Patent Publication Number: US-2006008803-A1

Title: Identification of tissue/cell specific marker genes and use thereof

Description:
CROSS REFERENCES TO RELATED APPLICATIONS  
      This application claims the priority of U.S. provisional patent application 60/388,994, filed Jun. 14, 2002, the disclosure of which is incorporated herein by reference in its entirety. 
    
    
     FIELD OF THE INVENTION  
      The present invention relates to a method for the identification of tissue cell specific marker genes, a method for the determination of a disease state or developmental status of cells/tissue as well as to gene expression profiling of cartilage tissue. More specifically, the invention relates to microarrays containing a plurality of selected human chondrocyte specific sequences and their use for classification of cartilage donor tissue or generation of characteristic gene expression profiles of in vitro chondrocyte cultures. Such DNA arrays find use as a standard tool of molecular biology research and clinical diagnostics for all cartilaginous or related tissues.  
     BACKGROUND OF THE INVENTION  
      Limitation on Current Microarray Technologies  
      DNA array technology, also known as biochip or microarray technology, is currently revolutionizing modern biology. In this technology, a biological sample is applied to a glass slide or chip covered with an array of immobilized DNA probes. Sample nucleic acid complementary to specific probes on the array hybridizes and can be detected with high sensitively with automated, computerized detectors. In this manner, hundreds to thousands of different individual hybridization experiments can be performed simultaneously. This allows assays of enormous complexity to be carried out—for example, an analysis of the entire gene expression profile of a cancer cell—with simplicity unimaginable only a few years ago. As a consequence many patents as well as scientific publications have accumulated during the last years. U.S. Pat. No. 6,194,158 discloses characteristic genes and gene expression useful in screening for, diagnosis of, monitoring of, and therapeutic treatment of brain cancer. U.S. Pat. No. 6,218,122 discloses methods for determining or monitoring the progression of disease states or the efficacy of therapeutic regimens within human patients. U.S. Pat. No. 6,077,673 discloses mouse arrays having a plurality of probe polynucleotides corresponding to a key mouse gene for expression analysis of critical mouse genes. A list of representative scientific papers dealing with monitoring the expression level of a large number of transcripts within a cell at any time are as follows: Schena et al., 1995, Quantitative monitoring of gene expression patterns with a complementary DNA-microarray, Science 270: 467-470; Lockhart et al., 1996, Expression monitoring by hybridization to high-density oligonucleotide arrays, Nature Biotechnology 14:1675-1680; Blanchard et al., 1996, Sequence to array: Probing the genome&#39;s secrets, Nature Biotechnology 14:1649. Qi et al., 2003, Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells PNAS 18; 100 (6):3305-10. While this list of scientific papers and patents reflects without any doubt the great potential of microarrays, there are a couple of yet unsolved problems that are more and more discussed among the scientific community. Especially, these problems are data overflow, representative sample collection, RNA processing and inappropriate data analysis. It is even suspected that within next five years, many of conclusions drawn from published data will be revised or refuted. Thus there remains a real and unmet need for advanced microarray solutions, targeted to specific tissues above all with respect to simplification and substantiation of the process of data generation and data handling. With respect to this issue the disclosed invention has made considerable contribution in the cartilage area with a cartilage-specific microarray containing a manageable number of cartilage relevant genes.  
      Limitation on the Number of Cartilage Relevant Genes  
      Until today the number of cartilage-relevant genes (genes that have been associated a potential functional role on cartilage biology, homeostasis or pathology) is very limited. Approximately, 100-200 genes have been described in the literature in any relationship to cartilage tissue. While existing publications e.g. Heller et al PNAS, 94; 2150-2155; 1997 have described analysis of inflammatory diseases of cartilage and Sekiya et al PNAS 99; 43974402; 2001 cartilage formation from stem cells with microarrays, a comprehensive analysis and determination of characteristic gene expression profiles for 2D, 3D, fetal, adult and pathological chondrocytes cell cultures cultivated under different conditions has not been performed up to now. While in patent WO01/24833 A2 a few markers have been determined that are associated with chondorcytes and their phenotype stability, it will not be possible to perform a detailed gene expression analysis and to define specific fingerprints. Therefore the possibility of characterizing culture conditions or cartilage tissue samples can not be thoroughly addressed.  
      Completion of the human genome first project draft on 2000 has revealed that the human genome comprises ˜30000-35000 human genes. Estimates show that the number and type of active genes vary significantly between different tissues and may increase up to a couple of 10000 for complex tissues, e.g. brain. As a consequence, many genes albeit fully sequenced may have yet not been, disclosed to be functionally up- or down regulated in cartilage or cartilage derived cells. The inventive approach described herein has made possible to up to now disclose a total of 467 known and additional genes being differentially expressed in a significant and objective manner within chondrocytes or chondrogenic cells.  
      By means of the already known and additionally found to be cartilage related genes, a strategy to best address and represent chondrocytes cultured under different conditions has been developed in the scope of the present invention.  
     SUMMARY OF THE INVENTION  
      In a first aspect the present invention relates to a method for the identification of tissue/cell specific marker genes comprising 
          a) taking tissue and/or cells of at least one developmental stage and/or at least one disease state, and/or     cultivating said tissue and/or cells in vitro under at least one culture condition,     b) determination of gene expression profiles of said tissue/cells and/or in vitro cultivated tissue/cells and     c) identification of specific marker genes by bioinformatic analysis of said gene expression profiles.        

      In particular, the first aspect relates to a method for the identification of tissue/cell specific marker genes comprising cultivating tissue/cells of different developmental stages and/or health conditions in vitro under different culture conditions, determination of gene expression profiles of said in vitro cultivated cartilage tissue and identification of specific marker genes by bioinformatic analysis of said gene expression profiles.  
      In a preferred embodiment said tissue is selected from the group consisting of fetal tissue, adolescent tissue, adult tissue, healthy tissue, pathological tissue, progenitor cells such as stem cells or cells derived from the same precursor lineage. Preferred culture conditions are 2D and 3D in vitro cultures and the gene expression profiles are preferably determined by means of a micro-array. The bioinformatic analysis of said gene expression profiles is preferably done by cluster software such as e.g cluster analysis.  
      In a preferred embodiment said tissue is cartilage.  
      A second aspect of the present invention relates to a method for the determination of a disease state or developmental status of cells/tissue or the physiological potential of cells/tissue. Said method comprises establishing a profile of cellular constituents, preferably a gene expression profile, of said cells or tissue, comparison of said resulting gene expression profile with gene expression profiles characteristic for a particular status or physiological potential of the examined cells or tissue.  
      Said method can e.g be used to assess the redifferentiation potential of cells or tissue, the assessment of the quality of tissue biopsies for diagnostic and prognostic purposes regarding in vitro tissue engineering applications, the assessment of the quality of in vitro produced cells such as e.g. mesenchymal cells, stem cells or embryonic cells or of in vitro produced tissue for therapeutical applications and for determining the effect of one or more growth factors, media compositions or drugs on cells or tissue. Based on said method it is e.g. possible to set up different in vitro culture conditions for cells/tissue allowing the cultivation of cells/tissue which retain their potential for differentiation.  
      In a preferred embodiment said cells or tissue is cartilage tissue or chondrocytes and the array comprises polynucleotide probes of tissue specific marker genes.  
      In a further preferred embodiment said profile is a gene expression profile which is determined by means of a micro-array.  
      A further object of the present invention is a method for the determination of characteristic profits for clinical use comprising correlating the patient data of the biopsy donor with the gene expression profile of said biopsy cells/tissue. Preferably said gene expression profile has been determined according to the above disclosed method. The resulting profiles of said method are suitable tools in the clinic allowing an evaluation of further treatments of a patient.  
      The present invention provides characteristic gene expression profiles experimentally determined by using cartilaginous tissues as from individual human donors of various ages (fetal, adolescent, adult) and health conditions (healthy and arthritic) or cells thereof cultivated under different in vitro culture conditions (2D and 3D in vitro cultures, time follow ups). From these different gene expression profiles a set of hitherto 467 markers has been deduced that can be used to design and produce a cartilage specific microarray for commercial applications in the field of R&amp;D, such as culture media development, drug screening etc., but also for clinical applications.  
      Gene expression analysis performed with such microarrays and the corresponding analytical procedure thereof can be used to assess quality control of human donor cartilage, e.g. biopsy and therefore optimization of any downstream tissue engineering process, for diagnostic evaluation of the patient and its candidate treatment methods, to ensure a cost-optimized procedure, to investigate and assess all kind of 2D- and 3D in vitro cultures performed with human chondrocytes or chondrogenic cells, e.g. stem cells, to screen all kind of drugs, e.g. hormones, growth factors within the above mentioned in vitro cultures regarding a potential beneficial effect and quality assessment of in vitro produced tissue performed by tissue engineered procedures.  
      In a further aspect the present invention provides a cartilage array comprising a plurality of different polynucleotide probe spots stably associated with a solid surface of a carrier, whereby each of said spots is made of a unique polynucleotide that corresponds to one specific cartilage marker gene.  
      A preferred cartilage array of the present invention comprises at least two spots that have different nucleotide sequences but of the same cartilage marker gene, more preferably at least 10 spots indicative for one tissue or cell status, whereby said at least 10 spots can be selected from different sequences of one gene or from different genes or a combination thereof.  
      In a preferred embodiment said polynucleotides of the array do not cross hybridize under stringent conditions with each other.  
      In a preferred embodiment of the present invention the cartilage array comprises spots that are indicative for at least two tissue or cell status, preferably 3.  
      A further preferred inventive cartilage array is an array wherein at least part of the cartilage marker genes are selected from the 467 genes listed in the description, preferably at least 10%, more preferably at least 50%, most preferably about 100%.  
      A further preferred inventive cartilage array is an array wherein at least part of the cartilage marker genes are selected from a subgroup of the 467 genes listed in the description, wherein said subgroup consists of the most tissue specific 200 genes.  
      In another preferred embodiment the status is selected from biopsies and/or 2D cultures and/or 3D cultures of healthy adult, healthy fetal/infant, undesired adult, undesired fetal/infant or progenitor cells like e.g. stem cells or cells derived from the same precursor lineage.  
      In a further preferred embodiment of the present invention the polynucleotide probes of the cartilage array have a length of at least 10 nucleotides, preferably at least 20 nucleotides. The probes can also have a length of 30 nucleotides, 50 nucleotides or 70 nucleotides. It is as well possible to use PCR derived products produced from cDNA clones.  
      In a preferred embodiment the carrier of the inventive cartilage array is attached to coated glass, nylon or any other material.  
      A further object of the present invention is a kit for use in a hybridization assay, wherein said kit comprises a cartilage array of the present invention. In a preferred embodiment said kit comprises reagents for generating a labelled target polynucleotide sample, a hybridization buffer and a wash medium. 
    
