCAAATAACCCGTCCCGAAATCCAATATATATATATATAT 
               
               
                   
               
               
                 AATATTCAAACTCTCTCTTTCTATCTTCGTACAATTTAATAGAAGTAACC 
               
               
                   
               
               
                 ATGGG 
               
               
                   
               
               
                 (800 bp) 
               
               
                 Hexanoyl-CoA synthetase (AAE1-1′) promoter 
               
               
                 SEQ ID NO: 12 
               
               
                 ATAATGTTATTTATTATTTAATACAATATGACATTTATACAGCGTACCAA 
               
               
                   
               
               
                 ACGAGCCCATAATGTATTGCAATTGTATTGTTGTACGAATTAAAATAATA 
               
               
                   
               
               
                 AAATACTAATTAAGTTAATATTGTAAGATAGATAATATGGCAAACAAATA 
               
               
                   
               
               
                 AATAATTTTCAGTTGAGAATATGTACAAAATTAAAGTTGATTTTACAAGA 
               
               
                   
               
               
                 AACAAGTAGTACTAATTAAAATGGTAGATTTACATATAGTGTTCGTTAAA 
               
               
                   
               
               
                 TTTGAAAAAAGAAAACGACTAATTCATTGGAAAATTGATTGTGGAAGAAA 
               
               
                   
               
               
                 TCAAAATTTGGTGGCTGAACCGCCCTAGTTACTTAACACAATTCATAAAA 
               
               
                   
               
               
                 CAACCCTACACTACTTACCCTACACTATATACGTATATTTTTTTTTTATA 
               
               
                   
               
               
                 TTCCATTTGTTAATAAAATAAATATGCATTGTTATTATGTGTACCAAGAT 
               
               
                   
               
               
                 GCTAATACGGTGACGTTTTATATAAGTTTATAATTTTTTATTAAAGTAAA 
               
               
                   
               
               
                 ACAATTGTCCCTCAATATTAAATTAAAGAAAATAAGTAGGTTTTAGCATT 
               
               
                   
               
               
                 TCTCATGATAAATAAGTAGGAGTAGTAAGTATATTAATTTATATGCATAT 
               
               
                   
               
               
                 GTTAAAAGGCAACACAGTGATAGGTTGTGTAGTACGTACCCAAACCAAAT 
               
               
                   
               
               
                 TCCAGCCTAATCCATTATTAATGAGCAAGTCCTTGATAAAATAAGGTGCA 
               
               
                   
               
               
                 TGTTGTTAGATCAAATAACCCGTCCCGAAATCCAATATATATATATATAT 
               
               
                   
               
               
                 AATATTCAAACTCTCTCTTTCTATCTTCGTACAATTTAATAGAAGTAATA 
               
               
                   
               
               
                 (1000 bp) 
               
               
                 Hexanoyl-CoA synthetase (AAE3) promoter 
               
               
                 SEQ ID NO: 13 
               
               
                 TAAAAATTAGATTATATTTTTATCAGAATTTTTTATTTATATTTTTCATC 
               
               
                   
               
               
                 GAGTTACGTTGATTTAACGTATGTTGACGGTGCTTATAATTGTCTATTAT 
               
               
                   
               
               
                 TTTTAAGTTATATACACGCACTACTTACGTAAATTTAGTTGGCAGATTTT 
               
               
                   
               
               
                 CTGTCTCTATGGGCTATATTGTTGGACATCAGCTATCTCATTAATTAATA 
               
               
                   
               
               
                 ATACCATACTTTTTCTTAAAAGTTAAATAAAACAAAATTATACACGCACT 
               
               
                   
               
               
                 GATTCGAATGGTGTAGCCCACGCACTTGCTAAGTCTGTTACTAGTTCTGT 
               
               
                   
               
               
                 AGGAGTTAATGTGTGGACTGATGCTTGTCCTAGATTTTATCAGCTATCTC 
               
               
                   
               
               
                 ATTAATTAATAATACCATACTTTTTCTTAAAAGTTAAATAAAACAAAATT 
               
               
                   
               
               
                 ATACACGCACTACGATAAGGATTATATAAATGATTAACTAAAAAATATCT 
               
               
                   
               
               
                 ATTTAACATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGG 
               
               
                   
               
               
                 GATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGT 
               
               
                   
               
               
                 AGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAA 
               
               
                   
               
               
                 GGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCA 
               
               
                   
               
               
                 CGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCA 
               
               
                   
               
               
                 TCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGT 
               
               
                   
               
               
                 GATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGC 
               
               
                   
               
               
                 GAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCC 
               
               
                   
               
               
                 CTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGAAACA 
               
               
                   
               
               
                 CCACCACCACCACCACCACCACGGTGGTCTGGTTCCGCGTGGTTCCCATG 
               
               
                   
               
               
                 ATCAAACAAGTTTGTACAAAAAAGCAGGCTCCGAATTCGCCCTTATGGAG 
               
               
                   
               
               
                 (869 bp) 
               
               
                 Hexanoyl-CoA synthetase (AAE12) promoter 
               
               
                 SEQ ID NO: 14 
               
               
                 GTGACAAACTGAAGAATTCTGTTTATTTTATTTTATACATT 
               
               
                   
               
               
                 AGTGATGTTTTTTACTTTTTTAGAGTTATTTTTATGCCTATGTAATTTGC 
               
               
                   
               
               
                 ATATTGTAGTGGAGTTTATGCAGTTATTTTTTTGTTGTTATTTAGTTGTT 
               
               
                   
               
               
                 TAACTAAGAGAAATAAATATATATATTTTTTATTTGTGCGTTGTTTTTAT 
               
               
                   
               
               
                 TTTATTTTTTAAGTTGACGTAAGTGTTGATGCTCAATTAATTTTTATATT 
               
               
                   
               
               
                 AACATAAAATTGTTATTGTGTTGCTTATTCGTTGCTATTGAGTTATTTCA 
               
               
                   
               
               
                 ATATTGTACTTTTTGAAGTTTTTGTCTTATAAATTTATTTTTATTTGCAT 
               
               
                   
               
               
                 AACATATGATAAACTTTAATGGATTTATATGTTACGGTTAAGTTGTTTTC 
               
               
                   
               
               
                 TAGTTGCTTTTGTGTTGTTGTTTAGTTGTTTTGCGCTGAGAAAAGAAGCC 
               
               
                   
               
               
                 CTATACGTTAAAATTCACTTTGTCCGAATTCGCCACGTGGCAACTACTGC 
               
               
                   
               
               
                 TACGTTCTGACGCTACAGATTGTGAGCGTCAGGCAAGTCTGAATGCTTTG 
               
               
                   
               
               
                 TCCCGCACTCACGTGTGAGTCTCACTCGCCCACATAGCCATATGTGTGCC 
               
               
                   
               
               
                 ACACATCTCCTTGCGCATCTAGGGGAGTTTTTCTACTGTATTTTTGTAAA 
               
               
                   
               
               
