Patent Publication Number: US-11643675-B2

Title: Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides

Description:
RELATED APPLICATIONS 
     This application is a continuation of U.S. patent application Ser. No. 15/307,914 filed Oct. 31, 2016, now U.S. Pat. No. 11,046,984 issued on Jun. 29, 2021, which is a national stage application, filed under 35 U.S.C. § 371, of PCT International Patent Application No. PCT/US2015/030823, filed on May 14, 2015, and claims benefit of priority to U.S. Provisional Patent Application No. 61/993,742, filed on May 15, 2014, both of which, including their contents, are incorporated herein by reference in their entireties. 
    
    
     SEQUENCE LISTING 
     The instant application contains a Sequence Listing which has been submitted via EFS-Web. The content of the text file named “37847-517001US_ST25.txt”, which was created on Oct. 20, 2017 and is 791 KB in size, is hereby incorporated by reference in its entirety. 
     FIELD OF THE INVENTION 
     The invention provides compositions and methods for producing purified oligosaccharides, in particular certain fucosylated oligosaccharides that are typically found in human milk. 
     BACKGROUND OF THE INVENTION 
     Human milk contains a diverse and abundant set of neutral and acidic oligosaccharides. More than 130 different complex oligosaccharides have been identified in human milk, and their structural diversity and abundance is unique to humans. Although these molecules may not be utilized directly by infants for nutrition, they nevertheless serve critical roles in the establishment of a healthy gut microbiome, in the prevention of disease, and in immune function. Prior to the invention described herein, the ability to produce human milk oligosaccharides (HMOS) inexpensively was problematic. For example, their production through chemical synthesis was limited by stereo-specificity issues, precursor availability, product impurities, and high overall cost. As such, there is a pressing need for new strategies to inexpensively manufacture large quantities of HMOS. 
     SUMMARY OF THE INVENTION 
     The invention features an efficient and economical method for producing fucosylated oligosaccharides. Such production of a fucosylated oligosaccharide is accomplished using an isolated nucleic acid comprising a sequence encoding a lactose-utilizing α (1,2) fucosyltransferase gene product (e.g., polypeptide or protein), which is operably linked to one or more heterologous control sequences that direct the production of the recombinant fucosyltransferase gene product in a host production bacterium such as  Escherichia coli  ( E. coli ). 
     The present disclosure provides novel α (1,2) fucosyltransferases (also referred to herein as α(1,2) FTs) that utilize lactose and catalyzes the transfer of an L-fucose sugar from a GDP-fucose donor substrate to an acceptor substrate in an alpha-1,2-linkage. In a preferred embodiment, the acceptor substrate is an oligosaccharide. The α(1,2) fucosyltransferases identified and described herein are useful for expressing in host bacterium for the production of human milk oligosaccharides (HMOS), such as fucosylated oligosaccharides. Exemplary fucosylated oligosaccharides produced by the methods described herein include 2′-fucosyllactose (2′FL), lactodifucotetraose (LDFT), lacto-N-fucopentaose I (LNF I), or lacto-N-difucohexaose I (LDFH I). The “α(1,2) fucosyltransferases” disclosed herein encompasses the amino acid sequences of the α(1,2) fucosyltransferases and the nucleic acid sequences that encode the α(1,2) fucosyltransferases, as well as variants and fragments thereof that exhibit α(1,2) fucosyltransferase activity. Also within the invention is a nucleic acid construct comprising an isolated nucleic acid encoding a lactose-accepting α (1,2) fucosyltransferase enzyme, said nucleic acid being optionally operably linked to one or more heterologous control sequences that direct the production of the enzyme in a host bacteria production strain. 
     The amino acid sequence of the lactose-accepting α(1,2) fucosyltransferases described herein is at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% identity to  Helicobacter pylori  26695 alpha-(1,2) fucosyltransferase (futC or SEQ ID NO: 1). Preferably, the lactose-accepting α(1,2) fucosyltransferases described herein is at least 22% identical to  H. pylori  FutC, or SEQ ID NO: 1. 
     In another aspect, the amino acid sequence of the lactose-accepting α(1,2) fucosyltransferases described herein is at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% identity to  Bacteroides vulgatus  alpha-(1,2) fucosyltransferase (FutN or SEQ ID NO: 3). Preferably, the lactose-accepting α(1,2) fucosyltransferases described herein is at least 25% identical to  B. vlugatos  FutN, or SEQ ID NO: 3. 
     Alternatively, the exogenous α (1,2) fucosyltransferase preferably comprises at least at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% identity to any one of the novel α (1,2) fucosyltransferases disclosed herein, for example, to the amino acid sequences in Table 1. 
     Exemplary α(1,2) fucosyltransferases include, but are not limited to,  Prevotella melaninogenica  FutO,  Clostridium bolteae  FutP,  Clostridium bolteae+ 13 FutP,  Lachnospiraceae  sp. FutQ,  Methanosphaerula palustris  FutR,  Tannerella  sp. FutS,  Bacteroides caccae  FutU,  Butyrivibrio  FutV,  Prevotella  sp. FutW,  Parabacteroides johnsonii  FutX,  Akkermansia muciniphilia  FutY,  Salmonella enterica  FutZ,  Bacteroides  sp. FutZA. For example, the α(1,2) fucosyltransferases comprise the amino acid sequences comprising any one of the following:  Prevotella melaninogenica  FutO (SEQ ID NO: 10),  Clostridium bolteae  FutP (SEQ ID NO: 11),  Clostridium bolteae+ 13 FutP (SEQ ID NO: 292),  Lachnospiraceae  sp. FutQ (SEQ ID NO: 12),  Methanosphaerula palustris  FutR (SEQ ID NO: 13),  Tannerella  sp. FutS (SEQ ID NO: 14),  Bacteroides  caccae FutU (SEQ ID NO: 15),  Butyrivibrio  FutV (SEQ ID NO: 16),  Prevotella  sp. FutW (SEQ ID NO: 17), Parabacteroides  johnsonii  FutX (SEQ ID NO: 18),  Akkermansia muciniphilia  FutY (SEQ ID NO: 19),  Salmonella enterica  FutZ (SEQ ID NO: 20), and  Bacteroides  sp. FutZA (SEQ ID NO: 21), or a functional variant or fragment thereof. Other exemplary α(1,2) fucosyltransferases include any of the enzymes listed in Table 1, or functional variants or fragments thereof. 
     The present invention features a method for producing a fucosylated oligosaccharide in a bacterium by providing bacterium that express at least one exogenous lactose-utilizing α(1,2) fucosyltransferase. The amino acid sequence of the exogenous lactose-utilizing α(1,2) fucosyltransferase is preferably at least 22% identical to  H. pylori  FutC or at least 25% identical to  B. vulgatus  FutN. In one aspect, the bacterium also expresses one or more exogenous lactose-utilizing α(1,3) fucosyltransferase enzymes and/or one or more exogenous lactose-utilizing α(1,4) fucosyltransferase enzymes. The combination of fucosyltransferases expressed in the production bacterium is dependent upon the desired fucosylated oligosaccharide product. The method disclosed herein further includes retrieving the fucosylated oligosaccharide from said bacterium or from a culture supernatant of said bacterium. 
     Examples of suitable α(1,3) fucosyltransferase enzymes include, but are not limited to  Helicobacter pylori  26695 futA gene (GenBank Accession Number HV532291 (GI:365791177), incorporated herein by reference),  H. hepaticus  Hh0072,  H. pylori  11639 FucT, and  H. pylori  UA948 FucTa (e.g., GenBank Accession Number AF194963 (GI:28436396), incorporated herein by reference) (Rasko, D. A., Wang, G., Palcic, M. M. &amp; Taylor, D. E. J Biol Chem 275, 4988-4994 (2000)). Examples of suitable α(1,4) fucosyltransferase enzymes include, but are not limited to  H. pylori  UA948 FucTa (which has has relaxed acceptor specificity and is able to generate both α(1,3)- and α(1,4)-fucosyl linkages). An example of an enzyme possessing only α(1,4) fucosyltransferase activity is given by the FucT III enzyme from  Helicobacter pylori  strain DMS6709 (e.g., GenBank Accession Number AY450598.1 (GI:40646733), incorporated herein by reference) (S. Rabbani, V. Miksa, B. Wipf, B. Ernst,  Glycobiology  15, 1076-83 (2005).) 
     The invention also features a nucleic acid construct or a vector comprising a nucleic acid enconding at least one α (1,2) fucosyltransferase or variant, or fragment thereof, as described herein. The vector can further include one or more regulatory elements, e.g., a heterologous promoter. By “heterologous” is meant that the control sequence and protein-encoding sequence originate from different bacterial strains. The regulatory elements can be operably linked to a gene encoding a protein, a gene construct encoding a fusion protein gene, or a series of genes linked in an operon in order to express the fusion protein. In yet another aspect, the invention comprises an isolated recombinant cell, e.g., a bacterial cell containing an aforementioned nucleic acid molecule or vector. The nucleic acid is optionally integrated into the genome of the host bacterium. In some embodiments, the nucleic acid construct also further comprises one or more α(1,3) fucosyltransferases and/or α(1,4) fucosyltransferases. Alternatively, the α (1,2) fucosyltransferase also exhibits α(1,3) fucosyltransferase and/or α(1,4) fucosyltransferase activity. 
     The bacterium utilized in the production methods described herein is genetically engineered to increase the efficiency and yield of fucosylated oligosaccharide products. For example, the host production bacterium is characterized as having a reduced level of β-galactosidase activity, a defective colanic acid synthesis pathway, an inactivated ATP-dependent intracellular protease, an inactivated lacA, or a combination thereof. In one embodiment, the bacterium is characterized as having a reduced level of β-galactosidase activity, a defective colanic acid synthesis pathway, an inactivated ATP-dependent intracellular protease, and an inactivated lacA. 
     As used herein, an “inactivated” or “inactivation of a” gene, encoded gene product (i.e., polypeptide), or pathway refers to reducing or eliminating the expression (i.e., transcription or translation), protein level (i.e., translation, rate of degradation), or enzymatic activity of the gene, gene product, or pathway. In the instance where a pathway is inactivated, preferably one enzyme or polypeptide in the pathway exhibits reduced or negligible activity. For example, the enzyme in the pathway is altered, deleted or mutated such that the product of the pathway is produced at low levels compared to a wild-type bacterium or an intact pathway. Alternatively, the product of the pathway is not produced. Inactivation of a gene is achieved by deletion or mutation of the gene or regulatory elements of the gene such that the gene is no longer transcribed or translated. Inactivation of a polypeptide can be achieved by deletion or mutation of the gene that encodes the gene product or mutation of the polypeptide to disrupt its activity. Inactivating mutations include additions, deletions or substitutions of one or more nucleotides or amino acids of a nucleic acid or amino acid sequence that results in the reduction or elimination of the expression or activity of the gene or polypeptide. In other embodiments, inactivation of a polypeptide is achieved through the addition of exogenous sequences (i.e., tags) to the N or C-terminus of the polypeptide such that the activity of the polypeptide is reduced or eliminated (i.e., by steric hindrance). 
     A host bacterium suitable for the production systems described herein exhibits an enhanced or increased cytoplasmic or intracellular pool of lactose and/or GDP-fucose. For example, the bacterium is  E. coli  and endogenous  E. coli  metabolic pathways and genes are manipulated in ways that result in the generation of increased cytoplasmic concentrations of lactose and/or GDP-fucose, as compared to levels found in wild type  E. coli . Preferably, the bacterium accumulates an increased intracellular lactose pool and an increased intracellular GDP-fucose pool. For example, the bacteria contain at least 10%, 20%, 50%, or 2×, 5×, 10× or more of the levels of intracellular lactose and/or intracellular GDP-fucose compared to a corresponding wild type bacteria that lacks the genetic modifications described herein. 
     Increased intracellular concentration of lactose in the host bacterium compared to wild-type bacterium is achieved by manipulation of genes and pathways involved in lactose import, export and catabolism. In particular, described herein are methods of increasing intracellular lactose levels in  E. coli  genetically engineered to produce a human milk oligosaccharide by simultaneous deletion of the endogenous β-galactosidase gene (lacZ) and the lactose operon repressor gene (lad). During construction of this deletion, the lacIq promoter is placed immediately upstream of (contiguous with) the lactose permease gene, lacY, i.e., the sequence of the lacIq promoter is directly upstream and adjacent to the start of the sequence encoding the lacY gene, such that the lacY gene is under transcriptional regulation by the lacIq promoter. The modified strain maintains its ability to transport lactose from the culture medium (via LacY), but is deleted for the wild-type chromosomal copy of the lacZ (encoding β-galactosidase) gene responsible for lactose catabolism. Thus, an intracellular lactose pool is created when the modified strain is cultured in the presence of exogenous lactose. 
     Another method for increasing the intracellular concentration of lactose in  E. coli  involves inactivation of the lacA gene. A inactivating mutation, null mutation, or deletion of lacA prevents the formation of intracellular acetyl-lactose, which not only removes this molecule as a contaminant from subsequent purifications, but also eliminates  E. coli &#39;s ability to export excess lactose from its cytoplasm (Danchin A. Cells need safety valves. Bioessays 2009, July; 31(7):769-73.), thus greatly facilitating purposeful manipulations of the  E. coli  intracellular lactose pool. 
     The invention also provides methods for increasing intracellular levels of GDP-fucose in a bacterium by manipulating the organism&#39;s endogenous colanic acid biosynthesis pathway. This increase is achieved through a number of genetic modifications of endogenous  E. coli  genes involved either directly in colanic acid precursor biosynthesis, or in overall control of the colanic acid synthetic regulon. Particularly preferred is inactivation of the genes or encoded polypeptides that act in the colanic acid synthesis pathway after the production of GDP-fucose (the donor substrate) and before the generation of colanic acid. Exemplary colanic acid synthesis genes include, but are not limited to: a wcaJ gene, (e.g., GenBank Accession Number (amino acid) BAA15900 (GI:1736749), incorporated herein by reference), a wcaA gene (e.g., GenBank Accession Number (amino acid) BAA15912.1 (GI:1736762), incorporated herein by reference), a wcaC gene (e.g., GenBank Accession Number (amino acid) BAE76574.1 (GI:85675203), incorporated herein by reference), a wcaE gene (e.g., GenBank Accession Number (amino acid) BAE76572.1 (GI:85675201), incorporated herein by reference), a weal gene (e.g., GenBank Accession Number (amino acid) BAA15906.1 (GI:1736756), incorporated herein by reference), a wcaL gene (e.g., GenBank Accession Number (amino acid) BAA15898.1 (GI:1736747), incorporated herein by reference), a wcaB gene (e.g., GenBank Accession Number (amino acid) BAA15911.1 (GI:1736761), incorporated herein by reference), a wcaF gene (e.g., GenBank Accession Number (amino acid) BAA15910.1 (GI:1736760), incorporated herein by reference), a wzxE gene (e.g., GenBank Accession Number (amino acid) BAE77506.1 (GI:85676256), incorporated herein by reference), a wzxC gene, (e.g., GenBank Accession Number (amino acid) BAA15899 (GI:1736748), incorporated herein by reference), a wcaD gene, (e.g., GenBank Accession Number (amino acid) BAE76573 (GI:85675202), incorporated herein by reference), a wza gene (e.g., GenBank Accession Number (amino acid) BAE76576 (GI:85675205), incorporated herein by reference), a wzb gene (e.g., GenBank Accession Number (amino acid) BAE76575 (GI:85675204), incorporated herein by reference), and a wzc gene (e.g., GenBank Accession Number (amino acid) BAA15913 (GI:1736763), incorporated herein by reference). 
     Preferably, a host bacterium, such as  E. coli , is genetically engineered to produce a human milk oligosaccharide by the inactivation of the wcaJ gene, which encoding the UDP-glucose lipid carrier transferase. The inactivation of the wcaJ gene can be by deletion of the gene, a null mutation, or inactivating mutation of the wcaJ gene, such that the activity of the encoded wcaJ is reduced or eliminated compared to wild-type  E. coli . In a wcaJ null background, GDP-fucose accumulates in the  E. coli  cytoplasm. 
     Over-expression of a positive regulator protein, RcsA (e.g., GenBank Accession Number M58003 (GI:1103316), incorporated herein by reference), in the colanic acid synthesis pathway results in an increase in intracellular GDP-fucose levels. Over-expression of an additional positive regulator of colanic acid biosynthesis, namely RcsB (e.g., GenBank Accession Number E04821 (GI:2173017), incorporated herein by reference), is also utilized, either instead of or in addition to over-expression of RcsA, to increase intracellular GDP-fucose levels. 
     Alternatively, colanic acid biosynthesis is increased following the introduction of a mutation into the  E. coli  lon gene (e.g., GenBank Accession Number L20572 (GI:304907), incorporated herein by reference). Lon is an adenosine-5′-triphosphate (ATP)-dependant intracellular protease that is responsible for degrading RcsA, mentioned above as a positive transcriptional regulator of colanic acid biosynthesis in  E. coli . In a lon null background, RcsA is stabilized, RcsA levels increase, the genes responsible for GDP-fucose synthesis in  E. coli  are up-regulated, and intracellular GDP-fucose concentrations are enhanced. Mutations in lon suitable for use with the methods presented herein include null mutations or insertions that disrupt the expression or function of ion. 
     A functional lactose permease gene is also present in the bacterium. The lactose permease gene is an endogenous lactose permease gene or an exogenous lactose permease gene. For example, the lactose permease gene comprises an  E. coli  lacY gene (e.g., GenBank Accession Number V00295 (GI:41897), incorporated herein by reference). Many bacteria possess the inherent ability to transport lactose from the growth medium into the cell, by utilizing a transport protein that is either a homolog of the  E. coli  lactose permease (e.g., as found in  Bacillus licheniformis ), or a transporter that is a member of the ubiquitous PTS sugar transport family (e.g., as found in  Lactobacillus casei  and  Lactobacillus rhamnosus ). For bacteria lacking an inherent ability to transport extracellular lactose into the cell cytoplasm, this ability is conferred by an exogenous lactose transporter gene (e.g.,  E. coli  lacY) provided on recombinant DNA constructs, and supplied either on a plasmid expression vector or as exogenous genes integrated into the host chromosome. 
     As described herein, in some embodiments, the host bacterium preferably has a reduced level of β-galactosidase activity. In the embodiment in which the bacterium is characterized by the deletion of the endogenous β-galactosidase gene, an exogenous β-galactosidase gene is introduced to the bacterium. For example, a plasmid expressing an exogenous β-galactosidase gene is introduced to the bacterium, or recombined or integrated into the host genome. For example, the exogenous β-galactosidase gene is inserted into a gene that is inactivated in the host bacterium, such as the lon gene. 
     The exogenous b-galactosidase gene is a functional b-galactosidase gene characterized by a reduced or low level of b-galactosidase activity compared to β-galactosidase activity in wild-type bacteria lacking any genetic manipulation. Exemplary β-galactosidase genes include  E. coli  lacZ and β-galactosidase genes from any of a number of other organisms (e.g., the lac4 gene of  Kluyveromyces lactis  (e.g., GenBank Accession Number M84410 (GI:173304), incorporated herein by reference) that catalyzes the hydrolysis of b-galactosides into monosaccharides. The level of β-galactosidase activity in wild-type  E. coli  bacteria is, for example, 6,000 units. Thus, the reduced β-galactosidase activity level encompassed by engineered host bacterium of the present invention includes less than 6,000 units, less than 5,000 units, less than 4,000 units, less than 3,000 units, less than 2,000 units, less than 1,000 units, less than 900 units, less than 800 units, less than 700 units, less than 600 units, less than 500 units, less than 400 units, less than 300 units, less than 200 units, less than 100 units, or less than 50 units. Low, functional levels of β-galactosidase include β-galactosidase activity levels of between 0.05 and 1,000 units, e.g., between 0.05 and 750 units, between 0.05 and 500 units, between 0.05 and 400 units, between 0.05 and 300 units, between 0.05 and 200 units, between 0.05 and 100 units, between 0.05 and 50 units, between 0.05 and 10 units, between 0.05 and 5 units, between 0.05 and 4 units, between 0.05 and 3 units, or between 0.05 and 2 units of β-galactosidase activity. For unit definition and assays for determining β-galactosidase activity, see Miller J H, Laboratory CSH. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor, N.Y.; 1972; (incorporated herein by reference). This low level of cytoplasmic β-galactosidase activity is not high enough to significantly diminish the intracellular lactose pool. The low level of β-galactosidase activity is very useful for the facile removal of undesired residual lactose at the end of fermentations. 
     Optionally, the bacterium has an inactivated thyA gene. Preferably, a mutation in a thyA gene in the host bacterium allows for the maintenance of plasmids that carry thyA as a selectable marker gene. Exemplary alternative selectable markers include antibiotic resistance genes such as BLA (beta-lactamase), or proBA genes (to complement a proAB host strain proline auxotropy) or purA (to complement a purA host strain adenine auxotrophy). 
     In one aspect, the  E. coli  bacterium comprises the genotype ΔampC::P trp   B cI, Δ(lacI-lacZ)::FRT, P lacIq lacY + , ΔwcaJ::FRT, thyA::Tn10, Δlon:(npt3, lacZ + ), ΔlacA, and also comprises any one of the exogenous α(1,2) fucosyltransferases described herein. 
     The bacterium comprising these characteristics is cultured in the presence of lactose. In some cases, the method further comprises culturing the bacterium in the presence of tryptophan and in the absence of thymidine. The fucosylated oligosaccharide is retrieved from the bacterium (i.e., a cell lysate) or from a culture supernatant of the bacterium. 
     The invention provides a purified fucosylated oligosaccharide produced by the methods described herein. The fucosylated oligosaccharide is purified for use in therapeutic or nutritional products, or the bacterium is used directly in such products. The fucosylated oligosaccharide produced by the engineered bacterium is 2′-fucosyllactose (2′-FL) or lactodifucotetraose (LDFT). The new alpha 1,2-fucosyltransferases are also useful to synthesize HMOS of larger molecular weight bearing alpha 1,2 fucose moieties, e.g., lacto-N-fucopentaose (LNF I) and lacto-N-difucohexaose (LDFH I). For example, to produce LDFT, the host bacterium is engineered to express an exogenous α (1,2) fucosyltransferase that also possesses α (1,3) fucosyltransferase activity, or an exogenous α (1,2) fucosyltransferase and an exogenous α (1,3) fucosyltransferase. For the production of LNF I and LDFH I, the host bacterium is engineered to express an exogenous α (1,2) fucosyltransferase that also possesses α (1,3) fucosyltransferase activity and/or α (1,4) fucosyltransferase activity, or an exogenous α (1,2) fucosyltransferase, an exogenous α (1,3) fucosyltransferas, and an exogenous α (1,4) fucosyltransferase. 
     A purified fucosylated oligosaccharide produced by the methods described above is also within the invention. The purified oligosaccharide (2′-FL) obtained at the end of the process is a white/slightly off-white, crystalline, sweet powder. For example, an engineered bacterium, bacterial culture supernatant, or bacterial cell lysate according to the invention comprises 2′-FL, LDFT, LNF I or LDFH I produced by the methods described herein, and does not substantially comprise a other fucosylated oligosaccharides prior to purification of the fucosylated oligosaccharide products from the cell, culture supernatant, or lysate. As a general matter, the fucosylated oligosaccharide produced by the methods contains a negligible amount of 3-FL in a 2′-FL-containing cell, cell lysate or culture, or supernatant, e.g., less than 1% of the level of 2′-FL or 0.5% of the level of 2′-FL. Moreover, the fucosylated oligosaccharide produced by the methods described herein also have a minimal amount of contaminating lactose, which can often be co-purified with the fucosylated oligosaccharide product, such as 2′FL. This reduction in contaminating lactose results from the reduced level of β-galactosidase activity present in the engineered host bacterium. 
     A purified oligosaccharide, e.g., 2′-FL, LDFT, LNF I, or LDFH I, is one that is at least 90%, 95%, 98%, 99%, or 100% (w/w) of the desired oligosaccharide by weight. Purity is assessed by any known method, e.g., thin layer chromatography or other chromatographic techniques known in the art. The invention includes a method of purifying a fucosylated oligosaccharide produced by the genetically engineered bacterium described above, which method comprises separating the desired fucosylated oligosaccharide (e.g., 2′-FL) from contaminants in a bacterial cell lysate or bacterial cell culture supernatant of the bacterium. 
     The oligosaccharides are purified and used in a number of products for consumption by humans as well as animals, such as companion animals (dogs, cats) as well as livestock (bovine, equine, ovine, caprine, or porcine animals, as well as poultry). For example, a pharmaceutical composition comprises purified 2′-FL and a pharmaceutically-acceptable excipient that is suitable for oral administration. Large quantities of 2′-FL are produced in bacterial hosts, e.g., an  E. coli  bacterium comprising an exogenous α (1,2) fucosyltransferase gene. 
     A method of producing a pharmaceutical composition comprising a purified human milk oligosaccharide (HMOS) is carried out by culturing the bacterium described above, purifying the HMOS produced by the bacterium, and combining the HMOS with an excipient or carrier to yield a dietary supplement for oral administration. These compositions are useful in methods of preventing or treating enteric and/or respiratory diseases in infants and adults. Accordingly, the compositions are administered to a subject suffering from or at risk of developing such a disease. 
     The invention also provides methods of identifying an α (1,2) fucosyltransferase gene capable of synthesizing fucosylated oligosaccharides in a host bacterium, i.e., 2′-fucosyllactose (2′-FL) in  E. coli . The method of identifying novel lactose-utilizing, α(1,2)fucosyltransferase enzyme comprises the following steps: 
     1) performing a computational search of sequence databases to define a broad group of simple sequence homologs of any known, lactose-utilizing α(1,2)fucosyltransferase; 
     2) using the list from step (1), deriving a search profile containing common sequence and/or structural motifs shared by the members of the list; 
     3) searching sequence databases, using a derived search profile based on the common sequence or structural motif from step (2) as query, and identifying a candidate sequences, wherein a sequence homology to a reference lactose-utilizing α(1,2)fucosyltransferase is a predetermined percentage threshold; 
     4) compiling a list of candidate organisms, said organisms being characterized as expressing α(1,2)fucosyl-glycans in a naturally-occurring state; 
     5) selecting candidate sequences that are derived from candidate organisms to generate a list of candidate lactose-utilizing enzymes; 
     6) expressing the candidate lactose-utilizing enzyme in a host organism; and 
     7) testing for lactose-utilizing α(1,2)fucosyltransferase activity, wherein detection of the desired fucosylated oligosaccharide product in said organism indicates that the candidate sequence comprises a novel lactose-utilizing α(1,2)fucosyltransferase. In another embodiment, the search profile is generated from a multiple sequence alignment of the amino acid sequences of more than one enzyme with known α(1,2)fucosyltransferase activity. The database search can then be designed to refine and iteratively search for novel α(1,2)fucosyltransferases with significant sequence similarity to the multiple sequence alignment query. 
     The invention provides a method of treating, preventing, or reducing the risk of infection in a subject comprising administering to said subject a composition comprising a purified recombinant human milk oligosaccharide, wherein the HMOS binds to a pathogen and wherein the subject is infected with or at risk of infection with the pathogen. In one aspect, the infection is caused by a Norwalk-like virus or  Campylobacter jejuni . The subject is preferably a mammal in need of such treatment. The mammal is, e.g., any mammal, e.g., a human, a primate, a mouse, a rat, a dog, a cat, a cow, a horse, or a pig. In a preferred embodiment, the mammal is a human. For example, the compositions are formulated into animal feed (e.g., pellets, kibble, mash) or animal food supplements for companion animals, e.g., dogs or cats, as well as livestock or animals grown for food consumption, e.g., cattle, sheep, pigs, chickens, and goats. Preferably, the purified HMOS is formulated into a powder (e.g., infant formula powder or adult nutritional supplement powder, each of which is mixed with a liquid such as water or juice prior to consumption) or in the form of tablets, capsules or pastes or is incorporated as a component in dairy products such as milk, cream, cheese, yogurt or kefir, or as a component in any beverage, or combined in a preparation containing live microbial cultures intended to serve as probiotics, or in prebiotic preparations to enhance the growth of beneficial microorganisms either in vitro or in vivo. 
     Polynucleotides, polypeptides, and oligosaccharides of the invention are purified and/or isolated. Purified defines a degree of sterility that is safe for administration to a human subject, e.g., lacking infectious or toxic agents. Specifically, as used herein, an “isolated” or “purified” nucleic acid molecule, polynucleotide, polypeptide, protein or oligosaccharide, is substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. For example, purified HMOS compositions are at least 60% by weight (dry weight) the compound of interest. Preferably, the preparation is at least 75%, more preferably at least 90%, and most preferably at least 99%, by weight the compound of interest. Purity is measured by any appropriate standard method, for example, by column chromatography, thin layer chromatography, or high-performance liquid chromatography (HPLC) analysis. For example, a “purified protein” refers to a protein that has been separated from other proteins, lipids, and nucleic acids with which it is naturally associated. Preferably, the protein constitutes at least 10, 20, 50, 70, 80, 90, 95, 99-100% by dry weight of the purified preparation. 
     Similarly, by “substantially pure” is meant an oligosaccharide that has been separated from the components that naturally accompany it. Typically, the oligosaccharide is substantially pure when it is at least 60%, 70%, 80%, 90%, 95%, or even 99%, by weight, free from the proteins and naturally-occurring organic molecules with which it is naturally associated. 
     By “isolated nucleic acid” is meant a nucleic acid that is free of the genes which, in the naturally-occurring genome of the organism from which the DNA of the invention is derived, flank the gene. The term covers, for example: (a) a DNA which is part of a naturally occurring genomic DNA molecule, but is not flanked by both of the nucleic acid sequences that flank that part of the molecule in the genome of the organism in which it naturally occurs; (b) a nucleic acid incorporated into a vector or into the genomic DNA of a prokaryote or eukaryote in a manner, such that the resulting molecule is not identical to any naturally occurring vector or genomic DNA; (c) a separate molecule such as a cDNA, a genomic fragment, a fragment produced by polymerase chain reaction (PCR), or a restriction fragment; and (d) a recombinant nucleotide sequence that is part of a hybrid gene, i.e., a gene encoding a fusion protein. Isolated nucleic acid molecules according to the present invention further include molecules produced synthetically, as well as any nucleic acids that have been altered chemically and/or that have modified backbones. 
     A “heterologous promoter” is a promoter which is different from the promoter to which a gene or nucleic acid sequence is operably linked in nature. 
     The term “overexpress” or “overexpression” refers to a situation in which more factor is expressed by a genetically-altered cell than would be, under the same conditions, by a wild type cell. Similarly, if an unaltered cell does not express a factor that it is genetically altered to produce, the term “express” (as distinguished from “overexpress”) is used indicating the wild type cell did not express the factor at all prior to genetic manipulation. 
     The terms “treating” and “treatment” as used herein refer to the administration of an agent or formulation to a clinically symptomatic individual afflicted with an adverse condition, disorder, or disease, so as to effect a reduction in severity and/or frequency of symptoms, eliminate the symptoms and/or their underlying cause, and/or facilitate improvement or remediation of damage. The terms “preventing” and “prevention” refer to the administration of an agent or composition to a clinically asymptomatic individual who is susceptible to a particular adverse condition, disorder, or disease, and thus relates to the prevention of the occurrence of symptoms and/or their underlying cause. 
     By the terms “effective amount” and “therapeutically effective amount” of a formulation or formulation component is meant a nontoxic but sufficient amount of the formulation or component to provide the desired effect. 
     The transitional term “comprising,” which is synonymous with “including,” “containing,” or “characterized by,” is inclusive or open-ended and does not exclude additional, unrecited elements or method steps. By contrast, the transitional phrase “consisting of” excludes any element, step, or ingredient not specified in the claim. The transitional phrase “consisting essentially of” limits the scope of a claim to the specified materials or steps “and those that do not materially affect the basic and novel characteristic(s)” of the claimed invention. 
     The host organism used to express the lactose-accepting fucosyltransferase gene is typically the enterobacterium  Escherichia coli  K12 ( E. coli ).  E. coli  K-12 is not considered a human or animal pathogen nor is it toxicogenic.  E. coli  K-12 is a standard production strain of bacteria and is noted for its safety due to its poor ability to colonize the colon and establish infections (see, e.g., epa.gov/oppt/biotech/pubs/fra/fra004.htm). However, a variety of bacterial species may be used in the oligosaccharide biosynthesis methods, e.g.,  Erwinia herbicola  ( Pantoea agglomerans ),  Citrobacter freundii, Pantoea citrea, Pectobacterium carotovorum , or  Xanthomonas campestris . Bacteria of the genus  Bacillus  may also be used, including  Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, Bacillus thermophilus, Bacillus laterosporus, Bacillus megaterium, Bacillus mycoides, Bacillus pumilus, Bacillus lentus, Bacillus cereus , and  Bacillus circulans . Similarly, bacteria of the genera  Lactobacillus  and  Lactococcus  may be modified using the methods of this invention, including but not limited to  Lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus helveticus, Lactobacillus delbrueckii, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus casei, Lactobacillus reuteri, Lactobacillus jensenii , and  Lactococcus lactis. Streptococcus thermophiles  and  Proprionibacterium freudenreichii  are also suitable bacterial species for the invention described herein. Also included as part of this invention are strains, modified as described here, from the genera  Enterococcus  (e.g.,  Enterococcus faecium  and  Enterococcus thermophiles ),  Bifidobacterium  (e.g.,  Bifidobacterium longum, Bifidobacterium infantis , and  Bifidobacterium bifidum ),  Sporolactobacillus  spp.,  Micromomospora  spp.,  Micrococcus  spp.,  Rhodococcus  spp., and  Pseudomonas  (e.g.,  Pseudomonas fluorescens  and  Pseudomonas aeruginosa ). Bacteria comprising the characteristics described herein are cultured in the presence of lactose, and a fucosylated oligosaccharide is retrieved, either from the bacterium itself or from a culture supernatant of the bacterium. The fucosylated oligosaccharide is purified for use in therapeutic or nutritional products, or the bacteria are used directly in such products. A suitable production host bacterial strain is one that is not the same bacterial strain as the source bacterial strain from which the fucosyltransferase-encoding nucleic acid sequence was identified. 
     Other features and advantages of the invention will be apparent from the following description of the preferred embodiments thereof, and from the claims. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All published foreign patents and patent applications cited herein are incorporated herein by reference. Genbank and NCBI submissions indicated by accession number cited herein are incorporated herein by reference. All other published references, documents, manuscripts and scientific literature cited herein are incorporated herein by reference. In the case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG.  1    is a schematic illustration showing the synthetic pathway of the major neutral fucosyl-oligosaccharides found in human milk. 
         FIG.  2    is a schematic demonstrating metabolic pathways and the changes introduced into them to engineer 2′-fucosyllactose (2′-FL) synthesis in  Escherichia coli  ( E. coli ). Specifically, the lactose synthesis pathway and the GDP-fucose synthesis pathway are illustrated. In the GDP-fucose synthesis pathway: manA=phosphomannose isomerase (PMI), manB=phosphomannomutase (PMM), manC=mannose-1-phosphate guanylyltransferase (GMP), gmd=GDP-mannose-4,6-dehydratase,fcl=GDP-fucose synthase (GFS), and Δwcaf=mutated UDP-glucose lipid carrier transferase. 
         FIG.  3 A  and  FIG.  3 B  show the sequence identity and a multiple sequence alignment of 4 previously known lactose-utilizing α(1,2)-fucosyltransferase protein sequences.  FIG.  3 A  is a table showing the sequence identity between the 4 known lactose-utilizing α(1,2)-fucosyltransferases:  H. pylori  futC (SEQ ID NO: 1),  H. mustelae  FutL (SEQ ID NO: 2),  Bacteroides vulgatus  futN (SEQ ID NO: 3), and  E. coli  0126 wbgL (SEQ ID NO: 4).  FIG.  3 B  shows multiple sequence alignment of the 4 known α(1,2)-fucosyltransferases. The ovals highlight regions of particularly high sequence conservation between the four enzymes in the alignment. 
         FIG.  4 A  through  FIG.  4 F  show the sequence alignment of the 12 identified α(1,2)-fucosyltransferase syngenes identified, along with the 4 previously known lactose-utilizing α(1,2)-fucosyltransferase protein sequences. The 4 known lactose-utilizing α(1,2)-fucosyltransferases are boxed and include  H. pylori  futC (SEQ ID NO: 1),  H. mustelae  FutL (SEQ ID NO: 2),  Bacteroides  vulgatus futN (SEQ ID NO: 3), and  E. coli  0126 wbgL (SEQ ID NO: 4). The 12 identified α(1,2)-fucosyltransferase are as follows:  Prevotella melaninogenica  FutO (SEQ ID NO: 10),  Clostridium bolteae+ 13 FutP (SEQ ID NO: 292),  Lachnospiraceae  sp. FutQ (SEQ ID NO: 12),  Methanosphaerula palustris  FutR (SEQ ID NO: 13), Tannerella sp. FutS (SEQ ID NO: 14),  Bacteroides  caccae FutU (SEQ ID NO: 15),  Butyrivibrio  FutV (SEQ ID NO: 16),  Prevotella  sp. FutW (SEQ ID NO: 17), Parabacteroides  johnsonii  FutX (SEQ ID NO: 18),  Akkermansia muciniphilia  FutY (SEQ ID NO: 19),  Salmonella enterica  FutZ (SEQ ID NO: 20),  Bacteroides  sp. FutZA (SEQ ID NO: 21). The sequence for  Clostridium bolteae  FutP (without the 13 additional amino acids in the N-terminus) (SEQ ID NO: 11) is also shown in the alignment. 
         FIG.  5 A  and  FIG.  5 B  are two pictures of gels showing the construction of the syngenes for each of the 12 novel α(1,2)-fucosyltransferases.  FIG.  5 A  shows post-Gibson assembly PCR.  FIG.  5 B  shows gel-purified RI/Xho1 syngene fragments. 
         FIG.  6 A  and  FIG.  6 B  are two photographs showing thin layer chromatograms of fucosylated oligosaccharide products produced in  E. coli  cultures using the 12 novel α(1,2)-fucosyltransferase syngenes.  FIG.  6 A  shows fucosylated oligosaccharide products from 2 μl of culture supernatant.  FIG.  6 B  shows fucosylated oligosaccharide products from 0.2 OD 600  cell equivalents of whole cell heat extracts. 
         FIG.  7    is a graph showing the growth curve of the host bacterium expressing plasmids containing the α(1,2) fucosyltransferase genes WbgL, FutN, FutO, FutQ, and FutX after tryptophan induction in the presence of lactose in the culture medium (i.e. lac+trp). 
         FIG.  8    is a photograph of a SDS-PAGE gel showing the proteins produced from host bacterium expressing α(1,2) fucosyltransferase genes WbgL, FutN, FutO, FutQ, and FutX after induction. 
         FIG.  9 A  and  FIG.  9 B  are two photographs of thin layer chromatograms showing the production of fucosylated oligosaccharide products from in  E. coli  cultures expressing select α(1,2)-fucosyltransferase syngenes WbgL, FutN, FutO, FutQ, and FutX at 7 hours or 24 hours after induction.  FIG.  9 A  shows fucosylated oligosaccharide products from 2 μl of culture supernatant.  FIG.  9 B  shows fucosylated oligosaccharide products from 0.2 OD 600  cell equivalents of whole cell heat extracts. 
         FIG.  10 A  and  FIG.  10 B  are two photographs of thin layer chromatograms showing the fucosylated oligosaccharide products after two different 1.5 L fermentation runs from  E. coli  expressing FutN:  FIG.  10 A )  36 B and  FIG.  10 B )  37 A. The culture yield for run  36 B was 33 g/L while the yield for run  37 A was 36.3 g/L. 
         FIG.  11    is a plasmid map of pG217 carrying the  B. vulgatus  FutN gene. 
         FIG.  12    is a schematic diagram showing the insertion of the LacIq promoter, the functional lacY gene, and the deletion of lacA. 
         FIG.  13    is a schematic diagram showing the deletion of the endogenous wcaJ gene using FRT recombination. 
         FIG.  14    is a schematic diagram of the  E. coli  W3110 chromosome, showing the insertion of a DNA fragment carrying kanamycin resistance gene (derived from transposon Tn5) and wild-type lacZ into the lon gene. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     While some studies suggest that human milk glycans could be used as antimicrobial anti-adhesion agents, the difficulty and expense of producing adequate quantities of these agents of a quality suitable for human consumption has limited their full-scale testing and perceived utility. What has been needed is a suitable method for producing the appropriate glycans in sufficient quantities at reasonable cost. Prior to the invention described herein, there were attempts to use several distinct synthetic approaches for glycan synthesis. Some chemical approaches can synthesize oligosaccharides (Flowers, H. M. Methods Enzymol 50, 93-121 (1978); Seeberger, P. H. Chem Commun (Camb) 1115-1121 (2003)), but reactants for these methods are expensive and potentially toxic (Koeller, K. M. &amp; Wong, C. H. Chem Rev 100, 4465-4494 (2000)). Enzymes expressed from engineered organisms (Albermann, C., Piepersberg, W. &amp; Wehmeier, U. F. Carbohydr Res 334, 97-103 (2001); Bettler, E., Samain, E., Chazalet, V., Bosso, C., et al. Glycoconj J 16, 205-212 (1999); Johnson, K. F. Glycoconj J 16, 141-146 (1999); Palcic, M. M. Curr Opin Biotechnol 10, 616-624 (1999); Wymer, N. &amp; Toone, E. J. Curr Opin Chem Biol 4, 110-119 (2000)) provide a precise and efficient synthesis (Palcic, M. M. Curr Opin Biotechnol 10, 616-624 (1999)); Crout, D. H. &amp; Vic, G. Curr Opin Chem Biol 2, 98-111 (1998)), but the high cost of the reactants, especially the sugar nucleotides, limits their utility for low-cost, large-scale production. Microbes have been genetically engineered to express the glycosyltransferases needed to synthesize oligosaccharides from the bacteria&#39;s innate pool of nucleotide sugars (Endo, T., Koizumi, S., Tabata, K., Kakita, S. &amp; Ozaki, A. Carbohydr Res 330, 439-443 (2001); Endo, T., Koizumi, S., Tabata, K. &amp; Ozaki, A. Appl Microbiol Biotechnol 53, 257-261 (2000); Endo, T. &amp; Koizumi, S. Curr Opin Struct Biol 10, 536-541 (2000); Endo, T., Koizumi, S., Tabata, K., Kakita, S. &amp; Ozaki, A. Carbohydr Res 316, 179-183 (1999); Koizumi, S., Endo, T., Tabata, K. &amp; Ozaki, A. Nat Biotechnol 16, 847-850 (1998)). However, prior to the invention described herein, there was a growing need to identify and characterize additional glycosyltransferases that are useful for the synthesis of HMOS in metabolically engineered bacterial hosts. 
     Human Milk Glycans 
     Human milk contains a diverse and abundant set of neutral and acidic oligosaccharides (Kunz, C., Rudloff, S., Baier, W., Klein, N., and Strobel, S. (2000). Annu Rev Nutr 20, 699-722; Bode, L. (2006). J Nutr 136, 2127-130). More than 130 different complex oligosaccharides have been identified in human milk, and their structural diversity and abundance is unique to humans. Although these molecules may not be utilized directly by infants for nutrition, they nevertheless serve critical roles in the establishment of a healthy gut microbiome (Marcobal, A., Barboza, M., Froehlich, J. W., Block, D. E., et al. J Agric Food Chem 58, 5334-5340 (2010)), in the prevention of disease (Newburg, D. S., Ruiz-Palacios, G. M. &amp; Morrow, A. L. Annu Rev Nutr 25, 37-58 (2005)), and in immune function (Newburg, D. S. &amp; Walker, W. A. Pediatr Res 61, 2-8 (2007)). Despite millions of years of exposure to human milk oligosaccharides (HMOS), pathogens have yet to develop ways to circumvent the ability of HMOS to prevent adhesion to target cells and to inhibit infection. The ability to utilize HMOS as pathogen adherence inhibitors promises to address the current crisis of burgeoning antibiotic resistance. Human milk oligosaccharides produced by biosynthesis represent the lead compounds of a novel class of therapeutics against some of the most intractable scourges of society. 
     One alternative strategy for efficient, industrial-scale synthesis of HMOS is the metabolic engineering of bacteria. This approach involves the construction of microbial strains overexpressing heterologous glycosyltransferases, membrane transporters for the import of precursor sugars into the bacterial cytosol, and possessing enhanced pools of regenerating nucleotide sugars for use as biosynthetic precursors (Dumon, C., Samain, E., and Priem, B. (2004). Biotechnol Prog 20, 412-19; Ruffing, A., and Chen, R. R. (2006). Microb Cell Fact 5, 25). A key aspect of this approach is the heterologous glycosyltransferase selected for overexpression in the microbial host. The choice of glycosyltransferase can significantly affect the final yield of the desired synthesized oligosaccharide, given that enzymes can vary greatly in terms of kinetics, substrate specificity, affinity for donor and acceptor molecules, stability and solubility. A few glycosyltransferases derived from different bacterial species have been identified and characterized in terms of their ability to catalyze the biosynthesis of HMOS in  E. coli  host strains (Dumon, C., Bosso, C., Utille, J. P., Heyraud, A., and Samain, E. (2006). Chembiochem 7, 359-365; Dumon, C., Samain, E., and Priem, B. (2004). Biotechnol Prog 20, 412-19; Li, M., Liu, X. W., Shao, J., Shen, J., Jia, Q., Yi, W., Song, J. K., Woodward, R., Chow, C. S., and Wang, P. G. (2008). Biochemistry 47, 378-387). The identification of additional glycosyltransferases with faster kinetics, greater affinity for nucleotide sugar donors and/or acceptor molecules, or greater stability within the bacterial host significantly improves the yields of therapeutically useful HMOS. Prior to the invention described herein, chemical syntheses of HMOS were possible, but were limited by stereo-specificity issues, precursor availability, product impurities, and high overall cost (Flowers, H. M. Methods Enzymol 50, 93-121 (1978); Seeberger, P. H. Chem Commun (Camb) 1115-1121 (2003); Koeller, K. M. &amp; Wong, C. H. Chem Rev 100, 4465-4494 (2000)). The invention overcomes the shortcomings of these previous attempts by providing new strategies to inexpensively manufacture large quantities of human milk oligosaccharides (HMOS) for use as dietary supplements. Advantages include efficient expression of the enzyme, improved stability and/or solubility of the fucosylated oligosaccharide product (2′-FL, LDFT, LNF I, and LDFH I) and reduced toxicity to the host organism. The present invention features novel α(1,2) FTs suitable for expression in production strains for increased efficacy and yield of fucosylated HMOS compared to α(1,2) FTs currently utilized in the field. 
     As described in detail below,  E. coli  (or other bacteria) is engineered to produce selected fucosylated oligosaccharides (i.e., 2′-FL, LDFT, LDHF I, or LNF I) in commercially viable levels. For example, yields are &gt;5 grams/liter in a bacterial fermentation process. In other embodiments, the yields are greater than 10 grams/liter, greater than 15 grams/liter, greater than 20 grams/liter, greater than 25 grams/liter, greater than 30 grams/liter, greater than 35 grams/liter, greater than 40 grams/liter, greater than 45 grams/liter, greater than 50 grams/liter, greater than 55 grams/liter, greater than 60 grams/liter, greater than 65 grams/liter, greater than 70 grams/liter, or greater than 75 grams/liter of fucosylated oligosaccharide products, such as 2′-FL, LDFT, LDHF I, and LNF I. 
     Role of Human Milk Glycans in Infectious Disease 
     Human milk glycans, which comprise both unbound oligosaccharides and their glycoconjugates, play a significant role in the protection and development of the infant gastrointestinal (GI) tract. Neutral fucosylated oligosaccharides, including 2′-fucosyllactose (2′-FL), protect infants against several important pathogens. Milk oligosaccharides found in various mammals differ greatly, and the composition in humans is unique (Hamosh M., 2001 Pediatr Clin North Am, 48:69-86; Newburg D. S., 2001 Adv Exp Med Biol, 501:3-10). Moreover, glycan levels in human milk change throughout lactation and also vary widely among individuals (Morrow A. L. et al., 2004 J Pediatr, 145:297-303; Chaturvedi P et al., 2001 Glycobiology, 11:365-372). Approximately 200 distinct human milk oligosaccharides have been identified and combinations of simple epitopes are responsible for this diversity (Newburg D. S., 1999 Curr Med Chem, 6:117-127; Ninonuevo M. et al., 2006 J Agric Food Chem, 54:7471-74801). 
     Human milk oligosaccharides are composed of 5 monosaccharides: D-glucose (Glc), D-galactose (Gal), N-acetylglucosamine (GlcNAc), L-fucose (Fuc), and sialic acid (N-acetyl neuraminic acid, NeuSAc, NANA). Human milk oligosaccharides are usually divided into two groups according to their chemical structures: neutral compounds containing Glc, Gal, GlcNAc, and Fuc, linked to a lactose (Galβ1-4G1c) core, and acidic compounds including the same sugars, and often the same core structures, plus NANA (Charlwood J. et al., 1999 Anal Biochem, 273:261-277; Martin-Sosa et al., 2003 J Dairy Sci, 86:52-59; Parkkinen J. and Finne J., 1987 Methods Enzymol, 138:289-300; Shen Z. et al., 2001 J Chromatogr A, 921:315-321). 
     Approximately 70-80% of oligosaccharides in human milk are fucosylated, and their synthetic pathways are believed to proceed as shown in  FIG.  1   . A smaller proportion of the oligosaccharides are sialylated or both fucosylated and sialylated, but their synthetic pathways are not fully defined. Understanding of the acidic (sialylated) oligosaccharides is limited in part by the ability to measure these compounds. Sensitive and reproducible methods for the analysis of both neutral and acidic oligosaccharides have been designed. Human milk oligosaccharides as a class survive transit through the intestine of infants very efficiently, being essentially indigestible (Chaturvedi, P., Warren, C. D., Buescher, C. R., Pickering, L. K. &amp; Newburg, D. S. Adv Exp Med Biol 501, 315-323 (2001)). 
     Human Milk Glycans Inhibit Binding of Enteropathogens to their Receptors 
     Human milk glycans have structural homology to cell receptors for enteropathogens and function as receptor decoys. For example, pathogenic strains of  Campylobacter  bind specifically to glycans containing H-2, i.e., 2′-fucosyl-N-acetyllactosamine or 2′-fucosyllactose (2′FL);  Campylobacter  binding and infectivity are inhibited by 2′-FL and other glycans containing this H-2 epitope. Similarly, some diarrheagenic  E. coli  pathogens are strongly inhibited in vivo by human milk oligosaccharides containing 2-linked fucose moieties. Several major strains of human caliciviruses, especially the noroviruses, also bind to 2-linked fucosylated glycans, and this binding is inhibited by human milk 2-linked fucosylated glycans. Consumption of human milk that has high levels of these 2-linked fucosyloligosaccharides was associated with lower risk of norovirus,  Campylobacter , ST of  E. coli -associated diarrhea, and moderate-to-severe diarrhea of all causes in a Mexican cohort of breastfeeding children (Newburg D. S. et al., 2004 Glycobiology, 14:253-263; Newburg D. S. et al., 1998 Lancet, 351:1160-1164). Several pathogens utilize sialylated glycans as their host receptors, such as influenza (Couceiro, J. N., Paulson, J. C. &amp; Baum, L. G. Virus Res 29, 155-165 (1993)), parainfluenza (Amonsen, M., Smith, D. F., Cummings, R. D. &amp; Air, G. M. J Virol 81, 8341-8345 (2007), and rotoviruses (Kuhlenschmidt, T. B., Hanafin, W. P., Gelberg, H. B. &amp; Kuhlenschmidt, M. S. Adv Exp Med Biol 473, 309-317 (1999)). The sialyl-Lewis X epitope is used by  Helicobacter pylori  (Mandavi, J., Sondén, B., Hurtig, M., Olfat, F. O., et al. Science 297, 573-578 (2002)),  Pseudomonas aeruginosa  (Scharfman, A., Delmotte, P., Beau, J., Lamblin, G., et al. Glycoconj J 17, 735-740 (2000)), and some strains of noroviruses (Rydell, G. E., Nilsson, J., Rodriguez-Diaz, J., Ruvoen-Clouet, N., et al. Glycobiology 19, 309-320 (2009)). 
     Identification of Novel α(1,2) Fucosyltransferases 
     The present invention provides novel α(1,2) fucosyltransferase enzymes. The present invention also provides nucleic acid constructs (i.e., a plasmid or vector) carrying the nucleic acid sequence of a novel α(1,2) fucosyltransferases for the expression of the novel α(1,2) fucosyltransferases in host bacterium. The present invention also provides methods for producing fucosylated oligosaccharides by expressing the novel α(1,2) fucosyltransferases in suitable host production bacterium, as further described herein. 
     Not all α(1,2)fucosyltransferases can utilize lactose as an acceptor substrate. An acceptor substrate includes, for example, a carbohydrate, an oligosaccharide, a protein or glycoprotein, a lipid or glycolipid, e.g., N-acetylglucosamine, N-acetyllactosamine, galactose, fucose, sialic acid, glucose, lactose, or any combination thereof. A preferred alpha (1,2) fucosyltransferase of the present invention utilizes GDP-fucose as a donor, and lactose is the acceptor for that donor. 
     A method of identifying novel α(1,2)fucosyltransferase enzymes capable of utilizing lactose as an acceptor was previously carried out (as described in PCT/US2013/051777, hereby incorporated by reference in its entirety) using the following steps: 1) performing a computational search of sequence databases to define a broad group of simple sequence homologs of any known, lactose-utilizing α(1,2)fucosyltransferase; 2) using the list of homologs from step 1 to derive a search profile containing common sequence and/or structural motifs shared by the members of the broad group, e.g. by using computer programs such as MEME (Multiple Em for Motif Elicitation available at http://meme.sdsc.edu/meme/cgi-bin/meme.cgi) or PSI-BLAST (Position-Specific Iterated BLAST available at ncbi.nlm.nih.gov/blast with additional information at cnx.org/content/m11040/latest/); 3) searching sequence databases (e.g., using computer programs such as PSI-BLAST, or MAST (Motif Alignment Search Tool available at http://meme.sdsc.edu/meme/cgi-bin/mast.cgi); using this derived search profile as query, and identifying “candidate sequences” whose simple sequence homology to the original lactose-accepting α(1,2)fucosyltransferase is 40% or less; 4) scanning the scientific literature and developing a list of “candidate organisms” known to express α(1,2)fucosyl-glycans; 5) selecting only those “candidate sequences” that are derived from “candidate organisms” to generate a list of “candidate lactose-utilizing enzymes”; and 6) expressing each “candidate lactose-utilizing enzyme” and testing for lactose-utilizing α(1,2)fucosyltransferase activity. 
     The MEME suite of sequence analysis tools (meme.sdsc.edu/meme/cgi-bin/meme.cgi) can also be used as an alternative to PSI-BLAST. Sequence motifs are discovered using the program “MEME”. These motifs can then be used to search sequence databases using the program “MAST”. The BLAST and PSI-BLAST search algorithms are other well known alternatives. 
     To identify additional novel α(1,2)fucosyltransferases, a multiple sequence alignment query was generated using four previously identified lactose-utilizing α(1,2)fucosyltransferase protein sequences:  H. pylori  futC (SEQ ID NO: 1),  H. mustelae  FutL (SEQ ID NO: 2),  Bacteroides  vulgatus futN (SEQ ID NO: 3), and  E. coli  0126 wbgL (SEQ ID NO: 4). These sequence alignment and percentage of sequence identity is shown in  FIG.  3   . An iterative PSI-BLAST was performed, using the FASTA-formatted multiple sequence alignment as the query, and the NCBI PSI-BLAST program run on a local copy of NCBI BLAST+ version 2.2.29. An initial position-specific scoring matrix file (.pssm) was generated by PSI-BLAST, which the program then used to adjust the score of iterative homology search runs. The process is iterated to generate an even larger group of candidates, and the results of each run were used to further refine the matrix. 
     This PSI-BLAST search resulted in an initial 2515 hits. There were 787 hits with greater than 22% sequence identity to FutC. 396 hits were of greater than 275 amino acids in length. Additional analysis of the hits was performed, including sorting by percentage identity to FutC, comparing the sequences by BLAST to existing α(1,2) fucosyltransferase inventory (of known α(1,2) fucosyltransferases), and manual annotation of hit sequences to identify those originating from bacteria that naturally exist in the gastrointestinal tract. An annotated list of the novel α(1,2) fucosyltransferases identified by this screen are listed in Table 1. Table 1 provides the bacterial species from which the candidate enzyme is found, the GenBank Accession Number, GI Identification Number, amino acid sequence, and % sequence identity to FutC. 
     Of the identified hits, 12 novel α(1,2) fucosyltransferases were further analyzed for their functional capacity:  Prevotella melaninogenica  FutO,  Clostridium bolteae  FutP,  Clostridium bolteae+ 13 FutP,  Lachnospiraceae  sp. FutQ,  Methanosphaerula palustries  FutR,  Tannerella  sp. FutS,  Bacteroides  caccae FutU,  Butyrivibrio  FutV,  Prevotellaa  sp. FutW,  Parabacteroides johnsonii  FutX,  Akkermansia muciniphilia  FutY,  Salmonella enterica  FutZ, and  Bacteroides  sp. FutZA. For  Clostridium bolteae  FutP, the annotation named the wrong initiation methionine codon. Thus, the present invention includes FutP with an additional 13 amino acids at the N-terminus of the annotated FutP (derived in-frame from the natural upstream DNA sequence), which is designated herein as  Clostridium bolteae+ 13 FutP. The sequence identity between the 12 novel α(1,2) fucosyltransferases identified and the 4 previously identified α(1,2) fucosyltransferases is shown in Table 2 below. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Sequence Identity 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
                 7 
                 8 
                 9 
                 10 
                 11 
                 12 
                 13 
                 14 
                 15 
                 16 
               
               
                   
               
               
                   H. pylori  futC 
                  1 
                   
                 70.10 
                 21.99 
                 20.82 
                 27.68 
                 27.36 
                 23.56 
                 23.28 
                 23.62 
                 25.75 
                 23.72 
                 24.05 
                 22.29 
                 24.19 
                 22.92 
                 22.29 
               
               
                   H. mustelae  futL 
                  2 
                 70.10 
                   
                 23.87 
                 19.88 
                 26.38 
                 28.21 
                 24.30 
                 23.38 
                 24.62 
                 25.31 
                 25.31 
                 24.47 
                 23.56 
                 25.15 
                 23.55 
                 23.26 
               
               
                 
                   Bacteroides vulgatus 
                 
                  3 
                 21.99 
                 23.87 
                   
                 25.16 
                 32.05 
                 28.71 
                 28.94 
                 25.79 
                 37.46 
                 32.27 
                 26.11 
                 61.27 
                 71.63 
                 27.67 
                 25.15 
                 84.75 
               
               
                 futN 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   E. coli  0126 wbgL 
                  4 
                 20.82 
                 19.88 
                 25.16 
                   
                 24.25 
                 22.73 
                 22.32 
                 26.04 
                 25.45 
                 24.77 
                 21.49 
                 23.29 
                 26.71 
                 24.63 
                 21.45 
                 25.16 
               
               
                 
                   Prevotella 
                 
                  5 
                 27.68 
                 26.38 
                 32.05 
                 24.25 
                   
                 36.96 
                 31.63 
                 35.74 
                 35.16 
                 55.74 
                 30.28 
                 30.03 
                 32.80 
                 30.09 
                 26.28 
                 31.83 
               
               
                   melaninogenica  Fut0 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 YP_003814512.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Clostridium  
                 
                  6 
                 27.36 
                 28.21 
                 28.71 
                 22.73 
                 36.96 
                   
                 37.87 
                 35.10 
                 33.77 
                 36.91 
                 35.74 
                 29.58 
                 31.39 
                 27.67 
                 26.33 
                 29.13 
               
               
                   bolteae  + 13 FutP 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 WP_002570768.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   Lachnospiraceae  sp. 
                  7 
                 23.56 
                 24.30 
                 28.94 
                 22.32 
                 31.63 
                 37.87 
                   
                 29.87 
                 29.17 
                 32.90 
                 51.02 
                 28.53 
                 30.00 
                 27.69 
                 24.00 
                 27.74 
               
               
                 FutQ 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 WP_009251343.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Methanosphaerula 
                 
                  8 
                 23.28 
                 23.38 
                 25.79 
                 26.04 
                 35.74 
                 35.10 
                 29.87 
                   
                 28.71 
                 38.24 
                 31.41 
                 25.39 
                 28.08 
                 30.65 
                 23.93 
                 25.55 
               
               
                   palustris  FutR 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 YP_002467213.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   Tannerella  sp. FutS 
                  9 
                 23.62 
                 24.62 
                 37.46 
                 25.45 
                 35.16 
                 33.77 
                 29.17 
                 28.71 
                   
                 34.41 
                 30.03 
                 35.71 
                 36.27 
                 26.48 
                 21.75 
                 36.60 
               
               
                 WP_021929367.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides caccae 
                 
                 10 
                 25.75 
                 25.31 
                 32.27 
                 24.77 
                 55.74 
                 36.91 
                 32.90 
                 38.24 
                 34.41 
                   
                 31.21 
                 29.94 
                 33.33 
                 29.28 
                 24.46 
                 33.01 
               
               
                 FutU 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 WP_005675707.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   Butyrivibrio  FutV 
                 11 
                 23.72 
                 25.31 
                 26.11 
                 21.49 
                 30.28 
                 35.74 
                 51.02 
                 31.41 
                 30.03 
                 31.21 
                   
                 27.62 
                 26.20 
                 26.46 
                 22.15 
                 26.52 
               
               
                 WP_022772718.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   Prevotella  sp. FutW 
                 12 
                 24.05 
                 24.47 
                 61.27 
                 23.29 
                 30.03 
                 29.58 
                 28.53 
                 25.39 
                 35.71 
                 29.94 
                 27.62 
                   
                 57.60 
                 25.79 
                 22.15 
                 59.01 
               
               
                 WP_022481266.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Parabacteroides 
                 
                 13 
                 22.29 
                 23.56 
                 71.63 
                 26.71 
                 32.80 
                 31.39 
                 30.00 
                 28.08 
                 36.27 
                 33.33 
                 26.20 
                 57.60 
                   
                 28.71 
                 24.00 
                 74.02 
               
               
                   johnsonii  FutX 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 WP_008155883.1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Akkermansia 
                 
                 14 
                 24.19 
                 25.15 
                 27.67 
                 24.63 
                 30.09 
                 27.67 
                 27.69 
                 30.65 
                 26.48 
                 29.28 
                 26.46 
                 25.79 
                 28.71 
                   
                 21.45 
                 28.08 
               
               
                   muciniphilia  FutY 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 YP_001877555 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Salmonella enterica 
                 
                 15 
                 22.92 
                 23.55 
                 25.15 
                 21.45 
                 26.28 
                 26.33 
                 24.00 
                 23.93 
                 21.75 
                 24.46 
                 22.15 
                 22.15 
                 24.00 
                 21.45 
                   
                 24.62 
               
               
                 FutZ 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 WP_023214330 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   Bacteroides  sp. 
                 16 
                 22.29 
                 23.26 
                 84.75 
                 25.16 
                 31.83 
                 29.13 
                 27.74 
                 25.55 
                 36.60 
                 33.01 
                 26.52 
                 59.01 
                 74.02 
                 28.08 
                 24.62 
                   
               
               
                 FutZA 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 WP_022161880.1 
               
               
                   
               
            
           
         
       
     
     Based on the amino acid sequences of the identified α(1,2) fucosyltransferases (i.e., in Table 1), syngenes can be readily designed and constructed by the skilled artisan using standard methods known in the art. For example, the syngenes include a ribosomal binding site, are codon-optimized for expression in a host bacterial production strain (i.e.,  E. coli ), and have common 6-cutter restriction sites or sites recognized by endogenous restriction enzymes present in the host strain (i.e., EcoK restriction sites) removed to ease cloning and expression in the  E. coli  host strain. In a preferred embodiment, the syngenes are constructed with the following configuration: EcoRI site-T7g10 RBS-α(1,2) FT syngene-XhoI site. The nucleic acid sequences of sample syngenes for the 12 identified α(1,2) fucosyltransferases are shown in Table 3. (the initiating methionine ATG codon is bolded) 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Nucleic acid sequences of 12 novel α(1,2) fucosyltransferase syngenes 
               