    
     DESCRIPTION OF THE FIGURES  
      The present invention will be further understood from the following description with reference to the tables and figures where:  
      Tab. I shows the determined number of all genes in the corresponding SOM analysis being differentially expressed according to microarray analyses of a variety of in vitro chondrocyte cultures according to predefined criteria. From these data sets specific expression profiles can be deduced that are characteristic for different cell culture conditions.  
      Tab. II shows the extracted and reviewed genes deduced from Tab I in order to have only single entry numbers. Since most of these genes have never been described in any relationship to cartilage, they can be considered as novel cartilage marker (positive/negative markers) or key cartilage genes.  
      Tab III shows a subset of marker genes form Tab. II that has been used for the production of a micro-array. Included is a subset from Tab II and genes known from the literature.  
      Tab IV shows the results of the analysis of the 467 cartilage specific marker genes.  
      Tab V shows the samples used in Examples 1, 2 and 3. Human chondrocytes isolated from 4 different donors were proliferated and kept in 3D-like pellet culture for 7 and 14 days resulting in a total number of 12 samples.  
       FIG. 1  shows a classical result from an analysis performed with self-organizing-maps. This software clusters all genes together in sub clusters that show a similar expression profile. The number of marker genes for the corresponding analysis e.g. 2D vs. 3D cultures (see also Tab I) corresponds to the total number of genes in the sub clusters.  
       FIG. 2  shows an example of a graphical presentation of a cluster analysis and viewed by the software treeview. This shows how cells from different origin and potential for in vitro cartilage formation are related to each other and allow a clearer classification of the cell sources. Fetal cells clearly produce different gene clusters compared to adult chondrocytes, while failures are characterized by other gene clusters. Furthermore 3D cell cultures analyzed in a time dependent manner from different donors can be distinguished among each other and gene expression profiles will be grouped accordingly.  
       FIG. 3 : SOM analysis of all culture conditions and samples described in Example 2 and in Tab V.  
       FIG. 4 : SOM analysis for proliferated chondrocytes (t0) only, for the 4 donors. Gene expression pattern corresponding to donor 2 (the second spot from left hand side in every cluster) behaves different in most clusters.  
       FIG. 5 : SOM analysis of chondrocytes kept in 3D culture condition for 7 days (t7). Gene expression pattern from donor 3 (the third spot from left hand side in every cluster) is different for example in clusters c2 and c5.  
       FIG. 6  shows self organized maps (SOM) of chondrocytes from same patients of  FIGS. 4 and 5  kept under 3D culture condition for 14 days (t14).  
       FIG. 7 : cluster analysis of all culture conditions and samples described in Example 2 and in Tab V. This figure shows a subset of 88 hierarchical clustered genes (rows) and samples (columns) demonstrating similar gene expression behavior of chondrocytes under different culture conditions. For example proliferated cells (#1, #2, #4, #5, #7, #8, #10, #11) can easily be discriminated from cells kept in 3D-like pellet culture for 14 days (3#, 6#, 9#, 12#).  
       FIG. 8 : cluster analysis of human aortic fibroblasts vs. chondrocytes. This figure shows a subset of selected clusters of human aortic fibroblasts cells compared to human chondrocytes both kept in 3D pellet cultures for 14 days. The dendrogram in the upper part of the figure shows the ability of CART-CHIP™ 300 microarray described in this invention to discriminate between different cell lines.  
       FIG. 9 : cluster analysis of Interleukin-1 treated vs. untreated human chondrocytes. This figure demonstrates a subset of representative gene clusters allowing differentiation between cells treated with Interleukin-1 from untreated cells both kept in 3D pellet cultures as well as for proliferated cells. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION  
      Definitions  
      2D cultures as used in the scope of the present invention are anchorage dependent chondrocyte cultures cultivated on plastic culture devices.  
      3D cultures as used in the scope of the present invention are chondrocytes cultured in a three dimensional environment, namely either a) scaffold-free, such as small high density pellet cultures (0.25-3.0*10 6  cells) or as high density cultures using 50*10 6  cells/ml or aliquots thereof; or b) by using a synthetic scaffold such as PGA, PLA, or mixtures thereof or biological substances such as agarose, alginate, chitosan or collagen.  
      failures as used in the scope of the present invention are chondrocytes cultured in a three dimensional environment that are not able to synthesize new extracellular matrix thereby compromising the production of new living tissue engineered cartilage equivalents.  
      gene expression profile as used in the scope of the present invention is a profile of genes that are up or down regulated according to different cell conditions.  
      fingerprint as used in the scope of the present invention refers to a gene expression profile characteristic for a cellular status.  
      tissue or cell status as used in the scope of the present invention refers to a tissue or cells therof having a certain metabolic or activity status.  
      new extracellular matrix as used in the scope of the present invention designates living cartilage-like tissue.  
      micro-array as used in the scope of the present invention is used in its original scope that encompasses embodiments today sometimes refused to as “macro-arrays”.  
      The Invention  
      The present invention provides cartilage-specific gene arrays as well as methods for their use. In the subject cartilage arrays, a plurality of polynucleotide probe spots are stably associated with the surface of a solid carrier, preferably a surface of a microscope glass slide. Each different polynucleotide probe spot is made of a unique polynucleotide that corresponds to a key cartilage gene of interest. Thus, the subject arrays find particular use in gene expression assays of key cartilage genes. In further describing the subject of the invention, the cartilage specific microarrays are first discussed, followed by a review of representative applications in which the subject arrays may be employed.  
      Arrays of the Subject Invention-General Description  
      Selection of Novel Key Cartilage Specific Genes:  
      A critical feature of the subject arrays is that all of the probe polynucleotide spots of the array correspond to human key cartilage genes that have been found through unique selection processes and criteria. As a result of said processes, up to now 467 different key human cartilage genes that are under tight transcriptional role have been discovered, some of them being not described before in any relationship to cartilage. In more detail, different microarray analyses were performed by using cartilaginous tissues as from individual human donors of various ages (fetal, adolescent, adult) and health conditions (healthy and arthritic) or cells thereof cultivated under different in vitro culture conditions (2D and 3D in vitro cultures, time follow ups). This variety of cartilage cell sources and different culture conditions was set up to grasp the highest possible number of genes differentially expressed and thus being indicative of a potential role.  
      It has been found that specific chondrocyte culture conditions are of great importance for the present invention that discloses a plurality of novel key cartilage genes as well as characteristic and meaningful gene expression patterns. For this reason, the strategy and criteria of the analysed in vitro human chondrocyte cultures are described in more detail. The principal experimental setup included both the cultivation of chondrocytes in an anchorage dependent condition, known as 2D cultures for expansion of cells e.g. where the passages is variable but at least more then one, as well as cultivation of chondrocytes in an anchorage independent condition, known as 3D cultures for (re-)differentiation and de novo tissue formation of cells. These are the key steps of any tissue engineering process where autologous tissue equivalents are produced. Since the cell source is either a small biopsy, a small bone marrow aspirate in case of mesenchymal stem cells or other tissue with a limited number of pre-chondrogenic cells, it is first necessary to isolate those cells in order to be able to multiply the cell number drastically. In case of a cartilage biopsy, cells are released from their surrounding extracellular matrix by collagenase digestion and then seeded onto the surface of plastic tissue culture flasks. The proliferation may take place either in the presence or absence of fetal serum combined with conventional DMEM/F12 medium. Cells can then be passaged by trypsin treatment over several rounds. As a major drawback of this necessary cell expansion, the cells loose their differentiated phenotype and assume a de-differentiated phenotype with altered gene expression. It is further known that with increasing number of passages the state of de-differentiation also advances. As a consequence, genes being transcriptionally upregulated under such artificial culture conditions are cartilage relevant in a manner being indicative of an undesired cellular status. It is also quite common to designate these genes as de-differentiation or negative markers. While healthy tissue in general has been found to re-differentiate in 3D culture after up to 4 passages in 2D cultures, tissue of undesired cellular status cultivated under usual conditions, such as usual culture media, usually does not re-differentiate in 3D culture after at most 4 passages in 2D culture.  
      Subculture modulated chondrocytes that do not express differentiation markers reexpress the differentiated phenotype in response to the anchorage—independence resulting from various 3D culture models, e.g. high density cultures, agarose or alginate cultures, or cultures within synthetic scaffolds such as made of polyglycolic acid (PGA), polylactic acid (PLA) or mixtures thereof. To set up three dimensional cell cultures the cells are detached after proliferation by trypsin treatment and embedded either in gel-like substances such as alginate, seeded within a porous scaffold such as PGA or cultivated as high-density cultures, only. The time for the analysis may vary and ideally addresses several time points (up to several weeks). Thus 3D in vitro chondrocyte cultures support the differentiated phenotype of chondrocytes and can be used to discover cartilage relevant genes or differentiation markers. It should be noted however, that reversibility of the de-differentiation process is dependent on the number of passages and can become irreversible or at least partially irreversible at higher passage numbers (under usual conditions at most about 4 passages). As a rule the time course of de- and re-differentiation are similar. During skeletal development, cartilage serves as a template for bone formation. Chondrocytes of fetal or infant (&lt;1 year) or growth plate cartilage pass through different stages and exhibit several distinct phenotypes, such as resting, proliferating, and hypertrophic chondrocytes. Progression through each of these phases is accompanied by profound changes in gene expression patterns. Further, evidence has accumulated that the successful sequence of cartilage repair via tissue engineering recapitulates aspects of embryonic tissue formation. For these reasons, it is important to consider fetal and infant cartilaginous tissue. Cells isolated from human fetal/infant cartilage that are cultivated in 2D and 3D culture systems as described above are especially helpful to understand the mechanisms underlying the phenotypic instability of chondrocytes and the related gene expression patterns. These 2D and 3D culture system may then be analyzed to deduce gene expression profiles and to define marker genes that are characteristic for the (re-)differentiation process. Thus maintenance of chondrocyte-specific phenotype being crucial for normal structure and biomechanical properties of articular cartilage may be better understood and have important implications for modern therapeutic biological applications.  
      The above mentioned experimental setup for 2D and 3D cultures may be even expanded to compare human adult cells with human fetal/infant chondrocytic cells of age &lt;1 year. The comparison of gene expression profiles of adult versus fetal/infant human chondrocytes during the in vitro cartilage formation process is an important aspect since marker genes associated with developmental aspects are revealed. This can be of further interest when 3D cell cultures need to be optimized for their in vitro performance for the production of new tissue by e.g. adding growth factors that are found to play a major role during the early onset of cartilage formation in vivo.  
      Another experimental setup found in the scope of this invention includes the in vitro culture of cells harvested from cartilaginous areas of arthritic knee joints. Osteoarthritis (OA) results from the failure of chondrocytes within the joint to maintain the balance between synthesis and degradation of extracellular matrix. OA is a multifactorial disorder in which aging, genetic, hormonal and mechanical factors are all major contributors to its onset and progression. With progressing disease state, the articular chondrocytes ability to maintain homeostasis and functionality is increasingly disappearing. As a consequence, the phenotype of osteoarthritic chondrocytes compared with normal chondrocytes exhibits remarkable changes. Gene expression profiling allows characterization of the osteoarthritic cellular phenotype, a key determinant for understanding and manipulation of osteoarthritic processes. By studying and comparing the gene expression profiles of chondrocytes harvested from pathological and healthy human cartilage areas it becomes possible to identify marker genes that are able to predict the future outcome of cell cultures used for in vitro tissue engineering applications. This also relates to the very critical question of the assessment of the quality of the starting biopsy material that is being used for downstream applications like tissue engineering. By having this important information before performing any downstream applications like e.g. proliferation and consecutive 3D in vitro tissue formation, the further steps of any process can then be adapted or even not performed at all because of inadequate quality of biopsy material. Such decision may be of high relevance when tissue-engineering processes are transferred or applied in the clinic. Gene expression profiling of chondrocytes may then be used as a diagnostic tool to allow and to choose that therapeutic approach with the most promising clinical outcome.  
      A further important aspect of the invention is the observation that chondrocytes derived from osteoarthritic patient material always qualify for anchorage dependent proliferation in 2D over several passages. These cells however, if subsequently induced to re-differentiate by culturing them as 3D high density pellets, do not survive over an extended time period, in most cases they die in culture by undergoing apoptosis. It is assumed that these cells, due to an altered phenotype, are not capable of producing the critical survival factors in the appropriate concentrations, above all extracellular matrix components providing intercellular spaces as they occur in native cartilage. Cells that are not suitable to be cultured within 3D high density cultures are herein referred to as “failures”. These impaired cell cultures can be used to set up representative “failure” systems, where cells from different pathological cartilage sources are harvested, proliferated and cultivated in 3D high density pellet culture systems. After each of these experimental steps, RNA can be isolated from the different cell sources and combined to create “failure pools”. These failure pools are very well suitable to identify general marker genes being indicative of the onset of osteoarthritis.  
      For finding cartilage relevant genes, and for determining their presence dependent on the specific cartilage type such as age, health etc., sufficient material must be generated, e.g. by 2D culturing over several passages, and optionally 3D culturing. Said material then can on be subjected to usual gene analyses, and the tissue specific genes determined. Cartillage samples are classified prior to culturing and/or after culturing to get the information needed for later interpretation of the gene expression profile.  
      A further experimental setup of the current inventions discloses the analysis of chondrocytes grown in 3D cultures isolated from pathological human cartilage and analyzed in a time dependent manner. This experimental set-up allows to study the apoptotic process and to further define additional dynamic and characteristic gene expression profiles, useful for deducing and further assessment of the quality of the biopsy material.  
      The microarray process and strategy for disclosing all the cartilage relevant genes with the above-mentioned tissues and cell culture criteria will be described in the following. An important issue of the inovative strategy used by the inventors of the present inventions is to use various microarrays containing a high number of genes comprising different functional categories preferentially by representing the whole genome. The broader the microarray regarding the coverage of the human genome the more genes associated with chondrocyte cell cultures can be determined. The chosen strategy of the inventors was not obvious to a person skilled in the art.  
      RNA isolated from the above mentioned different cell cultures conditions may be radioactive labeled with e.g. 33P or fluorescence like e.g. Cy3 and hybridized to the corresponding filters or microarrays. After hybridization each array may then be scanned and the corresponding signals measured (Tab IV). This raw data file needs then to be calibrated and normalized in a manner to create an input file for the further downstream analysis process. In principle if the data are normalized an expression profile is created. To identify the key cartilage marker genes being differentially expressed under the chosen criteria, tedious bioinformatic analysis are conducted. Corresponding cell cultures and their expression profiles are therefore compared and analyzed accordingly and the different clusters of marker genes determined by software analysis e.g. self-organizing maps (herein referred to as SOM). A representative example of a result for the comparison of different gene expression profiles from different cell culture conditions performed by SOM analysis is given in  FIG. 1 . By performing SOM analysis genes that are similarly expressed are clustered together in so-called sub clusters. The total amount of marker genes for one analysis corresponds to the total amount of sub clusters containing the corresponding genes. Table I in the appendix summarize the results of all the different analysis performed and encompasses all the genes determined for every set of cell culture analysis.  
      By performing this analytical procedure the analysis reveals several characteristic up and down regulated marker genes for different cellular culture conditions. From these marker genes characteristic expression profiles can then be deduced and used as a benchmark for the comparison or further characterization of other cell cultures.  
      Hence, on the one hand previously unknown cartilage—relevant genes associated with different culture conditions and on the other hand characteristic gene expression profiles (cellular fingerprints) indicative of a stage of development, a disease state or a particular selected cell culture condition are revealed. These fingerprints are part of the current invention and are of major importance for the classification and characterization of chondrocytes cultivated under different culture conditions.  
      Since the gene clusters from all the different analysis contain repetitive gene entries, they have been further processed so that only single entry genes are recorded (see Tab II). This 467 selected sequences are thus all key cartilage genes that are activated and thus differentially expressed according to a stage of development, a disease state or a particular selected cell culture condition and are part of the current invention. A list of all 467 genes with their Pubmed accession no. and a description is given below See also Tables II and III):  
      List of Table II Related Sequences:  
                                   Pubmed           Accesion No   Description                  AA283693   Human osteoclast stimulating factor mRNA, complete cds       AA845156   Serine protease inhibitor, Kazal type 1       R52548   Human superoxide dismutase (SOD-1) mRNA, complete cds       T67128   ARYLAMINE N-ACETYLTRANSFERASE, MONOMORPHIC       AA845015   Elastase 1, pancreatic (elastase IIA)       AA937895   Antigen identified by monoclonal antibodies 12E7, F21 and O13       AA844998   Pancreatic polypeptide       AA844818   Amylase, alpha 2A; pancreatic       AA894557   Creatine kinase B       AA872001   Annexin VI (p68)       H09590   Human mRNA for eukaryotic initiation factor 4AI       AA868278   Testis specific protein 1 (probe H4-1 p3-1)       AA490855   Acid finger protein ZNF173       H05820   Human MRL3 mRNA for ribosomal protein L3 homologue (MRL3 = mammalian           ribosome L3)       N57766   Agammaglobulinaemia protein-tyrosine kinase atk       AA873885   Alkaline phosphatase, liver/bone/kidney       AA878880   Interferon (gamma)-induced cell line; protein 10 from       R54818   Human eukaryotic initiation factor 2B-epsilon mRNA, partial cds       AA458630   RENIN PRECURSOR, RENAL       W37864   Phosphatase and tensin homolog (mutated in multiple advanced cancers 1)       N63192   Phenylethanolamine N-methyltransferase       R55789   Human X11 protein mRNA, partial cds       R56871   Human chromatin assembly factor-I p60 subunit mRNA, complete cds       AA448659   M-PHASE INDUCER PHOSPHATASE 2       AA235388   Tropomodulin       W37769   Chromogranin B (secretogranin 1)       AA421701     H. sapiens  mRNA for MUF1 protein       N81029   Collagen, type XVIII, alpha 1       AA644128   Nuclear autoantigenic sperm protein (histone-binding)       N26536   ATPase, Cu++ transporting, beta polypeptide (Wilson disease)       AA890663   Human protein kinase PAK1 mRNA, complete cds       AA405987   Glycerol kinase 2 (testis specific)       AA888182   Ribosomal protein S4, X-linked       H09730   Adenylate kinase 2 (adk2)       AA285155   CDC46 HOMOLOG       AA873351   Ribosomal protein L35a       H12320   CAMP-RESPONSE ELEMENT BINDING PROTEIN       AA856556   Ribosomal protein S28       R43581   Human guanine nucleotide-binding protein G-s, alpha subunit mRNA, partial cds       AA633768   60S RIBOSOMAL PROTEIN L24       AA496880   Ribosomal protein L5       AA625632   Ubiquitin A-52 residue ribosomal protein fusion product 1       R40850     H. sapiens  mRNA for alpha-centractin       AA486072   Small inducible cytokine A5 (RANTES)       N80129   Metallothionein 1L       T67270   UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX SUBUNIT VI REQUIRING           PROTEIN       AA775364   60S RIBOSOMAL PROTEIN L30       AA464743   Ribosomal protein L21       AA663983   Triosephosphate isomerase 1       AA634008   40S RIBOSOMAL PROTEIN S23       AA683050   40S RIBOSOMAL PROTEIN S8       AA775874   60S RIBOSOMAL PROTEIN L18       AA029934   Integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)       AA872397   GALECTIN-2       AA428195   Protein tyrosine phosphatase, non-receptor type 2       AA478724   Insulin-like growth factor binding protein 6       T40541     H. sapiens  mRNA for human giant larvae homolog       N33214     H. sapiens  mRNA for membrane-type matrix metalloproteinase 1       W69399     Homo sapiens  adenosine triphosphatase mRNA, complete cds       H85454     Homo sapiens  delayed-rectifier K+ channel alpha subunit (KCNS1) mRNA, complete           cds       T71284   Complement component 1, q subcomponent, beta polypeptide       N95418   Human FK-506 binding protein homologue (FKBP38) mRNA, complete cds       AA430675   Human DNA repair protein XRCC9 (XRCC9) mRNA, complete cds       AA682851     Homo sapiens  mRNA for ERp28 protein       AA427433   PROTEIN PHOSPHATASE PP2A, 65 KD REGULATORY SUBUNIT, ALPHA           ISOFORM       AA100296     H. sapiens  PAP mRNA       AA070997   Proteasome (prosome, macropain) subunit, beta type, 6       R27585   Proteasome component C2       N71628   Spi-B transcription factor (Spi-1/PU.1 related)       AA464566   Human mRNA for LDL-receptor related protein       AA043228   Calponin 3, acidic       AA478273   APEX nuclease (multifunctional DNA repair enzyme)       H05619     Homo sapiens  GDNF family receptor alpha 2 (GFRalpha2) mRNA, complete cds       AA405562   Protein phosphatase 4 (formerly X), catalytic subunit       AA147043     Homo sapiens  CAGH1a (CAGH1) mRNA, partial cds       AA035384     Homo sapiens  mRNA for small subunit of cytochrome b in succinate dehydrogenase           complex, complete cds       R60150   Human mRNA for histidyl-tRNA synthetase (HRS)       N64051     Homo sapiens  Werner syndrome gene, complete cds       AA405748   SPLICING FACTOR U2AF 65 KD SUBUNIT       AA461110     Homo sapiens  growth-arrest-specific protein (gas) mRNA, complete cds       AA845167   ELASTASE IIIA PRECURSOR       AA443118     Homo sapiens  mRNA for CD151, complete cds       N92319   Glycoprotein Ib (platelet), beta polypeptide       AA187148   Core-binding factor, beta subunit       AA253413   Friedreich ataxia       AA046701   ATP SYNTHASE LIPID-BINDING PROTEIN P1 PRECURSOR       AA164562     Homo sapiens  actin-related protein Arp3 (ARP3) mRNA, complete cds       AA496357     Homo sapiens  SKB1Hs mRNA, complete cds       AA180742   TUBULIN ALPHA-4 CHAIN       AA454743   Human protease M mRNA, complete cds       AA437226   Interleukin 10 receptor       AA458849     Homo sapiens  placental bikunin mRNA, complete cds       AA504891   Crystallin, alpha B       AA609655     Homo sapiens  mRNA for SCP-1, complete cds       AA599158   MULTIFUNCTIONAL AMINOACYL-TRNA SYNTHETASE       AA052932     Homo sapiens  casein kinase I gamma 2 mRNA, complete cds       AA789328     Homo sapiens  (clone PK2J) CDC2-related protein kinase (PISSLRE) mRNA,           complete cds       AA129537   Human GAP SH3 binding protein mRNA, complete cds       AA486209   Low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin           receptor-associated protein 1       H39018     H. sapiens  Syt V gene (genomic and cDNA sequence)       AA464217   V-akt murine thymoma viral oncogene homolog 1       T95053     Homo sapiens  Rigui (RIGUI) mRNA, complete cds       AA454646   LYMPHOTOXIN-BETA RECEPTOR PRECURSOR       AA448400   Human plectin (PLEC1) mRNA, complete cds       H13691   Major histocompatibility complex, class II, DM beta       AA132086     Homo sapiens  RCL (Rcl) mRNA, complete cds       AA488073   Mucin 1, transmembrane       N40945     H. sapiens  mRNA for DRES9 protein       R55705     Homo sapiens  orexin receptor-1 mRNA, complete cds       H50114     Homo sapiens  NMDA receptor mRNA, complete cds       AA452841   Human K-CI cotransporter (hKCC1) mRNA, complete cds       W73790   IMMUNOGLOBULIN-RELATED 14.1 PROTEIN PRECURSOR       N30302   POSSIBLE GTP-BINDING PROTEIN HSR1       AA291556   Human ras inhibitor mRNA, 3′ end       AA598510   Human APRT gene for adenine phosphoribosyltransferase       AA453787   Human TFIIB related factor hBRF (HBRF) mRNA, complete cds       H05655   Human transcriptional activator mRNA, complete cds       AA419177   INTEGRAL MEMBRANE PROTEIN E16       AA458807   Human retinal protein (HRG4) mRNA, complete cds       AA293218   Cleavage stimulation factor, 3′ pre-RNA, subunit 2, 64 kD       W44860   Human calmodulin mRNA, complete cds       AA629862     Homo sapiens  mRNA for smallest subunit of ubiquinol-cytochrome c reductase,           complete cds       AA447674     Homo sapiens  HIV-Nef associated acyl CoA thioesterase (hNAACTE) mRNA,           complete cds       T52484   Nerve growth factor beta       AA496810   Protein kinase C substrate 80K-H       AA486233   G1 to S phase transition 1       AA079775   TYROSINE-PROTEIN KINASE CSK       W73889   Tetranectin (plasminogen-binding protein)       R50337   Solute carrier family 19 (folate transporter), member 1       R55046   MpV17 transgene, murine homolog, glomerulosclerosis       R46821   T-COMPLEX PROTEIN 1, ALPHA SUBUNIT       R87763   Human telencephalin precursor mRNA, complete cds       H69583   Human BTG2 (BTG2) mRNA, complete cds       R56046   Guanine nucleotide binding protein (G protein), alpha z polypeptide       AA922705   Glycogen phosphorylase B (brain form)       AA487571   Surfactant, pulmonary-associated protein C       AA402440     Homo sapiens  exportin t mRNA, complete cds       H29521   ATP-binding cassette 3       AA490911     Homo sapiens  drp1 mRNA, complete cds       AA486082     Homo sapiens  sgk gene       AA678065   2,3-bisphosphoglycerate mutase       R43509   Human Gu binding protein mRNA, partial cds       N57553   Adenosine receptor A2       AA676955   Aplysia ras-related homolog 12       R14692   Human Na/H antiporter (APNH1) mRNA, complete cds       AA488979     Homo sapiens  nucleolar protein (MSP58) mRNA, complete cds       AA443630   Aldehyde dehydrogenase 8       AA027840     H. sapiens  mRNA for RIT protein       AA456830   Diacylglycerol kinase, alpha (80 kD)       AA453015     H. sapiens  L23-related mRNA       AA074446   Human GTP cyclohydrolase I feedback regulatory protein gene, complete cds       AA027042   DNA-DIRECTED RNA POLYMERASE II 23 KD POLYPEPTIDE       AA629923   Human mRNA for pM5 protein       AA460830     Homo sapiens  (clone mf.18) RNA polymerase II mRNA, complete cds       AA454218     Homo sapiens  transcription factor SL1 mRNA, complete cds       AA046523     H. sapiens  mRNA for centrin gene       R51346   Human eIF-2-associated p67 homolog mRNA, complete cds       AA029964   Human ataxin-2 related protein mRNA, partial cds       AA489219   DUTP pyrophosphatase       AA043133   Solute carrier family 16 (monocarboxylic acid transporters), member 1       AA812973   Human mRNA for testis-specific TCP20, complete cds       AA453471   GANGLIOSIDE GM2 ACTIVATOR PRECURSOR       AA284693   Transcription factor AP-4 (activating enhancer-binding protein 4)       N90281   Human B7 mRNA, complete cds       AA629542   Brush-1       AA679345   Human BTK region clone ftp-3 mRNA       H37774   Tuberin       T97181   Platelet factor 4       AA454879   Plasminogen activator, urokinase receptor       AA147640   Phosphorylase, glycogen; liver (Hers disease, glycogen storage disease type VI)       AA757429   Human serotonin N-acetyltransferase mRNA, complete cds       AA490991     Homo sapiens  HnRNP F protein mRNA, complete cds       AA422058     H. sapiens  mRNA for D1075-like gene       N66208   Human (ard-1) mRNA, complete cds       AA630776   Human AP-3 complex delta subunit mRNA, complete cds       AA827287   Human interferon-induced leucine zipper protein (IFP35) mRNA, partial cds       AA488084   Superoxide dismutase 2, mitochondrial       R89715   Protein kinase C, gamma       AA490501     H. sapiens  mRNA; UV Radiation Resistance Associated Gene       N32199   Human melanoma antigen recognized by T-cells (MART-1) mRNA       AA434404   DNA primase polypeptide 2A (58 kD)       N93686   Aldehyde dehydrogenase 7       AA292676   Human metargidin precursor mRNA, complete cds       AA464417   INTERFERON-INDUCIBLE PROTEIN 1-8U       AA442092   Catenin (cadherin-associated protein), beta 1 (88 kD)       AA026644   Transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)       AA481464   Peptidylprolyl isomerase B (cyclophilin B)       T68859   Alpha-2-plasmin inhibitor (alpha-2-PI)       AA699560   Surfeit 1       AA705069   Human mRNA for receptor of retinoic acid       AA457739     Homo sapiens  putative OSP like protein mRNA, partial cds       H99843     Homo sapiens  mRNA for quinolinate phosphoribosyl transferase, complete cds       AA399410   Signal transducer and activator of transcription 3 (acute-phase response factor)       AA443039   HEAT SHOCK 70 KD PROTEIN 1       AA164440   Human autoantigen pericentriol material 1 (PCM-1) mRNA, complete cds       AA446453   Human mRNA for c-myc binding protein, complete cds       AA280692   Diacylglycerol kinase delta       AA031514   Matrix metalloproteinase 7 (matrilysin, uterine)       R33154   Msh ( Drosophila ) homeo box homolog 1 (formerly homeo box 7)       AA487452   Human DNA fragmentation factor-45 mRNA, complete cds       AA400329   Human gene for neurofilament subunit M (NF-M)       AA454668   Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and           cyclooxygenase)       AA486393   Cytokine receptor family II, member 4       R52541   unknown EST       AA171613     Homo sapiens  carbonic anhydrase precursor (CA 12) mRNA, complete cds       AA235706   Human TATA-binding protein associated factor 30 kDa subunit (tafII30) mRNA,           complete cds       AA668527   Human mucosal addressin cell adhesion molecule-1 (MAdCAM-1) mRNA, complete           cds       T54144     Homo sapiens  homolog of the  Aspergillus nidulans  sudD gene product mRNA,           complete cds       R14080   Calcium modulating ligand       AA609599     Homo sapiens  SSX3 (SSX3) mRNA, complete cds       AA489201     H. sapiens  mRNA for PHAPI2b protein       R08876   Human 26S proteasome-associated pad1 homolog (POH1) mRNA, complete cds       H46425     H. sapiens  Pur (pur-alpha) mRNA, complete cds       R56149   Human putative transmembrane protein (nma) mRNA, complete cds       AA454619     Homo sapiens  mRNA for Hic-5, partial cds       H15445     H. sapiens  mRNA for SEX gene       AA705225   Myosin, light polypeptide 4, alkali; atrial, embryonic       AA191488   Human high-affinity copper uptake protein (hCTR1) mRNA, complete cds       N64862   Human SLP-76 associated protein mRNA, complete cds       R45413   Human transmembrane 4 superfamily protein (SAS) mRNA, complete cds       R77293   Intercellular adhesion molecule 1 (CD54), human rhinovirus receptor       AA436187   Integrin, alpha M (complement component receptor 3, alpha; also known as CD11b           (p170), macrophage antigen alpha polypeptide)       AA676470     H. sapiens  IAI.3B mRNA       AA443634     Homo sapiens  ubiquitin conjugating enzyme G2 (UBE2G2) mRNA, complete cds       AA664180   Glutathione peroxidase 3 (plasma)       W58658     H. sapiens  mRNA for CLPP       H54023     Homo sapiens  monocyte/macrophage Ig-related receptor MIR-10 (MIR cl-10) mRNA,           complete cds       H73724   Cyclin-dependent kinase 6       T70031   Human neutral amino acid transporter B mRNA, complete cds       AA481758   DNAJ PROTEIN HOMOLOG 1       AA521431   Human profilin mRNA, complete cds       AA446103   ERGIC-53 PROTEIN PRECURSOR       N92646   Immunoglobulin gamma 3 (Gm marker)       AA453789   Protein-tyrosine kinase 7       AA425299     Homo sapiens  ezrin-radixin-moesin binding phosphoprotein-50 mRNA, complete cds       AA868929   Troponin T1, skeletal, slow       R60019   Homolog 2 of  Drosophila  large discs       AA857343   Human putative RNA binding protein (RBP56) mRNA, complete cds       AA481438   Complement component 1 inhibitor (angioedema, hereditary)       AA399674   Human small proline rich protein (sprII) mRNA, clone 1292       T98887   Glucose-6-phosphatase       AA676404   Peptidylprolyl isomerase C (cyclophilin C)       H15747   Human HU-K4 mRNA, complete cds       H16958   Human glyceraldehyde 3-phosphate dehydrogenase mRNA       AA936783   Eukaryotic translation initiation factor 3 (eIF-3) p36 subunit       AA884709   Cytochrome P450 11 beta       H24688   Human SWI/SNF complex 170 KDa subunit (BAF170) mRNA, complete cds       AA884403   Human cardiotrophin-1 (CTF1) mRNA, complete cds       AA404619   5′ nucleotidase (CD73)       AA598611   IMMEDIATE-EARLY RESPONSE PROTEIN NOT       H72875   GATA-binding protein 3       H63361   Eukaryotic translation initiation factor 2B (eIF-2B) alpha subunit       R39221   Human MAP kinase mRNA, complete cds       R02346   U1 snRNP 70K protein       R51835   unknown EST       R33031     H. sapiens  mRNA for sigma 3B protein       AA412053   CD9 antigen       AA001897   Erythroid alpha-spectrin       W81191     Homo sapiens  nucleolar autoantigen No55 mRNA, complete cds       AA430552     Homo sapiens  proline-rich Gla protein 2 (PRGP2) mRNA, complete cds       AA394130   Human transducin-like protein mRNA, complete cds       N92864   Human cleavage and polyadenylation specificity factor mRNA, complete cds       AA457123   VALYL-TRNA SYNTHETASE       R43320   Human guanine nucleotide-binding regulatory protein (Go-alpha) gene       AA670430   Glutamate receptor, metabotropic 3       H65066   Visinin-like 1       AA458785   GUANYLATE CYCLASE SOLUBLE, BETA-1 CHAIN       AA485871     H. sapiens  mRNA for myosin-I beta       T39411   Human 53K isoform of Type II phosphatidylinositol-4-phosphate 5-kinase (PIPK)           mRNA, complete cds       R00855     Homo sapiens  59 protein mRNA, 3′ end       H98666   Metallopeptidase 1 (33 kD)       H72028   GELSOLIN PRECURSOR, PLASMA       AA679177   Human follistatin-related protein precursor mRNA, complete cds       N21576   Human mitochondrial 1,25-dihydroxyvitamin D3 24-hydroxylase mRNA, complete cds       AA007419   Human RGP4 mRNA, complete cds       T49657     Homo sapiens  TWIK-related acid-sensitive K+ channel (TASK) mRNA, complete cds       N38959     Homo sapiens  chaperonin containing t-complex polypeptide 1, beta subunit (Cctb)           mRNA, complete cds       R51912   Human somatostatin I gene and flanks       H90415   Breast cancer 1, early onset       H41489   Adaptin, beta 1 (beta prime)       H15456   CALPAIN 1, LARGE       W45415   ELASTASE IIIB PRECURSOR       AA447751   Tyrosine hydroxylase       AA487486   Cyclin D1 (PRAD1; parathyroid adenomatosis 1)       R56604   Cholinergic receptor, nicotinic, alpha polypeptide 4       T65772   pulmonary surfactant protein (SP5)       H15085   ADP-ribosylation factor 4-like       R61295   Human ADP/ATP translocase mRNA, 3′ end, clone pHAT8       T61256     H. sapiens  KHK mRNA for ketohexokinase, clone pHKHK3a       AA405731   Phosphoenolpyruvate carboxykinase 1 (soluble)       T71879   Complement component C2       R59927   Human mRNA for cytochrome c oxidase subunit VIc       AA496780   Human small GTP binding protein Rab7 mRNA, complete cds       AA176688   Human mRNA for lysosomal sialoglycoprotein, complete cds       AA436163     Homo sapiens  Pig12 (PIG12) mRNA, complete cds       AA428778   Human placenta LERK-2 (EPLG2) mRNA, complete cds       AA463225   Bone morphogenetic protein 4       AA485426   Interferon (alpha, beta and omega) receptor 2       W47485   Human sigma receptor mRNA, complete cds       H84982   Human checkpoint suppressor 1 mRNA, complete cds       AA504615     Homo sapiens  mRNA for CAB1, complete cds       H94487   Cathepsin E       AA448959     Homo sapiens  NADH: ubiquinone oxidoreductase 15 kDa IP subunit mRNA, nuclear           gene encoding mitochondrial protein, complete cds       AA070358   Transketolase (Wernicke-Korsakoff syndrome)       AA453401   Human PH-20 homolog (LUCA2) mRNA, partial cds       N66737   Collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia,           congenital)       AA666180   Human v-erbA related ear-2 gene       AA857131   Human Tat-SF1 mRNA, complete cds       AA479102   Protein kinase C, beta 1       AA456077     Homo sapiens  mRNA for p27, complete cds       R87497     H. sapiens  mRNA for 2.19 gene       AA718910   Human tax1-binding protein TXBP181 mRNA, complete cds       AA406269   Nuclear factor I/X (CCAAT-binding transcription factor)       N74623   Insulin-like growth factor 2 (somatomedin A)       H99364   Human chloride channel protein (CLCN7) mRNA, partial cds       AA447684   Small proline-rich protein 1B (cornifin)       AA282301     Homo sapiens  nuclear dual-specificity phosphatase (SBF1) mRNA, partial cds       H99588   Human lymphoid nuclear protein (LAF-4) mRNA, complete cds       N53512     Homo sapiens  alpha 2 delta calcium channel subunit isoform I mRNA, complete cds       AA683321     Homo sapiens  PAR-5 mRNA, probable 5′ end       AA608557   Damage-specific DNA binding protein 1 (127 kD)       AA757764     Homo sapiens  mRNA for DNA-binding protein, complete cds       AA406064     Homo sapiens  testis-specific Basic Protein Y 1 (BPY1) mRNA, complete cds       N54596   Human Krueppel-related zinc finger protein (H-plk) mRNA, complete cds       AA481988   Transcription factor 7 (T-cell specific)       N62394   Gap junction protein, beta 1, 32 kD (connexin 32, Charcot-Marie-Tooth neuropathy, X-           linked)       N26148   Zinc finger protein 148 (pHZ-52)       AA496678   B-cell CLL/lymphoma 3       AA400973   NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN PRECURSOR       AA497027   Human mRNA, clone HH109 (screened by the monoclonal antibody of insulin           receptor substrate-1 (IRS-1))       N64508     Homo sapiens  podocalyxin-like protein mRNA, complete cds       AA033564     H. sapiens  mRNA for DGCR6 protein       AA446108   Endoglin (Osler-Rendu-Weber syndrome 1)       AA159577   Mucin 5, subtype B, tracheobronchial       R36958   unknown EST       AA629808   Ribosomal protein L6       AA482067   Human tazarotene-induced gene 2 (TIG2) mRNA, complete cds       AA669314   ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit       AA775241   Aldolase A       R73584     Homo sapiens  hydroxysteroid sulfotransferase SULT2B1a (HSST2) mRNA, complete           cds       H28984   PHOSPHATIDYLSERINE SYNTHASE I       R44202     Homo sapiens  catechol-O-methyltransferase (COMT) mRNA, complete cds       W70051     H. sapiens  mRNA for M-phase phosphoprotein, mpp9       AA401972   Human RalGDS-like 2 (RGL2) mRNA, partial cds       AA236164   CATHEPSIN S PRECURSOR       R22412   Platelet/endothelial cell adhesion molecule (CD31 antigen)       AA424804   MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 1       AA669443   Eukaryotic translation initiation factor 5 (elF5)       N69689   RAS-RELATED PROTEIN RAB-1A       H24316   AQUAPORIN-CHIP       AA074224   Recoverin       R36571   Human U1 snRNP-specific protein A gene       AA056465   Human 54 kDa protein mRNA, complete cds       AA633811     H. sapiens  E4BP4 gene       AA457155   Human zinc-finger protein C2H2-150 mRNA, complete cds       AA459104   60S RIBOSOMAL PROTEIN L13       R40212   Human coatomer protein (HEPCOP) mRNA, complete cds       AA086476   Adenosine monophosphate deaminase 1 (isoform M)       AA663310   Thymidylate synthase       AA455640     Homo sapiens  signalosome subunit 3 (Sgn3) mRNA, complete cds       AA496879   Human (clone E5.1) RNA-binding protein mRNA, complete cds       AA085749     Homo sapiens  mRNA for ATP binding protein, complete cds       AA425755     Homo sapiens  mRNA for leukemia associated gene 1       N52350     H. sapiens  mRNA for protein-tyrosine-phosphatase (tissue type: testis)       AA630104   Lipase A, lysosomal acid, cholesterol esterase (Wolman disease)       AA454854   ALPHA-AMYLASE 2B PRECURSOR       W73406   DIHYDROPRYRIDINE-SENSITIVE L-TYPE, SKELETAL MUSCLE CALCIUM           CHANNEL GAMMA SUBUNIT       R12802   Human cytochrome bc-1 complex core protein II mRNA, complete cds       AA465355     Homo sapiens  mRNA for U3 snoRNP associated 55 kDa protein       AA829383   DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 3       AA629189   Keratin 4       AA430512     Homo sapiens  cytoplasmic antiproteinase 3 (CAP3) mRNA, complete cds       AA456439   Human homozygous deletion target in pancreatic carcinoma (DPC4) mRNA,           complete cds       H27864   SECRETOGRANIN II PRECURSOR       AA644657   MHC class I protein HLA-A (HLA-A28, -B40, -Cw3)       R40460     Homo sapiens  phosphatidylinositol 4-kinase mRNA, complete cds       W96058   Human hnRNP H mRNA, complete cds       T72202   Human transcription factor IL-4 Stat mRNA, complete cds       AA598794   Connective tissue growth factor       AA599178   Ribosomal protein L27a       R88247   Adrenergic, beta, receptor kinase 1       T98612   Alpha-1 type 3 collagen       AA454856   Phospholipid hydroperoxide glutathione peroxidase       N67048   Type 3 iodothyronine deiodinase       AA778675     Homo sapiens  mRNA for calmegin, complete cds       H51117   Human calmodulin dependent phosphodiesterase PDE1B1 mRNA, complete cds       N36174   5-HYDROXYTRYPTAMINE 2B RECEPTOR       AA777187     Homo sapiens  Cyr61 mRNA, complete cds       R09561   Decay accelerating factor for complement (CD55, Cromer blood group system)       R16849   Human HsPex13p mRNA, complete cds       AA884167   ANNEXIN XIII       AA136983   Cadherin 11 (OB-cadherin)       AA488622   Human signal transducing adaptor molecule STAM mRNA, complete cds       AA699427   Fructose-bisphosphatase 1       AA490459   Transcobalamin II       AA626787   Human ras-related C3 botulinum toxin substrate (rac) mRNA, complete cds       N62179   Human methylmalonate semialdehyde dehydrogenase gene, complete cds       N27190   UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L3       AA441895   Human glutathione-S-transferase homolog mRNA, complete cds       AA463924   FACTOR VIII INTRON 22 PROTEIN       N78843     Homo sapiens  cyclophilin-33A (CYP-33) mRNA, complete cds       AA629719   Cytochrome c oxidase VIIc subunit       AA464755   Ankyrin 1, erythrocytic       AA459351     H. sapiens  sds22-like mRNA       AA488346   MYOSIN LIGHT CHAIN ALKALI, SMOOTH-MUSCLE ISOFORM       AA427899   Human mRNA fragment encoding beta-tubulin. (from clone D-beta-1)       AA453813     H. sapiens  mRNA for Gal-beta(1-3/1-4)GlcNAc alpha-2.3-sialyltransferase       AA397824   Dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)       AA633901   Transforming growth factor, beta-induced, 68 kD       AA181334   Troponin I (skeletal fast)       AA292410   Clusterin (complement lysis inhibitor; testosterone-repressed prostate message 2;           apolipoprotein J)       AA253434   HEAT SHOCK FACTOR PROTEIN 2       AA455056     H. sapiens  mRNA for MAP kinase activated protein kinase       R55188   Human pre-T/NK cell associated protein (3B3) mRNA, 3′ end       AA465723     Homo sapiens  mRNA for protein phosphatase 2C gamma       N49856   SODIUM-AND CHLORIDE-DEPENDENT BETAINE TRANSPORTER       AA455272     H. sapiens  mRNA for ITBA1 protein       AA459292   CDC28 protein kinase 1       AA878561   Ubiquitin A-52 residue ribosomal protein fusion product 1       AA772066   Human phosphatidylinositol (4,5)bisphosphate 5-phosphatase homolog mRNA,           partial cds       N78621     H. sapiens  mRNA for gamma-adaptin       AA291490     H. sapiens  mRNA for processing a-glucosidase I       N46828     Homo sapiens  mRNA for inositol 1,4,5-trisphosphate 3-kinase isoenzyme, partial cds       AA150487   Alkaline phosphatase, placental (Regan isozyme)       AA282537   MYOCYTE-SPECIFIC ENHANCER FACTOR 2       AA707922   Human mRNA for cone-specific cGMP phosphodiesterase gamma subunit, complete           cds       AA443638     Homo sapiens  breast cancer-specific protein 1 (BCSG1) mRNA, complete cds       W73892   Human putative tumor suppressor (LUCA15) mRNA, complete cds       N70734   Troponin T2 (cardiac)       H57136   Human phospholemman chloride channel mRNA, complete cds       AA709414   Nidogen (enactin)       W65461   Human protein tyrosine phosphatase mRNA, complete cds       AA436564   Human cellular proto-oncogene (c-mer) mRNA, complete cds       AA029042   Human hSIAH2 mRNA, complete cds       AA427725     Homo sapiens  carboxypeptidase Z precursor, mRNA, complete cds       N51280   ADP-ribosylation factor like 1       AA281347     H. sapiens  mRNA for MHC class I promoter binding protein       AA402960   Human HLA class III region containing NOTCH4 gene, partial sequence, homeobox           PBX2 (HPBX) gene, receptor for advanced glycosylation end products (RAGE) gene,           complete cds, and 6 unidentified cds       N98485   Human forkhead protein FREAC-2 mRNA, partial cds       AA490209     H. sapiens  mRNA for Sop2p-like protein       W61361     Homo sapiens  cytoplasmic antiproteinase 2 (CAP2) mRNA, complete cds       N51018   Biglycan       AA455281   DEFENDER AGAINST CELL DEATH 1       W69471   V-ski avian sarcoma viral oncogene homolog       AA486321   Vimentin       AA458982   Solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+,           amiloride sensitive)       AA442095   NEDD-4 PROTEIN       N99003   Active BCR-related gene       AA609284     Homo sapiens  mRNA for Eph-family protein, complete cds       AA195036   Human Ro/SSA ribonucleoprotein homolog (RoRet) mRNA, complete cds       AA478268   Human CtBP mRNA, complete cds       AA608583     Homo sapiens  mRNA for OTK27, complete cds       AA486435     Homo sapiens  mRNA for CDEP, complete cds       AA505045   Human L2-9 transcript of unrearranged immunoglobulin V(H)5 pseudogene       AA487893   TUMOR-ASSOCIATED ANTIGEN L6       AA292226     Homo sapiens  creatine transporter mRNA, complete cds       H87106     Homo sapiens  T245 protein (T245) mRNA, complete cds       W96450   Human putative tRNA synthetase-like protein mRNA, complete cds       N33331   Human peroxisome proliferator activated receptor mRNA, complete cds       AA405800   Dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)       T51539   Macrophage stimulating 1 (hepatocyte growth factor-like)       N59764   Human guanosine 5′-monophosphate synthase mRNA, complete cds       AA521346     H. sapiens  mRNA for Ndr protein kinase       AA428551     Homo sapiens  SOX22 protein (SOX22) mRNA, complete cds       AA489383   Bone morphogenetic protein 2       AA490172   Collagen, type I, alpha-2       AA504477   Human cytoskeleton associated protein (CG22) mRNA, complete cds                  
 