                 TAAGAAACTTTGAAAATAGTATATTTGAAATTAATAAATAATATAAATGT 
               
               
                   
               
               
                 TTATTTTTACTTATAGTAGTATAAATATTTATTTAAGATAATTTTAACTT 
               
               
                   
               
               
                 AAAATAGAAAAAATCTCAAAAAAAAATAAAATAAAATAAACTATACCTTT 
               
               
                   
               
               
                 AATTTTTATATTTATGAAAATTTCCCGTATAAAAGGGAAGATTTTTTTTT 
               
               
                   
               
               
                 TTAAAATTAGTAGGTAAGTAATAGGATG 
               
               
                   
               
               
                 (2351 bp) 
               
               
                 Hexanoyl-CoA synthetase (AAE1-1) ORF 
               
               
                 SEQ ID NO: 15 
               
            
           
           
               
               
            
               
                 1 
                 tcttggagtg tatatataga tatatatata catttctcca tttttggcca cccaaattac 
               
               
                   
               
               
                 61 
                 ctaaattgaa ttcgtcgtgt tcgtctttct ccgccataaa taacaattga tttccttcgt 
               
               
                   
               
               
                 121 
                 tctttcaagt ttbbgtcaac tgagtactga gatccagtct gagtcgacac ttgtagaggt 
               
               
                   
               
               
                 181 
                 tcgaaaatgg cgcagaagaa gtttattttt gaggttgaaa aggctaagga agctaaggat 
               
               
                   
               
               
                 241 
                 ggaaggcctt ccatgggacc tgtttatcga agtatgtacg ccaaggatgg ctttcctcct 
               
               
                   
               
               
                 301 
                 ccgattgatg gattggattg ttgttgggat gtgtttaggc tttctgtgga gagaaatcct 
               
               
                   
               
               
                 361 
                 ggaaacaaaa tgctcggtcg ccgggaggtt gtggatggga aggctggaaa gtatgtttgg 
               
               
                   
               
               
                 421 
                 caaacttata aggaggttta tgattgtgtg ataaaacttg gaaatgccat gcgcagttgt 
               
               
                   
               
               
                 481 
                 ggtttcggag aaggagacaa atgtggtatc tatggtgcca attgctctga atggattatg 
               
               
                   
               
               
                 541 
                 agcatgcagg cttgcaatgc tcatggactc tattgtgttc cgttgtatga caccttaggt 
               
               
                   
               
               
                 601 
                 gctaatgcaa ttgagtttgt cataaaacat gccgaggtta caattgcttt tgcagaagag 
               
               
                   
               
               
                 661 
                 aaaaagcttc ctgagctgtt gaaaacactt cctaacacca cagactattt aaaaacactt 
               
               
                   
               
               
                 721 
                 gtgagctttg ggaaggtcac accggaacaa agggaatctg ttgaaaagtt tggattgaag 
               
               
                   
               
               
                 781 
                 atctattcct gggaagagtt cttgcaattg ggtgattgta aagatttcga tcttccagtg 
               
               
                   
               
               
                 841 
                 aaaaagaaaa ctgatatatg cactataatg tatactagtg gaactactgg tgacccaaag 
               
               
                   
               
               
                 901 
                 ggagtactga tatccaatga gagcatcatt tctcttttgg ctggggtgag gcgattgctt 
               
               
                   
               
               
                 961 
                 gagagtgtaa atgaaaagtt gaccgagaaa gatgtatttc tttcatatct tccccttgca 
               
               
                   
               
               
                 1021 
                 catatcttcg atcgggtgat tgaggagtta tttattttgc atggtgcctc aatcgggttc 
               
               
                   
               
               
                 1081 
                 tggcgtggcg atgtcaaatt attacttgaa gacattgggg agctaaaacc aactattttc 
               
               
                   
               
               
                 1141 
                 tgtgccgttc cccgtgtgct ggatagagtc tatgctggtt tgacgcagaa gatttctgct 
               
               
                   
               
               
                 1201 
                 gggggattct taaagaagac tctcttcaat ttcgcatact cacgcaagta taataacatg 
               
               
                   
               
               
                 1261 
                 cgaaaggggc ttaaacacgg ggacgcagct ccattatgtg acaaaattgt atttagtaag 
               
               
                   
               
               
                 1321 
                 gtgaagcaag gtttgggagg taatgtgcgg cttattctgt ctggagctgc gcctctagct 
               
               
                   
               
               
                 1381 
                 cctcatgttg aagcttactt acgagttgtg acatgtgctc atgtttgcca aggatatggg 
               
               
                   
               
               
                 1441 
                 ctaactgaaa cttgtgcggg gacatttgtc tcgataccaa atgaactacc aatgctcggt 
               
               
                   
               
               
                 1501 
                 acagttggtc ctccagtacc caatgttgat gtatgtctgg aatcagttcc ggaaatggga 
               
               
                   
               
               
                 1561 
                 tatgatgccc ttgcagaagt accgcgtgga gaagtatgtg tgaagggaag caccttattc 
               
               
                   
               
               
                 1621 
                 tctgggtact acaaacgtga agacttaacc aaggaggtca tgattgatgg gtggttccat 
               
               
                   
               
               
                 1681 
                 acaggtgatg ttggtgagtg gcaagcagat ggaagcttga aaatcattga ccgtaagaaa 
               
               
                   
               
               
                 1741 
                 aatattttca aactttctca aggagaatat gtggcagttg agaacttaga gaacatttat 
               
               
                   
               
               
                 1801 
                 ggtcttgttt ctgacattga ctcaatatgg gtttacggga acagcttcga gtccttcctc 
               
               
                   
               
               
                 1861 
                 gttgctgtga ttaacccgaa ccagcaagca cttgaacgtt gggccggaga gaataatata 
               
               
                   
               
               
                 1921 
                 tctggtgact tcaagtccat ttgtgaaaat ccaagagcaa aggaatacat tttgggagag 
               
               
                   
               
               
                 1981 
                 ctcaataaga ttggcaaaga gaaaaagttg aaaggttttg agttcataag agctgttcac 
               
               
                   
               
               
                 2041 
                 cttgacccag aggcatttga catagaacgt gaccttatta ctccaacata taagaaaaag 
               
               
                   
               
               
                 2101 
                 agaccccagt tgctcaaata ctaccagagt gttattgata acatgtacaa ggaaggaaac 
               
               
                   
               
               
                 2161 
                 aagcccagga cctgaaagat ataatagaca tttagagctt cactttctaa tatttcttca 
               
               
                   
               
               
                 2221 
                 cacacttccc attttcatct ctctcttact tattacacta taaataatta acaaggttta 
               
               
                   
               
               
                 2281 
                 ctgtgttgta aatgacattt taatattttc atagtctcaa tgtttcaatt cttagaggac 
               
               
                   
               
               
                 2341 
                 gttttgattg g 
               
               
                   
               
            
           
           
               
            
               
                 (420 bp) 
               
               
                 CBDA Synthase (CBDAS) promoter 
               
               
                 SEQ ID NO: 16 
               
               
                 GTTAATAAACATACTTGTGAGTAGATCTAAGATCCTGGTAAAATAATTCCCAACATATT 
               
               
                   
               
               
                 TCAATCGTAAATTCATGAGTGATTCTTATCACTTCTTAAAAAAAAGATAT 
               
               
                   
               
               
                 AAAGATCCATAGATAAATAT TTGCTAGTCC TTTCTTTCCA TAGTCAACTT 
               
               
                   
               
               
                 CAATATGGCA TCTTAACTTC TTTCCATCAA CAAATGATGC TCAACTACTT 
               
               
                   
               
               
                 AATGTACAAT TTATATTTAT TTTTAGTAAG GGATACACCT AACAATGATG 
               
               
                   
               
               
                 CCTAATTTTG GTGAATTTTT TTTTACCACA TGTGACTTAA TGATATCAAA 
               
               
                   
               
               
                 TTTGGAAATA TTTAGTTAAT TTATTTGCCC CTGCTCCATT ATATAAAGCT 
               
               
                   
               
               
                 ATAAATAGGA TAGTTCTTAA TTCATAGTAA TTCAAAAATT ATTAGAACTA 
               
               
                   
               
               
                 AAGAACCATG G 
               
               
                   
               
               
                 (416 bp) 
               
               
                 CBDA Synthase 1 (CBDAS1) promoter 
               
               
                 SEQ ID NO: 17 
               
               
                 GTTAATAAACATACTTGTGAGTAGATCTAAGATCCTGGTAAAATAATTCCCAACATATT 
               
               
                   
               
               
                 TCAATCGTAAATTCATGAGTGATTCTTATCACTTCTTAAAAAAAAGATAT 
               
               
                   
               
               
                 AAAGATCCATAGATAAATAT TTGCTAGTCC TTTCTTTCCA TAGTCAACTT 
               
               
                   
               
               
                 CAATATGGCA TCTTAACTTC TTTCCATCAA CAAATGATGC TCAACTACTT 
               
               
                   
               
               
                 AATGTACAAT TTATATTTAT TTTTAGTAAG GGATACACCT AACAATGATG 
               
               
                   
               
               
                 CCTAATTTTG GTGAATTTTT TTTTACCACA TGTGACTTAA TGATATCAAA 
               
               
                   
               
               
                 TTTGGAAATA TTTAGTTAAT TTATTTGCCC CTGCTCCATT ATATAAAGCT 
               
               
                   
               
               
                 ATAAATAGGA TAGTTCTTAA TTCATAGTAA TTCAAAAATT ATTAGAACTA 
               
               
                   
               
               
                 AAGAAAA 
               
               
                   
               
               
                 (535 bp) 
               
               
                 CBDA Synthase (CBDAS) 20800 promoter 
               
               
                 SEQ ID NO: 18 
               
               
                 AGACGTT ATAGCTTTATTGTCTAAATT TCTTGGGGTA GTTTTGTCCC ATGTTGCTCG 
               
               
                   
               
               
                 CTTACTTTAGAAGTTCAAAG TTTGACAAAA CATGCTATTC GGTTATACAA 
               
               
                   
               
               
                 TGAGCTATCC 
               
               
                   
               
               
                 TAGTTCAAGG AGATTCCTGT GCTATTTGTG GATGTCTACA TTGTAAATTC 
               
               
                   
               
               
                 ATGAGTGATT CTTATAACTT TATTAAAAAA AAGACAAATA GATCCATAGA 
               
               
                   
               
               
                 TAAATATTTG CAAGTGCTTT CTTGTCATAG CAATTTTTTT TTACATAGGT 
               
               
                   
               
               
                 AATTTAAAAA ATTCATCTTA ACTTTTTTTC ATCAATAAAT GATGCTCAAT 
               
               
                   
               
               
                 TATTCAATGC ACCATGTACA TTTATTTTTA ATAAGGGCTG CACCTAACAA 
               
               
                   
               
               
                 AGGTGCCTAA TTTTGGTAAA AAAGAAATTA CGGCATGTGA ATATTTAATG 
               
               
                   
               
               
                 AGCATCAAAT TACAAAATAT TTAGCTAATT TCTTTACCCC CACTCCAATC 
               
               
                   
               
               
                 TATAAAGCTA TAAATAGGAT AATTTTCCAT TCATAGTAAT TTCCAACATT 
               
               
                   
               
               
                 ACGACTAAAG AACCATGG 
               
               
                   
               
               
                 (531 bp) 
               
               
                 CBDA Synthase (CBDAS) 20800′ promoter 
               
               
                 SEQ ID NO: 19 
               
               
                 AGACGTT ATAGCTTTATTGTCTAAATT TCTTGGGGTA GTTTTGTCCC ATGTTGCTCG 
               
               
                   
               
               
                 CTTACTTTAGAAGTTCAAAG TTTGACAAAA CATGCTATTC GGTTATACAA 
               
               
                   
               
               
                 TGAGCTATCC 
               
               
                   
               
               
                 TAGTTCAAGG AGATTCCTGT GCTATTTGTG GATGTCTACA TTGTAAATTC 
               
               
                   
               
               
                 ATGAGTGATT CTTATAACTT TATTAAAAAA AAGACAAATA GATCCATAGA 
               
               
                   
               
               
                 TAAATATTTG CAAGTGCTTT CTTGTCATAG CAATTTTTTT TTACATAGGT 
               
               
                   
               
               
                 AATTTAAAAA ATTCATCTTA ACTTTTTTTC ATCAATAAAT GATGCTCAAT 
               
               
                   
               
               
                 TATTCAATGC ACCATGTACA TTTATTTTTA ATAAGGGCTG CACCTAACAA 
               
               
                   
               
               
                 AGGTGCCTAA TTTTGGTAAA AAAGAAATTA CGGCATGTGA ATATTTAATG 
               
               
                   
               
               
                 AGCATCAAAT TACAAAATAT TTAGCTAATT TCTTTACCCC CACTCCAATC 
               
               
                   
               
               
                 TATAAAGCTA TAAATAGGAT AATTTTCCAT TCATAGTAAT TTCCAACATT 
               
               
                   
               
               
                 ACGACTAAAG AAAA 
               
               
                   
               
               
                 (1635 bp) 
               
               
                 CBDA Synthase (CBDAS) ORF 
               
               
                 SEQ ID NO: 20 
               
            
           
           
               
               
            
               
                 1 
                 atgaagtgct caacattctc cttttggttt gtttgcaaga taatattttt ctttttctca 
               
               
                   
               
               
                 61 
                 ttcaatatcc aaacttccat tgctaatcct cgagaaaact tccttaaatg cttctcgcaa 
               
               
                   
               
               
                 121 
                 tatattccca ataatgcaac aaatctaaaa ctcgtataca ctcaaaacaa cccattgtat 
               
               
                   
               
               
                 181 
                 atgtctgtcc taaattcgac aatacacaat cttagattca cctctgacac aaccccaaaa 
               
               
                   
               
               
                 241 
                 ccacttgtta tcgtcactcc ttcacatgtc tctcatatcc aaggcactat tctatgctcc 
               
               
                   
               
               
                 301 
                 aagaaagttg gcttgcagat tcgaactcga agtggtggtc atgattctga gggcatgtcc 
               
               
                   
               
               
                 361 
                 tacatatctc aagtcccatt tgttatagta gacttgagaa acatgcgttc aatcaaaata 
               
               
                   
               
               
                 421 
                 gatgttcata gccaaactgc atgggttgaa gccggagcta cccttggaga agtttattat 
               
               
                   
               
               
                 481 
                 tgggttaatg agaaaaatga gaatcttagt ttggcggctg ggtattgccc tactgtttgc 
               
               
                   
               
               
                 541 
                 gcaggtggac actttggtgg aggaggctat ggaccattga tgagaaacta tggcctcgcg 
               
               
                   
               
               
                 601 
                 gctgataata tcattgatgc acacttagtc aacgttcatg gaaaagtgct agatcgaaaa 
               
               
                   
               
               
                 661 
                 tctatggggg aagatctctt ttgggcttta cgtggtggtg gagcagaaag cttcggaatc 
               
               
                   
               
               
                 721 
                 attgtagcat ggaaaattag actggttgct gtoccaaagt ctactatgtt tagtgttaaa 
               
               
                   
               
               
                 781 
                 aagatcatgg agatacatga gcttgtcaag ttagttaaca aatggcaaaa tattgcttac 
               
               
                   
               
               
                 841 
                 aagtatgaca aagatttatt actcatgact cacttcataa ctaggaacat tacagataat 
               
               
                   
               
               
                 901 
                 caagggaaga ataagacagc aatacacact tacttctctt cagttttcct tggtggagtg 
               
               
                   
               
               
                 961 
                 gatagtctag tcgacttgat gaacaagagt tttcctgagt tgggtattaa aaaaacggat 
               
               
                   
               
               
                 1021 
                 tgcagacaat tgagctggat tgatactatc atottctata gtggtgttgt aaattacgac 
               
               
                   
               
               
                 1081 
                 actgataatt ttaacaagga aattttgctt gatagatccg ctgggcagaa cggtgctttc 
               
               
                   
               
               
                 1141 
                 aagattaagt tagactacgt taagaaacca attccagaat ctgtatttgt ccaaattttg 
               
               
                   
               
               
                 1201 
                 gaaaaattat atgaagaaga tataggagct gggatgtatg cgttgtaccc ttacggtggt 
               
               
                   
               
               
                 1261 
                 ataatggatg agatttcaga atcagcaatt ccattocctc atcgagctgg aatcttgtat 
               
               
                   
               
               
                 1321 
                 gagttatggt acatatgtag ttgggagaag caagaagata acgaaaagca tctaaactgg 
               
               
                   
               
               
                 1381 
                 attagaaata tttataactt catgactect tatgtgtcca aaaatccaag attggcatat 
               
               
                   
               
               
                 1441 
                 ctcaattata gagaccttga tataggaata aatgatccca agaatccaaa taattacaca 
               
               
                   
               
               
                 1501 
                 caagcacgta tttggggtga gaagtatttt ggtaaaaatt ttgacaggct agtaaaagtg 
               
               
                   
               
               
                 1561 
                 aaaaccctgg ttgatcccaa taactttttt agaaacgaac aaagcatccc acctcttcca 
               
               
                   
               
               
                 1621 
                 cggcatcgtc attaa 
               
               
                   
               
            
           
           
               
            
               
                 (800 bp) 
               
               
                 THCA Synthase (THCAS) 19603 promoter 
               
               
                 SEQ ID NO: 21 
               
               
                 CTTATCACTC ATTCGGGAGC AACTTAAAAA AATTACAATT ATTATGAAAG 
               
               
                   
               
               
                 ACGGCACAAA AAGCGAATTA ATTACCTAAT CGTAAATAAA AGAAATTTTA 
               
               
                   
               
               
                 CATAAATATA TTATATAATT TTATATTATT CATAAAAAAT ATAAAATAGT 
               
               
                   
               
               
                 ATTAGACTGA ATTGAGATTT GATTCGTAAC TAAAAATTAA TTTCTTTAAT 
               
               
                   
               
               
                 GTAAAATTTT AAAAACAAAT TATTAAAACC GCGTGAAGCG CGGATCTATT 
               
               
                   
               
               
                 CCCTAGTTCA ATAATAAAGT ACATACTAGA GGAGGTTAGA ATTTATAATT 
               
               
                   
               
               
                 TTTCATTTAA ATATTTGAAT TTACTTTACT GTTCTTTGTT ATTCTTCATT 
               
               
                   
               
               
                 TAATTTTGCT ATTTGTTATT TTACTTTTCA AAAATTATAA TTTTAATTAC 
               
               
                   
               
               
                 CAAATAAAAA GTAAAATAGA TATTGCTACT TGATATTCAC TCTTTATGGG 
               
               
                   
               
               
                 AACCATAATA AACTATAAAA GTCATTATGT GTACTTGCTA CCATAGGCAC 
               
               
                   
               
               
                 CTATATCCCA CAAACTAGCT ACCATAGCCA ATTTCTTGTT TTTTGTTTCC 
               
               
                   
               
               
                 AATATCCAAT TTTTATTGAT GCCAAACTAT TCAATGTACA ATCTACATTT 
               
               
                   
               
               
                 ATTTTCAATA AGGGCTTCAC CTAACAAAGG TGCCTAATTT TAGTTGATTT 
               
               
                   
               
               
                 ATTTTTTATC ACATGTGACT ATTTAATGAC TATCAAATTA TAAAATATTT 
               
               
                   
               
               
                 AAGTCAATTT ATTTGCCCCA ACTCCAATAT ATAATATTAT AAATAGGATA 
               
               
                   
               
               
                 GTTCTCAATT CCTAATAATT CAAAAAATCA TTAGGACTGA AGAACCATGG 
               
               
                   
               
               
                 (796 bp) 
               
               
                 THCA Synthase (THCAS) 19603′ promoter 
               
               
                 SEQ ID NO: 22 
               
               
                 CTTATCACTC ATTCGGGAGC AACTTAAAAA AATTACAATT ATTATGAAAG 
               
               
                   
               
               
                 ACGGCACAAA AAGCGAATTA ATTACCTAAT CGTAAATAAA AGAAATTTTA 
               
               
                   
               
               
                 CATAAATATA TTATATAATT TTATATTATT CATAAAAAAT ATAAAATAGT 
               
               
                   
               
               
                 ATTAGACTGA ATTGAGATTT GATTCGTAAC TAAAAATTAA TTTCTTTAAT 
               
               
                   
               
               
                 GTAAAATTTT AAAAACAAAT TATTAAAACC GCGTGAAGCG CGGATCTATT 
               
               
                   
               
               
                 CCCTAGTTGA ATAATAAAGT AGATAGTAGA GGAGGTTAGA ATTTATAATT 
               
               
                   
               
               
                 TTTCATTTAA ATATTTGAAT TTACTTTACT GTTCTTTGTT ATTCTTCATT 
               
               
                   
               
               
                 TAATTTTGCT ATTTGTTATT TTACTTTTCA AAAATTATAA TTTTAATTAC 
               
               
                   
               
               
                 CAAATAAAAA GTAAAATAGA TATTGGTACT TGATATTCAC TCTTTATGGG 
               
               
                   
               
               
                 AACCATAATA AACTATAAAA GTCATTATGT GTACTTGCTA CCATAGGCAC 
               
               
                   
               
               
                 CTATATCCCA CAAACTAGCT ACCATAGCCA ATTTCTTGTT TTTTGTTTCC 
               
               
                   
               
               
                 AATATCCAAT TTTTATTGAT GCCAAACTAT TCAATGTACA ATGTACATTT 
               
               
                   
               
               
                 ATTTTCAATA AGGGCTTCAC CTAACAAAGG TGCCTAATTT TAGTTGATTT 
               
               
                   
               
               
                 ATTTTTTATC ACATGTGACT ATTTAATGAC TATCAAATTA TAAAATATTT 
               
               
                   
               
               
                 AAGTCAATTT ATTTGCCCCA ACTCCAATAT ATAATATTAT AAATAGGATA 
               
               
                   
               
               
                 GTTCTCAATT CCTAATAATT CAAAAAATCA TTAGGACTGA AGAAAA 
               
               
                   
               
               
                 (796 bp) 
               
               
                 THCA Synthase (THCAS) 50320 promoter 
               
               
                 SEQ ID NO: 23 
               
               
                 TTATTTAAAT TTCTCAGAGA GATATAGAGA ATTTACAATA GGTCAAGTTG 
               
               
                   
               
               
                 TATATTTTTT AATCCGAATA ATTAATGCTT TTGAATATCC ATCATCAATA 
               
               
                   
               
               
                 TTGAATAATA AAGTAGATAG CAGAGGAGAT TAGAACTCAT AATTTTTCAT 
               
               
                   
               
               
                 TTATATTTTT GAATTTAGTT TACTGTTCTT TATTATTCTT CATTTAATTT 
               
               
                   
               
               
                 TGCTATATGT TATTTTACTT TTCAAAAATT CTAATTTTGA TTACCAAATA 
               
               
                   
               
               
                 AAAAATAAAA TATATATTGG TACTTCATAT TTAGTTTTTA TGGGAATGAT 
               
               
                   
               
               
                 AATGAATGAT ATTGTAATAA ACTATAAAAG TAATTTTGTA TACTTGCATA 
               
               
                   
               
               
                 GTTCTTAATT TTTACCCAAA AAAATATATA TTAATGAAAA AAAAAGGTGG 
               
               
                   
               
               
                 AAAGTGCCAT AGGCACCTAT ATCCCACAAA CTAGCTATAA GATATTGAAA 
               
               
                   
               
               
                 GACAAATAGA TCCATAGATA AATATTTGCG TGTCCTTTCT TTTCATATCT 
               
               
                   
               
               
                 AATTTTTTGT TTTTTTATTT TTTTCCAATA GTCAATTTTG GCAGCTTAAC 
               
               
                   
               
               
                 TTCTTTCCAT AAATAATGAT GCCAAACTAT TCAATGTAAA ATTTAGATTT 
               
               
                   
               
               
                 ATTTTCATTA AGGGCTTCAC CTAACAAATG TGCCTAATTT TTGTGGATTT 
               
               
                   
               
               
                 TTTTACCATA TGTCGCTATT TAATGACTAC CAAATTATAG AATTATTTAA 
               
               
                   
               
               
                 GTCAATTTCT CAGTCCCCGC TCCAATATAT AAAGTTATAG AAAGGACAAT 
               
               
                   
               
               
                 TCTTAATTCA TAGTAATTCA AAAATCATTA AGACTAAAAA CCATGG 
               
               
                   
               
               
                 (792 bp) 
               
               
                 THCA Synthase (THCAS) 50320′ promoter 
               
               
                 SEQ ID NO: 24 
               
               
                 TTATTTAAAT TTCTCAGAGA GATATAGAGA ATTTACAATA GGTCAAGTTG 
               
               
                   
               
               
                 TATATTTTTT AATCCGAATA ATTAATGCTT TTGAATATCC ATCATCAATA 
               
               
                   
               
               
                 TTGAATAATA AAGTAGATAG CAGAGGAGAT TAGAACTCAT AATTTTTCAT 
               
               
                   
               
               
                 TTATATTTTT GAATTTAGTT TACTGTTCTT TATTATTCTT CATTTAATTT 
               
               
                   
               
               
                 TGCTATATGT TATTTTACTT TTCAAAAATT CTAATTTTGA TTACCAAATA 
               
               
                   
               
               
                 AAAAATAAAA TATATATTGG TACTTCATAT TTAGTTTTTA TGGGAATGAT 
               
               
                   
               
               
                 AATGAATGAT ATTGTAATAA ACTATAAAAG TAATTTTGTA TACTTGCATA 
               
               
                   
               
               
                 GTTCTTAATT TTTACCCAAA AAAATATATA TTAATGAAAA AAAAAGGTGG 
               
               
                   
               
               
                 AAAGTGCCAT AGGCACCTAT ATCCCACAAA CTAGCTATAA GATATTGAAA 
               
               
                   
               
               
                 GACAAATAGA TCCATAGATA AATATTTGCG TGTCCTTTCT TTTCATATCT 
               
               
                   
               
               
                 AATTTTTTGT TTTTTTATTT TTTTCCAATA GTCAATTTTG GCAGCTTAAC 
               
               
                   
               
               
                 TTCTTTCCAT AAATAATGAT GCCAAACTAT TCAATGTAAA ATTTAGATTT 
               
               
                   
               
               
                 ATTTTCATTA AGGGCTTCAC CTAACAAATG TGCCTAATTT TTGTGGATTT 
               
               
                   
               
               
                 TTTTACCATA TGTCGCTATT TAATGACTAC CAAATTATAG AATTATTTAA 
               
               
                   
               
               
                 GTCAATTTCT CAGTCCCCGC TCCAATATAT AAAGTTATAG AAAGGACAAT 
               
               
                   
               
               
                 TCTTAATTCA TAGTAATTCA AAAATCATTA AGACTAAAAA AA 
               
               
                   
               
               
                 (720 bp) 
               
               
                 THCA Synthase (THCAS) 1330 promoter 
               
               
                 SEQ ID NO: 25 
               
               
                 TTTTCAATTG ATTTAATTTC TTATATTGAT ATAAAGAATT TGCAATACAT 
               
               
                   
               
               
                 TGAGTTTCTT AACCCGAATA TTAAACGGTT TTGAATATCT TCATCATTGA 
               
               
                   
               
               
                 TTGAATAATA AAGTGGATAG TAGAGGGAAT TAGAATCCAT AATTTTTTAT 
               
               
                   
               
               
                 TTATATATTT GAAGTTAGTT TATTATTCTT TGCTATCCTT CATTTAATTT 
               
               
                   
               
               
                 TGCTATTTGT TATTTTAGTT TCAAAAATTT ATTTTTCATT ACAAAATAAA 
               
               
                   
               
               
                 AAATAAGATA GGATATTGGT ACTTGATAAG TCTTCTTTGT GGAAACGATA 
               
               
                   
               
               
                 ATCGGTATTA TTAGGTATAT TTGCATATTT CAATATTATT GCAAAAAATA 
               
               
                   
               
               
                 AATATATTAA TAAATAAAGT GGAAGGTGCC ATAGGTACCT ATATCCACAA 
               
               
                   
               
               
                 ACTAGCATAT TGAAAGAALA TGGATCCAAG GATAAATATT TGCAAGTCTT 
               
               
                   
               
               
                 TTTTTTTTTG CATAGCCAAT TTCTTTTTTT ATTATTATTT TAATAGAATA 
               
               
                   
               
               
                 TTTCAAAAGG GCATCTAACA TTTATTTTTA ATAAGGACTG CACCTAACAA 
               
               
                   
               
               
                 AGGTGCCTAA TTTTTGTGAA CTTTTTTTTA CCACATGTGA CTATTTAATG 
               
               
                   
               
               
                 AGTACTAAAT TATGAAATAT TTAGTTAATT TCTTTGCCCC CGCTCCAATA 
               
               
                   
               
               
                 TATAATGCTA TAAATAGCAT AATTTTCTAT TCATAGTAAT TCAAAAATCA 
               
               
                   
               
               
                 TTAGGACTAA AGAACCATGG 
               
               
                   
               
               
                 (716 bp) 
               
               
                 THCA Synthase (THCAS) 1330′ promoter 
               
               
                 SEQ ID NO: 26 
               
               
                 TTTTCAATTG ATTTAATTTC TTATATTGAT ATAAAGAATT TGCAATACAT 
               
               
                   
               
               
                 TGAGTTTCTT AACCCGAATA TTAAACGGTT TTGAATATCT TCATCATTGA 
               
               
                   
               
               
                 TTGAATAATA AAGTGGATAG TAGAGGGAAT TAGAATCCAT AATTTTTTAT 
               
               
                   
               
               
                 TTATATATTT GAAGTTAGTT TATTATTCTT TGCTATCCTT CATTTAATTT 
               
               
                   
               
               
                 TGCTATTTGT TATTTTAGTT TCAAAAATTT ATTTTTCATT ACAAAATAAA 
               
               
                   
               
               
                 AAATAAGATA GGATATTGGT ACTTGATAAG TCTTCTTTGT GGAAACGATA 
               
               
                   
               
               
                 ATCGGTATTA TTAGGTATAT TTGCATATTT CAATATTATT GCAAAAAATA 
               
               
                   
               
               
                 AATATATTAA TAAATAAAGT GGAAGGTGCC ATAGGTACCT ATATCCACAA 
               
               
                   
               
               
                 ACTAGCATAT TGAAAGAAAA TGGATCCAAG GATAAATATT TGCAAGTCTT 
               
               
                   
               
               
                 TTTTTTTTTG CATAGCCAAT TTCTTTTTTT ATTATTATTT TAATAGAATA 
               
               
                   
               
               
                 TTTCALLAGG GCATCTAACA TTTATTTTTA ATAAGGACTG CACCTAACAA 
               
               
                   
               
               
                 AGGTGCCTAA TTTTTGTGAA CTTTTTTTTA CCACATGTGA CTATTTAATG 
               
               
                   
               
               
                 AGTACTAAAT TATGAAATAT TTAGTTAATT TCTTTGCCCC CGCTCCAATA 
               
               
                   
               
               
                 TATAATGCTA TAAATAGCAT AATTTTCTAT TCATAGTAAT TCAAAAATCA 
               
               
                   
               
               
                 TTAGGACTAA AGAAAG 
               
               
                   
               
               
                 (1635 bp) 
               
               
                 THCA Synthase (THCAS) ORF 
               
               
                 SEQ ID NO: 27 
               
            
           
           
               
               
            
               
                 1 
                 atgaattgct cagcattttc cttttggttt gtttgcaaaa taatattttt ctttctctca 
               
               
                   
               
               
                 61 
                 ttcaatatcc aaatttcaat agctaatcct caagaaaact tccttaaatg cttctcggaa 
               
               
                   
               
               
                 121 
                 tatattccta acaatccagc aaatccaaaa ttcatataca ctcaacacga ccaattgtat 
               
               
                   
               
               
                 181 
                 atgtctgtcc tgaattcgac aatacaaaat cttagattca cctatgatac aaccccaaaa 
               
               
                   
               
               
                 241 
                 ccactcgtta ttgtcactcc ttcaaatgtc tcccatatcc aggccagtat tctctgctcc 
               
               
                   
               
               
                 301 
                 aagaaagttg gtttgcagat tcgaactcga agcggtggcc atgatgctga gggtatgtcc 
               
               
                   
               
               
                 361 
                 tacatatctc aagtcccatt tgttgtagta gacttgagaa acatgcattc gatcaaaata 
               
               
                   
               
               
                 421 
                 gatgttcata gccaaactgc gtgggttgaa gccggagcta cccttggaga agtttattat 
               
               
                   
               
               
                 481 
                 tggatcaatg agaagaatga gaattttagt tttcctggtg ggtattgccc tactgttggc 
               
               
                   
               
               
                 541 
                 gtaggtggac actttagtgg aggaggctat ggagcattga tgcgaaatta tggccttgcg 
               
               
                   
               
               
                 601 
                 gctgataata tcattgatgc acacttagtc aatgttgatg gaaaagttct agatcgaaaa 
               
               
                   
               
               
                 661 
                 tccatgggag aagatctatt ttgggctata cgtggtggag gaggagaaaa ctttggaatc 
               
               
                   
               
               
                 721 
                 attgcagcat ggaaaatcaa actggttgtt gtcccatcaa agtctactat attcagtgtt 
               
               
                   
               
               
                 781 
                 aaaaagaaca tggagataca tgggcttgtc aagttattta acaaatggca aaatattgct 
               
               
                   
               
               
                 841 
                 tacaagtatg acaaagattt agtactcatg actcacttca taacaaagaa tattacagat 
               
               
                   
               
               
                 901 
                 aatcatggga agaataagac tacagtacat ggttacttct cttcaatttt tcatggtgga 
               
               
                   
               
               
                 961 
                 gtggatagtc tagtcgactt gatgaacaag agctttcctg agttgggtat taaaaaaact 
               
               
                   
               
               
                 1021 
                 gattgcaaag aatttagctg gattgataca accatcttct acagtggtgt tgtaaatttt 
               
               
                   
               
               
                 1081 
                 aacactgcta attttaaaaa ggaaattttg cttgatagat cagctgggaa gaagacggct 
               
               
                   
               
               
                 1141 
                 ttctcaatta agttagacta tgttaagaaa ccaattccag aaactgcaat ggtcaaaatt 
               
               
                   
               
               
                 1201 
                 ttggaaaaat tatatgaaga agatgtagga gctgggatgt atgtgttgta cccttacggt 
               
               
                   
               
               
                 1261 
                 ggtataatgg aggagatttc agaatcagca attccattcc ctcatcgagc tggaataatg 
               
               
                   
               
               
                 1321 
                 tatgaacttt ggtacactgc ttcctgggag aagcaagaag ataatgaaaa gcatataaac 
               
               
                   
               
               
                 1381 
                 tgggttcgaa gtgtttataa ttttacgact ccttatgtgt cccaaaatcc aagattggcg 
               
               
                   
               
               
                 1441 
                 tatctcaatt atagggacct tgatttagga aaaactaatc ctgagagtcc taataattac 
               
               
                   
               
               
                 1501 
                 acacaagcac gtatttgggg tgaaaagtat tttggtaaaa attttaacag gttagttaag 
               
               
                   
               
               
                 1561 
                 gtgaaaacca aagctgatcc caataatttt tttdgaaacg aacaaagtat cccacctctt 
               
               
                   
               
               
                 1621 
                 ccaccgcatc atcat 
               
               
                   
               
            
           
           
               
            
               
                 (804 bp) 
               
               
                 CBCA Synthase (CBCAS) 3498 promoter 
               
               
                 SEQ ID NO: 28 
               
               
                 GATAATTTTT CTTCCAAATT TAATAGTATC TTTTATATAA GTTCAGTCAT 
               
               
                   
               
               
                 GTTTTTCTTA TGTAAGTTAG GCTATTAATT ATTTTGATGT ATTTATTATA 
               
               
                   
               
               
                 TTGTATGTCG TTTACTGCAT TCTGCCAACA TTGGTTTTTG TCGATTTATG 
               
               
                   
               
               
                 ATATATAATA TGATATGCTG TTAAGTTAGA GGTAAGATTC AATTTATTTA 
               
               
                   
               
               
                 AGATTATTTA ATTTGCGCTT TTATTATATA CATCTTCTAG TTGTAATTAA 
               
               
                   
               
               
                 TGGGGTAGAA TTAAGACTGT GTTTTGAATT AATATCGGTA TTAGAATAGG 
               
               
                   
               
               
                 ATTTGCATGA CTATATTTCT ACCTTACCAA GTTTTTTATC TGATAACACC 
               
               
                   
               
               
                 CTTTCACTTA TTATTTTCTT ATTTTTAATT TATTTAT 
               
               
                   
               
               
                 ATTTTTACCATATTCTATTTATACTAATCTTAAGTGGTACATTACCTCCCTGTG 
               
               
                   
               
               
                 GATACGACATATA 
               
               
                   
               
               
                 ATCTGTTTACTATCGTGACCGAAGTATAACTAATTGGGCGACATCACACCTATA 
               
               
                   
               
               
                 TCCCACAAACTAGCTACTATAGTCAATTTCTTGTTTTTTTTCCAATAGCCAATT 
               
               
                   
               
               
                 TTAAATGATGCCAAACTATTCAATGTATAATGTACATTTATTTTAAATAAGGGC 
               
               
                   
               
               
                 TTCACCTAACAAATGTGCCTAATTTTAGTTAATTTATTTTTTTATCGCATCTTA 
               
               
                   
               
               
                 CTATTTAAAGGAACTATCAAATTATAAAATATTTATGTCAATTCATTTGCCCCA 
               
               
                   
               
               
                 ACTCCAATATATAATATTATAAATAGGATAGTTCTCTATTCATAATAATTCAAA 
               
               
                   
               
               
                 ATATCATTAGGACTGAAGAACCATGG 
               
               
                   
               
               
                 (800 bp) 
               
               
                 CBCA Synthase (CBCAS) 3498′ promoter 
               
               
                 SEQ ID NO: 29 
               
               
                 GATAATTTTT CTTCCAAATT TAATAGTATC TTTTATATAA GTTCAGTCAT 
               
               
                   
               
               
                 GTTTTTCTTA TGTAAGTTAG GCTATTAATT ATTTTGATGT ATTTATTATA 
               
               
                   
               
               
                 TTGTATGTCG TTTACTGCAT TCTGCCAACA TTGGTTTTTG TCGATTTATG 
               
               
                   
               
               
                 ATATATAATA TGATATGCTG TTAAGTTAGA GGTAAGATTC AATTTATTTA 
               
               
                   
               
               
                 AGATTATTTA ATTTGCGCTT TTATTATATA CATCTTCTAG TTGTAATTAA 
               
               
                   
               
               
                 TGGGGTAGAA TTAAAACTGT GTTTTGAATT AATATCGGTA TTAGAATAGG 
               
               
                   
               
               
                 ATTTGCATGA CTATATTTCT ACCTTACCAA GTTTTTTATC TGATAACACC 
               
               
                   
               
               
                 CTTTCACTTA TTATTTTCTT ATTTTTAATT TATTTAT 
               
               
                   
               
               
                 ATTTTTACCATATTCTATTTATACTAATCTTAAGTGGTACATTACCTCCCTGTG 
               
               
                   
               
               
                 GATACGACATATA 
               
               
                   
               
               
                 ATCTGTTTACTATCGTGACCGAAGTATAACTAATTGGGCGACATCACACCTATA 
               
               
                   
               
               
                 TCCCACAAACTAGCTACTATAGTCAATTTCTTGTTTTTTTTCCAATAGCCAATT 
               
               
                   
               
               
                 TTAAATGATGCCAAACTATTCAATGTATAATGTACATTTATTTTAAATAAGGGC 
               
               
                   
               
               
                 TTCACCTAACAAATGTGCCTAATTTTAGTTAATTTATTTTTTTATCGCATCTTA 
               
               
                   
               
               
                 CTATTTAAAGGAACTATCAAATTATAAAATATTTATGTCAATTCATTTGCCCCA 
               
               
                   
               
               
                 ACTCCAATATATAATATTATAAATAGGATAGTTCTCTATTCATAATAATTCAAA 
               
               
                   
               
               
                 ATATCATTAGGACTGAAGAAAA 
               
               
                   
               
               
                 (1635 bp) 
               
               
                 CBCA Synthase (CBCAS) ORF 
               
               
                 SEQ ID NO: 30 
               
            
           
           
               
               
            
               
                 1 
                 atgaattgct caacattctc cttttggttt gtttgcaaaa taatattttt ctttctctca 
               
               
                   
               
               
                 61 
                 ttcaatatcc aaatttcaat agctaatcct caagaaaact tccttaaatg cttctcggaa 
               
               
                   
               
               
                 121 
                 tatattccta acaatccagc aaatccaaaa ttcatataca ctcaacacga ccaattgtat 
               
               
                   
               
               
                 181 
                 atgtctgtcc tgaattcgac aatacaaaat cttagattca cctctgatac aaccccaaaa 
               
               
                   
               
               
                 241 
                 ccactcgtta ttgtcactcc ttcaaatgtc tcccatatcc aggccagtat tctctgctcc 
               
               
                   
               
               
                 301 
                 aagaaagttg gtttgcagat tcgaactcga agcggtggcc atgatgctga gggtttgtcc 
               
               
                   
               
               
                 361 
                 tacatatctc aagtcccatt tgctatagta gacttgagaa acatgcatac ggtcaaagta 
               
               
                   
               
               
                 421 
                 gatattcata gccaaactgc gtgggttgaa gccggagcta cccttggaga agtttattat 
               
               
                   
               
               
                 481 
                 tggatcaatg agatgaatga gaattttagt tttcctggtg ggtattgccc tactgttggc 
               
               
                   
               
               
                 541 
                 gtaggtggac actttagtgg aggaggctat ggagcattga tgcgaaatta tggccttgcg 
               
               
                   
               
               
                 601 
                 gctgataata tcattgatgc acacttagtc aatgttgatg gaaaagttct agatcgaaaa 
               
               
                   
               
               
                 661 
                 tccatgggag aagatctatt ttgggctata cgtggtggag gaggagaaaa ctttggaatc 
               
               
                   
               
               
                 721 
                 attgcagcat gtaaaatcaa acttgttgtt gtcccatcaa aggctactat attcagtgtt 
               
               
                   
               
               
                 781 
                 aaaaagaaca tggagataca tgggcttgtc aagttattta acaaatggca aaatattgct 
               
               
                   
               
               
                 841 
                 tacaagtatg acaaagattt aatgctcacg actcacttca gaactaggaa tattacagat 
               
               
                   
               
               
                 901 
                 aatcatggga agaataagac tacagtacat ggttacttct cttccatttt tcttggtgga 
               
               
                   
               
               
                 961 
                 gtggatagtc tagttgactt gatgaacaag agctttcctg agttgggtat taaaaaaact 
               
               
                   
               
               
                 1021 
                 gattgcaaag aattgagctg gattgataca accatcttct acagtggtgt tgtaaattac 
               
               
                   
               
               
                 1081 
                 aacactgcta attttaaaaa ggaaattttg cttgatagat cagctgggaa gaagacggct 
               
               
                   
               
               
                 1141 
                 ttctcaatta agttagacta tgttaagaaa ctaatacctg aaactgcaat ggtcaaaatt 
               
               
                   
               
               
                 1201 
                 ttggaaaaat tatatgaaga agaggtagga gttgggatgt atgtgttgta cccttacggt 
               
               
                   
               
               
                 1261 
                 ggtataatgg atgagatttc agaatcagca attccattcc ctcatcgagc tggaataatg 
               
               
                   
               
               
                 1321 
                 tatgaacttt ggtacactgc tacctgggag aagcaagaag ataacgaaaa gcatataaac 
               
               
                   
               
               
                 1381 
                 tgggttcgaa gtgtttataa tttcacaact ccttatgtgt cccaaaatcc aagattggcg 
               
               
                   
               
               
                 1441 
                 tatctcaatt atagggacct tgatttagga aaaactaatc ctgagagtcc taataattac  
               
               
                   
               
               
                 1501 
                 acacaagcac gtatttgggg tgaaaagtat tttggtaaaa attttaacag gttagttaag 
               
               
                   
               
               
                 1561 
                 gtgaaaacca aagctgatcc caataatttt tttagaaacg aacaaagtat cccacctctt 
               
               
                   
               
               
                 1621 
                 ccaccgcgtc atcat 
               
               
                   
               
            
           
           
               
            
               
                 (171 bp) 
               
               
                 CANON fragment 
               
               
                 SEQ ID NO: 31 
               
               
                 atgatgccaaactattcaatgtacaatgtacatttatttttaataagggcttcacctaacaa 
               
               
                   
               
               
                 aggtgcctaatttttgtgaacttttttttaccacatgtgactatttaatgactatcaaatta 
               
               
                   
               
               
                 taaaatatttaagtcaatttctttgcccccactccaatatataatgt 
               
               
                   
               
               
                 (232 bp) 
               
               
                 CANON fragment with putative TATA Box, 5′UTR, and start codon 
               
               
                 SEQ ID NO: 32 
               
               
                 atgatgccaaactattcaatgtacaatgtacatttatttttaataagggcttcacctaacaa 
               
               
                   
               
               
                 agqtgcctaatttttgtgaacttttttttaccacatgtqactatttaatgactatcaaatta 
               
               
                   
               
               
                 taaaatatttaagtcaatttctttgcccccactccaatatataatgt   tataaata   ggataat 
               
               
                   
               
               
                 tctcaattcatagtaattcaaaaatcattaggactaaagaaaa   atg     
               
               
                   
               
               
                 (709 bp) 
               
               
                 4 × CANON fragment synthetic promoter 
               
               
                 SEQ ID NO: 33 
               
               
                 
                   atgatgccaaactattcaatgtacaatgtacatttatttttaataagggcttcacctaacaa 
                 
               
               
                   
               
               
                 
                   aggtgcctaatttttgtgaacttttttttaccacatgtgactatttaatgactatcaaatta 
                 
               
               
                   
               
               
                 
                   taaaatatttaagtcaatttctttgcccccactcc 
                   atgatgccaaactattcaatgtacaat 
                 
               
               
                   
               
               
                 
                   gtacatttatttttaataagggcttcacctaacaaaggtgcctaatttttgtgaactttttt 
                 
               
               
                   
               
               
                 
                   ttaccacatgtgactatttaatgactatcaaattataaaatatttaagtcaatttctttgcc 
                 
               
               
                   
               
               
                 
                   cccactcc 
                   atgatgccaaactattcaatgtacaatgtacatttatttttaataagggcttca 
                 
               
               
                   
               
               
                 
                   cctaacaaaggtgcctaatttttgtgaacttttttttaccacatgtgactatttaatgacta 
                 
               
               
                   
               
               
                 
                   tcaaattataaaatatttaagtcaatttctttgcccccactcc 
                   atgatgccaaactattcaa 
                 
               
               
                   
               
               
                 
                   tgtacaatgtacatttatttttaataagggcttcacctaacaaaggtgcctaatttttgtga 
                 
               
               
                   
               
               
                 
                   acttttttttaccacatgtgactatttaatgactatcaaattataaaatatttaagtcaatt 
                 
               
               
                   
               
               
                   tctttgcccccactcc aatatataatgttataaataggataattctcaattcatagtaattc 
               
               
                   
               
               
                 aaaaatcattaggactaaagaaaaatg