Patent Publication Number: US-2021170394-A1

Title: Liquid feeding device

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     The present application claims priority under 35 U.S.C. § 119 to Japanese Patent Application No. 2019-222330 filed on Dec. 9, 2019. Each of the above application is hereby expressly incorporated by reference, in its entirety, into the present application. 
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The technology of the present disclosure relates to a liquid feeding device. 
     2. Description of the Related Art 
     Test containers such as a test cartridge, an analysis chip, and the like used for performing various analysis with respect to a specimen extracted from a biological sample are known. 
     JP2007-101428A discloses a cartridge for a chemical treatment comprising a plurality of wells (liquid accommodation portions) accommodating a liquid and configured by stacking an elastic member comprising a plurality of recesses on one surface on a substrate so that the recesses face the substrate side, and a flow path connecting between the wells. JP2007-101428A discloses a method for rotating a roller while pressing the elastic member of a cartridge for elastic deformation of the elastic member, to cause pressing of a liquid in the elastically deformed well to move to an adjacent well via the flow path connected to the well. 
     JP2003-166910A discloses a liquid feeding mechanism which feeds a liquid filled in a liquid tank to a flow path connected to the liquid tank by changing a volume of the liquid tank (liquid accommodation portion) formed to surround a wall, and an analysis device comprising the liquid feeding mechanism. 
     SUMMARY OF THE INVENTION 
     For example, in order to perform nucleic acid extraction and analysis, it is necessary to provide a test container comprising at least three accommodation portions from an upstream side to a downstream side in a liquid feeding direction, in which the liquid is fed so that a liquid return to the upstream accommodation portion does not occur, in a case where the liquid is fed from the accommodation portion in the middle of the three accommodation portions to the downstream accommodation portion side. 
     In JP2007-101428A, the liquid is fed to the downstream side in a state where the upstream side is crushed with respect to the accommodation portion containing the liquid and the flow path is blocked, and accordingly, the liquid return to the upstream side does not occur. However, in JP2007-101428A, it is premised that all the liquid accommodated in the upstream accommodation portion is moved to the downstream side. Accordingly, the aspect of proceeding to the next step while remaining the liquid in the upstream accommodation portion cannot be applied. Meanwhile, JP2003-166910A discloses only liquid feeding between two accommodation portions, and a method for preferentially feeding a liquid to the downstream side from an accommodation portion, of which flow paths are connected to both the upstream side and the downstream side is not considered. 
     In addition, in the test container disclosed in both JP2007-101428A and JP2003-166910A, the flow path connecting the liquid accommodation portions is disposed to connect lower ends of the liquid accommodation portions, and accordingly, even in a case where an external force is not applied, the liquid may pass the flow path and flow into the adjacent accommodation portion due to a capillary force or the like. 
     The technology of the disclosure is made in view of the above circumstance, and an object thereof is to provide a liquid feeding device including a test container, comprising at least three accommodation portions accommodating a liquid, and capable of preventing a liquid return to an upstream accommodation portion, in a case where the liquid accommodated in a middle accommodation portion of the three accommodation portions is fed to a downstream accommodation portion. 
     According to a first aspect of the disclosure, there is provided a liquid feeding device, comprising: a test container including a first accommodation portion, a second accommodation portion, and a third accommodation portion each accommodating a liquid, a first flow path connecting the first accommodation portion and the second accommodation portion to each other at respective upper end positions thereof, and a second flow path connecting the second accommodation portion and the third accommodation portion to each other at respective upper end positions thereof, in which at least a portion forming an upper wall surface of the second accommodation portion has flexibility; and 
     a pressing machine which includes a pressing portion and presses the portion forming the upper wall surface of the second accommodation portion at a position closer to the first flow path from a center in a liquid feeding direction towards an inside of the second accommodation portion from an outside by the pressing portion, 
     in which the liquid accommodated in the second accommodation portion is fed to the third accommodation portion by pressing the portion forming the upper wall surface of the second accommodation portion with the pressing machine. 
     According to a second aspect of the disclosure, there is provided a liquid feeding device, comprising: a test container including a first accommodation portion, a second accommodation portion, and a third accommodation portion each accommodating a liquid, a first flow path connecting the first accommodation portion and the second accommodation portion to each other at respective upper end positions thereof, and a second flow path connecting the second accommodation portion and the third accommodation portion to each other at respective upper end positions thereof, in which at least a first portion forming an upper wall surface of the first flow path and a second portion forming an upper wall surface of the second accommodation portion has flexibility; and 
     a pressing machine which includes a first pressing portion and a second pressing portion, presses the first portion forming the upper wall surface of the first flow path towards the inside of the first flow path from outside by the first pressing portion, and presses the second portion forming the upper wall surface of the second accommodation portion towards an inside of the second accommodation portion from an outside by the second pressing portion, 
     in which the liquid accommodated in the second accommodation portion is fed to the third accommodation portion by pressing the first portion and the second portion with the pressing machine. 
     In the liquid feeding device of the second aspect of the present disclosure, the pressing machine may simultaneously perform a pressing operation by the first pressing portion and a pressing operation by the second pressing portion. 
     In the liquid feeding device of the second aspect of the present disclosure, the pressing machine may perform a pressing operation by the first pressing portion before a pressing operation by the second pressing portion, and may perform the pressing operation by the second pressing portion while maintaining a pressing state by the first pressing portion. 
     In the liquid feeding device according to the first and second aspects of the present disclosure, the test container may include a main body portion in which a portion forming each of the first accommodation portion, the first flow path, the second accommodation portion, the second flow path, and the third accommodation portion is open, and an upper lid member including the portion forming the upper wall surface of the second accommodation portion, and the first accommodation portion, the first flow path, the second accommodation portion, the second flow path, and the third accommodation portion may be internally formed by covering the opening of the main body portion with the upper lid member. 
     In the liquid feeding device of the first and second aspects of the present disclosure, the upper lid member of the test container may have flexibility over an entire region. 
     In the liquid feeding device according to the first and second aspects of the present disclosure, the test container preferably comprises a liquid return prevention structure which prevents a backflow of the liquid to the first accommodation portion, in a case where the liquid accommodated in the second accommodation portion is fed to the third accommodation portion via the second flow path. 
     In the liquid feeding device according to the first and second aspects of the present disclosure, the liquid return prevention structure of the test container may have a structure in which a height from an inner bottom surface of the second accommodation portion to an inner bottom surface of the first flow path is higher than a height from the inner bottom surface of the second accommodation portion to an inner bottom surface of the second flow path. 
     In the liquid feeding device according to the first and second aspects of the disclosure, the liquid return prevention structure may have a structure of the first flow path and the second flow path in which a water contact angle of an inner surface of the first flow path is set to be greater than a water contact angle of an inner surface of the second flow path. 
     In the liquid feeding device according to the first and second aspects of the disclosure, the liquid return prevention structure may have a structure of a stepped portion which is provided between the first flow path and the second accommodation portion and which includes two or more steps from an inner bottom surface of the first accommodation portion. 
     In the liquid feeding device according to the first and second aspects of the present disclosure, the test container may further comprise a chromatographic carrier for performing a nucleic acid test; and a carrier accommodation portion accommodating the chromatographic carrier. 
     In the liquid feeding device according to the first and second aspects of the present disclosure, in the test container, the first accommodation portion may accommodate a first liquid containing magnetic particles, the first flow path allows separated magnetic particles separated from the first liquid to pass through the first flow path, and the second accommodation portion may accommodate the separated magnetic particles. 
     The liquid feeding device according to the first and second aspects of the present disclosure may further comprise a magnetic field generation and movement unit including a magnet and a movement mechanism which moves the magnet, the magnet may be disposed on the first accommodation portion of the test container to collect the magnet particles in the first liquid and the magnet is moved onto the second accommodation portion from the first accommodation portion along the first flow path, so that the separated magnetic particles separated from the first liquid passes through the first flow path to be moved to the second accommodation portion. 
     According to the liquid feeding device of the disclosure, in a liquid feeding device including a test container, comprising at least three accommodation portions accommodating a liquid, and capable of preventing a liquid return to an upstream accommodation portion, in a case where the liquid accommodated in a middle accommodation portion of the three accommodation portions is fed to a downstream accommodation portion. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a diagram showing a schematic configuration of a liquid feeding device  201 . 
         FIG. 2  is an exploded perspective view showing a schematic configuration of a test container  211 . 
         FIG. 3  is an cross-sectional view showing a schematic configuration of the test container  211 . 
         FIG. 4  is a plan view showing a schematic configuration of a main body portion of the test container  211 . 
         FIG. 5  is a diagram showing a liquid feeding method of the liquid feeding device  201 . 
         FIG. 6  is a diagram showing a schematic configuration of a liquid feeding device  202 . 
         FIG. 7  is a diagram showing a liquid feeding method of the liquid feeding device  202 . 
         FIG. 8  is an exploded perspective view showing a schematic configuration of a test container  1 . 
         FIG. 9  is an cross-sectional view showing a schematic configuration of the test container  1 . 
         FIG. 10  is a plan view showing a schematic configuration of a main body portion of the test container  1 . 
         FIG. 11  is an cross-sectional view showing a schematic configuration of a test container  1 A of a modification example. 
         FIG. 12  is an exploded perspective view showing a schematic configuration of a test container  2 . 
         FIG. 13  is an cross-sectional view showing a schematic configuration of the test container  2 . 
         FIG. 14  is a plan view showing a schematic configuration of a main body portion of the test container  2 . 
         FIG. 15  is an exploded perspective view showing a schematic configuration of a test container  3 . 
         FIG. 16  is an cross-sectional view showing a schematic configuration of the test container  3 . 
         FIG. 17  is a plan view showing a schematic configuration of a main body portion of the test container  3 . 
         FIG. 18  is an cross-sectional view showing a schematic configuration of a test container  3 A of a modification example. 
         FIG. 19  is an cross-sectional view showing a schematic configuration of a test container  4 . 
         FIG. 20  is an cross-sectional view showing a schematic configuration of a test container  5 . 
         FIG. 21  is an cross-sectional view showing a schematic configuration of a test container  6 . 
         FIG. 22  is a schematic configuration diagram of a nucleic acid extraction test device  100 . 
         FIG. 23  is an exploded perspective view of a test container and a diagram showing a main part of a dispenser. 
         FIG. 24  is a diagram showing a cross-sectional view of a test container and a magnet. 
         FIG. 25  is a diagram showing a cross-sectional view of a test container and a main part of a pressing machine. 
         FIG. 26  is a diagram for explaining pressed portions in the liquid feeding methods of examples and comparative examples. 
     
