Patent Publication Number: US-2015083589-A1

Title: Bio sensing device

Description:
TECHNICAL FIELD 
     The present invention relates to a bio sensing device, and more particularly, to a bio sensing device having a biosensor and a measuring device which are integrated therein. 
     BACKGROUND ART 
     Biosensors are measuring instruments that examine the properties of a target substance using functions of an organism. These biosensors are excellent in sensitivity and reaction specificity because the biosensors use a biomaterial as a detecting element. The biosensors are divided into enzyme assay biosensors and immunoassay biosensors according to an analysis reaction, and into optical biosensors and electrochemical biosensors according to a method of quantitatively analyzing a target substance within a bio-sample. The enzyme assay biosensors are designed to use a specific reaction between an enzyme and a substrate and a specific reaction between an enzyme and an enzyme inhibitor, and the immunoassay biosensors are designed to a specific reaction between an antigen and an antibody. The optical biosensors are designed to measure a concentration of a target material by measuring transmittance, absorbance, or alteration in wavelength, and have been most generally used. 
     Generally, in order to check a value measured by a biosensor, the biosensor should be inserted into a measuring device. When the biosensor is inserted into the measuring device, the measuring device senses the insertion and analyzes a concentration of a target material according to an electrochemical method or the like. At this time, because of contact impedance caused by a connection node (for example, socket, connector or the like) to which the biosensor and the measuring device are connected, an error may occur in the result value measured by the biosensor. Furthermore, a sensing error may occur in the connection between the measuring device and the biosensor. For example, when measuring devices are produced in large quantities, a connection node of each measuring device may have a different impedance value. When an electrode or connector of the biosensor in an imperfect state is connected to the measuring device, the impedance may be varied. In such a state, it is impossible to guarantee the precision and reproducibility of measured values. 
     Furthermore, in the conventional bio sensing device where the biosensor is inserted into the measuring device, the biosensor, the measuring device, and a lancet device should be separately provided, which makes it inconvenient to carry and use the bio sensing device. 
     DISCLOSURE 
     Technical Problem 
     An embodiment of the present invention is directed to a bio sensing device capable of increasing precision and reproducibility of measured values. 
     Another embodiment of the present invention is directed to a bio sensing device which is easy to carry and use. 
     Technical Solution 
     In accordance with an embodiment of the present invention, a bio sensing device includes: a main substrate; a reaction part comprising a reaction reagent to react in an oxidation-reduction reaction with an analysis target material and a reaction electrode to generate an analogue electrical signal by causing the oxidation-reduction reaction, and located at an end of the main substrate; a signal processing part located on a first side of the main substrate and processing the analogue electrical signal; a screen display part displaying a processing result of the signal processing part; and a housing to house the main substrate. The screen display part is three-dimensionally disposed over the signal processing part, and a space for mounting a lancet is formed in the housing. 
     In accordance with another embodiment of the present invention, a bio sensing device comprising: a main substrate having a protruding end; a reaction part comprising a reaction reagent to react in an oxygen-reduction reaction with an analysis target material and a reaction electrode to generate an analogue electrical signal by causing the oxygen-reduction reaction, and located at the end of the main substrate; a signal processing part located on a first side of the main substrate and processing the analogue electrical signal; a screen display part displaying a processing result of the signal processing part. The screen display part is disposed on a second side of the main substrate and opposite to the first side, and a space for mounting a lancet is formed opposite to the end of the main substrate. 
     Advantageous Effects 
     According to the embodiments of the present invention, the bio sensing device has a structure in which a measuring device is electrically connected to a bio sensor without a connection node such as a connector or socket. Therefore, since there is no impedance caused by the connection between the reaction part and the measuring device through a connection node, signal distortion by contact impedance does not occur, which makes it possible to increase the precision of measurement. Furthermore, an electrode for detecting sensor insertion, which is required when the biosensor is connected to the measuring device, and an electrode for detecting the type of an analysis target material are not necessary. Therefore, a detection error does not occur. Accordingly, the bio sensing device may perform a bio-sending operation more reliably. Furthermore, since the biosensor and the lancet may be integrated with the measuring device, it is convenient to carry and use the bio sensing device. Furthermore, a complex switch is not used so as to be suitable for throwaway products, and a capillary tube is formed through the bracket. Therefore, it is possible to minimize the manufacturing cost. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a perspective view of a bio sensing device according to an embodiment of the present invention. 