            
           
           
               
               
               
            
               
                 Bacteria/ 
                   
                 SEQ 
               
               
                 Gene 
                   
                 ID 
               
               
                 name 
                 Sequence 
                 NO: 
               
               
                   
               
               
                 FutO 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG AAAATCGTCAAAATCCTGGGCGGT 
                 276 
               
               
                   
                 CTGGGCAATCAGATGTTCCAGTATGCTCTGTACCTGAGCCTGCAAGAAAGTTTTCCAAAA 
                   
               
               
                   
                 GAACGTGTGGCCCTGGACCTGTCCTCCTTCCACGGCTATCACCTGCATAATGGCTTTGAG 
                   
               
               
                   
                 CTGGAGAACATTTTCTCCGTTACCGCTCAGAAAGCATCCGCCGCAGATATCATGCGTATT 
                   
               
               
                   
                 GCTTATTACTACCCGAACTATCTGCTGTGGCGCATTGGCAAACGTTTTCTGCCGCGTCGT 
                   
               
               
                   
                 AAAGGTATGTGCCTGGAATCTAGCTCCCTGCGTTTCGATGAAAGCGTTCTGCGTCAGGAA 
                   
               
               
                   
                 GGTAACCGTTATTTTGACGGTTACTGGCAAGACGAACGCTACTTCGCAGCCTATCGTGAA 
                   
               
               
                   
                 AAAGTGCTGAAGGCTTTCACCTTTCCTGCATTCAAACGCGCAGAAAACCTGAGCCTGCTG 
                   
               
               
                   
                 GAAAAACTGGACGAAAACAGCATTGCTCTGCATGTTCGTCGCGGTGATTACGTAGGTAAT 
                   
               
               
                   
                 AACCTGTACCAAGGCATCTGTGACCTGGACTACTACCGTACCGCTATCGAGAAAATGTGT 
                   
               
               
                   
                 GCACACGTTACTCCGTCTCTGTTTTGTATCTTTTCCAACGACATCACGTGGTGCCAGCAG 
                   
               
               
                   
                 CACCTGCAACCGTACCTGAAGGCCCCTGTGGTGTACGTTACTTGGAACACCGGTGTTGAA 
                   
               
               
                   
                 TCCTACCGCGATATGCAGCTGATGTCCTGCTGCGCACATAACATCATCGCGAATAGCTCC 
                   
               
               
                   
                 TTCTCTTGGTGGGGTGCTTGGCTGAATCAGAACCGTGAAAAAGTTGTTATCGCCCCGAAA 
                   
               
               
                   
                 AAATGGCTGAACATGGAAGAATGTCACTTCACGCTGCCGGCAAGCTGGATCAAAATTTAG 
                   
               
               
                   
                 CTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutP 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG GTGATTATCAAAATGATGGGTGGT 
                 277 
               
               
                   
                 CTGGGCAACCAGATGTTCCAGTACGCACTGTACAAAGCATTCGAGCAGAAGCACATCGAT 
                   
               
               
                   
                 GTGTATGCAGACCTGGCATGGTACAAAAACAAATCCGTGAAATTTGAACTGTACAACTTC 
                   
               
               
                   
                 GGCATTAAAATCAACGTAGCATCCGAGAAAGACATCAACCGTCTGAGCGATTGCCAGGCG 
                   
               
               
                   
                 GACTTTGTTTCCCGCATCCGCCGTAAAATCTTTGGTAAAAAAAAGAGCTTCGTATCTGAA 
                   
               
               
                   
                 AAAAATGACTCCTGCTATGAAAACGACATCCTGCGTATGGACAACGTTTATCTGAGCGGT 
                   
               
               
                   
                 TATTGGCAGACCGAAAAATACTTCTCTAACACGCGTGAGAAGCTGCTGGAGGATTATTCC 
                   
               
               
                   
                 TTCGCTCTGGTAAACTCTCAGGTGTCCGAATGGGAAGACTCCATTCGCAACAAAAACAGC 
                   
               
               
                   
                 GTTAGCATCCATATCCGTCGTGGTGATTATCTACAGGGCGAACTGTATGGTGGTATTTGC 
                   
               
               
                   
                 ACCTCTCTGTACTACGCCGAAGCAATCGAGTACATTAAAATGCGTGTTCCGAACGCAAAA 
                   
               
               
                   
                 TTCTTCGTTTTCTCTGATGACGTTGAATGGGTTAAACAGCAAGAAGACTTCAAAGGCTTC 
                   
               
               
                   
                 GTAATCGTTGATCGCAACGAGTATTCTAGCGCTCTGTCCGATATGTACCTGATGTCCCTG 
                   
               
               
                   
                 TGCAAGCATAACATTATTGCTAACTCCTCTTTCAGCTGGTGGGCAGCTTGGCTGAACCGT 
                   
               
               
                   
                 AACGAAGAAAAAATTGTAATCGCGCCGCGCCGTTGGCTGAACGGCAAGTGCACCCCAGAT 
                   
               
               
                   
                 ATCTGGTGTAAAAAATGGATTCGTATCTAGCTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutQ 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG GTGATCGTACAGCTGAGCGGCGGT 
                 278 
               
               
                   
                 CTGGGCAACCAGATGTTCGAATACGCGCTGTACCTGAGCCTGAAAGCAAAAGGCAAAGAA 
                   
               
               
                   
                 GTGAAAATTGACGATGTTACGTGTTACGAGGGCCCTGGCACCCGTCCGCGTCAACTGGAT 
                   
               
               
                   
                 GTTTTTGGTATCACGTACGATCGCGCGTCTCGTGAGGAGCTGACTGAGATGACGGACGCG 
                   
               
               
                   
                 AGCATGGATGCGCTGTCTCGTGTTCGTCGCAAACTGACCGGTCGCCGCACTAAAGCGTAC 
                   
               
               
                   
                 CGCGAACGCGACATCAACTTCGATCCACTGGTTATGGAAAAAGACCCGGCACTGCTGGAA 
                   
               
               
                   
                 GGCTGTTTCCAGTCTGACAAATACTTTCGTGATTGCGAAGGCCGCGTGCGCGAAGCGTAT 
                   
               
               
                   
                 CGTTTCCGCGGCATTGAATCCGGCGCGTTCCCGCTGCCGGAAGACTATCTGCGCCTGGAA 
                   
               
               
                   
                 AAGCAGATCGAAGATTGTCAGTCCGTATCCGTACACATCCGTCGTGGCGACTACCTGGAC 
                   
               
               
                   
                 GAATCTCATGGTGGTCTGTACACCGGCATTTGTACTGAGGCGTACTATAAAGAGGCTTTT 
                   
               
               
                   
                 GCTCGCATGGAACGTCTGGTTCCGGGCGCACGTTTCTTCCTGTTCTCTAACGATCCAGAA 
                   
               
               
                   
                 TGGACTCGTGAGCACTTTGAGAGCAAGAACTGCGTTCTGGTTGAAGGTAGCACCGAAGAC 
                   
               
               
                   
                 ACGGGTTACATGGACCTGTACCTGATGAGCCGCTGCCGCCACAATATTATTGCCAACTCT 
                   
               
               
                   
                 TCTTTCAGCTGGTGGGGCGCTTGGCTGAATGAGAACCCTGAGAAAAAAGTCATCGCACCG 
                   
               
               
                   
                 GCTAAATGGCTGAACGGTCGTGAGTGCCGTGATATCTATACCGAACGCATGATTCGTCTG 
                   
               
               
                   
                 TAGCTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutR 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG ATCATTGTTCGTCTGAAAGGCGGT 
                 279 
               
               
                   
                 CTGGGCAACCAACTGTCTCAGTATGCACTGGGCCGTAAGATCGCGCATCTGCACAATACC 
                   
               
               
                   
                 GAACTGAAACTGGACACCACTTGGTTCACCACTATCTCCTCCGACACTCCACGTACCTAC 
                   
               
               
                   
                 CGTCTGAACAATTATAACATCATCGGCACTATTGCATCCGCAAAGGAAATCCAGCTGATC 
                   
               
               
                   
                 GAACGTGGTCGCGCGCAAGGCCGTGGCTACCTGCTGTCTAAAATTTCTGATCTGCTGACT 
                   
               
               
                   
                 CCGATGTACCGTCGTACCTACGTCCGTGAACGTATGCATACCTTCGATAAAGCTATCCTG 
                   
               
               
                   
                 ACCGTTCCGGACAACGTGTACCTGGATGGTTACTGGCAGACCGAAAAGTACTTCAAAGAC 
                   
               
               
                   
                 ATCGAAGAAATCCTGCGCCGTGAGGTTACGCTGAAAGATGAACCGGATAGCATCAACCTG 
                   
               
               
                   
                 GAAATGGCTGAACGTATTCAGGCTTGCCACAGCGTTTCCCTGCACGTGCGTCGTGGCGAC 
                   
               
               
                   
                 TACGTTTCCAACCCGACCACTCAACAATTCCACGGCTGTTGCTCCATTGACTACTACAAC 
                   
               
               
                   
                 CGCGCTATCTCTCTGATTGAAGAAAAAGTGGATGACCCGTCTTTCTTTATTTTTTCTGAC 
                   
               
               
                   
                 GATCTGCCGTGGGCTAAAGAAAACCTGGACATCCCTGGCGAAAAAACCTTCGTTGCGCAT 
                   
               
               
                   
                 AACGGCCCGGAAAAAGAGTATTGCGATCTGTGGCTGATGTCTCTGTGCCAGCACCATATC 
                   
               
               
                   
                 ATCGCAAACTCTTCTTTCAGCTGGTGGGGTGCCTGGCTGGGTCAAGACGCCGAAAAGATG 
                   
               
               
                   
                 GTGATCGCGCCGCGTCGCTGGGCCCTGTCCGAGAGCTTTGACACTTCTGACATCATTCCG 
                   
               
               
                   
                 GACTCTTGGATTACTATCTAGCTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutS 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG GTACGCATTGTGGAAATCATCGGC 
                 280 
               
               
                   
                 GGTCTGGGTAACCAGATGTTCCAGTACGCATTCTCCCTGTACCTGAAAAACAAATCTCAC 
                   
               
               
                   
                 ATCTGGGACCGTCTGTATGTGGACATCGAGGCGATGAAAACCTACGATCGTCACTATGGT 
                   
               
               
                   
                 CTGGAACTGGAGAAAGTTTTCAATCTGAGCCTGTGTCCAATCTCTAACCGTCTGCACCGC 
                   
               
               
                   
                 AACCTGCAAAAACGCTCCTTCGCAAAACACTTTGTAAAGAGCCTGTACGAGCACTCTGAA 
                   
               
               
                   
                 TGCGAGTTCGACGAACCGGTGTACCGTGGCCTGCGTCCTTATCGCTATTATCGCGGCTAC 
                   
               
               
                   
                 TGGCAAAACGAAGGTTACTTCGTTGATATTGAACCGATGATCCGTGAGGCTTTTCAGTTC 
                   
               
               
                   
                 AACGTTAACATCCTGAGCAAAAAGACTAAAGCGATCGCATCCAAAATGCGCCGTGAACTG 
                   
               
               
                   
                 TCCGTATCTATCCATGTTCGCCGTGGTGATTACGAAAACCTGCCGGAAGCGAAAGCGATG 
                   
               
               
                   
                 CATGGCGGTATTTGTTCTCTGGACTATTACCACAAAGCGATCGACTTCATCCGCCAGCGT 
                   
               
               
                   
                 CTGGATAATAACATCTGTTTCTATCTGTTCTCCGACGATATCAATTGGGTAGAAGAAAAC 
                   
               
               
                   
                 CTGCAACTGGAAAACCGTTGCATCATCGACTGGAACCAGGGCGAAGATAGCTGGCAGGAC 
                   
               
               
                   
                 ATGTACCTGATGAGCTGCTGCCGCCACCACATTATCGCAAACAGCTCTTTCTCCTGGTGG 
                   
               
               
                   
                 GCGGCATGGCTGAATCCAAACAAGAACAAAATCGTACTGACCCCGAACAAATGGTTCAAC 
                   
               
               
                   
                 CATACTGACGCAGTGGGTATCGTCCCAAAGTCCTGGATTAAAATTCCTGTGTTTTAGCTC 
                   
               
               
                   
                 GAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutU 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG AAAATCGTTAAAATCCTGGGCGGC 
                 281 
               
               
                   
                 CTGGGTAACCAGATGTTTCAGTACGCCCTGTTCCTGTCTCTGAAAGAACGCTTCCCGCAT 
                   
               
               
                   
                 GAACAGGTGATGATTGACACCAGCTGCTTCCGCAATTACCCACTGCACAACGGTTTCGAA 
                   
               
               
                   
                 GTGGATCGTATCTTCGCCCAGAAAGCACCGGTTGCCTCTTGGCGTAACATCCTGAAGGTT 
                   
               
               
                   
                 GCCTACCCGTACCCGAACTACCGTTTCTGGAAAATCGGTAAATACATCCTGCCTAAACGT 
                   
               
               
                   
                 AAAACCATGTGTGTAGAGCGTAAAAACTTCAGCTTTGACGCCGCAGTCCTGACCCGTAAA 
                   
               
               
                   
                 GGCGATTGCTACTATGATGGCTACTGGCAGCATGAGGAATATTTCTGTGATATGAAAGAA 
                   
               
               
                   
                 ACGATTTGGGAGGCTTTCTCCTTCCCTGAGCCGGTTGATGGTCGTAACAAGGAGATCGGT 
                   
               
               
                   
                 GCCCTGCTACAGGCATCTGATAGCGCTTCCCTGCACGTTCGTCGCGGTGACTACGTGAAC 
                   
               
               
                   
                 CACCCACTGTTTCGTGGTATTTGTGACCTGGACTATTATAAACGTGCCATCCACTACATG 
                   
               
               
                   
                 GAAGAACGCGTCAACCCACAGCTGTACTGCGTTTTCAGCAACGATATGGCCTGGTGCGAG 
                   
               
               
                   
                 TCCCACCTGCGTGCACTGCTGCCAGGCAAAGAAGTAGTTTATGTTGACTGGAACAAGGGT 
                   
               
               
                   
                 GCGGAATCTTACGTTGATATGCGTCTGATGAGCCTGTGCCGTCACAACATCATCGCTAAC 
                   
               
               
                   
                 TCTTCTTTCAGCTGGTGGGGCGCATGGCTGAACCGTAACCCGCAGAAAGTGGTGGTAGCG 
                   
               
               
                   
                 CCGGAACGTTGGATGAACAGCCCGATTGAAGACCCAGTGAGCGACAAATGGATTAAACTG 
                   
               
               
                   
                 TAGCTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutV 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG ATCATCATCCAGCTGAAAGGTGGC 
                 282 
               
               
                   
                 CTGGGCAACCAAATGTTCCAGTACGCGCTGTACAAATCCCTGAAAAAACGTGGTAAAGAA 
                   
               
               
                   
                 GTTAAAATTGATGACAAAACTGGCTTCGTGAACGACAAACTGCGTATCCCGGTACTGTCC 
                   
               
               
                   
                 CGTTGGGGTGTTGAGTACGATCGTGCAACCGACGAAGAGATTATTAACCTGACCGACTCC 
                   
               
               
                   
                 AAAATGGACCTGTTCTCTCGCATCCGCCGTAAACTGACTGGCCGCAAAACGTTCCGTATC 
                   
               
               
                   
                 GACGAAGAATCCGGTAAATTCAACCCGGAAATCCTGGAAAAAGAGAACGCTTATCTGGTG 
                   
               
               
                   
                 GGTTATTGGCAGTGCGACAAGTACTTCGACGACAAAGATGTGGTTCGCGAAATTCGTGAA 
                   
               
               
                   
                 GCGTTCGAGAAAAAACCGCAGGAGCTGATGACCGACGCCAGCTCTTGGTCTACTCTACAG 
                   
               
               
                   
                 CAGATTGAATGCTGCGAGTCCGTATCCCTGCACGTACGTCGTACTGATTACGTGGACGAG 
                   
               
               
                   
                 GAACATATTCATATCCATAACATCTGTACGGAAAAATACTATAAAAACGCCATTGATCGT 
                   
               
               
                   
                 GTGCGTAAACAGTACCCGAGCGCAGTGTTCTTCATCTTCACCGATGATAAAGAATGGTGC 
                   
               
               
                   
                 CGCGACCACTTTAAAGGTCCGAACTTCATCGTAGTCGAACTGGAAGAAGGCGACGGTACC 
                   
               
               
                   
                 GACATCGCTGAAATGACTCTGATGTCCCGCTGTAAACATCACATCATCGCTAATTCTAGC 
                   
               
               
                   
                 TTTAGCTGGTGGGCGGCGTGGCTGAACGACTCCCCGGAAAAAATCGTGATCGCTCCTCAG 
                   
               
               
                   
                 AAATGGATTAACAACCGCGACATGGACGATATTTACACCGAGCGTATGACTAAAATCGCA 
                   
               
               
                   
                 CTGTAGCTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutW 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG CGCCTGGTTAAAATGATCGGCGGT 
                 283 
               
               
                   
                 CTGGGTAATCAGATGTTCATCTACGCGTTTTACCTACAGATGCGTAAGCGTTTCTCCAAC 
                   
               
               
                   
                 GTTCGTATCGACCTGACCGATATGATGCACTACAACGTACACTATGGCTACGAACTGCAC 
                   
               
               
                   
                 AAAGTTTTCGGTCTGCCGCGCACCGAGTTCTGTATGAACCAGCCTCTGAAAAAGGTTCTG 
                   
               
               
                   
                 GAGTTCCTGTTCTTCCGTACCATTGTTGAACGTAAACAGCACGGTCGTATGGAGCCGTAT 
                   
               
               
                   
                 ACTTGCCAGTATGTTTGGCCGCTGGTTTACTTTAAGGGCTTCTATCAGTCCGAACGTTAC 
                   
               
               
                   
                 TTCTCCGAAGTTAAGGACGAAGTTCGTGAGTGTTTCACCTTCAATCCGGCACTGGCGAAT 
                   
               
               
                   
                 CGTTCTTCCCAACAGATGATGGAACAGATCCAGAATGATCCTCAGGCTGTCTCTATCCAC 
                   
               
               
                   
                 ATCCGTCGTGGCGACTATCTGAATCCGAAGCACTACGACACTATCGGTTGTATCTGTCAG 
                   
               
               
                   
                 CTGCCGTATTACAAGCACGCCGTTTCCGAAATTAAAAAGTACGTTTCTAACCCTCACTTT 
                   
               
               
                   
                 TACGTTTTCTCCGAAGACCTGGATTGGGTCAAAGCAAACCTGCCGCTGGAAAACGCACAG 
                   
               
               
                   
                 TACATCGATTGGAACAAAGGCGCAGATAGCTGGCAGGATATGATGCTGATGAGCTGTTGC 
                   
               
               
                   
                 AAACACCACATTATCTGTAACTCCACCTTTAGCTGGTGGGCGGCGTGGCTGAACCCATCT 
                   
               
               
                   
                 GTCGAAAAAACCGTGATCATGCCGGAACAGTGGACGTCTCGTCAAGATTCCGTGGACTTT 
                   
               
               
                   
                 GTGGCTAGCTGTGGCCGTTGGGTCCGTGTTAAAACGGAGTAGCTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutX 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG CGTCTGATCAAGATGATCGGCGGC 
                 284 
               
               
                   
                 CTGGGTAACCAGATGTTTATCTACGCGTTCTACCTGAAAATGAAACACCATTACCCGGAT 
                   
               
               
                   
                 ACGAACATCGATCTGTCTGACATGGTTCATTATAAAGTTCACAACGGTTATGAGATGAAC 
                   
               
               
                   
                 CGTATCTTTGACCTGAGCCAGACTGAATTTTGCATCAACCGTACCCTGAAAAAAATCCTG 
                   
               
               
                   
                 GAGTTCCTGTTCTTCAAAAAAATCTACGAACGTCGCCAGGACCCGTCTACTCTGTATCCA 
                   
               
               
                   
                 TACGAAAAACGTTATTTTTGGCCGCTGCTGTACTTTAAAGGTTTCTACCAGTCTGAACGC 
                   
               
               
                   
                 TTCTTCTTCGATATCAAAGACGACGTTCGTAAAGCCTTCTCTTTTAACCTGAACATCGCT 
                   
               
               
                   
                 AACCCGGAAAGCCTGGAACTGCTGAAACAGATCGAAGTTGACGACCAAGCTGTTTCTATC 
                   
               
               
                   
                 CACATCCGCCGTGGTGACTACCTGCTGCCGCGTCACTGGGCAAACACGGGTTCCGTGTGC 
                   
               
               
                   
                 CAGCTGCCGTATTACAAGAACGCGATCGCGGAAATGGAGAACCGTATTACTGGCCCGAGC 
                   
               
               
                   
                 TACTACGTGTTCTCTGATGATATCTCTTGGGTTAAAGAAAACATCCCGCTGAAGAAAGCG 
                   
               
               
                   
                 GTCTACGTGACGTGGAACAAGGGCGAAGACAGCTGGCAGGATATGATGCTGATGAGCCAC 
                   
               
               
                   
                 TGTCGTCACCACATTATCTGTAATTCTACGTTCTCCTGGTGGGGTGCTTGGCTGAACCCA 
                   
               
               
                   
                 CGTAAAGAGAAAATCGTCATCGCGCCGTGTCGCTGGTTCCAGCATAAAGAAACCCCGGAC 
                   
               
               
                   
                 ATGTACCCGAAAGAATGGATCAAAGTACCGATTAACTAGCTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutZ 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG TATTCTTGCCTGTCTGGTGGCCTG 
                 285 
               
               
                   
                 GGTAACCAAATGTTTCAATACGCAGCAGCGTATATCCTGAAGCAGTATTTTCAGTCTACC 
                   
               
               
                   
                 ACTCTGGTCCTGGATGATAGCTATTACTATTCCCAGCCGAAACGTGATACCGTTCGTAGC 
                   
               
               
                   
                 CTGGAACTGAATCAGTTCAACATCTCTTATGATCGTTTTAGCTTCGCGGATGAAAAAGAG 
                   
               
               
                   
                 AAGATCAAACTGCTGCGCAAATTCAAACGTAACCCGTTCCCTAAACAGATTTCCGAGATC 
                   
               
               
                   
                 CTGTCTATTGCGCTGTTCGGCAAATACGCGCTGTCCGACCGTGCATTTTACACCTTCGAA 
                   
               
               
                   
                 ACTATCAAAAACATCGACAAAGCGTGCCTGTTCTCCTTTTACCAGGACGCCGATCTGCTG 
                   
               
               
                   
                 AATAAATATAAGCAGCTGATCCTGCCGCTGTTCGAACTGCGCGATGACCTGCTGGATATC 
                   
               
               
                   
                 TGCAAGAACCTGGAACTGTATTCCCTGATCCAACGCAGCAACAATACCACTGCACTGCAT 
                   
               
               
                   
                 ATCCGCCGTGGCGACTACGTGACCAACCAGCACGCCGCGAAATACCACGGCGTGCTGGAC 
                   
               
               
                   
                 ATCAGCTACTATAACCACGCAATGGAATACGTGGAACGTGAACGCGGCAAACAGAACTTC 
                   
               
               
                   
                 ATTATCTTCAGCGATGATGTACGTTGGGCACAGAAAGCGTTTCTGGAGAACGATAATTGC 
                   
               
               
                   
                 TACGTGATTAACAACTCCGACTACGATTTCTCTGCGATCGATATGTATCTGATGTCTCTG 
                   
               
               
                   
                 TGCAAAAACAACATCATCGCAAATTCCACCTACTCCTGGTGGGGTGCGTGGCTGAACAAA 
                   
               
               
                   
                 TACGAGGACAAACTGGTTATCTCTCCGAAACAATGGTTTCTGGGTAACAACGAAACCTCT 
                   
               
               
                   
                 CTGCGTAACGCGTCTTGGATCACCCTGTAGCTCGAGTGACTGACTG 
                   
               
               
                   
               
               
                 FutZA 
                 CAGTCAGTCAGAATTCAAGAAGGAGATATACAT ATG CGTCTGATCAAGATGACCGGTGGC 
                 286 
               
               
                   
                 CTGGGTAACCAGATGTTCATCTACGCGTTTTATCTGCGTATGAAAAAACGTTATCCGAAA 
                   
               
               
                   
                 GTTCGTATTGATCTGTCTGATATGGTTCATTATCACGTTCACCACGGCTATGAAATGCAC 
                   
               
               
                   
                 CGTGTTTTCAATCTGCCGCACACCGAATTTTGCATCAACCAGCCGCTGAAAAAAGTGATC 
                   
               
               
                   
                 GAGTTCCTGTTTTTCAAAAAGATTTACGAACGTAAACAGGACCCTAATTCTCTGCGTGCA 
                   
               
               
                   
                 TTCGAGAAGAAGTATCTGTGGCCGCTGCTGTACTTCAAAGGTTTCTATCAATCTGAGCGC 
                   
               
               
                   
                 TTCTTTGCTGACATCAAAGACGAGGTTCGTAAAGCATTCACCTTTGACTCTTCTAAAGTG 
                   
               
               
                   
                 AACGCTCGCTCTGCCGAACTGCTGCGTCGCCTGGATGCCGATGCTAACGCGGTTAGCCTG 
                   
               
               
                   
                 CACATTCGTCGCGGTGACTATCTACAGCCGCAGCATTGGGCTACCACTGGTTCTGTCTGC 
                   
               
               
                   
                 CAGCTGCCGTACTACCAGAACGCGATCGCTGAAATGAACCGTCGCGTTGCTGCCCCGAGC 
                   
               
               
                   
                 TACTACGTTTTCAGCGATGACATCGCGTGGGTGAAGGAAAACATCCCTCTACAGAACGCA 
                   
               
               
                   
                 GTGTACATCGACTGGAATAAAGGCGAAGAAAGCTGGCAGGATATGATGCTGATGAGCCAC 
                   
               
               
                   
                 TGCCGCCACCACATTATCTGTAACAGCACCTTCTCTTGGTGGGGCGCGTGGCTGGACCCG 
                   
               
               
                   
                 CACGAGGACAAAATTGTAATCGTTCCGAATCGTTGGTTCCAGCATTGCGAAACTCCTAAC 
                   
               
               
                   
                 ATCTATCCGGCAGGCTGGGTGAAAGTTGCGATTAATTAGCTCGAGTGACTGACTG 
               
               
                   
               
            
           
         
       
     
     In any of the methods described herein, the α(1,2) fucosyltransferase genes or gene products may be variants or functional fragments thereof. A variant of any of genes or gene products disclosed herein may have 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity to the nucleic acid or amino acid sequences described herein. 
     Variants as disclosed herein also include homolog, orthologs, or paralogs of the genes or gene products described herein that retain the same biological function as the genes or gene products specified herein. These variants can be used interchangeably with the genes recited in these methods. Such variants may demonstrate a percentage of homology or identity, for example, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity conserved domains important for biological function, preferably in a functional domain, e.g. catalytic domain. 
     The term “% identity,” in the context of two or more nucleic acid or polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same, when compared and aligned for maximum correspondence, as measured using one of the following sequence comparison algorithms or by visual inspection. For example, % identity is relative to the entire length of the coding regions of the sequences being compared, or the length of a particular fragment or functional domain thereof. 
     For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. 
     Percent identity is determined using search algorithms such as BLAST and PSI-BLAST (Altschul et al., 1990, J Mol Biol 215:3, 403-410; Altschul et al., 1997, Nucleic Acids Res 25:17, 3389-402). For the PSI-BLAST search, the following exemplary parameters are employed: (1) Expect threshold was 10; (2) Gap cost was Existence:11 and Extension:1; (3) The Matrix employed was BLOSUM62; (4) The filter for low complexity regions was “on”. 
     Changes can be introduced by mutation into the nucleic acid sequence or amino acid sequence of any of the genes or gene products described herein, leading to changes in the amino acid sequence of the encoded protein or enzyme, without altering the functional ability of the protein or enzyme. For example, nucleotide substitutions leading to amino acid substitutions at “non-essential” amino acid residues can be made in the sequence of any of sequences expressly disclosed herein. A “non-essential” amino acid residue is a residue at a position in the sequence that can be altered from the wild-type sequence of the polypeptide without altering the biological activity, whereas an “essential” amino acid residue is a residue at a position that is required for biological activity. For example, amino acid residues that are conserved among members of a family of proteins are not likely to be amenable to mutation. Other amino acid residues, however, (e.g., those that are poorly conserved among members of the protein family) may not be as essential for activity and thus are more likely to be amenable to alteration. Thus, another aspect of the invention pertains to nucleic acid molecules encoding the proteins or enzymes disclosed herein that contain changes in amino acid residues relative to the amino acid sequences disclosed herein that are not essential for activity (i.e., fucosyltransferase activity). 
     An isolated nucleic acid molecule encoding a protein essentially retaining the functional capability compared to any of the genes described herein can be created by introducing one or more nucleotide substitutions, additions or deletions into the corresponding nucleotide sequence, such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein. 
     Mutations can be introduced into a nucleic acid sequence by standard techniques such that the encoded amino acid sequence is altered, such as site-directed mutagenesis and PCR-mediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A “conservative amino acid substitution” is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. Certain amino acids have side chains with more than one classifiable characteristic. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, tryptophan, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tyrosine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in a given polypeptide is replaced with another amino acid residue from the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly along all or part of a given coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for given polypeptide biological activity to identify mutants that retain activity. Conversely, the invention also provides for variants with mutations that enhance or increase the endogenous biological activity. Following mutagenesis of the nucleic acid sequence, the encoded protein can be expressed by any recombinant technology known in the art and the activity of the protein can be determined. An increase, decrease, or elimination of a given biological activity of the variants disclosed herein can be readily measured by the ordinary person skilled in the art, i.e., by measuring the capability for mediating oligosaccharide modification, synthesis, or degradation (via detection of the products). 
     The present invention also provides for functional fragments of the genes or gene products described herein. A fragment, in the case of these sequences and all others provided herein, is defined as a part of the whole that is less than the whole. Moreover, a fragment ranges in size from a single nucleotide or amino acid within a polynucleotide or polypeptide sequence to one fewer nucleotide or amino acid than the entire polynucleotide or polypeptide sequence. Finally, a fragment is defined as any portion of a complete polynucleotide or polypeptide sequence that is intermediate between the extremes defined above. 
     For example, fragments of any of the proteins or enzymes disclosed herein or encoded by any of the genes disclosed herein can be 10 to 20 amino acids, 10 to 30 amino acids, 10 to 40 amino acids, 10 to 50 amino acids, 10 to 60 amino acids, 10 to 70 amino acids, 10 to 80 amino acids, 10 to 90 amino acids, 10 to 100 amino acids, 50 to 100 amino acids, 75 to 125 amino acids, 100 to 150 amino acids, 150 to 200 amino acids, 200 to 250 amino acids, 250 to 300 amino acids, 300 to 350 amino acids, 350 to 400 amino acids, 400 to 450 amino acids, or 450 to 500 amino acids. The fragments encompassed in the present invention comprise fragments that retain functional fragments. As such, the fragments preferably retain the catalytic domains that are required or are important for functional activity. Fragments can be determined or generated by using the sequence information herein, and the fragments can be tested for functional activity using standard methods known in the art. For example, the encoded protein can be expressed by any recombinant technology known in the art and the activity of the protein can be determined. The biological function of said fragment can be measured by measuring ability to synthesize or modify a substrate oligosaccharide, or conversely, to catabolize an oligosaccharide substrate. 
     Within the context of the invention, “functionally equivalent”, as used herein, refers to a gene or the resulting encoded protein variant or fragment thereof capable of exhibiting a substantially similar activity as the wild-type fucosyltransferase. Specifically, the fucosyltransferase activity refers to the ability to transfer a fucose sugar to an acceptor substrate via an alpha-(1,2)-linkage. As used herein, “substantially similar activity” refers to an activity level within 5%, 10%, 20%, 30%, 40%, or 50% of the wild-type fucosyltransferase. 
     To test for lactose-utilizing fucosyltransferase activity, the production of fucosylated oligosaccharides (i.e., 2′-FL) is evaluated in a host organism that expresses the candidate enzyme (or syngene) and which contains both cytoplasmic GDP-fucose and lactose pools. The production of fucosylated oligosaccharides indicates that the candidate enzyme-encoding sequence functions as a lactose-utilizing α(1,2)fucosyltransferase. 
     Engineering of  E. coli  to Produce Human Milk Oligosaccharide 2′-FL 
     Described herein is a gene screening approach, which was used to validate the novel α (1,2) fucosyltransferases (α (1,2) FTs) for the synthesis of fucosyl-linked oligosaccharides in metabolically engineered  E. coli . Of particular interest are α (1,2) FTs that are capable of the synthesis of the HMOS 2′-fucosyllactose (2′-FL). 2′-FL is the most abundant fucosylated oligosaccharide present in human milk, and this oligosaccharide provides protection to newborn infants against infectious diarrhea caused by bacterial pathogens such as  Campylobacter jejuni  (Ruiz-Palacios, G. M., et al. (2003). J Biol Chem 278, 14112-120; Morrow, A. L. et al. (2004). J Pediatr 145, 297-303; Newburg, D. S. et al. (2004). Glycobiology 14, 253-263). Other α (1,2) FTs of interest are those capable of synthesis of HMOS lactodifucotetraose (LDFT), laco-N-fucopentaose I (LNFI), or lacto-N-difucohexaose I (LDFH I). 
     The synthetic pathway of fucosyl oligosaccharides of human milk is illustrated in  FIG.  1   . Structurally, 2′-FL consists of a fucose molecule a 1,2 linked to the galactose portion of lactose (Fucα1-2Galβ1-4G1c). An α (1,2) FT from  H. pylori  strain 26695 termed FutC has been utilized to catalyze the synthesis of 2′-FL in metabolically engineered  E. coli  (Drouillard, S. et al. (2006). Angew Chem Int Ed Engl 45, 1778-780). 
     Candidate α(1,2) FTs (i.e., syngenes) were cloned by standard molecular biological techniques into an expression plasmid. This plasmid utilizes the strong leftwards promoter of bacteriophage λ (termed P L ) to direct expression of the candidate genes (Sanger, F. et al. (1982). J Mol Biol 162, 729-773). The promoter is controllable, e.g., a trp-cI construct is stably integrated the into the  E. coli  host&#39;s genome (at the ampC locus), and control is implemented by adding tryptophan to the growth media. Gradual induction of protein expression is accomplished using a temperature sensitive cI repressor. Another similar control strategy (temperature independent expression system) has been described (Mieschendahl et al., 1986, Bio/Technology 4:802-808). The plasmid also carries the  E. coli  rcsA gene to up-regulate GDP-fucose synthesis, a critical precursor for the synthesis of fucosyl-linked oligosaccharides. In addition, the plasmid carries a β-lactamase (bla) gene for maintaining the plasmid in host strains by ampicillin selection (for convenience in the laboratory) and a native thyA (thymidylate synthase) gene as an alternative means of selection in thyA −  hosts. Alternative selectable markers include the proBA genes to complement proline auxotrophy (Stein et al., (1984), J Bacteriol 158:2, 696-700 (1984) or  purA  to complement adenine auxotrophy (S. A. Wolfe, J. M. Smith,  J Biol Chem  263, 19147-53 (1988)). To act as plasmid selectable markers each of these genes are first inactivated in the host cell chromosome, then wild type copies of the genes are provided on the plasmid. Alternatively a drug resistance gene may be used on the plasmid, e.g. beta-lactamase (this gene is already on the expression plasmid described above, thereby permitting selection with ampicillin). Ampicillin selection is well known in the art and described in standard manuals such as Maniatis et al., (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring, N.Y. 
     The nucleic acid sequence of such an expression plasmid, pEC2-(T7)FutX-rcsA-thyA (pG401) is provided below. The underlined sequence represents the FutX syngene, which can be readily replaced with any of the novel α(1,2) FTs described herein using standard recombinant DNA techniques. 
     
       
         
           
               
               
            
               
                 (SEQ ID NO: 287) 
                   
               
               
                 TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGT 
                   
               
               
                   
               
               
                 CTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGG 
               
               
                   
               
               
                 CTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACC 
               
               
                   
               
               
                 GCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCTCCTCAACCTGTATATTCGTAAACCACGCC 
               
               
                   
               
               
                 CAATGGGAGCTGTCTCAGGTTTGTTCCTGATTGGTTACGGCGCGTTTCGCATCATTGTTGAGTTTTTC 
               
               
                   
               
               
                 CGCCAGCCCGACGCGCAGTTTACCGGTGCCTGGGTGCAGTACATCAGCATGGGGCAAATTCTTTCCAT 
               
               
                   
               
               
                 CCCGATGATTGTCGCGGGTGTGATCATGATGGTCTGGGCATATCGTCGCAGCCCACAGCAACACGTTT 
               
               
                   
               
               
                 CCTGAGGAACCATGAAACAGTATTTAGAACTGATGCAAAAAGTGCTCGACGAAGGCACACAGAAAAAC 
               
               
                   
               
               
                 GACCGTACCGGAACCGGAACGCTTTCCATTTTTGGTCATCAGATGCGTTTTAACCTGCAAGATGGATT 
               
               
                   
               
               
                 CCCGCTGGTGACAACTAAACGTTGCCACCTGCGTTCCATCATCCATGAACTGCTGTGGTTTCTGCAGG 
               
               
                   
               
               
                 GCGACACTAACATTGCTTATCTACACGAAAACAATGTCACCATCTGGGACGAATGGGCCGATGAAAAC 
               
               
                   
               
               
                 GGCGACCTCGGGCCAGTGTATGGTAAACAGTGGCGCGCCTGGCCAACGCCAGATGGTCGTCATATTGA 
               
               
                   
               
               
                 CCAGATCACTACGGTACTGAACCAGCTGAAAAACGACCCGGATTCGCGCCGCATTATTGTTTCAGCGT 
               
               
                   
               
               
                 GGAACGTAGGCGAACTGGATAAAATGGCGCTGGCACCGTGCCATGCATTCTTCCAGTTCTATGTGGCA 
               
               
                   
               
               
                 GACGGCAAACTCTCTTGCCAGCTTTATCAGCGCTCCTGTGACGTCTTCCTCGGCCTGCCGTTCAACAT 
               
               
                   
               
               
                 TGCCAGCTACGCGTTATTGGTGCATATGATGGCGCAGCAGTGCGATCTGGAAGTGGGTGATTTTGTCT 
               
               
                   
               
               
                 GGACCGGTGGCGACACGCATCTGTACAGCAACCATATGGATCAAACTCATCTGCAATTAAGCCGCGAA 
               
               
                   
               
               
                 CCGCGTCCGCTGCCGAAGTTGATTATCAAACGTAAACCCGAATCCATCTTCGACTACCGTTTCGAAGA 
               
               
                   
               
               
                 CTTTGAGATTGAAGGCTACGATCCGCATCCGGGCATTAAAGCGCCGGTGGCTATCTAATTACGAAACA 
               
               
                   
               
               
                 TCCTGCCAGAGCCGACGCCAGTGTGCGTCGGTTTTTTTACCCTCCGTTAAATTCTTCGAGACGCCTTC 
               
               
                   
               
               
                 CCGAAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGC 
               
               
                   
               
               
                 TATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCC 
               
               
                   
               
               
                 CAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTTCTTTAATGAAGCAGGGCATCAGGACGGT 
               
               
                   
               
               
                 ATCTTTGTGGAGAAAGCAGAGTAATCTTATTCAGCCTGACTGGTGGGAAACCACCAGTCAGAATGTGT 
               
               
                   
               
               
                 TAGCGCATGTTGACAAAAATACCATTAGTCACATTATCCGTCAGTCGGACGACATGGTAGATAACCTG 
               
               
                   
               
               
                 TTTATTATGCGTTTTGATCTTACGTTTAATATTACCTTTATGCGATGAAACGGTCTTGGCTTTGATAT 
               
               
                   
               
               
                 TCATTTGGTCAGAGATTTGAATGGTTCCCTGACCTGCCATCCACATTCGCAACATACTCGATTCGGTT 
               
               
                   
               
               
                 CGGCTCAATGATAACGTCGGCATATTTAAAAACGAGGTTATCGTTGTCTCTTTTTTCAGAATATCGCC 
               
               
                   
               
               
                 AAGGATATCGTCGAGAGATTCCGGTTTAATCGATTTAGAACTGATCAATAAATTTTTTCTGACCAATA 
               
               
                   
               
               
                 GATATTCATCAAAATGAACATTGGCAATTGCCATAAAAACGATAAATAACGTATTGGGATGTTGATTA 
               
               
                   
               
               
                 ATGATGAGCTTGATACGCTGACTGTTAGAAGCATCGTGGATGAAACAGTCCTCATTAATAAACACCAC 
               
               
                   
               
               
                 TGAAGGGCGCTGTGAATCACAAGCTATGGCAAGGTCATCAACGGTTTCAATGTCGTTGATTTCTCTTT 
               
               
                   
               
               
                 TTTTAACCCCTCTACTCAACAGATACCCGGTTAAACCTAGTCGGGTGTAACTACATAAATCCATAATA 
               
               
                   
               
               
                 ATCGTTGACATGGCATACCCTCACTCAATGCGTAACGATAATTCCCCTTACCTGAATATTTCATCATG 
               
               
                   
               
               
                 ACTAAACGGAACAACATGGGTCACCTAATGCGCCACTCTCGCGATTTTTCAGGCGGACTTACTATCCC 
               
               
                   
               
               
                 GTAAAGTGTTGTATAATTTGCCTGGAATTGTCTTAAAGTAAAGTAAATGTTGCGATATGTGAGTGAGC 
               
               
                   
               
               
                 TTAAAACAAATATTTCGCTGCAGGAGTATCCTGGAAGATGTTCGTAGAAGCTTACTGCTCACAAGAAA 
               
               
                   
               
               
                 AAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGCAGCTCGAG CTAG   
               
               
                   
               
               
                 
                   TTAATCGGTACTTTGATCCATTCTTTCGGGTACATGTCCGGGGTTTCTTTATGCTGGAACCAGCGACA 
                 
               
               
                   
               
               
                 
                   CGGCGCGATGACGATTTTCTCTTTACGTGGGTTCAGCCAAGCACCCCACCAGGAGAACGTAGAATTAC 
                 
               
               
                   
               
               
                 
                   AGATAATGTGGTGACGACAGTGGCTCATCAGCATCATATCCTGCCAGCTGTCTTCGCCCTTGTTCCAC 
                 
               
               
                   
               
               
                 
                   GTCACGTAGACCGCTTTCTTCAGCGGGATGTTTTCTTTAACCCAAGAGATATCATCAGAGAACACGTA 
                 
               
               
                   
               
               
                 
                   GTAGCTCGGGCCAGTAATACGGTTCTCCATTTCCGCGATCGCGTTCTTGTAATACGGCAGCTGGCACA 
                 
               
               
                   
               
               
                 
                   CGGAACCCGTGTTTGCCCAGTGACGCGGCAGCAGGTAGTCACCACGGCGGATGTGGATAGAAACAGCT 
                 
               
               
                   
               
               
                 
                   TGGTCGTCAACTTCGATCTGTTTCAGCAGTTCCAGGCTTTCCGGGTTAGCGATGTTCAGGTTAAAAGA 
                 
               
               
                   
               
               
                 
                   GAAGGCTTTACGAACGTCGTCTTTGATATCGAAGAAGAAGCGTTCAGACTGGTAGAAACCTTTAAAGT 
                 
               
               
                   
               
               
                 
                   ACAGCAGCGGCCAAAAATAACGTTTTTCGTATGGATACAGAGTAGACGGGTCCTGGCGACGTTCGTAG 
                 
               
               
                   
               
               
                 
                   ATTTTTTTGAAGAACAGGAACTCCAGGATTTTTTTCAGGGTACGGTTGATGCAAAATTCAGTCTGGCT 
                 
               
               
                   
               
               
                 
                   CAGGTCAAAGATACGGTTCATCTCATAACCGTTGTGAACTTTATAATGAACCATGTCAGACAGATCGA 
                 
               
               
                   
               
               
                 
                   TGTTCGTATCCGGGTAATGGTGTTTCATTTTCAGGTAGAACGCGTAGATAAACATCTGGTTACCCAGG 
                 
               
               
                   
               
               
                   CCGCCGATCATCTTGATCAGACGCAT ATGTATATCTCCTTCTTGAATTCTAAAAATTGATTGAATGTA 
               
               
                   
               
               
                 TGCAAATAAATGCATACACCATAGGTGTGGTTTAATTTGATGCCCTTTTTCAGGGCTGGAATGTGTAA 
               
               
                   
               
               
                 GAGCGGGGTTATTTATGCTGTTGTTTTTTTGTTACTCGGGAAGGGCTTTACCTCTTCCGCATAAACGC 
               
               
                   
               
               
                 TTCCATCAGCGTTTATAGTTAAAAAAATCTTTCGGAACTGGTTTTGCGCTTACCCCAACCAACAGGGG 
               
               
                   
               
               
                 ATTTGCTGCTTTCCATTGAGCCTGTTTCTCTGCGCGACGTTCGCGGCGGCGTGTTTGTGCATCCATCT 
               
               
                   
               
               
                 GGATTCTCCTGTCAGTTAGCTTTGGTGGTGTGTGGCAGTTGTAGTCCTGAACGAAAACCCCCCGCGAT 
               
               
                   
               
               
                 TGGCACATTGGCAGCTAATCCGGAATCGCACTTACGGCCAATGCTTCGTTTCGTATCACACACCCCAA 
               
               
                   
               
               
                 AGCCTTCTGCTTTGAATGCTGCCCTTCTTCAGGGCTTAATTTTTAAGAGCGTCACCTTCATGGTGGTC 
               
               
                   
               
               
                 AGTGCGTCCTGCTGATGTGCTCAGTATCACCGCCAGTGGTATTTATGTCAACACCGCCAGAGATAATT 
               
               
                   
               
               
                 TATCACCGCAGATGGTTATCTGTATGTTTTTTATATGAATTTATTTTTTGCAGGGGGGCATTGTTTGG 
               
               
                   
               
               
                 TAGGTGAGAGATCAATTCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGG 
               
               
                   
               
               
                 CGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGC 
               
               
                   
               
               
                 TCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA 
               
               
                   
               
               
                 AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCC 
               
               
                   
               
               
                 CCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATA 
               
               
                   
               
               
                 CCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC 
               
               
                   
               
               
                 TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCG 
               
               
                   
               
               
                 GTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT 
               
               
                   
               
               
                 ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG 
               
               
                   
               
               
                 GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTAC 
               
               
                   
               
               
                 GGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT 
               
               
                   
               
               
                 TGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA 
               
               
                   
               
               
                 TTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGG 
               
               
                   
               
               
                 AACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTT 
               
               
                   
               
               
                 AAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAAT 
               
               
                   
               
               
                 GCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCC 
               
               
                   
               
               
                 GTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGA 
               
               
                   
               
               
                 CCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTG 
               
               
                   
               
               
                 GTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCG 
               
               
                   
               
               
                 CCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGG 
               
               
                   
               
               
                 TATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAA 
               
               
                   
               
               
                 AAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATG 
               
               
                   
               
               
                 GTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA 
               
               
                   
               
               
                 GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATAC 
               
               
                   
               
               
                 GGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGA 
               
               
                   
               
               
                 AAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATC 
               
               
                   
               
               
                 TTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAA 
               
               
                   
               
               
                 AGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATT 
               
               
                   
               
               
                 TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGT 
               
               
                   
               
               
                 TCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCT 
               
               
                   
               
               
                 ATAAAAATAGGCGTATCACGAGGCCCTTTCGTC 
               
            
           
         
       
     
     The expression constructs were transformed into a host strain useful for the production of 2′-FL. Biosynthesis of 2′-FL requires the generation of an enhanced cellular pool of both lactose and GDP-fucose ( FIG.  2   ). The wild-type  Escherichia coli  K12 prototrophic strain W3110 was selected as the parent background to test the ability of the candidates to catalyze 2′-FL production (Bachmann, B J (1972). Bacteriol Rev 36, 525-557). The particular W3110 derivative employed was one that previously had been modified by the introduction (at the ampC locus) of a tryptophan-inducible P trpB  cI+ repressor cassette, generating an  E. coli  strain known as GI724 (LaVallie, E. R. et al. (2000). Methods Enzymol 326, 322-340). Other features of GI724 include lacIq and lacPL8 promoter mutations.  E. coli  strain GI724 affords economical production of recombinant proteins from the phage λ P L  promoter following induction with low levels of exogenous tryptophan (LaVallie, E. R. et al. (1993). Biotechnology (N Y) 11, 187-193; Mieschendahl, et al. (1986). Bio/Technology 4, 802-08). Additional genetic alterations were made to this strain to promote the biosynthesis of 2′-FL. This was achieved in strain GI724 through several manipulations of the chromosome using λ Red recombineering (Court, D. L. et al. (2002). Annu Rev Genet 36, 361-388) and generalized P1 phage transduction. 
     First, the ability of the  E. coli  host strain to accumulate intracellular lactose was engineered by simultaneous deletion of the endogenous β-galactosidase gene (lacZ) and the lactose operon repressor gene (lad). During construction of this deletion, the lacIq promoter was placed immediately upstream of the lactose permease gene, lacY. The modified strain maintains its ability to transport lactose from the culture medium (via LacY), but is deleted for the wild-type copy of the lacZ (β-galactosidase) gene responsible for lactose catabolism. Therefore, an intracellular lactose pool is created when the modified strain is cultured in the presence of exogenous lactose. A schematic of the P lacIq  lacY +  chromosomal construct is shown in  FIG.  12   . 
     Genomic DNA sequence of the P lacIq  lacY +  chromosomal construct is set forth below (SEQ ID NO: 288): 
     
       
         
           
               
               
            
               
                 CACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAAGTGTAGGCTGGAGC 
                   
               
               
                   
               
               
                 TGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCGGAATAGGAACTAAGGAGGAT 
               
               
                   
               
               
                 ATTCATATGTACTATTTAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTTTTTATCATG 
               
               
                   
               
               
                 GGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATT 
               
               
                   
               
               
                 TTTGCCGCTATTTCTCTGTTCTCGCTATTATTCCAACCGCTGTTTGGTCTGCTTTCTGACAAACTCGGGCTGCGC 
               
               
                   
               
               
                 AAATACCTGCTGTGGATTATTACCGGCATGTTAGTGATGTTTGCGCCGTTCTTTATTTTTATCTTCGGGCCACTG 
               
               
                   
               
               
                 TTACAATACAACATTTTAGTAGGATCGATTGTTGGTGGTATTTATCTAGGCTTTTGTTTTAACGCCGGTGCGCCA 
               
               
                   
               
               
                 GCAGTAGAGGCATTTATTGAGAAAGTCAGCCGTCGCAGTAATTTCGAATTTGGTCGCGCGCGGATGTTTGGCTGT 
               
               
                   
               
               
                 GTTGGCTGGGCGCTGTGTGCCTCGATTGTCGGCATCATGTTCACCATCAATAATCAGTTTGTTTTCTGGCTGGGC 
               
               
                   
               
               
                 TCTGGCTGTGCACTCATCCTCGCCGTTTTACTCTTTTTCGCCAAAACGGATGCGCCCTCTTCTGCCACGGTTGCC 
               
               
                   
               
               
                 AATGCGGTAGGTGCCAACCATTCGGCATTTAGCCTTAAGCTGGCACTGGAACTGTTCAGACAGCCAAAACTGTGG 
               
               
                   
               
               
                 TTTTTGTCACTGTATGTTATTGGCGTTTCCTGCACCTACGATGTTTTTGACCAACAGTTTGCTAATTTCTTTACT 
               
               
                   
               
               
                 TCGTTCTTTGCTACCGGTGAACAGGGTACGCGGGTATTTGGCTACGTAACGACAATGGGCGAATTACTTAACGCC 
               
               
                   
               
               
                 TCGATTATGTTCTTTGCGCCACTGATCATTAATCGCATCGGTGGGAAAAACGCCCTGCTGCTGGCTGGCACTATT 
               
               
                   
               
               
                 ATGTCTGTACGTATTATTGGCTCATCGTTCGCCACCTCAGCGCTGGAAGTGGTTATTCTGAAAACGCTGCATATG 
               
               
                   
               
               
                 TTTGAAGTACCGTTCCTGCTGGTGGGCTGCTTTAAATATATTACCAGCCAGTTTGAAGTGCGTTTTTCAGCGACG 
               
               
                   
               
               
                 ATTTATCTGGTCTGTTTCTGCTTCTTTAAGCAACTGGCGATGATTTTTATGTCTGTACTGGCGGGCAATATGTAT 
               
               
                   
               
               
                 GAAAGCATCGGTTTCCAGGGCGCTTATCTGGTGCTGGGTCTGGTGGCGCTGGGCTTCACCTTAATTTCCGTGTTC 
               
               
                   
               
               
                 ACGCTTAGCGGCCCCGGCCCGCTTTCCCTGCTGCGTCGTCAGGTGAATGAAGTCGCTTAAGCAATCAATGTCGGA 
               
               
                   
               
               
                 TGCGGCGCGAGCGCCTTATCCGACCAACATATCATAACGGAGTGATCGCATTGTAAATTATAAAAATTGCCTGAT 
               
               
                   
               
               
                 ACGCTGCGCTTATCAGGCCTACAAGTTCAGCGATCTACATTAGCCGCATCCGGCATGAACAAAGCGCAGGAACAA 
               
               
                   
               
               
                 GCGTCGCA 
               
            
           
         
       
     
     Second, the ability of the host  E. coli  strain to synthesize colanic acid, an extracellular capsular polysaccharide, was eliminated by the deletion of the wcaJ gene, encoding the UDP-glucose lipid carrier transferase (Stevenson, G. et al. (1996). J Bacteriol 178, 4885-893). In a wcaJ null background GDP-fucose accumulates in the  E. coli  cytoplasm (Dumon, C. et al. (2001). Glycoconj J 18, 465-474). A schematic of the chromosomal deletion of wcaJ is shown in  FIG.  13   . 
     The sequence of the chromosomal region of  E. coli  bearing the ΔwcaJ::FRT mutation is set forth below (SEQ ID NO: 289): 
     
       
         
           
               
               
            
               
                 GTTCGGTTATATCAATGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCC 
                   
               
               
                   
               
               
                 GGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCGCCCGTGGAATTAATCAAAGTGCACAACACGCC 
               
               
                   
               
               
                 GGACGGCAATTTCCCCAACGGTATTCCTAACCCACTACTGCCGGAATGCCGCGACGACACCCGCAATGCGGTCAT 
               
               
                   
               
               
                 CAAACACGGCGCGGATATGGGCATTGCTTTTGATGGCGATTTTGACCGCTGTTTCCTGTTTGACGAAAAAGGGCA 
               
               
                   
               
               
                 GTTTATTGAGGGCTACTACATTGTCGGCCTGTTGGCAGAAGCATTCCTCGAAAAAAATCCCGGCGCGAAGATCAT 
               
               
                   
               
               
                 CCACGATCCACGTCTCTCCTGGAACACCGTTGATGTGGTGACTGCCGCAGGTGGCACGCCGGTAATGTCGAAAAC 
               
               
                   
               
               
                 CGGACACGCCTTTATTAAAGAACGTATGCGCAAGGAAGACGCCATCTATGGTGGCGAAATGAGCGCCCACCATTA 
               
               
                   
               
               
                 CTTCCGTGATTTCGCTTACTGCGACAGCGGCATGATCCCGTGGCTGCTGGTCGCCGAACTGGTGTGCCTGAAAGA 
               
               
                   
               
               
                 TAAAACGCTGGGCGAACTGGTACGCGACCGGATGGCGGCGTTTCCGGCAAGCGGTGAGATCAACAGCAAACTGGC 
               
               
                   
               
               
                 GCAACCCGTTGAGGCGATTAACCGCGTGGAACAGCATTTTAGCCGTGAGGCGCTGGCGGTGGATCGCACCGATGG 
               
               
                   
               
               
                 CATCAGCATGACCTTTGCCGACTGGCGCTTTAACCTGCGCACCTCCAATACCGAACCGGTGGTGCGCCTGAATGT 
               
               
                   
               
               
                 GGAATCGCGCGGTGATGTGCCGCTGATGGAAGCGCGAACGCGAACTCTGCTGACGTTGCTGAACGAGTAATGTCG 
               
               
                   
               
               
                 GATCTTCCCTTACCCCACTGCGGGTAAGGGGCTAATAACAGGAACAACGATGATTCCGGGGATCCGTCGACCTGC 
               
               
                   
               
               
                 AGTTCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGAAGCAGCTCCAGCCTACAGTTAACAAAGCGGCATA 
               
               
                   
               
               
                 TTGATATGAGCTTACGTGAAAAAACCATCAGCGGCGCGAAGTGGTCGGCGATTGCCACGGTGATCATCATCGGCC 
               
               
                   
               
               
                 TCGGGCTGGTGCAGATGACCGTGCTGGCGCGGATTATCGACAACCACCAGTTCGGCCTGCTTACCGTGTCGCTGG 
               
               
                   
               
               
                 TGATTATCGCGCTGGCAGATACGCTTTCTGACTTCGGTATCGCTAACTCGATTATTCAGCGAAAAGAAATCAGTC 
               
               
                   
               
               
                 ACCTTGAACTCACCACGTTGTACTGGCTGAACGTCGGGCTGGGGATCGTGGTGTGCGTGGCGGTGTTTTTGTTGA 
               
               
                   
               
               
                 GTGATCTCATCGGCGACGTGCTGAATAACCCGGACCTGGCACCGTTGATTAAAACATTATCGCTGGCGTTTGTGG 
               
               
                   
               
               
                 TAATCCCCCACGGGCAACAGTTCCGCGCGTTGATGCAAAAAGAGCTGGAGTTCAACAAAATCGGCATGATCGAAA 
               
               
                   
               
               
                 CCAGCGCGGTGCTGGCGGGCTTCACTTGTACGGTGGTTAGCGCCCATTTCTGGCCGCTGGCGATGACCGCGATCC 
               
               
                   
               
               
                 TCGGTTATCTGGTCAATAGTGCGGTGAGAACGCTGCTGTTTGGCTACTTTGGCCGCAAAATTTATCGCCCCGGTC 
               
               
                   
               
               
                 TGCATTTCTCGCTGGCGTCGGTGGCACCGAACTTACGCTTTGGTGCCTGGCTGACGGCGGACAGCATCATCAACT 
               
               
                   
               
               
                 ATCTCAATACCAACCTTTCAACGCTCGTGCTGGCGCGTATTCTCGGCGCGGGCGTGGCAGGGGGATACAACCTGG 
               
               
                   
               
               
                 CGTACAACGTGGCCGTTGTGCCACCGATGAAGCTGAACCCAATCATCACCCGCGTGTTGTTTCCGGCATTCGCCA 
               
               
                   
               
               
                 AAATTCAGGACGATACCGAAAAGCTGCGTGTTAACTTCTACAAGCTGCTGTCGGTAGTGGGGATTATCAACTTTC 
               
               
                   
               
               
                 CGGCGCTGCTCGGGCTAATGGTGGTGTCGAATAACTTTGTACCGCTGGTCTTTGGTGAGAAGTGGAACAGCATTA 
               
               
                   
               
               
                 TTCCGGTGCTGCAATTGCTGTGTGTGGTGGGTCTGCTGCGCTCCG 
               
            
           
         
       
     
     Third, the magnitude of the cytoplasmic GDP-fucose pool was enhanced by the introduction of a null mutation into the lon gene. Lon is an ATP-dependant intracellular protease that is responsible for degrading RcsA, which is a positive transcriptional regulator of colanic acid biosynthesis in  E. coli  (Gottesman, S. &amp; Stout, V. Mol Microbiol 5, 1599-1606 (1991)). In a lon null background, RcsA is stabilized, RcsA levels increase, the genes responsible for GDP-fucose synthesis in  E. coli  are up-regulated, and intracellular GDP-fucose concentrations are enhanced. The lon gene was almost entirely deleted and replaced by an inserted functional, wild-type, but promoter-less  E. coli  lacZ +  gene (Δlon::(kan, lacZ + ). λ Red recombineering was used to perform the construction. A schematic of the kan, lacZ +  insertion into the lon locus is shown in  FIG.  14   . 
     Genomic DNA sequence surrounding the lacZ+ insertion into the lon region in the  E. coli  strain is set forth below (SEQ ID NO: 290): 
     
       
         
           
               
               
            
               
                 GTGGATGGAAGAGGTGGAAAAAGTGGTTATGGAGGAGTGGGTAATTGATGGTGAAAGGAAAGGGTTGGTGATTTA 
                   
               
               
                   
               
               
                 TGGGAAGGGGGAAGGGGAAGAGGGATGTGGTGAATAATTAAGGATTGGGATAGAATTAGTTAAGGAAAAAGGGGG 
               
               
                   
               
               
                 GATTTTATGTGGGGTTTAATTTTTGGTGTATTGTGGGGGTTGAATGTGGGGGAAAGATGGGGATATAGTGAGGTA 
               
               
                   
               
               
                 GATGTTAATAGATGGGGTGAAGGAGAGTGGTGTGATGTGATTAGGTGGGGGAAATTAAAGTAAGAGAGAGGTGTA 
               
               
                   
               
               
                 TGATTGGGGGGATGGGTGGAGGTGGAGTTGGAAGTTGGTATTGTGTAGAAAGTATAGGAAGTTGAGAGGGGTTTT 
               
               
                   
               
               
                 GAAGGTGAGGGTGGGGGAAGGAGTGAGGGGGGAAGGGGTGGTAAAGGAAGGGGAAGAGGTAGAAAGGGAGTGGGG 
               
               
                   
               
               
                 AGAAAGGGTGGTGAGGGGGGATGAATGTGAGGTAGTGGGGTATGTGGAGAAGGGAAAAGGGAAGGGGAAAGAGAA 
               
               
                   
               
               
                 AGGAGGTAGGTTGGAGTGGGGTTAGATGGGGATAGGTAGAGTGGGGGGTTTTATGGAGAGGAAGGGAAGGGGAAT 
               
               
                   
               
               
                 TGGGAGGTGGGGGGGGGTGTGGTAAGGTTGGGAAGGGGTGGAAAGTAAAGTGGATGGGTTTGTTGGGGGGAAGGA 
               
               
                   
               
               
                 TGTGATGGGGGAGGGGATGAAGATGTGATGAAGAGAGAGGATGAGGATGGTTTGGGATGATTGAAGAAGATGGAT 
               
               
                   
               
               
                 TGGAGGGAGGTTGTGGGGGGGGTTGGGTGGAGAGGGTATTGGGGTATGAGTGGGGAGAAGAGAGAATGGGGTGGT 
               
               
                   
               
               
                 GTGATGGGGGGGTGTTGGGGGTGTGAGGGGAGGGGGGGGGGGTTGTTTTTGTGAAGAGGGAGGTGTGGGGTGGGG 
               
               
                   
               
               
                 TGAATGAAGTGGAGGAGGAGGGAGGGGGGGTATGGTGGGTGGGGAGGAGGGGGGTTGGTTGGGGAGGTGTGGTGG 
               
               
                   
               
               
                 AGGTTGTGAGTGAAGGGGGAAGGGAGTGGGTGGTATTGGGGGAAGTGGGGGGGGAGGATGTGGTGTGATGTGAGG 
               
               
                   
               
               
                 TTGGTGGTGGGGAGAAAGTATGGATGATGGGTGATGGAATGGGGGGGGTGGATAGGGTTGATGGGGGTAGGTGGG 
               
               
                   
               
               
                 GATTGGAGGAGGAAGGGAAAGATGGGATGGAGGGAGGAGGTAGTGGGATGGAAGGGGGTGTTGTGGATGAGGATG 
               
               
                   
               
               
                 ATGTGGAGGAAGAGGATGAGGGGGTGGGGGGAGGGGAAGTGTTGGGGAGGGTGAAGGGGGGATGGGGGAGGGGGA 
               
               
                   
               
               
                 GGATGTGGTGGTGAGGGATGGGGATGGGTGGTTGGGGAATATGATGGTGGAAAATGGGGGGTTTTGTGGATTGAT 
               
               
                   
               
               
                 GGAGTGTGGGGGGGTGGGTGTGGGGGAGGGGTATGAGGAGATAGGGTTGGGTAGGGGTGATATTGGTGAAGAGGT 
               
               
                   