    
    
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     Hereinafter, an example of an embodiment according to the present invention will be described with reference to the drawings. A front direction, a rear direction, an upward direction, a downward direction, a left direction, and a right direction used in the description below correspond to “FR”, “RR”, “UP”, “DO”, “LH”, and “RH”, respectively, in the each drawing. Since these directions are defined for convenience of description, a device configuration is not limited to these directions. The FR side is an upstream side and the RR side is a downstream side in the use of a container. In addition, the scales and the like of the respective constituent elements in the drawings are suitably changed from the actual scales for the sake of easy visual recognition. 
     Liquid Feeding Device of First Embodiment 
     A liquid feeding device of the first embodiment will be described.  FIG. 1  is a perspective view showing a schematic configuration of a liquid feeding device  201 . The liquid feeding device  201  comprises a test container  211  and a pressing machine  50  having a plunger  52  as a pressing portion. 
     Test Container 
       FIG. 2  is an exploded perspective view of the test container  211 .  FIG. 2  is an cross-sectional view showing a schematic configuration of the test container  211 .  FIG. 3  is a plan view showing a schematic configuration of a main body portion  212  of the test container  211 . 
     The test container  211  comprises the container main body  210  including a first accommodation portion  221 , a second accommodation portion  222 , and a third accommodation portion  223  each accommodating a liquid, a first flow path  231  connecting the first accommodation portion  221  and the second accommodation portion  222  to each other at respective upper end positions thereof, and a second flow path  232  connecting the second accommodation portion  222  and the third accommodation portion  223  to each other at respective upper end positions thereof. The container main body  210  has flexibility so that it can be deformed toward the inside of the second accommodation portion  222  at least in the portion  214 A forming the upper wall surface  222   b  of the second accommodation portion  222 . 
     In this example, the container main body  210  comprises a main body portion  212  and an upper lid member  214 . The main body portion  212  has an opening in a portion forming each of the first accommodation portion  221 , the first flow path  231 , the second accommodation portion  222 , the second flow path  232 , and the third accommodation portion  223 . The container main body  210  has a configuration in which the first accommodation portion  221 , the first flow path  231 , the second accommodation portion  222 , the second flow path  232 , and the third accommodation portion  223  are formed therein by covering the opening of the main body portion  212  with the upper lid member  214 . In other words, the container main body  210  configures the inner bottom surfaces  221   a  to  223   a  and the side wall surfaces of the accommodation portions  221  to  223 , and the inner bottom surfaces  231   a  and  232   a  and the side wall surfaces of the flow paths  231  and  232 , and the upper lid member  214  configures the upper wall surfaces  221   b  to  223   b  of the accommodation portions  221  to  223  and the upper wall surfaces  231   b  and  232   b  of the flow paths  231  and  232 . However, the present invention is not limited to this configuration, as long as it has a configuration of comprising each accommodation portion and each flow path therein. 
     In this example, the upper lid member  214  has flexibility throughout. However, the entire upper lid member  214  does not have to be flexible, as long as the portion  214 A configuring at least the upper wall surface  222   b  of the second accommodation portion  222  of the container main body  210 , that is, the portion  214 A of the upper lid member  214  has a flexible portion deformable in a direction toward the second accommodation portion  222 . 
     The test container  211  comprises the first flow path  231  at the upper end position of the first accommodation portion  221  and the second accommodation portion  222 , and the second flow path  232  at the upper end position of the second accommodation portion  222  and the third accommodation portion  223 , respectively. Accordingly, the liquid accommodated in the accommodation portion is difficult to flow into the flow path, compared to a case where the flow path is comprised at a lower end or in the middle in a depth direction. Therefore, it is possible to prevent a passage of the liquid into the flow path due to a capillary phenomenon or the like without applying an external force. Meanwhile, since the portion  214 A deformable toward the inside of the second accommodation portion  222  is comprised at the upper portion of the second accommodation portion  222 , the portion  214 A is deformed toward the inside of the second accommodation portion  222  to reduce a volume of the second accommodation portion  222 , thereby easily realizing liquid feeding to the third accommodation portion  223  by pushing the liquid accommodated in the second accommodation portion  222 . 
     Pressing Machine 
     The pressing machine  50  presses the portion forming the upper wall surface  222   b  of the second accommodation portion  222  of the test container  211 , that is, the portion  214 A of the container towards the inside of the second accommodation portion  222  by the plunger  52 . In this example, the pressing machine  50  further comprises a cylinder  54  which guides the plunger  52  during the pressing operation. 
     The pressing machine  50  is configured to press a position of the portion  214 A of the test container  211  closer to the first flow path  231  from the center in the liquid feeding direction by the plunger  52 . By setting the test container  211  on the liquid feeding device  201 , the liquid feeding device  201  may be configured so the plunger  52  and the portion  214 A of the test container  211  are positioned to have the above relationship, and the plunger  52  and the test container  211  can relatively move so that the plunger  52  of the pressing machine  50  is at a position closer to the first flow path  231  of the portion  214 A. For example, the liquid feeding device  1  may include a movement mechanism for the plunger  52  and a movement mechanism of the test container  211 . 
       FIG. 5  shows a state before the pressing by the pressing machine  50  (upper diagram) and a state at the time of pressing (lower diagram). As shown in the lower diagram of  FIG. 5 , the pressing machine  50  presses the portion  214 A toward the inside of the second accommodation portion  222  with the plunger  52 , thereby deforming the portion  214 A forming the upper wall surface  222   b  of the second accommodation portion  222  to the second accommodation portion  222  side. Accordingly, the volume of the second accommodation portion  222  is reduced and a liquid L in the second accommodation portion  222  is fed to the third accommodation portion  223 . 
     In the liquid feeding device  201 , the pressing machine  50  presses the position of the portion  214 A of the test container  211  closer to the first flow path  231  with the plunger  52 , so that the liquid accommodated in the second accommodation portion  222  moves more to the second flow path  232  side. Accordingly, it is possible to feed a larger amount of liquid to the third accommodation portion  223  and to relatively suppress the amount of a backflow to the first accommodation portion  221  side. 
     Here, in the plan view shown in  FIG. 4 , “the position of the portion forming the upper wall surface of the second accommodation portion closer to the first flow path” refers to the position of the portion  214 A corresponding to the upper wall surface  222   b  of the second accommodation portion  222  in a region (shaded region in  FIG. 4 ) closer to the first flow path  231  than a line B 1  orthogonal to a center O 1  of a licloser to line A 1  connecting a boundary C 1  with the first flow path  231  of the second accommodation portion  222  and the boundary C 2  with the second flow path  232  of the second accommodation portion  222 . Here, in a case where the boundary C 1  between the first flow path  231  and the second accommodation portion  222  is at 0% position and the boundary C 2  between the second accommodation portion  222  and the second flow path  231  is at 100% position, the center O 1  is 50% position, and the position closer to the first flow path  231  is a position less than the 50% position. The pressed position is preferably 10% to 30%, and more preferably 10% to 15%. 
     The pressing portion included in the pressing machine  50  is not limited to the plunger as long as it can press the portion  214 A toward the inside of the second accommodation portion  222 , and a rod-shaped pressing indenter, a cylinder and the like can be selected. In addition, as for a tip shape, it is possible to appropriately select a shape such as a cylinder, a prism, a hemisphere, a cone, a polygonal pyramid, a flat shape, or a wedge shape. 
     The portion  214 A of the container main body  210  that forms the upper wall surface  222   b  of the second accommodation portion  222  may be pushed toward the inside of the second accommodation portion  222  to reduce the volume of the second accommodation portion  222  and is not limited to the portion  214 A having flexibility throughout. For example, the portion of the portion  214 A which directly comes into contact with the plunger  52  may not have flexibility and only the surrounding portion thereof may have flexibility. 
     In a case where the portion  214 A is formed of a flexible film or in a case where the entire upper lid member  214  is formed of a flexible film, a breaking elongation of the flexible film is preferably 100% to 600%, more preferably 200% to 500%, and even more preferably 200% to 400%. In addition, in a case where a thickness of the flexible film is t μm, a modulus of elasticity of the flexible film is α MPa (megapascal), and a depth of the second accommodation portion  22  is d μm, relationships of 0.03≤t/d≤2.5 and 2,000≤α×t≤250,000 are preferably satisfied. The relationships of 0.03≤t/d≤1.8 and 2,000≤α×t≤110,000 are more preferably satisfied, relationships of 0.08≤t/d≤1.0 and 2,000≤α×t≤50,000 are even more preferably satisfied, and relationships of 0.2≤t/d≤0.4 and 4,000≤α×t≤20,000 are particularly preferably satisfied. 
     As a material of the flexible film, a silicone resin, a fluororesin, polyolefin, polycarbonate, and the like are suitable. 
     A dispensing port for dispensing a liquid may be provided in a portion of the upper lid member  214  that forms each of the upper wall surfaces  221   b ,  222   b , and  223   b  of the first accommodation portion  221 , the second accommodation portion  222 , and the third accommodation portion  223 . The dispensing port is opened at the time of dispensing but is preferably sealed at other times. Alternatively, the upper lid member  214  may be provided with no dispensing port, and the upper lid member  214  may be covered and adhered to an upper surface of the main body portion  212  after injecting the liquid to each of the accommodation portions  221 ,  222 , and  223 . 
     In this example, the first flow path  231  and the second flow path  232  have a width narrower than a width of the first accommodation portion  221  and the second accommodation portion  222 . The widths of the first flow path  231  and the second flow path  232  may be the same as the widths of the first accommodation portion  221  and the second accommodation portion  222 , but smaller width than the widths of the first accommodation portion  221  and the second accommodation portion  222  is more preferable. The width of the flow path  230  is preferably ½ or less and more preferably ⅓ or less of the width of the first accommodation portion  221 . The width of the first flow path  231  and the width of the second flow path  232  may or may not be the same. 
     As the material of the container main body  210 , that is, the main body portion  212 , any known resin-molded plastic materials can be used without particular limitation. Examples thereof include an acrylic resin such as a polymethyl methacrylate resin (PMMA), a polyolefin resin such as a polycarbonate resin, polyethylene (PE), polypropylene (PP), an ethylene-vinyl acetate copolymer (EVA), a cycloolefin resin such as a cycloolefin polymer (COP) and a cyclic olefin copolymer (COC), a silicone resin, a fluororesin, a polystyrene resin, a polyvinyl chloride resin, a phenol resin, a urethane resin, a polyester resin, an epoxy resin, and a cellulose resin. Particularly, from viewpoints of heat resistance and transparency, a polycarbonate resin, polypropylene, a cycloolefin resin, a silicone resin, and a fluororesin are preferable. In addition, a copolymer of these resins may be used. 
     A size (volume) of the first accommodation portion  221 , the second accommodation portion  222 , and the third accommodation portion  223  is, for example, approximately 1 μL (microliter) to several hundreds μL. 
     Liquid Feeding Device of Second Embodiment 
     A liquid feeding device of the second embodiment will be described.  FIG. 6  is a perspective view showing a schematic configuration of a liquid feeding device  202 . The liquid feeding device  202  comprises a test container  211  and a pressing machine  55  having a plunger  51  as the first pressing portion and a plunger  52  as the second pressing portion. 