         FIG. 2  is an exploded perspective view of  FIG. 1 . 
         FIG. 3  is a perspective view of main components of  FIG. 2 . 
         FIG. 4  is a perspective view illustrating a state where the main components of  FIG. 3  are assembled. 
         FIG. 5  is a side perspective view of  FIG. 4 . 
         FIGS. 6 and 7  are perspective views illustrating a state where a bracket and a reaction part of  FIG. 2  are coupled. 
         FIG. 8  is a detailed perspective view of the bracket of  FIG. 2 . 
         FIGS. 9 and 10  are perspective views illustrating a state that power supply is disconnected/connected. 
         FIGS. 11 to 13  are perspective views of bio sensing devices according to other embodiments of the present invention. 
         FIG. 14  is a perspective view of a bio sensing device according to another embodiment of the present invention. 
         FIGS. 15 to 24  are perspective views of bio sensing devices according to other embodiments of the present invention. 
     
    
    
     BEST MODE FOR THE INVENTION 
     Exemplary embodiments of the present invention will be described below in more detail with reference to the accompanying drawings. The present invention may, however, be embodied in different forms and should not be constructed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the present invention to those skilled in the art. In the exemplary embodiments of the present invention, a case in which a concentration of blood sugar in the blood is measured by ampero-metry will be taken as an example for descriptions. However, it may be easily understood by those skill in the art that the present invention may be applied to all electrochemical bio-sensing technologies including the blood sugar measurement. 
     Referring to  FIGS. 1 to 8 , a bio sensing device according to an embodiment of the present invention will be described. The bio sensing device according to the embodiment of the present invention includes a reaction part  1001 , a bracket  1002 , a reaction unit  1003 , a lancet mounting space  1004 , a lancet  1005 , a power supply breaker  1006 , a main substrate  1007 , a main substrate power pin  1008 , a battery  1009 , a battery terminal  1010 , a screen display part  1011 , an auxiliary substrate  1012 , a signal processing part  1013 , a temperature sensor  1014 , a voice/sound output part (speaker, buzzer or the like)  1015 , an upper housing  1016 , and a lower housing  1017 . As illustrated in the drawings, the bio sensing device according to the embodiment of the present invention is implemented by integrating a biosensor and a measuring device, and includes the lancet mounting space to attach/detach the lancet. 
     Referring to  FIG. 1 , a use example of the bio sensing device will be described as follows. First, a user pulls the power supply breaker  1006  out of the bio sensing device to change a power-off state to a power-on state. Then, the user takes the lancet  1005  out of the bio sensing device, and shoots a blood needle such that blood flows from his/her finger. Then, the user contacts a portion of the finger, from which blood flows, with the reaction part  1001 , and checks his/her blood sugar level through the screen display part  1011  after a predetermined time passes. The bio sensing device having been used for checking the blood sugar level is discarded by the user. 
     The bio sensing device according to the embodiment of the present invention has the following main features. First, the bracket  1002  and the reaction unit  1003  constitute the reaction part  1001  having a reaction chamber structure, thereby improving a sample introduction function through a capillary action. Second, the power supply breaker  100  having a simple structure may be used to disconnect/connect the power supply of the battery. Therefore, the battery power waste may be prevented when the bio sensing device is not used, and the manufacturing cost may be reduced. Third, as the lancet mounting space  1004  is formed in the bio sensing device, the biosensor, the measuring device, and the lancet  1005  may be integrated to thereby increase the use convenience. Fourth, the screen display part  1011  is disposed three-dimensionally with respect to the signal processing part  1013  on the main substrate  1007 , thereby preventing an increase in length and area of the bio sensing device by the lancet mounting space  1004 . 
     Hereafter, the components related to the above-described main features of the bio sensing device will be described in detail with reference to the accompanying drawings.  FIG. 2  is an exploded perspective view of  FIG. 1 .  FIG. 3  is a perspective view of main components of  FIG. 2 .  FIG. 4  is a perspective view illustrating a state where the main components of  FIG. 3  are assembled.  FIG. 5  is a side perspective view of  FIG. 4 . 
     The upper housing  1016  and the lower housing  1017  serve as a case of the bio sensing device. A left end of the lower housing  1017  has a support surface for fixing the reaction part  1001 , and a left end of the upper housing  1016  has a groove for exposure to the outside. 
