Patent Publication Number: US-10308964-B2

Title: Activity of Fe—S cluster requiring proteins

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a continuation of and claims priority to U.S. patent application Ser. No. 13/837,921, filed on Mar. 15, 2013 and U.S. patent application Ser. No. 13/837,893, filed on Mar. 15, 2013 both of which are continuations of and claim priority to U.S. patent application Ser. No. 13/029,558, filed on Feb. 17, 2011, now U.S. Pat. No. 9,297,016, issued on Mar. 29, 2016, which claims the benefit of U.S. Provisional Appl. No. 61/305,333, filed Feb. 17, 2010, each of which is incorporated by reference in its entirety. 
    
    
     SEQUENCE LISTING INFORMATION 
     The content of the electronically submitted sequence listing in ASCII text file (Name: 20161031_CL4842USCNT3_ST25) filed with the application is incorporated herein by reference in its entirety. 
     BACKGROUND OF THE INVENTION 
     Field of the Invention 
     This invention relates generally to the fields of microbiology and biochemistry. Specifically, the present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis activity of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell. 
     Background of the Invention 
     Iron-sulfur (Fe—S) clusters serve as cofactors or prosthetic groups essential for the normal function of the class of proteins that contain them. In the class of Fe—S cluster containing proteins, the Fe—S clusters have been found to play several roles. When proteins of this class are first synthesized by the cell, they lack the Fe—S clusters required for their proper function and are referred to as apoproteins. Fe—S clusters are made in a series of reactions by proteins involved in Fe—S cluster biosynthesis and are transferred to the apo-proteins to form the functional Fe—S cluster containing holoproteins. 
     One such protein that requires Fe—S clusters for proper function is dihydroxy-acid dehydratase (DHAD) (E.C. 4.2.1.9). DHAD catalyzes the conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate, and of 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. The DHAD enzyme is part of naturally occurring biosynthetic pathways producing the branched chain amino acids, (i.e., valine, isoleucine, leucine), and pantothenic acid (vitamin B5). DHAD catalyzed conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate is also a common step in the multiple isobutanol biosynthetic pathways that are disclosed in U.S. Patent Appl. Pub. No. US 20070092957 A1, incorporated by reference herein. Disclosed therein is, e.g., the engineering of recombinant microorganisms for the production of isobutanol. 
     High levels of DHAD activity are desired for increased production of products from biosynthetic pathways that include this enzyme activity, including, e.g., enhanced microbial production of branched chain amino acids, pantothenic acid, and isobutanol. Isobutanol, in particular, is useful as a fuel additive, and its ready availability may reduce the demand for petrochemical fuels. However, since all known DHAD enzymes require a Fe—S cluster for their function, they must be expressed in a host having the genetic machinery to provide the Fe—S clusters required by these proteins. In yeast, mitochondria play an essential role in Fe—S cluster biosynthesis. If the DHAD is to be functionally expressed in yeast cytosol, a system to transport the requisite Fe—S precursor or signal from mitochondria and assemble the Fe—S cluster on the cytosolic apoprotein is required. Prior to the work of the present inventors, it was previously unknown whether yeast could provide Fe—S clusters for any DHAD located in the cytoplasm (since native yeast DHAD is located in the mitochondria) and more importantly when the DHAD is expressed at high levels in the cytoplasm. 
     Under certain conditions the rate of synthesis of Fe—S cluster requiring apo-proteins may exceed the cell&#39;s ability to synthesize and assemble Fe—S clusters for them. Cluster-less apo-proteins that accumulate under these conditions cannot carry out their normal function. Such conditions can include 1) the expression of a heterologous Fe—S cluster requiring protein especially in high amounts, 2) the expression of a native Fe—S cluster biosynthesis protein at higher levels than normal, or 3) a state where the host cell&#39;s ability to synthesize Fe—S clusters is debilitated. 
     BRIEF SUMMARY OF THE INVENTION 
     Disclosed herein is the surprising discovery that recombinant host cells expressing a high level of a heterologous Fe—S cluster requiring protein can supply the complement of Fe—S clusters for that protein if the level(s) of at least one Fe uptake, utilization, and/or Fe—S cluster biosynthesis protein are altered. 
     Provided herein are recombinant host cells comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity wherein said at least one heterologous polynucleotide comprises a high copy number plasmid or a plasmid with a copy number that can be regulated. Also provided are recombinant host cells comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity wherein said at least one heterologous polynucleotide is integrated at least once in the recombinant host cell DNA. Also provided are recombinant host cells comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity, wherein said host cell comprises at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting iron metabolism or Fe—S cluster biosynthesis. Also provided are recombinant host cells comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity and at least one heterologous polynucleotide encoding a polypeptide affecting iron metabolism or Fe—S cluster biosynthesis. 
     In embodiments, said heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of the genes in Tables 7, 8 and 9. In embodiments, said heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of AFT1, AFT2, CCC1, FRA2, and GRX3, and combinations thereof. In embodiments, polypeptide is encoded by a polynucleotide that is constitutive mutant. In embodiments, said constitutive mutant is selected from the group consisting of AFT1 L99A, AFT1 L102A, AFT1 C291F, AFT1 C293F, and combinations thereof. In embodiments said polypeptide affecting Fe—S cluster biosynthesis is encoded by a polynucleotide comprising a high copy number plasmid or a plasmid with a copy number that can be regulated. In embodiments, said polypeptide affecting Fe—S cluster biosynthesis is encoded by a polynucleotide integrated at least once in the recombinant host cell DNA. In embodiments, the at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of CCC1, FRA2, and GRX3, and combinations thereof. In embodiments, the at least one heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of AFT1, AFT2, their mutants, and combinations thereof. 
     In embodiments, said at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity is expressed in multiple copies. In embodiments, said at least one heterologous polynucleotide comprises a high copy number plasmid or a plasmid with a copy number that can be regulated. In embodiments, said at least one heterologous polynucleotide is integrated at least once in the recombinant host cell DNA. In embodiments, said Fe—S cluster biosynthesis is increased compared to a recombinant host cell having endogenous Fe—S cluster biosynthesis. 
     In embodiments, said host cell is a yeast host cell. In embodiments, said yeast host cell is selected from the group consisting of  Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia  and  Pichia.    
     In embodiments, said heterologous polypeptide having dihydroxy-acid dehydratase activity is expressed in the cytosol of the host cell. In embodiments, said heterologous polypeptide having dihydroxy-acid dehydratase activity has an amino acid sequence that matches the Profile HMM of Table 12 with an E value of &lt;10 −5  wherein the polypeptide further comprises all three conserved cysteines, corresponding to positions 56, 129, and 201 in the amino acids sequences of the  Streptococcus mutans  DHAD enzyme corresponding to SEQ ID NO:168. In embodiments, said heterologous polypeptide having dihydroxy-acid dehydratase activity has an amino acid sequence with at least about 90% identity to SEQ ID NO: 168 or SEQ ID NO: 232. In embodiments said polypeptide having dihydroxy-acid dehydratase activity has a specific activity selected from the group consisting of: greater than about 5-fold with respect to the control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity, greater than about 8-fold with respect to the control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity, or greater than about 10-fold with respect to the control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity. In embodiments said polypeptide having dihydroxy-acid dehydratase activity has a specific activity selected from the group consisting of: greater than about 3-fold with respect to a control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity and greater than about 6-fold with respect to the control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity. In embodiments, said polypeptide having dihydroxy-acid dehydratase activity has a specific activity selected from the group consisting of: greater than about 0.25 U/mg; greater than about 0.3 U/mg; greater than about 0.5 U/mg; greater than about 1.0 U/mg; greater than about 1.5 U/mg; greater than about 2.0 U/mg; greater than about 3.0 U/mg; greater than about 4.0 U/mg; greater than about 5.0 U/mg; greater than about 6.0 U/mg; greater than about 7.0 U/mg; greater than about 8.0 U/mg; greater than about 9.0 U/mg; greater than about 10.0 U/mg; greater than about 20.0 U/mg; and greater than about 50.0 U/mg. 
     In embodiments said recombinant host cell produces isobutanol, and in embodiments, said recombinant host cell comprises an isobutanol biosynthetic pathway. 
     Also provided herein are methods of making a product comprising: providing a recombinant host cell; and contacting the recombinant host cell of with a fermentable carbon substrate in a fermentation medium under conditions wherein said product is produced, wherein the product is selected from the group consisting of branched chain amino acids, pantothenic acid, 2-methyl-1-butanol, 3-methyl-1-butanol, isobutanol, and combinations thereof. In embodiments, the methods further comprise optionally recovering said product. In embodiments, the methods further comprise recovering said product. 
     Also provided are methods of making isobutanol comprising: providing a recombinant host cell; contacting the recombinant host cell with a fermentable carbon substrate in a fermentation medium under conditions wherein isobutanol is produced. In embodiments, the methods further comprise optionally recovering said isobutanol. In embodiments, the methods further comprise recovering said isobutanol. 
     Also provided are methods for the conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate comprising: providing a recombinant host cell; growing the recombinant host cell of under conditions where the 2,3-dihydroxyisovalerate is converted to α-ketoisovalerate. In embodiments, the conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate compared to a control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity is increased in an amount selected from the group consisting of: (a) at least about 5%; (b) at least about 10%; (c) at least about 15%; (d) at least about 20%; (e) at least about 25%; (f) at least about 30%; (g) at least about 35%; (h) at least about 40%; (i) at least about 45%; (j) at least about 50%; (k) at least about 60%; (l) at least about 70%; (m) at least about 80%; (n) at least about 90%; and (o) at least about 95%. 
     Also provided are methods for increasing the specific activity of a heterologous polypeptide having dihydroxy-acid dehydratase activity in a recombinant host cell comprising: providing a recombinant host cell; and growing the recombinant host cell of under conditions whereby the heterologous polypeptide having dihydroxy-acid dehydratase activity is expressed in functional form having a specific activity greater than the same host cell lacking said heterologous polypeptide. 
     Also provided are methods for increasing the flux in an Fe—S cluster biosynthesis pathway in a host cell comprising: providing a recombinant host cell; and growing the recombinant host cell under conditions whereby the flux in the Fe—S cluster biosynthesis pathway in the host cell is increased. 
     Also provide are methods of increasing the activity of an Fe—S cluster requiring protein in a recombinant host cell comprising: providing a recombinant host cell comprising an Fe—S cluster requiring protein; changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis in said host cell; and growing the recombinant host cell under conditions whereby the activity of the Fe—S cluster requiring protein is increased. In embodiments, said increase in activity is an amount selected from the group consisting of: greater than about 10%; greater than about 20%; greater than about 30%; greater than about 40%; greater than about 50%; greater than about 60%; greater than about 70%; greater than about 80%; greater than about 90%; and greater than about 95%, 98%, or 99%. In embodiments, the increase in activity is in an amount selected from the group consisting of: greater than about 5-fold; greater than about 8-fold; greater than about 10-fold. In embodiments, the increase in activity is in an amount selected from the group consisting of: greater than about 3-fold and greater than about 6-fold. 
     A method for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell comprising: changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis; measuring the activity of a heterologous Fe—S cluster requiring protein; and comparing the activity of the heterologous Fe—S cluster requiring protein measured in the presence of the change in expression or activity of a polypeptide to the activity of the heterologous Fe—S cluster requiring protein measured in the absence of the change in expression or activity of a polypeptide, wherein an increase in the activity of the heterologous Fe—S cluster requiring protein indicates an increase in the flux in said Fe—S cluster biosynthesis pathway. 
     Provided herein are methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell comprising: changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis; measuring the activity of a polypeptide having dihydroxy-acid dehydratase activity; and comparing the activity of the polypeptide having dihydroxy-acid dehydratase activity measured in the presence of the change to the activity of the polypeptide having dihydroxy-acid dehydratase activity measured in the absence of change, wherein an increase in the activity of the polypeptide having dihydroxy-acid dehydratase activity indicates an increase in the flux in said Fe—S cluster biosynthesis pathway. 
     In embodiments, said changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis comprises deleting, mutating, substituting, expressing, up-regulating, down-regulating, altering the cellular location, altering the state of the protein, and/or adding a cofactor. In embodiments, the Fe—S cluster requiring protein has dihydroxy-acid dehydratase activity and wherein said Fe—S cluster requiring protein having dihydroxy-acid dehydratase activity has an amino acid sequence that matches the Profile HMM of Table 12 with an E value of &lt;10 −5  wherein the polypeptide further comprises all three conserved cysteines, corresponding to positions 56, 129, and 201 in the amino acids sequences of the  Streptococcus mutans  DHAD enzyme corresponding to SEQ ID NO:168. In embodiments, the polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of the genes in Tables 7, 8 and 9. 
     Also provided are recombinant host cells comprising at least one polynucleotide encoding a polypeptide identified by the methods provided herein. In embodiments, said host cell further comprises at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity. In embodiments, said heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity is expressed in multiple copies. In embodiments, said heterologous polynucleotide comprises a high copy number plasmid or a plasmid with a copy number that can be regulated. In embodiments, said heterologous polynucleotide is integrated at least once in the recombinant host cell DNA. 
     In embodiments, said host cell is a yeast host cell. In embodiments, said yeast host cell is selected from the group consisting of  Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia , and  Pichia . In embodiments, said heterologous polypeptide having dihydroxy-acid dehydratase activity is expressed in the cytosol of the host cell. In embodiments, said heterologous polypeptide having dihydroxy-acid dehydratase activity has an amino acid sequence that matches the Profile HMM of Table 12 with an E value of &lt;10 −5  wherein the polypeptide further comprises all three conserved cysteines, corresponding to positions 56, 129, and 201 in the amino acids sequences of the  Streptococcus mutans  DHAD enzyme corresponding to SEQ ID NO:168. In embodiments, said recombinant host cell produces a product selected from the group consisting of branched chain amino acids, pantothenic acid, 2-methyl-1-butanol, 3-methyl-1-butanol, isobutanol, and combinations thereof. In embodiments, recombinant host cell produces isobutanol. In embodiments, said recombinant host cell comprises an isobutanol biosynthetic pathway. In embodiments said isobutanol biosynthetic pathway comprises at least one polypeptide encoded by a polynucleotide heterologous to the host cell. In embodiments, said isobutanol biosynthetic pathway comprises at least two polypeptides encoded by polynucleotides heterologous to the host cell. 
     In embodiments, monomers of the polypeptides of the invention having dihydroxy-acid dehydratase activity have an Fe—S cluster loading selected from the group consisting of: (a) at least about 10%; (b) at least about 15%; (c) at least about 20%; (d) at least about 25%; (e) at least about 30%; (f) at least about 35%; (g) at least about 40%; (h) at least about 45%; (i) at least about 50%; (j) at least about 60%; (k) at least about 70%; (l) at least about 80%; (m) at least about 90%; and (n) at least about 95%. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS/FIGURES 
         FIG. 1A  depicts a vector map of a vector for overexpression of the IlvD gene from  S. mutans.    
         FIG. 1B  depicts a vector map of an integration vector for overexpression of the IlvD gene from  S. mutans  in the chromosome. 
         FIG. 2  depicts a vector map of a centromere vector used to clone AFT1 or AFT1 mutants and useful for other genes of interest. 
         FIG. 3  depicts a UV-Vis absorbance spectrum of purified  S. mutans  DHAD. 
         FIG. 4  depicts an EPR spectrum of purified  S. mutans  DHAD. 
         FIG. 5  depicts a biosynthetic pathway for biosynthesis of isobutanol. 
         FIG. 6A  depicts a schematic of  Azotobacter vinelandii  nif genes. 
         FIG. 6B  depicts a schematic of additional  Azotobacter vinelandii  nif genes. 
         FIG. 6C  depicts a schematic of the equation in which NFU acts as a persulfide reductase. 
         FIG. 7  depicts a schematic of  Helicobacter pylori  nif genes. 
         FIG. 8  depicts a schematic of  E. coli  isc genes. 
         FIG. 9  depicts a schematic of  E. coli  suf genes. 
         FIG. 10  depicts a schematic of the cytosolic [2Fe-2S] biosynthesis and assembly system. 
         FIG. 11  depicts a vector map of a vector for overexpression of the IlvD gene from  L. lactis.    
     
    
    
     Table 12 is a table of the Profile HMM for dihydroxy-acid dehydratases based on enzymes with assayed function prepared as described in U.S. patent application Ser. No. 12/569,636, filed Sep. 29, 2009. Table 12 is submitted herewith electronically and is incorporated herein by reference. 
     DETAILED DESCRIPTION OF THE INVENTION 
     Described herein is a method to increase the fraction of the Fe—S cluster requiring proteins that are loaded with Fe—S clusters. Also described are recombinant host cells that express functional Fe—S cluster requiring proteins, such as DHAD enzymes, and at least one heterologous Fe uptake, utilization, or Fe—S cluster biosynthesis protein, recombinant host cells that express functional DHAD enzymes and comprise at least one deletion, mutation, and/or substitution in a native protein involved in Fe utilization or Fe—S cluster biosynthesis, or recombinant host cells comprising combinations thereof. In addition, the present invention describes a method to identify polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell. Also described is a method to identify polypeptides that alter the activity of an Fe—S cluster requiring protein. 
     Definitions 
     Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present application including the definitions will control. Also, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular. All publications, patents and other references mentioned herein are incorporated by reference in their entireties for all purposes. 
     In order to further define this invention, the following terms and definitions are herein provided. 
     As used herein, the terms “comprises,” “comprising,” “includes,” “including,” “has,” “having,” “contains” or “containing,” or any other variation thereof, will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers. For example, a composition, a mixture, a process, a method, an article, or an apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, mixture, process, method, article, or apparatus. Further, unless expressly stated to the contrary, “or” refers to an inclusive or and not to an exclusive or. For example, a condition A or B is satisfied by any one of the following: A is true (or present) and B is false (or not present), A is false (or not present) and B is true (or present), and both A and B are true (or present). 
     As used herein, the term “consists of” or variations such as “consist of” or “consisting of,” as used throughout the specification and claims, indicate the inclusion of any recited integer or group of integers, but that no additional integer or group of integers may be added to the specified method, structure, or composition. 
     As used herein, the term “consists essentially of,” or variations such as “consist essentially of” or “consisting essentially of,” as used throughout the specification and claims, indicate the inclusion of any recited integer or group of integers, and the optional inclusion of any recited integer or group of integers that do not materially change the basic or novel properties of the specified method, structure or composition. See M.P.E.P. § 2111.03. 
     Also, the indefinite articles “a” and “an” preceding an element or component of the invention are intended to be nonrestrictive regarding the number of instances, i.e., occurrences of the element or component. Therefore “a” or “an” should be read to include one or at least one, and the singular word form of the element or component also includes the plural unless the number is obviously meant to be singular. 
     The term “invention” or “present invention” as used herein is a non-limiting term and is not intended to refer to any single embodiment of the particular invention but encompasses all possible embodiments as described in the application. 
     As used herein, the term “about” modifying the quantity of an ingredient or reactant of the invention employed refers to variation in the numerical quantity that can occur, for example, through typical measuring and liquid handling procedures used for making concentrates or solutions in the real world; through inadvertent error in these procedures; through differences in the manufacture, source, or purity of the ingredients employed to make the compositions or to carry out the methods; and the like. The term “about” also encompasses amounts that differ due to different equilibrium conditions for a composition resulting from a particular initial mixture. Whether or not modified by the term “about”, the claims include equivalents to the quantities. In one embodiment, the term “about” means within 10% of the reported numerical value, preferably within 5% of the reported numerical value. 
     The term “isobutanol biosynthetic pathway” refers to an enzyme pathway to produce isobutanol from pyruvate. 
     The term “a facultative anaerobe” refers to a microorganism that can grow in both aerobic and anaerobic environments. 
     The term “carbon substrate” or “fermentable carbon substrate” refers to a carbon source capable of being metabolized by host organisms of the present invention and particularly carbon sources selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and one-carbon substrates or mixtures thereof. 
     The term “Fe—S cluster biosynthesis” refers to biosynthesis of Fe—S clusters, including, e.g., the assembly and loading of Fe—S clusters. The term “Fe—S cluster biosynthesis genes”, “Fe—S cluster biosynthesis proteins” or “Fe—S cluster biosynthesis pathway” refers to those polynucleotides/genes and the encoded polypeptides that are involved in the biosynthesis of Fe—S clusters, including, e.g., the assembly and loading of Fe—S clusters. 
     The term “Fe uptake and utilization” refers to processes which can effect Fe—S cluster biosynthesis such as Fe sensing, uptake, utilization, and homeostasis. “Fe uptake and utilization genes” refers to those polynucleotides/genes and the encoded polypeptides that are involved in Fe uptake, utilization, and homeostasis. Some of these polynucleotides/genes are contained in the “Fe Regulon” that has been described in the literature and is further described hereafter. As used herein, Fe uptake and utilization genes and Fe—S cluster biosynthesis genes can encode a polypeptide affecting Fe—S cluster biosynthesis. 
     The term “specific activity” as used herein is defined as the units of activity in a given amount of protein. Thus, the specific activity is not directly measured but is calculated by dividing 1) the activity in units/ml of the enzyme sample by 2) the concentration of protein in that sample, so the specific activity is expressed as units/mg. The specific activity of a sample of pure, fully active enzyme is a characteristic of that enzyme. The specific activity of a sample of a mixture of proteins is a measure of the relative fraction of protein in that sample that is composed of the active enzyme of interest. The specific activity of a polypeptide of the invention may be selected from greater than about 0.25 U/mg; greater than about 0.3 U/mg; greater than about 0.4 U/mg; greater than about 0.5 U/mg; greater than about 0.6 U/mg; greater than about 0.7 U/mg; greater than about 0.8 U/mg; greater than about 0.9 U/mg; greater than about 1.0 U/mg; greater than about 1.5 U/mg; greater than about 2.0 U/mg; greater than about 2.5 U/mg; greater than about 3.0 U/mg; greater than about 3.5 U/mg; greater than about 4.0 U/mg; greater than about 5.5 U/mg; greater than about 5.0 U/mg; greater than about 6.0 U/mg; greater than about 6.5 U/mg; greater than about 7.0 U/mg; greater than about 7.5 U/mg; greater than about 8.0 U/mg; greater than about 8.5 U/mg; greater than about 9.0 U/mg; greater than about 9.5 U/mg; greater than about 10.0 U/mg; greater than about 20.0 U/mg; or greater than about 50.0 U/mg. In one embodiment, the specific activity of a polypeptide of the invention is greater than about 0.25 U/mg. In another embodiment, the specific activity is greater than about 1.0 U/mg. In yet another embodiment, the specific activity is greater than about 2.0 U/mg or greater than about 3.0 U/mg. 
     The term “polynucleotide” is intended to encompass a singular nucleic acid as well as plural nucleic acids, and refers to a nucleic acid molecule or construct, e.g., messenger RNA (mRNA) or plasmid DNA (pDNA). A polynucleotide can contain the nucleotide sequence of the full-length cDNA sequence, or a fragment thereof, including the untranslated 5′ and 3′ sequences and the coding sequences. The polynucleotide can be composed of any polyribonucleotide or polydeoxyribonucleotide, which may be unmodified RNA or DNA or modified RNA or DNA. For example, polynucleotides can be composed of single- and double-stranded DNA, DNA that is a mixture of single- and double-stranded regions, single- and double-stranded RNA, and RNA that is mixture of single- and double-stranded regions, hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded or a mixture of single- and double-stranded regions. “Polynucleotide” embraces chemically, enzymatically, or metabolically modified forms. 
     A polynucleotide sequence may be referred to as “isolated,” in which it has been removed from its native environment. For example, a heterologous polynucleotide encoding a polypeptide or polypeptide fragment having dihydroxy-acid dehydratase activity contained in a vector is considered isolated for the purposes of the present invention. Further examples of an isolated polynucleotide include recombinant polynucleotides maintained in heterologous host cells or purified (partially or substantially) polynucleotides in solution. Isolated polynucleotides or nucleic acids according to the present invention further include such molecules produced synthetically. An isolated polynucleotide fragment in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA or synthetic DNA. 
     The term “gene” refers to a polynucleotide that is capable of being expressed as a specific protein, optionally including regulatory sequences preceding (5′ non-coding sequences) and following (3′ non-coding sequences) the coding sequence. “Native gene” refers to a gene as found in nature with its own regulatory sequences. “Chimeric gene” refers to any gene that is not a native gene, comprising regulatory and coding sequences that are not found together in nature. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. 
     As used herein, a “coding region” is a portion of nucleic acid which consists of codons translated into amino acids. Although a “stop codon” (TAG, TGA, or TAA) is not translated into an amino acid, it may be considered to be part of a coding region, but any flanking sequences, for example promoters, ribosome binding sites, transcriptional terminators, introns, and the like, are not part of a coding region. Two or more coding regions of the present invention can be present in a single polynucleotide construct, e.g., on a single vector, or in separate polynucleotide constructs, e.g., on separate (different) vectors. Furthermore, any vector may contain a single coding region, or may comprise two or more coding regions. In addition, a vector, polynucleotide, or nucleic acid of the invention may encode heterologous coding regions. 
     The term “endogenous,” when used in reference to a polynucleotide, a gene, or a polypeptide refers to a native polynucleotide or gene in its natural location in the genome of an organism, or for a native polypeptide, is transcribed and translated from this location in the genome. 
     The term “heterologous” when used in reference to a polynucleotide, a gene, or a polypeptide refers to a polynucleotide, gene, or polypeptide not normally found in the host organism. “Heterologous” also includes a native coding region, or portion thereof, that is reintroduced into the source organism in a form that is different from the corresponding native gene, e.g., not in its natural location in the organism&#39;s genome. The heterologous polynucleotide or gene may be introduced into the host organism by, e.g., gene transfer. A heterologous gene may include a native coding region with non-native regulatory regions that is reintroduced into the native host. A “transgene” is a gene that has been introduced into the genome by a transformation procedure. 
     The term “recombinant genetic expression element” refers to a nucleic acid fragment that expresses one or more specific proteins, including regulatory sequences preceding (5′ non-coding sequences) and following (3′ termination sequences) coding sequences for the proteins. A chimeric gene is a recombinant genetic expression element. The coding regions of an operon may form a recombinant genetic expression element, along with an operably linked promoter and termination region. 
     “Regulatory sequences” refers to nucleotide sequences located upstream (5′ non-coding sequences), within, or downstream (3′ non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences may include promoters, enhancers, operators, repressors, transcription termination signals, translation leader sequences, introns, polyadenylation recognition sequences, RNA processing site, effector binding site and stem-loop structure. 
     The term “promoter” refers to a nucleic acid sequence capable of controlling the expression of a coding sequence or functional RNA. In general, a coding sequence is located 3′ to a promoter sequence. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even comprise synthetic nucleic acid segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental or physiological conditions. Promoters which cause a gene to be expressed in most cell types at most times are commonly referred to as “constitutive promoters”. “Inducible promoters,” on the other hand, cause a gene to be expressed when the promoter is induced or turned on by a promoter-specific signal or molecule. It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of different lengths may have identical promoter activity. 
     The term “operably linked” refers to the association of nucleic acid sequences on a single nucleic acid fragment so that the function of one is affected by the other. For example, a promoter is operably linked with a coding sequence when it is capable of effecting the expression of that coding sequence (i.e., that the coding sequence is under the transcriptional control of the promoter). Coding sequences can be operably linked to regulatory sequences in sense or antisense orientation. 
     The term “expression”, as used herein, refers to the transcription and accumulation of sense (mRNA) or antisense RNA derived from the nucleic acid fragment of the invention. Expression may also refer to translation of mRNA into a polypeptide. The process includes any manifestation of the functional presence of the expressed polynucleotide, gene, or polypeptide within the cell including, without limitation, gene knockdown as well as both transient expression and stable expression. 
     The term “over-expression”, as used herein, refers to expression that is higher than endogenous expression of the same or related polynucleotide or gene. A heterologous polynucleotide or gene is also over-expressed if its expression is higher than that of a comparable endogenous gene, or if its expression is higher than that of the same polynucleotide or gene introduced by a means that does not overexpress the polynucleotide or gene. For example, a polynucleotide can be expressed in a host cell from a low copy number plasmid, which is present in only limited or few copies, and the same polynucleotide can be over-expressed in a host cell from a high copy number plasmid or a plasmid with a copy number that can be regulated, which is present in multiple copies. Any means can be used to over-express a polynucleotide, so long as it increases the copies of the polynucleotide in the host cell. In addition to using a high copy number plasmid, or a plasmid with a copy number that can be regulated, a polynucleotide can be over-expressed by multiple chromosomal integrations. 
     Expression or over-expression of a polypeptide of the invention in a recombinant host cell can be quantified according to any number of methods known to the skilled artisan and can be represented, e.g., by a percent of total cell protein. The percent of total protein can be an amount selected from greater than about 0.001% of total cell protein; greater than about 0.01% of total cell protein; greater than about 0.1% of total cell protein; greater than about 0.5% of total cell protein; greater than about 1.0% of total cell protein; greater than about 2.0% of total cell protein; greater than about 3% of total cell protein; greater than about 4.0% of total cell protein; greater than about 5% of total cell protein; greater than about 6.0% of total cell protein; greater than about 7.0% of total cell protein; greater than about 8.0% of total cell protein; greater than about 9.0% of total cell protein; greater than about 10% of total cell protein; or greater than about 20% of total cell protein. In one embodiment, the amount of polypeptide expressed is greater that about 0.5% of total cell protein. In another embodiment, the amount of polypeptide expressed is greater than about 1.0% of total cell protein or greater than about 2.0% of total cell protein. 
     As used herein the term “transformation” refers to the transfer of a nucleic acid fragment into a host organism, resulting in genetically stable inheritance with or without selections. Host organisms containing the transformed nucleic acid fragments are referred to as “transgenic” or “recombinant” or “transformed” organisms. 
     The terms “plasmid” and “vector” as used herein, refer to an extra chromosomal element often carrying genes which are not part of the central metabolism of the cell, and usually in the form of circular double-stranded DNA molecules. Such elements may be autonomously replicating sequences, genome integrating sequences, phage or nucleotide sequences, linear or circular, of a single- or double-stranded DNA or RNA, derived from any source, in which a number of nucleotide sequences have been joined or recombined into a unique construction which is capable of introducing a promoter fragment and DNA sequence for a selected gene product along with appropriate 3′ untranslated sequence into a cell. 
     As used herein the term “codon degeneracy” refers to the nature in the genetic code permitting variation of the nucleotide sequence without effecting the amino acid sequence of an encoded polypeptide. The skilled artisan is well aware of the “codon-bias” exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a gene for improved expression in a host cell, it is desirable to design the gene such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell. 
     The term “codon-optimized” as it refers to genes or coding regions of nucleic acid molecules for transformation of various hosts, refers to the alteration of codons in the gene or coding regions of the nucleic acid molecules to reflect the typical codon usage of the host organism without altering the polypeptide encoded by the DNA. Such optimization includes replacing at least one, or more than one, or a significant number, of codons with one or more codons that are more frequently used in the genes of that organism. 
     Deviations in the nucleotide sequence that comprise the codons encoding the amino acids of any polypeptide chain allow for variations in the sequence coding for the gene. Since each codon consists of three nucleotides, and the nucleotides comprising DNA are restricted to four specific bases, there are 64 possible combinations of nucleotides, 61 of which encode amino acids (the remaining three codons encode signals ending translation). The “genetic code” which shows which codons encode which amino acids is reproduced herein as Table 1. As a result, many amino acids are designated by more than one codon. For example, the amino acids alanine and proline are coded for by four triplets, serine and arginine by six, whereas tryptophan and methionine are coded by just one triplet. This degeneracy allows for DNA base composition to vary over a wide range without altering the amino acid sequence of the proteins encoded by the DNA. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 The Standard Genetic Code 
               
            
           
           
               
               
               
               
               
            
               
                   
                 T 
                 C 
                 A 
                 G 
               
               
                   
               
               
                 T 
                 TTT Phe (F) 
                 TCT Ser (S) 
                 TAT Tyr (Y) 
                 TGT Cys (C) 
               
               
                   
                 TTC″ 
                 TCC″ 
                 TAC″ 
                 TGC 
               
               
                   
                 TTA Leu (L) 
                 TCA″ 
                 TAA Stop 
                 TGA Stop 
               
               
                   
                 TTG″ 
                 TCG″ 
                 TAG Stop 
                 TGG Trp (W) 
               
               
                   
               
               
                 C 
                 CTT Leu (L) 
                 CCT Pro (P) 
                 CAT His (H) 
                 CGT Arg (R) 
               
               
                   
                 CTC″ 
                 CCC″ 
                 CAC″ 
                 CGC″ 
               
               
                   
                 CTA″ 
                 CCA″ 
                 CAA Gln (Q) 
                 CGA″ 
               
               
                   
                 CTG″ 
                 CCG″ 
                 CAG″ 
                 CGG″ 
               
               
                   
               
               
                 A 
                 ATT Ile (I) 
                 ACT Thr (T) 
                 AAT Asn (N) 
                 AGT Ser (S) 
               
               
                   
                 ATC″ 
                 ACC″ 
                 AAC″ 
                 AGC″ 
               
               
                   
                 ATA″ 
                 ACA″ 
                 AAA Lys (K) 
                 AGA Arg (R) 
               
               
                   
                   ATG  Met 
                 ACG″ 
                 AAG″ 
                 AGG″ 
               
               
                   
                 (M) 
                   
                   
                   
               
               
                   
               
               
                 G 
                 GTT Val (V) 
                 GCT Ala (A) 
                 GAT Asp (D) 
                 GGT Gly (G) 
               
               
                   
                 GTC″ 
                 GCC″ 
                 GAC″ 
                 GGC″ 
               
               
                   
                 GTA″ 
                 GCA″ 
                 GAA Glu (E) 
                 GGA″ 
               
               
                   
                 GTG″ 
                 GCG″ 
                 GAG″ 
                 GGG″ 
               
               
                   
               
            
           
         
       
     
     Many organisms display a bias for use of particular codons to code for insertion of a particular amino acid in a growing peptide chain. Codon preference, or codon bias, differences in codon usage between organisms, is afforded by degeneracy of the genetic code, and is well documented among many organisms. Codon bias often correlates with the efficiency of translation of messenger RNA (mRNA), which is in turn believed to be dependent on, inter alia, the properties of the codons being translated and the availability of particular transfer RNA (tRNA) molecules. The predominance of selected tRNAs in a cell is generally a reflection of the codons used most frequently in peptide synthesis. Accordingly, genes can be tailored for optimal gene expression in a given organism based on codon optimization. 
     Given the large number of gene sequences available for a wide variety of animal, plant and microbial species, it is possible to calculate the relative frequencies of codon usage. Codon usage tables are readily available, for example, at the “Codon Usage Database” available at the Kazusa DNA Research Institute, Japan, and these tables can be adapted in a number of ways. See Nakamura, Y., et al.  Nucl. Acids Res.  28:292 (2000). Codon usage tables for yeast, calculated from GenBank Release 128.0 [15 Feb. 2002], are reproduced below as Table 2. This table uses mRNA nomenclature, and so instead of thymine (T) which is found in DNA, the tables use uracil (U) which is found in RNA. Table 2 has been adapted so that frequencies are calculated for each amino acid, rather than for all 64 codons. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Codon Usage Table for  Saccharomyces cerevisiae  Genes 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Frequency per 
               
               
                   
                 Amino Acid 
                 Codon 
                 Number 
                 thousand 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 Phe 
                 UUU 
                 170666 
                 26.1 
               
               
                   
                 Phe 
                 UUC 
                 120510 
                 18.4 
               
               
                   
                 Leu 
                 UUA 
                 170884 
                 26.2 
               
               
                   
                 Leu 
                 UUG 
                 177573 
                 27.2 
               
               
                   
                 Leu 
                 CUU 
                 80076 
                 12.3 
               
               
                   
                 Leu 
                 CUC 
                 35545 
                 5.4 
               
               
                   
                 Leu 
                 CUA 
                 87619 
                 13.4 
               
               
                   
                 Leu 
                 CUG 
                 68494 
                 10.5 
               
               
                   
                 Ile 
                 AUU 
                 196893 
                 30.1 
               
               
                   
                 Ile 
                 AUC 
                 112176 
                 17.2 
               
               
                   
                 Ile 
                 AUA 
                 116254 
                 17.8 
               
               
                   
                 Met 
                 AUG 
                 136805 
                 20.9 
               
               
                   
                 Val 
                 GUU 
                 144243 
                 22.1 
               
               
                   
                 Val 
                 GUC 
                 76947 
                 11.8 
               
               
                   
                 Val 
                 GUA 
                 76927 
                 11.8 
               
               
                   
                 Val 
                 GUG 
                 70337 
                 10.8 
               
               
                   
                 Ser 
                 UCU 
                 153557 
                 23.5 
               
               
                   
                 Ser 
                 UCC 
                 92923 
                 14.2 
               
               
                   
                 Ser 
                 UCA 
                 122028 
                 18.7 
               
               
                   
                 Ser 
                 UCG 
                 55951 
                 8.6 
               
               
                   
                 Ser 
                 AGU 
                 92466 
                 14.2 
               
               
                   
                 Ser 
                 AGC 
                 63726 
                 9.8 
               
               
                   
                 Pro 
                 CCU 
                 88263 
                 13.5 
               
               
                   
                 Pro 
                 CCC 
                 44309 
                 6.8 
               
               
                   
                 Pro 
                 CCA 
                 119641 
                 18.3 
               
               
                   
                 Pro 
                 CCG 
                 34597 
                 5.3 
               
               
                   
                 Thr 
                 ACU 
                 132522 
                 20.3 
               
               
                   
                 Thr 
                 ACC 
                 83207 
                 12.7 
               
               
                   
                 Thr 
                 ACA 
                 116084 
                 17.8 
               
               
                   
                 Thr 
                 ACG 
                 52045 
                 8.0 
               
               
                   
                 Ala 
                 GCU 
                 138358 
                 21.2 
               
               
                   
                 Ala 
                 GCC 
                 82357 
                 12.6 
               
               
                   
                 Ala 
                 GCA 
                 105910 
                 16.2 
               
               
                   
                 Ala 
                 GCG 
                 40358 
                 6.2 
               
               
                   
                 Tyr 
                 UAU 
                 122728 
                 18.8 
               
               
                   
                 Tyr 
                 UAC 
                 96596 
                 14.8 
               
               
                   
                 His 
                 CAU 
                 89007 
                 13.6 
               
               
                   
                 His 
                 CAC 
                 50785 
                 7.8 
               
               
                   
                 Gln 
                 CAA 
                 178251 
                 27.3 
               
               
                   
                 Gln 
                 CAG 
                 79121 
                 12.1 
               
               
                   
                 Asn 
                 AAU 
                 233124 
                 35.7 
               
               
                   
                 Asn 
                 AAC 
                 162199 
                 24.8 
               
               
                   
                 Lys 
                 AAA 
                 273618 
                 41.9 
               
               
                   
                 Lys 
                 AAG 
                 201361 
                 30.8 
               
               
                   
                 Asp 
                 GAU 
                 245641 
                 37.6 
               
               
                   
                 Asp 
                 GAC 
                 132048 
                 20.2 
               
               
                   
                 Glu 
                 GAA 
                 297944 
                 45.6 
               
               
                   
                 Glu 
                 GAG 
                 125717 
                 19.2 
               
               
                   
                 Cys 
                 UGU 
                 52903 
                 8.1 
               
               
                   
                 Cys 
                 UGC 
                 31095 
                 4.8 
               
               
                   
                 Trp 
                 UGG 
                 67789 
                 10.4 
               
               
                   
                 Arg 
                 CGU 
                 41791 
                 6.4 
               
               
                   
                 Arg 
                 CGC 
                 16993 
                 2.6 
               
               
                   
                 Arg 
                 CGA 
                 19562 
                 3.0 
               
               
                   
                 Arg 
                 CGG 
                 11351 
                 1.7 
               
               
                   
                 Arg 
                 AGA 
                 139081 
                 21.3 
               
               
                   
                 Arg 
                 AGG 
                 60289 
                 9.2 
               
               
                   
                 Gly 
                 GGU 
                 156109 
                 23.9 
               
               
                   
                 Gly 
                 GGC 
                 63903 
                 9.8 
               
               
                   
                 Gly 
                 GGA 
                 71216 
                 10.9 
               
               
                   
                 Gly 
                 GGG 
                 39359 
                 6.0 
               
               
                   
                 Stop 
                 UAA 
                 6913 
                 1.1 
               
               
                   
                 Stop 
                 UAG 
                 3312 
                 0.5 
               
               
                   
                 Stop 
                 UGA 
                 4447 
                 0.7 
               
               
                   
                   
               
            
           
         
       
     
     By utilizing this or similar tables, one of ordinary skill in the art can apply the frequencies to any given polypeptide sequence, and produce a nucleic acid fragment of a codon-optimized coding region which encodes the polypeptide, but which uses codons optimal for a given species. 
     Randomly assigning codons at an optimized frequency to encode a given polypeptide sequence, can be done manually by calculating codon frequencies for each amino acid, and then assigning the codons to the polypeptide sequence randomly. Additionally, various algorithms and computer software programs are readily available to those of ordinary skill in the art. For example, the “EditSeq” function in the Lasergene Package, available from DNAstar, Inc., Madison, Wis., the backtranslation function in the VectorNTI Suite, available from InforMax, Inc., Bethesda, Md., and the “backtranslate” function in the GCG-Wisconsin Package, available from Accelrys, Inc., San Diego, Calif. In addition, various resources are publicly available to codon-optimize coding region sequences, e.g., the “backtranslation” function (Entelechon GmbH, Regensburg, Germany) and the “backtranseq” function (NRC Saskatoon Bioinformatics, Saskatoon, Saskatchewan, Canada). Constructing a rudimentary algorithm to assign codons based on a given frequency can also easily be accomplished with basic mathematical functions by one of ordinary skill in the art. 
     Codon-optimized coding regions can be designed by various methods known to those skilled in the art including software packages such as “synthetic gene designer” (University of Maryland, Baltimore, Md.). 
     As used herein, the term “polypeptide” is intended to encompass a singular “polypeptide” as well as plural “polypeptides,” and refers to a molecule composed of monomers (amino acids) linearly linked by amide bonds (also known as peptide bonds). The term “polypeptide” refers to any chain or chains of two or more amino acids, and does not refer to a specific length of the product. Thus, peptides, dipeptides, tripeptides, oligopeptides, “protein,” “amino acid chain,” or any other term used to refer to a chain or chains of two or more amino acids, are included within the definition of “polypeptide,” and the term “polypeptide” may be used instead of, or interchangeably with any of these terms. A polypeptide may be derived from a natural biological source or produced by recombinant technology, but is not necessarily translated from a designated nucleic acid sequence. It may be generated in any manner, including by chemical synthesis. 
     By an “isolated” polypeptide or a fragment, variant, or derivative thereof is intended a polypeptide that is not in its natural milieu. No particular level of purification is required. For example, an isolated polypeptide can be removed from its native or natural environment. Recombinantly produced polypeptides and proteins expressed in host cells are considered isolated for purposed of the invention, as are native or recombinant polypeptides which have been separated, fractionated, or partially or substantially purified by any suitable technique. 
     As used herein, the term “variant” refers to a polypeptide differing from a specifically recited polypeptide of the invention, such as DHAD, by amino acid insertions, deletions, mutations, and substitutions, created using, e.g., recombinant DNA techniques, such as mutagenesis. Guidance in determining which amino acid residues may be replaced, added, or deleted without abolishing activities of interest, may be found by comparing the sequence of the particular polypeptide with that of homologous polypeptides, e.g., yeast or bacterial, and minimizing the number of amino acid sequence changes made in regions of high homology (conserved regions) or by replacing amino acids with consensus sequences. 
     Alternatively, recombinant polynucleotide variants encoding these same or similar polypeptides may be synthesized or selected by making use of the “redundancy” in the genetic code. Various codon substitutions, such as silent changes which produce various restriction sites, may be introduced to optimize cloning into a plasmid or viral vector for expression. Mutations in the polynucleotide sequence may be reflected in the polypeptide or domains of other peptides added to the polypeptide to modify the properties of any part of the polypeptide. For example, mutations can be used to reduce or eliminate expression of a target protein and include, but are not limited to, deletion of the entire gene or a portion of the gene, inserting a DNA fragment into the gene (in either the promoter or coding region) so that the protein is not expressed or expressed at lower levels, introducing a mutation into the coding region which adds a stop codon or frame shift such that a functional protein is not expressed, and introducing one or more mutations into the coding region to alter amino acids so that a non-functional or a less enzymatically active protein is expressed. 
     Amino acid “substitutions” may be the result of replacing one amino acid with another amino acid having similar structural and/or chemical properties, i.e., conservative amino acid replacements, or they may be the result of replacing one amino acid with an amino acid having different structural and/or chemical properties, i.e., non-conservative amino acid replacements. “Conservative” amino acid substitutions may be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, or the amphipathic nature of the residues involved. For example, nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; positively charged (basic) amino acids include arginine, lysine, and histidine; and negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Alternatively, “non-conservative” amino acid substitutions may be made by selecting the differences in polarity, charge, solubility, hydrophobicity, hydrophilicity, or the amphipathic nature of any of these amino acids. “Insertions” or “deletions” may be within the range of variation as structurally or functionally tolerated by the recombinant proteins. The variation allowed may be experimentally determined by systematically making insertions, deletions, or substitutions of amino acids in a polypeptide molecule using recombinant DNA techniques and assaying the resulting recombinant variants for activity. 
     A “substantial portion” of an amino acid or nucleotide sequence is that portion comprising enough of the amino acid sequence of a polypeptide or the nucleotide sequence of a gene to putatively identify that polypeptide or gene, either by manual evaluation of the sequence by one skilled in the art, or by computer-automated sequence comparison and identification using algorithms such as BLAST (Altschul, S. F., et al.,  J. Mol. Biol.,  215:403-410 (1993)). In general, a sequence of ten or more contiguous amino acids or thirty or more nucleotides is necessary in order to putatively identify a polypeptide or nucleic acid sequence as homologous to a known protein or gene. Moreover, with respect to nucleotide sequences, gene specific oligonucleotide probes comprising 20-30 contiguous nucleotides may be used in sequence-dependent methods of gene identification (e.g., Southern hybridization) and isolation (e.g., in situ hybridization of bacterial colonies or bacteriophage plaques). In addition, short oligonucleotides of 12-15 bases may be used as amplification primers in PCR in order to obtain a particular nucleic acid fragment comprising the primers. Accordingly, a “substantial portion” of a nucleotide sequence comprises enough of the sequence to specifically identify and/or isolate a nucleic acid fragment comprising the sequence. The instant specification teaches the complete amino acid and nucleotide sequence encoding particular proteins. The skilled artisan, having the benefit of the sequences as reported herein, may now use all or a substantial portion of the disclosed sequences for purposes known to those skilled in this art. Accordingly, the instant invention comprises the complete sequences as reported in the accompanying Sequence Listing, as well as substantial portions of those sequences as defined above. 
     The term “complementary” is used to describe the relationship between nucleotide bases that are capable of hybridizing to one another. For example, with respect to DNA, adenine is complementary to thymine and cytosine is complementary to guanine, and with respect to RNA, adenine is complementary to uracil and cytosine is complementary to guanine. 
     The term “percent identity”, as known in the art, is a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, as determined by comparing the sequences. In the art, “identity” also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings of such sequences. “Identity” and “similarity” can be readily calculated by known methods, including but not limited to those described in: 1.)  Computational Molecular Biology  (Lesk, A. M., Ed.) Oxford University: NY (1988); 2.)  Biocomputing: Informatics and Genome Projects  (Smith, D. W., Ed.) Academic: NY (1993); 3.)  Computer Analysis of Sequence Data, Part I  (Griffin, A. M., and Griffin, H. G., Eds.) Humania: NJ (1994); 4.)  Sequence Analysis in Molecular Biology  (von Heinje, G., Ed.) Academic (1987); and 5.)  Sequence Analysis Primer  (Gribskov, M. and Devereux, J., Eds.) Stockton: NY (1991). 
     Preferred methods to determine identity are designed to give the best match between the sequences tested. Methods to determine identity and similarity are codified in publicly available computer programs. Sequence alignments and percent identity calculations may be performed using the MegAlign™ program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiple alignments of the sequences is performed using the “Clustal method of alignment” which encompasses several varieties of the algorithm including the “Clustal V method of alignment” corresponding to the alignment method labeled Clustal V (described by Higgins and Sharp,  CABIOS.  5:151-153 (1989); Higgins, D. G. et al.,  Comput. Appl. Biosci.,  8:189-191 (1992)) and found in the MegAlign™ program of the LASERGENE bioinformatics computing suite (DNASTAR Inc.). For multiple alignments, the default values correspond to GAP PENALTY=10 and GAP LENGTH PENALTY=10. Default parameters for pairwise alignments and calculation of percent identity of protein sequences using the Clustal method are KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. For nucleic acids these parameters are KTUPLE=2, GAP PENALTY=5, WINDOW=4 and DIAGONALS SAVED=4. After alignment of the sequences using the Clustal V program, it is possible to obtain a “percent identity” by viewing the “sequence distances” table in the same program. Additionally the “Clustal W method of alignment” is available and corresponds to the alignment method labeled Clustal W (described by Higgins and Sharp,  CABIOS.  5:151-153 (1989); Higgins, D. G. et al.,  Comput. Appl. Biosci.  8:189-191(1992)) and found in the MegAlign™ v6.1 program of the LASERGENE bioinformatics computing suite (DNASTAR Inc.). Default parameters for multiple alignment (GAP PENALTY=10, GAP LENGTH PENALTY=0.2, Delay Divergen Seqs(%)=30, DNA Transition Weight=0.5, Protein Weight Matrix=Gonnet Series, DNA Weight Matrix=IUB). After alignment of the sequences using the Clustal W program, it is possible to obtain a “percent identity” by viewing the “sequence distances” table in the same program. 
     It is well understood by one skilled in the art that many levels of sequence identity are useful in identifying polypeptides, from other species, wherein such polypeptides have the same or similar function or activity, or in describing the corresponding polynucleotides. Useful examples of percent identities include, but are not limited to: 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95%, or any integer percentage from 55% to 100% may be useful in describing the present invention, such as 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%. Suitable polynucleotide fragments not only have the above homologies but typically comprise a polynucleotide having at least 50 nucleotides, at least 100 nucleotides, at least 150 nucleotides, at least 200 nucleotides, or at least 250 nucleotides. Further, suitable polynucleotide fragments having the above homologies encode a polypeptide having at least 50 amino acids, at least 100 amino acids, at least 150 amino acids, at least 200 amino acids, or at least 250 amino acids. 
     The term “sequence analysis software” refers to any computer algorithm or software program that is useful for the analysis of nucleotide or amino acid sequences. “Sequence analysis software” may be commercially available or independently developed. Typical sequence analysis software will include, but is not limited to: 1.) the GCG suite of programs (Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison, Wis.); 2.) BLASTP, BLASTN, BLASTX (Altschul et al.,  J. Mol. Biol.,  215:403-410 (1990)); 3.) DNASTAR (DNASTAR, Inc. Madison, Wis.); 4.) SEQUENCHER (Gene Codes Corporation, Ann Arbor, Mich.); and 5.) the FASTA program incorporating the Smith-Waterman algorithm (W. R. Pearson,  Comput. Methods Genome Res ., [Proc. Int. Symp.] (1994),  Meeting Date  1992, 111-20. Editor(s): Suhai, Sandor. Plenum: New York, N.Y.). Within the context of this application it will be understood that where sequence analysis software is used for analysis, that the results of the analysis will be based on the “default values” of the program referenced, unless otherwise specified. As used herein “default values” will mean any set of values or parameters that originally load with the software when first initialized. 
     Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described by Sambrook, J., Fritsch, E. F. and Maniatis, T.,  Molecular Cloning: A Laboratory Manual , Second Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989) (hereinafter “Maniatis”); and by Silhavy, T. J., Bennan, M. L. and Enquist, L. W.,  Experiments with Gene Fusions , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1984); and by Ausubel, F. M. et al.,  Current Protocols in Molecular Biology , published by Greene Publishing Assoc. and Wiley-Interscience (1987). 
     The Functions of Fe—S Cluster-Requiring Proteins 
     The functions of proteins that contain Fe—S clusters are diverse. One of the more complete efforts to classify these functions is given in the following table which is adapted from Johnson, D. C., et al.,  Structure, function, and formation of biological iron - sulfur clusters . Annu. Rev. Biochem., 2005. 74: p. 247-281. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Functions of Biological [Fe—S] clusters a . 
               
            
           
           
               
               
               
            
               
                 Function 
                 Examples 
                 Cluster type 
               
               
                   
               
               
                 Electron transfer 
                 Ferredoxins; redox 
                 [2Fe—2S]; [3Fe—4S]; [4Fe—4S] 
               
               
                   
                 enzymes 
               
               
                 Coupled electron/proton 
                 Rieske protein 
                 [2Fe—2S] 
               
               
                 transfer 
                 Nitrogenase 
                 [8Fe—7S] 
               
               
                 Substrate binding and 
                 (de)Hydratases 
                 [4Fe—4S], [2Fe—2S] 
               
               
                 activation 
                 Radical SAM enzymes 
                 [4Fe—4S] 
               
               
                   
                 Acetyl-CoA synthase 
                 Ni—Ni—[4Fe—4S], [Ni—4Fe—5S] 
               
               
                   
                 Sulfite reductase 
                 [4Fe—4S]-siroheme 
               
               
                 Fe or cluster storage 
                 Ferredoxins 
                 [4Fe—4S] 
               
               
                   
                 Polyferredoxins 
                 [4Fe—4S] 
               
               
                 Structural 
                 Endonuclease III 
                 [4Fe—4S] 
               
               
                   
                 MutY 
                 [4Fe—4S] 
               
               
                 Regulation of gene 
                 SoxR 
                 [2Fe—2S] 
               
               
                 expression 
                 FNR 
                 [4Fe—4S]/[2Fe—2S] 
               
               
                   
                 IRP 
                 [4Fe—4S] 
               
               
                   
                 IscR 
                 [2Fe—2S] 
               
               
                 Regulation of enzyme 
                 Glutamine PRPP 
                 [4Fe—4S] 
               
               
                 activity 
                 amidotransferase 
               
               
                   
                 Ferrochelatase 
                 [2Fe—2S] 
               
               
                 Disulfide reduction 
                 Ferredoxin:thioredoxin 
                 [4Fe—4S] 
               
               
                   
                 reductase 
               
               
                   
                 Heterodisulfide 
                 [4Fe—4S] 
               
               
                   
                 reductase 
               
               
                 Sulfur donor 
                 Biotin synthase 
                 [2Fe—2S] 
               
               
                   
               
               
                   a Abbreviations used are SAM, S-adenosylmethionine; acetyl-CoA, acetyl coenzymeA; FNR, fumarate and nitrate reduction; IRP, iron-regulatory protein; IscR, iron-sulfur cluster assembly regulatory protein; PRPP, phosphoribosylpyrophosphate. 
               