      List of Table III related sequences:  
                                       Accession —                 mrgd   NAME   Gene                  M98539   Human prostaglandin D2 synthase gene, exon 7           AB004922     Homo sapiens  gene for Smad 3, exon 1, partial sequence       AB006000     Homo sapiens  mRNA for chondromodulin-I precursor, complete cds       AB017364     Homo sapiens  mRNA for frizzled-2, complete cds       AB020236     Homo sapiens  gene for ribosomal protein L27A, complete cds   Ribosomal protein L27a       AB042820     Homo sapiens  RPL6 gene for ribosomal protein L6, complete cds   Ribosomal protein L6       AB043547     Homo sapiens  gene for SMAD4, partial cds       AB080265     Homo sapiens  CYP2J2 mRNA for cytochrome P450 2J2, complete cds   Cytochrome P450, subfamily IIJ               (arachidonic acid epoxygenase) polypeptide 2       AF000979     Homo sapiens  testis-specific Basic Protein Y 1 (BPY1) mRNA, complete cds     Homo sapiens  testis-specific Basic Protein               Y 1 (BPY1) mRNA, complete cds       AF001450     Homo sapiens  core binding factor alpha1           subunit (CBFA1) gene, exon 7 and complete cds       AF004231     Homo sapiens  monocyte/macrophage Ig-related receptor MIR-10 (MIR cl-10)     Homo sapiens  monocyte/macrophage Ig-related           mRNA, complete cds   receptor MIR-10 (MIR cl-10) mRNA,               complete cds       AF009801     Homo sapiens  homeodomain protein (BAPX1) mRNA, complete cds       AF010126     Homo sapiens  breast cancer-specific protein 1 (BCSG1) mRNA, complete cds     Homo sapiens  breast cancer-specific               protein 1 (BCSG1) mRNA, complete cds       AF010316     Homo sapiens  Pig12 (PIG12) mRNA, complete cds     Homo sapiens  Pig12 (PIG12) mRNA,               complete cds       AF013591     Homo sapiens  homolog of the Aspergillus nidulans sudD gene product mRNA,     Homo sapiens  homolog of the           complete cds     Aspergillus nidulans  sudD gene product               mRNA, complete cds       AF037204     Homo sapiens  RING zinc finger protein (RZF) mRNA, complete cds       AF043339     Homo sapiens  macrophage           inflammatory protein 1 alpha (MIP1a) mRNA, partial cds       AF049656     Homo sapiens  inducible nitric oxide synthase (iNOS) mRNA, complete cds       AF072872     Homo sapiens  frizzled 1 mRNA, complete cds       AF188285     Homo sapiens  bone morphogenetic protein 9 (BMP9) mRNA, complete cds       AF189279     Homo sapiens  group IIE secretory phospholipase A2 mRNA, complete cds       AF248634     Homo sapiens  syndecan 3 (SDC3) mRNA, complete cds       AF304431     Homo sapiens  hypoxia-inducible factor           1 alpha subunit (HIF1A) mRNA, complete cds       AF339054     Homo sapiens  BCL2-associated X protein (BAX) gene, exons 1, 2 and partial cds       AF348700     Homo sapiens  ubiquitin A-52 residue ribosomal protein fusion product 1   Ubiquitin A-52 residue ribosomal protein           (UBA52), mRNA, complete cds   fusion product 1       AF395008     Homo sapiens  interleukin 4 (IL4) gene, complete cds       AF405705     Homo sapiens  matrix metalloproteinase 3           (stromelysin 1, progelatinase) (MMP3) gene, complete cds       AF411526     Homo sapiens  nerve growth factor beta (NGFB) mRNA, complete cds   Nerve growth factor beta       AF469046     Homo sapiens  macrophage migration inhibitory factor (MIF) mRNA, complete cds       AF477981     Homo sapiens  osterix mRNA, complete cds       AJ279016     Homo sapiens  mRNA for chondrocyte expressed protein 68 kDa (CEP-68 gene)       AY043326     Homo sapiens  keratin 4 (KRT4) gene, complete cds   Keratin 4       AY044847     Homo sapiens  aggrecanase 1 (ADAMTS4) gene, complete cds       D13748   Human mRNA for eukaryotic initiation factor 4AI   Human mRNA for eukaryotic               initiation factor 4AI       D38255     Homo sapiens  mRNA for CAB1, complete cds     Homo sapiens  mRNA for CAB1, complete cds       D45399   Human mRNA for cone-specific cGMP phosphodiesterase gamma subunit,   Human mRNA for cone-specific cGMP           complete cds   phosphodiesterase gamma subunit,               complete cds       D49738   Human cytoskeleton associated protein (CG22) mRNA, complete cds   Human cytoskeleton associated protein               (CG22) mRNA, complete cds       D49835     Homo sapiens  mRNA for DNA-binding protein, complete cds     Homo sapiens  mRNA for DNA-binding               protein, complete cds       D90040   Human mRNA for arylamine N-acetyltransferase (EC 2.3.1.5)   ARYLAMINE N-ACETYLTRANSFERASE,               MONOMORPHIC       J00306   Human somatostatin I gene and flanks   Human somatostatin I gene and flanks       J03191   Human profilin mRNA, complete cds   Human profilin mRNA, complete cds       J03592   Human ADP/ATP translocase mRNA, 3′ end, clone pHAT8   Human ADP/ATP translocase mRNA,               3′ end, clone pHAT8       J04111   Human c-jun proto oncogene (JUN), complete cds, clone hCJ-1       J04177   Human alpha-1 type XI collagen (COL11A1) mRNA, complete cds       J04973   Human cytochrome bc-1 complex core protein II mRNA, complete cds   Human cytochrome bc-1 complex core               protein II mRNA, complete cds       J05036   Human cathepsin E mRNA, complete cds   Cathepsin E       K00065   Human superoxide dismutase (SOD-1) mRNA, complete cds   Human superoxide dismutase (SOD-1) mRNA,               complete cds       K00650   Human fos proto-oncogene (c-fos), complete cds       L05095     Homo sapiens  ribosomal protein L30 mRNA, complete cds   60S RIBOSOMAL PROTEIN L30       L08895     Homo sapiens  MADS/MEF2-family           transcription factor (MEF2C) mRNA, complete cds       L10347   Human pro-alpha1 type II collagen (COL2A1) gene exons 1-54, complete cds       L11566     Homo sapiens  ribosomal protein L18 (RPL18) mRNA, complete cds   60S RIBOSOMAL PROTEIN L18       L13286   Human mitochondrial 1,25-dihydroxyvitamin D3 24-hydroxylase mRNA,   Human mitochondrial 1,25-dihydroxyvitamin           complete cds   D3 24-hydroxylase mRNA, complete cds       L13463   Human helix-loop-helix basic phosphoprotein (G0S8) mRNA, complete cds       L13616   Human focal adhesion kinase (FAK) mRNA, complete cds       L13720     Homo sapiens  growth-arrest-specific protein (gas) mRNA, complete cds     Homo sapiens  growth-arrest-specific protein               (gas) mRNA, complete cds       L22009   Human hnRNP H mRNA, complete cds   Human hnRNP H mRNA, complete cds       L28997     Homo sapiens  ARL1 mRNA, complete cds   ADP-ribosylation factor like 1       L31409     Homo sapiens  creatine transporter mRNA, complete cds     Homo sapiens  creatine transporter mRNA,               complete cds       L33930     Homo sapiens  CD24 signal transducer mRNA, complete cds and 3′ region       L34059     Homo sapiens  cadherin-4 mRNA, complete cds       L41162     Homo sapiens  collagen alpha 3 type IX (COL9A3) mRNA, complete cds       L47647     Homo sapiens  creatine kinase B mRNA, complete cds   Creatine kinase B       M13994   Human B-cell leukemia/lymphoma 2 (bcl-2) proto-oncogene           mRNA encoding bcl-2-alpha protein, complete cds       M14144   Human vimentin gene, complete cds   Vimentin       M14631   Human guanine nucleotide-binding protein G-s, alpha subunit mRNA, partial   Human guanine nucleotide-binding protein G-s,           cds   alpha subunit mRNA, partial cds       M16652   Human pancreatic elastase IIA mRNA, complete cds   Elastase 1, pancreatic (elastase IIA)       M20137   Human interleukin 3 (IL-3) mRNA, complete cds, clone pcD-SR-alpha       M22636   Human U1 small nuclear ribonucleoprotein 70 kd protein mRNA, complete cds   U1 snRNP 70K protein       M37825   Human fibroblast growth factor-5 (FGF-5) mRNA, complete cds       M57293   Human parathyroid hormone-related peptide           (PTHRP) gene, exons 1A, 1B, 1C, and 2       M58458   Human ribosomal protein S4 (RPS4X) isoform mRNA, complete cds   Ribosomal protein S4, X-linked       M58525     Homo sapiens  catechol-O-methyltransferase (COMT) mRNA, complete cds     Homo sapiens  catechol-O-methyltransferase               (COMT) mRNA, complete cds       M58549   Human matrix Gla protein (MGP) mRNA, complete cds       M61877   Human erythroid alpha-spectrin (SPTA1) mRNA, complete cds   Erythroid alpha-spectrin       M62402   Human insulin-like growth factor binding protein 6 (IGFBP6) mRNA, complete   Insulin-like growth factor binding protein 6           cds       M65062   Human insulin-like growth factor binding protein 5 (IGFBP-5) mRNA, complete cds       M65217   Human heat shock factor 2 (HSF2) mRNA, complete cds   HEAT SHOCK FACTOR PROTEIN 2       M76701     Homo sapiens  zinc finger protein 35 (ZNF35) gene, exon 1       M77016   Human tropomodulin mRNA, complete cds   Tropomodulin       M81768   Human Na/H antiporter (APNH1) mRNA, complete cds   Human Na/H antiporter (APNH1) mRNA,               complete cds       M84489   Human extracellular signal-regulated kinase 2 mRNA, complete cds       M84721   Human AMP deaminase (AMPD3) mRNA, complete cds   Adenosine monophosphate deaminase               (isoform E)       M87842   Human S-lac lectin L-14-II (LGALS2) mRNA, complete cds   GALECTIN-2       M92934   Human connective tissue growth factor, complete cds   Connective tissue growth factor       M95610   Human alpha 2 type IX collagen (COL9A2) mRNA, partial cds       M96684     H. sapiens  Pur (pur-alpha) mRNA, complete cds     H. sapiens  Pur (pur-alpha) mRNA,               complete cds       M97676     Homo sapiens  (region 7) homeobox protein (HOX7) mRNA, complete cds       NM_000194     Homo sapiens  hypoxanthine phosphoribosyltransferase           1 (Lesch-Nyhan syndrome) (HPRT1), mRNA       NM_000213     Homo sapiens  integrin, beta 4 (ITGB4), mRNA       NM_000221     Homo sapiens  ketohexokinase (fructokinase) (KHK), transcript variant a,     H. sapiens  KHK mRNA for ketohexokinase,           mRNA   clone pHKHK3a       NM_000235     Homo sapiens  lipase A, lysosomal acid, cholesterol esterase (Wolman   Lipase A, lysosomal acid, cholesterol esterase           disease) (LIPA), mRNA   (Wolman disease)       NM_000358     Homo sapiens  transforming growth factor, beta-induced, 68 kD (TGFBI), mRNA   Transforming growth factor,               beta-induced, 68 kD       NM_000364     Homo sapiens  troponin T2, cardiac (TNNT2), mRNA   Troponin T2 (cardiac)       NM_000537     Homo sapiens  renin (REN), mRNA   RENIN PRECURSOR, RENAL       NM_000574     Homo sapiens  decay accelerating factor for complement (CD55, Cromer blood   Decay accelerating factor for complement           group system) (DAF), mRNA   (CD55, Cromer blood group system)       NM_000600     Homo sapiens  interleukin 6 (interferon, beta 2) (IL6), mRNA       NM_000618     Homo sapiens  insulin-like growth factor 1 (somatomedia C) (IGF1), mRNA       NM_000632     Homo sapiens  Integrin, alpha M (complement component receptor 3, alpha;   Integrin, alpha M (complement component           also known as CD11b (p170), macrophage antigen alpha polypeptide)   receptor 3, alpha; also known as CD11b           (ITGAM), mRNA   (p170), polypeptide) macrophage antigen alpha       NM_000711     Homo sapiens  bone gamma-carboxyglutamate           (gla) protein (osteocalcin) (BGLAP), mRNA       NM_000962     Homo sapiens  prostaglandin-endoperoxide synthase 1 (prostaglandin G/H   Prostaglandin-endoperoxide synthase 1           synthase and cyclooxygenase) (PTGS1), transcript variant 1, mRNA   (prostaglandin G/H synthase and               cyclooxygenase)       MM_000977     Homo sapiens  ribosomal protein L13 (RPL13), transcript variant 1, mRNA   60S RIBOSOMAL PROTEIN L13       NM_000996     Homo sapiens  ribosomal protein L35a (RPL35A), mRNA   Ribosomal protein L35a       NM_001012     Homo sapiens  ribosomal protein S8 (RPS8), mRNA   40S RIBOSOMAL PROTEIN S8       NM_001025     Homo sapiens  ribosomal protein S23 (RPS23), mRNA   40S RIBOSOMAL PROTEIN S23       NM_001064     Homo sapiens  transketolase (Wernicke-Korsakoff syndrome) (TKT), mRNA   Transketolase (Wernicke-Korsakoff syndrome)       NM_001127     Homo sapiens  adaptor-related protein complex 1, beta 1 subunit   Adaptin, beta 1 (beta prime)               AP1B1), mRNA       NM_001200     Homo sapiens  bone morphogenetic protein 2 (BMP2), mRNA   Bone morphogenetic protein 2       NM_001229     Homo sapiens  caspase 9, apoptosis-related cysteine protease           (CASP9), transcript variant alpha, mRNA       NM_001511     Homo sapiens  GRO1 oncogene (melanoma growth           stimulating activity, alpha) (GRO1), mRNA       NM_001565     Homo sapiens  small inducible cytokine subfamily B (Cys-X-Cys), member 10   Interferon (gamma)-induced cell line;           (SCYB10), mRNA   protein 10 from       NM_001632     Homo sapiens  alkaline phosphatase, placental (Regan isozyme) (ALPP),   Alkaline phosphatase, placental           mRNA   (Regan isozyme)       NM_001687     Homo sapiens  ATP synthase, H+ transporting, mitochondrial F1 complex, delta   ATP synthase, H+ transporting,           subunit (ATP5D), mRNA   mitochondrial F1 complex, delta subunit       NM_001718     Homo sapiens  bone morphogenetic protein 6 (BMP6), mRNA       NM_001745     Homo sapiens  calcium modulating ligand (CAMLG), mRNA   Calcium modulating ligand       NM_001797     Homo sapiens  cadherin 11, type 2, OB-cadherin           (osteoblast) (CDH11), transcript variant 1, mRNA       NM_001844     Homo sapiens  collagen, type II, alpha 1 (primary osteoarthritis,           spondyloepiphyseal dysplasia, congenital) (COL2A1), transcript variant 1, mRNA       NM_001912     Homo sapiens  cathepsin L (CTSL), mRNA       NM_001969     Homo sapiens  eukaryotic translation initiation factor 5 (EIF5), mRNA   Eukaryotic translation initiation factor 5 (eIF5)       NM_002073     Homo sapiens  guanine nucleotide binding protein (G protein), alpha z   Guanine nucleotide binding protein           polypeptide (GNAZ), mRNA   polypeptide (G protein), alpha z       NM_002094     Homo sapiens  G1 to S phase transition 1 (GSPT1), mRNA   G1 to S phase transition 1       NM_002160     Homo sapiens  hexabrachion (tenascin C, cytotactin) (HXB), mRNA       NM_002211     Homo sapiens  integrin, beta 1 (fibronectin receptor, beta polypeptide,           antigen CD29 includes MDF2, MSK12) (ITGB1), mRNA       NM_002379     Homo sapiens  matrilin 1, cartilage matrix protein (MATN1), mRNA       NM_002381     Homo sapiens  matrilin 3 (MATN3) precursor, mRNA       NM_002421     Homo sapiens  matrix metalloproteinase 1 (interstitial collagenase) (MMP1), mRNA       NM_002424     Homo sapiens  matrix metalloproteinase 8 (neutrophil collagenase) (MMP8), mRNA       NM_002427     Home sapiens  matrix metalloproteinase 13 (collagenase 3) (MMP13), mRNA       NM_002591     Homo sapiens  phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1), mRNA   Phosphoenolpyruvate carboxykinase 1 (soluble)           mRNA carboxykinase 1 (soluble)       NM_002619     Home sapiens  platelet factor 4 (PF4), mRNA   Platelet factor 4       NM_002722     Homo sapiens  pancreatic polypeptide (PPY), mRNA       NM_002738     Homo sapiens  protein kinase C, beta 1 (PRKCB1), mRNA   Protein kinase C, beta 1       NM_002903     Homo sapiens  recoverin (RCV1), mRNA   Recoverin       NM_003036     Homo sapiens  v-ski sarcoma viral oncogene homolog (avian) (SKI), mRNA   V-ski avian sarcoma viral oncogene homolog       NM_003282     Homo sapiens  troponin I, skeletal, fast (TNNI2), mRNA   Troponin I (skeletal fast)       NM_003385     Homo sapiens  visinin-like 1 (VSNL1),   Visinin-like 1       NM_003395     Homo sapiens  wingless-type MMTV integration           site family, member 14 (WNT14), mRNA       NM_004613     Homo sapiens  transglutaminase 2 (C polypeptide,           protein-glutamine-gamma-glutamyltransferase) (TGM2), mRNA       NM_004832     Homo sapiens  glutathione-S-transferase like; glutathione transferase omega   Human glutathione-S-transferase homolog           (GSTTLp28), mRNA   cds mRNA, complete       NM_004994     Homo sapiens  matrix metalloproteinase 9 (gelatinase B, 92 kD gelatinase,           92 kD type IV collagenase) (MMP9), mRNA       NM_004995     Homo sapiens  matrix metalloproteinase 14 (membrane-inserted) (MMP14), mRNA       NM_005038     Homo sapiens  peptidylprolyl isomerase D (cyclophilin D) (PPID), mRNA   40 KD PEPTIDYL-PROLYL CIS-TRANS               ISOMERASE       NM_005186     Homo sapiens  calpain 1, (mu/l) large subunit (CAPN1), mRNA   CALPAIN 1, LARGE       NM_005346     Homo sapiens  heat shock 70 kD protein 1B (HSPA1B), mRNA   HEAT SHOCK 70 KD PROTEIN 1       NM_005438     Homo sapiens  FOS-like antigen 1 (FOSL1), mRNA       NM_005506     Homo sapiens  CD36 antigen (collagen type I receptor, thrombospondin           receptor)-like 2 (lysosomal integral membrane protein II) (CD36L2), mRNA       NM_006289     Homo sapiens  talin 1 (TLN1), mRNA       NM_006988     Homo sapiens  a disintegrin-like and metalloprotease (reprolysin type)           with thrombospondin type 1 motif, 1 (ADAMTS1), mRNA       NM_007306     Homo sapiens  breast cancer 1, early onset (BRCA1), transcript variant   Breast cancer 1, early onset           BRCA1-exon4, mRNA       NM_007352     Homo sapiens  elastase 3B, pancreatic (ELA3B), Mrna   ELASTASE IIIB PRECURSOR       NM_014000     Homo sapiens  vinculin (VCL), transcript variant meta-VCL, mRNA       NM_014470     Homo sapiens  GTP-binding protein (RHO6), mRNA       NM_018952     Homo sapiens  homeo box B6 (HOXB6), mRNA       NM_021019     Homo sapiens  myosin, light polypeptide 6, alkali, smooth muscle and non-   MYOSIN LIGHT CHAIN ALKALI,           muscle (MYL6), transcript variant 1, mRNA   SMOOTH-MUSCLE ISOFORM       NM_033150     Homo sapiens  collagen, type II, alpha 1 (primary osteoarthritis,           spondyloepiphyseal dysplasia, congenital) (COL2A1), transcript variant 2, mRNA       NM_053056     Homo sapiens  cyclin D1 (PRAD1: parathyroid adenomatosis 1) (CCND1),   Cyclin D1 (PRAD1; parathyroid           mRNA   adenomatosis 1)       NM_080682     Homo sapiens  vascular cell adhesion           molecule 1 (VCAM 1), transcript variant 2, mRNA       S79854     Homo sapiens  type 3 iodothyronine deiodinase mRNA, complete cds   Type 3 iodothyronine deiodinase       S83308   SOX5 = Sry-related HMG box gene {alternatively           spliced} [human, testis, mRNA, 1473 nt]       U07424   Human putative tRNA synthetase-like protein mRNA, complete cds   Human putative tRNA synthetase-like               protein mRNA, complete cds       U07620   Human MAP kinase mRNA, complete cds   Human MAP kinase mRNA, complete cds       U08023   Human cellular proto-oncogene (c-mer) mRNA, complete cds   Human cellular proto-oncogene (c-mer)               mRNA, complete cds       U09303   Human T cell leukemia LERK-2 (EPLG2) mRNA, complete cds       U09577     Homo sapiens  lysosomal hyaluronidase (LUCA2/HYAL2) mRNA, complete cds   Human PH-20 homolog (LUCA2) mRNA,               partial cds       U09825   Human acid finger protein mRNA, complete cds   Acid finger protein ZNF173       U13261     Homo sapiens  eIF-2-associated p67 homolog mRNA, complete cds   Human eIF-2-associated p67 homolog               mRNA, complete cds       U13660   Human cartilage-derived morphogenetic protein 1 (CDMP-1) mRNA, complete cds       U13991   Human TATA-binding protein associated factor 30 kDa subunit (tafII30) mRNA,   Human TATA-binding protein associated           complete cds   factor 30 kDa subunit (tafII30) mRNA,               complete cds       U14966   Human ribosomal protein L5 mRNA, complete cds   Ribosomal protein L5       U14971   Human ribosomal protein S9 mRNA, complete cds       U15085   Human HLA-DMB mRNA, complete cds   Major histocompatibility complex,               class II, DM beta       U16031   Human transcription factor IL-4 Stat mRNA, complete cds   Human transcription factor IL-4 Stat               mRNA, complete cds       U16261   Human MDA-7 (mda-7) mRNA, complete cds       U18299   Human damage-specific DNA binding protein DDBa p127 subunit (DDB1)   Damage-specific DNA binding protein           mRNA, complete cds   1 (127 kD)       U20980   Human chromatin assembly factor-I p60 subunit mRNA, complete cds   Human chromatin assembly factor-I               p60 subunit mRNA, complete cds       U22409   Human parathyroid hormone/PTH-related peptide receptor           (PTH/PTHrP) gene, exon 14 and complete cds       U23028   Human eukaryotic initiation factor 2B-epsilon mRNA, partial cds   Human eukaryotic initiation factor               2B-epsilon mRNA, partial cds       U23946   Human putative tumor suppressor (LUCA15) mRNA, complete cds   Human putative tumor suppressor               (LUCA15) mRNA, complete cds       U24152   Human p21-activated protein kinase (Pak1) gene, complete cds   Human protein kinase PAK1               mRNA, complete cds       U25789   Human ribosomal protein L21 mRNA, complete cds   Ribosomal protein L21       U27699   Human pephBGT-1 betaine-GABA transporter mRNA, complete cds   SODIUM-AND CHLORIDE-DEPENDENT               BETAINE TRANSPORTER       U31202   Human noggin (NOGGIN) gene, complete cds, (NOG)       U32169   Human pro-a2 chain of collagen type XI (COL11A2) gene, complete cds       U32907   Human p37NB mRNA, complete cds   Human p37NB mRNA, complete cds       U33822   Human tax1-binding protein TXBP181 mRNA, complete cds   Human tax1-binding protein TXBP181               mRNA, complete cds       U37012   Human cleavage and polyadenylation specificity factor mRNA, complete cds   Human cleavage and polyadenylation               specificity factor mRNA, complete cds       U38864   Human zinc-finger protein C2H2-150 mRNA, complete cds   Human zinc-finger protein C2H2-150               mRNA, complete cds       U40373   Human cell surface glycoprotein CD44 mRNA, complete cds       U41517   Human channel-like integral membrane protein (AQP-1) mRNA, clone AQP-1-   AQUAPORIN-CHIP           1656, complete cds       U43148   Human patched homolog (PTC) mRNA, complete cds       U43747   Human frataxin (FRDA) mRNA, complete cds   Friedreich ataxia       U43842     Homo sapiens  bone morphogenetic protein-4 (hBMP-4) gene, complete cds   Bone morphogenetic protein 4       U45975   Human phosphatidylinositol (4,5)bisphosphate 5-phosphatase homolog mRNA,   Human phosphatidylinositol           partial cds   (4,5)bisphosphate 5- phosphatase homolog               mRNA, partial cds       U53204   Human plectin (PLEC1) mRNA, complete cds   Human plectin (PLEC1) mRNA, complete cds       U53347   Human neutral amino acid transporter B mRNA, complete cds   Human neutral amino acid transporter B               mRNA, complete cds       U59289   Human H-cadherin mRNA, complete cds       U59423   Human Smad1 mRNA, complete cds       U63717     Homo sapiens  osteoclast stimulating factor mRNA, complete cds   Human osteoclast stimulating factor               mRNA, complete cds       U68723   Human checkpoint suppressor 1 mRNA, complete cds       U70312     Homo sapiens  integrin binding protein Del-1 (Del1) mRNA, complete cds       U72245   Human phospholemman chloride channel mRNA, complete cds   Human phospholemman chloride channel               mRNA, complete cds       U75283   Human sigma receptor mRNA, complete cds   Human sigma receptor mRNA, complete cds       U76992   Human Tat-SF1 mRNA, complete cds   Human Tat-SF1 mRNA, complete cds       U80998   Human basic helix-loop-helix DNA binding protein (TWIST) gene, complete cds       U83460   Human high-affinity copper uptake protein (hCTR1) mRNA, complete cds   Human high-affinity copper uptake protein               (hCTR1) mRNA, complete cds       U90547   Human Ro/SSA ribonucleoprotein homolog (RoRet) mRNA, complete cds   Human Ro/SSA ribonucleoprotein homolog               (RoRet) mRNA, complete cds       U92268     Homo sapiens  mitogen activated protein kinase p38-2 mRNA, complete cds       U93181     Homo sapiens  nuclear dual-specificity phosphatase (SBF1) mRNA, partial cds     Homo sapiens  nuclear dual-specificity               phosphatase (SBF1) mRNA, partial cds       X00129   Human mRNA for retinol binding protein (RBP)       X00588   Human mRNA for precursor of epidermal growth factor receptor       X02910   Human gene for tumor necrosis factor (TNF-alpha)       X03742   Human gene for L apoferritin exons 1 and 2       X04412   Human mRNA for plasma gelsolin   GELSOLIN PRECURSOR, PLASMA       X06614   Human mRNA for receptor of retinoic acid   Human mRNA for receptor of retinoic acid       X12794   Human v-erbA related ear-2 gene   Human v-erbA related ear-2 gene       X14420   Human mRNA for pro-alpha-1 type 3 collagen       X51801   Human OP-1 mRNA for osteogenic protein       X54412   Human mRNA for alpha1(IX) collagen (long form)       X55654     Homo sapiens  mitochondrial coxII mRNA for cytochrome C oxidase II subunit       X55764   Human mRNA for cytochrome P-450 (11 Beta)   Cytochrome P450 11 beta       X58399   Human L2-9 transcript of unrearranged immunoglobulin V(H)5 pseudogene   Human L2-9 transcript of unrearranged               immunoglobulin V(H)5 pseudogene       X58957     H. sapiens  atk mRNA for agammaglobulinaemia tyrosine kinase   Agammaglobulinaemia protein-tyrosine               kinase atk       X60188   Human ERK1 mRNA for protein serine/threonine kinase       X60382     H. sapiens  COL10A1 gene for collagen (alpha-1 type X)       X67337     H. sapiens  HPBRII-4 mRNA     H. sapiens  HPBRII-4 mRNA       X70683     H. sapiens  mRNA for SOX-4 protein       X71661     H. sapiens  ERGIC-53 mRNA   ERGIC-53 PROTEIN PRECURSOR       X74795     H. sapiens  P1-Cdc46 mRNA   CDC46 HOMOLOG       X76770     H. sapiens  PAP mRNA     H. sapiens  PAP mRNA       X78712     H. sapiens  mRNA for glycerol kinase testis specific 2   Glycerol kinase 2 (testis specific)       X87237     H. sapiens  mRNA for processing a-glucosidase I     H. sapiens  mRNA for processing               a-glucosidase I       X87342     H. sapiens  mRNA for human giant larvae homolog     H. sapiens  mRNA for human               giant larvae homolog       X92475     H. sapiens  mRNA for ITBA1 protein     H. sapiens  mRNA for ITBA1 protein       X94216     H. sapiens  mRNA for VEGF-C protein       XM_001306     Homo sapiens  solute carrier family 16 (monocarboxylic acid transporters),   Solute carrier family 16 (monocarboxylic           member 1 (SLC16A1), mRNA   acid transporters), member 1       XM_001316     Homo sapiens  adenosine monophosphate deaminase 1 (isoform M) (AMPD1),   Adenosine monophosphate           mRNA   deaminase 1 (isoform M)       XM_001324     Homo sapiens  calponin 3, acidic (CNN3), mRNA   Calponin 3, acidic       XM_001782     Homo sapiens  fibromodulin (FMOD), mRNA       XM_001826     Homo sapiens  alkaline phosphatase, liver/bone/kidney (ALPL), mRNA   Alkaline phosphatase, liver/bone/kidney       XM_002321     Homo sapiens  glypican 1 (GPC1), mRNA   Glypican 1       XM_003059     Homo sapiens  peroxisome proliferative           activated receptor, gamma (PPARG), mRNA       XM_003222     Homo sapiens  catenin (cadherin-associated           protein), beta 1 (88 kD) (CTNNB1), mRNA       XM_003730     Homo sapiens  cytochrome c oxidase subunit VIIc (COX7C), mRNA   Cytochrome c oxidase VIIc subunit       XM_003752     Homo sapiens  interleukin 3 (colony-stimulating factor, multiple) (IL3), mRNA       XM_003913     Homo sapiens  integrin, alpha 2 (CD49B, alpha           2 subunit of VLA-2 receptor) (ITGA2), mRNA       XM_004063     Homo sapiens  early growth response 1 (EGR1), mRNA       XM_006121     Homo sapiens  cathepsin D (lysosomal aspartyl protease) (CTSD), mRNA       XM_009336     Homo sapiens  cartilage oligomeric matrix protein (pseudoachondroplasia,           epiphyseal dysplasia 1, multiple) (COMP), mRNA       XM_009915     Homo sapiens  leukemia inhibitory factor           (cholinergic differentiation factor) (LIF), mRNA       XM_010702     Homo sapiens  cathepsin K (pycnodysostosis) (CTSK), mRNA       XM_012503     Homo sapiens  matrix metalloproteinase 2 (gelatinase A, 72 kD           gelatinase, 72 kD type IV collagenase) (MMP2), mRNA       XM_012651     Homo sapiens  collagen, type I, alpha 1 (COL1A1), mRNA       XM_015434     Homo sapiens  chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1), mRNA       XM_016181     Homo sapiens  wingless-type MMTV           integration site family, member 5A (WNT5A), mRNA       XM_017096     Homo sapiens  active BCR-related gene (ABR), mRNA       XM_017384     Homo sapiens  matrix metalloproteinase 7 (matrilysin, uterine) (MMP7), mRNA   Matrix metalloproteinase 7 (matrilysin, uterine)       XM_017591     Homo sapiens  annexin A6 (ANXA6), mRNA   Annexin VI (p68)       XM_028204     Homo sapiens  nuclear factor of kappa light polypeptide gene           enhancer in B-cells 1 (p105) (NFKB1), mRNA       XM_028642     Homo sapiens  integrin, alpha 5           (fibronectin receptor, alpha polypeptide) (ITGA5), mRNA       XM_029245     Homo sapiens  collagen, type I, alpha 2 (COL1A2), mRNA   Collagen, type I, alpha-2       XM_029796     Homo sapiens  frizzled-related protein (FRZB), mRNA       XM_031221     Homo sapiens  interleukin 1, alpha (IL1A), mRNA       XM_031288     Homo sapiens  aggrecan 1 (chondroitin sulfate proteoglycan 1, large           aggregating proteoglycan, antigen identified by monoclonal           antibody A0122) (AGC1), mRNA       XM_031289     Homo sapiens  interleukin 8 (IL8), mRNA       XM_032902     Homo sapiens  integrin, alpha 1 (ITGA1), mRNA       XM_033470     Homo sapiens  defender against cell death 1 (DAD1), mRNA   DEFENDER AGAINST CELL DEATH 1       XM_033657     Homo sapiens  heparan sulfate proteoglycan 2 (perlecan) (HSPG2), mRNA       XM_033878     Homo sapiens  tissue inhibitor of metalloproteinase 1 (erythroid potentiating           activity, collagenase inhibitor) (TIMP1), mRNA       XM_034023     Homo sapiens  regulator of G-protein signalling 4 (RGS4), mRNA   Human RGP4 mRNA, complete cds       XM_034556     Homo sapiens  chloride channel 7 (CLCN7), mRNA       XM_034845     Homo sapiens  phosphatase and tensin homolog (mutated in multiple advanced   Phosphatase and tensin homolog           cancers 1) (PTEN), mRNA   (mutated in multiple advanced cancers 1)       XM_034890     Homo sapiens  fibrillin 1 (Marfan syndrome) (FBN1), mRNA       XM_035662     Homo sapiens  cathepsin B (CTSB), mRNA       XM_035842     Homo sapiens  small inducible cytokine A5 (RANTES) (SCYA5), mRNA   Small inducible cytokine A5 (RANTES)       XM_036107     Homo sapiens  integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated           antigen 1; macrophage antigen 1 (mac-1) beta subunit) (ITGB2), mRNA       XM_036175     Homo sapiens  collagen, type XVIII, alpha 1 (COL18A1), mRNA   Collagen, type XVIII, alpha 1       XM_037087     Homo sapiens  ATP binding protein associated with cell differentiation     Homo sapiens  mRNA for ATP binding           (APACD), mRNA   protein, complete cds       XM_037646     Homo sapiens  msh homeo box homolog 2 ( Drosophila ) (MSX2), mRNA       XM_037965     Homo sapiens  chondroadherin (CHAD), mRNA       XM_038584     Homo sapiens  tissue inhibitor of metalloproteinase 3           (Sorsby fundus dystrophy, pseudoinflammatory) (TIMP3), mRNA       XM_039094     Homo sapiens  SRY (sex determining region Y)-box 9           (campomelic dysplasia, autosomal sex-reversal) (SOX9), mRNA       XM_040037     Homo sapiens  adrenergic, beta, receptor kinase 1 (ADRBK1), mRNA   Adrenergic, beta, receptor kinase 1       XM_040385     Homo sapiens  S-adenosylmethionine decarboxylase 1 (AMD1), mRNA   S-adenosylmethionine decarboxylase 1       XM_042153     Homo sapiens  biglycan (BGN), mRNA   Biglycan       XM_042664     Homo sapiens  nuclear autoantigenic sperm protein (histone-binding) (NASP),   Nuclear autoantigenic sperm           mRNA   protein (histone-binding)       XM_044120     Homo sapiens  fibroblast growth factor receptor 3           (achondroplasia, thanatophoric dwarfism) (FGFR3), mRNA       XM_045089     Homo sapiens  ATPase, Cu++ transporting, beta polypeptide (Wilson disease)   ATPase, Cu++ transporting,           (ATP7B), mRNA   beta polypeptide (Wilson disease)       XM_045802     Homo sapiens  paxillin (PXN), Mrna       XM_045890     Homo sapiens  ADP-ribosylation factor 4-like (ARF4L), mRNA   ADP-ribosylation factor 4-like       XM_045925     Homo sapiens  decorin (DCN), mRNA   Decorin       XM_045926     Homo sapiens  lumican (LUM), mRNA       XM_046035     Homo sapiens  integrin, alpha L (antigen CD11A (p180), lymphocyte function-   Integrin, alpha L (antigen           associated antigen 1; alpha polypeptide) (ITGAL), mRNA   CD11A (p180), lymphocyte function-associated               antigen 1; alpha polypeptide)       XM_046758     Homo sapiens  tensin (TNS), mRNA       XM_046765     Homo sapiens  thymidylate synthetase (TYMS), mRNA   Thymidylate synthase       XM_047231     Homo sapiens  fibulin 1 (FBLN1), mRNA       XM_047719     Homo sapiens  transcription factor 7 (T-cell specific, HMG-box) (TCF7), mRNA   Transcription factor 7 (T-cell specific)       XM_047802     Homo sapiens  a disintegrin-like and metalloprotease (reprolysin type)           with thrombospondin type 1 motif, 5 (aggrecanase-2) (ADAMTS5), mRNA       XM_048167     Homo sapiens  troponin T1, skeletal, slow (TNNT1), mRNA   Troponin T1, skeletal, slow       XM_048201     Homo sapiens  metallothionein 1L (MT1L), mRNA   Metallothionein 1L       XM_049177     Homo sapiens  vascular endothelial growth factor B (VEGFB), mRNA       XM_049518     Homo sapiens  intercellular adhesion molecule 1 (CD54), human rhinovirus   Intercellular adhesion molecule           receptor (ICAM1), mRNA   receptor 1 (CD54), human rhinovirus       XM_049534     Homo sapiens  amylase, alpha 2A; pancreatic (AMY2A), mRNA   Amylase, alpha 2A; pancreatic       XM_049690     Homo sapiens  coatomer protein complex, subunit alpha (COPA), mRNA   Human coatomer protein (HEPCOP)               mRNA, complete cds       XM_049864     Homo sapiens  colony stimulating factor 3 (granulocyte) (CSF3), mRNA       XM_049937     Homo sapiens  insulin-like growth factor binding protein 4 (IGFBP4), mRNA       XM_050846     Homo sapiens  Indian hedgehog homolog ( Drosophila ) (IHH), mRNA       XM_053809     Homo sapiens  similar to chondroitin sulfate proteoglycan 2(versican)           ( H. sapiens ) (LOC153633), mRNA       XM_054566     Homo sapiens  collagen, type VI, alpha 1 (COL6A1), mRNA       XM_054686     Homo sapiens  caspase 3, apoptosis-related cysteine protease (CASP3), mRNA       XM_055254     Homo sapiens  fibronectin 1 (FN1), mRNA       XM_058069     Homo sapiens  matrix metalloproteinase 12 (macrophage elastase) (MMP12), mRNA       XM_084239     Homo sapiens  retinoic acid receptor responder (tazarotene induced) 2   Human tazarotene-induced           (RARRES2), Mrna   gene 2 (TIG2) mRNA, complete cds       XM_084263     Homo sapiens  cytochrome c oxidase subunit VIc (COX6C), mRNA   Human mRNA for               cytochrome c oxidase subunit VIc       XM_084285     Homo sapiens  integral membrane protein 2A (ITM2A), mRNA       XM_085705     Homo sapiens  tissue inhibitor of metalloproteinase 2 (TIMP2), mRNA       XM_086368     Homo sapiens  MUF1 protein (MUF1), mRNA     H. sapiens  mRNA for MUF1 protein       XM_096277     Homo sapiens  collagen, type V, alpha 1 (COL5A1), mRNA       Y00985   Human mRNA for manganese-containing superoxide dismutase       Y07566     H. sapiens  mRNA for RIT protein     H. sapiens  mRNA for RIT protein       Y07570     H. sapiens  mRNA for PHAPI2b protein     H. sapiens  mRNA for PHAPI2b protein       Y08999     H. sapiens  mRNA for Sop2p-like protein     H. sapiens  mRNA for Sop2p-like protein       Y12692     Homo sapiens  mRNA for WNT11 gene       Y13936     Homo sapiens  mRNA for protein phosphatase 2C gamma     Homo sapiens  mRNA for               protein phosphatase 2C gamma       Y15227     Homo sapiens  mRNA for leukemia associated gene 1     Homo sapiens  mRNA for               leukemia associated gene 1       Z22865     H. sapiens  dermatopontin mRNA, complete CDS       Z50781     H. sapiens  mRNA for leucine zipper protein       Z50853     H. sapiens  mRNA for CLPP     H. sapiens  mRNA for CLPP                  
 
