Patent Publication Number: US-2003228670-A1

Title: Synthetic enzymes for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid and their use

Description:
[0001] The present invention relates to synthetic enzymes for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid, the use thereof for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid, DNA coding for the aforementioned enzymes and microorganisms transformed therewith.  
       [0002] The first article relating to the degradation of eugenol was written by Tadasa in 1977 (Degradation of eugenol by a microorganism. Agric. Biol. Chem. 41, 925-929). It describes the degradation of eugenol with a soil isolate which was presumed to be Corynebacterium sp. In this process ferulic acid and vanillin were identified as intermediate degradation products and the subsequent degradation was assumed to proceed via vanillic acid and protocatechuic acid.  
       [0003] In 1983 another article by Tadasa and Kyahara appeared (Initial Steps of Eugenol Degradation Pathway of a Microorganism. Agric. Biol. Chem. 47, 2639-2640) on the initial steps of eugenol degradation, this time with a soil isolate which was identified to be Pseudomonas sp. In this article eugenol oxide, coniferyl alcohol and coniferylaldehyde were described as intermediates for the formation of ferulic acid.  
       [0004] Also in 1983 a report by Sutherland et al. appeared (Metabolism of cinnamic, p-coumaric, and ferulic acids by  Streptomyces setonii.  Can. J. Microbiol. 29, 1253-1257) on the metabolism of cinnamic, p-coumaric and ferulic acids by  Streptomyces setonii.  In this process ferulic acid was degraded via vanillin, vanillic acid and protocatechuic acid, the ring-cleaving enzymes catechol 1,2-dioxygenase and protocatechuate 3,4-dioxygenase having been indirectly identified in the cell-free extract.  
       [0005] In 1985 Ötük (Degradation of Ferulic Acid by  Escherichia coli.  J. Ferment. Technol. 63, 501-506) reported on the degradation of ferulic acid by a strain of  Escherichia coli  isolated from decaying bark. Here as well vanillin, vanillic acid and protocatechuic acid were found as degradation products.  
       [0006] isoeugenol) for the microbial oxidation of eugenol and isoeugenol. However, only when isoeugenol was used the process did produce high conversion rates; the results were very poor using eugenol.  
       [0007] In 1991 EP-A 453 368 appeared [“Production de vanilline par bioconversion de précurseurs benzeniques” (Production of vanillin by the bioconversion of benzene precursors)], in which the reaction to form vanillin from vanillic acid and ferulic acid using a basidiomycete— Pycnoporus cinnabarinus  CNCM I-937 and 1-938—was observed.  
       [0008] In 1992 the Takasago Perfumery Company was granted a Japanese patent (Preparation of vanillin, coniferyl-alcohol and -aldehyde, ferulic acid and vanillyl alcohol—by culturing mutant belonging to Pseudomonas genus in presence of eugenol which is oxidatively decomposed; JP 05 227 980 21.1.1992) for the preparation of vanillin, coniferyl alcohol, coniferylaldehyde, ferulic acid and vanillyl alcohol from eugenol using a Pseudomonas mutant.  
       [0009] Also in 1992 U.S. Pat. No. 5,128,253 by Labuda et al. (Kraft General Foods) (Bioconversion process for the production of vanillin) was granted, in which a biotransformation process for the production of vanillin was described. Here as well the starting material was ferulic acid and the organisms used were  Aspergillus niger, Rhodotorula glutinis  and  Corynebacterium glutamicum.  The crucial feature was the use of sulphydryl components (e.g. dithiothreitol) in the medium. In 1993 the subject matter of the patent also appeared in the form of a publication (Microbial bioconversion process for the production of vanillin; Prog. Flavour Precursor Stud. Proc. Int. Conf. 1992, 477-482).  
       [0010] EP-A 542 348 (Process for the preparation of phenylaldehydes) describes a process for the preparation of phenylaldehydes in the presence of the enzyme lipoxygenase. Eugenol and isoeugenol are for example used as substrates. We have attempted to rework the process using eugenol, but have not succeeded in confirming the results of the reactions.  
       [0011] DE-A 4 227 076 [Verfahren zur Herstellung substitutierter Methoxyphenole und dafür geeigneter Mikroorganismus (Process for the production of substituted methoxyphenols and a microorganism suitable for said process)] describes the production of substituted methoxyphenols with a new Pseudomonas sp. The starting material used is eugenol and the products are ferulic acid, vanillic acid, coniferyl alcohol and coniferylaldehyde.  
       [0012] Also in 1995 a comprehensive review by Rosazza et al. (Biocatalytic transformation of ferulic acid: an abundant aromatic natural product; J. Ind. Microbiol. 15, 457-471) appeared on possible methods of biotransformation using ferulic acid.  
       [0013] The present invention relates to synthetic enzymes for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid from eugenol.  
       [0014] Synthetic enzymes according to the invention are for example:  
       [0015] a) eugenol hydroxylase,  
       [0016] b) coniferyl alcohol dehydrogenase,  
       [0017] c) coniferylaldehyde dehydrogenase,  
       [0018] d) ferulic acid deacylase and  
       [0019] e) vanillin dehydrogenase.  
       [0020] The invention also relates to DNA coding for the abovementioned enzymes and cosmid clones containing this DNA as well as vectors containing this DNA and microorganisms transformed with the DNA or the vectors. It also relates to the use of the DNA for the transformation of microorganisms for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid. The invention also relates to partial sequences of the DNA and functional equivalents. Functional equivalents are understood to be those derivatives in which individual nucleobases have been substituted (wobble substitutions) without resulting in any functional changes. In relation to proteins, amino acids can also be substituted without resulting in any functional changes.  
       [0021] The invention also relates to the individual steps for the production of coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin and vanillic acid from eugenol, i.e. in concrete terms:  
       [0022] a) the process for the production of coniferyl alcohol from eugenol carried out in the presence of eugenol hydroxylase;  
       [0023] b) the process for the production of coniferylaldehyde from coniferyl alcohol carried out in the presence of coniferyl alcohol dehydrogenase;  
       [0024] c) the process for the production of ferulic acid from coniferylaldehyde carried out in the presence of coniferylaldehyde dehydrogenase;  
       [0025] d) the process for the production of vanillin from ferulic acid carried out in the presence of ferulic acid deacylase;  
       [0026] e) the process for the production of vanillic acid from vanillin carried out in the presence of vanillin dehydrogenase.  
       [0027] After NMG mutagenesis mutants with defects in individual stages of the catabolism of eugenol were obtained from the eugenol-utilising Pseudomonas sp. strain HR 199 (DSM 7063). Using total DNA of wild-type Pseudomonas sp. HR 199 partially digested with EcoRI a gene library was constructed in the pVK100 cosmid, which has a broad host spectrum and can also be replicated in stable form in pseudomonads. After packaging in 1-phage particles the hybrid cosmids were transduced to  E. coli  S17-1. The gene library comprised 1330 recombinant  E. coli  S17-1 clones. The hybrid cosmid of each clone was transferred by conjugation into two eugenol-negative mutants (mutants 6164 and 6165) of the Pseudomonas sp. HR 199 strain and tested for a possible capacity for complementation. In this test two hybrid cosmids (pE207 and pE115) were identified, the obtainment of which restored mutant 6165&#39;s capacity to utilise eugenol. One hybrid cosmid (pE5-1) resulted in the complementation of mutant 6164.  
       [0028] The complementing capacity of plasmids pE207 and pE115 was attributed to a 23 kbp EcoRI fragment (E230). A physical map of this fragment was prepared and the fragment completely sequenced. The genes vanA and vanB which code for vanillate demethylase were localised in a 11.2 kbp HindIII subfragment (H110). Another open reading frame (ORF) was found to be homologous to g-glutamyl cysteine synthetase produced by  Escherichia coli.  An additional ORF, which was homologous to formaldehyde dehydrogenases, was identified between the aforementioned ORF and the vanB gene. Two additional ORF&#39;s were found to be homologous to the cytochrome C subunit or the flavoprotein subunit of p-cresol methylhydroxylase, respectively produced by  Pseudomonas putida.  In the Pseudomonas sp. HR 199 strain, these ORF&#39;s code for a new not previously described eugenol hydroxylase which converts eugenol into coniferyl alcohol via a quinone methide derivative by a process analogous to the reaction mechanism of p-cresol methyl hydroxylase. Another ORF of an unknown function was identified between the genes of the two subunits of eugenol hydroxylase. An ORF which was homologous to lignostilbene-a,b-dioxygenase was identified in a 5.0 kbp HindIII subfragment (H50). In addition one ORF was identified which was homologous to alcohol dehydrogenases. The structural gene vdh of vanillin dehydrogenase was identified in a 3.8 kbp HindIII/EcoRI subfragment. Upstream of this gene an ORF was localised which was homologous to enoyl-CoA hydratases produced by various organisms.  
       [0029] The complementing capacity of plasmid pE5-1 was attributed to the joint obtainment of the 1.2 and 1.8 kbp EcoRI fragments (E12 and E18). Fragment E 12 was completely, and fragment E 18 partially, sequenced. The structural gene cadh of coniferyl alcohol dehydrogenase, which contained an EcoRI cleavage site, was localised in these fragments. Using chromatographic methods the enzyme was isolated from the soluble fraction of the crude extract of cells of Pseudomonas sp. HR 199 grown on eugenol. An oligonucleotide sequence was deduced from the specific N-terminal amino acid sequence. A corresponding DNA probe hybridised with fragment E12, in which the region of the cadh gene encoding the N-terminus was localised.  
       [0030] A eugenol- and ferulic acid-negative mutant (mutant 6167) was complemented by obtaining a 9.4 kbp EcoRI fragment (E 94) of the hybrid cosmid pE5-1. A physical map of this fragment was prepared. The complementing property was localised in a 1.9 kbp EcoRI/HindIII subfragment. This fragment had incomplete ORF&#39;s (they extended beyond the EcoRI and HindIII cleavage sites) which were homologous to acetyl-CoA acetyl transferases of various organisms and to the “medium-chain acyl-CoA synthetase” produced by  Pseudomonas oleovorans.  Fragment E 94 was completely sequenced. Downstream of the aforementioned ORF&#39;s an ORF was located which was homologous to β-ketothiolases. The structural gene of coniferylaldehyde dehydrogenase (caldh) was localised in a central position of fragment E 94. Using chromatographic methods the enzyme was isolated from the soluble fraction of the crude extract of cells of Pseudomonas sp. HR 199 grown on eugenol.  
       [0031] The conjugative transfer of hybrid cosmid pE207 into a large number of Pseudomonas strains resulted in the heterologous expression of the van A, van B and vdh genes and the eugenol-hydroxylase genes in the transconjugants obtained. The obtainment of the plasmid of one strain allowed it to grow using eugenol as its carbon and energy source.  
       [0032] Materials and Methods  
       [0033] Growth conditions of the bacteria. Strains of  Escherichia coli  were grown at 37° C. in a Luria-Bertani (LB) or M9 mineral medium (Sambrook, J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.). Strains of Pseudomonas sp. and  Alcaligenes eutrophus  were grown at 30° C. in a nutrient broth (NB, 0.8% by weight) or in a mineral medium NM) (Schlegel, H. G. et al. 1961. Arch. Mikrobiol. 38: 209-222). Ferulic acid, vanillin, vanillic acid and protocatechuic acid were dissolved in dimethyl sulphoxide and added to the respective medium in a final concentration of 0.1% by weight. Eugenol was added to the medium directly in a final concentration of 0.1 vol.-%, or applied on filter paper (circular filters 595, Schleicher &amp; Schuell, Dassel, Germany) to the lids of MM agar plates. For the growth of transconjugants of Pseudomonas sp., tetracyline and kanamycin were used in final concentrations of 25 μg/ml and 300 μg/ml, respectively.  
       [0034] Nitrosoguanidine mutagenesis. The nitrosoguanidine mutagenesis of Pseudomonas sp. HR 199 was carried out using a modified method according to Miller (Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.). Instead of the citrate buffer, a potassium phosphate (PP) buffer (100 mM, pH 7.0) was used. The final concentration of N-methyl-N′-nitro-N-nitrosoguanidine was 200 μg/ml. The mutants obtained were screened with regard to the loss of their capacity to utilise eugenol, ferulic acid, vanillin and vanillic acid as growth substrates.  
       [0035] Qualitative and quantitative detection of metabolic intermediates in culture supernatants. Culture supernatants were analysed by high-pressure liquid chromatography (Knauer HPLC) either directly or after dilution with twice-distilled water. Chromatography was carried out on Nucleosil-100 C18 (7 μm, 250×4 mm). The solvent used was 0.1 vol.-% formic acid and acetonitrile.  
       [0036] Purification of coniferyl alcohol dehydrogenase and coniferylaldehyde dehydrogenase. The purification processes were carried out at 4° C.  
       [0037] Crude extract. Cells of Pseudomonas sp. HR 199 grown on eugenol were washed in a 10 mM sodium phosphate buffer with a pH of 7.5, resuspended in the same buffer and disrupted by being passed through a French press (Amicon, Silver Spring, Md., USA) twice at a pressure of 1,000 psi. The cell homogenate was subjected to ultracentrifugation (1 h, 100,000×g, 4° C.), the soluble fraction of the crude extract being obtained as the supernatant.  
       [0038] Anion exchange chromatography on DEAE Sephacel. The soluble fraction of the crude extract was dialysed overnight against a 10 mM sodium phosphate buffer with a pH of 7.5 containing 100 mM NaCl. The dialysate was applied to a DEAE Sephacel column (2.6 cm×35 cm, bed volumn [BV]: 186 ml) equilibrated with a 10 mM sodium phosphate buffer of a pH of 7.5 containing 100 mM NaCl at a flow rate of 0.8 ml/min. The column was washed with two bed volumes of a 10 mM sodium phosphate buffer with a pH of 7.5 containing 100 mM NaCl. The elution of coniferyl alcohol dehydrogenase (CADH) and coniferylaldehyde dehydrogenase (CALDH) was carried out with a linear salt gradient of 100 to 500 mM NaCl in a 10 mM sodium phosphate buffer with a pH of 7.5 (2×150 ml). 5 ml fractions were collected. Fractions with high CADH and CALDH activities were combined in the corresponding DEAE pools respectively.  
       [0039] Gel filtration chromatography on Sephadex G200. The CADH DEAE pool was concentrated in a 50 ml Amicon ultrafiltration chamber via a Diaflo ultrafiltration membrane PM 30 (both from AMICON CORP., Lexington, USA) at a pressure of 290 kPa to a volume corresponding to approx. 2% of the Sephadex G200-BV. The concentrated protein solution was applied to a Sephadex G200 column (BV: 138 ml) equilibrated with a 10 mM sodium phosphate buffer with a pH of 7.5 containing 100 mM NaCl and eluted with the same buffer at a flow rate of 0.2 ml/min. 2 ml fractions were collected. Fractions with a high CADH activity were combined in the Sephadex G200 pool.  
       [0040] Hydrophobic interaction chromatography on butyl Sepharose 4B. The CADH Sephadex G200 pool was adjusted to 3 M NaCl and then applied to a butyl Sepharose 4B column (BV: 48 ml) equilibrated with a 10 mM sodium phosphate buffer with a pH of 7.5 containing 3 M NaCl (flow rate: 0.5 ml/min). The column was then washed with 2 BV of a 10 mM sodium phosphate buffer with a pH of 7.5 containing 3 M NaCl (flow rate: 1.0 ml/min). CADH was eluted with a linearly decreasing NaCl gradient of 3 to 0 M NaCl in a 10 mM sodium phosphate buffer with a pH of 7.5 (2×50 ml). 4 ml fractions were collected. Fractions with a high CADH activity were combined in the HIC pool and concentrated as described above.  
       [0041] Chromatography on hydroxyapatite. The CALDH DEAE pool was concentrated to 10 ml in a 50 ml Amicon ultrafiltration chamber via a Diaflo ultrafiltration membrane PM 30 (both from AMICON CORP., Lexington, USA) at a pressure of 290 kPa. The concentrated protein solution was applied to a hydroxyapatite column (BV: 80 ml) equilibrated with a buffer (10 mM NaCL in a 10 mM sodium phosphate buffer with a pH of 7.0) (flow rate: 2 ml/min). The column was then washed with 2.5 bed volumes of a buffer (flow rate: 2 ml/min). CALDH was eluted with a linearly increasing sodium phosphate gradient of 10 to 400 mM NaP (in each case containing 10 mM NaCL) (2×100 ml). 10 ml fractions were collected. Fractions with high CALDH activity were combined in the CALDH HA pool.  
       [0042] Gel filtration chromatography on Superdex HR 200 10/30. The CALDH HA pool was concentrated to 200 μl (Amicon ultrafiltration chamber, ultrafiltration membrane PM 30) and applied to a Superdex HR 200 10/30 column (13V: 23.6 ml) equilibrated with a 10 mM sodium phosphate buffer with a pH of 7.0. CALDH was eluted with the same buffer at a flow rate of 0.5 ml/min. 250 μl fractions were collected. Fractions with high CALDH activity were combined in the CALDH Superdex pool.  
       [0043] Determination of coniferyl alcohol dehydrogenase activity. The CADH activity was determined at 30° C. by means of an optical enzymatic test according to Jaeger et al. (Jaeger, E., L. Eggeling and H. Sahm. 1982. Current Microbiology. 6: 333-336) with the aid of a ZEISS PM 4 spectrophotometer fitted with a TE converter (both from ZEISS, Oberkochen, Germany) and a recorder. The reaction mixture with a volume of 1 ml contained 0.2 mmol of Tris/HCl (pH 9.0), 0.4 μmol of coniferyl alcohol, 2 μmol of NAD, 0.1 mmol of semicarbazide and a solution of the enzyme (“Tris”=tris(hydroxymethyl)-aminomethane). The reduction of NAD was monitored at 1=340 nm (e=6,3 cm 2 /μmol). The enzyme activity was recorded in units (U), 1 U corresponding to that quantity of enzyme which metabolises 1 μmol of substrate per minute. The protein concentrations in the samples were determined according to the method described -by Lowry et al. (Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. J. Biol. Chem. 193: 265-275).  
       [0044] Determination of the coniferylaldehyde dehydrogenase activity. The CALDH activity was determined at 30° C. by an optical enzymatic test with the aid of a ZEISS PM 4 spectrophotometer fitted with a TE converter (both from ZEISS, Oberkochen, Germany) and a recorder. The reaction mixture of a volume of 1 ml contained a 10 mM Tris/HCl buffer (pH 8.8), 5.6 mM coniferylaldehyde, 3 mM NAD and a solution of the enzyme. The oxidation of coniferylaldehyde to form ferulic acid was monitored at 1=400 nm (e=34 cm 2 /μmol). The enzyme activity was recorded in units (U), 1 U corresponding to that quantity of enzyme which metabolises 1 μmol of substrate per minute. The protein concentration in the samples was determined according to the method described by Lowry et al. (Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. J. Biol. Chem. 193: 265-275).  
       [0045] Electrophoretic methods. The separation of protein-containing extracts was carried out in 7.4% by weight polyacrylamide gels under native conditions according to the method described by Stegemann et al. (Stegemann et al. 1973. Z. Naturforsch. 28c: 722-732) and under denaturing conditions in 11.5% by weight polyacrylamide gels according to the method described by Laemmli (Laemmli, U.K. 1970. Nature (London) 227: 680-685). Serva Blue R was used for non-specific protein staining. For specifically staining coniferyl alcohol, coniferylaldehyde and vanillin dehydrogenase the gels were placed for 20 mins in a new 100 mM PP buffer (pH 7.0) and then incubated at 30° C. in the same buffer, to which 0.08% by weight of NAD, 0.04% by weight of p-nitroblue-tetrazolium chloride, 0.003% by weight of phenazine methosulphate and 1 mM of the respective substrate had been added, until the corresponding coloured bands appeared.  
       [0046] The transfer of proteins from polyacrylamide gels to PVDF membranes. Proteins were transferred from SDS polyacrylamide gels to PVDF membranes (Waters-Milipore, Bedford, Mass., USA) with the aid of a semidry fast blot device (B32/33 from Biometra, Göttingen, Germany) according to the manufacturer&#39;s instructions.  
       [0047] Determination of N-terminal amino acid sequences. The determination of N-terminal amino acid sequences was carried out with the aid of a protein peptide sequencer (of type 477 A, Applied Biosystems, Foster City, USA) and a PTH analyser, according to the manufacturer&#39;s instructions.  
       [0048] Isolation and manipulation of DNA. The isolation of genomic DNA was carried out by the method described by Marmur (Marmur, J. 1961. Mol. Biol. 3: 208-218). Megaplasmid DNA was isolated according to the method described by Nies et al. (Nies, D., et al. 1987. J. Bacteriol. 169: 4865-4848). The isolation and analysis of other plasmid DNA or DNA restriction fragments, the packaging of hybrid cosmids in 1-phage particles and the transduction of  E. coli.  was carried out by standard methods (Sambrook, J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.).  
       [0049] Transfer of DNA. The preparation and transformation of competent  Escherichia coli  cells was carried out by the method described by Hanahan (Hanahan, D. 1983. J. Mol. Biol. 166: 557-580). Conjugative plasmid transfer between plasmid-containing  Escherichia coli  S17-1 strains (donor) and Pseudomonas sp. strains (recipient) and  Alcaligenes eutrophus  (recipient) was carried out on NB agar plates according to the method described by Friedrich et al. (Friedrich, B. et al. 1981. J. Bacteriol. 147: 198-205) or by a “minicomplementation method” on MM agar plates using 0.5% by weight of gluconate as the carbon source and 25 μg/ml of tetracylin or 300 μg/ml of kanamycin. In this process cells of the recipient were applied in one direction in the form of an inoculation line. After 5 minutes cells of the donor strains were then applied in the form of inoculation lines crossing the recipient inoculation line. After incubation for 48 h at 30° C. the transconjugants grew directly downstream of the crossing point, whereas neither the donor nor the recipient strain was capable of growth.  
       [0050] Hybridisation experiments. DNA restriction fragments were electrophoretically separated in an 0.8% by weight agarose gel in a 50 mM Tris, 50 mM boric acid and 1.25 mM EDTA buffer (pH 8.5) (Sambrook, J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.). The transfer of the denatured DNA from the gel to a positively charged nylon membrane (pore size: 0.45 μm, Pall Filtrationstechnik, Dreieich, Germany), the subsequent hybridisation with biotinylated or  32 P-labelled DNA probes and the production of these DNA probes was carried out according to standard methods (Sambrook, J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.).  
       [0051] The synthesis of oligonucleotides. Using desoxynucleoside phosphoramidites as the starting material, oligonucleotides were synthesised on a 0.2 μmol scale (Beaucage, S. L., and M. H. Caruthers. 1981. Tetrahedron Lett. 22: 1859-1862). The synthesis was carried out in a Gene Assembler Plus according to the manufacturer&#39;s instructions (Pharmacia-LKB, Uppsala, Sweden). The elimination of the protecting groups was carried out by incubation for 15 h at 55° C. in a 25 vol.-% aqueous ammonia solution. The oligonucleotides were finally purified in an NAP-5 column (Pharmacia-LKB, Uppsala, Sweden).  
       [0052] DNA sequencing. The determination of nucleotide sequences was carried out by the didesoxy chain termination method described by Sanger et al. (Sanger et al. 1977. Proc. Natl. Acad. Sci. USA 74: 5463-5467) using [α- 35 S]dATP and a T7 polymerase sequencing kit (Pharmacia-LKB). 7-Deazaguanosine-5′-triphosphate was used instead of dGTP (Mizusawa, S. et al. 1986. Nucleic Acids Res.14: 1319-1324). The products of the sequencing reactions were separated in a 6% by weight polyacrylamide gel in a 100 mM Tris/HCl, 83 mM boric acid and 1 mM EDTA buffer (pH 8.3) containing 42% by weight urea, an S2 sequencing apparatus (GIBCO/BRL, Bethesda Research Laboratories GmbH, Eggenstein, Germany) being used according to the manufacturer&#39;s instructions. After electrophoresis the gels were incubated for 30 mins in 10 vol.-% acetic acid and, after washing briefly in water, dried for 2 hours at 80° C. Kodak X-OMAT AR X-ray films (Eastman Kodak Company, Rochester, N.Y., USA) were used for the autoradiography of the dried gels. In addition DNA sequences were also determined “non-radioactively” with the aid of an “LI-COR DNA Sequencer Model 4000L” (LI-COR Inc., Biotechnology Division, Lincoln, Nebr., USA) using a “Thermo Sequenase fluorescent labelled primer cycle sequencing kit with 7-deaza-dGTP” (Amersham Life Science, Amersham International plc, Little Chalfont, Buckinghamshire, England), in each case according to the manufacturer&#39;s instructions.  
       [0053] Various sequencing strategies were used: With the aid of synthetic oligonucleotides sequencing was carried out by the “Primer-hopping Strategy” described by Strauss et al. (Strauss, E. C. et al. 1986. Anal. Biochem. 154: 353-360). If only “universal” and “reverse primers” were used hybrid plasmids were used as “template DNA”, the inserted DNA fragments of which had been unidirectionally shortened with the aid of an “Exo III/Mung Bean Nuclease Deletion” kit (Stratagene Cloning Systems, La Jolla, Calif., USA) according to the manufacturer&#39;s instructions.  
       [0054] Chemicals, biochemicals and enzymes: Restriction enzymes, T4 DNA ligase, lambda DNA and enzymes and substrates for the optical enzymatic tests were obtained from C. F. Boehringer &amp; Söhne (Mannheim, Germany) or from GIBCO/BRL (Eggenstein, Germany). [a- 35 S]dATP and [g- 32 P]ATP were obtained from Amersham/Buchler (Braunschweig, Germany). NA-type agarose was obtained from Pharmacia-LKB (Uppsala, Sweden). All the other chemicals were from Haarmann &amp; Reimer (Holzminden, Germany), E. Merck AG (Darmstadt, Germany), Fluka Chemic (Buchs, Switzerland), Serva Feinbiochemica (Heidelberg, Germany) or Sigma Chemie (Deisenhofen, Germany).  
     
    
    
     EXAMPLES  
     Example 1  
     [0055] The Isolation of Mutants of the Pseudomonas sp. HR 199 Strain with Defects in the Catabolism of Eugenol  
     [0056] The Pseudomonas sp. HR 199 strain was subjected to nitrosoguanidine mutagenesis in order to isolate mutants with defects in the catabolism of eugenol. The mutants obtained were classified according to their capacity to utilise eugenol, ferulic acid and vanillin as their carbon and energy source. Mutants 6164 and 6165 were no longer capable of utilising eugenol as a carbon and energy source, although, as in the case of the wild type, they were capable of utilising ferulic acid and vanillin. Mutants 6167 and 6202 were no longer capable of utilising eugenol and ferulic acid as their carbon and energy source, although, as in the case of the wild type, they were capable of utilising vanillin. The abovementioned mutants were used in the subsequent molecular-biological analyses.  
     Example 2  
     [0057] Construction of a Pseudomonas sp. HR 199 Gene Library in the Cosmid Vector  
     [0058] The genomic DNA of the Pseudomonas sp. HR 199 strain was isolated and subjected to partial restriction digestion with EcoRI. The DNA preparation thus obtained was ligated with vector pVK100 cut by EcoRI. The DNA concentrations were relatively high in order to accelerate the formation of concatemeric ligation products. The ligation materials were packaged in 1-phage particles which were subsequently used for transduction of  E. coli  S17-1. The selection of the transductants was carried out on tetracycline-containing LB agar plates. In this manner 1330 transductants were obtained which contained various hybrid cosmids.  
     Example 3  
     [0059] The Identification of Hybrid Cosmids Containing Essential Genes of Eugenol Catabolism  
     [0060] The hybrid cosmids of the 1330 transductants were transferred conjugatively to mutants 6164 and 6165 by a minicomplementation process. The resulting transconjugants were examined on MM plates containing eugenol for their capacity to grow again on eugenol (complementation of the respective mutant). Mutant 6164 was complemented by the obtainment of hybrid cosmid pE5-1, which contained a 1.2 kbp, a 1.8 kbp, a 3 kbp, a 5.8 kbp and a 9.4 kbp EcoRI fragment in cloned form. The  E. coli  S17-1 strain containing this hybrid cosmid was deposited at the “Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH” (DSM) under the number DSM 10440. Mutant 6165 was complemented by the obtainment of the hybrid cosmids pE207 or pE115 respectively. The complementing capacity was attributed to a 23 kbp EcoRI fragment which was contained in cloned form in the hybrid cosmid pE207 as the only EcoRI fragment, whereas hybrid cosmid pE115 additionally contained a 3 kbp and a 6 kbp EcoRI fragment. The  E. coli  S17-1 strain containing hybrid cosmid pE207 was deposited at the DSM under the number DSM 10439.  
     Example 4  
     [0061] The Analysis of the 23 kbp EcoRI Fragment (E230) of the Hybrid Cosmid pE207  
     [0062] Fragment E230 was isolated preparatively from EcoRI-digested hybrid cosmid pE207 and ligated to pBluescript SK − -DNA digested with EcoRI. Using the ligation material  E. coli  XL1-Blue was transformed. Following “blue-white” selection on LB-Tc-Amp agar plates containing X-Gal and IPTG, “white” transformants were obtained whose hybrid plasmids pSKE230 contained the fragment E230 in cloned form. With the aid of this plasmid and by using various restriction enzymes a physical map of the fragment E230 was prepared (FIG. 1).  
     [0063] By cloning subfragments of E230 in vectors pVK101 and pMP92, both of which have a broad host specturm and are also stable in pseudomonads, followed by conjugative transfer into mutant 6165, the region complementing mutant 6165 was localised in a 1.8 kbp KpnI fragment (K18). After cloning this fragment in pBluescript SK −  the nucleotide sequence was determined, the gene of the cytochrome C subunit of eugenol hydroxylase being identified. The gene product of 117 amino acids had an N-terminal leader peptide (MMNVNYKAVGAS-LLLAFISQGAWA) and 32.9% identity (via a region of 82 amino acids) with the cytochrome C subunit of p-cresol methylhydroxylase produced by  Pseudomonas putida  (McIntire et al. 1986. Biochemistry 25:5975-5981).  
     [0064] By cloning the KpnI subfragments of E230 adjacent to K18 in pBluescript SK −  and sequencing, additional open reading frames (ORF) were identified, one of which codes for the flavoprotein subunit of eugenol hydroxylase and was highly homologous to the flavoprotein subunit of p-cresol methylhydroxylase produced by  Pseudomonas putida.  An additional ORF was found to be highly homologous to g-glutamyl cysteine synthetase (the first enzyme in the biosynthesis of glutathione) produced by  Escherichia coli  (Watanabe et al. 1986. Nucleic Acids Res. 14: 4393-4400).  
     [0065] In the soluble fraction of the crude extract of  E. coli  (pSKE230) vanillin dehydrogenase was detected by specific activity staining in a polyacrylamide gel. By subcloning in pBluescript SK −  and analysis of soluble fractions of the crude extracts of the transformants obtained, the vanillin dehydrogenase gene (vdh) was localised in a 3.8 kbp HindIII/EcoRI subfragment of E230. The complete nucleotide sequence of this fragment was determined. The molecular weight of the vanillin dehydrogenase was 50,779, as confirmed by SDS polyacrylamide gel electrophoresis. The amino acid sequence was highly homologous to other aldehyde dehydrogenases of various origins.  
     [0066] Upstream of the vdh gene an additional ORF was identified which was homologous to enoyl-CoA hydratases. The calculated molecular weight of 27,297 was confirmed by SDS polyacrylamide gel electrophoresis.  
     [0067] By sequencing the 5.0 kbp HindIII subfragment of E230, which had also been cloned in pBluescript SK − , an ORF was identified which was highly homologous to the lignostilbene-a,b-dioxygenase produced by  Pseudomonas paucimobilis.  By complete sequencing of the fragment E230 two additional ORF&#39;s were identified which were homologous to formaldehyde-dehydrogenases (fdh) and alcohol dehydrogenases (adh) (cf. FIG. 1).  
     Example 5  
     [0068] The Analysis of the Region of Hybrid Cosmid pE5-1 Complementing Mutant 6164  
     [0069] Mutant 6164 was complemented by the obtainment of hybrid cosmid pE5-1 which contained a 1.2 kbp (E12), a 1.8 kbp (E18), a 3 kbp (E30), a 5.8 kbp (E58) and a 9.4 kbp (E94) EcoRI fragment in cloned form (FIG. 1). By digesting pE5-1 with EcoRI and subsequent religation a derivative (pE106) of this hybrid cosmid was obtained which only contained fragments E12, E18 and E30. Following conjugative transfer into mutant 6164 this plasmid was however capable of complementing the latter, as a result of which corresponding transconjugants were once again capable of growing on eugenol as a carbon and energy source.  
     [0070] After digesting plasmid pE106 with EcoRI, gel-electrophoretic separation of the digestion material in a 0.8% by weight agarose gel and transfer of the DNA to a nylon membrane, hybridisation was carried out with a  32 P-labelled oligonucleotide probe of the following sequence:  
                          ATG CAA CTC ACC AAC AAA AAA ATC GT-3′                G   G   C   T   G   G   T           G   G   C       G   G           G   T   G       G   G                   G       G   G                   T       G   G          
 
     [0071] The sequence of this gene probe had been deduced from the N-terminal amino acid sequence of coniferyl alcohol dehydrogenase (CADH) (see below) purified from Pseudomonas sp. HR 199. With the aid of this probe the region of the cadh gene encoding the N-terminus of the CADH was localised in fragment E12. This fragment and parts of the adjacent fragment E 18 were also sequenced and the complete sequence of the cadh gene thus determined. The amino acid sequence deduced from cadh was homologous to other alcohol dehydrogenases of class I, group II (according to Matthew and Fewson. 1994. Critical Rev. Microbiol. 20(1): 13-56).  
     Example 6  
     [0072] Purification and Characterisation of Coniferyl Alcohol Dehydrogenase  
     [0073] Pseudomonas sp. HR 199 was grown on eugenol. The cells were harvested, washed and disrupted with the aid of a French press. The soluble fraction of the crude extract obtained after ultracentrifugation had a specific activity of 0.24 U/mg of protein. By means of chromatography on DEAE Sephacel an 11.7-fold enrichment of CADH was obtained in a yield of 83.7%. By means of chromatography on Sephadex G200 a 6.8-fold enrichment of CADH was obtained in a yield of 11.2%. By means of chromatography on butyl Sepharose 4B a 70.6-fold enrichment of CADH was obtained in a yield of 7.8%.  
     [0074] With the aid of this method a preparation was obtained which displayed a band at 27 kDa according to SDS polyacrylamide gel electrophoresis. The purification factor was 64 and the yield 0.8%.  
     [0075] Optimum Temperature and Thermal Stability  
     [0076] The optimum temperature for the reaction catalysed with CADH was 42° C. The enzyme was however sensitive to heat. The half-lives were as follows: T 1/2  (34° C.)=5 mins, T 1/2  (39° C.)=1 min, T 1/2  (42° C.)&lt;1 min.  
     [0077] Optimum pH  
     [0078] The optimum pH for the reaction catalysed by CADH was 10.9 in a 25 mM MOPS buffer. At higher pH values a decrease in activity due to denaturation was observed.  
     [0079] Apparent Molecular Weight  
     [0080] The molecular weight of native CADH was determined with the aid of FPLC by gel filtration on Superdex 200HR 10/30 at 54.9 kDa, which suggests a a 2  subunit structure.  
     [0081] N-Terminal Amino Acid Sequence  
     [0082] The determination of the N-terminal amino acid sequence of the purified protein revealed the following result:  
                          1       5         10            15               20           M Q L T N K K I V V V (G) V (S) ? (R) (I) ? (A) (E)                             (V)   (V)          
 
     [0083] (Sequence in the single letter code; ?: definition not possible; ( ): not certain; in the second row an amino acid is mentioned which may also apply)  
     Example 7  
     [0084] Purification and Characterisation of Coniferylaldehyde Dehydrogenase  
     [0085] Pseudomonas sp. HR 199 was grown on eugenol. The cells were harvested, washed and disrupted with the aid of a French press. The soluble fraction of the crude extract obtained after ultracentrifugation displayed a specific activity of 0.43 U/mg protein. By chromatography on DEAE Sephacel a 6.6-fold enrichment of CALDH was obtained in a yield of 65.3%. By chromatography on hydroxy-apatite a 63-fold enrichment of CALDH was obtained in a yield of 33%. By chromatography on Superdex HR 200 an 81-fold enrichment of CALDH was obtained in a yield of 13%. With the aid of this method a preparation was obtained which, according to SDS polyacryamide gel electrophoresis, displayed a band at approx. 49 kDa.  
     [0086] Optimum Temperature and Thermal Stability  
     [0087] The optimum temperature of the reaction catalysed by CALDH was 26° C. The enzyme was sensitive to heat. The half-lives were as follows: T 1/2  (31° C.)=5 mins, T 1/2  (34° C.)=2.5 mins, T 1/2  (38° C.)=1 min.  
     [0088] Optimum pH  
     [0089] The optimum pH for the reaction catalysed by CALDH was 8.8 in a 100 mM Tris/HCl buffer. At this pH value the enzyme is however already unstable (87 % decrease in activity within 5 mins). At lower pH values the enzyme is more stable (e.g. pH 6.0: 50% decrease in activity within 4 hours).  
     [0090] Substrate Specificity  
     [0091] The enzyme not only accepts coniferylaldehyde (100%) but also trans-cinnamaldehyde (96.7%), sinapyl aldehyde (76.7%), p-anisaldehyde (23.1%), benzaldehyde (17.8%), 3,5-dimethoxy-benzaldehyde (7.6%) and 3-hydroxy-benzaldehyde (1.7%) as substrates.  
     [0092] The K M  value of CALDH for coniferylaldehyde is in the range between 0.007 and 0.012 mM at a V max  of approx. 9 to 15 U/ml. The K M  value of CALDH for NAD is 0.334 mM at a V max  of 14.2 U/ml. Compared with NAD, NADP is accepted at a rate of 4.3%.  
     [0093] N-Terminal Amino Acid Sequence  
     [0094] The determination of the N-terminal amino acid sequence of the purified protein revealed the following result:  
                          1 S I L G L N G A P V G A E Q L G S A L (D) 20              
 
     [0095] (seqence in the one-letter code; ( ): not certain).  
     Example 8  
     [0096] Localisation and Sequencing of the Coniferylaldehyde Dehydrogenase Gene (caldh)  
     [0097] The N-terminal amino acid sequence was definitively assigned to an amino acid sequence deduced from the DNA sequence of fragment E94 of plasmid pE5-1. Thus the CALDH structural gene caldh is localised in E94. The amino acid sequence deduced from caldh was homologous to other aldehyde dehydrogenases.  
     Example 9  
     [0098] The Complementation of other Mutants Displaying Defects in the Catabolism of Eugenol using Hybrid Cosmids pE207 and pE5-1  
     [0099] Following NMG mutagenesis, mutants 6167 and 6202 had been obtained which were no longer capable of utilising eugenol and ferulic acid as their carbon and energy source (see above). The obtainment of plasmid pE207 meant that, after conjugative transfer, mutant 6202 was once again capable of utilising the aforementioned substrates. This mutant is complemented by the gene homologous to enoyl-CoA hydratase.  
     [0100] The obtainment of plasmid pE5-1 meant that, after conjugative transfer, mutant 6167 was once again capable of utilising the abovementioned substrates. By individually cloning the EcoRI fragments of pE5-1 in pHP 1014 and the conjugative transfer of these plasmids into mutant 6167 the complementing property was localised in fragment E94. A physical map of fragment E94 was prepared after cloning in pBluescript SK −  and digestion with various restriction enzymes. By cloning subfragments of E94 in the vectors pVK101 and pMP92, followed by conjugative transfer into mutant 6167, the region complementing mutant 6167 was localised in a 1.9 kbp EcoRI/HindIII fragment (EH19). After cloning this fragment in pBluescript SK −  and sequencing, 2 ORF&#39;s were identified which were homologous to acetyl-CoA acetyltransferases and to “medium-chain acyl-CoA synthetase” produced by  Pseudomonas oleovorans.  By completely sequencing fragment E94, additional ORF&#39;s were identified which were homo-logous to regulator proteins and a chemotaxis protein (cf. FIG. 1).  
     Example 10  
     [0101] Determination of the Chromosomal Coding of the Genes for the Catabolism of Eugenol in Pseudomonas sp. HR 199  
     [0102] Since Pseudomonas sp. HR 199 has a megaplasmid of a size of approx. 350 kbp, a hybridisation experiment was carried out to examine whether the genes for the catabolism of eugenol were localised in this megaplasmid or in the chromosome. For this purpose megaplasmid preparations of the wild type and of the mutants were separated in an 0.8% by weight agarose gel. The chromosomal and megaplasmid DNA was blotted onto a nylon membrane and then hybridised against a biotinylated HE38 DNA probe. A hybridisation signal was only obtained with the chromosomal DNA and not with the megaplasmid DNA. Thus the genes for the catabolism of eugenol in Pseudomonas sp. HR 199 are coded in the chromosome.  
     Example 11  
     [0103] The Heterologous Expression of Genes for the Catabolism of Eugenol from Pseudomonas sp. HR 199 in other Pseudomonas Strains and in  Alcaligenes eutrophus.    
     [0104] The plasmid pE207 and a pVK101 hybrid plasmid containing fragment H110 (pVKH110) were conjugatively transferred to  A. eutrophus  and into Pseudomonas strains which were not capable of metabolising eugenol, vanillin or vanillic acid. The transconjugants obtained were not only examined for their capacity to grow on MM agar plates containing eugenol, vanillin or vanillic acid but also some transconjugants were incubated with eugenol in an MM liquid medium. By means of HPLC analysis of the culture supernatants some of the transconjugants were found to metabolise eugenol.  
     [0105] In this analysis the functional expression of the vdh gene in transconjugants of  P. stutzeri, P. asplenii,  Pseudomonas sp. DSM13, Pseudomonas sp. DSM15a and Pseudomonas sp. D1 was determined.  
     [0106] Transconjugants of the strain Pseudomonas sp. D1, which contained the plasmid pE207, were capable of growing using eugenol as their carbon and energy source. In corresponding transconjugants of  P. testosteroni  LMD3324,  P. fluorescens  TypeB,  P. stutzeri  DSM 50027, Pseudomonas sp. DSM 1455 and  P. fragi DSM 3456 functional expression of the eugenol hydroxylase genes was also observed which resulted in the secretion of intermediates of the catabolism of eugenol (coniferyl alcohol, coniferylaldehyde, ferulic acid, vanillin, vanillic acid) into the culture medium. Growth of these transconjugants on eugenol was however not observed.  
    
     
       
         1 
         
           
             45  
           
           
             1  
             32679  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (3146)..(3997)  
               gene = “ORF1” 
             
           
            1 

gaattcatcc tcatggagca cttctacaag cagcaggcag gccaccctcc ccagaccgat     60 

gacgtgcata ttatcgcgat cggcggaacg agctttaaac gctacctgga gctcggaaag    120 

ctcctgaaca tcagagttgc cgcaattcga gataacgacg gtgactatca gcagaactgt    180 

gtagcgaact acgaaggcta cctgtacgag tcggccaaga ttttcgccgc cccagatcct    240 

gaccgaagca ccttcgaaat agggctgtac cgtgacaacc agaaagcctg tgacgatctc    300 

tttgttgcgg gtcgcaaaaa actgaccgtg caagagtaca tgctcaaaaa taaagcggat    360 

gccgctttcg agctgctgac caagaagtcc gctgaactga tcgccccgaa gtacatacag    420 

gaagcgatcg aatggataag agcgtaattt tctccgtcgc aggatccggg aaaaccagcc    480 

tgatcatcga gcgtctcagc cttgatcagc gggcattggt catcacttac acggacaaca    540 

atcaccggca cctgcgcaac aggatcattc agagattcgg ggtgatccca tccaacatca    600 

cgctcatgac gtacttctcg ttcctgcatg ggttctgcta tcggcccttg atgcaattgc    660 

agctaggaac acgaggccta aatttcagac gtccgcccaa caggcagtac cccctgaacg    720 

atctcaatcg gtatcgcgat ggaagcggca ggctctatca ctgccgcctc gcgaaactgc    780 

tggacgttgc gcaggcctta ccggatgtgc gtgcccgcct ggagcgcttt tacgactgcc    840 

tgtacgtcga cgaggtacag gatttcgcgg gtcacgactt caacctcctg ctggaggttt    900 

cacgggcgaa gatcggcatg acgttcgtcg gtgatttcca ccagcacacc ttcgatacca    960 

gccgagacgg agcggtaaac aaaacccttc acgacgatgc cgttcgctac gagaagcgct   1020 

ttcgtgatgc cggcatttcg gtggacaagc aaacgttgaa ccgcagctgg cgatgcgcca   1080 

aaacggtctg tgacttcatc agcgcaaagc tgaaaattgg cgatggacgc tcacgaggag   1140 

cggggcagcc ggatcattag agttgatgac caagagcagg ccaacttgtt gcacgttgac   1200 

ccaaccatcg tgaagctgtt tttgagcgaa cactacaagt acggctgcca ctccgaaaac   1260 

tggggggcaa gcaaggcatg gatcacttta acgatgtctg cgttgtgatg ggcccgggta   1320 

tctggaaaga ctatgtggct gagaggttac accaggccaa cccgcaaacc cgaaacaagc   1380 

tgtacgtggc ctgcactagg gcgcggggtg atctgtattt cgtgcctgag aagctcttga   1440 

gggccttcaa acagggaaat taggcgataa agctgaaaaa ggattttcaa gtaaagacca   1500 

ctccttcctt actcgatgtc cgcttttggc cgatttctgc cagtcacgac cggcaaagaa   1560 

cggccaaaag cggactgatg cggttactaa gcctgcctct tattgaagct tggtgggctt   1620 

taagaatgtg gtgcgatcca gcctgatgat gttccgcttt atgcacgcag ccaagcctat   1680 

cgaccgccgt ctgcacgttg taaccgacta cgcctgtgcc tttgccgctg gtggccatgg   1740 

agcgtgcatc cggatcggtg agtgagactt gcccatccgg tgcttcacgt agctgctgct   1800 

ccatctcctt gagcgcctgc atctgctggc ggagtttctc gattttatcc tggaggcggc   1860 

tggctttggc ttcggcgaca tcggattgag ttctgtcggc ggtgtccatc gctgccagat   1920 

agcggtcgat gattttatca atctggtcca tccgggcgcg cacccgctat gatccggagt   1980 

cctccgatat cgatgaggcc tatctgggct ggaagagcgg ttcggtgttc tcagaccttg   2040 

gcgagaacgc ggtcaagctc agcttcgggc gccaagcctt caagatcggc aacggcttcc   2100 

tgatcggcga aggccacgtc gaccaaggta acgatgcggg ctactggctg gcccctacct   2160 

aggcgttcga caacaccgtc ctagcccaac tggacaccgg caagctgcat gtcgacctgt   2220 

tcgacctcca ggcgggcatg gatctggacg tcgccgacat caaggagaaa gtccgggtgc   2280 

gcgggggcaa cgtcgagtgg cgcgacgaga cctacggcac ggtagggttc accggcttcc   2340 

atacgctgga cgctgacaat ccgctgcgcg acggcatgaa tgtctacgac gtacgcgcat   2400 

cgggcagccc gatccgagcc ctgccgcagg tggccctggc ggcggagtac gcctggcagc   2460 

gcggcggcga ggcggacaag acgagtgagg cctggtacct acagggcagc tacacctttc   2520 

gggatgcccc ctggacgcca gtgctgatgt accgtcacgc ggtcttctcc gacgactacg   2580 

actccctgct gtacggctaa gggggcaaca acatgggctg gaaaggagca ttgcgttgaa   2640 

acgatgctga agggcgtcac tcttttactg ctgtccgctc acgtcgaaac tgcatgattt   2700 

cgggcagcct ttcttctatc cagtcggcca gcacctgaac atgagccgct acttcctggc   2760 

caagcggcgt caggctgtac tcgacatgtg ggggaacgac cgggagcgaa tgtcgagcta   2820 

tgaaaccgtc tccctccagg ccttgtaggg tctgcgcaag cattcttttc gctgacaccg   2880 

ccgattcttc cgacgcaggt cgctgaatcg atggacaccg tccaccaaga tgatcagcac   2940 

gagcacgccc agcggcttgt cacgtgcttg agcacgtccc gcgacggcat tcagcactca   3000 

gcaattcccg cgccgtgctt gcatggagag actggtaagg gcggccagcg tgagtttcat   3060 

ggcactaacc tttatgtatg tacttacttt tagttgctag tagggatatg gtgacgcctt   3120 

catcctacga aacaagtgaa gactg atg atc gcc atc aca ggt gcc tcc gga     3172 
                            Met Ile Ala Ile Thr Gly Ala Ser Gly 
                              1               5 

caa ctt ggt cgg ttg act ata gag gcg cta ctg aag cgc ctg cca gca     3220 
Gln Leu Gly Arg Leu Thr Ile Glu Ala Leu Leu Lys Arg Leu Pro Ala 
 10                  15                  20                  25 

tcc gaa att att gcc ctc gtc cgg gat ccg aat aag gcc gga gac ctt     3268 
Ser Glu Ile Ile Ala Leu Val Arg Asp Pro Asn Lys Ala Gly Asp Leu 
                 30                  35                  40 

acc gca cgt ggc atc gtg gtg cgc cag gcc gat tac aac cgg ccg gaa     3316 
Thr Ala Arg Gly Ile Val Val Arg Gln Ala Asp Tyr Asn Arg Pro Glu 
             45                  50                  55 

aca ctc cac cgg gcc ctg att ggg gtc aac cgg ttg ctg ttg att tcc     3364 
Thr Leu His Arg Ala Leu Ile Gly Val Asn Arg Leu Leu Leu Ile Ser 
         60                  65                  70 

tcc agt gag gtg ggt caa cga act gcg caa cac cgg gca gtg atc gac     3412 
Ser Ser Glu Val Gly Gln Arg Thr Ala Gln His Arg Ala Val Ile Asp 
     75                  80                  85 

gct gcg aag caa gaa ggt atc gag ttg ctg gct tat acg agt ctg ctt     3460 
Ala Ala Lys Gln Glu Gly Ile Glu Leu Leu Ala Tyr Thr Ser Leu Leu 
 90                  95                 100                 105 

cat gcc gat aaa tcg gcg ctg ggc cta gcg act gaa cac cga gac acg     3508 
His Ala Asp Lys Ser Ala Leu Gly Leu Ala Thr Glu His Arg Asp Thr 
                110                 115                 120 

gaa cag gcc ctg aca gag tcc ggt att cct cat gtc ctg ttg cgc aac     3556 
Glu Gln Ala Leu Thr Glu Ser Gly Ile Pro His Val Leu Leu Arg Asn 
            125                 130                 135 

ggt tgg tat cac gag aac tac acg gcg ggc atc cca gtc gcg ctg gtt     3604 
Gly Trp Tyr His Glu Asn Tyr Thr Ala Gly Ile Pro Val Ala Leu Val 
        140                 145                 150 

cat ggc gtg ttg ctg ggc tgt gcc cag gat ggc ttg att gct tct gct     3652 
His Gly Val Leu Leu Gly Cys Ala Gln Asp Gly Leu Ile Ala Ser Ala 
    155                 160                 165 

gca cgt gct gac tac gcc gaa gca gcg gct gtg gtg ctc acc ggt gag     3700 
Ala Arg Ala Asp Tyr Ala Glu Ala Ala Ala Val Val Leu Thr Gly Glu 
170                 175                 180                 185 

aat cag gca ggt cgc gtc tac gag ctg gcc ggt gaa ccg gca tat acg     3748 
Asn Gln Ala Gly Arg Val Tyr Glu Leu Ala Gly Glu Pro Ala Tyr Thr 
                190                 195                 200 

ctc acc gaa ctg gca gct gag gtg gcg ccg caa gca gga aag acc gtc     3796 
Leu Thr Glu Leu Ala Ala Glu Val Ala Pro Gln Ala Gly Lys Thr Val 
            205                 210                 215 

gtg tat tcg aac cta tcc gag agc gat tac cga tct gcg ttg atc agt     3844 
Val Tyr Ser Asn Leu Ser Glu Ser Asp Tyr Arg Ser Ala Leu Ile Ser 
        220                 225                 230 

gcg ggc ctt ccc gat ggt ttt gcg gca ttg ctc gca gac tct gat gca     3892 
Ala Gly Leu Pro Asp Gly Phe Ala Ala Leu Leu Ala Asp Ser Asp Ala 
    235                 240                 245 

ggc gca gcc aag ggg tat ttg ttt gat tcc agt gga gac agt cgc aag     3940 
Gly Ala Ala Lys Gly Tyr Leu Phe Asp Ser Ser Gly Asp Ser Arg Lys 
250                 255                 260                 265 

ctg atc ggt cgc cca acc act ccg atg tcg gaa gcc atc gcg gca gca     3988 
Leu Ile Gly Arg Pro Thr Thr Pro Met Ser Glu Ala Ile Ala Ala Ala 
                270                 275                 280 

att ggc cgc taaaactgca ttttcgcgac ttgagtgaca cctgggttag             4037 
Ile Gly Arg 

