Patent Publication Number: US-2016244808-A1

Title: Container, liquid storing member, cartridge set, and method of manufacturing liquid storing member

Description:
BACKGROUND 
     1. Technical Field 
     The present invention relates to a container which stores a liquid inside thereof, a liquid storing member in which a liquid is sealed and stored, a cartridge set which enables plural liquid storing members to be bound, and a method of manufacturing the liquid storing member. 
     2. Related Art 
     In the field of biochemistry, a technology of a polymerase chain reaction (PCR) has been established. Recently, accuracy in amplification or detection sensitivity in a PCR method has been improved such that it is possible to amplify and detect/analyze an infinitesimal trace of a specimen (DNA or the like). The PCR is a technology in which a thermal cycle is performed on nucleic acids (target nucleic acids) as an amplification target and a solution (reaction solution) including a reagent, and thereby the target nucleic acids are amplified. In general, as the thermal cycle of PCR, a technology, in which the thermal cycle is performed at two-level or three-level temperatures, is employed. 
     Meanwhile, diagnosis of an infection such as influenza in a field of medical care is mainly performed by using a simple test kit such as an immunochromatography kit in the present circumstances. However, in such a simple test, the test may be performed with insufficient accuracy and it is desirable that the PCR, which can be expected to perform the test with higher accuracy, is applied to diagnosis of an infection. 
     In recent years, as a device used in the PCR method or the like, a device, in which a water-based liquid layer and a water-insoluble gel layer are alternately stacked in a capillary (in a cartridge), magnetic particles, to which nucleic acids are attached, pass through the layers, and thereby purification of the nucleic acid is performed (see International Publication No. 2012/086243). Also, International Publication No. 2012/086243 discloses that a nucleic acid amplification reaction solution is accommodated in the lowermost layer of the cartridge and amplification of a target nucleic acid in the nucleic acid amplification reaction solution is performed. 
     However, the device described above is configured to include a container which is integrally formed from a reagent supply section to the nucleic acid amplification reaction solution collecting section. For example, in a case where such a device is kept for a long period of time, a component contained in a cleaning liquid, an eluate, or the like, may be dispersed through oil, which results in contamination, and the PCR may be inhibited. In addition, when outside air infiltrates into a liquid in the device and bubbles are formed, a purification process of the nucleic acids may be inhibited. 
     SUMMARY 
     An advantage of some aspects of the invention is to provide a container in which it is possible to prevent bubbles from being mixed to a liquid when the liquid is stored in the container. Another advantage of some aspects of the invention is to provide a liquid storing member and a method of manufacturing the liquid storing member in which the liquid is sealed and stored while preventing bubbles from being mixed in the liquid. Still another advantage of some aspects of the invention is to provide a cartridge set which enables plural liquid storing members to be bound. 
     Application Example 1 
     A container according to this application example of the invention has an opening and a liquid is sealed and stored therein by sealing the opening, and the container includes: an annular wall section having an annular wall surface formed around the opening; and an attachment surface which is formed on the inner side of the annular wall section and to which a film sealing the opening is attached. The annular wall section has a height higher than the attachment surface. 
     According to the container related to this application example, since it is possible to attach the film to the attachment surface in a liquid, it is possible to prevent bubbles from being mixed into the liquid. 
     Application Example 2 
     In the container according to the application example of the invention, the attachment surface may be an annular step section formed on a wall surface on the inner side of the annular wall section. 
     According to the container related to this application example, it is possible to position the film on the wall surface on the inner side and to attach the film to the attachment surface. 
     Application Example 3 
     In the container according to the application example of the invention, the opening, the annular wall section, the film, and the attachment surface are a first opening, a first annular wall section, a first film, and a first attachment surface, respectively, the container may further include: a second opening different from the first opening; a second annular wall section having an annular wall surface formed around the second opening; and a second annular attachment surface which is formed on the inner side of the second annular wall section and to which a second film sealing the second opening is attached, and the second annular wall section may have a height higher than the second attachment surface. 
     According to the container related to this application example, since it is possible to attach the film to the attachment surface in a liquid on both two openings, it is possible to prevent bubbles from being mixed into the liquid. 
     Application Example 4 
     In the container according to the application example of the invention, the container may have a longitudinal direction, the first opening may be formed in one end portion of the container, and the second opening may be formed in the other end portion of the container. 
     According to the container related to this application example, this can be applicable to both end openings of the container in the longitudinal direction. 
     Application Example 5 
     A liquid storing member according to this application example of the invention includes the container to which the film is attached to the attachment surface thereof and in which a liquid is sealed and stored. 
     According to the liquid storing member related to this application example, it is possible to seal and store the liquid in a state in which bubbles are less likely to be mixed. 
     Application Example 6 
     A liquid storing member according to this application example of the invention includes the container to which the first film is attached on the first attachment surface thereof and the second film is attached on the second attachment surface thereof and in which a liquid is sealed and stored between the first film and the second film. 
     According to the liquid storing member related to this application example, it is possible to seal and store the liquid in a state in which bubbles are less likely to be mixed. 
     Application Example 7 
     A cartridge set according to this application example of the invention includes: the liquid storing member; and another liquid storing member which is bound to the liquid storing member. A liquid is sealed and stored in a first flow path of the liquid storing member and the liquid storing member further includes an insertion section which is inserted into the another liquid storing member. An inside surface of the insertion section forms a part of the first flow path. An opening end of the insertion section is formed at a position lower than the attachment surface. The another liquid storing member has a second flow path that stores another liquid inside, a third annular wall section having an annular wall surface on one end portion side, a third annular attachment surface that is formed on the inner side of the third annular wall section and to which a third film is attached. The third annular wall section has a height higher than the third attachment surface. The third film is attached to the third attachment surface and the another liquid is sealed and stored in the second flow path. 
     According to the cartridge set related to the application example, it is possible to bind two liquid storing members in which the liquids are sealed and stored in a state in which bubbles are less likely to be mixed. In addition, in this case, it is possible to assemble the cartridge that stores the liquid in a state in which bubbles are less likely to be mixed in a flow path which is formed to communicate with two flow paths. 
     Application Example 8 
     A method of manufacturing a liquid storing member according to this application example of the invention includes: injecting a liquid into the container; filling the container with liquid at a level higher than the attachment surface; and attaching the film to the attachment surface in the liquid to seal and store the liquid. 
     According to the method of manufacturing a liquid storing member related to this application example, the film is attached in the liquid, and thereby it is possible to prevent bubbles from being mixed into the liquid sealed and stored. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The invention will be described with reference to the accompanying drawings, wherein like numbers reference like elements. 
         FIG. 1  is a front view of a container assembly according to an embodiment. 
         FIG. 2  is a side view of the container assembly according to the embodiment. 
         FIG. 3  is a plan view of the container assembly according to the embodiment. 
         FIG. 4  is a perspective view of the container assembly according to the embodiment. 
         FIG. 5  is a sectional view of the container assembly according to the embodiment, which is taken along line A-A in  FIG. 3 . 
         FIG. 6  is a sectional view of the container assembly according to the embodiment, which is taken along line C-C in  FIG. 3 . 
         FIGS. 7A and 7B  are views schematically illustrating an operation of the container assembly according to the embodiment. 
         FIGS. 8A and 8B  are views schematically illustrating an operation of the container assembly according to the embodiment. 
         FIG. 9  is a diagram of a schematic configuration of a PCR device. 
         FIG. 10  is a block diagram of the PCR device. 
         FIG. 11  is a perspective view of an elution container according to an embodiment. 
         FIG. 12  is a sectional view of a part of the elution container on a first end portion, which is taken along line B-B. 
         FIG. 13  is a sectional view of a part of the elution container on a second end portion, which is taken along line B-B. 
         FIG. 14  is a perspective view of a reaction container according to an embodiment. 
         FIG. 15  is a sectional view of apart of the reaction container on a third end portion side, which is taken along line B-B. 
         FIG. 16  is a sectional view of a cartridge set according to an embodiment, which is taken along line B-B. 
         FIG. 17  is a sectional view of a part of a nuclei acid amplifying reaction cartridge, which is taken along line B-B. 
     
    
    
     DESCRIPTION OF EXEMPLARY EMBODIMENTS 
     Hereinafter, preferred embodiments of the invention will be described in detail with reference to the drawings. Further, the embodiments to be described below do not inappropriately limit the content of the invention described in the appended claims. In addition, every configuration to be described below is not essential requirements of the invention. 
     A container according to the present embodiment has an opening and a liquid is sealed and stored therein by sealing the opening. The container includes: an annular wall section having an annular wall surface formed around the opening; and an annular attachment surface which is formed on the inner side of the annular wall section and to which a film sealing the opening is attached. The annular wall section has a height higher than the attachment surface. 
     In a liquid storing member according to the present embodiment, a liquid is sealed and stored with the film attached to the attachment surface of the container. 
     A cartridge set according to the present embodiment includes: the liquid storing member; and another liquid storing member which is bound to the liquid storing member. A liquid is sealed and stored in a first flow path of the liquid storing member and the liquid storing member further includes an insertion section which is inserted into the another liquid storing member. An inside surface of the insertion section forms a part of the first flow path. An opening end of the insertion section is formed at a position lower than the attachment surface. The another liquid storing member has a second flow path that stores another liquid inside, a third annular wall section having an annular wall surface on one end portion side, a third annular attachment surface that is formed on the inner side of the third annular wall section and to which a third film is attached. The third annular wall section has a height higher than the third attachment surface. The third film is attached to the third attachment surface and the another liquid is sealed and stored in the second flow path. 
     A method of manufacturing a liquid storing member according to the present embodiment includes: injecting a liquid into the container; filling the container with the liquid at a level higher than the attachment surface; and attaching the film to the attachment surface in the liquid to seal and store the liquid. 
     For the cartridge set according to the invention, a set for assembling cartridges, in which a nucleic acid amplification reaction is performed, is described. In other words, when a nucleic acid amplification reaction cartridge set according to the invention is assembled, it is possible to obtain a cartridge for performing a nucleic acid amplification reaction. Hereinafter, the cartridge (container assembly) is, first, described, and then the container, the liquid storing member, a method of manufacturing the liquid storing member, and the nucleic acid amplification reaction cartridge set will be described. 
     1. Outline of Container Assembly 
     First, an outline of a container assembly  1  according to the present embodiment is described with respect to  FIG. 1  to  FIG. 4 .  FIG. 1  is a front view of the container assembly  1  according to the embodiment (hereinafter may be referred to as a cartridge).  FIG. 2  is a side view of the container assembly  1  according to the embodiment.  FIG. 3  is a plan view of the container assembly  1  according to the embodiment.  FIG. 4  is a perspective view of the container assembly  1  according to the embodiment. Further, a state of the container assembly  1  in  FIG. 1  to  FIG. 3  is described as an upright state. 
     The container assembly  1  includes an adsorption container  100 , a cleaning container  200 , an elution container  300 , and a reaction container  400 . The container assembly  1  is a container forming a flow path (not illustrated) through which communication from the adsorption container  100  to reaction container  400  is performed. One end of the flow path of the container assembly  1  is closed by a cap  110  and the other end thereof is closed by a bottom  402 . 
     In the container assembly  1 , preprocessing of combining nucleic acids with a magnetic bead (not illustrated) in the adsorption container  100 , purifying the nucleic acids with the magnetic bead moving in the cleaning container  200 , and eluting the nucleic acids in an eluate droplet (not illustrated) in the elution container  300 , and thermal cycle processing of a polymerase reaction to the eluate droplet containing the nucleic acids in the reaction container  400  are performed. 
     A material for the container assembly  1  is not particularly limited; however, it is possible to use, for example, glass, a polymer, metal, or the like. It is more preferable that a material such as glass or the polymer, which has transparency in the visible light, is selected as the material of the container assembly  1 , because it is possible to observe the inside (cavity) of the container assembly  1  from the outside thereof. In addition, it is preferable that a material, which transmits a magnetic force, or a nonmagnetic material is selected as the material of the container assembly  1 , because it is easy to pass the magnetic bead (not illustrated) through the container assembly  1  by applying a magnetic force from the outside of the container assembly  1 . For the material of the container assembly  1 , it is possible to use a polypropylene resin. 
     The adsorption container  100  includes a cylindrical syringe section  120  which accommodates an adsorption solution (not illustrated) inside, a plunger section  130  which a movable plunger inserted into the inside of the syringe section  120 , and a cap  110  fixed to one end portion of the plunger section  130 . In the adsorption container  100 , the cap  110  moves to the syringe section  120  such that the plunger section  130  slides on the inner surface of the syringe section  120 , and it is possible to extrude the adsorption solution (not illustrated), which is accommodated in the syringe section  120 , to the cleaning container  200 . Further, the adsorption solution will be described below. 
     The cleaning container  200  is obtained by binding and assembling first to third cleaning containers  210 ,  220  and  230 . The first to third cleaning containers  210 ,  220  and  230  have one or more cleaning solution layers partitioned by an oil layer (not illustrated) inside. Also the first to third cleaning containers  210 ,  220  and  230  are bound, and thereby the cleaning container  200  has a plurality of cleaning solution layers partitioned by a plurality of oil layers (not illustrated) inside. In the cleaning container  200  of the present embodiment, an example, in which three cleaning containers of the first to third cleaning containers  210 ,  220  and  230  are used, is described; but the number of cleaning containers is not limited thereto, but the number of cleaning containers is appropriately increased or decreased. The cleaning solution will be described below. 
     The elution container  300  is bound to the third cleaning container  230  of the cleaning container  200  and the eluate is accommodated inside in a state of maintaining a plug shape. Here, the “plug” means a liquid in a case where a specific liquid occupies a zone in the flow path. More specifically, the plug of the specific liquid indicates that only the specific liquid substantially occupies the inside to have a column shape and represents a state in which a certain space inside the flow path is demarcated by the plug. Here, the expression, substantially, indicates that a trace (for example, a thin film shape) of other substances (liquid or the like) may exist around the plug, that is, on the inside wall of the flow path. Further, the eluate will be described below. 
     A nucleic acid purifying device  5  includes the adsorption container  100 , the cleaning container  200 , and the elution container  300 . 
     The reaction container  400  is a container which is bound to the elution container  300  and receives a liquid extruded from the elution container  300  and a container that accommodates an eluate droplet containing a specimen during the thermal cycle processing. In addition, the reaction container  400  accommodates a reagent (not illustrated). Further, the reagent will be described below. 
     2. Detailed Structure of Container Assembly 
     Next, a detailed structure of the container assembly  1  will be described with reference to  FIG. 5  and  FIG. 6 .  FIG. 5  is a sectional view of the container assembly  1  according to the embodiment, which is taken along line A-A in  FIG. 3 .  FIG. 6  is a sectional view of the container assembly  1  according to the embodiment, which is taken along line C-C in  FIG. 3 . Further, actually, the container assembly  1  is assembled in a state of being filled with content such as the cleaning solution; however, in order to describe the structure of the container assembly  1 , depiction of the content is omitted in  FIG. 5  and  FIG. 6 . 
     2-1. Adsorption Container 
     In the adsorption container  100 , the plunger section  130  is inserted from one opening end portion of the syringe section  120  and the cap  110  is inserted into an opening end portion of the plunger section  130 . The cap  110  has a vent section  112  at the center thereof and it is possible to suppress a change in an internal pressure of the plunger section  130  by the vent section  112  when the plunger section  130  is operated. 
     The plunger section  130  is a substantially cylindrical plunger which slides on the inner circumferential surface of the syringe section  120  and has the opening end portion into which the cap  110  is inserted, a rod-shaped section  132  which extends from the bottom facing the opening end portion, in the longitudinal direction of the syringe section  120 , and a distal end portion  134  which is the distal end of the rod-shaped section  132 . The rod-shaped section  132  protrudes from the center of the bottom of the plunger section  130 , and a through-hole is formed on the periphery of the rod-shaped section  132  and communicates with the plunger section  130  and the syringe section  120 . 
     The syringe section  120  constitutes a part of a flow path  2  of the container assembly  1  and has a large-diameter section which accommodates the plunger section  130 , a small-diameter section smaller in size than the large-diameter section, a diameter-reduction section at which the inner diameter is reduced from the large-diameter section to the small-diameter section, an adsorption inserting section  122  on the distal end of the small-diameter section, and a cylindrical adsorption covering section  126  which covers the periphery of the adsorption inserting section  122 . The large-diameter section, the small-diameter section, and the adsorption inserting section  122 , as a part of the flow path  2  of the container assembly  1  have substantially a cylindrical shape. 
     At the time of being provided to an operator, the distal end portion  134  of the plunger section  130  seals the small-diameter section of the syringe section  120 , the large-diameter section and the diameter-reduction section are divided from the small-diameter section, and thus two zones are formed. 
     The adsorption inserting section  122  of the syringe section  120  is inserted and fitted into a first reception section  214  which is one end opening portion of the first cleaning container  210  in the cleaning container  200 , and thereby the syringe section  120  and the first cleaning container  210  are bound. The outer circumferential surface of the adsorption inserting section  122  and the inner circumferential surface of the first reception section  214  are brought into close contact with each other and the liquid as the content is prevented from being leaked to the outside. 
     2-2. Cleaning Container 
     The cleaning container  200  constitutes apart of the flow path  2  of the container assembly  1  and is an assembly of the first to third cleaning containers  210 ,  220  and  230 . Since the first to third cleaning containers  210 ,  220  and  230  have the same fundamental structure, the structure of the first cleaning container  210  is described and description of the second and third cleaning containers  220  and  230  is omitted. 
     The first cleaning container  210  has substantially a cylindrical shape extending in the longitudinal direction of the container assembly  1 , and includes a first insertion section  212  formed on one opening end portion, the first reception section  214  formed on the other opening end portion, and a cylindrical first covering section  216  which covers the periphery of the first insertion section  212 . 
     The outer diameter of the first insertion section  212  is substantially equal to the inner diameter of the second reception section  224 . In addition, the inner diameter of the first reception section  214  is substantially equal to the outer diameter of the adsorption inserting section  122 . 
     The first insertion section  212  of the first cleaning container  210  is inserted and fitted into the second reception section  224  of the second cleaning container  220 , and thereby the outer circumference of the first insertion section  212  and the inner circumference of the second reception section  224  are brought into close contact and sealed with each other, and the first cleaning container  210  and the second cleaning container  220  are bound. Similarly, the first to third cleaning containers  210 ,  220  and  230  are interconnected and the cleaning container  200  is formed. Here, “sealing” means blocking such that at least a liquid or a gas accommodated in the container or the like does not leak to the outside and may include blocking a liquid or a gas from infiltrating into the inside from the outside. 
     2-3. Elution Container 
     The elution container  300  has substantially a cylindrical shape extending in the longitudinal direction of the container assembly  1  and constitutes a part of the flow path  2  of the container assembly  1 . The elution container  300  has an elution inserting section  302  formed on one opening end portion and an elution receiving section  304  formed on the other opening end portion. 
     The inner diameter of the elution receiving section  304  is substantially equal to the outer diameter of a third insertion section  232  of the third cleaning container  230 . The third insertion section  232  is inserted and fitted into the elution receiving section  304 , and thereby the outer circumference of the third insertion section  232  and the inner circumference of the elution receiving section  304  are brought into close contact and sealed with each other, and the third cleaning container  230  and the elution container  300  are bound. 
     2-4. Reaction Container 
     The reaction container  400  has substantially a cylindrical shape extending in the longitudinal direction of the container assembly  1  and constitutes a part of the flow path  2  of the container assembly  1 . The reaction container  400  has a reaction receiving section  404  formed on one opening end portion, a bottom  402  formed on the other closed end portion, and a reservoir section  406  that covers the reaction receiving section  404 . 
     The inner diameter of the reaction receiving section  404  is substantially equal to the outer diameter of the elution inserting section  302  of the elution container  300 . The elution inserting section  302  is inserted and fitted into the reaction receiving section  404 , and thereby the elution container  300  and the reaction container  400  are bound. 
     The reservoir section  406  having a predetermined space is provided on the periphery of the reaction receiving section  404 . The reservoir section  406  has a volume to receiving the liquid overflowing from the reaction container  400  due to movement of the plunger section  130 . 
     3. Content of Container Assembly and Operation of Container Assembly 
     Next, the content in the container assembly  1  will be described with reference to  FIG. 7A  and an operation of the container assembly  1  will be described with reference to  FIG. 7A  to  FIG. 8B .  FIGS. 7A and 7B  are views schematically illustrating the operation of the container assembly  1  according to the embodiment.  FIGS. 8A and 8B  are views schematically illustrating the operation of the container assembly  1  according to the embodiment. Further, in order to depict a state of the content, the respective containers are depicted as the flow path  2  and the external appearance and a binding structure are omitted in  FIG. 7A  to  FIG. 8B . 
     3-1. Content 
       FIG. 7A  illustrates a state of the content in the flow path  2  in the state in  FIG. 1 . The content in the flow path  2  is, in order from the cap  110  side to the reaction container  400 , an adsorption solution  10 , a first oil  20 , a first cleaning solution  12 , a second oil  22 , a second cleaning solution  14 , a third oil  24 , a magnetic bead  30 , a third oil  24 , a third cleaning solution  16 , a fourth oil  26 , an eluate  32 , a fourth oil  26 , and a reagent  34 . 
     In the flow path  2 , a portion (thick portion of the flow path  2 ) having a large cross-sectional area of a plane orthogonal to the longitudinal direction of the container assembly  1  and a portion (slim portion of the flow path  2 ) having a small cross-sectional area are alternately disposed. Apart or all of the first to fourth oils  20 ,  22 ,  24 , and  26  and the eluate  32  are accommodated in the thin portions of the flow path  2 . The cross-sectional area of the thin portion of the flow path  2  is an area in which, in a case where an interface between liquids (hereinafter, including a fluid) which are adjacent and not mixed to each other is disposed in the thin portion of the flow path  2 , the interface can be stably maintained. Accordingly, the liquid disposed in the thin portion of the flow path  2  enables a positional relationship between the liquid and the other liquids disposed on the upper and lower sides of the liquids to be stably maintained. In addition, even in a case where an interface between the liquid disposed in the thin portion of the flow path  2  and the other liquid disposed in the thick portion of the flow path  2  is formed in the thin portion of the flow path  2 , and the interface is stirred due to an impact, the flow path is placed in a stationary state and the interface is stably formed at a predetermined portion. 
     The thin portions of the flow path  2  are formed on the inner sides of the adsorption inserting section  122 , the first insertion section  212 , the second insertion section  222 , the third insertion section  232 , and the elution inserting section  302 , and extend upward over the elution inserting section  302  in the elution container  300 . Further, the liquids accommodated in the thin portions of the flow path  2  are stably maintained even before the container is assembled. 
     3-1-1. Oil 
     The first to fourth oils  20 ,  22 ,  24 , and  26  are all formed of oils and exist as the plugs between the liquids before and after the respective oils in the state in  FIGS. 7A and 7B . In order for the first to fourth oils  20 ,  22 ,  24 , and  26  to exist as the plugs, as the liquids adjacent before and after the respective oils, liquids which are phase-separated from each other, that is, liquids which are not mixed, are selected. In addition, the oil constituting the first to fourth oils  20 ,  22 ,  24 , and  26  may be different types of oils. The oil used for the first to fourth oils can be selected from, for example, silicone-based oil such as dimethyl silicone oil, paraffinic oil, mineral oil, and a compound thereof. 
     3-1-2. Adsorption Solution 
     The adsorption solution  10  indicates a liquid in which the nucleic acids are adsorbed to the magnetic bead  30 , for example, an aqueous solution containing a chaotropic agent. As the adsorption solution  10 , 5 M of guanidine thiocyanate, 2% of Triton X-100, or 50 mM of Tris-HCl (pH 7.2) can be used. As long as the adsorption solution  10  contains the chaotropic agent, there is no particular limitation to the adsorption solution; however, the adsorption solution  10  may contain a surfactant in order to break a cell membrane or to denature protein contained in a cell. As long as the surfactant is, in general, used for extracting the nucleic acids from a cell, or the like, there is no particular limitation to the surfactant; however, specifically, examples of the surfactant include a nonionic surfactant like a triton-based surfactant such as Triton-X or a tween-based surfactant such as Tween 20, an anionic surfactant such as N-Lauroylsarcosine sodium (SDS); however, particularly, it is preferable that nonionic surfactant is used in a range of 0.1% to 2%. Further, it is preferable that a reducing agent such as 2-mercaptoethanol or dithiothreitol is contained. A solution may be a buffer solution, and preferably a neutral solution with pH 6 to pH 8. In this respect, specifically, it is preferable that 3 M to 7 M of guanidine salt, 0% to 5% of a nonionic surfactant, 0 mM to 0.2 mM of EDTA, 0 M to 0.2 M of the reducing agent, or the like is contained. 
