Patent Publication Number: US-5631273-A

Title: Synergistic water soluble preservative compositions of biocidal mixtures

Description:
CROSS-REFERENCE TO RELATED PATENT APPLICATION 
     This is a division of application Ser. No. 08/413,742, filed Mar. 30, 1995, now U.S. Pat. No. 5,552,425, which is a continuation-in-part of Ser. No. 08/234,089, filed Apr. 28, 1994 now U.S. Pat. No. 5,428,050. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     This invention relates to preservative compositions, and, more particularly, to a water soluble antibacterial compositions for addition to commercial use products to provide long time synergistic biocidal activity therein, which are active liquid blends of an admixture of a methylol compound and an iodopropynyl compound, in a predetermined weight ratio of 100:1 to 2000:1, and propylene glycol or 1,3-butylene glycol. 
     2. Description of the Prior Art 
     Combinations of antimicrobial agents have been developed in the prior art in order to: 
     (1) produce a biochemical synergism; 
     (2) broaden the antimicrobial spectrum of activity of each agent; 
     (3) increase water solubility for the admixture; 
     (4) minimize the toxicity or irritation of a given agent to the host; and 
     (5) minimize physical and chemical incompatibilities. 
     True biological synergism exists when two agents, when combined, require lesser amounts of the agents to bring about the same inhibitory or cidal effect than either single agent alone. While synergistic interaction for two or more antimicrobial agents does produce more than merely an additive effect in the resultant biological activity, in most cases the mechanism of such synergism remains a mystery. 
     M. Rosen et al., in U.S. Pat. No. 4,844,891, for example, described a preservative admixture of (a) a formaldehyde donor and (b) a halopropynyl compound, in a weight ratio of (a):(b) of 50:1 to 1:1, preferably 30:1 to 2:1, and, most preferably, 20:1 to 10:1, as providing fungicidal activity for 1-3 days in commercial use formulations. However, Rosen observed that when the ratio of (a):(b) in the concentrate exceeded 50:1 (System No. 16 in Table 1, a ratio of 73.33), the preservative composition was ineffective in providing biocidal protection in the use formulations. Thus a relatively large amount of the halopropynyl compound was required by Rosen to provide significant biocidal activity in the use composition. In such admixtures, although the formaldehyde donor is water soluble, the halopropynyl compound is substantially insoluble in water. Therefore it was difficult for Rosen to uniformly distribute his admixture throughout the use composition. 
     For these and other reasons, it is desired to provide a new and improved water soluble preservative admixture of such biocidal compounds which requires relatively little of the water insoluble and expensive halopropynyl compound, and that also provides effective synergistic protection in use formulations against a wide range of fungi and bacteria at different use levels over a long period of time. 
     A feature of the present invention is the provision of an admixture concentrate which is water soluble and which therefore can be uniformly distributed in use compositions at a predetermined use level. 
     Another feature herein is the provision of a preservative admixture which exhibits a long term synergistic biocidal activity against wide range of fungi and both gram-negative and gram-positive bacteria in the use compositions. 
     Still another feature of this invention is the provision of a water soluble preservative antibacterial composition for addition to commercial use products at predetermined use levels against a wide range of fungi and gram-negative and gram-positive bacteria, in the form of an active liquid blend of an admixture of one or more methylol compounds and an iodopropynyl compound, in a predetermined weight ratio of 100:1 to 2000:1, and propylene glycol or 1,3-butylene glycol. 
     These and other objects and features of the invention will be made apparent from the following more particular description of the invention. 
     SUMMARY OF THE INVENTION 
     What has been discovered is a water soluble preservative composition for addition to commercial use compositions at predetermined use levels, and uniformly distributed therein, which provides long term synergistic biocidal activity against a wide range of fungi and both gram-negative and gram-positive bacteria. The composition of the invention comprises: 
     (1) an admixture of powders of 
     (a) a methylol compound, or their equivalent, and 
     (b) iodopropynyl alcohol, or its ester, carbamate or ether derivative thereof, 
     in a weight ratio of (a):(b) of 100:1 to 2000:1, preferably 200:1 to 500:1; and 
     (2) propylene glycol or 1,3-butylene glycol. 
     Suitably, the composition of the invention comprises about 20 to 55 weight percent of (1) and 45-80 weight percent of (2), preferably 40 weight percent of (1) and 60 weight percent of (2). 
     Commercial use compositions containing about 0.1 to 5% by weight, preferably 0.5-1%, of the composition of the invention also are provided therein. Such use compositions contain an iodopropynyl compound in an amount necessary to provide the desired antifungal activity, and a methylol compound in an amount necessary to provide the desired antibacterial activity for the user. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The invention is based upon the discoveries that in a predetermined admixture of (a) a methylol compound, or its equivalent, and (b) an iodopropynyl compound: 
     (1) Iodopropynyl compounds are substantially water insoluble at weight ratios of (a):(b) of less than 100; accordingly, at weight ratios below 100:1, it is difficult to uniformly distribute the iodopropynyl compound in aqueous use compositions, particularly in creams, gels and the like. In this invention, the admixtures are used at a weight ratio of (a):(b) of 100:1 to 2000:1, which are water soluble in all use compositions at conventional use levels. 
     (2) Effective synergistic biocidal activity is achieved for admixtures having a weight ratio of (a):(b) of 100:1 to 2000:1. Such admixtures have a Synergistic Index (SI) value approaching zero (maximum synergism) for a wide range of organisms. In contrast, admixtures with (a):(b) ratios below 100:1, e.g. 10:1 to 50:1, are much less synergistic, and are active with only a narrower range of organisms. 
     (3) A water soluble preservative antibacterial composition having enhanced activity can be made available in the form of a liquid blend of an admixture of one or more methyol compounds and 3-iodo-2-propynylbutyl carbamate, in a weight ratio of 100:1 to 2000:1, and propylene glycol or 1,3-butylene glycol, in a predetermined formulation of 20-55 weight percent of the admixture and 45-80 weight percent of the glycol, preferably 40:60. 
     (4) Preservative activity for use compositions is achieved most effectively with an admixture wt. ratio of 100:1 to 2000:1 at use levels of the composition of 0.1 to 5%, preferably 0.5 to 1%, by weight of the finished product. In this amount, the antifungal iodopropynyl compound is present in a small amount which provides antifungal protection and which significantly reduces the cost and toxicity of the use composition, and the antibacterial methylol compound is present in an amount which is sufficient to provide the desired antibacterial protection. 
     The experimental results upon which these discoveries are based are described below. In these examples, the (a) methylol compound may be selected from diazolidinyl urea (GERMALL® II) N-[1,3-bis(hydroxymethyl)-2,5-dioxo-4-imidazolidinyl]-N,N&#39;-bis(hydroxymethyl) urea, imidurea (GERMALL® 115), 1,1-methylene bis[3-[3-(hydroxymethyl)-2,5-dioxo-4-imidazolidinyl]urea]; 1,3-dimethylol-5,5-dimethyl hydantoin (DMDMH), sodium hydroxymethyl glycinate (SUTTOCIDE® A), and glycine anhydride dimethylol (GADM); the (b) iodopropynyl compound is 3-iodo-2-propynylbutyl carbamate (IPBC); and the (c) glycol is propylene glycol or 1,3-butylene glycol. 
     1. WATER SOLUBILITY 
     The water solubility or insolubility of admixtures of several methylol compounds with IPBC as 1% aqueous solutions is shown in Tables A through C below. 
     
                       TABLE A                                                     
______________________________________                                    
Weight Ratio of                                                           
Germall ® II:IPBC                                                     
                Amount in ppm                                             
                            Solubility                                    
______________________________________                                    
2000:1           5          Soluble                                       
1000:1          10          Soluble                                       
500:1           20          Soluble                                       
200:1           50          Soluble                                       
100:1           100         Soluble                                       
 50:1           200         Insoluble                                     
 20:1           500         Insoluble                                     
______________________________________                                    
 
    
     
                       TABLE B                                                     
______________________________________                                    
Weight Ratio of                                                           
GADM:IPBC      Amount in ppm                                              
                           Solubility                                     
______________________________________                                    
2000:1          5          Soluble                                        
1000:1         10          Soluble                                        
500:1          20          Soluble                                        
200:1          50          Soluble                                        
100:1          100         Soluble                                        
 50:1          200         Insoluble                                      
 20:1          500         Insoluble                                      
______________________________________                                    
 
    
     
                       TABLE C                                                     
______________________________________                                    
Weight Ratio of                                                           
DMDMH:IPBC     Amount in ppm                                              
                           Solubility                                     
______________________________________                                    
2000:1          5          Soluble                                        
1000:1         10          Soluble                                        
500:1          20          Soluble                                        
200:1          50          Soluble                                        
100:1          100         Soluble                                        
 50:1          200         Insoluble                                      
 20:1          500         Insoluble                                      
______________________________________                                    
 
    
     These results demonstrate that admixtures having a ratio of 50:1 or 20:1 are insoluble in water whereas at ratios of 100:1 to 2000:1 the admixtures are soluble in water. 
     2. SYNERGISM 
     Tables 1 through 14 below demonstrate the very effective synergistic interaction between compounds &#34;a&#34; and &#34;b&#34; against a broad range of fungi and both gram-negative and gram-positive bacteria. The following organisms were tested: 
     
         ______________________________________                                    
             ATCC                                                         
Organism     Number   Inoculum Concentration                              
______________________________________                                    
Ps. aeruginosa (PSA)*                                                     
              9027    2.1 × 10.sup.6 CFU/gm of Product              
E. coli (ECOLI)*                                                          
              8739    4.7 × 10.sup.5 CFU/gm of Product              
Staph. aureus (SA)**                                                      
              6538    1.6 × 10.sup.6 CFU/gm of Product              
Ps. cepacia (PC)*                                                         
             25416    1.6 × 10.sup.6 CFU/gm of Product              
C. albicans (CAN)***                                                      
             10231    8.0 × 10.sup.4 CFU/gm of Product              
A. niger (AN)***                                                          
             16404    2.7 × 10.sup.5 CFU/gm of Product              
______________________________________                                    
 *gram-negative bacteria                                                  
 **grampositive bacteria                                                  
 ***fungi                                                                 
 
    
     Table D below lists the static (MIC) and cidal activities of several antimicrobial agents in ppm. These activities are used to calculate the Synergism Index (SI) of admixtures of the present invention. 
     