     The test container  211  is the same as that of the first embodiment, and is formed of the main body portion  212  and the upper lid member  214  having flexibility over the entire body. However, the test container used in the liquid feeding device  201  of the second embodiment may comprise first accommodation portion  221 , a second accommodation portion  222 , and a third accommodation portion  223  each accommodating a liquid, a first flow path  231  connecting the first accommodation portion  221  and the second accommodation portion  222  to each other at respective upper end positions thereof, and a second flow path  232  connecting the second accommodation portion  222  and the third accommodation portion  223  to each other at respective upper end positions thereof, in which at least the first portion  214 B (see  FIG. 2 ) forming an upper wall surface  231   b  of the first flow path  231  and a second portion  214 A forming an upper wall surface  222   b  of the second accommodation portion  222  has flexibility. 
     Pressing Machine 
     The pressing machine  55  presses the first portion  214 B forming the upper wall surface  231   b  of the first flow path  231  of the test container  211  towards the inside of the first flow path  231  by the first plunger  51 , and presses the second portion  214 A forming the upper wall surface  222   b  of the second accommodation portion  222  of the test container  211  towards the inside of the second accommodation portion  222  by the second plunger  52 . The pressing machine  50  further comprises a cylinder  53  which guides the plunger  51 , and a cylinder  54  which guides the movement of the plunger  52  during the pressing operation. The pressing machine  55  comprises the plungers  51  and  52  so as to press the first portion  214 B and the second portion  214 A of the test container  211 , respectively. By setting the test container  211  on the liquid feeding device  202 , the liquid feeding device  202  may be configured so the plunger  51  and the plunger  52  and the first portion  214 B and the second portion  214 A of the test container  211  are positioned to have the above relationship, and the plunger  51  is provided to relatively move with respect to the test container  211 , so that the plunger  51  of the pressing machine  50  is positioned on the first portion  214 B, and the plunger  52  is provided to relatively move with respect to the test container  211 , so that the plunger  52  is positioned on the first portion  214 A. For example, the liquid feeding device  201  may comprise a movement mechanism that moves the plungers  51  and  52  independently or together, a movement mechanism for the test container, or both. 
       FIG. 7  shows a state before the pressing by the pressing machine  55  (upper diagram) and a state at the time of pressing (lower diagram). As shown in the lower diagram of  FIG. 7 , the pressing machine  55  presses the portion  214 A toward the inside of the second accommodation portion  222  with the plunger  52 , thereby deforming the second portion  214 A forming the upper wall surface  222   b  of the second accommodation portion  222  to the second accommodation portion  222  side. Accordingly, the volume of the second accommodation portion  222  is reduced and a liquid L in the second accommodation portion  222  is fed to the third accommodation portion  223 . In this case, the pressing machine  55  presses the first portion  214 B towards the inside of the first flow path  231  with the plunger  51 , the first portion  214 B forming the upper wall surface  222   b  of the second accommodation portion  222  is deformed to the first flow path  231  side, and accordingly, the first flow path  231  is blocked so that the liquid L pushed from the second accommodation portion  222  does not pass through the first flow path  231 . 
     In the liquid feeding device  201 , the pressing machine  55  presses the first portion  214 B with the first plunger  51  to block the first flow path  231  and presses the second portion  214 A with the second plunger  52 . Therefore, the liquid accommodated in the second accommodation portion  222  hardly passes through the first flow path  231  and it is possible to effectively prevent the backflow to the first accommodation portion  221 , thereby allowing a larger amount of liquid L to flow into the third accommodation portion  223 . 
     The pressing machine  55  may perform the pressing operation by the first plunger  51  and the pressing operation by the second plunger  52  at the same time, or the pressing operation by the first plunger  51  may be performed before the pressing operation by the second plunger  52 . The pressing operation by the second plunger  52  may be performed while the pressing state by the first plunger  51  is maintained. 
     The pressing machine  55  first presses the first flow path  231  with the first plunger  51 , and performs a pressing operation with the second plunger  52  while maintaining the state, so that the amount of liquid passing through the first flow path  231  can be further reduced. 
     In a case of pressing the first portion  214 B with the first plunger  51 , in the flow path length direction of the first flow path  231 , in a case where the boundary C 0  with the first accommodation portion  221  is set as the 0% position and the boundary C 1  with the second accommodation portion  222  is set as the 100% position, it is preferable to press the second accommodation portion  222  side from the 25% position, more preferable to press the second accommodation portion  222  side from the 50% position, and particularly preferable to press the position of 50% to 75%. 
     The first pressing portion provided in the pressing machine  55  may be configured to press the first portion  214 B toward the inside of the first flow path  231 , and is not limited to the plunger. In the same manner, the second pressing portion may be configured to press the second portion  214 A toward the inside of the second accommodation portion  222 , and is not limited to the plunger. 
     In the liquid feeding device  201  of the first embodiment and the liquid feeding device  202  of the second embodiment, instead of the test container  211 , it is more preferable to comprise the test container comprising the liquid return prevention structure which prevents a backflow of the liquid L to the first accommodation portion  221 , in a case where the liquid L accommodated in the second accommodation portion  222  is fed to the third accommodation portion  223  via the second flow path  232 . An example of a test container comprising the liquid return prevention structure will be described below. 
     Test Container  1   
     The test container  1  will be described.  FIG. 8  is an exploded perspective view showing a schematic configuration of a test container  1 .  FIG. 9  is an cross-sectional view showing a schematic configuration of the test container  1 .  FIG. 10  is a plan view showing a schematic configuration of a main body portion  12  of the test container  1 . 
     The test container  1  shown in  FIGS. 8, 9, and 10  comprises the container main body  10  including a first accommodation portion  21 , a second accommodation portion  22 , and a third accommodation portion  23  each accommodating a liquid, a first flow path  31  connecting the first accommodation portion  21  and the second accommodation portion  22  to each other at respective upper end positions thereof, and a second flow path  32  connecting the second accommodation portion  22  and the third accommodation portion  23  to each other at respective upper end positions thereof. The container main body  10  has at least a portion  14 A forming an upper wall surface  22   b  of the second accommodation portion  22  having flexibility to be deformable inwards of the second accommodation portion  22 . In a case of applying to the liquid feeding device  202 , the portion  14 B forming the upper wall surface  31   b  of the first flow path  31  further has flexibility deformable toward the inside of the first flow path  31 . 
     In this example, the container main body  10  comprises a main body portion  12  and an upper lid member  14 . The main body portion  12  has an opening in a portion forming each of the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23 . The container main body  10  has a configuration in which the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23  are formed therein by covering the opening of the main body portion  12  with the upper lid member  14 . In other words, the main body portion  12  configures the inner bottom surfaces  21   a  to  23   a  and the side wall surfaces of the accommodation portions  21  to  23 , and the inner bottom surfaces  31   a  and  32   a  and the side wall surfaces of the flow paths  31  and  32 , and the upper lid member  14  configures the upper wall surfaces  21   b  to  23   b  of the accommodation portions  21  to  23  and the upper wall surfaces  31   b  and  32   b  of the flow paths  31  and  32 . However, the present invention is not limited to this configuration, as long as it has a configuration of comprising each accommodation portion and each flow path therein. 
     In this example, the upper lid member  14  has flexibility throughout. However, the entire upper lid member  14  does not have to be flexible, as long as the portion  14 A configuring at least the upper wall surface  22   b  of the second accommodation portion  22  of the container main body  10 , that is, the portion  14 A of the upper lid member  14  has a flexible portion deformable in a direction toward the second accommodation portion  22 . 
     As a liquid return prevention structure, the test container  1  has a structure in which a height h 1  from the inner bottom surface  22   a  of the second accommodation portion  22  to the inner bottom surface  31   a  of the first flow path  31  (hereinafter, referred to as a “height h 1  of the first flow path”) is higher than a height h 2  from the inner bottom surface  22   a  of the second accommodation portion  22  to the inner bottom surface  32   a  of the second flow path  32  (hereinafter, referred to as a “height h 2  of the second flow path”). In the test container  1 , the height h 1  of the inner bottom surface  31   a  of the first flow path  31  from the inner bottom surface  22   a  of the second accommodation portion  22  is defined as a height of a corner of a level difference portion between the first flow path  31  and the second accommodation portion  22  from the inner bottom surface  22   a  of the second accommodation portion  22 . In the same manner, the height h 2  of the inner bottom surface  32   a  of the second flow path  32  from the inner bottom surface  22   a  of the second accommodation portion  22  is defined as a height of a corner of a level difference portion between the second accommodation portion  22  and the second flow path  32  from the inner bottom surface  22   a  of the second accommodation portion  22 . The liquid return prevention structure is a structure for preventing a backflow of the liquid to the first accommodation portion  21 , in a case where the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the second flow path  32  due to the deformation of the portion  14 A forming the upper wall surface  22   b  of the second accommodation portion  22  in a direction toward the second accommodation portion  22 . 
     The test container  1  comprises the first flow path  31  at the upper end position of the first accommodation portion  21  and the second accommodation portion  22 , and the second flow path  32  at the upper end position of the second accommodation portion  22  and the third accommodation portion  23 , respectively. Accordingly, the liquid accommodated in the accommodation portion is difficult to flow into the flow path, compared to a case where the flow path is comprised at a lower end or in the middle in a depth direction. Therefore, it is possible to prevent a passage of the liquid into the flow path due to a capillary phenomenon or the like without applying an external force. Meanwhile, since the portion  14 A deformable toward the inside of the second accommodation portion  22  is comprised at the upper portion of the second accommodation portion  22 , the portion  14 A is deformed toward the inside of the second accommodation portion  22  to reduce a volume of the second accommodation portion  22 , thereby easily realizing liquid feeding to the third accommodation portion  23  by pushing the liquid accommodated in the second accommodation portion  22 . 
     Since the height h 1  of the first flow path  31  is higher than the height h 2  of the second flow path  32 , in a case where the portion  14 A of the container main body  10  is deformed in the direction toward the second accommodation portion  22  so that the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the second flow path  32 , the liquid pushed from the second accommodation portion  22  is preferentially fed to the second flow path  32  formed at a lower position. Accordingly, the liquid return to the first flow path  31  can be suppressed, and the liquid feeding properties to the third accommodation portion  23  at a downstream side is high. According to this configuration, it is possible to suppress the liquid return to the first flow path  31  and increase the liquid feeding properties to the third accommodation portion  23  with a simple configuration of providing a difference between the heights h 1  and h 2 . 