      The current invention also encompasses the process of down compression of previously determined 467 genes to a lower number that is still able to characterize the desired number of different cellular status. At present, for the determination of 7 different cell types or development stages, a minimum of 26 spots of different marker genes are preferred, much preferred about 200 such spots. For full information, at least one spot for each of the presently 467 genes (markers) is preferred. A reduction of spot number can be of relevance e.g. if under certain conditions only a small subset of those genes listed in Tab II is required for analysis e.g. in clinical applications. This down compression can be achieved by determining the ratio of actual to target number of genes and then choosing from each cluster accordingly to the determined ratio the necessary number of genes to fulfill the requirement. This process requires to group the number of genes for each analysis of e.g. Tab. I into representative cluster familys from where representative genes can be selected. Such clusters familys can be determined as shown in  FIG. 1 , namely by grouping clusters together that show a similar expression pattern. For each cluster family a representative number of genes may be choosen according to the compression factor that has been defined. It can easily be seen that for larger clusters like e.g. “A” in Tab I more genes are available to select while in other clusters like e.g. “E” in Tab I less are present. At the end of the process one needs to balance the procedure in order to preserve the characteristics of the expression profile. In order to do so the amount of genes for each analysis should at least be greater than 2 sequences or spots, respectively, of different genes and for the total array at least 30. In order to control such a process classical hierarchical clustering (Stanford) analysis can be performed and checked on graphical presentations like treeview (Stanford). Cluster analysis may group similar expression profiles in families and will allow distinguishing between different cell sources and allows classification of these cell cultures (see  FIG. 2 ). If the cell sources are not properly represented in the cluster analysis it means that the selected marker genes are not balanced.  
      Example of an Cartilage Specific Micro Array Structure:  
      To produce a microarray with printed oligonucleotides sequences of approx. at least 10 mers, preferably at least 25 mers, some sequences of table II need to be further processed. Since some of the determined sequences in Tab II are only expressed sequence tags (herein referred to as EST), they do not correspond to or represent the full-length cDNA. Therefore the EST preferably is BLAST searched with the public database at NCBI and the corresponding full-length cDNA determined. Only by having the correct and full-length cDNA it is possible to design oligomeric sequences that are balanced to each other and minimize any cross reactivity. Exemplary polynucleotide sequences (targets) are provided in the sequence listing of Table III. The cartilage related polynucleotide sequences as e.g. listed in Table III and other polynucleotide sequences known as key cartilage genes from the literature can be immobilized on a substrate and used as hybridizable array elements in a microarray format. Such microarrays can be composed of a subset of oligonucleotides representing e.g. sequences listed on Tab. II but modified to represent only full-length cDNA sequences. The used polynucleotides for the production of such a microarray can either be 50mer or also PCR (polymerase chain reaction) products but at least need to be longer then 10 bases. It should be noted that for microarray production also PCR products from the corresponding determined sequences directly or the full length cDNA can be used and it is not restricted just to oligonucleotides.  
      Methods to anchor such oligonucleotides or polynucleotides on a solid support are described in literature, together with information on length dependent distances between each oligo or polynucleotides and spots. (see e.g. Principal and Practice, DNA microarrays: gene expression analysis B. Jordan, Springer, 2001)  
      When polynucleotides are employed as hybridizable array elements in a microarray and depending on the software used, the array elements may be organized in an ordered fashion so that each element is present at a specified location on the substrate. If the array elements are at specified locations on the substrate, the hybridization patterns and intensities (which together create a unique expression profile) can be interpreted in terms of expression levels of particular genes. This expression profile can then be used and may be correlated with any effect associated with a tissue and/or compound or to be investigated with regard to a specific tissue and/or compound and allows comparison with already existing data.  
      One of such useful application of using ordered polynucleotides on microarrays is e.g. the comparison of gene expression profiles from a new sample e.g. a tissue biopsy, with already determined characteristic gene expression profiles that are preferably stored in a database. Such stored gene expression profiles are e.g. of major importance if microarrays are applied in the clinic. In this case advantageously a database is set up that stores the corresponding gene expression profiles and advantageously also all patient informations, e.g. history, blood pressure etc. By including all patient data and gene expression profiles in the analysis process and then starting a comparison with an expression profile from a new biopsy, it becomes possible to achieve a stronger correlation with the clinical outcome. This will allow to determine which therapy shall be applied, or even to modify an existing therapy, e.g. to add growth factor x at a concentration y during the ex vivo tissue engineering phase. It may also be the case that the biopsy sample will demonstrate a poor gene expression profile that precludes the successful application of a modern therapeutic cell/tissue approach. Such cases would then only qualify for traditional surgical approaches, and hence would not obtain the benefits of the tissue engineering process.  
      In analogy, the assessment of in vitro produced cartilage can also be performed. In the same way as mentioned above cell culture parameters, like e.g. culture media conditions, growth factor concentration, are preferably stored in a data base together with the corresponding gene expression profiles. Comparison of the database entry with new profiles of new samples can then be used to assess the quality of the new in vitro produced tissue.  
      Subject Arrays and Their Use:  
      It should be noted that the invention described here is not dependent on any special array format rather than the possibility to select from an extended list of 467 novel key cartilage genes as well as meaningful gene expression patterns. A presently preferred subject array is a novel cartilage specific microarray that includes 187 genes that in the scope of this invention have been determined to be cartilage related and 140 genes that have been connected to cartilage in literature (see also Tab II). Normally, in high-density array procedures up to 10000 genes are usually applied and are not specific for certain applications. As one major general drawback, this results in massive data overflow and impaired data analysis due to difficult data handling and procedures. A preferred array has in its current state a minimal number of 150 genes, presently much preferred at most 333 genes, all of those with demonstrated relevance within cartilage tissue. Another major limitation has become apparent. While the invention WO01/24833 A2 describes a few marker genes associated with cartilage phenotype stability they do not allo w to extensively describe chondrocyte cultures in details. No comprehensive classification of the different cell populations and culture conditions is possible as well as no gene expression profile or fingerprint can be achieved. Gene expression profiles determined with a set of genes represented in Tab II may allow to perform a more comprehensive analysis of different cell cultures conditions. Furthermore it may allow to compare and classify different tissue or the result of the different applied cell culture conditions. The above mentioned topics may only be possible with the disclosed invention as outlined within the following applications.  
      The inventive array CART-CHIP 300 ™ may be applied to classify (quality control) any source material, such as human cartilage biopsies, mesenchymal stem cell containing bone marrow aspirate, or pre-chondrogenic cells containing tissue according to pre-defined categories with respect to their capacity to re-build or re-organize a hyaline cartilage-like matrix in vitro. A rough subdivision could be for example “A”, “B”, or “C”. While “A” will easily produce cartilage-like matrix, “B” will require special treatment to achieve an implantable construct, and “C” will represent those cases that do yet not qualify for such a procedure. This biopsy classification system will allow: 
          Quality control of the starting biopsy material and therefore optimization of the downstream process regarding e.g. in vitro tissue engineering applications     Diagnostic evaluation of the patient and candidate treatment methods (e.g. CARTIGRAF™) to ensure a cost-optimized procedure     Quality control of in vitro tissue engineered products        

      The subject array of the present invention can be employed for all kind of research and developmental studies related to in vitro tissue engineering of cartilage. The possibility to assess proliferation, differentiation or re-differentiation as well as de novo matrix formation processes through analyses and comparison of a plurality of key cartilage genes (positive/negative markers) within one single experiment replaces current trial and error approaches and is thus far more rational.  
      The subject array can be applied to screen all kind of drugs, e.g. hormones, growth factors, within in vitro chondrocyte cultures regarding a potential beneficial effect on proliferation, differentiation, de novo matrix formation. The deduced expression profiles can then be compared with existing data of e.g. native cartilage tissue and used to further optimize the process. Additionally the expression profiles can be compared with data from human adult and human infant cartilage to deduce a pathway or a strategy of how to induce more tissue formation in vitro.  
      The subject array of the preferred embodiment is very well suitable to better understand reaction pathways leading to new responses of chondrocytes in vitro. Only key cartilage genes comprising the whole spectrum of functional gene categories are to be investigated. This can be used to study the complexity of degenerative cartilage process in vitro and the respective influence of potential beneficial drugs.  
      The subject array may be used to optimize cultures for in vitro cartilage formation starting from human cell sources other than cartilage like e.g. mesenchymal stem cells or bon marrow aspirates.  
      This subject array will be preferably used as powerful alternative for conventional molecular biology tools beside more established histological and biochemical analyses. By focusing on the most prominent cartilage marker genes being either positive or negative, it is possible to characterize cartilage or cartilage related tissues as well as cell cultures thereof. In this respect, the subject array can replace conventional RT-PCR studies performed to check for cartilage marker gene expression, e.g. collagen I versus collagen II, aggrecan versus versican. By applying this subject array the set of markers will be easily increased by simultaneously simplifying the experimental procedure and enhancing the outcome.  
      The subject arrays of the present invention have several advantages compared to existing microarrays as well as to conventional gene expression tools such as RT-PCR, Northern Blots etc.  
      Most importantly, the subject arrays are all based on key cartilage genes. Beyond all the key cartilage genes known from the literature (˜100-200 genes), 467 additional cartilage relevant genes have been discovered. Thus a significantly increased pool of cartilage key genes exists to choose from for various applications. For instance, to understand degenerative processes as they occur in OA or RA by study of complex biological reaction pathways, it is important to follow expression of a relatively large number of genes.  
     EXAMPLES  
      The examples are described for the purposes of illustration and are not intended to limit the scope of the invention.  
     Example 1  
     Analysis of Various Human Cartilage Samples  
      Useful for characterizing chondrocyte cultures derived from different human cartilage samples (adult and fetal), where adult samples are different with respect to their capacity to form living tissue engineered equivalents under high density culture conditions.  
      Adult chondrocytes show different gene expression clusters compared to fetal chondrocytes and can be further distinguished from samples that will not produce living cartilage constructs (failures).  
      Human chondrocytes from adult and fetal articular cartilage were proliferated in DMEM-F12 medium containing 10% FCS over several passages and transferred to pellet cultures (0.5*10 6  cells) in serum free DMEM-F12 medium supplemented with Ascorbate and Insulin medium. Proliferated cells were directly lyzed with RLT buffer (RNeasy® Mini Kit, Qiagen) after trypsin release from plastic substrate, shredded (QIAshredder, Qiagen) and kept frozen at −80° C. in lysis buffer for later processing. High density pellet cultures were cultivated for 2 weeks if not otherwise specified, subsequently washed with phosphate buffered saline (PBS) and lyzed in RLT Buffer (supplied with RNeasy® Kit). Total RNA was isolated from all samples as described in the manual provided with the RNeasy kit and stored at −80° C.  
      Fluorescent labeled aRNA (amplified RNA) constructs were obtained by in vitro reverse transcription of the RNA followed by an in vitro amplification reaction.  
      2 μg of isolated total RNA were used per sample to amplify RNA by applying only one cycle of in vitro transcription (IVT, Millenium Biologix AG, Application Note).  
      2 μg of total RNA from each sample was primed with oligo(dT) 24-mer  (containing a T7 RNA Polymerase Promotor) and reverse transcribed using 400 Units SuperScript II reverse transcriptase enzyme, nucleotides, 5× Reaction Buffer and Dithiothreithol (DTT) as described in protocol provided with the enzyme. For ribonuclease protection 1 μL RNase inhibitor (10 Units) was used to prevent RNA degradation during first strand synthesis. This first strand synthesis reaction was incubated for 1 hour at 42° C.  
      To the first strand synthesis reaction 93 μl nuclease free water, 30 μl second strand buffer (Invitrogen, Basel, Switzerland) and 1.5 μl nuclotide mix (dATP, dTTP, dGTP, dCTP, 25 mM each) was added.  
      Second strand synthesis reaction mix was obtained by adding 40 Units  E. coli  polymerase I (New England Biolabs, BioConcept, Allschwil, Switzerland), 10 Units  E. coli  DNA Ligase (New England Biolabs, BioConcept, Allschwil, Switzerland) and 2.5 Units Ribonuclease H (Fermentas, Labforce AG, Nunningen, Switzerland). Reaction was incubated for 2 hours at 16° C.  
      After this incubation step remaining RNA was degraded by adding 7.5 μl 1M sodium hydroxid containing 2 mM EDTA (Ethylenediaminetetraacetic acid) for 10 minutes at 65° C. 7.5 μl 1M Hydrochloric acid was added to neutralize the reaction.  
      The obtained double strand DNA was purified in a QIAquick® PCR purification kit (Qiagen, Hilden, Germany) and concentrated to 7.5 μl. To this concentrated RNA following reagents were added to obtain aRNA synthesis mix: 2 μl ATP (Adenosine triphosphate, 75 mM), 2 μl GTP (Cytidin triphosphate, 75 mM), 2 μl GTP (Guanosin triphosphate, 75 mM), 2 μl UTP (Uridin triphosphate, 75 mM), 1.5 μl 5-(3-aminoallyl)-Uridin triphosphate and 2 μ, reaction buffer and 2 μl Enzyme mix (both provided with Ambion MegaScript Kit, Ambion, Cambridgeshire, United Kingdom).  
      This aRNA synthesis mix was incubated for 4 hours at 37° C. Remaining double strand DNA was digested by adding 1 μl Dnase I for 15 min at 37° C. aRNA was cleaned and concentrated with an RNeasy® Mini Kit column (Qiagen, Hilden Germany) and then concentrated to a final volume of 9 μl.  
      Fluorescent dye molecules were coupled to the reactive aminoallyl groups of the incorporated a 5-(3-aminoallyl)-Uridin triphosphate molecules. One aliquot of either Cy3™- or Cy5™-mono reactive dye (Amersham Biosciences, Buckinghamshire, United Kingdom) was diluted in 40 μl water free Dimethyl sulfonoxide. 10 μl, of one of the diluted Cy™ mono reactive dyes was added to each sample buffered in 100 mM Carbonate buffer (pH 9.00). Reaction was quenched after 1 hour by adding 10.4 μl Ethanol amine for 15 min at room temperature.  
      Unincorporated dye molecules were removed by ethanol precipitation. 2 μl Glycogen (Invitrogen, Basel, Switzerland) was added as carrier during precipitation. After precipitation aRNA pellet was washed with 80% ethanol, dried and resuspended in 50 μl 1× Fragmentation buffer (200 mM Tris(hydroxymethyl)aminomethane hydrochloride, 500 mM Potassium acetate, 150 mM Magnesium acetate). aRNA was fragmented for 35 min at 94° C. and placed on ice immediately. Fragmented aRNA was dissolved in 900 μl hybridization buffer.  
      For denaturation aRNA was incubated for 5 min at 98° C. and centrifuged for 30 sec at full speed in a microcentrifuge.  
      One CART-CHIP™ 300 (Millenium Biologix AG, Switzerland) was placed face down in a standard hybridization chamber. Hybridization solution containing the denatured and labeled aRNA sample was injected using a standard micropipet whereas Cy3™ and Cy5™ samples were hybridized together in one hybridization chamber (Millenium Biologix AG, Switzerland). The microarrays were incubated overnight at 42° C. in a PCR thermal cycler (TGradient, Whatman Biometra GmbH, Göttingen, Germany).  
      After incubation unspecific aRNA probe was washed away with 1×SSC, 0.1% SDS for 5 min at room temperature, followed by another wash step in 1×SSC, 0.1% SDS for 5 min and rinsed with 1×SSC without SDS for 1 minute to remove excessive SDS. 1×SSC was discarded. Remaining 1×SSC buffer on the slide surface was removed by centrifuge the slide for 2 min at 1500×g.  
      The dried CART-CHIP™ 300 were then scanned using an Affymetrix 418 microarray scanner.  
      Expression level raw data for every spot was obtained with ImageQuaNT (Molecular Dynamics). Raw data was normalized by dividing every expression value by total expression value of all spots for every sample and filtered by setting all values below the 25 percentile to the value of this 25 percentile to remove noise (25 percentile threshold).  
      For each sample (e.g. de-differentiated and re-differentiated chondorcytes) a list of all measured genes was generated. This so called gene expression profile was then used for subsequent analyses.  
      Further data analysis was performed using either hierarchical clustering with cluster.exe (written by Michael Eisen, Stanford University) or Self Organizing Maps (SOM), such as GeneCluster developed by Whitehead Institute (Massachusetts Institute of Technology, MIT). The settings of the software were optimized until a reasonable number of clusters resulted that were able to represent the comparison thoroughly. In the following example the parameters were as following:  
      Basic parameters: SOM rows 6; SOM col: 4; #epochs=3000; #seeds=1  
      Advanced parameters: initialization: random vectors; neighborhood: bubble; alpha 1=2; alpha f=0.005; sigma 1=3000; sigma f=2.  
       FIG. 1  shows a typical result from a SOM analysis with the above mentioned basic parameters, whereas  FIG. 2  shows an example of a graphical presentation of a cluster analysis and viewed by the software treeview.  
     Example 2  
     Quality Control and Human Cartilage Sample Classification  
      Useful to demonstrate how CART-CHIP™ 300 can be used to differentiate between diverse cell culture conditions, to distinguish different patients, to study the influence of 3D culture conditions and to serve as a quality control tool during any tissue engineering process.  
      Human chondrocytes isolated from 4 different donors were proliferated over one passage (P1) and then cultivated as high density pellets (0.5*10 6  cells) in 3D culture for 7 and 14 days. RNA samples were taken from proliferated as well as from 3D cultured cells resulting in totally 12 different samples as shown in  FIG. 8 . RNA isolated from this samples was shreddered in a QIAshredder (QIAGEN, Hilden, Germany), amplified, hybridized, washed and scanned as described in Example 1.  
      Data sets for all 12 samples were extracted and normalized as described in Example 1 to perform cluster and SOM analysis as noted below. Cluster analysis was performed using normalized data computed with GeneCluster.  
       FIG. 3  shows a picture of such a cluster analysis for all 12 samples (#1-#12) consisting of 20 clusters (c0-c19)  
      Every cluster represents a typical gene expression pattern for all 12 samples indicated by a point, starting from sample #1 on the left hand side to sample #12 on the right hand side in every cluster. For example cluster c0 represents the expression level of 104 genes in all 12 samples in a given range indicated by the lines located above and below the computed points.  
      Another example for gene expression levels that behave similar for different culture conditions and donors are depicted in clusters c3, c4, c9 and c10. Meaning that every subset of the three donor specific points #143, #4-#6, #7-#9, #10-#12 (see Tab V for detailed description) have gene clusters that behave similar in all analyzed samples.  
      An example of differently behaving genes is indicated in cluster c13, representing 10 genes that behave similar in donor #1 and #2 but show a different gene expression patterns for donors #3 and #4.  
      More detailed analyses are shown in  FIG. 4 ,  FIG. 5 , and  FIG. 6 . The clusters produced in these figures clearly demonstrate differences as well as similarities in cell behavior for either t0, t7 or t14 days, respectively.  
      Another software algorithm that can be applied for analysis of large amounts of data coming from gene microarrays is called hierarchical cluster analysis, whereas genes and/or different conditions with similar behavior in gene expression are clustered together. All hierarchical cluster analyses were performed using Cluster software described in Eisen et al. (1998) PNAS 95:14863) and displayed using treeview.exe developed by same author.  
       FIG. 7  shows such a cluster of selected genes for all 12 samples analyzed. Every square is representing one single gene expression value. Different intensity means different expression levels. Dark squares are representing samples without any significant change in gene expression compare to the other samples or patients. Bright squares are indicating samples in which genes are up- or down-regulated relative to other samples analyzed. A so called cluster of genes is a group of genes that behave similar from one donor to the other donors.  
      Not only genes but also samples can be clustered together. These clusters are called similarity dendrograms, shown in the top part of  FIG. 7 . These tree-like structures illustrates similarities in gene expression between different samples or donors. The closer a sample (#1 . . . #12) is located to another sample in this dendrogram the more similar gene pattern they have.  
      Interestingly to see is that the seven samples located at the right side of the dendrogram (samples #1, #2, #5, #7, #8, #10 and #11) are clustered together. This samples are representing t0 and t7 conditions as described above (illustrated in Tab V), whereas a cluster of 4 samples in the middle of the dendrogram (samples #3, #6, #9 and #12) are representing only t14 samples. This means a microarray of the current invention is able to distinguish between de-differentiated, proliferated samples (t0 and t7) and re-differentiated samples in a later stage (t14).  
      An outlier represents sample #4 located at the most left side of  FIG. 7 . which represents proliferated chondrocytes (t0) from donor 2 and could not clustered together with the remaining proliferated samples. Interestingly, this sample that it is not similar to all other proliferated samples (#1, #7 and #10) was impaired with its capacity to form cartilage tissue equivalents following expandation in 2D culture. The biochemical analysis revealed a lower amount of total collagen/DNA for this sample and immunohistochemisty with collagen II antibodies resulted in only weak staining for a collagen II.  
     Example 3  
     Aortic Fibroblasts vs. Chondrocytes  
      Example to differentiate between expanded chondrocytes and aortic fibroblasts cultivated over 14 days in 3D settings.  
      A human aortic fibroblast cell source was proliferated and brought to 3D culture. RNA was isolated after 14 days of culture. Expression data analysis was performed as described in previous Examples 1 and 2 using CART-CHIP™ 300 microarray.  
      A hierarchical cluster analysis was performed as described in example 2. Samples representing 3D culture after 14 days (t14) were included in said data analysis (samples #3, # 6, # 9 and #12, see Tab V).  
      The result of the described analysis can be seen in  FIG. 8 . The upper part of the figure shows a dendrogram as described in example 2. Aortic fibroblasts are not clustered together with human chondrocytes. The cluster shows a significantly different pattern compared to all other cultures.  
      Obviously a gene expression pattern of an aortic fibroblast cell source can be clearly separated from a gene expression pattern of human chondrocytes. A micorarray of the present invention is therefore not only able to study differences between different chondrocyte culture conditions but also to distinguish between cells isolated from different tissues.  
     Example 4  
     Arthritic Conditions vs. Healthy Conditions  
      Useful to distinguish between normal healthy chondrocyte behavior from cells resembling an arthritic phenotype. Interleukin-1β is known to play a central role in the inflammation and connective tissue destruction observed in both rheumatoid arthritis (RA) and osteoarthitis (OA). Stimulation of in vitro chondrocyte cultures with Interleukin-1β thus represents a simple experimental arthritis model.  
      The chondrocyte cell source from donor 4 (see Tab V) was proliferated over 3 passages and then cultivated as high-density pellet cultures (0.5*10 6  cells) for 16 hours and 7 days either in the absence or presence of Interleukin-1β (30 ng/mL). RNA was isolated from all samples, hybridized to CART-CHIP™ 300 and expression profiles were generated as described in Example 1.  
      A hierarchical cluster analysis was performed as described in Example 1 and the dendrogram and a selection of the representative gene clusters are shown in  FIG. 9 . This clearly shows that already a short stimulus of Interleukin-1β results in alteration of the chondrocyte phenotype with gene expression changes that can be distinguished from untreated normal chondrocyte cultures.  
      Appendix  
                       TABLE I                           Number of marker           Experiment and correlated gene   genes for each   experiment       expression analysis   experiment (analysis)   (analysis)                                            2D marker adult vs. fetal/infant   151   A       2D/3D adult vs. fetal/infant   96   B       3D marker failure   165   C       3D marker adult/fetal/infant   350   D       2D/3D marker adult   48   E       Time dependent failure marker   75   F       3D failure marker   41   G       Apoptosis related failure markers   30   H                  
 
     
       
         
           
               
             
               
                 TABLE V 
               
             
            
               
                   
               
               
                   
               
               
                 Sample Numbers (#) 
               
            
           
           
               
               
               
               
            
               
                   
                 Proliferation 
                 7 days in 3D culture 
                 14 days in 3D culture 
               
               
                   
                 (t0) 
                 (t7) 
                 (t14) 
               
               
                   
               
               
                 Donor 1 
                 #1 
                 #2 
                 #3 
               
               
                 Donor 2 
                 #4 
                 #5 
                 #6 
               
               
                 Donor 3 
                 #7 
                 #8 
                 #9 
               
               
                 Donor 4 
                  #10 
                  #11 
                  #12 
               
               
                   