            
           
         
       
     
     It is believed that an increase in the supply and the efficiency of loading Fe—S clusters into one or more of the members of the above classes will have commercial and/or medical benefits. Of the many possibilities that will be appreciated by the skilled artisan, three examples are given. 1) When an Fe—S cluster containing enzyme is used in a pathway to a fermentation product and needs to be expressed at high levels to maintain a high flux in the pathway to the product (e.g., dihydroxy-acid dehydratase in the pathway to isobutanol). 2) When an Fe—S cluster containing enzyme is used in a pathway to a fermentation product and the Fe—S cluster undergoes turnover during the catalysis (e.g., biotin synthase in the commercial fermentation of glucose to biotin). 3) In a diseased state such that the normal concentration of an Fe—S cluster containing protein important for good health is low (e.g., in cases of Friedreich&#39;s ataxia). 
     DHAD and DHAD Assays 
     DHAD is an Fe—S cluster requiring protein of the dehydratase (more properly hydro-lyase) class. A gene encoding a DHAD enzyme can be used to provide expression of DHAD activity in a recombinant host cell. DHAD catalyzes the conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate and of 2,3-dihydroxymethylvalerate to α-ketomethylvalerate and is classified as E.C. 4.2.1.9. Coding sequences for DHADs that are suitable for use in a recombinant host cell can be derived from bacterial, fungal, or plant sources. DHADs that may be used may have a [4Fe-4S] cluster or a [2Fe-2S]. Tables 4a, 4b, 5, and 6 list SEQ ID NOs for coding regions and proteins of representative DHADs that may be used in the present invention. Proteins with at least about 95% identity to certain listed sequences have been omitted for simplification, but it is understood that proteins, including those omitted for simplification, with at least about 95% sequence identity to any of the proteins listed in Tables 4a, 4b, 5, and 6 and having DHAD activity may be used as disclosed herein. Additional DHAD proteins and their encoding sequences may be identified by BLAST searching of public databases, as well known to one skilled in the art. Typically BLAST (described above) searching of publicly available databases with known DHAD sequences, such as those provided herein, is used to identify DHADs and their encoding sequences that may be expressed in the present cells. For example, DHAD proteins having amino acid sequence identities of at least about 80-85%, at least about 85-90%, at least about 90-95%, or at least about 98% sequence identity to any of the DHAD proteins of Table 3 may be expressed in the present cells. Identities are based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix. 
     
       
         
           
               
             
               
                 TABLE 4a 
               
             
            
               
                   
               
               
                 SEQ ID NOs of Representative Bacterial [2Fe—2S] DHAD 
               
               
                 Proteins and Encoding Sequences. 
               
            
           
           
               
               
               
            
               
                   
                 SEQ 
                   
               
               
                   
                 ID NO: 
                 SEQ 
               
               
                   
                 Nucleic 
                 ID NO: 
               
               
                 Organism of derivation 
                 acid 
                 Peptide 
               
               
                   
               
            
           
           
               
               
               
            
               
                   Mycobacterium  sp. MCS 
                 1 
                 2 
               
               
                   Mycobacterium gilvum  PYR-GCK 
                 3 
                 4 
               
               
                   Mycobacterium smegmatis  str. MC2 155 
                 5 
                 6 
               
               
                   Mycobacterium vanbaalenii  PYR-1 
                 7 
                 8 
               
               
                   Nocardia farcinica  IFM 10152 
                 9 
                 10 
               
               
                   Rhodococcus  sp. RHA1 
                 11 
                 12 
               
               
                   Mycobacterium ulcerans  Agy99 
                 13 
                 14 
               
               
                   Mycobacterium avium  subsp.  paratuberculosis  K-10 
                 15 
                 16 
               
               
                   Mycobacterium tuberculosis  H37Ra 
                 17 
                 18 
               
               
                   Mycobacterium leprae  TN * 
                 19 
                 20 
               
               
                   Kineococcus radiotolerans  SRS30216 
                 21 
                 22 
               
               
                   Janibacter  sp. HTCC2649 
                 23 
                 24 
               
               
                   Nocardioides  sp. JS614 
                 25 
                 26 
               
               
                   Renibacterium salmoninarum  ATCC 33209 
                 27 
                 28 
               
               
                   Arthrobacter aurescens  TC1 
                 29 
                 30 
               
               
                   Leifsonia xyli  subsp.  xyli  str. CTCB07 
                 31 
                 32 
               
               
                 marine actinobacterium PHSC20C1 
                 33 
                 34 
               
               
                   Clavibacter michiganensis  subsp.  michiganensis   
                 35 
                 36 
               
               
                 NCPPB 382 
               
               
                   Saccharopolyspora erythraea  NRRL 2338 
                 37 
                 38 
               
               
                   Acidothermus cellulolyticus  11B 
                 39 
                 40 
               
               
                   Corynebacterium efficiens  YS-314 
                 41 
                 42 
               
               
                   Brevibacterium linens  BL2 
                 43 
                 44 
               
               
                   Tropheryma whipplei  TW08/27 
                 45 
                 46 
               
               
                   Methylobacterium extorquens  PA1 
                 47 
                 48 
               
               
                   Methylobacterium nodulans  ORS 2060 
                 49 
                 50 
               
               
                   Rhodopseudomonas palustris  BisB5 
                 51 
                 52 
               
               
                   Rhodopseudomonas palustris  BisB18 
                 53 
                 54 
               
               
                   Bradyrhizobium  sp. ORS278 
                 55 
                 56 
               
               
                   Bradyrhizobium japonicum  USDA 110 
                 57 
                 58 
               
               
                   Fulvimarina pelagi  HTCC2506 
                 59 
                 60 
               
               
                   Aurantimonas  sp. SI85-9A1 
                 61 
                 62 
               
               
                   Hoeflea phototrophica  DFL-43 
                 63 
                 64 
               
               
                   Mesorhizobium loti  MAFF303099 
                 65 
                 66 
               
               
                   Mesorhizobium  sp. BNC1 
                 67 
                 68 
               
               
                   Parvibaculum lavamentivorans  DS-1 
                 69 
                 70 
               
               
                   Loktanella vestfoldensis  SKA53 
                 71 
                 72 
               
               
                   Roseobacter  sp. CCS2 
                 73 
                 74 
               
               
                   Dinoroseobacter shibae  DFL 12 
                 75 
                 76 
               
               
                   Roseovarius nubinhibens  ISM 
                 77 
                 78 
               
               
                   Sagittula stellata  E-37 
                 79 
                 80 
               
               
                   Roseobacter  sp. AzwK-3b 
                 81 
                 82 
               
               
                   Roseovarius  sp. TM1035 
                 83 
                 84 
               
               
                   Oceanicola batsensis  HTCC2597 
                 85 
                 86 
               
               
                   Oceanicola granulosus  HTCC2516 
                 87 
                 88 
               
               
                   Rhodobacterales bacterium  HTCC2150 
                 89 
                 90 
               
               
                   Paracoccus denitrificans  PD1222 
                 91 
                 92 
               
               
                   Oceanibulbus indolifex  HEL-45 
                 93 
                 94 
               
               
                   Sulfitobacter  sp. EE-36 
                 95 
                 96 
               
               
                   Roseobacter denitrificans  OCh 114 
                 97 
                 98 
               
               
                   Jannaschia  sp. CCS1 
                 99 
                 100 
               
               
                   Caulobacter  sp. K31 
                 101 
                 102 
               
               
                   Candidatus Pelagibacter  ubique HTCC1062 
                 103 
                 104 
               
               
                   Erythrobacter litoralis  HTCC2594 
                 105 
                 106 
               
               
                   Erythrobacter  sp. NAP1 
                 107 
                 108 
               
               
                   Comamonas testosterone  KF-1 
                 109 
                 110 
               
               
                   Sphingomonas wittichii  RW1 
                 111 
                 112 
               
               
                   Burkholderia xenovorans  LB400 
                 113 
                 114 
               
               
                   Burkholderia phytofirmans  PsJN 
                 115 
                 116 
               
               
                   Bordetella petrii  DSM 12804 
                 117 
                 118 
               
               
                   Bordetella bronchiseptica  RB50 
                 119 
                 120 
               
               
                   Bradyrhizobium  sp. ORS278 
                 121 
                 122 
               
               
                   Bradyrhizobium  sp. BTAi1 
                 123 
                 124 
               
               
                 
                   Bradhyrhizobium japonicum 
                 
                 125 
                 126 
               
               
                   Sphingomonas wittichii  RW1 
                 127 
                 128 
               
               
                   Rhodobacterales bacterium  HTCC2654 
                 129 
                 130 
               
               
                   Solibacter usitatus  Ellin6076 
                 131 
                 132 
               
               
                   Roseiflexus  sp. RS-1 
                 133 
                 134 
               
               
                   Rubrobacter xylanophilus  DSM 9941 
                 135 
                 136 
               
               
                   Salinispora tropica  CNB-440 
                 137 
                 138 
               
               
                   Acidobacteria bacterium  Ellin345 
                 139 
                 140 
               
               
                   Thermus thermophilus  HB27 
                 141 
                 142 
               
               
                   Maricaulis maris  MCS10 
                 143 
                 144 
               
               
                   Parvularcula bermudensis  HTCC2503 
                 145 
                 146 
               
               
                   Oceanicaulis alexandrii  HTCC2633 
                 147 
                 148 
               
               
                   Plesiocystis pacifica  SIR-1 
                 149 
                 150 
               
               
                   Bacillus  sp. NRRL B-14911 
                 151 
                 152 
               
               
                   Oceanobacillus iheyensis  HTE831 
                 153 
                 154 
               
               
                   Staphylococcus saprophyticus  subsp.  saprophyticus   
                 155 
                 156 
               
               
                 ATCC 15305 
               
               
                   Bacillus selenitireducens  MLS10 
                 157 
                 158 
               
               
                   Streptococcus pneumoniae  SP6-BS73 
                 159 
                 160 
               
               
                   Streptococcus sanguinis  SK36 
                 161 
                 162 
               
               
                   Streptococcus thermophilus  LMG 18311 
                 163 
                 164 
               
               
                   Streptococcus suis  89/1591 
                 165 
                 166 
               
               
                   Streptococcus mutans  UA159 
                 167 
                 168 
               
               
                   Leptospira borgpetersenii  serovar  Hardjo-bovis  L550 
                 169 
                 170 
               
               
                   Candidatus Vesicomyosocius okutanii  HA 
                 171 
                 172 
               
               
                   Candidatus Ruthia magnifica  str. Cm ( Calyptogena   
                 173 
                 174 
               
               
                   magnifica ) 
               
               
                   Methylococcus capsulatus  str. Bath 
                 175 
                 176 
               
               
                 uncultured marine bacterium EB80_02D08 
                 177 
                 178 
               
               
                 uncultured marine gamma proteobacterium 
                 179 
                 180 
               
               
                 EBAC31A08 
               
               
                 uncultured marine gamma proteobacterium 
                 181 
                 182 
               
               
                 EBAC20E09 
               
               
                 uncultured gamma proteobacterium eBACHOT4E07 
                 183 
                 184 
               
               
                   Alcanivorax borkumensis  SK2 
                 185 
                 186 
               
               
                   Chromohalobacter salexigens  DSM 3043 
                 187 
                 188 
               
               
                   Marinobacter algicola  DG893 
                 189 
                 190 
               
               
                   Marinobacter aquaeolei  VT8 
                 191 
                 192 
               
               
                   Marinobacter  sp. ELB17 
                 193 
                 194 
               
               
                   Pseudoalteromonas haloplanktis  TAC125 
                 195 
                 196 
               
               
                   Acinetobacter  sp. ADP1 
                 197 
                 198 
               
               
                   Opitutaceae bacterium  TAV2 
                 199 
                 200 
               
               
                   Flavobacterium  sp. MED217 
                 201 
                 202 
               
               
                   Cellulophaga  sp. MED134 
                 203 
                 204 
               
               
                   Kordia algicida  OT-1 
                 205 
                 206 
               
               
                   Flavobacteriales bacterium  ALC-1 
                 207 
                 208 
               
               
                   Psychroflexus torquis  ATCC 700755 
                 209 
                 210 
               
               
                   Flavobacteriales bacterium  HTCC2170 
                 211 
                 212 
               
               
                 unidentified eubacterium SCB49 
                 213 
                 214 
               
               
                   Gramella forsetii  KT0803 
                 215 
                 216 
               
               
                   Robiginitalea biformata  HTCC2501 
                 217 
                 218 
               
               
                   Tenacibaculum  sp. MED152 
                 219 
                 220 
               
               
                   Polaribacter irgensii  23-P 
                 221 
                 222 
               
               
                   Pedobacter  sp. BAL39 
                 223 
                 224 
               
               
                   Flavobacteria bacterium  BAL38 
                 225 
                 226 
               
               
                   Flavobacterium psychrophilum  JIP02/86 
                 227 
                 228 
               
               
                   Flavobacterium johnsoniae  UW101 
                 229 
                 230 
               
               
                   Lactococcus lactis  subsp.  cremoris  SK11 
                 231 
                 232 
               
               
                   Psychromonas ingrahamii  37 
                 233 
                 234 
               
               
                   Microscilla marina  ATCC 23134 
                 235 
                 236 
               
               
                   Cytophaga hutchinsonii  ATCC 33406 
                 237 
                 238 
               
               
                   Rhodopirellula baltica  SH 1 
                 239 
                 240 
               
               
                   Blastopirellula marina  DSM 3645 
                 241 
                 242 
               
               
                   Planctomyces maris  DSM 8797 
                 243 
                 244 
               
               
                   Algoriphagus  sp. PR1 
                 245 
                 246 
               
               
                   Candidatus Sulcia muelleri  str. Hc ( Homalodisca   
                 247 
                 248 
               
               
                   coagulata ) 
               
               
                   Candidatus Carsonella ruddii  PV 
                 249 
                 250 
               
               
                   Synechococcus  sp. RS9916 
                 251 
                 252 
               
               
                   Synechococcus  sp. WH 7803 
                 253 
                 254 
               
               
                   Synechococcus  sp. CC9311 
                 255 
                 256 
               
               
                   Synechococcus  sp. CC9605 
                 257 
                 258 
               
               
                   Synechococcus  sp. WH 8102 
                 259 
                 260 
               
               
                   Synechococcus  sp. BL107 
                 261 
                 262 
               
               
                   Synechococcus  sp. RCC307 
                 263 
                 264 
               
               
                   Synechococcus  sp. RS9917 
                 265 
                 266 
               
               
                   Synechococcus  sp. WH 5701 
                 267 
                 268 
               
               
                   Prochlorococcus marinus  str. MIT 9313 
                 269 
                 270 
               
               
                   Prochlorococcus marinus  str. NATL2A 
                 271 
                 272 
               
               
                   Prochlorococcus marinus  str. MIT 9215 
                 273 
                 274 
               
               
                   Prochlorococcus marinus  str. AS9601 
                 275 
                 276 
               
               
                   Prochlorococcus marinus  str. MIT 9515 
                 277 
                 278 
               
               
                   Prochlorococcus marinus  subsp.  pastoris  str. 
                 279 
                 280 
               
               
                 CCMP1986 
               
               
                   Prochlorococcus marinus  str. MIT 9211 
                 281 
                 282 
               
               
                   Prochlorococcus marinus  subsp.  marinus  str. 
                 283 
                 284 
               
               
                 CCMP1375 
               
               
                   Nodularia spumigena  CCY9414 
                 285 
                 286 
               
               
                   Nostoc punctiforme  PCC 73102 
                 287 
                 288 
               
               
                   Nostoc  sp. PCC 7120 
                 289 
                 290 
               
               
                   Trichodesmium erythraeum  IMS101 
                 291 
                 292 
               
               
                   Acaryochloris marina  MBIC11017 
                 293 
                 294 
               
               
                   Lyngbya  sp. PCC 8106 
                 295 
                 296 
               
               
                   Synechocystis  sp. PCC 6803 
                 297 
                 298 
               
               
                   Cyanothece  sp. CCY0110 
                 299 
                 300 
               
               
                   Thermosynechococcus elongatus  BP-1 
                 301 
                 302 
               
               
                   Synechococcus  sp. JA-2-3B′a(2-13) 
                 303 
                 304 
               
               
                   Gloeobacter violaceus  PCC 7421 
                 305 
                 306 
               
               
                   Nitrosomonas eutropha  C91 
                 307 
                 308 
               
               
                   Nitrosomonas europaea  ATCC 19718 
                 309 
                 310 
               
               
                   Nitrosospira multiformis  ATCC 25196 
                 311 
                 312 
               
               
                   Chloroflexus aggregans  DSM 9485 
                 313 
                 314 
               
               
                   Leptospirillum  sp. Group II UBA 
                 315 
                 316 
               
               
                   Leptospirillum  sp. Group II UBA 
                 317 
                 318 
               
               
                   Halorhodospira halophila  SL1 
                 319 
                 320 
               
               
                   Nitrococcus mobilis  Nb-231 
                 321 
                 322 
               
               
                   Alkalilimnicola ehrlichei  MLHE-1 
                 323 
                 324 
               
               
                   Deinococcus geothermalis  DSM 11300 
                 325 
                 326 
               
               
                   Polynucleobacter  sp. QLW-P1DMWA-1 
                 327 
                 328 
               
               
                   Polynucleobacter necessarius  STIR1 
                 329 
                 330 
               
               
                   Azoarcus  sp. EbN1 
                 331 
                 332 
               
               
                   Burkholderia phymatum  STM815 
                 333 
                 334 
               
               
                   Burkholderia xenovorans  LB400 
                 335 
                 336 
               
               
                   Burkholderia multivorans  ATCC 17616 
                 337 
                 338 
               
               
                   Burkholderia cenocepacia  PC184 
                 339 
                 340 
               
               
                   Burkholderia mallei  GB8 horse 4 
                 341 
                 342 
               
               
                   Ralstonia eutropha  JMP134 
                 343 
                 344 
               
               
                   Ralstonia metallidurans  CH34 
                 345 
                 346 
               
               
                   Ralstonia solanacearum  UW551 
                 347 
                 348 
               
               
                   Ralstonia pickettii  12J 
                 349 
                 350 
               
               
                   Limnobacter  sp. MED105 
                 351 
                 352 
               
               
                 
                   Herminiimonas arsenicoxydans 
                 
                 353 
                 354 
               
               
                 
                   Bordetella parapertussis 
                 
                 355 
                 356 
               
               
                   Bordetella petrii  DSM 12804 
                 357 
                 358 
               
               
                   Polaromonas  sp. JS666 
                 359 
                 360 
               
               
                   Polaromonas naphthalenivorans  CJ2 
                 361 
                 362 
               
               
                   Rhodoferax ferrireducens  T118 
                 363 
                 364 
               
               
                   Verminephrobacter eiseniae  EF01-2 
                 365 
                 366 
               
               
                   Acidovorax  sp. JS42 
                 367 
                 368 
               
               
                   Delftia acidovorans  SPH-1 
                 369 
                 370 
               
               
                   Methylibium petroleiphilum  PM1 
                 371 
                 372 
               
               
                 gamma proteobacterium KT 71 
                 373 
                 374 
               
               
                 
                   Tremblaya princes 
                 
                 375 
                 376 
               
               
                   Blastopirellula marina  DSM 3645 
                 377 
                 378 
               
               
                   Planctomyces maris  DSM 8797 
                 379 
                 380 
               
               
                   Microcystis aeruginosa  PCC 7806 
                 381 
                 382 
               
               
                   Salinibacter ruber  DSM 13855 
                 383 
                 384 
               
               
                 
                   Methylobacterium chloromethanicum 
                 
                 385 
                 386 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4B 
               
             
            
               
                   
               
               
                 Additional representative bacterial [2Fe—2S] DHAD proteins and 
               
               
                 encoding sequences. 
               
            
           
           
               
               
               
            
               
                   
                 Nucleic acid 
                 Amino acid 
               
               
                 Organism of derivation 
                 SEQ ID NO: 
                 SEQ ID NO: 
               
               
                   
               
               
                   Burkholderia ambifaria  AMMD 
                 387 
                 388 
               
               
                   Bradyrhizobium  sp. BTAi1 
                 389 
                 390 
               
               
                   Delftia acidovorans  SPH-1 
                 391 
                 392 
               
               
                   Microcystis aeruginosa  NIES-843 
                 393 
                 394 
               
               
                 uncultured marine microorganism 
                 395 
                 396 
               
               
                 HF4000_APKG8C21 
               
               
                   Burkholderia ubonensis  Bu 
                 397 
                 398 
               
               
                   Gemmata obscuriglobus  UQM 2246 
                 399 
                 400 
               
               
                 
                   Mycobacterium abscessus 
                 
                 401 
                 402 
               
               
                   Synechococcus  sp. PCC 7002 
                 403 
                 404 
               
               
                   Burkholderia graminis  C4D1M 
                 405 
                 406 
               
               
                   Methylobacterium radiotolerans  JCM 2831 
                 407 
                 408 
               
               
                   Leptothrix cholodnii  SP-6 
                 409 
                 410 
               
               
                   Verrucomicrobium spinosum  DSM 4136 
                 411 
                 412 
               
               
                   Cyanothece  sp. ATCC 51142 
                 413 
                 414 
               
               
                   Opitutus terrae  PB90-1 
                 415 
                 416 
               
               
                   Leptospira biflexa  serovar Patoc strain 
                 417 
                 418 
               
               
                 ‘Patoc 1 (Paris)’ 
               
               
                   Methylacidiphilum infernorum  V4 
                 419 
                 420 
               
               
                 
                   Cupriavidus taiwanensis 
                 
                 421 
                 422 
               
               
                   Chthoniobacter flavus  Ellin428 
                 423 
                 424 
               
               
                   Cyanothece  sp. PCC 7822 
                 425 
                 426 
               
               
                   Phenylobacterium zucineum  HLK1 
                 427 
                 428 
               
               
                   Leptospirillum  sp. Group II ‘5-way CG’ 
                 429 
                 430 
               
               
                   Arthrospira maxima  CS-328 
                 431 
                 432 
               
               
                   Oligotropha carboxidovorans  OM5 
                 433 
                 434 
               
               
                   Rhodospirillum centenum  SW 
                 435 
                 436 
               
               
                   Cyanothece  sp. PCC 8801 
                 437 
                 438 
               
               
                   Thermus aquaticus  Y51MC23 
                 439 
                 440 
               
               
                   Cyanothece  sp. PCC 7424 
                 441 
                 442 
               
               
                   Acidithiobacillus ferrooxidans  ATCC 23270 
                 443 
                 444 
               
               
                   Cyanothece  sp. PCC 7425 
                 445 
                 446 
               
               
                   Arthrobacter chlorophenolicus  A6 
                 447 
                 448 
               
               
                   Burkholderia multivorans  CGD2M 
                 449 
                 450 
               
               
                   Thermomicrobium roseum  DSM 5159 
                 451 
                 452 
               
               
                 bacterium Ellin514 
                 453 
                 454 
               
               
                   Desulfobacterium autotrophicum  HRM2 
                 455 
                 456 
               
               
                   Thioalkalivibrio  sp. K90mix 
                 457 
                 458 
               
               
                   Flavobacteria bacterium  MS024-3C 
                 459 
                 460 
               
               
                   Flavobacteria bacterium  MS024-2A 
                 461 
                 462 
               
               
                 ‘ Nostoc azollae ’ 0708 
                 463 
                 464 
               
               
                   Acidobacterium capsulatum  ATCC 51196 
                 465 
                 466 
               
               
                   Gemmatimonas aurantiaca  T-27 
                 467 
                 468 
               
               
                   Gemmatimonas aurantiaca  T-27 
                 469 
                 470 
               
               
                   Rhodococcus erythropolis  PR4 
                 471 
                 472 
               
               
                   Deinococcus deserti  VCD115 
                 473 
                 474 
               
               
                   Rhodococcus opacus  B4 
                 475 
                 476 
               
               
                   Chryseobacterium gleum  ATCC 35910 
                 477 
                 478 
               
               
                   Thermobaculum terrenum  ATCC BAA-798 
                 479 
                 480 
               
               
                   Kribbella flavida  DSM 17836 
                 481 
                 482 
               
               
                   Gordonia bronchialis  DSM 43247 
                 483 
                 484 
               
               
                   Geodermatophilus obscurus  DSM 43160 
                 485 
                 486 
               
               
                   Xylanimonas cellulosilytica  DSM 15894 
                 487 
                 488 
               
               
                   Sphingobacterium spiritivorum  ATCC 33300 
                 489 
                 490 
               
               
                   Meiothermus silvanus  DSM 9946 
                 491 
                 492 
               
               
                   Meiothermus ruber  DSM 1279 
                 493 
                 494 
               
               
                   Nakamurella multipartita  DSM 44233 
                 495 
                 496 
               
               
                   Cellulomonas flavigena  DSM 20109 
                 497 
                 498 
               
               
                   Rhodothermus marinus  DSM 4252 
                 499 
                 500 
               
               
                   Planctomyces limnophilus  DSM 3776 
                 501 
                 502 
               
               
                   Beutenbergia cavernae  DSM 12333 
                 503 
                 504 
               
               
                   Spirosoma linguale  DSM 74 
                 505 
                 506 
               
               
                   Sphaerobacter thermophilus  DSM 20745 
                 507 
                 508 
               
               
                 
                   Lactococcus lactis 
                 
                 509 
                 510 
               
               
                   Thermus thermophilus  HB8 
                 511 
                 512 
               
               
                   Anabaena variabilis  ATCC 29413 
                 513 
                 514 
               
               
                   Roseovarius  sp. 217 
                 515 
                 516 
               
               
                 uncultured  Prochlorococcus marinus   
                 517 
                 518 
               
               
                 clone HF10-88D1 
               
               
                   Burkholderia xenovorans  LB400 
                 519 
                 520 
               
               
                   Saccharomonospora viridis  DSM 43017 
                 521 
                 522 
               
               
                   Pedobacter heparinus  DSM 2366 
                 523 
                 524 
               
               
                   Microcoleus chthonoplastes  PCC 7420 
                 525 
                 526 
               
               
                   Acidimicrobium ferrooxidans  DSM 10331 
                 527 
                 528 
               
               
                   Rhodobacterales bacterium  HTCC2083 
                 529 
                 530 
               
               
                   Candidatus Pelagibacter  sp. HTCC7211 
                 531 
                 532 
               
               
                   Chitinophaga pinensis  DSM 2588 
                 533 
                 534 
               
               
                   Alcanivorax  sp. DG881 
                 535 
                 536 
               
               
                   Micrococcus luteus  NCTC 2665 
                 537 
                 538 
               
               
                   Verrucomicrobiae bacterium  DG1235 
                 539 
                 540 
               
               
                   Synechococcus  sp. PCC 7335 
                 541 
                 542 
               
               
                   Brevundimonas  sp. BAL3 
                 543 
                 544 
               
               
                   Dyadobacter fermentans  DSM 18053 
                 545 
                 546 
               
               
                 gamma proteobacterium NOR5-3 
                 547 
                 548 
               
               
                 gamma proteobacterium NOR51-B 
                 549 
                 550 
               
               
                   Cyanobium  sp. PCC 7001 
                 551 
                 552 
               
               
                   Jonesia denitrificans  DSM 20603 
                 553 
                 554 
               
               
                   Brachybacterium faecium  DSM 4810 
                 555 
                 556 
               
               
                   Paenibacillus  sp. JDR-2 
                 557 
                 558 
               
               
                   Octadecabacter antarcticus  307 
                 559 
                 560 
               
               
                   Variovorax paradoxus  S110 
                 561 
                 562 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 SEQ ID NOs of Representative Fungal and Plant [2Fe—2S] 
               
               
                 DHAD Proteins and Encoding Sequences. 
               
            
           
           
               
               
               
            
               
                   
                 SEQ ID NO: 
                 SEQ ID NO: 
               
               
                 Description 
                 Nucleic acid 
                 Peptide 
               
               
                   
               
               
                   Schizosaccharomyces pombe  ILV3 
                 563 
                 564 
               
               
                   Saccharomyces cerevisiae  ILV3 
                 565 
                 566 
               
               
                   Kluyveromyces lactis  ILV3 
                 567 
                 568 
               
               
                   Candida albicans  SC5314 ILV3 
                 569 
                 570 
               
               
                   Pichia stipitis  CBS 6054 ILV3 
                 571 
                 572 
               
               
                   Yarrowia lipolytica  ILV3 
                 573 
                 574 
               
               
                   Candida galbrata  CBS 138 ILV3 
                 575 
                 576 
               
               
                 
                   Chlamydomonas reinhardtii 
                 
                 577 
                 578 
               
               
                   Ostreococcus lucimarinus  CCE9901 
                 579 
                 580 
               
               
                 
                   Vitis vinifera 
                 
                 581 
                 582 
               
               
                 (Unnamed protein product: CAO71581.1) 
               
               
                 
                   Vitis vinifera 
                 
                 583 
                 584 
               
               
                 (Hypothetical protein: CAN67446.1) 
               
               
                 
                   Arabidopsis thaliana 
                 
                 585 
                 586 
               
               
                   Oryza sativa  ( indica  cultivar-group) 
                 587 
                 588 
               
               
                   Physcomitrella patens  subsp.  Patens   
                 589 
                 590 
               
               
                   Chaetomium globosum  CBS 148.51 
                 591 
                 592 
               
               
                   Neurospora crassa  OR74A 
                 593 
                 594 
               
               
                 
                   Magnaporthe grisea 70-15 
                 
                 595 
                 596 
               
               
                   Gibberella zeae  PH-1 
                 597 
                 598 
               
               
                 
                   Aspergillus niger 
                 
                 599 
                 600 
               
               
                   Neosartorya fischeri  NRRL 181 
                 601 
                 602 
               
               
                 (XP_001266525.1) 
               
               
                   Neosartorya fischeri  NRRL 181 
                 603 
                 604 
               
               
                 (XP_001262996.1) 
               
               
                 
                   Aspergillus niger 
                 
                 605 
                 606 
               
               
                 (hypothetical protein An03g04520) 
               
               
                 
                   Aspergillus niger 
                 
                 607 
                 608 
               
               
                 (Hypothetical protein An14g03280) 
               
               
                   Aspergillus terreus  NIH2624 
                 609 
                 610 
               
               
                   Aspergillus clavatus  NRRL 1 
                 611 
                 612 
               
               
                   Aspergillus nidulans  FGSC A4 
                 613 
                 614 
               
               
                 
                   Aspergillus oryzae 
                 
                 615 
                 616 
               
               
                   Ajellomyces capsulatus  NAm1 
                 617 
                 618 
               
               
                   Coccidioides immitis  RS 
                 619 
                 620 
               
               
                   Botryotinia fuckeliana  B05.10 
                 621 
                 622 
               
               
                   Phaeosphaeria nodorum  SN15 
                 623 
                 624 
               
               
                   Pichia guilliermondii  ATCC 6260 
                 625 
                 626 
               
               
                   Debaryomyces hansenii  CBS767 
                 627 
                 628 
               
               
                   Lodderomyces elongisporus  NRRL YB-4239 
                 629 
                 630 
               
               
                   Vanderwaltozyma polyspora  DSM 70294 
                 631 
                 632 
               
               
                   Ashbya gossypii  ATCC 10895 
                 633 
                 634 
               
               
                   Laccaria bicolor  S238N-H82 
                 635 
                 636 
               
               
                   Coprinopsis cinerea  okayama 7#130 
                 637 
                 638 
               
               
                   Cryptococcus neoformans  var. 
                 639 
                 640 
               
               
                   neoformans  JEC21 
               
               
                 
                   Ustilago maydis 521 
                 
                 641 
                 642 
               
               
                   Malassezia globosa  CBS 7966 
                 643 
                 644 
               
               
                   Aspergillus clavatus  NRRL 1 
                 645 
                 646 
               
               
                   Neosartorya fischeri  NRRL 181 
                 647 
                 648 
               
               
                 (Putative) 
               
               
                 
                   Aspergillus oryzae 
                 
                 649 
                 650 
               
               
                   Aspergillus niger  (hypothetical 
                 651 
                 652 
               
               
                 protein An18g04160) 
               
               
                   Aspergillus terreus  NIH2624 
                 653 
                 654 
               
               
                   Coccidioides immitis  RS (hypothetical 
                 655 
                 656 
               
               
                 protein CIMG_04591) 
               
               
                 
                   Paracoccidioides brasiliensis 
                 
                 657 
                 658 
               
               
                   Phaeosphaeria nodorum  SN15 
                 659 
                 660 
               
               
                   Gibberella zeae  PH-1 
                 661 
                 662 
               
               
                   Neurospora crassa  OR74A 
                 663 
                 664 
               
               
                   Coprinopsis cinerea  okayama 7#130 
                 665 
                 666 
               
               
                   Laccaria bicolor  S238N-H82 
                 667 
                 668 
               
               
                   Ustilago maydis  521 
                 669 
                 670 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 SEQ ID NOs of Representative [4Fe—4S] 
               
               
                 DHAD Proteins and Encoding Sequences. 
               
            
           
           
               
               
               
            
               
                   
                 SEQ ID NO: 
                 SEQ ID NO: 
               
               
                 Organism 
                 Nucleic acid 
                 Peptide 
               
               
                   
               
               
                   Escherichia coli  str. K-12 substr. MG1655 
                 671 
                 672 
               
               
                   Bacillus subtilis  subsp.  subtilis  str. 168 
                 673 
                 674 
               
               
                   Agrobacterium tumefaciens  str. C58 
                 675 
                 676 
               
               
                   Burkholderia cenocepacia  MC0-3 
                 677 
                 678 
               
               
                   Psychrobacter cryohalolentis  K5 
                 679 
                 680 
               
               
                   Psychromonas  sp. CNPT3 
                 681 
                 682 
               
               
                   Deinococcus radiodurans  R1 
                 683 
                 684 
               
               
                   Wolinella succinogenes  DSM 1740 
                 685 
                 686 
               
               
                   Zymomonas mobilis  subsp.  mobilis  ZM4 
                 687 
                 688 
               
               
                   Clostridium acetobutylicum  ATCC 824 
                 689 
                 690 
               
               
                   Clostridium beijerinckii  NCIMB 8052 
                 691 
                 692 
               
               
                   Pseudomonas fluorescens  Pf-5 
                 693 
                 694 
               
               
                   Methanococcus maripaludis  C7 
                 695 
                 696 
               
               
                   Methanococcus aeolicus  Nankai-3 
                 697 
                 698 
               
               
                   Vibrio fischeri  ATCC 700601 (ES114) 
                 699 
                 700 
               
               
                   Shewanella oneidensis  MR-1 ATCC 700550 
                 701 
                 702 
               
               
                   
               
            
           
         
       
     
     Additional [2Fe-2S] DHADs may be identified using the analysis described in U.S. patent application Ser. No. 12/569,636, filed Sep. 29, 2009, which is herein incorporated by reference. The analysis is as follows: A Profile Hidden Markov Model (HMM) was prepared based on amino acid sequences of eight functionally verified DHADs. The application of Profile HMM has been described. See, e.g., Krogh et al.,  J. Mol. Biol.  235:1501-1531 (1994) and Durbin et al., “Markov chains and hidden Markov models,” in Biological Sequence Analysis: Probabilistic Models of Proteins and Nucleic Acids, Cambridge University Press (1998). A Profile HMM is a statistical model built of multiple sequence alignments that can be used to determine whether or not a test sequence belongs to a particular family of sequences. See id. A Profile HMM can be built by first generating an alignment of functionally verified sequences using conventional sequence alignment tools. Next, the sequence alignment is used to build the Profile HMM using publicly available software programs (e.g., HMMER) that use a position-specific scoring system to capture information about the degree of conservation at various amino acid positions in the multiple alignment of the input sequences. More specifically, the scores of amino acid residues in a “match” state (i.e., match state emission scores), or in an “insert” state (i.e., insert state emission scores) are captured which are proportional to the expression: Log_2 (p_x)/(null_x). See id. In this expression, the term “p_x” is the probability of an amino acid residue, at a particular position in the alignment, according to the Profile HMM, and the term “null_x” is the probability according to the Null model. See id. The Null model is a simple one state probabilistic model with a pre-calculated set of emission probabilities for each of the amino acids derived from the distribution of amino acids. See id. “State” transition scores are also calculated as log odds parameters and are proportional to Log_2 (t_x). See id. In this expression, the term “t_x” is the probability of transiting to an emitter or non-emitter state. See id. Further details regarding the particular statistical analyses to generate a Profile HMM are available in Krogh et al.,  J. Mol. Biol.  235:1501-1531 (1994) and Durbin et al., “Markov chains and hidden Markov models,” in Biological Sequence Analysis: Probabilistic Models of Proteins and Nucleic Acids, Cambridge University Press (1998), and U.S. patent application Ser. No. 12/569,636. 
     A Profile Hidden Markov Model (HMM) was prepared based on amino acid sequences of eight functionally verified DHADs are from  Nitrosomonas europaea  (DNA SEQ ID NO:309; protein SEQ ID NO:310),  Synechocystis  sp. PCC6803 (DNA SEQ ID:297; protein SEQ ID NO:298),  Streptococcus mutans  (DNA SEQ ID NO:167; protein SEQ ID NO:168),  Streptococcus thermophilus  (DNA SEQ ID NO:163; SEQ ID No:164),  Ralstonia metallidurans  (DNA SEQ ID NO:345; protein SEQ ID NO:346),  Ralstonia eutropha  (DNA SEQ ID NO:343; protein SEQ ID NO:344), and  Lactococcus lactis  (DNA SEQ ID NO:231; protein SEQ ID NO:232). In addition the DHAD from  Flavobacterium johnsoniae  (DNA SEQ ID NO:229; protein SEQ ID NO:230) was found to have dihydroxy-acid dehydratase activity when expressed in  E. coli  and was used in making the Profile. The Profile HMM is prepared using the HMMER software package (The theory behind profile HMMs is described in R. Durbin, S. Eddy, A. Krogh, and G. Mitchison, Biological sequence analysis: probabilistic models of proteins and nucleic acids, Cambridge University Press, 1998; Krogh et al., 1994 ; J. Mol. Biol.  235:1501-1531), following the user guide which is available from HMMER (Janelia Farm Research Campus, Ashburn, Va.). The output of the HMMER software program is a Profile Hidden Markov Model (HMM) that characterizes the input sequences. The Profile HMM prepared for the eight DHAD proteins is given in U.S. application Ser. No. 12/569,636, filed Sep. 29, 2009 and in Table 12. 
     The first line in Table 12 for each position reports the probability for each amino acid to be in that “state” (match state emission scores). The second line reports the insert state emission scores, and the third line reports the state transition scores. The highest probability is highlighted for each position. These scores can be converted into “E values” (expectation values), which are the number of hits or matches to the Profile HMM one would expect to obtain just by chance. A protein having an E value of &lt;10 −5  match to the Profile HMM, indicates that the protein shares significant sequence similarity with the seed proteins used to construct the Profile HMM and that the protein belongs to the family represented by the profile HMM. 
     Any protein that matches the Profile HMM with an E value of &lt;10 −5  is a DHAD related protein, which includes [4Fe-4S] DHADs, [2Fe-2S] DHADs, arabonate dehydratases, and phosphogluconate dehydratases. In embodiments, sequences matching the Profile HMM are then analyzed for the presence of the three conserved cysteines, corresponding to positions 56, 129, and 201 in the  Streptococcus mutans  DHAD. The presence of all three conserved cysteines is characteristic of proteins having a [2Fe-2S] cluster. Proteins having the three conserved cysteines include arabonate dehydratases and [2Fe-2S] DHADs. The [2Fe-2S] DHADs may be distinguished from the arabonate dehydratases by analyzing for signature conserved amino acids found to be present in the [2Fe-2S] DHADs or in the arabonate dehydratases at positions corresponding to the following positions in the  Streptococcus mutans  DHAD amino acid sequence. These signature amino acids are in [2Fe-2S] DHADs or in arabonate dehydratases, respectively, at the following positions (with greater than 90% occurrence): 88 asparagine vs. glutamic acid; 113 not conserved vs. glutamic acid; 142 arginine or asparagine vs. not conserved; 165 not conserved vs. glycine; 208 asparagine vs. not conserved; 454 leucine vs. not conserved; 477 phenylalanine or tyrosine vs. not conserved; and 487 glycine vs. not conserved. 
     Additionally, the sequences of DHAD coding regions provided herein may be used to identify other homologs in nature. Such methods are well-known in the art, and various methods that may be used to isolate genes encoding homologous proteins are described in U.S. application Ser. No. 12/569,636, filed Sep. 29, 2009, which such methods are incorporated by reference herein. 
     The presence of DHAD activity in a cell engineered to express a heterologous DHAD can be confirmed using methods known in the art. As one example, and as demonstrated in the Examples herein, crude extracts from cells engineered to express a bacterial DHAD may be used in a DHAD assay as described by Flint and Emptage ( J. Biol. Chem . (1988) 263(8): 3558-64) using dinitrophenylhydrazine. In another example, DHAD activity may be assayed by expressing a heterologous DHAD identifiable by the methods disclosed herein in a yeast strain that lacks endogenous DHAD activity. If DHAD activity is present, the yeast strain will grow in the absence of branched-chain amino acids. DHAD activity may also be confirmed by more indirect methods, such as by assaying for a downstream product in a pathway requiring DHAD activity. Any product that has α-ketoisovalerate or α-ketomethylvalerate as a pathway intermediate may be measured in an assay for DHAD activity. A list of such products includes, but is not limited to, valine, isoleucine, leucine, pantothenic acid, 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol. 
     Over-Expression of DHAD Activity 
     Applicants have found that expression of a heterologous DHAD can provide DHAD activity when expressed in a host cell. Expression of a DHAD which may be identified as described herein can provide DHAD activity for a biosynthetic pathway that includes conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. In addition, the  S. mutans [ 2Fe-2S] DHAD was shown in related U.S. application Ser. No. 12/569,636, filed Sep. 29, 2009, incorporated by reference herein, to have higher stability in air as compared to the sensitivity in air of the  E. coli [ 4Fe-4S] DHAD, which is desirable for obtaining better activity in a heterologous host cell. 
     Furthermore, as described herein, it has been found that expressing a heterologous DHAD protein at higher levels can provide increased DHAD activity when expressed in a host cell. High expression of a recombinant polynucleotide can be accomplished in at least two ways: 1) by increasing the copy number of a plasmid comprising the recombinant polynucleotide; or 2) by integrating multiple copies of the gene of interest into the host cell&#39;s chromosome. As exemplified herein, expression of multiple copies of the heterologous DHAD, provides an increase in specific activity of heterologous DHAD 
     Recombinant polynucleotides are typically cloned for expression using the coding sequence as part of a chimeric gene used for transformation, which includes a promoter operably linked to the coding sequence as well as a ribosome binding site and a termination control region. The coding region may be from the host cell for transformation and combined with regulatory sequences that are not native to the natural gene encoding DHAD. Alternatively, the coding region may be from another host cell. 
     Vectors useful for the transformation of a variety of host cells are common and described in the literature. Typically the vector contains a selectable marker and sequences allowing autonomous replication or chromosomal integration in the desired host. In addition, suitable vectors may comprise a promoter region which harbors transcriptional initiation controls and a transcriptional termination control region, between which a coding region DNA fragment may be inserted, to provide expression of the inserted coding region. Both control regions may be derived from genes homologous to the transformed host cell, although it is to be understood that such control regions may also be derived from genes that are not native to the specific species chosen as a production host. 
     Yeast cells that can be hosts for expression or over-expression of a heterologous bacterial DHAD are any yeast cells that are amenable to genetic manipulation and include, but are not limited to,  Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia , and  Pichia . Suitable strains include, but are not limited to,  Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces thermotolerans, Candida glabrata, Candida albicans, Pichia stipitis  and  Yarrowia lipolytica . In one embodiment, the host is  Saccharomyces cerevisiae.    
     Expression is achieved by transforming a host cell with a gene comprising a sequence encoding DHAD, for example, a DHAD listed in Tables 4a, 4b, 5 or 6, or identified using the screening methods in related U.S. application Ser. No. 12/569,636, filed Sep. 29, 2009, incorporated by reference herein. The coding region for the DHAD to be expressed may be codon optimized for the target host cell, as well known to one skilled in the art. Methods for gene expression in yeast are known in the art (see, e.g., Methods in Enzymology, Volume 194, Guide to Yeast Genetics and Molecular and Cell Biology (Part A, 2004, Christine Guthrie and Gerald R. Fink (Eds.), Elsevier Academic Press, San Diego, Calif.). Expression of genes in yeast typically requires a promoter, operably linked to a coding region of interest, and a transcriptional terminator. A number of yeast promoters can be used in constructing expression cassettes for genes in yeast, including, but not limited to, promoters derived from the following genes: CYC1, HIS3, GAL1, GAL10, ADH1, PGK, PHO5, GAPDH, ADC1, TRP1, URA3, LEU2, ENO, TPI, CUP1, FBA, GPD, GPM, and AOX1. Suitable transcriptional terminators include, but are not limited to, FBAt, GPDt, GPMt, ERG10t, GAL1t, CYC1, and ADH1. 
     Suitable promoters, transcriptional terminators, and DHAD coding regions may be cloned into  E. coli -yeast shuttle vectors, and transformed into yeast cells. These vectors allow strain propagation in both  E. coli  and yeast strains. In one embodiment, the vector used contains a selectable marker and sequences allowing autonomous replication or chromosomal integration in the desired host. Examples of plasmids used in yeast are shuttle vectors pRS423, pRS424, pRS425, and pRS426 (American Type Culture Collection, Manassas, Va.), which contain an  E. coli  replication origin (e.g., pMB1), a yeast 2-micron origin of replication, and a marker for nutritional selection. The selection markers for these four vectors are His3 (vector pRS423), Trp1 (vector pRS424), Leu2 (vector pRS425) and Ura3 (vector pRS426). Construction of expression vectors with a chimeric gene encoding the described DHADs can be performed by either standard molecular cloning techniques in  E. coli  or by the gap repair recombination method in yeast. 
     The gap repair cloning approach takes advantage of the highly efficient homologous recombination in yeast. For example, a yeast vector DNA is digested (e.g., in its multiple cloning site) to create a “gap” in its sequence. A number of insert DNAs of interest are generated that contain a ≥21 bp sequence at both the 5′ and the 3′ ends that sequentially overlap with each other, and with the 5′ and 3′ terminus of the vector DNA. For example, to construct a yeast expression vector for “Gene X,” a yeast promoter and a yeast terminator are selected for the expression cassette. The promoter and terminator are amplified from the yeast genomic DNA, and Gene X is either PCR amplified from its source organism or obtained from a cloning vector comprising Gene X sequence. There is at least a 21 bp overlapping sequence between the 5′ end of the linearized vector and the promoter sequence, between the promoter and Gene X, between Gene X and the terminator sequence, and between the terminator and the 3′ end of the linearized vector. The “gapped” vector and the insert DNAs are then co-transformed into a yeast strain and plated on the medium containing the appropriate compound mixtures that allow complementation of the nutritional selection markers on the plasmids. The presence of correct insert combinations can be confirmed by PCR mapping using plasmid DNA prepared from the selected cells. The plasmid DNA isolated from yeast (usually low in concentration) can then be transformed into an  E. coli  strain, e.g. TOP10, followed by mini preps and restriction mapping to further verify the plasmid construct. Finally, the construct can be verified by sequence analysis. 
     Like the gap repair technique, integration into the yeast genome also takes advantage of the homologous recombination system in yeast. For example, a cassette containing a coding region plus control elements (promoter and terminator) and auxotrophic marker is PCR-amplified with a high-fidelity DNA polymerase using primers that hybridize to the cassette and contain 40-70 base pairs of sequence homology to the regions 5′ and 3′ of the genomic area where insertion is desired. The PCR product is then transformed into yeast and plated on medium containing the appropriate compound mixtures that allow selection for the integrated auxotrophic marker. For example, to integrate “Gene X” into chromosomal location “Y”, the promoter-coding regionX-terminator construct is PCR amplified from a plasmid DNA construct and joined to an autotrophic marker (such as URA3) by either SOE PCR or by common restriction digests and cloning. The full cassette, containing the promoter-coding regionX-terminator-URA3 region, is PCR amplified with primer sequences that contain 40-70 bp of homology to the regions 5′ and 3′ of location “Y” on the yeast chromosome. The PCR product is transformed into yeast and selected on growth media lacking uracil. Transformants can be verified either by colony PCR or by direct sequencing of chromosomal DNA. 
     In addition to the above materials and methods that may be used to express a heterologous DHAD, these same, or similar, materials and methods may be used to over-express a heterologous DHAD using modifications known to one of skill in the art. For example, when using a plasmid-based system to over-express the recombinant polynucleotide, a high-copy number vector, or a vector with a copy number that can be regulated, may be constructed. Such a regulatable or inducible system is described herein in Example 1; however, other systems are known to one of skill in the art and may be used to construct other high-copy number or copy number regulatable vectors. Alternatively, when using an integration-based system to over-express the recombinant polypeptide, an integration vector is required for targeting at multiple integration sites. A multiple integration-based system is described herein in Example 2; however, other multiple integration-based systems are known to one of skill in the art and may be used to target multiple integrations of a recombinant polypeptide, for example integration into rDNA regions. 
     Expression of the heterologous DHAD in the recombinant host cell can be quantified, e.g., by a percent of total cell protein. Such over-expression can be quantified in an amount selected from the group consisting of: (a) greater than about 0.001% of total cell protein; (b) greater than about 0.01% of total cell protein; (c) greater than about 0.1% of total cell protein; (d) greater than about 0.5% of total cell protein; (e) greater than about 1.0% of total cell protein; (f) greater than about 2.0% of total cell protein; (g) greater than about 5% of total cell protein; (h) greater than about 10% of total cell protein; and (i) greater than about 20% of total cell protein. 
     The specific activity of the heterologous DHAD produced in a recombinant host cell can be quantified, e.g., as U/mg. The heterologous DHAD specific activity can be selected from the group consisting of: (a) greater than about 0.25 U/mg; (b) greater than about 0.3 U/mg; (c) greater than about 0.5 U/mg; (d) greater than about 1.0 U/mg; (e) greater than about 1.5 U/mg; (f) greater than about 2.0 U/mg; (g) greater than about 3.0 U/mg; (h) greater than about 4.0 U/mg; (i) greater than about 5.0 U/mg; (j) greater than about 6.0 U/mg; (k) greater than about 7.0 U/mg; (l) greater than about 8.0 U/mg; (m) greater than about 9.0 U/mg; (n) greater than about 10.0 U/mg; (o) greater than about 20.0 U/mg; and (p) greater than about 50.0 U/mg. 
     The heterologous DHAD specific activity can also be quantified, e.g., as a percent comparison to an endogenous DHAD specific activity or to some other control DHAD specific activity. An example of a “control” DHAD specific activity is that from a heterologous DHAD expressed in a recombinant host cell using a low copy number plasmid or a plasmid that is not otherwise inducible or regulatable. Such a control establishes a baseline from which to compare the specific activity of the same heterologous DHAD expressed in a recombinant host cell using a high copy number plasmid or a plasmid with copy number that can be regulated, or co-expressed with polynucleotides encoding polypeptides affecting Fe—S cluster biosynthesis or Fe uptake and utilization, as described below. Thus, the increase in specific activity of the heterologous DHAD when compared to the control DHAD specific activity can be in an amount selected from the group consisting of: greater than an about 10% increase; greater than an about 20% increase; greater than an about 30% increase; greater than an about 40% increase; greater than an about 50% increase; greater than an about 60% increase; greater than an about 70% increase; greater than an about 80% increase; greater than an about 90% increase; greater than an about 95% increase; greater than an about 98% increase; and greater than an about 99% increase. The heterologous DHAD specific activity can also be expressed by “fold increase” over control. Thus, the increase in specific activity can be selected from the group consisting of: (a) greater than about 2-fold higher, (b) greater than about 5-fold higher, (c) greater than about 8-fold higher, or (d) greater than about 10-fold higher than control. 
     Fe—S Cluster Forming Proteins and Fe Regulation, Utilization, and Homeostasis 
     As described above, DHAD enzymes require Fe—S clusters for functioning, therefore, they must be expressed in a host having the genetic machinery to produce and load Fe—S clusters into the apo-protein if they are going to be expressed in functional form. As described elsewhere herein, in normal yeast, the mitochondria play an important role in Fe—S cluster biosynthesis. The flux in the formation and movement of Fe—S cluster precursors from mitochondria to Fe—S cluster requiring proteins in the cytosol of normal yeast is believed to be limited. For example, after a point a further increase in the expression of the protein of heterologous DHADs in the cytosol does not result in a corresponding increase in DHAD activity. While not wishing to be bound by theory, it is believed that this is because the increased amounts of the heterologous DHAD are not getting loaded with the Fe—S cluster requisite for activity because the cell is not able to supply the increased demand for Fe—S clusters that arises in the conditions described above. Demonstrated herein is that yeast cells can be genetically modified in 2 ways (separately or contemporaneously) that will result in an increased fraction of the heterologous DHAD expressed in the cytosol being loaded with its requisite Fe—S cluster. One way is to modify the expression of yeast genes involved in the Fe—S cluster formation, such as Fe—S cluster biosynthesis pathway genes or Fe uptake and utilization genes. The other way is to express heterologous genes involved in Fe—S cluster biosynthesis or Fe uptake and utilization in the cytoplasm of yeast. 
     Yeast genes that encode polypeptides that are involved in Fe uptake and utilization and Fe—S cluster biosynthesis are candidates for modification of expression. In embodiments, the modification results in increased function of a selected Fe—S cluster requiring protein. 
     As an example, Aft1 has been found to act as a transcriptional activator for genes into the iron regulon (Kumanovics, et al.  J. Biol. Chem.,  2008. 283, p. 10276-10286; Li, H., et al.,  The Yeast Iron Regulatory Proteins Grx 3/4  and Fra 2  form Heterodimeric Complexes Containing a [ 2 Fe -2 S] Cluster with Cysteinyl and Histidyl Ligation.  Biochemistry, 2009. 48(40): p. 9569-9581. As exemplified herein, the deletion of known inhibitors of Aft1 translocation, results in an increase in specific activity of an Fe—S cluster requiring protein because it leads to an increase Fe—S cluster loading of the protein. While not wishing to be bound by theory, it is thus believed that altering expression of certain genes of the Fe regulon, whether directly or through deletion or upregulation of inhibitors, will likewise increase the loading and function of Fe—S cluster requiring proteins. For example, genes that play a role in, or are part of, Fe utilization and homeostasis in yeast, such as Fe Regulon genes, may be targeted for altered expression. Such genes are known in the art, and examples of these genes are listed in Table 7. (The list in Table 7 is taken from Rutherford, J. C., et al.,  Activation of the Iron Regulon by the Yeast Aft 1 /Aft 2  Transcription Factors Depends on Mitochondrial but Not Cytosolic Iron - Sulfur Protein Biogenesis., J. Biol. Chem.,  2005. 280(11):  p.  10135-10140; Foury, F. and D. Talibi,  Mitochondrial control of iron homeostasis. A genome wide analysis of gene expression in a yeast frataxin - deficient strain. J. Biol. Chem.,  2001. 276(11): p. 7762-7768; and Shakoury-Elizeh, M., et al.,  Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol. Biol. Cell,  2004. 15(3): p. 1233-1243.) 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Examples of yeast genes associated with Fe uptake and utilization. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Nucleic 
                 Amino 
               