               
               
                 TGGGGGGGAATGGGGTGAGGGGTTGGTGGTGGTTTAGGGTATGGGGGGTGGGGATTGGGAGGGGATGGGGTTGTA 
               
               
                   
               
               
                 TGGGGTTGTTGAGGAGTTGTTGTAATAAGGGGATGTTGAAGTTGGTATTGGGAAGTTGGTATTGTGTAGAAAGTA 
               
               
                   
               
               
                 TAGGAAGTTGGAAGGAGGTGGAGGGTAGATAAAGGGGGGGGTTATTTTTGAGAGGAGAGGAAGTGGTAATGGTAG 
               
               
                   
               
               
                 GGAGGGGGGGTGAGGTGGAATTGGGGGGATAGTGAGGGGGTGGAGGAGTGGTGGGGAGGAATGGGGATATGGAAA 
               
               
                   
               
               
                 GGGTGGATATTGAGGGATGTGGGTTGTTGGGGGTGGAGGAGATGGGGATGGGTGGTTTGGATGAGTTGGTGTTGA 
               
               
                   
               
               
                 GTGTAGGGGGTGATGTTGAAGTGGAAGTGGGGGGGGGAGTGGTGTGGGGGATAATTGAATTGGGGGGTGGGGGAG 
               
               
                   
               
               
                 GGGAGAGGGTTTTGGGTGGGGAAGAGGTAGGGGGTATAGATGTTGAGAATGGGAGATGGGAGGGGTGAAAAGAGG 
               
               
                   
               
               
                 GGGGAGTAAGGGGGTGGGGATAGTTTTGTTGGGGGGGTAATGGGAGGGAGTTTAGGGGGTGTGGTAGGTGGGGGA 
               
               
                   
               
               
                 GGTGGGAGTTGAGGGGAATGGGGGGGGGATGGGGTGTATGGGTGGGGAGTTGAAGATGAAGGGTAATGGGGATTT 
               
               
                   
               
               
                 GAGGAGTAGGATGAATGGGGTAGGTTTTGGGGGTGATAAATAAGGTTTTGGGGTGATGGTGGGAGGGGTGAGGGG 
               
               
                   
               
               
                 TGGTAATGAGGAGGGGATGAGGAAGTGTATGTGGGGTGGAGTGGAAGAAGGGTGGTTGGGGGTGGTAATGGGGGG 
               
               
                   
               
               
                 GGGGGTTGGAGGGTTGGAGGGAGGGGTTAGGGTGAATGGGGGTGGGTTGAGTTAGGGGAATGTGGTTATGGAGGG 
               
               
                   
               
               
                 GTGGAGGGGTGAAGTGATGGGGGAGGGGGGTGAGGAGTTGTTTTTTATGGGGAATGGAGATGTGTGAAAGAAAGG 
               
               
                   
               
               
                 GTGAGTGGGGGTTAAATTGGGAAGGGTTATTAGGGAGGTGGATGGAAAAATGGATTTGGGTGGTGGTGAGATGGG 
               
               
                   
               
               
                 GGATGGGGTGGGAGGGGGGGGGGAGGGTGAGAGTGAGGTTTTGGGGGAGAGGGGAGTGGTGGGAGGGGGTGATGT 
               
               
                   
               
               
                 GGGGGGGTTGTGAGGATGGGGTGGGGTTGGGTTGGAGTAGGGGTAGTGTGAGGGAGAGTTGGGGGGGGGTGTGGG 
               
               
                   
               
               
                 GGTGGGGTAGTTGAGGGAGTTGAATGAAGTGTTTAGGTTGTGGAGGGAGATGGAGAGGGAGTTGAGGGGTTGGGA 
               
               
                   
               
               
                 GGGGGTTAGGATGGAGGGGGAGGATGGAGTGGAGGAGGTGGTTATGGGTATGAGGGAAGAGGTATTGGGTGGTGA 
               
               
                   
               
               
                 GTTGGATGGTTTGGGGGGATAAAGGGAAGTGGAAAAAGTGGTGGTGGTGTTTTGGTTGGGTGAGGGGTGGATGGG 
               
               
                   
               
               
                 GGGTGGGGTGGGGAAAGAGGAGAGGGTTGATAGAGAAGTGGGGATGGTTGGGGGTATGGGGAAAATGAGGGGGGT 
               
               
                   
               
               
                 AAGGGGAGGAGGGGTTGGGGTTTTGATGATATTTAATGAGGGAGTGATGGAGGGAGTGGGAGAGGAAGGGGGGGT 
               
               
                   
               
               
                 GTAAAGGGGGATAGTGAGGAAAGGGGTGGGAGTATTTAGGGAAAGGGGGAAGAGTGTTAGGGATGGGGTGGGGGT 
               
               
                   
               
               
                 ATTGGGAAAGGATGAGGGGGGGGGTGTGTGGAGGTAGGGAAAGGGATTTTTTGATGGAGGATTTGGGGAGAGGGG 
               
               
                   
               
               
                 GGAAGGGGTGGTGTTGATGGAGGGGGGGGTAGATGGGGGAAATAATATGGGTGGGGGTGGTGTGGGGTGGGGGGG 
               
               
                   
               
               
                 GTTGATAGTGGAGGGGGGGGGAAGGATGGAGAGATTTGATGGAGGGATAGAGGGGGTGGTGATTAGGGGGGTGGG 
               
               
                   
               
               
                 GTGATTGATTGGGGAGGGAGGAGATGATGAGAGTGGGGTGATTAGGATGGGGGTGGAGGATTGGGGTTAGGGGTT 
               
               
                   
               
               
                 GGGTGATGGGGGGTAGGGAGGGGGGATGATGGGTGAGAGGATTGATTGGGAGGATGGGGTGGGTTTGAATATTGG 
               
               
                   
               
               
                 GTTGATGGAGGAGATAGAGGGGGTAGGGGTGGGAGAGGGTGTAGGAGAGGGGATGGTTGGGATAATGGGAAGAGG 
               
               
                   
               
               
                 GGAGGGGGTTAAAGTTGTTGTGGTTGATGAGGAGGATATGGTGGAGGATGGTGTGGTGATGGATGAGGTGAGGAT 
               
               
                   
               
               
                 GGAGAGGATGATGGTGGTGAGGGTTAAGGGGTGGAATGAGGAAGGGGTTGGGGTTGAGGAGGAGGAGAGGATTTT 
               
               
                   
               
               
                 GAATGGGGAGGTGGGGGAAAGGGAGATGGGAGGGTTGTGGTTGAATGAGGGTGGGGTGGGGGGTGTGGAGTTGAA 
               
               
                   
               
               
                 GGAGGGGAGGATAGAGATTGGGGATTTGGGGGGTGGAGAGTTTGGGGTTTTGGAGGTTGAGAGGTAGTGTGAGGG 
               
               
                   
               
               
                 GATGGGGATAAGGAGGAGGGTGATGGATAATTTGAGGGGGGAAAGGGGGGGTGGGGGTGGGGAGGTGGGTTTGAG 
               
               
                   
               
               
                 GGTGGGATAAAGAAAGTGTTAGGGGTAGGTAGTGAGGGAAGTGGGGGGAGATGTGAAGTTGAGGGTGGAGTAGAG 
               
               
                   
               
               
                 GGGGGGTGAAATGATGATTAAAGGGAGTGGGAAGATGGAAATGGGTGATTTGTGTAGTGGGTTTATGGAGGAAGG 
               
               
                   
               
               
                 AGAGGTGAGGGAAAATGGGGGTGATGGGGGAGATATGGTGATGTTGGAGATAAGTGGGGTGAGTGGAGGGGAGGA 
               
               
                   
               
               
                 GGATGAGGGGGAGGGGGTTTTGTGGGGGGGGTAAAAATGGGGTGAGGTGAAATTGAGAGGGGAAAGGAGTGTGGT 
               
               
                   
               
               
                 GGGGGTAAGGGAGGGAGGGGGGGTTGGAGGAGAGATGAAAGGGGGAGTTAAGGGGATGAAAAATAATTGGGGTGT 
               
               
                   
               
               
                 GGGGTTGGTGTAGGGAGGTTTGATGAAGATTAAATGTGAGGGAGTAAGAAGGGGTGGGATTGTGGGTGGGAAGAA 
               
               
                   
               
               
                 AGGGGGGATTGAGGGTAATGGGATAGGTGAGGTTGGTGTAGATGGGGGGATGGTAAGGGTGGATGTGGGAGTTTG 
               
               
                   
               
               
                 AGGGGAGGAGGAGAGTATGGGGGTGAGGAAGATGGGAGGGAGGGAGGTTTGGGGGAGGGGTTGTGGTGGGGGAAA 
               
               
                   
               
               
                 GGAGGGAAAGGGGGATTGGGGATTGAGGGTGGGGAAGTGTTGGGAAGGGGGATGGGTGGGGGGGTGTTGGGTATT 
               
               
                   
               
               
                 AGGGGAGGTGGGGAAAGGGGGATGTGGTGGAAGGGGATTAAGTTGGGTAAGGGGAGGGTTTTGGGAGTGAGGAGG 
               
               
                   
               
               
                 TTGTAAAAGGAGGGGGAGTGAATGGGTAATGATGGTGATAGTAGGTTTGGTGAGGTTGTGAGTGGAAAATAGTGA 
               
               
                   
               
               
                 GGTGGGGGAAAATGGAGTAATAAAAAGAGGGGTGGGAGGGTAATTGGGGGTTGGGAGGGTTTTTTTGTGTGGGTA 
               
               
                   
               
               
                 AGTTAGATGGGGGATGGGGGTTGGGGTTATTAAGGGGTGTTGTAAGGGGATGGGTGGGGTGATATAAGTGGTGGG 
               
               
                   
               
               
                 GGTTGGTAGGTTGAAGGATTGAAGTGGGATATAAATTATAAAGAGGAAGAGAAGAGTGAATAAATGTGAATTGAT 
               
               
                   
               
               
                 GGAGAAGATTGGTGGAGGGGGTGATATGTGTAAAGGTGGGGGTGGGGGTGGGTTAGATGGTATTATTGGTTGGGT 
               
               
                   
               
               
                 AAGTGAATGTGTGAAAGAAGG 
               
            
           
         
       
     
     Fourth, a thyA (thymidylate synthase) mutation was introduced into the strain by P1 transduction. In the absence of exogenous thymidine, thyA strains are unable to make DNA and die. The defect can be complemented in trans by supplying a wild-type thyA gene on a multicopy plasmid (Belfort, M., Maley, G. F., and Maley, F. (1983). Proc Natl Acad Sci USA 80, 1858-861). This complementation was used here as a means of plasmid maintenance. 
     An additional modification that is useful for increasing the cytoplasmic pool of free lactose (and hence the final yield of 2′-FL) is the incorporation of a lacA mutation. LacA is a lactose acetyltransferase that is only active when high levels of lactose accumulate in the  E. coli  cytoplasm. High intracellular osmolarity (e.g., caused by a high intracellular lactose pool) can inhibit bacterial growth, and  E. coli  has evolved a mechanism for protecting itself from high intra cellular osmlarity caused by lactose by “tagging” excess intracellular lactose with an acetyl group using LacA, and then actively expelling the acetyl-lactose from the cell (Danchin, A. Bioessays 31, 769-773 (2009)). Production of acetyl-lactose in  E. coli  engineered to produce 2′-FL or other human milk oligosaccharides is therefore undesirable: it reduces overall yield. Moreover, acetyl-lactose is a side product that complicates oligosaccharide purification schemes. The incorporation of a lacA mutation resolves these problems. Sub-optimal production of fucosylated oligosaccharides occurs in strains lacking either or both of the mutations in the colanic acid pathway and the lon protease. Diversion of lactose into a side product (acetyl-lactose) occurs in strains that do not contain the lacA mutation. A schematic of the lacA deletion and corresponding genomic sequence is provided above (SEQ ID NO: 288). 
     The strain used to test the different α(1,2) FT candidates incorporates all the above genetic modifications and has the following genotype: ΔampC::P trp   B cI, A(lacI-lacZ)::FRT, P lacIq lacY + , ΔwcaJ::FRT, thyA::Tn10, Δlon:(npt3, lacZ + ), ΔlacA 
     The  E. coli  strains harboring the different α(1,2) FT candidate expression plasmids were analyzed. Strains were grown in selective media (lacking thymidine) to early exponential phase. Lactose was then added to a final concentration of 0.5%, and tryptophan (200 μM) was added to induce expression of each candidate α(1,2) FT from the P L  promoter. At the end of the induction period (˜24 h) equivalent OD 600 units of each strain and the culture supernatant was harvested. Lysates were prepared and analyzed for the presence of 2′-FL by thin layer chromatography (TLC). 
     A map of plasmid pG217 is shown in  FIG.  11   , which carries the  B. vulgatus  FutN. The sequence of plasmid pG217 is set forth below (SEQ ID NO: 291): 
     
       
         
           
               
               
            
               
                 TCTAGAATTCTAAAAATTGATTGAATGTATGCAAATAAATGCATACACCATAGGTGTGGTTTAATTTGATGCCCT 
                   
               
               
                   
               
               
                 TTTTCAGGGCTGGAATGTGTAAGAGCGGGGTTATTTATGCTGTTGTTTTTTTGTTACTCGGGAAGGGCTTTACCT 
               
               
                   
               
               
                 CTTCCGCATAAACGCTTCCATCAGCGTTTATAGTTAAAAAAATCTTTCGGAACTGGTTTTGCGCTTACCCCAACC 
               
               
                   
               
               
                 AACAGGGGATTTGCTGCTTTCCATTGAGCCTGTTTCTCTGCGCGACGTTCGCGGCGGCGTGTTTGTGCATCCATC 
               
               
                   
               
               
                 TGGATTCTCCTGTCAGTTAGCTTTGGTGGTGTGTGGCAGTTGTAGTCCTGAACGAAAACCCCCCGCGATTGGCAC 
               
               
                   
               
               
                 ATTGGCAGCTAATCCGGAATCGCACTTACGGCCAATGCTTCGTTTCGTATCACACACCCCAAAGCCTTCTGCTTT 
               
               
                   
               
               
                 GAATGCTGCCCTTCTTCAGGGCTTAATTTTTAAGAGCGTCACCTTCATGGTGGTCAGTGCGTCCTGCTGATGTGC 
               
               
                   
               
               
                 TCAGTATCACCGCCAGTGGTATTTATGTCAACACCGCCAGAGATAATTTATCACCGCAGATGGTTATCTGTATGT 
               
               
                   
               
               
                 TTTTTATATGAATTTATTTTTTGCAGGGGGGCATTGTTTGGTAGGTGAGAGATCAATTCTGCATTAATGAATCGG 
               
               
                   
               
               
                 CCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGT 
               
               
                   
               
               
                 CGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGC 
               
               
                   
               
               
                 AGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCA 
               
               
                   
               
               
                 TAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATA 
               
               
                   
               
               
                 AAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCT 
               
               
                   
               
               
                 GTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGT 
               
               
                   
               
               
                 CGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCG 
               
               
                   
               
               
                 TCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAG 
               
               
                   
               
               
                 GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTAT 
               
               
                   
               
               
                 CTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGG 
               
               
                   
               
               
                 TAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTT 
               
               
                   
               
               
                 TTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGAT 
               
               
                   
               
               
                 CTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGA 
               
               
                   
               
               
                 CAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACT 
               
               
                   
               
               
                 CCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCC 
               
               
                   
               
               
                 ACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAAC 
               
               
                   
               
               
                 TTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCG 
               
               
                   
               
               
                 CAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTC 
               
               
                   
               
               
                 CCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGT 
               
               
                   
               
               
                 TGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCC 
               
               
                   
               
               
                 ATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAG 
               
               
                   
               
               
                 TTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAA 
               
               
                   
               
               
                 ACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACC 
               
               
                   
               
               
                 CAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAA 
               
               
                   
               
               
                 AAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCA 
               
               
                   
               
               
                 GGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATT 
               
               
                   
               
               
                 TCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCAC 
               
               
                   
               
               
                 GAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCAC 
               
               
                   
               
               
                 AGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGG 
               
               
                   
               
               
                 CTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGA 
               
               
                   
               
               
                 TGCGTAAGGAGAAAATACCGCATCAGGCGCCTCCTCAACCTGTATATTCGTAAACCACGCCCAATGGGAGCTGTC 
               
               
                   
               
               
                 TCAGGTTTGTTCCTGATTGGTTACGGCGCGTTTCGCATCATTGTTGAGTTTTTCCGCCAGCCCGACGCGCAGTTT 
               
               
                   
               
               
                 ACCGGTGCCTGGGTGCAGTACATCAGCATGGGGCAAATTCTTTCCATCCCGATGATTGTCGCGGGTGTGATCATG 
               
               
                   
               
               
                 ATGGTCTGGGCATATCGTCGCAGCCCACAGCAACACGTTTCCTGAGGAACCATGAAACAGTATTTAGAACTGATG 
               
               
                   
               
               
                 CAAAAAGTGCTCGACGAAGGCACACAGAAAAACGACCGTACCGGAACCGGAACGCTTTCCATTTTTGGTCATCAG 
               
               
                   
               
               
                 ATGCGTTTTAACCTGCAAGATGGATTCCCGCTGGTGACAACTAAACGTTGCCACCTGCGTTCCATCATCCATGAA 
               
               
                   
               
               
                 CTGCTGTGGTTTCTGCAGGGCGACACTAACATTGCTTATCTACACGAAAACAATGTCACCATCTGGGACGAATGG 
               
               
                   
               
               
                 GCCGATGAAAACGGCGACCTCGGGCCAGTGTATGGTAAACAGTGGCGCGCCTGGCCAACGCCAGATGGTCGTCAT 
               
               
                   
               
               
                 ATTGACCAGATCACTACGGTACTGAACCAGCTGAAAAACGACCCGGATTCGCGCCGCATTATTGTTTCAGCGTGG 
               
               
                   
               
               
                 AACGTAGGCGAACTGGATAAAATGGCGCTGGCACCGTGCCATGCATTCTTCCAGTTCTATGTGGCAGACGGCAAA 
               
               
                   
               
               
                 CTCTCTTGCCAGCTTTATCAGCGCTCCTGTGACGTCTTCCTCGGCCTGCCGTTCAACATTGCCAGCTACGCGTTA 
               
               
                   
               
               
                 TTGGTGCATATGATGGCGCAGCAGTGCGATCTGGAAGTGGGTGATTTTGTCTGGACCGGTGGCGACACGCATCTG 
               
               
                   
               
               
                 TACAGCAACCATATGGATCAAACTCATCTGCAATTAAGCCGCGAACCGCGTCCGCTGCCGAAGTTGATTATCAAA 
               
               
                   
               
               
                 CGTAAACCCGAATCCATCTTCGACTACCGTTTCGAAGACTTTGAGATTGAAGGCTACGATCCGCATCCGGGCATT 
               
               
                   
               
               
                 AAAGCGCCGGTGGCTATCTAATTACGAAACATCCTGCCAGAGCCGACGCCAGTGTGCGTCGGTTTTTTTACCCTC 
               
               
                   
               
               
                 CGTTAAATTCTTCGAGACGCCTTCCCGAAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATC 
               
               
                   
               
               
                 GGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCC 
               
               
                   
               
               
                 AGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTTCTTTAATGAAGCAGGGCATCAGGAC 
               
               
                   
               
               
                 GGTATCTTTGTGGAGAAAGCAGAGTAATCTTATTCAGCCTGACTGGTGGGAAACCACCAGTCAGAATGTGTTAGC 
               
               
                   
               
               
                 GCATGTTGACAAAAATACCATTAGTCACATTATCCGTCAGTCGGACGACATGGTAGATAACCTGTTTATTATGCG 
               
               
                   
               
               
                 TTTTGATCTTACGTTTAATATTACCTTTATGCGATGAAACGGTCTTGGCTTTGATATTCATTTGGTCAGAGATTT 
               
               
                   
               
               
                 GAATGGTTCCCTGACCTGCCATCCACATTCGCAACATACTCGATTCGGTTCGGCTCAATGATAACGTCGGCATAT 
               
               
                   
               
               
                 TTAAAAACGAGGTTATCGTTGTCTCTTTTTTCAGAATATCGCCAAGGATATCGTCGAGAGATTCCGGTTTAATCG 
               
               
                   
               
               
                 ATTTAGAACTGATCAATAAATTTTTTCTGACCAATAGATATTCATCAAAATGAACATTGGCAATTGCCATAAAAA 
               
               
                   
               
               
                 CGATAAATAACGTATTGGGATGTTGATTAATGATGAGCTTGATACGCTGACTGTTAGAAGCATCGTGGATGAAAC 
               
               
                   
               
               
                 AGTCCTCATTAATAAACACCACTGAAGGGCGCTGTGAATCACAAGCTATGGCAAGGTCATCAACGGTTTCAATGT 
               
               
                   
               
               
                 CGTTGATTTCTCTTTTTTTAACCCCTCTACTCAACAGATACCCGGTTAAACCTAGTCGGGTGTAACTACATAAAT 
               
               
                   
               
               
                 CCATAATAATCGTTGACATGGCATACCCTCACTCAATGCGTAACGATAATTCCCCTTACCTGAATATTTCATCAT 
               
               
                   
               
               
                 GACTAAACGGAACAACATGGGTCACCTAATGCGCCACTCTCGCGATTTTTCAGGCGGACTTACTATCCCGTAAAG 
               
               
                   
               
               
                 TGTTGTATAATTTGCCTGGAATTGTCTTAAAGTAAAGTAAATGTTGCGATATGTGAGTGAGCTTAAAACAAATAT 
               
               
                   
               
               
                 TTCGCTGCAGGAGTATCCTGGAAGATGTTCGTAGAAGCTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGT 
               
               
                   
               
               
                 GCCTTTTTTATTTGTACTACCCTGTACGATTACTGCAGCTCGAGTTAGGATACCGGCACTTTGATCCAACCAGTC 
               
               
                   
               
               
                 GGGTAGATATCCGGTGCTTCGGAGTGCTGGAACCAACGGCTCGGCACAATAACAGTCTTATCCATATTAGGGTTC 
               
               
                   
               
               
                 AGCCAGGCACCCCACCAAGAAAACGTGCTGTTACAAATGATGTGATGTTTGCAATGAGACATCAGCATCATATCC 
               
               
                   
               
               
                 TGCCAGGAGTCTTCATCAGTGTTCCAGTCAATATAAACCGCATTCTGCAGTGGCAGATTTTCTTTAACCCACGCG 
               
               
                   
               
               
                 ATATCGTCGGAGAAGATATAGTAAGATGGGCTAGCAACACGACGGGACATTTCCGCGATAGCATTCTGGTAATAC 
               
               
                   
               
               
                 GGCAGCTGGCACACGGAACCGGTAGTAGCCCAGTGTTTCGGCTGCAGATAGTCACCACGACGAATGTGCAGGGAA 
               
               
                   
               
               
                 ACCGCGTTTTCATCTTTGTCCAGGATTTCCAGCATGTTCAGGCTGCGGGAATTTGCTTTGTTCTTATCAAAGGTG 
               
               
                   
               
               
                 AAGGATTCACGCACTTCGTCTTTGATATCAGCGAAGAAACGCTCGCTCTGATAGAAACCTTTAAAGTACAGCAGC 
               
               
                   
               
               
                 GGCCAGAAATACTTCTTCTCGAACGCACGCAGAGAGTTCGGCGCCTGCTTGCGTTCGTAGATTTTTTTAAAAAAC 
               
               
                   
               
               
                 AGGAATTCGATAACTTTTTTCAGCGGTTGGTTGATGCAGAATTCGGTGTGCGGCAGGTTGAACACGCGGTGCATT 
               
               
                   
               
               
                 TCGTAACCGTAATGGACTTTGTAATGCATCATGTCGCTCAGGTCGATACGGACCTTCGGGTAATACTTTTTCATA 
               
               
                   
               
               
                 CGCAGATAGAAAGCATAGATAAACATCTGGTTGCCCAGACCGCCAGTCACTTTGATCAGACGCATTATATCTCCT 
               
               
                   
               
               
                 TCTTG 
               
            
           
         
       
     
     Fucosylated oligosaccharides produced by metabolically engineered  E. coli  cells are purified from culture broth post-fermentation. An exemplary procedure comprises five steps. (1) Clarification: Fermentation broth is harvested and cells removed by sedimentation in a preparative centrifuge at 6000×g for 30 min. Each bioreactor run yields about 5-7 L of partially clarified supernatant. (2) Product capture on coarse carbon: A column packed with coarse carbon (Calgon 12×40 TR) of ˜4000 ml volume (dimension 5 cm diameter×60 cm length) is equilibrated with 1 column volume (CV) of water and loaded with clarified culture supernatant at a flow rate of 40 ml/min. This column has a total capacity of about 120 g of sugar. Following loading and sugar capture, the column is washed with 1.5 CV of water, then eluted with 2.5 CV of 50% ethanol or 25% isopropanol (lower concentrations of ethanol at this step (25-30%) may be sufficient for product elution.) This solvent elution step releases about 95% of the total bound sugars on the column and a small portion of the color bodies. In this first step capture of the maximal amount of sugar is the primary objective. Resolution of contaminants is not an objective. (3) Evaporation: A volume of 2.5 L of ethanol or isopropanol eluate from the capture column is rotary-evaporated at 56 C.° and a sugar syrup in water is generated. Alternative methods that could be used for this step include lyophilization or spray-drying. (4) Flash chromatography on fine carbon and ion exchange media: A column (GE Healthcare HiScale50/40, 5×40 cm, max pressure 20 bar) connected to a Biotage Isolera One FLASH Chromatography System is packed with 750 ml of a Darco Activated Carbon G60 (100-mesh): Celite 535 (coarse) 1:1 mixture (both column packings were obtained from Sigma). The column is equilibrated with 5 CV of water and loaded with sugar from step 3 (10-50 g, depending on the ratio of 2′-FL to contaminating lactose), using either a celite loading cartridge or direct injection. The column is connected to an evaporative light scattering (ELSD) detector to detect peaks of eluting sugars during the chromatography. A four-step gradient of isopropanol, ethanol or methanol is run in order to separate 2′-FL from monosaccharides (if present), lactose and color bodies. Fractions corresponding to sugar peaks are collected automatically in 120-ml bottles, pooled and directed to step 5. In certain purification runs from longer-than-normal fermentations, passage of the 2′-FL-containing fraction through anion-exchange and cation exchange columns can remove excess protein/DNA/caramel body contaminants. Resins tested successfully for this purpose are Dowex 22. 
     The gene screening approach described herein was successfully utilized to identify new α(1,2) FTs for the efficient biosynthesis of 2′-FL in metabolically engineered  E. coli  host strains. The results of the screen are summarized in Table 1. 
     Production Host Strains 
       E. coli  K-12 is a well-studied bacterium which has been the subject of extensive research in microbial physiology and genetics and commercially exploited for a variety of industrial uses. The natural habitat of the parent species,  E. coli , is the large bowel of mammals.  E. coli  K-12 has a history of safe use, and its derivatives are used in a large number of industrial applications, including the production of chemicals and drugs for human administration and consumption.  E. coli  K-12 was originally isolated from a convalescent diphtheria patient in 1922. Because it lacks virulence characteristics, grows readily on common laboratory media, and has been used extensively for microbial physiology and genetics research, it has become the standard bacteriological strain used in microbiological research, teaching, and production of products for industry and medicine.  E. coli  K-12 is now considered an enfeebled organism as a result of being maintained in the laboratory environment for over 70 years. As a result, K-12 strains are unable to colonize the intestines of humans and other animals under normal conditions. Additional information on this well known strain is available at http://epa.gov/oppt/biotech/pubs/fra/fra004.htm. In addition to  E. coli  K12, other bacterial strains are used as production host strains, e.g., a variety of bacterial species may be used in the oligosaccharide biosynthesis methods, e.g.,  Erwinia herbicola  ( Pantoea agglomerans ),  Citrobacter freundii, Pantoea citrea, Pectobacterium carotovorum , or  Xanthomonas campestris . Bacteria of the genus  Bacillus  may also be used, including  Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, Bacillus thermophilus, Bacillus laterosporus, Bacillus megaterium, Bacillus mycoides, Bacillus pumilus, Bacillus lentus, Bacillus cereus , and  Bacillus circulans . Similarly, bacteria of the genera  Lactobacillus  and  Lactococcus  may be modified using the methods of this invention, including but not limited to  Lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus helveticus, Lactobacillus delbrueckii, Lactobacillus rhamnosus, Lactobacillus bulgaricus, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus casei, Lactobacillus reuteri, Lactobacillus jensenii , and  Lactococcus lactis. Streptococcus thermophiles  and  Proprionibacterium freudenreichii  are also suitable bacterial species for the invention described herein. Also included as part of this invention are strains, modified as described here, from the genera  Enterococcus  (e.g.,  Enterococcus faecium  and  Enterococcus thermophiles ),  Bifidobacterium  (e.g.,  Bifidobacterium longum, Bifidobacterium infantis , and  Bifidobacterium bifidum ),  Sporolactobacillus  spp.,  Micromomospora  spp.,  Micrococcus  spp.,  Rhodococcus  spp., and  Pseudomonas  (e.g.,  Pseudomonas fluorescens  and  Pseudomonas aeruginosa ). 
     Suitable host strains are amenable to genetic manipulation, e.g., they maintain expression constructs, accumulate precursors of the desired end product, e.g., they maintain pools of lactose and GDP-fucose, and accumulate endproduct, e.g., 2′-FL. Such strains grow well on defined minimal media that contains simple salts and generally a single carbon source. The strains engineered as described above to produce the desired fucosylated oligosaccharide(s) are grown in a minimal media. An exemplary minimal medium used in a bioreactor, minimal “FERM” medium, is detailed below. 
     Ferm (10 liters): Minimal medium comprising: 
     40 g (NH 4 ) 2 HPO 4    
     100 g KH 2 PO 4    
     10 g MgSO 4 .7H 2 O 
     40 g NaOH 
     1× Trace elements: 
     1.3 g NTA (nitrilotriacetic acid) 
     0.5 g FeSO 4 .7H 2 O 
     0.09 g MnCl 2 .4H 2 O 
     0.09 g ZnSO 4 .7H 2 O 
     0.01 g CoCl 2 .6H 2 O 
     0.01 g CuCl 2 .2H 2 O 
     0.02 g H 3 BO 3    
     0.01 g Na 2 MoO 4 .2H 2 O (pH 6.8) 
     Water to 10 liters 
     DF204 antifoam (0.1 ml/L) 
     150 g glycerol (initial batch growth), followed by fed batch mode with a 90% glycerol-1% MgSO 4 -1× trace elements feed, at various rates for various times. 
     A suitable production host strain is one that is not the same bacterial strain as the source bacterial strain from which the fucosyltransferase-encoding nucleic acid sequence was identified. 
     Bacteria comprising the characteristics described herein are cultured in the presence of lactose, and a fucosylated oligosaccharide is retrieved, either from the bacterium itself or from a culture supernatant of the bacterium. The fucosylated oligosaccharide is purified for use in therapeutic or nutritional products, or the bacteria are used directly in such products. 
     EXAMPLES 
     Example 1: Identification of Novel α(1,2) Fucosyltransferases 
     To identify additional novel α(1,2)fucosyltransferases, a multiple sequence alignment query was generated using the alignment algorithm of the CLCbio Main Workbench package, version 6.9 (CLCbio, 10 Rogers Street #101, Cambridge, Mass. 02142, USA) using four previously identified lactose-utilizing α(1,2)fucosyltransferase protein sequences:  H. pylori  futC (SEQ ID NO: 1),  H. mustelae  FutL (SEQ ID NO: 2),  Bacteroides  vulgatus futN (SEQ ID NO: 3), and  E. coli  0126 wbgL (SEQ ID NO: 4). This sequence alignment and percentages of sequence identity between the four previously identified lactose-utilizing α(1,2)fucosyltransferase protein sequences is shown in  FIG.  3   . An iterative PSI-BLAST was performed, using the FASTA-formatted multiple sequence alignment as the query, and the NCBI PSI-BLAST program run on a local copy of NCBI BLAST+ version 2.2.29. An initial position-specific scoring matrix file (.pssm) was generated by PSI-BLAST, which was then used to adjust the score of iterative homology search runs. The process is iterated to generate an even larger group of candidates, and the results of each run were used to further refine the matrix. 
     A portion of the initial position-specific scoring matrix file used is shown below: 
     
       
         
           
               
            
               
                   
               
               
                 Last position-specific scoring matrix computed 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                 A 
                 R 
                 N 
                 D 
                 C 
                 Q 
                 E 
                 G 
                 H 
                 I 
                 L 
                 K 
                 M 
                 F 
                 P 
                 S 
                 T 
                 W 
                 Y 
                 V 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                  1 
                 M 
                 −1 
                 −1 
                 −2 
                 −3 
                 −2 
                 0 
                 −2 
                 −3 
                 −2 
                 1 
                 2 
                 −1 
                 6 
                 0 
                 −3 
                 −2 
                 −1 
                 −2 
                 −1 
                 1 
               
               
                  2 
                 A 
                 2 
                 −2 
                 0 
                 4 
                 −2 
                 −1 
                 1 
                 −1 
                 −1 
                 −2 
                 −3 
                 −1 
                 −2 
                 −3 
                 −1 
                 1 
                 −1 
                 −3 
                 −3 
                 −1 
               
               
                  3 
                 F 
                 −2 
                 −3 
                 −3 
                 −4 
                 −3 
                 −3 
                 −3 
                 −3 
                 −1 
                 0 
                 0 
                 −3 
                 0 
                 7 
                 −4 
                 −3 
                 −2 
                 1 
                 1 
                 −1 
               
               
                  4 
                 K 
                 0 
                 3 
                 0 
                 −3 
                 −2 
                 1 
                 0 
                 −1 
                 −1 
                 −3 
                 −3 
                 3 
                 −2 
                 −3 
                 −1 
                 2 
                 0 
                 −3 
                 −2 
                 −2 
               
               
                  5 
                 V 
                 −1 
                 −3 
                 −3 
                 −4 
                 −1 
                 −3 
                 −3 
                 −4 
                 −3 
                 4 
                 2 
                 −3 
                 1 
                 0 
                 −3 
                 −2 
                 −1 
                 −3 
                 −1 
                 3 
               
               
                  6 
                 V 
                 −1 
                 −3 
                 −3 
                 −3 
                 −1 
                 −3 
                 −3 
                 −4 
                 −3 
                 4 
                 1 
                 −3 
                 1 
                 −1 
                 −3 
                 −2 
                 0 
                 −3 
                 −1 
                 3 
               
               
                  7 
                 Q 
                 −1 
                 4 
                 0 
                 −1 
                 −3 
                 4 
                 1 
                 −2 
                 0 
                 −3 
                 −2 
                 3 
                 −1 
                 −3 
                 −2 
                 0 
                 −1 
                 −3 
                 −2 
                 −3 
               
               
                  8 
                 I 
                 −1 
                 −3 
                 −3 
                 −4 
                 −1 
                 −2 
                 −3 
                 −4 
                 −3 
                 3 
                 2 
                 −3 
                 1 
                 0 
                 −3 
                 −2 
                 −1 
                 −3 
                 −1 
                 3 
               
               
                  9 
                 C 
                 −1 
                 −1 
                 0 
                 −1 
                 5 
                 3 
                 6 
                 −2 
                 4 
                 −2 
                 −2 
                 0 
                 −1 
                 −2 
                 −2 
                 0 
                 2 
                 −2 
                 −1 
                 −1 
               
               
                 10 
                 G 
                 0 
                 −3 
                 −1 
                 −1 
                 −3 
                 −2 
                 −2 
                 6 
                 −2 
                 −4 
                 −4 
                 −2 
                 −3 
                 −3 
                 −2 
                 0 
                 −2 
                 −3 
                 −3 
                 −3 
               
               
                 11 
                 G 
                 0 
                 −3 
                 −1 
                 −1 
                 −3 
                 −2 
                 −2 
                 6 
                 −2 
                 −4 
                 −4 
                 −2 
                 −3 
                 −3 
                 −2 
                 0 
                 −2 
                 −3 
                 −3 
                 −3 
               
               
                 12 
                 L 
                 −2 
                 −2 
                 −4 
                 −4 
                 −1 
                 −2 
                 −3 
                 −4 
                 −3 
                 2 
                 4 
                 −3 
                 2 
                 0 
                 −3 
                 −3 
                 −1 
                 −2 
                 −1 
                 1 
               
               
                 13 
                 G 
                 0 
                 −3 
                 −1 
                 −1 
                 −3 
                 −2 
                 −2 
                 6 
                 −2 
                 −4 
                 −4 
                 −2 
                 −3 
                 −3 
                 −2 
                 0 
                 −2 
                 −3 
                 −3 
                 −3 
               
               
                 14 
                 N 
                 −2 
                 −1 
                 6 
                 1 
                 −3 
                 0 
                 0 
                 −1 
                 1 
                 −4 
                 −4 
                 0 
                 −2 
                 −3 
                 −2 
                 1 
                 0 
                 −4 
                 −2 
                 −3 
               
               
                 15 
                 Q 
                 −1 
                 1 
                 0 
                 0 
                 −3 
                 6 
                 2 
                 −2 
                 0 
                 −3 
                 −2 
                 1 
                 −1 
                 −3 
                 −1 
                 0 
                 −1 
                 −2 
                 −2 
                 −2 
               
               
                 16 
                 N 
                 −1 
                 −2 
                 −3 
                 −4 
                 −2 
                 −1 
                 −2 
                 −3 
                 −2 
                 1 
                 3 
                 −2 
                 5 
                 0 
                 −3 
                 −2 
                 −1 
                 −2 
                 −1 
                 1 
               
               
                 17 
                 F 
                 −2 
                 −3 
                 −3 
                 −4 
                 −3 
                 −3 
                 −4 
                 −3 
                 −1 
                 0 
                 0 
                 −3 
                 0 
                 7 
                 −4 
                 −3 
                 −2 
                 1 
                 3 
                 −1 
               
               
                 18 
                 Q 
                 −1 
                 0 
                 −1 
                 −1 
                 −3 
                 5 
                 1 
                 −2 
                 0 
                 1 
                 −1 
                 1 
                 0 
                 −2 
                 −2 
                 −1 
                 −1 
                 −2 
                 −2 
                 0 
               
               
                 19 
                 Y 
                 −2 
                 −2 
                 −3 
                 −3 
                 −3 
                 −2 
                 −3 
                 −3 
                 1 
                 −1 
                 −1 
                 −2 
                 −1 
                 5 
                 −3 
                 −2 
                 −2 
                 2 
                 6 
                 −1 
               
               
                 20 
                 A 
                 4 
                 −1 
                 −1 
                 −1 
                 −1 
                 −1 
                 −1 
                 0 
                 −2 
                 −2 
                 −2 
                 −1 
                 −1 
                 −2 
                 −1 
                 2 
                 0 
                 −3 
                 −2 
                 −1 
               
               
                 21 
                 F 
                 −2 
                 −3 
                 −3 
                 −4 
                 −3 
                 −3 
                 −4 
                 −3 
                 −1 
                 0 
                 0 
                 −3 
                 0 
                 2 
                 −4 
                 −3 
                 −2 
                 1 
                 3 
                 −1 
               
               
                 22 
                 A 
                 3 
                 −2 
                 −1 
                 −2 
                 −1 
                 −1 
                 −1 
                 4 
                 −2 
                 −2 
                 −2 
                 −1 
                 −2 
                 −3 
                 −1 
                 1 
                 −1 
                 −3 
                 −2 
                 −1 
               
               
                 23 
                 K 
                 −1 
                 0 
                 −1 
                 −2 
                 −3 
                 0 
                 −1 
                 −3 
                 −1 
                 −2 
                 −2 
                 3 
                 −1 
                 2 
                 −2 
                 −1 
                 −1 
                 1 
                 5 
                 −2 
               
               
                 24 
                 S 
                 2 
                 −1 
                 −1 
                 −2 
                 −1 
                 −1 
                 −1 
                 −1 
                 −2 
                 −1 
                 1 
                 −1 
                 0 
                 −1 
                 −1 
                 3 
                 0 
                 −3 
                 −2 
                 0 
               
               
                 25 
                 L 
                 −2 
                 3 
                 −2 
                 −3 
                 −2 
                 −1 
                 −2 
                 −3 
                 −2 
                 1 
                 3 
                 0 
                 1 
                 0 
                 −3 
                 −2 
                 −1 
                 −2 
                 −1 
                 0 
               
               
                 26 
                 Q 
                 0 
                 0 
                 0 
                 −1 
                 −2 
                 4 
                 1 
                 −2 
                 −1 
                 −1 
                 0 
                 0 
                 3 
                 −2 
                 −2 
                 2 
                 0 
                 −2 
                 −2 
                 −1 
               
               
                 27 
                 K 
                 −1 
                 2 
                 0 
                 −1 
                 −3 
                 1 
                 0 
                 −2 
                 −1 
                 −2 
                 −2 
                 4 
                 −1 
                 −3 
                 −1 
                 0 
                 2 
                 −3 
                 −2 
                 −2 
               
               
                 28 
                 H 
                 −1 
                 0 
                 0 
                 −2 
                 −3 
                 0 
                 0 
                 −2 
                 6 
                 1 
                 −1 
                 2 
                 −1 
                 −1 
                 −2 
                 −1 
                 −1 
                 −3 
                 0 
                 0 
               
               
                 29 
                 S 
                 −1 
                 −1 
                 3 
                 −1 
                 −2 
                 −1 
                 −1 
                 −2 
                 0 
                 −1 
                 1 
                 −1 
                 0 
                 1 
                 −2 
                 1 
                 0 
                 0 
                 4 
                 −1 
               
               
                 30 
                 N 
                 −1 
                 −1 
                 4 
                 0 
                 −3 
                 −1 
                 −1 
                 3 
                 0 
                 −3 
                 −3 
                 −1 
                 −2 
                 0 
                 −3 
                 0 
                 −1 
                 −1 
                 4 
                 −3 
               
               
                 31 
                 T 
                 −1 
                 −2 
                 −1 
                 −2 
                 −2 
                 −1 
                 −2 
                 −2 
                 −2 
                 1 
                 −1 
                 −1 
                 −1 
                 −2 
                 5 
                 0 
                 3 
                 −3 
                 −2 
                 0 
               
               
                 32 
                 P 
                 −1 
                 0 
                 −2 
                 −1 
                 −3 
                 0 
                 −1 
                 −2 
                 −2 
                 −3 
                 −3 
                 2 
                 −2 
                 −4 
                 7 
                 −1 
                 −1 
                 −4 
                 −3 
                 −3 
               
               
                 33 
                 V 
                 −1 
                 −3 
                 −3 
                 −4 
                 −1 
                 −2 
                 −3 
                 −4 
                 −3 
                 2 
                 2 
                 −3 
                 1 
                 −1 
                 −3 
                 −2 
                 0 
                 −3 
                 −1 
                 4 
               
               
                 34 
                 L 
                 −2 
                 3 
                 −2 
                 −3 
                 −2 
                 −1 
                 −2 
                 −3 
                 0 
                 0 
                 2 
                 0 
                 1 
                 1 
                 −3 
                 −2 
                 −1 
                 0 
                 4 
                 −1 
               
               
                 35 
                 L 
                 −2 
                 −3 
                 −4 
                 −4 
                 −2 
                 −3 
                 −3 
                 −4 
                 −3 
                 3 
                 3 
                 −3 
                 1 
                 3 
                 −3 
                 −3 
                 −1 
                 −1 
                 1 
                 1 
               
               
                 36 
                 D 
                 −2 
                 −2 
                 1 
                 6 
                 −4 
                 0 
                 1 
                 −2 
                 −1 
                 −4 
                 −4 
                 −1 
                 −3 
                 −4 
                 −2 
                 0 
                 −1 
                 −5 
                 −3 
                 −4 
               
               
                   
               
            
           
         
       
     
     The command line of PSI-BLAST that was used is as follows: psiblast-db&lt;LOCAL NR database name&gt;-max_target_seqs 2500-in_msa&lt;MSA file in FAST format&gt;-out&lt;results output file&gt;-outfmt “7sskingdoms sscinames scomnames sseqid stitle evalue length pident”-out_pssm&lt;PSSM file output&gt;-out_ascii_pssm&lt;PSSM (ascii) output&gt;-num_iterations 6-num_threads 8 
     This PSI-BLAST search resulted in an initial 2515 hits. There were 787 hits with greater than 22% sequence identity to FutC. 396 hits were of greater than 275 amino acids in length. Additional analysis of the hits was performed, including sorting by percentage identity to FutC, comparing the sequences by BLAST to an existing α(1,2) fucosyltransferase inventory (of known α(1,2) fucosyltransferases, to eliminate known lactose-utilizing enzymes and duplicate hits), and manual annotation of hits to identify those originating from bacteria that naturally exist in the gastrointestinal tract. An annotated list of the novel α(1,2) fucosyltransferases identified by this screen are listed in Table 1. Table 1 provides the bacterial species from which the enzyme is found, the GenBank Accession Number, GI Identification Number, amino acid sequence, and % sequence identity to FutC. 
     Multiple sequence alignment of the 4 known α(1,2) FTs used for the PSI-BLAST query and 12 newly identified α(1,2) FTs is shown in  FIG.  4   . 
     Example 2: Validation of Novel α(1,2) FTs 
     To test for lactose-utilizing fucosyltransferase activity, the production of fucosylated oligosaccharides (i.e., 2′-FL) is evaluated in a host organism that expresses the candidate enzyme (i.e., syngene) and which contains both cytoplasmic GDP-fucose and lactose pools. The production of fucosylated oligosaccharides indicates that the candidate enzyme-encoding sequence functions as a lactose-utilizing α(1,2)fucosyltransferase. Of the identified hits, 12 novel α(1,2) fucosyltransferases were further analyzed for their functional capacity to produce 2′-fucosyllactose:  Prevotella melaninogenica  FutO,  Clostridium bolteae  FutP,  Clostridium bolteae+ 13 FutP,  Lachnospiraceae  sp. FutQ,  Methanosphaerula palustries  FutR,  Tannerella  sp. FutS,  Bacteroides  caccae FutU,  Butyrivibrio  FutV,  Prevotellaa  sp. FutW,  Parabacteroides johnsonii  FutX,  Akkermansia muciniphilia  FutY,  Salmonella enterica  FutZ, and  Bacteroides  sp. FutZA. 
     Syngenes were constructed comprising the 12 novel α(1,2) FTs in the configuration as follows: EcoRI-T7g10 RBS-syngene-XhoI.  FIG.  5 A  and  FIG.  5 B  show the syngene fragments after PCR assembly and gel-purification. 
     The candidate α(1,2) FTs (i.e., syngenes) were cloned by standard molecular biological techniques into an exemplary expression plasmid pEC2-(T7)-Fut syngene-rcsA-thyA. This plasmid utilizes the strong leftwards promoter of bacteriophage λ (termed P L ) to direct expression of the candidate genes (Sanger, F. et al. (1982). J Mol Biol 162, 729-773). The promoter is controllable, e.g., a trp-cI construct is stably integrated the into the  E. coli  host&#39;s genome (at the ampC locus), and control is implemented by adding tryptophan to the growth media. Gradual induction of protein expression is accomplished using a temperature sensitive cI repressor. Another similar control strategy (temperature independent expression system) has been described (Mieschendahl et al., 1986, Bio/Technology 4:802-808). The plasmid also carries the  E. coli  rcsA gene to up-regulate GDP-fucose synthesis, a critical precursor for the synthesis of fucosyl-linked oligosaccharides. In addition, the plasmid carries a β-lactamase (bla) gene for maintaining the plasmid in host strains by ampicillin selection (for convenience in the laboratory) and a native thyA (thymidylate synthase) gene as an alternative means of selection in thyA −  hosts. 
     The expression constructs were transformed into a host strain useful for the production of 2′-FL. The host strain used to test the different α(1,2) FT candidates incorporates all the above genetic modifications described above and has the following genotype: ΔampC::P trp   B cI, A(lacI-lacZ)::FRT, P lacIq lacY + , ΔwcaJ::FRT, thyA::Tn10, Δlon:(npt3, lacZ + ), ΔlacA 
     The  E. coli  strains harboring the different α(1,2) FT candidate expression plasmids were analyzed. Strains were grown in selective media (lacking thymidine) to early exponential phase. Lactose was then added to a final concentration of 0.5%, and tryptophan (200 μM) was added to induce expression of each candidate α(1,2) FT from the P L  promoter. At the end of the induction period (˜24 h) the culture supernatants and cells were harvested. Heat extracts were prepared from whole cells and the equivalent of 0.2OD 600  units of each strain analyzed for the presence of 2′-FL by thin layer chromatography (TLC), along with 2 μl of the corresponding clarified culture supernatant for each strain. 
       FIG.  6    shows the oligosaccharides produced by the α(1,2) FT-expressing bacteria, as determined by TLC analysis of the culture supernatant and extracts from the bacterial cells. 2′FL was produced by exogenous expression of WbgL (used as control), FutO, FutP, FutQ, FutR, FutS, FutU, FutW, FutX, FutZ, and FutZA. 
     Table 4 summarizes the fucosyltransferase activity for each candidate syngene as determined by the 2′FL synthesis screen described above. 11 of the 12 candidate α(1,2) FTs were found to have lactose-utilizing fucosyltransferase activity. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 2′FL synthesis screen results 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                 2′FL 
                 2′FL 
                   
                   
                   
               
               
                   
                   
                   
                 culture 
                 cell 
                   
                   
                   
               
               
                   
                   
                 24 h OD 
                 me- 
                 ex- 
                   
                   
                   
               
               
                   
                 Syngene 
                 (induced) 
                 dium 
                 tract 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 
                   Escherichia coli 
                 
                 WbgL 
                 9.58 
                 5 
                 5 
                 pG204 
                 pEC2-WbgL-rcsA-thyA 
                 E640 
               
               
                 
                   Prevotella  
                 
                 FutO 
                 12.2 
                 3 
                 2 
                 pG393 
                 pEC2-(T7)FutO-rcsA-thyA 
                 E985 
               
               
                 
                   melaninogenica 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Clostridium bolteae 
                 
                 FutP 
                 10.4 
                 1 
                 2 
                 pG394 
                 pEC2-(T7)FutP-rcsA-thyA 
                 E986 
               
               
                   Lachnospiraceae  sp .   
                 FutQ 
                 10.6 
                 3 
                 4 
                 pG395 
                 pEC2-(T7)FutQ-rcsA-thyA 
                 E987 
               
               
                 
                   Methanosphaerula  
                 
                 FutR 
                 11.9 
                 0 
                 1 
                 pG396 
                 pEC2-(T7)FutR-rcsA-thyA 
                 E988 
               
               
                 
                   palustris 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Tannerella  sp .   
                 FutS 
                 11.3 
                 2 
                 3 
                 pG397 
                 pEC2-(T7)FutS-rcsA-thyA 
                 E989 
               
               
                 
                   Bacteroides caccae 
                 
                 FutU 
                 12.1 
                 0 
                 2 
                 pG398 
                 pEC2-(T7)FutU-rcsA-thyA 
                 E990 
               
               
                 
                   Butyrivibrio 
                 
                 FutV 
                 11.3 
                 0 
                 1 
                 pG399 
                 pEC2-(T7)FutV-rcsA-thyA 
                 E991 
               
               
                   Prevotella  sp .   
                 FutW 
                 10.5 
                 3 
                 3 
                 pG400 
                 pEC2-(T7)FutW-rcsA-thyA 
                 E992 
               
               
                 
                   Parabacteroides  
                 
                 FutX 
                 10.7 
                 3 
                 5 
                 pG401 
                 pEC2-(T7)FutX-rcsA-thyA 
                 E993 
               
               
                 
                   johnsonii 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Akkermansia  
                 
                 FutY 
                 9.1 
                 0 
                 0 
                 pG402 
                 pEC2-(T7)FutY-rcsA-thyA 
                 E994 
               
               
                 
                   muciniphilia 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Salmonella enterica 
                 
                 FutZ 
                 11.0 
                 0 
                 3 
                 pG403 
                 pEC2-(T7)FutZ-rcsA-thyA 
                 E995 
               
               
                   Bacteroides  sp .   
                 FutZA 
                 9.9 
                 3 
                 3 
                 pG404 
                 pEC2-(T7)FutZA-rcsA-thyA 
                 E996 
               
               
                   
               
            
           
         
       
     
     Example 3: Characterization of Cultures Expressing Novel α(1,2) FTs 
     Further characterization of the bacterium expressing novel α(1,2) FTs FutO, FutQ, and FutX was performed. Specifically, proliferation rate and exogenous α(1,2) FT expression was examined. 
     Expression plasmids containing fucosyltransferases WbgL (plasmid pG204), FutN (plasmid pG217), and novel α(1,2) FTs FutO (plasmid pG393), FutQ (plasmid pG395), and FutX (pG401) were introduced into host bacterial strains. For example, the host strains utilized has the following genotype: ΔampC::P trp   B cI, A(lacI-lacZ)::FRT, P lacIq lacY + , ΔwcaJ::FRT, thyA::Tn10, Δlon:(npt3, lacZ + ), ΔlacA 
     Bacterial cultures expressing each exogenous fucosyltransferase were induced by addition of tryptophan (to induce expression of the exogenous fucosyltransferases) in the presence of lactose. Growth of the cultures was monitored by spectrophotometric readings at A600 at the following timepoints: 4 hours and 1 hour before induction, at the time of induction (time 0), and 3 hours, 7 hours, and 24 hours after induction. The results are shown in  FIG.  7   , and indicate that expression of the exogenous fucosyltransferase did not prevent cell proliferation. Furthermore, the growth curve for the bacterial cultures expressing the novel α(1,2) fucosyltransferases FutO, FutQ, and FutX is similar to those expressing the known α(1,2)FT enzymes WbgL and FutN. 
     Protein expression was also assessed for the bacterial cultures expressing each fucosyltransferase after induction. Cultures were induced as described previously, and protein lysates were prepared from the bacterial cultures at the time of induction (0 hours), 3 hours, 7 hours, and 24 hours after induction. The protein lysates were run on an SDS-PAGE gel and stained to examine the distribution of proteins at each time point. As shown in  FIG.  8   , induction at 7 hours and 24 hours showed increases in a protein band at around 20-28 kDa for bacterial cultures expressing exogenous FutN, FutO, and FutX. These results indicate that induction results in significant expression of the exogenous fucosyltransferases. 
     Finally, additional TLC analysis to assess the efficiency and yield of 2′FL production in bacterial cultures expressing novel α(1,2) FTs FutO, FutQ, and FutX compared to known fucosyltransferases WbgL and FutN. Cultures were induced at 7 hours and 24 hours, and run out on TLC.  FIG.  9 A  shows the level of 2′FL in the cell supernatant. The level of 2′FL found in the bacterial cells were also examined. As shown in  FIG.  9 B,  2   ′FL was produced in cell lysates from bacteria expressing the novel α(1,2) FTs FutO, FutQ, and FutX at 7 hours and 24 hours after induction. 
     Example 4: FutN Exhibits Increased Efficiency for Production of 2′FL 
     Fucosylated oligosaccharides produced by metabolically engineered  E. coli  cells to express  B. vulgatus  FutN was purified from culture broth post-fermentation. 
     Fermentation broth was harvested and cells were removed by sedimentation in a preparative centrifuge at 6000×g for 30 min. Each bioreactor run yields about 5-7 L of partially clarified supernatant. A column packed with coarse carbon (Calgon 12×40 TR) of ˜1000 ml volume (dimension 5 cm diameter×60 cm length) was equilibrated with 1 column volume (CV) of water and loaded with clarified culture supernatant at a flow rate of 40 ml/min. This column had a total capacity of about 120 g of sugar. Following loading and sugar capture, the column is washed with 1.5 CV of water, then was eluted with 2.5 CV of 50% ethanol or 25% isopropanol (lower concentrations of ethanol at this step (25-30%) may be sufficient for product elution.) This solvent elution step released about 95% of the total bound sugars on the column and a small portion of color bodies (caramelized sugars). A volume of 2.5 L of ethanol or isopropanol eluate from the capture column was rotary-evaporated at 56 C.° and a sugar syrup in water was generated. A column (GE Healthcare HiScale50/40, 5×40 cm, max pressure 20 bar) connected to a Biotage Isolera One FLASH Chromatography System was packed with 750 ml of a Darco Activated Carbon G60 (100-mesh): Celite 535 (coarse) 1:1 mixture (both column packings were obtained from Sigma). The column was equilibrated with 5 CV of water and loaded with sugar from step 3 (10-50 g, depending on the ratio of 2′-FL to contaminating lactose), using either a celite loading cartridge or direct injection. The column was connected to an evaporative light scattering (ELSD) detector to detect peaks of eluting sugars during the chromatography. A four-step gradient of isopropanol, ethanol or methanol was run in order to separate 2′-FL from monosaccharides (if present), lactose and color bodies. Fractions corresponding to sugar peaks were collected automatically in 120-ml bottles, pooled. 
     The results from two fermentation runs are shown in  FIG.  10 A  and  FIG.  10 B . The cultures were grown for 136 (run  36 B) or 112 hours (run  37 A), and the levels of 2′-FL produced was analyzed by TLC analysis. As shown in both  FIG.  10 A  and  FIG.  10 B , the 2′-fucosyllactose was produced at 40 hours of culture, and production continued to increase until the end point of the fermentation process. The yield of 2′-FL produced from run  36 B was 33 grams per liter. The yield of 2′-FL produced from run  37 A was 36.3 grams per liter. These results indicate that expression of exogenous FutN is suitable for high yield of 2′-fucosyllactose product. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Hits from PSI-BLAST multiple sequence alignment query for novel α(1,2)fucosyltransferases 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 % 
                   
                   
                   
               
               
                   
                   
                   
                   
                 iden- 
                   
                   
                 SEQ 
               
               
                   
                 Accession 
                   
                 Gene name 
                 tity 
                   
                   
                 ID 
               
               
                 Bacterium names 
                 No. 
                 GI No. 
                 [bacterium] 
                 FutC 
                 Alias 
                 SEQUENCE 
                 NO 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   Helicobacter   
                 AAD29869.1 
                 4808599 
                 alpha-1,2- 
                 98 
                 FutC 
                 MAFKVVQICGGLGNQMFQYAFAKSLQKHSNTPVLLDITSFDWSDRKMQLELFPINLPYASAKEIAIAKMQ 
                   1 
               
               
                 
                   pylori 
                 
                   
                   
                 fucosyl- 
                   
                   
                 HLPKLVRDALKCMGFDRVSQEIVFEYEPELLKPSRLTYFYGYFQDPRYFDAISPLIKQTFTLPPPPENNKNNN 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 KKEEEYHRKLSLILAAKNSVFVHIRRGDYVGIGCQLGIDYQKKALEYMAKRVPNMELFVFCEDLEFTQNLDLG 
                   
               
               
                   
                   
                   
                 [ Helicobacter   
                   
                   
                 YPFMDMTTRNKEEEAYWDMLLMQSCQHGIIANSTYSWWAAYLIENPEKIIIGPKHWLFGHENILCKEWVK 
                   
               
               
                   
                   
                   
                   pylori ] 
                   
                   
                 IESHFEVKSQKYNA 
                   
               
               
                   
               
               
                 
                   Helicobacter 
                 
                 YP_003517185.1 
                 291277413 
                 alpha-1,2- 
                 70.85 
                 FutL 
                 MDFKIVQVHGGLGNQMFQYAFAKSLQTHLNIPVLLDTTWFDYGNRELGLHLFPIDLQCASAQQIAAAHM 
                   2 
               
               
                   mustelae ;  
                   
                   
                 fucosyl- 
                   
                   
                 QNLPRLVRGALRRMGLGRVSKEIVFEYMPELFEPSRIAYFHGYFQDPRYFEDISPLIKQTFTLPHPTEHAEQY 
                   
               
               
                 
                   Helicobacter 
                 
                   
                   
                 transferase 
                   
                   
                 SRKLSQILAAKNSVFVHIRRGDYMRLGWQLDISYQLRAIAYMAKRVQNLELFLFCEDLEFVQNLDLGYPFVD 
                   
               
               
                   mustelae  12198 
                   
                   
                 [ Helicobacter   
                   
                   
                 MTTRDGAAHWDMMLMQSCKHGIITNSTYSWWAAYLIKNPEKIIIGPSHWIYGNENILCKDWVKIESQFET 
                   
               
               
                   
                   
                   
                 
                   mustelae 
                 
                   
                   
                 KS 
                   
               
               
                   
                   
                   
                 12198] 
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides ;  
                 YP_001300461.1 
                 150005717 
                 glycosyl 
                 24.83 
                 FutN 
                 MRLIKVTGGLGNQMFIYAFYLRMKKYYPKVRIDLSDMMHYKVHYGYEMHRVFNLPHTEFCINQPLKKVIEF 
                   3 
               
               
                 
                   Bacteroides 
                 
                   
                   
                 transferase 
                   
                   
                 LFFKKIYERKQAPNSLRAFEKKYFWPLLYFKGFYQSERFFADIKDEVRESFTFDKNKANSRSLNMLEILDKD 
                   
               
               
                   vulgatus   
                   
                   
                 family protein 
                   
                   
                 ENAVSLHIRRGDYLQPKHWATTGSVCQLPYYQNAIAEMSRRVASPSYYIFSDDIAWVKENLPLQNAVYIDWN 
                   
               
               
                 ATCC 8482;  
                   
                   
                 [ Bacteroides   
                   
                   
                 TDEDSWQDMMLMSHCKHHIICNSTFSWWGAWLNPNMDKTVIVPSRWFQHSEAPDIYPTGWIKVPVS 
                   
               
               
                   Bacteroides   
                   
                   
                   vulgatus  ATCC 
                   
                   
                   
                   
               
               
                 sp. 4_3_47FAA;  
                   
                   
                 8482] 
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 sp. 3_1_40A;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   vulgatus 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 PC510;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   vulgatus 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CL09T03C04;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   vulgatus 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 dnLKV7;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   vulgatus 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CAG: 6 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 Escherichia 
                 WP_021554465.1 
                 545259828 
                 protein 
                 23.13 
                 WbgL 
                 MSIIRLQGGLGNQLFQFSFGYALSKINGTPLYFDISHYAENDDHGGYRLNNLQIPEEYLQYYTPKINNIYKLLV 
                   4 
               
               
                   coli ;   
                   
                   
                 [ Escherichia   
                   
                   
                 RGSRLYPDIFLFLGFCNEFHAYGYDFEYIAQKWKSKKYIGYWQSEHFFHKHILDLKEFFIPKNVSEQANLLAAK 
                   
               
               
                 
                   Escherichia 
                 
                   
                   
                   coli ] 
                   
                   
                 ILESQSSLSIHIRRGDYIKNKTATLTHGVCSLEYYKKALNKIRDLAMIRDVFIFSDDIFWCKENIETLLSKKYN 
                   
               
               
                 
                   coli 
                 
                   
                   
                   
                   
                   
                 IYYSEDLSQEEDLWLMSLANHHIIANSSFSWWGAYLGSSASQIVIYPTPWYDITPKNTYIPIVNHWINVDKHS 
                   
               
               
                 UMEA 3065-1 
                   
                   
                   
                   
                   
                 SC 
                   
               
               
                   
               
               
                 
                   Helicobacter 
                 
                 WP_005219731.1 
                 491361813 
                 predicted 
                 36.79 
                 FutD 
                 MGDYKIVELTCGLGNQMFQYAFAKALQKHLQVPVLLDKTWYDTQDNSTQFSLDIFNVDLEYATNTQIEKA 
                   5 
               
               
                   bilis ;  
                   
                   
                 protein 
                   
                   
                 KARVSKLPGLLRKMFGLKKHNIAYSQSFDFHDEYLLPNDFTYFSGFFQNAKYLKGLEQELKSIFYYDSNNFSN 
                   
               
               
                   Helicobacter   
                   
                   
                 [ Helicobacter   
                   
                   
                 FGKQRLELILQAKNSIFIHIRRGDYCKIGWELGMDYYKRAIQYIMDRVEEPKFFIFGATDMSFTEQFQKNLGL 
                   
               
               
                 
                   bilis 
                 
                   
                   
                   bilis ] 
                   
                   
                 NENNSANLSEKTITQDNQHEDMFLMCYCKHAILANSSYSFWSAYLNNDANNIVIAPTPWLLDNDNIICDD 
                   
               
               