               
            
           
         
       
     
      Table IV shows the results of a bioinformatic analysis of gene expression profiles of the 467 cartilage specific marker genes.  
                                                                      Clus-                                   Accesion   ter   SOM Description   H4 P2 3D   failure pool   H5 P1 2D   H4 P2 2D   H4 P1 2D   H5 P1 3D               AA283693   2   2D/3D   0.620146952   0.563012608   0.543651567   0.467462855   0.502962907   0.971864748       AA845156   2   2D/3D   1.822887384   1.974062517   1.793508407   2.056234156   2.134502741   2.109619189       R52548   22   2D/3D   42.15305285   24.91632836   36.03204148   38.20234471   38.12886555   36.73272848       T67128   4   2D/3D   0.114907897   0.165223543   0.175143838   0.230515232   0.111518632   0.115949318       AA845015   8   2D/3D   5.152985898   5.163366386   4.600617698   5.490400618   5.613170246   5.081143315       AA937895   8   2D/3D   2.841572293   2.035680155   2.574530008   3.532376413   2.904240444   2.88987266       AA844998   15   2D/3D   9.597114182   13.63928438   14.05976269   12.99542024   10.56590581   11.73420854       AA844518   16   2D/3D   34.95251239   32.56636092   35.29612046   39.31305252   38.10953207   32.87879549       AA894557   16   2D/3D   56.23673424   43.61031652   36.30704958   58.47875881   59.70042623   48.33981986       AA872001   20   2D/3D   81.81389212   56.44454367   38.30704958   89.11888026   82.12028621   54.71499842       H09590   1   2D/3D   0.538758103   0.606028834   0.923090371   0.610970303   0.582446119   0.488768664       AA888278   11   2D/3D failure vs.   0.872435407   1.541743481   1.320841185   1.418331235   1.44054309   1.142574932               cartilage       AA490855   11   2D/3D failure vs.   5.256355042   3.982907846   3.740473099   5.019900855   5.072810576   4.193769533               cartilage       H05820   2   2D/3D failure vs.   0.326771454   0.298568027   0.723972051   0.373421152   0.291228161   0.42426813               cartilage       N57766   3   2D/3D failure vs.   1.244580679   1.430645893   1.237128762   1.435760892   1.621084111   1.793426963               cartilage       AA873885   3   2D/3D failure vs.   2.389977721   1.402865274   1.960300849   2.6421261823   1.174416144   2.132327967               cartilage       AA878880   3   2D/3D failure vs.   1.975516413   0.706271534   2.235200516   2.852909562   2.341957596   2.325462095               cartilage       R54818   6   2D/3D failure vs.   1.433222617   1.408764041   1.026467036   1.653245959   1.555280568   1.313068482               cartilage       AA458830   23   2D/3D failure vs.   6.225911646   7.131102869   6.449691069   7.181223742   7.435528281   8.273231774               cartilage       W37884   11   2D/3D failure vs.   3.42542852   3.434948923   2.822843216   3.920408386   4.031440051   2.924316539               cartilage       N63192   11   2D/3D failure vs.   2.016416698   1.880341817   1.955128455   2.604284112   2.555986174   1.868963757               cartilage       R55789   11   2D/3D failure vs.   3.050734207   2.916359387   2.459270284   3.086180697   2.768681643   2.87080332               cartilage       R56871   14   2D/3D failure vs.   8.05304356   6.763218112   9.202602512   9.315742803   9.656817425   6.703182113               cartilage       AA448659   15   2D/3D failure vs.   4.086696472   7.404785733   3.70432959   4.624265635   4.780951569   6.12097414               cartilage       AA235388   11   2D/3D failure vs.   4.793907209   5.590616298   6.430749719   6.119406496   5.787215762   5.448903875               cartilage       W37769   18   2D/3D failure (adult)   9.666153573   9.041797201   10.48060069   9.18235887   9.779057041   9.301866835       AA421701   18   2D/3D failure (adult)   6.39851543   6.944977864   6.787000717   7.805796465   8.044148563   6.091762141       NB1029   22   2D/3D failure (adult)   11.25612666   7.536201205   9.791822816   10.26909779   11.09189885   11.24056884       AA644128   22   2D/3D failure (adult)   8.87008786   8.634238881   8.980680688   10.62210945   11.07634385   8.151499526       N26536   23   2D/3D failure (adult)   1.127588919   0.934050212   1.276862277   1.339090617   1.722862887   1.543708239       AA890663   23   2D/3D failure (adult)   7.502466565   6.586516448   8.707110583   7.582524442   6.272458865   6.92700675       AA405987   17   2D/3D failure (adult)   8.007848934   7.398131991   7.180024565   9.644484085   9.329435489   7.017060375       AA888182   4   2D/3D failure (adult)   1.159709394   0.997393508   1.761866087   1.609558388   3.076463138   1.864862168       H09730   23   2D/3D failure (adult)   6.016598561   4.38097919   4.512218706   5.75467456   5.708409303   4.568792922       AA285155   22   2D/3D failure (adult)   3.911163963   4.375731694   4.103404996   3.976701687   3.566916248   4.846020855       AA873351   1   2D/3D failure (adult)   0.715716774   0.448961688   1.143094938   1.086305702   0.6643881   0.576661574       H12320   1   2D/3D failure (adult)   0.561973556   2.57723008   0.516528265   0.364969035   0.426769958   1.536698149       AA856558   1   2D/3D failure (adult)   2.083875002   0.734906688   0.98056715   1.17363108   0.340692619   1.146139751       R43581   2   2D/3D failure (adult)   2.106534421   0.601495831   2.998509555   1.885349164   1.78560062   1.913887067       AA633768   2   2D/3D failure (adult)   0.641660349   0.394897957   0.989161585   0.80349418   0.676931906   0.471526265       AA496880   2   2D/3D failure (adult)   0.819001028   0.446244605   1.078973938   0.96946743   1.001567705   0.612915544       AA625632   4   2D/3D failure (adult)   1.493144759   0.535655307   2.320048835   2.145585116   1.461514048   1.208574247       R40850   3   2D/3D failure (adult)   0.682308084   0.595008017   1.325881758   1.168113039   0.576613768   0.894548353       AA486072   9   2D/3D failure (adult)   2.033091331   6.009395549   1.397241773   1.306335917   1.667598386   4.321686833       N80129   4   2D/3D failure (adult)   0.351536161   0.860381999   1.012366932   0.336466005   0.442915198   0.530986466       T67270   5   2D/3D failure (adult)   1.963529863   1.201108371   3.314347202   3.108011309   2.159811403   1.603756559       AA776304   6   2D/3D failure (adult)   0.862462885   0.468437574   1.000125084   1.050963409   0.840547206   0.712318096       AA64743   6   2D/3D failure (adult)   1.419135024   0.972417085   1.72467226   1.536612587   1.441826888   1.267977482       AA663983   7   2D/3D failure (adult)   0.992237246   0.562108496   2.11407474   1.505738573   1.504937007   1.363338621       AA634008   8   2D/3D failure (adult)   1.633316095   0.546444823   1.670198138   2.668127971   1.579545189   1.258079751       AA683050   8   2D/3D failure (adult)   1.604349305   0.609305829   2.869733356   2.49429974   1.663928936   1.276592262       AA775874   3   2D/3D failure (adult)   1.787634446   0.639410548   1.3560157   1.263003994   1.234797131   2.355664777       AA029934   19   2D/3D failure (adult)   2.356636943   1.558846586   2.787420472   2.743130089   2.330296295   2.141155369       AA872397   21   3D adult vs. fetal   19.50233862   17.51369555   30.1499536   23.26630505   24.52945535   17.84127943       AA428195   3   3D adult vs. fetal   7.071920614   5.267967863   6.138557001   7.23507727   7.48271081   5.35099208       AA478724   3   3D adult vs. fetal   5.248364716   3.470387154   4.172040349   5.052705318   5.168529149   6.015264941       T40541   2   3D adult vs. fetal   3.238889946   12.31479553   2.521870024   1.484626573   2.463205882   6.610009772       N33214   2   3D adult vs. fetal   2.65473448   2.961197481   1.727113613   1.517196864   1.863643598   5.009412045       W69399   2   3D adult vs. fetal   2.836334543   2.418647835   2.384859773   1.271557098   2.218662035   4.984390107       H85454   2   3D adult vs. fetal   2.580688668   1.88582991   1.388608005   1.244844015   1.534483051   3.983108173       T71284   2   3D adult vs. fetal   1.986879018   2.475258573   0.744183047   0.768307025   0.765085426   4.427870608       N95418   2   3D adult vs. fetal   3.21585913   1.583715156   1.572090076   1.152454683   1.55045145   4.188689886       AA430675   18   3D adult vs. fetal   20.40011187   13.5115773   23.35924913   19.03556124   22.70504494   16.6788312       AA682851   21   3D adult vs. fetal   25.44236224   17.10952218   33.76684841   28.22129217   26.86052023   24.00871315       AA427433   3   3D adult vs. fetal   6.682930727   4.737477088   5.985736157   7.06879228   7.217223212   6.130314027       AA100296   21   3D adult vs. fetal   16.73518464   14.16222538   25.17519653   21.63898477   21.16013508   17.85039728       AA070997   20   3D adult vs. fetal   21.13329064   16.5080222   30.63855114   26.1438582   26.27182686   19.46457951       R27585   20   3D adult vs. fetal   18.95998765   13.51396253   26.82697423   21.45047839   21.44832633   16.96978325       N71628   20   3D adult vs. fetal   18.60838994   16.33393806   26.68120529   21.6113028   21.33877846   16.98487296       AA484566   20   3D adult vs. fetal   16.06992797   10.85633998   18.70563179   17.27026288   17.70073984   14.07448353       AA043228   20   3D adult vs. fetal   12.81312824   9.803849829   17.97620829   14.19332756   14.28559054   10.85546077       AA478273   19   3D adult vs. fetal   52.06929197   32.17017287   36.30704958   47.93432102   43.94005516   45.65265571       H05618   2   3D adult vs. fetal   2.632906988   4.642300363   1.284880525   0.973906438   1.326880261   4.570563698       AA405562   5   3D adult vs. fetal   6.993441389   4.782974749   5.219581264   6.611843236   6.447811984   5.853372531       AA147043   5   3D adult vs. fetal   5.26712976   4.856265473   8.499381882   5.561464269   5.30607401   5.35955551       AA035384   5   3D adult vs. fetal   6.401728539   4.12666021   7.578771481   6.904866564   7.128981272   5.672655498       R60150   5   3D adult vs. fetal   4.215197474   4.38442636   4.860194724   4.973592031   5.301613877   4.146462455       N64051   5   3D adult vs. fetal   5.426681636   4.928078432   5.608870527   5.622962025   5.371210998   4.561721051       AA405748   5   3D adult vs. fetal   5.368605864   3.19002151   4.353339926   4.106442793   5.477050315   4.525369392       AA461110   5   3D adult vs. fetal   3.575328316   2.604624915   4.130714878   3.915938843   4.131312686   3.482794563       AA845167   5   3D adult vs. fetal   3.78578459   3.609211434   4.618092536   4.244750176   4.779380405   3.825719327       AA443118   5   3D adult vs. fetal   4.314381512   6.09876062   6.276759492   5.303887624   5.568448187   5.169715811       N92319   3   3D adult vs. fetal   4.572213322   3.033513374   3.354505947   3.067302105   3.243795499   6.319683552       AA187148   5   3D adult vs. fetal   5.511820527   5.984833947   9.561458451   7.538771775   7.330918349   4.996228711       AA253413   3   3D adult vs. fetal   5.753953945   4.708057923   5.13409191   8.221909098   6.216223439   4.722726065       AA045701   4   3D adult vs. fetal   7.244740869   7.004200569   9.55974171   8.437552392   9.263082719   6.552005827       AA164562   4   3D adult vs. fetal   7.627425507   5.216496788   8.699986728   7.923881699   9.508233607   6.844029007       AA486357   4   3D adult vs. fetal   6.41575883   6.975113261   10.40229719   7.96745072   8.41742583   6.451127762       AA180742   4   3D adult vs. fetal   5.52121367   6.234552553   8.103930847   6.983167569   6.946238241   6.26480073       AA454743   4   3D adult vs. fetal   7.511488312   9.369158012   11.34696747   9.562551962   9.456937824   7.36978659       AA437226   4   3D adult vs. fetal   7.112744497   8.791653264   10.80511143   8.266289799   8.507470188   7.034847567       AA458849   3   3D adult vs. fetal   5.469328544   3.66373147   4.903306694   4.827076947   5.660206542   4.514629804       AA604891   3   3D adult vs. fetal   5.258178068   8.251213126   6.90322093   6.369912634   5.676523521   7.288981598       AA609655   18   3D adult vs. fetal   21.33429032   19.08081783   30.01089002   23.9982204   25.31681248   20.18144049       AA599158   6   3D adult vs. fetal   5.73905777   4.765585417   8.346245119   6.423634441   6.61080494   5.063695516       AA052932   2   3D adult vs. fetal   4.160294487   2.183896002   2.762899749   2.096861401   2.192248117   5.413063915       AA769328   2   3D adult vs. fetal   3.49664111   3.795346147   3.543244396   3.202155462   1.664435653   4.680897198       AA129537   6   3D adult vs. fetal   5.490099148   4.033511964   6.753224678   5.270505052   6.104594281   4.571138177       AA486209   2   3D adult vs. fetal   2.951127838   2.622458107   2.396628552   1.876778497   2.154038566   4.999645899       H39016   2   3D adult vs. fetal   2.815824978   1.826966689   1.671944771   1.253970839   1.643279353   4.632563322       AA464217   2   3D adult vs. fetal   2.270265016   2.131631711   1.520659008   1.064618518   1.329400245   4.21418601       T95053   2   3D adult vs. fetal   2.688707692   5.33382498   1.794683232   1.531765242   1.715814755   5.3630072       AA454640   2   3D adult vs. fetal   2.102886248   1.537570373   1.346779699   1.127849141   1.214174754   4.183264486       AA448400   2   3D adult vs. fetal   3.306242137   2.235006202   1.747624063   0.631226114   1.568082834   8.474436694       H13691   19   3D adult vs. fetal   54.00052945   38.06027395   36.30704958   53.01474929   49.86794621   50.27373427       AA132086   2   3D adult vs. fetal   3.337097743   3.257550359   2.293125381   1.567514408   1.604751828   5.862999872       AA488073   2   3D adult vs. fetal   4.351033023   4.04194944   1.452692862   3.511334664   4.118886207   5.708773871       N40945   2   3D adult vs. fetal   3.447096842   2.154588097   2.501683281   2.52085183   2.297385171   4.762374759       R55705   2   3D adult vs. fetal   2.909308613   1.851341621   1.432688968   1.212749384   1.638105054   4.556458468       H50114   2   3D adult vs. fetal   3.301736569   1.908387922   1.705840938   1.407414465   1.781048668   4.589212608       AA452841   2   3D adult vs. fetal   3.981943611   1.231373773   1.0248198   0.963983432   1.620834153   4.505595396       W73790   2   3D adult vs. fetal   3.914888537   1.713927735   1.679258731   1.608715372   2.253084809   4.790266687       N30302   17   3D adult vs. fetal   16.236426   14.33825503   20.17733457   16.72882972   18.60385984   15.82011639       AA291556   2   3D adult vs. fetal   2.858389118   6.164431067   2.312941111   1.624968319   1.959001687   5.460005926       AA598510   2   3D adult vs. fetal   2.698323673   2.653383075   1.709197068   1.448745687   1.852274765   5.166038663       AA453787   2   3D adult vs. fetal   2.702589425   1.620596967   1.717336506   1.499079329   1.522616002   4.378038549       H05655   2   3D adult vs. fetal   3.107515398   2.521892845   1.311805573   1.314199679   1.633779252   6.087070828       AA419177   18   3D adult vs. fetal   23.89742962   15.45485589   25.88217585   24.47790114   28.20825412   20.47309058       AA458807   18   3D adult vs. fetal   22.02916221   16.65443357   25.45874485   22.43485375   25.67182927   18.88009347       AA293218   18   3D adult vs. fetal   19.79668624   15.40443015   27.23128953   22.13209523   21.07504567   18.14351405       W44860   17   3D adult vs. fetal   12.7340951   10.689677   18.07307043   13.89427187   15.92917087   12.73205002       AA629862   17   3D adult vs. fetal   14.59679193   11.53376895   17.21229223   15.59989608   16.64541758   14.01615062       AA447674   2   3D adult vs. fetal   2.534264127   2.150154393   1.427789252   1.000328271   1.423442229   4.137781882       T52484   2   3D adult vs. fetal   3.236398407   3.026511181   1.838920999   1.617002106   1.759710423   4.94920807       AA496810   2   3D adult vs. fetal   2.803422463   1.595989735   1.728921373   1.455020012   1.72732132   4.877833042       AA486233   2   3D adult vs. fetal   1.105294528   3.612629566   2.016514163   1.407057353   1.146012765   4.325105426       AA079775   2   3D adult vs. fetal   3.60412957   2.70567137   3.009055265   2.126182568   2.517322618   5.903186938       W73889   2   3D adult vs. fetal   3.174704174   4.228312305   3.583500944   3.632704149   3.233152734   6.073296967       R50337   2   3D adult vs. fetal   2.252389181   1.301117771   1.696068083   1.246881305   1.59150746   3.84378734       R55046   2   3D adult vs. fetal   4.804068001   3.914420759   4.479334588   5.310431247   5.155223566   4.292901695       R46821   2   3D adult vs. fetal   4.481781503   3.60517107   3.194315863   3.736730328   4.059831528   3.62674894       R87763   2   3D adult vs. fetal   4.349371289   1.738958085   2.016576353   1.564246255   2.167157105   4.929744779       H89583   2   3D adult vs. fetal   3.472010108   3.168123065   3.070758833   1.876335035   2.529708287   5.529732134       R56040   2   3D adult vs. fetal   2.875034039   12.02198937   3.636042926   3.134295566   2.862500876   5.657443535       AA922705   2   3D adult vs. fetal   2.467900912   5.753848696   2.364224943   1.826235564   1.990935084   5.56199203       AA487571   2   3D adult vs. fetal   2.392095741   2.030046874   1.8982167   1.167380172   1.445241279   3.891969777       AA402440   2   3D adult vs. fetal   3.04018443   4.537574439   2.816667278   2.313436127   2.567871927   6.461198799       H29521   2   3D adult vs. fetal   3.458079561   2.089088468   1.722877258   1.456445529   1.929668192   5.361928045       AA490911   15   3D adult vs. fetal   42.67030392   26.52859985   36.28250422   40.39466602   41.60811969   34.97057694       AA486082   13   3D adult vs. fetal   10.41727609   8.937884327   15.05040142   10.82056372   11.95282018   8.842288293       AA678065   17   3D adult vs. fetal   14.12093138   11.54070062   20.86168208   18.075708   17.83849384   13.73636722       R43509   16   3D adult vs. fetal   11.02563598   9.509595949   16.74133733   14.13918278   13.48520494   10.63815358       N57653   16   3D adult vs. fetal   10.4123206   10.44346779   14.67805884   11.74181676   11.74500654   11.69858601       AA670955   16   3D adult vs. fetal   9.121610176   8.547640672   16.37945858   12.25345162   12.21640163   9.258251347       R14692   2   3D adult vs. fetal   0.757198559   0.588063899   0.752811699   0.852655502   1.447179728   0.869563322       AA488979   15   3D adult vs. fetal   33.66640515   23.40785359   36.22684956   35.83780071   36.1199539   29.2581877       AA443630   2   3D adult vs. fetal   3.179366912   1.141747364   1.766810452   1.118906727   1.631839103   4.49413229       AA027840   14   3D adult vs. fetal   30.77584038   23.58752538   32.62815407   33.05944103   33.60205655   25.63272733       AA458830   14   3D adult vs. fetal   25.58379208   26.39848683   28.15722788   25.87408416   27.40987838   24.06147297       AA459015   13   3D adult vs. fetal   10.03176884   8.709006371   16.03563015   13.10174715   14.03043114   9.884872553       AA074446   13   3D adult vs. fetal   11.85807943   9.620046415   13.82726113   12.87569434   14.04256345   11.73842466       AA027042   13   3D adult vs. fetal   12.73491005   9.459896144   16.51983878   13.49994947   14.93224524   12.43468402       AA629923   13   3D adult vs. fetal   11.09427639   6.096835847   11.63735402   10.20004947   10.48961254   9.430135362       AA460830   15   3D adult vs. fetal   43.46763026   25.89585549   36.23321738   37.95742356   39.87905887   37.77709716       AA454218   0   3D adult vs. fetal   4.470293684   3.093239424   4.937529756   4.688122666   5.072419486   3.72227458       AA046523   6   3D adult vs. fetal   6.155805263   4.388837355   6.934532229   5.949254753   6.883321967   5.158132106       R51346   0   3D adult vs. fetal   3.230511245   3.062418161   3.787520043   3.783285032   3.826003563   2.512202334       AA029964   0   3D adult vs. fetal   3.855847135   4.014123191   5.758888572   4.613362037   4.007280855   3.687657081       AA489219   0   3D adult vs. fetal   3.274998157   2.361717684   3.918052974   3.243068103   3.469099389   3.176498463       AA043133   0   3D adult vs. fetal   3.039842745   3.471121389   5.203529353   4.221352079   4.259092185   3.419382811       AA812973   17   3D adult vs. fetal   15.77248197   10.74549128   18.85394679   18.86663157   16.77333141   12.70379222       AA453471   0   3D adult vs. fetal   3.957168294   3.038926799   2.87937811   4.188692511   4.453834895   3.457945931       AA284693   17   3D adult vs. fetal   14.4127364   9.497839898   13.96717518   12.75585136   13.63554527   14.18625183       N90281   0   3D adult vs. fetal   2.889108898   3.065073021   3.825043424   3.772722446   3.897897917   2.916608408       AA629542   0   3D adult vs. fetal   3.339124505   3.20278143   4.020290022   4.367848138   4.203343071   3.045430554       AA679345   0   3D adult vs. fetal   4.041914565   3.40189177   5.334580535   4.767347184   5.052052168   3.629044952       H37774   2   3D adult vs. fetal   2.582136051   5.751409544   0.826130431   0.749198653   0.950396052   4.924705249       T97181   2   3D adult vs. fetal   2.848144946   1.762304259   1.780931817   1.662977237   1.930489483   4.429079742       AA454879   2   3D adult vs. fetal   2.588814818   1.895822968   0.788622997   0.683939522   0.868426864   4.58445785       AA147640   0   3D adult vs. fetal   3.111174182   3.689139309   3.781524296   4.096860799   3.959011774   2.732271995       AA767429   9   3D adult vs. fetal   7.054093591   5.363580152   7.988105567   6.839604508   7.370692262   7.981701609       AA490991   9   3D adult vs. fetal   10.98757317   7.790729538   12.35710247   9.885681916   11.00435577   8.898305631       AA422058   17   3D adult vs. fetal   14.71408525   14.84685565   24.49176062   19.94632048   19.29291385   14.68049409       N66208   9   3D adult vs. fetal   8.123797741   6.177962556   10.33188119   7.894725917   8.580898297   6.881934203       AA630776   9   3D adult vs. fetal   9.138119273   5.83233298   11.41953939   9.003209098   9.590093969   8.070647196       AA827287   9   3D adult vs. fetal   8.482649348   7.482270307   8.891746969   9.19984268   9.976725792   8.422494842       AA488084   9   3D adult vs. fetal   7.649134152   14.97955716   13.41766713   10.42093881   9.071703388   8.874743158       R89715   13   3D adult vs. fetal   9.750099751   17.8705502   18.03616416   13.82091334   12.99735343   14.90421282       AA490501   9   3D adult vs. fetal   7.944415444   7.85975819   11.06033198   9.284097489   8.992468434   7.63431741       N32199   9   3D adult vs. fetal   9.67828656   7.893329723   9.916311099   9.837236279   11.23987563   9.150031823       AA434404   9   3D adult vs. fetal   6.86730459   8.426674472   9.577263322   8.037236674   8.096867575   6.953180054       N93688   8   3D adult vs. fetal   5.038274076   10.94187624   9.036823839   7.358456237   7.155250657   6.336977302       AA292676   7   3D adult vs. fetal   11.09420423   9.867844396   9.548860422   11.52855409   12.53274971   9.824831453       AA464417   7   3D adult vs. fetal   10.40978662   9.6398749   11.95762629   12.77651551   12.8767479   10.07601153       AA442092   7   3D adult vs. fetal   9.965987122   7.370289148   8.883701613   9.441691799   9.667688043   8.214697052       AA442092   7   3D adult vs. fetal   9.965987122   7.370289148   8.883701613   9.441691799   9.667688043   8.214697052       AA026644   7   3D adult vs. fetal   7.769602383   6.638214592   5.137119018   3.572083072   4.086915401   15.10517784       AA481464   9   3D adult vs. fetal   7.356616049   9.394727631   11.96871523   9.624488789   9.697819022   7.196159079       T68859   12   3D adult vs. fetal   7.102239287   6.063011273   10.14342977   8.316121437   8.90719198   7.165656319       AA699560   13   3D adult vs. fetal   9.978421471   9.37295778   14.75863286   16.42689227   14.26471651   12.73562928       AA705069   13   3D adult vs. fetal   8.512749427   10.51595378   13.19983017   9.999000301   10.13397175   9.694546633       AA457739   13   3D adult vs. fetal   13.76296688   10.27635708   15.4062123   12.76652957   14.0993498   10.82804945       H99843   13   3D adult vs. fetal   11.42545366   9.860489604   11.08358091   10.09631162   9.902204667   10.73353077       AA399410   13   3D adult vs. fetal   11.49268913   9.868304848   16.50988414   14.49672712   13.23658995   10.91245055       AA443039   9   3D adult vs. fetal   8.661615681   5.473553572   10.7592073   8.268347579   9.771073691   7.541136242       AA164440   11   3D adult vs. fetal   17.82989233   11.87877628   20.35395821   18.48267711   19.46815644   14.70024382       AA446453   11   3D adult vs. fetal   16.55396138   13.81116994   16.50294651   19.54048563   21.22775124   16.02767268       AA280692   11   3D adult vs. fetal   17.40750225   16.18669704   19.04935817   19.71347085   20.1673057   16.35083848       AA031514   11   3D adult vs. fetal   20.58635757   17.50162009   21.03844179   22.39941193   21.41470955   18.33395551       R33154   11   3D adult vs. fetal   15.80929053   13.23696939   13.37191076   14.52813826   14.95312607   16.05415087       AA487452   11   3D adult vs. fetal   13.71327193   19.41866534   12.61831663   12.077349   12.74498437   18.11228999       AA400329   9   3D adult vs. fetal   9.471410325   8.139099224   9.945496246   10.13837609   11.074429   7.250594994       AA454668   13   3D adult vs. fetal   9.047350014   7.5254115   12.60652925   10.81932261   10.34313891   8.424799177       AA486393   23   3D adult vs. failure   0.682030067   6.724440565   0.800620868   0.562299276   0.603381368   1.639697615       R62541   23   3D adult vs. failure   0.402099116   2.047232943   0.476534818   0.419746076   0.364790601   0.848514165       AA171613   23   3D adult vs. failure   0.833916304   6.428013019   1.049489533   0.831618144   1.061543998   2.831110499       AA235706   0   3D adult vs. failure   103.2862145   45.18459033   36.30704958   66.22096461   76.19336098   53.68696068       AA668527   23   3D adult vs. failure   1.360133945   6.805032154   1.071215078   0.97529683   1.06310668   3.121521767       T64144   23   3D adult vs. failure   0.476915725   7.417782102   0.746262622   0.455935776   0.588907616   1.573719963       R14080   23   3D adult vs. failure   0.442812641   7.906110384   0.627950807   0.498614946   0.372466179   1.218619609       AA609599   23   3D adult vs. failure   0.359352461   7.143369152   0.569305177   0.366281857   0.341488403   1.345297086       AA489201   23   3D adult vs. failure   0.746247673   7.761267038   0.569230512   0.568039398   0.51445757   1.823393758       R08876   23   3D adult vs. failure   0.881300108   6.204438487   1.346594192   1.127374945   1.303511748   1.365140688       H46425   23   3D adult vs. failure   0.446119129   7.549550263   0.42423628   0.384125705   0.304256579   1.948954368       R56149   20   3D adult vs. failure   3.946739485   2.221889535   2.687396748   2.29349791   2.994436137   6.00466275       AA454619   20   3D adult vs. failure   4.670053967   2.36899368   3.285602699   2.811568095   3.530544823   6.168078813       H15445   20   3D adult vs. failure   3.638147066   2.706447087   1.458885277   1.457265129   2.021283714   6.315381377       AA705225   23   3D adult vs. failure   1.386836786   6.429576897   1.83711377   1.34483513   1.150500104   2.597429853       AA191488   2   3D adult vs. failure   2.174224052   45.10144344   2.069008838   1.631870516   1.77653582   16.32613468       N64862   14   3D adult vs. failure   0.650596095   12.10838017   1.065306504   0.665682947   0.614517248   3.167265877       R45413   7   3D adult vs. failure   0.716578413   26.61952355   1.065046051   0.917779985   0.773891766   6.012284228       R77293   7   3D adult vs. failure   4.173270014   18.87787869   6.098081459   6.158463331   5.192489399   6.808820493       AA436187   6   3D adult vs. failure   7.73084558   34.64438609   9.740301386   7.582520052   8.233604749   18.4559413       AA676470   5   3D adult vs. failure   23.09461759   12.79749882   19.67261192   18.83195374   25.31283192   19.5015243       AA443634   23   3D adult vs. failure   0.483674724   6.507766668   0.834546801   0.602237179   0.563061629   1.705323839       AA664180   4   3D adult vs. failure   77.28842805   43.66418943   36.30704958   68.26659959   73.01397471   54.50243711       W58658   20   3D adult vs. failure   3.738957043   1.157647486   1.625298607   0.567045634   1.362263758   5.290498458       H54023   2   3D adult vs. failure   2.518425666   76.64764128   1.455767277   1.89558391   2.244043815   23.68020326       H73724   11   3D adult vs. failure   0.794565272   15.90487626   1.124742532   0.660242857   0.688814595   4.296719254       T70031   2   3D adult vs. failure   2.006155548   48.92472369   2.789740103   2.01477414   1.746671803   15.29766287       AA481758   0   3D adult vs. failure   123.6211644   61.20693911   36.30704958   92.35920856   102.0498037   54.85301063       AA521431   0   3D adult vs. failure   125.2413904   56.77999766   36.30704958   93.6827224   100.8362156   54.85301063       AA446103   23   3D adult vs. failure   0.338624303   7.652704012   0.571045963   0.349989394   0.406883851   