               
                   
                   
                 Acid 
                 Acid 
               
               
                 Gene 
                   
                 SEQ ID 
                 SEQ 
               
               
                 Name 
                 Putative Function 
                 NO: 
                 ID NO: 
               
               
                   
               
               
                 ARN1 
                 Transporter, member of the ARN family of transporters that 
                 805 
                 738 
               
               
                   
                 specifically recognize siderophore-iron chelates; responsible 
               
               
                   
                 for uptake of iron bound to ferrirubin, ferrirhodin, and related 
               
               
                   
                 siderophores 
               
               
                 ARN2 
                 Transporter, member of the ARN family of transporters that 
                 806 
                 739 
               
               
                   
                 specifically recognize siderophore-iron chelates; responsible 
               
               
                   
                 for uptake of iron bound to the siderophore 
               
               
                   
                 triacetylfusarinine C 
               
               
                 ATX1 
                 Cytosolic copper metallochaperone that transports copper to 
                 802 
                 735 
               
               
                   
                 the secretory vesicle copper transporter Ccc2p for eventual 
               
               
                   
                 insertion into Fet3p, which is a multicopper oxidase required 
               
               
                   
                 for high-affinity iron uptake 
               
               
                 CCC2 
                 Cu(+2)-transporting P-type ATPase, required for export of 
                 803 
                 736 
               
               
                   
                 copper from the cytosol into an extracytosolic compartment; 
               
               
                   
                 has similarity to human proteins involved in Menkes and 
               
               
                   
                 Wilsons diseases 
               
               
                 COT1 
                 Vacuolar transporter that mediates zinc transport into the 
                 816 
                 749 
               
               
                   
                 vacuole; overexpression confers resistance to cobalt and 
               
               
                   
                 rhodium 
               
               
                 ENB1 
                 Endosomal ferric enterobactin transporter, expressed under 
                 808 
                 741 
               
               
                 (ARN4) 
                 conditions of iron deprivation; member of the major 
               
               
                   
                 facilitator superfamily; expression is regulated by Rcs1p and 
               
               
                   
                 affected by chloroquine treatment 
               
               
                 FET3 
                 Ferro-O2-oxidoreductase required for high-affinity iron 
                 800 
                 733 
               
               
                   
                 uptake and involved in mediating resistance to copper ion 
               
               
                   
                 toxicity, belongs to class of integral membrane multicopper 
               
               
                   
                 oxidases 
               
               
                 FET5 
                 Multicopper oxidase, integral membrane protein with 
                 814 
                 747 
               
               
                   
                 similarity to Fet3p; may have a role in iron transport 
               
               
                 FIT1 
                 Mannoprotein that is incorporated into the cell wall via a 
                 792 
                 725 
               
               
                   
                 glycosylphosphatidylinositol (GPI) anchor, involved in the 
               
               
                   
                 retention of siderophore-iron in the cell wall 
               
               
                 FIT2 
                 Mannoprotein that is incorporated into the cell wall via a 
                 793 
                 726 
               
               
                   
                 glycosylphosphatidylinositol (GPI) anchor, involved in the 
               
               
                   
                 retention of siderophore-iron in the cell wall 
               
               
                 FIT3 
                 Mannoprotein that is incorporated into the cell wall via a 
                 794 
                 727 
               
               
                   
                 glycosylphosphatidylinositol (GPI) anchor, involved in the 
               
               
                   
                 retention of siderophore-iron in the cell wall 
               
               
                 FRE1 
                 Ferric reductase and cupric reductase, reduces siderophore- 
                 795 
                 728 
               
               
                   
                 bound iron and oxidized copper prior to uptake by 
               
               
                   
                 transporters; expression induced by low copper and iron 
               
               
                   
                 levels 
               
               
                 FRE2 
                 Ferric reductase and cupric reductase, reduces siderophore- 
                 796 
                 729 
               
               
                   
                 bound iron and oxidized copper prior to uptake by 
               
               
                   
                 transporters; expression induced by low copper and iron 
               
               
                   
                 levels 
               
               
                 FRE3 
                 Ferric reductase, reduces siderophore-bound iron prior to 
                 797 
                 730 
               
               
                   
                 uptake by transporters; expression induced by low iron levels 
               
               
                 FRE4 
                 Ferric reductase, reduces a specific subset of siderophore- 
                 798 
                 731 
               
               
                   
                 bound iron prior to uptake by transporters; expression 
               
               
                   
                 induced by low iron levels 
               
               
                 FRE5 
                 Putative ferric reductase with similarity to Fre2p; expression 
                 799 
                 732 
               
               
                   
                 induced by low iron levels; the authentic, non-tagged protein 
               
               
                   
                 is detected in highly purified mitochondria in high- 
               
               
                   
                 throughput studies 
               
               
                 FRE6 
                 Putative ferric reductase with similarity to Fre2p; expression 
                 817 
                 750 
               
               
                   
                 induced by low iron levels 
               
               
                 FTH1 
                 Putative high affinity iron transporter involved in transport of 
                 813 
                 746 
               
               
                   
                 intravacuolar stores of iron; forms complex with Fet5p; 
               
               
                   
                 expression is regulated by iron; proposed to play indirect role 
               
               
                   
                 in endocytosis 
               
               
                 FTR1 
                 High affinity iron permease involved in the transport of iron 
                 801 
                 734 
               
               
                   
                 across the plasma membrane; forms complex with Fet3p; 
               
               
                   
                 expression is regulated by iron 
               
               
                 HMX1 
                 ER localized, heme-binding peroxidase involved in the 
                 823 
                 756 
               
               
                   
                 degradation of heme; does not exhibit heme oxygenase 
               
               
                   
                 activity despite similarity to heme oxygenases; expression 
               
               
                   
                 regulated by AFT1 
               
               
                 SIT1 
                 Ferrioxamine B transporter, member of the ARN family of 
                 807 
                 740 
               
               
                 (ARN3) 
                 transporters that specifically recognize siderophore-iron 
               
               
                   
                 chelates; transcription is induced during iron deprivation and 
               
               
                   
                 diauxic shift; potentially phosphorylated by Cdc28p 
               
               
                 SMF3 
                 Putative divalent metal ion transporter involved in iron 
                 815 
                 741 
               
               
                   
                 homeostasis; transcriptionally regulated by metal ions; 
               
               
                   
                 member of the Nramp family of metal transport proteins 
               
               
                 TIS11 
                 mRNA-binding protein expressed during iron starvation; 
                 824 
                 757 
               
               
                 (CTH2) 
                 binds to a sequence element in the 3′-untranslated regions of 
               
               
                   
                 specific mRNAs to mediate their degradation; involved in 
               
               
                   
                 iron homeostasis 
               
               
                 VHT1 
                 High-affinity plasma membrane H+-biotin (vitamin H) 
                 822 
                 755 
               
               
                   
                 symporter; mutation results in fatty acid auxotrophy; 12 
               
               
                   
                 transmembrane domain containing major facilitator 
               
               
                   
                 subfamily member; mRNA levels negatively regulated by 
               
               
                   
                 iron deprivation and biotin 
               
               
                   
               
            
           
         
       
     
     Based on their functions and association with Fe uptake and utilization, the proteins encoded by the genes disclosed in Table 7 are candidates for affecting Fe—S cluster biosynthesis. Additional yeast genes associated with Fe uptake and utilization or Fe—S cluster biosynthesis include those listed in Table 8. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Genes Associated With Yeast Fe Uptake and Utilization or Fe—S Cluster Biosynthesis 
               
            
           
           
               
               
               
               
            
               
                   
                 Nucleic 
                 Amino 
                   
               
               
                   
                 Acid 
                 Acid 
               
               
                 Gene 
                 SEQ ID 
                 SEQ ID 
               
               
                 Name 
                 NO: 
                 NO: 
                 Putative Function 
               
               
                   
               
               
                 AFT1 
                 770 
                 703 
                 Transcription factor involved in iron utilization and homeostasis; binds the 
               
               
                   
                   
                   
                 consensus site PyPuCACCCPu and activates the expression of target genes in 
               
               
                   
                   
                   
                 response to changes in iron availability 
               
               
                 AFT2 
                 771 
                 704 
                 Iron-regulated transcriptional activator; activates genes involved in 
               
               
                   
                   
                   
                 intracellular iron use and required for iron homeostasis and resistance to 
               
               
                   
                   
                   
                 oxidative stress; similar to Aft1p 
               
               
                 AIM1 
                 779 
                 712 
                 Interacts with Grx3/4 
               
               
                 ARH1 
                 855 
                 837 
                 Oxidoreductase of the mitochondrial inner membrane, involved in 
               
               
                   
                   
                   
                 cytoplasmic and mitochondrial iron homeostasis and required for activity of 
               
               
                   
                   
                   
                 Fe—S cluster-containing enzymes; one of the few mitochondrial proteins 
               
               
                   
                   
                   
                 essential for viability (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. 
               
               
                   
                   
                   
                 Biochem. 77: 669-700 (2008)) 
               
               
                 ATM1 
                 830 
                 763 
                 Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, 
               
               
                   
                   
                   
                 exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters 
               
               
                   
                   
                   
                 to the cytosol (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 
               
               
                   
                   
                   
                 77: 669-700 (2008)) 
               
               
                 BUD32 
                 778 
                 711 
                 Interacts with Grx3/4 and Aft1p 
               
               
                 CAD1 
                 791 
                 724 
                 Stress responses including Fe deprivation; also regulates CTI6 and MRS4 
               
               
                 (YAP2) 
                   
                   
                 genes 
               
               
                 CCC1 
                 811 
                 744 
                 Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of 
               
               
                   
                   
                   
                 yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing 
               
               
                   
                   
                   
                 mitochondrial iron accumulation 
               
               
                 CFD1 
                 834 
                 767 
                 Highly conserved, iron-sulfur cluster binding protein localized in the 
               
               
                   
                   
                   
                 cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur 
               
               
                   
                   
                   
                 protein assembly in the cytosol (see, e.g., Lill, R. and U. Muehlenhoff, Ann. 
               
               
                   
                   
                   
                 Rev. Biochem. 77: 669-700 (2008)) 
               
               
                 CIA1 
                 836 
                 769 
                 WD40 repeat protein involved in assembly of cytosolic and nuclear iron- 
               
               
                   
                   
                   
                 sulfur proteins; similar to the human Ciaol protein; YDR267C is an essential 
               
               
                   
                   
                   
                 gene (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 
               
               
                   
                   
                   
                 (2008)) 
               
               
                 CMK1 
                 784 
                 717 
                 Interacts with Grx4p 
               
               
                 CTH1 
                 825 
                 758 
                 mRNA binding and degradation under Fe depletion conditions 
               
               
                 CTI6 
                 786 
                 719 
                 Growth in low iron conditions 
               
               
                 CYC8 
                 787 
                 720 
                 General transcriptional co-repressor, acts together with Tup1p; also acts as 
               
               
                 (SSN6) 
                   
                   
                 part of a transcriptional co-activator complex that recruits the SWI/SNF and 
               
               
                   
                   
                   
                 SAGA complexes to promoters; can form the prion [OCT+] 
               
               
                 DAP1 
                 820 
                 753 
               
               
                 DRE2 
                 781 
                 714 
                 Interacts with Grx3p 
               
               
                 ERV1 
                 856 
                 838 
                 Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space 
               
               
                   
                   
                   
                 (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS 
               
               
                   
                   
                   
                 retention of imported proteins; ortholog of human hepatopoietin (ALR) (see, 
               
               
                   
                   
                   
                 e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 (2008)) 
               
               
                   
                   
                   
                 Central players of the export pathway are the ABC transporter Atm1p of the 
               
               
                   
                   
                   
                 mitochondrial inner membrane, the sulfhydryl oxidase Erv1p of the 
               
               
                   
                   
                   
                 intermembrane space, and the tripeptide glutathione (23, 27, 50) (see Gerber, 
               
               
                   
                   
                   
                 J., et al.,  Mol. Cell. Biol. 24 (11): 4848-57 (2004)) 
               
               
                 ESA1 
                 782 
                 715 
                 Interacts with Grx4p/Aft1p 
               
               
                 FET4 
                 809 
                 742 
                 Low-affinity Fe(II) transporter of the plasma membrane 
               
               
                 FRA1 
                 772 
                 705 
                 Protein involved in negative regulation of transcription of iron regulon; forms 
               
               
                   
                   
                   
                 an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; 
               
               
                   
                   
                   
                 mutant fails to repress transcription of iron regulon and is defective in spore 
               
               
                   
                   
                   
                 formation 
               
               
                 FRA2 
                 773 
                 706 
                 Protein involved in negative regulation of transcription of iron regulon; forms 
               
               
                   
                   
                   
                 an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails 
               
               
                   
                   
                   
                 to repress iron regulon and is sensitive to nickel 
               
               
                 GEF1 
                 804 
                 737 
                 Copper transporter/loading for Fet3p 
               
               
                 GGC1 
                 857 
                 839 
                 Mitochondrial GTP/GDP transporter, essential for mitochondrial genome 
               
               
                 (YHM1) 
                   
                   
                 maintenance; has a role in mitochondrial iron transport; member of the 
               
               
                   
                   
                   
                 mitochondrial carrier family 
               
               
                 GRX1 
                 858 
                 840 
                 Hydroperoxide and superoxide-radical responsive heat-stable glutathione- 
               
               
                   
                   
                   
                 dependent disulfide oxidoreductase with active site cysteine pair; protects 
               
               
                   
                   
                   
                 cells from oxidative damage 
               
               
                 GRX2 
                 832 
                 765 
                 Cytoplasmic glutaredoxin, thioltransferase, glutathione-dependent disulfide 
               
               
                   
                   
                   
                 oxidoreductase involved in maintaining redox state of target proteins, also 
               
               
                   
                   
                   
                 exhibits glutathione peroxidase activity, expression induced in response to 
               
               
                   
                   
                   
                 stress 
               
               
                 GRX3 
                 774 
                 707 
                 Hydroperoxide and superoxide-radical responsive glutathione-dependent 
               
               
                   
                   
                   
                 oxidoreductase; monothiol glutaredoxin subfamily member along with Grx4p 
               
               
                   
                   
                   
                 and Grx5p; protects cells from oxidative damage 
               
               
                 GRX4 
                 775 
                 708 
                 Hydroperoxide and superoxide-radical responsive glutathione-dependent 
               
               
                   
                   
                   
                 oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p 
               
               
                   
                   
                   
                 and Grx5p; protects cells from oxidative damage. 
               
               
                 GRX5 
                 831 
                 764 
                 Hydroperoxide and superoxide-radical responsive glutathione-dependent 
               
               
                   
                   
                   
                 oxidoreductase; mitochondrial matrix protein involved in the 
               
               
                   
                   
                   
                 synthesis/assembly of iron-sulfur centers; monothiol glutaredoxin subfamily 
               
               
                   
                   
                   
                 member along with Grx3p and Grx4p (see, e.g., Lill, R. and U. Muehlenhoff, 
               
               
                   
                   
                   
                 Ann. Rev. Biochem. 77: 669-700 (2008)) 
               
               
                 HDA1 
                 790 
                 723 
                 Interacts with Tup1p, Ssn6p for Aft1/2p regulation in the absence of heme 
               
               
                 IBA57 
                 859 
                 841 
                 Mitochondrial matrix protein involved in the incorporation of iron-sulfur 
               
               
                   
                   
                   
                 clusters into mitochondrial aconitase-type proteins; activates the radical-SAM 
               
               
                   
                   
                   
                 family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid 
               
               
                   
                   
                   
                 system (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 
               
               
                   
                   
                   
                 (2008)) 
               
               
                 ISA1 
                 860 
                 842 
                 Mitochondrial matrix protein involved in biogenesis of the iron-sulfur (Fe/S) 
               
               
                   
                   
                   
                 cluster of Fe/S proteins, isa1 deletion causes loss of mitochondrial DNA and 
               
               
                   
                   
                   
                 respiratory deficiency; depletion reduces growth on nonfermentable carbon 
               
               
                   
                   
                   
                 sources (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 
               
               
                   
                   
                   
                 (2008)) 
               
               
                 ISA2 
                 861 
                 843 
                 Protein required for maturation of mitochondrial and cytosolic Fe/S proteins, 
               
               
                   
                   
                   
                 localizes to the mitochondrial intermembrane space, overexpression of ISA2 
               
               
                   
                   
                   
                 suppresses grx5 mutations (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. 
               
               
                   
                   
                   
                 Biochem. 77: 669-700 (2008)) 
               
               
                 ISU1 
                 828 
                 761 
                 Conserved protein of the mitochondrial matrix, performs a scaffolding 
               
               
                   
                   
                   
                 function during assembly of iron-sulfur clusters, interacts physically and 
               
               
                   
                   
                   
                 functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable 
               
               
                   
                   
                   
                 (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 
               
               
                   
                   
                   
                 (2008)) 
               
               
                 ISU2 
                 829 
                 762 
                 Conserved protein of the mitochondrial matrix, required for synthesis of 
               
               
                   
                   
                   
                 mitochondrial and cytosolic iron-sulfur proteins, performs a scaffolding 
               
               
                   
                   
                   
                 function in mitochondria during Fe/S cluster assembly; isu1 isu2 double 
               
               
                   
                   
                   
                 mutant is inviable (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. 
               
               
                   
                   
                   
                 Biochem. 77: 669-700 (2008)) 
               
               
                 JAC1 
                 862 
                 844 
                 Specialized J-protein that functions with Hsp70 in Fe—S cluster biogenesis in 
               
               
                   
                   
                   
                 mitochondria, involved in iron utilization; contains a J domain typical to J- 
               
               
                   
                   
                   
                 type chaperones; localizes to the mitochondrial matrix (see, e.g., Lill, R. and 
               
               
                   
                   
                   
                 U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 (2008)) 
               
               
                 MGE1 
                 863 
                 845 
                 Mitochondrial matrix cochaperone, acts as a nucleotide release factor for 
               
               
                   
                   
                   
                 Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p 
               
               
                   
                   
                   
                 in folding of Fe—S cluster proteins; homolog of  E. coli  GrpE (see, e.g., Lill, R. 
               
               
                   
                   
                   
                 and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 (2008)) 
               
               
                 MRS3 
                 819 
                 752 
                 Iron transporter that mediates Fe2+ transport across the inner mitochondrial 
               
               
                   
                   
                   
                 membrane; mitochondrial carrier family member, similar to and functionally 
               
               
                   
                   
                   
                 redundant with Mrs4p; active under low-iron conditions; may transport other 
               
               
                   
                   
                   
                 cations (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 
               
               
                   
                   
                   
                 (2008)) 
               
               
                 MRS4 
                 818 
                 751 
                 Iron transporter that mediates Fe2+ transport across the inner mitochondrial 
               
               
                   
                   
                   
                 membrane; mitochondrial carrier family member, similar to and functionally 
               
               
                   
                   
                   
                 redundant with Mrs3p; active under low-iron conditions; may transport other 
               
               
                   
                   
                   
                 cations (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 
               
               
                   
                   
                   
                 (2008)) 
               
               
                 MSN5 
                 776 
                 709 
                 Exporting Aft1p and other proteins from the nucleus 
               
               
                 NAR1 
                 833 
                 766 
                 Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, 
               
               
                   
                   
                   
                 required for maturation of cytosolic and nuclear FeS proteins and for normal 
               
               
                   
                   
                   
                 resistance to oxidative stress; homologous to human Narf (see, e.g., Lill, R. 
               
               
                   
                   
                   
                 and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 (2008)) 
               
               
                 NBP35 
                 835 
                 768 
                 Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms 
               
               
                   
                   
                   
                 a complex with Cfd1p that is involved in iron-sulfur protein assembly in the 
               
               
                   
                   
                   
                 cytosol; similar to P-loop NTPases (see, e.g., Lill, R. and U. Muehlenhoff, 
               
               
                   
                   
                   
                 Ann. Rev. Biochem. 77: 669-700 (2008)) 
               
               
                 NFS1 
                 864 
                 846 
                 Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis; 
               
               
                   
                   
                   
                 required for the post-transcriptional thio-modification of mitochondrial and 
               
               
                   
                   
                   
                 cytoplasmic tRNAs; essential protein located predominantly in mitochondria 
               
               
                   
                   
                   
                 (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 77: 669-700 
               
               
                   
                   
                   
                 (2008)) 
               
               
                 NFU1 
                 865 
                 847 
                 Protein involved in iron utilization in mitochondria; similar to NifU, which is 
               
               
                   
                   
                   
                 a protein required for the maturation of the Fe/S clusters of nitrogenase in 
               
               
                   
                   
                   
                 nitrogen-fixing bacteria (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. 
               
               
                   
                   
                   
                 Biochem. 77: 669-700 (2008)) 
               
               
                 NHP6a 
                 788, 789 
                 721, 722 
                 Both are high-mobility group non-histone chromatin protein, functionally 
               
               
                 and b 
                   
                   
                 redundant with Nhp6Bp; homologous to mammalian high mobility group 
               
               
                   
                   
                   
                 proteins 1 and 2; acts to recruit transcription factor Rcs1p to certain 
               
               
                   
                   
                   
                 promoters 
               
               
                 PSE1 
                 777 
                 710 
                 Importing Aft1p and other proteins to the nucleus 
               
               
                 SMF1 
                 810 
                 743 
                 Low affinity Fe(II) transporter of the plasma membrane 
               
               
                 SNF1 
                 866 
                 848 
                 AMP-activated serine/threonine protein kinase found in a complex containing 
               
               
                   
                   
                   
                 Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for 
               
               
                   
                   
                   
                 transcription of glucose-repressed genes, thermotolerance, sporulation, and 
               
               
                   
                   
                   
                 peroxisome biogenesis 
               
               
                 SNF2 
                 867 
                 849 
                 Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in 
               
               
                   
                   
                   
                 transcriptional regulation; contains DNA-stimulated ATPase activity; 
               
               
                   
                   
                   
                 functions interdependently in transcriptional activation with Snf5p and Snf6p 
               
               
                 SNF3 
                 868 
                 850 
                 Plasma membrane glucose sensor that regulates glucose transport; has 12 
               
               
                   
                   
                   
                 predicted transmembrane segments; long cytoplasmic C-terminal tail is 
               
               
                   
                   
                   
                 required for low glucose induction of hexose transporter genes HXT2 and 
               
               
                   
                   
                   
                 HXT4 
               
               
                 SNF4 
                 869 
                 851 
                 Activating gamma subunit of the AMP-activated Snf1p kinase complex 
               
               
                   
                   
                   
                 (contains Snf1p and a Sip1p/Sip2p/Gal83p family member); activates 
               
               
                   
                   
                   
                 glucose-repressed genes, represses glucose-induced genes; role in sporulation, 
               
               
                   
                   
                   
                 and peroxisome biogenesis 
               
               
                 SSQ1 
                 827 
                 760 
                 Mitochondrial hsp70-type molecular chaperone, required for assembly of 
               
               
                   
                   
                   
                 iron/sulfur clusters into proteins at a step after cluster synthesis, and for 
               
               
                   
                   
                   
                 maturation of Yfh1p, which is a homolog of human frataxin implicated in 
               
               
                   
                   
                   
                 Friedreich&#39;s ataxia (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. 
               
               
                   
                   
                   
                 Biochem. 77: 669-700 (2008)) 
               
               
                 TIM12 
                 871 
                 853 
                 Essential protein of the inner mitochondrial membrane, peripherally 
               
               
                 (MRS5) 
                   
                   
                 localized; component of the TIM22 complex, which is a twin-pore translocase 
               
               
                   
                   
                   
                 that mediates insertion of numerous multispanning inner membrane protein. 
               
               
                 TUP1 
                 785 
                 718 
                 General repressor of transcription 
               
               
                 NP_011911.1 
                 821 
                 754 
               
               
                 VPS41 
                 872 
                 854 
                 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion 
               
               
                 (FET2) 
                   
                   
                 and vacuole protein sorting (HOPS) complex; essential for membrane 
               
               
                   
                   
                   
                 docking and fusion at the Golgi-to-endosome and endosome-to-vacuole 
               
               
                   
                   
                   
                 stages of protein transport 
               
               
                 YAH1 
                 870 
                 852 
                 Ferredoxin of the mitochondrial matrix required for formation of cellular 
               
               
                   
                   
                   
                 iron-sulfur proteins; involved in heme A biosynthesis; homologous to human 
               
               
                   
                   
                   
                 adrenodoxin (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 
               
               
                   
                   
                   
                 77: 669-700 (2008)) 
               
               
                 YAP5 
                 812 
                 745 
                 Regulation (CCC1) 
               
               
                 YFH1 
                 826 
                 759 
                 Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with 
               
               
                 (Frataxin) 
                   
                   
                 Isu1p to promote Fe—S cluster assembly; mutation results in multiple Fe/S- 
               
               
                   
                   
                   
                 dependent enzyme deficiencies; human frataxin homolog is mutated in 
               
               
                   
                   
                   
                 Friedrich&#39;s ataxia (see, e.g., Lill, R. and U. Muehlenhoff, Ann. Rev. Biochem. 
               
               
                   
                   
                   
                 77: 669-700 (2008)) 
               
               
                 YRA1 
                 783 
                 716 
                 Interacts with Grx4p 
               
               
                 ZPR1 
                 780 
                 713 
                 Interacts with Aft1p 
               
               
                   
               
            
           
         
       
     
     Additional genes encoding polypeptides affecting Fe—S cluster biosynthesis from other host cells have been identified and include, but are not limited to, those genes listed in Table 9. 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Genes Directly Involved in Fe—S Cluster Biosynthesis from Various Cells 
               
            
           
           
               
               
            
               
                 Gene Name 
                   
               
               
                 SEQ ID 
               
               
                 NOs(Amino 
               
               
                 Acid, Nucleic 
                 Function 
               
               
                 Acid) 
                 (Accession; CDS) 
               
               
                   
               
            
           
           
               
            
               
                   Azotobacter vinelandii  nif genes 
               
               
                 (FIGS. 6A and 6B; see Johnson, D. C., et al.,  Ann. Rev. Biochem. 74 : 247-81 (2005)) 
               
            
           
           
               
               
            
               
                 iscA nif   
                 [Fe—S] cluster scaffold protein (see Johnson, D. C., et al.,  Ann. Rev.   
               
               
                 (873, 894) 
                   Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002797399.1; nucleotides 153037 to 153360 of NC_012560.1) 
               
               
                 nifU 
                 NifU is a scaffold protein for assembly and transfer of iron-sulfur 
               
               
                 (875, 896) 
                 clusters (see Johnson, D. C., et al.,  Ann. Rev. Biochem. 74 : 247-81 
               
               
                   
                 (2005)). 
               
               
                   
                 (YP_002797400.1; nucleotides 153425 to 154363 of NC_012560.1) 
               
               
                 nifS 
                 Cysteine desulfurase involved in the mobilization of S for nitrogenase 
               
               
                 (874, 895) 
                 maturation (see Johnson, D. C., et al.,  Ann. Rev. Biochem. 74 : 247-81 
               
               
                   
                 (2005)). 
               
               
                   
                 (YP_002797401.1; nucleotides 154365 to 155573 of NC_012560.1) 
               
               
                 cysE1 
                 Involved in cysteine biosynthesis (see Johnson, D. C., et al.,  Ann. Rev.   
               
               
                 (876, 897) 
                   Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002797403.1; nucleotides 156797 to 157594 of NC_012560.1) 
               
               
                 cysE2 
                 Involved in cysteine biosynthesis (see Johnson, D. C., et al.,  Ann. Rev.   
               
               
                 (929, 947) 
                   Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002801153.1; reverse complement of nucleotides 4092159 to 
               
               
                   
                 4092938 of NC_012560.1) 
               
               
                 iscS 
                 Cysteine desulfurase involved in the mobilization of S (see Johnson, D. C., 
               
               
                 (930, 948) 
                 et al.,  Ann. Rev. Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002801151.1; reverse complement of nucleotides of 4090290 to 
               
               
                   
                 4091504 of NC_012560.1) 
               
               
                 iscU 
                 [Fe—S] cluster scaffold protein (see Johnson, D. C., et al.,  Ann. Rev.   
               
               
                 (931, 949) 
                   Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002801150.1; reverse complement of nucleotides 4089860 to 
               
               
                   
                 4090246 of NC_012560.1) 
               
               
                 iscA 
                 [Fe—S] cluster scaffold protein (see Johnson, D. C., et al.,  Ann. Rev.   
               
               
                 (932, 950) 
                   Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002801149.1; reverse complement of nucleotides 4089511 to 
               
               
                   
                 4089834 of NC_012560.1) 
               
               
                 hscB 
                 HscB heat shock cognate protein associated with Isc-directed [Fe—S] 
               
               
                 (933, 951) 
                 protein maturation (see Johnson, D. C., et al.,  Ann. Rev. Biochem.   
               
               
                   
                   74 : 247-81 (2005)) 
               
               
                   
                 (YP_002801148.1; reverse complement of nucleotides 4088980 to 
               
               
                   
                 4089501 of NC_012560.1) 
               
               
                 hscA 
                 HscA heat shock cognate protein associated with Isc-directed [Fe—S] 
               
               
                 (934, 952) 
                 protein maturation (see Johnson, D. C., et al.,  Ann. Rev. Biochem.   
               
               
                   
                   74 : 247-81 (2005)) 
               
               
                   
                 (YP_002801147.1; reverse complement of nucleotides 4087072 to 
               
               
                   
                 4088937 of NC_012560.1) 
               
               
                 Fdx 
                 Ferredoxin 
               
               
                 (935, 953) 
                 (YP_002801146.1; reverse complement of nucleotides 4086730 to 
               
               
                   
                 4087071 of NC_012560.1) 
               
               
                 sufS 
                 Cysteine desulfurase involved in the mobilization of S (see Johnson, D. C., 
               
               
                 (936, 954) 
                 et al.,  Ann. Rev. Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002801025.1; nucleotides 3961166 to 3962515 of NC_012560.1) 
               
               
                 sufE 
                 (YP_002801026.1; nucleotides 3962512 to 3962916 of NC_012560.1) 
               
               
                 (937, 955) 
               
               
                 cysE3 
                 Involved in cysteine biosynthesis (see Johnson, D. C., et al.,  Ann. Rev.   
               
               
                 (938, 956) 
                   Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002799274.1; nucleotides 2093069 to 2094052 of NC_012560.1) 
               
               
                 sufS2 
                 Cysteine desulfurase involved in the mobilization of S (see Johnson, D. C., 
               
               
                 (939, 957) 
                 et al.,  Ann. Rev. Biochem. 74 : 247-81 (2005)) 
               
               
                   
                 (YP_002799276.1; nucleotides 2095267 to 2097081 of NC_012560.1) 
               
               
                 iscA2 also 
                 [Fe—S] cluster scaffold protein (see Johnson, D. C., et al.,  Ann. Rev.   
               
               
                 known as 
                   Biochem. 74 : 247-81 (2005)) 
               
               
                 eprA 
                 (YP_002801687.1; reverse complement of nucleotides 4681573 to 
               
               
                 (877, 898) 
                 4681923 of NC_012560.1) 
               
               
                 Nfu also 
                 Human nfu appears to be a persulfide reductase according to the 
               
               
                 known as 
                 equation shown in FIG. 6C. (see Liu, Y., W. Qi, and J. A. Cowan, 
               
               
                 NfuA 
                   Biochem.  48(5): 973-80 (2009)) 
               
               
                 (878, 899) 
                 (YP_002800022.1; reverse complement of nucleotides 2961161 to 
               
               
                   
                 2961745 of NC_012560.1) 
               
               
                 nfuA also 
                 Spectroscopic and analytical studies indicate that one 
               
               
                 known as 
                 [4Fe—4S] cluster can be assembled in vitro within a dimeric form 
               
               
                 AnfU 
                 of NfuA. The resultant [4Fe—4S] cluster-loaded form of NfuA is 
               
               
                 (879, 900) 
                 competent for rapid in vitro activation of apo-aconitase. Based 
               
               
                   
                 on these results a model is proposed where NfuA could represent 
               
               
                   
                 a class of intermediate [Fe—S] cluster carriers involved in 
               
               
                   
                 [Fe—S] protein maturation. (see Bandyopadhyay, S., et al.,  J Biol. Chem.   
               
               
                   
                 283(20): 14092-99 (2008)) 
               
               
                   
                 (YP_002801977.1; nucleotides 4963727 to 4964017 of NC_012560.1) 
               
               
                 nfuV also 
                 Could have specialized functions related to the maturation, protection, 
               
               
                 known as 
                 or repair of specific [Fe—S] proteins (see Johnson, D. C., et al.,  Ann. Rev.   
               
               
                 VnfU 
                   Biochem. 74 : 247-81 (2005)). 
               
               
                 (880, 901) 
                 (YP_002797514.1; reverse complement of nucleotides 263828 to 
               
               
                   
                 264118 of NC_012560.1) 
               
            
           
           
               
            
               
                   Helicobacter pylori  nif genes 
               
               
                 (FIG. 7; see Johnson, D. C., et al.,  Ann. Rev. Biochem. 74 : 247-81 (2005)) 
               
            
           
           
               
               
            
               
                 nifS 
                 NifS is a cysteine desulfurase. 
               
               
                 (881, 902) 
                 (YP_003057033.1; nucleotides 218891 to 220054 of NC_012973.1) 
               
               
                 nifU 
                 NifU is a scaffold protein for assembly and transfer of iron-sulfur 
               
               
                 (882, 903) 
                 clusters. 
               
               
                   
                 (YP_003057034.1; nucleotides 220076 to 221056 of NC_012973.1) 
               
               
                 nfu 
                 (YP_003058109.1; nucleotides 1448886 to 1449155 of NC_012973.1) 
               
               
                 (927, 945) 
               
               
                 iscS 
                 (YP_003057709.1; reverse complement of nucleotides 1012615 to 
               
               
                 (928, 946) 
                 1013937 of NC_012973.1) 
               
            
           
           
               
            
               
                   E. coli  isc genes 
               
               
                 (FIG. 8; see Johnson, D. C., et al.,  Ann. Rev. Biochem. 74 : 247-81 (2005)) 
               
            
           
           
               
               
            
               
                 iscS 
                 EcoCyc: IscS is a cysteine desulfurase that catalyzes the conversion of 
               
               
                 (883, 904) 
                 cysteine into alanine and sulfur via intermediate formation of a cysteine 
               
               
                   
                 persulfide. 
               
               
                   
                 (YP_026169.1; reverse complement of nucleotides 2658339 to 2659553 
               
               
                   
                 of NC_000913.2) 
               
               
                 iscU 
                 EcoCyc: IscU is a scaffold protein for assembly and transfer of iron- 
               
               
                 (884, 905) 
                 sulfur clusters. IscU is able to form 2Fe—2S clusters and transfer them to 
               
               
                   
                 apo-ferredoxin, acting catalytically. The chaperones HscA and HscB 
               
               
                   
                 and ATP hydrolysis by HscA accelerate cluster transfer. 
               
               
                   
                 (NP_417024.1; reverse complement of nucleotides 2657925 to 2658311 
               
               
                   
                 of NC_000913.2) 
               
               
                 iscA 
                 EcoCyc: IscA is an iron-sulfur cluster assembly protein that forms the 
               
               
                 (885, 906) 
                 [2Fe—2S] cluster of ferredoxin. It has been shown to bind iron with an 
               
               
                   
                 apparent association constant of 3 × 10−19M −1 . In vitro in the presence of 
               
               
                   
                 IscS and cysteine, IscA can provide iron to iscU. 
               
               
                   
                 Native [2Fe—2S] SufA can transfer its Fe—S cluster to both [2Fe—2S] and 
               
               
                   
                 [4Fe—4S] apoproteins. (see Gupta, V., et al.,  J. Am. Chem. Soc.   
               
               
                   
                 131(17): 6149-53 (2009)) 
               
               
                   
                 The results suggest that the biogenesis of the [4Fe—4S] clusters and the 
               
               
                   
                 [2Fe—2S] clusters may have distinct pathways and that IscA/SufA 
               
               
                   
                 paralogues are essential for the [4Fe—4S] cluster assembly, but are 
               
               
                   
                 dispensable for the [2Fe—2S] cluster assembly in  E. coli  under aerobic 
               
               
                   
                 conditions. (Tan, G., et al.,  Biochem. J. , 420(3): 463-72 (2009)) 
               
               
                   
                 (NP_417023.1; reverse complement of nucleotides 2657585 to 2657908 
               
               
                   
                 of NC_000913.2) 
               
               
                 hscB 
                 EcoCyc: HscB is a co-chaperone that stimulates HscA (Hsc66) ATPase 
               
               
                 (886, 907) 
                 activity. HscB does not exhibit its own chaperone activity. HscB is 
               
               
                   
                 required for wild-type stimulation of HscA ATPase activity by the 
               
               
                   
                 substrate, IscU, and for wild-type interaction between HscA and IscU. 
               
               
                   
                 This system is involved in iron-sulfur cluster assembly. 
               
               
                   
                 (NP_417022.1; reverse complement of nucleotides 2656974 to 2657489 
               
               
                   
                 of NC_000913.2) 
               
               
                 hscA 
                 EcoCyc: Hsc66 together with Hsc20 may comprise a chaperone system 
               
               
                 (887, 908) 
                 similar to DnaK/DnaJ. Hsc66 is required for the assembly of iron-sulfur 
               
               
                   
                 clusters. IscU may be a substrate for Hsc66. In the presence of Hsc20, 
               
               
                   
                 IscU stimulates the ATPase activity of Hsc66 up to 480-fold; the in vivo 
               
               
                   
                 turnover rate of the chaperone cycle may be determined by the 
               
               
                   
                 availability of the IscU-Hsc20 complex. Hsc66 directly interacts with 
               
               
                   
                 IscU, IscA, and Fdx. 
               
               
                   
                 (NP_417021.1; reverse complement of nucleotides 2655107 to 2656957 
               
               
                   
                 of NC_000913.2) 
               
               
                 Fdx 
                 EcoCyc: [2Fe—2S] ferridoxin 
               
               
                 (888, 909) 
                 (NP_417020.1; reverse complement of nucleotides 2654770 to 2655105 
               
               
                   
                 of NC_000913.2) 
               
            
           
           
               
            
               
                   E. coli  suf genes 
               
               
                 (FIG. 9; see Johnson, D. C., et al.,  Ann. Rev. Biochem. 74 : 247-81 (2005)) 
               
            
           
           
               
               
            
               
                 sufA 
                 EcoCyc: SufA is part of the protein machinery that is involved in the 
               
               
                 (889, 910) 
                 biosynthesis of iron-sulfur clusters. In vitro, purified apoSufA can 
               
               
                   
                 chelate iron-sulfur clusters by treatment with iron and sulfide under 
               
               
                   
                 anaerobic conditions. HoloSufA then can form a fast and tight 
               
               
                   
                 association with the target apoprotein biotin synthase (BioB) and 
               
               
                   
                 transfers a [4Fe—4S] cluster to BioB in a slow reaction. 
               
               
                   
                 (NP_416199.1; reverse complement of nucleotides 1762042 to 1762410 
               
               
                   
                 of NC_000913.2) 
               
               
                 sufB 
                 EcoCyc: The SufB-SufC-SufD complex activates the cysteine 
               
               
                 (890, 911) 
                 desulfurase activity SufS in conjunction with the SufE sulfur acceptor 
               
               
                   
                 protein. 
               
               
                   
                 (NP_416198.2; reverse complement of nucleotides 1760546 to 1762033 
               
               
                   
                 of NC_000913.2) 
               
               
                 sufC 
                 EcoCyc: SufC is part of the protein machinery that is involved in the 
               
               
                 (891, 912) 
                 biosynthesis of iron-sulfur clusters. The SufB-SufC-SufD complex 
               
               
                   
                 activates the cysteine desulfurase activity of SufS in conjunction with 
               
               
                   
                 the SufE sulfur acceptor protein. 
               
               
                   
                 (NP_416197.1; reverse complement of nucleotides 1759790 to 1760536 
               
               
                   
                 of NC_000913.2) 
               
               
                 sufD 
                 EcoCyc: The SufB-SufC-SufD complex activates the cysteine 
               
               
                 (892, 913) 
                 desulfurase activity SufS in conjunction with the SufE sulfur acceptor 
               
               
                   
                 protein 
               
               
                   
                 (NP_416196.1; reverse complement of nucleotides 1758544 to 1759815 
               
               
                   
                 of NC_000913.2) 
               
               
                 sufS 
                 EcoCyc: SufS is a member of the NifS protein family. SufS exhibits 
               
               
                 (893, 914) 
                 activity with respect to assembly of the ferredoxin iron-sulfur cluster in 
               
               
                   
                 an in vitro assay. 
               
               
                   
                 (NP_416195.1; reverse complement of nucleotides 1757327 to 1758547 
               
               
                   
                 of NC_000913.2) 
               
               
                 sufE1 also 
                 (NP_416194.1; reverse complement of nucleotides 1756898 to 1757314 
               
               
                 known as suf E 
                 of NC_000913.2) 
               
               
                 (925, 943) 
               
               
                 sufS2 also 
                 (NP_417290.1; NC_000913.2 nucleotides 2941359 to 2942564) 
               
               
                 known as csdA 
               
               
                 (924, 942) 
               
               
                 sufE2 also 
                 (NP_417291.1; nucleotides 2942564 to 2943007 of NC_000913.2) 
               
               
                 known as csdE 
               
               
                 (926, 944) 
               
               
                 iscA2 also 
                 (NP_414698.1; nucleotides 176610 to 176954 of NC_000913.2) 
               
               
                 known as erpA 
               
               
                 (922, 940) 
               
               
                 nfu also known 
                 (NP_417873.1; nucleotides 3543646 to 3544221 of NC_000913.2) 
               
               
                 as nfuA 
               
               
                 (923, 941) 
               
               
                   
               
            
           
         
       
     
     Fe uptake and metabolism and/or Fe—S cluster biosynthesis genes, including, but not limited to, those listed in Tables 7, 8 or 9 can potentially be deleted, mutated, expressed, up-regulated, or down-regulated to increase the flux in an Fe—S cluster biosynthesis pathway and improve specific activity of Fe—S cluster requiring proteins such as DHAD. In addition, co-factors can be added to change the activity of polypeptides having Fe—S cluster regulatory activity to increase the flux in an Fe—S cluster biosynthesis pathway and improve DHAD specific activity. 
     For example, the genes that increase the flux in an Fe—S cluster biosynthesis pathway can be expressed to improve the activity of DHAD by providing an adequate amount of Fe—S clusters for the apo-enzyme. Any gene, or a combination of them, such as one or more genes listed in Tables 7, 8, or 9, can be cloned and expressed in a pRS411 plasmid as described in Example 4. The resulting constructs, along with the DHAD expression vector pHR81 FBA ilvD(Sm), can then be transformed into wild-type BY4741. As a control, pRS411 without any gene of interest and vector pHR81 FBA ilvD(Sm) are transformed into a wild-type strain. The transformants are selected on agar plates with SD medium without uracil and methionine to maintain both plasmids as described in Example 4. Enzymatic activity for DHAD in the crude extract of different strains from the transformation can be measured. The results can be compared with the specific activity obtained from the control pRS411 without any gene of interest and vector pHR81 FBA ilvD(Sm) transformed into a wild-type strain. An increase in specific activity indicates a gene that can be used to increase the flux in an Fe—S cluster biosynthesis pathway. 
     In addition, strains with deletions in more than one of the genes involved in Fe—S cluster regulatory activity can be created to provide additive effects in improving the enzymes or proteins containing Fe—S cluster(s). For example, double mutants with deletions in both FRA2 and GXR3 genes can be used to transform vector pHR81 FBA-IlvD(sm), and the DHAD activity in the crude extract from the transformants can be measured. 
     Another alternative is to alter the expression of, e.g., the PSE1 (SEQ ID NO:777) gene, which encodes a protein involved in the import of Aft1p into the nucleus (Fukunaka, et al, 2003 , J. Biological Chem ., vol. 278, pp. 50120-50127). Expression of this gene can be accomplished by cloning it in vector pRS411 as described above. 
     Thus, provided herein are recombinant host cells that comprise an alteration in the expression of any polypeptide encoded by an Fe uptake and utilization or an Fe—S cluster biosynthesis gene. Encompassed are recombinant host cells that comprise at least one heterologous polynucleotide of any one of the above-referenced Fe—S cluster biosynthesis genes. Also encompassed are recombinant host cells, wherein the host cell comprises at least one deletion, mutation, and/or substitution in an endogenous gene of any one of the above-referenced Fe uptake and utilization or Fe—S cluster biosynthesis genes. Also provided are recombinant host cells that comprise at least one heterologous polynucleotide of any one of the above-referenced Fe uptake and utilization or Fe—S cluster biosynthesis genes, wherein the host cell comprises at least one deletion, mutation, and/or substitution in an endogenous gene of any one of the above-referenced Fe uptake and utilization or Fe—S cluster biosynthesis genes. 
     These recombinant host cells can also comprise at least one heterologous Fe—S cluster requiring protein. For example, provided herein is a recombinant host cell comprising at least one heterologous DHAD and at least one heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis. Also provided is a recombinant host cell comprising at least one heterologous DHAD, wherein the host cell comprises at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting Fe—S cluster biosynthesis. Also provided is a recombinant host cell comprising at least one heterologous DHAD and at least one heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis, wherein the host cell comprises at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting Fe—S cluster biosynthesis. 
     Host cells that can be used in the present invention include yeast host cells including, but not limited to,  Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia , and  Pichia . Bacterial host cells can also be used to create recombinant host cells that comprise at least one heterologous polynucleotide encoding a polypeptide having DHAD activity and at least one heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis. For example, lactic acid bacteria comprising recombinant DHAD and at least one recombinant genetic expression element encoding Fe—S cluster forming proteins are the subject of U.S. application Ser. No. 12/569,103, filed Sep. 29, 2009, which is incorporated by reference herein. The present recombinant host cells comprising at least one heterologous polynucleotide encoding a polypeptide having DHAD activity and at least one heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis do not include those lactic acid bacteria described in U.S. application Ser. No. 12/569,103, filed Sep. 29, 2009, which is incorporated by reference herein. 
     The polypeptide affecting Fe—S cluster biosynthesis can be selected from the group consisting of the Fe uptake and utilization or Fe—S cluster biosynthesis pathway genes in Tables 7, 8 and 9. In one embodiment, the polypeptide affecting Fe—S cluster biosynthesis is encoded by ARN1, ARN2, ATX1, CCC2, COT1, ENB1, FET3, FET5, FIT1, FIT2, FIT3, FRE1, FRE2, FRE3, FRE4, FRE5, FREE, FTH1, FTR1, HMX1, SIT1, SMF3, TIS11, VHT1, AFT1, AFT2, AIM1, ARH1, ATM1, BUD32, CAD1, CCC1, CFD1, CIA1, CMK1, CTH1, CTI6, CYC8, DAP1, DRE2, ERV1, ESA1, FET4, FRA1, FRA2, GEF1, GGC1, GRX1, GRX2, GRX4, GRX5, HDA1, IBA57, ISA1, ISA2, ISU1, ISU2, JAC1, MGE1, MRS3, MRS4, MSN5, NAR1, NFS1, NFU1, NHP6a, NHP6b, PSE1, SMF1, SNF1, SNF2, SNF3, SNF4, SSQ1, TIM12, TUP1, NP_011911.1, VPS41, YAP5, YFH1, YRA1, ZPR1, iscA nif , nifU, nifS, cysE1, cysE2, iscS, iscU, iscA, hscB, hscA, Fdx, sufS, sufE, cysE3, sufS2, iscA2, Nfu, nfuA, nfuV, nfu, sufA, sufB, sufC, sufD, sufE1, sufS2, or sufE2. In one embodiment, the polypeptide affecting Fe—S cluster biosynthesis is AFT1, AFT2, PSE1, FRA2, GRX3, or MSN5. In one embodiment, the polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of AFT1, AFT2, PSE1, FRA2, GRX3, MSN5, and combinations thereof. In one embodiment, the polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of AFT1, AFT2, PSE1, FRA2, MSN5, and combinations thereof. In another embodiment, the polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of AFT1, AFT2, PSE1, FRA2, GRX3, MSN5, and combinations thereof, and the polypeptide affecting Fe—S cluster biosynthesis is encoded by a polynucleotide comprising a plasmid. In some embodiments, DHAD is co-expressed with AFT1, AFT2, PSE1, and combinations thereof. The polypeptide affecting Fe—S cluster biosynthesis may be a constitutive mutant, such as, but not limited to, AFT1 L99A, AFT1 L102A, AFT1 C291F, AFT1 C293F, and combinations thereof. The deletion, mutation, and/or substitution in the endogenous gene encoding a polypeptide affecting Fe—S cluster biosynthesis can be selected from the group consisting of FRA2, GRX3, MSN5, and combinations thereof. 
     The present invention also provides a method for increasing the activity of an Fe—S cluster requiring protein in a recombinant host cell comprising providing a recombinant host cell comprising an Fe—S cluster requiring protein, changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis in the host cell, and growing the recombinant host cell with the changed expression or activity under conditions whereby the activity of the Fe—S cluster requiring protein is increased. Such a method can be used to increase the activity of an endogenous Fe—S cluster requiring protein, or a heterologous Fe—S cluster requiring protein. Such a method can be used to increase the specific activity of a DHAD described herein, or identified by the methods described herein. The increase in the activity of the Fe—S cluster requiring protein can be in an amount selected from greater than about 10%; greater than about 15%; greater than about 20%; greater than about 25%; greater than about 30%; greater than about 35%; greater than about 40%; greater than about 45%; greater than about 50%; greater than about 55%; greater than about 60%; greater than about 65%; greater than about 70%; greater than about 75%; greater than about 80%; greater than about 85%; greater than about 90%; and greater than about 95%. The increase in activity may be greater than about 3 fold, greater than about 5 fold, greater than about 8 fold, or greater than about 10 fold. In embodiments, the activity of the Fe—S cluster requiring protein can be in an amount that is at least about 60% of theoretical, at least about 70% of theoretical, at least about 80% theoretical, or at least about 90% theoretical. 
     The present invention can also be used to increase the flux in the Fe—S cluster biosynthesis pathway in a host cell and to identify polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell. In one embodiment a method is provided for increasing the flux in an Fe—S cluster biosynthesis pathway in a host cell comprising providing a recombinant host cell comprising an Fe—S cluster requiring protein and either at least one polypeptide affecting Fe—S cluster biosynthesis, at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting Fe—S cluster biosynthesis, or a combination of both, and growing the recombinant host cell under conditions whereby the flux in the Fe—S cluster biosynthesis pathway in the host cell is increased. In another embodiment, a method is provided for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell comprising: (a) changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis; (b) measuring the activity of a Fe—S cluster requiring protein; and (c) comparing the activity of the Fe—S cluster requiring protein measured in the presence of the change in expression or activity polypeptide of step (a) to the activity of the Fe—S cluster requiring protein measured in the absence of the change in expression or activity polypeptide of step (a), wherein an increase in the activity of the heterologous Fe—S cluster requiring protein indicates an increase in the flux in said Fe—S cluster biosynthesis pathway. In such methods, the Fe—S cluster requiring protein may be endogenous or heterologous to the host cell. 
     The expression or activity of the polypeptide affecting Fe—S cluster biosynthesis can be changed by methods well known in the art, including, but not limited to, deleting, mutating, substituting, expressing, up-regulating, down-regulating, altering the cellular location, altering the state of the protein, and/or adding a cofactor, and combinations thereof. Altering the state of the protein can include, but are not limited to, such alterations as phosphorylation or ubiquitination. Any number of methods described herein or known in the art can be used to measure the activity of the Fe—S cluster requiring protein, depending upon the Fe—S cluster requiring protein chosen. For example, if DHAD is the Fe—S cluster requiring protein, the assay described in the Example 7 can be used to measure the activity of the DHAD to determine if there is an increase in the flux in the Fe—S cluster biosynthesis pathway of the host cell. 
     Isobutanol and Other Products 
     Expression of a DHAD in a recombinant host cell, as described herein, provides the transformed, recombinant host cell with dihydroxy-acid dehydratase activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. A product that has α-ketoisovalerate or α-ketomethylvalerate as a pathway intermediate may be produced with greater effectiveness in a host cell disclosed herein having the described heterologous DHAD. A list of such products includes, but is not limited to, valine, isoleucine, leucine, pantothenic acid, 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol. 
     For example, biosynthesis of valine in yeast includes steps of acetolactate conversion to 2,3-dihydroxy-isovalerate by acetohydroxyacid reductoisomerase (ILV5), conversion of 2,3-dihydroxy-isovalerate to α-ketoisovalerate (also called 2-ketoisovalerate) by dihydroxy-acid dehydratase, and conversion of α-ketoisovalerate to valine by branched-chain amino acid transaminase (BAT2) and branched-chain amino acid aminotransferase (BAT1). Biosynthesis of leucine includes the same steps to α-ketoisovalerate, followed by conversion of α-ketoisovalerate to alpha-isopropylmalate by alpha-isopropylmalate synthase (LEU9, LEU4), conversion of alpha-isopropylmalate to beta-isopropylmalate by isopropylmalate isomerase (LEU1), conversion of beta-isopropylmalate to alpha-ketoisocaproate by beta-IPM dehydrogenase (LEU2), and finally conversion of alpha-ketoisocaproate to leucine by branched-chain amino acid transaminase (BAT2) and branched-chain amino acid aminotransferase (BAT1). The bacterial pathway is similar, involving differently named proteins and genes. Increased conversion of 2,3-dihydroxy-isovalerate to α-ketoisovalerate will increase flow in these pathways, particularly if one or more additional enzymes of a pathway is overexpressed. Thus, it is desired for production of valine or leucine to use a strain disclosed herein. 
     Biosynthesis of pantothenic acid includes a step performed by DHAD, as well as steps performed by ketopantoate hydroxymethyltransferase and pantothenate synthase. Engineering of expression of these enzymes for enhanced production of pantothenic acid biosynthesis in microorganisms is described in U.S. Pat. No. 6,177,264. 
     The α-ketoisovalerate product of DHAD is an intermediate in isobutanol biosynthetic pathways disclosed in U.S. Patent Appl. Pub. No. 20070092957 A1, which is incorporated by reference herein. A diagram of disclosed isobutanol biosynthetic pathways is provided in  FIG. 5 . Production of isobutanol in a strain disclosed herein may benefit from increased DHAD activity. As disclosed herein, increased DHAD activity is provided by expression of a DHAD in a host cell, for example, by over-expressing the DHAD, by modulating the expression or activity of a polypeptide having Fe—S cluster regulatory activity, or a combination of both expression of a DHAD and modulation of the expression or activity of a polypeptide having Fe—S cluster regulatory activity. As described in U.S. Patent Appl. Pub. No. 20070092957 A1, which is incorporated by reference herein, steps in an example isobutanol biosynthetic pathway include conversion of:
         pyruvate to acetolactate (see  FIG. 5 , pathway step a therein), as catalyzed for example by acetolactate synthase,   acetolactate to 2,3-dihydroxyisovalerate (see  FIG. 5 , pathway step b therein) as catalyzed for example by acetohydroxy acid isomeroreductase;   2,3-dihydroxyisovalerate to α-ketoisovalerate (see  FIG. 5 , pathway step c therein) as catalyzed for example by acetohydroxy acid dehydratase, also called dihydroxy-acid dehydratase (DHAD);   α-ketoisovalerate to isobutyraldehyde (see  FIG. 5 , pathway step d therein) as catalyzed for example by branched-chain α-keto acid decarboxylase; and   isobutyraldehyde to isobutanol (see  FIG. 5 , pathway step e therein) as catalyzed for example by branched-chain alcohol dehydrogenase.       