     The signal processing part  1013  is disposed on the top surface of the main substrate  1007 , and the reaction part  1001  consisting of the bracket  1002  and the reaction unit  1003  is disposed on an end of the top surface of the main substrate  1007 . The reaction part  1013  formed on the end of the top surface of the main substrate  1007  includes a reaction electrode and a reaction reagent placed on the reaction electrode, and a conducting wire (not illustrated) for a signal transmission function is formed between the signal processing part  1013  and the reaction electrode of the reaction part  1003 . The reaction electrode is not shown in the drawing because it is covered by the reaction reagent. 
     Referring to  FIG. 5 , the screen display part  1011  is three-dimensionally disposed over the signal processing part  1013  of the main substrate  1007 . The screen display part  1011  is mounted on the top surface of the auxiliary substrate  1012 , and a data/power pin of the auxiliary substrate  1012  is connected to the main substrate  1007 . In this embodiment of the present invention, the screen display part  1011  and the auxiliary substrate  1012  are separately provided. However, it may be considered that the screen display part  1011  and the auxiliary substrate  1012  serve as one part for a display function. 
     The screen display part  1011  and the signal processing part  1013  may be disposed on the other surface of the main substrate  1007 . In this case, a separate auxiliary substrate for disposing the screen display part  1011  in a three-dimensional manner with respect to the signal processing part  1013  is not necessary. 
     The main substrate  1007  may include the temperature sensor  1014  to provide temperature information which is used to perform temperature compensation related to a blood sugar level measured through the reaction part  1001 . The temperature sensor  1014  may be disposed adjacent to the reaction part  1001  as illustrated in  FIG. 5 . The bio sensing device may include the voice/sound output part  1015  for diabetic patients who have a difficulty in perceiving a variety of information through the screen display part  1011  because of the failure of eyesight. The reaction part  1001  may be mounted on the main substrate  1007 , and the reaction part  1001  and the signal processing part  1013  of the main substrate  1007  may be electrically connected through the conducting wire so as to be integrated with each other. In particular, the reaction electrode of the reaction part  1001  may be electrically connected to the conducting wire through wire bonding so as to be integrated with each other. 
     The reaction part  1001  may be formed on a separate substrate instead of the main substrate  1007 . The separate substrate having the reaction part  1001  formed thereon may be electrically connected to the main substrate  1007  through a rigid/flexible printed circuit board bonding technology. Furthermore, the separate substrate having the reaction part  1001  formed thereon may be electrically connected to the main substrate  1007  through a heat seal so as to be integrated with each other. 
       FIG. 6  illustrates a state before the bracket  1002  and the reaction unit  1003  constituting the reaction part  1001  are coupled (that is, assembled into a product).  FIG. 7  illustrates a state after the bracket  1002  is coupled to the reaction part  1003 . That is, the bracket  1002  is slid and coupled to the front end of the reaction part  1003 . In this case, protrusions  1002   a  formed inside the bracket  1002  are caught in grooves  1007   a  formed in both sides of the main substrate  1007  under the reaction part  1003 , thereby fixing the bracket  1002 . 
       FIG. 8  is a detailed perspective view of the bracket  1002  of  FIG. 2 . As illustrated in  FIGS. 6 and 8 , the protrusions  1002   a  are formed inside the bracket  1002 . The top of the bracket  1002 , which is located over the reaction part  1003  according to the coupling, is closed, and the bottom of the bracket  1002 , which is located under the reaction part  1003  according to the coupling, is opened. The top surface of the bracket  1002  has an internally-curved shape  1002   b  at an end thereof. 
     When the bracket  1002  and the reaction part  1003  are coupled in such a manner, the top surface and inner side surfaces of the bracket  1002  and the top surface of the reaction part  1003  form a reaction chamber structure. That is, the top surface and inner side surfaces of the bracket  1002  and the top surface of the reaction part  1003  form a capillary tube to guarantee rapid sample introduction. The bracket  1002  functions as a protection lid, an air outlet, a space structure layer for a predetermined reaction space, a cover and a vent layer, which are capable of inducing a capillary action to suck a sample. 
     The internally-curved shape  1002   b  of the bracket  1002  may promote the capillary action such that a sample introduced from the end of the bracket  1002  is more quickly introduced to the reaction part  1003 . The curved shape  1002   b  also serves to guide with which portion of the reaction part  1001  the user should contact the sample. 
     The bracket  1002  may be formed of a plastic injection molding material, for example, a transparent PC material such that the user visually checks whether the sample is normally introduced to the reaction part  1003 . 
       FIG. 9  illustrates a state in which the power supply breaker  1006  is attached before the bio sensing device is used.  FIG. 10  illustrates a state in which the power supply breaker  1006  is detached when the bio sensing device is used. Referring to  FIG. 9 , before the bio sensing device is used, the power supply breaker  1006  is disposed between the main substrate power pin  1008  and the battery terminal  1010  such that the main substrate power pin  1008  and the battery terminal  1010  are not contacted with each other. Therefore, the power supply from the battery  1009  to the main substrate  1007  is disconnected. Referring to  FIG. 10 , when the power supply breaker  1006  is detached, the main substrate power pin  1008  and the battery terminal  1010  are contacted with each other. Then, power is supplied from the battery  1009  to the main substrate  1007 . 
     The power supply breaker  1006  is formed of a non-conductive flat film to disconnect the electrical connection between the main substrate power pin  1008  and the battery terminal  1010  which are formed of a metal. As such, when the power supply breaker  1006  is formed of a film, it is possible to achieve a cost reduction for the prevention of the battery power waste. 
     The lancet mounting space  1004  for mounting the lancet  1005  is formed in the right side of the upper and lower housings  1016  and  1017 . The upper and lower housings  1016  and  1017  have a groove formed at right ends thereof such that the user may easily attach/detach the lancet  1005 . 
       FIGS. 11 to 13  are perspective views of bio sensing devices according to other embodiments of the present invention.  FIG. 11  is a top perspective view of a bio sensing device including a reaction part having via holes A  132  formed therein.  FIG. 12  is a bottom perspective view of the bio sensing device of  FIG. 11 . Referring to  FIGS. 11 and 12 , the bio sensing device according to the embodiment of the present invention includes a screen display part  110 , a conducting wire  120 , via holes A  132 , via holes B  131 , a signal processing part  130 , an insulator layer  140 , a reaction electrode  151 , a reaction reagent layer  160 , a space layer  170 , and a cover  180 . 
     The insulator layer  140  may be formed of any materials as long as they can form a conductive electrode on an insulative substrate. Technologies related to the formation of a conductive electrode on an insulative substrate may include a method in which a conductive electrode is formed by a sputtering process using a shadow mask, a method in which an electrode is formed by a typical sputtering process and formed using a typical photolithography process or laser, and a method in which an electrode is formed using screen printing, electroless plating, or electrolyte plating. For example, the insulator layer  140  may be formed by the following process: 
     printing electronics are used to form a substrate using various materials such as film, glass, silicon, plastic, fiber and paper, and conductive ink, conductive paste or the like is used to form a pattern. Representative examples of the insulator layer  140  may include a printed circuit board (PBC) which is widely used in the field. Such an insulator layer may be applied the same to other embodiments of the present invention. 
     The reaction electrode  151 , the reaction reagent layer  160 , the space layer  170 , and the cover  180  constitute a reaction part. The reaction part is where a chemical reaction is caused by a provided material. A reaction chamber is implemented by the reaction reagent layer  160 , the space layer  170 , and the cover  180 . The reaction reagent layer  160  is where a material required for a predetermined reaction of the bio sensing device is disposed. 
     The space layer  170  surrounds the reaction reagent layer  160 . The cover  180  covers the reaction reagent layer  160 . A space formed by the space layer  170  and the cover  180  is where a capillary action occurs. The insulator layer  140  serves as a substrate where circuits are arranged. 
     The reaction electrode  151  serves to generate an electrical signal corresponding to a chemical reaction occurring in the reaction reagent layer. That is, the reaction electrode  151  generates an analogue signal corresponding to an oxidation-reduction reaction caused by a reaction reagent and an analysis target material (for example, blood sugar of blood). The reaction electrode  151  includes a working electrode and a reference electrode. Here, the reaction electrode  151  may include at least two electrodes for electrochemical measurement, and the number of electrodes may be set to three, five, eight or more. 
     The screen display part  110  serves to display a bio-sensing result. The screen display part  100  serves to inform a user of a measurement result value, and a voice/sound output unit (speaker, buzzer or the like) may be used instead of the screen display part. The screen display part  110  may include an LCD device, an electronic paper, or an LED device. 
     The signal processing part  130  includes a signal conversion unit, an arithmetic unit, and an output unit. The signal conversion unit is configured to convert an analogue signal, which is generated by the reaction electrode  151  and transmitted through the via holes A  132 , the conducting wire  120 , and the via holes B  131 , into a digital electrical signal. The arithmetic unit is configured to generate a measurement result value of the analysis target material from the digital electrical signal. The output unit is configured to display the generated measurement result value. That is, the signal processing part  130  receives an electrical signal for the reaction result in the reaction reagent layer, provided through the conducting wire  120 , and displays a result value corresponding to the electrical signal on the screen display part. The insulator layer  140  is where the conducting layer  120  is formed. 
     The conducting wire  120  serves to electrically connect the reaction part and the signal processing part, that is, transmit an analogue electrical signal (current or voltage) depending on an electrochemical reaction and an applied voltage between the reaction electrode  151  and the signal processing part  130 . The conducting wire  120  serves a signal transmission part in the embodiment of the present invention. 
     The operation process of the bio sensing device illustrated in  FIGS. 11 and 12  will be described as follows. In the following descriptions, a blood sugar measurement of ampero-metry will be taken as an example. 
     When the signal processing part  130  applies a voltage, the voltage is applied to the reaction electrode  151  through the via holes B  131 , the conducting wire  120 , and the via holes A  132 . As the voltage is applied to the reaction electrode  151 , an analogue signal (current) is generated according to an electrochemical reaction between a reaction reagent and an analysis target material (for example, blood sugar of blood) introduced on the reaction reagent. This current is transmitted from the reaction electrode  151  through the via holes A  132 , the conducting wire  120 , and the via holes B  131  to the signal processing part  130 . The signal processing part  130  derives a measurement result value (for example, a blood sugar level corresponding to a concentration of the target analysis material) corresponding to a current through arithmetic processing. The bio sensing device according to this embodiment of the present invention is characterized in that a signal transmission path is formed through the via holes  131  and  132  and thus the conducting wire  120  is disposed under the insulator layer. 
       FIG. 13  illustrates a structure having multiple substrates. Referring to  FIG. 13 , the conducting wire  120  may be formed on a second insulator layer  142 , or may be formed under a first insulator layer  141  and covered by the second insulator layer  142 . 
     In the structures illustrated in  FIGS. 11 to 13 , the via holes B  131  are formed so as not to change the configuration of the signal processing part, but may be modified in various manners.  FIGS. 11 to 13  illustrate that the conducting wire is disposed between the signal processing part and the via holes B  131 . However, only the via holes may be formed immediately in front of a pin terminal of the signal processing part without the conducting wire, or the pin terminal of the signal processing part may be drawn toward the bottom of the insulator layer without the conducting wire and the via holes B  131  such that the pin terminal and the conducting wire are directly connected to each other. 
       FIG. 14  is a perspective view of a bio sensing device according to another embodiment of the present invention. Referring to  FIG. 14 , the bio sensing device  100 A according to the embodiment of the present invention includes a screen display part  110 A, a conducting wire  120 A, a signal processing part  130 A, an insulator layer  140 A, a reaction electrode  151 A, a reaction reagent layer  160 A, a space layer  170 A, and a cover  180 A. The screen display part  110 A serves to display a bio-sensing result. Here, the reaction electrode  151 A, the reaction reagent layer  160 A, the space layer  170 A, and the cover  180 A constitute a reaction part. 
     The reaction part is where a chemical reaction is caused by a provided material. The reaction reagent layer  160 A, the space layer  170 A, and the cover  180 A form a reaction chamber, and the reaction reagent layer  160 A is where a material required for a predetermined reaction operation of the biosensor is disposed. The space layer  170 A surrounds the reaction reagent layer  160 . The cover  180 A covers the reaction reagent layer  160 A. A space formed by the spacer layer  170 A and the cover  180 A is where a capillary action may occur. The reaction electrode  151 A is configured to generate an electrical signal corresponding to a chemical reaction occurring in the reaction reagent layer  160 . The screen display part  110 A may include an LCD device or LED device, and any devices may be used as long as they display information on a screen. In particular, an electronic paper which has been recently developed may be used. The conducting wire  120 A serves to electrically connect the reaction electrode  151 A and the signal processing part  130 A. Here, the reaction electrode  151 A and the conducting wire  120 A are divided, but may be integrally patterned and formed depending on a manufacturing process of the bio sensing device. The signal processing part  130 A is configured to receive an electrical signal for a reaction result in the reaction reagent layer, provided through the conducting wire  120 A, and display a result value corresponding to the electrical signal on the screen display part. The insulator layer  140 A is where the conducting wire  120 A is formed. 
     In the conventional bio sensing device, the biosensor and the measuring device which are physically separated from each other should be connected to each other through a socket or the like to transmit an analogue electrical signal corresponding to an oxidation-reduction reaction occurring in the biosensor to the measuring device, in order to check the reaction state. During this process, an impedance value of the connection node may act as an obstacle to measuring the value of the analysis target material, thereby degrading the precision. In the bio sensing device according to the embodiment of the present, however, the reaction part and the signal processing part capable of processing the reaction result in the reaction part are formed on one substrate. Therefore, it is possible to exclude an impedance component caused by the connection node such as the socket through which the bio-sensor is physically connected to the measuring device in the conventional bio sensing device. Furthermore, an electrode for detection of sensor insertion and detection of an analysis target material, which has been required in the conventional biosensor, is not necessary in the embodiment of present invention. Therefore, it is possible to exclude an error caused by such an electrode. Furthermore, since contact implement is not formed, a signal generated by an electrochemical reaction in the reaction part may be more sensitively processed inside the bio sensing device. Therefore, the bio sensing device may perform the measurement with more precision. In this embodiment of the present invention, the reaction electrode of the reaction part and the signal processing part are electrically connected through the signal transmission part. Thus, it can be understood that the reaction electrode and the signal transmission part include a conductive material. 
     Furthermore, the bio sensing device according to the embodiment of the present invention may further include a memory unit (not illustrated) for storing identification information on the bio sensing device. The identification information may be displayed through the screen display part  110  or a display unit which is additionally provided. Here, the identification information may include at least one of the type of an analysis target material, a measurement condition, production information, user information and the like. For example, the bio sensing device may store and display the identification information so as to make it easy to identify for whom the bio sensing device is used among family members. 
       FIGS. 15 to 21  illustrate various embodiments of the bio sensing device according to the present invention. The following descriptions will be focused on specific features of the respective bio sensing devices according to the various embodiments of the present invention, and the bio sensing devices according to the various embodiments of the present invention may selectively include internal components of the above-described bio sensing devices. Furthermore, the respective features according to the various embodiments of the present invention may be combined in various manners so as to form a bio sensing device. 
       FIG. 15  is a perspective view of a bio sensing device according to another embodiment of the present invention. Referring to  FIG. 15 , the bio sensing device according to the embodiment of the present invention includes a screen display part  211 , a conducting wire  121 , a signal processing part  213 , an insulator layer  214 , reaction electrodes  221  and  222 , a reaction reagent layer  223 , a space layer  224 , and a cover  225 . 
     The bio sensing device according to the embodiment of the present invention includes a first substrate  210  and a second substrate  220 . The first substrate  210  may be implemented using the insulator layer illustrated in  FIG. 9 , and the second substrate  220  may be implemented using a flexible insulator layer including an electrode. The first and second substrates  210  and  220  may be formed of the same material. As a desirable example, the first substrate  210  may be implemented with a PCB or the like, and the second substrate  220  may be implemented with a flexible PCB or the like. The first substrate corresponds to the measuring device according to the embodiments of the present invention, and the second substrate corresponds to the reaction part according to the embodiments of the present invention. 
     The bio sensing device according to this embodiment of the present invention is characterized in that the first and second substrates  210  and  220  are bonded (refer to reference numeral  215 ) using a rigid/flexible PCB bonding technology which is widely used in the field. As the two substrates are used, it is possible to improve the reliability of the manufacturing process of the bio sensing device. More specifically, a substrate where the reaction reagent having a large effect on precision and reproducibility of a bio-sensing result is disposed may be separately manufactured, and a substrate where the signal processing part including electronic parts is disposed may be separately manufactured. Then, the two substrates may be bonded to thereby improve the reliability of the manufacturing process. 
       FIG. 16  is a perspective view of a bio sensing device according to another embodiment of the present invention. Referring to  FIG. 16 , the bio sensing device according to the embodiment of the present invention includes a screen display part  211 , a conducting wire  212 , a signal processing part  213 , an insulator layer  214 , reaction electrodes  321  and  322 , a reaction reagent layer  323 , a space layer  324 , and a cover  325 . The bio sensing device according to this embodiment of the present invention is characterized in that it includes first and second substrates  210  and  320  connected through a heat seal  310 . 
       FIG. 17  is a perspective view of a bio sensing device according to another embodiment of the present invention. Referring to  FIG. 17 , the bio sensing device  400  according to the embodiment of the present invention includes a screen display part  430 , a conducting wire  420 , a signal processing part  440 , an insulator layer  410 , reaction electrodes  461  and  462 , a reaction reagent layer  470 , a space layer  480 , and a cover  490 . The bio sensing device according to this embodiment of the present invention is characterized in that the reaction electrodes  461  and  462  are connected to the conducting wire  420  through wire bonding  450 . 
       FIG. 18  is a perspective view of a bio sensing device according to another embodiment of the present invention. Referring to  FIG. 18 , the bio sensing device according to the embodiment of the present invention includes a screen display part  510 , a conducting wire  550 , a signal processing part  520 , an insulator layer  530 , reaction electrodes  561  and  562 , a reaction reagent layer  570 , and a cover  590 . The bio sensing device according to this embodiment of the present invention is characterized in that the reaction electrodes  561  and  562  are connected to the conducting wire  550  through a conductive bump  540 . 
     As the conductive bump  540  is used, a capillary tube space is formed between the conductive bump  540  and the reaction electrodes  561  and  562 . Therefore, a space layer and a vent hole are not separately required. For example, since the reaction reagent layer  570  is connected to the conductive bump  540 , a separate vent hole is not necessary. Furthermore, when the conductive bump  540  is constructed in various shapes, the reaction reagent layer  570  may be inclined in such a manner as to introduce a sample more smoothly. Furthermore, as the conductive bump  540  is used, only an area which genuinely reacts may be efficiently made into the reaction reagent layer, which makes it possible to expect cost reduction and yield improvement.  FIG. 18  illustrates in the bottom that the conductive bump, the reaction electrode, and the reaction reagent layer are assembled. 
       FIG. 19  is a perspective view of a bio sensing device according to another embodiment of the present invention. Referring to  FIG. 19 , the bio sensing device according to the embodiment of the present invention includes a first substrate  610 , a second substrate  620 , a third substrate  630 , reaction electrodes  661  and  662 , a reaction reagent layer  670 , and a cover  690 . The bio sensing device according to this embodiment of the present invention is characterized in that it includes the plurality of substrates, and the respective substrates  610 ,  620 , and  630  may be used as insulator layers. An interconnection necessary for the bio sensing device may be formed in any one of the substrates  610 ,  620 , and  630 , or may be partially formed in the respective substrates  610 ,  620 , and  630 .  FIG. 19  does not illustrate other necessary parts, in order to emphasize that the bio sensing device uses the plurality of substrates. However, the bio sensing device may include various parts required for the above-described operation of the bio sensing device. 
       FIG. 20  is a perspective view of a bio sensing device according to another embodiment of the present invention. Referring to  FIG. 20 , the bio sensing device  600 A according to the embodiment of the present invention includes a first substrate  610 , a second substrate  620 , a third substrate  630 , reaction electrodes  661  and  662 , a reaction reagent layer  670 , a space layer  680 , and a cover  690 . The bio sensing device according to this embodiment of the present invention is characterized in that it includes the plurality of electrodes, and the respective substrates  610 ,  620 , and  630  may be used as insulator layers. That is, an interconnection necessary for the bio sensing device may be formed in any one of the substrates  610 ,  620 , and  630 , or may be partially formed in the respective substrates  610 ,  620 , and  630 . Furthermore, the bio sensing device according to the embodiment of the present invention includes the space layer  680  unlike the bio sensing device illustrated in  FIG. 19 . In the bio sensing device of  FIG. 19 , the cover  690  has a dome structure to function as the space layer. Therefore, the bio sensing device of  FIG. 19  does not need to be provided with a space layer. Such a cover structure may be applied to the other embodiments of the present invention. 
       FIG. 21  is a perspective view of a bio sensing device according to another embodiment of the present invention. Referring to  FIG. 21 , the bio sensing device according to the embodiment of the present invention includes a screen display part  700 , an integrated circuit (IC)  710 , a conducting wire  720 , reaction electrodes  761  and  762 , a reaction reagent layer  770 , a space layer  780 , and a cover  790 . In the bio sensing device according to the embodiment of the present invention, the IC  710  includes a signal processing part with active and passive elements, and is configured to contain a variety of information such as code information in the elements. That is, a separate memory unit for storing information is not additionally formed in the bio sensing device, and the IC  710  is implemented by containing code information in the signal processing part. For example, the IC  710  may be implemented with one IC chip which is typically called SoC (System on Chip). 
     Meanwhile, the configuration of the bio sensing device according to the embodiment of the present invention may be applied to the other embodiments of the present invention. Furthermore, as in the bio sensing device of  FIG. 15 , two substrates may be bonded (refer to reference numeral  730 ) using a rigid/flexible PCB bonding technology. 
     Hereafter, bio sensing devices including a plurality of reaction parts to perform various types of bio-sensing operations will be described with reference to  FIGS. 22 to 24 . 
       FIG. 22  is a perspective view of a bio sensing device including a plurality of reaction parts according to another embodiment of the present invention.  FIG. 23  is a perspective view of a bio sensing device including a plurality of reaction parts according to another embodiment of the present invention.  FIG. 24  is a perspective view of a bio sensing device including a plurality of reaction parts according to another embodiment of the present invention. 
     Referring to  FIGS. 22 and 23 , when the bio sensing device includes one screen display part (refer to reference numeral  81  of  FIG. 22  or reference numeral  97  of  FIG. 23 ) and a plurality of reaction parts provided in a signal processing part, the bio sensing device may perform various bio-sensing operations. Each of the reaction parts (refer to reference numeral  82  of  FIG. 22  or reference numerals  91 ,  92 , and  93  of  FIG. 23 ) includes reaction electrodes and a material for bio-sensing. Furthermore, the reaction part includes a plurality of conducting wires (signal transmission parts) for transmitting an electrical signal from the reaction electrodes to the signal processing part provided therein, and the number of the conducting wires corresponds to the number of the reaction parts. 
     The material for bio-sensing is provided in a reaction chamber disposed in the reaction part, and the reaction chamber may be applied according to the above-described various embodiments of the present invention. In particular, one substrate may be used, or a first substrate (refer to reference numeral  98  of  FIG. 23 ) and a plurality of second substrates (refer to reference numerals  91 ,  92 , and  93  of  FIG. 23 ) may be used. When one substrate is used, the reaction parts illustrated in  FIGS. 9 ,  17 ,  18 ,  19 , and  20  may be applied. When the first substrate and the second substrates are used, the reaction parts illustrated in  FIGS. 15 ,  16 , and  21  may be applied. Here, the substrate where the reaction part is located is referred to as an auxiliary substrate, and the substrate where the signal processing part is located is referred to as a main substrate. 
     Furthermore, when the bio sensing device is constructed as illustrated in  FIG. 22 , the plurality of reaction parts may be arranged only in one side, and when the bio sensing device is constructed as illustrated in  FIG. 23 , the plurality of reaction parts (refer to reference numerals  91 ,  92 , and  93 ) may be arranged in all directions. Furthermore, since the material for a chemical reaction is disposed in the reaction parts, the bio sensing device may further include a package for packaging the respective reaction parts (refer to reference numeral  95  of  FIG. 23 ) to perform a dampproof function. The bio sensing devices according to the above-described embodiments of the present invention may also include the package for a dampproof function, which has been described with reference to  FIG. 23 . Furthermore, the screen display part may include an LCD device, an LED device, or all kinds of display devices capable of displaying information. Furthermore, referring to  FIG. 24 , each reaction part  94  may include a pair of reaction electrode and chemical material, the reaction electrode having two or more electrodes (refer to a, b, c, and d). In this case, the reaction part may perform different kinds of bio-sensing operations. 
     In the above-described embodiments of the present invention, the detailed descriptions of additional components such as a power supply unit and a button were omitted, but a power supply unit (for example, battery, USB connection or the like) and a small number of input units (for example, key button, LCD screen menu and the like) may be further provided. 
     One or more of the features included in the above-described various embodiments of the present invention may be selected and combined to implement a bio sensing device, if necessary. 
     The above-described bio sensing devices according to the embodiments of the present invention may be applied to implement a bio sensing device capable of measuring blood sugar, glycosylated hemoglobin (HbAlc), and hemoglobin (Hb) which need to be measured together. 
     While the present invention has been described with respect to the specific embodiments, it will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the following claims.