                                           TABLE D                                 
__________________________________________________________________________
Static (MIC) and Cidal Activities of Several Antimicrobial Compounds      
(Static/Cidal Concentrations in ppm)                                      
Organism                                                                  
(ATCC #) IPBC  Germall ® II                                           
                      GADM   DMDMH  IPGA                                  
__________________________________________________________________________
(SA) (6538)                                                               
         100/200                                                          
                400/1600                                                  
                      400/800                                             
                              450/1600                                    
                                     300/5000                             
(ECOLI) (8739)                                                            
          50/100                                                          
                400/1600                                                  
                      400/800                                             
                             400/800                                      
                                    150/600                               
(PSA) (9027)                                                              
          800/1200                                                        
                400/1600                                                  
                      400/400                                             
                              600/1600                                    
                                    70/70                                 
(PC) (25416)                                                              
         1200/1800                                                        
               200/400                                                    
                      200/400                                             
                              600/1600                                    
                                     70/300                               
(CAN) (10231)                                                             
          50/100                                                          
                1500/15000                                                
                       7500/15000                                         
                              8000/16000                                  
                                     50/300                               
(AN) (16404)                                                              
          50/100                                                          
               3200/3200                                                  
                      1600/3200                                           
                              8000/16000                                  
                                    30/30                                 
__________________________________________________________________________
 
    
     The Synergism Index was determined by the same mathematical treatment of such data described by Kull et al. in Applied Microbiology 9, 538-541 (1961) using the following relationship: ##EQU1## where: 1. Q a  =The quantity of Compound a acting alone, producing an endpoint. 
     2. Q b  =The quantity of Compound b acting alone, producing and endpoint. 
     3. Q A  =The quantity of Compound A in mixture, producing an endpoint. 
     4. Q B  =The quantity of Compound B in mixture, producing an endpoint. 
     When SI is equal to 1, a mere additive effect of the components in the mixture is indicated; when SI is less than 1, synergism has occurred; and when SI is greater than 1 it indicates antagonism of the two components. 
     According to this well known method of measuring synergism, the quantity of each component in the various mixtures is compared with the quantity of pure component that is required to reach the same endpoint or to produce the same microbiological effect as the mixture. 
     
                       TABLE 1                                                     
______________________________________                                    
2000:1 Wt. Ratio GERMALL ® II/IPBC                                    
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  0.05 99.95  0.06                          
&#34;        ECOLI    100     1600  0.05 99.95  0.06                          
&#34;        PSA      1200    1600  0.05 99.95  0.06                          
&#34;        PC       1800    1250  0.05 99.95  0.08                          
&#34;        CAN      100     15000 0.05 99.95  0.01                          
&#34;        AN       100     3200  0.05 99.95  0.03                          
 0.025%  SA       200     1600  0.125                                     
                                     249.9  0.16                          
&#34;        ECOLI    100     1600  0.125                                     
                                     249.9  0.16                          
&#34;        PSA      1200    1600  0.125                                     
                                     249.9  0.16                          
&#34;        PC       1800    1250  0.125                                     
                                     249.9  0.20                          
&#34;        CAN      100     15000 0.125                                     
                                     249.9  0.02                          
&#34;        AN       100     3200  0.125                                     
                                     249.9  0.08                          
0.05%    SA       200     1600  0.25 499.8  0.31                          
&#34;        ECOLI    100     1600  0.25 499.8  0.31                          
&#34;        PSA      1200    1600  0.25 499.8  0.31                          
&#34;        PC       1800    1250  0.25 499.8  0.40                          
&#34;        CAN      100     15000 0.25 499.8  0.04                          
&#34;        AN       100     3200  0.25 499.8  0.16                          
0.10%    SA       200     1600  0.5  999.5  0.63                          
&#34;        ECOLI    100     1600  0.5  999.5  0.63                          
&#34;        PSA      1200    1600  0.5  999.5  0.63                          
&#34;        PC       1800    1250  0.5  999.5  0.80                          
&#34;        CAN      100     15000 0.5  999.5  0.07                          
&#34;        AN       100     3200  0.5  999.5  0.32                          
0.20%    SA       200     1600  1    1999   1.25                          
&#34;        ECOLI    100     1600  1    1999   1.26                          
&#34;        PSA      1200    1600  1    1999   1.25                          
&#34;        PC       1800    1250  1    1999   1.60                          
&#34;        CAN      100     15000 1    1999   0.14                          
&#34;        AN       100     3200  1    1999   0.63                          
0.40%    SA       200     1600  2    3998   2.51                          
&#34;        ECOLI    100     1600  2    3998   2.52                          
&#34;        PSA      1200    1600  2    3998   2.50                          
&#34;        PC       1800    1250  2    3998   3.20                          
&#34;        CAN      100     15000 2    3998   0.29                          
&#34;        AN       100     3200  2    3998   1.27                          
0.50%    SA       200     1600  2.5  4997.5 3.14                          
&#34;        ECOLI    100     1600  2.5  4997.5 3.15                          
&#34;        PSA      1200    1600  2.5  4997.5 3.13                          
&#34;        PC       1800    1250  2.5  4997.5 4.00                          
&#34;        CAN      100     15000 2.5  4997.5 0.36                          
&#34;        AN       100     3200  2.5  4997.5 1.59                          
______________________________________                                    
 
    
     
                       TABLE 2                                                     
______________________________________                                    
1000:1 Wt. Ratio GERMALL ® II/IPBC                                    
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  0.1  99.9   0.06                          
&#34;        ECOLI    100     1600  0.1  99.9   0.06                          
&#34;        PSA      1200    1600  0.1  99.9   0.06                          
&#34;        PC       1800    1250  0.1  99.9   0.08                          
&#34;        CAN      100     15000 0.1  99.9   0.01                          
&#34;        AN       100     3200  0.1  99.9   0.03                          
 0.025%  SA       200     1600  0.25 249.8  0.16                          
&#34;        ECOLI    100     1600  0.25 249.8  0.16                          
&#34;        PSA      1200    1600  0.25 249.8  0.16                          
&#34;        PC       1800    1250  0.25 249.8  0.20                          
&#34;        CAN      100     15000 0.25 249.8  0.02                          
&#34;        AN       100     3200  0.25 249.8  0.08                          
0.05%    SA       200     1600  0.5  499.5  0.31                          
&#34;        ECOLI    100     1600  0.5  499.5  0.32                          
&#34;        PSA      1200    1600  0.5  499.5  0.31                          
&#34;        PC       1800    1250  0.5  499.5  0.40                          
&#34;        CAN      100     15000 0.5  499.5  0.04                          
&#34;        AN       100     3200  0.5  499.5  0.16                          
0.10%    SA       200     1600  1    999    0.63                          
&#34;        ECOLI    100     1600  1    999    0.63                          
&#34;        PSA      1200    1600  1    999    0.63                          
&#34;        PC       1800    1250  1    999    0.80                          
&#34;        CAN      100     15000 1    999    0.08                          
&#34;        AN       100     3200  1    999    0.32                          
0.20%    SA       200     1600  2    1998   1.26                          
&#34;        ECOLI    100     1600  2    1998   1.27                          
&#34;        PSA      1200    1600  2    1998   1.25                          
&#34;        PC       1800    1250  2    1998   1.60                          
&#34;        CAN      100     15000 2    1998   0.15                          
&#34;        AN       100     3200  2    1998   0.64                          
0.40%    SA       200     1600  4    3996   2.52                          
&#34;        ECOLI    100     1600  4    3996   2.54                          
&#34;        PSA      1200    1600  4    3996   2.50                          
&#34;        PC       1800    1250  4    3996   3.20                          
&#34;        CAN      100     15000 4    3996   0.31                          
&#34;        AN       100     3200  4    3996   1.29                          
0.50%    SA       200     1600  5    4995   3.15                          
&#34;        ECOLI    100     1600  5    4995   3.17                          
&#34;        PSA      1200    1600  5    4995   3.13                          
&#34;        PC       1800    1250  5    4995   4.00                          
&#34;        CAN      100     15000 5    4995   0.38                          
&#34;        AN       100     3200  5    4995   1.61                          
______________________________________                                    
 
    
     
                       TABLE 3                                                     
______________________________________                                    
500:1 Wt. Ratio GERMALL ® II/IPBC                                     
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  0.2  99.8   0.06                          
&#34;        ECOLI    100     1600  0.2  99.8   0.06                          
&#34;        PSA      1200    1600  0.2  99.8   0.06                          
&#34;        PC       1800    1250  0.2  99.8   0.08                          
&#34;        CAN      100     15000 0.2  99.8   0.01                          
&#34;        AN       100     3200  0.2  99.8   0.03                          
 0.025%  SA       200     1600  0.5  249.5  0.16                          
&#34;        ECOLI    100     1600  0.5  249.5  0.16                          
&#34;        PSA      1200    1600  0.5  249.5  0.16                          
&#34;        PC       1800    1250  0.5  249.5  0.20                          
&#34;        CAN      100     15000 0.5  249.5  0.02                          
&#34;        AN       100     3200  0.5  249.5  0.08                          
0.05%    SA       200     1600  1    499    0.32                          
&#34;        ECOLI    100     1600  1    499    0.32                          
&#34;        PSA      1200    1600  1    499    0.31                          
&#34;        PC       1800    1250  1    499    0.40                          
&#34;        CAN      100     15000 1    499    0.04                          
&#34;        AN       100     3200  1    499    0.17                          
0.10%    SA       200     1600  2    998    0.63                          
&#34;        ECOLI    100     1600  2    998    0.64                          
&#34;        PSA      1200    1600  2    998    0.63                          
&#34;        PC       1800    1250  2    998    0.80                          
&#34;        CAN      100     15000 2    998    0.09                          
&#34;        AN       100     3200  2    998    0.33                          
0.20%    SA       200     1600  4    1996   1.27                          
&#34;        ECOLI    100     1600  4    1996   1.29                          
&#34;        PSA      1200    1600  4    1996   1.25                          
&#34;        PC       1800    1250  4    1996   1.60                          
&#34;        CAN      100     15000 4    1996   0.17                          
&#34;        AN       100     3200  4    1996   0.66                          
0.40%    SA       200     1600  8    3992   2.54                          
&#34;        ECOLI    100     1600  8    3992   2.58                          
&#34;        PSA      1200    1600  8    3992   2.50                          
&#34;        PC       1800    1250  8    3992   3.20                          
&#34;        CAN      100     15000 8    3992   0.35                          
&#34;        AN       100     3200  8    3992   1.33                          
0.50%    SA       200     1600  10   4990   3.17                          
&#34;        ECOLI    100     1600  10   4990   3.22                          
&#34;        PSA      1200    1600  10   4990   3.13                          
&#34;        PC       1800    1250  10   4990   4.00                          
&#34;        CAN      100     15000 10   4990   0.43                          
&#34;        AN       100     3200  10   4990   1.66                          
______________________________________                                    
 
    
     