     Here, the chaotropic agent generates a chaotropic ion (monovalent anion having a large ion radius) in the aqueous solution and increases water solubility of a hydrophobic molecule. As long as the chaotropic agent contributes to adsorption of the nucleic acids to a solid-phase support, there is no particular limitation to the chaotropic agent. Specifically, examples of the chaotropic agent include guanidinium hydrochloride, sodium iodide, sodium perchlorate, or the like; however, it is preferable that guanidine thiocyanate or guanidinium hydrochloride, which actively denatures the protein, is used. The concentration of the chaotropic agent in a specification varies depending on the respective substances. For example, it is preferable that in a case where guanidine thiocyanate is used, the concentration is in a range of 3 M to 5.5 M and in a case where guanidinium hydrochloride is used, the concentration is equal to or more than 5 M. 
     The chaotropic agent exists in the aqueous solution, and thereby it is thermodynamically more advantageous that the nucleic acids in the aqueous solution are adsorbed on a solid than exist to be surrounded by water molecules, the nucleic acids are absorbed to the surface of the magnetic bead  30 . 
     3-1-3. Cleaning Solution 
     The first to third cleaning solutions  12 ,  14 , and  16  clean the magnetic bead  30  with which the nucleic acids are combined. 
     The first cleaning solution  12  is a liquid which is phase-separated from both the first oil  20  and the second oil  22 . The first cleaning solution  12  is preferably water or a low salt concentration aqueous solution, and preferably, a buffer solution in the case of the low salt concentration aqueous solution. Salt concentration of the low salt concentration aqueous solution is preferably equal to or lower than 100 mM, more preferably equal to or lower than 50 mM, and most preferably equal to or lower than 10 mM. In addition, the first cleaning solution  12  may contain the surfactant as described above, and there is no particular limitation to pH. In order to use the buffer solution as the first cleaning solution  12 , there is no particular limitation to the salt; however, it is preferable that Tris, HEPES, PIPES, phosphoric acid, or the like, is used. Further, it is preferable that the first cleaning solution  12  is contained by an amount with which adsorption of alcohol to a support of the nucleic acids, a reverse transfer reaction, the PCR reaction, or the like is not inhibited. In this case, there is no particular limitation to the concentration of the alcohol. 
     Further, the chaotropic agent may be contained in the first cleaning solution  12 . For example, when the guanidinium hydrochloride is contained in the first cleaning solution  12 , it is possible to clean the magnetic bead  30  or the like which maintains or strengthens adsorption of the nucleic acids which are adsorbed to the magnetic bead  30  or the like. 
     The second cleaning solution  14  is a liquid which is phase-separated from both the second oil  22  and the third oil  24 . The second cleaning solution  14  may have a composition which is the same as or different from that of the first cleaning solution  12 ; however, it is preferable that a solution, which virtually does not contain the chaotropic agent, is used. This is because the chaotropic agent does not infiltrate in the solution adjacent to the cleaning solution. As the second cleaning solution  14 , for example, 5 mM of Tris-HCl buffer may be used. As described above, it is preferable that the second cleaning solution  14  contains alcohol. 
     The third cleaning solution  16  is a liquid which is phase-separated from both the third oil  24  and the fourth oil  26 . Basically, the third cleaning solution  16  may have a composition which is the same as or different from that of the second cleaning solution  14 ; however, the cleaning solution does not contain alcohol. In addition, the third cleaning solution  16  can contain citric acid in order to prevent the alcohol from entering the reaction container  400 . 
     3-1-4. Magnetic Beads 
     The magnetic bead  30  is a bead which adsorbs the nucleic acids and it is preferable to have relatively strong magnetism such that a magnet  3  positioned outside the container assembly  1  causes the magnetic bead to move. The magnetic bead  30  may be, for example, a silica bead or a silica-coated bead. The magnetic bead  30  may be, preferably, the silica-coated bead. 
     3-1-5. Elution Solution 
     The eluate  32  is a liquid which is phase-separated from the fourth oil  26  and exists as a plug interposed between the fourth oils  26  and  26  in the flow path  2  in the elution container  300 . The eluate  32  is a liquid which elutes the nucleic acids adsorbed to the magnetic bead  30 , into the eluate  32  from the magnetic bead  30 . In addition, the eluate  32  forms a droplet in the fourth oil  26  through heating. As the eluate  32 , for example, pure water can be used. Here, the “droplet” means a liquid surrounded by a free surface. 
     3-1-6. Reagent 
     The reagent  34  contains a component required for reaction. In a case where the reaction in the reaction container  400  is the PCR, it is possible for the reagent  34  to contain at least one of enzymes and a primer (nucleic acid) such as a DNA polymerase for amplifying target nucleic acids (DNA) eluted in a droplet  36  (refer to  FIGS. 8A and 8B ) of the eluate, and a fluorescent probe that detects an amplified product. Here, all of the primer, the enzyme, and the fluorescent probe are contained. The reagent  34  is incompatible with the fourth oil  26  and is melted, is reacted when the reagent comes into contact with the droplet  36  of the eluate  32  which contains the nucleic acid, and exists in the lowermost region in a gravity direction of the flow path  2  in the reaction container  400  in a solid state. For example, the reagent  34  can use lyophilization (freeze-drying). 
     3-2. Operation of Container Assembly 
     An example of an operation of the container assembly  1  is described with respect to  FIG. 7A  to  FIG. 8B . 
     The operation of the container assembly  1  includes (A) a process of assembling the container assembly  1  by binding the adsorption container  100 , the cleaning container  200 , the elution container  300 , and the reaction container  400 , (B) a process of guiding a specimen containing the nucleic acids to the adsorption container  100  in which the adsorption solution  10  is accommodated, (C) a process of moving of the magnetic bead  30  from the second cleaning container  220  to the adsorption container  100 , (D) a process of oscillating the adsorption container  100  and adsorbing the nucleic acids to the magnetic bead  30 , (E) a process of moving of the magnetic bead  30 , to which the nucleic acids are adsorbed, to the elution container  300  from the adsorption container  100  through the first oil  20 , the first cleaning solution  12 , the second oil  22 , the second cleaning solution  14 , the third oil  24 , the third cleaning solution  16 , and the fourth oil  26 , in this order, (F) a process of eluting the nucleic acids from the magnetic bead  30  into the eluate  32  in the elution container  300 , and (G) a process of causing the droplet containing the nucleic acids to come into contact with the reagent  34  in the reaction container  400 . 
     Hereinafter, the respective process will be described in the order. 
     (A) Process of Assembling Container Assembly  1   
     As illustrated in  FIG. 7A , in the process of assembly, the adsorption container  100  to the reaction container  400  are bound and the container assembly  1  is assembled such that the flow path  2  which is continuous from the adsorption container  100  to the reaction container  400 . Further, in  FIG. 7A , the cap  110  is mounted in the adsorption container  100 ; however, the cap  110  is mounted on the plunger section  130  after (B) process. 
     More specifically, the elution inserting section  302  of the elution container  300  is inserted into the reaction receiving section  404  of the reaction container  400 , the third insertion section  232  of the third cleaning container  230  is inserted into the elution receiving section  304  of the elution container  300 , the second insertion section  222  of the second cleaning container  220  is inserted into the third reception section  234  of the third cleaning container  230 , the first insertion section  212  of the first cleaning container  210  is inserted into the second reception section  224  of the second cleaning container  220 , and the adsorption inserting section  122  of the adsorption container  100  is inserted into the first reception section  214  of the first cleaning container  210 . 
     (B) Process of Guiding Specimen 
     The process of guiding is performed by putting a cotton swab, to which, for example, a specimen is attached, in the adsorption solution  10  from the opening in which the cap  110  of the adsorption container  100  is mounted, and immersing the cotton swab in the adsorption solution  10 . More specifically, the cotton swab is put in from the opening as one end portion of the plunger section  130  which is in a state of being inserted into the syringe section  120  of the adsorption container  100 . Next, the cotton swab is taken out from the adsorption container  100  and the cap  110  is mounted. The state described above is shown in  FIG. 7A . In addition, the specimen may be guided to the adsorption container  100  by using a pipette, or the like. In addition, if the specimen is in a paste state or a solid state, for example, the specimen may be attached or may be input to the inner wall of the plunger section  130  to the adsorption container  100  by means of a spoon or tweezers. As illustrated in  FIG. 7A , the syringe section  120  and the plunger section  130  are filled with the adsorption solution  10  to an intermediate position; however, a space remains on the opening side on which the cap  110  is mounted. 
     The nucleic acids as the target are contained in the specimen. Hereinafter, the specimen is simply referred to as the target nucleic acids. For example, the target nucleic acids are DNA or RNA (DNA: deoxyribonucleic acid, and/or RNA: Ribonucleic acid). The target nucleic acids are used as a template of the PCR after the target nucleic acids are extracted from the specimen and are eluted to the eluate  32  to be described. Examples of the specimen include blood, nasal mucus, oral mucosa and other various biological samples. 
     (C) Process of Moving of Magnetic Bead 
     The process of moving of the magnetic bead  30  is performed by causing the magnet  3  to move toward the adsorption container  100  in a state in which a magnetic force of the magnet  3  disposed outside the container is applied to the magnetic bead  30  which exists to have the plug shape by being interposed between the third oils  24  and  24  of the second cleaning container  220  as illustrated in  FIG. 7A . 
     Along with the movement of the magnetic bead  30 , or by the moving of the cap  110  and the plunger section  130  in a direction of being drawn out from the syringe section  120  before the movement of the magnetic bead, the specimen in the adsorption solution  10  is caused to move to the syringe section  120  from the plunger section  130 . The movement of the plunger section  130  causes the flow path  2  closed by the distal end portion  134  to communicate with the adsorption solution  10 . 
     The magnetic bead  30  is lifted in the flow path  2  along with the movement of the magnet  3  and reaches the adsorption solution  10  in which the specimen is contained, as illustrated in  FIG. 7B . 
     (D) Process of Adsorbing Nucleic Acids to Magnetic Bead 
     The process of adsorbing the nucleic acids is performed by oscillating the adsorption container  100 . Since the opening of the adsorption container  100  is sealed by the cap  110  such that the adsorption solution  10  does not leak out, it is possible to efficiently perform the process. Through this process, the target nucleic acids are adsorbed to the surface of the magnetic bead  30  due to the action of the chaotropic agent. In the process, in addition to the target nucleic acid, nucleic acids or protein may be attached to the surface of the magnetic bead  30 . 
     As the method of oscillating the adsorption container  100 , a known device such as a vortex shaker may be used or an operator may manually perform the mixing. In addition, a magnetic field may be externally applied using the magnetism of the magnetic bead  30  and the adsorption container  100  may be oscillated. 
     (E) Process of Moving of Magnetic Bead to which Nucleic Acids are Adsorbed 
     In the process of moving of the magnetic bead  30  to which the nucleic acids are adsorbed, the magnetic force of the magnet  3  from the outside of the adsorption container  100 , the cleaning container  200 , and the elution container  300  is applied to cause the magnetic bead  30  to move in the adsorption solution  10 , the first to fourth oils  20 ,  22 ,  24 , and  26 , and the first to third cleaning solutions  12 ,  14 ,  16 . 
     AS the magnet  3 , for example, a permanent magnet, an electromagnet, or the like can be used. In addition, the magnet  3  may be caused to manually move by an operator, or the movement may be performed by using machinery equipment or the like. Since the magnetic bead  30  has properties of being attracted by the magnetic force, the magnetic bead changes relative disposition to the magnet  3  and moves inside the flow path  2  to the adsorption container  100 , the cleaning container  200 , and the elution container  300 . There is no particular limitation to a speed when the magnetic bead  30  passes through the respective cleaning solutions and the magnetic bead  30  may move by reciprocating in the same cleaning solution in the longitudinal direction of the flow path  2 . Further, in a case of causing particles other than the magnetic bead  30  to move in a tube, it is possible to perform the movement by using the gravity or a potential difference. 
     (F) Process of Eluting Nucleic Acids 
     In the process of eluting the nucleic acids, the nucleic acids are eluted from the magnetic bead  30  in the droplet  36  of the eluate in the elution container  300 . The eluate  32  in  FIGS. 7A and 7B  exists as the plug in the thin portions of the flow path of the elution container  300 ; however, the inside content expands due to the heating of the reaction container  400  during the movement of the magnetic bead  30  described above and the content moves upward in the elution container  300  as the droplet  36  as illustrated in  FIGS. 8A and 8B . Also, as illustrated in  FIG. 8A , when the magnetic bead  30  reaches the droplet  36  of the eluate of the elution container  300 , the target nucleic acids adsorbed to the magnetic bead  30  are eluted in the droplet  36  of the eluate due to an action of the eluate. 
     (G) Process of Coming into Contact with Reagent  34   
     In the process of coming into contact with the reagent  34 , the droplet  36  containing the nucleic acids is caused to come into contact with the reagent  34  positioned at the lowermost portion in the reaction container  400 . Specifically, as illustrated in  FIG. 8B , the cap  110  is pressed and the first oil  20  is pushed downward by the distal end portion  134  of the plunger section  130 . In this manner, the magnetic bead  30 , to which the magnetic force of the magnet  3  is applied, is maintained at the predetermined position, the droplet  36  of the eluate, in which the target nucleic acids are eluted, moves to the reaction container  400 , and the droplet comes into contact with the reagent  34  positioned at the lowermost portion of the reaction container  400 . The reagent  34 , with which the droplet  36  comes into contact, is melted and mixed with the target nucleic acids in the eluate, and, for example, it is possible to perform the PCR using the thermal cycle. 
     4. PCR Device 
     A PCR device  50  which performs nucleic acid eluting process and the PCR using the container assembly  1  is described with respect to  FIG. 9  and  FIG. 10 .  FIG. 9  is a diagram of a schematic configuration of a PCR device  50 .  FIG. 10  is a block diagram of the PCR device  50 . 
     The PCR device  50  includes a rotation mechanism  60 , a magnet moving mechanism  70 , a pressing mechanism  80 , a fluorescence measuring device  55 , and a controller  90 . 
     4-1. Rotation Mechanism 
     The rotation mechanism  60  includes a rotating motor  66  and a heater  65 , and driving of the rotating motor  66  causes the container assembly  1  and the heater  65  to rotate. The rotation mechanism  60  causes the container assembly  1  and the heater  65  to rotate and to be vertically reversed, and thereby the droplet containing the target nucleic acid in the flow path of the reaction container  400  moves and the thermal cycle processing is performed. 
     The heater  65  includes a plurality of heaters (not illustrated), and, for example, may include a heater for elution and a high temperature and a low temperature. The eluting heater heats the eluate having the plug shape of the container assembly  1  and promotes elution of the target nucleic acids from the magnetic bead to the eluate. The high-temperature heater heats the liquid on the upstream side in the flow path of the reaction container  400  to a temperature higher than a temperature heated by the low temperature heater. The low-temperature heater heats the bottom  402  of the flow path of the reaction container. It is possible to form a temperature gradient in the liquid in the flow path of the reaction container  400  by the high-temperature heater and the low-temperature heater. A temperature control device is provided in the heater  65  and it is possible to set the temperature of the liquid in the container assembly  1 , which is appropriate to a process, in response to an instruction from the controller  90 . 
     The heater  65  has an opening through which an outer wall of the bottom  402  of the reaction container  400  is exposed. The fluorescence measuring device  55  measures the luminance of the droplet of the eluate from the opening. 