     A difference h 1 −h 2  between the height h 1  of the first flow path  31  and the height h 2  of the second flow path  32  is preferably 20% or more, more preferably 30% or more, and even more preferably 50% or more of the height h 2  of the second flow path  32 . As the difference h 1 −h 2  is large, the liquid feeding to the second flow path  32  is further promoted, and the liquid feeding properties to the third accommodation portion  23  can be increased. 
     Test Container  1 A 
       FIG. 11  shows a test container  1 A which is a modification example of the test container  1 . The test container  1 A comprises a container main body  10 A consisting of a main body portion  12 A and an upper lid member  14 . In the test container  1 A, a corner  33  formed by an inner bottom surface  31   a  of the first flow path  31  and an inner side surface  22   c  of the second accommodation portion  22  in a level difference portion between the inner bottom surface  31   a  of the first flow path  31  and the second accommodation portion  22  has an acute angle. By setting the corner  33  of the level difference portion to have an acute angle, it is possible to more effectively suppress the flow of the liquid accommodated in the second accommodation portion  22  to the first flow path, compared to a case where the angle is equal to or greater than 90°. Therefore, it is possible to more preferentially feed the liquid accommodated in the second accommodation portion  22  to the second flow path  32 . 
     Test Container  2   
     The test container  2  will be described.  FIG. 12  is an exploded perspective view showing a schematic configuration of a test container  2 .  FIG. 13  is an cross-sectional view showing a schematic configuration of the test container  2 .  FIG. 14  is a plan view showing a schematic configuration of a main body portion  12 B of the test container  2 . 
     The test container  2  shown in  FIGS. 12, 13, and 14  comprises the container main body  10 B including a first accommodation portion  21 , a second accommodation portion  22 , and a third accommodation portion  23  each accommodating a liquid, a first flow path  31  connecting the first accommodation portion  21  and the second accommodation portion  22  to each other at respective upper end positions thereof, and a second flow path  32  connecting the second accommodation portion  22  and the third accommodation portion  23  to each other at respective upper end positions thereof. The container main body  10 B has at least the portion  14 A forming the upper wall surface  22   b  of the second accommodation portion  22  having flexibility to be deformable inwards of the second accommodation portion  22 . In a case of applying to the liquid feeding device  202 , the portion  14 B forming the upper wall surface  31   b  of the first flow path  31  further has flexibility deformable toward the inside of the first flow path  31 . In the drawings, the same reference numerals are used for the same elements as those of the test container  1 . Elements having the same reference numerals as those of the test container  1  are the same as those described for the test container  1 , and specific description thereof will be omitted. The same applies to the following drawings. 
     In this example, the container main body  10 B comprises the main body portion  12 B and the upper lid member  14 . The main body portion  12 B has an opening in a portion forming each of the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23 . The container main body  10 B has a configuration in which the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23  are formed therein by covering the opening of the main body portion  12 B with the upper lid member  14 . In other words, the main body portion  12 B configures the inner bottom surfaces  21   a  to  23   a  and the side wall surfaces of the accommodation portions  21  to  23 , and the inner bottom surfaces  31   a  and  32   a  and the side wall surfaces of the flow paths  31  and  32 , and the upper lid member  14  configures the upper wall surfaces  21   b  to  23   b  of the accommodation portions  21  to  23  and the upper wall surfaces  31   b  and  32   b  of the flow paths  31  and  32 . However, the present invention is not limited to this configuration, as long as it has a configuration of comprising each accommodation portion and each flow path therein. 
     The test container  2  has a structure of the first flow path  31  and the second flow path  32  in which a water contact angle R 1  of the inner surface of the first flow path  31  is set to be greater than a water contact angle R 2  of the inner surface of the second flow path  32 , as the liquid return prevention structure. In this example, a hydrophobic surface  34  obtained by performing a hydrophobic treatment is formed on the inner surface of the first flow path  31 . 
     In order to generate a difference in a water contact angle between the inner surface of the first flow path  31  and the inner surface of the second flow path  32 , the hydrophobic treatment may be performed on the inner surface of the first flow path  31  as in this example and/or a hydrophilic treatment may be performed on the inner surface of the second flow path  32 . 
     The test container  2  comprises the first flow path  31  at the upper end position of the first accommodation portion  21  and the second accommodation portion  22 , and the second flow path  32  at the upper end position of the second accommodation portion  22  and the third accommodation portion  23 , respectively. Accordingly, the liquid accommodated in the accommodation portion is difficult to flow into the flow path, compared to a case where the flow path is comprised at a lower end or in the middle in a depth direction. Therefore, it is possible to prevent a passage of the liquid into the flow path due to a capillary phenomenon or the like without applying an external force. Meanwhile, since the portion  14 A deformable toward the inside of the second accommodation portion  22  is comprised at the upper portion of the second accommodation portion  22 , the portion  14 A is deformed toward the inside of the second accommodation portion  22  to reduce a volume of the second accommodation portion  22 , thereby easily realizing liquid feeding to the third accommodation portion  23  by pushing the liquid accommodated in the second accommodation portion  22 . 
     The portion  14 A of the container main body  10 B is deformed in the direction toward the second accommodation portion  22 , so that the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the second flow path  32 . In this case, since the water contact angle of the inner surface of the first flow path  31  is greater than the water contact angle of the inner surface of the second flow path  32 , the liquid pushed from the second accommodation portion  22  is preferentially fed to the second flow path  32  having a smaller water contact angle. Accordingly, the liquid return to the first flow path  31  can be suppressed, and the liquid feeding properties to the third accommodation portion  23  at a downstream side is high. According to this configuration, it is possible to suppress the liquid return to the first flow path  31  and increase the liquid feeding properties to the third accommodation portion  23  with a simple process of only the surface treatment. 
     The surface treatment such as the hydrophilic treatment or the hydrophobic treatment is preferably formed on the entire inner surface of each flow path, but a part of the inner surface may not be treated. 
     Examples of the hydrophilic treatment include a surface modification treatment such as a corona treatment, a plasma treatment, an ozone treatment, a treatment of applying a hydrophilic coating agent, and bonding of a hydrophilic film. Examples of the hydrophobic treatment include a treatment of applying a hydrophobic coating agent such as a fluororesin or a hydrophobic silica-containing resin, a silane coupling treatment, and bonding of a water-repellent film. 
     A difference R 1 −R 2  between the water contact angle R 1  of the first flow path  31  and the water contact angle R 2  of the second flow path  32  is preferably 10° or more, more preferably 20° or more, even more preferably 40° or more, and further preferably 60° or more. 
     In the present specification, the water contact angle is a contact angle of pure water. Specifically, 1 μL of pure water is added dropwise to the inner surface of the flow path and the accommodation portion under the condition of an atmosphere temperature of 25° C., the contact angle is measured by the θ/2 method using a fully-automatic contact angle meter (model number: DM-701, Kyowa Interface Science Co., Ltd.), and an arithmetic mean value of values obtained by measuring 5 times is used. 
     Test Container  3   
     The test container  3  will be described.  FIG. 15  is an exploded perspective view showing a schematic configuration of a test container  3 .  FIG. 16  is an cross-sectional view showing a schematic configuration of the test container  3 .  FIG. 17  is a plan view showing a schematic configuration of a main body portion  12 C of the test container  3 . 
     The test container  3  shown in  FIGS. 15, 16, and 17  comprises the container main body  10 C including a first accommodation portion  21 , a second accommodation portion  22 , and a third accommodation portion  23  each accommodating a liquid, a first flow path  31  connecting the first accommodation portion  21  and the second accommodation portion  22  to each other at respective upper end positions thereof, and a second flow path  32  connecting the second accommodation portion  22  and the third accommodation portion  23  to each other at respective upper end positions thereof. The container main body  10 C has at least the portion  14 A forming the upper wall surface  22   b  of the second accommodation portion  22  having flexibility to be deformable inwards of the second accommodation portion  22 . In a case of applying to the liquid feeding device  202 , the portion  14 B forming the upper wall surface  31   b  of the first flow path  31  further has flexibility deformable toward the inside of the first flow path  31 . 
     In this example, the container main body  10 C comprises the main body portion  12 C and the upper lid member  14 . The main body portion  12 C has an opening in a portion forming each of the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23 . The container main body  10 C has a configuration in which the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23  are formed therein by covering the opening of the main body portion  12 C with the upper lid member  14 . That is, the main body portion  12 C constitutes the inner bottom surfaces  21   a  to  23   a  and the side wall surfaces of the accommodation portions  21  to  23 , and the inner bottom surfaces  31   a  and  32   a  and the side wall surfaces of the flow paths  31  and  32 , respectively. The upper lid member  14  configures the upper wall surfaces  21   b  to  23   b  of the accommodation portions  21  to  23  and the upper wall surfaces  31   b  and  32   b  of the flow paths  31  and  32 . However, the present invention is not limited to this configuration, as long as it has a configuration of comprising each accommodation portion and each flow path therein. 
     The test container  3  has a structure of a stepped portion  40  which is provided on the second accommodation portion  22  side of the first flow path  31  and which includes two or more steps  41  and  42  from the inner bottom surface  22   a  of the second accommodation portion  22 , as the liquid return prevention structure. On the other hand, the second flow path  32  does not comprise a stepped portion. In addition, in this example, the stepped portion is provided on the first accommodation portion  21  side of the first flow path  31 , but the stepped portion may not be provided on the first accommodation portion  21  side. 
     The test container  3  comprises the first flow path  31  at the upper end position of the first accommodation portion  21  and the second accommodation portion  22 , and the second flow path  32  at the upper end position of the second accommodation portion  22  and the third accommodation portion  23 , respectively. Accordingly, the liquid accommodated in the accommodation portion is difficult to flow into the flow path, compared to a case where the flow path is comprised at a lower end or in the middle in a depth direction. Therefore, it is possible to prevent a passage of the liquid into the flow path due to a capillary phenomenon or the like without applying an external force. Meanwhile, since the portion  14 A deformable toward the inside of the second accommodation portion  22  is comprised at the upper portion of the second accommodation portion  22 , the portion  14 A is deformed toward the inside of the second accommodation portion  22  to reduce a volume of the second accommodation portion  22 , thereby easily realizing liquid feeding to the third accommodation portion  23  by pushing the liquid accommodated in the second accommodation portion  22 . 
     The portion  14 A of the container main body  10 C is deformed in the direction toward the second accommodation portion  22 , so that the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the second flow path  32 . In this case, since the first flow path  31  comprises the stepped portion  40  having two or more steps, a barrier in a case where the liquid accommodated in the second accommodation portion  22  passes through the first flow path  31  has two or more steps. Accordingly, the invasion of the liquid into the first flow path  31  is suppressed, and the liquid pushed out from the second accommodation portion  22  is preferentially fed to the second flow path  32  having a smaller barrier. Therefore, the liquid return to the first flow path  31  is suppressed, and the liquid feeding properties to the third accommodation portion  23  at a downstream side is high. It is possible to obtain a high effect of preventing the liquid return to the first flow path  31  by providing the stepped portion  40  in the first flow path  31 . 
     The stepped portion  40  includes a first step  41  on the second accommodation portion  22  side and a second step  42 . The stepped portion  40  is not limited to two steps and may have three steps or four or more steps. However, from a viewpoint of avoiding complication of the structure, the stepped portion  40  preferably has two or three steps. 
     The height h 1  of the first step  41  is preferably 25% or more, more preferably 30% or more, and even more preferably 50% or more of d, where d is a height (depth) from the inner bottom surface  22   a  to the upper wall surface  22   b  of the second accommodation portion  22 . 
     A height h 12  of the second step  42  is preferably 50% or more, more preferably 60% or more, and even more preferably 80% or more of the height d of the second accommodation portion  22 . A difference between the height h 12  of the second step  42  and the height h 1  of the first step  41  is preferably 20% or more of the height h 1  of the first step  41 , from a viewpoint of preventing the liquid return. The height h 12  of the second step  42  is defined as a height from the inner bottom surface  22   a  of the second accommodation portion  22  at the corner of the level difference portion with the first step  41 . 
     Test Container  3 A 
       FIG. 18  shows a test container  3 A of a modification example of the third embodiment. The test container  3 A comprises a container main body  10 D consisting of a main body portion  12 D and the upper lid member  14 . In the test container  3 A, a corner (here, a corner  43 ) formed by the inner bottom surface and the inner side surface forming at least one step of the stepped portion  40  has an acute angle. By setting the corner  43  of the level difference portion to have an acute angle, it is possible to more effectively suppress the flow of the liquid accommodated in the second accommodation portion  22  to the first flow path  31 , compared to a case where the angle is equal to or greater than 90°. Therefore, it is possible to more preferentially feed the liquid accommodated in the second accommodation portion  22  to the second flow path  32 . 
     In the test container  3 A shown in  FIG. 18 , only the corner  43  of the second step  42  of the stepped portion  40  has an acute angle, but corners of all of the steps included in the flow path  31  may have an acute angle. In a case where at least one corner of the steps  41  and  42  of the stepped portion  40  is an acute angle, the effect of suppressing the flow of the liquid from the second accommodation portion  22  into the first flow path  31  is improved. 
     The liquid feeding method of the liquid in the present test container  3  or the modification example thereof is the same as that in the case of the test container  1  of the first embodiment. 
     As described above, the test container  1  comprises a structure in which the height h 1  of the first flow path  31  is higher than the height h 2  of the second flow path  32  (hereinafter, referred to as a liquid return prevention structure  1 ). The test container  2  comprises a structure of the first flow path  31  and the second flow path  32  in which the water contact angle of the inner surface of the first flow path  31  is set to be greater than the water contact angle of the inner surface of the second flow path  32  (hereinafter, referred to as a liquid return prevention structure  2 ). The test container  3  has a structure of the stepped portion  40  including two or more steps from the inner bottom surface  22   a  of the second accommodation portion  22  configured on the second accommodation portion side of the first flow path  31  (hereinafter, referred to as a liquid return prevention structure  3 ). 
     It is also preferable to comprise these liquid return prevention structures  1  to  3  in combination. For example, as shown in  FIG. 19 , a test container  4  comprising the liquid return prevention structure  1  and the liquid return prevention structure  2  may be used. The test container  4  comprises a container main body  10 E formed of a main body portion  12 E and the upper lid member  14 . The test container  4  has a structure in which the height h 1  of the first flow path and the height h 2  of the second flow path satisfy a relationship of h 1 &gt;h 2  and comprises the hydrophobic surface  34  obtained by performing a hydrophobic treatment on the inner surface of the first flow path  31 , and the water contact angle of the inner surface of the first flow path  31  is higher than the water contact angle of the inner surface of the second flow path  32 . 
     For example, as shown in  FIG. 20 , a test container  5  comprising the liquid return prevention structure  2  and the liquid return prevention structure  3  may be used. The test container  5  comprises a container main body  10 F forming of a main body portion  12 F and the upper lid member  14 . The test container  5  comprises the hydrophobic surface  34  obtained by performing a hydrophobic treatment on the inner surface of the first flow path  31  and comprises the stepped portion  40  in the first flow path  31 , and the water contact angle of the inner surface of the first flow path  31  is higher than the water contact angle of the inner surface of the second flow path  32 . 
     In addition, a test container comprising the liquid return prevention structure  1  and the liquid return prevention structure  3  may be used, or, as shown in  FIG. 21 , a test container  6  comprising all of the liquid return prevention structures  1  to  3  may be used. The test container  6  comprises a container main body  10 G formed of a main body portion  12 G and the upper lid member  14 . The test container  6  has a structure in which the height h 1  of the first flow path  31  and the height h 2  of the second flow path  32  satisfy a relationship of h 1 &gt;h 2  and comprises the hydrophobic surface  34  obtained by performing a hydrophobic treatment on the inner surface of the first flow path  31 , and the water contact angle of the inner surface of the first flow path  31  is higher than the water contact angle of the inner surface of the second flow path  32 . In addition, the first flow path  31  comprises the stepped portion  40 . 
     According to the test container comprising two or three the liquid return prevention structures  1  to  3  in combination, it is possible to obtain a higher effect of the liquid return prevention, compared to a case of comprising only the liquid return prevention structure  1 , only the liquid return prevention structure  2 , or only the liquid return prevention structure  3 . 
     In addition, in the test container of the present disclosure, the liquid return prevention structure is not limited to the above example, and the first flow path between the second accommodation portion and the first accommodation portion may have a structure in which the liquid accommodated in the second accommodation portion relatively hardly flows, compared to the second flow path between the second accommodation portion and the third accommodation portion. For example, a structure including a valve may be comprised in each of the first flow path and the second flow path may be provided as the liquid return prevention structure. In a case where a valve is provided in each of the first flow path and the second flow path, the liquid is fed in a state where the valve of the first flow path is closed and valve of the second flow path is opened, in a case of feeding the liquid from the second accommodation portion to the third accommodation portion, it is possible to effectively prevent the liquid return to the first accommodation portion and improve the liquid feeding properties to the third accommodation portion. 
     Nucleic Acid Extraction Test Device 
     The liquid feeding device of the technology of the present disclosure can be applied to, for example, a nucleic acid extraction test device. A nucleic acid extraction test device and a nucleic acid extraction test as an embodiment of the liquid feeding device of the present disclosure will be described. 
       FIG. 22  is a configuration diagram showing a schematic configuration of a nucleic acid extraction test device  100  comprising the test container  101 . The nucleic acid extraction test device  100  comprises the test container  101 , the pressing machine  50 , a dispenser  106 , a magnetic field generation and movement unit  107 , and a transportation portion  102  for the test container  101 . 
       FIG. 23  is an exploded perspective view of a test container  101  and a diagram showing a main part of a dispenser  106 .  FIG. 24  is a diagram showing the test container  101  and a magnet M of the magnetic field generation and movement unit  107 .  FIG. 25  is a diagram showing the test container  101  and a main part of the pressing machine  50 .  FIGS. 24 and 25  show cross-sectional views taken along a line  18 - 18  of the test container  101  shown in  FIG. 23 . 
     The test container  101  comprises a container main body  110  comprising four accommodation portions  120  to  123  accommodating a liquid, respectively, a chromatographic carrier accommodation portion  125  accommodating a chromatographic carrier  128 , and four flow paths  130 ,  131 ,  132 , and  135  therein. 
     The container main body  110  comprises a main body portion  112  and an upper lid member  114 . The main body portion  112  has an opening in a portion forming each of the accommodation portions  120  to  123  and  125  and the flow paths  130 ,  131 ,  132 , and  135 . The container main body  110  has a configuration in which the accommodation portions  120  to  123  and  125  and the flow paths  130 ,  131 ,  132 , and  135  are formed therein by covering the main body portion  112  with the upper lid member  114 . The main body portion  112  configures the side wall surface and the bottom surface of each of the accommodation portions and the flow paths, and the upper lid member  114  configures the upper wall surface of each of the accommodation portions and the flow paths. In this example, the upper lid member  114  is formed of a flexible film. The upper lid member  114  is provided with an injection port (not shown) for injecting the liquid accommodated in each of the accommodation portions  120  to  123 . The tips of syringes  160  to  163  are inserted into the injection ports, respectively, and various liquids can be injected into the corresponding accommodation portions  120  to  123 . 
     The accommodation portion  120  is a magnetic particle collecting chamber (hereinafter, referred to as the magnetism collecting chamber  120 ) which accommodates a specimen solution  150  containing magnetic particles P to which a nucleic acid is adsorbed. The accommodation portion  121  is a cleaning chamber (hereinafter, referred to as a cleaning chamber  121 ) which accommodates a cleaning solution  151  and cleans a substance non-specifically adsorbed to the magnetic particles P. The accommodation portion  122  is a PCR chamber (hereinafter, referred to as a PCR chamber  122 ) which accommodates a polymerase chain reaction (PCR) solution  152 . The accommodation portion  123  is a detection chamber (hereinafter, referred to as a detection chamber  123 ) for mixing an amplified nucleic acid and a development solution  153 . 
     The flow path  130  connects a magnetism collecting chamber  120  and the cleaning chamber  121  to each other at an upper end position. The flow path  130  comprises a stepped portion on the sides of the magnetism collecting chamber  120  and the cleaning chamber  121 , to suppress the flow of the specimen solution  150  accommodated in the magnetism collecting chamber  120  to the flow path  130  and to prevent the mixing of the specimen solution  150  with the cleaning solution  151  accommodated in the cleaning chamber  121 . 
     The flow path  131  connects the cleaning chamber  121  and the PCR chamber  122  to each other at an upper end position and the flow path  132  connects the PCR chamber  122  and the detection chamber  123  to each other at an upper end position. The cleaning chamber  121 , the PCR chamber  122 , the detection chamber  123 , and the flow paths  131  and  132  correspond to the first accommodation portion, the second accommodation portion, the third accommodation portion, the first flow path, and the second flow path in the technology of the present disclosure, respectively. In addition, here, the liquid return prevention structure of suppressing the backflow of the liquid to the cleaning chamber  121 , in a case of feeding the liquid accommodated in the PCR chamber  122  to the detection chamber  123  through the flow path  132  may be comprised. In this example, the liquid return prevention structure  3  is included as the liquid return prevention structure. That is, as the liquid return prevention structure, a structure of a stepped portion including two or more steps from an inner bottom surface  122   a  of the PCR chamber  122 , which is formed on the PCR chamber  122  side of the flow path  131 , is comprised. 
     The liquid return prevention structure may include a structure (liquid return prevention structure  1 ) in which a height of the first flow path (flow path  131 ) is higher than a height of the second flow path (flow path  132 ). In addition, a structure of the first flow path and the second flow path in which the water contact angle of the inner surface of the first flow path is set to be greater than the water contact angle of the inner surface of the second flow path (liquid return prevention structure  2 ) may be included. Alternatively, two or more of other liquid return prevention structures and liquid return prevention structures  1  to  3  may be provided in combination. 