                       TABLE 4                                                     
______________________________________                                    
200:1 Wt. Ratio GERMALL ® II/IPBC                                     
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  0.5  99.5   0.06                          
&#34;        ECOLI    100     1600  0.5  99.5   0.07                          
&#34;        PSA      1200    1600  0.5  99.5   0.06                          
&#34;        PC       1800    1250  0.5  99.5   0.08                          
&#34;        CAN      100     15000 0.5  99.5   0.01                          
&#34;        AN       100     3200  0.5  99.5   0.04                          
 0.025%  SA       200     1600  1.25 248.75 0.16                          
&#34;        ECOLI    100     1600  1.25 248.75 0.17                          
&#34;        PSA      1200    1600  1.25 248.75 0.16                          
&#34;        PC       1800    1250  1.25 248.75 0.20                          
&#34;        CAN      100     15000 1.25 248.75 0.03                          
&#34;        AN       100     3200  1.25 248.75 0.09                          
0.05%    SA       200     1600  2.5  497.5  0.32                          
&#34;        ECOLI    100     1600  2.5  497.5  0.34                          
&#34;        PSA      1200    1600  2.5  497.5  0.31                          
&#34;        PC       1800    1250  2.5  497.5  0.40                          
&#34;        CAN      100     15000 2.5  497.5  0.06                          
&#34;        AN       100     3200  2.5  497.5  0.18                          
0.10%    SA       200     1600  5    995    0.65                          
&#34;        ECOLI    100     1600  5    995    0.67                          
&#34;        PSA      1200    1600  5    995    0.63                          
&#34;        PC       1800    1250  5    995    0.80                          
&#34;        CAN      100     15000 5    995    0.12                          
&#34;        AN       100     3200  5    995    0.36                          
0.20%    SA       200     1600  10   1990   1.29                          
&#34;        ECOLI    100     1600  10   1990   1.34                          
&#34;        PSA      1200    1600  10   1990   1.25                          
&#34;        PC       1800    1250  10   1990   1.60                          
&#34;        CAN      100     15000 10   1990   0.23                          
&#34;        AN       100     3200  10   1990   0.72                          
0.40%    SA       200     1600  20   3980   2.59                          
&#34;        ECOLI    100     1600  20   3980   2.69                          
&#34;        PSA      1200    1600  20   3980   2.50                          
&#34;        PC       1800    1250  20   3980   3.20                          
&#34;        CAN      100     15000 20   3980   0.47                          
&#34;        AN       100     3200  20   3980   1.44                          
0.50%    SA       200     1600  25   4975   3.23                          
&#34;        ECOLI    100     1600  25   4975   3.36                          
&#34;        PSA      1200    1600  25   4975   3.13                          
&#34;        PC       1800    1250  25   4975   3.99                          
&#34;        CAN      100     15000 25   4975   0.58                          
&#34;        AN       100     3200  25   4975   1.80                          
______________________________________                                    
 
    
     
                       TABLE 5                                                     
______________________________________                                    
100:1 Wt. Ratio GERMALL ® II/IPBC                                     
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  1    99     0.07                          
&#34;        ECOLI    100     1600  1    99     0.07                          
&#34;        PSA      1200    1600  1    99     0.06                          
&#34;        PC       1800    1250  1    99     0.08                          
&#34;        CAN      100     15000 1    99     0.02                          
&#34;        AN       100     3200  1    99     0.04                          
 0.025%  SA       200     1600  2.5  248    0.17                          
&#34;        ECOLI    100     1600  2.5  248    0.18                          
&#34;        PSA      1200    1600  2.5  248    0.16                          
&#34;        PC       1800    1250  2.5  248    0.20                          
&#34;        CAN      100     15000 2.5  248    0.04                          
&#34;        AN       100     3200  2.5  248    0.10                          
0.05%    SA       200     1600  5    495    0.33                          
&#34;        ECOLI    100     1600  5    495    0.36                          
&#34;        PSA      1200    1600  5    495    0.31                          
&#34;        PC       1800    1250  5    495    0.40                          
&#34;        CAN      100     15000 5    495    0.08                          
&#34;        AN       100     3200  5    495    0.20                          
0.10%    SA       200     1600  10   990    0.67                          
&#34;        ECOLI    100     1600  10   990    0.72                          
&#34;        PSA      1200    1600  10   990    0.63                          
&#34;        PC       1800    1250  10   990    0.80                          
&#34;        CAN      100     15000 10   990    0.17                          
&#34;        AN       100     3200  10   990    0.41                          
0.20%    SA       200     1600  20   1980   1.34                          
&#34;        ECOLI    100     1600  20   1980   1.44                          
&#34;        PSA      1200    1600  20   1980   1.25                          
&#34;        PC       1800    1250  20   1980   1.60                          
&#34;        CAN      100     15000 20   1980   0.33                          
&#34;        AN       100     3200  20   1980   0.82                          
0.40%    SA       200     1600  40   3960   2.68                          
&#34;        ECOLI    100     1600  40   3960   2.88                          
&#34;        PSA      1200    1600  40   3960   2.51                          
&#34;        PC       1800    1250  40   3960   3.19                          
&#34;        CAN      100     15000 40   3960   0.66                          
&#34;        AN       100     3200  40   3960   1.64                          
0.50%    SA       200     1600  50   4950   3.34                          
&#34;        ECOLI    100     1600  50   4950   3.59                          
&#34;        PSA      1200    1600  50   4950   3.14                          
&#34;        PC       1800    1250  50   4950   3.99                          
&#34;        CAN      100     15000 50   4950   0.83                          
&#34;        AN       100     3200  50   4950   2.05                          
______________________________________                                    
 
    
     
                       TABLE 6                                                     
______________________________________                                    
50:1 Wt. Ratio GERMALL ® II/IPBC                                      
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  2    98     0.07                          
&#34;        ECOLI    100     1600  2    98     0.08                          
&#34;        PSA      1200    1600  2    98     0.06                          
&#34;        PC       1800    1250  2    98     0.08                          
&#34;        CAN      100     15000 2    98     0.03                          
&#34;        AN       100     3200  2    98     0.05                          
 0.025%  SA       200     1600  5    245    0.18                          
&#34;        ECOLI    100     1600  5    245    0.20                          
&#34;        PSA      1200    1600  5    245    0.16                          
&#34;        PC       1800    1250  5    245    0.20                          
&#34;        CAN      100     15000 5    245    0.07                          
&#34;        AN       100     3200  5    245    0.13                          
0.05%    SA       200     1600  10   490    0.36                          
&#34;        ECOLI    100     1600  10   490    0.41                          
&#34;        PSA      1200    1600  10   490    0.31                          
&#34;        PC       1800    1250  10   490    0.40                          
&#34;        CAN      100     15000 10   490    0.13                          
&#34;        AN       100     3200  10   490    0.25                          
0.10%    SA       200     1600  20   980    0.71                          
&#34;        ECOLI    100     1600  20   980    0.81                          
&#34;        PSA      1200    1600  20   980    0.63                          
&#34;        PC       1800    1250  20   980    0.80                          
&#34;        CAN      100     15000 20   980    0.27                          
&#34;        AN       100     3200  20   980    0.51                          
0.20%    SA       200     1600  40   1960   1.43                          
&#34;        ECOLI    100     1600  40   1960   1.63                          
&#34;        PSA      1200    1600  40   1960   1.26                          
&#34;        PC       1800    1250  40   1960   1.59                          
&#34;        CAN      100     15000 40   1960   0.53                          
&#34;        AN       100     3200  40   1960   1.01                          
0.40%    SA       200     1600  80   3920   2.85                          
&#34;        ECOLI    100     1600  80   3920   3.25                          
&#34;        PSA      1200    1600  80   3920   2.52                          
&#34;        PC       1800    1250  80   3920   3.18                          
&#34;        CAN      100     15000 80   3920   1.06                          
&#34;        AN       100     3200  80   3920   2.03                          
0.50%    SA       200     1600  100  4900   3.56                          
&#34;        ECOLI    100     1600  100  4900   4.06                          
&#34;        PSA      1200    1600  100  4900   3.15                          
&#34;        PC       1800    1250  100  4900   3.98                          
&#34;        CAN      100     15000 100  4900   1.33                          
&#34;        AN       100     3200  100  4900   2.53                          
______________________________________                                    
 
    
     
                       TABLE 7                                                     
______________________________________                                    
20:1 Wt. Ratio GERMALL ® II/IPBC                                      
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  5    95     0.08                          
&#34;        ECOLI    100     1600  5    95     0.11                          
&#34;        PSA      1200    1600  5    95     0.06                          
&#34;        PC       1800    1250  5    95     0.08                          
&#34;        CAN      100     15000 5    95     0.06                          
&#34;        AN       100     3200  5    95     0.08                          
 0.025%  SA       200     1600  12.5 237.5  0.21                          
&#34;        ECOLI    100     1600  12.5 237.5  0.27                          
&#34;        PSA      1200    1600  12.5 237.5  0.16                          
&#34;        PC       1800    1250  12.5 237.5  0.20                          
&#34;        CAN      100     15000 12.5 237.5  0.14                          
&#34;        AN       100     3200  12.5 237.5  0.20                          
0.05%    SA       200     1600  25   475    0.42                          
&#34;        ECOLI    100     1600  25   475    0.55                          
&#34;        PSA      1200    1600  25   475    0.32                          
&#34;        PC       1800    1250  25   475    0.39                          
&#34;        CAN      100     15000 25   475    0.28                          
&#34;        AN       100     3200  25   475    0.40                          
0.10%    SA       200     1600  50   950    0.84                          
&#34;        ECOLI    100     1600  50   950    1.09                          
&#34;        PSA      1200    1600  50   950    0.64                          
&#34;        PC       1800    1250  50   950    0.79                          
&#34;        CAN      100     15000 50   950    0.56                          
&#34;        AN       100     3200  50   950    0.80                          
0.20%    SA       200     1600  100  1900   1.69                          
&#34;        ECOLI    100     1600  100  1900   2.19                          
&#34;        PSA      1200    1600  100  1900   1.27                          
&#34;        PC       1800    1250  100  1900   1.58                          
&#34;        CAN      100     15000 100  1900   1.13                          
&#34;        AN       100     3200  100  1900   1.59                          
0.40%    SA       200     1600  200  4800   4.00                          
&#34;        ECOLI    100     1600  200  4800   5.00                          
&#34;        PSA      1200    1600  200  4800   3.17                          
&#34;        PC       1800    1250  200  4800   3.95                          
&#34;        CAN      100     15000 200  4800   2.32                          
&#34;        AN       100     3200  200  4800   3.50                          
0.50%    SA       200     1600  250  4750   4.22                          
&#34;        ECOLI    100     1600  250  4750   5.47                          
&#34;        PSA      1200    1600  250  4750   3.18                          
&#34;        PC       1800    1250  250  4750   3.94                          
&#34;        CAN      100     15000 250  4750   2.82                          
&#34;        AN       100     3200  250  4750   3.98                          
______________________________________                                    
 
    
     