     4-2. Magnet Moving Mechanism 
     The magnet moving mechanism  70  is a mechanism to cause the magnet  3  to move. The magnet moving mechanism  70  attracts the magnetic bead in the container assembly  1  to the magnet  3  and causes the magnetic bead to move in the container assembly  1  by causing the magnet  3  to move. The magnet moving mechanism  70  includes a pair of magnets  3 , a lifting and lowering mechanism, and an oscillating mechanism. 
     The oscillating mechanism is a mechanism that causes the pair of magnets  3  to oscillate in the right-left direction (or a front-rear direction in  FIG. 9 ) in  FIG. 9 . The pair of magnets  3  are disposed (refer to  FIG. 7A  to  FIG. 8B ) to interpose, in the right-left direction, the container assembly  1  mounted on the PCR device  50 , and it is possible to approach the magnetic bead and the magnet  3  in a direction (here, the right-left direction in  FIG. 9 ) orthogonal to the flow path of the container assembly  1 . Accordingly, when the pair of magnets  3  oscillate to follow an arrow in the right-left direction, the magnetic bead in the container assembly  1  moves in the right-left direction along with the movement. The lifting and lowering mechanism causes the magnet  3  to move in the vertical direction and it is possible to cause the magnetic bead to move in the vertical direction in  FIG. 9  along with the movement of the magnet  3 . 
     4-3. Pressing Mechanism 
     The pressing mechanism  80  is a mechanism of pressing the plunger section of the container assembly  1 . The plunger section is pressed by the pressing mechanism  80 , and thereby the droplet in the elution container  300  is extruded in the reaction container  400  and it is possible to perform the PCR in the reaction container  400 . 
     In  FIG. 9 , the pressing mechanism  80  is disposed above the upright container assembly  1 ; however, a direction in which the pressing mechanism  80  presses the plunger section may not be the vertical direction in  FIG. 9 , but may be inclined by 45 degrees with respect to the vertical direction. In this manner, it is easy to dispose the pressing mechanism  80  at a position at which the pressing mechanism does not interfere with the magnet moving mechanism  70 . 
     4-4. Fluorescence Measuring Device 
     The fluorescence measuring device  55  is a measuring device which measures the luminance of the droplet of the reaction container  400 . The fluorescence measuring device  55  is disposed at a position facing the bottom  402  of the reaction container  400 . Further, it is desirable that the fluorescence measuring device  55  can detect luminance in a plurality of wavelength bands so as to correspond to the multiplex PCRs. 
     4-5. Controller 
     The controller  90  is a control unit which performs control of the PCR device  50 . The controller  90  includes a processor such as a CPU and a storage device such as a ROM and a RAM. Various programs and data are stored in the storage device. In addition, the storage device provides a region in which the programs are extracted. A processor executes the programs stored in the storage device, and thereby various processes are realized. 
     For example, the controller  90  controls the rotating motor  66  such that the container assembly  1  rotates to a predetermined rotation position. A rotation position sensor (not illustrated) is provided in the rotation mechanism  60 , and the controller  90  drives and stops the rotating motor  66  in response to a detection result of the rotation position sensor. 
     In addition, the controller  90  controls the heater  65  such that ON/OFF control of the heater is performed, the heater generates heat, and the heater heats the liquid in the container assembly  1  to a predetermined temperature. 
     In addition, the controller  90  controls the magnet moving mechanism  70  such that the magnet  3  moves in the vertical direction and the magnet  3  oscillates in the right-left direction in  FIG. 9  in response to the detection result of the positional sensor (not illustrated). 
     In addition, the controller  90  controls the fluorescence measuring device  55  and measures the luminance of the droplet in the reaction container  400 . The measurement result is stored in the storage device (not illustrated) of the controller  90 . 
     The container assembly  1  is mounted on the PCR device  50 , it is possible to perform the processes of (C) to (G) in the above section 3-2, and further it is possible to perform the PCR. 
     5. Container and Liquid Storing Member 
     The container and the liquid storing member are described with reference to  FIG. 11  to  FIG. 16 . As long as the liquid can be sealed and stored therein, the container is applicable to various containers; however, here, a container constituting a part of the container assembly  1  described above is described. In addition, the liquid storing member includes the container in which the liquid is sealed and stored. 
     5-1. Elution Container 
     The container illustrated in  FIG. 11  to  FIG. 13  and  FIG. 16  is the elution container  300  which accommodates the eluate  32  as the liquid in a first flow path  2   a  and is the container described in “2-3. Elution Container” above. The elution container  300  has substantially a cylindrical shank  308  which forms a part of the first flow path  2   a  inside and extends in an axial direction of the first flow path  2   a , and two openings  310  and  330  at both ends of the first flow path  2   a.    
       FIG. 11  is a perspective view of the elution container  300  according to the embodiment.  FIG. 12  is a sectional view of a part of the elution container  300  on a first end portion  314 , which is taken along line B-B.  FIG. 13  is a sectional view of a part of the elution container  300  on a second end portion  334 , which is taken along line B-B.  FIG. 16  is a sectional view of a cartridge set  500  (the elution container  300  and the reaction container  400 ) according to the embodiment, which is taken along line B-B. Further, in the following description, the container and the liquid storing member are described without any particular distinction; however, the container is in a state in which no liquid is stored, and the liquid storing member has the container in which the liquid is sealed and stored. 
     As illustrated in  FIG. 11  to  FIG. 13 , the elution container  300  has the two openings  310  and  330 . First, with reference to  FIG. 12 , the first opening  310  of the elution container  300  on one end portion (first end portion  314  on the lower side in  FIG. 11 ) is described. 
     As illustrated in  FIG. 12 , the elution container  300  has the first opening  310  and is a container in which the fourth oil  26  and the eluate  32  ( FIG. 16 ) as the liquid are sealed and stored by sealing the first opening  310 . Further, since the eluate  32  is described in “3-1-5. Eluate”, the fourth oil is described in “3-1-1. Oil”, and thus repetitive description will be omitted. 
     The elution container  300  has the longitudinal direction, the first opening  310  is formed at one end portion (first end portion  314 ) of the elution container  300 , and the second opening  330  is formed at the other end portion (second end portion  334 ) of the elution container  300 . The elution container  300  has a first annular wall section  312  which is an annular wall section having an annular wall surface formed around the first opening  310 ; and a first attachment surface  318  which is formed on the inner side of the first annular wall section  312  and to which a first film  322  sealing the first opening  310  is attached. 
     The first opening  310  has a double-cylinder structure having a cylinder on the inner side and a cylinder on the outer side, in which the cylinder on the inner side is the elution inserting section  302  and the cylinder on the outer side is the first annular wall section  312 . The elution inserting section  302  and the first annular wall section  312  both have substantially a cylindrical shape. As long as the sections are tubular, any shapes may be employed. 
     The surface of the elution inserting section  302  on the inner side forms a part of the first flow path  2   a  and an opening end  303  of the elution inserting section  302  is formed at a position lower than the first attachment surface  318 . Here, “high” or “low” means that the opening is on the upper side and the portion, in which the liquid is accommodated, is the downward side, unless particularly noted otherwise in the present specification, and means being high and low of a height on the upper side of the respective portions of the container in a case where the container, to which a film has yet to be attached, is filled with a liquid. The opening end  303  is positioned lower than the first attachment surface  318 . Therefore, as illustrated in  FIG. 12 , when the filling with the fourth oil  26  is performed to the upper side of the first attachment surface  318 , the opening end  303  is submerged in the liquid. Accordingly, the opening end  303  of the elution inserting section  302  does not interfere with the first film when the first film  322  is attached. In addition, the elution inserting section  302  has an outer diameter which is the same as the shank  308 . An annular flange  320  protruding outward from the shank  308  is formed below the elution inserting section  302 . 
     The first annular wall section  312  extends upward from the top surface of the flange  320 . Here, “up” and “down” means the upward and downward direction in the drawings, unless particularly noted otherwise in the present specification. 
     The first annular wall section  312  has a height higher than the first attachment surface  318 . Here, “height” means a height on the upper side of the respective portions of the container in a case where the container, to which a film has yet to be attached, is filled with a liquid, unless particularly noted otherwise in the present specification. Accordingly, in a state in which the first opening  310  faces perpendicularly upward, the top edge (first end portion  314 ) of the first annular wall section  312  is positioned higher than the first attachment surface  318  in comparison between the first attachment surface  318  and the first annular wall section  312 . The first annular wall section  312  is positioned higher than the first attachment surface  318 , and thereby it is possible to perform filling with the fourth oil  26  to a position higher than the first attachment surface  318 . In this state, if an operation of attaching the first film  322  to the first attachment surface  318  is performed, it is possible to attach the first film  322  to the first attachment surface  318  in the liquid. Therefore, it is possible to prevent bubbles from being mixed to the liquid (fourth oil  26  and the eluate  32 ). 
     The first film  322  has sealing performance by which the elution container  300  can be sealed to store the liquid inside. In addition, the first film  322  has strength to the extent that the film is easily torn when binding to another container (reaction container  400 ) is performed. 
     The external appearance of the first film  322  is similar (in the present embodiment, circular shape) to the first inside surface  316  to be described below and is slightly smaller than the first inside surface. This is because it is easy to position the first film  322  to an attachment position by the first inside surface  316 . 
     It is possible to employ a known synthetic resin film as the first film  322 . In terms of thermal sealing to the first attachment surface  318 , it is preferable to use a film having a polyethylene layer on its surface or, for example, it is preferable to use a film having a multi-layer structure in which a polyethylene layer is laminated on the surface of the polyester film. 
     The first attachment surface  318  has an annular shape and is a surface to which the first film  322  is attached. The first attachment surface  318  is an annular step section which protrudes to the inner side and is formed on the first inside surface  316  which is the wall surface of the first annular wall section  312  on the inner surface. The first attachment surface  318  is an annular flat surface and is positioned to be lower than the first end portion  314 . In this manner, the first attachment surface  318  is the step section formed on the first inside surface  316 , and thereby it is possible to position the first film  322  on the first inside surface  316  and to attach the first film  322  to the first attachment surface  318  when the first film  322  is attached. In other words, since the movement of the first film  322  in the horizontal direction (in  FIG. 12 , in the right-left and front-rear directions) is limited by the first inside surface  316 , it is possible to reliably dispose the first attachment surface  318  at the predetermined attachment position. 
     Next, the second opening  330  of the elution container  300  on the other end portion (in  FIG. 11 , the second end portion  334  on the upper side) side of the elution container  300  will be described with reference to  FIG. 13 . 
     As illustrated in  FIG. 13 , the elution container  300  has the second opening  330  different from the first opening  310 , a second annular wall section  332  having an annular wall surface formed on the periphery of the second opening  330 , and a second annular attachment surface  338  to which a second film  340  sealing the second opening  330  is attached, on the inner side of the second annular wall section  332 . 
     The second annular wall section  332  has a height higher than the second attachment surface  338 . In this manner, the second annular wall section  332  and the second attachment surface  338  are also provided on the second end portion  334  side, it is possible to attach the film to the attachment surface in the liquid in both the openings  310  and  330 . Therefore, it is possible to prevent bubbles from being mixed to the liquid. The second annular wall section  332  is a part of the upper end of the elution receiving section  304 . 
     The elution receiving section  304  is a portion in which the third insertion section  232  of the third cleaning container  230  is received as described in “2-3. Elution Container”. 
     The second attachment surface  338  is an annular step section which protrudes to the inner side and is formed on a second inside surface  336  which is the annular inside surface of the elution receiving section  304 . The second attachment surface  338  has an annular shape and is a surface to which the second film  340  is attached. The second attachment surface  338  is an annular flat surface and is positioned to be lower than the second end portion  334 . In this manner, the second attachment surface  338  is the step section formed on the second inside surface  336 , and thereby it is possible to position the second film  340  on the second inside surface  336  and to attach the second film to the second attachment surface  338  when the second film  340  is attached. 
     As the second film  340 , it is possible to employ a film having the same function as the first film  322 . 
     5-2. Liquid Storing Member 
     As illustrated on the left side in  FIG. 16 , the elution container  300  in which the liquid (fourth oil  26  and eluate  32 ) is sealed and stored corresponds to the liquid storing member. 
     The first film  322  is attached to the first attachment surface  318  of the elution container  300 , the second film  340  is attached to the second attachment surface  338 , and the liquid (fourth oil  26  and eluate  32 ) is sealed and stored in the liquid storing member in the elution container  300  between the first film  322  and the second film  340 . As described in “5-1. Elution Container” above, according to the liquid storing member, it is possible to seal and store the liquid in a state in which bubbles are less likely to be mixed into the liquid. Further, elution container  300  has two openings, and thus has such a configuration described above; however, the reaction container  400  and the adsorption container  100  to be described below may have a structure in which the film is attached only on one opening on a side on which another container is bound. 
     In the liquid storing member, the elution inserting section  302  and the opening end  303  thereof are in the liquid of the fourth oil  26 . 
     5-3. Method of Manufacturing of Liquid Storing Member 
     A method of manufacturing the liquid storing member is described with reference to  FIG. 11  to  FIG. 13 . 
     First, as illustrated in  FIG. 11 , the elution container  300 , two openings  310  and  330  of which are not sealed, is prepared. 
     Next, as illustrated in  FIG. 13 , the second opening  330  faces perpendicularly upward and the second film  340  is mounted on the second attachment surface  338 . Since the movement of the second film  340  in the horizontal direction (in  FIG. 13 , in the right-left and front-rear directions) is limited by the second inside surface  336 , it is possible to easily dispose the second film at the predetermined position. Further, at this time, the first opening  310  is not sealed, and thus the fourth oil  26  does not exist unlike in  FIG. 13 . Filling with the fourth oil  26  as in  FIG. 13  is performed in a case where the first opening  310  is, at first, sealed. Further, the vicinity of the outer edge of the second film  340  is pressed on the second attachment surface  338  and is heated, and thereby the second film  340  is attached to the second attachment surface  338 . 
     Next, as illustrated in  FIG. 12 , the first opening  310  faces perpendicularly upward, the liquid (the fourth oil  26 , the eluate  32 , and the fourth oil  26 , entering in this order) is injected into the elution container  300 , and filling with the liquid (fourth oil  26 ) is performed to a level higher than the first attachment surface  318 . At this time, the first attachment surface  318  is positioned lower than the surface of the fourth oil  26  and is submerged in the liquid. 
     In this state, the first film  322  is mounted on the first attachment surface  318  in the liquid (position of a dotted line in the drawings). Since the movement of the first film  322  in the horizontal direction (in  FIG. 13 , in the right-left and front-rear directions) is limited by the first inside surface  316 , it is possible to easily dispose the first film at the predetermined position. Also, the vicinity of the outer edge of the first film  322  is pressed on the first attachment surface  318  and is heated to adhere to each other, and thereby the first film  322  is attached to the first attachment surface  318  and the liquid (fourth oil  26  and eluate  32 ) is stored in the sealed state. In this manner, the first film  322  is attached in the liquid, and thereby it is possible to prevent bubbles from being mixed in the liquid with which the filling is performed. A method of attachment of the first film  322  is preferably the adhesion; however, the attachment is not limited to the adhesion, but it is possible to employ another known attachment method. 
     In this manner, the elution container  300 , in which the fourth oil  26  and the eluate  32  are sealed and stored in the first flow path  2   a , is a liquid storing member in a state illustrated on the left side in  FIG. 16 . Further, an example, in which sealing is performed on the second opening  330  side in advance, is described; however, the sealing may be performed on the first opening  310  side in advance. In this case, similar to the first opening  310  described here, after the liquid is injected higher than the second attachment surface  338 , the second film  340  is attached. 
     5-4. Reaction Container 
     The container illustrated in  FIG. 14  to  FIG. 16  is the reaction container  400  in which the fourth oil  26  as the liquid is sealed and stored by sealing the third opening  410  and corresponds to the container described in “2-4. Reaction Container” above.  FIG. 14  is a perspective view of the reaction container  400  according to the embodiment.  FIG. 15  is a sectional view of a part of the reaction container  400  on the third end portion  414  side, which is taken along line B-B. 