1.434663101       N92646   5   3D adult vs. failure   30.42865911   14.89709888   29.90172172   26.59339088   28.56672605   26.04183434       AA453789   18   3D adult vs. failure   2.044150452   8.495958017   2.328026154   2.572167528   2.212159729   3.457082607       AA425299   18   3D adult vs. failure   7.887770078   3.918963058   10.38387994   7.660089272   8.794304531   7.329938113       AA868929   18   3D adult vs. failure   1.98091121   9.937368538   2.013089465   1.471073914   1.420828724   5.418655459       R60019   18   3D adult vs. failure   2.116044289   8.525464296   1.047037018   0.928042539   1.082727505   5.235772895       AA857343   18   3D adult vs. failure   1.650037418   6.297808737   2.426871459   1.704623126   1.741966813   3.845429741       AA481438   18   3D adult vs. failure   2.573933498   8.391310909   2.445625176   1.893217317   1.76341218   6.043734557       AA399674   18   3D adult vs. failure   2.130783417   8.164685436   3.388970859   2.354039954   2.279993542   4.872785488       T98887   18   3D adult vs. failure   1.100740148   8.756420509   0.936100822   1.100317922   0.803584044   3.484794007       AA678404   18   3D adult vs. failure   1.601598727   7.015197466   1.932547665   1.820935977   1.80852363   3.313612045       H15747   20   3D adult vs. failure   3.703368787   1.841236561   1.31983711   0.468266355   1.579189807   6.384201738       H16958   16   3D adult vs. failure   4.911652574   2.505777016   6.153238607   3.258278988   9.421877751   2.976025283       AA936783   14   3D adult vs. failure   1.342031028   13.57092172   1.952974192   1.334954568   1.22240126   1.341547085       AA884709   18   3D adult vs. failure   2.262402026   7.467527538   3.158379807   2.652355075   2.663007264   4.51372877       H24688   18   3D adult vs. failure   1.395480263   7.059371875   2.232669864   1.921932565   1.963742023   4.014560107       AA884403   18   3D adult vs. failure   2.032526808   8.508216003   2.408869338   2.267218044   2.281983003   4.451591143       AA404619   19   3D adult vs. failure   0.721996555   11.04039228   0.849034927   0.475083665   0.645471218   2.92914746       AA598611   19   3D adult vs. failure   0.473381741   9.658226818   0.485642718   0.382776293   0.327497562   3.321739       H72875   19   3D adult vs. failure   1.047528607   9.965593016   1.7514717648   0.96145731   0.718146053   3.174877726       H63361   19   3D adult vs. failure   0.444705701   8.608586298   0.460617918   0.347764767   0.327526489   2.835780951       R39221   19   3D adult vs. failure   0.063457623   8.80369405   0.916404688   0.588441567   0.583832961   2.21717036       R02348   19   3D adult vs. failure   0.380957115   8.723284539   0.689843744   0.430680409   0.314907846   1.628641297       R51835   19   3D adult vs. failure   0.319821038   3.205091988   0.499133148   0.341800718   0.398772973   0.702868877       R33031   19   3D adult vs. failure   0.309199103   9.871901353   0.870719013   0.498982302   0.446815901   1.737947999       AA412053   18   3D adult vs. failure   2.173462159   8.658749377   1.560525939   2.335301865   1.60001113   3.312435004       AA001897   11   3D adult vs. failure   0.707399619   18.56199185   1.276833043   0.839394093   0.698445289   3.44857855       W81191   18   3D adult vs. failure   2.035130827   9.564497621   1.470028438   1.278460058   1.466681545   5.442558928       AA430552   16   3D adult vs. failure   6.704743363   3.912744879   7.308427625   6.182487835   6.715639324   5.892347254       AA594130   20   3D adult vs. failure   4.197684546   1.950948544   3.399747789   2.792799271   3.308148608   6.573561928       N92864   20   3D adult vs. failure   3.964889092   1.12030555   1.255047434   1.028875207   1.597688392   6.129597747       AA457123   20   3D adult vs. failure   3.825435082   1.372454684   1.737581719   1.805411888   2.242579776   5.776097858       R43320   19   3D adult vs. failure   0.534524185   10.13392358   0.307540341   0.334762409   0.297154033   3.300796986       AA670430   20   3D adult vs. failure   6.033965543   2.411089781   6.490413776   5.227507565   6.00488788   4.958892131       H85068   11   3D adult vs. failure   0.932240825   19.08305372   1.062851862   1.323524085   0.843208322   3.42676519       AA458785   11   3D adult vs. failure   1.024544481   14.76220711   1.673515465   1.016303139   1.117923958   3.660946499       AA485871   11   3D adult vs. failure   1.240425485   16.32124036   1.819664978   1.051475658   1.243832378   4.885742561       T39411   14   3D adult vs. failure   0.683416906   14.1280482   0.827762784   0.665895165   0.776309726   3.661139864       R00855   20   3D adult vs. failure   4.116868708   2.545089683   2.768201659   2.658761885   2.788015121   6.090967819       H98666   16   3D adult vs. failure   7.496261053   3.423039388   3.917399183   3.208047795   4.021338011   7.436138134       H72028   14   3D adult vs. failure   1.122387248   10.87725529   1.107269289   0.894651185   0.693097551   5.481280912       AA679177   14   3D adult vs. failure   0.813601986   11.3980019   0.884729818   0.738756843   0.624486657   3.353148409       N21578   14   3D adult vs. failure   0.493867961   12.22018711   0.630068988   0.332239214   0.452874405   3.48979683       AA007419   14   3D adult vs. failure   0.640548282   10.87290796   0.573707373   0.426598512   0.390973266   5.000691355       T49657   14   3D adult vs. failure   1.478438588   12.2454622   1.66172702   1.085853458   1.158150176   4.728340076       N38959   14   3D adult vs. failure   0.96668518   12.21521754   1.222240919   0.390241965   1.036587317   3.877992922       R51912   14   3D adult vs. failure   0.931685691   13.32666832   0.554065456   0.584470901   0.545379233   4.794685202       H90415   14   3D adult vs. failure   1.027933738   14.40938868   1.543560847   1.015015198   0.913466897   3.697172386       H41489   1   2D/3D adult vs. fetal   4.027389786   2.097422239   1.726349793   1.413362414   2.223616983   7.753646206       H16456   1   2D/3D adult vs. fetal   4.015386257   7.010456083   2.974647228   2.512493381   3.106940998   8.704678862       W45415   1   2D/3D adult vs. fetal   5.612363827   1.065216881   2.691703268   2.027056421   2.568973782   6.615995267       AA447751   1   2D/3D adult vs. fetal   3.707303465   4.780241073   2.223590687   2.437972778   2.631458156   7.623606101       AA487486   1   2D/3D adult vs. fetal   4.21336596   4.045765801   3.015283294   2.286202019   2.768948605   8.048478397       R56604   1   2D/3D adult vs. fetal   4.165186307   3.846779908   2.973815371   2.369929936   3.1509812   7.458099126       T65772   1   2D/3D adult vs. fetal   4.487863913   2.538286605   2.643015763   2.462140712   3.0062686448   0.741762884       H15085   1   2D/3D adult vs. fetal   4.532070688   2.552606255   1.874728576   1.797928653   2.249323538   7.10815384       R61295   1   2D/3D adult vs. fetal   5.7522625   3.64542013   2.945120833   2.564363772   2.826103944   7.68061273       T61256   1   2D/3D adult vs. fetal   5.260364   1.641968589   1.819566112   1.625856706   2.432049565   6.879367501       AA405731   0   2D/3D adult vs. fetal   2.090454022   34.42033666   1.664515998   1.304288382   1.293105338   10.6339838       T71879   1   2D/3D adult vs. fetal   4.193732889   4.210242665   1.985666897   1.946658119   2.195662508   8.143137484       R59927   1   2D/3D adult vs. fetal   4.826208077   4.383351971   1.602344146   1.52557434   2.192290627   7.925477266       AA496780   0   2D/3D adult vs. fetal   1.514704846   26.44459843   1.135159078   0.912692615   1.001084793   10.48006188       AA176888   0   2D/3D adult vs. fetal   0.883613375   16.90708911   0.969072645   0.759370466   0.781878516   6.758214974       AA436163   0   2D/3D adult vs. fetal   1.145286483   19.39007997   0.91032868   0.79908737   1.062812492   7.470804263       AA428778   0   2D/3D adult vs. fetal   2.705936237   23.81604244   1.650059764   1.291875843   1.526455491   10.05785097       AA463225   0   2D/3D adult vs. fetal   1.805467836   22.97689102   1.545226155   1.416083191   1.289595725   9.717200851       AA485426   0   2D/3D adult vs. fetal   1.389325695   25.67139954   1.27039293   1.069742478   0.998457685   9.858144828       W47485   0   2D/3D adult vs. fetal   1.745341956   27.39946025   1.287668441   1.305011386   1.416492719   11.04790889       H84982   0   2D/3D adult vs. fetal   1.533032723   28.30131199   1.047589817   1.549633971   1.23686481   9.828318446       AA504615   1   2D/3D adult vs. fetal   4.050257188   3.625356301   2.199703889   1.941384876   2.75118289   7.641495993       H94487   0   2D/3D adult vs. fetal   1.645750443   22.47903843   3.594820042   1.028845936   1.681686149   8.286429124       AA448959   0   2D/3D adult vs. fetal   3.124990125   4.592355812   3.899374735   3.173734392   3.451263958   6.793092921       AA070358   2   2D/3D adult vs. fetal   8.012667475   1.564102379   1.883538283   1.584996753   2.642393389   9.48000834       AA453401   1   2D/3D adult vs. fetal   5.692934592   1.528593629   1.905652383   1.904350696   2.206453207   6.672888766       N66737   14   2D/3D adult vs. fetal   24.90073908   0.393334078   1.095420779   0.556298638   0.923759381   3.442649218       AA666180   9   2D/3D adult vs. fetal   2.115740805   12.05725753   2.14570443   1.488045473   1.870792054   5.34373246       AA857131   9   2D/3D adult vs. fetal   0.152032673   0.207460704   0.268997471   0.210685319   0.132367162   0.357440795       AA479102   9   2D/3D adult vs. fetal   2.295197382   25.01632531   3.681214605   3.425928026   3.02851033   8.49611205       AA456077   7   2D/3D adult vs. fetal   7.731170292   4.509291885   4.761631135   3.922605205   4.875603995   11.60167555       R87497   7   2D/3D adult vs. fetal   7.553857203   4.518297667   3.006217917   1.067794468   4.393661357   12.60889243       AA718910   7   2D/3D adult vs. fetal   7.12414318   3.548431737   3.928552441   3.557814726   4.383101911   10.82120865       AA406269   7   2D/3D adult vs. fetal   5.887777587   6.493700059   3.376570613   2.359008775   3.447900579   11.90806873       N74623   4   2D/3D adult vs. fetal   0.738692411   0.885632206   0.507569185   0.423927496   0.405894222   1.326758097       H99364   6   2D/3D adult vs. fetal   4.654446832   3.967945222   3.173155342   2.53020258   2.826020625   9.199591084       AA447684   6   2D/3D adult vs. fetal   3.976408745   14.43816072   4.763738151   2.471882235   2.732135077   8.763139341       AA282301   6   2D/3D adult vs. fetal   5.741753046   2.638926534   3.525955597   3.116535142   3.442563042   8.08592064       H99588   5   2D/3D adult vs. fetal   1.455459706   29.42112357   0.93818073   0.705663294   0.91408074   9.587415089       N53512   5   2D/3D adult vs. fetal   1.885890833   27.43755749   1.437831064   1.488193294   1.489514434   11.66412529       AA683321   5   2D/3D adult vs. fetal   1.133236265   27.22345134   1.394968386   0.968365361   0.71972912   8.309498154       AA608557   5   2D/3D adult vs. fetal   2.577273943   29.04646478   2.807387159   2.029202014   1.932689792   8.478935818       AA757764   2   2D/3D adult vs. fetal   5.690914196   3.357895765   3.881101888   2.752079821   2.955536228   9.043391314       AA406064   2   2D/3D adult vs. fetal   6.957108219   3.855068049   3.928274978   3.830306615   5.143577569   9.248530935       N54598   20   2D/3D adult vs. fetal   6.411864475   40.45864114   12.32476105   8.740060641   8.361428458   11.67500348       AA481988   7   2D/3D adult vs. fetal   6.029716769   7.619281816   3.801345873   3.597022308   3.988124217   12.08244483       N62394   3   2D Marker adult vs. fetal   2.436813979   3.687865805   1.411911686   4.045770491   0.844150341   2.656973499       N20148   3   2D Marker adult vs. fetal   3.44995341   2.759942512   3.751806548   3.90090485   3.142378907   4.04494813       AA496678   3   2D Marker adult vs. fetal   7.001100843   4.03568563   5.297409583   3.722383923   5.160647823   10.19230959       AA400973   3   2D Marker adult vs. fetal   2.89346803   4.47076738   4.470210209   3.193478491   3.257043268   4.020015252       AA497027   3   2D Marker adult vs. fetal   1.802250483   0.578162102   3.922092662   2.407065102   1.286762868   2.157496735       N64508   3   2D Marker adult vs. fetal   2.115795984   3.276856168   1.90235047   5.314258483   2.788331395   3.540796101       AA033564   3   2D Marker adult vs. fetal   5.316788651   2.720044947   3.133883202   2.683871596   3.532284326   8.161295359       AA446108   3   2D Marker adult vs. fetal   2.078301935   2.211041944   3.792759943   3.324684095   3.375402585   2.756568814       AA159577   3   2D Marker adult vs. fetal   4.746090464   4.989070689   4.287813007   3.593768792   3.816425244   7.786959581       R36958   3   2D Marker adult vs. fetal   4.749539144   3.400588787   5.624793806   5.234422192   5.286395351   4.078616944       AA829508   3   2D Marker adult vs. fetal   1.920163507   0.7909906   3.923258324   3.457491381   1.13174848   1.710017559       AA482067   3   2D Marker adult vs. fetal   6.194625221   4.238881966   3.254611387   3.315340662   3.879401032   8.171254871       AA669314   3   2D Marker adult vs. fetal   6.809538669   4.133050912   5.329740351   3.780076886   4.424821889   8.385883026       AA775241   3   2D Marker adult vs. fetal   6.014247582   4.222679024   5.909433903   4.651935893   4.529769385   7.71821785       R73584   3   2D Marker adult vs. fetal   2.788220682   5.744755801   4.174172878   3.110031036   2.840523285   3.830886431       H28984   5   2D Marker adult vs. fetal   2.892697648   3.831327794   4.337837094   4.289702271   3.643114467   3.029043454       R44202   3   2D Marker adult vs. fetal   3.843410664   2.636358787   3.786734961   3.415590384   3.473443896   3.189323952       W70051   4   2D Marker adult vs. fetal   0.460743655   0.435637611   0.599111691   0.634647941   0.684508861   0.518398472       AA401972   5   2D Marker adult vs. fetal   4.694309329   5.237266898   4.803264368   6.3957827   6.187809857   5.273916388       AA236164   5   2D Marker adult vs. fetal   4.489190329   5.999132856   6.533218517   5.791985355   5.205993927   5.089326434       R22412   5   2D Marker adult vs. fetal   3.322360737   8.924589287   5.257612264   4.2528688   4.433039078   4.372991798       AA424804   5   2D Marker adult vs. fetal   6.72316462   4.945597359   8.844706058   7.100383442   7.690736998   5.712216735       AA669443   5   2D Marker adult vs. fetal   3.473141273   5.642074949   5.804136792   4.780513481   4.819190027   3.624913174       N69689   2   2D Marker adult vs. fetal   1.47268426   1.500609747   1.577772215   1.63158512   1.673578671   1.20775425       H24316   4   2D Marker adult vs. fetal   4.097759837   4.571809686   6.670594998   5.519559327   5.302346746   4.332423617       AA074224   3   2D Marker adult vs. fetal   2.916040437   42.17908573   3.996084321   3.421263628   2.977614141   15.7136877       R36571   4   2D Marker adult vs. fetal   1.22088245   0.697979245   1.246173941   1.106008283   1.434340053   1.173334441       AA058465   4   2D Marker adult vs. fetal   5.113683679   10.12779251   5.977620638   5.183053806   5.656909478   5.941479667       AA633811   4   2D Marker adult vs. fetal   5.047585747   3.541329987   5.859700035   4.917562173   6.033089317   4.6898963101       AA457155   4   2D Marker adult vs. fetal   4.59919155   2.634463051   5.207796008   3.444600254   3.888657977   4.888009989       AA459104   4   2D Marker adult vs. fetal   4.790849026   2.49717776   4.875933559   4.052637562   4.532277466   3.791654155       R40212   4   2D Marker adult vs. fetal   5.695606523   6.536605083   5.50014925   4.878309221   5.754823259   8.404118354       AA086476   4   2D Marker adult vs. fetal   3.826188364   2.338234619   4.05845178   3.573649092   3.942866534   3.486560771       AA683310   5   2D Marker adult vs. fetal   4.988875925   0.427535974   6.884450204   7.059542516   6.212911071   5.167664215       AA455640   1   2D Marker adult vs. fetal   2.29694825   2.246509212   2.490940902   2.65444359   2.528889751   2.024350751       AA496878   1   2D Marker adult vs. fetal   2.329206672   2.464809211   2.708923499   2.591526171   2.820757906   2.528269298       AA085749   1   2D Marker adult vs. fetal   1.134352576   1.145219423   0.919694908   1.213892433   1.191952306   1.148805945       AA425755   1   2D Marker adult vs. fetal   1.256026253   1.42629856   1.456101082   1.538376189   1.521167266   0.95599115       N52350   1   2D Marker adult vs. fetal   2.159542225   2.651354597   2.427419475   2.256040758   2.484151334   2.217863077       AA630104   1   2D Marker adult vs. fetal   1.898590687   1.850429013   2.323118997   2.265340283   2.543886171   1.567701571       AA454854   1   2D Marker adult vs. fetal   1.85505455   1.874219048   2.289079847   2.076882204   1.775527487   1.600396337       W73406   2   2D Marker adult vs. fetal   1.145861817   1.688694122   1.394720158   1.5150059   1.348266115   1.257230067       R12802   1   2D Marker adult vs. fetal   0.754036384   0.458756191   2.051238468   1.123879939   0.741672703   6.651716983       AA465355   2   2D Marker adult vs. fetal   2.427318967   1.234497382   1.382975298   2.820706627   1.758025333   2.561266549       AA829383   1   2D Marker adult vs. fetal   0.627874118   0.361600207   0.637447262   0.608221718   0.513908343   0.712979229       AA629189   1   2D Marker adult vs. fetal   3.384460323   3.907165525   3.933343191   4.23836901   3.468746044   3.982435808       AA430512   0   2D Marker adult vs. fetal   2.239479445   2.637756736   3.81876171   3.36943129   3.404540535   2.413395122       AA456439   0   2D Marker adult vs. fetal   3.838221727   2.779439139   4.396947289   4.325871481   4.445703612   3.414286314       H27864   0   2D Marker adult vs. fetal   4.083067399   2.588260193   3.247167731   3.682989494   4.052835461   3.076145132       AA644657   0   2D Marker adult vs. fetal   2.749565753   1.949529953   3.282718575   2.77240442   2.835386395   2.393138312       R40460   0   2D Marker adult vs. fetal   2.617741285   2.77044995   2.345768887   2.530348937   2.639132034   2.129569504       W96058   1   2D Marker adult vs. fetal   0.551793053   0.719935768   0.823172955   0.716948884   0.647054285   0.511406835       T72202   2   2D Marker adult vs. fetal   1.372133229   0.654777758   1.354496565   1.335828319   1.299148537   1.132910683       AA598794   3   2D Marker adult vs. fetal   0.857314271   0.38760124   4.80929041   4.449090919   3.2675415   0.950844105       AA599178   3   2D Marker adult vs. fetal   1.987500842   1.076238697   3.616532831   3.470498129   2.405945118   1.70047288       R88247   3   2D Marker adult vs. fetal   9.295559476   4.145659051   3.807036517   3.247695912   4.811556959   18.08933692       T98812   3   2D Marker adult vs. fetal   3.206007578   0.45582699   7.36008566   3.762388177   4.629393762   2.262352025       AA454858   3   2D Marker adult vs. fetal   7.687530164   3.130374276   3.770230181   3.357268094   3.90840295   11.3903899       N67048   2   2D Marker adult vs. fetal   0.182901012   0.36769394   0.297974786   0.30807128   0.27564065   0.333601513       AA776875   2   2D Marker adult vs. fetal   1.088783309   0.850650279   1.034076531   1.167561655   0.998364163   0.903409454       H51117   2   2D Marker adult vs. fetal   0.537932542   0.687368102   0.574022043   0.730299571   0.683526033   0.743361636       N36174   5   2D Marker adult vs. fetal   6.089061165   4.481415857   5.540191589   5.623320599   6.588429457   4.496005766       AA777187   2   2D Marker adult vs. fetal   1.523155704   1.077976801   2.060524963   1.946327352   2.299471554   1.87592009       R09581   8   2D Marker adult vs. fetal   5.890519553   3.784033955   5.786261707   4.733579692   4.524489662   5.169772778       R16849   2   2D Marker adult vs. fetal   1.23979305   1.31739538   1.989966506   0.663751034   1.594500153   1.415959831       AA884167   2   2D Marker adult vs. fetal   1.217420137   0.76877689   0.848143007   1.070264972   1.102669725   1.097445419       AA136983   2   2D Marker adult vs. fetal   1.988298813   2.638681789   2.540675832   2.650969074   2.517626988   1.836297875       AA488622   2   2D Marker adult vs. fetal   1.834368714   2.664699416   2.916200392   2.716178444   2.280636291   1.670136989       AA699427   2   2D Marker adult vs. fetal   1.74393914   2.642651634   2.705734803   2.790851259   2.361629453   1.978094745       AA490459   3   2D Marker adult vs. fetal   6.36833046   5.671979456   3.680374244   4.936768605   4.884731709   9.255585154       AA626787   2   2D Marker adult vs. fetal   1.688259404   2.164212705   2.115946285   1.795197631   2.352850319   2.121107168       N62179   15   2D Marker adult vs. fetal   35.50826346   19.11160591   33.5269723   27.57938931   35.32468308   29.21398971       N27190   16   2D Marker adult vs. fetal   9.750993225   7.530891576   12.75918957   11.05916225   12.03535219   8.653835849       AA441895   16   2D Marker adult vs. fetal   7.177282564   5.235719613   7.385439482   7.014086953   8.126515643   6.246092446       AA463924   16   2D Marker adult vs. fetal   10.41970608   7.964616858   12.55072628   11.42665371   12.31290239   9.520621452       N78843   16   2D Marker adult vs. fetal   7.66464154   5.43569693   7.61347718   7.608367958   8.883600715   5.827918931       AA629719   16   2D Marker adult vs. fetal   14.07113881   8.07158282   15.07029688   13.18842124   13.89479922   12.78716552       AA404755   16   2D Marker adult vs. fetal   10.9079267   19.01587747   12.27393806   10.03671065   11.20438161   12.29601389       AA459351   7   2D Marker adult vs. fetal   3.30738816   5.313693876   7.122668851   3.529883953   3.671031365   4.776266565       AA488346   16   2D Marker adult vs. fetal   9.488691926   7.542950447   11.21693205   9.805337766   10.46454533   9.180810589       AA427899   17   2D Marker adult vs. fetal   13.3365169   9.041851128   16.97946166   12.44206045   15.16820039   11.53880939       AA453813   15   2D Marker adult vs. fetal   23.53014662   18.55051373   26.13232552   24.07754444   27.05702114   19.41686645       AA397824   15   2D Marker adult vs. fetal   28.08351962   20.62648968   27.02787266   26.29257303   30.85067131   19.80045327       AA633901   15   2D Marker adult vs. fetal   34.77984918   23.78442887   36.12250762   35.61488434   41.66703684   30.60345109       AA181334   15   2D Marker adult vs. fetal   27.28075277   18.98160489   31.18437988   27.23129085   29.89868199   24.05531978       AA292410   15   2D Marker adult vs. fetal   31.30959843   29.13821092   33.60796008   35.98378599   38.0810897   27.93817331       AA253434   15   2D Marker adult vs. fetal   27.28836746   24.48751872   26.021072   29.51377393   31.81897761   24.38068282       AA455058   16   2D Marker adult vs. fetal   12.54762444   10.90299499   12.04192978   13.96133558   14.93518352   10.87358414       R55188   21   2D Marker adult vs. fetal   31.12874965   12.81904882   26.29604395   18.72935308   27.16189634   19.64403686       AA465723   0   2D Marker adult vs. fetal   0.626099905   0.519829853   0.965056345   0.801834491   0.723063592   0.645073541       N49856   23   2D Marker adult vs. fetal   58.25496123   43.81206927   36.30704958   58.88107815   63.73545411   51.53609212       AA455272   23   2D Marker adult vs. fetal   78.7226545   57.95745288   36.30704958   70.83386123   84.60136833   54.29877782       AA459292   23   2D Marker adult vs. fetal   84.98013907   54.55237528   38.30704958   78.81031291   85.94545998   54.78113735       AA878561   23   2D Marker adult vs. fetal   91.89023489   70.24733836   36.30704958   85.95771786   103.4556246   54.83812392       AA772068   23   2D Marker adult vs. fetal   100.7022767   60.91386332   36.30704958   95.8034517   85.63154687   54.85301063       N78821   17   2D Marker adult vs. fetal   18.18260113   8.900969331   17.95968118   14.88223883   16.81550288   14.041252       AA291490   21   2D Marker adult vs. fetal   16.15200019   12.2589771   19.64085664   16.98965403   18.88134711   13.99432924       N46828   17   2D Marker adult vs. fetal   16.54096886   9.830784212   17.53351276   13.86763897   16.838674726   15.02548971       AA150487   21   2D Marker adult vs. fetal   15.19512412   13.80042191   17.95609277   14.5094675   17.55056275   15.10253571       AA282537   21   2D Marker adult vs. fetal   17.24244099   13.64870386   21.82923027   18.53902602   18.34433042   17.95537212       AA707922   21   2D Marker adult vs. fetal   29.52423481   19.17248102   30.42814242   29.09439668   27.854176   25.96068749       AA443638   20   2D Marker adult vs. fetal   15.1000594   15.15348782   17.88558853   15.7697112   18.94151907   14.61633105       W73892   19   2D Marker adult vs. fetal   53.62002013   39.02380655   36.30704958   56.67674826   53.82921611   46.25990911       N70734   19   2D Marker adult vs. fetal   50.1395528   37.01986341   36.30704958   48.48335304   47.57551429   45.25023946       H57136   13   2D Marker adult vs. fetal   10.27199646   9.124487788   12.5238108   9.80585148   11.12532179   10.90999216       AA700414   29   2D Marker adult vs. fetal   72.81271834   37.61546462   36.30704958   68.67343991   62.25292659   54.4411949       W65461   7   2D Marker adult vs. fetal   5.476839239   5.067442981   5.426887725   6.079126118   6.4014634348   5.626362584       AAA36584   15   2D Marker adult vs. fetal   23.02941313   15.24754038   25.73740515   22.61556954   22.7981159   19.91483801       AA029042   9   2D Marker adult vs. fetal   8.022234644   7.311596005   8.895662277   9.479217739   9.605485934   7.544837461       AA427725   9   2D Marker adult vs. fetal   12.69294864   7.655536142   13.67326812   4.261155334   12.4290971   12.81248352       N51280   9   2D Marker adult vs. fetal   6.632333815   5.874595041   7.385166272   7.520608106   7.785748506   5.963530521       AA281347   8   2D Marker adult vs. fetal   5.089780613   4.640487592   4.9415594344   5.45251025   6.288204512   4.490066004       AA402960   8   2D Marker adult vs. fetal   4.965150621   4.50182924   6.596223821   2.242032928   4.880356598   4.839655851       N98485   9   2D Marker adult vs. fetal   7.125405927   6.544129951   7.7721864   7.556753899   8.479066808   6.411666818       AA490209   7   2D Marker adult vs. fetal   5.803194729   5.024903   4.546705412   6.046321655   6.500609214   4.920009122       W61361   9   2D Marker adult vs. fetal   7.384750912   7.335298925   11.10935518   8.8100027   9.421761776   6.881790584       N51018   7   2D Marker adult vs. fetal   5.488100802   38.59262312   10.6998308   6.032584586   5.216849242   17.32422488       AA455281   7   2D Marker adult vs. fetal   6.998082782   9.317433876   8.495460011   7.352959068   7.938571107   8.110370507       W69471   7   2D Marker adult vs. fetal   7.215022797   8.148565459   8.197656911   5.32000885   4.956859017   13.55485747       AA486321   7   2D Marker adult vs. fetal   6.616843406   3.688421667   10.1148224   5.538922361   5.404776773   6.062776617       AA458982   7   2D Marker adult vs. fetal   6.961286958   6.280975266   6.441469774   7.072922475   7.646598009   6.402438243       AA442095   7   2D Marker adult vs. fetal   10.53368867   6.969251828   9.035104314   8.530732052   8.715924875   7.353607298       N99003   7   2D Marker adult vs. fetal   7.777346527   5.123406597   7.271360835   7.694076299   8.015056524   6.947984869       AA609284   7   2D Marker adult vs. fetal   5.203527631   6.725228727   5.962252816   5.392872692   5.486984016   6.647332466       AA195036   12   2D Marker adult vs. fetal   4.739031812   5.630223489   6.168419327   3.952137125   6.299183956   4.511434885       AA478268   5   2D Marker adult vs. fetal   4.587623085   4.319668521   6.07446736   5.321094819   5.705783899   4.273131909       AA608583   13   2D Marker adult vs. fetal   8.710213419   6.633631914   10.89267006   9.897803204   10.76405611   7.594364628       AA480435   13   2D Marker adult vs. fetal   10.66603107   6.878393398   11.70702321   9.862860466   10.62920807   10.9035389       AA505046   13   2D Marker adult vs. fetal   6.175908969   7.774740452   10.84632581   7.657584613   7.632328468   5.432221512       AA487893   13   2D Marker adult vs. fetal   6.28913391   7.086629819   10.38317033   8.186637919   8.677781639   6.646527414       AA292226   12   2D Marker adult vs. fetal   6.978311765   9.498379167   9.999757753   8.245173408   8.45533271   7.944255354       H87106   9   2D Marker adult vs. fetal   8.511773166   8.306766068   8.639728411   8.688270747   9.817268292   7.409517924       W96450   12   2D Marker adult vs. fetal   6.333304604   6.11454874   10.93854816   8.295025536   8.501848685   6.781838425       N33331   13   2D Marker adult vs. fetal   12.01197758   9.753283045   14.0583162   10.92456794   13.45540044   19.89513482       AA405800   12   2D Marker adult vs. fetal   6.121396479   5.223320588   7.721500548   7.718106658   6.462547598   5.753462094       T51539   12   2D Marker adult vs. fetal   6.489900146   6.570558251   9.469029997   8.820264288   9.194419114   5.883142749       N59764   12   2D Marker adult vs. fetal   7.600540659   4.756724822   8.39191538   7.279906425   8.135204655   6.251234677       AA521346   11   2D Marker adult vs. fetal   18.0187463   11.98813991   20.04539147   17.66437285   19.72793582   15.44668566       AA428551   11   2D Marker adult vs. fetal   11.81379838   9.132344514   13.57703545   12.536771   14.26115078   11.3197892       AA489383   11   2D Marker adult vs. fetal   10.07196453   6.40710185   10.63654538   9.115267349   9.345806882   8.56845107       AA490172   11   2D Marker adult vs. fetal   8.374766147   1.415297485   17.38713256   12.09049918   8.290346893   6.184780432       AA504477   12   2D Marker adult vs. fetal   5.417891216   3.85972235   6.090353431   5.209247321   5.829042051   4.969815506                                                         Accesion   H1 V2 t0   H1 V2 t2d   H1 V2 t4d   H2 P1 3D   H2 P1 2D   H3 F P4 t0d   H3 F P4 t2d   H3 F P4 t7d               AA283693   3.60E−02   7.00E−02   0.322383183   0.447391374   0.257739798   0.143513725   0.213829818   5.224212686       AA845156   1.28342518   1.863140939   1.384248489   1.041642418   1.859400083   1.332668422   1.018759388   5.129921131       R52548   24.22599845   23.2864338   18.34158303   25.57074708   22.01795031   27.50011776   26.82422658   0.455256887       T67128   0.104460037   3.43E−02   1.158151225   0.122945522   0.194757907   0.263409159   0.266372201   15.92056233       AA845015   5.971265034   6.563146942   3.845471328   4.218022136   6.266907187   4.459497525   3.113814791   23.77610407       AA937895   0.452860231   0.364695951   1.498136505   1.34894881   1.524476699   1.004544129   0.332861119   23.03713043       AA844998   11.26686885   9.619376872   4.163473688   8.673344954   10.804701   11.6741356   8.48774821   0.687222458       AA844518   48.83476799   54.52879325   40.56584843   54.89200718   49.39056409   42.93573187   47.44855774   6.960856259       AA894557   49.93664852   44.42230199   49.4951755   65.92881949   60.33602671   39.11417155   46.47247102   1.451948868       AA872001   54.20005466   64.75076884   65.42329615   62.45668946   67.24659793   53.73423923   63.31563058   1.313345062       H09590   0.653737608   0.390597399   1.521400852   0.559410558   0.287251416   0.132045049   5.76E−02   9.888630279       AA888278   4.209025695   6.432026011   1.564621676   2.03670347   3.658833325   5.897415558   5.585056007   4.869119818       AA490855   7.139496238   7.480509788   2.402406359   2.11287386   3.291155604   4.163992221   4.626992228   4.040992653       H05820   0.250153138   0.2218924   0.884601448   0.443767203   0.853117504   0.577197042   0.227069034   8.569006814       N57766   0.161049809   1.029762957   0.860155011   1.160409378   0.696379763   1.945055757   1.505490705   18.21699443       AA873885   0.576137629   0.747123686   1.364947624   0.484042151   0.351548888   2.300286033   1.86211811   31.75585388       AA878880   0.214554665   0.751859199   1.555155015   0.580037834   0.413262131   0.487069801   0.493477276   17.65353573       R54818   0.646985459   0.409947934   0.199952191   0.325728665   0.57824012   0.478785614   0.148018732   5.693624857       AA458830   10.09192142   11.58519602   4.33841323   6.048695063   5.824480196   13.79679438   10.66742276   10.18586889       W37884   2.917746898   4.089689479   1.262910319   1.511275797   1.980865808   5.197874299   6.453757844   5.811360752       N63192   3.165162737   4.106218227   1.558423149   1.5903832   2.110133253   5.489587577   6.303860434   5.163746261       R55789   5.839714648   7.877144785   2.228165523   3.701220707   3.164509514   3.916307321   3.178401276   3.905915697       R56871   8.541201281   9.565561925   5.095438247   5.327868943   7.13865299   8.798222752   9.999533942   1.720941975       AA448659   6.40985601   8.062945347   2.837008591   2.740282482   3.524896196   8.26644815   9.131700385   6.724601997       AA235388   5.495213871   7.128779744   1.810297945   3.365604514   4.797167927   5.188848193   3.719920825   3.55603538       W37769   7.944164954   9.806778833   2.976028459   4.717147657   6.792587093   10.44168598   10.83208914   8.961263558       AA421701   8.998110252   11.20923244   3.344552795   4.634853853   5.630305124   9.009982779   7.214242967   8.676253864       NB1029   12.53804212   11.46996906   3.601513562   9.079241362   7.051304119   8.667406893   7.19394857   6.694763747       AA644128   11.11148757   10.68939675   3.391349693   5.57092524   5.21033759   8.326957952   9.801034583   8.395745244       N26536   12.17742976   6.963934008   2.083996724   2.539664871   3.338342813   6.091759705   5.481627936   4.663694738       AA890663   13.06099681   8.389833107   0.735677843   3.645361823   5.830896283   10.14275452   7.73405205   8.622524146       AA405987   10.80261435   12.021725   2.747874952   3.870971862   6.423478204   9.717748938   10.38278845   8.452850767       AA888182   1.015717155   1.005037796   10.31208553   1.080891115   0.620362547   0.742540888   0.217171056   0.963761651       H09730   11.63478455   9.263731897   2.426058114   3.698744788   6.251078878   11.24128172   10.27180594   8.218265778       AA285155   12.85800077   9.831563278   3.688749895   5.365619531   5.184975167   7.402395869   7.379800537   6.209664883       AA873351   0.470607591   0.631814307   7.979382806   0.79673277   0.500876851   0.326537695   0.408343693   0.899376594       H12320   0.282438829   1.169093316   7.275998896   1.197723988   0.103345305   0.205188914   0.219791049   0.757004021       AA856558   0.397739468   0.400975764   8.510141689   1.29264139   0.857904175   0.471466936   0.271477848   0.790926029       R43581   0.381477112   0.761735816   6.064087362   1.931094763   0.818248288   0.397440561   0.456001183   0.419248288       AA633768   0.760761379   0.497766033   5.606806863   0.633870185   0.393352577   0.351794529   0.601192682   1.14487198       AA496880   0.493894518   0.572564726   6.056796145   0.653312475   0.514470842   0.502539979   0.501308175   1.212402498       AA625632   0.842550697   0.850281232   10.68487135   1.661720166   0.695569959   0.498447008   0.274386994   1.69499       R40850   0.394670064   0.363742341   4.967702878   1.362523305   0.380406218   0.141276114   0.169003657   0.619590829       AA486072   1.063850678   3.668838298   12.43527006   3.538640747   0.712197565   1.714315274   1.299858757   2.072066795       N80129   0.10312387   0.42006458   10.27394074   0.364964803   0.173871475   3.53E−02   9.69E−02   1.445787697       T67270   0.706906482   1.1288523   9.031533244   1.747487433   1.676363321   0.971529338   1.02792629   1.159935914       AA776304   1.05308668   1.608943664   7.115065643   1.060601139   1.428674622   1.215371395   1.026193827   2.050698416       AA64743   0.720584255   1.207641114   6.709648148   1.123344154   0.916034946   0.548390406   0.6724641   1.948529432       AA663983   2.040575612   0.913188385   5.16313531   1.007867654   1.323649464   2.135989003   8.32E−02   3.811870062       AA634008   1.359339921   1.036497507   13.34177496   1.724576212   0.853238694   1.024786523   1.068524094   1.956193928       AA683050   1.013489887   1.038528355   15.81952721   1.58843476   0.496740614   0.558453728   0.815020862   1.866191062       AA775874   0.246852476   0.424685862   5.143824512   0.974500161   0.529437509   0.290250898   0.38554521   1.21495112       AA029934   10.39261667   6.173829216   3.256015168   2.937213126   3.76308931   6.716135076   4.994432581   4.353783816       AA872397   46.00745138   39.17088563   36.04470776   60.24320179   47.48372041   40.84667624   48.51971658   25.61671577       AA428195   5.50376757   5.85445104   2.298660486   2.154530299   3.416182085   4.515305124   4.564251077   3.09255509       AA478724   2.441015531   4.011719078   1.703691212   1.339633614   2.575865932   1.573760187   1.247433101   2.275822402       T40541   0.275153908   0.545092967   3.902618974   0.963575617   9.51E−02   8.50E−02   3.92E−02   1.06319634       N33214   0.13076449   0.257442727   1.248745094   1.214456176   0.147732522   0.479395409   5.23E−02   1.778833698       W69399   0.345223646   0.364082141   1.11200994   0.651631382   0.156264888   4.35E−02   5.26E−02   1.090858575       H85454   0.177423187   0.164239157   0.25952362   0.278813589   0.207718277   0.192428939   0.293284362   0.980664216       T71284   3.25E−02   4.43E−02   4.34E−03   0.114965169   0.174872171   0.141660059   0.315197363   0.637643567       N95418   7.17E−02   4.56E−02   0.17695605   0.230927882   2.96E−02   3.19E−02   1.92E−02   0.409050075       AA430675   29.3586379   28.49610318   30.86231132   32.28482203   44.49536923   33.27001036   32.81546787   20.72524354       AA682851   46.90243645   42.6704861   49.46272263   54.93185512   60.11034283   37.00532862   37.62408166   23.34221611       AA427433   4.590770914   6.491096293   2.119161449   2.512849593   3.622778825   5.405043355   5.599238453   5.427644127       AA100296   39.44423876   56.23942898   41.49374024   50.80500421   43.55754452   57.54473062   53.89058071   27.20955224       AA070997   44.50541019   42.47813824   34.76574485   44.39487164   50.26187697   33.36357052   29.205598   27.82261502       R27585   35.33261308   33.39411146   29.7220802   41.54702124   41.6509187   34.23348926   26.42844313   25.01170127       N71628   29.62587768   25.73280775   27.314568   42.44280692   32.44686223   28.50781759   31.40872062   17.11695396       AA484566   36.70082765   34.8718366       38.3684724   32.25023119   37.7985424   98.44938631   22.42777135       AA043228   35.07501988   35.26683595   39.99311042   38.42578454   28.64861089   33.09819082   42.01213318   20.20000016       AA478273   24.39470164   24.77474479   23.18365645   21.88455412   23.61056895   34.11709903   37.1720164   22.29749728       H05618   0.287634172   0.126355776   0.103328665   0.53496355   0.113769783   0.103142991   0.103572238   0.411960124       AA405562   13.78665714   15.04195781   4.06566807   10.07318456   10.12150087   9.869201884   10.11315283   9.063379829       AA147043   10.06577793   10.38848924   8.480460276   9.593900583   10.3223246   9.358593384   9.251590954   7.969342929       AA035384   12.04070004   12.92252006   15.89036927   11.03937435   13.98924616   10.56026075   7.850369977   8.464122084       R60150   9.633059103   11.34413022   3.55905762   5.14548369   8.554543073   8.219606806   10.11952936   7.877309364       N64051   20.20297   17.93454359   6.059875542   10.65425592   11.69250488   14.02690246   13.40703529   11.39979162       AA405748   11.19414229   11.22331463   8.725898139   10.22004433   9.939101581   9.624639345   6.736761694   7.207953341       AA461110   11.42516439   11.54464774   11.22768955   11.5865327   9.335135467   1.599312321   1.604370138   0.742502214       AA845167   15.24112672   15.33748563   10.34294903   10.49210118   9.895195623   11.54052428   13.14844812   9.845307435       AA443118   15.71033269   12.21523831   7.066371438   9.278769927   12.62454607   12.73679044   11.89501447   11.26385022       N92319   0.68013854   0.462694318   0.441158367   0.601101086   0.511427071   0.795108656   0.113909679   1.02641734       AA187148   11.23551547   10.38626391   7.898742343   8.98515106   8.886781354   9.776711665   9.067245371   5.447187287       AA253413   5.375893098   6.14287559   2.014829497   2.303548324   3.272998057   3.697482133   3.356931434   3.421688731       AA045701   16.07206256   16.18438115   15.35025693   11.75303107   17.53371006   21.28041445   23.57251468   18.29553133       AA164562   13.50161337   11.85115038   11.90157085   12.70121113   13.97404342   19.51320109   20.14729058   12.69772915       AA486357   19.81244564   19.08483294   9.978745784   13.47424147   16.26643795   17.39386742   17.49834817   12.94888047       AA180742   21.52699749   16.35007638   6.426135031   15.13185304   13.19450881   15.59400571   17.9357522   13.28038761       AA454743   14.91925254   13.9599813   8.65144627   12.90834328   11.96847998   14.68622205   15.68194498   11.59505554       AA437226   14.85387781   17.10133551   12.22946701   13.14384264   15.14400311   17.52701687   17.25793589   10.50190917       AA458849   2.017166995   2.34556391   1.907000939   2.12162749   2.328337653   2.492978456   2.054641085   2.159670081       AA604891   6.821939878   6.718604337   7.304547885   2.689809619   4.718893627   4.373418086   5.049991825   7.871176277       AA609655   33.01277519   29.42862393   30.32802604   37.60777502   42.07314192   25.19427243   25.92346393   17.03793176       AA599158   7.858583214   7.185543001   6.300783712   9.094322938   6.597531463   10.27562393   10.16458119   6.424661999       AA052932   0.253881413   0.171034437   0.788537559   1.088792526   0.303760633   0.25364904   0.134116019   0.728786605       AA769328   0.343955354   0.254618771   0.596171232   0.419246635   0.43081793   0.595702083   0.354185573   1.725014554       AA129537   8.960638915   7.77671267   8.311570259   11.4292329   11.96830695   14.65748429   13.25871143   8.624849205       AA486209   0.130869696   4.19E−02   0.222978133   0.376314559   0.117276599   0.113920001   8.25E−02   0.77617085       H39016   0.14027571   0.094249721   0.182618167   0.2492443   0.135370399   0.271580421   0.149412484   1.0709987       AA464217   0.226422488   7.45E−02   1.63231874   0.357023921   0.235657222   0.260977316   5.92E−02   1.998287174       T95053   0.506118797   0.705352124   2.701833003   0.643138309   0.324882792   0.304836811   0.263668115   1.188075083       AA454640   0.220375821   0.171878877   0.622604377   0.509056108   7.62E−02   3.06E−02   4.12E−02   0.615361756       AA448400   0.43389112   0.405349064   0.482918962   2.543019921   5.42E−02   7.67E−02   5.68E−02   0.744503106       H13691   38.06839644   45.61749341   23.18204722   19.59908539   32.30677452   35.30376824   30.58992766   0.559437392       AA132086   0.368363187   0.158208065   0.357001431   0.998742637   0.108277685   0.210123178   5.56E−02   1.097353089       AA488073   0.223040973   0.324590132   1.411227979   0.464995516   1.253748511   1.167155752   0.478968407   1.88302538       N40945   0.262201912   0.237321497   0.257785251   0.258650997   0.597559834   0.252450904   0.128562307   1.019389108       R55705   0.137145705   9.39E−02   0.38327564   0.365029393   8.79E−02   2.14E−02   1.16E−02   0.572340722       H50114   0.149230356   0.114329742   0.542033448   0.613679831   5.55E−02   1.78E−02   1.81E−02   0.601295897       AA452841   0.241515566   0.336987054   0.216412064   0.47285925   0.332567526   0.409686162   0.241219124   1.10120583       W73790   0.234487599   0.215971925   0.741717434   0.468556893   0.327293291   8.49E−02   0.104656358   0.735173064       N30302   26.09274291   22.41827113   23.15567368   29.55256655   21.88167764   23.38219958   20.81894087   13.76515858       AA291556   0.384633298   0.205779366   0.659149864   0.446933867   0.146800827   0.145065316   0.356438595   1.601652315       AA598510   3.61E−02   2.40E−02   0.108970915   0.102809842   7.85E−02   5.60E−02   0.037519507   0.436123593       AA453787   0.512016161   0.518525655   0.584688061   0.68944295   0.284454929   6.55E−02   5.59E−02   0.634550941       H05655   0.163032728   7.17E−02   0.27016244   0.166433782   10.00E−02   3.94E−02   9.58E−02   0.498009645       AA419177   36.78866172   36.69047817   43.9286988   43.54178839   51.77202168   45.8338345   51.06623927   33.61266646       AA458807   33.10376447   30.88801173   26.67225549   34.5554514   37.93370032   30.31855172   39.9630798   23.50761944       AA293218   26.4028529   35.80834176   26.15447974   33.64819217   31.7917979   29.84313354   26.41862616   17.3599263       W44860   25.75715266   24.52077453   4.553792207   26.21041688   26.01117112   28.71589625   29.44026693   18.26263026       AA629862   34.76420835   33.86130994   30.29052693   26.75522669   33.11708939   36.29104725   43.93948571   27.19603411       AA447674   0.180546405   0.126712928   0.229464734   0.259752602   0.109955437   8.90E−02   6.90E−02   0.339734269       T52484   0.281812925   9.64E−02   0.826718721   0.565508854   0.485893021   0.432859528   0.061805942   0.992986281       AA496810   9.34E−02   7.12E−02   6.46E−04   0.295018299   8.08E−02   2.97E−02   2.19E−02   0.446821097       AA486233   0.340410827   0.117869448   1.53377804   0.356012024   0.538022414   0.203021316   0.156983718   1.084397595       AA079775   0.439060283   0.619002395   0.9808177   1.409206936   0.296042275   0.11668949   0.105478767   0.789070453       W73889   0.126123783   0.64173358   2.015296373   0.918137122   2.418583185   8.49E−02   0.815254724   2.695275623       R50337   2.97E−02   2.35E−02   0.295641965   0.133290557   2.04E−02   −3.11E−05   1.07E−02   0.398363748       R55046   3.121779976   3.583304812   1.420346967   1.371280523   2.205699254   3.267553012   3.02014176   3.938693805       R46821   2.400280379   2.776566441   1.281366833   1.119135092   2.939835087   4.354026018   3.384328112   4.379616557       R87763   0.132976244   0.106549764   0.245268993   1.030976231   8.11E−02   0.130698981   5.81E−02   1.214742481       H89583   0.18904047   0.101052954   0.236746017   0.351564341   9.66E−02   0.145392235   3.87E−02   0.781599712       R56040   2.785909659   2.969666922   10.5096934   1.626253524   1.779842426   1.69343824   1.550682609   3.471096224       AA922705   0.161006175   0.118245397       0.426100265   0.119115471   3.86E−02   0.02671598   1.293134938       AA487571   0.135236965   0.118211417   0.99308562   0.328940183   8.72E−02   2.81E−02   3.65E−02   0.965119113       AA402440   0.406400795   0.454162321   1.143777395   0.734135081   0.607411285   0.139247414   9.06E−02   1.2521297       H29521   0.292621042   0.214822388   0.371852071   0.98238363   0.127904929   0.150473865   0.25566945   0.418395022       AA490911   21.06562623   18.4520925   18.3142956   15.8556682   20.57635735   22.99182529   24.71655205   12.4435305       AA486082   23.15717253   22.55169547   22.5377778   21.98212686   26.33492887   25.7856523   27.61639941   17.1571581       AA678065   43.1549231   49.75180877   44.95138637   30.65381761   40.86775329   42.55581559   44.82447844   19.44908824       R43509   27.41149216   22.05072953   25.03729443   27.50714533   32.1008748   21.09382826   22.18972333   15.16640501       N57653   31.9568203   30.32164198   22.03248851   27.95239629   27.75233699   28.33450066   32.56698428   19.23600632       AA670955   27.613137   30.15379516   27.7763484   26.77163594   19.30516442   23.91926132   25.65098101   15.57144585       R14692   0.210407505   6.26E−02   1.409022733   0.795196983   0.677281805   0.581851815   0.140894744   17.15858056       AA488979   21.80183287   22.74108924   21.96333121   15.76906128   18.73408294   20.2709434   23.79553223   13.91426041       AA443630   0.100651283   9.85E−02   4.85E−03   0.234647143   3.12E−02   2.15E−02   1.05E−02   9.71E−02       AA027840   11.00979974   13.09339183   11.2816884   9.089927285   14.0930363   14.886840831   16.74903887   9.439049175       AA458830   11.37202854   11.65474155   6.607372436   7.389629431   10.78058971   10.57865908   11.80737106   9.428876044       AA459015   22.13388102   21.94307144   21.95512611   28.03858305   24.90291416   27.82736689   24.89782479   18.16821948       AA074446   29.21687173   22.22581916   16.8371288   24.23743962   26.33743534   26.81890595   25.69413696   18.03496456       AA027042   24.22764975   23.72047212   25.83781841   23.88440587   24.95924388   21.01386997   22.28219305   15.37688987       AA629923   20.68133909   17.84071537   18.57653108   22.21025049   25.34003259   22.25297762   25.08441753   12.30816863       AA460830   18.35934471   16.37670009   16.4942895   20.66510843   21.27569752   22.24115944   20.1395633   14.54358761       AA454218   4.705025791   5.029967147   5.2176009   6.528355913   6.43699759   3.661787064   3.143045096   3.120347736       AA046523   6.972538399   8.239721684   8.685210367   9.087650516   10.97162504   10.44142681   9.712940248   6.973860192       R51346   9.268892528   11.49106845   6.177160828   7.887574405   8.018087028   10.44726109   9.783512947   0.215474051       AA029964   7.968348913   10.02474618   7.998306197   7.953245463   6.008361088   14.22552591   13.76159965   9.150938402       AA489219   3.977503262   4.357159927   3.843713092   6.387649039   5.182503022   4.084539228   4.217440316   3.929360037       AA043133   7.10915371   6.657783457   7.232430397   9.095892814   7.495998702   8.296677078   7.86458314   5.986265513       AA812973   31.52455964   28.41615616   25.69771578   32.72534183   34.85110642   31.04591265   32.76410913   19.46276288       AA453471   9.736208184   11.1890541   2.985624257   6.304114807   6.507046353   13.54093424   11.82320488   11.84074045       AA284693   22.56815563   31.15842013   22.14580038   30.90223481   26.73160123   32.27184876   29.98195912   19.10255765       N90281   6.43341783   5.959020565   4.766657998   6.027996022   4.863654532   5.006421597   5.844930445   3.835280867       AA629542   7.506542166   7.498548839   5.126718959   5.758458157   5.628204957   10.92011362   10.66563175   8.830974583       AA679345   11.61400851   12.1850373   0.318260632   7.086444187   10.6336761   12.99915052   14.28658079   11.11062364       H37774   2.83E−02   5.37E−02   0.455840137   0.23068029   0.027217407   8.89E−03   7.83E−03   0.143799652       T97181   1.876643541   0.587861517   0.977072756   0.374576034   1.401186113   0.980966491   0.307497525   0.977649837       AA454879   0.128788844   5.49E−02   0.642103913   0.314369938   0.171773759   6.95E−02   6.83E−02   0.364117574       AA147640   11.10684686   11.11067304   4.334390147   6.745732625   7.498713228   9.714345375   10.05962462   9.682475093       AA767429   13.9665151   17.25513268   9.348363447   14.61003519   13.1757222   18.11092619   17.6813815   12.40577438       AA490991   19.30297964   18.58197931   18.34934113   17.62227921   19.95904418   22.8372929   20.13821173   14.51945376       AA422058   31.53896803   26.94893157   19.67019378   29.90401606   24.82963178   26.86419627   30.12270888   20.6779599       N66208   15.78244935   17.89542247   17.81439029   14.63969059   15.54037985   19.14257856   19.46042164   12.13187951       AA630776   16.29858697   16.75035086   16.12331467   15.92787633   17.52684563   20.53155822   17.53930107   12.98947212       AA827287   39.31448991   30.4334384   11.88952147   17.38709762   17.86731786   24.85586178   34.18834198   20.6039679       AA488084   14.20448178   13.42950914   12.7036246   18.9618   14.49387186   17.34183829   18.41774811   10.91028856       R89715   19.00984517   16.78226171   15.93191526   23.59033847   19.8478327   20.09882291   20.88101303   13.98761153       AA490501   17.29764034   18.48394335   14.52922019   15.77576344   14.10802818   23.00662243   25.37702488   14.90184018       N32199   27.83247395   27.16433957   27.83599929   17.63576421   20.01897529   29.53277745   35.16676364   20.63729373       AA434404   12.98452113   16.11788081   13.26212785   17.45606094   16.25005553   12.59970441   11.14968041   6.811542045       N93688   28.28483481   25.54412177   19.80975005   17.76258329   17.42508421   22.88245448   31.60912628   17.69865454       AA292676   9.876235182   8.317319028   3.210728167   4.436837751   4.953957507   9.622367291   7.40323057   7.63997429       AA464417   11.05434122   11.16528302   7.715140755   4.628342903   7.257940789   14.12507957   12.84862626   10.51986876       AA442092   5.48651812   6.31065156   4.641902691   4.599175502   3.885904241   4.692019402   4.440172458   3.114766105       AA442092   5.48651812   6.31065156   4.641902691   4.599175502   3.885904241   4.692019402   4.440172458   3.114766105       AA026644   0.62252822   0.701896284   0.261093119   4.191592598   6.49E−02   0.029204697   3.36E−02   0.437058833       AA481464   18.63468043   20.94766373   14.27769307   15.75348631   11.46364273   12.65501458   9.009332352   10.36580755       T68859   19.81384726   21.31173502   19.75635729   20.39980475   20.05062771   19.46428757   19.33000277   13.65552685       AA699560   20.82023648   16.7911717   18.43819663   25.52023654   19.85201903   17.12500341   12.21472729   10.2413573       AA705069   20.88685524   23.16148711   15.0840977   20.67586612   14.18817428   19.01580429   13.44540219   13.6412039       AA457739   33.37327991   35.6883041   34.4823112   20.55802853   31.64214174   37.33300573   37.02468042   24.08688689       H99843   16.79713092   16.69352615   7.127333563   18.73583293   9.995156003   14.60344228   16.5049794   12.29468204       AA399410   29.72998467   33.11698129   23.43060422   25.72188937   29.04178273   34.63630675   33.29299911   21.29525921       AA443039   17.93908822   18.28745065   29.34817189   13.16317634   17.21193969   18.81890352   17.62059907   13.67676164       AA164440   9.30576802   8.984327957   9.868215304   8.433442763   8.752113579   8.431813452   5.330326944   5.313034392       AA446453   12.86153172   14.28408559   6.56088494   7.913354462   9.798358874   11.52903302   10.6491586   9.31631564       AA280692   13.47620777   13.91271582   10.59679067   8.217327335   12.09365638   12.02460496   17.61991673   10.08089846       AA031514   12.90000518   15.02943076   7.652758135   8.941687918   10.4641265   13.93556692   15.68826294   11.63565104       R33154   15.95001951   18.20484579   10.1215106   7.276840559   13.99489447   13.57306878   14.10166919   10.83686664       AA487452   8.187976148   8.458602154   9.876629011   5.590565736   5.757265048   9.281445194   7.000673633   5.618451692       AA400329   18.57548571   17.4980898   9.303751927   13.38561433   13.86149745   18.50617543   19.11656532   13.89116259       AA454668   25.89304171   31.51348528   22.93446371   22.57583786   26.88624881   28.81055744   19.75055767   14.91854781       AA486393   0.182124982   0.615575177   4.602814218   1.268031115   0.231530443   0.120506359   0.294243975   1.047822638       R62541   6.08E−02   8.40E−02   0.282420426   1.89326344   0.22723694   0.55787894   0.265300367   0.465287427       AA171613   0.27466036   1.123906401   5.619710529   1.64076918   0.206048231   0.318509847   0.213712273   0.784934532       AA235706   57.38142594   60.53251272   55.52482329   61.56762824   83.23998616   63.03392579   63.61974345   26.90748839       AA668527   0.3985816   0.597438821   4.966520787   1.47871208   0.198743181   0.088338506   5.14E−02   1.584106321       T64144   9.38E−02   0.192484503   1.850866515   0.17162783   6.49E−02   4.08E−02   0.166840739   0.696340115       R14080   9.55E−02   0.107642909   1.600491683   0.379619373   0.14791746   9.50E−02   0.055410162   0.820779184       AA609599   0.187186514   0.10012971   0.27386765   1.420850931   0.186769847   0.275819631   0.221567336   0.689208446       AA489201   0.14443693   0.214598987   2.006534993   0.32146219   9.07E−02   4.93E−02   0.063098942   0.80944825       R08876   0.568537573   0.970149717   1.576770394   0.384565828   0.43616572   0.422480014   0.296461364   1.181968079       H46425   0.12265004   7.01E−02   6.84E−02   1.742654009   0.150967538   0.162468773   0.155852088   0.251147304       R56149   0.139454424   0.213035184   0.815295152   0.482793426   0.717693865   0.297475848   3.81E−02   2.113668202       AA454619   3.288336383   3.22099397   1.915196108   1.919736037   2.762560824   3.92242617   3.716888609   5.217509513       H15445   0.120219632   0.111757383   −3.02E−02   0.467435552   4.96E−02   1.52E−02   9.01E−02   0.41450502       AA705225   0.18852753   0.180935638   0.94755028   0.256955531   0.098583152   4.21E−02   2.79E−02   0.674486801       AA191488   0.8106325   1.339071481   9.778098245   9.71763194   0.472492022   0.577988082   0.368988511   1.716820958       N64862   0.177514818   0.134938261   0.724810552   0.540552309   0.142613803   8.88E−02   8.34E−02   1.380235208       R45413   0.121785119   0.177364842   0.66590467   2.22765821   8.94E−02   0.189579838   0.126874076   0.639711527       R77293   17.32761828   18.58361324   4.930581246   11.25376919   13.83440333   12.64389145   12.77350206   10.14808689       AA436187   15.01390271   13.59205508   9.447023349   23.31188756   9.068747717   12.4718263   13.46398542   8.219025894       AA676470   30.08495758   24.12483149   26.19627013   36.68374593   38.36383944   30.07336868   29.97197759   18.93190315       AA443634   8.40E−02   0.248574668   1.87808441   0.506000681   0.115582035   2.84E−02   1.86E−02   0.530955426       AA664180   36.06326367   31.7886287   31.69253658   48.48811822   47.96493814   35.23111673   43.94952253   18.50826673       W58658   0.173902417   0.15340018   0.620011724   1.529093442   0.023894127   1.68E−02   1.45E−02   0.702231009       H54023   0.247467714   0.188125663   0.227984638   5.912011779   0.223991418   0.399206145   0.253395926   1.386292307       H73724   0.088814629   8.03E−02   0.231928251   1.683504664   0.117575022   0.155606877   0.129032488   0.444812754       T70031   0.277245911   0.148769574   7.22E−02   6.834546017   0.22727827   7.26E−02   4.66E−02   4.39E−02       AA481758   69.05370315   37.39817347   42.70517496   58.7187498   64.37630302   