     The substrate to product conversions, and enzymes involved in these reactions, for steps f, g, h, I, j, and k of alternative pathways are described in U.S. Patent Appl. Pub. No. 20070092957 A1, which is incorporated by reference herein. 
     Genes that can be used for expression of the pathway step enzymes named above other than the DHADs disclosed herein, as well as those for additional isobutanol pathways, are described in U.S. Patent Appl. Pub. No. 20070092957 A1, which is incorporated by reference herein. Additional genes that may be used can be identified by one skilled in the art through bioinformatics or using methods well-known in the art, such as the various methods described in U.S. application Ser. No. 12/569,636, filed Sep. 29, 2009, which is incorporated by reference herein, to isolate homologs. Suitable ketol-acid reductoisomerase (KARI) enzymes are described in U.S. Patent Appl. Pub. Nos. 20080261230 A1, 20090163376, 20100197519, and U.S. application Ser. No. 12/893,077, all incorporated by reference herein. Examples of KARIs disclosed therein are those from  Vibrio cholerae, Pseudomonas aeruginosa  PAO1, and  Pseudomonas fluorescens  PF5. U.S. Patent Appl. Publ. No. 2009/0269823 and U.S. Prov. Patent Appl. No. 61/290,636, incorporated by reference herein, describe suitable alcohol dehydrogenases. 
     Additionally described in U.S. Patent Appl. Pub. No. 20070092957 A1, which is incorporated by reference herein, are construction of chimeric genes and genetic engineering of bacteria and yeast for isobutanol production using the disclosed biosynthetic pathways. 
     Additional Modifications 
     Examples of additional modifications that may be useful in cells provided herein include modifications to reduce glycerol-3-phosphate dehydrogenase activity and/or disruption in at least one gene encoding a polypeptide having pyruvate decarboxylase activity or a disruption in at least one gene encoding a regulatory element controlling pyruvate decarboxylase gene expression as described in U.S. Patent Appl. Pub. No. 20090305363 (incorporated herein by reference), modifications to a host cell that provide for increased carbon flux through an Entner-Doudoroff Pathway or reducing equivalents balance as described in U.S. Patent Appl. Pub. No. 20100120105 (incorporated herein by reference). Other modifications include integration of at least one polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway described in U.S. Prov. Appl. No. 61/380,563 (incorporated herein by reference). Additional modifications that may be suitable are described in U.S. application. Ser. No. 12/893,089. Additionally, host cells comprising a heterologous polynucleotide encoding a polypeptide with phosphoketolase activity and host cells comprising a heterologous polynucleotide encoding a polypeptide with phosphotransacetylase activity are described in U.S. Provisional Patent Application No. 61/356,379. 
     Growth for Production 
     Recombinant host cells disclosed herein are grown in fermentation media which contains suitable carbon substrates. Suitable carbon substrates may include, but are not limited to, monosaccharides such as glucose, fructose, oligosaccharides such as lactose maltose, galactose, or sucrose, polysaccharides such as starch or cellulose or mixtures thereof and unpurified mixtures from renewable feedstocks such as cheese whey permeate, cornsteep liquor, sugar beet molasses, and barley malt. Other carbon substrates may include ethanol, lactate, succinate, or glycerol. 
     Additionally the carbon substrate may also be one-carbon substrates such as carbon dioxide, or methanol for which metabolic conversion into key biochemical intermediates has been demonstrated. Two-carbon substrates such as ethanol may also suitable. In addition to one and two carbon substrates, methylotrophic organisms are also known to utilize a number of other carbon containing compounds such as methylamine, glucosamine and a variety of amino acids for metabolic activity. For example, methylotrophic yeasts are known to utilize the carbon from methylamine to form trehalose or glycerol (Bellion et al.,  Microb. Growth C 1  Compd ., [Int. Symp.], 7th (1993), 415-32, Editor(s): Murrell, J. Collin; Kelly, Don P. Publisher: Intercept, Andover, UK). Similarly, various species of  Candida  will metabolize alanine or oleic acid (Sulter et al.,  Arch. Microbiol.  153:485-489 (1990)). Hence it is contemplated that the source of carbon utilized in the present invention may encompass a wide variety of carbon containing substrates and will only be limited by the choice of organism. 
     Although it is contemplated that all of the above mentioned carbon substrates and mixtures thereof are suitable in the present invention, in some embodiments, the carbon substrates are glucose, fructose, and sucrose, or mixtures of these with C5 sugars such as xylose and/or arabinose for yeasts cells modified to use C5 sugars. Sucrose may be derived from renewable sugar sources such as sugar cane, sugar beets, cassava, sweet sorghum, and mixtures thereof. Glucose and dextrose may be derived from renewable grain sources through saccharification of starch based feedstocks including grains such as corn, wheat, rye, barley, oats, and mixtures thereof. In addition, fermentable sugars may be derived from renewable cellulosic or lignocellulosic biomass through processes of pretreatment and saccharification, as described, for example, in co-owned and co-pending U.S. Patent Appl. Pub. No. 20070031918 A1, which is herein incorporated by reference. Biomass refers to any cellulosic or lignocellulosic material and includes materials comprising cellulose, and optionally further comprising hemicellulose, lignin, starch, oligosaccharides and/or monosaccharides. Biomass may also comprise additional components, such as protein and/or lipid. Biomass may be derived from a single source, or biomass can comprise a mixture derived from more than one source; for example, biomass may comprise a mixture of corn cobs and corn stover, or a mixture of grass and leaves. Biomass includes, but is not limited to, bioenergy crops, agricultural residues, municipal solid waste, industrial solid waste, sludge from paper manufacture, yard waste, wood and forestry waste. Examples of biomass include, but are not limited to, corn grain, corn cobs, crop residues such as corn husks, corn stover, grasses, wheat, wheat straw, barley, barley straw, hay, rice straw, switchgrass, waste paper, sugar cane bagasse, sorghum, soy, components obtained from milling of grains, trees, branches, roots, leaves, wood chips, sawdust, shrubs and bushes, vegetables, fruits, flowers, animal manure, and mixtures thereof. 
     In addition to an appropriate carbon source, growth media must contain suitable minerals, salts, cofactors, buffers and other components, known to those skilled in the art, suitable for the growth of the cultures and promotion of an enzymatic pathway comprising a Fe—S cluster requiring protein such as, for example, DHAD. 
     Culture Conditions 
     Typically cells are grown at a temperature in the range of about 20° C. to about 40° C. in an appropriate medium. Suitable growth media in the present invention are common commercially prepared media such as Luria Bertani (LB) broth, Sabouraud Dextrose (SD) broth, Yeast Medium (YM) broth, or broth that includes yeast nitrogen base, ammonium sulfate, and dextrose (as the carbon/energy source) or YPD Medium, a blend of peptone, yeast extract, and dextrose in optimal proportions for growing most  Saccharomyces cerevisiae  strains. Other defined or synthetic growth media may also be used, and the appropriate medium for growth of the particular microorganism will be known by one skilled in the art of microbiology or fermentation science. The use of agents known to modulate catabolite repression directly or indirectly, e.g., cyclic adenosine 2′:3′-monophosphate, may also be incorporated into the growth medium. 
     Suitable pH ranges for the growth are between about pH 5.0 to about pH 9.0. In one embodiment, about pH 6.0 to about pH 8.0 is used for the initial condition. Suitable pH ranges for the fermentation of yeast are typically between about pH 3.0 to about pH 9.0. In one embodiment, about pH 5.0 to about pH 8.0 is used for the initial condition. Suitable pH ranges for the fermentation of other microorganisms are between about pH 3.0 to about pH 7.5. In one embodiment, about pH 4.5 to about pH 6.5 is used for the initial condition. 
     Growth may be performed under aerobic or anaerobic conditions. In one embodiment, anaerobic or microaerobic conditions are used for growth. 
     Industrial Batch and Continuous Fermentations 
     Isobutanol, or other products, may be produced using a batch method of fermentation. A classical batch fermentation is a closed system where the composition of the medium is set at the beginning of the fermentation and not subject to artificial alterations during the fermentation. A variation on the standard batch system is the fed-batch system. Fed-batch fermentation processes are also suitable in the present invention and comprise a typical batch system with the exception that the substrate is added in increments as the fermentation progresses. Fed-batch systems are useful when catabolite repression is apt to inhibit the metabolism of the cells and where it is desirable to have limited amounts of substrate in the media. Batch and fed-batch fermentations are common and well known in the art and examples may be found in Thomas D. Brock in  Biotechnology: A Textbook of Industrial Microbiology , Second Edition (1989) Sinauer Associates, Inc., Sunderland, Mass., or Deshpande, Mukund V.,  Appl. Biochem. Biotechnol.,  36:227, (1992), herein incorporated by reference. 
     Isobutanol, or other products, may also be produced using continuous fermentation methods. Continuous fermentation is an open system where a defined fermentation medium is added continuously to a bioreactor and an equal amount of conditioned media is removed simultaneously for processing. Continuous fermentation generally maintains the cultures at a constant high density where cells are primarily in log phase growth. Continuous fermentation allows for the modulation of one factor or any number of factors that affect cell growth or end product concentration. Methods of modulating nutrients and growth factors for continuous fermentation processes as well as techniques for maximizing the rate of product formation are well known in the art of industrial microbiology and a variety of methods are detailed by Brock, supra. 
     It is contemplated that the production of isobutanol, or other products, may be practiced using batch, fed-batch or continuous processes and that any known mode of fermentation would be suitable. Additionally, it is contemplated that cells may be immobilized on a substrate as whole cell catalysts and subjected to fermentation conditions for isobutanol production. 
     Methods for Isobutanol Isolation from the Fermentation Medium 
     Bioproduced isobutanol may be isolated from the fermentation medium using methods known in the art for ABE fermentations (see, e.g., Durre,  Appl. Microbiol. Biotechnol.  49:639-648 (1998), Groot et al.,  Process. Biochem.  27:61-75 (1992), and references therein). For example, solids may be removed from the fermentation medium by centrifugation, filtration, decantation, or the like. Then, the isobutanol may be isolated from the fermentation medium using methods such as distillation, azeotropic distillation, liquid-liquid extraction, adsorption, gas stripping, membrane evaporation, or pervaporation. 
     Because isobutanol forms a low boiling point, azeotropic mixture with water, distillation can be used to separate the mixture up to its azeotropic composition. Distillation may be used in combination with another separation method to obtain separation around the azeotrope. Methods that may be used in combination with distillation to isolate and purify butanol include, but are not limited to, decantation, liquid-liquid extraction, adsorption, and membrane-based techniques. Additionally, butanol may be isolated using azeotropic distillation using an entrainer (see, e.g., Doherty and Malone,  Conceptual Design of Distillation Systems , McGraw Hill, New York, 2001). 
     The butanol-water mixture forms a heterogeneous azeotrope so that distillation may be used in combination with decantation to isolate and purify the isobutanol. In this method, the isobutanol containing fermentation broth is distilled to near the azeotropic composition. Then, the azeotropic mixture is condensed, and the isobutanol is separated from the fermentation medium by decantation. The decanted aqueous phase may be returned to the first distillation column as reflux. The isobutanol-rich decanted organic phase may be further purified by distillation in a second distillation column. 
     The isobutanol may also be isolated from the fermentation medium using liquid-liquid extraction in combination with distillation. In this method, the isobutanol is extracted from the fermentation broth using liquid-liquid extraction with a suitable solvent. The isobutanol-containing organic phase is then distilled to separate the butanol from the solvent. 
     Distillation in combination with adsorption may also be used to isolate isobutanol from the fermentation medium. In this method, the fermentation broth containing the isobutanol is distilled to near the azeotropic composition and then the remaining water is removed by use of an adsorbent, such as molecular sieves (Aden et al.  Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co - Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover , Report NREL/TP-510-32438, National Renewable Energy Laboratory, June 2002). 
     Additionally, distillation in combination with pervaporation may be used to isolate and purify the isobutanol from the fermentation medium. In this method, the fermentation broth containing the isobutanol is distilled to near the azeotropic composition, and then the remaining water is removed by pervaporation through a hydrophilic membrane (Guo et al.,  J. Membr. Sci.  245, 199-210 (2004)). 
     Embodiments of the Inventions 
     Embodiment 1 (E1). A recombinant host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity wherein said at least one heterologous polynucleotide comprises a high copy number plasmid or a plasmid with a copy number that can be regulated. 
     E2. A recombinant host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity wherein said at least one heterologous polynucleotide is integrated at least once in the recombinant host cell DNA. 
     E3. A recombinant host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity, wherein said host cell comprises at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting Fe—S cluster biosynthesis. 
     E4. A recombinant host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity and at least one heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis. 
     E5. The recombinant host cell of any one of embodiments E3-E4, wherein said heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of the genes in Tables 8 and 9. 
     E6. The recombinant host cell of any one of embodiments E3-E4, wherein said heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of the genes in Table 7. 
     E7. The recombinant host cell of embodiment E5 or E6, wherein said heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of AFT1, AFT2, PSE1, FRA2, GRX3, MSN5, and combinations thereof. 
     E8. The recombinant host cell of embodiment E7, wherein said polypeptide is encoded by a polynucleotide that is constitutive mutant. 
     E9. The recombinant host cell of embodiment E8, wherein said constitutive mutant is selected from the group consisting of AFT1 L99A, AFT1 L102A, AFT1 C291F, AFT1 C293F, and combinations thereof. 
     E10. The recombinant host cell of embodiment E7, wherein said polypeptide affecting Fe—S cluster biosynthesis is encoded by a polynucleotide comprising a high copy number plasmid or a plasmid with a copy number that can be regulated. 
     E11. The recombinant host cell of embodiment E7, wherein said polypeptide affecting Fe—S cluster biosynthesis is encoded by a polynucleotide integrated at least once in the recombinant host cell DNA. 
     E12. The recombinant host cell of embodiment E3, wherein the at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of FRA2, GRX3, MSN5, and combinations thereof. 
     E13. The recombinant host cell of embodiment E4, wherein the at least one heterologous polynucleotide encoding a polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of AFT1, AFT2, PSE1, and combinations thereof. 
     E14. The recombinant host cell of any one of embodiments E3-E13, wherein said at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity is expressed in multiple copies. 
     E15. The recombinant host cell of embodiment E14, wherein said at least one heterologous polynucleotide comprises a high copy number plasmid or a plasmid with a copy number that can be regulated. 
     E16. The recombinant host cell of embodiment E14, wherein said at least one heterologous polynucleotide is integrated at least once in the recombinant host cell DNA. 
     E17. The recombinant host cell of any one of embodiments E3-E16, wherein said Fe—S cluster biosynthesis is increased compared to a recombinant host cell having endogenous Fe—S cluster biosynthesis. 
     E18. The recombinant host cell of any one of embodiments E1-E17, wherein said host cell is a yeast host cell. 
     E19. The recombinant host cell of embodiment E18, wherein said yeast host cell is selected from the group consisting of  Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia  and  Pichia.    
     E20. The recombinant host cell of any one of embodiments E1-E19, wherein said heterologous polypeptide having dihydroxy-acid dehydratase activity is expressed in the cytosol of the host cell. 
     E21. The recombinant host cell of any one of embodiments E1-E20, wherein said heterologous polypeptide having dihydroxy-acid dehydratase activity has an amino acid sequence that matches the Profile HMM of Table 12 with an E value of &lt;10 −5  wherein the polypeptide further comprises all three conserved cysteines, corresponding to positions 56, 129, and 201 in the amino acids sequences of the  Streptococcus mutans  DHAD enzyme corresponding to SEQ ID NO:168. 
     E22. The recombinant host cell of any one of embodiments E1-E21, wherein said heterologous polypeptide having dihydroxy-acid dehydratase activity has an amino acid sequence with at least about 90% identity to SEQ ID NO: 168 or SEQ ID NO: 232. 
     E23. The recombinant host cell of any one of embodiments E1-E22, wherein said polypeptide having dihydroxy-acid dehydratase activity has a specific activity selected from the group consisting of:
         a. greater than about 5-fold with respect to the control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity;   b. greater than about 8-fold with respect to the control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity; and   c. greater than about 10-fold with respect to the control host cell comprising at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity.       

     E24. The recombinant host cell of any one of embodiments E1-E22, wherein said polypeptide having dihydroxy-acid dehydratase activity has a specific activity selected from the group consisting of:
         a. greater than about 0.25 U/mg;   b. greater than about 0.3 U/mg;   c. greater than about 0.5 U/mg;   d. greater than about 1.0 U/mg;   e. greater than about 1.5 U/mg;   f. greater than about 2.0 U/mg;   g. greater than about 3.0 U/mg;   h. greater than about 4.0 U/mg;   i. greater than about 5.0 U/mg;   j. greater than about 6.0 U/mg;   k. greater than about 7.0 U/mg;   l. greater than about 8.0 U/mg;   m. greater than about 9.0 U/mg;   n. greater than about 10.0 U/mg;   o. greater than about 20.0 U/mg; and   p. greater than about 50.0 U/mg.       

     E25. The recombinant host cell of any one of embodiments E1-E24, wherein said recombinant host cell produces isobutanol. 
     E26. The recombinant host cell of embodiment E25, wherein said recombinant host cell comprises an isobutanol biosynthetic pathway. 
     E27. A method of making a product comprising:
         a. providing the recombinant host cell of any one of embodiments E1-E24; and   b. contacting the recombinant host cell of (a) with a fermentable carbon substrate in a fermentation medium under conditions wherein said product is produced; wherein the product is selected from the group consisting of branched chain amino acids, pantothenic acid, 2-methyl-1-butanol, 3-methyl-1-butanol, isobutanol, and combinations thereof.       

     E28. A method of making isobutanol comprising:
         a. providing the recombinant host cell of any one of embodiments E1-E24;   b. contacting the recombinant host cell of (a) with a fermentable carbon substrate in a fermentation medium under conditions wherein isobutanol is produced.       

     E29. A method for the conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate comprising:
         a. providing the recombinant host of any one of embodiments E1-E24;   b. growing the recombinant host cell of (a) under conditions where the 2,3-dihydroxyisovalerate is converted to α-ketoisovalerate, wherein 2,3-dihydroxyisovalerate is converted to α-ketoisovalerate.       

     E30. A method for increasing the specific activity of a heterologous polypeptide having dihydroxy-acid dehydratase activity in a recombinant host cell comprising:
         a. providing a recombinant host cell of any one of embodiments E1-E24; and   b. growing the recombinant host cell of (a) under conditions whereby the heterologous polypeptide having dihydroxy-acid dehydratase activity is expressed in functional form having a specific activity greater than the same host cell lacking said heterologous polypeptide.       

     E31. A method for increasing the flux in an Fe—S cluster biosynthesis pathway in a host cell comprising:
         a. providing a recombinant host cell of any one of embodiments E3-E24; and   b. growing the recombinant host cell of (a) under conditions whereby the flux in the Fe—S cluster biosynthesis pathway in the host cell is increased.       

     E32. A method of increasing the activity of an Fe—S cluster requiring protein in a recombinant host cell comprising:
         a. providing a recombinant host cell comprising an Fe—S cluster requiring protein;   b. changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis in said host cell; and   c. growing the recombinant host cell of (b) under conditions whereby the activity of the Fe—S cluster requiring protein is increased.       

     E33. The method of embodiment E32, wherein said increase in activity is an amount selected from the group consisting of:
         a. greater than about 10%;   b. greater than about 20%;   c. greater than about 30%;   d. greater than about 40%;   e. greater than about 50%;   f. greater than about 60%;   g. greater than about 70%;   h. greater than about 80%;   i. greater than about 90%; and   j. greater than about 95%.       

     E34. The method of embodiment E32, wherein said increase in activity is an amount selected from the group consisting of:
         a. greater than about 5 fold;   b. greater than about 8 fold;   c. greater than about 10 fold.       

     E35. A method for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell comprising:
         a. changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis;   b. measuring the activity of a heterologous Fe—S cluster requiring protein; and   c. comparing the activity of the heterologous Fe—S cluster requiring protein measured in the presence of the changed expression or activity of a polypeptide of step (a) to the activity of the heterologous Fe—S cluster requiring protein measured in the absence of the changed expression or activity of a polypeptide of step (a),   wherein an increase in the activity of the heterologous Fe—S cluster requiring protein indicates an increase in the flux in said Fe—S cluster biosynthesis pathway.       

     E36. A method for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell comprising:
         a. changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis;   b. measuring the activity of a polypeptide having dihydroxy-acid dehydratase activity; and   c. comparing the activity of the polypeptide having dihydroxy-acid dehydratase activity measured in the presence of the change in expression or activity of a polypeptide of step (a) to the activity of the polypeptide having dihydroxy-acid dehydratase activity measured in the absence of the change in expression or activity of a polypeptide of step (a),   wherein an increase in the activity of the polypeptide having dihydroxy-acid dehydratase activity indicates an increase in the flux in said Fe—S cluster biosynthesis pathway.       

     E37. The method of any one of embodiments E30-E36, wherein said changing the expression or activity of a polypeptide affecting Fe—S cluster biosynthesis comprises deleting, mutating, substituting, expressing, up-regulating, down-regulating, altering the cellular location, altering the state of the protein, and/or adding a cofactor. 
     E38. The method of any one of embodiments E32-E37, wherein the Fe—S cluster requiring protein has dihydroxy-acid dehydratase activity and wherein said Fe—S cluster requiring protein having dihydroxy-acid dehydratase activity has an amino acid sequence that matches the Profile HMM of Table 12 with an E value of &lt;10 −5  wherein the polypeptide further comprises all three conserved cysteines, corresponding to positions 56, 129, and 201 in the amino acids sequences of the  Streptococcus mutans  DHAD enzyme corresponding to SEQ ID NO:168. 
     E39. The method of any one of embodiments E32-E38, wherein said polypeptide affecting Fe—S cluster biosynthesis is selected from the group consisting of the genes in Tables 7, 8 and 9. 
     E40. A recombinant host cell comprising at least one polynucleotide encoding a polypeptide identified by the methods of any one of embodiments E35-E37. 
     E41. The recombinant host cell of embodiment E40, wherein said host cell further comprises at least one heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity. 
     E42. The recombinant host cell of embodiment E41, wherein said heterologous polynucleotide encoding a polypeptide having dihydroxy-acid dehydratase activity is expressed in multiple copies. 
     E43. The recombinant host cell of embodiment E41, wherein said heterologous polynucleotide comprises a high copy number plasmid or a plasmid with a copy number that can be regulated. 
     E44. The recombinant host cell of embodiment E41, wherein said heterologous polynucleotide is integrated at least once in the recombinant host cell DNA. 
     E45. The method of embodiment E35 or E36, wherein said host cell is a yeast host cell. 
     E46. The method of embodiment E45, wherein said yeast host cell is selected from the group consisting of  Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia , and  Pichia.    
     E47. The method of any one of embodiments E28-E39, wherein said host cell is a yeast host cell. 
     E48. The method of embodiment E47, wherein said yeast host cell is selected from the group consisting of  Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia , and  Pichia.    
     E49. The recombinant host cell of any one of embodiments E40-E44, wherein said recombinant host cell is a yeast host cell. 
     E50. The recombinant host cell of embodiment E49, wherein said yeast host cell is selected from the group consisting of  Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia , and  Pichia.    
     E51. The recombinant host cell of any one of embodiments E40-E44 or E49-E50, wherein said heterologous polypeptide having dihydroxy-acid dehydratase activity is expressed in the cytosol of the host cell. 
     E52. The recombinant host cell of any one of embodiments E40-E44 or E49-E50, wherein said heterologous polypeptide having dihydroxy-acid dehydratase activity has an amino acid sequence that matches the Profile HMM of Table 12 with an E value of &lt;10 −5  wherein the polypeptide further comprises all three conserved cysteines, corresponding to positions 56, 129, and 201 in the amino acids sequences of the  Streptococcus mutans  DHAD enzyme corresponding to SEQ ID NO:168. 
     E53. The recombinant host cell of any one of embodiments E40-E44 or E49-E50, wherein said recombinant host cell produces a product selected from the group consisting of branched chain amino acids, pantothenic acid, 2-methyl-1-butanol, 3-methyl-1-butanol, isobutanol, and combinations thereof. 
     E54. The recombinant host cell of embodiment E53, wherein said recombinant host cell produces isobutanol. 
     E55. The recombinant host cell of embodiment E54, wherein said recombinant host cell comprises an isobutanol biosynthetic pathway. 
     EXAMPLES 
     The meaning of abbreviations used is as follows: “min” means minute(s), “h” means hour(s), “sec” means second(s), “μl” means microliter(s), “ml” means milliliter(s), “L” means liter(s), “nm” means nanometer(s), “mm” means millimeter(s), “cm” means centimeter(s), “μm” means micrometer(s), “mM” means millimolar, “M” means molar, “mmol” means millimole(s), “μmole” means micromole(s), “g” means gram(s), “μg” means microgram(s), “mg” means milligram(s), “rpm” means revolutions per minute, “w/v” means weight/volume, “OD” means optical density, and “OD 600 ” means optical density measured at a wavelength of 600 nm. 
     General Methods: 
     Standard recombinant DNA and molecular cloning techniques used in the Examples are well known in the art and are described by Sambrook, J., Fritsch, E. F. and Maniatis, T.,  Molecular Cloning: A Laboratory Manual , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989, by T. J. Silhavy, M. L. Bennan, and L. W. Enquist,  Experiments with Gene Fusions , Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1984, and by Ausubel, F. M. et al.,  Current Protocols in Molecular Biology,  Greene Publishing Assoc. and Wiley-Interscience, N.Y., 1987. 
     Materials and methods suitable for the maintenance and growth of bacterial cultures are also well known in the art. Techniques suitable for use in the following Examples may be found in  Manual of Methods for General Bacteriology , Phillipp Gerhardt, R. G. E. Murray, Ralph N. Costilow, Eugene W. Nester, Willis A. Wood, Noel R. Krieg and G. Briggs Phillips, eds., American Society for Microbiology, Washington, D.C., 1994, or by Thomas D. Brock in  Biotechnology: A Textbook of Industrial Microbiology , Second Edition, Sinauer Associates, Inc., Sunderland, Mass., 1989. All reagents, restriction enzymes and materials used for the growth and maintenance of bacterial cells were obtained from Aldrich Chemicals (Milwaukee, Wis.), BD Diagnostic Systems (Sparks, Md.), Life Technologies (Rockville, Md.), or Sigma Chemical Company (St. Louis, Mo.), unless otherwise specified. 
     Example 1 
     Over-expression of DHAD Protein Encoded by the ilvD Gene from  S. mutans  using a Plasmid-based System in Yeast Cytosol 
     Over-expression of a recombinant polynucleotide can be accomplished by increasing the copy number of a plasmid comprising the recombinant polynucleotide. To over-express the DHAD protein in yeast, an inducible vector was constructed. The pHR81 vector contains a Ura3 marker as well as a LEU marker with a defective promoter (see U.S. Patent Appl. Pub. No. 2007/0092957, which is incorporated by reference herein). When the yeast synthetic dropout (SD; also known as complete minimal media; Teknova) growth medium is switched from SD minus uracil to SD minus leucine, the copy number of the pHR81 plasmid increases, resulting in much higher level of expression of the recombinant polynucleotide. The pHR81 vector backbone was derived from pLH472 JEG4y (SEQ ID NO: 921) and was prepared by digesting the pLH472 JEG4y vector with SpeI and SacII. 
     For over-expression of a DHAD protein, the DHAD gene ilvD from  S. mutans  (SEQ ID NO:167) was used (see U.S. Published Patent Appl. No. US2009-0305363A1, which is incorporated by reference herein). This gene has been cloned under the control of the FBA promoter in vector pRS423 FBA ilvD Strep-lumio (see U.S. Published Patent Appl. No. US2009-0305363A1, which is incorporated by reference herein). The region containing the FBA promoter, the ilvD gene, and FBA terminator cassette was amplified with primer set FBAp-F(NheI) and FBAt-R(SacII) (SEQ ID NOs: 915 and 916) and cloned into the pHR81 vector. The resulting expression vector was designated as pHR81 FBA-IlvD(Sm) (SEQ ID NO: 917;  FIG. 1A ). 
     To over express the  S. mutans  DHAD protein, the expression vector pHR81 FBA-IlvD(Sm) was transformed into wild-type yeast strain BY4741. Transformants were selected on agar plates with SD minus uracil. For over-expression, yeast strains containing the plasmid were initially grown at 30° C. in SD liquid medium minus uracil. A fresh overnight culture (5 ml) was then transferred to a 125 ml flask containing 75 ml of SD medium minus leucine. As a control, another 5 ml of fresh overnight culture was transferred into a flask containing 75 ml of SD minus uracil. The cultures were incubated overnight before harvesting by centrifugation. The DHAD activity was measured in crude extracts of these samples using the assay described in Example 7. 
     The DHAD specific activity obtained in the crude extract in the control samples grown in SD minus uracil was in the range of 0.2 to 0.3 U mg −1 . The average specific activity obtained from strains grown in the SD medium minus leucine, however, was 1.6 U mg −1 , much higher (˜5 to 8-fold higher) than the activity from the control samples. DHAD requires Fe—S cluster for its function, and it was not previously known if the native yeast Fe—S cluster biosynthesis pathway could accommodate an over-expressed Fe—S cluster requiring protein in yeast cytosol. In a previous screening experiment using a non-inducible, low-copy number vector, the DHAD from  S. mutans  could be recombinantly expressed in yeast cytosol with a specific activity in the range of 0.1 to 0.2 U mg −1  in the crude extract (see U.S. patent application Ser. No. 12/569,636, filed on Sep. 29, 2009, which is incorporated by reference herein). Thus, in one embodiment, over-expression of a Fe—S cluster requiring protein, such as DHAD, in yeast using a high-copy number vector provides increased specific activity, wherein the specific activity is increased by at least about 5-fold to at least about 8-fold. 
     Example 2 
     Over-Expression of DHAD Protein Encoded by the ilvD Gene from  S. mutans  Through Chromosomal Integration 
     An alternate way to increase the expression of a gene in yeast is to integrate multiple copies of the gene of interest into the host cell&#39;s chromosome. To integrate the ilvD gene from  S. mutans  (SEQ ID NO:167) into a yeast chromosome, integration vector pZK-Delta(s)-Leu2-FBA-ilvD(Sm)-FBAt (SEQ ID NO: 918;  FIG. 1B ) was constructed. The integration vector backbone was derived from pSuperscript (Stratagene, La Jolla, Calif.). The  S. mutans  ilvD gene (nucleotides 1306-3018 of the complement strand) was cloned into the integration vector under the control of the FBA promoter (nucleotides 3026-4023 of the complement strand) so that the ilvD gene would be flanked by a yeast delta sequence (nucleotides 118-267 and 5061-5760 of the complement strand).  S. cerevisiae  contains more than 200 yeast delta sequences (Kim J M et al. Genome Res. 1998; 8:464-478). These delta sequences are targets for multiple integrations. The integration vector was also engineered to contain the defective LEU2 marker (nucleotides 4100-5191 of the complement strand) for selection of transformed strains with multiple integration events. 
     For integration, the vector DNA was linearized with AscI and AatII digestion to generate delta sequence flanked strands of vector DNA comprising the ilvD gene, which were then transformed into the yeast strain BY4741. Transformants were selected on SD agar medium minus leucine. These transformants were then grown on SD liquid medium minus leucine at 30° C., and the cultures were harvested and analyzed for DHAD activity. The specific activity of DHAD obtained in the crude extract ranged from 0.7 to 1.2 U mg −1 . This specific activity was about 3- to 6-fold higher than that found in BY4741 strains transformed with an ilvD gene-containing plasmid without over-expression 
     Example 3 
     Improvement of Specific Activity of DHAD in Yeast Deletion Strains 
     Although the over-expression strains described in Examples 1 and 2 had a high level of activity, not all of the DHAD protein expressed was active. For example, the over-expressed DHAD protein accounted for approximately 5 to 10% of the total cell protein, while yielding a specific activity of from about 0.7 to 1.6 U mg −1 . Given that the specific activity of the purified DHAD enzyme from  S. mutans  is 100 U mg −1 , expression of DHAD at 10% of total cell protein would be expected to yield a specific activity upwards of 5 to 10 U mg −1 . Although not wishing to be bound by one theory, the difference between the expected and observed specific activity was likely a result of insufficient Fe—S cluster loading. Thus, increasing Fe—S cluster loading by further manipulating the over-expression strains could be used to increase the specific activity of DHAD. 
     In order to improve the specific activity, yeast strains with deletions in genes involved in iron metabolism and Fe—S cluster sensing were used to investigate their effects on DHAD specific activity. These strains (BY4741 background) were purchased from Open Biosystem (Huntsville, Ala.) and are listed in Table 10. As described in Example 1, the high copy number plasmid pHR81 FBA-IlvD(Sm) was transformed into these strains, and DHAD over-expression was induced by changing the growth medium to SD minus leucine. Crude extracts from cultures were prepared and assayed for DHAD activity. Results are shown in Table 10. 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Effects of deletions of genes involved in Fe metabolism. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Specific 
               
               
                 Genes 
                 Function 
                 Activity (U/mg) 
               
               
                   
               
               
                 WT 
                   
                 1.69 ± 0.02 
               
               
                 Δisu1 
                 scaffold protein for Fe—S cluster assembling 
                 1.31 ± 0.56 
               
               
                 Δfra2 
                 repressor component for Aft1p 
                 3.41 ± 0.24 
               
               
                 Δsin4 
                 regulatory protein 
                 1.65 ± 0.20 
               
               
                 Δmtm1 
                 protein involved in metal metabolism 
                 0.54 ± 0.12 
               
               
                 Δfra1 
                 regulatory protein 
                 0.97 ± 0.05 
               
               
                 Δgrx3 
                 glutaredoxins 
                 5.45 ± 0.14 
               
               
                 Δaft1 
                 global Fe regulator 
                 0.23 ± 0.05 
               
               
                 Δaft2 
                 paralogue to Aft1p 
                 1.11 ± 0.38 
               
               
                 Δmsn5 
                 nuclear protein exporter 
                 1.59 ± 0.10 
               
               
                 Δfet3 
                 ferrous iron uptake; multi-copper oxidase 
                 0.54 ± 0.09 
               
               
                 Δftr1 
                 ferrous iron uptake; permease 
                 0.76 ± 0.03 
               
               
                 Δccc2 
                 copper transporter (for Fet3p) 
                 1.23 ± 0.17 
               
               
                 Δgef1 
                 copper transporter/loading for Fet3p 
                 1.70 ± 0.10 
               
               
                 Δfet4 
                 Low-affinity Fe(II) transporter 
                 1.07 ± 0.02 
               
               
                 Δsmf1 
                 Low-affinity Fe(II) transporter 
                 1.78 ± 0.12 
               
               
                 Δmrs3 
                 mitochondrial iron transporter 
                 1.51 ± 0.13 
               
               
                 Δmrs4 
                 mitochondrial iron transporter 
                 0.85 ± 0.16 
               
               
                 Δcth2 
                 targeted mRNA binding and degradation 
                 1.28 ± 0.40 
               
               
                 Δcth1 
                 targeted mRNA binding and degradation 
                 1.44 ± 0.30 
               
               
                   
               
            
           
         
       
     
     Surprisingly, DHAD specific activity in the crude extract in strains with a deletion in either the FRA2 or the GRX3 gene increased by 2- to 3-fold, which was unexpected as many of the deletions tested did not increase DHAD specific activity. It has been shown that cytosolic iron sulfur assembly (CIA) machinery in yeast is responsible for assembly of Fe—S clusters for cytosolic proteins such as isopropylmalate isomerase (Leu1). Previous results indicate that this CIA machinery is independent from the iron sensing system involving Aft1 and a Grx3/Grx4-Fra2 heterodimer as the repressor (Rutherford et al,  J Biol Chem.  280:10135-10140 (2005)). 
     Another unexpected finding is the effect of a Grx3 deletion on DHAD activity. It has been shown that Grx3 and Grx4 are equivalent in function. While double mutations in both GRX3 and GRX4 genes resulted in drastic activation of the Fe regulon, mutation in Grx4 alone confers minimal phenotype (Pujol-Carrion, et al,  J Cell Sci.  119:4554-4564 (2006); Ojeda, et al,  J Biol Chem.  281:17661-17669 (2006).). As shown in Table 10 above, GRX3 deletion alone leads to significant improvement in DHAD specific activity. 
     Thus, these results demonstrate that modulating genes involved in iron metabolism can increase the activity of an Fe—S cluster requiring protein such as DHAD when expressed in yeast cytosol. As outlined in  FIG. 10 , the effect of deletions of the FRA2 and GRX3 genes on DHAD specific activity could result from, e.g., activation of transcription of one or more of the genes in the iron regulon via the global regulator Aft1p. Although not wishing to be bound by any one theory, activation of such genes could lead to an increase in iron uptake and an increase in cytoplasmic Fe—S cluster biosynthesis, leading to higher Fe—S cluster loading of the protein ( FIG. 10 ). Demonstration of increased Fe—S cluster loading is described in Example 11. 
     Example 4 
     Effect of Expression of Aft1p and its Mutants on DHAD Specific Activity 
     As described in Example 3 and outlined in  FIG. 10 , Fra2, Grx3, and Grx4 are repressors that regulate the function of Aft1p (Kumánovics, et al.,  J. Biol. Chem.  283:10276-10286 (2008)). Aft1p is a global regulator of iron. Activation of genes involved in iron uptake and metabolism requires the nuclear localization of Aft1p. Expression of Aft1 constitutive mutants or an increase in the expression of wild-type Aft1p, could lead to the activation of the Fe regulon in a wild-type strain or in an AFT1 deletion strain (Yamaguchi-Iwai, et al,  EMBO J.  14:1231-1239 (1995); Yamaguchi-Iwai, et al,  J. Biol. Chem.  277:18914-18918 (2002); Kaplan, et al,  Chem. Rev.  109:4536-4552(2009)). Based on the novel findings described in Example 3, it is possible that expression of Aft1p protein and its constitutive mutants may improve the active fraction of the DHAD enzyme which requires Fe—S clusters for its activity. 
     To examine this possibility, the wild-type AFT1 gene and its constitutive mutants were cloned using a centromere vector pRS411 (ATCC® Number: 87538; SEQ ID NO: 919). This vector has an ampicillin selection marker for growth in  E. coli  and a methionine nutritional marker for selection in yeast. The wild-type AFT1 gene, including its own promoter and terminator, can be cloned between the KpnI and SacI sites, resulting in the construct pRS411-Aft1+flanking (SEQ ID NO: 920;  FIG. 2 ). A similar strategy can be used to clone genes that encode Aft1 constitutive mutants. The Aft1 constitutive mutants with substitutions at amino acids L99 to A and C291 to F (with respect to SEQ ID NO: 703) were first examined. The pRS411 constructs with genes encoding the wild-type AFT1 gene or constitutive mutants were transformed, along with the expression vector pHR81 FBA IlvD(Sm), into the wild-type yeast strain BY4741 or a yeast strain with a deletion in AFT1, GRX3, or FRA2. Transformants were selected on agar plates with SD medium minus methionine and uracil. Transformed strains were grown in SD medium minus methionine and leucine to over-express the DHAD protein in the presence of these genes or mutants. The DHAD activity in the crude extract of these cultures were measured. 
     Results of expression of wild-type Aft1p, Aft1p(C291F), and Aft1p(L99A) are shown in Table 11. A moderate increase in DHAD specific activity was observed with Aft1p (C291F) as compared to wild-type Aft1p. A much higher increase in DHAD activity was observed with Aft1p(L99A). The specific activity of DHAD in yeast expressing Aft1p(L99A) was similar to the specific activity obtained in the GRX3 deletion strain (see Table 10). 
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Effects of expression of Aft1p and its mutants on the 
               
               
                 activity of DHAD from  S. mutans  in Δaft1 strain. 
               
            
           
           
               
               
            
               
                 Plasmids 
                 Specific Activity (U/mg) 
               
               
                   
               
               
                 pHR81-FBA-ilvD(Sm) + pRS411-Aft1 
                 2.60 ± 0.52 
               
               
                 pHR81-FBA-ilvD(Sm) + pRS411-Aft1(C291L) 
                 3.79 ± 0.23 
               
               
                 pHR81-FBA-ilvD(Sm) + pRS411-Aft1(L99A) 
                 5.41 ± 0.41 
               
               
                   
               
            
           
         
       
     
     Example 5 
     Increase in Cytosolic DHAD Specific Activity in a CCC1 Deletion Strain 
     The exact mechanism of increasing Fe—S cluster biosynthesis capability for cytosolic DHAD protein is unknown. Based on the findings with FRA2 and GRX3 deletion strains (Example 3) and with expression of Aft1p mutants (Example 4), increasing the availability of the Fe content in the cytosol may also improve the DHAD specific activity. CCC1 deletion has been shown to increase the Fe content of the cytosol (Li L, et al,  J Biol Chem.  276:29515-29519 (2001)). To test this hypothesis, the CCC1 deletion strain of BY4741 was transformed with plasmid pHR81 FBA-IlvD(Sm) as described in Example 1. The crude extracts of cells with the plasmid were assayed for DHAD activity. Table 13 shows the results of the experiment. When the CCC1 deletion strain with the DHAD plasmid was grown in SD medium lacking uracil, an increase in DHAD specific activity was found as compared to the wild-type cells with the same plasmid. When extra Fe was added, a further increase in DHAD was observed in the CCC1 deletion strain. Addition of Fe showed no effect on DHAD specific activity in the wild-type cells. To achieve an over expression of the DHAD protein, strains were grown in SD medium lacking leucine (Example 1). Under these conditions, an increase in DHAD specific activity was detected. 
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Expression of DHAD from  S. mutans  in the 
               
               
                 BY4741(Δccc1) strain. 
               
            
           
           
               
               
               
               
            
               
                 Strains 
                 Growth conditions 
                 No extra Fe 
                 100 uM Fe 
               
               
                   
               
               
                 Wild-type 
                 -Ura 
                 0.37 ± 0.03 
                 0.46 ± 0.04 
               
               
                 Δccc1 
                 -Ura 
                 0.83 ± 0.04 
                 1.24 ± 0.03 
               
               
                 Wild-type 
                 -Leu 
                 1.60 ± 0.17 
                 1.83 ± 0.31 
               
               
                 Δccc1 
                 -Leu 
                 2.53 ± 0.29 
                  2.7 ± 1.07 
               
               
                   
               
            
           
         
       
     
     Example 6 
     Improvement of Specific Activity of DHAD from  L. lactis  Expressed in Yeast 
     Examples 1-5 used the DHAD enzyme from  S. mutans  to identify novel ways to increase the specific activity of DHAD when expressed in yeast. In this example, we investigated the application of these methods to improve the specific activity of the DHAD enzyme from  L. lactis  (SEQ ID NO: 958). The IlvD gene from  L. lactis  (SEQ ID NO: 959) was cloned into the pHR81 vector under the control of the FBA promoter ( FIG. 11 ). The resulting construct pHR81 FBA-IlvD(L1)-ADHt ( FIG. 11 ; SEQ ID NO: 960) was transformed into strains with a deletion in either the FRA2 or GRX3 gene. To study the effect of the constitutive mutant Aft1p(L99A) on DHAD from  L. lactis , pHR81 FBA-IlvD(L1)-ADHt was co-transformed into yeast host along with vector pRS411-Aft1(L99A) (see Example 4). To over-express the IlvD gene, transformants were grown in yeast synthetic drop-out medium lacking leucine or lacking both leucine and methionine, depending on the strains. Enzymatic assay results are summarized in Table 14. Deletions in FRA2 and GRX3 genes increased the specific activity of the DHAD from  L. lactis  when expressed in yeast. In addition, expression of the Aft1 constitutive mutant L99A similarly increased the specific activity of the DHAD from  L. lactis . 
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Over-expression of bacterial DHAD from  L. lactis   
               
               
                 in  S. cerevisiae.   
               
            
           
           
               
               
               
            
               
                   
                 Strains 
                 Specific Activity (U/mg) 
               
               
                   
                   
               
               
                   
                 Wild-type 
                 0.23 ± 0.04 
               
               
                   
                 Δaft1 + Aft1(L99A) 
                 0.95 ± 0.31 
               
               
                   
                 Δfra2 
                 0.72 ± 0.04 
               
               
                   
                 Δgrx3 
                 0.96 ± 0.05 
               
               
                   
                   
               
            
           
         
       
     
     Example 7 
     Determining the Specific Activity of DHAD. (Assay Method) 
     Quantitation of the activity of proteins requiring Fe—S clusters can be done in an assay format. If the protein is an enzyme, such as DHAD, the activity is typically expressed in terms of units of activity. A unit of enzyme activity has been defined by the Enzyme Commission of the International Union of Biochemistry as the amount of enzyme that will catalyze the transformation of 1 micromole of the substrate per minute under standard conditions (International Union of Biochemistry, Report of the Commission on Enzymes, Oxford: Pergamon Press, 1961). Further, the term specific activity is defined as the units of activity in a given amount of enzyme. Thus, the specific activity is not directly measured but is calculated by dividing 1) the activity in units/ml of the enzyme sample by 2) the concentration of protein in that sample, so the specific activity is expressed as units/mg. The specific activity of a sample of pure, fully active enzyme is a characteristic of that enzyme. The specific activity of a sample of a mixture of proteins is a measure of the relative fraction of protein in that sample that is composed of the active enzyme of interest. DHAD activity can be measured spectrophotometrically in an end point assay using the 2,4-dinitrophenylhydrazine (2,4-DNPH) method as described in Flint, D. H. and M. H. Emptage,  J. Biol. Chem.  263:3558-64 (1988). In this assay, the 2,4-DNPH reacts with the keto group of the 2-ketoisovaleric acid product to form a hydrazone, which is detected by its absorbance at 550 nm. The assay buffer contains 50 mM Tris-HCl, 10 mM MgCl 2 , pH 8.0 (TM8 buffer). Sufficient 2,3-dihydroxyisovaleric acid is added to the assay buffer so that its final concentration in the assay mix is 10 mM. In each assay, an enzyme containing solution and sufficient substrate containing buffer are mixed so that the final volume is 1 ml. The assay mixture is normally incubated at 37° C. for 30 minutes. 
     The assay is stopped by adding 250 μl of 10% (W/V) trichloroacetic acid. A few minutes later, 500 μl of a saturated solution of 2,4-DNPH in 1 N HCl is added. The mixture is incubated at room temperature for at least 10 min to allow formation of the hydrazone. Next, 1.75 ml of NaOH is added to solubilize the hydrazone and to precipitate unreacted 2,4-DNPH. A few minutes after the NaOH is added, the assay tubes are placed in a sonicator bath for 10 min to degas. The tubes are then centrifuged in a desk top centrifuge at top speed for 2 min to sediment the precipitate. 
     The absorbance of the supernatant is then read at 550 nm within 1 hour. The absorbance of the sample assays minus the control assays are divided by 2600 (determined from an α-ketoisovaleric acid standard curve) to find the units of enzyme activity in the assay. This assay was used in the Examples described herein in which DHAD specific activity was determined. 
     Example 8 
     Purification and Characterization of DHAD from  S. mutans  Expressed in  E. coli    
     DHAD from  S. mutans  was purified and characterized as follows. Six liters of culture of the  E. coli  Turner strain harboring the pET28a plasmid containing the  S. mutans  ilvD gene were grown and induced with IPTG. The  S. mutans  DHAD was purified by breaking the cells with a sonicator in TM8 buffer (see Example 7), centrifuging the crude extract to remove cell debris, then loading the supernatant of the crude extract on a Q Sepharose (GE Healthcare) column and eluting the DHAD with an increasing concentration of NaCl in TM8 buffer. The fractions containing DHAD were pooled, brought to 1 M (NH 4 ) 2 SO 4 , and loaded onto a Phenyl-Sepharose column (GE Healthcare) equilibrated with 1 M (NH 4 ) 2 SO 4 . The DHAD was eluted with a decreasing concentration of (NH 4 ) 2 SO 4 . The fractions containing DHAD were pooled, concentrated to ≤10 ml, loaded onto a 35×600 cm Superdex-200 column (577 ml bed volume) (GE Healthcare) column, and eluted with TM8 buffer. As judged by SDS gels, the purity of the  S. mutans  DHAD eluted from the Superdex column was estimated to be ≥90%. 
     The UV-visible spectrum of the purified  S. mutans  DHAD is shown in  FIG. 3 . The number of peaks above 300 nm is typical of proteins with [2Fe-2S] clusters. The  S. mutans  DHAD was reduced with sodium dithionite, and its EPR spectra was measured at various temperatures.  FIG. 4  shows the EPR spectra measured at temperatures between 20° K and 70° K. The EPR spectrum of the  S. mutans  DHAD is measureable up to 70° K, which indicates that it contains a [2Fe-2S] cluster and not a [4Fe-4S] cluster because the EPR spectra of proteins containing [4Fe-4S] clusters are not observable at temperatures much above 10° K. 
     The exact protein content of the batch of purified  S. mutans  DHAD with the highest specific activity using the Bradford protein assay was determined by quantitative amino acid analysis. Combining the activity with the protein content gave a specific activity of 100 units/mg for this batch. The iron content of this batch determined by ICP-MS using methodology known in the art was 2 molecules of iron per molecule of DHAD. This is consistent with this batch of  S. mutans  DHAD containing a full complement of [2Fe-2S] clusters. 
     Example 9 
     Separating the Forms of DHAD in Yeast Crude Extract from Other Proteins in the Cell and from Each Other to Measure the Amount of DHAD Present 
     DHAD protein in yeast cells exists in the forms of dimers with two Fe—S clusters/dimer, one Fe—S cluster/dimer, and zero Fe—S clusters/dimer. A method to measure the concentration of these three forms of DHAD protein in yeast crude extracts was developed using a Mono Q column and a Source 15 PHE PE 4.6/100 column (both columns obtained from GE Healthcare), and is described below. 
     Frozen yeast cells were thawed, suspended in 50 mM Tris-HCl, 10 mM MgCl 2 , pH 8.0 (TM8), then broken by bead beating. The broken cells are centrifuged to remove the cell debris and generate the yeast crude extract. 
     The crude extract was loaded onto a 4 ml Mono Q column attached to an AKTA chromatographic system (GE Healthcare) with the A buffer being TM8 and B buffer being TM8 containing 0.5 M NaCl. The column was equilibrated with A buffer before the sample was loaded. The  S. mutans  DHAD bound to the Mono Q column under these conditions. After the sample was loaded onto the column, the column was washed with 10 mL of TM8 buffer, then the concentration of NaCl in the eluant was increased to 0.22 M NaCl. This was followed by a 30 mL linear gradient from 0.22 M to 0.35 M NaCl. During chromatography, the A 215  of the column eluate was monitored, and 1 mL fractions were collected. The fractions were assayed for DHAD activity. The sum of the activity of the DHAD in the fractions off the Mono Q column was close to that in the crude extract. Good separations using this column were obtained with as much as 5 mL of crude extract representing up to 1 g of yeast cell paste. The DHAD containing fractions were pooled and made 1.35 M in (NH 4 ) 2 SO 4  in preparation for chromatography on the PHE column. 
     The Source 15 PHE PE 4.6/100 column was also attached to an AKTA chromatographic system with the A buffer being TM8 containing 1.5 M (NH 4 ) 2 SO 4  and the B buffer being TM8. Before each run the column was equilibrated with 90% A. The pooled fractions from the Mono Q column made 1.35 M in (NH 4 ) 2 SO 4  were loaded onto the PHE column, and at this (NH 4 ) 2 SO 4  concentration, the DHAD bound to the column. During chromatography, the A 215  of the column eluate was monitored, and 1 mL fractions were collected. The DHAD eluted from the column in three peaks when the column was developed with a 30 mL decreasing linear gradient of (NH 4 ) 2 SO 4  from 1.35 M to 0 M. The area of each of the DHAD peaks was determined by integration. This elution scheme was found to be ideal for separating  S. mutans  DHAD from other yeast proteins that co-eluted with it off the Mono Q column. SDS gels run on fractions where the peaks eluted showed that well over 90% of the protein present in these peaks was DHAD when it was expressed at 1% of the soluble protein in yeast cells. The fractions containing each of the three DHAD peaks were pooled separately. Based on the UV-visible absorbent spectrum and the iron and sulfide contents of the DHAD in these peaks, it was determined that the first peak contained DHAD with two [2Fe-2S] clusters/dimers, the second peak contained DHAD with one [2Fe-2S] cluster/dimer, and the third peak contained DHAD with zero [2Fe-2S] clusters/dimers. Thus, in its native state, the  S. mutans  DHAD enzyme appears to exist as a dimer of two monomeric DHAD proteins. 
     A standard curve relating the amount of DHAD present in a sample to the sum of the area of the three DHAD peaks off the PHE column was obtained as follows. Crude extract from yeast cells containing no  S. mutans  DHAD was spiked with various amounts of purified  S. mutans  DHAD. These extracts were subjected to chromatography on the Mono Q and PHE columns as described above. The area under each of the three DHAD peaks was integrated. The sum of these areas was plotted against the amount of pure DHAD spiked into the yeast crude extracts. The plot was used to derive the following equation:
 
#μg DHAD in sample of crude extract=0.507×(summed area counts of the three DHAD peaks)
 
     The DHAD activity in a crude extract of yeast can be readily determined by the method described in Example 7. The amount of DHAD protein in yeast crude extracts can be determined by the procedure outlined in this Example. With this data, one can calculate the specific activity of the  S. mutans  DHAD protein per se in crude extracts according to the procedure in Example 10. 
     Example 10 
     Methods to Determine the Fraction of DHAD in Yeast Crude Extract Loaded with Fe—S Clusters 
     When a purified Fe—S cluster requiring protein contains a full complement of clusters, it will have a characteristic specific activity. As previously mentioned, for  S. mutans  DHAD this specific activity is 100 units/mg when it has a full complement of clusters. 
     A DHAD sample that has on average one Fe—S cluster/per dimer could contain some dimers with two clusters, some dimers with one cluster, and some dimers with no clusters. Alternatively, if cluster addition to a dimer is all or none and on average there is one Fe—S cluster/dimer in a sample, half of the DHAD dimers would have a full complement of clusters and half would be without clusters. From the results in Example 9, we know that all or none behavior is not followed by  S. mutans  DHAD because a species with one cluster per dimer can be isolated. We have found that dimers of  S. mutans  DHAD that have one Fe—S cluster have 1/2 the activity of dimers with two Fe—S clusters/dimer, i.e., the specific activity of  S. mutans  DHAD with 1/2 of a full complement of Fe—S clusters is 50 units/mg. This means the absence of an Fe—S cluster in one of the monomers of a dimer does not influence the activity of the other monomer should it contain an Fe—S cluster. 
     With the information obtained with the procedures described in Example 9 and the information described so far in this Example, one can determine the degree of Fe—S cluster loading in a DHAD sample in two different ways. 
     First, one can compare the ratio of the amounts of the three DHAD peaks to determine the relative amount that has two clusters per dimer, one cluster per dimer, and zero clusters per dimer. This gives the degree of cluster loading. For example, if the area of peak 1 off the PHE column was 25%, peak 2 was 50%, and peak 3 was 25% of the sum of the areas of peak 1, peak 2, and peak 3, the percent of the monomers loaded with clusters can be calculated to be 50% according to the following equation:
 
100*[2*(area of peak 1)+1*(area of peak 2)+0*(area of peak 3)]/[2*(total peak area)]=% DHAD monomers with Fe—S clusters.
 