ataacccagg tgtctcgcac cgctttgggt tagtggtggg caatagcggt gtctggtcac   4097 

cgcttgcccg gcggcgcgcc cgctattgga tgattctcaa cttcctggtg ccggcgtctt   4157 

gttggggccc aaacaggcgg gcataacgca atgtggcatt tgcactgtcg cgcatgatgg   4217 

cttctgctcg agcaccttgc ccgctaatca gcgcgtctac cacagcatga tgctgcatgt   4277 

tggcaaaatt gaaccggcgg tactcttggg gaggttgcta ccgtcgacgg ccagtgaact   4337 

gacagaggca aagggcaggt gttcattccg agccaatgct tcacctatgg cagcgttacc   4397 

gctggcatcc acgatagctt gatggaagcg cttgttgatg tcgtggtatt cggcgaggtc   4457 

gtcttcgctg acataacctt tctcaaatag ggcatcgccc tgggccaagc actgcaagag   4517 

gatctcttgc gtttcactgg atagccctcg ctcggcagcc tgccttgcgg ccagtccttc   4577 

aagtacccct cgaacctcca ccgcgcctgc caggtcattt ggggtcattt gccgcactgc   4637 

atagccacgt gcgccttggc gatcagtaac ccttcctgtt ctagcgctcg gaacgcaatg   4697 

cggataggtg tgcgccgaca ctcccaggcg ctcggcagtg gggatttcgg cgatgcgctc   4757 

tcctgccggg agttcgccat ccacaatcat tttgcgcagt agattgagta ctcgctgccc   4817 

gggcccgctc atttcagcct ccgattggat ccagtaatgg tttgagagaa ttttactcgc   4877 

aagggatttc tgggcaatag ccccgctgat tgctggtttt tgtatgtggc gtgcgactat   4937 

cgcacagaat tggatccacc ttggcgcaaa aaaactggag ctacctcatc ggtcgtggtt   4997 

atattggatc ccataaggtc aagttcatag ctgattttgg ctttagatgt ccattgtgga   5057 

tccaaaaaca agatcgccat tgaggaacgc gccatgtttc cgaaaaacgc ctggtatgtc   5117 

gcttgcactc cggatgaaat cgcagataag ccgctaggcc gtcagatctg caacgaaaag   5177 

attgtcttct atcgggggcc ggaaggacgt gttgccgcgg tagaggattt ctgccctcat   5237 

cgcggggcac cgttgtccct gggtttcgtt cgcgacggta agctgatttg cggctaccac   5297 

ggtttggaaa tgggctgcga gggcaaaacg ctcgcgatgc ccgggcagcg cgttcaaggc   5357 

ttcccttgca tcaaaagcta cgcggtagaa gagcgatacg gctttatctg ggtatggcct   5417 

ggtgatcgcg agctggcgga tccggcgctt attcaccacc tggagtgggc cgataatccg   5477 

gagtgggcct atggtggcgg tctctaccac atcgcttgtg attaccgcct gatgatcgac   5537 

aacctcatgg atctcaccca tgagacctat gtgcatgcct ccagcatcgg tcaaaaggaa   5597 

attgacgagg caccggtcag tactcgtgtc gagggcgaca ccgtgattac cagccggtac   5657 

atggataacg tcatggcccc tccgttctgg cgtgctgcgc ttcgtggcaa cggcttggcc   5717 

gacgatgtac cggttgatcg ctggcagatc tgccgattcg ctcctccgag tcacgtactg   5777 

atcgaagtag gtgtggctca tgcgggcaaa ggcggatatg acgcgccggc ggaatacaag   5837 

gccggcagca tagtggtcga cttcatcacg ccggagagtg atacctcgat ttggtacttc   5897 

tggggcatgg ctcgcaactt ccgtccgcag ggcacggagc tgactgaaac cattcgtgtt   5957 

ggtcagggca agatttttgc cgaggacctg gacatgctgg agcagcagca gcgcaatctg   6017 

ctggcctacc cggagcgcca gttgctcaag ctgaatatcg atgccggcgg ggttcagtca   6077 

cggcgcgtca ttgatcggat tctcgcagct gaacaagagg ccgcagacgc agcgctgatc   6137 

gcgagaagtg catcatgatt gaggtaatca tttcggcgat gcgcttggtt gctcaggaca   6197 

tcattagcct tgagtttgtc cgggctgacg gtggcttgct tccgcctgtc gaggccggcg   6257 

cccacgtcga tgtgcatctt cctggcggcc tgattcggca gtactcgctc tggaatcaac   6317 

caggggcgca gagccattac tgcatcggtg ttctgaagga cccggcgtct cgtggtggtt   6377 

cgaaggcggt gcacgagaat cttcgcgtcg ggatgcgcgt gcaaattagc gagccgagga   6437 

acctattccc attggaagag ggggtggagc ggagtctgct gttcgcgggc gggattggca   6497 

ttacgccgat tctgtgtatg gctcaagaat tagcagcacg cgagcaagat ttcgagttgc   6557 

attattgcgc gcgttcgacc gaccgagcgg cgttcgttga atggcttaag gtttgcgact   6617 

ttgctgatca cgtacgtttc cactttgaca atggcccgga tcagcaaaaa ctgaatgccg   6677 

cagcgctgct agcggccgag gccgaaggta cccaccttta tgtctgtggg cccggcgggt   6737 

tcatggggca tgtgcttgat accgcgaagg agcagggctg ggctgacaat cgactgcatc   6797 

gagagtattt cgccgcggcg ccgaatgtga gtgctgacga tggcagtttc gaggtgcgga   6857 

ttcacagcac cggacaagtg cttcaggtcc ccgcggatca aacggtctcc caggtgctcg   6917 

atgcggccgg aattatcgtt cccgtttctt gtgagcaggg catctgcggt acttgcatca   6977 

ctcgggtggt agacggagag cctgatcatc gtgacttctt cctcacggat gcggagaagg   7037 

caaagaacga ccagttcacc ccctgttgct cgcgagccaa gagcgcctgt ttggtcttgg   7097 

atctctaact catccccgtg tccggtcccc tgctttggtg cggcggactg tgcgcgggta   7157 

agtaaacagg ctcaaccgtt tttagcggga taaccattct tgaggatgaa ggagggttat   7217 

cccgctcttt tcatgcacca agccattcat agtcaccagc tgcttctacg tgctgctgcg   7277 

ttacaagttt attcagaagg aaatcggaat gatcaaatcc cgcgccgctg tggcgttcgc   7337 

acccaatcag ccattgcaga tcgtcgaagt ggacgtggct ccgcccaagg ccggtgaagt   7397 

cctggtgcgg gtcgtggcca ccggcgtttg ccacaccgat gcctacaccc tgtccggcgc   7457 

tgattccgag ggcgttttcc cctgcatcct tggtcacgaa ggcggcggca ttgtcgaagc   7517 

ggtgggcgag ggcgtcacct cgctggcggt cggcgaccac gtgatcccgc tctacacggc   7577 

cgaatgccgt gagtgcaagt tcttcaagtc cggcaagacc aacctgtgcc agaaagtgcg   7637 

tgctactcag ggcaagggtc tgatgccgga cggcacctcc cgcttcagct acaacggtca   7697 

gccgatctac cactacatgg gctgctcgac cttctccgag tacaccgtgc tgccggaaat   7757 

ctccctggcg aagattccca agaatgcgcc gctggagaaa gtctgcctgc tgggctgcgg   7817 

cgtgaccacc ggcattggcg cggtgctgaa cactgccaag gtggaggagg gtgctaccgt   7877 

ggccatcttc ggcctgggcg gcatcggctt ggcggcgatc atcggcgcga agatggccaa   7937 

ggcctcgcgc atcatcgcca tcgacatcaa tccgtccaag ttcgatgtgg ctcgcgagct   7997 

gggcgccact gacttcgtca atccgaacga tcacgcgaag ccgatccagg atgtcatcgt   8057 

cgagatgact gatggcggtg tggactacag cttcgagtgc atcggcaacg ttcgactcat   8117 

gcgcgcagca ctcgagtgct gccacaaggg ctggggcgaa tccgtgatca tcggcgtggc   8177 

gccggcgggg gccgaaatca acacccgtcc gttccacctg gtgaccggtc gcgtctggcg   8237 

gggttcggcg ttcggtggcg taaagggccg caccgaactg ccgagctacg tggagaaggc   8297 

acagcagggc gagatcccgc tggacacctt catcactcac accatgggcc tggacgacat   8357 

caacacggcc ttcgacctga tggacgaagg gaagagcatc cgctctgttg ttcaattgag   8417 

tcgctagtga agtggggtga ggaaattgga ttaggaggcg gatggttcct gccgcttaac   8477 

caccttgtcc cagcttctgg ctgagatttc caagattcgg tgaaatttgc catgccgcaa   8537 

actcttgctg gacggttgag tctgttatcc ggcaccgacg aattaaccct gcttcttcgg   8597 

ggtggtcggg gcattgagcg tgaagccttg cgggtcgatg ttcaaggtga actggcgctg   8657 

acgcctcacc cggcggcgct tggctctgcg ttgacccatc cgacaattac tacggattac   8717 

gccgaggccc tgcttgagtt gatcactcgg ccggcaaccg attgtgcgca agccttggct   8777 

gagctggagg agcttcaccg tttcgttcat tcgagacttg agggggagta tctctggaat   8837 

ctgtccatgc ctggcagatt gccggttgat gagcaaatcc cgattgcttg gtatggacca   8897 

tcaaatccag gcatgttgcg ccacgtttat cgccgtggcc tagctctgcg ttatggcaag   8957 

cgaatgcaat gcatcgcagg gattcactac aactactcac tgccgccaga gcttttcgct   9017 

gtcctgacca aggcagaggt cgggtctccc aagttactgg agcgccagtc agcagcttac   9077 

atgcgccaaa ttcgcaacct tcggcaatac ggttggttgc tggcctactt gttcggcgct   9137 

tcccccgcca tctgcaagag cttcttgggg ggcgagagag atgagctagc tcgcatgggg   9197 

ggcgatacgc tttacatgcc ctatgcaacc agcttgcgca tgagtgacat cgggtaccgc   9257 

aaccgtgcca tggatgatct atctcccagc ctgaatgatc tgggtgccta tattcgcgat   9317 

atttgccgtg ctcttcacac tcccgatgcc cagtaccagg cgctgggtgt gtttgcacag   9377 

ggcgagtggc ggcagttaaa cgccaatcta ttgcagttgg atagtgagta ctacgcactg   9437 

gcgcgaccga agtcagcgcc cgagcggggg gagcgaaacc tggatgctct cgctaggcgt   9497 

ggagtccagt atgtggagct gcgcgcactg gatctcgatc cattctcccc gttaggcatt   9557 

ggcctgacct gcgccaagtt cctcgatggc tttttgcttt tctgcttgtt gtctgaggcg   9617 

ccggttgatg atcgaaatgc ccagcgttca agaccgggaa aatctgagcc tggccggcaa   9677 

gtacgggcgt cacctggctt aaagctgcat cggaatggtc agtccattct cctcaaggat   9737 

tgggcgcagg aagtgttgac ggaggttcag gcctgtgtgg aattgctcga cagtgcaaat   9797 

gggggctcat ctcacgcatt ggcttggtca gcacaggagg aaaaggtgct taatccggat   9857 

tgtgcgccat cagctcaggt gctcgcagag atacacagac acggtgggag cttcacggca   9917 

tttggtcgcc aattagctat cgaccatgca aaacacttca gtgcctcctc gcttgaggct   9977 

ggcgtagcca aagcgcttga cctccaggcg acgtcgtctc tgcgcgagca gcatcaattg  10037 

gaggccaacg accgtgcgcc attttctgac taccttcagc aattctccct ggctttcggt  10097 

caatccgtcg gcgcctctcg tgcgcccaac cctaccgcgc acctcatcga tctgacccct  10157 

cctgtctaag gttgtcgtgg gagcagatcc gtgggccgag cttcctccag ggcctggccg  10217 

cagcgatcca gttgctaggt ccctatgctc ttgcataggg taaaaattag ttattgtgtt  10277 

taacgaaacg tctggcatac tggctttagg cacgagcttc cacgccgaag ttgagagcgt  10337 

catgaacgat ttttcgtgtg gagagacgat gcccgatgcg gtcgacgagg ttcaggtcct  10397 

aatggcagtg ccggcggcta aacggaacgt gccgtatttt gaggcttgga gcgtggtgaa  10457 

gcagcttggc tgctccctgg gcctgtcagg atcacgctgt gtcggcagtg acacttcaaa  10517 

acaagaaggg cattaagatg atgaatgtta attataaggc tgtcggggcg agcctactcc  10577 

tcgccttcat ctctcaggga gcttgggcag agagccccgc agcctctggc aatacccctg  10637 

acatttatcg aaagacctgc acctactgcc atgagcctac tgtcaacaat ggccgggtca  10697 

ttgcccgaag cctcgggccg actctgcgag ggcgccagat ccctccacag tacacggagt  10757 

acatggtgcg tcatggacgc ggggcaatgc ctgcattctc tgaagcagaa gtgcctccgg  10817 

cggagctgaa agttctgggc gattggattc agcaaagcag tgctcccaaa gacgctggag  10877 

tcgcgccatg actacccgtc gcaactttct aataggcgcg tcgcaggtgg gggcattggt  10937 

gatgatgtcg ccgaaattgg tcttccgtac gccgctcaag cagaagcccg tgcgcatcct  10997 

gtcgaccggg ctggccggtg agcaagagtt tcactcgatg cttcgcgcgc gattgaccca  11057 

tacgggtcag gtcgacatcg cgtcggtacc gctggacgca gctatttggg cttctcccgc  11117 

tcgacttgcc caggcaatgg atgcgttgaa tggtacgcgt ctgatcgctt ttgttgagcc  11177 

caggaacgaa ttgatactga tgcaattctt gatggatcgc ggggctgcgg tgcttattca  11237 

aggtgagcat gcggtggaca gcaagggggt ctctcggcac gactttctga gtaccccatc  11297 

cagtgcggga attggagggg cgctagccga cagcctggca aaagggggct cgccgttctc  11357 

tatttccgtc cgagcgcttg gctcggtaac tgctcagcca agaagtaatc agagtgaggt  11417 

ggccacccac tggacgaccg ctctggggac ctattatgcc gatatcgcag tggggcgctg  11477 

ggagccgcag cgcgaagtgg ccagctatgg aagtggacta atcatggcgg aacggcttga  11537 

tcgtgttgcc tcaaccttca ttgcagatct ctgagtcagg gtattgatat ggaaagcacc  11597 

gtagttcttc ccgagggtgt caccccggag cagttcacca aagccatcag cgagttccgt  11657 

caggtattgg gtgaggacag tgttcttgtc actgctgaac gagttgttcc ctatacgaaa  11717 

ctcctcattc ctacacagga tgatgcccag tacaccccgg ccggtgcctt gactccttct  11777 

tcggtggagc aggtccagaa agtcatgggg atctgcaata agtacaagat cccggtatgg  11837 

ccaatctcta ccggtcggaa ctgggggtat gggtccgctt cgcctgcaac tcctgggcag  11897 

atgattcttg accttcgcaa gatgaacaag atcattgaga tcgatgttga ggggtgtact  11957 

gccctgctcg agccgggcgt tacctaccag cagcttcacg attacatcaa ggagcacaat  12017 

ctgcccttga tgctggatgt gccgactatt gggcctatgg ttggcccggt gggtaacacg  12077 

ctggatcgag gcgttggtta tacgccgtac ggcgagcact tcatgatgca gtgtggtatg  12137 

gaagtcgtca tggccgatgg cgaaatcctc cgtactggta tgggctcggt gcccaaagcc  12197 

aagacttggc aggcattcaa atggggctat ggtccatatc tggacggtat ctttacccag  12257 

tccaactttg gtgttgtgac aaagctcggg atttggttga tgcccaagcc gccagtgatc  12317 

aagtcgttta tgatccgtta tcccaatgaa gctgatgtgg ttaaggcaat tgatgctttt  12377 

cgcccgctgc gtattactca gctgattcct aacgtcgttt tgttcatgca cggcatgtac  12437 

gaaacggcaa tctgccggac gcgtgctgag gttacttcgg acccaggtcc tatttctgaa  12497 

gcggacgccc gcaaagcatt caaagagcta ggcgttggct actggaacgt ttacttcgcg  12557 

ctttacggca cagaagagca gatagccgtc aatgaaaaga tcgtccgcgg catcctcgaa  12617 

ccgacggggg gtgagatcct caccgaagag gaggctggag ataacattct tttccatcac  12677 

cataagcagc tcatgaacgg cgagatgaca ttggaggaaa tgaatatcta ccagtggcgc  12737 

ggagcaggtg gcggtgcttg ctggtttgca ccggttgctc aggtcaaggg gcatgaggca  12797 

gagcagcagg tcaagcttgc tcagaaggtg cttgcaaagc atgggttcga ttacacggcg  12857 

ggctttgcga ttggttggcg cgatcttcac catgtgatcg atgtgctgta cgaccgtagc  12917 

aatgccgacg agaaaaagcg cgcttacgct tgctttgatg aattgatcga cgtctttgcg  12977 

gccgaaggct ttgcaagtta caggaccaat attgccttta tggacaaagt cgcctctaag  13037 

ttcggcgctg agaataagag ggtcaatcag aagatcaagg ctgcccttga tccaaacggc  13097 

atcatcgctc ccggcaagtc gggcattcat cttcccaaat aatgcgtgtt cgtgaggcgg  13157 

ctgctagccg cctcatttga agaaagagtc gtatcggcga tgcatgatgc gtcgttcgct  13217 

ctcggctgtt gattcttcga aagaagcgta tgggggggga atgattgcaa tcactgcggg  13277 

caccggaagt cttggtcggg ctatcgttga gcgactaggg gactgcggtc ttatcggtca  13337 

agttcgattg acggctcgcg atcctaaaag gcttcgtgcc gctgccgagg aagggtttca  13397 

ggtcgctaag gcggattacg ccgatattgg gagtcttgac caggcattac agggggtaga  13457 

cgtattactc ctgatttctg gtactgcacc caatgaaata aggatccaac agcataagtc  13517 

ggtcatcgac gcggcaaaac gaaacggcgt gtcgcgtatt gtgtatacca gcttcataaa  13577 

tccaagtact cgcagcaggt ctatttgggc ctccattcat cgtgaaactg agacttacct  13637 

caggcagtct ggggtgaagt ttacgattgt ccgaaataat cagtatgcgt ctaacctgga  13697 

tctgttgctg ctgagggctc aagacagcgg aatatttgcc attcccgggg cgaaggggcg  13757 

ggtggcgtac gtctctcatc gcgacgttgc cgctgccatc tgtagtgtcc tgacgaccgc  13817 

cggacacgat aacaggatct accagctcac aggctctgag gctctcaatg ggctcgagat  13877 

cgcggagatt cttggtgggg tgctcgggcg tccagtgcgc gcgatggatg cctcgcctga  13937 

cgagtttgct gccagctttc gcgaggctgg attccctgag tttatggttg aaggcctact  13997 

aagcatttat gccgcttcag gtgctgggga gtaccaatcc gtcagtcctg atgttgggtt  14057 

gttgacggga cgacgtgccg aatcgatgcg aacttacata cagcgtctag tttggccttg  14117 

agggaggtga ccgacgtatg aaggcttatg agcttcacaa gatttcggaa caggtagagg  14177 

tcaggctcca gccaactcgg ccccgcccgc agttgaatca tggcgaggtc ctcatcaggg  14237 

tccatgcagc ctcgctcaac tttcgcgatt tgatgatctt ggccggtcgc tatccgggtc  14297 

aaatgaaacc cgatgtgatc ccgctgtccg atggtgctgg cgagattgtg gaggtcgggc  14357 

ctggcgtatc ttcggaggtg cagggtcagc gcgtagccag cacctttttc cctaactggc  14417 

gggccggaaa gattaccgag ccggctattg aggtgtcgtt gggcttcggt atggacggga  14477 

tgctcgcgga atacgttgct ctgccctatg aggcaacgat accgataccg gagcacctgt  14537 

cgtacgagga ggctgcaaca ttgccttgcg cggcgctaac cgcttggaat gcgttgaccg  14597 

aagtggggcg tgtcaaggcc ggtgatacgg tcttgttgct tggcactggc ggtgtctcga  14657 

tgttcgcgtt gcagttcgcc aagctcttgg gggcgacggt cattcacacc tcgagcagtg  14717 

aacaaaagct ggagagggtg aaagcgatgg gggctgatca tctgatcaac taccgcaatt  14777 

cgccagggtg ggaccgtact gtcctggatc tcaccgcggg gcgaggggtt gacctggtag  14837 

tcgaggtagg gggggcgggg accttggagc gctcacttcg tgcggtcaag gtaggcggta  14897 

ttgtcgccac gattgggcta gtggctggcg ttggcccgat tgacccattg ccgcttatct  14957 

ccagggctat tcagctctcg ggcgtctatg tcggttcccg ggaaatgttt ctctcaatga  15017 

acaaagccat tgcatcagcc gaaatcaagc cagtgatcga ttgctgcttc cccatcgacg  15077 

aggttggaga tgcttatgag tacatgcgta gcggcaatca ccttggcaaa gtagttatca  15137 

cgatctaact gccgctaaac ccgttgtgcg gcaatttgcg ggagctagta ccgggctttc  15197 

ggtttggctc ttggatggtc ttcgcatgca cgctttacga agggggccag ggacagacgc  15257 

cccggggcgt aatcaatggc cttgcgtgca ggctctcacc gtcgtgatcg ggattggaaa  15317 

ttcgtgcgag gacagcggcc acgtaccggc gccctgaagg gctggaaggt tggagtttcg  15377 

ttaaggtctg gtacccagca gccatggaga gcggccctta gccggaatgg cagcttgatg  15437 

gttgccacgg gaccagactg gatgtcttga gtgtcgagaa ttaccagatc gctgcgattt  15497 

tcatcgaggc gaccaaccac ggtcagcaag tacccgtcac cttcggcggc ggtcggactt  15557 

ctagggacga aggccggctc ctgggccgcc gaggcttcgc cggagtacca gaggtcgtag  15617 

tcacctcggt ggttgtccca gatgccgagt gagttgtacg cgaatatctt ctcggcctgc  15677 

tgatgcgcaa gtggtttgcg tggatcgtcc acccccataa agccatagcg gttgcattgc  15737 

agggcgaacg aagaatccat gattggcatt tccgcaaaga aatcgtgtag ccgggttcgc  15797 

ttgatctcgt cgctgctgct atcgaggtca atttcccaac gagtcaggcg tggtacggct  15857 

ttctcagggg cgaagggttg gttttgtgag ttggggaagg ggaacggcag gatttcactt  15917 

tccataaggt cgatataaat cttggttccg acttcccaag cattcacaac atgaaatacc  15977 

cagagcgccg gtgccttgag ccagcgaatc agactgccct ggcgcggcgc gagtacgcca  16037 

atgtagctgc ccagttccgg ctcccacata taaattggct gtttcgcctt gaggcgggac  16097 

aggctgttgg tggccggcat aattgggaaa atggaccaat ttcgggtaat ggcaaagtcg  16157 

tgcatgaatg cgccataggg ctgctcaaac caagtttcat gtgtcacctt gccgtgcttg  16217 

tcgacaatgt aataggccat gtctggagtt gcttcgccct tagctgccga accgaagaac  16277 

aacaagtcac ccgtttccgg gtcatatttt ggatgggcgg tgtgggtttg gctggtaact  16337 

tggccgtcgt agtcgaagtg tccgcgagtt tcaagtgtac gaggatccag ttcgtacggt  16397 

aggccgtctt ccttcaccgc cagcaccttg ccgtgatggc taatgatgct tgtattggca  16457 

acggtgcggt ctagtccttt tacactggtg tcgtcggtat aggggtttct gtacatgcca  16517 

aatagcgatt ttcgcgctag tcgttcggcc gtgaatcgag cggttttaac ccagcgactg  16577 

atgaagtcga catgaccatc ttcgaagtgg aaggcagagg ccattccatc tccatctatg  16637 

aaggtgtgga atttttgtgg ggtaacttga ggctctggcg tattacggta gaacgttcca  16697 

tttattgatt ttgggatttc gccgtcaacc tctagatcga acaagtctgc ctctatacgg  16757 

gtggggagaa gtgttcctac taattgcggg tcgttgcggt tgaatctcgc catggcacgg  16817 

tctcctttgt tgttctgaat ggcctaaatg cgcggcttgc cgggttggag tttatgttta  16877 

ggactgaccg gatttcatgt gtgccggtga agtgaagatg tctgtgagtg caatggtggt  16937 

ggtattgaaa atgggccgag gctggcctat tgtttagaat ttcaagaatg acaactattc  16997 

ggtggcggcg tatgtccatt cactctgagg ggatcactct cgcggattcg ccgctgcatt  17057 

gggcgcatac cctgaatgga tcaatgcgta ctcatttcga agtccagcgt cttgagcggg  17117 

gtagaggtgc ctcccttgcc cgatctagat ttggcgcggg tgagctgtac agtgccattg  17177 

caccaagcca ggtacttcgc cacttcaacg accagcgaaa tgctgatgag gctgagcaca  17237 

gctatttgat tcagatacga agtggcgctt tgggcgttgc atccggcgga agaaaggtga  17297 

tcttggcaaa tggtgattgc tccatagttg atagtcgcca agacttcaca ctttcctcga  17357 

actcttcgac ccaaggtgtc gtaatacgct ttccggtgag ttggctggga gcgtgggtgt  17417 

ccaatccgga ggatcttatc gcccgacgag ttgatgctga ggtagggtgg ggtagggcgc  17477 

taagcgcatc ggtttctaat ctagatccat tgcgcatcga cgatttaggt agcaatgtaa  17537 

atggcattgc agagcatgtt gctatgttaa tttcactagc aagttctgcg gttagttctg  17597 

aagatggggg tgtggctctt cggaaaatga gggaagtgaa gagagtactc gagcagagtt  17657 

tcgcagacgc taatctcggg ccggaaagtg tttcaagtca attaggaatt tcgaaacgct  17717 

atttgcatta tgtctttgct gcgtgcggta cgacctttgg tcgcgagctg ttggaaatac  17777 

gcctgggcaa agcttatcga atgctctgtg cggcgagtga ctcgggtgct gtgctgaagg  17837 

tggccatgtc ctcaggtttt tcggattcaa gccatttcag caagaaattt aaggaaagat  17897 

acggtgtttc gcctgtctcc ttggtgaggc aggcttgatt tcccatagcg ttattgcggt  17957 

cgtcgttgca aatgcggacc tgcgtgatca tcaaggctaa gactgccaca ttaggtgtcg  18017 

actcgagcgt ccctctatcc gcctgaccgc gctccgtccc tagtacctag gaaattgagt  18077 

gggcctactt gccagggcca gttggattcg gtgctggtga gcgctgcggg tgacagaatc  18137 

ctgatcgtgg cgatcacgat ggcgataaag ttgcccggtg tcgtagatcg cagggtgacc  18197 

aagacgggga ctcatggcgc ggatcccgcc agtgatgcct tcgcatgacg ccacctctct  18257 

cctccgctca gccttcatgc ctgactaatt aagtcgtata tcaatctggc tctgtgccgc  18317 

attcagttcc tccagctgca ttgtctctcg gcgggagggc attcccctgc attggccaaa  18377 

tgggtcccct tgttcacgac cggacaagcg caccgtgctg cccgttcgtc gtgtgccctg  18437 

tcaaaaagcc tggcgacgaa agggcggcag gccgcatggc cacggctggg cggtaactga  18497 

tgcttgcgtt aatcgttaac cgtttgaaat tccttgccaa atttcggcga gagaatcatg  18557 

cgggtacgcc tttccgtgcg ctttgatctg cgcttccgtg ccttgaatca gaaaaatagt  18617 

taattgacag aactataggt tcgcagtagc ttttgctcac ccaccaaatc cacagcactg  18677 

gggtgcacga tgaatagcta cgatggccgt tggtctaccg ttgatgtgaa ggttgaagaa  18737 

ggtatcgctt gggtcacgct gaaccgcccg gagaagcgca acgcaatgag cccaactctc  18797 

aatcgagaga tggtcgaggt tctggaggtg ctggagcagg acgcagatgc tcgcgtgctt  18857 

gttctgactg gtgcaggcga atcctggacc gcgggcatgg acctgaagga gtatttccgc  18917 

gagaccgatg ctggccccga aattctgcaa gagaagattc gtcgcgaagc gtcgacctgg  18977 

cagtggaagc tcctgcggat gtacaccaag ccgaccatcg cgatggtcaa tggctggtgc  19037 

ttcggcggcg gcttcagccc gctggtggcc tgtgatctgg ccatctgtgc cgacgaggcc  19097 

acctttggcc tgtccgagat caactggggc atcccgccgg gcaacctggt gagtaaggct  19157 

atggccgaca ccgtgggtca ccgcgagtcc ctttactaca tcatgactgg caagacattt  19217 

ggcggtcagc aggccgccaa gatggggctt gtgaaccaga gtgttccgct ggccgagctg  19277 

cgcagtgtca ctgtagagct ggctcagaac ctgctggaca agaaccccgt agtgctgcgt  19337 

gccgccaaaa taggcttcaa gcgttgccgc gagctgactt gggagcagaa cgaggactac  19397 

ctgtacgcca agctcgacca atcccgtttg ctcgatccgg aaggcggtcg cgagcagggc  19457 

atgaagcagt tccttgacga gaaaagcatc aagccgggct tgcagaccta caagcgctga  19517 

taaatgcgcc ggggccctcg ctgcgccccc ggccttccaa taatgacaat aatgaggagt  19577 

gcccaatgtt tcacgtgccc ctgcttattg gtggtaagcc ttgttcagca tctgatgagc  19637 

gcaccttcga gcgtcgtagc ccgctgaccg gagaagtggt atcgcgcgtc gctgctgcca  19697 

gtttggaaga tgcggacgcc gcagtggccg ctgcacaggc tgcgtttcct gaatgggcgg  19757 

cgcttgctcc gagcgaacgc cgtgcccgac tgctgcgagc ggcggatctt ctagaggacc  19817 

gttcttccga gttcaccgcc gcagcgagtg aaactggcgc agcgggaaac tggtatgggt  19877 

ttaacgttta cctggcggcg ggcatgttgc gggaagccgc ggccatgacc acacagattc  19937 

agggcgatgt cattccgtcc aatgtgcccg gtagctttgc catggcggtt cgacagccat  19997 

gtggcgtggt gctcggtatt gcgccttgga atgctccggt aatccttggc gtacgggctg  20057 

ttgcgatgcc gttggcatgc ggcaataccg tggtgttgaa aagctctgag ctgagtccct  20117 

ttacccatcg cctgattggt caggtgttgc atgatgctgg tctgggggat ggcgtggtga  20177 

atgtcatcag caatgccccg caagacgctc ctgcggtggt ggagcgactg attgcaaatc  20237 

ctgcggtacg tcgagtgaac ttcaccggtt cgacccacgt tggacggatc attggtgagc  20297 

tgtctgcgcg tcatctgaag cctgctgtgc tggaattagg tggtaaggct ccgttcttgg  20357 

tcttggacga tgccgacctc gatgcggcgg tcgaagcggc ggcctttggt gcctacttca  20417 

atcagggtca aatctgcatg tccactgagc gtctgattgt gacagcagtc gcagacgcct  20477 

ttgttgaaaa gctggcgagg aaggtcgcca cactgcgtgc tggcgatcct aatgatccgc  20537 

aatcggtctt gggttcgttg attgatgcca atgcaggtca acgcatccag gttctggtcg  20597 

atgatgcgct cgcaaaaggc gcgcggcagg tcgtcggtgg tggcttagat ggcagcatca  20657 

tgcagccgat gctgcttgat caggtcactg aagagatgcg gctctaccgt gaggagtcct  20717 

ttggccctgt tgccgttgtc ttgcgcggcg atggtgatga agaactgctg cgtcttgcca  20777 

acgattcgga gtttggtctt tcggccgcca ttttcagccg tgacgtctcg cgcgcaatgg  20837 

aattggccca gcgcgtcgat tcgggcattt gccatatcaa tggaccgact gtgcatgacg  20897 

aggctcagat gccattcggt ggggtgaagt ccagcggcta cggcagcttc ggcagtcgag  20957 

catcgattga gcactttacc cagctgcgct ggctgaccat tcagaatggc ccgcggcact  21017 

atccaatcta aatcgatctt cgggcgccgc gggcatcatg cccgcggcgc tcgcctcatt  21077 

tcaatctcta acttgataaa aacagagctg ttctccggtc ttggtggatc aaggccagtc  21137 

gcggagagtc tcgaagagga gagtacagtg aacgccgagt ccacattgca accgcaggca  21197 

tcatcatgct ctgctcagcc acgctaccgc agtgtgtcga ttggtcatcc tccggttgag  21257 

gttacgcaag acgctggagg tattgtccgg atgcgttctc tcgaggcgct tcttcccttc  21317 

ccgggtcgaa ttcttgagcg tctcgagcat tgggctaaga cccgtccaga acaaacctgc  21377 

gttgctgcca gggcggcaaa tggggaatgg cgtcgtatca gctacgcgga aatgttccac  21437 

aacgtccgcg ccatcgcaca gagcttgctt ccttacggac tatcggcaga gcgtccgctg  21497 

cttatcgtct ctggaaatga cctggaacat cttcagctgg catttggggc tatgtatgcg  21557 

ggcattccct attgcccggt gtctcctgct tattcactgc tgtcgcaaga tttggcgaag  21617 

ctgcgtcaca tcgtaggtct tctgcaaccg ggactggtct ttgctgccga tgcagcacct  21677 

ttccagcgcg caattgagac cattctgccg gacgacgtgc ccgcaatctt cactcgaggc  21737 

gaattggccg ggcggcgcac ggtgagtttt gacagcctgc tggagcagcc tggtgggatt  21797 

gaggcagata atgcctttgc ggcaactggc cccgatacga ttgccaagtt cttgttcact  21857 

tctggctcta ccaaactgcc taaggcggtg ccgactactc agcgaatgct ctgcgccaat  21917 

cagcagatgc ttctgcaaac tttcccggtt tttggtgaag agccgccggt gctggtggac  21977 

tggttgccgt ggaaccacac cttcggcggc agccacaaca tcggcatcgt gttgtacaac  22037 

ggcggcacgt actaccttga cgacggtaaa ccaaccgccc aagggttcgc cgagacgctt  22097 

cgcaacttga gcgaaatctc tcccactgcg tacctcactg tgccgaaagg ctgggaggaa  22157 

ttagtgggtg cccttgagcg agacagtacc ctgcgcgaac gcttcttcgc tcgcatgaag  22217 

ctgttcttct tcgcggcggc tgggttgtcg caagggatct gggatcgttt ggaccgggtc  22277 

gctgaacagc actgtggtga gcgcattcgc atgatggcgg gtctgggcat gacggagact  22337 

gctccttcct gcacttttac caccggaccg ctgtcgatgg ctggttacat tgggctgcca  22397 

gcgcctggct gcgaggtcaa gctcgttccg gtcgatggga aattggaagg gcgtttccat  22457 

ggtccgcacg tcatgagcgg ctactggcgt gctcctgaac aaaatgccca agcgttcgac  22517 

gaggaaggct attactgctc cggtgatgcc atcaaattgg cagatcctgc cgatcctcag  22577 

aaaggtctga tgtttgacgg tcgaattgct gaagacttca agctgtcctc aggggtattt  22637 

gtcagcgttg ggccattgcg cacgcgggcg gttctggaag gcggctctta cgtcctggac  22697 

gtagtggttg ctgctcctga tcgtgaatgc cttggattgc tcgtgtttcc gcgtcttctc  22757 

gactgccgtg ccttgtcggg gctaggaaaa gaggcgtcgg acgccgaggt gcttgccagt  22817 

gagccggttc gggcctggtt tgctgactgg ctcaaacgac tcaatcgaga agcaactggc  22877 

aatgccagtc gcatcatgtg ggtagggctc ctcgatacgc cgccgtcgat tgataagggc  22937 

gaggtcactg acaagggctc gatcaaccag cgcgctgttt tgcaatggcg gtcggcgaaa  22997 

gttgatgcgc tgtatcgtgg tgaagatcaa tccatgctgc gtgacgaggc cacactgtga  23057 

gttggtcagg gggggcttac tcggcgtttt ccgacactgc gttggttgcg gcagtgcgca  23117 

ccccctggat tgattgcggg ggtgccctgt cgctggtgtc gcctatcgac ttaggggtaa  23177 

aggtcgctcg cgaagttctg atgcgtgcgt cgcttgaacc acaaatggtc gatagcgtac  23237 

tcgcaggctc tatggctcaa gcaagctttg atgcttacct gctcccgcgg cacattggct  23297 

tgtacagcgg tgttcccaag tcggttccgg ccttgggggt gcagcgcatt tgcggcacag  23357 

gcttcgaact gcttcggcag gccggcgagc agatttccca aggcgctgat cacgtgctgt  23417 

gtgtcgcggc agagtccatg tcgcgtaacc ccatcgcgtc gtatacacac cggggcgggt  23477 

tccgcctcgg tgcgcccgtt gagttcaagg attttttgtg ggaggcattg tttgatcctg  23537 

ctccaggact cgacatgatc gctaccgcag aaaacctggc gcgcctgtac ggaatcacca  23597 

ggggagaagc taattcctac gcggtaagca gcttcgagcg cgcattgagg gcgcaagagg  23657 

agaaatggat tgaccaagag atcgtggctg ttacggatga acagttcgat ttagagggct  23717 

acaacagtcg agcaattgaa ctgcctcgga aggcaaaatt gttgatcgtg acagtcatcc  23777 

gcggcctagc agtctttgaa gccctttccc gattgaagcc tgttcattct ggcggggtgc  23837 

agactgcggg caacagctgt gccgtagtgg acggcgccgc ggcggctttg gtggctcgag  23897 

agtcgtctgc gacacagccg gtcttggcta ggatactggc tacctccgta gtcgggatcg  23957 

agcccgagca tatggggctc ggccctgcgc ccgcgattcg cctgctgctt gcgcgtagtg  24017 

atcttagttt gagggatatc gacctctttg agataaacga ggcgcaggcc gcccaagttc  24077 

tagcggtaca gcatgaattg ggtattgagc actcaaaact taatatttgg ggcggggcca  24137 

ttgcacttgg acacccgctt gccgcgaccg gattgcgtct ctgcatgacc ctcgctcacc  24197 

aattgcaagc taataacttt cgatatggaa ttgcctcggc atgcattggt gggggacagg  24257 

ggatggcggt tcttttagag aatccccact tcggttcgtc ctctgcacga agttcgatga  24317 

ttaacagagt tgaccactat ccactgagct aacgggcatc tcctttgttg ctttgaggtg  24377 

gcgcacgaag gagggctcga aaatctctgc taaaaacaag aagaaggaac agggaacatg  24437 

attagtttcg ctcgtatggc agaaagttta ggagtccagg ctaaacttgc ccttgccttc  24497 

gcactcgtat tatgtgtcgg gctgattgtt accggcacgg gtttctacag tgtacatacc  24557 

ttgtcagggt tggtggaaaa gagcgcgata gctggtgagt tgcgggcgaa aattcaggaa  24617 

ctgaaggttc tggagcagcg cgccttattc atcgccgatg aagggtcgct gaagcagcgc  24677 

tcgatcctcc taagtcaggt gatagctgaa gttaatgatg ctatagatat ttttgacttt  24737 

cagcgcggac gatctgagtt acttaaattc gctgcttctt cgcgcgaagc aagttactcc  24797 

attgaggtcg gtagtaacgc tgcggccgat aagttgcagt cgggcgaacc aagtgacgca  24857 

ttgatggttg ccgataaaaa gctgaatgtt gagtatgagc aattgagttc tgctgtgaat  24917 

gcactgatgg ggcatttaat tgaggatcag aatgaaaaag ttccactaat ctactatatg  24977 

cttggcggcg taactttgtt tacgatgctc atgagtgctt attcggtctg gttcatttcg  25037 

cgtcagttag ttccgccatt aaagtcgacg gtgcagcttg ccgagcggat tgcatcaggc  25097 

gacttggctg atgtcgggga cagcaggcgc aaggatgaaa tcggtcagtt gcaaagtgca  25157 

actaggcgga tggcgattgg actgcgtaat ctggtcggtg atattggtca aagtcgtgcg  25217 

caactggttt catcgtccag cgacctttcg gccatctgtg ctcaggctca gattgatgtc  25277 

gagtgccaga agctttcggt cgcccaggtc tctaccgccg tgaacgagtt ggttgaaacc  25337 

gtccaggcaa tagcaaaaag caccgaagag gcagcaacag tcgccgtctt ggccgatgaa  25397 

aaggcacgcg gtggtgaaag tgtcgttaac aaggccgttg atttcattga gcacctctcc  25457 

ggagatatgg cggaactggg agacgcaatg gagcggcttc agaacgacag tgcgcagatc  25517 

aataaggtag tagacgtcat taaggctgtg gcggagcaga ccaatctgct agccctgaat  25577 

gcggcgatag aggcggcccg tgcaggagag cagggcaggg gctttgcggt cgtggcggat  25637 

gaggttcgtg ctttggcgat gcgcacccaa caatcgacca aagaaattga gaggctagtg  25697 

gtttcattgc agcagggaag tgaagctgcg ggcgagttga tgcggcgtgg caaggtccgg  25757 

acgcatgacg tcgttggatt ggcccagcaa gccgcgcgcc gcgctactcg aaattaccca  25817 

gctgtcgccg gcatccaagc gatgaactat cagatcgccg ctggagcaga gcagcaaggg  25877 

gctgctgtgg ttcaaatcaa ccagaatatg cttgaagtgc ataagatggc tgacgagtcc  25937 

gccattaaag cgggacagac catgaagtca tcgaaggagc ttgctcacct cggcagtgcg  25997 

ctacaaaaat ccgttgatcg attccagctg tagcgctccg ggtggctgaa acgcgcattt  26057 

tcgttaaggt cttcagcgcg gtctgctggt gcgtgggccg ctagcctaac tgttgcgctt  26117 

caggctccgc atggatcttg tgcagcagca atagcaattg ttcacgttcg tcatcactca  26177 

gcatcgacgt cgcgtcttgg tcgctctgta ccacgatctt cttcagctct ttgagctgcg  26237 

tctccccagc tttgctgaga aatatcccat aggaacgctt gtccggcttg cagcgcacgc  26297 

gcacagcaag gccgagcttc tcgagcttgt tcagcaaggg aaccagttgt ggtggttcga  26357 

ttgcgagcat ccgcgctagg tcagcctgca taagcccagg gctcgcttcg atgattagaa  26417 

gtgccgacag ctgcgccggg cgtaggtcat atggcgtcag ggcttcaatc aggccctgag  26477 

cgagcttcag ctgtgagccg gcgtaaggca tagccaatca attgattcag gagcgtatcg  26537 

cccggttcta tcagcgggcc gctttcgaaa gtcatggtgt tagccggtag ggtctttttc  26597 

ttggccatgc ttgttgcctg aaccttcgtt gacatagggc agaggtgcgt ttgccgcttc  26657 

gcttcgcgat gaaccgcatc gagatgctga ggtcaggatt tttccttaac tcgcgtaagc  26717 

attctgtcat ttttttggtg gctttgaaca gcctgatgaa aggtggtctc gccctttgag  26777 

gccgattctt gggcgcttgg cggcgtcgaa gcgatgctcc actaccgatt aagataatta  26837 

aaataaggaa accgcatggt ttcttatgtg aatttgtctg gcatactcca gctcaagggc  26897 

aatttttggg ctattggctg agcagttgcc tctatatggt tattcagaat aacaattgac  26957 

tcctcaggag gtcagcgatg agcattcttg gtttgaatgg tgccccggtc ggagctgagc  27017 

agctgggctc ggctcttgat cgcatgaaga aggcgcacct ggagcagggg cctgcaaact  27077 

tggagctgcg tctgagtagg ctggatcgtg cgattgcaat gcttctggaa aatcgtgaag  27137 

caattgccga cgcggtttct gctgactttg gcaatcgcag ccgtgagcaa acactgcttt  27197 

gcgacattgc tggctcggtg gcaagcctga aggatagccg cgagcacgtg gccaaatgga  27257 

tggagcccga acatcacaag gcgatgtttc caggggcgga ggcacgcgtt gagtttcagc  27317 

cgctgggtgt cgttggggtc attagtccct ggaacttccc tatcgtactg gcctttgggc  27377 

cgctggccgg catattcgca gcaggtaatc gcgccatgct caagccgtcc gagcttaccc  27437 

cgcggacttc tgccctgctt gcggagctaa ttgctcgtta cttcgatgaa actgagctga  27497 

ctacagtgct gggcgacgct gaagtcggtg cgctgttcag tgctcagcct ttcgatcatc  27557 

tgatcttcac cggcggcact gccgtggcca agcacatcat gcgtgccgcg gcggataacc  27617 

tagtgcccgt taccctggaa ttgggtggca aatcgccggt gatcgtttcc cgcagtgcag  27677 

atatggcgga cgttgcacaa cgggtgttga cggtgaaaac cttcaatgcc gggcaaatct  27737 

gtctggcacc ggactatgtg ctgctgccgg aagaatcgct ggatagcttt gtcgccgagg  27797 

cgacgcgctt cgtggccgca atgtatccct cgcttctaga taatccggat tacacgtcga  27857 

tcatcaatgc ccgaaatttc gaccgtctgc atcgctacct gactgatgcg caggcaaagg  27917 

gagggcgcgt cattgaaatc aatcctgcgg ccgaagagtt gggggatagt ggtatcagga  27977 

agatcgcgcc cactttgatc gtgaatgtgt cggatgaaat gctggtcttg aacgaggaga  28037 

tctttggtcc gctgctcccg atcaagactt atcgtgattt cgactcggct atcgactacg  28097 

tcaacagcaa gcagcgacca cttgcctcgt acttcttcgg cgaagatgcg gttgagcgtg  28157 

agcaagtgct taagcgtacg gtttcgggcg ccgtggtcgt gaacgatgtc atgagccatg  28217 

tgatgatgga tacgcttcca tttggtggtg tggggcactc ggggatgggg gcatatcacg  28277 

gcatttatgg tttccgaacc ttcagccatg ccaagcctgt tctcgtgcaa agtcctgtgg  28337 

gtgagtcgaa cttggcgatg cgcgcaccct acggagaagc gatccacgga ctgctctctg  28397 

tcctcctttc aacggagtgt tagaaccgtt ggtagtggtt ttggacgggc ccaggagcat  28457 

gcgcttctgg gcccgtttct tgagtattca ttggatagtc acgcgtggta gcttcgagcc  28517 

tgcacagctg atgagcaccc tggaaggcgc gctgtacgcg gacgactggg ttcatcttcg  28577 

ccattcatga cggaactccg ttccccagta ccgcgatgac tattttgcct cttccgatgt  28637 

ccgattccac gccgcctgac gctaagcggg ggcgggggcg cccgcatccc agcccagaca  28697 

gcaacaaatg agtaggctct tggatgccgc ggcggctgag attggtaacg gcaatttcgt  28757 

caatgtgacg atggattcga ttgcccgtgc tgccggcgtc tcaaaaaaaa cgctgtacgt  28817 

cttggtggcg agcaaggaag aactcatttc ccggttagtg gctcgagaca tgtccaacct  28877 

tgagctgctg ctttgtcacg aggttgagtc tgcggaggcc cttcaggatg agttgcgaaa  28937 

ctatctgctg ctctgggcgc gcttgacctt gtcccctctt gctttgggca tttttctgat  28997 

ggccgtgcag gggcgtgaaa gtgccccggg cctggcgaga atctggtatc gagagggggc  29057 

agagcgttgc ctcagcttgc ttcggggatg gttggcaagg atggcaagcc gggagctgat  29117 

cgctcctgga gatatcgact ccgcagtgga gcttatcgat tcgctcctga tctcacagcc  29177 

tttgaaatta tttggcctgg ggatccagag cggctggacc gatgatcaga tcaatcaacg  29237 

ggtcacaatc gctctcgatg cattccgtcg gtgctatgtc gtttagcacc gttctcgcgg  29297 

gctgtggcgg cgtgacctat ttgtctagtg gtcggcgcga aattcgataa gaaagctggg  29357 

cgcgagtgag gccgagccgg cgggcagctt ccgagacatt gcctttcacc tggcccagag  29417 

catggctaat catcgcgtcc tccacttctt gcagcgtcat cgcgctcagg tcctttgagt  29477 

caagcggcga gtcgattgtg ctggtcggtt tggagaagga agtacttggg ctgccagttt  29537 

cctgtggctg attatcttga gcggtggcca ggatgccgct ggccccaatg gagaacatcg  29597 

gttgagtcag tcgttcaccg ctagtgaaga ggtggctcac gtcaatggct ccatcctccg  29657 

gagcgctgat gactccgcgc tccaccaaat tttgaagctc ccggatgttt cctggaaagt  29717 

cgtagccaag cagggcattg gctgcacgtg gagtgaatcc gctgaccacc cggctatgac  29777 

gctgattgaa gcggtgcagg aaataggtca tcaggagggg aatgtcttcc ttcctctctc  29837 

gaagcggcgg gaggtggatc gggtaaacat tgaggcggaa aaaaaggtcc tcgcggaact  29897 

cgccgcgctg gacgcctgcg cgaagatcga cattggttgc ggctaccaca cggacgtcaa  29957 

ccttgagtgt cctgcttccg ccaacccgtt cgacctccga ctcttgcagg gcgcgaagta  30017 

acttcccttg ggccacgagg cttagcgtcc ctatctcgtc aaggaatagt gtgccgcccg  30077 

aagcgcgctc gaaccgtcct gctcgagatt gggtggcgcc ggtaaacgcc ccccgttcga  30137 

cgccgaacaa ctcggactcc atcagggttt cgggaatacg tgcgcaattg accgcaacaa  30197 

acgggccgtc gtgtctgggg ctgatgcggt gaagcatgcg ggcgaacatc tccttgccca  30257 

cacctgattc acccgtaaac agtaccgtcg cctccgtggg tgctacgcgc ttcagcatgt  30317 

ggcaggcagc attgaatgcc gaggaaattc ccaccatgtc gtgttccgat gcagtgcttg  30377 

agtctgcggc ggagtgatgg ggagtgttcc tttgtccctg ctgcgttctt cgtctctgcg  30437 

gcgtgcttgg ttgccgacaa atggttgcgc taagcgccgc caagtcctct tcggcgtctt  30497 

cccattcttc cgctggcttg ccgatcatgc ggcagatctg cgaacccgtg gagcggcatt  30557 

ccacctctcg gtaaaggatg aggcgaccaa ccagcgcgga cgtatagcca atggcataac  30617 

ccgtctgcgt ccagcacgcg ggctcggtgc cgatgccgta gtgcgcaata tgttcatcat  30677 

cttcgctcga atggtgccag aggaattcgc cgtagtaggt ccccaaatcc atgtcgaagt  30737 

cgaagtggat cggctccacg cgtactgcgc cttccagaga gtgcaagttc gggccggcgg  30797 

caaataggga gagcggatcg gcgttgctga agcgctcctt cagaagggcg gcatctttgg  30857 

cgccgcagtg gtaaccggtt cgcagcatga ttccgcgggc gcgggcgaag cccacgcttt  30917 

caattaattc gcgtcgcaat gcacccagtc cgctgctgtg gaggagcagc attcgcgcgc  30977 

cgttcaacca gatgcgtcca tcgccagggc tgaaaaggag ggattcagtg aggtcatgaa  31037 

gggaggggac ggcgcctggc tccaattgct cgatggcgcc gcgattgagt gtcttgggcg  31097 

cggtcttgga gagttcggct agggagataa atttgctggc catggtggcg gcccctgatg  31157 

ggttggatga ttttctgcat tctgcatcat gaaattcatg aaatcatcac ttttcggggg  31217 

gtgggtgcac gggattgaag gttgctagga gagtgcattg ctcgtaagcc caggaagcac  31277 

gcgggtttca ggatggtgca tggaaatggc atgagctttg ctggatatga ttagagacat  31337 

taactatttt ggcggaatgg aagcacgatt cctcgcccgg tagagcggta accgcgacat  31397 

tcaggaccgt aaaaaggaaa gagcatgcaa ctgaccaaca agaaaatcgt cgtcaccgga  31457 

gtgtcctccg gtatcggtgc cgaaactgcc cgcgttctgc gctctcacgg cgccacagtg  31517 

attggcgtag atcgcaacat gccgagcctg actctggatg ctttcgttca ggctgacctg  31577 

agccatcctg aaggcatcga taaggccatc tctcagctgc cggagaaaat tgacggactc  31637 

tgcaatatcg ccggggtgcc cggcactgcc gatcctcagc tcgtcgcaaa cgtgaactac  31697 

ctgggtctaa agtatctgac cgaggcagtc ctgtcgcgca ttcaacccgg tggttcgatt  31757 

gtcaacgtgt cctctgtgct tggcgccgag tggccggccc gccttcagtt gcataaggag  31817 

ctggggagtg ttgttggatt ctccgaaggc caggcatggc ttaagcagaa tccagtggcc  31877 

cccgaattct gctaccagta tttcaaagaa gcactgatcg tttggtctca agttcaggcg  31937 

caggaatggt tcatgaggac gtctgtacgc atgaactgca tcgcccccgg ccctgtattc  31997 

actcccattc tcaatgagtt cgtcaccatg ctgggtcaag agcggactca ggcggacgct  32057 

catcgtatta agcgcccagc atatgccgat gaagtggccg cggtgattgc attcatgtgt  32117 

gctgaggagt cacgttggat caacggcata aatattccag tggacggagg tttggcatcg  32177 

acctacgtgt aagttcgtgg acgccctttg cacgcgcact atatctctat gcagcagctg  32237 

aaagcagctt tggttttgat cggaggtagc gggcggaaag gtgcagaatg tctaaataat  32297 

aaaggattct tgtgaagctt tagttgtccg taaacgaaaa taaaaataaa gaggaatgat  32357 

atgaaagcaa gtagatcagt ctgcactttc aaaatagcta ccctggcagg cgccatttat  32417 

gcagcgctgc caatgtcagc tgcaaactcg atgcagctgg atgtaggtag ctcggattgg  32477 

acggtgcgtt ggggacaaca ccctcaagta tagccttgcc tctcgcctga atgagcaaga  32537 

ctcaagtctg acaaatgcgc cgactgtcaa tggttatatc cggatattca aagtcagggt  32597 

gatcgtaact ttgaccgggg gcttggtatc caatcgtctc gatattctgt cggagcttga  32657 

tgtcagtcgt gactggttgg tg                                           32679 

 
           
             2  
             284  
             PRT  
             Pseudomonas sp.  
           
            2 

Met Ile Ala Ile Thr Gly Ala Ser Gly Gln Leu Gly Arg Leu Thr Ile 
  1               5                  10                  15 

Glu Ala Leu Leu Lys Arg Leu Pro Ala Ser Glu Ile Ile Ala Leu Val 
             20                  25                  30 

Arg Asp Pro Asn Lys Ala Gly Asp Leu Thr Ala Arg Gly Ile Val Val 
         35                  40                  45 

Arg Gln Ala Asp Tyr Asn Arg Pro Glu Thr Leu His Arg Ala Leu Ile 
     50                  55                  60 

Gly Val Asn Arg Leu Leu Leu Ile Ser Ser Ser Glu Val Gly Gln Arg 
 65                  70                  75                  80 

Thr Ala Gln His Arg Ala Val Ile Asp Ala Ala Lys Gln Glu Gly Ile 
                 85                  90                  95 

Glu Leu Leu Ala Tyr Thr Ser Leu Leu His Ala Asp Lys Ser Ala Leu 
            100                 105                 110 

Gly Leu Ala Thr Glu His Arg Asp Thr Glu Gln Ala Leu Thr Glu Ser 
        115                 120                 125 

Gly Ile Pro His Val Leu Leu Arg Asn Gly Trp Tyr His Glu Asn Tyr 
    130                 135                 140 

Thr Ala Gly Ile Pro Val Ala Leu Val His Gly Val Leu Leu Gly Cys 
145                 150                 155                 160 

Ala Gln Asp Gly Leu Ile Ala Ser Ala Ala Arg Ala Asp Tyr Ala Glu 
                165                 170                 175 

Ala Ala Ala Val Val Leu Thr Gly Glu Asn Gln Ala Gly Arg Val Tyr 
            180                 185                 190 

Glu Leu Ala Gly Glu Pro Ala Tyr Thr Leu Thr Glu Leu Ala Ala Glu 
        195                 200                 205 

Val Ala Pro Gln Ala Gly Lys Thr Val Val Tyr Ser Asn Leu Ser Glu 
    210                 215                 220 

Ser Asp Tyr Arg Ser Ala Leu Ile Ser Ala Gly Leu Pro Asp Gly Phe 
225                 230                 235                 240 

Ala Ala Leu Leu Ala Asp Ser Asp Ala Gly Ala Ala Lys Gly Tyr Leu 
                245                 250                 255 

Phe Asp Ser Ser Gly Asp Ser Arg Lys Leu Ile Gly Arg Pro Thr Thr 
            260                 265                 270 

Pro Met Ser Glu Ala Ile Ala Ala Ala Ile Gly Arg 
        275                 280 

 
           
             3  
             1065  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1062)  
               product = “Vanillinsaeure-O-Demethylase” / 
      gene =“vanA” 
             
           
            3 

atg ttt ccg aaa aac gcc tgg tat gtc gct tgc act ccg gat gaa atc       48 
Met Phe Pro Lys Asn Ala Trp Tyr Val Ala Cys Thr Pro Asp Glu Ile 
  1               5                  10                  15 

gca gat aag ccg cta ggc cgt cag atc tgc aac gaa aag att gtc ttc       96 
Ala Asp Lys Pro Leu Gly Arg Gln Ile Cys Asn Glu Lys Ile Val Phe 
             20                  25                  30 

tat cgg ggg ccg gaa gga cgt gtt gcc gcg gta gag gat ttc tgc cct      144 
Tyr Arg Gly Pro Glu Gly Arg Val Ala Ala Val Glu Asp Phe Cys Pro 
         35                  40                  45 

cat cgc ggg gca ccg ttg tcc ctg ggt ttc gtt cgc gac ggt aag ctg      192 
His Arg Gly Ala Pro Leu Ser Leu Gly Phe Val Arg Asp Gly Lys Leu 
     50                  55                  60 

att tgc ggc tac cac ggt ttg gaa atg ggc tgc gag ggc aaa acg ctc      240 
Ile Cys Gly Tyr His Gly Leu Glu Met Gly Cys Glu Gly Lys Thr Leu 
 65                  70                  75                  80 

gcg atg ccc ggg cag cgc gtt caa ggc ttc cct tgc atc aaa agc tac      288 
Ala Met Pro Gly Gln Arg Val Gln Gly Phe Pro Cys Ile Lys Ser Tyr 
                 85                  90                  95 

gcg gta gaa gag cga tac ggc ttt atc tgg gta tgg cct ggt gat cgc      336 
Ala Val Glu Glu Arg Tyr Gly Phe Ile Trp Val Trp Pro Gly Asp Arg 
            100                 105                 110 

gag ctg gcg gat ccg gcg ctt att cac cac ctg gag tgg gcc gat aat      384 
Glu Leu Ala Asp Pro Ala Leu Ile His His Leu Glu Trp Ala Asp Asn 
        115                 120                 125 

ccg gag tgg gcc tat ggt ggc ggt ctc tac cac atc gct tgt gat tac      432 
Pro Glu Trp Ala Tyr Gly Gly Gly Leu Tyr His Ile Ala Cys Asp Tyr 
    130                 135                 140 

cgc ctg atg atc gac aac ctc atg gat ctc acc cat gag acc tat gtg      480 
Arg Leu Met Ile Asp Asn Leu Met Asp Leu Thr His Glu Thr Tyr Val 
145                 150                 155                 160 

cat gcc tcc agc atc ggt caa aag gaa att gac gag gca ccg gtc agt      528 
His Ala Ser Ser Ile Gly Gln Lys Glu Ile Asp Glu Ala Pro Val Ser 
                165                 170                 175 

act cgt gtc gag ggc gac acc gtg att acc agc cgg tac atg gat aac      576 
Thr Arg Val Glu Gly Asp Thr Val Ile Thr Ser Arg Tyr Met Asp Asn 
            180                 185                 190 

gtc atg gcc cct ccg ttc tgg cgt gct gcg ctt cgt ggc aac ggc ttg      624 
Val Met Ala Pro Pro Phe Trp Arg Ala Ala Leu Arg Gly Asn Gly Leu 
        195                 200                 205 

gcc gac gat gta ccg gtt gat cgc tgg cag atc tgc cga ttc gct cct      672 
Ala Asp Asp Val Pro Val Asp Arg Trp Gln Ile Cys Arg Phe Ala Pro 
    210                 215                 220 

ccg agt cac gta ctg atc gaa gta ggt gtg gct cat gcg ggc aaa ggc      720 
Pro Ser His Val Leu Ile Glu Val Gly Val Ala His Ala Gly Lys Gly 
225                 230                 235                 240 

gga tat gac gcg ccg gcg gaa tac aag gcc ggc agc ata gtg gtc gac      768 
Gly Tyr Asp Ala Pro Ala Glu Tyr Lys Ala Gly Ser Ile Val Val Asp 
                245                 250                 255 

ttc atc acg ccg gag agt gat acc tcg att tgg tac ttc tgg ggc atg      816 
Phe Ile Thr Pro Glu Ser Asp Thr Ser Ile Trp Tyr Phe Trp Gly Met 
            260                 265                 270 

gct cgc aac ttc cgt ccg cag ggc acg gag ctg act gaa acc att cgt      864 
Ala Arg Asn Phe Arg Pro Gln Gly Thr Glu Leu Thr Glu Thr Ile Arg 
        275                 280                 285 

gtt ggt cag ggc aag att ttt gcc gag gac ctg gac atg ctg gag cag      912 
Val Gly Gln Gly Lys Ile Phe Ala Glu Asp Leu Asp Met Leu Glu Gln 
    290                 295                 300 

cag cag cgc aat ctg ctg gcc tac ccg gag cgc cag ttg ctc aag ctg      960 
Gln Gln Arg Asn Leu Leu Ala Tyr Pro Glu Arg Gln Leu Leu Lys Leu 
305                 310                 315                 320 

aat atc gat gcc ggc ggg gtt cag tca cgg cgc gtc att gat cgg att     1008 
Asn Ile Asp Ala Gly Gly Val Gln Ser Arg Arg Val Ile Asp Arg Ile 
                325                 330                 335 

ctc gca gct gaa caa gag gcc gca gac gca gcg ctg atc gcg aga agt     1056 
Leu Ala Ala Glu Gln Glu Ala Ala Asp Ala Ala Leu Ile Ala Arg Ser 
            340                 345                 350 

gca tca tga                                                         1065 
Ala Ser 

 
           
             4  
             354  
             PRT  
             Pseudomonas sp.  
           
            4 

Met Phe Pro Lys Asn Ala Trp Tyr Val Ala Cys Thr Pro Asp Glu Ile 
  1               5                  10                  15 

Ala Asp Lys Pro Leu Gly Arg Gln Ile Cys Asn Glu Lys Ile Val Phe 
             20                  25                  30 

Tyr Arg Gly Pro Glu Gly Arg Val Ala Ala Val Glu Asp Phe Cys Pro 
         35                  40                  45 

His Arg Gly Ala Pro Leu Ser Leu Gly Phe Val Arg Asp Gly Lys Leu 
     50                  55                  60 

Ile Cys Gly Tyr His Gly Leu Glu Met Gly Cys Glu Gly Lys Thr Leu 
 65                  70                  75                  80 

Ala Met Pro Gly Gln Arg Val Gln Gly Phe Pro Cys Ile Lys Ser Tyr 
                 85                  90                  95 

Ala Val Glu Glu Arg Tyr Gly Phe Ile Trp Val Trp Pro Gly Asp Arg 
            100                 105                 110 

Glu Leu Ala Asp Pro Ala Leu Ile His His Leu Glu Trp Ala Asp Asn 
        115                 120                 125 

Pro Glu Trp Ala Tyr Gly Gly Gly Leu Tyr His Ile Ala Cys Asp Tyr 
    130                 135                 140 

Arg Leu Met Ile Asp Asn Leu Met Asp Leu Thr His Glu Thr Tyr Val 
145                 150                 155                 160 

His Ala Ser Ser Ile Gly Gln Lys Glu Ile Asp Glu Ala Pro Val Ser 
                165                 170                 175 

Thr Arg Val Glu Gly Asp Thr Val Ile Thr Ser Arg Tyr Met Asp Asn 
            180                 185                 190 

Val Met Ala Pro Pro Phe Trp Arg Ala Ala Leu Arg Gly Asn Gly Leu 
        195                 200                 205 

Ala Asp Asp Val Pro Val Asp Arg Trp Gln Ile Cys Arg Phe Ala Pro 
    210                 215                 220 

Pro Ser His Val Leu Ile Glu Val Gly Val Ala His Ala Gly Lys Gly 
225                 230                 235                 240 

Gly Tyr Asp Ala Pro Ala Glu Tyr Lys Ala Gly Ser Ile Val Val Asp 
                245                 250                 255 

Phe Ile Thr Pro Glu Ser Asp Thr Ser Ile Trp Tyr Phe Trp Gly Met 
            260                 265                 270 

Ala Arg Asn Phe Arg Pro Gln Gly Thr Glu Leu Thr Glu Thr Ile Arg 
        275                 280                 285 

Val Gly Gln Gly Lys Ile Phe Ala Glu Asp Leu Asp Met Leu Glu Gln 
    290                 295                 300 

Gln Gln Arg Asn Leu Leu Ala Tyr Pro Glu Arg Gln Leu Leu Lys Leu 
305                 310                 315                 320 

Asn Ile Asp Ala Gly Gly Val Gln Ser Arg Arg Val Ile Asp Arg Ile 
                325                 330                 335 

Leu Ala Ala Glu Gln Glu Ala Ala Asp Ala Ala Leu Ile Ala Arg Ser 
            340                 345                 350 

Ala Ser 

 
           
             5  
             954  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(951)  
               product = “Vanillin-O-Demethylase”/ gene = “vanB” 
             
           
            5 

atg att gag gta atc att tcg gcg atg cgc ttg gtt gct cag gac atc       48 
Met Ile Glu Val Ile Ile Ser Ala Met Arg Leu Val Ala Gln Asp Ile 
  1               5                  10                  15 

att agc ctt gag ttt gtc cgg gct gac ggt ggc ttg ctt ccg cct gtc       96 
Ile Ser Leu Glu Phe Val Arg Ala Asp Gly Gly Leu Leu Pro Pro Val 
             20                  25                  30 

gag gcc ggc gcc cac gtc gat gtg cat ctt cct ggc ggc ctg att cgg      144 
Glu Ala Gly Ala His Val Asp Val His Leu Pro Gly Gly Leu Ile Arg 
         35                  40                  45 

cag tac tcg ctc tgg aat caa cca ggg gcg cag agc cat tac tgc atc      192 
Gln Tyr Ser Leu Trp Asn Gln Pro Gly Ala Gln Ser His Tyr Cys Ile 
     50                  55                  60 

ggt gtt ctg aag gac ccg gcg tct cgt ggt ggt tcg aag gcg gtg cac      240 
Gly Val Leu Lys Asp Pro Ala Ser Arg Gly Gly Ser Lys Ala Val His 
 65                  70                  75                  80 

gag aat ctt cgc gtc ggg atg cgc gtg caa att agc gag ccg agg aac      288 
Glu Asn Leu Arg Val Gly Met Arg Val Gln Ile Ser Glu Pro Arg Asn 
                 85                  90                  95 

cta ttc cca ttg gaa gag ggg gtg gag cgg agt ctg ctg ttc gcg ggc      336 
Leu Phe Pro Leu Glu Glu Gly Val Glu Arg Ser Leu Leu Phe Ala Gly 
            100                 105                 110 

ggg att ggc att acg ccg att ctg tgt atg gct caa gaa tta gca gca      384 
Gly Ile Gly Ile Thr Pro Ile Leu Cys Met Ala Gln Glu Leu Ala Ala 
        115                 120                 125 

cgc gag caa gat ttc gag ttg cat tat tgc gcg cgt tcg acc gac cga      432 
Arg Glu Gln Asp Phe Glu Leu His Tyr Cys Ala Arg Ser Thr Asp Arg 
    130                 135                 140 

gcg gcg ttc gtt gaa tgg ctt aag gtt tgc gac ttt gct gat cac gta      480 
Ala Ala Phe Val Glu Trp Leu Lys Val Cys Asp Phe Ala Asp His Val 
145                 150                 155                 160 

cgt ttc cac ttt gac aat ggc ccg gat cag caa aaa ctg aat gcc gca      528 
Arg Phe His Phe Asp Asn Gly Pro Asp Gln Gln Lys Leu Asn Ala Ala 
                165                 170                 175 

gcg ctg cta gcg gcc gag gcc gaa ggt acc cac ctt tat gtc tgt ggg      576 
Ala Leu Leu Ala Ala Glu Ala Glu Gly Thr His Leu Tyr Val Cys Gly 
            180                 185                 190 

ccc ggc ggg ttc atg ggg cat gtg ctt gat acc gcg aag gag cag ggc      624 
Pro Gly Gly Phe Met Gly His Val Leu Asp Thr Ala Lys Glu Gln Gly 
        195                 200                 205 

tgg gct gac aat cga ctg cat cga gag tat ttc gcc gcg gcg ccg aat      672 
Trp Ala Asp Asn Arg Leu His Arg Glu Tyr Phe Ala Ala Ala Pro Asn 
    210                 215                 220 

gtg agt gct gac gat ggc agt ttc gag gtg cgg att cac agc acc gga      720 
Val Ser Ala Asp Asp Gly Ser Phe Glu Val Arg Ile His Ser Thr Gly 
225                 230                 235                 240 

caa gtg ctt cag gtc ccc gcg gat caa acg gtc tcc cag gtg ctc gat      768 
Gln Val Leu Gln Val Pro Ala Asp Gln Thr Val Ser Gln Val Leu Asp 
                245                 250                 255 

gcg gcc gga att atc gtt ccc gtt tct tgt gag cag ggc atc tgc ggt      816 
Ala Ala Gly Ile Ile Val Pro Val Ser Cys Glu Gln Gly Ile Cys Gly 
            260                 265                 270 

act tgc atc act cgg gtg gta gac gga gag cct gat cat cgt gac ttc      864 
Thr Cys Ile Thr Arg Val Val Asp Gly Glu Pro Asp His Arg Asp Phe 
        275                 280                 285 

ttc ctc acg gat gcg gag aag gca aag aac gac cag ttc acc ccc tgt      912 
Phe Leu Thr Asp Ala Glu Lys Ala Lys Asn Asp Gln Phe Thr Pro Cys 
    290                 295                 300 

tgc tcg cga gcc aag agc gcc tgt ttg gtc ttg gat ctc taa              954 
Cys Ser Arg Ala Lys Ser Ala Cys Leu Val Leu Asp Leu 
305                 310                 315 

 
           
             6  
             317  
             PRT  
             Pseudomonas sp.  
           
            6 

Met Ile Glu Val Ile Ile Ser Ala Met Arg Leu Val Ala Gln Asp Ile 
  1               5                  10                  15 

Ile Ser Leu Glu Phe Val Arg Ala Asp Gly Gly Leu Leu Pro Pro Val 
             20                  25                  30 

Glu Ala Gly Ala His Val Asp Val His Leu Pro Gly Gly Leu Ile Arg 
         35                  40                  45 

Gln Tyr Ser Leu Trp Asn Gln Pro Gly Ala Gln Ser His Tyr Cys Ile 
     50                  55                  60 

Gly Val Leu Lys Asp Pro Ala Ser Arg Gly Gly Ser Lys Ala Val His 
 65                  70                  75                  80 

Glu Asn Leu Arg Val Gly Met Arg Val Gln Ile Ser Glu Pro Arg Asn 
                 85                  90                  95 

Leu Phe Pro Leu Glu Glu Gly Val Glu Arg Ser Leu Leu Phe Ala Gly 
            100                 105                 110 

Gly Ile Gly Ile Thr Pro Ile Leu Cys Met Ala Gln Glu Leu Ala Ala 
        115                 120                 125 

Arg Glu Gln Asp Phe Glu Leu His Tyr Cys Ala Arg Ser Thr Asp Arg 
    130                 135                 140 

Ala Ala Phe Val Glu Trp Leu Lys Val Cys Asp Phe Ala Asp His Val 
145                 150                 155                 160 

Arg Phe His Phe Asp Asn Gly Pro Asp Gln Gln Lys Leu Asn Ala Ala 
                165                 170                 175 

Ala Leu Leu Ala Ala Glu Ala Glu Gly Thr His Leu Tyr Val Cys Gly 
            180                 185                 190 

Pro Gly Gly Phe Met Gly His Val Leu Asp Thr Ala Lys Glu Gln Gly 
        195                 200                 205 

Trp Ala Asp Asn Arg Leu His Arg Glu Tyr Phe Ala Ala Ala Pro Asn 
    210                 215                 220 

Val Ser Ala Asp Asp Gly Ser Phe Glu Val Arg Ile His Ser Thr Gly 
225                 230                 235                 240 

Gln Val Leu Gln Val Pro Ala Asp Gln Thr Val Ser Gln Val Leu Asp 
                245                 250                 255 

Ala Ala Gly Ile Ile Val Pro Val Ser Cys Glu Gln Gly Ile Cys Gly 
            260                 265                 270 

Thr Cys Ile Thr Arg Val Val Asp Gly Glu Pro Asp His Arg Asp Phe 
        275                 280                 285 

Phe Leu Thr Asp Ala Glu Lys Ala Lys Asn Asp Gln Phe Thr Pro Cys 
    290                 295                 300 

Cys Ser Arg Ala Lys Ser Ala Cys Leu Val Leu Asp Leu 
305                 310                 315 

 
           
             7  
             1119  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1116)  
               product = “Formaldehyd-Dehydrogenase”/ gene =
      “fdh” 
             
           
            7 

atg atc aaa tcc cgc gcc gct gtg gcg ttc gca ccc aat cag cca ttg       48 
Met Ile Lys Ser Arg Ala Ala Val Ala Phe Ala Pro Asn Gln Pro Leu 
  1               5                  10                  15 

cag atc gtc gaa gtg gac gtg gct ccg ccc aag gcc ggt gaa gtc ctg       96 
Gln Ile Val Glu Val Asp Val Ala Pro Pro Lys Ala Gly Glu Val Leu 
             20                  25                  30 

gtg cgg gtc gtg gcc acc ggc gtt tgc cac acc gat gcc tac acc ctg      144 
Val Arg Val Val Ala Thr Gly Val Cys His Thr Asp Ala Tyr Thr Leu 
         35                  40                  45 

tcc ggc gct gat tcc gag ggc gtt ttc ccc tgc atc ctt ggt cac gaa      192 
Ser Gly Ala Asp Ser Glu Gly Val Phe Pro Cys Ile Leu Gly His Glu 
     50                  55                  60 

ggc ggc ggc att gtc gaa gcg gtg ggc gag ggc gtc acc tcg ctg gcg      240 
Gly Gly Gly Ile Val Glu Ala Val Gly Glu Gly Val Thr Ser Leu Ala 
 65                  70                  75                  80 

gtc ggc gac cac gtg atc ccg ctc tac acg gcc gaa tgc cgt gag tgc      288 
Val Gly Asp His Val Ile Pro Leu Tyr Thr Ala Glu Cys Arg Glu Cys 
                 85                  90                  95 

aag ttc ttc aag tcc ggc aag acc aac ctg tgc cag aaa gtg cgt gct      336 
Lys Phe Phe Lys Ser Gly Lys Thr Asn Leu Cys Gln Lys Val Arg Ala 
            100                 105                 110 

act cag ggc aag ggt ctg atg ccg gac ggc acc tcc cgc ttc agc tac      384 
Thr Gln Gly Lys Gly Leu Met Pro Asp Gly Thr Ser Arg Phe Ser Tyr 
        115                 120                 125 

aac ggt cag ccg atc tac cac tac atg ggc tgc tcg acc ttc tcc gag      432 
Asn Gly Gln Pro Ile Tyr His Tyr Met Gly Cys Ser Thr Phe Ser Glu 
    130                 135                 140 

tac acc gtg ctg ccg gaa atc tcc ctg gcg aag att ccc aag aat gcg      480 
Tyr Thr Val Leu Pro Glu Ile Ser Leu Ala Lys Ile Pro Lys Asn Ala 
145                 150                 155                 160 

ccg ctg gag aaa gtc tgc ctg ctg ggc tgc ggc gtg acc acc ggc att      528 
Pro Leu Glu Lys Val Cys Leu Leu Gly Cys Gly Val Thr Thr Gly Ile 
                165                 170                 175 

ggc gcg gtg ctg aac act gcc aag gtg gag gag ggt gct acc gtg gcc      576 
Gly Ala Val Leu Asn Thr Ala Lys Val Glu Glu Gly Ala Thr Val Ala 
            180                 185                 190 

atc ttc ggc ctg ggc ggc atc ggc ttg gcg gcg atc atc ggc gcg aag      624 
Ile Phe Gly Leu Gly Gly Ile Gly Leu Ala Ala Ile Ile Gly Ala Lys 
        195                 200                 205 

atg gcc aag gcc tcg cgc atc atc gcc atc gac atc aat ccg tcc aag      672 
Met Ala Lys Ala Ser Arg Ile Ile Ala Ile Asp Ile Asn Pro Ser Lys 
    210                 215                 220 

ttc gat gtg gct cgc gag ctg ggc gcc act gac ttc gtc aat ccg aac      720 
Phe Asp Val Ala Arg Glu Leu Gly Ala Thr Asp Phe Val Asn Pro Asn 
225                 230                 235                 240 

gat cac gcg aag ccg atc cag gat gtc atc gtc gag atg act gat ggc      768 
Asp His Ala Lys Pro Ile Gln Asp Val Ile Val Glu Met Thr Asp Gly 
                245                 250                 255 

ggt gtg gac tac agc ttc gag tgc atc ggc aac gtt cga ctc atg cgc      816 
Gly Val Asp Tyr Ser Phe Glu Cys Ile Gly Asn Val Arg Leu Met Arg 
            260                 265                 270 

gca gca ctc gag tgc tgc cac aag ggc tgg ggc gaa tcc gtg atc atc      864 
Ala Ala Leu Glu Cys Cys His Lys Gly Trp Gly Glu Ser Val Ile Ile 
        275                 280                 285 

ggc gtg gcg ccg gcg ggg gcc gaa atc aac acc cgt ccg ttc cac ctg      912 
Gly Val Ala Pro Ala Gly Ala Glu Ile Asn Thr Arg Pro Phe His Leu 
    290                 295                 300 

gtg acc ggt cgc gtc tgg cgg ggt tcg gcg ttc ggt ggc gta aag ggc      960 
Val Thr Gly Arg Val Trp Arg Gly Ser Ala Phe Gly Gly Val Lys Gly 
305                 310                 315                 320 

cgc acc gaa ctg ccg agc tac gtg gag aag gca cag cag ggc gag atc     1008 
Arg Thr Glu Leu Pro Ser Tyr Val Glu Lys Ala Gln Gln Gly Glu Ile 
                325                 330                 335 

ccg ctg gac acc ttc atc act cac acc atg ggc ctg gac gac atc aac     1056 
Pro Leu Asp Thr Phe Ile Thr His Thr Met Gly Leu Asp Asp Ile Asn 
            340                 345                 350 

acg gcc ttc gac ctg atg gac gaa ggg aag agc atc cgc tct gtt gtt     1104 
Thr Ala Phe Asp Leu Met Asp Glu Gly Lys Ser Ile Arg Ser Val Val 
        355                 360                 365 

caa ttg agt cgc tag                                                 1119 
Gln Leu Ser Arg 
    370 

 
           
             8  
             372  
             PRT  
             Pseudomonas sp.  
           
            8 

Met Ile Lys Ser Arg Ala Ala Val Ala Phe Ala Pro Asn Gln Pro Leu 
  1               5                  10                  15 

Gln Ile Val Glu Val Asp Val Ala Pro Pro Lys Ala Gly Glu Val Leu 
             20                  25                  30 

Val Arg Val Val Ala Thr Gly Val Cys His Thr Asp Ala Tyr Thr Leu 
         35                  40                  45 

Ser Gly Ala Asp Ser Glu Gly Val Phe Pro Cys Ile Leu Gly His Glu 
     50                  55                  60 

Gly Gly Gly Ile Val Glu Ala Val Gly Glu Gly Val Thr Ser Leu Ala 
 65                  70                  75                  80 

Val Gly Asp His Val Ile Pro Leu Tyr Thr Ala Glu Cys Arg Glu Cys 
                 85                  90                  95 

Lys Phe Phe Lys Ser Gly Lys Thr Asn Leu Cys Gln Lys Val Arg Ala 
            100                 105                 110 

Thr Gln Gly Lys Gly Leu Met Pro Asp Gly Thr Ser Arg Phe Ser Tyr 
        115                 120                 125 

Asn Gly Gln Pro Ile Tyr His Tyr Met Gly Cys Ser Thr Phe Ser Glu 
    130                 135                 140 

Tyr Thr Val Leu Pro Glu Ile Ser Leu Ala Lys Ile Pro Lys Asn Ala 
145                 150                 155                 160 

Pro Leu Glu Lys Val Cys Leu Leu Gly Cys Gly Val Thr Thr Gly Ile 
                165                 170                 175 

Gly Ala Val Leu Asn Thr Ala Lys Val Glu Glu Gly Ala Thr Val Ala 
            180                 185                 190 

Ile Phe Gly Leu Gly Gly Ile Gly Leu Ala Ala Ile Ile Gly Ala Lys 
        195                 200                 205 

Met Ala Lys Ala Ser Arg Ile Ile Ala Ile Asp Ile Asn Pro Ser Lys 
    210                 215                 220 

Phe Asp Val Ala Arg Glu Leu Gly Ala Thr Asp Phe Val Asn Pro Asn 
225                 230                 235                 240 

Asp His Ala Lys Pro Ile Gln Asp Val Ile Val Glu Met Thr Asp Gly 
                245                 250                 255 

Gly Val Asp Tyr Ser Phe Glu Cys Ile Gly Asn Val Arg Leu Met Arg 
            260                 265                 270 

Ala Ala Leu Glu Cys Cys His Lys Gly Trp Gly Glu Ser Val Ile Ile 
        275                 280                 285 

Gly Val Ala Pro Ala Gly Ala Glu Ile Asn Thr Arg Pro Phe His Leu 
    290                 295                 300 

Val Thr Gly Arg Val Trp Arg Gly Ser Ala Phe Gly Gly Val Lys Gly 
305                 310                 315                 320 

Arg Thr Glu Leu Pro Ser Tyr Val Glu Lys Ala Gln Gln Gly Glu Ile 
                325                 330                 335 

Pro Leu Asp Thr Phe Ile Thr His Thr Met Gly Leu Asp Asp Ile Asn 
            340                 345                 350 

Thr Ala Phe Asp Leu Met Asp Glu Gly Lys Ser Ile Arg Ser Val Val 
        355                 360                 365 

Gln Leu Ser Arg 
    370 

 
           
             9  
             1638  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1635)  
               product = “gamma-Glutamylcystein-Synthetase” / 
      gene = “gcs” 
             
           
            9 

atg ccg caa act ctt gct gga cgg ttg agt ctg tta tcc ggc acc gac       48 
Met Pro Gln Thr Leu Ala Gly Arg Leu Ser Leu Leu Ser Gly Thr Asp 
  1               5                  10                  15 

gaa tta acc ctg ctt ctt cgg ggt ggt cgg ggc att gag cgt gaa gcc       96 
Glu Leu Thr Leu Leu Leu Arg Gly Gly Arg Gly Ile Glu Arg Glu Ala 
             20                  25                  30 

ttg cgg gtc gat gtt caa ggt gaa ctg gcg ctg acg cct cac ccg gcg      144 
Leu Arg Val Asp Val Gln Gly Glu Leu Ala Leu Thr Pro His Pro Ala 
         35                  40                  45 

gcg ctt ggc tct gcg ttg acc cat ccg aca att act acg gat tac gcc      192 
Ala Leu Gly Ser Ala Leu Thr His Pro Thr Ile Thr Thr Asp Tyr Ala 
     50                  55                  60 

gag gcc ctg ctt gag ttg atc act cgg ccg gca acc gat tgt gcg caa      240 
Glu Ala Leu Leu Glu Leu Ile Thr Arg Pro Ala Thr Asp Cys Ala Gln 
 65                  70                  75                  80 

gcc ttg gct gag ctg gag gag ctt cac cgt ttc gtt cat tcg aga ctt      288 
Ala Leu Ala Glu Leu Glu Glu Leu His Arg Phe Val His Ser Arg Leu 
                 85                  90                  95 

gag ggg gag tat ctc tgg aat ctg tcc atg cct ggc aga ttg ccg gtt      336 
Glu Gly Glu Tyr Leu Trp Asn Leu Ser Met Pro Gly Arg Leu Pro Val 
            100                 105                 110 

gat gag caa atc ccg att gct tgg tat gga cca tca aat cca ggc atg      384 
Asp Glu Gln Ile Pro Ile Ala Trp Tyr Gly Pro Ser Asn Pro Gly Met 
        115                 120                 125 

ttg cgc cac gtt tat cgc cgt ggc cta gct ctg cgt tat ggc aag cga      432 
Leu Arg His Val Tyr Arg Arg Gly Leu Ala Leu Arg Tyr Gly Lys Arg 
    130                 135                 140 

atg caa tgc atc gca ggg att cac tac aac tac tca ctg ccg cca gag      480 
Met Gln Cys Ile Ala Gly Ile His Tyr Asn Tyr Ser Leu Pro Pro Glu 
145                 150                 155                 160 

ctt ttc gct gtc ctg acc aag gca gag gtc ggg tct ccc aag tta ctg      528 
Leu Phe Ala Val Leu Thr Lys Ala Glu Val Gly Ser Pro Lys Leu Leu 
                165                 170                 175 

gag cgc cag tca gca gct tac atg cgc caa att cgc aac ctt cgg caa      576 
Glu Arg Gln Ser Ala Ala Tyr Met Arg Gln Ile Arg Asn Leu Arg Gln 
            180                 185                 190 

tac ggt tgg ttg ctg gcc tac ttg ttc ggc gct tcc ccc gcc atc tgc      624 
Tyr Gly Trp Leu Leu Ala Tyr Leu Phe Gly Ala Ser Pro Ala Ile Cys 
        195                 200                 205 

aag agc ttc ttg ggg ggc gag aga gat gag cta gct cgc atg ggg ggc      672 
Lys Ser Phe Leu Gly Gly Glu Arg Asp Glu Leu Ala Arg Met Gly Gly 
    210                 215                 220 

gat acg ctt tac atg ccc tat gca acc agc ttg cgc atg agt gac atc      720 
Asp Thr Leu Tyr Met Pro Tyr Ala Thr Ser Leu Arg Met Ser Asp Ile 
225                 230                 235                 240 

ggg tac cgc aac cgt gcc atg gat gat cta tct ccc agc ctg aat gat      768 
Gly Tyr Arg Asn Arg Ala Met Asp Asp Leu Ser Pro Ser Leu Asn Asp 
                245                 250                 255 

ctg ggt gcc tat att cgc gat att tgc cgt gct ctt cac act ccc gat      816 
Leu Gly Ala Tyr Ile Arg Asp Ile Cys Arg Ala Leu His Thr Pro Asp 
            260                 265                 270 

gcc cag tac cag gcg ctg ggt gtg ttt gca cag ggc gag tgg cgg cag      864 
Ala Gln Tyr Gln Ala Leu Gly Val Phe Ala Gln Gly Glu Trp Arg Gln 
        275                 280                 285 

tta aac gcc aat cta ttg cag ttg gat agt gag tac tac gca ctg gcg      912 
Leu Asn Ala Asn Leu Leu Gln Leu Asp Ser Glu Tyr Tyr Ala Leu Ala 
    290                 295                 300 

cga ccg aag tca gcg ccc gag cgg ggg gag cga aac ctg gat gct ctc      960 
Arg Pro Lys Ser Ala Pro Glu Arg Gly Glu Arg Asn Leu Asp Ala Leu 
305                 310                 315                 320 

gct agg cgt gga gtc cag tat gtg gag ctg cgc gca ctg gat ctc gat     1008 
Ala Arg Arg Gly Val Gln Tyr Val Glu Leu Arg Ala Leu Asp Leu Asp 
                325                 330                 335 

cca ttc tcc ccg tta ggc att ggc ctg acc tgc gcc aag ttc ctc gat     1056 
Pro Phe Ser Pro Leu Gly Ile Gly Leu Thr Cys Ala Lys Phe Leu Asp 
            340                 345                 350 

ggc ttt ttg ctt ttc tgc ttg ttg tct gag gcg ccg gtt gat gat cga     1104 
Gly Phe Leu Leu Phe Cys Leu Leu Ser Glu Ala Pro Val Asp Asp Arg 
        355                 360                 365 

aat gcc cag cgt tca aga ccg gga aaa tct gag cct ggc cgg caa gta     1152 
Asn Ala Gln Arg Ser Arg Pro Gly Lys Ser Glu Pro Gly Arg Gln Val 
    370                 375                 380 

cgg gcg tca cct ggc tta aag ctg cat cgg aat ggt cag tcc att ctc     1200 
Arg Ala Ser Pro Gly Leu Lys Leu His Arg Asn Gly Gln Ser Ile Leu 
385                 390                 395                 400 

ctc aag gat tgg gcg cag gaa gtg ttg acg gag gtt cag gcc tgt gtg     1248 
Leu Lys Asp Trp Ala Gln Glu Val Leu Thr Glu Val Gln Ala Cys Val 
                405                 410                 415 

gaa ttg ctc gac agt gca aat ggg ggc tca tct cac gca ttg gct tgg     1296 
Glu Leu Leu Asp Ser Ala Asn Gly Gly Ser Ser His Ala Leu Ala Trp 
            420                 425                 430 

tca gca cag gag gaa aag gtg ctt aat ccg gat tgt gcg cca tca gct     1344 
Ser Ala Gln Glu Glu Lys Val Leu Asn Pro Asp Cys Ala Pro Ser Ala 
        435                 440                 445 

cag gtg ctc gca gag ata cac aga cac ggt ggg agc ttc acg gca ttt     1392 
Gln Val Leu Ala Glu Ile His Arg His Gly Gly Ser Phe Thr Ala Phe 
    450                 455                 460 

ggt cgc caa tta gct atc gac cat gca aaa cac ttc agt gcc tcc tcg     1440 
Gly Arg Gln Leu Ala Ile Asp His Ala Lys His Phe Ser Ala Ser Ser 
465                 470                 475                 480 

ctt gag gct ggc gta gcc aaa gcg ctt gac ctc cag gcg acg tcg tct     1488 
Leu Glu Ala Gly Val Ala Lys Ala Leu Asp Leu Gln Ala Thr Ser Ser 
                485                 490                 495 

ctg cgc gag cag cat caa ttg gag gcc aac gac cgt gcg cca ttt tct     1536 
Leu Arg Glu Gln His Gln Leu Glu Ala Asn Asp Arg Ala Pro Phe Ser 
            500                 505                 510 

gac tac ctt cag caa ttc tcc ctg gct ttc ggt caa tcc gtc ggc gcc     1584 
Asp Tyr Leu Gln Gln Phe Ser Leu Ala Phe Gly Gln Ser Val Gly Ala 
        515                 520                 525 

tct cgt gcg ccc aac cct acc gcg cac ctc atc gat ctg acc cct cct     1632 
Ser Arg Ala Pro Asn Pro Thr Ala His Leu Ile Asp Leu Thr Pro Pro 
    530                 535                 540 

gtc taa                                                             1638 
Val 
545 

 
           
             10  
             545  
             PRT  
             Pseudomonas sp.  
           