40.66376745   37.7884949   23.04551033       AA521431   61.83605618   43.27436449   55.26120705   77.90743727   76.13351238   40.95637623   42.00937518   25.13021963       AA446103   0.104636512   7.03E−02   7.95E−02   1.215881577   9.41E−02   0.149593049   0.125073625   0.646399796       N92646   28.06784683   30.96204311   27.96134068   36.82918822   30.95508803   36.04823224   35.37693112   18.89015759       AA453789   1.808404003   2.62249449   2.812909827   1.746220767   2.577090448   1.40223369   1.246611512   3.36017901       AA425299   13.08032469   13.71772041   14.28834182   16.54671153   17.41154591   18.68745962   13.52405931   12.63625711       AA868929   0.618942483   0.614412924   0.22361388   0.834682507   0.635222734   0.370649638   0.37352773   1.258217182       R60019   0.119841725   0.116811729   0.259384551   3.00499051   0.182169515   0.151089307   0.155852498   0.941525731       AA857343   1.901017437   2.489976812   0.714479672   1.232686475   0.932256949   1.974939699   1.554741816   1.884138006       AA481438   1.201789068   1.353488237   0.871369975   1.070764965   0.757366565   0.88003401   0.80663823   2.827484886       AA399674   1.105347969   1.501678874   0.866552209   0.74069806   0.55317612   0.938530357   0.733003255   1.646361253       T98887   0.124035858   0.312987764   2.343639547   0.401768082   0.207652428   0.506081312   0.158684645   0.746649314       AA678404   2.36841611   3.938382636   1.58773702   2.194204209   2.061478452   3.607585825   3.37665162   3.542744541       H15747   0.287743257   0.229134462   0.372656688   1.777298932   4.66E−02   5.65E−02   0.041067986   1.289613816       H16958   1.894931477   3.000533791       8.633043094   3.547286301   1.619498817   5.733681715   1.283158823       AA936783   0.389167344   1.499581511   10.64077637   2.384094632   0.13911189   0.66026872   0.277728232   1.169970181       AA884709   2.198015928   2.066757874   1.718800124   1.980966588   2.355838772   0.830886735   0.335287796   4.749490887       H24688   0.201982284   0.219962769   0.751879444   0.512830988   0.153838277   0.734814109   7.99E−02   1.552613702       AA884403   2.103460786   2.672782071   1.300637897   1.099433595   1.733827895   1.274946177   1.242053454   2.973138744       AA404619   0.15138684   0.357518214   3.52385316   0.69841884   0.179573378   0.18311414   0.182224032   0.75943341       AA598611   0.330074441   0.226908429   1.350802268   1.655404779   0.200113403   0.186066173   0.124232766   1.678785386       H72875   0.179741117   0.13827264   0.473352965   0.490771985   7.61E−02   4.42E−02   4.34E−02   1.006169064       H63361   0.163571849   0.12317033   0.475111201   1.012711843   0.119954697   0.119944558   0.113665578   0.624304289       R39221   0.186857808   0.316450834   0.432784395   2.516001904   0.671600175   0.711850096   9.23E−02   1.36227562       R02348   6.20E−02   0.206438723   2.150640832   0.255419744   3.89E−02   2.30E−02   3.21E−02   1.140843608       R51835   3.03E−02   0.363378683   0.731237551   0.726185228   1.322490099   1.631249806   0.224518258   6.856746548       R33031   9.98E−02   0.162983983   1.827344826   0.62547274   0.062236534   3.12E−02   7.42E−02   0.817377295       AA412053   0.204788914   0.413840453   1.348709648   0.357745167   1.241913181   0.757293992   0.22139368   1.438145557       AA001897   0.348034638   0.171387974   0.207561282   1.765380433   0.543185967   0.658838523   6.58E−02   0.748430368       W81191   1.790574222   1.899247237       1.807214492   0.346308978   0.222762323   0.107712128   1.34541522       AA430552   2.91607572   3.076041003   2.829597507   4.426370714   4.729925458   4.71187164   4.531847561   3.70805471       AA594130   0.703542309   1.005837145   0.525583509   1.216578849   0.691717084   0.376548847   0.27857931   0.635247713       N92864   0.079866033   0.112472409   0.251854929   0.452282211   0.101581389   0.227029764   0.237170156   0.466949248       AA457123   0.216922531   0.155819343   0.364362183   0.723388363   0.133198078   7.45E−02   0.10617339   0.52975191       R43320   9.13E−02   7.59E−02   0.458730796   2.051917813   0.104074549   0.137491428   0.108125471   1.014817239       AA670430   9.678888892   6.883278692   5.717565814   7.613099058   8.65181804   7.995091885   4.8996271   5.249095284       H85068   0.34278548   0.256922183   0.586815168   4.298907537   0.476155756   0.479705389   6.74E−02   1.020767749       AA458785   0.785442166   1.506491022   9.808733277   1.828902106   0.920171532   0.658849251   0.698071385   1.994825669       AA485871   0.597816889   1.768341753   11.84398613   2.137826817   0.262843105   0.254992284   0.126660693   1.303743017       T39411   0.396231188   0.296618072   0.324548562   1.969945161   0.169337341   0.268104021   0.247950339   1.580361635       R00855   1.087364016   1.024237951   0.765054603   0.975300349   0.539506121   0.412648188   0.154076211   0.733947191       H98666   2.109706251   2.780481372   1.275784185   1.529882866   1.784602477   2.774145853   1.966370637   3.844830747       H72028   0.181183944   0.425147123   0.857472955   1.001641974   0.163941215   0.967420557   0.351793709   0.907756492       AA679177   0.348135481   0.500333332   1.326832638   4.439877031   0.32836439   0.421388335   0.341562345   1.848279914       N21578   0.168101047   0.117805103   0.409669051   1.325818704   8.67E−02   2.22E−02   2.56E−02   0.494287318       AA007419   0.175440269   0.12813069   0.39815608   0.955234641   0.161389912   0.196276298   0.170532566   1.071322887       T49657   0.308720496   0.570684257   4.039761403   1.234676181   0.125959577   3.27E−02   1.97E−02   1.338939336       N38959   7.55E−02   1.055602229   7.313895345   1.268404297   0.307559568   0.724991758   0.070393719   1.035781395       R51912   0.30750311   0.157082387   0.297430002   3.304783027   0.316950074   0.28696647   0.164570106   1.141683339       H90415   0.332923734   1.116687454   9.324562689   1.997045708   0.350224199   0.100034162   8.78E−02   1.169187709       H41489   0.135065338   0.112846912   0.31963146   0.540685076   0.105448274   2.38E−02   2.54E−02   0.533589747       H16456   0.118493184   8.15E−02   0.670523766   0.212363873   9.91E−02   0.048036092   3.66E−02   1.044685298       W45415   0.187478377   5.20E−02   0.722058962   0.692564761   0.293080465   0.189933858   7.54E−02   0.655830442       AA447751   0.78396734   0.786015622   0.528464235   1.211081391   0.239987152   0.17186979   0.272698223   1.030511383       AA487486   0.534940441   0.350108402   1.666390776   0.795295661   0.359287561   0.193793075   0.198174164   1.934418861       R56604   0.3460236   0.406195433   1.083709289   0.653908131   0.50487088   0.489048814   0.385606236   1.682176098       T65772   0.25838637   0.106483973   0.235991068   0.28337251   0.46880307   0.109303057   0.116698413   1.388384089       H15085   0.157580914   0.114768422   0.508468022   0.673401507   0.17757899   6.65E−02   6.08E−02   0.136004744       R61295   0.212944574   0.159174688   1.555313951   0.607244235   0.169955202   0.225692165   0.172935079   0.384976032       T61256   7.25E−02   0.151774275   0.761713647   0.145248528   8.64E−02   7.32E−02   6.14E−02   1.055956615       AA405731   0.195114794   0.136469531   0.339111129   3.35219508   0.125085325   0.189941763   0.145965169   0.612889025       T71879   0.128933321   0.137341443   0.390507256   0.407085926   0.114225823   4.49E−02   2.40E−02   0.521752069       R59927   9.10E−02   0.107392035   8.50E−02   0.442229621   9.27E−02   6.81E−02   9.43E−02   0.347354052       AA496780   0.110593994   0.022497663   0.534503826   1.752767599   0.281792243   0.415100922   8.75E−02   1.131163315       AA176888   0.178362771   0.144369523   0.781888663   1.331812939   0.187224487   9.28E−02   6.82E−02   0.650312155       AA436163   0.23227633   0.236489348   0.254658187   2.416941823   0.161958036   0.3988222   0.305725744   1.256999654       AA428778   0.105802992   9.28E−02   0.486961912   2.193301764   0.163889027   0.132381001   7.25E−02   0.611181122       AA463225   0.276223908   0.152997555   1.039475243   2.155030865   0.418387856   0.329192564   0.19424438   1.916291602       AA485426   0.289852714   0.373831514   0.418062891   1.661826021   0.22603134   0.194563224   0.121355588   1.266933317       W47485   0.290711332   0.28058818   0.290834133   1.405085787   0.21336519   0.172813844   2.02E−02   0.962779151       H84982   0.131639592   0.139838613   0.317575218   2.016302257   9.62E−02   0.376525133   9.28E−02   0.503147875       AA504615   0.260955437   0.211851667   0.911938546   0.668568083   0.134885637   0.121344263   0.121161862   1.972320295       H94487   0.176887945   0.365682094   0.630918749   2.620578978   0.183460578   0.419558641   0.179194474   1.679828681       AA448959   2.916692897   2.932033692   1.637325243   13.34753362   0.628009453   0.180111066   0.132777658   2.07701127       AA070358   4.54E−02   4.12E−02   0.367054172   0.116479427   4.89E−02   2.03E−02   2.61E−02   0.244403144       AA453401   0.167660345   0.214972768   0.222252985   0.203904483   0.242997298   0.251569524   0.177121661   0.804209416       N66737   4.20E−02   7.41E−02   0.257268707   26.36051857   1.048190646   0.091475566   0.104092386   0.79476014       AA666180   3.196631019   7.187377922   29.76323485   10.64207727   2.761542964   3.990641982   4.044434382   4.868184378       AA857131   0.223573307   9.63E−02   0.758187241   23.03526077   0.070686238   3.60E−02   4.56E−02   0.878719343       AA479102   6.852949035   8.120715713   3.236793772   11.51815786   4.73539654   7.003983588   6.605548634   5.888262807       AA456077   1.027879631   1.325946518   1.245447159   2.318900816   1.481005766   0.592776193   0.435487259   1.471236654       R87497   0.319714784   0.441278788   1.241443943   2.794768846   5.52E−02   2.59E−02   7.17E−02   1.35489803       AA718910   0.771846813   0.859478249   0.801090192   1.362044268   0.616842421   0.429437896   0.408760631   1.063445986       AA406269   0.270836576   0.224358482   1.06080255   1.245738874   0.124219479   6.82E−02   9.79E−02   0.972478072       N74623   0.147826314   0.155576422   0.39183835   7.661750863   0.116891312   4.48E−02   3.09E−02   0.687478091       H99364   0.547513791   0.368774275   1.09104024   1.578071783   0.315822232   0.108583159   0.10216456   1.465520886       AA447684   0.497110821   0.537175623   0.525424572   1.458911244   4.98E−02   2.74E−02   5.04E−02   0.735202872       AA282301   0.27173204   6.68E−02   0.520517404   3.191486412   0.722232552   1.277477958   0.144496875   2.236318764       H99588   0.227706892   0.166835375   0.379322092   7.180929201   0.205434573   0.247795568   0.187784708   1.219437291       N53512   0.693352824   0.85093842   0.861714576   5.362013301   0.478594275   0.673037164   0.544521467   1.733748427       AA683321   0.246596976   0.176033115   0.293208249   5.563771987   0.123251357   0.226202587   0.181288584   1.448843062       AA608557   0.385872016   0.383796565   0.952099841   3.879470723   0.316768638   0.312030768   0.238687939   1.726847773       AA757764   0.297379076   0.165726325   0.88261567   0.440905543   0.372520434   0.382055076   0.145493758   0.988760934       AA406064   0.963197456   1.184985842   0.779087405   0.891603525   0.966885185   0.579894823   0.330253413   1.152159636       N54598   29.7307313   23.60460158   15.15010606   61.1100551   21.20150657   23.73876744   23.56652312   20.42284808       AA481988   0.83030089   1.67920494   1.521390918   1.602447026   1.594545777   1.393612985   0.586783337   1.454014827       N62394   0.292745156   0.145445316   0.919547638   0.486071328   0.618336636   0.192065885   0.209279951   1.81964171       N20148   0.36264957   0.293240313   1.191190173   0.634599551   0.725999262   0.465883026   1.173321753   2.675970931       AA496678   0.319441345   0.257667574   0.478071395   0.622386812   0.132931879   1.95E−02   2.60E−02   0.840188206       AA400973   1.534002053   1.714822504   1.436906175   1.048156955   1.254760066   0.941922627   0.722913601   1.95298952       AA497027   0.438480922   1.508084468   0.372150077   3.020669538   0.359906823   0.227232464   0.14420936   1.043280575       N64508   2.009112662   3.452207016   0.792805621   1.301784349   2.539009334   3.500903196   3.083422958   4.592914592       AA033564   0.069421532   0.161663182   0.323575075   0.250293874   0.17400072   0.226600648   9.11E−02   0.871274685       AA446108   0.769668027   0.390512087   4.097425592   1.207299335   0.354854654   0.436418383   0.134273293   1.258329856       AA159577   0.446474167   0.379318202       1.090721231   0.206696214   4.62E−02   3.77E−02   0.916706477       R36958   5.23E−02   2.39E−02   0.262096407   0.139051554   0.101530951   5.47E−02   4.12E−02   0.458047702       AA829508   0.630754179   1.081371373   11.12264238   0.264354582   0.128126994   0.765515506   1.149526797   3.042979924       AA482067   0.205039081   0.253788068   0.282480027   0.493737706   0.158658574   7.90E−02   5.28E−02   0.55095889       AA669314   0.683742215   0.808392548   1.16372394   1.602848914   0.442885133   0.570227867   0.280240919   0.712164672       AA775241   1.418874982   1.846198647   5.577800931   1.344689512   0.880185699   1.252484239   1.424573602   3.384055572       R73584   1.02446963   0.935468411   0.803404707   0.368215322   0.286257374   0.109893655   7.53E−02   1.275588944       H28984   16.59350558   16.18200978   8.172410684   13.1191606   14.4503845   11.12950391   7.932771347   9.633157025       R44202   8.69E−02   4.382−02   0.253533697   0.166629344   0.117904267   0.101127842   0.103847056   1.059451655       W70051   21.76297737   20.04405194   14.91138327   16.87667976   19.87488409   28.04573416   30.08594829   16.42056168       AA401972   14.92249068   16.42885435   5.611565027   8.854697045   13.81855051   16.92468552   18.75158154   15.74888433       AA236164   20.21677625   18.79310438   12.57587929   11.95096823   16.36247541   20.16185377   20.77848532   15.16538035       R22412   15.72505574   17.55060555   15.82656015   13.78588969   13.30933358   14.5213203   15.01308178   13.15195793       AA424804   14.70872577   15.97533753   10.78900463   13.01835661   12.64309311   15.36509292   16.14117886   11.65209772       AA669443   16.40914268   14.22534282   9.970977767   18.06831801   15.3193005   15.5104445   18.01732542   11.20069358       N69689   11.40467488   11.65322402   6.372712462   5.538040372   6.533556933   11.1584167   11.60779004   8.597842756       H24316   16.83164161   15.705615   15.68021927   15.56699579   19.40991358   20.01447688   19.29795097   0.497864329       AA074224   0.333624301   0.375913694   0.378626744   3.784608939   0.327739524   0.489769841   0.322978295   1.313192294       R36571   15.28422925   17.32979473   15.34324385   15.25547675   18.55691796   14.2447373   10.72233164   1.060647719       AA058465   18.42366544   15.89605854   8.341211937   14.29501218   17.71874821   13.05741368   16.65715974   11.02252852       AA633811   22.16035468   22.782521   30.6526342   14.91020806   19.44240872   23.66144082   26.37266059   17.24459371       AA457155   21.68263476   12.69269947   15.02783414   18.54841663   19.13660886   15.98507842   11.33190117   10.09290381       AA459104   15.2195866   16.20194954   24.24665432   13.86213005   18.26771629   15.71979923   13.29493507   12.57587877       R40212   14.5790754   17.12092824   17.93072196   13.93477495   17.42255813   17.93870181   18.31265802   2.2735197       AA086476   17.74782242   18.69369476   14.58097392   18.97637683   20.8754483   20.68533459   19.26034455   12.52733321       AA683310   18.34330926   18.98378867   6.057610695   11.98270843   15.69453266   15.19045985   18.66892542   11.4721218       AA455640   18.47809437   17.61623038   7.452925146   8.994012338   8.980063651   12.54788533   16.22279878   12.11311137       AA496878   5.246470108   6.492383676   7.23111917   5.567148567   7.297448165   6.519473799   6.047516043   4.881896262       AA085749   10.15319844   10.58867058   3.720477618   4.092990024   7.461499363   6.388572117   7.203726545   6.728320601       AA425755   13.24455202   12.88357607   5.034714528   6.268355719   9.082441534   11.59487571   11.62952447   9.529595044       N52350   10.61031686   10.79705267   12.63852019   8.6754759986   8.976553332   3.221438906   2.335184873   1.494699625       AA630104   12.7227255   13.72066077   6.71563807   7.938528099   6.913277923   14.18776318   13.90026724   11.35565277       AA454854   10.92967966   11.23143802   4.418447748   5.174772829   7.976384214   9.703784616   10.93778907   9.331503041       W73406   7.903009948   8.279041624   2.830978933   2.857181738   4.639918805   4.149802613   3.606526999   4.719619102       R12802   0.238444224   0.16825892   1.58807476   0.822064291   7.205178124   0.257279583   0.444604195   1.264969686       AA465355   7.336819392   5.300937536   2.387694789   1.77122396   6.072708998   2.990994851   3.612925236   6.445676951       AA829383   9.713113247   10.66886848   10.54302042   11.63031161   8.843010597   9.775032469   9.586544135   5.681120215       AA629189   11.36797877   14.967791   4.768128161   7.170347339   9.57906376   11.61769675   10.65243226   8.340390966       AA430512   9.534573517   8.247953524   8.316348291   9.290581043   11.64863395   8.113650285   5.996962103   6.214691332       AA456439   10.70832344   10.50311685   10.50165717   9.291870768   11.97285965   10.03817061   8.954783916   6.730899031       H27864   9.780274543   10.17961553   7.795175273   8.32114728   10.79728458   9.655471293   7.995744552   7.171240518       AA644657   14.97773748   14.72866784   15.19497586   17.4722782   14.48792372   13.4943961   14.66848169   8.981574288       R40460   14.02006989   13.67189221   6.32559769   8.741499108   13.22962391   14.28706841   14.74331878   0.86098332       W96058   8.316574942   9.202396521   4.185436741   5.802080978   9.090198422   6.944350085   7.898182283   6.501582684       T72202   4.910901674   5.633601899   5.685609621   4.651309743   4.997212679   7.505442874   5.542126135   4.689359807       AA598794   0.573341181   8.49E−02   1.038303088   0.244900677   0.310786175   7.77E−02   0.048832125   0.163149057       AA599178   0.570691633   0.339390237   8.557633935   0.480744514   0.435231923   0.218741059   0.216046798   0.435225613       R88247   0.532639965   0.545118994   0.620081258   3.141140933   0.150055455   2.45E−02   1.85E−02   0.7022889       T98812   0.343184973   0.247595029   1.531771465   0.291419246   0.128461144   0.340974608   0.198086927   0.425481979       AA454858   0.223635364   0.268810127   0.8147468807   0.353760373   0.103986984   3.73E−02   3.16E−02   0.245535885       N67048   10.14341863   7.592113899   4.344522356   3.851620223   4.536042504   6.665109387   5.008133377   7.16695928       AA776875   8.06934578   7.7581352   3.091038965   3.741685834   5.546996168   8.791269951   7.982378371   8.365452414       H51117   3.966389223   5.346765011   1.395463454   2.49073858   3.687011927   3.718900078   3.084623398   3.524676899       N36174   14.483682   13.54683346   11.11466575   11.63388016   14.05262104   12.75178351   11.89006855   9.330359142       AA777187   10.42303629   9.748871101   3.642022531   3.229088085   5.366152041   5.862550486   5.823237791   8.241060625       R09581   22.00681959   21.11752477   18.04191799   22.91566491   22.79559771   14.44791388   11.45011661   12.16467252       R16849   8.612396808   7.596990399   4.924342984   3.325162711   4.110023154   7.765585109   7.820823897   5.815135246       AA884167   4.188050396   4.749402103   1.61148811   2.749913448   4.2780267   4.100967576   3.815787701   4.055039882       AA136983   9.49585425   10.15056984   3.440351838   4.615880778   6.054186478   6.83657547   7.050636979   8.269159968       AA488622   9.890487467   9.836219264   3.473509988   3.789767104   6.282000648   8.049767976   6.973213882   8.212583544       AA699427   7.117192529   7.111662492   2.192838503   3.551786441   5.580591133   4.198944242   5.074757452   4.209708436       AA490459   2.575656166   3.736948711   0.933603593   1.465812184   1.758713261   1.104333244   0.867383854   1.531572668       AA626787   2.881272998   4.299097477   2.721322605   1.916838494   4.469467633   5.38359605   5.46846531   6.322351973       N62179   11.37102878   9.568317921   9.455248318   13.56398276   10.81112759   10.64826613   8.56567794   6.723636949       N27190   29.54707234   26.25035232   31.35363406   32.67661467   37.57564217   31.43271623   31.53461788   20.77058355       AA441895   24.27150375   22.9626093   26.60631666   30.86801888   40.06670353   29.78805939   34.54349576   17.24254068       AA463924   33.402247   33.89347601   35.571325   30.50800393   34.84762552   32.77696512   34.17207689   20.5248861       N78843   38.02091306   31.39298491   28.23461359   34.41711929   39.51187698   30.11933147   26.82888212   19.43620727       AA629719   31.48764197   37.41727747   31.54660297   39.80567516   40.1563207   39.38844138   30.25084933   26.0742679       AA404755   21.41973277   21.24106888   18.96936278   32.4046278   34.53593287   19.71196965   18.51583687   14.25627129       AA459351   1.833795538   2.618617925   1.373480533   1.256440944   1.67228768   2.914913803   2.591977948   3.899562773       AA488346   39.32983741   30.32593937   33.03970317   45.78348359   38.26709796   28.22982528   30.16584203   18.03374987       AA427899   26.67604716   25.78523385   30.14609328   28.85287545   35.98104596   35.22864254   36.82338782   23.94024054       AA453813   7.204336211   7.303817317   4.900254154   4.379972352   6.206941047   8.824169559   9.98471585   7.351059946       AA397824   10.76945162   7.982251483   7.580720116   7.432531008   9.754354143   9.514915642   7.935720221   5.920549076       AA633901   12.18200405   10.85308114   13.61633796   14.24924715   11.46202942   11.95459476   10.74415658   7.891587608       AA181334   9.69993387   10.26903792   7.998753206   7.736489528   8.536351201   10.14387035   10.00804431   6.84688368       AA292410   18.41498084   21.39567723   7.43645534   9.83113847   10.29990367   18.49023492   19.65621623   13.96412248       AA253434   13.4092855   14.33475919   8.039460846   8.613564071   7.929641139   18.67319453   20.17449968   12.27802285       AA455058   20.82871509   29.27828702   14.29348739   29.3258109   37.85290764   27.56973385   34.99886458   17.91174019       R55188   53.79096202   48.27594603   54.21416245   67.09649695   76.87450563   52.63193582   55.51472182   23.62696499       AA465723   23.38181778   23.20566188   14.35096285   16.08639335   12.51504107   18.1389079   19.37029588   13.58503588       N49856   29.96970494   27.03519888   26.29856571   32.89736617   28.41338729   33.76040637   41.23242017   23.20296225       AA455272   23.70820419   24.87271063   17.74038543   15.81791403   21.42687241   25.95981278   30.31298784   20.80534628       AA459292   25.08753086   29.85942844   24.33982097   34.38597901   33.16899531   29.38762558   29.07865921   16.30430876       AA878561   29.70997223   38.1181983   42.41594013   29.19560183   37.16368231   23.80169892   29.15914586   1.513482669       AA772068   43.30930177   39.39007192   42.20898479   60.0670886   41.1840771   50.78509048   59.03512613   28.26604168       N78821   31.19009514   31.82358739   34.25368088   37.21129767   43.16878807   28.5580241   29.24827598   16.3537237       AA291490   45.77501651   46.63476256   53.21442136   56.17239995   68.90492839   54.65064586   57.58008452   35.76767897       N46828   34.89731064   29.39427889   34.05249693   29.87756141   39.01685965   36.99198256   36.92808227   22.17775304       AA150487   45.34684386   44.65368856   65.30284844   57.55943844   67.07416778   40.42215172   41.79371663   27.72434403       AA282537   52.22535751   41.56388709   35.17403314   55.2750426   63.04486445   34.94861701   35.66047764   23.4861499       AA707922   55.46799902   54.73165612   52.18006189   74.92853541   77.27931598   44.41763762   48.05962465   28.42806546       AA443638   47.23582949   41.25447374   31.90537257   20.13909587   55.94493635   32.20561704   43.85324507   23.67050394       W73892   20.27131765   22.07700187   17.82340994   12.42664249   13.03977036   19.26896493   19.76283359   12.52223224       N70734   16.00676831   16.21613077   13.02268984   13.77803964   9.864935146   16.31280635   19.12043735   10.93049868       H57136   29.82885337   23.14775485   17.64717904   22.5131702   27.82013988   19.25424118   19.119368361   16.68407949       AA700414   29.78740203   29.71153223   30.84738122   35.51331266   28.5320194   35.02888476   37.830234117   23.51879387       W65461   1.582316414   2.170422952   1.103228692   1.038420135   1.171524513   1.304498683   1.310369445   2.223501134       AAA36584   9.362091141   8.134132036   6.780573609   9.12471574   10.01008974   10.0915921   7.199616982   5.462605703       AA029042   30.76692002   23.54804667   16.01970787   20.5027043   24.29216348   19.81870818   18.97220606   14.02066164       AA427725   24.1831519   26.60251326   23.95557184   27.87062775   19.53245951   29.94953555   31.50513283   19.2987866       N51280   27.55225851   31.81602093   10.9862251   15.87844486   22.5718942   31.13378929   33.56546319   0.278258082       AA281347   22.81574164   25.60885154   14.8674075   16.0871738   26.21817206   31.27308752   33.3028934   22.75939773       AA402960   24.41874925   26.11670313   18.18713838   19.51273682   24.58402722   27.14137043   33.29284839   22.8496167       N98485   23.15377588   21.24725903   20.03077128   16.99045362   21.34886081   15.26211932   16.22677894   11.5187422       AA490209   3.035698537   2.915638696   1.659834641   1.10758798   2.258178208   2.051133108   1.835282947   3.040695852       W61361   34.10379475   31.17761956   34.01922951   29.43169864   23.02878278   44.28043077   49.3716344   30.55831089       N51018   0.895969739   0.822603424   8.791300555   6.566440211   0.387721776   0.485854727   0.500500102   3.973103926       AA455281   3.982933258   5.842616596   3.409051258   2.155743141   3.214957653   5.761751821   6.1678428   6.595859335       W69471   2.176145649   2.262971009   1.889011302   4.298069671   0.125110544   3.91E−02   4.41E−02   0.885776492       AA486321   1.034914616   1.422616774   15.19920754   7.415723464   1.525470041   1.404546396   1.733369861   4.163095485       AA458982   5.030129846   6.671175844   1.456266641   1.68375405   2.379536052   4.179216222   2.976132273   4.062391389       AA442095   4.829174413   4.615478182   3.252727167   3.668199484   2.808813532   4.180626657   3.959090691   3.356087801       N99003   2.039062485   2.563557284   1.92061982   1.456785844   2.0821831   1.985365512   1.595312179   1.552308165       AA609284   2.220425329   2.885924256   1.15451555   1.264654536   2.17588501   1.856888897   1.575172599   3.007772428       AA195036   31.39371467   32.51612495   19.8134058   22.2287943   29.07492796   24.93265482   25.96878567   17.40551832       AA478268   11.96015041   10.99922076   11.69181426   14.11437595   16.20593802   9.111161437   8.678545684   6.90313221       AA608583   30.64392285   26.66604793   25.63350599   30.51834358   30.33697486   24.55752432   28.90088593   20.25532836       AA480435   21.82366631   20.06886964   18.88262313   22.61456267   25.53193168   21.93073616   21.58624772   13.12039825       AA505046   23.63660096   22.05043745   23.18398426   18.65335792   26.21785542   23.90922453   31.7331025   17.36904023       AA487893   20.27570383   19.61886578   17.2329505   24.0071361   28.53431361   17.76212522   25.91589986   13.82120963       AA292226   48.60827655   42.38607553   23.18196775   29.16377551   30.35228689   41.74942514   54.37468138   30.13431188       H87106   20.63861132   19.57872959   14.95930266   17.92470933   21.26931436   23.96829793   22.78223035   16.78769736       W96450   23.07654727   21.55396988   24.36095954   28.19647166   31.37132599   30.73388527   31.10677394   21.79020976       N33331   28.36940167   25.78662125   25.65907432   25.90328811   29.92364688   26.88595169   28.03401198   23.03958218       AA405800   25.43365191   26.8056176   19.34783059   23.9119729   29.14739213   20.1376127   20.7481208   15.45920589       T51539   37.04254749   35.58329862   27.22357679   29.39443068   29.26747712   32.85179948   37.13241221   24.37523886       N59764   24.30305591   21.24727999   22.53923803   16.07436069   26.90427658   20.92597239   23.22437469   16.44600319       AA521346   3.927462453   4.483573541   4.871009817   5.009623854   4.220279816   6.412726238   6.63139433   4.36497316       AA428551   7.511216326   7.054388812   6.360096869   6.367864337   4.74719124   11.79753397   11.90853014   8.624379722       AA489383   3.254207402   4.363727468   3.602198976   2.857452654   3.681140068   6.505644423   5.294661541   0.416874791       AA490172   8.563846144   2.818134846   16.5090439   6.336351989   4.552424226   5.084900046   1.540288648   5.753606895       AA504477   20.27058266   23.61171293   22.8431745   20.63453263   28.05090535   20.61037432   19.19136064   15.10292793