     The flow path  132  connects the PCR chamber  122  and the detection chamber  123  at the upper end position. The flow path  132  may comprise a valve (not shown), in order to prevent evaporation of the liquid in a case of adjusting a temperature of the PCR chamber. The valve may be any valve that can be opened in a case where liquid is fed from the PCR chamber  122  to the detection chamber  123 . 
     The flow path  135  connects the detection chamber  123  and the chromatographic carrier accommodation portion  125  to each other at a lower end position. 
     The magnetic particles P are particles that are attracted by magnetic force. The magnetic particles P are, for example, magnetic particles processed so as to adsorb a specific sample such as DNA. Specifically, as the magnetic particles P, model number: Magnosphere MX100/Carboxyl and model number: Magnosphere MS160/Tosyl manufactured by JSR Corporation, sicastar manufactured by Corefront, Magrapid manufactured by Sanyo Chemical Industries, Ltd. can be used. 
     As the magnetic particles P, magnetic particles having a particle size in a range of 0.01 μm to 100 μm are used. As the magnetic particles P, magnetic particles having a particle size of approximately 1 μm to 10 μm are preferably used. The magnetic particles P may be comprised in the magnetism collecting chamber  120  in advance, or may be injected into the magnetism collecting chamber  120  together with the specimen solution  150 . 
     The specimen solution  150  is, for example, a specimen solution containing a nucleic acid extracted from a specimen. The specimen solution  150  may include a surfactant for extracting a nucleic acid such as deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from the specimen and adsorbing the nucleic acid on the surfaces of the magnetic particles P. In addition, as the surfactant, for example, sodium dodecyl sulfate, polyoxyethylene sorbitan monolaurate (Tween 20), Triton X-100, or the like can be used. These surfactants may be used alone or in combination of a plurality thereof. A chaotropic substance such as guanidine hydrochloride may be included in order to promote extraction of nucleic acid from the specimen and surface adsorption to the magnetic particles P. In addition, instead of containing the surfactant, a nucleic acid extracted from a specimen using a column may be contained. In addition, a surfactant for suppressing aggregation of the magnetic particles P may be included. 
     The cleaning solution  151  removes the substance non-specifically adsorbed to the magnetic particles P. As the cleaning solution  151 , water or a buffer solution, an organic solvent such as ethanol and isopropyl alcohol, or the like can be used. In a case where the buffer solution is used as the cleaning solution, salt is not particularly limited, but salt of tris or phosphoric acid is preferably used. In addition, in order to suppress the elution of RNA in the cleaning step, the surfactant such as sodium dodecyl sulfate, Triton X-100, or the like may be contained. 
     The PCR solution  152  is a solution for performing a process for amplifying nucleic acid by PCR. The PCR solution  152  contains, for example, reverse transcriptase, dNTP in which four kinds of deoxyribonucleotide triphosphates are mixed, and a primer for reverse transcriptase. Transcriptase is an enzyme that synthesizes complementary deoxyribonucleic acid (cDNA) using a base sequence of RNA as a template. 
     The chromatographic carrier accommodation portion  125  accommodates the chromatographic carrier  128 . In the chromatographic carrier accommodation portion  125 , the development solution  153  containing the amplified nucleic acid is developed. The chromatographic carrier  128  is a nucleic acid chromatographic carrier and indicates whether or not the target nucleic acid is present in the development solution  153 . 
     The dispenser  106  comprises the syringes  160  to  163  for adding various liquids  150  to  153  to the respective accommodation portions  120  to  123  of the test container  101 . 
     The pressing machine  50  comprises a plunger  52  is configured to be able to press a region corresponding to the PCR chamber  122  of the container main body  110  (here, the upper lid member  114 ) by the plunger  52 . 
     The magnetic field generation and movement unit  107  includes the magnet M and a movement mechanism  170  that moves the magnet M. 
     The magnet M is, for example, a permanent magnet, but may be an electromagnet. As shown in  FIG. 24 , the magnet M is freely moved between positions A 0  to A 5  of the test container  101  on the upper lid member  114 . The positions A 0 , A 3 , and A 5  are positions where a magnetic force does not act on the magnetic particles P accommodated in the test container  101 , even in a case where the magnet M is disposed. The position A 1  is a position on the magnetism collecting chamber  120  and is a position where a magnetic force acts on the magnetic particles P in the magnetism collecting chamber  120  in a case where the magnet M is disposed. The position A 2  is a position on the cleaning chamber  121  and is a position where magnetic force acts on the magnetic particles P in the cleaning chamber  121  in a case where the magnet M is disposed. The position A 4  is a position on the PCR chamber  122  and is a position where a magnetic force acts on the magnetic particles P in the PCR chamber  122  in a case where the magnet M is disposed. 
     In a case of moving the magnetic particles P from the magnetism collecting chamber  120  to the cleaning chamber  121 , first, the magnet M is disposed at the position A 1 . In a case where the magnet M is disposed at the position A 1 , the magnetic particles P accommodated in the magnetism collecting chamber  120  are collected by the magnetic force of the magnet M and are attracted and collected at the position corresponding to the magnet M with the upper lid member  14  interposed therebetween. In a case where the magnet M is moved to the position A 2  along the upper lid member  14  from this state, the magnetic particles P are separated from the specimen solution  150  and moved to the cleaning chamber  121  according to the movement of the magnet M. Then, in a case where the magnet M is moved to the position A 3 , the magnetic particles P are dispersed in the cleaning solution  151 . 
     In the same manner, in a case of moving the magnetic particles P from the cleaning chamber  121  to the PCR chamber  122 , first, the magnet M is disposed at the position A 2 . In a case where the magnet M is disposed at the position A 2 , the magnetic particles P accommodated in the cleaning chamber  121  are attracted and collected at the position corresponding to the magnet M with the upper lid member  14  interposed therebetween. In a case where the magnet M is moved to the position A 4  along the upper lid member  14  from this state, the magnetic particles P are separated from the cleaning solution  151  and moved to the PCR chamber  122  along the movement of the magnet M. After that, in a case where the magnet M is moved to the position A 5 , the magnetic particles P are dispersed in the PCR solution  152 . 
     The movement mechanism  170  has a function of allowing the magnet M to pass the upper portion of the flow path  130  from the position A 1  on the magnetism collecting chamber  120 , to pass the upper portion of the flow path  131  from the position A 2  on the cleaning chamber  121 , and to freely move to the position A 4  on the PCR chamber  122 . In addition, the movement mechanism  170  moves the magnet M to the positions A 0 , A 3  and A 5  where the magnetic force does not reach the inside of the chambers  120 ,  121  and  122 . 
     The nucleic acid extraction test device  100  further comprises a temperature control unit  108  (see  FIG. 24 ). The temperature control unit  108  controls a temperature of the PCR solution in the PCR chamber  122 . The temperature control unit  108  comprises a heating unit such as a heater or a Peltier element for heating a solution, and a cooling unit such as a Peltier element, a fan, a heat sink, or a liquid cooling mechanism for cooling a solution. The temperature control unit  108  raises or lowers the temperature of the solution so that the temperature is adjusted to a suitable temperature in each step of a heat denaturation step, an annealing step, and an extension step in PCR. 
     A transportation unit  102  is a device that relatively moves the test container  101  relatively to the dispenser  106 , the magnetic field generation and movement unit  107 , and the pressing machine  50 . The transportation unit  102  may transport only the test container  101 , or move the respective positions of the dispenser  106 , the magnetic field generation and movement unit  107 , and the pressing machine  50  with respect to the test container  101 . 
     Nucleic Acid Extraction Test Method 
     The steps of the nucleic acid extraction test in the nucleic acid extraction test device  100  comprising the test container  101  will be described. 
     Pretreatment (Adsorption Process) 
     A sample containing RNA is mixed with a solution containing a surfactant that dissolves a cell membrane and the magnetic particles P to adsorb the RNA to the magnetic particles P. The sample containing RNA is not particularly limited, as long as it contains the RNA such as a biological sample and virus. As necessary, impurities may be removed with a filter or the like. 
     Magnetization Collection Process 
     The specimen solution  150  containing the magnetic particles P having RNA adsorbed, which was obtained in the pretreatment, is injected into the magnetism collecting chamber  120  by the syringe  160 . After that, the magnet M is set at the position A 1  on the magnetism collecting chamber  120 . Accordingly, the magnetic particles P accommodated in the magnetism collecting chamber  120  are attracted to the magnet M and are collected at a position corresponding to the magnet M on the upper surface to be in an aggregated state (see  FIG. 24 ). 
     In the magnetism collecting chamber  120 , the adsorption process and the magnetism collection process may be performed in time series. 
     Then, by moving the magnet M along the flow path  130 , the magnetic particles P are separated from the specimen solution  150  and moved to the cleaning chamber  121 . 
     Cleaning Step 
     In the cleaning chamber  121 , the magnetic particles P adsorbed with RNA are cleaned with the cleaning solution  151  accommodated in the cleaning chamber  121 . The cleaning chamber  121  may be filled with the cleaning solution  151  in advance, or the cleaning solution  151  may be injected after the magnetic particles P are moved. The magnet M is moved to the position (position A 3 ) where the magnetic force does not affect the cleaning chamber  121  and the magnetic particles P are dispersed in the cleaning solution  151 , thereby promoting the cleaning. By performing the cleaning, the substances other than RNA that are non-specifically bound to the magnetic particles P are removed. 
     Then, by returning the magnet M to the position A 2  on the cleaning chamber  121 , the magnetic particles P are collected again at the position corresponding to the magnet M on the upper surface, and the magnet M is moved to the position A 4  on the PCR chamber  122  along the flow path  131 . Thereby the magnetic particles P are separated from the cleaning solution  151  and are moved to the PCR chamber  122 . After that, the magnet M is moved to the position A 5  where the magnetic force does not affect the PCR chamber  122 , so that the magnetic particles P are dispersed in the PCR solution  152 . 
     PCR Process 
     In the PCR chamber  122 , the RNA adsorbed to the magnetic particles P is eluted into the PCR solution  152 , and the DNA amplification by PCR is performed. The cDNA is synthesized from the extracted RNA and the cDNA is amplified by PCR. In this case, the magnetic particles P sink to the inner bottom surface of the PCR chamber  122  due to gravity. 
     Liquid Feeding Process 
     After the PCR step, the solution containing the amplified cDNA in the PCR chamber  122  is fed to the detection chamber  123 . The test container  101  comprises the flow path  131  at the upper end position of the cleaning chamber  121  and the PCR chamber  122 , and the flow path  132  at the upper end position of the PCR chamber  122  and the detection chamber  123 , respectively. Accordingly, it is possible to prevent the passage of the solution  152  from the PCR chamber  122  to the flow paths  131  and  132  due to a capillary phenomenon or the like, before this liquid feeding process. 
     As shown in  FIG. 25 , in a case where the liquid is fed, the plunger  52  is positioned on the PCR chamber  122  and the plunger  52  is pushed down along the cylinder  54 . The flexible upper lid member  114  is pushed by the plunger  52  and pushed inwards of the PCR chamber  122 . This reduces the volume of the PCR chamber  122 , so that the liquid in the PCR chamber  122  is fed to the detection chamber  123  through the flow path. In this case, since the return prevention structure is provided, most of the solution  152  in the PCR chamber  122  does not flow backward to the cleaning chamber  121  side, and a large amount of the solution extruded from the PCR chamber  122  can be fed to the detection chamber  123 . In addition, since the flow path  132  is comprised at the upper end position of the PCR chamber  122 , a supernatant portion of the PCR solution can be preferentially fed while the magnetic particles P are submerged on the inner bottom surface, and the magnetic particles P can be suppressed from flowing out to the detection chamber  123  side. By suppressing the magnetic particles P from flowing to the detection chamber  123 , it is possible to perform a test with less noise in the next step. 
     Detection Process 
     In the detection chamber  123 , the solution containing cDNA is mixed with the development solution. After that, the mixed liquid passes through the flow path  135  and is developed by the nucleic acid chromatographic carrier (chromatographic carrier  128 ) disposed in the chromatographic carrier accommodation portion  125 . In a case where the RNA to be tested is contained, a positive result is obtained, and in a case where not, a negative result is obtained. 
     The nucleic acid extraction test is performed as described above. 
     Hereinabove, the case where the reverse transcription PCR method is used as the amplification method has been described, but the amplification method is not limited to the reverse transcription PCR method, and well-known amplification methods such as the transcription PCR method, the isothermal amplification method (for example, Nucleic Acid Sequence-Based Amplification (NASBA), Loop-mediated Isothermal Amplification (LAMP), transcription-reverse transcription concerted (TRC), and the like) can be used. In addition, hereinabove, the case where the nucleic acid chromatography method is used as the detection method has been described above, but the detection method is not limited to the nucleic acid chromatography method, and well-known methods such as a fluorescence detection method (intercalator method, probe method, or the like), a light scattering method using gold nanoparticles, a sequence method, an electrochemical method, a piezoelectric method, and detection of a weight or a mechanical change can be used. In these cases, the container does not necessarily comprise the chromatographic carrier  128  and the accommodation portion  125  thereof. On the other hand, the test device may comprise a detection unit suitable for various detection methods of a fluorescence detection unit and the like for detecting fluorescence from the detection chamber  123 . However, the nucleic acid chromatography method is preferable because a high-priced detection system and detection equipment are not necessary and the operation in the analysis is simple. 
     By using the test container  101 , the solution containing the DNA amplified in the PCR chamber  122  can be efficiently fed to the detection chamber  123  while suppressing the backflow to the cleaning chamber  121 , and a sufficient amount of solution to be fed can be realized. Since the backflow can be suppressed to increase the amount of liquid to be fed to the detection chamber  123 , a total amount of DNA that flows into the detection chamber  123  can be increased, which leads to improvement in determination accuracy. 
     In regard to the test container  101 , a set of the test container  101 , the magnetic particles P, and various treatment liquids such as the cleaning solution  151 , the PCR solution  152 , and the development solution  153  can also be provided as a test kit. The test kit may further include other treatment liquid such as a nucleic acid eluate. In addition, as the test kit, it is also possible to provide a set of only the test container  101  and the magnetic particles P. The magnetic particles P may be set in the magnetism collecting chamber  120  of the test container  101  in advance, or may be separately prepared. 
     The technology of the present disclosure is not limited to the embodiment described above, and various modifications, changes, and improvements can be made without departing from the spirit of the invention. For example, the modification examples described above may be appropriately configured in combination. 
     EXAMPLES 
     Hereinafter, more specific examples and comparative examples of the technology of the present disclosure will be described. 
     Test containers A to D having the same shape as the test container  201  comprising the three accommodation portions and the flow paths connecting the three accommodation portions shown in  FIGS. 1 to 4  were made, and the evaluation of the liquid feeding method was performed with respect to the examples and the comparative examples in which the pressing point and the pressing method in a case of feeding the liquid were changed. The test containers A to D (test container  201 ) comprise the first accommodation portion  221 , the second accommodation portion  222 , the third accommodation portion  223 , the first flow path  231  connecting the first accommodation portion  221  and the second accommodation portion  222  at the upper end, and the second flow path  232  connecting the second accommodation portion  222  and the third accommodation portion  223  at the upper end. The three accommodation portions  221 ,  222 , and  223  had the same shape, and had a length L of 7.5 mm, a width W of 7.5 mm, and a depth d of 1 mm. A width of the first flow path  231  and the second flow path  232  was 1 mm. In addition, a height h of each of the first flow path  231  and the second flow path  232  from the inner bottom surface  222   a  of the second accommodation portion  222  was 0.8 mm. 
     First, the test containers A to D were manufactured as follows. 
     Test Container A 
     The same test container A was used in Examples 1 to 6 and Comparative Examples 1 and 2. 
     The test container A was formed of the container main body  212  and the upper lid member  214 , and the container main body  212  was formed of a main body portion forming the side wall surfaces of the first accommodation portion  221 , the second accommodation portion  222 , and the flow path  230 , and a bottom surface member forming the inner bottom surfaces of the first accommodation portion  221 , the second accommodation portion  222 , and the flow path  230 . 
     Polycarbonate (PC) was used as the material of the main body portion  210 . Specifically, the main body portion was injection-molded using IUPILON EB-3001R manufactured by Mitsubishi Engineering Plastics Co., Ltd. As the bottom surface member, Technoloy C000 (thickness of 100 μm) manufactured by Sumika Acrylic Sales Co., Ltd. was used. In addition, a silicone film GFSX6000 (thickness of 300 μm) manufactured by Tomita Mateqs Co., Ltd. was used for the upper lid member  214 . 
     The bottom surface member was roller-bonded to the bottom surface of the main body portion using an adhesive #9969 manufactured by  3 M Japan Co., Ltd., and the upper lid member  214  was roller-bonded to the upper surface of the main body portion using a silicone adhesive NSD-50 manufactured by Nipper Co., Ltd. to obtain a test container A. 
     Test Container B 
     A test container B was used in Example 7. The test container B had the same shape as the test container A, except that polypropylene (PP) was used as the material of the main body  210 . 
     The main body portion was injection-molded using WINTEC WMG03UX manufactured by Japan Polypro Corporation. As the bottom surface member, Trefan BO60-2500 (thickness of 60 μm) manufactured by Toray Industries, Inc. was used. In addition, in the same manner as in Example 1, a silicone film GFSX6000 (thickness of 300 μm) manufactured by Tomita Mateqs Co., Ltd. was used for the upper lid member  214 . 
     Test Container C 
     A test container C was used in Example 8. The test container C had the same shape as the test container A, except that polymethyl methacrylate resin (PMMA) was used as the material of the main body  210 . 
     The main body portion was injection-molded using Acrypet VH001 manufactured by Mitsubishi Chemical Corporation, and Technoloy 5001 manufactured by Sumika Acrylic Sales Co., Ltd. (thickness 125 μm) was used as the bottom surface member. In addition, in the same manner as in Example 1, a silicone film GFSX6000 (thickness of 300 μm) manufactured by Tomita Mateqs Co., Ltd. was used for the upper lid member  214 . 
     Test Container D 
     A test container D was used in Example 9. The test container D had the same shape as the test container A, except that COP was used as the material of the main body  210 . 
     The main body portion was injection-molded using ARTON F4520 manufactured by JSR Corporation, and a film having a thickness of 50 μm obtained by forming a film of ARTON R5000 manufactured by JSR Corporation was used as the bottom surface member. In addition, in the same manner as in Example 1, a silicone film GFSX6000 (thickness of 300 μm) manufactured by Tomita Mateqs Co., Ltd. was used for the upper lid member  214 . 
     Evaluation of Liquid Feeding Properties 
     Using the test containers A to D, liquid feeding was performed by the methods of Examples 1 to 9 and Comparative Examples 1 and 2 below, and the liquid feeding properties were evaluated. 
     As shown in  FIG. 26 , a pushing position of the portion  214 A on the upper portion of the first accommodation portion  222  is shown as a position in a case where the boundary C 1  between the second accommodation portion  222  and the first flow path  231  is 0% position and the boundary C 2  between the second accommodation portion  222  and the third flow path  232  is 100% position. In addition, the pushing position of the portion  214 B on the upper portion of the first flow path  231  is shown as a position in a case where the boundary C 0  between the first flow path  231  and the first accommodation portion  221  is 0% position and the boundary C 1  between the first flow path  231  and the second accommodation portion  222  is 100% position. 
     After filling the second accommodation portion  222  with water, the liquid was fed by the method of each example and comparative example, a weight of the liquid fed to the third accommodation portion  223  and a weight of the liquid flowed back to the first accommodation portion  221  were measured, and a ratio thereof was calculated as a liquid return rate. 
     That is, 
       Liquid return rate=(Weight of liquid returned to the first accommodation portion [mg])/(Weight of liquid fed to the third accommodation portion [mg]). 
     The liquid return rate was evaluated according to the following criteria. For practical use, E or higher is required. In addition, practically, D or higher is preferable, C or higher is more preferable, and B or higher is further preferable. 
     A: less than 5% 
     B: 5% or more and less than 10% 
     C: 10% or more and less than 15% 
     D: 15% or more and less than 20% 
     E: 20% or more and less than 25% 
     F: 30% or more 
     Example 1 
     Using the test container A, a 30% position of the portion  214 A on the second accommodation portion  222  was pressed by a ball plunger to carry out liquid feeding (see  FIG. 5 ). 
     Example 2 
     Using the test container A, a 15% position of the portion  214 A on the second accommodation portion  222  was pressed by a ball plunger to carry out liquid feeding (see  FIG. 5 ). 
     Example 3 
     Using the test container A, a 25% position of the portion  214 B on the first flow path  231  and a 50% position of the portion  214 A on the second accommodation portion  222  were pressed by a ball plunger to carry out liquid feeding (see  FIG. 7 ). In this case, the portions  214 A and  214 B were pressed at the same time. 
     Example 4 
     Using the test container A, a 50% position of the portion  214 B on the first flow path  231  and a 50% position of the portion  214 A on the second accommodation portion  222  were pressed by a ball plunger to carry out liquid feeding (see  FIG. 7 ). In this case, the portions  214 A and  214 B were pressed at the same time. 
     Example 5 
     Using the test container A, a 75% position of the portion  214 B on the first flow path  231  and a 50% position of the portion  214 A on the second accommodation portion  222  were pressed by a ball plunger to carry out liquid feeding (see  FIG. 7 ). In this case, the portion  214 B on the first flow path was pressed in first, and then the portion  214 A on the second accommodation portion  222  was pressed in. 
     Example 6 
     Using the test container A, a 50% position of the portion  214 B on the first flow path  231  and a 50% position of the portion  214 A on the second accommodation portion  222  were pressed by a ball plunger to carry out liquid feeding (see  FIG. 7 ). In this case, the portions  214 A and  214 B were pressed at the same time. 
     Example 7 
     Using the test container B, a 30% position of the portion  214 A on the second accommodation portion  222  was pressed by a ball plunger to carry out liquid feeding (see  FIG. 5 ). 
     Example 8 
     Using the test container C, a 30% position of the portion  214 A on the second accommodation portion  222  was pressed by a ball plunger to carry out liquid feeding (see  FIG. 5 ). 
     Example 9 
     Using the test container D, a 30% position of the portion  214 A on the second accommodation portion  222  was pressed by a ball plunger to carry out liquid feeding (see  FIG. 5 ). 
     Comparative Example 1 
     Using the test container A, a 50% position of the portion  214 A on the second accommodation portion  222  was pressed by a ball plunger to carry out liquid feeding. 
     Comparative Example 2 
     Using the test container A, a 75% position of the portion  214 A on the second accommodation portion  222  was pressed by a ball plunger to carry out liquid feeding. 
     Table 1 collectively shows the container, the liquid feeding method, and the evaluation result of each example. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 1 
               
             
            
               
                   
                   
               
               
                   
                 Liquid feeding method 
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Second accommodation 
                 Evaluation 
               
               
                   
                   
                 First flow path 
                 portion 
                 Liquid feeding 
               
               
                   
                 Pressing method 
                 Pressing position 
                 Pressing position 
                 properties 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Example 1 
                 Test container A 
                 Only second accommodation portion 
                   
                 30% position 
                 E 
               
               
                 Example 2 
                 Test container A 
                 Only second accommodation portion 
                   
                 15% position 
                 D 
               
               
                 Example 3 
                 Test container A 
                 Pressing first flow path and second 
                 25% position 
                 50% position 
                 B 
               
               
                   
                   
                 accommodation portion at the same 
               
               
                   
                   
                 time 
               
               
                 Example 4 
                 Test container A 
                 Pressing first flow path and second 
                 50% position 
                 50% position 
                 A 
               
               
                   
                   
                 accommodation portion at the same 
               
               
                   
                   
                 time 
               
               
                 Example 5 
                 Test container A 
                 Pressing first flow path and second 
                 75% position 
                 50% position 
                 A 
               
               
                   
                   
                 accommodation portion at the same 
               
               
                   
                   
                 time 
               
               
                 Example 6 
                 Test container A 
                 Pressing first flow path and second 
                 25% position 
                 50% position 
                 A 
               
               
                   
                   
                 accommodation portion in this order 
               
               
                 Example 7 
                 Test container B 
                 Only second accommodation portion 
                   
                 30% position 
                 E 
               
               
                 Example 8 
                 Test container C 
                 Only second accommodation portion 
                   
                 30% position 
                 E 
               
               
                 Example 9 
                 Test container D 
                 Only second accommodation portion 
                   
                 30% position 
                 E 
               
               
                 Comparative 
                 Test container A 
                 Only second accommodation portion 
                   
                 50% position 
                 F 
               
               
                 Example 1 
               
               
                 Comparative 
                 Test container A 
                 Only second accommodation portion 
                   
                 75% position 
                 F 
               
               
                 Example 2 
               
               
                   
               
            
           
         
       
     
     As shown in Table 1, as shown in Examples 1 and 2, by pressing the position of the second accommodation portion  222  closer to the first flow path  231 , it was possible to improve the liquid feeding properties to the third accommodation portion  223  side, compared to Comparative Examples 1 and 2 in which the central portion of the second accommodation portion  222  (50% position) and the position closer to the second flow path  232  (75% position) were pressed. 
     In Examples 1 and 7 to 9 in which, while the main body portions of the test containers have different formation materials, but have the same shape, and the same position is pressed, the same results were obtained. It is considered that the liquid feeding properties of the technology of the present disclosure do not depend on the material of the main body portion, and the same results can be obtained. 
     As in Examples 1 and 2, in a case where the liquid is fed with a configuration in which the position of the portion  214 A forming the upper wall surface of the second accommodation portion  222  closer to the first flow path  231  is pressed by using one plunger (configuration of liquid feeding device of the first embodiment), the liquid feeding properties were higher in a case where the pressing position is closer to the first flow path. 
     As in Examples 3 to 6, in a case where the liquid is fed with a configuration in which the two portions of the first portion  214 B forming the upper wall surface of the first flow path  231  and the second portion  214 A forming the upper wall surface of the second accommodation portion  222  are pressed (configuration of liquid feeding device of the second embodiment) by using the two plungers, the liquid feeding properties were higher than in Examples 1 and 2, and more excellent effect of preventing the backflow to the first flow path  231  was obtained. It was found that it is particularly preferable that the pressing position of the first flow path  231  includes the center position (50% position) in the flow path length direction on the second accommodation portion  222  side, in order to improve the liquid feeding properties. 
     EXPLANATION OF REFERENCES 
     
         
         
           
               1 ,  1 A,  2 ,  3 ,  3 A,  4 ,  5 ,  6 ,  211 : Test container 
               10 ,  10 A,  10 B,  10 C,  10 D,  10 E,  10 F,  10 G: Container main body 
               12 ,  12 A,  12 B,  12 C,  12 D,  12 E,  12 F,  12 G: Main body portion 
               14 : Upper lid member 
               14 A: Portion (first part) 
               14 B: Second portion 
               21 : First accommodation portion 
               21   b : Upper wall surface of first accommodation portion 
               22 : Second accommodation portion 
               22   a : Inner bottom surface of second accommodation portion 
               22   b : upper wall surface of second accommodation portion 
               22   c : Inner side surface of second accommodation portion 
               23 : Third accommodation portion 
               31 : First flow path 
               31   a : Inner bottom surface of first flow path 
               32 : Second flow path 
               32   a : Inner bottom surface of second flow path 
               33 ,  43 : Corner 
               34 : Hydrophobic surface 
               40 : Stepped portion 
               41 ,  42 : Step 
               50 ,  55 : Pressing machine 
               51 ,  52 : Plunger 
               53 ,  54 : Cylinder 
               100 : nucleic acid extraction test device 
               101 : test container 
               102 : Transportation unit 
               106 : Dispenser 
               107 : Magnetic field generation and movement unit 
               108 : Temperature control unit 
               110 : Container main body 
               112 : Main body portion 
               114 : Upper lid member 
               120 : Magnetism collecting chamber (accommodation portion) 
               121 : Cleaning chamber (first accommodation portion) 
               122 : PCR chamber (second accommodation portion) 
               122   a : Inner bottom surface of PCR chamber 
               123 : Detection chamber (third accommodation portion) 
               125 : Chromatographic carrier accommodation portion 
               128 : Chromatographic carrier 
               130 ,  131 ,  132 ,  135 ,  145 : flow path 
               150 : specimen solution 
               151 : Cleaning solution 
               152 : PCR solution 
               153 : Development solution 
               160 - 163 : Syringe 
               170 : Movement mechanism 
               201 : Liquid feeding device 
               210 : Container main body 
               211 : Test container 
               212 : Main body portion 
               214 : Upper lid member 
               221 : First accommodation portion 
               222 : Second accommodation portion 
               223 : Third accommodation portion 
               231 : First flow path 
               232 : Second flow path 
               240 : Stepped portion 
               241 ,  242 ,  243 : step 
             M: Magnet 
             P: Magnetic particles