     Second, one can use the specific activity of the DHAD present to calculate the degree of cluster loading. One determines the specific activity by dividing the activity determined as described in Example 7 with the amount of DHAD protein determined as described in Example 9. The specific activity is then divided by 100 U/mg to determine the fraction of monomers loaded with clusters. This fraction is multiplied by 100 to determine the percent DHAD monomers with Fe—S clusters. 
     For example if the specific activity is found to be 50 U/mg, the fraction loaded with clusters is 0.5 and the percent DHAD monomers with Fe—S clusters is 50%. 
     To make such a calculation, the specific activity must be based on the concentration of the DHAD protein in the crude extract (not the total protein). Determining the concentration of  S. mutans  DHAD in the presence of other proteins can be accomplished using methods described in Example 9. 
     Example 11 
     Specific Activities and Inferred Fraction of the DHAD-Loaded Proteins 
     To determine the fraction of DHAD monomers loaded with Fe—S clusters in several yeast strains grown under different conditions, the methods described above were used. Results are shown in Table 15. 
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 Specific Activities and Inferred Fraction of the DHAD 
               
               
                 Loaded Proteins. 
               
            
           
           
               
               
               
               
               
            
               
                 BY 
                   
                 DHAD SA in 
                 % DHAD is of 
                 % Cluster 
               
               
                 Yeast 
                 Growth 
                 Crude Extracts 
                 Crude Extract 
                 Occupancy of 
               
               
                 Strain 
                 Conditions 
                 (U/mg) 
                 Protein 
                 DHAD 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 WT 
                 -Ura 
                 0.46 
                 2.3 
                 10 
               
               
                 ΔFRA2 
                 -Ura 
                 0.8 
                 2.5 
                 14 
               
               
                 ΔGRX3 
                 -Ura 
                 0.99 
                 2.4 
                 23 
               
               
                 WT 
                 -Leu 
                 0.82 
                 11 
                 7 
               
               
                 ΔFRA2 
                 -Leu 
                 2.2 
                 11 
                 19 
               
               
                 ΔGRX3 
                 -Leu 
                 3.5 
                 9.5 
                 31 
               
               
                   
               
            
           
         
       
     
     These results indicate that under these growth conditions, the level of Fe—S cluster loading in the DHAD in strains lacking FRA2 and GRX3 is higher than in strains containing functional copies of these genes. Thus, a higher fraction of the DHAD protein is in the active form in the deletion strains because the content of Fe—S clusters (which are required for activity) is higher. 
     Example 12 
     Construction of  Saccharomyces cerevisiae  Strains PNY1505, PNY1541, and PNY1542 
     The purpose of this Example was to construct  Saccharomyces cerevisiae  strains PNY1505, PNY1541, and PNY1542. These strains were derived from PNY1503 (BP1064). PNY1503 was derived from CEN.PK 113-7D (CBS 8340; Centraalbureau voor Schimmelcultures (CBS) Fungal Biodiversiry Centre, Netherlands). The construction of PNY1503 (BP1064) is described in U.S. Appl. No. 61/368,436, incorporated by reference herein, and in Example 13 below. PNY1505 contains a deletion of the FRA2 gene. PNY1541 and PNY1542 contain an integration of the AFT/gene with the L99A mutation (AFT1-L99A) at the YPRCΔ15 locus. 
     Deletions/integrations were created by homologous recombination with PCR fragments containing regions of homology upstream and downstream of the target gene and the URA3 gene for selection of transformants. The URA3 gene was removed by homologous recombination to create a scarless deletion/integration. 
     The scarless deletion/integration procedure was adapted from Akada et al.,  Yeast,  23(5):399-405 (2006). The PCR cassette for each deletion/integration was made by combining four fragments, A-B-U-C, either by overlapping PCR or by cloning the individual fragments, and gene to be integrated, into a plasmid prior to amplifying the entire cassette by PCR for the deletion/integration procedure. The PCR cassette contained a selectable/counter-selectable marker, URA3 (Fragment U), consisting of the native CEN.PK 113-7D URA3 gene, along with the promoter (250 bp upstream of the URA3 gene) and terminator (150 bp downstream of the URA3 gene) regions. Fragments A (150 bp to 500 bp long) and C (250 bp long) corresponded to the sequence immediately upstream of the target gene (Fragment A) and the 3′ sequence of the target gene (Fragment C). Fragments A and C were used for integration of the cassette into the chromosome by homologous recombination. Fragment B (500 bp long) corresponded to the 500 bp immediately downstream of the target gene and was used for excision of the URA3 marker and Fragment C from the chromosome by homologous recombination, as a direct repeat of the sequence corresponding to Fragment B was created upon integration of the cassette into the chromosome. 
     Using the PCR product ABUC cassette, the URA3 marker was first integrated into and then excised from the chromosome by homologous recombination. The initial integration deleted the gene, excluding the 3′ sequence. Upon excision, the 3′ region of the gene was also deleted. For integration of genes using this method, the gene to be integrated was included in the cassette between fragments A and B. 
     FRA2 Deletion 
     The FRA2 deletion (also described in U.S. Appl. No. 61/380,563, incorporated by reference herein) was designed to delete 250 nucleotides from the 3′ end of the coding sequence, leaving the first 113 nucleotides of the FRA2 coding sequence intact. An in-frame stop codon was present 7 nucleotides downstream of the deletion. The four fragments for the PCR cassette for the scarless FRA2 deletion were amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs; Ipswich, Mass.) and CEN.PK 113-7D genomic DNA as template, prepared with a Gentra Puregene Yeast/Bact kit (Qiagen; Valencia, Calif.). FRA2 Fragment A was amplified with primer oBP594 (SEQ ID NO: 961) and primer oBP595 (SEQ ID NO: 962), containing a 5′ tail with homology to the 5′ end of FRA2 Fragment B. FRA2 Fragment B was amplified with primer oBP596 (SEQ ID NO: 963), containing a 5′ tail with homology to the 3′ end of FRA2 Fragment A, and primer oBP597 (SEQ ID NO: 964), containing a 5′ tail with homology to the 5′ end of FRA2 Fragment U. FRA2 Fragment U was amplified with primer oBP598 (SEQ ID NO: 965), containing a 5′ tail with homology to the 3′ end of FRA2 Fragment B, and primer oBP599 (SEQ ID NO: 966), containing a 5′ tail with homology to the 5′ end of FRA2 Fragment C. FRA2 Fragment C was amplified with primer oBP600 (SEQ ID NO: 967), containing a 5′ tail with homology to the 3′ end of FRA2 Fragment U, and primer oBP601 (SEQ ID NO: 968). PCR products were purified with a PCR Purification kit (Qiagen). FRA2 Fragment AB was created by overlapping PCR by mixing FRA2 Fragment A and FRA2 Fragment B and amplifying with primers oBP594 (SEQ ID NO: 961) and oBP597 (SEQ ID NO: 964). FRA2 Fragment UC was created by overlapping PCR by mixing FRA2 Fragment U and FRA2 Fragment C and amplifying with primers oBP598 (SEQ ID NO: 965) and oBP601 (SEQ ID NO: 968). The resulting PCR products were purified on an agarose gel followed by a Gel Extraction kit (Qiagen). The FRA2 ABUC cassette was created by overlapping PCR by mixing FRA2 Fragment AB and FRA2 Fragment UC and amplifying with primers oBP594 (SEQ ID NO: 961) and oBP601 (SEQ ID NO: 968). The PCR product was purified with a PCR Purification kit (Qiagen). 
     Competent cells of PNY1503 were made and transformed with the FRA2 ABUC PCR cassette using a Frozen-EZ Yeast Transformation II kit (Zymo Research; Orange, Calif.). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 1% ethanol at 30° C. Transformants with a fra2 knockout were screened for by PCR with primers oBP602 (SEQ ID NO: 969) and oBP603 (SEQ ID NO: 970) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). A correct transformant was grown in YPE (yeast extract, peptone, 1% ethanol) and plated on synthetic complete medium supplemented with 1% ethanol and containing 5-fluoro-orotic acid (0.1%) at 30° C. to select for isolates that lost the URA3 marker. The deletion and marker removal were confirmed by PCR with primers oBP602 (SEQ ID NO: 969) and oBP603 (SEQ ID NO: 970) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The absence of the FRA2 gene from the isolate was demonstrated by a negative PCR result using primers specific for the deleted coding sequence of FRA2, oBP605 (SEQ ID NO: 971) and oBP606 (SEQ ID NO: 972). The correct isolate was selected as strain CEN.PK 113-7D MATa ura3A::loxP his3Δ pdc6Δ pdc1Δ::P[PDC1]-DHAD|ilvD_Sm-PDC1t pdc5Δ::P[PDC5]-ADH|sadB_Ax-PDC5t gpd2Δ::loxP fra2Δ and designated as PNY1505 (BP1135). 
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Primers used in the FRA2 Deletion 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 SEQ 
                   
               
               
                   
                 Primer 
                 ID 
                   
               
               
                   
                 Name 
                 NO 
                 Primer Sequence 
               
               
                   
                   
               
               
                   
                 oBP594 
                 961 
                 agctgtctcgtgttgtgggttt 
               
               
                   
                   
               
               
                   
                 oBP595 
                 962 
                 cttaataatagaacaatatcatcct 
               
               
                   
                   
                   
                 ttacgggcatcttatagtgtcgtt 
               
               
                   
                   
               
               
                   
                 oBP596 
                 963 
                 gcgccaacgacactataagatgccc 
               
               
                   
                   
                   
                 gtaaaggatgatattgttctatta 
               
               
                   
                   
               
               
                   
                 oBP597 
                 964 
                 tatggaccctgaaaccacagccaca 
               
               
                   
                   
                   
                 ttgcaacgacgacaatgccaaacc 
               
               
                   
                   
               
               
                   
                 oBP598 
                 965 
                 tccttggtttggcattgtcgtcgtt 
               
               
                   
                   
                   
                 gcaatgtggctgtggtttcagggt 
               
               
                   
                   
               
               
                   
                 oBP599 
                 966 
                 atcctctcgcggagtccctgttcag 
               
               
                   
                   
                   
                 taaaggccatgaagctttttcttt 
               
               
                   
                   
               
               
                   
                 oBP600 
                 967 
                 attggaaagaaaaagcttcatggcc 
               
               
                   
                   
                   
                 tttactgaacagggactccgcgag 
               
               
                   
                   
               
               
                   
                 oBP601 
                 968 
                 tcataccacaatcttagaccat 
               
               
                   
                   
               
               
                   
                 oBP602 
                 969 
                 tgttcaaacccctaaccaacc 
               
               
                   
                   
               
               
                   
                 oBP603 
                 970 
                 tgttcccacaatctattaccta 
               
               
                   
                   
               
               
                   
                 oBP605 
                 971 
                 tactgaacagggactccgcga 
               
               
                   
                   
               
               
                   
                 oBP606 
                 972 
                 tcataccacaatcttagacca 
               
               
                   
                   
               
            
           
         
       
     
     YPRCΔ15 Deletion and AFT1-L99A Integration 
     The YPRCΔ15 locus was deleted and replaced with AFT1-L99A along with the native promoter region (800 bp) and terminator region (800 bp) from AFT1. The scarless cassette for the YPRCΔ15 deletion-AFT1L99A integration was first cloned into plasmid pUC19-URA3MCS (described in U.S. Appl. No. 61/356,379, incorporated by reference herein). The vector is pUC19 based and contains the sequence of the URA3 gene from  S. cerevisiae  CEN.PK 113-7D situated within a multiple cloning site (MCS). pUC19 (American Type Culture Collection, Manassas, Va.; ATCC #37254) contains the pMB1 replicon and a gene coding for beta-lactamase for replication and selection in  Escherichia coli . In addition to the coding sequence for URA3, the sequences from upstream (250 bp) and downstream (150 bp) of this gene are present for expression of the URA3 gene in yeast. The vector can be used for cloning purposes and can be used as a yeast integration vector. 
     The DNA encompassing the URA3 coding region along with 250 bp upstream and 150 bp downstream of the URA3 coding region from  Saccharomyces cerevisiae  CEN.PK 113-7D (CBS 8340; Centraalbureau voor Schimmelcultures (CBS) Fungal Biodiversity Centre, Netherlands) genomic DNA was amplified with primers oBP438 (SEQ ID NO: 1033), containing BamHI, AscI, PmeI, and FseI restriction sites, and oBP439 (SEQ ID NO: 1034), containing XbaI, Pad, and Nod restriction sites. Genomic DNA was prepared using a Gentra Puregene Yeast/Bact kit (Qiagen). The PCR product and pUC19 were ligated with T4 DNA ligase after digestion with BamHI and XbaI to create vector pUC19-URA3MCS. The vector was confirmed by PCR and sequencing with primers oBP264 (SEQ ID NO:1031) and oBP265 (SEQ ID NO: 1032). 
     YPRCΔ15 Fragment A was amplified from genomic DNA, prepared as above, with primer oBP622 (SEQ ID NO: 973), containing a KpnI restriction site, and primer oBP623 (SEQ ID NO: 974), containing a 5′ tail with homology to the 5′ end of YPRCΔ15 Fragment B. YPRCΔ15 Fragment B was amplified from genomic DNA with primer oBP624 (SEQ ID NO: 975), containing a 5′ tail with homology to the 3′ end of YPRCΔ15 Fragment A, and primer oBP625 (SEQ ID NO: 976), containing a FseI restriction site. PCR products were purified with a PCR Purification kit (Qiagen). YPRCΔ15 Fragment A-YPRCΔ15 Fragment B was created by overlapping PCR by mixing the YPRCΔ15 Fragment A and YPRCΔ15 Fragment B PCR products and amplifying with primers oBP622 (SEQ ID NO: 973) and oBP625 (SEQ ID NO: 976). The resulting PCR product was digested with KpnI and FseI and ligated with T4 DNA ligase into the corresponding sites of pUC19-URA3MCS after digestion with the appropriate enzymes. YPRCΔ15 Fragment C was amplified from genomic DNA with primer oBP626 (SEQ ID NO: 977), containing a NotI restriction site, and primer oBP627 (SEQ ID NO: 978), containing a PacI restriction site. The YPRCΔ15 Fragment C PCR product was digested with NotI and PacI and ligated with T4 DNA ligase into the corresponding sites of the plasmid containing YPRCΔ15 Fragments AB. AFT1-L99A, along with the native promoter region (800 bp) and terminator region (800 bp) from AFT1, was amplified using pRS411-AFT1(L99A) (described in Example 4 above) as template with primer oBP744 (SEQ ID NO: 979), containing an AscI restriction site, and primer oBP745 (SEQ ID NO: 980), containing a PmeI restriction site. The PCR product was digested with AscI and PmeI and ligated with T4 DNA ligase into the corresponding sites of the plasmid containing YPRCΔ15 Fragments ABC. The entire integration cassette was amplified from the resulting plasmid with primers oBP622 (SEQ ID NO: 973) and oBP627 (SEQ ID NO: 978). 
     Competent cells of PNY1503 were made and transformed with the YPRCΔ15 deletion/AFT1-L99A integration cassette PCR product using a Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 1% ethanol at 30° C. Transformants were grown in YPE (1% ethanol) and plated on synthetic complete medium supplemented with 1% EtOH and containing 5-fluoro-orotic acid (0.1%) at 30° C. to select for isolates that lost the URA3 marker. The deletion of YPRCΔ15 and integration of AFT1L99A were confirmed by PCR with external primers oBP636 (SEQ ID NO: 981) and oBP637 (SEQ ID NO: 982) and with AFT1-L99A specific primer HY840 (SEQ ID NO: 983) and external primer oBP637 (SEQ ID NO: 982) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen) and by colony PCR. Correct independent isolates of CEN.PK 113-7D MATa ura3Δ::loxP his3Δ pdc6Δ pdc1Δ::P[PDC1]-DHAD|ilvD_Sm-PDC1t pdc5Δ::P[PDC5]-ADH|sadB_Ax-PDC5t gpd2Δ::loxP yprcΔ15Δ::AFT1L99A were designated as strains PNY1541 and PNY1542. 
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 Primers used in the YPRCΔ15 Deletion and 
               
               
                 AFT1-L99A Integration 
               
            
           
           
               
               
               
            
               
                   
                 SEQ 
                   
               
               
                 Primer 
                 ID 
                   
               
               
                 Name 
                 NO 
                 Primer Sequence 
               
               
                   
               
               
                 oBP622 
                 973 
                 aattggtaccccaaaaggaatattgggtcaga 
               
               
                   
               
               
                 oBP623 
                 974 
                 ccattgtttaaacggcgcgccggatcctttgc 
               
               
                   
                   
                 gaaaccctatgctctgt 
               
               
                   
               
               
                 oBP624 
                 975 
                 gcaaaggatccggcgcgccgtttaaacaatgg 
               
               
                   
                   
                 aaggtcgggatgagcat 
               
               
                   
               
               
                 oBP625 
                 976 
                 aattggccggcctacgtaacattctgtcaaccaa 
               
               
                   
               
               
                 oBP626 
                 977 
                 aattgcggccgcttcatatatgacgtaataaaat 
               
               
                   
               
               
                 oBP627 
                 978 
                 aattttaattaattttttttcttggaatcagtac 
               
               
                   
               
               
                 oBP744 
                 979 
                 aattggcgcgccagagtacaacgatcaccgcctg 
               
               
                   
               
               
                 oBP745 
                 980 
                 aattgtttaaacgaacgaaagttacaaaatctag 
               
               
                   
               
               
                 oBP636 
                 981 
                 catttttttccctctaagaagc 
               
               
                   
               
               
                 oBP637 
                 982 
                 tttttgcacagttaaactaccc 
               
               
                   
               
               
                 HY840 
                 983 
                 CCAAAATCAGCCCCACGACGGCCATA 
               
               
                   
               
            
           
         
       
     
     Example 13 
     Construction of  Saccharomyces cerevisiae  Strain BP1064 (PNY1503) 
     The strain BP1064 was derived from CEN.PK 113-7D (CBS 8340; Centraalbureau voor Schimmelcultures (CBS) Fungal Biodiversity Centre, Netherlands) and contains deletions of the following genes: URA3, HIS3, PDC1, PDC5, PDC6, and GPD2. 
     Deletions, which completely removed the entire coding sequence, were created by homologous recombination with PCR fragments containing regions of homology upstream and downstream of the target gene and either a G418 resistance marker or URA3 gene for selection of transformants. The G418 resistance marker, flanked by loxP sites, was removed using Cre recombinase. The URA3 gene was removed by homologous recombination to create a scarless deletion, or if flanked by loxP sites was removed using Cre recombinase. 
     The scarless deletion procedure was adapted from Akada et al. 2006 Yeast v23 p399. In general, the PCR cassette for each scarless deletion was made by combining four fragments, A-B-U-C, by overlapping PCR. The PCR cassette contained a selectable/counter-selectable marker, URA3 (Fragment U), consisting of the native CEN.PK 113-7D URA3 gene, along with the promoter (250 bp upstream of the URA3 gene) and terminator (150 bp downstream of the URA3 gene). Fragments A and C, each 500 bp long, corresponded to the 500 bp immediately upstream of the target gene (Fragment A) and the 3′ 500 bp of the target gene (Fragment C). Fragments A and C were used for integration of the cassette into the chromosome by homologous recombination. Fragment B (500 bp long) corresponded to the 500 bp immediately downstream of the target gene and was used for excision of the URA3 marker and Fragment C from the chromosome by homologous recombination, as a direct repeat of the sequence corresponding to Fragment B was created upon integration of the cassette into the chromosome. Using the PCR product ABUC cassette, the URA3 marker was first integrated into and then excised from the chromosome by homologous recombination. The initial integration deleted the gene, excluding the 3′ 500 bp. Upon excision, the 3′ 500 bp region of the gene was also deleted. For integration of genes using this method, the gene to be integrated was included in the PCR cassette between fragments A and B. 
     URA3 Deletion 
     To delete the endogenous URA3 coding region, a ura3::loxP-kanMX-loxP cassette was PCR-amplified from pLA54 template DNA (SEQ ID NO: 986). pLA54 contains the  K. lactis  TEF1 promoter and kanMX marker, and is flanked by loxP sites to allow recombination with Cre recombinase and removal of the marker. PCR was done using Phusion DNA polymerase and primers BK505 and BK506 (SEQ ID NOs: 987 and 988, respectively). The URA3 portion of each primer was derived from the 5′ region upstream of the URA3 promoter and 3′ region downstream of the coding region such that integration of the loxP-kanMX-loxP marker resulted in replacement of the URA3 coding region. The PCR product was transformed into CEN.PK 113-7D using standard genetic techniques ( Methods in Yeast Genetics,  2005, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp. 201-202) and transformants were selected on YPD containing G418 (100 μg/ml) at 30 C. Transformants were screened to verify correct integration by PCR using primers LA468 and LA492 (SEQ ID NOs: 989 and 990, respectively) and designated CEN.PK 113-7D Δura3::kanMX. 
     HIS3 Deletion 
     The four fragments for the PCR cassette for the scarless HIS3 deletion were amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs; Ipswich, Mass.) and CEN.PK 113-7D genomic DNA as template, prepared with a Gentra Puregene Yeast/Bact kit (Qiagen; Valencia, Calif.). HIS3 Fragment A was amplified with primer oBP452 (SEQ ID NO: 991) and primer oBP453 (SEQ ID NO: 992), containing a 5′ tail with homology to the 5′ end of HIS3 Fragment B. HIS3 Fragment B was amplified with primer oBP454 (SEQ ID NO: 993), containing a 5′ tail with homology to the 3′ end of HIS3 Fragment A, and primer oBP455 (SEQ ID NO: 994), containing a 5′ tail with homology to the 5′ end of HIS3 Fragment U. HIS3 Fragment U was amplified with primer oBP456 (SEQ ID NO: 995), containing a 5′ tail with homology to the 3′ end of HIS3 Fragment B, and primer oBP457 (SEQ ID NO: 996), containing a 5′ tail with homology to the 5′ end of HIS3 Fragment C. HIS3 Fragment C was amplified with primer oBP458 (SEQ ID NO: 997), containing a 5′ tail with homology to the 3′ end of HIS3 Fragment U, and primer oBP459 (SEQ ID NO: 998). PCR products were purified with a PCR Purification kit (Qiagen). HIS3 Fragment AB was created by overlapping PCR by mixing HIS3 Fragment A and HIS3 Fragment B and amplifying with primers oBP452 (SEQ ID NO: 991) and oBP455 (SEQ ID NO: 994). HIS3 Fragment UC was created by overlapping PCR by mixing HIS3 Fragment U and HIS3 Fragment C and amplifying with primers oBP456 (SEQ ID NO: 995) and oBP459 (SEQ ID NO: 998). The resulting PCR products were purified on an agarose gel followed by a Gel Extraction kit (Qiagen). The HIS3 ABUC cassette was created by overlapping PCR by mixing HIS3 Fragment AB and HIS3 Fragment UC and amplifying with primers oBP452 (SEQ ID NO: 991) and oBP459 (SEQ ID NO: 998). The PCR product was purified with a PCR Purification kit (Qiagen). 
     Competent cells of CEN.PK 113-7D Δura3::kanMX were made and transformed with the HIS3 ABUC PCR cassette using a Frozen-EZ Yeast Transformation II kit (Zymo Research; Orange, Calif.). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 2% glucose at 30° C. Transformants with a his3 knockout were screened for by PCR with primers oBP460 (SEQ ID NO: 999) and oBP461 (SEQ ID NO: 1000) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). A correct transformant was selected as strain CEN.PK 113-7D Δura3::kanMX Δhis3::URA3. 
     KanMX Marker Removal from the ΔUra3 Site and URA3 Marker Removal from the Δhis3 Site 
     The KanMX marker was removed by transforming CEN.PK 113-7D Δura3::kanMX Δhis3::URA3 with pRS423::PGAL1-cre (SEQ ID NO: 1011, described in U.S. Provisional Application No. 61/290,639) using a Frozen-EZ Yeast Transformation II kit (Zymo Research) and plating on synthetic complete medium lacking histidine and uracil supplemented with 2% glucose at 30° C. Transformants were grown in YP supplemented with 1% galactose at 30° C. for ˜6 hours to induce the Cre recombinase and KanMX marker excision and plated onto YPD (2% glucose) plates at 30° C. for recovery. An isolate was grown overnight in YPD and plated on synthetic complete medium containing 5-fluoro-orotic acid (0.1%) at 30° C. to select for isolates that lost the URA3 marker. 5-FOA resistant isolates were grown in and plated on YPD for removal of the pRS423::P GAL1 -cre plasmid. Isolates were checked for loss of the KanMX marker, URA3 marker, and pRS423::P GAL1 -cre plasmid by assaying growth on YPD+G418 plates, synthetic complete medium lacking uracil plates, and synthetic complete medium lacking histidine plates. A correct isolate that was sensitive to G418 and auxotrophic for uracil and histidine was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 and designated as BP857. The deletions and marker removal were confirmed by PCR and sequencing with primers oBP450 (SEQ ID NO: 1001) and oBP451 (SEQ ID NO: 1002) for Δura3 and primers oBP460 (SEQ ID NO: 999) and oBP461 (SEQ ID NO: 1000) for Δhis3 using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). 
     PDC6 Deletion 
     The four fragments for the PCR cassette for the scarless PDC6 deletion were amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs) and CEN.PK 113-7D genomic DNA as template, prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). PDC6 Fragment A was amplified with primer oBP440 (SEQ ID NO: 1003) and primer oBP441 (SEQ ID NO: 1004), containing a 5′ tail with homology to the 5′ end of PDC6 Fragment B. PDC6 Fragment B was amplified with primer oBP442 (SEQ ID NO: 1005), containing a 5′ tail with homology to the 3′ end of PDC6 Fragment A, and primer oBP443 (SEQ ID NO: 1006), containing a 5′ tail with homology to the 5′ end of PDC6 Fragment U. PDC6 Fragment U was amplified with primer oBP444 (SEQ ID NO: 1007), containing a 5′ tail with homology to the 3′ end of PDC6 Fragment B, and primer oBP445 (SEQ ID NO: 1008), containing a 5′ tail with homology to the 5′ end of PDC6 Fragment C. PDC6 Fragment C was amplified with primer oBP446 (SEQ ID NO: 1009), containing a 5′ tail with homology to the 3′ end of PDC6 Fragment U, and primer oBP447 (SEQ ID NO: 1010). PCR products were purified with a PCR Purification kit (Qiagen). PDC6 Fragment AB was created by overlapping PCR by mixing PDC6 Fragment A and PDC6 Fragment B and amplifying with primers oBP440 (SEQ ID NO: 1003) and oBP443 (SEQ ID NO: 1006). PDC6 Fragment UC was created by overlapping PCR by mixing PDC6 Fragment U and PDC6 Fragment C and amplifying with primers oBP444 (SEQ ID NO: 1007) and oBP447 (SEQ ID NO: 1010). The resulting PCR products were purified on an agarose gel followed by a Gel Extraction kit (Qiagen). The PDC6 ABUC cassette was created by overlapping PCR by mixing PDC6 Fragment AB and PDC6 Fragment UC and amplifying with primers oBP440 (SEQ ID NO: 1003) and oBP447 (SEQ ID NO: 1010). The PCR product was purified with a PCR Purification kit (Qiagen). 
     Competent cells of CEN.PK 113-7D Δura3::loxP Δhis3 were made and transformed with the PDC6 ABUC PCR cassette using a Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 2% glucose at 30° C. Transformants with a pdc6 knockout were screened for by PCR with primers oBP448 (SEQ ID NO: 1012) and oBP449 (SEQ ID NO: 1013) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). A correct transformant was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6::URA3. 
     CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6::URA3 was grown overnight in YPD and plated on synthetic complete medium containing 5-fluoro-orotic acid (0.1%) at 30° C. to select for isolates that lost the URA3 marker. The deletion and marker removal were confirmed by PCR and sequencing with primers oBP448 (SEQ ID NO: 1012) and oBP449 (SEQ ID NO: 1013) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The absence of the PDC6 gene from the isolate was demonstrated by a negative PCR result using primers specific for the coding sequence of PDC6, oBP554 (SEQ ID NO: 1014) and oBP555 (SEQ ID NO: 1015). The correct isolate was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 and designated as BP891. 
     PDC1 Deletion ilvDSm Integration 
     The PDC1 gene was deleted and replaced with the ilvD coding region from  Streptococcus mutans  ATCC #700610. The A fragment followed by the ilvD coding region from  Streptococcus mutans  for the PCR cassette for the PDC1 deletion-ilvDSm integration was amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs) and NYLA83 (described in U.S. Provisional Application No. 61/246,709) genomic DNA as template, prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). PDC1 Fragment A-ilvDSm (SEQ ID NO: 1053) was amplified with primer oBP513 (SEQ ID NO: 1016) and primer oBP515 (SEQ ID NO: 1017), containing a 5′ tail with homology to the 5′ end of PDC1 Fragment B. The B, U, and C fragments for the PCR cassette for the PDC1 deletion-ilvDSm integration were amplified using Phusion High Fidelity PCR Master Mix (New England BioLabs) and CEN.PK 113-7D genomic DNA as template, prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). PDC1 Fragment B was amplified with primer oBP516 (SEQ ID NO: 1018) containing a 5′ tail with homology to the 3′ end of PDC1 Fragment A-ilvDSm, and primer oBP517 (SEQ ID NO: 1019), containing a 5′ tail with homology to the 5′ end of PDC1 Fragment U. PDC1 Fragment U was amplified with primer oBP518 (SEQ ID NO: 1020), containing a 5′ tail with homology to the 3′ end of PDC1 Fragment B, and primer oBP519 (SEQ ID NO: 1021), containing a 5′ tail with homology to the 5′ end of PDC1 Fragment C. PDC1 Fragment C was amplified with primer oBP520 (SEQ ID NO: 1022), containing a 5′ tail with homology to the 3′ end of PDC1 Fragment U, and primer oBP521 (SEQ ID NO: 1023). PCR products were purified with a PCR Purification kit (Qiagen). PDC1 Fragment A-ilvDSm-B was created by overlapping PCR by mixing PDC1 Fragment A-ilvDSm and PDC1 Fragment B and amplifying with primers oBP513 (SEQ ID NO: 1016) and oBP517 (SEQ ID NO: 1019). PDC1 Fragment UC was created by overlapping PCR by mixing PDC1 Fragment U and PDC1 Fragment C and amplifying with primers oBP518 (SEQ ID NO: 1020) and oBP521 (SEQ ID NO: 1023). The resulting PCR products were purified on an agarose gel followed by a Gel Extraction kit (Qiagen). The PDC1 A-ilvDSm-BUC cassette (SEQ ID NO: 1054) was created by overlapping PCR by mixing PDC1 Fragment A-ilvDSm-B and PDC1 Fragment UC and amplifying with primers oBP513 (SEQ ID NO: 1016) and oBP521 (SEQ ID NO: 1023). The PCR product was purified with a PCR Purification kit (Qiagen). 
     Competent cells of CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 were made and transformed with the PDC1 A-ilvDSm-BUC PCR cassette using a Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 2% glucose at 30° C. Transformants with a pdc1 knockout ilvDSm integration were screened for by PCR with primers oBP511 (SEQ ID NO: 1024) and oBP512 (SEQ ID NO: 1025) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The absence of the PDC1 gene from the isolate was demonstrated by a negative PCR result using primers specific for the coding sequence of PDC1, oBP550 (SEQ ID NO: 1026) and oBP551 (SEQ ID NO: 1027). A correct transformant was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm-URA3. 
     CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm-URA3 was grown overnight in YPD and plated on synthetic complete medium containing 5-fluoro-orotic acid (0.1%) at 30° C. to select for isolates that lost the URA3 marker. The deletion of PDC1, integration of ilvDSm, and marker removal were confirmed by PCR and sequencing with primers oBP511 (SEQ ID NO: 1024) and oBP512 (SEQ ID NO: 1025) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The correct isolate was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm and designated as BP907. 
     PDC5 Deletion sadB Integration 
     The PDC5 gene was deleted and replaced with the sadB coding region from  Achromobacter xylosoxidans . A segment of the PCR cassette for the PDC5 deletion-sadB integration was first cloned into plasmid pUC19-URA3MCS. 
     pUC19-URA3MCS is pUC19 based and contains the sequence of the URA3 gene from  Saccharomyces cerevisiae  situated within a multiple cloning site (MCS). pUC19 contains the pMB1 replicon and a gene coding for beta-lactamase for replication and selection in  Escherichia coli . In addition to the coding sequence for URA3, the sequences from upstream and downstream of this gene were included for expression of the URA3 gene in yeast. The vector can be used for cloning purposes and can be used as a yeast integration vector. 
     The DNA encompassing the URA3 coding region along with 250 bp upstream and 150 bp downstream of the URA3 coding region from  Saccharomyces cerevisiae  CEN.PK 113-7D genomic DNA was amplified with primers oBP438 (SEQ ID NO: 1033), containing BamHI, AscI, PmeI, and FseI restriction sites, and oBP439 (SEQ ID NO: 1034), containing XbaI, Pad, and NotI restriction sites, using Phusion High-Fidelity PCR Master Mix (New England BioLabs). Genomic DNA was prepared using a Gentra Puregene Yeast/Bact kit (Qiagen). The PCR product and pUC19 (SEQ ID NO: 1056) were ligated with T4 DNA ligase after digestion with BamHI and XbaI to create vector pUC19-URA3MCS. The vector was confirmed by PCR and sequencing with primers oBP264 (SEQ ID NO: 1031) and oBP265 (SEQ ID NO: 1032). 
     The coding sequence of sadB and PDC5 Fragment B were cloned into pUC19-URA3MCS to create the sadB-BU portion of the PDC5 A-sadB-BUC PCR cassette. The coding sequence of sadB was amplified using pLH468-sadB (SEQ ID NO: 1051) as template with primer oBP530 (SEQ ID NO: 1035), containing an AscI restriction site, and primer oBP531 (SEQ ID NO: 1036), containing a 5′ tail with homology to the 5′ end of PDC5 Fragment B. PDC5 Fragment B was amplified with primer oBP532 (SEQ ID NO: 1037), containing a 5′ tail with homology to the 3′ end of sadB, and primer oBP533 (SEQ ID NO: 1038), containing a PmeI restriction site. PCR products were purified with a PCR Purification kit (Qiagen). sadB-PDC5 Fragment B was created by overlapping PCR by mixing the sadB and PDC5 Fragment B PCR products and amplifying with primers oBP530 (SEQ ID NO: 1035) and oBP533 (SEQ ID NO: 1038). The resulting PCR product was digested with AscI and PmeI and ligated with T4 DNA ligase into the corresponding sites of pUC19-URA3MCS after digestion with the appropriate enzymes. The resulting plasmid was used as a template for amplification of sadB-Fragment B-Fragment U using primers oBP536 (SEQ ID NO: 1039) and oBP546 (SEQ ID NO: 1040), containing a 5′ tail with homology to the 5′ end of PDC5 Fragment C. PDC5 Fragment C was amplified with primer oBP547 (SEQ ID NO: 1041) containing a 5′ tail with homology to the 3′ end of PDC5 sadB-Fragment B-Fragment U, and primer oBP539 (SEQ ID NO: 1042). PCR products were purified with a PCR Purification kit (Qiagen). PDC5 sadB-Fragment B-Fragment U-Fragment C was created by overlapping PCR by mixing PDC5 sadB-Fragment B-Fragment U and PDC5 Fragment C and amplifying with primers oBP536 (SEQ ID NO: 1039) and oBP539 (SEQ ID NO: 1042). The resulting PCR product was purified on an agarose gel followed by a Gel Extraction kit (Qiagen). The PDC5 A-sadB-BUC cassette (SEQ ID NO: 1055) was created by amplifying PDC5 sadB-Fragment B-Fragment U-Fragment C with primers oBP542 (SEQ ID NO: 1043), containing a 5′ tail with homology to the 50 nucleotides immediately upstream of the native PDC5 coding sequence, and oBP539 (SEQ ID NO: 1042). The PCR product was purified with a PCR Purification kit (Qiagen). 
     Competent cells of CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm were made and transformed with the PDC5 A-sadB-BUC PCR cassette using a Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformation mixtures were plated on synthetic complete media lacking uracil supplemented with 1% ethanol (no glucose) at 30° C. Transformants with a pdc5 knockout sadB integration were screened for by PCR with primers oBP540 (SEQ ID NO: 1044) and oBP541 (SEQ ID NO: 1045) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The absence of the PDC5 gene from the isolate was demonstrated by a negative PCR result using primers specific for the coding sequence of PDC5, oBP552 (SEQ ID NO: 1046) and oBP553 (SEQ ID NO: 1047). A correct transformant was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm Δpdc5::sadB-URA3. 
     CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm Δpdc5::sadB-URA3 was grown overnight in YPE (1% ethanol) and plated on synthetic complete medium supplemented with ethanol (no glucose) and containing 5-fluoro-orotic acid (0.1%) at 30° C. to select for isolates that lost the URA3 marker. The deletion of PDC5, integration of sadB, and marker removal were confirmed by PCR with primers oBP540 (SEQ ID NO: 1044) and oBP541 (SEQ ID NO: 1045) using genomic DNA prepared with a Gentra Puregene Yeast/Bact kit (Qiagen). The correct isolate was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm Δpdc5::sadB and designated as BP913. 
     GPD2 Deletion 
     To delete the endogenous GPD2 coding region, a gpd2::loxP-URA3-loxP cassette (SEQ ID NO: 1057) was PCR-amplified using loxP-URA3-loxP PCR (SEQ ID NO: 1052) as template DNA. loxP-URA3-loxP contains the URA3 marker from (ATCC #77107) flanked by loxP recombinase sites. PCR was done using Phusion DNA polymerase and primers LA512 and LA513 (SEQ ID NOs: 1029 and 1030, respectively). The GPD2 portion of each primer was derived from the 5′ region upstream of the GPD2 coding region and 3′ region downstream of the coding region such that integration of the loxP-URA3-loxP marker resulted in replacement of the GPD2 coding region. The PCR product was transformed into BP913 and transformants were selected on synthetic complete media lacking uracil supplemented with 1% ethanol (no glucose). Transformants were screened to verify correct integration by PCR using primers oBP582 and AA270 (SEQ ID NOs: 1048 and 1049, respectively). 
     The URA3 marker was recycled by transformation with pRS423::P GAL1 -cre (SEQ ID NO: 1011) and plating on synthetic complete media lacking histidine supplemented with 1% ethanol at 30° C. Transformants were streaked on synthetic complete medium supplemented with 1% ethanol and containing 5-fluoro-orotic acid (0.1%) and incubated at 30 C to select for isolates that lost the URA3 marker. 5-FOA resistant isolates were grown in YPE (1% ethanol) for removal of the pRS423::P GAL1 -cre plasmid. The deletion and marker removal were confirmed by PCR with primers oBP582 (SEQ ID NO: 1048) and oBP591 (SEQ ID NO: 1050). The correct isolate was selected as strain CEN.PK 113-7D Δura3::loxP Δhis3 Δpdc6 Δpdc1::ilvDSm Δpdc5::sadB Δgpd2::loxP and designated as BP1064. 
     Example 14 
     Shake Flask Experiment to Measure 2,3-dihydroxyisovalerate Accumulation and Isobutanol Production 
     The purpose of this Example was to show the effect on accumulation of the isobutanol pathway intermediate 2,3-dihydroxyisovalerate (DHIV) and show isobutanol production in isobutanologen strains with an integrated copy of the AFT1-L99A gene or a FRA2 deletion compared to the parent strain. Strains were transformed with isobutanol pathway plasmids pYZ090 (SEQ ID NO: 984; described in U.S. patent application Ser. No. 61/368,436, incorporated by reference herein) and pLH468 (SEQ ID NO: 985; described in U.S. application Ser. No. 61/246,844, incorporated by reference herein). These plasmids are also described briefly, as follows. 
     pYZ090 (SEQ ID NO: 984) was constructed to contain a chimeric gene having the coding region of the alsS gene from  Bacillus subtilis  (nt position 457-2172) expressed from the yeast CUP1 promoter (nt 2-449) and followed by the CYC1 terminator (nt 2181-2430) for expression of ALS, and a chimeric gene having the coding region of the ilvC gene from  Lactococcus lactis  (nt 3634-4656) expressed from the yeast ILV5 promoter (2433-3626) and followed by the ILV5 terminator (nt 4682-5304) for expression of KARI. 
     pLH468 (SEQ ID NO: 985) was constructed to contain: a chimeric gene having the coding region of the ilvD gene from  Streptococcus mutans  (nt position 3313-4849) expressed from the  S. cerevisiae  FBA1 promoter (nt 2109-3105) followed by the FBA1 terminator (nt 4858-5857) for expression of DHAD; a chimeric gene having the coding region of codon optimized horse liver alcohol dehydrogenase (nt 6286-7413) expressed from the  S. cerevisiae  GPM1 promoter (nt 7425-8181) followed by the ADH1 terminator (nt 5962-6277) for expression of ADH; and a chimeric gene having the coding region of the codon-optimized kivD gene from  Lactococcus lactis  (nt 9249-10895) expressed from the TDH3 promoter (nt 10896-11918) followed by the TDH3 terminator (nt 8237-9235) for expression of KivD. 
     A transformant of PNY1503 (parent strain) was designated PNY1504. A transformant of PNY1505 (fra2 deletion strain) was designated PNY1506. Transformants of PNY1541 and PNY1542 (AFT1-L99A integration strains) were designated PNY1543 and PNY1544, for PNY1541, and PNY1545 and PNY1546, for PNY1542. 
     Strains were grown in synthetic medium (Yeast Nitrogen Base without Amino Acids (Sigma-Aldrich, St. Louis, Mo.) and Yeast Synthetic Drop-Out Media Supplement without uracil and histidine (Clontech, Mountain View, Calif.)) supplemented with 100 mM MES pH5.5, 0.2% glucose, and 0.2% ethanol. Overnight cultures were grown in 15 mL of medium in 125 mL vented Erlenmeyer flasks at 30° C., 225 RPM in a New Brunswick Scientific I24 shaker. 18 ml of medium in 125 mL tightly-capped Erlenmeyer flasks was inoculated with overnight culture to an OD 600  0.5 and grown for six hours at 30° C., 225 RPM in a New Brunswick Scientific 124 shaker. After six hours, glucose was added to 2.5%, yeast extract was added to 5 g/L, and peptone was added to 10 g/L (time 0 hours). After 24 and 48 hours, culture supernatants (collected using Spin-X centrifuge tube filter units, Costar Cat. No. 8169) were analyzed by HPLC (method described in U.S. Patent Appl. Pub. No. US 2007/0092957, incorporated by reference herein) and LC/MS. Glucose and isobutanol concentrations were determined by HPLC. DHIV was separated and quantified by LC/MS on a Waters (Milford, Mass.) AcquityTQD system, using an Atlantis T3 (part #186003539) column. The column was maintained at 30° C. and the flow rate was 0.5 mL/min. The A mobile phase was 0.1% formic acid in water, and the B mobile phase was 0.1% formic acid in acetonitrile. Each run consisted of 1 min at 99% A, a linear gradient over 1 min to 25% B, followed by 1 min at 99% A. The column effluent was monitored for peaks at m/z=133 (negative ESI), with cone voltage 32.5V, by Waters ACQ_TQD (s/n QBA688) mass spectometry detector. DHIV typically emerged at 1.2 min. Baseline separation was obtained and peak areas for DHIV were converted to μM DHIV concentrations by reference to analyses of standards solutions made from a 1 M aqueous stock. 
     Table 18 shows the DHIV molar yield (moles of DHIV per moles of glucose consumed) and isobutanol titer of the AFT1-L99A strains (PNY1543, PNY1544, PNY1545, and PNY1546) and the FRA2 deletion strain (PNY1506) compared to the parent strain background (PNY1504) at 24 and 48 hours. AFT1-L99A expression or the FRA2 deletion both led to approximately a 50% decrease in the accumulation of DHIV. 
     
       
         
           
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                 DHIV molar yield and isobutanol titer. 
               
            
           
           
               
               
               
               
               
            
               
                   
                 24 Hr 
                 48 Hr 
                 24 Hr 
                 48 Hr 
               
               
                   
                 DHIV Yield 
                 DHIV Yield 
                 Isobutanol 
                 Isobutanol 
               
               
                 Strain 
                 (mol/mol) 
                 (mol/mol) 
                 Titer (g/L) 
                 Titer (g/L) 
               
               
                   
               
               
                 PNY1504 
                 0.044 
                 0.035 
                 3.7 
                 4.2 
               
               
                 PNY1543- 
                 0.017 
                 0.015 
                 4.1 
                 5.8 
               
               
                 PNY1544 
               
               
                 PNY1545- 
                 0.019 
                 0.018 
                 4.6 
                 5.5 
               
               
                 PNY1546 
               
               
                 PNY1506 
                 0.022 
                 0.020 
                 3.8 
                 4.7 
               
               
                   
               
            
           
         
       
     
     Data are the average of two independent flasks, for PNY1504 and PNY1506, and two independent transformants for the AFT1-L99A strains (PNY1543-PNY1544 and PNY1545-PNY1546). 
     The foregoing description of the specific embodiments will so fully reveal the general nature of the invention that others can, by applying knowledge within the skill of the art, readily modify and/or adapt for various applications such specific embodiments, without undue experimentation, without departing from the general concept of the present invention. Therefore, such adaptations and modifications are intended to be within the meaning and range of equivalents of the disclosed embodiments, based on the teaching and guidance presented herein. It is to be understood that the phraseology or terminology herein is for the purpose of description and not of limitation, such that the terminology or phraseology of the present specification is to be interpreted by the skilled artisan in light of the teachings and guidance. 
     
       
         
           
               
               
             
               
                 TABLE 12 
               
               
                   
               
             
            
               
                 HMMER2.0 [2.2g] 
                 Program name and version 
               
               
                 NAME dhad_for_hmm 
                 Name of the input sequence alignment file 
               
               
                 LENG 564 
                 Length of the alignment: include indels 
               
               
                 ALPH Amino 
                 Type of residues 
               
               
                 MAP yes 
                 Map of the match states to the columns of the alignment 
               
               
                 COM/app/public/hmmer/current/bin/hmmbuild -F dhad-exp_hmm dhad_for_hmm.aln 
                 Commands used to generate the file: this one means that hmmbuild (default patrameters) was applied to the alignment file 
               
               
                 COM/app/public/hmmer/current/bin/hmmcalibrate dhad-exp_hmm 
                 Commands used to generate the file: this one means that hmmcalibrate (default parametrs) was applied to the hmm profile 
               
               
                 NSEQ 8 
                 Number of sequences in the alignment file 
               
               
                 DATE Tue Jun 3 10:48:24 2008 
                 When was the file generated 
               
               
                 XT −8455 −4 −1000 −1000 −8455 −4 −8455 −4 
               
               
                 NULT −4 −8455 
                 The transition probability distribution for the null model (single G state). 
               
               
                 NULE 595 −1558 85 338 −294 453 −1158 197 249 902 −1085 −142 −21 −313 45 531 201 
                 The symbol emission probability distribution for the null model (G state); consists of K (e.g. 4 or 20) integers. The null 
               
               
                 384 −1998 −644 
                 probability used to convert these back to model probabilities is 1/K. 
               
               
                 EVD −499.650970 0.086142 
                 The extreme value distribution parameters μ and lambda respectively; both floating point values. Lambda is positive and 
               
               
                   
                 nonzero. These values are set when the model is calibrated with hmmcalibrate. 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 A 
                 C 
                 D 
                 E 
                 F 
                 G 
                 H 
                 I 
                 K 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 Position in 
               
               
                 HMM 
                 m-&gt;m 
                 m-&gt;i 
                 m-&gt;d 
                 i-&gt;m 
                 i-&gt;i 
                 d-&gt;m 
                 d-&gt;d 
                 b-&gt;m 
                 m-&gt;e 
                 L 
                 M 
                 N 
                 P 
                 Q 
                 R 
                 S 
                 T 
                 V 
                 W 
                 Y 
                 alignment 
               
               
                   
               
               
                   
                 −538 
                 * 
                 −1684 
               
               
                  1(M) 
                 −233 
                 −1296 
                 99 
                 1223 
                 −1477 
                 −1132 
                 89 
                 −1122 
                 420 
                 −1248 
                 1757 
                 1553 
                 −1296 
                 464 
                 −24 
                 −190 
                 −188 
                 −838 
                 −1578 
                 −985 
                 6 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −29 
                 −6203 
                 −7245 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 −538 
                 * 
               
               
                  2(E) 
                 −220 
                 −1288 
                 232 
                 1356 
                 −1807 
                 1016 
                 −70 
                 −1474 
                 190 
                 −1584 
                 −775 
                 132 
                 −1298 
                 300 
                 −282 
                 −183 
                 1140 
                 −1092 
                 −1872 
                 −1262 
                 7 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −29 
                 −6203 
                 −7245 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  3(K) 
                 −448 
                 −1932 
                 1558 
                 658 
                 −2220 
                 −1048 
                 40 
                 −1983 
                 1569 
                 −1938 
                 −1091 
                 1558 
                 −1319 
                 450 
                 −193 
                 −278 
                 −419 
                 −1552 
                 −2121 
                 −1397 
                 8 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −29 
                 −6203 
                 −7245 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  4(V) 
                 −404 
                 −498 
                 −1497 
                 −939 
                 −588 
                 −1810 
                 −640 
                 1591 
                 914 
                 −127 
                 335 
                 −962 
                 −1866 
                 −562 
                 −767 
                 −868 
                 −357 
                 1720 
                 −1169 
                 −763 
                 9 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −29 
                 −6203 
                 −7245 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  5(E) 
                 −265 
                 −1340 
                 −52 
                 1376 
                 −1572 
                 −1189 
                 113 
                 −1125 
                 1345 
                 −1287 
                 −496 
                 99 
                 −1321 
                 505 
                 198 
                 −218 
                 −205 
                 597 
                 −1598 
                 −1032 
                 10 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −29 
                 −6203 
                 −7245 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  6(S) 
                 256 
                 −397 
                 −1014 
                 −830 
                 −1841 
                 −646 
                 −862 
                 −1443 
                 −767 
                 −1740 
                 −963 
                 −568 
                 −1249 
                 −651 
                 −1007 
                 2267 
                 1586 
                 −862 
                 −2080 
                 −1672 
                 11 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −29 
                 −6203 
                 −7245 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  7(M) 
                 −990 
                 −889 
                 −2630 
                 157 
                 −513 
                 −2514 
                 −1346 
                 1309 
                 −1767 
                 820 
                 3683 
                 −1898 
                 −2491 
                 −1496 
                 −1799 
                 −1589 
                 −925 
                 150 
                 −1336 
                 −1041 
                 12 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  8(E) 
                 588 
                 −1875 
                 −194 
                 1536 
                 −2188 
                 −1373 
                 −59 
                 −1931 
                 957 
                 −1890 
                 −977 
                 904 
                 292 
                 393 
                 −162 
                 483 
                 −372 
                 −1495 
                 −2070 
                 −1391 
                 13 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  9(N) 
                 −514 
                 −1116 
                 1207 
                 −315 
                 447 
                 −1650 
                 −304 
                 −778 
                 −224 
                 825 
                 −277 
                 1457 
                 −1738 
                 −123 
                 −618 
                 −627 
                 −454 
                 −603 
                 −1186 
                 763 
                 14 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  10(N) 
                 −815 
                 −1190 
                 −1360 
                 −922 
                 −904 
                 −1967 
                 −797 
                 −442 
                 −670 
                 381 
                 1700 
                 3009 
                 −2099 
                 −654 
                 −934 
                 −1051 
                 −791 
                 −445 
                 −1490 
                 −979 
                 15 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  11(K) 
                 −1530 
                 −2498 
                 −1722 
                 −855 
                 −3141 
                 −2246 
                 −428 
                 −2627 
                 2828 
                 −2404 
                 −1656 
                 −927 
                 662 
                 −2 
                 2047 
                 −1421 
                 −1337 
                 −2324 
                 −2357 
                 −2081 
                 16 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  12(Y) 
                 −872 
                 −1887 
                 −861 
                 −290 
                 −1369 
                 −1801 
                 1662 
                 −1797 
                 325 
                 −1793 
                 −1031 
                 893 
                 −1876 
                 56 
                 2219 
                 −812 
                 −780 
                 −1514 
                 −1565 
                 2287 
                 17 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  13(S) 
                 −830 
                 −1586 
                 −1471 
                 −1099 
                 −2717 
                 −1642 
                 −1010 
                 −2479 
                 −266 
                 −2518 
                 −1746 
                 −1065 
                 −2069 
                 −676 
                 1822 
                 2748 
                 −1000 
                 −1950 
                 −2597 
                 −2189 
                 18 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  14(Q) 
                 −851 
                 −2131 
                 −775 
                 −153 
                 −2554 
                 −1735 
                 −211 
                 −2205 
                 1908 
                 −2094 
                 −1244 
                 −386 
                 −1802 
                 2254 
                 974 
                 1001 
                 −747 
                 −1819 
                 −2181 
                 −1667 
                 19 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  15(T) 
                 −405 
                 −1258 
                 −618 
                 −100 
                 −1490 
                 −1466 
                 1158 
                 −1121 
                 1 
                 −1299 
                 −514 
                 578 
                 −1607 
                 65 
                 −433 
                 960 
                 1849 
                 343 
                 −1677 
                 −1143 
                 20 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  16(I) 
                 −1772 
                 −1325 
                 −4307 
                 −3877 
                 −1405 
                 −3993 
                 −3383 
                 2935 
                 −3705 
                 820 
                 −217 
                 −3632 
                 −3761 
                 −3400 
                 −3682 
                 −3260 
                 −1742 
                 2033 
                 −2838 
                 −2525 
                 21 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  17(T) 
                 −1018 
                 −1329 
                 −2004 
                 −1771 
                 −409 
                 −1993 
                 −1000 
                 −1256 
                 −1512 
                 −1464 
                 −966 
                 −1543 
                 −2367 
                 −1428 
                 −1638 
                 −1257 
                 3050 
                 −1090 
                 −1012 
                 2448 
                 22 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  18(Q) 
                 −1509 
                 −3056 
                 1970 
                 44 
                 −3310 
                 −1666 
                 −896 
                 −3242 
                 −877 
                 −3158 
                 −2439 
                 −322 
                 −2123 
                 3562 
                 −1493 
                 −1259 
                 −1550 
                 −2779 
                 −3260 
                 −2446 
                 23 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  19(D) 
                 −1006 
                 −2199 
                 2178 
                 −88 
                 −3159 
                 1997 
                 −936 
                 −2974 
                 −948 
                 −2977 
                 −2174 
                 −382 
                 −1960 
                 −589 
                 −1571 
                 1295 
                 −1157 
                 −2369 
                 −3178 
                 −2430 
                 24 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  20(M) 
                 445 
                 −796 
                 −1082 
                 −521 
                 −841 
                 −1643 
                 −412 
                 −403 
                 −370 
                 −692 
                 2213 
                 −646 
                 536 
                 1166 
                 −698 
                 −630 
                 660 
                 831 
                 −1204 
                 −767 
                 25 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  21(Q) 
                 741 
                 −990 
                 −1025 
                 −507 
                 −1249 
                 −1551 
                 −519 
                 −720 
                 −357 
                 −1062 
                 −345 
                 −635 
                 −1739 
                 1770 
                 −713 
                 −589 
                 1576 
                 1129 
                 −1559 
                 −1097 
                 26 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  22(R) 
                 −1753 
                 −2648 
                 −2072 
                 −1047 
                 −3365 
                 −2405 
                 −452 
                 −2782 
                 1989 
                 −2495 
                 −1773 
                 −1062 
                 −2379 
                 2402 
                 2643 
                 −1629 
                 −1506 
                 −2504 
                 −2397 
                 −2190 
                 27 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  23(S) 
                 −330 
                 −1010 
                 −1820 
                 −1628 
                 −2778 
                 −1229 
                 −1652 
                 −2481 
                 −1592 
                 −2691 
                 −1841 
                 −1273 
                 2130 
                 −1426 
                 −1834 
                 2449 
                 1034 
                 −1716 
                 −2961 
                 −2594 
                 28 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  24(P) 
                 1882 
                 −1119 
                 −2231 
                 −2302 
                 −3062 
                 −1360 
                 −2209 
                 −2710 
                 −2339 
                 −3013 
                 −2243 
                 −1676 
                 3304 
                 −2117 
                 −2409 
                 −742 
                 −918 
                 −1916 
                 −3263 
                 −3022 
                 29 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  25(N) 
                 969 
                 −1230 
                 −1066 
                 −915 
                 −2593 
                 −1313 
                 −1196 
                 −2242 
                 −1033 
                 −2447 
                 −1626 
                 3197 
                 −1850 
                 −898 
                 −1392 
                 −582 
                 1155 
                 −1644 
                 −2736 
                 −2256 
                 30 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  26(R) 
                 −1847 
                 −2640 
                 −2014 
                 −1161 
                 −3282 
                 −2428 
                 −579 
                 −2818 
                 687 
                 −2553 
                 −1869 
                 −1165 
                 −2462 
                 2447 
                 3181 
                 −1746 
                 −1630 
                 −2555 
                 −2447 
                 −2228 
                 31 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  27(A) 
                 3048 
                 −932 
                 −2480 
                 −2533 
                 −3075 
                 −1200 
                 −2274 
                 −2765 
                 −2501 
                 −3071 
                 −2221 
                 −1658 
                 −1948 
                 −2205 
                 −2512 
                 1225 
                 −739 
                 −1842 
                 −3322 
                 −3078 
                 32 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  28(M) 
                 −2406 
                 −2296 
                 −3638 
                 −3594 
                 −1525 
                 −3105 
                 −2824 
                 −1047 
                 −3121 
                 −596 
                 5043 
                 −3293 
                 −3425 
                 −3046 
                 −2996 
                 −2911 
                 −2552 
                 −1398 
                 −2513 
                 −2207 
                 33 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  29(Y) 
                 −1674 
                 −1506 
                 −2863 
                 −2464 
                 596 
                 −2872 
                 2251 
                 −972 
                 −2024 
                 2197 
                 −552 
                 −1986 
                 −2876 
                 −1739 
                 −1988 
                 −1987 
                 −1601 
                 −1002 
                 −95 
                 2332 
                 34 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  30(Y) 
                 −2013 
                 −2305 
                 −2428 
                 −1781 
                 −328 
                 −2709 
                 −654 
                 −2240 
                 −258 
                 −2064 
                 −1626 
                 −1631 
                 −2788 
                 −899 
                 2789 
                 −2017 
                 −1896 
                 −2130 
                 −857 
                 3434 
                 35 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  31(A) 
                 2822 
                 −1031 
                 −2418 
                 −2539 
                 −3226 
                 1898 
                 −2364 
                 −2941 
                 −2626 
                 −3229 
                 −2379 
                 −1722 
                 −2026 
                 −2302 
                 −2634 
                 −654 
                 −848 
                 −1983 
                 −3415 
                 −3226 
                 36 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  32(I) 
                 −1247 
                 −941 
                 −3569 
                 −3039 
                 −1082 
                 −3101 
                 −2185 
                 2227 
                 −2763 
                 766 
                 −76 
                 −2700 
                 −3050 
                 −2469 
                 −2697 
                 −2253 
                 1322 
                 1974 
                 −1988 
                 −1633 
                 37 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  33(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 38 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  34(F) 
                 −1511 
                 −1236 
                 −3511 
                 −3017 
                 2747 
                 −2982 
                 −1069 
                 −260 
                 −2651 
                 992 
                 2737 
                 −2407 
                 −2904 
                 −2088 
                 −2418 
                 −2099 
                 −1434 
                 −489 
                 −537 
                 2056 
                 39 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  35(Q) 
                 −576 
                 −1869 
                 −401 
                 92 
                 −2232 
                 831 
                 −173 
                 −1930 
                 1505 
                 −1913 
                 −1042 
                 −186 
                 −1620 
                 1653 
                 −51 
                 −482 
                 1346 
                 −1534 
                 −2098 
                 −1490 
                 40 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  36(D) 
                 −1352 
                 −3066 
                 3028 
                 1349 
                 −3303 
                 −1566 
                 −724 
                 −3141 
                 1155 
                 −3043 
                 −2267 
                 −165 
                 −1991 
                 −354 
                 −1350 
                 −1086 
                 −1368 
                 −2659 
                 −3221 
                 −2356 
                 41 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  37(E) 
                 −1507 
                 −3288 
                 2042 
                 2762 
                 −3520 
                 515 
                 −853 
                 −3401 
                 −981 
                 −3296 
                 −2566 
                 −182 
                 −2064 
                 −503 
                 −1753 
                 −1209 
                 −1553 
                 −2895 
                 −3486 
                 −2547 
                 42 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  38(D) 
                 −1445 
                 −2778 
                 3529 
                 −53 
                 −3524 
                 −1590 
                 −1129 
                 −3476 
                 −1367 
                 −3459 
                 −2774 
                 −396 
                 −2156 
                 −825 
                 −2122 
                 554 
                 −1609 
                 −2880 
                 −3582 
                 −2717 
                 43 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  39(F) 
                 −2658 
                 −2176 
                 −4213 
                 −4000 
                 3815 
                 −3933 
                 −1352 
                 −531 
                 −3638 
                 1121 
                 −19 
                 −3184 
                 −3709 
                 −2820 
                 −3296 
                 −3219 
                 −2579 
                 −1037 
                 −601 
                 403 
                 44 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  40(D) 
                 −684 
                 −2193 
                 1738 
                 1460 
                 −2494 
                 −1437 
                 −249 
                 −2257 
                 1694 
                 −2199 
                 −1308 
                 −62 
                 −1637 
                 185 
                 −450 
                 −531 
                 633 
                 −1808 
                 −2374 
                 −1657 
                 45 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  41(K) 
                 −2620 
                 −2961 
                 −2461 
                 −2046 
                 −3743 
                 −2791 
                 −1570 
                 −3603 
                 3784 
                 −3387 
                 −2839 
                 −2048 
                 −3039 
                 −1260 
                 −465 
                 −2604 
                 −2536 
                 −3331 
                 −3001 
                 −2988 
                 46 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  42(P) 
                 1882 
                 −1119 
                 −2231 
                 −2302 
                 −3062 
                 −1360 
                 −2209 
                 −2710 
                 −2339 
                 −3013 
                 −2243 
                 −1676 
                 3304 
                 −2117 
                 −2409 
                 −742 
                 −918 
                 −1916 
                 −3263 
                 −3022 
                 47 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  43(I) 
                 −1006 
                 −992 
                 −2347 
                 −1784 
                 −650 
                 −2452 
                 −1256 
                 2372 
                 −1386 
                 77 
                 2213 
                 −1720 
                 −2455 
                 2030 
                 −1490 
                 −1528 
                 −946 
                 106 
                 −1441 
                 −1111 
                 48 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  44(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 49 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  45(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 50 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  46(I) 
                 −1759 
                 −1303 
                 −4330 
                 −3968 
                 −1751 
                 −4051 
                 −3743 
                 3027 
                 −3837 
                 −597 
                 −528 
                 −3729 
                 −3875 
                 −3688 
                 −3910 
                 −3369 
                 −1751 
                 2438 
                 −3259 
                 −2819 
                 51 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  47(V) 
                 1736 
                 −1012 
                 −3546 
                 −3078 
                 −1377 
                 −3073 
                 −2434 
                 2052 
                 −2843 
                 −608 
                 −331 
                 −2754 
                 −3122 
                 −2619 
                 −2855 
                 −2270 
                 −1277 
                 2193 
                 −2333 
                 −1941 
                 52 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  48(N) 
                 −686 
                 −1511 
                 −702 
                 −806 
                 −2927 
                 −1386 
                 −1339 
                 −2841 
                 −1264 
                 −2950 
                 −2137 
                 2702 
                 −1979 
                 −1062 
                 −1648 
                 2444 
                 −971 
                 −2105 
                 −3054 
                 −2475 
                 53 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  49(M) 
                 −411 
                 −857 
                 −1800 
                 −1434 
                 −1528 
                 1914 
                 −1202 
                 −1029 
                 −1247 
                 −1347 
                 2989 
                 −1217 
                 −1912 
                 −1119 
                 −1444 
                 −676 
                 1550 
                 −767 
                 −1922 
                 −1539 
                 54 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  50(W) 
                 −782 
                 −1258 
                 793 
                 −683 
                 1193 
                 346 
                 2051 
                 −932 
                 −556 
                 −1092 
                 −441 
                 −798 
                 −1993 
                 −426 
                 −909 
                 −904 
                 −720 
                 −779 
                 3163 
                 1546 
                 55 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  51(W) 
                 1009 
                 −798 
                 −1470 
                 −935 
                 −463 
                 −1773 
                 −545 
                 −460 
                 −751 
                 −736 
                 −66 
                 −943 
                 −1904 
                 −606 
                 −1002 
                 1604 
                 −507 
                 −322 
                 2535 
                 1521 
                 56 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  52(D) 
                 −1137 
                 −2711 
                 2125 
                 1647 
                 −2995 
                 −1523 
                 −617 
                 −2786 
                 −528 
                 −2743 
                 −1933 
                 −150 
                 −1897 
                 −234 
                 −1165 
                 −924 
                 2117 
                 −2331 
                 −2948 
                 −2141 
                 57 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  53(I) 
                 −599 
                 −1102 
                 −1031 
                 −829 
                 −1522 
                 1429 
                 −927 
                 2119 
                 −880 
                 −1369 
                 −699 
                 1692 
                 −1938 
                 −759 
                 −1188 
                 −799 
                 −698 
                 −689 
                 −1887 
                 −1419 
                 58 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  54(T) 
                 −666 
                 −1412 
                 −954 
                 −984 
                 −2702 
                 −1428 
                 −1357 
                 −2418 
                 −1208 
                 −2650 
                 −1886 
                 2293 
                 −2000 
                 −1101 
                 −1519 
                 −787 
                 2967 
                 −1835 
                 −2866 
                 −2360 
                 59 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  55(P) 
                 −632 
                 −1230 
                 −2074 
                 −2144 
                 −2996 
                 −1453 
                 −2116 
                 −2631 
                 −2128 
                 −2928 
                 −2213 
                 −1658 
                 3610 
                 −2006 
                 −2221 
                 −852 
                 1302 
                 −1931 
                 −3185 
                 −2917 
                 60 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  56(C) 
                 −2476 
                 5735 
                 −4102 
                 −4358 
                 −3712 
                 −2763 
                 −3545 
                 −3518 
                 −4167 
                 −3859 
                 −3569 
                 −3631 
                 −3363 
                 −4030 
                 −3832 
                 −2793 
                 −2860 
                 −3158 
                 −3464 
                 −3718 
                 61 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  57(N) 
                 −2171 
                 −2655 
                 −1458 
                 −1748 
                 −3334 
                 −2364 
                 −2267 
                 −3943 
                 −2365 
                 −3936 
                 −3437 
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                 — 
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                 43 
                 −381 
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                 −626 
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                 45 
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                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                  58(M) 
                 672 
                 −918 
                 −3119 
                 −2578 
                 −742 
                 −2668 
                 −1734 
                 1807 
                 −2263 
                 16 
                 3713 
                 −2271 
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                 −1960 
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                 — 
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                 106 
                 −626 
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                 — 
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                 * 
                 * 
               
               
                  59(H) 
                 −1525 
                 −2164 
                 −1235 
                 −1346 
                 −2509 
                 2296 
                 4235 
                 −3172 
                 −1516 
                 −3178 
                 −2523 
                 −1448 
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                 — 
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                 −381 
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                 — 
                 −16 
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                 * 
                 * 
               
               
                  60(L) 
                 −2478 
                 −2009 
                 −4717 
                 −4196 
                 −568 
                 −4424 
                 −3262 
                 1334 
                 −3887 
                 2824 
                 604 
                 −4085 
                 −3872 
                 −3088 
                 −3590 
                 −3717 
                 −2380 
                 −199 
                 −2217 
                 −2207 
                 65 
               
               
                 — 
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                 106 
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                 45 
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                 — 
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                 −7108 
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                 * 
                 * 
               
               
                  61(H) 
                 −682 
                 −2191 
                 1015 
                 275 
                 −2485 
                 396 
                 2379 
                 −2251 
                 62 
                 −2197 
                 −1307 
                 1826 
                 −1636 
                 1527 
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                 — 
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                 43 
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                 106 
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                 394 
                 45 
                 96 
                 359 
                 117 
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                 — 
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                 −7108 
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                 −1115 
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                 * 
                 * 
               
               
                  62(D) 
                 −575 
                 −1920 
                 1979 
                 184 
                 −2299 
                 94 
                 −242 
                 −2029 
                 114 
                 −2023 
                 −1144 
                 −120 
                 −1608 
                 186 
                 1063 
                 −469 
                 1413 
                 −1605 
                 −2229 
                 −1561 
                 67 
               
               
                 — 
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                 43 
                 −381 
                 399 
                 106 
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                 45 
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                 — 
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                 * 
                 * 
               
               
                  63(L) 
                 −2618 
                 −2139 
                 −4597 
                 −4163 
                 2144 
                 −4285 
                 −2334 
                 −83 
                 −3854 
                 2690 
                 538 
                 −3771 
                 −3806 
                 −2950 
                 −3488 
                 −3563 
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                 68 
               
               
                 — 
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                 43 
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                 — 
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                 * 
                 * 
               
               
                  64(A) 
                 2657 
                 −1033 
                 −2408 
                 −2532 
                 −3233 
                 2193 
                 −2364 
                 −2950 
                 −2626 
                 −3237 
                 −2386 
                 −1719 
                 −2027 
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                 −2635 
                 −655 
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                 −3231 
                 69 
               
               
                 — 
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                 43 
                 −381 
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                 45 
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                 117 
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                 — 
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                 −701 
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                 * 
                 * 
               
               
                  65(K) 
                 −443 
                 −1857 
                 958 
                 270 
                 −2158 
                 −1393 
                 −66 
                 −1890 
                 1839 
                 −442 
                 −957 
                 −36 
                 −1499 
                 1204 
                 −132 
                 616 
                 −382 
                 −1469 
                 −2048 
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                 70 
               
               
                 — 
                 −149 
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                 43 
                 −381 
                 399 
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                 394 
                 45 
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                 — 
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                 −7108 
                 −8150 
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                 −701 
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                 * 
                 * 
               
               
                  66(C) 
                 605 
                 1553 
                 739 
                 −17 
                 −1374 
                 −1488 
                 −182 
                 260 
                 969 
                 −203 
                 −397 
                 −263 
                 −1573 
                 159 
                 691 
                 −426 
                 −331 
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                 −1567 
                 −1032 
                 71 
               
               
                 — 
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                 −500 
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                 43 
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                 106 
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                 45 
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                 — 
                 −16 
                 −7108 
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                 * 
                 * 
               
               
                  67(A) 
                 2327 
                 −956 
                 −3193 
                 −2728 
                 −1289 
                 −2677 
                 −2114 
                 1664 
                 −2485 
                 −601 
                 −288 
                 −2403 
                 −2839 
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                 −2523 
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                 1617 
                 −2143 
                 −1765 
                 72 
               
               
                 — 
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                 43 
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                 — 
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                 * 
                 * 
               
               
                  68(K) 
                 −532 
                 −1656 
                 −490 
                 1321 
                 −1891 
                 −1527 
                 −172 
                 −124 
                 2206 
                 −1591 
                 −782 
                 −223 
                 −1619 
                 237 
                 −106 
                 −482 
                 −464 
                 −98 
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                 −1326 
                 73 
               
               
                 — 
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                 — 
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                 * 
                 * 
               
               
                  69(H) 
                 384 
                 −1854 
                 936 
                 889 
                 −2165 
                 −1363 
                 1498 
                 −1909 
                 1111 
                 −1866 
                 −948 
                 1091 
                 −1464 
                 421 
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                 −284 
                 −342 
                 −69 
                 −2043 
                 −1364 
                 74 
               
               
                 — 
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                 −500 
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                 43 
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                 45 
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                 — 
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                 −7108 
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                 * 
                 * 
               
               
                  70(G) 
                 1823 
                 −932 
                 −2330 
                 −2313 
                 −3120 
                 2511 
                 −2158 
                 −2865 
                 −2331 
                 −3098 
                 −2209 
                 −1563 
                 −1912 
                 −2032 
                 −2419 
                 1138 
                 −706 
                 −1883 
                 −3328 
                 −3077 
                 75 
               
               
                 — 
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                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
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                 45 
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                 — 
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                 −7108 
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                 * 
                 * 
               
               
                  71(V) 
                 −1760 
                 −1333 
                 −4244 
                 −3789 
                 −1262 
                 −3902 
                 −3190 
                 1495 
                 −3588 
                 1270 
                 −96 
                 −3536 
                 −3677 
                 −3238 
                 −3534 
                 −3148 
                 −1725 
                 2865 
                 −2654 
                 −2373 
                 76 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 45 
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                 — 
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                 * 
                 * 
               
               
                  72(W) 
                 −1054 
                 −2172 
                 −1112 
                 −403 
                 −2566 
                 −1917 
                 −286 
                 −2196 
                 2516 
                 −2095 
                 −1292 
                 1183 
                 −1958 
                 140 
                 1333 
                 −959 
                 −922 
                 −1867 
                 2591 
                 −1720 
                 77 
               
               
                 — 
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                 233 
                 43 
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                 106 
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                 — 
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                 −701 
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                 * 
                 * 
               
               
                  73(D) 
                 611 
                 −1995 
                 1525 
                 937 
                 −2295 
                 −1400 
                 −148 
                 −2043 
                 211 
                 −2006 
                 −1106 
                 −37 
                 −1553 
                 1420 
                 −312 
                 −408 
                 1235 
                 −1609 
                 −2193 
                 −1499 
                 78 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
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                 399 
                 106 
                 −626 
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                 — 
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                 * 
                 * 
               
               
                  74(A) 
                 2716 
                 −902 
                 −2380 
                 −2205 
                 −2799 
                 −1197 
                 −1975 
                 −2459 
                 −2081 
                 −2736 
                 −1895 
                 −1520 
                 −1895 
                 −1844 
                 −2201 
                 1191 
                 1299 
                 −1669 
                 −3045 
                 −2758 
                 79 
               
               
                 — 
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                 43 
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                 399 
                 106 
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                 45 
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                 — 
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                 * 
                 * 
               
               
                  75(G) 
                 −1709 
                 −2833 
                 2424 
                 −409 
                 −3781 
                 2819 
                 −1457 
                 −3777 
                 −1728 
                 −3733 
                 −3076 
                 −739 
                 −2389 
                 −1180 
                 −2441 
                 −1557 
                 −1893 
                 −3158 
                 −3660 
                 −3038 
                 80 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
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                 −369 
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                 — 
                 −212 
                 −2909 
                 −8150 
                 −273 
                 −2534 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  76(A) 
                 2529 
                 −1119 
                 −2614 
                 −2330 
                 −1245 
                 −1983 
                 −1829 
                 −377 
                 −2042 
                 1435 
                 −341 
                 −1937 
                 −2411 
                 −1873 
                 −2088 
                 −1266 
                 −1059 
                 −397 
                 −2063 
                 −1713 
                 82 
               
               
                 — 
                 −149 
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                 233 
                 43 
                 −381 
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                 106 
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                 — 
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                 * 
                 * 
               
               
                  77(W) 
                 −472 
                 −361 
                 −2421 
                 −1812 
                 −298 
                 −1979 
                 −826 
                 1164 
                 −1486 
                 −143 
                 2485 
                 873 
                 −2028 
                 −1185 
                 −1426 
                 −1048 
                 −412 
                 1116 
                 2999 
                 −454 
                 83 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
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                 394 
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                 — 
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                 * 
                 * 
               
               
                  78(P) 
                 −1198 
                 −1737 
                 −2187 
                 −2394 
                 −3665 
                 2006 
                 −2550 
                 −3630 
                 −2743 
                 −3756 
                 −3008 
                 −2052 
                 3474 
                 −2495 
                 −2835 
                 −1401 
                 −1593 
                 −2736 
                 −3511 
                 −3519 
                 84 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
                 394 
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                 — 
                 −16 
                 −7108 
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                 * 
                 * 
               
               
                  79(Q) 
                 −999 
                 −1075 
                 −2106 
                 −1568 
                 −726 
                 −2370 
                 −1175 
                 83 
                 −1185 
                 1373 
                 218 
                 −1566 
                 −2400 
                 2445 
                 −1340 
                 −1445 
                 −946 
                 1441 
                 −1501 
                 −1146 
                 85 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
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                 — 
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                 * 
                 * 
               
               
                  80(Q) 
                 −885 
                 −779 
                 −2609 
                 −2018 
                 −481 
                 −2414 
                 −1253 
                 1645 
                 −1736 
                 799 
                 1924 
                 −1827 
                 −2405 
                 2262 
                 −1752 
                 −1484 
                 −821 
                 802 
                 −1240 
                 −935 
                 86 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
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                 45 
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                 — 
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                 −7108 
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                 * 
                 * 
               
               
                  81(F) 
                 −3342 
                 −2776 
                 −4026 
                 −4232 
                 4354 
                 −3545 
                 −1431 
                 −2315 
                 −4038 
                 −1801 
                 −1900 
                 −3299 
                 −3780 
                 −3350 
                 −3645 
                 −3490 
                 −3420 
                 −2566 
                 −739 
                 349 
                 87 
               
               
                 — 
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                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
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                 275 
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                 — 
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                 * 
                 * 
               
               
                  82(G) 
                 −998 
                 −2100 
                 −120 
                 −175 
                 −2567 
                 2528 
                 2174 
                 −2558 
                 −587 
                 −2583 
                 −1806 
                 1422 
                 −1966 
                 −461 
                 −1038 
                 −925 
                 −1088 
                 −2095 
                 −2657 
                 −1948 
                 88 
               
               
                 — 
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                 −500 
                 233 
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                 399 
                 106 
                 −626 
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                 394 
                 45 
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                 — 
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                 −7108 
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                 −701 
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                 * 
                 * 
               
               
                  83(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 89 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
                 394 
                 45 
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                 359 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  84(I) 
                 −1286 
                 −1279 
                 −2907 
                 −2683 
                 −1446 
                 −2549 
                 −2198 
                 3290 
                 −2407 
                 −726 
                 −534 
                 −2386 
                 1172 
                 −2299 
                 −2437 
                 −1895 
                 −1392 
                 283 
                 −2302 
                 −1913 
                 90 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
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                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
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                 * 
                 * 
               
               
                  85(T) 
                 −493 
                 −1105 
                 −2189 
                 −2267 
                 −3101 
                 1880 
                 −2196 
                 −2791 
                 −2334 
                 −3081 
                 −2269 
                 −1649 
                 −2058 
                 −2099 
                 −2410 
                 −719 
                 3135 
                 −1948 
                 −3282 
                 −3046 
                 91 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  86(V) 
                 −1750 
                 −1296 
                 −4319 
                 −3957 
                 −1765 
                 −4038 
                 −3733 
                 2364 
                 −3826 
                 −619 
                 −543 
                 −3716 
                 −3869 
                 −3685 
                 −3902 
                 −3354 
                 −1743 
                 3012 
                 −3265 
                 −2817 
                 92 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  87(S) 
                 923 
                 −962 
                 −2348 
                 −2422 
                 −3132 
                 −1207 
                 −2248 
                 −2850 
                 −2440 
                 −3140 
                 −2285 
                 −1624 
                 −1954 
                 −2158 
                 −2477 
                 3171 
                 −758 
                 −1896 
                 −3362 
                 −3103 
                 93 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  88(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 94 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  89(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 95 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  90(I) 
                 −1880 
                 −1493 
                 −4193 
                 −3724 
                 −953 
                 −3837 
                 −2980 
                 3251 
                 −3420 
                 257 
                 2372 
                 −3485 
                 −3608 
                 −3005 
                 −3310 
                 −3087 
                 −1840 
                 617 
                 −2373 
                 −2155 
                 96 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  91(S) 
                 2150 
                 −939 
                 −2407 
                 −2415 
                 −3075 
                 −1197 
                 −2205 
                 −2781 
                 −2384 
                 −3065 
                 −2205 
                 −1613 
                 −1936 
                 −2105 
                 −2436 
                 2652 
                 −729 
                 −1850 
                 −3306 
                 −3049 
                 97 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  92(M) 
                 −979 
                 −1455 
                 −1242 
                 −1122 
                 −1434 
                 −1860 
                 −1131 
                 −1171 
                 −974 
                 −1285 
                 4091 
                 2176 
                 −2226 
                 −1017 
                 −1187 
                 −1166 
                 −1086 
                 −1063 
                 −1929 
                 −1345 
                 98 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  93(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 99 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  94(T) 
                 −959 
                 −1691 
                 −1249 
                 −949 
                 −2563 
                 −1747 
                 −929 
                 −2093 
                 1282 
                 −2263 
                 −1554 
                 −995 
                 −2115 
                 −600 
                 −354 
                 −1037 
                 3152 
                 −1726 
                 −2494 
                 −2098 
                 100 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  95(E) 
                 −572 
                 −1860 
                 −208 
                 2213 
                 −2107 
                 −1461 
                 −191 
                 −1808 
                 199 
                 −116 
                 −983 
                 −127 
                 318 
                 1199 
                 −269 
                 −475 
                 −517 
                 −1448 
                 −2078 
                 −1441 
                 101 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  96(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 102 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  97(M) 
                 −2406 
                 −2296 
                 −3638 
                 −3594 
                 −1525 
                 −3105 
                 −2824 
                 −1047 
                 −3121 
                 −596 
                 5043 
                 −3293 
                 −3425 
                 −3046 
                 −2996 
                 −2911 
                 −2552 
                 −1398 
                 −2513 
                 −2207 
                 103 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  98(R) 
                 −2097 
                 −2786 
                 −2688 
                 −1415 
                 −3622 
                 −2625 
                 −555 
                 −2964 
                 2585 
                 −2627 
                 −1957 
                 −1318 
                 −2577 
                 −137 
                 3015 
                 −1979 
                 −1791 
                 −2732 
                 −2469 
                 −2363 
                 104 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                  99(Y) 
                 −3615 
                 −2706 
                 −4169 
                 −4413 
                 2626 
                 −4044 
                 −396 
                 −2535 
                 −3993 
                 −1939 
                 −1985 
                 −2747 
                 −3930 
                 −2852 
                 −3446 
                 −3296 
                 −3494 
                 −2686 
                 347 
                 4252 
                 105 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 100(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 106 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 101(L) 
                 −2871 
                 −2457 
                 −4231 
                 −4103 
                 −1033 
                 −3803 
                 −3165 
                 −541 
                 −3734 
                 3130 
                 −31 
                 −3935 
                 −3797 
                 −3286 
                 −3484 
                 −3713 
                 −2869 
                 −1136 
                 −2394 
                 −2220 
                 107 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 102(V) 
                 −1381 
                 −1065 
                 −3714 
                 −3252 
                 −1453 
                 −3300 
                 −2646 
                 1872 
                 −3023 
                 −615 
                 −373 
                 −2949 
                 −3287 
                 −2816 
                 −3039 
                 −2506 
                 1346 
                 2750 
                 −2489 
                 −2087 
                 108 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 103(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 109 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 104(R) 
                 −2957 
                 −3022 
                 −3318 
                 −2735 
                 −3796 
                 −2998 
                 −1968 
                 −3912 
                 −846 
                 −3631 
                 −3157 
                 −2611 
                 −3280 
                 −1724 
                 4056 
                 −3026 
                 −2913 
                 −3650 
                 −3096 
                 −3185 
                 110 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 105(E) 
                 −1719 
                 −3572 
                 2596 
                 2779 
                 −3767 
                 −1632 
                 −993 
                 −3700 
                 −1241 
                 −3578 
                 −2920 
                 −234 
                 −2167 
                 −666 
                 −2090 
                 −1380 
                 −1789 
                 −3182 
                 −3742 
                 −2756 
                 111 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 106(V) 
                 −1746 
                 −1296 
                 −4308 
                 −3946 
                 −1757 
                 −4020 
                 −3712 
                 2190 
                 −3811 
                 −614 
                 −539 
                 −3702 
                 −3858 
                 −3667 
                 −3884 
                 −3336 
                 −1740 
                 3098 
                 −3250 
                 −2803 
                 112 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 107(I) 
                 −2091 
                 −1746 
                 −3971 
                 −3840 
                 −1676 
                 −3532 
                 −3289 
                 3684 
                 −3581 
                 −659 
                 −693 
                 −3562 
                 −3674 
                 −3445 
                 −3521 
                 −3194 
                 −2146 
                 449 
                 −2877 
                 −2493 
                 113 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 108(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 114 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 109(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 115 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 110(S) 
                 −352 
                 2942 
                 −2955 
                 −2957 
                 −2876 
                 −1254 
                 −2382 
                 −2573 
                 −2692 
                 −2927 
                 −2128 
                 −1827 
                 −2001 
                 −2405 
                 −2607 
                 3103 
                 −778 
                 −1757 
                 −3171 
                 −2911 
                 116 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 111(I) 
                 −2091 
                 −1746 
                 −3971 
                 −3840 
                 −1676 
                 −3532 
                 −3289 
                 3684 
                 −3581 
                 −659 
                 −693 
                 −3562 
                 −3674 
                 −3445 
                 −3521 
                 −3194 
                 −2146 
                 449 
                 −2877 
                 −2493 
                 117 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 112(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 118 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 113(T) 
                 1556 
                 −936 
                 −2493 
                 −2457 
                 −2805 
                 −1256 
                 −2159 
                 −2210 
                 −2319 
                 −2681 
                 −1932 
                 −1656 
                 −1974 
                 −2089 
                 −2352 
                 −598 
                 3235 
                 −1547 
                 −3111 
                 −2847 
                 119 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 114(C) 
                 1784 
                 2119 
                 −2013 
                 −1532 
                 −1093 
                 −1580 
                 −1089 
                 −436 
                 −1322 
                 −937 
                 −273 
                 1093 
                 −1932 
                 −1127 
                 −1472 
                 −748 
                 −515 
                 1585 
                 −1536 
                 −1163 
                 120 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 115(M) 
                 1831 
                 2019 
                 −2596 
                 −2038 
                 −605 
                 −1979 
                 −1126 
                 244 
                 −1727 
                 −359 
                 2501 
                 −1655 
                 −2145 
                 −1435 
                 −1683 
                 −1106 
                 −557 
                 1087 
                 −1153 
                 −804 
                 121 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 116(Q) 
                 −987 
                 −2211 
                 −43 
                 −62 
                 −2833 
                 2229 
                 −691 
                 −2616 
                 −407 
                 −2604 
                 −1797 
                 1197 
                 −1917 
                 2260 
                 −858 
                 −880 
                 −1045 
                 −2139 
                 −2772 
                 −2099 
                 122 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 117(G) 
                 2313 
                 −1042 
                 −2391 
                 −2526 
                 −3250 
                 2601 
                 −2372 
                 −2972 
                 −2637 
                 −3257 
                 −2407 
                 −1721 
                 −2032 
                 −2310 
                 −2646 
                 −662 
                 −859 
                 −2003 
                 −3434 
                 −3247 
                 123 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 118(Q) 
                 −914 
                 −2350 
                 −48 
                 1661 
                 −2621 
                 −1571 
                 2504 
                 −2400 
                 68 
                 −2331 
                 −1486 
                 −201 
                 −1796 
                 2646 
                 −351 
                 −754 
                 −865 
                 −1984 
                 −2463 
                 −1787 
                 124 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 119(W) 
                 −517 
                 −1294 
                 −733 
                 −183 
                 −1062 
                 −1605 
                 −234 
                 −1037 
                 19 
                 −1207 
                 −456 
                 1435 
                 −1690 
                 33 
                 756 
                 411 
                 −454 
                 −819 
                 3340 
                 1286 
                 125 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 120(M) 
                 410 
                 −469 
                 −2417 
                 −1828 
                 −341 
                 −2041 
                 −897 
                 195 
                 −1513 
                 −156 
                 3130 
                 −1534 
                 −2102 
                 −1230 
                 −1484 
                 −1117 
                 −507 
                 954 
                 −894 
                 2253 
                 126 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 121(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 127 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 122(G) 
                 2142 
                 −930 
                 −2334 
                 −2298 
                 −3100 
                 2237 
                 −2139 
                 −2842 
                 −2302 
                 −3074 
                 −2187 
                 −1557 
                 −1909 
                 −2010 
                 −2397 
                 1136 
                 −701 
                 −1871 
                 −3308 
                 −3053 
                 128 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 123(V) 
                 −1514 
                 −1144 
                 −3950 
                 −3459 
                 1821 
                 −3487 
                 −2577 
                 2274 
                 −3208 
                 −209 
                 −87 
                 −3112 
                 −3362 
                 −2864 
                 −3118 
                 −2680 
                 −1476 
                 2426 
                 −2194 
                 −1786 
                 129 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 124(V) 
                 −1743 
                 −1294 
                 −4292 
                 −3873 
                 −1511 
                 −3988 
                 −3433 
                 2287 
                 −3712 
                 598 
                 −319 
                 −3626 
                 −3774 
                 −3456 
                 −3716 
                 −3260 
                 −1717 
                 2790 
                 −2931 
                 −2577 
                 130 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 125(A) 
                 2911 
                 −954 
                 −2808 
                 −2665 
                 −2115 
                 −1577 
                 −2196 
                 −575 
                 −2445 
                 −1646 
                 −1202 
                 −1906 
                 −2208 
                 −2218 
                 −2451 
                 −901 
                 −876 
                 1294 
                 −2727 
                 −2394 
                 131 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 126(I) 
                 −1764 
                 −1323 
                 −4298 
                 −3936 
                 −1668 
                 −3994 
                 −3655 
                 3337 
                 −3783 
                 −508 
                 −462 
                 −3689 
                 −3838 
                 −3608 
                 −3835 
                 −3311 
                 −1759 
                 1847 
                 −3164 
                 −2747 
                 132 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 127(G) 
                 −1157 
                 −1705 
                 −2169 
                 −2375 
                 −3654 
                 3021 
                 −2534 
                 −3611 
                 −2730 
                 −3741 
                 −2984 
                 −2024 
                 2418 
                 −2475 
                 −2826 
                 −1361 
                 −1555 
                 −2705 
                 −3513 
                 −3509 
                 133 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 128(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 134 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 129(C) 
                 −2476 
                 5735 
                 −4102 
                 −4358 
                 −3712 
                 −2763 
                 −3545 
                 −3518 
                 −4167 
                 −3859 
                 −3569 
                 −3631 
                 −3363 
                 −4030 
                 −3832 
                 −2793 
                 −2860 
                 −3158 
                 −3464 
                 −3718 
                 135 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 130(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 136 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 131(K) 
                 −2620 
                 −2961 
                 −2461 
                 −2046 
                 −3743 
                 −2791 
                 −1570 
                 −3603 
                 3784 
                 −3387 
                 −2839 
                 −2048 
                 −3039 
                 −1260 
                 −465 
                 −2604 
                 −2536 
                 −3331 
                 −3001 
                 −2988 
                 137 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 132(N) 
                 −2171 
                 −2655 
                 −1458 
                 −1748 
                 −3334 
                 −2364 
                 −2267 
                 −3943 
                 −2365 
                 −3936 
                 −3437 
                 4205 
                 −2932 
                 −2205 
                 −2608 
                 −2224 
                 −2439 
                 −3392 
                 −3253 
                 −2909 
                 138 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 133(M) 
                 −2406 
                 −2296 
                 −3638 
                 −3594 
                 −1525 
                 −3105 
                 −2824 
                 −1047 
                 −3121 
                 −596 
                 5043 
                 −3293 
                 −3425 
                 −3046 
                 −2996 
                 −2911 
                 −2552 
                 −1398 
                 −2513 
                 −2207 
                 139 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 134(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 140 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 135(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 141 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 136(A) 
                 2180 
                 −935 
                 −2286 
                 −2196 
                 −3057 
                 1098 
                 −2058 
                 −2796 
                 −2174 
                 −3021 
                 −2134 
                 −1516 
                 −1898 
                 −1906 
                 −2302 
                 2146 
                 −689 
                 −1849 
                 −3256 
                 −2983 
                 142 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 137(M) 
                 −1799 
                 −1433 
                 −4142 
                 −3579 
                 −669 
                 −3668 
                 −2608 
                 1558 
                 −3293 
                 1235 
                 3799 
                 −3296 
                 −3401 
                 −2717 
                 −3088 
                 −2843 
                 −1726 
                 1156 
                 −2002 
                 −1868 
                 143 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 138(I) 
                 −2091 
                 −1746 
                 −3971 
                 −3840 
                 −1676 
                 −3532 
                 −3289 
                 3684 
                 −3581 
                 −659 
                 −693 
                 −3562 
                 −3674 
                 −3445 
                 −3521 
                 −3194 
                 −2146 
                 449 
                 −2877 
                 −2493 
                 144 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 139(A) 
                 3103 
                 −1036 
                 −2445 
                 −2572 
                 −3222 
                 1051 
                 −2380 
                 −2930 
                 −2650 
                 −3226 
                 −2381 
                 −1739 
                 −2034 
                 −2327 
                 −2648 
                 −664 
                 −857 
                 −1981 
                 −3412 
                 −3228 
                 145 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 140(M) 
                 −2325 
                 −1891 
                 −4598 
                 −4012 
                 −498 
                 −4222 
                 −3013 
                 1242 
                 −3722 
                 1864 
                 3929 
                 −3855 
                 −3711 
                 −2910 
                 −3414 
                 −3439 
                 −2215 
                 −299 
                 −2076 
                 −2098 
                 146 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 141(A) 
                 3103 
                 −1036 
                 −2445 
                 −2572 
                 −3222 
                 1051 
                 −2380 
                 −2930 
                 −2650 
                 −3226 
                 −2381 
                 −1739 
                 −2034 
                 −2327 
                 −2648 
                 −664 
                 −857 
                 −1981 
                 −3412 
                 −3228 
                 147 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 142(R) 
                 −1588 
                 −2442 
                 −1399 
                 −953 
                 −3069 
                 −2171 
                 −708 
                 −2795 
                 373 
                 −2625 
                 −1916 
                 1858 
                 −2357 
                 −324 
                 3294 
                 −1520 
                 −1505 
                 −2453 
                 −2523 
                 −2186 
                 148 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 143(M) 
                 −1448 
                 −1256 
                 −3396 
                 −2819 
                 −474 
                 −3024 
                 −1923 
                 175 
                 −2473 
                 2225 
                 2756 
                 −2574 
                 −2922 
                 −2063 
                 −2375 
                 −2153 
                 952 
                 −151 
                 −1599 
                 −1410 
                 149 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 144(N) 
                 −1662 
                 −3306 
                 2055 
                 78 
                 −3621 
                 −1643 
                 −1040 
                 −3622 
                 −1272 
                 −3531 
                 −2870 
                 3477 
                 −2182 
                 −724 
                 −2071 
                 −1371 
                 −1757 
                 −3092 
                 −3633 
                 −2700 
                 150 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 145(I) 
                 −1066 
                 −921 
                 −2828 
                 −2239 
                 −1041 
                 −2675 
                 −1601 
                 2235 
                 −1668 
                 −455 
                 −92 
                 −2067 
                 −2692 
                 −1688 
                 1701 
                 −1795 
                 −1024 
                 1960 
                 −1771 
                 −1396 
                 151 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 146(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 152 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 147(S) 
                 1568 
                 −940 
                 −2267 
                 −2192 
                 −3082 
                 1101 
                 −2068 
                 −2826 
                 −2185 
                 −3049 
                 −2159 
                 −1515 
                 −1901 
                 −1915 
                 −2313 
                 2603 
                 −694 
                 −1866 
                 −3279 
                 −3006 
                 153 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 148(I) 
                 −1880 
                 −1492 
                 −4195 
                 −3728 
                 −963 
                 −3841 
                 −2991 
                 3272 
                 −3425 
                 246 
                 2277 
                 −3490 
                 −3613 
                 −3014 
                 −3317 
                 −3092 
                 −1841 
                 628 
                 −2385 
                 −2163 
                 154 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 149(F) 
                 −2204 
                 −1797 
                 −3724 
                 −3473 
                 3206 
                 −3383 
                 −628 
                 −1077 
                 −3092 
                 −746 
                 3167 
                 −2502 
                 −3309 
                 −2372 
                 −2792 
                 −2535 
                 −2120 
                 −1245 
                 28 
                 2460 
                 155 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 150(V) 
                 1265 
                 −1028 
                 −3200 
                 −2994 
                 −1833 
                 −2150 
                 −2480 
                 417 
                 −2771 
                 −1122 
                 −818 
                 −2349 
                 −2640 
                 −2559 
                 −2766 
                 −1464 
                 −1118 
                 3028 
                 −2700 
                 −2325 
                 156 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 151(Y) 
                 −3482 
                 −2868 
                 −3701 
                 −3919 
                 238 
                 −3552 
                 −1112 
                 −3000 
                 −3638 
                 −2516 
                 −2526 
                 −3027 
                 −3772 
                 −3101 
                 −3341 
                 −3418 
                 −3527 
                 −3071 
                 −441 
                 4711 
                 157 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 152(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 158 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 153(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 159 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 154(T) 
                 −359 
                 −976 
                 −2225 
                 −2229 
                 −2900 
                 −1242 
                 −2074 
                 −2560 
                 −2170 
                 −2875 
                 −2064 
                 −1561 
                 −1958 
                 −1969 
                 −2247 
                 1110 
                 3375 
                 −1760 
                 −3152 
                 −2850 
                 160 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 155(I) 
                 −2091 
                 −1746 
                 −3971 
                 −3840 
                 −1676 
                 −3532 
                 −3289 
                 3684 
                 −3581 
                 −659 
                 −693 
                 −3562 
                 −3674 
                 −3445 
                 −3521 
                 −3194 
                 −2146 
                 449 
                 −2877 
                 −2493 
                 161 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 156(H) 
                 861 
                 −1924 
                 −384 
                 1010 
                 −2260 
                 −1477 
                 1787 
                 −1974 
                 1769 
                 −1918 
                 −1022 
                 −120 
                 −1566 
                 362 
                 697 
                 −417 
                 −459 
                 −1557 
                 −2073 
                 −1446 
                 162 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 157(P) 
                 −655 
                 −1502 
                 −711 
                 −557 
                 −2204 
                 −1463 
                 2143 
                 −2122 
                 −586 
                 −2233 
                 −1445 
                 −688 
                 2941 
                 −560 
                 −941 
                 855 
                 −805 
                 −1657 
                 −2369 
                 −1763 
                 163 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 158(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 164 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 159(H) 
                 −744 
                 −2193 
                 −114 
                 1118 
                 −2513 
                 −1512 
                 2486 
                 −2252 
                 1178 
                 −2183 
                 −1308 
                 2230 
                 −1689 
                 180 
                 −233 
                 −598 
                 −687 
                 −1823 
                 −2335 
                 −1670 
                 165 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 160(W) 
                 −2672 
                 −2139 
                 −3850 
                 −3748 
                 941 
                 −3611 
                 −469 
                 −1691 
                 −3306 
                 1047 
                 −1217 
                 −2551 
                 −3534 
                 −2514 
                 −2960 
                 −2788 
                 −2577 
                 −1799 
                 4205 
                 3466 
                 166 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 161(K) 
                 386 
                 −1981 
                 779 
                 279 
                 −2295 
                 −1403 
                 −114 
                 −2043 
                 2059 
                 −1991 
                 −1082 
                 941 
                 −1536 
                 1263 
                 −211 
                 −384 
                 −457 
                 −1602 
                 −2161 
                 −1476 
                 167 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 162(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 168 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 163(K) 
                 −1144 
                 −2365 
                 −912 
                 2048 
                 −2856 
                 −1912 
                 −326 
                 −2459 
                 2267 
                 −2295 
                 −1482 
                 −556 
                 −1989 
                 108 
                 1334 
                 −1013 
                 −1014 
                 −2093 
                 −2324 
                 −1881 
                 169 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 164(D) 
                 −1091 
                 −2610 
                 2941 
                 174 
                 −2957 
                 −1527 
                 −595 
                 −2750 
                 1084 
                 −2696 
                 −1877 
                 −176 
                 −1885 
                 −206 
                 −1006 
                 740 
                 −1098 
                 −2288 
                 −2880 
                 −2105 
                 170 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 165(L) 
                 −2387 
                 −1922 
                 −4674 
                 −4155 
                 −617 
                 −4366 
                 −3250 
                 1889 
                 −3865 
                 2650 
                 558 
                 −4023 
                 −3847 
                 −3098 
                 −3586 
                 −3647 
                 −2296 
                 −38 
                 −2247 
                 −2224 
                 171 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 166(N) 
                 −1021 
                 −2427 
                 1806 
                 133 
                 −2870 
                 −1499 
                 −635 
                 −2647 
                 −521 
                 −2640 
                 −1825 
                 2171 
                 −1874 
                 −255 
                 −1124 
                 −860 
                 2122 
                 −2184 
                 −2853 
                 −2090 
                 172 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 167(I) 
                 −1830 
                 −1390 
                 −4327 
                 −3873 
                 −1210 
                 −3994 
                 −3274 
                 2967 
                 −3678 
                 1259 
                 −30 
                 −3633 
                 −3730 
                 −3283 
                 −3604 
                 −3249 
                 −1791 
                 1570 
                 −2661 
                 −2417 
                 173 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 168(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 174 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 169(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 175 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 170(A) 
                 2440 
                 −824 
                 −2371 
                 −2082 
                 −1993 
                 −1344 
                 −1704 
                 −1264 
                 −1899 
                 −1832 
                 −1137 
                 −1517 
                 −1946 
                 −1674 
                 −2005 
                 1075 
                 −641 
                 1474 
                 −2390 
                 −2055 
                 176 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 171(F) 
                 −3342 
                 −2776 
                 −4026 
                 −4232 
                 4354 
                 −3545 
                 −1431 
                 −2315 
                 −4038 
                 −1801 
                 −1900 
                 −3299 
                 −3780 
                 −3350 
                 −3645 
                 −3490 
                 −3420 
                 −2566 
                 −739 
                 349 
                 177 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 172(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 178 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 173(A) 
                 2966 
                 −1031 
                 −2429 
                 −2551 
                 −3222 
                 1544 
                 −2368 
                 −2934 
                 −2633 
                 −3225 
                 −2377 
                 −1727 
                 −2028 
                 −2309 
                 −2637 
                 −656 
                 −850 
                 −1980 
                 −3412 
                 −3224 
                 179 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 174(V) 
                 −1769 
                 −1342 
                 −4255 
                 −3793 
                 −1216 
                 −3901 
                 −3162 
                 1633 
                 −3589 
                 1486 
                 −51 
                 −3537 
                 −3667 
                 −3214 
                 −3518 
                 −3143 
                 −1731 
                 2692 
                 −2609 
                 −2345 
                 180 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 175(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 181 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 176(Q) 
                 −729 
                 −2116 
                 −413 
                 1096 
                 −2484 
                 −1587 
                 1599 
                 −2186 
                 1695 
                 −2094 
                 −1219 
                 −223 
                 −1698 
                 2418 
                 90 
                 −599 
                 −649 
                 −1770 
                 −2213 
                 −1615 
                 182 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 177(W) 
                 −1652 
                 −1707 
                 −2340 
                 −1879 
                 1996 
                 −2733 
                 2013 
                 −1398 
                 1758 
                 −1386 
                 −938 
                 −1641 
                 −2751 
                 −1364 
                 −1762 
                 −1780 
                 −1577 
                 −1325 
                 3577 
                 2136 
                 183 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 178(T) 
                 −421 
                 −753 
                 −1251 
                 −704 
                 −846 
                 −1670 
                 −535 
                 894 
                 −548 
                 −690 
                 −1 
                 1376 
                 −1791 
                 −421 
                 −846 
                 373 
                 1461 
                 858 
                 −1236 
                 −812 
                 184 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 179(H) 
                 1498 
                 −1593 
                 −504 
                 15 
                 −1895 
                 −1484 
                 2279 
                 −1559 
                 1119 
                 −1640 
                 −810 
                 −242 
                 −1611 
                 194 
                 −171 
                 −462 
                 815 
                 −1231 
                 −1914 
                 −1340 
                 185 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 180(G) 
                 −1515 
                 −2130 
                 −1298 
                 −1450 
                 −2658 
                 3285 
                 2212 
                 −3276 
                 −1691 
                 −3291 
                 −2638 
                 −1524 
                 −2562 
                 −1662 
                 −1925 
                 −1600 
                 −1764 
                 −2713 
                 −2804 
                 −2234 
                 186 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 181(K) 
                 −528 
                 −2010 
                 1346 
                 1082 
                 −2329 
                 −1408 
                 −118 
                 −2080 
                 1475 
                 −2018 
                 −1108 
                 1161 
                 −1543 
                 331 
                 1052 
                 −394 
                 −471 
                 −1632 
                 −2181 
                 −1494 
                 187 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 182(M) 
                 −1894 
                 −1521 
                 −4170 
                 −3679 
                 −840 
                 −3793 
                 −2866 
                 2827 
                 −3360 
                 375 
                 3445 
                 −3437 
                 −3555 
                 −2902 
                 −3223 
                 −3028 
                 −1846 
                 470 
                 −2249 
                 −2059 
                 188 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 183(T) 
                 −670 
                 −1758 
                 1731 
                 −141 
                 −2591 
                 −1399 
                 −691 
                 −2319 
                 −499 
                 −2384 
                 −1543 
                 −387 
                 −1786 
                 −316 
                 −1016 
                 1576 
                 2044 
                 −1811 
                 −2624 
                 −1981 
                 189 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 184(E) 
                 345 
                 −2074 
                 925 
                 1994 
                 −2378 
                 −1408 
                 −177 
                 −2135 
                 922 
                 −2084 
                 −1183 
                 −38 
                 641 
                 264 
                 −356 
                 −444 
                 −536 
                 −1690 
                 −2261 
                 −1556 
                 190 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 185(E) 
                 −1493 
                 −2900 
                 93 
                 3174 
                 −2903 
                 −1743 
                 1987 
                 −3042 
                 −646 
                 −2957 
                 −2238 
                 −411 
                 −2146 
                 −506 
                 −1121 
                 −1272 
                 −1503 
                 −2629 
                 −2905 
                 −2134 
                 191 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 186(D) 
                 −1293 
                 −2959 
                 2673 
                 2121 
                 −3219 
                 −1546 
                 −713 
                 −3043 
                 −707 
                 −2974 
                 −2191 
                 −158 
                 −1967 
                 −342 
                 −1394 
                 −1043 
                 701 
                 −2567 
                 −3172 
                 −2311 
                 192 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 187(F) 
                 −1137 
                 −905 
                 −3250 
                 −2707 
                 2365 
                 −2647 
                 −1016 
                 −34 
                 −2336 
                 1239 
                 267 
                 −2150 
                 −2626 
                 −1861 
                 −2133 
                 −1752 
                 −1069 
                 1461 
                 −599 
                 1844 
                 193 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 188(K) 
                 −479 
                 −1713 
                 −409 
                 1031 
                 −1925 
                 −1467 
                 1755 
                 −1650 
                 1844 
                 −349 
                 −827 
                 −140 
                 −1556 
                 319 
                 −75 
                 −403 
                 −411 
                 −1301 
                 −1900 
                 843 
                 194 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 189(G) 
                 433 
                 −2144 
                 52 
                 1047 
                 −2717 
                 2303 
                 −615 
                 −2467 
                 −442 
                 −2482 
                 −1655 
                 1123 
                 −1828 
                 −233 
                 −995 
                 −763 
                 −923 
                 −2000 
                 −2710 
                 −2005 
                 195 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 190(V) 
                 −1752 
                 −1320 
                 −4254 
                 −3806 
                 −1311 
                 −3916 
                 −3232 
                 1701 
                 −3614 
                 1188 
                 −140 
                 −3551 
                 −3693 
                 −3280 
                 −3568 
                 −3166 
                 −1718 
                 2833 
                 −2703 
                 −2409 
                 196 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 191(E) 
                 −1199 
                 −1750 
                 −734 
                 2668 
                 −1820 
                 −2038 
                 −1068 
                 1892 
                 −867 
                 −1273 
                 −897 
                 −922 
                 −2295 
                 −797 
                 −1238 
                 −1340 
                 −1197 
                 −426 
                 −2325 
                 −1789 
                 197 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 192(C) 
                 −1182 
                 3528 
                 −1398 
                 −620 
                 −2541 
                 −2038 
                 −358 
                 −2093 
                 1181 
                 −2037 
                 −1272 
                 −747 
                 −2070 
                 1553 
                 2213 
                 −1123 
                 −1038 
                 −1817 
                 −2142 
                 −1774 
                 198 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 193(N) 
                 −1478 
                 −2527 
                 −261 
                 −403 
                 −2011 
                 −1837 
                 2032 
                 −2925 
                 −735 
                 −2845 
                 −2195 
                 3635 
                 −2259 
                 −721 
                 −1085 
                 −1352 
                 −1546 
                 −2522 
                 −2307 
                 −1431 
                 199 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 194(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 200 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 195(C) 
                 −1220 
                 4911 
                 −3609 
                 −3314 
                 −1440 
                 −2525 
                 −2482 
                 1565 
                 −2922 
                 −706 
                 −544 
                 −2678 
                 −2896 
                 −2710 
                 −2836 
                 −1869 
                 −1375 
                 379 
                 −2371 
                 −1957 
                 201 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 196(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 202 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 197(G) 
                 −477 
                 −1115 
                 −1983 
                 −2189 
                 −3315 
                 3154 
                 −2272 
                 −3172 
                 −2506 
                 −3387 
                 −2522 
                 −1599 
                 −2042 
                 −2177 
                 −2583 
                 1217 
                 −905 
                 −2130 
                 −3477 
                 −3225 
                 203 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 198(A) 
                 1653 
                 −1347 
                 −705 
                 −249 
                 −1969 
                 −1385 
                 −477 
                 −1629 
                 −159 
                 −1759 
                 −935 
                 −434 
                 1285 
                 1404 
                 −586 
                 −450 
                 1019 
                 −1243 
                 −2070 
                 −1522 
                 204 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 199(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 205 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 200(S) 
                 1870 
                 −938 
                 −2270 
                 −2183 
                 −3068 
                 1488 
                 −2056 
                 −2810 
                 −2168 
                 −3032 
                 −2144 
                 −1511 
                 −1898 
                 −1901 
                 −2300 
                 2236 
                 −690 
                 −1857 
                 −3265 
                 −2990 
                 206 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 201(C) 
                 −2476 
                 5735 
                 −4102 
                 −4358 
                 −3712 
                 −2763 
                 −3545 
                 −3518 
                 −4167 
                 −3859 
                 −3569 
                 −3631 
                 −3363 
                 −4030 
                 −3832 
                 −2793 
                 −2860 
                 −3158 
                 −3464 
                 −3718 
                 207 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 202(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 208 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 203(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 209 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 204(M) 
                 −2406 
                 −2296 
                 −3638 
                 −3594 
                 −1525 
                 −3105 
                 −2824 
                 −1047 
                 −3121 
                 −596 
                 5043 
                 −3293 
                 −3425 
                 −3046 
                 −2996 
                 −2911 
                 −2552 
                 −1398 
                 −2513 
                 −2207 
                 210 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 205(Y) 
                 −3590 
                 −2700 
                 −4146 
                 −4379 
                 2092 
                 −4028 
                 −404 
                 −2517 
                 −3963 
                 −1928 
                 −1973 
                 −2744 
                 −3921 
                 −2845 
                 −3431 
                 −3284 
                 −3474 
                 −2669 
                 336 
                 4423 
                 211 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 206(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 212 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 207(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 213 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 208(N) 
                 −2171 
                 −2655 
                 −1458 
                 −1748 
                 −3334 
                 −2364 
                 −2267 
                 −3943 
                 −2365 
                 −3936 
                 −3437 
                 4205 
                 −2932 
                 −2205 
                 −2608 
                 −2224 
                 −2439 
                 −3392 
                 −3253 
                 −2909 
                 214 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 209(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 215 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 210(M) 
                 −2355 
                 −1988 
                 −4343 
                 −3834 
                 −504 
                 −4051 
                 −2868 
                 105 
                 −3385 
                 1451 
                 4460 
                 −3680 
                 −3671 
                 −2806 
                 −3171 
                 −3327 
                 −2274 
                 −474 
                 −2039 
                 −1925 
                 216 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 211(S) 
                 2150 
                 −939 
                 −2407 
                 −2415 
                 −3075 
                 −1197 
                 −2205 
                 −2781 
                 −2384 
                 −3065 
                 −2205 
                 −1613 
                 −1936 
                 −2105 
                 −2436 
                 2652 
                 −729 
                 −1850 
                 −3306 
                 −3049 
                 217 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 212(S) 
                 −344 
                 −979 
                 −2190 
                 −2162 
                 −2959 
                 −1227 
                 −2042 
                 −2651 
                 −2116 
                 −2934 
                 −2100 
                 −1526 
                 −1941 
                 −1909 
                 −2222 
                 2940 
                 1775 
                 −1804 
                 −3187 
                 −2882 
                 218 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 213(A) 
                 3048 
                 −932 
                 −2480 
                 −2533 
                 −3075 
                 −1200 
                 −2274 
                 −2765 
                 −2501 
                 −3071 
                 −2221 
                 −1658 
                 −1948 
                 −2205 
                 −2512 
                 1225 
                 −739 
                 −1842 
                 −3322 
                 −3078 
                 219 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 214(I) 
                 −1924 
                 −1546 
                 −4067 
                 −3658 
                 2312 
                 −3663 
                 −2081 
                 3030 
                 −3367 
                 150 
                 99 
                 −3197 
                 −3492 
                 −2821 
                 −3179 
                 −2894 
                 −1877 
                 293 
                 −1445 
                 −692 
                 220 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 215(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 221 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 216(A) 
                 2389 
                 −814 
                 −2506 
                 −2162 
                 −1696 
                 −1545 
                 −1698 
                 −499 
                 −1942 
                 −1398 
                 −813 
                 −1640 
                 −2076 
                 −1723 
                 −2027 
                 −806 
                 1148 
                 1559 
                 −2200 
                 −1856 
                 222 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 217(M) 
                 −2576 
                 −2118 
                 −4725 
                 −4165 
                 −461 
                 −4430 
                 −3165 
                 99 
                 −3811 
                 2513 
                 3454 
                 −4075 
                 −3839 
                 −2978 
                 −3488 
                 −3704 
                 −2457 
                 −591 
                 −2111 
                 −2145 
                 223 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 218(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 224 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 219(M) 
                 −2313 
                 −1968 
                 −4258 
                 −3765 
                 −518 
                 −3966 
                 −2806 
                 98 
                 −3289 
                 1292 
                 4523 
                 −3599 
                 −3636 
                 −2769 
                 −3097 
                 −3249 
                 −2243 
                 −457 
                 −2026 
                 −1874 
                 225 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 220(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 226 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 221(L) 
                 −2631 
                 −2159 
                 −4786 
                 −4228 
                 −462 
                 −4506 
                 −3231 
                 96 
                 −3878 
                 2828 
                 2482 
                 −4157 
                 −3880 
                 −3016 
                 −3541 
                 −3793 
                 −2509 
                 −608 
                 −2134 
                 −2182 
                 227 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 222(P) 
                 −1501 
                 −1778 
                 −2473 
                 −2371 
                 −1710 
                 −2311 
                 −2045 
                 −1321 
                 −2060 
                 827 
                 −1068 
                 −2173 
                 3594 
                 −2082 
                 −2130 
                 −1799 
                 −1699 
                 −1373 
                 −2373 
                 −1942 
                 228 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 223(Y) 
                 −1068 
                 −1670 
                 −865 
                 −836 
                 −631 
                 1198 
                 −767 
                 −1828 
                 −1059 
                 −1914 
                 −1304 
                 692 
                 −2203 
                 −906 
                 −1387 
                 −1136 
                 −1163 
                 −1566 
                 −1185 
                 3670 
                 229 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 224(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 230 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 225(S) 
                 1172 
                 −954 
                 −2367 
                 −2422 
                 −3120 
                 −1204 
                 −2237 
                 −2835 
                 −2426 
                 −3122 
                 −2265 
                 −1621 
                 −1948 
                 −2145 
                 −2467 
                 3107 
                 −749 
                 −1884 
                 −3349 
                 −3092 
                 231 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 226(S) 
                 −342 
                 −975 
                 −2176 
                 −2124 
                 −2912 
                 −1229 
                 −2003 
                 −2594 
                 −2067 
                 −2878 
                 −2048 
                 −1510 
                 −1936 
                 −1866 
                 −2184 
                 2553 
                 2492 
                 −1773 
                 −3143 
                 −2833 
                 232 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 227(M) 
                 −720 
                 −1440 
                 −710 
                 −343 
                 −1228 
                 −1693 
                 2436 
                 −1209 
                 −132 
                 −1364 
                 3099 
                 1904 
                 −1852 
                 −183 
                 −458 
                 −776 
                 −680 
                 −1004 
                 −1540 
                 −890 
                 233 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 228(P) 
                 2240 
                 −1100 
                 −2241 
                 −2293 
                 −3037 
                 −1346 
                 −2188 
                 −2683 
                 −2317 
                 −2986 
                 −2210 
                 −1663 
                 3041 
                 −2093 
                 −2391 
                 −722 
                 −895 
                 −1893 
                 −3243 
                 −2998 
                 234 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 229(A) 
                 2958 
                 −1235 
                 −1299 
                 −1377 
                 −2868 
                 −1345 
                 −1673 
                 −2580 
                 −1661 
                 −2843 
                 −2054 
                 1555 
                 −1995 
                 −1468 
                 −1921 
                 −715 
                 −888 
                 −1871 
                 −3064 
                 −2630 
                 235 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 230(E) 
                 −509 
                 −1046 
                 −884 
                 1564 
                 −1116 
                 −1669 
                 −441 
                 −485 
                 −283 
                 250 
                 −206 
                 −577 
                 689 
                 −200 
                 −656 
                 −670 
                 −459 
                 1290 
                 −1467 
                 −995 
                 236 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 231(D) 
                 −1203 
                 −2412 
                 2595 
                 −117 
                 −3286 
                 −1536 
                 −1057 
                 −3176 
                 −1165 
                 −3186 
                 −2436 
                 −428 
                 −2068 
                 −736 
                 −1824 
                 2377 
                 −1366 
                 −2578 
                 −3334 
                 −2552 
                 237 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 232(Q) 
                 954 
                 −1983 
                 −100 
                 971 
                 −2337 
                 177 
                 −267 
                 −2067 
                 81 
                 −2060 
                 −1189 
                 −125 
                 −1637 
                 2600 
                 −418 
                 −514 
                 −597 
                 −1649 
                 −2268 
                 −1597 
                 238 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 233(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 239 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 234(K) 
                 −2620 
                 −2961 
                 −2461 
                 −2046 
                 −3743 
                 −2791 
                 −1570 
                 −3603 
                 3784 
                 −3387 
                 −2839 
                 −2048 
                 −3039 
                 −1260 
                 −465 
                 −2604 
                 −2536 
                 −3331 
                 −3001 
                 −2988 
                 240 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 235(R) 
                 377 
                 −1802 
                 −415 
                 988 
                 −2095 
                 −1474 
                 −95 
                 −1786 
                 1452 
                 −1785 
                 −911 
                 −135 
                 −1560 
                 343 
                 1555 
                 −409 
                 −431 
                 376 
                 −1986 
                 −1375 
                 241 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 236(D) 
                 1083 
                 −1565 
                 2662 
                 −244 
                 −1941 
                 −1573 
                 −679 
                 612 
                 −527 
                 −1651 
                 −980 
                 −490 
                 −1869 
                 −358 
                 −1003 
                 −771 
                 −766 
                 −903 
                 −2208 
                 −1633 
                 242 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 237(E) 
                 −1225 
                 −2868 
                 1894 
                 1948 
                 −3149 
                 −1532 
                 −671 
                 −2975 
                 −630 
                 −2902 
                 −2101 
                 −150 
                 −1935 
                 −293 
                 −1299 
                 1884 
                 −1241 
                 −2496 
                 −3093 
                 −2248 
                 243 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 238(C) 
                 1375 
                 3262 
                 −2620 
                 −2108 
                 −827 
                 −1866 
                 −1267 
                 1631 
                 −1811 
                 −599 
                 −10 
                 −1674 
                 −2137 
                 −1531 
                 −1786 
                 −1034 
                 790 
                 249 
                 −1361 
                 −1010 
                 244 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 239(E) 
                 635 
                 −1796 
                 1055 
                 1761 
                 −2018 
                 −1464 
                 −263 
                 1191 
                 28 
                 −1767 
                 −946 
                 −148 
                 −1637 
                 135 
                 −481 
                 −520 
                 −553 
                 −1300 
                 −2077 
                 −1441 
                 245 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 240(E) 
                 593 
                 −2044 
                 −252 
                 2548 
                 −2437 
                 −1542 
                 −329 
                 −2133 
                 151 
                 −2120 
                 −1274 
                 −244 
                 −1738 
                 89 
                 946 
                 −646 
                 −717 
                 −1734 
                 −2305 
                 −1686 
                 246 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 241(S) 
                 1884 
                 −835 
                 −1962 
                 −1576 
                 −1634 
                 −1436 
                 −1320 
                 1041 
                 −1409 
                 −1453 
                 −781 
                 −1293 
                 −1922 
                 −1241 
                 −1606 
                 1973 
                 −597 
                 −669 
                 −2036 
                 −1656 
                 247 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 242(G) 
                 2267 
                 −1043 
                 −2388 
                 −2526 
                 −3253 
                 2642 
                 −2373 
                 −2975 
                 −2639 
                 −3260 
                 −2410 
                 −1722 
                 −2033 
                 −2311 
                 −2648 
                 −663 
                 −860 
                 −2005 
                 −3436 
                 −3250 
                 248 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 243(R) 
                 −876 
                 −2087 
                 −829 
                 1490 
                 −2474 
                 −1766 
                 −229 
                 −2106 
                 1269 
                 −44 
                 −1198 
                 −424 
                 −1829 
                 205 
                 2225 
                 −775 
                 −768 
                 −1753 
                 −2143 
                 −1647 
                 249 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 244(V) 
                 2339 
                 −967 
                 −2970 
                 −2766 
                 −1878 
                 −1847 
                 −2252 
                 32 
                 −2541 
                 −1299 
                 −918 
                 −2087 
                 −2399 
                 −2316 
                 −2545 
                 −1157 
                 −971 
                 2345 
                 −2605 
                 −2251 
                 250 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 245(I) 
                 −1827 
                 −1398 
                 −4307 
                 −3831 
                 −1099 
                 −3939 
                 −3142 
                 2286 
                 −3619 
                 1835 
                 69 
                 −3579 
                 −3671 
                 −3177 
                 −3511 
                 −3178 
                 −1781 
                 1918 
                 −2524 
                 −2310 
                 251 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 246(V) 
                 −1178 
                 −1448 
                 −1943 
                 −1452 
                 −1776 
                 −2261 
                 −1140 
                 −227 
                 1866 
                 −1260 
                 −816 
                 −1444 
                 −2448 
                 −902 
                 −540 
                 −1496 
                 −1176 
                 2697 
                 −2161 
                 −1764 
                 252 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 247(E) 
                 −508 
                 −1976 
                 840 
                 1547 
                 −2280 
                 −1393 
                 −117 
                 −2029 
                 1400 
                 −1984 
                 −1077 
                 1158 
                 −1531 
                 330 
                 −253 
                 −378 
                 −454 
                 262 
                 −2163 
                 −1471 
                 253 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 248(M) 
                 1703 
                 −991 
                 −2901 
                 −2342 
                 −528 
                 −2567 
                 −1550 
                 166 
                 −2031 
                 1544 
                 2668 
                 −2104 
                 −2591 
                 −1715 
                 −2010 
                 −1685 
                 −1052 
                 −12 
                 −1442 
                 −1177 
                 254 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 249(I) 
                 −1947 
                 −1516 
                 −4385 
                 −3885 
                 −916 
                 −4013 
                 −3118 
                 2193 
                 −3656 
                 2186 
                 257 
                 −3656 
                 −3687 
                 −3109 
                 −3494 
                 −3250 
                 −1889 
                 1383 
                 −2397 
                 −2258 
                 255 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 250(E) 
                 −1322 
                 −2647 
                 −272 
                 2491 
                 −3071 
                 −1811 
                 −576 
                 −2759 
                 2306 
                 −2633 
                 −1854 
                 −464 
                 −2066 
                 −175 
                 −177 
                 −1144 
                 −1256 
                 −2368 
                 −2692 
                 −2140 
                 256 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 251(K) 
                 −1395 
                 −2059 
                 −1711 
                 −1014 
                 −2215 
                 −2218 
                 −641 
                 −1709 
                 3021 
                 −1652 
                 2578 
                 −1075 
                 −2303 
                 −282 
                 287 
                 −1423 
                 −1283 
                 −1603 
                 −2159 
                 −1803 
                 257 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 252(D) 
                 −1285 
                 −2888 
                 2677 
                 176 
                 −3210 
                 1189 
                 −737 
                 −3047 
                 −715 
                 −2977 
                 −2195 
                 −190 
                 −1979 
                 2106 
                 −1379 
                 −1050 
                 −1315 
                 −2564 
                 −3161 
                 −2320 
                 258 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 253(I) 
                 −2073 
                 −1632 
                 −4434 
                 −3975 
                 −911 
                 −4130 
                 −3238 
                 3164 
                 −3706 
                 1451 
                 244 
                 −3779 
                 −3785 
                 −3187 
                 −3557 
                 −3413 
                 −2021 
                 546 
                 −2449 
                 −2273 
                 259 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 254(K) 
                 −1570 
                 −2144 
                 −1887 
                 −1191 
                 −2098 
                 −2363 
                 −750 
                 −1603 
                 3034 
                 938 
                 −1112 
                 −1231 
                 −2436 
                 −408 
                 215 
                 −1616 
                 −1443 
                 −1580 
                 −2166 
                 −1804 
                 260 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 255(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 261 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 256(R) 
                 −928 
                 −1705 
                 −1507 
                 −1055 
                 −2761 
                 −1730 
                 −896 
                 −2490 
                 −44 
                 −2489 
                 −1723 
                 −1042 
                 −2102 
                 −543 
                 2614 
                 2258 
                 −1053 
                 −1998 
                 −2546 
                 −2158 
                 262 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 257(D) 
                 −1280 
                 −2865 
                 3154 
                 175 
                 −3194 
                 −1547 
                 −743 
                 −3034 
                 −728 
                 −2971 
                 −2194 
                 −190 
                 −1979 
                 1342 
                 −1391 
                 553 
                 −1316 
                 −2552 
                 −3161 
                 −2317 
                 263 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 258(I) 
                 −1997 
                 −1562 
                 −4355 
                 −3927 
                 −1042 
                 −4066 
                 −3261 
                 3343 
                 −3654 
                 937 
                 97 
                 −3718 
                 −3783 
                 −3239 
                 −3555 
                 −3364 
                 −1959 
                 702 
                 −2549 
                 −2295 
                 264 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 259(M) 
                 −2252 
                 −1821 
                 −4572 
                 −3991 
                 −530 
                 −4164 
                 −2990 
                 2068 
                 −3709 
                 1993 
                 3197 
                 −3808 
                 −3685 
                 −2916 
                 −3406 
                 −3378 
                 −2149 
                 −172 
                 −2084 
                 −2091 
                 265 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 260(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 266 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 261(R) 
                 −2131 
                 −2786 
                 −2704 
                 −1460 
                 −3618 
                 −2638 
                 −587 
                 −2976 
                 1735 
                 −2645 
                 −1985 
                 −1353 
                 −2603 
                 −173 
                 3492 
                 −2020 
                 −1828 
                 −2748 
                 −2484 
                 −2384 
                 267 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 262(K) 
                 −1349 
                 −2635 
                 −381 
                 2083 
                 −3083 
                 −1857 
                 −565 
                 −2750 
                 2690 
                 −2612 
                 −1837 
                 −514 
                 −2090 
                 −161 
                 −61 
                 −1178 
                 −1271 
                 −2369 
                 −2655 
                 −2138 
                 268 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 263(A) 
                 2821 
                 −932 
                 −2451 
                 −2472 
                 −3065 
                 −1198 
                 −2233 
                 −2763 
                 −2434 
                 −3056 
                 −2201 
                 −1633 
                 −1940 
                 −2147 
                 −2468 
                 1831 
                 −730 
                 −1840 
                 −3305 
                 −3055 
                 269 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 264(F) 
                 −2063 
                 −1686 
                 −4037 
                 −3677 
                 3437 
                 −3644 
                 −1706 
                 2063 
                 −3359 
                 135 
                 67 
                 −3095 
                 −3486 
                 −2739 
                 −3127 
                 −2876 
                 −2012 
                 −83 
                 −1038 
                 −158 
                 270 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 265(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 271 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 266(N) 
                 −1662 
                 −3306 
                 2055 
                 78 
                 −3621 
                 −1643 
                 −1040 
                 −3622 
                 −1272 
                 −3531 
                 −2870 
                 3477 
                 −2182 
                 −724 
                 −2071 
                 −1371 
                 −1757 
                 −3092 
                 −3633 
                 −2700 
                 272 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 267(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 273 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 268(I) 
                 −1760 
                 −1307 
                 −4325 
                 −3962 
                 −1735 
                 −4042 
                 −3726 
                 3135 
                 −3828 
                 −579 
                 −515 
                 −3722 
                 −3869 
                 −3673 
                 −3896 
                 −3359 
                 −1752 
                 2276 
                 −3240 
                 −2806 
                 274 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 269(T) 
                 1428 
                 −904 
                 −2334 
                 −2158 
                 −2747 
                 −1206 
                 −1940 
                 −2392 
                 −2037 
                 −2678 
                 −1846 
                 −1504 
                 −1896 
                 −1809 
                 −2163 
                 902 
                 3001 
                 −1635 
                 −2999 
                 −2705 
                 275 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 270(V) 
                 −1745 
                 −1300 
                 −4286 
                 −3858 
                 −1446 
                 −3967 
                 −3370 
                 2358 
                 −3688 
                 852 
                 −261 
                 −3606 
                 −3749 
                 −3403 
                 −3673 
                 −3232 
                 −1717 
                 2643 
                 −2856 
                 −2524 
                 276 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 271(V) 
                 −1404 
                 −1072 
                 −3766 
                 −3305 
                 −1464 
                 −3356 
                 −2696 
                 2276 
                 −3080 
                 −616 
                 −379 
                 −3001 
                 −3325 
                 −2870 
                 −3091 
                 −2563 
                 1344 
                 2521 
                 −2516 
                 −2113 
                 277 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 272(M) 
                 866 
                 −1113 
                 −2656 
                 −2412 
                 −1322 
                 −1920 
                 −1883 
                 −487 
                 −2061 
                 −587 
                 4451 
                 −1950 
                 −2387 
                 −1928 
                 −2078 
                 −1220 
                 −1053 
                 −498 
                 −2134 
                 −1803 
                 278 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 273(A) 
                 2601 
                 −957 
                 −2898 
                 −2711 
                 −1943 
                 −1740 
                 −2211 
                 −165 
                 −2487 
                 −1406 
                 −1001 
                 −2008 
                 −2320 
                 −2260 
                 −2494 
                 −1053 
                 −929 
                 1990 
                 −2626 
                 −2279 
                 279 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 274(L) 
                 −1171 
                 −983 
                 −3266 
                 −2733 
                 −796 
                 −2795 
                 −1888 
                 590 
                 −2418 
                 2001 
                 198 
                 −2418 
                 −2816 
                 −2106 
                 −2362 
                 −1944 
                 965 
                 1777 
                 −1724 
                 −1426 
                 280 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 275(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 281 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 276(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 282 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 277(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 283 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 278(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 284 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 279(N) 
                 −2171 
                 −2655 
                 −1458 
                 −1748 
                 −3334 
                 −2364 
                 −2267 
                 −3943 
                 −2365 
                 −3936 
                 −3437 
                 4205 
                 −2932 
                 −2205 
                 −2608 
                 −2224 
                 −2439 
                 −3392 
                 −3253 
                 −2909 
                 285 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 280(A) 
                 3134 
                 −934 
                 −2491 
                 −2567 
                 −3083 
                 −1203 
                 −2300 
                 −2766 
                 −2540 
                 −3082 
                 −2237 
                 −1672 
                 −1954 
                 −2240 
                 −2537 
                 874 
                 −747 
                 −1844 
                 −3333 
                 −3093 
                 286 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 281(V) 
                 −984 
                 −1045 
                 −3169 
                 −2909 
                 −1709 
                 −2304 
                 −2404 
                 531 
                 −2643 
                 −988 
                 −697 
                 −2378 
                 −2722 
                 −2480 
                 −2661 
                 −1601 
                 1504 
                 3014 
                 −2588 
                 −2201 
                 287 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 282(L) 
                 −2631 
                 −2159 
                 −4786 
                 −4228 
                 −462 
                 −4506 
                 −3231 
                 96 
                 −3878 
                 2828 
                 2482 
                 −4157 
                 −3880 
                 −3016 
                 −3541 
                 −3793 
                 −2509 
                 −608 
                 −2134 
                 −2182 
                 288 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 283(H) 
                 −3205 
                 −3079 
                 −2723 
                 −2890 
                 −2110 
                 −3046 
                 5295 
                 −4135 
                 −2617 
                 −3813 
                 −3561 
                 −2886 
                 −3482 
                 −2833 
                 −2620 
                 −3291 
                 −3356 
                 −3895 
                 −2397 
                 −1681 
                 289 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 284(L) 
                 −1623 
                 −1338 
                 −3726 
                 −3164 
                 −251 
                 −3255 
                 −1820 
                 1373 
                 −2808 
                 2371 
                 514 
                 −2785 
                 −3086 
                 −2281 
                 −2613 
                 −2389 
                 −1543 
                 −161 
                 −1311 
                 1782 
                 290 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 285(L) 
                 −2333 
                 −1873 
                 −4640 
                 −4127 
                 −650 
                 −4326 
                 −3241 
                 2176 
                 −3843 
                 2519 
                 523 
                 −3982 
                 −3833 
                 −3105 
                 −3579 
                 −3604 
                 −2247 
                 56 
                 −2268 
                 −2230 
                 291 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 286(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 292 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 287(M) 
                 −1886 
                 −1507 
                 −4178 
                 −3693 
                 −877 
                 −3806 
                 −2901 
                 3008 
                 −3380 
                 335 
                 3109 
                 −3451 
                 −3570 
                 −2934 
                 −3251 
                 −3044 
                 −1840 
                 524 
                 −2288 
                 −2089 
                 293 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 288(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 294 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 289(H) 
                 −1490 
                 −2484 
                 −362 
                 −476 
                 −1816 
                 −1880 
                 4320 
                 −2854 
                 −684 
                 −2770 
                 −2133 
                 2185 
                 −2285 
                 −728 
                 −1000 
                 −1377 
                 −1550 
                 −2475 
                 −2146 
                 −1255 
                 295 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 290(A) 
                 2439 
                 −911 
                 −2326 
                 −2131 
                 −2811 
                 −1197 
                 −1934 
                 −2480 
                 −2011 
                 −2745 
                 −1898 
                 −1490 
                 −1888 
                 −1785 
                 −2153 
                 1898 
                 1073 
                 −1682 
                 −3044 
                 −2749 
                 296 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 291(I) 
                 2038 
                 −985 
                 −3388 
                 −2919 
                 −1320 
                 −2893 
                 −2277 
                 2155 
                 −2677 
                 −587 
                 −297 
                 −2593 
                 −2992 
                 −2450 
                 −2697 
                 −2087 
                 −1208 
                 1681 
                 −2229 
                 −1846 
                 297 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 292(G) 
                 −1243 
                 −2769 
                 311 
                 1902 
                 −3172 
                 1980 
                 −744 
                 −2992 
                 −697 
                 −2936 
                 −2152 
                 1923 
                 −1974 
                 −377 
                 −1331 
                 −1030 
                 −1284 
                 −2506 
                 −3125 
                 −2308 
                 298 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 293(V) 
                 −1738 
                 −1298 
                 −4281 
                 −3921 
                 −1737 
                 −3979 
                 −3665 
                 1917 
                 −3774 
                 −601 
                 −528 
                 −3671 
                 −3834 
                 −3628 
                 −3843 
                 −3293 
                 −1735 
                 3205 
                 −3215 
                 −2770 
                 299 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 294(E) 
                 −833 
                 −2344 
                 1092 
                 2412 
                 −2643 
                 −1464 
                 −386 
                 −2413 
                 −146 
                 −2369 
                 −1505 
                 −96 
                 562 
                 29 
                 −717 
                 −666 
                 862 
                 −1966 
                 −2562 
                 −1818 
                 300 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 295(W) 
                 −1380 
                 −1116 
                 −3614 
                 −3026 
                 1322 
                 −2981 
                 −1582 
                 1966 
                 −2661 
                 1775 
                 556 
                 −2562 
                 −2865 
                 −2117 
                 −2424 
                 −2098 
                 −1302 
                 −187 
                 2908 
                 −629 
                 301 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 296(T) 
                 −350 
                 −973 
                 −2204 
                 −2178 
                 −2893 
                 −1236 
                 −2035 
                 −2561 
                 −2117 
                 −2862 
                 −2043 
                 −1536 
                 −1946 
                 −1916 
                 −2214 
                 1618 
                 3198 
                 −1758 
                 −3137 
                 −2831 
                 302 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 297(L) 
                 −1443 
                 −1269 
                 −3144 
                 −2576 
                 −528 
                 −3014 
                 −1816 
                 1945 
                 −2155 
                 2102 
                 508 
                 −2422 
                 −2899 
                 1193 
                 −2133 
                 −2129 
                 −1369 
                 −50 
                 −1616 
                 −1384 
                 303 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 298(D) 
                 −1826 
                 −3682 
                 3559 
                 1199 
                 −3883 
                 −1662 
                 −1073 
                 −3846 
                 −1391 
                 −3720 
                 −3110 
                 −272 
                 −2222 
                 −760 
                 −2283 
                 −1471 
                 −1913 
                 −3321 
                 −3864 
                 −2864 
                 304 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 299(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 305 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 300(F) 
                 −3342 
                 −2776 
                 −4026 
                 −4232 
                 4354 
                 −3545 
                 −1431 
                 −2315 
                 −4038 
                 −1801 
                 −1900 
                 −3299 
                 −3780 
                 −3350 
                 −3645 
                 −3490 
                 −3420 
                 −2566 
                 −739 
                 349 
                 306 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 301(Q) 
                 −1048 
                 −2608 
                 205 
                 2170 
                 −2893 
                 −1535 
                 −505 
                 −2680 
                 −255 
                 −2604 
                 −1769 
                 1814 
                 −1849 
                 2272 
                 −789 
                 −848 
                 −1028 
                 −2228 
                 −2770 
                 −2013 
                 307 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 302(R) 
                 1083 
                 −1687 
                 691 
                 135 
                 −2058 
                 −1406 
                 −178 
                 −1755 
                 214 
                 −1793 
                 −924 
                 −145 
                 −1553 
                 247 
                 1670 
                 −383 
                 1217 
                 −1367 
                 −2031 
                 −1404 
                 308 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 303(I) 
                 −1915 
                 −1536 
                 −4077 
                 −3667 
                 2027 
                 −3678 
                 −2155 
                 3137 
                 −3381 
                 144 
                 94 
                 −3225 
                 −3506 
                 −2848 
                 −3202 
                 −2914 
                 −1871 
                 345 
                 −1522 
                 −791 
                 309 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 304(R) 
                 −689 
                 −2015 
                 −494 
                 24 
                 −2395 
                 −1582 
                 −184 
                 −2087 
                 444 
                 −2020 
                 −1151 
                 1161 
                 −1687 
                 1832 
                 2131 
                 626 
                 −614 
                 −1684 
                 −2156 
                 −1573 
                 310 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 305(D) 
                 387 
                 −1967 
                 1600 
                 1359 
                 −2275 
                 −1391 
                 1561 
                 −2025 
                 282 
                 −1976 
                 −1067 
                 −25 
                 −1525 
                 342 
                 1024 
                 −369 
                 −443 
                 −1584 
                 −2152 
                 −1462 
                 311 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 306(R) 
                 −1460 
                 −2315 
                 −1793 
                 −887 
                 −2832 
                 −2237 
                 −431 
                 −2288 
                 2193 
                 −2199 
                 −1473 
                 −946 
                 −2245 
                 −20 
                 2706 
                 −1394 
                 −1275 
                 591 
                 −2248 
                 −1961 
                 312 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 307(V) 
                 −941 
                 −1027 
                 −3099 
                 −2832 
                 −1692 
                 −2234 
                 −2324 
                 470 
                 −2565 
                 −1003 
                 −695 
                 −2305 
                 −2663 
                 −2399 
                 −2587 
                 −1527 
                 1858 
                 2876 
                 −2536 
                 −2152 
                 313 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 308(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 314 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 309(V) 
                 −1090 
                 −1215 
                 −2097 
                 −1824 
                 −819 
                 −2221 
                 2699 
                 −287 
                 −1392 
                 −1027 
                 −591 
                 −1674 
                 −2482 
                 −1446 
                 −1482 
                 −1482 
                 −1143 
                 2879 
                 −1420 
                 −707 
                 315 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 310(L) 
                 −2439 
                 −1972 
                 −4702 
                 −4181 
                 −588 
                 −4401 
                 −3258 
                 1582 
                 −3881 
                 2757 
                 587 
                 −4061 
                 −3862 
                 −3093 
                 −3590 
                 −3689 
                 −2344 
                 −130 
                 −2230 
                 −2217 
                 316 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 311(C) 
                 2157 
                 4166 
                 −3012 
                 −2973 
                 −2780 
                 1022 
                 −2337 
                 −2398 
                 −2724 
                 −2744 
                 −1930 
                 −1786 
                 −1943 
                 −2372 
                 −2623 
                 −540 
                 −692 
                 −1624 
                 −3091 
                 −2881 
                 317 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 312(D) 
                 −1732 
                 −3453 
                 3468 
                 99 
                 −3733 
                 −1645 
                 −1066 
                 −3747 
                 −1356 
                 −3641 
                 −3008 
                 1690 
                 −2201 
                 −755 
                 −2209 
                 −1416 
                 −1833 
                 −3208 
                 −3752 
                 −2776 
                 318 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 313(L) 
                 −2477 
                 −2023 
                 −4713 
                 −4122 
                 1592 
                 −4329 
                 −2920 
                 72 
                 −3835 
                 2593 
                 2472 
                 −3948 
                 −3754 
                 −2914 
                 −3466 
                 −3550 
                 −2350 
                 −634 
                 −1927 
                 −1830 
                 319 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 314(K) 
                 −2620 
                 −2961 
                 −2461 
                 −2046 
                 −3743 
                 −2791 
                 −1570 
                 −3603 
                 3784 
                 −3387 
                 −2839 
                 −2048 
                 −3039 
                 −1260 
                 −465 
                 −2604 
                 −2536 
                 −3331 
                 −3001 
                 −2988 
                 320 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 315(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 321 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 316(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 322 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 317(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 323 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 318(K) 
                 2 
                 −2257 
                 −1073 
                 −374 
                 −2740 
                 −1908 
                 −278 
                 −2339 
                 2328 
                 −2192 
                 −1373 
                 −562 
                 −1953 
                 2273 
                 1344 
                 −952 
                 −933 
                 −1980 
                 −2234 
                 −1799 
                 324 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 319(Y) 
                 −3482 
                 −2868 
                 −3701 
                 −3919 
                 238 
                 −3552 
                 −1112 
                 −3000 
                 −3638 
                 −2516 
                 −2526 
                 −3027 
                 −3772 
                 −3101 
                 −3341 
                 −3418 
                 −3527 
                 −3071 
                 −441 
                 4711 
                 325 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 320(M) 
                 −1559 
                 −1267 
                 −3829 
                 −3380 
                 −1103 
                 −3357 
                 −2655 
                 805 
                 −3067 
                 −64 
                 3046 
                 −3065 
                 −3326 
                 −2779 
                 −3011 
                 −2591 
                 −1556 
                 2855 
                 −2312 
                 −1998 
                 326 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 321(M) 
                 1225 
                 −469 
                 −2256 
                 −1679 
                 1656 
                 −1926 
                 −870 
                 90 
                 −1396 
                 −210 
                 2763 
                 −1424 
                 −2028 
                 −1129 
                 −1411 
                 −1008 
                 712 
                 154 
                 −951 
                 −586 
                 327 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 322(T) 
                 −738 
                 −2094 
                 −84 
                 1704 
                 −2416 
                 −1495 
                 −317 
                 −2135 
                 61 
                 −2127 
                 −1275 
                 −163 
                 −1704 
                 1857 
                 −405 
                 −613 
                 1930 
                 −1734 
                 −2331 
                 −1668 
                 328 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 323(D) 
                 −1746 
                 −3458 
                 3540 
                 90 
                 −3744 
                 −1650 
                 −1081 
                 −3767 
                 −1381 
                 −3662 
                 −3036 
                 1386 
                 −2211 
                 −772 
                 −2239 
                 −1429 
                 −1850 
                 −3226 
                 −3765 
                 −2789 
                 329 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 324(L) 
                 −2451 
                 −1983 
                 −4707 
                 −4186 
                 −582 
                 −4409 
                 −3259 
                 1510 
                 −3884 
                 2778 
                 592 
                 −4069 
                 −3865 
                 −3091 
                 −3590 
                 −3698 
                 −2355 
                 −150 
                 −2226 
                 −2214 
                 330 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 325(H) 
                 −2923 
                 −2573 
                 −2959 
                 −2926 
                 826 
                 −3449 
                 4553 
                 −2508 
                 −2463 
                 −2054 
                 −1948 
                 −2279 
                 −3499 
                 −2191 
                 −2397 
                 −2761 
                 −2855 
                 −2540 
                 123 
                 2920 
                 331 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 326(K) 
                 373 
                 −1957 
                 −342 
                 1025 
                 −2297 
                 −1472 
                 −98 
                 −2018 
                 2111 
                 −1954 
                 −1056 
                 906 
                 −1570 
                 352 
                 685 
                 −424 
                 −473 
                 −1592 
                 −2105 
                 −1469 
                 332 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 327(V) 
                 1739 
                 −1008 
                 −3509 
                 −3043 
                 −1376 
                 −3028 
                 −2406 
                 1765 
                 −2807 
                 −615 
                 −334 
                 −2718 
                 −3093 
                 −2585 
                 −2823 
                 −2226 
                 −1263 
                 2376 
                 −2322 
                 −1931 
                 333 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 328(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 334 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 329(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 335 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 330(I) 
                 −1758 
                 −1302 
                 −4331 
                 −3970 
                 −1756 
                 −4054 
                 −3748 
                 2976 
                 −3840 
                 −603 
                 −533 
                 −3731 
                 −3877 
                 −3693 
                 −3914 
                 −3372 
                 −1750 
                 2505 
                 −3265 
                 −2824 
                 336 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 331(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 337 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 332(Q) 
                 1795 
                 −1440 
                 −730 
                 −492 
                 −2453 
                 682 
                 −812 
                 −2151 
                 −508 
                 −2256 
                 −1426 
                 −624 
                 −1796 
                 2666 
                 −901 
                 −590 
                 −689 
                 −1636 
                 −2510 
                 −1971 
                 338 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 333(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 339 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 334(M) 
                 −2355 
                 −1988 
                 −4343 
                 −3834 
                 −504 
                 −4051 
                 −2868 
                 105 
                 −3385 
                 1451 
                 4460 
                 −3680 
                 −3671 
                 −2806 
                 −3171 
                 −3327 
                 −2274 
                 −474 
                 −2039 
                 −1925 
                 340 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 335(K) 
                 −2620 
                 −2961 
                 −2461 
                 −2046 
                 −3743 
                 −2791 
                 −1570 
                 −3603 
                 3784 
                 −3387 
                 −2839 
                 −2048 
                 −3039 
                 −1260 
                 −465 
                 −2604 
                 −2536 
                 −3331 
                 −3001 
                 −2988 
                 341 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 336(Y) 
                 −1187 
                 −974 
                 −3186 
                 −2638 
                 −117 
                 −2732 
                 −1255 
                 1905 
                 −2270 
                 73 
                 1977 
                 −2217 
                 −2699 
                 −1882 
                 −2144 
                 −1841 
                 −1124 
                 71 
                 −907 
                 3254 
                 342 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 337(L) 
                 −2871 
                 −2457 
                 −4231 
                 −4103 
                 −1033 
                 −3803 
                 −3165 
                 −541 
                 −3734 
                 3130 
                 −31 
                 −3935 
                 −3797 
                 −3286 
                 −3484 
                 −3713 
                 −2869 
                 −1136 
                 −2394 
                 −2220 
                 343 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 338(L) 
                 −2871 
                 −2457 
                 −4231 
                 −4103 
                 −1033 
                 −3803 
                 −3165 
                 −541 
                 −3734 
                 3130 
                 −31 
                 −3935 
                 −3797 
                 −3286 
                 −3484 
                 −3713 
                 −2869 
                 −1136 
                 −2394 
                 −2220 
                 344 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 339(K) 
                 −864 
                 −1785 
                 −860 
                 −366 
                 −2128 
                 −1763 
                 −407 
                 −1612 
                 2624 
                 −1800 
                 −1045 
                 629 
                 −1900 
                 −28 
                 62 
                 −851 
                 −805 
                 1127 
                 −2064 
                 −1581 
                 345 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 340(N) 
                 602 
                 −1686 
                 −275 
                 1008 
                 −1926 
                 −1415 
                 1528 
                 −1618 
                 244 
                 −1673 
                 −815 
                 1897 
                 −1530 
                 299 
                 −244 
                 −371 
                 −391 
                 322 
                 −1934 
                 −1306 
                 346 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 341(G) 
                 −1709 
                 −2639 
                 1362 
                 −690 
                 −3785 
                 3257 
                 −1671 
                 −3805 
                 −1946 
                 −3792 
                 −3137 
                 −980 
                 −2480 
                 −1424 
                 −2576 
                 −1630 
                 −1936 
                 −3150 
                 −3628 
                 −3155 
                 347 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 342(F) 
                 −942 
                 −799 
                 −2828 
                 −2226 
                 1797 
                 −2476 
                 −1269 
                 1109 
                 581 
                 1793 
                 516 
                 −1952 
                 −2453 
                 −1557 
                 −1815 
                 −1558 
                 −875 
                 52 
                 −1138 
                 −794 
                 348 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 343(L) 
                 −2451 
                 −1983 
                 −4707 
                 −4186 
                 −582 
                 −4409 
                 −3259 
                 1510 
                 −3884 
                 2778 
                 592 
                 −4069 
                 −3865 
                 −3091 
                 −3590 
                 −3698 
                 −2355 
                 −150 
                 −2226 
                 −2214 
                 349 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 344(H) 
                 −3205 
                 −3079 
                 −2723 
                 −2890 
                 −2110 
                 −3046 
                 5295 
                 −4135 
                 −2617 
                 −3813 
                 −3561 
                 −2886 
                 −3482 
                 −2833 
                 −2620 
                 −3291 
                 −3356 
                 −3895 
                 −2397 
                 −1681 
                 350 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 345(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 351 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 346(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 352 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 347(C) 
                 774 
                 4452 
                 −2162 
                 −1688 
                 −1962 
                 −1478 
                 −1302 
                 −1474 
                 −944 
                 −1796 
                 −1088 
                 −1351 
                 −1979 
                 −1147 
                 1684 
                 −732 
                 −719 
                 −1116 
                 −2225 
                 −1881 
                 353 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 348(L) 
                 −2387 
                 −1922 
                 −4674 
                 −4155 
                 −617 
                 −4366 
                 −3250 
                 1889 
                 −3865 
                 2650 
                 558 
                 −4023 
                 −3847 
                 −3098 
                 −3586 
                 −3647 
                 −2296 
                 −38 
                 −2247 
                 −2224 
                 354 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 349(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 355 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 350(C) 
                 −1489 
                 2972 
                 −4007 
                 −3563 
                 −1524 
                 −3541 
                 −2939 
                 2612 
                 −3350 
                 −617 
                 −413 
                 −3224 
                 −3470 
                 −3129 
                 −3335 
                 −2770 
                 −1475 
                 2269 
                 −2657 
                 −2248 
                 356 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 351(T) 
                 −364 
                 −979 
                 −2232 
                 −2250 
                 −2904 
                 −1245 
                 −2090 
                 −2559 
                 −2191 
                 −2881 
                 −2075 
                 −1571 
                 −1964 
                 −1991 
                 −2260 
                 905 
                 3428 
                 −1762 
                 −3159 
                 −2858 
                 357 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 352(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 358 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 353(K) 
                 −1716 
                 −2632 
                 −2004 
                 −1008 
                 −3336 
                 −2379 
                 −444 
                 −2764 
                 2775 
                 −2484 
                 −1756 
                 −1035 
                 −2357 
                 2151 
                 1811 
                 −1592 
                 −1477 
                 −2481 
                 −2391 
                 −2172 
                 359 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 354(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 360 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 355(V) 
                 −1771 
                 −1339 
                 −4275 
                 −3816 
                 −1235 
                 −3919 
                 −3194 
                 2139 
                 −3617 
                 1520 
                 −66 
                 −3558 
                 −3681 
                 −3244 
                 −3547 
                 −3164 
                 −1733 
                 2390 
                 −2634 
                 −2369 
                 361 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 356(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 362 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 357(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 363 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 358(N) 
                 −823 
                 −1917 
                 −96 
                 1188 
                 −2187 
                 −1547 
                 −506 
                 −1711 
                 −265 
                 −1955 
                 −1191 
                 2711 
                 −1815 
                 −144 
                 −747 
                 −757 
                 −815 
                 1140 
                 −2297 
                 −1666 
                 364 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 359(L) 
                 −2153 
                 −1779 
                 −4360 
                 −3884 
                 −675 
                 −3965 
                 −3012 
                 392 
                 −3561 
                 2726 
                 467 
                 −3673 
                 −3662 
                 −2955 
                 −3355 
                 −3239 
                 −2102 
                 1281 
                 −2207 
                 −2099 
                 365 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 360(E) 
                 1136 
                 −2084 
                 −175 
                 2027 
                 −2436 
                 −1510 
                 −274 
                 −2147 
                 1525 
                 −2118 
                 −1254 
                 −175 
                 −1692 
                 152 
                 −251 
                 −593 
                 −670 
                 −1736 
                 −2296 
                 −1650 
                 366 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 361(H) 
                 893 
                 −1761 
                 1357 
                 214 
                 −2092 
                 −1387 
                 1862 
                 −1810 
                 229 
                 −1825 
                 −942 
                 −83 
                 −1527 
                 293 
                 −273 
                 640 
                 793 
                 −1409 
                 −2050 
                 −1397 
                 367 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 362(I) 
                 608 
                 −458 
                 −2776 
                 −2176 
                 1666 
                 −2202 
                 −1113 
                 1712 
                 −1836 
                 −222 
                 338 
                 −1782 
                 −2245 
                 −1512 
                 −1731 
                 −1292 
                 867 
                 1366 
                 −1036 
                 −684 
                 368 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 363(P) 
                 −922 
                 −1912 
                 1681 
                 −141 
                 −2123 
                 −1604 
                 −687 
                 −1787 
                 −550 
                 187 
                 −1245 
                 −427 
                 2677 
                 −363 
                 −1049 
                 −882 
                 −947 
                 −1524 
                 −2338 
                 −1711 
                 369 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 364(D) 
                 −1692 
                 −3605 
                 3364 
                 1256 
                 −3770 
                 −1599 
                 −957 
                 −3700 
                 −1216 
                 −3569 
                 −2909 
                 1025 
                 −2138 
                 −628 
                 −2083 
                 −1346 
                 −1761 
                 −3174 
                 −3765 
                 −2738 
                 370 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 365(Q) 
                 −877 
                 −1646 
                 −633 
                 499 
                 −1610 
                 −1781 
                 −505 
                 −1210 
                 −63 
                 1648 
                 −649 
                 −558 
                 −1931 
                 2241 
                 −360 
                 −907 
                 −814 
                 −1097 
                 −1882 
                 −1385 
                 371 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 366(P) 
                 −648 
                 −2019 
                 1139 
                 203 
                 −2354 
                 −1436 
                 −285 
                 −2089 
                 29 
                 −2086 
                 −1217 
                 −114 
                 1965 
                 1445 
                 −492 
                 −529 
                 1244 
                 −1672 
                 −2300 
                 −1616 
                 372 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −571 
                 −7108 
                 −1646 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 367(R) 
                 −422 
                 −1009 
                 −851 
                 −304 
                 1406 
                 −1496 
                 −183 
                 −740 
                 147 
                 −894 
                 −230 
                 −440 
                 775 
                 21 
                 2009 
                 −539 
                 −381 
                 −568 
                 −1136 
                 −521 
                 373 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −23 
                 −6560 
                 −7602 
                 −894 
                 −1115 
                 −341 
                 −2249 
                 * 
                 * 
               