                 ATCC 43879 
                   
                   
                   
                   
                   
                 WIKISSK 
                   
               
               
                   
               
               
                   Escherichia   
                 AAO37698.1 
                 37788088 
                 fucosyl- 
                 25.94 
                 WbsJ 
                 MEVKIIGGLGNQMFQYATAFAIAKRTHQNLTVDISDAVKYKTHPLRLVELSCSSEFVKKAWPFEKYLFSEKIP 
                   6 
               
               
                 
                   coli 
                 
                   
                   
                 transferase 
                   
                   
                 HFMKKGMFRKHYVEKSLEYDPDIDTKSINKKIVGYFQTEKYFKEFRHELIKEFQPKTKFNSYQNELLNLIKEND 
                   
               
               
                   
                   
                   
                 [ Escherichia   
                   
                   
                 TCSLHIRRGDYVSSKIANETHGTCSEKYFERAIDYLMNKGVINKKTLLFIFSDDIKWCRENIFFNNQICFVQGD 
                   
               
               
                   
                   
                   
                   coli ] 
                   
                   
                 AYHVELDMLLMSKCKNNIISNSSFSWWAAWLNENKNKTVIAPSKWFKKDIKHDIIPESWVKL 
                   
               
               
                   
               
               
                   Vibrio   
                 BAA33632.1 
                 3721682 
                 probable beta- 
                 25.94 
                 WblA 
                 MIVMKISGGLGNQLFQYAVGRAIAIQYGVPLKLDVSAYKNYKLHNGYRLDQFNINADIANEDEIFHLKGSSN 
                   7 
               
               
                 
                   cholerae 
                 
                   
                   
                 D-galactoside  
                   
                   
                 RLSRILRRLGWLKKNTYYAEKQRTIYDVSVFMQAPRYLDGYWQNEQYFSQIRAVLLQELWPNQPLSINAQA 
                   
               
               
                   
                   
                   
                 2-alpha-L- 
                   
                   
                 HQIKIQQTHAVSIHVRRGDYLNHPEIGVLDIDYYKRAVDYIKEKIEAPVFFVFSNDVAWCKDNFNFIDSPVFI 
                   
               
               
                   
                   
                   
                 fucosyl 
                   
                   
                 EDTQTEIDDLMLMCQCQHNIVANSSFSWWAAWLNSNVDKIVIAPKTWMAENPKGYKWVPDSWREI 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                   
                   
               
               
                   
                   
                   
                 [ Vibrio   
                   
                   
                   
                   
               
               
                   
                   
                   
                   cholerae ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 YP_099118.1 
                 53713126 
                 alpha-1,2- 
                 24.58 
                 Bft2 
                 MIVSSLRGGLGNQMFIYAMVKAMALRNNVPFAFNLTTDFANDEVYKRKLLLSYFALDLPENKKLTFDFSYG 
                   8 
               
               
                   fragilis ;  
                   
                   
                 fucosyl- 
                   
                   
                 NYYRRLSRNLGCHILHPSYRYICEERPPHFESRLISSKITNAFLEGYWQSEKYFLDYKQEIKEDFVIQKKLEY 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 transferase 
                   
                   
                 TSYLELEEIKLLDKNAIMIGVRRYQESDVAPGGVLEDDYYKCAMDIMASKVTSPVFFCFSQDLEWVEKHLAGK 
                   
               
               
                   fragilis   
                   
                   
                 [ Bacteroides   
                   
                   
                 YPVRLISKKEDDSGTIDDMFLMMHFRNYIISNSSFYWWGAWLSKYDDKLVIAPGNFINKDSVPESWFKLNVR 
                   
               
               
                 NCTC 9343;  
                   
                   
                   fragilis   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 YCH46] 
                   
                   
                   
                   
               
               
                 
                   fragilis 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 YCH46;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   fragilis   
                   
                   
                   
                   
                   
                   
                   
               
               
                 HMW 615 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Escherichia 
                 
                 WP_001592236.1 
                 486356116 
                 protein 
                 24.25 
                 WbgN 
                 MSIVVARLAGGLGNQMFQYAKGYAESVERNSYLKLDLRGYKNYTLHGGFRLDKLNIDNTFVMSKKEMCIF 
                   9 
               
               
                   coli ;   
                   
                   
                 [ Escherichia   
                   
                   
                 PNFIVRAINKFPKLSLCSKRFESEQYSKKINGSMKGSVEFIGFWQNERYFLEHKEKLREIFTPININLDAKE 
                   
               
               
                 
                   Escherichia 
                 
                   
                   
                   coli ] 
                   
                   
                 LSDVIRCTNSVSVHIRRGDYVSNVEALKIHGLCTERYYIDSIRYLKERFNNLVFFVFSDDIEWCKKYKNEIF 
                   
               
               
                 
                   coli 
                 
                   
                   
                   
                   
                   
                 SRSDDVKFIEGNTQEVDMWLMSNAKYHIIANSSFSWWGAWLKNYDLGITIAPTPWFEREELNSFDPCPEKWV 
                   
               
               
                 KTE84 
                   
                   
                   
                   
                   
                 RIEK 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 YP_003814512.1 
                 302346214 
                 glycosyl- 
                 31.1 
                 FutO 
                 MKIVKILGGLGNQMFQYALYLSLQESFPKERVALDLSSFHGYHLHNGFELENIFSVTAQKASAADIMRIAYYY 
                  10 
               
               
                   melaninogenica ; 
                   
                   
                 transferase 
                   
                   
                 PNYLLWRIGKRFLPRRKGMCLESSSLRFDESVLRQEGNRYFDGYWQDERYFAAYREKVLKAFTFPAFKRAE 
                   
               
               
                 P revotella   
                   
                   
                 family 11 
                   
                   
                 NLSLLEKLDENSIALHVRRGDYVGNNLYQGICDLDYYRTAIEKMCAHVTPSLFCIFSNDITWCQQHLQPYLK 
                   
               
               
                   melaninogenica   
                   
                   
                 [ Prevotella   
                   
                   
                 APVVYVTWNTGVESYRDMQLMSCCAHNIIANSSFSWWGAWLNQNREKVVIAPKKWLNMEECHFTLPA 
                   
               
               
                 ATCC 25845 
                   
                   
                 
                   melaninogenica 
                 
                   
                   
                 SWIKI 
                   
               
               
                   
                   
                   
                 ATCC 25845] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Clostridium 
                 
                 WP_002570768.1 
                 488634090 
                 protein 
                 29.86 
                 FutP 
                 MFQYALYKAFEQKHIDVYADLAWYKNKSVKFELYNFGIKINVASEKDINRLSDCQADFVSRIRRKIFGKKKSF 
                  11 
               
               
                   bolteae ;  
                   
                   
                 [ Clostridium   
                   
                   
                 VSEKNDSCYENDILRMDNVYLSGYWQTEKYFSNTREKLLEDYSFALVNSQVSEWEDSIRNKNSVSIHIRRGD 
                   
               
               
                 
                   Clostridium 
                 
                   
                   
                   bolteae ] 
                   
                   
                 YLQGELYGGICTSLYYAEAIEYIKMRVPNAKFFVFSDDVEWVKQQEDFKGFVIVDRNEYSSALSDMYLMSLC 
                   
               
               
                 
                   bolteae 
                 
                   
                   
                   
                   
                   
                 KHNIIANSSFSWWAAWLNRNEEKIVIAPRRWLNGKCTPDIWCKKWIRI 
                   
               
               
                 90A9;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Clostridium 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   bolteae 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 90B3;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Clostridium 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   bolteae  90B8 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_009251343.1 
                 496545268 
                 protein 
                 29.25 
                 FutQ 
                 MVIVQLSGGLGNQMFEYALYLSLKAKGKEVKIDDVTCYEGPGTRPRQLDVFGITYDRASREELTEMTDASM 
                  12 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 DALSRVRRKLTGRRTKAYRERDINFDPLVMEKDPALLEGCFQSDKYFRDCEGRVREAYRFRGIESGAFPLPE 
                   
               
               
                 3_1_57FAA_CT1 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 DYLRLEKQIEDCQSVSVHIRRGDYLDESHGGLYTGICTEAYYKEAFARMERLVPGARFFLFSNDPEWTREHF 
                   
               
               
                   
                   
                   
                 3_1_57FAA_CT1] 
                   
                   
                 ESKNCVLVEGSTEDTGYMDLYLMSRCRHNIIANSSFSWWGAWLNENPEKKVIAPAKWLNGRECRDIYTER 
                   
               
               
                   
                   
                   
                   
                   
                   
                 MIRL 
                   
               
               
                   
               
               
                 
                   Methanosphaerula 
                 
                 YP_002467213.1 
                 219852781 
                 glycosyl 
                 28.52 
                 FutR 
                 MIIVRLKGGLGNQLSQYALGRKIAHLHNTELKLDTTWFTTISSDTPRTYRLNNYNIIGTIASAKEIQLIERG 
                  13 
               
               
                   palustris ;  
                   
                   
                 transferase 
                   
                   
                 RAQGRGYLLSKISDLLTPMYRRTYVRERMHTFDKAILTVPDNVYLDGYWQTEKYFKDIEEILRREVTLKDEP 
                   
               
               
                 
                   Methanosphaerula 
                 
                   
                   
                 family protein 
                   
                   
                 DSINLEMAERIQACHSVSLHVRRGDYVSNPTTQQFHGCCSIDYYNRAISLIEEKVDDPSFFIFSDDLPWAKE 
                   
               
               
                   palustris  E1-9c 
                   
                   
                 [ Methanosphaerula   
                   
                   
                 NLDIPGEKTFVAHNGPEKEYCDLWLMSLCQHHIIANSSFSWWGAWLGQDAEKMVIAPRRWALSESFDTSDII 
                   
               
               
                   
                   
                   
                   palustris  E1- 
                   
                   
                 PDSWITI 
                   
               
               
                   
                   
                   
                 9c] 
                   
                   
                   
                   
               
               
                   
               
               
                   Tannerella  sp. 
                 WP_021929367.1 
                 547187521 
                 glycosyl 
                 28.38 
                 FutS 
                 MVRIVEIIGGLGNQMFQYAFSLYLKNKSHIWDRLYVDIEAMKTYDRHYGLELEKVFNLSLCPISNRLHRNLQ 
                  14 
               
               
                 CAG: 118 
                   
                   
                 transferase 
                   
                   
                 KRSFAKHFVKSLYEHSECEFDEPVYRGLRPYRYYRGYWQNEGYFVDIEPMIREAFQFNVNILSKKTKAIASK 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 MRRELSVSIHVRRGDYENLPEAKAMHGGICSLDYYHKAIDFIRQRLDNNICFYLFSDDINWVEENLQLENRC 
                   
               
               
                   
                   
                   
                 [ Tannerella  sp. 
                   
                   
                 IIDWNQGEDSWQDMYLMSCCRHHIIANSSFSWWAAWLNPNKNKIVLTPNKWFNHTDAVGIVPKSWIKI 
                   
               
               
                   
                   
                   
                 CAG: 118] 
                   
                   
                 PVF 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_005675707.1 
                 491925845 
                 protein 
                 28.09 
                 FutU 
                 MKIVKILGGLGNQMFQYALFLSLKERFPHEQVMIDTSCFRNYPLHNGFEVDRIFAQKAPVASWRNILKVAY 
                  15 
               
               
                   caccae ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 PYPNYRFWKIGKYILPKRKTMCVERKNFSFDAAVLTRKGDCYYDGYWQHEEYFCDMKETIWEAFSFPEPV 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   caccae ] 
                   
                   
                 DGRNKEIGALLQASDSASLHVRRGDYVNHPLFRGICDLDYYKRAIHYMEERVNPQLYCVFSNDMAWCESH 
                   
               
               
                 caccae  
                   
                   
                   
                   
                   
                 LRALLPGKEVVYVDWNKGAESYVDMRLMSLCRHNIIANSSFSWWGAWLNRNPQKVVVAPERWMNSPI 
                   
               
               
                 ATCC43185 
                   
                   
                   
                   
                   
                 EDPVSDKWIKL 
                   
               
               
                   
               
               
                   Butyrivibrio  sp. 
                 WP_022772718.1 
                 551028636 
                 protein 
                 27.8 
                 FutV 
                 MIIIQLKGGLGNQMFQYALYKSLKKRGKEVKIDDKTGFVNDKLRIPVLSRWGVEYDRATDEEIINLTDSKMD 
                  16 
               
               
                 AE2015 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 LFSRIRRKLTGRKTFRIDEESGKFNPEILEKENAYLVGYWQCDKYFDDKDVVREIREAFEKKPQELMTDASS 
                   
               
               
                   
                   
                   
                 sp. AE2015] 
                   
                   
                 WSTLQQIECCESVSLHVRRTDYVDEEHIHIHNICTEKYYKNAIDRVRKQYPSAVFFIFTDDKEWCRDHFKGP 
                   
               
               
                   
                   
                   
                   
                   
                   
                 NFIWELEEGDGTDIAEMTLMSRCKHHIIANSSFSWWAAWLNDSPEKIVIAPQKWINNRDMDDIYTERMTKIAL 
                   
               
               
                   
               
               
                   Prevotella  sp. 
                 WP_022481266.1 
                 548264264 
                 uncharacterized 
                 27.4 
                 FutW 
                 MRLVKMIGGLGNQMFIYAFYLQMRKRFSNVRIDLTDMMHYNVHYGYELHKVFGLPRTEFCMNQPLKKVL 
                  17 
               
               
                 CAG: 891 
                   
                   
                 protein 
                   
                   
                 EFLFFRTIVERKQHGRMEPYTCQYVWPLVYFKGFYQSERYFSEVKDEVRECFTFNPALANRSSQQMMEQI 
                   
               
               
                   
                   
                   
                 [ Prevotella  sp. 
                   
                   
                 QNDPQAVSIHIRRGDYLNPKHYDTIGCICQLPYYKHAVSEIKKYVSNPHFYVFSEDLDWVKANLPLENAQYI 
                   
               
               
                   
                   
                   
                 CAG: 891] 
                   
                   
                 DWNKGADSWQDMMLMSCCKHHIICNSTFSWWAAWLNPSVEKTVIMPEQWTSRQDSVDFVASCGRW 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VRVKTE 
                   
               
               
                   
               
               
                 
                   Parabacteroides 
                 
                 WP_008155883.1 
                 495431188 
                 glycosyl 
                 26.69 
                 FutX 
                 MRLIKMIGGLGNQMFIYAFYLKMKHHYPDTNIDLSDMVHYKVHNGYEMNRIFDLSQTEFCINRTLKKILEFL 
                  18 
               
               
                   johnsonii ;  
                   
                   
                 transferase 
                   
                   
                 FFKKIYERRQDPSTLYPYEKRYFWPLLYFKGFYQSERFFFDIKDDVRKAFSFNLNIANPESLELLKQIEVDD 
                   
               
               
                   Parabacteroides   
                   
                   
                 [ Parabacteroides   
                   
                   
                 QAVSIHIRRGDYLLPRHWANTGSVCQLPYYKNAIAEMENRITGPSYYVFSDDISWVKENIPLKKAVYVTWNK 
                   
               
               
                 
                   johnsonii 
                 
                   
                   
                   johnsonii ] 
                   
                   
                 GEDSWQDMMLMSHCRHHIICNSTFSWWGAWLNPRKEKIVIAPCRWFQHKETPDMYPKEWIKVPIN 
                   
               
               
                 CL02T12C29 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Akkermansia 
                 
                 YP_001877555.1 
                 187735443 
                 glycosyl 
                 25.67 
                 FutY 
                 MRLFGGLGNQLFQYAFLFALSRQGGKARLETSSYEHDDKRVCELHHFRVSLPIEGGPPPWAFRKSRIPACLR 
                  19 
               
               
                   muciniphila ;  
                   
                   
                 transferase 
                   
                   
                 SLFAAPKYPHFREEKRHGFDPGLAAPPRRHTYFKGYFQTEQYFLHCREQLCREFRLKTPLTPENARILEDIRSC 
                   
               
               
                   Akkermansia   
                   
                   
                 family protein 
                   
                   
                 CSISLHIRRTDYLSNPYLSPPPLEYYLRSMAEMEGRLRAAGAPQESLRYFIFSDDIEWARQNLRPALPHVHVD 
                   
               
               
                 
                   muciniphila 
                 
                   
                   
                 [ Akkermansia   
                   
                   
                 INDGGTGYFDLELMRNCRHHIIANSTFSWWAAWLNEHAEKIVIAPRIWFNREEGDRYHTDDALIPGSWLRI 
                   
               
               
                 ATCCBAA-835 
                   
                   
                 
                   muciniphila 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 ATCCBAA-835] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Salmonella 
                 
                 WP_023214330.1 
                 555221695 
                 fucosyl- 
                 25.99 
                 FutZ 
                 MYSCLSGGLGNQMFQYAAAYILKQYFQSTTLVLDDSYYYSQPKRDTVRSLELNQFNISYDRFSFADEKEKIKL 
                  20 
               
               
                   enterica ;  
                   
                   
                 transferase 
                   
                   
                 LRKFKRNPFPKQISEILSIALFGKYALSDRAFYTFETIKNIDKACLFSFYQDADLLNKYKQLILPLFELRDDL 
                   
               
               
                 
                   Salmonella 
                 
                   
                   
                 [ Salmonella   
                   
                   
                 LDICKNLELYSLIQRSNNTTALHIRRGDYVTNQHAAKYHGVLDISYYNHAMEYVERERGKQNFIIFSDDVRWA 
                   
               
               
                   enterica  subsp. 
                   
                   
                   enterica ] 
                   
                   
                 QKAFLENDNCYVINNSDYDFSAIDMYLMSLCKNNIIANSTYSWWGAWLNKYEDKLVISPKQWFLGNNETSLRN 
                   
               
               
                   enterica   
                   
                   
                   
                   
                   
                 ASWITL 
                   
               
               
                 serovar 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Poona  str.  
                   
                   
                   
                   
                   
                   
                   
               
               
                 ATCCBAA- 
                   
                   
                   
                   
                   
                   
                   
               
               
                 1673 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_022161880.1 
                 547748823 
                 glycosyl- 
                 26.01 
                 FutZA 
                 MRLIKMTGGLGNQMFIYAFYLRMKKRYPKVRIDLSDMVHYHVHHGYEMHRVFNLPHTEFCINQPLKKVIE 
                  21 
               
               
                 CAG: 633 
                   
                   
                 transferase 
                   
                   
                 FLFFKKIYERKQDPNSLRAFEKKYLWPLLYFKGFYQSERFFADIKDEVRKAFTFDSSKVNARSAELLRRLDA 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 DANAVSLHIRRGDYLQPQHWATTGSVCQLPYYQNAIAEMNRRVAAPSYYVFSDDIAWVKENIPLQNAVYID 
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                 WNKGEESWQDMMLMSHCRHHIICNSTFSWWGAWLDPHEDKIVIVPNRWFQHCETPNIYPAGWVKVAIN 
                   
               
               
                   
                   
                   
                 sp. CAG: 633] 
                   
                   
                   
                   
               
               
                   
               
               
                   Clostridium  sp. 
                 WP_022247142.1 
                 547839506 
                 alpha-1 2- 
                 34.28 
                   
                 MEKIKIVKLQGGMGNQMFQYAFGKGLESKFGCKVLFDKINYDELQKTIINNTGKNAEGICVRKYELGIFNLN 
                  22 
               
               
                 CAG: 306 
                   
                   
                 fucosyl- 
                   
                   
                 IDFATAEQIQECIGEKLNKACYLPGFIRKIFNLSKNKTVSNRIFEKKYGEYDEEILKDYSLAYYDGYFQNPKY 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 FEDISDKIKKEFTLPEIKNHDIYNKKLLEKITQFENSVFIHVRRDDYLNINCEIDLDYYQKAVKYILKHIENP 
                   
               
               
                   
                   
                   
                 [ Clostridium   
                   
                   
                 KFFVFCAEDPDYIKNHFDIGYDFELVGENNKTQDTYYENMRLMMACKHAIIANSSYSWWAAWLSDYDNKIVIA 
                   
               
               
                   
                   
                   
                 sp. CAG: 306] 
                   
                   
                 PTPWLPGISNEIICKNWIQIKRGISNE 
                   
               
               
                   
               
               
                   Prevotella  sp.  
                 WP_009434595.1 
                 497004957 
                 protein 
                 32.11 
                   
                 MKIVKILGGLGNQMFQYALYLSLQESFPKERVALDLSCFNGYHLHNGFELERIFSLTAQKASAATIMRIAYYY 
                  23 
               
               
                 oral taxon 306;  
                   
                   
                 [ Prevotella  sp. 
                   
                   
                 PNYLLWRIGKRLLPRRKTMCLESSTFRYDESVLTREGNRYFDGYWQDERYFVACREKVLKAFTFPAFKRTEN 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                 oral taxon 306] 
                   
                   
                 LSLLRKLDKNSVAIHVRRGDYIGNQLYQGICDLDYYRAAIDKISTYVTPSVFCIFSNDIAWCQTHLQPYLKAP 
                   
               
               
                 sp. oral  
                   
                   
                   
                   
                   
                 VVYVTWNTGTESYRDMQLMSCCAHNIIANSSFSWWGAWLNQNNEKVVIAPKRWLNMDDCQFPLPASW 
                   
               
               
                 taxon 306 str. 
                   
                   
                   
                   
                   
                 VKI 
                   
               
               
                 F0472 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Brachyspira  sp. 
                 WP_021917109.1 
                 547139308 
                 glycosyl 
                 30.14 
                   
                 MQLVKLMGGLGNQMFQYAFAKALGDKNILFYGDYKKHSLRKVELNRFKCKAVYIPRELFKYLKFVFTKFDKI 
                  24 
               
               
                 CAG: 484 
                   
                   
                 transferase 
                   
                   
                 EYMRSGIYVPEYLNRDGNHIYIGFWQTEKYFKQIRPRLLKDFTPRKKLDRENAGIISKMQQINSVSVHIRRTD 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 YVDESHIYGDTNLDYYKRAIEYISSKIENPEFFFFSDDMAYVKEKFAGLKFPHSFIDINSGNNSYKDLILMKN 
                   
               
               
                   
                   
                   
                 [ Brachyspira  sp. 
                   
                   
                 CKHNIIANSTFSWWGAWLNENEEKIVIAPAKWFVTGENDKDIVPDEWIKL 
                   
               
               
                   
                   
                   
                 CAG: 484] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Thalassospira 
                 
                 WP_008889330.1 
                 496164823 
                 glycosyl 
                 30 
                   
                 MVIVKLLGGLGNQMFQYATGRAVASRLDVELLLDVSAFAHYDLRRYELDDWNITARLATSEELARSGVTAA 
                  25 
               
               
                   profundimaris ;  
                   
                   
                 transferase 
                   
                   
                 PPSFFDRIARFLRIDLPVNCFREASFTYDPRILEVSSPVYLDGYWQSERYFLDIEKKLRQEFQLKASIDANNH 
                   
               
               
                 
                   Thalassospira 
                 
                   
                   
                 family 11 
                   
                   
                 SFKKKIDGLGKQAVSLHVRRGDYVTNPQTASYHGVCSLDYYRAAVDYIAEHVSDPCFFVFSDDLEWVQTNLNI 
                   
               
               
                 
                   profundimaris 
                 
                   
                   
                 [ Thalassospira   
                   
                   
                 KQPIVLVDANGPDNGAADMALMMACRHHIIANSSFSWWGSWLNPLNDKIIVAPKKWFGRANHDTTDL 
                   
               
               
                 WP0211 
                   
                   
                   profundimaris ] 
                   
                   
                 VPDSWVRL 
                   
               
               
                   
               
               
                   Acetobacter  sp. 
                 WP_022078656.1 
                 547459369 
                 alpha-1 2- 
                 29.9 
                   
                 MAVSPQESKYSAHVSPDKPLRIVRLGGGLGNQMFQYAFGLAAGDVLWDNTSFLTNHYRSFDLGLYNISGD 
                  26 
               
               
                 CAG: 267 
                   
                   
                 fucosyl- 
                   
                   
                 FASNEQIKKCKNEIRFKNILPRSIRKKFNLGKFIYLKTNRVCERQINRYEPELLSKDGDVYYDGVFQTEKYFK 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 PLRERLLHDFTLTKPLDAANLDMLAKIRAADAVAVHIRRGDYLNPRSPFTYLDKDYFLNAMDYIGKRVDKPHF 
                   
               
               
                   
                   
                   
                 [ Acetobacter   
                   
                   
                 FIFSSDTDWVRTNIQTAYPQTIVEINDEKHGYFDLELMRNCRHNIIANSTFSWWGAWLNTNPDKIVVAPKQ 
                   
               
               
                   
                   
                   
                 sp. CAG: 267] 
                   
                   
                 WFRPDAAEYSGDIVPNDWIKL 
                   
               
               
                   
               
               
                 
                   Dysgonomonas 
                 
                 WP_006842165.1 
                 493896281 
                 protein 
                 29.9 
                   
                 MVTVLLSGGLGNQMFQYAAAKSLAIRLNTALSVDLYTFSKKTQATVRPYELGIFNIEDVVETSSLKAKAVIKA 
                  27 
               
               
                   mossii ;  
                   
                   
                 [ Dysgonomonas   
                   
                   
                 RPFIQRHRSFFQRFGVFTDTYAILYQPTFEALTGGVIMSGYFQNESYFKNISELLRKDFSFKYPLIGENKDVA 
                   
               
               
                 
                   Dysgonomonas 
                 
                   
                   
                   mossii ] 
                   
                   
                 GQISENQSVAVHIRRGDYLNKNSQSNFAILEKDYYEKAINYISAHVKNPEFYVFSEDFDWIKDNLNFKEFPVT 
                   
               
               
                   mossii   
                   
                   
                   
                   
                   
                 FIDWNKGKDSYIDMQLMSLCKHNIIANSSFSWWSAWLNNSEERKIVAPERWFVDEQKNELLDCFYPQGWI 
                   
               
               
                 DSM 22836 
                   
                   
                   
                   
                   
                 KI 
                   
               
               
                   
               
               
                 Clostridium sp.  
                 WP_021636924.1 
                 545396671 
                 glycosyl- 
                 29.83 
                   
                 &gt;gi|545396671|ref|WP_021636924.1|glycosyltransferase,family 11  
                  28 
               
               
                 KLE 1755 
                   
                   
                 transferase, 
                   
                   
                 [ Clostridium  sp. KLE 1755] 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 MIIIEISGGLGNQMFQYALGQKFISMGKEVKYDLSFYNDRVQTLRQFELDIFDLDCPVASNSELSRFG 
                   
               
               
                   
                   
                   
                 [ Clostridium   
                   
                   
                 KGNSLKSRLKQKLGWDKEKIYEENLDLGYQPRIFELDDIYLSGYWQSELYFKDIREQILRLYTFPIQLDYMN 
                   
               
               
                   
                   
                   
                 sp. KLE 1755] 
                   
                   
                 GVFLRKIENSNSVSIHIRRGDYLNENNLKIYGNICTLNYYNKALQIIAKKITNPIIFVFTNDIEWVRKELEI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PNMVIVDCNSGKLSYWDMYLMSKCKANIVANSSFSWWGAWLNKNENRIIISPKRWLNNHEQTSTLCDNWIRC 
                   
               
               
                   
                   
                   
                   
                   
                   
                 GDD 
                   
               
               
                   
               
               
                 
                   Gillisia 
                 
                 WP_006988068.1 
                 494045950 
                 alpha-1,2- 
                 29.28 
                   
                 MFISKNTVIIKLVGGLGNQMFQFAIAKIIAEKEKSEVLVDITFYTELTENTKKFPRHFSLGIFNSSFAIASKK 
                  29 
               
               
                   limnaea ;  
                   
                   
                 fucosyl- 
                   
                   
                 EIDYFTKLSNFNKFKKKLGLNYPTIFHESSFNFKAQVLELKAPIYLNGYFQSFRYFLGKEYVIRKIFKFPDEA 
                   
               
               
                 
                   Gillisia 
                 
                   
                   
                 transferase 
                   
                   
                 LDKDNDNIKRKIIGKTSVSLHIRRGDYVNNKKTQQFHGNCTIDYYQSAIAYLSSKLTDFNLIFFSDDIHWVRQ 
                   
               
               
                   limnaea   
                   
                   
                 [ Gillisia   
                   
                   
                 QFKNISNQKIYVSGNLNHNSWKDMYLMSLCDHNIIANSSFSWWGAWLNKNPEKIIIAPKRWFADTEQDKNSID 
                   
               
               
                 DSM 15749 
                   
                   
                   limnaea ] 
                   
                   
                 LIPSEWYRI 
                   
               
               
                   
               
               
                 
                   Methylotenera 
                 
                 YP_003048467.1 
                 253996403 
                 glycosyl 
                 29.19 
                   
                 MLVSRIIGGLGNQMFEYAAARAASLRISVQLKLDLSGFETYDLHAYGLNNFNIVEDVAKKDDYFIGAPESLLK 
                  30 
               
               
                   mobilis;   
                   
                   
                 transferase 
                   
                   
                 KIKKYLRGLIQLESFRESDLSFDSKVLELNDNTYLDGYWQCERYFIDFDKQIRQDFSFKFAPDALNQRYLELI 
                   
               
               
                 
                   Methylotenera 
                 
                   
                   
                 family protein 
                   
                   
                 DSVNAVSVHIRRGDYVSNSTTNEIHGVCDLDYYQRAAEFMRARIGPENLHFFVFSDDTDWVKENISFGSDTTF 
                   
               
               
                   mobilis  JLW8 
                   
                   
                 [ Methylotenera   
                   
                   
                 ISHNDAAKNYEDMRLMSACKHHIIANSSFSWWAAWLNPSKQKVVIAPRQWFKSTLLNSDDIVPASWVRL 
                   
               
               
                   
                   
                   
                   mobilis  JLW8] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Runella 
                 
                 YP_004658567.1 
                 338214504 
                 glycosyl 
                 29.14 
                   
                 MIIVKLSGGLGNQLFQYAFGRHLATVNQKELKLDTSALTKTSDWTNRSYALDAFNIRAQEATPEEIKALAGK 
                  31 
               
               
                   slithyformis ;  
                   
                   
                 transferase 
                   
                   
                 PNRLLQRVGRKVGITPIQYFQEPHFHFYSSALSIKSSHYLEGYWQSEKYFEAITPILREEFAFTISPSTHAQTI 
                   
               
               
                 
                   Runella 
                 
                   
                   
                 family protein 
                   
                   
                 KEKISNGTSVSIHLRRGDYVKTSKANRYLRPLTMDYYQKAIDYINQRVKNPNFFLFSDDIKWAKSQVTFPPTTH 
                   
               
               
                   slithyformis   
                   
                   
                 [ Runella   
                   
                   
                 FSTGTSAHEDLWLMTHCRHHIIANSTFSWWGAWLNQQPDKIVIAPQKWFSTERFDTKDLLPEPWIQL 
                   
               
               
                 DSM 19594 
                   
                   
                 
                   slithyformis 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 DSM 19594] 
                   
                   
                   
                   
               
               
                   
               
               
                   Pseudo - 
                 WP_002958454.1 
                 489048235 
                 alpha-1,2- 
                 29.1 
                   
                 MIKVKAIGGLGNQLFQYATARAIAEKRGDGVVVDMSDFSSYKTHPFCLNKFRCKATYESKPKLINKLLSNEKI 
                  32 
               
               
                 
                   alteromonas 
                 
                   
                   
                 fucosyl- 
                   
                   
                 RNLLQKLGFIKKYYFETQLPFNEDVLLNNSINYLTGYFQSEKYFLSIRECLLDELTLIEDLNIAETAVSKAIK 
                   
               
               
                   haloplanktis ;  
                   
                   
                 transferase 
                   
                   
                 NAKNSISIHIRRGDYVSNEGANKTHGVCDSDYFKKALNYFSERKLLDEHTELFIFSDDIEWCRNNLSFDYKMN 
                   
               
               
                   Pseudo -  
                   
                   
                 [ Pseudo-   
                   
                   
                 FVDGSSERPEVDMVLMSQCKHQVISNSTFSWWGAWLNKNDEKVVVAPKEWFKSTDLDSTDIVPNQWIKL 
                   
               
               
                 
                   alteromonas 
                 
                   
                   
                 
                   alteromonas 
                 
                   
                   
                   
                   
               
               
                 
                   haloplanktis 
                 
                   
                   
                   haloplanktis ] 
                   
                   
                   
                   
               
               
                 ANT/505 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 uncultured  
                 EKE06679.1 
                 406985989 
                 glycosyl 
                 28.67 
                   
                 MLTLKLKGGLGNQMFQYAASHNLAKNKKTKINFDLSFFSDIEVRDIKRDYLLDKFNISADISFDQKNSISGFR 
                  33 
               
               
                 bacterium 
                   
                   
                 transferase 
                   
                   
                 KFLVKVISKFFGEVFYYRLKFLSSKYLDGYFQSEKYFKNVEEDIRKDFTLKDEMGVEAKKIEQQIVNSKNSVSL 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 HIRRGDYVDDLKTNIYHGVCNLDYYKRSIKYLKENFGEINIFVFSDDIAWVKENLAFENLQFVSRPDIKDYEEL 
                   
               
               
                   
                   
                   
                 [uncultured 
                   
                   
                 MLMSKCEHNIIANSSFSWWGAWLNENKNKIIIAPKEWFQKFNINEKHIVPKSWIRL 
                   
               
               
                   
                   
                   
                 bacterium] 
                   
                   
                   
                   
               
               
                   
               
               
                   Clostridium  sp.  
                 WP_021636949.1 
                 545396696 
                 glycosyl- 
                 28.57 
                   
                 MVIVQLSGGLGNQMFEYALYLSLKAKGKVVKIDDITCYEGPGTRPKQLDVFGVSYERATKQELTEMTDSSL 
                  34 
               
               
                 KLE 1755 
                   
                   
                 transferase, 
                   
                   
                 DPVSRIRRKLTGRKTKAYREKDINFDPQVMERDPALLEGCFQSEKYFQDCREQVREAYRFRGIESGAYPLPE 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 AYRRLEKEIADCKSVSVHIRRGDYLEESHGGLYTGICTEQYYQEAFARMEKEVPGAKFFLFSNDPDWTREHF 
                   
               
               
                   
                   
                   
                 [ Clostridium   
                   
                   
                 KGENRILVEGSTEDTGYLDLYLMSKCKHNIIANSSFSWWGAWLNDNPEKKVTAPARWLNGRECRDIYTER 
                   
               
               
                   
                   
                   
                 sp. KLE 1755] 
                   
                   
                 MIRI 
                   
               
               
                   
               
               
                 
                   Francisella 
                 
                 WP_004287502.1 
                 490414974 
                 alpha-1,2- 
                 28.57 
                   
                 MKIIKIQGGLGNQMFQYAFYKSLKNNCIDCYVDIKNYDTYKLHYGFELNRIFKNIDLSFARKYHKKEVLGKLFS 
                  35 
               
               
                   philomiragia;   
                   
                   
                 fucosyl- 
                   
                   
                 IIPSKFIVKFNKNYILQKNFAFDKAYFEIDNCYLDGYWQSEKYFKKITKDIYDAFTFEPLDSINFEFLKNIQDY 
                   
               
               
                 
                   Francisella 
                 
                   
                   
                 transferase 
                   
                   
                 NLVSIHVRRGDYVNHPLHGGICDLEYYNKAISFIRSKVANVHFLVFSNDILWCKDNLKLDRVTYIDHNRWMDS 
                   
               
               
                 
                   philomiragia 
                 
                   
                   
                 [ Francisella   
                   
                   
                 YKDMHLMSLCKHNIIANSSFSWWGAWLNQNDDKIVIAPSKWFNDDKINQKDICPNSWVRI 
                   
               
               
                 subsp.  
                   
                   
                   philomiragia ] 
                   
                   
                   
                   
               
               
                 
                   philomiragia 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 ATCC 25015 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Pseudomonas 
                 
                 WP_017337316.1 
                 515906733 
                 protein 
                 28.52 
                   
                 MVIAHLIGGLGNQMFQYAAARALSSAKKEPLLLDTSSFESYTLHQGFELSKLFAGEMCIARDKDINHVLSW 
                  36 
               
               
                   fluorescens ;  
                   
                   
                 [ Pseudomonas   
                   
                   
                 QAFPRIRNFLHRPKLAFLRKASLIIEPSFHYWNGIQKAPADCYLMGYWQSERYFQDAAEEIRKDFTFKLNMS 
                   
               
               
                 
                   Pseudomonas 
                 
                   
                   
                   fluorescens ] 
                   
                   
                 PQNIATADQILNTNAISLHVRRGDYVNNSVYAACTVEYYQAAIQLLSKRVDAPTFFVFSDDIDWVKNNLNIG 
                   
               
               
                 
                   fluorescens 
                 
                   
                   
                   
                   
                   
                 FPHCYVNHNKGSESYNDMRLMSMCQHNIIANSSFSWWGAWLNSNADKIVVAPKQWFINNTNVNDLFP 
                   
               
               
                 NCIMB 11764 
                   
                   
                   
                   
                   
                 PAWVTL 
                   
               
               
                   
               
               
                   Herbaspirillum   
                 WP_008117381.1 
                 495392680 
                 glycosyl 
                 28.48 
                   
                 MIATRLIGGLGNQMFQYAAGRALALRVGSPLLLDVSGFANYELRRYELDGFRIDATAASAQQLARLGVNAT 
                  37 
               
               
                 sp. YR522 
                   
                   
                 transferase 
                   
                   
                 PGTSLLARVLRKVWPQPADRILREASFTYDARIEQASAPVYLDGYWQSERYFARIRQHLLDEFTLKGDWGS 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 DNAAMAAQIATAGAGAVSLHVRRGDYVSNAHTAQYHGVCSLDYYRDAVAHIGGRVEAPHFFVFSDDHE 
                   
               
               
                   
                   
                   
                 [ Herbaspirillum   
                   
                   
                 WVRENLQIGHPATFVQINSADHGIYDMMLMKSCRHHIIANSSFSWWGAWLNPAEDKIVVAPQRWFKD 
                   
               
               
                   
                   
                   
                 sp. YR522] 
                   
                   
                 ATNDTRDLIPAAWVRL 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_008822166.1 
                 496097659 
                 rotein 
                 28.43 
                   
                 MKIVKILGGLGNQMFQYALYLSLKETFPQENVTVDLSCFHGYHLHNGFEIARIFSLHPDKATVMEILRIAYYY 
                  38 
               
               
                   histicola ;  
                   
                   
                 [ Prevotella   
                   
                   
                 PNYFFWQIGKRVLPQRKTMCTESTKLLFDKSVLQREGDRYFDGYWQDERYFIDCRRTILNTFKFPPFTDDN 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                   histicola ] 
                   
                   
                 NLALLKKMDTNSVSIHVRRGDYVGNKLYQGICDLNYYREAIMKISSYISPSMFCVFSNDIEWCRDNLESFIKA 
                   
               
               
                   histicola   
                   
                   
                   
                   
                   
                 PIYYVDWNSGTESYRDMQLMSCCGHNIIANSSFSWWGAWLNQNSSKIVIAPKRWINLKNCGFMLPSRW 
                   
               
               
                 F0411 
                   
                   
                   
                   
                   
                 VKI 
                   
               
               
                   
               
               
                   Flavobacterium   
                 WP_017494954.1 
                 516064371 
                 protein 
                 28.42 
                   
                 MIVVQLIGGLGNQLFQYAAAKALALQTKQKFSLDVSQFESYKLHNYALNHFNVISKNYKKPNRYLRKIKSFY 
                  39 
               
               
                 sp. WG21 
                   
                   
                 [ Flavobacterium   
                   
                   
                 QKNVFYKEVDFGYNPDLIHLKGGIIFLEGYFQSEKYFIKYEKEIREDFELRTPLKKETKAAIAKIESVNSVSI 
                   
               
               
                   
                   
                   
                 sp. WG21] 
                   
                   
                 HIRRGDYINNPLHNTSKEEYYNKALEIVENKINNPVYFVFSDDMEWVKANFSTKQETIFIDFNDASTNFEDLK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 LMTSCKHNIIANSSFSWWGGWLNKNPDKIVIAPKRWFNDDSINTNDIIPTNWVKI 
                   
               
               
                   
               
               
                 
                   Polaribacter 
                 
                 WP_018944517.1 
                 517774309 
                 protein 
                 28.42 
                   
                 MIIVRIVGGLGNQMFQYAYAKALQQKGYQVKIDITKFKKYNLHGGYQLDQFKIDLETSSPIANVLCRIGLRRS 
                  40 
               
               
                 franzmannii 
                   
                   
                 [ Polaribacter   
                   
                   
                 VKEKSLLFDEKFLEIPQREYIKGYFQTEKYFSSITPILRKQFIVQKELCNTTLRYLKEITIQKNACSLHIRRG 
                   
               
               
                   
                   
                   
                   franzmannii ] 
                   
                   
                 DYISDEKANSVHGTCDLPYYKKSIKRIQDEYKDAHFFIFSDDISWAKKNLTNKNTTFIEHIVMPHEDMHLMSL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 CKHNITANSSFSWWGAWLNQHENKTVIAPKNWFVNRENEVACANWIQL 
                   
               
               
                   
               
               
                   Polaribacter   
                 YP_007670847.1 
                 472321325 
                 glycosyl 
                 28.42 
                   
                 MVVVRILGGLGNQMFQYAYAKSLAEKGYEVQIDISKFKSYKLHGGYHLDKFRIDLETANSSSAFLSKIGLKKT 
                  41 
               
               
                 sp. MED152 
                   
                   
                 transferase 
                   
                   
                 IKEPNLLFHKDLLKVNNNAFIKGYFQAEQYFSDIREILINQFKIKKELAKSTLAIKNQIELLKTTCSLHVRRG 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 DYISDKKANKVHGTCDLDYYSSAIEHISKQNSNVHFFVFSDDIAWVKDNLNITNATYIDHNVIPHEDMYLMTL 
                   
               
               
                   
                   
                   
                 [ Polaribacter   
                   
                   
                 CNHNITANSSFSWWGAWLNQNPDKIVIAPKNWFVDKENEVACKSWITL 
                   
               
               
                   
                   
                   
                 sp. MED152] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Methanococcus 
                 
                 YP_001329558.1 
                 150402264 
                 glycosyl 
                 28.19 
                   
                 MKIIQLKGGLGNQMFQYALYKSLKKRGQEVLLDISWYLKNNAHNGYELEWVFGLSPEYASIRQCFKLGDIPI 
                  42 
               
               
                   maripaludis ;  
                   
                   
                 transferase 
                   
                   
                 NLIYNVKRKVFPKKTHFFEKSNFNYDNNVFEVTNGYFEGYWQNENYFKNFRSEILNDFSFKNIDKRNAEFSE 
                   
               
               
                   Methanococcus   
                   
                   
                 family protein 
                   
                   
                 YLKSINSVSVHVRRGDYVTNQKALNVHGNICNLEYYNKAINLANNNLKNPKFVIFSDDITWCKSNLGIDDPV 
                   
               
               
                 
                   maripaludis 
                 
                   
                   
                 [ Methanococcus   
                   
                   
                 YVDWNTGPYSYQDMYLMSNCKNNIIANSSFSWWGAWLNQNTEKKVFSPKKWVNDRNNVNIVPNGWI 
                   
               
               
                 C7 
                   
                   
                   maripaludis  C7] 
                   
                   
                 KIK 
                   
               
               
                   
               
               
                   Gallionella   
                 WP_018293379.1 
                 517104561 
                 protein 
                 28.15 
                   
                 MIIAHIIGGLGNQMFQYAAGRALSLARGVPFKLDISGFEGYDLHQGFELQRVFNCAAGIASEAEVRDSLGW 
                  43 
               
               
                 sp. SCGC 
                   
                   
                 [ Gallionella   
                   
                   
                 QFSSPIRRIVARPSLAVLRRSTFVVEPHFHYWAGIKQVPDNCYLAGYWQSEQYFQSHAAVIRTDFAFKPPLS 
                   
               
               
                 AAA018-N21 
                   
                   
                 sp. SCGC  
                   
                   
                 GQNSKLAMQIAQGNAVSLHIRRGDYANNPKTTATHGLCSLDYYRAAIQHIAERVQSPHFFIFSDDIAWVKS 
                   
               
               
                   
                   
                   
                 AAA018-N21] 
                   
                   
                 NLAINFPHQYVDHNQGTESYNDMRLMSLCQHNIIANSSFSWWGAWLNTNAHKIVIAPKQWFANTTHVA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DLIPSSWERL 
                   
               
               
                   
               
               
                 
                   Azospira 
                 
                 YP_005026324.1 
                 372486759 
                 Glycosyl 
                 28.04 
                   
                 MQSPACIAGARAWWVGYGMAEAMQPVVVGLSGGLGNQMFQYAAGRALAHRLGHPLSLDLSWFQGR 
                  44 
               
               
                   oryzae ;  
                   
                   
                 transferase 
                   
                   
                 GDRHFALAPFHIAASLERAWPRLPPAMQAQLSRLSRRWAPRIMGAPVFREPHFHYVPAFAALAAPVFLEG 
                   
               
               
                 
                   Dechlorosoma 
                 
                   
                   
                 family 11 
                   
                   
                 YWQSERYFRELREPLLQDFSLRQPLPASCQPILAAIGNSDAICVHVRRGDYLSNPVAAKVHGVCPVDYYQQ 
                   
               
               
                   suillum  PS 
                   
                   
                 [ Dechlorosoma   
                   
                   
                 GVAELSASLARPHCFVFSDDPEWVRGSLAFPCPMTVVDVNGPAEAHFDLALMAACQHFVIANSSLSWW 
                   
               
               
                   
                   
                   
                   suillum  PS] 
                   
                   
                 GAWLGQAAGKRVIAPSRWFLTSDKDARDLLPPSWERR 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_018463017.1 
                 517274199 
                 protein 
                 28 
                   
                 MKIVKIIGGLGNQMFQYALAMALNKNFTDEEVKLDIHCFNGYTKHQGFEIDRVFGNEFELASYRDVAKVAY 
                  45 
               
               
                 
                   paludivivens 
                 
                   
                   
                 [ Prevotella   
                   
                   
                 PYFNFQLWRIGSRIFPDRRHMISEDTSFKIMPEVITSHNYKYYDGYWQHEEYFKNIHDEILDAFKFPKFQDER 
                   
               
               
                   
                   
                   
                   paludivivens ] 
                   
                   
                 NKALAERLSDSNSISIHIRRGDYLNDELFKGTCGIEYYKKAIEEINERTVPTLFCVFSNDIHWCKENIEPLLN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 GKETIYVDWNTGSDNYRDMQLMTKCKHNIIANSSFSWWGAWLNNTKDKIVIAPRIWYNTKEKVSPVANSWIKL 
                   
               
               
                   
               
               
                 
                   Gramella 
                 
                 YP_860609.1 
                 120434923 
                 alpha-1,2- 
                 27.96 
                   
                 MSNKNPVIVEIMGGLGNQMFQFAVAKLLAEKNSSVLLVDTNFYKEISQNLKDFPRYFSLGIFDISYKMGTEN 
                  46 
               
               
                   forsetii ;  
                   
                   
                 fucosyl- 
                   
                   
                 GMVNFKNLSFKNRVSRKLGLNYPKIFKEKSYRFDADLFNKKTPIYLKGYFQSYKYFIGVESKIRQWFEFPYE 
                   
               
               
                 
                   Gramella 
                 
                   
                   
                 transferase 
                   
                   
                 NLGVGNEEIKSKILEKTSVSVHIRRGDYVENKKTKEFHGNCSLEYYKNAITYFLDIVKEFNIVFFSDDISWV 
                   
               
               
                 
                   forsetii 
                 
                   
                   
                 [ Gramella   
                   
                   
                 RDEFKDLPNEKVFVTGNLHENSWKDMYLMSLCDHNIIANSSFSWWAAWLNNNSEKNVIAPKKWFADIDQEQK 
                   
               
               
                 KT0803 
                   
                   
                 
                   forsetii 
                 
                   
                   
                 SLDLLPPSWIRM 
                   
               
               
                   
                   
                   
                 KT0803] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Mariprofundus 
                 
                 WP_009849029.1 
                 497534831 
                 alpha-1,2- 
                 27.92 
                   
                 MIIVQFTGGLGNQMFQYALGRRLSLLHDVELKFDLSFYQHDILRDFMLDRFQVNGQVATEKEIEAYTNTPIF 
                  47 
               
               
                   ferrooxydans ;  
                   
                   
                 fucosyl- 
                   
                   
                 ALDRPLLDRLVRWGLYRGIVSVSDEPPGKQALMVYNSRVLQAPRNTYVQGYWQSEKYFMPIRQKLLDDFS 
                   
               
               
                 
                   Mariprofundus 
                 
                   
                   
                 transferase 
                   
                   
                 LVDKADQANGAMLEKIRQCHSVSLHVRRGDYVSNPLTNHSHGTCGLEYYEKAIALIGSKVDDPHFFVFSDD 
                   
               
               
                   ferrooxydans   
                   
                   
                 [ Mariprofundus   
                   
                   
                 PEWTRDHLKCRFPMTYVTCNSADSCEWDMELMRHCRDHIIANSSFSWWGAWLNMNPDKVVVAPAA 
                   
               
               
                 PV-1 
                   
                   
                   ferrooxydans ] 
                   
                   
                 WFNNFSADTSDLIPDSWVRI 
                   
               
               
                   
               
               
                 
                   Bacillus 
                 
                 WP_002174293.1 
                 488102896 
                 protein 
                 27.91 
                   
                 MKIIQVSSGLGNQMFQYALYKKISLNDNDVFLDSSTSYMMYKNQHNGYELERIFHIKPRHAGKEIIDNLSDL 
                  48 
               
               
                   cereus ;  
                   
                   
                 [ Bacillus   
                   
                   
                 DSELISRIRRKLFGAKKSMYVELKEFEYDPIIFEKKETYFKGYWQNYNYFKDIEQELRKDFVFTEKLDKRNEK 
                   
               
               
                 
                   Bacillus 
                 
                   
                   
                   cereus ] 
                   
                   
                 LANEIRNKNSVSIHIRRGDYYLNKVYEEKFGNIANLEYYLKAINLVKKKIEDPKFYIFSDDIDWAQKNINLTN 
                   
               
               
                   cereus  VD107 
                   
                   
                   
                   
                   
                 DVVYISHNQGNESYKDMQLMSLCKHNIIANSTFSWWGAFLNNNDDKIVVAPKKWINIKGLEKVELFPENWITY 
                   
               
               
                   
               
               
                   Firmicutes   
                 WP_022352106.1 
                 547951299 
                 protein 
                 27.81 
                   
                 MIIIRMTGGLGNQMFQYALYLKLRAMGKEVKMDDFTEYEGREARPLSLWAFGIEYDRASREELCRMTDGF 
                  49 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Firmicutes   
                   
                   
                 LDPVSRIRRKLFGRKSLEYMEKDCNFDPEILNRDPAYLTGYFQSEKYFADIEEEVRQAFRFSERIWEGIPSQL 
                   
               
               
                 CAG: 534 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 LERIRSYEQQIKTTMAVSVHIRRGDYLQNEEAYGGICFERYYKTAIEYVKKRQQDASFFVFTNDPDYAGEWIL 
                   
               
               
                   
                   
                   
                 CAG: 534] 
                   
                   
                 KNFGQEKERFVLIEGTQEENGYLDLYLMSLCRHHILANSSFSWWGAYLNPSREKMVIVPHKWFGNQECRD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 IYMENMIRIAKEQS 
                   
               
               
                   
               
               
                 
                   Sideroxydans 
                 
                 YP_003525501.1 
                 291615344 
                 glycosyl- 
                 27.81 
                   
                 MVISNIIGGLGNQMFQYAAARALSLKLEVPLKLDISGFTNYALHQGFELDRIFGCKIEIASEADVHEILGWQS 
                  50 
               
               
                   lithotrophicus ; 
                   
                   
                 transferase 
                   
                   
                 ASGIRRVVSRPGMSIFRRKGFVVEPHFSYWNGIRKITGDCYLAGYWQSEKYFLDAAVEIRKDFSFKLPLDSH 
                   
               
               
                 
                   Sideroxydans 
                 
                   
                   
                 family 11 
                   
                   
                 NAELAEKIDQENAVSLHIRRGDYANNPLTAATHGLCSLDYYRKSIKHIAGQVRNPYFFVFSDDIAWVKDNLEI 
                   
               
               
                 
                   lithotrophicus 
                 
                   
                   
                 [ Sideroxydans   
                   
                   
                 EFPSQYVDYNHGSMSFNDMRLMSLCKHHIIANSSFSWWGAWLNPNPEKVVIAPERWFANRTDVQDLLP 
                   
               
               
                 ES-1 
                   
                   
                 
                   lithotrophicus 
                 
                   
                   
                 PGWVKL 
                   
               
               
                   
                   
                   
                 ES-1] 
                   
                   
                   
                   
               
               
                   
               
               
                 zeta  
                 WP_018281578.1 
                 517092760 
                 protein [zeta 
                 27.81 
                   
                 MIVSQIIGGLGNQMFQYATGRALSHRLHDTFFLDLDGFSGYQLHQGFELSNVFQCEVNVATRSQMQALLG 
                  51 
               
               
                 proteobacterium 
                   
                   
                 proteo- 
                   
                   
                 WRSFSSVRRLLMKRSLKWARGHRVMIEPHFHYWSRFAEINEGCYLSGYWQSERYFKPIENIIRQDFKFNHL 
                   
               
               
                 SCGC 
                   
                   
                 bacterium   
                   
                   
                 LKGVNLDLAQQMTEVNSVSLHVRRGDYASDANTNHTHGLCPLDYYRDAILYIAQNTVAPSFFIFSDDIEWC 
                   
               
               
                 AB-137-C09 
                   
                   
                 SCGC 
                   
                   
                 REHLKLSFPATYIDHNKGSNSYCDMQLMSLCHHHIIANSSFSWWGAWLNTRLDKIVIAPKQWFANGNRT 
                   
               
               
                   
                   
                   
                 AB-137-C09] 
                   
                   
                 DDLIPAEWLVM 
                   
               
               
                   
               
               
                 
                   Pedobacter 
                 
                 YP_003090434.1 
                 255530062 
                 glycosyl 
                 27.8 
                   
                 MKIIRFLGGLGNQMFQYAFYKSLQHRFPHVKADLQGYQEYTLHNGFELEHIFNIKVNSVSSFTSDLFYNKK 
                  52 
               
               
                   heparinus ;  
                   
                   
                 transferase 
                   
                   
                 WLYRKLRRILNLRNTYIEEKKLFSFDPSLLNNPKSAYYWGYWQNFQYFEHIADDLRKDFQFRAPLSAQNQEV 
                   
               
               
                 
                   Pedobacter 
                 
                   
                   
                 family protein 
                   
                   
                 LDQTKLSNSISLHIRRGDYIKDPLLGGLCGPEYYQTAINYITSKVNAARFFIFSDDIDWCIANLKLQDCSFIS 
                   
               
               
                 
                   heparinus 
                 
                   
                   
                 [ Pedobacter   
                   
                   
                 WNKGTSSYIDMQLMSSCKHHIVANSSFSWWAAWLNPNPDKIVIAPEKWTNDKDINVRMSFPQGWISL 
                   
               
               
                 DSM 2366 
                   
                   
                   heparinus   
                   
                   
                   
                   
               
               
                   
                   
                   
                 DSM 2366] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Methylophilus 
                 
                 WP_018985060.1 
                 517814852 
                 protein 
                 27.78 
                   
                 MFQYAMGLSLAENNQTPLKLDLSQFTDYKLHNGFELSKVFNCSAETASVTQIETLLGICKYSFIRRILKNTYL 
                  53 
               
               
                 methylotrophus 
                   
                   
                 [ Methylophilus   
                   
                   
                 KNLRPAQYVVEPFFGYWDGVNFLGDNVYLEGYWQSQKYFIDYESTIRTHFTFKNILSGENLKLSDRIKGSNSV 
                   
               
               
                   
                   
                   
                   methylotrophus ] 
                   
                   
                 SLHIRRGDYVTNKNNAFIGTCSLIYYQNAIEYFSTKIADPIFFIFSDDITWAKSNLRLANEHYFVGHNQGEDS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 HFDMQLMSLCKHHIIANSSFSWWGAWLNPSKDKIIIAPKKWFASGLNDQDLVPKDWLRI 
                   
               
               
                   
               
               
                 
                   Rhodobacterales 
                 
                 WP_008033953.1 
                 495309205 
                 alpha-1,2- 
                 27.7 
                   
                 MIYTRIRGGLGNQLFQYSAARSLADYLNVSLGLDTREFDENSPYKMSLNHFNIRADLNPPDLIKHKKDGKIA 
                  54 
               
               
                   bacterium   
                   
                   
                 fucosyl- 
                   
                   
                 YIIDHIKGNQKKVYKEPFLSFDKNLFSNVDGTYLKGYWQSEKYFLRNRKNILSDINLIKKTDKFNTINLKEIK 
                   
               
               
                 HTCC2255 
                   
                   
                 transferase 
                   
                   
                 KSTSISLHIRRGDYLSNESYNETHGICSLSYYTDAVEYIKNRLGENIKVFAFSDDPDWVLENLKLSVDIKIIN 
                   
               
               
                   
                   
                   
                 [ Rhodobacterales   
                   
                   
                 NNTSANSFEDLRLMLNCDHNIIANSSFSWWGAWLNQNPEKIVISPKKWYNKKQLQNADIVPSSWLKY 
                   
               
               
                   
                   
                   
                 
                   bacterium 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 HTCC2255] 
                   
                   
                   
                   
               
               
                   
               
               
                   Spirulina   
                 WP_017302658.1 
                 515872075 
                 protein 
                 27.69 
                   
                 MAKIIARIRGGIGNQIFIYAAARRLELINNAELVLDSVSGFVHDLQYRQHYQLDHFHIPCRKATPAERFEPFSR 
                  55 
               
               
                 
                   subsalsa 
                 
                   
                   
                 [ Spirulina   
                   
                   
                 VRRYLKRQLNQRLPFEQRRYVIQESIDFDPRLIEFKPRGTVHLEGYWQSEDYFKDIEATIRQDLQIQPPTDPT 
                   
               
               
                   
                   
                   
                   subsalsa ] 
                   
                   
                 NLAIVQHIHQHTSVAVHIRFFDQPNADTMNNAPSDYYHRAVEAMETFVPGAHYYLFSDQPEAAKSRIPLP 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DERVTLVNHNRGNKLAYADLWLMTQCQHFIIANSTFSWWGAWLAENQKKQVIAPGFEKREGVSWWGF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KGLLPKQWIKL 
                   
               
               
                   
               
               
                   Vibrio   
                 WP_010433911.1 
                 498119755 
                 glycosyl 
                 27.67 
                   
                 MVIVKITGGLGNQLFQYATGSALANKLSCELVLDLSFYPTQTLRKYELAKFNINARVATDREIFLAGGGNDFF 
                  56 
               
               
                 
                   cyclitrophicus 
                 
                   
                   
                 transferase 
                   
                   
                 SKALKKLGLTSIIFPEYIKEQESIKYVGKIDLCKSGAYLDGYWQNPLYFSQNKIELTREFLPRAQLSPSALAW 
                   
               
               
                   
                   
                   
                 family 11  
                   
                   
                 KDHISQASNSVSLHVRRGDYVENAHTNNIHGTCSLEYYQHAIEKIRSEVHNPVFFVFSDDIEWCKLNLSSLAE 
                   
               
               
                   
                   
                   
                 [ Vibrio   
                   
                   
                 VEFVDNTTSAIDDLMLMRQCKHSIIANSTFSWWGAWLKLDGLVIAPRNWFSSASRNLKGIYPKEWHIL 
                   
               
               
                   
                   
                   
                   cyclitrophicus ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_022783177.1 
                 551039510 
                 protein 
                 27.65 
                   
                 MRSVVDIKGGYGNQLFCYSFGYAVSKETGSELIIDTSMLDMNNVKDRNYQLGVLGITYDSHISYKYGKDFLS 
                  57 
               
               
                   bacterium   
                   
                   
                 
                   [ 
                   Lachnospiraceae 
                 
                   
                   
                 RKTGLNRLRKKSAIGFGTVVFKEKEQYVYDPSVFEIKRDTYFDGFWQSSRYFEKYSDDLRKMLKPKKISNAAE 
                   
               
               
                 NK4A179 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 KLAEDARDCLSVSVHIRRGDYVSLGWTLKDDYYIKALDIIKERYGSEPVFFVFSDNKKYADDFFSAAGLKYRL 
                   
               
               
                   
                   
                   
                 NK4A179] 
                   
                   
                 MDYETDDAVRDDMFLMSRCSHNIMANSSYSWWGAFLNDNKDKTVICPETGVWGGDFYPEGWMKVTA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SSGK 
                   
               
               
                   
               
               
                 uncultured  
                 EKE02186.1 
                 406980610 
                 glycosyl 
                 27.57 
                   
                 MIIVNLYGGLGNQMFQYALGRHLAEKNNTELKLDISAFESYKLRKYELGNLNIIEKFALPEEISRLSTLPTGK 
                  58 
               
               
                 bacterium 
                   
                   
                 transferase 
                   
                   
                 IERFIRKTLRKPVKKPESYIKENITGGFNPKILDLQNNIYLEGYWQSEKYFIEIEDIIRKEFSFKFPATGKNK 
                   
               
               
                   
                   
                   
                 family protein 
                   
                   
                 EILENILNINSVSLHIRRGDYVTNPEVNQVHGVCSLDYYKSCVDFIEKKLESPYFYIFSDDIEWVKNNLQIQS 
                   
               
               
                   
                   
                   
                 [uncultured 
                   
                   
                 QVYYVDHNTVDNAIEDMRLMFSCKHNILANSSFSWWGAWLNSNPDKMVITPRKWFNTTYDSNDLIPERWIKL 
                   
               
               
                   
                   
                   
                 bacterium] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_005822375.1 
                 492366053 
                 protein 
                 27.46 
                   
                 MKIGIIYIVTGPYIKFWNEFYSSSQLYFCVEAEKNYEVFTDSSELASQRLPNVHMHLIEDKGWIVNVSSKSKFI 
                  59 
               
               
                   fragilis ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 CEIRNQLTSYDYIFYLNGNFKFISPIYCDEILPQAEHNYLTALSFSHYLTIHPDHYPYDRNKNCNAFIPYGQGK 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   fragilis ] 
                   
                   
                 YYFQGGFYGGRTQEVLSLSEWCRDAIEADFNKKVIARFHDESYINRYLLTQHPKVLNDKYAFQDIWPYEGEYKA 
                   
               
               
                   fragilis   
                   
                   
                   
                   
                   
                 IVLNKEEVPEDNNLQEMKQNYIDPSLSFLLNDELKFIPISIVQLYGGLGNQMFGYAFYLYIRHISTQERKLLID 
                   
               
               
                 HMW 616 
                   
                   
                   
                   
                   
                 PAPCKRYGNHNGYELPSIFSKICQDIHISDETKNNIRKLRKGTSLSIEEVRASMPQSFKEKKQPIIFYSGCWQC 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VTYVETVKDEIKKDFIFDESKLNEPSAQMLRIIRRSNSVSVHIRRNDYLIGNNEFLYGGICFKSYYEKAISQMY 
                   
               
               
                   
                   
                   
                   
                   
                   
                 TLLKDEPIFIYFTDDPEWVRSNFALDKSYLVDWNKNKDNWQDMYLMSACRHHIIANSSFSWWAAWLGGF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PEKKVIAPSTWLNGMQTPDILPTEWIKIPITPDKKILDRICNHLILHSSYMKQLGLNSGKMGVVIFFFHYARYT 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QNPLYENYAGDLFDELYEEIHKGISFSFLDGLCGIAWAVEYLVHEQFIEGNTDDSLAEIDFKVMQIDPRRFTD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 YSFETGLEGIACYVLSRLLSPRVCSSSLTLDSVYLKDLTEACRKVPVDKANYTRLFLNYIESKEVGYSFKDVLM 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QVLNHSEKAFGSDGLTWQTGLTMIMR 
                   
               
               
                   
               
               
                   Butyrivibrio   
                 WP_022778576.1 
                 551034739 
                 protein 
                 27.46 
                   