     The reaction container  400  has a cylindrical shank  403  which forms a second flow path  2   b  inside, a third opening  410  formed on one end portion, the bottom  402  which closes the second flow path  2   b  formed at the other end portion, the cylindrical reservoir section  406  formed on the periphery of the shank  403  on the third opening  410  side, and a reaction receiving section  404  in which the elution inserting section  302  is received in the second flow path  2   b . The second flow path  2   b  contains the fourth oil  26  and the reagent  34  which reacts with the nucleic acids eluted in the eluate  32 . 
     The reaction container  400  has a third annular wall section  412  having an annular wall surface on the third end portion  414  side, as one end portion, and a third annular attachment surface  418  which is formed on the inner side of the third annular wall section  412  and to which a third film  422  sealing the third opening  410  is attached. 
     Similar to the first opening  310 , the third opening  410  has the double-cylinder structure in which the cylinder on the inner side is the reaction receiving section  404  and the cylinder on the outer side is the third annular wall section  412 . When the elution container  300  is bound to the reaction container  400 , a space between the outside surfaces of the third annular wall section  412  and the reaction receiving section  404  can receive a liquid which leaks out from the flow paths  2   a  and  2   b  such that the liquid does not leak to the outside with the first film  322  and the third film  422  being torn. 
     The inner surface of the reaction receiving section  404  forms a part of the second flow path  2   b  and has an outer diameter which is substantially the same as the shank  403 . An annular connection section  420  protruding outward from the shank  308  is formed below the reaction receiving section  404 . The third annular wall section  412  extends upward from the outer circumferential edge of the connection section  420 . The reaction receiving section  404  and the third annular wall section  412  have both substantially a cylindrical shape. However, as long as the sections are tubular, any shapes may be employed. 
     The third attachment surface  418  has an annular shape and is a surface to which the third film  422  is attached. The third attachment surface  418  is the top surface of the reaction receiving section  404 . The third attachment surface  418  is an annular flat surface. 
     The third annular wall section  412  has a height higher than the third attachment surface  418 . Accordingly, in a state in which the third opening  410  faces perpendicularly upward, the top edge (third end portion  414 ) of the third annular wall section  412  is positioned higher than the third attachment surface  418 , in comparison between the third attachment surface  418  and the third annular wall section  412 . The third annular wall section  412  is positioned higher than the third attachment surface  418 , and thereby it is possible to perform filling with the fourth oil  26  to a position higher than the third attachment surface  418 . In this state, if an operation of attaching the third film  422  to the third attachment surface  418  is performed, it is possible to attach the third film  422  to the third attachment surface  418  in the liquid. Therefore, it is possible to prevent bubbles from being mixed to the fourth oil  26 . 
     As the third film  422 , it is possible to employ a film having the same function as the first film  322 . The third film  422  has the external appearance of a circular shape and has an outer diameter greater than that of the third attachment surface  418 . In a state in which the third film  422  is attached to the third attachment surface  418 , the outer circumferential edge of the third film  422  has a gap with the third inside surface  416 . 
     5-5. Another Liquid Storing Member 
     As illustrated in  FIG. 16 , the reaction container  400  in which another liquid (fourth oil  26 ) is sealed and stored corresponds to another liquid storing member. In the other liquid storing member, the third film  422  is attached to the third attachment surface  418  of the reaction container  400 , and the liquid (fourth oil  26 ) is sealed and stored in the reaction container  400 . As described in “5-4. reaction container” above, according to the other liquid storing member, it is possible to perform sealing to store the liquid in a state in which bubbles are less likely to be mixed in the liquid. 
     5-6. Method of Manufacturing of Liquid Storing Member 
     A method of manufacturing the other liquid storing member is described with reference to  FIG. 14  and  FIG. 15 . 
     First, as illustrated in  FIG. 14 , the reaction container  400 , the third opening  410  of which is not sealed, is prepared. 
     Next, as illustrated in  FIG. 15 , the third opening  410  faces perpendicularly upward, the liquid (the reagent  34  and the fourth oil  26 ) is injected into the reaction container  400 , and filling with the liquid (fourth oil  26 ) is performed to a level higher than the third attachment surface  418 . At this time, the third attachment surface  418  is positioned lower than the surface of the fourth oil  26  and is submerged in the liquid. 
     The third film  422  is mounted on the third attachment surface  418  in the liquid (position of a dotted line in the drawings). Also, the vicinity of the outer edge of the third film  422  is pressed on the third attachment surface  418  and is heated, and thereby the third film  422  is attached to the third attachment surface  418  such that the liquid (fourth oil  26  and reagent  34 ) is stored in the sealed state. In this manner, the third film  422  is attached in the liquid, and thereby it is possible to prevent bubbles from being mixed in the stored liquid in the sealed state. 
     In this manner, the reaction container  400 , in which the fourth oil  26  and the reagent  34  are sealed and stored in the second flow path  2   b , is a liquid storing member in a state illustrated on the right side in  FIG. 16 . 
     6. Cartridge Set 
     The cartridge set  500  is described with reference to  FIG. 16 .  FIG. 16  is a sectional view of the cartridge set  500  according to an embodiment, which is taken along line B-B. 
     As illustrated in  FIG. 16 , the cartridge set  500  includes one liquid storing member as the elution container  300  described in “5-2. Liquid Storing Member” above and the other liquid storing member as the reaction container  400  described in “5-5. Another Liquid Storing Member” above, which is bound to the liquid storing member. 
     The liquid storing member as the elution container  300  can be bound to the other liquid storing member as the reaction container  400  such that the elution inserting section  302  is inserted into the reaction receiving section  404  in an insertion direction S. Also, at the time of a binding operation, the first film  322  and the third film  422  are torn by the opening end  303  of the elution inserting section  302  and the third attachment surface  418  of the reaction receiving section  404 . Since the opening end  303  of the elution inserting section  302  is inserted in the reaction receiving section  404  which is fully filled with the fourth oil  26 , in the liquid as is, it is difficult for bubbles to be mixed into between (a flow path which is formed to communicate with the two flow paths) the first flow path  2   a  and the second flow path  2   b  even during the binding operation. The binding state is illustrated in  FIG. 17 .  FIG. 17  is a sectional view of a part of a nuclei acid amplifying reaction cartridge  502 , which is taken along line B-B. Further, in  FIG. 17 , the film is not shown. 
     In addition, as described above, according to the cartridge set  500 , it is possible to bind two liquid storing member, in which the liquids are sealed and stored in the state in which bubbles are less likely to be mixed therein, and it is possible to assemble the nuclei acid amplifying reaction cartridge  502 . 
     The cartridge set  500  ( FIG. 16 ) described above is a set of the elution container  300  and the reaction container  400 , but can be assembled with an appropriate combination of the other containers ( 100 ,  200 ,  300 , and  400 ) described above. The sealing structure of both the openings of the elution container  300  can be similarly employed to the first cleaning container  210 , the second cleaning container  220 , and the third cleaning container  230 . In the case of the first and second cleaning containers  210  and  220 , a plate-shaped member protrudes from the first and second insertion sections  212  and  222  ( FIG. 6 ); however, similarly, a film is attached to the plate-shaped member as in the liquid. In addition, it is possible to employ the sealing structure in the first opening  310  of the elution container  300  to the end portion which is bound to the first cleaning container  210  in the adsorption container  100  and it is possible to insert the plunger section  130 , to which the film is attached, into the other opening. Also, these containers ( 100 ,  200 ,  300 , and  400 ) are bound, and thereby it is possible to obtain the container assembly  1  described above. Further, it is possible to appropriately alter the binding process order of the respective containers and it is possible to appropriately alter the combination between the containers. 
     The invention is not limited to the embodiments described above and further can be variously modified. For example, the invention includes substantially the same configuration (for example, configuration having the same function, method, and result, or configuration having the same object and effects) as the configuration described in the embodiments. In addition, the invention includes a configuration in which a portion, which is not a fundamental portion of the configuration described in the embodiments, is replaced. In addition, the invention includes a configuration in which the same effect is achieved, or a configuration in which the same object is achieved, as that of the configuration described in the embodiments. In addition, the invention includes a configuration in which a known technology is added to the configuration described in the embodiments. 
     The entire disclosure of Japanese Patent Application No. 2015-031625, filed Feb. 20, 2015 is expressly incorporated by reference herein.