                       TABLE 8                                                     
______________________________________                                    
2000:1 Wt. Ratio DMDMH/IPBC                                               
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  0.05 99.95  0.06                          
&#34;        ECOLI    100     800   0.05 99.95  0.13                          
&#34;        PSA      1200    1600  0.05 99.95  0.06                          
&#34;        PC       1800    1600  0.05 99.95  0.06                          
&#34;        CAN      100     16000 0.05 99.95  0.01                          
&#34;        AN       100     16000 0.05 99.95  0.01                          
 0.025%  SA       200     1600  0.125                                     
                                     249.88 0.16                          
&#34;        ECOLI    100     800   0.125                                     
                                     249.88 0.31                          
&#34;        PSA      1200    1600  0.125                                     
                                     249.88 0.16                          
&#34;        PC       1800    1600  0.125                                     
                                     249.88 0.16                          
&#34;        CAN      100     16000 0.125                                     
                                     249.88 0.02                          
&#34;        AN       100     16000 0.125                                     
                                     249.88 0.02                          
0.05%    SA       200     1600  0.25 499.75 0.31                          
&#34;        ECOLI    100     800   0.25 499.75 0.63                          
&#34;        PSA      1200    1600  0.25 499.75 0.31                          
&#34;        PC       1800    1600  0.25 499.75 0.31                          
&#34;        CAN      100     16000 0.25 499.75 0.03                          
&#34;        AN       100     16000 0.25 499.75 0.03                          
0.10%    SA       200     1600  0.5  999.5  0.63                          
&#34;        ECOLI    100     800   0.5  999.5  1.25                          
&#34;        PSA      1200    1600  0.5  999.5  0.63                          
&#34;        PC       1800    1600  0.5  999.5  0.62                          
&#34;        CAN      100     16000 0.5  999.5  0.07                          
&#34;        AN       100     16000 0.5  999.5  0.07                          
0.20%    SA       200     1600  1    1999   1.25                          
&#34;        ECOLI    100     800   1    1999   2.51                          
&#34;        PSA      1200    1600  1    1999   1.25                          
&#34;        PC       1800    1600  1    1999   1.25                          
&#34;        CAN      100     16000 1    1999   0.13                          
&#34;        AN       100     16000 1    1999   0.13                          
0.40%    SA       200     1600  2    3998   2.51                          
&#34;        ECOLI    100     800   2    3998   5.02                          
&#34;        PSA      1200    1600  2    3998   2.50                          
&#34;        PC       1800    1600  2    3998   2.50                          
&#34;        CAN      100     16000 2    3998   0.27                          
&#34;        AN       100     16000 2    3998   0.27                          
0.50%    SA       200     1600  2.5  4997.5 3.14                          
&#34;        ECOLI    100     800   2.5  4997.5 6.27                          
&#34;        PSA      1200    1600  2.5  4997.5 3.13                          
&#34;        PC       1800    1600  2.5  4997.5 3.12                          
&#34;        CAN      100     16000 2.5  4997.5 0.34                          
&#34;        AN       100     16000 2.5  4997.5 0.34                          
______________________________________                                    
 
    
     
                       TABLE 9                                                     
______________________________________                                    
1000:1 Wt. Ratio DMDMH/IPBC                                               
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  0.1  99.9   0.06                          
&#34;        ECOLI    100     800   0.1  99.9   0.13                          
&#34;        PSA      1200    1600  0.1  99.9   0.06                          
&#34;        PC       1800    1600  0.1  99.9   0.06                          
&#34;        CAN      100     16000 0.1  99.9   0.01                          
&#34;        AN       100     16000 0.1  99.9   0.01                          
 0.025%  SA       200     1600  0.25 249.8  0.16                          
&#34;        ECOLI    100     800   0.25 249.8  0.31                          
&#34;        PSA      1200    1600  0.25 249.8  0.16                          
&#34;        PC       1800    1600  0.25 249.8  0.16                          
&#34;        CAN      100     16000 0.25 249.8  0.02                          
&#34;        AN       100     16000 0.25 249.8  0.02                          
0.05%    SA       200     1600  0.5  499.5  0.31                          
&#34;        ECOLI    100     800   0.5  499.5  0.63                          
&#34;        PSA      1200    1600  0.5  499.5  0.31                          
&#34;        PC       1800    1600  0.5  499.5  0.31                          
&#34;        CAN      100     16000 0.5  499.5  0.04                          
&#34;        AN       100     16000 0.5  499.5  0.04                          
0.10%    SA       200     1600  1    999    0.63                          
&#34;        ECOLI    100     800   1    999    1.26                          
&#34;        PSA      1200    1600  1    999    0.63                          
&#34;        PC       1800    1600  1    999    0.62                          
&#34;        CAN      100     16000 1    999    0.07                          
&#34;        AN       100     16000 1    999    0.07                          
0.20%    SA       200     1600  2    1998   1.26                          
&#34;        ECOLI    100     800   2    1998   2.52                          
&#34;        PSA      1200    1600  2    1998   1.25                          
&#34;        PC       1800    1600  2    1998   1.25                          
&#34;        CAN      100     16000 2    1998   0.14                          
&#34;        AN       100     16000 2    1998   0.14                          
0.40%    SA       200     1600  4    3996   2.52                          
&#34;        ECOLI    100     800   4    3996   5.04                          
&#34;        PSA      1200    1600  4    3996   2.50                          
&#34;        PC       1800    1600  4    3996   2.50                          
&#34;        CAN      100     16000 4    3996   0.29                          
&#34;        AN       100     16000 4    3996   0.29                          
0.50%    SA       200     1600  5    4995   3.15                          
&#34;        ECOLI    100     800   5    4995   6.29                          
&#34;        PSA      1200    1600  5    4995   3.13                          
&#34;        PC       1800    1600  5    4995   3.12                          
&#34;        CAN      100     16000 5    4995   0.36                          
&#34;        AN       100     16000 2.5  4997.5 0.36                          
______________________________________                                    
 
    
     
                       TABLE 10                                                    
______________________________________                                    
500:1 Wt. Ratio DMDMH/IPBC                                                
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  0.2  99.8   0.06                          
&#34;        ECOLI    100     800   0.2  99.8   0.13                          
&#34;        PSA      1200    1600  0.2  99.8   0.06                          
&#34;        PC       1800    1600  0.2  99.8   0.06                          
&#34;        CAN      100     16000 0.2  99.8   0.01                          
&#34;        AN       100     16000 0.2  99.8   0.01                          
0.25%    SA       200     1600  0.5  249.5  0.16                          
&#34;        ECOLI    100     800   0.5  249.5  0.32                          
&#34;        PSA      1200    1600  0.5  249.5  0.16                          
&#34;        PC       1800    1600  0.5  249.5  0.16                          
&#34;        CAN      100     16000 0.5  249.5  0.02                          
&#34;        AN       100     16000 0.5  249.5  0.02                          
0.05%    SA       200     1600  1    499    0.32                          
&#34;        ECOLI    100     800   1    499    0.63                          
&#34;        PSA      1200    1600  1    499    0.31                          
&#34;        PC       1800    1600  1    499    0.31                          
&#34;        CAN      100     16000 1    499    0.04                          
&#34;        AN       100     16000 1    499    0.04                          
0.10%    SA       200     1600  2    998    0.63                          
&#34;        ECOLI    100     800   2    998    1.27                          
&#34;        PSA      1200    1600  2    998    0.63                          
&#34;        PC       1800    1600  2    998    0.62                          
&#34;        CAN      100     16000 2    998    0.08                          
&#34;        AN       100     16000 2    998    0.08                          
0.20%    SA       200     1600  4    1996   1.27                          
&#34;        ECOLI    100     800   4    1996   2.54                          
&#34;        PSA      1200    1600  4    1996   1.25                          
&#34;        PC       1800    1600  4    1996   1.25                          
&#34;        CAN      100     16000 4    1996   0.16                          
&#34;        AN       100     16000 4    1996   0.16                          
0.40%    SA       200     1600  8    3992   2.54                          
&#34;        ECOLI    100     800   8    3992   5.07                          
&#34;        PSA      1200    1600  8    3992   2.50                          
&#34;        PC       1800    1600  8    3992   2.50                          
&#34;        CAN      100     16000 8    3992   0.33                          
&#34;        AN       100     16000 8    3992   0.33                          
0.50%    SA       200     1600  10   4900   3.11                          
&#34;        ECOLI    100     800   10   4900   6.23                          
&#34;        PSA      1200    1600  10   4900   3.07                          
&#34;        PC       1800    1600  10   4900   3.07                          
&#34;        CAN      100     16000 10   4900   0.41                          
&#34;        AN       100     16000 10   4900   0.41                          
0.20%    SA       200     1600  4    1996   1.27                          
&#34;        ECOLI    100     800   4    1996   2.54                          
&#34;        PSA      1200    1600  4    1996   1.25                          
&#34;        PC       1800    1600  4    1996   1.25                          
&#34;        CAN      100     16000 4    1996   0.16                          
&#34;        AN       100     16000 4    1996   0.16                          
0.40%    SA       200     1600  8    3992   2.54                          
&#34;        ECOLI    100     800   8    3992   5.07                          
&#34;        PSA      1200    1600  8    3992   2.50                          
&#34;        PC       1800    1600  8    3992   2.50                          
&#34;        CAN      100     16000 8    3992   0.33                          
&#34;        AN       100     16000 8    3992   0.33                          
0.50%    SA       200     1600  10   4900   3.11                          
&#34;        ECOLI    100     800   10   4900   6.23                          
&#34;        PSA      1200    1600  10   4900   3.07                          
&#34;        PC       1800    1600  10   4900   3.07                          
&#34;        CAN      100     16000 10   4900   0.41                          
&#34;        AN       100     16000 10   4900   0.41                          
______________________________________                                    
 
    
     
                       TABLE 11                                                    
______________________________________                                    
200:1 Wt. Ratio DMDMH/IPBC                                                
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  0.5  99.5   0.06                          
&#34;        ECOLI    100     800   0.5  99.5   0.13                          
&#34;        PSA      1200    1600  0.5  99.5   0.06                          
&#34;        PC       1800    1600  0.5  99.5   0.06                          
&#34;        CAN      100     16000 0.5  99.5   0.01                          
&#34;        AN       100     16000 0.5  99.5   0.01                          
 0.025%  SA       200     1600  1.25 248.75 0.16                          
&#34;        ECOLI    100     800   1.25 248.75 0.32                          
&#34;        PSA      1200    1600  1.25 248.75 0.16                          
&#34;        PC       1800    1600  1.25 248.75 0.16                          
&#34;        CAN      100     16000 1.25 248.75 0.03                          
&#34;        AN       100     16000 1.25 248.75 0.03                          
0.05%    SA       200     1600  2.5  497.5  0.32                          
&#34;        ECOLI    100     800   2.5  497.5  0.65                          
&#34;        PSA      1200    1600  2.5  497.5  0.31                          
&#34;        PC       1800    1600  2.5  497.5  0.31                          
&#34;        CAN      100     16000 2.5  497.5  0.06                          
&#34;        AN       100     16000 2.5  497.5  0.06                          
0.10%    SA       200     1600  5    995    0.65                          
&#34;        ECOLI    100     800   5    995    1.29                          
&#34;        PSA      1200    1600  5    995    0.63                          
&#34;        PC       1800    1600  5    995    0.62                          
&#34;        CAN      100     16000 5    995    0.11                          
&#34;        AN       100     16000 5    995    0.11                          
0.20%    SA       200     1600  10   1990   1.29                          
&#34;        ECOLI    100     800   10   1990   2.59                          
&#34;        PSA      1200    1600  10   1990   1.25                          
&#34;        PC       1800    1600  10   1990   1.25                          
&#34;        CAN      100     16000 10   1990   0.22                          
&#34;        AN       100     16000 10   1990   0.22                          
0.40%    SA       200     1600  20   3980   2.59                          
&#34;        ECOLI    100     800   20   3980   5.18                          
&#34;        PSA      1200    1600  20   3980   2.50                          
&#34;        PC       1800    1600  20   3980   2.50                          
&#34;        CAN      100     16000 20   3980   0.45                          
&#34;        AN       100     16000 20   3980   0.45                          
0.50%    SA       200     1600  25   4975   3.23                          
&#34;        ECOLI    100     800   25   4975   6.47                          
&#34;        PSA      1200    1600  25   4975   3.13                          
&#34;        PC       1800    1600  25   4975   3.12                          
&#34;        CAN      100     16000 25   4975   0.56                          
&#34;        AN       100     16000 25   4975   0.56                          
______________________________________                                    
 