            10 

Met Pro Gln Thr Leu Ala Gly Arg Leu Ser Leu Leu Ser Gly Thr Asp 
  1               5                  10                  15 

Glu Leu Thr Leu Leu Leu Arg Gly Gly Arg Gly Ile Glu Arg Glu Ala 
             20                  25                  30 

Leu Arg Val Asp Val Gln Gly Glu Leu Ala Leu Thr Pro His Pro Ala 
         35                  40                  45 

Ala Leu Gly Ser Ala Leu Thr His Pro Thr Ile Thr Thr Asp Tyr Ala 
     50                  55                  60 

Glu Ala Leu Leu Glu Leu Ile Thr Arg Pro Ala Thr Asp Cys Ala Gln 
 65                  70                  75                  80 

Ala Leu Ala Glu Leu Glu Glu Leu His Arg Phe Val His Ser Arg Leu 
                 85                  90                  95 

Glu Gly Glu Tyr Leu Trp Asn Leu Ser Met Pro Gly Arg Leu Pro Val 
            100                 105                 110 

Asp Glu Gln Ile Pro Ile Ala Trp Tyr Gly Pro Ser Asn Pro Gly Met 
        115                 120                 125 

Leu Arg His Val Tyr Arg Arg Gly Leu Ala Leu Arg Tyr Gly Lys Arg 
    130                 135                 140 

Met Gln Cys Ile Ala Gly Ile His Tyr Asn Tyr Ser Leu Pro Pro Glu 
145                 150                 155                 160 

Leu Phe Ala Val Leu Thr Lys Ala Glu Val Gly Ser Pro Lys Leu Leu 
                165                 170                 175 

Glu Arg Gln Ser Ala Ala Tyr Met Arg Gln Ile Arg Asn Leu Arg Gln 
            180                 185                 190 

Tyr Gly Trp Leu Leu Ala Tyr Leu Phe Gly Ala Ser Pro Ala Ile Cys 
        195                 200                 205 

Lys Ser Phe Leu Gly Gly Glu Arg Asp Glu Leu Ala Arg Met Gly Gly 
    210                 215                 220 

Asp Thr Leu Tyr Met Pro Tyr Ala Thr Ser Leu Arg Met Ser Asp Ile 
225                 230                 235                 240 

Gly Tyr Arg Asn Arg Ala Met Asp Asp Leu Ser Pro Ser Leu Asn Asp 
                245                 250                 255 

Leu Gly Ala Tyr Ile Arg Asp Ile Cys Arg Ala Leu His Thr Pro Asp 
            260                 265                 270 

Ala Gln Tyr Gln Ala Leu Gly Val Phe Ala Gln Gly Glu Trp Arg Gln 
        275                 280                 285 

Leu Asn Ala Asn Leu Leu Gln Leu Asp Ser Glu Tyr Tyr Ala Leu Ala 
    290                 295                 300 

Arg Pro Lys Ser Ala Pro Glu Arg Gly Glu Arg Asn Leu Asp Ala Leu 
305                 310                 315                 320 

Ala Arg Arg Gly Val Gln Tyr Val Glu Leu Arg Ala Leu Asp Leu Asp 
                325                 330                 335 

Pro Phe Ser Pro Leu Gly Ile Gly Leu Thr Cys Ala Lys Phe Leu Asp 
            340                 345                 350 

Gly Phe Leu Leu Phe Cys Leu Leu Ser Glu Ala Pro Val Asp Asp Arg 
        355                 360                 365 

Asn Ala Gln Arg Ser Arg Pro Gly Lys Ser Glu Pro Gly Arg Gln Val 
    370                 375                 380 

Arg Ala Ser Pro Gly Leu Lys Leu His Arg Asn Gly Gln Ser Ile Leu 
385                 390                 395                 400 

Leu Lys Asp Trp Ala Gln Glu Val Leu Thr Glu Val Gln Ala Cys Val 
                405                 410                 415 

Glu Leu Leu Asp Ser Ala Asn Gly Gly Ser Ser His Ala Leu Ala Trp 
            420                 425                 430 

Ser Ala Gln Glu Glu Lys Val Leu Asn Pro Asp Cys Ala Pro Ser Ala 
        435                 440                 445 

Gln Val Leu Ala Glu Ile His Arg His Gly Gly Ser Phe Thr Ala Phe 
    450                 455                 460 

Gly Arg Gln Leu Ala Ile Asp His Ala Lys His Phe Ser Ala Ser Ser 
465                 470                 475                 480 

Leu Glu Ala Gly Val Ala Lys Ala Leu Asp Leu Gln Ala Thr Ser Ser 
                485                 490                 495 

Leu Arg Glu Gln His Gln Leu Glu Ala Asn Asp Arg Ala Pro Phe Ser 
            500                 505                 510 

Asp Tyr Leu Gln Gln Phe Ser Leu Ala Phe Gly Gln Ser Val Gly Ala 
        515                 520                 525 

Ser Arg Ala Pro Asn Pro Thr Ala His Leu Ile Asp Leu Thr Pro Pro 
    530                 535                 540 

Val 
545 

 
           
             11  
             354  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(351)  
               product = “Cytochrom C UE-Eugenol-Hydroxylase”
      /gene = “chyA” 
             
           
            11 

atg atg aat gtt aat tat aag gct gtc ggg gcg agc cta ctc ctc gcc       48 
Met Met Asn Val Asn Tyr Lys Ala Val Gly Ala Ser Leu Leu Leu Ala 
  1               5                  10                  15 

ttc atc tct cag gga gct tgg gca gag agc ccc gca gcc tct ggc aat       96 
Phe Ile Ser Gln Gly Ala Trp Ala Glu Ser Pro Ala Ala Ser Gly Asn 
             20                  25                  30 

acc cct gac att tat cga aag acc tgc acc tac tgc cat gag cct act      144 
Thr Pro Asp Ile Tyr Arg Lys Thr Cys Thr Tyr Cys His Glu Pro Thr 
         35                  40                  45 

gtc aac aat ggc cgg gtc att gcc cga agc ctc ggg ccg act ctg cga      192 
Val Asn Asn Gly Arg Val Ile Ala Arg Ser Leu Gly Pro Thr Leu Arg 
     50                  55                  60 

ggg cgc cag atc cct cca cag tac acg gag tac atg gtg cgt cat gga      240 
Gly Arg Gln Ile Pro Pro Gln Tyr Thr Glu Tyr Met Val Arg His Gly 
 65                  70                  75                  80 

cgc ggg gca atg cct gca ttc tct gaa gca gaa gtg cct ccg gcg gag      288 
Arg Gly Ala Met Pro Ala Phe Ser Glu Ala Glu Val Pro Pro Ala Glu 
                 85                  90                  95 

ctg aaa gtt ctg ggc gat tgg att cag caa agc agt gct ccc aaa gac      336 
Leu Lys Val Leu Gly Asp Trp Ile Gln Gln Ser Ser Ala Pro Lys Asp 
            100                 105                 110 

gct gga gtc gcg cca tga                                              354 
Ala Gly Val Ala Pro 
        115 

 
           
             12  
             117  
             PRT  
             Pseudomonas sp.  
           
            12 

Met Met Asn Val Asn Tyr Lys Ala Val Gly Ala Ser Leu Leu Leu Ala 
  1               5                  10                  15 

Phe Ile Ser Gln Gly Ala Trp Ala Glu Ser Pro Ala Ala Ser Gly Asn 
             20                  25                  30 

Thr Pro Asp Ile Tyr Arg Lys Thr Cys Thr Tyr Cys His Glu Pro Thr 
         35                  40                  45 

Val Asn Asn Gly Arg Val Ile Ala Arg Ser Leu Gly Pro Thr Leu Arg 
     50                  55                  60 

Gly Arg Gln Ile Pro Pro Gln Tyr Thr Glu Tyr Met Val Arg His Gly 
 65                  70                  75                  80 

Arg Gly Ala Met Pro Ala Phe Ser Glu Ala Glu Val Pro Pro Ala Glu 
                 85                  90                  95 

Leu Lys Val Leu Gly Asp Trp Ile Gln Gln Ser Ser Ala Pro Lys Asp 
            100                 105                 110 

Ala Gly Val Ala Pro 
        115 

 
           
             13  
             687  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(684)  
               gene = “ORF5” 
             
           
            13 

atg act acc cgt cgc aac ttt cta ata ggc gcg tcg cag gtg ggg gca       48 
Met Thr Thr Arg Arg Asn Phe Leu Ile Gly Ala Ser Gln Val Gly Ala 
  1               5                  10                  15 

ttg gtg atg atg tcg ccg aaa ttg gtc ttc cgt acg ccg ctc aag cag       96 
Leu Val Met Met Ser Pro Lys Leu Val Phe Arg Thr Pro Leu Lys Gln 
             20                  25                  30 

aag ccc gtg cgc atc ctg tcg acc ggg ctg gcc ggt gag caa gag ttt      144 
Lys Pro Val Arg Ile Leu Ser Thr Gly Leu Ala Gly Glu Gln Glu Phe 
         35                  40                  45 

cac tcg atg ctt cgc gcg cga ttg acc cat acg ggt cag gtc gac atc      192 
His Ser Met Leu Arg Ala Arg Leu Thr His Thr Gly Gln Val Asp Ile 
     50                  55                  60 

gcg tcg gta ccg ctg gac gca gct att tgg gct tct ccc gct cga ctt      240 
Ala Ser Val Pro Leu Asp Ala Ala Ile Trp Ala Ser Pro Ala Arg Leu 
 65                  70                  75                  80 

gcc cag gca atg gat gcg ttg aat ggt acg cgt ctg atc gct ttt gtt      288 
Ala Gln Ala Met Asp Ala Leu Asn Gly Thr Arg Leu Ile Ala Phe Val 
                 85                  90                  95 

gag ccc agg aac gaa ttg ata ctg atg caa ttc ttg atg gat cgc ggg      336 
Glu Pro Arg Asn Glu Leu Ile Leu Met Gln Phe Leu Met Asp Arg Gly 
            100                 105                 110 

gct gcg gtg ctt att caa ggt gag cat gcg gtg gac agc aag ggg gtc      384 
Ala Ala Val Leu Ile Gln Gly Glu His Ala Val Asp Ser Lys Gly Val 
        115                 120                 125 

tct cgg cac gac ttt ctg agt acc cca tcc agt gcg gga att gga ggg      432 
Ser Arg His Asp Phe Leu Ser Thr Pro Ser Ser Ala Gly Ile Gly Gly 
    130                 135                 140 

gcg cta gcc gac agc ctg gca aaa ggg ggc tcg ccg ttc tct att tcc      480 
Ala Leu Ala Asp Ser Leu Ala Lys Gly Gly Ser Pro Phe Ser Ile Ser 
145                 150                 155                 160 

gtc cga gcg ctt ggc tcg gta act gct cag cca aga agt aat cag agt      528 
Val Arg Ala Leu Gly Ser Val Thr Ala Gln Pro Arg Ser Asn Gln Ser 
                165                 170                 175 

gag gtg gcc acc cac tgg acg acc gct ctg ggg acc tat tat gcc gat      576 
Glu Val Ala Thr His Trp Thr Thr Ala Leu Gly Thr Tyr Tyr Ala Asp 
            180                 185                 190 

atc gca gtg ggg cgc tgg gag ccg cag cgc gaa gtg gcc agc tat gga      624 
Ile Ala Val Gly Arg Trp Glu Pro Gln Arg Glu Val Ala Ser Tyr Gly 
        195                 200                 205 

agt gga cta atc atg gcg gaa cgg ctt gat cgt gtt gcc tca acc ttc      672 
Ser Gly Leu Ile Met Ala Glu Arg Leu Asp Arg Val Ala Ser Thr Phe 
    210                 215                 220 

att gca gat ctc tga                                                  687 
Ile Ala Asp Leu 
225 

 
           
             14  
             228  
             PRT  
             Pseudomonas sp.  
           
            14 

Met Thr Thr Arg Arg Asn Phe Leu Ile Gly Ala Ser Gln Val Gly Ala 
  1               5                  10                  15 

Leu Val Met Met Ser Pro Lys Leu Val Phe Arg Thr Pro Leu Lys Gln 
             20                  25                  30 

Lys Pro Val Arg Ile Leu Ser Thr Gly Leu Ala Gly Glu Gln Glu Phe 
         35                  40                  45 

His Ser Met Leu Arg Ala Arg Leu Thr His Thr Gly Gln Val Asp Ile 
     50                  55                  60 

Ala Ser Val Pro Leu Asp Ala Ala Ile Trp Ala Ser Pro Ala Arg Leu 
 65                  70                  75                  80 

Ala Gln Ala Met Asp Ala Leu Asn Gly Thr Arg Leu Ile Ala Phe Val 
                 85                  90                  95 

Glu Pro Arg Asn Glu Leu Ile Leu Met Gln Phe Leu Met Asp Arg Gly 
            100                 105                 110 

Ala Ala Val Leu Ile Gln Gly Glu His Ala Val Asp Ser Lys Gly Val 
        115                 120                 125 

Ser Arg His Asp Phe Leu Ser Thr Pro Ser Ser Ala Gly Ile Gly Gly 
    130                 135                 140 

Ala Leu Ala Asp Ser Leu Ala Lys Gly Gly Ser Pro Phe Ser Ile Ser 
145                 150                 155                 160 

Val Arg Ala Leu Gly Ser Val Thr Ala Gln Pro Arg Ser Asn Gln Ser 
                165                 170                 175 

Glu Val Ala Thr His Trp Thr Thr Ala Leu Gly Thr Tyr Tyr Ala Asp 
            180                 185                 190 

Ile Ala Val Gly Arg Trp Glu Pro Gln Arg Glu Val Ala Ser Tyr Gly 
        195                 200                 205 

Ser Gly Leu Ile Met Ala Glu Arg Leu Asp Arg Val Ala Ser Thr Phe 
    210                 215                 220 

Ile Ala Asp Leu 
225 

 
           
             15  
             1554  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1551)  
               product = “ Flavoprotein UE-Eugenol-Hydroxylase 
      ” /gene = “ehyB” 
             
           
            15 

atg gaa agc acc gta gtt ctt ccc gag ggt gtc acc ccg gag cag ttc       48 
Met Glu Ser Thr Val Val Leu Pro Glu Gly Val Thr Pro Glu Gln Phe 
  1               5                  10                  15 

acc aaa gcc atc agc gag ttc cgt cag gta ttg ggt gag gac agt gtt       96 
Thr Lys Ala Ile Ser Glu Phe Arg Gln Val Leu Gly Glu Asp Ser Val 
             20                  25                  30 

ctt gtc act gct gaa cga gtt gtt ccc tat acg aaa ctc ctc att cct      144 
Leu Val Thr Ala Glu Arg Val Val Pro Tyr Thr Lys Leu Leu Ile Pro 
         35                  40                  45 

aca cag gat gat gcc cag tac acc ccg gcc ggt gcc ttg act cct tct      192 
Thr Gln Asp Asp Ala Gln Tyr Thr Pro Ala Gly Ala Leu Thr Pro Ser 
     50                  55                  60 

tcg gtg gag cag gtc cag aaa gtc atg ggg atc tgc aat aag tac aag      240 
Ser Val Glu Gln Val Gln Lys Val Met Gly Ile Cys Asn Lys Tyr Lys 
 65                  70                  75                  80 

atc ccg gta tgg cca atc tct acc ggt cgg aac tgg ggg tat ggg tcc      288 
Ile Pro Val Trp Pro Ile Ser Thr Gly Arg Asn Trp Gly Tyr Gly Ser 
                 85                  90                  95 

gct tcg cct gca act cct ggg cag atg att ctt gac ctt cgc aag atg      336 
Ala Ser Pro Ala Thr Pro Gly Gln Met Ile Leu Asp Leu Arg Lys Met 
            100                 105                 110 

aac aag atc att gag atc gat gtt gag ggg tgt act gcc ctg ctc gag      384 
Asn Lys Ile Ile Glu Ile Asp Val Glu Gly Cys Thr Ala Leu Leu Glu 
        115                 120                 125 

ccg ggc gtt acc tac cag cag ctt cac gat tac atc aag gag cac aat      432 
Pro Gly Val Thr Tyr Gln Gln Leu His Asp Tyr Ile Lys Glu His Asn 
    130                 135                 140 

ctg ccc ttg atg ctg gat gtg ccg act att ggg cct atg gtt ggc ccg      480 
Leu Pro Leu Met Leu Asp Val Pro Thr Ile Gly Pro Met Val Gly Pro 
145                 150                 155                 160 

gtg ggt aac acg ctg gat cga ggc gtt ggt tat acg ccg tac ggc gag      528 
Val Gly Asn Thr Leu Asp Arg Gly Val Gly Tyr Thr Pro Tyr Gly Glu 
                165                 170                 175 

cac ttc atg atg cag tgt ggt atg gaa gtc gtc atg gcc gat ggc gaa      576 
His Phe Met Met Gln Cys Gly Met Glu Val Val Met Ala Asp Gly Glu 
            180                 185                 190 

atc ctc cgt act ggt atg ggc tcg gtg ccc aaa gcc aag act tgg cag      624 
Ile Leu Arg Thr Gly Met Gly Ser Val Pro Lys Ala Lys Thr Trp Gln 
        195                 200                 205 

gca ttc aaa tgg ggc tat ggt cca tat ctg gac ggt atc ttt acc cag      672 
Ala Phe Lys Trp Gly Tyr Gly Pro Tyr Leu Asp Gly Ile Phe Thr Gln 
    210                 215                 220 

tcc aac ttt ggt gtt gtg aca aag ctc ggg att tgg ttg atg ccc aag      720 
Ser Asn Phe Gly Val Val Thr Lys Leu Gly Ile Trp Leu Met Pro Lys 
225                 230                 235                 240 

ccg cca gtg atc aag tcg ttt atg atc cgt tat ccc aat gaa gct gat      768 
Pro Pro Val Ile Lys Ser Phe Met Ile Arg Tyr Pro Asn Glu Ala Asp 
                245                 250                 255 

gtg gtt aag gca att gat gct ttt cgc ccg ctg cgt att act cag ctg      816 
Val Val Lys Ala Ile Asp Ala Phe Arg Pro Leu Arg Ile Thr Gln Leu 
            260                 265                 270 

att cct aac gtc gtt ttg ttc atg cac ggc atg tac gaa acg gca atc      864 
Ile Pro Asn Val Val Leu Phe Met His Gly Met Tyr Glu Thr Ala Ile 
        275                 280                 285 

tgc cgg acg cgt gct gag gtt act tcg gac cca ggt cct att tct gaa      912 
Cys Arg Thr Arg Ala Glu Val Thr Ser Asp Pro Gly Pro Ile Ser Glu 
    290                 295                 300 

gcg gac gcc cgc aaa gca ttc aaa gag cta ggc gtt ggc tac tgg aac      960 
Ala Asp Ala Arg Lys Ala Phe Lys Glu Leu Gly Val Gly Tyr Trp Asn 
305                 310                 315                 320 

gtt tac ttc gcg ctt tac ggc aca gaa gag cag ata gcc gtc aat gaa     1008 
Val Tyr Phe Ala Leu Tyr Gly Thr Glu Glu Gln Ile Ala Val Asn Glu 
                325                 330                 335 

aag atc gtc cgc ggc atc ctc gaa ccg acg ggg ggt gag atc ctc acc     1056 
Lys Ile Val Arg Gly Ile Leu Glu Pro Thr Gly Gly Glu Ile Leu Thr 
            340                 345                 350 

gaa gag gag gct gga gat aac att ctt ttc cat cac cat aag cag ctc     1104 
Glu Glu Glu Ala Gly Asp Asn Ile Leu Phe His His His Lys Gln Leu 
        355                 360                 365 

atg aac ggc gag atg aca ttg gag gaa atg aat atc tac cag tgg cgc     1152 
Met Asn Gly Glu Met Thr Leu Glu Glu Met Asn Ile Tyr Gln Trp Arg 
    370                 375                 380 

gga gca ggt ggc ggt gct tgc tgg ttt gca ccg gtt gct cag gtc aag     1200 
Gly Ala Gly Gly Gly Ala Cys Trp Phe Ala Pro Val Ala Gln Val Lys 
385                 390                 395                 400 

ggg cat gag gca gag cag cag gtc aag ctt gct cag aag gtg ctt gca     1248 
Gly His Glu Ala Glu Gln Gln Val Lys Leu Ala Gln Lys Val Leu Ala 
                405                 410                 415 

aag cat ggg ttc gat tac acg gcg ggc ttt gcg att ggt tgg cgc gat     1296 
Lys His Gly Phe Asp Tyr Thr Ala Gly Phe Ala Ile Gly Trp Arg Asp 
            420                 425                 430 

ctt cac cat gtg atc gat gtg ctg tac gac cgt agc aat gcc gac gag     1344 
Leu His His Val Ile Asp Val Leu Tyr Asp Arg Ser Asn Ala Asp Glu 
        435                 440                 445 

aaa aag cgc gct tac gct tgc ttt gat gaa ttg atc gac gtc ttt gcg     1392 
Lys Lys Arg Ala Tyr Ala Cys Phe Asp Glu Leu Ile Asp Val Phe Ala 
    450                 455                 460 

gcc gaa ggc ttt gca agt tac agg acc aat att gcc ttt atg gac aaa     1440 
Ala Glu Gly Phe Ala Ser Tyr Arg Thr Asn Ile Ala Phe Met Asp Lys 
465                 470                 475                 480 

gtc gcc tct aag ttc ggc gct gag aat aag agg gtc aat cag aag atc     1488 
Val Ala Ser Lys Phe Gly Ala Glu Asn Lys Arg Val Asn Gln Lys Ile 
                485                 490                 495 

aag gct gcc ctt gat cca aac ggc atc atc gct ccc ggc aag tcg ggc     1536 
Lys Ala Ala Leu Asp Pro Asn Gly Ile Ile Ala Pro Gly Lys Ser Gly 
            500                 505                 510 

att cat ctt ccc aaa taa                                             1554 
Ile His Leu Pro Lys 
        515 

 
           
             16  
             517  
             PRT  
             Pseudomonas sp.  
           
            16 

Met Glu Ser Thr Val Val Leu Pro Glu Gly Val Thr Pro Glu Gln Phe 
  1               5                  10                  15 

Thr Lys Ala Ile Ser Glu Phe Arg Gln Val Leu Gly Glu Asp Ser Val 
             20                  25                  30 

Leu Val Thr Ala Glu Arg Val Val Pro Tyr Thr Lys Leu Leu Ile Pro 
         35                  40                  45 

Thr Gln Asp Asp Ala Gln Tyr Thr Pro Ala Gly Ala Leu Thr Pro Ser 
     50                  55                  60 

Ser Val Glu Gln Val Gln Lys Val Met Gly Ile Cys Asn Lys Tyr Lys 
 65                  70                  75                  80 

Ile Pro Val Trp Pro Ile Ser Thr Gly Arg Asn Trp Gly Tyr Gly Ser 
                 85                  90                  95 

Ala Ser Pro Ala Thr Pro Gly Gln Met Ile Leu Asp Leu Arg Lys Met 
            100                 105                 110 

Asn Lys Ile Ile Glu Ile Asp Val Glu Gly Cys Thr Ala Leu Leu Glu 
        115                 120                 125 

Pro Gly Val Thr Tyr Gln Gln Leu His Asp Tyr Ile Lys Glu His Asn 
    130                 135                 140 

Leu Pro Leu Met Leu Asp Val Pro Thr Ile Gly Pro Met Val Gly Pro 
145                 150                 155                 160 

Val Gly Asn Thr Leu Asp Arg Gly Val Gly Tyr Thr Pro Tyr Gly Glu 
                165                 170                 175 

His Phe Met Met Gln Cys Gly Met Glu Val Val Met Ala Asp Gly Glu 
            180                 185                 190 

Ile Leu Arg Thr Gly Met Gly Ser Val Pro Lys Ala Lys Thr Trp Gln 
        195                 200                 205 

Ala Phe Lys Trp Gly Tyr Gly Pro Tyr Leu Asp Gly Ile Phe Thr Gln 
    210                 215                 220 

Ser Asn Phe Gly Val Val Thr Lys Leu Gly Ile Trp Leu Met Pro Lys 
225                 230                 235                 240 

Pro Pro Val Ile Lys Ser Phe Met Ile Arg Tyr Pro Asn Glu Ala Asp 
                245                 250                 255 

Val Val Lys Ala Ile Asp Ala Phe Arg Pro Leu Arg Ile Thr Gln Leu 
            260                 265                 270 

Ile Pro Asn Val Val Leu Phe Met His Gly Met Tyr Glu Thr Ala Ile 
        275                 280                 285 

Cys Arg Thr Arg Ala Glu Val Thr Ser Asp Pro Gly Pro Ile Ser Glu 
    290                 295                 300 

Ala Asp Ala Arg Lys Ala Phe Lys Glu Leu Gly Val Gly Tyr Trp Asn 
305                 310                 315                 320 

Val Tyr Phe Ala Leu Tyr Gly Thr Glu Glu Gln Ile Ala Val Asn Glu 
                325                 330                 335 

Lys Ile Val Arg Gly Ile Leu Glu Pro Thr Gly Gly Glu Ile Leu Thr 
            340                 345                 350 

Glu Glu Glu Ala Gly Asp Asn Ile Leu Phe His His His Lys Gln Leu 
        355                 360                 365 

Met Asn Gly Glu Met Thr Leu Glu Glu Met Asn Ile Tyr Gln Trp Arg 
    370                 375                 380 

Gly Ala Gly Gly Gly Ala Cys Trp Phe Ala Pro Val Ala Gln Val Lys 
385                 390                 395                 400 

Gly His Glu Ala Glu Gln Gln Val Lys Leu Ala Gln Lys Val Leu Ala 
                405                 410                 415 

Lys His Gly Phe Asp Tyr Thr Ala Gly Phe Ala Ile Gly Trp Arg Asp 
            420                 425                 430 

Leu His His Val Ile Asp Val Leu Tyr Asp Arg Ser Asn Ala Asp Glu 
        435                 440                 445 

Lys Lys Arg Ala Tyr Ala Cys Phe Asp Glu Leu Ile Asp Val Phe Ala 
    450                 455                 460 

Ala Glu Gly Phe Ala Ser Tyr Arg Thr Asn Ile Ala Phe Met Asp Lys 
465                 470                 475                 480 

Val Ala Ser Lys Phe Gly Ala Glu Asn Lys Arg Val Asn Gln Lys Ile 
                485                 490                 495 

Lys Ala Ala Leu Asp Pro Asn Gly Ile Ile Ala Pro Gly Lys Ser Gly 
            500                 505                 510 

Ile His Leu Pro Lys 
        515 

 
           
             17  
             861  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(858)  
               gene = “ORF2” 
             
           
            17 

atg att gca atc act gcg ggc acc gga agt ctt ggt cgg gct atc gtt       48 
Met Ile Ala Ile Thr Ala Gly Thr Gly Ser Leu Gly Arg Ala Ile Val 
  1               5                  10                  15 

gag cga cta ggg gac tgc ggt ctt atc ggt caa gtt cga ttg acg gct       96 
Glu Arg Leu Gly Asp Cys Gly Leu Ile Gly Gln Val Arg Leu Thr Ala 
             20                  25                  30 

cgc gat cct aaa agg ctt cgt gcc gct gcc gag gaa ggg ttt cag gtc      144 
Arg Asp Pro Lys Arg Leu Arg Ala Ala Ala Glu Glu Gly Phe Gln Val 
         35                  40                  45 

gct aag gcg gat tac gcc gat att ggg agt ctt gac cag gca tta cag      192 
Ala Lys Ala Asp Tyr Ala Asp Ile Gly Ser Leu Asp Gln Ala Leu Gln 
     50                  55                  60 

ggg gta gac gta tta ctc ctg att tct ggt act gca ccc aat gaa ata      240 
Gly Val Asp Val Leu Leu Leu Ile Ser Gly Thr Ala Pro Asn Glu Ile 
 65                  70                  75                  80 

agg atc caa cag cat aag tcg gtc atc gac gcg gca aaa cga aac ggc      288 
Arg Ile Gln Gln His Lys Ser Val Ile Asp Ala Ala Lys Arg Asn Gly 
                 85                  90                  95 

gtg tcg cgt att gtg tat acc agc ttc ata aat cca agt act cgc agc      336 
Val Ser Arg Ile Val Tyr Thr Ser Phe Ile Asn Pro Ser Thr Arg Ser 
            100                 105                 110 

agg tct att tgg gcc tcc att cat cgt gaa act gag act tac ctc agg      384 
Arg Ser Ile Trp Ala Ser Ile His Arg Glu Thr Glu Thr Tyr Leu Arg 
        115                 120                 125 

cag tct ggg gtg aag ttt acg att gtc cga aat aat cag tat gcg tct      432 
Gln Ser Gly Val Lys Phe Thr Ile Val Arg Asn Asn Gln Tyr Ala Ser 
    130                 135                 140 

aac ctg gat ctg ttg ctg ctg agg gct caa gac agc gga ata ttt gcc      480 
Asn Leu Asp Leu Leu Leu Leu Arg Ala Gln Asp Ser Gly Ile Phe Ala 
145                 150                 155                 160 

att ccc ggg gcg aag ggg cgg gtg gcg tac gtc tct cat cgc gac gtt      528 
Ile Pro Gly Ala Lys Gly Arg Val Ala Tyr Val Ser His Arg Asp Val 
                165                 170                 175 

gcc gct gcc atc tgt agt gtc ctg acg acc gcc gga cac gat aac agg      576 
Ala Ala Ala Ile Cys Ser Val Leu Thr Thr Ala Gly His Asp Asn Arg 
            180                 185                 190 

atc tac cag ctc aca ggc tct gag gct ctc aat ggg ctc gag atc gcg      624 
Ile Tyr Gln Leu Thr Gly Ser Glu Ala Leu Asn Gly Leu Glu Ile Ala 
        195                 200                 205 

gag att ctt ggt ggg gtg ctc ggg cgt cca gtg cgc gcg atg gat gcc      672 
Glu Ile Leu Gly Gly Val Leu Gly Arg Pro Val Arg Ala Met Asp Ala 
    210                 215                 220 

tcg cct gac gag ttt gct gcc agc ttt cgc gag gct gga ttc cct gag      720 
Ser Pro Asp Glu Phe Ala Ala Ser Phe Arg Glu Ala Gly Phe Pro Glu 
225                 230                 235                 240 

ttt atg gtt gaa ggc cta cta agc att tat gcc gct tca ggt gct ggg      768 
Phe Met Val Glu Gly Leu Leu Ser Ile Tyr Ala Ala Ser Gly Ala Gly 
                245                 250                 255 

gag tac caa tcc gtc agt cct gat gtt ggg ttg ttg acg gga cga cgt      816 
Glu Tyr Gln Ser Val Ser Pro Asp Val Gly Leu Leu Thr Gly Arg Arg 
            260                 265                 270 

gcc gaa tcg atg cga act tac ata cag cgt cta gtt tgg cct tga          861 
Ala Glu Ser Met Arg Thr Tyr Ile Gln Arg Leu Val Trp Pro 
        275                 280                 285 

 
           
             18  
             286  
             PRT  
             Pseudomonas sp.  
           
            18 

Met Ile Ala Ile Thr Ala Gly Thr Gly Ser Leu Gly Arg Ala Ile Val 
  1               5                  10                  15 

Glu Arg Leu Gly Asp Cys Gly Leu Ile Gly Gln Val Arg Leu Thr Ala 
             20                  25                  30 

Arg Asp Pro Lys Arg Leu Arg Ala Ala Ala Glu Glu Gly Phe Gln Val 
         35                  40                  45 

Ala Lys Ala Asp Tyr Ala Asp Ile Gly Ser Leu Asp Gln Ala Leu Gln 
     50                  55                  60 

Gly Val Asp Val Leu Leu Leu Ile Ser Gly Thr Ala Pro Asn Glu Ile 
 65                  70                  75                  80 

Arg Ile Gln Gln His Lys Ser Val Ile Asp Ala Ala Lys Arg Asn Gly 
                 85                  90                  95 

Val Ser Arg Ile Val Tyr Thr Ser Phe Ile Asn Pro Ser Thr Arg Ser 
            100                 105                 110 

Arg Ser Ile Trp Ala Ser Ile His Arg Glu Thr Glu Thr Tyr Leu Arg 
        115                 120                 125 

Gln Ser Gly Val Lys Phe Thr Ile Val Arg Asn Asn Gln Tyr Ala Ser 
    130                 135                 140 

Asn Leu Asp Leu Leu Leu Leu Arg Ala Gln Asp Ser Gly Ile Phe Ala 
145                 150                 155                 160 

Ile Pro Gly Ala Lys Gly Arg Val Ala Tyr Val Ser His Arg Asp Val 
                165                 170                 175 

Ala Ala Ala Ile Cys Ser Val Leu Thr Thr Ala Gly His Asp Asn Arg 
            180                 185                 190 

Ile Tyr Gln Leu Thr Gly Ser Glu Ala Leu Asn Gly Leu Glu Ile Ala 
        195                 200                 205 

Glu Ile Leu Gly Gly Val Leu Gly Arg Pro Val Arg Ala Met Asp Ala 
    210                 215                 220 

Ser Pro Asp Glu Phe Ala Ala Ser Phe Arg Glu Ala Gly Phe Pro Glu 
225                 230                 235                 240 

Phe Met Val Glu Gly Leu Leu Ser Ile Tyr Ala Ala Ser Gly Ala Gly 
                245                 250                 255 

Glu Tyr Gln Ser Val Ser Pro Asp Val Gly Leu Leu Thr Gly Arg Arg 
            260                 265                 270 

Ala Glu Ser Met Arg Thr Tyr Ile Gln Arg Leu Val Trp Pro 
        275                 280                 285 

 
           
             19  
             1011  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1008)  
               product = “Alkohol-Dehydrogenase” / gene =
      “adh” 
             
           
            19 

atg aag gct tat gag ctt cac aag att tcg gaa cag gta gag gtc agg       48 
Met Lys Ala Tyr Glu Leu His Lys Ile Ser Glu Gln Val Glu Val Arg 
  1               5                  10                  15 

ctc cag cca act cgg ccc cgc ccg cag ttg aat cat ggc gag gtc ctc       96 
Leu Gln Pro Thr Arg Pro Arg Pro Gln Leu Asn His Gly Glu Val Leu 
             20                  25                  30 

atc agg gtc cat gca gcc tcg ctc aac ttt cgc gat ttg atg atc ttg      144 
Ile Arg Val His Ala Ala Ser Leu Asn Phe Arg Asp Leu Met Ile Leu 
         35                  40                  45 

gcc ggt cgc tat ccg ggt caa atg aaa ccc gat gtg atc ccg ctg tcc      192 
Ala Gly Arg Tyr Pro Gly Gln Met Lys Pro Asp Val Ile Pro Leu Ser 
     50                  55                  60 

gat ggt gct ggc gag att gtg gag gtc ggg cct ggc gta tct tcg gag      240 
Asp Gly Ala Gly Glu Ile Val Glu Val Gly Pro Gly Val Ser Ser Glu 
 65                  70                  75                  80 

gtg cag ggt cag cgc gta gcc agc acc ttt ttc cct aac tgg cgg gcc      288 
Val Gln Gly Gln Arg Val Ala Ser Thr Phe Phe Pro Asn Trp Arg Ala 
                 85                  90                  95 

gga aag att acc gag ccg gct att gag gtg tcg ttg ggc ttc ggt atg      336 
Gly Lys Ile Thr Glu Pro Ala Ile Glu Val Ser Leu Gly Phe Gly Met 
            100                 105                 110 

gac ggg atg ctc gcg gaa tac gtt gct ctg ccc tat gag gca acg ata      384 
Asp Gly Met Leu Ala Glu Tyr Val Ala Leu Pro Tyr Glu Ala Thr Ile 
        115                 120                 125 

ccg ata ccg gag cac ctg tcg tac gag gag gct gca aca ttg cct tgc      432 
Pro Ile Pro Glu His Leu Ser Tyr Glu Glu Ala Ala Thr Leu Pro Cys 
    130                 135                 140 

gcg gcg cta acc gct tgg aat gcg ttg acc gaa gtg ggg cgt gtc aag      480 
Ala Ala Leu Thr Ala Trp Asn Ala Leu Thr Glu Val Gly Arg Val Lys 
145                 150                 155                 160 

gcc ggt gat acg gtc ttg ttg ctt ggc act ggc ggt gtc tcg atg ttc      528 
Ala Gly Asp Thr Val Leu Leu Leu Gly Thr Gly Gly Val Ser Met Phe 
                165                 170                 175 

gcg ttg cag ttc gcc aag ctc ttg ggg gcg acg gtc att cac acc tcg      576 
Ala Leu Gln Phe Ala Lys Leu Leu Gly Ala Thr Val Ile His Thr Ser 
            180                 185                 190 

agc agt gaa caa aag ctg gag agg gtg aaa gcg atg ggg gct gat cat      624 
Ser Ser Glu Gln Lys Leu Glu Arg Val Lys Ala Met Gly Ala Asp His 
        195                 200                 205 

ctg atc aac tac cgc aat tcg cca ggg tgg gac cgt act gtc ctg gat      672 
Leu Ile Asn Tyr Arg Asn Ser Pro Gly Trp Asp Arg Thr Val Leu Asp 
    210                 215                 220 

ctc acc gcg ggg cga ggg gtt gac ctg gta gtc gag gta ggg ggg gcg      720 
Leu Thr Ala Gly Arg Gly Val Asp Leu Val Val Glu Val Gly Gly Ala 
225                 230                 235                 240 

ggg acc ttg gag cgc tca ctt cgt gcg gtc aag gta ggc ggt att gtc      768 
Gly Thr Leu Glu Arg Ser Leu Arg Ala Val Lys Val Gly Gly Ile Val 
                245                 250                 255 

gcc acg att ggg cta gtg gct ggc gtt ggc ccg att gac cca ttg ccg      816 
Ala Thr Ile Gly Leu Val Ala Gly Val Gly Pro Ile Asp Pro Leu Pro 
            260                 265                 270 

ctt atc tcc agg gct att cag ctc tcg ggc gtc tat gtc ggt tcc cgg      864 
Leu Ile Ser Arg Ala Ile Gln Leu Ser Gly Val Tyr Val Gly Ser Arg 
        275                 280                 285 

gaa atg ttt ctc tca atg aac aaa gcc att gca tca gcc gaa atc aag      912 
Glu Met Phe Leu Ser Met Asn Lys Ala Ile Ala Ser Ala Glu Ile Lys 
    290                 295                 300 

cca gtg atc gat tgc tgc ttc ccc atc gac gag gtt gga gat gct tat      960 
Pro Val Ile Asp Cys Cys Phe Pro Ile Asp Glu Val Gly Asp Ala Tyr 
305                 310                 315                 320 

gag tac atg cgt agc ggc aat cac ctt ggc aaa gta gtt atc acg atc     1008 
Glu Tyr Met Arg Ser Gly Asn His Leu Gly Lys Val Val Ile Thr Ile 
                325                 330                 335 

taa                                                                 1011 

 
           
             20  
             336  
             PRT  
             Pseudomonas sp.  
           
            20 

Met Lys Ala Tyr Glu Leu His Lys Ile Ser Glu Gln Val Glu Val Arg 
  1               5                  10                  15 

Leu Gln Pro Thr Arg Pro Arg Pro Gln Leu Asn His Gly Glu Val Leu 
             20                  25                  30 

Ile Arg Val His Ala Ala Ser Leu Asn Phe Arg Asp Leu Met Ile Leu 
         35                  40                  45 

Ala Gly Arg Tyr Pro Gly Gln Met Lys Pro Asp Val Ile Pro Leu Ser 
     50                  55                  60 

Asp Gly Ala Gly Glu Ile Val Glu Val Gly Pro Gly Val Ser Ser Glu 
 65                  70                  75                  80 

Val Gln Gly Gln Arg Val Ala Ser Thr Phe Phe Pro Asn Trp Arg Ala 
                 85                  90                  95 

Gly Lys Ile Thr Glu Pro Ala Ile Glu Val Ser Leu Gly Phe Gly Met 
            100                 105                 110 

Asp Gly Met Leu Ala Glu Tyr Val Ala Leu Pro Tyr Glu Ala Thr Ile 
        115                 120                 125 

Pro Ile Pro Glu His Leu Ser Tyr Glu Glu Ala Ala Thr Leu Pro Cys 
    130                 135                 140 

Ala Ala Leu Thr Ala Trp Asn Ala Leu Thr Glu Val Gly Arg Val Lys 
145                 150                 155                 160 

Ala Gly Asp Thr Val Leu Leu Leu Gly Thr Gly Gly Val Ser Met Phe 
                165                 170                 175 

Ala Leu Gln Phe Ala Lys Leu Leu Gly Ala Thr Val Ile His Thr Ser 
            180                 185                 190 

Ser Ser Glu Gln Lys Leu Glu Arg Val Lys Ala Met Gly Ala Asp His 
        195                 200                 205 

Leu Ile Asn Tyr Arg Asn Ser Pro Gly Trp Asp Arg Thr Val Leu Asp 
    210                 215                 220 

Leu Thr Ala Gly Arg Gly Val Asp Leu Val Val Glu Val Gly Gly Ala 
225                 230                 235                 240 

Gly Thr Leu Glu Arg Ser Leu Arg Ala Val Lys Val Gly Gly Ile Val 
                245                 250                 255 

Ala Thr Ile Gly Leu Val Ala Gly Val Gly Pro Ile Asp Pro Leu Pro 
            260                 265                 270 

Leu Ile Ser Arg Ala Ile Gln Leu Ser Gly Val Tyr Val Gly Ser Arg 
        275                 280                 285 

Glu Met Phe Leu Ser Met Asn Lys Ala Ile Ala Ser Ala Glu Ile Lys 
    290                 295                 300 

Pro Val Ile Asp Cys Cys Phe Pro Ile Asp Glu Val Gly Asp Ala Tyr 
305                 310                 315                 320 

Glu Tyr Met Arg Ser Gly Asn His Leu Gly Lys Val Val Ile Thr Ile 
                325                 330                 335 

 
           
             21  
             1518  
             DNA  
             Pseudomonas sp.  
           
            21 

tcaccgtcgt gatcgggatt ggaaattcgt gcgaggacag cggccacgta ccggcgccct     60 

gaagggctgg aaggttggag tttcgttaag gtctggtacc cagcagccat ggagagcggc    120 

ccttagccgg aatggcagct tgatggttgc cacgggacca gactggatgt cttgagtgtc    180 

gagaattacc agatcgctgc gattttcatc gaggcgacca accacggtca gcaagtaccc    240 

gtcaccttcg gcggcggtcg gacttctagg gacgaaggcc ggctcctggg ccgccgaggc    300 

ttcgccggag taccagaggt cgtagtcacc tcggtggttg tcccagatgc cgagtgagtt    360 

gtacgcgaat atcttctcgg cctgctgatg cgcaagtggt ttgcgtggat cgtccacccc    420 

cataaagcca tagcggttgc attgcagggc gaacgaagaa tccatgattg gcatttccgc    480 

aaagaaatcg tgtagccggg ttcgcttgat ctcgtcgctg ctgctatcga ggtcaatttc    540 

ccaacgagtc aggcgtggta cggctttctc aggggcgaag ggttggtttt gtgagttggg    600 

gaaggggaac ggcaggattt cactttccat aaggtcgata taaatcttgg ttccgacttc    660 

ccaagcattc acaacatgaa atacccagag cgccggtgcc ttgagccagc gaatcagact    720 

gccctggcgc ggcgcgagta cgccaatgta gctgcccagt tccggctccc acatataaat    780 

tggctgtttc gccttgaggc gggacaggct gttggtggcc ggcataattg ggaaaatgga    840 

ccaatttcgg gtaatggcaa agtcgtgcat gaatgcgcca tagggctgct caaaccaagt    900 

ttcatgtgtc accttgccgt gcttgtcgac aatgtaatag gccatgtctg gagttgcttc    960 

gcccttagct gccgaaccga agaacaacaa gtcacccgtt tccgggtcat attttggatg   1020 

ggcggtgtgg gtttggctgg taacttggcc gtcgtagtcg aagtgtccgc gagtttcaag   1080 

tgtacgagga tccagttcgt acggtaggcc gtcttccttc accgccagca ccttgccgtg   1140 

atggctaatg atgcttgtat tggcaacggt gcggtctagt ccttttacac tggtgtcgtc   1200 

ggtatagggg tttctgtaca tgccaaatag cgattttcgc gctagtcgtt cggccgtgaa   1260 

tcgagcggtt ttaacccagc gactgatgaa gtcgacatga ccatcttcga agtggaaggc   1320 

agaggccatt ccatctccat ctatgaaggt gtggaatttt tgtggggtaa cttgaggctc   1380 

tggcgtatta cggtagaacg ttccatttat tgattttggg atttcgccgt caacctctag   1440 

atcgaacaag tctgcctcta tacgggtggg gagaagtgtt cctactaatt gcgggtcgtt   1500 

gcggttgaat ctcgccat                                                 1518 

 
           
             22  
             505  
             PRT  
             Pseudomonas sp.  
           
            22 

Met Ala Arg Phe Asn Arg Asn Asp Pro Gln Leu Val Gly Thr Leu Leu 
  1               5                  10                  15 

Pro Thr Arg Ile Glu Ala Asp Leu Phe Asp Leu Glu Val Asp Gly Glu 
             20                  25                  30 

Ile Pro Lys Ser Ile Asn Gly Thr Phe Tyr Arg Asn Thr Pro Glu Pro 
         35                  40                  45 

Gln Val Thr Pro Gln Lys Phe His Thr Phe Ile Asp Gly Asp Gly Met 
     50                  55                  60 

Ala Ser Ala Phe His Phe Glu Asp Gly His Val Asp Phe Ile Ser Arg 
 65                  70                  75                  80 

Trp Val Lys Thr Ala Arg Phe Thr Ala Glu Arg Leu Ala Arg Lys Ser 
                 85                  90                  95 

Leu Phe Gly Met Tyr Arg Asn Pro Tyr Thr Asp Asp Thr Ser Val Lys 
            100                 105                 110 

Gly Leu Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Ser His His Gly 
        115                 120                 125 

Lys Val Leu Ala Val Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro 
    130                 135                 140 

Arg Thr Leu Glu Thr Arg Gly His Phe Asp Tyr Asp Gly Gln Val Thr 
145                 150                 155                 160 

Ser Gln Thr His Thr Ala His Pro Lys Tyr Asp Pro Glu Thr Gly Asp 
                165                 170                 175 

Leu Leu Phe Phe Gly Ser Ala Ala Lys Gly Glu Ala Thr Pro Asp Met 
            180                 185                 190 

Ala Tyr Tyr Ile Val Asp Lys His Gly Lys Val Thr His Glu Thr Trp 
        195                 200                 205 

Phe Glu Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Ile Thr Arg 
    210                 215                 220 

Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Ser Arg 
225                 230                 235                 240 

Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Leu Gly Ser 
                245                 250                 255 

Tyr Ile Gly Val Leu Ala Pro Arg Gln Gly Ser Leu Ile Arg Trp Leu 
            260                 265                 270 

Lys Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu Val 
        275                 280                 285 

Gly Thr Lys Ile Tyr Ile Asp Leu Met Glu Ser Glu Ile Leu Pro Phe 
    290                 295                 300 

Pro Phe Pro Asn Ser Gln Asn Gln Pro Phe Ala Pro Glu Lys Ala Val 
305                 310                 315                 320 

Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asp Ser Ser Ser Asp Glu 
                325                 330                 335 

Ile Lys Arg Thr Arg Leu His Asp Phe Phe Ala Glu Met Pro Ile Met 
            340                 345                 350 

Asp Ser Ser Phe Ala Leu Gln Cys Asn Arg Tyr Gly Phe Met Gly Val 
        355                 360                 365 

Asp Asp Pro Arg Lys Pro Leu Ala His Gln Gln Ala Glu Lys Ile Phe 
    370                 375                 380 

Ala Tyr Asn Ser Leu Gly Ile Trp Asp Asn His Arg Gly Asp Tyr Asp 
385                 390                 395                 400 

Leu Trp Tyr Ser Gly Glu Ala Ser Ala Ala Gln Glu Pro Ala Phe Val 
                405                 410                 415 

Pro Arg Ser Pro Thr Ala Ala Glu Gly Asp Gly Tyr Leu Leu Thr Val 
            420                 425                 430 

Val Gly Arg Leu Asp Glu Asn Arg Ser Asp Leu Val Ile Leu Asp Thr 
        435                 440                 445 

Gln Asp Ile Gln Ser Gly Pro Val Ala Thr Ile Lys Leu Pro Phe Arg 
    450                 455                 460 

Leu Arg Ala Ala Leu His Gly Cys Trp Val Pro Asp Leu Asn Glu Thr 
465                 470                 475                 480 

Pro Thr Phe Gln Pro Phe Arg Ala Pro Val Arg Gly Arg Cys Pro Arg 
                485                 490                 495 

Thr Asn Phe Gln Ser Arg Ser Arg Arg 
            500                 505 

 
           
             23  
             951  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(948)  
               gene = “ORF3” 
             
           
            23 

atg aca act att cgg tgg cgg cgt atg tcc att cac tct gag ggg atc       48 
Met Thr Thr Ile Arg Trp Arg Arg Met Ser Ile His Ser Glu Gly Ile 
  1               5                  10                  15 

act ctc gcg gat tcg ccg ctg cat tgg gcg cat acc ctg aat gga tca       96 
Thr Leu Ala Asp Ser Pro Leu His Trp Ala His Thr Leu Asn Gly Ser 
             20                  25                  30 

atg cgt act cat ttc gaa gtc cag cgt ctt gag cgg ggt aga ggt gcc      144 
Met Arg Thr His Phe Glu Val Gln Arg Leu Glu Arg Gly Arg Gly Ala 
         35                  40                  45 

tcc ctt gcc cga tct aga ttt ggc gcg ggt gag ctg tac agt gcc att      192 
Ser Leu Ala Arg Ser Arg Phe Gly Ala Gly Glu Leu Tyr Ser Ala Ile 
     50                  55                  60 

gca cca agc cag gta ctt cgc cac ttc aac gac cag cga aat gct gat      240 
Ala Pro Ser Gln Val Leu Arg His Phe Asn Asp Gln Arg Asn Ala Asp 
 65                  70                  75                  80 

gag gct gag cac agc tat ttg att cag ata cga agt ggc gct ttg ggc      288 
Glu Ala Glu His Ser Tyr Leu Ile Gln Ile Arg Ser Gly Ala Leu Gly 
                 85                  90                  95 

gtt gca tcc ggc gga aga aag gtg atc ttg gca aat ggt gat tgc tcc      336 
Val Ala Ser Gly Gly Arg Lys Val Ile Leu Ala Asn Gly Asp Cys Ser 
            100                 105                 110 

ata gtt gat agt cgc caa gac ttc aca ctt tcc tcg aac tct tcg acc      384 
Ile Val Asp Ser Arg Gln Asp Phe Thr Leu Ser Ser Asn Ser Ser Thr 
        115                 120                 125 

caa ggt gtc gta ata cgc ttt ccg gtg agt tgg ctg gga gcg tgg gtg      432 
Gln Gly Val Val Ile Arg Phe Pro Val Ser Trp Leu Gly Ala Trp Val 
    130                 135                 140 

tcc aat ccg gag gat ctt atc gcc cga cga gtt gat gct gag gta ggg      480 
Ser Asn Pro Glu Asp Leu Ile Ala Arg Arg Val Asp Ala Glu Val Gly 
145                 150                 155                 160 

tgg ggt agg gcg cta agc gca tcg gtt tct aat cta gat cca ttg cgc      528 
Trp Gly Arg Ala Leu Ser Ala Ser Val Ser Asn Leu Asp Pro Leu Arg 
                165                 170                 175 

atc gac gat tta ggt agc aat gta aat ggc att gca gag cat gtt gct      576 
Ile Asp Asp Leu Gly Ser Asn Val Asn Gly Ile Ala Glu His Val Ala 
            180                 185                 190 

atg tta att tca cta gca agt tct gcg gtt agt tct gaa gat ggg ggt      624 
Met Leu Ile Ser Leu Ala Ser Ser Ala Val Ser Ser Glu Asp Gly Gly 
        195                 200                 205 

gtg gct ctt cgg aaa atg agg gaa gtg aag aga gta ctc gag cag agt      672 
Val Ala Leu Arg Lys Met Arg Glu Val Lys Arg Val Leu Glu Gln Ser 
    210                 215                 220 

ttc gca gac gct aat ctc ggg ccg gaa agt gtt tca agt caa tta gga      720 
Phe Ala Asp Ala Asn Leu Gly Pro Glu Ser Val Ser Ser Gln Leu Gly 
225                 230                 235                 240 

att tcg aaa cgc tat ttg cat tat gtc ttt gct gcg tgc ggt acg acc      768 
Ile Ser Lys Arg Tyr Leu His Tyr Val Phe Ala Ala Cys Gly Thr Thr 
                245                 250                 255 

ttt ggt cgc gag ctg ttg gaa ata cgc ctg ggc aaa gct tat cga atg      816 
Phe Gly Arg Glu Leu Leu Glu Ile Arg Leu Gly Lys Ala Tyr Arg Met 
            260                 265                 270 

ctc tgt gcg gcg agt gac tcg ggt gct gtg ctg aag gtg gcc atg tcc      864 
Leu Cys Ala Ala Ser Asp Ser Gly Ala Val Leu Lys Val Ala Met Ser 
        275                 280                 285 

tca ggt ttt tcg gat tca agc cat ttc agc aag aaa ttt aag gaa aga      912 
Ser Gly Phe Ser Asp Ser Ser His Phe Ser Lys Lys Phe Lys Glu Arg 
    290                 295                 300 

tac ggt gtt tcg cct gtc tcc ttg gtg agg cag gct tga                  951 
Tyr Gly Val Ser Pro Val Ser Leu Val Arg Gln Ala 
305                 310                 315 

 
           
             24  
             316  
             PRT  
             Pseudomonas sp.  
           
            24 

Met Thr Thr Ile Arg Trp Arg Arg Met Ser Ile His Ser Glu Gly Ile 
  1               5                  10                  15 

Thr Leu Ala Asp Ser Pro Leu His Trp Ala His Thr Leu Asn Gly Ser 
             20                  25                  30 

Met Arg Thr His Phe Glu Val Gln Arg Leu Glu Arg Gly Arg Gly Ala 
         35                  40                  45 

Ser Leu Ala Arg Ser Arg Phe Gly Ala Gly Glu Leu Tyr Ser Ala Ile 
     50                  55                  60 

Ala Pro Ser Gln Val Leu Arg His Phe Asn Asp Gln Arg Asn Ala Asp 
 65                  70                  75                  80 

Glu Ala Glu His Ser Tyr Leu Ile Gln Ile Arg Ser Gly Ala Leu Gly 
                 85                  90                  95 

Val Ala Ser Gly Gly Arg Lys Val Ile Leu Ala Asn Gly Asp Cys Ser 
            100                 105                 110 

Ile Val Asp Ser Arg Gln Asp Phe Thr Leu Ser Ser Asn Ser Ser Thr 
        115                 120                 125 

Gln Gly Val Val Ile Arg Phe Pro Val Ser Trp Leu Gly Ala Trp Val 
    130                 135                 140 

Ser Asn Pro Glu Asp Leu Ile Ala Arg Arg Val Asp Ala Glu Val Gly 
145                 150                 155                 160 

Trp Gly Arg Ala Leu Ser Ala Ser Val Ser Asn Leu Asp Pro Leu Arg 
                165                 170                 175 

Ile Asp Asp Leu Gly Ser Asn Val Asn Gly Ile Ala Glu His Val Ala 
            180                 185                 190 

Met Leu Ile Ser Leu Ala Ser Ser Ala Val Ser Ser Glu Asp Gly Gly 
        195                 200                 205 

Val Ala Leu Arg Lys Met Arg Glu Val Lys Arg Val Leu Glu Gln Ser 
    210                 215                 220 

Phe Ala Asp Ala Asn Leu Gly Pro Glu Ser Val Ser Ser Gln Leu Gly 
225                 230                 235                 240 

Ile Ser Lys Arg Tyr Leu His Tyr Val Phe Ala Ala Cys Gly Thr Thr 
                245                 250                 255 

Phe Gly Arg Glu Leu Leu Glu Ile Arg Leu Gly Lys Ala Tyr Arg Met 
            260                 265                 270 

Leu Cys Ala Ala Ser Asp Ser Gly Ala Val Leu Lys Val Ala Met Ser 
        275                 280                 285 

Ser Gly Phe Ser Asp Ser Ser His Phe Ser Lys Lys Phe Lys Glu Arg 
    290                 295                 300 

Tyr Gly Val Ser Pro Val Ser Leu Val Arg Gln Ala 
305                 310                 315 

 
           
             25  
             735  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(732)  
               product = “Enoyl-CoA-Hydratase” / gene = “ech” 
             
           
            25 

atg agc cca act ctc aat cga gag atg gtc gag gtt ctg gag gtg ctg       48 
Met Ser Pro Thr Leu Asn Arg Glu Met Val Glu Val Leu Glu Val Leu 
  1               5                  10                  15 

gag cag gac gca gat gct cgc gtg ctt gtt ctg act ggt gca ggc gaa       96 
Glu Gln Asp Ala Asp Ala Arg Val Leu Val Leu Thr Gly Ala Gly Glu 
             20                  25                  30 

tcc tgg acc gcg ggc atg gac ctg aag gag tat ttc cgc gag acc gat      144 
Ser Trp Thr Ala Gly Met Asp Leu Lys Glu Tyr Phe Arg Glu Thr Asp 
         35                  40                  45 

gct ggc ccc gaa att ctg caa gag aag att cgt cgc gaa gcg tcg acc      192 
Ala Gly Pro Glu Ile Leu Gln Glu Lys Ile Arg Arg Glu Ala Ser Thr 
     50                  55                  60 

tgg cag tgg aag ctc ctg cgg atg tac acc aag ccg acc atc gcg atg      240 
Trp Gln Trp Lys Leu Leu Arg Met Tyr Thr Lys Pro Thr Ile Ala Met 
 65                  70                  75                  80 

gtc aat ggc tgg tgc ttc ggc ggc ggc ttc agc ccg ctg gtg gcc tgt      288 
Val Asn Gly Trp Cys Phe Gly Gly Gly Phe Ser Pro Leu Val Ala Cys 
                 85                  90                  95 

gat ctg gcc atc tgt gcc gac gag gcc acc ttt ggc ctg tcc gag atc      336 
Asp Leu Ala Ile Cys Ala Asp Glu Ala Thr Phe Gly Leu Ser Glu Ile 
            100                 105                 110 

aac tgg ggc atc ccg ccg ggc aac ctg gtg agt aag gct atg gcc gac      384 
Asn Trp Gly Ile Pro Pro Gly Asn Leu Val Ser Lys Ala Met Ala Asp 
        115                 120                 125 

acc gtg ggt cac cgc gag tcc ctt tac tac atc atg act ggc aag aca      432 
Thr Val Gly His Arg Glu Ser Leu Tyr Tyr Ile Met Thr Gly Lys Thr 
    130                 135                 140 

ttt ggc ggt cag cag gcc gcc aag atg ggg ctt gtg aac cag agt gtt      480 
Phe Gly Gly Gln Gln Ala Ala Lys Met Gly Leu Val Asn Gln Ser Val 
145                 150                 155                 160 

ccg ctg gcc gag ctg cgc agt gtc act gta gag ctg gct cag aac ctg      528 
Pro Leu Ala Glu Leu Arg Ser Val Thr Val Glu Leu Ala Gln Asn Leu 
                165                 170                 175 

ctg gac aag aac ccc gta gtg ctg cgt gcc gcc aaa ata ggc ttc aag      576 
Leu Asp Lys Asn Pro Val Val Leu Arg Ala Ala Lys Ile Gly Phe Lys 
            180                 185                 190 

cgt tgc cgc gag ctg act tgg gag cag aac gag gac tac ctg tac gcc      624 
Arg Cys Arg Glu Leu Thr Trp Glu Gln Asn Glu Asp Tyr Leu Tyr Ala 
        195                 200                 205 

aag ctc gac caa tcc cgt ttg ctc gat ccg gaa ggc ggt cgc gag cag      672 
Lys Leu Asp Gln Ser Arg Leu Leu Asp Pro Glu Gly Gly Arg Glu Gln 
    210                 215                 220 

ggc atg aag cag ttc ctt gac gag aaa agc atc aag ccg ggc ttg cag      720 
Gly Met Lys Gln Phe Leu Asp Glu Lys Ser Ile Lys Pro Gly Leu Gln 
225                 230                 235                 240 

acc tac aag cgc tga                                                  735 
Thr Tyr Lys Arg 

 
           
             26  
             244  
             PRT  
             Pseudomonas sp.  
           
            26 

Met Ser Pro Thr Leu Asn Arg Glu Met Val Glu Val Leu Glu Val Leu 
  1               5                  10                  15 

Glu Gln Asp Ala Asp Ala Arg Val Leu Val Leu Thr Gly Ala Gly Glu 
             20                  25                  30 

Ser Trp Thr Ala Gly Met Asp Leu Lys Glu Tyr Phe Arg Glu Thr Asp 
         35                  40                  45 

Ala Gly Pro Glu Ile Leu Gln Glu Lys Ile Arg Arg Glu Ala Ser Thr 
     50                  55                  60 

Trp Gln Trp Lys Leu Leu Arg Met Tyr Thr Lys Pro Thr Ile Ala Met 
 65                  70                  75                  80 

Val Asn Gly Trp Cys Phe Gly Gly Gly Phe Ser Pro Leu Val Ala Cys 
                 85                  90                  95 

Asp Leu Ala Ile Cys Ala Asp Glu Ala Thr Phe Gly Leu Ser Glu Ile 
            100                 105                 110 

Asn Trp Gly Ile Pro Pro Gly Asn Leu Val Ser Lys Ala Met Ala Asp 
        115                 120                 125 

Thr Val Gly His Arg Glu Ser Leu Tyr Tyr Ile Met Thr Gly Lys Thr 
    130                 135                 140 

Phe Gly Gly Gln Gln Ala Ala Lys Met Gly Leu Val Asn Gln Ser Val 
145                 150                 155                 160 

Pro Leu Ala Glu Leu Arg Ser Val Thr Val Glu Leu Ala Gln Asn Leu 
                165                 170                 175 

Leu Asp Lys Asn Pro Val Val Leu Arg Ala Ala Lys Ile Gly Phe Lys 
            180                 185                 190 

Arg Cys Arg Glu Leu Thr Trp Glu Gln Asn Glu Asp Tyr Leu Tyr Ala 
        195                 200                 205 

Lys Leu Asp Gln Ser Arg Leu Leu Asp Pro Glu Gly Gly Arg Glu Gln 
    210                 215                 220 

Gly Met Lys Gln Phe Leu Asp Glu Lys Ser Ile Lys Pro Gly Leu Gln 
225                 230                 235                 240 

Thr Tyr Lys Arg 

 
           
             27  
             1446  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1443)  
               product = Vanillin-Dehydrogenase“ / gene =
      ”vdh“ 
             
           
            27 

atg ttt cac gtg ccc ctg ctt att ggt ggt aag cct tgt tca gca tct       48 
Met Phe His Val Pro Leu Leu Ile Gly Gly Lys Pro Cys Ser Ala Ser 
  1               5                  10                  15 

gat gag cgc acc ttc gag cgt cgt agc ccg ctg acc gga gaa gtg gta       96 
Asp Glu Arg Thr Phe Glu Arg Arg Ser Pro Leu Thr Gly Glu Val Val 
             20                  25                  30 

tcg cgc gtc gct gct gcc agt ttg gaa gat gcg gac gcc gca gtg gcc      144 
Ser Arg Val Ala Ala Ala Ser Leu Glu Asp Ala Asp Ala Ala Val Ala 
         35                  40                  45 

gct gca cag gct gcg ttt cct gaa tgg gcg gcg ctt gct ccg agc gaa      192 
Ala Ala Gln Ala Ala Phe Pro Glu Trp Ala Ala Leu Ala Pro Ser Glu 
     50                  55                  60 

cgc cgt gcc cga ctg ctg cga gcg gcg gat ctt cta gag gac cgt tct      240 
Arg Arg Ala Arg Leu Leu Arg Ala Ala Asp Leu Leu Glu Asp Arg Ser 
 65                  70                  75                  80 

tcc gag ttc acc gcc gca gcg agt gaa act ggc gca gcg gga aac tgg      288 
Ser Glu Phe Thr Ala Ala Ala Ser Glu Thr Gly Ala Ala Gly Asn Trp 
                 85                  90                  95 

tat ggg ttt aac gtt tac ctg gcg gcg ggc atg ttg cgg gaa gcc gcg      336 
Tyr Gly Phe Asn Val Tyr Leu Ala Ala Gly Met Leu Arg Glu Ala Ala 
            100                 105                 110 

gcc atg acc aca cag att cag ggc gat gtc att ccg tcc aat gtg ccc      384 
Ala Met Thr Thr Gln Ile Gln Gly Asp Val Ile Pro Ser Asn Val Pro 
        115                 120                 125 

ggt agc ttt gcc atg gcg gtt cga cag cca tgt ggc gtg gtg ctc ggt      432 
Gly Ser Phe Ala Met Ala Val Arg Gln Pro Cys Gly Val Val Leu Gly 
    130                 135                 140 

att gcg cct tgg aat gct ccg gta atc ctt ggc gta cgg gct gtt gcg      480 
Ile Ala Pro Trp Asn Ala Pro Val Ile Leu Gly Val Arg Ala Val Ala 
145                 150                 155                 160 

atg ccg ttg gca tgc ggc aat acc gtg gtg ttg aaa agc tct gag ctg      528 
Met Pro Leu Ala Cys Gly Asn Thr Val Val Leu Lys Ser Ser Glu Leu 
                165                 170                 175 

agt ccc ttt acc cat cgc ctg att ggt cag gtg ttg cat gat gct ggt      576 
Ser Pro Phe Thr His Arg Leu Ile Gly Gln Val Leu His Asp Ala Gly 
            180                 185                 190 

ctg ggg gat ggc gtg gtg aat gtc atc agc aat gcc ccg caa gac gct      624 
Leu Gly Asp Gly Val Val Asn Val Ile Ser Asn Ala Pro Gln Asp Ala 
        195                 200                 205 

cct gcg gtg gtg gag cga ctg att gca aat cct gcg gta cgt cga gtg      672 
Pro Ala Val Val Glu Arg Leu Ile Ala Asn Pro Ala Val Arg Arg Val 
    210                 215                 220 

aac ttc acc ggt tcg acc cac gtt gga cgg atc att ggt gag ctg tct      720 
Asn Phe Thr Gly Ser Thr His Val Gly Arg Ile Ile Gly Glu Leu Ser 
225                 230                 235                 240 

gcg cgt cat ctg aag cct gct gtg ctg gaa tta ggt ggt aag gct ccg      768 
Ala Arg His Leu Lys Pro Ala Val Leu Glu Leu Gly Gly Lys Ala Pro 
                245                 250                 255 

ttc ttg gtc ttg gac gat gcc gac ctc gat gcg gcg gtc gaa gcg gcg      816 
Phe Leu Val Leu Asp Asp Ala Asp Leu Asp Ala Ala Val Glu Ala Ala 
            260                 265                 270 

gcc ttt ggt gcc tac ttc aat cag ggt caa atc tgc atg tcc act gag      864 
Ala Phe Gly Ala Tyr Phe Asn Gln Gly Gln Ile Cys Met Ser Thr Glu 
        275                 280                 285 

cgt ctg att gtg aca gca gtc gca gac gcc ttt gtt gaa aag ctg gcg      912 
Arg Leu Ile Val Thr Ala Val Ala Asp Ala Phe Val Glu Lys Leu Ala 
    290                 295                 300 

agg aag gtc gcc aca ctg cgt gct ggc gat cct aat gat ccg caa tcg      960 
Arg Lys Val Ala Thr Leu Arg Ala Gly Asp Pro Asn Asp Pro Gln Ser 
305                 310                 315                 320 

gtc ttg ggt tcg ttg att gat gcc aat gca ggt caa cgc atc cag gtt     1008 
Val Leu Gly Ser Leu Ile Asp Ala Asn Ala Gly Gln Arg Ile Gln Val 
                325                 330                 335 

ctg gtc gat gat gcg ctc gca aaa ggc gcg cgg cag gtc gtc ggt ggt     1056 
Leu Val Asp Asp Ala Leu Ala Lys Gly Ala Arg Gln Val Val Gly Gly 
            340                 345                 350 

ggc tta gat ggc agc atc atg cag ccg atg ctg ctt gat cag gtc act     1104 
Gly Leu Asp Gly Ser Ile Met Gln Pro Met Leu Leu Asp Gln Val Thr 
        355                 360                 365 

gaa gag atg cgg ctc tac cgt gag gag tcc ttt ggc cct gtt gcc gtt     1152 
Glu Glu Met Arg Leu Tyr Arg Glu Glu Ser Phe Gly Pro Val Ala Val 
    370                 375                 380 

gtc ttg cgc ggc gat ggt gat gaa gaa ctg ctg cgt ctt gcc aac gat     1200 
Val Leu Arg Gly Asp Gly Asp Glu Glu Leu Leu Arg Leu Ala Asn Asp 
385                 390                 395                 400 

tcg gag ttt ggt ctt tcg gcc gcc att ttc agc cgt gac gtc tcg cgc     1248 
Ser Glu Phe Gly Leu Ser Ala Ala Ile Phe Ser Arg Asp Val Ser Arg 
                405                 410                 415 

gca atg gaa ttg gcc cag cgc gtc gat tcg ggc att tgc cat atc aat     1296 
Ala Met Glu Leu Ala Gln Arg Val Asp Ser Gly Ile Cys His Ile Asn 
            420                 425                 430 

gga ccg act gtg cat gac gag gct cag atg cca ttc ggt ggg gtg aag     1344 
Gly Pro Thr Val His Asp Glu Ala Gln Met Pro Phe Gly Gly Val Lys 
        435                 440                 445 

tcc agc ggc tac ggc agc ttc ggc agt cga gca tcg att gag cac ttt     1392 
Ser Ser Gly Tyr Gly Ser Phe Gly Ser Arg Ala Ser Ile Glu His Phe 
    450                 455                 460 

acc cag ctg cgc tgg ctg acc att cag aat ggc ccg cgg cac tat cca     1440 
Thr Gln Leu Arg Trp Leu Thr Ile Gln Asn Gly Pro Arg His Tyr Pro 
465                 470                 475                 480 

atc taa                                                             1446 
Ile 

 
           
             28  
             481  
             PRT  
             Pseudomonas sp.  
           
            28 

Met Phe His Val Pro Leu Leu Ile Gly Gly Lys Pro Cys Ser Ala Ser 
  1               5                  10                  15 

Asp Glu Arg Thr Phe Glu Arg Arg Ser Pro Leu Thr Gly Glu Val Val 
             20                  25                  30 

Ser Arg Val Ala Ala Ala Ser Leu Glu Asp Ala Asp Ala Ala Val Ala 
         35                  40                  45 

Ala Ala Gln Ala Ala Phe Pro Glu Trp Ala Ala Leu Ala Pro Ser Glu 
     50                  55                  60 

Arg Arg Ala Arg Leu Leu Arg Ala Ala Asp Leu Leu Glu Asp Arg Ser 
 65                  70                  75                  80 

Ser Glu Phe Thr Ala Ala Ala Ser Glu Thr Gly Ala Ala Gly Asn Trp 
                 85                  90                  95 

Tyr Gly Phe Asn Val Tyr Leu Ala Ala Gly Met Leu Arg Glu Ala Ala 
            100                 105                 110 

Ala Met Thr Thr Gln Ile Gln Gly Asp Val Ile Pro Ser Asn Val Pro 
        115                 120                 125 

Gly Ser Phe Ala Met Ala Val Arg Gln Pro Cys Gly Val Val Leu Gly 
    130                 135                 140 

Ile Ala Pro Trp Asn Ala Pro Val Ile Leu Gly Val Arg Ala Val Ala 
145                 150                 155                 160 

Met Pro Leu Ala Cys Gly Asn Thr Val Val Leu Lys Ser Ser Glu Leu 
                165                 170                 175 

Ser Pro Phe Thr His Arg Leu Ile Gly Gln Val Leu His Asp Ala Gly 
            180                 185                 190 

Leu Gly Asp Gly Val Val Asn Val Ile Ser Asn Ala Pro Gln Asp Ala 
        195                 200                 205 

Pro Ala Val Val Glu Arg Leu Ile Ala Asn Pro Ala Val Arg Arg Val 
    210                 215                 220 

Asn Phe Thr Gly Ser Thr His Val Gly Arg Ile Ile Gly Glu Leu Ser 
225                 230                 235                 240 

Ala Arg His Leu Lys Pro Ala Val Leu Glu Leu Gly Gly Lys Ala Pro 
                245                 250                 255 

Phe Leu Val Leu Asp Asp Ala Asp Leu Asp Ala Ala Val Glu Ala Ala 
            260                 265                 270 

Ala Phe Gly Ala Tyr Phe Asn Gln Gly Gln Ile Cys Met Ser Thr Glu 
        275                 280                 285 

Arg Leu Ile Val Thr Ala Val Ala Asp Ala Phe Val Glu Lys Leu Ala 
    290                 295                 300 

Arg Lys Val Ala Thr Leu Arg Ala Gly Asp Pro Asn Asp Pro Gln Ser 
305                 310                 315                 320 

Val Leu Gly Ser Leu Ile Asp Ala Asn Ala Gly Gln Arg Ile Gln Val 
                325                 330                 335 

Leu Val Asp Asp Ala Leu Ala Lys Gly Ala Arg Gln Val Val Gly Gly 
            340                 345                 350 

Gly Leu Asp Gly Ser Ile Met Gln Pro Met Leu Leu Asp Gln Val Thr 
        355                 360                 365 

Glu Glu Met Arg Leu Tyr Arg Glu Glu Ser Phe Gly Pro Val Ala Val 
    370                 375                 380 

Val Leu Arg Gly Asp Gly Asp Glu Glu Leu Leu Arg Leu Ala Asn Asp 
385                 390                 395                 400 

Ser Glu Phe Gly Leu Ser Ala Ala Ile Phe Ser Arg Asp Val Ser Arg 
                405                 410                 415 

Ala Met Glu Leu Ala Gln Arg Val Asp Ser Gly Ile Cys His Ile Asn 
            420                 425                 430 

Gly Pro Thr Val His Asp Glu Ala Gln Met Pro Phe Gly Gly Val Lys 
        435                 440                 445 

Ser Ser Gly Tyr Gly Ser Phe Gly Ser Arg Ala Ser Ile Glu His Phe 
    450                 455                 460 

Thr Gln Leu Arg Trp Leu Thr Ile Gln Asn Gly Pro Arg His Tyr Pro 
465                 470                 475                 480 

Ile 

 
           
             29  
             1770  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1767)  
               product = ”Ferulasaeure-CoA-Synthetase“ / 
      gene =”fcs“ 
             
           
            29 

atg cgt tct ctc gag gcg ctt ctt ccc ttc ccg ggt cga att ctt gag       48 
Met Arg Ser Leu Glu Ala Leu Leu Pro Phe Pro Gly Arg Ile Leu Glu 
  1               5                  10                  15 

cgt ctc gag cat tgg gct aag acc cgt cca gaa caa acc tgc gtt gct       96 
Arg Leu Glu His Trp Ala Lys Thr Arg Pro Glu Gln Thr Cys Val Ala 
             20                  25                  30 

gcc agg gcg gca aat ggg gaa tgg cgt cgt atc agc tac gcg gaa atg      144 
Ala Arg Ala Ala Asn Gly Glu Trp Arg Arg Ile Ser Tyr Ala Glu Met 
         35                  40                  45 

ttc cac aac gtc cgc gcc atc gca cag agc ttg ctt cct tac gga cta      192 
Phe His Asn Val Arg Ala Ile Ala Gln Ser Leu Leu Pro Tyr Gly Leu 
     50                  55                  60 

tcg gca gag cgt ccg ctg ctt atc gtc tct gga aat gac ctg gaa cat      240 
Ser Ala Glu Arg Pro Leu Leu Ile Val Ser Gly Asn Asp Leu Glu His 
 65                  70                  75                  80 

ctt cag ctg gca ttt ggg gct atg tat gcg ggc att ccc tat tgc ccg      288 
Leu Gln Leu Ala Phe Gly Ala Met Tyr Ala Gly Ile Pro Tyr Cys Pro 
                 85                  90                  95 

gtg tct cct gct tat tca ctg ctg tcg caa gat ttg gcg aag ctg cgt      336 
Val Ser Pro Ala Tyr Ser Leu Leu Ser Gln Asp Leu Ala Lys Leu Arg 
            100                 105                 110 

cac atc gta ggt ctt ctg caa ccg gga ctg gtc ttt gct gcc gat gca      384 
His Ile Val Gly Leu Leu Gln Pro Gly Leu Val Phe Ala Ala Asp Ala 
        115                 120                 125 

gca cct ttc cag cgc gca att gag acc att ctg ccg gac gac gtg ccc      432 
Ala Pro Phe Gln Arg Ala Ile Glu Thr Ile Leu Pro Asp Asp Val Pro 
    130                 135                 140 

gca atc ttc act cga ggc gaa ttg gcc ggg cgg cgc acg gtg agt ttt      480 
Ala Ile Phe Thr Arg Gly Glu Leu Ala Gly Arg Arg Thr Val Ser Phe 
145                 150                 155                 160 

gac agc ctg ctg gag cag cct ggt ggg att gag gca gat aat gcc ttt      528 
Asp Ser Leu Leu Glu Gln Pro Gly Gly Ile Glu Ala Asp Asn Ala Phe 
                165                 170                 175 

gcg gca act ggc ccc gat acg att gcc aag ttc ttg ttc act tct ggc      576 
Ala Ala Thr Gly Pro Asp Thr Ile Ala Lys Phe Leu Phe Thr Ser Gly 
            180                 185                 190 

tct acc aaa ctg cct aag gcg gtg ccg act act cag cga atg ctc tgc      624 
Ser Thr Lys Leu Pro Lys Ala Val Pro Thr Thr Gln Arg Met Leu Cys 
        195                 200                 205 

gcc aat cag cag atg ctt ctg caa act ttc ccg gtt ttt ggt gaa gag      672 
Ala Asn Gln Gln Met Leu Leu Gln Thr Phe Pro Val Phe Gly Glu Glu 
    210                 215                 220 

ccg ccg gtg ctg gtg gac tgg ttg ccg tgg aac cac acc ttc ggc ggc      720 
Pro Pro Val Leu Val Asp Trp Leu Pro Trp Asn His Thr Phe Gly Gly 
225                 230                 235                 240 

agc cac aac atc ggc atc gtg ttg tac aac ggc ggc acg tac tac ctt      768 
Ser His Asn Ile Gly Ile Val Leu Tyr Asn Gly Gly Thr Tyr Tyr Leu 
                245                 250                 255 

gac gac ggt aaa cca acc gcc caa ggg ttc gcc gag acg ctt cgc aac      816 
Asp Asp Gly Lys Pro Thr Ala Gln Gly Phe Ala Glu Thr Leu Arg Asn 
            260                 265                 270 

ttg agc gaa atc tct ccc act gcg tac ctc act gtg ccg aaa ggc tgg      864 
Leu Ser Glu Ile Ser Pro Thr Ala Tyr Leu Thr Val Pro Lys Gly Trp 
        275                 280                 285 

gag gaa tta gtg ggt gcc ctt gag cga gac agt acc ctg cgc gaa cgc      912 
Glu Glu Leu Val Gly Ala Leu Glu Arg Asp Ser Thr Leu Arg Glu Arg 
    290                 295                 300 

ttc ttc gct cgc atg aag ctg ttc ttc ttc gcg gcg gct ggg ttg tcg      960 
Phe Phe Ala Arg Met Lys Leu Phe Phe Phe Ala Ala Ala Gly Leu Ser 
305                 310                 315                 320 

caa ggg atc tgg gat cgt ttg gac cgg gtc gct gaa cag cac tgt ggt     1008 
Gln Gly Ile Trp Asp Arg Leu Asp Arg Val Ala Glu Gln His Cys Gly 
                325                 330                 335 

gag cgc att cgc atg atg gcg ggt ctg ggc atg acg gag act gct cct     1056 
Glu Arg Ile Arg Met Met Ala Gly Leu Gly Met Thr Glu Thr Ala Pro 
            340                 345                 350 

tcc tgc act ttt acc acc gga ccg ctg tcg atg gct ggt tac att ggg     1104 
Ser Cys Thr Phe Thr Thr Gly Pro Leu Ser Met Ala Gly Tyr Ile Gly 
        355                 360                 365 

ctg cca gcg cct ggc tgc gag gtc aag ctc gtt ccg gtc gat ggg aaa     1152 
Leu Pro Ala Pro Gly Cys Glu Val Lys Leu Val Pro Val Asp Gly Lys 
    370                 375                 380 

ttg gaa ggg cgt ttc cat ggt ccg cac gtc atg agc ggc tac tgg cgt     1200 
Leu Glu Gly Arg Phe His Gly Pro His Val Met Ser Gly Tyr Trp Arg 
385                 390                 395                 400 

gct cct gaa caa aat gcc caa gcg ttc gac gag gaa ggc tat tac tgc     1248 
Ala Pro Glu Gln Asn Ala Gln Ala Phe Asp Glu Glu Gly Tyr Tyr Cys 
                405                 410                 415 

tcc ggt gat gcc atc aaa ttg gca gat cct gcc gat cct cag aaa ggt     1296 
Ser Gly Asp Ala Ile Lys Leu Ala Asp Pro Ala Asp Pro Gln Lys Gly 
            420                 425                 430 

ctg atg ttt gac ggt cga att gct gaa gac ttc aag ctg tcc tca ggg     1344 
Leu Met Phe Asp Gly Arg Ile Ala Glu Asp Phe Lys Leu Ser Ser Gly 
        435                 440                 445 

gta ttt gtc agc gtt ggg cca ttg cgc acg cgg gcg gtt ctg gaa ggc     1392 
Val Phe Val Ser Val Gly Pro Leu Arg Thr Arg Ala Val Leu Glu Gly 
    450                 455                 460 

ggc tct tac gtc ctg gac gta gtg gtt gct gct cct gat cgt gaa tgc     1440 
Gly Ser Tyr Val Leu Asp Val Val Val Ala Ala Pro Asp Arg Glu Cys 
465                 470                 475                 480 

ctt gga ttg ctc gtg ttt ccg cgt ctt ctc gac tgc cgt gcc ttg tcg     1488 
Leu Gly Leu Leu Val Phe Pro Arg Leu Leu Asp Cys Arg Ala Leu Ser 
                485                 490                 495 

ggg cta gga aaa gag gcg tcg gac gcc gag gtg ctt gcc agt gag ccg     1536 
Gly Leu Gly Lys Glu Ala Ser Asp Ala Glu Val Leu Ala Ser Glu Pro 
            500                 505                 510 

gtt cgg gcc tgg ttt gct gac tgg ctc aaa cga ctc aat cga gaa gca     1584 
Val Arg Ala Trp Phe Ala Asp Trp Leu Lys Arg Leu Asn Arg Glu Ala 
        515                 520                 525 

act ggc aat gcc agt cgc atc atg tgg gta ggg ctc ctc gat acg ccg     1632 
Thr Gly Asn Ala Ser Arg Ile Met Trp Val Gly Leu Leu Asp Thr Pro 
    530                 535                 540 

ccg tcg att gat aag ggc gag gtc act gac aag ggc tcg atc aac cag     1680 
Pro Ser Ile Asp Lys Gly Glu Val Thr Asp Lys Gly Ser Ile Asn Gln 
545                 550                 555                 560 

cgc gct gtt ttg caa tgg cgg tcg gcg aaa gtt gat gcg ctg tat cgt     1728 
Arg Ala Val Leu Gln Trp Arg Ser Ala Lys Val Asp Ala Leu Tyr Arg 
                565                 570                 575 

ggt gaa gat caa tcc atg ctg cgt gac gag gcc aca ctg tga             1770 
Gly Glu Asp Gln Ser Met Leu Arg Asp Glu Ala Thr Leu 
            580                 585 

 
           
             30  
             589  
             PRT  
             Pseudomonas sp.  
           
            30 

Met Arg Ser Leu Glu Ala Leu Leu Pro Phe Pro Gly Arg Ile Leu Glu 
  1               5                  10                  15 

Arg Leu Glu His Trp Ala Lys Thr Arg Pro Glu Gln Thr Cys Val Ala 
             20                  25                  30 

Ala Arg Ala Ala Asn Gly Glu Trp Arg Arg Ile Ser Tyr Ala Glu Met 
         35                  40                  45 

Phe His Asn Val Arg Ala Ile Ala Gln Ser Leu Leu Pro Tyr Gly Leu 
     50                  55                  60 

Ser Ala Glu Arg Pro Leu Leu Ile Val Ser Gly Asn Asp Leu Glu His 
 65                  70                  75                  80 

Leu Gln Leu Ala Phe Gly Ala Met Tyr Ala Gly Ile Pro Tyr Cys Pro 
                 85                  90                  95 

Val Ser Pro Ala Tyr Ser Leu Leu Ser Gln Asp Leu Ala Lys Leu Arg 
            100                 105                 110 

His Ile Val Gly Leu Leu Gln Pro Gly Leu Val Phe Ala Ala Asp Ala 
        115                 120                 125 

Ala Pro Phe Gln Arg Ala Ile Glu Thr Ile Leu Pro Asp Asp Val Pro 
    130                 135                 140 

Ala Ile Phe Thr Arg Gly Glu Leu Ala Gly Arg Arg Thr Val Ser Phe 
145                 150                 155                 160 

Asp Ser Leu Leu Glu Gln Pro Gly Gly Ile Glu Ala Asp Asn Ala Phe 
                165                 170                 175 

Ala Ala Thr Gly Pro Asp Thr Ile Ala Lys Phe Leu Phe Thr Ser Gly 
            180                 185                 190 

Ser Thr Lys Leu Pro Lys Ala Val Pro Thr Thr Gln Arg Met Leu Cys 
        195                 200                 205 

Ala Asn Gln Gln Met Leu Leu Gln Thr Phe Pro Val Phe Gly Glu Glu 
    210                 215                 220 

Pro Pro Val Leu Val Asp Trp Leu Pro Trp Asn His Thr Phe Gly Gly 
225                 230                 235                 240 

Ser His Asn Ile Gly Ile Val Leu Tyr Asn Gly Gly Thr Tyr Tyr Leu 
                245                 250                 255 

Asp Asp Gly Lys Pro Thr Ala Gln Gly Phe Ala Glu Thr Leu Arg Asn 
            260                 265                 270 

Leu Ser Glu Ile Ser Pro Thr Ala Tyr Leu Thr Val Pro Lys Gly Trp 
        275                 280                 285 

Glu Glu Leu Val Gly Ala Leu Glu Arg Asp Ser Thr Leu Arg Glu Arg 
    290                 295                 300 

Phe Phe Ala Arg Met Lys Leu Phe Phe Phe Ala Ala Ala Gly Leu Ser 
305                 310                 315                 320 

Gln Gly Ile Trp Asp Arg Leu Asp Arg Val Ala Glu Gln His Cys Gly 
                325                 330                 335 

Glu Arg Ile Arg Met Met Ala Gly Leu Gly Met Thr Glu Thr Ala Pro 
            340                 345                 350 

Ser Cys Thr Phe Thr Thr Gly Pro Leu Ser Met Ala Gly Tyr Ile Gly 
        355                 360                 365 

Leu Pro Ala Pro Gly Cys Glu Val Lys Leu Val Pro Val Asp Gly Lys 
    370                 375                 380 

Leu Glu Gly Arg Phe His Gly Pro His Val Met Ser Gly Tyr Trp Arg 
385                 390                 395                 400 

Ala Pro Glu Gln Asn Ala Gln Ala Phe Asp Glu Glu Gly Tyr Tyr Cys 
                405                 410                 415 

Ser Gly Asp Ala Ile Lys Leu Ala Asp Pro Ala Asp Pro Gln Lys Gly 
            420                 425                 430 

Leu Met Phe Asp Gly Arg Ile Ala Glu Asp Phe Lys Leu Ser Ser Gly 
        435                 440                 445 

Val Phe Val Ser Val Gly Pro Leu Arg Thr Arg Ala Val Leu Glu Gly 
    450                 455                 460 

Gly Ser Tyr Val Leu Asp Val Val Val Ala Ala Pro Asp Arg Glu Cys 
465                 470                 475                 480 

Leu Gly Leu Leu Val Phe Pro Arg Leu Leu Asp Cys Arg Ala Leu Ser 
                485                 490                 495 

Gly Leu Gly Lys Glu Ala Ser Asp Ala Glu Val Leu Ala Ser Glu Pro 
            500                 505                 510 

Val Arg Ala Trp Phe Ala Asp Trp Leu Lys Arg Leu Asn Arg Glu Ala 
        515                 520                 525 

Thr Gly Asn Ala Ser Arg Ile Met Trp Val Gly Leu Leu Asp Thr Pro 
    530                 535                 540 

Pro Ser Ile Asp Lys Gly Glu Val Thr Asp Lys Gly Ser Ile Asn Gln 
545                 550                 555                 560 

Arg Ala Val Leu Gln Trp Arg Ser Ala Lys Val Asp Ala Leu Tyr Arg 
                565                 570                 575 

Gly Glu Asp Gln Ser Met Leu Arg Asp Glu Ala Thr Leu 
            580                 585 

 
           
             31  
             1296  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1293)  
               product = ”beta-Ketothiolase“ / gene = ”aat“ 
             
           
            31 

atg agt tgg tca ggg ggg gct tac tcg gcg ttt tcc gac act gcg ttg       48 
Met Ser Trp Ser Gly Gly Ala Tyr Ser Ala Phe Ser Asp Thr Ala Leu 
  1               5                  10                  15 

gtt gcg gca gtg cgc acc ccc tgg att gat tgc ggg ggt gcc ctg tcg       96 
Val Ala Ala Val Arg Thr Pro Trp Ile Asp Cys Gly Gly Ala Leu Ser 
             20                  25                  30 

ctg gtg tcg cct atc gac tta ggg gta aag gtc gct cgc gaa gtt ctg      144 
Leu Val Ser Pro Ile Asp Leu Gly Val Lys Val Ala Arg Glu Val Leu 
         35                  40                  45 

atg cgt gcg tcg ctt gaa cca caa atg gtc gat agc gta ctc gca ggc      192 
Met Arg Ala Ser Leu Glu Pro Gln Met Val Asp Ser Val Leu Ala Gly 
     50                  55                  60 

tct atg gct caa gca agc ttt gat gct tac ctg ctc ccg cgg cac att      240 
Ser Met Ala Gln Ala Ser Phe Asp Ala Tyr Leu Leu Pro Arg His Ile 
 65                  70                  75                  80 

ggc ttg tac agc ggt gtt ccc aag tcg gtt ccg gcc ttg ggg gtg cag      288 
Gly Leu Tyr Ser Gly Val Pro Lys Ser Val Pro Ala Leu Gly Val Gln 
                 85                  90                  95 

cgc att tgc ggc aca ggc ttc gaa ctg ctt cgg cag gcc ggc gag cag      336 
Arg Ile Cys Gly Thr Gly Phe Glu Leu Leu Arg Gln Ala Gly Glu Gln 
            100                 105                 110 

att tcc caa ggc gct gat cac gtg ctg tgt gtc gcg gca gag tcc atg      384 
Ile Ser Gln Gly Ala Asp His Val Leu Cys Val Ala Ala Glu Ser Met 
        115                 120                 125 

tcg cgt aac ccc atc gcg tcg tat aca cac cgg ggc ggg ttc cgc ctc      432 
Ser Arg Asn Pro Ile Ala Ser Tyr Thr His Arg Gly Gly Phe Arg Leu 
    130                 135                 140 

ggt gcg ccc gtt gag ttc aag gat ttt ttg tgg gag gca ttg ttt gat      480 
Gly Ala Pro Val Glu Phe Lys Asp Phe Leu Trp Glu Ala Leu Phe Asp 
145                 150                 155                 160 

cct gct cca gga ctc gac atg atc gct acc gca gaa aac ctg gcg cgc      528 
Pro Ala Pro Gly Leu Asp Met Ile Ala Thr Ala Glu Asn Leu Ala Arg 
                165                 170                 175 

ctg tac gga atc acc agg gga gaa gct aat tcc tac gcg gta agc agc      576 
Leu Tyr Gly Ile Thr Arg Gly Glu Ala Asn Ser Tyr Ala Val Ser Ser 
            180                 185                 190 

ttc gag cgc gca ttg agg gcg caa gag gag aaa tgg att gac caa gag      624 
Phe Glu Arg Ala Leu Arg Ala Gln Glu Glu Lys Trp Ile Asp Gln Glu 
        195                 200                 205 

atc gtg gct gtt acg gat gaa cag ttc gat tta gag ggc tac aac agt      672 
Ile Val Ala Val Thr Asp Glu Gln Phe Asp Leu Glu Gly Tyr Asn Ser 
    210                 215                 220 

cga gca att gaa ctg cct cgg aag gca aaa ttg ttg atc gtg aca gtc      720 
Arg Ala Ile Glu Leu Pro Arg Lys Ala Lys Leu Leu Ile Val Thr Val 
225                 230                 235                 240 

atc cgc ggc cta gca gtc ttt gaa gcc ctt tcc cga ttg aag cct gtt      768 
Ile Arg Gly Leu Ala Val Phe Glu Ala Leu Ser Arg Leu Lys Pro Val 
                245                 250                 255 

cat tct ggc ggg gtg cag act gcg ggc aac agc tgt gcc gta gtg gac      816 
His Ser Gly Gly Val Gln Thr Ala Gly Asn Ser Cys Ala Val Val Asp 
            260                 265                 270 

ggc gcc gcg gcg gct ttg gtg gct cga gag tcg tct gcg aca cag ccg      864 
Gly Ala Ala Ala Ala Leu Val Ala Arg Glu Ser Ser Ala Thr Gln Pro 
        275                 280                 285 

gtc ttg gct agg ata ctg gct acc tcc gta gtc ggg atc gag ccc gag      912 
Val Leu Ala Arg Ile Leu Ala Thr Ser Val Val Gly Ile Glu Pro Glu 
    290                 295                 300 

cat atg ggg ctc ggc cct gcg ccc gcg att cgc ctg ctg ctt gcg cgt      960 
His Met Gly Leu Gly Pro Ala Pro Ala Ile Arg Leu Leu Leu Ala Arg 
305                 310                 315                 320 

agt gat ctt agt ttg agg gat atc gac ctc ttt gag ata aac gag gcg     1008 
Ser Asp Leu Ser Leu Arg Asp Ile Asp Leu Phe Glu Ile Asn Glu Ala 
                325                 330                 335 

cag gcc gcc caa gtt cta gcg gta cag cat gaa ttg ggt att gag cac     1056 
Gln Ala Ala Gln Val Leu Ala Val Gln His Glu Leu Gly Ile Glu His 
            340                 345                 350 

tca aaa ctt aat att tgg ggc ggg gcc att gca ctt gga cac ccg ctt     1104 
Ser Lys Leu Asn Ile Trp Gly Gly Ala Ile Ala Leu Gly His Pro Leu 
        355                 360                 365 

gcc gcg acc gga ttg cgt ctc tgc atg acc ctc gct cac caa ttg caa     1152 
Ala Ala Thr Gly Leu Arg Leu Cys Met Thr Leu Ala His Gln Leu Gln 
    370                 375                 380 

gct aat aac ttt cga tat gga att gcc tcg gca tgc att ggt ggg gga     1200 
Ala Asn Asn Phe Arg Tyr Gly Ile Ala Ser Ala Cys Ile Gly Gly Gly 
385                 390                 395                 400 

cag ggg atg gcg gtt ctt tta gag aat ccc cac ttc ggt tcg tcc tct     1248 
Gln Gly Met Ala Val Leu Leu Glu Asn Pro His Phe Gly Ser Ser Ser 
                405                 410                 415 

gca cga agt tcg atg att aac aga gtt gac cac tat cca ctg agc taa     1296 
Ala Arg Ser Ser Met Ile Asn Arg Val Asp His Tyr Pro Leu Ser 
            420                 425                 430 

 
           
             32  
             431  
             PRT  
             Pseudomonas sp.  
           
            32 

Met Ser Trp Ser Gly Gly Ala Tyr Ser Ala Phe Ser Asp Thr Ala Leu 
  1               5                  10                  15 

Val Ala Ala Val Arg Thr Pro Trp Ile Asp Cys Gly Gly Ala Leu Ser 
             20                  25                  30 

Leu Val Ser Pro Ile Asp Leu Gly Val Lys Val Ala Arg Glu Val Leu 
         35                  40                  45 

Met Arg Ala Ser Leu Glu Pro Gln Met Val Asp Ser Val Leu Ala Gly 
     50                  55                  60 

Ser Met Ala Gln Ala Ser Phe Asp Ala Tyr Leu Leu Pro Arg His Ile 
 65                  70                  75                  80 

Gly Leu Tyr Ser Gly Val Pro Lys Ser Val Pro Ala Leu Gly Val Gln 
                 85                  90                  95 

Arg Ile Cys Gly Thr Gly Phe Glu Leu Leu Arg Gln Ala Gly Glu Gln 
            100                 105                 110 

Ile Ser Gln Gly Ala Asp His Val Leu Cys Val Ala Ala Glu Ser Met 
        115                 120                 125 

Ser Arg Asn Pro Ile Ala Ser Tyr Thr His Arg Gly Gly Phe Arg Leu 
    130                 135                 140 

Gly Ala Pro Val Glu Phe Lys Asp Phe Leu Trp Glu Ala Leu Phe Asp 
145                 150                 155                 160 

Pro Ala Pro Gly Leu Asp Met Ile Ala Thr Ala Glu Asn Leu Ala Arg 
                165                 170                 175 

Leu Tyr Gly Ile Thr Arg Gly Glu Ala Asn Ser Tyr Ala Val Ser Ser 
            180                 185                 190 

Phe Glu Arg Ala Leu Arg Ala Gln Glu Glu Lys Trp Ile Asp Gln Glu 
        195                 200                 205 

Ile Val Ala Val Thr Asp Glu Gln Phe Asp Leu Glu Gly Tyr Asn Ser 
    210                 215                 220 

Arg Ala Ile Glu Leu Pro Arg Lys Ala Lys Leu Leu Ile Val Thr Val 
225                 230                 235                 240 

Ile Arg Gly Leu Ala Val Phe Glu Ala Leu Ser Arg Leu Lys Pro Val 
                245                 250                 255 

His Ser Gly Gly Val Gln Thr Ala Gly Asn Ser Cys Ala Val Val Asp 
            260                 265                 270 

Gly Ala Ala Ala Ala Leu Val Ala Arg Glu Ser Ser Ala Thr Gln Pro 
        275                 280                 285 

Val Leu Ala Arg Ile Leu Ala Thr Ser Val Val Gly Ile Glu Pro Glu 
    290                 295                 300 

His Met Gly Leu Gly Pro Ala Pro Ala Ile Arg Leu Leu Leu Ala Arg 
305                 310                 315                 320 

Ser Asp Leu Ser Leu Arg Asp Ile Asp Leu Phe Glu Ile Asn Glu Ala 
                325                 330                 335 

Gln Ala Ala Gln Val Leu Ala Val Gln His Glu Leu Gly Ile Glu His 
            340                 345                 350 

Ser Lys Leu Asn Ile Trp Gly Gly Ala Ile Ala Leu Gly His Pro Leu 
        355                 360                 365 

Ala Ala Thr Gly Leu Arg Leu Cys Met Thr Leu Ala His Gln Leu Gln 
    370                 375                 380 

Ala Asn Asn Phe Arg Tyr Gly Ile Ala Ser Ala Cys Ile Gly Gly Gly 
385                 390                 395                 400 

Gln Gly Met Ala Val Leu Leu Glu Asn Pro His Phe Gly Ser Ser Ser 
                405                 410                 415 

Ala Arg Ser Ser Met Ile Asn Arg Val Asp His Tyr Pro Leu Ser 
            420                 425                 430 

 
           
             33  
             1596  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1593)  
               product = ”Chemotaxis-Protein“ / gene = ”mac“ 
             
           
            33 

atg att agt ttc gct cgt atg gca gaa agt tta gga gtc cag gct aaa       48 
Met Ile Ser Phe Ala Arg Met Ala Glu Ser Leu Gly Val Gln Ala Lys 
  1               5                  10                  15 

ctt gcc ctt gcc ttc gca ctc gta tta tgt gtc ggg ctg att gtt acc       96 
Leu Ala Leu Ala Phe Ala Leu Val Leu Cys Val Gly Leu Ile Val Thr 
             20                  25                  30 

ggc acg ggt ttc tac agt gta cat acc ttg tca ggg ttg gtg gaa aag      144 
Gly Thr Gly Phe Tyr Ser Val His Thr Leu Ser Gly Leu Val Glu Lys 
         35                  40                  45 

agc gcg ata gct ggt gag ttg cgg gcg aaa att cag gaa ctg aag gtt      192 
Ser Ala Ile Ala Gly Glu Leu Arg Ala Lys Ile Gln Glu Leu Lys Val 
     50                  55                  60 

ctg gag cag cgc gcc tta ttc atc gcc gat gaa ggg tcg ctg aag cag      240 
Leu Glu Gln Arg Ala Leu Phe Ile Ala Asp Glu Gly Ser Leu Lys Gln 
 65                  70                  75                  80 

cgc tcg atc ctc cta agt cag gtg ata gct gaa gtt aat gat gct ata      288 
Arg Ser Ile Leu Leu Ser Gln Val Ile Ala Glu Val Asn Asp Ala Ile 
                 85                  90                  95 

gat att ttt gac ttt cag cgc gga cga tct gag tta ctt aaa ttc gct      336 
Asp Ile Phe Asp Phe Gln Arg Gly Arg Ser Glu Leu Leu Lys Phe Ala 
            100                 105                 110 

gct tct tcg cgc gaa gca agt tac tcc att gag gtc ggt agt aac gct      384 
Ala Ser Ser Arg Glu Ala Ser Tyr Ser Ile Glu Val Gly Ser Asn Ala 
        115                 120                 125 

gcg gcc gat aag ttg cag tcg ggc gaa cca agt gac gca ttg atg gtt      432 
Ala Ala Asp Lys Leu Gln Ser Gly Glu Pro Ser Asp Ala Leu Met Val 
    130                 135                 140 

gcc gat aaa aag ctg aat gtt gag tat gag caa ttg agt tct gct gtg      480 
Ala Asp Lys Lys Leu Asn Val Glu Tyr Glu Gln Leu Ser Ser Ala Val 
145                 150                 155                 160 

aat gca ctg atg ggg cat tta att gag gat cag aat gaa aaa gtt cca      528 
Asn Ala Leu Met Gly His Leu Ile Glu Asp Gln Asn Glu Lys Val Pro 
                165                 170                 175 

cta atc tac tat atg ctt ggc ggc gta act ttg ttt acg atg ctc atg      576 
Leu Ile Tyr Tyr Met Leu Gly Gly Val Thr Leu Phe Thr Met Leu Met 
            180                 185                 190 

agt gct tat tcg gtc tgg ttc att tcg cgt cag tta gtt ccg cca tta      624 
Ser Ala Tyr Ser Val Trp Phe Ile Ser Arg Gln Leu Val Pro Pro Leu 
        195                 200                 205 

aag tcg acg gtg cag ctt gcc gag cgg att gca tca ggc gac ttg gct      672 
Lys Ser Thr Val Gln Leu Ala Glu Arg Ile Ala Ser Gly Asp Leu Ala 
    210                 215                 220 

gat gtc ggg gac agc agg cgc aag gat gaa atc ggt cag ttg caa agt      720 
Asp Val Gly Asp Ser Arg Arg Lys Asp Glu Ile Gly Gln Leu Gln Ser 
225                 230                 235                 240 

gca act agg cgg atg gcg att gga ctg cgt aat ctg gtc ggt gat att      768 
Ala Thr Arg Arg Met Ala Ile Gly Leu Arg Asn Leu Val Gly Asp Ile 
                245                 250                 255 

ggt caa agt cgt gcg caa ctg gtt tca tcg tcc agc gac ctt tcg gcc      816 
Gly Gln Ser Arg Ala Gln Leu Val Ser Ser Ser Ser Asp Leu Ser Ala 
            260                 265                 270 

atc tgt gct cag gct cag att gat gtc gag tgc cag aag ctt tcg gtc      864 
Ile Cys Ala Gln Ala Gln Ile Asp Val Glu Cys Gln Lys Leu Ser Val 
        275                 280                 285 

gcc cag gtc tct acc gcc gtg aac gag ttg gtt gaa acc gtc cag gca      912 
Ala Gln Val Ser Thr Ala Val Asn Glu Leu Val Glu Thr Val Gln Ala 
    290                 295                 300 

ata gca aaa agc acc gaa gag gca gca aca gtc gcc gtc ttg gcc gat      960 
Ile Ala Lys Ser Thr Glu Glu Ala Ala Thr Val Ala Val Leu Ala Asp 
305                 310                 315                 320 

gaa aag gca cgc ggt ggt gaa agt gtc gtt aac aag gcc gtt gat ttc     1008 
Glu Lys Ala Arg Gly Gly Glu Ser Val Val Asn Lys Ala Val Asp Phe 
                325                 330                 335 

att gag cac ctc tcc gga gat atg gcg gaa ctg gga gac gca atg gag     1056 
Ile Glu His Leu Ser Gly Asp Met Ala Glu Leu Gly Asp Ala Met Glu 
            340                 345                 350 

cgg ctt cag aac gac agt gcg cag atc aat aag gta gta gac gtc att     1104 
Arg Leu Gln Asn Asp Ser Ala Gln Ile Asn Lys Val Val Asp Val Ile 
        355                 360                 365 

aag gct gtg gcg gag cag acc aat ctg cta gcc ctg aat gcg gcg ata     1152 
Lys Ala Val Ala Glu Gln Thr Asn Leu Leu Ala Leu Asn Ala Ala Ile 
    370                 375                 380 

gag gcg gcc cgt gca gga gag cag ggc agg ggc ttt gcg gtc gtg gcg     1200 
Glu Ala Ala Arg Ala Gly Glu Gln Gly Arg Gly Phe Ala Val Val Ala 
385                 390                 395                 400 

gat gag gtt cgt gct ttg gcg atg cgc acc caa caa tcg acc aaa gaa     1248 
Asp Glu Val Arg Ala Leu Ala Met Arg Thr Gln Gln Ser Thr Lys Glu 
                405                 410                 415 

att gag agg cta gtg gtt tca ttg cag cag gga agt gaa gct gcg ggc     1296 
Ile Glu Arg Leu Val Val Ser Leu Gln Gln Gly Ser Glu Ala Ala Gly 
            420                 425                 430 

gag ttg atg cgg cgt ggc aag gtc cgg acg cat gac gtc gtt gga ttg     1344 
Glu Leu Met Arg Arg Gly Lys Val Arg Thr His Asp Val Val Gly Leu 
        435                 440                 445 

gcc cag caa gcc gcg cgc cgc gct act cga aat tac cca gct gtc gcc     1392 
Ala Gln Gln Ala Ala Arg Arg Ala Thr Arg Asn Tyr Pro Ala Val Ala 
    450                 455                 460 

ggc atc caa gcg atg aac tat cag atc gcc gct gga gca gag cag caa     1440 
Gly Ile Gln Ala Met Asn Tyr Gln Ile Ala Ala Gly Ala Glu Gln Gln 
465                 470                 475                 480 

ggg gct gct gtg gtt caa atc aac cag aat atg ctt gaa gtg cat aag     1488 
Gly Ala Ala Val Val Gln Ile Asn Gln Asn Met Leu Glu Val His Lys 
                485                 490                 495 

atg gct gac gag tcc gcc att aaa gcg gga cag acc atg aag tca tcg     1536 
Met Ala Asp Glu Ser Ala Ile Lys Ala Gly Gln Thr Met Lys Ser Ser 
            500                 505                 510 

aag gag ctt gct cac ctc ggc agt gcg cta caa aaa tcc gtt gat cga     1584 
Lys Glu Leu Ala His Leu Gly Ser Ala Leu Gln Lys Ser Val Asp Arg 
        515                 520                 525 

ttc cag ctg tag                                                     1596 
Phe Gln Leu 
    530 

 
           
             34  
             531  
             PRT  
             Pseudomonas sp.  
           
            34 

Met Ile Ser Phe Ala Arg Met Ala Glu Ser Leu Gly Val Gln Ala Lys 
  1               5                  10                  15 

Leu Ala Leu Ala Phe Ala Leu Val Leu Cys Val Gly Leu Ile Val Thr 
             20                  25                  30 

Gly Thr Gly Phe Tyr Ser Val His Thr Leu Ser Gly Leu Val Glu Lys 
         35                  40                  45 

Ser Ala Ile Ala Gly Glu Leu Arg Ala Lys Ile Gln Glu Leu Lys Val 
     50                  55                  60 

Leu Glu Gln Arg Ala Leu Phe Ile Ala Asp Glu Gly Ser Leu Lys Gln 
 65                  70                  75                  80 

Arg Ser Ile Leu Leu Ser Gln Val Ile Ala Glu Val Asn Asp Ala Ile 
                 85                  90                  95 

Asp Ile Phe Asp Phe Gln Arg Gly Arg Ser Glu Leu Leu Lys Phe Ala 
            100                 105                 110 

Ala Ser Ser Arg Glu Ala Ser Tyr Ser Ile Glu Val Gly Ser Asn Ala 
        115                 120                 125 

Ala Ala Asp Lys Leu Gln Ser Gly Glu Pro Ser Asp Ala Leu Met Val 
    130                 135                 140 

Ala Asp Lys Lys Leu Asn Val Glu Tyr Glu Gln Leu Ser Ser Ala Val 
145                 150                 155                 160 

Asn Ala Leu Met Gly His Leu Ile Glu Asp Gln Asn Glu Lys Val Pro 
                165                 170                 175 

Leu Ile Tyr Tyr Met Leu Gly Gly Val Thr Leu Phe Thr Met Leu Met 
            180                 185                 190 

Ser Ala Tyr Ser Val Trp Phe Ile Ser Arg Gln Leu Val Pro Pro Leu 
        195                 200                 205 

Lys Ser Thr Val Gln Leu Ala Glu Arg Ile Ala Ser Gly Asp Leu Ala 
    210                 215                 220 

Asp Val Gly Asp Ser Arg Arg Lys Asp Glu Ile Gly Gln Leu Gln Ser 
225                 230                 235                 240 

Ala Thr Arg Arg Met Ala Ile Gly Leu Arg Asn Leu Val Gly Asp Ile 
                245                 250                 255 

Gly Gln Ser Arg Ala Gln Leu Val Ser Ser Ser Ser Asp Leu Ser Ala 
            260                 265                 270 

Ile Cys Ala Gln Ala Gln Ile Asp Val Glu Cys Gln Lys Leu Ser Val 
        275                 280                 285 

Ala Gln Val Ser Thr Ala Val Asn Glu Leu Val Glu Thr Val Gln Ala 
    290                 295                 300 

Ile Ala Lys Ser Thr Glu Glu Ala Ala Thr Val Ala Val Leu Ala Asp 
305                 310                 315                 320 

Glu Lys Ala Arg Gly Gly Glu Ser Val Val Asn Lys Ala Val Asp Phe 
                325                 330                 335 

Ile Glu His Leu Ser Gly Asp Met Ala Glu Leu Gly Asp Ala Met Glu 
            340                 345                 350 

Arg Leu Gln Asn Asp Ser Ala Gln Ile Asn Lys Val Val Asp Val Ile 
        355                 360                 365 

Lys Ala Val Ala Glu Gln Thr Asn Leu Leu Ala Leu Asn Ala Ala Ile 
    370                 375                 380 

Glu Ala Ala Arg Ala Gly Glu Gln Gly Arg Gly Phe Ala Val Val Ala 
385                 390                 395                 400 

Asp Glu Val Arg Ala Leu Ala Met Arg Thr Gln Gln Ser Thr Lys Glu 
                405                 410                 415 

Ile Glu Arg Leu Val Val Ser Leu Gln Gln Gly Ser Glu Ala Ala Gly 
            420                 425                 430 

Glu Leu Met Arg Arg Gly Lys Val Arg Thr His Asp Val Val Gly Leu 
        435                 440                 445 

Ala Gln Gln Ala Ala Arg Arg Ala Thr Arg Asn Tyr Pro Ala Val Ala 
    450                 455                 460 

Gly Ile Gln Ala Met Asn Tyr Gln Ile Ala Ala Gly Ala Glu Gln Gln 
465                 470                 475                 480 

Gly Ala Ala Val Val Gln Ile Asn Gln Asn Met Leu Glu Val His Lys 
                485                 490                 495 

Met Ala Asp Glu Ser Ala Ile Lys Ala Gly Gln Thr Met Lys Ser Ser 
            500                 505                 510 

Lys Glu Leu Ala His Leu Gly Ser Ala Leu Gln Lys Ser Val Asp Arg 
        515                 520                 525 

Phe Gln Leu 
    530 

 
           
             35  
             411  
             DNA  
             Pseudomonas sp.  
           
            35 

ctagcctaac tgttgcgctt caggctccgc atggatcttg tgcagcagca atagcaattg     60 

ttcacgttcg tcatcactca gcatcgacgt cgcgtcttgg tcgctctgta ccacgatctt    120 

cttcagctct ttgagctgcg tctccccagc tttgctgaga aatatcccat aggaacgctt    180 

gtccggcttg cagcgcacgc gcacagcaag gccgagcttc tcgagcttgt tcagcaaggg    240 

aaccagttgt ggtggttcga ttgcgagcat ccgcgctagg tcagcctgca taagcccagg    300 

gctcgcttcg atgattagaa gtgccgacag ctgcgccggg cgtaggtcat atggcgtcag    360 

ggcttcaatc aggccctgag cgagcttcag ctgtgagccg gcgtaaggca t             411 

 
           
             36  
             136  
             PRT  
             Pseudomonas sp.  
           
            36 

Met Pro Tyr Ala Gly Ser Gln Leu Lys Leu Ala Gln Gly Leu Ile Glu 
  1               5                  10                  15 

Ala Leu Thr Pro Tyr Asp Leu Arg Pro Ala Gln Leu Ser Ala Leu Leu 
             20                  25                  30 

Ile Ile Glu Ala Ser Pro Gly Leu Met Gln Ala Asp Leu Ala Arg Met 
         35                  40                  45 

Leu Ala Ile Glu Pro Pro Gln Leu Val Pro Leu Leu Asn Lys Leu Glu 
     50                  55                  60 

Lys Leu Gly Leu Ala Val Arg Val Arg Cys Lys Pro Asp Lys Arg Ser 
 65                  70                  75                  80 

Tyr Gly Ile Phe Leu Ser Lys Ala Gly Glu Thr Gln Leu Lys Glu Leu 
                 85                  90                  95 

Lys Lys Ile Val Val Gln Ser Asp Gln Asp Ala Thr Ser Met Leu Ser 
            100                 105                 110 

Asp Asp Glu Arg Glu Gln Leu Leu Leu Leu Leu His Lys Ile His Ala 
        115                 120                 125 

Glu Pro Glu Ala Gln Gln Leu Gly 
    130                 135 

 
           
             37  
             1446  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(1443)  
               prodict = ”Coniferylaldehyd-Dehydrogenase“ / 
      gene = ”caldh“ 
             
           
            37 

atg agc att ctt ggt ttg aat ggt gcc ccg gtc gga gct gag cag ctg       48 
Met Ser Ile Leu Gly Leu Asn Gly Ala Pro Val Gly Ala Glu Gln Leu 
  1               5                  10                  15 

ggc tcg gct ctt gat cgc atg aag aag gcg cac ctg gag cag ggg cct       96 
Gly Ser Ala Leu Asp Arg Met Lys Lys Ala His Leu Glu Gln Gly Pro 
             20                  25                  30 

gca aac ttg gag ctg cgt ctg agt agg ctg gat cgt gcg att gca atg      144 
Ala Asn Leu Glu Leu Arg Leu Ser Arg Leu Asp Arg Ala Ile Ala Met 
         35                  40                  45 

ctt ctg gaa aat cgt gaa gca att gcc gac gcg gtt tct gct gac ttt      192 
Leu Leu Glu Asn Arg Glu Ala Ile Ala Asp Ala Val Ser Ala Asp Phe 
     50                  55                  60 

ggc aat cgc agc cgt gag caa aca ctg ctt tgc gac att gct ggc tcg      240 
Gly Asn Arg Ser Arg Glu Gln Thr Leu Leu Cys Asp Ile Ala Gly Ser 
 65                  70                  75                  80 

gtg gca agc ctg aag gat agc cgc gag cac gtg gcc aaa tgg atg gag      288 
Val Ala Ser Leu Lys Asp Ser Arg Glu His Val Ala Lys Trp Met Glu 
                 85                  90                  95 

ccc gaa cat cac aag gcg atg ttt cca ggg gcg gag gca cgc gtt gag      336 
Pro Glu His His Lys Ala Met Phe Pro Gly Ala Glu Ala Arg Val Glu 
            100                 105                 110 

ttt cag ccg ctg ggt gtc gtt ggg gtc att agt ccc tgg aac ttc cct      384 
Phe Gln Pro Leu Gly Val Val Gly Val Ile Ser Pro Trp Asn Phe Pro 
        115                 120                 125 

atc gta ctg gcc ttt ggg ccg ctg gcc ggc ata ttc gca gca ggt aat      432 
Ile Val Leu Ala Phe Gly Pro Leu Ala Gly Ile Phe Ala Ala Gly Asn 
    130                 135                 140 

cgc gcc atg ctc aag ccg tcc gag ctt acc ccg cgg act tct gcc ctg      480 
Arg Ala Met Leu Lys Pro Ser Glu Leu Thr Pro Arg Thr Ser Ala Leu 
145                 150                 155                 160 

ctt gcg gag cta att gct cgt tac ttc gat gaa act gag ctg act aca      528 
Leu Ala Glu Leu Ile Ala Arg Tyr Phe Asp Glu Thr Glu Leu Thr Thr 
                165                 170                 175 

gtg ctg ggc gac gct gaa gtc ggt gcg ctg ttc agt gct cag cct ttc      576 
Val Leu Gly Asp Ala Glu Val Gly Ala Leu Phe Ser Ala Gln Pro Phe 
            180                 185                 190 

gat cat ctg atc ttc acc ggc ggc act gcc gtg gcc aag cac atc atg      624 
Asp His Leu Ile Phe Thr Gly Gly Thr Ala Val Ala Lys His Ile Met 
        195                 200                 205 

cgt gcc gcg gcg gat aac cta gtg ccc gtt acc ctg gaa ttg ggt ggc      672 
Arg Ala Ala Ala Asp Asn Leu Val Pro Val Thr Leu Glu Leu Gly Gly 
    210                 215                 220 

aaa tcg ccg gtg atc gtt tcc cgc agt gca gat atg gcg gac gtt gca      720 
Lys Ser Pro Val Ile Val Ser Arg Ser Ala Asp Met Ala Asp Val Ala 
225                 230                 235                 240 

caa cgg gtg ttg acg gtg aaa acc ttc aat gcc ggg caa atc tgt ctg      768 
Gln Arg Val Leu Thr Val Lys Thr Phe Asn Ala Gly Gln Ile Cys Leu 
                245                 250                 255 

gca ccg gac tat gtg ctg ctg ccg gaa gaa tcg ctg gat agc ttt gtc      816 
Ala Pro Asp Tyr Val Leu Leu Pro Glu Glu Ser Leu Asp Ser Phe Val 
            260                 265                 270 

gcc gag gcg acg cgc ttc gtg gcc gca atg tat ccc tcg ctt cta gat      864 
Ala Glu Ala Thr Arg Phe Val Ala Ala Met Tyr Pro Ser Leu Leu Asp 
        275                 280                 285 

aat ccg gat tac acg tcg atc atc aat gcc cga aat ttc gac cgt ctg      912 
Asn Pro Asp Tyr Thr Ser Ile Ile Asn Ala Arg Asn Phe Asp Arg Leu 
    290                 295                 300 

cat cgc tac ctg act gat gcg cag gca aag gga ggg cgc gtc att gaa      960 
His Arg Tyr Leu Thr Asp Ala Gln Ala Lys Gly Gly Arg Val Ile Glu 
305                 310                 315                 320 

atc aat cct gcg gcc gaa gag ttg ggg gat agt ggt atc agg aag atc     1008 
Ile Asn Pro Ala Ala Glu Glu Leu Gly Asp Ser Gly Ile Arg Lys Ile 
                325                 330                 335 

gcg ccc act ttg atc gtg aat gtg tcg gat gaa atg ctg gtc ttg aac     1056 
Ala Pro Thr Leu Ile Val Asn Val Ser Asp Glu Met Leu Val Leu Asn 
            340                 345                 350 

gag gag atc ttt ggt ccg ctg ctc ccg atc aag act tat cgt gat ttc     1104 
Glu Glu Ile Phe Gly Pro Leu Leu Pro Ile Lys Thr Tyr Arg Asp Phe 
        355                 360                 365 

gac tcg gct atc gac tac gtc aac agc aag cag cga cca ctt gcc tcg     1152 
Asp Ser Ala Ile Asp Tyr Val Asn Ser Lys Gln Arg Pro Leu Ala Ser 
    370                 375                 380 

tac ttc ttc ggc gaa gat gcg gtt gag cgt gag caa gtg ctt aag cgt     1200 
Tyr Phe Phe Gly Glu Asp Ala Val Glu Arg Glu Gln Val Leu Lys Arg 
385                 390                 395                 400 

acg gtt tcg ggc gcc gtg gtc gtg aac gat gtc atg agc cat gtg atg     1248 
Thr Val Ser Gly Ala Val Val Val Asn Asp Val Met Ser His Val Met 
                405                 410                 415 

atg gat acg ctt cca ttt ggt ggt gtg ggg cac tcg ggg atg ggg gca     1296 
Met Asp Thr Leu Pro Phe Gly Gly Val Gly His Ser Gly Met Gly Ala 
            420                 425                 430 

tat cac ggc att tat ggt ttc cga acc ttc agc cat gcc aag cct gtt     1344 
Tyr His Gly Ile Tyr Gly Phe Arg Thr Phe Ser His Ala Lys Pro Val 
        435                 440                 445 

ctc gtg caa agt cct gtg ggt gag tcg aac ttg gcg atg cgc gca ccc     1392 
Leu Val Gln Ser Pro Val Gly Glu Ser Asn Leu Ala Met Arg Ala Pro 
    450                 455                 460 

tac gga gaa gcg atc cac gga ctg ctc tct gtc ctc ctt tca acg gag     1440 
Tyr Gly Glu Ala Ile His Gly Leu Leu Ser Val Leu Leu Ser Thr Glu 
465                 470                 475                 480 

tgt tag                                                             1446 
Cys 

 
           
             38  
             481  
             PRT  
             Pseudomonas sp.  
           
            38 

Met Ser Ile Leu Gly Leu Asn Gly Ala Pro Val Gly Ala Glu Gln Leu 
  1               5                  10                  15 

Gly Ser Ala Leu Asp Arg Met Lys Lys Ala His Leu Glu Gln Gly Pro 
             20                  25                  30 

Ala Asn Leu Glu Leu Arg Leu Ser Arg Leu Asp Arg Ala Ile Ala Met 
         35                  40                  45 

Leu Leu Glu Asn Arg Glu Ala Ile Ala Asp Ala Val Ser Ala Asp Phe 
     50                  55                  60 

Gly Asn Arg Ser Arg Glu Gln Thr Leu Leu Cys Asp Ile Ala Gly Ser 
 65                  70                  75                  80 

Val Ala Ser Leu Lys Asp Ser Arg Glu His Val Ala Lys Trp Met Glu 
                 85                  90                  95 

Pro Glu His His Lys Ala Met Phe Pro Gly Ala Glu Ala Arg Val Glu 
            100                 105                 110 

Phe Gln Pro Leu Gly Val Val Gly Val Ile Ser Pro Trp Asn Phe Pro 
        115                 120                 125 

Ile Val Leu Ala Phe Gly Pro Leu Ala Gly Ile Phe Ala Ala Gly Asn 
    130                 135                 140 

Arg Ala Met Leu Lys Pro Ser Glu Leu Thr Pro Arg Thr Ser Ala Leu 
145                 150                 155                 160 

Leu Ala Glu Leu Ile Ala Arg Tyr Phe Asp Glu Thr Glu Leu Thr Thr 
                165                 170                 175 

Val Leu Gly Asp Ala Glu Val Gly Ala Leu Phe Ser Ala Gln Pro Phe 
            180                 185                 190 

Asp His Leu Ile Phe Thr Gly Gly Thr Ala Val Ala Lys His Ile Met 
        195                 200                 205 

Arg Ala Ala Ala Asp Asn Leu Val Pro Val Thr Leu Glu Leu Gly Gly 
    210                 215                 220 

Lys Ser Pro Val Ile Val Ser Arg Ser Ala Asp Met Ala Asp Val Ala 
225                 230                 235                 240 

Gln Arg Val Leu Thr Val Lys Thr Phe Asn Ala Gly Gln Ile Cys Leu 
                245                 250                 255 

Ala Pro Asp Tyr Val Leu Leu Pro Glu Glu Ser Leu Asp Ser Phe Val 
            260                 265                 270 

Ala Glu Ala Thr Arg Phe Val Ala Ala Met Tyr Pro Ser Leu Leu Asp 
        275                 280                 285 

Asn Pro Asp Tyr Thr Ser Ile Ile Asn Ala Arg Asn Phe Asp Arg Leu 
    290                 295                 300 

His Arg Tyr Leu Thr Asp Ala Gln Ala Lys Gly Gly Arg Val Ile Glu 
305                 310                 315                 320 

Ile Asn Pro Ala Ala Glu Glu Leu Gly Asp Ser Gly Ile Arg Lys Ile 
                325                 330                 335 

Ala Pro Thr Leu Ile Val Asn Val Ser Asp Glu Met Leu Val Leu Asn 
            340                 345                 350 

Glu Glu Ile Phe Gly Pro Leu Leu Pro Ile Lys Thr Tyr Arg Asp Phe 
        355                 360                 365 

Asp Ser Ala Ile Asp Tyr Val Asn Ser Lys Gln Arg Pro Leu Ala Ser 
    370                 375                 380 

Tyr Phe Phe Gly Glu Asp Ala Val Glu Arg Glu Gln Val Leu Lys Arg 
385                 390                 395                 400 

Thr Val Ser Gly Ala Val Val Val Asn Asp Val Met Ser His Val Met 
                405                 410                 415 

Met Asp Thr Leu Pro Phe Gly Gly Val Gly His Ser Gly Met Gly Ala 
            420                 425                 430 

Tyr His Gly Ile Tyr Gly Phe Arg Thr Phe Ser His Ala Lys Pro Val 
        435                 440                 445 

Leu Val Gln Ser Pro Val Gly Glu Ser Asn Leu Ala Met Arg Ala Pro 
    450                 455                 460 

Tyr Gly Glu Ala Ile His Gly Leu Leu Ser Val Leu Leu Ser Thr Glu 
465                 470                 475                 480 

Cys 

 
           
             39  
             1827  
             DNA  
             Pseudomonas sp.  
           
            39 

ctatttgtct agtggtcggc gcgaaattcg ataagaaagc tgggcgcgag tgaggccgag     60 

ccggcgggca gcttccgaga cattgccttt cacctggccc agagcatggc taatcatcgc    120 

gtcctccact tcttgcagcg tcatcgcgct caggtccttt gagtcaagcg gcgagtcgat    180 

tgtgctggtc ggtttggaga aggaagtact tgggctgcca gtttcctgtg gctgattatc    240 

ttgagcggtg gccaggatgc cgctggcccc aatggagaac atcggttgag tcagtcgttc    300 

accgctagtg aagaggtggc tcacgtcaat ggctccatcc tccggagcgc tgatgactcc    360 

gcgctccacc aaattttgaa gctcccggat gtttcctgga aagtcgtagc caagcagggc    420 

attggctgca cgtggagtga atccgctgac cacccggcta tgacgctgat tgaagcggtg    480 

caggaaatag gtcatcagga ggggaatgtc ttccttcctc tctcgaagcg gcgggaggtg    540 

gatcgggtaa acattgaggc ggaaaaaaag gtcctcgcgg aactcgccgc gctggacgcc    600 

tgcgcgaaga tcgacattgg ttgcggctac cacacggacg tcaaccttga gtgtcctgct    660 

tccgccaacc cgttcgacct ccgactcttg cagggcgcga agtaacttcc cttgggccac    720 

gaggcttagc gtccctatct cgtcaaggaa tagtgtgccg cccgaagcgc gctcgaaccg    780 

tcctgctcga gattgggtgg cgccggtaaa cgccccccgt tcgacgccga acaactcgga    840 

ctccatcagg gtttcgggaa tacgtgcgca attgaccgca acaaacgggc cgtcgtgtct    900 

ggggctgatg cggtgaagca tgcgggcgaa catctccttg cccacacctg attcacccgt    960 

aaacagtacc gtcgcctccg tgggtgctac gcgcttcagc atgtggcagg cagcattgaa   1020 

tgccgaggaa attcccacca tgtcgtgttc cgatgcagtg cttgagtctg cggcggagtg   1080 

atggggagtg ttcctttgtc cctgctgcgt tcttcgtctc tgcggcgtgc ttggttgccg   1140 

acaaatggtt gcgctaagcg ccgccaagtc ctcttcggcg tcttcccatt cttccgctgg   1200 

cttgccgatc atgcggcaga tctgcgaacc cgtggagcgg cattccacct ctcggtaaag   1260 

gatgaggcga ccaaccagcg cggacgtata gccaatggca taacccgtct gcgtccagca   1320 

cgcgggctcg gtgccgatgc cgtagtgcgc aatatgttca tcatcttcgc tcgaatggtg   1380 

ccagaggaat tcgccgtagt aggtccccaa atccatgtcg aagtcgaagt ggatcggctc   1440 

cacgcgtact gcgccttcca gagagtgcaa gttcgggccg gcggcaaata gggagagcgg   1500 

atcggcgttg ctgaagcgct ccttcagaag ggcggcatct ttggcgccgc agtggtaacc   1560 

ggttcgcagc atgattccgc gggcgcgggc gaagcccacg ctttcaatta attcgcgtcg   1620 

caatgcaccc agtccgctgc tgtggaggag cagcattcgc gcgccgttca accagatgcg   1680 

tccatcgcca gggctgaaaa ggagggattc agtgaggtca tgaagggagg ggacggcgcc   1740 

tggctccaat tgctcgatgg cgccgcgatt gagtgtcttg ggcgcggtct tggagagttc   1800 

ggctagggag ataaatttgc tggccat                                       1827 

 
           
             40  
             608  
             PRT  
             Pseudomonas sp.  
           
            40 

Met Ala Ser Lys Phe Ile Ser Leu Ala Glu Leu Ser Lys Thr Ala Pro 
  1               5                  10                  15 

Lys Thr Leu Asn Arg Gly Ala Ile Glu Gln Leu Glu Pro Gly Ala Val 
             20                  25                  30 

Pro Ser Leu His Asp Leu Thr Glu Ser Leu Leu Phe Ser Pro Gly Asp 
         35                  40                  45 

Gly Arg Ile Trp Leu Asn Gly Ala Arg Met Leu Leu Leu His Ser Ser 
     50                  55                  60 

Gly Leu Gly Ala Leu Arg Arg Glu Leu Ile Glu Ser Val Gly Phe Ala 
 65                  70                  75                  80 

Arg Ala Arg Gly Ile Met Leu Arg Thr Gly Tyr His Cys Gly Ala Lys 
                 85                  90                  95 

Asp Ala Ala Leu Leu Lys Glu Arg Phe Ser Asn Ala Asp Pro Leu Ser 
            100                 105                 110 

Leu Phe Ala Ala Gly Pro Asn Leu His Ser Leu Glu Gly Ala Val Arg 
        115                 120                 125 

Val Glu Pro Ile His Phe Asp Phe Asp Met Asp Leu Gly Thr Tyr Tyr 
    130                 135                 140 

Gly Glu Phe Leu Trp His His Ser Ser Glu Asp Asp Glu His Ile Ala 
145                 150                 155                 160 

His Tyr Gly Ile Gly Thr Glu Pro Ala Cys Trp Thr Gln Thr Gly Tyr 
                165                 170                 175 

Ala Ile Gly Tyr Thr Ser Ala Leu Val Gly Arg Leu Ile Leu Tyr Arg 
            180                 185                 190 

Glu Val Glu Cys Arg Ser Thr Gly Ser Gln Ile Cys Arg Met Ile Gly 
        195                 200                 205 

Lys Pro Ala Glu Glu Trp Glu Asp Ala Glu Glu Asp Leu Ala Ala Leu 
    210                 215                 220 

Ser Ala Thr Ile Cys Arg Gln Pro Ser Thr Pro Gln Arg Arg Arg Thr 
225                 230                 235                 240 

Gln Gln Gly Gln Arg Asn Thr Pro His His Ser Ala Ala Asp Ser Ser 
                245                 250                 255 

Thr Ala Ser Glu His Asp Met Val Gly Ile Ser Ser Ala Phe Asn Ala 
            260                 265                 270 

Ala Cys His Met Leu Lys Arg Val Ala Pro Thr Glu Ala Thr Val Leu 
        275                 280                 285 

Phe Thr Gly Glu Ser Gly Val Gly Lys Glu Met Phe Ala Arg Met Leu 
    290                 295                 300 

His Arg Ile Ser Pro Arg His Asp Gly Pro Phe Val Ala Val Asn Cys 
305                 310                 315                 320 

Ala Arg Ile Pro Glu Thr Leu Met Glu Ser Glu Leu Phe Gly Val Glu 
                325                 330                 335 

Arg Gly Ala Phe Thr Gly Ala Thr Gln Ser Arg Ala Gly Arg Phe Glu 
            340                 345                 350 

Arg Ala Ser Gly Gly Thr Leu Phe Leu Asp Glu Ile Gly Thr Leu Ser 
        355                 360                 365 

Leu Val Ala Gln Gly Lys Leu Leu Arg Ala Leu Gln Glu Ser Glu Val 
    370                 375                 380 

Glu Arg Val Gly Gly Ser Arg Thr Leu Lys Val Asp Val Arg Val Val 
385                 390                 395                 400 

Ala Ala Thr Asn Val Asp Leu Arg Ala Gly Val Gln Arg Gly Glu Phe 
                405                 410                 415 

Arg Glu Asp Leu Phe Phe Arg Leu Asn Val Tyr Pro Ile His Leu Pro 
            420                 425                 430 

Pro Leu Arg Glu Arg Lys Glu Asp Ile Pro Leu Leu Met Thr Tyr Phe 
        435                 440                 445 

Leu His Arg Phe Asn Gln Arg His Ser Arg Val Val Ser Gly Phe Thr 
    450                 455                 460 

Pro Arg Ala Ala Asn Ala Leu Leu Gly Tyr Asp Phe Pro Gly Asn Ile 
465                 470                 475                 480 

Arg Glu Leu Gln Asn Leu Val Glu Arg Gly Val Ile Ser Ala Pro Glu 
                485                 490                 495 

Asp Gly Ala Ile Asp Val Ser His Leu Phe Thr Ser Gly Glu Arg Leu 
            500                 505                 510 

Thr Gln Pro Met Phe Ser Ile Gly Ala Ser Gly Ile Leu Ala Thr Ala 
        515                 520                 525 

Gln Asp Asn Gln Pro Gln Glu Thr Gly Ser Pro Ser Thr Ser Phe Ser 
    530                 535                 540 

Lys Pro Thr Ser Thr Ile Asp Ser Pro Leu Asp Ser Lys Asp Leu Ser 
545                 550                 555                 560 

Ala Met Thr Leu Gln Glu Val Glu Asp Ala Met Ile Ser His Ala Leu 
                565                 570                 575 

Gly Gln Val Lys Gly Asn Val Ser Glu Ala Ala Arg Arg Leu Gly Leu 
            580                 585                 590 

Thr Arg Ala Gln Leu Ser Tyr Arg Ile Ser Arg Arg Pro Leu Asp Lys 
        595                 600                 605 

 
           
             41  
             768  
             DNA  
             Pseudomonas sp.  
             
               CDS  
               (1)..(765)  
               product = ”Coniferylalkohol-Dehydrogenase“ / 
      gene = ”cadh“ 
             
           
            41 

atg caa ctg acc aac aag aaa atc gtc gtc acc gga gtg tcc tcc ggt       48 
Met Gln Leu Thr Asn Lys Lys Ile Val Val Thr Gly Val Ser Ser Gly 
  1               5                  10                  15 

atc ggt gcc gaa act gcc cgc gtt ctg cgc tct cac ggc gcc aca gtg       96 
Ile Gly Ala Glu Thr Ala Arg Val Leu Arg Ser His Gly Ala Thr Val 
             20                  25                  30 

att ggc gta gat cgc aac atg ccg agc ctg act ctg gat gct ttc gtt      144 
Ile Gly Val Asp Arg Asn Met Pro Ser Leu Thr Leu Asp Ala Phe Val 
         35                  40                  45 

cag gct gac ctg agc cat cct gaa ggc atc gat aag gcc atc tct cag      192 
Gln Ala Asp Leu Ser His Pro Glu Gly Ile Asp Lys Ala Ile Ser Gln 
     50                  55                  60 

ctg ccg gag aaa att gac gga ctc tgc aat atc gcc ggg gtg ccc ggc      240 
Leu Pro Glu Lys Ile Asp Gly Leu Cys Asn Ile Ala Gly Val Pro Gly 
 65                  70                  75                  80 

act gcc gat cct cag ctc gtc gca aac gtg aac tac ctg ggt cta aag      288 
Thr Ala Asp Pro Gln Leu Val Ala Asn Val Asn Tyr Leu Gly Leu Lys 
                 85                  90                  95 

tat ctg acc gag gca gtc ctg tcg cgc att caa ccc ggt ggt tcg att      336 
Tyr Leu Thr Glu Ala Val Leu Ser Arg Ile Gln Pro Gly Gly Ser Ile 
            100                 105                 110 

gtc aac gtg tcc tct gtg ctt ggc gcc gag tgg ccg gcc cgc ctt cag      384 
Val Asn Val Ser Ser Val Leu Gly Ala Glu Trp Pro Ala Arg Leu Gln 
        115                 120                 125 

ttg cat aag gag ctg ggg agt gtt gtt gga ttc tcc gaa ggc cag gca      432 
Leu His Lys Glu Leu Gly Ser Val Val Gly Phe Ser Glu Gly Gln Ala 
    130                 135                 140 

tgg ctt aag cag aat cca gtg gcc ccc gaa ttc tgc tac cag tat ttc      480 
Trp Leu Lys Gln Asn Pro Val Ala Pro Glu Phe Cys Tyr Gln Tyr Phe 
145                 150                 155                 160 

aaa gaa gca ctg atc gtt tgg tct caa gtt cag gcg cag gaa tgg ttc      528 
Lys Glu Ala Leu Ile Val Trp Ser Gln Val Gln Ala Gln Glu Trp Phe 
                165                 170                 175 

atg agg acg tct gta cgc atg aac tgc atc gcc ccc ggc cct gta ttc      576 
Met Arg Thr Ser Val Arg Met Asn Cys Ile Ala Pro Gly Pro Val Phe 
            180                 185                 190 

act ccc att ctc aat gag ttc gtc acc atg ctg ggt caa gag cgg act      624 
Thr Pro Ile Leu Asn Glu Phe Val Thr Met Leu Gly Gln Glu Arg Thr 
        195                 200                 205 

cag gcg gac gct cat cgt att aag cgc cca gca tat gcc gat gaa gtg      672 
Gln Ala Asp Ala His Arg Ile Lys Arg Pro Ala Tyr Ala Asp Glu Val 
    210                 215                 220 

gcc gcg gtg att gca ttc atg tgt gct gag gag tca cgt tgg atc aac      720 
Ala Ala Val Ile Ala Phe Met Cys Ala Glu Glu Ser Arg Trp Ile Asn 
225                 230                 235                 240 

ggc ata aat att cca gtg gac gga ggt ttg gca tcg acc tac gtg taa      768 
Gly Ile Asn Ile Pro Val Asp Gly Gly Leu Ala Ser Thr Tyr Val 
                245                 250                 255 

 
           
             42  
             255  
             PRT  
             Pseudomonas sp.  
           
            42 

Met Gln Leu Thr Asn Lys Lys Ile Val Val Thr Gly Val Ser Ser Gly 
  1               5                  10                  15 

Ile Gly Ala Glu Thr Ala Arg Val Leu Arg Ser His Gly Ala Thr Val 
             20                  25                  30 

Ile Gly Val Asp Arg Asn Met Pro Ser Leu Thr Leu Asp Ala Phe Val 
         35                  40                  45 

Gln Ala Asp Leu Ser His Pro Glu Gly Ile Asp Lys Ala Ile Ser Gln 
     50                  55                  60 

Leu Pro Glu Lys Ile Asp Gly Leu Cys Asn Ile Ala Gly Val Pro Gly 
 65                  70                  75                  80 

Thr Ala Asp Pro Gln Leu Val Ala Asn Val Asn Tyr Leu Gly Leu Lys 
                 85                  90                  95 

Tyr Leu Thr Glu Ala Val Leu Ser Arg Ile Gln Pro Gly Gly Ser Ile 
            100                 105                 110 

Val Asn Val Ser Ser Val Leu Gly Ala Glu Trp Pro Ala Arg Leu Gln 
        115                 120                 125 

Leu His Lys Glu Leu Gly Ser Val Val Gly Phe Ser Glu Gly Gln Ala 
    130                 135                 140 

Trp Leu Lys Gln Asn Pro Val Ala Pro Glu Phe Cys Tyr Gln Tyr Phe 
145                 150                 155                 160 

Lys Glu Ala Leu Ile Val Trp Ser Gln Val Gln Ala Gln Glu Trp Phe 
                165                 170                 175 

Met Arg Thr Ser Val Arg Met Asn Cys Ile Ala Pro Gly Pro Val Phe 
            180                 185                 190 

Thr Pro Ile Leu Asn Glu Phe Val Thr Met Leu Gly Gln Glu Arg Thr 
        195                 200                 205 

Gln Ala Asp Ala His Arg Ile Lys Arg Pro Ala Tyr Ala Asp Glu Val 
    210                 215                 220 

Ala Ala Val Ile Ala Phe Met Cys Ala Glu Glu Ser Arg Trp Ile Asn 
225                 230                 235                 240 

Gly Ile Asn Ile Pro Val Asp Gly Gly Leu Ala Ser Thr Tyr Val 
                245                 250                 255 

 
           
             43  
             26  
             DNA  
             Pseudomonas sp.  
           
            43 

atgcarctba cbaayaaraa ratygt                                          26 

 
           
             44  
             20  
             PRT  
             Pseudomonas sp.  
             
               UNSURE  
               (12)  
             
             
               UNSURE  
               (13)..(19)  
             
           
            44 

Met Gln Leu Thr Asn Lys Lys Ile Val Val Val Xaa Val Xaa Xaa Xaa 
  1               5                  10                  15 

Xaa Xaa Xaa Xaa 
             20 

 
           
             45  
             20  
             PRT  
             Pseudomonas sp.  
             
               UNSURE  
               (20)  
             
           
            45 

Ser Ile Leu Gly Leu Asn Gly Ala Pro Val Gly Ala Glu Gln Leu Gly 
  1               5                  10                  15 

Ser Ala Leu Xaa 
             20