               
                 368(D) 
                 1472 
                 −1668 
                 1835 
                 −70 
                 −2356 
                 −1385 
                 −511 
                 −2062 
                 −246 
                 −2128 
                 −1275 
                 −318 
                 1353 
                 −118 
                 −746 
                 −526 
                 425 
                 −1602 
                 −2380 
                 −1752 
                 374 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 394 
                 45 
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                 — 
                 −16 
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                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 369(G) 
                 −1044 
                 −2230 
                 2141 
                 −100 
                 −3222 
                 2291 
                 −982 
                 −3045 
                 −1033 
                 −3050 
                 −2258 
                 −395 
                 −1985 
                 −644 
                 −1669 
                 858 
                 −1207 
                 −2428 
                 −3250 
                 −2493 
                 375 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 370(Q) 
                 −2562 
                 −2904 
                 −1886 
                 −1971 
                 −3251 
                 −2661 
                 −2079 
                 −3690 
                 −1565 
                 −3469 
                 −3081 
                 −2107 
                 −3091 
                 4371 
                 −1665 
                 −2585 
                 −2674 
                 −3411 
                 −3077 
                 −2821 
                 376 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
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                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 371(D) 
                 −1275 
                 −2955 
                 2862 
                 1330 
                 −3205 
                 −1556 
                 −670 
                 −3029 
                 1509 
                 −2936 
                 −2141 
                 −158 
                 −1955 
                 −290 
                 −1213 
                 −1025 
                 −1281 
                 −2554 
                 −3111 
                 −2272 
                 377 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 372(V) 
                 −1738 
                 −1298 
                 −4281 
                 −3921 
                 −1737 
                 −3979 
                 −3665 
                 1917 
                 −3774 
                 −601 
                 −528 
                 −3671 
                 −3834 
                 −3628 
                 −3843 
                 −3293 
                 −1735 
                 3205 
                 −3215 
                 −2770 
                 378 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
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                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 373(I) 
                 −2091 
                 −1746 
                 −3971 
                 −3840 
                 −1676 
                 −3532 
                 −3289 
                 3684 
                 −3581 
                 −659 
                 −693 
                 −3562 
                 −3674 
                 −3445 
                 −3521 
                 −3194 
                 −2146 
                 449 
                 −2877 
                 −2493 
                 379 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 374(M) 
                 −584 
                 −1354 
                 −847 
                 −246 
                 −1467 
                 −1659 
                 2505 
                 −1087 
                 212 
                 −374 
                 2571 
                 −449 
                 −1729 
                 1171 
                 1074 
                 −634 
                 −507 
                 −876 
                 −1617 
                 −1128 
                 380 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 375(P) 
                 −910 
                 −2031 
                 −73 
                 1195 
                 −2792 
                 −1488 
                 −794 
                 −2539 
                 −629 
                 −2588 
                 −1788 
                 −401 
                 3005 
                 −439 
                 −1131 
                 612 
                 −1014 
                 −2050 
                 −2815 
                 −2151 
                 381 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 376(W) 
                 −1588 
                 −1300 
                 −3783 
                 −3197 
                 −329 
                 −3245 
                 −1926 
                 2071 
                 −2827 
                 1901 
                 558 
                 −2822 
                 −3072 
                 −2297 
                 −2616 
                 −2381 
                 −1508 
                 −111 
                 3483 
                 −1042 
                 382 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 377(E) 
                 −1024 
                 −2640 
                 1844 
                 2310 
                 −2908 
                 −1498 
                 −505 
                 −2711 
                 −344 
                 −2636 
                 −1791 
                 −107 
                 −1824 
                 1521 
                 −957 
                 207 
                 −1011 
                 −2243 
                 −2817 
                 −2021 
                 383 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 378(N) 
                 −826 
                 −2349 
                 1089 
                 227 
                 −2651 
                 −1487 
                 −341 
                 −2416 
                 1494 
                 −2346 
                 −1475 
                 2601 
                 −1724 
                 1005 
                 −522 
                 −657 
                 −787 
                 −1968 
                 −2511 
                 −1791 
                 384 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 379(P) 
                 1932 
                 −1116 
                 −2232 
                 −2301 
                 −3058 
                 −1358 
                 −2206 
                 −2706 
                 −2336 
                 −3009 
                 −2238 
                 −1674 
                 3274 
                 −2114 
                 −2406 
                 −739 
                 −914 
                 −1913 
                 −3260 
                 −3019 
                 385 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 380(V) 
                 −914 
                 −773 
                 −2713 
                 −2129 
                 −712 
                 −2505 
                 −1388 
                 1452 
                 1084 
                 1324 
                 204 
                 −1926 
                 −2507 
                 −1580 
                 −1808 
                 −1591 
                 −859 
                 1713 
                 −1424 
                 −1081 
                 386 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 381(Y) 
                 −1484 
                 −2331 
                 −1762 
                 −887 
                 −2436 
                 −2254 
                 −420 
                 −2325 
                 2137 
                 −2195 
                 −1475 
                 −949 
                 −2258 
                 −39 
                 1983 
                 −1411 
                 −1295 
                 −2075 
                 −2087 
                 2868 
                 387 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 382(E) 
                 1256 
                 −1890 
                 −206 
                 1353 
                 −2196 
                 −1401 
                 −89 
                 −1930 
                 812 
                 −1898 
                 −996 
                 −45 
                 547 
                 1252 
                 −162 
                 −356 
                 −414 
                 −1507 
                 −2083 
                 −1416 
                 388 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 383(Q) 
                 −752 
                 −2272 
                 1586 
                 1407 
                 −2561 
                 −1448 
                 −308 
                 −2329 
                 −23 
                 −2276 
                 −1396 
                 −71 
                 −1677 
                 1749 
                 −577 
                 −590 
                 1569 
                 −1881 
                 −2459 
                 −1727 
                 389 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 384(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 390 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 385(H) 
                 −964 
                 −2089 
                 −200 
                 −136 
                 −2264 
                 −1600 
                 3833 
                 −2320 
                 −296 
                 −2338 
                 −1558 
                 1362 
                 1479 
                 −276 
                 −699 
                 −881 
                 −992 
                 −1924 
                 −2364 
                 −1652 
                 391 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 386(L) 
                 −2451 
                 −1983 
                 −4707 
                 −4186 
                 −582 
                 −4409 
                 −3259 
                 1510 
                 −3884 
                 2778 
                 592 
                 −4069 
                 −3865 
                 −3091 
                 −3590 
                 −3698 
                 −2355 
                 −150 
                 −2226 
                 −2214 
                 392 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 387(Q) 
                 1643 
                 −1017 
                 −1196 
                 −721 
                 −1189 
                 −1714 
                 −668 
                 1336 
                 −497 
                 −907 
                 −297 
                 −823 
                 −1893 
                 2044 
                 −794 
                 −784 
                 −569 
                 −339 
                 −1579 
                 −1135 
                 393 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 388(I) 
                 −1760 
                 −1308 
                 −4323 
                 −3961 
                 −1730 
                 −4039 
                 −3721 
                 3156 
                 −3825 
                 −575 
                 −512 
                 −3720 
                 −3867 
                 −3669 
                 −3893 
                 −3356 
                 −1753 
                 2241 
                 −3236 
                 −2802 
                 394 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 389(L) 
                 −2871 
                 −2457 
                 −4231 
                 −4103 
                 −1033 
                 −3803 
                 −3165 
                 −541 
                 −3734 
                 3130 
                 −31 
                 −3935 
                 −3797 
                 −3286 
                 −3484 
                 −3713 
                 −2869 
                 −1136 
                 −2394 
                 −2220 
                 395 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 390(K) 
                 −1259 
                 −2115 
                 −1267 
                 −676 
                 −970 
                 −2105 
                 1794 
                 −2040 
                 2549 
                 −1955 
                 −1282 
                 −808 
                 −2165 
                 −167 
                 114 
                 −1192 
                 −1140 
                 −1801 
                 −1301 
                 2517 
                 396 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 391(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 397 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 392(N) 
                 −2171 
                 −2655 
                 −1458 
                 −1748 
                 −3334 
                 −2364 
                 −2267 
                 −3943 
                 −2365 
                 −3936 
                 −3437 
                 4205 
                 −2932 
                 −2205 
                 −2608 
                 −2224 
                 −2439 
                 −3392 
                 −3253 
                 −2909 
                 398 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 393(L) 
                 −2871 
                 −2457 
                 −4231 
                 −4103 
                 −1033 
                 −3803 
                 −3165 
                 −541 
                 −3734 
                 3130 
                 −31 
                 −3935 
                 −3797 
                 −3286 
                 −3484 
                 −3713 
                 −2869 
                 −1136 
                 −2394 
                 −2220 
                 399 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 394(A) 
                 3121 
                 −934 
                 −2489 
                 −2561 
                 −3081 
                 −1203 
                 −2295 
                 −2766 
                 −2533 
                 −3080 
                 −2234 
                 −1669 
                 −1953 
                 −2234 
                 −2533 
                 936 
                 −746 
                 −1844 
                 −3331 
                 −3090 
                 400 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 395(E) 
                 −522 
                 −1773 
                 −240 
                 1676 
                 −2248 
                 −1396 
                 −289 
                 −1968 
                 50 
                 −1989 
                 −1115 
                 −174 
                 1198 
                 131 
                 −448 
                 1226 
                 677 
                 −1538 
                 −2214 
                 −1565 
                 401 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 396(E) 
                 −1481 
                 −3230 
                 1425 
                 2936 
                 −3481 
                 751 
                 −843 
                 −3354 
                 −954 
                 −3256 
                 −2520 
                 −187 
                 −2057 
                 −492 
                 −1711 
                 −1193 
                 −1527 
                 −2852 
                 −3445 
                 −2523 
                 402 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 397(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 403 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 398(A) 
                 2847 
                 −932 
                 −2454 
                 −2477 
                 −3066 
                 −1198 
                 −2236 
                 −2763 
                 −2439 
                 −3057 
                 −2202 
                 −1635 
                 −1940 
                 −2152 
                 −2471 
                 1777 
                 −731 
                 −1840 
                 −3306 
                 −3056 
                 404 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 399(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 405 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 400(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 406 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 401(K) 
                 −2620 
                 −2961 
                 −2461 
                 −2046 
                 −3743 
                 −2791 
                 −1570 
                 −3603 
                 3784 
                 −3387 
                 −2839 
                 −2048 
                 −3039 
                 −1260 
                 −465 
                 −2604 
                 −2536 
                 −3331 
                 −3001 
                 −2988 
                 407 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 402(I) 
                 −1761 
                 −1312 
                 −4317 
                 −3954 
                 −1713 
                 −4027 
                 −3703 
                 3225 
                 −3814 
                 −556 
                 −498 
                 −3712 
                 −3859 
                 −3653 
                 −3877 
                 −3344 
                 −1754 
                 2110 
                 −3216 
                 −2787 
                 408 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 403(S) 
                 −348 
                 −981 
                 −2200 
                 −2194 
                 −2989 
                 −1227 
                 −2073 
                 −2686 
                 −2157 
                 −2970 
                 −2136 
                 −1541 
                 −1946 
                 −1946 
                 −2253 
                 3060 
                 1398 
                 −1824 
                 −3217 
                 −2916 
                 409 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 404(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 410 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 405(V) 
                 −917 
                 −809 
                 −2556 
                 −1976 
                 −827 
                 −2491 
                 −1367 
                 1339 
                 1455 
                 721 
                 94 
                 −1841 
                 −2501 
                 −1487 
                 −1710 
                 −1570 
                 −863 
                 2038 
                 −1514 
                 −1151 
                 411 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 406(K) 
                 −1386 
                 −2643 
                 −447 
                 1824 
                 −3108 
                 −1893 
                 −570 
                 −2762 
                 2860 
                 −2616 
                 −1848 
                 −552 
                 −2117 
                 −166 
                 −3 
                 −1217 
                 −1300 
                 −2388 
                 −2647 
                 −2154 
                 412 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 407(N) 
                 −537 
                 −1563 
                 −449 
                 −36 
                 −1889 
                 1143 
                 −307 
                 −1529 
                 932 
                 −1655 
                 −844 
                 1794 
                 −1658 
                 73 
                 −356 
                 −518 
                 −516 
                 924 
                 −1962 
                 −1392 
                 413 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 408(P) 
                 −894 
                 −2181 
                 −369 
                 1705 
                 −2576 
                 −1650 
                 −357 
                 −2268 
                 243 
                 −2210 
                 −1375 
                 −330 
                 2093 
                 63 
                 1619 
                 −774 
                 −835 
                 −1876 
                 −2347 
                 −1769 
                 414 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 409(V) 
                 −419 
                 −634 
                 −1376 
                 −807 
                 1053 
                 −1737 
                 −499 
                 −198 
                 −623 
                 −505 
                 178 
                 600 
                 −1807 
                 −475 
                 475 
                 313 
                 −360 
                 1389 
                 −1016 
                 1303 
                 415 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 410(I) 
                 −1282 
                 −1082 
                 −3022 
                 −2555 
                 2426 
                 −2683 
                 1767 
                 2555 
                 −2191 
                 −443 
                 −88 
                 −2038 
                 −2692 
                 −1794 
                 −2075 
                 −1793 
                 −1220 
                 −317 
                 −361 
                 552 
                 416 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 411(T) 
                 −499 
                 −1595 
                 −431 
                 966 
                 −1830 
                 −1487 
                 −185 
                 −1449 
                 1092 
                 −1574 
                 −754 
                 −207 
                 −1601 
                 213 
                 −206 
                 −458 
                 2067 
                 159 
                 −1877 
                 −1296 
                 417 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 412(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 418 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 413(P) 
                 −632 
                 −1230 
                 −2074 
                 −2144 
                 −2996 
                 −1453 
                 −2116 
                 −2631 
                 −2128 
                 −2928 
                 −2213 
                 −1658 
                 3610 
                 −2006 
                 −2221 
                 −852 
                 1302 
                 −1931 
                 −3185 
                 −2917 
                 419 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 414(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 420 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 415(R) 
                 −1454 
                 −2316 
                 −1780 
                 −878 
                 −2834 
                 −2232 
                 −428 
                 −2292 
                 2281 
                 −2200 
                 −1473 
                 −940 
                 −2240 
                 −17 
                 2627 
                 −1386 
                 −1270 
                 588 
                 −2249 
                 −1960 
                 421 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 416(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 422 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 417(F) 
                 −3342 
                 −2776 
                 −4026 
                 −4232 
                 4354 
                 −3545 
                 −1431 
                 −2315 
                 −4038 
                 −1801 
                 −1900 
                 −3299 
                 −3780 
                 −3350 
                 −3645 
                 −3490 
                 −3420 
                 −2566 
                 −739 
                 349 
                 423 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 418(D) 
                 −1572 
                 −3426 
                 2573 
                 2447 
                 −3613 
                 −1583 
                 −879 
                 −3513 
                 −1050 
                 −3393 
                 −2684 
                 1292 
                 −2085 
                 −535 
                 −1855 
                 −1253 
                 −1623 
                 −3000 
                 −3585 
                 −2609 
                 424 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 419(S) 
                 −879 
                 −1989 
                 1498 
                 −177 
                 −3045 
                 1600 
                 −939 
                 −2843 
                 −904 
                 −2867 
                 −2046 
                 −438 
                 −1922 
                 −591 
                 −1483 
                 2171 
                 −1044 
                 −2226 
                 −3072 
                 −2372 
                 425 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 420(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 426 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 421(Q) 
                 −705 
                 −1925 
                 −199 
                 2112 
                 917 
                 −1534 
                 −288 
                 −1824 
                 42 
                 −1842 
                 −1054 
                 −210 
                 −1709 
                 2163 
                 −420 
                 −611 
                 −656 
                 −1502 
                 −1997 
                 −1291 
                 427 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 422(H) 
                 −569 
                 −2048 
                 1450 
                 1526 
                 −2349 
                 −1405 
                 1830 
                 −2103 
                 181 
                 −2058 
                 −1157 
                 −37 
                 −1569 
                 272 
                 −349 
                 713 
                 620 
                 −1662 
                 −2240 
                 −1537 
                 428 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 423(C) 
                 1626 
                 2878 
                 −2671 
                 −2107 
                 1264 
                 −1968 
                 −1091 
                 233 
                 −1777 
                 −334 
                 250 
                 −1672 
                 −2128 
                 −1459 
                 −1691 
                 −1096 
                 −529 
                 1209 
                 −1066 
                 −704 
                 429 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 424(M) 
                 −2042 
                 −1634 
                 −4379 
                 −3826 
                 −659 
                 −3976 
                 −2899 
                 2765 
                 −3546 
                 1204 
                 3085 
                 −3605 
                 −3604 
                 −2896 
                 −3318 
                 −3183 
                 −1961 
                 195 
                 −2135 
                 −2058 
                 430 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 425(E) 
                 412 
                 −2447 
                 1356 
                 2379 
                 −2747 
                 −1477 
                 −445 
                 −2527 
                 −243 
                 −2477 
                 −1622 
                 −107 
                 855 
                 −36 
                 −831 
                 −730 
                 −894 
                 −2073 
                 −2668 
                 −1906 
                 431 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 426(A) 
                 2822 
                 −1031 
                 −2418 
                 −2539 
                 −3226 
                 1898 
                 −2364 
                 −2941 
                 −2626 
                 −3229 
                 −2379 
                 −1722 
                 −2026 
                 −2302 
                 −2634 
                 −654 
                 −848 
                 −1983 
                 −3415 
                 −3226 
                 432 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 427(I) 
                 −1772 
                 −1325 
                 −4307 
                 −3877 
                 −1405 
                 −3993 
                 −3383 
                 2935 
                 −3705 
                 820 
                 −217 
                 −3632 
                 −3761 
                 −3400 
                 −3682 
                 −3260 
                 −1742 
                 2033 
                 −2838 
                 −2525 
                 433 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 428(L) 
                 −875 
                 −1634 
                 −575 
                 959 
                 −1581 
                 −1769 
                 −525 
                 −1179 
                 −135 
                 1884 
                 −625 
                 −547 
                 −1931 
                 1405 
                 −450 
                 −909 
                 −816 
                 −1074 
                 −1883 
                 −1383 
                 434 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 429(A) 
                 1705 
                 −1826 
                 −180 
                 949 
                 −2318 
                 −1410 
                 −359 
                 −2041 
                 −53 
                 −2067 
                 −1204 
                 1001 
                 −1652 
                 52 
                 −561 
                 1232 
                 −595 
                 −1609 
                 −2298 
                 −1643 
                 435 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 430(D) 
                 −1074 
                 −2458 
                 2381 
                 60 
                 −2921 
                 1927 
                 −658 
                 −2710 
                 −463 
                 −2675 
                 −1860 
                 −271 
                 −1918 
                 −276 
                 866 
                 −915 
                 −1100 
                 −2245 
                 −2845 
                 −2124 
                 436 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 431(K) 
                 −688 
                 −2117 
                 785 
                 888 
                 −2469 
                 −1529 
                 −187 
                 −2189 
                 2380 
                 −2106 
                 −1221 
                 −162 
                 −1661 
                 256 
                 1134 
                 −553 
                 −619 
                 −1760 
                 −2240 
                 −1607 
                 437 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 432(I) 
                 −2019 
                 −1582 
                 −4380 
                 −3941 
                 −1000 
                 −4086 
                 −3253 
                 3295 
                 −3671 
                 1100 
                 145 
                 −3736 
                 −3783 
                 −3222 
                 −3556 
                 −3378 
                 −1976 
                 657 
                 −2517 
                 −2289 
                 438 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 433(Q) 
                 −490 
                 −1797 
                 −369 
                 171 
                 −2078 
                 −1457 
                 1762 
                 −1779 
                 1157 
                 −1780 
                 −905 
                 1165 
                 −1550 
                 1798 
                 −48 
                 −396 
                 −422 
                 725 
                 −1986 
                 −1366 
                 439 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 434(A) 
                 1954 
                 −1836 
                 1733 
                 −180 
                 −2714 
                 −1429 
                 −806 
                 −2438 
                 −679 
                 −2518 
                 −1698 
                 −430 
                 1775 
                 −448 
                 −1211 
                 −736 
                 −894 
                 −1923 
                 −2765 
                 −2117 
                 440 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 435(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 441 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 436(D) 
                 −1736 
                 −3455 
                 3490 
                 97 
                 −3737 
                 −1646 
                 −1070 
                 −3753 
                 −1363 
                 −3647 
                 −3016 
                 1602 
                 −2204 
                 −760 
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                 −1838 
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                 −3756 
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                 442 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 −466 
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                 275 
                 394 
                 45 
                 96 
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                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 437(V) 
                 −1721 
                 −1302 
                 −4229 
                 −3874 
                 −1705 
                 −3894 
                 −3582 
                 1607 
                 −3706 
                 −582 
                 −513 
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                 −1725 
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                 443 
               
               
                 — 
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                 43 
                 −381 
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                 45 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 438(V) 
                 594 
                 −988 
                 −3391 
                 −2911 
                 −1164 
                 −2888 
                 −2187 
                 845 
                 −2637 
                 765 
                 −154 
                 −2576 
                 −2962 
                 −2387 
                 −2622 
                 −2074 
                 −1205 
                 2800 
                 −2084 
                 −1724 
                 444 
               
               
                 — 
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                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
                 394 
                 45 
                 96 
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                 117 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 439(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 445 
               
               
                 — 
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                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
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                 — 
                 −16 
                 −7108 
                 −8150 
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                 −1115 
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                 * 
                 * 
               
               
                 440(I) 
                 −1754 
                 −1308 
                 −4295 
                 −3867 
                 −1434 
                 −3978 
                 −3377 
                 2661 
                 −3697 
                 862 
                 −247 
                 −3617 
                 −3754 
                 −3406 
                 −3679 
                 −3243 
                 −1725 
                 2373 
                 −2852 
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                 446 
               
               
                 — 
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                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
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                 * 
                 * 
               
               
                 441(R) 
                 −2957 
                 −3022 
                 −3318 
                 −2735 
                 −3796 
                 −2998 
                 −1968 
                 −3912 
                 −846 
                 −3631 
                 −3157 
                 −2611 
                 −3280 
                 −1724 
                 4056 
                 −3026 
                 −2913 
                 −3650 
                 −3096 
                 −3185 
                 447 
               
               
                 — 
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                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 442(Y) 
                 −1321 
                 −1438 
                 −1994 
                 −1608 
                 2186 
                 527 
                 −450 
                 −1117 
                 −1481 
                 −1211 
                 −693 
                 1178 
                 −2522 
                 −1217 
                 −1665 
                 −1518 
                 −1275 
                 −1021 
                 −198 
                 3178 
                 448 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 443(C) 
                 −675 
                 2205 
                 −2544 
                 972 
                 −572 
                 −2236 
                 −1121 
                 1373 
                 −1671 
                 679 
                 261 
                 −1700 
                 −2270 
                 −1403 
                 −1668 
                 −1311 
                 −621 
                 1601 
                 −1150 
                 −790 
                 449 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
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                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 444(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
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                 450 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 445(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
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                 −3594 
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                 451 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 446(K) 
                 −1060 
                 −2058 
                 −1088 
                 −460 
                 −2432 
                 −1917 
                 −357 
                 −1970 
                 2801 
                 −1978 
                 −1220 
                 −632 
                 −1990 
                 1339 
                 367 
                 −999 
                 −946 
                 536 
                 −2145 
                 −1717 
                 452 
               
               
                 — 
                 −149 
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                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
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                 −701 
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                 * 
                 * 
               
               
                 447(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
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                 453 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
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                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 448(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
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                 −4004 
                 −3668 
                 −4222 
                 454 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 449(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 455 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
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                 — 
                 −16 
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                 −8150 
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                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 450(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 456 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 451(M) 
                 −2406 
                 −2296 
                 −3638 
                 −3594 
                 −1525 
                 −3105 
                 −2824 
                 −1047 
                 −3121 
                 −596 
                 5043 
                 −3293 
                 −3425 
                 −3046 
                 −2996 
                 −2911 
                 −2552 
                 −1398 
                 −2513 
                 −2207 
                 457 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 452(P) 
                 −1659 
                 −2241 
                 −2022 
                 −1646 
                 −3185 
                 −2242 
                 −1373 
                 −3000 
                 −450 
                 −2936 
                 −2274 
                 −1624 
                 3435 
                 −1065 
                 2095 
                 −1730 
                 −1750 
                 −2593 
                 −2816 
                 −2613 
                 458 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 453(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 459 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 454(M) 
                 −2406 
                 −2296 
                 −3638 
                 −3594 
                 −1525 
                 −3105 
                 −2824 
                 −1047 
                 −3121 
                 −596 
                 5043 
                 −3293 
                 −3425 
                 −3046 
                 −2996 
                 −2911 
                 −2552 
                 −1398 
                 −2513 
                 −2207 
                 460 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 455(L) 
                 −2871 
                 −2457 
                 −4231 
                 −4103 
                 −1033 
                 −3803 
                 −3165 
                 −541 
                 −3734 
                 3130 
                 −31 
                 −3935 
                 −3797 
                 −3286 
                 −3484 
                 −3713 
                 −2869 
                 −1136 
                 −2394 
                 −2220 
                 461 
               
               
                 — 
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                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 456(K) 
                 1368 
                 −1491 
                 −763 
                 −332 
                 −2319 
                 −1417 
                 −551 
                 −1998 
                 1786 
                 −2068 
                 −1221 
                 −500 
                 −1721 
                 −160 
                 −470 
                 1631 
                 −587 
                 −1532 
                 −2299 
                 −1754 
                 462 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 457(P) 
                 −1500 
                 −1738 
                 −2514 
                 −2380 
                 −1555 
                 −2358 
                 −2022 
                 −1126 
                 −2063 
                 1224 
                 −841 
                 −2189 
                 3436 
                 −2061 
                 −2129 
                 −1822 
                 −1674 
                 −1231 
                 −2290 
                 −1878 
                 463 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 458(T) 
                 −351 
                 −974 
                 −2208 
                 −2185 
                 −2894 
                 −1237 
                 −2041 
                 −2561 
                 −2125 
                 −2863 
                 −2046 
                 −1539 
                 −1948 
                 −1923 
                 −2218 
                 1543 
                 3230 
                 −1758 
                 −3139 
                 −2834 
                 464 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 459(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 465 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 460(M) 
                 2706 
                 −986 
                 −2433 
                 −2144 
                 −1502 
                 −1684 
                 −1706 
                 −700 
                 −1858 
                 −968 
                 2744 
                 −1705 
                 −2188 
                 −1713 
                 −1932 
                 −963 
                 −862 
                 −592 
                 −2145 
                 −1794 
                 466 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 461(I) 
                 −2103 
                 −1659 
                 −4461 
                 −3992 
                 −869 
                 −4152 
                 −3233 
                 3082 
                 −3723 
                 1619 
                 290 
                 −3801 
                 −3788 
                 −3171 
                 −3557 
                 −3432 
                 −2046 
                 487 
                 −2418 
                 −2265 
                 467 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 462(I) 
                 −1761 
                 −1312 
                 −4317 
                 −3954 
                 −1713 
                 −4027 
                 −3703 
                 3225 
                 −3814 
                 −556 
                 −498 
                 −3712 
                 −3859 
                 −3653 
                 −3877 
                 −3344 
                 −1754 
                 2110 
                 −3216 
                 −2787 
                 468 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 463(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 469 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 464(K) 
                 1641 
                 −2033 
                 −323 
                 914 
                 −2415 
                 −1565 
                 −296 
                 −2097 
                 2052 
                 −2080 
                 −1233 
                 −257 
                 −1736 
                 125 
                 −133 
                 −646 
                 −702 
                 −1707 
                 −2258 
                 −1657 
                 470 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 465(G) 
                 −2594 
                 −2690 
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                 — 
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                 43 
                 −381 
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                 275 
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                 45 
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                 −369 
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                 — 
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                 −7108 
                 −8150 
                 −894 
                 −1115 
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                 −1378 
                 * 
                 * 
               
               
                 466(L) 
                 −1699 
                 −1807 
                 −2268 
                 −1925 
                 −830 
                 −2795 
                 −1551 
                 −455 
                 −1225 
                 2510 
                 90 
                 −1958 
                 −2845 
                 1927 
                 −1308 
                 −2067 
                 −1651 
                 −846 
                 −1841 
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                 — 
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                 43 
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                 45 
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                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
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                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 467(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
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                 −3779 
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                 −2981 
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                 −3668 
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                 — 
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                 43 
                 −381 
                 399 
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                 −626 
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                 275 
                 394 
                 45 
                 96 
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                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 468(D) 
                 −853 
                 −2415 
                 2115 
                 1717 
                 −2702 
                 −1468 
                 −378 
                 −2484 
                 1085 
                 −2417 
                 −1546 
                 −84 
                 −1732 
                 41 
                 −699 
                 696 
                 −824 
                 −2025 
                 −2594 
                 −1839 
                 474 
               
               
                 — 
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                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
                 394 
                 45 
                 96 
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                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 469(S) 
                 −892 
                 −1780 
                 −931 
                 −688 
                 −2757 
                 −1643 
                 −830 
                 −2472 
                 1671 
                 −2492 
                 −1708 
                 −799 
                 −2018 
                 −468 
                 −365 
                 2676 
                 −1004 
                 −1981 
                 −2598 
                 −2130 
                 475 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 470(C) 
                 −1135 
                 3503 
                 −3700 
                 −3406 
                 −1670 
                 −2549 
                 −2675 
                 653 
                 −3101 
                 −916 
                 −667 
                 −2727 
                 −2925 
                 −2870 
                 −3030 
                 −1868 
                 −1288 
                 2927 
                 −2619 
                 −2222 
                 476 
               
               
                 — 
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                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 471(A) 
                 2590 
                 −1035 
                 −2404 
                 −2530 
                 −3236 
                 2290 
                 −2365 
                 −2954 
                 −2627 
                 −3240 
                 −2389 
                 −1719 
                 −2027 
                 −2302 
                 −2637 
                 −656 
                 −851 
                 −1991 
                 −3423 
                 −3234 
                 477 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 472(L) 
                 −2632 
                 −2152 
                 −4630 
                 −4185 
                 1767 
                 −4324 
                 −2442 
                 −61 
                 −3879 
                 2789 
                 563 
                 −3833 
                 −3823 
                 −2970 
                 −3513 
                 −3609 
                 −2518 
                 −738 
                 −1527 
                 −945 
                 478 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 473(I) 
                 −2073 
                 −1632 
                 −4434 
                 −3975 
                 −911 
                 −4130 
                 −3238 
                 3164 
                 −3706 
                 1451 
                 244 
                 −3779 
                 −3785 
                 −3187 
                 −3557 
                 −3413 
                 −2021 
                 546 
                 −2449 
                 −2273 
                 479 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 474(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 480 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 475(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 481 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 476(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 482 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 477(R) 
                 −2957 
                 −3022 
                 −3318 
                 −2735 
                 −3796 
                 −2998 
                 −1968 
                 −3912 
                 −846 
                 −3631 
                 −3157 
                 −2611 
                 −3280 
                 −1724 
                 4056 
                 −3026 
                 −2913 
                 −3650 
                 −3096 
                 −3185 
                 483 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 478(F) 
                 −3342 
                 −2776 
                 −4026 
                 −4232 
                 4354 
                 −3545 
                 −1431 
                 −2315 
                 −4038 
                 −1801 
                 −1900 
                 −3299 
                 −3780 
                 −3350 
                 −3645 
                 −3490 
                 −3420 
                 −2566 
                 −739 
                 349 
                 484 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 479(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 485 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 480(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 486 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 481(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 487 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 482(T) 
                 −359 
                 −976 
                 −2225 
                 −2229 
                 −2900 
                 −1242 
                 −2074 
                 −2560 
                 −2170 
                 −2875 
                 −2064 
                 −1561 
                 −1958 
                 −1969 
                 −2247 
                 1110 
                 3375 
                 −1760 
                 −3152 
                 −2850 
                 488 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 483(Y) 
                 −3402 
                 −2632 
                 −3941 
                 −4011 
                 1064 
                 −3924 
                 3388 
                 −2526 
                 −3541 
                 −1996 
                 −1973 
                 −2625 
                 −3821 
                 −2664 
                 −3170 
                 −3135 
                 −3280 
                 −2619 
                 3420 
                 3756 
                 489 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 484(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 490 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 485(M) 
                 −2322 
                 −1904 
                 −4536 
                 −3951 
                 2387 
                 −4112 
                 −2676 
                 67 
                 −3649 
                 2034 
                 3156 
                 −3710 
                 −3633 
                 −2803 
                 −3311 
                 −3309 
                 −2204 
                 −588 
                 −1794 
                 −1586 
                 491 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 486(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 492 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 487(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 493 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 488(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 494 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 489(H) 
                 −3205 
                 −3079 
                 −2723 
                 −2890 
                 −2110 
                 −3046 
                 5295 
                 −4135 
                 −2617 
                 −3813 
                 −3561 
                 −2886 
                 −3482 
                 −2833 
                 −2620 
                 −3291 
                 −3356 
                 −3895 
                 −2397 
                 −1681 
                 495 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 490(V) 
                 −1754 
                 −1297 
                 −4329 
                 −3968 
                 −1770 
                 −4053 
                 −3752 
                 2604 
                 −3840 
                 −621 
                 −545 
                 −3728 
                 −3878 
                 −3699 
                 −3917 
                 −3370 
                 −1746 
                 2859 
                 −3276 
                 −2829 
                 496 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 491(A) 
                 2587 
                 −828 
                 −2477 
                 −2155 
                 −1837 
                 −1468 
                 −1728 
                 −743 
                 −1941 
                 −1564 
                 −954 
                 −1607 
                 −2033 
                 −1725 
                 −2034 
                 −738 
                 1178 
                 1108 
                 −2310 
                 −1972 
                 497 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 492(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 498 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 493(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 499 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 494(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 500 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 495(Y) 
                 −866 
                 −976 
                 −1863 
                 −1331 
                 1353 
                 −2145 
                 1318 
                 −556 
                 −1116 
                 −777 
                 −173 
                 −1242 
                 −2197 
                 1714 
                 −1301 
                 −1173 
                 −802 
                 888 
                 −445 
                 2749 
                 501 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 496(D) 
                 417 
                 −1831 
                 1647 
                 1094 
                 −2065 
                 −1488 
                 −353 
                 −1618 
                 −107 
                 −1820 
                 −1019 
                 −189 
                 −1698 
                 30 
                 −623 
                 −603 
                 −643 
                 1629 
                 −2154 
                 −1520 
                 502 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 497(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 503 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 498(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 504 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 499(T) 
                 492 
                 −1190 
                 −706 
                 −181 
                 −1475 
                 311 
                 −333 
                 −1099 
                 −81 
                 71 
                 −509 
                 570 
                 1113 
                 −6 
                 −509 
                 −450 
                 1123 
                 −835 
                 −1680 
                 −1161 
                 505 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 500(I) 
                 −2091 
                 −1746 
                 −3971 
                 −3840 
                 −1676 
                 −3532 
                 −3289 
                 3684 
                 −3581 
                 −659 
                 −693 
                 −3562 
                 −3674 
                 −3445 
                 −3521 
                 −3194 
                 −2146 
                 449 
                 −2877 
                 −2493 
                 506 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 501(A) 
                 3103 
                 −1036 
                 −2445 
                 −2572 
                 −3222 
                 1051 
                 −2380 
                 −2930 
                 −2650 
                 −3226 
                 −2381 
                 −1739 
                 −2034 
                 −2327 
                 −2648 
                 −664 
                 −857 
                 −1981 
                 −3412 
                 −3228 
                 507 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 502(L) 
                 −2239 
                 −1892 
                 −3711 
                 −3400 
                 301 
                 −3520 
                 −1210 
                 −542 
                 −2948 
                 2564 
                 −35 
                 −2786 
                 −3395 
                 −2438 
                 −2750 
                 −2747 
                 −2165 
                 −945 
                 −573 
                 2562 
                 508 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 503(V) 
                 −1757 
                 −1387 
                 −4101 
                 −3681 
                 −1174 
                 −3714 
                 −3031 
                 880 
                 −3410 
                 1254 
                 −60 
                 −3407 
                 −3585 
                 −3094 
                 −3354 
                 −2984 
                 −1743 
                 3014 
                 −2536 
                 −2219 
                 509 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 504(Q) 
                 −982 
                 −2251 
                 −866 
                 971 
                 −2711 
                 −1822 
                 −252 
                 −2340 
                 1444 
                 −2194 
                 −1356 
                 −464 
                 −1885 
                 2646 
                 1632 
                 −858 
                 −863 
                 −1958 
                 −2245 
                 −1765 
                 510 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 505(E) 
                 −1162 
                 −2771 
                 2137 
                 2239 
                 −3046 
                 −1526 
                 −626 
                 −2849 
                 −546 
                 −2792 
                 −1983 
                 −145 
                 −1905 
                 −242 
                 −1192 
                 −940 
                 1396 
                 −2385 
                 −2990 
                 −2169 
                 511 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 506(G) 
                 −1707 
                 −2684 
                 1591 
                 −614 
                 −3783 
                 3190 
                 −1613 
                 −3795 
                 −1887 
                 −3775 
                 −3119 
                 −915 
                 −2456 
                 −1358 
                 −2539 
                 −1610 
                 −1924 
                 −3150 
                 −3636 
                 −3124 
                 512 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 507(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 513 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 508(M) 
                 −473 
                 −522 
                 −1819 
                 −1236 
                 −468 
                 −1879 
                 −687 
                 1519 
                 −996 
                 566 
                 1677 
                 −1154 
                 −1937 
                 836 
                 −1131 
                 1079 
                 −413 
                 102 
                 −957 
                 −585 
                 514 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 509(I) 
                 −1761 
                 −1312 
                 −4317 
                 −3954 
                 −1713 
                 −4027 
                 −3703 
                 3225 
                 −3814 
                 −556 
                 −498 
                 −3712 
                 −3859 
                 −3653 
                 −3877 
                 −3344 
                 −1754 
                 2110 
                 −3216 
                 −2787 
                 515 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 510(T) 
                 782 
                 −1467 
                 −550 
                 1029 
                 −2202 
                 −1425 
                 −709 
                 −1791 
                 −472 
                 −1993 
                 −1203 
                 −528 
                 −1787 
                 −368 
                 −902 
                 −617 
                 2685 
                 −1400 
                 −2333 
                 −1783 
                 516 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 511(I) 
                 −1766 
                 −1333 
                 −4283 
                 −3923 
                 −1635 
                 −3967 
                 −3619 
                 3388 
                 −3759 
                 −473 
                 −437 
                 −3672 
                 −3822 
                 −3576 
                 −3804 
                 −3285 
                 −1764 
                 1695 
                 −3126 
                 −2717 
                 517 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 512(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 518 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 513(A) 
                 2705 
                 −1451 
                 −1036 
                 −913 
                 −2506 
                 −1504 
                 −1143 
                 −2174 
                 −794 
                 −2337 
                 −1613 
                 −946 
                 −1993 
                 2040 
                 −1061 
                 −809 
                 −910 
                 −1703 
                 −2633 
                 −2156 
                 519 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 514(H) 
                 −615 
                 −1680 
                 1444 
                 66 
                 −1883 
                 168 
                 2650 
                 −1558 
                 −86 
                 −1691 
                 −891 
                 −223 
                 −1680 
                 31 
                 −577 
                 −571 
                 −585 
                 1267 
                 −2007 
                 −1397 
                 520 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 515(K) 
                 −654 
                 −2006 
                 −546 
                 42 
                 −2376 
                 −1581 
                 −133 
                 −2066 
                 1935 
                 −1987 
                 −1107 
                 1132 
                 −1658 
                 1043 
                 1058 
                 −540 
                 1180 
                 −1660 
                 −2113 
                 −1532 
                 521 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 516(N) 
                 −933 
                 −2085 
                 −946 
                 −284 
                 −2472 
                 −1822 
                 −253 
                 −2090 
                 1711 
                 76 
                 −1204 
                 1918 
                 −1876 
                 175 
                 1799 
                 −841 
                 −817 
                 −1755 
                 −2132 
                 −1663 
                 522 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 517(E) 
                 −416 
                 −987 
                 −843 
                 1107 
                 −1070 
                 −1583 
                 −338 
                 −623 
                 −183 
                 879 
                 −172 
                 −489 
                 −1679 
                 −94 
                 −565 
                 544 
                 813 
                 265 
                 −1379 
                 −905 
                 523 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 518(I) 
                 −2258 
                 −1804 
                 −4588 
                 −4084 
                 −706 
                 −4269 
                 −3231 
                 2527 
                 −3807 
                 2292 
                 465 
                 −3923 
                 −3814 
                 −3118 
                 −3570 
                 −3544 
                 −2181 
                 190 
                 −2303 
                 −2237 
                 524 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 519(Q) 
                 −477 
                 −1909 
                 958 
                 282 
                 −2211 
                 −1389 
                 1484 
                 −1953 
                 285 
                 −1921 
                 −1018 
                 −32 
                 −1517 
                 2318 
                 −225 
                 630 
                 559 
                 −1525 
                 −2110 
                 −1430 
                 525 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 520(L) 
                 −2127 
                 −1743 
                 −4402 
                 −3796 
                 1257 
                 −3918 
                 −2674 
                 149 
                 −3492 
                 2527 
                 2164 
                 −3553 
                 −3509 
                 −2714 
                 −3181 
                 −3095 
                 −2019 
                 570 
                 −1870 
                 −1818 
                 526 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 521(N) 
                 −723 
                 −2217 
                 958 
                 236 
                 −2518 
                 −1466 
                 1611 
                 −2279 
                 1719 
                 −2217 
                 −1334 
                 2285 
                 −1666 
                 166 
                 −401 
                 −570 
                 −677 
                 −1837 
                 −2382 
                 −1678 
                 527 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 522(V) 
                 −1754 
                 −1297 
                 −4330 
                 −3968 
                 −1770 
                 −4053 
                 −3752 
                 2623 
                 −3841 
                 −620 
                 −545 
                 −3729 
                 −3878 
                 −3699 
                 −3918 
                 −3371 
                 −1746 
                 2846 
                 −3277 
                 −2830 
                 528 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 523(S) 
                 1545 
                 −974 
                 −2003 
                 −1825 
                 −2867 
                 −1206 
                 −1790 
                 −2580 
                 −1788 
                 −2795 
                 −1932 
                 −1362 
                 1826 
                 −1586 
                 −1999 
                 2362 
                 −672 
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                 −3057 
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                 — 
                 −149 
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                 233 
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                 — 
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                 * 
                 * 
               
               
                 524(D) 
                 −1776 
                 −3649 
                 3326 
                 1869 
                 −3838 
                 −1642 
                 −1031 
                 −3788 
                 −1322 
                 −3660 
                 −3029 
                 −245 
                 −2192 
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                 −2201 
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                 −1855 
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                 — 
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                 45 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
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                 * 
                 * 
               
               
                 525(E) 
                 423 
                 −2950 
                 1944 
                 2696 
                 −3223 
                 −1545 
                 −718 
                 −3047 
                 −715 
                 −2979 
                 −2196 
                 −161 
                 −1968 
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                 −1403 
                 −1043 
                 −1314 
                 −2569 
                 −3177 
                 −2316 
                 531 
               
               
                 — 
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                 43 
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                 106 
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                 275 
                 394 
                 45 
                 96 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
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                 * 
                 * 
               
               
                 526(E) 
                 −2641 
                 −3308 
                 −896 
                 3732 
                 −3966 
                 −2458 
                 −2043 
                 −4105 
                 −2128 
                 −4016 
                 −3555 
                 −1531 
                 −2959 
                 −1842 
                 −2560 
                 −2479 
                 −2750 
                 −3722 
                 −3563 
                 −3385 
                 532 
               
               
                 — 
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                 43 
                 −381 
                 399 
                 106 
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                 275 
                 394 
                 45 
                 96 
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                 — 
                 −16 
                 −7108 
                 −8150 
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                 −1115 
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                 * 
                 * 
               
               
                 527(L) 
                 −2339 
                 −1899 
                 −4618 
                 −4042 
                 1570 
                 −4204 
                 −2849 
                 1440 
                 −3758 
                 2558 
                 676 
                 −3825 
                 −3700 
                 −2902 
                 −3418 
                 −3418 
                 −2226 
                 −382 
                 −1924 
                 −1778 
                 533 
               
               
                 — 
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                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 528(A) 
                 2338 
                 −1990 
                 −241 
                 938 
                 −2395 
                 −1557 
                 −423 
                 −2061 
                 954 
                 −2103 
                 −1286 
                 −301 
                 −1791 
                 −26 
                 −375 
                 −717 
                 −784 
                 −1691 
                 −2330 
                 −1728 
                 534 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 529(R) 
                 524 
                 −2098 
                 −789 
                 −146 
                 −2504 
                 −1729 
                 1632 
                 −2153 
                 1229 
                 −2054 
                 −1204 
                 −379 
                 −1789 
                 1328 
                 2313 
                 −719 
                 −724 
                 −1774 
                 −2150 
                 −1637 
                 535 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 530(R) 
                 −2957 
                 −3022 
                 −3318 
                 −2735 
                 −3796 
                 −2998 
                 −1968 
                 −3912 
                 −846 
                 −3631 
                 −3157 
                 −2611 
                 −3280 
                 −1724 
                 4056 
                 −3026 
                 −2913 
                 −3650 
                 −3096 
                 −3185 
                 536 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 531(R) 
                 −1895 
                 −2713 
                 −2327 
                 −1192 
                 −3484 
                 −2502 
                 −481 
                 −2856 
                 2144 
                 −2544 
                 −1842 
                 −1161 
                 −2458 
                 1393 
                 3023 
                 −1770 
                 −1619 
                 −2599 
                 −2421 
                 −2259 
                 537 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 532(A) 
                 2935 
                 −1714 
                 −553 
                 857 
                 −2769 
                 −1546 
                 −1218 
                 −2333 
                 −1106 
                 −2591 
                 −1873 
                 −809 
                 −2065 
                 −934 
                 −1502 
                 −954 
                 −1103 
                 −1872 
                 −2898 
                 −2374 
                 538 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 533(A) 
                 1291 
                 −1874 
                 −176 
                 1227 
                 −2177 
                 −1392 
                 −109 
                 −1909 
                 277 
                 −1891 
                 −995 
                 1134 
                 −1522 
                 1248 
                 −228 
                 −361 
                 562 
                 −1492 
                 −2090 
                 −1419 
                 539 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 534(W) 
                 −805 
                 −687 
                 −2581 
                 −2028 
                 138 
                 −2236 
                 −697 
                 897 
                 −1681 
                 −421 
                 141 
                 −1645 
                 −2282 
                 −1369 
                 −1627 
                 −1315 
                 636 
                 −90 
                 4479 
                 1809 
                 540 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 535(H) 
                 −408 
                 −1801 
                 −274 
                 1284 
                 −2096 
                 −1385 
                 1500 
                 −1822 
                 1168 
                 −1802 
                 −899 
                 −33 
                 −1479 
                 1381 
                 −102 
                 −303 
                 595 
                 221 
                 −1996 
                 −1339 
                 541 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 536(Q) 
                 −650 
                 −1737 
                 −627 
                 −72 
                 −1981 
                 −1615 
                 −209 
                 −1625 
                 1223 
                 392 
                 −866 
                 −318 
                 1222 
                 2120 
                 50 
                 −598 
                 −572 
                 −1326 
                 −1932 
                 −1394 
                 542 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 537(P) 
                 −2931 
                 −2878 
                 −3420 
                 −3706 
                 −4181 
                 −2925 
                 −3468 
                 −4621 
                 −3859 
                 −4490 
                 −4165 
                 −3491 
                 4225 
                 −3781 
                 −3695 
                 −3182 
                 −3279 
                 −4087 
                 −3594 
                 −4064 
                 543 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
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                 — 
                 −324 
                 −7108 
                 −2368 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 538(A) 
                 2195 
                 −924 
                 −968 
                 −546 
                 −1397 
                 −1356 
                 −583 
                 −812 
                 −365 
                 −1167 
                 −487 
                 −618 
                 −1660 
                 1324 
                 −684 
                 −483 
                 −404 
                 462 
                 −1703 
                 −1242 
                 544 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −19 
                 −6804 
                 −7846 
                 −894 
                 −1115 
                 −428 
                 −1961 
                 * 
                 * 
               
               
                 539(P) 
                 411 
                 −1017 
                 −1886 
                 −1616 
                 −1600 
                 −1588 
                 −1411 
                 −962 
                 −1408 
                 495 
                 −755 
                 −1384 
                 3156 
                 −1323 
                 −1577 
                 −847 
                 −785 
                 −783 
                 −2111 
                 −1716 
                 545 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 540(R) 
                 −1612 
                 −2397 
                 −2037 
                 −1033 
                 −2897 
                 −2352 
                 −458 
                 −2365 
                 2184 
                 665 
                 −1520 
                 −1051 
                 −2334 
                 −51 
                 2602 
                 −1545 
                 −1395 
                 −2143 
                 −2262 
                 −2014 
                 546 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 541(Y) 
                 712 
                 −796 
                 −2334 
                 −1883 
                 −370 
                 −2028 
                 −986 
                 −143 
                 −1607 
                 −663 
                 −131 
                 −1587 
                 −2243 
                 −1383 
                 −1656 
                 −1178 
                 −771 
                 1114 
                 −965 
                 3479 
                 547 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
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                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 542(T) 
                 −527 
                 −1669 
                 1091 
                 −27 
                 −2315 
                 −1379 
                 −443 
                 −2033 
                 −151 
                 −2081 
                 −1218 
                 −282 
                 557 
                 −41 
                 −650 
                 1128 
                 2077 
                 −1576 
                 −2321 
                 −1690 
                 548 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
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                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
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                 — 
                 −16 
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                 −8150 
                 −894 
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                 * 
                 * 
               
               
                 543(R) 
                 −2957 
                 −3022 
                 −3318 
                 −2735 
                 −3796 
                 −2998 
                 −1968 
                 −3912 
                 −846 
                 −3631 
                 −3157 
                 −2611 
                 −3280 
                 −1724 
                 4056 
                 −3026 
                 −2913 
                 −3650 
                 −3096 
                 −3185 
                 549 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
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                 — 
                 −16 
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                 −8150 
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                 * 
                 * 
               
               
                 544(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 550 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 545(V) 
                 −1747 
                 −1296 
                 −4310 
                 −3948 
                 −1758 
                 −4023 
                 −3716 
                 2215 
                 −3813 
                 −615 
                 −540 
                 −3705 
                 −3860 
                 −3670 
                 −3887 
                 −3339 
                 −1741 
                 3087 
                 −3252 
                 −2806 
                 551 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
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                 — 
                 −16 
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                 −8150 
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                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 546(L) 
                 −2871 
                 −2457 
                 −4231 
                 −4103 
                 −1033 
                 −3803 
                 −3165 
                 −541 
                 −3734 
                 3130 
                 −31 
                 −3935 
                 −3797 
                 −3286 
                 −3484 
                 −3713 
                 −2869 
                 −1136 
                 −2394 
                 −2220 
                 552 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
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                 — 
                 −16 
                 −7108 
                 −8150 
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                 −1115 
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                 * 
                 * 
               
               
                 547(A) 
                 2404 
                 −890 
                 −1926 
                 −1629 
                 −1803 
                 1275 
                 −1415 
                 −1282 
                 −1490 
                 392 
                 −963 
                 −1316 
                 −1930 
                 −1328 
                 −1674 
                 −654 
                 −644 
                 −952 
                 −2187 
                 −1810 
                 553 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 548(K) 
                 −2620 
                 −2961 
                 −2461 
                 −2046 
                 −3743 
                 −2791 
                 −1570 
                 −3603 
                 3784 
                 −3387 
                 −2839 
                 −2048 
                 −3039 
                 −1260 
                 −465 
                 −2604 
                 −2536 
                 −3331 
                 −3001 
                 −2988 
                 554 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
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                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 549(Y) 
                 −3621 
                 −2707 
                 −4176 
                 −4424 
                 2950 
                 −4049 
                 −394 
                 −2539 
                 −4002 
                 −1942 
                 −1987 
                 −2749 
                 −3933 
                 −2854 
                 −3451 
                 −3299 
                 −3499 
                 −2690 
                 349 
                 4094 
                 555 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
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                 359 
                 117 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 550(A) 
                 3438 
                 −1472 
                 −2846 
                 −3040 
                 −3287 
                 −1726 
                 −2735 
                 −2840 
                 −3028 
                 −3257 
                 −2662 
                 −2236 
                 −2447 
                 −2798 
                 −2944 
                 −1216 
                 −1387 
                 −2183 
                 −3405 
                 −3320 
                 556 
               
               
                 — 
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                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
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                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 551(H) 
                 −1741 
                 −2627 
                 −2070 
                 −1046 
                 −3303 
                 −2401 
                 2713 
                 −2751 
                 2478 
                 −2476 
                 −1755 
                 −1061 
                 −2375 
                 −27 
                 2379 
                 −1621 
                 −1497 
                 −2477 
                 −2379 
                 −2161 
                 557 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
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                 −249 
               
               
                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
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                 * 
                 * 
               
               
                 552(L) 
                 −1014 
                 −876 
                 −2956 
                 −2408 
                 −582 
                 −2550 
                 −1529 
                 1721 
                 −2079 
                 2042 
                 345 
                 −2114 
                 −2581 
                 −1775 
                 −2028 
                 454 
                 −980 
                 286 
                 −1414 
                 −1096 
                 558 
               
               
                 — 
                 −149 
                 −500 
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                 45 
                 96 
                 359 
                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 553(V) 
                 933 
                 −842 
                 −2818 
                 −2467 
                 −1542 
                 −1870 
                 −1890 
                 154 
                 −2226 
                 −1095 
                 −617 
                 −1932 
                 −2326 
                 −1995 
                 −2259 
                 −1126 
                 1070 
                 2769 
                 −2180 
                 −1826 
                 559 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
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                 117 
                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 554(S) 
                 −787 
                 −1522 
                 −1486 
                 −1172 
                 −2714 
                 −1599 
                 −1112 
                 −2500 
                 −433 
                 −2563 
                 −1791 
                 −1110 
                 −2067 
                 −796 
                 1351 
                 2916 
                 −989 
                 −1943 
                 −2648 
                 −2234 
                 560 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
                 96 
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                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 555(S) 
                 −326 
                 −1010 
                 −1779 
                 −1541 
                 −2691 
                 −1234 
                 −1566 
                 −2386 
                 −1486 
                 −2594 
                 −1749 
                 −1228 
                 1196 
                 −1330 
                 −1747 
                 2396 
                 1967 
                 −1662 
                 −2876 
                 −2496 
                 561 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
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                 275 
                 394 
                 45 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
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                 * 
                 * 
               
               
                 556(A) 
                 3121 
                 −934 
                 −2489 
                 −2561 
                 −3081 
                 −1203 
                 −2295 
                 −2766 
                 −2533 
                 −3080 
                 −2234 
                 −1669 
                 −1953 
                 −2234 
                 −2533 
                 936 
                 −746 
                 −1844 
                 −3331 
                 −3090 
                 562 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
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                 210 
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                 275 
                 394 
                 45 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 557(S) 
                 −897 
                 −1462 
                 −2333 
                 −2543 
                 −3185 
                 −1640 
                 −2474 
                 −3294 
                 −2686 
                 −3497 
                 −2780 
                 −1973 
                 −2360 
                 −2483 
                 −2703 
                 3465 
                 −1316 
                 −2413 
                 −3310 
                 −3025 
                 563 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
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                 275 
                 394 
                 45 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 558(R) 
                 −586 
                 −1873 
                 −516 
                 979 
                 −2188 
                 −1543 
                 −123 
                 −1869 
                 1290 
                 −353 
                 −980 
                 −202 
                 −1622 
                 314 
                 1886 
                 −491 
                 782 
                 −1495 
                 −2024 
                 −1439 
                 564 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 394 
                 45 
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                 — 
                 −16 
                 −7108 
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                 −894 
                 −1115 
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                 −1378 
                 * 
                 * 
               
               
                 559(G) 
                 −2594 
                 −2690 
                 −3304 
                 −3623 
                 −4328 
                 3747 
                 −3462 
                 −4761 
                 −3953 
                 −4671 
                 −4212 
                 −3320 
                 −3352 
                 −3748 
                 −3779 
                 −2839 
                 −2981 
                 −4004 
                 −3668 
                 −4222 
                 565 
               
               
                 — 
                 −149 
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                 233 
                 43 
                 −381 
                 399 
                 106 
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                 394 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 560(C) 
                 2804 
                 3772 
                 −3185 
                 −3198 
                 −2739 
                 −1303 
                 −2462 
                 −2065 
                 −2882 
                 −2628 
                 −1924 
                 −1927 
                 −2044 
                 −2547 
                 −2727 
                 −661 
                 −799 
                 −1463 
                 −3099 
                 −2886 
                 566 
               
               
                 — 
                 −149 
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                 233 
                 43 
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                 45 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
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                 −1378 
                 * 
                 * 
               
               
                 561(V) 
                 −1771 
                 −1603 
                 −3750 
                 −3689 
                 −2037 
                 −3050 
                 −3231 
                 403 
                 −3479 
                 −1154 
                 −1076 
                 −3246 
                 −3399 
                 −3383 
                 −3437 
                 −2628 
                 −1917 
                 3536 
                 −3074 
                 −2677 
                 567 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
                 394 
                 45 
                 96 
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                 −369 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 562(T) 
                 −1213 
                 −1674 
                 −2755 
                 −2906 
                 −3163 
                 −1922 
                 −2659 
                 −2698 
                 −2788 
                 −3105 
                 −2612 
                 −2311 
                 −2600 
                 −2708 
                 −2753 
                 −1463 
                 3819 
                 −2197 
                 −3286 
                 −3156 
                 568 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
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                 275 
                 394 
                 45 
                 96 
                 359 
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                 — 
                 −16 
                 −7108 
                 −8150 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 563(D) 
                 −2784 
                 −3432 
                 4016 
                 −1200 
                 −4140 
                 −2466 
                 −2197 
                 −4505 
                 −2621 
                 −4365 
                 −3956 
                 −1551 
                 −3014 
                 −2039 
                 −3232 
                 −2593 
                 −2938 
                 −4046 
                 −3710 
                 −3552 
                 569 
               
               
                 — 
                 −149 
                 −500 
                 233 
                 43 
                 −381 
                 399 
                 106 
                 −626 
                 210 
                 −466 
                 −720 
                 275 
                 394 
                 45 
                 96 
                 359 
                 117 
                 −369 
                 −294 
                 −249 
               
               
                 — 
                 −21 
                 −6715 
                 −7757 
                 −894 
                 −1115 
                 −701 
                 −1378 
                 * 
                 * 
               
               
                 564(F) 
                 −525 
                 −445 
                 −2202 
                 −1627 
                 1946 
                 −2001 
                 −744 
                 1247 
                 −1346 
                 952 
                 561 
                 1079 
                 −2030 
                 −1067 
                 −1362 
                 −1067 
                 −465 
                 338 
                 −714 
                 −230 
                 570 
               
               
                 — 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
               
               
                 — 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 * 
                 0