                 MIIIQLKGGLGNQMFQYALYKELRSRGKEVKIDDVTGFVDDELRTPVLQRFGIEYDRATREEVVKLTDSKMD 
                  60 
               
               
                 sp. AE3009 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 IFSRIRRKLTGRKTCRIDEESGTFNPDILELDEAYLVGYWQSDKYFRNEDVIAQLRQEFQKRPQEIMTDSASW 
                   
               
               
                   
                   
                   
                 sp. AE3009] 
                   
                   
                 ATLQQIECCQSVSLHIRRTDYIDEEHNHIHNLCFEKYYKGAIDRIRSQYPSAVFFIFTDDKEWCRNHFRGPNF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 FVVELAEKENTDIAEMLLMSSCKHHICANSSFSWWSAWLNDSPEKMVIVPNKWINNRDMDDIYTDRMT 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KMAI 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_004317929.1 
                 490447027 
                 protein 
                 27.43 
                   
                 MKQTIILSGGLGNQMFQYAFFLSMKAKGKSCSLDTTLFQTNKMHNGFELKSVFDIPDSPNQASALHSLLIK 
                  61 
               
               
                   ovatus ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 MLRRYKPKSILTIDEPYTFCPDALESKKSFLMGDWLSPKYFESIKDVVVNAYRFHNIGNKNVDTANEMHGN 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   ovatus ] 
                   
                   
                 NSVSIHIRRGDYLKLPYYCVCNENYYRQAIEQIKDRVDNPIFYVFSNEPSWCDSFMKEFRVNFKIVNWNQG 
                   
               
               
                 
                   ovatus 
                 
                   
                   
                   
                   
                   
                 KDSYQDMYLMTQCKHNIIANSTFSWWGAWLNNNTDKIIVAPSKWFKNSEHNINCKEWLLIDTSK 
                   
               
               
                 CL02T12C04 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Desulfospira 
                 
                 WP_022664368.1 
                 550911345 
                 protein 
                 27.42 
                   
                 MGKKYVETVVNGGLGNQIFQFSAGFALSKRLNLDLVLNISTFDSCQKRNFELYTFPKIKNSFACIKDDDPGVF 
                  62 
               
               
                 
                   joergensenii 
                 
                   
                   
                 [ Desulfospira   
                   
                   
                 SRLRIPFLNFKEKIKQFHESHFFFDPAFFDIREPVRIEGYFQSYKYFEKYSDQLKDILLDIPLTSRLKTVLKV 
                   
               
               
                   
                   
                   
                   joergensenii ] 
                   
                   
                 ISSKKESVSVHIRRGDYISDQGINEVHGTLNEAYYLNSIKLMEKMFPESFFFLFTDDPHYVEENFKFLEDTSC 
                   
               
               
                   
                   
                   
                   
                   
                   
                 IISDNDCLPYEDMYLMANCHHNIIANSSFSWWGAWLNQNPEKIVIAPRKWFSRKILMEKPVMDLLPDDWILL 
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 EOS74299.1 
                 507817890 
                 protein 
                 27.39 
                   
                 MNIIRMTGGLGNQMFQYALFLRLKAQGKEVKFDDRTEYKGEEARPILLWAFGIDYPAAGEEEVNELTDGV 
                  63 
               
               
                   bacterium  10-1 
                   
                   
                 C819_03052 
                   
                   
                 MKFSHRLRRKLFGRKSKEYREKSCNFDQQILEKEPAYFTGYFQSERYFEEVKEQVRKAFQFSGKIWGSVSKEL 
                   
               
               
                   
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 EERIREYQTKIENKSQMPVSVHIRRGDYLENDEAYGGICFDAYYRKAIEMMEEKFPNTVFYIFSNDTGWAK 
                   
               
               
                   
                   
                   
                   bacterium  10-1] 
                   
                   
                 QWIDHFYKEKSRFIVIEGTTEDTGYLDLFLMSKCRAHIIANSSFSWWGAWLDPDQEKIVIAPSKWVNNQD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 MKDIYTREMIKISPKGEVR 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_007832461.1 
                 495107639 
                 protein 
                 27.33 
                   
                 MVVVYVGAGLANRMFQYAFALSLREKGLDVFIDEDSFIPRFDFERTKLDSVFVNVNIQRCDKNSFPLVLRED 
                  64 
               
               
                   dorei ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 RFYKLLKRISEYMSDNRYIERWNLDYLPYIHKKASTNCIFIGFWISYKYFQSSEDAVRKAFTFKPLDSIRNVE 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   dorei ] 
                   
                   
                 LATKLVTENSVAVHFRKNIDYLKNLPNTCPPSYYYEAINYIKKYVPNPKFYFFSDNWDWVRENIRGVEFTAVD 
                   
               
               
                   dorei   
                   
                   
                   
                   
                   
                 WNPSSGIHSHCDMQLMSLCKHNIIANSTYSWWSAYLNENNNKIVVCPKDWYGGMVKKLDTIIPESWIIING 
                   
               
               
                 DSM 17855 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 Firmicutes  
                 WP_021916201.1 
                 547127421 
                 protein 
                 27.33 
                   
                 MVIVKMSGGLGNQMFQYALYRKIQQTGKDVKLDLFSFQDKNAFRRFSLDIFPIEYQTANLEECRKLGECSY 
                  65 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Firmicutes   
                   
                   
                 RPVDKIRRKMFGLKESYYQEDLDKGYQPEILEMNPVYLDGYWQCERYFQDIREKILEDYTFPKKISIESSRLQE 
                   
               
               
                 CAG: 24 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 RIKNTESVSIHIRRGDYLDAANYKIYGNICTIEYYQSAISRMRKLCEKPNFYLFSNDPEWAKEIFGDTEDITIV 
                   
               
               
                   
                   
                   
                 CAG: 24] 
                   
                   
                 EEDKERPDYEDMFLMSRCKHNIIANSSFSWWAAWLNQNENKRVIAPVKWFNNHSVTDVICDDWIRIDGDH 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KGA 
                   
               
               
                   
               
               
                 
                   Clostridium 
                 
                 WP_022031822.1 
                 547299420 
                 epsH 
                 27.3 
                   
                 MIYVNIRGRLGNQLFIYAFARALQKSTNQQITLNYTSFRKHYNNTAMDLEQFNIPEDIMFENSKELPWFAN 
                  66 
               
               
                   hathewayi   
                   
                   
                 [ Clostridium   
                   
                   
                 TDGKVIRILRHYFPKLIRSILQKMNVLMWLGDEYVEVKVNKRRDIYIDGFWQSSRYFKSVYKELKNELIPKME 
                   
               
               
                 CAG: 224 
                   
                   
                 
                   hathewayi 
                 
                   
                   
                 MSKEIKTMGDLINQKESVCVSVRRGDYVTVKKNRDVYYICDEKYLNTSIMRMVELVPNVTWFIFSDDADW 
                   
               
               
                   
                   
                   
                 CAG: 224] 
                   
                   
                 VKDNIVFPGEVFYQPPRVTPLETLYLMKACKHFIISNSSFSWWGQYLSNNDNKIVIGPAKWYVDGRKTDIIE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 EEWIKIEV 
                   
               
               
                   
               
               
                 
                   Syntrophus 
                 
                 YP_462663.1 
                 85860461 
                 alpha-1,2- 
                 27.3 
                   
                 MVIVRLTGGIGNQMFQYAAARRVSLVNNAPLFLDLGWFQETGSWTPRKYELDAFRIAGESASVGDIKDFK 
                  67 
               
               
                 
                   aciditrophicus 
                 
                   
                   
                 fucosyl- 
                   
                   
                 SRRQNAFFRRLPLFLKKRIFHTRQTHIIEKSYNFDPEILNLQGNVYLDGYWQSEKYFSDVDSEIRREFSFQTDP 
                   
               
               
                 SB;  Syntrophus   
                   
                   
                 transferase 
                   
                   
                 AERNRKILERIASCESVSIHIRRGDYVTLPDANAFHGLCFPAYYRLAVEQISRKVVEPVFFVFSDDIAWARGNL 
                   
               
               
                 
                   aciditrophicus 
                 
                   
                   
                 [ Syntrophus   
                   
                   
                 KLGFETCFMDQNGPDRGDEDLRLMIACRHHIIANSSFSWWGAWLCSNPEKIVYAPRKWFNNGLDTPDNI 
                   
               
               
                   
                   
                   
                 
                   aciditrophicus 
                 
                   
                   
                 PASWIRI 
                   
               
               
                   
                   
                   
                 SB] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_005678148.1 
                 491931393 
                 protein 
                 27.27 
                   
                 MKIVKIIGGLGNQMFQYALYLSLKKKYPKEKIKIDISMFETYGLHNGFELKRIFDIDAEYASREEIRELSFYIK 
                  68 
               
               
                   caccae ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 IYKLQRIFRKIFPVRKTECVEKYDFKFMSEVWSNCDRYYEGYWQNWEYFIEAQTEVRSTFTFKKELVGRNAKVI 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   caccae ] 
                   
                   
                 REIQYAKMPVSLHIRRGDYLHHKLFGGLCDLNYYKKAIDYVLNNYDTPQFYLFSNDIEWCKTYILPLVQGYPFI 
                   
               
               
                   caccae   
                   
                   
                   
                   
                   
                 LVDWNSGVESYIDMQLMSCCRINIIANSSFSWWAAWLNDSSEKIVIAPKLWAHSPYGKEIQLKSWLLF 
                   
               
               
                 ATCC 43185 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 WP_022756304.1 
                 551011888 
                 protein 
                 27.24 
                   
                 MIIIEMSGGLGNQMFQYALYKSMLHKGLDVTIDKSIYRDVDHKEQVDLDRFPNVSYIEADRKLSSTLRGYGY 
                  69 
               
               
                 
                   fibrisolvens 
                 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 NDSIIDKIRNKLNKSKRNLYHEDLDKGYQPEIFEFDNVYLNGYWQCERYFKDIKNEIKKDFIFPCFQSGDDKIK 
                   
               
               
                   
                   
                   
                   fibrisolvens ] 
                   
                   
                 ALTIEMESCNSVSLHVRRGDYLKPGLIEIYGNICTEEYYKKSIEYIKERVDNPVFYIFSNDMAWVRDNFKSDDF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 RYVNEDGAFDGMTDMYLMTRCRHNIVANSSFSWWGAWLNKHDDNIVICPNRWVNTHTVTDIICEDWI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 RIDV 
                   
               
               
                   
               
               
                 
                   Parabacteroides 
                 
                 WP_005857874.1 
                 492476819 
                 protein 
                 27.24 
                   
                 MIVGGNDYCKVKVVNIIGGLGNQMFQYAFALSLKEHFPKEEIRIDISHFNYLFVNKVGAANLHNGYELDKIF 
                  70 
               
               
                   distasonis ;  
                   
                   
                 [ Parabacteroides   
                   
                   
                 FNIELKKANAWQLMKLTWFIPNYLISRIARKILPVRNSEYIQNSSDCFFYDPMVYNKQGSCYYEGYWQAIGY 
                   
               
               
                   Parabacteroides   
                   
                   
                 d istasonis ] 
                   
                   
                 YESMRDKLCKIFQHPSPEGKNKQYIENMESSNSVGIHIRRGDYLLSDNFRGICEVDYYKRAIDKILQDGEKHV 
                   
               
               
                 
                   distasonis 
                 
                   
                   
                   
                   
                   
                 FYLFSNDQKWCEEYILPLLGNYEIIFVTGNIGRDSCWDMFLMTHCKDLIIANSSFSWWGAFLNKRGGRVVT 
                   
               
               
                 CL03T12C09 
                   
                   
                   
                   
                   
                 PKRWMNRNIRYDLWMPEWIRI 
                   
               
               
                   
               
               
                 
                   Geobacter 
                 
                 YP_001230447.1 
                 148263741 
                 glycosyl 
                 27.21 
                   
                 MIIARLQGGLGNQMFQYAVGLHLALTHNVELKIDITMFSDYKWHTYSLRPFNIRESIATEEEIKALTDVKMD 
                  71 
               
               
                   uraniireducens ;  
                   
                   
                 transferase 
                   
                   
                 RPYKKIDNFLCRLLRKSQKISATHVKEKHFHYDPDILKLPDNVYLDGYWQSEKYFKEIENIIRQTFIIKNPQL 
                   
               
               
                   Geobacter   
                   
                   
                 family protein 
                   
                   
                 GRDKELACKILSTESVCLHIRRGNYVTDKTTNSVLGPCDLSYYSNCIKSLAGNNKDPHFFVFSNDHEWVSKNL 
                   
               
               
                 
                   uraniireducens 
                 
                   
                   
                 [ Geobacter   
                   
                   
                 KLDYPTIYVDHNNEDKDYEDLRLMSQCKHHIIANSTFSWWSAWLCSNPDKVIYAPQKWFRVDEYNTKDLLPS 
                   
               
               
                 Rf4 
                   
                   
                 
                   uraniireducens 
                 
                   
                   
                 NWLIL 
                   
               
               
                   
                   
                   
                 Rf4] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_016280341.1 
                 511026085 
                 protein 
                 27.21 
                   
                 MIIVKIYEGLGNQLFQYAFARSIQVNGKKVFLDTSGYTDQLFPLCRTSTRRRYQLNCFNIRIKEVEKKNIEKY 
                  72 
               
               
                   bacterium  A4 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 SFLIQEDMFGKLISKLAKLHLWMYKVTIQQNAQEYKESYLNTRGNVYYKGWFQNPKYFSSIRRLLLKEITPKY 
                   
               
               
                   
                   
                   
                   bacterium  A4] 
                   
                   
                 KIRIPAELRELLQEDNIVAVHCRRGDYQYIRNCLPVNYYKKAMAYMEKKLGVPRYLFFSDDLSWVKRQFGNKD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 NNYYIEDYGKFEDYQELMIMSRCRNFIIANSTFSWWAAWLCSYENKVVIMPRVWTYVGGQGVEMSDFPA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DWIRI 
                   
               
               
                   
               
               
                 
                   Colwellia 
                 
                 YP_270849.1 
                 71282201 
                 alpha-1,2- 
                 27.15 
                   
                 MKVVRVCGGFGNQLFQYAFYLAVKHKFNETTKLDIHDMASYELHNGYELERIFNLNENYCSAEEKLAVQST 
                  73 
               
               
                   psychrerythraea ; 
                   
                   
                 fucosyl- 
                   
                   
                 KNIFTKLLKEIKKYTPFIPRTYIKEKKHLHFSYQEVDLGTKDTSIYYRGSWQNPQYFNSIASEIREKLTFPEF 
                   
               
               
                 
                   Colwellia 
                 
                   
                   
                 transferase 
                   
                   
                 TEPKSLALHQEISEHETVAVHIRRGDYLKHKALGGICDLPYYQNAIKEIEGLVEKPLFVIFSDDITWCRANIN 
                   
               
               
                   psychrerythraea   
                   
                   
                 [ Colwellia   
                   
                   
                 VEKVRFVDWNSGEQSFQDMHLMSLCFHNIIANSSFSWWGAWLNANPNKIVISPNKWIHYTDSMGIVPSEWIKV 
                   
               
               
                 34H 
                   
                   
                 
                   psychrerythraea 
                 
                   
                   
                 ETSI 
                   
               
               
                   
                   
                   
                 34H] 
                   
                   
                   
                   
               
               
                   
               
               
                   Roseobacter  sp. 
                 WP_009810150.1 
                 497495952 
                 alpha-1,2- 
                 26.96 
                   
                 MITSRLHGRLGNQMFQYAAARALAHRLGCGVALDGRGAELRGEGVLTRVFDLPLSAAPKLPPLKQHAPLR 
                  74 
               
               
                 MED193 
                   
                   
                 fucosyl- 
                   
                   
                 YGLWRGLGLAPRFRRERGLGYNTAFETWEDGCYLHGYWQSERYFEEISDLIRADFTFPDFSNRQNAEMAA 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 RIMEDNAISLHVRRGDYVALSAHVLCDQAYYEAALTRLLEGLSQDAPTVYVFSDDPDWAKANLPLPCKKVV 
                   
               
               
                   
                   
                   
                 [ Roseobacter   
                   
                   
                 VDFNGPETDFEDMRLMSLCKHNIIGNSSFSWWAAWLNANPQKRVAGPANWFGDPKLSNPDILPSQWLK 
                   
               
               
                   
                   
                   
                 sp. MED193] 
                   
                   
                 VAP 
                   
               
               
                   
               
               
                 
                   Cesiribacter 
                 
                 WP_009197396.1 
                 496488826 
                 Glycosyl 
                 26.89 
                   
                 MMIVRLCGGLGNQLFQYAVGKQLSVKNNIPLKIDDSWLRLPDARKYRLQFFQIEEPLASPQEVERFVGPYES 
                  75 
               
               
                   andamanensis ;  
                   
                   
                 transferase 
                   
                   
                 QSLYARLYRKVQNMLPRHRRRYFQESGFWAYEPELMRIRSQVFLEGFWQHHAYFTRLHPQVLEALQLREE 
                   
               
               
                   Cesiribacter   
                   
                   
                 family 11 
                   
                   
                 YRQEPYAVLDQIREDAASVSLHIRRGDYVSDPYNLQFFGVMPLSYYQQAVAYMQEQLHAPTFYIFSDDLD 
                   
               
               
                 
                   andamanensis 
                 
                   
                   
                 [ Cesiribacter   
                   
                   
                 WARAHLKLQAPMVFVDIEGGRKEYLELEAMRLCRHNILANSSFSWWGAYLNTNPHKRVIAPRQWVADPE 
                   
               
               
                 AMV16 
                   
                   
                   andamanensis ] 
                   
                   
                 LKDKVQIQMPDWILL 
                   
               
               
                   
               
               
                 
                   Rhodopirellula 
                 
                 WP_008679055.1 
                 495954476 
                 glycosyl 
                 26.89 
                   
                 MIATRLIGGLGNQMFQYAYGFSLARRRSERLVLDVSAFESYDLHALAIDQFDISAARMTQAEFARIPGRYRG 
                  76 
               
               
                   sallentina ;  
                   
                   
                 transferase 
                   
                   
                 KSRWAERVANFAGGLQSCDKRPLRLRREKPFGFAEKYLAEGSDLYLDGYWQSERYFPGLQAELKKEFQLKR 
                   
               
               
                   Rhodopirellula   
                   
                   
                 family 11 
                   
                   
                 GLSDESSRVLDEIQSSMSVAMHVRRGDYVTNAETLRIYRRLDAEYYRKCLNDLRQRFSNLNVFVFSNDIQW 
                   
               
               
                   sallentina   
                   
                   
                 [ Rhodopirellula   
                   
                   
                 CQDHLDVGLKQRPVTHNDATTAIEDMFLMSQCDHSIIANSSFSWWAAFLGRSDAQRRVYYPDPWFNPG 
                   
               
               
                 SM41 
                   
                   
                   sallentina ] 
                   
                   
                 TLNGDSLGCANWVSESSISVSRPSRAA 
                   
               
               
                   
               
               
                   Butyrivibrio   
                 WP_022762282.1 
                 551018054 
                 protein 
                 26.85 
                   
                 MIIIRMMGGLGNQMFQYALYLQLKALGKEVKIDDVYGFRDDPQRDPVLEKMYGITYTKASDAEVVDITDS 
                  77 
               
               
                 sp. AD3002 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 HLDIFSRIRRKLFGRKSHEYIEETGLFDPKVFEFETAYLNGYFQSDKYFPDKEVLAQLRREFVIKPDDVFTSA 
                   
               
               
                   
                   
                   
                 sp. AD3002] 
                   
                   
                 DSWELYRQIRETESVSIHVRRGDYLLPGTVETFGGICDNDYYKRAIDRMVSEHPDAIFFVFTSDKEWCEQNVS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 GKKFRIVDTKEENDDAADLLLMSLCKHHILANSSYSWWSAWMNDSPEKTVIVPSKWLNTKPMDDIYTSRM 
                   
               
               
                   
                   
                   
                   
                   
                   
                 TKI 
                   
               
               
                   
               
               
                 
                   Segetibacter 
                 
                 WP_018611017.1 
                 517440157 
                 protein 
                 26.78 
                   
                 MVVVKLIGGMGNQMFQYAIGRHLAIKNKCPLYFDHIELENKNTANTPRNYELDIFNVQYQKNPFLQSNRF 
                  78 
               
               
                 
                   koreensis 
                 
                   
                   
                 [ Segetibacter   
                   
                   
                 VAKVYHKLFSVQRIKEPDFTFHPHILNVQGNIHLNGYWQNENYFKEIEEIIRQDFTFKTPANEKIESILQQIA 
                   
               
               
                   
                   
                   
                   koreensis ] 
                   
                   
                 ATNSVSLHVRRGDYITLTEANQFHGVCSDTYYQKAIAKIKEAIPAPHLFVFSDDIHWVKQNMPFTEEHTFVDG 
                   
               
               
                   
                   
                   
                   
                   
                   
                 NTGKNSFEDLRLMAACRHNILANSSFSWWAGWLNKNPEKMVIAPEKWFRAVHTDIVPPSWIKM 
                   
               
               
                   
               
               
                   Amphritea   
                 WP_019621022.1 
                 518450815 
                 protein 
                 26.76 
                   
                 MVIVRLIGGLGNQLFQYAYALSLLEQGYDVKLDASAFESYTLHGGFGLGEYAERLEVATTEEVDMVSRVGRI 
                  79 
               
               
                 
                   japonica 
                 
                   
                   
                 [ Amphritea   
                   
                   
                 STLLRKLQGKKSRRVIKESNFSYDEKMLTPEDSHYLVGYFQSELYFNKIRGELLSALDLKHKLSPYTEASYLA 
                   
               
               
                   
                   
                   
                   japonica ] 
                   
                   
                 IADASVSVSMHIRRGDYVSDKAAHNTHGVCSLDYYYAAVTFFEERYPDVDFYIFSDDIEWVKENLNVQRAHYI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SSEEKRFAGEDIYLMSQCDHNIVANSSFSWWGAWLNANEDKIVVAPRQWYADSNMQRLSKTLVPDTWI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 RL 
                   
               
               
                   
               
               
                 
                   Desulfovibrio 
                 
                 YP_002437106.1 
                 218887785 
                 glycosyl 
                 26.76 
                   
                 MRPWVDIFGGLGNQMFQYAAAKSLAERLGVRLELDVSMFSGDPLRAFSLGEFAITDHVRGKSRSSLLVRF 
                  80 
               
               
                   vulgaris ;  
                   
                   
                 transferase 
                   
                   
                 ARSLGFGSSSKCVEPFFHYWEGINEIEAPVHMHGYWQSEKYFKAYEDLIRRTFSFSACEGVASSGKYAGVSS 
                   
               
               
                 
                   Desulfovibrio 
                 
                   
                   
                 family protein 
                   
                   
                 PMSVSVHLRRGDYKEQKNVVVHGILGREYYDAAYSIIKQGCPSACFFVFTDAINEAVDFFSHWNDVLFVDG 
                   
               
               
                   vulgaris  str.  
                   
                   
                 [ Desulfovibrio   
                   
                   
                 NNQYQDMYLMSQCRHHIIANSSYSWWGAWLGAFSDGMTVAPKMWFAYDVLKEKSIKDLFPEDWIVL 
                   
               
               
                 ′Miyazaki F′ 
                   
                   
                   vulgaris  str. 
                   
                   
                   
                   
               
               
                   
                   
                   
                 ′Miyazaki F′] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Spirosoma 
                 
                 WP_020606886.1 
                 522095677 
                 protein 
                 26.76 
                   
                 MIISRITSGLGNQLFQYAVARHLSLKNKTSLYVDLSYYLYQYHDDTSRNFKLGNFSVPYHTLQQSPVEYVSKA 
                  81 
               
               
                 
                   spitsbergense 
                 
                   
                   
                 [ Spirosoma   
                   
                   
                 TKLLPNRSLRPFFLFQKERQFHFDEQILQSRAGCVILEGFWQSEAYFRDNADTIRRDLQLSGTPSPEFNQYRE 
                   
               
               
                   
                   
                   
                   spitsbergense ] 
                   
                   
                 LIRETPMSVSIHVRRSDYVNHPEFSQTFGFVGIDYYKRAIELARKELANPRFFVFSDDKEWSKTNLPLGEDSV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 FVQNTGLNGDVADLVLMSHCQHHIIANSSFSWWGAWLNPNAGKLVITPKNWYKNKPAWNTKDLLPPT 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WLSI 
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_016292012.1 
                 511037988 
                 protein 
                 26.73 
                   
                 MNIIRMSGGIGNQMFQYALYLKLVSLGKEVKFDDVTEYELDNARPIMLSVFGIDYPKASREELVELTDASM 
                  82 
               
               
                   bacterium  28-4 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 DFLSRVRRKIFGRKSGEYHEASADYDETVLEKEHAYLCGCFQSERYFKDIEYEVREAYRFRNVVVPEEIRGGI 
                   
               
               
                   
                   
                   
                   bacterium  28- 
                   
                   
                 ETYERQIGESLSVSIHIRRGDYLDAADVYGGICFDAYYNQAIRYMIKKYENPSFFVFTNDTFWAEKWCEVRER 
                   
               
               
                   
                   
                   
                 4] 
                   
                   
                 ETGKRFTVIKGTDEETGYIDLMLMSRCKAHIIANSSFSWWGAWLDASPDKCVVAPVKWINTRECRDIYTED 
                   
               
               
                   
                   
                   
                   
                   
                   
                 MVRIGSNGKISFSNCSSL 
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_016302211.1 
                 511048325 
                 protein 
                 26.71 
                   
                 MVVVRIWEGLGNQLFQYAYARALSLRTKDRVYLDISEYEMSPKPVRKYELCHFKIKQPVINCGRIFPFVNKD 
                  83 
               
               
                   bacterium  COE1 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 SFYTKNNQYLRYFPAGLIKEEDCYFKRDFCELKGLLYLKGWFQSEKYFKEFESHIREEIYPRNKIKITRGLRKI 
                   
               
               
                   
                   
                   
                 
                   bacterium 
                 
                   
                   
                 LNSDNTVSVHIRRGDFGKDHNILPIEYYENSKRVILERVDNPYFIIFSDDILWVKENMNFGLNCFYMDKEYSYK 
                   
               
               
                   
                   
                   
                 COE1] 
                   
                   
                 DYEELMIMSRCKHNIIANSTFSWWGAWLNPSKDKIVIAPKKWFLYNPKKDFDIVPNDWIRV 
                   
               
               
                   
               
               
                   Parabacteroides ; 
                 WP_005867692.1 
                 492502331 
                 alpha-1,2- 
                 26.69 
                 FutZB 
                 MKIVNIIGGLGNQMFQYAFAVALKAKYPNEEVFIDTQHYKNAFIKVYHGNNFYHNGYEIDKVFPNATLEPA 
                  84 
               
               
                   Parabacteroides   
                   
                   
                 fucosyl- 
                   
                   
                 RPKDLMKVSFYIPNQVLARAVRRIFPKRKTEFVTDQQPYVFIPEALSVIDDCYFDGYWMTPLYFDKYRDRILK 
                   
               
               
                 sp. 20_3;  
                   
                   
                 transferase 
                   
                   
                 EFTFRPFDTKENLELEPLLKQDNSVTVHIRRGDYVGSSSFGGICTLDYYRNAIREAYNLITSPEFFIFSNDQKW 
                   
               
               
                 
                   Parabacteroides 
                 
                   
                   
                 [ Parabacteroides ] 
                   
                   
                 CMENMRNEFGDAKVHFIAHNRGADSYRDMQLLSIARCNILANSSFSWWGAYLNQRKNCFIICPHKWHN 
                   
               
               
                 
                   distasonis 
                 
                   
                   
                   
                   
                   
                 TLEYSDLYLPTWIKI 
                   
               
               
                 CL09T03C24 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_002561428.1 
                 488624717 
                 protein 
                 26.62 
                   
                 MFVIRLIGGVGNQLFQYTFGQFLRHKFGVEVCYDIVAFDTVDKGRNLELQLLDESLPLFETSNFFFSKYKSWK 
                  85 
               
               
                 HPS0048 
                   
                   
                 [ Bacteroides  sp. 
                   
                   
                 KRLFLYGFLLKKNNKYYTKYAPEEISLFTEKGLSYFDGWWQYPALLRDTINNMEDFFIPKQPIPVQIQKYYNEI 
                   
               
               
                   
                   
                   
                 HPS0048] 
                   
                   
                 LLNNFAVALHVRRGDYFTSKYAKTYAVCNVEYYTSAVNLMCEKLRSCKFYVFSDDLDWVKSNLILPSNTVYV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KNYDINSYWYIYLMSLCRHIIISNSSFSWWGATLNRNFHKIVIAPKYWSTKKNNTLCDNSWIKI 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_016267863.1 
                 511013468 
                 protein 
                 26.58 
                   
                 MKIINILGGLGNQMFEYAMYLALKNAHSEEEILCSTRSFCGYGLHNGYELGRIFGIQVKEASLLQLTKLAYPFF 
                  86 
               
               
                   thetaiotaomicron ; 
                   
                   
                 [ Bacteroides   
                   
                   
                 NYKSWQVMRHWLPVRKTMTRGAINIPFDYSQVMREDSVYYDGYWQNEKNFLHIREEILTAYTFPKFDDE 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   thetaiotaomicron ] 
                   
                   
                 KNQELADIIVKSNAVSCHIRRGDYLKEINMCVCTSSYYAHAISYMNEEINPNLYCVFSDDIEWCRNNICELM 
                   
               
               
                 
                   thetaiotaomicron 
                 
                   
                   
                   
                   
                   
                 GEDKKIIFIDWNKGEKSFRDMQLMSLCKHNIIANSSFSWWGAWLNRNDKKIVVAPTRWIASEVKNDPLCD 
                   
               
               
                 dnLKV9 
                   
                   
                   
                   
                   
                 SWKRIE 
                   
               
               
                   
               
               
                 
                   Desulfovibrio 
                 
                 YP_389367.1 
                 78357918 
                 glycosyl 
                 26.56 
                   
                 MKFVGVWILGGLGNQMFQFAAAYALAKRMGGELRLDLSGFKKYPLRSYSLDLFTVDTPLWHGLPMSQRR 
                  87 
               
               
                   alaskensis ; 
                   
                   
                 transferase 
                   
                   
                 FRIPMDAWTRGSRLPLVPSPPFVMAKEKNFAFSPIVYELQQSCYLYGYWQSYRYFQDVEDDIRTLFSLSRFA 
                   
               
               
                 
                   Desulfovibrio 
                 
                   
                   
                 [ Desulfovibrio   
                   
                   
                 TLELAPVVAQLNEVESVAVHLRRGDYITDAASNAVHGVCGIDYYQRSMSLVRRSTTKPIFYIFSDEPEVAKKL 
                   
               
               
                   alaskensis  G20 
                   
                   
                   alaskensis  G20] 
                   
                   
                 FATEDDVVVMPSRRQEEDLLLMSRCKHHIIANSSFSWWAAWLGKRASGLCIAPRYWFARPKLESTYLFDLI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PDEWLLL 
                   
               
               
                   
               
               
                   Prevotella   
                 ETD21592.1 
                 564721540 
                 protein 
                 26.56 
                   
                 MDIVVIFNGLGNQMSQYAFYLAKRKSGSRCHCIFHNVSTGFHNGSELDKVFGIKYEKGIFSKLLSKIYDIFDGI 
                  88 
               
               
                 
                   oralis 
                 
                   
                   
                 HMPREF1199_00667 
                   
                   
                 PKLRKKLNSLGIHIIREPRNYDYTASLLPRVSRWGLNYFVGGWHSEKYYTEILQEIKNTFSFKIDDEIKDIDFY 
                   
               
               
                 CC98A 
                   
                   
                 [ Prevotella   
                   
                   
                 EFYSLIHNDINSVSLHIRRGDYVGANEYSYFQFGGVATLEYYHKAIDEIYQRIENPTFYVFSDDIGWCKTTFLK 
                   
               
               
                   
                   
                   
                   oralis  CC98A] 
                   
                   
                 NNFIFVDCNCGEKSWRDMFLISQCKHHIIANSTFSWWGAWLSIFHNSITICPKEFIKGVVIRDVYPDTWIKLSS 
                   
               
               
                   
               
               
                 
                   Comamonadaceae 
                 
                 YP_008680725.1 
                 550990115 
                 glycosyl- 
                 26.54 
                   
                 MASKISKIIPRIFGGLGNQLFIYAAARRLALVNGAELALDDVSGFVRDHEYNRHYQLDHFNIPCRKATAAERL 
                  89 
               
               
                   bacterium  CR 
                   
                   
                 transferase 
                   
                   
                 EPFARVRRYLKRKWNQRLPFEQRKYLVQESVDFDERLLTFKPRGTVYLEGYWQSEDYFKDIEPQIRADLRIH 
                   
               
               
                   
                   
                   
                 [ Comamonadaceae   
                   
                   
                 PPTDTVNQQMAERIRATNAVAVHVRFFDAPAQSALGVGGNNAPGDYYQRAIKVMQEQAPDAQYYIFSD 
                   
               
               
                   
                   
                   
                 
                   bacterium 
                 
                   
                   
                 QPQAARARIPLRDDHVTLVNHNQCDAVAYADLWLISQCQHFIIANSTFSWWGAWLGKTPESIVIAPGFEK 
                   
               
               
                   
                   
                   
                 CR] 
                   
                   
                 REGAMFWGFRGLLPDRWVKL 
                   
               
               
                   
               
               
                 
                   Vibrio 
                 
                 WP_022596860.1 
                 550250577 
                 WblA protein 
                 26.51 
                   
                 MKDSRIVKLNGGLGNQMFQFALAFALKKKLNVAVKFDTELLDTNRTEFKLSLERFGLIVDKLTITEKFKYKGL 
                  90 
               
               
                   nigripulchritudo ;  
                   
                   
                 [ Vibrio   
                   
                   
                 ESCKYRKICNWISNFTTINIHKGYYKEKERGVYDRGIFDSNVKYIDGYWQNQEYFNDFRSELLNKFNLNGKV 
                   
               
               
                   Vibrio   
                   
                   
                   nigripulchritudo ] 
                   
                   
                 SNHAIQYLKEITSVQNSVSIHVRRGDYLLLDVYRNLTLDYYSEAIKLVRITNPDSKFFIFSNDINWCKSNFKS 
                   
               
               
                 nigripulchritudo 
                   
                   
                   
                   
                   
                 VDNAIFVDSTVDEFDDMFLMSKCKTNIIANSTFSWWAAWLNNNSGKIVYCPKKWRNDTTEVHKGLPEGWNI 
                   
               
               
                 AM115; 
                   
                   
                   
                   
                   
                 IDK 
                   
               
               
                 
                   Vibrio 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   nigripulchritudo 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 FTn2;  Vibrio   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   nigripulchritudo 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Pon4;  Vibrio   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   nigripulchritudo 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 SO65 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Sulfurospirillum 
                 
                 YP_003304837.1 
                 268680406 
                 glycosyl 
                 26.48 
                   
                 MIIIKIMGGLTSQMHKYALGRVLSLKYNVPLKLDLTWFDNPKSDTPWEYQLDYFNINATIATVSEIKKLKGN 
                  91 
               
               
                   deleyianum ;  
                   
                   
                 transferase 
                   
                   
                 NLFNRIARKIEKFFSIRIYKKSYINKSFISISDFHKLKSDIYLDGEWNGFKYFEDYQDTIKNELTLKRGSSIN 
                   
               
               
                 
                   Sulfurospirillum 
                 
                   
                   
                 family protein 
                   
                   
                 IQNTIKELKSSDNSVFLHIRRGDYLSNKNAAAFHAKCSLDYYYKAIQIVKEKIDNPIFYIFSDDILWVKKNFV 
                   
               
               
                 
                   deleyianum 
                 
                   
                   
                 [ Sulfurospirillum   
                   
                   
                 INESCRFMEKNQNFEDLLLMSYCKHGITANSGFSLMAGWLNQNKDKMIIVPQTWVNDDRININILNSLEQDNF 
                   
               
               
                 DSM 6946 
                   
                   
                 
                   deleyianum 
                 
                   
                   
                 TIIR 
                   
               
               
                   
                   
                   
                 DSM 6946] 
                   
                   
                   
                   
               
               
                   
               
               
                   Escherichia   coli ; 
                 WP_001581194.1 
                 486318742 
                 glycosyl 
                 26.47 
                   
                 MTFIVRLTGGLGNQMFQYALARSLAKKYNARLKLDISYYHNQPHKDTPRTFELNQLCIVDNILNSSSFSEKFL 
                  92 
               
               
                   Escherichia   coli   
                   
                   
                 transferase 11 
                   
                   
                 YIYDKLRVKLSKKISLPYFRNIVTPVNFNCIDFAEDKDYYFLGHFQELSNIYSIDESLRSEFKPNQEIMNLAHQ 
                   
               
               
                 Jurua 18/ll; 
                   
                   
                 family protein 
                   
                   
                 SKIYELIKQSRGSVALHIRRGDYVTNKNAAEHHGVIGLSYYVNALSYLENVSEFFDVFVFSDDPEWARKNIKNS 
                   
               
               
                   Escherichia   coli   
                   
                   
                 [ Escherichia   
                   
                   
                 RNLFFCDEGNCRYSKKYSTIDMYLMSQCDHFIIANSTYSWWAAWLGNYPSKHVVAPARWNANNSPYPIL 
                   
               
               
                 180600;  
                   
                   
                   coli ] 
                   
                   
                 QNWKAIHE 
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.1;  
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.2; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.3; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.4; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.7; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.9; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.10; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.11; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.12; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.13; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.14; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Escherichia   coli   
                   
                   
                   
                   
                   
                   
                   
               
               
                 P0304777.15 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Firmicutes   
                 WP_021914998.1 
                 547109632 
                 protein 
                 26.44 
                   
                 MVGVQLSGGLGNQMFEYALYLKLKSMGKDVRIDDVTCYGAQEKQRVNQLSVFGVSYEHMTKQEYEQITD 
                  93 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Firmicutes   
                   
                   
                 SSMSPLHRARRLLCGRKDLSYREASCNYDPEILRREPALLLGYFQTERYFADIKDQVREAFTFRNLTLTKESAA 
                   
               
               
                 CAG: 24 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 MEQQMKECESVSVHIRRGDYLTPANQALFGGICDLDYYHRAVAEIRKRKPDVKFFLFSNDMEWTKEHFCG 
                   
               
               
                   
                   
                   
                 CAG: 24] 
                   
                   
                 SEFVPVEGNSEQAGEQDLYLMSCCKNHILANSSFSWWGAWLDNGKDKLVIAPEKWMNGRGCCDIYTDE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 MIRV 
                   
               
               
                   
               
               
                   Amphritea   
                 WP_019622926.1 
                 518452719 
                 protein 
                 26.42 
                   
                 MVKIKIIGGLGNQMFQYAAAKSLAVLNNTRVSANVSVFSNYKTHPLRLNKLNCDCEFDFTRDFRLVLSGFPL 
                  94 
               
               
                 
                   japonica 
                 
                   
                   
                 [ Amphritea   
                   
                   
                 LGSAFSKKSMLLNHYVEKDLLFDSSFFDLDDNVLLSGYFQSEKYFSNIRELLIQEFSLDDRLTEAELAINNKIE 
                   
               
               
                   
                   
                   
                   japonica ] 
                   
                   
                 SCNSIAIHIRRGDYITDLSANNIHGICSEEYFEKALNYLDSINVLSDPTTTLFIFSDDILWCKDNLAFKYRTVF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VEGSVDRPEVDIHLMSKCKHQVISNSTFSWWGAWLNTNLDKCVIAPLKWFNSLHDSTDIVPKQWMRL 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_005923045.1 
                 492689153 
                 protein 
                 26.41 
                   
                 MKQTIIMSGGLGNQMFQYALYCSMREKGIRVKIDISLYEFNRMHNGYMLDYAFGLNISHNKINKYSVLWT 
                  95 
               
               
                   salyersiae ; 
                   
                   
                 [ Bacteroides   
                   
                   
                 RLIRSNRAPFLLFREDESRFCDDVFTTYKPYIDGCWIDERYFFNIKKKIISQFSFHNIDQKNLMVANMMKVCN 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   salyersiae ] 
                   
                   
                 SVSLHIRRGDYLSQSMYNICNESYYKSAIEYIISRVEDSKFFIFSDDPEWCKYFMEKFNVDYEIIQHNFGKDS 
                   
               
               
                 
                   salyersiae 
                 
                   
                   
                   
                   
                   
                 YKDMYLMTQCKHNIIANSTFSWWGAWLNNNAGKNVVCPSVWINGRDFNPCLEEWYHI 
                   
               
               
                 WAL 10018 = 
                   
                   
                   
                   
                   
                   
                   
               
               
                 DSM 18765 = 
                   
                   
                   
                   
                   
                   
                   
               
               
                 JCM 12988 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_005786334.1 
                 492241663 
                 protein 
                 26.38 
                   
                 MDIILLHNGLGNQMSQYAFYLSKKKNGIHTSYICLSNDHNGIELDKVFGVECQMGCKKIFLLFILRLLMSNRT 
                  96 
               
               
                   fragilis ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 GFLIRKVNLLFSKIKIKLITENLDYSFHPSFLSASPYCLAFWVGGWHHPQYYSEISSQIKEAFTFKRSLLDERN 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   fragilis ] 
                   
                   
                 ICIEKRMREPNSVCLHIRRGDYLTGINYELFGKVCNEQYYQKAIDYIEGKLSDICYYVFSNDMEWAKKILLGKN 
                   
               
               
                 
                   fragilis 
                 
                   
                   
                   
                   
                   
                 AVFVDWNRGEESWKDMYLMSKCSNLIIPNSTFSWWAAWLCEHPVNIVCPKLFVYGDEQSDIYLDNWHKIE 
                   
               
               
                 CL03T00C08; 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Bacteroides   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   fragilis 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CL03T12C07 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_007486843.1 
                 494751435 
                 protein 
                 26.37 
                   
                 MMGIEKTNMVIVRLWGGIGNQLFQYSFGEFLREKYQVDVIYDIASFGKSDKLRKLELSVVVPGIPVTTDISFS 
                  97 
               
               
                   nordii ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 KYVGTKNRLLRFIYGLKNSFIEEKYFSDEQLFKYLSKRGDVYLQGYWQKTIYAETLRRKGSFFLSQEEPIVLHT 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   nordii ] 
                   
                   
                 IKAKIQEAEGAIALHVRRGDYFSSKHINTFGVCDAHYYEKAVDIMRGRVSNAMIFVFSDDLDWVRRYVNLPT 
                   
               
               
                   nordii   
                   
                   
                   
                   
                   
                 NVIYVPNYDIPQYWYIYLMSLCRHNIISNSSFSWWGAFLNMNTNKIVVSPSKWTLNSDKTIALDEWFKI 
                   
               
               
                 CL02T12C05 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 YP_003829743.1 
                 302669783 
                 glycosyl 
                 26.37 
                   
                 MECSMIIIKFCGALGNQLFQYALYEKMRILGKDVKADISAFGDGNEKRFFYLDELGIEFNIASADEIAEYLNR 
                  98 
               
               
                   proteoclasticus ; 
                   
                   
                 transferase 11 
                   
                   
                 KTIRFVPGFLQHRHYYFEKKPYVYNKKILSYDDCYLEGYWQNYRYFDDIKDELLKHMKFPCLPLEQKKLAEKM 
                   
               
               
                 
                   Butyrivibrio 
                 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 ENENSVAVHVRMGDYLNLQDLYGGICDADYYDRAFSYIEGNISNPVYYGFSDDVDKASALLAKHKINWIDY 
                   
               
               
                 
                   proteoclasticus 
                 
                   
                   
                 
                   proteoclasticus 
                 
                   
                   
                 NSEKGAIYDLILMSKCKNNIIANSSFSWWGAYLEYNNGKVVVSPNRWMNCFENSNIAYWGWISL 
                   
               
               
                 B316 
                   
                   
                 B316] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 YP_003574648.1 
                 294674032 
                 family 11 
                 26.33 
                   
                 MRIVKVLGGLGNQMFQFALYKALQKQYPEERVLLDLHCFNGYHKHRGFEIDSVFGVTYEKATLKEVASLAY 
                  99 
               
               
                   ruminicola ;  
                   
                   
                 glycosyl 
                   
                   
                 PYPNYQCWRIGSRILPVRKTMLKEEPNFTLEPSALSLPDSTYYDGYWQHEEYFMHIREEILSTYAFPAFDDER 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                 transferase 
                   
                   
                 NKTTAQLAASTNSCSIHIRRGDYLTDPLRKGTTNGNYVIAAIKEMQQEVKPEKWLVFSDDIAWCQQHLAST 
                   
               
               
                   ruminicola  23 
                   
                   
                 [ Prevotella   
                   
                   
                 LDATNTIYIDWNTGANSIHDMHLMALCRHHIIANSSFSWWGAWLSQQDGITIAPSNWMNLKDVCSPVP 
                   
               
               
                   
                   
                   
                   ruminicola  23] 
                   
                   
                 DNWIKI 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_007135533.1 
                 494223898 
                 protein 
                 26.33 
                   
                 MKIIKIIGGLGNQMFQYALAIALQQQYKDEEIRLDLNCFRGYNKHQGYLLDEIFGRRFRAASLQEVARLAWP 
                 100 
               
               
                   salivae ;  
                   
                   
                 [ Prevotella   
                   
                   
                 YPHYQLWRVGSRVLPRRQTMVCEPADGSFSPDVLTLEGNRYYDGYWQDERYFKAYRKEIIEAFKFSPFVGD 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                   salivae ] 
                   
                   
                 GNRHVENMLRNERFASLHVRRGDYLNDALYQNTCGIDYYQRAISQMNAMANPSCYFIFSDDIAWCKTHIE 
                   
               
               
                 
                   salivae 
                 
                   
                   
                   
                   
                   
                 PLCEGHRPYYIDWNKGKEAYRDMQLMALCKYHIIANSSFSWWGAWLNDAEDGITIAPQQWYSHGNKPS 
                   
               
               
                 DSM 15606 
                   
                   
                   
                   
                   
                 PASESWIKV 
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_016299568.1 
                 511045640 
                 protein 
                 26.3 
                   
                 MNIVRISDGLGNQMFQYAYARKISILSRQRTYLDIRFINNEDLVKKGNHVQFRKKLGHRKYGLSHFNVSLQI 
                 101 
               
               
                   bacterium  COE1 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 ADLKMLSHWEYLIQSNCMQQLIYSLSMQDKWIWRYRHEEVNYDGMLSKVELLFPTYYQGYFFALKYYDDI 
                   
               
               
                   
                   
                   
                 
                   bacterium 
                 
                   
                   
                 KHILQHDFSLKDKMKLLPELRDALYNRNTISLHVRRGDFLEINRDISGSEYYEKAVQMIGSKVESPIFLIFSDD 
                   
               
               
                   
                   
                   
                 COE1] 
                   
                   
                 IEWVKEHIRIPNDKIYVSGIGYEDYEELTIMKHCKHNIIANSTFSYWAAYLNSNKDKIVICPKHWRERIIPKDW 
                   
               
               
                   
                   
                   
                   
                   
                   
                 ICI 
                   
               
               
                   
               
               
                   Bacteroides   
                 WP_007842931.1 
                 495118115 
                 alpha-1,2- 
                 26.28 
                   
                 MIVVNVNAGLANQMFHYAFGRGLEAKGWNIYFDQTNFKPRKEWSFENVQLQDAFPNLGLKMMPEGKF 
                 102 
               
               
                   dorei ; 
                   
                   
                 fucosyl- 
                   
                   
                 KWICVNNTNKLSKGLHLAMINLHNLIGDEKYIFETTYGYDPDIEKEITKNCILKGFWQSEKYFAHCKDDIRKQ 
                   
               
               
                   Bacteroides   
                   
                   
                 transferase 
                   
                   
                 FSFLPFDEEKNIVIMNKMVKENSVAIHLRKGADYLKSELMGKGLCGVEYYIKAIEYIKKNIDNPVFYVFTDNP 
                   
               
               
                 
                   dorei 
                 
                   
                   
                 [ Bacteroides   
                   
                   
                 VWVKNNLPKFDYILVDWNEVAGKKNFRDMQLMSCAKHNIIANSTYSWWGAWLNPNPNKIVIGPAKFFN 
                   
               
               
                 5_1_36/D4 
                   
                   
                   dorei ] 
                   
                   
                 PINNFFSSSDIMCEDWVKI 
                   
               
               
                   
               
               
                   Roseobacter  sp. 
                 WP_008210047.1 
                 495485361 
                 alpha-1,2- 
                 26.28 
                   
                 MLSKDPGMITTRLHGRLGNQMFQYAAGRALAARLGVPLALDSRGAKLRGEGVLTRVFDLPLAQPLSLPPLK 
                 103 
               
               
                 SK209-2-6 
                   
                   
                 fucosyl- 
                   
                   
                 QDAPLRYAAWRLTGRTPRFRREQGLGYNPAFETWGDDSYLHGYWQSEAYFDSIADQIRQDFTFPEFSNSQ 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 NREMAQRIAGSTAISLHVRRGDYVALAAHVLCDQAYYEAALTRILEGVEGSPTVYVFSDDPNWAKENLPLP 
                   
               
               
                   
                   
                   
                 [ Roseobacter   
                   
                   
                 CEKVVVDFNGPDTDFEDMRLMSLCQHNIIGNSSFSWWAAWLNTHNEKRVAGPAHWFGNPKLQNPDIL 
                   
               
               
                   
                   
                   
                 sp. SK209-2-6] 
                   
                   
                 PESWLKISV 
                   
               
               
                   
               
               
                 alpha 
                 WP_020056701.1 
                 518900826 
                 protein [alpha 
                 26.26 
                   
                 MIYSRIRGGLGNQLFQYCVARSLADNLGTSLGLDVRDFNENSPYLMGLKHFNIRADFNPPGMIEHKKNGYF 
                 104 
               
               
                 proteobacterium 
                   
                   
                 proteobacterium 
                   
                   
                 RYLIDVVNGKQKFVYKEPHLNFDKNIFSLPNSSYLKGYWQTEKYFIKNKVNILNDLKIISHQSDKNKTISSKIA 
                   
               
               
                 SCGC AAA076-C03 
                   
                   
                 SCGC 
                   
                   
                 NNTSVSLHIRRGDYISNSAYNSTHGTCSLAYYTNAVNFLVNKIGGNFKVFAFSDDPEWVSSNLKLPVDICFVKN 
                   
               
               
                   
                   
                   
                 AAA076-C03] 
                   
                   
                 NSSEYNYEDLRLMSECNHNIIANSSFSWWGAWLNTNHNKTVITPCKWYADNSTKNADITPSNWIKI 
                   
               
               
                   
               
               
                   Helicobacter   
                 WP_004087499.1 
                 490188900 
                 protein 
                 26.26 
                   
                 MGGGGQDLRLFELMLYNISLPLCFDYKTLVKYFYSNDKSLKYNFPLQYIRYATRSKYHKLYWLALKHYKYFYD 
                 105 
               
               
                   bilis ; 
                   
                   
                 [ Helicobacter   
                   
                   
                 EDPQGDNIVKMYLNNSLEKHAYPFGYFQNLIYFDEIDSIIREEFCLKIPLKPHNQALKEKIEKTENSVFLHVRL 
                   
               
               
                   Helicobacter   
                   
                   
                   bilis ] 
                   
                   
                 GDYLKMEATDGGYVRLGKTYYQSALEILKTRLGQPHIFIFSNDIEWCEKNLCNLLDFTGCHIEFVKANGEGNA 
                   
               
               
                 
                   bilis 
                 
                   
                   
                   
                   
                   
                 AEEMELMRACKHAVIANSTFSWWASYLIDNPDKQIIMPTQVFNDTRRIPKSNMLAKKGYILIDPFWGMHS 
                   
               
               
                 WiWa 
                   
                   
                   
                   
                   
                 IV 
                   
               
               
                   
               
               
                   Ralstonia  sp.  
                 WP_010813809.1 
                 498513378 
                 glycosyl 
                 26.26 
                   
                 MIVTRVIGGLGNQMFQYAAGRALARRLGVPLKIDSSGFADYPLHNYGLHHFALKAVQAGDREIPSGRAEN 
                 106 
               
               
                 GA3-3 
                   
                   
                 transferase 
                   
                   
                 RWAKALRRFGLGTELRVFRERGFAVDPEVMKLPDGTYLDGYWQSESYFAEMTQELRRDFQIATPPTSENA 
                   
               
               
                   
                   
                   
                 family protein 
                   
                   
                 EWLARIGGDEGAVSIHVRRGDYVTNASANAVHGICSLDYYMRAARYVAENIGVKPTFYVFSDDPDWVAG 
                   
               
               
                   
                   
                   
                 [ Ralstonia  sp. 
                   
                   
                 NLHLGHETRYVRHNDSARNYEDLRLMSACRHHIIANSTFSWWGAWLNASEKKVVIAPAQWFRDEKYDTR 
                   
               
               
                   
                   
                   
                 GA3-3] 
                   
                   
                 DLLPPTWTKL 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_004303999.1 
                 490431888 
                 protein 
                 26.25 
                   
                 MVVVYIAAGLANKMFQYAFSRGLMSHGLDVFLDQTSFQPEWSFEDIALEEVFPNIEIKNAPNNMFSLAYKK 
                 107 
               
               
                   ovatus ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 DLLSRIYRRMSAFFPNNRYLMERPFIYDELIYKKATNNCIFCGLWQTELYFNFCERDVRRNFVFTPFQDDQNI 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   ovatus ] 
                   
                   
                 KLAEKMKNENSVAIHIRKGADYLKRNIWDGTCSVEYYNQAINYLKEHVSNPVFYLFTDNPEWVEENLKNID 
                   
               
               
                   ovatus   
                   
                   
                   
                   
                   
                 YKLVDWNPVSGKQSYRDMQLMSCAKHNIIANSTYSWWGAWLNNNPQKIVVAPKIWFNPKIEKAPYIIPD 
                   
               
               
                 3_8_47FAA 
                   
                   
                   
                   
                   
                 RWIRL 
                   
               
               
                   
               
               
                 
                   Loktanella 
                 
                 WP_019955906.1 
                 518799952 
                 protein 
                 26.23 
                   
                 MIITKLIGGLGNQMFQYAAGRSLAMRHGVPLLLDITELRSYPKHQGYQFEDVFAGRFEIAGLIPLIRVLGRKA 
                 108 
               
               
                 
                   vestfoldensis 
                 
                   
                   
                 [ Loktanella   
                   
                   
                 RKVPKTVAVVSPKWPPMGDHVWVRQRTHDYDAAFESIGADCYLSGFWQSEKYFATIAPQIRESFRFKEAL 
                   
               
               
                   
                   
                   
                   vestfoldensis ] 
                   
                   
                 TGANAAIASRMKEAPSAAIHIRRGDYVTDKGAHAFHGLCAWDYYDAAIDHISRHEPDARFFVFSDDVVAA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QERFANRQRAEVVAVNSGRHSYRDMMLMAQCKHQIIANSTFSWWAAWLNQNPDKIVVAPGTWFSGN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DGQIKDIYCKDWIVI 
                   
               
               
                   
               
               
                   Flavobacterium   
                 WP_016991189.1 
                 515558304 
                 protein 
                 26.14 
                   
                 MDVVIIFNGLGNQMSQYAFYSQKKKINNSTYFVPFCKDHNGLELETVFSLNTKETLIQKSLYILFRILLTDRLK 
                 109 
               
               
                 sp. ACAM 123 
                   
                   
                 [ Flavobacterium   
                   
                   
                 IVSDPLKWILNLFKCKIVKESFNYNYNPEYLKPSKGITFYYGGWHAEKYFAKENQQIKSVFEFTGDLGKINKEH 
                   
               
               
                   
                   
                   
                 sp. ACAM 123] 
                   
                   
                 VKDIASTNAVSLHVRRGDFMNEANIGLFGGVSTKAYFEGAIKLIATKVDHPHFFVFSNDMDWVKENLSMD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 TVTYVTCNSGKDSWKDMCLMSLCQHNIIPNSTFSWWGAWLNKNPHKIVVCPSRFLNNDTYTDIYPDSWV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KISDY 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_005779407.1 
                 492219620 
                 glycosyl 
                 26.1 
                   
                 MMKLVRMTGGLGNQMFIYAFYIQMKTIFPELRIDMSEMKKYKLHNGYELEDVFSIRPQTISAHKWLKRVIV 
                 110 
               
               
                   fragilis ;  
                   
                   
                 transferase 
                   
                   
                 YAFFSIIREKSEEELSIHKYTQHKRWPLVYYKGFFQSELFFKESSDTIRDIFSFNTENANFRTKEWAKIIKEQR 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 family 11 
                   
                   
                 SSVSIHIRRGDYTSAKNKIKYGNICTEEYYQKAISIILKKEPKAFFHIFSDDVEWTKAHLKIHHLPHQYISWNK 
                   
               
               
                 
                   fragilis 
                 
                   
                   
                 [ Bacteroides   
                   
                   
                 GPDSWQDMMLMSLCRHNIIANSSFSWWGAWLNAYKDKTVIAPSRWSNVKKTPHILPESWISIDI 
                   
               
               
                 3_1_12 
                   
                   
                   fragilis ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Spirosoma 
                 
                 WP_020598002.1 
                 522086793 
                 protein 
                 26.09 
                   
                 MIISRVTSGLGNQLFQYAAARSLSLRNKTAFYVDLSYYLYEYPDDTSRSFKLGFFSVPYRILQESPVEYLSKST 
                 111 
               
               
                 
                   panaciterrae 
                 
                   
                   
                 [ Spirosoma   
                   
                   
                 KLFPNRSLRPFFLFLKEKQFHFDPTILQAHAGCVIMEGFWQSECYFRDHAEIIRRELQLSKSPSSEFEGYHQQI 
                   
               
               
                   
                   
                   
                   panaciterrae ] 
                   
                   
                 QATPVPVSVHVRRGDYVNHPEFSKTFGFIGLDYYKTAIRHLTKTIKNPHFYVFSDDKEWARANLPLPTDSVFVT 
                   
               
               
                   
                   
                   
                   
                   
                   
                 NTGPSGDVADLVLMSTCHHHIIANSSFSWWGAWLNPNPDKLVITPKLWYKNQPTWNTKDLLPPTWVSL 
                   
               
               
                   
               
               
                 uncultured  
                 EKE06672.1 
                 406985982 
                 glycosyl 
                 26.09 
                   
                 MIITKLTGGLGNQLFQYAIGRNLIYINGSDLKLDVSEYDVSNKGNFRHYALDKFNTIQNFASKKETNNFKFGV 
                 112 
               
               
                 bacterium 
                   
                   
                 transferase 
                   
                   
                 FKKWLYKSGIVKNKNYFLEKKFNFDKEILKIKDNAFLQGYWQSEKYFIGIRDILLQEFSLKENIELKFGEILKE 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 INESNSVSIHVRRGDYVKNPKNLSFHGVCSPKYYSESTSKIASLIEKPVFFVFSDDIEWVKENLNITFPVVYLS 
                   
               
               
                   
                   
                   
                 [uncultured 
                   
                   
                 GIKNIKSYEELVLMSKCKHNIIANSSFSWWGAWLNTNQKKIVIAPKRWFNDVKLDTTDLIPENWIRI 
                   
               
               
                   
                   
                   
                 bacterium] 
                   
                   
                   
                   
               
               
                   
               
               
                 Thermo- 
                 NP_681784.1 
                 22298537 
                 alpha-1,2- 
                 26.07 
                   
                 MIIVHLCGGLGNQMFQYAAGLAAAHRIGSEVKFDTHWFDATCLHQGLELRRVFGLELPEPSSKDLRKVLGA 
                 113 
               
               
                 
                   synechococcus 
                 
                   
                   
                 fucosyl- 
                   
                   
                 CVHPAVRRLLAGHFLHGLRPKSLVIQPHFHYWTGFEHLPDNVYLEGYWQSERYFSNIADIIRQQFRFVEPLD 
                   
               
               
                   elongatus ; 
                   
                   
                 transferase 
                   
                   
                 PHNAALMDEMQSGVSVSLHIRRGDYFNNPQMRRVHGVDLSEYYPAAVATMIEKTNAERFYVFSDDPQW 
                   
               
               
                   Thermo - 
                   
                   
                 [ Thermo - 
                   
                   
                 VLEHLKLPVSYTVVDHNRGAASYRDMQLMSACRHHIIANSTFSWWGAWLNPRPDKVVIAPRHWFNVDV 
                   
               
               
                 
                   synechococcus 
                 
                   
                   
                 
                   synechococcus 
                 
                   
                   
                 FDTRDLYCPGWIVL 
                   
               
               
                 
                   elongatus 
                 
                   
                   
                   elongatus   
                   
                   
                   
                   
               
               
                 BP-1 
                   
                   
                 BP-1] 
                   
                   
                   
                   
               
               
                   
               
               
                   Colwellia   
                 WP_019028421.1 
                 517858213 
                 protein 
                 26.03 
                   
                 MKIVKIAGGFGNQLFQYAFYLALDKKYAEQVCLDSLDMAKYRLHNGYELEGIFKLDARYCTEEQRIIVRKDN 
                 114 
               
               
                 
                   piezophila 
                 
                   
                   
                 [ Colwellia   
                   
                   
                 NIFTKLLSSLKKKLGNNKNYILEPKQEHFTFHEKSFGQANTPTYYKGYWQDVKYLENIEEELKSSLVFPEFELG 
                   
               
               
                   
                   
                   
                   piezophila ] 
                   
                   
                 KNIELANFISSNSSVSLHVRRGDYVQHKAFGGICDLSYYQRAVEQINTLVKDPIFIVFSDDIQWCKDNLNLEK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 AKFVDWNIGENSFRDMQLMTLCKHNIIANSSFSWWGAWLNANDDKNVICPDKWVHYTSATGVLPSEWI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KIKASV 
                   
               
               
                   
               
               
                   Prevotella   
                 WP_019966794.1 
                 518810840 
                 protein 
                 26 
                   
                 MKIVKIIGGLGNQMFQYALAIALQERWKDEEIKLDLHGFNGYHKHQGYQLDMLFGHRFEAATLTDVAQLA 
                 115 
               
               
                 
                   maculosa 
                 
                   
                   
                 [ Prevotella   
                   
                   
                 WPYPHYQLWRVGSRLLPKRRSMLCEPSKGLLPSDVLKQKGSLYYDGYWQDERYFRAIRPQIMAAFKFPDF 
                   
               
               
                   
                   
                   
                   maculosa ] 
                   
                   
                 TDRRNLETEKRLKASEAVSIHVRRGDYLDDVLFQGTCNIAYYQRAIARLCQLKTPVFCIFSNDMAWCKVHIE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PLLHGKEILYVDWNRGKESYRDLQLMTLCRHHIIANSSFSWWGAWLSKAEDGITIAPRHWYAHDAKPSPA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 AERWIKV 
                   
               
               
                   
               
               
                 
                   Salmonella 
                 
                 YP_008261369.1 
                 525860034 
                 fucosyl- 
                 25.99 
                   
                 MYSCLSGGLGNQMFQYAAAYILKQYFQSTTLVLDDSYYYSQPKRDTVRSLELNQFNISYDRFSFADEKEKIKL 
                 116 
               
               
                   enterica ;  
                   
                   
                 transferase 
                   
                   
                 LRKFKRNPFPKQISEILSIALFGKYALSDRAFYTFETIKNIDKACLFSFYQDADLLNKHKQLILPLFELRDDLL 
                   
               
               
                 
                   Salmonella 
                 
                   
                   
                 [ Salmonella   
                   
                   
                 DICKNLELYSLIQRSNNTTALHIRRGDYVTNQHAAKYHGVLDISYYNHAMEYVERERGKQNFIIFSDDVRWAQK 
                   
               
               
                   enterica  subsp. 
                   
                   
                   enterica  subsp. 
                   
                   
                 AFLENDNCYVINNSDYDFSAIDMYLMSLCKNNIIANSTYSWWGAWLNKYEDKLVISPKQWFLGNNETSLR 
                   
               
               
                   enterica  serovar 
                   
                   
                   enterica  serovar 
                   
                   
                 NASWITL 
                   
               
               
                   Worthington  str. 
                   
                   
                   Cubana  str. 
                   
                   
                   
                   
               
               
                 ATCC 
                   
                   
                 CFSAN002050] 
                   
                   
                   
                   
               
               
                 9607;  Salmonella   
                   
                   
                   
                   
                   
                   
                   
               
               
                   enterica  subsp. 
                   
                   
                   
                   
                   
                   
                   
               
               
                   enterica  serovar 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Cubana  str. 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CFSAN001083;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Salmonella 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   enterica  subsp. 
                   
                   
                   
                   
                   
                   
                   
               
               
                   enterica  serovar 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Cubana  str. 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CFSAN002050;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Salmonella 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   enterica  subsp. 
                   
                   
                   
                   
                   
                   
                   
               
               
                   enterica  serovar 
                   
                   
                   
                   
                   
                   
                   
               
               
                   Cubana  str. 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CVM42234 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_008659600.1 
                 495935021 
                 protein 
                 25.94 
                   
                 MKKVIFSGGLGNQMFQYAFYLFLKKKGIKAVIDNSLYSEFKMHNGFELIKVFDIKESIYRTYFLKVHLIFIKLL 
                 117 
               
               
                 3_2_5 
                   
                   
                 [ Bacteroides  sp. 
                   
                   
                 MKIPPVRKLSCKDDVIPIGDHEFDPPYARFYLGYWQSKKIVNYVIEELRAQFIFRNIPQMTIEKGDFLSSINSV 
                   
               
               
                   
                   
                   
                 3_2_5] 
                   
                   
                 SIHIRRGDYMGIPAYQGICNEIYYERAISFMKEHFLNPRFYVFSNDSIWAKLFLEKFDIDMEIIVTPPIYSYWD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 MYLMSRCRNHIIANSTFSWWAAVLNINKDKIVISPTIFKKDECIDIIFDDWVKISNI 
                   
               
               
                   
               
               
                   Clostridium  sp. 
                 WP_022124550.1 
                 547662453 
                 protein 
                 25.86 
                   
                 MIMLQMTGGMGNQMFTYALYRSLRQKGKEVCIEDFTHYDTPEKNCLQTVFHLDYRKADREVYQRLTDSE 
                 118 
               
               
                 CAG: 510 
                   
                   
                 [ Clostridium  sp. 
                   
                   
                 PDFLHKVKRKLTGRKEKIYQEKDAIIFEPEVFQTDDVYMIGYFQSGRYFEKAVFDLRKDFTFAWNTFPEKAKK 
                   
               
               
                   
                   
                   
                 CAG: 510] 
                   
                   
                 LREQMQAESSVSLHIRRGDYMNGKFASIYGNICTDAYYEAARRYMKEHFGDCRFYLFTDDAEWGRQQESE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DTVYVDASEGAGAYVDMALMSCCRHHIIANSSFSWWGAWLDENPDKTVIAPAKWLNISEGKDIYAGLCN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 CLIDANGSVQGE 
                   
               
               
                   
               
               
                 
                   Rhodopirellula 
                 
                 WP_008665459.1 
                 495940880 
                 glycosyl 
                 25.86 
                   
                 MIVTRLIGGLGNQLFQYAFGHSLARSTYQTLLIDDSAFIDYRLHPLAIDHFTISASRLSDADRSRVPGKFLRTP 
                 119 
               
               
                   europaea ;  
                   
                   
                 transferase 
                   
                   
                 VGRALDKVSRFVPGYQGVLPVRREKPFGFRESLLARESDLYLDGYWQSEKFFPGLRGSLREEFQLREQPSETT 
                   
               
               
                   Rhodopirellula   
                   
                   
                 family protein 
                   
                   
                 RRLSAQMKSENSVAIHVRRGDYVTSAKAKQIYRTLDADYYRRCLLDLAAHETDLKLYLFSNDVPWCESNLDV 
                   
               
               
                   europaea  SH398 
                   
                   
                 [ Rhodopirellula   
                   
                   
                 GIPFTPVQHTDGATAHEDLHLIAQCRHVVIANSTFSWWGAYLGQLHPTRRVYYPEPWFHPGTLDGSAMG 
                   
               
               
                   
                   
                   
                   europaea ] 
                   
                   
                 CDDWISEASLEEQSSLKSSRRAA 
                   
               
               
                   
               
               
                 uncultured 
                 EKD23702.1 
                 406873590 
                 glycosyl 
                 25.82 
                   
                 MIIVKLKGGMGNQMFQYAIGRNLATKLGTQLRLDLTFLLDRSPRKDFVFRDYDLDIFALDVAFAGPTDLKPF 
                 120 
               
               
                 bacterium 
                   
                   
                 transferase 
                   
                   
                 TQFRISHLTKIYNIFPRLLGRPYVISEPHFHFSEAILKSSDNVYLDGYWQSEKYFKEIENSIRDDFKFRQPLE 
                   
               
               
                   
                   
                   
                 family protein 
                   
                   
                 GRAAEMAAQIKNEDRAVCLNVRRADFVTSKKAQEFHGFIGLDYYQKAVDLLVSKVGPLHLFIFSDDVDWCAAN 
                   
               
               
                   
                   
                   
                 [uncultured 
                   
                   
                 LKFNYPTTFVTKDYSGKKYEAYLQLMTLCRHYIIPNSTFAWWGAWLNSDPNKIVIAPKQWFKEASIDTTDIIP 
                   
               
               
                   
                   
                   
                 bacterium] 
                   
                   
                 STWIRL 
                   
               
               
                   
               
               
                 
                   Bacillus 
                 
                 WP_000587678.1 
                 446510160 
                 protein  
                 25.74 
                   
                 MIIVKLKGGLGNQMFQYALGKSLALYYDKPLKIDADYIKNNEGYVPRDFSLSKFNIELDLYQEADKERVGFILK 
                 121 
               
               
                   cereus ;  Bacillus   
                   
                   
                 [ Bacillus   
                   
                   
                 NNFLAKKLRNYFLKKGKYKGKYIIENPDNLGLFKKELFENHNESMYIDGYWQSYLYFNNIRECLIKEFNLKPEY 
                   
               
               
                   cereus  AH1271 
                   
                   
                   cereus ] 
                   
                   
                 TKEMTEIMQRINETNSVAVHIRRGDYVKLGWTLDTTYYKKAIAEIVKNVDNPKFYVFSDDTDWVRSNLQEL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DNAVFIGECNLFDYQELWLMSTCKHNIISNSTFSWWGAWLNQNDHQVVVSPSAWINGMSVETTSLIPDS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WKRV 
                   
               
               
                   
               
               
                   Firmicutes   
                 WP_022499937.1 
                 548309386 
                 protein 
                 25.74 
                   
                 MDIIRMEGGLGNQLFQYALYRQLQFMGRTVKMDVTTEYGREHDRQQMLWAFDVHYEEATQEEINRLTD 
                 122 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Firmicutes   
                   
                   
                 GFMDLPSRIRRKLTGRRTKKYAEADSNFDPQVLLKTPVYLTGYFQSEKYFKDVEGILHTELGFSDRIYDGISEV 
                   
               
               
                 CAG: 95 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 FADQIRNYQKQIRETESVSLHVRRGDYLEHPEIYGMSCFMEYYQAGVRYIRERHPDAEIFVFTNDPVFTEKW 
                   
               
               
                   
                   
                   
                 CAG: 95] 
                   
                   
                 LQENFLGDFTLIQGTSEETGYLDLMLMSQCKHQIMANSSFSWWGAWLNPNKDKIVVAPEPWFGDRNFH 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DIYTEEMIRISPRGEVKKHG 
                   
               
               
                   
               
               
                   Prevotella   oris ; 
                 WP_004374901.1 
                 490508875 
                 alpha-1,2- 
                 25.74 
                   
                 MIAATLFGGLGNQMFIYATVKALSLHYQVPMAFNLNHGFANDYKYHRKLELCKFNCQLPTAKWITFDYRG 
                 123 
               
               
                   Prevotella   oris   
                   
                   
                 fucosyl- 
                   
                   
                 ELNIKRISRRIGRNLLCPNYQFVIEEEPFHYEKRLFEFTNKNIFLEGYWQSPCYFENYSKEIRADFQLKVPLSK 
                   
               
               
                 F0302 
                   
                   
                 transferase 
                   
                   
                 EMLEEIYALKATGKTLVMLGIRRYQEVEGRDICTYKLCDKEYYIKAITYIQERIPNALFVVFTQDKEWATTHLP 
                   
               
               
                   
                   
                   
                 [ Prevotella   
                   
                   
                 KGAEFYFVKDKQDEYATVADMFLMTQCTHAIISNSTFYWWGAWLQCFTKNHIVIAPDSFINSDCVCKEWIIL 
                   
               
               
                   
                   
                   
                   oris ] 
                   
                   
                 KRNSLC 
                   
               
               
                   
               
               
                 Escherichia  
                 AAO37719.1 
                 37528734 
                 fucosyl- 
                 25.73 
                   
                 MYSCLSGGLGNQMFQYAAAYILQRKLKQRSLVLDDSYFLDCSNRDTRRRFELNQFNICYDRLTTSKEKKEISII 
                 124 
               
               
                 
                   coli 
                 
                   
                   
                 transferase 
                   
                   
                 RHVNRYRLPLFVTNSIFGVLLKKNYLPEAKFYEFLNNCKLQVKNGYCLFSYFQDATLIDSHRDMILPLFQINED 
                   
               
               
                   
                   
                   
                 [ Escherichia   
                   
                   
                 LLHLCNDLHIYKKVICENANTTSLHIRRGDYITNPHASKFHGVLPMDYYEKAIRYIEDVQGEQVIIVFSDDVK 
                   
               
               
                   
                   
                   
                   coli ] 
                   
                   
                 WAENTFANQPNYYVVNNSECEYSAIDMFLMSKCKNNIIANSTYSWWGAWLNTFEDKIVVSPRKWFAGN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 NKSKLTMDSWINL 
                   
               
               
                   
               
               
                   Leeia   oryzae   
                 WP_018150480.1 
                 516890767 
                 protein  
                 25.71 
                   
                 MIIVKIIGGLGNQMMQYAFAHACAKRLGVPFKLDITAFESYKLWPYGLHNFEITAPIASLEEIEHAKSMGVIT 
                 125 
               
               
                   
                   
                   
                 [ Leeia   
                   
                   
                 ETSFRFDDSLVSAVKDGMYLDGYWADYRYSESVWGELKPVFTLMDPLTPEQQALAMNLSAPNAVALHVR 
                   
               
               
                   
                   
                   
                   oryzae ] 
                   
                   
                 RGDYVTNPNCFLLPQQYYRDAIKLVLDQQPDAVFYCFSDDPDWVEAHLDIPAPKVVVRGQGIDNGFVDMI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 LMSKARHRIVANSTFSIWASRLADQDGLTIVPSQFFRKDDPWLLQVYGEVLQPCYPPQWRVVDVTGDGK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KEAENTSTALLQIAGGDVRGRKLRIGVWGFYEEFYQNNYIFLNKNAPIGHELLKPFNQLYQYGQAHNLEFVT 
                   
               
               
                   
                   
                   
                   
                   
                   
                 LDLVADLSTLDAVLFFDAPNMRSPLVSSVMQLDIKKYLCLLECELIKPDNWQQSLHELFTRIFTWHDGLVDN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 HRYIKVNYVTDLMPWIESAQSLTAPFEETARKGYLQKKLICNISGNKLVSHPFELYSKRIEVIRWFESHHPEH 
                   
               
               
                   
                   
                   
                   
                   
                   
                 FDLYGMGWSASDYPSYKGKIDDKLEVLKGYRFSLCYENAKELPGYITEKIIDCFKAGVVPVYSGAPNIADWIP 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DNCFIDSGKFPDTDALYTYLISMTEEVHADYLENIRQFFLGGKAYPFSADAFINTITRTIVQDCLFPHERTDV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SVVVPNYNHGNFVVSAITSALNQNVSVELLVLDNASTDDSWSQLQFFADYPQVRLIRNRWNIGVQHNWNH 
                   
               
               
                   
                   
                   
                   
                   
                   
                 ATWLATGRYVVMLSADDLLLPGHLEQAVKRLDENPASSLYYTPCLWINEHDQPLGTLNHPGHLESDYVGG 
                   
               
               
                   
                   
                   
                   
                   
                   
                 RDEISDLLKFDSYITPSAAVIRRETLNRIGSMNLHLKGAIDWDLWIRIAEISPAFIFRKQPGVCYRQHSGNNSV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DFYASTAPLEDHIRIVESIIDRKVAVKYLLKAKEEIIAHLDNRASSYPENQIQHLLSRINNIKDYLRKGAGPVI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SVIIPTKNRPGLLANALESLTYQTFKDFEVVIHNDGGCDIGGIVDFFSDQLQISYVRSSQSGGAAASRNRALKL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 AKGRIIAYLDDDDVYLDSHLEKLVDAYKGRSEKFIYTNCEYLIQERKEGRLIELGRERRYAGISYSRAQLLVSN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 FIPTPTWSHTKELIDTIGDFDESLEILEDWDFLLRASKVTEFYQVNATTVEVRSDRSRDDHTLRANADKLLAYH 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QKIYAKHPVENESILANRQSLINSLSNRQDVTPKNENSYQGWVNARQPNELAVQILAERMMLQWSKQYQFMI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VMWKQSQQNLLANTIDSFCQQLYSGWKLIVISDFEAPDESFINNEVLGWLTLETVEDENLLTQAFNGVLA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 EVPSDWVTILPVGTRLTSTALLKVGDRLLLNGGACVIYTDHDYVSDDGMIKDPVLKPAFNLDMLRSQDYIGS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SIFFRTDSLAAVGGFASFPGARTYEACFRMLDNYGPQTIEHLPEPVMTFPENQPENSLRVAAMQLALEEHL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 HRNNISASIEEGYVTGTFLVQYHHSEQPFVSIIIPNKDKHEFLAPCIETLMKVTQYPAFEVIIVDNQSTDPDTL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SYYEEIESRFANNVKVIQYDNPFNFSAQCNLGAESARGDFILFLNNDTEIVQANWLERMMQHAQRNDVGV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VGARLVFPETVTIQHAGIVLGGKYPDEVFQFPYMNFPVDKDVSLNRTKVVQNYSAVTGACLLVRKSLYQQV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 GGMNEQNLAVLYGDVDLCLRIRQLHKSVVWTPFSTLVHHTGKTLNSNSDHEKHLMMVIQTRQEREYMLS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 HWLDIIANDPYYHRLLDKSECNGTIDCTHTPLWDDIPSARPRLQGMALVGGSGEYRVNMPFRTLERSALAE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 IVLSNMTSKARLPSITELARNAPDVFVVQNALADEFIRMLEMYKKYLPSVFRIQMLDDLLTEIPDASSFKRHF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QKNWRDAKARLRKSLKFCDRLIVSTEPLRTFAEDMIDDIIVVPNMLERSVWGDLVSKRRAGKKPRVGWVG 
                   
               
               
                   
                   
                   
                   
                   
                   
                 AQQHAGDLALMTDVVKATGHEVDWVFQGMCPDDIRPYVAEVNTEWLTYDKYPQGIAALNLDLAIAPLEI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 NAFNEAKSNLRLLEYGALGWPVICTDIYPYQTNNAPVCRVPNDASAWIEAIRSHIADLDATAQKGDQLRQ 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WVHDHYMIEDHAQEWLSALTRPAGK 
                   
               
               
                   
               
               
                 
                   Desulfovibrio 
                 
                 WP_005984173.1 
                 492830219 
                 Glycosyl 
                 25.68 
                   
                 MFQYAAARALSLRHSASLAADLTWFSQQFDVQTTPREYALPAFRLNLPEADKRIVATFRLNPTELRIVSFLR 
                 126 
               
               
                   africanus ;  
                   
                   
                 transferase 
                   
                   
                 HRICFPSRFLPRHITELSFDYWDGFRDILPPAYLDGYWQSERYFSDYPDIIRADFSMLSISEQAAWMSAKIAS 
                   
               
               
                 
                   Desulfovibrio 
                 
                   
                   
                 family 11 
                   
                   
                 VQDSISLHIRRGDYVNSLATRKAHGIDTERYYAKALEWIADRIGAATIFAFSDDPRWVRANFDFGKHKGIVV 
                   
               
               
                   africanus  PCS 
                   
                   
                 [ Desulfovibrio   
                   
                   
                 DGSWTAHEDMHLMSLCSHHIIANSSFSWWGAWLSTSQGITIAPKSWFSNPHIWTPDVCPATWERIPC 
                   
               
               
                   
                   
                   
                   africanus ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Akkermansia 
                 
                 WP_022196965.1 
                 547786341 
                 glycosyl 
                 25.66 
                   
                 MAKGKIIVMRLFGGLGNQLFQYAFLFALSRQGGKARLETSSYEHDDKRVCELHHFRVSLPIEGGPPPWAFR 
                 127 
               
               
                   muciniphila   
                   
                   
                 transferase 
                   
                   
                 KSRIPACLRSLFAAPKYPHFREEKRHGFDPGLAAPPRRHTYFKGYFQTEQYFLHCREQLCREFRLKTPLTPEN 
                   
               
               
                 CAG: 154 
                   
                   
                 family 11 
                   
                   
                 ARILEDIRSCCSISLHIRRTDYLSNPYLSPPPLEYYLRSMAEMEGRLRAADAPQESLRYFIFSDDIEWARQNLR 
                   
               
               
                   
                   
                   
                 [ Akkermansia   
                   
                   
                 PALPHVHVDINDGGTGYFDLELMRNCRHHIIANSTFSWWAAWLNEHAEKIVIAPRIWFNREEGDRYHTDD 
                   
               
               
                   
                   
                   
                 
                   muciniphila 
                 
                   
                   
                 ALIPGSWLRI 
                   
               
               
                   
                   
                   
                 CAG: 154] 
                   
                   
                   
                   
               
               
                   
               
               
                   Dysgonomonas   
                 WP_006843524.1 
                 493897667 
                 protein 
                 25.66 
                   
                 MKIVKLQGGLGNQMFQYAIARTLETNKKKDIFLDLSFLRMNNVSTDCFTARDFELSIFPHLRAKKLNSLQEK 
                 128 
               
               
                   mossii ; 
                   
                   
                 [ Dysgonomonas   
                   
                   
                 FLLSDRVRYKFIRKIANINFHKINQLENEIVGIPFGIKNVYLDGFFQSESYFKHIRFDLIKDFEFPELDTRNEA 
                   
               
               
                 
                   Dysgonomonas 
                 
                   
                   
                   mossii ] 
                   
                   
                 LKKTIVNNNSVSIHIRRGDYVHLKNANTYHGVLSLEYYLNCIKRIGEETKEQLSFFIFSDDPEYASKSLSFLPN 
                   
               
               
                   mossii   
                   
                   
                   
                   
                   
                 MQIVDWNLGKNSWKDMALMLACKHHIIANSSFSWWGAWLSERNGITYAPVKWFNNESQYNINNIIPSDWVII 
                   
               
               
                 DSM 22836 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_004372410.1 
                 490506359 
                 glycosyl 
                 25.66 
                   
                 MDIVLIFNGLGNQMSQYAFYMSKKKFVPQSKCMYYKGASNNHNGSELDKLFDIKYSETFFCKLILLLFKLYE 
                 129 
               
               
                   oris ; 
                   
                   
                 transferase, 
                   
                   
                 NIPRLRKYFHILGINIVSEPQNYDYNESILKKKTRFGITLYKGGWHSEKYFLANKQDVLNTFSFKIAKEDKNFI 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                 family 11 
                   
                   
                 DLAKSIEEDTNSVSLHVRRGDYLNISPTDHYQFGGVATTNYYKNAVSYMLKRNKQAHFYIFSDDITWCKAEYK 
                   
               
               
                 
                   oris 
                 
                   
                   
                 [ Prevotella   
                   
                   
                 DLMPTFIECNKKNKSWRDMLLMSLCTNHINANSTFSWWGAWLSTKNGITICPTEFIHNVVTRDIYPETWV 
                   
               
               
                 F0302 
                   
                   
                   oris ] 
                   
                   
                 QL 
                   
               
               
                   
               
               
                 
                   Pseudogul- 
                 
                 WP_008952440.1 
                 496239055 
                 glycosyl 
                 25.66 
                   
                 MIIVRLMGGMGNQLFQYATAFALSKRKSEPLVLDTRFFDHYTLHGGYKLDHFNISARILSKEEESLYPNWQA 
                 130 
               
               
                 
                   benkiania 
                 
                   
                   
                 transferase 
                   
                   
                 NLLLRYPIIDRAFKKWHVERQFTYQDRIYRMKRGQALLGYWQSELYFQEYRKEISAEFTLKEQSSVTAQQISV 
                   
               
               
                 
                   ferrooxidans 
                 
                   
                   
                 family 11 
                   
                   
                 AMQGGNSVAVHIRRGDYLSNPSALRTHGICSLGYYNHAMSLLNERINDAQFYIFSDDIAWAKENIKIGKTSK 
                   
               
               
                 2002;  
                   
                   
                 [ Pseudogul - 
                   
                   
                 NLIFIEGESVETDFWLMTQSKHHIIANSTFSWWGAWLANNTDEQLVICPSPWFDDKNLSETDLIPKSWIRL 
                   
               
               
                 
                   Pseudogul- 
                 
                   
                   
                 
                   benkiania 
                 
                   
                   
                 NKDLPV 
                   
               
               
                 
                   benkiania 
                 
                   
                   
                   ferrooxidans ] 
                   
                   
                   
                   
               
               
                 
                   ferrooxidans 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Salmonella   
                 WP_000286641.1 
                 446208786 
                 protein 
                 25.66 
                   
                 MYSCLSGGLGNQMFQYAAAYILKQYFQSTTLVLDDSYYYSQPKRDTVRSLELNQFNISYDRFSFTDEKEKIKL 
                 131 
               
               
                 
                   enterica 
                 
                   
                   
                 [ Salmonella   
                   
                   
                 LRKFKRNPFPKKISEILSIALFGKYALSDSAFYAVETIKNIDKACLFSFYQDADLLNKHKQLILPLFELRDDLL 
                   
               
               
                   
                   
                   
                   enterica ] 
                   
                   
                 DICKNLDVYPLILRNNNTTALHIRRGDYLTNQHAAKYHGVLDTSYYNNAMEYVERERGKQNFIIFSDDVKWAQ 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KAFLGNENCYIVNNGDYDYSAIDMYLMSLCKNNIIANSTYSWWGAWLNKSEDKLVISPKQWFLGNNETSL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 RNASWIIL 
                   
               
               
                   
               
               
                   Carnobacterium   
                 YP_008718688.1 
                 554649642 
                 glycosyl 
                 25.59 
                   
                 MLIVKVYGGIGNQMFQYSFYKYLQKNNDDVFLDISDYKVHNHHNGFELIDVFNIEVKQADMSKFKGHVSS 
                 132 
               
               
                 sp. WN1359 
                   
                   
                 transferase 
                   
                   
                 KNSIFYRLTSKLFKRNILGYSEFMDSNGISIVRNEKILTDHYFIGFWQDVLYLQSVEEEIKEAFNFKNVAIGK 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 QNLELISLSESVESVSVHIRKGDYANNSDLSDICDLEYYEEAMKIIDSKVSEPLYFIFSDDIEWCKQKFGKRDN 
                   
               
               
                   
                   
                   
                 [ Carnobacterium   
                   
                   
                 LIYVDWNIAKKSYIDMLLMSKCKHNIIANSTFSWWGAWLNNNSKKIVICPKTWDRKKNENHLLLNDWIAI 
                   
               
               
                   
                   
                   
                 sp. WN1359] 
                   
                   
                   
                   
               
               
                   
               
               
                   Prevotella  sp. 
                 WP_021964668.1 
                 547227670 
                 protein 
                 25.58 
                   
                 MMKIIVNMACGLANRMFQYSYYLFLMHKGYNVKVDFYNSAKLAHEKVAWNDIFPKARIEQASFSDILKSG 
                 133 
               
               
                 CAG: 1185 
                   
                   
                 [ Prevotella  sp. 
                   
                   
                 GGSDVISKIRRKYLPFLSSVVNMPTAFDANLPVENKKLQYIIGVFQNANMVEAVEEDVKRCFKFQPFTDERN 
                   
               
               
                   
                   
                   
                 CAG: 1185] 
                   
                   
                 LKLQNEMQSCESVAIHVRKGKDYAQRIWYQNTCPIEYYQNAIRLISEKVNNPKLYVFTDNPEWVKEHFKDF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PYTLVEGNPASGWGSHFDMQLMSVCKHNIISNSTYSWWSAFLNVHNEKIVIGPKVWFNPDSCSEFTSERI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 LCKDWIAV 
                   
               
               
                   
               
               
                   Selenomonas  sp. 
                 WP_009645343.1 
                 497331130 
                 glycosyl- 
                 25.58 
                   
                 MFQYAMASSVARRAGEILKLDLSWIRQMEKKLSADDIYGLGIFSFDEKFSTSNEVQKFLPSGKFSAKIYRAVN 
                 134 
               
               
                 CM52 
                   
                   
                 transferase, 
                   
                   
                 RRMPFSWRRVLEEGGMGWHPQIMEIRRSVYFYMGYWQSEKYFSDFIQEIRKDFTFREEVRQSIEERRPIVE 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 KIRKSDAVSLHIRRGDYAQNPALGEIFLSFTMQYYIDAARYISERVKTPVFFIFSDDIPWAKENLPLPYEVCYI 
                   
               
               
                   
                   
                   
                 [ Selenomonas   
                   
                   
                 DDNIQTNEREIGHKSKGYEDMYLMTQCQHNIIANSSFSWWGAWLNHNPNKIVVAPKKWCNGSFNYADIV 
                   
               
               
                   
                   
                   
                 sp.CM52] 
                   
                   
                 PEQWVKL 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_007486621.1 
                 494751213 
                 protein 
                 25.57 
                   
                 MEIVFIFNGLGNQMSQYALYLSKRNLGCKVRYAYNIRSLSDHNGFELDRVFGITYPNNLFNKCINIIYRLLFAN 
                 135 
               
               
                   nordii ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 KYLFLVQKMIYVLRQMNVYSIKEKDNYDYDYKILTRHKGIVLYYGGWHSEKYFLSNADIIKDKFRFNISKLNSE 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   nordii ] 
                   
                   
                 SLVLYHRLSSLNAVALHVRRGDYMAPEHYNVFGCVCGIEYYKAAIQYIQSQILNPVFIVFSNDIEWVKENITGI 
                   
               
               
                 
                   nordii 
                 
                   
                   
                   
                   
                   
                 QMIFVDFNKKENSWMDMCLMSCCEHNIISNSTFSWWGAWLNNNKNKIVVCPKYFMSNIDTKDIYPESW 
                   
               
               
                 CL02T12C05 
                   
                   
                   
                   
                   
                 IKI 
                   
               
               
                   
               
               
                 
                   Parabacteroides 
                 
                 WP_005635503.1 
                 491855386 
                 protein 
                 25.54 
                   
                 MKKKDIILRVWGGVGNQLFIYAFAKVLSLITDCKVTLDIRTGFANDGYKRVYRLGDFSISLLPALRFYTLLSFA 
                 136 
               
               
                   merdae ;  
                   
                   
                 [ Parabacteroides   
                   
                   
                 QRKMPYIRHLLAYKFDFFEEDQKYPLETLDSFFKIYSDKNLYLQGYWQYFDFSSYRDVLLKDLRFEVEINNTYL 
                   
               
               
                 
                   Parabacteroides 
                 
                   
                   
                   merdae ] 
                   
                   
                 YYSDLIEKSNAVAIHFRRIQYEPVISIDYYKKAIKYISENVENPTFFIFSDDINWCRENLSINGICFFVENFKD 
                   
               
               
                   merdae  ATCC 
                   
                   
                   
                   
                   
                 ELYELKLMSQCNHFIIANSTFSWWGAWLSVNADKKVIMPDGYTDVSMNGSIVHI 
                   
               
               
                 43184; 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Parabacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   merdae 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CL09T00C40 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Butyrivibrio  sp. 
                 WP_022768139.1 
                 551024004 
                 protein 
                 25.51 
                   
                 MIIIQLKGGLGNQMFQYALYKELKHRGRDVKIDDESGFIGDKLRVPVLDRFGVEYDRATKDEVIALTDSKMD 
                 137 
               
               
                 NC2007 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 IFSRIRRKLTGRKTFRIDEMEGIFDPKILETENAYLVGYWQSEKYFTSPEVIEQIQEAFGKRPQEIMHDSVSWS 
                   
               
               
                   
                   
                   
                 sp. NC2007] 
                   
                   
                 TLQQIECCESVSIHVRRTDYMDAEHIKIHNLCSEKYYKNAISKIREEHPNAVFFIFTDDKEWCKEHFKGPKFIT 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VELQEGEFTDVADMLLMSRCKHHIIANSSFSWWSAWLNDSPEKIVIAPSKWINNKKMDDIYTERMTKVAI 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_004302233.1 
                 490430100 
                 protein 
                 25.5 
                   
                 MIVVYSNAGLANRMFHYALYKALEVKGIDVYFDEKSYVPEWSFETTTLMDVFPNIQYRESLQFKRASKKTFL 
                 138 
               
               
                   ovatus ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 DKIVIHCSNLFGGRYYVNYRFKYDDKLFTKLETNQDLCLIGLWQSEKYFMDVRQEIQKCFQYRSFVDDKNVK 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   ovatus ] 
                   
                   
                 TAQQMLSENSVAIHVRKGADYQQNRIWKNTCTIDYYRLAIDYIRMHVQNPVFYVFTDNKDWVIENFTDLD 
                   
               
               
                   ovatus   
                   
                   
                   
                   
                   
                 YTLCDWNPTSGKQNYLDMQLMSCAKHNVIANSTYSWWGAWLNENSDKIVIAPKRWFNKIVTPDILPEQ 
                   
               
               
                 ATCC 8483;  
                   
                   
                   
                   
                   
                 WIKI 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   ovatus   
                   
                   
                   
                   
                   
                   
                   
               
               
                 CL02T12C04 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Mesotoga   prima   
                 YP_006346113.1 
                 389844033 
                 glycosyl 
                 25.5 
                   
                 MRVVWFGGGLGNQMFQYGLYCFLKKNNQEVKADCTQYSTTPMNNGFELERLFNLDIAHANLDVISKLTG 
                 139 
               
               
                 MesG1.Ag.4.2;  
                   
                   
                 transferase 
                   
                   
                 GNRLSPRKVIWKLFRKPKVYFEEKIPFSFDPDVLKGNNRYLKGYWQNMNYLEPCAKELRDVFTFPAFSSDN 
                   
               
               
                   Mesotoga   prima   
                   
                   
                 family protein 
                   
                   
                 NKRLADEIAKVEAVGVHFRRGDFLKSSNLGLFGGICSDQYYLRAIQTMENTVVEPVFYVFCDDPQWAKNSF 
                   
               
               
                   
                   
                   
                 [ Mesotoga   
                   
                   
                 SDARFTVIDWNIGSNSYRDMQLMSLCKHNIIANSTFSWWAAWLNRNPNRTVIAPERMVNRDLDFSGIFP 
                   
               
               
                   
                   
                   
                 
                   prima 
                 
                   
                   
                 NDWIRLQG 
                   
               
               
                   
                   
                   
                 MesG1.Ag.4.2] 
                   
                   
                   
                   
               
               
                   
               
               
                   Clostridium  sp. 
                 WP_021639228.1 
                 545399562 
                 glycosyl- 
                 25.49 
                   
                 MIVLKLQGGLGNQMFEYAFARTIQEQKKDKKLILDTSDFQYDKQREYSLGHFILNENIEIDSSGKFNLWYDQ 
                 140 
               
               
                 KLE 1755 
                   
                   
                 transferase, 
                   
                   
                 RKNPLLKVGFKFWPKFQFQTLKLFGIYVWDYAKYIPVDVSKKHKNILLHGLWQSDKYFSQISEIIRKEFAVKD 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 EPSQGNKAWLERISSANAVCVHIRRGDFLAKGSVLLTCSNSYYLKAMEIISKKVNEPEFFIFSDDIEDVKKIFE 
                   
               
               
                   
                   
                   
                 [ Clostridium  sp. 
                   
                   
                 FPGYQITLVNQSNPDYEELRLMSKCKHFIIANSTFSWWSSLLSENEDKVIVAPRLWYSDGRDTSALMRDEWII 
                   
               
               
                   
                   
                   
                 KLE 1755] 
                   
                   
                 IDNE 
                   
               
               
                   
               
               
                   Bacteroides   
                 WP_022052991.1 
                 547321746 
                 glycosyl- 
                 25.42 
                   
                 MDLVTLSGGLGNQMFQFAFYWALKKRGKKVFLYKNKLAAKEHNGYELQTLFGVEEKCVDGLWMTRLLGC 
                 141 
               
               
                 
                   plebeius 
                 
                   
                   
                 transferase 
                   
                   
                 PLLGKILKHILFPHKIRERVLYNYSIYLPLFERNGLHWVGYWQSEKYFQDVADDIRRIFCFDHLSLNPATSAA 
                   
               
               
                 CAG: 211 
                   
                   
                 family 11 
                   
                   
                 LKCMSEQVAVSVHIRRGDYYLPCNVATYGGLCTVEYYENAIRYVKERYPQAVFYVFSDDLDWVRENIPSAGK 
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                 MVFVDWNRGKDSWQDMFLMSKCHHNILANSSFSWWGAWLNTHPEKLVIAPERWANCPAPDALPDG 
                   
               
               
                   
                   
                   
                 
                   plebeius 
                 
                   
                   
                 WVRIEGVSRR 
                   
               
               
                   
                   
                   
                 CAG: 211] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Treponema 
                 
                 WP_021686002.1 
                 545448980 
                 glycosyl- 
                 25.4 
                   
                 MAIKIVKISGGLGNQMFCYAFACALQKCGHKVYVDTSLYRKATVHSGIDFCHNGLETERLFGIKFDEADTAD 
                 142 
               
               
                   lecithinolyticum ;  
                   
                   
                 transferase, 
                   
                   
                 VRRLSTSAEGLLNRIRRKYFTKKTHYIDTVFKYTPELLSDKNDCYLEGYWQTEKYFLPIEKDIRRLFTFRPTL 
                   
               
               
                 
                   Treponema 
                 
                   
                   
                 family 11 
                   
                   
                 SEKSAAVQSALQAQQAAVLSASIHVRRGDFLNTKTLNVCTETYYNNAIKYAVKKHAVSRFYIFSDDIPWCREH 
                   
               
               
                 
                   lecithinolyticum 
                 
                   
                   
                 [ Treponema   
                   
                   
                 LCFCNAHAVFIDWNTGNDSWQDMALMSMCRCNIIANSSFSWWAAWLNNASDKTVLAPAIWNRRQLEYV 
                   
               
               
                 ATCC 700332 
                   
                   
                   lecithinolyticum ] 
                   
                   
                 DRYYGYDYSDIVPESWIRIPID 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_004291980.1 
                 490419682 
                 glycosyl 
                 25.34 
                   
                 MRLIKMTGGLGNQMFIYAFYLRMKKRHTNTRIDLSDMMHYNVHHGYEMHRVFNLPKTEFCINQPLKKVI 
                 143 
               
               
                   eggerthii ;  
                   
                   
                 transferase 
                   
                   
                 EFLFFKKIYERKQDPSSLLPFDKKYLWPLLYFKGFYQSERFFADMENDIRIAFTFNSDLFNEKTQAMLTQIKH 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 [ Bacteroides   
                   
                   
                 NEHAVSLHIRRGDYLEPKHWKTTGSVCQLPYYLNAITEMNKRIEQPSYYVFSDDIAWVKENLPLPQAVFIDW 
                   
               
               
                 
                   eggerthii 
                 
                   
                   
                   eggerthii ] 
                   
                   
                 NKGAESWQDMMLMSHCRHHIICNSTFSWWGAWLNPRENKTVIMPERWFQHCDTPNIYPDGWIKVPVN 
                   
               
               
                 DSM 20697 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_005656005.1 
                 491891563 
                 glycosyl 
                 25.34 
                   
                 MRFIKMTGGLGNQMFIYAFYMRMKKHYSNTRIDLSDMVHYKAHNGYEMHRVFNLPPIEFRINQPLKKVIE 
                 144 
               
               
                   stercoris ;  
                   
                   
                 transferase 
                   
                   
                 FLFFKKIYERKQVPSSLVPYDKKYFWPLLYFKGFYQSERFFADMADDIRKAFTFNPRLSNRKTKEMSEQIDHD 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 [ Bacteroides   
                   
                   
                 ENAVSIHVRRGDYLEPKYWKTTGCVCQLPYYLNAIAEMNKRISQPSYYVFSDDIAWVKENLPLPKAFFIDW 
                   
               
               
                   stercoris   
                   
                   
                   stercoris ] 
                   
                   
                 NKGAESWQDMMLMSRCRHHIICNSTFSWWGAWLNPRENKTVIMPERWFRHCETPDICPDKWIKVPIN 
                   
               
               
                 ATCC 43183 
                   
                   
                   
                   
                   
                 QPDSIQ 
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 YP_003831842.1 
                 302671882 
                 glycosyl 
                 25.34 
                   
                 MIIIQLKGGMGNQMFQYALYRQLKKLGREVKIDDETGFVDDELRIPVLQRFGISYDKATREEIVKLTDSKMD 
                 145 
               
               
                   proteoclasticus ;  
                   
                   
                 transferase 11 
                   
                   
                 IFSRIRRKLTGRKTFRIDEESGIFDPRILEVEDAYLVGYWQSDKYFANEEVEKEIREAFEKRPQEVMQDSVSW 
                   
               
               
                 
                   Butyrivibrio 
                 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 TILQQIECCESVSLHIRRTDYIDEEHIHIHNICTEKYYKSAIDEVRNQYPSAVFFIFTDDKDWCRQHFRGPNF 
                   
               
               
                   proteoclasticus   
                   
                   
                 
                   proteoclasticus 
                 
                   
                   
                 FVVDLDEDTNTDIAEMTLMSRCKHHILANSSFSWWAAWLNDNPGKIVIAPSKWINNRKMDDIYTARMKKIAI 
                   
               
               
                 B316 
                   
                   
                 B316] 
                   
                   
                   
                   
               
               
                   
               
               
                   Roseobacter  sp. 
                 WP_008228724.1 
                 495504071 
                 alpha-1,2- 
                 25.34 
                   
                 MSPIVHFPSDRLLRYEHLNSLWKTAMIYTRLLARLGNQMFQYAAGRGLAARLGVDFTVDSRRAVHKGDGV 
                 146 
               
               
                 GAI101 
                   
                   
                 fucosyl- 
                   
                   
                 LTRVFDLDWAAPENMPPAQHERPLAYYAWRGLRRDPKIYRENGLGYNAAFETLPDNTYLHGYWQCERYF 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 AHIADDIRAAFVPRHPMSAQNADMARRIASGPSVSLHVRRGDYLTVGAHGICDQTYYDAALAAVMQGLP 
                   
               
               
                   
                   
                   
                 [ Roseobacter   
                   
                   
                 SPTVYVFSDDPQWAKDNLPLTFEKVVVDFNGPDSDYEDMRLMSLCQHNVIANSSFSWWGAWLNANPQ 
                   
               
               
                   
                   
                   
                 sp. GAI101] 
                   
                   
                 KRVAGPANWFSNPKLSNPDILPSRWIRI 
                   
               
               
                   
               
               
                 
                   Thalassobacter 
                 
                 WP_021099615.1 
                 544666256 
                 alpha-1,2- 
                 25.34 
                   
                 MGQDMIYSRIFGGLGNQLFQYATARAVSLRQGVELVLDTRLAPPGSHWAFGLDHFNISARIAEPSELPPSK 
                 147 
               
               
                   arenae ;  
                   
                   
                 fucosyl- 
                   
                   
                 DNFFKYVMWRAFGHDPAFMRERGLGYQSRIAQAPDGTYLHGYFQSERYFADVLDHLENELRIVTPPDTRN 
                   
               
               
                 
                   Thalassobacter 
                 
                   
                   
                 transferase, 
                   
                   
                 AEYADRIASAGHTVSLHVRRGDYVETSKSNSTHATCDEAYYLRALARLSEGKSDLKVFVFSDDPEWVRDNLK 
                   
               
               
                   arenae   
                   
                   
                 [ Thalassobacter   
                   
                   
                 LPYDTTPVGHNGPDKPHEDLRLMSCCSDHVIANSTFSWWGGWLDRRPEARVVGPAKWFNNPKLVNPDI 
                   
               
               
                 DSM 19593 
                   
                   
                   arenae ] 
                   
                   
                 LPERWIAI 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_004377401.1 
                 490511493 
                 protein 
                 25.33 
                   
                 MKIIKIIGGLGNQMFQYALAVALQKKWKDEEIKLDLHGFNGYHKHQGYQLDEIFGHRFKAASLKEVAQLA 
                 148 
               
               
                   oris ;  Prevotella   
                   
                   
                 [ Prevotella   
                   
                   
                 WPYPHYQLWRVGSRLLPKRKTMVCESADCRFQSDLLNLEGSLYYDGYWQDERYFKAFRTEIIEAFKFTPLV 
                   
               
               
                   oris  C735 
                   
                   
                   oris ] 
                   
                   
                 GDSNRKVENMLKEGRFASLHVRRGDYLKEPLFQSTCDIAYYQRAISRLNQMADPYCYLIFSNDIAWCKTHIE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PLCDGRRTHYVDWNHGKESYRDMQLMTFCKHHIIANSSFSWWGAWLSTANDGITIAPHQWYANDRKP 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SPAAEAWLKL 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_004380180.1 
                 490514606 
                 protein 
                 25.33 
                   
                 MKIVRIIGGLGNQMFQYALALALKQQQENEEVKLDLSAFRGYKKHGGFQLVQCFGTTLPAATWQEVAQL 
                 149 
               
               
                   oulorum ;  
                   
                   
                 [ Prevotella   
                   
                   
                 AWYYPHYQLWRLGHRVLPVCKTMLKEPDNGAFLPEVLQRKGDAYYEGCWQDERYFSHYRPAILQAFTFP 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                   oulorum ] 
                   
                   
                 TFTNPRNLAMQQQINTTESVAIHVRRGDYLHDALFRNTCGLAYFQRAITCILQHVAHPVFYVFSDDMAWC 
                   
               
               
                   oulorum  F0390 
                   
                   
                   
                   
                   
                 RQHIQPLLQTNEAVFVDWNHGKASICDLHLMTLCRHHIIANSSFSWWGAWLSPHQAGWIIAPKQWYAH 
                   
               
               
                   
                   
                   
                   
                   
                   
                 EEKMSPAAERWLKL 
                   
               
               
                   
               
               
                 
                   Spirosoma 
                 
                 WP_020596174.1 
                 522084965 
                 protein 
                 25.33 
                   
                 MNRRVAVQLKGGLGNQLFQYALGRRLSLQLEAELLFDCSVLENRIPVTNFTFRSFDLDMFRIAGRVATPSDL 
                 150 
               
               
                 
                   panaciterrae 
                 
                   
                   
                 [ Spirosoma   
                   
                   
                 PLFPKSASIRSPWPHLVQLARLWKQGYSYVYERGFAYNPKMLRQLSDRVYLNGYWQSYRYFEDIAATLRAD 
                   
               
               
                   
                   
                   
                   panaciterrae ] 
                   
                   
                 CSFPDPLPDSAVGLAGQINATNSICLHIRRTDFLQVPLHQVSNADYVGRAIAYMAERVNDPHFFVFSDDIA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WCQTNLRLSYPVVFVPNELAGPKNSLHFRLMRYCKHFITANSTFSWWAAWLSEPSDGKVIVTPQTWFSDS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 RSIDDLIPANWIRL 
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 YP_003829826.1 
                 302669866 
                 glycosyl 
                 25.26 
                   
                 MNYVEVKGGLGNQLFQYTFYKYLEKKSGHKVLLHTDFFKNIDSFEEATKRKLGLDRFDCDFVAVSGFISCEKL 
                 151 
               
               
                   proteoclasticus ;  
                   
                   
                 transferase 11 
                   
                   
                 VKESDYKDSMLSQDEVFYSGYWQNKRFFLEVMDDIRKDLLLKDENIQDEVKELAKELRAVDSVAIHFRRGD 
                   
               
               
                 
                   Butyrivibrio 
                 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 YLSEQNKKIFTSLSVDYYQKAIAQLAERNGADLKGYIFTDEPEYVSGIIDQLGSIDIKLMPVREDYEDLYLMSC 
                   
               
               
                 
                   proteoclasticus 
                 
                   
                   
                 
                   proteoclasticus 
                 
                   
                   
                 ARHHIIANSSFSWWGAALGDTESGITIAPAKWYVDGRTPDLYLRNWISI 
                   
               
               
                 B316 
                   
                   
                 B316] 
                   
                   
                   
                   
               
               
                   
               
               
                   Butyrivibrio  sp. 
                 WP_022765786.1 
                 551021623 
                 protein 
                 25.26 
                   
                 MIIIQLKGGLGNQMFQYALYKELKHRGREVKIDDVSGFVNDKLRVPVLDRFGVEYERATREEVVELTDSRM 
                 152 
               
               
                 XPD2006 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 DIFSRIRRKLTGRKTYRIDEMEGIFDPAILETENAYLVGYWQSEKYFTSPEVIEQIQEAFGKRPQEIMHDSVS 
                   
               
               
                   
                   
                   
                 sp. XPD2006] 
                   
                   
                 WSTLQQIECCESVSIHVRRTDYVDAEHIKIHNLCSEKYYKNAIGKIREKHPNAVFFIFTDDKEWCKDHFKGPN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 FITVELQEGEFTDVADMLLMSRCKHHIIANSSFSWWSAWLNDSPEKMVIAPSKWINNKKMDDIYTERMT 
                   
               
               
                   
                   
                   
                   
                   
                   
                 RVAI 
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_008766093.1 
                 496041586 
                 protein 
                 25.24 
                   
                 MKIVNITGGLGNQMFQYAFAMALKYRNPQEEVFVDIQHYNTIFFKKFKGINLHNGYEIDKVFPKAKLPVAG 
                 153 
               
               
                 1_1_6 
                   
                   
                 [ Bacteroides  sp. 
                   
                   
                 VRQLMKFSYWIPNYILSRLGRKFLPIRKKEYIPPYSMNYSYDEKALNWKGDGYFEGYWQSYNHFGDIKEELQ 
                   
               
               
                   
                   
                   
                 1_1_6] 
                   
                   
                 KVYAHPKPNQYNAALISNLESCNSVGIHVRRGDYLAEPEFRGICGLDYYEKGIKEILSDEKKYVFFIFSNDMQ 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WCQENIAPLVGDNRIVFISGNKGKDSCWDMFLMTHCKDLIIANSSFSWWGAFLNKKVDRVICPKPWLNR 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DCNIDIYNPSWILVPCYSEDW 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 YP_099857.1 
                 53713865 
                 alpha-1,2- 
                 25.17 
                   
                 MKIVTFQGGLGNQLFQYVFYLWLDMRCDKDNIYGYYPKKGLRAHNGLEIEKVFEVKLPNSSLSTDLIVKSIKL 
                 154 
               
               
                   fragilis ;  
                   
                   
                 fucosyl- 
                   
                   
                 INKIFKNRQYISTDGRLDVNGVLFEGFWQDKYFWEDVDIVLNFRWPLKLDVTNSFIMTKIQANNSISIHIRR 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 transferase 
                   
                   
                 GDYLLPKYRNIYGDICNEEYYQKAIEYILKCVDDPFFFVFSDDIDWAKSIINVSNVTFVNNNKGKDSYIDMFL 
                   
               
               
                   fragilis   
                   
                   
                 [ Bacteroides   
                   
                   
                 MSLCHHNIIANSTFSWWAAQLNKHSDKIMIAPIRWFKSLFKDPNIFTESWIRI 
                   
               
               
                 YCH46 
                   
                   
                   fragilis   
                   
                   
                   
                   
               
               
                   
                   
                   
                 YCH46] 
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_008671843.1 
                 495947264 
                 alpha-1,2- 
                 25.17 
                   
                 MIKIVSFSGGLGNQLFQYLLVVYLRECGHQVYGYYNRKWLIGHNGLEVNNVFDIYLPKTNFIVNALVKVIRV 
                 155 
               
               
                 9_1_42FAA 
                   
                   
                 fucosyl- 
                   
                   
                 LRCLGFKKYVATDTYNNPIAIFYDGYWQDQKYFNIIDSKLSFKKFDLSAENKSILSKIKSNISVALHIRCGDYL 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 SSSNVEIYGGVCTKEYYEKALELVCKIKNVMFFVFSDDIEYAKLLLNLPNAIYVNANVGNSSFIDMYLMANCKV 
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                 NVIANSTFSYWAARLNQDNILTIYPKKWYNSKYAVPDIFPSEWVGV 
                   
               
               
                   
                   
                   
                 sp. 9_1_42FAA] 
                   
                   
                   
                   
               
               
                   
               
               
                   Coraliomargarita   
                 WP_022477844.1 
                 548260617 
                 glycosyl 
                 25.17 
                   
                 MIIVKVQGGLGNQMFQYAFGRALSEKHSQDLYLDCSEYLRPSCKREYGLDHFNIRAKKASCGDVKSMVTP 
                 156 
               
               
                 sp. CAG: 312 
                   
                   
                 transferase 
                   
                   
                 HFALRKKLKKIFAVPYSLSPTHILERNFNFQPSILEFNCGYFDGFWQTQKYFSGISDIVRKDLTFKDAVKYSG 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 GETFAKITSLNSVSLHIRRGDYVKVKRTRKRFSVIRAGYFKRAVEYMRSKLDTPHFFIFTDDPKWVSENFPAG 
                   
               
               
                   
                   
                   
                 [ Coraliomargarita   
                   
                   
                 EDYTLVSSSGMYEDLFLMAQCRHNIIFNSSFSWWGAWLNGNPGKIVVAPDMWFTPHYKLDYSDVVPEEWI 
                   
               
               
                   
                   
                   
                 sp. CAG: 312] 
                   
                   
                 KLNTGYFESKEF 
                   
               
               
                   
               
               
                 
                   Pseudorhodobacter 
                 
                 WP_022705649.1 
                 550957292 
                 alpha-1,2- 
                 25.17 
                   
                 MIVMQIKGGLGNQMFQYAAGRALSLQTGMPLHLDLRYYRREREHGYGLGAFNIEASPLDESLLPPLPRESP 
                 157 
               
               
                 
                   ferrugineus 
                 
                   
                   
                 fucosyl- 
                   
                   
                 LAWLIWRLGRRGPNLVRENGMGFNPTLSNVTKPAWITGYFQSERYFAAHAATIRAELTPVAAPDLVNAR 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 WLAEIAAEPRAVSLHVRRGDYVRDAKAAAKHGSCTPAYYERALAHITARMGTAPVVYAFSDDPAWVRENL 
                   
               
               
                   
                   
                   
                 [ Pseudo - 
                   
                   
                 RLPAEIRVPGHNDTAGNVEDLRLMSACRHHIVANSSFSWWGAWLNPRADKIVASPARWFADPAFTNPDI 
                   
               
               
                   
                   
                   
                 
                   rhodobacter 
                 
                   
                   
                 WPEAWARIEG 
                   
               
               
                   
                   
                   
                   ferrugineus ] 
                   
                   
                   
                   
               
               
                   
               
               
                   Escherichia   coli ; 
                 YP_002329683.1 
                 215487252 
                 fucosyl- 
                 25.16 
                   
                 MMYCCLSGGLGNQMFQYAAAYILKQHFPDTILVLDDSYYFNQPQKDTIRHLELDQFKIIFDRFSSKDEKVKI 
                 158 
               
               
                   Escherichia   coli   
                   
                   
                 transferase 
                   
                   
                 NRLRKHKKIPLLNSFLQFTAIKLCNKYSLNDASYYNPESIKNIDVACLFSFYQDSKLLNEHRDLILPLFEIRD 
                   
               
               
                 O127: H6 str. 
                   
                   
                 [ Escherichia   
                   
                   
                 DLRVLCHNLQIYSLITDSKNITSIHVRRGDYVNNKHAAKFHGTLSMDYYISAMEYIESECGSQTFIIFTDDVI 
                   
               
               
                 E2348/69 
                   
                   
                   coli  O127:  
                   
                   
                 WAKEKFSKYSNCLVADADENKFSVIDMYLMSLCNNNIIANSTYSWWGAWLNRSEDKLVIAPKQWYISGNECSL 
                   
               
               
                   
                   
                   
                 H6 str. 
                   
                   
                 KNENWIAM 
                   
               
               
                   
                   
                   
                 E2348/69] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_016359991.1 
                 511537894 
                 protein 
                 25.16 
                   
                 MIIIKVMGGLGNQMQQYALYEKFKSIGKNVKLDISWFEDSSVQEKVFARRSLELRQFKDLQFDTCSAEEKEA 
                 159 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 LLGKSGILGKLERKLIPARNKHFYESDIYHSEVFNMSDAYLEGHWACEKYYHDIMPLLQEKIQFPESANSQNI 
                   
               
               
                 3_1_57FAA_CT1 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 TVKKRMKAENSVSIHIRRGDYLDPENEAMFGGICTNSYYKAAEEYIKSRVPDTHFYLFSDDTAYLRENYHGD 
                   
               
               
                   
                   
                   
                 3_1_57FAA_CT1] 
                   
                   
                 EYTIVDWNKGEDSFYDMELMSCCRHNICANSTFSFWGARLNRTPDKIVIRPAKHKNSQEIEPQLLHELWD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 NWVIIDGDGRIV 
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 WP_022755397.1 
                 551010878 
                 glycosyl 
                 25.09 
                   
                 MKPLVSLIVPVLNVEKYLEQCLTSISSQTYDNFEVILVVGKCIDNSENICKKWCEKDHRFRIEPQLKSCLGYA 
                 160 
               
               
                 
                   fibrisolvens 
                 
                   
                   
                 transferase 
                   
                   
                 RNVGIDAAKGEYIAFCDSDDCITSDFLSCFVDTALKNSSDIVETQFTLCDQNLSPIYDYDRNILGHILGHGFL 
                   
               
               
                   
                   
                   
                 [ Butyrivibrio   
                   
                   
                 EYTSAPSVWKYFVKRDIFTSNNLHYPEIRFGEDISMYSLLFSYCNKIDYVEKPTYLYRQVPSSLMNNPQGKRK 
                   
               
               
                   
                   
                   
                   fibrisolvens ] 
                   
                   
                 RYESLFDHDFVTNEFKTRLLFQKSWLKLLFQLEMHSASIISDSATSDDEAISMRQEISGYLKKVFPVKNTIFE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VTALGWGGEIVSSIASKFNTLHGVSSSNMFNRYFFELLEDSTRKKLEEMIINFSPDIFLIDLISEADYLSSYK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 GNLGTFVKNWKIGFSIFMKMIQTHSNNSSIFLLENYMQQAPDHVDNTNEILKMLYDDIKINHPDIICISPAPD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 ILNRSSEPELPCIYQLKLVSDKLHTMYSPVINCVETKGGLGNQIFQYVFSKYIEKMTGYRPLLHIGFFDYVKA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 IPGGTKRIFSLDKLFPDIETTSGKIPCSHVVEEKSFISNPGSDIFYRGYWQDIRYFSDVKDEVLESFNVDTSS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 MSKDVIDFADTIRNANSIAMHIRRQDYLNENNVSLFEQLSIDYYKSAVDMIRKEYADDLVLFIFSDDPEYANS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 IADSFDIEGFVMPLHKDYEDLYLITLAHHHIIANSTFSLWGALLSARKDGIRIAPRNWFKGTPATNLYPDKWL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 IL 
                   
               
               
                   
               
               
                 
                   Anaeromusa 
                 
                 WP_018702959.1 
                 517532751 
                 protein 
                 25.08 
                   
                 MFCVRIYGGLGNQMFQYALGRAMAKHYSETAAFDLSWYEQKIKPGFEASVCQYNIELSRKDRPKAWYEPI 
                 161 
               
               
                 
                   acidaminophila 
                 
                   
                   
                 [ Anaeromusa   
                   
                   
                 LKRISRHTDKLEMWFGLFFEKKYHYDSTVFERGLCKKNITLDGYWQSYKYFSAIEDDLRRELTIPKEREELI 
                   
               
               
                   
                   
                   
                   acidaminophila ] 
                   
                   
                 AISRSLPENSVSIHVRRGDYVSNPKANAMHGTCSWEYYQAAIEKMTGLVKEPQYVVFSDDITWTKENLPLPN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 AMYIGRELGLFDYEELILMSRCKHNIMANSTFSWWGAWLNSNPNKVVIAPRKWFRHKKIKVNDLFPSSWV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VL 
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_008768986.1 
                 496044479 
                 glycosyl 
                 25.08 
                   
                 MDIVVIFNGLGNQMSQYAFYLAKKKDNLNCHVIFDPKSTNVHNGAELKRVFGIELNRNYLDKIISYFYGYIFN 
                 162 
               
               
                 2_1_16 
                   
                   
                 transferase 
                   
                   
                 KRIVNKLFSLVGIRMIYEPKNYDYREELLKPSSNFISFYWGGWHSEKYFKDIELEVKKVFKFPEVTNSPYFTEW 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 FNKIFLDNNSVSIHIRRGDYLDKPSDPYYQFNGVCTIDYYEKAILYLKERILEPNFYIFSNDINWCMKTFGTEN 
                   
               
               
                   
                   
                   
                 [ Bacteroides  sp. 
                   
                   
                 MYYVDCNKGKDSWRDMYLMSECRHHINANSTFSWWAAWLSPYSNGIVLHPKYFIKDIETKDYYPQKWI 
                   
               
               
                   
                   
                   
                 2_1_16] 
                   
                   
                 MIE 
                   
               
               
                   
               
               
                 
                   Chlorobium 
                 
                 YP_001960319.1 
                 189500849 
                 glycosyl 
                 25.08 
                   
                 MDKVVVHLTGGLGNQMFQYALGRSISINRNCPLLLNTSFYDTYDKFSCGLSRYNVKAEFIKKNSYYNNKYYR 
                 163 
               
               
                   phaeobacteroides ; 
                   
                   
                 transferase 
                   
                   
                 YVIRLLSRYGVACYFGSYYEKKIFSYDEKVYKRSCVSYYGTWQSYGYFDSIRDILLRDYEMVGCLEEEVEKYVS 
                   
               
               
                 
                   Chlorobium 
                 
                   
                   
                 family protein 
                   
                   
                 DIKRVDSVSLHIRRGDYFDNKRLQSIHGILTMEYYYKAMSLFPDSSVFYVFSDDIEWVRENLITNTNIVYVVLE 
                   
               
               
                 
                   phaeobacteroides 
                 
                   
                   
                 [ Chlorobium   
                   
                   
                 SDNPENEIYLMSLCKNNIISNSTFSWWGAWLNKNKYKKVIAPRMWYKDNQSSSDLMPSDWCLI 
                   
               
               
                 BS1 
                   
                   
                 
                   phaeobacteroides 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 BS1] 
                   
                   
                   
                   
               
               
                   
               
               
                   Treponema   
                 WP_022932606.1 
                 551312724 
                 protein 
                 25.08 
                   
                 MIVISMGGGLGNQMFEYAFYTQLKHLYPKSEIKVDTKYAFPYSHNGIEVFKIFGLNPPEANWKEVHSLVKTY 
                 164 
               
               
                 
                   bryantii 
                 
                   
                   
                 [ Treponema   
                   
                   
                 PIEGNKAHFIKFFLYRILRKANLVEREPTSFCKQKDFTEFYNSFFELPQNKSFYLYGPFVNYNYFAAIHNEIMD 
                   
               
               
                   
                   
                   
                   bryantii ] 
                   
                   
                 LYTFPEITDVTNIEYKRKIESSHSISIHIRRGDYITEGVPLVPDAYYREALVYINKKIEDPHFFVFTDDKDYCK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SLFSDNQNFTIVEGNTGANSFRDMQLMSLCKHNIIANSTFSFWGAFLNKNSEKIVIAPNIAFKDCSCPYICPDW 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 YP_005110943.1 
                 375358171 
                 LPS  
                 25 
                   
                 IILMVIAKLFGGLGNQMFIYAAAKGIAQISNQKLTFDIYTGFEDDSRFRRVYELKQFNLSVQESRRWMSFRYPL 
                 165 
               
               
                   fragilis ;  
                   
                   
                 biosynthesis 
                   
                   
                 GRILRKISRKIGFCIPLVNFKFIVEKKPYHFQNEIMRIASFSSIYLEGYFQSYKYFSKIEAQIREDFKFTKEVI 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 alpha-1,2- 
                   
                   
                 GSVEKEASFITNSRYTPVAIGVRRYSEMKGEFGELAVVEHDYYDAAIKYIANKVPNLIFIVFSEDIDWVKKNLK 
                   
               
               
                   fragilis  638R 
                   
                   
                 fucosyl- 
                   
                   
                 LDYPVYFVTSKKGELAAIQDMYLMSLCNHHIISNSSFYWWGAYLASTNNHIVIAPSVFLNKDCTPIDWVII 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                   
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                   
                   
               
               
                   
                   
                   
                   fragilis  638R] 
                   
                   
                   
                   
               
               
                   
               
               
                   Firmicutes   
                 WP_022352105.1 
                 547951298 
                 protein 
                 25 
                   
                 MSGGLGNQMFQYALYMKLTAMGREVKFDDINEYRGEKAWPIMLAVFGIEYPRATWDEIVAFTDGSMDFSK 
                 166 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Firmicutes   
                   
                   
                 RLKRLFRGRHPIEYVEQGFYDPKVLSFENMYLKGSFQSQRYFEDILEEVQETFRFPELKDMNLPAPLYETT 
                   
               
               
                 CAG: 534 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 EKYLLRIEGCNAVGLHMYRGDSRSNEELYDGICTEKYYEGAVRFIQDKCPDAKFFIFSNEPKWVKGWVISLM 
                   
               
               
                   
                   
                   
                 CAG: 534] 
                   
                   
                 KSQIREDMSREEIRALEDHFVLIENNTEYTGYLDMFLMSRCRHNIISNSSFSWWAAFINENPDKLVTAPSRW 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VNGVPSEDVYVKGMTLIDEKGRVERTIKE 
                   
               
               
                   
               
               
                   Firmicutes   
                 WP_022368748.1 
                 547971670 
                 glycosyl 
                 25 
                   
                 MVIVKIGDGLGNQMFNYVCGYSVAKHDNDTLLLDTSDVDNSTLRTYDLDKFNIDFTDRESFTNKGFFHKVY 
                 167 
               
               
                 
                   bacterium 
                 
                   
                   
                 transferase 
                   
                   
                 KRLRRSLKYNVIYESRTENCPCVLDVYRRKFIRDKYLHGYFQNLCYFKTCKEDIMRQFTPKEPFSAKADELIHR 
                   
               
               
                 CAG: 882 
                   
                   
                 family 11 
                   
                   
                 FATENTCSVHVRGGDIKPLSIKYYKDALDKIGEAKKDMRFIVFSNVRNLAEEYIKELGVDAEFIWDLGEFTDIE 
                   
               
               
                   
                   
                   
                 [ Firmicutes   
                   
                   
                 ELFLMKACRRHILSDSTFSRWAALLDEKSEEVFVPFSPDADKIYMPEWIMEEYDGNEEKR 
                   
               
               
                   
                   
                   
                 
                   bacterium 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 CAG: 882] 
                   
                   
                   
                   
               
               
                   
               
               
                 Vibrio 
                 WP_005496882.1 
                 491639353 
                 glycosyl 
                 25 
                   
                 MVIVKVSGGLGNQLFQYAIGCAISNRLSCELLLDTSFYPKQSLRKYELDKFNIKAKVATQKEVFSCGGGDDLL 
                 168 
               
               
                   parahaemolyticus ;  
                   
                   
                 transferase 
                   
                   
                 SRFLRKLNLSSLFFPNYIKEKESLVYLAEISHCKSGSFLDGYWQNPQYFSDIKDELVKQIVPIMPLSSPALEWQ 
                   
               
               
                   Vibrio   
                   
                   
                 family 11  
                   
                   
                 NIIINTKNCVSLHVRRGDYVNNAHTNSVHGVCDLSYYREAITNIHETVEKPKFFVFSDDISWCKDNLGSLGHF 
                   
               
               
                 
                   parahaemolyticus 
                 
                   
                   
                 [ Vibrio   
                   
                   
                 TYVDNTLSAIDDLMLMSFCEHHIIANSTFSWWGAWLNDHGITIAPKRWFSSVERNNKDLFPEKWLIL 
                   
               
               
                 10329;  Vibrio   
                   
                   
                 
                   parahaemo- 
                 
                   
                   
                   
                   
               
               
                 
                   parahaemolyticus 
                 
                   
                   
                   lyticus ] 
                   
                   
                   
                   
               
               
                 10296;  Vibrio   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   parahaemolyticus 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 12310;  Vibrio   
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   parahaemolyticus 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 10290 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Herbaspirillum 
                 
                 WP_006463714.1 
                 493509348 
                 glycosyl 
                 24.92 
                   
                 MIVSRLIGGLGNQMFQYAAGRALALRRGVPFAIDSRAFADYKTHAFGMQCFCADQTEAPSRLLPNPPAEG 
                 169 
               
               
                   frisingense ;  
                   
                   
                 transferase 
                   
                   
                 RLQRLLRRFLPNPLRVYTEKTFTFDEAVLSLPDGIYLDGYWQSEKYFADFADDIRKDFAVKAAPSAPNQAWL 
                   
               
               
                 
                   Herbaspirillum 
                 
                   
                   
                 family protein 
                   
                   
                 ELIGRTHSVSLHIRRGDYVSNAAAAAVHGTCDLGYYERAVAHLHQVTGQAPELFVFSDDLDWVATNLQLP 
                   
               
               
                 
                   frisingense 
                 
                   
                   
                 [ Herbaspirillum   
                   
                   
                 YTMHLVRDNDAATNFEDLRLMTACRHHIVANSSFSWWGAWLDGRSESITIAPARWFVADTPDARDLVP 
                   
               
               
                 GSF30 
                   
                   
                   frisingense ] 
                   
                   
                 QRWVRL 
                   
               
               
                   
               
               
                   Rhizobium  sp.  
                 WP_007759661.1 
                 495034125 
                 Glycosyl 
                 24.92 
                   
                 MIITRILGGLGNQMFQYAAGRALAIANEAELKLDLIEMGAYKLRPFALDQFNIKAAIAQPDEVPAKPKRGLL 
                 170 
               
               
                 CF080 
                   
                   
                 transferase 
                   
                   
                 RKFTSAFKPDRSSCERIVENGLTFDSRVPALRGSLHLSGYWQSEQYFASSADAIRSDFSLKSPLGPARQDVLA 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 RIGAATTPVSIHVRRGDYVTNPSANAVHGTCEPPWYHEAMRRMLDRAGDASFFVFSDEPQWARDNLQS 
                   
               
               
                   
                   
                   
                 [ Rhizobium  sp. 
                   
                   
                 SRPMVFIEPQNNGRDGEDMHLMAACHAHIIANSSFSWWGAWLNPRPNKHVIAPRQWFRAPDKDDRDI 
                   
               
               
                   
                   
                   
                 CF080] 
                   
                   
                 VPATWERL 
                   
               
               
                   
               
               
                 
                   Verrucomicrobium 
                 
                 WP_009959380.1 
                 497645196 
                 glycosyl 
                 24.85 
                   
                 MVISHISEGLGNQMFQYAAGRRLSYHLGTTLKLDDYHYRLHPFRSFQLDRFLITSPIATDAEISHLCPLEGLAR 
                 171 
               
               
                 
                   spinosum 
                 
                   
                   
                 transferase, 
                   
                   
                 AIRARLPGKLRGATLRLLGNLGLGSPYQPRLHSFKEETPKQPLLIGKVVSERHFHFDPDVLECPDNVCLVGY 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 WQDERYFGEIRDILLRELTLKSPPAGATKAVLERIQRSSSVSLHVRRGDKTKSSSYHCFSLEYCLAAMSEMRA 
                   
               
               
                   
                   
                   
                 [ Verrucomicrobium   
                   
                   
                 RLQAPTFFVFSDDWDWVREQIPCSSSVIHVDHNRAEDVSEDFRLMKSCDHHIIASSSLSWWAAWLGTNE 
                   
               
               
                   
                   
                   
                   spinosum ] 
                   
                   
                 NSFVFSPPADRWLNFSNHFTADVLPPHWIQLDGSSLLPAQ 
                   
               
               
                   
               
               
                 
                   Fibrella 
                 
                 YP_007319049.1 
                 436833833 
                 glycosyl 
                 24.83 
                   
                 MTANRVLVNSPMVIAKITSGLGNQLFQYALGRHLALQGNTSLWFDLRYFHQEYATDTPRKFKLDRFNVRY 
                 172 
               
               
                   aestuarina ;  
                   
                   
                 transferase 
                   
                   
                 NLLDSSPWLYASKATRLLPGRSLRPLIDTRFEADFHFDPTVIRPAAPLTILWGFWQSEKYFAQSTPQIRQELTF 
                   
               
               
                 
                   Fibrella 
                 
                   
                   
                 family 11 
                   
                   
                 NRPLSDTFVGYQQQIEQAEVPISVHVRRGDYVTHPEFSQSFGFVGLAYYQKALAHLQDLFPNATLFFFSDDP 
                   
               
               
                   aestuarina   
                   
                   
                 [ Fibrella   
                   
                   
                 DWVRANIVTEQPHVFVQNSGPDADVDDLQLMSLCHHHVIANSSFSWWGAWLNPRPDKVVIGPQRWF 
                   
               
               
                 BUZ 2 
                   
                   
                   aestuarina   
                   
                   
                 ANKPWDTKDLLPSGWLRL 
                   
               
               
                   
                   
                   
                 BUZ 2] 
                   
                   
                   
                   
               
               
                   
               
               
                   Rhodobacter  sp. 
                 WP_023665745.1 
                 563380195 
                 alpha-1,2- 
                 24.83 
                   
                 MIHMRLVGGLGNQLFQYACGRAVALRHGTELVLDTRELSRGAAHAVFGLDHFAIRARMGASADLPPPRS 
                 173 
               
               
                 CACIA14H1 
                   
                   
                 fucosyl- 
                   
                   
                 RVLAYGLWRAGFMAPRFLRERGLGVNPAVLAAGDGTYLHGYFQSEAYFRDVVPQIRPELEIVTPPSDDNLR 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 WASRIAGDDRAVSLHVRRGDYVASAKGQQVHGTCDADYYARAVAAIRARAGIDPRLYVFSDDPHWARD 
                   
               
               
                   
                   
                   
                 [ Rhodobacter   
                   
                   
                 NLALDAETVVLDHNPPGAAVEDMRLMGVCRHHIIANSSFSWWGAWRNPSAGKVVVAPVRWFADPKLH 
                   
               
               
                   
                   
                   
                 sp. CACIA14H1] 
                   
                   
                 NPDICPPEWLRV 
                   
               
               
                   
               
               
                   Rhodopirellula   
                 NP_868779.1 
                 32475785 
                 fucosyl 
                 24.83 
                   
                 MATSAHLHLSDEKQTLDSKASDRDCATTEASASDKTCTISISGGLGNQMLQYAAGRALSIHHDCSLQLDLKF 
                 174 
               
               
                 
                   baltica 
                 
                   
                   
                 transferase 
                   
                   
                 YSSKRHRSYELDAFPIQAHRSIKPSFFSQILSKIQSESKHVPTYQEQSKRFDPAFFNTEPPVKIRGYFFSEKYF 
                   
               
               
                 SH 1;  
                   
                   
                 [ Rhodopirellula   
                   
                   
                 SPYADQIRTELTPPIPPDQPARDMAIRLKECVSTSLHVRRGDYVTNANARQRFWCCTSEYFEAAIERLPTDSTV 
                   
               
               
                 
                   Rhodopirellula 
                 
                   
                   
                   baltica  SH 1] 
                   
                   
                 FVFSDDIEWAKQNIRSSRTTVYVNDELKKAGSPETGLRDLWLMTHAKSHIIANSSFSWWGAWLANSEANL 
                   
               
               
                 
                   baltica 
                 
                   
                   
                   
                   
                   
                 TIAPKKWFNDPEIDDSDIVPSSWHRI 
                   
               
               
                   
               
               
                 
                   Spirosoma 
                 
                 WP_020604054.1 
                 522092845 
                 protein 
                 24.83 
                   
                 MVVVELMGGLGNQMFQYAFGMQLAHQRQDTLTVSTFLLSNKLLANLRNYTYRPFELCIFGIDKPKASPFN 
                 175 
               
               
                 
                   spitsbergense 
                 
                   
                   
                 [ Spirosoma   
                   
                   
                 LLRALLPFDLNTSLLRETDDPEAVIPAASARIVCVGYWQSEHYFEEVTVHVREKFIFRQPFNSFTSRLANNLN 
                   
               
               
                   
                   
                   
                   spitsbergense ] 
                   
                   
                 GIPNSVFVHIRRGDYVTNKGANAHHGLCDRTYYERAVTFMREHLENPLFFIFSDDLEWVSQELGPILEPATY 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VGGNQKNDSWQDMYLMSLCRHAIVANSSFSWWGAWLSPHASKIVVAPKEWFGKPLLPVKTNDLIPNS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WIRI 
                   
               
               
                   
               
               
                 uncultured  
                 EKD71402.1 
                 406938106 
                 protein 
                 24.76 
                   
                 MNAIIPRLTGGIGNQLFIYAAARRMAIANSMNLVIDDTSGFKYDVLYKRFYQLEKFNITSRMATPTERLEPFS 
                 176 
               
               
                 bacterium 
                   
                   
                 ACD_46C00193 
                   
                   
                 KIRRYLKRKINKTYPFAQRAYITQEKSGFDPRLLVFRPKGNVYLDGYWQSENYFKDIEGIIRQDLIIKSPSDS 
                   
               
               
                   
                   
                   
                 G0003 
                   
                   
                 LNIATAERIKNTLAIAVHVRFFDMVDISDSSNCQSNYYHTAIAKMEEKIPNAHYFIFSDKPVLARLAMPLPDD 
                   
               
               
                   
                   
                   
                 [uncultured 
                   
                   
                 RITIIDHNIGDMNAYADLWLMSLCKHFVIANSTFSWWGAWLSDNKEKIVIAPDIKITSGVTQWGFDGLIPDEW 
                   
               
               
                   
                   
                   
                 bacterium] 
                   
                   
                 IKL 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_006950883.1 
                 494008437 
                 protein 
                 24.75 
                   
                 MDVIVIFNGLGNQMSQYAFYLEKRLRNRQTTYFVLNPRSTYELERLFGIPYRSNLMCRMIYKLLDKAYFSNHI 
                 177 
               
               
                   micans ;  
                   
                   
                 [ Prevotella   
                   
                   
                 RLKKILRTALNAVGIRLIVEPITRNYSLSNFTHHPGLTFYRGGWHSELNFTSVVTELRRKFIFPPSDDEEFKRI 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                   micans ] 
                   
                   
                 SALIIRTQSISLHIRRGDYLDYSEYQGVCTEEYYERAIEYIRSHVENPVFFVFSDDKEYAINKFSGDDSFRIVD 
                   
               
               
                   micans  F0438 
                   
                   
                   
                   
                   
                 FNTGENSWRDMQLMSLCRHHILANSTFSWWGAWLDSAPEKIVLHPIYHMRDVPTRDFYPHNWIGISGE 
                   
               
               
                   
               
               
                   Thermo - 
                 AHB87954.1 
                 564737556 
                 alpha-1,2- 
                 24.75 
                   
                 MIIVRLYGGLGNQMFQYAAGLALSLRHAVPLRFDLDWFDGVRLHQGLELHRVFDLDLPRAAPSEMRQVL 
                 178 
               
               
                 
                   synechococcus 
                 
                   
                   
                 fucosyl- 
                   
                   
                 GSFSHPLVRRLLVRRRLRWLLPQGYALEPHFHYWPGFEALGPKAYLDGYWQSERYFSEYQDAVRAAFRFA 
                   
               
               
                 sp. NK55 
                   
                   
                 transferase 
                   
                   
                 QPLDERNRQIVEEMAACESVSLHVRRGDFVQDPVVRRVHGVDLSAYYPRAVALLMERMREPRFYVFSDD 
                   
               
               
                   
                   
                   
                 [ Thermo - 
                   
                   
                 PDWVRANLKLPAPMIVIDHNRGEHSFRDMQLMSACRHHILANSSFSWWGAWLNSQPHKLVIAPKRWF 
                   
               
               
                   
                   
                   
                 
                   synechococcus 
                 
                   
                   
                 NVDDFDTRDLYCSGWTVL 
                   
               
               
                   
                   
                   
                 sp. NK55] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Coleofasciculus 
                 
                 WP_006100814.1 
                 493031416 
                 Glycosyl 
                 24.73 
                   
                 MLSLNKNFLFVHIPKSCILKEVYIYMISFPNLGKGVRLGNQMFQYAFLRSTARRLGVKFYCPAWSGDSLFTLN 
                 179 
               
               
                   chthonoplastes ;  
                   
                   
                 transferase 
                   
                   
                 DQEERVSQPEGITKQYRQGLNPGFSENALSIQDGTEISGYFQSDKYYDNPDLVRQWFSLKEEKIASIRDRFSR 
                   
               
               
                 
                   Coleofasciculus 
                 
                   
                   
                 family 11 
                   
                   
                 LNFANSVGMHLRFGDVVGQLKRPPMRRSYYKKALSYIPNQELILVFSDEPERTKKMLDGLSGNFLFLSGHK 
                   
               
               
                   chthonoplastes   
                   
                   
                 [ Coleofasciculus   
                   
                   
                 NYEDLYLMTKCQHFICSYSTFSWWGAWLGGERERTVIYPKEGQYRPGYGRKAEGVSCESWIEVQSLRGFL 
                   
               
               
                 PCC 7420 
                   
                   
                   chthonoplastes ] 
                   
                   
                 DDYRLVSRLEKRLPKSLMNFFY 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_018666797.1 
                 517496220 
                 glycosyl 
                 24.66 
                   
                 MRLIKMTGGLGNQMFIYAFYLRMKKRHTNTRIDLSDMMHYNVHHGYEMHHVFNLPKTEFCINQPLKKVI 
                 180 
               
               
                 gallinarum 
                   
                   
                 transferase 
                   
                   
                 EFLFFKKIYERKQDSSNLLPFDKKYFWPLLYFKGFYQSERFFADMENDIRKAFTFNSGLFNEKTQTMLKQIEH 
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                 NEHAVSLHVRRGDYLEPKHWKTTGSVCQLPYYINAIAEMNRRIEQPFYYVFSDDIAWVKENLPLPQAVFID 
                   
               
               
                   
                   
                   
                   gallinarum ] 
                   
                   
                 WNKGVESWQDMMLMSHCRHHIICNSTFSWWGAWLNPKENKTVIMPERWFQHCETPNIYPAGWIKVP 
                   
               
               
                   
                   
                   
                   
                   
                   
                 IN 
                   
               
               
                   
               
               
                   Firmicutes   
                 WP_022367483.1 
                 547967507 
                 glycosyl 
                 24.66 
                   
                 MNNVEIMGGLGKQLFQYAFSRYLQKLGVKNVVLRKDFFTIQFPENNGITKREFVLDKYNTRYVAAAGEKTY 
                 181 
               
               
                 
                   bacterium 
                 
                   
                   
                 transferase 
                   
                   
                 RDYCDENDYRDDYAIGSDEVLYEGYWQNIDFYNVVRKEMQEELKLKPEFIDNSMAAVEKDMSSCNSVALH 
                   
               
               
                 CAG: 882 
                   
                   
                 GT11 family 
                   
                   
                 IRRSDYLTQVNAQIFEQLTQDYYASAVSIIEQYTHEKPVLYIFSDDPEYAAENMKDFMGCRTVIMPPCEPYQ 
                   
               
               
                   
                   
                   
                 [ Firmicutes   
                   
                   
                 DMYLMTRAKHNIIANSTFSWWGATLNANPDNITVAPSRWMKGRTVNLYHKDWITL 
                   
               
               
                   
                   
                   
                 
                   bacterium 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 CAG: 882] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_008021494.1 
                 495296741 
                 protein 
                 24.6 
                   
                 MIAVNVNAGLANQMFHYAFGRGLMAKGLDVCFDQSNFKPRSQWAFELVRLQDAFPSIDIKVMPEGHFK 
                 182 
               
               
                   xylanisolvens ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 WVFPSLPRNGLERRFQEFMKKWHNFIGDEVYIDEPMYGYVPDMEKCATRNCIYKGFWQSEKYFRHCEDD 
                   
               
               
                   Bacteroides   
                   
                   
                   xylanisolvens ] 
                   
                   
                 IRKQFTFLPFDELKNIEVAAKMSQENSVAIHLRKGDDYMQSELMGKGLCTVDYYMKAIDYMRKHINNPHF 
                   
               
               
                 
                   xylanisolvens 
                 
                   
                   
                   
                   
                   
                 YVFTDNPCWVKDNLPEFEYILVDWNEVSGKRNFRDMQLMSCAKHNIIGNSTYSWWAAWLNANQDKIVV 
                   
               
               
                 CL03T12C04 
                   
                   
                   
                   
                   
                 GPKRFFNPINSFFSTCDIMCEDWISL 
                   
               
               
                   
               
               
                   Geobacter  sp.  
                 YP_004197726.1 
                 322418503 
                 glycosyl 
                 24.58 
                   
                 MIGMVIFRAYNGLGNQMFQYALGRHLALLNEAELKIDTTAFADDPLREYELHRLKVQGSIATPDEIAFFRE 
                 183 
               
               
                 M18 
                   
                   
                 transferase 
                   
                   
                 MENTHPQAYLRLTQKSRLFDPAILSARGNIYLHGFWQTEKYFADIREILLDEFEPIVPAGEDSIKVLSHMK 
                   
               
               
                   
                   
                   
                 family protein 
                   
                   
                 ATNAVALHVRRSDYVSNPMTLRHHGVLPLDYYREAVRRIAGMVPDPVFFIFSDDPQWAKDNIRLEYPAFCV 
                   
               
               
                   
                   
                   
                 [ Geobacter  sp. 
                   
                   
                 DAHDASNGHEDLRLMRNCKHFIIANSSFSWWGAWLSQNTGKKVVAPLKWFAKPEIDTRDIVPLQWIRI 
                   
               
               
                   
                   
                   
                 M18] 
                   
                   
                   
                   
               
               
                   
               
               
                   Ruegeria   
                 YP_168587.1 
                 56698215 
                 alpha-1,2- 
                 24.57 
                   
                 MITTRLHGRLGNQMFQYAAARGLAARLGTQVALDTRLAESRGEGVLTRVFDLDLAQPDQLPPLKGDGLLR 
                 184 
               
               
                 
                   pomeroyi 
                 
                   
                   
                 fucosyl- 
                   
                   
                 HGAWRLLGLAPRFRREHGLGYNAAIETWDDGTYLHGYWQSERYFAHIAARIRADFAFPAFSNSQNAEMA 
                   
               
               
                 DSS-3 
                   
                   
                 transferase, 
                   
                   
                 ARIGDTDAISLHVRRGDYVALAAHTLCDQRYYAAALTRLLEGVAGDPVVYLFSDDPAWARDNLALPVQKV 
                   
               
               
                   
                   
                   
                 [ Ruegeria   
                   
                   
                 VVDFNGPETDFEDMRLMSLCRHNIIGNSSFSWWAAWLNAHPGKRIAAPASWFGDAKLHNPDLLPPDWL 
                   
               
               
                   
                   
                   
                   pomeroyi   
                   
                   
                 KIEV 
                   
               
               
                   
                   
                   
                 DSS-3] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_016291997.1 
                 511037973 
                 protein 
                 24.52 
                   
                 MIIIQLAGGLGNQMQQYAMYQKLLSLGKKVKLDISWFEEKNRQKNVYARRELELNYFKKAEYEACFEEERK 
                 185 
               
               
                   bacterium  28-4 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 ALVGEGGFAGKIKGKLFPGTRKIFRETEMYHPEIFDFEDRYLYGYFACEKYYADIMEILQEQFVFPPSGNPEN 
                   
               
               
                   
                   
                   
                   bacterium  28- 
                   
                   
                 QKMAERIADGESVSLHIRRGDYLDAENMAMFGNICFEEYYAGAIREMKKIYPSAHFFVFSDDIPYAKETYSG 
                   
               
               
                   
                   
                   
                 4] 
                   
                   
                 EEFTVVDINRGKDSFFDIWLMSGCRHNICANSTFSFWGARLNRNKGKVVMRPFIHKNSQKFEPELMHEL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WKGWVFIDNRGNIC 
                   
               
               
                   
               
               
                   Prevotella  sp. 
                 WP_021989703.1 
                 547254188 
                 glycosyl 
                 24.49 
                   
                 MRILVFTGGLGNQMFEYAFYKHLKSCFPKESFYGHYGVKLKEHYGLEINKWFDVTLPPAKWWTLPVVGLFY 
                 186 
               
               
                 CAG: 1092 
                   
                   
                 transferase 
                   
                   
                 LYKKLVPNSKWLDLFQREWKHKDAKVFFPFKFTKQYFPKENGWLKWKVDEASLCEKNKKLLQVIHDEETCF 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 VHVRRGDYLASNFKSIFEGCCTLDYYKRALEYMNKNNPKVRFICFSDDLEWMRKNLPMDDSAIYVDWNTG 
                   
               
               
                   
                   
                   
                 [ Prevotella  sp. 
                   
                   
                 TDSPLDMYMMSQCDNGIIANSSFSYWGAYLGGKKTTVIYPQKWWNMEGGNPNIFMDEWLGM 
                   
               
               
                   
                   
                   
                 CAG: 1092] 
                   
                   
                   
                   
               
               
                   
               
               
                   Spirosoma   luteum   
                 WP_018618567.1 
                 517447743 
                 protein 
                 24.41 
                   
                 MVISVLSGGLGNQLFQYAFGLKLAAQLQTELRLERHLLESKAIARLRQYTPRTYELDTFGVEAPAASLMDTVS 
                 187 
               
               
                   
                   
                   
                 [ Spirosoma   
                   
                   
                 CLSRVALSDKTALLLRESTLTPNAINNLNNRVRDVVCLGYWQSEEYFRPATEQLRKHLVFRKNPAQSRSMA 
                   
               
               
                   
                   
                   
                   luteum ] 
                   
                   
                 DTILSCQNAAFVHIRRGDYVTNTHANQHHGLCDVSYYRRACEYVKECIPDVQFFVFSDDPDWAKRELGIHL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QPARFIDHNRGADSWQDMYLMSLCRHAIVANSSFSWWGAWLNPVAERLVVAPGQWFVNQPVLSQQII 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PPHWHCL 
                   
               
               
                   
               
               
                 
                   Marinomonas 
                 
                 YP_004480472.1 
                 333906886 
                 glycosyl 
                 24.34 
                   
                 MIIVDLSGGLGNQMFQYACARSLSIELNLPLKVVYGSLASQTVHNGYELNRVFGLDLEFATENDMQKNLGF 
                 188 
               
               
                   posidonica   
                   
                   
                 transferase 
                   
                   
                 FLSKPILRKIFSKKPLNNLKFQNFFPENSFNYNSSLFSYIKDSGFLQGYWQTEKYFLNHKSQILKDFCFVNMD 
                   
               
               
                 IVIA-Po- 
                   
                   
                 family protein 
                   
                   
                 DETNISIANDIQSGHSISIHVRRGDYLTNLKAKAIHGHCSLDYYLKAIEFLQEKIGESRLFIFSDDPEWVSEN 
                   
               
               
                 181; 
                   
                   
                 [ Marinomonas   
                   
                   
                 IATRFSDVSVIQHNRGVKSFNDMRLMSMCDHHIIANSSFSWWGAWLNPSQNKKIIAPKNWFVTDKMNTIDLIP 
                   
               
               
                 
                   Marinomonas 
                 
                   
                   
                   posidonica   
                   
                   
                 SSWILK 
                   
               
               
                 
                   posidonica 
                 
                   
                   
                 IVIA-Po-181] 
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides ;  
                 WP_005839979.1 
                 492425792 
                 glycosyl 
                 24.32 
                   
                 MKIVVFKGGLGNQLFQYAFYKYLSRKDETFYFYNDAWYNVSHNGFELDKYFKTDDLKKCSRFWIILFKTILSK 
                 189 
               
               
                   Bacteroides  sp. 
                   
                   
                 transferase 
                   
                   
                 LYHWKIYVVGSVEYQYPNHLFQAGYFLDKKYYDENTIDFKHLLLSEKNQSLLKDIQNSNSVGVHIRRGDYMT 
                   
               
               
                 4_3_47FAA;  
                   
                   
                 family 11 
                   
                   
                 KQNLVIFGNICFQKYYHDAIRIITEKVNDAVFYVFSDDISWVQTHLDIPNAVYVNWNTGESSIYDMYLMSSC 
                   
               
               
                   Bacteroides  sp. 
                   
                   
                 [ Bacteroides ] 
                   
                   
                 KYNIIANSTFSYWAARLNKKTNMVIYPSKWYNTFTPDIFPESWCGI 
                   
               
               
                 3_l_40A;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   dorei 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 5136/D4;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   vulgatus 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 PC510;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   dorei 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CL03T12C01;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   vulgatus  dnLKV7 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Candidatus 
                 
                 WP_020169431.1 
                 519013556 
                 protein 
                 24.32 
                   
                 MTIRIKLTGGLGNQMFQFATGFAIAKKKNVRLSLDLKYINKRKLFNGFELQKIFNIYSKVSFLNKTLSFKSI 
                 190 
               
               
                   Pelagibacter   
                   
                   
                 [ Candidatus   
                   
                   
                 NFTEILNRIDTTFYNFKEPHFHYTSNILNLPKHSFLDGYWQSELYFNEFATEIKRIFNFSGKLDKSNLLVAD 
                   
               
               
                 
                   ubique 
                 
                   
                   
                 
                   Pelagibacter 
                 
                   
                   
                 DINRNNSISIHIRRGDFLLKQNNNHHTDLKEYYLKAINETSKIFKNPKYFIFSDDTSWTVDNFVIDHPYIIV 
                   
               
               
                   
                   
                   
                   ubique ] 
                   
                   
                 DINFGARSFLDMYLMSLCKSNIIANSSFSWWSAWLNNNKDKIIYAPKNWFNDKSICTDDLIPESWNIIL 
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_022353174.1 
                 547952428 
                 uncharacterized 
                 24.29 
                   
                 MSVIINMACGLANRMFQYAFYLYLQKEGYDAYVDYFTRADLVHENVDWLRIFPEATFRRATARDIRKMGG 
                 191 
               
               
                 CAG: 875 
                   
                   
                 protein 
                   
                   
                 GHDCFSRLRRKLLPMTTKVLETSGAFEIILPPKNRDSYLLGAFQSAKMVESVDAEVRRIFTFPEFESGKNQY 
                   
               
               
                   
                   
                   
                 [ Bacteroides  sp. 
                   
                   
                 FQTRLAQENSVGLHIRKGKDYQERIWYKNTCGVEYYRKAVDLMKEKVDSPSFYVFTDNPAWVKENLSWLEY 
                   
               
               
                   
                   
                   
                 CAG: 875] 
                   
                   
                 KLVDGNPGSGWGSHCDMQLMSLCKHNIISNSSYSWWGAYLNNTLNKIVVCPRIWFNPESTKDFSSNPLLA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 EGWISL 
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 WP_022756327.1 
                 551011911 
                 protein 
                 24.29 
                   
                 MIIIKLQGGLGNQLFLYGLYKNLKHLKRDVKMDIESGFEGDELRKPCLDCMNLEYAIATRDEVTDIRDSYMDI 
                 192 
               
               
                 
                   fibrisolvens 
                 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 FSRIRRKITGRKTFDYYEPEDGNYDPKVLEMTKAYLNGYFQSEKYFGDEESVKALKDELTKGKEDILTSTDLIT 
                   
               
               
                   
                   
                   
                   fibrisolvens ] 
                   
                   
                 KIYHDIKNSESVSLHIRRGDYLTPGIIETYGGICTDEYYDKAIAMIRETFPEARFFIFSNDIEWCKEKFAGDKN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 ILFVNTIGINLDSEDNIKIGKSDKDISEYRDLAELYLMSACKHHILANSSFSWWGAWLSDHEGMTIAPSKWLNN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KNMTDIYTKDMLLI 
                   
               
               
                   
               
               
                 
                   Roseburia 
                 
                 YP_004839455.1 
                 347532692 
                 glycosyl 
                 24.22 
                   
                 MVTVKIGDGMGNQMYNYACGYAAAKRSGEKLRLDISECDNSTLRDYELDHFRVVYDEKESFPNRTFWQK 
                 193 
               
               
                   hominis ;  
                   
                   
                 transferase 
                   
                   
                 LYKRLRRDIRYHVIRERDMYAVDARVFVPARRGRYLHGYWQCLGYFEEYLDDLREMFTPAYEQTDAVREL 
                   
               
               
                 
                   Roseburia 
                 
                   
                   
                 family protein 
                   
                   
                 MQQFTQTPTCALHVRGGDLGGPNRAYFQQAIARMQKEKPDVTFIVFTNDLPKAKECLDDGEARMRYIAE 
                   
               
               
                   hominis   
                   
                   
                 [ Roseburia   
                   
                   
                 FGEALSDIDEFFLMSACQNQIISNSTYSTWAAYLNTLPGRIVIVPKFHGVEQMALPDWIVLDGGACQKGEID 
                   
               
               
                 A2-183 
                   
                   
                   hominis  A2-183] 
                   
                   
                 AV 
                   
               
               
                   
               
               
                 
                   Rhodopirellula 
                 
                 WP_008659200.1 
                 495934621 
                 alpha-1,2- 
                 24.16 
                   
                 MATSVHPHLSDGKQALDSKAAQQVCSTQAASASDRACTISISGGLGNQMLQYAAGRALSIHHDCPLQLDL 
                 194 
               
               
                   europaea ;  
                   
                   
                 fucosyl- 
                   
                   
                 KFYSSKRHRSYELDAFPIQAQRWIKPSFFSQVLDKIQGESKSAPTYEEQSKRFDRAFFDIELPARIRGYFFSE 
                   
               
               
                 
                   Rhodopirellula 
                 
                   
                   
                 transferase 
                   
                   
                 KYFLPYADQIRTELTPPVPLDQPARDMAQRLSEGMSTSLHVRRGDYVSNANARQRFWSCTSEYFEAAIEQMP 
                   
               
               
                   europaea  6C 
                   
                   
                 [ Rhodopirellula   
                   
                   
                 ADSTVFVFSDDIEWAKQNIRSSRPTVYVNDELKLAGSPETGLRDLWLMTHAKSHIIANSSFSWWGAWLSG 
                   
               
               
                   
                   
                   
                   europaea ] 
                   
                   
                 SEANLTIAPKKWFNDPEIDDSDIVPTSWRRI 
                   
               
               
                   
               
               
                   Rudanella   lutea   
                 WP_019988573.1 
                 518832653 
                 protein 
                 24.16 
                   
                 MVIAKITSGLGNQLFQYALGRHLAIQNQTRLWFDLRYYHRTYETDTPRQFKLDRFSIDYDLLDYSPWLYVSK 
                 195 
               
               
                   
                   
                   
                 [ Rudanella   
                   
                   
                 ATRLLPGRSLRPLFDTRKEPHFHLDPAVPNAKGAFITLDGFWQSEGYFASNAATIRRELTFTRQPGPMYARY 
                   
               
               
                   
                   
                   
                   lutea ] 
                   
                   
                 RQQIEQTQTPVSVHIRRGDYVSHPEFSQSFGALDDTYYQTALAQINGQFPDATLLVFSDDPEWVRQHMRF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 ERPHVLVENTGPDADVDDLQLMSLCHHHIIANSSFSWWGAWLNPRPDKRVIAPKQWFRNKPWNTADLI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PAGWVRL 
                   
               
               
                   
               
               
                   Bacteroidetes ;  
                 WP_008618094.1 
                 495893515 
                 glycosyl 
                 24.15 
                   
                 MRLIKMTGGLGNQMFIYAMYLKMKTIFPDVRIDLSDMVHYQVHYGYEMNKVFHLPRTEFCINRSLKKIIEF 
                 196 
               
               
                   Capnocytophaga   
                   
                   
                 transferase 
                   
                   
                 LLFKTILERKQGGSLVPYTRKYHWPWIYFKGFYQSEKYFAGIEKEVREAFVFDIRRASRRSLRAMQEIKADPH 
                   
               
               
                 sp. oral taxon  
                   
                   
                 [ Bacteroidetes ] 
                   
                   
                 AVSIHVRRGDYLLEKHWKALGCICQSSYYLNALAELEKRVKHPHYYVFSEDLNWVRQNLPLIKAEFIDWNKG 
                   
               
               
                 329 str. F0087;  
                   
                   
                   
                   
                   
                 EDSWQDMMLMSHCRHHIICNSTFSWWGAWLNPLPDKIVIAPERWTQTTDSADVVPESWLKVSIG 
                   
               
               
                 
                   Paraprevotella 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   clara  YIT 11840 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Smaragdicoccus 
                 
                 WP_018159152.1 
                 516906936 
                 protein 
                 24.08 
                   
                 MADVVVTLAGGLGNQLFQTAYAKNLEARGHRVTLDGTVVRWTRGLHIDPQICGLKILNATPPAPVPGRLA 
                 197 
               
               
                 
                   niigatensis 
                 
                   
                   
                 [ Smaragdicoccus   
                   
                   
                 ATVLRRALATRLRFGPDGRIVRTQRTLEFDEQYLNLNSPGRYRVEGYWQCERYFSDVGQTVRKVFLDMLGR 
                   
               
               
                   
                   
                   
                   niigatensis ] 
                   
                   
                 HVSYNGLSRLPAMADPSSISLHVRRGDYVTANFIDPLALEYYERALEELAVPSPRIFVFSDDLDWATRELGR 
                   
               
               
                   
                   
                   
                   
                   
                   
                 ICDVIPVEPDWTSHPGGEIFLMSQCSHHIIANSSFSWWGAWLDGRTSSRVVAPRQWFSLETYSARDIVPDR 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WTKV 
                   
               
               
                   
               
               
                   Bacteroides   
                 WP_022012576.1 
                 547279005 
                 family 11 
                 24.05 
                   
                 MIHLILGGGLGNQMFQYAFARSLALQYNENISFNTILYKELKNEERSFSLGHLNINTMCIVETPDENKRIWEL 
                 198 
               
               
                 
                   fragilis 
                 
                   
                   
                 glycosyl- 
                   
                   
                 FNKQIFHQKIARKILPASIRWWWMSNRNIYANVCGPYKYYHPRHRSQNTTIIHGGFQSWKYFKEHQSMIK 
                   
               
               
                 CAG: 558 
                   
                   
                 transferase 
                   
                   
                 AELKVITPISEPNKKILKEIQNSNSICVHIRRGDFLSAQFSPHLEVCNKDYYEKAIKMISSQIENPTFFIFSNT 
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                 HEDLVWIRKNYNIPQNSVYVDLNNPDYEELRLMYNCKHFILSNSSFSWWAQYLSESKNKIIIAPKIWDKRKGID 
                   
               
               
                   
                   
                   
                   fragilis   
                   
                   
                 FSDIYMPEWIIIK 
                   
               
               
                   
                   
                   
                 CAG: 558] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Desulfovibrio 
                 
                 WP_022657592.1 
                 550904402 
                 protein 
                 24.05 
                   
                 MSFSIDVAAIQRMALVKVDGGLGSQMWQYALSLAVGKSSSFTVKHDLSWFRHYAKDIRGIENRFFILNSVF 
                 199 
               
               
                 
                   desulfuricans 
                 
                   
                   
                 [ Desulfovibrio   
                   
                   
                 TNINLRLASENERLFFHIALNRYPDSICNFDPDILALKQPTYLGGYYVNAQYVTSAEKEIREAYVFAPAVEES 
                   
               
               
                   
                   
                   
                   desulfuricans ] 
                   
                   
                 NQAMLQTIHAAPMPVAVHVRRGDYIGSMHEVLTPRYFERAFKILAAALQPKPTFFVFSNGMEWTKKAFAGL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PYDFVYVDANDNDNVAGDLFLMTQCKHFTISNSSLSWWGAWLSQRAENKTVIMPSKWRGGKSPIPGEC 
                   
               
               
                   
                   
                   
                   
                   
                   
                 MRVEGWHMCPVE 
                   
               
               
                   
               
               
                 
                   Hoeflea 
                 
                 WP_007199917.1 
                 494373839 
                 alpha-1,2- 
                 24.05 
                   
                 MHGGLGNQLFQYAVGRAVALRTGSELLLDTREFTSSNPFQYDLGHFSIQAKVANSSELPPGKNRPLAYAW 
                 200 
               
               
                   phototrophica ;  
                   
                   
                 fucosyl- 
                   
                   
                 WRKFGRSPRFVREQDLGYNARIETIEADCYLHGYFQSQKYFEDIASILWKDLSFRQAISGENASMAERIQSA 
                   
               
               
                 
                   Hoeflea 
                 
                   
                   
                 transferase, 
                   
                   
                 PSVSMHIRRGDYLTSAKARSTHGAPDLGYYGRALGEIRARSGSDPVVYLFSDDPDWVRNNMRMDANLVT 
                   
               
               
                 
                   phototrophica 
                 
                   
                   
                 [ Hoeflea   
                   
                   
                 VAINDGKTAFEDLRLMSLCDHNIIVNSTFSWWGAWLNPSLDKIVVAPKRWFADPKLSNPDITPPGWLRLGD 
                   
               
               
                 DFL-43 
                   
                   
                   phototrophica ] 
                   
                   
                   
                   
               
               
                   
               
               
                   Vibrio   cholerae ; 
                 WP_002030616.1 
                 487957217 
                 glycosyl 
                 24.04 
                   
                 MKIISFSGGLGNQLFQYAFYLYLKDNSDFGNIFLDFSFYESQNKRDAVIRNFYGVDSLDIIKQSSYVRGKFLI 
                 201 
               
               
                   Vibrio   cholerae   
                   
                   
                 transferase, 
                   
                   
                 LKLINKFRFFNNLLEFVDKENGLDETLLSTNKVFFDGYWQSYRYVKDYKSNIKELFSFYDFKGNILEVRKKIC 
                   
               
               
                 O1 str. 87395 
                   
                   
                 family 11  
                   
                   
                 QSNSVCMHVRRGDYVAEKNTKLVHGVCSLQYYRDALNNIKNVDNSIDHIFIFSDDIDWVKNNISFDIPVTVVD 
                   
               
               
                   
                   
                   
                 [ Vibrio   
                   
                   
                 FVGQSVPDYAEMLLFSCGKHKVIANSTFSWWGAFLSDRNGVIVSPKKWFAKEEKNYDEIFIEGSLRL 
                   
               
               
                   
                   
                   
                   cholerae ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_022784718.1 
                 551041074 
                 protein 
                 24.03 
                   
                 MIIVRFRGGMGNQMFQYAFLRYLEMKGATLKADLSEFKCMKTHAGYELDKAFDLHPAEASYKEIRAVADYI 
                 202 
               
               
                   bacterium   
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 PVMHRFPFSRKVFEILYKKETKRVEAEGPKKSHISEEKYFDMSEDERLHLASSSEDLYMDGFWIKPDMYDDE 
                   
               
               
                 NK4A179 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 VLKCFTFSKTLDEKYKGTIEDEHSCSVHVRCGDYTGTGLDILGKEYYEKAAEKILSEDADVKFYVFSDDREKA 
                   
               
               
                   
                   
                   
                 NK4A179] 
                   
                   
                 EKLLSPFMKKMVFCDTPASHAYDDMYLMSRCRHHIIANSTFSFWGARLSADKSGITICPKYEDKNNTANRLV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 HEGWQML 
                   
               
               
                   
               
               
                 
                   Cecembia 
                 
                 WP_009185692.1 
                 496476931 
                 Glycosyl 
                 24.01 
                   
                 MIIMKFMGGLGNQIYQYALGRKLSELHNSFLASDIHIYKNDPDREFVLDKFNIKVKHLPWKVIKLLNSDYALK 
                 203 
               
               
                   lonarensis ;  
                   
                   
                 transferase 
                   
                   
                 FDKVFHTEFYHELVLEKALESKDIPRKNNLYLRGSWGNRKYYEDYIDKISDEITLKEKFKTKDFNTVNKKVKNS 
                   
               
               
                 
                   Cecembia 
                 
                   
                   
                 family 11 
                   
                   
                 DSVGIHIRRGDYEKVAHFKNFYGLLPPSYYSAAVDFIGNRIEKSNFFIFSDDTDWVKENLPFLKDSFFVSDIIG 
                   
               
               
                   lonarensis   
                   
                   
                 [ Cecembia   
                   
                   
                 SVDYLEFELLKNCKHQIIANSTFSWWAARLNSNPAKIVIKPKRWFADDRQQAVYEIEDSYYIKEAIKL 
                   
               
               
                 LW9 
                   
                   
                   lonarensis ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_004295547.1 
                 490423336 
                 protein 
                 24 
                   
                 MKIVNILGGLGNQMFVYAMYLALKEAHPEEEILLCRRSYKGYPLHNGYELERIFGVEAPEAALSQLARVAYP 
                 204 
               
               
                   ovatus ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 FFNYKSWQLMRHFLPLRKSMASGTTQIPFDYSEVTRNDNVYYDGYWQNEKNFLSIRDKVIKAFTFPEFRDE 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   ovatus ] 
                   
                   
                 KNKALSDKLKSVKTASCHIRRGDYLKDPIYGVCNSDYYTRAITELNQSVNPDMYCIFSDDIGWCKENFKFLIG 
                   
               
               
                 ovatus  
                   
                   
                   
                   
                   
                 DKEVVFVDWNKGQESFYDMQLMSLCHYNIIANSSFSWWGAWLNNNDDKVVVAPERWMNKTLENDPI 
                   
               
               
                 ATCC 8483 
                   
                   
                   
                   
                   
                 CDNWKRIKVE 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_022125287.1 
                 547668508 
                 glycosyl- 
                 23.99 
                   
                 MRLIKMTGGLGNQMFIYAFYLKMKKLFPHTKIDLSDMMHYHVHHGYEMNRVFALPHTEFCINRTLKKLM 
                 205 
               
               
                   coprocola   
                   
                   
                 transferase 
                   
                   
                 EFLLCKVVYERKQKNGSMEAFEKKYAWPLIYFKGFYQSERFFADIEDDVRKTFCFNMELINSRSREMMKIID 
                   
               
               
                 CAG: 162 
                   
                   
                 family 11 
                   
                   
                 ADEHAVSIHIRRGDYLLPKFWANAGCVCQLPYYKNAITELEKHESTPSFYVFSDDIEWVKQNLSLPNAHYID 
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                 WNQGNDSWQDMMLMSHCRNHIICNSTFSWWGAWLNPRKNKTVIVPSRWFMKEETPYIYPVSWIKVPIN 
                   
               
               
                   
                   
                   
                 
                   coprocola 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 CAG: 162] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_007835585.1 
                 495110765 
                 glycosyl 
                 23.99 
                   
                 MRLIKVTGGLGNQMFIYAFYLRMKKYYPKVRIDLSDMMHYKVHYGYEMHRVFKLPHTEFCINQPLKKIIEFL 
                 206 
               
               
                   dorei ;  
                   
                   
                 transferase 
                   
                   
                 FFKKIYERKQAPNSLRAFEKKYFWPLLYFKGFYQSERFFADIKDEVREAFTFDRSKANSRSLDMLDILDKDEN 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 [ Bacteroides   
                   
                   
                 AVSLHIRRGDYLQPKHWATTGSVCQLPYYQNAIAEMSKRVTSPSYYIFSDDIVWVRENLPLQNAVYIDWNT 
                   
               
               
                   dorei  DSM 
                   
                   
                   dorei ] 
                   
                   
                 GEDSWQDMMLMSHCKHHIICNSTFSWWGAWLNPSIDKTVIVPSRWFQYSETPDIYPTGWIKVPVD 
                   
               
               
                 17855;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   dorei   
                   
                   
                   
                   
                   
                   
                   
               
               
                 CL03T12C01 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides ;  
                 WP_007662951.1 
                 494936920 
                 protein 
                 23.97 
                   
                 MIIVRLWGGLGNQLFQYSFGQYLEIETDKKVFYDVASFGTSDQLRKLELCSFIPDIPLYNAYFTRYTGVKNRL 
                 207 
               
               
                 
                   Bacteroides 
                 
                   
                   
                 [ Bacteroides ] 
                   
                   
                 FKALFQWSNTYLSESMFDICLLEKARGKIFLQGYWQEEKYATYFPMQKVLSEWKNPNVLSEIEENIRSAKISV 
                   
               
               
                   intestinalis   
                   
                   
                   
                   
                   
                 SLHVRRGDYFSPKNINVYGVCTEKYYEQAIDRANSEIEEDKQFFVFSDDILWVKNHVSLPESTVFVPNHEISQ 
                   
               
               
                 DSM 17393; 
                   
                   
                   
                   
                   
                 FAYIYLMSLCKVNIISNSTFSWWGAYLNQHKNQLVIAPSRWTFTSNKTLALDSWTKI 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   intestinalis   
                   
                   
                   
                   
                   
                   
                   
               
               
                 CAG: 564 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_016283022.1 
                 511028838 
                 protein 
                 23.95 
                   
                 MIVIHVMGGLGNQLYQYALYEKLRALGREVKLDVYAYRQAEGAEREWRALELEWLEGIRYEVCTAAERQQ 
                 208 
               
               
                   bacterium  A4 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 LLDNSMRLADRVRRRLTGRRDKTVRECAAYMPEIFEMDDVYLYGFWGCEKYYEDIIPLLQEKIVFPESSNPK 
                   
               
               
                   
                   
                   
                   bacterium  A4] 
                   
                   
                 NADVLRAMAGENAVSVHIRRKDYLTVADGKRYMGICTDAYYKGAFRYITERVERPVFYIFSDDPAFAKTQF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 CEENMHVVDWNTGRESLQDMALMSRCRHNICANSTFSIWGARLNRHPDKIMIRPLHHDNYEALDARTV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 HEYWKGWVLIDADGKV 
                   
               
               
                   
               
               
                 
                   Phaeobacter 
                 
                 YP_006574665.1 
                 399994425 
                 protein 
                 23.91 
                   
                 MIITRLHGRLGNQMFQYAAGRALADRLGVSVALDSRGAELRGEGVLTRVFDLDLATPDILPPLRQRAPLGY 
                 209 
               
               
                   gallaeciensis ;  
                   
                   
                 PGA1_c33070 
                   
                   
                 ALWRGLGQHLGTGPKLRREVGLGYNPDFVDWSDNSYLHGYWQSERYFAQSAERIRRDFTFPEYSNQQNA 
                   
               
               
                 
                   Phaeobacter 
                 
                   
                   
                 [ Phaeobacter   
                   
                   
                 EMAARIGETNAISLHVRRGDYLTLAAHVLCDQAYYEAALAQVLDGLEGQPTVYVFSDDPQWAKENLPLPC 
                   
               
               
                 
                   gallaeciensis 
                 
                   
                   
                 
                   gallaeciensis 
                 
                   
                   
                 DKVVVDFNGADTDYEDMRLMSLCKHNIIGNSSFSWWAAWLNQTPDRRVAGPTKWFGDPKLNNPDILPP 
                   
               
               
                 DSM 17395 = CIP 
                   
                   
                 DSM 17395 = 
                   
                   
                 DWLRISV 
                   
               
               
                 105210 
                   
                   
                 CIP 105210] 
                   
                   
                   
                   
               
               
                   
               
               
                   Firmicutes   
                 WP_021849028.1 
                 546362318 
                 protein 
                 23.88 
                   
                 MSGGLGNQMFQYALYLKLRSLGREVCFDDKSQYDEETFRNSSQKRRPKHLDIFGITYPSAGKEELEKLTDGA 
                 210 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Firmicutes   
                   
                   
                 MDLPSRIRRKILGRKSLEKNDRDFMFDPSFLEETEGYFCGGFQSPRYFAGAEEEVRKAFTFPEELLCPKEGCS 
                   
               
               
                 CAG: 791 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 RQEQKMLEQSASYAERIRKANCEAADRGVPGGGSASIHLRFGDYVDKGDIYGGICTDAYYDTAIRCLKERD 
                   
               
               
                   
                   
                   
                 CAG: 791] 
                   
                   
                 PGMIFFVFSNDEEKAGEWIRYQAERSENLGRGHFVLVKGCDEDHGYLDLYLMTLCRNHVIANSSFSWWAS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 FMCDAPDKMVFAPSIWNNQKDGSELARTDIYADFMQRISPRGTRLSDRPLISVIVTAYNVAPYIGRALDSV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 CGQTWKNLEIIAVDDGSSDETGAILDRYAAGDSRIQVVHTENRGVSAARNEGIAHARGEYIGFVDGDDRA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 HPAMYEAMIRGILSSGADMAVVRYREVSAEETLTDAEEQVASFDPVLRASVLLQQRDAVQCFIRAGMAEE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 EGKIVLRSAVWNKLFHRRLLRDNRFPEGTSAEDIPFTTRALCLSKKVLCVPEILYDYVVNRQESIMNTGRAER 
                   
               
               
                   
                   
                   
                   
                   
                   
                 TLTQEIPAWRTHLELLKESGLSDLAEESEYWFYRRMLSYEEEYRRCSETAKEAKELQERILKHRDRILELAEE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 HSFGRRGDRERLKLYVNSPRQYFLLSDLYEKTVVNWKNRPDKT 
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 YP_003829733.1 
                 302669773 
                 glycosyl 
                 23.84 
                   
                 MRKRIIALNGGLGNQMFQYAFARMLEDRKHCLIEFDTGFYSTVNDRKLAIQNYNIHKYDFCNHEYYNKIRLL 
                 211 
               
               
                   proteoclasticus ; 
                   
                   
                 transferase 11 
                   
                   
                 FQKIPFVAWLAGTYKEYSEYQLDPRVFLFNYRFYYGYWQNKQYFENISNDIRNELSYIGNVSEKENALLNML 
                   
               
               
                 
                   Butyrivibrio 
                 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 EAHNAIAIHVRRGDYTQEGYNKIYISLSKEYYKRAVSIACKELGDNNIPLYVFSDDIDWCKANLADIGNVTFV 
                   
               
               
                   proteoclasticus   
                   
                   
                 
                   proteoclasticus 
                 
                   
                   
                 DNTISSSADIDMLMMKKSRCLITANSTFSWWSAWLSDRDDKIVLVPDKWLQDEEKNTKLMKAFICDKWKI 
                   
               
               
                 B316 
                   
                   
                 B316] 
                   
                   
                 VPV 
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_008768245.1 
                 496043738 
                 protein 
                 23.81 
                   
                 &gt;gi|496043738|ref|WP_008768245.1|protein[ Bacteroides  sp. 
                 212 
               
               
                 2_1_16 
                   
                   
                 [ Bacteroides   
                   
                   
                 2_1_16]MQVVARIIGGLGNQMFIYATARALALRIDADLILDTQSGYKNDLFKRNFLLDSFCISYRKANCFQK 
                   
               
               
                   
                   
                   
                 sp. 2_1_16] 
                   
                   
                 YDYYLGEKVKSLGKKTHFSVIPFMKYISENTSCDFVDGLLKKHILSVYLDGYWQNEAYFKDYASIIKKDFQFCQ 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VNDLRTLSEAEIIKKSITPVAIGVRRYQELNSHQNTKVTDLDFYQKAINYIESKVDNPTFFIFSEDQEWVKNNL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 EQKSNFIMISPKEGNYSALNDMYLISLCKHHIVSNSSFYWWGAWLANNKNKIVVASDCFLNPQSIPDSWIKF 
                   
               
               
                   
               
               
                 
                   Desulfomicrobium 
                 
                 YP_003159045.1 
                 256830317 
                 glycosyl 
                 23.76 
                   
                 &gt;gi|256830317|ref|YP_003159045.1|glycosyl transferase family protein  
                 213 
               
               
                   baculatum ;  
                   
                   
                 transferase 
                   
                   
                 [ Desulfomicrobium   baculatum  DSM 
                   
               
               
                 
                   Desulfomicrobium 
                 
                   
                   
                 family protein 
                   
                   
                 4028]MAKIVTRIMGGIGNQLFCYAAARRLALVNHAELVIDDVTGFSRDRVYRRRYMLDHFNISARKATNYE 
                   
               
               
                 
                   baculatum 
                 
                   
                   
                 [ Desulfomicrobium   
                   
                   
                 RMEPFERYRRGLAKYISKKLPFFEREYIEQERIEFDPRFLEYRTYNNIYIDGLWQSENYFKDVEDIIRDDLKII 
                   
               
               
                 DSM 4028 
                   
                   
                 
                   baculatum 
                 
                   
                   
                 PPTDLENINIAKKIKNIQNTIAMHVRWFDLPGINLGNNVSTYYYHRAIAMMEQRINAPHYFLFSDNLEAVHSKL 
                   
               
               
                   
                   
                   
                 DSM 4028] 
                   
                   
                 DLPEGRVTFVSNNDGDDNAYADLWLMSQCKHFITANSTFSWWGAWLGESRDSVVLVPRFSPDGGVTS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WCFTGLIPERWEQVSSIR 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_021584236.1 
                 545304945 
                 galactoside  
                 23.76 
                   
                 MDIVLIFNGLGNQMSQYAFYLAKRQRNNHTVYCVFGPRTQYSLDKLFDIPYRHNAVLVLLYRALDKAHFSN 
                 214 
               
               
                   pleuritidis ;  
                   
                   
                 2-alpha-L- 
                   
                   
                 HRWLRRLLRPTLQLLGVKMIVEPLSRDFDMRHFTHQKGIVFYRGGWHSELNFTAVADAVKRRFRFPEIQD 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                 fucosyl- 
                   
                   
                 AAVLAVIDRIKSCQSVSLHLRRGDYLGLSEFQGVCTEAYYEHAIAYFESQIESPEYFVFSDDPTYAREQFGAD 
                   
               
               
                   pleuritidis   
                   
                   
                 transferase 
                   
                   
                 PNFHIIDLNHGEDAWCDLLMMTQCRYNIIANSTFSWWGAWLNDNPSKIVVHPRYHLNGVETRDFYPRNW 
                   
               
               
                 F0068 
                   
                   
                 [ Prevotella   
                   
                   
                 ICIE 
                   
               
               
                   
                   
                   
                   pleuritidis ] 
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_008763191.1 
                 496038684 
                 glycosyl 
                 23.75 
                   
                 MKVIWFNGNLGNQVFYCKYKEFLHNKYPNETIKYYSNSRSPKICVEQYFRLSLPDRIDSFKVRFVFEFLGKFFR 
                 215 
               
               
                 1_1_14 
                   
                   
                 transferase, 
                   
                   
                 RIPLKFVPKWYCFRKSLNYEASYFEHYLQDKSFFEKEDSSWLKAKKPDNFSEKYLIFENLICNTNSVAVHIRRG 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 DYIKPGSDYEDLSATDYYEQAIKKATEVYLDSQFFFFSDDLEFVKNNFKGDNIYYVDCNRGADSYLDILLMSQ 
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                 AKINIIANSTFSYWGAYMNHEKKKVMYSDLWFRNESGRQMPNIMLDSWICIETKRK 
                   
               
               
                   
                   
                   
                 sp. 1_1_14] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Agromyces 
                 
                 WP_022893737.1 
                 551273588 
                 protein 
                 23.65 
                   
                 MVGRVGIARRQAADVSCTDGEGLVAWRIRTGEIVLGLQGGIGNQLFEWAFAMALRSIGRRVLFDAVRCR 
                 216 
               
               
                 
                   subbeticus 
                 
                   
                   
                 [ Agromyces   
                   
                   
                 GDRPLMIGPLLPASDWLAAPVGLALAGATKAGLLSDRSWPRLVRQRRSGYDPSVLERLGGTSYLLGTFQSA 
                   
               
               
                   
                   
                   
                   subbeticus ] 
                   
                   
                 RYFDGVEHEVRAAVRALLEGMLTPSGRRFADELRADPHRVAVHVRRGDYVSDPNAAVRHGVLGAGYYDQ 
                   
               
               
                   
                   
                   
                   
                   
                   
                 ALEHAAALGHVRRVWFSDDLDWVREHLARDDDLLCPADATRHDGGEIALIASCATRIIANSSFSWWGGW 
                   
               
               
                   
                   
                   
                   
                   
                   
                 LGAPSSPAHPVIAPSTWFADGHSDAAELVPRDWVRL 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_007133870.1 
                 494220705 
                 alpha-1,2- 
                 23.59 
                   
                 MIATTLFGGLGNQMFIYATAKALSLHYRTPMAFNLRQGFEQDYKYQRHLELNHFKCQLPTAKWITFNYKG 
                 217 
               
               
                   salivae ;  
                   
                   
                 fucosyl- 
                   
                   
                 ELNIKRISRRIGRNLLCPHYQFIKEKEPFHYEKRLFEFTNKNIFLEGYWQSPRYFENYSDEIRRDFQLKSILP 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                 transferase 
                   
                   
                 HTITDELQMLKGTGKPLVMLGIRRYQEVKDKKDSPYPLCNKDYYAKAISHVQEQLPAPLFVVFTQEQAWAMNN 
                   
               
               
                 
                   salivae 
                 
                   
                   
                 [ Prevotella   
                   
                   
                 LPTNANLYFVKEKDNAWATIADMYLMTQCQHAIISNSTFYWWGAWLQHPIENHIVVAPNNFINRDCVCD 
                   
               
               
                 DSM 15606 
                   
                   
                   salivae ] 
                   
                   
                 NWIILD 
                   
               
               
                   
               
               
                   Carnobacterium   
                 YP_008718687.1 
                 554649641 
                 glycosyl 
                 23.57 
                   
                 MIFVDLSEGLGNQMFQYAYSRYLQELYGGTLYLNTSSFKRKNSTRSYSLNNFYLYENVKLPSKFRRVIYNFYS 
                 218 
               
               
                 sp. WN1359 
                   
                   
                 transferase 
                   
                   
                 KTIRMFIKKVIRMNPYSDKYYFSMIPYGFYVSSQVFKYLTVPTTKRHNIFVMGTWQTNKYFQSINDKIKDELK 
                   
               
               
                   
                   
                   
                 [ Carnobacterium   
                   
                   
                 VKTEPNELNKKLITEINSNQSVCVHIRLGDYTNPEFDYLHVCTSDYYLKGMDYIVSKVKEPNFYIFSNSSSDIE 
                   
               
               
                   
                   
                   
                 sp. WN1359] 
                   
                   
                 WIKNNYNFKYKVKYIDLNNPDFEDFRLMYNCKHFIISNSTFSWWAQFLSNNDKKIIVAPSKWQKSNENEAK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DIYLDHWKLIEIE 
                   
               
               
                   
               
               
                   Butyrivibrio  sp. 
                 WP_022762290.1 
                 551018062 
                 glycosyl 
                 23.55 
                   
                 MLIIQIAGGLGNQMQQYAMYRKLLKAGADRNIKLDTKWFDEDKQSGVLAKRKLELEYFTGLPLPVCSESER 
                 219 
               
               
                 AD3002 
                   
                   
                 transferase 
                   
                   
                 ARFTDRSVARKVVEKLVPGMGSRFTESCMYHPEIFELKDKYIEGYFACQKYYDDIMGELQELFVFPTHPDEEI 
                   
               
               
                   
                   
                   
                 [ Butyrivibrio   
                   
                   
                 NIKNMNLMNEMEMVPSVSVHIRRGDYLDPENAALFGNIATDAYYDSAMEYFKAIDPDTHFYIFTNDPEYA 
                   
               
               
                   
                   
                   
                 sp. AD3002] 
                   
                   
                 REKYADPGRYTIVDHNTGKYSLLDIQLMSHCRGNICANSTFSFWGARLNRRKDKIPVRTLVMRNNQPVTPE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 LMHEYWPGWVLVDKDGKVR 
                   
               
               
                   
               
               
                   Clostridium  sp.  
                 WP_021636935.1 
                 545396682 
                 glycosyl- 
                 23.55 
                   
                 MIVIRVMGGLGNQMQQYALYEKFKALGKETRLDTSWFDNASMQENVLARRSLELRFFDNLTYEACTPQE 
                 220 
               
               
                 KLE 1755 
                   
                   
                 transferase, 
                   
                   
                 REALLGKEGFFNKLERKLFPSKNKHFYESEMFHPEIFKLDNVYLEGHWACEKYYHDIMPLLQSKIIFPKTDNI 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 QNNMLKNKMNSENSVSIHIRRGDYLDPENAAMFGGICTDSYYKSAEGYIRNRVTNPHFYLFSDDPAYLREHY 
                   
               
               
                   
                   
                   
                 [ Clostridium   
                   
                   
                 KGEEYTVVDWNHGADSFYDMELMSCCKHNVCANSTFSFWGARLNRTEKKIVIRPAKHKNSQQAEPERM 
                   
               
               
                   
                   
                   
                 sp. KLE 1755] 
                   
                   
                 HELWENWVIIDEEGRIV 
                   
               
               
                   
               
               
                   Bacteroides;   
                 YP_001300694.1 
                 150005950 
                 glycosyl 
                 23.47 
                   
                 MKFFVFGGGLGNQLFQYSYYRYLKKKYPSERILGIYPDSLKAHNGIEIDKWFDIELPPTSYLYNKLGILLYRV 
                 221 
               
               
                 
                   Bacteroides 
                 
                   
                   
                 transferase 
                   
                   
                 NRFLYNHGYRLLFCNRVYPQSMKHFFQWGDWQDYSIIKQINIFEFRSELPIGKENMEFLKKMETCNSISVHIR 
                   
               
               
                   vulgatus   
                   
                   
                 family protein 
                   
                   
                 RGDYLKTDLIHIYGGICTSKYYREAIKFMEQEVEEPFFFFFSDDCLYVETEFADIRNKIIISHNRDDRSFFDM 
                   
               
               
                 ATCC 8482; 
                   
                   
                 [ Bacteroides   
                   
                   
                 YLMAHAKNMILANSTFSCWAAYLNRTAKIIITPDRWVNTDFSKLEALPNEWIKIRV 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   vulgatus  ATCC 
                   
                   
                   
                   
               
               
                   dorei  DSM 
                   
                   
                 8482] 
                   
                   
                   
                   
               
               
                 17855;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   massiliensis   
                   
                   
                   
                   
                   
                   
                   
               
               
                 dnLKV3 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Paraprevotella 
                 
                 WP_008626629.1 
                 495902050 
                 glycosyl 
                 23.47 
                   
                 MRLIKMTGGLGNQMFIYAMYLKMRAVFPDTRIDLSDMVHYRVHYGYEMNKVFNLPRTEFRINRSLKKIIEF 
                 222 
               
               
                   xylaniphila ;  
                   
                   
                 transferase 
                   
                   
                 LLFKTILERKQGGSLVPYIRKYHWPWIYFKGFYQSEEYFAGVEKEVREAFVFDVRRVNRKSLCAMQEIMADP 
                   
               
               
                 
                   Paraprevotella 
                 
                   
                   
                 [ Paraprevotella   
                   
                   
                 DAVSIHVRRGDYLQGKHWKSLGCICQRSYYLNALSELEKRIVHPHYYVFSEDLDWVRQYLPLENAVFIDWN 
                   
               
               
                 
                   xylaniphila 
                 
                   
                   
                   xylaniphila ] 
                   
                   
                 KGEDSWQDMMLMSHCRHHIICNSTFSWWGAWLNPSPDKIVIAPERWTQTTNSADVVPESWLKVSIG 
                   
               
               
                 YIT 11841 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Thauera  sp. 28 
                 WP_002930798.1 
                 489020296 
                 glycosyl 
                 23.47 
                   
                 MTDRALIAIVKGGLGNQLFIYAAARAMALRTGRQLYLDAVRGYLADDYGRSFRLNRFPIEAELMPEQWRV 
                 223 
               
               
                   
                   
                   
                 transferase 
                   
                   
                 ASTLRHPRAKLVRALNKYLPEAWRFYVAERGDTRPGALWNHGRNVKRVTLMGYWQDEAYFLDYAELLRR 
                   
               
               
                   
                   
                   
                 family protein 
                   
                   
                 ELGPPMPDAPEVRARGERFAGTESVFLHVRRCRYSPLLDAGYYQKAVDLACAELNKPVFMIFGDDIEWVV 
                   
               
               
                   
                   
                   
                 [ Thauera  sp. 28] 
                   
                   
                 NNIDFRGAGYERQDYDESDELADLWLMTRCRHAIIANSSFSWWAAWLGGAAGSGRHVWAPGQSGLAL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KCAKSWEAVDAQPE 
                   
               
               
                   
               
               
                 
                   Subdoligranulum 
                 
                 WP_007048308.1 
                 494107522 
                 alpha-1,2- 
                 23.44 
                   
                 MIYAELAGGLGNQMFIYAFARALGLRCGEAVTLLDRQDWRDGAPAHTACALEGLNLVPEVKILAEPGFAK 
                 224 
               
               
                   variabile ;  
                   
                   
                 fucosyl- 
                   
                   
                 RHLPRQNTAKALMIKYEQRQGLMARDWHDWERRCAPVLNLLGLHFATDGYTPVRRGPARDFLAWGYF 
                   
               
               
                   Subdoligranulum    
                   
                   
                 transferase 
                   
                   
                 QSEAYFADFAPTIRAELRAKQAPAGVWAEKIRAAACPVALHLRRGDYCRPENEILQVCSPAYYARAAAAAA 
                   
               
               
                 
                   variabile 
                 
                   
                   
                 [ Subdoligranulum   
                   
                   
                 AAYPEATLFVFSDDIDWAKEHLDTAGLPAVWMPRGDAVGDLNLMALCRGFILSNSTYSWWAQYLAGEG 
                   
               
               
                 DSM 15176 
                   
                   
                   variabile ] 
                   
                   
                 RTVWAPDRWFAHTKQTALYQPGWHLIETR 
                   
               
               
                   
               
               
                   Firmicutes   
                 WP_021916223.1 
                 547127527 
                 protein 
                 23.4 
                   
                 MIIVEVMGGLGNQMQQYALYRKLESLGKDARLDVSWFLDKERQTKVLASRKLELSWFENLPAKYCTQEEK 
                 225 
               
               
                 
                   bacterium 
                 
                   
                   
                 [ Firmicutes   
                   
                   
                 QAILGKNNLIGKLKKKLLGGSNRHFTESDMYHPEIFDLEDAYLSGFWACEAYYADILPMLRSQIHFPDPEKGE 
                   
               
               
                 CAG: 24 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 GWDLEAAAKNKETMERMKQETSVSIHIRRGDYLDAKNAEMFGGICTDAYYEAAISYIKEQTPDAHFYVFSD 
                   
               
               
                   
                   
                   
                 CAG: 24] 
                   
                   
                 DSAYVKNAYPGKEFTVVDWNTGKNSLFDMQLMSCCNHNICANSTFSFWGARLNPSPDKVMIRPSKHKN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 SQNIVPEEMKRLWDGWVLIDGKGRII 
                   
               
               
                   
               
               
                   Prevotella  sp. 
                 WP_022310139.1 
                 547906803 
                 glycosyl 
                 23.39 
                   
                 MIITKLNGGLGNQLFEYACARNLQLKYNDVLYLDIEGFKRSPRHYSLEKFKLSSDVRMLPEKDSKSLILLQA 
                 226 
               
               
                 CAG: 474 
                   
                   
                 transferase 
                   
                   
                 ISKLNRNLAFKLGPLFGTYIWKSSNYRPLKIKNTRGKKLYLYGYWQSYEYFKENEAIIKQELNVKTEIPIECS 
                   
               
               
                   
                   
                   
                 family 11 
                   
                   
                 ELLKEINKPHSICVHVRRGDYVSCGFLHCDEAYYNRGINHIFDKHPDSNVVVFSDDIKWVKANMNFDHPVAYV 
                   
               
               
                   
                   
                   
                 [ Prevotella   
                   
                   
                 EVDVPDYETLRLMYMCKHFVMSNSSFSWWASYLSDNKEKIVVAPSYWLPANKDNKSMYLDNWTIL 
                   
               
               
                   
                   
                   
                 sp. CAG: 474] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Roseburia 
                 
                 WP_006855899.1 
                 493910390 
                 glycosyl 
                 23.38 
                   
                   intestinalis ]MRGNRGMIAVKIGDGMGNQLFNYACGYAQARRDGDSLVLDISECDNSTLRDFELDKFHL 
                 227 
               
               
                   intestinalis;   
                   
                   
                 transferase 
                   
                   
                 KYDKKESFPNRNLGQKIYKNLRRALKYHVIKEREVYHNRDHRYDVNDIDPRVYKKKGLRNKYLYGYWQHLAY 
                   
               
               
                 
                   Roseburia 
                 
                   
                   
                 family 11 
                   
                   
                 FEDYLDEITAMMTPAYEQSETVKKLQEEFKKTPTCAVHVRGGDIMGPAGAYFKHAMERMEQEKPGVRYIVF 
                   
               
               
                 intestinalis  
                   
                   
                 [ Roseburia   
                   
                   
                 TNDMERAEEALAPVLESQKKDAVGQAENRLEFVSEMGEFSDVDEFFLMAACQNQILSNSTFSTWAAYLN 
                   
               
               
                 L1-82 
                   
                   
                   intestinalis ] 
                   
                   
                 QNPDKTVIMPDDLLSERMRQKNWIILK 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_004296622.1 
                 490424433 
                 protein 
                 23.29 
                   
                 MKIVLFTPGLGNQMFQYLFYLYLRDNYPNQNIYGYYNRNILNKHNGLEVDKVFDIQLPPHTVISDASAFFIR 
                 228 
               
               
                   ovatus;   
                   
                   
                 [ Bacteroides   
                   
                   
                 ALGGLGLKYFIGKDQLSPWKVYFDGYWQNKEYFQNNVDKMRFREGFLNKKNDDILSLIRNTNSVSVHVRR 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   ovatus ] 
                   
                   
                 GDYCDSCRKDLFLQSCTPQYYESAISVMKEKFQKPVFFVFSDDIPWVKVNLNIPNAYYIDWNKKENSYLDM 
                   
               
               
                   ovatus   
                   
                   
                   
                   
                   
                 YLMSLCTASIIANSTFSFWGAMLGNKKELVIKPKKWIGDEIPEIFPPSWLSL 
                   
               
               
                 ATCC 8483 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Butyrivibrio  sp. 
                 WP_022779599.1 
                 551035785 
                 glycosyl 
                 23.25 
                   
                 MLIIQIAGGLGNQMQQYALYRKLLKYHPDGVRLDLSWFDSEVQKNMLAKREFELALFKGLPYIECKPEERA 
                 229 
               
               
                 AE3009 
                   
                   
                 transferase 
                   
                   
                 AFLDRNAAQKLSGKVLKKLGLRDNANPNVFEESRMFHPEIFELDNKYIIGYFACQKYYDDIMGDLCNLFEFP 
                   
               
               
                   
                   
                   
                 [ Butyrivibrio   
                   
                   
                 EHLDPELEKKNLELISKMEKENSVSVHIRRGDYLDPENFKILGNIATDEYYESAMKYFEDRYEKVHFYIFTS 
                   
               
               
                   
                   
                   
                 sp. AE3009] 
                   
                   
                 DHEYAREHFADESKYTIVDWNTGKDSLQDVRLMNHCLGNICANSTFSFWGARLNQRQDKVMIRTYKMRNN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QPVDPDTMHDYWKGWILIDETGREV 
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 YP_003829712.1 
                 302669752 
                 glycosyl 
                 23.23 
                   
                 MTKNEKKLIVKFQGGLGNQLYEYAFCEWLRQQYSDYEVLADLSYYKIRSAHGELGIWNIFPNINIEVASNW 
                 230 
               
               
                   proteoclasticus ; 
                   
                   
                 transferase 11 
                   
                   
                 DIIKYSDQIPIMYGGKGADRLNSVRTNVNDRFFSKRKHSYYTEISNTDVSEVINALNNGIRYFDGYWQNIDYF 
                   
               
               
                 
                   Butyrivibrio 
                 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 KGNIEDLRNKLKFSEKCDKYITDEMLRDNAVSLHVRRGDYVGSEYEKEVGLSYYKKAVEYVLDRVDQAKFFIF 
                   
               
               
                   proteoclasticus   
                   
                   
                 
                   proteoclasticus 
                 
                   
                   
                 SDDKYYAETAFEWIDNKTVVAGYDNELAHVDMLLMSRMKNNIIANSTFSLWAAYLNDSMNPLIVYPDVES 
                   
               
               
                 B316 
                   
                   
                 B316] 
                   
                   
                 LDKKTFSDWNGIK 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_018362656.1 
                 517173838 
                 protein 
                 23.23 
                   
                 MDSQFLKHIKLSGGFGNQLFQYFFGEYLKEKYNCSISFFSEPALDINQLQIHRFFPALRISHNTELRPYHYSFT 
                 231 
               
               
                 
                   nanceiensis 
                 
                   
                   
                 [ Prevotella   
                   
                   
                 QQLAYRCMRKLLLLFPFLNRKVKIENGSNYQNQSFNDTYCFDGYWQSYRYLSAFTPSLQFEDQLINDISADY 
                   
               
               
                   
                   
                   
                   nanceiensis ] 
                   
                   
                 INAIEQSEAVFLHIRRGDYLNKENQKVFAECPLNYFENAANRIKEDIKNVHFFVFSNDIQWVKSHLKLNDNE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VTFIQNEGNSCDLKDFYLMTRCKHAIISNSTFSWWAAYLINNSDKKVIAPKHWYNDISMNNATKDLIPPTW 
                   
               
               
                   
                   
                   
                   
                   
                   
                 IRL 
                   
               
               
                   
               
               
                   Ruegeria  sp. R11 
                 WP_008562971.1 
                 495838392 
                 alpha-1,2- 
                 23.23 
                   
                 MIITRLHGRLGNQMFQYAAGRALADRAGVPLALDSRGAILRGEGVLTRVFDLELADPVHLPPLKQTNPLRY 
                 232 
               
               
                   
                   
                   
                 fucosyl- 
                   
                   
                 AIWRGIGQKVGAKPYFRRERGLGYNPAFEDWGDNSYLHGYWQSQKYFQNSAERIRSDFTFPAFSNQQNA 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 EMAARIAESTAISLHVRRGDYLTFAAHVLCDQAYYDAALAKVLDGLQGDPIVYVFSDDPQWAKDNLSLPCE 
                   
               
               
                   
                   
                   
                 [ Ruegeria  sp. 
                   
                   
                 KVVVDFNGPETDFEDMRLMSLCQHNIIGNSSFSWWAAWLNQTPGRRVAGPAKWFGDPKLSNPDIFPHD 
                   
               
               
                   
                   
                   
                 R11] 
                   
                   
                 WLRISV 
                   
               
               
                   
               
               
                 
                   Winogradskyella 
                 
                 WP_020895733.1 
                 527072096 
                 alpha-1,2- 
                 23.21 
                   
                 MGNQLYEYATAKAMAVALNKKLVIDPRPILKEAPQRHYDLGLFNIQDEDFGSPFVQWLVRWVASVRLGKF 
                 233 
               
               
                   psychrotolerans   
                   
                   
                 fucosyl- 
                   
                   
                 FKTIMPFAWSYQMIRDKEEGFDESLLQQKSRNIVIEGYWQSFKYFESIRPTLLKELSFKDKPNAINQKYLDE 
                   
               
               
                 RS-3;  
                   
                   
                 transferase 
                   
                   
                 IESVNAVAVHIRRGDYVANPVANAVHGLCDMDYYKKAIAIIKDKVENPYFFIFTDDPDWAEDNFKISEHQKI 
                   
               
               
                 
                   Winogradskyella 
                 
                   
                   
                 [ Winograd - 
                   
                   
                 IKHNIGKQDHEDFRLLTNCKYFIIANSSFSWWGAWLSDYKNKIVISPNKWFNVDAVPITERIPESWIRV 
                   
               
               
                 
                   psychrotolerans 
                 
                   
                   
                 
                   skyella 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 
                   psychro- 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                   tolerans ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_022785342.1 
                 551041720 
                 protein 
                 23.2 
                   
                 MITVRIDGGFGNQMFQYAFFLHLKKTITDNKISVDLNCYNPHGSGDIFTRFKLAPEQAAPSEIKRFHRNSIYH 
                 234 
               
               
                   bacterium   
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 LLRPLDSAGITTNPYYREEDIDDLNSVLNKKRVYLRGYWQDKRYPFSVKDQLIDCFDLGKMDMTGASAENN 
                   
               
               
                 NK4A179 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 VILEQIASEESRSVGVHLRGGDYIGDPVYSGICTPEYYEAAFKHVSEKIKDPVFHIFTNDISMIEKCGLSGKYD 
                   
               
               
                   
                   
                   
                 NK4A179] 
                   
                   
                 LKITDINDEAHGWADLKLMSACRHHIISNSSFSWWAAFLGEATTEASADVINVIPEYMRQGVSAETLRCPC 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WTTVTSDGRVYPS 
                   
               
               
                   
               
               
                   Prevotella  sp.  
                 WP_009230832.1 
                 496522549 
                 alpha-1,2- 
                 23.13 
                   
                 MKIVCIKGGLGNQLFEYCRYRSLHRHDNRGVYLHYDRRRTKQHGGVWLDKAFHITLPNEPLRVKLLVMVLK 
                 235 
               
               
                 oral taxon 
                   
                   
                 fucosyl- 
                   
                   
                 TLRRLHLFKRLYREEDPRAVLIDDYSQHKQYITNAAEILNFRPFEQLDYAEEIQTTPFAVSVHVRRGDYLLLA 
                   
               
               
                 317 str. 
                   
                   
                 transferase 
                   
                   
                 NKSNFGVCSVHYYLSAAVAVRERHPESRFFVFSDDMEWAKENLNLPNCVFVEHAQAQPDHADLYLMSLCK 
                   
               
               
                 F0108;  
                   
                   
                 [ Prevotella   
                   
                   
                 GHIIANSTFSFWGAYLSKGSSAIAIYPKQWFAEPTWNVPDIFPAHWMAL 
                   
               
               
                   Prevotella   
                   
                   
                 sp. oral 
                   
                   
                   
                   
               
               
                 sp. oral  
                   
                   
                 taxon 317] 
                   
                   
                   
                   
               
               
                 taxon 317 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Butyrivibrio  sp. 
                 WP_022765796.1 
                 551021633 
                 glycosyl 
                 23.1 
                   
                 MLIIQIAGGLGNQMQQYAVYTKLRGMGKDVRLDLSWFDPSVQKNMLAPREFELSMFEGVDYTECTAEER 
                 236 
               
               
                 XPD2006 
                   
                   
                 transferase 
                   
                   
                 DSFLKQGMIANVTGKMLKKLGLRDEANPKVFSEKEMYHPEIFELEDRYIKGYFACQKYYDDIMGELWEKYT 
                   
               
               
                   
                   
                   
                 [ Butyrivibrio   
                   
                   
                 FPAHSDPDLHTRNMALVERMEKETSVSVHIRRGDYLDPSNVEILGNIATEEYYQGAMDYFSVKDPDTHFYI 
                   
               
               
                   
                   
                   
                 sp. XPD2006] 
                   
                   
                 FTSDHEYAREKFSDESKYTIVDWNSGRNSVQDLMLMSHCKGNICANSTFSFWGARLNRRPDKTVIRTYKM 
                   
               
               
                   
                   
                   
                   
                   
                   
                 RNNQPVNPDIMHDYWKGWILMDEKGSII 
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 WP_022752717.1 
                 551008140 
                 protein 
                 23.08 
                   
                 MIIIKLQGGLGNQLFLYGLYKNLKHLKRDVKMDIESGFEEDKLRVPCLKSMGLDYEVATRDEIVAIRDSYMDI 
                 237 
               
               
                 
                   fibrisolvens 
                 
                   
                   
                 [ Butyrivibrio   
                   
                   
                 FSRIRRKITGRKTFDYYEPEDGNFDPRVLEQTHAYLDGYFQSEKYFGDSDDRKKLKDELLKEKIRVLDSSDTL 
                   
               
               
                   
                   
                   
                   fibrisolvens ] 
                   
                   
                 KDLYNMMSSGSSVSLHIRRGDYLTPGIMETYGGICTDEYYDIAMNRIKNEYPDSKFFIFSNDIDWCKEKYGSR 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DDVIFVDSCDEHEGLTNVSGDQDDIQVQGDIKEHGNNSLRDAAELYLMSACKHHILANSSFSWWGAWLS 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DHEGMTIAPSKWLNNKNMTDIYTKDMLLI 
                   
               
               
                   
               
               
                   Cylindro - 
                 WP_006278973.1 
                 493321658 
                 Glycosyl 
                 23.05 
                   
                 MKKTVVLLKGGLGNQMFQYAFARSISLKNSSKLVIDNWSGFTFDYKYHRQYELGTFSIVGRPANLTEKFPF 
                 238 
               
               
                 
                   spermopsis 
                 
                   
                   
                 transferase 
                   
                   
                 WFYELKSKFFPRLPKVFQQQFYGLLINEVGGEYIPEIEETKISQNCWLNGYWQSPLYFQKHSDSITRELMPPE 
                   
               
               
                   raciborskii ;  
                   
                   
                 family 11 
                   
                   
                 PMEKHFLELGKLLRETESVALGIRLYEESKNPGSHSSSGELKSHFEINQAILKLRELCNGAKFFVFCTHRSPL 
                   
               
               
                   Cylindro - 
                   
                   
                 [ Cylindro - 
                   
                   
                 LQELALPENTIFVTHDDGYVGSMERMWLLTQCKHHIFTNSTFYWWGAWLSQKFYIQGSQIVFAADNFINSDA 
                   
               
               
                 
                   spermopsis 
                 
                   
                   
                 
                   spermopsis 
                 
                   
                   
                 IPKHWKPF 
                   
               
               
                 
                   raciborskii 
                 
                   
                   
                   raciborskii ] 
                   
                   
                   
                   
               
               
                 CS-505 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_007368154.1 
                 494609908 
                 alpha-1,2- 
                 23.05 
                   
                 MKIVNFQGGLGNQMFIYAFSRYLSRLYPQEKIYGSYWSRSLYVHSAFQLDRIFSLQLPPHNLFTDCISKLAR 
                 239 
               
               
                   multiformis ;  
                   
                   
                 fucosyl- 
                   
                   
                 FFERLRLVPVEETPGSMFYNGYWLDKKYWEGIDLSEMFCFRNPDLSAEAGAVLSMIERSNAVSVHIRRGDYQ 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                 transferase 
                   
                   
                 SEEHIEKFGRFCPPDYYRIATERIRQREDDPLFFVFSDDMMWVKSNMDVPNAVYVDCHHGDDSWKDMF 
                   
               
               
                 
                   multiformis 
                 
                   
                   
                 [ Prevotella   
                   
                   
                 LMAKCRHNIIANSTFSFWAAMLNANPDKVVVYPQRWFCWPSPDIFPEMWLPVTEKEIKSSF 
                   
               
               
                 DSM 16608 
                   
                   
                   multiformis ] 
                   
                   
                   
                   
               
               
                   
               
               
                 Bacteroides sp. 
                 WP_022384635.1 
                 548151455 
                 protein 
                 23 
                   
                 MIIVNMACGLANRMFQYAFYLSLKERGYNVKVDFYKSATLPHENVPWNDIFPYAEIDQVSNFRVLILGGGA 
                 240 
               
               
                 CAG: 462 
                   
                   
                 [ Bacteroides   
                   
                   
                 NLLSKLRRKYLPSLTNVITMSTAFDTDLQIDDDRKDKYIIGVFQSAAMVEGVCKKVKQCFSFLPFTDLRHLQL 
                   
               
               
                   
                   
                   
                 sp. CAG: 462] 
                   
                   
                 EKEMQECESVAIHVRKGNDYQQRIWYQNTCFMDYYRKAIAEIKGKVKDPRFYVFTDNADWVRRNFTDFD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 YKMVEGNPVYGWGSHFDMQLMSRCKYNIISNSTYSWWGAYLNANRNKIVICPNIWFNPESCNEYTSCKL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 LCKGWIAL 
                   
               
               
                   
               
               
                 
                   Desulfovibrio 
                 
                 WP_005984176.1 
                 492830222 
                 Glycosyl 
                 23 
                   
                 MRIGILYICTGKYTVFWNHFFTSCEQHFLREHEKHYYIFTDGEIAHLNCNRVHRIEQQHLGWPDSTLKRFHM 
                 241 
               
               
                   africanus;   
                   
                   
                 transferase 
                   
                   
                 FERIADTLRQNSDFIVFFNANMVFLRDVGKEFLPTREQALVFHRHPGLFRRPAWLLPYERRPESTAYIPYGS 
                   
               
               
                 
                   Desulfovibrio 
                 
                   
                   
                 family 11/ 
                   
                   
                 GSIYVCGGVNGGYTQPYLDFVAMLRRNIDIDVERGIIARWHDESHINRFVIGRHYKIGHPGYVYPDRRNLPF 
                   
               
               
                   africanus  PCS 
                   
                   
                 Glycosyl- 
                   
                   
                 PRIIRVIDKASVGGHTFLRGQTPEPAPEEQSKTVAKKLRSQLKRPCMPRAAQDEPIILARMMGGLGNQMFI 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 YAAARVLAERQGAQLHLDTGKLSGDSIRQYDLPAFSIDAPLWHIPCGCDRIVQAWFALRHVAAGCGMPKP 
                   
               
               
                   
                   
                   
                 family 6 
                   
                   
                 TMQVLRSGFHLDQRFFSIRHSAYLIGYWQSPHYWRGHEDRVRSSFDLTRFERPHLREALAAVSQPNTISVH 
                   
               
               
                   
                   
                   
                 [ Desulfovibrio   
                   
                   
                 LRRGDFRAPKNSDKHLLIDGSYYERARKLLLEMTPQSHFYIFSDEPEEAQRLFAHWENTSFQPRRSQEEDLLL 
                   
               
               
                   
                   
                   
                   africanus ] 
                   
                   
                 MSRCSASIIANSSFSWWGAWLGRPKQHVIAPRMWFTRDVLMHTYTLDLFPEKWILL 
                   
               
               
                   
               
               
                   Roseburia  sp. 
                 WP_022518697.1 
                 548374190 
                 protein 
                 22.98 
                   
                 MILIHVMGGLGNQLYQYALYEKMKSLGKKVKLDTYAYNDAAGEDKEWRSLELDRFPAIEYDKATSEDRTKL 
                 242 
               
               
                 CAG: 100 
                   
                   
                 [ Roseburia  sp. 
                   
                   
                 LDNSGLLTAKIRRKLLGRKDKTIRESKEYMPEIFHMDDVYLYGFWNCERYYEDIIPLLQDKLQFPISNNPRNQ 
                   
               
               
                   
                   
                   
                 CAG: 100] 
                   
                   
                 QCIEQMQKENAVSIHIRRTDYLTVADGARYMGICTEDYYKGAMAYIEERVSNPVYYIFSDDVEYAKQHYHQ 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DNMHVVDWNSKADSIYDMQLMSKCKHNICANSTFSMWAARLNQNKEKIMIRPLHHDNYETTTATQVK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QNWKNWILLDQNGQVCE 
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_022742385.1 
                 550997676 
                 protein 
                 22.96 
                   
                 MTMNIIRMSGGLGSQMFQYALYLKLKSMGKEVKFDDINEYRGEKARPIMLAVFGIEYPRATWDEITSFTDG 
                 243 
               
               
                   bacterium  10-1 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 SMDLLKRLRRKIFGRKAIEYEEQGFYDPNVLNFDSMYLRGNFQSEKYFQDIKEEVRKLYRFSTLEDMRLPERL 
                   
               
               
                   
                   
                   
                   bacterium  10-1] 
                   
                   
                 YKATKACLDGIESSESVGLHMYRSDSRVDGELYDGICFGNYYKGAVRFIQDKVPDAKFYIFSNEPKWVRGW 
                   
               
               
                   
                   
                   
                   
                   
                   
                 VVDLIQSQIQEGMSPSQVKEMEKRFVMVEANTEYTGYLDMMLMSKCKHNIISNSSFSWWSAWMNDHP 
                   
               
               
                   
                   
                   
                   
                   
                   
                 EKVVVAPDRWSSDKEGNEIYTTGMTLVNEKGRVNYTIHENSTVK 
                   
               
               
                   
               
               
                 
                   Prevotella 
                 
                 WP_004362670.1 
                 490496500 
                 protein 
                 22.96 
                   
                 MILSYITGRLGNQLFEYAYARSLLLKRGKNEELILNFSLVRAAGKEIEGFDDNLRYFNVYSYTELDKDIVLS 
                 244 
               
               
                   nigrescens ;  
                   
                   
                 [ Prevotella   
                   
                   
                 KGDLLQLFIYILFKLDQKLFRIIKKEKWFSFFRRFGIIFQDYLDNISNLIIPRTKNVFCYGKYENPKYFDDI 
                   
               
               
                 
                   Prevotella 
                 
                   
                   
                   nigrescens ] 
                   
                   
                 RSILLKEFTPRIPPLKNNDQLYSVIESTNSVCISIRRGDFLCDKFKDRFLVCDKEYFLEAMEEAKKRISNST 
                   
               
               
                 
                   nigrescens 
                 
                   
                   
                   
                   
                   
                 FIFFSDDIEWVRENIHSDVPCYYESGKDPVWEKLRLMYSCKHFIISNSTFSWWAQYLSRNEEKVVIAPDRWS 
                   
               
               
                 F0103 
                   
                   
                   
                   
                   
                 NVPGEKSFLLSNSFIKIPIGILP 
                   
               
               
                   
               
               
                   Bacteroides  sp. 
                 WP_022353235.1 
                 547952493 
                 fucosyl 
                 22.95 
                   
                 MIYVEINGRLGNNMFEIAAAKSLTDEVTLWCKGDWQLNCIKMYSDTLFKNYPIVKSLPNNIRIYEEPEFTFH 
                 245 
               
               
                 CAG: 875 
                   
                   
                 transferase 
                   
                   
                 PIPYKENQDLLIKGYFQSYKYLDREKVLKLYPCPMPVKLDIEKRFGDILSQYTVVSINVRRGDYLNLPHRHPFV 
                   
               
               
                   
                   
                   
                 [ Bacteroides   
                   
                   
                 GKKFLERAMLWFGDKVHYIISSDDIEWCKAHFKQFDNVHYLTNSYPLLDLYIQTACHHNIISNSSFSWWGA 
                   
               
               
                   
                   
                   
                 sp. CAG: 875] 
                   
                   
                 YLNNHPQKIVIAPHRWFGMSTNINTQDLLPPEWMIEQCVYEPKVFLKALPLHAKYLLKRVLK 
                   
               
               
                   
               
               
                   Prevotella  sp. 
                 YP_008444280.1 
                 532354444 
                 protein 
                 22.9 
                   
                 MDSQLLKHIKLSGGFGNQLFQYFFGEYLKEKYNCSISFFSEPALDINQLQIHRFFPTLRISHNTELRRFHYAFT 
                 246 
               
               
                 oral taxon 299  
                   
                   
                 HMPREF0669_00176 
                   
                   
                 QQLAYRCMRKLLLLFPFLNRKVKIENGSNYQNQSFNDTYCFDGYWQSYRYLSAFTPSLQFEDQLINDISADY 
                   
               
               
                 str. F0039;  
                   
                   
                 [ Prevotella   
                   
                   
                 INAIEQSEAVFLHIRRGDYLNKENQKVFAECPLNYFENAVNKIKEGNKTYHFFVFSNDIEWVKCHLKLNNNE 
                   
               
               
                   Prevotella   
                   
                   
                 sp. oral taxon 
                   
                   
                 VTFIQNEGSSCDLKDFYLMTRCKHAIISNSTFSWWAAYLINNNDKKVIAPKRWYNDLSMNNATKDLIPPTW 
                   
               
               
                 sp. oral 
                   
                   
                 299 str. F0039] 
                   
                   
                 IRL 
                   
               
               
                 taxon 299 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Paraprevotella 
                 
                 WP_008628783.1 
                 495904204 
                 alpha-1,2- 
                 22.87 
                   
                 MKIVCLKGGLGNQMFEYCRFRDLMDSGNGKVYLFYDRRRLKQHDGLRLSDCFELELPSCPWGIRLVVWGL 
                 247 
               
               
                   xylaniphila ;  
                   
                   
                 fucosyl- 
                   
                   
                 KICRAIGVLKRLYDDEKPDAVLIDDYSQHRRFIPNARRYFSFRQFLAELQSGFVQMIRAVDYPVSVHVRRGD 
                   
               
               
                 
                   Paraprevotella 
                 
                   
                   
                 transferase 
                   
                   
                 YLHPSNSSFVLCGVDYFRQAIAYVRKKRPDARFFFFSDDMEWVRENLWMEDAVYVEHTELMPDYMDLYL 
                   
               
               
                 
                   xylaniphila 
                 
                   
                   
                 [ Paraprevotella   
                   
                   
                 MTLCRGHIISNSTFSFWGAYLAVDGNGMKIYPRRWFRDPTWITPPIFSEEWVGL 
                   
               
               
                 YIT 11841 
                   
                   
                   xylaniphila ] 
                   
                   
                   
                   
               
               
                   
               
               
                   Dethio - 
                 WP_005658864.1 
                 491897177 
                 glycosyl 
                 22.84 
                   
                 MFQYAFGRALALDLGLDLKLDISNFGSDSRPFSLGIYSLTKNIPFGCYLSTSTRLKVKMTKKLRRWGVWGMD 
                 248 
               
               
                 
                   sulfovibrio 
                 
                   
                   
                 transferase 
                   
                   
                 KNMPGVLVEPFPPVLVSLDEVLSEKLSHLFVDGYWQSEKYFSRYSDVIRSDFRVIEESSAFLAWKKRMLSEP 
                   
               
               
                   peptidovorans ; 
                   
                   
                 family 11 
                   
                   
                 GGSISVHVRRGDYVTDSSANRVHGVLPIEYYLRAKEILNTISDGLVFYVFTDDPVWARNNLCLGDKTIYVSGE 
                   
               
               
                   Dethio - 
                   
                   
                 [ Dethio - 
                   
                   
                 DLKDYEELALMSCCDHHVVANSSFSWWGAWLGQDTSTVTIAPGRWFRKMDSSFVIPDNWIKIWT 
                   
               
               
                 
                   sulfovibrio 
                 
                   
                   
                 
                   sulfovibrio 
                 
                   
                   
                   
                   
               
               
                   peptidovorans   
                   
                   
                   peptidovorans ] 
                   
                   
                   
                   
               
               
                 DSM 11002 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_016229292.1 
                 510896192 
                 protein 
                 22.83 
                   
                 MIIIQVMGGLGNQLQQYALYRKFVRMGKEARLDISWFLDKEKRGEVLAERELELDYFDRLIYETCTPEEKEQ 
                 249 
               
               
                   bacterium  10-1 
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 LIGSEGVAGKLKRKFLPGRIRWFHESKIYHPELLQMENMYLSGYFACEKYYADILYDLREKIQFPVNDHPKNI 
                   
               
               
                   
                   
                   
                   bacterium  10-1] 
                   
                   
                 KMAQEMQERESVSVHLRRGDYLDEKNTAMFGNICTDAYYCKAIEYMKTLCSKPHFYIFSDDIPYVRQRFTG 
                   
               
               
                   
                   
                   
                   
                   
                   
                 EEYTVVDINHGRDSFFDMWLMSRCRHNICANSTFSFWGARLNSNDNKIMIRPTIHKNSQVFVKEEMEQL 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WPGWKFISPDGGIK 
                   
               
               
                   
               
               
                 
                   Treponema 
                 
                 WP_016525279.1 
                 513872223 
                 protein 
                 22.82 
                   
                 MFCAAFVEALKHAGQKVFVDTSLYNKGTVRSGIDFCHNGLETEHLFGIKFDEADKADVHRLSTSAEGLLNRI 
                 250 
               
               
                   maltophilum ;  
                   
                   
                 [ Treponema   
                   
                   
                 RRKYFTKKTHYIDTVFRYTPEVLSDKSDRYLEGFWQTEKYFLPIESDIRTLFRFRQPLSEKSAAVQSALQAQ 
                   
               
               
                 
                   Treponema 
                 
                   
                   
                   maltophilum ] 
                   
                   
                 EPASLSASIHVRRGDFLHTKTLNVCTETYYNNAIEYAAKKYAVSAFYVFSDDIQWCREHLNFFGARSVFIDW 
                   
               
               
                 
                   maltophilum 
                 
                   
                   
                   
                   
                   
                 NIGADSWQDMVLMSMCRCNIIANSSFSWWAAWLNAASDKIVLAPAIWNRRQLEYADRYYGYDYSDVIPET 
                   
               
               
                 ATCC 51939 
                   
                   
                   
                   
                   
                 WIRIPI 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_016276676.1 
                 511022363 
                 protein 
                 22.79 
                   
                 MKLVSFTAGLGNQLFQYCFYRYLLNKFPNEKIYGYYNKKWLKKHGGIIIEHFFDVKLPRSTRWINLYGQYLRI 
                 251 
               
               
                   massiliensis ;  
                   
                   
                 [ Bacteroides   
                   
                   
                 IYKCFSCGVSKDDDFEMNRTMFVGYWQDQCFFSGINISYKKNLVISEKNTWLLGEILKCNSVAIHFRRGDYM 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   massiliensis ] 
                   
                   
                 LPQFKKIFGEVCTVKYYLKSIRKVEEKISEPVFFVFSDDIDWVKQNFTFNKVYFVDWNKGQNSFWDMYLMS 
                   
               
               
                 
                   massiliensis 
                 
                   
                   
                   
                   
                   
                 QCSANIIANSTFSFWGAYLNKNNPFVIYPQKWVRTNLKQPNIFPKTWMAL 
                   
               
               
                 dnLKV3 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 Enterococcus  
                 YP_006376560.1 
                 389869137 
                 family 11 
                 22.71 
                   
                 MIVLTLGGGLGNQMFQYGYARYIQKIHREKFIYINDSEVIKEADRFNSLGNLNTVNIKVLPRIISKPLNETERL 
                 252 
               
               
                   faecium ;  
                   
                   
                 glycosyl- 
                   
                   
                 VRKIMVRLFGVAGFNESAIFQSLNKFGIYYHPSVYKFYESLKTGFPIKIIEGGFQSWKYLETCPEIKQELRVKY 
                   
               
               
                 
                   Enterococcus 
                 
                   
                   
                 transferase 
                   
                   
                 EPMGENLRLLNLISQSESVCVHIRRGDYLSPKYKHLNVCDYQYYFESMNYIISKLNNPTFFIFSNTSDDLDWIK 
                   
               
               
                 
                   faecium 
                 
                   
                   
                 [ Enterococcus   
                   
                   
                 ENYSLPGKIVYVKNDNPDYEELRLMYSCKHFIISNSTFSWWAQYLSNNSGIVIAPEIWNRLNHDGIADLYMP 
                   
               
               
                 DO; 
                   
                   
                   faecium  DO] 
                   
                   
                 NWITMKVNR 
                   
               
               
                 
                   Enterococcus 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   faecium   
                   
                   
                   
                   
                   
                   
                   
               
               
                 EnGen0035 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroides ;  
                 WP_004313284.1 
                 490442319 
                 glycosyl 
                 22.67 
                   
                 MDVVVIFNGLGNQMSQYAYYLAKKKVNPNTKVIFDIMSKHNHYGYDLERAFGIEVNKTLLIKVLQIIYVLSR 
                 253 
               
               
                   Bacteroides  sp. 
                   
                   
                 transferase 
                   
                   
                 KFRLFKSVGVRTIYEPLNYDYTPLLMQKGPWGINYYVGGWHSEKNFMNVPDEVKKAFMFREQPNEDRFN 
                   
               
               
                 2_l_22;  
                   
                   
                 family 11 
                   
                   
                 EWLQVIRGDNSSVSVHIRRGDYMNIEPTGYYQLNGVATLDYYHEAIDYIRQYVDTPHFYVFSNDLDWCKE 
                   
               
               
                   Bacteroides  sp. 
                   
                   
                 [ Bacteroides ] 
                   
                   
                 QFGVENFFYIECNQGVNSWRDMYLMSECHYHINANSTFSWWGAWLCKFEDSITVCPERFIRNVVTKDFY 
                   
               
               
                 2_2_4; 
                   
                   
                   
                   
                   
                 PERWHKIKSC 
                   
               
               
                   Bacteroides   
                   
                   
                   
                   
                   
                   
                   
               
               
                 sp. D1; 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   xylanisolvens   
                   
                   
                   
                   
                   
                   
                   
               
               
                 SD CC 2a;  
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   xylanisolvens   
                   
                   
                   
                   
                   
                   
                   
               
               
                 SD CC 1b; 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   ovatus  CAG: 22 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Synechococcus   
                 YP_004322362.1 
                 326781960 
                 glycosyl- 
                 22.6 
                   
                 MIGFNALGRMGRLANQMFQYASLKGIARNTGVDFCVPYHEEAVNDGIGNMLRTEIFDSFDLQVNVGLLN 
                 254 
               
               
                 phage 
                   
                   
                 transferase 
                   
                   
                 KGHAPVVQERFFHFDEELFRMCPDHVDIRGYFQTEKYFKHIEDEIREDFTFKDEILNPCKEMIAGVDNPLAL 
                   
               
               
                 S-SM2 
                   
                   
                 family 11 
                   
                   
                 HVRRTDYVTNSANHPPCTLEYYEAALKHFDDDRNVIVFSDDPAWCKEQELFSDDRFMISENEDNRIDLCLM 
                   
               
               
                   
                   
                   
                 [ Synechococcus   
                   
                   
                 SLCDDFIIANSTYSWWGAWLSANKDKKVIAPVQWFGTGYTKDHDTSDLIPDGWTRIATA 
                   
               
               
                   
                   
                   
                 phage S-SM2] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Geobacter 
                 
                 YP_006720295.1 
                 404496189 
                 glycosyl- 
                 22.58 
                   
                 MDIHVLSYGLGNQLSQYAFFINRRQLMQRAYAFYAFKQHNGYELDRIFGLKEGLPWYLQFVRVVFRLGISR 
                 255 
               
               
                   metallireducens ; 
                   
                   
                 transferase 
                   
                   
                 RFYSKRTADFVLSLFRIKVIDEAYNYEFDPSLLKPWFGIRILYGGWHDSRYFHPSEAAVRTAFSFPPLDDVND 
                   
               
               
                 
                   Geobacter 
                 
                   
                   
                 [ Geobacter   
                   
                   
                 AILQQIDAVYGVSIHVRRGDYLKGINSNLFGGIATLEYYRNAIGWAITYCKHRSLEIKFYVFSDDIDWCKQNL 
                   
               
               
                   metallireducens   
                   
                   
                 
                   metallireducens 
                 
                   
                   
                 GLRDAVYVSGNSKTDSWKDILLMSHCRANIIANSTFSWWAAWLNQQPNKVVICPTKFINTDSPNQTIYPA 
                   
               
               
                 GS-15;  
                   
                   
                 GS-15] 
                   
                   
                 AWHQIEG 
                   
               
               
                 
                   Geobacter 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   metallireducens 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 RCH3 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_022780989.1 
                 551037245 
                 protein 
                 22.58 
                   
                 MIIVRFHGGLGNQMFEYAFYRYMTNKYGADNVIGDMTWFDRNYSEHQGYELKKVFDIDIPAIDYKTLAKI 
                 256 
               
               
                   bacterium   
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 HEYYPRYHRFAGLRYLSRMYAKYKNKHLKPTGEYIMDFGPSQYIHNDAFDKLDTNKDYYIEGVFCSDAYIKY 
                   
               
               
                 NK4A136 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 YENQIKKDLTFKPNYSQHTKDMLPKIEETNSVAIHVRRGDYVGNVFDIVTPDYYRQAVNYIRERVENPVFFV 
                   
               
               
                   
                   
                   
                 NK4A136] 
                   
                   
                 FSDDMDYIKANFDFLGDFVPVHNCGKDSFQDMYLISRCRHMIIANSSFSYFGALLGEKDSTIVIAPKKYKADE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DLALARENWVLL 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_008144634.1 
                 495419937 
                 protein 
                 22.56 
                   
                 MGFIVNMACGLANRMFQYSYYLFLKKQGYKVTVDFYRSAKLAHEKVAWNSIFPYAEIKQASRLKVFLWGG 
                 257 
               
               
                   coprophilus ; 
                   
                   
                 [ Bacteroides   
                   
                   
                 GSDLCSKVRRRYFPSSTNVRTTTGAFDASLPANTARNEYIIGVFLNASIVEAVDDEIKKCFTFLPFTDEMNLR 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                   coprophilus ] 
                   
                   
                 LKKEIEECESVAIHVRKGKDYQSRIWYQNTCSMEYYRKAILQMKEKLQHSKFYVFTDNVDWVKENFQEIDYT 
                   
               
               
                   coprophilus  DSM 
                   
                   
                   
                   
                   
                 LVEGNPADGYGSHFDMQLMSLCKHNIISNSTYSWWSAFLNRNPEKVVIAPEIWFNPDSCDEFRSDRALCK 
                   
               
               
                 18228 = JCM  
                   
                   
                   
                   
                   
                 GWIVL 
                   
               
               
                 13818 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Bacteroidetes ;  
                 WP_008619736.1 
                 495895157 
                 alpha-1,2- 
                 22.53 
                   
                 MKIVCLKGGLGNQMFEYCRFRDLMESGHDEVYLFYDHRRLKQHNGLRLSDCFELELPSCPWGIKLVVWGL 
                 258 
               
               
                   Capnocytophaga   
                   
                   
                 fucosyl- 
                   
                   
                 KICRAVGVLKRLYDDEKPEAVLIDDYSQHRRFIPNARRYFFFRQFLAELQSGFVQMIRAVDYPVSVHVRRGD 
                   
               
               
                 sp. oral 
                   
                   
                 transferase 
                   
                   
                 YLHPSNSSFGLCGVDYFQQAIAYVRKKRPDARFFFFSDDMEWVRENLWMEDAVYVEHTELLPDYVDLYL 
                   
               
               
                 taxon 329   
                   
                   
                 [ Bacteroidetes ] 
                   
                   
                 MTLCRGHIISNSTFSFWGAYLAVDGNGMKIYPRRWFRDPTWTSPPIFSEEWVGL 
                   
               
               
                 str. F0087; 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Paraprevotella 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                   clara   
                   
                   
                   
                   
                   
                   
                   
               
               
                 YIT 11840 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                   Butyrivibrio  sp. 
                 WP_022770361.1 
                 551026242 
                 glycosyl 
                 22.47 
                   
                 MLIIQIAGGLGNQMQQYAVYTKLREMGKDVKLDLSWFDPQVQKNMLAPREFELPIFGGTDYEECSAYERD 
                 259 
               
               
                 NC2007 
                   
                   
                 transferase 
                   
                   
                 ALLKQGAFAAIAGKVLKKLGLRDEANPKVFSEKEMYHPEVFELEDKYIKGYFACQKYYGDIMDKLQEKFIFPE 
                   
               
               
                   
                   
                   
                 [ Butyrivibrio   
                   
                   
                 HSDPDLHARNMALVERMEREPSVSVHIRRGDYLDPSNVEILGNIATEQYYQGAMDYFTVKEPDTHFYIFTS 
                   
               
               
                   
                   
                   
                 sp. NC2007] 
                   
                   
                 DHEYAREKFSDESKYTIVDWNNGKNSVQDLMLMSHCKGNICANSTFSFWGARLNKRPDKTVIRTYKMRN 
                   
               
               
                   
                   
                   
                   
                   
                   
                 NQPVNPQIMHDYWKGWILMDEKGSII 
                   
               
               
                   
               
               
                 
                   Paraprevotella 
                 
                 WP_008628536.1 
                 495903957 
                 glycosyl 
                 22.45 
                   
                 MKILVFTGGLGNQMFAYAFYLYLKRLFPQERFYGLYGKKLSEHYGLEIDKWFKVSLPRQPWWVLPVTGLFY 
                 260 
               
               
                   xylaniphila ;  
                   
                   
                 transferase 
                   
                   
                 LYKQCVPNSKWLDLNQEICKNPRAIVFFPFKFTKKYIPDDNIWLEWKVDESGLSEKNRLLLSEIRSSDCCFVH 
                   
               
               
                 
                   Paraprevotella 
                 
                   
                   
                 [ Paraprevotella   
                   
                   
                 VRRGDYLSPTFKSLFEGCCTLSYYQRALKSMKEISPFVKFVCFSDDIQWVKQNLELGNRAVFVDWNSGTDS 
                   
               
               
                 
                   xylaniphila 
                 
                   
                   
                   xylaniphila ] 
                   
                   
                 PLDMYLMSQCRYGIMANSTFSYWGARLGRKKKRIYYPQKWWNHGTGLPDIFPNTWVKI 
                   
               
               
                 YIT 11841 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Blautia 
                 
                 WP_005944761.1 
                 492742598 
                 protein 
                 22.44 
                   
                 MEIHVYLTGRLGNQLFQYAFARHLQKEYGGKIICNIYELEHRSEKAAWVPGKFNYEMSNYKLNDSILIEDIKL 
                 261 
               
               
                 
                   hydrogenotrophica 
                 
                   
                   
                 [ Blautia ] 
                   
                   
                 PWFADFSNPIIRIVKKVIPRIYFNLMASKGYLLWQKNSYINIPAIRNNEIIVNGWWQDVRFFHDVEAELSNEI 
                   
               
               
                 DSM 
                   
                   
                   
                   
                   
                 VPTTKPISENEYLYNIAERENSVCVSIRGGNYLVPKVKKKLFVCDKEYFYNAIELIKSKVRNAIFIVFSDDLE 
                   
               
               
                 10507;  Blautia ;  
                   
                   
                   
                   
                   
                 WVKSYIKLEEKFPECKFYYESGKDTVEEKLRMMTKCKHFIISNSSFSWWAQYLAKNENKIVIAPDAWFTNGDK 
                   
               
               
                 
                   Blautia 
                 
                   
                   
                   
                   
                   
                 NGLYIDDWILIPTQTKDM 
                   
               
               
                 
                   hydrogenotrophica 
                 
                   
                   
                   
                   
                   
                   
                   
               
               
                 CAG: 147 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Geobacter 
                 
                 YP_001952981.1 
                 189425804 
                 glycoside 
                 22.44 
                   
                 MITVLLNGGLGNQLFQYAAGRALAEKHDVELLLDLSRLQHPKPGDTPRCFELAPFNIKASLLAEEGRQPLGS 
                 262 
               
               
                   lovleyi ;  
                   
                   
                 hydrolase  
                   
                   
                 YQACMHRLLLKASIPLWGSIILKEQGCGFDPLIFRAPSSCILDGFWQSECYFKQITSLLQQELSLKAPSPALR 
                   
               
               
                 
                   Geobacter 
                 
                   
                   
                 family 
                   
                   
                 KASSVLSDATVAVHVRRGDYVTNPAAASFHGICSQDYYQAAVANILTSYPDSQFLVFSDDPAWCQEHLDLG 
                   
               
               
                 lovleyi SZ 
                   
                   
                 protein 
                   
                   
                 QPFRLAADFGLNGSAEELVLISRCAHQIIANSSFSWWGAWLNPSPHKLVVAPCRWFTDPAITTNDLLPETW 
                   
               
               
                   
                   
                   
                 [ Geobacter   
                   
                   
                 VRLP 
                   
               
               
                   
                   
                   
                   lovleyi  SZ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Lachnospiraceae 
                 
                 WP_022781176.1 
                 551037435 
                 protein 
                 22.41 
                   
                 MVISHLSGGFGNQLYSYAFAYAVAKARKEELWIDTAIQDAPWFFRNPDILNLNIKYDKRVSYKIGEKKIDKIF 
                 263 
               
               
                   bacterium   
                   
                   
                 [ Lachnospiraceae   
                   
                   
                 NRINFRNAIGWNTKIINESDMPNIDDWFDTCVNQKGNIYIKGNWSYEKLFISVKQEIIDMFTFKNELSKEAN 
                   
               
               
                 NK4A136 
                   
                   
                 
                   bacterium 
                 
                   
                   
                 DIAQDINSQETSVGIHYRLGDYVKIGIVINPDYFISAMTSMVEKYGNPVFYSFSEDNDWVKKQFEGLPYNIKY 
                   
               
               
                   
                   
                   
                 NK4A136] 
                   
                   
                 VEYSSDDKGLEDFRLYSMCKHQIASNSSYSWWGAYLNNNPNKYIIAPTDYNGGWKSEIYPKHWDVRPFEF 
                   
               
               
                   
                   
                   
                   
                   
                   
                 LK 
                   
               
               
                   
               
               
                 
                   Bacteroides 
                 
                 WP_005840359.1 
                 492426440 
                 glycosyl 
                 22.37 
                   
                 MFHYKFLLFGGGLGNQIFEYYFYLWLRKKYPNIVFLGCYRKASFKAHNGLEISDVFDVDLPNDGGLSGRFISY 
                 264 
               
               
                   vulgatus ;  
                   
                   
                 transferase 
                   
                   
                 VLSVLSRIIPSLSMKANTEYSSKYLLINAYQPNLLFYLNEEKIKFRPFKLDEVNRRLLNSIKMESSVSIHVRR 
                   
               
               
                 
                   Bacteroides 
                 
                   
                   
                 family 11 
                   
                   
                 GDYLFGQYRDIYSNICTLAYYQKAVDKCKGILESPRFFVFSDDIEWARDVFVGREYEFVSNNIGKNSFIDMFL 
                   
               
               
                 
                   vulgatus 
                 
                   
                   
                 [ Bacteroides   
                   
                   
                 MSNCKIQIIANSTFSYWAAYLSNSLVKIYPAKWINGIERPNIFPDNWIGL 
                   
               
               
                 PC510 
                   
                   
                   vulgatus ] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Planctomyces 
                 
                 YP_004271766.1 
                 325110698 
                 glycosyl 
                 22.37 
                   
                 MIIARIENGLGNQLFKYAAGRALSLKHRTSLYTIPGSVRKPHETFILSKYFNVQAKSVSPFLLQTGFRLRLLK 
                 265 
               
               
                   brasiliensis ;  
                   
                   
                 transferase 
                   
                   
                 GYENHSFGFDPRFETTRNNTVVSGNFQSARYFLPFFDQINRELTLKPEVVDGLESVYPHVLESLRTPNSVCVH 
                   
               
               
                   Planctomyces    
                   
                   
                 family protein 
                   
                   
                 IRLGDYVSSGYDICGPEYYAKAISRLQQLHGELRAFVFSDTPQAASRFLPADIDAQIMSEFPEVRDAARSLTV 
                   
               
               
                 
                   brasiliensis 
                 
                   
                   
                 [ Planctomyces   
                   
                   
                 ERSTIRDYFLMQQCRHFVIPNSSFSYWAALLSSSDGDVIYPNRWYIDIDTSPRDLGLAPAEWTPIPLT 
                   
               
               
                 DSM 5305 
                   
                   
                   brasiliensis   
                   
                   
                   
                   
               
               
                   
                   
                   
                 DSM 5305] 
                   
                   
                   
                   
               
               
                   
               
               
                   Butyrivibrio  sp. 
                 WP_022772730.1 
                 551028648 
                 glycosyl 
                 22.36 
                   
                 MIILQIAGGLGNQMQQYALYRKLLKCGKTVKLDLSWFGPEIQKNMLAPREFELVLFKDLPFEICFKEEKDALI 
                 266 
               
               
                 AE2015 
                   
                   
                 transferase 
                   
                   
                 KQNLFQKIAGKVSQKLGKSASSNAKVFVETKMYHEEIFDLDDVYITGYFACQYYYDDVMAELQDLFVFPSHS 
                   
               
               
                   
                   
                   
                 [ Butyrivibrio   
                   
                   
                 IPELDQRNAVLASKMEKENSVSVHIRRGDYLSPENVGILGNIASDKYYESAMNYFLEKDENTHFYIFTNDHEY 
                   
               
               
                   
                   
                   
                 sp. AE2015] 
                   
                   
                 AREHYSDESRYTIIDWNTGKNSLQDLMLMSHCKGNICANSTFSFWGARLNKRPDRELVRTLKMRNNQEA 
                   
               
               
                   
                   
                   
                   
                   
                   
                 QPEIMHEYWKNWILIDENGVIV 
                   
               
               
                   
               
               
                 
                   Roseovarius 
                 
                 WP_009813856.1 
                 497499658 
                 alpha-1,2- 
                 22.34 
                   
                 MTDTPPPSQVITSRLFGGAGNQLFQYAAGRALADRLGCDLMIDARYVAGSRDRGDCFTHFAKARLRRDVA 
                 267 
               
               
                 
                   nubinhibens 
                 
                   
                   
                 fucosyl- 
                   
                   
                 LPPAKSDGPLRYALWRKFGRSPRFHRERGLGVDPEFFNLPRGTYLHGYWQSEQYFGPDTDALRRDLTLTTA 
                   
               
               
                 ISM;  
                   
                   
                 transferase, 
                   
                   
                 LDAPNAAMAAQIDAAPCPVSFHVRRGDYIAAGAYAACFPDYYRAAADHLATTLGKPLTCFIFSNDPAWAR 
                   
               
               
                 
                   Roseovarius 
                 
                   
                   
                 [ Roseovarius   
                   
                   
                 DNLDLGQDQVIVDLNDEATGHFDMALMARCAHHVIANSTFSWWGAWLNPDPDKLVVAPRNWFATQA 
                   
               
               
                 
                   nubinhibens 
                 
                   
                   
                   nubinhibens ] 
                   
                   
                 LHNPDLIPEQWHRL 
                   
               
               
                   
               
               
                 Eubacterium sp. 
                 WP_022505071.1 
                 548315094 
                 protein 
                 22.33 
                   
                 MIEVNIVGQLGNQMFEYACARQLQKKYGGEIVLNTYEMRKETPNFKLSILDYKLSENVKIISDKPLSSANAN 
                 268 
               
               
                 CAG: 581 
                   
                   
                 [ Eubacterium   
                   
                   
                 NYLVKIMRQYFPNWYFNFMAKRGTFVWKSARKYKELPELNEQLSKHIVLNGYWQCDKYFNDVVDTIREDF 
                   
               
               
                   
                   
                   
                 sp. CAG: 581] 
                   
                   
                 TPKYPLKAENEQLLEKIKSTESVCVTIRRGDFMNEKNKDTFYICDDDYFNKALSKIKELCPDCTFFGFSDDVE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 WIKKNVNFPGEVYFESGNDPVWEKLRLMSACKHFVLSNSSFSWWAQYLSDNNNKIVVAPDIWYKTGDPK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 KTALYQDGWNLIHIGD 
                   
               
               
                   
               
               
                 
                   Providencia 
                 
                 AFH02807.1 
                 383289327 
                 glycosyl- 
                 22.26 
                   
                 MKINGKESSMKIKQKKIISHLIGGLGNQLFQYATSYALAKENNAKIVIDDRLFKKYKLHGGYRLDKLNIIGE 
                 269 
               
               
                 
                   alcalifaciens 
                 
                   
                   
                 transferase 
                   
                   
                 KISSIDKLLFPLILCKLSQKENFIFKSTKKFILEKKTSSFKYLTFSDKEHTKMLIGYWQNAIYFQKYFSELK 
                   
               
               
                   
                   
                   
                 [ Providencia   
                   
                   
                 EMFVPLDISQEQLDLSIQIHAQQSVALHVRRGDYISNKNALAMHGICSIDYYKNSIQHINAKLEKPFFYIFS 
                   
               
               
                   
                   
                   
                   alcalifaciens ] 
                   
                   
                 NDKLWCEENLTPLFDGNFHIVENNSQEIDLWLISQCQHHIIANSTFSWWGAWLANSDSQIVITPDPWFNKEI 
                   
               
               
                   
                   
                   
                   
                   
                   
                 DIPSPVLSHWLKLKK 
                   
               
               
                   
               
               
                   Salmonella   
                 AFW04804.1 
                 411146173 
                 glycosyl- 
                 22.26 
                   
                 MFSCLSGGLGNQMFQYSAAYILKKNICHAQLIIDDSYFYCQPQKDTPRNFEINQFNIVFDRVTTDEEKRAISK 
                 270 
               
               
                 
                   enterica 
                 
                   
                   
                 transferase 
                   
                   
                 LRKFKKIPLPLFKSNVITEFLFGKSLLTDEDFYKVLKKNQFTVKMNACLFSLYQDSSLINKYRDLILPLFTIN 
                   
               
               
                   
                   
                   
                 [ Salmonella   
                   
                   
                 DELLQVCQQLDSYGFICEHTNTTSLHIRRGDYVTNPHAAKFHGTLSMNYYSQAMNYVDHKLGKQLFIIFSDDV 
                   
               
               
                   
                   
                   
                   enterica ] 
                   
                   
                 QWAAEKFGGRSDCYIVNNVNCQFSAIDMYLMSLCNNNIIANSTYSWWGAWLNKSEEKLVIAPRKWFAEDK 
                   
               
               
                   
                   
                   
                   
                   
                   
                 ESLLAVNDWISI 
                   
               
               
                   
               
               
                 
                   Sulfurospirillum 
                 
                 YP_003304829.1 
                 268680398 
                 protein 
                 22.18 
                   
                 MIIIKIMGGLASQLHKYSVGRALSLKYNTELKLDIFWFDNISGSDTIREYHLDKYNVVAKIATEQEIKQFKPNK 
                 271 
               
               
                   deleyianum ;  
                   
                   
                 Sdel_1779 
                   
                   
                 YLLKINNLFQKFTNWKINYRNYCNESFISLENFNLLPDNIYVEGEWSGDRYFSHIKEILQKELTLKSEYMDSTN 
                   
               
               
                 
                   Sulfurospirillum 
                 
                   
                   
                 [ Sulfuro-   
                   
                   
                 HFLAKQSSDFAHDDNASKLHCTCSLEYYKKALQYISKNLLKMKLLIFSDDLDWLKPNFNFLDNVEFEFVEGF 
                   
               
               
                 
                   deleyianum 
                 
                   
                   
                 
                   spirillum 
                 
                   
                   
                 QDYEEFHLMTLSKHNIIANSGFSLFFAWLNINHNKIIISLSEWVFEEKLNKYIIDNIKDKNILFLENLE 
                   
               
               
                 DSM 6946 
                   
                   
                 
                   deleyianum 
                 
                   
                   
                   
                   
               
               
                   
                   
                   
                 DSM 6946] 
                   
                   
                   
                   
               
               
                   
               
               
                   Pseudovibrio   
                 YP_005080114.1 
                 374329930 
                 alpha-1,2- 
                 22.15 
                   
                 MSVASQVRISGAARRRKLKPTLIVRIRGGIGNQLFQYALGRKIALETGMKLRFDRSEYDQYFNRSYCLNLFKT 
                 272 
               
               
                 sp. FO-BEG1 
                   
                   
                 fucosyl- 
                   
                   
                 QGLSATESEMSAVLWPAQSFGQTVKLCRKFYPFYQRRYIREDELLQDSETPVLKQSAYLDGYWQTWEIPFSI 
                   
               
               
                   
                   
                   
                 transferase 
                   
                   
                 MEQLRDEITLKKPMVLERLKLLQRIKSGPSAALHVRYGDYSQAHNLQNFGLCSAGYYKGAMDFLTERVPGL 
                   
               
               
                   
                   
                   
                 [ Pseudovibrio   
                   
                   
                 TFYVFSDSPERAREVVPQQENVYFSDPMQDGKDHEDLMVMSSCDHIVTANSTFSWWAAFLNGNEDKHV 
                   
               
               
                   
                   
                   
                 sp. FO-BEG1] 
                   
                   
                 IAPLKWFKNPNLDDSLIVPPHWQRL 
                   
               
               
                   
               
               
                   Prevotella  sp.  
                 WP_009236633.1 
                 496529942 
                 alpha-1,2- 
                 22.11 
                   
                 MKIVCIKGGLGNQLFEYCRYHGLLRQHNNHGVYLHYDRRRTKQHGGVWLDKAFLITLPTEPWRVKLMVM 
                 273 
               
               
                 oral taxon 472  
                   
                   
                 fucosyl- 
                   
                   
                 ALKMLRKLHLFKRLYREDDPRAVLIDDYSQHKQFITNAAEILNFRPFAQLDYVDEITSEPFAVSVHVRRGD 
                   
               
               
                 str. F0295;  
                   
                   
                 transferase 
                   
                   
                 YLLPANKANFGVCSVHYYLSAAVAVRERHPDARFFVFSDDIEWAKMNLNLPNCVFVEHAQPQPDHADLYLM 
                   
               
               
                   Prevotella   
                   
                   
                 [ Prevotella   
                   
                   
                 SLCKGHIIANSTFSFWGAYLSMGSSAIAIYPKQWFAEPTWNAPDIFLGHWIAL 
                   
               
               
                 sp. oral  
                   
                   
                 sp. oral 
                   
                   
                   
                   
               
               
                 taxon 472 
                   
                   
                 taxon 472] 
                   
                   
                   
                   
               
               
                   
               
               
                 
                   Butyrivibrio 
                 
                 WP_022752732.1 
                 551008155 
                 glycosyl 
                 22.08 
                   
                 MLIIRVAGGLGNQMQQYAMYRKLKSLGKEVKLDLSWFDVENQEGQLAPRKCELKYFDGVDFEECTDAER 
                 274 
               
               
                 
                   fibrisolvens 
                 
                   
                   
                 transferase 
                   
                   
                 AYFTKRSILTKALNKVFPATCKIFEETEMFHPEIYSFKDKYLEGYFLCNKYYDDILPFIQNEIVFPKHSDPK 
                   
               
               
                   
                   
                   
                 [ Butyrivibrio   
                   
                   
                 RMQKNEELMERMDGWHTASIHLRRGDYITEPQNEALFGNIATDAYYDAAIRYVLDKDYQTHFYIFSNDPEYA 
                   
               
               
                   
                   
                   
                   fibrisolvens ] 
                   
                   
                 REHYSDESRYTIVTGNDGDNSLLDMELMSHCRYNICANSTFSFWGARLNKRSDKEMIRTFKMRNNQEVTARE 
                   
               
               
                   
                   
                   
                   
                   
                   
                 MTDYWKDWILIDEKGNRIF 
                   
               
               
                   
               
               
                   Lewinella   
                 WP_020571066.1 
                 522059857 
                 protein 
                 22.04 
                   
                 MVISRLHSGLGNQMFQYAFARRIQLQLNVKLRIDLSILLDSRPPDGYIKREYDLDIFKLSPAYHCNPTSLRI 
                 275 
               
               
                 persica 
                   
                   
                 [ Lewinella   
                   
                   
                 LYAPGKYRWSQVVRDLARKGYPVYMEKSFSVDNTLLDSPPDNVIYQGYWQSERYFSEVANTIRKDFAFQHSI 
                   
               
               
                   
                   
                   
                   persica ] 
                   
                   
                 QPQSESLAREIRKEDSVCLNIRRKDYLASPTHNVTDETYYENCIQQMRERFSGARFFLFSDDLVWCREFFAD 
                   
               
               
                   
                   
                   
                   
                   
                   
                 FHDVVIVGHDHAGPKFGNYLQLMAQCHHYIIPNSTFAWWAAWLGERTGSVIMAPERWFGTDEFDYRDVV 
                   
               
               
                   
                   
                   
                   
                   
                   
                 PERWLKVPN 
               
               
                   
               
            
           
         
       
     
     OTHER EMBODIMENTS 
     While the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims. 
     The patent and scientific literature referred to herein establishes the knowledge that is available to those with skill in the art. All United States patents and published or unpublished United States patent applications cited herein are incorporated by reference. All published foreign patents and patent applications cited herein are hereby incorporated by reference. Genbank and NCBI submissions indicated by accession number cited herein are hereby incorporated by reference. All other published references, documents, manuscripts and scientific literature cited herein are hereby incorporated by reference. 
     While this invention has been particularly shown and described with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims.