Patent Publication Number: US-2023144097-A1

Title: Active dna transposon systems and methods for use thereof

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application claims priority benefit of International Patent Application No. PCT/CN2020/082087 filed Mar. 30, 2020, the contents of which are incorporated herein by reference in their entirety. 
    
    
     SUBMISSION OF SEQUENCE LISTING ON ASCII TEXT FILE 
     The content of the following submission on ASCII text file is incorporated herein by reference in its entirety: a computer readable form (CRF) of the Sequence Listing (file name: 182452000641SEQLIST.txt, date recorded: Mar. 29, 2021, size: 244 KB). 
     FIELD 
     The present application relates generally to the field of genetics. More specifically, the present application relates to transposable elements and uses thereof. 
     BACKGROUND 
     Typical methods for introducing DNA into a cell include DNA condensing reagents such as calcium phosphate, polyethylene glycol, and the like, lipid-containing reagents, such as liposomes, multi-lamellar vesicles, and the like, as well as virus-mediated strategies. However, such methods can have limitation. For example, there are size constraints associated with DNA condensing reagents and virus-mediated strategies. Further, the amount of nucleic acid that can be transfected into a cell is limited in virus strategies. Not all methods facilitate insertion of the delivered nucleic acid into cellular nucleic acid and while DNA condensing methods and lipid-containing reagents are relatively easy to prepare, the insertion of nucleic acid into viral vectors can be labor intensive. Virus-mediated strategies can be cell-type or tissue-type specific and the use of virus-mediated strategies can create immunologic problems when used in vivo. 
     One suitable tool in order to overcome these problems is by means of transposable elements. A transposable element (TE, transposon, or jumping gene) is a DNA sequence that can change its position in a nucleic acid, thereby creating or reversing mutations and altering sequences in a genome. Transposable elements represent a substantial fraction of many eukaryotic genomes. For example, around 50% of the human genome is derived from transposable element sequences and other genomes, for example, plants, may consist of substantially higher proportions of transposable element-derived DNA. Transposable elements are typically divided into two classes, class 1 and class 2. Class 1 is represented by the retrotransposons, including 1) long terminal repeat (LTR) retrotransposons, such as endogenous retroviruses (ERVs), and 2) non-LTR retrotransposons, such as long interspersed elements (LINEs) and short interspersed elements (SINEs). Class 2 TEs include 1) “cut-and-paste” DNA transposons, which are characterized by the terminal repeats (TRs, also known as terminal inverted repeats, TIRs) and are mobilized by a transposase, and 2) non-“cut-and-paste” DNA transposons, such as helitrons and polintons. While class 2 TEs are widespread and active in a variety of eukaryotes, not all of them are transpositionally active. Examples of recently active transposons include members of the hAT and piggyBac superfamilies with signs of mobilization in the past few million years. However, current options of active transposable elements available and suitable for gene discovery research and gene therapy are limited. 
     Therefore, there exists a need for new transposable elements suitable for introducing DNA into a cell, as well as methods and systems for efficient insertion of heterologous sequences of varying sizes into the nucleic acid of a cell or the insertion of DNA into the genome of a cell by means of transposable elements. 
     BRIEF SUMMARY 
     The present application provides engineered transposable elements, gene transfer systems comprising the engineered transposable elements, as well as methods and kits for using the same. Also provided are methods of inserting a heterologous nucleic acid into a target nucleic acid in vitro or in a cell. The compositions, systems and methods described herein are useful for various applications including tagmentation, genome engineering and gene discovery research. 
     In one aspect, the present application provides an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof, and wherein the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell. In some embodiments, the 5′TR comprises a nucleic acid sequence that has at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90. In some embodiments, the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90. In some embodiments, the 3′TR comprises a nucleic acid sequence that has at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102. In some further embodiments, the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102. 
     In some embodiments according to any one of the engineered transposable elements described above, the transposable element further comprises a 5′ target site duplication sequence (TSD) flanking the 5′ of the 5′TR or a 3′TSD flanking the 3′ of the 3′TR. In some embodiments, the nucleic acid sequences of 5′TSD and 3′TSD are the same sequence. In some embodiments, the 5′TSD comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 191-206, a variant thereof, or a fragment thereof, and the 3′TSD comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 191-206, a variant thereof, or a fragment thereof. 
     In some embodiments according to any one of the engineered transposable elements described above, the 5′TR comprises a nucleic acid sequence that has at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 3, 8, 11, 12, 13, 16, 22, 23, 29 and 82, and the 3′TR comprises a nucleic acid sequence that has at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 29, 34, 37, 38, 39, 42, 48, 49, 91 and 94. 
     In some embodiments according to any one of the engineered transposable elements described above, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, Tc1-1_AG, Tc1-1_PM, Tc1-4_Xt, Tc1-15_Xt, Mariner-6_AMi, or Mariner-3_Crp. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 3, and the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 29. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 8, and the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 34. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 11, and the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 37. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 12, and the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 38. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 16, and the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 42. 
     In some embodiments according to any one of the engineered transposable elements described above, the heterologous nucleic acid comprises a coding sequence. In some further embodiments, the heterologous nucleic acid further comprises a promoter operably linked to the coding sequence. 
     In some embodiments according to any one of the engineered transposable elements described above, the transposition activity of the transposable element is higher than that of a piggyBac (PB) transposon, a Sleeping Beauty (SB) transposon, and/or a TcBuster transposon. 
     In some embodiments according to any one of the engineered transposable elements described above, the cell is an animal cell, a plant cell, an algal cell, a fungal cell, a yeast cell, or a bacterial cell. In some embodiments, the cell is a mammalian cell. In some embodiments, the mammalian cell is selected from the group consisting of an immune cell (e.g., T cell), a hepatic cell, a tumor cell, a stem cell, a zygote, a muscle cell, and a skin cell. In some embodiments, the cell is a human cell. In some embodiments, the transposition activity of the transposable element is higher in a human embryonic kidney 293T (293T) cell than in a HeLa cell. 
     In some embodiments according to any one of the engineered transposable elements described above, the transposable element is present in a vector. In some further embodiments, the vector is a plasmid or a viral vector. 
     Another aspect of the present application provides a gene transfer system comprising: 1) an engineered transposable element according to any one of transposable elements described above; and 2) a transposase, or a nucleic acid encoding a transposase. In some embodiments, the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. 
     In yet another aspect, the present application provides a gene transfer system comprising: 1) an engineered transposable element; and 2) a transposase, or a nucleic acid encoding a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell, and wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, and wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof. 
     In some embodiments according to any one of the gene transfer systems described above, the transposable element comprises a 5′TR having a nucleic acid sequence that has at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 3, 8, 11, 12, 13, 16, 22, 23, 29 and 82, and the 3′TR comprises a nucleic acid sequence that has at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 29, 34, 37, 38, 39, 42, 48, 49, 91 and 94. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, Tc1-1_AG, Tc1-1_PM, Tc1-4_Xt, Tc1-15_Xt, Mariner-6_AMi, or Mariner-3_Crp. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 3, the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 29, and the transposase comprises the amino acid sequence of SEQ ID NO: 55. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 8, the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 34, and the transposase comprises the amino acid sequence of SEQ ID NO: 60. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 11, the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 37, and the transposase comprises the amino acid sequence of SEQ ID NO:63. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 12, the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 38, and the transposase comprises the amino acid sequence of SEQ ID NO: 64. In some embodiments, the 5′TR comprises a nucleic acid sequence of SEQ ID NO: 16, the 3′TR comprises a nucleic acid sequence of SEQ ID NO: 42, the transposase comprises the amino acid sequence of SEQ ID NO: 68. 
     In some embodiments according to any one of the gene transfer systems described above, the gene transfer system comprises a nucleic acid encoding the transposase. In some further embodiments, the transposable element and the nucleic acid encoding the transposase are in separate vectors. In some further particular embodiments, the transposable element and the nucleic acid encoding the transposase are in the same vector. 
     In still another aspect, the present application provides a method of inserting a heterologous nucleic acid into a target nucleic acid, comprising: contacting the target nucleic acid with a transposable element according to any one of the engineered transposable elements described above or a gene transfer system according to any one of the gene transfer systems describe above. In some embodiments, the method is carried out in vitro. In some embodiments, the target nucleic acid is in a cell. In some embodiments, the target nucleic acid is genomic DNA. 
     In some embodiments according to any one of the methods described above, wherein the target nucleic acid is in a cell, the cell is an animal cell, a plant cell, an algal cell, a fungal cell, a yeast cell, or a bacterial cell. In some embodiments, the cell is a mammalian cell. In some embodiments, the mammalian cell is selected from the group consisting of an immune cell (e.g., T cell), a hepatic cell, a tumor cell, a stem cell, a zygote, a muscle cell, and a skin cell. In some embodiments, insertion of the heterologous nucleic acid inactivates a gene of the cell. 
     In some embodiments according to any one of the methods described above, the heterologous nucleic acid encodes a protein. In some embodiments, the protein is selected from the group consisting of a reporter protein, an engineered receptor, a cytokine, an antibiotic resistance protein, an antigen, and a therapeutic protein. 
     In some embodiments according to any one of the methods described above, the heterologous nucleic acid encodes a RNA. In some embodiments, the RNA is selected from the group consisting of a therapeutic RNA, a small interfering RNA (siRNA), a microRNA, a short hairpin RNA (shRNA), a long non-coding RNA (lincRNA), and a guide RNA (gRNA). In some embodiments, the heterologous nucleic acid encodes more than one molecule. 
     In some embodiments according to any one of the methods described above, the heterologous nucleic acid is no more than about 300 kilobases (kb) long, e.g., about 10 kb to about 300 kb, or about 100 basepairs (bp) to about 10 kb, or about 100 bp to about 5 kb, or about 100 bp to about 2 kb, or about 2 kb to about 300 kb long. 
     In some embodiments according to any one of the methods described above, the insertion is random. 
     In still another aspect, the present application provides a kit comprising a transposable element according to any one of the engineered transposable elements described above, or a gene transfer system according to any one of the gene transfer systems described above and instructions for inserting a heterologous nucleic acid in a target nucleic acid. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG.  1    shows a diagram illustrating the process of identifying active transposable elements (TEs). 
         FIG.  2    shows a summary of the identified 131 transposable elements (TEs) harboring an open reading frame (ORF) of no less than 300 amino acid (aa) long, a transposase (Tn) domain, and an average divergence value of no more than 25%. The pie chart illustrates the distribution of the five superfamilies for the identified 131 TEs. 
         FIG.  3    shows an exemplary set of binary constructs for use in screening active transposable elements. The top construct is the helper construct for transposase expression, comprising from 5′ to 3′: a cytomegalovirus (CMV) promoter, a transposase (Tn) gene, and a polyA (pA) signal. The bottom construct is the donor construct, comprising from 5′ to 3′: a phosphoglycerate kinase (PGK) promoter, a 5′ target site duplication (5′TSD) sequence, a 5′ terminal repeat (5′TR) sequence, a sequence encoding puromycin and enhanced GFP fusion (Puro-eGFP), a 3′ terminal repeat (3′TR) sequence, a 3′ target site duplication (3′TSD) sequence, and a polyA (pA) signal. 
         FIGS.  4 A- 4 B  demonstrate the transposition activity of the identified transposable elements with a divergence value no greater than 5% in HEK293T (293T) and HeLa cells as compared with control TEs including piggyBac, Hyper piggyBac and SB100X.  FIG.  4 A  demonstrate the transposition activity of the identified transposable elements: 1_hAT-2_AG, 2_AgaP12, 3_P3_AG, 4_HAT2_CI, 5_HAT5_CI, 6_hAT-6_DR, 7_Chaplin1_DR, 8_Harbinger-4_XT, 9_HAT)_AG, 10_AgaP15, 11_IS4EU-1_DR, 12_POGO, 14_Tc1-8B_DR, 15_HOBO, 17_hAT-6_PM, 18_MARISP1, 19_hAT-7_XT, 20_BARI_DM, 21_Mariner-3_PM, 22_hAT-3_XT, 23_P1_AG, 24_IS4EU-2_DR, 25_Tc1-3_Xt, 26_Mariner-1_PM, 27_P2_AG, 28 hAT-5_DR, 29_Tc1-3_FR, 30_Mariner-4_XT 32_Tc1-5_Xt, 33_Tc1-10_Xt, and 34_hAT-1_D in HEK293T (293T) and HeLa cells as compared with control TEs including piggyBac, Hyper piggyBac, SB100X, TcBuster (TB), and negative control (NC).  FIG.  4 B  demonstrate the transposition activity of the identified transposable elements: 35_Mariner2_AG, 36_Tc1-1_Xt, 37_Tc1-1_AG, 38_Mariner-1_XT, 39_Tc1DR3_Xt, 40_hAT-1B_PM, 41_hAT-3_PM, 42_hAT-6B_PM, 43_Tc1-1_PM, 44_TC1_XL, 45_Mariner-4_AMi, 46_Motis_hAT1, 47_hAT-7_PM, 48_TC1_FR4, 49_hAT-8_PM, 50_piggyBac1_Mm, 51_Tc1-11_Xt, 52_Tc1-16_Xt, 53_TC1-2_DM, 54_Tc1-4_Xt, 55_TC1_DM, 56_Tc1-15_Xt, 59_TC1_FR2, 60_Mariner-6B_AMi, 61_Tc1-9_Xt, 63_hAT-9_XT, 64_Mariner-5_XT, 65_S2_DM, 67_PROTOP, 68_Tc1-8_Xt and 69_Tc1-12 Xt in HEK293T (293T) and HeLa cells as compared with control TEs including piggyBac, Hyper piggyBac, SB100X, TcBuster (TB), and negative control (NC). 
         FIGS.  5 A- 5 B  demonstrate the transposition activity of the identified transposable elements with a divergence value greater than 5% in HEK293T (293T).  FIG.  5 A  demonstrate the transposition activity of the identified transposable elements 70_Mariner-6 AMi, 71_hAT-3_Gav, 72_piggyBac2_Mm, 73_Mariner-2_XT, 74_hAT-4_Crp, 75_OposCharlie2, 76_hAT-1_AMi, 77_Mariner-2_AMi, 78_hAT-13_AMi, 80_hAT-12_AMi, 81_Mariner-7_Croc, 82_piggyBac-2_XT, 83_Mariner-3_AMi, 84_piggyBac1_CI, 86_piggyBac1-1_AMi, 87_Mariner-5_AMi, 89_Mariner-6_Crp, 90_Mariner-3_Crp, 91_hAT-3_AMi, 92_TC1_FR1, 93_hAT-5_Croc, 94_hAT-8_AMi, 95_PARIS, 96_Mariner-2_PM, 97_piggyBac-1_XT, 98_Tigger1, 99_Mariner-1_Cp, 101_S_DM, 102_hAT-12_Crp, 103_hAT-1_PM, 104_Senkusha1, 105_Tc1-2 PM, 106 Harbinger-2_AMi, 108_hAT-2_Gav and 110_Tigger2f in HEK293T (293T)  FIG.  5 B  demonstrate the transposition activity of the identified transposable elements 111_Tc1-4_DR, 116_Tigger17, 117_Tigger3, 118_hAT-14_Crp, 119_Mariner-9_Crp, 124_hAT-11_AMi, 126_Mariner-10_Crp, 139_hAT-17_Croc, 140_hAT-4_AMi, 142_Tigger4, 144_Tigger7, 156_Tigger2, 180_hAT-19_Crp, 183_hAT-17B_Croc, 188_Tc1-13_Xt, 197_hAT-19B_Croc, 199_MarsTigger8, 212_Tigger5, 222_hAT-10_XT 237_hAT-6_AMi, 245_MarsTigger1c, 246_Tigger17c, 258 Harbinger-1_AMi, 260_Tc1-14_Xt, 271 Kanga1, 275_Arthur1, 295_Harbinger-1_Crp, 314_Zaphod3, 320 Joey1, 331_Zaphod, 342_Harbinger-3_AMi, 348_Harbinger-1B_Crp, 349_MARWOLEN1 and 351_Zaphod2 in HEK293T (293T) 
         FIGS.  6 A- 6 B  show the transposition (i.e., transfer) efficiency of the various identified TEs as compared with control TEs piggyBac, hyperpiggyBac and SB100X.  FIG.  6 A  shows the transposition (transfer) efficiency of the various identified TEs as compared with control TEs piggyBac, hyperpiggyBac and SB100X in HEK293T (293T) cells.  FIG.  6 B  shows the transposition (transfer) efficiencies of the various identified TEs as compared with control TEs piggyBac, hyperpiggyBac and SB100X in HeLa cells. 
         FIGS.  7 A- 7 C  shows a phylogenetic tree illustrating the phylogenetic relationship among the identified transposases belonging to different superfamilies and their control TEs.  FIG.  7 A  shows a phylogenetic tree illustrating the phylogenetic relationship between the 14 identified hAT superfamily transposases 103_hAT-1_PM, 17_hAT-6_PM, 46 Myotis_hAT1, 28_hAT-5_DR, 22_hAT-3_XT, 41_hAT-3_PM, 47_hAT-7_PM, 180_hAT-19_Crp, 1_hAT-2_AG, 9_HAT)_AG, 139_hAT-17_Croc, 183_hAT-17B_Croc, 63_hAT-9 XT, 222_hAT-10_XT, and control TE TcBuster.  FIG.  7 B  shows a phylogenetic tree illustrating the phylogenetic relationship between the 2 identified piggyBac superfamily transposases 82_piggyBac-2_XT: 86_piggyBac-1_AMi and control TE piggyBac.  FIG.  7 C  shows a phylogenetic tree illustrating the phylogenetic relationship between the 22 identified TcMariner superfamily transposases and control TE SB100X. 
         FIGS.  8 A- 8 B  demonstrate the transposition activity of the identified transposable elements and top 5 active TEs among 131 candidates in HEK293T (293T) cells and HeLa cells as compared with control TEs including piggyBac, hyperpiggyBac and SB100X, and negative control (NC).  FIG.  8 A  shows transposition (transfer) efficiencies of the various identified TEs as compared with control TEs including piggyBac, hyperpiggyBac and SB100X, and negative control (NC) in 293T cells.  FIG.  8 B  shows transposition (transfer) efficiencies of the various identified TEs as compared with control TEs including piggyBac, hyperpiggyBac and SB100X, and negative control (NC) in HeLa cells. 
         FIGS.  9 A- 9 B  demonstrate the transposition activity of the identified transposable elements and top 5 active TEs among 131 candidates in Hct116 and K562 cells as compared with control TEs including piggyBac, hyperpiggyBac and SB100X, and negative control (NC).  FIG.  9 A  shows transposition (transfer) efficiencies of the various identified TEs as compared with control TEs including piggyBac, hyperpiggyBac and SB100X, and negative control (NC) in Hct116 cells.  FIG.  9 B  shows transposition (transfer) efficiencies of the various identified TEs as compared with control TEs including piggyBac, hyperpiggyBac and SB100X, and negative control (NC) in K562 cells. 
         FIG.  10 A- 10 B  demonstrate the transposition activity of the identified transposable elements 14-Tc1-8B_DR, 29-Tc1-3 FR, 35-Mariner2_AG, 36-Tc1-1_Xt, 37-Tc1-1_AG, 43-Tc1-1_PM, 52-Tc1-16_Xt, 54-Tc1-4_Xt, and 56-Tc1-15_Xt in primary T cells as compared with control TE SB100X.  FIG.  10 A  shows the transposition assay results of primary T cells transfected with plasmids containing EF1α promoter and CopGFP gene, wherein presence of GFP positive T cells represent successful transposition in the cells.  FIG.  10 B  shows the transposition assay results of primary T cells transfected with plasmids containing EF1α promoter and 019CAR-P2A-eGFP gene, wherein presence of GFP positive T cells represent successful transposition in the cells. 
         FIGS.  11 A- 11 B  demonstrate the transposition activity of the identified transposable elements SB100X and top 5 active TEs among 131 candidates based on different ratio of helper and transposon plasmids in 293T cells and HeLa cells as compared with control TE piggyBac and negative control (NC).  FIG.  11 A  shows transposition (transfer) efficiencies of the various identified TEs as compared with control TE SB100X and negative control (NC) in 293T cells.  FIG.  11 B  shows transposition (transfer) efficiencies of the various identified TEs as compared with control TE SB1001X and negative control (NC) in HeLa cells. 
         FIG.  12    demonstrate the cargo capacity of the identified transposable elements SB100X and top 5 active TEs among 131 candidates in 293T cells as compared with control TE piggyBac, piggyBac, hyperpiggyBac, SB100X.  FIG.  12    shows transposition (transfer) efficiencies of the various identified TEs as compared with control TEs piggyBac, piggyBac, hyperpiggyBac, SB100X. 
         FIG.  13    demonstrate the consensus sequences at the genomic insertion loci in a 40 bp window around the target site in genome from TEs original species and stable transposition K562 cell line. 
         FIGS.  14 A- 14 B  demonstrate the insertion frequencies of the identified transposable elements including piggyBac, hyperpiggyBac, SB100X and top 5 active TEs among 131 candidates in the distance to nearest gene,  FIG.  14 A  show the insertion frequencies in 0 bp-1 Mb windows and  FIG.  14 B  show the fold enrichment than random insertion in 50 kb windows. 
         FIG.  15    demonstrate the fold enrichment than random insertion of the identified transposable elements including piggyBac, hyperpiggyBac, SB100X and top 5 active TEs among 131 candidates in the relative location in gene body from 5′ terminus to 3′terminus. 
         FIG.  16    demonstrate the fold enrichment than random insertion of the identified transposable elements including piggyBac, hyperpiggyBac, SB100X and top 5 active TEs among 131 candidates in the different genes with expression level, level 7 means the highest expression and level 0 means the lowest expression. 
         FIG.  17    demonstrate the fold enrichment than random insertion of the identified transposable elements including piggyBac, hyperpiggyBac, SB100X and top 5 active TEs among 131 candidates in the location around transcription start sites (TSS) in 5 kb windows. 
         FIG.  18    demonstrate the fold enrichment than random insertion of the identified transposable elements including piggyBac, hyperpiggyBac, SB100X and top 5 active TEs among 131 candidates in the different chromatin states. 
     
    
    
     DETAILED DESCRIPTION 
     The present application provides engineered transposable elements, gene transfer systems comprising the engineered transposable elements, as well as methods and kits for using the same. The present application is based, at least in part, on the identification of novel transposable elements (e.g., Table 2) from a wide range of species from pan-genome bioinformatics analysis, as well as the surprising results that a number of the identified transposable elements are capable of transposition in human cells with high efficiency. The disclosed compositions, systems, and methods are useful for inserting heterologous nucleic acids into a target nucleic acid, including introducing heterologous DNA into the genome of a cell. The transposable elements and gene transfer systems described herein can be used for various applications, such as gene therapy and gene discovery research. 
     Accordingly, in one aspect, the present application provides an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof, and wherein the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell. 
     In another aspect, the present application provides a gene transfer system comprising: 1) an engineered transposable element; and 2) a transposase, or a nucleic acid encoding a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell. In some embodiments, the transposable element is an engineered transposable element. In some embodiments, the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. 
     I. Definitions 
     As used herein, the term “transposon,” “transposable element” or “TE” refers to a polynucleotide that is able to excise from a first nucleic acid (i.e., donor nucleic acid, for example, a vector) and integrate into a target site (e.g., a second nucleic acid or genomic or extrachromosomal DNA in a cell). A transposon includes a nucleic acid sequence flanked by cis-acting nucleic acid sequences on the termini of the transposon. A nucleic acid sequence is “flanked by” cis-acting nucleic acid sequences if at least one cis-acting nucleic acid sequence is positioned 5′ to the nucleic acid sequence, and at least one cis-acting nucleic acid sequence is positioned 3′ to the nucleic acid sequence. Cis-acting nucleic acid sequences include at least one terminal repeat (TR, also known as an inverted terminal repeat (ITR) or a terminal inverted repeat (TIR)) at each end of the transposon, to which a transposase binds. A transposable element described herein may or may not contain an open reading frame (ORF) encoding a transposase. 
     As used herein, the term “transposition” refers to the change in location of a transposable element from a first nucleic acid (e.g., a vector) and integrate into a target site (e.g., a second nucleic acid or genomic or extrachromosomal DNA in a cell). 
     As used herein, the term “terminal repeats” or “TRs” refer to nucleic acid sequences at both ends of a transposable element and flanking a second nucleic acid sequence that can be transposed. The TR located 5′ (upstream) to the second nucleic acid sequence is referred to the 5′TR, and the TR located 3′ (downstream) to the second nucleic acid sequence is referred to the 3′TR. In class 2 transposable elements, the TRs are complements to each other. 
     As used herein, the term “target site duplication” or “TSD” refers to nucleic acid sequences that occur at insertion sites of transposable elements. TSDs may occur due to DNA repair of sticky ends caused by staggered cut of target DNA duplex by transposases. TSDs flank TRs in a transposable element. The TSD located 5′ to the 5′TR is the 5′TSD. The TSD located 3′ to the 3′TR is the 3′TSD. 
     As used herein, the term “transposase” refers to a polypeptide that catalyzes the excision of a transposon from a first nucleic acid (e.g., a vector) and integrate into a target site (e.g., a second nucleic acid or genomic or extrachromosomal DNA in a cell). In some embodiments, a transposase binds one or both terminal repeat sequences. 
     As used herein, a “left transposon fragment” or “LTF” refers to a fragment in a naturally occurring transposable element from the 5′TSD to the start codon of a transposase ORF sequence. As used herein, a “right transposon fragment” or “RTF” refers to a fragment in a naturally occurring transposable element from the stop codon of the transposase ORF sequence to the 3′TSD. 
     The terms “nucleic acid”, “polynucleotide”, and “nucleic acid sequence” are used interchangeably to refer to a polymeric form of nucleotides of any length, including deoxyribonucleotides, ribonucleotides, combinations thereof, and analogs thereof. “Oligonucleotide” and “oligo” are used interchangeably to refer to a short polynucleotide, having no more than about 50 nucleotides. 
     As used herein, a “heterologous nucleic acid” refers to a DNA or RNA sequence that is from a different origin than a reference nucleic acid sequence. For example, in the context of a transposable element, a heterologous nucleic acid is from a different origin than the terminal repeat sequences. For example, a nucleic acid sequence that has been isolated from an organism different from that of the terminal repeats is considered a heterologous nucleic acid with respect to the terminal repeats. 
     As used herein, the term “operably linked” refers to a nucleic acid sequence that is placed in a functional relationship with another nucleic acid sequence. For example, if a coding sequence is operably linked to a promoter sequence, this generally means that the promoter may promote transcription of the coding sequence. Operably linked means that the DNA sequences being linked are typically contiguous and, where necessary join two protein coding regions, contiguous and in reading frame. Since enhancers may function when separated from the promoter by several kilobases and intron sequences may be of variable lengths, some nucleic acid sequences may be operably linked but not contiguous. 
     “Percentage (%) sequence identity” with respect to a nucleic acid sequence is defined as the percentage of nucleotides in a candidate sequence that are identical with the nucleotides in the specific nucleic acid sequence, after aligning the sequences by allowing gaps, if necessary, to achieve the maximum percent sequence identity. “Percentage (%) sequence homology” with respect to a peptide, polypeptide or protein sequence is the percentage of amino acid residues in a candidate sequence that are identical substitutions to amino acid residues in the specific peptide or amino acid sequence, after aligning the sequences by allowing gaps, if necessary, to achieve the maximum percent sequence homology. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways that are within the skill in the art, for instance, using publicly available computer software such as BLAST, BLAST-2, ALIGN or MEGALIGN™ (DNASTAR) software. Those skilled in the art can determine appropriate parameters for measuring alignment, including any algorithms needed to achieve maximal alignment over the full length of the sequences being compared. 
     The term “vector” as used herein refers to a nucleic acid molecule capable of transporting another nucleic acid molecule to which it has been linked. Examples of vectors include but are not limited to bacteria, plasmids, phages, cosmids, episomes, viruses, and insertable DNA fragments, i.e., fragments capable of being inserted into a host cell genome by homologous recombination. 
     As used herein, the term “plasmid” refers to circular, double-stranded DNA capable of accepting a foreign DNA fragment and capable of replicating in prokaryotic or eukaryotic cells. 
     The terms “polypeptide”, and “peptide” are used interchangeably herein to refer to polymers of amino acids of any length. Thus, for example, the terms peptide, oligopeptide, protein, antibody, and enzyme are included within the definition of polypeptide. The polymer may be linear or branched, it may comprise modified amino acids, and it may be interrupted by non-amino acids. A protein may have one or more polypeptides. The terms also encompass an amino acid polymer that has been modified; for example, disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation, such as conjugation with a labeling component. 
     As used herein, a “variant” is interpreted to mean a polynucleotide or polypeptide that differs from a reference polynucleotide or polypeptide, respectively, but retains essential properties. A typical variant of a polynucleotide differs in nucleic acid sequence from another, reference polynucleotide. Changes in the nucleic acid sequence of the variant may or may not alter the amino acid sequence of a polypeptide encoded by the reference polynucleotide. Nucleotide changes may result in amino acid substitutions, additions, deletions, fusions and truncations in the polypeptide encoded by the reference sequence, as discussed below. A typical variant of a polypeptide differs in amino acid sequence from another, reference polypeptide. Generally, differences are limited so that the sequences of the reference polypeptide and the variant are closely similar overall and, in many regions, identical. A variant and reference polypeptide may differ in amino acid sequence by one or more substitutions, additions, deletions in any combination. A substituted or inserted amino acid residue may or may not be one encoded by the genetic code. A variant of a polynucleotide or polypeptide may be a naturally occurring such as an allelic variant, or it may be a variant that is not known to occur naturally. Non-naturally occurring variants of polynucleotides and polypeptides may be made by mutagenesis techniques, by direct synthesis, and by other recombinant methods known to skilled artisans. 
     As used herein, a “fragment” of a sequence refers to a portion of the sequence. For example, a fragment of a nucleic acid sequence refers to a portion of the nucleic acid sequence, and a fragment of an amino acid sequence refers to a portion of the amino acid sequence. 
     As used herein the term “genetic circuit”, “biological circuit”, or “synthetic circuit” refers to a set of biological components designed to perform logical functions. In general, an input is needed to activate a genetic circuit, which subsequently produces an output as a function of the input. 
     The term “engineer” as used herein refers to any manipulation that results in a detectable change in a polynucleotide or a polypeptide, wherein the manipulation includes, but is not limited to, inserting, deleting, and substituting a portion of the polynucleotide or amino acid sequence. 
     The term “transposition efficiency” as used herein refers to the efficiency of a transposable element to insert a heterologous nucleic acid into a population of target cells. For example, transposition efficiency can be determined by transfecting a plasmid comprising a transposable element comprising a reporter gene or a gene encoding a selectable marker, for example, an antibiotic resistance gene (e.g., puromycin), into a population of target cells, and determine the number of cells expressing the gene product encoded by the reporter gene or selectable marker, for example, by measuring the number of cells having antibiotic resistance. 
     The term “transfected” or “transformed” or “transduced” as used herein refers to a process by which exogenous nucleic acid is transferred or introduced into a host cell. A “transfected” or “transformed” or “transduced” cell is one, which has been transfected, transformed or transduced with exogenous nucleic acid. The term “transduction” and “transfection” as used herein include all methods known in the art using an infectious agent (such as a virus) or other means to introduce DNA into cells for expression of a protein or molecule of interest. Besides a virus or virus like agent, there are chemical-based transfection methods, such as those using calcium phosphate, dendrimers, liposomes, or cationic polymers (e.g., DEAE-dextran or polyethylenimine); non-chemical methods, such as electroporation, cell squeezing, sonoporation, optical transfection, impalefection, protoplast fusion, delivery of plasmids, or transposons; particle-based methods, such as using a gene gun, magnectofection or magnet assisted transfection, particle bombardment; and hybrid methods, such as nucleofection. 
     The term “in vivo” refers to inside the body of the organism from which the cell is obtained. “Ex vivo” or “in vitro” means outside the body of the organism from which the cell is obtained. 
     It is understood that embodiments of the invention described herein include “consisting” and/or “consisting essentially of” embodiments. 
     Reference to “about” a value or parameter herein includes (and describes) variations that are directed to that value or parameter per se. For example, description referring to “about X” includes description of “X”. 
     As used herein, reference to “not” a value or parameter generally means and describes “other than” a value or parameter. For example, the method is not used to treat cancer of type X means the method is used to treat cancer of types other than X. 
     The term “about X-Y” used herein has the same meaning as “about X to about Y.” 
     As used herein and in the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise. 
     II. Engineered Transposable Elements 
     The present application in one aspect provides an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR). In some embodiments, the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into a target nucleic acid (e.g., DNA) in vitro. In some embodiments, the engineered transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into a target nucleic acid in a cell (such as DNA in a plant of mammalian cell). In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     In some embodiments, the engineered transposable element comprises, from 5′ to 3′: a 5′ target site duplication sequence (5′TSD), a 5′TR, a heterologous nucleic acid, a 3′TR and a 3′TSD. In some embodiments, the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into a target nucleic acid (e.g., DNA) in vitro. In some embodiments, the engineered transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into a target nucleic acid in a cell (such as DNA in a plant of mammalian cell). In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     In some embodiments, the present application provides novel transposable elements from a wide range of species that are capable of transposition in human cells with high efficiency. The transposable elements described herein provide direct experimental evidence for naturally active mammalian cut-and-paste DNA transposons. 
     A list of exemplary transposable elements with their corresponding left terminal fragment (LTF), right terminal fragment (RTF), transposase, 5′terminal repeat (TR), 3′TR, 5′ target site duplication (TSD) and 3′TSD sequences can be found in Tables 1-2 and the sequence listing. Table 1 lists 131 TEs identified from the bioinformatics analysis as disclosed in the present application, including 11 TEs (TE IDs: 4, 5, 7, 12, 18, 20, 22, 25, 26, 28 and 30) meeting the criteria of having a length of no more than 3000 bp, a MITE copy number greater than 10, and an average divergence smaller than 1%, suitable for use in efficient genome engineering. Table 2 lists experimentally validated active TEs using transposition assays in human cell lines HEK293T and Hela. 
     In some embodiments, the transposable elements are Class 2 transposable elements. Class 2 transposable elements may be classified into superfamilies based on the relatedness of the transposase and on shared structural features, including the terminal repeats (TRs) and the length of the target site duplications (TSDs) generated during integration flanking the TRs. It is contemplated that the transposable element of the present application may be from various suitable TE superfamilies and/or families. In some embodiments, the transposable element is from the hAT superfamily. In some embodiments, the transposable element is from the P superfamily. In some embodiments, the transposable element is from the PIF-Harbinger superfamily. In some embodiments, the transposable element is from the piggyBac superfamily. In some embodiments, the transposable element is from the TcMariner superfamily. In some embodiments, the 5′TR is a reverse complement of the 3′ TR. In some embodiments, the 5′TR is not a reverse complement of the 3′TR. 
     In some embodiments, the engineered transposable element comprises a 5′TR comprising a nucleic acid sequence that has at least about 50%, 60%, 70%, 80%, 90%, 95%, 99% or 100% sequence identity to the 5′TR of a transposable element of a superfamily selected from the group consisting of hAT, P, PIF-Harbinger, piggyBac, and TcMariner. In some embodiments, the engineered transposable element comprises a 3′TR comprising a nucleic acid sequence that has at least about 50%, 60%, 70%, 80%, 90%, 95%, 99% or 100% sequence identity to the 3′TR of a transposable element of a superfamily selected from the group consisting of hAT, P, PIF-Harbinger, piggyBac, and TcMariner. 
     In some embodiments, the engineered transposable element comprises a 5′TR comprising a nucleic acid sequence that has at least about 50%, 60%, 70%, 80%, 90%, 95%, 99% or 100% sequence identity to the 5′TR of a transposable element of a superfamily selected from the group consisting of hAT, P, PIF-Harbinger, piggyBac, and Tc1, and a 3′TR comprising a nucleic acid sequence that has at least about 50%, 60%, 70%, 80%, 90%, 95%, 99% or 100% sequence identity to the 3′TR of a transposable element of a superfamily selected from the group consisting of hAT, P, PIF-Harbinger, piggyBac, and TcMariner. 
     In some embodiments, the engineered transposable element comprises a 5′ TR, a 3′TR, a LTF, an RTF, a transposase, a 5′TSD, and/or a 3′TSD derived from any one of the TEs of Table 1. In some embodiments, the engineered transposable element comprises a 5′ TR, a 3′TR, a LTF, an RTF, a transposase, a 5′TSD, and/or a 3′TSD derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, Tc1-1_AG, Tc1-1_PM, Tc1-4 Xt, Tc1-15_Xt, Mariner-6 AMi, or Mariner-3_Crp. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, the engineered transposable element comprises a LTF comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 115-140 and 167-178, a variant thereof, or a fragment thereof. 
     In some embodiments, the engineered transposable element comprises a RTF comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 141-190, a variant thereof, or a fragment thereof. 
     In some embodiments, the engineered transposable element comprises a LTF having at least about any one of 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 115-140 and 167-178; and a RTF having at least about any one of 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 141-190. In some embodiments, the engineered transposable element comprises a LTF comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 115-140 and 167-178; and an RTF comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 141-190. 
     In some embodiments, the engineered transposable element comprises a 5′TR in a LTF comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 115-140 and 167-178._In some embodiments, the engineered transposable element comprises a 3′TR in a RTF comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 141-190. 
     In some embodiments, the engineered transposable element comprises a 5′TR in a LTF comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 115-140 and 167-178, a variant of the 5′TR, or a fragment of the 5′TR; and a 3′TR in a RTF comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 141-190, a variant of the 3′TR, or a fragment of the 3′TR. 
     In some embodiments, the engineered transposable element comprises a 5′TR having a nucleic acid sequence that has at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90. In some embodiments, the engineered transposable element of the present application comprises a 5′TR having a nucleic acid sequence that has at least about any one of 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90. In some embodiments, the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90. In some embodiments, the engineered transposable element comprises a 3′TR that has complementary sequence as the 5′TR. 
     In some embodiments, the engineered transposable element comprises a 3′TR having a nucleic acid sequence that has at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102. In some embodiments, the engineered transposable element comprises a 3′TR having a nucleic acid sequence that has at least about any one of 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102. In some embodiments, the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102. In some embodiments, the engineered transposable element comprises a 5′TR that has complementary sequence as the 3′TR. 
     In some embodiments, the engineered transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g. at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102. 
     In some embodiments, the engineered transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52. 
     In some embodiments, the engineered transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 79-90; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 91-102. 
     Also contemplated herein are engineered transposable elements comprising a variant or a fragment of any one of the 5′TRs and/or 3′TRs, or LTF and/or RTF described herein, e.g., in Tables 1 and 2. In some embodiments, the variant comprises no more than about any one of 50, 40, 35, 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 nucleotide substitution(s). In some embodiments, the fragment comprises at least about any one of 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 150, 200, 250, 300, 350, 400, 450, or 500 nucleotides. 
     In some embodiments, the engineered transposable element comprises a 5′TSD. In some embodiments, the engineered transposable element comprises a 3′TSD. In some embodiments, the engineered transposable element comprises both a 5′TSD and a 3′TSD. In some embodiments, the engineered transposable element does not comprise a 5′TSD. In some embodiments, the engineered transposable element does not comprise a 3′TSD. In some embodiments, the engineered transposable element does not comprise a 5′TSD or a 3′TSD. In some embodiments, the 5′TSD is identical to the 3′TSD. In some embodiments, the 5′TSD is different from the 3′TSD. 
     In some embodiments, the engineered transposable element comprises a nucleic acid sequence encoding a transposase. In some embodiments, the engineered transposable element does not comprise a nucleic acid sequence encoding a transposase. In some embodiments, the transposase is derived from the same species as the 5′TR and 3′TR sequences. In some embodiments, the transposase is a native transposase with respect to the 5′TR and 3′TR sequences. In some embodiments, the transposase is an engineered transposase based on a native transposase with respect to the 5′TR and 3′TR sequences. 
     Transposase catalyzes excision of a transposon from a donor polynucleotide (e.g., a vector) and subsequent integration of the transposon into a target nucleic acid, such as genomic or extrachromosomal DNA of a target cell. In some embodiments, a transposase binds a terminal repeat of a transposable element. 
     In some embodiments, the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, a variant thereof, or a fragment thereof. 
     The present application in some embodiments contemplate variants of the transposases listed in Tables 1-2 and the sequence listing. The recitation of a transposase variant refers to transposase polypeptides that are distinguished from a reference transposase polypeptide (e.g., a naturally occurring transposase polypeptide) by the addition, deletion, truncations, and/or substitution of at least one amino acid residue, which retain transposition activity. In certain embodiments, a transposase polypeptide variant is distinguished from a reference transposase polypeptide by one or more substitutions, which may be conservative or non-conservative, as known in the art. In certain embodiments, a variant transposase comprises an amino acid sequence having at least about any one of 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity or similarity to a corresponding sequence of a reference transposase. In some embodiments, a variant transposase comprises no more than about any one of 50, 40, 35, 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 amino acid substitutions. 
     Functional fragments having amino acid deletions or variants having amino acid additions of the transposases described herein are also contemplated. In some embodiments, a transposase fragment is at least about any one of 50, 60, 70, 80, 90, 100, 150, 200, 250, 300, 350, 400, 450, 500, 600, 700 or more amino acid residues long. In certain embodiments, the amino acid additions or deletions occur at the C-terminal end and/or the N-terminal end of the reference transposase. In some embodiments, the amino acid additions or deletions occur at an internal position, such as a flexible loop of the reference transposase. In certain embodiments, the amino acid deletions (e.g., N-terminal and/or C-terminal truncation) comprises any one of about 1, about 2, about 3, about 4, about 5, about 6, about 7, about 8, about 9, about 10, about 15, about 20, about 25, about 30, about 35, about 40, about 45, 50, about 55, about 60, about 65, about 70, about 75, about 80, about 85, about 90, about 95, about 100, about 105, about 110, about 115, about 120, about 125, about 130, about 135, about 140, about 145, about 150, about 155, about 160, about 165, about 170, about 175 or more amino acids, including all values and ranges in between these values. In some embodiments, a variant transposase comprises an N-terminal or C-terminal purification tag, selection marker (e.g., antibiotic resistance gene), or a reporter (e.g., a fluorescence reporter). 
     As noted above, transposase polypeptides of the invention may be altered in various ways including amino acid substitutions, deletions, truncations, and insertions. Methods for such manipulations are generally known in the art. For example, amino acid sequence variants of a reference polypeptide can be prepared by mutations in the DNA. Methods for mutagenesis and nucleic acid sequence alterations are well known in the art. See, for example, Kunkel (1985 , Proc. Natl. Acad. Sci. USA.  82: 488-492), Kunkel et al., (1987 . Methods in Enzymol,  154: 367-382), U.S. Pat. No. 4,873,192, Watson, J. D. et al., ( Molecular Biology of the Gene , Fourth Edition, Benjamin/Cummings, Menlo Park, Calif., 1987) and the references cited therein. Guidance as to appropriate amino acid substitutions that do not affect biological activity of the protein of interest may be found in the model of Dayhoff et al., (1978)  Atlas of Protein Sequence and Structure  (Natl. Biomed. Res. Found., Washington, D.C.). 
     In some embodiments, the transposase is codon-optimized as compared to the reference transposase. In some embodiments, the transposase is codon-optimized for expression in a mammalian cell, such as a human cell. In some embodiments, the transposase is codon-optimized for expression in a plant cell. 
     In some embodiments, the transposase comprises an amino acid sequence that has at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to an amino acid sequence selected from the group consisting SEQ ID NOs: 53-78 and 103-114. In some embodiments, the transposase comprises an amino acid sequence selected from the group consisting SEQ ID NOs: 53-78 and 103-114. 
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 115; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 141 In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of TAGGC (SEQ ID NO: 1), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of GCCTA (SEQ ID NO: 27), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 1; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 27. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 1; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 27. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of GTATGGAC (SEQ ID NO: 191), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 191. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 115; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 141. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 53, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 53. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-2_AG) 
               
               
                   
                 SEQ ID NO: 53 
               
               
                   
                 MASNVATSSSIWDHFTRNGTKAKCRYCYNEIAFTK 
               
               
                   
                   
               
               
                   
                 GSTSNLKRHMKAKHPSISLERQHLASTTSPSTQNS 
               
               
                   
                   
               
               
                   
                 EPGPSCSSRPPNLISNFFKQPMNSETKRKLDMMLL 
               
               
                   
                   
               
               
                   
                 KLICKDCLPLSIVESEAFKTFVGCLNQNYDLPTRK 
               
               
                   
                   
               
               
                   
                 NVSNALLPSIYNEILVKVQGEVRNATSIALTTDGW 
               
               
                   
                   
               
               
                   
                 TNVNNTSFLGLTAHFIDNDYKLRSCLLECSEISLS 
               
               
                   
                   
               
               
                   
                 HSGQNIAAWIKEVIIKYEIQDKIVGIVTDNAANMK 
               
               
                   
                   
               
               
                   
                 SAARELEFNHVTCFAHSLHLIVKDATKKSIISTVD 
               
               
                   
                   
               
               
                   
                 EVKRIVMYFKKSPKATNELADTQSKFNLPNLKLKQ 
               
               
                   
                   
               
               
                   
                 DVPTRWNSTYDMLNRFYKNKIAIVACADKLNTLDP 
               
               
                   
                   
               
               
                   
                 IKIDWAILEHSLNALKIFDVATNMVSAEKNITVSH 
               
               
                   
                   
               
               
                   
                 VGLLSKMIIRKLNETDYTIPELGNLVTHLKEGVKK 
               
               
                   
                   
               
               
                   
                 RLEIYSNNQIIAKSMLLDPRIKKQGFHEEPLKYRE 
               
               
                   
                   
               
               
                   
                 TYELIIQELIPFQTPSMNAQEPHNIDNEANLLLGE 
               
               
                   
                   
               
               
                   
                 FITWVNNAECEIESPIELAKNELNSFLKIRNIDIK 
               
               
                   
                   
               
               
                   
                 NDPLEWWRIHSSKYPSIYALAKTIICIPGTSVPCE 
               
               
                   
                   
               
               
                   
                 RLFSKAGQIYSDKRSRLHPKKFKEIIFIQQNVDKF 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 116; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 142. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of TAG (SEQ ID NO: 2), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of CTA (SEQ ID NO: 28), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 2; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 28. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 2; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 28. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of ATGTGAAC (SEQ ID NO: 192), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 192. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 116; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 142. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 54, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 54. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of HAT1_AG) 
               
               
                   
                 SEQ ID NO: 54 
               
               
                   
                 MMAPTNATTSPVWDHFSPVETGAKCLYCLKVFKYT 
               
               
                   
                   
               
               
                   
                 KGTTSNLKRHLNLVHKTVPYLKQKQPIPQTINIDD 
               
               
                   
                   
               
               
                   
                 EAGPSAVNFQPSNQYFNSNMSIQGYLKKPINSETK 
               
               
                   
                   
               
               
                   
                 KVLDRMLLDLICKECLPFNLVESEIFKKFVYTLNP 
               
               
                   
                   
               
               
                   
                 NYIMPTRKSLSNALLPSVYNQEFEKAKEKLSTAKA 
               
               
                   
                   
               
               
                   
                 IAITSDGWTNLNQISFFALTGHYIDENCKLSSILI 
               
               
                   
                   
               
               
                   
                 ECSEFENPHSGRNIANWIQGTLNKFDIEDKIVAMV 
               
               
                   
                   
               
               
                   
                 TDNASNMKAASTELNFCHIPCFAHTLNLIVRDAIK 
               
               
                   
                   
               
               
                   
                 KSVLPVVEEVKRVVMLFKKSPKASQMLADTQKKLN 
               
               
                   
                   
               
               
                   
                 LDQLKMIQEVSTRWNSGYDMLNRFYKNKIALLSCA 
               
               
                   
                   
               
               
                   
                 DSLKMKISLESHDWEAIEQIVRVLKYFYSATNIVS 
               
               
                   
                   
               
               
                   
                 AQKYITISHVGLLCNVLLTKTSQFRNDEDIAENIQ 
               
               
                   
                   
               
               
                   
                 NLVALLIEGLQNKLKIYRSNEQILKSMILDPRIKQ 
               
               
                   
                   
               
               
                   
                 LGFQDDVEKFKNICESIISELLPLQKPAVEVEKVV 
               
               
                   
                   
               
               
                   
                 KKVSKDVDMLFGDLLKNKGAQNYKTPRQIAENELH 
               
               
                   
                   
               
               
                   
                 QYLSVENIDLENDPLLWWKEHQVLYPSLYTLAMST 
               
               
                   
                   
               
               
                   
                 LCIPGTSVPCERLFSKAGQIYSEKRSRLAPKKLQE 
               
               
                   
                   
               
               
                   
                 ILFIQQNA 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 117; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 143. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 3, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 29, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 3; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 29. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 3; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 29. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of TA (SEQ ID NO: 193), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 117; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 143. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 55, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 55. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-8B_DR) 
               
               
                   
                 SEQ ID NO: 3 
               
               
                   
                 CAGCGGGGAAAATAAGTATTTGACACATCAGCATT 
               
               
                   
                   
               
               
                   
                 TTTATCAGTAAGGGGATTTCTAAGTGGGCTACTGA 
               
               
                   
                   
               
               
                   
                 CACAAAATTCCTACCAGATGTAGCCATCAAGCCAA 
               
               
                   
                   
               
               
                   
                 ATATTGAATTCATACAAAGAAATCAGAACATTTAA 
               
               
                   
                   
               
               
                   
                 GTATACAAGTTGAGTCATAATAAATAAAGTGAAAT 
               
               
                   
                   
               
               
                   
                 GACACAGGGAATAAGTATTGAACACA 
               
               
                   
                   
               
               
                   
                 (3′TR of Tcl-8B_DR) 
               
               
                   
                 SEQ ID NO: 29 
               
               
                   
                 TGTGTTCAATACTTATTCCCTGTGTCATTTCACTT 
               
               
                   
                   
               
               
                   
                 TATTTATTATGACTCAACTTGTATACTTAAATGTT 
               
               
                   
                   
               
               
                   
                 CTGATTTCTTTGTATGAATTCAATATTTGGCTTGA 
               
               
                   
                   
               
               
                   
                 TGGCTACATCTGGTAGGAATTTTGTGTCAGTAGCC 
               
               
                   
                   
               
               
                   
                 CACTTAGAAATCCCCTTACTGATAAAAATGCTGAT 
               
               
                   
                   
               
               
                   
                 GTGTCAAATACTTATTTTCCCCGCTG 
               
               
                   
                   
               
               
                   
                 (transposase of Tc1-8B_DR) 
               
               
                   
                 SEQ ID NO: 55 
               
               
                   
                 MMGKNKELSQDLRSLIVEKHFDGNGYRRISRMLNV 
               
               
                   
                   
               
               
                   
                 PVSTVGAIIRKWKKHKFTINRPRSGAPRKIPVRGV 
               
               
                   
                   
               
               
                   
                 QRIIRRVLQEPRTTRAELQEDLASAGTIVSKKTIS 
               
               
                   
                   
               
               
                   
                 NALNHHGIHARSPRKTPLLNKKHVEARLKFAKQHL 
               
               
                   
                   
               
               
                   
                 EKPVDYWETIVWSDESKIELFGSHSTHHVWRRNGT 
               
               
                   
                   
               
               
                   
                 AHHPKNTIPTVKFGGGSIMVWGCFSARGTGRLHII 
               
               
                   
                   
               
               
                   
                 EGRMNGEMYRDILDKNLLPSTRKLKMKRGWTFQQD 
               
               
                   
                   
               
               
                   
                 NDPKHKAKETMKWFQRKKIKLLEWPSQSPDLNPIE 
               
               
                   
                   
               
               
                   
                 NLWRELKIKVHKRGPRNLQDLKTVCVEEWARJTPE 
               
               
                   
                   
               
               
                   
                 QCRRLVSPYKRRLEAVITNKGFSTKY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 118; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 144. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 4, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 30, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 4; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 30. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 4; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 30. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of TTAGAG (SEQ ID NO: 195), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 195. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 118; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 144. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 56, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 56. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-6_PM) 
               
               
                   
                 SEQ ID NO: 56 
               
               
                   
                 MAKRKKDDEYRTFQDEWTEEFAFVERAGSAVCLIC 
               
               
                   
                   
               
               
                   
                 NDKITSLKRSNVKRHFDTRHATFASKYPAGDSRKK 
               
               
                   
                   
               
               
                   
                 ACQELLSRVQASQQQLRVWTRQGDYNSASFAGSLA 
               
               
                   
                   
               
               
                   
                 IVRNGKPFTDGEYAKTFMLDVANELFDDLPNKDKI 
               
               
                   
                   
               
               
                   
                 IKRIQDMPLSARTVHDRTIVMANKVEETQVKDINA 
               
               
                   
                   
               
               
                   
                 APFFSLALDESTDVSHLSQFSVIARYAVGDTLREE 
               
               
                   
                   
               
               
                   
                 SLAVLPLKGSTRGEDLFKSFMEFAQEKSLPMDKLL 
               
               
                   
                   
               
               
                   
                 SVCTDGAPCMVGKNKGFVALLREHENRPILSFHCI 
               
               
                   
                   
               
               
                   
                 IIIQEALCAQMCDRQFGEVMSLVIRVINFIVARAL 
               
               
                   
                   
               
               
                   
                 NDRQFKTLLDEVVNNYPGLLLHSNVRWLSRGKVLS 
               
               
                   
                   
               
               
                   
                 RFAACLNEIRTFLEMKGVGHPELADTEWLLKFYYL 
               
               
                   
                   
               
               
                   
                 VDLTEHLNQLNVKMQGIGNTVLSLQQAVFAFENKL 
               
               
                   
                   
               
               
                   
                 ELFTTDLETGRLLHFEKLSQFKDACTASEPIQNLD 
               
               
                   
                   
               
               
                   
                 LHQLAGCTSSLLQSFKARFGEFREHTRLFKFITHP 
               
               
                   
                   
               
               
                   
                 NECSLNTADLNYIPGVSVRDFEAEVADLKASDMWV 
               
               
                   
                   
               
               
                   
                 NKFKSLNEDLERITRQKAELASKHMWTEMKKLQPE 
               
               
                   
                   
               
               
                   
                 DQLILKTWNALPVTYHTLQRVSIAVLTMFGSTYAC 
               
               
                   
                   
               
               
                   
                 EQSFSHLKNIKSNLRSRLTDESLNACMKLNITKYQ 
               
               
                   
                   
               
               
                   
                 PDYKDISKSMQHQKSH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 119; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 145. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGGGG (SEQ ID NO: 5), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 31, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 5; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 31. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 5; and 2) a 3′TR comprising the nucleic acid sequence of CCCCTG (SEQ ID NO: 31). In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of CTGTATAG (SEQ ID NO: 196), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 196. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 119; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 145. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 57, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 57. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-3_XT) 
               
               
                   
                 SEQ ID NO: 57 
               
               
                   
                 MTSSQPAVKRKIDDEHRQFQEKWEMQYFFVEHRGI 
               
               
                   
                   
               
               
                   
                 PTCLICAEKVAVHKEYNLKRHYSTKHAEECAKYQG 
               
               
                   
                   
               
               
                   
                 DERAKRVASLKACLMRQQDFFKKATKENVASVQAS 
               
               
                   
                   
               
               
                   
                 YMVSEMIAKAGKPFTEGEFVKKCMLQVASIICPEK 
               
               
                   
                   
               
               
                   
                 KGQFSKISLSANTVAERISDMSSDIYHQLCEKAKC 
               
               
                   
                   
               
               
                   
                 FDAYSVALDESTDITGTAQLTIYVRGVDCNFELTE 
               
               
                   
                   
               
               
                   
                 ELLTIIPMHGQTTANEIFHHLCDAIENAGLPWKRF 
               
               
                   
                   
               
               
                   
                 VGIITDGAPSMTGRKNGLVALVKKKLEEEGIEEEA 
               
               
                   
                   
               
               
                   
                 IALHCIIHQQALCSKCLPCDNVMSVVVKCVNQIRS 
               
               
                   
                   
               
               
                   
                 RGLTHRRFRAFLEEMGSEYGDVLYFTEVRWLSRGN 
               
               
                   
                   
               
               
                   
                 VLKRFFELREEVKAFMEKNGKAVSELSDHKWLMDL 
               
               
                   
                   
               
               
                   
                 AFLVDITQRLNVLNKMLQGQGQLVSAAYDNVRAFS 
               
               
                   
                   
               
               
                   
                 TKLVLWKSQLSQTNLCHFPACKELVDAGIPFSGEK 
               
               
                   
                   
               
               
                   
                 YVDAIFKLEKEFDHRFADFKTHRATFQIFVDPFSF 
               
               
                   
                   
               
               
                   
                 DVQDAPPVLQMELIDLQCNSDIKAKFREMSGKADT 
               
               
                   
                   
               
               
                   
                 HVQFLRELPPSFPELSRMFKR 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 120; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 146. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 6, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 32, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 6; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 32. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 6; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 32. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 120; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 146. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 58, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 58. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-3_Xt) 
               
               
                   
                 SEQ ID NO: 6 
               
               
                   
                 CAGTGGAGGAAATAATTATTTGACCCCTCACTGAT 
               
               
                   
                   
               
               
                   
                 TTTGTAAGTTTGTCCAATGACAAAGAAATGAAAAG 
               
               
                   
                   
               
               
                   
                 TCTCAGAACAGTATCATTTCAATGGTAGGTTTATT 
               
               
                   
                   
               
               
                   
                 TTAACAGTGGCAGATAGCACATCAAAAGGAAAATC 
               
               
                   
                   
               
               
                   
                 GAAAAAATAACTTTAAATAAAAGATAGCAACTGAT 
               
               
                   
                   
               
               
                   
                 TTGCATTTCATTGAGTGAAATAAGTTTTTGAACCC 
               
               
                   
                   
               
               
                   
                 (3′TR of Tcl-3_Xt) 
               
               
                   
                 SEQ ID NO: 32 
               
               
                   
                 GGGTTCAAAAACTTATTTCACTCAATGAAATGCAA 
               
               
                   
                   
               
               
                   
                 ATCAGTTGCTATCTTTTATTTAAAGTTATTTTTTC 
               
               
                   
                   
               
               
                   
                 GATTTTCCTTTTGATGTGCTATCTGCCACTGTTAA 
               
               
                   
                   
               
               
                   
                 AATAAACCTACCATTGAAATGATACTGTTCTGAGA 
               
               
                   
                   
               
               
                   
                 CTTTTCATTTCTTTGTCATTGGACAAACTTACAAA 
               
               
                   
                   
               
               
                   
                 ATCAGTGAGGGGTCAAATAATTATTTCCTCCACTG 
               
               
                   
                   
               
               
                   
                 (transposase of Tcl-3_Xt) 
               
               
                   
                 SEQ ID NO: 58 
               
               
                   
                 MGKTKELSKDVRDKIVDLHKAGMGYKTISKKLGEK 
               
               
                   
                   
               
               
                   
                 VTTVGAIVRKWKEHKMTINRPRSGAPRKISPRGVS 
               
               
                   
                   
               
               
                   
                 MILRKVKKIIPRTTREELVNDLKLAGTTVTKKTIG 
               
               
                   
                   
               
               
                   
                 NTLIIRNGLKSCRARKVPLLKKAHVQARLKFANEH 
               
               
                   
                   
               
               
                   
                 LNDSVSDWEKVLWSDETKIELFGINSTRCVWRKKN 
               
               
                   
                   
               
               
                   
                 AAYDPQNTVPTVKHGGGNTLLWGCFSAKGTGQLIR 
               
               
                   
                   
               
               
                   
                 JNGKMDGAMYREILNDNLLPSARKLKMGRGWVFQH 
               
               
                   
                   
               
               
                   
                 DNDPKHTAKATKEWLKKKHIKVMEWPSQSPDLNPI 
               
               
                   
                   
               
               
                   
                 ENLWRELKLRVAQRQPRNLRDLEMICKEEWTNIPP 
               
               
                   
                   
               
               
                   
                 KMCANLVINYKKRLTSVLANKGFSTKY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 121; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 147. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGGGGTGGCGAACC (SEQ ID NO: 7), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of GGTTCGCCACCCCTG (SEQ ID NO: 33), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 7; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 33. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 7; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 33. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of GTCTATAC (SEQ ID NO: 194), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 194. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 121; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 147. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 59, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 59. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-5_DR) 
               
               
                   
                 SEQ ID NO: 59  
               
               
                   
                 MAESGKRTAKRKYEDERRTFLSEWEDLYFFVERNG 
               
               
                   
                   
               
               
                   
                 KPFCLICQTSLSHFKASNLERHFTSLHSAVAREFP 
               
               
                   
                   
               
               
                   
                 KGSELRKHKVKTLKGQAEKQTQLFRKFTKHSETVT 
               
               
                   
                   
               
               
                   
                 LASYQLAWNIARAKKPYLEGEFVKKCLSDAVAILC 
               
               
                   
                   
               
               
                   
                 PENENLKRSVKDLQLSRHTVEQRISDIDNSVETHL 
               
               
                   
                   
               
               
                   
                 LSDLQKCQYFSIALDESCDVQDKPQLAIFVRFVSE 
               
               
                   
                   
               
               
                   
                 DCTIREELLDIVPLKDRTRGIDLKETLMTVVEKAN 
               
               
                   
                   
               
               
                   
                 LQLSKLTAIVTDGAPAMLGSERGLVGLCKADDRFP 
               
               
                   
                   
               
               
                   
                 AFWTFHCIIHQEHLVSKKJLNLDHIMKPVLEIVNF 
               
               
                   
                   
               
               
                   
                 VRTHALNHRQFKNLIDELDEDLPSDLLFHCAVRWL 
               
               
                   
                   
               
               
                   
                 SRGHVLSRFFELLNPVKLFLAEKHKEYPELHDPQW 
               
               
                   
                   
               
               
                   
                 ISDLAFLVDVLHYLNGLNVDLQGKLKMLPDLVQSV 
               
               
                   
                   
               
               
                   
                 FAFVNKLKLFKTHLQKRDYTHFPTLLKASGQEAEV 
               
               
                   
                   
               
               
                   
                 VKGSTARYATLLENLEQSFEERFSNLRQKRQQITF 
               
               
                   
                   
               
               
                   
                 LINPFTAESGCLKAPLVEDEAASQLEMIELSEDDR 
               
               
                   
                   
               
               
                   
                 LKSVLREGTVEFWKIVPVERYPNVKQAALKLLSMF 
               
               
                   
                   
               
               
                   
                 GSTYVCESLFSTLKLVKSKHRSVLTDTHVKELLRV 
               
               
                   
                   
               
               
                   
                 ATTEYEPDLKKIVETKECQVSH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 122; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 148. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 8, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 34, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 8; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 34. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 8; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 34. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 122; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 148. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 60, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 60. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-3_FR) 
               
               
                   
                 SEQ ID NO: 8 
               
               
                   
                 CAGTGAGAGTAAAAAGTATTTGATCCCTTGCTGAT 
               
               
                   
                   
               
               
                   
                 TTTGTTGGTTTGTCCACTAATAAAGACATGATCAT 
               
               
                   
                   
               
               
                   
                 TCTATACTTTTAATGGTAGATGTATTCTAACATGG 
               
               
                   
                   
               
               
                   
                 AGAGACAGAATATCAAAAAGAAAATC 
               
               
                   
                   
               
               
                   
                 (3′TR of Tcl-3_FR) 
               
               
                   
                 SEQ ID NO: 34 
               
               
                   
                 GATTTTCTTTTTGATATTCTGTCTCTCCATGTTAG 
               
               
                   
                   
               
               
                   
                 AATACATCTACCATTAAAAGTATAGAATGATCATG 
               
               
                   
                   
               
               
                   
                 TCTTTATTAGTGGACAAACCAACAAAATCAGCAAG 
               
               
                   
                   
               
               
                   
                 GGATCAAATACTTTTTACTCTCACTG 
               
               
                   
                   
               
               
                   
                 (transposase ofTcl-3 FR) 
               
               
                   
                 SEQ ID NO: 60 
               
               
                   
                 MGKTKELSQDLRDRIVDLHKSGMGYKNISKLLGIK 
               
               
                   
                   
               
               
                   
                 VTTIGAIVRKFKKYNMTINRPRSGAPQKISPRGVA 
               
               
                   
                   
               
               
                   
                 MIMRTVRNRPATTQQELVNDLQAAGTKVTRKTIGN 
               
               
                   
                   
               
               
                   
                 TLRRNGLKSCSARKVPLLKKAHVEARLKYANDHLK 
               
               
                   
                   
               
               
                   
                 DAQSDWEKVLWSDETKIELFGLNSTRHVWRKKNAA 
               
               
                   
                   
               
               
                   
                 YDPRNTVPTVKHGGGNIMFWGCFSAKGTGLLHRIT 
               
               
                   
                   
               
               
                   
                 GKMDGAMYRAVLRDNLLPSARKLKMGRGWVFQHDN 
               
               
                   
                   
               
               
                   
                 DPKHTAKATKEWLKKNIIIKVMEWPSQSPDLNPIE 
               
               
                   
                   
               
               
                   
                 NLWRELKVRVAKRQPTNLNDLERICKEEWAKIPPD 
               
               
                   
                   
               
               
                   
                 MCANLVVNYNKRLTAVLANNGFATKY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 123; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 149. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 9, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 35, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 9; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 35. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 9; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 35. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 123; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 149. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 61, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 61. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-5_Xt) 
               
               
                   
                 SEQ ID NO: 9 
               
               
                   
                 CAAACCGGATTCCAAAAAAGTTGGGACACTAAACA 
               
               
                   
                   
               
               
                   
                 AATTGTGAATAAAAACTGAACGCAATGATGTGGAG 
               
               
                   
                   
               
               
                   
                 GTGCCAACTTCTAATATTTTATTCAGAATAGAACA 
               
               
                   
                   
               
               
                   
                 TAAATCACGGAACAAAAGTTTAAACTGAGAAAATG 
               
               
                   
                   
               
               
                   
                 TACCATTTTAAGGGAAAAATATGTTGATTCAGAAT 
               
               
                   
                   
               
               
                   
                 TTCATGGTGTCAACAAATCCCAAAAAAGTTGGGAC 
               
               
                   
                   
               
               
                   
                 AAG 
               
               
                   
                   
               
               
                   
                 (3′TR of Tcl-5_Xt) 
               
               
                   
                 SEQ ID NO: 35 
               
               
                   
                 CTTGTCCCAACTTTTTTGGGATTTGTTGACACCAT 
               
               
                   
                   
               
               
                   
                 GAAATTCTGAATCAACATATTTTTCCCTTAAAATG 
               
               
                   
                   
               
               
                   
                 GTACATTTTCTCAGTTTAAACTTTTGTTCCGTGAT 
               
               
                   
                   
               
               
                   
                 TTATGTTCTATTCTGAATAAAATATTAGAAGTTGG 
               
               
                   
                   
               
               
                   
                 CACCTCCACATCATTGCGTTCAGTTTTTATTCACA 
               
               
                   
                   
               
               
                   
                 ATTTGTTTAGTGTCCCAACTTTTTTGGAATCCGGG 
               
               
                   
                   
               
               
                   
                 TTG 
               
               
                   
                   
               
               
                   
                 (transposase of Tcl-5_Xt) 
               
               
                   
                 SEQ ID NO: 61 
               
               
                   
                 MIGYKKSFSEWQCLSEAKMGRGSPIPTMLRRKIVE 
               
               
                   
                   
               
               
                   
                 QYQKGVTQRKIAKILHLSSSTVHNIIRRFRESGTI 
               
               
                   
                   
               
               
                   
                 SVRKGQGRKTILDARDLRALKRHCTTNRNATVKEI 
               
               
                   
                   
               
               
                   
                 TEWAQEYFQKPLSVNTIHRAIRRCQLKLYSAKKKP 
               
               
                   
                   
               
               
                   
                 FLSKIHKLRRFHWARDHLKWSVAKWKTVLWSDESRF 
               
               
                   
                   
               
               
                   
                 EVLF 
               
               
                   
                   
               
               
                   
                 GNLGRHVIRTKEDKDNPSCYQRSVQKPASLMVWGC 
               
               
                   
                   
               
               
                   
                 MSACGMGSLHVWKGSINAEKYIQVLEQHMLPSRRH 
               
               
                   
                   
               
               
                   
                 LFQGRPCIFQQDNARPHSASITTSWLRRRRIRVLK 
               
               
                   
                   
               
               
                   
                 WPVCSPDLSPIENIWRIIKRKVRQRRPKTIEQLEA 
               
               
                   
                   
               
               
                   
                 CIRQEWESIPIPKLEKLVSSVPRRLLSVVRRRGDA 
               
               
                   
                   
               
               
                   
                 TQW 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 124; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 150. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 10, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 36, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 10; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 36. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 10; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 36. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 124; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 150. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 62, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 62. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-10_Xt) 
               
               
                   
                 SEQ ID NO: 10 
               
               
                   
                 CAGTGGCTTGCAAAAGTATTCGGCCCCCTTGAACT 
               
               
                   
                   
               
               
                   
                 TTTCCACATTTTGTCACATTACAGCCACAAACATG 
               
               
                   
                   
               
               
                   
                 AATCAATTTTATTGGAATTCCACGTGAAAGACCAA 
               
               
                   
                   
               
               
                   
                 TACAAAGTGGTGTACACGTGAGAAGTGGAACGAAA 
               
               
                   
                   
               
               
                   
                 ATCATACATGATTCCAAACATTTTTTACAAATAAA 
               
               
                   
                   
               
               
                   
                 TAACTGCAAAGTGGGGTGTGCGTAATTATTCAGCC 
               
               
                   
                   
               
               
                   
                 CCCT 
               
               
                   
                   
               
               
                   
                 (3′TR of Tcl-W_Xt) 
               
               
                   
                 SEQ ID NO: 36 
               
               
                   
                 AGGGGGCTGAATAATTACGCACACCCCACTTTGCA 
               
               
                   
                   
               
               
                   
                 GTTATTTATTTGTAAAAAATGTTTGGAATCATGTA 
               
               
                   
                   
               
               
                   
                 TGATTTTCGTTCCACTTCTCACGTGTACACCACTT 
               
               
                   
                   
               
               
                   
                 TGTATTGGTCTTTCACGTGGAATTCCAATAAAATT 
               
               
                   
                   
               
               
                   
                 GATTCATGTTTGTGGCTGTAATGTGACAAAATGTG 
               
               
                   
                   
               
               
                   
                 GAAAAGTTCAAGGGGGCCGAATACTTTTGCAAGCC 
               
               
                   
                   
               
               
                   
                 ACTG 
               
               
                   
                   
               
               
                   
                 (transposase of Tcl-10_Xt) 
               
               
                   
                 SEQ ID NO: 62 
               
               
                   
                 MKSKEHTRQVRDKVIEKFKAGLGYKKISKALNIPR 
               
               
                   
                   
               
               
                   
                 STVQAIIQKWKEYQTTVNLPRQGRPPKLTGKTRRA 
               
               
                   
                   
               
               
                   
                 LIRNAAKRPMVTLDKLQRSTAQVGESVHRTTISRA 
               
               
                   
                   
               
               
                   
                 LHKVGLYGRVARRKPLLTENHKKSRIQFATSIIVG 
               
               
                   
                   
               
               
                   
                 DTANMWKKVLWSDETKMELFGQNAKRYVWRKTNTA 
               
               
                   
                   
               
               
                   
                 HHSEHTIPTVKYGGGSIMLWGCFSSAGTGKLVRVD 
               
               
                   
                   
               
               
                   
                 GKMDGAKYRAILEENLLESAKDLRLGRRFTFQQDN 
               
               
                   
                   
               
               
                   
                 DPKHKARATMEWFKTKHIHVLEWPSQSPDLNPIEN 
               
               
                   
                   
               
               
                   
                 LWQDLKTAVHKRCPSNLTELELFCKEEWARISVSR 
               
               
                   
                   
               
               
                   
                 CAKLVETYPKRLAAVIAAKGGSTKY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 125; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 151. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of TACAGTGTCGGACAAATC (SEQ ID NO: 11), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of GATTTGTCCGACACTGTA (SEQ ID NO: 37), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 11; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 37. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 11; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 37. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 125; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 151. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 63, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 63. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Mariner2_AG) 
               
               
                   
                 SEQ ID NO: 63 
               
               
                   
                 MGRSAHSTQQQRLDIKRLSAAGYSQRKIAEILGRS 
               
               
                   
                   
               
               
                   
                 KTFVYNALHSTGTKIPTGRPRKTSARDDARMTRLC 
               
               
                   
                   
               
               
                   
                 KADPFKSARAIRDELQLSVSDRTVQRRLFENNLVG 
               
               
                   
                   
               
               
                   
                 RNPRKVPLLRRCHVQARLQFAREHYDWAGENLNKW 
               
               
                   
                   
               
               
                   
                 RNVLWSDESKVNLVGSDGKRFVRRPKNTAYRPQYT 
               
               
                   
                   
               
               
                   
                 LKTVKHGGGNIMVWACFSWYGVGPIFWIKDIMDQH 
               
               
                   
                   
               
               
                   
                 RYLNIIQTVMLPHAEWEMPLKWQFMHDNDPKHTAK 
               
               
                   
                   
               
               
                   
                 AVKKWFVDQKIDVMNWPAQSPDLNPIENLWKIVKA 
               
               
                   
                   
               
               
                   
                 KLPPAGSRTKEKLWKHIENA 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 126; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 152. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 12, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 38, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%4, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 12; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 38. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 12; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 38. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%4, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 126; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 152. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 64, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 64. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-1_Xt) 
               
               
                   
                 SEQ ID NO: 12  
               
               
                   
                 CAGTGGTGTGAAAAACTATTTGCCCCCTTCCTGAT 
               
               
                   
                   
               
               
                   
                 TTCTTATTCTTTTGCATGTTTGTCACAC 
               
               
                   
                   
               
               
                   
                 (3′TR of Tcl-1_Xt) 
               
               
                   
                 SEQ ID NO: 38 
               
               
                   
                 GTGTGACAAACATGCAAAAGAATAAGAAATCAGGA 
               
               
                   
                   
               
               
                   
                 AGGGGGCAAATAGTTTTTCACACCACTG 
               
               
                   
                   
               
               
                   
                 (transposase of Tcl-1_Xt) 
               
               
                   
                 SEQ ID NO: 64  
               
               
                   
                 MPRSKEIQEQMRTKVIEIYQSGKGYKAISKALGLQ 
               
               
                   
                   
               
               
                   
                 RTTVRAIIHKWQKIIGTVVNLPRSGRPTKITPRAQ 
               
               
                   
                   
               
               
                   
                 RQLIREATKDPRTTSKELQASLASIKVSVHDSTIR 
               
               
                   
                   
               
               
                   
                 KRLGKNGLHGRFPRRKPLLSKKNIKARLNFAKKHL 
               
               
                   
                   
               
               
                   
                 NDCQDFWENTLWTDETKVELFGRCVSRYIWRKSNT 
               
               
                   
                   
               
               
                   
                 AFQKKNIIPTVKYGGGSVMVWGCFAASGPGRLAVI 
               
               
                   
                   
               
               
                   
                 DGTMNSTVYQKILKENVRPSVRQLKLKRSWVLQQD 
               
               
                   
                   
               
               
                   
                 NDPKHTSKSTSEWLKKNKMKTLEWPSQSPDLNPIE 
               
               
                   
                   
               
               
                   
                 MLWHDLKKAVHARKPSNKAELQQFCKDEWAKIPPE 
               
               
                   
                   
               
               
                   
                 RCKRLVASYRKRLIAVIAAKGGPTSY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 127; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 153. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CACTGGTGGACAT (SEQ ID NO: 13), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of ATGTCCACCAGTG (SEQ ID NO: 39), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 13; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 39. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 13; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 39. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 127; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 153. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 65, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 65. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Tcl-1_AG) 
               
               
                   
                 SEQ ID NO: 65 
               
               
                   
                 MGRGKHCTPEERKHIQGLYRENVPIKTICKAFGRS 
               
               
                   
                   
               
               
                   
                 RTFVDNAIRSEATGKSTGRPRKTTADVDAQIVEMI 
               
               
                   
                   
               
               
                   
                 RADPFKTCTRIKQELGLQVSAKTVSRRLHAAGFCA 
               
               
                   
                   
               
               
                   
                 RRPRKVRKLLPHHVEARIRFAEEHLAASIFWWSKI 
               
               
                   
                   
               
               
                   
                 IFSDESRINLDGSDGIKYVWRFPNQAYHPKNTIKT 
               
               
                   
                   
               
               
                   
                 LSHGGGHVMVWGCFSWHGTGPLFRINGTLNSEGYR 
               
               
                   
                   
               
               
                   
                 KILSRKMLPYARQQFGDEEHYIFQHDNDSKHTSRT 
               
               
                   
                   
               
               
                   
                 VKCYLANQDVQVLPWPALSPDLNPIENLWSTLKRQ 
               
               
                   
                   
               
               
                   
                 LKNQPARSADDLWTRCKVMWERIPRSEC 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 128; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 154. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of ATATACAC (SEQ ID NO: 14), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of GTGTATAT (SEQ ID NO: 40), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 14; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 40. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 14; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 40. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of AT (SEQ ID NO: 198), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 198. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 128; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 1000) sequence identity to the nucleic acid sequence of SEQ ID NO: 154. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 66, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 66. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of TclDR3_Xt) 
               
               
                   
                 SEQ ID NO: 66  
               
               
                   
                 MGKKGDLSNFERGMVVGARRAGLSISQSAQLLGFS 
               
               
                   
                   
               
               
                   
                 RTTISRVYKEWCEKGKTSSMRQSCGRKCLVDARGQ 
               
               
                   
                   
               
               
                   
                 RRMGRLIQADRRATLTEITTRYNRGMQQSICEATT 
               
               
                   
                   
               
               
                   
                 RTTLRRMGYNSRRPHRVPLISTTNRKKRLQFAQAH 
               
               
                   
                   
               
               
                   
                 QNWTVEDWKNVAWSDESRFLLRHSNGRVRIWRKQN 
               
               
                   
                   
               
               
                   
                 ENMDPSCLVTTVQAGGGGVMVWGMFSWHTLGPLVP 
               
               
                   
                   
               
               
                   
                 IGHRLNATAYLSIVSDHVHPFMTTMYPSSDGYFQQ 
               
               
                   
                   
               
               
                   
                 DNAPCHKARIISNWFLEHDNEFTVLKWPPQSPDLN 
               
               
                   
                   
               
               
                   
                 PIEHLWDVVERELRALDVHPTNLHQLQDAILSIWA 
               
               
                   
                   
               
               
                   
                 NISKECFQHLVESMPRRIKAVLKAKGGQTPY  
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 129; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 155. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGGGGTCACCAAACT (SEQ ID NO: 15), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 41, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 15; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 41. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 15; and 2) a 3′TR comprising the nucleic acid sequence of AGTTTGGTGACCCCTG (SEQ ID NO: 41). In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of CTCTAGAC (SEQ ID NO: 199), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 199. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 129; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 155. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 67, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 67. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-3_PM) 
               
               
                   
                 SEQ ID NO: 67 
               
               
                   
                 MATKKRKVDAECRVFNEEWGVKYFFVETKDQKASC 
               
               
                   
                   
               
               
                   
                 VICSESVAVLKEYNLRRHYETKHLSTYSKFSGKLR 
               
               
                   
                   
               
               
                   
                 SEKYESMKRGLETQRNLFTRKFAENESVTRTSYKR 
               
               
                   
                   
               
               
                   
                 VHKMAERGKPFTDGNFIKECMMEAANDLCPERADL 
               
               
                   
                   
               
               
                   
                 FGSISLSASSVVRRTEELGENIVLQIREKARNLLW 
               
               
                   
                   
               
               
                   
                 YSLALDESTDLSSTSQLLVFIRGVNLDFQITEELA 
               
               
                   
                   
               
               
                   
                 SVCSMHGTTTGKDIFMEVQKTLQDYNLQWNQLRGV 
               
               
                   
                   
               
               
                   
                 TVDGGKRMAGVRKGLVGQLRTQLEDLQIPGALFIH 
               
               
                   
                   
               
               
                   
                 CIIHQQALCGKDLDISCVLKPVVSAVNFIRGHALN 
               
               
                   
                   
               
               
                   
                 HRQFQAFLEEMDSDFCDLPYIITAVRWLSCGKVLF 
               
               
                   
                   
               
               
                   
                 RFYKLRNEIDVFLTEKDRADPQLSDPTWLSKLSFL 
               
               
                   
                   
               
               
                   
                 VDITSIIMNELNLKLQGKDNLVCDLYRIIKGFRRK 
               
               
                   
                   
               
               
                   
                 LSLFEAQLEGENFSHFHCFKEFCATIAEDVNLDFP 
               
               
                   
                   
               
               
                   
                 KKIIRDLKKHFLKRFSDLDRIESDILLFQNPFDCN 
               
               
                   
                   
               
               
                   
                 LDDVPVELQLELIDLQANDLLKEKHREGKLVEFYR 
               
               
                   
                   
               
               
                   
                 CLPDVEFPKLKKFAAGMASVFGTTYVCEQTTSMAT 
               
               
                   
                   
               
               
                   
                 KKRKVDAECRVFNEEWGVKYFFVETKDQKASCVIC 
               
               
                   
                   
               
               
                   
                 SESVAVLKEYNLRRHYETKHLSKMKYVKSTHRTRL 
               
               
                   
                   
               
               
                   
                 TDEHLKAILLIGCSNSKPNIDDILKAKRQFHKSH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 130; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 156. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGGGGTCACCAAACT (SEQ ID NO: 16), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of AGTTTGGTGACCCCTG (SEQ ID NO: 42), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 16; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 42. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 16; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 42. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 130; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 1000) sequence identity to the nucleic acid sequence of SEQ ID NO: 156. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 68, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 68. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Tcl-1_PM) 
               
               
                   
                 SEQ ID No: 68 
               
               
                   
                 MATKKRKVDAECRVFNEEWGVKYFFVETRDQKASC 
               
               
                   
                   
               
               
                   
                 VCSEVAVLREYNLRRHYEIKHLSTYSKFSGKLRSE 
               
               
                   
                   
               
               
                   
                 KYESMKRGLETQRNLFTRKFAENESVTRTSYKIVH 
               
               
                   
                   
               
               
                   
                 KMAERGKPFTDGNFIKECMMEAANDLCPERADLFG 
               
               
                   
                   
               
               
                   
                 SISLSASSVVRRTEELGENIVLQIREKARNLLWYS 
               
               
                   
                   
               
               
                   
                 LALDESTDLSSTSQLLVFIRGVNLDFQITEELASV 
               
               
                   
                   
               
               
                   
                 CSMHGTTTGKDIFMEVQKTLQDYNLQWNQLRGVTV 
               
               
                   
                   
               
               
                   
                 DGGKNMAGVRKGLVGQIRTQLEDLQIPGALFIHCI 
               
               
                   
                   
               
               
                   
                 IHQQALCGKDLDISCVLKPVVSAVNFIRGHALNHR 
               
               
                   
                   
               
               
                   
                 QFQAFLEEMDSDFCDLPYHTAVRWLSCGKVLFRFY 
               
               
                   
                   
               
               
                   
                 KLRNEIDVFLTEKDRADPQLSDPTWLSKLSFLVDI 
               
               
                   
                   
               
               
                   
                 TSHMNELNLKLQGKDNLVCDLYRIIKGFRRKLSLF 
               
               
                   
                   
               
               
                   
                 EAQLEGENFSHFHCFKEFCATIAEDVNLDFPKKII 
               
               
                   
                   
               
               
                   
                 RDLKKHFLKRFSDLDRIESDILLFQNPFDCNLDDV 
               
               
                   
                   
               
               
                   
                 PVELQLELIDLQANDLLKEKHREGKLVEFYRCLPD 
               
               
                   
                   
               
               
                   
                 VEFPKLKKFAAGMASVFGTTYVCEQTFSKMKYVKS 
               
               
                   
                   
               
               
                   
                 THRTRLTDEHLKAILLIGCSNSKPNIDDILKAKRQ 
               
               
                   
                   
               
               
                   
                 FHKSH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 131; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 157. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGGC (SEQ ID NO: 17), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of GCCTG (SEQ ID NO: 43), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 17; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 43. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 17; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 43. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 131; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 157. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 69, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 69. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Mariner-4_AMi) 
               
               
                   
                 SEQ ID NO: 69 
               
               
                   
                 MSAKRKSMTSPGGSAKKQRKAIDLEMKMKIINDYE 
               
               
                   
                   
               
               
                   
                 AGKKVKAIARDIELAHSTISTILKDKDRVKEAV 
               
               
                   
                   
               
               
                   
                 QASTGFKAIITRQRKG 
               
               
                   
                   
               
               
                   
                 LIHEMEKLLAIWFDDQIQKRMPMSLLIIQAKARSI 
               
               
                   
                   
               
               
                   
                 FETLKAREGEESTETFTASRGWFQRFRRRFNVHNR 
               
               
                   
                   
               
               
                   
                 SISGEAASADVEAAEKFVDQFDEHEKGGYRPEQIF 
               
               
                   
                   
               
               
                   
                 NVDETGLFWKKMPERSYIHKEAKAMPGFKAFKDRV 
               
               
                   
                   
               
               
                   
                 TLLLGGNVAGFKLKPFLIHRSENPRALKQVSKHTL 
               
               
                   
                   
               
               
                   
                 PVYYRANSKAWMTQALFEDWFINCFIPSVKHYCLE 
               
               
                   
                   
               
               
                   
                 KGVPFKIILLLDNAPGHPQHILDDLHPDVKVVYLP 
               
               
                   
                   
               
               
                   
                 KNTTAILQPMDQGAIATFKVYYLRATFSKAVAATE 
               
               
                   
                   
               
               
                   
                 SDEVTLRDFWKSYNILHCIKNIESAWEGVTEKCMQ 
               
               
                   
                   
               
               
                   
                 GIWKKCLKVFVNNFEGFDKDEHVDVINKKIVELAN 
               
               
                   
                   
               
               
                   
                 VLNLDVEVKDIEELVEYVEGELTNEDLIELEAQQH 
               
               
                   
                   
               
               
                   
                 LEEEEEEEEERMEEVQKKFTVNGLAGVFSKVNAAI 
               
               
                   
                   
               
               
                   
                 LELEGMDPNVERFTKVERQMNELLRCYCEIYEEKK 
               
               
                   
                   
               
               
                   
                 KKKRKLQSRPH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 132; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 158. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGTGATGGCGAACCT (SEQ ID NO: 18), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of AGGTTCGCCATCACTG (SEQ ID NO: 44), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 18; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 44. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 18; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 44. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of GTCTAGAG (SEQ ID NO: 197), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 197. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 132; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100/o) sequence identity to the nucleic acid sequence of SEQ ID NO: 158. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 70, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 70. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Myotis_hAT1) 
               
               
                   
                 SEQ ID NO: 70  
               
               
                   
                 MENQKNKKARLNDKGSGSSSSRPFQDIWTEMYGIV 
               
               
                   
                   
               
               
                   
                 DRNNRSLCILCNETVVSRTWNVKRHFETNHCQLLK 
               
               
                   
                   
               
               
                   
                 KSIDERKEYISRQLHLYKSQSNSIIKFVRCSTNLT 
               
               
                   
                   
               
               
                   
                 SASYCIAHSIAQHGKALSDGEFIKETFLRCAPALF 
               
               
                   
                   
               
               
                   
                 HDMKNKDDIIKRISELPLSRNTIKDRIIDLNKNVQ 
               
               
                   
                   
               
               
                   
                 HQLKKDLNSCKYFSISLDETTDVTSNARLAIIARY 
               
               
                   
                   
               
               
                   
                 SDGLTMREELIKLESVPISTSGNEICKVVIKTFSD 
               
               
                   
                   
               
               
                   
                 LNIDISKIVSVTTDGAPNMVGKNVGFLKLFMEAIR 
               
               
                   
                   
               
               
                   
                 HPLVPFHCIIHQEVLCAKSGFSELNDLMSVVTKIV 
               
               
                   
                   
               
               
                   
                 NFIASRPLHKREFSALLQEVDSTYSGLLMFNNVRW 
               
               
                   
                   
               
               
                   
                 LSRGKVLERFVECFEEITVFIENKDLANFPQINDH 
               
               
                   
                   
               
               
                   
                 KWVSNLMFFTDLSVHIMNELNLKLQGFGKSIDVMF 
               
               
                   
                   
               
               
                   
                 GYIKSFESKLKIFKRDIETKTYKYFPRIKKYFEKA 
               
               
                   
                   
               
               
                   
                 TTTSVMESILMYQNIVNSLFEQFSERFDQFRSLEQ 
               
               
                   
                   
               
               
                   
                 TIKIIKYPDVVVYSTLELKDFQWMQIDDLEMQLAD 
               
               
                   
                   
               
               
                   
                 FQGSIWTHVFVDLRSKLENLERNRLNNEEECNYEQ 
               
               
                   
                   
               
               
                   
                 EILSAWNRVPDTFSDIKNLAMALLTIFSSTYFCET 
               
               
                   
                   
               
               
                   
                 LFSALNNIKTNKRNRLTDEVSGACLALKCTKYEPA 
               
               
                   
                   
               
               
                   
                 IFFLADKLQHQKSH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 133; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 159. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAG (SEQ ID NO: 19), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of CTG (SEQ ID NO: 45), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 19; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 45. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 19; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 45. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of GTCTAGAC (SEQ ID NO: 200), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 200. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 133; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 159. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 71, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 71. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of bAT-7_PM) 
               
               
                   
                 SEQ ID NO: 71 
               
               
                   
                 MSGSRKRKVDNECRVFNTEWTTKYFFTEVQSKAVC 
               
               
                   
                   
               
               
                   
                 LICRETVAVFKEYNISRHFATKHANYASKQSTQER 
               
               
                   
                   
               
               
                   
                 AATAQRLAANLQTQQHFFIIRQTAIQESTTKASFL 
               
               
                   
                   
               
               
                   
                 VAFEIAKASKPFSEGEFVKECMVQTADILCPEIKS 
               
               
                   
                   
               
               
                   
                 KFEKVSLSRRTVTRRVELIDENIASQLNKKSDSFE 
               
               
                   
                   
               
               
                   
                 LYSLALDESTDVKDTAQLLIFIRGIDDSFAITEEF 
               
               
                   
                   
               
               
                   
                 LTMESLKGTTRGEDIYNQVSAVIERMKIPWSKLVN 
               
               
                   
                   
               
               
                   
                 VTTDGSPNLTGKNVGLLKRIQNKVKEENPDQDLIF 
               
               
                   
                   
               
               
                   
                 LHCIIHQESLCKSVLQLNHVVNPAVKLVNFIRARG 
               
               
                   
                   
               
               
                   
                 LQHRQFITFLEETDADHQDLLYHSRVRWLSLGKVL 
               
               
                   
                   
               
               
                   
                 QRVWELKEDIIAFLELMGKSDEFPELSDKNWLSDF 
               
               
                   
                   
               
               
                   
                 AFAVDIFSHMNELNVKLQGKDQFVHDMYKHVKAFK 
               
               
                   
                   
               
               
                   
                 SKLTLFSRQIANKSFAHIFPTLAMQEEAPRNAKKY 
               
               
                   
                   
               
               
                   
                 SKSLEDLHGEFCRRFSDFENIEQSLQLVSCPLSQD 
               
               
                   
                   
               
               
                   
                 SETAPQELQLELIDLQSDSVLKEKFNSVKLNDFYA 
               
               
                   
                   
               
               
                   
                 SLNRATFPNLRRTAQKMLTLFGSTYVCEQTFSVMN 
               
               
                   
                   
               
               
                   
                 ANKARHRSKLTDQHLRSILRIATTKITPDLDALAK 
               
               
                   
                   
               
               
                   
                 MGDQQHCSH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 134; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 160. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 20, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 46, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 20; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 46. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 20; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 46. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 134; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 160. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 72, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 72. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-11_Xt) 
               
               
                   
                 SEQ ID NO: 20  
               
               
                   
                 CAGTGGATATAAAAAGTCTACACACCCCTGGTAAA 
               
               
                   
                   
               
               
                   
                 ATGTCAGGTTCCTGTGCTGTACAAAAATGAGACAA 
               
               
                   
                   
               
               
                   
                 AGATAAATCATTTCAGAACTTTTTCCACCTTTAAT 
               
               
                   
                   
               
               
                   
                 GTGACCTATAAACTGTACCACTCAATTGAAAAACA 
               
               
                   
                   
               
               
                   
                 AACTGAAATCTTTTAGG 
               
               
                   
                   
               
               
                   
                 (3′TR of TcMl_Xt) 
               
               
                   
                 SEQ ID NO: 46  
               
               
                   
                 CCTAAAAGATTTCAGTTTGTTTTTCAATTGAGTGG 
               
               
                   
                   
               
               
                   
                 TACAGTTTATAGGTCACATTAAAGGTGGAAAAAGT 
               
               
                   
                   
               
               
                   
                 TCTGAAATGATTTATCTTTGTCTCATTTTTGTACA 
               
               
                   
                   
               
               
                   
                 GCACAGGAACCTGACATTTTACCAGGGGTGTGTAG 
               
               
                   
                   
               
               
                   
                 ACTTTTTATATCCACTG 
               
               
                   
                   
               
               
                   
                 (transposase of Tcl-11 Xt) 
               
               
                   
                 SEQ ID NO: 72 
               
               
                   
                 MVHRELPKHQRDLIVKRYQSGEGYKRISKALDIPW 
               
               
                   
                   
               
               
                   
                 NTVKTVIIKWRKYGTTVTLPRTGRPSKIDEKTRRK 
               
               
                   
                   
               
               
                   
                 LVREATKRPTATLKELQEYLASTGCVVHVTTISRI 
               
               
                   
                   
               
               
                   
                 LHMSGLWGRVARRKPFLTKKNIQARLHFAKTHLKS 
               
               
                   
                   
               
               
                   
                 PKSMWEKVLWSDETKVELFGHNSKKYVWRKNNTAH 
               
               
                   
                   
               
               
                   
                 HQKNTIPTVKIIGGGSIMLWGCFSSAGTGALVKIE 
               
               
                   
                   
               
               
                   
                 GIMNSSKYQSILAQNLQASARKLNMRRNFIFQHDN 
               
               
                   
                   
               
               
                   
                 DPKHTSKSTKEWLHRKKIKVLEWPSQSPDLNPIEN 
               
               
                   
                   
               
               
                   
                 LWGDLKRAVHRRCPRNLTDLECFCKEEWANLAKSK 
               
               
                   
                   
               
               
                   
                 CAMLIDSYPKRLSAVIKSKGASTKY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 135; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 161. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 21, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 47, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 21; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 47. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 21; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 47. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 135; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 161. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 73, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 73. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-16 Xt) 
               
               
                   
                 SEQ ID NO: 21 
               
               
                   
                 CAGTCATGGCCAAAATTGTTGGCACCCCAGAAATT 
               
               
                   
                   
               
               
                   
                 TTTCCAGAAAATCAAGTATTTCTCACAGAAAAGTA 
               
               
                   
                   
               
               
                   
                 TTGCAGTAACACATGTTTTGCTATACACATGTTTA 
               
               
                   
                   
               
               
                   
                 TTCCCTTTGTGTGTATTGGAACAGAACAAAAAAGG 
               
               
                   
                   
               
               
                   
                 GAGGAAAAAAAGCAAATTGGACATAATGTCACACA 
               
               
                   
                   
               
               
                   
                 AAACTCCAAAAATGGGCTGGACAAAATTATTGGCA 
               
               
                   
                   
               
               
                   
                 CCCTT 
               
               
                   
                 (3′TR of Tcl-16_Xt) 
               
               
                   
                 SEQ ID NO: 47 
               
               
                   
                 AAGGGTGCCAATAATTTTGTCCAGCCCATTTTTGG 
               
               
                   
                   
               
               
                   
                 AGTTTTGTGTGACATTATGTCCAATTTGCTTTTTT 
               
               
                   
                   
               
               
                   
                 TCCTCCCTTTTTTGTTCTGTTCCAATACACACAAA 
               
               
                   
                   
               
               
                   
                 GGGAATAAACATGTGTATAGCAAAACATGTGTTAC 
               
               
                   
                   
               
               
                   
                 TGCAATACTTTTCTGTGAGAAATACTTGATTTTCT 
               
               
                   
                   
               
               
                   
                 GGAAAAATTTCTGGGGTGCCAACAATTTTGGCCAT 
               
               
                   
                   
               
               
                   
                 GACTG 
               
               
                   
                 (transposase of Tcl-16_Xt) 
               
               
                   
                 SEQ ID NO: 73 
               
               
                   
                 MDNRKRRRELSEDLRTKIVEKYQQSQGYKSISRDL 
               
               
                   
                   
               
               
                   
                 DLPLSTVRNIIKKFATHGTVANLPGRGRKRKIDER 
               
               
                   
                   
               
               
                   
                 LQRRIVRMVDKQPQTSSKEIQAVLQAQGASVSART 
               
               
                   
                   
               
               
                   
                 IRRHLNEMKRYGRRPRRTPILTQRHKKARLQFAKM 
               
               
                   
                   
               
               
                   
                 YISKPQSFWTNVLWTDETKIELFGKAHHSTVYRKR 
               
               
                   
                   
               
               
                   
                 NEAYKEKNTVPTVKYGGGSMMFWGCFAASGTGCLE 
               
               
                   
                   
               
               
                   
                 CVQGIMKSEDYQRILGRTVEPSVRKLGLRPRSWVF 
               
               
                   
                   
               
               
                   
                 QQDNDPKHTSKSTQKWMATKRWRVLKWPAMSPDLN 
               
               
                   
                   
               
               
                   
                 PIEHLWRDLKIAVGKRRPSNKRDLEQFAKEEWSKI 
               
               
                   
                   
               
               
                   
                 PGERCKKLIDGYRKRLISVIFSKGCATKY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 136; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 162. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 22, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 48, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 22; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 48. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 22; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 48. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 136; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 162. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 74, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 74. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of Tcl-4_Xt) 
               
               
                   
                 SEQ ID NO: 22 
               
               
                   
                 CAGTGAGGAACATAAGTATTTGAACACCCTGCGAT 
               
               
                   
                   
               
               
                   
                 TTTGCAAGTTCTCCCACTTAGAAATCATGGAGGGG 
               
               
                   
                   
               
               
                   
                 TCTGAAATTCACATTGTAGGTGCATTCCCACTGTG 
               
               
                   
                   
               
               
                   
                 AGAGACAGCATTTAAAAAAAAAATTCAGGAAATCA 
               
               
                   
                   
               
               
                   
                 CATTGTATGATTTTTAAAGAATGTATTTGTATTGC 
               
               
                   
                   
               
               
                   
                 ACTGCTGCACATAAGTATTTGAACACCTG 
               
               
                   
                   
               
               
                   
                 (3′TR of Tcl-4_Xt) 
               
               
                   
                 SEQ ID NO: 48 
               
               
                   
                 CAGGTGTTCAAATACTTATGTGCAGCAGTGCAATA 
               
               
                   
                   
               
               
                   
                 CAAATACATTCTTTAAAAATCATACAATGTGATTT 
               
               
                   
                   
               
               
                   
                 CCTGAATTTTTTTTTTAAATGCTGTCTCTCACAGT 
               
               
                   
                   
               
               
                   
                 GGGAATGCACCTACAATGTGAATTTCAGACCCCTC 
               
               
                   
                   
               
               
                   
                 CATGATTTCTAAGTGGGAGAACTTGCAAAATCGCA 
               
               
                   
                   
               
               
                   
                 GGGTGTTCAAATACTTATGTTCCTCACTG 
               
               
                   
                   
               
               
                   
                 (transposase of Tcl-4_Xt) 
               
               
                   
                 SEQ ID NO: 74 
               
               
                   
                 MGKTKELSKDTRDKIVDLHKAGKGYGAIAKQLGEN 
               
               
                   
                   
               
               
                   
                 RSTVGAIVRKWKRLKTTVSLPRTGAPCKISPRGVS 
               
               
                   
                   
               
               
                   
                 LMIRKVRNQPRTTREELVNDMKRAGTTVSKVTVGR 
               
               
                   
                   
               
               
                   
                 TLRRHGFKSCIARKVPLLKSSHVQARLKFANDHLD 
               
               
                   
                   
               
               
                   
                 DPEEAWEKVMWSDETKVELFGLNFTRRVWRKNKDE 
               
               
                   
                   
               
               
                   
                 LHPKNTIPTVKHGGGNIMLWGCFSAKGTGRLHCIK 
               
               
                   
                   
               
               
                   
                 ERMNGAMYCEIISNNLLPSVRALKMGRGWVFQHDN 
               
               
                   
                   
               
               
                   
                 DPKHTARITKEWLRKRHIKVLEWPSQSPDLNPIEN 
               
               
                   
                   
               
               
                   
                 LWRELKLRVAQRQPRNLTDLEEICVEEWAKIPVAV 
               
               
                   
                   
               
               
                   
                 CANLVKNYRKRLTSVIANKGFCTKY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 137; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 163. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CACTGCTCAAAAAAATAAAGGGAACAC (SEQ ID NO: 23), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of GTGTTCCCTTTATTTTTTTGAGCAGTG (SEQ ID NO: 49), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 23; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 49. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 23; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 49. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 137; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 163. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 75, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 75. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Tcl-15_Xt) 
               
               
                   
                 SEQ ID NO: 75 
               
               
                   
                 MRRSLQPTQMAQVVQLIQDGTSMRAVARRFAVSVS 
               
               
                   
                   
               
               
                   
                 VVSRAWRRYQETGQYIRRRGGGRRRATTQQQDRYL 
               
               
                   
                   
               
               
                   
                 RLCARRNRRSTARALQNDLQRATNVHVSAQTVRNR 
               
               
                   
                   
               
               
                   
                 LHEGGMRARRPQVGVVLTAQHRAGRLAFAREHQDW 
               
               
                   
                   
               
               
                   
                 QIRHWRPVLFTDESRFTLSTCDRRDRVWRRRGERS 
               
               
                   
                   
               
               
                   
                 AACNILQHDRFGSGSVMVWGGISLGGRTALHVLAR 
               
               
                   
                   
               
               
                   
                 GSLTAIRYRDEILRPLVRPYAGAVGPGFLLMQDNA 
               
               
                   
                   
               
               
                   
                 RPHVAGVCQQFLQDEGIDAMDWPARSPDLNPIEHI 
               
               
                   
                   
               
               
                   
                 WDIMSRSIHQRHVAPQTVQELVDALVQVWEEIPQE 
               
               
                   
                   
               
               
                   
                 TIRHLIRSMPRRCREVIQARGGHTHY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 138; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 164. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CACTGCTCAAAAAAATTAGAGGAACACTT (SEQ ID NO: 24), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of AAGTGTTCCTCTAATTTTTTTGAGCAGTG (SEQ ID NO: 50), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 24; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 50. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 24; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 50. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 138; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 164. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 76, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 76. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of TC1_FR2) 
               
               
                   
                 SEQ ID NO: 76 
               
               
                   
                 MVRYLDATEVAQVVQLLQDGTSIRAVARRFAVSPS 
               
               
                   
                   
               
               
                   
                 TVSRAWRRFQETGSYSRRAGQGRRRSLNPQQDRYL 
               
               
                   
                   
               
               
                   
                 LLCARRNRMSTARALQNDLQQATGVNVSDQTIRNR 
               
               
                   
                   
               
               
                   
                 LHEGGLRARRPVVGPVLTARIIRRARLAFAIDHQN 
               
               
                   
                   
               
               
                   
                 WQLRHWRPVLFTDESRFNLSTCDRRERVWRCRGER 
               
               
                   
                   
               
               
                   
                 YAACNIIQHDRFGGGSVMVWGGISLEGRTDLYRLD 
               
               
                   
                   
               
               
                   
                 NGTLTAIRYRDEILGPIVRTYAGAVGPGFLLVHDN 
               
               
                   
                   
               
               
                   
                 ARPHVARVCRQFLEDEGIDTIDWPPRSPDLNPIEH 
               
               
                   
                   
               
               
                   
                 LWDIMFRSIRRRQVDPQTVQELSDALVQIWEEIPQ 
               
               
                   
                   
               
               
                   
                 DTIRRLIRSMPRRCQACVQARGGHTNY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 139; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 165. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of TAG (SEQ ID NO: 25), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of CTA (SEQ ID NO: 51), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 901% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 25; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 51. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 25; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 51. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of ATCATCAT (SEQ ID NO: 201), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 201. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 139; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 165. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 77, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 77. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-9_XT) 
               
               
                   
                 SEQ ID NO: 77 
               
               
                   
                 MPGKGRPPRRGTRGRAAMISCGPRKLPSFPKALTL 
               
               
                   
                   
               
               
                   
                 NSQNAEEVVDWLTQHTPSSTVSNFATTSSSSSAMG 
               
               
                   
                   
               
               
                   
                 TPRTTSSTTAAPSSLESEELFTHEFVELSDAQPLL 
               
               
                   
                   
               
               
                   
                 PEEDEGDEDVTPDIIIQAGNTTEMDIRCDEVPAAA 
               
               
                   
                   
               
               
                   
                 VFCELSEEIDASEENDDEEIDILWVPTRREQEEDS 
               
               
                   
                   
               
               
                   
                 SDGETESQRGRRRIRLRRSRDSSQGTVGQQIIESA 
               
               
                   
                   
               
               
                   
                 PVVSQPTIIPPTSTVTARKPTSKGSAVWHFFNVCA 
               
               
                   
                   
               
               
                   
                 SDKSAVICNECSQKLSLGKPNTIILGTTAMRRIIM 
               
               
                   
                   
               
               
                   
                 NAKIIKALWEQIILKGSSQTLSIIPPSAPASYCST 
               
               
                   
                   
               
               
                   
                 SAVLDPSQPPSTPPSTLTTSSCSSAPSQVSVRAMF 
               
               
                   
                   
               
               
                   
                 ERKKPMPPSHPLARRLTAGESALLARQLLPYQLVD 
               
               
                   
                   
               
               
                   
                 SEAFHQFVAIGTPQWKVPSRNFFSQKGIPHLYQHV 
               
               
                   
                   
               
               
                   
                 QSQVTASLSFSVGAKVHMTTDTWSSKHGQGRYVTY 
               
               
                   
                   
               
               
                   
                 TAHWVNLVMAGKQGMRGSTTVELVSPPRVACGSAT 
               
               
                   
                   
               
               
                   
                 TSTPPLLSNSSSKSSSNSSSNLASSSASSSSAAVS 
               
               
                   
                   
               
               
                   
                 SSTPVHPQLHIGYSTCQVRRCHAVLGMTGLESRNH 
               
               
                   
                   
               
               
                   
                 TGSALLSSLHSQADRWLTPHQLKIGKVVCDNGSNL 
               
               
                   
                   
               
               
                   
                 LAALRLGNLTHVPCMAHVLNLIVQRFVSKYPGLQD 
               
               
                   
                   
               
               
                   
                 ILRQSRKVSGHFRRSYTAMARLADIQRRIINLPVR 
               
               
                   
                   
               
               
                   
                 RLICDSQTRWNSTLMMFERLLQQQRAVNTSTEYLF 
               
               
                   
                   
               
               
                   
                 ELGGRTGSAELGIFFPRYWVLMRDACRLMRPFDEV 
               
               
                   
                   
               
               
                   
                 TNMVSRTEGTISDLIPFAFFLERAVRRVTDDAVDQ 
               
               
                   
                   
               
               
                   
                 RDQEHDFWAESPERSQAPAATQGEVSEVESEEEGG 
               
               
                   
                   
               
               
                   
                 FVEAEDQQEQASRGACGHLLWSPGLVRGWGEETVV 
               
               
                   
                   
               
               
                   
                 DEDVVLDNEEGEMDTSASNLVRMGSFMLSCLLKDP 
               
               
                   
                   
               
               
                   
                 RIKRLKEKDLYWYATLLDPRYKHKVAEMLPTYHBC 
               
               
                   
                   
               
               
                   
                 SERMLHFQTSLQNMLYNAFKGDVTPHSRGRGAGNP 
               
               
                   
                   
               
               
                   
                 PTSTPARTMHFGHSVTSDMQSFFSPRQRQDPSGST 
               
               
                   
                   
               
               
                   
                 LRERLDRQVADYLALTADIDTLRSDEPLDYWVRRL 
               
               
                   
                   
               
               
                   
                 DLWPELSQFAINLLSCPASSVLSERTFSAAGGIVT 
               
               
                   
                   
               
               
                   
                 EKRTRLGIIKSVDYLTFIKMNEAWISEGYCTPEDL 
               
               
                   
                   
               
               
                   
                 FPEDLF 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 140; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 168. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CCGTATTTTCCGCACTATAAGGCGCACC (SEQ ID NO: 26), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of GGTGCGCCTTATAGTGCGGAAAATACGG (SEQ ID NO: 52), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%4, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 26; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 52. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 26; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 52. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%4, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 140; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 168. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 78, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 78. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Mariner-5_XT) 
               
               
                   
                 SEQ ID NO: 78 
               
               
                   
                 MAPKKRHAYEAQFKLQAASYAVVNGNRAAAKEFNI 
               
               
                   
                   
               
               
                   
                 NESMVRKWRKQENELRQVKKTKQSFRGNKARWPQL 
               
               
                   
                   
               
               
                   
                 EDQLEQWVIEQRTAGRSVSTVTIRLKATTIAQDLE 
               
               
                   
                   
               
               
                   
                 IEHFQGGPSWCFRFMKRRHLSIRARTTVAQQLPAD 
               
               
                   
                   
               
               
                   
                 YKEKMAIFRTYCSNKITDKKIQPNHITNMDEVPLT 
               
               
                   
                   
               
               
                   
                 FDIPVNHTVEIKGTSTVSIRTTGHEKSAFTVVLSC 
               
               
                   
                   
               
               
                   
                 HGNGQKLPPMVIFKRKTLPKEKFPAGVIVKANQKG 
               
               
                   
                   
               
               
                   
                 WMDEEKMREWLREVYVKRPDGFFHTSPSLLICDSM 
               
               
                   
                   
               
               
                   
                 RAHLTATVKKQVKQMNSELAIIPGGLTKELQPLDV 
               
               
                   
                   
               
               
                   
                 GVNRAFKVKLRTAWERWMTDGEHTFTKTGKQRRAS 
               
               
                   
                   
               
               
                   
                 YATICEWIVDAWAKVSAITVVRAFAKTGIIAEQPP 
               
               
                   
                   
               
               
                   
                 GNDTGNETDSDNDEREPGMFDGEIAQLFNSDTEDE 
               
               
                   
                   
               
               
                   
                 DFDGFVGED 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 167; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 179. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CCGTATTTTCTC (SEQ ID NO: 79), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of GAGAAAATACGG (SEQ ID NO: 91), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 79; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 91. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 79; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 91. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 167; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 179. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 103, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, %%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 103. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Mariner-6_AMi) 
               
               
                   
                 SEQ ID NO: 103 
               
               
                   
                 MPKRKSYTFDFKLSVIDYAKKHGNRAAGREFSVDE 
               
               
                   
                   
               
               
                   
                 KSVREWRKEEDILKTLNPRKRARRGKHCKWPTLEA 
               
               
                   
                   
               
               
                   
                 NLKEWVVAQRESNKVVSTIAIRQKARVMANEMKIT 
               
               
                   
                   
               
               
                   
                 DFGGGCNWVHKFMHRNNLAVRSRTTVGQKLPDDWE 
               
               
                   
                   
               
               
                   
                 EKLTKFRDFVRKECRVHNLCPADIINMDEVPISFD 
               
               
                   
                   
               
               
                   
                 VPATRTVDQRGKKTIAISTTGHERTNFTVVLACTA 
               
               
                   
                   
               
               
                   
                 SGVKLKPMVIFKRVTMPREKIPNGVAVICNKKGWM 
               
               
                   
                   
               
               
                   
                 NEDIMKEWTERCFRTRQGGFFAPKSLLIFDAMAAH 
               
               
                   
                   
               
               
                   
                 KHSSVQKQINKAGAHIAVIPGGLTCKLQPLDISVN 
               
               
                   
                   
               
               
                   
                 HSFKCFMRKEWEKWMSQGVHSFTPSGKQRRATYTE 
               
               
                   
                   
               
               
                   
                 VCNWIVAAWRAVKPSSIINGFFKAGILDGASGEES 
               
               
                   
                   
               
               
                   
                 DYTDSEDYADELDDAAFVDAYFGESSYDTDFEGFP 
               
               
                   
                   
               
               
                   
                 ASEKEEAPNWV 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 168; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 180. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CCCTTT (SEQ ID NO: 80), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of AAAGGG (SEQ ID NO: 92), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 80; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 92. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 80; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 92. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of TTAA (SEQ ID NO: 202), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 202. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 168; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 180. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 104, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 104. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase ofpiggyBac-2_XT) 
               
               
                   
                 SEQ ID NO: 104 
               
               
                   
                 MAKRFYSAEEAAAHCMASSSEEFSGSDSEYVPPAS 
               
               
                   
                   
               
               
                   
                 ESDSSTEESWCSSSTVSALEEPMEVDEDVDDLEDQ 
               
               
                   
                   
               
               
                   
                 EAGDRADAAAGGEPAWGPPCNFPPEIPPFTTVPGV 
               
               
                   
                   
               
               
                   
                 KVDTSNFEPINFFQLFMTEAILQDMVLYTNVYAEQ 
               
               
                   
                   
               
               
                   
                 YLTQNPLPRYARAHAWHPTDIAEMKRFVGLTLAMG 
               
               
                   
                   
               
               
                   
                 LIKANSLESYWDTTTVLSIPVFSATMSRNRYQLLL 
               
               
                   
                   
               
               
                   
                 RFLHFNNNATAVPPDQPGHDRLHKLRPLIDSLSER 
               
               
                   
                   
               
               
                   
                 FAAVYTPCQNICIDESLLLFKGRLQFRQYIPSKRA 
               
               
                   
                   
               
               
                   
                 RYGIKFYKLCESSSGYTSYFLIYEGKDSKLDPPGC 
               
               
                   
                   
               
               
                   
                 PPDLTVSGKIVWELISPLLGQGFHLYVDNFYSSIP 
               
               
                   
                   
               
               
                   
                 LFTALYCLDTPACGTINRNRKGLPRALLDKKLNRG 
               
               
                   
                   
               
               
                   
                 ETYALRKNELLAIKFFDKKNVFMLTSIHDESVIRE 
               
               
                   
                   
               
               
                   
                 QRVGRPPKNKPLCSKEYSKYMGGVDRTDQLQHYYN 
               
               
                   
                   
               
               
                   
                 ATRKTRAWYKKVGIYLIQMALRNSYIVYKAAVPGP 
               
               
                   
                   
               
               
                   
                 KLSYYKYQLQILPALLFGGVEEQTVPEMPPSDNVA 
               
               
                   
                   
               
               
                   
                 RLIGKIIFIDTLPPTPGKQRPQKGCKVCRKRGIRR 
               
               
                   
                   
               
               
                   
                 DTRYYCPKCPRNPGLCFKPCFEIYHTQLHY 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 169; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 181. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CCTTCATACG TTCCCATG (SEQ ID NO: 81), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of CATGAGAACG GATGAGGG (SEQ ID NO: 93), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 81; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 93. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 81; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 93. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 202, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 202. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 169; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 181. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 105, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 105. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of piggyBac-1_AMi) 
               
               
                   
                 SEQ ID NO: 105 
               
               
                   
                 MRRQRIFSAEACLAQVTGDDSDTSTDEILSQGSED 
               
               
                   
                   
               
               
                   
                 ADFVPDMGDCSEQEDHASTEASSSSDEEELVPQAE 
               
               
                   
                   
               
               
                   
                 VMPTLPVGKGRGPARGRRGRPRRATAGRGAEPLPP 
               
               
                   
                   
               
               
                   
                 PPENIADDDTFRGRNGRVWGKSPQLIHHRRTAKDI 
               
               
                   
                   
               
               
                   
                 VHESAGPTRESNKDTVVETFHILFLSQDILTVIIR 
               
               
                   
                   
               
               
                   
                 ETNREAERRFREWNDSHPENRKEWKKLDETELKAY 
               
               
                   
                   
               
               
                   
                 IGLLLLSGLYHGGHEALEELWEQRTGRPCFRATMS 
               
               
                   
                   
               
               
                   
                 LKRFRQITNYLRFDNKLTREERRAVDKLAAFRDIW 
               
               
                   
                   
               
               
                   
                 TQFVEQLRKWYIPGIHLTVDEQLIAFRGRCPFRQY 
               
               
                   
                   
               
               
                   
                 MPSKPAKYGLKIWWCCDAATSFPLSGDIYLGKQPE 
               
               
                   
                   
               
               
                   
                 APRQGNLGTQVIEKLVHPWYKTGRNIVGDNFFTST 
               
               
                   
                   
               
               
                   
                 DLAERLLQQDLTYVGTIRKNKADIPNEMQANRRRE 
               
               
                   
                   
               
               
                   
                 TFSSIFAFDGELTLVSYVPKKSKVVLLLSSMHHDL 
               
               
                   
                   
               
               
                   
                 SVGGEQRKPDIIHDYNKNKGGVDNLDHLVRMTTCR 
               
               
                   
                   
               
               
                   
                 RKINRWPMTLFFNMLDVAAIASLIIWLGHNANWKQ 
               
               
                   
                   
               
               
                   
                 RSHLRRRRFFLQELGEQLIDGQIKRRMMDTRNLSK 
               
               
                   
                   
               
               
                   
                 SVKSGMTALNYKLPQRTPVEPSASMSGRCAFCPRT 
               
               
                   
                   
               
               
                   
                 DDRKTRKRCEDCKRFACVH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 170; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 182. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGTAGAACC CCG (SEQ ID NO: 82), a variant thereof, or a fragment thereof, and 2) a 3′TR comprising the nucleic acid sequence of CGGGGTTCTACTG (SEQ ID NO: 94), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 82; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 94. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 82; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 94. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 170; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 182. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 106, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 106. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Mariner-3_Crp) 
               
               
                   
                 SEQ ID NO: 106 
               
               
                   
                 MRRPSQMELRAAWVPKQDSGFSLSVATVFKMSASN 
               
               
                   
                   
               
               
                   
                 TDSTSASASTRKRKAITLEEKLEVVKRYERGEKTK 
               
               
                   
                   
               
               
                   
                 EIRCATGLSESTLRTIRDNAEKIKESCKSATRLST 
               
               
                   
                   
               
               
                   
                 ARVSLTRSAIMEKMERMLTTWIEHQNQEHVPLSTQ 
               
               
                   
                   
               
               
                   
                 VIQAKARSIYEVLKRDDPEAKPFNASAGWFDRFKA 
               
               
                   
                   
               
               
                   
                 RHGFHNLKLTGEAAAADKSAADKFPSILQAAIEEG 
               
               
                   
                   
               
               
                   
                 SYDPRQVFNLDETGLFWKRMPSRTFISVEERTAPG 
               
               
                   
                   
               
               
                   
                 FKAAKDRCTLLLGANAAGDFKLKPLMVYHAENPRA 
               
               
                   
                   
               
               
                   
                 LKGYAKGHLPVYWRANRKGWVTGSLFEDYFSSLLH 
               
               
                   
                   
               
               
                   
                 LELRAYCDRQNLPFKILLLLDNAPGHPPSLGDLSE 
               
               
                   
                   
               
               
                   
                 NIKVLFLPPNTTSLIQPMDQGVIRAFKSYYLRRTF 
               
               
                   
                   
               
               
                   
                 KKLINETESKEKANVKEFWKAFNIKMAIDIIGDAW 
               
               
                   
                   
               
               
                   
                 NDVTQQCLNGVWKNIWPAVVNDFRGFAADEFISDA 
               
               
                   
                   
               
               
                   
                 RHEIVEMAKSVGFEQVDEENVAELLDSHREELSNE 
               
               
                   
                   
               
               
                   
                 DLLELDRERHEEEEPMEENERPSPRVLTMKGMADA 
               
               
                   
                   
               
               
                   
                 FKLLDGYLAFFDENDPNRERSAKVARLMKEANACY 
               
               
                   
                   
               
               
                   
                 TELYREKRRCSVQSSLDKFFRKRDSTISTQSTDSQ 
               
               
                   
                   
               
               
                   
                 PSTSGGEPATSGGEISPPSFDVFDLDEDSAPH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 171; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 183. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGTGGTTCT TAACCT (SEQ ID NO: 83), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of AGGTTAAGAA CCACTG (SEQ ID NO: 95), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 83; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 95. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 83; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 95. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of CTCTAGAG (SEQ ID NO: 203), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 203. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 171; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 183. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 107, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 107. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-1_PM) 
               
               
                   
                 SEQ ID NO: 107 
               
               
                   
                 MSGKRRKWSDEYVQHGFSCITERDGSERPICMICN 
               
               
                   
                   
               
               
                   
                 AKLSNSSLAPAKLKEHFLKLHGDGEYKNTTLAEFR 
               
               
                   
                   
               
               
                   
                 VKRARFDERATLPALGFVPVNKPILTASYEVAYLI 
               
               
                   
                   
               
               
                   
                 AKQGKPHTIGETLVKPAALKMANLILGTAAEGKLS 
               
               
                   
                   
               
               
                   
                 QIPLSNDTVSDRIEDMSKDILAQVVADLISSPAKF 
               
               
                   
                   
               
               
                   
                 SLQLDETTDVSDLSQLAVFVRYVKDDMIKEEFLFC 
               
               
                   
                   
               
               
                   
                 KPLTTTTKAADVKKLVDDFFRDNNLSWDMVSAVCT 
               
               
                   
                   
               
               
                   
                 DGAPVMLGRKSGFGALVKADAPHIIVTHCILHRHA 
               
               
                   
                   
               
               
                   
                 LATKTLPPKMAEVLKTVVECVNYVRKSALRHRIFS 
               
               
                   
                   
               
               
                   
                 ELCKEMGSEFEVLLLHSNIRWLSRGQVLNRVFAMR 
               
               
                   
                   
               
               
                   
                 VELALFLQEHQHCHADCFKNPEFILTLAYMADTFA 
               
               
                   
                   
               
               
                   
                 ALNHLNQQMQGGGVNIMEAEEHLKAFQKKLPLWKR 
               
               
                   
                   
               
               
                   
                 RTENNNFANFPLLDDCASKIEDVSGIGDISVSTEL 
               
               
                   
                   
               
               
                   
                 KQTIATHLDELAKSLDGYFPTRESYPAWVRQPFTF 
               
               
                   
                   
               
               
                   
                 AVETADVNDEFLDEITEIQQSQVQQQLFRTTTLST 
               
               
                   
                   
               
               
                   
                 FWCRQMNKYPVIAKKALDFFIPFVTTYLCEQSFSR 
               
               
                   
                   
               
               
                   
                 MLDIKTKKRNRLCCENDMRVALAKVKPRISELISQ 
               
               
                   
                   
               
               
                   
                 RQQQKSH 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 172; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 184. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of TAGGGCTGTG CGAAA (SEQ ID NO: 84), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of TTTCGCACAG CCCTA (SEQ ID NO: 96), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 84; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 96. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 84; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 96. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 196, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 1%. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 172; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 184. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 108, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 108. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-17_Croc) 
               
               
                   
                 SEQ ID NO: 108 
               
               
                   
                 MTVSSTPISLPMLSLPVPEEELELDLDLSAIAREI 
               
               
                   
                   
               
               
                   
                 LGKEGESSEFVLHTPQVSPRARSPSPEGTSEEAEV 
               
               
                   
                   
               
               
                   
                 EVAEASGSAESAPPVAVPLPAEEGEKAASAHAPAP 
               
               
                   
                   
               
               
                   
                 QKRAGSVVWDHFELADDPTYAICLHCRAKTSRGKG 
               
               
                   
                   
               
               
                   
                 TKHMSTTGMLMHLRRHHPLALAPPQPGTSGSVPKG 
               
               
                   
                   
               
               
                   
                 KSPSRSKAPVPPKQRQATLEQWGKGGQKGGRVARA 
               
               
                   
                   
               
               
                   
                 SEITQSIGEMLAVDGQPFSLVERPGFRRLMALVAP 
               
               
                   
                   
               
               
                   
                 SYQVPARTTFSRTVVPSLYEACREYLREELRKAGP 
               
               
                   
                   
               
               
                   
                 QVALHFTSDIWSSRGGDHAYLSLTGHWCDQSGRRW 
               
               
                   
                   
               
               
                   
                 ALLQAEVMDESHTATETMGAMNRLVQGWLVGQGEL 
               
               
                   
                   
               
               
                   
                 TRGFMVTDNGANMVKAVRDANFVGIRCVAHKFHLI 
               
               
                   
                   
               
               
                   
                 VRDALEGDRAAGDGASTTSKLISKCRKVAGYFHRS 
               
               
                   
                   
               
               
                   
                 IKGGKMLRDKQAELNIPQHKIMQDVETRWNSTYLM 
               
               
                   
                   
               
               
                   
                 LERLVEQQKAIHEMALLGEIGISGPLNRAEWDTIS 
               
               
                   
                   
               
               
                   
                 QILVVLKPFLEATETLSAGDALLSQVIPVVRELQS 
               
               
                   
                   
               
               
                   
                 QMESFQGINVPGWGKPLSPDVQALVRRLKEGIRKR 
               
               
                   
                   
               
               
                   
                 LDPLRSSTVHVLAATCDPRVKGSVCGSQSLNQWTE 
               
               
                   
                   
               
               
                   
                 VLVKRVREAERRRRGDVEEGDPLSRASTPSTSQSP 
               
               
                   
                   
               
               
                   
                 PPPQALPMWAKGMASMVGSRGTRPHHQAGSAQALV 
               
               
                   
                   
               
               
                   
                 AAYLAEDVEPLLCDPLAYWASRSQMWPDLATVARE 
               
               
                   
                   
               
               
                   
                 HLSCPPTSVPSERVFSIAGDVVTPHRTSLDPGLVE 
               
               
                   
                   
               
               
                   
                 QLVFLKVNLPLIGFPKIQVQTE  
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 173; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 185. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGGTTGAG (SEQ ID NO: 85), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of CTCAACCTG (SEQ ID NO: 97), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 85; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 97. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 85; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 97. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 173; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 185. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 109, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 109. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Tigger4) 
               
               
                   
                 SEQ ID NO: 109 
               
               
                   
                 MSKRPADTPMGNSDKKKRKHLCLSIAQKVKLLEKL 
               
               
                   
                   
               
               
                   
                 DSGVSVKRLTEEYGVGMTTIYDLKKQKDKLLKFYA 
               
               
                   
                   
               
               
                   
                 ESDEQKLMKNRKTLHKAKNEDLDRVLKEWIRQRRS 
               
               
                   
                   
               
               
                   
                 EHMPLNGMLIMKQAKIYHDELKIEGNCEYSTGWLQ 
               
               
                   
                   
               
               
                   
                 KFKKRHGIKFLKICGDKASADHEAAEKFIDEFAKV 
               
               
                   
                   
               
               
                   
                 IADENLTPEQVYNADETSLFWRYCPRKTLTTADET 
               
               
                   
                   
               
               
                   
                 APTGIKDAKDRITVLOCANAAGTHKCKLAVIGKSL 
               
               
                   
                   
               
               
                   
                 RPRCFQGVNFLPVHYYANKKAWITRDIFSDWFHKH 
               
               
                   
                   
               
               
                   
                 FVPAARAHCREAGLDDDCKILLFLDNCSAHPPAEI 
               
               
                   
                   
               
               
                   
                 LIKNNVYAMYFPPNVTSLIQPCDQGILRSMKSKYK 
               
               
                   
                   
               
               
                   
                 NTFLNSMLAAVNRGVGVEGFQKEFSMKDAIYAVAN 
               
               
                   
                   
               
               
                   
                 AWNTVTKDTVVHAWHNLWPATMFSDDDEQGGDFEG 
               
               
                   
                   
               
               
                   
                 FRMSSEKKMMSDLLTYAKNIPSESVSKLEEVDIEE 
               
               
                   
                   
               
               
                   
                 VFNIDNEAPVVHSLTDGEIAEMVLNQGDRDNSDDE 
               
               
                   
                   
               
               
                   
                 DDVVNTAEKVPIDDMVKMCDGLIEGLEQRAFITEQ 
               
               
                   
                   
               
               
                   
                 EIMSVYKIKERLLRQKPLLMR 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 173; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 185. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of CAGTAGTCCC CCCTTATCCG CGG (SEQ ID NO: 86), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of CCGCGGATAA GGGGGGACTA CTG (SEQ ID NO: 98), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 86; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 98. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 86; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 98. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 173; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 185. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 110, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 110. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of Tigger4) 
               
               
                   
                 SEQ ID NO: 110 
               
               
                   
                 MAPKRKSSDAGNSDMPKRSRKVLPLSEKVKVLDLI 
               
               
                   
                   
               
               
                   
                 RKEKKSYAEVAKIYGKNESSIREIVKKEKEIRASF 
               
               
                   
                   
               
               
                   
                 AVAPQTAKVTATVRDKCLVKMEKALNLWVEDMNRK 
               
               
                   
                   
               
               
                   
                 RVPIDGNVLRQKALSLYEDFSKGSPETSDTKPFTA 
               
               
                   
                   
               
               
                   
                 SKGWLHRFRNRFGLKNIKITGEAASANEEAAATFP 
               
               
                   
                   
               
               
                   
                 AELKKLIKEKGYHPKQVFNCDETGLFWKKMPNRTY 
               
               
                   
                   
               
               
                   
                 IHKSAKEAPGHKTWKDRLTLVLCGNAAGHMIKPGV 
               
               
                   
                   
               
               
                   
                 VYRAKNPRALKNKNKNYLPVFWQHNQKAWVIAILF 
               
               
                   
                   
               
               
                   
                 MEWFHQCFIPEVKKYLEEEGLLFKVLLIIDNAPGH 
               
               
                   
                   
               
               
                   
                 PESVCYENENVEVVFLPPNTTSLLQPLDQGIIRFV 
               
               
                   
                   
               
               
                   
                 KATYTRLVFDRIRSAIDADPNLDIMQCWKSFTIAD 
               
               
                   
                   
               
               
                   
                 AITFIKAAMDELKPETVNACWKNLWSEVVNDFKGF 
               
               
                   
                   
               
               
                   
                 PGIDGEVRKIIHAARQVGGEGFADMLDEEVEEHIE 
               
               
                   
                   
               
               
                   
                 GIIREVLTNEELEELVESSTEEEEDEEETEAEPAM 
               
               
                   
                   
               
               
                   
                 WTLPKFAEVFRIAQTLKDKIMEYDPRMERSIKVTR 
               
               
                   
                   
               
               
                   
                 MITEGLQPLQQHFDELKRKRQQLPITMFFQKISAK 
               
               
                   
                   
               
               
                   
                 NLQLSRIPNHRHRLLLTSNHRHRHGSMIQDHPKQM 
               
               
                   
                   
               
               
                   
                 ILLLTYRQKVNSSLTLRHNAYVIHLTSSHHVGIVS 
               
               
                   
                   
               
               
                   
                 SHIITRRRVSTVQ 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 175; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 187. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of TAGGG (SEQ ID NO: 87), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of CCCTA (SEQ ID NO: 99), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 87; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 99. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 87; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 99. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of TTTATAAT (SEQ ID NO: 204), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 204. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 175; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 1000) sequence identity to the nucleic acid sequence of SEQ ID NO: 187. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 111, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 111. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of BAT-19_Crp) 
               
               
                   
                 SEQ ID NO: 111 
               
               
                   
                 MNAKKSSKTAKYVSALDRARQYPAGTLHADGGRLF 
               
               
                   
                   
               
               
                   
                 CTSCNVTLDVSRKTSIDRHLESEAHMKRKAAAEAE 
               
               
                   
                   
               
               
                   
                 GQSKKQATVSSLFKRTTESSLARREAAFSLVEAFA 
               
               
                   
                   
               
               
                   
                 AANIPLEKLDHPKLRDYLNQNVPNAGSFPRANKLR 
               
               
                   
                   
               
               
                   
                 QDYLPAVIASHVQSTKAALAEYHSFSIVADEATDP 
               
               
                   
                   
               
               
                   
                 QDNYVLHILFVPGSWTDRDLPVFQTEPVHLTSVNF 
               
               
                   
                   
               
               
                   
                 STVSQAIVKVIVSYGVDFNKISAFLSDNAMYMCKA 
               
               
                   
                   
               
               
                   
                 FSDVLQGMLPNAVHVTCNAHILSLVSDTWRTQFPE 
               
               
                   
                   
               
               
                   
                 VDSLVSSFKKAFKHCPGRKVRYRESVASQSGDSHG 
               
               
                   
                   
               
               
                   
                 TVPLPPEPVLTRWNSWFNAVRYHAKHMQHYQQFVS 
               
               
                   
                   
               
               
                   
                 EELEITPSTQCMLKLRDLLGRGDIQDQVTFVADNA 
               
               
                   
                   
               
               
                   
                 TRFMELLTWFEGRQAVIHHAHNKLMDLISWVEDRA 
               
               
                   
                   
               
               
                   
                 SQELSSCPRENQRRRDLFQAIAVKLNQYYRYDPSL 
               
               
                   
                   
               
               
                   
                 PPRFRQPAAPFLSAVRILDPNQVPLLSCDPQLLKA 
               
               
                   
                   
               
               
                   
                 IPGWGKEHDNERAAYVNFVKECQMPVPVPVFWDSV 
               
               
                   
                   
               
               
                   
                 CDRFPHLYALARRCLTIPTNSVDAERAISVYGQVF 
               
               
                   
                   
               
               
                   
                 TPQRQRMSPATAAGCSMLAFNT 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 176; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 188. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of TAGG (SEQ ID NO: 88), a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of CCTA (SEQ ID NO: 100), a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 88; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 100. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 88; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 100. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of CTATATAG (SEQ ID NO: 205), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 205. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 176; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 188. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 112, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 112. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase of hAT-17B_Croc) 
               
               
                   
                 SEQ ID NO: 112 
               
               
                   
                 MTVSSTPISSPSVSLPVPEEELDLEAGDLSALAKE 
               
               
                   
                   
               
               
                   
                 ILGTEGESEFVLHTPQSSPRARSPSPEGTSEEVEV 
               
               
                   
                   
               
               
                   
                 VEASGSAESAPPVVVPLPAEEGDKAGSPPSTQKRG 
               
               
                   
                   
               
               
                   
                 GSVVWDHFEVADDPKYAICQHCRRQISRGKEVKHF 
               
               
                   
                   
               
               
                   
                 TTTAMLLHLRRQHPLALA 
               
               
                   
                   
               
               
                   
                 PPQPGTSGSVPKGKSPSRSKAPVPTKQRQATLERW 
               
               
                   
                   
               
               
                   
                 GKAADKGGRVARASEVTQSIGEMLALDGQPFSLVE 
               
               
                   
                   
               
               
                   
                 RPGFRRLMALVAPSYQVPARTTFSRTVVPSLYEAC 
               
               
                   
                   
               
               
                   
                 REYLREELRKAGPQVALHFTSDIWSSRGGDHAYLS 
               
               
                   
                   
               
               
                   
                 LTGHWCDQSGRRWALLQAEVMDESHTAREIMVAMN 
               
               
                   
                   
               
               
                   
                 RMVQGWLVGQGELTRGFMVTDNGANMVKAVRDANF 
               
               
                   
                   
               
               
                   
                 VGIRCVAHKLHLIVRDALEGDRAAGDGAVTTTQLI 
               
               
                   
                   
               
               
                   
                 SKCRKVAGYFHRSIKGGKMLRDKQAELSVPQHKIM 
               
               
                   
                   
               
               
                   
                 QDVETRWNSTYLMLERLVEQQKAIHEMALLGEIGI 
               
               
                   
                   
               
               
                   
                 SGPLNKAEWDTISQILVVLKPFLEATETLSAGDAL 
               
               
                   
                   
               
               
                   
                 LSQVIPVVRELENQMEKFQGIDVPGWGKPLSPEVQ 
               
               
                   
                   
               
               
                   
                 ALVKRLKEGIKKWLDPLRSSTVHVLAGMCDPRVKG 
               
               
                   
                   
               
               
                   
                 SMCTGSTKTLDHWTEVLVNRVREAEGQRRGDVEEG 
               
               
                   
                   
               
               
                   
                 DPLSRASTPSTSQSPPRQPLPMWAKGMASMLGSRR 
               
               
                   
                   
               
               
                   
                 TRPQPRAGSAEASVATYLAEDVEPLDCDPLAYWAT 
               
               
                   
                   
               
               
                   
                 RSQMWQDLATVAREHLSCPPTSVPSERVFSIAGDV 
               
               
                   
                   
               
               
                   
                 VTPHRTSLDPGLV 
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 177; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 189. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 89, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 101, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 89; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 101. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 89; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 101. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of ATTAATAG (SEQ ID NO: 206), and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 206. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 177; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 189. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 113, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 113. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (transposase ofhAT-10_XT) 
               
               
                   
                 SEQ ID NO: 113 
               
               
                   
                 MTGRGRGRKGGMGGKGGEGGRKGGVGGEGGRKGGV 
               
               
                   
                   
               
               
                   
                 GGEGGVGGKGGLGGRSRGAGCGQEPSTRAMPSLGN 
               
               
                   
                   
               
               
                   
                 IGQQPQQQSQQQQQQLQQQQVAGRSGATGSRKTLP 
               
               
                   
                   
               
               
                   
                 DFFATTSRPIQ 
               
               
                   
                   
               
               
                   
                 SQQAEAVMDWLTQATSSAADSSEQQEVASPASSVI 
               
               
                   
                   
               
               
                   
                 RGMLSPLLFDPEVDFSPDTQDLFDDQTGMGGAFLS 
               
               
                   
                   
               
               
                   
                 DEEEVYVAQHTTSAVGDVGQGPLMAGQQADEPDVD 
               
               
                   
                   
               
               
                   
                 VDAGSDLGEDVEDDGDRSWVPAQEDNSSSSEGELC 
               
               
                   
                   
               
               
                   
                 VVVSDADEEPAPKKQKTPPTTARGGRQQGSTAPGS 
               
               
                   
                   
               
               
                   
                 TGSGRMGRDLQPSGPQPSTAGKVVARTSAVWAFFT 
               
               
                   
                   
               
               
                   
                 CQSQDPSVAVCHLCRQKVRRGQTGSHMGTSALSSH 
               
               
                   
                   
               
               
                   
                 MKRHHRMTWEQHQSGRAPGGSGASCPPPPIPQGTG 
               
               
                   
                   
               
               
                   
                 ASSPIPVAREEDSEAHSWRRPLCSSASSAAPSVSS 
               
               
                   
                   
               
               
                   
                 SSLLPSAQPLSRQVSLPQFLGRKAPLSSSHPQVQR 
               
               
                   
                   
               
               
                   
                 LNACLAKLLAVQLLPYQLVDAAPFRQLMACAAPNW 
               
               
                   
                   
               
               
                   
                 RIPSRHYFARKAVPALHQQVVDNVSLSLDYAVGGR 
               
               
                   
                   
               
               
                   
                 VHCTTDTWTSRHGQGRYISYTAHWVTLMSAGEGAG 
               
               
                   
                   
               
               
                   
                 SRTPLRLEVPPRGVKGKPHTATSSSSSSTMDEPPL 
               
               
                   
                   
               
               
                   
                 KRPRSYASVQYKRCQAVLQLLCLGERSHTAPELHA 
               
               
                   
                   
               
               
                   
                 AFQTQVQRWFTPRQLQAGNVVCDNGRNLLAALHLG 
               
               
                   
                   
               
               
                   
                 RLTHVPCFAHVLNLVVQHFLKSYTGLGVVLEKARR 
               
               
                   
                   
               
               
                   
                 VCGHFRRSPTASASLSRMQRQHSLPPHRLICDLPT 
               
               
                   
                   
               
               
                   
                 RWNSTLHMVERLVEQRWAVSNYLLEHSARGTQGAN 
               
               
                   
                   
               
               
                   
                 LGYFSTEQWQQMRQLCRVLAPFEQATRFVSRDNAC 
               
               
                   
                   
               
               
                   
                 VSDIIPLVFLLKRTLDGLLEEGDVPEEEECRPRRQ 
               
               
                   
                   
               
               
                   
                 VEGAARHDEEDMPNEEDEGEEDWVPAEQGEQGPRH 
               
               
                   
                   
               
               
                   
                 PTTPAVVRGWEDTEQGEEAGDDLLHLQGSQEEVGR 
               
               
                   
                   
               
               
                   
                 GHLFYMAAHMQTCLRSDSRICAIKDREDYWVATMI 
               
               
                   
                   
               
               
                   
                 DPRYKEKMAQFLVPSQRERRVGQLKRALCAKLMEA 
               
               
                   
                   
               
               
                   
                 FPQPDTQASTTPHIQQRQESSSSSSSAARGGGNLM 
               
               
                   
                   
               
               
                   
                 DVWRSFFEPRQAAAGLVSTQSHHQQRLDNMVADYM 
               
               
                   
                   
               
               
                   
                 GSVSVQDAMHTDDDPMNFWVSRLDQWPELAQYALE 
               
               
                   
                   
               
               
                   
                 VLACPPASVLSERVFSAAGGVVTEKRTRLSTGSVD 
               
               
                   
                   
               
               
                   
                 RLSFIKMNEAWISGDIHVPIADIRD  
               
            
           
         
       
     
     In some embodiments, the transposable element comprises: 1) a 5′TR in a LTF comprising the nucleic acid sequence of SEQ ID NO: 178; and 2) a 3′TR in a RTF comprising the nucleic acid sequence of SEQ ID NO: 190. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 90, a variant thereof, or a fragment thereof; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 102, a variant thereof, or a fragment thereof. In some embodiments, the transposable element comprises: 1) a 5′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 90; and 2) a 3′TR having a nucleic acid sequence that has at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more) sequence identity to the nucleic acid sequence of SEQ ID NO: 102. In some embodiments, the transposable element comprises: 1) a 5′TR comprising the nucleic acid sequence of SEQ ID NO: 90; and 2) a 3′TR comprising the nucleic acid sequence of SEQ ID NO: 102. In some embodiments, the transposable element further comprises a 5′TSD comprising the nucleic acid sequence of SEQ ID NO: 193, and a 3′TSD comprising the nucleic acid sequence of SEQ ID NO: 193. In some embodiments, the transposable element does not comprise 5′TSD and/or 3′TSD. In some embodiments, the transposable element comprises a LTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 178; and 2) a RTF comprising a nucleic acid sequence having at least about 90% (e.g., at least about any one of 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%) sequence identity to the nucleic acid sequence of SEQ ID NO: 190. In some embodiments, the transposable element is associated with a transposase comprising the amino acid sequence of SEQ ID NO: 114, or a variant thereof. In some embodiments, the transposase comprises an amino acid sequence having at least about 80% (e.g., at least about any one of 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more) sequence identity to the amino acid sequence of SEQ ID NO: 114. In some embodiments, the transposable element comprises a nucleic acid sequence encoding the transposase. In some embodiments, the transposable element does not comprise a nucleic acid sequence encoding the transposase. 
     
       
         
           
               
               
            
               
                   
                 (5′TR of MARWOLEN1) 
               
               
                   
                 SEQ ID NO: 90 
               
               
                   
                 TACAGTGGCGAGCAAAACTGAGTGCATGTTCACAA 
               
               
                   
                   
               
               
                   
                 CTCTCACTTTGGCCATCAATAAAAACACAACCGCT 
               
               
                   
                   
               
               
                   
                 TATGCAAATTCAATAATTTTTTTTATATTATTAAT 
               
               
                   
                   
               
               
                   
                 CTTAATTAATTTATAACTTATTTTATGAGAAAAAA 
               
               
                   
                   
               
               
                   
                 CAAATTTAGCATATATATTCAAAGACTTAGAATAA 
               
               
                   
                   
               
               
                   
                 AAAAACTAAATAAACTCACATAAACTCAATTTTGC 
               
               
                   
                   
               
               
                   
                 ACGTTT 
               
               
                   
                   
               
               
                   
                 (3′TR of MARWOLEN1) 
               
               
                   
                 SEQ ID NO: 102 
               
               
                   
                 AAACGTGCAAAATTGAGTTTATGTGAGTTTATTTA 
               
               
                   
                   
               
               
                   
                 GTTTTTTTATTCTAAGTCTTTGAATATATATGCTA 
               
               
                   
                   
               
               
                   
                 AATTTGTTTTTTCTCATAAAATAAGTTATAAATTA 
               
               
                   
                   
               
               
                   
                 ATTAAGATTAATAATATAAAAAAAATTATTGAATT 
               
               
                   
                   
               
               
                   
                 TGCATAAGCGGTTGTGTTTTTATTGATGGCCAAAG 
               
               
                   
                   
               
               
                   
                 TGAGAGTTGTGAACATGCACTCAGTTTTGCTCGCC 
               
               
                   
                   
               
               
                   
                 ACTGTA 
               
               
                   
                   
               
               
                   
                 (transposase of MARWOLENI) 
               
               
                   
                 SEQ ID NO: 114 
               
               
                   
                 MRKLSGEIQNNIVSLTESGNSTRQIAERLGISQSA 
               
               
                   
                   
               
               
                   
                 VVSIQKRRKLTPKPQVSGRKKLLKDSDARLMMSEM 
               
               
                   
                   
               
               
                   
                 RKNKNLTPKGACLAINKNVSEWTARRALQDIGYMS 
               
               
                   
                   
               
               
                   
                 IVKKNKPALSDKNVKARLKFAKDHKNWTIDDWKRV 
               
               
                   
                   
               
               
                   
                 VWSDESKFNRFQSDGKQYCWIRPGDRVQRHHVKQT 
               
               
                   
                   
               
               
                   
                 VKHGGGNIMVWGCFTWWHIGPLQLVEGIMKKEDYL 
               
               
                   
                   
               
               
                   
                 RILQTNLPNYFDKCAYPEKDIIFQQDGDPKIITAK 
               
               
                   
                   
               
               
                   
                 IVKEWIGKQHFQLMEWPAQSPDLNPIENLWSIVKR 
               
               
                   
                   
               
               
                   
                 RLGQYDSAPKNMGDLWERVAVEWSRIPQDILRNLV 
               
               
                   
                   
               
               
                   
                 ESMPKRVTEVTVNKGLWTKY 
               
            
           
         
       
     
     Heterologous Nucleic Acid 
     The engineered transposable elements described herein are suitable for transposing a variety of heterologous nucleic acids. In some embodiments, the heterologous nucleic acid is a DNA. In some embodiments, the heterologous nucleic acid is double-stranded. In some embodiments, the heterologous nucleic acid comprises one or more modified nucleotides. In some embodiments, the heterologous nucleic acid is not modified. 
     The heterologous nucleic acid in the transposable element may be of various suitable lengths. In some embodiments, the heterologous nucleic acid is at least about any one of 1 kb, 2 kb, 10 kb, 20 kb, 30 kb, 40 kb, 50 kb, 60 kb, 70 kb, 80 kb, 90 kb, 100 kb, 150 kb, 200 kb, 250 kb, 300 kb, 350 kb, 400 kb, 450 kb, 500 kb, 600 kb, 700 kb, 800 kb, 900 kb, 1000 kb or more in length. In some embodiments, the heterologous nucleic acid is no more than about any one of 1000 kb, 900 kb, 800 kb, 700 kb, 600 kb, 500 kb, 450 kb, 400 kb, 350 kb, 300 kb, 250 kb, 200 kb, 150 kb, 100 kb, 90 kb, 80 kb, 70 kb, 60 kb, 50 kb, 40 kb, 30 kb, 20 kb, 10 kb, 5 kb, 2 kb, or 1 kb in length. In some embodiments, the heterologous nucleic acid has a length in any one of the ranges from about 100 bp to about 1 kb, about 1 kb to about 2 kb, about 2 kb to about 5 kb, about 5 kb to about 10 kb, about 100 bp to about 5 kb, about 100 bp to about 2 kb, about 2 kb to about 10 kb, about 1 kb to about 10 kb, about 10 kb to about 20 kb, about 20 kb to about 50 kb, about 50 kb to about 100 kb, about 1 kb to about 100 kb, about 150 kb to about 200 kb, about 200 kb to about 300 kb, about 300 kb to about 400 kb, about 400 kb to about 500 kb, about 500 kb to about 600 kb, about 600 kb to about 700 kb, about 700 kb to about 800 kb, about 800 kb to about 900 kb, about 900 kb to about 1000 kb, about 10 kb to about 100 kb, about 100 kb to about 500 kb, about 500 kb to about 1000 kb, or about 10 kb to about 500 kb. In some embodiments, the heterologous nucleic acid is from about 10 kb to about 300 kb nucleotides long. In some embodiments, the heterologous nucleic acid is from about 100 bp to about 300 kb nucleotides long. 
     The heterologous nucleic acid may comprise comprises one or more coding sequences, including any one of 1, 2, 3, 4, 5, 6, 10 or more coding sequences. Any suitable coding sequence may be used in the present application, and the coding sequence may encode any suitable biological product of interest. In some embodiments, the coding sequence encodes an RNA molecule. In some embodiments, the coding sequence encodes a polypeptide, such as a protein. In some embodiments, the heterologous nucleic acid comprises a first coding sequence encoding a first protein and a second coding sequence encoding a second protein. In some embodiments, the heterologous nucleic acid comprises a first coding sequence encoding a first RNA and a second coding sequence encoding a second RNA. In some embodiments, the heterologous nucleic acid comprises a first coding sequence encoding a protein and a second coding sequence encoding a RNA. 
     In some embodiments, the coding sequence encodes a therapeutic protein. In some embodiments, the coding sequence encodes a therapeutic antibody, including monoclonal antibody, multispecific antibody, and antibody fragments. In some embodiments, the coding sequence encodes a cytokine. In some embodiments, the coding sequence encodes an antigen. In some embodiments, the coding sequence encodes a therapeutic agent useful in gene therapy. Exemplary therapeutic proteins useful for gene therapy include, but are not limited to, adenosine deaminase, the enzymes affected in lysosomal storage diseases, apolipoprotein E, brain derived neurotropihic factor (BDNF), bone morphogenetic protein 2 (BMP-2), bone morphogenetic protein 6 (BMP-6), bone morphogenetic protein 7 (BMP-7), cardiotrophin 1 (CT-1), CD22, CD40, ciliary neurotrophic factor (CNTF), CCL1-CCL28, CXCL1-CXCL17, CXCL1, CXCL2, CX3CL1, vascular endothelial cell growth factor (VEGF), dopamine, erythropoietin, Factor IX, Factor VIII, epidermal growth factor (EGF), estrogen, FAS-ligand, fibroblast growth factor 1 (FGF-1), fibroblast growth factor 2 (FGF-2), fibroblast growth factor 4 (FGF-4), fibroblast growth factor 5 (FGF-5), fibroblast growth factor 6 (FGF-6), fibroblast growth factor 1 (FGF-7), fibroblast growth factor 1 (FGF-10), Flt-3, granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage stimulating factor (GM-CSF), growth hormone, hepatocyte growth factor (HGF), interferon alpha (IFN-a), interferon beta (IFN-b), interferon gamma (IFNg), insulin, glucagon, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), interleukin 1 (IL-1), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 9 (IL-9), interleukin 10 (IL-10), interleukin 11 (IL-11), interleukin 12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15), interleukin 17 (IL-17), interleukin 19 (IL-19), macrophage colony-stimulating factor (M-CSF), monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein 3a (MIP-3a), macrophage inflammatory protein 3b (MIP-3b), nerve growth factor (NGF), neurotrophin 3 (NT-3), neurotrophin 4 (NT-4), parathyroid hormone, platelet derived growth factor AA (PDGF-AA), platelet derived growth factor AB (PDGF-AB), platelet derived growth factor BB (PDGF-BB), platelet derived growth factor CC (PDGF-CC), platelet derived growth factor DD (PDGF-DD), RANTES, stem cell factor (SCF), stromal cell derived factor 1 (SDF-1), transforming growth factor alpha (TGF-a), transforming growth factor beta (TGF-b), tumor necrosis factor alpha (TNF-a), Wnt1, Wnt2, Wnt2b/13, Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt5b, Wnt6, Wnt7a, Wnt7b, Wnt7c, Wnt8, Wnt8a, Wnt8b, Wnt8c, Wnt10a, Wnt10b, Wnt11, Wnt14, Wnt15, or Wnt16, Sonic hedgehog, Desert hedgehog, and Indian hedgehog. 
     In some embodiments, the coding sequence encodes an engineered receptor, such as a chimeric antigen receptor (CAR) or an engineered T-cell receptor (TCR). 
     As used herein, “Chimeric antigen receptor” or “CAR” refers to genetically engineered receptors, which graft one or more antigen specificity onto cells, such as T cells. CARs are also known as “artificial T-cell receptors,” “chimeric T cell receptors,” or “chimeric immune receptors.” In some embodiments, the CAR comprises an extracellular variable domain of an antibody specific for a tumor antigen, and an intracellular signaling domain of a T cell or other receptors, such as one or more costimulatory domains. “CAR-T” refers to a T cell that expresses a CAR. 
     In some particular embodiments, the coding sequence encodes a chimeric antigen receptor (CAR). Many chimeric antigen receptors are known in the art and may be suitable for use in the present application. CARs can also be constructed with a specificity for any cell surface marker by utilizing antigen binding fragments or antibody variable domains of, for example, antibody molecules. Any methods for producing a CAR may be used herein. See, for example, U.S. Pat. Nos. 6,410,319, 7,446,191, 7,514,537, 9,765,342B2, WO 2002/077029, WO2015/142675, US2010/065818, US 2010/025177, US 2007/059298, and Berger C. et al., J. Clinical Investigation 118:1 294-308 (2008), which are hereby incorporated by reference. 
     471 “T cell receptor” or “TCR” as used herein refers to endogenous or recombinant T cell receptor comprising an extracellular antigen binding domain that binds to a specific antigenic peptide bound in an MHC molecule. In some embodiments, the TCR comprises a TCRα polypeptide chain and a TCR β polypeptide chain. In some embodiments, the TCR specifically binds a tumor antigen. “TCR-T” refers to a T cell that expresses a recombinant TCR. The term “recombinant” refers to a biomolecule, e.g., a gene or protein, that (1) has been removed from its naturally occurring environment, (2) is not associated with all or a portion of a polynucleotide in which the gene is found in nature, (3) is operatively linked to a polynucleotide which it is not linked to in nature, or (4) does not occur in nature. The term “recombinant” can be used in reference to cloned DNA isolates, chemically synthesized polynucleotide analogs, or polynucleotide analogs that are biologically synthesized by heterologous systems, as well as proteins and/or mRNAs encoded by such nucleic acids. 
     In some particular embodiments, the coding sequence encodes an engineered T-cell receptor (TCR). In some embodiments, the engineered TCR is specific for a tumor antigen. In some embodiments, the tumor antigen is derived from an intracellular protein of tumor cells. Many TCRs specific for tumor antigens (including tumor-associated antigens) have been described, including, for example, NY-ESO-1 cancer-testis antigen, the p53 tumor suppressor antigens, TCRs for tumor antigens in melanoma (e.g., MARTI, gp100), leukemia (e.g., WT1, minor histocompatibility antigens), and breast cancer (HER2, NY-BR1, for example). Any of the TCRs known in the art may be used in the present application. In some embodiments, the TCR has an enhanced affinity to the tumor antigen. Exemplary TCRs and methods for producing TCRs have been described, for example, in U.S. Pat. No. 5,830,755, and Kessels et al. Immunotherapy through TCR gene transfer.  Nat. Immunol.  2, 957-961 (2001). 
     In some embodiments, the coding sequence encodes a selectable marker. A “selectable marker” is a gene, the expression of which creates a detectable phenotype and which facilitates detection of host cells having the heterologous nucleic acid encoding the selectable marker inserted in a target nucleic acid (e.g., genomic DNA). In some embodiments, the selectable marker confers resistance to an antibiotic agent, such as puromycin. Additional non-limiting examples of selectable markers include drug resistance genes and nutritional markers. For example, the selectable marker can be a gene that confers resistance to an antibiotic selected from the group consisting of: ampicillin, kanamycin, erythromycin, chloramphenicol, gentamycin, kasugamycin, rifampicin, spectinomycin, D-Cycloserine, nalidixic acid, streptomycin, or tetracycline. Other non-limiting examples of selection markers include adenosine deaminase, aminoglycoside phosphotransferase, dihydrofolate reductase, hygromycin-B-phosphotransferase, thymidine kinase, and xanthine-guanine phosphoribosyltransferase. Examples of selectable markers suitable for mammalian cells also include DHFR, thymidine kinase, metallothionein-I and -II, preferably primate metallothionein genes, adenosine deaminase, ornithine decarboxylase, etc. In some particular embodiments, the heterologous nucleic acid comprises a coding sequence of a puromycin resistance gene. 
     In some embodiments, the coding sequence is a reporter gene. A “reporter gene” is a gene that encodes a detectable product so that detection of the reporter gene product can be used to evaluate the function of a nucleic acid of interest. A reporter gene may be fused to any suitable nucleic acid of interest (e.g. promoter, a gene of interest, a selectable marker, and/or terminal repeats of a transposable element) to allow one to detect whether the nucleic acid of interest is expressed or altered (e.g. excised by a transposase) under a given set of conditions. Non-limiting examples of reporter genes include: 3-galactosidase, 3-glucuronidase, glutathione-S-transferase (GST), horseradish peroxidase (HRP), luciferase, chloramphenicol acetyltransferase (CAT), secreted alkaline phosphatase (SEAP), green fluorescent protein (GFP, e.g., eGFP), red fluorescent protein (RFP), HcRed, DsRed, cyan fluorescent protein (CFP), yellow fluorescent protein (YFP), catechol 2,3-oxygenase (xylE), and autofluorescent proteins including blue fluorescent protein (BFP). In some embodiments, the heterologous nucleic acid comprises a coding sequence encoding an enhanced green fluorescent protein (eGFP). In some embodiments, the coding sequence encodes more than one biological products, or the coding sequence may encode a fusion protein. In some embodiments, the heterologous nucleic acid of the present application comprises a coding sequence encoding a puromycine resistance—enhanced green fluorescent protein (eGFP) fusion protein. 
     In some embodiments, the coding sequence encodes a transposase. 
     In some embodiments, the coding sequence encodes a polypeptide useful in genome editing. Genome editing may be accomplished by using nucleases, which create specific double-strand breaks (DSBs) at desired locations in the genome, and harness the cell&#39;s endogenous mechanisms to repair the induced break by homology-directed repair (HDR) (e.g., homologous recombination) or by nonhomologous end joining (NHEJ). Any suitable nuclease may be introduced into a cell to induce genome editing of a target DNA sequence including, but not limited to, CRISPR-associated protein (Cas, e.g., Cas9) nucleases, zinc finger nucleases (ZFNs, e.g. FokI), transcription activator-like effector nucleases (TALENs, e.g., TALEs), meganucleases, and variants thereof (Shukla et al. (2009) Nature 459: 437-441; Townsend et al (2009) Nature 459: 442-445). In some embodiments, the coding sequence encodes a Cas9 polypeptide. 
     In some embodiments, the coding sequence encodes an RNA molecule. The RNA molecule may be a protein-coding RNA such as messenger RNA (mRNA), or a non-protein coding RNA, including, but not limited to, transfer RNA (tRNA) and ribosomal RNA (rRNA), small RNA such as microRNA (miRNA), small interfering RNA (siRNA), short hairpin RNA (shRNA), or piwi-interacting RNA (piRNA), and long non-coding RNA (lincRNA). Certain types of small RNA, such as microRNA and siRNA, are important in the process RNA interference (RNAi). RNAi is a process of genetic regulation in which a target gene that would otherwise normally express is suppressed from expression due to interference of small RNAs through post-transcriptional degradation or inhibition of translation. For detailed description of RNAi techniques, see, e.g., U.S. Pat. Nos. 5,034,323; 6,326,527; 6,452,067; 6,573,099; 6,753,139; and 6,777,588. In some embodiments, the coding sequence encodes a regulatory RNA. In some embodiments, the coding sequence encodes an RNAi molecule. In some embodiments, the coding sequence encodes a shRNA. In some embodiments, the coding sequence encode an miRNA. 
     In some embodiments, the coding sequence encodes an RNA molecule that is useful in genome editing. Examples of such RNA molecules include, but are not limited to, CRISPR RNA (crRNA), trans-activating crRNA (tracrRNA), guide RNA (gRNA), and single guide RNA (sgRNA). 
     In some embodiments, the heterologous nucleic acid further comprises one or more regulatory elements regulating expression of the coding sequence. Regulatory elements are contemplated for use with the methods and constructs described herein. The term “regulatory element” is intended to include promoters, enhancers, internal ribosomal entry sites (IRES), and other expression control elements (e.g., transcription termination signals, such as polyadenylation (poly-A) signals and poly-U sequences). Such regulatory elements are described, for example, in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990). 
     Promoters are an important regulatory element that directs expression pattern of the coding sequence. In some embodiments, the heterologous nucleic acid comprises a promoter operably linked to the coding sequence. Any suitable promoters may be used in the present application. In some embodiments, the promoter is an endogenous promoter. In some embodiments, the promoter is a heterologous promoter. Varieties of promoters have been explored for gene expression in mammalian cells, and any of the promoters known in the art may be used in the present application. Promoters may be roughly categorized as constitutive promoters or regulated promoters, such as inducible promoters. In some embodiments, the heterologous nucleic acid comprises a coding sequence (e.g., transposase-coding sequence) operably linked to a constitutive promoter. In some embodiments, the heterologous nucleic acid comprises a coding sequence (e.g., transposase-coding sequence) operably linked to an inducible promoter. 
     Constitutive promoters allow a heterologous nucleic acid to be expressed constitutively in the host cells. Exemplary constitutive promoters contemplated herein include, but are not limited to, cytomegalovirus (CMV) promoters, human elongation factors-1alpha (hEF1α), ubiquitin C promoter (UbiC), phosphoglycerokinase promoter (PGK), simian virus 40 early promoter (SV40), and chicken β-Actin promoter coupled with CMV early enhancer (CAGG). The efficiencies of such constitutive promoters on driving transgene expression have been widely compared in a huge number of studies. For example, Michael C. Milone et al compared the efficiencies of CMV, hEF1α, UbiC and PGK to drive chimeric antigen receptor expression in primary human T cells, and concluded that hEF1α promoter not only induced the highest level of transgene expression, but was also optimally maintained in the CD4 and CD8 human T cells (Molecular Therapy, 17(8): 1453-1464 (2009)). In some embodiments, the promoter in the heterologous nucleic acid is a CAG promoter. Exemplary engineered transposable elements comprising a heterologous nucleic acid sequence encoding a transposase or a selectable marker/reporter driven by a constitutive promoter are shown in  FIG.  3   , wherein the promoter is a CMV promoter or a PGK promoter. 
     It is contemplated that using a promoter with a moderate or weak expression pattern, as opposed to a strong expression promoter (e.g. the CMV promoter), of the coding sequence may be desired for certain applications, such as in certain gene therapies, in order to avoid or reduce events of transposase-based autoregulation, collectively referred to as overproduction inhibition (OPI). 
     Regulated promoters, such as inducible promoters, allow a heterologous nucleic acid to be expressed under certain circumstances, such as in a specific developmental stage, or a specific tissue type or subcellular location. Various types of regulated promoters are known in the art, including inducible, tissue-specific, cell-type-specific, or cell cycle-specific, see e.g., Sambrook and Russell, 2001. Inducible promoters belong to the category of regulated promoters. An inducible promoter can be induced by one or more conditions, such as a physical condition, microenvironment of the engineered mammalian cell, or the physiological state of the engineered mammalian cell, an inducer (i.e., an inducing agent), or a combination thereof. 
     In some embodiments, it may be desirable to use a promoter to express the coding sequence in only a subset of cell types, cell lineages, or tissues or during specific stages of development. Examples include, but are not limited to: an B29 promoter (B cell expression), a runt transcription factor (CBFa2) promoter (stem cell expression), an CD14 promoter (monocytic cell expression), an CD43 promoter (leukocyte and platelet expression), an CD45 promoter (hematopoietic cell expression), an CD68 promoter (macrophage expression), an endoglin promoter (endothelial cell expression), a fms-related tyrosine kinase 1 (FLT1) promoter (endothelial cell expression), an integrin, alpha 2b (ITGA2B) promoter (megakaryocyte expression), an intracellular adhesion molecule 2 (ICAM-2) promoter (endothelial cell expression), an interferon beta (IFN-β) promoter (hematopoietic cell expression), a β-globin LCR (erythroid cell expression), a globin promoter (erythroid cell expression), a β-globin promoter (erythroid cell expression), an α-globin HS40 enhancer (erythroid cell expression), an ankyrin-1 promoter (erythroid cell expression), and a Wiskott-Aldrich syndrome protein (WASP) promoter (hematopoietic cell expression). 
     Aspects of the methods described herein may make use of terminator sequences. A terminator sequence includes a section of nucleic acid sequence that marks the end of a gene or an operon during transcription. This sequence mediates transcriptional termination by providing signals in the newly synthesized mRNA that trigger processes to release the mRNA from the transcriptional complex. These processes include the direct interaction of the mRNA secondary structure with the complex and/or the indirect activities of recruited termination factors. Release of the transcriptional complex frees RNA polymerase and related transcriptional machinery to begin transcription of new mRNAs. Terminator sequences include those known in the art. In some embodiments of the present application, the terminator sequence is a polyadenylation (poly-A) signal. 
     In some embodiments, the heterologous nucleic acid comprises at least one restriction endonuclease recognized site, e.g. restriction site, serving as a site for insertion of an exogenous nucleic acid. A variety of restriction sites are known in the art and include, but are not limited to: HindIII, PstI, SalI, AccI, HincII, XbaI, BamHI, SmaI, XmaI, KpnI, SacI, EcoRI, and the like. In some embodiments, the restriction site is a multiple cloning site (MCS, also known as a polylinker), i.e. a closely arranged series or array of sites recognized by a plurality of different restriction enzymes, such as those listed above. In other embodiments, the heterologous nucleic acid of the present application comprises recombinase recognition sites, such as LoxP, FRT, or AttB/AttP sites, which are recognized by the Cre, Flp, and PhiC31 recombinases, respectively. 
     In some embodiments, the heterologous nucleic acid comprises a tag sequence. A tag sequence can be used to identify a molecule, or provide a site for capture of a molecule, e.g., by hybridization. 
     In some embodiments, the heterologous nucleic acid comprises a barcode sequence. “Barcode sequence” refers to a nucleic acid having a sequence, which can be used to identify and/or distinguish one or more first molecules to which the nucleic acid barcode is conjugated from one or more second molecules. Nucleic acid barcode sequences are typically short, e.g., about 5 to 20 bases in length, and may be conjugated to one or more target molecules of interest or amplification products thereof. Nucleic acid barcode sequences may be single or double stranded. 
     In some embodiments, the heterologous nucleic acid comprises a unique molecular identifier (UMI). The term “unique molecular identifier” or “UMI” as used herein refers to nucleic acid sequence, which can be used to identify and/or distinguish one or more first molecules to which the UMI is conjugated from one or more second molecules. UMIs are typically short, e.g., about 5 to 20 bases in length, and may be conjugated to one or more target molecules of interest or amplification products thereof. UMIs may be single or double stranded. In some embodiments, both a nucleic acid barcode sequence and a UMI are incorporated into a nucleic acid target molecule or an amplification product thereof. Generally, a UMI is used to distinguish between molecules of a similar type within a population or group, whereas a nucleic acid barcode sequence is used to distinguish between populations or groups of molecules. In some embodiments, where both a UMI and a nucleic acid barcode sequence are utilized, the UMI is shorter in sequence length than the nucleic acid barcode sequence. In some embodiments, where both a UMI and a nucleic acid barcode sequence are utilized, the UMI is incorporated into the target nucleic acid or an amplification product thereof prior to the incorporation of the nucleic acid barcode sequence. In some embodiments, where both a UMI and a nucleic acid barcode sequence are utilized, the nucleic acid barcode sequence is incorporated into the UMI or an amplification product thereof subsequent to the incorporation of the UMI into a target nucleic acid or an amplification product thereof. 
     Transposition Activity 
     In some embodiments, the transposable element of the present application exhibits transposition activity in vitro or in a cell. 
     Transposition activity can be detected by a number of techniques known to an ordinary skilled person in the art. Examples of assays for measuring the excision of a transposable element from a vector, the integration of a transposon into the genomic or extrachromosomal DNA of a cell, and the ability of transposase to bind to an inverted repeat may be found in, for instance, Ivies et al. Cell, 91, 501-510 (1997), WO 98/40510 (Hackett et al.), WO 99/25817 (Hackett et al.), and WO00/68399 (Mclvor et al.). 
     In some embodiments, the transposition assay is based on trans-complementation of two components in a transposable element system, with one component containing a selectable marker/reporter gene (donor) flanked by terminal repeats, and another component that expresses the transposase that recognizes and binds to the terminal repeats to perform transposition (helper). By way of example,  FIG.  3    shows an exemplary set of binary constructs for use in screening active transposable elements. The top construct is the helper construct for transposase expression, comprising from 5′ to 3′: a cytomegalovirus (CMV) promoter, a transposase (Tn) gene, and a poly(A) signal. The bottom construct is the donor construct, comprising from 5′ to 3′: a phosphoglycerate kinase (PGK) promoter, a 5′ target site duplications (TSD) sequence, a 5′ terminal repeat (5′TR) sequence, a sequence encoding puromycin and enhanced GFP fusion (Puro-eGFP), a 3′ terminal repeat (3′TR) sequence, a 3′ target site duplications (TSD) sequence, and a poly(A) signal. In the transposition assay, the donor plasmid is co-transfected with the helper or control plasmids into cultured mammalian cells (e.g. human 293T. HeLa, or Hct116 cells), and the number of cell clones that are resistant to the puromycin due to chromosomal integration and expression of the puromycin resistance gene serves as an indicator of the efficiency of gene transfer, as demonstrated by methylene blue staining. For suspension cells, such as K562 and primary T cells, transposition activity can be assessed based on GFP reporter positive cells after electroporation.  FIGS.  4 A- 6 B , show the transposition efficiencies of the evaluated TEs as compared to controls based on colony counting of methylene blue staining results of the transposition assay. Transposition efficiency in such assays is also referred to as “transfer efficiency.” 
     In some embodiments, the transfer efficiency of the engineered transposable element is at least about any one of 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, or higher. In some embodiments, the transfer efficiency of the engineered transposable element is determined in a human cell, such as human 293 T, HeLa, Hct116, K562, or primary T cells. 
     In some embodiments, the transposition activity of the engineered transposable element is higher than that of a piggyBac (PB) transposon, a Sleeping Beauty (SB) transposon, and/or a TcBuster (TB) transposon. In some embodiments, the transposition activity of the engineered transposable element is higher than that of a piggyBac (PB) transposon, for example, by about any one of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 150%, 2×, 3×, 5×, 10× or more as determined by a reporter-based transposition assay (e.g., as described in Example 2) in a mammalian cell. In some embodiments, the transposition activity of the engineered transposable element is higher than that of a Sleeping Beauty (SB) transposon, for example, by about any one of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 150%, 2×, 3×, 5×, 10× or more as determined by a reporter-based transposition assay (e.g., as described in Example 2) in a mammalian cell. In some embodiments, the transposition activity of the engineered transposable element is higher than that of a TcBuster (TB) transposon, for example, by at least about any one of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 150%, 2×, 3×, 5×, 10× or more as determined by a reporter-based transposition assay (e.g., as described in Example 2) in a mammalian cell. In some embodiments, the transposition activity of the engineered transposable element is higher than that of a PB transposon and that of a SB transposon. In some embodiments, the transposition activity of the engineered transposable element is higher than that of a PB transposon and that of a TB transposon. In some embodiments, the transposition activity of the engineered transposable element is higher than that of a TB transposon and that of a SB transposon. In some embodiments, the transposition activity of the engineered transposable element is higher than that of a PB transposon, that of a SB transposon and that of a TB transposon. 
     In some embodiments, the transposition activity of the engineered transposable element is assessed in a mammalian cell. In some embodiments, the mammalian cell is a HeLa cell. In some embodiments, the mammalian cell is a human embryonic kidney 293T (293T). In some embodiments, the mammalian cell is a K562 cell. In some embodiments, the mammalian cell is a Hct116 cell. In some embodiments, the mammalian cell is a human T cell, such as primary T cell from a donor. In some embodiments, the engineered transposable element has higher transposition activity in a 293T cell than in a HeLa cell, such as at least about any one of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 150%, 2×, 3×, 5×, 10× or more transposition activity in a 293T cell than in a HeLa cell. 
     Cells 
     The engineered transposable elements described herein have transposition activity in a variety of cells, and can be used to insert heterologous nucleic acid into a target nucleic acid in any suitable cell. 
     In some embodiments, the cell is an isolated cell. In some embodiments, the cell is in cell culture. In some embodiments, the cell is ex vivo. In some embodiments, the cell is obtained from a living organism, and maintained in a cell culture. In some embodiments, the cell is a single-cellular organism. Cells may be classified into different types based on their sources, tissues of origin, morphologies, functions, histological markers, expression profiles, or the like. 
     In some embodiments, the cell is a prokaryotic cell. In some embodiments, the cell is a bacterial cell or derived from a bacterial cell. In some embodiments, the cell is an archaeal cell or derived from an archaeal cell. In some embodiments, the cell is a eukaryotic cell. In some embodiments, the cell is a plant cell or derived from a plant cell. In some embodiments, the cell is a fungal cell or derived from a fungal cell. In some embodiments, the cell is an animal cell or derived from an animal cell. In some embodiments, the cell is an invertebrate cell or derived from an invertebrate cell. In some embodiments, the cell is a vertebrate cell or derived from a vertebrate cell. In some embodiments, the cell is a mammalian cell or derived from a mammalian cell. In some embodiments, the cell is a human cell. In some embodiments, the cell is a zebra fish cell. In some embodiments, the cell is a rodent cell. In some embodiments, the cell is synthetically made, sometimes termed an artificial cell. In some embodiments, the cell is related to an animal species from which the 5′TR, 3′TR and/or the transposase are derived from. In some embodiments, the cell is not related to an animal species from which the 5′TR, 3′TR and/or the transposase are derived from. 
     In some embodiments, the cell is a bacterium, a yeast cell, a fungal cell, an algal cell, a plant cell, or an animal cell. In some embodiments, the cell is a cell isolated from natural sources, such as a tissue biopsy. In some embodiments, the cell is a cell isolated from an in vitro cultured cell line. In some embodiments, the cell is a genetically engineered cell. In some embodiments, the cell is a seed cell that undergoes proliferation, differentiation, or both in the core. 
     In some embodiments, the cell is an animal cell from an organism selected from the group consisting of cattle, sheep, goat, horse, pig, deer, chicken, duck, goose, rabbit, and fish. 
     In some embodiments, the cell is a plant cell from an organism selected from the group consisting of maize, wheat, barley, oat, rice, soybean, oil palm, safflower, sesame, tobacco, flax, cotton, sunflower, pearl millet, foxtail millet, sorghum, canola, cannabis, a vegetable crop, a forage crop, an industrial crop, a woody crop, and a biomass crop. 
     In some embodiments, the cell is a mammalian cell, including cells from humans, domestic and farm animals, and zoo, sports, or pet animals, such as dogs, horses, cats, cows, etc. In some embodiments, the cell is a human cell. In some embodiments, the human cell is a human embryonic kidney 293T (HEK293T or 293T) cell or a HeLa cell. 
     In some embodiments, the cell is derived from a primary cell. For example, cultures of primary cells can be passaged 0 times, 1 time, 2 times, 4 times, 5 times, 10 times, 15 times or more. In some embodiments, the primary cells are harvest from an individual by any known method. For example, leukocytes may be harvested by apheresis, leukocytapheresis, density gradient separation, etc. Cells from tissues such as skin, muscle, bone marrow, spleen, liver, pancreas, lung, intestine, stomach, etc. can be harvested by biopsy. An appropriate solution may be used for dispersion or suspension of the harvested cells. Such solution can generally be a balanced salt solution, (e.g. normal saline, phosphate-buffered saline (PBS), Hank&#39;s balanced salt solution, etc.), conveniently supplemented with fetal calf serum or other naturally occurring factors, in conjunction with an acceptable buffer at low concentration. Buffers can include HEPES, phosphate buffers, lactate buffers, etc. Cells may be used immediately, or they may be stored (e.g., by freezing). Frozen cells can be thawed and can be capable of being reused. Cells can be frozen in a DMSO, serum, medium buffer (e.g., 10% DMSO, 50% serum, 40% buffered medium), and/or some other such common solution used to preserve cells at freezing temperatures. 
     In some embodiments, the cell is derived from a cell line. A wide variety of cell lines are known in the art. Examples of cell lines include, but are not limited to, 293T, MF7, K562, HeLa, and transgenic varieties thereof. Cell lines are available from a variety of sources known to those with skill in the art (see, e.g., the American Type Culture Collection (ATCC) (Manassas, Va.)). 
     In some embodiments, the cell comprises an adherent cell. In some embodiments, the cell comprises a differentiated adherent cell. In some embodiments, the cell comprises an undifferentiated adherent cell. In some embodiments, the cell comprises a pluripotent stem cell. In some embodiments, the cell comprises a non-adherent cell. 
     In some embodiments, the cell is derived from an epithelial, muscular, nervous, or connective tissue, or any combination thereof. In some embodiments, the cell is derived from a tissue selected from the group consisting of liver, gastrointestinal, pancreatic, kidney, lung, tracheal, vascular, skeletal muscle, cardiac, skin, smooth muscle, connective tissue, corneal, genitourinary, breast, reproductive, endothelial, epithelial, fibroblast, neural, Schwann, adipose, bone, bone marrow, cartilage, pericytes, mesothelial, endocrine, stromal, lymph, blood, endoderm, ectoderm, mesoderm and combinations thereof. In some embodiments, the cell is derived from a tissue selected from the group consisting of connective tissue (for example, loose connective tissue, dense connective tissue, elastic tissue, reticular connective tissue and adipose tissue), muscle tissue (for example, skeletal muscle, smooth muscle and cardiac muscle), urogenital tissue, gastrointestinal tissue, lung tissue, bone tissue, nerve tissue and epithelial tissue (for example, a single layer of epithelial and stratified epithelium), endoderm-derived tissue, mesoderm-derived tissue and ectoderm-derived tissue, or any combination thereof. In some embodiments, the cell is derived from a tumor. 
     In some embodiments, the cell is selected from the group consisting of liver cell, gastrointestinal cell, pancreatic cell, kidney cell, lung cell, tracheal cell, vascular cell, skeletal muscle cell, cardiac cell, skin cell, smooth muscle cell, connective tissue cell, corneal cell, genitourinary cell, breast cell, reproductive cell, endothelial cell, epithelial cell, fibroblast, neural cell, Schwann cell, adipose cell, bone cell, bone marrow cell, cartilage cell, pericyte, mesothelial cell, cell derived from endocrine tissue, stromal cell, stem cell, progenitor cell, lymph cell, blood cell, endoderm-derived cell, ectoderm-derived cell, mesoderm-derived cell, undifferentiated cell (such as stem cell, or progenitor cell), tumor cell, iPS cell, and combinations thereof. 
     In some embodiments, the cell is an immune cell, such as T cells, B cells, Natural killer (NK) cells, dendritic cells (DCs) and macrophages. In some embodiments, the cell is a human T cell obtained from a patient or a donor. In some embodiments, the cell is an immune cell selected from the group consisting of a cytotoxic T cell, a helper T cell, a natural killer (NK) T cell, an iNK-T cell, an NK-T like cell, a αβT cell, a γδT cell, a tumor-infiltrating T cell and a dendritic cell (DC)-activated T cell. In some embodiments, the cell is an immune cell modified using the engineered transposable element or gene transfer system of the present application. In some embodiments, the modified immune cell is a CAR-T cell. In some embodiments, the modified immune cell is a TCR-T cell. 
     In some embodiments, the cell of the present application is a mammalian cell. In some embodiments, the mammalian cell is a human HKT293 cell or HeLa cell. In some further embodiments, the transposition activity of the transposable element is higher in 293T cells than in HeLa cells. In some embodiments, the mammalian the mammalian cell is selected from the group consisting of an immune cell, a hepatic cell, a tumor cell, a stem cell, a zygote, a muscle cell, and a skin cell. 
     In some embodiments, the cell is a stem cell or progenitor cell. Cells can include stem cells (e.g., adult stem cells, embryonic stem cells, iPS cells) and progenitor cells (e.g., cardiac progenitor cells, neural progenitor cells, etc.). Cells can include mammalian stem cells and progenitor cells, including rodent stem cells, rodent progenitor cells, human stem cells, human progenitor cells, etc. 
     In some embodiments, the cell is a diseased cell. A diseased cell can have altered metabolic, gene expression, and/or morphologic features. A diseased cell can be a cancer cell, a diabetic cell, and an apoptotic cell. A diseased cell can be a cell from a diseased subject. 
     In some embodiments, the cell of the present application belongs to a target cell type that is useful in gene therapy. Illustrative target cell types include hematopoietic stem cells, hematopoietic progenitor cells, myeloid progenitors, lymphoid progenitors, thrombopoietic progenitors, erythroid progenitors, granulopoietic progenitors, monocytopoictic progenitors, megakaryoblasts, promegakaryocytes, megakaryocytes, thrombocytes/platelets, proerythroblasts, basophilic erythroblasts, polychromatic erythroblasts, orthochromatic erythroblasts, polychromatic erythrocytes, erythrocytes (red blood cells or RBCs), basophilic promyelocytes, basophilic myelocytes, basophilic metamyelocytes, basophils, neutrophilic promyelocytes, neutrophilic myelocytes, neutrophilic metamyelocytes, neutrophils, eosinophilic promyelocytes, eosinophilic myelocytes, macrophages, dendritic cells, lymphoblasts, prolymphocytes, natural killer (NK)-cells, small lymphocytes, T-lymphocytes, B-lymphocytes, plasma cells, and lymphoid dendritic cells. In preferred embodiments, the target cell type is one or more erythroid cells, e.g., proerythroblast, basophilic erythroblast, polychromatic erythroblast, orthochromatic erythroblast, polychromatic erythrocyte, and erythrocyte (RBC). 
     Vectors 
     In some embodiments, the engineered transposable element and/or the nucleic acid sequence encoding the transposase is present in one or more vectors. 
     Various suitable vectors may be used in the present application. In some embodiments, the vector is a plasmid vector, a cosmid vector, an artificial chromosome (for example a bacterial artificial chromosome, a yeast artificial chromosome or a mammalian artificial chromosome), a viral vector such as a bacteriophage, baculovirus, retrovirus, lentivirus, adenovirus, Vaccinia virus, semliki forest virus or adeno-associated virus (AAV) vector, all of which are well known and can be purchased from commercial sources. In general, a suitable vector contains an origin of replication functional in at least one organism, a promoter sequence, convenient restriction endonuclease sites, and one or more selectable markers. 
     In some embodiments, the vector is a plasmid. In some embodiments, the plasmid can be transformed into bacteria to store or to amplify, and can be transfected into a mammalian cell. 
     Methods of introducing vectors into a mammalian cell are known in the art. The vectors can be transferred into a host cell by physical, chemical, and/or biological methods. It is contemplated that various vector types and vector delivery methods may be used, either alone or in combination, for the present application. 
     Physical methods for introducing the vector into a host cell include calcium phosphate precipitation, lipofection, particle bombardment, microinjection, electroporation, and the like. Methods for producing cells comprising vectors and/or exogenous nucleic acids are well-known in the art. See, for example, Sambrook et al. (2001) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York. In some embodiments, the vector is introduced into the cell by electroporation. 
     Biological methods for introducing the heterologous nucleic acid into a host cell include the use of DNA and RNA vectors. Viral vectors have become the most widely used method for inserting genes into mammalian, e.g., human cells. 
     Chemical means for introducing the vector into a host cell include colloidal dispersion systems, such as macromolecule complexes, nanocapsules, microspheres, beads, and lipid-based systems including oil-in-water emulsions, micelles, mixed micelles, and liposomes. An exemplary colloidal system for use as a delivery vehicle in vitro is a liposome (e.g., an artificial membrane vesicle). 
     In some embodiments, the vector is a viral vector. Examples of viral vectors include, but are not limited to, adenoviral vectors, adeno-associated virus (AAV) vectors, lentiviral vector, retroviral vectors, vaccinia vector, herpes simplex viral vector, and derivatives thereof. Viral vector technology is well known in the art and is described, for example, in Sambrook et al. (2001, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York), and in other virology and molecular biology manuals. In some embodiments, delivery of a transposable element using viral vectors may be useful for gene therapy in combining the high efficiency of gene delivery by the viral vectors and the stability of gene expression enabled by the transposable element. Reference may be made to, for example, Yant, Stephen R., et al. “Transposition from a gutless adeno-transposon vector stabilizes transgene expression in vivo.” Nature biotechnology 20.10 (2002): 999-1005. 
     III. Gene Transfer Systems 
     Another aspect of the present application provides a gene transfer system comprising: 1) an engineered transposable element (such as any one of the transposable elements describe herein); and 2) a transposase, or a nucleic acid encoding a transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element, comprising from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; and 2) a transposase. In some embodiments, the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell (e.g., mammalian cell or plant cell). In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element, comprising from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; and 2) a nucleic acid (e.g., DNA or RNA) encoding a transposase. In some embodiments, the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell (e.g., mammalian cell or plant cell). In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element, comprising from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR); and 2) a transposase comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell (e.g., mammalian cell or plant cell). In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element, comprising from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR); and 2) a nucleic acid (e.g., DNA or RNA) encoding a transposase comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell (e.g., mammalian cell or plant cell). In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element; and 2) a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell (e.g., mammalian cell or plant cell). In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a gene transfer system comprising a gene transfer system comprising: 1) an engineered transposable element; and 2) a nucleic acid (e.g., DNA or RNA) encoding a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell (e.g., mammalian cell or plant cell). In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 3, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 29, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 55, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 8, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 34, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 60, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 11, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 37, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 63, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 12, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 38, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 64, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 13, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 39, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 65, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 16, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 42, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 68, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 22, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 48, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 74, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 23, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 49, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 75, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 79, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 91, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 103, or a nucleic acid encoding the transposase. 
     In some embodiments, there is provided a gene transfer system comprising: 1) an engineered transposable element comprising, from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises the nucleic acid sequence of SEQ ID NO: 82, a variant thereof, or a fragment thereof, wherein the 3′TR comprises the nucleic acid sequence of SEQ ID NO: 94, a variant thereof, or a fragment thereof; and 2) a transposase comprising the amino acid sequence of SEQ ID NO: 106, or a nucleic acid encoding the transposase. 
     The gene transfer systems described herein comprises a transposase. The transposase may be present as a polypeptide. Alternatively, the transposase is present as a polynucleotide that includes a coding sequence encoding a transposase. The polynucleotide can be RNA, for instance an mRNA encoding the transposase, or DNA, for instance a coding sequence encoding the transposase. When the transposase is present as a coding sequence encoding the transposase, in some embodiments, the coding sequence may be present in the same vector that includes the transposable element, i.e., in cis. In some embodiments, the gene transfer system comprises a first vector comprising the engineered transposable element, and a second vector comprising the transposase coding sequence, i.e., in trans. 
     In some embodiments, the gene transfer system comprises: 1) a vector comprising an engineered transposable element (such as any one of the engineered transposable elements described herein); and 2) a transposase. 
     In some embodiments, the gene transfer system comprises: 1) an engineered transposable element (such as any one of the engineered transposable elements described herein); and 2) a nucleic acid encoding a transposase, wherein the nucleic acid is DNA. In some embodiments, the engineered transposable element and the nucleic acid are present in a single vector. In some embodiments, the engineered transposable element and the nucleic acid are present in separate vectors. 
     In some embodiments, the gene transfer system comprises: 1) an engineered transposable element (such as any one of the engineered transposable elements described herein); and 2) a nucleic acid encoding a transposase, wherein the nucleic acid is RNA. In some embodiments, the engineered transposable element and the nucleic acid are present in a single vector. In some embodiments, the engineered transposable element and the nucleic acid are present in separate vectors. 
     There are many potential and suitable combinations of intracellular delivery methods for the engineered transposable element and the transposase or nucleic acid encoding the transposase in the gene transfer systems described herein. The transposase may be delivered as a DNA, RNA, or protein. The engineered transposable element and the transposase may be delivered together or separately. For example, both the transposon and the transposase gene can be contained together in the same recombinant viral genome; a single infection delivers both parts of the system such that expression of the transposase directs cleavage of the transposon from the recombinant viral genome for subsequent integration into a cellular chromosome. In another example, the transposase and the transposable element can be delivered separately by a combination of viruses and/or non-viral systems such as lipid-containing reagents. In these cases, either the transposable element and/or the transposase gene can be delivered by a recombinant virus. In some embodiments, the transposase directs liberation of the transposon from its donor DNA for integration into a target location. 
     The transposase can be provided to the cell as protein or as nucleic acid encoding the transposase protein. Nucleic acid encoding the transposase protein can take the form of DNA or RNA. The protein can be introduced into the cell alone, or in a vector, such as a plasmid or a viral vector. Further, the nucleic acid encoding the transposase protein can be stably or transiently incorporated into the genome of the cell to facilitate temporary or prolonged expression of the transposase protein in the cell. Further, promoters or other expression control regions can be operably linked with the nucleic acid encoding the transposase protein to regulate expression of the protein in a quantitative or in a tissue-specific manner. In some embodiments, the transposase protein contains a DNA-binding domain, a catalytic domain (having transposase activity), and/or a nuclear localization signal (NLS). 
     As such, various methods and materials may be used in delivering the gene transfer system of the present application to a cell. 
     By way of example, one approach is to use plasmid vectors to deliver the gene transfer system. By way of example, the system may consist of two plasmids, one helper plasmid carrying the transposase expression cassette and one donor plasmid carrying the transposable element. After transfection, both plasmids find way to the nucleus, allowing production of transposase-encoding RNA from the helper plasmid and subsequent excision of the transposable element from the donor plasmid facilitated by transposase subunits imported into the nucleus. This approach can be further refined by placing both the transposase gene and the transposable element on a single plasmid, originally referred to as helper-independent transposable element-transposase vectors. Alternatively, transfected in vitro-transcribed mRNA may serve as a rich source of transposase, eliminating the risk of creating cells with prolonged expression of the transposase. 
     Accordingly, in some embodiments, the transposable element is present in a first vector (a donor vector), and a nucleic acid encoding a transposase is present in a second vector (a helper vector). In some embodiments, the first vector and the second vector are used to co-transfect a cell for transposition. 
     Another approach is to use viral vectors for the delivery of the gene transfer system. Although viral vectors could have immunogenic or carcinogenic issues, the high delivery efficiencies may be desirable in certain applications. The components of the gene transfer system may be carried and delivered by viral capsids, providing otherwise episomal vectors—like adenoviral or herpes simplex virus-based vectors—the ability to integrate genes and establish long-term transgene expression. The viral coat may provide vector stability, tissue-specific transposable element delivery and transport across the cellular membrane, while the transposable element facilitates viral vector integration according to the characteristic integration profile of the transposable element. Methods and techniques of viral vector-based delivery are known in the art. For example, viral vector-based transfer of the Sleeping Beauty transposon system was first demonstrated in mouse liver with adenoviral vectors, and recent studies have demonstrated the applicability of this approach in larger animals. Adeno-associated viral vectors have also been adapted as carriers of the Sleeping Beauty system. See. e.g., Yant, Stephen R., et al. “Transposition from a gutless adeno-transposon vector stabilizes transgene expression in vivo.”  Nature biotechnology  20.10 (2002): 999-1005, Hausl, Martin A., et al. “Hyperactive sleeping beauty transposase enables persistent phenotypic correction in mice and a canine model for hemophilia B.”  Molecular Therapy  18.11 (2010): 1896-1906, and Zhang, Wenli, et al. “Hybrid adeno-associated viral vectors utilizing transposase-mediated somatic integration for stable transgene expression in human cells.”  PloS one  8.10 (2013). 
     As discussed above, the gene transfer system of the present application may be delivered into a host cell by physical, chemical, biological methods, or a combination thereof. It is contemplated that various delivery vectors and methods may be used for the present application, either alone or in combination, in order to achieve desirable results for certain applications. Those skilled in the art are thereby enabled to best utilize the various delivery methods and techniques with various modifications as are suited to the particular use contemplated, for example, gene therapy. For gene therapy to be practical, one should achieve stable integration of a therapeutic transgene in the genome of an afflicted tissue to provide a long-term and cost-effective treatment. For example, to achieve a high-efficiency and low-immunogenic gene transfer into patients, one may combine the use of synthetic compounds and plasmids for delivering DNA into a cell. Liposomes and other nanoparticles may be sufficient for this task. For instance, two plasmids can be delivered to the patient: one that provides expression of the transposase (a helper plasmid), and another that provides the transposable element containing a therapeutic transgene (the donor plasmid). These DNAs can be complexed with liposomes and administered via parenteral injection. Upon entering a cell, the transposase may bind to the transposable element in the donor plasmid, excise it, and then integrate it into the genome. Such insertions will be stable and permanent. The helper and donor plasmids may eventually be lost by cellular- and host-defense mechanisms, but any genome-integrated transposable element, containing the therapeutic transgene, will be stable and permanent modifications. The transient nature of these plasmids also curtails excessive transposition, and thus minimizes the risk of carcinogenesis. 
     Further details and exemplary transposable element delivery methods and techniques may be found in, e.g., Skipper, Kristian Alsbjerg, et al. “DNA transposon-based gene vehicles-scenes from an evolutionary drive.”  Journal of biomedical science  20.1 (2013): 92. 
     IV. Methods 
     The present application further provides methods of inserting a heterologous nucleic acid into a target nucleic acid, comprising: contacting the target nucleic acid with an engineered transposable element comprising the heterologous nucleic acid according to any one of the engineered transposable elements described herein or a gene transfer system comprising the heterologous nucleic acid according to any one of the gene transfer systems described herein. The method may be carried out in vitro, or in a cell. 
     In Vivo Methods 
     In some embodiments, there is provided a method of inserting a heterologous nucleic acid into a target nucleic acid in vino, comprising contacting the target nucleic acid with an engineered transposable element comprising: 1) an engineered transposable element; and 2) a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the target nucleic acid is a circular DNA. In some embodiments, the target nucleic acid is a linear DNA. In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     The methods described herein are useful for in vitro transposition, including in a cell-free system. See, for example, Goryshin, Igor Yu, and William S. Reznikoff. “Tn5 in vitro transposition.”  Journal of Biological Chemistry  273.13 (1998): 7367-7374. Transposable elements exhibiting in vitro transposition activity may be useful for next-generation sequence (NGS) library construction, including, for example, tagmentation methods. 
     In some embodiments, there is provided a method of preparing a plurality of barcoded nucleic acids from a target nucleic acid, comprising contacting the target nucleic acid with an engineered transposable element comprising: 1) an engineered transposable element; and 2) a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid comprising a barcode sequence, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof, thereby provided a plurality of barcoded nucleic acids. In some embodiments, the target nucleic acid is genomic DNA. In some embodiments, the target nucleic acid is cDNA. In some embodiments, the target nucleic acid is amplified DNA. In some embodiments, the heterologous nucleic acid further comprises a primer sequence. In some embodiments, the method further comprises amplifying the plurality of barcoded nucleic acids to provide a nucleic acid sequencing library. In some embodiments, the method further comprises sequencing the nucleic acid sequencing library. In some embodiments, the method comprises contacting the target nucleic acid with a plurality of engineered transposable element, wherein each engineered transposable element comprises a unique barcode sequence. In some embodiments, the nucleic acid sequencing libraries prepared using the in vitro methods described herein preserve contiguity information in a target nucleic acid sequence. In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, tagmentation methods are provided using any one of the transposases and/or TR sequences described herein. Tagmentation methods using Tn5 transposon are known in the art, for example, in U.S. Pat. No. 9,080,211B2, which is incorporated herein by reference in its entirety. The tagmentation methods described herein uses transposome complex compositions. 
     In some embodiments, there is provided a transposome complex composition, comprising: a transposase comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof and a heterologous nucleic acid comprising one or two TR sequences and a tag sequence. In some embodiments, transposome complex comprises a single heterologous nucleic acid that forms a hairpin. In some embodiments, the hairpin comprises a cleavable site. 
     In some embodiments, the transposome complex comprises two of the transposases bound to two heterologous nucleic acids. In some embodiments, there is provided a transposome complex composition, comprising: a first transposase bound to a first heterologous nucleic acid comprising a 5′TR sequence and a first tag sequence, and a second transposase bound to a second heterologous nucleic acid comprising a 3′TR sequence and a second tag sequence. In some embodiments, the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, and wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof. In some embodiments, the first tag sequence is different from the second tag sequence. In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a method for preparing a library of nucleic acid (e.g., DNA) fragments having first and second tag sequences for a target nucleic acid, comprising contacting the target nucleic acid with a plurality of transposome complexes comprising: (1) a first transposome complex comprising a first transposase and a first heterologous nucleic acid comprising a TR sequence and a first tag sequence; and (2) a second transposome complex comprising a second transposase and a second heterologous nucleic acid comprising a TR sequence and a second tag sequence, wherein the first tag sequence is different from the second tag sequence, and wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof; wherein the first heterologous nucleic acid and the second heterologous nucleic acid are inserted into the target nucleic acid and the target nucleic acid is fragmented into a multitude of nucleic acid fragments comprising one of the first or the second nucleic acids attached to each 5′ end of the nucleic acid fragments; thereby providing the library of nucleic acid fragments. In some embodiments, the transposome complex of (1) comprises two of the first heterologous nucleic acids, and the transposome complex of (2) comprises two of the second heterologous nucleic acids. In some embodiments, the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, and wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof. In some embodiments, the method further comprises amplifying the nucleic acid fragments. In some embodiments, the method further comprises sequencing the nucleic acid fragments or amplicons thereof. In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, the engineered transposable element or transposome complex inserts into the target nucleic acids in a random fashion, i.e., without bias for specific sequence motifs. In some embodiments, the engineered transposable element or transposome complex inserts into the target nucleic acids more randomly than a PB, SB, or TB transposon. 
     In some embodiments, the engineered transposable element or transposome complex preferentially inserts into sterically free regions of a target nucleic acid, such as open chromatin region, regions free from binding by nucleosome or other DNA binding proteins. The in vitro methods described herein can thus be used to prepare nucleic acid sequencing libraries for assays to study epigenomics (e.g., chromatin remodeling or DNA methylation), including, but not limited to, Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), Cleavage Under Targets and Tagmentation (CUT&amp;TAG), Assay for Transposase-Accessible Chromatin and DNA methylation (ATAC-Me), and Transposase-mediated analysis of chromatin looping (Trac-looping). ATAC-seq, CUT&amp;TAG, ATAC-Me and Trac-looping assays using Tn5 transposon have been described, for example, in Buenrostro, Jason D., et al. “Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position.”  Nature methods  (2013) 10(2): 1213; Kaya-Okur H S, et al., “CUT&amp;Tag for efficient epigenomic profiling of small samples and single cells.”  Nature Communications  (2019), 10(1):1-10; Barnett K R et al. “ATAC-Me Captures Prolonged DNA Methylation of Dynamic Chromatin Accessibility Loci during Cell Fate Transitions.”  Molecular Cell,  2020; and Lai B. et al. “Trac-looping measures genome structure and chromatin accessibility,”  Nature methods,  2018, 15(9): 741, which are incorporated herein by reference in their entirety. In some embodiments, the sequencing library preparation is for use in Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq). 
     In some embodiments, the engineered transposable element or transposome complex can be used to tag regions of a target nucleic acid (e.g., a genomic DNA) that are spatially in proximity. Two regions that are spatially in proximity with each other may contain the same pair of tag sequences at the ends. The in vitro methods described herein can thus be used to prepare fluorescently labeled probes useful for in situ hybridization to chromatin interaction boundaries in genomic DNA, for example, in a transposase-based fluorescence in situ hybridization (FISH). Tn5-based FISH methods have been described, for example, in Zhang X. et al. “Imaging chromatin interactions at sub-kilobase resolution via Tn5-FISH,” bioRxiv, 2019:601690, which is incorporated by reference in its entirety. 
     Methods in Cell 
     In some embodiments, there is provided a method of inserting a heterologous nucleic acid into a target nucleic acid in a cell, comprising contacting the target nucleic acid with an engineered transposable element comprising: 1) an engineered transposable element; and 2) a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the target nucleic acid is genomic DNA. In some embodiments, the target nucleic acid is extrachromosomal DNA. In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a method of inserting a heterologous nucleic acid into a target nucleic acid in a mammalian cell, comprising contacting the target nucleic acid with an engineered transposable element comprising: 1) an engineered transposable element; and 2) a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the mammalian cell is a human cell. In some embodiments, the mammalian cell is an animal cell, such as a rodent cell. In some embodiments, the mammalian cell is an immune cell, such as T cell. In some embodiments, the method is carried out ex vivo. In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a method of inserting a heterologous nucleic acid into a target nucleic acid in a plant cell, comprising contacting the target nucleic acid with an engineered transposable element comprising: 1) an engineered transposable element; and 2) a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof. In some embodiments, the plant cell is a cell of a crop plant. In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     The transposable elements or gene transfer systems described herein may be introduced into one or more cells using any of a variety of techniques known in the art such as, but not limited to, microinjection, combining the nucleic acid fragment with lipid vesicles, such as cationic lipid vesicles, particle bombardment, electroporation, DNA condensing reagents (e.g., calcium phosphate, polylysine or polyethyleneimine) or incorporating the nucleic acid fragment into a viral vector and contacting the viral vector with the cell. Where a viral vector is used, the viral vector can include any of a variety of viral vectors known in the art including viral vectors selected from the group consisting of a retroviral vector, an adenovirus vector or an adeno-associated viral (AAV) vector. 
     It is contemplated that the heterologous nucleic acid may contain multiple operons or the heterologous nucleic acid may encode more than one biological products. In some embodiments, the heterologous nucleic acid encodes a genetic circuit. An exemplary genetic circuit is a collection of parts that undergo transcription and/or translation to produce mRNA or proteins, respectively (each an “output” of the part). The part output can interact with other parts (for example to regulate transcription or translation) or can interact with other molecules in the cell (e.g., small molecules, DNA, RNA or proteins that are present in the cellular environment). For example, a circuit can be a metabolic pathway or a genetic cascade, which can be naturally occurring or non-naturally occurring, artificially engineered. Each part in the circuit can include a set of components or genetic modules, e.g., a promoter, ribosome binding site (RBS), coding sequence (CDS) and/or terminator. These components may be interconnected or assembled in different ways to implement different parts, and the resultant parts may be combined in different ways to create different circuits or pathways. In addition to these parts, the circuit may contain additional molecular species that are present in a cell or in the cell&#39;s environment that the components interact with. 
     Accordingly, in some embodiments, there is provided a method of inserting a heterologous nucleic acid into a target nucleic acid in a cell, comprising: contacting the cell with an engineered transposable element comprising: 1) an engineered transposable element; and 2) a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof, wherein the heterologous nucleic acid encodes a genetic circuit. In some embodiments, the target nucleic acid is genomic DNA. In some embodiments, the target nucleic acid is an extrachromosomal DNA. In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     Genetic circuits can be useful for gene therapy. Methods and techniques of designing and using genetic circuits are known in the art. Further reference may be made to, for example, Brophy, Jennifer A N, and Christopher A. Voigt. “Principles of genetic circuit design.”  Nature methods  11.5 (2014): 508. 
     The methods are applicable for any suitable cell type. In some embodiments, the cell is a bacterium, a yeast cell, a fungal cell, an algal cell, a plant cell, or an animal cell. In some embodiments, the cell is a cell isolated from natural sources, such as a tissue biopsy. In some embodiments, the cell is a cell isolated from an in vitro cultured cell line. In some embodiments, the cell is a genetically engineered cell. In some embodiments, the cell is a seed cell that undergoes proliferation, differentiation, or both in the core. 
     In some embodiments, the cell is an animal cell from an organism selected from the group consisting of cattle, sheep, goat, horse, pig, deer, chicken, duck, goose, rabbit, and fish. 
     In some embodiments, the cell is a plant cell from an organism selected from the group consisting of maize, wheat, barley, oat, rice, soybean, oil palm, safflower, sesame, tobacco, flax, cotton, sunflower, pearl millet, foxtail millet, sorghum, canola, cannabis, a vegetable crop, a forage crop, an industrial crop, a woody crop, and a biomass crop. 
     In some embodiments, the cell is a mammalian cell. In some embodiments, the cell is a human cell. In some embodiments, the human cell is a human embryonic kidney 293T (HEK293T or 293T) cell or a HeLa cell. In some embodiments, the mammalian the mammalian cell is selected from the group consisting of an immune cell, a hepatic cell, a tumor cell, a stem cell, a zygote, a muscle cell, and a skin cell. 
     In some embodiments, the cell is an immune cell selected from the group consisting of a cytotoxic T cell, a helper T cell, a natural killer (NK) T cell, an iNK-T cell, an NK-T like cell, a γδT cell, a tumor-infiltrating T cell and a dendritic cell (DC)-activated T cell. In some embodiments, the method produces a modified immune cell, such as a CAR-T cell or a TCR-T cell. 
     In some embodiments, the heterologous nucleic acid is inserted into the genome of the cell. In some further embodiments, insertion of the heterologous nucleic acid inactivates a gene of the cell. In some embodiments, the heterologous nucleic acid encodes a protein or an RNAi molecule. 
     In addition, the heterologous nucleic acid may encode an RNA molecule that is useful in genome editing. Examples of such RNA molecules include, but are not limited to, CRISPR RNA (crRNA), trans-activating crRNA (tracrRNA), guide RNA (gRNA), and single guide RNA (sgRNA). 
     In some embodiments, the heterologous nucleic acid encodes a biological product selected from the group consisting of a reporter protein, an antigen-specific receptor, a therapeutic protein, an antibiotic resistance protein, an RNAi molecule, a cytokine, a kinase, an antigen, an antigen-specific receptor, a cytokine receptor, and a suicide polypeptide. For example, the heterologous nucleic acid can encode a receptor specific to a tumor-associated antigen. A T-cell engineered via the method is capable of recognizing and specifically killing the tumor cells expressing the tumor-associated antigen. In another example, the heterologous nucleic acid encodes a hygromycin-resistance protein so that a hygromycin-resistance cell line can be established. Alternatively, the heterologous nucleic acid may not possess any biological function, and can be used to interrupt the function of another gene by inserting itself into an essential gene, thereby interrupting its function. 
     In some particular embodiments, the heterologous nucleic acid encodes a therapeutic protein that is useful for gene therapy. In some embodiments, the heterologous nucleic acid encodes a therapeutic antibody. In some embodiments, the heterologous nucleic acid encodes an engineered receptor, such as a chimeric antigen receptor (CAR), or an engineered TCR. 
     In some embodiments, the heterologous nucleic acid comprises one or more multiple cloning sites (MCSs) to facilitate insertion of a polynucleotide of interest (“cargo gene”). 
     In some embodiments, the method is carried out ex vivo. In some embodiments, the transduced or transfected cell (e.g., mammalian cell) is propagated ex vivo after introduction of the heterologous nucleic acid into the cell. In some embodiments, the transduced or transfected cell is cultured to propagate for at least about any of 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 10 days, 12 days, or 14 days. In some embodiments, the transduced or transfected cell is cultured for no more than about any of 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 10 days, 12 days, or 14 days. In some embodiments, the transduced or transfected cell is further evaluated or screened to select the engineered cell. 
     Reporter genes or selectable markers may be used for identifying potentially transfected cells and for evaluating the functionality of regulatory sequences. In general, a reporter gene is a gene that is not present in or expressed by the recipient organism or tissue and that encodes a polypeptide whose expression is manifested by some easily detectable property, e.g., enzymatic activity. Expression of the reporter gene is assayed at a suitable time after the DNA has been introduced into the recipient cells. Suitable reporter genes may include genes encoding luciferase, beta-galactosidase, chloramphenicol acetyl transferase, secreted alkaline phosphatase, or the green fluorescent protein gene (e.g., Ui-Tei et al. FEBS Letters 479: 79-82 (2000)). Suitable expression systems are well known and may be prepared using known techniques or obtained commercially. 
     Other methods to confirm the presence of the heterologous nucleic acid in the cell, include, for example, molecular biological assays well known to those of skill in the art, such as Southern and Northern blotting, RT-PCR and PCR; biochemical assays, such as detecting the presence or absence of a particular peptide, e.g., by immunological methods (such as ELISAs and Western blots). 
     The present application contemplates methods of generating isogenic lines of cells of mammalian cells for the study of genetic variations. The present application also contemplates genome modification of microbes, cells, plants, animals or synthetic organisms for the generation of biomedically, agriculturally, and industrially useful products. The methods may be used as a biological research tool, for understanding the genome, e.g. gene knockout or knock-in studies. 
     Also provided are cells modified by a heterologous nucleic acid using any one of the methods described herein, and organisms (such as animals, plants, or fungi) comprising or produced from such cells. 
     Target Nucleic Acid 
     The methods described herein are suitable for inserting a heterologous nucleic acid into a variety of target nucleic acids. In some embodiments, the target nucleic acid is a DNA. In some embodiments, the target nucleic acid is single-stranded. In some embodiments, the target nucleic acid is double-stranded. In some embodiments, the target nucleic acid comprises both single-stranded and double-stranded regions. In some embodiments, the target nucleic acid is linear. In some embodiments, the target nucleic acid is circular. In some embodiments, the target nucleic acid comprises one or more modified nucleotides, such as methylated nucleotides, damaged nucleotides, or nucleotides analogs. In some embodiments, the target nucleic acid is not modified. In some embodiments, the target nucleic acid is bound to one or more proteins, such as nucleosomes. 
     The target nucleic acid may be of any length, such as about at least any one of 100 bp, 200 bp, 500 bp, 1000 bp, 2000 bp, 5000 bp, 10 kb, 20 kb, 50 kb, 100 kb, 200 kb, 500 kb, 1 Mb, or longer. In some embodiments, the target nucleic acid is no more than about any one of 500 kb, 200 kb, 100 kb, 50 kb, 40 kb, 30 kb, 20 kb, 10 kb, 5 kb, 2 kb, 1 kb, 500 bp, 200 bp or less. In some embodiments, the target nucleic acid is about any one of 100 bp-500 bp, 500 bp-1 kb, 100 bp-1 kb, 1 kb-2 kb, 100 bp-5 kb, 100 bp-10 kb, 100 bp-20 kb, 1 kb-5 kb, 1 kb-10 kb, 1 kb-20 kb, 20 kb-100 kb, or 100 kb-1 Mb. The target nucleic acid may also comprise any sequence. In some embodiments, the target nucleic acid is enriched for particular sequences, which are hotspots for transposition by the engineered transposable element or gene delivery system described herein. In some embodiments, the target nucleic acid is AT-rich, such as having at least about any one of 40%, 45%, 50%, 55%, 60%, 65%, or higher AT content. In some embodiments, the target nucleic acid is not AT-rich. In some embodiments, the target nucleic acid is not enriched for particular hotspot sequences, as the engineered transposable element or gene delivery system described herein has no preference to insert the heterologous nucleic acid into a particular sequence or sequence motif. In some embodiments, the target nucleic acid has one or more secondary structures or higher-order structures. In some embodiments, the target nucleic acid is not in a condensed state, such as in a chromatin. 
     In some embodiments, the target nucleic acid is present in a cell. In some embodiments, the target nucleic acid is present in the nucleus of the cell. In some embodiments, the target nucleic acid is endogenous to the cell. In some embodiments, the target nucleic acid is a genomic DNA. In some embodiments, the target nucleic acid is a chromosomal DNA. In some embodiments, the target nucleic acid is a protein-coding gene or a functional region thereof, such as a coding region, or a regulatory element, such as a promoter, enhancer, a 5′ or 3′ untranslated region, etc. In some embodiments, the target nucleic acid is a non-coding gene, such as transposon, miRNA, tRNA, ribosomal RNA, ribozyme, or lincRNA. In some embodiments, the target nucleic acid is a plasmid. 
     In some embodiments, the target nucleic acid is exogenous to a cell. In some embodiments, the target nucleic acid is a viral nucleic acid, such as viral DNA. In some embodiments, the target nucleic acid is a horizontally transferred plasmid. In some embodiments, the target nucleic acid is integrated in the genome of the cell. In some embodiments, the target nucleic acid is not integrated in the genome of the cell. In some embodiments, the target nucleic acid is a plasmid in the cell. In some embodiments, the target nucleic acid is present in an extrachromosomal array. 
     In some embodiments, the target nucleic acid is an isolated nucleic acid, such as an isolated DNA. In some embodiments, the target nucleic acid is present in a cell-free environment. In some embodiments, the target nucleic acid is an isolated vector, such as a plasmid. In some embodiments, the target nucleic acid is an isolated linear DNA fragment. 
     V. Kits and Articles of Manufacture 
     The present application also provides kits and articles of manufacture comprising any one of the transposable elements, or gene transfer systems describe herein. In some embodiments, the kit comprises instructions for inserting a heterologous nucleic acid into a target nucleic acid (e.g., using any one of the methods described herein). In some embodiments, the kit is for inserting a heterologous nucleic acid into a target nucleic acid in vitro. In some embodiments, the kit is for inserting a heterologous nucleic acid into a target nucleic acid in a cell, such as mammalian cell or plant cell. The kits and articles of manufacture described herein can be used for modification of a target nucleic acid in vitro or ex vivo, in genetic research, and in gene therapy. 
     In some embodiments, there is provided a kit comprising an engineered transposable element, comprising from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof, and wherein the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell (e.g., mammalian cell or plant cell). In some embodiments, the heterologous nucleic acid comprises one or more multiple cloning sites (MCSs) to facilitate insertion of a polynucleotide of interest (“cargo gene”). In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, there is provided a kit comprising a gene transfer system comprising: 1) an engineered transposable element; and 2) a transposase, or a nucleic acid encoding a transposase, wherein the transposable element comprises from 5′ to 3′: a 5′ terminal repeat sequence (5′TR), a heterologous nucleic acid, and a 3′ terminal repeat sequence (3′TR), wherein the 5′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-26 and 79-90, a variant thereof, or a fragment thereof, wherein the 3′TR comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 27-52 and 91-102, a variant thereof, or a fragment thereof; wherein the transposase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 53-78 and 103-114, or a variant thereof, and wherein the transposable element exhibits transposition activity that allows the heterologous nucleic acid to be inserted into the DNA of a cell (e.g., mammalian cell or plant cell). In some embodiments, the heterologous nucleic acid comprises one or more multiple cloning sites (MCSs) to facilitate insertion of a polynucleotide of interest (“cargo gene”). In some embodiments, the engineered transposable element is derived from any one of the TEs of Table 2. In some embodiments, the engineered transposable element is derived from Tc1-8B_DR, Tc1-3_FR, Mariner2_AG, Tc1-1_Xt, or Tc1-1_PM. 
     In some embodiments, the kit comprises one or more reagents for use in any one of the methods described herein. Reagents may be provided in any suitable container. For example, the kit may provide one or more reaction or storage buffers. Reagents may be provided in a form that is usable in a particular assay, or in a form that requires addition of one or more other components before use (e.g. in concentrate or lyophilized form). A buffer can be any buffer, including but not limited to a sodium carbonate buffer, a sodium bicarbonate buffer, a borate buffer, a Tris buffer, a MOPS buffer, a HEPES buffer, and combinations thereof. In some embodiments, the kit comprises culturing media, buffers, reagents and the like to allow propagation or induction of a cell modified using an engineered transposable element or gene transfer system described herein. In some embodiments, the kit comprises buffers, reagents and the like for isolating and/or preparing modified target nucleic acids using an engineered transposable element or gene transfer system described herein. In some embodiments, the kit comprises primers and reagents for preparation of sequencing libraries using an engineered transposable element or gene transfer system described herein. 
     The kits are in suitable packaging. Suitable packaging includes, but is not limited to, vials, bottles, jars, flexible packaging (e.g., sealed Mylar or plastic bags), and the like. Kits may optionally provide additional components such as buffers and interpretative information. The present application thus also provides articles of manufacture, which include vials (such as sealed vials), bottles, jars, flexible packaging, and the like. 
     EXAMPLES 
     The examples below are intended to be purely exemplary of the invention and should therefore not be considered to limit the invention in any way. The following examples and detailed description are offered by way of illustration and not by way of limitation. 
     Example 1: Identification of Candidate Active Transposable Elements 
     This example describes the in silico identification of candidate active transposable elements (TEs, transposons) across species. There exists a large amount of transposons in various species. However, only a few number of transposons have been characterized that exhibit transposition activity in mammalian cells. Therefore, there is a need for a methodology to systematically identify candidate active transposons that could be useful as agents for genome engineering and gene therapy. This example concentrates on identification of transposons with terminal inverted repeats (TIRs, also known as terminal repeats-TRs), but the methodology can be used to identify any other types of transposons. 
     Materials and Methods 
     Repbase is the most commonly used transposon database, which holds a collection of 38,000 transposon sequences from a wide range of eukaryotic species (Bao, W., K. K. Kojima, and O. Kohany, Repbase Update, a database of repetitive elements in eukaryotic genomes. Mob DNA, 2015, 6: p. 11). These prototypic sequences are mostly consensus sequences, which are reconstructed for each transposon family to approximate the sequence of its ancestral active status. Thus, the consensus sequences can be used to experimentally reconstruct active transposons for transgenesis and gene therapy, such as Sleeping Beauty (Ivics, Z., et al., Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell, 1997, 91(4): p. 501-10). As an initial step of this project, all consensus sequences were downloaded from Repbase (version Repbase24.02) for candidate transposon screening. 
     Because the consensus sequences do not necessarily contain an intact transposase gene (especially for those ancient transposons that have highly degenerated) and may not function as an active transposon in other systems, such as mammalian cells, several parameters were used to evaluate the identified TE transposition activity in their original species, including important domains encoded by transposase, average sequence divergence between the consensus and family members, copy number, conserved terminal inverted repeats (TR) sequences, and conserved target site duplications (TSDs) flanking the TEs. To obtain the abovementioned information, genome sequences of 100 animals were downloaded from UCSC Genome Browser (Haeussler, M., et al., The UCSC Genome Browser database: 2019 update. Nucleic Acids Res, 2019, 47(D1): p. D853-D858). The genome sequences were masked for repeats using the consensus sequences from Repbase. An active transposon is defined such that: 1) the candidate transposon matches the consensus sequence from start to end; and 2) the length of the candidate transposon reaches 90% length of the consensus transposon to ensure no significant deletions within the transposon. 
     Results 
       FIG.  1    illustrates a flow chart of the bioinformatics pipeline, as well as the number of candidate active transposable elements at each stage of the pipeline. 
     In total, 26,853,019 copies of DNA transposons (mostly TIR transposons) were identified from the 100 animal genomes. A large number of the copies were fragments degenerated from the active transposons. The number of full-length DNA transposon copies was 1,895,466 in total, which were mapped to 1,577 consensus DNA transposons in Repbase. 
     These transposons were then examined to see if they contain a transposase gene. ORFfinder was used to detect open reading frames (ORFs), and the length cutoff value was set to 300 amino acids. The protein sequences were then used to search for important domains against a library of Pfam HMM by PfamScan (Madeira, F., et al., The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res, 2019, 47(W1): p. W636-W641). After this filtering step, 131 transposons having a Tn domain remained in the pipeline. 
     Next, the copy number of each transposon was determined using RepeatMasker (Smit, A F A, Hubley, R &amp; Green, P. RepeatMasker Open-4.0.2013-2015). The average sequence divergence value was calculated between the consensus transposon and its family members in each species, and this divergence value can range from 0 to 24.4% in different species. As shown in Table 1, 62 identified TEs have an average divergence value no greater than 5%, and 69 identified TEs have an average divergence value greater than 5%. These 131 active transposons were found to distribute among five superfamilies ( FIG.  2   ) 
     Among the 62 identified transposons in Table 1, a total of 11 transposons were found to meet the following stricter criteria, rendering them highly suitable for use in genome engineering: 1) the length of the transposon is less than 3000 bp; 2) the number of miniature inverted-repeat transposable elements (MITEs) within the transposon is greater than 10; and 3) the average divergence value of the transposon is less than 1% (TE IDs: 4, 5, 7, 12, 18, 20, 22, 25, 26, 28 and 30 as shown in Table 1). 
     Taken together, these data show 131 candidate active transposons identified via a pan-genome bioinformatics analysis of 100 genomes. Thus, this example demonstrates successful development of a robust bioinformatics pipeline for identifying candidate active transposable elements. 
     Example 2: Validation of Candidate Active Transposable Elements 
     This example describes the experimental validation of the candidate active transposable elements identified in Example 1. 
     Materials and Methods 
     DNA Synthesis and Plasmid Construction 
     The mammalian codon-optimized transposase ORFs flanked by EcoRI and NotI were synthesized, and cloned into CMV-hyPBase vector (K. Yusa, et al., A hyperactive piggyBac transposase for mammalian applications. Proc Natl Acad Sci USA, 2011, 108(4): p. 1531-6). These vectors were the helper plasmids, for helping transposase expression under a human CMV promoter. Transposon donor plasmids comprise left and right transposon fragments that flank an antibiotic resistance gene. As used herein, a left transposon fragment (LTF) refers to a fragment from the 5′TSD to the start codon of the transposase ORF sequence of a TE sequence. As used herein, a right transposon fragment (RTF) refers to a fragment from the stop codon of the transposase ORF sequence to the 3′TSD of a TE sequence. Generally, the TR sequences are located within the left and right transposon fragments. For instance, the 5′TR sequence is located within the LTF sequence, and the 3′TR sequence is located within the RTF sequence. LTF and RTF sequences used in this experiment were synthesized by Qinglan Biotechnology Inc., and cloned into pMV vectors. 5′ and 3′ multiple cloning sites (MCSs) were also synthesized within the transposon fragments for cargo gene cloning. TSD sequences were located on the outermost sides. The donor plasmids for transposition screening in mammalian cells, carrying a P2A-linked puromycin resistance gene and an enhanced GFP gene expressed by PGK promoter.  FIG.  3    shows an exemplary set of helper and donor constructs for use in validating active transposable elements. 
     Transposition in Mammalian Cells 
     Four mammalian cell lines, HEK293T (also known as 293T), HeLa, Hct116 and K562, were used to screen for active transposons. 293T and HeLa cell lines were maintained in DMEM medium and supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Hct116 and K562 cell lines were maintained in RPMI 1640 medium and supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. In addition to these cell lines, CD3 +  T cell were isolated using the EasySep Human T Cell Enrichment Kit, following collection of mononuclear cells by histopaque-1077 (Sigma-Aldrich) gradient separation, and CD3′ T cell were cultured in and cultured in X-Vivo 15 mediums (Lonza), supplemented with 5% (v/v) heat-inactivated fetal bovine serum, 2 mM L-glutamine and 1 mM sodium pyruvate. 
     For the transposition assay, 1.2×10 5  HEK293T cells, 0.7×10 5  HeLa cells or 1.0×10 5  Hct116 cells were seeded into individual wells of 24-wells plate 18 h before transfection. Either 200 ng of helper plasmid and 100 ng of donor plasmid, or 100 ng of donor plasmid alone were delivered into each cell line using Lipo3000. Two days after transfection, the number of cells was counted and the transfection efficiency was measured by FACS. Then 1/100 th  transfected HEK293T, 1/10 th  transfected HeLa cells or 1/10 th  transfected Hct116 cells were transferred into 100-mm plates for puromycin (0.5 μg/ml) selection for 10 days (HEK-293T cell line) or 14 days (HeLa cell line and Hct116 cell line). 
     Following puromycin selection, the cells were washed once with 5 ml cold PBS, then fixed by 4% PFA for 15 min, followed by staining with 0.2% methylene blue (in PBS) for 1 h (Wu et al., piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells. PNAS, 2006, 103: p. 15008-15013). Finally, the residual non-specific staining was washed off with PBS. Individual stained colonies were counted by Image J software. According to previously counted total number of transfected cells and transfection efficiency, the transposition efficiency was calculated. 
     For suspension cells, like K562 cell line and CD3+ T cells, transposition activity was assessed based on the percent of GFP positive cells on the 14 th , days after electroporation, when plasmids were been diluted to an extremely small proportion. 
     Construction of the TEs Insertion Library and Bioinformatics Analysis 
     Genome DNA was isolated from stable transposition K562 cells using a DNeasy Blood and Tissue Kit (Qiagen, Germany), sheared to an average length of 600 bp using a Covaris M220 ultrasonicator (Covaris, USA). DNA sample was end-repaired, linker ligation and amplified by nested PCRs was purified and sequenced on an Illumina HiSeq sequencer. Then, TEs integration sites were compared to random insertion sites among primary sequence upstream and downstream region flanking the vector integration site, distance to nearest gene and TSS, and different chromatin states. 
     Results 
     The validation project comprised three rounds of experiments. 
     For the initial round, the 11 TEs with a divergence value less than 1% and a copy number of MITE greater than 10 (i.e., TE IDs: 4, 5, 7, 12, 18, 20, 22, 25, 26, 28 and 30) were first validated. Without wishing to be bound by any theory, it is postulated that a lower divergence value and a higher MITE copy number indicate a higher likelihood of a transposon having been recently active in a species (see R. Mitra, et al., Functional characterization of piggyBat from the bat  Myotis lucifugus  unveils an active mammalian DNA transposon. Proc Natl Acad Sci USA. 2013, 110(1): p. 234-9). 
     During plasmid construction, transposon TR sequences from two different sources were found available, one from the consensus sequence provided by the database, and the other from alignment of autonomous and MITE sequences. To test whether the different TR sequence sources would affect assay results, two sets of donor plasmids were designed using TR sequences from the different sources for the transcription assays, respectively. 
     Results showed that for the 11 candidate TEs tested in the initial validation, three TEs were found to be active ones. Tc1-3_Xt (TE ID 25) was found to be active in both HEK293T and HeLa cells, with a transfer efficiency about 9.6% in HEK293T cells and 3.6% in HeLa cells, equivalent to about half of that of piggyBac (18.4% in HEK293T cells and 8.3% in HeLa cells). Two TEs were found to be active in only one of the two cell lines tested: hAT-3_XT (TE ID 22) was about 1% active in HeLa cells and hAT-5_DR (TE ID 28) was about 2% active in HEK-293T cells. Because no significant differences in transposition efficiency were observed between the two donor plasmid sets having differently sourced TR sequences, later experiments only relied on the TR sequences sourced from consensus sequences in the database. 
     For the following round of experiments, 48 candidate TEs selected from Table 1 were evaluated for transposition activity in 293T and HeLa cell lines (among them, 16 TEs were tested in 293T cells only). With a surprisingly high success rate, 22 TEs were found to be active from the 48 candidate TEs tested. Among them, eight TEs exhibited a transposition efficiency about equal or even higher than piggyBac: Tc1-8B_DR (TE ID 14), Tc1-3 FR (TE ID 29), Mariner2_AG (TE ID 35), Tc1-1_Xt (TE ID 36), Tc1-1_AG (TE ID 37), Tc1-1_PM (TE ID 43), Tc1-4_Xt (TE ID 54), and Tc1-15_Xt (TE ID 56). In reference, piggyBac had a transposition efficiency of 17.44% in 293T cells and 10.25% in HeLa cells. 
     For several active TEs, because the transposition efficiency was much higher than expected, the standard dilution of cells was not enough to keep the surviving colonies separate on the assay plate for an accurate counting of the individual colonies. As a result, the number of stained colonies as counted by the image software was underestimated. For those TEs, even though the staining of the assay plate clearly suggested a much higher transposition efficiency, the calculated transposition efficiency based on the underestimated colony counting was only an underestimation of the actual transposition efficiency. 
     In summary, from two rounds of experiments, 59 candidate TEs were evaluated for transposition activity.  FIGS.  4 A- 5 B  show the transposition efficiencies of the evaluated TEs as compared to control TEs. A total of 25 TEs were found to have activity in human cell lines. 
     Among the 25 active TEs, nine TEs belong to the hAT superfamily and 16 TEs belong to the TcMariner superfamily. Eight active TEs had a transposition activity comparable to or even higher than that of piggyBac. These eight highly efficient active TEs all belong to the TcMariner superfamily, suggesting more active transposons distributed in this superfamily. Further, nine of the 25 active TEs are from tropical clawed frog. In addition, no apparent relationships were observed between the transposition activity and the divergence value or the MITE copy number of a TE. 
     In a 3rd round of experiments, the remaining 72 candidate TEs selected from Table 1 were evaluated for transposition activity in 293T and HeLa cell lines. Among the candidate TEs, 69 TEs with a divergence value greater than 5% were tested in 293T cells only. 13 TEs were found to be active from the 72 candidate TEs tested. Results are shown in  FIGS.  6 A- 6 B . 
     Table 2 summarizes validated active TEs from the above experiments, including a total of 38 TEs were validated active in both 293T cell line and HeLa cell line, or only in one of the two cell lines. Phylogenetic trees of the identified TEs belonging to different superfamilies based on the transposase sequences are shown in  FIGS.  7 A- 7 C . Percentage sequence similarities among the various transposases within each superfamily are shown in Tables 3-5. 
     Notably, 5 DNA TEs (Tc1-8B_DR (TE ID 14), Tc1-3_FR (TE ID 29), Mariner2_AG (TE ID 35), Tc1-1_Xt (TE ID 36), Tc1-1_PM (TE ID 43) show superior transposition activity than SB100X, which has been optimized many times. The top 5 active TEs also contain higher transposition activity among HEK293T cells, Hela cells and HCT116 cells ( FIGS.  5 A- 9 B ), and  FIGS.  10 A- 10 B  show transposition activity of TEs in primary T cells. The phenomenon known as overproduction inhibition (OPI) didn&#39;t occur in the 3 TEs (Tc1-8B_DR (TE ID 14), Tc1-3_FR (TE ID 29), Tc1-1_PM (TE ID 43).  FIGS.  11 A- 11 B  show increased transposition activity with optimized ratio of helper plasmid encoding transposase to transposon plasmid.  FIG.  12    shows the cargo capacity, including 2 kb, 5 kb, 10 kb and 20 kb gene length, of top5 active TEs, compared to identified control TEs, piggyBAC, hyperPiggyBac and SB100X. 
     Comparison of the active TEs with the inactive TEs among the 131 TE candidates show that active TEs exhibited lower average diversity, slightly longer predicted TIR sequence and a significant increase in autonomous TEs numbers. These differences are helpful differentiating features that can be used in the bioinformatics pipeline for identifying active TEs, including both preliminary and large-scale screening settings. 
     The retrieved integration sites at transposon integration sites revealed the highly preferred TA target site dinucleotides for the top 5 active TEs ( FIG.  13   ). The target site dinucleotide of TEs belong to Tc/Mariner superfamily and is very conservative.  FIGS.  14 A- 18    show frequencies of integration into genomic features, including distance to genes and transcription start sites (TSS), different chromatin states, by comparing computer-generated random data and control TEs, piggyBAC. The data show that all of the top 5 active TEs have very low preference for integration near gene sequences and no preference in the upstream and downstream sequences of genes and TSS. With respect to gene expression levels and chromatin states, the top 5 active TEs also show nearly random integration patterns, and they tend to not insert in hyperactive expression regions. 
     Taken together, these data exhibited successful establishment of a robust bioinformatics pipeline for identifying candidate active transposable elements, and successful experimental validation of the identified TEs. The transposition efficiencies and integration patterns of the various TEs suggest that these TEs may be useful in genome engineering applications and gene therapies. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Candidate transposable elements (TEs) identified from the bioinformatics pipeline. 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 TE 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 name/ 
                   
                   
                   
                   
                   
                   
                   
                   
                 Auto, 
                   
                 Total 
                   
                   
                   
               
               
                 TE 
                 Repbase 
                 Super 
                   
                 Length 
                 Diver- 
                   
                   
                   
                 5′ TSD, 
                 copy 
                 MITE 
                 copy 
                   
                   
                 NCBI 
               
               
                 ID 
                 ID 
                 family 
                 Species 
                 (bp) 
                 gence 
                 5′TR 
                 3′TR 
                 Transposase 
                 3′ TSD 
                 # 
                 copy # 
                 # 
                 LTF 
                 RTF 
                 LD 
               
               
                   
               
            
           
           
               
            
               
                 Divergence &lt; 5% 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 1 
                 hAT- 
                 hAT 
                   A.   gambiae   
                 4,80 
                  0.00% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 1 
                 14 
                 15 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 2_AG 
                   
                   
                 3 
                   
                 NO: 1 
                 NO: 27 
                 NO: 53 
                 NO: 191 
                   
                   
                   
                 NO: 115 
                 NO: 141 
                   
               
               
                   
               
               
                 2 
                 AgaP1 
                 P 
                   A.   gambiae   
                 5,64 
                  0.00% 
                 AgaP12:1- 
                 AgaP12:562 
                 AgaP12:529 
                 gtegaaa 
                 1 
                 15 
                 16 
                 AgaP12:1- 
                 AgaP12:2 
                 2445005 
               
               
                   
                 2 
                   
                   
                 9 
                   
                 26 
                 4-5649 
                 -2346 
                 c 
                   
                   
                   
                 528 
                 347-5649 
                 _L2 
               
               
                   
               
               
                 3 
                 P3_AG 
                 P 
                   A.   gambiae   
                 4,39 
                  0.00% 
                 P3_AG:1-29 
                 P3_AG:436 
                 P3_AG:732- 
                 gtcataag 
                 4 
                 2 
                 6 
                 P3_AG:1- 
                 P3_AG:22 
                 2445005 
               
               
                   
                   
                   
                   
                 4 
                   
                   
                 6-4394 
                 2237 
                   
                   
                   
                   
                 731 
                 38-4394 
                 _L3 
               
               
                   
               
               
                 4 
                 HAT2_ 
                 hAT 
                   C.   intestin   
                 2,67 
                  0.00% 
                 HAT2_CI:1- 
                 HAT2 Cl:2 
                 HAT2_CI:4 
                 ATAAT 
                 1 
                 53 
                 54 
                 HAT2CI: 
                 HAT2CI: 
                 2445005 
               
               
                   
                 CI 
                   
                 
                   alis 
                 
                 7 
                   
                 12 
                 666-2677 
                 22-2236 
                 AAT 
                   
                   
                   
                 1-421 
                 2237-2677 
                 _L4 
               
               
                   
               
               
                 5 
                 HAT5_ 
                 hAT 
                 
                   C. 
                 
                 2,21 
                  0.00% 
                 HATS CI:1- 
                 ITAT5 CI:2 
                 HATS CI:2 
                 ATAG 
                 1 
                 14 
                 15 
                 HATS CI: 
                 HAT5 CI: 
                 2445005 
               
               
                   
                 CI 
                   
                 
                   mtestmalis 
                 
                 1 
                   
                 17 
                 195-2211 
                 56-2082 
                 GTAT 
                   
                   
                   
                 1-255 
                 2083-2211 
                 _L5 
               
               
                   
               
               
                 6 
                 hAT- 
                 hAT 
                   D.   rerio   
                 3,52 
                  0.00% 
                 hAT- 
                 hAT- 
                 hAT- 
                 ctatgtat 
                 1 
                 485 
                 486 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 6_DR 
                   
                   
                 9 
                   
                 6_DR:1-12 
                 6_DR:3518- 
                 6_DR:225- 
                   
                   
                   
                   
                 6_DR:1- 
                 6_DR:129 
                 _L6 
               
               
                   
                   
                   
                   
                   
                   
                   
                 3529 
                 1295 
                   
                   
                   
                   
                 224 
                 6-3529 
                   
               
               
                   
               
               
                 7 
                 Chaplin 
                 hAT 
                   D.   rerio   
                 2,92 
                  0.00% 
                 Chaplin1_D 
                 Chaplin1_D 
                 Chaplin1_D 
                 GTCTA 
                 1 
                 281 
                 282 
                 Chaplin1_ 
                 Chaplini 
                 2445005 
               
               
                   
                 1_DR 
                   
                   
                 0 
                   
                 R:1-16 
                 R:2905- 
                 R:314-2251 
                 TAG 
                   
                   
                   
                 DRA-313 
                 DR:2252- 
                 _L7 
               
               
                   
                   
                   
                   
                   
                   
                   
                 2920 
                   
                   
                   
                   
                   
                   
                 2920 
                   
               
               
                   
               
               
                 8 
                 Harbin 
                 PIF- 
                 
                   X. 
                 
                 5,44 
                  0.00% 
                 Harbinger- 
                 Harbinger- 
                 Harbinger- 
                 TTAAC 
                 1 
                 374 
                 375 
                 Harbinger- 
                 Harbinger- 
                 2445005 
               
               
                   
                 ger- 
                 Harbin 
                 
                   tropicalis 
                 
                 2 
                   
                 4_XT:1-30 
                 4_XT:5413- 
                 4_XT:974- 
                 TCTAA 
                   
                   
                   
                 4_XT:1- 
                 4_XT:173 
                 _L8 
               
               
                   
                 4_XT 
                 ger 
                   
                   
                   
                   
                 5442 
                 1729 
                   
                   
                   
                   
                 973 
                 0-5442 
                   
               
               
                   
               
               
                 9 
                 HAT1_ 
                 hAT 
                   A.   gambiae   
                 3,70 
                  0.10% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 3 
                 20 
                 23 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 AG 
                   
                   
                 2 
                   
                 NO: 2 
                 NO: 28 
                 NO: 54 
                 NO: 192 
                   
                   
                   
                 NO: 116 
                 NO: 142 
                   
               
               
                   
               
               
                 10 
                 AgaP1 
                 P 
                   A.   gambiae   
                 5,46 
                  0.10% 
                 AgaP15:1- 
                 AgaP1.5:543 
                 AgaP1.5:971 
                 atatgaac 
                 2 
                 96 
                 98 
                 AgaP15:1- 
                 AgaP15:2 
                 2445005 
               
               
                   
                 5 
                   
                   
                 2 
                   
                 31 
                 2-5462 
                 -2425 
                   
                   
                   
                   
                 970 
                 426-5462 
                 _L10 
               
               
                   
               
               
                 11 
                 IS4EU- 
                 PIF- 
                   D.   rerio   
                 5,00 
                  0.10% 
                 IS4EU- 
                 IS4EU- 
                 IS4EU- 
                 TA 
                 1 
                 2 
                 3 
                 IS4EU- 
                 IS4EU- 
                 2445005 
               
               
                   
                 1_DR 
                 Harbin 
                   
                 4 
                   
                 1_DR:1-11 
                 1_DR:4994- 
                 1_DR:1006- 
                   
                   
                   
                   
                 1_DR:1- 
                 1_DR:209 
                 _L11 
               
               
                   
                   
                 ger 
                   
                   
                   
                   
                 5004 
                 2094 
                   
                   
                   
                   
                 1005 
                 5-5004 
                   
               
               
                   
               
               
                 12 
                 POGO 
                 TcMari 
                 
                   D. 
                 
                 2,12 
                  0.10% 
                 POGO:1-21 
                 POGO:2101 
                 POGO:340- 
                 TA 
                 5 
                 43 
                 48 
                 POGO:1- 
                 POGO:14 
                 2445005 
               
               
                   
                   
                 ner 
                 
                   melano 
                 
                 1 
                   
                   
                 -2121 
                 1458 
                   
                   
                   
                   
                 3.39 
                 59-2121 
                 _L12 
               
               
                   
                   
                   
                 
                   gaster 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 14 
                 Tc1- 
                 TcMari 
                   D.   rerio   
                 1,59 
                  0.20% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 22 
                 1 
                 23 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 8B_DR 
                 ner 
                   
                 7 
                   
                 NO: 3 
                 NO: 29 
                 NO: 55 
                 NO: 193 
                   
                   
                   
                 NO: 117 
                 NO: 143 
                   
               
               
                   
               
               
                 15 
                 HOBO 
                 hAT 
                 
                   D. 
                 
                 3,01 
                  0.20% 
                 HOBO:1-12 
                 HOBO:3005 
                 HOBO:316- 
                 gttcacac 
                 1 
                 59 
                 60 
                 HOBO:1- 
                 HOBO:22 
                 2445005 
               
               
                   
                   
                   
                 
                   persimilis 
                 
                 6 
                   
                   
                 -3016 
                 2250 
                   
                   
                   
                   
                 315 
                 51-3016 
                 _L15 
               
               
                   
               
               
                 17 
                 hAT- 
                 hAT 
                 
                   P. 
                 
                 2,39 
                  0.20% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 21 
                 4 
                 25 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 6_PM 
                   
                 
                   marinus 
                 
                 1 
                   
                 NO: 4 
                 NO: 30 
                 NO: 56 
                 NO: 195 
                   
                   
                   
                 NO: 118 
                 NO: 144 
                   
               
               
                   
               
               
                 18 
                 MARIS 
                 TcMari 
                 
                   S. 
                 
                 1,67 
                  0.20% 
                 MARISPI:1 
                 MARISP1:1 
                 MARISP1:3 
                 TA 
                 2 
                 92 
                 94 
                 MARISPI 
                 MARISPI 
                 2445005 
               
               
                   
                 P1 
                 ner 
                 
                   purpurat 
                 
                 6 
                   
                 -38 
                 639-1676 
                 21-1343 
                   
                   
                   
                   
                 :1-320 
                 :1344- 
                 _L18 
               
               
                   
                   
                   
                 
                   us 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 1676 
                   
               
               
                   
               
               
                 19 
                 hAT- 
                 hAT 
                 
                   x. 
                 
                 2,10 
                  0.20% 
                 N/A 
                 N/A 
                 hAT- 
                 ttatacte 
                 1 
                 1 
                 2 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 7_XT 
                   
                 
                   tropicalis 
                 
                 2 
                   
                   
                   
                 7_XT:143- 
                   
                   
                   
                   
                 7_XT:1- 
                 7_XT:192 
                 _L19 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 1921 
                   
                   
                   
                   
                 142 
                 2-2102 
                   
               
               
                   
               
               
                 20 
                 BARI_ 
                 TcMari 
                 
                   D. 
                 
                 1,72 
                  0.30% 
                 BARLDM: 
                 BARI_DM: 
                 BARLDM: 
                 AT 
                 2 
                 28 
                 30 
                 BARI D 
                 BARI D 
                 2445005 
               
               
                   
                 DM 
                 ner 
                 
                   persimihs 
                 
                 7 
                   
                 1-28 
                 1701-1728 
                 379-1398 
                   
                   
                   
                   
                 M:1-378 
                 M:1399- 
                 _L20 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 1728 
                   
               
               
                   
               
               
                 21 
                 Marine 
                 TcMari 
                 
                   P 
                 
                 1,71 
                  0.30% 
                 N/A 
                 N/A 
                 Mariner- 
                 A 
                 1 
                 0 
                 1 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r- 
                 ner 
                 
                   marinus 
                 
                 7 
                   
                   
                   
                 3_PM:3- 
                   
                   
                   
                   
                 3_PM:1-2 
                 3_PM:171 
                 _L21 
               
               
                   
                 3_PM 
                   
                   
                   
                   
                   
                   
                 1715 
                   
                   
                   
                   
                   
                 6-1717 
                   
               
               
                   
               
               
                 22 
                 hAT- 
                 hAT 
                 
                   x. 
                 
                 2,92 
                  0.30% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 5 
                 326 
                 331 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 3_XT 
                   
                 
                   tropicalis 
                 
                 2 
                   
                 NO: 5 
                 NO: 31 
                 NO: 57 
                 NO: 196 
                   
                   
                   
                 NO: 119 
                 NO: 145 
                   
               
               
                   
               
               
                 23 
                 P1_AG 
                 P 
                 
                   gambiae 
                 
                 4,94 
                  0.40% 
                 P1_AG:1-11 
                 P1_AG:493 
                 PI_AG:781- 
                 GGTG 
                 2 
                 0 
                 2 
                 P1_AG:1- 
                 P1_AG:2.3 
                 2445005 
               
               
                   
                   
                   
                   
                 6 
                   
                   
                 6-4946 
                 2301 
                 CTGG 
                   
                   
                   
                 780 
                 02-4946 
                 _L23 
               
               
                   
               
               
                 24 
                 IS4EU- 
                 PIF- 
                   D.   rerio   
                 5,36 
                  0.40% 
                 IS4EU- 
                 IS4EU- 
                 IS4EU- 
                 ATCAT 
                 3 
                 0 
                 3 
                 IS4EU- 
                 IS4EU- 
                 2445005 
               
               
                   
                 2_DR 
                 Harbin 
                   
                 6 
                   
                 2_DR:1-13 
                 2_DR:5354- 
                 2_DR:1363- 
                 TTATA 
                   
                   
                   
                 2_DR:1- 
                 2_DR:217 
                 _L24 
               
               
                   
                   
                 ger 
                   
                   
                   
                   
                 5366 
                 2172 
                   
                   
                   
                   
                 1362 
                 3-5366 
                   
               
               
                   
               
               
                 25 
                 Tc1- 
                 TcMari 
                 
                   X. 
                 
                 1,61 
                  0.40% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 1,268 
                 106 
                 1,374 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 3_Xt 
                 ner 
                 
                   tropicalis 
                 
                 4 
                   
                 NO: 6 
                 NO: 32 
                 NO: 58 
                 NO: 193 
                   
                   
                   
                 NO: 120 
                 NO: 146 
                   
               
               
                   
               
               
                 26 
                 Marine 
                 TcMari 
                 
                   P. 
                 
                 2,46 
                  0.50% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 5 
                 61 
                 66 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r- 
                 ner 
                 
                   marinus 
                 
                 0 
                   
                 1_PM:1-12 
                 1_PM:2450- 
                 1_PM:287- 
                   
                   
                   
                   
                 1_PM:1- 
                 1_PM:209 
                 _L26 
               
               
                   
                 1_PM 
                   
                   
                   
                   
                   
                 2461 
                 2098 
                   
                   
                   
                   
                 286 
                 9-2461 
                   
               
               
                   
               
               
                 27 
                 P2_AG 
                 P 
                 
                   gambiae 
                 
                 4,19 
                  0.60% 
                 P2_AG:1-27 
                 P2_AG:417 
                 P2_AG:128 
                 GTTTC 
                 1 
                 50 
                 51 
                 P2_AG:1- 
                 P2_AG:23 
                 2445005 
               
               
                   
                   
                   
                   
                 6 
                   
                   
                 0-4196 
                 5-2322 
                 TAG 
                   
                   
                   
                 1284 
                 23-4196 
                 _L27 
               
               
                   
               
               
                 28 
                 hAT- 
                 hAT 
                   D.   rerio   
                 2,49 
                 0,60% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 4 
                 21 
                 25 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 5_DR 
                   
                   
                 6 
                   
                 NO: 7 
                 NO: 33 
                 NO: 59 
                 NO: 194 
                   
                   
                   
                 NO: 121 
                 NO: 147 
                   
               
               
                   
               
               
                 29 
                 Tc1- 
                 TcMari 
                 
                   T. 
                 
                 1,59 
                  0.70% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 6 
                 4 
                 10 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 3_FR 
                 ner 
                 
                   rubripes 
                 
                 5 
                   
                 NO: 8 
                 NO: 34 
                 NO: 60 
                 NO: 193 
                   
                   
                   
                 NO: 122 
                 NO: 148 
                   
               
               
                   
               
               
                 30 
                 Marine 
                 TcMari 
                 
                   Ai 
                 
                 2,61 
                  0.70% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 1 
                 229 
                 230 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r-4_XT 
                 ner 
                 
                   tropicalis 
                 
                 3 
                   
                 1X1:1-19 
                 4_XT:2595- 
                 4_XT:1030- 
                   
                   
                   
                   
                 4_XT:1- 
                 4_XT:233 
                 _L30 
               
               
                   
                   
                   
                   
                   
                   
                   
                 2613 
                 2331 
                   
                   
                   
                   
                 1029 
                 2-2613 
                   
               
               
                   
               
               
                 32 
                 Tc1- 
                 TcMari 
                 
                   X. 
                 
                 1,59 
                  1.10% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 377 
                 98 
                 475 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 5_Xt 
                 ner 
                 
                   tropicalis 
                 
                 4 
                   
                 NO: 9 
                 NO: 35 
                 NO: 61 
                 NO: 193 
                   
                   
                   
                 NO: 123 
                 NO: 149 
                   
               
               
                   
               
               
                 33 
                 Tc1- 
                 TcMar- 
                 
                   X. 
                 
                 1606 
                  1.30% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 1,330 
                 323 
                 1,653 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 10_Xt 
                 Tcl 
                 
                   tropicalis 
                 
                   
                   
                 NO: 10 
                 NO: 36 
                 NO: 62 
                 NO: 193 
                   
                   
                   
                 NO: 124 
                 NO: 150 
                   
               
               
                   
               
               
                 34 
                 hAT- 
                 hAT 
                 
                   D. 
                 
                 2,69 
                  1.40% 
                 hAT- 
                 hAT- 
                 hAT- 
                 atgcacat 
                 2 
                 50 
                 52 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 1_DP 
                   
                 
                   persimitis 
                 
                 9 
                   
                 1 DP:1-8 
                 1 DP:2692- 
                 1 DP:417- 
                   
                   
                   
                   
                 1 DP:1- 
                 1 DP:220 
                 _L34 
               
               
                   
                   
                   
                   
                   
                   
                   
                 2699 
                 2207 
                   
                   
                   
                   
                 416 
                 8-2699 
                   
               
               
                   
               
               
                 35 
                 Marine 
                 TcMari 
                 
                   A. 
                 
                 2,00 
                  1.50% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 8 
                 2 
                 10 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 r2_AG 
                 ner 
                 
                   gambiae 
                 
                 0 
                   
                 NO: 11 
                 NO: 37 
                 NO: 63 
                 NO: 193 
                   
                   
                   
                 NO: 125 
                 NO: 151 
                   
               
               
                   
               
               
                 36 
                 Tc1- 
                 TcMari 
                   X.   laevis   
                 1,63 
                  1.50% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 2 
                 272 
                 274 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 1_Xt 
                 ner 
                   
                 4 
                   
                 NO: 12 
                 NO: 38 
                 NO: 64 
                 NO: 193 
                   
                   
                   
                 NO: 126 
                 NO: 152 
                   
               
               
                   
               
               
                 37 
                 Tc1- 
                 TcMari 
                 
                   A. 
                 
                 1,41 
                  1.60% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 30 
                 52 
                 82 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 i_AG 
                 ner 
                 
                   gambiae 
                 
                 3 
                   
                 NO: 13 
                 NO: 39 
                 NO: 65 
                 NO: 193 
                   
                   
                   
                 NO: 127 
                 NO: 153 
                   
               
               
                   
               
               
                 38 
                 Marine 
                 TcMari 
                 
                   X. 
                 
                 1,76 
                  1.60% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 3 
                 0 
                 3 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r-1_XT 
                 ner 
                 
                   tropicalis 
                 
                 9 
                   
                 1_XT:1-3 
                 1_XT:1767- 
                 1_XT:81- 
                   
                   
                   
                   
                 1_XT:1- 
                 1_XT:143 
                 _L38 
               
               
                   
                   
                   
                   
                   
                   
                   
                 1769 
                 1436 
                   
                   
                   
                   
                 80 
                 7-1769 
                   
               
               
                   
               
               
                 39 
                 TclDR 
                 TcMari 
                 
                   X. 
                 
                 1,23 
                  1.70% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 23 
                 103 
                 126 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 3_Xt 
                 ner 
                 
                   tropicalis 
                 
                 1 
                   
                 NO: 14 
                 NO: 40 
                 NO: 66 
                 NO: 198 
                   
                   
                   
                 NO: 128 
                 NO: 154 
                   
               
               
                   
               
               
                 40 
                 hAT- 
                 hAT 
                 
                   P. 
                 
                 2,44 
                  1.80% 
                 AT- 
                 AT- 
                 AT- 
                 CTCTA 
                 13 
                 336 
                 349 
                 AT- 
                 hAT- 
                 2445005 
               
               
                   
                 1B_PM 
                   
                 
                   marinus 
                 
                 6 
                   
                 IB_PM:1- 
                 1B_PM:243 
                 IB_PM:111 
                 GAG 
                   
                   
                   
                 IB_PM:1- 
                 IB_PM:22 
                 _L40 
               
               
                   
                   
                   
                   
                   
                   
                 13 
                 5-2447 
                 6-2210 
                   
                   
                   
                   
                 1115 
                 11-2447 
                   
               
               
                   
               
               
                 41 
                 hAT- 
                 hAT 
                 
                   P. 
                 
                 2,98 
                  1.90% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 10 
                 149 
                 159 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 3_PM 
                   
                 
                   marinus 
                 
                 4 
                   
                 NO: 15 
                 NO: 41 
                 NO: 67 
                 NO: 199 
                   
                   
                   
                 NO: 129 
                 NO: 155 
                   
               
               
                   
               
               
                 42 
                 hAT- 
                 hAT 
                 
                   P. 
                 
                 2,55 
                  1.90% 
                 hAT- 
                 hAT- 
                 hAT- 
                 CTCTA 
                 24 
                 178 
                 202 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 613_PM 
                   
                 
                   marinus 
                 
                 8 
                   
                 6B_PM:1- 
                 6B_PM:254 
                 6B_PM:112 
                 CAC 
                   
                   
                   
                 6B_PM:1- 
                 6B_PM:23 
                 _L42 
               
               
                   
                   
                   
                   
                   
                   
                 16 
                 3-2558 
                 0-2337 
                   
                   
                   
                   
                 1119 
                 38-2558 
                   
               
               
                   
               
               
                 43 
                 Tc1- 
                 TcMari 
                 
                   P. 
                 
                 1,60 
                  2.00% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 6,205 
                 1,258 
                 7,463 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 1_PM 
                 ner 
                 
                   marinus 
                 
                 7 
                   
                 NO: 16 
                 NO: 42 
                 NO: 68 
                 NO: 193 
                   
                   
                   
                 NO: 130 
                 NO: 156 
                   
               
               
                   
               
               
                 44 
                 TC1_X 
                 TcMari 
                   X.   laevis   
                 1,35 
                  2.00% 
                 TCI XL:1- 
                 TCI XL:12 
                 TCI_XL:25 
                 TA 
                 220 
                 52 
                 272 
                 TCI XL:1 
                 TCI XL:1 
                 2445005 
               
               
                   
                 L 
                 ner 
                   
                 6 
                   
                 132 
                 26-1356 
                 6-1185 
                   
                   
                   
                   
                 -255 
                 186-1356 
                 _L44 
               
               
                   
               
               
                 45 
                 Marine 
                 TcMari 
                 
                   mississip 
                 
                 2,12 
                  2.10% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 4 
                 245 
                 249 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 r- 
                 ner 
                 
                   piensis 
                 
                 1 
                   
                 NO: 17 
                 NO: 43 
                 NO: 69 
                 NO: 193 
                   
                   
                   
                 NO: 131 
                 NO: 157 
                   
               
               
                   
                 4_AMi 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 46 
                 Myotis 
                 hAT 
                 
                   M. 
                 
                 2,92 
                  2.10% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 74 
                 18,867 
                 18,94 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 hATl 
                   
                 
                   lucifugus 
                 
                 1 
                   
                 NO: 18 
                 NO: 44 
                 NO: 70 
                 NO: 197 
                   
                   
                 1 
                 NO: 132 
                 NO: 158 
                   
               
               
                   
               
               
                 47 
                 hAT- 
                 hAT 
                 
                   P. 
                 
                 2,49 
                  2.20% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 26 
                 343 
                 369 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 7_PM 
                   
                 
                   marinus 
                 
                 9 
                   
                 NO: 19 
                 NO: 45 
                 NO: 71 
                 NO: 200 
                   
                   
                   
                 NO: 133 
                 NO: 159 
                   
               
               
                   
               
               
                 48 
                 TC1_F 
                 TcMari 
                 
                   T 
                 
                 1,63 
                  2.30% 
                 TCI_FR4:1- 
                 TC1_FR4:1 
                 TC1_FR4:3 
                 TA 
                 14 
                 8 
                 22 
                 TC1_FR4: 
                 TC1_FR4: 
                 2445005 
               
               
                   
                 R4 
                 ner 
                 
                   rubripes 
                 
                 1 
                   
                 27 
                 605-1631 
                 57-1403 
                   
                   
                   
                   
                 1-356 
                 1404-1631 
                 _L48 
               
               
                   
               
               
                 49 
                 hAT- 
                 hAT 
                 
                   P. 
                 
                 3,78 
                  2.30% 
                 hAT- 
                 hAT- 
                 hAT- 
                 CTCTA 
                 8 
                 38 
                 46 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 8_PM 
                   
                 
                   marinus 
                 
                 1 
                   
                 8_PM:1-16 
                 8_PM:3766- 
                 8_PM:2365- 
                 CAG 
                   
                   
                   
                 8_PM:1- 
                 8_PM:361 
                 _L49 
               
               
                   
                   
                   
                   
                   
                   
                   
                 3781 
                 3615 
                   
                   
                   
                   
                 2364 
                 6-3781 
                   
               
               
                   
               
               
                 50 
                 piggyB 
                 piggyB 
                 
                   M. 
                 
                 2,52 
                  2.50% 
                 piggyBac1 
                 piggyBac1 
                 piggyBac1 
                 TTAA 
                 1 
                 1,255 
                 1,256 
                 piggyBac 1 
                 piggyBac1 
                 2445005 
               
               
                   
                 acl_M 
                 ac 
                 
                   murinus 
                 
                 7 
                   
                 Mm:1-13 
                 Mm:2515- 
                 Mm:1037- 
                   
                   
                   
                   
                 Mtn:]- 
                 Mm:234 
                 _L50 
               
               
                   
                 m 
                   
                   
                   
                   
                   
                 2527 
                 2347 
                   
                   
                   
                   
                 1036 
                 8-2527 
                   
               
               
                   
               
               
                 51 
                 Tc1- 
                 TcMari 
                 
                   X. 
                 
                 1633 
                  2.50% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 121 
                 112 
                 233 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 11_Xt 
                 ner 
                 
                   tropicaiis 
                 
                   
                   
                 NO: 20 
                 NO: 46 
                 NO: 72 
                 NO: 193 
                   
                   
                   
                 NO: 134 
                 NO: 160 
                   
               
               
                   
               
               
                 52 
                 Tc1- 
                 TcMari 
                 
                   X 
                 
                 1635 
                  2.80% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 66 
                 61 
                 127 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 16_Xt 
                 ner 
                 
                   tropicaiis 
                 
                   
                   
                 NO: 21 
                 NO: 47 
                 NO: 73 
                 NO: 193 
                   
                   
                   
                 NO: 135 
                 NO: 161 
                   
               
               
                   
               
               
                 53 
                 TC1- 
                 TcMari 
                 
                   D. 
                 
                 1,64 
                  2.90% 
                 TC1- 
                 TCI- 
                 TCI- 
                 TA 
                 3 
                 9 
                 12 
                 TCI- 
                 TC1- 
                 2445005 
               
               
                   
                 2_DM 
                 ner 
                 
                   meianoga 
                 
                 4 
                   
                 2_DM:1-21 
                 2_DM:1624 
                 2_DM:356- 
                   
                   
                   
                   
                 2_DM:1- 
                 2_DM:137 
                 _L53 
               
               
                   
                   
                   
                 
                   ster 
                 
                   
                   
                   
                 -1644 
                 1378 
                   
                   
                   
                   
                 355 
                 9-1644 
                   
               
               
                   
               
               
                 54 
                 Tc1- 
                 TcMari 
                 
                   X 
                 
                 1596 
                  3.30% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 61 
                 71 
                 132 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 4_Xt 
                 ner 
                 
                   tropicaiis 
                 
                   
                   
                 NO: 22 
                 NO: 48 
                 NO: 74 
                 NO: 193 
                   
                   
                   
                 NO: 136 
                 NO: 162 
                   
               
               
                   
               
               
                 55 
                 TCID 
                 TcMari 
                 
                   D. 
                 
                 1,66 
                  3.50% 
                 TC1_DM:1- 
                 TCI_DM:16 
                 TC1_DM:35 
                 TA 
                 4 
                 21 
                 25 
                 TC1_DM: 
                 TCI_DM: 
                 2445005 
               
               
                   
                 M 
                 ner 
                 
                   meianoga 
                 
                 6 
                   
                 26 
                 41-1666 
                 9-1354 
                   
                   
                   
                   
                 1-358 
                 1355-1666 
                 _L55 
               
               
                   
                   
                   
                 
                   ster 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 56 
                 Tc1- 
                 TcMari 
                 
                   X. 
                 
                 1581 
                  3.50% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 60 
                 76 
                 136 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 15_Xt 
                 ner 
                 
                   tropicaiis 
                 
                   
                   
                 NO: 23 
                 NO: 49 
                 NO: 75 
                 NO: 193 
                   
                   
                   
                 NO: 137 
                 NO: 163 
                   
               
               
                   
               
               
                 59 
                 Tc1_F 
                 TcMari 
                 
                   T 
                 
                 1602 
                  3.80% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 15 
                 36 
                 51 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 R2 
                 ner 
                 
                   rubripes 
                 
                   
                   
                 NO: 24 
                 NO: 50 
                 NO: 76 
                 NO: 193 
                   
                   
                   
                 NO: 138 
                 NO: 164 
                   
               
               
                   
               
               
                 60 
                 Marine 
                 TcMari 
                 
                   A 
                 
                 1,96 
                  4.40% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 3 
                 202 
                 205 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r- 
                 ner 
                 
                   mississip 
                 
                 1 
                   
                 6B_AMi:1- 
                 6B_AMi:19 
                 6B_AMi:39 
                   
                   
                   
                   
                 6B_AMi:1 
                 6B_AMi:1 
                 _L60 
               
               
                   
                 6B_AMi 
                   
                 
                   piensis 
                 
                   
                   
                 25 
                 37-1961 
                 0-1649 
                   
                   
                   
                   
                 -389 
                 650-1961 
                   
               
               
                 61 
                 Tc1- 
                 TcMari 
                 
                   X. 
                 
                 1,65 
                  4.40% 
                 Tc1-9_Xt: 
                 Tc1- 
                 Tc1- 
                 TA 
                 14 
                 25 
                 39 
                 Tc1- 
                 Tc1- 
                 2445005 
               
               
                   
                 9_Xt 
                 ner 
                 
                   tropicalis 
                 
                 4 
                   
                 1-225 
                 9_Xt:1430- 
                 9_Xt:362- 
                   
                   
                   
                   
                 9_Xt:1- 
                 9_Xt:1400 
                 _L61 
               
               
                   
                   
                   
                   
                   
                   
                   
                 1654 
                 1399 
                   
                   
                   
                   
                 361 
                 -1654 
                   
               
               
                   
               
               
                 63 
                 hAT- 
                 hAT 
                 
                   X. 
                 
                 5716 
                  4.60% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 124 
                 5,189 
                 5,313 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 9_XT 
                   
                 
                   tropicalis 
                 
                   
                   
                 NO: 25 
                 NO: 51 
                 NO: 77 
                 NO: 201 
                   
                   
                   
                 NO: 139 
                 NO: 165 
                   
               
               
                   
               
               
                 64 
                 Marine 
                 TcMari 
                 
                   X 
                 
                 1840 
                  4.60% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 2 
                 4 
                 6 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 r-5_XT 
                 ner 
                 
                   tropicalis 
                 
                   
                   
                 NO: 26 
                 NO: 52 
                 NO: 78 
                 NO: 193 
                   
                   
                   
                 NO: 140 
                 NO: 166 
                   
               
               
                   
               
               
                 65 
                 S2_DM 
                 TcMari 
                 
                   D. 
                 
                 1,73 
                  4.70% 
                 S2_DM:1- 
                 S2_DM:169 
                 S2_DM:403 
                 TA 
                 2 
                 2 
                 4 
                 S2_DM:1- 
                 S2_DM:1 
                 2445005 
               
               
                   
                   
                 ner 
                 
                   meianoga 
                 
                 5 
                   
                 46 
                 0-1735 
                 -1386 
                   
                   
                   
                   
                 402 
                 387-1735 
                 _L65 
               
               
                   
                   
                   
                 
                   ster 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 67 
                 PROT 
                 p 
                 
                   D. 
                 
                 1,10 
                  4.90% 
                 PROTOP:1- 
                 PROTOP:44 
                 PROTOP:11 
                 GTCCT 
                 8 
                 461 
                 469 
                 PROTOP: 
                 PROTOP: 
                 2445005 
               
               
                   
                 OP 
                   
                 
                   meianoga 
                 
                 5 
                   
                 31 
                 50-4480 
                 94-3788 
                 GG 
                   
                   
                   
                 1-1193 
                 3789-4480 
                 _L67 
               
               
                   
                   
                   
                 
                   ster 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 68 
                 Tc1- 
                 TcMari 
                 
                   a 
                 
                 1,65 
                  4.90% 
                 Tc1-8_Xt:1- 
                 Tc1- 
                 Tc1- 
                 TA 
                 17 
                 81 
                 98 
                 Tc1- 
                 Tc1- 
                 2445005 
               
               
                   
                 8_Xt 
                 ner 
                 
                   tropicalis 
                 
                 0 
                   
                 31 
                 8_Xt:1620- 
                 8_Xt:400- 
                   
                   
                   
                   
                 8_Xt:1- 
                 8_Xt:1423 
                 _L68 
               
               
                   
                   
                   
                   
                   
                   
                   
                 1650 
                 1422 
                   
                   
                   
                   
                 399 
                 -1650 
                   
               
               
                   
               
               
                 69 
                 Tc1- 
                 TcMari 
                 
                   X. 
                 
                 1,62 
                  5.00% 
                 Tc1- 
                 Tc1- 
                 Tc1- 
                 TA 
                 5 
                 22 
                 27 
                 Tc1- 
                 Tc1- 
                 2445005 
               
               
                   
                 12_Xt 
                 ner 
                 
                   tropicalis 
                 
                 6 
                   
                 12_Xt:1-74 
                 12_Xt:1554- 
                 12_Xt:390- 
                   
                   
                   
                   
                 12_Xt:1- 
                 12_Xt:141 
                 E69 
               
               
                   
                   
                   
                   
                   
                   
                   
                 1626 
                 1409 
                   
                   
                   
                   
                 389 
                 0-1626 
                   
               
               
                   
               
            
           
           
               
            
               
                 Divergence &gt; 5% 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 70 
                 Mari 
                 TcMar- 
                 
                   A. 
                 
                 2479 
                  5.10% 
                 SEQ ID 
                 SEQ ID 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 ner- 
                 Tc2 
                 
                   mississip 
                 
                   
                   
                 NO: 79 
                 NO: 91 
                 NO: 103 
                 NO: 193 
                   
                   
                   
                 NO: 167 
                 NO: 179 
                   
               
               
                   
                 6_AMi 
                   
                 
                   piensis 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 71 
                 hAT- 
                 hAT 
                 
                   mississip 
                 
                 3,32 
                  5.50% 
                 hAT- 
                 hAT- 
                 hAT- 
                 CTTGT 
                 7 
                 118 
                 125 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 3_Gm 
                   
                 
                   piensis 
                 
                 5 
                   
                 3_Gav:1-10 
                 3_Gav:3316 
                 3_Gav:1577 
                 ATC 
                   
                   
                   
                 3_Gav:1- 
                 3_Gav:30 
                 _L71 
               
               
                   
                   
                   
                   
                   
                   
                   
                 -3325 
                 -3028 
                   
                   
                   
                   
                 1576 
                 29-3325 
                   
               
               
                   
               
               
                 72 
                 piggyB 
                 piggyB 
                 
                   M. 
                 
                 2,21 
                  5.70% 
                 N/A 
                 N/A 
                 piggyBac2 
                 CTATA 
                 0 
                 0 
                 0 
                 piggyBac2 
                 piggyBac2 
                 2445005 
               
               
                   
                 ac2_M 
                 ac 
                 
                   murinus 
                 
                 1 
                   
                   
                   
                 Mm:277- 
                 TAG 
                   
                   
                   
                 Mm:1- 
                 Mm:208 
                 _L72 
               
               
                   
                 m 
                   
                   
                   
                   
                   
                   
                 2085 
                   
                   
                   
                   
                 276 
                 6-2211 
                   
               
               
                   
               
               
                 73 
                 Marine 
                 TcMari 
                 
                   X. 
                 
                 2,43 
                  5.70% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TCTAT 
                 1 
                 306 
                 307 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r-2_XT 
                 ner 
                 
                   tropicalis 
                 
                 8 
                   
                 2_XT:1-27 
                 2_XT:2412- 
                 2_XT:764- 
                 GAC 
                   
                   
                   
                 2_XT:1- 
                 2_XT:212 
                 _L73 
               
               
                   
                   
                   
                   
                   
                   
                   
                 2438 
                 2128 
                   
                   
                   
                   
                 763 
                 9-2438 
                   
               
               
                   
               
               
                 74 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 3,50 
                  5.80% 
                 hAT- 
                 hAT- 
                 hAT- 
                 TA 
                 3 
                 9 
                 12 
                 4_Crp:1- 
                 4_Crp:324 
                 2445005 
               
               
                   
                 4_Crp 
                   
                 
                   mississip 
                 
                 8 
                   
                 4_Crp:1-14 
                 4_Crp:3495- 
                 4_Crp:603- 
                   
                   
                   
                   
                 602 
                 0-3508 
                 _L74 
               
               
                   
                   
                   
                 
                   piensis 
                 
                   
                   
                   
                 3508 
                 3239 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 75 
                 Opos 
                 hAT 
                 
                   M. 
                 
                 2,99 
                  5.80% 
                 OposCharlie 
                 OposCharlie 
                 OposCharlie 
                 GTCAT 
                 17 
                 1172 
                 1189 
                 OposCharl 
                 OposCharl 
                 2445005 
               
               
                   
                 Charlie 
                   
                 
                   domestic 
                 
                 6 
                   
                 2:1-16 
                 2:2981-2996 
                 2:744-2681 
                 AAG 
                   
                   
                   
                 ie2:1-743 
                 ie2:2682- 
                 _L75 
               
               
                   
                 2 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 2996 
                   
               
               
                   
               
               
                 76 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 3,50 
                  6.00% 
                 hAT- 
                 hAT- 
                 hAT- 
                 ITTCC 
                 16 
                 43 
                 59 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 1_AMi 
                   
                 
                   mississip 
                 
                   
                   
                 1_AMi:1-9 
                 1_AMi:3499 
                 1_AMi:1102 
                 TTG 
                   
                   
                   
                 1_AMi:1- 
                 1_AMi:29 
                 _L76 
               
               
                   
                   
                   
                 
                   piensis 
                 
                   
                   
                   
                 -3507 
                 -2943 
                   
                   
                   
                   
                 1101 
                 44-3507 
                   
               
               
                   
               
               
                 77 
                 Mariner 
                 TcMari 
                 
                   A 
                 
                 2,26 
                  6.00% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 1 
                 99 
                 100 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 2_AMi 
                 ner 
                 
                   mississip 
                 
                 0 
                   
                 2_AMi:1-13 
                 2_AMi:2248 
                 2_AMi:587- 
                   
                   
                   
                   
                 2_AMi:1- 
                 2_AMi:20 
                 _L77 
               
               
                   
                   
                   
                 
                   piensis 
                 
                   
                   
                   
                 -2260 
                 2065 
                   
                   
                   
                   
                 586 
                 66-2260 
                   
               
               
                   
               
               
                 78 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 3.53 
                  6.40% 
                 hAT- 
                 hAT- 
                 hAT- 
                 TA 
                 24 
                 186 
                 210 
                 hAT- 
                 AT- 
                 2445005 
               
               
                   
                 13_ 
                   
                 
                   mississip 
                 
                 1 
                   
                 13_AMi:1- 
                 13_AMi:350 
                 13_AMi:125 
                   
                   
                   
                   
                 13_AMi:1 
                 13_AMi:3 
                 _L78 
               
               
                   
                 AMii 
                   
                 
                   piensis 
                 
                   
                   
                 29 
                 4-3531 
                 5-3087 
                   
                   
                   
                   
                 -1254 
                 088-35.31 
                   
               
               
                   
               
               
                 80 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 4,34 
                  6.60% 
                 hAT- 
                 hAT- 
                 hAT- 
                 TTAA 
                 2 
                 14 
                 16 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 12_AM 
                   
                 
                   mississip 
                 
                 8 
                   
                 12_AMi:1- 
                 12_AMi:433 
                 12_AMi:139 
                   
                   
                   
                   
                 12_AMi:1 
                 12_AMi:3 
                 _L80 
               
               
                   
                 i 
                   
                 
                   piensis 
                 
                   
                   
                   
                 2-4348 
                 2-3989 
                   
                   
                   
                   
                 -1391 
                 990-4348 
                   
               
               
                   
               
               
                 81 
                 Mari 
                 TcMari 
                 
                   A. 
                 
                 2,33 
                  6.70% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 6 
                 347 
                 353 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 ner- 
                 ner 
                 
                   mississip 
                 
                 5 
                   
                 7_Croc:1-17 
                 7_Croc:231 
                 7_Croc:195- 
                   
                   
                   
                   
                 7_Croc:1- 
                 7_Croc:19 
                 _L81 
               
               
                   
                 7_Croc 
                   
                 
                   piensis 
                 
                   
                   
                   
                 9-2335 
                 1982 
                   
                   
                   
                   
                 194 
                 83-2335 
                   
               
               
                   
               
               
                 82 
                 piggyB 
                 PiggyB 
                 
                   tropicalis 
                 
                 8451 
                  6.70% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 0 
                 19 
                 19 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 ac- 
                 ac 
                   
                   
                   
                 NO: 80 
                 NO: 92 
                 NO: 104 
                 NO: 202 
                   
                   
                   
                 NO: 168 
                 NO: 180 
                   
               
               
                   
                 2_XT 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 83 
                 Mari 
                 TcMari 
                 
                   A. 
                 
                 2,52 
                  6.80% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 0 
                 190 
                 190 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 ner- 
                 ner 
                 
                   mississip 
                 
                 2 
                   
                 3_AMi:1-24 
                 3_AMi:2499 
                 3_AMi:345- 
                   
                   
                   
                   
                 3_AMi:1- 
                 3_AMi:21 
                 _L83 
               
               
                   
                 3_AMi 
                   
                 
                   piensis 
                 
                   
                   
                   
                 -2522 
                 2138 
                   
                   
                   
                   
                 344 
                 39-2522 
                   
               
               
                   
               
               
                 84 
                 piggvB 
                 piggyB 
                 
                   C. 
                 
                 2,21 
                  6.80% 
                 piggyBac1_ 
                 piggy Bad 
                 piggyBac1_ 
                 GCTGC 
                 2 
                 2 
                 A 
                 piggyBac1 
                 piggyBac1 
                 2445005 
               
               
                   
                 ael_CI 
                 ac 
                 
                   intestinali 
                 
                 6 
                   
                 CI:1-13 
                 CI:2204- 
                 CE263-1984 
                 AGG 
                   
                   
                   
                 CI:1-262 
                 CI:1985- 
                 _L84 
               
               
                   
                   
                   
                   
                   
                   
                   
                 2216 
                   
                   
                   
                   
                   
                   
                 2216 
                   
               
               
                   
               
               
                 86 
                 piggyB 
                 PiggyB 
                 
                   A. 
                 
                 3224 
                  7.00% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 20 
                 112 
                 132 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 ac- 
                 ac 
                 
                   mississip 
                 
                   
                   
                 NO: 81 
                 NO: 93 
                 NO: 105 
                 NO: 202 
                   
                   
                   
                 NO: 169 
                 NO: 181 
                   
               
               
                   
                 1_AMi 
                   
                 
                   piensis 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 87 
                 Marine 
                 TcMari 
                 
                   A. 
                 
                 2,29 
                  7.00% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 GTTGT 
                 2 
                 2105 
                 2107 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r- 
                 ner 
                 
                   mississip 
                 
                 4 
                   
                 5_AMi:1-21 
                 5_AMi:2274 
                 5_AMi:535- 
                 AAC 
                   
                   
                   
                 5_AMi:1- 
                 5_AMi:20 
                 _L87 
               
               
                   
                 5_AMi 
                   
                 
                   piensis 
                 
                   
                   
                   
                 -2294 
                 2076 
                   
                   
                   
                   
                 534 
                 77-2294 
                   
               
               
                   
               
               
                 89 
                 Marine 
                 TcMari 
                 
                   A. 
                 
                 2,24 
                  7.30% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 5 
                 715 
                 720 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r- 
                 ner 
                 
                   mississip 
                 
                 9 
                   
                 6_Crp:1-24 
                 6_Crp:2226- 
                 6_Crp:240- 
                   
                   
                   
                   
                 6_Crp:1- 
                 6_Crp:205 
                 _L89 
               
               
                   
                 6_Crp 
                   
                 
                   piensis 
                 
                   
                   
                   
                 2249 
                 2051 
                   
                   
                   
                   
                 239 
                 2-2249 
                   
               
               
                   
               
               
                 90 
                 Marine 
                 TeMar- 
                 
                   A. 
                 
                 2292 
                  7.50% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 9 
                 25 
                 34 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 r- 
                 Tigger 
                 
                   mississip 
                 
                   
                   
                 NO: 82 
                 NO: 94 
                 NO: 106 
                 NO: 193 
                   
                   
                   
                 NO: 170 
                 NO: 182 
                   
               
               
                   
                 3_Crp 
                   
                 
                   piensis 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 91 
                 hAT- 
                 hAT 
                 
                   mississip 
                 
                 3,56 
                  7.60% 
                 hAT- 
                 hAT- 
                 hAT- 
                 ACTGT 
                 2 
                 41 
                 43 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 3_AMi 
                   
                 
                   piensis 
                 
                 5 
                   
                 3_AMi:1-14 
                 3_AMi:3552 
                 3_AMi:1179 
                 TCTAT 
                   
                   
                   
                 3_AMi:1- 
                 3_AMi:30 
                 _L91 
               
               
                   
                   
                   
                   
                   
                   
                   
                 -3565 
                 -3029 
                   
                   
                   
                   
                   
                 30-3565 
                   
               
               
                   
               
               
                 92 
                 TC1_F 
                 TcMari 
                 
                   T. 
                 
                 1,22 
                  7.80% 
                 TCI FRL:1- 
                 TCI FR1:1 
                 TCI FR1:1 
                 CCTTG 
                 6 
                 23 
                 29 
                 TC1_FR1: 
                 TCI FRI: 
                 2445005 
               
               
                   
                 RI 
                 ner 
                 
                   rubripes 
                 
                 0 
                   
                 24 
                 197-1220 
                 48-1188 
                 AAG 
                   
                   
                   
                 1-147 
                 1189-1220 
                 E92 
               
               
                   
               
               
                 93 
                 hAT- 
                 hAT 
                 
                   mississip 
                 
                 3,51 
                  7.90% 
                 hAT- 
                 hAT- 
                 hAT- 
                 CTATA 
                 2 
                 327 
                 329 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 5_Croc 
                   
                 
                   piensis 
                 
                 9 
                   
                 5_Croc:1-10 
                 5_Croc:351 
                 5_Croc:140 
                 TAG 
                   
                   
                   
                 5_Croc:1- 
                 5_Croc:29 
                 _L93 
               
               
                   
                   
                   
                   
                   
                   
                   
                 0-3519 
                 3-2.905 
                   
                   
                   
                   
                 1402 
                 06-3519 
                   
               
               
                   
               
               
                 94 
                 hA_T- 
                 hAT 
                 
                   mississip 
                 
                 3,33 
                  7.90% 
                 hAT- 
                 hAT- 
                 hAT- 
                 TA 
                 0 
                 57 
                 57 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 8_AMi 
                   
                 
                   piensis 
                 
                 2 
                   
                 8_AMi:1-16 
                 8_AMi:33T7 
                 8_AMi:1159 
                   
                   
                   
                   
                 8_AMi:1- 
                 8_AMi:31 
                 _L94 
               
               
                   
                   
                   
                   
                   
                   
                   
                 -3332 
                 -3183 
                   
                   
                   
                   
                 1158 
                 84-3332 
                   
               
               
                   
               
               
                 95 
                 PARIS 
                 TcMari 
                   D.   virilis   
                 1,73 
                  7.90% 
                 PARIS:1- 
                 PARIS:1489 
                 PARIS:394- 
                 CTGTA 
                 2 
                 6 
                 8 
                 PARIS:1- 
                 PARIS:14 
                 2445005 
               
               
                   
                   
                 ner 
                   
                 0 
                   
                 242 
                 -1730 
                 1440 
                 TAG 
                   
                   
                   
                 393 
                 41-1730 
                 _L95 
               
               
                   
               
               
                 96 
                 Mari 
                 TcMari 
                 
                   P. 
                 
                 2,39 
                  7.90% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TA 
                 3 
                 226 
                 229 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 ner- 
                 ner 
                 
                   marinus 
                 
                 0 
                   
                 2_PM:1-23 
                 2_PM:2368- 
                 2_PM:288- 
                   
                   
                   
                   
                 2_PM:1- 
                 2_PM:168 
                 _L96 
               
               
                   
                 2_PM 
                   
                   
                   
                   
                   
                 2390 
                 1682 
                   
                   
                   
                   
                 287 
                 3-2390 
                   
               
               
                   
               
               
                 97 
                 piggyB 
                 piggyB 
                 
                   X 
                 
                 6,16 
                  8.30% 
                 piggyBac- 
                 piggyBac- 
                 piggyBac- 
                 CTGTA 
                 1 
                 301 
                 302 
                 piggyBac- 
                 piggyBac- 
                 2445005 
               
               
                   
                 ac- 
                 ac 
                 
                   tropicalis 
                 
                 9 
                   
                 1_XT:1-14 
                 1_XT:6156- 
                 1_XT:1579- 
                 TAG 
                   
                   
                   
                 1_XT:1- 
                 1_XT:337 
                 _L97 
               
               
                   
                 1_XT 
                   
                   
                   
                   
                   
                 6169 
                 3372 
                   
                   
                   
                   
                 1578 
                 3-6169 
                   
               
               
                   
               
               
                 98 
                 Tigger 
                 TcMari 
                 
                   T. 
                 
                 2,41 
                  8.50% 
                 Tiggerl:1- 
                 Tiggerl:240 
                 Tiggerl:425 
                 CTAAT 
                 353 
                 244 
                 597 
                 Tiggerl:1- 
                 Tiggerl:1 
                 2445005 
               
               
                   
                 1 
                 ner 
                 
                   manatus 
                 
                 8 
                   
                 13 
                 6-2418 
                 -1789 
                 TAG 
                   
                   
                   
                 424 
                 790-2418 
                 _L98 
               
               
                   
               
               
                 99 
                 Mari 
                 TcMari 
                 
                   mississip 
                 
                 2,88 
                  8.80% 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TTTAT 
                 2 
                 290 
                 292 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 ner- 
                 ner 
                 
                   piensis 
                 
                 1 
                   
                 1_Crp:1-13 
                 1_Crp:2869- 
                 1_Crp:823- 
                 AAT 
                   
                   
                   
                 1_Crp:1- 
                 1_Crp:261 
                 _L99 
               
               
                   
                 1_Crp 
                   
                   
                   
                   
                   
                 2881 
                 2613 
                   
                   
                   
                   
                 822 
                 4-2881 
                   
               
               
                   
               
               
                 101 
                 S_DM 
                 TcMari 
                 
                   D. 
                 
                 1,73 
                  9.10% 
                 S_DM:1- 
                 S_DM:1503 
                 S_DM:404- 
                 CTATA 
                 34 
                 12 
                 46 
                 S_DM:1- 
                 S_DM:14 
                 2445005 
               
               
                   
                   
                 ner 
                 
                   meianoga 
                 
                 6 
                   
                 234 
                 -1736 
                 1441 
                 TAG 
                   
                   
                   
                 403 
                 42-1736 
                 _L101 
               
               
                   
                   
                   
                 
                   ster 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 102 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 2,76 
                  9.20% 
                 hAT- 
                 hAT- 
                 hAT- 
                 CTCAT 
                 0 
                 1888 
                 1888 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 12_Crp 
                   
                 
                   mississip 
                 
                 1 
                   
                 12_Crp:1-16 
                 12_Crp:274 
                 12_Crp:67- 
                 AAA 
                   
                   
                   
                 12_Crp:1- 
                 12_Crp:22 
                 _L102 
               
               
                   
                   
                   
                 
                   piensis 
                 
                   
                   
                   
                 6-2761 
                 2262 
                   
                   
                   
                   
                 66 
                 63-2761 
                   
               
               
                   
               
               
                 103 
                 hAT- 
                 hAT- 
                 
                   P. 
                 
                 2311 
                  9.40% 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 292 
                 50 
                 342 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 1_PM 
                 Charlie 
                 
                   marinus 
                 
                   
                   
                 NO: 83 
                 NO: 95 
                 NO: 107 
                 NO: 203 
                   
                   
                   
                 NO: 171 
                 NO: 183 
                   
               
               
                   
               
               
                 104 
                 Sen 
                 PIF- 
                 
                   T 
                 
                 2,22 
                  9.60% 
                 Senkushal:1 
                 Senkusha1:2 
                 Senkusha1:7 
                 TATAA 
                 0 
                 84 
                 84 
                 Senkusha1 
                 Senkusha1 
                 2445005 
               
               
                   
                 kushal 
                 Harbin 
                 
                   rubripes 
                 
                 4 
                   
                 -18 
                 207-2224 
                 88-1708 
                 GTCA 
                   
                   
                   
                 :1-787 
                 :1709- 
                 _L104 
               
               
                   
                   
                 ger 
                   
                   
                   
                   
                   
                   
                 G 
                   
                   
                   
                   
                 2224 
                   
               
               
                   
               
               
                 105 
                 Tc1- 
                 TcMari 
                 
                   P. 
                 
                 1,61 
                  9.60% 
                 Tc1- 
                 Tc1- 
                 Tc1- 
                 TATAG 
                 4 
                 19 
                 23 
                 Tc1- 
                 Tc1- 
                 2445005 
               
               
                   
                 2_PM 
                 ner 
                 
                   marinus 
                 
                 0 
                   
                 2_PM:1-28 
                 2_PM:1583- 
                 2_PM:461- 
                 TTCAC 
                   
                   
                   
                 2_PM:1- 
                 2_PM:139 
                 _L105 
               
               
                   
                   
                   
                   
                   
                   
                   
                 1610 
                 1390 
                   
                   
                   
                   
                 460 
                 1-1610 
                   
               
               
                   
               
               
                 106 
                 Harbin 
                 PIF- 
                 
                   A. 
                 
                 4,38 
                  9.70% 
                 N/A 
                 N/A 
                 Harbinger- 
                 ATATA 
                 3 
                 7 
                 10 
                 Harbinger- 
                 Harbinger- 
                 2445005 
               
               
                   
                 ger- 
                 Harbin 
                 
                   mississip 
                 
                 1 
                   
                   
                   
                 2_AMi:461- 
                 TAAG 
                   
                   
                   
                 2_AMi:1- 
                 2 AMi:18 
                 _L106 
               
               
                   
                 2_AMi 
                 ger 
                 
                   piensis 
                 
                   
                   
                   
                   
                 1885 
                 A 
                   
                   
                   
                 460 
                 86-4381 
                   
               
               
                   
               
               
                 108 
                 hAT- 
                 hAT 
                 
                   mississip 
                 
                 3,22 
                 10.80 
                 hAT- 
                 hAT- 
                 hAT- 
                 TAAG 
                 0 
                 59 
                 59 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 2_Gav 
                   
                 
                   piensis 
                 
                 2 
                 % 
                 2__Gav:1-16 
                 2__Gav:3207 
                 2__Gav:1044 
                 ATTTA 
                   
                   
                   
                 2_Gav:1- 
                 2__Gav:30 
                 _L108 
               
               
                   
                   
                   
                   
                   
                   
                   
                 -3222 
                 -3071 
                 TT 
                   
                   
                   
                 1043 
                 72-3222 
                   
               
               
                   
               
               
                 110 
                 Tigger 
                 TcMari 
                 
                   B. 
                 
                 3,45 
                 11.00 
                 Tigger2M1- 
                 Tigger2f:34 
                 Tigger26:60 
                 TA 
                 7 
                 1597 
                 1604 
                 Tigget2f:1 
                 Tigget2£2 
                 2445005 
               
               
                   
                 2f 
                 ner 
                 
                   acutorost 
                 
                 5 
                 % 
                 19 
                 37-3455 
                 3-2462 
                   
                   
                   
                   
                 -602 
                 463-3455 
                 _L110 
               
               
                   
                   
                   
                 
                   rata 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 111 
                 Tc1- 
                 TcMari 
                   D.   rerio   
                 1,57 
                 11.00 
                 Tc1- 
                 Tc1- 
                 Tc1- 
                 TA 
                 36 
                 309 
                 345 
                 Tc1- 
                 Tc1- 
                 2445005 
               
               
                   
                 4_DR 
                 ner 
                   
                 2 
                 % 
                 4_DR:1-51 
                 4_DR:1522- 
                 4_DR:296- 
                   
                   
                   
                   
                 4_DR:1- 
                 4_DR:137 
                 _L111 
               
               
                   
                   
                   
                   
                   
                   
                   
                 1572 
                 1372 
                   
                   
                   
                   
                 295 
                 3-1572 
                   
               
               
                   
               
               
                 116 
                 Tigger 
                 TcMari 
                 
                   B. 
                 
                 2,67 
                 11.90 
                 Tiggerl7:1- 
                 Tiggerl7:26 
                 Tigger17:11 
                 TA 
                 1 
                 273 
                 274 
                 Tiggerl7: 
                 Tiggerl7: 
                 2445005 
               
               
                   
                 17 
                 ner 
                 
                   acutorost 
                 
                 3 
                 % 
                 23 
                 51-2673 
                 3-2095 
                   
                   
                   
                   
                 1-112 
                 2096-2673 
                 _L116 
               
               
                   
                   
                   
                 
                   rata 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 117 
                 Tigger 
                 TcMari 
                 
                   H. 
                 
                 3,02 
                 11.90 
                 Tigger3:1- 
                 Tigger3:300 
                 Tigger3:463 
                 CTATA 
                 1 
                 1489 
                 1490 
                 Tigger3:1- 
                 Tigger3:2 
                 2445005 
               
               
                   
                 3 
                 ner 
                 
                   sapiens 
                 
                 9 
                 % 
                 23 
                 7-3029 
                 -2307 
                 TAG 
                   
                   
                   
                 462 
                 308-3029 
                 _L117 
               
               
                   
               
               
                 118 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 2,02 
                 12.30 
                 hAT- 
                 hAT- 
                 hAT- 
                 TA 
                 3 
                 1 
                 4 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 M_Crp 
                   
                 
                   mississip 
                 
                 3 
                 % 
                 14_Crp:1-2 
                 14_Crp:202 
                 14_Crp:193- 
                   
                   
                   
                   
                 14_Crp:1- 
                 14_Crp:17 
                 _L118 
               
               
                   
                   
                   
                 
                   piensis 
                 
                   
                   
                   
                 2-2023 
                 1779 
                   
                   
                   
                   
                 192 
                 80-2023 
                   
               
               
                   
               
               
                 119 
                 Marine 
                 TcMari 
                 
                   A. 
                 
                 1,98 
                 12.30 
                 Mariner- 
                 Mariner- 
                 Mariner- 
                 TTAA 
                 n 
                 25 
                 27 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r- 
                 ner 
                 
                   mississip 
                 
                 4 
                 % 
                 9_Crp:1-19 
                 9_Crp:1966- 
                 9_Crp:252- 
                   
                   
                   
                   
                 9_Crp:1- 
                 9_Crp:156 
                 _L119 
               
               
                   
                 9_Crp 
                   
                 
                   piensis 
                 
                   
                   
                   
                 1984 
                 1559 
                   
                   
                   
                   
                 251 
                 0-1984 
                   
               
               
                   
               
               
                 124 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 5,87 
                 12.80 
                 hAT- 
                 hAT- 
                 hAT- 
                 TA 
                 0 
                 566 
                 566 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 11_AM 
                   
                 
                   mississip 
                 
                 1 
                 % 
                 11_AMi:1- 
                 11_AMi:585 
                 11_AMi:879 
                   
                   
                   
                   
                 11_AMi:1 
                 11_AMi:3 
                 _L124 
               
               
                   
                 i 
                   
                 
                   piensis 
                 
                   
                   
                 16 
                 6-5871 
                 -3152 
                   
                   
                   
                   
                 -878 
                 153-5871 
                   
               
               
                   
               
               
                 126 
                 Marine 
                 TcMari 
                 
                   A. 
                 
                 2,67 
                 13.00 
                 N/A 
                 N/A 
                 Mariner- 
                 TA 
                 2 
                 6 
                 8 
                 Mariner- 
                 Mariner- 
                 2445005 
               
               
                   
                 r- 
                 ner 
                 
                   mississip 
                 
                 1 
                 % 
                   
                   
                 10_Crp:439- 
                   
                   
                   
                 10_Crp:1- 
                 10 Op:15 
                 _L126 
                 _L126 
               
               
                   
                 10_Crp 
                   
                 
                   piensis 
                 
                   
                   
                   
                   
                 1527 
                   
                   
                   
                 438 
                 28-2671 
                   
                   
               
               
                   
               
               
                 139 
                 hAT- 
                 hAT-Ac 
                 
                   A. 
                 
                 5670 
                 13.80 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 1 
                 302.2 
                 3023 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 17_Cro 
                   
                 
                   mississip 
                 
                   
                 % 
                 NO: 84 
                 NO: 96 
                 NO: 108 
                 NO: 196 
                   
                   
                   
                 NO: 172 
                 NO: 184 
                   
               
               
                   
                 c 
                   
                 
                   piensis 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 140 
                 hAT- 
                 hAT 
                 
                   mississip 
                 
                 5,52 
                 13.80 
                 hAT- 
                 hAT- 
                 hAT- 
                 TA 
                 1 
                 500 
                 501 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 4_AMi 
                   
                 
                   piensis 
                 
                 1 
                 % 
                 4_AMi:1-14 
                 4_AMi:5508 
                 4_AMi:1477 
                   
                   
                   
                   
                 4_AMi:1- 
                 4_AMi:37 
                 _L140 
               
               
                   
                   
                   
                   
                   
                   
                   
                 -5521 
                 -3756 
                   
                   
                   
                   
                 1476 
                 57-5521 
                   
               
               
                   
               
               
                 142 
                 Tigger 
                 TcMar- 
                 
                   M.murinu 
                 
                 2731 
                 13.80 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 15 
                 1430 
                 1445 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 4 
                 Tigger 
                 
                   s 
                 
                   
                 % 
                 NO: 85 
                 NO: 97 
                 NO: 109 
                 NO: 193 
                   
                   
                   
                 NO: 173 
                 NO: 185 
                   
               
               
                   
               
               
                 144 
                 Tigger 
                 TcMar- 
                 
                   T 
                 
                 2492 
                 13.80 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 2 
                 313 
                 315 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 7 
                 Tigger 
                 
                   manatus 
                 
                   
                 % 
                 NO: 86 
                 NO: 98 
                 NO: 110 
                 NO: 193 
                   
                   
                   
                 NO: 174 
                 NO: 186 
                   
               
               
                   
                   
                   
                 
                   latirostris 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 156 
                 Tigger 
                 TcMari 
                 
                   P. 
                 
                 2,71 
                 14.20 
                 Tigger:1- 
                 Tigger2:270 
                 Tigger2:603 
                 CTATA 
                 27 
                 1155 
                 1182 
                 Tigger2:1- 
                 Tigger2:2 
                 2445005 
               
               
                   
                 2 
                 ner 
                 
                   paniscus 
                 
                 8 
                 % 
                 19 
                 0-2718 
                 -2486 
                 TAG 
                   
                   
                   
                 602 
                 487-2718 
                 _L156 
               
               
                   
               
               
                 180 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 2844 
                 14.50 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 0 
                 9 
                 9 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 19J7rp 
                   
                 
                   mississip 
                 
                   
                 % 
                 NO: 87 
                 NO: 99 
                 NO: 111 
                 NO: 204 
                   
                   
                   
                 NO: 175 
                 NO: 187 
                   
               
               
                   
                   
                   
                 
                   piensis 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 183 
                 hAT- 
                 hAT-Ac 
                 
                   A. 
                 
                 6042 
                 14.60 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 0 
                 2162 
                 2162 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 17B_C 
                   
                 
                   mississip 
                 
                   
                 % 
                 NO: 88 
                 NO: 100 
                 NO: 112 
                 NO: 205 
                   
                   
                   
                 NO: 176 
                 NO: 188 
                   
               
               
                   
                 roc 
                   
                 
                   piensis 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 188 
                 Tc1- 
                 TcMari 
                 
                   x 
                 
                 1,62 
                 14.60 
                 Tc1- 
                 Tc1- 
                 Tc1- 
                 TATTT 
                 0 
                 7 
                 7 
                 Tc1- 
                 Tei- 
                 2445005 
               
               
                   
                 13Xt 
                 ner 
                 
                   tropicalis 
                 
                 4 
                 % 
                 13_Xt:1-27 
                 13_Xt:1598- 
                 13_Xt:381- 
                 ATA 
                   
                   
                   
                 13_Xt:1- 
                 13_Xt:140 
                 _L188 
               
               
                   
                   
                   
                   
                   
                   
                   
                 1624 
                 1403 
                   
                   
                   
                   
                 380 
                 4-1624 
                   
               
               
                   
               
               
                 197 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 2,51 
                 15.00 
                 hAT- 
                 hAT- 
                 hAT- 
                 TA 
                 11 
                 17 
                 28 
                 hAT- 
                 hAT- 
                 2445005 
               
               
                   
                 1913_C 
                   
                 
                   mississip 
                 
                 7 
                 % 
                 19B_Croc:1 
                 19B_Croc:2 
                 19B_Croc:3 
                   
                   
                   
                   
                 19B_Croc: 
                 19B_Croc: 
                 _L197 
               
               
                   
                 roc 
                   
                 
                   piensis 
                 
                   
                   
                 -15 
                 503-2517 
                 50-2095 
                   
                   
                   
                   
                 1-349 
                 2096-2517 
                   
               
               
                   
               
               
                 199 
                 MarsTi 
                 TcMari 
                 
                   M. 
                 
                 2,25 
                 15.00 
                 MarsTigger 
                 MarsTigger 
                 MarsTigger 
                 TTAA 
                 1 
                 71 
                 72 
                 MarsTigge 
                 MarsTigge 
                 2445005 
               
               
                   
                 gger8 
                 ner 
                 
                   eugenii 
                 
                 3 
                 % 
                 8:1-23 
                 8:2231-2253 
                 8:322-1950 
                   
                   
                   
                   
                 1-8:1-321 
                 r8:1951- 
                 _L199 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 225.3 
                   
               
               
                   
               
               
                 212 
                 Tigger 
                 TcMari 
                 
                   C. 
                 
                 2,40 
                 15.60 
                 Tigger5:1- 
                 Tigger5:238 
                 Tigger5:152 
                 TA 
                 1 
                 124 
                 12.5 
                 Tigger5:1- 
                 Tigger5:2 
                 2445005 
               
               
                   
                 5 
                 ner 
                 
                   familiaris 
                 
                 6 
                 % 
                 24 
                 3-2406 
                 -2068 
                   
                   
                   
                   
                 151 
                 069-2406 
                 _L212 
               
               
                   
               
               
                 222 
                 hAT- 
                 hAT-Ac 
                 
                   X. 
                 
                 1114 
                 16.00 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 0 
                 141 
                 141 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 10_XT 
                   
                 
                   tropicalis 
                 
                 4 
                 % 
                 NO: 89 
                 NO: 101 
                 NO: 113 
                 NO: 206 
                   
                   
                   
                 NO: 177 
                 NO: 189 
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 237 
                 hAT- 
                 hAT 
                 
                   A. 
                 
                 5,16 
                 16.60 
                 hAT- 
                 hAT- 
                   
                   
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 6_AM 
                   
                 
                   mississip 
                 
                 2 
                 % 
                 6_AMi:1-15 
                 6_AMi:5148 
                 6_AMi:2157 
                   
                   
                   
                   
                 6_AMi:1- 
                 6_AMi:33 
                 2445005 
               
               
                   
                   
                   
                 
                   piensis 
                 
                   
                   
                   
                 -5162 
                 -3356 
                   
                   
                   
                   
                 2156 
                 57-5162 
                 _L237 
               
               
                   
               
               
                 245 
                 Mars 
                 TcMari 
                 
                   M. 
                 
                 1,81 
                 17.30 
                 MarsFigger 
                 MarsTigger 
                 MarsTigger 
                 TA 
                 1 
                 319 
                 320 
                 MarsTi gge 
                 MarsTigge 
                 2445005 
               
               
                   
                 Tigger 
                 ner 
                 
                   domestica 
                 
                 3 
                 % 
                 lc:1-22 
                 lc:1792- 
                 lc:503-1813 
                   
                   
                   
                   
                 rlc:1-502 
                 rlc:1814- 
                 _L245 
               
               
                   
                 1c 
                   
                   
                   
                   
                   
                 1813 
                   
                   
                   
                   
                   
                   
                 1813 
                   
               
               
                   
               
               
                 246 
                 Tigger 
                 TcMari 
                 
                   B. 
                 
                 1,18 
                 17.40 
                 Tigger17c:1 
                 Tiggerl7c:1 
                 Tigger17c:1 
                 TA 
                 3 
                 268 
                 271 
                 Tiggerl7c: 
                 Tigger17c: 
                 2445005 
               
               
                   
                 17c 
                 ner 
                 
                   acutorost 
                 
                 8 
                 % 
                 -23 
                 166-1188 
                 13-982 
                   
                   
                   
                   
                 1-112 
                 983-1188 
                 _L246 
               
               
                   
                   
                   
                 
                   rata 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 258 
                 Harbin 
                 P1F- 
                 
                   A 
                 
                 6,56 
                 17.90 
                 N/A 
                 N/A 
                 Harbinger- 
                 CTCTA 
                 0 
                 29 
                 29 
                 Harbinger- 
                 Harbinger- 
                 2445005 
               
               
                   
                 ger- 
                 Harbin 
                 
                   mississip 
                 
                 8 
                 % 
                   
                   
                 1_AMi:4005 
                 GAG 
                   
                   
                   
                 i amli- 
                 1_AMi:54 
                 _L258 
               
               
                   
                 1_AM 
                 ger 
                 
                   piensis 
                 
                   
                   
                   
                   
                 -5459 
                   
                   
                   
                   
                 4004 
                 60-6568 
                   
               
               
                   
               
               
                 260 
                 Tc1- 
                 TcMari 
                 
                   X. 
                 
                 1,61 
                 18.10 
                 Tc1- 
                 Tc1- 
                 Tc1- 
                 TA 
                 n 
                 75 
                 77 
                 Tc1- 
                 Tc1- 
                 2445005 
               
               
                   
                 14_Xt 
                 ner 
                 
                   tropiealis 
                 
                 2 
                 % 
                 14_Xt:1-214 
                 14_Xt:1400- 
                 14_Xt:362- 
                   
                   
                   
                   
                 14_Xt:1- 
                 14_Xt:139 
                 _L260 
               
               
                   
                   
                   
                   
                   
                   
                   
                 1612 
                 1393 
                   
                   
                   
                   
                 361 
                 4-1612 
                   
               
               
                   
               
               
                 271 
                 Kanga 
                 TcMari 
                   V.   pacos   
                 1,74 
                 18.80 
                 Kangal:1- 
                 Kangal:172 
                 Kanga1:240 
                 CTATA 
                 0 
                 7 
                 7 
                 Kangal:1- 
                 Kangal:15 
                 2445005 
               
               
                   
                 r 
                 ner 
                   
                 5 
                 % 
                 22 
                 4-1745 
                 -1511 
                 TAG 
                   
                   
                   
                 239 
                 12-1745 
                 _L271 
               
               
                   
               
               
                 275 
                 Arthur 
                 hAT 
                 
                   G. 
                 
                 3,94 
                 19.20 
                 Arthur1:1- 
                 Arthurl:393 
                 Arthur1:115 
                 TA 
                 0 
                 32 
                 32 
                 Arthur1:1- 
                 Arthurl:3 
                 2445005 
               
               
                   
                 1 
                   
                 
                   variegatus 
                 
                 7 
                 % 
                 11 
                 7-3947 
                 0-3711 
                   
                   
                   
                   
                 1149 
                 712-3947 
                 _L275 
               
               
                   
               
               
                 295 
                 Harbin 
                 PIF- 
                 
                   mississip 
                 
                 5,17 
                 20.00 
                 Harbinger- 
                 Harbinger- 
                 Harbinger- 
                 TA 
                 1 
                 44 
                 45 
                 Harbinger- 
                 Harbinger- 
                 2445005 
               
               
                   
                 ger- 
                 Harbin 
                 
                   piensis 
                 
                 2 
                 % 
                 1_Crp:1-21 
                 1_Crp:5152- 
                 1_Crp:576- 
                   
                   
                   
                   
                 1_Crp:1- 
                 1_Crp:237 
                 _L295 
               
               
                   
                 1_Crp 
                 ger 
                   
                   
                   
                   
                 5172. 
                 2375 
                   
                   
                   
                   
                 575 
                 6-5172 
                   
               
               
                   
               
               
                 314 
                 Zaphod 
                 hAT 
                 
                   B. 
                 
                 2,62 
                 21.00 
                 Zaphod3:1- 
                 Zaphod3:26 
                 Zaphod3:87 
                 TA 
                 0 
                 1 
                 1 
                 Zaphod3:1 
                 Zaphod3:2 
                 2445005 
               
               
                   
                 3 
                   
                 
                   acutorost 
                 
                 4 
                 % 
                 12 
                 13-2624 
                 8-2296 
                   
                   
                   
                   
                 -877 
                 297-2624 
                 _L314 
               
               
                   
                   
                   
                 
                   rata 
                 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 320 
                 Joey1 
                 TcMari 
                 
                   M.  
                 
                 1,36 
                 21.20 
                 Joeyl:1-27 
                 Joeyl:1339- 
                 Joey1:255- 
                 TA 
                 0 
                 5 
                 5 
                 Joey 1 1- 
                 Joeyl:127 
                 2445005 
               
               
                   
                   
                 ner 
                 
                   eugenii 
                 
                 5 
                 % 
                   
                 1365 
                 1271 
                   
                   
                   
                   
                 254 
                 2-1365 
                 _L320 
               
               
                   
               
               
                 331 
                 Zaphod 
                 hAT 
                 
                   c. 
                 
                 4,07 
                 21.90 
                 Zaphod:1-11 
                 Zaphod:406 
                 Zaphod:101 
                 TTAA 
                 0 
                 0 
                 0 
                 Zaphod:1- 
                 Zaphod:37 
                 2445005 
               
               
                   
                 3 
                   
                 
                   syrichta 
                 
                 9 
                 % 
                   
                 9-4079 
                 0-3745 
                   
                   
                   
                   
                 1009 
                 46-4079 
                 _L331 
               
               
                   
               
               
                 342 
                 Harbin 
                 PIF- 
                 
                   mississip 
                 
                 5,32 
                 23.10 
                 Harbinger- 
                 Harbinger- 
                 Harbinger- 
                 TTAA 
                 1 
                 16 
                 17 
                 Harbinger- 
                 Harbinger- 
                 2445005 
               
               
                   
                 ger- 
                 Harbin 
                 
                   piensis 
                 
                 9 
                 % 
                 3_AMi:1-21 
                 3_AMi:5309 
                 3_AMi:343- 
                   
                   
                   
                   
                 3_AMi1- 
                 3 AMr:20 
                 _L312 
               
               
                   
                 3_AMi 
                 ger 
                   
                   
                   
                   
                 -5329 
                 2007 
                   
                   
                   
                   
                 342 
                 08-5329 
                   
               
               
                   
               
               
                 348 
                 Harbin 
                 PIF- 
                 
                   A. 
                 
                 5,233 
                 23.90 
                 N/A 
                 N/A 
                 Harbinger- 
                 TA 
                 0 
                 16 
                 16 
                 Harbinger- 
                 Harbinger- 
                 2445005 
               
               
                   
                 ger- 
                 Harbin 
                 
                   mississip 
                 
                   
                 % 
                   
                   
                 IB_Crp:311 
                   
                   
                   
                   
                 IB_Crp:1- 
                 1B_Crp:1 
                 _L348 
               
               
                   
                 IB_ 
                 ger 
                 
                   piensis 
                 
                   
                   
                   
                   
                 -1342 
                   
                   
                   
                   
                 310 
                 343-5233 
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 CrP 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 349 
                 MARW 
                 TcMar- 
                   D.   yakuba   
                 1643 
                 24.10 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 SEQ ID 
                 0 
                 0 
                 0 
                 SEQ ID 
                 SEQ ID 
                   
               
               
                   
                 OLENI 
                 Tcl 
                   
                   
                 % 
                 NO: 90 
                 NO: 102 
                 NO: 114 
                 NO: 193 
                   
                   
                   
                 NO: 178 
                 NO: 190 
                   
               
               
                   
               
               
                 351 
                 Zaphod 
                 hAT 
                 
                   G. 
                 
                 3,592 
                 24.40 
                 Zaphod2:1-9 
                 Zaphod2:35 
                 Zaphod2:82 
                 TA 
                 0 
                 0 
                 0 
                 Zaphod2:1 
                 Zaphod2.:3 
                 2445005 
               
               
                   
                 2 
                   
                 
                   variegatu 
                 
                   
                 % 
                   
                 84-3592 
                 2-3356 
                   
                   
                   
                   
                 ′-821 
                 357-3592 
                 _L351 
               
               
                   
                   
                   
                 
                   8 
                 
               
               
                   
               
               
                 * Except for where SEQ ID NOs are indicated in this table for 5′ TR, 3′ TR, transposase, LTF and RTF, the sequences of these corresponding elements are annotated as the locations of the nucleotides in the corresponding TE sequence as identified by the Repbase ID, the sequences of which are incorporated herein by reference. The Repbase database can be accessed at world wide web.girinst.org. The sequences of the TEs can also be accessed at NCBI (world wide web. ncbi.nlm.nih.gov) with the temporary NCBI identifier in the last column. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Active transposable elements (TEs) as validated by the transposition assays. 
               
            
           
           
               
               
               
               
            
               
                   
                 SEQ ID NOS 
                 Transfer 
                 Transfer 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 TE 
                   
                   
                   
                 Length 
                   
                   
                   
                 Trans- 
                   
                   
                 5′TSD, 
                 efficiency 
                 efficiency 
               
               
                 ID 
                 TE name 
                 Superfamily 
                 Species 
                 (bp) 
                 Divergence 
                 5′TR 
                 3′TR 
                 posase 
                 LTF 
                 RTF 
                 3′TSD 
                 in 293T 
                 in HeLa 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 1 
                 hAT-2_AG 
                 hAT 
                 
                   A. gambiae 
                 
                 4,803 
                 0.00% 
                 1 
                 27 
                 53 
                 115 
                 141 
                 191 
                 5.19% 
                 7.54% 
               
               
                 9 
                 HAT1_AG 
                 hAT 
                 
                   A. gambiae 
                 
                 3,702 
                 0.10% 
                 2 
                 28 
                 54 
                 116 
                 142 
                 192 
                 5.28% 
                 0.87% 
               
               
                 14 
                 Tc1-8B_DR 
                 TcMariner 
                 
                   D. rerio 
                 
                 1,597 
                 0.20% 
                 3 
                 29 
                 55 
                 117 
                 143 
                 193 
                   *15.19 % 
                   *11.81 % 
               
               
                 17 
                 hAT-6_PM 
                 hAT 
                 
                   P. marinus 
                 
                 2,391 
                 0.20% 
                 4 
                 30 
                 56 
                 118 
                 144 
                 195 
                 3.16% 
                 1.38% 
               
               
                 22 
                 hAT-3_XT 
                 hAT 
                 
                   X. tropicalis 
                 
                 2,922 
                 0.30% 
                 5 
                 31 
                 57 
                 119 
                 145 
                 196 
                 0.27% 
                 0.87% 
               
               
                 25 
                 Tc1-3_Xt 
                 TcMariner 
                 
                   X. tropicalis 
                 
                 1,614 
                 0.40% 
                 6 
                 32 
                 58 
                 120 
                 146 
                 193 
                 9.62% 
                 3.69% 
               
               
                 28 
                 hAT-5_DR 
                 hAT 
                 
                   D. rerio 
                 
                 2,496 
                 0.60% 
                 7 
                 33 
                 59 
                 121 
                 147 
                 194 
                 2.01% 
                 0.05% 
               
               
                 29 
                 Tc1-3_FR 
                 TcMariner 
                 
                   T. rubripes 
                 
                 1,595 
                 0.70% 
                 8 
                 34 
                 60 
                 122 
                 148 
                 193 
                   *19.99 % 
                 10.61% 
               
               
                 32 
                 Tc1-5_Xt 
                 TcMariner 
                 
                   X. tropicalis 
                 
                 1,594 
                 1.10% 
                 9 
                 35 
                 61 
                 123 
                 149 
                 193 
                 7.55% 
                 6.30% 
               
               
                 33 
                 Tc1-10_Xt 
                 TcMar-Tc1 
                 
                   X. tropicalis 
                 
                 1606 
                 1.30% 
                 10 
                 36 
                 62 
                 124 
                 150 
                 193 
                 1.64% 
                 2.43% 
               
               
                 35 
                 Mariner2_AG 
                 TcMariner 
                 
                   A. gambiae 
                 
                 2,000 
                 1.50% 
                 11 
                 37 
                 63 
                 125 
                 151 
                 193 
                   *17.28 % 
                   *9.39 % 
               
               
                 36 
                 Tc1-1_Xt 
                 TcMariner 
                 
                   X. laevis 
                 
                 1,634 
                 1.50% 
                 12 
                 38 
                 64 
                 126 
                 152 
                 193 
                   *21.95 % 
                   *9.98 % 
               
               
                 37 
                 Tc1-1_AG 
                 TcMariner 
                 
                   A. gambiae 
                 
                 1,413 
                 1.60% 
                 13 
                 39 
                 65 
                 127 
                 153 
                 193 
                 11.18% 
                 6.43% 
               
               
                 39 
                 Tc1DR3_Xt 
                 TcMariner 
                 
                   X. tropicalis 
                 
                 1,231 
                 1.70% 
                 14 
                 40 
                 66 
                 128 
                 154 
                 198 
                 1.26% 
                 3.12% 
               
               
                 41 
                 hAT-3_PM 
                 hAT 
                 
                   P. marinus 
                 
                 2,984 
                 1.90% 
                 15 
                 41 
                 67 
                 129 
                 155 
                 199 
                 3.47% 
                 0.33% 
               
               
                 43 
                 Tc1-1_PM 
                 TcMariner 
                 
                   P. marinus 
                 
                 1,607 
                 2.00% 
                 16 
                 42 
                 68 
                 130 
                 156 
                 193 
                 13.56% 
                 8.97% 
               
               
                 45 
                 Mariner- 
                 TcMariner 
                 
                   A. 
                 
                 2,121 
                 2.10% 
                 17 
                 43 
                 69 
                 131 
                 157 
                 193 
                 6.79% 
                 1.96% 
               
               
                   
                 4_AMi 
                   
                 
                   mississippiensis 
                 
               
               
                 46 
                 Myotis_hAT1 
                 hAT 
                 
                   M. lucifugus 
                 
                 2,921 
                 2.10% 
                 18 
                 44 
                 70 
                 132 
                 158 
                 197 
                 6.99% 
                 2.89% 
               
               
                 47 
                 hAT-7_PM 
                 hAT 
                 
                   P. marinus 
                 
                 2,499 
                 2.20% 
                 19 
                 45 
                 71 
                 133 
                 159 
                 200 
                 7.40% 
                 4.34% 
               
               
                 51 
                 Tc1-11_Xt 
                 TcMariner 
                 
                   X. tropicalis 
                 
                 1633 
                 2.50% 
                 20 
                 46 
                 72 
                 134 
                 160 
                 193 
                 4.45% 
                 1.96% 
               
               
                 52 
                 Tc1-16_Xt 
                 TcMariner 
                 
                   X. tropicalis 
                 
                 1635 
                 2.80% 
                 21 
                 47 
                 73 
                 135 
                 161 
                 193 
                 9.88% 
                 7.87% 
               
               
                 54 
                 Tc1-4_Xt 
                 TcMariner 
                 
                   X. tropicalis 
                 
                 1596 
                 3.30% 
                 22 
                 48 
                 74 
                 136 
                 162 
                 193 
                 14.79% 
                   *6.80 % 
               
               
                 56 
                 Tc1-15_Xt 
                 TcMariner 
                 
                   X. tropicalis 
                 
                 1581 
                 3.50% 
                 23 
                 49 
                 75 
                 137 
                 163 
                 193 
                 13.76% 
                 3.84% 
               
               
                 59 
                 TC1_FR2 
                 TcMariner 
                 
                   T. rubripes 
                 
                 1602 
                 3.80% 
                 24 
                 50 
                 76 
                 138 
                 164 
                 193 
                 5.86% 
                 1.08% 
               
               
                 63 
                 hAT-9_XT 
                 hAT 
                 
                   X. tropicalis 
                 
                 5716 
                 4.60% 
                 25 
                 51 
                 77 
                 139 
                 165 
                 201 
                 5.63% 
                 0.99% 
               
               
                 64 
                 Mariner- 
                 TcMariner 
                 
                   X. tropicalis 
                 
                 1840 
                 4.60% 
                 26 
                 52 
                 78 
                 140 
                 166 
                 193 
                 8.56% 
                 3.13% 
               
               
                   
                 5_XT 
               
               
                 70 
                 Mariner- 
                 TcMar-Tc2 
                 
                   A. 
                 
                 2479 
                 5.10% 
                 79 
                 91 
                 103 
                 167 
                 179 
                 193 
                 10.27% 
                 N/A 
               
               
                   
                 6_AMi 
                   
                 
                   mississippiensis 
                 
               
               
                 82 
                 piggyBac- 
                 PiggyBac 
                 
                   X. tropicalis 
                 
                 8451 
                 6.70% 
                 80 
                 92 
                 104 
                 168 
                 180 
                 202 
                 3.55% 
                 N/A 
               
               
                   
                 2_XT 
               
               
                 86 
                 piggyBac- 
                 PiggyBac 
                 
                   A. 
                 
                 3224 
                 7.00% 
                 81 
                 93 
                 105 
                 169 
                 181 
                 202 
                 7.36% 
                 N/A 
               
               
                   
                 1_AMi 
                   
                 
                   mississippiensis 
                 
               
               
                 90 
                 Mariner- 
                 TcMar- 
                 
                   A. 
                 
                 2292 
                 7.50% 
                 82 
                 94 
                 106 
                 170 
                 182 
                 193 
                 10.58% 
                 N/A 
               
               
                   
                 3_Crp 
                 Tigger 
                 
                   mississippiensis 
                 
               
               
                 103 
                 hAT-1_PM 
                 hAT- 
                 
                   P. marinus 
                 
                 2311 
                 9.40% 
                 83 
                 95 
                 107 
                 171 
                 183 
                 203 
                 2.51% 
                 N/A 
               
               
                   
                   
                 Charlie 
               
               
                 139 
                 hAT-17_Croc 
                 hAT-Ac 
                 
                   A. 
                 
                 5670 
                 13.80% 
                 84 
                 96 
                 108 
                 172 
                 184 
                 196 
                 3.63% 
                 4.73% 
               
               
                   
                   
                   
                 
                   mississippiensis 
                 
               
               
                 142 
                 Tigger4 
                 TcMar- 
                 
                   M. murinus 
                 
                 2731 
                 13.80% 
                 85 
                 97 
                 109 
                 173 
                 185 
                 193 
                 6.17% 
                 2.28% 
               
               
                   
                   
                 Tigger 
               
               
                 144 
                 Tigger7 
                 TcMar- 
                 
                   T. manatus 
                 
                 2492 
                 13.80% 
                 86 
                 98 
                 110 
                 174 
                 186 
                 193 
                 1.90% 
                 N/A 
               
               
                   
                   
                 Tigger 
                 
                   latirostris 
                 
               
               
                 180 
                 hAT-19_Crp 
                 hAT 
                 
                   A. 
                 
                 2844 
                 14.50% 
                 87 
                 99 
                 111 
                 175 
                 187 
                 204 
                 1.23% 
                 N/A 
               
               
                   
                   
                   
                 
                   mississippiensis 
                 
               
               
                 183 
                 hAT- 
                 hAT-Ac 
                 
                   A. 
                 
                 6042 
                 14.60% 
                 88 
                 100 
                 112 
                 176 
                 188 
                 205 
                 0.77% 
                 N/A 
               
               
                   
                 17B_Croc 
                   
                 
                   mississippiensis 
                 
               
               
                 222 
                 hAT-10_XT 
                 hAT-Ac 
                 
                   X. tropicalis 
                 
                 11144 
                 16.00% 
                 89 
                 101 
                 113 
                 177 
                 189 
                 206 
                 5.07% 
                 N/A 
               
               
                 349 
                 MARWOLEN1 
                 TcMar-Tc1 
                 
                   D. yakuba 
                 
                 1643 
                 24.10% 
                 90 
                 102 
                 114 
                 178 
                 190 
                 193 
                 6.31% 
                 N/A 
               
               
                   
               
               
                 The * before a transfer efficiency indicates an underestimation of the real value. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
               
                   
               
               
                 Sequence similarities among the 14 identified hAT 
               
               
                 superfamily transposases and control TE TcBuster 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 TcBusterA2:K17 
                 100.0% 
                   
                   
                   
                   
               
               
                 103_hAT-1_PM 
                 48.3% 
                 100.0% 
               
               
                 17_hAT-6_PM 
                 36.9% 
                 40.8% 
                 100.0% 
               
               
                 46_Myotis_hAT1 
                 35.6% 
                 39.8% 
                 48.1% 
                 100.0% 
               
               
                 28_hAT-5_DR 
                 38.2% 
                 40.6% 
                 45.7% 
                 43.6% 
                 100.0% 
               
               
                 22_hAT-3_XT 
                 39.0% 
                 39.3% 
                 42.7% 
                 41.1% 
                 47.8% 
               
               
                 41_hAT-3_PM 
                 39.6% 
                 38.5% 
                 42.3% 
                 42.5% 
                 47.9% 
               
               
                 47_hAT-7_PM 
                 40.1% 
                 37.8% 
                 43.3% 
                 44.6% 
                 49.9% 
               
               
                 180_hAT-19_Crp 
                 17.7% 
                 18.5% 
                 18.9% 
                 19.7% 
                 20.6% 
               
               
                 1_hAT-2_AG 
                 23.0% 
                 23.3% 
                 22.3% 
                 24.3% 
                 25.8% 
               
               
                 9_HAT1_AG 
                 21.0% 
                 19.2% 
                 22.4% 
                 23.7% 
                 23.2% 
               
               
                 139_hAT-17_Croc 
                 22.6% 
                 20.1% 
                 18.6% 
                 21.1% 
                 23.2% 
               
               
                 183_hAT-17B_Croc 
                 22.0% 
                 19.6% 
                 18.7% 
                 20.8% 
                 22.5% 
               
               
                 63_hAT-9_XT 
                 25.0% 
                 22.9% 
                 20.3% 
                 22.7% 
                 24.3% 
               
               
                 222_hAT-10_XT 
                 22.9% 
                 21.1% 
                 20.9% 
                 23.1% 
                 23.3% 
               
               
                   
                 TcBuster 
                 103_hAT- 
                 17_hAT- 
                 46_Myotis_hAT1 
                 28_hAT- 
               
               
                   
                   
                 1_PM 
                 6_PM 
                   
                 5_DR 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 TcBusterA2:K17 
                   
                   
                   
                   
                   
               
               
                   
                 103_hAT-1_PM 
               
               
                   
                 17_hAT-6_PM 
               
               
                   
                 46_Myotis_hAT1 
               
               
                   
                 28_hAT-5_DR 
               
               
                   
                 22_hAT-3_XT 
                 100.0% 
               
               
                   
                 41_hAT-3_PM 
                 51.9% 
                 100.0% 
               
               
                   
                 47_hAT-7_PM 
                 53.4% 
                 56.1% 
                 100.0% 
               
               
                   
                 180_hAT-19_Crp 
                 18.2% 
                 19.0% 
                 18.5% 
                 100.0% 
               
               
                   
                 1_hAT-2_AG 
                 22.5% 
                 24.2% 
                 23.8% 
                 24.9% 
                 100.0% 
               
               
                   
                 9_HAT1_AG 
                 22.0% 
                 22.1% 
                 22.6% 
                 24.0% 
                 62.8% 
               
               
                   
                 139_hAT-17_Croc 
                 21.9% 
                 21.2% 
                 21.7% 
                 24.5% 
                 38.8% 
               
               
                   
                 183_hAT-17B_Croc 
                 21.2% 
                 20.7% 
                 21.1% 
                 24.2% 
                 38.3% 
               
               
                   
                 63_hAT-9_XT 
                 20.2% 
                 20.9% 
                 21.6% 
                 27.6% 
                 38.9% 
               
               
                   
                 222_hAT-10_XT 
                 19.9% 
                 22.9% 
                 22.6% 
                 26.1% 
                 36.9% 
               
               
                   
                   
                 22_hAT- 
                 41_hAT- 
                 47_hAT- 
                 180_hAT- 
                 1_hAT- 
               
               
                   
                   
                 3_XT 
                 3_PM 
                 7_PM 
                 19_Crp 
                 2_AG 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
               
                   
               
               
                 Sequence similarities among the 14 identified piggyBac 
               
               
                 superfamily transposases and control TE piggyBac 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 82_piggyBac-2_XT 
                 100.0% 
                   
                   
               
               
                 piggyBac 
                 33.1% 
                 100.0% 
               
               
                 86_piggyBac-1_AMi 
                 35.8% 
                 44.5% 
                 100.0% 
               
               
                   
                 82_piggyBac-2_XT 
                 piggyBac 
                 86_piggyBac-1_AMi 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
               
                   
               
               
                 Sequence similarities among the 22 identified TcMariner superfamily transposases and control TE SB100X 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 56_Tc1-15_Xt 
                 100.0% 
                   
                   
                   
                   
                   
               
               
                 59_TC1_FR2 
                 82.5% 
                 100.0% 
               
               
                 39_Tc1DR3_Xt 
                 43.0% 
                 44.4% 
                 100.0% 
               
               
                 35_Mariner2_AG 
                 41.6% 
                 41.0% 
                 43.7% 
                 100.0% 
               
               
                 37_Tc1-1_AG 
                 39.3% 
                 41.7% 
                 40.2% 
                 55.0% 
                 100.0% 
               
               
                 32_Tc1-5_Xt 
                 44.4% 
                 43.9% 
                 45.0% 
                 44.0% 
                 40.2% 
                 100.0% 
               
               
                 349_MARWOLEN1 
                 42.7% 
                 42.7% 
                 44.8% 
                 47.0% 
                 43.8% 
                 42.7% 
               
               
                 SB100X 
                 43.4% 
                 45.5% 
                 46.0% 
                 48.2% 
                 46.2% 
                 51.3% 
               
               
                 14_Tc1-8B_DR 
                 41.2% 
                 41.2% 
                 43.3% 
                 47.3% 
                 48.3% 
                 48.0% 
               
               
                 54_Tc1-4_Xt 
                 38.7% 
                 39.0% 
                 44.9% 
                 47.0% 
                 48.9% 
                 47.5% 
               
               
                 29_Tc1-3_FR 
                 39.0% 
                 40.2% 
                 42.2% 
                 50.0% 
                 50.2% 
                 47.2% 
               
               
                 25_Tc1-3_Xt 
                 39.6% 
                 41.3% 
                 43.1% 
                 50.0% 
                 51.1% 
                 47.5% 
               
               
                 43_Tc1-1_PM 
                 39.9% 
                 42.2% 
                 42.5% 
                 48.5% 
                 50.5% 
                 49.0% 
               
               
                 52_Tc1-16_Xt 
                 40.4% 
                 41.8% 
                 40.6% 
                 43.1% 
                 46.2% 
                 46.1% 
               
               
                 36_Tc1-1_Xt 
                 41.9% 
                 44.0% 
                 44.6% 
                 45.2% 
                 46.8% 
                 47.2% 
               
               
                 33_Tc1-10_Xt 
                 37.9% 
                 40.9% 
                 44.4% 
                 45.2% 
                 44.1% 
                 47.9% 
               
               
                 51_Tc1-11_Xt 
                 37.4% 
                 40.0% 
                 40.6% 
                 44.9% 
                 45.9% 
                 49.4% 
               
               
                 64_Mariner- 
                 24.6% 
                 24.3% 
                 17.9% 
                 20.4% 
                 22.2% 
                 19.8% 
               
               
                 5_XT 
               
               
                 70_Mariner- 
                 25.1% 
                 24.0% 
                 19.0% 
                 21.9% 
                 24.9% 
                 23.7% 
               
               
                 6_AMi 
               
               
                 142_Tigger4 
                 27.5% 
                 26.3% 
                 21.9% 
                 25.4% 
                 27.0% 
                 25.6% 
               
               
                 144_Tigger7 
                 28.7% 
                 28.7% 
                 24.2% 
                 25.4% 
                 25.2% 
                 26.7% 
               
               
                 45_Mariner- 
                 23.7% 
                 24.6% 
                 23.9% 
                 24.9% 
                 21.3% 
                 24.8% 
               
               
                 4_AMi 
               
               
                 90_Mariner- 
                 24.6% 
                 25.4% 
                 25.9% 
                 22.2% 
                 21.6% 
                 24.0% 
               
               
                 3_Crp 
               
               
                   
                 56_Tc1- 
                 59_TC1_FR2 
                 39_Tc1DR3_Xt 
                 35_Mariner2_AG 
                 37_Tc1- 
                 32_Tc1- 
               
               
                   
                 15_Xt 
                   
                   
                   
                 1_AG 
                 5_Xt 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 56_Tc1-15_Xt 
                   
                   
                   
                   
                   
               
               
                   
                 59_TC1_FR2 
               
               
                   
                 39_Tc1DR3_Xt 
               
               
                   
                 35_Mariner2_AG 
               
               
                   
                 37_Tc1-1_AG 
               
               
                   
                 32_Tc1-5_Xt 
               
               
                   
                 349_MARWOLEN1 
                 100.0% 
               
               
                   
                 SB100X 
                 47.2% 
                 100.0% 
               
               
                   
                 14_Tc1-8B_DR 
                 44.5% 
                 63.0% 
                 100.0% 
               
               
                   
                 54_Tc1-4_Xt 
                 46.9% 
                 63.0% 
                 69.8% 
                 100.0% 
               
               
                   
                 29_Tc1-3_FR 
                 45.7% 
                 63.6% 
                 71.6% 
                 76.8% 
                 100.0% 
               
               
                   
                 25_Tc1-3_Xt 
                 46.6% 
                 61.9% 
                 71.3% 
                 80.6% 
                 86.8% 
               
               
                   
                 43_Tc1-1_PM 
                 46.3% 
                 61.6% 
                 71.3% 
                 82.1% 
                 87.4% 
               
               
                   
                 52_Tc1-16_Xt 
                 44.8% 
                 59.8% 
                 60.5% 
                 53.1% 
                 56.0% 
               
               
                   
                 36_Tc1-1_Xt 
                 45.7% 
                 62.2% 
                 63.0% 
                 59.5% 
                 61.3% 
               
               
                   
                 33_Tc1-10_Xt 
                 42.7% 
                 60.6% 
                 63.8% 
                 61.2% 
                 62.9% 
               
               
                   
                 51_Tc1-11_Xt 
                 45.7% 
                 61.8% 
                 63.2% 
                 60.3% 
                 61.5% 
               
               
                   
                 64_Mariner- 
                 18.2% 
                 23.2% 
                 21.1% 
                 19.6% 
                 19.1% 
               
               
                   
                 5_XT 
               
               
                   
                 70_Mariner- 
                 20.9% 
                 26.7% 
                 24.6% 
                 24.0% 
                 24.0% 
               
               
                   
                 6_AMi 
               
               
                   
                 142_Tigger4 
                 23.6% 
                 26.4% 
                 25.4% 
                 24.6% 
                 26.4% 
               
               
                   
                 144_Tigger7 
                 23.3% 
                 28.7% 
                 24.9% 
                 27.6% 
                 25.8% 
               
               
                   
                 45_Mariner- 
                 23.3% 
                 29.3% 
                 25.1% 
                 27.9% 
                 24.9% 
               
               
                   
                 4_AMi 
               
               
                   
                 90_Mariner- 
                 24.8% 
                 27.3% 
                 23.7% 
                 24.9% 
                 22.3% 
               
               
                   
                 3_Crp 
               
               
                   
                   
                 349_MARWOLEN1 
                 SB100X 
                 14_Tc1- 
                 54_Tc1- 
                 29_Tc1- 
               
               
                   
                   
                   
                   
                 8B_DR 
                 4_Xt 
                 3_FR 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 25_Tc1-3_Xt 
                 100.0 
                   
                   
                   
                   
                   
                   
               
               
                 43_Tc1-1_PM 
                 88.0 
                 100.0 
               
               
                 52_Tc1-16_Xt 
                 56.6 
                 56.3 
                 100.0 
               
               
                 36_Tc1-1_Xt 
                 60.1 
                 61.0 
                 66.3 
                 100.0 
               
               
                 33_Tc1-10_Xt 
                 64.1 
                 63.5 
                 57.4 
                 64.7 
                 100.0 
               
               
                 51_Tc1-11_Xt 
                 61.8 
                 60.0 
                 60.3 
                 65.9 
                 70.9 
                 100.0 
               
               
                 64_Mariner- 
                 19.9 
                 19.9 
                 21.4 
                 22.9 
                 21.8 
                 19.4 
                 100.0 
               
               
                 5_XT 
               
               
                 70_Mariner- 
                 24.0 
                 24.0 
                 24.6 
                 25.8 
                 20.6 
                 21.2 
                 56.3 
               
               
                 6_AMi 
               
               
                 142_Tigger4 
                 24.9 
                 24.0 
                 24.9 
                 24.0 
                 22.1 
                 23.5 
                 29.1 
               
               
                 144_Tigger7 
                 27.0 
                 26.4 
                 24.9 
                 27.6 
                 23.5 
                 24.1 
                 28.6 
               
               
                 45_Mariner- 
                 25.5 
                 26.7 
                 26.7 
                 27.6 
                 24.1 
                 25.9 
                 27.0 
               
               
                 4_AMi 
               
               
                 90_Mariner- 
                 23.8 
                 23.2 
                 24.6 
                 23.5 
                 22.1 
                 24.4 
                 27.7 
               
               
                 3_Crp 
               
               
                 % 
                 25_Tc1- 
                 43_Tc1- 
                 52_Tc1- 
                 36_Tc1- 
                 33_Tc1- 
                 51_Tc1- 
                 64_Mariner- 
               
               
                   
                 3_Xt 
                 1_PM 
                 16_Xt 
                 1_Xt 
                 10_Xt 
                 11_Xt 
                 5_XT 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 25_Tc1-3_Xt 
                   
                   
                   
                   
                   
               
               
                   
                 43_Tc1-1_PM 
               
               
                   
                 52_Tc1-16_Xt 
               
               
                   
                 36_Tc1-1_Xt 
               
               
                   
                 33_Tc1-10_Xt 
               
               
                   
                 51_Tc1-11_Xt 
               
               
                   
                 64_Mariner- 
               
               
                   
                 5_XT 
               
               
                   
                 70_Mariner- 
                 100.0 
               
               
                   
                 6_AMi 
               
               
                   
                 142_Tigger4 
                 31.0 
                 100.0 
               
               
                   
                 144_Tigger7 
                 30.1 
                 41.3 
                 100.0 
               
               
                   
                 45_Mariner- 
                 29.2 
                 40.1 
                 50.5 
                 100.0 
               
               
                   
                 4_AMi 
               
               
                   
                 90_Mariner- 
                 27.8 
                 41.8 
                 46.7 
                 55.4 
                 100.0 
               
               
                   
                 3_Crp 
               
               
                   
                 % 
                 70_Mariner- 
                 142_Tigger4 
                 144_Tigger7 
                 45_Mariner- 
                 90_Mariner- 
               
               
                   
                   
                 6_AMi 
                   
                   
                 4_AMi 
                 3_Crp