    
     
                       TABLE 12                                                    
______________________________________                                    
100:1 Wt. Ratio DMDMH/IPBC                                                
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  1    99     0.07                          
&#34;        ECOLI    100     800   1    99     0.13                          
&#34;        PSA      1200    1600  1    99     0.06                          
&#34;        PC       1800    1600  1    99     0.06                          
&#34;        CAN      100     16000 1    99     0.02                          
&#34;        AN       100     16000 1    99     0.02                          
 0.025%  SA       200     1600  2.5  248    0.17                          
&#34;        ECOLI    100     800   2.5  248    0.33                          
&#34;        PSA      1200    1600  2.5  248    0.16                          
&#34;        PC       1800    1600  2.5  248    0.16                          
&#34;        CAN      100     16000 2.5  248    0.04                          
&#34;        AN       100     16000 2.5  248    0.04                          
0.05%    SA       200     1600  5    495    0.33                          
&#34;        ECOLI    100     800   5    495    0.67                          
&#34;        PSA      1200    1600  5    495    0.31                          
&#34;        PC       1800    1600  5    495    0.31                          
&#34;        CAN      100     16000 5    495    0.08                          
&#34;        AN       100     16000 5    495    0.08                          
0.10%    SA       200     1600  10   990    0.67                          
&#34;        ECOLI    100     800   10   990    1.34                          
&#34;        PSA      1200    1600  10   990    0.63                          
&#34;        PC       1800    1600  10   990    0.62                          
&#34;        CAN      100     16000 10   990    0.16                          
&#34;        AN       100     16000 10   990    0.16                          
0.20%    SA       200     1600  20   1980   1.34                          
&#34;        ECOLI    100     800   20   1980   2.68                          
&#34;        PSA      1200    1600  20   1980   1.25                          
&#34;        PC       1800    1600  20   1980   1.25                          
&#34;        CAN      100     16000 20   1980   0.32                          
&#34;        AN       100     16000 20   1980   0.32                          
0.40%    SA       200     1600  40   3960   2.68                          
&#34;        ECOLI    100     800   40   3960   5.35                          
&#34;        PSA      1200    1600  40   3960   2.51                          
&#34;        PC       1800    1600  40   3960   2.50                          
&#34;        CAN      100     16000 40   3960   0.65                          
&#34;        AN       100     16000 40   3960   0.65                          
0.50%    SA       200     1600  50   4950   3.34                          
&#34;        ECOLI    100     800   50   4950   6.69                          
&#34;        PSA      1200    1600  50   4950   3.14                          
&#34;        PC       1800    1600  50   4950   3.12                          
&#34;        CAN      100     16000 50   4950   0.81                          
&#34;        AN       100     16000 50   4950   0.81                          
______________________________________                                    
 
    
     
                       TABLE 13                                                    
______________________________________                                    
50:1 Wt. Ratio DMDMH/IPBC                                                 
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  2    98     0.07                          
&#34;        ECOLI    100     800   2    98     0.14                          
&#34;        PSA      1200    1600  2    98     0.06                          
&#34;        PC       1800    1600  2    98     0.06                          
&#34;        CAN      100     16000 2    98     0.03                          
&#34;        AN       100     16000 2    98     0.03                          
 0.025%  SA       200     1600  5    245    0.18                          
&#34;        ECOLI    100     800   5    245    0.36                          
&#34;        PSA      1200    1600  5    245    0.16                          
&#34;        PC       1800    1600  5    245    0.16                          
&#34;        CAN      100     16000 5    245    0.07                          
&#34;        AN       100     16000 5    245    0.07                          
0.05%    SA       200     1600  10   490    0.36                          
&#34;        ECOLI    100     800   10   490    0.71                          
&#34;        PSA      1200    1600  10   490    0.31                          
&#34;        PC       1800    1600  10   490    0.31                          
&#34;        CAN      100     16000 10   490    0.13                          
&#34;        AN       100     16000 10   490    0.13                          
0.10%    SA       200     1600  20   980    0.71                          
&#34;        ECOLI    100     800   20   980    1.43                          
&#34;        PSA      1200    1600  20   980    0.63                          
&#34;        PC       1800    1600  20   980    0.62                          
&#34;        CAN      100     16000 20   980    0.26                          
&#34;        AN       100     16000 20   980    0.26                          
0.20%    SA       200     1600  40   1960   1.43                          
&#34;        ECOLI    100     800   40   1960   2.85                          
&#34;        PSA      1200    1600  40   1960   1.26                          
&#34;        PC       1800    1600  40   1960   1.25                          
&#34;        CAN      100     16000 40   1960   0.52                          
&#34;        AN       100     16000 40   1960   0.52                          
0.40%    SA       200     1600  80   3920   2.85                          
&#34;        ECOLI    100     800   80   3920   5.70                          
&#34;        PSA      1200    1600  80   3920   2.52                          
&#34;        PC       1800    1600  80   3920   2.49                          
&#34;        CAN      100     16000 80   3920   1.05                          
&#34;        AN       100     16000 80   3920   1.05                          
0.50%    SA       200     1600  100  4900   3.56                          
&#34;        ECOLI    100     800   100  4900   7.13                          
&#34;        PSA      1200    1600  100  4900   3.15                          
&#34;        PC       1800    1600  100  4900   3.12                          
&#34;        CAN      100     16000 100  4900   1.31                          
&#34;        AN       100     16000 100  4900   1.31                          
______________________________________                                    
 
    
     
                       TABLE 14                                                    
______________________________________                                    
20:1 Wt. Ratio DMDMH/IPBC                                                 
Use Level                                                                 
         Organism Q.sub.a Q.sub.b                                         
                                Q.sub.A                                   
                                     Q.sub.B                              
                                            SI                            
______________________________________                                    
0.01%    SA       200     1600  5    95     0.08                          
&#34;        ECOLI    100     800   5    95     0.17                          
&#34;        PSA      1200    1600  5    95     0.06                          
&#34;        PC       1800    1600  5    95     0.06                          
&#34;        CAN      100     16000 5    95     0.06                          
&#34;        AN       100     16000 5    95     0.06                          
 0.025%  SA       200     1600  12.5 237.5  0.21                          
&#34;        ECOLI    100     800   12.5 237.5  0.42                          
&#34;        PSA      1200    1600  12.5 237.5  0.16                          
&#34;        PC       1800    1600  12.5 237.5  0.16                          
&#34;        CAN      100     16000 12.5 237.5  0.14                          
&#34;        AN       100     16000 12.5 237.5  0.14                          
0.05%    SA       200     1600  25   475    0.42                          
&#34;        ECOLI    100     800   25   475    0.84                          
&#34;        PSA      1200    1600  25   475    0.32                          
&#34;        PC       1800    1600  25   475    0.31                          
&#34;        CAN      100     16000 25   475    0.28                          
&#34;        AN       100     16000 25   475    0.28                          
0.10%    SA       200     1600  50   950    0.84                          
&#34;        ECOLI    100     800   50   950    1.69                          
&#34;        PSA      1200    1600  50   950    0.64                          
&#34;        PC       1800    1600  50   950    0.62                          
&#34;        CAN      100     16000 50   950    0.56                          
&#34;        AN       100     16000 50   950    0.56                          
0.20%    SA       200     1600  100  1900   1.69                          
&#34;        ECOLI    100     800   100  1900   3.38                          
&#34;        PSA      1200    1600  100  1900   1.27                          
&#34;        PC       1800    1600  100  1900   1.24                          
&#34;        CAN      100     16000 100  1900   1.12                          
&#34;        AN       100     16000 100  1900   1.12                          
0.40%    SA       200     1600  200  4800   4.00                          
&#34;        ECOLI    100     800   200  4800   8.00                          
&#34;        PSA      1200    1600  200  4800   3.17                          
&#34;        PC       1800    1600  200  4800   3.11                          
&#34;        CAN      100     16000 200  4800   2.30                          
&#34;        AN       100     16000 200  4800   2.30                          
0.50%    SA       200     1600  250  4750   4.22                          
&#34;        ECOLI    100     800   250  4750   8.44                          
&#34;        PSA      1200    1600  250  4750   3.18                          
&#34;        PC       1800    1600  250  4750   3.11                          
&#34;        CAN      100     16000 250  4750   2.80                          
&#34;        AN       100     16000 250  4750   2.80                          
______________________________________                                    
 
    
     Similar SI results also were found with GADM and SUTTOCIDE® A as the methylol compound in place of Germall® II or DMDMH in admixtures with IPBC over the same wt. ratios and use level ranges as shown in the Tables 1-14 above. 
     Tables 1 through 14 above illustrate the synergism of IPBC (compound B) with Germall® II or DMDMH (compound A) at weight ratios of A:B of 2000:1, 1000:1, 500:1, 200:1, 100:1, 50:1 and 20:1. Synergism is very effective for all ratios at low use levels, e.g. 0.01% to 0.1%, against all tested gram-positive, gram-negative organisms and fungi organisms. At slightly higher use concentrations, e.g. 0.20 to 0.50%, all tested ratios were synergistic against Candida albicans and A. niger also. However, at 50:1 and 20:1 ratios, the synergistic effect is negligible at the 0.01 to 0.1% use levels, and non-synergistic even against Candida albicans and A. niger at use levels of 0.20 to 0.50%. 
     The SI values were lower for Germall® II as the methylol compound in the admixtures as compared to DMDMH. 
     Similar results were obtained when iodopropynyl alcohol (IPGA) was substituted for IPBC in the admixtures described above. 
     3. PRESERVATIVE ACTIVITY (CHALLENGE TEST) 
     A typical cosmetic emulsion was prepared for microbiological challenge testing and predetermined admixtures of a methylol compound and IPBC were added at various use levels. The emulsion thus prepared had the following composition: 
     
         ______________________________________                                    
                          % wt.                                           
______________________________________                                    
Phase A                                                                   
Stearic Acid                5.00                                          
Mineral Oil                 2.50                                          
Cetyl Alcohol               1.00                                          
Lareth-5 and Ceteth-5 and Oleth-5 and Steareth-5                          
                            0.50                                          
Glycerol Monostearate and Polyoxyethylene Stearate                        
                            1.50                                          
Phase B                                                                   
Deionized Water             88.0                                          
Triethanolamine 99%         1.00                                          
Citric Acid 30% aqueous solution                                          
                            0.60                                          
Preservative Admixture      qs                                            
______________________________________                                    
 
    
     To prepare the emulsion, Phases A and B were heated separately to 75°-80° C. Phase A then was added to Phase B with mixing. The mixture then was cooled to 55°-60° C. At this point the desired amount of the preservative admixture was added and the product was cooled to 50° C. while stirring. The citric acid solution then was added to adjust the pH and the mixture was stirred until a temperature of 30° C. was reached. 
     The challenge tests were carried out using the following microorganisms: SA, ECOLI, PSA, PC, AN and CAN, in this manner. 50 g. aliquots of the test emulsion containing various amounts of the preservative admixture were inoculated with approximately 10 7  -10 8  of the challenge organisms. The test samples then were stirred to disperse the challenge inoculum. The samples were incubated and assayed at 48 hours, 7, 14, 21 and 28 days. The assays were performed on 1 g. of the test sample by serially diluting 10 1  to 10 6  of the original concentration. The plating medium for bacteria was Letheen agar and for fungi it was low pH Mycophil agar with Tween 20. Each plated sample was incubated for 48 hours at 37° C. for bacteria, 5 days at 25° C. for mold, and 3 days at 25° C. for fungi. After incubation, readings of the number of colonies per milliliter (cfu/ml) were made. At 21 days the test product was reinoculated with half of the original inoculum. The data is presented in Tables 15-23 below. 
     
                       TABLE 15                                                    
______________________________________                                    
2000:1 GERMALL ® II/IPBC                                              
                 48      7     14    21    28                             
Test Level                                                                
        Organism Hours   Days  Days  Days  Days                           
______________________________________                                    
 0.01%  AN       69,000  260,000                                          
                               190,000                                    
                                     17,000                               
                                           4,500                          
&#34;       CAN      98,000  76,000                                           
                               1,400 3,100 19,000                         
&#34;       ECOLI    110,000 290,000                                          
                               2,400 138,000                              
                                           560,000                        
&#34;       PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       SA       190,000 220   &lt;10   &lt;10   3,700                          
 0.025% AN       2,800   10    10    &lt;10   220                            
&#34;       CAN      58,000  29,000                                           
                               18,000                                     
                                     56,000                               
                                           110,000                        
&#34;       ECOLI    39,000  10    &lt;10   &lt;10   &lt;10                            
&#34;       PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       SA       37,000  170   &lt;10   &lt;10   &lt;10                            
 0.05%  AN       20      &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       CAN      19,000  6,600 70    &lt;10   320                            
&#34;       ECOLI    3,400   &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       SA       31,000  &lt;10   &lt;10   &lt;10   &lt;10                            
0.1%    AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       CAN      180     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       ECOLI    &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       SA       750     &lt;10   &lt;10   &lt;10   &lt;10                            
0.2%    AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       CAN      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       ECOLI    &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       SA       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
______________________________________                                    
Unpreserved control                                                       
Organism                                                                  
        48 Hours 7 Days   14 Days                                         
                                 21 Days                                  
                                        28 Days                           
______________________________________                                    
AN      52,000   27,000   19,000 19,000 19,000                            
CAN     110,000  130,000  240,000                                         
                                 180,000                                  
                                        240,000                           
ECOLI   54,000   140,000  170,000                                         
                                 170,000                                  
                                        74,000                            
PC      6,400,000                                                         
                 6,400,000                                                
                          2,000,000                                       
                                 6,700,000                                
                                        29,000                            
PSA     110,000  700      110,000                                         
                                 290,000                                  
                                        85,000                            
SA      2,800,000                                                         
                 250,000  51,000 3,700  330                               
______________________________________                                    
Inoculum Concentration                                                    
Organism       0 Hours  21 Days                                           
______________________________________                                    
AN             26,000   53,000                                            
CAN            1,000,000                                                  
                        1,900,000                                         
ECOLI          3,600,000                                                  
                        170,000                                           
PC             3,400,000                                                  
                        87,000                                            
PSA            4,500,000                                                  
                        390,000                                           
SA             4,100,000                                                  
                        200,000                                           
______________________________________                                    
 
    
     
                       TABLE 16                                                    
______________________________________                                    
1000:1 GERMALL ® II/IPBC                                              
Test           48      7     14    21    28                               
Level Organism Hours   Days  Days  Days  Days                             
______________________________________                                    
0.01% AN       34,000  3,500 80    10    &lt;10                              
&#34;     CAN      420,000 24,000                                             
                             950   6,400 6,400,000                        
&#34;     ECOLI    120,000 63,000                                             
                             93,000                                       
                                   92,000                                 
                                         9,600,000                        
&#34;     PC       10      25,000                                             
                             1,600 15,800                                 
                                         33,000,000                       
&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     SA       100,000 1,400 &lt;10   &lt;10   5,000                            
0.025%                                                                    
      AN       530     10    &lt;10   &lt;10   &lt;10                              
&#34;     CAN      34,000  750   10    770   240,000                          
&#34;     ECOLI    120,000 &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     SA       37,000  170   &lt;10   &lt;10   &lt;10                              
0.05% AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     CAN      13,000  &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     ECOLI    68,000  &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     SA       21,000  &lt;10   &lt;10   &lt;10   &lt;10                              
0.1%  AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     CAN      10      &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     ECOLI    &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     SA       1,400   &lt;10   &lt;10   &lt;10   &lt;10                              
0.2%  AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     CAN      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     ECOLI    &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
&#34;     SA       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                              
______________________________________                                    
Unpreserved control                                                       
Organism                                                                  
        48 Hours 7 Days   14 Days                                         
                                 21 Days                                  
                                        28 Days                           
______________________________________                                    
AN      52,000   27,000   19,000 19,000 19,000                            
CAN     110,000  130,000  240,000                                         
                                 180,000                                  
                                        240,000                           
ECOLI   54,000   140,000  170,000                                         
                                 170,000                                  
                                        74,000                            
PC      6,400,000                                                         
                 6,400,000                                                
                          2,000,000                                       
                                 6,700,000                                
                                        29,000                            
PSA     110,000  700      110,000                                         
                                 290,000                                  
                                        85,000                            
SA      2,800,000                                                         
                 250,000  51,000 3,700  330                               
______________________________________                                    
Inoculum Concentration                                                    
Organism       0 Hours  21 Days                                           
______________________________________                                    
AN             26,000   53,000                                            
CAN            1,000,000                                                  
                        1,900,000                                         
ECOLI          3,600,000                                                  
                        170,000                                           
PC             3,400,000                                                  
                        87,000                                            
PSA            4,500,000                                                  
                        390,000                                           
SA             4,100,000                                                  
                        200,000                                           
______________________________________                                    
 
    
     
                                           TABLE 17                                
__________________________________________________________________________
500:1 GERMALL ® II/IPBC                                               
Test Level                                                                
      Organism                                                            
            48 Hours                                                      
                 7 Days                                                   
                      14 Days                                             
                           21 Days                                        
                                 28 Days                                  
__________________________________________________________________________
 0.01%                                                                    
      AN    23,000                                                        
                 40   &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   170,000                                                       
                 5,600                                                    
                      290  200   &lt;10                                      
&#34;     ECOLI 90,000                                                        
                 57,000                                                   
                      95,000                                              
                           70,000                                         
                                 240,000                                  
&#34;     PC    10   &lt;10  &lt;10  &gt;10,000                                        
                                 42,000,000                               
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    380,000                                                       
                 440  &lt;10  &lt;10   5,100                                    
 0.05%                                                                    
      AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   8,700                                                         
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI 60,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    31,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.1%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    890  &lt;10  &lt;10  &lt;10   &lt;10                                      
0.2%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
__________________________________________________________________________
Unpreserved control                                                       
Organism                                                                  
     48 Hours                                                             
             7 Days                                                       
                   14 Days                                                
                          21 Days                                         
                               28 Days                                    
__________________________________________________________________________
AN   6,100   520,000                                                      
                   18,000 5,000                                           
                               11,000                                     
CAN  1,000,000                                                            
             710,000                                                      
                   95,000 12,000                                          
                               64,000                                     
ECOLI                                                                     
     7,100,000                                                            
             6,200,000                                                    
                   610,000                                                
                          350,000                                         
                               120,000                                    
PC   14,600,000                                                           
             160,000,000                                                  
                   3,600,000                                              
                          2,720,000                                       
                               9,500,000                                  
PSA  20      900   130    4,100                                           
                               &gt;100,000                                   
SA   43,000,000                                                           
             600,000                                                      
                   1,000  220  &lt;10                                        
__________________________________________________________________________
Inoculum Concentration                                                    
Organism     0 Hours                                                      
                  21 Days                                                 
__________________________________________________________________________
AN           19,000                                                       
                  4,700,000                                               
CAN          340,000                                                      
                  16,000,000                                              
ECOLI        3,900,000                                                    
                  1,480,000                                               
PC           3,800,000                                                    
                  1,380,000                                               
PSA          9,200,000                                                    
                  730,000                                                 
SA           4,800,000                                                    
                  360,000                                                 
__________________________________________________________________________
 
    
     
                                           TABLE 18                                
__________________________________________________________________________
200:1 GERMALL ® II/IPBC                                               
Test Level                                                                
      Organism                                                            
            48 Hours                                                      
                 7 Days                                                   
                      14 Days                                             
                           21 Days                                        
                                 28 Days                                  
__________________________________________________________________________
 0.01%                                                                    
      AN    47,000                                                        
                 320  10   &lt;10   &lt;10                                      
&#34;     CAN   810,000                                                       
                 450,000                                                  
                      410,000                                             
                           190,000                                        
                                 63,000                                   
&#34;     ECOLI 220,000                                                       
                 7,600                                                    
                      &lt;10  850   &gt;1,000,000                               
&#34;     PC    10,000                                                        
                 500,000                                                  
                      1,900,000                                           
                           1,100,000                                      
                                 193,000                                  
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    190,000                                                       
                 23,000                                                   
                      120  &lt;10   &lt;10                                      
 0.05%                                                                    
      AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   190  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI 37,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    19,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.1%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   10   &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    45,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.2%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
__________________________________________________________________________
Unpreserved control                                                       
Organism                                                                  
     48 Hours                                                             
             7 Days                                                       
                   14 Days                                                
                          21 Days                                         
                               28 Days                                    
__________________________________________________________________________
AN   89,000  32,000                                                       
                   22,000 16,000                                          
                               16,000                                     
CAN  210,000 670,000                                                      
                   430,000                                                
                          590,000                                         
                               640,000                                    
ECOLI                                                                     
     640,000 360,000                                                      
                   410,000                                                
                          990,000                                         
                               68,000                                     
PC   19,000,000                                                           
             3,200,000                                                    
                   7,000,000                                              
                          &gt;10,000                                         
                               2,760,000                                  
PSA  80      9,400 200,000                                                
                          &gt;10,000                                         
                               34,000                                     
SA   6,300,000                                                            
             190,000                                                      
                   11,000 580  120                                        
__________________________________________________________________________
Inoculum Concentration                                                    
Organism     0 Hours                                                      
                  21 Days                                                 
__________________________________________________________________________
AN           41,000                                                       
                  32,000                                                  
CAN          640  1,100,000                                               
ECOLI        5,800,000                                                    
                  1,300,000                                               
PC           900,000                                                      
                  3,000,000                                               
PSA          1,800,000                                                    
                  4,900,000                                               
SA           7,200,000                                                    
                  2,000,000                                               
__________________________________________________________________________
 
    
     
                       TABLE 19                                                    
______________________________________                                    
50:1 GERMALL ® II/IPBC                                                
                 48      7     14    21    28                             
Test Level                                                                
        Organism Hours   Days  Days  Days  Days                           
______________________________________                                    
 0.01%  AN       4,100   40    &lt;10   &lt;10   &lt;10                            
&#34;       CAN      310,000 7,700 560   5,600 5,200                          
&#34;       ECOLI    170,000 710   10    &lt;10   120                            
&#34;       PC       7,400   74,000                                           
                               340,000                                    
                                     720,000                              
                                           520,000                        
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   30                             
&#34;       SA       110,000 11,000                                           
                               &lt;10   &lt;10   9,200                          
 0.05%  AN       &lt;10     100   &lt;10   &lt;10   &lt;10                            
&#34;       CAN      210     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       ECOLI    150,000 &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       SA       35,000  &lt;10   &lt;10   &lt;10   &lt;10                            
0.1%    AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       CAN      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       ECOLI    510     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       SA       3,000   &lt;10   &lt;10   &lt;10   &lt;10                            
0.2%    AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       CAN      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       ECOLI    &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
&#34;       SA       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10                            
______________________________________                                    
Unpreserved control                                                       
Organism                                                                  
       48 Hours  7 Days   14 Days                                         
                                 21 Days                                  
                                        28 Days                           
______________________________________                                    
AN     89,000    32,000   22,000 16,000 16,000                            
CAN    210,000   670,000  430,000                                         
                                 590,000                                  
                                        640,000                           
ECOLI  640,000   360,000  410,000                                         
                                 990,000                                  
                                        68,000                            
PC     19,000,000                                                         
                 3,200,000                                                
                          7,000,000                                       
                                 &gt;10,000                                  
                                        2,760,000                         
PSA    80        9,400    200,000                                         
                                 &gt;10,000                                  
                                        34,000                            
SA     6,300,000 190,000  11,000 580    120                               
______________________________________                                    
Inoculum Concentration                                                    
Organism       0 Hours  21 Days                                           
______________________________________                                    
AN             41,000   32,000                                            
CAN            640      1,100,000                                         
ECOLI          5,800,000                                                  
                        1,300,000                                         
PC             900,000  3,000,000                                         
PSA            1,800,000                                                  
                        4,900,000                                         
SA             7,200,000                                                  
                        2,000,000                                         
______________________________________                                    
 
    
     
                                           TABLE 20                                
__________________________________________________________________________
20:1 GERMALL ® II/IPBC                                                
Test Level                                                                
      Organism                                                            
            48 Hours                                                      
                 7 Days                                                   
                      14 Days                                             
                           21 Days                                        
                                 28 Days                                  
__________________________________________________________________________
 0.01%                                                                    
      AN    3,100                                                         
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   75,000                                                        
                 220  &lt;10  &lt;10   2,400                                    
&#34;     ECOLI 160,000                                                       
                 110  &lt;10  &lt;10   20                                       
&#34;     PC    12,000                                                        
                 1,000,000                                                
                      2,100,000                                           
                           &gt;1,000,000                                     
                                 730,000                                  
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   4,000                                    
&#34;     SA    140,000                                                       
                 4,100                                                    
                      &lt;10  &lt;10   1,680                                    
 0.05%                                                                    
      AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI 16,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10                                            
&#34;     SA    31,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.1%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    6,800                                                         
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.2%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
__________________________________________________________________________
Unpreserved control                                                       
Organism                                                                  
     48 Hours                                                             
             7 Days                                                       
                   14 Days                                                
                          21 Days                                         
                               28 Days                                    
__________________________________________________________________________
AN   89,000  32,000                                                       
                   22,000 16,000                                          
                               16,000                                     
CAN  210,000 670,000                                                      
                   430,000                                                
                          590,000                                         
                               640,000                                    
ECOLI                                                                     
     640,000 360,000                                                      
                   410,000                                                
                          990,000                                         
                               68,000                                     
PC   19,000,000                                                           
             3,200,000                                                    
                   7,000,000                                              
                          &gt;10,000                                         
                               2,760,000                                  
PSA  80      9,400 200,000                                                
                          &gt;10,000                                         
                               34,000                                     
SA   6,300,000                                                            
             190,000                                                      
                   11,000 580  120                                        
__________________________________________________________________________
Inoculum Concentration                                                    
Organism     0 Hours                                                      
                  21 Days                                                 
__________________________________________________________________________
AN           41,000                                                       
                  32,000                                                  
CAN          640  1,100,000                                               
ECOLI        5,800,000                                                    
                  1,300,000                                               
PC           900,000                                                      
                  3,000,000                                               
PSA          1,800,000                                                    
                  4,900,000                                               
SA           7,200,000                                                    
                  2,000,000                                               
__________________________________________________________________________
 
    
     
                                           TABLE 21                                
__________________________________________________________________________
2000:1 DMDMH/IPBC                                                         
Test Level                                                                
      Organism                                                            
            48 Hours                                                      
                 7 Days                                                   
                      14 Days                                             
                           21 Days                                        
                                 28 Days                                  
__________________________________________________________________________
 0.025%                                                                   
      AN    4,100                                                         
                 38,000                                                   
                      270  80    100                                      
&#34;     CAN   270,000                                                       
                 1,900,000                                                
                      550,000                                             
                           220,000                                        
                                 210,000                                  
&#34;     ECOLI 1,300,000                                                     
                 &lt;10  &lt;10  &lt;10   3,000                                    
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    42,000                                                        
                 20   &lt;10  &lt;10   &lt;10                                      
 0.05%                                                                    
      AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   770,000                                                       
                 130,000                                                  
                      670,000                                             
                           160,000                                        
                                 64,000                                   
&#34;     ECOLI 220,000                                                       
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    9,000                                                         
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.1%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   580  840  500  40,000                                         
                                 83,000                                   
&#34;     ECOLI 340  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    29,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.2%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   &lt;10  10   10   &lt;10   21                                       
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
__________________________________________________________________________
Unpreserved control                                                       
Organism                                                                  
     48 Hours                                                             
             7 Days                                                       
                   14 Days                                                
                          21 Days                                         
                               28 Days                                    
__________________________________________________________________________
AN   37,000  36,000                                                       
                   24,000 5,200                                           
                               6,000                                      
CAN  120,000 1,900,000                                                    
                   3,300,000                                              
                          480,000                                         
                               790,000                                    
ECOLI                                                                     
     150,000 2,500,000                                                    
                   7,300,000                                              
                          240,000                                         
                               140,000                                    
PC   19,000,000                                                           
             15,600,000                                                   
                   5,900,000                                              
                          8,500,000                                       
                               31,000,000                                 
PSA  &lt;10     &lt;10   100    15,200                                          
                               300,000                                    
SA   7,000,000                                                            
             &gt;1,000,000                                                   
                   12,000 3,000                                           
                               110                                        
__________________________________________________________________________
Inoculum Concentration                                                    
Organism     0 Hours                                                      
                  21 Days                                                 
__________________________________________________________________________
AN           50,000                                                       
                  41,000                                                  
CAN          1,400,000                                                    
                  640                                                     
ECOLI        4,800,000                                                    
                  5,800,000                                               
PC           9,200,000                                                    
                  900,000                                                 
PSA          6,900,000                                                    
                  9,000,000                                               
SA           5,700,000                                                    
                  7,200,000                                               
__________________________________________________________________________
 
    
     
                                           TABLE 22                                
__________________________________________________________________________
1000:1 DMDMH/IPBC                                                         
Test Level                                                                
      Organism                                                            
            48 Hours                                                      
                 7 Days                                                   
                      14 Days                                             
                           21 Days                                        
                                 28 Days                                  
__________________________________________________________________________
 0.025%                                                                   
      AN    650  900  &lt;10  &lt;10   140                                      
&#34;     CAN   97,000                                                        
                 900,000                                                  
                      2,300,000                                           
                           120,000                                        
                                 120,000                                  
&#34;     ECOLI 160,000                                                       
                 &lt;10  &lt;10  &lt;10   2,400                                    
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    23,000                                                        
                 &lt;10  &lt;10  &lt;10   90                                       
 0.05%                                                                    
      AN    20   &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   65,000                                                        
                 520,000                                                  
                      56,000                                              
                           32,000                                         
                                 370,000                                  
&#34;     ECOLI 26,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    12,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.1%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   3,100                                                         
                 4,800                                                    
                      180  640   4,400                                    
&#34;     ECOLI 45,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    6,000                                                         
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.2%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    180  &lt;10  &lt;10  &lt;10   &lt;10                                      
__________________________________________________________________________
Unpreserved control                                                       
Organism                                                                  
     48 Hours                                                             
             7 Days                                                       
                   14 Days                                                
                          21 Days                                         
                               28 Days                                    
__________________________________________________________________________
AN   37,000  36,000                                                       
                   24,000 5,200                                           
                               6,000                                      
CAN  120,000 1,900,000                                                    
                   3,300,000                                              
                          480,000                                         
                               790,000                                    
ECOLI                                                                     
     150,000 2,500,000                                                    
                   7,300,000                                              
                          240,000                                         
                               140,000                                    
PC   19,000,000                                                           
             15,600,000                                                   
                   5,900,000                                              
                          8,500,000                                       
                               31,000,000                                 
PSA  &lt;10     &lt;10   100    15,200                                          
                               300,000                                    
SA   7,000,000                                                            
             &gt;1,000,000                                                   
                   12,000 3,000                                           
                               110                                        
__________________________________________________________________________
Inoculum Concentration                                                    
Organism     0 Hours                                                      
                  21 Days                                                 
__________________________________________________________________________
AN           50,000                                                       
                  41,000                                                  
CAN          1,400,000                                                    
                  640                                                     
ECOLI        4,800,000                                                    
                  5,800,000                                               
PC           9,200,000                                                    
                  900,000                                                 
PSA          6,900,000                                                    
                  9,000,000                                               
SA           5,700,000                                                    
                  7,200,000                                               
__________________________________________________________________________
 
    
     
                                           TABLE 23                                
__________________________________________________________________________
2000:1 GADM/IPBC                                                          
Test Level                                                                
      Organism                                                            
            48 Hours                                                      
                 7 Days                                                   
                      14 Days                                             
                           21 Days                                        
                                 28 Days                                  
__________________________________________________________________________
 0.025%                                                                   
      AN    300  21   10   &lt;10   11                                       
&#34;     CAN   480,000                                                       
                 890,000                                                  
                      940,000                                             
                           1,040,000                                      
                                 130,000                                  
&#34;     ECOLI 230,000                                                       
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    78,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
 0.05%                                                                    
      AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   110,000                                                       
                 4,100,000                                                
                      3,600,000                                           
                           330,000                                        
                                 97,000                                   
&#34;     ECOLI 120,000                                                       
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    56,000                                                        
                 &lt;10  &lt;10  &lt;10   &lt;10                                      
0.1%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   1,200,000                                                     
                 53,000                                                   
                      430,000                                             
                           144,000                                        
                                 110,000                                  
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
0.2%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     CAN   90,000                                                        
                 &lt;10  &lt;10  &lt;10   60                                       
&#34;     ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
&#34;     SA    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10                                      
__________________________________________________________________________
Unpreserved control                                                       
Organism                                                                  
     48 Hours                                                             
             7 Days                                                       
                   14 Days                                                
                          21 Days                                         
                                28 Days                                   
__________________________________________________________________________
AN   50,000  33,000                                                       
                   33,000 13,000                                          
                                5,400                                     
CAN  780,000 780,000                                                      
                   780,000                                                
                          500,000                                         
                                170,000                                   
ECOLI                                                                     
     600,000 3,100,000                                                    
                   920,000                                                
                          920,000                                         
                                140,000                                   
PC   11,000,000                                                           
             30,000,000                                                   
                   10,000,000                                             
                          10,000,000                                      
                                1,400,000                                 
PSA  3,800   600   12,800 12,800                                          
                                100,000                                   
SA   14,000,000                                                           
             410,000                                                      
                   7,100  7,100 80                                        
__________________________________________________________________________
Inoculum Concentration                                                    
Organism     0 Hours                                                      
                  21 Days                                                 
__________________________________________________________________________
AN           53,000                                                       
                  10,000                                                  
CAN          1,900,000                                                    
                  310,000                                                 
ECOLI        170,000                                                      
                  3,500,000                                               
PC           87,000                                                       
                  2,500,000                                               
PSA          390,000                                                      
                  5,400,000                                               
SA           200,000                                                      
                  4,100,000                                               
__________________________________________________________________________
 
    
     Discussion of Challenge Testing Results 
     The 28-day challenge results reported in Tables 15-23 above demonstrate the effectiveness of the preservative admixture of the invention in a use emulsion composition against a wide range of bacteria and fungi organisms. 
     For example, admixture compositions of Germall® II and IPBC at a wt. ratio of 2000:1 (Table 15), when present at use levels of 0.05 to 0.2%, corresponding to 0.75 to 10 ppm IPBC and 500 to 2000 ppm methylol levels, provide substantially complete protection against all tested organisms after 28 days. At the low use level of 0.05% active, all the challenge tests passed within 21 days. Then, upon reinoculation after 21 days, all organisms died within 7 days except CAN which cleared within 14 days. 
     Table 21 shows the challenge test results for DMDMH and IPBC admixtures at the same 2000:1 wt. ratio. A use level of 0.2%, however, is needed for this blend to pass against all organisms after 21 days. Upon reinoculation, all organisms died within 7 days with the exception of CAN which cleared within 14 days. It is thus evident from these results that Germall® II blended with IPBC is 4 times more effective than a DMDMH/IPBC blend. 
     Germall® II also is superior to GADM as the methylol compound, as shown in Table 23. 
     Table 24 below is a study of the activity of solution of GII/IPBC in propylene glycol. The admixtures of the active GII and IPBC components were prepared at weight ratios of 99.5%/0.5% and 99%/1%, and added to 60% by weight propylene glycol. The resulting solutions were tested at 0.05%, 0.1% and 0.2% total active in a proteinaceous shampoo formulation. 
     
                                           TABLE 24                                
__________________________________________________________________________
Test Level                                                                
      Organism                                                            
           48 Hours                                                       
                7 Days                                                    
                      14 Days                                             
                           21 Days                                        
                                  28 Days                                 
__________________________________________________________________________
99.5% GII/0.5% IPBC/propylene glycol solution - 0.05% total active        
 0.05%                                                                    
      AN   310,000                                                        
                2,000 &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  44,000                                                         
                2,400 &lt;10  &lt;10    1,800                                   
&#34;     ECOLI                                                               
           3,000                                                          
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   400,000                                                        
                62,000                                                    
                      90   &lt;10    &gt;10,000                                 
&#34;     PSA  5,300,000                                                      
                3,000 &lt;10  &lt;10    &gt;10,000                                 
&#34;     SA   10   10    &lt;10  &lt;10    &lt;10                                     
99.5% GII/0.5% IPBC/propylene glycol solution - 0.1% total active         
0.1%  AN   210,000                                                        
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  1,500                                                          
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           580  &lt;10   &lt;10  &lt;10    20                                      
&#34;     PC   34,000                                                         
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  780  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
99.5% GII/0.5% IPBC/propylene glycol solution - 0.2% total active         
0.2%  AN   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
99% GII/1% IPBC/propylene glycol solution - 0.05% total active            
 0.05%                                                                    
      AN   89,000                                                         
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  4,400                                                          
                &lt;10   &lt;10  &lt;10    20                                      
&#34;     ECOLI                                                               
           3,300                                                          
                &lt;10   &lt;10  &lt;10    60                                      
&#34;     PC   260,000                                                        
                &lt;10   &lt;10  &lt;10    3,400                                   
&#34;     PSA  64,000                                                         
                &lt;10   &lt;10  &lt;10    280                                     
&#34;     SA   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
99% GII/1% IPBC/propylene glycol solution - 0.1% total active             
0.1%  AN   37,000                                                         
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           840  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   48,000                                                         
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  440  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
99% GII/1% IPBC/propylene glycol solution - 0.2% total active             
0.2%  AN   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
__________________________________________________________________________
Unpreserved control                                                       
Organism                                                                  
      48 Hours                                                            
             7 Days 14 Days                                               
                          21 Days                                         
                                 28 Days                                  
__________________________________________________________________________
AN    4,000,000                                                           
CAN   160,000                                                             
             &gt;100,000,000                                                 
                    5,600,000                                             
                          &gt;100,000,000                                    
                                 &gt;1,000,000                               
ECOLI        &gt;100,000,000                                                 
                    800,000,000                                           
                          48,000,000                                      
                                 600,000                                  
PC    &gt;100,000,000                                                        
             50,000,000                                                   
                    48,000,000   &gt;1,000,000                               
PSA   &gt;100,000,000                                                        
             &gt;100,000,000                                                 
                    58,000,000                                            
                          65,000,000                                      
                                 &gt;1,000,000                               
SA           &gt;100,000,000                                                 
__________________________________________________________________________
Inoculum Concentration                                                    
Organism     0 Hours                                                      
                  21 Days                                                 
__________________________________________________________________________
AN           380,000                                                      
                  330,000                                                 
CAN          860,000                                                      
                  4,200,000                                               
ECOLI        2,500,000                                                    
                  5,300,000                                               
PC           1,900,000                                                    
                  60,000,000                                              
PSA          3,200,000                                                    
                  5,000,000                                               
SA           2,400,000                                                    
                  3,000,000                                               
__________________________________________________________________________
 
    
     Table 25 shows a similar study as in Table 24 above in which the vehicle for the composition was the typical emulsion described above. 
     
                                           TABLE 25                                
__________________________________________________________________________
Test Level                                                                
      Organism                                                            
           48 Hours                                                       
                7 Days                                                    
                      14 Days                                             
                           21 Days                                        
                                  28 Days                                 
__________________________________________________________________________
99.5% GII/0.5% IPBC/propylene glycol solution - 0.05% total active        
 0.05%                                                                    
      AN   40   &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  520,000                                                        
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           320  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   24,000                                                         
                &lt;10   &lt;10  &lt;10    &lt;10                                     
99.5% GII/0.5% IPBC/propylene glycol solution - 0.1% total active         
0.1%  AN   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           10   &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   650  &lt;10   &lt;10  &lt;10    &lt;10                                     
99.5% GII/0.5% IPBC/propylene glycol solution - 0.2% total active         
0.2%  AN   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
99% GII/1% IPBC/propylene glycol solution - 0.05% total active            
 0.05%                                                                    
      AN   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  6,700                                                          
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           66,000                                                         
                &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   50,000                                                         
                &lt;10   &lt;10  &lt;10    &lt;10                                     
99% GII/1% IPBC/propylene glycol solution - 0.1% total active             
0.1%  AN   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   620  &lt;10   &lt;10  &lt;10    &lt;10                                     
99% GII/1% IPBC/propylene glycol solution - 0.2% total active             
0.2%  AN   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     CAN  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     ECOLI                                                               
           &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PC   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     PSA  &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
&#34;     SA   &lt;10  &lt;10   &lt;10  &lt;10    &lt;10                                     
__________________________________________________________________________
Unpreserved control                                                       
Organism                                                                  
      48 Hours                                                            
             7 Days 14 Days                                               
                          21 Days                                         
                                 28 Days                                  
__________________________________________________________________________
AN    3,100,000                                                           
             650,000                                                      
                    370,000                                               
                          1,400,000                                       
                                 260,000                                  
CAN   6,000,000                                                           
             4,000,000                                                    
                    1,100,000                                             
                          5,800,000                                       
                                 &gt;1,000,000                               
ECOLI 11,000,000                                                          
             7,300,000                                                    
                    6,000,000                                             
                          730,000                                         
                                 220,000                                  
PC    100,000,000                                                         
             53,000,000                                                   
                    40,000,000                                            
                          40,000,000                                      
                                 600,000                                  
PSA   5,000,000                                                           
             500,000                                                      
                    2,700,000                                             
                          72,000 20,000                                   
SA    30,000,000                                                          
             150,000                                                      
                    440,000                                               
                          2,500  9,900                                    
__________________________________________________________________________
Inoculum Concentration                                                    
Organism     0 Hours                                                      
                  21 Days                                                 
__________________________________________________________________________
AN           380,000                                                      
                  330,000                                                 
CAN          860,000                                                      
                  4,200,000                                               
ECOLI        2,500,000                                                    
                  5,300,000                                               
PC           1,900,000                                                    
                  60,000,000                                              
PSA          3,200,000                                                    
                  5,000,000                                               
SA           2,400,000                                                    
                  3,000,000                                               
__________________________________________________________________________
 
    
     The results shown in Tables 24 and 25 demonstrate that the compositions of the invention are completely effective against the tested organisms in comparison to the unpreserved controls. 
     While the invention has been described with particular reference to certain embodiments thereof, it will be understood that changes and modifications may be made which are within the skill of the art. Accordingly, it is intended to be bound only by the following claims, in which: