Patent Publication Number: US-2023141052-A1

Title: Methods and compositions for genomic integration

Description:
CROSS REFERENCE 
     This application is a continuation application of the international application, PCT/US22/28831, filed on May 11, 2022; which claims the benefit of priority to U.S. Provisional Application No. 63/187,117, filed on May 11, 2021, U.S. Provisional Application No. 63/254,791, filed on Oct. 12, 2021, and U.S. Provisional Application No. 63/274,907, filed on Nov. 2, 2021, each of which is hereby incorporated by reference in its entirety. 
    
    
     SEQUENCE LISTING 
     The instant application contains a Sequence Listing which was submitted electronically in ASCII format in the parent international filing and is hereby incorporated by reference in its entirety. Said ASCII copy was created on Jun. 17, 2022, named 56371-723_601_SL.txt and was 677,993 bytes in size. Said file is currently updated in the ST26 format, renamed as 56371-723-301_SL_1-19-23.xml, saved as an XML file, and is 596,910 bytes in size, is filed concurrently; and is fully incorporated by reference. 
     BACKGROUND 
     Cell therapy is a rapidly developing field for addressing difficult to treat diseases, such as cancer, persistent infections and certain diseases that are refractory to other forms of treatment. Cell therapy often utilizes cells that are engineered ex vivo and administered to an organism to correct deficiencies within the body. An effective and reliable system for manipulation of a cell&#39;s genome is crucial, in the sense that when the engineered cell is administered into an organism, it functions optimally and with prolonged efficacy. Likewise, reliable mechanisms of genetic manipulation form the cornerstone in the success of gene therapy. However, severe deficiencies exist in methods for delivering nucleic acid cargo (e.g., large cargo) in a therapeutically safe and effective manner. Viral delivery mechanisms are frequently used to deliver large nucleic acid cargo in a cell but are tied to safety issues and cannot be used to express the cargo in some cell types. Additionally, subjecting a cell to repeated gene manipulation can affect cell health, induce alterations of cell cycle and render the cell unsuitable for therapeutic use. Advancements are continually sought in the area for efficacious delivery and stabilization of an exogenously introduced genetic material for therapeutic purposes. 
     SUMMARY 
     Provided herein is a pharmaceutical composition comprising a therapeutically effective amount of one or more polynucleic acids, or at least one vector encoding the one or more polynucleic acids, the one or more polynucleic acids comprising: a mobile genetic element comprising a sequence encoding a polypeptide; and an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous therapeutic polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into a genome of a cell; and wherein the pharmaceutical composition is substantially non-immunogenic to a human subject. 
     In some embodiments, the polypeptide encoded by the sequence of the mobile genetic element comprises one or more long interspersed nuclear element (LINE) polypeptides, wherein the one or more LINE polypeptides comprises: human ORF1p or a functional fragment thereof, and human ORF2p or a functional fragment thereof. 
     In some embodiments, the insert sequence stably integrates and/or is retrotransposed into the genome of a human cell. 
     In some embodiments, the human cell is an immune cell selected from the group consisting of a T cell, a B cell, a myeloid cell, a monocyte, a macrophage and a dendritic cell. 
     In some embodiments, the insert sequence is integrated into the genome (i) by cleavage of a DNA strand of a target site by an endonuclease encoded by the one or more polynucleic acids, (ii) via target-primed reverse transcription (TPRT) or (iii) via reverse splicing of the insert sequence into a DNA target site of the genome. In some embodiments, the insert sequence is integrated into the genome at a poly T site using specificity of an endonuclease domain of the human ORF2p. In some embodiments, the poly T site comprises the sequence TTTTTA. In some embodiments, the one or more polynucleic acids comprises homology arms complementary to a target site in the genome. In some embodiments, the insert sequence integrates into: (a) the genome at a locus that is not a ribosomal RNA locus; (b) a gene or regulatory region of a gene of the genome, thereby disrupting the gene or downregulating expression of the gene; (c) a gene or regulatory region of a gene of the genome, thereby upregulating expression of the gene; or (d) the genome and replaces a gene of the genome. In some embodiments, the pharmaceutical composition further comprises (i) one or more siRNAs and/or (ii) an RNA guide sequence or a polynucleic acid encoding the RNA guide sequence, and wherein the RNA guide sequence targets a DNA target site of the genome and the insert sequence is integrated into the genome at the DNA target site of the genome. In some embodiments, the one or more polynucleic acids have a total length of from 3 kb to 20 kb. In some embodiments, the one or more polynucleic acids comprises one or more polyribonucleic acids, one or more RNAs or one or more mRNAs. In some embodiments, the exogenous therapeutic polypeptide is selected from the group consisting of a ligand, an antibody, a receptor, an enzyme, a transport protein, a structural protein, a hormone, a contractile protein, a storage protein and a transcription factor. In some embodiments, the exogenous therapeutic polypeptide is a receptor selected from the group consisting of a chimeric antigen receptor (CAR) and a T cell receptor (TCR). In some embodiments, the one or more polynucleic acids comprises a first expression cassette comprising a promoter sequence, a 5′ UTR sequence, a 3′ UTR sequence and a poly A sequence; wherein: the promoter sequence is upstream of the 5′ UTR sequence, the 5′ UTR sequence is upstream of the sequence of the mobile genetic element encoding a polypeptide, the 3′ UTR sequence is downstream of the insert sequence; and the 3′ UTR is upstream of the poly A sequence; and wherein the 5′ UTR sequence, the 3′ UTR sequence or the poly A sequence comprises a binding site for a human ORF2p or a functional fragment thereof. In some embodiments, the insert sequence comprises a second expression cassette comprising a sequence that is a reverse complement of a promoter sequence, a sequence that is a reverse complement of a 5′ UTR sequence, a sequence that is a reverse complement of a 3′ UTR sequence and a sequence that is a reverse complement of a poly A sequence; wherein: (i) the sequence that is a reverse complement of a promoter sequence is downstream of the sequence that is a reverse complement of a 5′ UTR sequence, (ii) the sequence that is a reverse complement of a 5′ UTR sequence is downstream of the sequence that is a reverse complement of a sequence encoding an exogenous therapeutic polypeptide, (iii) the sequence that is a reverse complement of a 3′ UTR sequence is upstream of the sequence that is a reverse complement of a sequence encoding an exogenous therapeutic polypeptide, and (iv) the sequence that is a reverse complement of a poly A sequence is upstream of the sequence that is a reverse complement of a 3′ UTR sequence and downstream of the sequence of the mobile genetic encoding a polypeptide. In some embodiments, the promoter sequence of the first expression cassette is different from the promoter sequence of the second expression cassette. In some embodiments, the one or more LINE polypeptides comprises a first LINE polypeptide comprising the human ORF1p or functional fragment thereof and a second LINE polypeptide comprising the human ORF2p or functional fragment thereof, wherein the first LINE polypeptide and the second LINE polypeptide are translated from different open reading frames (ORFs). In some embodiments, the one or more polynucleic acids comprises a first polynucleic acid molecule encoding the human ORF1p or functional fragment thereof and a second polynucleic acid molecule encoding the human ORF2p or functional fragment thereof. In some embodiments, the one or more polynucleic acids comprises a 5′ UTR sequence and a 3′ UTR sequence, wherein the 5′ UTR comprises a 5′ UTR from LINE-1 or a sequence with at least 80% sequence identity to ACUCCUCCCCAUCCUCUCCCUCUGUCCCUCUGUCCCUCUGACCCUGCACUGUCCCAGC ACC (SEQ ID NO: 51); and/or the 3′ UTR comprises a 3′ UTR from LINE-1 or a sequence with at least 80% sequence identity to CAGGACACAGCCUUGGAUCAGGACAGAGACUUGGGGGCCAUCCUGCCCCUCCAACCC GACAUGUGUACCUCAGCUUUUUCCCUCACUUGCAUCAAUAAAGCUUCUGUGUUUGGA ACAG (SEQ ID NO: 52). In some embodiments, the sequence encoding the exogenous therapeutic polypeptide does not comprise introns. In some embodiments, the polypeptide encoded by the sequence of the mobile genetic element comprises a C-terminal nuclear localization signal (NLS), an N-terminal NLS or both. In some embodiments, the sequence encoding the exogenous polypeptide is not in frame with a sequence encoding the ORF1p or functional fragment thereof and/or is not in frame with a sequence encoding the ORF2p or functional fragment thereof. In some embodiments, the one or more polynucleic acids comprises a sequence encoding a nuclease domain, a nuclease domain that is not derived from ORF2p, a megaTAL nuclease domain, a TALEN domain, a Cas9 domain, a Cas6 domain, a Cas7 domain, a Cas8 domain, a zinc finger binding domain from an R2 retroelement, or a DNA binding domain that binds to repeat sequences. In some embodiments, the one or more polynucleic acids comprises a sequence encoding the nuclease domain, wherein the nuclease domain does not have nuclease activity or comprises a mutation that reduces activity of the nuclease domain compared to the nuclease domain without the mutation. In some embodiments, the ORF2p or functional fragment thereof lacks endonuclease activity or comprises a mutation selected from the group consisting of S228P and Y1180A, and/or wherein the ORF1p or functional fragment comprises a K3R mutation. In some embodiments, the insert sequence comprises a sequence that is a reverse complement of a sequence encoding two or more exogenous therapeutic polypeptides. In some embodiments, the one or more polynucleic acids comprises one or more polyribonucleic acids, wherein the exogenous therapeutic polypeptide is a receptor selected from the group consisting of a chimeric antigen receptor (CAR) and a T cell receptor (TCR), and wherein the pharmaceutical composition is formulated for systemic administration to a human subject. In some embodiments, the one or more polynucleic acids are formulated in a nanoparticle selected from the group consisting of (i) a lipid nanoparticle and a polymeric nanoparticle; and/or (ii) comprises one or more polynucleic acids selected from the group consisting of glycosylated RNAs, circular RNAs and self-replicating RNAs. 
     Provided herein is a method, wherein the method is: (i) a method of treating a disease or condition in a human subject in need thereof comprising administering a pharmaceutical composition described herein to the human subject; or (ii) a method of modifying a population of human cells ex vivo comprising contacting a composition to a population of human cell ex vivo, thereby forming an ex vivo modified population of human cells, the composition comprising one or more polynucleic acids, or at least one vector encoding the one or more polynucleic acids, the one or more polynucleic acids comprising: a mobile genetic element comprising a sequence encoding a polypeptide; and an insert sequence, wherein the insert sequence is a reverse complement of a sequence encoding an exogenous therapeutic polypeptide, wherein the ex vivo modified population of human cells is substantially non-immunogenic to a human subject. In some embodiments, the one or more polynucleic acids further comprise (i) a sequence encoding an integrase or a fragment thereof for site directed integration of the insert sequence into the genome and (ii) an integrase genomic landing site sequence that operable by the integrase, wherein the genome landing sequence is greater than 4 consecutive nucleotides long. In some embodiments, the ORF2 and the integrase are on separate polynucleotides. In some embodiments, the ORF2 and the integrase are on a single polynucleotide. In some embodiments, the integrase is not integrated into the genome of the cell. In some embodiments, the integrase is a mutated or truncated recombinant protein. In some embodiments, the integrase genomic landing sequence that is operable by the integrase is greater than 20 nucleotides long, or greater than 30 nucleotides long. In some embodiments, the insert sequence comprises an attachment site operable by the integrase. In some embodiments, the integrase genomic landing site is inserted into the genome using a guide RNA and a Cas system. In some embodiments, the guide RNA, the CAS system and the genomic landing sequence are in a polynucleotide that is separate from the polynucleotide comprising the sequence encoding the LINE1-ORFs and the insert sequence. In some embodiments, one or more ORF polypeptide sequence comprises a mutation. A method for a site-specific integration of a heterologous genomic insert sequence into the genome of a mammalian cell, the method comprising: (i) introducing into the cell (a) a polynucleotide comprising sequences encoding one or more human retrotransposon elements associated with the heterologous insert sequence, and (b) a polynucleotide comprising sequence encoding a guide RNA, an RNA guided integrase or a fragment thereof and a landing sequence operable by the integrase; (ii) verifying the integration of the heterologous insert sequence into the site of the genome. 
     Provided herein is a method for site-specific integration of a heterologous genomic insert using a LINE retrotransposon system, wherein the LINE retrotransposon system is modified to incorporate a fragment of an integrase protein that can recognize a genomic landing sequence of greater than 10 consecutive nucleotides long, and wherein the LINE retrotransposon system integrates the heterologous genomic insert into the genomic landing sequence recognized by the fragment of the integrase protein. In some embodiments, the method further comprises a step of incorporating into the genome the genomic landing sequence of greater than 4 consecutive nucleotides long. In some embodiments, the step of incorporating into the genome the genomic landing sequence is performed by an RNA-guided CRISPR-Cas system. In some embodiments, the RNA-guided CRISPR-Cas system has an editing function capable of incorporating a sequence of greater than 4 consecutive nucleotides long into a specific genome site. In some embodiments, the RNA-guided CRISPR-Cas system incorporates an ORF-mRNA binding sequence into a specified location within the genome that has sequence homology to the sequence of the guide RNA. In some embodiments, the insert is about 10 kilobases or greater than 10 kilobases. In some embodiments, the polynucleotide is mRNA. 
     Provided herein is a method of stably integrating an insert sequence into genomic DNA of a target cell, the method comprising: contacting a composition to the target cell, the composition comprising a polynucleic acid, wherein the polynucleic acid comprises: an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous polypeptide, and a mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA; stably integrating the insert sequence into the genomic DNA of the target cell; and expressing an exogenous polypeptide in the target cell, wherein the target cell is a human hepatocyte. In some embodiments, the human hepatocyte is a primary cell. In some embodiments, the human hepatocyte is a from a cultured hepatocyte cell line. In some embodiments, incorporating comprises electroporating under conditions optimum for a human hepatocyte. In some embodiments, the method further comprises culturing the human hepatocyte in vitro after incorporating for about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 8 hours, about 10 hours or about 24 hours. In some embodiments, the method further comprises introducing the human hepatocyte expressing the exogenous polypeptide into a human subject in need thereof. In some embodiments, at least 2% of the human hepatocytes express the exogenous polypeptide at day 10 after incorporating. 
     Provided herein is a method of stably integrating an insert sequence into genomic DNA of a target cell, the method comprising: contacting a composition to the target cell, the composition comprising a polynucleic acid, wherein the polynucleic acid comprises: an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous polypeptide, and a mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA; stably integrating the insert sequence into the genomic DNA of the target cell; and expressing an exogenous polypeptide in the target cell, wherein the target cell is a human cardiomyocyte. In some embodiments, the human cardiomyocyte is a primary cell. In some embodiments, the human cardiomyocyte is a from a cultured cardiomyocyte cell line. In some embodiments, incorporating comprises electroporating under conditions optimum for a human cardiomyocyte. In some embodiments, the method further comprises culturing the cardiomyocyte in vitro after incorporating for about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 8 hours, about 10 hours or up to 24 hours. In some embodiments, the method further comprises introducing the human cardiomyocyte expressing the exogenous polypeptide into a human subject in need thereof. In some embodiments, at least 2% of the human cardiomyocytes express the exogenous polypeptide at day 10 after incorporating. 
     Provided herein is a method of stably integrating an insert sequence into genomic DNA of a target cell, the method comprising contacting a composition to the target cell, the composition comprising a polynucleic acid, wherein the polynucleic acid comprises: an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous polypeptide, and a mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA; stably integrating the insert sequence into the genomic DNA of the target cell; and expressing an exogenous polypeptide in the target cell, wherein the target cell is a human retinal pigment epithelial cell. In some embodiments, the human retinal pigment epithelial cell is a primary cell. In some embodiments, the human retinal pigment epithelial is a from a cultured retinal pigment epithelial cell line. In some embodiments, incorporating comprises electroporating under conditions optimum for a human retinal pigment epithelial cell. In some embodiments, the method further comprises culturing the retinal pigment epithelial cell in vitro after incorporating for about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 8 hours, about 10 hours or up to 24 hours. In some embodiments, the method further comprises introducing the human retinal pigment epithelial cell expressing the exogenous polypeptide into a human subject in need thereof. In some embodiments, at least 2% of the human RPE express the exogenous polypeptide at day 10 after incorporating. 
     Provided herein is a method of stably integrating an insert sequence into genomic DNA of a target cell, the method comprising contacting a composition to the target cell, the composition comprising a polynucleic acid, wherein the polynucleic acid comprises: an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous polypeptide, and a mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA: stably integrating the insert sequence into the genomic DNA of the target cell; and expressing an exogenous polypeptide in the target cell, wherein the target cell is a human neuronal cell. In some embodiments, the human neuronal cell is a primary cell. In some embodiments, the human neuronal cell is a from a cultured neuronal cell line. In some embodiments, incorporating comprises electroporating under conditions optimum for a human neuronal cell. In some embodiments, the method further comprises culturing the neuronal cell in vitro after incorporating for about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 8 hours, about 10 hours or up to 24 hours. In some embodiments, the method further comprises introducing the human neuronal cell expressing the exogenous polypeptide into a human. In some embodiments, at least 2% of the human neuronal cells express the exogenous polypeptide at day 10 after incorporating. In some embodiments, the insert sequence is a human insert sequence. In some embodiments, the exogenous polypeptide is an exogenous therapeutic polypeptide. In some embodiments, the exogenous polypeptide is an exogenous human polypeptide. In some embodiments, the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA via target-primed reverse transcription (TPRT). In some embodiments, the polynucleic acid is an mRNA or an mRNA molecule. In some embodiments, the mobile genetic element comprises a human LINE 1 retrotransposon element. In some embodiments, the ORF2p is selected from a non-human species. In some embodiments, the ORF2p selected from a non-human species is further modified to enhance retrotransposition efficiency and/or translation efficiency. In some embodiments, the cell is an immune cell, a hepatocyte, a cardiomyocyte, a retinal pigment epithelial cell or a neuron. In some embodiments, the ORF2p comprises an nuclear localization sequence (NLS). In some embodiments, the ORF2p comprises at least 2 NLSs that are the same or different. In some embodiments, the NLS is N-terminal to a sequence encoding ORF1p, ORF2p or both. In some embodiments, the NLS is C-terminal to a sequence encoding ORF1p, ORF2p or both. In some embodiments, the NLS is from SV40. In some embodiments, the NLS is from nucleoplasmin. In some embodiments, a first NLS of the at least 2 NLSs is from SV40 and a second NLS of the at least 2 NLSs is from nucleoplasmin. In some embodiments, a first and a second NLS of the at least 2 NLSs are from SV40. In some embodiments, a first and a second NLS of the at least 2 NLSs are from nucleoplasmin. In some embodiments, each of the at least 2 NLSs are the same. 
     INCORPORATION BY REFERENCE 
     All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference. To the extent publications and patents or patent applications incorporated by reference contradict the disclosure contained in the specification, the specification is intended to supersede and/or take precedence over any such contradictory material. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings (also “FIG.” herein), of which: 
         FIG.  1 A  illustrates a general mechanism of action of retrotransposons. (I) is a schematic representing the overall lifecycle of an autonomous retrotransposon. (II) LINE-1 retrotransposon comprises LINE-1 elements, which encode two proteins ORF1p and ORF2p that are expressed as mRNAs. The bicistronic mRNA is translated into the two proteins, and when ORF2p is translated by a read-through event by the ribosome, it binds the 3′ end of its own mRNA through the poly A tail (III). ORF2p cleaves at a consensus sequence TAAAA, where the poly A at the 3′ end of the mRNA hybridizes and primes the reverse transcriptase activity of the ORF2 protein. The protein reverse-transcribes the mRNA back into DNA leading to an insertion of the LINE-1 sequence back into a new location in the genome (IV). 
         FIG.  1 B  is an illustration of a schematic diagram of an mRNA construct that comprises a genetic payload (left) that can be designed for integration into the genome (right). 
         FIG.  1 C  illustrates various exemplary designs for integrating an mRNA encoding a transgene into the genome of a cell. GFP shown here in a box is an exemplary transgene. 
         FIG.  1 D  illustrates various exemplary designs for integrating an mRNA encoding a transgene into the genome of a cell. GFP shown here in a box is an exemplary transgene. 
         FIG.  1 E  is an illustration a schematic of the LINE-1 retrotransposition cycle showing the mechanism of action of the LINE transposons and introduction of a transgene cargo into a retrotransposon cite. LINE-1 retrotransposons are genomic sequences that encode for two proteins, ORF1 and ORF2. These elements are transcribed and translated into proteins that form an RNA-protein complex with the LINE-1 mRNA, ORF1 trimers, and ORF2, a reverse-transcriptase endonuclease. This complex translocates back into the nuclease where it cleaves DNA at a 5′-TTTT N-3′ motif and is primed for reverse-transcription of the LINE-1 RNA by the ORF2 protein by making an RNA-DNA hybrid with the poly A tail of the mRNA and the resected cleaved DNA. Reverse-transcription of the LINE-1 into cDNA leads to a new LINE-1 integration event. 
         FIG.  2 A  illustrates three exemplary designs for expressing an exemplary transgene GFP by stably incorporating the sequence encoding GFP using the constructs. Expected GFP expression levels at 72 hours are shown on the right side. 
         FIG.  2 B  illustrates three exemplary designs for expressing an exemplary transgene GFP by stably incorporating the sequence encoding RFP, RFP and GFP or ORF2p and GFP using the constructs. Expected GFP and RFP expression levels at 72 hours are shown on the right side. 
         FIG.  3 A  illustrates an exemplary diagram of conventional circRNA structure and formation. 
         FIG.  3 B  illustrates two views of an exemplary RL-GAAA tectoRNA motif designs.  FIG.  3 B  discloses SEQ ID NOS 111-112, respectively, in order of appearance. 
         FIG.  3 C  illustrates exemplary structures of chip-flow piece RNAs as platforms for testing potential tectoRNA. 
         FIG.  4 A  illustrates an exemplary schematic showing ORF2p binding to an ORF2 poly A region. 
         FIG.  4 B  illustrates an exemplary schematic showing how a fusion of ORF2p with an MS2 RNA binding domain binds to an MS2 binding RNA sequence in the 3′UTR of an mRNA encoding the ORF2 an increase specificity. 
         FIG.  4 C  illustrates exemplary designs of retrotransposon systems for stably integrating a nucleic acid into the genome of a cell at specific sites. The upper panel shows a design using an ORFp2-MegaTAL DNA binding domain fusion where the DNA binding and endonuclease activity of ORF2p is mutated to be inactive. The middle panel shows a chimeric ORF2p where the endonuclease domain has been replaced with a high specificity and high-fidelity nuclease domain of another protein. The lower panel shows a fusion of a DNA binding domain of a heterologous protein with ORF2p such that the fusion protein binds to ORF2 binding site as well additional DNA sequences in the vicinity of the ORF2 site. 
         FIG.  5    illustrates exemplary constructs (I)-(X) for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  6 A  illustrates an exemplary construct with a sequence encoding ORF1p for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  6 B  illustrates an exemplary construct without a sequence encoding ORF1p for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  7 A  illustrates exemplary methods of improving mRNA half-life by inhibiting degradation by 5′-3′ exonucleases, such as XRN1, or 3′-5′ exosomal degradation, by introducing structures corresponding to a G-quadruplex, or, a pseudoknot (SEQ ID NO: 113) in the 5′UTR; and/or xrRNAs, a triplex motifs (SEQ ID NOS: 74, 54, and 53, respectively, in order of appearance) and/or a non-A nucleotide residues in the 3′UTR. 
         FIG.  7 B  illustrates an exemplary schematic of a myeloid cell expressing a transgene encoding a chimeric receptor that binds a cancer cell and induces anti-cancer activity. 
         FIG.  7 C  shows expected results of introducing bulk or purified RNA encoding a chimeric receptor that binds a cancer cell as described in  FIG.  7 B  on increased and prolonged expression of the chimeric receptors. 
         FIG.  8 A  shows an exemplary plasmid design and expected LINE-1 mRNA transcript with a cargo nucleic acid sequence. The plasmid has a LINE-1 sequence (comprising ORF1 and ORF2 protein encoding sequences) and a cargo sequence which is a nucleic acid sequence encoding GFP, where the coding sequence of GFP is interrupted with an intron. The GFP is not expressed until the sequence is integrated in the genome and the intron is spliced. 
         FIG.  8 B  shows exemplary results showing successful integration of the mRNA transcript encoded by the plasmid shown in  FIG.  8 A  and expression of GFP relative to mock-transfected cells (fold increase in mean fluorescence intensity of GFP positive cells is shown). Mock transfected cells were transfected by the vector lacking the GFP cargo sequence. 
         FIG.  8 C  shows exemplary flow cytometry results from the results shown in  FIG.  8 B . 
         FIG.  9 A  shows an exemplary plasmid design and expected LINE-1 mRNA transcript with a cargo nucleic acid sequence. The plasmid has a LINE-1 sequence (comprising ORF1 and ORF2 protein encoding sequences) and a cargo sequence which is a nucleic acid sequence encoding a recombinant chimeric fusion receptor protein (ATAK receptor) that has extracellular region capable of binding to CD5 and an intracellular region comprising an FCR intracellular domain and a PI3 kinase recruitment domain. The coding sequence of the ATAK receptor is interrupted with an intron. 
         FIG.  9 B  shows exemplary results showing successful integration of the mRNA transcript encoded by the plasmid shown in  FIG.  9 A  and expression of ATAK relative to mock-transfected cells (fold increase in mean fluorescence intensity of ATAK positive cells is shown). Mock transfected cells were transfected by the vector lacking the ATAK cargo sequence. Expression of ATAK receptor protein was detected by binding with a labeled CD5 antibody. 
         FIG.  9 C  shows exemplary flow cytometry results from the results shown in  FIG.  9 B . 
         FIG.  10 A  shows an exemplary plasmid design and expected LINE-1 mRNA transcript with a cargo nucleic acid sequence. The plasmid has a LINE-1 sequence (comprising ORF1 and ORF2 protein encoding sequences) and a cargo sequence which is a nucleic acid sequence encoding a recombinant chimeric fusion receptor protein (ATAK receptor) followed by a T2A self-cleavage sequence followed by a split GFP sequence (all in a reverse orientation relative to the LINE-1 sequence). The coding sequence of the GFP is interrupted with an intron. Expected mRNA after reverse transcription and integration of the cargo are depicted. 
         FIG.  10 B  shows exemplary results showing successful integration of the mRNA transcript encoded by the plasmid shown in  FIG.  10 A  and expression of ATAK-T2A-GFP relative to mock-transfected cells (fold change in GFP and ATAK double positive cells is shown). Mock transfected cells were transfected by the vector lacking the ATAK cargo sequence. Expression of ATAK receptor protein was detected by binding with a labeled CD5 antibody. 
         FIG.  10 C  shows representative flow cytometry data from two separate experimental runs for expression of both GFP and CD5 binder (ATAK) using the experimental setup shown in  FIG.  10 A . 
         FIG.  10 D  shows representative flow cytometry data from two separate experimental runs for expression of both GFP and CD5 binder (ATAK) using the experimental setup shown in  FIG.  10 A . 
         FIG.  11 A  shows exemplary mRNA constructs for retrotransposition-based gene delivery. The ORF1 and ORF2 sequences are in two difference mRNA molecules. The ORF2p (ORF2) coding mRNA comprises and inverted GFP coding sequence. 
         FIG.  11 B  depicts exemplary data showing expression of GFP (fold increase in mean fluorescence intensity of GFP positive cells is shown) upon electroporating both ORF1-mRNA and ORF2-FLAG-GFPai mRNA normalized to electroporation of ORF2-FLAG-GFPai mRNA only. 
         FIG.  12 A  depicts exemplary data showing expression of GFP (fold increase in mean fluorescence intensity of GFP positive cells is shown) upon electroporating ORF1-mRNA and ORF2-FLAG-GFPai mRNA at different amounts. Fold increase is relative to 1×ORF2-GFPao and 1×ORF1 mRNA. 
         FIG.  12 B  shows an exemplary fluorescent microscopy image of GFP+ cells following electroporation of the mRNA depicted in  FIG.  11 A . 
         FIG.  13 A  shows exemplary mRNA constructs where the ORF1 and ORF2 sequences are in two difference mRNA molecules (top panel) and a LINE-1 mRNA transcript comprising ORF1 and ORF2 protein encoding sequences on a single mRNA molecule (bottom panel) for gene delivery. mRNA contains the bicistronic ORF1 and ORF2 sequence with a CMV-GFP sequence in the 3′UTR going from 3′-5′. Upon retrotransposition of the delivered ORF2-cmv-GFP antisense (LINE-1 mRNA), cells are expected to express GFP. 
         FIG.  13 B  depicts exemplary data showing expression of GFP (fold increase in mean fluorescence intensity of GFP positive cells is shown) upon electroporating the constructs depicted in  FIG.  13 A . 
         FIG.  14 A  shows an exemplary experimental design for testing whether multiple electroporations increases retrotransposition efficiency. HEK293T cells were electroporated every 48 hours with the Maxcyte system and assessed for GFP positive cells using flow after culturing for 24-72 hrs. 
         FIG.  14 B  depicts exemplary data showing expression of GFP at the indicated times (fold increase in mean fluorescence intensity of GFP positive cells is shown) upon electroporating 1-5 times according to  FIG.  14 A . 
         FIG.  15 A  depicts exemplary constructs to enhance retrotransposition via mRNA delivery. In one construct a nuclear localization signal (NLS) sequence is fused to the C terminus of the ORF2 sequence (ORF2-NLS fusion). In one construct a Minke whale ORF2 sequence was used in place of the human ORF2. In one construct a minimal sequence of the Alu element (AJL-H33delta) is inserted in the 3′UTR of the LINE-1 sequence. In one construct MS2 hairpins are inserted in the 3′UTR of the LINE-1 sequence and an MS2 hairpin binding protein (MCP) sequence is fused to the ORF2 sequence. 
         FIG.  15 B  depicts exemplary data showing expression of GFP (fold increase in mean fluorescence intensity of GFP positive cells is shown) using the constructs depicted in  FIG.  15 A . 
         FIG.  16 A  shows exemplary plasmid constructs where the ORF1 and ORF2 sequences are in two difference plasmid molecules (top panel) and a plasmid encoding a LINE-1 mRNA transcript comprising ORF1 and ORF2 protein encoding sequences on a single mRNA molecule with various replacements of the inter-ORF sequence between ORF1 and ORF2 (bottom panel) for gene delivery. 
         FIG.  16 B  depicts exemplary data showing expression of GFP (fold increase in mean fluorescence intensity of GFP positive cells is shown) using the constructs depicted in  FIG.  16 A . 
         FIG.  17 A  depicts an exemplary plasmid construct encoding a LINE-1 mRNA transcript comprising ORF1 and ORF2 protein encoding sequences on a single mRNA molecule with a GFP sequence (top panel) and an exemplary LINE-1 mRNA transcript comprising ORF1 and ORF2 protein encoding sequences on a single mRNA molecule with a GFP sequence. 
         FIG.  17 B  depicts exemplary data showing expression of GFP (fold increase in mean fluorescence intensity of GFP positive cells is shown) in Jurkat cells using the constructs depicted in  FIG.  17 A . The plasmid construct was transfected, and the mRNA construct was electroporated. 
         FIG.  18 A  shows an exemplary plasmid design and expected LINE-1 mRNA transcript with a cargo nucleic acid sequence. The plasmid has a LINE-1 sequence (comprising ORF1 and ORF2 protein encoding sequences) and a cargo sequence which is a nucleic acid sequence encoding a recombinant chimeric fusion receptor protein (ATAK receptor) followed by a T2A self-cleavage sequence followed by a split GFP sequence (all in a reverse orientation relative to the LINE-1 sequence). The coding sequence of the GFP is interrupted with an intron. Expected mRNA after reverse transcription and integration of the cargo are depicted. 
         FIG.  18 B  shows exemplary results showing successful integration of the mRNA transcript encoded by the plasmid shown in  FIG.  10 A  and expression of ATAK-T2A-GFP relative to mock-transfected cells (fold change in GFP and ATAK double positive cells is shown) in a myeloid cell line (THP-1). Data represents expression at 6 days post transfection, normalized over mock plasmid transfected cells wherein the mock plasmid does not have GFP coding sequence. 
         FIG.  19    illustrates an exemplary experimental set up for cell synchronization. A heterogenous cell population is sorted based on cell cycle stage, prior to delivery of an exogenous nucleic acid. Cell cycle synchronization is expected to result in higher expression and stabilization of the exogenous nucleic acid delivered. If cells are not homogeneous after cell sorting, then cells can be further incubated with a suitable agent that arrests cell cycle at a stage. 
         FIG.  20    illustrates an exemplary method for increasing retrotransposon efficiency by inducing DNA double stranded breaks, with or without inhibiting DNA repair pathways, such as by inducing DNA ligase inhibitor SCR7 or inhibiting host surveillance proteins, for example, using miRNA to HUSH complex TASOR protein. 
         FIG.  21    illustrates exemplary constructs for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  22    illustrates exemplary constructs for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  23    illustrates exemplary constructs for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  24    illustrates exemplary constructs for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  25    illustrates exemplary constructs for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  26    illustrates exemplary constructs for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  27    illustrates exemplary constructs for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  28    illustrates exemplary constructs for integrating an mRNA encoding a transgene into the genome of a cell. 
         FIG.  29    illustrates exemplary retrotransposon constructs (left) with a 2.4 kb cargo with a general mechanism of action of the retrotransposon, and a representative data (right) for expression of a fluorescent GFP marker encoded by the cargo from a nucleic acid sequence integrated into the genome in HEK293 cells. Placement of an antisense GFP gene split with an intron in the sense direction and a promoter sequence in the 3′UTR of the LINE-1 leads to reconstitution and retrotransposition of the GFP cargo. GFP expression in 293T cells transfected with the construct shown on the left, as measured by flow cytometry (right) and quantitated bar graphs (bottom left). Data collected 35 days after doxycycline induction of the ORF. 
         FIG.  30    illustrates exemplary retrotransposon constructs (left) with a 3.0 kb cargo comprising a membrane protein (CD5 binder chimeric antigen receptor, CD5-CAR), and a representative flow cytometry data for expression of the CD5 binder (right) from the nucleic acid sequence integrated into the genome in HEK293 cells. % of CD5 binder positive (+) cells is indicated in the inset. 
         FIG.  31    illustrates an exemplary retrotransposon construct (top) with a 3.7 kb cargo comprising a membrane protein (CD5 binder chimeric antigen receptor, CD5-CAR and a GFP separated by an auto-cleavable T2A element), and a representative flow cytometry data (bottom) demonstrating the expression of the CD5 binder and GFP. 
         FIG.  32    illustrates an exemplary retrotransposon construct (top) with a 3.9 kb cargo comprising a membrane protein (HER2 binder chimeric antigen receptor, and a GFP separated by an auto-cleavable T2A element), and a representative flow cytometry data (bottom) demonstrating the expression of the HER2 binder and GFP. 
         FIG.  33 A  shows exemplary data for delivery of retrotransposon elements delivered as mRNA. 
         FIG.  33 B  shows schematic diagram showing a trans and a cis mRNA design for delivery of LINE1 mRNA with GFP cargo (top panel). Representative results of electroporation of 293T cells with trans mRNAs with separate ORF1 and ORF2 mRNAs. 293T cells were electroporated with 100 ug/mL of mRNA either with ORF2 alone, ORF1+ORF2 mRNAs, each at 100 ug/mL, or a GFP-encoding mRNA with the same 5′ and 3′UTRs as the ORF1 mRNA (left panel of data plots). Retrotransposition events result in GFP-positive cells. Cells were assayed for GFP fluorescence by flow cytometry 4 days and 10 days post-electroporation. Mock electroporated cells serve as the negative control population for gating. Bar graph on the right shows results from a representative experiment indicating titration of trans mRNAs and cis ORF1 and ORF2 containing mRNA concentration during electroporation. Trans mRNAs solid bars and cis mRNA stripes. 20× is 2000 ug/mL in the electroporation reaction. 
         FIG.  33 C  shows titration of the ORF1 and ORF2-GFPai trans mRNAs. Increasing the concentration separately and together during the electroporation to 200 ug/mL increases retrotransposition of the GFP gene cargo. 
         FIG.  33 D  illustrates an exemplary data for the different constructs indicated above each flow cytometry data plot in the figure, the top panel on day 4, and the bottom panel on day 13. Right hand figures illustrate light and fluorescent microscopic images of a the GFP expressing cells in culture. Copies of integrated cargo per construct is demonstrated in the bottom right at day 13. qPCR assay for genomic DNA integration from different LINE-1 plasmid transfected, LINE-1 mRNA (retro-mRNA), and ORF1 and ORF2-GFP mRNA electroporated cells is shown. Two qPCR primer-probe sets were used, one for the housekeeping gene RPS30 and the other for the GFP gene. Plasmid-transfected cells use a plasmid that does not contain and SV40 maintenance sequence. Integration per cell is calculated from determining copy numbers per samples through interpolation of a standard curve of plasmid and genomic DNA, and normalizing for the two copies of RPS30 per 293T cell. Error bars denote standard deviation of three technical replicate measurements. 
         FIG.  34    illustrates exemplary retrotransposon construct (left) and expression data (right) in the indicated cell lines. 
         FIG.  35    illustrates flow cytometry data showing expression of LINE 1 GFP constructs in K562, 293T and THP1 cells (upper panel); and number of integrations of LINE-2-GFP mRNA per cell in K562 and THP-1 cell lines (lower panel). 
         FIG.  36    illustrates flow cytometry data showing expression of LINE 1 GFP constructs in primary T cells (left). Integrations per cell are indicated in the graph on the right. Data was collected on day 6 after electroporation. 
         FIG.  37 A  shows a schematic of activation, culture times, electroporation, and GFP expression assay of isolated primary T cells. 
         FIG.  37 B  illustrates flow cytometry data showing expression of LINE 1 GFP mRNA constructs in primary T cells at the indicated concentrations and before and after freeze-thaw as indicated in the figure. Integrations per cell is shown in the bar diagram. GFP expression using a retro-mRNA electroporation with a GFP cargo. GFP expression was assayed 4 days post electroporation and 15 days of culturing post electroporation. Primary T cells were cryo-preserved and thawed during this time. qPCR integration assay for GFP integration. Genomic DNA from the 20×sample was isolated and assayed for copies of GFP. 
         FIG.  38    demonstrates a summary of results of retrotransposon integration and expression across cell types. 
         FIG.  39    shows various applications of the technology described herein, including but not limited to use of CART cells, NK cells, neurons and other cells for cell therapy, and use of in vivo applications in including but not limited to gene therapy, gene editing, transcription regulation, and genome engineering. 
         FIG.  40    depicts exemplary flow cytometry data showing sorting and enriching GFP+ 293T cells electroporated with 2000 ng/μL LINE1-GFP mRNA. The first panel shows flow cytometry data for mock electroporated cells in the absence of LINE1-GFP mRNA. The second panel shows flow cytometry data collected 5 days post electroporation for unsorted cells electroporated with LINE 1-GFP mRNA. The GFP+ cells from the second panel were sorted and the flow cytometry data are shown in the third panel. The GFP+ cells from the third panel were cultured for 9 days post sorting and resorted using 10{circumflex over ( )}3 or 10{circumflex over ( )}4 GFP fluorescence intensity gate. The fourth panel shows flow cytometry data for cells resorted using GFP+ at 10{circumflex over ( )}3 GFP gate collected 4 days after resorting. The fifth panel shows flow cytometry data for cells resorted using GFP+ at 10{circumflex over ( )}3 GFP gate collected 4 days after resorting. 
         FIG.  41 A  shows a standard curve for GFP (NB2 plasmid) and a housekeeping gene (FAU) for evaluating genomic integration of GFP-encoding nucleic acid per cell using quantitative PCR. 
         FIG.  41 B  shows results of an exemplary graph depicting interpolation of the standard curves of  FIG.  41 A  for quantitation of genomic integration. 
         FIG.  41 C  shows the number of the GFP gene integrated into genome of 293T cells following LINE 1-GFP mRNA electroporation and double sorting as shown in  FIG.  40   . The average number of GFP integrations per cell when gated at 10{circumflex over ( )}3 GFP+ cells and at 10{circumflex over ( )}4 GFP+ cells according to qPCR are shown. 
         FIG.  42    depicts exemplary flow cytometry data showing GFP+ 293T cells electroporated with the indicated titrated amounts of LINE 1-GFP mRNA, in ng/μL in electroporation solution, after culturing for 3 days post-electroporation. 
         FIG.  43    depicts exemplary flow cytometry data showing GFP+ 293T cells electroporated with the indicated titrated amounts of LINE 1-GFP mRNA, in ng/μL in electroporation solution, after culturing for 5 days post-electroporation. 
         FIG.  44    depicts exemplary flow cytometry data showing GFP+ 293T cells electroporated with the indicated titrated amounts of LINE 1-GFP mRNA, in ng/μL in electroporation solution, after culturing for 7 days post-electroporation. 
         FIG.  45    shows a graph of the number of GFP integrations per genome of 293T cells electroporated with the indicated titrated amounts of LINE1-GFP mRNA, in ng/μL in electroporation solution, according to qPCR after culturing for 3, 5 or 7 days post-electroporation according to  FIGS.  42 - 44    (top) and a graph of the integration kinetics (bottom) according to the data from  FIGS.  42 - 44   . 
         FIG.  46    depicts exemplary flow cytometry data (right) showing GFP+ K562 cells electroporated with the indicated titrated amounts of LINE1-GFP mRNA, in ng/μL in electroporation solution, after culturing for 6 days post-electroporation, and a graph of the number of GFP integrations per genome according to qPCR (left). 
         FIG.  47    depicts exemplary flow cytometry data (top) showing GFP+ human primary monocytes electroporated with the indicated titrated amounts of LINE1-GFP mRNA after culturing for 3 days post-electroporation, and a graph of the number of GFP integrations per genome according to qPCR (bottom). 
         FIG.  48    depicts exemplary flow cytometry data (bottom) showing GFP+ 293T cells electroporated with 2000 ng/μL LINE1-GFP mRNA and 100 ng/μL, 200 ng/μL or 300 ng/μL of an siRNA targeting BRCA1 (siBRCA1) after culturing for 4 days post-electroporation and a graph of the number of GFP integrations per genome according to qPCR (top). 
         FIG.  49    depicts exemplary flow cytometry data (bottom) showing GFP+ 293T cells electroporated with 2000 ng/μL LINE1-GFP mRNA and 100 ng/μL of an siRNA targeting RNASEL (siRNASEL), ADAR1 (siADAR1), or ADAR2 (siADAR2) after culturing for 6 days post-electroporation and a graph of the number of GFP integrations per genome according to qPCR (top). 
         FIG.  50    depicts exemplary flow cytometry data (bottom) showing GFP+ 293T cells electroporated with 2000 ng/μL LINE1-GFP mRNA and 100 ng/μL of an siRNA targeting APOBEC3C (siAPOBEC3C) or FAM208A (siFAM208A) after culturing for 6 days post-electroporation and a graph of the number of GFP integrations per genome according to qPCR (top). 
         FIG.  51    depicts exemplary flow cytometry data (bottom) showing GFP+ 293T cells electroporated with 1000 ng/μL or 1500 ng/μL LINE 1-GFP mRNA and an siRNA cocktail with 25 ng/μL, 50 ng/μL or 75 ng/μL of each siRNA targeting RNASEL (siRNASEL), ADAR1 (siADAR1), ADAR2 (siADAR2) and BRCA1 (siBRCA1) after culturing for 6 days post-electroporation and a graph of the number of GFP integrations per genome according to qPCR (top). 
         FIG.  52    depicts exemplary flow cytometry data (bottom) showing GFP+ K562 cells electroporated with 1000 ng/μL LINE1-GFP mRNA and an siRNA cocktail with 25 ng/μL, 50 ng/μL or 75 ng/μL of each siRNA targeting RNASEL (siRNASEL), ADAR1 (siADAR1), ADAR2 (siADAR2) and BRCA1 (siBRCA1) after culturing for 5 days post-electroporation and a graph of the number of GFP integrations per cell according to qPCR (top). 
         FIG.  53    depicts a schematic showing exemplary locations of extraneous nuclear localization sequences (NLS) and exemplary ORF1p and ORF2p mutations of an exemplary LINE1-GFP mRNA construct. 
         FIG.  54 A  depicts a schematic showing an exemplary LINE1-GFP construct in which an NLS was inserted at the N-terminal end of the sequence encoding ORF1. 
         FIG.  54 B  depicts a bar graph showing GFP integrations per cell on day 4 post electroporation of the indicated constructs into 293T cells. 
         FIG.  54 C  depicts exemplary flow cytometry showing GFP+ 293T cells on day 4 post electroporation of the indicated constructs. 
         FIG.  55 A  depicts a schematic showing an exemplary LINE1-GFP construct in which an NLS was inserted at the C-terminal end of the sequence encoding ORF1. 
         FIG.  55 B  depicts a bar graph showing GFP integrations per cell on day 4 post electroporation of the indicated constructs into 293T cells. 
         FIG.  55 C  depicts exemplary flow cytometry showing GFP+ 293T cells on day 4 post electroporation of the indicated constructs. 
         FIG.  56 A  depicts a schematic showing an exemplary LINE1-GFP construct in which an NLS was inserted at the N-terminal end of the sequence encoding ORF2. 
         FIG.  56 B  depicts a bar graph showing GFP integrations per cell on day 4 post electroporation of the indicated constructs into 293T cells. 
         FIG.  56 C  depicts exemplary flow cytometry showing GFP+ 293T cells on day 4 post electroporation of the indicated constructs. 
         FIG.  57 A  depicts a schematic showing an exemplary LINE 1-GFP construct in which an NLS and a linker was inserted at the N-terminal end of the sequence encoding ORF2. 
         FIG.  57 B  depicts a bar graph showing GFP integrations per cell on day 5 post electroporation of the indicated constructs into 293T cells. 
         FIG.  57 C  depicts exemplary flow cytometry showing GFP+ 293T cells on day 5 post electroporation of the indicated constructs. 
         FIG.  58 A  depicts a schematic showing an exemplary LINE1-GFP construct in which an NLS was inserted at the C-terminal end of the sequence encoding ORF2. 
         FIG.  58 B  depicts a bar graph showing GFP integrations per cell on day 5 post electroporation of the indicated constructs into 293T cells. 
         FIG.  58 C  depicts exemplary flow cytometry showing GFP+ 293T cells on day 5 post electroporation of the indicated constructs. 
     
    
    
     DETAILED DESCRIPTION 
     The present invention arises in part from the exciting discovery that a polynucleotide could be designed and developed to accomplish transfer and integration of a genetic cargo (e.g., large genetic cargo) into the genome of a cell. In some embodiments, the polynucleotide comprises (i) a genetic material for stable expression, and (ii) a self-integrating genomic integration machinery that allows stable integration of the genetic material into a cell by non-viral means, that is both safe and efficacious. Moreover, the genetic material may be integrated at a locus other than a ribosomal locus; the genetic material may be integrated site-specifically; and/or the integrated genetic material appear to express without triggering a cell&#39;s natural silencing machinery. 
     Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR) revolutionized the molecular biology field and has developed into a potent gene editing too. It utilizes homology-directed repair (HDR) and can be directed to a genomic site. CRISPR/Cas9 is a naturally occurring RNA-guided endonuclease. While the CRISPR/Cas9 system has demonstrated great promise for site-specific gene editing and other applications, there are several factors that influence its efficacy which must be addressed, especially if it is to be used for in vivo human gene therapy. These factors include target DNA site selection, sgRNA design, off-target cutting, incidence/efficiency of HDR vs. NHEJ, Cas9 activity, and the method of delivery. Delivery remains the major obstacle for use of CRISPR for in vivo applications. Zinc finger nucleases ZFNs are a fusion protein of Cys2-His2 zinc finger proteins (ZFPs) and a non-specific DNA restriction enzyme derived from FokI endonucleases. Challenges with ZFPs include design and engineering of the ZFP for high-affinity binding of the desired sequence, which is non-trivial. Also, not all sequences are available for ZFP binding, so site selection is limited. Another significant challenge is off-target cutting. Transcription activator-like effector nucleases (TALENs) are a fusion protein comprised of a TALE and a FokI nuclease. While off-target cutting remains a concern, TALENs have been shown in one side-by-side comparison study to be more specific and less cytotoxic than ZFNs. However, TALENs are substantially larger, and the cDNA encoding TALEN only is 3 kb. This makes delivery of a pair of TALENs more challenging than a pair of ZFNs due to delivery vehicle cargo size limitations. Further, packaging and delivery of TALENs in some viral vectors may be problematic due to the high level of repetition in the TALENs sequence. A mutant Cas9 system, a fusion protein of inactive dCas9 and a FokI nuclease dimer increase specificity and reduce off-target cutting, the number of potential target sites is lower due to PAM and other sgRNA design constraints. 
     The present invention addresses the problems described above by providing new, effective and efficient compositions comprising transposon-based vectors for providing therapy, including gene therapy, to animals and humans. The present invention provides methods of using these compositions for providing therapy to animals and humans. These transposon-based vectors can be used in the preparation of a medicament useful for providing a desired effect to a recipient following administration. Gene therapy includes, but is not limited to, introduction of a gene, such as an exogenous gene, into an animal using a transposon-based vector. These genes may serve a variety of functions in the recipient such as coding for the production of nucleic acids, for example RNA, or coding for the production of proteins and peptides. The present invention can facilitate efficient incorporation of the polynucleotide sequences, including the genes of interest, promoters, insertion sequences, poly A and any regulatory sequences. The invention is based on the finding that human LINE-1 elements are capable of retrotransposition in human cells as well as cells of other animal species and can be manipulated in a versatile manner to achieve efficient delivery and integration of a genetic cargo into the genome of a cell. Such LINE-1 elements have a variety of uses in human and animal genetics including, but not limited to, uses in diagnosis and treatment of genetic disorders and in cancer. The LINE-1 elements of the invention are also useful for the treatment of various phenotypic effects of various diseases. For example, LINE-1 elements may be used for transfer of DNA encoding anti-tumorigenic gene products into cancer cells. Other uses of the LINE-1 elements of the invention will become apparent to the skilled artisan upon a reading of the present specification. 
     In general, a human LINE-1 element comprises a 5′ UTR with an internal promoter, two non-overlapping reading frames (ORF1 and ORF2), a 200 bp 3′ UTR and a 3′ poly A tail. The LINE-1 retrotransposon can also comprise an endonuclease domain at the LINE-1 ORF2 N-terminus. The finding that LINE-1 encodes an endonuclease demonstrates that the element is capable of autonomous retrotransposition. LINE-1 is a modular protein that contains non-overlapping functional domains which mediate its reverse transcription and integration. In some embodiments, the sequence specificity of the LINE-1 endonuclease itself can be altered or the LINE-1 endonuclease can be replaced with another site-specific endonuclease. 
     The LINE-1 retrotransposon may be manipulated using recombinant technology to comprise and/or be contiguous with, other nucleic acid elements which render the retrotransposon suitable for insertion of substantial lengths (up to 1 kb, or greater than 1 kb, e.g. greater than 5, 6, 7, 8, 9, or 10 kb) of heterologous or homologous nucleic acid sequence into the genome of a cell. The LINE-1 retrotransposon may also be manipulated using the same type of technology such that insertion of the nucleic acid sequence of heterologous or homologous nucleic acid into the genome of a cell is site-directed (site into which such DNA is inserted is known). Alternatively, the LINE-1 retrotransposon may be manipulated such that the insertion site of the DNA is random. The retrotransposon may also be manipulated to effect insertion of a desired DNA sequence into regions of DNA which are normally transcriptionally silent, wherein the DNA sequence is expressed in a manner such that it does not disrupt the normal expression of genes in the cell. In some embodiments, the integration or retrotransposition is in the trans orientation. In some embodiments, the integration or retrotransposition occurs in the cis orientation. 
     Since LINE-1 is native to human cells, when the constructs are placed into human cells, they should not be rejected by the immune system as foreign. In addition, the mechanism of LINE-1 retro-integration ensures that only one copy of the gene is integrated at any specific chromosomal location. Accordingly, there is a copy number control built into the system. In contrast, gene transfer procedures using ordinary plasmids offer little or no control regarding copy number and often result in complex arrays of DNA molecules tandemly integrated into the same genomic location. 
     All terms are intended to be understood as they would be understood by a person skilled in the art. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosure pertains. 
     The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described. 
     As used herein, the singular forms “a”, “an” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. 
     In this application, the use of “or” means “and/or” unless stated otherwise. The terms “and/or” and “any combination thereof” and their grammatical equivalents as used herein, may be used interchangeably. These terms may convey that any combination is specifically contemplated. Solely for illustrative purposes, the following phrases “A, B, and/or C” or “A, B, C, or any combination thereof” may mean “A individually; B individually; C individually; A and B; B and C; A and C; and A, B, and C.” The term “or” may be used conjunctively or disjunctively unless the context specifically refers to a disjunctive use. 
     The term “about” or “approximately” may mean within an acceptable error range for the particular value as determined by one of ordinary skill in the art, which will depend in part on how the value is measured or determined, i.e., the limitations of the measurement system. For example, “about” may mean within 1 or more than 1 standard deviation, per the practice in the art. Alternatively, “about” may mean a range of up to 20%, up to 10%, up to 5%, or up to 1% of a given value. Alternatively, particularly with respect to biological systems or processes, the term may mean within an order of magnitude, within 5-fold, and more preferably within 2-fold, of a value. Where particular values are described in the application and claims, unless otherwise stated the term “about” meaning within an acceptable error range for the particular value should be assumed. 
     As used in this specification and claim(s), the words “comprising” (and any form of comprising, such as “comprise” and “comprises”), “having” (and any form of having, such as “have” and “has”), “including” (and any form of including, such as “includes” and “include”) or “containing” (and any form of containing, such as “contains” and “contain”) are inclusive or open-ended and do not exclude additional, unrecited elements or method steps. It is contemplated that any embodiment discussed in this specification may be implemented with respect to any method or composition of the present disclosure, and vice versa. Furthermore, compositions of the present disclosure may be used to achieve methods of the present disclosure. 
     Reference in the specification to “some embodiments,” “an embodiment,” “one embodiment” or “other embodiments” means that a particular feature, structure, or characteristic described in connection with the embodiments is included in at least some embodiments, but not necessarily all embodiments, of the present disclosures. To facilitate an understanding of the present disclosure, a number of terms and phrases are defined below. 
     Although various features of the present disclosure can be described in the context of a single embodiment, the features can also be provided separately or in any suitable combination. Conversely, although the present disclosure can be described herein in the context of separate embodiments for clarity, the disclosure can also be implemented in a single embodiment. 
     Applications of the present disclosure encompasses, but are not limited to methods and compositions related to expression of an exogenous nucleic acid in a cell. In some embodiments, the exogenous nucleic acid is configured for stable integration in the genome of a cell, such as a myeloid cell. In some embodiments, the stable integration of the exogenous nucleic acid may be at specific targets within the genome. In some embodiments, the exogenous nucleic acid comprises one or more coding sequences. In some embodiments, the exogenous nucleic acid may comprise one or more coding comprising a nucleic acid sequence encoding an immune receptor. In some embodiments, the present disclosure provides methods and compositions for a stable incorporation of a nucleic acid encoding a transmembrane receptor implicated in an immune response function (e.g. a phagocytic receptor or synthetic chimeric antigen receptor) into human macrophage or dendritic cell or a suitable myeloid cell or a myeloid precursor cell. An exogenous nucleic acid can refer to a nucleic acid that was not originally in a cell and is added from outside the cell, irrespective of whether it comprises a sequence that may already be present in the cell endogenously. An exogenous nucleic acid may be a DNA or an RNA molecule. An exogenous nucleic acid may comprise a sequence encoding a transgene. An exogenous nucleic acid may encode a recombinant protein, such as a recombinant receptor, or a chimeric antigen receptor (CAR). An exogenous nucleic acid may be referred to as a “genetic cargo” in the context of the exogenous nucleic acid being delivered inside a cell. The genetic cargo may be a DNA or an RNA. Genetic material can generally be delivered inside a cell ex vivo by a few different known techniques using either chemical (CaCl 2 -medicated transfection), or physical (electroporation), or biological (e.g. viral infection or transduction) means. 
     Provided herein are compositions and methods for stable, non-viral transfer and integration of genetic material into a cell. In one aspect, the genetic material is a self-integrating polynucleotide. The genetic material can be stably integrated in the genome of the cell. The cell may be a human cell. The method is designed for a safe and reliable integration of a genetic material into the genome of a cell. 
     Provided herein is pharmaceutical composition comprising a therapeutically effective amount of one or more polynucleic acids, or at least one vector encoding the one or more polynucleic acids, the one or more polynucleic acids comprising: (a) a mobile genetic element comprising a sequence encoding a polypeptide; and (b) an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous therapeutic polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into a genome of a cell; and wherein the pharmaceutical composition is substantially non-immunogenic to a human subject. 
     In some embodiments, the polypeptide encoded by the sequence of the mobile genetic element comprises one or more long interspersed nuclear element (LINE) polypeptides, wherein the one or more LINE polypeptides comprises: (i) human ORF1p or a functional fragment thereof, and (ii) human ORF2p or a functional fragment thereof. 
     In some embodiments, the insert sequence stably integrates and/or is retrotransposed into the genome of a human cell. 
     In some embodiments, the human cell is an immune cell selected from the group consisting of a T cell, a B cell, a myeloid cell, a monocyte, a macrophage and a dendritic cell. 
     In some embodiments, the insert sequence is integrated into the genome (i) by cleavage of a DNA strand of a target site by an endonuclease encoded by the one or more polynucleic acids, (ii) via target-primed reverse transcription (TPRT) or (iii) via reverse splicing of the insert sequence into a DNA target site of the genome. 
     In some embodiments, the insert sequence is integrated into the genome at a poly T site using specificity of an endonuclease domain of the human ORF2p. 
     In some embodiments, the poly T site comprises the sequence TTTTTA. 
     In some embodiments, the one or more polynucleic acids comprises homology arms complementary to a target site in the genome. 
     In some embodiments, the insert sequence integrates into: (a) the genome at a locus that is not a ribosomal locus; (b) a gene or regulatory region of a gene of the genome, thereby disrupting the gene or downregulating expression of the gene; (c) a gene or regulatory region of a gene of the genome, thereby upregulating expression of the gene; or (d) the genome and replaces a gene of the genome. 
     In some embodiments, the pharmaceutical composition further comprises (i) one or more siRNAs and/or (ii) an RNA guide sequence or a polynucleic acid encoding the RNA guide sequence, and wherein the RNA guide sequence targets a DNA target site of the genome and the insert sequence is integrated into the genome at the DNA target site of the genome. 
     In some embodiments, one or more genes are knocked down in the methods provided herein. In some embodiments, one or more siRNAs are employed in the compositions or methods described herein. For example, one or more genes can be knocked down to enhance integration, such as through modulating a pathway that may inhibit LINE-1. In some embodiments, the one or more genes knocked down include ADAR1, ADAR2 (ADAR1B), APOBEC3C, BRCA1, let-7 miRNA, RNase L, TASHOR (HUSH complex) and/or RAD51. For example, knock down of RNase L can be used to enhance integration by inhibiting or preventing degradation of an mRNA, such as an mRNA transcribed from a LINE-1. For example, knock down of ADAR1, ADAR2 (ADAR1B), and/or BRCA1 can be used to enhance integration by inhibiting or preventing ADAR1, ADAR2 (ADAR1B), and/or BRCA1 from inhibiting the cis binding of ORF2p to a poly A tail for L1 RNP assembly. For example, knock down of let-7 miRNA can be used to enhance integration by inhibiting or preventing let-7 miRNA from inhibiting translation, such as translation of ORF2p. let-7 miRNA. For example, knock down of RAD51 and/or BRCA1 can be used to enhance integration by inhibiting or preventing repair of cleaved DNA by RAD51 and/or BRCA1. 
     In some embodiments, the one or more polynucleic acids have a total length of from 3 kb to 20 kb. 
     In some embodiments, the one or more polynucleic acids comprises one or more polyribonucleic acids, one or more RNAs or one or more mRNAs. 
     In some embodiments, the exogenous therapeutic polypeptide is selected from the group consisting of a ligand, an antibody, a receptor, an enzyme, a transport protein, a structural protein, a hormone, a contractile protein, a storage protein and a transcription factor. 
     In some embodiments, the exogenous therapeutic polypeptide is a receptor selected from the group consisting of a chimeric antigen receptor (CAR) and a T cell receptor (TCR). 
     In some embodiments, the one or more polynucleic acids comprises a first expression cassette comprising a promoter sequence, a 5′ UTR sequence, a 3′ UTR sequence and a poly A sequence; wherein: (i) the promoter sequence is upstream of the 5′ UTR sequence, (ii) the 5′ UTR sequence is upstream of the sequence of the mobile genetic element encoding a polypeptide, (iii) the 3′ UTR sequence is downstream of the insert sequence; and (iv) the 3′ UTR is upstream of the poly A sequence; and wherein the 5′ UTR sequence, the 3′ UTR sequence or the poly A sequence comprises a binding site for a human ORF2p or a functional fragment thereof. 
     In some embodiments, the insert sequence comprises a second expression cassette comprising a sequence that is a reverse complement of a promoter sequence, a sequence that is a reverse complement of a 5′ UTR sequence, a sequence that is a reverse complement of a 3′ UTR sequence and a sequence that is a reverse complement of a poly A sequence; wherein: (i) the sequence that is a reverse complement of a promoter sequence is downstream of the sequence that is a reverse complement of a 5′ UTR sequence, (ii) the sequence that is a reverse complement of a 5′ UTR sequence is downstream of the sequence that is a reverse complement of a sequence encoding an exogenous therapeutic polypeptide (iii) the sequence that is a reverse complement of a 3′ UTR sequence is upstream of the sequence that is a reverse complement of a sequence encoding an exogenous therapeutic polypeptide, and (iv) the sequence that is a reverse complement of a poly A sequence is upstream of the sequence that is a reverse complement of a 3′ UTR sequence and downstream of the sequence of the mobile genetic encoding a polypeptide. 
     In some embodiments, the promoter sequence of the first expression cassette is different from the promoter sequence of the second expression cassette. 
     In some embodiments, the one or more LINE polypeptides comprises a first LINE polypeptide comprising the human ORF1p or functional fragment thereof and a second LINE polypeptide comprising the human ORF2p or functional fragment thereof, wherein the first LINE polypeptide and the second LINE polypeptide are translated from different open reading frames (ORFs). 
     In some embodiments, the one or more polynucleic acids comprises a first polynucleic acid molecule encoding the human ORF1p or functional fragment thereof and a second polynucleic acid molecule encoding the human ORF2p or functional fragment thereof. 
     In some embodiments, the one or more polynucleic acids comprises a 5′ UTR sequence and a 3′ UTR sequence, wherein (a) the 5′ UTR comprises a 5′ UTR from LINE-1 or a sequence with at least 80% sequence identity to ACUCCUCCCCAUCCUCUCCCUCUGUCCCUCUGUCCCUCUGACCCUGCACUGUCCCAGCACC (SEQ ID NO: 51); and/or (b) the 3′ UTR comprises a 3′ UTR from LINE-1 or a sequence with at least 80% sequence identity to 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 52) 
               
               
                   
                 CAGGACACAGCCUUGGAUCAGGACAGAGACUUGGG 
               
               
                   
                   
               
               
                   
                 GGCCAUCCUGCCCCUCCAACCCGACAUGUGUACCU 
               
               
                   
                   
               
               
                   
                 CAGCUUUUUCCCUCACUUGCAUCAAUAAAGCUUCU 
               
               
                   
                   
               
               
                   
                 GUGUUUGGAACAG. 
               
            
           
         
       
     
     In some embodiments, the sequence encoding the exogenous therapeutic polypeptide does not comprise introns. 
     In some embodiments, the polypeptide encoded by the sequence of the mobile genetic element comprises a C-terminal nuclear localization signal (NLS), an N-terminal NLS or both. 
     In some embodiments, the sequence encoding the exogenous polypeptide is not in frame with a sequence encoding the ORF1p or functional fragment thereof and/or is not in frame with a sequence encoding the ORF2p or functional fragment thereof. 
     In some embodiments, the one or more polynucleic acids comprises a sequence encoding a nuclease domain, a nuclease domain that is not derived from ORF2p, a megaTAL nuclease domain, a TALEN domain, a Cas9 domain, a Cas6 domain, a Cas7 domain, a Cas8 domain, a zinc finger binding domain from an R2 retroelement, or a DNA binding domain that binds to repeat sequences. 
     In some embodiments, the one or more polynucleic acids comprises a sequence encoding the nuclease domain, wherein the nuclease domain does not have nuclease activity or comprises a mutation that reduces activity of the nuclease domain compared to the nuclease domain without the mutation. 
     In some embodiments, the ORF2p or functional fragment thereof lacks endonuclease activity or comprises a mutation selected from the group consisting of S228P and Y1180A, and/or wherein the ORF1p or functional fragment comprises a K3R mutation. 
     In some embodiments, the insert sequence comprises a sequence that is a reverse complement of a sequence encoding two or more exogenous therapeutic polypeptides. 
     In some embodiments, the one or more polynucleic acids comprises one or more polyribonucleic acids, wherein the exogenous therapeutic polypeptide is a receptor selected from the group consisting of a chimeric antigen receptor (CAR) and a T cell receptor (TCR), and wherein the pharmaceutical composition is formulated for systemic administration to a human subject. 
     In some embodiments, the one or more polynucleic acids (i) are formulated in a nanoparticle selected from the group consisting of a lipid nanoparticle and a polymeric nanoparticle; and/or (ii) comprises one or more polynucleic acids selected from the group consisting of glycosylated RNAs, circular RNAs and self-replicating RNAs. 
     Also provided herein is a method of treating a disease or condition in a human subject in need thereof comprising administering a pharmaceutical composition described herein to the human subject. 
     Also provided herein is a method of modifying a population of human cells ex vivo comprising contacting a composition to a population of human cell ex vivo, thereby forming an ex vivo modified population of human cells, the composition comprising one or more polynucleic acids, or at least one vector encoding the one or more polynucleic acids, the one or more polynucleic acids comprising: (a) a mobile genetic element comprising a sequence encoding a polypeptide; and (b) an insert sequence, wherein the insert sequence is a reverse complement of a sequence encoding an exogenous therapeutic polypeptide, wherein the ex vivo modified population of human cells is substantially non-immunogenic to a human subject. 
     In one aspect, provided herein are compositions and methods that allow integration of genetic material into the genome of a cell, wherein the genetic material that can be integrated is not specifically restricted by size. In some aspects, the method described herein provides a one-step, single polynucleotide-mediated delivery and integration of genetic “cargo” in the genome of a cell. The genetic material may comprise a coding sequence, e.g., a sequence encoding a transgene, a peptide, a recombinant protein, or an antibody or fragments thereof, wherein the method and compositions ensure stable expression of the transcribed product encoded by the coding sequence. The genetic material may comprise a non-coding sequence, for example, a regulatory RNA sequences, e.g., a regulatory small inhibitory RNA (siRNA), microRNA (miRNA), long non-coding RNA (lncRNA), or one or more transcription regulators such as a promoter and/or an enhancer, and may also include, but not limited to structural biomolecules such as ribosomal RNA (rRNA), transfer RNA (tRNA) or a fragment thereof or a combination thereof. 
     In another aspect, provided herein are methods and compositions for site-specific integration of a genetic material that may not be specifically restricted by size, into the genome of a cell via a non-viral delivery that ensures both safety and efficacy of the transfer. Provided methods and compositions may be particularly useful in developing a therapeutic, such as a therapeutic comprising a polynucleotide comprising a genetic material and a machinery that allows transfer into a cell and stable integration into the genome of the cell into which the polynucleotide or an mRNA encoding the polynucleotide is transferred. In some embodiments, the therapeutic may be a cell that comprises a polynucleotide that has been stably integrated into the genome of the cell using the methods and compositions described herein. 
     In one aspect, the present disclosure provides compositions and methods for stable gene transfer into a cell. In some embodiments, the compositions and methods are for stable gene transfer into an immune cell. In some cases, the immune cell is a myeloid cell. In some cases, the methods described herein relate to development of myeloid cells for immunotherapy. 
     Provided herein is a method of treating a disease in a subject in need thereof, comprising: administering a pharmaceutical composition to the subject wherein the pharmaceutical composition comprises a polycistronic mRNA sequence encoding a gene or fragment thereof, operably linked to a sequence encoding an L1 retrotransposon; wherein the gene or the fragment thereof is at least 10.1 kb in length. 
     Provided herein is a method for integrating a nucleic acid sequence into the genome of a cell, comprising contacting the cell with a composition comprising a polycistronic mRNA sequence encoding a gene or fragment thereof, operably linked to a sequence encoding an L1 retrotransposon; wherein the gene or the fragment thereof is at least 10.1 kb in length. In some embodiments, the gene or the fragment thereof (e.g., the payload) is at least about 10.2 kb, 10.3 kb, 10.4 kb, 10.5 kb, 10.6 kb, 10.7 kb, 10.8 kb, 10.9 kb, 11 kb, 12 kb, 13 kb, 14 kb, 15 kb, 16 kb, 17 kb, 18 kb, 19 kb, 20 kb or more in length. 
     Provided herein is a method for integrating a nucleic acid sequence into the genome of a cell, comprising contacting the cell with a composition comprising a polycistronic mRNA sequence encoding a gene or fragment thereof, operably linked to a sequence encoding an L1 retrotransposon; wherein the gene or the fragment thereof is selected from a group consisting of ABCA4, MY07A, CEP290, CDH23, EYS, USH2a, GPR98, ALMS1, GDE, OTOF, and F8. 
     Provided herein is a method of expressing a protein encoded by a recombinant nucleic acid in a cell, the method comprising integrating a nucleic acid sequence into the genome of a cell by contacting the cell with a composition comprising a polycistronic mRNA sequence encoding a gene or fragment thereof, operably linked to a sequence encoding an L1 retrotransposon; and expressing a protein encoded by the gene or fragment thereof, wherein expression of the protein is detectable more than 30 days after (a). 
     In one embodiment of a method described herein, the disease is a genetic disease. 
     Provided herein is a method of treating Stargardt disease, LCA10, USH1D, DFNB12, retinitis pigmentosa (RP) USH2A, USH2C, Alstrom syndrome, Glycogen storage disease III, Non-syndromic deafness, Hemophilia A, or Leber congenital amaurosis in a subject, the method comprising: (i) introducing into the subject an mRNA encoding a suitable gene or a fragment thereof, operably linked to a human L1 transposon, or (ii) introducing to the subject a population of cells comprising an mRNA encoding a suitable gene or a fragment thereof, operably linked to a human L1 transposon. 
     In one embodiment of a method described herein, the method comprises treating Stargardt disease in a subject in need thereof, and wherein the mRNA encodes an ABCA4 gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating Usher Syndrome Type 1b (Usher 1b) disease in a subject in need thereof, and wherein the mRNA encodes an MY07A gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating Leber congenital amaurosis (LCA)10 disease in a subject in need thereof, and wherein the mRNA encodes a CEP290 gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating a User Syndrome Type 1D (USH1D) non-syndromic deafness or hearing loss USH1D, DFN12 disease in a subject in need thereof, and wherein the mRNA encodes a CDH23 gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating a retinitis pigmentosa (RP) disease in a subject in need thereof, and wherein the mRNA encodes an EYS gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating a User Syndrome Type 2A (USH2A) and wherein the mRNA encodes an USH2a gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating a User Syndrome Type 2C (USH2C) and wherein the mRNA encodes a GPR98 gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating an Alstrom Syndrome, and wherein the mRNA encodes an ALMS1 gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating a Glycogen Storage Disease III, and wherein the mRNA encodes a GDE gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating a non-syndromic deafness or hearing loss and wherein the mRNA encodes an OTOF gene, or a fragment thereof. 
     In one embodiment of a method described herein, the method comprises treating Hemophilia A, and the mRNA encodes an Factor VIII (F8) gene, or a fragment thereof. 
     Provided herein is a method for targeted replacement of a genomic nucleic acid sequence of a cell, the method comprising: (A) introducing to the cell a polynucleotide sequence encoding a first protein complex comprising a targeted excision machinery for excising from the genome of the cell a nucleic acid sequence comprising one or more mutations; and (B) a recombinant mRNA encoding a second protein complex, wherein the recombinant mRNA comprises: (i) a nucleic acid sequence comprising the excised nucleic acid sequence in (A) that does not contain the one or more mutations, and (ii) a sequence encoding an L1 retrotransposon ORF2 protein under the influence of an independent promoter. 
     In one embodiment of a method described herein, the nucleic acid sequence comprising the one or more mutations comprises a pathogenic variant of a cellular gene. 
     In one embodiment of a method described herein, the a nucleic acid sequence in (B) comprising the nucleic acid sequence that does not contain the one or more mutations is operably linked to the ORF2 sequence. 
     In one embodiment of a method described herein, the method further comprising introducing a sequence comprising a plurality of thymidine residues at the excision site. 
     In some embodiment, introducing the sequence comprises introducing at least four thymidine residues. 
     In one embodiment of a method described herein, the targeted excision machinery comprises a sequence guided site-specific excision endonuclease. 
     In one embodiment of a method described herein, the targeted excision machinery comprises a CRISPR-CAS system. 
     In some embodiments, the targeted excision machinery is a modified recombinant LINE 1 (L1) endonuclease. 
     In some embodiments, introducing the sequence comprising a plurality of thymidine residues comprises base extension by prime editing at the excision site. 
     In some embodiments, the mRNA sequence encoding an L1 retrotransposon ORF2 protein further comprises a sequence encoding the L1 retrotransposon ORF1 protein. 
     In some embodiments, the mRNA comprises a sequence for an inducible promoter. 
     In one embodiment of a method described herein, the excised sequence is greater than 1000 bases. 
     In one embodiment of a method described herein, the excised sequence is greater than 6 kb. 
     In one embodiment of a method described herein, the excised sequence is about 10 kb. 
     In some embodiments, the cell is a lymphocyte. In some embodiments, the cell is a myeloid cell. 
     In some embodiments, the cell is an epithelial cell. In some embodiments, the cell is a cancer cell. 
     In some embodiments, the nucleic acid sequence encodes an ATP-binding cassette (ABC) transporter gene, (ABCA4) gene, or a fragment thereof. 
     In some embodiments, the nucleic acid sequence encodes an MY07A, CEP290, CDH23, EYS, USH2a, GPR98, ALMS1, GDE, OTOF or an F8 gene or a fragment thereof. 
     In some embodiments, introducing comprises introducing to the cell ex vivo. In some embodiments, introducing comprises electroporation. In some embodiments, introducing comprises introducing to the cell in vivo. In some embodiments, expression of the nucleic acid sequence comprising the sequence that does not contain the one or more mutations, is detectable at least 35 days after introducing to the cell. In some embodiments, introducing into the subject comprises direct administration of the mRNA systemically. 
     In some embodiments, introducing into the subject comprises local administration of the mRNA. 
     In some embodiments, the mRNA sequence comprises a cell targeting moiety. 
     In some embodiments, the cell targeting moiety is an aptamer. 
     In some embodiments, introducing into the subject comprises introducing the mRNA in the retina of the subject. 
     Provided herein is a method of integrating a nucleic acid sequence into a genome of a cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA into the cell, wherein the mRNA comprises: (a) an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence, or (ii) a sequence that is a reverse complement of the exogenous sequence; (b) a 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises a binding site for a human ORF protein, and wherein the insert sequence is integrated into the genome of the cell, wherein the insert sequence is a gene selected from a group consisting of ABCA4, MY07A, CEP290, CDH23, EYS, USH2a, GPR98, ALMS1, GDE, OTOF, and F8. 
     In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a binding site for human ORF2p. 
     Provided herein is a method for integrating a nucleic acid sequence into the genome of an immune cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA, wherein the mRNA comprises: (a) an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence or (ii) a sequence that is a reverse complement of the exogenous sequence; (b) 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence, wherein the 5′ UTR sequence or the 3′ UTR sequence comprises an endonuclease binding site and/or a reverse transcriptase binding site, and wherein the insert sequence is integrated into the genome of the immune cell, wherein the insert sequence is a gene selected from a group consisting of ABCA4, MY07A, CEP290, CDH23, EYS, USH2a, GPR98, ALMS1, GDE, OTOF, and F8. 
     Provided herein is a method for integrating a nucleic acid sequence into the genome of a cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA, wherein the mRNA comprises: (a) an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence or (ii) a sequence that is a reverse complement of the exogenous sequence; (b) a 5′ UTR sequence, a sequence of a human retrotransposon downstream of the 5′ UTR sequence, and a 3′ UTR sequence downstream of the sequence of a human retrotransposon; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises an endonuclease binding site and/or a reverse transcriptase binding site, and wherein the sequence of a human retrotransposon encodes for two proteins that are translated from a single RNA containing two ORFs, and wherein the insert sequence is integrated into the genome of the cell, wherein the insert sequence is a gene selected from a group consisting of ABCA4, MY07A, CEP290, CDH23, EYS, USH2a, GPR98, ALMS1, GDE, OTOF, and F8. 
     In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises an ORF2p binding site. In some embodiments, the ORF2p binding site is a poly A sequence in the 3′ UTR sequence. 
     In some embodiments, the mRNA comprises a sequence of a human retrotransposon. In some embodiments, the sequence of a human retrotransposon is downstream of the 5′ UTR sequence. 
     In some embodiments, the sequence of a human retrotransposon is upstream of the 3′ UTR sequence. In some embodiments, the sequence of a human retrotransposon encodes for two proteins that are translated from a single RNA containing two ORFs. In some embodiments, the two ORFs are non-overlapping ORFs. 
     In some embodiments, the sequence of a human retrotransposon comprises a sequence of a non-LTR retrotransposon. In some embodiments, the sequence of a human retrotransposon encodes comprises a LINE-1 retrotransposon. In some embodiments, the LINE-1 retrotransposon is a human LINE-1 retrotransposon. In some embodiments, the sequence of a human retrotransposon comprises a sequence encoding an endonuclease and/or a reverse transcriptase. 
     In some embodiments, the endonuclease and/or a reverse transcriptase is ORF2p. 
     In some embodiments, the reverse transcriptase is a group II intron reverse transcriptase domain. 
     In some embodiments, the endonuclease and/or a reverse transcriptase is a minke whale endonuclease and/or a reverse transcriptase. 
     In some embodiments, the sequence of a human retrotransposon comprises a sequence encoding ORF2p. In some embodiments, the insert sequence is integrated into the genome at a poly T site using specificity of an endonuclease domain of the ORF2p. In some embodiments, the poly T site comprises the sequence TTTTTA. In some embodiments, the retrotransposon comprises an ORF1p and/or the ORF2p fused to a nuclear retention sequence. In some embodiments, the nuclear retention sequence is an Alu sequence. In some embodiments, the ORF1p and/or the ORF2p is fused to an MS2 coat protein. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises at least one, two, three or more MS2 hairpin sequences. 
     Provided herein is a composition comprising a recombinant mRNA or vector encoding an mRNA, wherein the mRNA comprises a human LINE-1 transposon sequence comprising: (i) a human LINE-1 transposon 5′ UTR sequence, (ii) a sequence encoding ORF1p downstream of the human LINE-1 transposon 5′ UTR sequence, (iii) an inter-ORF linker sequence downstream of the sequence encoding ORF1p, (iv) a sequence encoding ORF2p downstream of the inter-ORF linker sequence, and (v) a 3′ UTR sequence derived from a human LINE-1 transposon downstream of the sequence encoding ORF2p; wherein the 3′ UTR sequence comprises an insert sequence, wherein the insert sequence is a reverse complement of a sequence encoding an exogenous polypeptide or a reverse complement of a sequence encoding an exogenous regulatory element, wherein the insert sequence is a gene selected from a group consisting of ABCA4, MY07A, CEP290, CDH23, EYS, USH2a, GPR98, ALMS1, GDE, OTOF, and F8. 
     Provided herein is a composition comprising a nucleic acid comprising a nucleotide sequence encoding (a) a long interspersed nuclear element (LINE) polypeptide, wherein the LINE polypeptide includes human ORF1p and human ORF2p; and (b) an insert sequence, wherein the insert sequence is a reverse complement of a sequence encoding an exogenous polypeptide or a reverse complement of a sequence encoding an exogenous regulatory element, wherein the composition is substantially non-immunogenic, wherein the insert sequence is a gene selected from a group consisting of ABCA4, MY07A, CEP290, CDH23, EYS, USH2a, GPR98, ALMS1, GDE, OTOF, and F8. 
     Immunotherapy using phagocytic cells involves making and using engineered myeloid cells, such as macrophages or other phagocytic cells that attack and kill diseased cells, such as cancer cells, or infected cells. Engineered myeloid cells, such as macrophages and other phagocytic cells are prepared by incorporating in them via recombinant nucleic acid technology, a synthetic, recombinant nucleic acid encoding an engineered protein, such as a chimeric antigen receptor, that comprises a targeted antigen binding extracellular domain that is designed to bind to specific antigens on the surface of a target, such as a target cell, such as a cancer cell. Binding of the engineered chimeric receptor to an antigen on a target, such as cancer antigen (or likewise, a disease target), initiates phagocytosis of the target. This triggers two fold action: one, phagocytic engulfment and lysis of the target destroys the target and eliminates it as a first line of immune defense; two, antigens from the target are digested in the phagolysosome of the myeloid cell, are presented on the surface of the myeloid cell, which then leads to activation of T cells and further activation of the immune response and development of immunological memory. Chimeric receptors are engineered for enhanced phagocytosis and immune activation of the myeloid cell in which it is incorporated and expressed. Chimeric antigen receptors of the disclosure are variously termed herein as a chimeric fusion protein, CFP, phagocytic receptor (PR) fusion protein (PFP), or chimeric antigen receptor for phagocytosis (CAR-P), while each term is directed to the concept of a recombinant chimeric and/or fusion receptor protein. In some embodiments, genes encoding non-receptor proteins are also co-expressed in the myeloid cells, typically for an augmentation of the chimeric antigen receptor function. In summary, contemplated herein are various engineered receptor and non-receptor recombinant proteins that are designed to augment phagocytosis and or immune response of a myeloid cell against a disease target, and methods and compositions for creating and incorporating recombinant nucleic acids that encode the engineered receptors or non-receptor recombinant protein, such that the methods and compositions are suitable for creating an engineered myeloid cell for immunotherapy. 
     In one aspect, the present disclosure provides compositions and methods for stable gene transfer into a cell, where the cell can be any somatic cell. In some embodiments the compositions and methods are designed for cell-specific or tissue-specific delivery. In some cases, the methods described herein relate to supplying a functional protein or a fragment thereof to compensate for an absent or defective (mutated) protein in vivo, e.g., for a protein replacement therapy. 
     Incorporation of a recombinant nucleic acid in a cell can be accomplished by one or more gene transfer techniques that are available in the state of the art. However, incorporation of exogenous genetic (e.g., nucleic acid) elements into the genome for therapeutic purposes still faces several challenges. Achieving stable integration in a safe and dependable manner, and efficient and prolonged expression are a few among them. Most of the successful gene transfer systems aimed at genomic integration of the cargo nucleic acid sequence rely on viral delivery mechanisms, which have some inherent safety and efficacy issues. Delivery and integration of long nucleic acid sequences cannot be achieved by current gene editing systems. 
     Little attention has so far been devoted to making and using engineered myeloid cells for stable long-term gene transfer and expression of the transgene. For example, gene transfer to differentiated mammalian cells ex vivo for cell therapy can be accomplished via viral gene transfer mechanisms. However, there are several strategic disadvantages associated with the use of viral gene-transfer vectors, including an undesired potential for transgene silencing over time, the preferential integration into transcriptionally active sites of the genome with associated undesired activation of other genes (e.g. oncogenes) and genotoxicity. In addition to the safety issues increased expense and cumbersome effort of manufacturing, storing and handling integrating viruses often stand in the way of large-scale use of viral vector mediated of gene-modified cells in therapeutic applications. These persistent concerns associated with viral vectors regarding safety, as well as cost and scale of vector production necessitates alternative methods for effective therapy. 
     Integration of a transgene into the genome of a cell to be used for an immunotherapy can be advantageous in the sense that it is stable and a lower number of cells is required for delivery during the therapy. On the other hand, integrating a transgene in a non-dividing cell can be challenging in both affecting the health and function of the cell as well as the ultimate lifespan of the cell in vivo, and therefore affects its overall utility as the therapeutic. In some embodiments, the methods described herein for generating a myeloid cell for immunotherapy can be a cumulative product of a number of steps and compositions involving but not limited to, for example, selecting a myeloid cell for modifying; method and compositions for incorporating a recombinant nucleic acid in a myeloid cell; methods and compositions for enhancing expression of the recombinant nucleic acid; methods and compositions for selecting and modifying vectors; methods of preparing a recombinant nucleic acid suitable for in vivo administration for uptake and incorporation of the recombinant nucleic acid by a myeloid cell in vivo and therefore generating a myeloid cell for therapy. In some aspects, one or more embodiments of the various inventions described herein are transferrable among each other, and one of skill in the art is expected to use them in alternatives, combinations or interchangeably without the necessity of undue experimentation. All such variations of the disclosed elements are contemplated and fully encompassed herein. 
     In one aspect, transposons, or transposable elements (TEs) are considered herein, for means of incorporating a heterologous, synthetic or recombinant nucleic acid encoding a transgene of interest in a myeloid cell. Transposon, or transposable elements are genetic elements that have the capability to transpose fragments of genetic material into the genome by use of an enzyme known as transposase. Mammalian genomes contain a high number of transposable element (TE)-derived sequences, and up to 70% of our genome represents TE-derived sequences (de Koning et al. 2011; Richardson et al. 2015). These elements could be exploited to introduce genetic material into the genome of a cell. The TE elements are capable of mobilization, often termed as “jumping” genetic material within the genome. TEs generally exist in eukaryotic genomes in a reversibly inactive, epigenetically silenced form. In the present disclosure methods and compositions for efficient and stable integration of transgenes into macrophages and other phagocytic cells. The method is based on use of a transposase and transposable elements mRNA-encoded transposase. In some embodiments, Long Interspersed Element-1 (L1) RNAs are used for stable integration and/or retrotransposition of the transgene into a cell (e.g., a macrophage or phagocytic cell. 
     Contemplated herein are methods for retrotransposon mediated stable integration of an exogenous nucleic acid sequence into the genome of a cell. The method may take advantage of the random genomic integration machinery of the retrotransposon into the cell without creating an adverse effect. Methods described herein can be used for robust and versatile incorporation of an exogenous nucleic acid sequence into a cell, such that the exogenous nucleic acid is incorporated at a safe locus within the genome and is expressed without being silenced by the cell&#39;s inherent defense mechanism. The method described herein can be used to incorporate an exogenous nucleic acid that is about 1 kb, about 2 kb, about 3 kb, about 4 kb, about 5 kb, about 6 kb, about 7 kb about 8 kb, about 9 kb, about 10 kb, or more in size. In some embodiments, the exogenous nucleic acid is not incorporated within a ribosomal locus. In some embodiments, the exogenous nucleic acid is not incorporated within a ROSA26 locus, or another safe harbor locus. In some embodiments, the methods and compositions described herein can incorporate an exogenous nucleic acid sequence anywhere within the genome of the cell. Furthermore, contemplated herein is a retrotransposition system that is developed to incorporate an exogenous nucleic acid sequence into a specific predetermined site within the genome of a cell, without creating an adverse effect. The disclosed methods and compositions incorporate several mechanisms of engineering the retrotransposons for highly specific incorporation of the exogenous nucleic acid into a cell with high fidelity. Retrotransposons chosen for this purpose may be a human retrotransposon. 
     Methods and compositions described herein represent a salient breakthrough in the molecular systems and mechanisms for manipulating the genome of a cell. Shown here for the first time is a method that exploits a human retrotransposon system into non-virally delivering and stably integrating a large fragment of exogenous nucleic acid sequence (at least greater than 100 nucleobases, at least greater than 1 kb, at least greater than 2 kb, at least greater than 3 kb, etc.) into a non-conserved region of the genome that is not an rDNA or a ribosomal locus or a designated safe-harbor locus such as the ROSA 26 locus. 
     In some embodiments, a retrotransposable system is used to stably incorporate into the genome and express a non-endogenous nucleic acid, where the non-endogenous nucleic acid comprises retrotransposable elements within the nucleic acid sequence. In some embodiments, a cell&#39;s endogenous retrotransposable system (e.g., proteins and enzymes) is used to stably express a non-endogenous nucleic acid in the cell. In some embodiments, a cell&#39;s endogenous retrotransposable system (e.g., proteins and enzymes, such as a LINE-1 retrotransposition system) is used, but may further express one or more components of the retrotransposable system to stably express a non-endogenous nucleic acid in the cell. 
     In some embodiments, a synthetic nucleic acid is provided herein, the synthetic nucleic acid encoding a transgene, and encoding one or more components for genomic integration and/or retrotransposition. 
     In one aspect, provided herein is a method of integrating a nucleic acid sequence into a genome of a cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA into the cell, wherein the mRNA comprises: an insert sequence, wherein the insert sequence comprises an exogenous sequence, or a sequence that is a reverse complement of the exogenous sequence; a 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises a binding site for a human ORF protein, and wherein the insert sequence is integrated into the genome of the cell. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a binding site for human ORF2p. 
     In one aspect, provided herein is a method for integrating a nucleic acid sequence into the genome of an immune cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA, wherein the mRNA comprises an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence or (ii) a sequence that is a reverse complement of the exogenous sequence; 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence, wherein the 5′ UTR sequence or the 3′ UTR sequence comprises an endonuclease binding site and/or a reverse transcriptase binding site, and wherein the transgene sequence is integrated into the genome of the immune cell. 
     In one aspect, provided herein is a method for integrating a nucleic acid sequence into the genome of a cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA, wherein the mRNA comprises an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence or (ii) a sequence that is a reverse complement of the exogenous sequence; a 5′ UTR sequence, a sequence of a human retrotransposon downstream of the 5′ UTR sequence, and a 3′ UTR sequence downstream of the sequence of a human retrotransposon; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises an endonuclease binding site and/or a reverse transcriptase binding site, and wherein the sequence of a human retrotransposon encodes for two proteins that are translated from a single RNA containing two ORFs, and wherein the insert sequence is integrated into the genome of the cell. 
     In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises an ORF2p binding site. In some embodiments, the ORF2p binding site is a poly A sequence in the 3′ UTR sequence. 
     In some embodiments, the mRNA comprises a sequence of a human retrotransposon. In some embodiments, the sequence of a human retrotransposon is downstream of the 5′ UTR sequence. In some embodiments, the sequence of a human retrotransposon is upstream of the 3′ UTR sequence. In some embodiments, the polynucleotide sequence that is desired to be transferred and incorporated into the genome of a cell (e.g., the insert) is inserted at a site 3′ to the sequence encoding ORF1 in a recombinant nucleic acid construct. In some embodiments, the polynucleotide sequence that is desired to be transferred and incorporated into the genome of a cell is inserted at a site 3′ to the sequence encoding ORF2 in a recombinant nucleic acid construct. In some embodiments the sequence that is desired to be transferred and incorporated into the genome of a cell is inserted within the 3′-UTR of ORF1 or ORF2, or both. In some embodiments, the polynucleotide sequence that is sequence that is desired to be transferred and incorporated into the genome of a cell is inserted upstream of the poly A tail of ORF2 in a recombinant nucleic acid construct. 
     In some embodiments, the sequence of a human retrotransposon encodes for two proteins that are translated from a single RNA containing two ORFs. In some embodiments, the two ORFs are non-overlapping ORFs. In some embodiments, the two ORFs are ORF1 and ORF2. In some embodiments, the ORF1 encodes ORF1p and ORF2 encodes ORF2p. 
     In some embodiments, the sequence of a human retrotransposon comprises a sequence of a non-LTR retrotransposon. In some embodiments, the sequence of a human retrotransposon comprises a LINE-1 retrotransposon. In some embodiments, the LINE-1 retrotransposon is a human LINE-1 retrotransposon. In some embodiments, the sequence of a human retrotransposon comprises a sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the endonuclease and/or a reverse transcriptase is ORF2p. In some embodiments, the reverse transcriptase is a group II intron reverse transcriptase domain. In some embodiments, the endonuclease and/or a reverse transcriptase is a minke whale endonuclease and/or a reverse transcriptase. In some embodiments, the sequence of a human retrotransposon comprises a sequence encoding ORF2p. In some embodiments, the insert sequence is integrated into the genome at a poly T site using specificity of an endonuclease domain of the ORF2p. In some embodiments, the poly T site comprises the sequence TTTTTA. 
     In some embodiments, provided herein is a polynucleotide construct comprising an mRNA wherein the mRNA comprises a sequence encoding a human retrotransposon, wherein, (i) the sequence of a human retrotransposon comprises a sequence encoding ORF1p, (ii) the mRNA does not comprise a sequence encoding ORF1p, or (iii) the mRNA comprises a replacement of the sequence encoding ORF1p with a 5′ UTR sequence from the complement gene. In some embodiments, the mRNA comprises a first mRNA molecule encoding ORF1p, and a second mRNA molecule encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the mRNA is an mRNA molecule comprising a first sequence encoding ORF1p, and a second sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the first sequence encoding ORF1p and the second sequence encoding an endonuclease and/or a reverse transcriptase are separated by a linker sequence. 
     In some embodiments, the linker sequence comprises an internal ribosome entry sequence (IRES). In some embodiments, the IRES is an IRES from CVB3 or EV71. In some embodiments, the linker sequence encodes a self-cleaving peptide sequence. In some embodiments, the linker sequence encodes a T2A, a E2A or a P2A sequence 
     In some embodiments, the sequence of a human retrotransposon comprises a sequence that encodes ORF1p fused to an additional protein sequence and/or a sequence that encodes ORF2p fused to an additional protein sequence. In some embodiments, the ORF1p and/or the ORF2p is fused to a nuclear retention sequence. In some embodiments, the nuclear retention sequence is an Alu sequence. In some embodiments, the ORF1p and/or the ORF2p is fused to an MS2 coat protein. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises at least one, two, three or more MS2 hairpin sequences. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a sequence that promotes or enhances interaction of a poly A tail of the mRNA with the endonuclease and/or a reverse transcriptase. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a sequence that promotes or enhances interaction of a poly-A-binding proteins (e.g., PABP) with the endonuclease and/or a reverse transcriptase. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a sequence that increases specificity of the endonuclease and/or a reverse transcriptase to the mRNA relative to another mRNA expressed by the cell. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises an Alu element sequence. 
     In some embodiments, the first sequence encoding ORF1p and the second sequence encoding an endonuclease and/or a reverse transcriptase have the same promoter. In some embodiments, the insert sequence has a promoter that is different from the promoter of the first sequence encoding ORF1p. In some embodiments, the insert sequence has a promoter that is different from the promoter of the second sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the first sequence encoding ORF1p and/or the second sequence encoding an endonuclease and/or a reverse transcriptase have a promoter or transcription initiation site selected from the group consisting of an inducible promoter, a CMV promoter or transcription initiation site, a T7 promoter or transcription initiation site, an EF1a promoter or transcription initiation site and combinations thereof. In some embodiments, the insert sequence has a promoter or transcription initiation site selected from the group consisting of an inducible promoter, a CMV promoter or transcription initiation site, a T7 promoter or transcription initiation site, an EF1a promoter or transcription initiation site and combinations thereof. 
     In some embodiments, the first sequence encoding ORF1p and the second sequence encoding an endonuclease and/or a reverse transcriptase are codon optimized for expression in a human cell. 
     In some embodiments, the mRNA comprises a WPRE element. In some embodiments, the mRNA comprises a selection marker. In some embodiments, the mRNA comprises a sequence encoding an affinity tag. In some embodiments, the affinity tag is linked to the sequence encoding an endonuclease and/or a reverse transcriptase. 
     In some embodiments, the 3′ UTR comprises a poly A sequence or wherein a poly A sequence is added to the mRNA in vitro. In some embodiments, the poly A sequence is downstream of a sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the insert sequence is upstream of the poly A sequence. 
     In some embodiments, the 3′ UTR sequence comprises the insert sequence. In some embodiments, the insert sequence comprises a sequence that is a reverse complement of the sequence encoding the exogenous polypeptide. In some embodiments, the insert sequence comprises a polyadenylation site. In some embodiments, the insert sequence comprises an SV40 polyadenylation site. In some embodiments, the insert sequence comprises a polyadenylation site upstream of the sequence that is a reverse complement of the sequence encoding the exogenous polypeptide. In some embodiments, the insert sequence is integrated into the genome at a locus that is not a ribosomal locus. In some embodiments, the insert sequence is integrated into the genome at a locus that is not a rDNA locus. In some embodiments, the insert sequence integrates into a gene or regulatory region of a gene, thereby disrupting the gene or downregulating expression of the gene. In some embodiments, the insert sequence integrates into a gene or regulatory region of a gene, thereby upregulating expression of the gene. In some embodiments, the insert sequence integrates into the genome and replaces a gene. In some embodiments, the insert sequence is stably integrated into the genome. In some embodiments, the insert sequence is retrotransposed into the genome. In some embodiments, the insert sequence is integrated into the genome by cleavage of a DNA strand of a target site by an endonuclease encoded by the mRNA. In some embodiments, the insert sequence is integrated into the genome via target-primed reverse transcription (TPRT). In some embodiments, the insert sequence is integrated into the genome via reverse splicing of the mRNA into a DNA target site of the genome. 
     In some embodiments, the cell is an immune cell. In some embodiments, the immune cell is a T cell or a B cell. In some embodiments, the immune cell is a myeloid cell. In some embodiments, the immune cell is selected from a group consisting of a monocyte, a macrophage, a dendritic cell, a dendritic precursor cell, and a macrophage precursor cell. 
     In some embodiments, the mRNA is a self-integrating mRNA. In some embodiments, the method comprises introducing into the cell the mRNA. In some embodiments, the method comprises introducing into the cell the vector encoding the mRNA. In some embodiments, the method comprises introducing the mRNA or the vector encoding the mRNA into a cell ex vivo. In some embodiments, the method further comprises administering the cell to a human subject. In some embodiments, the method comprises administering the mRNA or the vector encoding the mRNA to a human subject. In some embodiments, an immune response is not elicited in the human subject. In some embodiments, the mRNA or the vector is substantially non-immunogenic. 
     In some embodiments, the vector is a plasmid or a viral vector. In some embodiments, the vector comprises a non-LTR retrotransposon. In some embodiments, the vector comprises a human L1 element. In some embodiments, the vector comprises a L1 retrotransposon ORF1 gene. In some embodiments, the vector comprises a L1 retrotransposon ORF2 gene. In some embodiments, the vector comprises a L1 retrotransposon. In some embodiments, provided herein is an mRNA comprising sequences encoding human LINE 1 retrotransposition elements, and a payload comprising a nucleic acid sequence which can be retrotransposed and integrated into a genome of a cell comprising the mRNA. In some embodiments, provided herein is an mRNA that can be delivered into a living cell, e.g., a human cell, wherein, the mRNA comprises sequences encoding human LINE 1 retrotransposition elements, and a payload comprising a nucleic acid sequence which can be retrotransposed and integrated into the genome of the cell. In some embodiments, the sequences encoding human LINE 1 retrotransposition elements comprise a L1 retrotransposon ORF1 sequence or a fragment thereof. In some embodiments, the sequences encoding human LINE 1 retrotransposition elements comprise a L1 retrotransposon ORF2 sequence or a fragment thereof. In some embodiments, the sequences encoding human LINE 1 retrotransposition elements comprise a L1 retrotransposon ORF1 sequence or a fragment thereof and a L1 retrotransposon ORF2 sequence or a fragment thereof, and a nucleic acid “payload” sequence which is a heterologous sequence which is integrated into the genome of cell by retrotransposition. (See, for example,  FIG.  1 B ). 
     In some embodiments, the mRNA is at least about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, or 3 kilobases. In some embodiments, the mRNA is a most about 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9 or 5 kilobases. In some embodiments, the mRNA is at least about 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9 or 6 kilobases. In some embodiments, the mRNA is at least about 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9 or 7 kilobases. In some embodiments, the mRNA is at least about 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9 or 8 kilobases. In some embodiments, the mRNA is at least about 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9 or 9 kilobases. In some embodiments, the mRNA is at least about 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9 or 10 kilobases. 
     In some embodiments, the mRNA comprises a sequence that inhibits or prevents degradation of the mRNA. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA inhibits or prevents degradation of the mRNA by an exonuclease or an RNAse. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA is a G quadruplex, pseudoknot or triplex sequence. In some embodiments, the sequence the sequence that inhibits or prevents degradation of the mRNA is an exoribonuclease-resistant RNA structure from a flavivirus RNA or an ENE element from KSV. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA inhibits or prevents degradation of the mRNA by a deadenylase. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA comprises non-adenosine nucleotides within or at a terminus of a poly A tail of the mRNA. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA increases stability of the mRNA. In some embodiments, the exogenous sequence comprises a sequence encoding an exogenous polypeptide. In some embodiments, the sequence encoding an exogenous polypeptide is not in frame with a sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the sequence encoding an exogenous polypeptide is not in frame with a sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the exogenous sequence does not comprise introns. In some embodiments, the exogenous sequence comprises a sequence encoding an exogenous polypeptide selected from the group consisting of an enzyme, a receptor, a transport protein, a structural protein, a hormone, an antibody, a contractile protein and a storage protein. In some embodiments, the exogenous sequence comprises a sequence encoding an exogenous polypeptide selected from the group consisting of a chimeric antigen receptor (CAR), a ligand, an antibody, a receptor, and an enzyme. In some embodiments, the exogenous sequence comprises a regulatory sequence. In some embodiments, the regulatory sequence comprises a cis-acting regulatory sequence. In some embodiments, the regulatory sequence comprises a cis-acting regulatory sequence selected from the group consisting of an enhancer, a silencer, a promoter or a response element. In some embodiments, the regulatory sequence comprises a trans-acting regulatory sequence. In some embodiments, the regulatory sequence comprises a trans-acting regulatory sequence that encodes a transcription factor. 
     In some embodiments, integration of the insert sequence does not adversely affect cell health. In some embodiments, the endonuclease, the reverse transcriptase or both are capable of site-specific integration of the insert sequence. 
     In some embodiments, the retrotransposon system used herein is further engineered for precise site-specific integration. In some embodiments the retrotransposon system used herein is paired with a CRISPR-Cas system to increase specificity. In some embodiments, the ORF polypeptide-binding sequence, e.g., TTTTTA may be engineered site-specifically into a genomic sequence of a cell. 
     In some embodiments, the mRNA comprises a sequence encoding an additional nuclease domain or a nuclease domain that is not derived from ORF2. In some embodiments, the mRNA comprises a sequence encoding a megaTAL nuclease domain, a TALEN domain, a Cas9 domain, a zinc finger binding domain from an R2 retroelement, or a DNA binding domain that binds to repetitive sequences such as a Rep78 from AAV. In some embodiments, the endonuclease comprises a mutation that reduces activity of the endonuclease compared to the endonuclease without the mutation. In some embodiments, the endonuclease is an ORF2p endonuclease and the mutation is S228P. In some embodiments, the mRNA comprises a sequence encoding a domain that increases fidelity and/or processivity of the reverse transcriptase. In some embodiments, the reverse transcriptase is a reverse transcriptase from a retroelement other than ORF2 or reverse transcriptase that has higher fidelity and/or processivity compared to a reverse transcriptase of ORF2p. In some embodiments, the reverse transcriptase is a group II intron reverse transcriptase. In some embodiments, the group II intron reverse transcriptase is a group IIA intron reverse transcriptase, a group IIB intron reverse transcriptase, or a group IIC intron reverse transcriptase. In some embodiments, the group II intron reverse transcriptase is TGIRT-II or TGIRT-III. 
     In some embodiments, the mRNA comprises a sequence comprising an Alu element and/or a ribosome binding aptamer. In some embodiments, the mRNA comprises a sequence encoding a polypeptide comprising a DNA binding domain. In some embodiments, the 3′ UTR sequence is derived from a viral 3′ UTR or a beta-globin 3′ UTR. 
     In one aspect, provided herein is a composition comprising a recombinant mRNA or vector encoding an mRNA, wherein the mRNA comprises a human LINE-1 transposon sequence comprising a human LINE-1 transposon 5′ UTR sequence, a sequence encoding ORF1p downstream of the human LINE-1 transposon 5′ UTR sequence, an inter-ORF linker sequence downstream of the sequence encoding ORF1p, a sequence encoding ORF2p downstream of the inter-ORF linker sequence, and a 3′ UTR sequence derived from a human LINE-1 transposon downstream of the sequence encoding ORF2p; wherein the 3′ UTR sequence comprises an insert sequence, wherein the insert sequence is a reverse complement of a sequence encoding an exogenous polypeptide or a reverse complement of a sequence encoding an exogenous regulatory element. 
     In some embodiments, the insert sequence integrates into the genome of a cell when introduced into the cell. In some embodiments, the insert sequence integrates into a gene associated a condition or disease, thereby disrupting the gene or downregulating expression of the gene. In some embodiments, the insert sequence integrates into a gene, thereby upregulating expression of the gene. In some embodiments, the recombinant mRNA or vector encoding the mRNA is isolated or purified. 
     In one aspect, provided herein is a composition comprising a nucleic acid comprising a nucleotide sequence encoding (a) a long interspersed nuclear element (LINE) polypeptide, wherein the LINE polypeptide includes human ORF1p and human ORF2p; and (b) an insert sequence, wherein the insert sequence is a reverse complement of a sequence encoding an exogenous polypeptide or a reverse complement of a sequence encoding an exogenous regulatory element, wherein the composition is substantially non-immunogenic. In some embodiments, integration of the insert sequence does not adversely affect cell health. 
     In some embodiments, the composition comprises human ORF1p and human ORF2p proteins. In some embodiments, the composition comprises a ribonucleoprotein (RNP) comprising human ORF1p and human ORF2p complexed to the nucleic acid. In some embodiments, the nucleic acid is mRNA. 
     In one aspect, provided herein is a composition comprising a cell comprising a composition described herein. In some embodiments, the cell is an immune cell. In some embodiments, the immune cell is a T cell or a B cell. In some embodiments, the immune cell is a myeloid cell. In some embodiments, the immune cell is selected from a group consisting of a monocyte, a macrophage, a dendritic cell, a dendritic precursor cell, and a macrophage precursor cell. In some embodiments, the insert sequence is a reverse complement of a sequence encoding an exogenous polypeptide and the exogenous polypeptide is a chimeric antigen receptor (CAR). 
     In one aspect, provided herein is a pharmaceutical composition comprising a composition described herein, and a pharmaceutically acceptable excipient. In some embodiments, the pharmaceutical composition is for use in gene therapy. In some embodiments, the pharmaceutical composition is for use in the manufacture of a medicament for treating a disease or condition. In some embodiments, the pharmaceutical composition is for use in treating a disease or condition. In one aspect, provided herein is a method of treating a disease in a subject, comprising administering a pharmaceutical composition described herein to a subject with a disease or condition. In some embodiments, the method increases an amount or activity of a protein or functional RNA in the subject. In some embodiments, the subject has a deficient amount or activity of a protein or functional RNA. In some embodiments, the deficient amount or activity of a protein or functional RNA is associated with or causes the disease or condition. 
     In some embodiments, the method further comprising administering an agent that inhibits human silencing hub (HUSH) complex, an agent that inhibits FAM208A, or an agent that inhibits TRIM28. In some embodiments, the agent that inhibits human silencing hub (HUSH) complex is an agent that inhibits Periphilin, TASOR and/or MPP8. In some embodiments, the agent that inhibits human silencing hub (HUSH) complex inhibits assembly of the HUSH complex. In some embodiments, the agent inhibits the Fanconi anemia complex. In some embodiments, the agent inhibits FANCD2-FANC1 heterodimer monoubiquitylation. In some embodiments, the agent inhibits FANCD2-FANC1 heterodimer formation. 
     In some embodiments the agent inhibits the Fanconi Anemia (FA) core complex. FA core complex is a component of the Fanconi anemia DNA damage repair pathway, e.g., in chemotherapy induced DNA inter-strand crosslinks. The FA core complex comprises two central diners of the FANCB and FA-associated protein of 100 kDa (FAAP100) subun its, flanked by two copies of the RING finger subunit, FANCL. These two heterotrimers act as a scaffold to assemble the remaining five subunits, resulting in an extended asymmetric structure. Destabilization of the scaffold would disrupt the entire complex, resulting in a non-functional FA pathway. Examples of agents that can inhibit the FA core complex include Bortezomib and curcumin analogs EF24 and 4H-TTD. 
     Accordingly, it is an object of the present invention to provide novel transposon-based vectors useful in providing gene therapy to an animal. It is an object of the present invention to provide novel transposon-based vectors for use in the preparation of a medicament useful in providing gene therapy to an animal or human. It is another object of the present invention to provide novel transposon-based vectors that encode for the production of desired proteins or peptides in cells. Yet another object of the present invention to provide novel transposon-based vectors that encode for the production of desired nucleic acids in cells. It is a further object of the present invention to provide methods for cell and tissue specific incorporation of transposon-based DNA or RNA constructs comprising targeting a selected gene to a specific cell or tissue of an animal. It is yet another object of the present invention to provide methods for cell and tissue specific expression of transposon-based DNA or RNA constructs comprising designing a DNA or RNA construct with cell specific promoters that enhance stable incorporation of the selected gene by the transposase and expressing the selected gene in the cell. It is an object of the present invention to provide gene therapy for generations through germ line administration of a transposon-based vector. Another object of the present invention is to provide gene therapy in animals through non germ line administration of a transposon-based vector. Another object of the present invention is to provide gene therapy in animals through administration of a transposon-based vector, wherein the animals produce desired proteins, peptides or nucleic acids. Yet another object of the present invention is to provide gene therapy in animals through administration of a transposon-based vector, wherein the animals produce desired proteins or peptides that are recognized by receptors on target cells. Still another object of the present invention is to provide gene therapy in animals through administration of a transposon-based vector, wherein the animals produce desired fusion proteins or fusion peptides, a portion of which are recognized by receptors on target cells, in order to deliver the other protein or peptide component of the fusion protein or fusion peptide to the cell to induce a biological response. Yet another object of the present invention is to provide a method for gene therapy of animals through administration of transposon-based vectors comprising tissue specific promoters and a gene of interest to facilitate tissue specific incorporation and expression of a gene of interest to produce a desired protein, peptide or nucleic acid. Another object of the present invention is to provide a method for gene therapy of animals through administration of transposon-based vectors comprising cell specific promoters and a gene of interest to facilitate cell specific incorporation and expression of a gene of interest to produce a desired protein, peptide or nucleic acid. Still another object of the present invention is to provide a method for gene therapy of animals through administration of transposon-based vectors comprising cell specific promoters and a gene of interest to facilitate cell specific incorporation and expression of a gene of interest to produce a desired protein, peptide or nucleic acid, wherein the desired protein, peptide or nucleic acid has a desired biological effect in the animal. 
     In one aspect, provided herein are methods and compositions for delivery inside a cell, for example a myeloid cell and stable incorporation of one or more nucleic acids, comprising nucleic acid sequences encoding one or more proteins, wherein the stable incorporation may be via non-viral mechanisms. In some embodiments, the delivery of a nucleic acid composition into a myeloid cell is via a non-viral mechanism. In some embodiments, the delivery of the nucleic acids may further bypass plasmid mediated delivery. A “plasmid,” as used herein, refers to a non-viral expression vector, e.g., a nucleic acid molecule that encodes for genes and/or regulatory elements necessary for the expression of genes. A “viral vector,” as used herein, refers to a viral-derived nucleic acid that is capable of transporting another nucleic acid into a cell. A viral vector is capable of directing expression of a protein or proteins encoded by one or more genes carried by the vector when it is present in the appropriate environment. Examples for viral vectors include, but are not limited to retroviral, adenoviral, lentiviral and adeno-associated viral vectors. 
     In some embodiments, provided herein is a method of delivering a composition inside a cell, such as in a myeloid cell, the composition comprising one or more nucleic acid sequences encoding one or more proteins, wherein the one or more nucleic acid sequences is an RNA. In some embodiments, the RNA is mRNA. 
     In some embodiments, one or more mRNA comprising one or more nucleic acid sequences are delivered. In some embodiments, the one or more mRNA may comprise at least one modified nucleotide. The term “nucleotide,” as used herein, refers to a base-sugar-phosphate combination. A nucleotide may comprise a synthetic nucleotide. A nucleotide may comprise a synthetic nucleotide analog. Nucleotides may be monomeric units of a nucleic acid sequence (e.g. deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)). The term nucleotide may include ribonucleoside triphosphates adenosine triphosphate (ATP), uridine triphosphate (UTP), cytosine triphosphate (CTP), guanosine triphosphate (GTP) and deoxyribonucleoside triphosphates such as dATP, dCTP, dITP, dUTP, dGTP, or derivatives thereof. Such derivatives may include, for example, [aS]dATP, 7-deaza-dGTP and 7-deaza-dATP, and nucleotide derivatives that confer nuclease resistance on the nucleic acid molecule containing them. The term nucleotide as used herein may refer to dideoxyribonucleoside triphosphates (ddNTPs) and their derivatives. Illustrative examples of dideoxyribonucleoside triphosphates may include, but are not limited to, ddATP, ddCTP, ddGTP, ddITP, and ddTTP. A nucleotide may be unlabeled or detectably labeled by well-known techniques. Labeling may also be carried out with quantum dots. Detectable labels may include, for example, radioactive isotopes, fluorescent labels, chemiluminescent labels, bioluminescent labels and enzyme labels. Fluorescent labels of nucleotides may include but are not limited fluorescein, 5-carboxyfluorescein (FAM), 2′7′-dimethoxy-4′5-dichloro-6-carboxyfluorescein (JOE), rhodamine, 6-carboxyrhodamine (R6G), N,N,NcN′-tetramethyl-6-carboxyrhodamine (TAMRA), 6-carboxy-X-rhodamine (ROX), 4-(4′dimethylaminophenylazo) benzoic acid (DABCYL), Cascade Blue, Oregon Green, Texas Red, Cyanine and 5-(2′-aminoethyl)aminonaphthalene-1-sulfonic acid (EDANS). Specific examples of fluorescently labeled nucleotides may include [R6G]dUTP, [TAMRA]dUTP, [R110]dCTP, [R6G]dCTP, [TAMRA]dCTP, [JOE]ddATP, [R6G]ddATP, [FAM]ddCTP, [R110]ddCTP, [TAN1RA]ddGTP, [ROX]ddTTP, [dR6G]ddATP, [dR110]ddCTP, [dTAMRA]ddGTP, and [dROX]ddTTP available from Perkin Elmer, Foster City, Calif. FluoroLink DeoxyNucleotides, FluoroLink Cy3-dCTP, FluoroLink Cy5-dCTP, FluoroLink Fluor X-dCTP, FluoroLink Cy3-dUTP, and FluoroLink Cy5-dUTP available from Amersham, Arlington Heights, Ill.; Fluorescein-15-dATP, Fluorescein-12-dUTP, Tetramethyl-rodamine-6-dUTP, TR770-9-dATP, Fluorescein-12-ddUTP, Fluorescein-12-UTP, and Fluorescein-15-2′-dATP available from Boehringer Mannheim, Indianapolis, Ind.; and Chromosome Labeled Nucleotides, BODIPY-FL-1 4-UTP, BODIPY-FL-4-UTP, BODIPY-TMR-14-UTP, BODIPY-TMR-14-dUTP, BODIPY-TR-14-UTP, BODIPY-TR-14-dUTP, Cascade Blue-7-UTP, Cascade Blue-7-dUTP, fluorescein-12-UTP, fluorescein-12-dUTP, Oregon Green 488-5-dUTP, Rhodamine Green-5-UTP, Rhodamine Green-5-dUTP, tetramethylrhodamine-6-UTP, tetramethylrhodamine-6-dUTP, Texas Red-5-UTP, Texas Red-5-dUTP, and Texas Red-12-dUTP available from Molecular Probes, Eugene, Oreg. Nucleotides may also be labeled or marked by chemical modification. A chemically-modified single nucleotide can be biotin-dNTP. Some non-limiting examples of biotinylated dNTPs can include, biotin-dATP (e.g., bio-N6-ddATP, biotin-14-dATP), biotin-dCTP (e.g., biotin-11-cICTP, biotin-14-dCTP), and biotin-dUTP (e.g. biotin-II-dUTP, biotin-1.6-dUTP, biotin-20-dUTP). 
     The terms “polynucleotide,” “oligonucleotide,” and “nucleic acid” are used interchangeably to refer to a polymeric form of nucleotides of any length, either deoxyribonucleotides or ribonucleotides, or analogs thereof, either in single-, double-, or multi-stranded form. A polynucleotide may be exogenous or endogenous to a cell. A polynucleotide may exist in a cell-free environment. A polynucleotide may be a gene or fragment thereof. A polynucleotide may be DNA. A polynucleotide may be RNA. A polynucleotide may have any three-dimensional structure, and may perform any function, known or unknown. A polynucleotide may comprise one or more analogs (e.g. altered backbone, sugar, or nucleobase). If present, modifications to the nucleotide structure may be imparted before or after assembly of the polymer. Some non-limiting examples of modified nucleotides or analogs include: pseudouridine, 5-bromouracil, 5-methylcytosine, peptide nucleic acid, xeno nucleic acid, morpholinos, locked nucleic acids, glycol nucleic acids, threose nucleic acids, dideoxynucleotides, cordycepin, 7-deaza-GTP, florophores (e.g. rhodamine or fluorescein linked to the sugar), thiol containing nucleotides, biotin linked nucleotides, fluorescent base analogs, CpG islands, methyl-7-guanosine, methylated nucleotides, inosine, thiouridine, pseudourdine, dihydrouridine, queuosine, and wyosine. Non-limiting examples of polynucleotides include coding or non-coding regions of a gene or gene fragment, loci (locus) defined from linkage analysis, exons, introns, messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), short interfering RNA (siRNA), short-hairpin RNA (shRNA), micro-RNA (miRNA), ribozymes, eDNA, recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA of any sequence, isolated RNA of any sequence, cell-free polynucleotides including cell-free DNA (cfDNA) and cell-free RNA (cfRNA), nucleic acid probes, and primers. The sequence of nucleotides may be interrupted by non-nucleotide components. 
     In some embodiments, the nucleic acid composition may comprise one or more mRNA, comprising at least one mRNA encoding a transmembrane receptor implicated in an immune response function (e.g. a phagocytic receptor or synthetic chimeric antigen receptor) into human macrophage or dendritic cell or a suitable myeloid cell or a myeloid precursor cell. In some embodiments, the nucleic acid composition comprises one or more mRNA, and one or more lipids for delivery of the nucleic acid into a cell of hematopoietic origin, such as a myeloid cell or a myeloid cell precursor cell. In some embodiments, the one or more lipids may form a liposomal complex. 
     As used herein, the composition described herein may be used for delivery inside a cell. A cell may originate from any organism having one or more cells. Some non-limiting examples include: a prokaryotic cell, eukaryotic cell, a bacterial cell, an archaeal cell, a cell of a single-cell eukaryotic organism, a protozoa cell, a cell from a plant (e.g. cells from plant crops, fruits, vegetables, grains, soy bean, corn, maize, wheat, seeds, tomatoes, rice, cassava, sugarcane, pumpkin, hay, potatoes, cotton,  cannabis , tobacco, flowering plants, conifers, gymnosperms, ferns, clubmosses, hornworts, liverworts, mosses), an algal cell, (e.g.,  Botryococcus braunii, Chlamydomonas reinhardtii, Nannochloropsis gaditana, Chlorella pyrenoidosa, Sargassum patens  C. Agardh, and the like), seaweeds (e.g. kelp), a fungal cell (e.g., a yeast cell, a cell from a mushroom), an animal cell, a cell from an invertebrate animal (e.g. fruit fly, cnidarian, echinoderm, nematode, etc.), a cell from a vertebrate animal (e.g., fish, amphibian, reptile, bird, mammal), a cell from a mammal (e.g., a pig, a cow, a goat, a sheep, a rodent, a rat, a mouse, a non-human primate, a human, etc.), and etcetera. Sometimes a cell may not be originating from a natural organism (e.g. a cell may be a synthetically made, sometimes termed an artificial cell). In some embodiments, the cell referred to herein is a mammalian cell. In some embodiments, the cell is a human cell. The methods and compositions described herein relates to incorporating a genetic material in a cell, more specifically a human cell, wherein the human cell can be any human cell. As used herein, a human cell may be of any origin, for example, a somatic cell, a neuron, a fibroblast, a muscle cell, an epithelial cell, a cardiac cell, or a hematopoietic cell. The methods and compositions described herein can also be applicable to and useful for incorporating exogenous nucleic acid in hard-to-transfect human cell. The methods are simple and universally applicable once a suitable exogenous nucleic acid construct has been designed and developed. The methods and compositions described herein are applicable to incorporate an exogenous nucleic acid in a cell ex vivo. In some embodiments, the compositions may be applicable for systemic administration in an organism, where the nucleic acid material in the composition may be taken up by a cell in vivo, whereupon it is incorporated in cell in vivo. 
     In some embodiments, the methods and compositions described herein may be directed to incorporating an exogenous nucleic acid in a human hematopoietic cell, for example, a human cell of hematopoietic origin, such as a human myeloid cell or a myeloid cell precursor. However, the methods and compositions described herein can be used or made suitable for use in any biological cell with minimum modifications. Therefore, a cell as may refer to any cell that is a basic structural, functional and/or biological unit of a living organism. 
     In one aspect, provided herein are methods and compositions for utilizing transposable elements for stable incorporation of one or more nucleic acids into the genome of a cell, where the cell is a member of a hematopoietic cells, for example a myeloid cell. In some embodiments, the one or more nucleic acids comprise at least one nucleic acid sequence encoding a transmembrane receptor protein having a role in immune response. In some embodiments, the methods and compositions are directed to using a retrotransposable element for incorporating one or more nucleic acid sequences into a myeloid cell. The nucleic acid composition may comprise one or more nucleic sequences, such as a gene, where the gene is a transgene. The term “gene,” as used herein, refers to a nucleic acid (e.g., DNA such as genomic DNA and cDNA) and its corresponding nucleotide sequence that is involved in encoding an RNA transcript. The term as used herein with reference to genomic DNA includes intervening, non-coding regions as well as regulatory regions and may include 5′ and 3′ ends. In some uses, the term encompasses the transcribed sequences, including 5′ and 3′ untranslated regions (5′-UTR and 3′-UTR), exons and introns. In some genes, the transcribed region will contain “open reading frames” that encode polypeptides. In some uses of the term, a “gene” comprises only the coding sequences (e.g., an “open reading frame” or “coding region”) necessary for encoding a polypeptide. In some cases, genes do not encode a polypeptide, for example, ribosomal RNA genes (rRNA) and transfer RNA (tRNA) genes. In some cases, the term “gene” includes not only the transcribed sequences, but in addition, also includes non-transcribed regions including upstream and downstream regulatory regions, enhancers and promoters. A gene may refer to an “endogenous gene” or a native gene in its natural location in the genome of an organism. A gene may refer to an “exogenous gene” or a non-native gene. A non-native gene may refer to a gene not normally found in the host organism, but which is introduced into the host organism by gene transfer. A non-native gene may also refer to a gene not in its natural location in the genome of an organism. A non-native gene may also refer to a naturally occurring nucleic acid or polypeptide sequence that comprises mutations, insertions and/or deletions (e.g., non-native sequence). 
     The term “transgene” refers to any nucleic acid molecule that is introduced into a cell, that may be intermittently termed herein as a recipient cell. The resultant cell after receiving a transgene may be referred to a transgenic cell. A transgene may include a gene that is partly or entirely heterologous (i.e., foreign) to the transgenic organism or cell, or may represent a gene homologous to an endogenous gene of the organism or cell. In some cases, transgenes include any polynucleotide, such as a gene that encodes a polypeptide or protein, a polynucleotide that is transcribed into an inhibitory polynucleotide, or a polynucleotide that is not transcribed (e.g., lacks an expression control element, such as a promoter that drives transcription). Transcripts and encoded polypeptides may be collectively referred to as “gene product.” If the polynucleotide is derived from genomic DNA, expression may include splicing of the mRNA in a eukaryotic cell. “Up-regulated,” with reference to expression, refers to an increased expression level of a polynucleotide (e.g., RNA such as mRNA) and/or polypeptide sequence relative to its expression level in a wild-type state while “down-regulated” refers to a decreased expression level of a polynucleotide (e.g., RNA such as mRNA) and/or polypeptide sequence relative to its expression in a wild-type state. Expression of a transfected gene may occur transiently or stably in a cell. During “transient expression” the transfected gene is not transferred to the daughter cell during cell division. Since its expression is restricted to the transfected cell, expression of the gene is lost over time. In contrast, stable expression of a transfected gene may occur when the gene is co-transfected with another gene that confers a selection advantage to the transfected cell. Such a selection advantage may be a resistance towards a certain toxin that is presented to the cell. Where a transfected gene is required to be expressed, the application envisages the use of codon-optimized sequences. An example of a codon optimized sequence may be a sequence optimized for expression in a eukaryote, e.g., humans (i.e. being optimized for expression in humans), or for another eukaryote, animal or mammal. Codon optimization for a host species other than human, or for codon optimization for specific organs is known. In some embodiments, the coding sequence encoding a protein may be codon optimized for expression in particular cells, such as eukaryotic cells. The eukaryotic cells may be those of or derived from a particular organism, such as a plant or a mammal, including but not limited to human, or non-human eukaryote or animal or mammal as herein discussed, e.g., mouse, rat, rabbit, dog, livestock, or non-human mammal or primate. Codon optimization refers to a process of modifying a nucleic acid sequence for enhanced expression in the host cells of interest by replacing at least one codon (e.g., about or more than about 1, 2, 3, 4, 5, 10, 15, 20, 25, 50, or more codons) of the native sequence with codons that are more frequently or most frequently used in the genes of that host cell while maintaining the native amino acid sequence. Various species exhibit particular bias for certain codons of a particular amino acid. Codon bias (differences in codon usage between organisms) often correlates with the efficiency of translation of messenger RNA (mRNA), which is in turn believed to be dependent on, among other things, the properties of the codons being translated and the availability of particular transfer RNA (tRNA) molecules. The predominance of selected tRNAs in a cell may generally reflect the codons used most frequently in peptide synthesis. Accordingly, genes may be tailored for optimal gene expression in a given organism based on codon optimization. Codon usage tables are readily available, for example, at the “Codon Usage Database” available at www.kazusa.orjp/codon/ and these tables may be adapted in a number of ways. Computer algorithms for codon optimizing a particular sequence for expression in a particular host cell are also available, such as Gene Forge (Aptagen; Jacobus, Pa.), are also available. 
     A “multicistronic transcript” as used herein refers to an mRNA molecule that contains more than one protein coding region, or cistron. A mRNA comprising two coding regions is denoted a “bicistronic transcript.” The “5′-proximal” coding region or cistron is the coding region whose translation initiation codon (usually AUG) is closest to the 5′ end of a multicistronic mRNA molecule. A “5′-distal” coding region or cistron is one whose translation initiation codon (usually AUG) is not the closest initiation codon to the 5′ end of the mRNA. 
     The terms “transfection” or “transfected” refer to introduction of a nucleic acid into a cell by non-viral or viral-based methods. The nucleic acid molecules may be gene sequences encoding complete proteins or functional portions thereof. See, e.g., Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, 18.1-18.88. 
     The term “promoter,” as used herein, refers to a polynucleotide sequence capable of driving transcription of a coding sequence in a cell. Thus, promoters used in the polynucleotide constructs of the disclosure include cis-acting transcriptional control elements and regulatory sequences that are involved in regulating or modulating the timing and/or rate of transcription of a gene. For example, a promoter may be a cis-acting transcriptional control element, including an enhancer, a promoter, a transcription terminator, an origin of replication, a chromosomal integration sequence, 5′ and 3′ untranslated regions, or an intronic sequence, which are involved in transcriptional regulation. These cis-acting sequences typically interact with proteins or other biomolecules to carry out (turn on/off, regulate, modulate, etc.) gene transcription. A “constitutive promoter” is one that is capable of initiating transcription in nearly all tissue types, whereas a “tissue-specific promoter” initiates transcription only in one or a few particular tissue types. An “inducible promoter” is one that initiates transcription only under particular environmental conditions, developmental conditions, or drug or chemical conditions. Exemplary inducible promoter may be a doxycycline or a tetracycline inducible promoter. Tetracycline regulated promoters may be both tetracycline inducible or tetracycline repressible, called the tet-on and tet-off systems. The tet regulated systems rely on two components, i.e., a tetracycline-controlled regulator (also referred to as transactivator) (tTA or rtTA) and a tTA/rtTA-dependent promoter that controls expression of a downstream cDNA, in a tetracycline-dependent manner. tTA is a fusion protein containing the repressor of the Tn10 tetracycline-resistance operon of  Escherichia coli  and a carboxyl-terminal portion of protein 16 of herpes simplex virus (VP16). The tTA-dependent promoter consists of a minimal RNA polymerase II promoter fused to tet operator (tetO) sequences (an array of seven cognate operator sequences). This fusion converts the tet repressor into a strong transcriptional activator in eukaryotic cells. In the absence of tetracycline or its derivatives (such as doxycycline), tTA binds to the tetO sequences, allowing transcriptional activation of the tTA-dependent promoter. However, in the presence of doxycycline, tTA cannot interact with its target and transcription does not occur. The tet system that uses tTA is termed tet-OFF, because tetracycline or doxycycline allows transcriptional down-regulation. In contrast, in the tet-ON system, a mutant form of tTA, termed rtTA, has been isolated using random mutagenesis. In contrast to tTA, rtTA is not functional in the absence of doxycycline but requires the presence of the ligand for transactivation. The term “exon” refers to a nucleic acid sequence found in genomic DNA that is bioinformatically predicted and/or experimentally confirmed to contribute contiguous sequence to a mature mRNA transcript. The term “intron” refers to a sequence present in genomic DNA that is bioinformatically predicted and/or experimentally confirmed to not encode part of or all of an expressed protein, and which, in endogenous conditions, is transcribed into RNA (e.g. pre-mRNA) molecules, but which is spliced out of the endogenous RNA (e.g. the pre-mRNA) before the RNA is translated into a protein. 
     The term “splice acceptor site” refers to a sequence present in genomic DNA that is bioinformatically predicted and/or experimentally confirmed to be the acceptor site during splicing of pre-mRNA, which may include identified and unidentified natural and artificially derived or derivable splice acceptor sites. 
     An “internal ribosome entry site” or “IRES” refers to a nucleotide sequence that allows for 5′-end/cap-independent initiation of translation and thereby raises the possibility to express 2 proteins from a single messenger RNA (mRNA) molecule. IRESs are commonly located in the 5′ UTR of positive-stranded RNA viruses with uncapped genomes. Another means to express 2 proteins from a single mRNA molecule is by insertion of a 2A peptide(-like) sequence in between their coding sequence. 2A peptide(-like) sequences mediate self-processing of primary translation products by a process variously referred to as “ribosome skipping”, “stop-go” translation and “stop carry-on” translation. 2A peptide(-like) sequences are present in various groups of positive- and double-stranded RNA viruses including Picornaviridae, Flaviviridae, Tetraviridae, Dicistroviridae, Reoviridae and Totiviridae. 
     The term “2A peptide” refers to a class of 18-22 amino-acid (AA)-long viral oligopeptides that mediate “cleavage” of polypeptides during translation in eukaryotic cells. The designation “2A” refers to a specific region of the viral genome and different viral 2As have generally been named after the virus they were derived from. The first discovered 2A was F2A (foot-and-mouth disease virus), after which E2A (equine rhinitis A virus), P2A (porcine teschovirus-1 2A), and T2A (thosea asigna virus 2A) were also identified. The mechanism of 2A-mediated “self-cleavage” is believed to be ribosome skipping the formation of a glycyl-prolyl peptide bond at the C-terminus of the 2A sequence. 2A peptide(-like) sequences mediate self-processing of primary translation products by a process variously referred to as “ribosome skipping”, “stop-go” translation and “stop carry-on” translation. 2A peptide(-like) sequences are present in various groups of positive- and double-stranded RNA viruses including Picornaviridae, Flaviviridae, Tetraviridae, Dicistroviridae, Reoviridae and Totiviridae. 
     As used herein, the term “operably linked” refers to a functional relationship between two or more segments, such as nucleic acid segments or polypeptide segments. Typically, it refers to the functional relationship of a transcriptional regulatory sequence to a transcribed sequence. 
     The term “termination sequence” refers to a nucleic acid sequence which is recognized by the polymerase of a host cell and results in the termination of transcription. The termination sequence is a sequence of DNA that, at the 3′ end of a natural or synthetic gene, provides for termination of mRNA transcription or both mRNA transcription and ribosomal translation of an upstream open reading frame. Prokaryotic termination sequences commonly comprise a GC-rich region that has a two-fold symmetry followed by an AT-rich sequence. A commonly used termination sequence is the T7 termination sequence. A variety of termination sequences are known in the art and may be employed in the nucleic acid constructs of the present invention, including the TINT3, TL13, TL2, TR1, TR2, and T6S termination signals derived from the bacteriophage lambda, and termination signals derived from bacterial genes, such as the trp gene of  E. coli.    
     The terms “polyadenylation sequence” (also referred to as a “poly A site” or “poly A sequence”) refers to a DNA sequence which directs both the termination and polyadenylation of the nascent RNA transcript. Efficient polyadenylation of the recombinant transcript is desirable, as transcripts lacking a poly A tail are typically unstable and rapidly degraded. The poly A signal utilized in an expression vector may be “heterologous” or “endogenous”. An endogenous poly A signal is one that is found naturally at the 3′ end of the coding region of a given gene in the genome. A heterologous poly A signal is one which is isolated from one gene and placed 3′ of another gene, e.g., coding sequence for a protein. A commonly used heterologous poly A signal is the SV40 poly A signal. The SV40 poly A signal is contained on a 237 bp BamHI/BclI restriction fragment and directs both termination and polyadenylation; numerous vectors contain the SV40 poly A signal. Another commonly used heterologous poly A signal is derived from the bovine growth hormone (BGH) gene; the BGH poly A signal is also available on a number of commercially available vectors. The poly A signal from the Herpes simplex virus thymidine kinase (HSV tk) gene is also used as a poly A signal on a number of commercial expression vectors. The polyadenylation signal facilitates the transportation of the RNA from within the cell nucleus into the cytosol as well as increases cellular half-life of such an RNA. The polyadenylation signal is present at the 3′-end of an mRNA. 
     The terms “complement,” “complements,” “complementary,” and “complementarity,” as used herein, refer to a sequence that is complementary to and hybridizable to the given sequence. In some cases, a sequence hybridized with a given nucleic acid is referred to as the “complement” or “reverse-complement” of the given molecule if its sequence of bases over a given region is capable of complementarily binding those of its binding partner, such that, for example, A-T, A-U, G-C, and G-U base pairs are formed. In general, a first sequence that is hybridizable to a second sequence is specifically or selectively hybridizable to the second sequence, such that hybridization to the second sequence or set of second sequences is preferred (e.g. thermodynamically more stable under a given set of conditions, such as stringent conditions commonly used in the art) to hybridization with non-target sequences during a hybridization reaction. Typically, hybridizable sequences share a degree of sequence complementarity over all or a portion of their respective lengths, such as between 25%-100% complementarity, including at least 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, and 100% sequence complementarity. Sequence identity, such as for the purpose of assessing percent complementarity, may be measured by any suitable alignment algorithm, including but not limited to the Needleman-Wunsch algorithm (see e.g. the EMBOSS Needle aligner available at www.ebi.ac.uk/Tools/psa/embossneedle/nucleotide.html), the BLAST algorithm (see e.g. the BLAST alignment tool available at blast.ncbi.nlm.nih.gov/Blast.cgi, optionally with default settings), or the Smith-Waterman algorithm (see e.g. the EMBOSS Water aligner available at www.ebi.ac.ukaools/psa/emboss_water/nucleotide.html, optionally with default settings). Optimal alignment can be assessed using any suitable parameters of a chosen algorithm, including default parameters. 
     Complementarity may be perfect or substantial/sufficient. Perfect complementarity between two nucleic acids may mean that the two nucleic acids may form a duplex in which every base in the duplex is bonded to a complementary base by Watson-Crick pairing. Substantial or sufficient complementary may mean that, a sequence in one strand is not completely and/or perfectly complementary to a sequence in an opposing strand, but that sufficient bonding occurs between bases on the two strands to form a stable hybrid complex in set of hybridization conditions (e.g., salt concentration and temperature). Such conditions may be predicted by using the sequences and standard mathematical calculations to predict the melting temperature (T m ) of hybridized strands, or by empirical determination of T m  by using routine methods. 
     “Transposons” as used herein are segments within the chromosome that can translocate within the genome, also known as “jumping gene”. There are two different classes of transposons: class 1, or retrotransposons, that mobilize via an RNA intermediate and a “copy-and-paste” mechanism, and class II, or DNA transposons, that mobilize via excision integration, or a “cut-and-paste” mechanism (Ivics Nat Methods 2009). Bacterial, lower eukaryotic (e.g. yeast) and invertebrate transposons appear to be largely species specific, and cannot be used for efficient transposition of DNA in vertebrate cells. “Sleeping Beauty” (Ivics Cell 1997), was the first active transposon that was artificially reconstructed by sequence shuffling of inactive TEs from fish. This made it possible to successfully achieve DNA integration by transposition into vertebrate cells, including human cells. Sleeping Beauty is a class II DNA transposon belonging to the Tcl/mariner family of transposons (Ni Genomics Proteomics 2008). In the meantime, additional functional transposons have been identified or reconstructed from different species, including  Drosophila , frog and even human genomes, that all have been shown to allow DNA transposition into vertebrate and also human host cell genomes. Each of these transposons have advantages and disadvantages that are related to transposition efficiency, stability of expression, genetic payload capacity etc. Exemplary class II transposases that have been created include Sleeping Beauty, PiggyBac, Frog Prince, Himarl, Passport, Minos, hAT, Toll, To12, AciDs, PIF, Harbinger, Harbinger3-DR, and Hsmarl. 
     “Heterologous” as used herein, includes molecules such as DNA and RNA which may not naturally be found in the cell into which it is inserted. For example, when mouse or bacterial DNA is inserted into the genome of a human cell, such DNA is referred to herein as heterologous DNA. In contrast, the term “homologous” as used herein, denotes molecules such as DNA and RNA that are found naturally in the cell into which it is inserted. For example, the insertion of mouse DNA into the genome of a mouse cell constitutes insertion of homologous DNA into that cell. In the latter case, it is not necessary that the homologous DNA be inserted into a site in the cell genome in which it is naturally found; rather, homologous DNA may be inserted at sites other than where it is naturally found, thereby creating a genetic alteration (a mutation) in the inserted site. 
     A “transposase” is an enzyme that is capable of forming a functional complex with a transposon end-containing composition (e.g., transposons, transposon ends), and catalyze insertion or transposition of the transposon end-containing composition into double stranded DNA which is incubated with an in vitro transposon reaction. The term “transposon end” means a double-stranded DNA that contains the nucleotide sequences (the “transposon end sequences”) necessary to form the complex with the transposase or integrase enzyme that is functional in an in vitro transposition reaction. 
     A transposon end forms a complex or a synaptic complex or a transposon complex or a transposon composition with a transposase or integrase that recognizes and binds to the transposon end, and which complex is capable of inserting or transposing the transposon end into target DNA with which it is incubated in an in vitro transposition reaction. A transposon end exhibits two complementary sequences consisting of a transferred transposon end sequence or transferred strand and a non-transferred transposon end sequence, or non-transferred strand For example, one transposon end that forms a complex with a hyperactive Tn5 transposase that is active in an in vitro transposition reaction comprises a transferred strand that exhibits a transferred transposon end sequence as follows: 5′ AGATGTGTATAAGAGACAG 3′ (SEQ ID NO: 55), and a non-transferred strand that exhibits a “non-transferred transposon end sequence” as follows: (5′ CTGTCTCTTATACACATCT 3′ (SEQ ID NO: 56). The 3′-end of a transferred strand is joined or transferred to target DNA in an in vitro transposition reaction. The non-transferred strand, which exhibits a transposon end sequence that is complementary to the transferred transposon end sequence, is not joined or transferred to the target DNA in an in vitro transposition reaction. 
     In some embodiments, the transferred strand and non-transferred strand are covalently joined. For example, in some embodiments, the transferred and non-transferred strand sequences are provided on a single oligonucleotide, e.g., in a hairpin configuration. As such, although the free end of the non-transferred strand is not joined to the target DNA directly by the transposition reaction, the non-transferred strand becomes attached to the DNA fragment indirectly, because the non-transferred strand is linked to the transferred strand by the loop of the hairpin structure. As used herein an “cleavage domain” refers to a nucleic acid sequence that is susceptible to cleavage by an agent, e.g., an enzyme. 
     A “restriction site domain” means a tag domain that exhibits a sequence for the purpose of facilitating cleavage using a restriction endonuclease. For example, in some embodiments, the restriction site domain is used to generate di-tagged linear ssDNA fragments. In some embodiments, the restriction site domain is used to generate a compatible double-stranded 5′-end in the tag domain so that this end can be ligated to another DNA molecule using a template-dependent DNA ligase. In some embodiments, the restriction site domain in the tag exhibits the sequence of a restriction site that is present only rarely, if at all, in the target DNA (e.g., a restriction site for a rare-cutting restriction endonuclease such as NotI or AscI). 
     As used herein, the term “recombinant nucleic acid molecule” refers to a recombinant DNA molecule or a recombinant RNA molecule. A recombinant nucleic acid molecule is any nucleic acid molecule containing joined nucleic acid molecules from different original sources and not naturally attached together. Recombinant RNA molecules include RNA molecules transcribed from recombinant DNA molecules. A recombinant nucleic acid may be synthesized in the laboratory. A recombinant nucleic acid can be prepared by using recombinant DNA technology by using enzymatic modification of DNA, such as enzymatic restriction digestion, ligation, and DNA cloning. A recombinant DNA may be transcribed in vitro, to generate a messenger RNA (mRNA), the recombinant mRNA may be isolated, purified and used to transfect a cell. A recombinant nucleic acid may encode a protein or a polypeptide. A recombinant nucleic acid, under suitable conditions, can be incorporated into a living cell, and can be expressed inside the living cell. As used herein, “expression” of a nucleic acid usually refers to transcription and/or translation of the nucleic acid. The product of a nucleic acid expression is usually a protein but can also be an mRNA. Detection of an mRNA encoded by a recombinant nucleic acid in a cell that has incorporated the recombinant nucleic acid, is considered positive proof that the nucleic acid is “expressed” in the cell. The process of inserting or incorporating a nucleic acid into a cell can be via transformation, transfection or transduction. Transformation is the process of uptake of foreign nucleic acid by a bacterial cell. This process is adapted for propagation of plasmid DNA, protein production, and other applications. Transformation introduces recombinant plasmid DNA into competent bacterial cells that take up extracellular DNA from the environment. Some bacterial species are naturally competent under certain environmental conditions, but competence is artificially induced in a laboratory setting. Transfection is the forced introduction of small molecules such as DNA, RNA, or antibodies into eukaryotic cells. Just to make life confusing, ‘transfection’ also refers to the introduction of bacteriophage into bacterial cells. ‘Transduction’ is mostly used to describe the introduction of recombinant viral vector particles into target cells, while ‘infection’ refers to natural infections of humans or animals with wild-type viruses. 
     A “stem-loop” sequence refers to a nucleic acid sequence (e.g., RNA sequence) with sufficient self-complementarity to hybridize and form a stem and the regions of non-complementarity that bulges into a loop. The stem may comprise mismatches or bulges. 
     The term “vector” refers to a nucleic acid molecule capable of transporting or mediating expression of a heterologous nucleic acid. A “vector sequence” as used herein, refers to a sequence of nucleic acid comprising at least one origin of replication and at least one selectable marker gene. Vectors capable of directing the expression of genes and/or nucleic acid sequence to which they are operatively linked are referred to herein as “expression vectors”. 
     A plasmid is a species of the genus encompassed by the term “vector.” In general, expression vectors of utility are often in the form of “plasmids” which refer to circular double stranded DNA molecules which, in their vector form are not bound to the chromosome, and typically comprise entities for stable or transient expression of the encoded DNA. Other expression vectors that can be used in the methods as disclosed herein include, but are not limited to plasmids, episomes, bacterial artificial chromosomes, yeast artificial chromosomes, bacteriophages or viral vectors, and such vectors can integrate into the host&#39;s genome or replicate autonomously in the cell. A vector can be a DNA or RNA vector. Other forms of expression vectors known by those skilled in the art which serve the equivalent functions can also be used, for example, self-replicating extrachromosomal vectors or vectors capable of integrating into a host genome. Exemplary vectors are those capable of autonomous replication and/or expression of nucleic acids to which they are linked. A safe harbor locus is a region within the genome where additional exogenous or heterologous nucleic acid sequence can be inserted, and the host genome is able to accommodate the inserted genetic material. Exemplary safe harbor sites include but are not limited to: AAVS1 site, GGTA1 site, CMAH site, B4GALNT2 site, B2M site, ROSA26 site, COLA1 site, and TIGRE site. For example, the heterologous nucleic acid described in this disclosure may be integrated at one or more sites in the genome of the cell, wherein the one or more locations is selected from the group consisting of: AAVS1 site, GGTA1 site, CMAH site, B4GALNT2 site, B2M site, ROSA26 site, COLA1 site, and TIGRE site. In some embodiments, the nucleic acid cargo comprising the transgene may be delivered to a R2D locus. 
     In some embodiments, the nucleic acid cargo comprising the transgene may be delivered to the genome in an intergenic or intragenic region. In some embodiments the nucleic acid cargo comprising the transgene is integrated into the genome 5′ or 3′ within 0.1 kb, 0.25 kb, 0.5 kb, 0.75, kb, 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 7.5 kb, 10 kb, 15 kb, 20 kb, 25 kb, 50, 75 kb, or 100 kb of an endogenous active gene. In some embodiments the nucleic acid cargo comprising the transgene is integrated into the genome 5′ or 3′ within 0.1 kb, 0.25 kb, 0.5 kb, 0.75, kb, 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 7.5 kb, 10 kb, 15 kb, 20 kb, 25 kb, 50, 75 kb, or 100 kb of an endogenous promoter or enhancer. In some embodiments the nucleic acid cargo comprising the transgene is 50-50,000 base pairs, e.g., between 50-40,000 bp, between 500-30,000 bp between 500-20,000 bp, between 100-15,000 bp, between 500-10,000 bp, between 50-10,000 bp, between 50-5,000 bp. In some embodiments the nucleic acid cargo comprising the transgene is less than 1,000, 1,300, 1500, 2,000, 3,000, 4,000, 5,000, or 7,500 nucleotides in length. 
     L1 and Non-L1 Retrotransposon Systems 
     Retrotransposons can contain transposable elements that are active participants in reorganizing their resident genomes. Broadly, retrotransposons can refer to DNA sequences that are transcribed into RNA and translated into protein and have the ability to reverse-transcribe themselves back into DNA. Approximately 45% of the human genome is comprised of sequences that result from transposition events. Retrotransposition occasionally generates target site deletions or adds non-retrotransposon DNA to the genome by processes termed 5′- and 3′-transduction. Recombination between non-homologous retrotransposons causes deletions, duplications or rearrangements of gene sequence. Ongoing retrotransposition can generate novel splice sites, polyadenylation signals and promoters, and so builds new transcription modules. 
     Generally, retrotransposons may be grouped into two classes, the retrovirus-like LTR retrotransposons, and the non-LTR elements such as human L1 elements,  Neurospora  TAD elements (Kinsey, 1990, Genetics 126:317-326), I factors from  Drosophila  (Bucheton et al., 1984, Cell 38:153-163), and R2Bm from  Bombyx mori  (Luan et al., 1993, Cell 72: 595-605). These two types of retrotransposons are structurally different and also retrotranspose using radically different mechanisms. Exemplary, non-limiting examples of LINE-encoded polypeptides are found in GenBank Accession Nos. AAC51261, AAC51262, AAC51263, AAC51264, AAC51265, AAC51266, AAC51267, AAC51268, AAC51269, AAC51270, AAC51271, AAC51272, AAC51273, AAC51274, AAC51275, AAC51276, AAC51277, AAC51278 and AAC51279. 
     The decision to focus on LINE-1 to develop into a system as described in the disclosure for a number of reasons at least some of which are exemplified below: (a) LINE-1 (or L1-) elements are autonomous as they encode all of the machinery alone to complete this reverse transcription and integration process; (b) L1 elements are abundant in the human genome, such that these elements may be considered as a naturalized element of the genome; (c) L1 retrotransposon retrotransposes its own mRNA with high degree of specificity, compared to other mRNAs floating around in the cells. 
     The L1 expresses a 6-kb bicistronic RNA that encodes the 40 kDa Open Reading Frame-1 RNA-binding protein (ORF1p) of essential but uncertain function, and a 150 kDa ORF2 protein with endonuclease and reverse transcriptase (RT) activities. L1 retrotransposition is a complex process involving transcription of the L1, transport of its RNA to the cytoplasm, translation of the bicistronic RNA, formation of a ribonucleoprotein (RNP) particle, its re-import to the nucleus and target-primed reverse transcription at the integration site. A few transcription factors that interact with L Is have been identified. Transcribed L1 RNA forms an RNP in cis with the proteins that are translated from the transcript. L1 integrates into genomic DNA by target-site primer reverse transcription (TPRT) by ORF2p cleavage at the 5′-TTTT-3′ where a poly A sequence of L1 RNA anneals and primes reverse transcriptase (RT) activity to make L1 cDNA. 
     Other mobile elements of the genome can “hijack” the L1 ORF for retrotransposition. For example, Alu elements are such mobile DNA elements that belong to the class of short interspersed elements (SINEs) that are non-autonomous retrotransposons and acquire trans-factors to integrate. Alu elements and SINE-1 elements can associate with the L1 ribonucleoproteins in trans to be also retrotransposed by ORF1p and ORF2p. Somewhat similar to the L1 RNA, the Alu element ends with a long A-run, often referred to as the A-tail, and it also has a smaller A-rich region (indicated by AA) separating the two halves of a diverged dimer structure. Alu elements are likely to have the internal components of an RNA polymerase III promoter (such as, commonly designated as an A box and a B box promoters), but they do not encode a terminator for RNA polymerase III. They may utilize a stretch of T nucleotides at various distances downstream of the Alu element to terminate a transcription. A typical Alu transcript encompasses the entire Alu, including the A-tail, and has a 3′ region that is unique for each locus. The Alu RNA folds into separate structures for each monomer unit. The RNA has been shown to bind the 7SL RNA SRP9 and 14 heterodimer, as well as poly A-binding protein (PABP). The poly A tail of Alu primes with T rich (TTTT) region of the genome and attracts ORF2p to bind to the primed region and cleaves at the T rich region via its endonuclease activity. The T-rich region primes reverse transcription by ORF2p on the 3′ A-tail region of the Alu element. This creates a cDNA copy of the body of the Alu element. A nick occurs by an unknown mechanism on the second strand and second-strand synthesis is primed. The new Alu element is then flanked by short direct repeats that are duplicates of the DNA sequence between the first and second nicks. Alu elements are extremely prevalent within RNA molecules, owing to their preference for gene-rich regions. A full-length Alu (˜300 bp) is derived from the signal recognition particle RNA 7SL and consists of two similar monomers with an A-rich linker in-between, A- and B-boxes present in the 5′ monomer, and a poly-A tail lacking the preceding polyadenylation signal resulting in an elongated tail (up to 100 bp in length). Alus can be transcribed by RNA polymerase III using the internal promoters within the A- and B-boxes; however, Alus contain no ORFs and therefore do not encode for protein products. 
     Other non-L1 transposons include SVAs and HERV-Ks. A full-length SVA (SINE-VNTR-Alu) element (˜2-3 kb) is a composite unit that contains a CCCTCT repeat, two Alu-like sequences, a VNTR, a SINE-R region with env (envelope) gene, the 3′ LTR of HERV-K10, and a polyadenylation signal followed by a poly-A tail. It is most likely that SVAs are transcribed by RNA polymerase II, although it is unknown whether SVA elements carry an internal promoter. 
     A full-length HERV-K element (˜9-10 kb) is comprised of ancient remnants of endogenous retroviral sequences and includes two flanking LTR regions surrounding three retroviral ORFs: (1) gag encoding the structural proteins of a retroviral capsid; (2) pol-pro encoding the enzymes: protease, RT, and integrase; and (3) env encoding proteins allowing for horizontal transfer. The LTR of HERV-K contains an internal, bidirectional promoter that appears to be under the transcriptional control of RNA polymerase II. 
     L1 retrotransposition and RNA binding can take place at or near poly-A tail. The 3′-UTR plays a role in the recognition of stringent-type LINE RNA of ORF1 protein (ORF1p). Stringent-type LINEs can contain a stem-loop structure located at the end of the 3′UTR. Branched molecules consisting of junctions between transposon 3′-end cDNA and the target DNA, as well as specific positioning of L1 RNA within ORF2 protein (ORF2p), were detected during initial stages of L1 retrotransposition in vitro. Secondary or tertiary RNA structure shared by L1 and Alu are likely to be responsible for recognition by and binding of ORF2, possibly along with a poly-A tail. In some embodiments, the stem-loop structure located downstream of the poly-A sequence correlates with cleavage intensity. 
     Mechanisms for restricting or resolving L1 integration have also evolved for the sake of maintaining genetic integrity and stability of the genome. Non-homologous end-joining repair proteins, such as XRCC1, Ku70 and DNA-PK, have been implicated in resolution of the L1 integrate at the time of insertion. In addition, the cell has evolved a number of proteins that stand against unrestricted retrotransposition, including the APOBEC3 family of cytosine deaminases, adenosine deaminase ADAR1, chromatin-remodeling factors and members of the piRNA pathway for post-transcription gene silencing that functions in the male germ line. 
     I. Compositions Comprising Nucleic Acid Constructs and Methods Involved for Stable Expression of Encoded Protein 
     Provided herein is a recombinant nucleic acid encoding one or more proteins for expression in a cell, such as a myeloid cell. In one embodiment, the recombinant nucleic acid is designed for stable expression of the one or more proteins or polypeptides encoded by the recombinant nucleic acid. In some embodiments, the stable expression is achieved by incorporation of recombinant nucleic acid within the genome of the cell. 
     It can be easily understood by one of skill in the art that the compositions and methods described herein can be utilized to design products in which the recombinant nucleic acid may comprise one or more sequences that do not translate as a protein or a polypeptide component, but may encode an oligonucleotide that can be a regulatory nucleic acid, such as an inhibitor oligonucleotide product, such as an activator oligonucleotide. 
     In one aspect, provided herein is a composition comprising a synthetic nucleic acid, comprising a nucleic acid sequence encoding a gene of interest and one or more retrotransposable elements to stably incorporate a non-endogenous nucleic acid into a cell. In some embodiments, the cell is a hematopoietic cell. In some embodiments, the cell is a myeloid cell. In some embodiments, the cell is a precursor cell. In some embodiments, the cell is undifferentiated. In some embodiments, the cell has further differentiation potential. In some embodiments, the cell is not a stem cell. 
     A. LINE/Alu Retrotransposon Construct 
     In some embodiments, the present disclosure may utilize a retrotransposable system to stably incorporate into the genome and express a non-endogenous nucleic acid, where the non-endogenous nucleic acid comprises retrotransposable elements within the nucleic acid sequence. In some embodiments, the present disclosure may utilize a cell&#39;s endogenous retrotransposable system (e.g., proteins and enzymes), to stably express a non-endogenous nucleic acid in the cell. In some embodiments, the present disclosure may utilize a cell&#39;s endogenous retrotransposable system (e.g., proteins and enzymes, such as a LINE1 retrotransposition system), but may further express one or more components of the retrotransposable system to stably express a non-endogenous nucleic acid in the cell. 
     In some embodiments, a synthetic nucleic acid is provided herein, the synthetic nucleic acid encoding a transgene, and encoding one or more components for retrotransposition. The synthetic nucleic acid described herein is interchangeably termed as a nucleic acid construct, transgene or the exogenous nucleic acid. 
     In one aspect, provided herein is a method of integrating a nucleic acid sequence into a genome of a cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA into the cell, wherein the mRNA comprises: an insert sequence, wherein the insert sequence comprises an exogenous sequence, or a sequence that is a reverse complement of the exogenous sequence; a 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises a binding site for a human ORF protein, and wherein the insert sequence is integrated into the genome of the cell. 
     In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a binding site for human ORF2p. 
     In one aspect, provided herein is a method for integrating a nucleic acid sequence into the genome of an immune cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA, wherein the mRNA comprises an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence or (ii) a sequence that is a reverse complement of the exogenous sequence; 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence, wherein the 5′ UTR sequence or the 3′ UTR sequence comprises an endonuclease binding site and/or a reverse transcriptase binding site, and wherein the transgene sequence is integrated into the genome of the immune cell. 
     In one aspect, provided herein is a method for integrating a nucleic acid sequence into the genome of a cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA, wherein the mRNA comprises an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence or (ii) a sequence that is a reverse complement of the exogenous sequence; a 5′ UTR sequence, a sequence of a human retrotransposon downstream of the 5′ UTR sequence, and a 3′ UTR sequence downstream of the sequence of a human retrotransposon; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises an endonuclease binding site and/or a reverse transcriptase binding site, and wherein the sequence of a human retrotransposon encodes for two proteins that are translated from a single RNA containing two ORFs, and wherein the insert sequence is integrated into the genome of the cell. 
     In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises an ORF2p binding site. In some embodiments, the ORF2p binding site is a poly A sequence in the 3′ UTR sequence. 
     In some embodiments, the mRNA comprises a sequence of a human retrotransposon. In some embodiments, the sequence of a human retrotransposon is downstream of the 5′ UTR sequence. In some embodiments, the sequence of a human retrotransposon is upstream of the 3′ UTR sequence. 
     In some embodiments, the sequence of a human retrotransposon encodes for two proteins that are translated from a single RNA containing two ORFs. In some embodiments, the two ORFs are non-overlapping ORFs. In some embodiments, the two ORFs are ORF1 and ORF2. In some embodiments, the ORF1 encodes ORF1p and ORF2 encodes ORF2p. 
     In some embodiments, the sequence of a human retrotransposon comprises a sequence of a non-LTR retrotransposon. In some embodiments, the sequence of a human retrotransposon encodes comprises a LINE-1 retrotransposon. In some embodiments, the LINE-1 retrotransposon is a human LINE-1 retrotransposon. In some embodiments, the sequence of a human retrotransposon comprises a sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the endonuclease and/or a reverse transcriptase is ORF2p. In some embodiments, the reverse transcriptase is a group II intron reverse transcriptase domain. In some embodiments, the endonuclease and/or a reverse transcriptase is a minke whale endonuclease and/or a reverse transcriptase. In some embodiments, the sequence of a human retrotransposon comprises a sequence encoding ORF2p. In some embodiments, the insert sequence is integrated into the genome at a poly T site using specificity of an endonuclease domain of the ORF2p. In some embodiments, the poly T site comprises the sequence TTTTTA. 
     In some embodiments, (i) the sequence of a human retrotransposon comprises a sequence encoding ORF1p, (ii) the mRNA does not comprise a sequence encoding ORF1p, or (iii) the mRNA comprises a replacement of the sequence encoding ORF1p with a 5′ UTR sequence from the complement gene. In some embodiments, the mRNA comprises a first mRNA molecule encoding ORF1p, and a second mRNA molecule encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the mRNA is an mRNA molecule comprising a first sequence encoding ORF1p, and a second sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the first sequence encoding ORF1p and the second sequence encoding an endonuclease and/or a reverse transcriptase are separated by a linker sequence. 
     In some embodiments, the linker sequence comprises an internal ribosome entry sequence (IRES). In some embodiments, the IRES is an IRES from CVB3 or EV71. In some embodiments, the linker sequence encodes a self-cleaving peptide sequence. In some embodiments, the linker sequence encodes a T2A, a E2A or a P2A sequence 
     In some embodiments, the sequence of a human retrotransposon comprises a sequence that encodes ORF1p fused to an additional protein sequence and/or a sequence that encodes ORF2p fused to an additional protein sequence. In some embodiments, the ORF1p and/or the ORF2p is fused to a nuclear retention sequence. In some embodiments, the nuclear retention sequence is an Alu sequence. In some embodiments, the ORF1p and/or the ORF2p is fused to an MS2 coat protein. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises at least one, two, three or more MS2 hairpin sequences. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a sequence that promotes or enhances interaction of a poly A tail of the mRNA with the endonuclease and/or a reverse transcriptase. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a sequence that promotes or enhances interaction of a poly-A-binding protein (PABP) with the endonuclease and/or a reverse transcriptase. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises a sequence that increases specificity of the endonuclease and/or a reverse transcriptase to the mRNA relative to another mRNA expressed by the cell. In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence comprises an Alu element sequence. 
     In some embodiments, the first sequence encoding ORF1p and the second sequence encoding an endonuclease and/or a reverse transcriptase have the same promoter. In some embodiments, the insert sequence has a promoter that is different from the promoter of the first sequence encoding ORF1p. In some embodiments, the insert sequence has a promoter that is different from the promoter of the second sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the first sequence encoding ORF1p and/or the second sequence encoding an endonuclease and/or a reverse transcriptase have a promoter or transcription initiation site selected from the group consisting of an inducible promoter, a CMV promoter or transcription initiation site, a T7 promoter or transcription initiation site, an EF1a promoter or transcription initiation site and combinations thereof. In some embodiments, the insert sequence has a promoter or transcription initiation site selected from the group consisting of an inducible promoter, a CMV promoter or transcription initiation site, a T7 promoter or transcription initiation site, an EF1a promoter or transcription initiation site and combinations thereof. 
     In some embodiments, the first sequence encoding ORF1p and the second sequence encoding an endonuclease and/or a reverse transcriptase are codon optimized for expression in a human cell. 
     In some embodiments, the mRNA comprises a WPRE element. In some embodiments, the mRNA comprises a selection marker. In some embodiments, the mRNA comprises a sequence encoding an affinity tag. In some embodiments, the affinity tag is linked to the sequence encoding an endonuclease and/or a reverse transcriptase. 
     In some embodiments, the 3′ UTR comprises a poly A sequence or wherein a poly A sequence is added to the mRNA in vitro. In some embodiments, the poly A sequence is downstream of a sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the insert sequence is upstream of the poly A sequence. 
     In some embodiments, the 3′ UTR sequence comprises the insert sequence. In some embodiments, the insert sequence comprises a sequence that is a reverse complement of the sequence encoding the exogenous polypeptide. In some embodiments, the insert sequence comprises a polyadenylation site. In some embodiments, the insert sequence comprises an SV40 polyadenylation site. In some embodiments, the insert sequence comprises a polyadenylation site upstream of the sequence that is a reverse complement of the sequence encoding the exogenous polypeptide. In some embodiments, the insert sequence is integrated into the genome at a locus that is not a ribosomal locus. In some embodiments, the insert sequence integrates into a gene or regulatory region of a gene, thereby disrupting the gene or downregulating expression of the gene. In some embodiments, the insert sequence integrates into a gene or regulatory region of a gene, thereby upregulating expression of the gene. In some embodiments, the insert sequence integrates into the genome and replaces a gene. In some embodiments, the insert sequence is stably integrated into the genome. In some embodiments, the insert sequence is retrotransposed into the genome. In some embodiments, the insert sequence is integrated into the genome by cleavage of a DNA strand of a target site by an endonuclease encoded by the mRNA. In some embodiments, the insert sequence is integrated into the genome via target-primed reverse transcription (TPRT). In some embodiments, the insert sequence is integrated into the genome via reverse splicing of the mRNA into a DNA target site of the genome. 
     In some embodiments, the cell is an immune cell. In some embodiments, the immune cell is a T cell or a B cell. In some embodiments, the immune cell is a myeloid cell. In some embodiments, the immune cell is selected from a group consisting of a monocyte, a macrophage, a dendritic cell, a dendritic precursor cell, and a macrophage precursor cell. 
     In some embodiments, the mRNA is a self-integrating mRNA. In some embodiments, the method comprises introducing into the cell the mRNA. In some embodiments, the method comprises introducing into the cell the vector encoding the mRNA. In some embodiments, the method comprises introducing the mRNA or the vector encoding the mRNA into a cell ex vivo. In some embodiments, the method further comprises administering the cell to a human subject. In some embodiments, the method comprises administering the mRNA or the vector encoding the mRNA to a human subject. In some embodiments, an immune response is not elicited in the human subject. In some embodiments, the mRNA or the vector is substantially non-immunogenic. 
     In some embodiments, the vector is a plasmid or a viral vector. In some embodiments, the vector comprises a non-LTR retrotransposon. In some embodiments, the vector comprises a human L1 element. In some embodiments, the vector comprises a L1 retrotransposon ORF1 gene. In some embodiments, the vector comprises a L1 retrotransposon ORF2 gene. In some embodiments, the vector comprises a L1 retrotransposon. 
     In some embodiments, the mRNA is at least about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, or 3 kilobases. In some embodiments, the mRNA is a most about 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9 or 5 kilobases. 
     In some embodiments, the mRNA comprises a payload that is at least about 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, or 3 kilobases. In some embodiments, the mRNA is a most about 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9 or 5 kilobases. In some embodiments, the mRNA is at least about 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9 or 6 kilobases. In some embodiments, the mRNA is at least about 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9 or 7 kilobases. In some embodiments, the mRNA is at least about 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9 or 8 kilobases. In some embodiments, the mRNA is at least about 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9 or 9 kilobases. In some embodiments, the mRNA is at least about 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9 or 10 kilobases. In some embodiments, the mRNA is at least about 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9 or 11 kilobases. In some embodiments, the mRNA is at least about 11.1, 11.2, 11.3, 11.4, 11.5, 11.6, 11.7, 11.8, 11.9 or 12 kilobases. In some embodiments, the mRNA comprises a payload of about 6.8 kB, e.g., a sequence encoding a ABCA4 gene product. In some embodiments, the mRNA comprises a payload of about 6.7 kB, e.g., a sequence encoding a MY07A gene product. In some embodiments, the mRNA comprises a payload of about 7.5 kB, e.g., a sequence encoding a CEP290 gene product. In some embodiments, the mRNA comprises a payload of about 10.1 kB, e.g., a sequence encoding a CDH23 gene product. In some embodiments, the mRNA comprises a payload of about 9.4 kB, e.g., a sequence encoding a EYS gene product. In some embodiments, the mRNA comprises a payload of about 15.6 kB, e.g., a sequence encoding a USH2a gene product. In some embodiments, the mRNA comprises a payload of about 12.5 kB, e.g., a sequence encoding a ALMS1 gene product. In some embodiments, the mRNA comprises a payload of about 4.6 kB, e.g., a sequence encoding a GDE gene product. In some embodiments, the mRNA comprises a payload of about 6 kB, e.g., a sequence encoding the OTOF gene product. In some embodiments, the mRNA comprises a payload of about 7.1 kB, e.g., a sequence encoding a F8 gene product. 
     One of the advantages of using the method of integration of a nucleic acid into the genome using retrotransposition is that it can be designed as described herein to deliver a nucleic acid cargo that is much larger than that using any other existing methods. For example, lentiviral and adeno-associated viral (AAV) gene delivery method are not expected to deliver a nucleic acid cargo of greater than 4 kB. In addition, lentiviral delivery entails risk of insertional mutagenesis and other toxicities. AAV mediated delivery entails unresolved liver and CNS toxicity. On the other hand, retrotransposition mediated method (Retro-T) using mRNA as described herein is rapid, safer and less complex than these viral methods. 
     In some embodiments, the mRNA comprises a sequence that inhibits or prevents degradation of the mRNA. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA inhibits or prevents degradation of the mRNA by an exonuclease or an RNAse. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA is a G quadruplex, pseudoknot or triplex sequence. In some embodiments, the sequence the sequence that inhibits or prevents degradation of the mRNA is an exoribonuclease-resistant RNA structure from a flavivirus RNA or an ENE element from KSV. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA inhibits or prevents degradation of the mRNA by a deadenylase. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA comprises non-adenosine nucleotides within or at a terminus of a poly A tail of the mRNA. In some embodiments, the sequence that inhibits or prevents degradation of the mRNA increases stability of the mRNA. In some embodiments, the exogenous sequence comprises a sequence encoding an exogenous polypeptide. In some embodiments, the sequence encoding an exogenous polypeptide is not in frame with a sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the sequence encoding an exogenous polypeptide is not in frame with a sequence encoding an endonuclease and/or a reverse transcriptase. In some embodiments, the exogenous sequence does not comprise introns. In some embodiments, the exogenous sequence comprises a sequence encoding an exogenous polypeptide selected from the group consisting of an enzyme, a receptor, a transport protein, a structural protein, a hormone, an antibody, a contractile protein and a storage protein. In some embodiments, the exogenous sequence comprises a sequence encoding an exogenous polypeptide selected from the group consisting of a chimeric antigen receptor (CAR), a ligand, an antibody, a receptor, and an enzyme. In some embodiments, the exogenous sequence comprises a regulatory sequence. In some embodiments, the regulatory sequence comprises a cis-acting regulatory sequence. In some embodiments, the regulatory sequence comprises a cis-acting regulatory sequence selected from the group consisting of an enhancer, a silencer, a promoter or a response element. In some embodiments, the regulatory sequence comprises a trans-acting regulatory sequence. In some embodiments, the regulatory sequence comprises a trans-acting regulatory sequence that encodes a transcription factor. 
     In some embodiments, integration of the insert sequence does not adversely affect cell health. In some embodiments, the endonuclease, the reverse transcriptase or both are capable of site-specific integration of the insert sequence. 
     In some embodiments, the mRNA comprises a sequence encoding an additional nuclease domain or a nuclease domain that is not derived from ORF2. In some embodiments, the mRNA comprises a sequence encoding a megaTAL nuclease domain, a TALEN domain, a Cas9 domain, a zinc finger binding domain from an R2 retroelement, or a DNA binding domain that binds to repetitive sequences such as a Rep78 from AAV. In some embodiments, the endonuclease comprises a mutation that reduces activity of the endonuclease compared to the endonuclease without the mutation. In some embodiments, the endonuclease is an ORF2p endonuclease and the mutation is S228P. In some embodiments, the mRNA comprises a sequence encoding a domain that increases fidelity and/or processivity of the reverse transcriptase. In some embodiments, the reverse transcriptase is a reverse transcriptase from a retroelement other than ORF2 or reverse transcriptase that has higher fidelity and/or processivity compared to a reverse transcriptase of ORF2p. In some embodiments, the reverse transcriptase is a group II intron reverse transcriptase. In some embodiments, the group II intron reverse transcriptase is a group IIA intron reverse transcriptase, a group IIB intron reverse transcriptase, or a group IIC intron reverse transcriptase. In some embodiments, the group II intron reverse transcriptase is TGIRT-II or TGIRT-III. 
     In some embodiments, the mRNA comprises a sequence comprising an Alu element and/or a ribosome binding aptamer. In some embodiments, the mRNA comprises a sequence encoding a polypeptide comprising a DNA binding domain. In some embodiments, the 3′ UTR sequence is derived from a viral 3′ UTR or a beta-globin 3′ UTR. 
     In one aspect, provided herein is a composition comprising a recombinant mRNA or vector encoding an mRNA, wherein the mRNA comprises a human LINE-1 transposon sequence comprising a human LINE-1 transposon 5′ UTR sequence, a sequence encoding ORF1p downstream of the human LINE-1 transposon 5′ UTR sequence, an inter-ORF linker sequence downstream of the sequence encoding ORF1p,a sequence encoding ORF2p downstream of the inter-ORF linker sequence, and a 3′ UTR sequence derived from a human LINE-1 transposon downstream of the sequence encoding ORF2p; wherein the 3′ UTR sequence comprises an insert sequence, wherein the insert sequence is a reverse complement of a sequence encoding an exogenous polypeptide or a reverse complement of a sequence encoding an exogenous regulatory element. 
     In some embodiments, the insert sequence integrates into the genome of a cell when introduced into the cell. In some embodiments, the insert sequence integrates into a gene associated a condition or disease, thereby disrupting the gene or downregulating expression of the gene. In some embodiments, the insert sequence integrates into a gene, thereby upregulating expression of the gene. In some embodiments, the recombinant mRNA or vector encoding the mRNA is isolated or purified. 
     In one aspect, provided herein is a composition comprising a nucleic acid comprising a nucleotide sequence encoding (a) a long interspersed nuclear element (LINE) polypeptide, wherein the LINE polypeptide includes human ORF1p and human ORF2p; and (b) an insert sequence, wherein the insert sequence is a reverse complement of a sequence encoding an exogenous polypeptide or a reverse complement of a sequence encoding an exogenous regulatory element, wherein the composition is substantially non-immunogenic. 
     In some embodiments, the composition comprises human ORF1p and human ORF2p proteins. In some embodiments, the composition comprises a ribonucleoprotein (RNP) comprising human ORF1p and human ORF2p complexed to the nucleic acid. In some embodiments, the nucleic acid is mRNA. 
     In one aspect, provided herein is a composition comprising a cell comprising a composition described herein. In some embodiments, the cell is an immune cell. In some embodiments, the immune cell is a T cell or a B cell. In some embodiments, the immune cell is a myeloid cell. In some embodiments, the immune cell is selected from a group consisting of a monocyte, a macrophage, a dendritic cell, a dendritic precursor cell, and a macrophage precursor cell. In some embodiments, the insert sequence is a reverse complement of a sequence encoding an exogenous polypeptide and the exogenous polypeptide is a chimeric antigen receptor (CAR). 
     In one aspect, provided herein is a pharmaceutical composition comprising a composition described herein, and a pharmaceutically acceptable excipient. In some embodiments, the pharmaceutical composition is for use in gene therapy. In some embodiments, the pharmaceutical composition is for use in the manufacture of a medicament for treating a disease or condition. In some embodiments, the pharmaceutical composition is for use in treating a disease or condition. In one aspect, provided herein is a method of treating a disease in a subject, comprising administering a pharmaceutical composition described herein to a subject with a disease or condition. In some embodiments, the method increases an amount or activity of a protein or functional RNA in the subject. In some embodiments, the subject has a deficient amount or activity of a protein or functional RNA. In some embodiments, the deficient amount or activity of a protein or functional RNA is associated with or causes the disease or condition. 
     In some embodiments, the method further comprising administering an agent that inhibits human silencing hub (HUSH) complex, an agent that inhibits FAM208A, or an agent that inhibits TRIM28. In some embodiments, the agent that inhibits human silencing hub (HUSH) complex is an agent that inhibits Periphilin, TASOR and/or MPP8. In some embodiments, the agent that inhibits human silencing hub (HUSH) complex inhibits assembly of the HUSH complex. 
     In some embodiments, the agent inhibits the Fanconi anemia complex. In some embodiments, the agent inhibits FANCD2-FANC1 heterodimer monoubiquitylation. In some embodiments, the agent inhibits FANCD2-FANC1 heterodimer formation. In some embodiments the agent inhibits the Fanconi Anemia (FA) core complex. FA core complex is a component of the Fanconi anemia DNA damage repair pathway, e.g., in chemotherapy induced DNA inter-strand crosslinks. The FA core complex comprises two central dimers of the FANCB and FA-associated protein of 100 kDa (FAAP100) subunits, flanked by two copies of the RUNG finger subunit, FANCL. These two heterotrimers act as a scaffold to assemble the remaining five subunits, resulting in an extended asymmetric structure. Destabilization of the scaffold would disrupt the entire complex, resulting in a non-functional FA pathway. Examples of agents that can inhibit the FA core complex include Bortezomib and curcumin analogs EF24 and 4H-TTD. 
     In some embodiments, the sequences to be inserted may be placed under the control of tissue-specific elements, such that the entire inserted DNA is only functional in those cells in which the tissue-specific element is active. 
     In one aspect, provided herein are method and compositions for stable gene transfer to a cell by introducing to the cell a heterologous nucleic acid or gene of interest (e.g., a transgene, a regulatory sequence, for example, a sequence for an inhibitory nucleic acid, an siRNA, a miRNA), flanked by sequences that cause retrotransposition of the heterologous nucleic acid sequence into the genome of the cell. In some embodiments, the heterologous nucleic acid is termed insert for the purpose of the description in this document, where the insert is the nucleic acid sequence that will be reverse transcribed and inserted into the genome of the cell by the intended design of the constructs described herein. In some embodiments, the heterologous nucleic acid is also termed the cargo, or cargo sequence for the purpose of the description in this document. The cargo can comprise the sequence of the heterologous nucleic acid that that is inserted in the genome. In some embodiments, the cell may be a cell mammalian cell. The mammalian cell may be of epithelial, mesothelial or endothelial origin. In some embodiments, the cell may be a stem cell. In some embodiments, the cell may be a precursor cell. In some embodiments, the cell may be a cell that is terminally differentiated. In some embodiments, the cell may be a muscle cell, a cardiac cell, an epithelial cell, a hematopoietic cell, a mucous cell, an epidermal cell, a squamous cell, a cartilage cell, a bone cell, or any cell of mammalian origin. In some embodiments, the cell is of hematopoietic lineage. In some embodiments, he cell is of myeloid lineage, or a phagocytic cell, for example a monocyte, macrophage, a dendritic cell or a myeloid precursor cell. In some embodiments, the nucleic acid encoding the transgene is an mRNA. 
     In some embodiments, the retrotransposable elements may be derived from a non-LTR retrotransposon. 
     Provided herein is a method of integrating a nucleic acid sequence into a genome of a cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA into the cell, wherein the mRNA comprises an insert sequence and wherein the insert sequence is integrated into the genome of the cell. In some embodiments, the insert sequence comprises (i) an exogenous sequence, or (ii) a sequence that is a reverse complement of the exogenous sequence; a 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises a binding site for a human ORF protein. In some embodiments, the ORF protein is a human LINE 1 ORF2 protein. In some embodiments, the ORF protein is a non-human ORF protein. In some embodiments, the ORF protein is a chimeric protein, a recombinant protein or an engineered protein. 
     Provided herein is a method for integrating a nucleic acid sequence into the genome of an immune cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA, wherein the mRNA comprises, (a) an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence or (ii) a sequence that is a reverse complement of the exogenous sequence; (b) 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence, wherein the 5′ UTR sequence or the 3′ UTR sequence comprises an endonuclease binding site and a reverse transcriptase binding site, and wherein the transgene sequence is integrated into the genome of the immune cell. 
     In some embodiments, the structural elements that mediate RNA integration or transposition may be encoded in a synthetic construct and are relied upon to deliver a heterologous gene of interest to the cell. In some embodiments, the synthetic construct may comprise a nucleic acid encoding the heterologous gene of interest and the structural elements that cause integration or retrotransposition of a heterologous gene of interest into the genome. In some embodiments, the structural elements that cause integration or retrotransposition may include a 5′ L1 RNA region, and a 3′-L1 region, the latter comprising a poly A 3′ region for priming. In some embodiments, the 5′ L1 RNA region may comprise one or more stem loop regions. In some embodiments, the L1-3′ region may comprise one or more stem loop regions. In some embodiments, the 5′- and 3′ L1 regions are constructed as flanking the nucleic acid sequence encoding the heterologous gene of interest (the transgene). In some embodiments, the structural elements may include a region from an L1 or an Alu RNA comprising the hairpin loop structure that includes the A-Box and the B-Box elements that are ribosomal binding sites In some embodiments, the synthetic nucleic acid may comprise a L1-Ta promoter. 
     There may be two types of LINE RNA recognition by ORF2p—the stringent and the relaxed. In the stringent type RT recognizes its own 3′UTR tail, and in the relaxed type RT does not require any specific recognition except for the poly-A tail. Division into the stringent and the relaxed type came from the observation that some LINE/SINE pairs share the same 3′-end. For the stringent type, the experimental studies showed that a 3′UTR stem-loop promotes retrotransposition. The 5′-UTR of the LINE retrotransposition sequences have been shown to contain three conserved stem loop regions. 
     In some embodiments, the transgene, or transcript of interest may be flanked by transposable elements from a L1 or an Alu sequence at the 5′ and the 3′ end. In some embodiments, the 5′ region of a retrotransposon comprises an Alu sequence. In some embodiments, the 3′ region of a retrotransposon comprises an Alu sequence. In some embodiments, the 5′ region of a retrotransposon comprises an L1 sequence. In some embodiments, the 3′ region of a retrotransposon comprises an L1 sequence. In some embodiments, the transgene or transcript of interest is flanked by an SVA transposon sequence. 
     In some embodiments, the transcript of interest may comprise an L1 or an Alu sequence, encoding the binding regions for ORF2p and the 3′-poly A priming regions. In some embodiments, the heterologous nucleic acid encoding the transgene of interest may be flanked by an L1 or an Alu sequence, encoding the binding regions for ORF1p and the 3′-poly A priming regions. The 3′-region may comprise one or more stem loop structures. In some embodiments, the transcript of interest is structured for cis integration or retrotransposition. In some embodiments, the transcript of interest is structured for trans integration or retrotransposition. 
     In some embodiments, the retrotransposon is a human retrotransposon. The sequence of a human retrotransposon can comprise a sequence encoding an endonuclease and/or a reverse transcriptase. The sequence of a human retrotransposon can encode for two proteins that are translated from a single RNA containing two non-overlapping ORFs. In some embodiments, the two ORFs are ORF1 and ORF2. 
     Accordingly, provided herein is a method for stably integrating a heterologous nucleic acid encoding a transgene into the genome of a cell, such as a myeloid cell, the method comprising introducing to the cell a nucleic acid encoding: the transgene; one or more 5′ nucleic acid sequences flanking the region encoding the transgene, comprising a 5′ region of a retrotransposon; and one or more 3′ nucleic acid sequence flanking the region encoding the transgene, comprising a 3′ region of a retrotransposon, wherein the 3′ region of the retrotransposon comprises a genomic DNA priming sequence and a LINE transposase binding sequence, having the respective endonuclease and reverse transcriptase (RT) activity. 
     Provided herein is a method for integrating a nucleic acid sequence into the genome of a cell, the method comprising introducing a recombinant mRNA or a vector encoding an mRNA, wherein the mRNA comprises an insert sequence, wherein the insert sequence comprises (i) an exogenous sequence or (ii) a sequence that is a reverse complement of the exogenous sequence; (b) a 5′ UTR sequence, a sequence of a human retrotransposon downstream of the 5′ UTR sequence, and a 3′ UTR sequence downstream of the sequence of a human retrotransposon; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises an endonuclease binding site and a reverse transcriptase binding site, and wherein the sequence of a human retrotransposon encodes for two proteins that are translated from a single RNA containing two ORFs, and wherein the insert sequence is integrated into the genome of the cell. 
     In some embodiments, the method comprising using a single nucleic acid molecule for delivering and integrating the insert sequence into the genome of a cell. The single nucleic acid molecule may be a plasmid vector. The single nucleic acid may be DNA or an RNA molecule. The single nucleic acid may be an mRNA. 
     In some embodiments, the method comprises introducing into a cell one or more polynucleotides comprising the human retrotransposon and a heterologous nucleic acid sequence. In some embodiments, the one or more polynucleotides comprises (i) a first nucleic acid molecule encoding an ORF1p; (ii) a second nucleic acid molecule encoding an ORF2p and a sequence encoding a cargo. In some embodiments, the first nucleic acid and the second nucleic acid are mRNA. In some embodiments, the first nucleic acid and the second nucleic acid are DNA, e.g., encoded in separate plasmid vectors. 
     Provided herein is a self-integrating polynucleotide that comprises a sequence which is inserted into the genome of a cell, and insert is stably integrated into the genome by the self-integrating naked polynucleotide. In some embodiments, the polynucleotide is an RNA. In some embodiments, the polynucleotide is an mRNA. In some embodiments, the polynucleotide is an mRNA that has modifications. In some embodiments, the modifications ensure protection against RNases in the intracellular milieu. In some embodiments, the modifications include substituted modified nucleotides, e.g., 5-methylcytidine, pseudouridine or 2-thiouridine. 
     In some embodiments, a single polynucleotide is used for delivery and genomic integration of the insert (or cargo) nucleic acid. In some embodiments, the single polynucleotide is bicistronic. In some embodiments, the single polynucleotide is tricistronic. In some embodiments, the single polynucleotide is multi-cistronic. In some embodiments, a two or more polynucleotide molecules are used for delivery and genomic integration of the insert (or cargo) nucleic acid. 
     In some embodiments, a retrotransposable genetic element may be generated, the retrotransposable genetic element comprising (i) a heterologous nucleic acid encoding a transgene or a non-coding sequence to be inserted into the genome of a cell (the insert); (ii) a nucleic sequence encoding one or more retrotransposon ORF-encoding sequences; (iii) one or more UTR regions of the ORF-coding sequences, such that the heterologous nucleic acid encoding a transgene or a non-coding sequence to be inserted is comprised within the UTR sequences; wherein the 3′ region of the retrotransposon ORF-encoding sequences comprises a genomic DNA priming sequence. 
     In some embodiments, the retrotransposable genetic element may be introduced into a cell for stably integrating the transgene into the genomic DNA. In some embodiments, the retrotransposable genetic element comprises (a) a retrotransposon protein coding sequence, and a 3′ UTR; and (b) a sequence comprising a heterologous nucleic acid that is to be inserted (e.g., integrated) within the genome of a cell. The retrotransposon protein coding sequence, and the 3′ UTR may be a complete and sufficient unit for delivering the heterologous nucleic acid sequence within the genome of the cell, and comprise the retrotransposable elements, such as an endonuclease, a reverse transcriptase, a sequence in the 3′ UTR for binding to and priming the genomic DNA at the region cleaved by the endonuclease to start reverse transcribing and incorporating the heterologous nucleic acid. 
     In some embodiments, the coding sequence of the insert is in forward orientation with respect to the coding sequence of the one or more ORFs. In some embodiments, the coding sequence of the insert is in reverse orientation with respect to the coding sequence of the one or more ORFs. The coding sequence of the insert and the coding sequence of the one or more ORFs may comprise distinct regulatory elements, including 5′ UTR, 3′ UTR, promoter, enhancer, etc. In some embodiments, the 3′ UTR or the 5′-UTR of the insert may comprise the coding sequence of the one or more ORFs, and likewise, the coding sequence of the insert may be situated within in the 3′ UTR of the coding sequence of the one or more ORFs. 
     In some embodiments, a retrotransposable genetic element may be generated, the retrotransposable genetic element comprising: (a) an insert sequence, comprising (i) an exogenous sequence, a sequence that is a reverse complement of the exogenous sequence; a 5′ UTR sequence and a 3′ UTR sequence downstream of the 5′ UTR sequence; wherein the 5′ UTR sequence or the 3′ UTR sequence comprises a binding site for a human ORF protein. 
     In some embodiments, the retrotransposon may comprise a SINE or LINE element. In some embodiments, the retrotransposon comprises a SINE or LINE stem loop structure, such as an Alu element. 
     In some embodiments, the retrotransposon is a LINE-1 (L1) retrotransposon. In some embodiments, the retrotransposon is human LINE-1. Human LINE-1 sequences are abundant in the human genome. There are approximately 13,224 total human L1s, of which 480 are active, which make up about 3.6%. Therefore, human L1 proteins are well tolerated and non-immunogenic in humans. Moreover, a tight regulation of random transposition in human ensures that random transposase activity will not be triggered by introduction of the L1 system as described herein. In addition, the retrotransposable constructs designed herein may comprise targeted and specific incorporation of the insert sequence. In some embodiments, the retrotransposable genetic element may comprise designs intended to overcome the silencing machinery actively prevalent in human cells, while being careful that random integration resulting in genomic instability is not initiated. 
     Accordingly, the retrotransposable constructs may comprise a sequence encoding a human LINE-1 ORF1 protein; and a human LINE-1 ORF2 protein. In some embodiments, the construct comprises a nucleic acid sequence encoding an ORF1p protein with at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to MGKKQNRKTGNSKTQSASPPPKERSSSPATEQSWMENDFDELREEGFRRSNYSELREDIQTKGK EVENFEKNLEECITRITNTEKCLKELMELKTKARELREECRSLRSRCDQLEERVSAMEDEMNEM KREGKFREKRIKRNEQSLQEIWDYVKRPNLRLIGVPESDVENGTKLENTLQDIIQENFPNLARQA NVQIQEIQRTPQRYSSRRATPRHIIVRFTKVEMKEKMLRAAREKGRVTLKGKPIRLTVDLSAETL QARREWGPIFNILKEKNFQPRISYPAKLSFISEGEIKYFIDKQMLRDFVTTRPALKELLKEALNME RNNRYQPLQNHAKM (SEQ ID NO: 57). In some embodiments, the construct comprises a nucleic acid sequence with at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 58)  
               
               
                   
                 atgggcaagaagcaaaatcgcaagacggggaattc 
               
               
                   
                   
               
               
                   
                 caagacacaatccgctagcccaccacctaaagagc 
               
               
                   
                   
               
               
                   
                 gttctagctcccctgctactgagcagtcctggatg 
               
               
                   
                   
               
               
                   
                 gaaaacgacttcgatgaactccgggaagagggatt 
               
               
                   
                   
               
               
                   
                 taggcgatccaactattcagaactccgcgaagata 
               
               
                   
                   
               
               
                   
                 tccagacaaaggggaaggaagtcgagaatttcgag 
               
               
                   
                   
               
               
                   
                 aagaacctcgaggagtgcatcacccgtatcacaaa 
               
               
                   
                   
               
               
                   
                 cactgagaaatgtctcaaagaactcatggaactta 
               
               
                   
                   
               
               
                   
                 agacaaaagccagggagcttcgagaggagtgtcgg 
               
               
                   
                   
               
               
                   
                 agtctgagatccaggtgtgaccagctcgaggagcg 
               
               
                   
                   
               
               
                   
                 cgtgagcgcgatggaagacgagatgaacgagatga 
               
               
                   
                   
               
               
                   
                 aaagagagggcaaattcagggagaagcgcattaag 
               
               
                   
                   
               
               
                   
                 aggaacgaacagagtctgcaggagatttgggatta 
               
               
                   
                   
               
               
                   
                 cgtcaagaggcctaacctgcggttgatcggcgtcc 
               
               
                   
                   
               
               
                   
                 ccgagagcgacgtagaaaacgggactaaactggag 
               
               
                   
                   
               
               
                   
                 aatacacttcaagacatcattcaagaaaattttcc 
               
               
                   
                   
               
               
                   
                 aaacctggctcggcaagctaatgtgcaaatccaag 
               
               
                   
                   
               
               
                   
                 agatccaacgcacaccccagcggtatagctctcgg 
               
               
                   
                   
               
               
                   
                 cgtgccacccctaggcatattatcgtgcgctttac 
               
               
                   
                   
               
               
                   
                 taaggtggagatgaaagagaagatgctgcgagccg 
               
               
                   
                   
               
               
                   
                 ctcgggaaaagggaagggtgactttgaagggcaaa 
               
               
                   
                   
               
               
                   
                 cctattcggctgacggttgaccttagcgccgagac 
               
               
                   
                   
               
               
                   
                 actccaggcacgccgggaatggggccccatcttta 
               
               
                   
                   
               
               
                   
                 atatcctgaaggagaagaacttccagccacgaatc 
               
               
                   
                   
               
               
                   
                 tcttaccctgcaaagttgagttttatctccgaggg 
               
               
                   
                   
               
               
                   
                 tgagattaagtatttcatcgataaacagatgctgc 
               
               
                   
                   
               
               
                   
                 gagacttcgtgacaactcgcccagctctcaaggaa 
               
               
                   
                   
               
               
                   
                 ctgctcaaagaggctcttaatatggagcgcaataa 
               
               
                   
                   
               
               
                   
                 tagatatcaacccttgcagaaccacgcaaagatg 
               
               
                   
                   
               
               
                   
                 tga. 
               
            
           
         
       
     
     In some embodiments, the construct comprises a nucleic acid sequence encoding an ORF2p protein with at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to MTGSNSHITILTLNINGLNSAIKRHRLASWIKSQDPSVCCIQETHLTCRDTHRLKIKGWRKIYQAN GKQKKAGVAILVSDKTDFKPTKIKRDKEGHYIMVKGSIQQEELTILNIYAPNTGAPRFIKQVLSDL QRDLDSHTLIMGDFNTPLSTLDRSTRQKVNKDTQELNSALHQADLIDIYRTLHPKSTEYTFFSAP HHTYSKIDHIVGSKALLSKCKRTEIITNYLSDHSAIKLELRIKNLTQSRSTTWKLNNLLLNDYWV HNEMKAEIKMFFETNENKDTTYQNLWDAFKAVCRGKFIALNAYKRKQERSKIDTLTSQLKELE KQEQTHSKASRRQEITKIRAELKEIETQKTLQKINESRSWFFERINKIDRPLARLIKKKREKNQIDTI KNDKGDITTDPTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLPRLNQEEVESLNRPITGSEIV AIINSLPTKKSPGPDGFTAEFYQRYMEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTKKE NFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNH MIISIDAEKAFDKIQQPFMLKTLNKLGIDGTYFKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGC PLSPLLFNIVLEVLARAIRQEKEIKGIQLGKEEVKLSLFADDMIVYLENPIVSAQNLLKLISNFSKV SGYKINVQKSQAFLYTNNRQTESQIMGELPFVIASKRIKYLGIQLTRDVKDLFKENYKPLLKEIKE DTNKWKNIPCSWVGRINIVKMAILPKVIYRFNAIPIKLPMTFFTELEKTTLKFIWNQKRARIAKSIL SQKNKAGGITLPDFKLYYKATVTKTAWYWYQNRDIDQWNRTEPSEIMPHIYNYLIFDKPEKNK QWGKDSLFNKWCWENWLAICRKLKLDPFLTPYTKINSRWIKDLNVKPKTIKTLEENLGITIQDIG VGKDFMSKTPKAMATKDKIDKWDLIKLKSFCTAKETTIRVNRQPTTWEKIFATYSSDKGLISRIY NELKQIYKKKTNNPIKKWAKDMNRHFSKEDIYAAKKHMKKCSSSLAIREMQIKTTMRYHLTPV RMAIIKKSGNNRCWRGCGEIGTLLHCWWDCKLVQPLWKSVWRFLRDLELEIPFDPAIPLLGIYP NEYKSCCYKDTCTRMFIAALFTIAKTWNQPKCPTMIDWIKKMWHIYTMEYYAAIKNDEFISFVG TWMKLETIILSKLSQEQKTKHRIFSLIGGN (SEQ ID NO: 59). In some embodiments, the construct comprises a nucleic acid sequence with at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 60) 
               
               
                   
                 atgaccggctctaactcacatatcaccatccttac 
               
               
                   
                   
               
               
                   
                 acttaacattaacggcctcaactcagctatcaagc 
               
               
                   
                   
               
               
                   
                 gccatcggctggccagctggatcaaatcacaggat 
               
               
                   
                   
               
               
                   
                 ccaagcgtttgttgcatccaagagacccacctgac 
               
               
                   
                   
               
               
                   
                 ctgtagagatactcaccgcctcaagatcaagggat 
               
               
                   
                   
               
               
                   
                 ggcgaaagatttatcaggcgaacggtaagcagaag 
               
               
                   
                   
               
               
                   
                 aaagccggagtcgcaattctggtctcagacaagac 
               
               
                   
                   
               
               
                   
                 ggatttcaagcccaccaaaattaagcgtgataagg 
               
               
                   
                   
               
               
                   
                 aaggtcactatattatggtgaaaggcagcatacag 
               
               
                   
                   
               
               
                   
                 caggaagaacttaccatattgaacatctacgcgcc 
               
               
                   
                   
               
               
                   
                 aaacaccggcgcacctcgctttatcaaacaggtcc 
               
               
                   
                   
               
               
                   
                 tgtccgatctgcagcgagatctggattctcatacg 
               
               
                   
                   
               
               
                   
                 ttgattatgggtgatttcaatacaccattgagcac 
               
               
                   
                   
               
               
                   
                 cctggatcgcagcaccaggcaaaaggtaaataaag 
               
               
                   
                   
               
               
                   
                 acacgcaagagctcaatagcgcactgcatcaggca 
               
               
                   
                   
               
               
                   
                 gatctcattgatatttatcgcactcttcatcctaa 
               
               
                   
                   
               
               
                   
                 gagtaccgagtacacattcttcagcgccccacatc 
               
               
                   
                   
               
               
                   
                 atacatactcaaagatcgatcatatcgtcggctca 
               
               
                   
                   
               
               
                   
                 aaggctctgctgtcaaagtgcaagcgcacagagat 
               
               
                   
                   
               
               
                   
                 aattacaaattacctgtcagatcatagcgcgatca 
               
               
                   
                   
               
               
                   
                 agctcgagctgagaatcaagaacctgacccagagc 
               
               
                   
                   
               
               
                   
                 cggagtaccacttggaagcttaataacctgctgct 
               
               
                   
                   
               
               
                   
                 caacgattattgggtccacaatgagatgaaggcag 
               
               
                   
                   
               
               
                   
                 agattaaaatgttcttcgaaacaaatgagaataag 
               
               
                   
                   
               
               
                   
                 gatactacctatcaaaacctttgggatgcctttaa 
               
               
                   
                   
               
               
                   
                 ggccgtctgcagaggcaagttcatcgccctcaacg 
               
               
                   
                   
               
               
                   
                 cctataaaagaaaacaagagagatctaagatcgat 
               
               
                   
                   
               
               
                   
                 actctcacctctcagctgaaggagttggagaaaca 
               
               
                   
                   
               
               
                   
                 ggaacagacccactccaaggcgtcaagacggcagg 
               
               
                   
                   
               
               
                   
                 agatcacaaagattcgcgccgagttgaaagagatc 
               
               
                   
                   
               
               
                   
                 gaaacccaaaagactcttcagaaaattaacgagtc 
               
               
                   
                   
               
               
                   
                 tcgtagttggttcttcgagcggattaataagatag 
               
               
                   
                   
               
               
                   
                 acagacctctggcacgactgattaagaagaagcgc 
               
               
                   
                   
               
               
                   
                 gaaaagaaccagattgataccatcaagaacgacaa 
               
               
                   
                   
               
               
                   
                 gggcgacatcactactgacccgaccgagatccaga 
               
               
                   
                   
               
               
                   
                 ccactattcgggagtattataagcatttgtatgct 
               
               
                   
                   
               
               
                   
                 aacaagcttgagaacctggaagagatggacacttt 
               
               
                   
                   
               
               
                   
                 tctggatacctatactctgccacggcttaatcaag 
               
               
                   
                   
               
               
                   
                 aggaagtcgagtccctcaaccgcccaattacagga 
               
               
                   
                   
               
               
                   
                 agcgagattgtggccataattaactccctgccgac 
               
               
                   
                   
               
               
                   
                 aaagaaatctcctggtccggacgggtttacagctg 
               
               
                   
                   
               
               
                   
                 agttttatcaacggtatatggaagagcttgtaccg 
               
               
                   
                   
               
               
                   
                 tttctgctcaagctctttcagtctatagaaaagga 
               
               
                   
                   
               
               
                   
                 aggcatcttgcccaattccttctacgaagcttcta 
               
               
                   
                   
               
               
                   
                 taatacttattcccaaaccaggacgcgataccaca 
               
               
                   
                   
               
               
                   
                 aagaaggaaaacttccggcccattagtctcatgaa 
               
               
                   
                   
               
               
                   
                 tatcgacgctaaaatattgaacaagattctcgcca 
               
               
                   
                   
               
               
                   
                 acagaatccaacaacatattaagaaattgatacat 
               
               
                   
                   
               
               
                   
                 cacgaccaggtggggtttatacctggcatgcaggg 
               
               
                   
                   
               
               
                   
                 ctggtttaacatccggaagagtattaacgtcattc 
               
               
                   
                   
               
               
                   
                 aacacattaatagagctaaggataagaatcatatg 
               
               
                   
                   
               
               
                   
                 atcatctctatagacgcggaaaaggcattcgataa 
               
               
                   
                   
               
               
                   
                 gattcagcagccatttatgctcaagactctgaaca 
               
               
                   
                   
               
               
                   
                 aactcggcatcgacggaacatattttaagattatt 
               
               
                   
                   
               
               
                   
                 cgcgcaatttacgataagccgactgctaacattat 
               
               
                   
                   
               
               
                   
                 ccttaacggccaaaagctcgaggcctttccgctca 
               
               
                   
                   
               
               
                   
                 agactggaacccgccaaggctgtcccctctccccg 
               
               
                   
                   
               
               
                   
                 cttttgtttaatattgtactcgaggtgctggctag 
               
               
                   
                   
               
               
                   
                 ggctattcgtcaagagaaagagattaaagggatac 
               
               
                   
                   
               
               
                   
                 agctcgggaaggaagaggtcaagctttccttgttc 
               
               
                   
                   
               
               
                   
                 gccgatgatatgattgtgtacctggagaatcctat 
               
               
                   
                   
               
               
                   
                 tgtgtctgctcagaaccttcttaaacttatttcta 
               
               
                   
                   
               
               
                   
                 actttagcaaggtcagcggctataagattaacgtc 
               
               
                   
                   
               
               
                   
                 cagaaatctcaggcctttctgtacacaaataatcg 
               
               
                   
                   
               
               
                   
                 acagaccgaatcccagataatgggtgagcttccgt 
               
               
                   
                   
               
               
                   
                 ttgtcatagccagcaaaaggataaagtatctcgga 
               
               
                   
                   
               
               
                   
                 atccagctgacacgagacgttaaagatttgtttaa 
               
               
                   
                   
               
               
                   
                 ggaaaattacaagcctctcctgaaagagattaagg 
               
               
                   
                   
               
               
                   
                 aagatactaataagtggaagaatatcccctgttca 
               
               
                   
                   
               
               
                   
                 tgggttggcagaatcaacatagtgaagatggcaat 
               
               
                   
                   
               
               
                   
                 acttcctaaagtgatatatcgctttaacgccatcc 
               
               
                   
                   
               
               
                   
                 caattaaactgcctatgaccttctttacggagctc 
               
               
                   
                   
               
               
                   
                 gagaaaacaacccttaaatttatatggaatcaaaa 
               
               
                   
                   
               
               
                   
                 gagagcaagaatagcgaagtccatcttgagccaga 
               
               
                   
                   
               
               
                   
                 agaataaggccggtgggattactttgcctgatttt 
               
               
                   
                   
               
               
                   
                 aagttgtattataaagccacagtaactaagacagc 
               
               
                   
                   
               
               
                   
                 ctggtattggtatcagaatagagacatcgaccagt 
               
               
                   
                   
               
               
                   
                 ggaatcggaccgaaccatcagagataatgccccac 
               
               
                   
                   
               
               
                   
                 atctataattaccttatattcgataagccagaaaa 
               
               
                   
                   
               
               
                   
                 gaataaacagtggggcaaagacagcctcttcaaca 
               
               
                   
                   
               
               
                   
                 agtggtgttgggagaattggctggccatatgccgg 
               
               
                   
                   
               
               
                   
                 aaactcaagctcgacccctttcttacaccctacac 
               
               
                   
                   
               
               
                   
                 taaaatcaacagtaggtggatcaaggacttgaatg 
               
               
                   
                   
               
               
                   
                 tcaagccaaagactataaagacactggaagagaat 
               
               
                   
                   
               
               
                   
                 cttgggatcacaatacaagatataggcgtcggcaa 
               
               
                   
                   
               
               
                   
                 agattttatgtcaaagacgcccaaggccatggcca 
               
               
                   
                   
               
               
                   
                 ctaaggataagattgataagtgggaccttattaag 
               
               
                   
                   
               
               
                   
                 ctcaaaagcttctgtactgccaaggagaccacgat 
               
               
                   
                   
               
               
                   
                 cagagttaataggcagcccactacatgggaaaaga 
               
               
                   
                   
               
               
                   
                 ttttcgccacttattcatcagataaggggttgata 
               
               
                   
                   
               
               
                   
                 agcagaatatataacgagctgaagcagatctacaa 
               
               
                   
                   
               
               
                   
                 gaagaaaacgaataatcccatcaagaagtgggcaa 
               
               
                   
                   
               
               
                   
                 aagatatgaacaggcattttagcaaagaggatatc 
               
               
                   
                   
               
               
                   
                 tacgccgcgaagaagcatatgaagaagtgtagttc 
               
               
                   
                   
               
               
                   
                 aagcttggccattcgtgagatgcagattaagacga 
               
               
                   
                   
               
               
                   
                 ccatgcgataccaccttaccccagtgaggatggca 
               
               
                   
                   
               
               
                   
                 attatcaagaaatctggcaataatagatgttggcg 
               
               
                   
                   
               
               
                   
                 gggctgtggcgagattggcaccctgctccattgct 
               
               
                   
                   
               
               
                   
                 ggtgggattgcaagctggtgcagccgctttggaaa 
               
               
                   
                   
               
               
                   
                 tcagtctggcgctttctgagggacctcgagcttga 
               
               
                   
                   
               
               
                   
                 gattcccttcgatcccgcaattcccttgctcggaa 
               
               
                   
                   
               
               
                   
                 tctatcctaacgaatacaagagctgttgttacaag 
               
               
                   
                   
               
               
                   
                 gatacgtgtacccggatgttcatcgcggccttgtt 
               
               
                   
                   
               
               
                   
                 tacgatagctaagacgtggaatcagcctaagtgcc 
               
               
                   
                   
               
               
                   
                 ccacaatgatcgattggatcaagaaaatgtggcat 
               
               
                   
                   
               
               
                   
                 atttataccatggagtattacgcagcaattaagaa 
               
               
                   
                   
               
               
                   
                 tgacgaatttatttccttcgttgggacctggatga 
               
               
                   
                   
               
               
                   
                 agctggagactattattctgagcaagctgtctcag 
               
               
                   
                   
               
               
                   
                 gagcaaaagacaaagcatagaatcttctctctcat 
               
               
                   
                   
               
               
                   
                 tggtggtaactaa. 
               
            
           
         
       
     
     In some embodiments, the construct comprises a nucleic acid sequence encoding an ORF2p protein with at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to MVIGTYISIITLNVNGLNAPTKRHRLAEWIQKQDPYICCLQETHFRPRDTYRLKVRGWKKIFHAN GNQKKAGVAILISDKIDFKIKNVTRDKEGHYIMIQGSIQEEDITIINIYAPNIGAPQYIRQLLTAIKE EIDSNTIIVGDFNTSLTPMDRSSKMKINKETEALNDTIDQIDLIDIYRTFHPKTADYTFFSSAHGTFS RIDHILGHKSSLSKFKKIEIISSIFSDHNAMRLEMNHREKNVKKTNTWRLNNTLLNNQEITEEIKQ EIKKYLETNDNENTTTQNLWDAAKAVLRGKFIAIQAYLKKQEKSQVNNLTLHLKKLEKEEQTK PKVSRRKEIIKIRAEINEIETKKTIAKINKTKSWFFEKINKIDKPLARLIKKKRERTQINKIRNEKGE VTTDTAEIQNILRDYYKQLYANKMDNLEEMDKFLERYNLPRLNQEETENINRPITSNEIETVIKNL PTNKSPGPDGFTGEFYQTFREELTPILLKLFQKIAEEGTLPNSFYEATITLIPKPDKDTTKKENYRPI SLMNIDAKILNKILANRIQQHIKRIIHHDQVGFIPGMQGFFNIRKSINVIHHINKLKKKNHMIISIDA EKAFDKIQHPFMIKTLQKVGIEGTYLNIIKAIYDKPTANIILNGEKLKAFPLRSGTRQGCPLSPLLF NIVLEVLATAIREEKEIKGIQIGKEEVKLSLFADDMILYIENPKTATRKLLELINEYGKVAGYKINA QKSLAFLYTNDEKSEREIMETLPFTIATKRIKYLGINLPKETKDLYAENYKTLMKEIKDDTNRWR DIPCSWIGRINIVKMSILPKAIYRFNAIPIKLPMAFFTELEQIILKFVWRHKRPRIAKAVLRQKNGA GGIRLPDFRLYYKATVIKTIWYWHKNRNIDQWNKIESPEINPRTYGQLIYDKGGKDIQWRKDSLF NKWCWENWTATCKRMKLEYSLTPYTKINSKWIRDLNIRLDTIKLLEENIGRTLFDINHSKIFFDPP PRVMEIKTKINKWDLMKLQSFCTAKETINKTKRQPSEWEKIFANESTDKGLISKIYKQLIQLNIKE TNTPIQKWAEDLNRHFSKEDIQTATKHMKRCSTSLIIREMQIKTTMRYHLTPVRMGIIRKSTNNK CWRGCGEKGTLLHCWWECKLIQPLWRTIWRFLKKLKIELPYDPAIPLLGIYPEKTVIQKDTCTR MFIAALFTIARSWKQPKCPSTDEWIKKMWYIYTMEYYSAIKRNEIGSFLETWMDLETVIQSEVSQ KEKNKYRILTHICGTWKNGTDEPVCRTEIETQM (SEQ ID NO: 61). In some embodiments, the construct comprises a nucleic acid sequence with at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 62) 
               
               
                   
                 atggtcataggaacatacatatcgataattacctt 
               
               
                   
                   
               
               
                   
                 aaacgtgaatggattaaatgccccaaccaaaagac 
               
               
                   
                   
               
               
                   
                 atagactggctgaatggatacaaaaacaagaccca 
               
               
                   
                   
               
               
                   
                 tatatatgctgtctacaagagacccacttcagacc 
               
               
                   
                   
               
               
                   
                 tagggacacatacagactgaaagtgaggggatgga 
               
               
                   
                   
               
               
                   
                 aaaagatattccatgcaaatggaaatcaaaagaaa 
               
               
                   
                   
               
               
                   
                 gctggagtagctatactcatatcagataaaataga 
               
               
                   
                   
               
               
                   
                 ctttaaaataaagaatgttacaagagacaaggaag 
               
               
                   
                   
               
               
                   
                 gacactacataatgatccagggatcaatccaagaa 
               
               
                   
                   
               
               
                   
                 gaagatataacaattataaatatatatgcacccaa 
               
               
                   
                   
               
               
                   
                 cataggagcacctcaatacataaggcaactgctaa 
               
               
                   
                   
               
               
                   
                 cagctataaaagaggaaatcgacagtaacacaata 
               
               
                   
                   
               
               
                   
                 atagtgggggactttaacacctcacttacaccaat 
               
               
                   
                   
               
               
                   
                 ggacagatcatccaaaatgaaaataaataaggaaa 
               
               
                   
                   
               
               
                   
                 cagaagctttaaatgacacaatagaccagatagat 
               
               
                   
                   
               
               
                   
                 ttaattgatatatataggacattccatccaaaaac 
               
               
                   
                   
               
               
                   
                 agcagattacacgttcttctcaagtgcgcacggaa 
               
               
                   
                   
               
               
                   
                 cattctccaggatagatcacatcttgggtcacaaa 
               
               
                   
                   
               
               
                   
                 tcaagcctcagtaaatttaagaaaattgaaatcat 
               
               
                   
                   
               
               
                   
                 atcaagcatcttttctgaccacaacgctatgagat 
               
               
                   
                   
               
               
                   
                 tagaaatgaatcacagggaaaaaaacgtaaaaaag 
               
               
                   
                   
               
               
                   
                 acaaacacatggaggctaaacaatacgttactaaa 
               
               
                   
                   
               
               
                   
                 taaccaagagatcactgaagaaatcaaacaggaaa 
               
               
                   
                   
               
               
                   
                 taaaaaaatacctagagacaaatgacaatgaaaac 
               
               
                   
                   
               
               
                   
                 acgacgacccaaaacctatgggatgcagcaaaagc 
               
               
                   
                   
               
               
                   
                 ggttctaagagggaagtttatagctatacaagcct 
               
               
                   
                   
               
               
                   
                 acctaaagaaacaagaaaaatctcaagtaaacaat 
               
               
                   
                   
               
               
                   
                 ctaaccttacacctaaagaaactagagaaagaaga 
               
               
                   
                   
               
               
                   
                 acaaacaaaacccaaagttagcagaaggaaagaaa 
               
               
                   
                   
               
               
                   
                 tcataaagatcagagcagaaataaatgaaatagaa 
               
               
                   
                   
               
               
                   
                 acaaagaaaacaatagcaaagatcaataaaactaa 
               
               
                   
                   
               
               
                   
                 aagttggttctttgagaagataaacaaaattgata 
               
               
                   
                   
               
               
                   
                 agccattagccagactcatcaagaaaaagagggag 
               
               
                   
                   
               
               
                   
                 aggactcaaatcaataaaatcagaaatgaaaaagg 
               
               
                   
                   
               
               
                   
                 agaagttacaacagacaccgcagaaatacaaaaca 
               
               
                   
                   
               
               
                   
                 tcctaagagactactacaagcaactttatgccaat 
               
               
                   
                   
               
               
                   
                 aaaatggacaacctggaagaaatggacaaattctt 
               
               
                   
                   
               
               
                   
                 agaaaggtataaccttccaagactgaaccaggaag 
               
               
                   
                   
               
               
                   
                 aaacagaaaatatcaacagaccaatcacaagtaat 
               
               
                   
                   
               
               
                   
                 gaaattgaaactgtgattaaaaatcttccaacaaa 
               
               
                   
                   
               
               
                   
                 caaaagtccaggaccagatggcttcacaggtgaat 
               
               
                   
                   
               
               
                   
                 tctatcaaacatttagagaagagctaacacccatc 
               
               
                   
                   
               
               
                   
                 cttctcaaactcttccaaaaaattgcagaagaagg 
               
               
                   
                   
               
               
                   
                 aacactcccaaactcattctatgaggccaccatca 
               
               
                   
                   
               
               
                   
                 ccctgataccaaaaccagacaaagacactacaaaa 
               
               
                   
                   
               
               
                   
                 aaagaaaattacagaccaatatcactgatgaatat 
               
               
                   
                   
               
               
                   
                 agatgcaaaaatcctcaacaaaatactagcaaaca 
               
               
                   
                   
               
               
                   
                 gaatccaacaacacattaaaaggatcatacaccac 
               
               
                   
                   
               
               
                   
                 gatcaagtgggatttatcccagggatgcaaggatt 
               
               
                   
                   
               
               
                   
                 cttcaatatacgcaaatcaatcaatgtgatacacc 
               
               
                   
                   
               
               
                   
                 atattaacaaattgaagaagaaaaaccatatgatc 
               
               
                   
                   
               
               
                   
                 atctcaatagatgcagaaaaagcttttgacaaaat 
               
               
                   
                   
               
               
                   
                 tcaacacccatttatgataaaaactctccagaaag 
               
               
                   
                   
               
               
                   
                 tgggcatagagggaacctacctcaacataataaag 
               
               
                   
                   
               
               
                   
                 gccatatatgacaaacccacagcaaacatcattct 
               
               
                   
                   
               
               
                   
                 caatggtgaaaaactgaaagcatttcctctaagat 
               
               
                   
                   
               
               
                   
                 caggaacgagacaaggatgtccactctcaccacta 
               
               
                   
                   
               
               
                   
                 ttattcaacatagttctggaagtcctagccacggc 
               
               
                   
                   
               
               
                   
                 aatcagagaagaaaaagaaataaaaggaatacaaa 
               
               
                   
                   
               
               
                   
                 ttggaaaagaagaagtaaaactgtcactgtttgcg 
               
               
                   
                   
               
               
                   
                 gatgacatgatactatacatagagaatcctaaaac 
               
               
                   
                   
               
               
                   
                 tgccaccagaaaactgctagagctaattaatgaat 
               
               
                   
                   
               
               
                   
                 atggtaaagttgcaggttacaaaattaatgcacag 
               
               
                   
                   
               
               
                   
                 aaatctcttgcattcctatacactaatgatgaaaa 
               
               
                   
                   
               
               
                   
                 atctgaaagagaaattatggaaacactcccattta 
               
               
                   
                   
               
               
                   
                 ccattgcaacaaaaagaataaaatacctaggaata 
               
               
                   
                   
               
               
                   
                 aacctacctaaggagacaaaagacctgtatgcaga 
               
               
                   
                   
               
               
                   
                 aaactataagacactgatgaaagaaattaaagatg 
               
               
                   
                   
               
               
                   
                 ataccaacagatggagagatataccatgttcttgg 
               
               
                   
                   
               
               
                   
                 attggaagaatcaacattgtgaaaatgagtatact 
               
               
                   
                   
               
               
                   
                 acccaaagcaatctacagattcaatgcaatcccta 
               
               
                   
                   
               
               
                   
                 tcaaattaccaatggcattttttacggagctagaa 
               
               
                   
                   
               
               
                   
                 caaatcatcttaaaatttgtatggagacacaaaag 
               
               
                   
                   
               
               
                   
                 accccgaatagccaaagcagtcttgaggcaaaaaa 
               
               
                   
                   
               
               
                   
                 atggagctggaggaatcagactccctgacttcaga 
               
               
                   
                   
               
               
                   
                 ctatactacaaagctacagtaatcaagacaatatg 
               
               
                   
                   
               
               
                   
                 gtactggcacaaaaacagaaacatagatcaatgga 
               
               
                   
                   
               
               
                   
                 acaagatagaaagcccagagattaacccacgcacc 
               
               
                   
                   
               
               
                   
                 tatggtcaactaatctatgacaaaggaggcaaaga 
               
               
                   
                   
               
               
                   
                 tatacaatggagaaaagacagtctcttcaataagt 
               
               
                   
                   
               
               
                   
                 ggtgctgggaaaactggacagccacatgtaaaaga 
               
               
                   
                   
               
               
                   
                 atgaaattagaatactccctaacaccatacacaaa 
               
               
                   
                   
               
               
                   
                 aataaactcaaaatggattagagacctaaatataa 
               
               
                   
                   
               
               
                   
                 gactggacactataaaactcttagaggaaaacata 
               
               
                   
                   
               
               
                   
                 ggaagaacactctttgacataaatcacagcaagat 
               
               
                   
                   
               
               
                   
                 ctttttcgatccacctcctagagtaatggaaataa 
               
               
                   
                   
               
               
                   
                 aaacaaaaataaacaagtgggacctaatgaaactt 
               
               
                   
                   
               
               
                   
                 caaagcttttgcacagcaaaggaaaccataaacaa 
               
               
                   
                   
               
               
                   
                 gacgaaaagacaaccctcagaatgggagaaaatat 
               
               
                   
                   
               
               
                   
                 ttgcaaatgaatcaacggacaaaggattaatctcc 
               
               
                   
                   
               
               
                   
                 aaaatatataaacagctcattcagctcaatatcaa 
               
               
                   
                   
               
               
                   
                 agaaacaaacaccccaatccaaaaatgggcagaag 
               
               
                   
                   
               
               
                   
                 acctaaatagacatttctccaaagaagacatacag 
               
               
                   
                   
               
               
                   
                 acggccacgaagcacatgaaaagatgctcaacatc 
               
               
                   
                   
               
               
                   
                 actaattattagagaaatgcaaatcaaaactacaa 
               
               
                   
                   
               
               
                   
                 tgaggtatcacctcactcctgttagaatgggcatc 
               
               
                   
                   
               
               
                   
                 atcagaaaatctacaaacaacaaatgctggagagg 
               
               
                   
                   
               
               
                   
                 gtgtggagaaaagggaaccctcttgcactgttggt 
               
               
                   
                   
               
               
                   
                 gggaatgtaaattgatacagccactatggagaaca 
               
               
                   
                   
               
               
                   
                 atatggaggttccttaaaaaactaaaaatagaatt 
               
               
                   
                   
               
               
                   
                 accatatgacccagcaatcccactactgggcatat 
               
               
                   
                   
               
               
                   
                 acccagagaaaaccgtaattcaaaaagacacatgc 
               
               
                   
                   
               
               
                   
                 acccgaatgttcattgcagcactatttacaatagc 
               
               
                   
                   
               
               
                   
                 caggtcatggaagcaacctaaatgcccatcgacag 
               
               
                   
                   
               
               
                   
                 acgaatggataaagaagatgtggtacatatataca 
               
               
                   
                   
               
               
                   
                 atggaatattactcagccataaaaaggaacgaaat 
               
               
                   
                   
               
               
                   
                 tgggtcatttttagagacgtggatggatctagaga 
               
               
                   
                   
               
               
                   
                 ctgtcatacagagtgaagtaagtcagaaagagaaa 
               
               
                   
                   
               
               
                   
                 aacaaatatcgtatattaacgcatatatgtggaac 
               
               
                   
                   
               
               
                   
                 ctggaaaaatggtacagatgaaccggtctgcagga 
               
               
                   
                   
               
               
                   
                 cagaaattgagacacaaatgtaa. 
               
            
           
         
       
     
     In some embodiments, the construct comprises a nucleic acid sequence encoding a nuclear localization sequence with at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to PAAKRVKLD (SEQ ID NO: 63). In some embodiments, the nuclear localization sequence is fused to the ORF2p sequence. In some embodiments, the construct comprises a nucleic acid sequence encoding a flag tag having the sequence DYKDDDDK (SEQ ID NO: 64). In some embodiments, the flag tag is fused to the ORF2p sequence. In some embodiments, the flag tag is fused to the nuclear localization sequence. 
     In some embodiments, the construct comprises a nucleic acid sequence encoding an MS2 coat protein with at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEV PKGAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIYAMASNFTQFVLVD NGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLN MELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIY (SEQ ID NO: 65). In some embodiments, the MS2 coat protein sequence is fused to the ORF2p sequence. 
     In some embodiments, the transgene may comprise a flanking sequence which comprises an Alu ORF2p recognition sequence. 
     In some embodiments, additional elements may be introduced into the mRNA. In some embodiments, the additional elements may be an IRES element or a T2A element. In some embodiments, the mRNA transcript comprises one, two, three or more stop codons at the 3′-end. 
     In some embodiments, the one, two, three or more stop codons are designed to be in tandem. In some embodiments, the one, two, three or more stop codons are designed to be in all three reading frames. In some embodiments, the one, two, three or more stop codons may be designed to be both in multiple reading frames and in tandem. 
     In some embodiments, one or more target specific nucleotides may be added at the priming end of the L1 or the Alu RNA priming region. 
     In some embodiments, the 5′ UTR sequence or the 3′ UTR sequence in addition to be able to bind the ORF protein may also be capable of binding to one or more endogenous proteins that regulate gene retrotransposition and/or stable integration. In some embodiments, the flanking sequence is capable of binding to a PABP protein. 
     In some embodiments, the 5′ region flanking the transcript may comprise a strong promoter. In some embodiments, the promoter is a CMV promoter. 
     In some embodiments, an additional nucleic encoding L1 ORF2p is introduced into the cell. In some embodiments, the sequence encoding L1 ORF1 is omitted, and only L1-ORF2 is included. In some embodiments, the nucleic acid encoding the transgene with the flanking elements is mRNA. In some embodiments, the endogenous L1-ORF1p function may be suppressed or inhibited. 
     In some embodiments, the nucleic acid encoding the transgene with the retrotransposition flanking elements comprise one or more nucleic acid modifications. In some embodiments, the nucleic acid encoding the transgene with the retrotransposition flanking elements comprises one or more nucleic acid modifications in the transgene. In some embodiments, the modifications comprise codon optimization of the transgene sequence. In some embodiments, the codon optimization is for more efficient recognition by the human translational machinery, leading to more efficient expression in a human cell. In some embodiments, the one or more nucleic acid modification is performed in the 5′-flanking sequence or the 3′-flanking sequence including one or more stem-loop regions. the nucleic acid encoding the transgene with the retrotransposition flanking elements comprise one, two, three, four, five, six, seven eight, nine, ten or more nucleic acid modifications. 
     In some embodiments, the retrotransposed transgene is stably expressed for the life of the cell. In some embodiments, the cell is a myeloid cell. In some embodiments, the myeloid cell is a monocyte precursor cell. In some embodiments, the myeloid cell is an immature monocyte. In some embodiments, the monocyte is an undifferentiated monocyte. In some embodiments, the myeloid cell is a CD14+ cell. In some embodiments, the myeloid cell does not express CD16 marker. In some embodiments, the myeloid cell is capable of remaining functionally active for a desired period of greater than 3 days, greater than 4 days, greater than 5 days, greater than 6 days, greater than 7 days, greater than 8 days, greater than 9 days, greater than 10 days, greater than 11 days, greater than 12 days, greater than 13 days, greater than 14 days or more under suitable conditions. A suitable condition may denote an in vitro condition, or an in vivo condition or a combination of both. 
     In some embodiments, the retrotransposed transgene may be stably expressed in the cell for about 2 days, about 3 days, about 4 days, about 5 days, about 6 days, about 7 days, about 8 days, about 9 days or about 10 days. In some embodiments, the retrotransposed transgene is stably expressed in the cell for more than 10 days. In some embodiments, the retrotransposed transgene is stably expressed in the cell for more than 2 weeks. In some embodiments, the retrotransposed transgene is stably expressed in the cell for about 1 month. 
     In some embodiments, the retrotransposed transgene may be modified for stable expression. In some embodiments, the retrotransposed transgene may be modified for resistant to in vivo silencing. 
     In some embodiments, the expression of the retrotransposed transgene may be controlled by a strong promoter. In some embodiments, the expression of the retrotransposed transgene may be controlled by a moderately strong promoter. In some embodiments, the expression of the retrotransposed transgene may be controlled by a strong promoter that can be regulated in an in vivo environment. In some embodiments, the promoter is a CMV promoter. In some embodiments, the promoter is a L1-Ta promoter. 
     In some embodiments, the ORF1p may be overexpressed. In some embodiments, the ORF2 may be overexpressed. In some embodiments, the ORF1p or ORF2p or both are overexpressed. In some embodiments, upon overexpression of an ORF1, ORF1p is at least 1.1 fold, 1.5 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, 10 fold, 12 fold, 14 fold, 16 fold, 18 fold, 20 fold, 30 fold, 40 fold, 50 fold, 60 fold, 70 fold, 80 fold, 90 fold, or at least 100 fold higher than a cell not overexpressing and ORF1. 
     In some embodiments, upon overexpression of an ORF2 sequence, ORF2p is at least 1.1 fold, 1.5 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, 10 fold, 12 fold, 14 fold, 16 fold, 18 fold, 20 fold, 30 fold, 40 fold, 50 fold, 60 fold, 70 fold, 80 fold, 90 fold, or at least 100 fold higher than a cell not overexpressing and ORF2p. 
     Retrotransposition Fidelity and Target Specificity 
     The LINE-1 elements can bind to their own mRNA poly A tail to initiate retrotransposition. LINE-1 elements preferably retrotranspose their own mRNA over random mRNAs (Dewannieux et al., 2013, 3,000-fold higher LINE-1 retrotransposition as compared to random mRNAs). In addition, LINE-1 elements can also integrate non-specific poly-A sequences within a genome. 
     In one aspect, provided herein are retrotransposition compositions and methods of using the same with increased retrotransposition specificity. For example, retrotransposition compositions with high specificity may be used for highly specific and efficient reverse transcription and subsequently, integration into genome of a target cell, e.g., a myeloid cell. In some embodiments, a retrotransposition composition provided herein comprises a retrotransposition cassette that comprises one or more additional components that increases integration or retrotransposing specificity. For example, the retrotransposon cassette may encode one or more additional elements that allows for high affinity RNA-protein interaction to out compete non-specific binding between poly-A sequences and ORF2. 
     Accordingly, several measures are disclosed herein for enhancing integration or retrotransposition efficiency. 
     One exemplary measure for enhancing integration or retrotransposition efficiency is external manipulation of the cells. The endonuclease function of the retrotransposition machinery delivered in a cell may likely be subject to inhibition by the cell&#39;s transposition silencing machinery, such as DNA repair pathways. For example, small molecules can be used to modulate or inhibit DNA repair pathways in the cells prior to introducing the nucleic acid. For example, cell sorting and/or synchronization can be used prior to introducing the nucleic acid, such as by electroporation, as cell cycle synchronized cell populations were shown to increase gene transfer to the cells. Cell sorting may be utilized to synchronize or homogenize the cell types and increase uniform transfer and expression of the exogenous nucleic acid. Uniformity may be achieved sorting stem cells from non-stem cells. Another exemplary measure for enhancing integration or retrotransposition efficiency is to enhance biochemical activity. For example, this may be achieved by increasing reverse-transcriptase processivity or DNA cleavage (endonuclease) activity. Another exemplary measure for enhancing integration or retrotransposition efficiency is to subvert endogenous silencing mechanisms. For example, this may be achieved by replacing entire LINE-1 sequence with a different organisms&#39; LINE-1. Another exemplary measure for enhancing integration or retrotransposition efficiency is to enhance translation and ribosome binding. For example, this may be achieved by increasing expression of LINE-1 proteins, increasing LINE protein binding LINE-1 mRNA, or increasing LINE-1 complex binding to ribosomes. Another exemplary measure for enhancing integration or retrotransposition efficiency is to increase nuclear import or retention. For example, this may be achieved by fusing the LINE-1 sequence to a nuclear retention signal sequence. Another exemplary measure for enhancing integration or retrotransposition efficiency is to enhance sequence-specific insertion. For example, this may be achieved by fusing a targeting domain to ORF2 to increase sequence specific retrotransposition. 
     In one embodiment, the method encompasses enhancing the retrotransposon for increasing specificity and robustness of expression of the cargo by modifying the UTR sequence of the LINE-1 ORFs. In some embodiments, the 5′UTR upstream of ORF1 or ORF2 encoding sequence may be further modified to comprise a sequence that is complementary to the sequence of a target region within the genome that helps in homologous recombination at the specific site where the ORF nuclease can act and the retrotransposition can take place. In some embodiments, the sequence that can bind to a target sequence by homology is between 2-15 nucleotides long. In some embodiments, the sequence having homology to a genomic target that is included in the 5′UTR of an ORF1 mRNA may be about 3 nucleotides, about 4 nucleotides, about 5 nucleotides, about 6 nucleotides, about 7 nucleotides, about 8 nucleotides, about 9 nucleotides or about 10 nucleotides long. In some embodiments, the sequence having homology to a genomic target is about 12 or about 15 nucleotides long. In some embodiments, the sequence having homology to a genomic target is at least about 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 1120 or 125 nucleotides in length. In some embodiments, the sequence having homology to a genomic target comprises about 2-5, about 2-6, about 2-8 or about 2-10, or about 2-12 contiguous nucleotides that share complementarity with the respective target region within the genome. In some embodiments, the sequence having homology to a genomic target is at least about or about 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 1120 or 125 contiguous nucleotides that share complementarity with the respective target region within the genome. 
     In some embodiments, an ORF2 is associated with or fused to an additional protein domain that comprises RNA binding activity. In some embodiments, the retrotransposon cassette comprises a cognate RNA sequence that comprises affinity with the additional protein domain associated with or fused to the ORF2. In some embodiments, the ORF2 is associated with or fused to a MS2-MCP coat protein. In some embodiments, the retrotransposon cassette further comprises a MS2 hairpin RNA sequence in the 3′ or 5′ UTR sequence that interacts with the MS2-MCP coat protein. In some embodiments, the ORF2 is associated with or fused to a PP7 coat protein. In some embodiments, the retrotransposon cassette further comprises a PP7 hairpin RNA sequence in the 3′ or 5′ UTR sequence that interacts with the MS2-MCP coat protein. In some embodiments, the one or more additional elements increases retrotransposition specificity by at least 1.5 fold, at least 2 fold, at least 3 fold, at least 4 fold, at least 5 fold, at least 10 fold, at least 20 fold, at least 30 fold, at least 50 fold, at least 100 fold, at least 200 fold, at least 300 fold, at least 500 fold, at least 1000 fold, at least 1500 fold, at least 2000 fold, at least 3000 fold, at least 5000 fold or more as compared to a retrotransposon cassette without the one or more additional elements. 
     The DNA endonuclease domain appears to have specificity for a series of purines 3′ of the target site followed by a series of pyrimidines (Py) n ↓(Pu) n . An exemplary sequence may be (Adenosine) n ↓(Thymidine) n . 
     In one aspect, provided herein are methods of using retrotransposition having high target specificity. In some embodiments, a CRISPR-Cas guide RNA system is combined with the LINE-retrotransposon system used here to increase the precision of site directed retrotransposition. In some embodiments, the system incorporates a prime editing guide RNA (pegRNA) to incorporate one or more ORF-binding sequence into a specific genomic locus. In some embodiments, the pegRNA incorporates the sequence that binds human ORF, e.g. TTTTTA in a site-specific manner. In some embodiments, the CRISPR-Cas system comprises a Cas9 enzyme. In some embodiments the CRISPR-Cas comprises a Cfp1 enzyme. In some embodiments, the Cas9 is a dCas9, paired with a nickase system. 
     Consequently, provided herein is a method and compositions for stable incorporation of a transgene into the genome of a myeloid cell, such as a monocyte or macrophage, wherein the method comprises incorporating the transgene using a non-LTR retrotransposon system, wherein the retrotransposition occurs at a specific genomic locus with a target specificity, high precision and fidelity. Therefore, in some embodiments, the method comprises administration to the cell a composition comprising a system having at least one transgene, flanked with one or more retrotransposable elements, and one or more nucleic acids encoding one or more proteins for increasing the transposition specificity, and/or further comprising modifying one or more genes associated with the retrotransposition. 
     The nucleic acid comprising the transgene, situated in 3′ UTR region of the retrotransposable elements is often referred to as a retrotransposition cassette. Accordingly, in some embodiments, the retrotransposition cassette comprises the nucleic acid encoding the transgene and flanking Alu transposable elements. The retrotransposable elements comprise a sequence for binding the retrotransposons, for example, L1-transposons, such as L1-ORF proteins, ORF1p and ORF2p. ORF proteins are known to bind to their own mRNA sequence for retrotransposition. Therefore, the retrotransposition cassette comprises the nucleic acid encoding the transgene; a flanking L1-ORF2p binding sequence, and/or a L1-ORF1p binding sequence, comprising a sequence encoding a L1-ORF1p encoding sequence and a L1-ORF2p encoding sequence outside the transgene sequence. In some embodiments, the L1-ORF1 and L1-ORF2 are interspersed by a spacer region, also termed as an ORF1-ORF2 inter-region. In some embodiments, the L1-ORF1 and L1-ORF2 coding sequences are in an opposite orientation with respect to the coding region of the transgene. The retrotransposition cassette can comprise a poly A region downstream of the L1-ORF2-coding sequence and the transgene sequence is placed downstream of the poly A sequence. The L1-ORF2 comprises a nucleic acid sequence that encodes an endonuclease (EN) and a reverse transcriptase (RT) followed by the poly A sequence. In some embodiments, the L1-ORF2 sequence in the retrotransposition cassette described herein is a complete (intact) sequence, that is, encodes the full length native (WT) L1-ORF2 sequence. In some embodiments, the L1-ORF2 sequence in the retrotransposition cassette described herein comprises a partial or modified sequence. 
     The system described herein can comprise a promoter for expressing the L1-ORF1p and L1-ORF2p. In some embodiments, the transgene expression is driven by a separate promoter. In some embodiments, the transgene and the ORFs are in tandem orientation. In some embodiments, the transgene and the ORFs are in opposite orientation. 
     In some embodiments, the method comprises incorporating one or more elements in addition to the retrotransposon cassette. In some embodiments, the one or more additional elements comprise a nucleic acid sequence encoding one or more domains of a heterologous protein. The heterologous protein may be a sequence specific nucleic acid binding protein, for example, a sequence specific DNA binding protein domain (DBD). In some embodiments, the heterologous protein is a nuclease or a fragment thereof. In some embodiments, the additional elements comprise a nucleic acid sequence encoding one or more nuclease domains or fragments thereof from a heterologous protein. In some embodiments, the heterologous nuclease domain has reduced nuclease activity. In some embodiments, the heterologous nuclease domain is rendered inactive. In some embodiments, the ORF2 nuclease is rendered inactive; whereas one or more nuclease domains from the heterologous protein is configured to render specificity to the retrotransposition. In some embodiments, one or more nuclease domains or fragments thereof from the heterologous protein targets a specific desired polynucleotide within the genome where retrotransposition and incorporation of the polynucleotide of interest is to be incorporated. In some embodiments, the one or more nuclease domains from the heterologous protein comprise a mega-TAL nuclease domain, TALENs, or a zinc finger nuclease domain, for example, a mega-TAL, a TALE, or a zinc finger domain fused to or associated with a nuclease domain, e.g., a FokI nuclease domain. In some embodiments, the one or more nuclease domains from the heterologous protein comprise a CRISPR-Cas protein domain loaded with a specific guide nucleic acid, e.g., a guide RNA (gRNA) for a specific target locus. In some embodiments, the CRISPR-Cas protein is a Cas9, a Cas12a, a Cas12b, a Cas13, a CasX, or a CasY protein domain. In some embodiments, the one or more nuclease domains from the heterologous protein has target specificity. 
     In some embodiments, the additional nuclease domain may be incorporated into the ORF2 domain. In some embodiments, the additional nuclease may be fused with the ORF2p domain. In some embodiments, the additional nuclease domain may be fused to an ORF2p, wherein the ORF2p includes a mutation in the ORF2p endonuclease domain. In some embodiments, the mutation inactivates the ORF2p endonuclease domain. In some embodiments, the mutation is a point mutation. In some embodiments, the mutation is a deletion. In some embodiments, the mutation is an insertion. In some embodiments, the mutation abrogates the ORF2 endonuclease (nickase) activity. In some embodiments, a mutation inactivates the DNA target recognition of ORF2p endonuclease. In some embodiments, the mutation covers a region associated with ORF2p nuclease-DNA recognition. In some embodiments, a mutation reduces the DNA target recognition of ORF2p endonuclease. In some embodiments, the ORF2p endonuclease domain mutation is in the N-terminal region of the protein. In some embodiments, the ORF2p endonuclease domain mutation is in a conserved region of the protein. In some embodiments, the ORF2p endonuclease domain mutation is in the conserved N-terminal region of the protein. In some embodiments, the mutation comprises the N14 amino acid within L1 endonuclease domain. In some embodiments, the mutation comprises 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or more consecutive amino acids including the N14 amino acid within L1 endonuclease domain. In some embodiments, the mutation comprises the comprises the E43 amino acid within L1 endonuclease. In some embodiments, the mutation comprises 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or more consecutive amino acids including the E43 amino acid within L1 endonuclease domain. In some embodiments, the mutation comprises 2 or more amino acids in the L1 endonuclease domain including N14, or E43 or a combination thereof. In some embodiments, the mutation comprises D145 of the L1 endonuclease domain. In some embodiments, the mutation may be D145A. In some embodiments, the may be a comprise D205 of the L1 endonuclease domain. In some embodiments, the mutation may be D205G. In some embodiments, the mutation may comprise H230 of L1 endonuclease domain. In some embodiments, the may be a comprise S228 of the L1 endonuclease domain. In some embodiments, the mutation may be S228P. 
     In some embodiments, a mutation reduces the DNA target recognition of ORF2p endonuclease by at least 50%. In some embodiments, a mutation reduces the DNA target recognition of ORF2p endonuclease by at least 60%. In some embodiments, a mutation reduces the DNA target recognition of ORF2p endonuclease by at least 70%. In some embodiments, a mutation reduces the DNA target recognition of ORF2p endonuclease 80%. In some embodiments, a mutation reduces the DNA target recognition of ORF2p endonuclease 90%. In some embodiments, a mutation reduces the DNA target recognition of ORF2p by 95%. In some embodiments, a mutation reduces the DNA target recognition of ORF2p by 100%. 
     In some embodiments, the mutation is a deletion. In some embodiments, the deletion is complete, i.e., 100% of the L1 endonuclease domain is deleted. In some embodiments, the deletion is partial. In some embodiments, the about 98%, about 95%, about 94%, about 93%, about 92% about 91%, about 90%, about 85%, about 80%, about 75%, about 70%, about 65%, about 60%, or about 50% of the ORF2 endonuclease domain is deleted. 
     In some embodiments, an additional nuclease domain is inserted into the ORF2 protein sequence. In some embodiments, ORF2 endonuclease domain is deleted, and is replaced with an endonuclease domain from a heterologous protein. In some embodiments, the ORF2 endonuclease is partially deleted and replaced with an endonuclease domain from a heterologous protein. The endonuclease domain from a heterologous protein may be a mega-TAL nuclease domain. The endonuclease domain from a heterologous protein may be a TALENs. The endonuclease domain from a heterologous protein may be a Cas9 loaded with a specific gRNA for a locus. 
     In some embodiments, the endonuclease is an endonuclease that has (i) a specific target on the genome and (ii) it creates a 5′-P and a 3′-OH terminus at the cleavage site. 
     In some embodiments, the additional endonuclease domain from a heterologous protein is an endonuclease domain from a related retrotransposon. 
     In some embodiments, the endonuclease domain from a heterologous protein may comprise a bacterial endonuclease engineered for targeting a specific site. In some embodiments, the endonuclease domain from a heterologous protein may comprise a domain of a homing endonuclease or a fragment thereof. In some embodiments, the endonuclease is a homing endonuclease. In some embodiments, the homing endonuclease is an engineered LAGLIDADG homing endonucleases (LHEs) (“LAGLIDADG” disclosed as SEQ ID NO: 66) or a fragment thereof. In some embodiments, additional endonucleases may be a restriction endonuclease, Cre, Cas TAL or fragments thereof. In some embodiments, the endonuclease may comprise a Group II intron encoded protein (ribozyme) or a fragment thereof. 
     An engineered or modified L1-ORF2p as discussed in the preceding paragraphs, that is endowed with specific DNA targeting capability due to the additional/heterologous endonuclease is expected to be highly advantageous in driving targeted stable integration of a transgene into the genome. The engineered L1-ORF2p can generate much reduced off-target effects when expressed in a cell than using a native, non-engineered L1-ORF2p. In some embodiments, the engineered L1-ORF2p generates no off-target effect. 
     In some embodiments, the engineered or modified L1-ORF2p targets a recognition site that is other than the usual (Py) n ↓(Pu) n  site. In some embodiments, engineered L1-ORF2p targets a recognition site that comprises the (Py) n ↓(Pu) n  site, for example, TTTT/AA site, such as a hybrid target site. In some embodiments, the engineered L1-ORF2p targets a recognition site having at least one nucleotide in addition to the conventional L1-ORF2 (Py) n ↓(Pu) m  site, for example TTTT/AAG, or TTTT/AAC, or TTTT/AAT, TTTT/AAA, GTTTT/AA, CTTTT/AA, ATTTT/AA, or TTTTT/AA. In some embodiments, the engineered L1-ORF2p targets a recognition site that is in addition to the conventional L1-ORF2p (Py) n ↓(Pu) n  site. In some embodiments, the engineered L1-ORF2p targets a recognition site that is other than to the conventional L1-ORF2p (Py) n ↓(Pu) n  site. In some embodiments, the engineered L1-ORF2p targets a recognition site that is 4, 5, 6, 7, 8, 9, 10 or more nucleotides long. In some embodiments, the engineered or modified L1-ORF2p recognition site may be 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or more nucleotides. 
     The engineered L1-ORF2p can be engineered to retain its ability to bind to its own mRNA after translation and reverse transcribe with high efficiency. In some embodiments, the engineered L1-ORF2p has enhanced efficiency of reverse transcription compared to a native (WT) L1-ORF2p. 
     In some embodiments, the system comprising a retrotransposable element further comprises a gene modification that reduces non-specific retrotransposition. In some embodiments, the gene modification may comprise a sequence encoding the L1-ORF2p. In some embodiments, the modification may comprise mutation of one or more amino acids that are essential for binding to a protein that helps ORF2p binding to the target genomic DNA. A protein that helps ORF2p binding to the target genomic DNA may be part of the chromatin-ORF interactome. In some embodiments, the modification may comprise one or more amino acids that are essential for binding to a protein that helps ORF2p DNA endonuclease activity. In some embodiments, the modification may comprise one or more amino acids that are essential for binding to a protein that helps ORF2p RT activity. In some embodiments, the modification may comprise at a protein binding site on ORF2p such that the association of a protein with ORF2p is altered, wherein binding of the protein to ORF2p is required for binding to chromatin. In some embodiments, the modification may comprise at a protein binding site on ORF2p such that the association of the protein with ORF2p is more stringent and/or specific than in absence of the modification. In some embodiments, as a consequence of altered association of ORF2p with the protein owing to the modification of ORF2p coding sequence at the protein binding site, the binding of ORF2p to the target DNA has increased specificity. In some embodiments, the modification may reduce binding of ORF2 to one or more proteins that are part of the ORF2p chromatin interactome. 
     In some embodiments, the gene modification may be in the PIP domain of ORF2p. 
     In some embodiments, the gene modification may be in one or more genes encoding a protein that binds to an ORF2p and helps in the recognition, binding, endonuclease or RT activity of ORF2p. In some embodiments, the gene modification may be in one or more genes encoding PCNA, PARP1, PABP, MCM, TOP1, RPA, PURA, PURB, RUVBL2, NAP1, ZCCHC3, UPF1 or MOV10 proteins at an ORF2p interacting site for each protein or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the modification may be on an ORF2p binding domain of PCNA at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the modification may be on an ORF2p binding domain of TOP1. In some embodiments, the modification may be on an ORF2p binding domain of RPA. In some embodiments, the modification may be on an ORF2p binding domain of PARP1 at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the modification may be on an ORF2p binding domain of PABP (e.g., PABPC1) at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the gene modification may be on an MCM gene. In some embodiments, the gene modification may be on a gene encoding MCM3 protein at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the gene modification may be on a gene encoding MCM5 protein at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the gene modification may be on a gene encoding MCM6 protein at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the gene modification may be on a gene encoding MEPCE protein at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the gene modification may be on a gene encoding on a gene encoding RUVBL1 or RUVBL2 protein at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. In some embodiments, the gene modification may be on a gene encoding on a gene encoding TROVE protein at an ORF2p interacting site or at a site that affects the protein&#39;s interaction with ORF2p or the interaction of ORF2p with target DNA. 
     In some embodiments, the retrotransposition system disclosed herein comprises one or more elements that increase the fidelity of reverse transcription. 
     In some embodiments, the L1-ORF2 RT domain is modified. In some embodiments, the modification includes one or more of: increasing fidelity, increasing processivity, increasing DNA-RNA substrate affinity; or inactivating RNase H activity. 
     In some embodiments, the modification comprises introducing one or more mutations in the RT domain of the L1-ORF2, such that the fidelity of the RT is increased. In some embodiments, the mutation comprises a point mutation. In some embodiments, the mutation comprises alteration, such as substitution of one, two three, four, five, six or more amino acids in the L1-ORF2p RT domain. In some embodiments, the mutation comprises deletion of one or more amino acids, for example, one, two, three, four, five, six, seven, eight, nine, ten or more amino acids in the L1-ORF2p RT domain. In some embodiments, the mutation may comprise an in-del mutation. In some embodiments, the mutation may comprise a frame-shift mutation. 
     In some embodiments, the modification may comprise inclusion of an additional RT domain or fragment thereof from a second protein. In some embodiments, the second protein is a viral reverse transcriptase. In some embodiments, the second protein is a non-viral reverse transcriptase. In some embodiments, the second protein is a retrotransposable element. In some embodiments, the second protein is a non-LTR retrotransposable element. In some embodiments, the second protein is a group II intron protein. In some embodiments, the group II intron is as TGIRTII. In some embodiments, the second protein is a Cas nickase, wherein the retrotransposable system further comprises introducing a guide RNA. In some embodiments, the second protein is a Cas9 endonuclease, wherein the retrotransposable system further comprises introducing a guide RNA. In some embodiments, the second protein or fragment thereof is fused to the N-terminus of the L1-ORF2 RT domain or the modified L1-ORF2 RT domain. In some embodiments, the second protein or fragment thereof is fused to the C-terminus of the L1-ORF2 RT domain or the modified L1-ORF2 RT domain. 
     In some embodiments, the additional RT domain or fragment thereof from the second protein is incorporated in the retrotransposition system in addition to the full-length WT L1-ORF2p RT domain. In some embodiments, the additional RT domain or fragment thereof from the second protein is incorporated in presence of a modified (engineered) L1-ORF2p RT domain or a fragment thereof, where the modification (or engineering) may comprise a mutation for enhancement of the L1-ORF2p RT processivity, stability and/or fidelity of the modified L1-ORF2p RT compared to the native or WT ORF2p. 
     In some embodiments, the reverse transcriptase domain could be replaced with other more highly processive and high-fidelity RT domains from other retroelements or group II introns, such as TGIRTII. 
     In some embodiments, the modification may comprise a fusion with an additional RT domain or fragment thereof from a second protein. In some embodiments, the second protein may comprise a retroelement. The additional RT domain or fragment thereof from a second protein is configured to increase the fidelity of reverse transcription of the fused L1-ORF2p RT domain. In some embodiments, the nucleic acid encoding the additional RT domain or fragment thereof is fused to a native or WT L1-ORF2 encoding sequence. In some embodiments, the nucleic acid encoding the additional RT domain or fragment thereof from a second protein is fused to a modified L1-ORF2 encoding sequence. In some embodiments, the modification comprises introducing one or more mutations in the RT domain of the L1-ORF2 or fragment thereof, such that the fidelity of the fused RT is increased. In some embodiments, the mutation in the RT domain of the L1-ORF2 or fragment thereof comprises a point mutation. In some embodiments, the mutation comprises alteration, such as substitution of one, two three, four, five, six or more amino acids in the L1-ORF2p RT domain. In some embodiments, the mutation comprises deletion of one or more amino acids, for example, one, two, three, four, five, six, seven, eight, nine, ten or more amino acids in the L1-ORF2p RT domain. In some embodiments, the mutation may comprise an in-del mutation. In some embodiments, the mutation may comprise a frame-shift mutation. 
     In some embodiments, the modified L1-ORF2p RT domain has increased processivity than the WT L1-ORF2p RT domain. 
     In some embodiments, the modified L1-ORF2p RT domain has at least 10% higher processivity and/or fidelity over the WT L1-ORF2p RT domain. In some embodiments, the modified L1-ORF2p RT domain has at least 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 110%, 120%, 150%, 200%, 300%, 400%, 500%, 1000% or higher processivity and/or fidelity over the WT L1-ORF2p RT domain. In some embodiments, the modified RT can process greater than 6 kb nucleic acid stretch. In some embodiments, the modified RT can process greater than 7 kb nucleic acid stretch. In some embodiments, the modified RT can process greater than 8 kb nucleic acid stretch. In some embodiments, the modified RT can process greater than 9 kb nucleic acid stretch. In some embodiments, the modified RT can process greater than 10 kb nucleic acid stretch. 
     B. Group II Introns and Ribozymes 
     Group II enzymes are mobile ribozymes that self-splice precursor RNAs, yielding excised intron lariat RNAs. The introns encode a reverse transcriptase. The reverse transcriptase may stabilize the RNA for forward and reverse splicing, and later in converting the integrated intron RNA to DNA. 
     Group II RNAs are characterized by a conserved secondary structure spanning 400-800 nucleotides. The secondary structure is formed by six domains DI-VI, and is organized in a structure resembling a wheel, where the domains radiate from a central point. The domains interact to form a conserved tertiary structure that brings together distant sequences to form an active site. The active site binds the splice sites and branch point residue nucleotide and in association of Mg2+ cations, activate catalysis of splicing. The DV domain is within the active site, which has the conserved catalytic AGC and an AY bulge and both these regions bind Mg2+ ions necessary for the catalysis. DI is the largest domain with upper and lower halves separated by kappa and zeta motifs. The lower half contains the ε′ motif, which is associated with an active site. The upper half contains sequence elements that bind to the 5′ and 3′ exons at the active sites. DIV encodes the intron-encoded protein (IEP) with subdomain IVa near the 5′-end containing the high affinity binding site for IEP. Group II introns have conserved 5′- and 3′-end sequences, GUGYG and AY respectively. 
     Group II RNA introns can be utilized to retrotranspose a sequence of interest into DNA via target primed reverse transcription. This process of transposition by Group II RNA introns is often referred to as retrohoming. Group II introns recognize DNA target sites by base pairing of the intron RNA to the DNA target sequence, they can be modified to retarget a specific sequence carried within the intron to a desired DNA site. 
     In some embodiments, the method and compositions for retrotransposition described herein may comprise a Group II intron sequence, a modified Group II intron sequence or a fragment thereof. Exemplary Group II IEPs (maturase) include but are not limited to bacterial, fungal, yeast IEPs, that are functional in human cells. In particular, the nuclease leaves a 3′-OH at the cleavage site of the DNA which can be utilized by another RT for priming and reverse transcription. An exemplary Group II maturase may be TGIRT (thermally stable group II intron maturase). 
     In one or more embodiments of several aspects described herein, the nucleic acid construct comprises an RNA. In one or more embodiments of several aspects of the disclosure, the nucleic acid construct is an RNA. In one or more embodiments of several aspects of the disclosure, the nucleic acid construct is an mRNA. In one aspect, the mRNA comprises a sequence of a heterologous gene or portion thereof, wherein the heterologous gene or portion thereof encodes a polypeptide or protein. In some embodiments, the mRNA comprises a sequence encoding a fusion protein. In some embodiments, the mRNA comprises a sequence encoding a recombinant protein. In some embodiments, the mRNA comprises a sequence encoding a synthetic protein. In some embodiments, the nucleic acid comprises one or more sequences, wherein the one or more sequences encode on or more heterologous proteins, one or more recombinant proteins, or one or more synthetic proteins or a combination thereof. In some embodiments, the nucleic acid comprises one or more sequences, wherein the one or more sequences encode on or more heterologous proteins comprising a synthetic protein or a recombinant protein. In some embodiments, the synthetic or recombinant protein is a recombinant fusion protein. 
     C. Retrotransposon Systems Comprising an Site Directed Editing and/or Integrase 
     In one aspect, provided herein are methods of using retrotransposition having high target specificity following modification over the pegRNA mediated incorporation of LINE binding sequences site-specifically into the genome, with the help of guide RNA and a Cas protein. In some embodiments, a CRISPR-Cas guide RNA system is combined with the LINE-retrotransposon system used here to increase the precision of site directed retrotransposition; for example, the system incorporates a prime editing guide RNA (pegRNA) to incorporate one or more ORF-binding sequence into a specific genomic locus. In some embodiments, the pegRNA incorporates the sequence that binds human ORF, e.g. TTTTTA in a site-specific manner. In some embodiments, the CRISPR-Cas system comprises a Cas9 enzyme. In some embodiments the CRISPR-Cas comprises a Cfp1 enzyme. In some embodiments, the Cas9 is a dCas9, paired with a nickase system. 
     In some embodiments, the retrotransposon systems described herein comprise (i) a LINE1 retrotransposon element, and (ii) an integrase system or parts thereof. Some integrase systems are capable of site-specific integration—of double stranded DNA. In order to bypass the double stranded DNA delivery and/or integration into a genome, provided herein is a recombinant hybrid system wherein the integrase or a fragment thereof incorporated within a recombinant ORF protein, or delivered separately as a separate nucleic acid (e.g. mRNA) encoding an integrase or fragment thereof that recognizes a specific genomic site; and couples with the LINE 1 reverse transcription and insertion of a cargo sequence within the genome of a cell or an organism at the precise location led by the specificity of the integrase. This could be achieved in a few alternative ways. In some embodiments, the cargo sequence comprises an attachment site that is recognized and utilized by the integrase to draw the cargo to the landing site within the genome, also recognized by the same integrase. The integrase is capable of a single strand cut. The integrase DNA recognition site, i.e., the genomic landing sequence, can be 10 nucleotides long, e.g., 12, 14, 16, 18, 20, 22, 24, 26, 28, 30 or more nucleotides long, thereby conferring greater specificity than any other system. The integrase may be truncated or otherwise mutated to allow the ORF to reverse transcribe and integrate the cargo sequence at the integrase specified genomic site. Conversely, the ORF protein may also be mutated at the RNA recognition site to allow the integrase recognize the genomic integration sequence preferentially recognized by the integrase (also termed the “genomic landing sequence or site”. In alternative embodiments, the integrase is encoded by a separate polynucleotide, and may be driven by a CRISPR Cas system and a guide RNA to a site that can be nicked and an integrase landing sequence further comprising an ORF binding site comprising the 4 nucleotides may be introduced, thereafter the integrase draws in the cargo sequence that comprises the attachment sequence to the landing sequence, followed by the LINE1 activity leading to genomic integration at the site specified by the integrase system. Any catalytic activity of the integrase leading to double-stranded DNA incorporation in the genome is mutated or truncated, otherwise, silenced. 
     In one or more of embodiments of several aspects of the disclosure, the nucleic acid construct is developed for expressing in a eukaryotic cell. In some embodiments, the nucleic acid construct is developed for expressing in a human cell. In some embodiments, the nucleic acid construct is developed for expressing in a hematopoietic cell. In some embodiments, the nucleic acid construct is developed for expressing in a myeloid cell. In some embodiments, the myeloid cell is a human cell. 
     II. Modifications in Nucleic Acid Constructs for Methods of Enhancement of Expression of Encoded Protein 
     In some aspects of the disclosure, the recombinant nucleic acid is modified for enhanced expression of the protein encoded by a sequence of the nucleic acid. Enhanced expression of the protein encoded therein can be a function of the nucleic acid stability, translation efficiency and the stability of the translated protein. A number of modifications are contemplated herein for incorporation in the design of the nucleic acid construct that can confer nucleic acid stability, such as stability of the messenger RNA encoding the exogenous or heterologous protein, which may be a synthetic recombinant protein or a fragment thereof. 
     In some embodiments, the nucleic acid is mRNA, comprising one or more sequences, wherein the one or more sequences encode one or more heterologous proteins comprising a synthetic or a recombinant fusion protein. 
     In some embodiments, one or more modifications are made in the mRNA comprising a sequence encoding a recombinant or fusion protein to increase the mRNA half-life. 
     Structural Elements to Block 5′- and 3′-Degradations by Exonucleases: 5′-Cap and 3′ UTR Modifications 
     A proper 5′-cap structure is important in the synthesis of functional messenger RNA. In some embodiments, the 5′-cap comprises a guanosine triphosphate arranged as GpppG at the 5′ terminus of the nucleic acid. In some embodiments, the mRNA comprises a 5′ 7-methylguanosine cap, m7-GpppG. A 5′ 7-methylguanosine cap increases mRNA translational efficiency and prevents degradation of mRNA 5′-3′ exonucleases. In some embodiments, the mRNA comprises “anti-reverse” cap analog (ARCA, m 7,3′-O  GpppG). Translational efficiency, however, can be markedly increased by usage of the ARCA. In some embodiments, the guanosine cap is a Cap 0 structure. In some embodiments, the guanosine cap is a Cap 1 structure. In addition to its essential role of cap-dependent initiation of protein synthesis, the mRNA cap also functions as a protective group from 5′ to 3′ exonuclease cleavage and a unique identifier for recruiting protein factors for pre-mRNA splicing, polyadenylation and nuclear export. It acts as the anchor for the recruitment of initiation factors that initiate protein synthesis and the 5′ to 3′ looping of mRNA during translation. Three enzymatic activities are required to generate the Cap 0 structure, namely, RNA triphosphatase (TPase), RNA guanylyltransferase (GTase) and guanine-N7 methyltransferase (guanine-N7 MTase). Each of these enzyme activities carries out an essential step in the conversion of the 5′ triphosphate of nascent RNA to the Cap 0 structure. RNA TPase removes the γ-phosphate from the 5′ triphosphate to generate 5′ diphosphate RNA. GTase transfers a GMP group from GTP to the 5′ diphosphate via a lysine-GMP covalent intermediate. The guanine-N7 MTase then adds a methyl group to the N7 amine of the guanine cap to form the cap 0 structure. For Cap 1 structure, m7G-specific 2′O methyltransferase (2′O MTase) methylates the +1 ribonucleotide at the 2′O position of the ribose to generate the cap 1 structure. The nuclear RNA capping enzyme interacts with the polymerase subunit of RNA polymerase II complex at phosphorylated Ser5 of the C-terminal heptad repeats. RNA guanine-N7 methyltransferase also interacts with the RNA polymerase II phosphorylated heptad repeats. In some embodiments, the cap is a G-quadruplex cap. 
     In some embodiments, the mRNA is synthesized by in vitro transcription (IVT). In some embodiments, mRNA synthesis and capping may be performed in one step. Capping may occur in the same reaction mixture as IVT. In some embodiments, mRNA synthesis and capping may be performed in separate steps. mRNA thus formed by IVT is purified and then capped. 
     In some embodiments, the nucleic acid construct, e.g., the mRNA construct, comprises one or more sequences encoding a protein or a polypeptide of interest can be designed to comprise elements that protect, prevent, inhibit or reduce degradation of the mRNA by endogenous 5′-3′ exoribonucleases, for example, Xrn1. Xrn1 is a cellular enzyme in the normal RNA decay pathways that degrades 5′ monophosphorylated RNAs. However, some viral RNA structural elements are found to be particularly resistant to such RNases, for example, the Xrn1-resistant structure in flavivirus sfRNAs, called the ‘xrRNA’. For example, the mosquito-borne flaviviruses (MBFV) genomes contain discrete RNA structures in their 3′-untranslated region (UTR) that block the progression of Xrn1. These RNA elements are sufficient to block Xrn1 without the use of accessory proteins. xrRNAs halt the enzyme at a defined location such that the viral RNA located downstream of the xrRNAs is protected from degradation. The xrRNAs from Zika virus or Murray Valley encephalitis virus, for example, comprise three-way junction and multiple pseudoknot interactions that create an unusual and complex fold that requires a set of nucleotides conserved across the MBFVs structure. xrRNAs halt the enzyme at a defined location such that the viral RNA located downstream of the xrRNAs is protected from degradation. The 5′-end of the RNA passes through a ring-like structure of the fold and is believed to remain protected from the Xrn1-like exonuclease. 
     In some embodiments, the nucleic acid construct comprising the one or more sequences that encode a protein of interest may comprise one or more xrRNA structures incorporated therein. In some embodiments, the xrRNA is a stretch of nucleotides having the conserved regions of the 3′ UTR of one or more viral xrRNA sequences. In some embodiments, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more xrRNA elements are incorporated within the nucleic acid construct. In some embodiments, 2 or more xrRNA elements are incorporated in tandem within the nucleic acid construct. In some embodiments, the xrRNA comprise one or more regions comprising conserved sequences or fragments thereof or modifications thereof. In some embodiments, the xrRNA is placed at the 3′UTR of a retrotransposon element. In some embodiments, the xrRNA is placed at upstream of the sequences encoding the one or more proteins or polypeptides. In some embodiments, the xrRNA is placed in the 3′UTR of a retrotransposon element, such as an ORF2 sequence, and upstream of the sequences encoding the one or more proteins or polypeptides. 
     In some embodiments, the xrRNA structure comprises a MBFV xrRNA sequence, or a sequence that is at least 90% identical thereof. In some embodiments, the xrRNA structure comprises a tick-borne flaviviruses (TBFVs) xrRNA sequence, or a sequence that is at least 90% identical thereof. In some embodiments, the xrRNA structure comprises a tick-borne flaviviruses (TBFVs) xrRNA sequence, or a sequence that is at least 90% identical thereof. In some embodiments, the xrRNA structure comprises a tick-borne flaviviruses (TBFVs) xrRNA sequence, or a sequence that is at least 90% identical thereof. In some embodiments, the xrRNA structure comprises a xrRNA sequence from a member of no known arthropod vector flaviviruses (NKVFVs), or a sequence that is at least 90% identical thereof. In some embodiments, the xrRNA structure comprises a xrRNA sequence from a member of insect-specific flaviviruses (ISFVs), or a sequence that is at least 90% identical thereof. In some embodiments, the xrRNA structure comprises a Zikavirus xrRNA sequence, or a sequence that is at least 90% identical thereof. It is hereby contemplated that any known xrRNA structural elements or conceivable non-obvious variations thereof may be used for the purpose described herein. 
     Several messenger RNAs from different organisms exhibit one or more pseudoknot structures that exhibits resistance from 5′-3′ exonuclease. A pseudoknot is a RNA structure that is minimally composed of two helical segments connected by single-stranded regions or loops. Although several distinct folding topologies of pseudoknots exist. 
     Poly A Tail Modifications 
     The poly A structure in the 3′UTR of an mRNA is an important regulator of mRNA half-life. Deadenylation of the 3′ end of the poly A tail is the first step of the intracellular mRNA degradation. In some embodiments, the length of the poly A tail of the mRNA construct is taken into critical consideration and designed for maximizing the expression of the protein encoded by the mRNA coding region, and the mRNA stability. In some embodiments, the nucleic acid construct comprises one or more poly A sequences. In some embodiments, the poly A sequence at the 3′UTR of the sequences encoding the one or more proteins or polypeptides comprise 20-200 adenosine nucleobases. In some embodiments, the poly A sequence comprises 30-200 adenosine nucleobases. In some embodiments, the poly A sequence comprises 50-200 adenosine nucleobases. In some embodiments, the poly A sequence comprises 80-200 adenosine nucleobases. In some embodiments, the mRNA segment comprising the sequences that encode one or more proteins or polypeptides comprises a 3′-UTR having a poly-A tail comprising about 180 adenosine nucleobases, or about 140 adenosine nucleobases, or about 120 adenosine nucleobases. In some embodiments, the poly A tail comprises about 122 adenosine nucleobases. In some embodiments, the poly A sequence comprises 50 adenosine nucleobases. In some embodiments, the poly A sequence comprises 30 adenosine nucleobases. In some embodiments, the adenosine nucleobases in the poly A tail are placed in tandem, with or without intervening non-adenosine bases. In some embodiments, one or more non-adenosine nucleobases are incorporated in the poly A tail, which confer further resistance to certain exonucleases. 
     In some embodiments, the stretch of adenosines in poly A tail of the construct comprises one or more non-adenosine (A) nucleobase. In some embodiments, the non-A nucleobase is present at −3, −2, −1, and/or +1 position at the poly A 3′-terminal region. In some embodiments, the non-A bases comprise a guanosine (G) or a cytosine (C) or an uracil base (U). In some embodiments, the non-A base is a G. In some embodiments, the non-A base more than one, in tandem, for example, GG. In some embodiments, the modification at the 3′ end of the poly A tail with one or more non-A base is directed at disrupting the A base stacking at the poly A tail. The poly A base stacking promotes deadenylation by various deadenylating enzymes, and therefore 3′ end of poly A tail ending in -AAAG, -AAAGA, or -AAAGGA are effective in conferring stability against deadenylation. In some organisms, a GC sequence intervening a poly A sequence is shown to effectively show down 3′-5′ exonuclease mediated decay. A modification contemplated herein comprises an intervening non-A residue, or a non-A residue duplex intervening a poly A stretch at the 3′ end. 
     In some embodiments, a triplex structure is introduced in the 3′ UTR which effectively stalls or slows down exonuclease activity involving the 3′ end. 
     In some embodiments, the mRNA with the modifications described above has an extended half-life and demonstrates stable expression over a longer period than the unmodified mRNA. In some embodiments, the mRNA stably expresses for greater than 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 8 days 9 days or 10 days or more, and the mRNA or its protein product is detectable in vivo. In some embodiments, the mRNA is detected up to 3 days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days or 15 days in vivo. In some embodiments, a protein product of the mRNA is detected up to 3 days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days, 15 days, 16 days, 17 days, 18 days, 20 days, 25 days or 30 days in vivo. 
     CircRNA and tectoRNA 
     Circular RNA is useful in the design and production of stable form of RNA used as a messenger RNA to direct synthesis protein chains, such as long, multiply repeating protein chains. There are few methods to make circular RNA (circRNA). They include protein-mediated ligation of RNA ends using RNA ligase and using a split self-splicing intron, such that if the two halves of the intron are located and the ends of a transcribed mRNA, the intron will splice itself out and leave a ligated product ( FIG.  3 A ). Another technique relies on the ability of T4 DNA ligase to act as an RNA ligase when the RNA ends to be ligated are held together by an oligonucleotide. Both these techniques suffer from inefficiency and require a large amount of enzyme. A third technique uses the cyclization or circularization activity of group I introns where most of the intron sequences that carry out the reaction must remain a part of the circle. Group I introns share a complex set of secondary and tertiary structures containing a series of conserved RNA stem loops which form the catalytic core. Many of these introns are self-splicing in vitro and can splice and form two ligated exons as RNA with no accessory protein factors. The products created by the group I autocatalytic reaction are (1) an upstream exon ligated at the 5′ splice site to the 3′ splice site of a downstream exon and (2) a linear intron that can undergo further reversible auto-catalysis to form a circular intron. The presence of such a large highly structured nucleic acid sequence severely limits the types of RNA sequences that can be made circular by that technique. In addition, the catalytic activity of the intron may remain and interfere with structure and function of the circular RNA. 
     It is useful to increase the rate of the reaction, and thus the overall efficiency, by bringing the ends of the RNA in closer proximity. Previous work has achieved this by including complementary RNA sequences 3′ and 5′ to the ends of the mRNA such that upon hybridization of these sequences, the ends of the mRNA are in closer proximity such that it can undergo the ligation or self-splicing reaction with an overall faster rate compared to without the complementary sequences. These are called homology arms ( FIG.  3 A ) of the self-splicing version of the circularization reaction. A major issue with such hybridization strategy is that if there are complementary sequences within the coding region to either of the homology arms, hybridization would actually inhibit the splicing reaction and the arms would need to be optimized for each new coding region. An alternative to this strategy described herein is the use of RNA sequences that fold a three-dimensional structure to form a stable binding interaction that is independent of sequence. 
     Non-Watson-Crick RNA tertiary interactions can be exploited to construct ‘tectoRNA’ molecular units, defined as RNA molecules capable of self-assembly. The use of such type of tertiary interactions allows one to control and modulate the assembly process by manipulating cation concentration (e.g. Mg 2+ ), and/or suitable temperature and employing modularly designed ‘selector’ RNA molecules. For the self-assembly of one-dimensional arrays, a basic modular unit was designed that comprises a 4-way junction with an interacting module on each helical arm. In some embodiments, the interacting module is a GAAA loop or a specific GAAA loop receptor. Each tectoRNA can interact with two other tectoRNAs via the formation of four loop-receptor interactions, two with each partner molecule. 
     In some embodiments, the tectoRNA structures are suitably selected, and integrated in the RNA comprising the exon and intron to form a circRNA. In some embodiments, the integration is done by well-known molecular biology techniques such as ligation. In some embodiments, the tectoRNA forms a stable structure at high temperatures. The tectoRNA structure do not compete with internal RNA sequences, thereby creating high efficiency circularization and splicing. 
     The circRNA can comprise a coding sequence described in any of the preceding sections. For example, it can comprise a sequence encoding fusion protein comprising a tethering or a receptor molecule. The receptor can be a phagocytic receptor fusion protein. 
     In some embodiments, the intron is a self-splicing intron. 
     In some embodiments, the terminal regions having the tertiary structures, also termed scaffolding regions for the circRNA, are about 30 nucleotides to about 100 nucleotides long. In some embodiments, the tertiary structure motif is about 45 nucleotides, about 50 nucleotides, about 55 nucleotides, about 60 nucleotides, about 65 nucleotides, about 70 nucleotides or about 75 nucleotides long. In some embodiments, the tertiary motifs are formed at high temperatures. In some embodiments, the tertiary motifs are stable. 
     In some embodiments, the nucleic acid construct having the one or more modifications as described herein and comprising one or more sequences encoding one or more proteins or polypeptides, is stable when administered in vivo. In some embodiments, the nucleic acid is an mRNA. In some embodiments, the mRNA comprising one or more sequences encoding one or more proteins or polypeptides is stable in vivo for more than 2 days, for more than 3 days, more than 4 days, more than 5 days, more than 6 days, more than 7 days, more than 8 days, more than 9 days, more than 10 days, more than 11 days, more than 12 days, more than 13 days, more than 14 days, more than 15 days, more than 16 days, more than 17 days, more than 18 days, more than 19 days, or more than 20 days. In some embodiments, the protein encoded by the sequences in the mRNA can be detected in vivo at greater than 3 days, 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days, 15 days, 16 days, 17 days, 18 days, 19 days, or 20 days. In some embodiments, the protein encoded by the sequences in the mRNA can be detected in vivo for about 7 days after the mRNA is administered. In some embodiments, the protein encoded by the sequences in the mRNA can be detected in vivo for about 14 days after the mRNA is administered. In some embodiments, the protein encoded by the sequences in the mRNA can be detected in vivo for about 21 days after the mRNA is administered. In some embodiments, the protein encoded by the sequences in the mRNA can be detected in vivo for about 30 days after the mRNA is administered. In some embodiments, the protein encoded by the sequences in the mRNA can be detected in vivo for more than about 30 days after the mRNA is administered. 
     In some aspects, enhancing nucleic acid uptake or incorporation within the cell is contemplated for enhancing expression of the retrotransposition. One of the methods include obtaining a homogenous population of cells to initiate incorporation of the nucleic acid, e.g. via transfection, in case of plasmid vector constructs, or via electroporation or any other means that may be used suitably to deliver a nucleic acid molecule into the cell. In some embodiments, cell cycle synchronization may be sought. Cell cycle synchronization may be accomplished by sorting cells for a certain common phenotype. In some embodiments, the cell population may be subjected to a treatment with a reagent that can stall cell cycle progression of all cells at a certain stage. Exemplary reagents can be found in commercial databases, such as www.tocris.com/cell-biology/cell-cycle-inhibitors, or www.scbt.com/browse/chemicals-Other-Chemicals-cell-cycle-arresting-compounds. For example, itraconazole or nocodazole inhibits cell cycle at G1 phase, or reagents that arrest cell cycle at G0/G1 phase, for example, 5-[(4-Ethylphenyl)methylene]-2-thioxo-4-thiazolidinone (compound 10058-F4) (Tocris Bioscience); or a G2M cell cycle blocker, such as AZD 5438 (chemical name, 4-[2-Methyl-1-(1-methylethyl)-1H-imidazol-5-yl]-N-[4-(methylsulfonyl)phenyl]-2-pyrimidinamine) which blocks cell cycle at G2M, G1 or S phases, to name a few. Cyclosporin, hydroxyurea, thymidine, are well known reagents that can cause cell cycle arrests. Some reagents may irreversibly alter a cell state or may be toxic for the cells. Serum deprivation of cells for about 2-16 hours prior to electroporation or transfection, depending on the cell type, may also be an easy and reversible strategy for cell synchronization. 
     In some embodiments, retrotransposition efficiency may be increased by encouraging generation of DNA double stranded breaks to a cell that has been transfected with or electroporated with the retrotransposition constructs as described herein and/or modulating the DNA repair machinery. 
     Application of these techniques may be limited depending on end uses of the cell that would undergo the genetic manipulation ex vivo for stable incorporation of a nucleic acid sequence by this method. In some cases, use of such techniques may be contemplated where robust expression of the protein or transcript encoded by the incorporated nucleic acid is expected as an outcome for a determined period of time. Method of introducing double stranded breaks in a cell include subjecting the cell to controlled ionizing radiation of about 0.1 Gy or less for a short period. 
     In some embodiments, efficiency of LINE-1 mediated retrotransposition may be increased by treating the cell with small molecule inhibitors of DNA repair proteins to increase the window for the reverse transcriptase to act. Exemplary small molecule inhibitors of DNA repair proteins may be Benzamide (CAS 55-21-0), Olaparib (Lynparza) (CAS 763113-22-0), Rucaparib (Clovis -AG014699, PF-01367338 Pfizer), Niraparib (MK-827 Tesaro) CAS 1038915-60-4); Veliparib (ABT-888 Abbvie) (CAS 912444-00-9); Camptothecin (CPT) (CAS 7689-03-4); Irinotecan (CAS 100286-90-6); Topotecan (Hycamtin® GlaxoSmithKline) (CAS 123948-87-8); NSC 19630 (CAS 72835-26-8); NSC 617145 (CAS 203115-63-3); ML216 (CAS 1430213-30-1); 6-hydroxyDL-dopa (CAS 21373-30-8); D-103; D-G23; DIDS (CAS 67483-13-0); B02 (CAS 1290541-46-6); RI-1 (CAS 415713-60-9); RI-2 (CAS 1417162-36-7); Streptonigrin (SN) (CAS 3930-19-6). 
     III. Nucleic Acid Carzo: 
     A. Transgene 
     In one aspect the transgene or noncoding sequence that is the heterologous nucleic acid sequence to be inserted within the genome of a cell is delivered as an mRNA. The mRNA may comprise greater than about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10,000 bases. In some embodiments, the mRNA may be more than 10,000 bases long. In some embodiments, the mRNA may be about 11,000 bases long. 
     In some embodiments, the mRNA may be about 12,000 bases long. In some embodiments, the mRNA comprises a transgene sequence that encodes a fusion protein. In some embodiments, the nucleic acid is delivered as a plasmid. 
     In some embodiments, the nucleic acid is delivered in the cell by transfection. In some embodiments, the nucleic acid is delivered in the cell by electroporation. In some embodiments, the transfection or electroporation is repeated more than once to enhance incorporation of the nucleic acid into the cell. 
     Contemplated herein are retrotransposon mediated stable integration of a recombinant nucleic acid encoding a phagocytic or tethering receptor (PR) fusion protein (CFP). In some embodiments, the CFPs comprise: a PR subunit comprising: a transmembrane domain, and an intracellular domain comprising an intracellular signaling domain; and an extracellular domain comprising an antigen binding domain specific to an antigen of a target cell; wherein the transmembrane domain and the extracellular domain are operatively linked. 
     In some embodiments, the nucleic acid comprises a sequence encoding a chimeric fusion protein (CFP), the CFP comprising an extracellular domain comprising a CD5 binding domain, and a transmembrane domain operatively linked to the extracellular domain. In some embodiments, the CD5 binding domain is a CD5 binding protein, such as an antigen binding fragment of an antibody, a Fab fragment, an scFv domain or an sdAb domain. In some embodiments, wherein the CD5 binding domain comprises an scFv comprising (i) a variable heavy chain (VH) sequence with at least 90% sequence identity to EIQLVQSGGGLVKPGGSVRISCAASGYTFTNYGMNWVRQAPGKGLEWMGWINTHTGEPTYAD SFKGRFTFSLDDSKNTAYLQINSLRAEDTAVYFCTRRGYDWYFDVWGQGTTVTV (SEQ ID NO: 1); and (ii) a variable light chain (VL) sequence with at least 90% sequence identity to DIQMTQSPSSLSASVGDRVTITCRASQDINSYLSWFQQKPGKAPKTLIYRANRLESGVPSRFSGSG SGTDYTLTISSLQYEDFGIYYCQQYDESPWTFGGGTKLEIK (SEQ ID NO: 2). In some embodiments, the CFP further comprises an intracellular domain, wherein the intracellular domain comprises one or more intracellular signaling domains, and wherein a wild-type protein comprising the intracellular domain does not comprise the extracellular domain. In some embodiments, the one or more intracellular signaling domains comprises a phagocytic signaling domain. In some embodiments, the phagocytosis signaling domain comprises an intracellular signaling domain derived from a receptor other than Megf10, MerTk, FcαR, and Bai1. In some embodiments, the phagocytosis signaling domain comprises an intracellular signaling domain derived from FcγR, FcαR or FcεR. In some embodiments, the phagocytosis signaling domain comprises an intracellular signaling domain with at least 90% sequence identity to LYCRRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYETLKHEKPP (SEQ ID NO: 67). In some embodiments, the one or more intracellular signaling domains further comprises a proinflammatory signaling domain. In some embodiments, the proinflammatory signaling domain comprises a PI3-kinase (PI3K) recruitment domain. In some embodiments, the proinflammatory signaling domain comprises a sequence with at least 90% sequence identity to YEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENM (SEQ ID NO: 4). In some embodiments, the proinflammatory signaling domain is derived from an intracellular signaling domain of CD40. In some embodiments, the proinflammatory signaling domain comprises a sequence with at least 90% sequence identity to KVAKKPTNKAPHPKQEPQEINFPDDLPGSNTAAPVQETLHGCQPVTQEDGKESRISVQERQ (SEQ ID NO: 68). In some embodiments, the transmembrane domain comprises a CD8 transmembrane domain. In some embodiments, the transmembrane domain comprises a sequence with at least 90% sequence identity to IYIWAPLAGTCGVLLLSLVIT (SEQ ID NO: 6). In some embodiments, the extracellular domain further comprises a hinge domain derived from CD8, wherein the hinge domain is operatively linked to the transmembrane domain and the CD5 binding domain. In some embodiments, the extracellular domain comprises a sequence with at least 90% sequence identity to ALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLD (SEQ ID NO: 7). In some embodiments, the CFP comprises an extracellular domain comprising a scFv that specifically binds CD5, and a hinge domain derived from CD8; a hinge domain derived from CD28 or at least a portion of an extracellular domain from CD68; a CD8 transmembrane domain, a CD28 transmembrane domain or a CD68 transmembrane domain; and an intracellular domain comprising at least two intracellular signaling domains, wherein the at least two intracellular signaling domains comprise: a first intracellular signaling domain derived from FcγR or FcεR, and a second intracellular signaling domain comprising a PI3K recruitment domain, or derived from CD40. In some embodiments, the recombinant polynucleic acid is an mRNA or circRNA. In some embodiments, the nucleic acid is delivered into a myeloid cell. In some embodiments, the nucleic acid is delivered into a CD14+ cell, a CD14+CD16− cell, an M0 macrophage, an M2 macrophage, an M1 macrophage or a mosaic myeloid cell/macrophage. In some embodiments, the fusion protein comprises a sequence with at least 90% sequence identity to EIQLVQSGGGLVKPGGSVRISCAASGYTFTNYGMNWVRQAPGKGLEWMGWINTHTGEPTYAD SFKGRFTFSLDDSKNTAYLQINSLRAEDTAVYFCTRRGYDWYFDVWGQGTTVTVSSGGGGSGG GGSGGGGSDIQMTQSPSSLSASVGDRVTITCRASQDINSYLSWFQQKPGKAPKTLIYRANRLESG VPSRFSGSGSGTDYTLTISSLQYEDFGIYYCQQYDESPWTFGGGTKLEIKSGGGGSGALSNSIMYF SHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDIYIWAPLAGTCGV LLLSLVITLYCRRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYETLKHEKPPQGSGSYEDMRGI LYAAPQLRSIRGQPGPNHEEDADSYENM (SEQ ID NO: 69). In some embodiments, the fusion protein comprises a sequence with at least 90% sequence identity to 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 70) 
               
               
                   
                 EIQLVQSGGGLVKPGGSVRISCAASGYTFTNYGMN 
               
               
                   
                   
               
               
                   
                 WVRQAPGKGLEWMGWINTHTGEPTYADSFKGRFTF 
               
               
                   
                   
               
               
                   
                 SLDDSKNTAYLQINSLRAEDTAVYFCTRRGYDWYF 
               
               
                   
                   
               
               
                   
                 DVWGQGTTVTVSSGGGGSGGGGSGGGGSDIQMTQS 
               
               
                   
                   
               
               
                   
                 PSSLSASVGDRVTITCRASQDINSYLSWFQQKPGK 
               
               
                   
                   
               
               
                   
                 APKTLIYRANRLESGVPSRFSGSGSGTDYTLTISS 
               
               
                   
                   
               
               
                   
                 LQYEDFGIYYCQQYDESPWTFGGGTKLEIKSGGGG 
               
               
                   
                   
               
               
                   
                 SGALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPA 
               
               
                   
                   
               
               
                   
                 PTIASQPLSLRPEACRPAAGGAVHTRGLDIYIWAP 
               
               
                   
                   
               
               
                   
                 LAGTCGVLLLSLVITLYCRLKIQVRKAAITSYEKS 
               
               
                   
                   
               
               
                   
                 DGVYTGLSTRNQETYETLKHEKPPQKKVAKKPTNK 
               
               
                   
                   
               
               
                   
                 APHPKQEPQEINFPDDLPGSNTAAPVQETLHGCQP 
               
               
                   
                   
               
               
                   
                 VTQEDGKESRISVQERQ 
               
               
                   
                 or 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 71) 
               
               
                   
                 EIQLVQSGGGLVKPGGSVRISCAASGYTFTNYGMN 
               
               
                   
                   
               
               
                   
                 WVRQAPGKGLEWMGWINTHTGEPTYADSFKGRFTF 
               
               
                   
                   
               
               
                   
                 SLDDSKNTAYLQINSLRAEDTAVYFCTRRGYDWYF 
               
               
                   
                   
               
               
                   
                 DVWGQGTTVTVSSGGGGSGGGGSGGGGSDIQMTQS 
               
               
                   
                   
               
               
                   
                 PSSLSASVGDRVTITCRASQDINSYLSWFQQKPGK 
               
               
                   
                   
               
               
                   
                 APKTLIYRANRLESGVPSRFSGSGSGTDYTLTISS 
               
               
                   
                   
               
               
                   
                 LQYEDFGIYYCQQYDESPWTFGGGTKLEIKSGGGG 
               
               
                   
                   
               
               
                   
                 SGALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPA 
               
               
                   
                   
               
               
                   
                 PTIASQPLSLRPEACRPAAGGAVHTRGLDIYIWAP 
               
               
                   
                   
               
               
                   
                 LAGTCGVLLLSLVITLYCRRLKIQVRKAAITSYEK 
               
               
                   
                   
               
               
                   
                 SDGVYTGLSTRNQETYETLKHEKPPQKKVAKKPTN 
               
               
                   
                   
               
               
                   
                 KAPHPKQEPQEINFPDDLPGSNTAAPVQETLHGCQ 
               
               
                   
                   
               
               
                   
                 PVTQEDGKESRISVQERQ. 
               
            
           
         
       
     
     In some embodiments, the fusion protein is a transmembrane protein, an intracellular protein or an intracellular protein. In one embodiment the fusion protein is directed to enhancing the function of an immune cell, e.g., a myeloid cell, selected from monocyte, macrophages dendritic cells or precursors thereof. In one embodiment the fusion protein augments a cellular function of an immune cell, such as phagocytosis. The disclosure is not limited by the transgenes that can be expressed using the methods and compositions described. The transgenes indicated in this section are exemplary. 
     Provided herein are exemplary transgene candidates, for stable integration into the genome of a phagocytic cell. In one embodiment the transgene is a recombinant nucleic acid encoding a phagocytic receptor (PR) fusion protein (CFP). The recombinant nucleic acid has a PR subunit comprising: (i) a transmembrane domain, and (ii) an intracellular domain comprising a phagocytic receptor intracellular signaling domain; and an extracellular antigen binding domain specific to an antigen of a target cell; wherein the transmembrane domain and the extracellular antigen binding domain are operatively linked such that antigen binding to the target by the extracellular antigen binding domain of the fused receptor activated in the intracellular signaling domain of the phagocytic receptor. In some embodiments, the recombinant nucleic acid encodes a chimeric antigen receptor. In some embodiments, the chimeric antigen receptor is a chimeric antigen receptor (phagocytosis) (CAR-P). In some embodiments, the fusion protein is a recombinant protein for locking anti-phagocytic signals. In some embodiments, the fusion protein is a phagocytosis enhancing chimeric protein. In some embodiments, the chimeric protein has intracellular domains comprising active phagocytosis signal transduction domains. In some embodiments, the chimeric protein enhances the phagocytic potential by enhancing the inflammatory potential of the phagocytic cell in which it expresses. In some embodiments, the transgene is designed to express a chimeric protein which is activated by contact with an antigen in a target cell, whereupon the phagocytic cell phagocytoses the target cell and kills the target cell. 
     The terms “spacer” or “linker” as used in reference to a fusion protein refers to a peptide sequence that joins the protein domains of a fusion protein. Generally, a spacer has no specific biological activity other than to join or to preserve some minimum distance or other spatial relationship between the proteins or RNA sequences. However, in some embodiments, the constituent amino acids of a spacer can be selected to influence some property of the molecule such as the folding, net charge, or hydrophobicity of the molecule. Suitable linkers for use in an embodiment of the present disclosure are well known to those of skill in the art and include, but are not limited to, straight or branched-chain carbon linkers, heterocyclic carbon linkers, or peptide linkers. The linker is used to separate two antigenic peptides by a distance sufficient to ensure that, in some embodiments, each antigenic peptide properly folds. Exemplary peptide linker sequences adopt a flexible extended conformation and do not exhibit a propensity for developing an ordered secondary structure. Typical amino acids in flexible protein regions include Gly, Asn and Ser. Virtually any permutation of amino acid sequences containing Gly, Asn and Ser would be expected to satisfy the above criteria for a linker sequence. Other near neutral amino acids, such as Thr and Ala, also can be used in the linker sequence. 
     The various exemplary proteins encoded by a transgene that can be expressed for enhancing the immune potential of a phagocytic cell are described below. This is not an exhaustive list but serves as an exemplary list for transgene design within the scope of the present disclosure. 
     In some embodiments, the PSP subunit comprises a transmembrane (TM) domain of a phagocytic receptor. 
     In some embodiments, the PSP subunit comprises an ICD domain of a phagocytic receptor. 
     In some embodiments, the ICD encoded by the recombinant nucleic acid comprises a domain selected from the group consisting of lectin, dectin 1, mannose receptor (CD206), scavenger receptor A1 (SRA1), MARCO, CD36, CD163, MSR1, SCARA3, COLEC12, SCARA5, SCARB1, SCARB2, CD68, OLR1, SCARF1, SCARF2, CXCL16, STAB1, STAB2, SRCRB4D, SSC5D, CD205, CD207, CD209, RAGE, CD14, CD64, F4/80, CCR2, CX3CR1, CSF1R, Tie2, HuCRIg(L), and CD169 receptor. 
     In some embodiments, the ICD comprises the signaling domain derived from any one or more of: lectin, dectin 1, mannose receptor (CD206), scavenger receptor A1 (SRA1), MARCO (Macrophage Receptor with Collagenous Structure, aliases: SRA6, SCARA2), CD36 (Thrombospondin receptor, aliases: Scavenger Receptor class B, member 3), CD163 (Scavenger receptor, cysteine rich-type 1), MSR1, SCARA3, COLEC12 (aliases: Scavenger Receptor With C-Type Lectin, SCARA4, or Collectin 12), SCARA5, SCARB1, SCARB2, CD68 (SCARD, microsialin), OLR1 (Oxidized Low Density Lipoprotein Receptor 1, LOX1, or C-Type Lectin Domain Family 8 Member A), SCARF1, SCARF2, SRCRB4D, SSC5D, and CD169 (aliases, Sialoadhesin receptor, SIGLEC1). 
     In some embodiments, the recombinant nucleic acid encodes, for example, an intracellular domain of human MARCO. The PSR subunit comprises an intracellular domain having a 44 amino acid ICD of human MARCO having an amino acid sequence: MRNKKILKEDELLSETQQAAFHQIAMEPFEINVPKPKRRNGVNF (SEQ ID NO: 72). In some embodiments, the PSR subunit comprises a variant which is at least 70%, 75%, 80%, 85%, 90% or 95% identical to the intracellular domain of MARCO. 
     In some embodiments, for example, the PSR (phagocytic scavenger receptor) comprises a transmembrane region of human MARCO. 
     In some embodiments, the recombinant nucleic acid encodes an intracellular domain of human SRA1. The PSR subunit comprises an intracellular domain having a 50 amino acid ICD of human SRA1 having an amino acid sequence: MEQWDHFHNQQEDTDSCSESVKFDARSMTA LLPPNPKNSPSLQEKLKSFK (SEQ ID NO: 73). In some embodiments, the PSR subunit comprises a variant which is at least 70%, 75%, 80%, 85%, 90% or 95% identical to the intracellular domain of human SRA1. The intracellular region of SRA has a phosphorylation site. 
     In some embodiments, the PSR comprises a transmembrane region of human SRA1. 
     In some embodiments, for example, the recombinant nucleic acid comprises an intracellular domain of CD36. In some embodiments, the recombinant nucleic acid comprises a TM domain of CD36. Naturally occurring full length CD36 has two TM domains and two short intracellular domains, and an extracellular domain of CD36 binds to oxidized LDL. Both of the intracellular domains contain pairs of cysteines that are fatty acid acylated. It lacks known signaling domains (e.g. kinase, phosphatase, g-protein binding, or scaffolding domains). N-terminal cytoplasmic domain is extremely short (5-7 amino acid residues) and is closely associated with the internal leaflet of the plasma membrane. The carboxy-terminal domain contains 13 amino acids, containing a CXCX5K motif homologous to a region in the intracellular domain of CD4 and CD8 that is known to interact with signaling molecules. The intracellular domain of CD36 is capable of assembling a signaling complex that activates lyn kinases, MAP kinases and Focal Adhesion Kinases (FAK), and inactivation of src homology 2-containing phosphotyrosine phosphatase (SHP-2). Members of the guanine nucleotide exchange factors (GEFs) have been identified as potential key signaling intermediates. 
     In some embodiments, the recombinant nucleic acid encodes for example, an intracellular domain of human SCARA3. In some embodiments, the PSR subunit comprises a variant which is at least 70%, 75%, 80%, 85%, 90% or 95% identical to the intracellular domain of human SCARA3. In some embodiments, the PSR comprises the TM domain of SCARA3. In some embodiments, the TM domains are about 20-30 amino acids long. 
     Scavenger receptors may occur as homo or hetero dimers. MARCO, for example occurs as a homo trimer. 
     In some embodiments, the TM domain or the ICD domain of the PSP is not derived from FcR, Megf10, Bai1 or MerTK. In some embodiments, the ICD of the PSR does not comprise a CD3 zeta intracellular domain. 
     In some embodiments, the intracellular domain and transmembrane domains are derived from FcR beta. 
     In one aspect the recombinant nucleic acid encodes a chimeric antigenic receptor for enhanced phagocytosis (CAR-P), which is a phagocytic scavenger receptor (PSR) fusion protein (CFP) comprising: (a) an extracellular domain comprising an extracellular antigen binding domain specific to an antigen of a target cell, (b) a transmembrane domain, and (c) a recombinant PSR intracellular signaling domain, wherein the recombinant PSR intracellular signaling domain comprises a first portion derived from a phagocytic and a second portion derived from non-phagocytic receptor. 
     In some embodiments, the second portion is not a PI3K recruitment domain. In some embodiments, the second portion is a PI3K recruitment domain. 
     The second portion derived from non-phagocytic receptor may comprise an intracellular signaling domain that enhances phagocytosis, and/or inflammatory potential of the engineered phagocytic cells expressing the recombinant nucleic acid. In some embodiments, the second portion derived from non-phagocytic receptor comprises more than one intracellular domain (ICD). In some embodiments, the second portion derived from non-phagocytic receptor comprises a second ICD. In some embodiments, the second portion derived from non-phagocytic receptor comprises a second and a third ICD. In some embodiments, the second portion derived from non-phagocytic receptor comprises a second, a third and a fourth ICD, wherein the second portion is encoded by the recombinant nucleic acid. The respective second portions comprising a second, or third or fourth ICD derived from non-phagocytic receptor are described as follows. 
     Chimeric Antigen Receptors for Enhancing Intracellular Signaling and Inflammation Activation 
     In one aspect, the recombinant nucleic acid encodes a second intracellular domain in addition to the phagocytic ICD, which confers capability of potent pro-inflammatory immune activation, such as when macrophages engage in fighting infection. The second intracellular domain (second ICD) is fused to the cytoplasmic terminus of the first phagocytic ICD. The second intracellular domain provides a second signal is necessary to trigger inflammasomes and pro-inflammatory signals. Nod-like receptors (NLRs) are a subset of receptors that are activated in innate immune response, and oligomerize to form multi-protein complexes that serve as platforms to recruit proinflammatory caspases and induce their cleavage and activation. This leads to direct activation of ROS, and often result in a violent cell death known as pyroptosis. There are four inflammasome complexes, NLRP1m, NLRP3, IPAF and AIM2. 
     The tumor microenvironment (TME) constitutes an immunosuppressive environment. Influence of IL-10, glucocorticoid hormones, apoptotic cells, and immune complexes can interfere with innate immune cell function. Immune cells, including phagocytic cells settle into a tolerogenic phenotype. In macrophages, this phenotype, commonly known as the M2 phenotype is distinct from the M1 phenotype, where the macrophages are potent and capable of killing pathogens. Macrophages exposed to LPS or IFN-gamma, for example, can polarize towards an M1 phenotype, whereas macrophages exposed to IL-4 or IL-13 will polarize towards an M2 phenotype. LPS or IFN-gamma can interact with Toll-like receptor 4 (TLR4) on the surface of macrophages inducing the Trif and MyD88 pathways, inducing the activation of transcription factors IRF3, AP-1, and NFKB and thus activating TNFs genes, interferon genes, CXCL10, NOS2, IL-12, etc., which are necessary in a pro-inflammatory M1 macrophage response. Similarly, IL-4 and IL-13 bind to IL-4R, activation the Jak/Stat6 pathway, which regulates the expression of CCL17, ARG1, IRF4, IL-10, SOCS3, etc., which are genes associated with an anti-inflammatory response (M2 response). Expression of CD14, CD80, D206 and low expression of CD163 are indicators of macrophage polarization towards the M1 phenotype. 
     In some embodiments, the recombinant nucleic acid encodes one or more additional intracellular domains, comprising a cytoplasmic domain for inflammatory response. In some embodiments, expression of the recombinant nucleic acid encoding the phagocytic receptor (PR) fusion protein (CFP) comprising the cytoplasmic domain for inflammatory response in the engineered macrophages confers potent pro-inflammatory response similar to the M1 phenotype. 
     In some embodiments, the cytoplasmic domain for inflammatory response can be the signal transducing domains or regions of TLR3, 4, 9, MYD88, TRIF, RIG-1, MDA5, CD40, IFN receptor, NLRP-1-14, NOD1, NOD2, Pyrin, AIM2, NLRC4, CD40. 
     In some embodiments, the expression of the recombinant nucleic acid encoding the phagocytic scavenger receptor (PSR) fusion protein (CFP) comprises a pro-inflammatory cytoplasmic domain for activation of IL-1 signaling cascade. 
     In some embodiments, the cytoplasmic portion of the chimeric receptor (for example, phagocytic receptor (PR) fusion protein (CFP)) comprises a cytoplasmic domain from a toll-like receptor, such as the intracellular signaling domains of toll-like receptor 3 (TLR3), toll-like receptor 4 (TLR4), toll-like receptor 7 (TLR7), toll-like receptor 8 (TLR8), toll-like receptor 9 (TLR9). In some embodiments, the cytoplasmic portion of the chimeric receptor comprises a suitable region from interleukin-1 receptor-associated kinase 1 (IRAK1). In some embodiments, the cytoplasmic portion of the chimeric receptor comprises a suitable region from differentiation primary response protein (MYD88)._In some embodiments, the cytoplasmic portion of the chimeric receptor comprises a suitable region from myelin and lymphocyte protein (MAL). In some embodiments, the cytoplasmic portion of the chimeric receptor comprises a suitable region from retinoic acid inducible gene (RIG-1). 
     In some embodiments, the transmembrane domain of the PSR comprises the transmembrane domain of any one of MYD88, TLR3, TLR4, TLR7, TLR8, TLR9, MAL, IRAK1, proteins. 
     In some embodiments, the recombinant PSR intracellular signaling domain comprises a first portion derived from a phagocytic and a second portion derived from non-phagocytic receptor wherein the second portion derived from non-phagocytic receptor comprises a phosphorylation site. In some embodiments, the phosphorylation site comprises amino acid sequences suitable for an autophosphorylation site. In some embodiments, the phosphorylation site comprises amino acid sequences suitable phosphorylation by Src family kinases. In some embodiments, the phosphorylation site comprises amino acid sequences, which upon phosphorylation are capable of binding to SH2 domains in a kinase. In some embodiments, a receptor tyrosine kinase domain is fused at the cytoplasmic end of the CFP in addition to the first cytoplasmic portion. In some embodiments, the phosphorylation is a tyrosine phosphorylation. 
     In some embodiments, the second intracellular domain is an Immune receptor Tyrosine Activation Motif (ITAM). The ITAM motif is present in mammalian a and 3 immunoglobulin proteins, TCR γ receptors, FCR γ receptors subunits, CD3 chains receptors and NFAT activation molecule. 
     In some embodiments, the CFP intracellular domain comprises one ITAM motif. In some embodiments, the CFP intracellular domain comprises more than one ITAM motifs. In some embodiments, the CFP intracellular domain comprises two or more ITAM motifs. In some embodiments, the CFP intracellular domain comprises three or more ITAM motifs. In some embodiments, the CFP intracellular domain comprises four or more ITAM motifs. In some embodiments, the CFP intracellular domain comprises five or more ITAM motifs. In some embodiments, the CFP intracellular domain comprises six or more ITAM motifs. In some embodiments, the CFP intracellular domain comprises seven or more ITAM motifs. In some embodiments, the CFP intracellular domain comprises eight or more ITAM motifs. In some embodiments, the CFP intracellular domain comprises nine or more ITAM motifs. In some embodiments, the CFP intracellular domain comprises ten or more ITAM motifs. 
     In some embodiments, one or more domains in the first phagocytic ICD comprises a mutation. 
     In some embodiments, one or more domains in the second ICD comprises a mutation to enhance a kinase binding domain, to generate a phosphorylation site, to generate an SH2 docking site or a combination thereof. 
     Co-Expression of an Inflammatory Gene 
     In one aspect, the recombinant nucleic acid comprises a coding sequence for a pro-inflammatory gene, which is co-expressed with the CFP in the engineered cell. In some embodiments, the pro-inflammatory gene is a cytokine. Examples include but not limited to TNF-α, IL-1a, IL-1P, IL-6, CSF, GMCSF, or IL-12 or interferons. 
     The recombinant nucleic acid encoding the proinflammatory gene can be monocistronic, wherein the two coding sequences for (a) the PSP and (b) the proinflammatory gene are post-transcriptionally or post-translationally cleaved for independent expression. 
     In some embodiments, the two coding sequences comprise a self-cleavage domain, encoding a P2A sequence, for example. 
     In some embodiments, the two coding regions are separated by an IRES site. 
     In some embodiments, the two coding sequences are encoded by a bicistronic genetic element. 
     The coding regions for (a) the PSP and (b) the proinflammatory gene can be unidirectional, where each is under a separate regulatory control. In some embodiments, the coding regions for both are bidirectional and drive in opposite directions. Each coding sequence is under a separate regulatory control. 
     Co-expression of the proinflammatory gene is designed to confer strong inflammatory stimulation of the macrophage and activate the surrounding tissue for inflammation. 
     Integrin Activation Domains 
     Cell-cell and cell-substratum adhesion is mediated by the binding of integrin extracellular domains to diverse protein ligands; however, cellular control of these adhesive interactions and their translation into dynamic cellular responses, such as cell spreading or migration, requires the integrin cytoplasmic tails. These short tails bind to intracellular ligands that connect the receptors to signaling pathways and cytoskeletal networks (Calderwood DA, 2004, Integrin Activation, Journal of Cell Science 117, 657-666). Integrins are heterodimeric adhesion receptors formed by the non-covalent association of α and β subunits. Each subunit is a type I transmembrane glycoprotein that has relatively large extracellular domains and, with the exception of the β4 subunit, a short cytoplasmic tail. Individual integrin family members have the ability to recognize multiple ligands. Integrins can bind to a large number of extracellular matrix proteins (bone matrix proteins, collagens, fibronectins, fibrinogen, laminins, thrombospondins, vitronectin, and von Willebrand factor), reflecting the primary function of integrins in cell adhesion to extracellular matrices. Many “counter-receptors” are ligands, reflecting the role of integrins in mediating cell-cell interactions. Integrins undergo conformational changes to increase ligand affinity. 
     The Integrin β 2  subfamily consists of four different integrin receptors, α M β 2  (CD11b/CD18, Mac-1, CR3, Mo-1), α L β 2  (CD11a/CD18, LFA-1), α X β 2  (CD11c/CD18), and α D β 2  (CD11d/CD18). These leukocyte integrins are involved in virtually every aspect of leukocyte function, including the immune response, adhesion to and transmigration through the endothelium, phagocytosis of pathogens, and leukocyte activation. 
     The a subunits of all β 2  integrins contain an inserted region of ˜200 amino acids, termed the I or A domain. Highly conserved I domains are found in several other integrin a subunits and other proteins, such as certain coagulation and complement proteins. I domains mediate protein-protein interactions, and in integrins, they are integrally involved in the binding of protein ligands. Although the I domains dominate the ligand binding functions of their integrins, other regions of the a subunits do influence ligand recognition. As examples, in α M β 2  a mAb (OKM1) recognizing an epitope outside the I domain but in the α M  subunit inhibits ligand binding; and the EF-hand regions in α L β 2  and α 2 β 1 , integrins with I domains in their a subunits, contribute to ligand recognition. The α M  subunit, and perhaps other a subunits, contains a lectin-like domain, which is involved in engagement of non-protein ligands, and occupancy may modulate the function of the I domain. 
     As integrins lack enzymatic activity, signaling is instead induced by the assembly of signaling complexes on the cytoplasmic face of the plasma membrane. Formation of these complexes is achieved in two ways; first, by receptor clustering, which increases the avidity of molecular interactions thereby increasing the on-rate of binding of effector molecules, and second, by induction of conformational changes in receptors that creates or exposes effector binding sites. Within the ECM, integrins have the ability to bind fibronectin, laminins, collagens, tenascin, vitronectin and thrombospondin. Clusters of integrin/ECM interactions form focal adhesions, concentrating cytoskeletal components and signaling molecules within the cell. The cytoplasmic tail of integrins serve as a binding site for α-actinin and talin which then recruit vinculin, a protein involved in anchoring F-actin to the membrane. Talin is activated by kinases such as protein kinase C (PKCα). 
     Integrins are activated by selectins. Leucocytes express L-selectin, activated platelets express P-selectin, and activated endothelial cells express E- and P-selectin. P-selectin-mediated adhesion enables chemokine- or platelet-activating factor-triggered activation of β2 integrins, which stabilizes adhesion. It also facilitates release of chemokines from adherent leucocytes. The cytoplasmic domain of P-selectin glycoprotein ligand 1 formed a constitutive complex with Nef-associated factor 1. After binding of P-selectin, Src kinases phosphorylated Nef-associated factor 1, which recruit the phosphoinositide-3-OH kinase p85-p110 heterodimer and result in activation of leukocyte integrins. E-selectin ligands transduce signals that also affect P2 integrin function. Selectins trigger activation of Src family kinases. SFKs activated by selectin engagement phosphorylate the immunoreceptor tyrosine-based activation motifs (ITAMs) in the cytoplasmic domains of DAP12 and FcRγ. In some respects, CD44 is sufficient to transduce signals from E-selectin. CD44 triggers the inside-out signaling of integrins. A final common step in integrin activation is binding of talin to the cytoplasmic tail of the β subunit. Kindlins, another group of cytoplasmic adaptors, bind to a different region of integrin 3 tails. Kindlins increase the clustering of talin-activated integrins. Kindlins are responsive to selectin signaling, however, kindlins are found mostly in hematopoietic cells, such as neutrophils. Selectin signaling as well as signaling upon integrin activation by chemokines components have shared components, including SFKs, Syk, and SLP-76. 
     In some embodiments, the intracellular domain of the recombinant PSR fusion protein comprises an integrin activation domain. The integrin activation domain comprises an intracellular domain of a selectin, for example, a P-selectin, L-selectin or E-selectin. 
     In some embodiments, the intracellular domain of the recombinant PSR fusion protein comprises an integrin activation domain of laminin. 
     In some embodiments, the intracellular domain of the recombinant PSR fusion protein comprises an integrin activation domain for activation of Talin. 
     In some embodiments, the intracellular domain of the recombinant PSR fusion protein comprises an integrin activation domain fused to the cytoplasmic end of the phagocytic receptor ICD domain. 
     Chimeric Receptor for Enhancing Antigen Cross Presentation 
     In some embodiments, the recombinant nucleic acid encodes a domain capable of enabling cross presentation of antigens. In general, MHC class I molecules present self- or pathogen-derived antigens that are synthesized within the cell, whereas exogenous antigens derived via endocytic uptake are loaded onto MHC class II molecules for presentation to CD4+ T cells. MHC I-restricted presentation of endogenous antigens, in which peptides are generated by the proteasome. However, in some cases, DC can process exogenous antigens into the MHC-1 pathway for presentation to CD8+ T cells. This is referred to as cross presentation of antigens. Soluble or exogenous antigenic components may get degraded by lysosomal proteases in the vacuoles and cross presented by DCs, instead of following the endocytic pathway. In some instances, chaperones, such as heat shock protein 90 (Hsp90) have shown to help cross present antigens by certain APCs. HSP-peptide complexes are known to be internalized by a distinct group of receptors compared to free polypeptides. These receptors are from the scavenger receptor families and included LOX-1, SREC-I/SCARF-I, and FEEL1/Stabilin-1. Both SREC-I and LOX-1 have been shown to mediate the cross presentation of molecular chaperone bound antigens and lead to activation of CD8 +  T lymphocytes. 
     SREC-1 (scavenger receptor expressed by endothelial cells) has no significant homology to other types of scavenger receptors but has unique domain structures. It contains 10 repeats of EGF-like cysteine-rich motifs in the extracellular domain. Recently, the structure of SREC-I was shown to be similar to that of a transmembrane protein with 16 EGF-like repeats encoded by the  Caenorhabditis elegans  gene ced-I, which functions as a cell surface phagocytic receptor that recognizes apoptotic cells. 
     Cross presentation of cancer antigens through the Class-I MHC pathway results in enhanced CD8+ T cell response, which is associated with cytotoxicity and therefore beneficial in tumor regression. 
     In some embodiments, the intracellular domain of the CFP comprises a SREC1 intracellular domain. In some embodiments, the intracellular domain of the CFP comprises a SRECII intracellular domain. 
     In some embodiments, the PSR subunit comprises: an intracellular domain comprising a PSR intracellular signaling domain from SREC1 or SRECII. 
     In some embodiments, the PSR subunit comprises: (i) a transmembrane domain, and (ii) an intracellular domain comprising a PSR intracellular signaling domain from SREC1 or SRECII. 
     In some embodiments, the PSR subunit comprises: (i) a transmembrane domain, (ii) an intracellular domain comprising a PSR intracellular signaling domain, and (iii) an extracellular domain from SREC1 or SRECII. 
     Transmembrane Domain of a CFP Fusion Protein 
     In some embodiments, the TM encoded by the recombinant nucleic acid comprises a domain of a scavenger receptor (SR). In some embodiments, the TM can be the TM domain of or derived from any one or more of: lectin, dectin 1, mannose receptor (CD206), SRA1, MARCO, CD36, CD163, MSR1, SCARA3, COLEC12, SCARA5, SCARB1, SCARB2, CD68, OLR1, SCARF1, SCARF2, SRCRB4D, SSC5D, and CD169. 
     In some embodiments, the TM domains are about 20-30 amino acids long. TM domains of SRs are about 20-30 amino acids long. 
     The TM domain or the ICD domain of the PSP is not derived from Megf10, Bai1 or MerTK. The ICD of the PSR does not comprise a CD3 zeta intracellular domain. 
     In some embodiments, the TM is derived from the same phagocytic receptor as the ICD. 
     In some embodiments, the TM region is derived from a plasma membrane protein. The TM can be selected from an Fc receptor (FcR). In some embodiments, nucleic acid sequence encoding domains from specific FcRs are used for cell-specific expression of a recombinant construct. An FCR-alpha region comprising the TM domain may be used for macrophage specific expression of the construct. FcRβ recombinant protein expresses in mast cells. 
     In some embodiments, the CFP comprises the TM of an FCR-beta (FcRβ). 
     In some embodiments, the CFP comprises both the FcRβTM and ICD domains. 
     In some embodiments, the TM domain is derived from CD8. 
     In some embodiments, the TM is derived from CD2. 
     In some embodiments, the TM is derived from FCR alpha. 
     Extracellular Domain of a CFP Fusion Protein 
     The extracellular domain comprises an antigen binding domain that binds to one or more target antigens on a target cell. The target binding domain is specific for the target. The extracellular domain can include an antibody or an antigen-binding domain selected from intrabodies, peptibodies, nanobodies, single domain antibodies. SMIPs, and multispecific antibodies. 
     In some embodiments, the extracellular domain includes a Fab binding domain. In yet other such embodiments, the extracellular domain includes a scFv. 
     In some embodiments, the chimeric antigen receptor comprises an extracellular antigen binding domain is derived from the group consisting of an antigen-binding fragment (Fab), a single-chain variable fragment (scFv), a nanobody, a VH domain, a VL domain, a single domain antibody (sdAb), a VNAR domain, and a VHH domain, a bispecific antibody, a diabody, or a functional fragment of any thereof. In some embodiments, the antigen-binding fragment (Fab), a single-chain variable fragment (scFv), a nanobody, a VH domain, a VL domain, a single domain antibody (sdAb), a VNAR domain, and a VHH domain, a bispecific antibody, a diabody, or a functional fragment of any thereof specifically bind to one or more antigens. 
     In some embodiments, the antigens are cancer antigens, and the target cell is a target cancer cell. In some embodiments, the antigen for a target cancer cell is selected from the group consisting of CD3, CD4, CD5, CD7, CD19, CCR2, CCR4, CD30, CD37, TCRB1/2, TCR □□, TCR □□. CD22, HER2 (ERBB2/neu), Mesothelin, PSCA, CD123, CD30, CD171, CD138, CS-1, CLECLI, CD33, CD79b, EGFRvIII, GD2, GD3, BCMA, PSMA, RORI, FLT3, TAG72, CD38, CD44v6, CEA, EPCAM, B7H3 (CD276), KIT (CD 117), CD213A2, IL-1 IRa, PRSS21, VEGFR2, CD24, MUC-16, PDGFR-beta, SSEA-4, CD20, MUC1, EGFR, NCAM, Prostase, PAP, ELF2M, Ephrin B2, FAP, EphA2, GM3, TEM1/CD248, TEM7R, CLDN6, TSHR, GPRC5D, CD97, CD179a, ALK, and IGLL1. 
     Various cancer antigen targets can be selected from cancer antigens known to one of skill in the art. Depending on the cancer and the cell type involved cancer antigens are mutated native proteins. The antigen binding domains are screened for specificity towards mutated/cancer antigens and not the native antigens. 
     In some embodiments, for example, the cancer antigen for a target cancer cell can be one or more of the mutated/cancer antigens: MUC16, CCAT2, CTAG1A, CTAG1B, MAGE A1, MAGEA2, MAGEA3, MAGE A4, MAGEA6, PRAME, PCA3, MAGE C1, MAGEC2, MAGED2, AFP, MAGEA8, MAGE9, MAGEA11, MAGEA12, IL13RA2, PLAC1, SDCCAG8, LSP1, CT45A1, CT45A2, CT45A3, CT45A5, CT45A6, CT45A8, CT45A10, CT47A1, CT47A2, CT47A3, CT47A4, CT47A5, CT47A6, CT47A8, CT47A9, CT47A10, CT47A11, CT47A12, CT47B1, SAGE1, and CT55. 
     In some embodiments, for example, the cancer antigen for a target cancer cell can be one or more of the mutated/cancer antigens: CD2, CD3, CD4, CD5, CD7, CD8, CD20, CD30, CD45, CD56, where the cancer is a T cell lymphoma. 
     In some embodiments, for example, the cancer antigen for a target cancer cell can be one or more of the mutated/cancer antigens: IDH1, ATRX, PRL3, or ETBR, where the cancer is a glioblastoma. 
     In some embodiments, for example, the cancer antigen for a target cancer cell can be one or more of the mutated/cancer antigens: CA125, beta-hCG, urinary gonadotropin fragment, AFP, CEA, SCC, inhibin or extradiol, where the cancer is ovarian cancer. 
     In some embodiments, the cancer antigen for a target cancer cell may be HER2. 
     In some embodiments, the cancer antigen for a target cancer cell may be EGFR Variant III. 
     In some embodiments, the cancer antigen for a target cancer cell may be CD19. 
     In some embodiments, the SR subunit region comprises an extracellular domain (ECD) of the scavenger receptor. In some embodiments, the ECD of the scavenger receptor comprises an ECD domain of the SR comprising the ICD and the TM domains. In some embodiments, the SR-ECD contributes to the binding of the phagocyte to the target cell, and in turn is activated, and activates the phagocytosis of the target cell. 
     In some embodiments, the PSR domain optionally comprises the ECD domain or portion thereof of the respective scavenger receptor the ICD and TM domains of which is incorporated in the PSR. 
     Therefore, in some embodiments, In some embodiments, the ECD encoded by the recombinant nucleic acid comprises a domain selected from the group consisting of lectin, dectin 1, mannose receptor (CD206), scavenger receptor A1 (SRA1), MARCO, CD36, CD163, MSR1, SCARA3, COLEC12, SCARA5, SCARB1, SCARB2, CD68, OLR1, SCARF1, SCARF2, CXCL16, STAB1, STAB2, SRCRB4D, SSC5D, CD205, CD207, CD209, RAGE, CD14, CD64, F4/80, CCR2, CX3CR1, CSF1R, Tie2, HuCRIg(L), and CD169 receptor. The extracellular domains of most macrophage scavenger receptors contain scavenger receptors with a broad binding specificity that may be used to discriminate between self and non-self in the nonspecific antibody-independent recognition of foreign substances. The type I and II class A scavenger receptors (SR-All and SR-All) are trimeric membrane glycoproteins with a small NH2-terminal intracellular domain, and an extracellular portion containing a short spacer domain, an α-helical coiled-coil domain, and a triple-helical collagenous domain. The type I receptor additionally contains a cysteine-rich COOH-terminal (SRCR) domain. These receptors are present in macrophages in diverse tissues throughout the body and exhibit an unusually broad ligand binding specificity. They bind a wide variety of polyanions, including chemically modified proteins, such as modified LDL, and they have been implicated in cholesterol deposition during atherogenesis. They may also play a role in cell adhesion processes in macrophage-associated host defense and inflammatory conditions. 
     In some embodiments, the SR ECD is designed to bind to pro-apoptotic cells. In some embodiments, the scavenger receptor ECD comprises a binding domain for a cell surface molecule of a cancer cell or an infected cell. 
     In some embodiments, the extracellular domain of the PR subunit is linked by a linker to a target cell binding domain, such as an antibody or part thereof, specific for a cancer antigen. 
     In some embodiments, the extracellular antigen binding domain comprises one antigen binding domain. In some embodiments, the extracellular antigen binding domain comprises more than one binding domain. In some embodiments, the binding domain is an scFv. In some embodiments, the binding domain is an single domain antibody (sdAb). In some embodiments, the binding domain is fused to the recombinant PR at the extracellular domain. In some embodiments, the binding domain (e.g., scFv) and the extracellular domain of the PR are linked via a linker. 
     In some embodiments, the ECD antigen binding domain can bind to an intracellular antigen. In some embodiments, the intracellular antigen is a cancer antigen. 
     In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity of less than 1000 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity of less than 500 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity of less than 450 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity of less than 400 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity of less than 350 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity of less than 250 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity of less than 200 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity of less than 100 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity ranging between than 200 nM to 1000 nM. In some embodiments, the extracellular antigen binding domain binds to the target ligand with an affinity ranging between than 300 nM to 1.5 mM. In some embodiments, the antigen binding domain binds to the target ligand with an affinity &gt;200 nM, &gt;300 nM or &gt;500 nM. 
     Peptide Linker 
     In some embodiments, the extracellular antigen binding domains, scFvs are linked to the TM domain or other extracellular domains by a linker. In some embodiments, where there are more than one scfv at the extracellular antigen binding domain the more than scfvs are linked with each other by linkers. 
     In some embodiments, the linkers are flexible. In some embodiments, the linkers comprise a hinge region. Linkers are usually short peptide sequences. In some embodiments, the linkers are stretches of Glycine and one or more Serine residues. Other amino acids preferred for short peptide linkers include but are not limited to threonine (Thr), serine (Ser), proline (Pro), glycine (Gly), aspartic acid (Asp), lysine (Lys), glutamine (Gln), asparagine (Asn), and alanine (Ala) arginine (Arg), phenylalanine (Phe), glutamic acid (Glu). Of these Pro, Thr, and Gln are frequently used amino acids for natural linkers. Pro is a unique amino acid with a cyclic side chain which causes a very restricted conformation. Pro-rich sequences are used as interdomain linkers, including the linker between the lipoyl and E3 binding domain in pyruvate dehydrogenase (GA 2 PA 3 PAKQEA 3 PAPA 2 KAEAPA 3 PA 2 KA (SEQ ID NO: 75)). For the purpose of the disclosure, the empirical linkers may be flexible linkers, rigid linkers, and cleavable linkers. Sequences such as (G4S)x (where x is multiple copies of the moiety, designated as 1, 2, 3, 4, and so on) (SEQ ID NO: 76) comprise a flexible linker sequence. Other flexible sequences used herein include several repeats of glycine, e.g., (Gly)6 (SEQ ID NO: 77) or (Gly)8 (SEQ ID NO: 78). On the other hand, a rigid linker may be used, for example, a linker (EAAAK)x, where x is an integer, 1, 2, 3, 4 etc. (SEQ ID NO: 79) gives rise to a rigid linker. 
     In some embodiments, the linker comprises at least 2, or at least 3 amino acids. In some embodiments, the linker comprises 4 amino acids. In some embodiments, the linker comprises 5 amino acids. In some embodiments, the linker comprises 6 amino acids. In some embodiments, the linker comprises 7 amino acids. In some embodiments, the linker comprises 8 amino acids. In some embodiments, the linker comprises 9 amino acids. In some embodiments, the linker comprises 8 amino acids. In some embodiments, the linker comprises 10 amino acids. In some embodiments, the linker comprises 11 amino acids. In some embodiments, the linker comprises 12 amino acids. In some embodiments, the linker comprises 13 amino acids. In some embodiments, the linker comprises 14 amino acids. In some embodiments, the linker comprises 15 amino acids. In some embodiments, the linker comprises 16 amino acids. In some embodiments, the linker comprises 17 amino acids. In some embodiments, the linker comprises 18 amino acids. In some embodiments, the linker comprises 19 amino acids. In some embodiments, the linker comprises 20 amino acids. 
     As contemplated herein, any suitable ECD, TM or ICD domain can be cloned interchangeably in the suitable portion of any one of the CARP receptors described in the disclosure to obtain a protein with enhanced phagocytosis compared to an endogenous receptor. 
     Characteristics of the Fusion Proteins: 
     The CFP can structurally incorporate into the cell membrane of the cell in which it is expressed. Specific leader sequences in the nucleic acid construct, such as the signal peptide can be used to direct plasma membrane expression of the encoded protein. The transmembrane domain encoded by the construct can incorporate the expressed protein in the plasma membrane of the cell. 
     In some embodiments, the transmembrane domain comprises a TM domain of an FcRalpha receptor, which dimerizes with endogenous FcR-gamma receptors in the macrophages, ensuring macrophage specific expression. 
     The CFP can render the cell that expresses it as potently phagocytic. When the recombinant nucleic acid encoding the CFP is expressed in a cell, the cell can exhibit an increased phagocytosis of a target cell having the antigen of a target cell, compared to a cell not expressing the recombinant nucleic acid. When the recombinant nucleic acid is expressed in a cell, the cell can exhibit an increased phagocytosis of a target cell having the antigen of a target cell, compared to a cell not expressing the recombinant nucleic acid. In some embodiments, the recombinant nucleic acid when expressed in a cell, the cell exhibits at least 2-fold increased phagocytosis of a target cell having the antigen of a target cell, compared to a cell not expressing the recombinant nucleic acid. In some embodiments, the recombinant nucleic acid when expressed in a cell, the cell exhibits at least 3-fold, 4-fold, 5-fold, 6-fold, 7-fold, 8-fold, 9-fold, 10-fold, 20-fold 30-fold or at least 5-fold increased phagocytosis of a target cell having the antigen of a target cell, compared to a cell not expressing the recombinant nucleic acid. 
     In some embodiments, expression of SIRP-ΔICD enhances phagocytosis of the cell expressing it by 1.1 fold or more, 1.2 fold or more, 1.3 fold or more, q.4 fold or more, 1.5 fold or more, by 1.6 fold or more, 1.7 fold or more, 1.8 fold or more, 1.9 fold or more, 2 fold or more, 3 fold or more, 4 fold or more, 5 fold or more, 8 fold or more, 10 fold or more, 15 fold or more, 20 fold or more, 30 fold or more, 40 fold or more, 50 fold or more, 60 fold or more, 70 fold or more 80 fold or more, 90 fold or more, 100 fold or more, compared to a cell not expressing SIRP-ΔICD. 
     In some embodiments, the cells co-expressing SIRP-ΔICD and a CFP encoding a phagocytic receptor as described herein exhibits an augmented phagocytosis compared to a cell that does not express either of the proteins. In some embodiments, co-expressing SIRP-ΔICD and a CFP encoding a phagocytic receptor as described herein exhibits more than 2-fold, more than 3-fold, more than 4-fold, more than 5-fold, more than 6-fold, more than 7-fold, more than 8-fold, more than 9-fold, more than 10-fold, more than 20-fold, more than 30-fold, more than 40-fold, more than 50-fold, more than 60-fold, more than 70-fold, more than 80-fold, more than 90-fold, more than 100-fold, or more than 150-fold or more than 200-fold increase in phagocytic potential (measured in fold change of phagocytic index) compared to a cell that does not express either the SIRP-ΔICD or the CFP encoding a phagocytic receptor. 
     In some embodiments, expression of the any one of a CFP expressing a CD47 blocking extracellular domain of SIRPα and an intracellular domain of a phagocytic receptor augments phagocytic activity of a cell expressing it by at least 1.5 fold or more, 1.6 fold or more, 1.7 fold or more, 1.8 fold or more, 1.9 fold or more, 2 fold or more, 3 fold or more, 4 fold or more, 5 fold or more, 8 fold or more, 10 fold or more, 15 fold or more, 20 fold or more, 30 fold or more, 40 fold or more, 50 fold or more, 60 fold or more, 70 fold or more 80 fold or more, 90 fold or more, 100 fold or more, compared to a cell not expressing the CFP, or compared to a cell expressing SIRP-ΔICD. 
     In some embodiments, the enhancement in phagocytosis of target cells by a cell expressing either SIRP-ΔICD is highly increased compared to a phagocytic cell not expressing SIRP-ΔICD. 
     In some embodiments, the enhancement in phagocytosis of target cells by a cell expressing a CFP comprising a CD47 blocking extracellular domain of SIRPα and an intracellular domain of a phagocytic receptor is highly increased compared to a control phagocytic cell not expressing the fusion protein or a control phagocytic cell expressing the SIRP-ΔICD. 
     In some embodiments, when the recombinant nucleic acid described herein is expressed in a cell, the cell exhibits an increased cytokine production. The cytokine can comprise any one of: IL-1, IL-6, IL-12, IL-23, TNF, CXCL9, CXCL10, CXCL11, IL-18, IL-23, IL-27 and interferons. 
     In some embodiments, when the recombinant nucleic acid described herein is expressed in a cell, the cell exhibits an increased cell migration. 
     In some embodiments, when the recombinant nucleic acid described herein is expressed in a cell, the cell exhibits an increased immune activity. In some embodiments, when the recombinant nucleic acid is expressed in a cell, the cell exhibits an increased expression of MHC II. In some embodiments, when the recombinant nucleic acid is expressed in a cell, the cell exhibits an increased expression of CD80. In some embodiments, when the recombinant nucleic acid is expressed in a cell, the cell exhibits an increased expression of CD86. In some embodiments, when the recombinant nucleic acid is expressed in a cell, the cell exhibits an increased iNOS production. 
     In some embodiments, when the recombinant nucleic acid is expressed in a cell, the cell exhibits decreased trogocytosis of a target cell expressing the antigen of a target cell compared to a cell not expressing the recombinant nucleic acid. 
     In embodiments, the chimeric receptors may be glycosylated, pegylated, and/or otherwise post-translationally modified. In further embodiments, glycosylation, pegylation, and/or other posttranslational modifications may occur in vivo or in vitro and/or may be performed using chemical techniques. In additional embodiments, any glycosylation, pegylation and/or other posttranslational modifications may be N-linked or O-linked. In embodiments any one of the chimeric receptors may be enzymatically or functionally active such that, when the extracellular domain is bound by a ligand, a signal is transduced to polarize a macrophage. 
     In some embodiments, the chimeric fusion protein (CFP) comprises an extracellular domain (ECD) targeted to bind to CD5 (CD5 binding domain), for example, comprising a heavy chain variable region (VH) having an amino acid sequence as set forth in SEQ ID NO: 1. In some embodiments, the chimeric CFP comprises a CD5 binding heavy chain variable domain comprising an amino acid sequence that has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 1. In some embodiments, the extracellular domain (ECD) targeted to bind to CD5 (CD5 binding domain) comprises a light chain variable domain (V L ) having an amino acid sequence as set forth in SEQ ID NO: 2. In some embodiments, the chimeric CFP comprises a CD5 binding light chain variable domain comprising an amino acid sequence that has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 2. 
     In some embodiments, the CFP comprises an extracellular domain targeted to bind to HER2 (HER2 binding domain) having for example a heavy chain variable domain amino acid sequence as set forth in SEQ ID NO: 8 and a light chain variable domain amino acid sequence as set forth in SEQ ID NO: 9. In some embodiments, the CFP comprises a HER2 binding heavy chain variable domain comprising an amino acid sequence that has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 8. In some embodiments, the CFP comprises a HER2 binding light chain variable domain comprising an amino acid sequence that has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 9. 
     In some embodiments, the CFP comprises a hinge connecting the ECD to the transmembrane (TM). In some embodiments the hinge comprises the amino acid sequence of the hinge region of a CD8 receptor. In some embodiments, the CFP may comprise a hinge having the amino acid sequence set forth in SEQ ID NO: 7 (CD8a chain hinge domain). In some embodiments, the PFP hinge region comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 7. 
     In some embodiments, the CFP comprises a CD8 transmembrane region, for example having an amino acid sequence set forth in SEQ ID NO: 6. In some embodiments, the CFP TM region comprises an amino acid sequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 6. 
     In some embodiments, the CFP comprises an intracellular domain having an FcR domain. In some embodiments, the CFP comprises an FcR domain intracellular domain comprises an amino acid sequence set forth in SEQ ID NO: 3, or at least a sequence having 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 3. 
     In some embodiments, the CFP comprises an intracellular domain having a PI3K recruitment domain. In some embodiments the PI3K recruitment domain comprises an amino sequence set forth in SEQ ID NO: 4. In some embodiments the PI3K recruitment domain comprises an amino acid sequence that has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 4. 
     In some embodiments, the CFP comprises an intracellular domain having a CD40 intracellular domain. In some embodiments the CD40 ICD comprises an amino sequence set forth in SEQ ID NO: 5. In some embodiments the CD40 ICD comprises an amino acid sequence that has at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO: 5. 
     In some embodiments, the CD5 binding domain comprises an scFv comprising: (i) a variable heavy chain (V H ) sequence of SEQ ID NO: 1 or with at least 90% sequence identity to SEQ ID NO: 1; and (ii) a variable light chain (V L ) sequence of SEQ ID NO: 2 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 2. In some embodiments, the CD5 binding domain comprises an scFv comprising SEQ ID NO: 33 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 33. In some embodiments, the HER2 binding domain comprises an scFv comprising: (i) a variable heavy chain (V H ) sequence of SEQ ID NO: 8 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 8; and (ii) a variable light chain (V L ) sequence of SEQ ID NO: 9 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 9. In some embodiments, the CD5 binding domain comprises an scFv comprising SEQ ID NO: 32 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 32. In some embodiments, the CFP further comprises an intracellular domain, wherein the intracellular domain comprises one or more intracellular signaling domains, and wherein a wild-type protein comprising the intracellular domain does not comprise the extracellular domain. 
     In some embodiments, the extracellular domain further comprises a hinge domain derived from CD8, wherein the hinge domain is operatively linked to the transmembrane domain and the anti-CD5 binding domain. In some embodiments, the extracellular hinge domain comprises a sequence of SEQ ID NO: 7 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 7. 
     In some embodiments, the CFP comprises an extracellular domain fused to a transmembrane domain of SEQ ID NO: 30 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 30. In some embodiments, the CFP comprises an extracellular domain fused to a transmembrane domain of SEQ ID NO: 31 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 31. 
     In some embodiments, the transmembrane domain comprises a CD8 transmembrane domain. In some embodiments, the transmembrane domain comprises a sequence of SEQ ID NO: 6 or 29 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 6 or 29. In some embodiments, the transmembrane domain comprises a sequence of SEQ ID NO: 18 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 18. In some embodiments, the transmembrane domain comprises a sequence of SEQ ID NO: 34 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 34. In some embodiments, the transmembrane domain comprises a sequence of SEQ ID NO: 19 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 19. 
     In some embodiments, the CFP comprises one or more intracellular signaling domains that comprise a phagocytic signaling domain. In some embodiments, the phagocytosis signaling domain comprises an intracellular signaling domain derived from a receptor other than Megf10, MerTk, FcRα, and Bai1. In some embodiments, the phagocytosis signaling domain comprises an intracellular signaling domain derived from a receptor other than Megf10, MerTk, an FcR, and Bai1. In some embodiments, the phagocytosis signaling domain comprises an intracellular signaling domain derived from a receptor other than CD3ζ. In some embodiments, the phagocytosis signaling domain comprises an intracellular signaling domain derived from FcRγ, FcRα or FcRε. In some embodiments, the phagocytosis signaling domain comprises an intracellular signaling domain derived from CD3ζ. In some embodiments, the CFP comprises an intracellular signaling domain of any one of SEQ ID NOs: 3, 20, 27 and 28 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to any one of SEQ ID NOs: 3, 20, 27 and 28. In some embodiments, the one or more intracellular signaling domains further comprises a proinflammatory signaling domain. In some embodiments, the proinflammatory signaling domain comprises a PI3-kinase (PI3K) recruitment domain. In some embodiments, the proinflammatory signaling domain comprises a sequence of SEQ ID NO: 4 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 4. In some embodiments, the proinflammatory signaling domain is derived from an intracellular signaling domain of CD40. In some embodiments, the proinflammatory signaling domain comprises a sequence of SEQ ID NO: 5 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 5. In some embodiments, the CFP comprises an intracellular signaling domain of SEQ ID NO: 21 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 21. In some embodiments, the CFP comprises an intracellular signaling domain of SEQ ID NO: 23 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 23. 
     In some embodiments, the CFP comprises a sequence of SEQ ID NO: 14 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 14. In some embodiments, the CFP comprises a sequence of SEQ ID NO: 15 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 15. In some embodiments, the CFP comprises a sequence of SEQ ID NO: 16 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 16. In some embodiments, the CFP comprises a sequence of SEQ ID NO: 24 or with at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 24. In some embodiments, the CFP comprises a sequence of SEQ ID NO:25 or with at least 70%, 75%, 80%, 85%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO: 25. 
     In some embodiments, the CFP comprises: (a) an extracellular domain comprising: (i) a scFv that specifically binds CD5, and (ii) a hinge domain derived from CD8; a hinge domain derived from CD28 or at least a portion of an extracellular domain from CD68; (b) a CD8 transmembrane domain, a CD28 transmembrane domain, a CD2 transmembrane domain or a CD68 transmembrane domain; and (c) an intracellular domain comprising at least two intracellular signaling domains, wherein the at least two intracellular signaling domains comprise: (i) a first intracellular signaling domain derived from FcRα, FcRγ or FcRε, and (ii) a second intracellular signaling domain: (A) comprising a PI3K recruitment domain, or (B) derived from CD40. In some embodiments, the CFP comprises as an alternative (c) to the above: an intracellular domain comprising at least two intracellular signaling domains, wherein the at least two intracellular signaling domains comprise: (i) a first intracellular signaling domain derived from a phagocytic receptor intracellular domain, and (ii) a second intracellular signaling domain derived from a scavenger receptor phagocytic receptor intracellular domain comprising: (A) comprising a PI3K recruitment domain, or (B) derived from CD40. Exemplary scavenger receptors from which an intracellular signaling domain may be derived may be found in Table 2. In some embodiments, the CFP comprises and intracellular signaling domain derived from an intracellular signaling domain of an innate immune receptor. 
     In some embodiments, the recombinant polynucleic acid is an mRNA. In some embodiments, the recombinant polynucleic acid is a circRNA. In some embodiments, the recombinant polynucleic acid is a viral vector. In some embodiments, the recombinant polynucleic acid is delivered via a viral vector. 
     In some embodiments, the myeloid cell is a CD14+ cell, a CD14+/CD16− cell, a CD14+/CD16+ cell, a CD14−/CD16+ cell, CD14−/CD16− cell, a dendritic cell, an M0 macrophage, an M2 macrophage, an M1 macrophage or a mosaic myeloid cell/macrophage/dendritic cell. 
     In one aspect, provided herein is a method of treating cancer in a human subject in need thereof comprising administering a pharmaceutical composition to the human subject, the pharmaceutical composition comprising: (a) a myeloid cell comprising a recombinant polynucleic acid sequence, wherein the polynucleic acid sequence comprises a sequence encoding a chimeric fusion protein (CFP), the CFP comprising: (i) an extracellular domain comprising an anti-CD5 binding domain, and (ii) a transmembrane domain operatively linked to the extracellular domain; and (b) a pharmaceutically acceptable carrier; wherein the myeloid cell expresses the CFP. 
     In some embodiments, upon binding of the CFP to CD5 expressed by a target cancer cell of the subject killing or phagocytosis activity of the myeloid cell is increased by greater than 20% compared to a myeloid cell not expressing the CFP. In some embodiments, growth of a tumor is inhibited in the human subject. 
     In some embodiments, the cancer is a CD5+ cancer. In some embodiments, the cancer is leukemia, T cell lymphoma, or B cell lymphoma. In some embodiments, the CFP comprises one or more sequences shown in Table A and/or Table B below. 
     
       
         
           
               
             
               
                 TABLE A 
               
             
            
               
                   
               
               
                 Exemplary sequences of CFPs and domains thereof 
               
            
           
           
               
               
               
            
               
                 SEQ 
                   
                 Sequence 
               
               
                 ID NO 
                 PFP/Domain 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 Anti-CD5 heavy chain 
                 EIQLVQSGGGLVKPGGSVRISCAASGYTFTNYGMNWV 
               
               
                   
                 variable domain 
                 RQAPGKGLEWMGWINTHTGEPTYADSFKGRFTFSLDD 
               
               
                   
                   
                 SKNTAYLQINSLRAEDTAVYFCTRRGYDWYFDVWGQ 
               
               
                   
                   
                 GTTVTV 
               
               
                   
               
               
                 2 
                 Anti-CD5 light chain 
                 DIQMTQSPSSLSASVGDRVTITCRASQDINSYLSWFQQK 
               
               
                   
                 variable domain 
                 PGKAPKTLIYRANRLESGVPSRFSGSGSGTDYTLTISSLQ 
               
               
                   
                   
                 YEDFGIYYCQQYDESPWTFGGGTKLEIK 
               
               
                   
               
               
                 33 
                 Anti-CD5 scFv 
                 EIQLVQSGGGLVKPGGSVRISCAASGYTFTNYGMNWV 
               
               
                   
                   
                 RQAPGKGLEWMGWINTHTGEPTYADSFKGRFTFSLDD 
               
               
                   
                   
                 SKNTAYLQINSLRAEDTAVYFCTRRGYDWYFDVWGQ 
               
               
                   
                   
                 GTTVTV SSGGGGSGGGGSGGGGS DIQMTQSPSSLSASV 
               
               
                   
                   
                 GDRVTITCRASQDINSYLSWFQQKPGKAPKTLIYRANR 
               
               
                   
                   
                 LESGVPSRFSGSGSGTDYTLTISSLQYEDFGIYYCQQYD 
               
               
                   
                   
                 ESPWTFGGGTKLEIK 
               
               
                   
               
               
                 3 
                 FcRγ-chain intracellular 
                 LYCRRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYET 
               
               
                   
                 signaling domain 
                 LKHEKPPQ 
               
               
                   
               
               
                 20 
                 FcRγ-chain 
                 LYCRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYETL 
               
               
                   
                 intracellular 
                   
               
               
                   
                 signaling domain 
                 KHEKPPQ 
               
               
                   
               
               
                 27 
                 FcRγ-chain 
                 RLKIQVRKAAITSYEKSDGVYTGLSTRNQETYETLKHE 
               
               
                   
                 intracellular 
                   
               
               
                   
                 signaling domain 
                 KPPQ 
               
               
                   
               
               
                 28 
                 FcRγ-chain 
                 RLKIQVRKAAITSYEKSDGVYTGLSTRNQETYETLKHE 
               
               
                   
                 intracellular 
                   
               
               
                   
                 signaling domain 
                 KPPQ 
               
               
                   
               
               
                 4 
                 PI3K recruitment domain 
                 YEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENM 
               
               
                   
               
               
                 5 
                 CD40 intracellular 
                 KKVAKKPTNKAPHPKQEPQEINFPDDLPGSNTAAPVQE 
               
               
                   
                 domain 
                 TLHGCQPVTQEDGKESRISVQERQ 
               
               
                   
               
               
                 6 
                 CD8α chain 
                 IYIWAPLAGTCGVLLLSLVIT 
               
               
                   
                 transmembrane domain 
                   
               
               
                   
               
               
                 29 
                 CD8α chain 
                 IYIWAPLAGTCGVLLLSLVITLYC 
               
               
                   
                 transmembrane domain 
                   
               
               
                   
               
               
                 7 
                 CD8α chain hinge 
                 ALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQP 
               
               
                   
                 domain 
                 LSLRPEACRPAAGGAVHTRGLD 
               
               
                   
               
               
                 8 
                 Anti-HER2 heavy chain 
                 DIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQ 
               
               
                   
                 variable domain 
                 KPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSL 
               
               
                   
                   
                 QPEDFATYYCQQHYTTPPTFGQGTKVEIKRTGSTSGSG 
               
               
                   
                   
                 KPGSGEGSEVQLVE 
               
               
                   
               
               
                 9 
                 Anti-HER2 light chain 
                 LVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEW 
               
               
                   
                 variable domain 
                 VARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNS 
               
               
                   
                   
                 LRAEDTAVYYCSRWGGDGFYAMDVWGQGTLVTV 
               
               
                   
               
               
                 32 
                 Anti-HER2 scFv 
                 DIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQ 
               
               
                   
                   
                 KPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSL 
               
               
                   
                   
                 QPEDFATYYCQQHYTTPPTFGQGTKVEIKRTGSTSGSG 
               
               
                   
                   
                 KPGSGEGSEVQLVESSGGGGSGGGGSGGGGSLVQPGGS 
               
               
                   
                   
                 LRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTN 
               
               
                   
                   
                 GYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTA 
               
               
                   
                   
                 VYYCSRWGGDGFYAMDVWGQGTLVTV 
               
               
                   
               
               
                 17 
                 GMCSF Signal peptide 
                 MWLQSLLLLGTVACSIS 
               
               
                   
               
               
                 18 
                 CD28 transmembrane 
                 FWVLVVVGGVLACYSLLVTVAFIIFWV 
               
               
                   
                 domain 
                   
               
               
                   
               
               
                 34 
                 CD2 Transmembrane 
                 IYLIIGICGGGSLLMVFVALLVFYIT 
               
               
                   
                 domain 
                   
               
               
                   
               
               
                 19 
                 CD 68 transmembrane 
                 ILLPLIIGLILLGLLALVLIAFCII 
               
               
                   
                 domain 
                   
               
               
                   
               
               
                 21 
                 TNFR1 intracellular 
                 QRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSP 
               
               
                   
                 domain 
                 TPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVA 
               
               
                   
                   
                 PPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTD 
               
               
                   
                   
                 DPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQN 
               
               
                   
                   
                 GRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMD 
               
               
                   
                   
                 LLGCLEDIEEALCGPAALPPAPSLLR 
               
               
                   
               
               
                 22 
                 TNFR2 intracellular 
                 PLCLQREAKVPHLPADKARGTQGPEQQHLLITAPSSSSS 
               
               
                   
                 domain 
                 SLESSASALDRRAPTRNQPQAPGVEASGAGEARASTGS 
               
               
                   
                   
                 SDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTM 
               
               
                   
                   
                 GDTDSSPSESPKDEQVPFSKEECAFRSQLETPETLLGSTE 
               
               
                   
                   
                 EKPLPLGVPDAGMKPS 
               
               
                   
               
               
                 23 
                 MDA5 intracellular 
                 MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFL 
               
               
                   
                 domain 
                 PAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGW 
               
               
                   
                   
                 TREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDE 
               
               
                   
                   
                 YLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNR 
               
               
                   
                   
                 IAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTG 
               
               
                   
                   
                 NNELVQELTGSDCSESNAEIEN 
               
               
                   
               
               
                 30 
                 CD8α chain hinge 
                 ALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQP 
               
               
                   
                 domain + transmembrane 
                 LSLRPEACRPAAGGAVHTRGLDIYIWAPLAGTCGVLLL 
               
               
                   
                 domain 
                 SLVITLYC 
               
               
                   
               
               
                 31 
                 CD8α chain hinge 
                 ALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQP 
               
               
                   
                 domain + transmembrane 
                 LSLRPEACRPAAGGAVHTRGLDIYIWAPLAGTCGVLLL 
               
               
                   
                 domain 
                 SLVIT 
               
               
                   
               
               
                 14 
                 CD5-FcRγ-PI3K 
                 MWLQSLLLLGTVACSISEIQLVQSGGGLVKPGGSVRISC 
               
               
                   
                   
                 AASGYTFTNYGMNWVRQAPGKGLEWMGWINTHTGEP 
               
               
                   
                   
                 TYADSFKGRFTFSLDDSKNTAYLQINSLRAEDTAVYFC 
               
               
                   
                   
                 TRRGYDWYFDVWGQGTTVTV SSGGGGSGGGGSGGGG   
               
               
                   
                   
                   S DIQMTQSPSSLSASVGDRVTITCRASQDINSYLSWFQQ 
               
               
                   
                   
                 KPGKAPKTLIYRANRLESGVPSRFSGSGSGTDYTLTISSL 
               
               
                   
                   
                 QYEDFGIYYCQQYDESPWTFGGGTKLEIKSGGGGSGAL 
               
               
                   
                   
                 SNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSL 
               
               
                   
                   
                 RPEACRPAAGGAVHTRGLDIYIWAPLAGTCGVLLLSLV 
               
               
                   
                   
                 ITLYCRRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYE 
               
               
                   
                   
                 TLKHEKPPQGSGSYEDMRGILYAAPQLRSIRGQPGPNHE 
               
               
                   
                   
                 EDADSYENM 
               
               
                   
               
               
                 15 
                 HER2-FcRγ-PD3K 
                 MWLQSLLLLGTVACSISDIQMTQSPSSLSASVGDRVTIT 
               
               
                   
                   
                 CRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVP 
               
               
                   
                   
                 SRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFG 
               
               
                   
                   
                 QGTKVEIKRTGSTSGSGKPGSGEGSEVQLVESGGGLVQ 
               
               
                   
                   
                 PGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARI 
               
               
                   
                   
                 YPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAE 
               
               
                   
                   
                 DTAVYYCSRWGGDGFYAMDVWGQGTLVTVSSSGGGG 
               
               
                   
                   
                 SGALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIAS 
               
               
                   
                   
                 QPLSLRPEACRPAAGGAVHTRGLDIYIWAPLAGTCGVL 
               
               
                   
                   
                 LLSLVITLYCRRLKIQVRKAAITSYEKSDGVYTGLSTRN 
               
               
                   
                   
                 QETYETLKHEKPPQGSGSYEDMRGILYAAPQLRSIRGQP 
               
               
                   
                   
                 GPNHEEDADSYENM 
               
               
                   
               
               
                 16 
                 CD5-FcRγ-CD40 
                 MWLQSLLLLGTVACSISEIQLVQSGGGLVKPGGSVRISC 
               
               
                   
                   
                 AASGYTFTNYGMNWVRQAPGKGLEWMGWINTHTGEP 
               
               
                   
                   
                 TYADSFKGRFTFSLDDSKNTAYLQINSLRAEDTAVYFC 
               
               
                   
                   
                 TRRGYDWYFDVWGQGTTVTVSSGGGGSGGGGSGGGG 
               
               
                   
                   
                 SDIQMTQSPSSLSASVGDRVTITCRASQDINSYLSWFQQ 
               
               
                   
                   
                 KPGKAPKTLIYRANRLESGVPSRFSGSGSGTDYTLTISSL 
               
               
                   
                   
                 QYEDFGIYYCQQYDESPWTFGGGTKLEIKSGGGGSGAL 
               
               
                   
                   
                 SNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSL 
               
               
                   
                   
                 RPEACRPAAGGAVHTRGLDIYIWAPLAGTCGVLLLSLV 
               
               
                   
                   
                 ITLYCRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYET 
               
               
                   
                   
                 LKHEKPPQKKVAKKPTNKAPHPKQEPQEINFPDDLPGS 
               
               
                   
                   
                 NTAAPVQETLHGCQPVTQEDGKESRISVQERQ 
               
               
                   
               
               
                 24 
                 CD5-FcRγ-MDA5 
                 MWLQSLLLLGTVACSISEIQLVQSGGGLVKPGGSVRISC 
               
               
                   
                   
                 AASGYTFTNYGMNWVRQAPGKGLEWMGWINTHTGEP 
               
               
                   
                   
                 TYADSFKGRFTFSLDDSKNTAYLQINSLRAEDTAVYFC 
               
               
                   
                   
                 TRRGYDWYFDVWGQGTTVTVSSGGGGSGGGGSGGGG 
               
               
                   
                   
                 SDIQMTQSPSSLSASVGDRVTITCRASQDINSYLSWFQQ 
               
               
                   
                   
                 KPGKAPKTLIYRANRLESGVPSRFSGSGSGTDYTLTISSL 
               
               
                   
                   
                 QYEDFGIYYCQQYDESPWTFGGGTKLEIKSGGGGSGAL 
               
               
                   
                   
                 SNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSL 
               
               
                   
                   
                 RPEACRPAAGGAVHTRGLDIYIWAPLAGTCGVLLLSLV 
               
               
                   
                   
                 ITLYCRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYET 
               
               
                   
                   
                 LKHEKPPQGSGSMSNGYSTDENFRYLISCFRARVKMYI 
               
               
                   
                   
                 QVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLS 
               
               
                   
                   
                 TLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTD 
               
               
                   
                   
                 LPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCM 
               
               
                   
                   
                 EEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENW 
               
               
                   
                   
                 FSAFLNVLRQTGNNELVQELTGSDCSESNAEIEN 
               
               
                   
               
               
                 25 
                 CD5-FcRγ-TNFR1 
                 MWLQSLLLLGTVACSISEIQLVQSGGGLVKPGGSVRISC 
               
               
                   
                   
                 AASGYTFTNYGMNWVRQAPGKGLEWMGWINTHTGEP 
               
               
                   
                   
                 TYADSFKGRFTFSLDDSKNTAYLQINSLRAEDTAVYFC 
               
               
                   
                   
                 TRRGYDWYFDVWGQGTTVTVSSGGGGSGGGGSGGGG 
               
               
                   
                   
                 SDIQMTQSPSSLSASVGDRVTITCRASQDINSYLSWFQQ 
               
               
                   
                   
                 KPGKAPKTLIYRANRLESGVPSRFSGSGSGTDYTLTISSL 
               
               
                   
                   
                 QYEDFGIYYCQQYDESPWTFGGGTKLEIKSGGGGSGAL 
               
               
                   
                   
                 SNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSL 
               
               
                   
                   
                 RPEACRPAAGGAVHTRGLDIYIWAPLAGTCGVLLLSLV 
               
               
                   
                   
                 ITLYCRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYET 
               
               
                   
                   
                 LKHEKPPQGSGSQRWKSKLYSIVCGKSTPEKEGELEGT 
               
               
                   
                   
                 TTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGD 
               
               
                   
                   
                 CPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWE 
               
               
                   
                   
                 DSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLG 
               
               
                   
                   
                 LSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREAT 
               
               
                   
                   
                 LELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR 
               
               
                   
               
               
                 26 
                 CD5-FcRγ-TNFR2 
                 MWLQSLLLLGTVACSISEIQLVQSGGGLVKPGGSVRISC 
               
               
                   
                   
                 AASGYTFTNYGMNWVRQAPGKGLEWMGWINTHTGEP 
               
               
                   
                   
                 TYADSFKGRFTFSLDDSKNTAYLQINSLRAEDTAVYFC 
               
               
                   
                   
                 TRRGYDWYFDVWGQGTTVTVSSGGGGSGGGGSGGGG 
               
               
                   
                   
                 SDIQMTQSPSSLSASVGDRVTITCRASQDINSYLSWFQQ 
               
               
                   
                   
                 KPGKAPKTLIYRANRLESGVPSRFSGSGSGTDYTLTISSL 
               
               
                   
                   
                 QYEDFGIYYCQQYDESPWTFGGGTKLEIKSGGGGSGAL 
               
               
                   
                   
                 SNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSL 
               
               
                   
                   
                 RPEACRPAAGGAVHTRGLDIYIWAPLAGTCGVLLLSLV 
               
               
                   
                   
                 ITLYCRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYET 
               
               
                   
                   
                 LKHEKPPQGSGSPLCLQREAKVPHLPADKARGTQGPEQ 
               
               
                   
                   
                 QHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEAS 
               
               
                   
                   
                 GAGEARASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHS 
               
               
                   
                   
                 SQCSSQASSTMGDTDSSPSESPKDEQVPFSKEECAFRSQ 
               
               
                   
                   
                 LETPETLLGSTEEKPLPLGVPDAGMKPS 
               
               
                   
               
            
           
         
       
     
                     TABLE B                  Linker sequences                             SEQ ID   Sequence                       10   SSGGGGSGGGGSGGGGS                       11   SGGGGSG                       12   SGGG                       13   GSGS                        
IV. Noncoding Exogenous Sequence for Delivery and Incorporation into the Genome of a Cell.
 
     A noncoding sequence may be delivered into the cell and designed to be incorporated in the genome of the cell. The noncoding sequence as used herein, is a sequence that does not result in a translated protein product, but may have regulatory elements, such as transcribed products, such as inhibitory RNA. 
     In some embodiments, such a sequence may be a miRNA sequence. In some embodiments, the sequence may be a sequence for siRNA generation. In some embodiments, the sequence may comprise an intronic sequence, or a binding site created such that one or more DNA binding proteins can dock on the site and influence the nature and behavior of the adjoining regions. In some embodiments, the sequence may be a transcription factor binding site. In some embodiments, the sequence may comprise an enhancer binding site. In some embodiments, the sequence may comprise a binding site for topoisomerase, gyrase, reverse transcriptase, polymerase, poly A binding protein, guanylyl cyclase, ligase, restriction enzymes, DNA methylase, HDAC enzymes, and many others. In some embodiments, the noncoding sequence may be directed to manipulating heterochromatin. A noncoding insert sequence, as it may also be referred to here, may be a few nucleotides to 5 kB in length. 
     V. Plasmid Design and Recombinant Nucleic Acid Design Comprising an Insert Sequence 
     The nucleic acid construct comprising one or more sequences encoding one or more proteins or polypeptides is incorporated in a plasmid for transcription and generating an mRNA. mRNA can be transcribed in an in vitro system using synthetic system of cell extracts. Alternatively, mRNA can be generated in a cell and harvested. The cell can be a prokaryotic cell, such as a bacterial cell. In some embodiments, the cell is a eukaryotic cell. In some embodiments, the transcription occurs in a synthetic system. Provided herein are exemplary plasmid design. 
     In some embodiments, of the various aspects of the disclosure, a plasmid is designed for expression of the mRNA molecule comprising a heterologous sequence of interest that encodes a protein or a polypeptide. The plasmid comprises, inter alia, the sequences for genomic integration elements for integration of the heterologous sequence of interest that encodes a protein or a polypeptide; the sequence comprising the transgene or fragment thereof, operably linked to its separate promoter and regulatory elements that are required for its expression in the host following integration in the host genome, (such as, the subject who is administered the mRNA); one or more regulatory elements for transcription and generation of the mRNA including a promoter for expression of the mRNA, e.g. in a bacterial cell or cell extract, and 3′ stabilizing elements; sequences for one or more detection marker and/or selection markers. 
     As is known to one of skill in the art, a plasmid backbone can be an available vector, such as an in-house or commercially developed vector, that can be improved in various ways for best expression of the transcribed sequences, for example, (but not limited to), by introducing one or more desirable restriction digestion sites in the MCS (multiple cloning site), introducing a desired promoter for overall mRNA transcription, such as the T7 promoter, exchanging an existing sequence within the plasmid vector for one or more desired sequences, or introducing one or more desired segments, such as a selection marker sequence. 
     The plasmid comprises transcription regulatory elements, such as a promoter at the 5′ region, and a 3′-stabilizing element. In some embodiments, the promoter is chosen for enhanced mRNA transcription in the desired cell, such as an  E. coli  bacterial cell. In some embodiments, the promoter for transcription of the plasmid is selected from a T7 promoter, a Sp6 promoter, pL (lambda) promoter, T3 promoter, trp promoter, araBad promoter, lac promoter or a Ptac promoter. In some embodiments, the promoter is a T7 promoter. T7 or Sp6 promoters are constitutive promoters and are useful for high level transcription or in vitro transcription. In some embodiments, the 3′ stabilizing element is a sequence from BGH 3′ element, WPRE 3′ element, SV40 element, hGH element and other elements. The 3′ element comprises the necessary poly A and transcription termination sequences. 
     Exemplary selection markers include antibiotic selection marker and/or expression detection marker. Antibiotic selection markers include but are not limited to ampicillin resistance gene sequence (beta lactamase gene or fragment thereof) conferring resistance to ampicillin, for example G418 selection marker, tetracycline resistance gene sequence conferring resistance to tetracycline, kanamycin resistance gene sequence conferring resistance to kanamycin, erythromycin resistance gene sequence conferring resistance to erythromycin, chloramphenicol resistance gene sequence conferring resistance to chloramphenicol, neomycin resistant gene sequence conferring resistance to neomycin, and others. Exemplary expression detection marker include FLAG, HA, GFP and others. 
     In some embodiments, the and other tags that can be fused to one or more coding sequences to function as a surrogate for the expression of the desired protein or peptide to which it is fused. 
     In some embodiments, the plasmid is less than 20 kb in length. In some embodiments, the plasmid is less than 19 kb in length. In some embodiments, the plasmid is less than 20 kb in length. In some embodiments, the plasmid is less than 18 kb in length. In some embodiments, the plasmid is less than 20 kb in length. In some embodiments, the plasmid is less than 17 kb in length. In some embodiments, the plasmid is less than 20 kb in length. In some embodiments, the plasmid is less than 16 kb in length. In some embodiments, the plasmid is less than 15 kb in length. In some embodiments, the plasmid is less than 14 kb in length. In some embodiments, the plasmid is less than 13 kb in length. In some embodiments, the plasmid is less than 12 kb in length. In some embodiments, the plasmid is about 15 kb, about 14 kb, about 13 kb, about 12 kb or about 10 kb in length. 
     In some embodiments, the codon is optimized for maximized transcription suitable for the transcription system. 
     VI. Features Related to the Expression of the Transgene In Vivo 
     Transcription Regulatory Elements in the Recombinant Nucleic Acid Construct (Transgene) 
     In some embodiments, the recombinant nucleic comprises one or more regulatory elements within the noncoding regions that can be manipulated for desired expression profiles of the encoded proteins. In some embodiments, the noncoding region may comprise suitable enhancer. In some embodiments, the enhancer comprises a binding region for a regulator protein or peptide may be added to the cell or the system comprising the cell, for commencement of expression of the protein encoded under the influence of the enhancer. Conversely, a regulatory element may comprise a protein binding domain that remains bound with the cognate protein and continue to inhibit transcription and/or translation of recombinant protein until an extracellular signal is provided for the protein to decouple from the bound position to allow commencement of the protein synthesis. Examples include but are not limited to Tetracycline-inducible (Tet-Inducible or Tet-on) and Tetracycline repressible (Tet-off) systems known to one of skill in the art. 
     Construct comprising metabolic switch: In some embodiments, the 5′ and 3′ untranslated regions flanking the coding regions of the construct may be manipulated for regulation of expression of the recombinant protein encoded by the nucleic acid constructs described above. For instance, the 3′UTR may comprise one or more elements that are inserted for stabilizing the mRNA. In some embodiments, AU-Rich Elements (ARE) sequences are inserted in the 3′ UTR that result in binding of RNA binding proteins that stabilize or destabilize the mRNA, allowing control of the mRNA half-life. 
     In some embodiments, the 3′UTR may comprise a conserved region for RNA binding proteins (e.g. GAPDH) binding to mature mRNA strand preventing translation. In some embodiments, glycolysis results in the uncoupling of the RNA binding proteins (e.g. GAPDH) allowing for mRNA strand translation. The principle of the metabolic switch is to trigger expression of target genes when a cell enters a certain metabolic state. In resting cells, for example, GAPDH is an RNA binding protein (RBP). It binds to ARE sequences in the 3′UTR, preventing translation of mRNA. When the cell enters glycolysis, GAPDH is required to convert glucose into ATP, coming off the mRNA allowing for translation of the protein to occur. In some embodiments, the environment in which the cell comprising the recombinant nucleic acid is present, provides the metabolic switch to the gene expression. For example, hypoxic condition can trigger the metabolic switch inducing the disengaging of GAPDH from the mRNA. The expression of the mRNA therefore can be induced only when the macrophage leaves the circulation and enters into a tumor environment, which is hypoxic. This allows for systemic administration of the nucleic acid or a cell comprising the nucleic acid, but ensures a local expression, specifically targeting the tumor environment. 
     In some embodiments, the nucleic acid construct can be a split construct, for example, allowing a portion of the construct to be expressed under the control of a constitutive expression system whereas another portion of the nucleic acid is expressed under control of a metabolic switch, as described above. In some embodiments, the nucleic acid may be under bicistronic control. In some embodiments, the bicistronic vector comprises a first coding sequence under a first regulatory control, comprising the coding sequence of a target recognition moiety which may be under constitutive control; and a second coding sequence encoding an inflammatory gene expression which may be under the metabolic switch. In some embodiments, the bicistronic vector may be unidirectional. In some embodiments, the bicistronic vector may be bidirectional. 
     In some embodiments, the ARE sequences comprise protein binding motifs for binding ARE sequence that bind to ADK, ALDH18A1, ALDH6A1, ALDOA, ASS1, CCBL2, CS, DUT, ENO1, FASN, FDPS, GOT2, HADHB, HK2, HSD17B10, MDH2, NME1, NQ01, PKM2, PPP1CC, SUCLG1, TP11, GAPDH, or LDH. 
     Pharmaceutical Compositions and Immunotherapy 
     In one aspect provided herein is a pharmaceutical composition comprising (i) the nucleic acid encoding the transgene is incorporated in a transpositioning or retrotranspositioning system comprising the transgene, the 5′- and 3′-flanking transposition or retrotranspositioning elements, the expression regulation elements, such as promoters, introns; and a nucleic acid encoding the transposase or retrotransposase, (ii) a nucleic acid delivery vehicle and a pharmaceutically acceptable salt or excipient. 
     In some embodiments, the pharmaceutical composition comprises cells comprising the nucleic acid encoding the transgene that is stably integrated in the genome of the cell and a pharmaceutically acceptable excipient. Nucleic acid constructs can be delivered with cationic lipids (Goddard, et al, Gene Therapy, 4:1231-1236, 1997; Gorman, et al, Gene Therapy 4:983-992, 1997; Chadwick, et al, Gene Therapy 4:937-942, 1997; Gokhale, et al, Gene Therapy 4:1289-1299, 1997; Gao, and Huang, Gene Therapy 2:710-722, 1995), using viral vectors (Monahan, et al, Gene Therapy 4:40-49, 1997; Onodera, et al, Blood 91:30-36, 1998), by uptake of “naked DNA”, and the like. Techniques well known in the art for the transformation of cells (see discussion above) can be used for the ex vivo administration of nucleic acid constructs. The exact formulation, route of administration and dosage can be chosen empirically. (See e.g. Fingl et al., 1975, in “The Pharmacological Basis of Therapeutics”, Ch. 1 pl). 
     In some embodiments, the nucleic acid comprising the transgene and the transposable elements is introduced or incorporated in the cell by known methods of nucleic acid transfer inside a cell, such as using lipofectamine, or calcium phosphate, or via physical means such as electroporation or nucleofection. 
     In some embodiments, the nucleic acid is encapsulated in liposomes or lipid nanoparticles. LNPs are 100-300 nm in diameter provide efficient means of mRNA delivery to various cell types, including macrophages. In some embodiments, the nucleic acid is transferred by other nanoparticles. In some embodiments, the vector for expression of the CFP is of a viral origin, namely a lentiviral vector or an adenoviral vector. In some embodiments, the nucleic acid encoding the recombinant nucleic acid is encoded by a lentiviral vector. In some embodiments, the lentiviral vector is prepared in-house and manufactured in large scale for the purpose. In some embodiments, commercially available lentiviral vectors are utilized, as is known to one of skill in the art. 
     In some embodiments, the viral vector is an Adeno-Associated Virus (AAV) vector. 
     The methods find use in a variety of applications in which it is desired to introduce an exogenous nucleic acid into a target cell and are particularly of interest where it is desired to express a protein encoded by an expression cassette in a target cell, where the target cell or cells are part of a multicellular organism. The transposase system may be administered to the organism or host in a manner such that the targeting construct is able to enter the target cell(s), e.g., via an in vivo or ex vivo protocol. Such cells or organs are typically returned to a living body. 
     In some embodiments, the transgene encoding a fusion protein related to immune function is stably integrated in a living cell of a subject ex vivo, following which the cell comprising the transgene is returned to the subject. Of exemplary importance, the CFP transgene (phagocytic receptor fusion protein) is intended for expression in an immune cell, such as a myeloid cell, a phagocytic cell, a macrophage, a monocyte or a cell of dendritic cell lineage is contacted ex vivo with the recombinant nucleic acids for stable transfer of the transgene and re-introduced in the same subject for combating a disease of the subject. The diseases contemplated comprises infectious diseases, cancer and autoimmune diseases. The nucleic acid encoding the PSR subunit comprising fusion protein (CFP) described herein is used to generate engineered phagocytic cells for treating cancer. 
     Cancers include, but are not limited to T cell lymphoma, cutaneous lymphoma, B cell cancer (e.g., multiple myeloma, Waldenstrom&#39;s macroglobulinemia), the heavy chain diseases (such as, for example, alpha chain disease, gamma chain disease, and mu chain disease), benign monoclonal gammopathy, and immunocytic amyloidosis, melanomas, breast cancer, lung cancer, bronchus cancer, colorectal cancer, prostate cancer (e.g., metastatic, hormone refractory prostate cancer), pancreatic cancer, stomach cancer, ovarian cancer, urinary bladder cancer, brain or central nervous system cancer, peripheral nervous system cancer, esophageal cancer, cervical cancer, uterine or endometrial cancer, cancer of the oral cavity or pharynx, liver cancer, kidney cancer, testicular cancer, biliary tract cancer, small bowel or appendix cancer, salivary gland cancer, thyroid gland cancer, adrenal gland cancer, osteosarcoma, chondrosarcoma, cancer of hematological tissues, and the like. Other non-limiting examples of types of cancers applicable to the methods encompassed by the present disclosure include human sarcomas and carcinomas, e.g., fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma, endotheliosarcoma, lymphangiosarcoma, lymphangioendotheliosarcoma, synovioma, mesothelioma, Ewing&#39;s tumor, leiomyosarcoma, rhabdomyosarcoma, colon carcinoma, colorectal cancer, pancreatic cancer, breast cancer, ovarian cancer, squamous cell carcinoma, basal cell carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinomas, cystadenocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, liver cancer, choriocarcinoma, seminoma, embryonal carcinoma, Wilms&#39; tumor, cervical cancer, bone cancer, brain tumor, testicular cancer, lung carcinoma, small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, hemangioblastoma, acoustic neuroma, oligodendroglioma, meningioma, melanoma, neuroblastoma, retinoblastoma; leukemias, e.g., acute lymphocytic leukemia and acute myelocytic leukemia (myeloblastic, promyelocytic, myelomonocytic, monocytic and erythroleukemia); chronic leukemia (chronic myelocytic (granulocytic) leukemia and chronic lymphocytic leukemia); and polycythemia vera, lymphoma (Hodgkin&#39;s disease and non-Hodgkin&#39;s disease), multiple myeloma, Waldenstrom&#39;s macroglobulinemia, and heavy chain disease. In some embodiments, the cancer is an epithelial cancer such as, but not limited to, bladder cancer, breast cancer, cervical cancer, colon cancer, gynecologic cancers, renal cancer, laryngeal cancer, lung cancer, oral cancer, head and neck cancer, ovarian cancer, pancreatic cancer, prostate cancer, or skin cancer. In other embodiments, the cancer is breast cancer, prostate cancer, lung cancer, or colon cancer. In still other embodiments, the epithelial cancer is non-small-cell lung cancer, nonpapillary renal cell carcinoma, cervical carcinoma, ovarian carcinoma (e.g., serous ovarian carcinoma), or breast carcinoma. The epithelial cancers can be characterized in various other ways including, but not limited to, serous, endometrioid, mucinous, clear cell, or undifferentiated. In some embodiments, the present disclosure is used in the treatment, diagnosis, and/or prognosis of lymphoma or its subtypes, including, but not limited to, mantle cell lymphoma. Lymphoproliferative disorders are also considered to be proliferative diseases. 
     In general, cellular immunotherapy comprises providing the patient a medicament comprising live cells, which should be HLA matched for compatibility with the subject, and such that the cells do not lead to graft versus Host Disease, GVHD. A subject arriving at the clinic for personalized medicine and immunotherapy as described above, is routinely HLA typed for determining the HLA antigens expressed by the subject. 
     Therapeutic Advantages of mRNA Driven Delivery 
     In one embodiment, provided herein is a method of introducing a nucleic acid sequence into a cell for sustained gene expression in the cell without adverse effects. In some embodiments, the cell is within a living system, e.g., a host organism such as a human. The nucleic acid sequence is an mRNA. 
     In particular, delivery via retrotransposon poses to be a highly lucrative mode. mRNA driven delivery simplifies gene delivery. While other technologies require expensive and sophisticated design and manufacturing, and a solution for delivery of the nucleic acid into the cell, and gene editing technologies to assist in integration, retrotransposon mediated delivery itself encodes for the editing machinery, encodes for new genes to be delivered. In addition, a single mRNA may be sufficient for gene delivery and editing. 
     In one embodiment, mRNA delivery is advantageous in that it can ensure introduction of a nucleic acid cargo without size restraint. 
     Table 9 summarizes some of the advantages over the other existing methods of nucleic acid deliveries. 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Advantages of retrotransposon mediated gene delivery 
               
            
           
           
               
               
               
               
            
               
                   
                 Lentiviral 
                   
                 Retrotransposon 
               
               
                   
                 delivery 
                 AAV-delivery 
                 delivery 
               
               
                   
                   
               
            
           
           
               
               
               
               
            
               
                 Payload 
                 ~4 kb 
                 ~4 kb 
                 &gt;10 kb 
               
               
                 Toxicity 
                 Insertional 
                 Unresolved 
                 Unknown, 
               
               
                   
                 mutagenesis 
                 liver &amp; CNS 
                 pending 
               
               
                   
                   
                 toxicity 
                 clinical 
               
               
                   
                   
                   
                 development 
               
               
                 Manufacturing 
                 Complex, 
                 Complex, 
                 Inexpensive, 
               
               
                   
                 expensive 
                 expensive 
                 rapid 
               
               
                   
               
            
           
         
       
     
     Retrotransposons are advantageous for applications across multiple modalities. Gene manipulation using this method is easily attained both in vivo and ex vivo. In one embodiment, the application of retrotransposon may be in vivo, a piece of genetic material encoded in an mRNA can be directly introduced into a patient by systemic or local introduction. In contrast, cells can be taken out from a subject, and manipulated ex vivo and then introduced either to the same subject (autologous) or to another human (heterologous). 
     In one embodiment, retrotransposons and the related methods described herein may be instrumental in gene therapy. With the advantage of capacity to introduce large payloads, large sections of DNA carrying a gene encoding an entire protein may be introduced in one shot without requiring multiple introductions and multiple editing events. In one embodiment, for example, a gene that encodes a defective protein may be excised, the correct gene may be introduced in the correct site in one integration event using a retrotransposon mediated delivery. In one example, CRISPR editing may be used to excise a gene from precise locus and retrotransposition may be used to replace the correct genes. In some embodiments, a preferred retrotransposon integration site may be introduced at the excision site. 
     In one embodiment, retrotransposons and the related methods described herein may be instrumental in gene editing. 
     In one embodiment, retrotransposons and the related methods described herein may be instrumental in transcriptional regulation. 
     In one embodiment, retrotransposons and the related methods described herein may be instrumental in genome engineering. 
     In one embodiment, provided herein is a composition for incorporating a heterologous nucleic acid sequence in the genome comprising one or more polynucleic acids further comprise (i) a sequence encoding an integrase or a fragment thereof for site directed integration of the insert sequence into the genome and (ii) a genome landing site sequence that operable by the integrase, wherein the genome landing sequence is greater than 4, 5, 6, 7, 8, 9, or 10 consecutive nucleotides long. In some embodiments the integrase is a bacterial integrase. In some embodiments, the integrase is a serine integrase. 
     In some embodiments, the composition comprises an ORF2 and an integrase or a fragment thereof, wherein the integrase and the ORF2 are on separate polynucleotides. In some embodiments, the integrase has a capability of integrating nucleic acid sequence into a genomic site that has a genomic landing sequence that is about 10 nucleotides long, about 20 nucleotides long, about 30 nucleotides long, about 40 nucleotides long. 
     In some embodiments, the ORF2 and the integrase are on a single polynucleotide. In some embodiments, the ORF2 is modified to incorporate a fragment of an integrase protein that can recognize a genomic landing sequence of greater than 10 consecutive nucleotides long, and wherein the LINE retrotransposon system integrates the heterologous genomic insert into the genomic landing sequence recognized by the fragment of an integrase protein that has been incorporated into the genome. 
     In some embodiments, the integrase is not integrated into the genome of the cell. In some embodiments, the integrase is a recombinant protein. In some embodiments the ORF protein, e.g., the ORF2 protein is a recombinant (e.g., chimeric) protein, which comprises at least a fragment of a bacterial serine integrase that is capable of recognizing a genomic landing sequence of an integrase, e.g., a bacterial integrase, e.g., a bacterial serine integrase, wherein the genome landing sequence that is operable by the integrase and is greater than 20 nucleotides long, or greater than 30 nucleotides long. In some embodiments, the one or more of the ORF protein sequence comprise a mutation. In some embodiments, the recombinant (e.g., chimeric) ORF protein comprises a deletion of the target recognition sequence. In some embodiments, the recombinant (e.g., chimeric) ORF protein comprises a fragment of the integrase, e.g., a bacterial integrase, e.g., a bacterial serine integrase, which comprises a catalytic domain or a fragment thereof, a site-specific genomic integration recognition domain. 
     Provided herein is a pharmaceutical composition, wherein the insert sequence comprises an attachment site operable by the integrase. 
     In some embodiments, the genomic landing site is inserted into the genome using a guide RNA and a Cas system. In some embodiments, the guide RNA, the CAS system and the genomic landing sequence are in a polynucleotide that is separate from the polynucleotide comprising the sequence encoding the LINE1-ORFs and the insert sequence. 
     Provided herein is a method for a site-specific integration of a heterologous genomic insert sequence into the genome of a mammalian cell, the method comprising: (i) introducing into the cell (a) a polynucleotide comprising sequences encoding one or more human retrotransposon elements associated with the heterologous insert sequence, and (b) a polynucleotide comprising sequence encoding a guide RNA, an RNA guided integrase or a fragment thereof and a landing sequence operable by the integrase; (ii) verifying the integration of the heterologous insert sequence into the site of the genome. 
     Provided herein is a method for site-specific integration of a heterologous genomic insert using a LINE retrotransposon system, wherein the LINE retrotransposon system is modified to incorporate a fragment of an integrase protein that can recognize a genomic landing sequence of greater than 10 consecutive nucleotides long, and wherein the LINE retrotransposon system integrates the heterologous genomic insert into the genomic landing sequence recognized by the fragment of the integrase protein that has been incorporated into the genome. In some embodiments, the integrase recognizes and contacts the genomic landing sequence, and the reverse transcriptase elements of the LINE (L1) retrotransposon system reverse transcribes and incorporates the insert sequence (e.g., the cargo sequence) at the specific site of the genome landing sequence. In some embodiments, the method comprises a step of incorporating into the genome the genomic landing sequence of greater than 10 consecutive nucleotides long. In some embodiments, the step of incorporating into the genome the genomic landing sequence is performed by an RNA-guided CRISPR-Cas system. In some embodiments, the RNA-guided CRISPR-Cas system has an editing function capable of incorporating a sequence of greater than 10 consecutive nucleotides long into a specific genome site. 
     Therefore, provided herein is a modified L1 retrotransposition system comprising a site specific integrase DNA recognition moiety; wherein the integrase DNA recognition moiety recognizes the genomic sequence for site-specific integration, and wherein the L1 retrotransposition system reverse transcribes and integrates the sequence comprising the heterologous insert into the genomic site at or near the site recognized by the integrase. Existing systems incorporating an integrase, such as a serine integrase may comprise a DNA integration system, using for example a plasmid, a viral delivery system, each of which can be bypassed for the safer and sure mRNA system as used herein, and without the limitation of cargo size. 
     In one embodiment, retrotransposons and the related methods described herein may be instrumental in developing cell therapy, for example chimeric antigen receptor (CAR)T cells, in NK cell therapy or in myeloid cell therapy. In one embodiment, retrotransposons and the related methods described herein may be instrumental in delivery of genes into neurons, which are difficult to access by existing technologies. 
     In one aspect, provided herein is a method for targeted replacement of a genomic nucleic acid sequence of a cell, the method comprising: (A) introducing to the cell a polynucleotide sequence encoding a first protein complex comprising a targeted excision machinery for excising from the genome of the cell a nucleic acid sequence comprising one or more mutations; and (B) a recombinant mRNA encoding a second protein complex, wherein the recombinant mRNA comprises: (i) a nucleic acid sequence comprising the excised nucleic acid sequence in (A) that does not contain the one or more mutations, and (ii) a sequence encoding an L1 retrotransposon ORF2 protein under the influence of an independent promoter. 
     In one embodiment, the first protein complex may be an endonuclease complex independent of the second protein complex. In one embodiment, the first protein complex comprises a CRISPR-CAS system that uses sequence guided genomic DNA excision. In one embodiment, the methods described herein couples a CRISPR CAS system or any other gene editing system with a L1 transposon machinery (e.g., the second protein complex) that delivers a replacement gene with a payload capacity of greater than 4 kb, or 5 kb, or 6 kb, or 7 kb, or 8 kb or 9 kb or 10 kb. This coupling can be utilized in precisely excising a large fragment (a mutated gene causing a disease) from the genomic locus and integrating a large fragment of a gene or an entire gene that encodes a correct, non-mutated sequence. 
     A large number of genetic diseases may require delivery of gene delivery of large payloads, often exceeding the functional capacity of existing methods. Contemplated herein are methods and compositions disclosed herein that can be instrumental in further designing therapy for such diseases using retrotransposons. An exemplary list of genetic diseases include but are not limited to the ones listed in Table 10. 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 List of potential gene therapy applications 
               
            
           
           
               
               
               
               
               
            
               
                 Disease 
                 Gene 
                 CDS 
                 Expression 
                 Prevalence 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Stargardt 
                 ABCA4 
                 6.8 
                 kb 
                 Rod and Cone 
                 1:8000   
               
               
                   
                   
                   
                   
                 PRs 
               
               
                 Usher 1B 
                 MY07A 
                 6.7 
                 kb 
                 RPE and PRs 
                 3.2:100,000     
               
               
                 LCA10 
                 CEP290 
                 7.4 
                 kb 
                 PR (pan 
                 1:50,000  
               
               
                   
                   
                   
                   
                 retinal) 
               
               
                 USH1D, 
                 CDH23 
                 10.1 
                 kb 
                 PR 
                 3:100,000 
               
               
                 DFNB12 
               
               
                 RP 
                 EYS 
                 9.4 
                 kb 
                 PR ECM 
                 1:50,000  
               
               
                 USH2A 
                 USH2a 
                 15.6 
                 kb 
                 Rod and Cone 
                 4:100,000 
               
               
                   
                   
                   
                   
                 PRs 
               
               
                 USH2C 
                 GPR98 
                 18.0 
                 kb 
                 Mainly PRs 
                 1:100,000 
               
               
                 Alstrom 
                 ALMS1 
                 12.5 
                 kb 
                 Rod and Cone 
                     1:1,000,000 
               
               
                 syndrome 
                   
                   
                   
                 PRs 
               
               
                 Glycogen 
                 GDE 
                 4.6 
                 kb 
                 Muscle, Liver 
                 1:8000   
               
               
                 storage 
               
               
                 disease III 
               
               
                 Non-syndromic 
                 OTOF 
                 6.0 
                 kb 
                 Ear 
                 14:100,000  
               
               
                 deafness 
               
               
                 Hemophilia A 
                 F8 
                 7.1 
                 kb 
                 Liver 
                 1:10,000  
               
               
                 Leber congenital 
                 CEP290 
                 7.5 
                 kb 
                 Retina 
                 5:100,000 
               
               
                 aumaurosis 
               
               
                   
               
            
           
         
       
     
     Provided herein is a method for targeted replacement of a genomic nucleic acid sequence in a cell. In one embodiment, the method comprises: (A) excising from the genome of the cell a nucleic acid sequence comprising one or more mutations and (B) introducing into the cell a recombinant mRNA encoding: (i) a nucleic acid sequence comprising a wild type sequence relative to the sequence excised in (A) that does not contain the one or more mutation, (ii) a sequence encoding an L1 retrotransposon ORF2 protein under the influence of an independent promoter. In one embodiment, Step (A) further comprises introducing a short sequence comprising at least a plurality of adenylate residues at the excision site. In one embodiment, the In one embodiment, the nucleic acid sequence comprising a wild type sequence is operably linked with the ORF2 encoding sequence in a way such that the ORF2 reverse transcriptase integrates the sequence comprising the wild type non-mutated sequence into the genome. 
     In one embodiment, the cell is a lymphocyte. 
     In one embodiment, the cell is an epithelial cell. In some embodiments the cell is a retinal pigmented epithelial cell (RPE). 
     In one embodiment, the cell is a neuron. 
     In one embodiment, the cell is a myeloid cell. 
     In one embodiment, the cell is a stem cell. 
     In one embodiment, the cell is a cancer cell. 
     In one embodiment, the gene is selected from a group consisting of ABCA4, MY07A, CEP290, CDH23, EYS, USH2a, GPR98, ALMS1, GDE, OTOF and F8. 
     In one embodiment, the mRNA comprises a sequence for an inducible promoter. 
     In one embodiment, the expression of the nucleic acid sequence comprising a non-mutated sequence is detectable at least 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 or 35 days post infection. 
     In one embodiment, the method comprises introducing into the cell a recombinant mRNA in vivo. 
     In one embodiment, the method comprises introducing into the cell a recombinant mRNA ex vivo. 
     Provided herein is a method of treating a genetic disease in a subject in need thereof, comprising: introducing into the subject a composition comprising a polycistronic mRNA encoding a gene or fragment thereof, operably linked to a sequence encoding an L1 retrotransposon; wherein the gene or the fragment thereof is at least 10.1 kb in length. 
     In one embodiment, the method comprises directly introducing the mRNA systemically. 
     In one embodiment, the method comprises directly introducing the mRNA locally. 
     In one embodiment, the genetic disease is a retinal disease. For example, the disease is macular dystrophy. In one embodiment, the disease is Stargardt disease, also known as juvenile macular degeneration, or fundus flavimaculatus. The disease causes progressive degeneration and damage of the macula. The condition has a genetic basis due to mutation in the ATP-binding cassette (ABC) transporter gene, (ABCA4) gene, and arises from the deposition of lipofuscin-like substance in the retinal pigmented epithelium (RPE) with secondary photoreceptor cell death. In some embodiments, the method comprises direct delivery of the mRNA to the retina. 
     In one embodiment, the method comprises treating a nonsyndromic autosomal recessive deafness (DFNB12) and deafness associated with retinitis pigmentosa and vestibular dysfunction (USHID). In one embodiment, provided herein is a method of treating non-syndromic deafness (DFNB12) or Usher syndrome (USH1D), the method comprises introducing an mRNA comprising a copy of CDH23 or a fragment thereof operably linked to a sequence encoding an L1 retrotransposon. 
     Cell Specific Expression of Exogenous Polypeptide 
     Stable expression of an exogenous polypeptide may be accomplished in a variety of cell types (e.g. target cell types) using a mobile genetic element to target integration of a polynucleotide sequence (e.g. often referred to herein as an insert sequence in a construct) in the genome of the cell (e.g. a target cell). In some embodiments, the target cell is a post-mitotic cell, e.g., a mammalian cardiomyocyte, or an RPE cell. In some embodiments, the mobile genetic element comprises a human LINE 1 sequence. In some embodiments, the mobile genetic element is a human LINE1 sequence. In some embodiments, the mobile genetic element comprises a sequence encoding a human L1 ORFp1 protein. In some embodiments, the mobile genetic element comprises a sequence encoding a human L1-ORFp2 protein. In some embodiments, the mobile genetic element comprises a sequence encoding a human ORFp1 and human ORFp2 polypeptides. In some embodiments, a polynucleotide sequence is introduced in a target cell, the polynucleotide comprises of a sequence encoding a mobile genetic element, and an insert sequence. In some embodiments, the mobile genetic element comprises a polypeptide that post-translationally promotes a stable integration of an insert sequence into the genome of the target cell. In some embodiments, the mobile genetic element comprises a polypeptide further configured for site-specific integration of a given insert sequence into the genome of the target cell, for example but not limited to the embodiments as is described elsewhere in the specification. 
     In some embodiments, the polynucleotide described herein is an mRNA. The mRNA may be bicistronic or polycistronic. As is exemplified by the working embodiments, the insert sequence and the sequence encoding the mobile genetic element can be in reverse orientation with respect to each other. The mobile genetic element may integrate the insert sequence via target-primed reverse transcription (TPRT). 
     In some embodiments, the mobile genetic element comprises the human L1 retrotransposon, or fragments thereof. 
     In some embodiments, the polynucleotide can be specifically targeted to a cell type. In some embodiments, the polynucleotide may be composed in a nanoparticle, wherein the nanoparticle comprises one or more targeting moieties known to one of skill in the art. 
     Provided herein is a method of stably integrating an insert sequence into genomic DNA of a target cell, the method comprising contacting a composition to the target cell, the composition comprising a polynucleic acid, wherein the polynucleic acid comprises: an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous polypeptide, and a mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA; stably integrating the insert sequence into the genomic DNA of the target cell; and expressing an exogenous polypeptide in the target cell, wherein the target cell is a human hepatocyte. In some embodiments polynucleic acid is mRNA. In some embodiments, the mRNA is encapsulated in a nanoparticle for intracellular delivery. In some embodiments, the nanoparticle comprises a lipid. In some embodiments, the polynucleic acid is introduced into the hepatocyte by contacting a composition, comprising, for example, the polynucleic acid and a delivery vehicle (e.g., a nanoparticle comprises a lipid) to the hepatocyte such that it results in the successful uptake of the polynucleic acid by the hepatocyte. In some embodiments, the incorporation is via electroporation. In some embodiments, the polynucleic acid composition is electroporated in the hepatocyte. In some embodiments, the hepatocyte is electroporated under conditions suitable for expression of the polynucleic acid-encoded polypeptides and conducive to the viability of the hepatocyte. In some embodiments the integration of the insert into the genome of the hepatocyte is verified after incorporation the polynucleic acid by methods known to one of skill in the art, e.g., by genome sequencing. In some embodiments, the expression of the polypeptide from integrated insert sequence is verified at a suitable interval following incorporation of the polynucleic acid in the hepatocyte, wherein the suitable interval is about 4, 6, 8, 10, 12, 24 or 48 hours. In some embodiments, following electroporation of a population of cells comprising hepatocytes, the population of cells comprising the hepatocytes that have been subjected to the electroporation is cultured under conditions suitable for a hepatocyte for at least about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 8 hours, about 10 hours or about 24 hours. In some embodiments, the hepatocyte is cultured for about 48 hours, 72 hours, 96 hours or more under conditions suitable for a hepatocyte for growth. In some embodiments, the expression of a polypeptide encoded by the polynucleotide (e.g., the insert) is verified after 48, 72, or 96 hours, or after 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days or more in culture. In some embodiments, at least 2% of the human hepatocytes express the exogenous polypeptide at day 10 after incorporating. In some embodiments, about 2%, or about 5%, or about 10% or more of the human hepatocytes in the population of cells subjected to the electroporation express the exogenous polypeptide at day 10 after incorporating. 
     Provided herein is a method of stably integrating an insert sequence into genomic DNA of a target cell, the method comprising contacting a composition to the target cell, the composition comprising a polynucleic acid, wherein the polynucleic acid comprises: an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous polypeptide, and a mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA; stably integrating the insert sequence into the genomic DNA of the target cell; and expressing an exogenous polypeptide in the target cell, wherein the target cell is a human cardiomyocyte. In some embodiments polynucleic acid is mRNA. In some embodiments, the mRNA is encapsulated in a nanoparticle for intracellular delivery. In some embodiments, the nanoparticle comprises a lipid. In some embodiments, the polynucleic acid is introduced into the cardiomyocyte by contacting a composition, comprising, for example, the polynucleic acid and a delivery vehicle (e.g., a nanoparticle comprises a lipid) to the cardiomyocyte such that it results in the successful uptake of the polynucleic acid by the cardiomyocyte. In some embodiments, the incorporation is via electroporation. In some embodiments, the polynucleic acid composition is electroporated in the cardiomyocyte. In some embodiments, the cardiomyocyte is electroporated under conditions suitable for expression of the polynucleic acid-encoded polypeptides and conducive to the viability of the cardiomyocyte. In some embodiments the integration of the insert into the genome of the cardiomyocyte is verified after incorporation the polynucleic acid by methods known to one of skill in the art, e.g., by genome sequencing. In some embodiments, the expression of the polypeptide from integrated insert sequence is verified at a suitable interval following incorporation of the polynucleic acid in the cardiomyocyte, wherein the suitable interval is about 4, 6, 8, 10, 12, 24 or 48 hours. In some embodiments, following electroporation of a population of cells comprising cardiomyocytes, the population of cells comprising the cardiomyocytes that have been subjected to the electroporation is cultured under conditions suitable for a cardiomyocyte for at least about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 8 hours, about 10 hours or about 24 hours. In some embodiments, the cardiomyocyte is cultured for about 48 hours, 72 hours, 96 hours or more under conditions suitable for a cardiomyocyte for growth. In some embodiments, the expression of a polypeptide encoded by the polynucleotide (e.g., the insert) is verified after 48, 72, or 96 hours, or after 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days or more in culture. In some embodiments, at least 2% of the human cardiomyocytes express the exogenous polypeptide at day 10 after incorporating. In some embodiments, about 2%, or about 5%, or about 10% or more of the human cardiomyocytes in the population of cells subjected to the electroporation express the exogenous polypeptide at day 10 after incorporating. Provided herein is a method of stably integrating an insert sequence into genomic DNA of a target cell, the method comprising contacting a composition to the target cell, the composition comprising a polynucleic acid, wherein the polynucleic acid comprises: an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous polypeptide, and a mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA; stably integrating the insert sequence into the genomic DNA of the target cell; and expressing an exogenous polypeptide in the target cell, wherein the target cell is a human retinal pigment epithelial cell (RPE). In some embodiments polynucleic acid is mRNA. In some embodiments, the mRNA is encapsulated in a nanoparticle for intracellular delivery. In some embodiments, the nanoparticle comprises a lipid. In some embodiments, the polynucleic acid is introduced into the RPE by contacting a composition, comprising, for example, the polynucleic acid and a delivery vehicle (e.g., a nanoparticle comprises a lipid) to the RPE such that it results in the successful uptake of the polynucleic acid by the RPE. In some embodiments, the incorporation is via electroporation. In some embodiments, the polynucleic acid composition is electroporated in the RPE. In some embodiments, the RPE is electroporated under conditions suitable for expression of the polynucleic acid-encoded polypeptides and conducive to the viability of the RPE. In some embodiments the integration of the insert into the genome of the RPE is verified after incorporation the polynucleic acid by methods known to one of skill in the art, e.g., by genome sequencing. In some embodiments, the expression of the polypeptide from integrated insert sequence is verified at a suitable interval following incorporation of the polynucleic acid in the RPE, wherein the suitable interval is about 4, 6, 8, 10, 12, 24 or 48 hours. In some embodiments, following electroporation of a population of cells comprising RPEs, the population of cells comprising the RPEs that have been subjected to the electroporation is cultured under conditions suitable for a RPE for at least about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 8 hours, about 10 hours or about 24 hours. In some embodiments, the RPE is cultured for about 48 hours, 72 hours, 96 hours or more under conditions suitable for a RPE for growth. In some embodiments, the expression of a polypeptide encoded by the polynucleotide (e.g., the insert) is verified after 48, 72, or 96 hours, or after 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days or more in culture. In some embodiments, at least 2% of the human RPEs express the exogenous polypeptide at day 10 after incorporating. In some embodiments, about 2%, or about 5%, or about 10% or more of the human RPEs in the population of cells subjected to the electroporation express the exogenous polypeptide at day 10 after incorporating. 
     Provided herein is a method of stably integrating an insert sequence into genomic DNA of a target cell, the method comprising contacting a composition to the target cell, the composition comprising a polynucleic acid, wherein the polynucleic acid comprises: an insert sequence, wherein the insert sequence comprises a sequence that is a reverse complement of a sequence encoding an exogenous polypeptide, and a mobile genetic element comprising a sequence encoding a polypeptide, wherein the polypeptide encoded by the sequence of the mobile genetic element promotes integration of the insert sequence into genomic DNA; stably integrating the insert sequence into the genomic DNA of the target cell; and expressing an exogenous polypeptide in the target cell, wherein the target cell is a human neuronal cell. In some embodiments polynucleic acid is mRNA. In some embodiments, the mRNA is encapsulated in a nanoparticle for intracellular delivery. In some embodiments, the nanoparticle comprises a lipid. In some embodiments, the polynucleic acid is introduced into the neuronal cell by contacting a composition, comprising, for example, the polynucleic acid and a delivery vehicle (e.g., a nanoparticle comprises a lipid) to the neuronal cell such that it results in the successful uptake of the polynucleic acid by the neuronal cell. In some embodiments, the incorporation is via electroporation. In some embodiments, the polynucleic acid composition is electroporated in the neuronal cell. In some embodiments, the neuronal cell is electroporated under conditions suitable for expression of the polynucleic acid-encoded polypeptides and conducive to the viability of the neuronal cell. In some embodiments the integration of the insert into the genome of the neuronal cell is verified after incorporation the polynucleic acid by methods known to one of skill in the art, e.g., by genome sequencing. In some embodiments, the expression of the polypeptide from integrated insert sequence is verified at a suitable interval following incorporation of the polynucleic acid in the neuronal cell, wherein the suitable interval is about 4, 6, 8, 10, 12, 24 or 48 hours. In some embodiments, following electroporation of a population of cells comprising neuronal cells, the population of cells comprising the neuronal cells that have been subjected to the electroporation is cultured under conditions suitable for a neuronal cell for at least about 2 hours, about 3 hours, about 4 hours, about 5 hours, about 6 hours, about 8 hours, about 10 hours or about 24 hours. In some embodiments, the neuronal cell is cultured for about 48 hours, 72 hours, 96 hours or more under conditions suitable for a neuronal cell for growth. In some embodiments, the expression of a polypeptide encoded by the polynucleotide (e.g., the insert) is verified after 48, 72, or 96 hours, or after 4 days, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days or more in culture. In some embodiments, at least 2% of the human neuronal cells express the exogenous polypeptide at day 10 after incorporating. In some embodiments, about 2%, or about 5%, or about 10% or more of the human neuronal cells in the population of cells subjected to the electroporation express the exogenous polypeptide at day 10 after incorporating. 
     EXAMPLES 
     It should be appreciated that the invention should not be construed to be limited to the examples which are now described; rather, the invention should be construed to include any and all applications provided herein and all equivalent variations within the skill of the ordinary artisan. 
     Example 1. Exemplary Retrotransposon Designs Constructs 
     Provided here are exemplary strategies of designing retrotransposon constructs for incorporating into the genome of a cell and expressing an exemplary transgene.  FIG.  1 B  and  FIG.  1 C  illustrates various strategic designs for integrating an mRNA encoding transgene into the genome of a cell. GFP shown here in a box is an exemplary transgene. The mRNA encoding the transgene (e.g., GFP) can be co-expressed with a nucleic acid sequence encoding an ORF2p protein, in either sense or antisense orientation; the respective coding sequences may be in a monocistronic or bicistronic construct shown under exemplary Cis-strategies ( FIG.  1 B  and  FIG.  1 C ). CMV/T7 are promoters. 
     On the other hand, the same could be directed to be expressed in a trans manner. The trans-strategy can include a sequence encoding an ORF2p protein or both ORF1p and ORF2p proteins from a bicistronic sequence and an mRNA encoding a GFP in a sense or antisense direction in the 3′UTR of any gene. The transgene is flanked by a retrotransposing sequence comprising transposase binding sequences, an A-box and B-box, and a poly A tail.  FIG.  2 A  illustrates three exemplary designs for expressing an exemplary transgene GFP by stably incorporating the sequence encoding GFP using the constructs. The first construct comprises a sequence encoding GFP, flanked by L1 5′-UTR; and a poly A sequence at the 3′ UTR, in absence of any transposase binding elements. The second and the third constructs comprise a sequence encoding GFP, a 3′UTR an A Box and a B-box, and a poly A sequence at the 3′ UTR. The third construct comprises an additional sequence encoding ORF2p. Expected GFP expression levels at 72 hours are shown on the right side.  FIG.  2 B  illustrates three exemplary designs for expressing an exemplary transgene GFP in an mRNA that either encodes RFP or ORF2p by stably incorporating the sequence encoding GFP using the constructs. The first construct comprises a sequence encoding RFP, and a poly A sequence at the 3′ UTR, in absence of any L1 elements. The second and the third constructs comprise a 3′UTR comprising an A Box and a B-box, and a poly A sequence at the 3′ UTR. The second construct comprises a sequence encoding RFP and the third construct comprises a sequence encoding ORF2p. Expected RFP and GFP expression levels at 72 hours are shown on the right side. 
     Example 2. Exemplary circRNA Designs Constructs 
     In this example, modular designs for circRNA are demonstrated, which incorporate a stretch of about 50 nucleotide long RNA having naturally occurring tertiary structures in order to prepare a circRNA. Use of the tertiary-structure forming RNA makes the circRNA formation process independent of sequence mediated hybridization for circularization. These RNA motifs having tertiary structures can be incorporated in the desired RNA having an exon and an intron in place of the 5′ and 3′ homology arms, thereby forming the terminal RNA scaffolds for circularization. 
     TectoRNA: RNA-RNA binding interfaces are constructed by combining pairs of GNRA loop/loop-receptor interaction motifs, yielding high affinity, high specificity tertiary structures. ( FIG.  3 B ). Pairs of GNRA loop/loop-receptor interaction motifs are fused using the four-way junction from the hairpin ribozyme to create divalent, self-assembling scaffolding units (‘tectoRNA’) which help form a closed cooperatively assembling ring-shaped complexes. Using two orthogonal loop/loop-receptor interaction motifs, RNA monomers are designed that are capable of directional assembly in either the parallel (‘up-up’) or anti-parallel (‘up-down’) assembly modes. In anti-parallel assembly of interacting molecules, each incorporated monomer switches the directionality of the growing chain and thus compensates for its intrinsic bending, producing long, relatively straight multi-unit chains. For selecting a tectoRNA scaffolds having minimum occurrences of alternative secondary structures, sequences are checked by submitting them to the RNA folding program Mfold (bioinfo.math.rpi.edu/˜zukerm/rna/mfold) which predicts the thermodynamically favored secondary structure of a given RNA sequence. A thermodynamically favored structure is selected for scaffolding that has minimum alternative secondary structures (typically but not exclusively, no other secondary structure is closer than 15% in energy to the lowest energy structure). RNA molecule is prepared by conventional methods, such as in vitro run-off transcription using T7 RNA polymerase.  FIG.  3 B  shows a RL-GAAA loop structure. In order to profile tectoRNA heterodimers a fluorescence-based chip-flow piece testing method is utilized. In this method, a library of potential variants of the structured RNA (chip piece) is synthesized as DNA templates and amplified to include sequencing adapters and regions for RNAP initiation. Each DNA variant is transcribed in situ into RNA, enabling display of sequence-identified clusters of RNA on the surface of the sequencing chip. The fluorescently-labeled tectoRNA binding partner, the “flow piece”, is introduced to the sequencing chip flow cell at increasing concentrations, allowing quantification of bound fluorescence to each cluster of RNA after equilibration. These fluorescence values are used to derive the affinity of the flow piece to each chip piece variant ( FIG.  3 C ), in terms of the dissociation constant (K d ) and binding free energy, (ΔG=RT log(K d )). 
     The selected terminal RNA scaffold segments comprising the tertiary structures are incorporated using T7 transcription or ligated at the 5′ and 3′ ends of the desired RNA to be circularized; or are incorporated in the desired RNA by any known molecular biology techniques. 
     Example 3: Exemplary Retrotransposon Designs with Enhanced Specificity 
     In this example, designs for a nucleic acid construct for L1-mediated retrotransposon for enhanced target specificity is demonstrated. An mRNA is designed comprising ORF2 encoding sequence and a sequence encoding a gene of interest, to incorporate the gene of interest into the genome of a cell using ORF2. In one exemplary design, the construct comprises an ORF2 that is further modified. 
     As shown in  FIG.  4 A , ORF2 protein initiates retrotransposition by binding to its own poly A sequence. However, because poly A is abundantly present in mRNAs, a non-specific binding and integration becomes a possibility. To increase the specificity, a recombinant ORF2 is designed comprising an mRNA-binding domain of a heterologous protein, and the cognate mRNA sequence for the heterologous mRNA-binding domain is inserted near the poly A sequence in the 3′-UTR and the ORF2 poly A binding site. 
     A chimeric ORF2 is thereby generated as shown in ( FIG.  4 B ), in which a high affinity RNA-binding domain of a heterologous protein encoding sequence is incorporated or fused to the ORF2 sequence and cognate RNA sequences corresponding to the high affinity RNA-binding protein is incorporated in the 3′UTR region of the mRNA, proximal to the poly A region. In this example the heterologous high affinity RNA-binding domain is derived from MCP coat protein MS2 (shown as M in the figure), is incorporated within the ORF2 sequence and the cognate sequence, the MS2 hairpin, is included in the 3′ UTR sequence of the mRNA ( FIG.  4 B ). The MS2 binds to the cognate sequence, increasing the specificity of the chimeric ORF2 to its own mRNA for reverse transcribing and incorporating the respective sequence associated with the ORF2 mRNA in the mammalian cell genome ( FIG.  4 B ). 
     In other exemplary designs, attempts to increase specificity of integration of the transgene by the ORF2 within the genome of a target cell is undertaken. In one exemplary design, Mega TAL encoding sequence fused to an ORF2 as shown in  FIG.  4 C  (upper panel). Along with that, the ORF2 is mutated to remove its ability to recognize and bind to RNA sequence that has less specificity. The fused protein is directed to the TAL binding sequence incorporated within the 3′UTR and perform endonuclease function. The Mega TAL DNA binding sequence is targeted by the fusion protein. Likewise, other chimera ( FIG.  4 C  (middle panel)) and fusion protein with a specific DNA binding domain  FIG.  4 C  (lower panel) are designed. 
     Example 4. Exemplary Plasmid Design and Developments for LINE-1 Mediated Retrotransposition of an Exogenous Nucleic Acid Sequence 
     In this example plasmid vectors are generated for delivery and incorporation of a recombinant LINE-1 construct comprising an ORF2 transposon element operably linked to a transgene transposable into a mammalian cell, and regulatory elements for mRNA transcription and stabilization. The mRNA can be transcribed in a bacterial host cell, which can be further processed and/or purified for introduction into a mammalian cell in vitro or administration in an organism, such as a mammal, a rodent, sheep, pig or a human. 
     Any suitable vector backbone is used for incorporating the recombinant nucleic acid sequence as insert and transcribing in a bacterial system for mRNA generation; or in vitro transcription system may be utilized to generate an mRNA comprising the recombinant nucleic acid sequence. Several features are added to the plasmid. Upon successful scalable mRNA production, and purification, the mRNA may be introduced in a mammalian cell of interest, such as a myeloid cell. 
     Plasmids traditionally used in the field of study for retrotransposition lack designer genes, gene blocks, and Gibson assembly methods were used regularly to insert different features. A new vector that takes features from the old vectors but has flexibility to insert new features can be beneficial both for the study and optimization of LINE-1 elements as a gene delivery system. Below is an outline of base features and additional features that can increase retrotransposition frequency, both using the plasmid alone or the mRNA transcribed from the plasmid. In an exemplary plasmid design shown graphically in  FIG.  5 (I) , which contains the natural LINE-1 sequence with the original 5′UTR, 3′UTR and interORF sequence with no restriction sites to swap out any of these features. New optimized plasmid:
         Removed Dox inducible promoter, replaced with CMV or EIF1a or EF1a promoter   Added a T7 site to make mRNA   Codon optimized ORF1 and ORF2   Added a WPRE element to stabilize mRNA   Added FLAG tag to ORF2 to help with protein detection   Decreased size from 18 kb to 14 kb   Added blunt restriction sites (dotted lines with blunt arrows) at each feature to facilitate insertions   Includes a G418 selection marker       

     The plasmid is shown in  FIG.  5    (II). 
     With Gibson a reverse split GFP is inserted for plasmid reporter gene as shown in  FIG.  5    (III). A complete reverse GFP for the mRNA reporter is inserted as in  FIG.  5    (IV). 
     Using the plasmid construct in  FIG.  5    (V) as parent, a nuclear localization sequence (NLS) is inserted at the N terminus of ORF2 to help with nuclear import ( FIG.  5    (VI)). An IRES or another termination/promoter sequence is inserted to increase expression of ORF2 ( FIG.  5    (VII)). To facilitate stronger interactions between ORF2 and the mRNA, MS2 hairpins are inserted in the 3′UTR and a MS2 coat protein sequence in the N terminus of the ORF2 protein ( FIG.  5    (VIII)). A corresponding exemplary ORF2 with enhanced specificity and its mechanism of action is disclosed in the preceding example and in  FIG.  4 B . To facilitate stronger interactions of the mRNA with the translating ribosome and to stall translation so that nascent ORF2 will more likely bind the mRNA, an Alu element is inserted in the 3′UTR of the mRNA ( FIG.  5    (IX)). To potentially use a more active ORF2 protein, the ORF2&#39;s RT domain is replaced with the Group II intron&#39;s reverse transcriptase domain ( FIG.  5    (X)). Additionally, the minke whale genome has the highest number and percentage of active LINE elements (˜5,000 with 60% active compared to humans that have 480 with 3.6% active). The two sequences are 67% identical and the whale sequence has the active endonuclease and reverse-transcriptase residues. The respective minke whale domains can be used to replace native ORF2 endonuclease and/or RT domains or design a chimera domain. 
     Example 5. mRNA Design Synthetic mRNA Generation 
     mRNA can be strategically designed for synthetic production by oligosynthesis and or ligation of oligonucleotides. Additionally, such designs are useful for in vitro transcription (IVT) mediated mRNA generation. The mRNA strategy can include the same variants as the plasmid strategy discussed in the previous example. The main differences are that the reporter GFP sequence does not include an intron ( FIG.  6 A ) and that the constructs can be delivered without the ORF1 coding region ( FIG.  6 B ). 
     Example 6. Structural Features for Increased mRNA Half-Life 
     In this example, structural features are introduced in the mRNA comprising the retrotransposition elements and/or the transgene for increasing the mRNA half-life. The goal is to increase the duration of protein expression from the mRNA in primary monocytes from three days to at least 5 days with an ultimate goal of 10 days. 
     As shown in  FIG.  7 B  (left), the mRNA comprising a sequence encoding the transgene when introduced into a CD14+ myeloid cell (monocyte), is translated and expresses a chimeric receptor (an ATAK construct) capable of binding to an antigen on a cancer cell. 
     A number of mRNA designs are generated by synthesizing various gene blocks comprising singly, or combinations of one or more of: (i) a G-quadruplex, (ii) a viral pseudoknot structure in the 5′ UTR; and/or (iii) one or (iv) more xrRNA loop structures in the 3′ UTR (v) a triplex RNA structure as shown in  FIG.  7 A ; and cloned into the transcription vector at the respective UTRs adjoining the coding sequence of the transgene. These constructs are individually prepared by an off-site vendor and tested in-house for determining stability of the mRNA, as measured by the expression of the chimeric receptor (An exemplary receptor and its function is depicted graphically in  FIG.  7 B  (left). The process flow chart is shown on  FIG.  7 B  (right). In short, constructs are cloned into plasmids, with encoded or modified poly A tails. The mRNA was transcribed and purified. Meanwhile, frozen monocytes are thawed and harvested. Harvested cells were electroporated with the purified mRNA (5-10 ug), and cultured for 1, 2, 3, 5 days. Cells positive for the chimeric receptor (binder positive cells), are detected by means of their ability to bind to a target cell or a substrate coated with the target antigen. The expected results are shown in  FIG.  7 C . Bulk or purified mRNA expressing one or more of the structural features outlined in (i)-(v) (data denoted by solid squares) or a combination thereof outperforms the commercially available counterparts that do not contain any of the features outlined in (i)-(v) (data denoted by triangles). 
     Example 7. LINE-1 Retrotransposon Plasmid Mediated Delivery of GFP Gene 
     In this test run, genomic integration of a GFP cargo and expression the GFP protein using a LINE-1 retrotransposon system was verified. The LINE-I-GFP construct (LINE-1 plasmid GFP) is exemplified in  FIG.  8 A : A plasmid construct having a LINE-1 sequence encoding ORF1p (ORF1), a sequence encoding ORF2p (ORF2), and a CMV promoter driven split GFP gene situated in the 3′UTR of the LINE-1 in reverse orientation with respect to the ORFs. The split GFP is designed to have an intronic sequence inserted in between a splice donor and acceptor sites, which ensures that the GFP is expressed only after integration and splicing mediated removal of the noncoding sequence in the middle of the coding sequence. In this case the cargo is 2.1 kb. HEK293T cells were transfected with the plasmid using Fugene reagent, and plasmid positive cells were selected by puromycin. The mRNA generated from a genome integrated GFP successfully translates and is measured by flow cytometry, as indicated as change in mean fluorescence intensity (MFI) ( FIG.  8 B ) and fraction of cells with GFP fluorescence intensity compared to mock transfected cells ( FIG.  8 C ). Mock transfected cells received the plasmid that lack the GFP sequence. 
     Example 8. LINE-1 Retrotransposon Plasmid Mediated Delivery of a Chimeric Receptor Gene 
     This example demonstrates that a recombinant gene can be successfully expressed using the LINE-1 sequence in a cell. HEK 293 cells were transfected with a plasmid having the LINE-1 elements, with a 3 kb cargo sequence encoding recombinant receptor protein CD5-intron-fcr-PI3K (ATAK) that is interrupted by an intron sequence in the CD5 binding domain. The cargo is a chimeric receptor that has a CD5 binding extracellular domain, a FCRγ transmembrane domain, and an intracellular domain having a PI3-kinase recruitment domain. The schematic representation of the retrotransposon plasmid is shown in  FIG.  9 A . As in the design of the experiment above, the ATAK receptor cannot express unless it is integrated in the genome and the intron is spliced off. Following transfection in HEK293T cells, the receptor expression is detected using labeled CD5 as bait for the CD5 binding extracellular domain. Results shown in  FIGS.  9 B and  9 C  show successful integration and expression of the receptor. 36.5% cells were ATAK (CD5 binder) positive ( FIG.  9 C ). 
     In a further modification, a LINE-1 construct (LINE-1plasmid-cd5_fcr-pi3k_t2a_GFPintron) with a longer 3.7 kb cargo sequence encoding a non-interrupted recombinant receptor protein CD5-intron-fcr-PI3K and an interrupted GFP sequence with a T2A sequence between receptor and the GFP sequences ( FIG.  10 A ). Normalized against mock-transfected cells, there was a greater than 10-fold increase of the ATAK receptor and GFP double-positive cells was noted ( FIG.  10 B ). Exemplary fluorescence identification of GFP and fluorescent tagged CD5 binding and gating quantitation for experimental runs are shown in  FIG.  10 C  and  FIG.  10 D . 
     Example 9. mRNA Encoding LINE-1 Retrotransposon for Delivery of a Cargo Gene 
     In this assay, capability of delivering and expressing a LINE-1 retrotransposable gene sequence as an mRNA was tested. An mRNA encoding an ORF1 (ORF1-FLAG-mRNA), and an mRNA encoding ORF2 and GFP in the antisense direction with a CMV promoter sequence (ORF2-FLAG-GFPai) are designed as shown in  FIG.  1 A . The cargo size in this assay was 2.4 kb, and GFP is in antisense orientation with respect to ORF2 sequence. The mRNAs were electroporated in 293T cells and the reporter genes expression was demonstrated as shown in  FIG.  11 B . This experimental set up demonstrated that no ORF1-readthrough is necessary for the expression of the ORF2p, and expression of ORF2p from a different mRNA molecule can allow higher expression of ORF2p and GFP. With these results, a successful delivery of the LINE-1 and cargo in the form of mRNA was achieved. 
     In order to determine whether the relative levels of ORF1 and ORF2 mRNA affected GFP expression an experiment was set up to test the varying amounts of ORF1 and ORF2 mRNAs ( FIG.  11 A ). 3× the amount of each and together is tested for increases in GFP+ cells and results are shown in  FIG.  12 A . Fold increase is relative to 1×ORF2-GFP and 1×ORF1 mRNA. GFP expression was higher when 3×ORF1 was used with 1×ORF2, but not the reverse; whereas having both 3×ORF1 and 3×ORF2 showed the maximum level of GFP expression in the sets compared. The cargo size here is 2.4 kb.  FIG.  12 B  shows fluorescent microscopy image of GFP+ cells following retrotransposon mRNA electroporation. 
     A complete LINE-1 mRNA encoding both ORF1 and ORF2 and GFP transgene in antisense orientation in a single mRNA molecule (LINE 1-GFP mRNA construct) was tested for delivery and genomic integration in a cell. mRNA contains the bicistronic ORF1 and ORF2 sequence with a CMV-GFP sequence in the 3′UTR going from 3′-5′ ( FIG.  13 A ). In this study the cargo size is 2.4 kb. As shown in  FIG.  13 B , upon retrotransposition of the delivered ORF2-cmv-GFP antisense (LINE-1 mRNA), third bar from left, cells expressed higher GFP compared to ORF1 and ORF2 being on separate mRNA molecules (graph bar 1, 2). Inclusion of ORF1 in a separate mRNA in addition to LINE-1 complete mRNA increased GFP expression over LINE-1 alone. Inclusion of ORF2+GFP expectantly showed higher GFP which could be the contribution of the additional ORF2 with the GFP cargo encoding mRNA. 
     To test whether subsequent electroporation increases retrotransposition efficiency, cells were electroporation every 48 hours. GFP positive cells were assessed using flow after culturing for 24-72 hrs. The fluorescence data were normalized to the values in the set with a single electroporation event. As shown in  FIGS.  14 A and  14 B , multiple electroporation led to an upward trend in the expression of the transposed gene, but the changes were modest. 
     Example 10. Modifications to the ORF2 Protein Sequence to Enhance Retrotransposition by mRNA 
     Modification of the LINE-1 sequence to enhance retrotransposition via mRNA delivery were tested using GFP reporter as readout. The experiment was performed as follows. All modifications were in the context of the bicistronic ORF1 and ORF2 sequence. (i) ORF2-NLS fusion was created by inserting C-terminal NLS sequence to the ORF2 sequence. (ii) Human ORF2 was replaced with Minke whale ORF2; (Ivancevic et al., 2016). (iii) Incorporation of an Alu element in the 3′UTR: Using a minimal sequence of the Alu element (AJL-H33Δ; Ahl et al., 2015) in the 3′UTR of the LINE-1. (iv) MS2-hairpin in the 3′UTR+ORF2-MCP fusion: MS2 hairpins in the 3′UTR of the LINE-1 sequence and a MS2 hairpin binding protein (MCP) fused to the ORF2 sequence ( FIG.  15 A ). The mock construct had the wild-type human ORF2 sequence. 
     Quantification of the fold increase in the fraction of GFP positive cells relative to mock construct electroporated cells are shown in  FIG.  15 B . 
     Example 11. Retrotransposition in an Immune Cell 
     In this experiment, the inter-ORF region is further manipulated to determine if any of the changes improve GFP expression after transfection of the HEK cells. Taking LINE-1plasmid GFP, the inter-ORF region is manipulated as follows: (a) In one construct the inter-ORF region is replaced with an IRES from CVB3; (b) In another construct, the inter-ORF region is replaced with an IRES from EV71; (c) In three separate constructs, an E2A or P2A or T2A self-cleavage sequence is intercalated in the inter-ORF region. Result are as shown in  FIG.  16   . Compared to the LINE-1 plasmid GFP (LINE-1 wild type plasmid) led to only modest changes in the GFP readout, especially with T2A sequence insertion. Insertion of EV71 IRES sequence improved GFP expression, while CVB3 IRES did not show any improvement. 
     Example 12. Retrotransposition in an Immune Cell 
     To test retrotransposition in immune cells, LINE-1 plasmid and mRNA were tested with the CMV-GFP antisense reporter cargo by electroporating into Jurkat cells, which is a T cell lymphoma line ( FIG.  17 A - FIG.  17 B ). Mock set were electroporated with a plasmid with no GFP sequence. GFP expression in the transfected cells was assessed, representative data at 4 days post electroporation is shown in  FIG.  17 B . Fold increase is reported relative to mock transfected cells. Both plasmid and mRNA delivery modes resulted in successful GFP expression. 
     Next, THP-1 cells (a myeloid, monocytic cell line) were electroporated with a plasmid having LINE-1 sequences and a 3.7 kb cargo encoding a chimeric HER-2 binding receptor, and a split GFP (LINE-1 plasmid Her2-Cd3z-T2A-GFPintron) ( FIG.  18 A ). The cargo is a chimeric receptor that comprises a HER2 binding extracellular domain, a CD3z transmembrane domain, and split GFP reporter. The plasmid was successfully integrated into the genome and showed prolonged expression, as demonstrated in  FIG.  18 B . Representative expression at day 6 post transfection is shown in the figure. From these studies, it was demonstrated that LINE-1 mediated gene delivery can result in successful stable genomic integration in various cell types, including epithelial cell types (HEK-293T cells); T cells (e.g., Jurkat cells); and cells of myeloid lineage (e.g., THP-1 cells) and results in prolonged expression. Moreover, unlike CRISPR dependent technologies such as Prime editing, retrotransposition can result in integration of large genetic cargo, and, these can be delivered as a single nucleic acid construct. 
     Example 13. External Methods for Further Enhancing Efficiency of LINE-1 Mediated Retrotransposition of the Cargo Sequences 
     In this section, methods for further enhancing the efficiency of retrotransposition of cargo sequences into the genome of cells are detailed. 
     Cell cycle synchronization by selection of cells in a population that are in a certain stage of cell cycle or G1 arrest by a suitable agent can lead to higher nucleic acid uptake efficiency, e.g., plasmid vector transfection efficiency or electroporation efficiency. In this assay, cells are pre-sorted and each group is separately electroporated to ensure uniform electroporation. The efficiencies of electroporation are compared between these groups and a cell cycle stage that results in highest efficiency as determined by the expression of the GFP test plasmid or mRNA is selected ( FIG.  19   ). 
     In another variation of this experiment, cells are synchronized with or without sorting by treating the cells, with a cell cycle arrest reagent for a few hours prior to electroporation. An exemplary list of cell cycle arrest reagents is provided in Table 1. The list is non-exhaustive, and is inclusive of reagents that can be proapoptotic, and hence careful selection suitable for the purpose and dose and time of incubation is optimized for use in the particular context. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Exemplary non-exhaustive list of small molecule 
               
               
                 reagents that are used for inhibiting cell cycle 
               
            
           
           
               
               
               
            
               
                 Agent 
                 Cell cycle 
                 Mechanism 
               
               
                   
               
               
                 5-[(4- 
                 Arrests cell cycle at 
                 Inhibits c-Myc-Max 
               
               
                 Ethylphenyl)methyl- 
                 G0-G1 
                 dimerization 
               
               
                 ene]-2-thioxo-4- 
               
               
                 thiazolidinone 
               
               
                 Itraconazole 
                 Inhibits cell cycle at 
                 SMO antagonist 
               
               
                   
                 G1 
               
               
                 ABT 751 (Tocris 
                 Blocks cell cycle at 
                 Inhibits microtubule 
               
               
                 Bioscience, cat 
                 G2M 
                 proliferation 
               
               
                 #4138) 
               
               
                 Artesunate 
                 Arrests cell cycle at 
                 Suppresses ROS- 
               
               
                   
                 G2M 
                 induced NLRP3 
               
               
                 AZD 5438 
                 Blocks cell cycle at 
                 Inhibits Cdk 
               
               
                   
                 G2M, M, S and G1 
               
               
                   
                 phases 
               
               
                 Baicalein 
                 Arrests cell cycle at 
                 Inhibits lipoxygenases 
               
               
                   
                 G1 and G2 phases 
               
               
                 CPI 203 (alternative 
                 Arrests cell cycle at 
                 BET bromodomain 
               
               
                 name: TEN 101) 
                 G1 phase 
                 inhibitor 
               
               
                 Diadzein 
                 Arrests cell cycle at G1 
                 Estrogen receptor agonist 
               
               
                 DIM 
                 Blocks cell cycle at 
                 Induces EGFR activation 
               
               
                   
                 G2M 
               
               
                 Epothilone B 
                 Arrests cell cycle at 
                 Inhibits tubulin 
               
               
                   
                 G2M 
                 proliferation 
               
               
                 Indirubin-3′-oxime 
                 Antiproliferative 
                 Inhibits GSK3b 
               
               
                 MPC 6827 
                 Cell cycle arrest 
                 Inhibits microtubule 
               
               
                 hydrochloride 
                   
                 proliferation 
               
               
                 Pladienolide 
                 Inhibits G1 and G2/M 
                 Decreases mRNA 
               
               
                   
                   
                 splicing 
               
               
                 Plumbagin 
                 Induces G2/M arrest 
                 Inhibits TOR signaling 
               
               
                   
                   
                 and others 
               
               
                 Temsirolimus 
                 Induces G1/S 
                 mTOR inhibitor 
               
               
                 Toceranib 
                 Cell cycle arrest 
                 Inhibits PDGFR and 
               
               
                   
                   
                 VEGFR 
               
               
                 WYE 687 
                 Induces G1 arrest 
                 mTOR inhibitor 
               
               
                 dihydrochloride 
               
               
                 YC1 
                 Induces G1 arrest 
                 Guanylyl cyclase 
               
               
                   
                   
                 activator 
               
               
                   
               
            
           
         
       
     
     For certain ex vivo usages, retrotransposition is enhanced by inducing DNA double stranded breaks (DSB) in a cell that expresses a retrotransposition machinery as described in any of the examples above by controlled irradiation, which create opportunities for the homologous recombination and priming for the reverse transcriptase ( FIG.  20   ). In another example, cells transfected with LINE-1 plasmid GFP construct and subjected to an irradiation pulse. GFP expression is monitored. The intensity and time of irradiation is optimized for obtaining the maximum benefit, as indicated by higher GFP expression. 
     In another example, cells transfected with LINE-1 plasmid GFP were divided into experimental sets that are treated as follows (i) irradiation in order to induce DSB (as described above); (ii) treat cells in this set with a small molecule, such as SCR7, that blocks DNA ligase and therefore inhibits the DNA damage repair machinery. Preventing protective repair mechanism from inhibiting the progress of the retrotransposition is expected to enhance GFP expression: (iii) irradiate the cells then treat the cells with SCR7, combination of the two is expected to show a more robust effect. GFP expression is monitored over a period of 6 days, and the set that shows maximum GFP fluorescence over the longest period indicates a condition that is adopted in further studies. 
     Example 14. Enhancing Efficiency of LINE-1 Mediated Retrotransposition of the Cargo Sequences by Further Modification of the Construct 
     Enhancing non-coding regions of the construct to offer stability and higher expression. In this example a LINE-1 plasmid-GFP is further modified to test for increased GFP expression as follows: (a) In one construct, the 5′UTR is replaced with an UTR of a complement gene; (b) In another construct, the 3′ UTR is replaced with the UTR sequence of B-globin gene for increased stability; (c) In another construct the inter-ORF region is replaced with an IRES from CVB3; (d) In another construct, the inter-ORF region is replaced with an IRES from EV71 (e) In three separate constructs, an E2A or P2A or T2A self-cleavage sequence is intercalated in the inter-ORF region as shown in a diagrammatic representation in  FIG.  21   . In addition to the above, various combinations of (a)-(e) and additional combinations listed in Table 2 are tested using the same set-up as above. GFP expressions are monitored after transfection of the constructs in parallel test sets into HEK293T cells to see if any of these constructs increased GFP expression compared to the LINE-1 plasmid GFP alone. The combinations that show improvement are adopted. 
                     TABLE 2                  Exemplary combinations of 5′ and 3′ UTR and       inter-ORF insertion elements for inclusion in the LINE-1       construct for increase in retrotransposition efficiency.                         5′-UTR sequences   3′-UTR   Inter-ORF       selected from sequences   sequences   sequences               Complement 5′UTR   WPRE   T2A, E2A, P2A       Covid-19 5′ leader sequence   B-globin 3′UTR   CVB3 IRES       CYBA 5′UTR   RSV RSE   EV71 IRES       CYP2E1 5′ UTR   AREs   EMCV IRES           RNA zipcodes for the   PV IRES           ER   CSFV IRES           mtRNR1-AES   HRV2 IRES               AAA (tri alanine               fusion or any               fusion-linker               sequence)                    
II. Enhancing localization and retention of the ORFs in the nucleus. In this example, LINE-1 plasmid-GFP is further modified to test for increased GFP expression as follows: (a) the ORF2 encoding sequence is fused with a nuclear localization sequence (NLS) (graphically represented in  FIG.  15 A  second construct from top). (b) the ORF1 encoding sequence is fused with a nuclear localization sequence (NLS), graphically represented in  FIG.  22   ; and (c) An Alu binding sequence is inserted 3′ of the sequence encoding ORF2 reverse transcriptase (graphically represented in  FIG.  15 A , fourth construct from the top; (d) Both (a) and (c) together (not shown); (e) Both (b) and (c) together, the NLS sequence is fused to the ORF1 N-terminus, and an Alu binding sequence is inserted 3′ of the sequence encoding ORF2 reverse transcriptase ( FIG.  22   ) and (f) Integrating a SINE-derived nuclear RNA LOcalizatIoN (SIRLOIN) sequence in LINE-1 3′ UTR. HEK-293T cells were transfected with constructs (a)-(f) and the LINE-plasmid GFP construct in parallel. GFP expression is monitored after transfection into HEK293T cells.
 
     The set that shows maximum GFP fluorescence over the longest period is adopted. 
     III. Modifying construct to increase LINE-1-protein-RNA complex binding to the ribosome. In this example, an additional sequence is inserted in the 3′UTR of the LINE-1 construct to increase association of the LINE-1 protein RNA construct to the ribosomes, the sequence is an Alu element, or a ribosome binding aptamer ( FIG.  23   ). 
     For enhancing LINE-1 protein-RNA complex binding to the ribosome, insertion of the following elements in the 3′ UTR of the mRNA is done and tested similar to the experiments above. Insertion of Alu elements is described above. In separate constructs, Alu element truncations, Ribosome binding aptamers (109.2-3) and Ribosome expansion segments (ES9S) binding sequence are inserted and each tested for increase in GFP expression. 
     IV. Enhancing binding of ORF2 to its own mRNA for retrotransposition. In this example, a sequence containing MS2 binding loop structure is introduced into the 3′UTR of the LINE-1, and a sequence encoding MS2 RNA binding domain is fused to the RNA binding domain of the ORF2p-RT (graphically represented in  FIGS.  4 A and  4 B , and  FIG.  24   , construct SEQ ID NO: 15). The fused protein will specifically attach to the MS2-binding structural motif in the 3′ UTR, and therefore any non-specific binding and retrotransposition is minimized ( FIG.  24   ). GFP expression is monitored after transfection into HEK293T cells. Following a similar design, the ORF is fused with the protein binding sequences shown in left column of Table 3 below, combined with a cognate sequence inserted in the 3′UTR region of the ORF2 shown in the corresponding right column in the same row. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Exemplary list of elements to enhance translation 
               
               
                 efficiency and stability of the LINE-1 proteins 
               
               
                 and increased expression of LINE-1 proteins. 
               
            
           
           
               
               
               
            
               
                   
                 Elements to be fused 
                 3′ UTR sequence 
               
               
                   
                 with the LINE-1 ORF2 
                 recognizable by the element 
               
               
                   
                   
               
               
                   
                 PP7 coat protein 
                 PP7 
               
               
                   
                 Streptavidin 
                 S1m aptamer 
               
               
                   
                 Tobramycin 
                 Tobramycin aptamer 
               
               
                   
                   
               
            
           
         
       
     
     V. Modifying the endonuclease function of the retrotransposon. In this example, the constructs are modified to test increase in GFP expression as follows. In a first experimental set, the LINE-1 plasmid GFP is cut at the 3′end of the endonuclease coding sequence of ORF2, and a sequence encoding the DNA binding domain (DBD) of a heterologous zinc finger protein (ZFP) is inserted. In another experimental set, the endonuclease domain is fused with a CRISPR nuclease. A variety of nucleases can be tested by modifying the LINE-1 plasmid GFP ORF by creating a fusion protein using DNA binding domains and cleavage domain as shown in a non-exhaustive list in Table 4, In addition, two ORF-2 domains are encoded in one set to facilitate dimerization. The construct that has higher GFP expression than the ORF2 endonuclease can be further selected. The plasmid designs are graphically represented in  FIG.  25   . GFP expression is monitored after transfection of the plasmids into HEK293T cells, and the set that yielded best. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Exemplary non-exhaustive list of additional DNA 
               
               
                 cleavage domains/enzymes that can be fused to 
               
               
                 or inserted in place of LINE-1 endonuclease. 
               
            
           
           
               
               
               
            
               
                   
                 Gene/Enzyme 
                 Description 
               
               
                   
                   
               
               
                   
                 Fokl 
                 Class II endonuclease from Flavobacterium 
               
               
                   
                   
                 okeanokoites, recognition and cleavage 
               
               
                   
                   
                 sequence are separated by a few 
               
               
                   
                   
                 nucleotides; recognizes DNA sequence 
               
               
                   
                   
                 5-GGATG-3′ 
               
               
                   
                 Restriction 
                 e.g., HindII, EcoR1, BamH1 
               
               
                   
                 enzymes, 
               
               
                   
                 LAGLIDADG 
                 Intron encoded homing proteins found 
               
               
                   
                 family nuclease A 
                 in various genera including bacteria 
               
               
                   
                 (“LAGLIDADG” 
               
               
                   
                 disclosed as 
               
               
                   
                 SEQ ID NO: 66) 
               
               
                   
                 GIY-YIG 
                 This domain is found in the amino 
               
               
                   
                   
                 terminal region of excinuclease abc 
               
               
                   
                   
                 subunit c (uvrC), bacteriophage T4, 
               
               
                   
                   
                 endonuclease segA, segB, seg C, seg D, 
               
               
                   
                   
                 and seg E and group I introns of fungi 
               
               
                   
                   
                 and phage. 
               
               
                   
                 His-Cys box 
                 Homing endonucleases containing two 
               
               
                   
                   
                 clusters of conserved histidine and 
               
               
                   
                   
                 cysteine residues over a 100 amino 
               
               
                   
                   
                 acid region. 
               
               
                   
                 H—N—H 
                 Widely present nuclease in phage DNA. 
               
               
                   
                   
                 Crucial component of the terminase 
               
               
                   
                   
                 packaging reaction of  E. coli  phage HK97. 
               
               
                   
                 PD-(D/E)xK 
                 Phosphodiesterases, present in a large 
               
               
                   
                   
                 number of proteins, e.g., DUF4420, 
               
               
                   
                   
                 DUF3883, DUF4263, COG5482, COG1395, 
               
               
                   
                   
                 Tsp45I, HaeII, Eco47II, ScaI, HpaII. 
               
               
                   
                 Vsr-like/EDxHD 
                 C-terminal nuclease domain that 
               
               
                   
                   
                 displays recognizable homology to 
               
               
                   
                   
                 bacterial Very short repair (Vsr) 
               
               
                   
                   
                 endonucleases 
               
               
                   
                   
               
            
           
         
       
     
     VI. Modifying the reverse transcriptase function of the retrotransposon. In this example, the reverse transcriptase domain of ORF2 is modified for increasing its efficiency. In one experimental set, the sequence encoding the human ORF2 in LINE-1plasmid GFP is excised and replaced with a sequence encoding MMLV or TGIRTII In another experimental set, the ORF2 reverse transcriptase domain is fused with a DNA binding domain of a heterologous protein. The reverse transcriptase domains and/or the DNA binding domains can be selected from a non-exhaustive list provided in Table 5A-Table 5B. The constructs are graphically exemplified in  FIG.  26   . GFP expression is monitored after transfection into HEK293T cells. 
     
       
         
           
               
             
               
                 TABLE 5A 
               
             
            
               
                   
               
               
                 Selected non-exhaustive list of reverse transcriptase 
               
               
                 for replacing the LINE-1 RT for higher efficiency 
               
            
           
           
               
               
               
            
               
                   
                 Reverse Transcriptase 
                 Description 
               
               
                   
                   
               
               
                   
                 M-MLV-RT 
                 Murine leukemia virus 
               
               
                   
                 TGIRT-II 
                 Thermostable group II intron 
               
               
                   
                   
                 reverse transcriptase with 
               
               
                   
                   
                 high fidelity and processivity 
               
               
                   
                 AMV-RT 
                 Avian Myeloblastosis Virus 
               
               
                   
                   
                 reverse transcriptase 
               
               
                   
                 Group II intron 
                 Derived from  Eubacterium rectale   
               
               
                   
                 maturase RT 
               
               
                   
                 HIV-RT 
                 Efficient RT derived from HIV 
               
               
                   
                 TERT 
                 Catalyzes the RNA-dependent 
               
               
                   
                   
                 extension of 3′-chromosomal 
               
               
                   
                   
                 termini with the 6-nucleotide telomeric 
               
               
                   
                   
                 repeat unit, 5′-TTAGGG-3′. 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5B 
               
               
                   
               
               
                 Selected non-exhaustive list of DNA-binding domains 
               
               
                 for fusing to a RT for higher efficiency 
               
               
                 DNA binding domains (DBD) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                   
                 Zinc finger domains 
               
               
                   
                 Leucine zipper (bZip) 
               
               
                   
                 Helix-turn-helix domain 
               
               
                   
                 HMG-box 
               
               
                   
                 R2 retroelement DBD 
               
               
                   
                 Sso7d 
               
               
                   
                 Protein A (ssDNA) 
               
               
                   
                 OB-fold (ssDNA) 
               
               
                   
                   
               
            
           
         
       
     
     VII. Replacing human LINE-1 with LINE-1 from other organisms. In this example, the sequence encoding human LINE-1 is replaced by a LINE-1 from a different organism. In one example, the human LINE-1 construct is compared with a construct where the human LINE-1 is replaced by a minke whale LINE-1 sequence ( FIG.  27   ). Using the same experimental framework, a number of ORFs are tested. An exemplary non-exhaustive list is provided in Table 6 below. A further comprehensive list is available in Ivancevic A. et al., Genome Biol Evol 8(11):3301-3322. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Exemplary LINE-1 elements from organism for 
               
               
                 use in replacement of the human LINE-1 
               
            
           
           
               
               
            
               
                   
                 No of total LINE-1/ 
               
               
                 Species Name 
                 No active/percent active 
               
               
                   
               
               
                 
                   Balaenoptera acutorostrata scammoni 
                 
                 8,012/5,006/62.4% 
               
               
                 
                   Rhinopithecus roxellana 
                 
                 11,115/2,954/26.5% 
               
               
                 
                   Mus musculus 
                 
                 18,280/4,143/22.66% 
               
               
                 
                   Aedes aegypti 
                 
                 519/184/35.4% 
               
               
                 
                   Zea mays 
                 
                 744/165/22.17% 
               
               
                 
                   Brassica napus 
                 
                 1,929/565/29.2% 
               
               
                 
                   Brassica rapa 
                 
                 543/228/41.9% 
               
               
                 
                   Danio rerio 
                 
                 590/268/45.4% 
               
               
                   
               
            
           
         
       
     
     In another set, human LINE-1 is retained as in the GFP plasmid, but an inhibitor of human LINE-1 silencer is utilized to prevent recognition by endogenous proteins like HUSH complex TASOR protein. 
     In this case, the TASOR inhibitor is an inhibitory RNA, such as a miRNA. 
     VIII. LINE-1 fusion proteins for target specificity. In this example, the LINE-1 plasmid GFP ORF2 is fused with a domain of a MegaTAL nuclease, a CRISPR-CAS nuclease, a TALEN, R2 retroelement binding zinc finger binding domain, or a DNA binding domain that can bind to repetitive elements such as Rep78 AAV.  FIG.  28    exemplifies the deigns. Table 7 provides a list of the different elements that can be fused to increase sequence specific retrotransposition. 
     
       
         
           
               
             
               
                 TABLE 7 
               
               
                   
               
               
                 Exemplary proteins with DNA binding domains to be fused to 
               
               
                 ORF2 for increasing retrotransposition specificity 
               
               
                 Elements 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                   
                 Transcription Factors 
               
               
                   
                 MegaTAL nucleases 
               
               
                   
                 TALENs 
               
               
                   
                 Zinc finger binding domains 
               
               
                   
                 from other retroelements 
               
               
                   
                 Safe harbor binding proteins 
               
               
                   
                 Cfp1 
               
               
                   
                   
               
            
           
         
       
     
     Each plasmid is transfected into HEK293 cells and GFP expression is monitored. 
     The modifications described in this section under (I)-(VIII) are designed to test for increase in retrotransposition efficiency, using GFP as readout. Following this, a number of useful modifications from (I)-(VIII) are incorporated into a single retrotransposition construct, tested with GFP as insert for the outcome, and the GFP sequence is replaced by the desired insert sequence. 
     Example 15. Delivering a Large Payload for Prolonged Expression Using Retrotransposon Technology 
     Provided here are exemplary demonstrations of retrotransposon constructs are versatile for incorporating nucleic acid payloads into the genome of a cell and expressing an exemplary transgene. Retrotransposon constructs were designed as elaborated elsewhere in the disclosure. 
     Briefly, in one set of validation experiments, GFP encoding payloads were constructed as follows: an antisense promoter sequence under doxycycline inducible control followed by antisense GFP gene split with an intron in the sense direction was placed downstream of the LINE-1 ORFs ( FIG.  29   ). Splicing donor (SD) and splicing acceptor (SA) sequences are recognized and spliced out only when the mRNA is produced from the promoter in the top strand, therefore only the GFP gene integrated into genome from spliced mRNA generates fluorescent signal. As shown in the representative flow cytometry data in  FIG.  2   , the GFP expression was measured 35 days post doxycycline induction of the ORF expression using flow cytometry (green histogram) compared to a negative control plasmid (grey histogram). In this case, the cargo size was 2.4 kb. 
     The cargo GFP gene in the previous construct was replaced with intron interrupted CD5-FcR-P13K CAR-M sequence (Morrissey et al., 2018). The CD5 binder expression was measured by flow cytometry using a Alexa647-conjugated CD5 protein such that retrotransposed cells are CD5-AF647 positive (red histogram) compared with a plasmid transfected negative control cell population (grey histogram) ( FIG.  30   ). Successful expression of the 3.0 kb construct was demonstrated as shown in the figure. 
     The cargo gene length was extended by adding the intron-interrupted GFP gene after the T2A sequence downstream of the CD5-FcR-PI3K CAR-M sequence ( FIG.  31   ). The CD5 binder expression was measured by flow cytometry using a Alexa647-conjugated CD5 protein. The CD5 binder positive cells shown by red histogram, in comparison with a negative control (grey histogram). The GFP expression is measured using flow cytometry (green histogram) compared to a negative control plasmid transfected cells (grey histogram). The flow cytometry signal in the Q2 showed that 10.8% cells express both CAR-M and GFP proteins. 
     As shown in  FIG.  32   , the payload size limit has not been reached with retrotransposon delivery and integration (Retro-T delivery) with a 3.9 kb payload. The delivery mechanism described here was successful for expression of the first generation CART construct and GFP (separated by T2A site). In this example, different constructs were tested for retrotransposition efficiency of the insert sequence.  FIG.  33 A  shows gene delivery as mRNA results in successful integration. This data is the first to show that Retro T can be delivered as mRNA. A trans strategy of using separate mRNAs encoding for ORF1 and ORF2 with antisense promoter and GFP cargo (ORF2-GFPai) in the 3′ UTR for gene delivery was explored, as exemplified graphically in  FIG.  33 B  (top panel).  FIGS.  33 B- 33 D  demonstrate experimental results from multiple representative assays. Separate mRNAs that expression the LINE-1 proteins could reconstitute the RNA-protein complex required for retrotransposition. The cis strategy uses a single bicistronic LINE-1 mRNA with the antisense promoter and GFP gene cargo in the 3′UTR. Constructs comprising variable amounts and proportions of ORF2 and ORF1 were compared as shown in  FIG.  33 B  and  FIG.  33 C  with GFP encoding sequence as payload.  FIG.  33 D  shows that introducing a single mRNA yields higher number of integrations per cell. Sorting of 293T GFP cells to enrich for retrotransposed cells for biochemical and integration assays. Cells are the same as in  FIG.  33 B  and show GFP expression 4 days post-sort in bottom panels. The graph shows qPCR assay for genomic DNA integration from different LINE-1 plasmid transfected, LINE-1 mRNA (retro-mRNA), and ORF1 and ORF2-GFP mRNA electroporated cells. Two qPCR primer-probe sets were used, one for the housekeeping gene RPS30 and the other for the GFP gene. Plasmid-transfected cells use a plasmid that does not contain and SV40 maintenance sequence. Integration per cell is calculated from determining copy numbers per samples through interpolation of a standard curve of plasmid and genomic DNA and normalizing for the two copies of RPS30 per 293T cell. Error bars denote standard deviation of three technical replicate measurements. 
     Example 16. Delivery to Diverse Cell Types 
     As shown in  FIGS.  34 - 38   , the mRNA constructs comprising a gene of interest, e.g. encoding a CAR protein, or for example, a GFP protein can be efficiently expressed in diverse cell types, such as epithelial cells (e.g., HEK 293 cells), monocytic cells lines (e.g., THP-1 cells), lymphoblastic cell lines (e.g., K562 cells), and primary lymphocytes (T cells). Activated primary T cells were also successfully transfected with mRNA with genomic integration and expression of GFP ( FIG.  36   ). Primary T cells were isolated and expanded using IL7/IL15; and a 1 Gen CAR construct was delivered on day 2 post activation. Cells sorted and frozen. GFP expression was detectable after a freeze-thaw cycle ( FIG.  37 A-B ). This indicates the versatile nature of mRNA mediated delivery and L1-transposon mediated integration.  FIG.  38    shows a representative assay of GFP mRNA integration and expression in 293T cells, K562 cells, THP-1 cells and Primary T cells. 
     Example 17. Modifying the Retrotransposition System, Exchanging for Site-Specificity of an Integrase for Genomic Integration 
     In this prophetic example, large mRNA constructs are stably incorporated and expressed in non-dividing mammalian cell using the retrotransposon integration system into the genome of the cell with a high degree of location specificity using serine integrases. Retrotransposon constructs are designed as elaborated elsewhere in the disclosure. 
     In this example a first construct, an mRNA construct is designed to include Cas endonuclease fused with a serine integrase comprising a mutation in the catalytic domain that eliminates a possibility of double stranded polynucleotide integration by the integrase, and prime editing guide RNA (pegRNA) oligonucleotides directed to a specific genomic locus, e.g., a histone gene locus, an actin gene locus etc, depending on which the guide RNA is specifically designed. One or more T2A cleavage sites separate the coding sequence of the individual proteins, e.g. Cas9, and the serine integrase of the fragment thereof. The pegRNAs are designed to insert a 41 bp AttB landing site. In one or more alternative designs, a LINE 1 ORF2 binding site is incorporated that is located conveniently and in proximity (e.g., juxtaposed) for binding of an ORF2 protein in relation to the integrase binding site. 
     A second mRNA construct is designed to include a LINE1 mRNA fused with a cargo sequence that is greater than 5, 6, 7, 8, 9, 10 kilobases, and encoding a protein; a mutated endonuclease, and an AttP attachment site fused to the cargo sequence for the integrase to attract and bring the cargo sequence to the landing sequence. The AttP attachment site is complementary to the AttB landing site. 
     The mRNA constructs are prepared through in vitro transcription and then purified. Each of resulting purified mRNA constructs are incorporated separately into lipid nanoparticles (LNP). Using electroporation, the two mRNA constructs are co-delivered using LNPs into cell. The Cas9 endonuclease fused to the pegRNAs is guided to insert the genomic landing sequence into the specific gene locus. The AttB landing site acts like a beacon that attracts for the AttP attachment site, which by design is comprised within the cargo sequence that is associated with the LINE1 mRNA. Co-translation of the proteins lead to generation of the ORF polypeptides, and led by the integrase and tethered by the attachment site, the ORF contacts with the associated ORF binding site at the proximity, and integrates the cargo at the specific site. 
     In yet another alternative design system, the integrase system is altogether bypassed and the PEG-RNA incorporates only a ORF binding site at a specific genomic locus directed by the guide RNA. 
     Site-specific integration is confirmed by Sanger sequencing. Flow cytometry is performed to demonstrate the expression of the insert sequence in the cell. Cell survival and genomic integration is found to be higher than often found in plasmid- or vector-based systems, as the mRNA has less of a detrimental impact on the cells. 
     Example 18. Exemplary Sequences 
     Following are exemplary sequences of the constructs used in the examples. These sequences are for reference exemplary purposes and sequence variations and optimizations that are conceivable by one of skill in the art without undue experimentation are contemplated and encompassed by the disclosure. Where mRNA sequences are referred in the sequence title, the construct recites nucleotides of a DNA template and one of skill in the art can easily derive the corresponding mRNA sequence. 
     
       
         
           
               
             
               
                 TABLE 8 
               
               
                   
               
               
                 Plasmid and mRNA construct sequences 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                   
                 ORF1-FLAG-mRNA (Codon Optimized human 
               
               
                   
                 ORF1 coding sequence-FLAG) (SEQ ID NO: 35): 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAAGACGCCACCATGGGCAAGAA 
               
               
                   
                 GCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCCC 
               
               
                   
                 ACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTG 
               
               
                   
                 GATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCG 
               
               
                   
                 ATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGAA 
               
               
                   
                 GGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCG 
               
               
                   
                 TATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAA 
               
               
                   
                 GACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGATC 
               
               
                   
                 CAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGA 
               
               
                   
                 GATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCAT 
               
               
                   
                 TAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCAA 
               
               
                   
                 GAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGA 
               
               
                   
                 AAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAGA 
               
               
                   
                 AAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAGA 
               
               
                   
                 GATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCC 
               
               
                   
                 TAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAA 
               
               
                   
                 GATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGGG 
               
               
                   
                 CAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCA 
               
               
                   
                 GGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAA 
               
               
                   
                 GAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTAT 
               
               
                   
                 CTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCG 
               
               
                   
                 AGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGA 
               
               
                   
                 GGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGAA 
               
               
                   
                 CCACGCAAAGATGGATTATAAGGATGACGATGATAAATGA 
               
               
                   
                 (SEQ ID NO: 35) 
               
               
                   
                   
               
               
                   
                 ORF2-FLAG-GFP aim RNA (Codon Optimized 
               
               
                   
                 human ORF2 coding sequence) (SEQ ID NO: 36) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAAGACGCCACCATGACAGGTTC 
               
               
                   
                 AAATAGTCACATTACGATTCTCACTCTGAATATAAATGGGCTGAA 
               
               
                   
                 TTCTGCAATTAAACGGCACAGGCTTGCTTCCTGGATAAAGTCTCA 
               
               
                   
                 AGACCCCTCAGTGTGCTGTATTCAGGAAACGCATCTCACGTGCAG 
               
               
                   
                 GGACACCCATCGGCTGAAAATAAAAGGCTGGCGGAAGATCTACCA 
               
               
                   
                 AGCCAATGGAAAACAAAAGAAGGCTGGGGTGGCGATACTTGTAAG 
               
               
                   
                 CGATAAAACAGACTTTAAACCAACTAAGATCAAACGGGACAAAGA 
               
               
                   
                 GGGCCATTACATCATGGTAAAGGGTAGTATTCAACAAGAGGAGCT 
               
               
                   
                 GACTATCCTGAATATTTATGCACCTAATACTGGAGCCCCCAGATT 
               
               
                   
                 CATAAAGCAAGTGTTGAGTGACCTTCAACGCGACCTCGACTCCCA 
               
               
                   
                 CACTCTGATCATGGGAGACTTTAACACCCCGCTGTCCACTCTCGA 
               
               
                   
                 CAGATCTACTAGACAGAAAGTCAACAAGGATACACAGGAACTGAA 
               
               
                   
                 CAGTGCTCTCCACCAAGCGGACCTTATCGACATCTACAGAACACT 
               
               
                   
                 CCACCCCAAAAGCACAGAATATACCTTCTTTTCAGCCCCTCACCA 
               
               
                   
                 CACCTATTCCAAAATTGACCACATTGTGGGGAGTAAAGCCCTTCT 
               
               
                   
                 CTCCAAATGTAAACGGACCGAAATTATCACTAACTATCTCTCCGA 
               
               
                   
                 CCACAGTGCAATAAAACTTGAATTGCGAATTAAGAATCTCACTCA 
               
               
                   
                 AAGTAGATCCACGACATGGAAACTGAACAATCTCCTCTTGAATGA 
               
               
                   
                 CTACTGGGTGCATAACGAAATGAAGGCTGAAATAAAGATGTTCTT 
               
               
                   
                 TGAGACCAACGAAAACAAAGACACCACGTACCAGAATCTCTGGGA 
               
               
                   
                 CGCTTTCAAAGCAGTGTGTCGAGGAAAATTTATTGCACTGAATGC 
               
               
                   
                 TTACAAGCGGAAGCAGGAAAGATCCAAAATAGACACCCTGACTAG 
               
               
                   
                 CCAACTTAAAGAACTGGAAAAGCAAGAGCAAACTCATAGCAAAGC 
               
               
                   
                 TAGCCGTCGCCAAGAAATTACGAAAATCAGAGCTGAACTGAAGGA 
               
               
                   
                 AATTGAGACACAGAAAACCCTGCAAAAGATAAATGAAAGCCGCAG 
               
               
                   
                 CTGGTTCTTTGAACGCATCAACAAAATCGATAGGCCACTTGCTCG 
               
               
                   
                 CCTTATCAAGAAGAAAAGGGAGAAGAATCAAATCGACACTATAAA 
               
               
                   
                 GAATGATAAAGGCGATATAACCACCGATCCCACAGAAATTCAAAC 
               
               
                   
                 AACCATACGCGAATACTACAAACACCTCTACGCCAATAAACTCGA 
               
               
                   
                 AAATCTCGAGGAAATGGATACATTCCTCGACACGTACACCCTTCC 
               
               
                   
                 CAGGCTGAACCAGGAAGAAGTTGAATCACTGAATCGGCCTATCAC 
               
               
                   
                 GGGGAGTGAAATAGTAGCTATCATCAATTCACTCCCTACCAAGAA 
               
               
                   
                 GTCACCCGGACCTGATGGATTCACCGCCGAATTCTACCAGAGATA 
               
               
                   
                 CATGGAAGAACTGGTGCCCTTCTTGCTGAAACTTTTCCAAAGTAT 
               
               
                   
                 TGAGAAAGAGGGAATACTTCCAAACTCATTTTATGAGGCATCCAT 
               
               
                   
                 CATTCTGATCCCGAAGCCCGGCAGGGACACGACCAAGAAAGAGAA 
               
               
                   
                 TTTTCGACCAATCTCATTGATGAACATTGATGCAAAGATCCTCAA 
               
               
                   
                 TAAAATACTGGCAAATCGGATTCAGCAGCACATAAAGAAGCTGAT 
               
               
                   
                 CCACCATGATCAAGTAGGCTTCATCCCCGGTATGCAAGGTTGGTT 
               
               
                   
                 CAATATACGAAAATCAATCAATGTTATCCAGCATATAAACCGGGC 
               
               
                   
                 CAAAGACAAGAACCACATGATTATTAGTATCGATGCTGAGAAAGC 
               
               
                   
                 CTTTGACAAAATACAACAACCCTTCATGCTGAAAACATTGAATAA 
               
               
                   
                 GCTGGGAATTGATGGCACCTACTTCAAAATCATCAGAGCCATATA 
               
               
                   
                 TGACAAACCAACAGCAAATATCATTCTGAATGGTCAGAAATTGGA 
               
               
                   
                 AGCATTCCCCTTGAAAACCGGCACACGGCAGGGTTGCCCTCTGTC 
               
               
                   
                 ACCACTCCTCTTCAACATCGTGTTGGAAGTTCTTGCCCGCGCAAT 
               
               
                   
                 CCGGCAGGAAAAGGAAATCAAGGGCATTCAACTGGGCAAAGAGGA 
               
               
                   
                 AGTTAAATTGAGCCTGTTTGCAGACGACATGATCGTCTATTTGGA 
               
               
                   
                 AAACCCCATAGTTAGTGCACAAAATCTGCTGAAGTTGATCAGTAA 
               
               
                   
                 TTTCTCCAAAGTGAGTGGGTACAAAATCAATGTGCAAAAGAGCCA 
               
               
                   
                 AGCTTTCTTGTACACCAACAACAGGCAAACTGAGTCTCAAATCAT 
               
               
                   
                 GGGCGAACTCCCCTTCGTGATTGCATCCAAGCGGATCAAATACCT 
               
               
                   
                 GGGGATTCAATTGACTCGTGATGTGAAGGACCTCTTCAAGGAGAA 
               
               
                   
                 CTACAAACCCCTGCTCAAGGAAATCAAAGAGGACACAAACAAATG 
               
               
                   
                 GAAGAACATTCCATGCTCTTGGGTGGGAAGGATCAATATCGTCAA 
               
               
                   
                 AATGGCCATCCTGCCCAAGGTAATTTACAGGTTCAATGCTATACC 
               
               
                   
                 CATCAAGCTCCCCATGACATTCTTCACAGAACTTGAAAAGACGAC 
               
               
                   
                 GCTGAAGTTCATTTGGAACCAGAAACGTGCCAGGATTGCTAAATC 
               
               
                   
                 TATTCTCTCCCAAAAGAACAAAGCTGGCGGAATCACACTCCCAGA 
               
               
                   
                 CTTCAAACTTTACTACAAGGCGACCGTGACGAAAACGGCTTGGTA 
               
               
                   
                 CTGGTACCAAAACAGGGATATAGATCAATGGAACCGAACGGAGCC 
               
               
                   
                 CAGCGAAATTATGCCTCATATATACAACTATCTGATCTTTGACAA 
               
               
                   
                 ACCGGAGAAGAACAAGCAATGGGGAAAGGATAGTCTGTTTAATAA 
               
               
                   
                 ATGGTGCTGGGAAAACTGGCTCGCAATCTGTAGGAAGCTGAAACT 
               
               
                   
                 GGATCCATTCTTGACGCCTTATACAAAGATAAATTCCCGATGGAT 
               
               
                   
                 TAAAGATCTCAACGTGAAACCCAAAACAATTAAAACCCTCGAGGA 
               
               
                   
                 AAACCTGGGTATTACGATTCAGGACATTGGGGTGGGAAAGGACTT 
               
               
                   
                 CATGTCCAAAACCCCAAAAGCGATGGCAACCAAAGACAAAATCGA 
               
               
                   
                 CAAATGGGATCTCATAAAACTTAAGTCATTTTGCACAGCTAAAGA 
               
               
                   
                 AACGACAATTAGGGTGAACCGACAACCGACCACTTGGGAGAAAAT 
               
               
                   
                 CTTCGCAACATACAGTTCTGACAAAGGCCTGATTTCCAGGATCTA 
               
               
                   
                 CAATGAATTGAAACAAATTTACAAGAAGAAGACGAACAACCCTAT 
               
               
                   
                 AAAGAAATGGGCCAAGGACATGAACAGACACTTCTCTAAGGAAGA 
               
               
                   
                 CATTTATGCAGCCAAGAAACACATGAAGAAATGCAGCTCTTCACT 
               
               
                   
                 GGCAATCAGGGAAATGCAAATCAAAACAACAATGAGATATCATCT 
               
               
                   
                 CACACCCGTCAGAATGGCCATCATTAAGAAGAGCGGAAACAACCG 
               
               
                   
                 GTGCTGGCGTGGTTGCGGAGAAATCGGTACTCTCCTTCACTGTTG 
               
               
                   
                 GTGGGACTGTAAACTCGTTCAACCACTGTGGAAGTCTGTGTGGCG 
               
               
                   
                 GTTCCTCAGAGATCTGGAACTCGAAATCCCATTTGACCCAGCCAT 
               
               
                   
                 CCCTCTCCTGGGTATATACCCGAATGAGTATAAATCCTGCTGCTA 
               
               
                   
                 TAAAGACACCTGCACAAGGATGTTTATTGCAGCTCTCTTCACAAT 
               
               
                   
                 CGCGAAGACGTGGAACCAACCCAAATGTCCGACTATGATTGACTG 
               
               
                   
                 GATTAAGAAGATGTGGCACATATACACTATGGAATACTATGCTGC 
               
               
                   
                 GATCAAGAACGATGAGTTCATATCATTTGTGGGCACATGGATGAA 
               
               
                   
                 ACTCGAAACCATCATACTCTCTAAATTGAGTCAAGAACAGAAAAC 
               
               
                   
                 TAAACACCGTATATTTTCCCTGATCGGTGGGAATTAGCTACAAAG 
               
               
                   
                 ACGATGACGACAAGGACCATGGAGACGGTGAGAGACACAAAAAAT 
               
               
                   
                 TCCAACACACTATTGCAATGAAAATAAATTTCCTTTATTAGCCAG 
               
               
                   
                 AAGTCAGATGCTCAAGGGGCTTCATGATGTCCCCATAATTTTTGG 
               
               
                   
                 CAGAGGGAAAAAGATCTCAGTGGTATTTGTGAGCCAGGGCATTGG 
               
               
                   
                 CCTTCTGATAGGCAGCCTGCACCTGAGGAGTGCGGCCGCTTTACT 
               
               
                   
                 TGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGA 
               
               
                   
                 ACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGC 
               
               
                   
                 TCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCA 
               
               
                   
                 CGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGA 
               
               
                   
                 GCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTCA 
               
               
                   
                 CCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGT 
               
               
                   
                 GGCTGTTGTAGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGT 
               
               
                   
                 CCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGG 
               
               
                   
                 TGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGT 
               
               
                   
                 CGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCA 
               
               
                   
                 TGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGC 
               
               
                   
                 GGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGG 
               
               
                   
                 GCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGG 
               
               
                   
                 TCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCTCGCCGGACACGC 
               
               
                   
                 TGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGG 
               
               
                   
                 GCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                 CGGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTC 
               
               
                   
                 CCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTT 
               
               
                   
                 GTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAA 
               
               
                   
                 ACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGA 
               
               
                   
                 GTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCAT 
               
               
                   
                 CACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGT 
               
               
                   
                 AGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGG 
               
               
                   
                 CCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATG 
               
               
                   
                 ATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTC 
               
               
                   
                 CACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGG 
               
               
                   
                 AACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGG 
               
               
                   
                 TCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGACGTCTCA 
               
               
                   
                 GCTGACAATGAGATCACATGGACACAGGAAGGGGAATATCACACT 
               
               
                   
                 CTGGGGACTGTGGTGGGGTCGGGGGAGGGGGGAGGGATAGCATTG 
               
               
                   
                 GGAGATATACCTAATGCTAGATGACACATTAGTGGGTGCAGCGCA 
               
               
                   
                 CCAGCATGGCACATGTATACATATGTAACTAACCTGCACAATGTG 
               
               
                   
                 CACATGTACCCTAAAACTTAGAGTATAATGGATCCGCAGGCCTCT 
               
               
                   
                 GCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGAC 
               
               
                   
                 ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG 
               
               
                   
                 CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT 
               
               
                   
                 TTATTTGTAACCATTATAAGCTGCAATAAACAAGTT 
               
               
                   
                 (SEQ ID NO: 36) 
               
               
                   
                   
               
               
                   
                 LINE-1 plasmid GFP (SEQ ID NO: 37) 
               
               
                   
                 CGGCCGCGGGGGGAGGAGCCAAGATGGCCGAATAGGAACAGCTCC 
               
               
                   
                 GGTCTACAGCTCCCAGCGTGAGCGACGCAGAAGACGGTGATTTCT 
               
               
                   
                 GCATTTCCATCTGAGGTACCGGGTTCATCTCACTAGGGAGTGCCA 
               
               
                   
                 GACAGTGGGCGCAGGCCAGTGTGTGTGCGCACCGTGCGCGAGCCG 
               
               
                   
                 AAGCAGGGCGAGGCATTGCCTCACCTGGGAAGCGCAAGGGGTCAG 
               
               
                   
                 GGAGTTCCCTTTCCGAGTCAAAGAAAGGGGTGACGGACGCACCTG 
               
               
                   
                 GAAAATCGGGTCACTCCCACCCGAATATTGCGCTTTTCAGACCGG 
               
               
                   
                 CTTAAGAAACGGCGCACCACGAGACTATATCCCACACCTGGCTCG 
               
               
                   
                 GAGGGTCCTACGCCCACGGAATCTCGCTGATTGCTAGCACAGCAG 
               
               
                   
                 TCTGAGATCAAACTGCAAGGCGGCAACGAGGCTGGGGGAGGGGCG 
               
               
                   
                 CCCGCCATTGCCCAGGCTTGCTTAGGTAAACAAAGCAGCAGGGAA 
               
               
                   
                 GCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTG 
               
               
                   
                 CCTCTGTAGGCTCCACCTCTGGGGGCAGGGCACAGACAAACAAAA 
               
               
                   
                 AGACAGCAGTAACCTCTGCAGACTTAAGTGTCCCTGTCTGACAGC 
               
               
                   
                 TTTGAAGAGAGCAGTGGTTCTCCCAGCACGCAGCTGGAGATCTGA 
               
               
                   
                 GAACGGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCTGACCC 
               
               
                   
                 CCGAGCAGCCTAACTGGGAGGCACCCCCCAGCAGGGGCACACTGA 
               
               
                   
                 CACCTCACACGGCAGGGTATTCCAACAGACCTGCAGCTGAGGGTC 
               
               
                   
                 CTGTCTGTTAGAAGGAAAACTAACAACCAGAAAGGACATCTACAC 
               
               
                   
                 CGAAAACCCATCTGTACATCACCATCATCAAAGACCAAAAGTAGA 
               
               
                   
                 TAAAACCACAAAGATGGGGAAAAAACAGAACAGAAAAACTGGAAA 
               
               
                   
                 CTCTAAAACGCAGAGCGCCTCTCCTCCTCCAAAGGAACGCAGTTC 
               
               
                   
                 CTCACCAGCAACAGAACAAAGCTGGATGGAGAATGATTTTGATGA 
               
               
                   
                 GCTGAGAGAAGAAGGCTTCAGACGATCAAATTACTCTGAGCTACG 
               
               
                   
                 GGAGGACATTCAAACCAAAGGCAAAGAAGTTGAAAACTTTGAAAA 
               
               
                   
                 AAATTTAGAAGAATGTATAACTAGAATAACCAATACAGAGAAGTG 
               
               
                   
                 CTTAAAGGAGCTGATGGAGCTGAAAACCAAGGCTCGAGAACTACG 
               
               
                   
                 TGAAGAATGCAGAAGCCTCAGGAGCCGATGCGATCAACTGGAAGA 
               
               
                   
                 AAGGGTATCAGCAATGGAAGATGAAATGAATGAAATGAAGCGAGA 
               
               
                   
                 AGGGAAGTTTAGAGAAAAAAGAATAAAAAGAAATGAGCAAAGCCT 
               
               
                   
                 CCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACGTCTGAT 
               
               
                   
                 TGGTGTACCTGAAAGTGATGTGGAGAATGGAACCAAGTTGGAAAA 
               
               
                   
                 CACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAG 
               
               
                   
                 GCAGGCCAACGTTCAGATTCAGGAAATACAGAGAACGCCACAAAG 
               
               
                   
                 ATACTCCTCGAGAAGAGCAACTCCAAGACACATAATTGTCAGATT 
               
               
                   
                 CACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGA 
               
               
                   
                 GAAAGGTCGGGTTACCCTCAAAGGAAAGCCCATCAGACTAACAGC 
               
               
                   
                 GGATCTCTCGGCAGAAACCCTACAAGCCAGAAGAGAGTGGGGGCC 
               
               
                   
                 AATATTCAACATTCTTAAAGAAAAGAATTTTCAACCCAGAATTTC 
               
               
                   
                 ATATCCAGCCAAACTAAGCTTCATAAGTGAAGGAGAAATAAAATA 
               
               
                   
                 CTTTATAGACAAGCAAATGTTGAGAGATTTTGTCACCACCAGGCC 
               
               
                   
                 TGCCCTAAAAGAGCTCCTGAAGGAAGCGCTAAACATGGAAAGGAA 
               
               
                   
                 CAACCGGTACCAGCCGCTGCAAAATCATGCCAAAATGTAAAGACC 
               
               
                   
                 ATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACC 
               
               
                   
                 AGCTAACATCATAATGACAGGATCAACTTCACACATAACAATATT 
               
               
                   
                 AACTTTAAATATAAATGGACTAAATTCTGCAATTAAAAGACACAG 
               
               
                   
                 ACTGGCAAGTTGGATAAAGAGTCAAGACCCATCAGTGTGCTGTAT 
               
               
                   
                 TCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCAAAAT 
               
               
                   
                 AAAAGGATGGAGGAAGATCTACCAAGCCAATGGAAAACAAAAAAA 
               
               
                   
                 GGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGACTTTAAACC 
               
               
                   
                 AACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGTAAA 
               
               
                   
                 GGGATCAATTCAACAAGAGGAGCTAACTATCCTAAATATTTATGC 
               
               
                   
                 ACCCAATACAGGAGCACCCAGATTCATAAAGCAAGTCCTCAGTGA 
               
               
                   
                 CCTACAAAGAGACTTAGACTCCCACACATTAATAATGGGAGACTT 
               
               
                   
                 TAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGT 
               
               
                   
                 CAACAAGGATACCCAGGAATTGAACTCAGCTCTGCACCAAGCAGA 
               
               
                   
                 CCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATA 
               
               
                   
                 TACATTTTTTTCAGCACCACACCACACCTATTCCAAAATTGACCA 
               
               
                   
                 CATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGA 
               
               
                   
                 AATTATAACAAACTATCTCTCAGACCACAGTGCAATCAAACTAGA 
               
               
                   
                 ACTCAGGATTAAGAATCTCACTCAAAGCCGCTCAACTACATGGAA 
               
               
                   
                 ACTGAACAACCTGCTCCTGAATGACTACTGGGTACATAACGAAAT 
               
               
                   
                 GAAGGCAGAAATAAAGATGTTCTTTGAAACCAACGAGAACAAAGA 
               
               
                   
                 CACCACATACCAGAATCTCTGGGACGCATTCAAAGCAGTGTGTAG 
               
               
                   
                 AGGGAAATTTATAGCACTAAATGCCTACAAGAGAAAGCAGGAAAG 
               
               
                   
                 ATCCAAAATTGACACCCTAACATCACAATTAAAAGAACTAGAAAA 
               
               
                   
                 GCAAGAGCAAACACATTCAAAAGCTAGCAGAAGGCAAGAAATAAC 
               
               
                   
                 TAAAATCAGAGCAGAACTGAAGGAAATAGAGACACAAAAAACCCT 
               
               
                   
                 TCAAAAAATCAATGAATCCAGGAGCTGGTTTTTTGAAAGGATCAA 
               
               
                   
                 CAAAATTGATAGACCGCTAGCAAGACTAATAAAGAAAAAAAGAGA 
               
               
                   
                 GAAGAATCAAATAGACACAATAAAAAATGATAAAGGGGATATCAC 
               
               
                   
                 CACCGATCCCACAGAAATACAAACTACCATCAGAGAATACTACAA 
               
               
                   
                 ACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAC 
               
               
                   
                 ATTCCTCGACACATACACTCTCCCAAGACTAAACCAGGAAGAAGT 
               
               
                   
                 TGAATCTCTGAATCGACCAATAACAGGCTCTGAAATTGTGGCAAT 
               
               
                   
                 AATCAATAGTTTACCAACCAAAAAGAGTCCAGGACCAGATGGATT 
               
               
                   
                 CACAGCCGAATTCTACCAGAGGTACAAGGAGGAACTGGTACCATT 
               
               
                   
                 CCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCC 
               
               
                   
                 TAACTCATTTTATGAGGCCAGCATCATTCTGATACCAAAGCCGGG 
               
               
                   
                 CAGAGACACAACCAAAAAAGAGAATTTTAGACCAATATCCTTGAT 
               
               
                   
                 GAACATTGATGCAAAAATCCTCAATAAAATACTGGCAAACCGAAT 
               
               
                   
                 CCAGCAGCACATCAAAAAGCTTATCCACCATGATCAAGTGGGCTT 
               
               
                   
                 CATCCCTGGGATGCAAGGCTGGTTCAATATACGCAAATCAATAAA 
               
               
                   
                 TGTAATCCAGCATATAAACAGAGCCAAAGACAAAAACCACATGAT 
               
               
                   
                 TATCTCAATAGATGCAGAAAAAGCCTTTGACAAAATTCAACAACC 
               
               
                   
                 CTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACGTA 
               
               
                   
                 TTTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATAT 
               
               
                   
                 CATACTGAATGGGCAAAAACTGGAAGCATTCCCTTTGAAAACCGG 
               
               
                   
                 CACAAGACAGGGATGCCCTCTCTCACCGCTCCTATTCAACATAGT 
               
               
                   
                 GTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAA 
               
               
                   
                 GGGTATTCAATTAGGAAAAGAGGAAGTCAAATTGTCCCTGTTTGC 
               
               
                   
                 AGACGACATGATTGTTTATCTAGAAAACCCCATCGTCTCAGCCCA 
               
               
                   
                 AAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATA 
               
               
                   
                 CAAAATCAATGTACAAAAATCACAAGCATTCTTATACACCAACAA 
               
               
                   
                 CAGACAAACAGAGAGCCAAATCATGGGTGAACTCCCATTCACAAT 
               
               
                   
                 TGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGA 
               
               
                   
                 TGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAAGGA 
               
               
                   
                 AATAAAAGAGGAGACAAACAAATGGAAGAACATTCCATGCTCATG 
               
               
                   
                 GGTAGGAAGAATCAATATCGTGAAAATGGCCATACTGCCCAAGGT 
               
               
                   
                 AATTTACAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTT 
               
               
                   
                 CTTCACAGAATTGGAAAAAACTACTTTAAAGTTCATATGGAACCA 
               
               
                   
                 AAAAAGAGCCCGCATTGCCAAGTCAATCCTAAGCCAAAAGAACAA 
               
               
                   
                 AGCTGGAGGCATCACACTACCTGACTTCAAACTATACTACAAGGC 
               
               
                   
                 TACAGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATAT 
               
               
                   
                 AGATCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATAT 
               
               
                   
                 CTACAACTATCTGATCTTTGACAAACCTGAGAAAAACAAGCAATG 
               
               
                   
                 GGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCT 
               
               
                   
                 AGCCATATGTAGAAAGCTGAAACTGGATCCCTTCCTTACACCTTA 
               
               
                   
                 TACAAAAATCAATTCAAGATGGATTAAAGATTTAAACGTTAAACC 
               
               
                   
                 TAAAACCATAAAAACCCTAGAAGAAAACCTAGGCATTACCATTCA 
               
               
                   
                 GGACATAGGCGTGGGCAAGGACTTCATGTCCAAAACACCAAAAGC 
               
               
                   
                 AATGGCAACAAAAGACAAAATTGACAAATGGGATCTAATTAAACT 
               
               
                   
                 AAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAG 
               
               
                   
                 GCAACCTACAACATGGGAGAAAATTTTTGCAACCTACTCATCTGA 
               
               
                   
                 CAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTA 
               
               
                   
                 CAAGAAAAAAACAAACAACCCCATCAAAAAGTGGGCGAAGGACAT 
               
               
                   
                 GAACAGACACTTCTCAAAAGAAGACATTTATGCAGCCAAAAAACA 
               
               
                   
                 CATGAAGAAATGCTCATCATCACTGGCCATCAGAGAAATGCAAAT 
               
               
                   
                 CAAAACCACTATGAGATATCATCTCACACCAGTTAGAATGGCAAT 
               
               
                   
                 CATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGCGGAGA 
               
               
                   
                 AATAGGAACACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCA 
               
               
                   
                 ACCATTGTGGAAGTCAGTGTGGCGATTCCTCAGGGATCTAGAACT 
               
               
                   
                 AGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCC 
               
               
                   
                 AAATGAGTATAAATCATGCTGCTATAAAGACACATGCACACGTAT 
               
               
                   
                 GTTTATTGCGGCACTATTCACAATAGCAAAGACTTGGAACCAACC 
               
               
                   
                 CAAATGTCCAACAATGATAGACTGGATTAAGAAAATGTGGCACAT 
               
               
                   
                 ATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCAT 
               
               
                   
                 ATCCTTTGTAGGGACATGGATGAAATTGGAAACCATCATTCTCAG 
               
               
                   
                 TAAACTATCGCAAGAACAAAAAACCAAACACCGCATATTCTCACT 
               
               
                   
                 CATAGGTGGGAATTGAACAATGAGATCACATGGACACAGGAAGGG 
               
               
                   
                 GAATATCACACTCTGGGGACTGTGGTGGGGTCGGGGGAGGGGGGA 
               
               
                   
                 GGGATAGCATTGGGAGATATACCTAATGCTAGATGACACATTAGT 
               
               
                   
                 GGGTGCAGCGCACCAGCATGGCACATGTATACGGATCCGAATTCT 
               
               
                   
                 CGACGGATCGATCCGAACAAACGACCCAACACCCGTGCGTTTTAT 
               
               
                   
                 TCTGTCTTTTTATTGCCGATCCCCTCAGAAGAACTCGTCAAGAAG 
               
               
                   
                 GCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTA 
               
               
                   
                 AAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGC 
               
               
                   
                 AATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCGGCCGC 
               
               
                   
                 TTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGG 
               
               
                   
                 TCACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGT 
               
               
                   
                 CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCA 
               
               
                   
                 GCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGT 
               
               
                   
                 CGGCCAGGTGAGTCCAGGAGATGTTTCAGCACTGTTGCCTTTAGT 
               
               
                   
                 CTCGAGGCAACTTAGACAACTGAGTATTGATCTGAGCACAGCAGG 
               
               
                   
                 GTGTGAGCTGTTTGAAGATACTGGGGTTGGGGGTGAAGAAACTGC 
               
               
                   
                 AGAGGACTAACTGGGCTGAGACCCAGTGGCAATGTTTTAGGGCCT 
               
               
                   
                 AAGGAATGCCTCTGAAAATCTAGATGGACAACTTTGACTTTGAGA 
               
               
                   
                 AAAGAGAGGTGGAAATGAGGAAAATGACTTTTCTTTATTAGATTT 
               
               
                   
                 CGGTAGAAAGAACTTTCATCTTTCCCCTATTTTTGTTATTCGTTT 
               
               
                   
                 TAAAACATCTATCTGGAGGCAGGACAAGTATGGTCATTAAAAAGA 
               
               
                   
                 TGCAGGCAGAAGGCATATATTGGCTCAGTCAAAGTGGGGAACTTT 
               
               
                   
                 GGTGGCCAAACATACATTGCTAAGGCTATTCCTATATCAGCTGGA 
               
               
                   
                 CACATATAAAATGCTGCTAATGCTTCATTACAAACTTATATCCTT 
               
               
                   
                 TAATTCCAGATGGGGGCAAAGTATGTCCAGGGGTGAGGAACAATT 
               
               
                   
                 GAAACATTTGGGCTGGAGTAGATTTTGAAAGTCAGCTCTGTGTGT 
               
               
                   
                 GTGTGTGTGTGTGTGTGTGTGAGAGCGTGTGTTTCTTTTAACGTT 
               
               
                   
                 TTCAGCCTACAGCATACAGGGTTCATGGTGGCAAGAAGATAACAA 
               
               
                   
                 GATTTAAATTATGGCCAGTGACTAGTGCTGCAAGAAGAACAACTA 
               
               
                   
                 CCTGCATTTAATGGGAAAGCAAAATCTCAGGCTTTGAGGGAAGTT 
               
               
                   
                 AACATAGGCTTGATTCTGGGTGGAAGCTGGGTGTGTAGTTATCTG 
               
               
                   
                 GAGGCCAGGCTGGAGCTCTCAGCTCACTATGGGTTCATCTTTATT 
               
               
                   
                 GTCTCCTTTCATCTCAACAGCTGCACGCTGCCGTCCTCGATGTTG 
               
               
                   
                 TGGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCG 
               
               
                   
                 GCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTG 
               
               
                   
                 TGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGC 
               
               
                   
                 TCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCG 
               
               
                   
                 CGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCC 
               
               
                   
                 TGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGC 
               
               
                   
                 TTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTC 
               
               
                   
                 AGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTG 
               
               
                   
                 GTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCC 
               
               
                   
                 TCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCG 
               
               
                   
                 TCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCG 
               
               
                   
                 CCCTTGCTCACCATGGTGGCGAATTCGAAGCTTGAGCTCGAGATC 
               
               
                   
                 TGAGTCCGGTAGCGCTAGCGGATCTGACGGTTCACTAAACCAGCT 
               
               
                   
                 CTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGC 
               
               
                   
                 GTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGA 
               
               
                   
                 TTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGA 
               
               
                   
                 CTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATG 
               
               
                   
                 TACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACG 
               
               
                   
                 TAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGG 
               
               
                   
                 GCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGG 
               
               
                   
                 GGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCA 
               
               
                   
                 GTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCT 
               
               
                   
                 ATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGG 
               
               
                   
                 CGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGT 
               
               
                   
                 TATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCC 
               
               
                   
                 GTAATTGATTACTATTAGCCCGGGGGATCCAGACATGATAAGATA 
               
               
                   
                 CATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAA 
               
               
                   
                 ATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAAC 
               
               
                   
                 CATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCA 
               
               
                   
                 TTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG 
               
               
                   
                 CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCGGC 
               
               
                   
                 TGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATG 
               
               
                   
                 CAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGG 
               
               
                   
                 AGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGT 
               
               
                   
                 CGGGGCGCAGCCATGAGGTCGATCGACTCTAGAGGATCGATCCCC 
               
               
                   
                 GCCCCGGACGAACTAAACCTGACTACGACATCTCTGCCCCTTCTT 
               
               
                   
                 CGCGGGGCAGTGCATGTAATCCCTTCAGTTGGTTGGTACAACTTG 
               
               
                   
                 CCAACTGGGCCCTGTTCCACATGTGACACGGGGGGGGACCAAACA 
               
               
                   
                 CAAAGGGAGGCCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAG 
               
               
                   
                 ATCGCCGGGGTTCTCTGACTGTAGTTGACATCCTTATAAATGGAT 
               
               
                   
                 GTGCACATTTGCCAACACTGAGTGGCTTTCATCCTGGAGCAGACT 
               
               
                   
                 TTGCAGTCTGTGGACTGCAACACAACATTGCCTTTATGTGTAACT 
               
               
                   
                 CTTGGCTGAAGCTCTTACACCAATGCTGGGGGACATGTACCTCCC 
               
               
                   
                 AGGGGCCCAGGAAGACTACGGGAGGCTACACCAACGTCAATCAGA 
               
               
                   
                 GGGGCCTGTGTAGCTACCGATAAGCGGACCCTCAAGAGGGCATTA 
               
               
                   
                 GCAATAGTGTTTATAAGGCCCCCTTGTTAACCCTAAACGGGTAGC 
               
               
                   
                 ATATGCTTCCCGGGTAGTAGTATATACTATCCAGACTAACCCTAA 
               
               
                   
                 TTCAATAGCATATGTTACCCAACGGGAAGCATATGCTATCGAATT 
               
               
                   
                 AGGGTTAGTAAAAGGGTCCTAAGGAACAGCGATATCTCCCACCCC 
               
               
                   
                 ATGAGCTGTCACGGTTTTATTTACATGGGGTCAGGATTCCACGAG 
               
               
                   
                 GGTAGTGAACCATTTTAGTCACAAGGGCAGTGGCTGAAGATCAAG 
               
               
                   
                 GAGCGGGCAGTGAACTCTCCTGAATCTTCGCCTGCTTCTTCATTC 
               
               
                   
                 TCCTTCGTTTAGCTAATAGAATAACTGCTGAGTTGTGAACAGTAA 
               
               
                   
                 GGTGTATGTGAGGTGCTCGAAAACAAGGTTTCAGGTGACGCCCCC 
               
               
                   
                 AGAATAAAATTTGGACGGGGGGTTCAGTGGTGGCATTGTGCTATG 
               
               
                   
                 ACACCAATATAACCCTCACAAACCCCTTGGGCAATAAATACTAGT 
               
               
                   
                 GTAGGAATGAAACATTCTGAATATCTTTAACAATAGAAATCCATG 
               
               
                   
                 GGGTGGGGACAAGCCGTAAAGACTGGATGTCCATCTCACACGAAT 
               
               
                   
                 TTATGGCTATGGGCAACACATAATCCTAGTGCAATATGATACTGG 
               
               
                   
                 GGTTATTAAGATGTGTCCCAGGCAGGGACCAAGACAGGTGAACCA 
               
               
                   
                 TGTTGTTACACTCTATTTGTAACAAGGGGAAAGAGAGTGGACGCC 
               
               
                   
                 GACAGCAGCGGACTCCACTGGTTGTCTCTAACACCCCCGAAAATT 
               
               
                   
                 AAACGGGGCTCCACGCCAATGGGGCCCATAAACAAAGACAAGTGG 
               
               
                   
                 CCACTCTTTTTTTTGAAATTGTGGAGTGGGGGCACGCGTCAGCCC 
               
               
                   
                 CCACACGCCGCCCTGCGGTTTTGGACTGTAAAATAAGGGTGTAAT 
               
               
                   
                 AACTTGGCTGATTGTAACCCCGCTAACCACTGCGGTCAAACCACT 
               
               
                   
                 TGCCCACAAAACCACTAATGGCACCCCGGGGAATACCTGCATAAG 
               
               
                   
                 TAGGTGGGCGGGCCAAGATAGGGGCGCGATTGCTGCGATCTGGAG 
               
               
                   
                 GACAAATTACACACACTTGCGCCTGAGCGCCAAGCACAGGGTTGT 
               
               
                   
                 TGGTCCTCATATTCACGAGGTCGCTGAGAGCACGGTGGGCTAATG 
               
               
                   
                 TTGCCATGGGTAGCATATACTACCCAAATATCTGGATAGCATATG 
               
               
                   
                 CTATCCTAATCTATATCTGGGTAGCATAGGCTATCCTAATCTATA 
               
               
                   
                 TCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATG 
               
               
                   
                 CTATCCTAATTTATATCTGGGTAGCATAGGCTATCCTAATCTATA 
               
               
                   
                 TCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATG 
               
               
                   
                 CTATCCTAATCTGTATCCGGGTAGCATATGCTATCCTAATAGAGA 
               
               
                   
                 TTAGGGTAGTATATGCTATCCTAATTTATATCTGGGTAGCATATA 
               
               
                   
                 CTACCCAAATATCTGGATAGCATATGCTATCCTAATCTATATCTG 
               
               
                   
                 GGTAGCATATGCTATCCTAATCTATATCTGGGTAGCATAGGCTAT 
               
               
                   
                 CCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTG 
               
               
                   
                 GGTAGTATATGCTATCCTAATTTATATCTGGGTAGCATAGGCTAT 
               
               
                   
                 CCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTG 
               
               
                   
                 GGTAGTATATGCTATCCTAATCTGTATCCGGGTAGCATATGCTAT 
               
               
                   
                 CCTCATGCATATACAGTCAGCATATGATACCCAGTAGTAGAGTGG 
               
               
                   
                 GAGTGCTATCCTTTGCATATGCCGCCACCTCCCAAGGGGGCGTGA 
               
               
                   
                 ATTTTCGCTGCTTGTCCTTTTCCTGCATGCTGGTTGCTCCCATTC 
               
               
                   
                 TTAGGTGAATTTAAGGAGGCCAGGCTAAAGCCGTCGCATGTCTGA 
               
               
                   
                 TTGCTCACCAGGTAAATGTCGCTAATGTTTTCCAACGCGAGAAGG 
               
               
                   
                 TGTTGAGCGCGGAGCTGAGTGACGTGACAACATGGGTATGCCCAA 
               
               
                   
                 TTGCCCCATGTTGGGAGGACGAAAATGGTGACAAGACAGATGGCC 
               
               
                   
                 AGAAATACACCAACAGCACGCATGATGTCTACTGGGGATTTATTC 
               
               
                   
                 TTTAGTGCGGGGGAATACACGGCTTTTAATACGATTGAGGGCGTC 
               
               
                   
                 TCCTAACAAGTTACATCACTCCTGCCCTTCCTCACCCTCATCTCC 
               
               
                   
                 ATCACCTCCTTCATCTCCGTCATCTCCGTCATCACCCTCCGCGGC 
               
               
                   
                 AGCCCCTTCCACCATAGGTGGAAACCAGGGAGGCAAATCTACTCC 
               
               
                   
                 ATCGTCAAAGCTGCACACAGTCACCCTGATATTGCAGGTAGGAGC 
               
               
                   
                 GGGCTTTGTCATAACAAGGTCCTTAATCGCATCCTTCAAAACCTC 
               
               
                   
                 AGCAAATATATGAGTTTGTAAAAAGACCATGAAATAACAGACAAT 
               
               
                   
                 GGACTCCCTTAGCGGGCCAGGTTGTGGGCCGGGTCCAGGGGCCAT 
               
               
                   
                 TCCAAAGGGGAGACGACTCAATGGTGTAAGACGACATTGTGGAAT 
               
               
                   
                 AGCAAGGGCAGTTCCTCGCCTTAGGTTGTAAAGGGAGGTCTTACT 
               
               
                   
                 ACCTCCATATACGAACACACCGGCGACCCAAGTTCCTTCGTCGGT 
               
               
                   
                 AGTCCTTTCTACGTGACTCCTAGCCAGGAGAGCTCTTAAACCTTC 
               
               
                   
                 TGCAATGTTCTCAAATTTCGGGTTGGAACCTCCTTGACCACGATG 
               
               
                   
                 CTTTCCAAACCACCCTCCTTTTTTGCGCCTGCCTCCATCACCCTG 
               
               
                   
                 ACCCCGGGGTCCAGTGCTTGGGCCTTCTCCTGGGTCATCTGCGGG 
               
               
                   
                 GCCCTGCTCTATCGCTCCCGGGGGCACGTCAGGCTCACCATCTGG 
               
               
                   
                 GCCACCTTCTTGGTGGTATTCAAAATAATCGGCTTCCCCTACAGG 
               
               
                   
                 GTGGAAAAATGGCCTTCTACCTGGAGGGGGCCTGCGCGGTGGAGA 
               
               
                   
                 CCCGGATGATGATGACTGACTACTGGGACTCCTGGGCCTCTTTTC 
               
               
                   
                 TCCACGTCCACGACCTCTCCCCCTGGCTCTTTCACGACTTCCCCC 
               
               
                   
                 CCTGGCTCTTTCACGTCCTCTACCCCGGCGGCCTCCACTACCTCC 
               
               
                   
                 TCGACCCCGGCCTCCACTACCTCCTCGACCCCGGCCTCCACTGCC 
               
               
                   
                 TCCTCGACCCCGGCCTCCACCTCCTGCTCCTGCCCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGC 
               
               
                   
                 CCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGC 
               
               
                   
                 CCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCC 
               
               
                   
                 TGCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCC 
               
               
                   
                 TGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCTCCTGC 
               
               
                   
                 CCCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTCCTGC 
               
               
                   
                 TCCTGCCCCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCC 
               
               
                   
                 TCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCC 
               
               
                   
                 TCCTGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCC 
               
               
                   
                 TCCTCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCC 
               
               
                   
                 TCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCC 
               
               
                   
                 TCCTGCCCCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCC 
               
               
                   
                 TCCTCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCCCGCTCCTGC 
               
               
                   
                 TCCTGCTCCTGTTCCACCGTGGGTCCCTTTGCAGCCAATGCAACT 
               
               
                   
                 TGGACGTTTTTGGGGTCTCCGGACACCATCTCTATGTCTTGGCCC 
               
               
                   
                 TGATCCTGAGCCGCCCGGGGCTCCTGGTCTTCCGCCTCCTCGTCC 
               
               
                   
                 TCGTCCTCTTCCCCGTCCTCGTCCATGGTTATCACCCCCTCTTCT 
               
               
                   
                 TTGAGGTCCACTGCCGCCGGAGCCTTCTGGTCCAGATGTGTCTCC 
               
               
                   
                 CTTCTCTCCTAGGCCATTTCCAGGTCCTGTACCTGGCCCCTCGTC 
               
               
                   
                 AGACATGATTCACACTAAAAGAGATCAATAGACATCTTTATTAGA 
               
               
                   
                 CGACGCTCAGTGAATACAGGGAGTGCAGACTCCTGCCCCCTCCAA 
               
               
                   
                 CAGCCCCCCCACCCTCATCCCCTTCATGGTCGCTGTCAGACAGAT 
               
               
                   
                 CCAGGTCTGAAAATTCCCCATCCTCCGAACCATCCTCGTCCTCAT 
               
               
                   
                 CACCAATTACTCGCAGCCCGGAAAACTCCCGCTGAACATCCTCAA 
               
               
                   
                 GATTTGCGTCCTGAGCCTCAAGCCAGGCCTCAAATTCCTCGTCCC 
               
               
                   
                 CCTTTTTGCTGGACGGTAGGGATGGGGATTCTCGGGACCCCTCCT 
               
               
                   
                 CTTCCTCTTCAAGGTCACCAGACAGAGATGCTACTGGGGCAACGG 
               
               
                   
                 AAGAAAAGCTGGGTGCGGCCTGTGAGGATCAGCTTATCGATGATA 
               
               
                   
                 AGCTGTCAAACATGAGAATTCTTGAAGACGAAAGGGCCTCGTGAT 
               
               
                   
                 ACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTA 
               
               
                   
                 GACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTAT 
               
               
                   
                 TTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAG 
               
               
                   
                 ACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAG 
               
               
                   
                 TATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGC 
               
               
                   
                 GGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAA 
               
               
                   
                 AGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACAT 
               
               
                   
                 CGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCC 
               
               
                   
                 CGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATG 
               
               
                   
                 TGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGG 
               
               
                   
                 TCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACC 
               
               
                   
                 AGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATT 
               
               
                   
                 ATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT 
               
               
                   
                 ACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTT 
               
               
                   
                 GCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACC 
               
               
                   
                 GGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGAT 
               
               
                   
                 GCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGA 
               
               
                   
                 ACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGA 
               
               
                   
                 GGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGC 
               
               
                   
                 TGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTC 
               
               
                   
                 TCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCG 
               
               
                   
                 TATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGA 
               
               
                   
                 ACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCA 
               
               
                   
                 TTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGA 
               
               
                   
                 TTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCT 
               
               
                   
                 TTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTT 
               
               
                   
                 CCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTG 
               
               
                   
                 AGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAA 
               
               
                   
                 ACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACC 
               
               
                   
                 AACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACC 
               
               
                   
                 AAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAA 
               
               
                   
                 GAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTT 
               
               
                   
                 ACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTT 
               
               
                   
                 GGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTG 
               
               
                   
                 AACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTA 
               
               
                   
                 CACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCAC 
               
               
                   
                 GCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAG 
               
               
                   
                 GGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGC 
               
               
                   
                 CTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGA 
               
               
                   
                 GCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAA 
               
               
                   
                 AAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTG 
               
               
                   
                 GCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGT 
               
               
                   
                 GGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCG 
               
               
                   
                 CAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGA 
               
               
                   
                 AGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTAT 
               
               
                   
                 TTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGC 
               
               
                   
                 CGCATAGTTAAGCCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGG 
               
               
                   
                 AAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCA 
               
               
                   
                 TCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCC 
               
               
                   
                 AGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAAC 
               
               
                   
                 CATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCC 
               
               
                   
                 CAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTAT 
               
               
                   
                 TTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAA 
               
               
                   
                 GTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCT 
               
               
                   
                 TGCATGCCTGCAGGTCGGCCGCCACGACCGGTGCCGCCACCATCC 
               
               
                   
                 CCTGACCCACGCCCCTGACCCCTCACAAGGAGACGACCTTCCATG 
               
               
                   
                 ACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTC 
               
               
                   
                 CCCCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCC 
               
               
                   
                 GCCACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTC 
               
               
                   
                 ACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATC 
               
               
                   
                 GGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGG 
               
               
                   
                 ACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATC 
               
               
                   
                 GGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAG 
               
               
                   
                 CAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCC 
               
               
                   
                 GCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGC 
               
               
                   
                 AAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCC 
               
               
                   
                 GAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGC 
               
               
                   
                 AACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGAC 
               
               
                   
                 GTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAG 
               
               
                   
                 CCCGGTGCCTGACGCCCGCCCCACGACCCGCAGCGCCCGACCGAA 
               
               
                   
                 AGGAGCGCACGACCCCATGGCTCCGACCGAAGCCGACCCGGGCGG 
               
               
                   
                 CCCCGCCGACCCCGCACCCGCCCCCGAGGCCCACCGACTCTAGAG 
               
               
                   
                 GATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTT 
               
               
                   
                 AAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAA 
               
               
                   
                 TGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTA 
               
               
                   
                 CAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTT 
               
               
                   
                 TTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATC 
               
               
                   
                 TTATCATGTCTGGATCACTCGCCGATAGTGGAAACCGACGCCCCA 
               
               
                   
                 GCACTCGTCCGAGGGCAAAGGAATAGGGGAGATGGGGGAGGCTAA 
               
               
                   
                 CTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGA 
               
               
                   
                 CGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTT 
               
               
                   
                 GTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGAT 
               
               
                   
                 ACCCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCC 
               
               
                   
                 TTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTC 
               
               
                   
                 GCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCCACTGGCCCC 
               
               
                   
                 GTGGGTTAGGGACGGGGTCCCCCATGGGGAATGGTTTATGGTTCG 
               
               
                   
                 TGGGGGTTATTATTTTGGGCGTTGCGTGGGGTCTGGTCCACGACT 
               
               
                   
                 GGACTGAGCAGACAGACCCATGGTTTTTGGATGGCCTGGGCATGG 
               
               
                   
                 ACCGCATGTACTGGCGCGACACGAACACCGGGCGTCTGTGGCTGC 
               
               
                   
                 CAAACACCCCCGACCCCCAAAAACCACCGCGCGGATTTCTGGCGT 
               
               
                   
                 GCCAAGCTAGTCGACCAATTCTCATGTTTGACAGCTTATCATCGC 
               
               
                   
                 AGATCCGGGCAACGTTGTTGCATTGCTGCAGGCGCAGAACTGGTA 
               
               
                   
                 GGTATGGAAGATCTCTAGAAGCTGGGTACCAGCTGCTAGCAAGCT 
               
               
                   
                 TGCTAGCGGCCGGCTCGAGTTTACTCCCTATCAGTGATAGAGAAC 
               
               
                   
                 GTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGATGTCGAG 
               
               
                   
                 TTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCC 
               
               
                   
                 TATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGA 
               
               
                   
                 TAGAGAACGTATGTCGAGTTTATCCCTATCAGTGATAGAGAACGT 
               
               
                   
                 ATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGG 
               
               
                   
                 TAGGCGTGTACGGTG 
               
               
                   
                 (SEQ ID NO: 37) 
               
               
                   
                   
               
               
                   
                 LINE1-GFP mRNA (SEQ ID NO: 38) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGA 
               
               
                   
                 AGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATA 
               
               
                   
                 GTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTT 
               
               
                   
                 AACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCC 
               
               
                   
                 AGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAG 
               
               
                   
                 ACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGA 
               
               
                   
                 TGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGA 
               
               
                   
                 GTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAA 
               
               
                   
                 ATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGC 
               
               
                   
                 ATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAAC 
               
               
                   
                 ACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAG 
               
               
                   
                 CGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACA 
               
               
                   
                 CCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAA 
               
               
                   
                 GACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATT 
               
               
                   
                 GATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTC 
               
               
                   
                 TTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTC 
               
               
                   
                 GGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATT 
               
               
                   
                 ACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGA 
               
               
                   
                 ATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAAT 
               
               
                   
                 AACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCA 
               
               
                   
                 GAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACC 
               
               
                   
                 TATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAG 
               
               
                   
                 TTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAG 
               
               
                   
                 ATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAA 
               
               
                   
                 CAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATT 
               
               
                   
                 CGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAA 
               
               
                   
                 ATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATA 
               
               
                   
                 GACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAAC 
               
               
                   
                 CAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGAC 
               
               
                   
                 CCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTG 
               
               
                   
                 TATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTG 
               
               
                   
                 GATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCC 
               
               
                   
                 CTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAAC 
               
               
                   
                 TCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCT 
               
               
                   
                 GAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTC 
               
               
                   
                 AAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCC 
               
               
                   
                 TTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGAT 
               
               
                   
                 ACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATC 
               
               
                   
                 GACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAA 
               
               
                   
                 CATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCT 
               
               
                   
                 GGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATT 
               
               
                   
                 CAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCT 
               
               
                   
                 ATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATG 
               
               
                   
                 CTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAG 
               
               
                   
                 ATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTT 
               
               
                   
                 AACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGC 
               
               
                   
                 CAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAG 
               
               
                   
                 GTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATA 
               
               
                   
                 CAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGAT 
               
               
                   
                 ATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTT 
               
               
                   
                 CTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATT 
               
               
                   
                 AACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAG 
               
               
                   
                 ACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGC 
               
               
                   
                 AAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAA 
               
               
                   
                 GATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAG 
               
               
                   
                 GAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGC 
               
               
                   
                 AGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATAT 
               
               
                   
                 CGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACG 
               
               
                   
                 GAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGA 
               
               
                   
                 GCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGT 
               
               
                   
                 GGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTA 
               
               
                   
                 ACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAG 
               
               
                   
                 TGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAAT 
               
               
                   
                 TACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAA 
               
               
                   
                 GACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATA 
               
               
                   
                 TGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAA 
               
               
                   
                 ATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACT 
               
               
                   
                 ATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATA 
               
               
                   
                 GGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCC 
               
               
                   
                 ACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGC 
               
               
                   
                 TTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCC 
               
               
                   
                 ACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGG 
               
               
                   
                 TTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAG 
               
               
                   
                 AAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGG 
               
               
                   
                 CATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAG 
               
               
                   
                 AAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACG 
               
               
                   
                 ACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAG 
               
               
                   
                 AAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGC 
               
               
                   
                 ACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTT 
               
               
                   
                 TGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATT 
               
               
                   
                 CCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAA 
               
               
                   
                 TACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATC 
               
               
                   
                 GCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGC 
               
               
                   
                 CCCACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACC 
               
               
                   
                 ATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTC 
               
               
                   
                 GTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTG 
               
               
                   
                 TCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGT 
               
               
                   
                 GGTAACGACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAGA 
               
               
                   
                 AGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGG 
               
               
                   
                 ATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGA 
               
               
                   
                 TTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGA 
               
               
                   
                 TACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATG 
               
               
                   
                 GCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTT 
               
               
                   
                 TATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGC 
               
               
                   
                 ACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACC 
               
               
                   
                 ACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATT 
               
               
                   
                 GCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACA 
               
               
                   
                 GGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGG 
               
               
                   
                 AAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGG 
               
               
                   
                 ATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAAT 
               
               
                   
                 CCAGCGGACCTTCCTTCCCGCTGAGAGACACAAAAAATTCCAACA 
               
               
                   
                 CACTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAG 
               
               
                   
                 ATGCTCAAGGGGCTTCATGATGTCCCCATAATTTTTGGCAGAGGG 
               
               
                   
                 AAAAAGATCTCAGTGGTATTTGTGAGCCAGGGCATTGGCCTTCTG 
               
               
                   
                 ATAGGCAGCCTGCACCTGAGGAGTGCGGCCGCTTTACTTGTACAG 
               
               
                   
                 CTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGGGC 
               
               
                   
                 GGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCC 
               
               
                   
                 GTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCAC 
               
               
                   
                 GCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTCACCTTGAT 
               
               
                   
                 GCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTT 
               
               
                   
                 GTAGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTT 
               
               
                   
                 GAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCC 
               
               
                   
                 CTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTT 
               
               
                   
                 GAAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGA 
               
               
                   
                 CTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA 
               
               
                   
                 GCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGGG 
               
               
                   
                 CACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTT 
               
               
                   
                 GCCGTAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTT 
               
               
                   
                 GTGGCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCAC 
               
               
                   
                 CCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGGGATC 
               
               
                   
                 TGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGT 
               
               
                   
                 ACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGA 
               
               
                   
                 CATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCA 
               
               
                   
                 TTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAAC 
               
               
                   
                 CGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCATG 
               
               
                   
                 GTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAG 
               
               
                   
                 TCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTA 
               
               
                   
                 CCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACT 
               
               
                   
                 TGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCAT 
               
               
                   
                 TGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATAC 
               
               
                   
                 GTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCA 
               
               
                   
                 GGCGGGCCATTTACCGTAAGTTATGTAACGGGCCTGCTGCCGGCT 
               
               
                   
                 CTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGG 
               
               
                   
                 ATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTG 
               
               
                   
                 AGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGA 
               
               
                   
                 TGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTT 
               
               
                   
                 TATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTAT 
               
               
                   
                 AAGCTGCAATAAACAAGTT 
               
               
                   
                 (SEQ ID NO: 38) 
               
               
                   
                   
               
               
                   
                 LINE-1-plasmid CD5-intron-FCR-PI3K 
               
               
                   
                 (SEQ ID NO: 39) 
               
               
                   
                 CGGCCGCGGGGGGAGGAGCCAAGATGGCCGAATAGGAACAGCTCC 
               
               
                   
                 GGTCTACAGCTCCCAGCGTGAGCGACGCAGAAGACGGTGATTTCT 
               
               
                   
                 GCATTTCCATCTGAGGTACCGGGTTCATCTCACTAGGGAGTGCCA 
               
               
                   
                 GACAGTGGGCGCAGGCCAGTGTGTGTGCGCACCGTGCGCGAGCCG 
               
               
                   
                 AAGCAGGGCGAGGCATTGCCTCACCTGGGAAGCGCAAGGGGTCAG 
               
               
                   
                 GGAGTTCCCTTTCCGAGTCAAAGAAAGGGGTGACGGACGCACCTG 
               
               
                   
                 GAAAATCGGGTCACTCCCACCCGAATATTGCGCTTTTCAGACCGG 
               
               
                   
                 CTTAAGAAACGGCGCACCACGAGACTATATCCCACACCTGGCTCG 
               
               
                   
                 GAGGGTCCTACGCCCACGGAATCTCGCTGATTGCTAGCACAGCAG 
               
               
                   
                 TCTGAGATCAAACTGCAAGGCGGCAACGAGGCTGGGGGAGGGGCG 
               
               
                   
                 CCCGCCATTGCCCAGGCTTGCTTAGGTAAACAAAGCAGCAGGGAA 
               
               
                   
                 GCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTG 
               
               
                   
                 CCTCTGTAGGCTCCACCTCTGGGGGCAGGGCACAGACAAACAAAA 
               
               
                   
                 AGACAGCAGTAACCTCTGCAGACTTAAGTGTCCCTGTCTGACAGC 
               
               
                   
                 TTTGAAGAGAGCAGTGGTTCTCCCAGCACGCAGCTGGAGATCTGA 
               
               
                   
                 GAACGGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCTGACCC 
               
               
                   
                 CCGAGCAGCCTAACTGGGAGGCACCCCCCAGCAGGGGCACACTGA 
               
               
                   
                 CACCTCACACGGCAGGGTATTCCAACAGACCTGCAGCTGAGGGTC 
               
               
                   
                 CTGTCTGTTAGAAGGAAAACTAACAACCAGAAAGGACATCTACAC 
               
               
                   
                 CGAAAACCCATCTGTACATCACCATCATCAAAGACCAAAAGTAGA 
               
               
                   
                 TAAAACCACAAAGATGGGGAAAAAACAGAACAGAAAAACTGGAAA 
               
               
                   
                 CTCTAAAACGCAGAGCGCCTCTCCTCCTCCAAAGGAACGCAGTTC 
               
               
                   
                 CTCACCAGCAACAGAACAAAGCTGGATGGAGAATGATTTTGATGA 
               
               
                   
                 GCTGAGAGAAGAAGGCTTCAGACGATCAAATTACTCTGAGCTACG 
               
               
                   
                 GGAGGACATTCAAACCAAAGGCAAAGAAGTTGAAAACTTTGAAAA 
               
               
                   
                 AAATTTAGAAGAATGTATAACTAGAATAACCAATACAGAGAAGTG 
               
               
                   
                 CTTAAAGGAGCTGATGGAGCTGAAAACCAAGGCTCGAGAACTACG 
               
               
                   
                 TGAAGAATGCAGAAGCCTCAGGAGCCGATGCGATCAACTGGAAGA 
               
               
                   
                 AAGGGTATCAGCAATGGAAGATGAAATGAATGAAATGAAGCGAGA 
               
               
                   
                 AGGGAAGTTTAGAGAAAAAAGAATAAAAAGAAATGAGCAAAGCCT 
               
               
                   
                 CCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACGTCTGAT 
               
               
                   
                 TGGTGTACCTGAAAGTGATGTGGAGAATGGAACCAAGTTGGAAAA 
               
               
                   
                 CACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAG 
               
               
                   
                 GCAGGCCAACGTTCAGATTCAGGAAATACAGAGAACGCCACAAAG 
               
               
                   
                 ATACTCCTCGAGAAGAGCAACTCCAAGACACATAATTGTCAGATT 
               
               
                   
                 CACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGA 
               
               
                   
                 GAAAGGTCGGGTTACCCTCAAAGGAAAGCCCATCAGACTAACAGC 
               
               
                   
                 GGATCTCTCGGCAGAAACCCTACAAGCCAGAAGAGAGTGGGGGCC 
               
               
                   
                 AATATTCAACATTCTTAAAGAAAAGAATTTTCAACCCAGAATTTC 
               
               
                   
                 ATATCCAGCCAAACTAAGCTTCATAAGTGAAGGAGAAATAAAATA 
               
               
                   
                 CTTTATAGACAAGCAAATGTTGAGAGATTTTGTCACCACCAGGCC 
               
               
                   
                 TGCCCTAAAAGAGCTCCTGAAGGAAGCGCTAAACATGGAAAGGAA 
               
               
                   
                 CAACCGGTACCAGCCGCTGCAAAATCATGCCAAAATGTAAAGACC 
               
               
                   
                 ATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACC 
               
               
                   
                 AGCTAACATCATAATGACAGGATCAACTTCACACATAACAATATT 
               
               
                   
                 AACTTTAAATATAAATGGACTAAATTCTGCAATTAAAAGACACAG 
               
               
                   
                 ACTGGCAAGTTGGATAAAGAGTCAAGACCCATCAGTGTGCTGTAT 
               
               
                   
                 TCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCAAAAT 
               
               
                   
                 AAAAGGATGGAGGAAGATCTACCAAGCCAATGGAAAACAAAAAAA 
               
               
                   
                 GGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGACTTTAAACC 
               
               
                   
                 AACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGTAAA 
               
               
                   
                 GGGATCAATTCAACAAGAGGAGCTAACTATCCTAAATATTTATGC 
               
               
                   
                 ACCCAATACAGGAGCACCCAGATTCATAAAGCAAGTCCTCAGTGA 
               
               
                   
                 CCTACAAAGAGACTTAGACTCCCACACATTAATAATGGGAGACTT 
               
               
                   
                 TAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGT 
               
               
                   
                 CAACAAGGATACCCAGGAATTGAACTCAGCTCTGCACCAAGCAGA 
               
               
                   
                 CCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATA 
               
               
                   
                 TACATTTTTTTCAGCACCACACCACACCTATTCCAAAATTGACCA 
               
               
                   
                 CATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGA 
               
               
                   
                 AATTATAACAAACTATCTCTCAGACCACAGTGCAATCAAACTAGA 
               
               
                   
                 ACTCAGGATTAAGAATCTCACTCAAAGCCGCTCAACTACATGGAA 
               
               
                   
                 ACTGAACAACCTGCTCCTGAATGACTACTGGGTACATAACGAAAT 
               
               
                   
                 GAAGGCAGAAATAAAGATGTTCTTTGAAACCAACGAGAACAAAGA 
               
               
                   
                 CACCACATACCAGAATCTCTGGGACGCATTCAAAGCAGTGTGTAG 
               
               
                   
                 AGGGAAATTTATAGCACTAAATGCCTACAAGAGAAAGCAGGAAAG 
               
               
                   
                 ATCCAAAATTGACACCCTAACATCACAATTAAAAGAACTAGAAAA 
               
               
                   
                 GCAAGAGCAAACACATTCAAAAGCTAGCAGAAGGCAAGAAATAAC 
               
               
                   
                 TAAAATCAGAGCAGAACTGAAGGAAATAGAGACACAAAAAACCCT 
               
               
                   
                 TCAAAAAATCAATGAATCCAGGAGCTGGTTTTTTGAAAGGATCAA 
               
               
                   
                 CAAAATTGATAGACCGCTAGCAAGACTAATAAAGAAAAAAAGAGA 
               
               
                   
                 GAAGAATCAAATAGACACAATAAAAAATGATAAAGGGGATATCAC 
               
               
                   
                 CACCGATCCCACAGAAATACAAACTACCATCAGAGAATACTACAA 
               
               
                   
                 ACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAC 
               
               
                   
                 ATTCCTCGACACATACACTCTCCCAAGACTAAACCAGGAAGAAGT 
               
               
                   
                 TGAATCTCTGAATCGACCAATAACAGGCTCTGAAATTGTGGCAAT 
               
               
                   
                 AATCAATAGTTTACCAACCAAAAAGAGTCCAGGACCAGATGGATT 
               
               
                   
                 CACAGCCGAATTCTACCAGAGGTACAAGGAGGAACTGGTACCATT 
               
               
                   
                 CCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCC 
               
               
                   
                 TAACTCATTTTATGAGGCCAGCATCATTCTGATACCAAAGCCGGG 
               
               
                   
                 CAGAGACACAACCAAAAAAGAGAATTTTAGACCAATATCCTTGAT 
               
               
                   
                 GAACATTGATGCAAAAATCCTCAATAAAATACTGGCAAACCGAAT 
               
               
                   
                 CCAGCAGCACATCAAAAAGCTTATCCACCATGATCAAGTGGGCTT 
               
               
                   
                 CATCCCTGGGATGCAAGGCTGGTTCAATATACGCAAATCAATAAA 
               
               
                   
                 TGTAATCCAGCATATAAACAGAGCCAAAGACAAAAACCACATGAT 
               
               
                   
                 TATCTCAATAGATGCAGAAAAAGCCTTTGACAAAATTCAACAACC 
               
               
                   
                 CTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACGTA 
               
               
                   
                 TTTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATAT 
               
               
                   
                 CATACTGAATGGGCAAAAACTGGAAGCATTCCCTTTGAAAACCGG 
               
               
                   
                 CACAAGACAGGGATGCCCTCTCTCACCGCTCCTATTCAACATAGT 
               
               
                   
                 GTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAA 
               
               
                   
                 GGGTATTCAATTAGGAAAAGAGGAAGTCAAATTGTCCCTGTTTGC 
               
               
                   
                 AGACGACATGATTGTTTATCTAGAAAACCCCATCGTCTCAGCCCA 
               
               
                   
                 AAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATA 
               
               
                   
                 CAAAATCAATGTACAAAAATCACAAGCATTCTTATACACCAACAA 
               
               
                   
                 CAGACAAACAGAGAGCCAAATCATGGGTGAACTCCCATTCACAAT 
               
               
                   
                 TGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGA 
               
               
                   
                 TGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAAGGA 
               
               
                   
                 AATAAAAGAGGAGACAAACAAATGGAAGAACATTCCATGCTCATG 
               
               
                   
                 GGTAGGAAGAATCAATATCGTGAAAATGGCCATACTGCCCAAGGT 
               
               
                   
                 AATTTACAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTT 
               
               
                   
                 CTTCACAGAATTGGAAAAAACTACTTTAAAGTTCATATGGAACCA 
               
               
                   
                 AAAAAGAGCCCGCATTGCCAAGTCAATCCTAAGCCAAAAGAACAA 
               
               
                   
                 AGCTGGAGGCATCACACTACCTGACTTCAAACTATACTACAAGGC 
               
               
                   
                 TACAGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATAT 
               
               
                   
                 AGATCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATAT 
               
               
                   
                 CTACAACTATCTGATCTTTGACAAACCTGAGAAAAACAAGCAATG 
               
               
                   
                 GGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCT 
               
               
                   
                 AGCCATATGTAGAAAGCTGAAACTGGATCCCTTCCTTACACCTTA 
               
               
                   
                 TACAAAAATCAATTCAAGATGGATTAAAGATTTAAACGTTAAACC 
               
               
                   
                 TAAAACCATAAAAACCCTAGAAGAAAACCTAGGCATTACCATTCA 
               
               
                   
                 GGACATAGGCGTGGGCAAGGACTTCATGTCCAAAACACCAAAAGC 
               
               
                   
                 AATGGCAACAAAAGACAAAATTGACAAATGGGATCTAATTAAACT 
               
               
                   
                 AAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAG 
               
               
                   
                 GCAACCTACAACATGGGAGAAAATTTTTGCAACCTACTCATCTGA 
               
               
                   
                 CAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTA 
               
               
                   
                 CAAGAAAAAAACAAACAACCCCATCAAAAAGTGGGCGAAGGACAT 
               
               
                   
                 GAACAGACACTTCTCAAAAGAAGACATTTATGCAGCCAAAAAACA 
               
               
                   
                 CATGAAGAAATGCTCATCATCACTGGCCATCAGAGAAATGCAAAT 
               
               
                   
                 CAAAACCACTATGAGATATCATCTCACACCAGTTAGAATGGCAAT 
               
               
                   
                 CATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGCGGAGA 
               
               
                   
                 AATAGGAACACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCA 
               
               
                   
                 ACCATTGTGGAAGTCAGTGTGGCGATTCCTCAGGGATCTAGAACT 
               
               
                   
                 AGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCC 
               
               
                   
                 AAATGAGTATAAATCATGCTGCTATAAAGACACATGCACACGTAT 
               
               
                   
                 GTTTATTGCGGCACTATTCACAATAGCAAAGACTTGGAACCAACC 
               
               
                   
                 CAAATGTCCAACAATGATAGACTGGATTAAGAAAATGTGGCACAT 
               
               
                   
                 ATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCAT 
               
               
                   
                 ATCCTTTGTAGGGACATGGATGAAATTGGAAACCATCATTCTCAG 
               
               
                   
                 TAAACTATCGCAAGAACAAAAAACCAAACACCGCATATTCTCACT 
               
               
                   
                 CATAGGTGGGAATTGAACAATGAGATCACATGGACACAGGAAGGG 
               
               
                   
                 GAATATCACACTCTGGGGACTGTGGTGGGGTCGGGGGAGGGGGGA 
               
               
                   
                 GGGATAGCATTGGGAGATATACCTAATGCTAGATGACACATTAGT 
               
               
                   
                 GGGTGCAGCGCACCAGCATGGCACATGTATACGGATCCGAATTCT 
               
               
                   
                 CGACGGATCGATCCGAACAAACGACCCAACACCCGTGCGTTTTAT 
               
               
                   
                 TCTGTCTTTTTATTGCCGATCCCCTCAGAAGAACTCGTCAAGAAG 
               
               
                   
                 GCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTA 
               
               
                   
                 AAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGC 
               
               
                   
                 AATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCGGCCGC 
               
               
                   
                 TCATGTTCTCGTAGGAGTCGGCGTCCTCTTCGTGGTTAGGTCCAG 
               
               
                   
                 GTTGGCCTCTGATAGACCGCAGCTGAGGAGCGGCGTACAGAATGC 
               
               
                   
                 CTCTCATGTCCTCATAGCTGCCGCTGCCTTGTGGAGGCTTCTCGT 
               
               
                   
                 GCTTCAGTGTCTCGTATGTCTCTTGATTCCGGGTGCTCAGGCCGG 
               
               
                   
                 TGTACACGCCATCAGATTTCTCGTAGCTGGTGATGGCGGCCTTCC 
               
               
                   
                 GCACTTGGATCTTCAGCCGTCTGCAGTACAGGGTGATGACCAGAG 
               
               
                   
                 ACAGCAGCAGGACACCACATGTGCCAGCCAGAGGGGCCCAAATGT 
               
               
                   
                 AGATATCCAGGCCTCTGGTATGCACAGCTCCGCCTGCAGCAGGTC 
               
               
                   
                 TACAGGCTTCAGGTCTGAGAGACAGAGGCTGGCTGGCGATTGTAG 
               
               
                   
                 GAGCTGGTGTAGGTGGTCTAGGAGCGGGTGTTGTTGTAGGCTTGG 
               
               
                   
                 CGGGCAGAAACACGGGCACGAAGTGGCTGAAGTACATGATGCTAT 
               
               
                   
                 TGCTCAGGGCTCCGCTTCCTCCGCCGCCTGATTTGATTTCCAGCT 
               
               
                   
                 TGGTGCCTCCGCCAAATGTCCAAGGGCTCTCGTCGTACTGCTGGC 
               
               
                   
                 AGTAGTAGATGCCGAAGTCCTCGTACTGCAGGCTGCTGATTGTCA 
               
               
                   
                 GGGTGTAGTCGGTGCCAGAGCCGCTGCCAGAAAATCTGCTTGGCA 
               
               
                   
                 CGCCGCTTTCCAGTCTGTTGGCCCGGTAGATCAGTGTCTTAGGGG 
               
               
                   
                 CCTTGCCAGGCTTCTGCTGGAACCAGCTCAGGTAGCTGTTGATGT 
               
               
                   
                 CCTGGCTGGCTCTACAGGTGATGGTCACTCTATCGCCCACAGAGG 
               
               
                   
                 CAGACAGGCTGCTAGGGCTCTGTGTCATCTGGATATCAGAGCCAC 
               
               
                   
                 CACCGCCAGATCCACCGCCACCTGATCCTCCGCCTCCGCTAGAAA 
               
               
                   
                 CTGTCACTGTGGTGCCCTGGCCCCACACATCGAAGTACCAGTCGT 
               
               
                   
                 AGCCTCTTCTGGTGCAGAAGTACACGGCGGTATCCTCGGCTCTCA 
               
               
                   
                 GGCTGTTGATCTGCAGGTAGGCGGTGTTCTTGCTGTCGTCCAGGC 
               
               
                   
                 TGAAGGTGAATCTGCCCTTAAAGCTATCGGCGTAGGTTGGCTCGC 
               
               
                   
                 CGGTGTGGGTATTGATCCAGCCCATCCACTCAAGGCCAGGTGAGT 
               
               
                   
                 CCAGGAGATGTTTCAGCACTGTTGCCTTTAGTCTCGAGGCAACTT 
               
               
                   
                 AGACAACTGAGTATTGATCTGAGCACAGCAGGGTGTGAGCTGTTT 
               
               
                   
                 GAAGATACTGGGGTTGGGGGTGAAGAAACTGCAGAGGACTAACTG 
               
               
                   
                 GGCTGAGACCCAGTGGCAATGTTTTAGGGCCTAAGGAATGCCTCT 
               
               
                   
                 GAAAATCTAGATGGACAACTTTGACTTTGAGAAAAGAGAGGTGGA 
               
               
                   
                 AATGAGGAAAATGACTTTTCTTTATTAGATTTCGGTAGAAAGAAC 
               
               
                   
                 TTTCATCTTTCCCCTATTTTTGTTATTCGTTTTAAAACATCTATC 
               
               
                   
                 TGGAGGCAGGACAAGTATGGTCATTAAAAAGATGCAGGCAGAAGG 
               
               
                   
                 CATATATTGGCTCAGTCAAAGTGGGGAACTTTGGTGGCCAAACAT 
               
               
                   
                 ACATTGCTAAGGCTATTCCTATATCAGCTGGACACATATAAAATG 
               
               
                   
                 CTGCTAATGCTTCATTACAAACTTATATCCTTTAATTCCAGATGG 
               
               
                   
                 GGGCAAAGTATGTCCAGGGGTGAGGAACAATTGAAACATTTGGGC 
               
               
                   
                 TGGAGTAGATTTTGAAAGTCAGCTCTGTGTGTGTGTGTGTGTGTG 
               
               
                   
                 TGTGTGTGAGAGCGTGTGTTTCTTTTAACGTTTTCAGCCTACAGC 
               
               
                   
                 ATACAGGGTTCATGGTGGCAAGAAGATAACAAGATTTAAATTATG 
               
               
                   
                 GCCAGTGACTAGTGCTGCAAGAAGAACAACTACCTGCATTTAATG 
               
               
                   
                 GGAAAGCAAAATCTCAGGCTTTGAGGGAAGTTAACATAGGCTTGA 
               
               
                   
                 TTCTGGGTGGAAGCTGGGTGTGTAGTTATCTGGAGGCCAGGCTGG 
               
               
                   
                 AGCTCTCAGCTCACTATGGGTTCATCTTTATTGTCTCCTTTTTCC 
               
               
                   
                 AGGGGCCTGTCGGACCCAGTTCATGCCGTAGTTGGTGAAGGTGTA 
               
               
                   
                 GCCGCTGGCGGCACAGCTGATTCTGACAGATCCGCCAGGTTTCAC 
               
               
                   
                 AAGTCCGCCGCCAGACTGAACCAGCTGGATCTCAGAGATGCTACA 
               
               
                   
                 GGCCACTGTTCCCAGCAGCAGCAGAGACTGCAGCCACATCTGGTG 
               
               
                   
                 GCGAATTCGAAGCTTGAGCTCGAGATCTGAGTCCGGTAGCTGAAC 
               
               
                   
                 CGTCAGATCGCCGGCTAGCGGATCTGACGGTTCACTAAACCAGCT 
               
               
                   
                 CTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGC 
               
               
                   
                 GTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGA 
               
               
                   
                 TTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGA 
               
               
                   
                 CTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATG 
               
               
                   
                 TACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACG 
               
               
                   
                 TAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGG 
               
               
                   
                 GCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGG 
               
               
                   
                 GGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCA 
               
               
                   
                 GTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCT 
               
               
                   
                 ATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGG 
               
               
                   
                 CGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGT 
               
               
                   
                 TATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCC 
               
               
                   
                 GTAATTGATTACTATTAGCCCGGGGGATCCAGACATGATAAGATA 
               
               
                   
                 CATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAA 
               
               
                   
                 ATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAAC 
               
               
                   
                 CATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCA 
               
               
                   
                 TTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG 
               
               
                   
                 CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCGGC 
               
               
                   
                 TGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATG 
               
               
                   
                 CAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGG 
               
               
                   
                 AGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGT 
               
               
                   
                 CGGGGCGCAGCCATGAGGTCGATCGACTCTAGAGGATCGATCCCC 
               
               
                   
                 GCCCCGGACGAACTAAACCTGACTACGACATCTCTGCCCCTTCTT 
               
               
                   
                 CGCGGGGCAGTGCATGTAATCCCTTCAGTTGGTTGGTACAACTTG 
               
               
                   
                 CCAACTGGGCCCTGTTCCACATGTGACACGGGGGGGGACCAAACA 
               
               
                   
                 CAAAGGGGTTCTCTGACTGTAGTTGACATCCTTATAAATGGATGT 
               
               
                   
                 GCACATTTGCCAACACTGAGTGGCTTTCATCCTGGAGCAGACTTT 
               
               
                   
                 GCAGTCTGTGGACTGCAACACAACATTGCCTTTATGTGTAACTCT 
               
               
                   
                 TGGCTGAAGCTCTTACACCAATGCTGGGGGACATGTACCTCCCAG 
               
               
                   
                 GGGCCCAGGAAGACTACGGGAGGCTACACCAACGTCAATCAGAGG 
               
               
                   
                 GGCCTGTGTAGCTACCGATAAGCGGACCCTCAAGAGGGCATTAGC 
               
               
                   
                 AATAGTGTTTATAAGGCCCCCTTGTTAACCCTAAACGGGTAGCAT 
               
               
                   
                 ATGCTTCCCGGGTAGTAGTATATACTATCCAGACTAACCCTAATT 
               
               
                   
                 CAATAGCATATGTTACCCAACGGGAAGCATATGCTATCGAATTAG 
               
               
                   
                 GGTTAGTAAAAGGGTCCTAAGGAACAGCGATATCTCCCACCCCAT 
               
               
                   
                 GAGCTGTCACGGTTTTATTTACATGGGGTCAGGATTCCACGAGGG 
               
               
                   
                 TAGTGAACCATTTTAGTCACAAGGGCAGTGGCTGAAGATCAAGGA 
               
               
                   
                 GCGGGCAGTGAACTCTCCTGAATCTTCGCCTGCTTCTTCATTCTC 
               
               
                   
                 CTTCGTTTAGCTAATAGAATAACTGCTGAGTTGTGAACAGTAAGG 
               
               
                   
                 TGTATGTGAGGTGCTCGAAAACAAGGTTTCAGGTGACGCCCCCAG 
               
               
                   
                 AATAAAATTTGGACGGGGGGTTCAGTGGTGGCATTGTGCTATGAC 
               
               
                   
                 ACCAATATAACCCTCACAAACCCCTTGGGCAATAAATACTAGTGT 
               
               
                   
                 AGGAATGAAACATTCTGAATATCTTTAACAATAGAAATCCATGGG 
               
               
                   
                 GTGGGGACAAGCCGTAAAGACTGGATGTCCATCTCACACGAATTT 
               
               
                   
                 ATGGCTATGGGCAACACATAATCCTAGTGCAATATGATACTGGGG 
               
               
                   
                 TTATTAAGATGTGTCCCAGGCAGGGACCAAGACAGGTGAACCATG 
               
               
                   
                 TTGTTACACTCTATTTGTAACAAGGGGAAAGAGAGTGGACGCCGA 
               
               
                   
                 CAGCAGCGGACTCCACTGGTTGTCTCTAACACCCCCGAAAATTAA 
               
               
                   
                 ACGGGGCTCCACGCCAATGGGGCCCATAAACAAAGACAAGTGGCC 
               
               
                   
                 ACTCTTTTTTTTGAAATTGTGGAGTGGGGGCACGCGTCAGCCCCC 
               
               
                   
                 ACACGCCGCCCTGCGGTTTTGGACTGTAAAATAAGGGTGTAATAA 
               
               
                   
                 CTTGGCTGATTGTAACCCCGCTAACCACTGCGGTCAAACCACTTG 
               
               
                   
                 CCCACAAAACCACTAATGGCACCCCGGGGAATACCTGCATAAGTA 
               
               
                   
                 GGTGGGCGGGCCAAGATAGGGGCGCGATTGCTGCGATCTGGAGGA 
               
               
                   
                 CAAATTACACACACTTGCGCCTGAGCGCCAAGCACAGGGTTGTTG 
               
               
                   
                 GTCCTCATATTCACGAGGTCGCTGAGAGCACGGTGGGCTAATGTT 
               
               
                   
                 GCCATGGGTAGCATATACTACCCAAATATCTGGATAGCATATGCT 
               
               
                   
                 ATCCTAATCTATATCTGGGTAGCATAGGCTATCCTAATCTATATC 
               
               
                   
                 TGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGCT 
               
               
                   
                 ATCCTAATTTATATCTGGGTAGCATAGGCTATCCTAATCTATATC 
               
               
                   
                 TGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGCT 
               
               
                   
                 ATCCTAATCTGTATCCGGGTAGCATATGCTATCCTAATAGAGATT 
               
               
                   
                 AGGGTAGTATATGCTATCCTAATTTATATCTGGGTAGCATATACT 
               
               
                   
                 ACCCAAATATCTGGATAGCATATGCTATCCTAATCTATATCTGGG 
               
               
                   
                 TAGCATATGCTATCCTAATCTATATCTGGGTAGCATAGGCTATCC 
               
               
                   
                 TAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGG 
               
               
                   
                 TAGTATATGCTATCCTAATTTATATCTGGGTAGCATAGGCTATCC 
               
               
                   
                 TAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGG 
               
               
                   
                 TAGTATATGCTATCCTAATCTGTATCCGGGTAGCATATGCTATCC 
               
               
                   
                 TCATGCATATACAGTCAGCATATGATACCCAGTAGTAGAGTGGGA 
               
               
                   
                 GTGCTATCCTTTGCATATGCCGCCACCTCCCAAGGGGGCGTGAAT 
               
               
                   
                 TTTCGCTGCTTGTCCTTTTCCTGCATGCTGGTTGCTCCCATTCTT 
               
               
                   
                 AGGTGAATTTAAGGAGGCCAGGCTAAAGCCGTCGCATGTCTGATT 
               
               
                   
                 GCTCACCAGGTAAATGTCGCTAATGTTTTCCAACGCGAGAAGGTG 
               
               
                   
                 TTGAGCGCGGAGCTGAGTGACGTGACAACATGGGTATGCCCAATT 
               
               
                   
                 GCCCCATGTTGGGAGGACGAAAATGGTGACAAGACAGATGGCCAG 
               
               
                   
                 AAATACACCAACAGCACGCATGATGTCTACTGGGGATTTATTCTT 
               
               
                   
                 TAGTGCGGGGGAATACACGGCTTTTAATACGATTGAGGGCGTCTC 
               
               
                   
                 CTAACAAGTTACATCACTCCTGCCCTTCCTCACCCTCATCTCCAT 
               
               
                   
                 CACCTCCTTCATCTCCGTCATCTCCGTCATCACCCTCCGCGGCAG 
               
               
                   
                 CCCCTTCCACCATAGGTGGAAACCAGGGAGGCAAATCTACTCCAT 
               
               
                   
                 CGTCAAAGCTGCACACAGTCACCCTGATATTGCAGGTAGGAGCGG 
               
               
                   
                 GCTTTGTCATAACAAGGTCCTTAATCGCATCCTTCAAAACCTCAG 
               
               
                   
                 CAAATATATGAGTTTGTAAAAAGACCATGAAATAACAGACAATGG 
               
               
                   
                 ACTCCCTTAGCGGGCCAGGTTGTGGGCCGGGTCCAGGGGCCATTC 
               
               
                   
                 CAAAGGGGAGACGACTCAATGGTGTAAGACGACATTGTGGAATAG 
               
               
                   
                 CAAGGGCAGTTCCTCGCCTTAGGTTGTAAAGGGAGGTCTTACTAC 
               
               
                   
                 CTCCATATACGAACACACCGGCGACCCAAGTTCCTTCGTCGGTAG 
               
               
                   
                 TCCTTTCTACGTGACTCCTAGCCAGGAGAGCTCTTAAACCTTCTG 
               
               
                   
                 CAATGTTCTCAAATTTCGGGTTGGAACCTCCTTGACCACGATGCT 
               
               
                   
                 TTCCAAACCACCCTCCTTTTTTGCGCCTGCCTCCATCACCCTGAC 
               
               
                   
                 CCCGGGGTCCAGTGCTTGGGCCTTCTCCTGGGTCATCTGCGGGGC 
               
               
                   
                 CCTGCTCTATCGCTCCCGGGGGCACGTCAGGCTCACCATCTGGGC 
               
               
                   
                 CACCTTCTTGGTGGTATTCAAAATAATCGGCTTCCCCTACAGGGT 
               
               
                   
                 GGAAAAATGGCCTTCTACCTGGAGGGGGCCTGCGCGGTGGAGACC 
               
               
                   
                 CGGATGATGATGACTGACTACTGGGACTCCTGGGCCTCTTTTCTC 
               
               
                   
                 CACGTCCACGACCTCTCCCCCTGGCTCTTTCACGACTTCCCCCCC 
               
               
                   
                 TGGCTCTTTCACGTCCTCTACCCCGGCGGCCTCCACTACCTCCTC 
               
               
                   
                 GACCCCGGCCTCCACTACCTCCTCGACCCCGGCCTCCACTGCCTC 
               
               
                   
                 CTCGACCCCGGCCTCCACCTCCTGCTCCTGCCCCTCCTGCTCCTG 
               
               
                   
                 CCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCC 
               
               
                   
                 CTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTG 
               
               
                   
                 CCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTC 
               
               
                   
                 CTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCC 
               
               
                   
                 CTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTG 
               
               
                   
                 CTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCCTG 
               
               
                   
                 CCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCTCCTGCCC 
               
               
                   
                 CTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTCCTGCTC 
               
               
                   
                 CTGCCCCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTC 
               
               
                   
                 CTGCCCCTCCTCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTC 
               
               
                   
                 CTGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTC 
               
               
                   
                 CTCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTC 
               
               
                   
                 CTGCCCCTCCTCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTC 
               
               
                   
                 CTGCCCCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTC 
               
               
                   
                 CTCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCCCGCTCCTGCTC 
               
               
                   
                 CTGCTCCTGTTCCACCGTGGGTCCCTTTGCAGCCAATGCAACTTG 
               
               
                   
                 GACGTTTTTGGGGTCTCCGGACACCATCTCTATGTCTTGGCCCTG 
               
               
                   
                 ATCCTGAGCCGCCCGGGGCTCCTGGTCTTCCGCCTCCTCGTCCTC 
               
               
                   
                 GTCCTCTTCCCCGTCCTCGTCCATGGTTATCACCCCCTCTTCTTT 
               
               
                   
                 GAGGTCCACTGCCGCCGGAGCCTTCTGGTCCAGATGTGTCTCCCT 
               
               
                   
                 TCTCTCCTAGGCCATTTCCAGGTCCTGTACCTGGCCCCTCGTCAG 
               
               
                   
                 ACATGATTCACACTAAAAGAGATCAATAGACATCTTTATTAGACG 
               
               
                   
                 ACGCTCAGTGAATACAGGGAGTGCAGACTCCTGCCCCCTCCAACA 
               
               
                   
                 GCCCCCCCACCCTCATCCCCTTCATGGTCGCTGTCAGACAGATCC 
               
               
                   
                 AGGTCTGAAAATTCCCCATCCTCCGAACCATCCTCGTCCTCATCA 
               
               
                   
                 CCAATTACTCGCAGCCCGGAAAACTCCCGCTGAACATCCTCAAGA 
               
               
                   
                 TTTGCGTCCTGAGCCTCAAGCCAGGCCTCAAATTCCTCGTCCCCC 
               
               
                   
                 TTTTTGCTGGACGGTAGGGATGGGGATTCTCGGGACCCCTCCTCT 
               
               
                   
                 TCCTCTTCAAGGTCACCAGACAGAGATGCTACTGGGGCAACGGAA 
               
               
                   
                 GAAAAGCTGGGTGCGGCCTGTGAGGATCAGCTTATCGATGATAAG 
               
               
                   
                 CTGTCAAACATGAGAATTCTTGAAGACGAAAGGGCCTCGTGATAC 
               
               
                   
                 GCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGA 
               
               
                   
                 CGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTT 
               
               
                   
                 GTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGAC 
               
               
                   
                 AATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTA 
               
               
                   
                 TGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG 
               
               
                   
                 CATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAG 
               
               
                   
                 TAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCG 
               
               
                   
                 AACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCG 
               
               
                   
                 AAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTG 
               
               
                   
                 GCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTC 
               
               
                   
                 GCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAG 
               
               
                   
                 TCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTAT 
               
               
                   
                 GCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTAC 
               
               
                   
                 TTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGC 
               
               
                   
                 ACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG 
               
               
                   
                 AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGC 
               
               
                   
                 CTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAAC 
               
               
                   
                 TACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGG 
               
               
                   
                 CGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTG 
               
               
                   
                 GCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTC 
               
               
                   
                 GCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTA 
               
               
                   
                 TCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAC 
               
               
                   
                 GAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATT 
               
               
                   
                 GGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATT 
               
               
                   
                 TAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTT 
               
               
                   
                 TTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCC 
               
               
                   
                 ACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAG 
               
               
                   
                 ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAC 
               
               
                   
                 CACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAA 
               
               
                   
                 CTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAA 
               
               
                   
                 ATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA 
               
               
                   
                 ACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAC 
               
               
                   
                 CAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGG 
               
               
                   
                 ACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAA 
               
               
                   
                 CGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGC 
               
               
                   
                 TTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGG 
               
               
                   
                 TCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCT 
               
               
                   
                 GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGC 
               
               
                   
                 GTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAA 
               
               
                   
                 ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGC 
               
               
                   
                 CTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGG 
               
               
                   
                 ATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCA 
               
               
                   
                 GCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG 
               
               
                   
                 AGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTT 
               
               
                   
                 CACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCG 
               
               
                   
                 CATAGTTAAGCCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAA 
               
               
                   
                 AGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATC 
               
               
                   
                 TCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAG 
               
               
                   
                 CAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCA 
               
               
                   
                 TAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCA 
               
               
                   
                 GTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTT 
               
               
                   
                 ATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGT 
               
               
                   
                 AGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTG 
               
               
                   
                 CATGCCTGCAGGTCGGCCGCCACGACCGGTGCCGCCACCATCCCC 
               
               
                   
                 TGACCCACGCCCCTGACCCCTCACAAGGAGACGACCTTCCATGAC 
               
               
                   
                 CGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCC 
               
               
                   
                 CCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGC 
               
               
                   
                 CACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTCAC 
               
               
                   
                 CGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGG 
               
               
                   
                 CAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGAC 
               
               
                   
                 CACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGG 
               
               
                   
                 CCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCA 
               
               
                   
                 ACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGC 
               
               
                   
                 GTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAA 
               
               
                   
                 GGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGA 
               
               
                   
                 GCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAA 
               
               
                   
                 CCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGT 
               
               
                   
                 CGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCC 
               
               
                   
                 CGGTGCCTGACGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAG 
               
               
                   
                 GAGCGCACGACCCCATGGCTCCGACCGAAGCCGACCCGGGCGGCC 
               
               
                   
                 CCGCCGACCCCGCACCCGCCCCCGAGGCCCACCGACTCTAGAGGA 
               
               
                   
                 TCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAA 
               
               
                   
                 AAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATG 
               
               
                   
                 CAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACA 
               
               
                   
                 AATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTT 
               
               
                   
                 CACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTT 
               
               
                   
                 ATCATGTCTGGATCACTCGCCGATAGTGGAAACCGACGCCCCAGC 
               
               
                   
                 ACTCGTCCGAGGGCAAAGGAATAGGGGAGATGGGGGAGGCTAACT 
               
               
                   
                 GAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACG 
               
               
                   
                 GCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGT 
               
               
                   
                 TCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATAC 
               
               
                   
                 CCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTT 
               
               
                   
                 TTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGC 
               
               
                   
                 AGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCCACTGGCCCCGT 
               
               
                   
                 GGGTTAGGGACGGGGTCCCCCATGGGGAATGGTTTATGGTTCGTG 
               
               
                   
                 GGGGTTATTATTTTGGGCGTTGCGTGGGGTCTGGTCCACGACTGG 
               
               
                   
                 ACTGAGCAGACAGACCCATGGTTTTTGGATGGCCTGGGCATGGAC 
               
               
                   
                 CGCATGTACTGGCGCGACACGAACACCGGGCGTCTGTGGCTGCCA 
               
               
                   
                 AACACCCCCGACCCCCAAAAACCACCGCGCGGATTTCTGGCGTGC 
               
               
                   
                 CAAGCTAGTCGACCAATTCTCATGTTTGACAGCTTATCATCGCAG 
               
               
                   
                 ATCCGGGCAACGTTGTTGCATTGCTGCAGGCGCAGAACTGGTAGG 
               
               
                   
                 TATGGAAGATCTCTAGAAGCTGGGTACCAGCTGCTAGCAAGCTTG 
               
               
                   
                 CTAGCGGCCGGCTCGAGTTTACTCCCTATCAGTGATAGAGAACGT 
               
               
                   
                 ATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGATGTCGAGTT 
               
               
                   
                 TACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTA 
               
               
                   
                 TCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATA 
               
               
                   
                 GAGAACGTATGTCGAGTTTATCCCTATCAGTGATAGAGAACGTAT 
               
               
                   
                 GTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGGTA 
               
               
                   
                 GGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTCGTTTAG 
               
               
                   
                 (SEQ ID NO: 39) 
               
               
                   
                   
               
               
                   
                 LINE-1 plasmid-CD5_FCR-PI3K_T2A-GFP intron 
               
               
                   
                 (SEQ ID NO: 40) 
               
               
                   
                 CGGCCGCGGGGGGAGGAGCCAAGATGGCCGAATAGGAACAGCTCC 
               
               
                   
                 GGTCTACAGCTCCCAGCGTGAGCGACGCAGAAGACGGTGATTTCT 
               
               
                   
                 GCATTTCCATCTGAGGTACCGGGTTCATCTCACTAGGGAGTGCCA 
               
               
                   
                 GACAGTGGGCGCAGGCCAGTGTGTGTGCGCACCGTGCGCGAGCCG 
               
               
                   
                 AAGCAGGGCGAGGCATTGCCTCACCTGGGAAGCGCAAGGGGTCAG 
               
               
                   
                 GGAGTTCCCTTTCCGAGTCAAAGAAAGGGGTGACGGACGCACCTG 
               
               
                   
                 GAAAATCGGGTCACTCCCACCCGAATATTGCGCTTTTCAGACCGG 
               
               
                   
                 CTTAAGAAACGGCGCACCACGAGACTATATCCCACACCTGGCTCG 
               
               
                   
                 GAGGGTCCTACGCCCACGGAATCTCGCTGATTGCTAGCACAGCAG 
               
               
                   
                 TCTGAGATCAAACTGCAAGGCGGCAACGAGGCTGGGGGAGGGGCG 
               
               
                   
                 CCCGCCATTGCCCAGGCTTGCTTAGGTAAACAAAGCAGCAGGGAA 
               
               
                   
                 GCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTG 
               
               
                   
                 CCTCTGTAGGCTCCACCTCTGGGGGCAGGGCACAGACAAACAAAA 
               
               
                   
                 AGACAGCAGTAACCTCTGCAGACTTAAGTGTCCCTGTCTGACAGC 
               
               
                   
                 TTTGAAGAGAGCAGTGGTTCTCCCAGCACGCAGCTGGAGATCTGA 
               
               
                   
                 GAACGGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCTGACCC 
               
               
                   
                 CCGAGCAGCCTAACTGGGAGGCACCCCCCAGCAGGGGCACACTGA 
               
               
                   
                 CACCTCACACGGCAGGGTATTCCAACAGACCTGCAGCTGAGGGTC 
               
               
                   
                 CTGTCTGTTAGAAGGAAAACTAACAACCAGAAAGGACATCTACAC 
               
               
                   
                 CGAAAACCCATCTGTACATCACCATCATCAAAGACCAAAAGTAGA 
               
               
                   
                 TAAAACCACAAAGATGGGGAAAAAACAGAACAGAAAAACTGGAAA 
               
               
                   
                 CTCTAAAACGCAGAGCGCCTCTCCTCCTCCAAAGGAACGCAGTTC 
               
               
                   
                 CTCACCAGCAACAGAACAAAGCTGGATGGAGAATGATTTTGATGA 
               
               
                   
                 GCTGAGAGAAGAAGGCTTCAGACGATCAAATTACTCTGAGCTACG 
               
               
                   
                 GGAGGACATTCAAACCAAAGGCAAAGAAGTTGAAAACTTTGAAAA 
               
               
                   
                 AAATTTAGAAGAATGTATAACTAGAATAACCAATACAGAGAAGTG 
               
               
                   
                 CTTAAAGGAGCTGATGGAGCTGAAAACCAAGGCTCGAGAACTACG 
               
               
                   
                 TGAAGAATGCAGAAGCCTCAGGAGCCGATGCGATCAACTGGAAGA 
               
               
                   
                 AAGGGTATCAGCAATGGAAGATGAAATGAATGAAATGAAGCGAGA 
               
               
                   
                 AGGGAAGTTTAGAGAAAAAAGAATAAAAAGAAATGAGCAAAGCCT 
               
               
                   
                 CCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACGTCTGAT 
               
               
                   
                 TGGTGTACCTGAAAGTGATGTGGAGAATGGAACCAAGTTGGAAAA 
               
               
                   
                 CACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAG 
               
               
                   
                 GCAGGCCAACGTTCAGATTCAGGAAATACAGAGAACGCCACAAAG 
               
               
                   
                 ATACTCCTCGAGAAGAGCAACTCCAAGACACATAATTGTCAGATT 
               
               
                   
                 CACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGA 
               
               
                   
                 GAAAGGTCGGGTTACCCTCAAAGGAAAGCCCATCAGACTAACAGC 
               
               
                   
                 GGATCTCTCGGCAGAAACCCTACAAGCCAGAAGAGAGTGGGGGCC 
               
               
                   
                 AATATTCAACATTCTTAAAGAAAAGAATTTTCAACCCAGAATTTC 
               
               
                   
                 ATATCCAGCCAAACTAAGCTTCATAAGTGAAGGAGAAATAAAATA 
               
               
                   
                 CTTTATAGACAAGCAAATGTTGAGAGATTTTGTCACCACCAGGCC 
               
               
                   
                 TGCCCTAAAAGAGCTCCTGAAGGAAGCGCTAAACATGGAAAGGAA 
               
               
                   
                 CAACCGGTACCAGCCGCTGCAAAATCATGCCAAAATGTAAAGACC 
               
               
                   
                 ATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACC 
               
               
                   
                 AGCTAACATCATAATGACAGGATCAACTTCACACATAACAATATT 
               
               
                   
                 AACTTTAAATATAAATGGACTAAATTCTGCAATTAAAAGACACAG 
               
               
                   
                 ACTGGCAAGTTGGATAAAGAGTCAAGACCCATCAGTGTGCTGTAT 
               
               
                   
                 TCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCAAAAT 
               
               
                   
                 AAAAGGATGGAGGAAGATCTACCAAGCCAATGGAAAACAAAAAAA 
               
               
                   
                 GGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGACTTTAAACC 
               
               
                   
                 AACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGTAAA 
               
               
                   
                 GGGATCAATTCAACAAGAGGAGCTAACTATCCTAAATATTTATGC 
               
               
                   
                 ACCCAATACAGGAGCACCCAGATTCATAAAGCAAGTCCTCAGTGA 
               
               
                   
                 CCTACAAAGAGACTTAGACTCCCACACATTAATAATGGGAGACTT 
               
               
                   
                 TAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGT 
               
               
                   
                 CAACAAGGATACCCAGGAATTGAACTCAGCTCTGCACCAAGCAGA 
               
               
                   
                 CCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATA 
               
               
                   
                 TACATTTTTTTCAGCACCACACCACACCTATTCCAAAATTGACCA 
               
               
                   
                 CATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGA 
               
               
                   
                 AATTATAACAAACTATCTCTCAGACCACAGTGCAATCAAACTAGA 
               
               
                   
                 ACTCAGGATTAAGAATCTCACTCAAAGCCGCTCAACTACATGGAA 
               
               
                   
                 ACTGAACAACCTGCTCCTGAATGACTACTGGGTACATAACGAAAT 
               
               
                   
                 GAAGGCAGAAATAAAGATGTTCTTTGAAACCAACGAGAACAAAGA 
               
               
                   
                 CACCACATACCAGAATCTCTGGGACGCATTCAAAGCAGTGTGTAG 
               
               
                   
                 AGGGAAATTTATAGCACTAAATGCCTACAAGAGAAAGCAGGAAAG 
               
               
                   
                 ATCCAAAATTGACACCCTAACATCACAATTAAAAGAACTAGAAAA 
               
               
                   
                 GCAAGAGCAAACACATTCAAAAGCTAGCAGAAGGCAAGAAATAAC 
               
               
                   
                 TAAAATCAGAGCAGAACTGAAGGAAATAGAGACACAAAAAACCCT 
               
               
                   
                 TCAAAAAATCAATGAATCCAGGAGCTGGTTTTTTGAAAGGATCAA 
               
               
                   
                 CAAAATTGATAGACCGCTAGCAAGACTAATAAAGAAAAAAAGAGA 
               
               
                   
                 GAAGAATCAAATAGACACAATAAAAAATGATAAAGGGGATATCAC 
               
               
                   
                 CACCGATCCCACAGAAATACAAACTACCATCAGAGAATACTACAA 
               
               
                   
                 ACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAC 
               
               
                   
                 ATTCCTCGACACATACACTCTCCCAAGACTAAACCAGGAAGAAGT 
               
               
                   
                 TGAATCTCTGAATCGACCAATAACAGGCTCTGAAATTGTGGCAAT 
               
               
                   
                 AATCAATAGTTTACCAACCAAAAAGAGTCCAGGACCAGATGGATT 
               
               
                   
                 CACAGCCGAATTCTACCAGAGGTACAAGGAGGAACTGGTACCATT 
               
               
                   
                 CCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCC 
               
               
                   
                 TAACTCATTTTATGAGGCCAGCATCATTCTGATACCAAAGCCGGG 
               
               
                   
                 CAGAGACACAACCAAAAAAGAGAATTTTAGACCAATATCCTTGAT 
               
               
                   
                 GAACATTGATGCAAAAATCCTCAATAAAATACTGGCAAACCGAAT 
               
               
                   
                 CCAGCAGCACATCAAAAAGCTTATCCACCATGATCAAGTGGGCTT 
               
               
                   
                 CATCCCTGGGATGCAAGGCTGGTTCAATATACGCAAATCAATAAA 
               
               
                   
                 TGTAATCCAGCATATAAACAGAGCCAAAGACAAAAACCACATGAT 
               
               
                   
                 TATCTCAATAGATGCAGAAAAAGCCTTTGACAAAATTCAACAACC 
               
               
                   
                 CTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACGTA 
               
               
                   
                 TTTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATAT 
               
               
                   
                 CATACTGAATGGGCAAAAACTGGAAGCATTCCCTTTGAAAACCGG 
               
               
                   
                 CACAAGACAGGGATGCCCTCTCTCACCGCTCCTATTCAACATAGT 
               
               
                   
                 GTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAA 
               
               
                   
                 GGGTATTCAATTAGGAAAAGAGGAAGTCAAATTGTCCCTGTTTGC 
               
               
                   
                 AGACGACATGATTGTTTATCTAGAAAACCCCATCGTCTCAGCCCA 
               
               
                   
                 AAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATA 
               
               
                   
                 CAAAATCAATGTACAAAAATCACAAGCATTCTTATACACCAACAA 
               
               
                   
                 CAGACAAACAGAGAGCCAAATCATGGGTGAACTCCCATTCACAAT 
               
               
                   
                 TGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGA 
               
               
                   
                 TGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAAGGA 
               
               
                   
                 AATAAAAGAGGAGACAAACAAATGGAAGAACATTCCATGCTCATG 
               
               
                   
                 GGTAGGAAGAATCAATATCGTGAAAATGGCCATACTGCCCAAGGT 
               
               
                   
                 AATTTACAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTT 
               
               
                   
                 CTTCACAGAATTGGAAAAAACTACTTTAAAGTTCATATGGAACCA 
               
               
                   
                 AAAAAGAGCCCGCATTGCCAAGTCAATCCTAAGCCAAAAGAACAA 
               
               
                   
                 AGCTGGAGGCATCACACTACCTGACTTCAAACTATACTACAAGGC 
               
               
                   
                 TACAGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATAT 
               
               
                   
                 AGATCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATAT 
               
               
                   
                 CTACAACTATCTGATCTTTGACAAACCTGAGAAAAACAAGCAATG 
               
               
                   
                 GGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCT 
               
               
                   
                 AGCCATATGTAGAAAGCTGAAACTGGATCCCTTCCTTACACCTTA 
               
               
                   
                 TACAAAAATCAATTCAAGATGGATTAAAGATTTAAACGTTAAACC 
               
               
                   
                 TAAAACCATAAAAACCCTAGAAGAAAACCTAGGCATTACCATTCA 
               
               
                   
                 GGACATAGGCGTGGGCAAGGACTTCATGTCCAAAACACCAAAAGC 
               
               
                   
                 AATGGCAACAAAAGACAAAATTGACAAATGGGATCTAATTAAACT 
               
               
                   
                 AAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAG 
               
               
                   
                 GCAACCTACAACATGGGAGAAAATTTTTGCAACCTACTCATCTGA 
               
               
                   
                 CAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTA 
               
               
                   
                 CAAGAAAAAAACAAACAACCCCATCAAAAAGTGGGCGAAGGACAT 
               
               
                   
                 GAACAGACACTTCTCAAAAGAAGACATTTATGCAGCCAAAAAACA 
               
               
                   
                 CATGAAGAAATGCTCATCATCACTGGCCATCAGAGAAATGCAAAT 
               
               
                   
                 CAAAACCACTATGAGATATCATCTCACACCAGTTAGAATGGCAAT 
               
               
                   
                 CATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGCGGAGA 
               
               
                   
                 AATAGGAACACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCA 
               
               
                   
                 ACCATTGTGGAAGTCAGTGTGGCGATTCCTCAGGGATCTAGAACT 
               
               
                   
                 AGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCC 
               
               
                   
                 AAATGAGTATAAATCATGCTGCTATAAAGACACATGCACACGTAT 
               
               
                   
                 GTTTATTGCGGCACTATTCACAATAGCAAAGACTTGGAACCAACC 
               
               
                   
                 CAAATGTCCAACAATGATAGACTGGATTAAGAAAATGTGGCACAT 
               
               
                   
                 ATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCAT 
               
               
                   
                 ATCCTTTGTAGGGACATGGATGAAATTGGAAACCATCATTCTCAG 
               
               
                   
                 TAAACTATCGCAAGAACAAAAAACCAAACACCGCATATTCTCACT 
               
               
                   
                 CATAGGTGGGAATTGAACAATGAGATCACATGGACACAGGAAGGG 
               
               
                   
                 GAATATCACACTCTGGGGACTGTGGTGGGGTCGGGGGAGGGGGGA 
               
               
                   
                 GGGATAGCATTGGGAGATATACCTAATGCTAGATGACACATTAGT 
               
               
                   
                 GGGTGCAGCGCACCAGCATGGCACATGTATACGGATCCGAATTCT 
               
               
                   
                 CGACGGATCGATCCGAACAAACGACCCAACACCCGTGCGTTTTAT 
               
               
                   
                 TCTGTCTTTTTATTGCCGATCCCCTCAGAAGAACTCGTCAAGAAG 
               
               
                   
                 GCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTA 
               
               
                   
                 AAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGC 
               
               
                   
                 AATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCGGCCGC 
               
               
                   
                 TTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGG 
               
               
                   
                 TCACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGT 
               
               
                   
                 CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCA 
               
               
                   
                 GCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGT 
               
               
                   
                 CGGCCAGGTGAGTCCAGGAGATGTTTCAGCACTGTTGCCTTTAGT 
               
               
                   
                 CTCGAGGCAACTTAGACAACTGAGTATTGATCTGAGCACAGCAGG 
               
               
                   
                 GTGTGAGCTGTTTGAAGATACTGGGGTTGGGGGTGAAGAAACTGC 
               
               
                   
                 AGAGGACTAACTGGGCTGAGACCCAGTGGCAATGTTTTAGGGCCT 
               
               
                   
                 AAGGAATGCCTCTGAAAATCTAGATGGACAACTTTGACTTTGAGA 
               
               
                   
                 AAAGAGAGGTGGAAATGAGGAAAATGACTTTTCTTTATTAGATTT 
               
               
                   
                 CGGTAGAAAGAACTTTCATCTTTCCCCTATTTTTGTTATTCGTTT 
               
               
                   
                 TAAAACATCTATCTGGAGGCAGGACAAGTATGGTCATTAAAAAGA 
               
               
                   
                 TGCAGGCAGAAGGCATATATTGGCTCAGTCAAAGTGGGGAACTTT 
               
               
                   
                 GGTGGCCAAACATACATTGCTAAGGCTATTCCTATATCAGCTGGA 
               
               
                   
                 CACATATAAAATGCTGCTAATGCTTCATTACAAACTTATATCCTT 
               
               
                   
                 TAATTCCAGATGGGGGCAAAGTATGTCCAGGGGTGAGGAACAATT 
               
               
                   
                 GAAACATTTGGGCTGGAGTAGATTTTGAAAGTCAGCTCTGTGTGT 
               
               
                   
                 GTGTGTGTGTGTGTGTGTGTGAGAGCGTGTGTTTCTTTTAACGTT 
               
               
                   
                 TTCAGCCTACAGCATACAGGGTTCATGGTGGCAAGAAGATAACAA 
               
               
                   
                 GATTTAAATTATGGCCAGTGACTAGTGCTGCAAGAAGAACAACTA 
               
               
                   
                 CCTGCATTTAATGGGAAAGCAAAATCTCAGGCTTTGAGGGAAGTT 
               
               
                   
                 AACATAGGCTTGATTCTGGGTGGAAGCTGGGTGTGTAGTTATCTG 
               
               
                   
                 GAGGCCAGGCTGGAGCTCTCAGCTCACTATGGGTTCATCTTTATT 
               
               
                   
                 GTCTCCTTTCATCTCAACAGCTGCACGCTGCCGTCCTCGATGTTG 
               
               
                   
                 TGGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCG 
               
               
                   
                 GCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTG 
               
               
                   
                 TGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGC 
               
               
                   
                 TCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCG 
               
               
                   
                 CGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCC 
               
               
                   
                 TGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGC 
               
               
                   
                 TTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTC 
               
               
                   
                 AGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTG 
               
               
                   
                 GTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCC 
               
               
                   
                 TCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCG 
               
               
                   
                 TCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCG 
               
               
                   
                 CCCTTGCTCACCATAGGGCCGGGATTCTCCTCCACGTCACCGCAT 
               
               
                   
                 GTTAGAAGACTTCCTCTGCCCTCCATGTTCTCGTAGGAGTCGGCG 
               
               
                   
                 TCCTCTTCGTGGTTAGGTCCAGGTTGGCCTCTGATAGACCGCAGC 
               
               
                   
                 TGAGGAGCGGCGTACAGAATGCCTCTCATGTCCTCATAGCTGCCG 
               
               
                   
                 CTGCCTTGTGGAGGCTTCTCGTGCTTCAGTGTCTCGTATGTCTCT 
               
               
                   
                 TGATTCCGGGTGCTCAGGCCGGTGTACACGCCATCAGATTTCTCG 
               
               
                   
                 TAGCTGGTGATGGCGGCCTTCCGCACTTGGATCTTCAGCCGTCTG 
               
               
                   
                 CAGTACAGGGTGATGACCAGAGACAGCAGCAGGACACCACATGTG 
               
               
                   
                 CCAGCCAGAGGGGCCCAAATGTAGATATCCAGGCCTCTGGTATGC 
               
               
                   
                 ACAGCTCCGCCTGCAGCAGGTCTACAGGCTTCAGGTCTGAGAGAC 
               
               
                   
                 AGAGGCTGGCTGGCGATTGTAGGAGCTGGTGTAGGTGGTCTAGGA 
               
               
                   
                 GCGGGTGTTGTTGTAGGCTTGGCGGGCAGAAACACGGGCACGAAG 
               
               
                   
                 TGGCTGAAGTACATGATGCTATTGCTCAGGGCTCCGCTTCCTCCG 
               
               
                   
                 CCGCCTGATTTGATTTCCAGCTTGGTGCCTCCGCCAAATGTCCAA 
               
               
                   
                 GGGCTCTCGTCGTACTGCTGGCAGTAGTAGATGCCGAAGTCCTCG 
               
               
                   
                 TACTGCAGGCTGCTGATTGTCAGGGTGTAGTCGGTGCCAGAGCCG 
               
               
                   
                 CTGCCAGAAAATCTGCTTGGCACGCCGCTTTCCAGTCTGTTGGCC 
               
               
                   
                 CGGTAGATCAGTGTCTTAGGGGCCTTGCCAGGCTTCTGCTGGAAC 
               
               
                   
                 CAGCTCAGGTAGCTGTTGATGTCCTGGCTGGCTCTACAGGTGATG 
               
               
                   
                 GTCACTCTATCGCCCACAGAGGCAGACAGGCTGCTAGGGCTCTGT 
               
               
                   
                 GTCATCTGGATATCAGAGCCACCACCGCCAGATCCACCGCCACCT 
               
               
                   
                 GATCCTCCGCCTCCGCTAGAAACTGTCACTGTGGTGCCCTGGCCC 
               
               
                   
                 CACACATCGAAGTACCAGTCGTAGCCTCTTCTGGTGCAGAAGTAC 
               
               
                   
                 ACGGCGGTATCCTCGGCTCTCAGGCTGTTGATCTGCAGGTAGGCG 
               
               
                   
                 GTGTTCTTGCTGTCGTCCAGGCTGAAGGTGAATCTGCCCTTAAAG 
               
               
                   
                 CTATCGGCGTAGGTTGGCTCGCCGGTGTGGGTATTGATCCAGCCC 
               
               
                   
                 ATCCACTCAAGGCCTTTTCCAGGGGCCTGTCGGACCCAGTTCATG 
               
               
                   
                 CCGTAGTTGGTGAAGGTGTAGCCGCTGGCGGCACAGCTGATTCTG 
               
               
                   
                 ACAGATCCGCCAGGTTTCACAAGTCCGCCGCCAGACTGAACCAGC 
               
               
                   
                 TGGATCTCAGAGATGCTACAGGCCACTGTTCCCAGCAGCAGCAGA 
               
               
                   
                 GACTGCAGCCACATTCGAAGCTTGAGCTCGAGATCTGAGTCCGGT 
               
               
                   
                 AGCGCTAGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATAT 
               
               
                   
                 AGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGG 
               
               
                   
                 CGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATC 
               
               
                   
                 CCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAA 
               
               
                   
                 ACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACT 
               
               
                   
                 GCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCC 
               
               
                   
                 AGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTG 
               
               
                   
                 GCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTA 
               
               
                   
                 AATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTA 
               
               
                   
                 CTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGT 
               
               
                   
                 TGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGC 
               
               
                   
                 GGAACTCCATATATGGGCTATGAACTAATGACCCCGTAATTGATT 
               
               
                   
                 ACTATTAGCCCGGGGGATCCAGACATGATAAGATACATTGATGAG 
               
               
                   
                 TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT 
               
               
                   
                 TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC 
               
               
                   
                 TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT 
               
               
                   
                 CAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAAC 
               
               
                   
                 CTCTACAAATGTGGTATGGCTGATTATGATCCGGCTGCCTCGCGC 
               
               
                   
                 GTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGG 
               
               
                   
                 AGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAG 
               
               
                   
                 CCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAG 
               
               
                   
                 CCATGAGGTCGATCGACTCTAGAGGATCGATCCCCGCCCCGGACG 
               
               
                   
                 AACTAAACCTGACTACGACATCTCTGCCCCTTCTTCGCGGGGCAG 
               
               
                   
                 TGCATGTAATCCCTTCAGTTGGTTGGTACAACTTGCCAACTGGGC 
               
               
                   
                 CCTGTTCCACATGTGACACGGGGGGGGACCAAACACAAAGGGGTT 
               
               
                   
                 CTCTGACTGTAGTTGACATCCTTATAAATGGATGTGCACATTTGC 
               
               
                   
                 CAACACTGAGTGGCTTTCATCCTGGAGCAGACTTTGCAGTCTGTG 
               
               
                   
                 GACTGCAACACAACATTGCCTTTATGTGTAACTCTTGGCTGAAGC 
               
               
                   
                 TCTTACACCAATGCTGGGGGACATGTACCTCCCAGGGGCCCAGGA 
               
               
                   
                 AGACTACGGGAGGCTACACCAACGTCAATCAGAGGGGCCTGTGTA 
               
               
                   
                 GCTACCGATAAGCGGACCCTCAAGAGGGCATTAGCAATAGTGTTT 
               
               
                   
                 ATAAGGCCCCCTTGTTAACCCTAAACGGGTAGCATATGCTTCCCG 
               
               
                   
                 GGTAGTAGTATATACTATCCAGACTAACCCTAATTCAATAGCATA 
               
               
                   
                 TGTTACCCAACGGGAAGCATATGCTATCGAATTAGGGTTAGTAAA 
               
               
                   
                 AGGGTCCTAAGGAACAGCGATATCTCCCACCCCATGAGCTGTCAC 
               
               
                   
                 GGTTTTATTTACATGGGGTCAGGATTCCACGAGGGTAGTGAACCA 
               
               
                   
                 TTTTAGTCACAAGGGCAGTGGCTGAAGATCAAGGAGCGGGCAGTG 
               
               
                   
                 AACTCTCCTGAATCTTCGCCTGCTTCTTCATTCTCCTTCGTTTAG 
               
               
                   
                 CTAATAGAATAACTGCTGAGTTGTGAACAGTAAGGTGTATGTGAG 
               
               
                   
                 GTGCTCGAAAACAAGGTTTCAGGTGACGCCCCCAGAATAAAATTT 
               
               
                   
                 GGACGGGGGGTTCAGTGGTGGCATTGTGCTATGACACCAATATAA 
               
               
                   
                 CCCTCACAAACCCCTTGGGCAATAAATACTAGTGTAGGAATGAAA 
               
               
                   
                 CATTCTGAATATCTTTAACAATAGAAATCCATGGGGTGGGGACAA 
               
               
                   
                 GCCGTAAAGACTGGATGTCCATCTCACACGAATTTATGGCTATGG 
               
               
                   
                 GCAACACATAATCCTAGTGCAATATGATACTGGGGTTATTAAGAT 
               
               
                   
                 GTGTCCCAGGCAGGGACCAAGACAGGTGAACCATGTTGTTACACT 
               
               
                   
                 CTATTTGTAACAAGGGGAAAGAGAGTGGACGCCGACAGCAGCGGA 
               
               
                   
                 CTCCACTGGTTGTCTCTAACACCCCCGAAAATTAAACGGGGCTCC 
               
               
                   
                 ACGCCAATGGGGCCCATAAACAAAGACAAGTGGCCACTCTTTTTT 
               
               
                   
                 TTGAAATTGTGGAGTGGGGGCACGCGTCAGCCCCCACACGCCGCC 
               
               
                   
                 CTGCGGTTTTGGACTGTAAAATAAGGGTGTAATAACTTGGCTGAT 
               
               
                   
                 TGTAACCCCGCTAACCACTGCGGTCAAACCACTTGCCCACAAAAC 
               
               
                   
                 CACTAATGGCACCCCGGGGAATACCTGCATAAGTAGGTGGGCGGG 
               
               
                   
                 CCAAGATAGGGGCGCGATTGCTGCGATCTGGAGGACAAATTACAC 
               
               
                   
                 ACACTTGCGCCTGAGCGCCAAGCACAGGGTTGTTGGTCCTCATAT 
               
               
                   
                 TCACGAGGTCGCTGAGAGCACGGTGGGCTAATGTTGCCATGGGTA 
               
               
                   
                 GCATATACTACCCAAATATCTGGATAGCATATGCTATCCTAATCT 
               
               
                   
                 ATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCAT 
               
               
                   
                 ATGCTATCCTAATCTATATCTGGGTAGTATATGCTATCCTAATTT 
               
               
                   
                 ATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCAT 
               
               
                   
                 ATGCTATCCTAATCTATATCTGGGTAGTATATGCTATCCTAATCT 
               
               
                   
                 GTATCCGGGTAGCATATGCTATCCTAATAGAGATTAGGGTAGTAT 
               
               
                   
                 ATGCTATCCTAATTTATATCTGGGTAGCATATACTACCCAAATAT 
               
               
                   
                 CTGGATAGCATATGCTATCCTAATCTATATCTGGGTAGCATATGC 
               
               
                   
                 TATCCTAATCTATATCTGGGTAGCATAGGCTATCCTAATCTATAT 
               
               
                   
                 CTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGC 
               
               
                   
                 TATCCTAATTTATATCTGGGTAGCATAGGCTATCCTAATCTATAT 
               
               
                   
                 CTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGC 
               
               
                   
                 TATCCTAATCTGTATCCGGGTAGCATATGCTATCCTCATGCATAT 
               
               
                   
                 ACAGTCAGCATATGATACCCAGTAGTAGAGTGGGAGTGCTATCCT 
               
               
                   
                 TTGCATATGCCGCCACCTCCCAAGGGGGCGTGAATTTTCGCTGCT 
               
               
                   
                 TGTCCTTTTCCTGCATGCTGGTTGCTCCCATTCTTAGGTGAATTT 
               
               
                   
                 AAGGAGGCCAGGCTAAAGCCGTCGCATGTCTGATTGCTCACCAGG 
               
               
                   
                 TAAATGTCGCTAATGTTTTCCAACGCGAGAAGGTGTTGAGCGCGG 
               
               
                   
                 AGCTGAGTGACGTGACAACATGGGTATGCCCAATTGCCCCATGTT 
               
               
                   
                 GGGAGGACGAAAATGGTGACAAGACAGATGGCCAGAAATACACCA 
               
               
                   
                 ACAGCACGCATGATGTCTACTGGGGATTTATTCTTTAGTGCGGGG 
               
               
                   
                 GAATACACGGCTTTTAATACGATTGAGGGCGTCTCCTAACAAGTT 
               
               
                   
                 ACATCACTCCTGCCCTTCCTCACCCTCATCTCCATCACCTCCTTC 
               
               
                   
                 ATCTCCGTCATCTCCGTCATCACCCTCCGCGGCAGCCCCTTCCAC 
               
               
                   
                 CATAGGTGGAAACCAGGGAGGCAAATCTACTCCATCGTCAAAGCT 
               
               
                   
                 GCACACAGTCACCCTGATATTGCAGGTAGGAGCGGGCTTTGTCAT 
               
               
                   
                 AACAAGGTCCTTAATCGCATCCTTCAAAACCTCAGCAAATATATG 
               
               
                   
                 AGTTTGTAAAAAGACCATGAAATAACAGACAATGGACTCCCTTAG 
               
               
                   
                 CGGGCCAGGTTGTGGGCCGGGTCCAGGGGCCATTCCAAAGGGGAG 
               
               
                   
                 ACGACTCAATGGTGTAAGACGACATTGTGGAATAGCAAGGGCAGT 
               
               
                   
                 TCCTCGCCTTAGGTTGTAAAGGGAGGTCTTACTACCTCCATATAC 
               
               
                   
                 GAACACACCGGCGACCCAAGTTCCTTCGTCGGTAGTCCTTTCTAC 
               
               
                   
                 GTGACTCCTAGCCAGGAGAGCTCTTAAACCTTCTGCAATGTTCTC 
               
               
                   
                 AAATTTCGGGTTGGAACCTCCTTGACCACGATGCTTTCCAAACCA 
               
               
                   
                 CCCTCCTTTTTTGCGCCTGCCTCCATCACCCTGACCCCGGGGTCC 
               
               
                   
                 AGTGCTTGGGCCTTCTCCTGGGTCATCTGCGGGGCCCTGCTCTAT 
               
               
                   
                 CGCTCCCGGGGGCACGTCAGGCTCACCATCTGGGCCACCTTCTTG 
               
               
                   
                 GTGGTATTCAAAATAATCGGCTTCCCCTACAGGGTGGAAAAATGG 
               
               
                   
                 CCTTCTACCTGGAGGGGGCCTGCGCGGTGGAGACCCGGATGATGA 
               
               
                   
                 TGACTGACTACTGGGACTCCTGGGCCTCTTTTCTCCACGTCCACG 
               
               
                   
                 ACCTCTCCCCCTGGCTCTTTCACGACTTCCCCCCCTGGCTCTTTC 
               
               
                   
                 ACGTCCTCTACCCCGGCGGCCTCCACTACCTCCTCGACCCCGGCC 
               
               
                   
                 TCCACTACCTCCTCGACCCCGGCCTCCACTGCCTCCTCGACCCCG 
               
               
                   
                 GCCTCCACCTCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCCTCC 
               
               
                   
                 TGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGC 
               
               
                   
                 CCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCCTGC 
               
               
                   
                 CCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCTCCTGCCCC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGC 
               
               
                   
                 CCCTCCTCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCC 
               
               
                   
                 TCCTGCCCCTCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCC 
               
               
                   
                 TGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCC 
               
               
                   
                 TGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCCCCTCC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCC 
               
               
                   
                 TGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTGCTCCTGCCCCTCCCGCTCCTGCTCCTGCTCCTGT 
               
               
                   
                 TCCACCGTGGGTCCCTTTGCAGCCAATGCAACTTGGACGTTTTTG 
               
               
                   
                 GGGTCTCCGGACACCATCTCTATGTCTTGGCCCTGATCCTGAGCC 
               
               
                   
                 GCCCGGGGCTCCTGGTCTTCCGCCTCCTCGTCCTCGTCCTCTTCC 
               
               
                   
                 CCGTCCTCGTCCATGGTTATCACCCCCTCTTCTTTGAGGTCCACT 
               
               
                   
                 GCCGCCGGAGCCTTCTGGTCCAGATGTGTCTCCCTTCTCTCCTAG 
               
               
                   
                 GCCATTTCCAGGTCCTGTACCTGGCCCCTCGTCAGACATGATTCA 
               
               
                   
                 CACTAAAAGAGATCAATAGACATCTTTATTAGACGACGCTCAGTG 
               
               
                   
                 AATACAGGGAGTGCAGACTCCTGCCCCCTCCAACAGCCCCCCCAC 
               
               
                   
                 CCTCATCCCCTTCATGGTCGCTGTCAGACAGATCCAGGTCTGAAA 
               
               
                   
                 ATTCCCCATCCTCCGAACCATCCTCGTCCTCATCACCAATTACTC 
               
               
                   
                 GCAGCCCGGAAAACTCCCGCTGAACATCCTCAAGATTTGCGTCCT 
               
               
                   
                 GAGCCTCAAGCCAGGCCTCAAATTCCTCGTCCCCCTTTTTGCTGG 
               
               
                   
                 ACGGTAGGGATGGGGATTCTCGGGACCCCTCCTCTTCCTCTTCAA 
               
               
                   
                 GGTCACCAGACAGAGATGCTACTGGGGCAACGGAAGAAAAGCTGG 
               
               
                   
                 GTGCGGCCTGTGAGGATCAGCTTATCGATGATAAGCTGTCAAACA 
               
               
                   
                 TGAGAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTT 
               
               
                   
                 ATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGG 
               
               
                   
                 CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTT 
               
               
                   
                 CTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTG 
               
               
                   
                 ATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCA 
               
               
                   
                 ACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCT 
               
               
                   
                 TCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGC 
               
               
                   
                 TGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCT 
               
               
                   
                 CAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTT 
               
               
                   
                 TCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATT 
               
               
                   
                 ATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA 
               
               
                   
                 CTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAA 
               
               
                   
                 GCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGC 
               
               
                   
                 CATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAAC 
               
               
                   
                 GATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGG 
               
               
                   
                 GGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGA 
               
               
                   
                 AGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAAT 
               
               
                   
                 GGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCT 
               
               
                   
                 AGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGT 
               
               
                   
                 TGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTAT 
               
               
                   
                 TGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCAT 
               
               
                   
                 TGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTAT 
               
               
                   
                 CTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACA 
               
               
                   
                 GATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTC 
               
               
                   
                 AGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCA 
               
               
                   
                 TTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCT 
               
               
                   
                 CATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTC 
               
               
                   
                 AGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACC 
               
               
                   
                 AGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCC 
               
               
                   
                 GAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCT 
               
               
                   
                 TCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGC 
               
               
                   
                 ACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGC 
               
               
                   
                 TGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACG 
               
               
                   
                 ATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTC 
               
               
                   
                 GTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAG 
               
               
                   
                 ATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGG 
               
               
                   
                 GAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGG 
               
               
                   
                 AGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTA 
               
               
                   
                 TAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT 
               
               
                   
                 GTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA 
               
               
                   
                 CGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTAT 
               
               
                   
                 TACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGAC 
               
               
                   
                 CGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGAT 
               
               
                   
                 GCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAT 
               
               
                   
                 ATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAG 
               
               
                   
                 CCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGG 
               
               
                   
                 CTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTC 
               
               
                   
                 AGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAG 
               
               
                   
                 TATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCC 
               
               
                   
                 CCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCA 
               
               
                   
                 TTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGC 
               
               
                   
                 CGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGG 
               
               
                   
                 CTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTGCATGCCTGCA 
               
               
                   
                 GGTCGGCCGCCACGACCGGTGCCGCCACCATCCCCTGACCCACGC 
               
               
                   
                 CCCTGACCCCTCACAAGGAGACGACCTTCCATGACCGAGTACAAG 
               
               
                   
                 CCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCCGGGCCGTA 
               
               
                   
                 CGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCAC 
               
               
                   
                 ACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAA 
               
               
                   
                 GAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGG 
               
               
                   
                 GTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAG 
               
               
                   
                 AGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATG 
               
               
                   
                 GCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAA 
               
               
                   
                 GGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTG 
               
               
                   
                 GCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGC 
               
               
                   
                 AGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGG 
               
               
                   
                 GTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTC 
               
               
                   
                 TACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCC 
               
               
                   
                 GAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGA 
               
               
                   
                 CGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAGGAGCGCACGA 
               
               
                   
                 CCCCATGGCTCCGACCGAAGCCGACCCGGGCGGCCCCGCCGACCC 
               
               
                   
                 CGCACCCGCCCCCGAGGCCCACCGACTCTAGAGGATCATAATCAG 
               
               
                   
                 CCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCC 
               
               
                   
                 ACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGT 
               
               
                   
                 TGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAA 
               
               
                   
                 TAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTC 
               
               
                   
                 TAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTG 
               
               
                   
                 GATCACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGA 
               
               
                   
                 GGGCAAAGGAATAGGGGAGATGGGGGAGGCTAACTGAAACACGGA 
               
               
                   
                 AGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAA 
               
               
                   
                 GACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGC 
               
               
                   
                 GGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGA 
               
               
                   
                 CCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCC 
               
               
                   
                 CACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTC 
               
               
                   
                 GGGGCGGCAGGCCCTGCCATAGCCACTGGCCCCGTGGGTTAGGGA 
               
               
                   
                 CGGGGTCCCCCATGGGGAATGGTTTATGGTTCGTGGGGGTTATTA 
               
               
                   
                 TTTTGGGCGTTGCGTGGGGTCTGGTCCACGACTGGACTGAGCAGA 
               
               
                   
                 CAGACCCATGGTTTTTGGATGGCCTGGGCATGGACCGCATGTACT 
               
               
                   
                 GGCGCGACACGAACACCGGGCGTCTGTGGCTGCCAAACACCCCCG 
               
               
                   
                 ACCCCCAAAAACCACCGCGCGGATTTCTGGCGTGCCAAGCTAGTC 
               
               
                   
                 GACCAATTCTCATGTTTGACAGCTTATCATCGCAGATCCGGGCAA 
               
               
                   
                 CGTTGTTGCATTGCTGCAGGCGCAGAACTGGTAGGTATGGAAGAT 
               
               
                   
                 CTCTAGAAGCTGGGTACCAGCTGCTAGCAAGCTTGCTAGCGGCCG 
               
               
                   
                 GCTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTT 
               
               
                   
                 TACTCCCTATCAGTGATAGAGAACGATGTCGAGTTTACTCCCTAT 
               
               
                   
                 CAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAG 
               
               
                   
                 AGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTAT 
               
               
                   
                 GTCGAGTTTATCCCTATCAGTGATAGAGAACGTATGTCGAGTTTA 
               
               
                   
                 CTCCCTATCAGTGATAGAGAACGTATGTCGAGGTAGGCGTGTACG 
               
               
                   
                 GTGGGAGGCCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAT 
               
               
                   
                 CGCCG 
               
               
                   
                 (SEQ ID NO: 40) 
               
               
                   
                   
               
               
                   
                 LINE-1 plasmid Her2-Cd3z-T2A GFP intron 
               
               
                   
                 (SEQ ID NO: 41) 
               
               
                   
                 CGGCCGCGGGGGGAGGAGCCAAGATGGCCGAATAGGAACAGCTCC 
               
               
                   
                 GGTCTACAGCTCCCAGCGTGAGCGACGCAGAAGACGGTGATTTCT 
               
               
                   
                 GCATTTCCATCTGAGGTACCGGGTTCATCTCACTAGGGAGTGCCA 
               
               
                   
                 GACAGTGGGCGCAGGCCAGTGTGTGTGCGCACCGTGCGCGAGCCG 
               
               
                   
                 AAGCAGGGCGAGGCATTGCCTCACCTGGGAAGCGCAAGGGGTCAG 
               
               
                   
                 GGAGTTCCCTTTCCGAGTCAAAGAAAGGGGTGACGGACGCACCTG 
               
               
                   
                 GAAAATCGGGTCACTCCCACCCGAATATTGCGCTTTTCAGACCGG 
               
               
                   
                 CTTAAGAAACGGCGCACCACGAGACTATATCCCACACCTGGCTCG 
               
               
                   
                 GAGGGTCCTACGCCCACGGAATCTCGCTGATTGCTAGCACAGCAG 
               
               
                   
                 TCTGAGATCAAACTGCAAGGCGGCAACGAGGCTGGGGGAGGGGCG 
               
               
                   
                 CCCGCCATTGCCCAGGCTTGCTTAGGTAAACAAAGCAGCAGGGAA 
               
               
                   
                 GCTCGAACTGGGTGGAGCCCACCACAGCTCAAGGAGGCCTGCCTG 
               
               
                   
                 CCTCTGTAGGCTCCACCTCTGGGGGCAGGGCACAGACAAACAAAA 
               
               
                   
                 AGACAGCAGTAACCTCTGCAGACTTAAGTGTCCCTGTCTGACAGC 
               
               
                   
                 TTTGAAGAGAGCAGTGGTTCTCCCAGCACGCAGCTGGAGATCTGA 
               
               
                   
                 GAACGGGCAGACTGCCTCCTCAAGTGGGTCCCTGACCCCTGACCC 
               
               
                   
                 CCGAGCAGCCTAACTGGGAGGCACCCCCCAGCAGGGGCACACTGA 
               
               
                   
                 CACCTCACACGGCAGGGTATTCCAACAGACCTGCAGCTGAGGGTC 
               
               
                   
                 CTGTCTGTTAGAAGGAAAACTAACAACCAGAAAGGACATCTACAC 
               
               
                   
                 CGAAAACCCATCTGTACATCACCATCATCAAAGACCAAAAGTAGA 
               
               
                   
                 TAAAACCACAAAGATGGGGAAAAAACAGAACAGAAAAACTGGAAA 
               
               
                   
                 CTCTAAAACGCAGAGCGCCTCTCCTCCTCCAAAGGAACGCAGTTC 
               
               
                   
                 CTCACCAGCAACAGAACAAAGCTGGATGGAGAATGATTTTGATGA 
               
               
                   
                 GCTGAGAGAAGAAGGCTTCAGACGATCAAATTACTCTGAGCTACG 
               
               
                   
                 GGAGGACATTCAAACCAAAGGCAAAGAAGTTGAAAACTTTGAAAA 
               
               
                   
                 AAATTTAGAAGAATGTATAACTAGAATAACCAATACAGAGAAGTG 
               
               
                   
                 CTTAAAGGAGCTGATGGAGCTGAAAACCAAGGCTCGAGAACTACG 
               
               
                   
                 TGAAGAATGCAGAAGCCTCAGGAGCCGATGCGATCAACTGGAAGA 
               
               
                   
                 AAGGGTATCAGCAATGGAAGATGAAATGAATGAAATGAAGCGAGA 
               
               
                   
                 AGGGAAGTTTAGAGAAAAAAGAATAAAAAGAAATGAGCAAAGCCT 
               
               
                   
                 CCAAGAAATATGGGACTATGTGAAAAGACCAAATCTACGTCTGAT 
               
               
                   
                 TGGTGTACCTGAAAGTGATGTGGAGAATGGAACCAAGTTGGAAAA 
               
               
                   
                 CACTCTGCAGGATATTATCCAGGAGAACTTCCCCAATCTAGCAAG 
               
               
                   
                 GCAGGCCAACGTTCAGATTCAGGAAATACAGAGAACGCCACAAAG 
               
               
                   
                 ATACTCCTCGAGAAGAGCAACTCCAAGACACATAATTGTCAGATT 
               
               
                   
                 CACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGA 
               
               
                   
                 GAAAGGTCGGGTTACCCTCAAAGGAAAGCCCATCAGACTAACAGC 
               
               
                   
                 GGATCTCTCGGCAGAAACCCTACAAGCCAGAAGAGAGTGGGGGCC 
               
               
                   
                 AATATTCAACATTCTTAAAGAAAAGAATTTTCAACCCAGAATTTC 
               
               
                   
                 ATATCCAGCCAAACTAAGCTTCATAAGTGAAGGAGAAATAAAATA 
               
               
                   
                 CTTTATAGACAAGCAAATGTTGAGAGATTTTGTCACCACCAGGCC 
               
               
                   
                 TGCCCTAAAAGAGCTCCTGAAGGAAGCGCTAAACATGGAAAGGAA 
               
               
                   
                 CAACCGGTACCAGCCGCTGCAAAATCATGCCAAAATGTAAAGACC 
               
               
                   
                 ATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACC 
               
               
                   
                 AGCTAACATCATAATGACAGGATCAACTTCACACATAACAATATT 
               
               
                   
                 AACTTTAAATATAAATGGACTAAATTCTGCAATTAAAAGACACAG 
               
               
                   
                 ACTGGCAAGTTGGATAAAGAGTCAAGACCCATCAGTGTGCTGTAT 
               
               
                   
                 TCAGGAAACCCATCTCACGTGCAGAGACACACATAGGCTCAAAAT 
               
               
                   
                 AAAAGGATGGAGGAAGATCTACCAAGCCAATGGAAAACAAAAAAA 
               
               
                   
                 GGCAGGGGTTGCAATCCTAGTCTCTGATAAAACAGACTTTAAACC 
               
               
                   
                 AACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGTAAA 
               
               
                   
                 GGGATCAATTCAACAAGAGGAGCTAACTATCCTAAATATTTATGC 
               
               
                   
                 ACCCAATACAGGAGCACCCAGATTCATAAAGCAAGTCCTCAGTGA 
               
               
                   
                 CCTACAAAGAGACTTAGACTCCCACACATTAATAATGGGAGACTT 
               
               
                   
                 TAACACCCCACTGTCAACATTAGACAGATCAACGAGACAGAAAGT 
               
               
                   
                 CAACAAGGATACCCAGGAATTGAACTCAGCTCTGCACCAAGCAGA 
               
               
                   
                 CCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAATA 
               
               
                   
                 TACATTTTTTTCAGCACCACACCACACCTATTCCAAAATTGACCA 
               
               
                   
                 CATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAAAGAACAGA 
               
               
                   
                 AATTATAACAAACTATCTCTCAGACCACAGTGCAATCAAACTAGA 
               
               
                   
                 ACTCAGGATTAAGAATCTCACTCAAAGCCGCTCAACTACATGGAA 
               
               
                   
                 ACTGAACAACCTGCTCCTGAATGACTACTGGGTACATAACGAAAT 
               
               
                   
                 GAAGGCAGAAATAAAGATGTTCTTTGAAACCAACGAGAACAAAGA 
               
               
                   
                 CACCACATACCAGAATCTCTGGGACGCATTCAAAGCAGTGTGTAG 
               
               
                   
                 AGGGAAATTTATAGCACTAAATGCCTACAAGAGAAAGCAGGAAAG 
               
               
                   
                 ATCCAAAATTGACACCCTAACATCACAATTAAAAGAACTAGAAAA 
               
               
                   
                 GCAAGAGCAAACACATTCAAAAGCTAGCAGAAGGCAAGAAATAAC 
               
               
                   
                 TAAAATCAGAGCAGAACTGAAGGAAATAGAGACACAAAAAACCCT 
               
               
                   
                 TCAAAAAATCAATGAATCCAGGAGCTGGTTTTTTGAAAGGATCAA 
               
               
                   
                 CAAAATTGATAGACCGCTAGCAAGACTAATAAAGAAAAAAAGAGA 
               
               
                   
                 GAAGAATCAAATAGACACAATAAAAAATGATAAAGGGGATATCAC 
               
               
                   
                 CACCGATCCCACAGAAATACAAACTACCATCAGAGAATACTACAA 
               
               
                   
                 ACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAC 
               
               
                   
                 ATTCCTCGACACATACACTCTCCCAAGACTAAACCAGGAAGAAGT 
               
               
                   
                 TGAATCTCTGAATCGACCAATAACAGGCTCTGAAATTGTGGCAAT 
               
               
                   
                 AATCAATAGTTTACCAACCAAAAAGAGTCCAGGACCAGATGGATT 
               
               
                   
                 CACAGCCGAATTCTACCAGAGGTACAAGGAGGAACTGGTACCATT 
               
               
                   
                 CCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCC 
               
               
                   
                 TAACTCATTTTATGAGGCCAGCATCATTCTGATACCAAAGCCGGG 
               
               
                   
                 CAGAGACACAACCAAAAAAGAGAATTTTAGACCAATATCCTTGAT 
               
               
                   
                 GAACATTGATGCAAAAATCCTCAATAAAATACTGGCAAACCGAAT 
               
               
                   
                 CCAGCAGCACATCAAAAAGCTTATCCACCATGATCAAGTGGGCTT 
               
               
                   
                 CATCCCTGGGATGCAAGGCTGGTTCAATATACGCAAATCAATAAA 
               
               
                   
                 TGTAATCCAGCATATAAACAGAGCCAAAGACAAAAACCACATGAT 
               
               
                   
                 TATCTCAATAGATGCAGAAAAAGCCTTTGACAAAATTCAACAACC 
               
               
                   
                 CTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACGTA 
               
               
                   
                 TTTCAAAATAATAAGAGCTATCTATGACAAACCCACAGCCAATAT 
               
               
                   
                 CATACTGAATGGGCAAAAACTGGAAGCATTCCCTTTGAAAACCGG 
               
               
                   
                 CACAAGACAGGGATGCCCTCTCTCACCGCTCCTATTCAACATAGT 
               
               
                   
                 GTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGGAAATAAA 
               
               
                   
                 GGGTATTCAATTAGGAAAAGAGGAAGTCAAATTGTCCCTGTTTGC 
               
               
                   
                 AGACGACATGATTGTTTATCTAGAAAACCCCATCGTCTCAGCCCA 
               
               
                   
                 AAATCTCCTTAAGCTGATAAGCAACTTCAGCAAAGTCTCAGGATA 
               
               
                   
                 CAAAATCAATGTACAAAAATCACAAGCATTCTTATACACCAACAA 
               
               
                   
                 CAGACAAACAGAGAGCCAAATCATGGGTGAACTCCCATTCACAAT 
               
               
                   
                 TGCTTCAAAGAGAATAAAATACCTAGGAATCCAACTTACAAGGGA 
               
               
                   
                 TGTGAAGGACCTCTTCAAGGAGAACTACAAACCACTGCTCAAGGA 
               
               
                   
                 AATAAAAGAGGAGACAAACAAATGGAAGAACATTCCATGCTCATG 
               
               
                   
                 GGTAGGAAGAATCAATATCGTGAAAATGGCCATACTGCCCAAGGT 
               
               
                   
                 AATTTACAGATTCAATGCCATCCCCATCAAGCTACCAATGACTTT 
               
               
                   
                 CTTCACAGAATTGGAAAAAACTACTTTAAAGTTCATATGGAACCA 
               
               
                   
                 AAAAAGAGCCCGCATTGCCAAGTCAATCCTAAGCCAAAAGAACAA 
               
               
                   
                 AGCTGGAGGCATCACACTACCTGACTTCAAACTATACTACAAGGC 
               
               
                   
                 TACAGTAACCAAAACAGCATGGTACTGGTACCAAAACAGAGATAT 
               
               
                   
                 AGATCAATGGAACAGAACAGAGCCCTCAGAAATAATGCCGCATAT 
               
               
                   
                 CTACAACTATCTGATCTTTGACAAACCTGAGAAAAACAAGCAATG 
               
               
                   
                 GGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCT 
               
               
                   
                 AGCCATATGTAGAAAGCTGAAACTGGATCCCTTCCTTACACCTTA 
               
               
                   
                 TACAAAAATCAATTCAAGATGGATTAAAGATTTAAACGTTAAACC 
               
               
                   
                 TAAAACCATAAAAACCCTAGAAGAAAACCTAGGCATTACCATTCA 
               
               
                   
                 GGACATAGGCGTGGGCAAGGACTTCATGTCCAAAACACCAAAAGC 
               
               
                   
                 AATGGCAACAAAAGACAAAATTGACAAATGGGATCTAATTAAACT 
               
               
                   
                 AAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAG 
               
               
                   
                 GCAACCTACAACATGGGAGAAAATTTTTGCAACCTACTCATCTGA 
               
               
                   
                 CAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTA 
               
               
                   
                 CAAGAAAAAAACAAACAACCCCATCAAAAAGTGGGCGAAGGACAT 
               
               
                   
                 GAACAGACACTTCTCAAAAGAAGACATTTATGCAGCCAAAAAACA 
               
               
                   
                 CATGAAGAAATGCTCATCATCACTGGCCATCAGAGAAATGCAAAT 
               
               
                   
                 CAAAACCACTATGAGATATCATCTCACACCAGTTAGAATGGCAAT 
               
               
                   
                 CATTAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGCGGAGA 
               
               
                   
                 AATAGGAACACTTTTACACTGTTGGTGGGACTGTAAACTAGTTCA 
               
               
                   
                 ACCATTGTGGAAGTCAGTGTGGCGATTCCTCAGGGATCTAGAACT 
               
               
                   
                 AGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCC 
               
               
                   
                 AAATGAGTATAAATCATGCTGCTATAAAGACACATGCACACGTAT 
               
               
                   
                 GTTTATTGCGGCACTATTCACAATAGCAAAGACTTGGAACCAACC 
               
               
                   
                 CAAATGTCCAACAATGATAGACTGGATTAAGAAAATGTGGCACAT 
               
               
                   
                 ATACACCATGGAATACTATGCAGCCATAAAAAATGATGAGTTCAT 
               
               
                   
                 ATCCTTTGTAGGGACATGGATGAAATTGGAAACCATCATTCTCAG 
               
               
                   
                 TAAACTATCGCAAGAACAAAAAACCAAACACCGCATATTCTCACT 
               
               
                   
                 CATAGGTGGGAATTGAACAATGAGATCACATGGACACAGGAAGGG 
               
               
                   
                 GAATATCACACTCTGGGGACTGTGGTGGGGTCGGGGGAGGGGGGA 
               
               
                   
                 GGGATAGCATTGGGAGATATACCTAATGCTAGATGACACATTAGT 
               
               
                   
                 GGGTGCAGCGCACCAGCATGGCACATGTATACGGATCCGAATTCT 
               
               
                   
                 CGACGGATCGATCCGAACAAACGACCCAACACCCGTGCGTTTTAT 
               
               
                   
                 TCTGTCTTTTTATTGCCGATCCCCTCAGAAGAACTCGTCAAGAAG 
               
               
                   
                 GCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTA 
               
               
                   
                 AAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGC 
               
               
                   
                 AATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCGGCCGC 
               
               
                   
                 TTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGG 
               
               
                   
                 TCACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGT 
               
               
                   
                 CTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCA 
               
               
                   
                 GCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGT 
               
               
                   
                 CGGCCAGGTGAGTCCAGGAGATGTTTCAGCACTGTTGCCTTTAGT 
               
               
                   
                 CTCGAGGCAACTTAGACAACTGAGTATTGATCTGAGCACAGCAGG 
               
               
                   
                 GTGTGAGCTGTTTGAAGATACTGGGGTTGGGGGTGAAGAAACTGC 
               
               
                   
                 AGAGGACTAACTGGGCTGAGACCCAGTGGCAATGTTTTAGGGCCT 
               
               
                   
                 AAGGAATGCCTCTGAAAATCTAGATGGACAACTTTGACTTTGAGA 
               
               
                   
                 AAAGAGAGGTGGAAATGAGGAAAATGACTTTTCTTTATTAGATTT 
               
               
                   
                 CGGTAGAAAGAACTTTCATCTTTCCCCTATTTTTGTTATTCGTTT 
               
               
                   
                 TAAAACATCTATCTGGAGGCAGGACAAGTATGGTCATTAAAAAGA 
               
               
                   
                 TGCAGGCAGAAGGCATATATTGGCTCAGTCAAAGTGGGGAACTTT 
               
               
                   
                 GGTGGCCAAACATACATTGCTAAGGCTATTCCTATATCAGCTGGA 
               
               
                   
                 CACATATAAAATGCTGCTAATGCTTCATTACAAACTTATATCCTT 
               
               
                   
                 TAATTCCAGATGGGGGCAAAGTATGTCCAGGGGTGAGGAACAATT 
               
               
                   
                 GAAACATTTGGGCTGGAGTAGATTTTGAAAGTCAGCTCTGTGTGT 
               
               
                   
                 GTGTGTGTGTGTGTGTGTGTGAGAGCGTGTGTTTCTTTTAACGTT 
               
               
                   
                 TTCAGCCTACAGCATACAGGGTTCATGGTGGCAAGAAGATAACAA 
               
               
                   
                 GATTTAAATTATGGCCAGTGACTAGTGCTGCAAGAAGAACAACTA 
               
               
                   
                 CCTGCATTTAATGGGAAAGCAAAATCTCAGGCTTTGAGGGAAGTT 
               
               
                   
                 AACATAGGCTTGATTCTGGGTGGAAGCTGGGTGTGTAGTTATCTG 
               
               
                   
                 GAGGCCAGGCTGGAGCTCTCAGCTCACTATGGGTTCATCTTTATT 
               
               
                   
                 GTCTCCTTTCATCTCAACAGCTGCACGCTGCCGTCCTCGATGTTG 
               
               
                   
                 TGGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCG 
               
               
                   
                 GCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTG 
               
               
                   
                 TGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGC 
               
               
                   
                 TCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCG 
               
               
                   
                 CGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCC 
               
               
                   
                 TGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAATAGTGAACCG 
               
               
                   
                 TCAGATCGCCGGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTG 
               
               
                   
                 AAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAG 
               
               
                   
                 GGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGC 
               
               
                   
                 TTGCCGTAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAAC 
               
               
                   
                 TTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACC 
               
               
                   
                 ACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATAGGGCCGGGA 
               
               
                   
                 TTCTCCTCCACGTCACCGCATGTTAGAAGACTTCCTCTGCCCTCT 
               
               
                   
                 CTTGGAGGCAGGGCCTGCATGTGCAGGGCATCGTAGGTATCCTTG 
               
               
                   
                 GTGGCTGTGCTCAGTCCCTGGTACAGTCCATCGTGGCCCTTGCCT 
               
               
                   
                 CTTCTTCTCTCGCCCTTCATGCCGATCTCGCTGTAGGCCTCGGCC 
               
               
                   
                 ATCTTGTCTTTCTGCAGCTCATTATACAGGCCCTCTTGAGGATTC 
               
               
                   
                 TTTCTCCGCTGGGGCTTGCCGCCCATCTCAGGATCTCTGCCTCTC 
               
               
                   
                 CGCTTATCCAGCACGTCGTACTCTTCTCTTCTCCCCAGGTTCAGC 
               
               
                   
                 TCGTTGTACAGCTGATTCTGGCCCTGCTGGTAAGCAGGAGCGTCG 
               
               
                   
                 GCGGATCTGCTGAACTTCACTCTGCAGTACAGGGTGATGACCAGA 
               
               
                   
                 GAGAGCAGCAGAACGCCACATGTGCCAGCCAGAGGGGCCCAAATG 
               
               
                   
                 TAGATATCCAGGCCTCTGGTATGCACAGCTCCGCCAGCTGCAGGT 
               
               
                   
                 CTACAGGCTTCAGGTCTGAGAGACAGAGGCTGGCTGGCGATTGTA 
               
               
                   
                 GGAGCTGGTGTAGGTGGTCTAGGAGCGGGTGTTGTTGTAGGCTTG 
               
               
                   
                 GCGGGCAGAAACACGGGCACGAAGTGGCTGAAGTACATGATGCTA 
               
               
                   
                 TTGCTCAGGGCTCCGCTTCCTCCGCCTCCGCTAGAAGAAACTGTG 
               
               
                   
                 ACCAGGGTGCCCTGTCCCCAAACATCCATGGCGTAGAAGCCGTCG 
               
               
                   
                 CCTCCCCATCTAGAACAGTAGTACACGGCGGTGTCCTCGGCTCTC 
               
               
                   
                 AGGCTGTTCATCTGCAGGTAGGCGGTGTTCTTGCTGGTGTCGGCG 
               
               
                   
                 CTGATGGTGAATCTGCCCTTCACGCTATCGGCGTATCTGGTGTAG 
               
               
                   
                 CCGTTGGTGGGGTAGATTCTGGCGACCCATTCAAGTCCCTTTCCA 
               
               
                   
                 GGGGCCTGTCGGACCCAGTGGATGTAGGTGTCCTTGATGTTGAAG 
               
               
                   
                 CCGCTGGCGGCACAAGACAGTCTCAGAGAGCCGCCAGGCTGAACA 
               
               
                   
                 AGTCCTCCGCCAGATTCAACCAGCTGCACCTCAGATCCTTCGCCA 
               
               
                   
                 GATCCAGGCTTTCCAGAGCCGCTGGTGCTGCCTGTTCTCTTGATT 
               
               
                   
                 TCCACCTTGGTGCCCTGGCCAAAGGTTGGAGGTGTGGTGTAGTGC 
               
               
                   
                 TGCTGGCAGTAGTAGGTGGCGAAGTCCTCAGGCTGCAGGCTAGAG 
               
               
                   
                 ATGGTCAGGGTGAAGTCGGTGCCAGATCTGCTGCCGCTGAATCTG 
               
               
                   
                 CTTGGCACGCCGCTGTACAGAAAGCTGGCGCTGTAGATCAGCAGC 
               
               
                   
                 TTAGGGGCTTTTCCAGGCTTCTGCTGATACCAGGCCACGGCGGTA 
               
               
                   
                 TTCACATCCTGGCTGGCTCTACAGGTGATGGTCACTCTATCGCCC 
               
               
                   
                 ACAGAGGCAGACAGGCTGCTAGGGCTCTGTGTCATCTGGATGTCG 
               
               
                   
                 CTGATGCTGCAGGCCACTGTTCCCAGCAGCAGCAGAGACTGCAGC 
               
               
                   
                 CACATTCGAAGCTTGAGCTCGAGATCTGAGTCCGGTAGCGCTAGC 
               
               
                   
                 GGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCC 
               
               
                   
                 ACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGT 
               
               
                   
                 TACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAAC 
               
               
                   
                 TCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGT 
               
               
                   
                 CAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCA 
               
               
                   
                 CCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAG 
               
               
                   
                 GAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCC 
               
               
                   
                 ATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGAT 
               
               
                   
                 ACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCA 
               
               
                   
                 CCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAA 
               
               
                   
                 CATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTC 
               
               
                   
                 AGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCA 
               
               
                   
                 TATATGGGCTATGAACTAATGACCCCGTAATTGATTACTATTAGC 
               
               
                   
                 CCGGGGGATCCAGACATGATAAGATACATTGATGAGTTTGGACAA 
               
               
                   
                 ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT 
               
               
                   
                 TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA 
               
               
                   
                 CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG 
               
               
                   
                 GGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAA 
               
               
                   
                 TGTGGTATGGCTGATTATGATCCGGCTGCCTCGCGCGTTTCGGTG 
               
               
                   
                 ATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA 
               
               
                   
                 CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGG 
               
               
                   
                 GCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGAGGT 
               
               
                   
                 CGATCGACTCTAGAGGATCGATCCCCGCCCCGGACGAACTAAACC 
               
               
                   
                 TGACTACGACATCTCTGCCCCTTCTTCGCGGGGCAGTGCATGTAA 
               
               
                   
                 TCCCTTCAGTTGGTTGGTACAACTTGCCAACTGGGCCCTGTTCCA 
               
               
                   
                 CATGTGACACGGGGGGGGACCAAACACAAAGGGGTTCTCTGACTG 
               
               
                   
                 TAGTTGACATCCTTATAAATGGATGTGCACATTTGCCAACACTGA 
               
               
                   
                 GTGGCTTTCATCCTGGAGCAGACTTTGCAGTCTGTGGACTGCAAC 
               
               
                   
                 ACAACATTGCCTTTATGTGTAACTCTTGGCTGAAGCTCTTACACC 
               
               
                   
                 AATGCTGGGGGACATGTACCTCCCAGGGGCCCAGGAAGACTACGG 
               
               
                   
                 GAGGCTACACCAACGTCAATCAGAGGGGCCTGTGTAGCTACCGAT 
               
               
                   
                 AAGCGGACCCTCAAGAGGGCATTAGCAATAGTGTTTATAAGGCCC 
               
               
                   
                 CCTTGTTAACCCTAAACGGGTAGCATATGCTTCCCGGGTAGTAGT 
               
               
                   
                 ATATACTATCCAGACTAACCCTAATTCAATAGCATATGTTACCCA 
               
               
                   
                 ACGGGAAGCATATGCTATCGAATTAGGGTTAGTAAAAGGGTCCTA 
               
               
                   
                 AGGAACAGCGATATCTCCCACCCCATGAGCTGTCACGGTTTTATT 
               
               
                   
                 TACATGGGGTCAGGATTCCACGAGGGTAGTGAACCATTTTAGTCA 
               
               
                   
                 CAAGGGCAGTGGCTGAAGATCAAGGAGCGGGCAGTGAACTCTCCT 
               
               
                   
                 GAATCTTCGCCTGCTTCTTCATTCTCCTTCGTTTAGCTAATAGAA 
               
               
                   
                 TAACTGCTGAGTTGTGAACAGTAAGGTGTATGTGAGGTGCTCGAA 
               
               
                   
                 AACAAGGTTTCAGGTGACGCCCCCAGAATAAAATTTGGACGGGGG 
               
               
                   
                 GTTCAGTGGTGGCATTGTGCTATGACACCAATATAACCCTCACAA 
               
               
                   
                 ACCCCTTGGGCAATAAATACTAGTGTAGGAATGAAACATTCTGAA 
               
               
                   
                 TATCTTTAACAATAGAAATCCATGGGGTGGGGACAAGCCGTAAAG 
               
               
                   
                 ACTGGATGTCCATCTCACACGAATTTATGGCTATGGGCAACACAT 
               
               
                   
                 AATCCTAGTGCAATATGATACTGGGGTTATTAAGATGTGTCCCAG 
               
               
                   
                 GCAGGGACCAAGACAGGTGAACCATGTTGTTACACTCTATTTGTA 
               
               
                   
                 ACAAGGGGAAAGAGAGTGGACGCCGACAGCAGCGGACTCCACTGG 
               
               
                   
                 TTGTCTCTAACACCCCCGAAAATTAAACGGGGCTCCACGCCAATG 
               
               
                   
                 GGGCCCATAAACAAAGACAAGTGGCCACTCTTTTTTTTGAAATTG 
               
               
                   
                 TGGAGTGGGGGCACGCGTCAGCCCCCACACGCCGCCCTGCGGTTT 
               
               
                   
                 TGGACTGTAAAATAAGGGTGTAATAACTTGGCTGATTGTAACCCC 
               
               
                   
                 GCTAACCACTGCGGTCAAACCACTTGCCCACAAAACCACTAATGG 
               
               
                   
                 CACCCCGGGGAATACCTGCATAAGTAGGTGGGCGGGCCAAGATAG 
               
               
                   
                 GGGCGCGATTGCTGCGATCTGGAGGACAAATTACACACACTTGCG 
               
               
                   
                 CCTGAGCGCCAAGCACAGGGTTGTTGGTCCTCATATTCACGAGGT 
               
               
                   
                 CGCTGAGAGCACGGTGGGCTAATGTTGCCATGGGTAGCATATACT 
               
               
                   
                 ACCCAAATATCTGGATAGCATATGCTATCCTAATCTATATCTGGG 
               
               
                   
                 TAGCATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCC 
               
               
                   
                 TAATCTATATCTGGGTAGTATATGCTATCCTAATTTATATCTGGG 
               
               
                   
                 TAGCATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCC 
               
               
                   
                 TAATCTATATCTGGGTAGTATATGCTATCCTAATCTGTATCCGGG 
               
               
                   
                 TAGCATATGCTATCCTAATAGAGATTAGGGTAGTATATGCTATCC 
               
               
                   
                 TAATTTATATCTGGGTAGCATATACTACCCAAATATCTGGATAGC 
               
               
                   
                 ATATGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAAT 
               
               
                   
                 CTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGC 
               
               
                   
                 ATATGCTATCCTAATCTATATCTGGGTAGTATATGCTATCCTAAT 
               
               
                   
                 TTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGC 
               
               
                   
                 ATATGCTATCCTAATCTATATCTGGGTAGTATATGCTATCCTAAT 
               
               
                   
                 CTGTATCCGGGTAGCATATGCTATCCTCATGCATATACAGTCAGC 
               
               
                   
                 ATATGATACCCAGTAGTAGAGTGGGAGTGCTATCCTTTGCATATG 
               
               
                   
                 CCGCCACCTCCCAAGGGGGCGTGAATTTTCGCTGCTTGTCCTTTT 
               
               
                   
                 CCTGCATGCTGGTTGCTCCCATTCTTAGGTGAATTTAAGGAGGCC 
               
               
                   
                 AGGCTAAAGCCGTCGCATGTCTGATTGCTCACCAGGTAAATGTCG 
               
               
                   
                 CTAATGTTTTCCAACGCGAGAAGGTGTTGAGCGCGGAGCTGAGTG 
               
               
                   
                 ACGTGACAACATGGGTATGCCCAATTGCCCCATGTTGGGAGGACG 
               
               
                   
                 AAAATGGTGACAAGACAGATGGCCAGAAATACACCAACAGCACGC 
               
               
                   
                 ATGATGTCTACTGGGGATTTATTCTTTAGTGCGGGGGAATACACG 
               
               
                   
                 GCTTTTAATACGATTGAGGGCGTCTCCTAACAAGTTACATCACTC 
               
               
                   
                 CTGCCCTTCCTCACCCTCATCTCCATCACCTCCTTCATCTCCGTC 
               
               
                   
                 ATCTCCGTCATCACCCTCCGCGGCAGCCCCTTCCACCATAGGTGG 
               
               
                   
                 AAACCAGGGAGGCAAATCTACTCCATCGTCAAAGCTGCACACAGT 
               
               
                   
                 CACCCTGATATTGCAGGTAGGAGCGGGCTTTGTCATAACAAGGTC 
               
               
                   
                 CTTAATCGCATCCTTCAAAACCTCAGCAAATATATGAGTTTGTAA 
               
               
                   
                 AAAGACCATGAAATAACAGACAATGGACTCCCTTAGCGGGCCAGG 
               
               
                   
                 TTGTGGGCCGGGTCCAGGGGCCATTCCAAAGGGGAGACGACTCAA 
               
               
                   
                 TGGTGTAAGACGACATTGTGGAATAGCAAGGGCAGTTCCTCGCCT 
               
               
                   
                 TAGGTTGTAAAGGGAGGTCTTACTACCTCCATATACGAACACACC 
               
               
                   
                 GGCGACCCAAGTTCCTTCGTCGGTAGTCCTTTCTACGTGACTCCT 
               
               
                   
                 AGCCAGGAGAGCTCTTAAACCTTCTGCAATGTTCTCAAATTTCGG 
               
               
                   
                 GTTGGAACCTCCTTGACCACGATGCTTTCCAAACCACCCTCCTTT 
               
               
                   
                 TTTGCGCCTGCCTCCATCACCCTGACCCCGGGGTCCAGTGCTTGG 
               
               
                   
                 GCCTTCTCCTGGGTCATCTGCGGGGCCCTGCTCTATCGCTCCCGG 
               
               
                   
                 GGGCACGTCAGGCTCACCATCTGGGCCACCTTCTTGGTGGTATTC 
               
               
                   
                 AAAATAATCGGCTTCCCCTACAGGGTGGAAAAATGGCCTTCTACC 
               
               
                   
                 TGGAGGGGGCCTGCGCGGTGGAGACCCGGATGATGATGACTGACT 
               
               
                   
                 ACTGGGACTCCTGGGCCTCTTTTCTCCACGTCCACGACCTCTCCC 
               
               
                   
                 CCTGGCTCTTTCACGACTTCCCCCCCTGGCTCTTTCACGTCCTCT 
               
               
                   
                 ACCCCGGCGGCCTCCACTACCTCCTCGACCCCGGCCTCCACTACC 
               
               
                   
                 TCCTCGACCCCGGCCTCCACTGCCTCCTCGACCCCGGCCTCCACC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCCTCCTGCTCCTGC 
               
               
                   
                 CCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTCC 
               
               
                   
                 TGCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGC 
               
               
                   
                 CCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCTCCTGCCCCTCC 
               
               
                   
                 TGCCCCTCCTGCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCCCCTCCTCC 
               
               
                   
                 TGCTCCTGCCCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCCCC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCC 
               
               
                   
                 TGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCCCCTCC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCC 
               
               
                   
                 TCCTGCTCCTGCCCCTCCTGCCCCTCCTGCCCCTCCTCCTGCTCC 
               
               
                   
                 TGCCCCTCCTCCTGCTCCTGCCCCTCCTGCCCCTCCTGCCCCTCC 
               
               
                   
                 TGCCCCTCCTCCTGCTCCTGCCCCTCCTCCTGCTCCTGCCCCTCC 
               
               
                   
                 TGCTCCTGCCCCTCCCGCTCCTGCTCCTGCTCCTGTTCCACCGTG 
               
               
                   
                 GGTCCCTTTGCAGCCAATGCAACTTGGACGTTTTTGGGGTCTCCG 
               
               
                   
                 GACACCATCTCTATGTCTTGGCCCTGATCCTGAGCCGCCCGGGGC 
               
               
                   
                 TCCTGGTCTTCCGCCTCCTCGTCCTCGTCCTCTTCCCCGTCCTCG 
               
               
                   
                 TCCATGGTTATCACCCCCTCTTCTTTGAGGTCCACTGCCGCCGGA 
               
               
                   
                 GCCTTCTGGTCCAGATGTGTCTCCCTTCTCTCCTAGGCCATTTCC 
               
               
                   
                 AGGTCCTGTACCTGGCCCCTCGTCAGACATGATTCACACTAAAAG 
               
               
                   
                 AGATCAATAGACATCTTTATTAGACGACGCTCAGTGAATACAGGG 
               
               
                   
                 AGTGCAGACTCCTGCCCCCTCCAACAGCCCCCCCACCCTCATCCC 
               
               
                   
                 CTTCATGGTCGCTGTCAGACAGATCCAGGTCTGAAAATTCCCCAT 
               
               
                   
                 CCTCCGAACCATCCTCGTCCTCATCACCAATTACTCGCAGCCCGG 
               
               
                   
                 AAAACTCCCGCTGAACATCCTCAAGATTTGCGTCCTGAGCCTCAA 
               
               
                   
                 GCCAGGCCTCAAATTCCTCGTCCCCCTTTTTGCTGGACGGTAGGG 
               
               
                   
                 ATGGGGATTCTCGGGACCCCTCCTCTTCCTCTTCAAGGTCACCAG 
               
               
                   
                 ACAGAGATGCTACTGGGGCAACGGAAGAAAAGCTGGGTGCGGCCT 
               
               
                   
                 GTGAGGATCAGCTTATCGATGATAAGCTGTCAAACATGAGAATTC 
               
               
                   
                 TTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAA 
               
               
                   
                 TGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCG 
               
               
                   
                 GGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACA 
               
               
                   
                 TTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCT 
               
               
                   
                 TCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCG 
               
               
                   
                 TGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTT 
               
               
                   
                 TGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCA 
               
               
                   
                 GTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGG 
               
               
                   
                 TAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT 
               
               
                   
                 GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGT 
               
               
                   
                 TGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCA 
               
               
                   
                 GAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTAC 
               
               
                   
                 GGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCAT 
               
               
                   
                 GAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGG 
               
               
                   
                 ACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGT 
               
               
                   
                 AACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACC 
               
               
                   
                 AAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAAC 
               
               
                   
                 GTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCG 
               
               
                   
                 GCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC 
               
               
                   
                 ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAA 
               
               
                   
                 ATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACT 
               
               
                   
                 GGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGAC 
               
               
                   
                 GGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGA 
               
               
                   
                 GATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGT 
               
               
                   
                 TTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATT 
               
               
                   
                 TAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAA 
               
               
                   
                 AATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGT 
               
               
                   
                 AGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGT 
               
               
                   
                 AATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGT 
               
               
                   
                 TTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAC 
               
               
                   
                 TGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTA 
               
               
                   
                 GCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC 
               
               
                   
                 ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG 
               
               
                   
                 CGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACC 
               
               
                   
                 GGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACA 
               
               
                   
                 GCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACA 
               
               
                   
                 GCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGC 
               
               
                   
                 GGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCAC 
               
               
                   
                 GAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGT 
               
               
                   
                 CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTC 
               
               
                   
                 GTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTT 
               
               
                   
                 TTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT 
               
               
                   
                 TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTT 
               
               
                   
                 TGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAG 
               
               
                   
                 CGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTT 
               
               
                   
                 TCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCAC 
               
               
                   
                 TCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCTGTG 
               
               
                   
                 GAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGC 
               
               
                   
                 AGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAG 
               
               
                   
                 GTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAG 
               
               
                   
                 CATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCC 
               
               
                   
                 GCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCC 
               
               
                   
                 CCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGC 
               
               
                   
                 CTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGG 
               
               
                   
                 AGGCCTAGGCTTTTGCAAAAAGCTTGCATGCCTGCAGGTCGGCCG 
               
               
                   
                 CCACGACCGGTGCCGCCACCATCCCCTGACCCACGCCCCTGACCC 
               
               
                   
                 CTCACAAGGAGACGACCTTCCATGACCGAGTACAAGCCCACGGTG 
               
               
                   
                 CGCCTCGCCACCCGCGACGACGTCCCCCGGGCCGTACGCACCCTC 
               
               
                   
                 GCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGAC 
               
               
                   
                 CCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTC 
               
               
                   
                 CTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGAC 
               
               
                   
                 GACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAA 
               
               
                   
                 GCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTG 
               
               
                   
                 AGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTG 
               
               
                   
                 GCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTC 
               
               
                   
                 GGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTC 
               
               
                   
                 GTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCC 
               
               
                   
                 TTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGG 
               
               
                   
                 CTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCG 
               
               
                   
                 CGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACGCCCGCCC 
               
               
                   
                 CACGACCCGCAGCGCCCGACCGAAAGGAGCGCACGACCCCATGGC 
               
               
                   
                 TCCGACCGAAGCCGACCCGGGCGGCCCCGCCGACCCCGCACCCGC 
               
               
                   
                 CCCCGAGGCCCACCGACTCTAGAGGATCATAATCAGCCATACCAC 
               
               
                   
                 ATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCC 
               
               
                   
                 CCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTT 
               
               
                   
                 GTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCAC 
               
               
                   
                 AAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGG 
               
               
                   
                 TTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCACTCG 
               
               
                   
                 CCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGG 
               
               
                   
                 AATAGGGGAGATGGGGGAGGCTAACTGAAACACGGAAGGAGACAA 
               
               
                   
                 TACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATA 
               
               
                   
                 AAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGG 
               
               
                   
                 TCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCATTGG 
               
               
                   
                 GGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCA 
               
               
                   
                 AGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCA 
               
               
                   
                 GGCCCTGCCATAGCCACTGGCCCCGTGGGTTAGGGACGGGGTCCC 
               
               
                   
                 CCATGGGGAATGGTTTATGGTTCGTGGGGGTTATTATTTTGGGCG 
               
               
                   
                 TTGCGTGGGGTCTGGTCCACGACTGGACTGAGCAGACAGACCCAT 
               
               
                   
                 GGTTTTTGGATGGCCTGGGCATGGACCGCATGTACTGGCGCGACA 
               
               
                   
                 CGAACACCGGGCGTCTGTGGCTGCCAAACACCCCCGACCCCCAAA 
               
               
                   
                 AACCACCGCGCGGATTTCTGGCGTGCCAAGCTAGTCGACCAATTC 
               
               
                   
                 TCATGTTTGACAGCTTATCATCGCAGATCCGGGCAACGTTGTTGC 
               
               
                   
                 ATTGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTCTAGAAG 
               
               
                   
                 CTGGGTACCAGCTGCTAGCAAGCTTGCTAGCGGCCGGCTCGAGTT 
               
               
                   
                 TACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTA 
               
               
                   
                 TCAGTGATAGAGAACGATGTCGAGTTTACTCCCTATCAGTGATAG 
               
               
                   
                 AGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTAT 
               
               
                   
                 GTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTT 
               
               
                   
                 ATCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATC 
               
               
                   
                 AGTGATAGAGAACGTATGTCGAGGTAGGCGTGTACGGTGGGAGGC 
               
               
                   
                 CTATATAAGCAGAGCTCGTT 
               
               
                   
                 (SEQ ID NO: 41) 
               
               
                   
                   
               
               
                   
                 LINE-1 ORF2-NLS mRNA (SEQ ID NO: 42) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGA 
               
               
                   
                 AGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATA 
               
               
                   
                 GTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTT 
               
               
                   
                 AACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCC 
               
               
                   
                 AGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAG 
               
               
                   
                 ACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGA 
               
               
                   
                 TGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGA 
               
               
                   
                 GTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAA 
               
               
                   
                 ATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGC 
               
               
                   
                 ATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAAC 
               
               
                   
                 ACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAG 
               
               
                   
                 CGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACA 
               
               
                   
                 CCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAA 
               
               
                   
                 GACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATT 
               
               
                   
                 GATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTC 
               
               
                   
                 TTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTC 
               
               
                   
                 GGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATT 
               
               
                   
                 ACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGA 
               
               
                   
                 ATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAAT 
               
               
                   
                 AACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCA 
               
               
                   
                 GAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACC 
               
               
                   
                 TATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAG 
               
               
                   
                 TTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAG 
               
               
                   
                 ATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAA 
               
               
                   
                 CAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATT 
               
               
                   
                 CGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAA 
               
               
                   
                 ATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATA 
               
               
                   
                 GACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAAC 
               
               
                   
                 CAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGAC 
               
               
                   
                 CCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTG 
               
               
                   
                 TATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTG 
               
               
                   
                 GATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCC 
               
               
                   
                 CTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAAC 
               
               
                   
                 TCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCT 
               
               
                   
                 GAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTC 
               
               
                   
                 AAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCC 
               
               
                   
                 TTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGAT 
               
               
                   
                 ACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATC 
               
               
                   
                 GACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAA 
               
               
                   
                 CATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCT 
               
               
                   
                 GGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATT 
               
               
                   
                 CAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCT 
               
               
                   
                 ATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATG 
               
               
                   
                 CTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAG 
               
               
                   
                 ATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTT 
               
               
                   
                 AACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGC 
               
               
                   
                 CAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAG 
               
               
                   
                 GTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATA 
               
               
                   
                 CAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGAT 
               
               
                   
                 ATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTT 
               
               
                   
                 CTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATT 
               
               
                   
                 AACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAG 
               
               
                   
                 ACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGC 
               
               
                   
                 AAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAA 
               
               
                   
                 GATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAG 
               
               
                   
                 GAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGC 
               
               
                   
                 AGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATAT 
               
               
                   
                 CGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACG 
               
               
                   
                 GAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGA 
               
               
                   
                 GCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGT 
               
               
                   
                 GGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTA 
               
               
                   
                 ACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAG 
               
               
                   
                 TGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAAT 
               
               
                   
                 TACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAA 
               
               
                   
                 GACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATA 
               
               
                   
                 TGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAA 
               
               
                   
                 ATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACT 
               
               
                   
                 ATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATA 
               
               
                   
                 GGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCC 
               
               
                   
                 ACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGC 
               
               
                   
                 TTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCC 
               
               
                   
                 ACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGG 
               
               
                   
                 TTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAG 
               
               
                   
                 AAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGG 
               
               
                   
                 CATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAG 
               
               
                   
                 AAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACG 
               
               
                   
                 ACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAG 
               
               
                   
                 AAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGC 
               
               
                   
                 ACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTT 
               
               
                   
                 TGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATT 
               
               
                   
                 CCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAA 
               
               
                   
                 TACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATC 
               
               
                   
                 GCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGC 
               
               
                   
                 CCCACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACC 
               
               
                   
                 ATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTC 
               
               
                   
                 GTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTG 
               
               
                   
                 TCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGT 
               
               
                   
                 GGTAACGACTACAAAGACGATGACGACAAGCCCGCCGCCAAGAGG 
               
               
                   
                 GTGAAGCTGGACTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCAC 
               
               
                   
                 TGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAA 
               
               
                   
                 CCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAAC 
               
               
                   
                 TATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCT 
               
               
                   
                 TTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCC 
               
               
                   
                 TTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCC 
               
               
                   
                 GTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCA 
               
               
                   
                 ACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCC 
               
               
                   
                 GGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATC 
               
               
                   
                 GCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGC 
               
               
                   
                 ACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCA 
               
               
                   
                 TGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCC 
               
               
                   
                 TTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCC 
               
               
                   
                 CGCTGAGAGACACAAAAAATTCCAACACACTATTGCAATGAAAAT 
               
               
                   
                 AAATTTCCTTTATTAGCCAGAAGTCAGATGCTCAAGGGGCTTCAT 
               
               
                   
                 GATGTCCCCATAATTTTTGGCAGAGGGAAAAAGATCTCAGTGGTA 
               
               
                   
                 TTTGTGAGCCAGGGCATTGGCCTTCTGATAGGCAGCCTGCACCTG 
               
               
                   
                 AGGAGTGCGGCCGCTTTACTTGTACAGCTCGTCCATGCCGAGAGT 
               
               
                   
                 GATCCCGGCGGCGGTCACGAACTCCAGCAGGACCATGTGATCGCG 
               
               
                   
                 CTTCTCGTTGGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTA 
               
               
                   
                 GTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTT 
               
               
                   
                 CTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTT 
               
               
                   
                 GTGGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTC 
               
               
                   
                 GGCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTT 
               
               
                   
                 GTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAG 
               
               
                   
                 CTCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGC 
               
               
                   
                 GCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTC 
               
               
                   
                 CTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTG 
               
               
                   
                 CTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGT 
               
               
                   
                 CAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGT 
               
               
                   
                 GGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCC 
               
               
                   
                 CTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCC 
               
               
                   
                 GTCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTC 
               
               
                   
                 GCCCTTGCTCACCATGGTGGCGGGATCTGACGGTTCACTAAACCA 
               
               
                   
                 GCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATT 
               
               
                   
                 TGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGT 
               
               
                   
                 TGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGG 
               
               
                   
                 AGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTG 
               
               
                   
                 ATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAAT 
               
               
                   
                 ACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTAC 
               
               
                   
                 TGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATA 
               
               
                   
                 GGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGG 
               
               
                   
                 GCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTC 
               
               
                   
                 CCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAAT 
               
               
                   
                 GGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTA 
               
               
                   
                 AGTTATGTAACGGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGT 
               
               
                   
                 CTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCC 
               
               
                   
                 TCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCA 
               
               
                   
                 GCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCAC 
               
               
                   
                 AACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGA 
               
               
                   
                 TGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGT 
               
               
                   
                 T 
               
               
                   
                 (SEQ ID NO: 42) 
               
               
                   
                   
               
               
                   
                 LINE-1 alu mRNA GFP (SEQ ID NO: 43) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGA 
               
               
                   
                 AGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATA 
               
               
                   
                 GTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTT 
               
               
                   
                 AACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCC 
               
               
                   
                 AGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAG 
               
               
                   
                 ACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGA 
               
               
                   
                 TGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGA 
               
               
                   
                 GTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAA 
               
               
                   
                 ATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGC 
               
               
                   
                 ATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAAC 
               
               
                   
                 ACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAG 
               
               
                   
                 CGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACA 
               
               
                   
                 CCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAA 
               
               
                   
                 GACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATT 
               
               
                   
                 GATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTC 
               
               
                   
                 TTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTC 
               
               
                   
                 GGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATT 
               
               
                   
                 ACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGA 
               
               
                   
                 ATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAAT 
               
               
                   
                 AACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCA 
               
               
                   
                 GAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACC 
               
               
                   
                 TATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAG 
               
               
                   
                 TTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAG 
               
               
                   
                 ATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAA 
               
               
                   
                 CAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATT 
               
               
                   
                 CGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAA 
               
               
                   
                 ATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATA 
               
               
                   
                 GACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAAC 
               
               
                   
                 CAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGAC 
               
               
                   
                 CCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTG 
               
               
                   
                 TATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTG 
               
               
                   
                 GATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCC 
               
               
                   
                 CTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAAC 
               
               
                   
                 TCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCT 
               
               
                   
                 GAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTC 
               
               
                   
                 AAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCC 
               
               
                   
                 TTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGAT 
               
               
                   
                 ACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATC 
               
               
                   
                 GACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAA 
               
               
                   
                 CATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCT 
               
               
                   
                 GGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATT 
               
               
                   
                 CAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCT 
               
               
                   
                 ATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATG 
               
               
                   
                 CTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAG 
               
               
                   
                 ATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTT 
               
               
                   
                 AACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGC 
               
               
                   
                 CAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAG 
               
               
                   
                 GTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATA 
               
               
                   
                 CAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGAT 
               
               
                   
                 ATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTT 
               
               
                   
                 CTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATT 
               
               
                   
                 AACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAG 
               
               
                   
                 ACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGC 
               
               
                   
                 AAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAA 
               
               
                   
                 GATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAG 
               
               
                   
                 GAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGC 
               
               
                   
                 AGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATAT 
               
               
                   
                 CGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACG 
               
               
                   
                 GAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGA 
               
               
                   
                 GCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGT 
               
               
                   
                 GGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTA 
               
               
                   
                 ACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAG 
               
               
                   
                 TGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAAT 
               
               
                   
                 TACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAA 
               
               
                   
                 GACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATA 
               
               
                   
                 TGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAA 
               
               
                   
                 ATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACT 
               
               
                   
                 ATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATA 
               
               
                   
                 GGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCC 
               
               
                   
                 ACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGC 
               
               
                   
                 TTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCC 
               
               
                   
                 ACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGG 
               
               
                   
                 TTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAG 
               
               
                   
                 AAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGG 
               
               
                   
                 CATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAG 
               
               
                   
                 AAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACG 
               
               
                   
                 ACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAG 
               
               
                   
                 AAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGC 
               
               
                   
                 ACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTT 
               
               
                   
                 TGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATT 
               
               
                   
                 CCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAA 
               
               
                   
                 TACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATC 
               
               
                   
                 GCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGC 
               
               
                   
                 CCCACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACC 
               
               
                   
                 ATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTC 
               
               
                   
                 GTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTG 
               
               
                   
                 TCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGT 
               
               
                   
                 GGTAACGACTACAAAGACGATGACGACAAGTAAAGCGGCCGGGCG 
               
               
                   
                 CGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCG 
               
               
                   
                 GGAGGATCGCAGTTCGAGACCAGCGCGAGACCCCGTCTCTACAAA 
               
               
                   
                 AATACAAAAATTAGCTTCTAGAAGTTGTCTCCTCCTGCACTGACT 
               
               
                   
                 GACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCT 
               
               
                   
                 GGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGT 
               
               
                   
                 TGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTA 
               
               
                   
                 TCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTA 
               
               
                   
                 TAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGT 
               
               
                   
                 CAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCC 
               
               
                   
                 CACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGAC 
               
               
                   
                 TTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGC 
               
               
                   
                 CTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGA 
               
               
                   
                 CAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCT 
               
               
                   
                 GCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTG 
               
               
                   
                 CTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCTG 
               
               
                   
                 AGAGACACAAAAAATTCCAACACACTATTGCAATGAAAATAAATT 
               
               
                   
                 TCCTTTATTAGCCAGAAGTCAGATGCTCAAGGGGCTTCATGATGT 
               
               
                   
                 CCCCATAATTTTTGGCAGAGGGAAAAAGATCTCAGTGGTATTTGT 
               
               
                   
                 GAGCCAGGGCATTGGCCTTCTGATAGGCAGCCTGCACCTGAGGAG 
               
               
                   
                 TGCGGCCGCTTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCC 
               
               
                   
                 CGGCGGCGGTCACGAACTCCAGCAGGACCATGTGATCGCGCTTCT 
               
               
                   
                 CGTTGGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGT 
               
               
                   
                 TGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCT 
               
               
                   
                 GGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGC 
               
               
                   
                 GGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCA 
               
               
                   
                 TGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTGTGCC 
               
               
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGA 
               
               
                   
                 TGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGG 
               
               
                   
                 TCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGA 
               
               
                   
                 CGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCA 
               
               
                   
                 TGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGG 
               
               
                   
                 TGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGC 
               
               
                   
                 AGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGC 
               
               
                   
                 CCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCA 
               
               
                   
                 GCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCT 
               
               
                   
                 TGCTCACCATGGTGGCGGGATCTGACGGTTCACTAAACCAGCTCT 
               
               
                   
                 GCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGT 
               
               
                   
                 CAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATT 
               
               
                   
                 TTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACT 
               
               
                   
                 TGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTA 
               
               
                   
                 CTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTA 
               
               
                   
                 GATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGC 
               
               
                   
                 ATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGG 
               
               
                   
                 CGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGT 
               
               
                   
                 TTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTAT 
               
               
                   
                 TGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCG 
               
               
                   
                 GGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTA 
               
               
                   
                 TGTAACGGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCG 
               
               
                   
                 CCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCC 
               
               
                   
                 GCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCA 
               
               
                   
                 CAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTA 
               
               
                   
                 GAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT 
               
               
                   
                 (SEQ ID NO: 43) 
               
               
                   
                   
               
               
                   
                 LINE-1 plasmid CVBE IRES GFP (SEQ ID NO: 44) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGTGAGACAGTTAAAACAGCCTGTGGGTTGATC 
               
               
                   
                 CCACCCACAGGCCCATTGGGCGCTAGCACTCTGGTATCACGGTAC 
               
               
                   
                 CTTTGTGCGCCTGTTTTATACCCCCTCCCCCAACTGTAACTTAGA 
               
               
                   
                 AGTAACACACACCGATCAACAGTCAGCGTGGCACACCAGCCACGT 
               
               
                   
                 TTTGATCAAGCACTTCTGTTACCCCGGACTGAGTATCAATAGACT 
               
               
                   
                 GCTCACGCGGTTGAAGGAGAAAGCGTTCGTTATCCGGCCAACTAC 
               
               
                   
                 TTCGAAAAACCTAGTAACACCGTGGAAGTTGCAGAGTGTTTCGCT 
               
               
                   
                 CAGCACTACCCCAGTGTAGATCAGGTCGATGAGTCACCGCATTCC 
               
               
                   
                 CCACGGGCGACCGTGGCGGTGGCTGCGTTGGCGGCCTGCCCATGG 
               
               
                   
                 GGAAACCCATGGGACGCTCTAATACAGACATGGTGCGAAGAGTCT 
               
               
                   
                 ATTGAGCTAGTTGGTAGTCCTCCGGCCCCTGAATGCGGCTAATCC 
               
               
                   
                 TAACTGCGGAGCACACACCCTCAAGCCAGAGGGCAGTGTGTCGTA 
               
               
                   
                 ACGGGCAACTCTGCAGCGGAACCGACTACTTTGGGTGTCCGTGTT 
               
               
                   
                 TCATTTTATTCCTATACTGGCTGCTTATGGTGACAATTGAGAGAT 
               
               
                   
                 CGTTACCATATAGCTATTGGATTGGCCATCCGGTGACTAATAGAG 
               
               
                   
                 CTATTATATATCCCTTTGTTGGGTTTATACCACTTAGCTTGAAAG 
               
               
                   
                 AGGTTAAAACATTACAATTCATTGTTAAGTTGAATACAGCAAATA 
               
               
                   
                 CATGACCGGCTCTAACTCACATATCACCATCCTTACACTTAACAT 
               
               
                   
                 TAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTG 
               
               
                   
                 GATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCA 
               
               
                   
                 CCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCG 
               
               
                   
                 AAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGC 
               
               
                   
                 AATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAA 
               
               
                   
                 GCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACA 
               
               
                   
                 GCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGG 
               
               
                   
                 CGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGA 
               
               
                   
                 TCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATT 
               
               
                   
                 GAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACAC 
               
               
                   
                 GCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATAT 
               
               
                   
                 TTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAG 
               
               
                   
                 CGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTC 
               
               
                   
                 AAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAA 
               
               
                   
                 TTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAA 
               
               
                   
                 GAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCT 
               
               
                   
                 GCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGAT 
               
               
                   
                 TAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTATCA 
               
               
                   
                 AAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCAT 
               
               
                   
                 CGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGA 
               
               
                   
                 TACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGAC 
               
               
                   
                 CCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGC 
               
               
                   
                 CGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAA 
               
               
                   
                 CGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAG 
               
               
                   
                 ACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGAT 
               
               
                   
                 TGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCGAC 
               
               
                   
                 CGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTGTATGC 
               
               
                   
                 TAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATAC 
               
               
                   
                 CTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAA 
               
               
                   
                 CCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCCCT 
               
               
                   
                 GCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTT 
               
               
                   
                 TTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCT 
               
               
                   
                 CTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTA 
               
               
                   
                 CGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCAC 
               
               
                   
                 AAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCGACGC 
               
               
                   
                 TAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACATAT 
               
               
                   
                 TAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCAT 
               
               
                   
                 GCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAACA 
               
               
                   
                 CATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTATAGA 
               
               
                   
                 CGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAA 
               
               
                   
                 GACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAGATTAT 
               
               
                   
                 TCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTTAACGG 
               
               
                   
                 CCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGG 
               
               
                   
                 CTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCT 
               
               
                   
                 GGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATACAGCT 
               
               
                   
                 CGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGAT 
               
               
                   
                 TGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAA 
               
               
                   
                 ACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATTAACGT 
               
               
                   
                 CCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGA 
               
               
                   
                 ATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAG 
               
               
                   
                 GATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAAGATTT 
               
               
                   
                 GTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGA 
               
               
                   
                 TACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAAT 
               
               
                   
                 CAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATATCGCTT 
               
               
                   
                 TAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCT 
               
               
                   
                 CGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAG 
               
               
                   
                 AATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGAT 
               
               
                   
                 TACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAA 
               
               
                   
                 GACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAA 
               
               
                   
                 TCGGACCGAACCATCAGAGATAATGCCCCACATCTATAATTACCT 
               
               
                   
                 TATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAG 
               
               
                   
                 CCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCG 
               
               
                   
                 GAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAA 
               
               
                   
                 CAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAA 
               
               
                   
                 GACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGT 
               
               
                   
                 CGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAA 
               
               
                   
                 GGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTG 
               
               
                   
                 TACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTAC 
               
               
                   
                 ATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGAT 
               
               
                   
                 AAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAAC 
               
               
                   
                 GAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTT 
               
               
                   
                 TAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTG 
               
               
                   
                 TAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCAT 
               
               
                   
                 GCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATC 
               
               
                   
                 TGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCT 
               
               
                   
                 GCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAA 
               
               
                   
                 ATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTT 
               
               
                   
                 CGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAA 
               
               
                   
                 GAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGGC 
               
               
                   
                 CTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCAC 
               
               
                   
                 AATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGA 
               
               
                   
                 GTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTTGG 
               
               
                   
                 GACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCA 
               
               
                   
                 GGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAA 
               
               
                   
                 CGACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAGAAGTTG 
               
               
                   
                 TCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCCG 
               
               
                   
                 CAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGAC 
               
               
                   
                 TGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGC 
               
               
                   
                 TGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTT 
               
               
                   
                 CATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGA 
               
               
                   
                 GGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGT 
               
               
                   
                 GTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTG 
               
               
                   
                 TCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCAC 
               
               
                   
                 GGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGC 
               
               
                   
                 TCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCT 
               
               
                   
                 GACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCT 
               
               
                   
                 GCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGC 
               
               
                   
                 GGACCTTCCTTCCCGCGAACAAACGACCCAACACCCGTGCGTTTT 
               
               
                   
                 ATTCTGTCTTTTTATTGCCGATCCCCTCAGAAGAACTCGTCAAGA 
               
               
                   
                 AGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCG 
               
               
                   
                 TAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCA 
               
               
                   
                 GCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCGGCC 
               
               
                   
                 GCTTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGC 
               
               
                   
                 GGTCACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGG 
               
               
                   
                 GTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGG 
               
               
                   
                 CAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTG 
               
               
                   
                 GTCGGCCAGGTGAGTCCAGGAGATGTTTCAGCACTGTTGCCTTTA 
               
               
                   
                 GTCTCGAGGCAACTTAGACAACTGAGTATTGATCTGAGCACAGCA 
               
               
                   
                 GGGTGTGAGCTGTTTGAAGATACTGGGGTTGGGGGTGAAGAAACT 
               
               
                   
                 GCAGAGGACTAACTGGGCTGAGACCCAGTGGCAATGTTTTAGGGC 
               
               
                   
                 CTAAGGAATGCCTCTGAAAATCTAGATGGACAACTTTGACTTTGA 
               
               
                   
                 GAAAAGAGAGGTGGAAATGAGGAAAATGACTTTTCTTTATTAGAT 
               
               
                   
                 TTCGGTAGAAAGAACTTTCATCTTTCCCCTATTTTTGTTATTCGT 
               
               
                   
                 TTTAAAACATCTATCTGGAGGCAGGACAAGTATGGTCATTAAAAA 
               
               
                   
                 GATGCAGGCAGAAGGCATATATTGGCTCAGTCAAAGTGGGGAACT 
               
               
                   
                 TTGGTGGCCAAACATACATTGCTAAGGCTATTCCTATATCAGCTG 
               
               
                   
                 GACACATATAAAATGCTGCTAATGCTTCATTACAAACTTATATCC 
               
               
                   
                 TTTAATTCCAGATGGGGGCAAAGTATGTCCAGGGGTGAGGAACAA 
               
               
                   
                 TTGAAACATTTGGGCTGGAGTAGATTTTGAAAGTCAGCTCTGTGT 
               
               
                   
                 GTGTGTGTGTGTGTGTGTGTGTGAGAGCGTGTGTTTCTTTTAACG 
               
               
                   
                 TTTTCAGCCTACAGCATACAGGGTTCATGGTGGCAAGAAGATAAC 
               
               
                   
                 AAGATTTAAATTATGGCCAGTGACTAGTGCTGCAAGAAGAACAAC 
               
               
                   
                 TACCTGCATTTAATGGGAAAGCAAAATCTCAGGCTTTGAGGGAAG 
               
               
                   
                 TTAACATAGGCTTGATTCTGGGTGGAAGCTGGGTGTGTAGTTATC 
               
               
                   
                 TGGAGGCCAGGCTGGAGCTCTCAGCTCACTATGGGTTCATCTTTA 
               
               
                   
                 TTGTCTCCTTTCATCTCAACAGCTGCACGCTGCCGTCCTCGATGT 
               
               
                   
                 TGTGGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGT 
               
               
                   
                 CGGCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCT 
               
               
                   
                 TGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCA 
               
               
                   
                 GCTCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGG 
               
               
                   
                 CGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCT 
               
               
                   
                 CCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                 GCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGG 
               
               
                   
                 TCAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGG 
               
               
                   
                 TGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGC 
               
               
                   
                 CCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGC 
               
               
                   
                 CGTCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCT 
               
               
                   
                 CGCCCTTGCTCACCATGGTGGCGAATTCGAAGCTTGAGCACGAGA 
               
               
                   
                 TCTGAGTCCGGTAGGCCTAGCGGATCTGACGGTTCACTAAACCAG 
               
               
                   
                 CTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTT 
               
               
                   
                 GCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTT 
               
               
                   
                 GATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGA 
               
               
                   
                 GACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGA 
               
               
                   
                 TGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATA 
               
               
                   
                 CGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACT 
               
               
                   
                 GGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAG 
               
               
                   
                 GGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGG 
               
               
                   
                 CAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCC 
               
               
                   
                 CTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATG 
               
               
                   
                 GGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAA 
               
               
                   
                 GTTATGTAACGGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTC 
               
               
                   
                 TTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCT 
               
               
                   
                 CCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAG 
               
               
                   
                 CTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA 
               
               
                   
                 ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT 
               
               
                   
                 GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT 
               
               
                   
                 AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG 
               
               
                   
                 GTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGT 
               
               
                   
                 ATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCT 
               
               
                   
                 CTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTG 
               
               
                   
                 CTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCT 
               
               
                   
                 GCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACG 
               
               
                   
                 GCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAA 
               
               
                   
                 ATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGAT 
               
               
                   
                 TTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAG 
               
               
                   
                 GGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCAT 
               
               
                   
                 TGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTC 
               
               
                   
                 AATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCG 
               
               
                   
                 CCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGA 
               
               
                   
                 CTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCT 
               
               
                   
                 GAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGC 
               
               
                   
                 TTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCA 
               
               
                   
                 CGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGA 
               
               
                   
                 CTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCG 
               
               
                   
                 GCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCT 
               
               
                   
                 GTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATC 
               
               
                   
                 GTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGT 
               
               
                   
                 TGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCC 
               
               
                   
                 GGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGT 
               
               
                   
                 ATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCC 
               
               
                   
                 GGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCG 
               
               
                   
                 AGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCT 
               
               
                   
                 GGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAG 
               
               
                   
                 GCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCA 
               
               
                   
                 CGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTT 
               
               
                   
                 TTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTA 
               
               
                   
                 TCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGG 
               
               
                   
                 CGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGC 
               
               
                   
                 TCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTT 
               
               
                   
                 CTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGT 
               
               
                   
                 TGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCAC 
               
               
                   
                 TCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTG 
               
               
                   
                 TCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGA 
               
               
                   
                 CAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGA 
               
               
                   
                 TGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTG 
               
               
                   
                 GCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTC 
               
               
                   
                 AGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATT 
               
               
                   
                 ACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCC 
               
               
                   
                 AGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTC 
               
               
                   
                 CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGG 
               
               
                   
                 TGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT 
               
               
                   
                 GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACC 
               
               
                   
                 GGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT 
               
               
                   
                 CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGC 
               
               
                   
                 TCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC 
               
               
                   
                 TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA 
               
               
                   
                 CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGC 
               
               
                   
                 AGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGG 
               
               
                   
                 CCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCT 
               
               
                   
                 CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGA 
               
               
                   
                 TCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGC 
               
               
                   
                 AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCT 
               
               
                   
                 TTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCA 
               
               
                   
                 CGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC 
               
               
                   
                 TAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGT 
               
               
                   
                 ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGT 
               
               
                   
                 GAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTT 
               
               
                   
                 GCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTT 
               
               
                   
                 CAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAA 
               
               
                   
                 CTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTG 
               
               
                   
                 GCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGA 
               
               
                   
                 TCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGAT 
               
               
                   
                 CTATCCGAGATCCGAGGAATATCGAAATCGGGGCGCGCCTGGTGT 
               
               
                   
                 ACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATCGT 
               
               
                   
                 TGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAG 
               
               
                   
                 TCCAATCGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTAC 
               
               
                   
                 CGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAACGTA 
               
               
                   
                 TAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATC 
               
               
                   
                 AGCAGGAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCT 
               
               
                   
                 TATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCC 
               
               
                   
                 AGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGC 
               
               
                   
                 GCGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCT 
               
               
                   
                 TGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTT 
               
               
                   
                 TAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGG 
               
               
                   
                 GCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTT 
               
               
                   
                 GGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGATGGCAT 
               
               
                   
                 GACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAA 
               
               
                   
                 CACTGCGGCCCGGCCGCAACTTACTTCTGACAACGATTGGAGGAC 
               
               
                   
                 CGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAA 
               
               
                   
                 CTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAA 
               
               
                   
                 ACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACCT 
               
               
                   
                 TGCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGC 
               
               
                   
                 AACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCAC 
               
               
                   
                 TTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAAT 
               
               
                   
                 CTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGG 
               
               
                   
                 GGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGG 
               
               
                   
                 GGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGA 
               
               
                   
                 TAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTGCAT 
               
               
                   
                 TGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACT 
               
               
                   
                 CCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTG 
               
               
                   
                 CCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGA 
               
               
                   
                 TCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGA 
               
               
                   
                 GCTTACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGA 
               
               
                   
                 ATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGATCGCGG 
               
               
                   
                 CTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCT 
               
               
                   
                 CTAGTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAA 
               
               
                   
                 CCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCAAT 
               
               
                   
                 CGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGAT 
               
               
                   
                 GGATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGAT 
               
               
                   
                 CTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGA 
               
               
                   
                 GTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACC 
               
               
                   
                 GCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCC 
               
               
                   
                 CATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGA 
               
               
                   
                 GTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCA 
               
               
                   
                 TATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCC 
               
               
                   
                 CGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTAC 
               
               
                   
                 TTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGAT 
               
               
                   
                 GCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTC 
               
               
                   
                 ACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTT 
               
               
                   
                 GTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAA 
               
               
                   
                 CTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGA 
               
               
                   
                 GGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCAGATC 
               
               
                   
                 TTTGTCGATCCTACCATCCACTCGACACACCCGCCAG 
               
               
                   
                 (SEQ ID NO: 44) 
               
               
                   
                   
               
               
                   
                 LINE-1 Plasmid EV71 IRES (SEQ ID NO: 45) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGTGAGACAGTTAAAACAGCTGTGGGTTGTCAC 
               
               
                   
                 CCACCCACAGGGTCCACTGGGCGCTAGTACACTGGTATCTCGGTA 
               
               
                   
                 CCTTTGTACGCCTGTTTTATACCCCCTCCCTGATTTGCAACTTAG 
               
               
                   
                 AAGCAACGCAAACCAGATCAATAGTAGGTGTGACATACCAGTCGC 
               
               
                   
                 ATCTTGATCAAGCACTTCTGTATCCCCGGACCGAGTATCAATAGA 
               
               
                   
                 CTGTGCACACGGTTGAAGGAGAAAACGTCCGTTACCCGGCTAACT 
               
               
                   
                 ACTTCGAGAAGCCTAGTAACGCCATTGAAGTTGCAGAGTGTTTCG 
               
               
                   
                 CTCAGCACTCCCCCCGTGTAGATCAGGTCGATGAGTCACCGCATT 
               
               
                   
                 CCCCACGGGCGACCGTGGCGGTGGCTGCGTTGGCGGCCTGCCTAT 
               
               
                   
                 GGGGTAACCCATAGGACGCTCTAATACGGACATGGCGTGAAGAGT 
               
               
                   
                 CTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGCTAAT 
               
               
                   
                 CCTAACTGCGGAGCACATACCCTTAATCCAAAGGGCAGTGTGTCG 
               
               
                   
                 TAACGGGCAACTCTGCAGCGGAACCGACTACTTTGGGTGTCCGTG 
               
               
                   
                 TTTCTTTTTATTCTTGTATTGGCTGCTTATGGTGACAATTAAAGA 
               
               
                   
                 ATTGTTACCATATAGCTATTGGATTGGCCATCCAGTGTCAAACAG 
               
               
                   
                 AGCTATTGTATATCTCTTTGTTGGATTCACACCTCTCACTCTTGA 
               
               
                   
                 AACGTTACACACCCTCAATTACATTATACTGCTGAACACGAAGCG 
               
               
                   
                 TACATGACCGGCTCTAACTCACATATCACCATCCTTACACTTAAC 
               
               
                   
                 ATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGC 
               
               
                   
                 TGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACC 
               
               
                   
                 CACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGG 
               
               
                   
                 CGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTC 
               
               
                   
                 GCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATT 
               
               
                   
                 AAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATA 
               
               
                   
                 CAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACC 
               
               
                   
                 GGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGA 
               
               
                   
                 GATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCA 
               
               
                   
                 TTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGAC 
               
               
                   
                 ACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGAT 
               
               
                   
                 ATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTC 
               
               
                   
                 AGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGC 
               
               
                   
                 TCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACA 
               
               
                   
                 AATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATC 
               
               
                   
                 AAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAAC 
               
               
                   
                 CTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAG 
               
               
                   
                 ATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTAT 
               
               
                   
                 CAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTC 
               
               
                   
                 ATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATC 
               
               
                   
                 GATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAG 
               
               
                   
                 ACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGC 
               
               
                   
                 GCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATT 
               
               
                   
                 AACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGAC 
               
               
                   
                 AGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAG 
               
               
                   
                 ATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCG 
               
               
                   
                 ACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTGTAT 
               
               
                   
                 GCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGAT 
               
               
                   
                 ACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTC 
               
               
                   
                 AACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCC 
               
               
                   
                 CTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAG 
               
               
                   
                 TTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAG 
               
               
                   
                 CTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTC 
               
               
                   
                 TACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACC 
               
               
                   
                 ACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCGAC 
               
               
                   
                 GCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACAT 
               
               
                   
                 ATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGC 
               
               
                   
                 ATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAA 
               
               
                   
                 CACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTATA 
               
               
                   
                 GACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTC 
               
               
                   
                 AAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAGATT 
               
               
                   
                 ATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTTAAC 
               
               
                   
                 GGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAA 
               
               
                   
                 GGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTG 
               
               
                   
                 CTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATACAG 
               
               
                   
                 CTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATG 
               
               
                   
                 ATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTT 
               
               
                   
                 AAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATTAAC 
               
               
                   
                 GTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACC 
               
               
                   
                 GAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAA 
               
               
                   
                 AGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAAGAT 
               
               
                   
                 TTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAA 
               
               
                   
                 GATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGA 
               
               
                   
                 ATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATATCGC 
               
               
                   
                 TTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAG 
               
               
                   
                 CTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCA 
               
               
                   
                 AGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGG 
               
               
                   
                 ATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACT 
               
               
                   
                 AAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGG 
               
               
                   
                 AATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAATTAC 
               
               
                   
                 CTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGAC 
               
               
                   
                 AGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGC 
               
               
                   
                 CGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAAATC 
               
               
                   
                 AACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATA 
               
               
                   
                 AAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGC 
               
               
                   
                 GTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACT 
               
               
                   
                 AAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTC 
               
               
                   
                 TGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACT 
               
               
                   
                 ACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTG 
               
               
                   
                 ATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAA 
               
               
                   
                 ACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCAT 
               
               
                   
                 TTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAG 
               
               
                   
                 TGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACC 
               
               
                   
                 ATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAA 
               
               
                   
                 TCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACC 
               
               
                   
                 CTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGG 
               
               
                   
                 AAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCC 
               
               
                   
                 TTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATAC 
               
               
                   
                 AAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCG 
               
               
                   
                 GCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCC 
               
               
                   
                 ACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATG 
               
               
                   
                 GAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTT 
               
               
                   
                 GGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCT 
               
               
                   
                 CAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGT 
               
               
                   
                 AACGACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAGAAGT 
               
               
                   
                 TGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATC 
               
               
                   
                 CGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTG 
               
               
                   
                 ACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATAC 
               
               
                   
                 GCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCT 
               
               
                   
                 TTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTAT 
               
               
                   
                 GAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACT 
               
               
                   
                 GTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACC 
               
               
                   
                 TGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCC 
               
               
                   
                 ACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGG 
               
               
                   
                 GCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAG 
               
               
                   
                 CTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATT 
               
               
                   
                 CTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCA 
               
               
                   
                 GCGGACCTTCCTTCCCGCGAACAAACGACCCAACACCCGTGCGTT 
               
               
                   
                 TTATTCTGTCTTTTTATTGCCGATCCCCTCAGAAGAACTCGTCAA 
               
               
                   
                 GAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATAC 
               
               
                   
                 CGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTT 
               
               
                   
                 CAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCGG 
               
               
                   
                 CCGCTTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCG 
               
               
                   
                 GCGGTCACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTG 
               
               
                   
                 GGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCG 
               
               
                   
                 GGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAG 
               
               
                   
                 TGGTCGGCCAGGTGAGTCCAGGAGATGTTTCAGCACTGTTGCCTT 
               
               
                   
                 TAGTCTCGAGGCAACTTAGACAACTGAGTATTGATCTGAGCACAG 
               
               
                   
                 CAGGGTGTGAGCTGTTTGAAGATACTGGGGTTGGGGGTGAAGAAA 
               
               
                   
                 CTGCAGAGGACTAACTGGGCTGAGACCCAGTGGCAATGTTTTAGG 
               
               
                   
                 GCCTAAGGAATGCCTCTGAAAATCTAGATGGACAACTTTGACTTT 
               
               
                   
                 GAGAAAAGAGAGGTGGAAATGAGGAAAATGACTTTTCTTTATTAG 
               
               
                   
                 ATTTCGGTAGAAAGAACTTTCATCTTTCCCCTATTTTTGTTATTC 
               
               
                   
                 GTTTTAAAACATCTATCTGGAGGCAGGACAAGTATGGTCATTAAA 
               
               
                   
                 AAGATGCAGGCAGAAGGCATATATTGGCTCAGTCAAAGTGGGGAA 
               
               
                   
                 CTTTGGTGGCCAAACATACATTGCTAAGGCTATTCCTATATCAGC 
               
               
                   
                 TGGACACATATAAAATGCTGCTAATGCTTCATTACAAACTTATAT 
               
               
                   
                 CCTTTAATTCCAGATGGGGGCAAAGTATGTCCAGGGGTGAGGAAC 
               
               
                   
                 AATTGAAACATTTGGGCTGGAGTAGATTTTGAAAGTCAGCTCTGT 
               
               
                   
                 GTGTGTGTGTGTGTGTGTGTGTGTGAGAGCGTGTGTTTCTTTTAA 
               
               
                   
                 CGTTTTCAGCCTACAGCATACAGGGTTCATGGTGGCAAGAAGATA 
               
               
                   
                 ACAAGATTTAAATTATGGCCAGTGACTAGTGCTGCAAGAAGAACA 
               
               
                   
                 ACTACCTGCATTTAATGGGAAAGCAAAATCTCAGGCTTTGAGGGA 
               
               
                   
                 AGTTAACATAGGCTTGATTCTGGGTGGAAGCTGGGTGTGTAGTTA 
               
               
                   
                 TCTGGAGGCCAGGCTGGAGCTCTCAGCTCACTATGGGTTCATCTT 
               
               
                   
                 TATTGTCTCCTTTCATCTCAACAGCTGCACGCTGCCGTCCTCGAT 
               
               
                   
                 GTTGTGGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTT 
               
               
                   
                 GTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAG 
               
               
                   
                 CTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTT 
               
               
                   
                 CAGCTCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTC 
               
               
                   
                 GGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCG 
               
               
                   
                 CTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTG 
               
               
                   
                 CTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTA 
               
               
                   
                 GGTCAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCC 
               
               
                   
                 GGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTC 
               
               
                   
                 GCCGTCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTC 
               
               
                   
                 CTCGCCCTTGCTCACCATGGTGGCGAATTCGAAGCTTGAGCACGA 
               
               
                   
                 GATCTGAGTCCGGTAGGCCTAGCGGATCTGACGGTTCACTAAACC 
               
               
                   
                 AGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCAT 
               
               
                   
                 TTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCG 
               
               
                   
                 TTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTG 
               
               
                   
                 GAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATT 
               
               
                   
                 GATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAA 
               
               
                   
                 TACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTA 
               
               
                   
                 CTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAAT 
               
               
                   
                 AGGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTG 
               
               
                   
                 GGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGT 
               
               
                   
                 CCCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAA 
               
               
                   
                 TGGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGT 
               
               
                   
                 AAGTTATGTAACGGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCG 
               
               
                   
                 TCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGC 
               
               
                   
                 CTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTC 
               
               
                   
                 AGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCA 
               
               
                   
                 CAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTG 
               
               
                   
                 ATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAG 
               
               
                   
                 TTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGG 
               
               
                   
                 AGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTG 
               
               
                   
                 GTATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGC 
               
               
                   
                 CTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGAT 
               
               
                   
                 TGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACG 
               
               
                   
                 CTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATA 
               
               
                   
                 CGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAG 
               
               
                   
                 AAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCG 
               
               
                   
                 ATTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACT 
               
               
                   
                 AGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTC 
               
               
                   
                 ATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATC 
               
               
                   
                 TCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCC 
               
               
                   
                 CGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCT 
               
               
                   
                 GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCT 
               
               
                   
                 CTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAG 
               
               
                   
                 GCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTG 
               
               
                   
                 CACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTAT 
               
               
                   
                 GACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTC 
               
               
                   
                 CGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGAC 
               
               
                   
                 CTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTA 
               
               
                   
                 TCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGAC 
               
               
                   
                 GTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTG 
               
               
                   
                 CCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAA 
               
               
                   
                 GTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGAT 
               
               
                   
                 CCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAG 
               
               
                   
                 CGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGAT 
               
               
                   
                 CTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCC 
               
               
                   
                 AGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACC 
               
               
                   
                 CACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGC 
               
               
                   
                 TTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGC 
               
               
                   
                 TATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTT 
               
               
                   
                 GGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCC 
               
               
                   
                 GCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAG 
               
               
                   
                 TTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCT 
               
               
                   
                 GTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCC 
               
               
                   
                 ACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCAT 
               
               
                   
                 TGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAG 
               
               
                   
                 GACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGG 
               
               
                   
                 GATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACT 
               
               
                   
                 TGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCG 
               
               
                   
                 TCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGA 
               
               
                   
                 TTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGG 
               
               
                   
                 CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC 
               
               
                   
                 TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA 
               
               
                   
                 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC 
               
               
                   
                 CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA 
               
               
                   
                 CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT 
               
               
                   
                 CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC 
               
               
                   
                 GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC 
               
               
                   
                 GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 
               
               
                   
                 GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA 
               
               
                   
                 GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT 
               
               
                   
                 GGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCG 
               
               
                   
                 CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT 
               
               
                   
                 GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT 
               
               
                   
                 GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATC 
               
               
                   
                 CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT 
               
               
                   
                 CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA 
               
               
                   
                 CCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA 
               
               
                   
                 GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCA 
               
               
                   
                 GTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAG 
               
               
                   
                 TTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATC 
               
               
                   
                 TTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACG 
               
               
                   
                 AACTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTTGGCTATTGCT 
               
               
                   
                 TGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGA 
               
               
                   
                 GATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCG 
               
               
                   
                 ATCTATCCGAGATCCGAGGAATATCGAAATCGGGGCGCGCCTGGT 
               
               
                   
                 GTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATC 
               
               
                   
                 GTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGA 
               
               
                   
                 AGTCCAATCGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGT 
               
               
                   
                 ACCGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAACG 
               
               
                   
                 TATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGA 
               
               
                   
                 TCAGCAGGAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCC 
               
               
                   
                 CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC 
               
               
                   
                 CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT 
               
               
                   
                 GCGCGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC 
               
               
                   
                 CTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGCACT 
               
               
                   
                 TTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC 
               
               
                   
                 GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC 
               
               
                   
                 TTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGATGGC 
               
               
                   
                 ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT 
               
               
                   
                 AACACTGCGGCCCGGCCGCAACTTACTTCTGACAACGATTGGAGG 
               
               
                   
                 ACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGT 
               
               
                   
                 AACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACC 
               
               
                   
                 AAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAAC 
               
               
                   
                 CTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCG 
               
               
                   
                 GCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC 
               
               
                   
                 ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAA 
               
               
                   
                 ATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACT 
               
               
                   
                 GGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGAC 
               
               
                   
                 GGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGA 
               
               
                   
                 GATAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTGC 
               
               
                   
                 ATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATA 
               
               
                   
                 CTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACT 
               
               
                   
                 TGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAA 
               
               
                   
                 GATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTC 
               
               
                   
                 GAGCTTACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATC 
               
               
                   
                 GAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGATCGC 
               
               
                   
                 GGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCC 
               
               
                   
                 CTCTAGTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAA 
               
               
                   
                 AACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCA 
               
               
                   
                 ATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCG 
               
               
                   
                 ATGGATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAG 
               
               
                   
                 ATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG 
               
               
                   
                 GAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGA 
               
               
                   
                 CCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTT 
               
               
                   
                 CCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTG 
               
               
                   
                 GAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTAT 
               
               
                   
                 CATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGG 
               
               
                   
                 CCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCT 
               
               
                   
                 ACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTG 
               
               
                   
                 ATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGAC 
               
               
                   
                 TCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGT 
               
               
                   
                 TTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAAC 
               
               
                   
                 AACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGG 
               
               
                   
                 GAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCAGA 
               
               
                   
                 TCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAG 
               
               
                   
                 (SEQ ID NO: 45) 
               
               
                   
                   
               
               
                   
                 LINE-1 plasmid ORF1-E2A-ORF2 GFP 
               
               
                   
                 (SEQ ID NO: 46) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGGGAAGCGGACAGTGTACTAATTATGCTCTCT 
               
               
                   
                 TGAAATTGGCTGGAGATGTTGAGAGCAACCCTGGACCTATGACCG 
               
               
                   
                 GCTCTAACTCACATATCACCATCCTTACACTTAACATTAACGGCC 
               
               
                   
                 TCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAAT 
               
               
                   
                 CACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCT 
               
               
                   
                 GTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTT 
               
               
                   
                 ATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGG 
               
               
                   
                 TCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATA 
               
               
                   
                 AGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAG 
               
               
                   
                 AACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTC 
               
               
                   
                 GCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATT 
               
               
                   
                 CTCATACGTTGATTATGGGTGATTTCAATACACCATTGAGCACCC 
               
               
                   
                 TGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGC 
               
               
                   
                 TCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCA 
               
               
                   
                 CTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCAC 
               
               
                   
                 ATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTC 
               
               
                   
                 TGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGT 
               
               
                   
                 CAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGA 
               
               
                   
                 CCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCA 
               
               
                   
                 ACGATTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGT 
               
               
                   
                 TCTTCGAAACAAATGAGAATAAGGATACTACCTATCAAAACCTTT 
               
               
                   
                 GGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCA 
               
               
                   
                 ACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCA 
               
               
                   
                 CCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCA 
               
               
                   
                 AGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGA 
               
               
                   
                 AAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTC 
               
               
                   
                 GTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGG 
               
               
                   
                 CACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCA 
               
               
                   
                 TCAAGAACGACAAGGGCGACATCACTACTGACCCGACCGAGATCC 
               
               
                   
                 AGACCACTATTCGGGAGTATTATAAGCATTTGTATGCTAACAAGC 
               
               
                   
                 TTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTC 
               
               
                   
                 TGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAA 
               
               
                   
                 TTACAGGAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAA 
               
               
                   
                 AGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAAC 
               
               
                   
                 GGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGT 
               
               
                   
                 CTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTT 
               
               
                   
                 CTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGG 
               
               
                   
                 AAAACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATAT 
               
               
                   
                 TGAACAAGATTCTCGCCAACAGAATCCAACAACATATTAAGAAAT 
               
               
                   
                 TGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCT 
               
               
                   
                 GGTTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATA 
               
               
                   
                 GAGCTAAGGATAAGAATCATATGATCATCTCTATAGACGCGGAAA 
               
               
                   
                 AGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGA 
               
               
                   
                 ACAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAA 
               
               
                   
                 TTTACGATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGC 
               
               
                   
                 TCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCC 
               
               
                   
                 TCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGG 
               
               
                   
                 CTATTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGG 
               
               
                   
                 AAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACC 
               
               
                   
                 TGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTT 
               
               
                   
                 CTAACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAAT 
               
               
                   
                 CTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGA 
               
               
                   
                 TAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGT 
               
               
                   
                 ATCTCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGG 
               
               
                   
                 AAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATA 
               
               
                   
                 AGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAG 
               
               
                   
                 TGAAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCA 
               
               
                   
                 TCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAA 
               
               
                   
                 CAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGA 
               
               
                   
                 AGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGC 
               
               
                   
                 CTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCT 
               
               
                   
                 GGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCG 
               
               
                   
                 AACCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCG 
               
               
                   
                 ATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCA 
               
               
                   
                 ACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCA 
               
               
                   
                 AGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGT 
               
               
                   
                 GGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGG 
               
               
                   
                 AAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAG 
               
               
                   
                 ATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGA 
               
               
                   
                 TTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCA 
               
               
                   
                 AGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAA 
               
               
                   
                 AGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAA 
               
               
                   
                 TATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATC 
               
               
                   
                 CCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAG 
               
               
                   
                 AGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAA 
               
               
                   
                 GCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATACC 
               
               
                   
                 ACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATA 
               
               
                   
                 ATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATT 
               
               
                   
                 GCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCT 
               
               
                   
                 GGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCG 
               
               
                   
                 CAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTT 
               
               
                   
                 GTTACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTA 
               
               
                   
                 CGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCG 
               
               
                   
                 ATTGGATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACG 
               
               
                   
                 CAGCAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGA 
               
               
                   
                 TGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAA 
               
               
                   
                 AGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACA 
               
               
                   
                 AAGACGATGACGACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCC 
               
               
                   
                 TGCACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTA 
               
               
                   
                 ATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTC 
               
               
                   
                 TTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAA 
               
               
                   
                 TGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCT 
               
               
                   
                 CCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGT 
               
               
                   
                 GGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTG 
               
               
                   
                 ACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCC 
               
               
                   
                 TTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAAC 
               
               
                   
                 TCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGT 
               
               
                   
                 TGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCT 
               
               
                   
                 TTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGA 
               
               
                   
                 CGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTC 
               
               
                   
                 CTTCCCGCGAACAAACGACCCAACACCCGTGCGTTTTATTCTGTC 
               
               
                   
                 TTTTTATTGCCGATCCCCTCAGAAGAACTCGTCAAGAAGGCGATA 
               
               
                   
                 GAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCAC 
               
               
                   
                 GAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATC 
               
               
                   
                 ACGGGTAGCCAACGCTATGTCCTGATAGCGGTCGGCCGCTTTACT 
               
               
                   
                 TGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGA 
               
               
                   
                 ACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGC 
               
               
                   
                 TCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCA 
               
               
                   
                 CGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCCA 
               
               
                   
                 GGTGAGTCCAGGAGATGTTTCAGCACTGTTGCCTTTAGTCTCGAG 
               
               
                   
                 GCAACTTAGACAACTGAGTATTGATCTGAGCACAGCAGGGTGTGA 
               
               
                   
                 GCTGTTTGAAGATACTGGGGTTGGGGGTGAAGAAACTGCAGAGGA 
               
               
                   
                 CTAACTGGGCTGAGACCCAGTGGCAATGTTTTAGGGCCTAAGGAA 
               
               
                   
                 TGCCTCTGAAAATCTAGATGGACAACTTTGACTTTGAGAAAAGAG 
               
               
                   
                 AGGTGGAAATGAGGAAAATGACTTTTCTTTATTAGATTTCGGTAG 
               
               
                   
                 AAAGAACTTTCATCTTTCCCCTATTTTTGTTATTCGTTTTAAAAC 
               
               
                   
                 ATCTATCTGGAGGCAGGACAAGTATGGTCATTAAAAAGATGCAGG 
               
               
                   
                 CAGAAGGCATATATTGGCTCAGTCAAAGTGGGGAACTTTGGTGGC 
               
               
                   
                 CAAACATACATTGCTAAGGCTATTCCTATATCAGCTGGACACATA 
               
               
                   
                 TAAAATGCTGCTAATGCTTCATTACAAACTTATATCCTTTAATTC 
               
               
                   
                 CAGATGGGGGCAAAGTATGTCCAGGGGTGAGGAACAATTGAAACA 
               
               
                   
                 TTTGGGCTGGAGTAGATTTTGAAAGTCAGCTCTGTGTGTGTGTGT 
               
               
                   
                 GTGTGTGTGTGTGTGAGAGCGTGTGTTTCTTTTAACGTTTTCAGC 
               
               
                   
                 CTACAGCATACAGGGTTCATGGTGGCAAGAAGATAACAAGATTTA 
               
               
                   
                 AATTATGGCCAGTGACTAGTGCTGCAAGAAGAACAACTACCTGCA 
               
               
                   
                 TTTAATGGGAAAGCAAAATCTCAGGCTTTGAGGGAAGTTAACATA 
               
               
                   
                 GGCTTGATTCTGGGTGGAAGCTGGGTGTGTAGTTATCTGGAGGCC 
               
               
                   
                 AGGCTGGAGCTCTCAGCTCACTATGGGTTCATCTTTATTGTCTCC 
               
               
                   
                 TTTCATCTCAACAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGG 
               
               
                   
                 ATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG 
               
               
                   
                 ATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTGTGCCCC 
               
               
                   
                 AGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATG 
               
               
                   
                 CGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTC 
               
               
                   
                 TTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACG 
               
               
                   
                 TAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATG 
               
               
                   
                 TGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTG 
               
               
                   
                 GTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAG 
               
               
                   
                 ATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCC 
               
               
                   
                 TCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGC 
               
               
                   
                 TCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTG 
               
               
                   
                 CTCACCATGGTGGCGAATTCGAAGCTTGAGCACGAGATCTGAGTC 
               
               
                   
                 CGGTAGGCCTAGCGGATCTGACGGTTCACTAAACCAGCTCTGCTT 
               
               
                   
                 ATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAAT 
               
               
                   
                 GGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGG 
               
               
                   
                 TGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGA 
               
               
                   
                 AATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGC 
               
               
                   
                 CAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATG 
               
               
                   
                 TACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAA 
               
               
                   
                 TGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTA 
               
               
                   
                 CTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTAC 
               
               
                   
                 CGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGC 
               
               
                   
                 GTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGG 
               
               
                   
                 TCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTA 
               
               
                   
                 ACGGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTT 
               
               
                   
                 CGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCT 
               
               
                   
                 GTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGA 
               
               
                   
                 CATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAAT 
               
               
                   
                 GCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGC 
               
               
                   
                 TTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAA 
               
               
                   
                 CAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGA 
               
               
                   
                 GGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCC 
               
               
                   
                 ATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGG 
               
               
                   
                 GTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTAC 
               
               
                   
                 CGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCT 
               
               
                   
                 TATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCC 
               
               
                   
                 AACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCC 
               
               
                   
                 TTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCA 
               
               
                   
                 AGACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGA 
               
               
                   
                 AGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACC 
               
               
                   
                 TTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTC 
               
               
                   
                 AGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAAC 
               
               
                   
                 TCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTT 
               
               
                   
                 TTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATT 
               
               
                   
                 CCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAA 
               
               
                   
                 AGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTT 
               
               
                   
                 CTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCAC 
               
               
                   
                 AACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAG 
               
               
                   
                 CGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTG 
               
               
                   
                 CCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGG 
               
               
                   
                 CCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTG 
               
               
                   
                 AAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG 
               
               
                   
                 ATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCA 
               
               
                   
                 TGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCT 
               
               
                   
                 GCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTA 
               
               
                   
                 CTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAG 
               
               
                   
                 AGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGG 
               
               
                   
                 CGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATG 
               
               
                   
                 CCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGAT 
               
               
                   
                 TCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACA 
               
               
                   
                 TAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAAT 
               
               
                   
                 GGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATT 
               
               
                   
                 CGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGT 
               
               
                   
                 ATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCC 
               
               
                   
                 CCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTG 
               
               
                   
                 TCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTA 
               
               
                   
                 GGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGG 
               
               
                   
                 GGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGG 
               
               
                   
                 GCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATC 
               
               
                   
                 GACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGT 
               
               
                   
                 TCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTT 
               
               
                   
                 CATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCG 
               
               
                   
                 TAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCC 
               
               
                   
                 TGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAA 
               
               
                   
                 CCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC 
               
               
                   
                 CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCT 
               
               
                   
                 GTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTC 
               
               
                   
                 ACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCT 
               
               
                   
                 GGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTT 
               
               
                   
                 ATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTT 
               
               
                   
                 ATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAG 
               
               
                   
                 GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA 
               
               
                   
                 CGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAA 
               
               
                   
                 GCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAA 
               
               
                   
                 ACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA 
               
               
                   
                 GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTT 
               
               
                   
                 TTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGG 
               
               
                   
                 GATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCT 
               
               
                   
                 TTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGA 
               
               
                   
                 GTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACC 
               
               
                   
                 TATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAA 
               
               
                   
                 ATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTT 
               
               
                   
                 TCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCA 
               
               
                   
                 AGTCTCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCC 
               
               
                   
                 TATCGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATC 
               
               
                   
                 ACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGA 
               
               
                   
                 GATCCGAGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAA 
               
               
                   
                 CGATCCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTGGC 
               
               
                   
                 AGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCG 
               
               
                   
                 TCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGT 
               
               
                   
                 TTAAACCTAGATATTGATAGTCTGATCGGTCAACGTATAATCGAG 
               
               
                   
                 TCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAG 
               
               
                   
                 GCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCT 
               
               
                   
                 TTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGC 
               
               
                   
                 TGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGG 
               
               
                   
                 GTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTT 
               
               
                   
                 TTCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTTTAAAGTTC 
               
               
                   
                 TGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGC 
               
               
                   
                 AACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGT 
               
               
                   
                 ATTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAA 
               
               
                   
                 GAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGG 
               
               
                   
                 CCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCG 
               
               
                   
                 CTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTT 
               
               
                   
                 GGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACA 
               
               
                   
                 CCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAA 
               
               
                   
                 CTGGCGAACTACTTACTCTAGCTTCCCGGCAACAGTTGATAGACT 
               
               
                   
                 GGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCC 
               
               
                   
                 TTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGC 
               
               
                   
                 GTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGC 
               
               
                   
                 CCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTA 
               
               
                   
                 TGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGA 
               
               
                   
                 TTAAGCATTGGTAACCGATTCTAGGTGCATTGGCGCAGAAAAAAA 
               
               
                   
                 TGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGA 
               
               
                   
                 TTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGT 
               
               
                   
                 GTCCTCCTTACCAGAAATTTATCCTTAAGATCGTTTAAACTCGAC 
               
               
                   
                 TCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGC 
               
               
                   
                 CGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATC 
               
               
                   
                 GTCAGCTTACCTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGG 
               
               
                   
                 ACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACA 
               
               
                   
                 GCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGT 
               
               
                   
                 TTAGAGGCCCCAAGGGGTTATGCTATCAATCGTTGCGTTACACAC 
               
               
                   
                 ACAAAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTAT 
               
               
                   
                 TATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTAC 
               
               
                   
                 GGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATA 
               
               
                   
                 ACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCG 
               
               
                   
                 CCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAAT 
               
               
                   
                 AGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAAC 
               
               
                   
                 TGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCC 
               
               
                   
                 CCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGC 
               
               
                   
                 CCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTA 
               
               
                   
                 CGTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTA 
               
               
                   
                 CATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAG 
               
               
                   
                 TCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAA 
               
               
                   
                 TCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGAC 
               
               
                   
                 GCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG 
               
               
                   
                 AGCTGGTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACC 
               
               
                   
                 ATCCACTCGACACACCCGCCAGCGGCCGC 
               
               
                   
                 (SEQID NO: 46) 
               
               
                   
                   
               
               
                   
                 LINE-1 plasmid ORF1-P2A-ORF2 GFP 
               
               
                   
                 (SEQ ID NO: 47) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGGGAAGCGGAGCTACTAACTTCAGCCTGCTGA 
               
               
                   
                 AGCAGGCTGGAGACGTGGAGGAGAACCCTGGACCTATGACCGGCT 
               
               
                   
                 CTAACTCACATATCACCATCCTTACACTTAACATTAACGGCCTCA 
               
               
                   
                 ACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCAC 
               
               
                   
                 AGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTA 
               
               
                   
                 GAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTTATC 
               
               
                   
                 AGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCT 
               
               
                   
                 CAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGG 
               
               
                   
                 AAGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAGAAC 
               
               
                   
                 TTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCT 
               
               
                   
                 TTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTC 
               
               
                   
                 ATACGTTGATTATGGGTGATTTCAATACACCATTGAGCACCCTGG 
               
               
                   
                 ATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCA 
               
               
                   
                 ATAGCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACTC 
               
               
                   
                 TTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCACATC 
               
               
                   
                 ATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGC 
               
               
                   
                 TGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAG 
               
               
                   
                 ATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGACCC 
               
               
                   
                 AGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACG 
               
               
                   
                 ATTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCT 
               
               
                   
                 TCGAAACAAATGAGAATAAGGATACTACCTATCAAAACCTTTGGG 
               
               
                   
                 ATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACG 
               
               
                   
                 CCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCT 
               
               
                   
                 CTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGG 
               
               
                   
                 CGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAG 
               
               
                   
                 AGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTA 
               
               
                   
                 GTTGGTTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGGCAC 
               
               
                   
                 GACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCA 
               
               
                   
                 AGAACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGA 
               
               
                   
                 CCACTATTCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTG 
               
               
                   
                 AGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGC 
               
               
                   
                 CACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTA 
               
               
                   
                 CAGGAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGA 
               
               
                   
                 AATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGT 
               
               
                   
                 ATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTA 
               
               
                   
                 TAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTA 
               
               
                   
                 TAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAA 
               
               
                   
                 ACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGA 
               
               
                   
                 ACAAGATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGA 
               
               
                   
                 TACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGT 
               
               
                   
                 TTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAG 
               
               
                   
                 CTAAGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGG 
               
               
                   
                 CATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACA 
               
               
                   
                 AACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTT 
               
               
                   
                 ACGATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCG 
               
               
                   
                 AGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCT 
               
               
                   
                 CCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTA 
               
               
                   
                 TTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAG 
               
               
                   
                 AGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGG 
               
               
                   
                 AGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTA 
               
               
                   
                 ACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTC 
               
               
                   
                 AGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATAA 
               
               
                   
                 TGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATC 
               
               
                   
                 TCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAA 
               
               
                   
                 ATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGT 
               
               
                   
                 GGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGA 
               
               
                   
                 AGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCC 
               
               
                   
                 CAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAA 
               
               
                   
                 CCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGT 
               
               
                   
                 CCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTG 
               
               
                   
                 ATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGT 
               
               
                   
                 ATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAAC 
               
               
                   
                 CATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCGATA 
               
               
                   
                 AGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACA 
               
               
                   
                 AGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGC 
               
               
                   
                 TCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTGGA 
               
               
                   
                 TCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAAG 
               
               
                   
                 AGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATT 
               
               
                   
                 TTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTG 
               
               
                   
                 ATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAAGG 
               
               
                   
                 AGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGA 
               
               
                   
                 TTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATAT 
               
               
                   
                 ATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATCCCA 
               
               
                   
                 TCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGG 
               
               
                   
                 ATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCT 
               
               
                   
                 TGGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATACCACC 
               
               
                   
                 TTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATA 
               
               
                   
                 GATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCT 
               
               
                   
                 GGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGC 
               
               
                   
                 GCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAA 
               
               
                   
                 TTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTT 
               
               
                   
                 ACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTACGA 
               
               
                   
                 TAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATT 
               
               
                   
                 GGATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGCAG 
               
               
                   
                 CAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGATGA 
               
               
                   
                 AGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGA 
               
               
                   
                 CAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAG 
               
               
                   
                 ACGATGACGACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGC 
               
               
                   
                 ACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATC 
               
               
                   
                 AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTA 
               
               
                   
                 ACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGC 
               
               
                   
                 CTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCT 
               
               
                   
                 CCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGC 
               
               
                   
                 CCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACG 
               
               
                   
                 CAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTT 
               
               
                   
                 CCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCA 
               
               
                   
                 TCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGG 
               
               
                   
                 GCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTC 
               
               
                   
                 CATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGT 
               
               
                   
                 CCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTT 
               
               
                   
                 CCCGCGAACAAACGACCCAACACCCGTGCGTTTTATTCTGTCTTT 
               
               
                   
                 TTATTGCCGATCCCCTCAGAAGAACTCGTCAAGAAGGCGATAGAA 
               
               
                   
                 GGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAG 
               
               
                   
                 GAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACG 
               
               
                   
                 GGTAGCCAACGCTATGTCCTGATAGCGGTCGGCCGCTTTACTTGT 
               
               
                   
                 ACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACT 
               
               
                   
                 CCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCA 
               
               
                   
                 GGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGG 
               
               
                   
                 GGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCCAGGT 
               
               
                   
                 GAGTCCAGGAGATGTTTCAGCACTGTTGCCTTTAGTCTCGAGGCA 
               
               
                   
                 ACTTAGACAACTGAGTATTGATCTGAGCACAGCAGGGTGTGAGCT 
               
               
                   
                 GTTTGAAGATACTGGGGTTGGGGGTGAAGAAACTGCAGAGGACTA 
               
               
                   
                 ACTGGGCTGAGACCCAGTGGCAATGTTTTAGGGCCTAAGGAATGC 
               
               
                   
                 CTCTGAAAATCTAGATGGACAACTTTGACTTTGAGAAAAGAGAGG 
               
               
                   
                 TGGAAATGAGGAAAATGACTTTTCTTTATTAGATTTCGGTAGAAA 
               
               
                   
                 GAACTTTCATCTTTCCCCTATTTTTGTTATTCGTTTTAAAACATC 
               
               
                   
                 TATCTGGAGGCAGGACAAGTATGGTCATTAAAAAGATGCAGGCAG 
               
               
                   
                 AAGGCATATATTGGCTCAGTCAAAGTGGGGAACTTTGGTGGCCAA 
               
               
                   
                 ACATACATTGCTAAGGCTATTCCTATATCAGCTGGACACATATAA 
               
               
                   
                 AATGCTGCTAATGCTTCATTACAAACTTATATCCTTTAATTCCAG 
               
               
                   
                 ATGGGGGCAAAGTATGTCCAGGGGTGAGGAACAATTGAAACATTT 
               
               
                   
                 GGGCTGGAGTAGATTTTGAAAGTCAGCTCTGTGTGTGTGTGTGTG 
               
               
                   
                 TGTGTGTGTGTGAGAGCGTGTGTTTCTTTTAACGTTTTCAGCCTA 
               
               
                   
                 CAGCATACAGGGTTCATGGTGGCAAGAAGATAACAAGATTTAAAT 
               
               
                   
                 TATGGCCAGTGACTAGTGCTGCAAGAAGAACAACTACCTGCATTT 
               
               
                   
                 AATGGGAAAGCAAAATCTCAGGCTTTGAGGGAAGTTAACATAGGC 
               
               
                   
                 TTGATTCTGGGTGGAAGCTGGGTGTGTAGTTATCTGGAGGCCAGG 
               
               
                   
                 CTGGAGCTCTCAGCTCACTATGGGTTCATCTTTATTGTCTCCTTT 
               
               
                   
                 CATCTCAACAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATC 
               
               
                   
                 TTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATA 
               
               
                   
                 TAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTGTGCCCCAGG 
               
               
                   
                 ATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGG 
               
               
                   
                 TTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTG 
               
               
                   
                 TAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAG 
               
               
                   
                 CCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGG 
               
               
                   
                 TCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTC 
               
               
                   
                 ACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATG 
               
               
                   
                 AACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCTCG 
               
               
                   
                 CCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCG 
               
               
                   
                 ACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTC 
               
               
                   
                 ACCATGGTGGCGAATTCGAAGCTTGAGCACGAGATCTGAGTCCGG 
               
               
                   
                 TAGGCCTAGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATA 
               
               
                   
                 TAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGG 
               
               
                   
                 GCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGC 
               
               
                   
                 CAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAAT 
               
               
                   
                 CCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAA 
               
               
                   
                 AACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTAC 
               
               
                   
                 TGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGC 
               
               
                   
                 CAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTT 
               
               
                   
                 GGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGT 
               
               
                   
                 AAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTT 
               
               
                   
                 ACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCG 
               
               
                   
                 TTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACG 
               
               
                   
                 GGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGC 
               
               
                   
                 CCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTC 
               
               
                   
                 TAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACAT 
               
               
                   
                 GATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCA 
               
               
                   
                 GTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTT 
               
               
                   
                 ATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAA 
               
               
                   
                 TTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGT 
               
               
                   
                 TTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATC 
               
               
                   
                 TCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTC 
               
               
                   
                 TTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGG 
               
               
                   
                 CATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTAT 
               
               
                   
                 ACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAAC 
               
               
                   
                 TTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTA 
               
               
                   
                 AGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGA 
               
               
                   
                 CATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGT 
               
               
                   
                 AGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTG 
               
               
                   
                 GGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGC 
               
               
                   
                 AACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCC 
               
               
                   
                 GCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTT 
               
               
                   
                 TATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCA 
               
               
                   
                 GAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGG 
               
               
                   
                 AGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCTC 
               
               
                   
                 CCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAAC 
               
               
                   
                 AGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGC 
               
               
                   
                 AGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCC 
               
               
                   
                 TGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCA 
               
               
                   
                 CGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAG 
               
               
                   
                 CGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATC 
               
               
                   
                 TCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGG 
               
               
                   
                 CTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCC 
               
               
                   
                 CATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTC 
               
               
                   
                 GGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGC 
               
               
                   
                 ATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGC 
               
               
                   
                 GGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCT 
               
               
                   
                 GCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCA 
               
               
                   
                 TCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAG 
               
               
                   
                 CGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGG 
               
               
                   
                 CTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGC 
               
               
                   
                 AGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATG 
               
               
                   
                 TAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCT 
               
               
                   
                 CCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCC 
               
               
                   
                 TTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGT 
               
               
                   
                 GTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGG 
               
               
                   
                 AGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCT 
               
               
                   
                 CTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGAC 
               
               
                   
                 TTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCG 
               
               
                   
                 ATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCAT 
               
               
                   
                 TTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA 
               
               
                   
                 AAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGA 
               
               
                   
                 CGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCC 
               
               
                   
                 GACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCT 
               
               
                   
                 CGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTC 
               
               
                   
                 CGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACG 
               
               
                   
                 CTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGG 
               
               
                   
                 CTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC 
               
               
                   
                 CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATC 
               
               
                   
                 GCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTA 
               
               
                   
                 TGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGG 
               
               
                   
                 CTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCC 
               
               
                   
                 AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACA 
               
               
                   
                 AACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGAT 
               
               
                   
                 TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTC 
               
               
                   
                 TACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT 
               
               
                   
                 TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTT 
               
               
                   
                 AAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTA 
               
               
                   
                 AACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTAT 
               
               
                   
                 CTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATT 
               
               
                   
                 TCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCG 
               
               
                   
                 TCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGT 
               
               
                   
                 CTCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTAT 
               
               
                   
                 CGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATCACC 
               
               
                   
                 CGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGAT 
               
               
                   
                 CCGAGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGA 
               
               
                   
                 TCCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTGGCAGT 
               
               
                   
                 CAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCA 
               
               
                   
                 GATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTA 
               
               
                   
                 AACCTAGATATTGATAGTCTGATCGGTCAACGTATAATCGAGTCC 
               
               
                   
                 TAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCT 
               
               
                   
                 TTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTT 
               
               
                   
                 TTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGG 
               
               
                   
                 TGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTT 
               
               
                   
                 ACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTC 
               
               
                   
                 GCCCCGAAGAACGCTTTCCAATGATGAGCACTTTTAAAGTTCTGC 
               
               
                   
                 TATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAAC 
               
               
                   
                 TCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATT 
               
               
                   
                 CACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAG 
               
               
                   
                 AATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA 
               
               
                   
                 ACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTT 
               
               
                   
                 TTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGG 
               
               
                   
                 AACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCA 
               
               
                   
                 CGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTG 
               
               
                   
                 GCGAACTACTTACTCTAGCTTCCCGGCAACAGTTGATAGACTGGA 
               
               
                   
                 TGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTC 
               
               
                   
                 CGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTG 
               
               
                   
                 GGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCT 
               
               
                   
                 CCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGG 
               
               
                   
                 ATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA 
               
               
                   
                 AGCATTGGTAACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGC 
               
               
                   
                 CTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTC 
               
               
                   
                 AGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTC 
               
               
                   
                 CTCCTTACCAGAAATTTATCCTTAAGATCGTTTAAACTCGACTCT 
               
               
                   
                 GGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGT 
               
               
                   
                 GGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTC 
               
               
                   
                 AGCTTACCTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACC 
               
               
                   
                 ATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCA 
               
               
                   
                 GCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTA 
               
               
                   
                 GAGGCCCCAAGGGGTTATGCTATCAATCGTTGCGTTACACACACA 
               
               
                   
                 AAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTATTAT 
               
               
                   
                 CTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGG 
               
               
                   
                 GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACT 
               
               
                   
                 TACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCC 
               
               
                   
                 ATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGG 
               
               
                   
                 GACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCC 
               
               
                   
                 TATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCA 
               
               
                   
                 GTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGT 
               
               
                   
                 ATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTACAT 
               
               
                   
                 CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCT 
               
               
                   
                 CCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCA 
               
               
                   
                 ACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCA 
               
               
                   
                 AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC 
               
               
                   
                 TGGTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATC 
               
               
                   
                 CACTCGACACACCCGCCAGCGGCCGC 
               
               
                   
                 (SEQ ID NO: 47) 
               
               
                   
                   
               
               
                   
                 LINE-1 plasmid ORF1-T2A ORF2 GFP 
               
               
                   
                 (SEQ ID NO: 48) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGGGAAGCGGAGAGGGCAGAGGAAGTCTGCTAA 
               
               
                   
                 CATGCGGTGACGTCGAGGAGAATCCTGGACCTATGACCGGCTCTA 
               
               
                   
                 ACTCACATATCACCATCCTTACACTTAACATTAACGGCCTCAACT 
               
               
                   
                 CAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGG 
               
               
                   
                 ATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAG 
               
               
                   
                 ATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTTATCAGG 
               
               
                   
                 CGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAG 
               
               
                   
                 ACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAG 
               
               
                   
                 GTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAGAACTTA 
               
               
                   
                 CCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTA 
               
               
                   
                 TCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATA 
               
               
                   
                 CGTTGATTATGGGTGATTTCAATACACCATTGAGCACCCTGGATC 
               
               
                   
                 GCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATA 
               
               
                   
                 GCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTC 
               
               
                   
                 ATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCACATCATA 
               
               
                   
                 CATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGT 
               
               
                   
                 CAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATC 
               
               
                   
                 ATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGA 
               
               
                   
                 GCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATT 
               
               
                   
                 ATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCG 
               
               
                   
                 AAACAAATGAGAATAAGGATACTACCTATCAAAACCTTTGGGATG 
               
               
                   
                 CCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCT 
               
               
                   
                 ATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTC 
               
               
                   
                 AGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGT 
               
               
                   
                 CAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGA 
               
               
                   
                 TCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTT 
               
               
                   
                 GGTTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGAC 
               
               
                   
                 TGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGA 
               
               
                   
                 ACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCA 
               
               
                   
                 CTATTCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGA 
               
               
                   
                 ACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCAC 
               
               
                   
                 GGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAG 
               
               
                   
                 GAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAAT 
               
               
                   
                 CTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATA 
               
               
                   
                 TGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAG 
               
               
                   
                 AAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAA 
               
               
                   
                 TACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACT 
               
               
                   
                 TCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACA 
               
               
                   
                 AGATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATAC 
               
               
                   
                 ATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTA 
               
               
                   
                 ACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTA 
               
               
                   
                 AGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCAT 
               
               
                   
                 TCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAAAC 
               
               
                   
                 TCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACG 
               
               
                   
                 ATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGG 
               
               
                   
                 CCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTCCC 
               
               
                   
                 CGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTC 
               
               
                   
                 GTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAGG 
               
               
                   
                 TCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGGAGA 
               
               
                   
                 ATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACT 
               
               
                   
                 TTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCAGG 
               
               
                   
                 CCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATAATGG 
               
               
                   
                 GTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCG 
               
               
                   
                 GAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAAATT 
               
               
                   
                 ACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGTGGA 
               
               
                   
                 AGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGA 
               
               
                   
                 TGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCCCAA 
               
               
                   
                 TTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAACCC 
               
               
                   
                 TTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCA 
               
               
                   
                 TCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTGATT 
               
               
                   
                 TTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGTATT 
               
               
                   
                 GGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCAT 
               
               
                   
                 CAGAGATAATGCCCCACATCTATAATTACCTTATATTCGATAAGC 
               
               
                   
                 CAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACAAGT 
               
               
                   
                 GGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCG 
               
               
                   
                 ACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTGGATCA 
               
               
                   
                 AGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAAGAGA 
               
               
                   
                 ATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTA 
               
               
                   
                 TGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTGATA 
               
               
                   
                 AGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAAGGAGA 
               
               
                   
                 CCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTT 
               
               
                   
                 TCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATATATA 
               
               
                   
                 ACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATCCCATCA 
               
               
                   
                 AGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATA 
               
               
                   
                 TCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGG 
               
               
                   
                 CCATTCGTGAGATGCAGATTAAGACGACCATGCGATACCACCTTA 
               
               
                   
                 CCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGAT 
               
               
                   
                 GTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGT 
               
               
                   
                 GGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCT 
               
               
                   
                 TTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTC 
               
               
                   
                 CCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACA 
               
               
                   
                 AGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTACGATAG 
               
               
                   
                 CTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGA 
               
               
                   
                 TCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAA 
               
               
                   
                 TTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGC 
               
               
                   
                 TGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAA 
               
               
                   
                 AGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACG 
               
               
                   
                 ATGACGACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACT 
               
               
                   
                 GACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAAC 
               
               
                   
                 CTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACT 
               
               
                   
                 ATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTT 
               
               
                   
                 TGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCT 
               
               
                   
                 TGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCG 
               
               
                   
                 TTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAA 
               
               
                   
                 CCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCG 
               
               
                   
                 GGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCG 
               
               
                   
                 CCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCA 
               
               
                   
                 CTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCAT 
               
               
                   
                 GGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCT 
               
               
                   
                 TCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCC 
               
               
                   
                 GCGAACAAACGACCCAACACCCGTGCGTTTTATTCTGTCTTTTTA 
               
               
                   
                 TTGCCGATCCCCTCAGAAGAACTCGTCAAGAAGGCGATAGAAGGC 
               
               
                   
                 GATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAGGAA 
               
               
                   
                 GCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGGGT 
               
               
                   
                 AGCCAACGCTATGTCCTGATAGCGGTCGGCCGCTTTACTTGTACA 
               
               
                   
                 GCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCA 
               
               
                   
                 GCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGGG 
               
               
                   
                 CGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGC 
               
               
                   
                 CGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCCAGGTGAG 
               
               
                   
                 TCCAGGAGATGTTTCAGCACTGTTGCCTTTAGTCTCGAGGCAACT 
               
               
                   
                 TAGACAACTGAGTATTGATCTGAGCACAGCAGGGTGTGAGCTGTT 
               
               
                   
                 TGAAGATACTGGGGTTGGGGGTGAAGAAACTGCAGAGGACTAACT 
               
               
                   
                 GGGCTGAGACCCAGTGGCAATGTTTTAGGGCCTAAGGAATGCCTC 
               
               
                   
                 TGAAAATCTAGATGGACAACTTTGACTTTGAGAAAAGAGAGGTGG 
               
               
                   
                 AAATGAGGAAAATGACTTTTCTTTATTAGATTTCGGTAGAAAGAA 
               
               
                   
                 CTTTCATCTTTCCCCTATTTTTGTTATTCGTTTTAAAACATCTAT 
               
               
                   
                 CTGGAGGCAGGACAAGTATGGTCATTAAAAAGATGCAGGCAGAAG 
               
               
                   
                 GCATATATTGGCTCAGTCAAAGTGGGGAACTTTGGTGGCCAAACA 
               
               
                   
                 TACATTGCTAAGGCTATTCCTATATCAGCTGGACACATATAAAAT 
               
               
                   
                 GCTGCTAATGCTTCATTACAAACTTATATCCTTTAATTCCAGATG 
               
               
                   
                 GGGGCAAAGTATGTCCAGGGGTGAGGAACAATTGAAACATTTGGG 
               
               
                   
                 CTGGAGTAGATTTTGAAAGTCAGCTCTGTGTGTGTGTGTGTGTGT 
               
               
                   
                 GTGTGTGTGAGAGCGTGTGTTTCTTTTAACGTTTTCAGCCTACAG 
               
               
                   
                 CATACAGGGTTCATGGTGGCAAGAAGATAACAAGATTTAAATTAT 
               
               
                   
                 GGCCAGTGACTAGTGCTGCAAGAAGAACAACTACCTGCATTTAAT 
               
               
                   
                 GGGAAAGCAAAATCTCAGGCTTTGAGGGAAGTTAACATAGGCTTG 
               
               
                   
                 ATTCTGGGTGGAAGCTGGGTGTGTAGTTATCTGGAGGCCAGGCTG 
               
               
                   
                 GAGCTCTCAGCTCACTATGGGTTCATCTTTATTGTCTCCTTTCAT 
               
               
                   
                 CTCAACAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTG 
               
               
                   
                 AAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAG 
               
               
                   
                 ACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTGTGCCCCAGGATG 
               
               
                   
                 TTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTC 
               
               
                   
                 ACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAG 
               
               
                   
                 TTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGCCT 
               
               
                   
                 TCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCG 
               
               
                   
                 GGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACG 
               
               
                   
                 AGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAAC 
               
               
                   
                 TTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCTCGCCG 
               
               
                   
                 GACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACC 
               
               
                   
                 AGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACC 
               
               
                   
                 ATGGTGGCGAATTCGAAGCTTGAGCACGAGATCTGAGTCCGGTAG 
               
               
                   
                 GCCTAGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAG 
               
               
                   
                 ACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCG 
               
               
                   
                 GAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAA 
               
               
                   
                 AACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCC 
               
               
                   
                 CGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAAC 
               
               
                   
                 CGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGC 
               
               
                   
                 CAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAG 
               
               
                   
                 GCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGC 
               
               
                   
                 ATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAA 
               
               
                   
                 TACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACT 
               
               
                   
                 ATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTG 
               
               
                   
                 GGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGGGC 
               
               
                   
                 CTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCT 
               
               
                   
                 CAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAG 
               
               
                   
                 CTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGAT 
               
               
                   
                 AAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTG 
               
               
                   
                 AAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATT 
               
               
                   
                 TGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTG 
               
               
                   
                 CATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTT 
               
               
                   
                 TTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCT 
               
               
                   
                 ATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTG 
               
               
                   
                 AGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCAT 
               
               
                   
                 TGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACT 
               
               
                   
                 CCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTG 
               
               
                   
                 CCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGG 
               
               
                   
                 TCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACAT 
               
               
                   
                 TCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGT 
               
               
                   
                 TCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGC 
               
               
                   
                 TATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAAC 
               
               
                   
                 CATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCC 
               
               
                   
                 CAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTAT 
               
               
                   
                 TTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAA 
               
               
                   
                 GTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGG 
               
               
                   
                 TAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCG 
               
               
                   
                 CCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGA 
               
               
                   
                 CAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGG 
               
               
                   
                 GGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGA 
               
               
                   
                 ATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGA 
               
               
                   
                 CGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGG 
               
               
                   
                 GAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCC 
               
               
                   
                 TGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTG 
               
               
                   
                 ATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCAT 
               
               
                   
                 TCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGA 
               
               
                   
                 TGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATC 
               
               
                   
                 AGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGA 
               
               
                   
                 TGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCT 
               
               
                   
                 TGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCG 
               
               
                   
                 ACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGT 
               
               
                   
                 TGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTG 
               
               
                   
                 ACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGC 
               
               
                   
                 GCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAA 
               
               
                   
                 GCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCC 
               
               
                   
                 CCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTT 
               
               
                   
                 CCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTC 
               
               
                   
                 ATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGG 
               
               
                   
                 ATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTA 
               
               
                   
                 TGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTG 
               
               
                   
                 TCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATC 
               
               
                   
                 TGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTA 
               
               
                   
                 AATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAA 
               
               
                   
                 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGA 
               
               
                   
                 GCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGAC 
               
               
                   
                 AGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGT 
               
               
                   
                 GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 
               
               
                   
                 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTG 
               
               
                   
                 TAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTG 
               
               
                   
                 TGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGG 
               
               
                   
                 TAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCC 
               
               
                   
                 ACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGT 
               
               
                   
                 AGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA 
               
               
                   
                 CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGT 
               
               
                   
                 TACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAAC 
               
               
                   
                 CACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTAC 
               
               
                   
                 GCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTAC 
               
               
                   
                 GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTT 
               
               
                   
                 GGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAA 
               
               
                   
                 TTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAAC 
               
               
                   
                 TTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTC 
               
               
                   
                 AGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCC 
               
               
                   
                 GAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCGTCC 
               
               
                   
                 GATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTC 
               
               
                   
                 TTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGC 
               
               
                   
                 CAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATCACCCGA 
               
               
                   
                 GAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCG 
               
               
                   
                 AGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCC 
               
               
                   
                 TCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTGGCAGTCAG 
               
               
                   
                 CCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGAT 
               
               
                   
                 ATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAAC 
               
               
                   
                 CTAGATATTGATAGTCTGATCGGTCAACGTATAATCGAGTCCTAG 
               
               
                   
                 CTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTC 
               
               
                   
                 GCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTG 
               
               
                   
                 CGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGA 
               
               
                   
                 AAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACA 
               
               
                   
                 TCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCC 
               
               
                   
                 CCGAAGAACGCTTTCCAATGATGAGCACTTTTAAAGTTCTGCTAT 
               
               
                   
                 GTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCG 
               
               
                   
                 GTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCAC 
               
               
                   
                 CAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAAT 
               
               
                   
                 TATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACT 
               
               
                   
                 TACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTT 
               
               
                   
                 TGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAAC 
               
               
                   
                 CGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA 
               
               
                   
                 TGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCG 
               
               
                   
                 AACTACTTACTCTAGCTTCCCGGCAACAGTTGATAGACTGGATGG 
               
               
                   
                 AGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGG 
               
               
                   
                 CTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGT 
               
               
                   
                 CTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCC 
               
               
                   
                 GTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATG 
               
               
                   
                 AACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGC 
               
               
                   
                 ATTGGTAACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTG 
               
               
                   
                 ATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGC 
               
               
                   
                 AACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTC 
               
               
                   
                 CTTACCAGAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGGC 
               
               
                   
                 TCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGTGGC 
               
               
                   
                 GCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGC 
               
               
                   
                 TTACCTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCATT 
               
               
                   
                 AGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCT 
               
               
                   
                 TCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAG 
               
               
                   
                 GCCCCAAGGGGTTATGCTATCAATCGTTGCGTTACACACACAAAA 
               
               
                   
                 AACCAACACACATCCATCTTCGATGGATAGCGATTTTATTATCTA 
               
               
                   
                 ACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTC 
               
               
                   
                 ATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTAC 
               
               
                   
                 GGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATT 
               
               
                   
                 GACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGAC 
               
               
                   
                 TTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCA 
               
               
                   
                 CTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTAT 
               
               
                   
                 TGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTA 
               
               
                   
                 CATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATT 
               
               
                   
                 AGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTACATCAA 
               
               
                   
                 TGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCA 
               
               
                   
                 CCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACG 
               
               
                   
                 GGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAAT 
               
               
                   
                 GGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGG 
               
               
                   
                 TTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCAC 
               
               
                   
                 TCGACACACCCGCCAGCGGCCGC 
               
               
                   
                 (SEQ ID NO: 48) 
               
               
                   
                   
               
               
                   
                 LINE-1 ORF2-MCP MS2 mRNA (SEQ ID NO: 49) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGA 
               
               
                   
                 AGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATA 
               
               
                   
                 GTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTT 
               
               
                   
                 AACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCC 
               
               
                   
                 AGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAG 
               
               
                   
                 ACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGA 
               
               
                   
                 TGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGA 
               
               
                   
                 GTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAA 
               
               
                   
                 ATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGC 
               
               
                   
                 ATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAAC 
               
               
                   
                 ACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAG 
               
               
                   
                 CGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACA 
               
               
                   
                 CCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAA 
               
               
                   
                 GACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATT 
               
               
                   
                 GATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTC 
               
               
                   
                 TTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTC 
               
               
                   
                 GGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATT 
               
               
                   
                 ACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGA 
               
               
                   
                 ATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAAT 
               
               
                   
                 AACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCA 
               
               
                   
                 GAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACC 
               
               
                   
                 TATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAG 
               
               
                   
                 TTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAG 
               
               
                   
                 ATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAA 
               
               
                   
                 CAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATT 
               
               
                   
                 CGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAA 
               
               
                   
                 ATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATA 
               
               
                   
                 GACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAAC 
               
               
                   
                 CAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGAC 
               
               
                   
                 CCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTG 
               
               
                   
                 TATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTG 
               
               
                   
                 GATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCC 
               
               
                   
                 CTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAAC 
               
               
                   
                 TCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCT 
               
               
                   
                 GAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTC 
               
               
                   
                 AAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCC 
               
               
                   
                 TTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGAT 
               
               
                   
                 ACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATC 
               
               
                   
                 GACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAA 
               
               
                   
                 CATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCT 
               
               
                   
                 GGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATT 
               
               
                   
                 CAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCT 
               
               
                   
                 ATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATG 
               
               
                   
                 CTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAG 
               
               
                   
                 ATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTT 
               
               
                   
                 AACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGC 
               
               
                   
                 CAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAG 
               
               
                   
                 GTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATA 
               
               
                   
                 CAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGAT 
               
               
                   
                 ATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTT 
               
               
                   
                 CTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATT 
               
               
                   
                 AACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAG 
               
               
                   
                 ACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGC 
               
               
                   
                 AAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAA 
               
               
                   
                 GATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAG 
               
               
                   
                 GAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGC 
               
               
                   
                 AGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATAT 
               
               
                   
                 CGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACG 
               
               
                   
                 GAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGA 
               
               
                   
                 GCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGT 
               
               
                   
                 GGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTA 
               
               
                   
                 ACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAG 
               
               
                   
                 TGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAAT 
               
               
                   
                 TACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAA 
               
               
                   
                 GACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATA 
               
               
                   
                 TGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAA 
               
               
                   
                 ATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACT 
               
               
                   
                 ATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATA 
               
               
                   
                 GGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCC 
               
               
                   
                 ACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGC 
               
               
                   
                 TTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCC 
               
               
                   
                 ACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGG 
               
               
                   
                 TTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAG 
               
               
                   
                 AAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGG 
               
               
                   
                 CATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAG 
               
               
                   
                 AAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACG 
               
               
                   
                 ACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAG 
               
               
                   
                 AAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGC 
               
               
                   
                 ACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTT 
               
               
                   
                 TGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATT 
               
               
                   
                 CCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAA 
               
               
                   
                 TACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATC 
               
               
                   
                 GCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGC 
               
               
                   
                 CCCACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACC 
               
               
                   
                 ATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTC 
               
               
                   
                 GTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTG 
               
               
                   
                 TCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGT 
               
               
                   
                 GGTAACGCTTCTAACTTTACTCAGTTCGTTCTCGTCGACAATGGC 
               
               
                   
                 GGAACTGGCGACGTGACTGTCGCCCCAAGCAACTTCGCTAACGGG 
               
               
                   
                 ATCGCTGAATGGATCAGCTCTAACTCGCGTTCACAGGCTTACAAA 
               
               
                   
                 GTAACCTGTAGCGTTCGTCAGAGCTCTGCGCAGAATCGCAAATAC 
               
               
                   
                 ACCATCAAAGTCGAGGTGCCTAAAGGCGCCTGGCGTTCGTACTTA 
               
               
                   
                 AATATGGAACTAACCATTCCAATTTTCGCCACGAATTCCGACTGC 
               
               
                   
                 GAGCTTATTGTTAAGGCAATGCAAGGTCTCCTAAAAGATGGAAAC 
               
               
                   
                 CCGATTCCCTCAGCAATCGCAGCAAACTCCGGCATCTACGCCATG 
               
               
                   
                 GCCAGCAACTTCACCCAGTTCGTGCTGGTGGACAACGGCGGCACC 
               
               
                   
                 GGCGACGTGACCGTGGCCCCCAGCAACTTCGCCAACGGCATCGCC 
               
               
                   
                 GAGTGGATCAGCAGCAACAGCAGAAGCCAGGCCTACAAGGTGACC 
               
               
                   
                 TGCAGCGTGAGACAGAGCAGCGCCCAGAACAGAAAGTACACCATC 
               
               
                   
                 AAGGTGGAGGTGCCCAAGGGCGCCTGGAGAAGCTACCTGAACATG 
               
               
                   
                 GAGCTGACCATCCCCATCTTCGCCACCAACAGCGACTGCGAGCTG 
               
               
                   
                 ATCGTGAAGGCCATGCAGGGCCTGCTGAAGGACGGCAACCCCATC 
               
               
                   
                 CCCAGCGCCATCGCCGCCAACAGCGGCATCTACGACTACAAAGAC 
               
               
                   
                 GATGACGACAAGTAAAGCAACCTACAAACGGGTGGAGGATCACCC 
               
               
                   
                 CACCCGACACTTCACAATCAAGGGGTACAATACACAAGGGTGGAG 
               
               
                   
                 GAACACCCCACCCTCCAGACACATTACACAGAAATCCAATCAAAC 
               
               
                   
                 AGAAGCACCATCAGGGCTTCTGCTACCAAATTTATCTCAAAAAAC 
               
               
                   
                 TACAACAAGGAATCACCATCAGGGATTCCCTGTGCAATATACGTC 
               
               
                   
                 AAACGAGGGCCACGACGGGAGGACGATCACGCCTCCCGAATATCG 
               
               
                   
                 GCATGTCTGGCTTTCGAATTCAGTGCGTGGAGCATCAGCCCACGC 
               
               
                   
                 AGCCAATCAGAGTCGAATACAAGTCGACTTTCGCGAAGAGCATCA 
               
               
                   
                 GCCTTCGCGCCATTCTTACACAAACCACACTCTCCCCTACAGGAA 
               
               
                   
                 CAGCATCAGCGTTCCTGCCCAGTACCCAACTCAAGAAAATTTATG 
               
               
                   
                 TCCCCATGCAGCATCAGCGCATGGGCCCCAAGAATACATCCCCAA 
               
               
                   
                 CAAAATCACATCCGAGCACCAACAGGGCTCGGAGTGTTGTTTCTT 
               
               
                   
                 GTCCAACTGGACAAACCCTCCATGGACCATCAGGCCATGGACTCT 
               
               
                   
                 CACCAACAAGACAAAAACTACTCTTCTCGAAGCAGCATCAGCGCT 
               
               
                   
                 TCGAAACACTCGAGCATACATTGTGCCTATTTCTTGGGTGGACGA 
               
               
                   
                 TCACGCCACCCATGCTCTCACGAATTTCAAAACACGGACAAGGAC 
               
               
                   
                 GAGCACCACCAGGGCTCGTCGTTCCACGTCCAATACGATTACTTA 
               
               
                   
                 CCTTTCGGGATCACGATCACGGATCCCGCAGCTACATCACTTCCA 
               
               
                   
                 CTCAGGACATTCAAGCATGCACGATCACGGCATGCTCCACAAGTC 
               
               
                   
                 TCAACCACAGAAACTACCAAATGGGTTCAGCACCAGCGAACCCAC 
               
               
                   
                 TCCTACCTCAAACCTCTTCCCACAAAACTGGCAAGCAGGATCACC 
               
               
                   
                 GCTTGCCCATTCCAACATACCAAATCAAAAACAATTACTGGTACA 
               
               
                   
                 GCATCAGCGTACCAGCCCACATCTCTCACTACTATCAAAAACCAA 
               
               
                   
                 ACCGTTCAGCAACAGCGAACGGTACACACGGAAAAATCAACTGGT 
               
               
                   
                 TTACAAATACGAAAGACGATCACGCTTTCGTCCAGCGCAAACTAT 
               
               
                   
                 TACGAAAAACATCCGACGGGAAGAGCAACAGCCTTCCCGCGGCGG 
               
               
                   
                 AAAACCTCACAAAAACACGACAAACGGATGCACGAACACGGCATC 
               
               
                   
                 CGCCGACAACCCACAAACTTACAACCAGGCAAACGGTGCAGGATC 
               
               
                   
                 ACCGCACCGTACATCAAACACCTCAGATCTCATGCTTCTAGAAGT 
               
               
                   
                 TGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATC 
               
               
                   
                 CGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTG 
               
               
                   
                 ACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATAC 
               
               
                   
                 GCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCT 
               
               
                   
                 TTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTAT 
               
               
                   
                 GAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACT 
               
               
                   
                 GTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACC 
               
               
                   
                 TGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCC 
               
               
                   
                 ACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGG 
               
               
                   
                 GCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAG 
               
               
                   
                 CTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATT 
               
               
                   
                 CTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCA 
               
               
                   
                 GCGGACCTTCCTTCCCGCTGAGAGACACAAAAAATTCCAACACAC 
               
               
                   
                 TATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATG 
               
               
                   
                 CTCAAGGGGCTTCATGATGTCCCCATAATTTTTGGCAGAGGGAAA 
               
               
                   
                 AAGATCTCAGTGGTATTTGTGAGCCAGGGCATTGGCCTTCTGATA 
               
               
                   
                 GGCAGCCTGCACCTGAGGAGTGCGGCCGCTTTACTTGTACAGCTC 
               
               
                   
                 GTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAG 
               
               
                   
                 GACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGGGCGGA 
               
               
                   
                 CTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTC 
               
               
                   
                 GCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCT 
               
               
                   
                 GCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTCACCTTGATGCC 
               
               
                   
                 GTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTA 
               
               
                   
                 GTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAA 
               
               
                   
                 GTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCCCTC 
               
               
                   
                 GAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAA 
               
               
                   
                 GAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTT 
               
               
                   
                 GAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCA 
               
               
                   
                 CTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGGGCAC 
               
               
                   
                 GGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCC 
               
               
                   
                 GTAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTG 
               
               
                   
                 GCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCACCCC 
               
               
                   
                 GGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGGGATCTGA 
               
               
                   
                 CGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACAT 
               
               
                   
                 TTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTG 
               
               
                   
                 ACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGC 
               
               
                   
                 TATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTA 
               
               
                   
                 ATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC 
               
               
                   
                 CATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCG 
               
               
                   
                 TCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGA 
               
               
                   
                 TGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGA 
               
               
                   
                 CGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTC 
               
               
                   
                 ATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCAGGC 
               
               
                   
                 GGGCCATTTACCGTAAGTTATGTAACGGGCCTGCTGCCGGCTCTG 
               
               
                   
                 CGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATC 
               
               
                   
                 TCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGA 
               
               
                   
                 TACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGATGA 
               
               
                   
                 GTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTAT 
               
               
                   
                 TTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAG 
               
               
                   
                 CTGCAATAAACAAGTT 
               
               
                   
                 (SEQ ID NO: 49) 
               
               
                   
                   
               
               
                   
                 LINEI ORF2-minke mRNA GFP (SEQ ID NO: 50) 
               
               
                   
                 TAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCC 
               
               
                   
                 CACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCT 
               
               
                   
                 GGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGC 
               
               
                   
                 GATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGA 
               
               
                   
                 AGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCC 
               
               
                   
                 GTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTA 
               
               
                   
                 AGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGAT 
               
               
                   
                 CCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACG 
               
               
                   
                 AGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCA 
               
               
                   
                 AGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAG 
               
               
                   
                 AAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAG 
               
               
                   
                 AAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAG 
               
               
                   
                 AGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGA 
               
               
                   
                 AGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGG 
               
               
                   
                 GCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCC 
               
               
                   
                 AGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGA 
               
               
                   
                 AGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTA 
               
               
                   
                 TCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGC 
               
               
                   
                 GAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAG 
               
               
                   
                 AGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGA 
               
               
                   
                 ACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGA 
               
               
                   
                 AGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATA 
               
               
                   
                 GTATACATGGTCATAGGAACTTACATTTCGATTATTACCTTAAAC 
               
               
                   
                 GTGAATGGGTTAAATGCCCCAACCAAGAGACATCGGCTGGCTGAA 
               
               
                   
                 TGGATTCAGAAACAGGACCCCTATATTTGCTGTCTGCAGGAGACC 
               
               
                   
                 CACTTCCGTCCTCGCGACACATACAGACTGAAAGTGAGGGGCTGG 
               
               
                   
                 AAAAAGATCTTCCATGCCAATGGAAATCAAAAGAAAGCTGGAGTG 
               
               
                   
                 GCTATTCTCATCTCAGATAAAATTGACTTCAAAATAAAGAATGTT 
               
               
                   
                 ACTCGAGATAAGGAGGGACACTACATAATGATCCAGGGGTCCATC 
               
               
                   
                 CAAGAAGAGGATATAACTATTATTAATATTTATGCACCCAACATT 
               
               
                   
                 GGCGCCCCTCAGTACATCAGGCAGCTGCTTACAGCTATCAAGGAG 
               
               
                   
                 GAAATCGACAGTAACACGATTATCGTGGGGGACTTTAACACCAGC 
               
               
                   
                 CTTACTCCGATGGATAGATCATCCAAAATGAAAATAAATAAGGAA 
               
               
                   
                 ACAGAGGCTCTTAATGACACCATTGACCAGATAGATCTGATTGAT 
               
               
                   
                 ATATATAGGACATTCCATCCAAAAACTGCCGATTACACTTTCTTC 
               
               
                   
                 AGCAGTGCGCATGGAACCTTCTCCAGGATAGATCACATCTTGGGT 
               
               
                   
                 CACAAAAGTAGCCTCAGTAAGTTTAAGAAAATTGAAATCATTAGC 
               
               
                   
                 AGCATCTTTTCTGACCATAACGCTATGCGCCTGGAGATGAATCAC 
               
               
                   
                 AGGGAGAAGAACGTAAAGAAGACAAACACCTGGAGGCTGAACAAT 
               
               
                   
                 ACGCTGCTAAATAACCAAGAGATCACTGAGGAAATCAAACAGGAA 
               
               
                   
                 ATAAAAAAATACTTGGAGACAAATGACAATGAAAACACGACCACC 
               
               
                   
                 CAGAACTTGTGGGATGCAGCTAAAGCGGTTCTGAGAGGGAAGTTT 
               
               
                   
                 ATAGCTATTCAAGCCTACCTTAAGAAACAGGAAAAATCTCAAGTG 
               
               
                   
                 AACAATTTGACCTTACACCTAAAGAAACTGGAGAAGGAGGAGCAG 
               
               
                   
                 ACCAAACCCAAAGTGAGCAGGAGGAAAGAAATCATCAAGATCAGA 
               
               
                   
                 GCCGAAATCAATGAAATAGAAACTAAGAAGACAATTGCCAAGATC 
               
               
                   
                 AATAAAACTAAATCCTGGTTCTTTGAGAAGATCAACAAAATTGAT 
               
               
                   
                 AAGCCATTAGCCAGACTCATCAAGAAAAAGAGGGAGAGGACTCAG 
               
               
                   
                 ATCAATAAGATCAGAAATGAGAAAGGGGAAGTTACAACCGACACC 
               
               
                   
                 GCGGAGATTCAGAACATCCTGAGAGACTACTACAAGCAACTTTAT 
               
               
                   
                 GCCAATAAAATGGACAACCTGGAAGAAATGGACAAATTCCTGGAA 
               
               
                   
                 AGGTATAACCTTCCCCGGCTGAACCAGGAGGAGACTGAAAATATC 
               
               
                   
                 AACCGCCCAATCACAAGTAATGAGATTGAGACTGTGATTAAGAAT 
               
               
                   
                 CTTCCAACTAACAAAAGTCCCGGCCCCGATGGCTTCACAGGTGAA 
               
               
                   
                 TTCTATCAGACCTTTCGGGAGGAGTTGACACCCATCCTTCTCAAG 
               
               
                   
                 CTCTTCCAAAAAATTGCAGAGGAGGGCACACTCCCGAACTCATTC 
               
               
                   
                 TATGAGGCCACCATCACCCTGATCCCAAAGCCCGACAAGGACACT 
               
               
                   
                 ACAAAGAAAGAAAATTACCGACCAATTTCCCTGATGAATATCGAT 
               
               
                   
                 GCCAAGATCCTCAACAAAATCTTGGCAAACAGAATCCAGCAGCAC 
               
               
                   
                 ATTAAGAGGATCATACACCACGATCAGGTGGGCTTTATCCCGGGG 
               
               
                   
                 ATGCAAGGATTCTTCAATATCCGCAAATCAATCAATGTGATCCAC 
               
               
                   
                 CATATTAACAAGTTGAAGAAGAAGAACCATATGATCATCTCCATC 
               
               
                   
                 GATGCAGAGAAAGCTTTTGACAAAATTCAACACCCATTTATGATC 
               
               
                   
                 AAAACTCTCCAGAAGGTGGGCATCGAGGGGACCTACCTCAACATA 
               
               
                   
                 ATTAAGGCCATCTATGATAAGCCCACAGCCAACATCATTCTCAAT 
               
               
                   
                 GGTGAAAAGCTGAAGGCATTTCCTCTGCGGTCCGGAACGAGACAG 
               
               
                   
                 GGATGTCCTCTCTCTCCTCTTCTGTTCAACATCGTTCTGGAAGTC 
               
               
                   
                 CTAGCCACCGCTATCCGCGAGGAAAAGGAAATTAAAGGCATACAG 
               
               
                   
                 ATTGGAAAGGAAGAGGTAAAACTGTCTCTGTTTGCGGATGATATG 
               
               
                   
                 ATACTGTACATAGAGAATCCTAAAACTGCCACCCGGAAGCTGTTG 
               
               
                   
                 GAGCTAATTAATGAGTATGGTAAGGTCGCCGGTTACAAGATTAAT 
               
               
                   
                 GCTCAGAAGTCTCTTGCTTTCCTGTACACTAATGATGAAAAGTCT 
               
               
                   
                 GAACGGGAAATTATGGAGACACTCCCCTTTACCATTGCAACCAAA 
               
               
                   
                 CGTATTAAATACCTTGGCATTAACCTGCCTAAGGAGACAAAAGAC 
               
               
                   
                 CTGTATGCTGAAAACTATAAGACACTGATGAAAGAGATTAAAGAT 
               
               
                   
                 GATACCAACCGGTGGCGGGATATCCCATGTTCTTGGATTGGCAGA 
               
               
                   
                 ATCAACATTGTGAAGATGAGCATCCTGCCCAAGGCCATCTACAGA 
               
               
                   
                 TTCAATGCCATCCCTATCAAATTACCTATGGCATTTTTTACGGAG 
               
               
                   
                 CTGGAACAGATCATCTTAAAATTTGTGTGGCGCCACAAGCGGCCC 
               
               
                   
                 CGAATCGCCAAAGCGGTCTTGAGGCAGAAGAATGGCGCTGGGGGA 
               
               
                   
                 ATCCGACTCCCTGACTTCAGATTGTACTACAAAGCTACCGTCATC 
               
               
                   
                 AAGACAATCTGGTACTGGCACAAGAACAGAAACATCGATCAGTGG 
               
               
                   
                 AACAAGATCGAAAGCCCTGAGATTAACCCCCGCACCTATGGTCAA 
               
               
                   
                 CTGATCTATGACAAAGGGGGCAAGGATATACAATGGCGCAAGGAC 
               
               
                   
                 AGCCTCTTCAATAAGTGGTGCTGGGAAAACTGGACAGCCACCTGC 
               
               
                   
                 AAGCGTATGAAGCTGGAGTACTCCCTGACACCATACACAAAAATA 
               
               
                   
                 AACTCAAAGTGGATTCGAGACCTCAATATTCGGCTGGACACTATA 
               
               
                   
                 AAACTCCTGGAGGAGAACATTGGGCGTACACTCTTTGACATTAAT 
               
               
                   
                 CATAGCAAGATCTTTTTCGATCCCCCTCCTCGTGTAATGGAAATA 
               
               
                   
                 AAAACAAAAATAAACAAGTGGGATCTGATGAAACTTCAGAGCTTT 
               
               
                   
                 TGCACCGCAAAGGAGACCATAAACAAGACGAAGCGCCAACCCTCA 
               
               
                   
                 GAATGGGAGAAAATATTTGCGAATGAGTCTACGGACAAAGGCTTA 
               
               
                   
                 ATCTCCAAAATATATAAGCAGCTCATTCAGCTCAATATCAAGGAA 
               
               
                   
                 ACAAACACCCCGATCCAAAAGTGGGCAGAGGACCTAAATCGGCAT 
               
               
                   
                 TTCTCCAAGGAAGACATCCAGACGGCCACGAAGCACATGAAGCGA 
               
               
                   
                 TGCTCAACTTCCCTGATTATTCGCGAAATGCAGATCAAGACTACT 
               
               
                   
                 ATGCGCTATCACCTCACTCCTGTTCGGATGGGCATCATCCGGAAA 
               
               
                   
                 TCTACAAACAACAAGTGCTGGAGAGGGTGTGGCGAAAAGGGAACC 
               
               
                   
                 CTCTTGCATTGTTGGTGGGAGTGTAAGTTGATCCAGCCACTATGG 
               
               
                   
                 CGGACCATATGGAGGTTCCTTAAAAAACTGAAGATTGAGCTGCCA 
               
               
                   
                 TATGACCCAGCAATCCCACTGCTGGGCATATACCCGGAGAAAACC 
               
               
                   
                 GTGATTCAGAAAGACACTTGCACCCGAATGTTCATTGCAGCATTG 
               
               
                   
                 TTTACAATAGCCAGGTCATGGAAGCAGCCTAAGTGCCCCTCGACA 
               
               
                   
                 GACGAGTGGATCAAGAAGATGTGGTACATTTATACTATGGAATAT 
               
               
                   
                 TACAGCGCCATCAAACGCAACGAAATTGGGTCTTTTCTGGAGACG 
               
               
                   
                 TGGATGGATCTAGAGACTGTCATCCAGAGTGAGGTAAGTCAGAAA 
               
               
                   
                 GAGAAGAACAAATATCGTATTTTAACGCATATTTGTGGAACCTGG 
               
               
                   
                 AAGAATGGTACAGATGAGCCGGTCTGCCGAACCGAGATTGAGACC 
               
               
                   
                 CAGATGGACTACAAAGACGATGACGACAAGTGAAGCGCTTCTAGA 
               
               
                   
                 AGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGG 
               
               
                   
                 ATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGA 
               
               
                   
                 TTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGA 
               
               
                   
                 TACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATG 
               
               
                   
                 GCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTT 
               
               
                   
                 TATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGC 
               
               
                   
                 ACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACC 
               
               
                   
                 ACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATT 
               
               
                   
                 GCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACA 
               
               
                   
                 GGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGG 
               
               
                   
                 AAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGG 
               
               
                   
                 ATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAAT 
               
               
                   
                 CCAGCGGACCTTCCTTCCCGCTGAGAGACACAAAAAATTCCAACA 
               
               
                   
                 CACTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAG 
               
               
                   
                 ATGCTCAAGGGGCTTCATGATGTCCCCATAATTTTTGGCAGAGGG 
               
               
                   
                 AAAAAGATCTCAGTGGTATTTGTGAGCCAGGGCATTGGCCTTCTG 
               
               
                   
                 ATAGGCAGCCTGCACCTGAGGAGTGCGGCCGCTTTACTTGTACAG 
               
               
                   
                 CTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGGGC 
               
               
                   
                 GGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCC 
               
               
                   
                 GTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCAC 
               
               
                   
                 GCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTCACCTTGAT 
               
               
                   
                 GCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTT 
               
               
                   
                 GTAGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTT 
               
               
                   
                 GAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCC 
               
               
                   
                 CTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTT 
               
               
                   
                 GAAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGA 
               
               
                   
                 CTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAA 
               
               
                   
                 GCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGGG 
               
               
                   
                 CACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTT 
               
               
                   
                 GCCGTAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTT 
               
               
                   
                 GTGGCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCAC 
               
               
                   
                 CCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGGGATC 
               
               
                   
                 TGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGT 
               
               
                   
                 ACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGA 
               
               
                   
                 CATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCA 
               
               
                   
                 TTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAAC 
               
               
                   
                 CGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCATG 
               
               
                   
                 GTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAG 
               
               
                   
                 TCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTA 
               
               
                   
                 CCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACT 
               
               
                   
                 TGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCAT 
               
               
                   
                 TGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATAC 
               
               
                   
                 GTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCA 
               
               
                   
                 GGCGGGCCATTTACCGTAAGTTATGTAACGGGCCTGCTGCCGGCT 
               
               
                   
                 CTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGG 
               
               
                   
                 ATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTG 
               
               
                   
                 AGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGA 
               
               
                   
                 TGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTT 
               
               
                   
                 TATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTAT 
               
               
                   
                 AAGCTGCAATAAACAAGTT 
               
               
                   
                 (SEQ ID NO: 50) 
               
               
                   
                   
               
            
           
         
       
     
     Example 19. Enriching Stably Retrotransposed Cells 
     In an effort to increase the cell yield having stably integrated nucleic acid sequence a method of sorting and culturing was attempted, as described in this example. 293T cells were electroporated with LINE1-GFP mRNA produced by IVT and cultured in vitro for at least 3 days. Expression of GFP was determined periodically using flow cytometry, as shown in  FIG.  40   . Genomic integration per genome was evaluated using quantitative PCR. Interpolations of nucleic acid encoding GFP in the genome per genome were evaluated using standard curves for GFP and a housekeeping gene (FAU). In a sorting and enrichment culture of GFP positive cells, shown in  FIG.  40   , it was evident that integration was stable for multiple cell passages (at least 18 days post EP), and considerable enrichment was possible. GFP expression was detectable in ˜1% of 293T cells 5 days post-EP. GFP+ cells were enriched to ˜28% after first sorting and was further enriched up to ˜74% of cells after 2nd sorting. ( FIG.  40   ,  FIG.  41 C ). 
     Standard curves and exemplary quantitation of genomic integrations are shown in  FIGS.  41 A and  41 B  respectively.  FIG.  41 C  shows average number of GFP integrations per genome when gated at 10{circumflex over ( )}3 units of GFP fluorescence intensity and at 10{circumflex over ( )}4 units of GFP fluorescence intensity. 
     Example 20. Titration of mRNA Concentration for Increased Transposon Mediated Integration 
     The concentration of LINE1-GFP mRNA used for electroporation was titrated for optimum genomic integration per cell in different cell types, 293T cells, K562 and THP-1 cells ( FIGS.  42 - 46   ). 100, 500, 1000, 1500 and 2000 ng/μL of mRNA were tested for GFP expression and number of integrations per cell. Concentrations higher than 1000 ng/μl cause cell death. From the results shown in  FIGS.  42 ,  43  and  44    that 1000 ng/μl causes a higher and long-term expression of GFP encoded by the retrotransposed integrated nucleic acid. Integrated DNA encoded protein expression starts to be detectable at day 3 and peaks around day 6-7 ( FIG.  45   ). However, genomic integration and expression of the LINE-1 GFP mRNA in K562 and THP-1 was quite low; integration was detected at about 0.067-0.155 per cell in K562 cells ( FIG.  46   ). (THP-1 data not shown). Higher LINE1-GFP mRNA concentrations (1500 and 2000 ng/μl) caused cell death in these cells. GFP mRNA expression in PD-0015 monocytes was detected at day 3 post electroporation, with detectable integration per cell. ( FIG.  47   ). Steps were to be taken for more extensive DNase 1 treatment, and test mRNA batches were to be evaluated for residual plasmid before electroporation. Accuracy in determination of integration levels in the genome could be improved by first enriching for integrated DNA sequence by PCR followed by paired end sequencing leading to mapping the integration sites within the genome. Next generation sequencing is considered the gold standard in this respect, which involves gDNA extraction→shearing by sonication→DNA linkers ligated onto DNA ends→nested PCR (1: one primer for linker, second to integrated DNA, 2: Illumina sequencing adapters added)→paired-end sequencing. 
     Example 21. Improvement of Integration Efficiency by Knockdown of Candidates that Prevent Transposon Mediated Integration 
     In this example, a number of endogenous candidates were knocked down using siRNA to determine if the knockdown could result in higher integration of test nucleic acid encoding GFP. Candidates included inhibitors of LINE1 retrotransposition: ADAR1, ADAR2 (ADAR1B), APOBEC3C, BRCA1, let-7 miRNA, RNase L, TASOR (HUSH complex), RAD51. siRNAs (3 per target candidate) were made, electroporated in test cells along with LINE 1-GFP mRNA and tested for alteration of the LINE-1 GFP expression by flow cytometry and its genome integration by qPCR and a cocktail of the siRNA that help increase LINE-1 GFP integration and expression was selected for further titration. Results from the different siRNAs tested are shown in  FIGS.  48 - 51   . Knockdown of ADAR1, BRCA and RNASEL tested individually induced about 2-fold increase in integration of LINE1-GFP. ADAR2, RAD51 and APOEBEC3C each led to less than 1.5-fold increase, and let7 miRNA and TASOR each led to no increase. In the study shown in  FIG.  48   , LINE-1 GFP (2000 ng/μL) was electroporated with an siBRCA at 100, 200 and 300 ng/μL in 293 cells, data shown at 4 days post electroporation. With 100 ng/μL, the integration rate was approximately ˜0.06 GFP copies per cell, and siBRCA1_s459 (100 ng/μl) increases integration by ˜2-fold. Data shown in  FIG.  49    demonstrates that at day 6 post electroporation, each of siRNASEL and siADAR1 siRNAs separately increased integration about 2-fold. On the other hand, siAPOBEC3C_s2617 increases GFP integration &lt;1.5-fold ( FIG.  50   ) at 6 days post electroporation. 
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Effect of specific knockdowns on genomic integration rate. 
               
            
           
           
               
               
               
            
               
                   
                   
                 GFP integration fold 
               
               
                   
                 Target 
                 change in 293T cells 
               
               
                   
                   
               
               
                   
                 ADAR1 
                 ~2 fold increase 
               
               
                   
                 ADAR2 
                 &lt;1.5-fold increase 
               
               
                   
                 APOEBEC3C 
                 &lt;1.5-fold increase 
               
               
                   
                 BRCA 
                 ~2 fold increase 
               
               
                   
                 Let7 miRNA 
                 No increase 
               
               
                   
                 RNASEL 
                 ~2 fold increase 
               
               
                   
                 TASOR (Hush complex) 
                 No increase 
               
               
                   
                 RAD51 
                 &lt;1.5-fold increase 
               
               
                   
                   
               
            
           
         
       
     
     siRNA against ADAR, APOEBEC3C, BRCA and RNASEL were chosen for the siRNA cocktail. Using 1000 ng/μL and 1500 ng/μL LINE1-GFP mRNA in two sets of experiments, the concentration of the siRNAs for electroporation was titrated next. It was observed that LINE1-GFP mRNA at 1500 ng/μL was slightly toxic ( FIG.  51   ). With 1000 ng/μL, 75 ng/uL of each siRNA resulted in ˜5-fold improvement of integration of GFP in 293T cells. These results were highly encouraging and support further development. Results from a similar experiment in K562 cells are shown in  FIG.  52   . 
     Example 20. Improvement of Retrotransposition Efficiency 
     Efficiency of retrotransposition can be impacted by numerous variables, such as the level of retrotransposon gene integration and subsequent transcription and translation efficiency of an integrated gene. In this example, variables such as LINE-1 mutations, mRNA sequence alterations/additions, and alterations in mRNA chemistry, are tested for their impact on increasing retrotransposition efficiency and expression of the construct in a cell. To improve the cargo gene integration, the following exemplary experiments will be conducted to test their impact on increasing retrotransposition efficiency and expression of the construct in a cell: (1) mRNA modifications, such as those that increase mRNA stability and protein expression; (2) sequence modifications, such as addition of nuclear localization sequences (NLSs) and introducing mutations in LINE-1 proteins to enhance localization and integration; (3) methods to improve cargo expression and; (4) bioinformatic analysis on various retroelements from different organisms. The learnings from these experiments are adapted across a number of human cell types, including myeloid cells, T cells, hepatocytes, cardiomyocytes, neurons and retinal pigment epithelial cells, to determine the specificity and versatility of these approaches to different cell types. In vivo delivery of the retrotransposon will also be conducted in mice. 
     Exemplary cells included in these experiments include hepatocytes, cardiomyocytes, retinal pigment epithelial cells and neurons. Primary cells and cell lines will be used and can be cultured in conditions optimal for each cell type/cell line. Test constructs include plasmid constructs and mRNA constructs comprising a sequence encoding GFP along with a promoter and a poly A sequence that is inserted in reverse orientation relative to the ORF1/2 genes in the retrotransposon complex as described elsewhere in the specification. For plasmid constructs, test constructs include constructs that contain the GFP gene in an antisense orientation interrupted by an intron in sense orientation. For mRNA constructs, test constructs include constructs without an intron. Retrotransposition conditions can include reagents to enhance integration, with the GFP-retrotransposon delivered to the cells in vitro via electroporation and/or a transfection reagent. Electroporation conditions optimized for each specific cell type are used. 
     Efficiency of integration is determined by flow cytometry, for example using gates set up based on SSC and FSC. GFP is measured in negative control and positive control to set gates for flow cytometry. An exemplary negative control used will be set at &lt;0.1% GFP+. An exemplary positive control used will be set at &gt;90% GFP+. GFP is measured in negative control and positive control to set gates for flow cytometry. An exemplary negative control used will be set at &lt;0.1% GFP+. An exemplary positive control used will be set at &gt;90% GFP+. An exemplary measurement indicative of successful integration is measurement of GFP expression, as determined by flow cytometry, in &gt;2% of cells by day 10 post retrotransposition. Another exemplary measurement indicative of successful integration is measurement of GFP expression, as determined by flow cytometry, in &gt;10% of cells by day 10 post retrotransposition. 
     Additional assays such as PCR and next generation sequencing (NGS) are performed, for example, to confirm integration. Next generation sequencing can be performed on the transfected/electroporated cells. 
     Exemplary mRNA structures that may directly impact mRNA stability and translation efficiency to which modifications will be tested to increase stability and protein expression include the 5′ CAP, the poly(A) tail, and the untranslated regions (UTRs). For example, enzymatic incorporation of the 5′CAP and use of CAP analogies, such as 7-methylguanosine, will be tested. Enzymatic incorporation of the 5′CAP may be efficient than using CAP analogs. For example, variation of the poly(A) tail length will be also be tested to determine whether the poly(A) tail length impacts translation efficiency. For example, variation of the percent GC content of mRNA will be tested to determine whether the GC content impacts translation efficiency. High GC content may increase mRNA secondary structure stability. Low GC content may reduce translatability of the mRNA. Sequence-specific mRNA variations can be tested to arrive at an optimum GC content. In another exemplary method, substituting certain codons with rare codons and inserting modified nucleotides will be undertaken in order to determine if it can affect translation efficiency. Other exemplary nucleotide modifications that will be tested to increase stability and protein expression include use of 5-methylcytidine (m5C) and pseudouridine (W) nucleotides. An exemplary measurement indicative of successful integration is measurement of GFP expression, as determined by flow cytometry, in &gt;2% of cells by day 10 post retrotransposition. Another exemplary measurement indicative of successful integration is measurement of GFP expression, as determined by flow cytometry, in &gt;10% of cells by day 10 post retrotransposition. 
     Different mRNA production processes will also be tested for increased transcription of full-length mRNAs. 
     Exemplary NLS sequence modifications will be tested, such as for enhanced localization and integration, include various NLS sequences, placement of the NLS sequences upstream or downstream of LINE-1 elements and the number of NLS sequences used. As above, GFP is measured in negative control and positive control to set gates for flow cytometry. As above, an exemplary negative control used will be set at &lt;0.1% GFP+. As above, an exemplary positive control used will be set at &gt;90% GFP+. An exemplary measurement indicative of successful cargo gene expression is measurement of increased GFP expression, as determined by flow cytometry, in &gt;10% of cells by day 10 post retrotransposition. 
     Exemplary sequence modifications that encode mutations in LINE-1 proteins that will be tested, such as for enhanced localization and integration, include K3R of ORF1 protein and Y1180A of ORF2. As above, GFP is measured in negative control and positive control to set gates for flow cytometry. As above, an exemplary negative control used will be set at &lt;0.1% GFP+. As above, an exemplary positive control used will be set at &gt;90% GFP+. An exemplary measurement indicative of successful cargo gene expression is measurement of increased GFP expression, as determined by flow cytometry, in &gt;10% of cells by day 10 post retrotransposition. 
     Example 21. Effect of Introducing a Nuclear Localization Signal in Retrotransposition by Human LINE1 Constructs 
     In this example, several constructs were generated and tested in which a nuclear localization signal was inserted in different locations as described below, and retrotransposition activity was tested in HEK293T cells. mRNA constructs containing one or more various NLSs and different numbers of total NLSs comprising the retrotransposon sequences described herein were electroporated in the 293T cells, and the number of insertions of a test gene in the genome of a cell was investigated. NLSs were individually tested at the following positions in LINE1-GFP mRNA as summarized in  FIG.  53   : (i) ORF1-N, in which an NLS was inserted at the N-terminal end of the sequence encoding ORF1 of a LINE 1-GFP construct ( FIG.  54 A ); (ii) ORF1-C, in which an NLS was inserted at the C-terminal end of the sequence encoding ORF1 of a LINE 1-GFP construct ( FIG.  55 A ), (iii) ORF2-N, in which an NLS was inserted at the N-terminal end of the sequence encoding ORF2 of a LINE1-GFP construct ( FIG.  56 A ); (iv) ORF2-N, in which an V40 NLS and a linker was inserted at the N-terminal end of the sequence encoding ORF2 of a LINE1-GFP construct ( FIG.  57 A ); and (v) ORF2-C in which an NLS was inserted at the C-terminal end of the sequence encoding ORF2 of a LINE 1-GFP construct ( FIG.  58 A ). In each case, retrotransposition using the LINE GFP NLS constructs was measured in number of GFP insertions per cell (genome). 
     Table 12A details sequences relevant to the constructs in tests described for  FIGS.  54 A- 55 C . 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 12A 
               
               
                   
                   
               
               
                   
                 NLS 
                 Amino acid sequence and nucleic acid sequence 
               
               
                   
                   
               
             
            
               
                   
                 (#3) SV40 NLS 
                 PKKKRKV (SEQ ID NO: 80) ccaaagaagaagcggaaggtc (SEQ ID NO: 81) 
               
               
                   
                   
               
               
                   
                 (#4) SV40 NLS + Linker 
                 PKKKRKV GGGS  (SEQ ID NO: 82) 
               
               
                   
                 (linker sequence underlined) 
                 ccaaagaagaagcggaaggtc ggcggcggcagc  (SEQ ID NO: 83) 
               
               
                   
                   
               
               
                   
                 (#5) Nucleoplasmin NLS 
                 KRPAATKKAGQAKKKK (SEQ ID NO: 84) 
               
               
                   
                   
                 aaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag (SEQ ID NO: 85) 
               
               
                   
                   
               
               
                   
                 (#6) Nucleoplasmin NLS + 
                 KRPAATKKAGQAKKKKGGGS (SEQ ID NO: 86) 
               
               
                   
                 Linker 
                 aaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag ggcggcggcagc  (SEQ ID 
               
               
                   
                 (linker sequence underlined) 
                 NO: 87) 
               
               
                   
                   
               
            
           
         
       
     
     As shown in  FIGS.  54 B- 54 C  and  FIGS.  55 B- 55 C , NLS insertions at N-terminal and C-terminal of ORF1 sequence abolishes retrotransposition activity of the LINE-1 constructs. On the other hand, each of the constructs shown in  FIGS.  56 A- 58 C  showed some improvement in retrotransposition, with insertion of the NLS in either ORF2 N or C terminal. 
     Table 12B details sequences relevant to the constructs in tests described for  FIGS.  56 - 56 C . 
     
       
         
           
               
               
             
               
                 TABLE 12B 
               
               
                   
               
               
                 NLS 
                 Amino acid sequence and nucleic acid sequence 
               
               
                   
               
             
            
               
                 (#3) SV40 NLS 
                 PKKKRKV (SEQ ID NO: 80) 
               
               
                   
                 ccaaagaagaagcggaaggtc (SEQ ID NO: 81) 
               
               
                   
               
               
                 (#4) Nucleoplasmin NLS 
                 KRPAATKKAGQAKKKK (SEQ ID NO: 84) 
               
               
                   
                 aaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag (SEQ ID NO: 85) 
               
               
                   
               
               
                 (#5) Linker + 
                   GGGS KRPAATKKAGQAKKKK (SEQ ID NO: 88) 
               
               
                 Nucleoplasmin NLS (linker 
                   ggcggcggcagc aaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag (SEQ 
               
               
                 sequence underlined) 
                 ID NO: 89) 
               
               
                   
               
            
           
         
       
     
     With insertion of an NLS at the N terminal end of ORF2 sequence, about 2-fold increase in number of insertions per cell. ( FIGS.  56 B, and  56 C ). However, using the SV40 NLS with the addition of a linker sequence shown in Table 12C, increases the number of GFP integrations by about 3.5 folds ( FIGS.  57 B and  57 C ). 
     Table 12C details sequences relevant to the constructs in tests described for  FIGS.  57 - 57 C . 
     
       
         
           
               
               
             
               
                 TABLE 12C 
               
               
                   
               
               
                 NLS 
                 Amino acid sequence and nucleic acid sequence 
               
               
                   
               
             
            
               
                 (#4a) SV40 NLS + Linker 
                 PKKKRKV GGGS  (SEQ ID NO: 82) 
               
               
                 (linker sequence underlined) 
                 ccaaagaagaagcggaaggtc ggcggcggcagc  (SEQ ID NO: 83) 
               
               
                   
               
            
           
         
       
     
     However, surprisingly, insertion of the NLS at the ORF2 C terminus increases the retrotransposition by about 5 fold ( FIG.  58 B- 58 C ). 
     Table 12D details sequences relevant to the constructs in tests described for  FIGS.  58 A- 58 C . 
     
       
         
           
               
               
             
               
                 TABLE 12D 
               
               
                   
               
               
                 NLS 
                 Amino acid sequence and nucleic acid sequence 
               
               
                   
               
             
            
               
                 (#3) SV40 NLS 
                 PKKKRKV (SEQ ID NO: 80) 
               
               
                   
                 ccaaagaagaagcggaaggtc (SEQ ID NO: 81) 
               
               
                   
               
               
                 (#4) Linker + SV40 
                   GGGS PKKKRKV (SEQ ID NO: 90) 
               
               
                 NLS 
                   ggcggcggcagc ccaaagaagaagcggaaggtc (SEQ ID NO: 91) 
               
               
                   
               
               
                 (#5) Nucleoplasmin 
                 KRPAATKKAGQAKKKK (SEQ ID NO: 84) 
               
               
                 NLS 
                 aaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag (SEQ ID NO: 85) 
               
               
                   
               
               
                 (#6) Linker + 
                   GGGS KRPAATKKAGQAKKKK (SEQ ID NO: 88) 
               
               
                 Nucleoplasmin NLS 
                   ggcggcggcagc aaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag (SEQ ID NO: 89) 
               
               
                   
               
            
           
         
       
     
     Insertion of both the SV40 NLS and Nucleoplasmin NLS C-terminal to ORF2 leads to &gt;5% GFP positive cells compared to less than 2% in cells that were electroporated with a LINE1-GFP mRNA construct without an NLS sequence. These results indicate that the ORF2 modifications with NLS increases retrotransposition efficiency. Most significantly, insertion at the C-terminal provides higher increase in retrotransposition of LINE1 elements. 
     Exemplary plasmids used for these experiments are shown in Table 13. 
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Exemplary plasmid sequences 
               
            
           
           
               
               
            
               
                 Name 
                 Sequence 
               
               
                   
               
               
                 &gt;RET- 
                 CTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGGCCTTG 
               
               
                 002 
                 CGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGG 
               
               
                 LINE1- 
                 GATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCGAGGAATATCGAA 
               
               
                 GFP 
                 ATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGC 
               
               
                 ORF1- 
                 TTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTGTACTCAA 
               
               
                 CSV40- 
                 GCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAACGTATA 
               
               
                 NLS 
                 ATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCATGAGTAT 
               
               
                   
                 TCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA 
               
               
                   
                 GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATCGAACTGG 
               
               
                   
                 ATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTT 
               
               
                   
                 TAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC 
               
               
                   
                 ATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGATGGCA 
               
               
                   
                 TGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCT 
               
               
                   
                 GACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC 
               
               
                   
                 CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTG 
               
               
                   
                 TAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACA 
               
               
                   
                 GTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC 
               
               
                   
                 TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGC 
               
               
                   
                 CAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACG 
               
               
                   
                 AAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTGCATTG 
               
               
                   
                 GCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAAC 
               
               
                   
                 GGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGAT 
               
               
                   
                 CGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGTGGCGC 
               
               
                   
                 TCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGATCGCGG 
               
               
                   
                 CTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCTT 
               
               
                   
                 CAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCAA 
               
               
                   
                 TCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTATTATCTA 
               
               
                   
                 ACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG 
               
               
                   
                 GAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCAT 
               
               
                   
                 TGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGT 
               
               
                   
                 GGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCT 
               
               
                   
                 ATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTACAT 
               
               
                   
                 CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGG 
               
               
                   
                 AGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGC 
               
               
                   
                 AAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGAT 
               
               
                   
                 CAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTAATACGACTCACTATA 
               
               
                   
                 GGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCG 
               
               
                   
                 CTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTT 
               
               
                   
                 CGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACA 
               
               
                   
                 AAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTG 
               
               
                   
                 AGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAG 
               
               
                   
                 TCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATG 
               
               
                   
                 AAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGG 
               
               
                   
                 ATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAA 
               
               
                   
                 ACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTG 
               
               
                   
                 CAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTA 
               
               
                   
                 TCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGT 
               
               
                   
                 GACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGG 
               
               
                   
                 GAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAA 
               
               
                   
                 AGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGT 
               
               
                   
                 GACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATAT 
               
               
                   
                 CAACCCTTGCAGAACCACGCAAAGATGccaaagaagaagcggaaggtcTGAGACAGCCGTCAGACCA 
               
               
                   
                 TCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGA 
               
               
                   
                 CCGGCTCTAACTCACATATCACCATCCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCG 
               
               
                   
                 CCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTG 
               
               
                   
                 ACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGC 
               
               
                   
                 AGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCG 
               
               
                   
                 TGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAAC 
               
               
                   
                 ATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATC 
               
               
                   
                 TGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAG 
               
               
                   
                 GCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATT 
               
               
                   
                 TATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAA 
               
               
                   
                 AGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAA 
               
               
                   
                 TTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGT 
               
               
                   
                 ACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGA 
               
               
                   
                 TTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAA 
               
               
                   
                 GGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATC 
               
               
                   
                 GATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGAC 
               
               
                   
                 GGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAAT 
               
               
                   
                 TAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATT 
               
               
                   
                 AAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACC 
               
               
                   
                 CGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCT 
               
               
                   
                 GGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCC 
               
               
                   
                 CTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTC 
               
               
                   
                 CTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCT 
               
               
                   
                 CAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATA 
               
               
                   
                 CTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATA 
               
               
                   
                 TCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACA 
               
               
                   
                 TCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTC 
               
               
                   
                 ATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCAT 
               
               
                   
                 TCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTT 
               
               
                   
                 TAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAG 
               
               
                   
                 GCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTAC 
               
               
                   
                 TCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGA 
               
               
                   
                 GGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAG 
               
               
                   
                 AACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTC 
               
               
                   
                 AGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCAT 
               
               
                   
                 AGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAA 
               
               
                   
                 AATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCAT 
               
               
                   
                 GGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCAT 
               
               
                   
                 CCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAAT 
               
               
                   
                 CAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGC 
               
               
                   
                 CTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGA 
               
               
                   
                 CATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTC 
               
               
                   
                 GATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATT 
               
               
                   
                 GGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAG 
               
               
                   
                 GTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACA 
               
               
                   
                 ATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATA 
               
               
                   
                 AGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGT 
               
               
                   
                 TAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGC 
               
               
                   
                 AGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAA 
               
               
                   
                 AAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAG 
               
               
                   
                 TTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGG 
               
               
                   
                 ATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGC 
               
               
                   
                 TCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGA 
               
               
                   
                 CCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAG 
               
               
                   
                 AGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGT 
               
               
                   
                 GGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGA 
               
               
                   
                 GTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACT 
               
               
                   
                 ATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTA 
               
               
                   
                 ACGACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGAC 
               
               
                   
                 TGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTG 
               
               
                   
                 ACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATC 
               
               
                   
                 ATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTA 
               
               
                   
                 TGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCC 
               
               
                   
                 ACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTG 
               
               
                   
                 CCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGA 
               
               
                   
                 CAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGG 
               
               
                   
                 ATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCt 
               
               
                   
                 gagagacacaaaaaattccaacacactattgcaatgaaaataaatttcctttattagccagaagtca 
               
               
                   
                 gatgctcaaggggcttcatgatgtccccataatttttggcagagggaaaaagatctcagtggtattt 
               
               
                   
                 gtgagccagggcattggccttctgataggcagcctgcacctgaggagtgcggccgctttacttgtac 
               
               
                   
                 agctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgct 
               
               
                   
                 tctcgttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagcagcacggg 
               
               
                   
                 gccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttg 
               
               
                   
                 tggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggc 
               
               
                   
                 tgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcag 
               
               
                   
                 ctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcg 
               
               
                   
                 tccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgct 
               
               
                   
                 gcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtggg 
               
               
                   
                 ccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcg 
               
               
                   
                 ccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgg 
               
               
                   
                 gcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcgggatctgacggttcactaaa 
               
               
                   
                 ccagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagt 
               
               
                   
                 tgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggg 
               
               
                   
                 gtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgc 
               
               
                   
                 atcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtca 
               
               
                   
                 tgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcat 
               
               
                   
                 atgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatgg 
               
               
                   
                 aaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgg 
               
               
                   
                 gcggtcagccaggcgggccatttaccgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCT 
               
               
                   
                 TCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAG 
               
               
                   
                 CTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGA 
               
               
                   
                 CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTAT 
               
               
                   
                 TTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGT 
               
               
                   
                 TCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCT 
               
               
                   
                 CTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTC 
               
               
                   
                 GGGCCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTAT 
               
               
                   
                 ACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCC 
               
               
                   
                 TCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAG 
               
               
                   
                 ACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCC 
               
               
                   
                 TCCCTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCA 
               
               
                   
                 GCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTC 
               
               
                   
                 CGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATT 
               
               
                   
                 CCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGA 
               
               
                   
                 ACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCA 
               
               
                   
                 CAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTT 
               
               
                   
                 TTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCT 
               
               
                   
                 GGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTG 
               
               
                   
                 CTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCA 
               
               
                   
                 TCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGC 
               
               
                   
                 GAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGAC 
               
               
                   
                 GAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCG 
               
               
                   
                 AGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTC 
               
               
                   
                 TGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGT 
               
               
                   
                 GATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTC 
               
               
                   
                 CCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCC 
               
               
                   
                 TTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACT 
               
               
                   
                 CCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTC 
               
               
                   
                 TGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGA 
               
               
                   
                 TGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTT 
               
               
                   
                 TCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGAT 
               
               
                   
                 TACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCG 
               
               
                   
                 TTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA 
               
               
                   
                 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC 
               
               
                   
                 TCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTT 
               
               
                   
                 TCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC 
               
               
                   
                 ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGT 
               
               
                   
                 AAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGC 
               
               
                   
                 GGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCT 
               
               
                   
                 GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCAC 
               
               
                   
                 CGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAA 
               
               
                   
                 GATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG 
               
               
                   
                 TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAAT 
               
               
                   
                 CTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCA 
               
               
                   
                 GCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGG 
               
               
                   
                 AAAATCTTCAAAC (SEQ ID NO: 92) 
               
               
                   
               
               
                 &gt;ret- 
                 CCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGG 
               
               
                 003- 
                 GACCCAGGAAGTCCAATCGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTA 
               
               
                 line1- 
                 AACCTAGATATTGATAGTCTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCA 
               
               
                 gfp- 
                 AGAGACAGGATCAGCAGGAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT 
               
               
                 orf1-c- 
                 TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAG 
               
               
                 linker_ 
                 ATCAGTTGGGTGCGCGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTT 
               
               
                 sv40-nls 
                 TCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCC 
               
               
                   
                 CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGT 
               
               
                   
                 ATTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCAT 
               
               
                   
                 AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACC 
               
               
                   
                 GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAG 
               
               
                   
                 CCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATT 
               
               
                   
                 AACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTT 
               
               
                   
                 GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTG 
               
               
                   
                 AGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTAT 
               
               
                   
                 CTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA 
               
               
                   
                 CTGATTAAGCATTGGTAACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGC 
               
               
                   
                 GCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCA 
               
               
                   
                 TACGTGTCCTCCTTACCAGAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATC 
               
               
                   
                 TCCGTCGTTTCGAGCTTACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCA 
               
               
                   
                 GCTATCGTCAGCTTACCTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAG 
               
               
                   
                 GTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAA 
               
               
                   
                 GACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACAC 
               
               
                   
                 ACATCCATCTTCGATGGATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAA 
               
               
                   
                 TCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGT 
               
               
                   
                 AACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCA 
               
               
                   
                 GTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCT 
               
               
                   
                 GGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCAT 
               
               
                   
                 CGCTATTACCATGCTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGG 
               
               
                   
                 GGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACT 
               
               
                   
                 TTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGT 
               
               
                   
                 CTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGA 
               
               
                   
                 CACACCCGCCAGCGGCCGCTAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGC 
               
               
                   
                 AAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTC 
               
               
                   
                 CCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGA 
               
               
                   
                 TCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGA 
               
               
                   
                 ACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAA 
               
               
                   
                 GACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAG 
               
               
                   
                 CGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCA 
               
               
                   
                 TTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGAT 
               
               
                   
                 CGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAA 
               
               
                   
                 GAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAACGCACACCCCAGC 
               
               
                   
                 GGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGA 
               
               
                   
                 GAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACG 
               
               
                   
                 GTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGG 
               
               
                   
                 AGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAA 
               
               
                   
                 GTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTC 
               
               
                   
                 AAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGAACCACGCAAAGATGggcg 
               
               
                   
                 gcggcagcccaaagaagaagcggaaggtcTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACT 
               
               
                   
                 GCATCAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGACCGGCTCTAACTCACATAT 
               
               
                   
                 CACCATCCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGG 
               
               
                   
                 ATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACC 
               
               
                   
                 GCCTCAAGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGC 
               
               
                   
                 AATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTAT 
               
               
                   
                 ATTATGGTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCG 
               
               
                   
                 GCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGAT 
               
               
                   
                 TATGGGTGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGAC 
               
               
                   
                 ACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTA 
               
               
                   
                 AGAGTACCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGG 
               
               
                   
                 CTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGC 
               
               
                   
                 GCGATCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATA 
               
               
                   
                 ACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAAC 
               
               
                   
                 AAATGAGAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAG 
               
               
                   
                 TTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGC 
               
               
                   
                 TGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGAT 
               
               
                   
                 TCGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGG 
               
               
                   
                 TTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGA 
               
               
                   
                 ACCAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCAC 
               
               
                   
                 TATTCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTT 
               
               
                   
                 CTGGATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAG 
               
               
                   
                 GAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTAC 
               
               
                   
                 AGCTGAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATA 
               
               
                   
                 GAAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGAC 
               
               
                   
                 GCGATACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAA 
               
               
                   
                 CAAGATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTT 
               
               
                   
                 ATACCTGGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAG 
               
               
                   
                 CTAAGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCC 
               
               
                   
                 ATTTATGCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATT 
               
               
                   
                 TACGATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTG 
               
               
                   
                 GAACCCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGC 
               
               
                   
                 TATTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTC 
               
               
                   
                 GCCGATGATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTT 
               
               
                   
                 CTAACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAA 
               
               
                   
                 TAATCGACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAG 
               
               
                   
                 TATCTCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGA 
               
               
                   
                 AAGAGATTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACAT 
               
               
                   
                 AGTGAAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATG 
               
               
                   
                 ACCTTCTTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAG 
               
               
                   
                 CGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTA 
               
               
                   
                 TAAAGCCACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGG 
               
               
                   
                 ACCGAACCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATA 
               
               
                   
                 AACAGTGGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAA 
               
               
                   
                 ACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAAT 
               
               
                   
                 GTCAAGCCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCG 
               
               
                   
                 GCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCT 
               
               
                   
                 TATTAAGCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACA 
               
               
                   
                 TGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGA 
               
               
                   
                 AGCAGATCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTT 
               
               
                   
                 TAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGT 
               
               
                   
                 GAGATGCAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAAT 
               
               
                   
                 CTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTG 
               
               
                   
                 CAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCC 
               
               
                   
                 TTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATA 
               
               
                   
                 CGTGTACCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCC 
               
               
                   
                 CACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAG 
               
               
                   
                 AATGACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGT 
               
               
                   
                 CTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGA 
               
               
                   
                 CGACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGG 
               
               
                   
                 ATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATG 
               
               
                   
                 TTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTAT 
               
               
                   
                 GGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTT 
               
               
                   
                 GTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCA 
               
               
                   
                 CCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGC 
               
               
                   
                 CGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCG 
               
               
                   
                 GGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCT 
               
               
                   
                 TCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattcc 
               
               
                   
                 aacacactattgcaatgaaaataaatttcctttattagccagaagtcagatgctcaaggggcttcat 
               
               
                   
                 gatgtccccataatttttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggcc 
               
               
                   
                 ttctgataggcagcctgcacctgaggagtgcggccgctttacttgtacagctcgtccatgccgagag 
               
               
                   
                 tgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgct 
               
               
                   
                 cagggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtg 
               
               
                   
                 ttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttca 
               
               
                   
                 ccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccag 
               
               
                   
                 cttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagg 
               
               
                   
                 gtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgc 
               
               
                   
                 gctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggta 
               
               
                   
                 gcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttg 
               
               
                   
                 ccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggaca 
               
               
                   
                 cgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacag 
               
               
                   
                 ctcctcgcccttgctcaccatggtggcgggatctgacggttcactaaaccagctctgcttatataga 
               
               
                   
                 cctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaa 
               
               
                   
                 gtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccc 
               
               
                   
                 cgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcga 
               
               
                   
                 tgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgcca 
               
               
                   
                 ggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtact 
               
               
                   
                 gccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgtta 
               
               
                   
                 ctatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggcc 
               
               
                   
                 atttaccgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGC 
               
               
                   
                 CCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACA 
               
               
                   
                 GCGTACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGC 
               
               
                   
                 AGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTG 
               
               
                   
                 CAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAG 
               
               
                   
                 GTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATA 
               
               
                   
                 ACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTAC 
               
               
                   
                 CGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGAT 
               
               
                   
                 TCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATC 
               
               
                   
                 CTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTT 
               
               
                   
                 TTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTA 
               
               
                   
                 CCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCC 
               
               
                   
                 TAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAAT 
               
               
                   
                 TTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGC 
               
               
                   
                 TTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCA 
               
               
                   
                 GGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCT 
               
               
                   
                 CTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTC 
               
               
                   
                 CGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCT 
               
               
                   
                 TGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGG 
               
               
                   
                 GGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCG 
               
               
                   
                 GCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGA 
               
               
                   
                 GCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCG 
               
               
                   
                 CGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCA 
               
               
                   
                 CGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGC 
               
               
                   
                 CGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTG 
               
               
                   
                 GCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGC 
               
               
                   
                 CTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCT 
               
               
                   
                 GTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAAT 
               
               
                   
                 AAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCA 
               
               
                   
                 GGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTT 
               
               
                   
                 AATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTG 
               
               
                   
                 CGTCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATC 
               
               
                   
                 ATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA 
               
               
                   
                 GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGG 
               
               
                   
                 ACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCG 
               
               
                   
                 CTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA 
               
               
                   
                 GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCC 
               
               
                   
                 CGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCA 
               
               
                   
                 CTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA 
               
               
                   
                 AGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGT 
               
               
                   
                 TACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT 
               
               
                   
                 TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA 
               
               
                   
                 CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAG 
               
               
                   
                 GATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAA 
               
               
                   
                 ACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTT 
               
               
                   
                 CATCCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCG 
               
               
                   
                 TCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTT 
               
               
                   
                 GGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATCAC 
               
               
                   
                 CCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCGAGGAATATCGAAATCGGG 
               
               
                   
                 GCGCGCCTGGTGTACCGAGAACGAT (SEQ ID NO: 93) 
               
               
                   
               
               
                 &gt;RET- 
                 TCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATC 
               
               
                 004 
                 GTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTGTA 
               
               
                 LINE1- 
                 CTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAAC 
               
               
                 GFP 
                 GTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCATG 
               
               
                 ORF1-C 
                 AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTC 
               
               
                 Nucleo- 
                 ACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATCGA 
               
               
                 plasmin- 
                 ACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGC 
               
               
                 NLS 
                 ACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTC 
               
               
                   
                 GCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGA 
               
               
                   
                 TGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA 
               
               
                   
                 CTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAA 
               
               
                   
                 CTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGAT 
               
               
                   
                 GCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG 
               
               
                   
                 CAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGG 
               
               
                   
                 CTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACT 
               
               
                   
                 GGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT 
               
               
                   
                 GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTG 
               
               
                   
                 CATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCA 
               
               
                   
                 GCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTT 
               
               
                   
                 AAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGT 
               
               
                   
                 GGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGAT 
               
               
                   
                 CGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGC 
               
               
                   
                 AGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCT 
               
               
                   
                 ATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTATT 
               
               
                   
                 ATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCAT 
               
               
                   
                 ATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCG 
               
               
                   
                 CCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAA 
               
               
                   
                 TGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGC 
               
               
                   
                 CCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGA 
               
               
                   
                 CTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAG 
               
               
                   
                 TACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCA 
               
               
                   
                 ATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATT 
               
               
                   
                 GACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGT 
               
               
                   
                 CAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTAATACGACTCA 
               
               
                   
                 CTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACA 
               
               
                   
                 ATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAAC 
               
               
                   
                 GACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCC 
               
               
                   
                 AGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAA 
               
               
                   
                 CACTGAGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGT 
               
               
                   
                 CGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACG 
               
               
                   
                 AGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGAT 
               
               
                   
                 TTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGG 
               
               
                   
                 ACTAAACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTA 
               
               
                   
                 ATGTGCAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCA 
               
               
                   
                 TATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGA 
               
               
                   
                 AGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCAC 
               
               
                   
                 GCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCC 
               
               
                   
                 TGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGAC 
               
               
                   
                 TTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATA 
               
               
                   
                 GATATCAACCCTTGCAGAACCACGCAAAGATGaaaaggccggcggccacgaaaaaggccggccaggc 
               
               
                   
                 aaaaaagaaaaagTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCATCAACTAATGAGC 
               
               
                   
                 AAAATCACCAGCTAACATCATAGTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTT 
               
               
                   
                 AACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATC 
               
               
                   
                 CAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGG 
               
               
                   
                 ATGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGAC 
               
               
                   
                 AAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCA 
               
               
                   
                 GCATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTAT 
               
               
                   
                 CAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAAT 
               
               
                   
                 ACACCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATA 
               
               
                   
                 GCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACAC 
               
               
                   
                 ATTCTTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTG 
               
               
                   
                 TCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGC 
               
               
                   
                 TGAGAATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGA 
               
               
                   
                 TTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGAT 
               
               
                   
                 ACTACCTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACG 
               
               
                   
                 CCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAA 
               
               
                   
                 ACAGGAACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAA 
               
               
                   
                 GAGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTA 
               
               
                   
                 ATAAGATAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCAT 
               
               
                   
                 CAAGAACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTAT 
               
               
                   
                 AAGCATTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTC 
               
               
                   
                 TGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGC 
               
               
                   
                 CATAATTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAA 
               
               
                   
                 CGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCT 
               
               
                   
                 TGCCCAATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAA 
               
               
                   
                 GGAAAACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAAC 
               
               
                   
                 AGAATCCAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGG 
               
               
                   
                 GCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCA 
               
               
                   
                 TATGATCATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACT 
               
               
                   
                 CTGAACAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTG 
               
               
                   
                 CTAACATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTG 
               
               
                   
                 TCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAA 
               
               
                   
                 GAGATTAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTG 
               
               
                   
                 TGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGT 
               
               
                   
                 CAGCGGCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAA 
               
               
                   
                 TCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGC 
               
               
                   
                 TGACACGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGA 
               
               
                   
                 TACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATA 
               
               
                   
                 CTTCCTAAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGC 
               
               
                   
                 TCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAG 
               
               
                   
                 CCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACT 
               
               
                   
                 AAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGA 
               
               
                   
                 TAATGCCCCACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGA 
               
               
                   
                 CAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCC 
               
               
                   
                 TTTCTTACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTA 
               
               
                   
                 TAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTC 
               
               
                   
                 AAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGC 
               
               
                   
                 TTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCG 
               
               
                   
                 CCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAA 
               
               
                   
                 GAAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATC 
               
               
                   
                 TACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGA 
               
               
                   
                 CGACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATG 
               
               
                   
                 TTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCG 
               
               
                   
                 CTTTGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTC 
               
               
                   
                 CCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTT 
               
               
                   
                 CATCGCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGG 
               
               
                   
                 ATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTT 
               
               
                   
                 CCTTCGTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGAC 
               
               
                   
                 AAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGTAAAGCGCT 
               
               
                   
                 TCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATC 
               
               
                   
                 AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCT 
               
               
                   
                 ATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCC 
               
               
                   
                 TCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCG 
               
               
                   
                 TGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCT 
               
               
                   
                 TTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGC 
               
               
                   
                 TGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCT 
               
               
                   
                 TTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTC 
               
               
                   
                 GGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaacacactattgcaat 
               
               
                   
                 gaaaataaatttcctttattagccagaagtcagatgctcaaggggcttcatgatgtccccataattt 
               
               
                   
                 ttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggccttctgataggcagcct 
               
               
                   
                 gcacctgaggagtgcggccgctttacttgtacagctcgtccatgccgagagtgatcccggcggcggt 
               
               
                   
                 cacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactgggtg 
               
               
                   
                 ctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggt 
               
               
                   
                 cggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttctt 
               
               
                   
                 ctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatg 
               
               
                   
                 ttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaact 
               
               
                   
                 tcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagcc 
               
               
                   
                 ttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgc 
               
               
                   
                 acgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatga 
               
               
                   
                 acttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggcc 
               
               
                   
                 gtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctc 
               
               
                   
                 accatggtggcgggatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacg 
               
               
                   
                 cctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttgg 
               
               
                   
                 tgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgct 
               
               
                   
                 atccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagat 
               
               
                   
                 gtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccg 
               
               
                   
                 tcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagttt 
               
               
                   
                 accgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgt 
               
               
                   
                 cattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttat 
               
               
                   
                 gtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGA 
               
               
                   
                 TCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCA 
               
               
                   
                 CAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTT 
               
               
                   
                 TATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC 
               
               
                   
                 AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGT 
               
               
                   
                 AAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCT 
               
               
                   
                 AAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAA 
               
               
                   
                 AAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGG 
               
               
                   
                 CTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCT 
               
               
                   
                 ATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAG 
               
               
                   
                 GGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAA 
               
               
                   
                 CTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCC 
               
               
                   
                 GCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCA 
               
               
                   
                 GAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAG 
               
               
                   
                 GCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTT 
               
               
                   
                 GGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTT 
               
               
                   
                 CCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAA 
               
               
                   
                 CTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCG 
               
               
                   
                 ACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTC 
               
               
                   
                 ATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTT 
               
               
                   
                 GATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGG 
               
               
                   
                 AAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTT 
               
               
                   
                 CGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTG 
               
               
                   
                 CCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGG 
               
               
                   
                 ACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGA 
               
               
                   
                 CCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTT 
               
               
                   
                 GACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCC 
               
               
                   
                 CCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGC 
               
               
                   
                 ATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAG 
               
               
                   
                 GATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGT 
               
               
                   
                 CAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGA 
               
               
                   
                 TCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCC 
               
               
                   
                 AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGA 
               
               
                   
                 CGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAG 
               
               
                   
                 GCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT 
               
               
                   
                 CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGT 
               
               
                   
                 GTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTA 
               
               
                   
                 TCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG 
               
               
                   
                 GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA 
               
               
                   
                 CGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGA 
               
               
                   
                 GTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC 
               
               
                   
                 AGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCA 
               
               
                   
                 GTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATC 
               
               
                   
                 CTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT 
               
               
                   
                 ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATT 
               
               
                   
                 TAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCA 
               
               
                   
                 GGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAG 
               
               
                   
                 CGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATCACCCGAGAGAAGTTCAAC 
               
               
                   
                 CTACATCCTCAATCCCGATCTATCCGAGATCCGAGGAATA (SEQ ID NO: 94) 
               
               
                   
               
               
                 RET- 
                 TATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATA 
               
               
                 005 
                 TCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTG 
               
               
                 LINE1- 
                 TACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCA 
               
               
                 GFP 
                 ACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCA 
               
               
                 ORF1-C 
                 TGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGC 
               
               
                 Linker_ 
                 TCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATC 
               
               
                 Nucleo- 
                 GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGA 
               
               
                 plasmin- 
                 GCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGG 
               
               
                 NLS 
                 TCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACG 
               
               
                   
                 GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACT 
               
               
                   
                 TACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGT 
               
               
                   
                 AACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG 
               
               
                   
                 ATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCC 
               
               
                   
                 GGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCC 
               
               
                   
                 GGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA 
               
               
                   
                 CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGG 
               
               
                   
                 ATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGG 
               
               
                   
                 TGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATT 
               
               
                   
                 CAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCC 
               
               
                   
                 TTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCC 
               
               
                   
                 GTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCG 
               
               
                   
                 ATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACA 
               
               
                   
                 GCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATG 
               
               
                   
                 CTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTA 
               
               
                   
                 TTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCC 
               
               
                   
                 ATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCC 
               
               
                   
                 CGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTC 
               
               
                   
                 AATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTAC 
               
               
                   
                 GCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGG 
               
               
                   
                 GACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGC 
               
               
                   
                 AGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT 
               
               
                   
                 CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCA 
               
               
                   
                 TTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACC 
               
               
                   
                 GTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTAATACGACT 
               
               
                   
                 CACTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACA 
               
               
                   
                 CAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAA 
               
               
                   
                 ACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATAT 
               
               
                   
                 CCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACA 
               
               
                   
                 AACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGT 
               
               
                   
                 GTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAA 
               
               
                   
                 CGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAG 
               
               
                   
                 ATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACG 
               
               
                   
                 GGACTAAACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGC 
               
               
                   
                 TAATGTGCAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGG 
               
               
                   
                 CATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGG 
               
               
                   
                 GAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGC 
               
               
                   
                 ACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTAC 
               
               
                   
                 CCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAG 
               
               
                   
                 ACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAA 
               
               
                   
                 TAGATATCAACCCTTGCAGAACCACGCAAAGATGggcggcggcagcaaaaggccggcggccacgaaa 
               
               
                   
                 aaggccggccaggcaaaaaagaaaaagTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGC 
               
               
                   
                 ATCAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGACCGGCTCTAACTCACATATCA 
               
               
                   
                 CCATCCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGAT 
               
               
                   
                 CAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGC 
               
               
                   
                 CTCAAGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAA 
               
               
                   
                 TTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATAT 
               
               
                   
                 TATGGTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGC 
               
               
                   
                 GCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTA 
               
               
                   
                 TGGGTGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACAC 
               
               
                   
                 GCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAG 
               
               
                   
                 AGTACCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCT 
               
               
                   
                 CAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGC 
               
               
                   
                 GATCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAAC 
               
               
                   
                 CTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAA 
               
               
                   
                 ATGAGAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTT 
               
               
                   
                 CATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTG 
               
               
                   
                 AAGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTC 
               
               
                   
                 GCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTT 
               
               
                   
                 CTTCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAAC 
               
               
                   
                 CAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTA 
               
               
                   
                 TTCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCT 
               
               
                   
                 GGATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGA 
               
               
                   
                 AGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAG 
               
               
                   
                 CTGAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGA 
               
               
                   
                 AAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGC 
               
               
                   
                 GATACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACA 
               
               
                   
                 AGATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTAT 
               
               
                   
                 ACCTGGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCT 
               
               
                   
                 AAGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCAT 
               
               
                   
                 TTATGCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTA 
               
               
                   
                 CGATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGA 
               
               
                   
                 ACCCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTA 
               
               
                   
                 TTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGC 
               
               
                   
                 CGATGATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCT 
               
               
                   
                 AACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATA 
               
               
                   
                 ATCGACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTA 
               
               
                   
                 TCTCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAA 
               
               
                   
                 GAGATTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAG 
               
               
                   
                 TGAAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGAC 
               
               
                   
                 CTTCTTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCG 
               
               
                   
                 AAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATA 
               
               
                   
                 AAGCCACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGAC 
               
               
                   
                 CGAACCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAA 
               
               
                   
                 CAGTGGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAAC 
               
               
                   
                 TCAAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGT 
               
               
                   
                 CAAGCCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGC 
               
               
                   
                 AAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTA 
               
               
                   
                 TTAAGCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATG 
               
               
                   
                 GGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAG 
               
               
                   
                 CAGATCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTA 
               
               
                   
                 GCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGA 
               
               
                   
                 GATGCAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCT 
               
               
                   
                 GGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCA 
               
               
                   
                 AGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTT 
               
               
                   
                 CGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACG 
               
               
                   
                 TGTACCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCA 
               
               
                   
                 CAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAA 
               
               
                   
                 TGACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCT 
               
               
                   
                 CAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACG 
               
               
                   
                 ACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGAT 
               
               
                   
                 CCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTT 
               
               
                   
                 GCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGG 
               
               
                   
                 CTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGT 
               
               
                   
                 CAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACC 
               
               
                   
                 ACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCG 
               
               
                   
                 CCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGG 
               
               
                   
                 GAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTC 
               
               
                   
                 TGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaa 
               
               
                   
                 cacactattgcaatgaaaataaatttcctttattagccagaagtcagatgctcaaggggcttcatga 
               
               
                   
                 tgtccccataatttttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggcctt 
               
               
                   
                 ctgataggcagcctgcacctgaggagtgcggccgctttacttgtacagctcgtccatgccgagagtg 
               
               
                   
                 atcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctca 
               
               
                   
                 gggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgtt 
               
               
                   
                 ctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcacc 
               
               
                   
                 ttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagct 
               
               
                   
                 tgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggt 
               
               
                   
                 gtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgc 
               
               
                   
                 tcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagc 
               
               
                   
                 ggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgcc 
               
               
                   
                 ggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacg 
               
               
                   
                 ctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagct 
               
               
                   
                 cctcgcccttgctcaccatggtggcgggatctgacggttcactaaaccagctctgcttatatagacc 
               
               
                   
                 tcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagt 
               
               
                   
                 cccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccg 
               
               
                   
                 tgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatg 
               
               
                   
                 actaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccagg 
               
               
                   
                 cgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgc 
               
               
                   
                 caagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttact 
               
               
                   
                 atgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccat 
               
               
                   
                 ttaccgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCC 
               
               
                   
                 TCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGC 
               
               
                   
                 GTACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG 
               
               
                   
                 TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCA 
               
               
                   
                 ATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGT 
               
               
                   
                 TTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAAC 
               
               
                   
                 CCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCG 
               
               
                   
                 GCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTC 
               
               
                   
                 AGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCT 
               
               
                   
                 TAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTT 
               
               
                   
                 CTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACC 
               
               
                   
                 TTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTA 
               
               
                   
                 ACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTT 
               
               
                   
                 TTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTT 
               
               
                   
                 TTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGG 
               
               
                   
                 TTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCT 
               
               
                   
                 GATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCG 
               
               
                   
                 GTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTG 
               
               
                   
                 CGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGG 
               
               
                   
                 CAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGC 
               
               
                   
                 GGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGC 
               
               
                   
                 ACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCG 
               
               
                   
                 CCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACG 
               
               
                   
                 GCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCG 
               
               
                   
                 GCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGC 
               
               
                   
                 GGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCT 
               
               
                   
                 TCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGT 
               
               
                   
                 TGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAA 
               
               
                   
                 AATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGG 
               
               
                   
                 ACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAA 
               
               
                   
                 TTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCG 
               
               
                   
                 TCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCAT 
               
               
                   
                 GTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGG 
               
               
                   
                 CTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC 
               
               
                   
                 TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT 
               
               
                   
                 TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG 
               
               
                   
                 TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG 
               
               
                   
                 ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACT 
               
               
                   
                 GGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAG 
               
               
                   
                 TGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA 
               
               
                   
                 CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTT 
               
               
                   
                 TGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACG 
               
               
                   
                 GGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA 
               
               
                   
                 TCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAAC 
               
               
                   
                 TTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCA 
               
               
                   
                 TCCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCGTC 
               
               
                   
                 CGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTTGG 
               
               
                   
                 CTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATCACCC 
               
               
                   
                 GAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCGAGGAA (SEQ ID NO: 
               
               
                   
                 95) 
               
               
                   
               
               
                 RET- 
                 GTCGGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAA 
               
               
                 006 
                 GTCTCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATC 
               
               
                 LINE1- 
                 CCGAATATCCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGA 
               
               
                 GFP 
                 GATCCGAGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTC 
               
               
                 ORF1- 
                 GACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATC 
               
               
                 NSV40 
                 GTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGT 
               
               
                 NLS 
                 CTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGG 
               
               
                   
                 AGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT 
               
               
                   
                 CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAG 
               
               
                   
                 TGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTT 
               
               
                   
                 TCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA 
               
               
                   
                 GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAA 
               
               
                   
                 AGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACAC 
               
               
                   
                 TGCGGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG 
               
               
                   
                 GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGC 
               
               
                   
                 GTGACACCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTAC 
               
               
                   
                 TCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC 
               
               
                   
                 TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTA 
               
               
                   
                 TCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCA 
               
               
                   
                 GGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA 
               
               
                   
                 CCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACA 
               
               
                   
                 TATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCA 
               
               
                   
                 GAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTA 
               
               
                   
                 CGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCT 
               
               
                   
                 TTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAG 
               
               
                   
                 TGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCC 
               
               
                   
                 AAGGGGTTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGA 
               
               
                   
                 TAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTA 
               
               
                   
                 GTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGC 
               
               
                   
                 CCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTT 
               
               
                   
                 CCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCAT 
               
               
                   
                 ATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACA 
               
               
                   
                 TGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGAT 
               
               
                   
                 GCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCAC 
               
               
                   
                 CCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACA 
               
               
                   
                 ACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGG 
               
               
                   
                 TTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCG 
               
               
                   
                 CTAATACGACTCACTATAGGGAGAAGTACTGCCACCATGccaaagaagaagcggaaggtcGGCAAGA 
               
               
                   
                 AGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAG 
               
               
                   
                 CTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGG 
               
               
                   
                 CGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGA 
               
               
                   
                 AGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACT 
               
               
                   
                 TAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAG 
               
               
                   
                 GAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGC 
               
               
                   
                 GCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTT 
               
               
                   
                 GATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATT 
               
               
                   
                 CAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAACGCACACCCC 
               
               
                   
                 AGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAA 
               
               
                   
                 AGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTG 
               
               
                   
                 ACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGA 
               
               
                   
                 AGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGAT 
               
               
                   
                 TAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTG 
               
               
                   
                 CTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGAACCACGCAAAGATGT 
               
               
                   
                 GAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACCAGCTA 
               
               
                   
                 ACATCATAGTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTTAACATTAACGGCCT 
               
               
                   
                 CAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGC 
               
               
                   
                 ATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTT 
               
               
                   
                 ATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGATTTCAA 
               
               
                   
                 GCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAA 
               
               
                   
                 GAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGGTCCTGT 
               
               
                   
                 CCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATTGAGCAC 
               
               
                   
                 CCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTGCATCAG 
               
               
                   
                 GCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCC 
               
               
                   
                 CACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCG 
               
               
                   
                 CACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAAC 
               
               
                   
                 CTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGGTCCACA 
               
               
                   
                 ATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTATCAAAA 
               
               
                   
                 CCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAAAGAAAA 
               
               
                   
                 CAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCC 
               
               
                   
                 ACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGAAACCCA 
               
               
                   
                 AAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAGA 
               
               
                   
                 CCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACGACAAGG 
               
               
                   
                 GCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTGTATGC 
               
               
                   
                 TAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGGCTTAAT 
               
               
                   
                 CAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCCC 
               
               
                   
                 TGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATATGGAAGA 
               
               
                   
                 GCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTC 
               
               
                   
                 TACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACTTCCGGC 
               
               
                   
                 CCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACA 
               
               
                   
                 TATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTAACATC 
               
               
                   
                 CGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTA 
               
               
                   
                 TAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAAACTCGG 
               
               
                   
                 CATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTT 
               
               
                   
                 AACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTCCCCGC 
               
               
                   
                 TTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGAT 
               
               
                   
                 ACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGGAGAAT 
               
               
                   
                 CCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGA 
               
               
                   
                 TTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATAATGGG 
               
               
                   
                 TGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTT 
               
               
                   
                 AAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGTGGA 
               
               
                   
                 AGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGAT 
               
               
                   
                 ATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAACC 
               
               
                   
                 CTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGG 
               
               
                   
                 CCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGTA 
               
               
                   
                 TTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGCCCCACATC 
               
               
                   
                 TATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACA 
               
               
                   
                 AGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTA 
               
               
                   
                 CACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAA 
               
               
                   
                 GAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGG 
               
               
                   
                 CCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAA 
               
               
                   
                 GGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTATTCATCA 
               
               
                   
                 GATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATC 
               
               
                   
                 CCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGCGAAGAA 
               
               
                   
                 GCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATAC 
               
               
                   
                 CACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGGGCTGTG 
               
               
                   
                 GCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGT 
               
               
                   
                 CTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTCGGAATC 
               
               
                   
                 TATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGT 
               
               
                   
                 TTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAAAATGTG 
               
               
                   
                 GCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACC 
               
               
                   
                 TGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATAGAATCT 
               
               
                   
                 TCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAGAAGTTGTCT 
               
               
                   
                 CCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTAC 
               
               
                   
                 AAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTG 
               
               
                   
                 CTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATC 
               
               
                   
                 CTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTG 
               
               
                   
                 TTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCG 
               
               
                   
                 CTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGC 
               
               
                   
                 TCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTC 
               
               
                   
                 GCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAG 
               
               
                   
                 CGGACCTTCCTTCCCGCtgagagacacaaaaaattccaacacactattgcaatgaaaataaatttcc 
               
               
                   
                 tttattagccagaagtcagatgctcaaggggcttcatgatgtccccataatttttggcagagggaaa 
               
               
                   
                 aagatctcagtggtatttgtgagccagggcattggccttctgataggcagcctgcacctgaggagtg 
               
               
                   
                 cggccgctttacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagca 
               
               
                   
                 ggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactgggtgctcaggtagtggtt 
               
               
                   
                 gtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacg 
               
               
                   
                 ctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggcca 
               
               
                   
                 tgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctcctt 
               
               
                   
                 gaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgg 
               
               
                   
                 gtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcgg 
               
               
                   
                 acttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcag 
               
               
                   
                 ggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagc 
               
               
                   
                 ttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgt 
               
               
                   
                 ccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcggg 
               
               
                   
                 atctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccattt 
               
               
                   
                 gcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaact 
               
               
                   
                 cccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattg 
               
               
                   
                 atgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtag 
               
               
                   
                 gaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaat 
               
               
                   
                 agggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactcc 
               
               
                   
                 acccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtca 
               
               
                   
                 atgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgGGCCTGCT 
               
               
                   
                 GCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCC 
               
               
                   
                 GCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAGA 
               
               
                   
                 TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTT 
               
               
                   
                 GTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCAT 
               
               
                   
                 TCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAA 
               
               
                   
                 TGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAG 
               
               
                   
                 GGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATG 
               
               
                   
                 CGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGC 
               
               
                   
                 CCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGAT 
               
               
                   
                 CTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGT 
               
               
                   
                 TCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGAT 
               
               
                   
                 CTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGC 
               
               
                   
                 CCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGC 
               
               
                   
                 CTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGA 
               
               
                   
                 GGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTA 
               
               
                   
                 TTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGC 
               
               
                   
                 AGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGC 
               
               
                   
                 AGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAA 
               
               
                   
                 GCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTC 
               
               
                   
                 CTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTG 
               
               
                   
                 CCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTC 
               
               
                   
                 GATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGG 
               
               
                   
                 CGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGT 
               
               
                   
                 GGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGAC 
               
               
                   
                 ATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGC 
               
               
                   
                 TTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTA 
               
               
                   
                 GTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTG 
               
               
                   
                 ACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGA 
               
               
                   
                 GTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAA 
               
               
                   
                 TAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGA 
               
               
                   
                 GATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCTA 
               
               
                   
                 TTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGG 
               
               
                   
                 AACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA 
               
               
                   
                 ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG 
               
               
                   
                 AAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT 
               
               
                   
                 TCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT 
               
               
                   
                 CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCG 
               
               
                   
                 TCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGC 
               
               
                   
                 AGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA 
               
               
                   
                 GAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTG 
               
               
                   
                 ATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGA 
               
               
                   
                 AAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT 
               
               
                   
                 CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAA 
               
               
                   
                 ATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATC 
               
               
                   
                 AGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAACT 
               
               
                   
                 CTCCAAGGCCCTC (SEQ ID NO: 96) 
               
               
                   
               
               
                 RET- 
                 GGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTC 
               
               
                 007 
                 TCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCG 
               
               
                 LINE1- 
                 AATATCCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGAT 
               
               
                 GFP 
                 CCGAGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGAC 
               
               
                 ORF1- 
                 GATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTC 
               
               
                 NSV40 
                 AGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTG 
               
               
                 NLS 
                 ATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGG 
               
               
                 Linker 
                 CTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT 
               
               
                   
                 GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGG 
               
               
                   
                 GTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCC 
               
               
                   
                 AATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG 
               
               
                   
                 CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGC 
               
               
                   
                 ATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGC 
               
               
                   
                 GGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG 
               
               
                   
                 GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTG 
               
               
                   
                 ACACCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCT 
               
               
                   
                 AGCTTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG 
               
               
                   
                 GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCA 
               
               
                   
                 TTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGC 
               
               
                   
                 AACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCG 
               
               
                   
                 ATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATAT 
               
               
                   
                 GCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAA 
               
               
                   
                 ATTTATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGC 
               
               
                   
                 GAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTT 
               
               
                   
                 TGGCAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGG 
               
               
                   
                 TCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAG 
               
               
                   
                 GGGTTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAG 
               
               
                   
                 CGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTT 
               
               
                   
                 CATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCA 
               
               
                   
                 ACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA 
               
               
                   
                 TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG 
               
               
                   
                 CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGA 
               
               
                   
                 CCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCG 
               
               
                   
                 GTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC 
               
               
                   
                 ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACT 
               
               
                   
                 CCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTT 
               
               
                   
                 AGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTA 
               
               
                   
                 ATACGACTCACTATAGGGAGAAGTACTGCCACCATGccaaagaagaagcggaaggtcggcggcggca 
               
               
                   
                 gcGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGA 
               
               
                   
                 GCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAG 
               
               
                   
                 GGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGA 
               
               
                   
                 ATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACT 
               
               
                   
                 CATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGAC 
               
               
                   
                 CAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCA 
               
               
                   
                 GGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAA 
               
               
                   
                 CCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAA 
               
               
                   
                 GACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAAC 
               
               
                   
                 GCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGT 
               
               
                   
                 GGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCT 
               
               
                   
                 ATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTA 
               
               
                   
                 ATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGA 
               
               
                   
                 GGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTC 
               
               
                   
                 AAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGAACCACG 
               
               
                   
                 CAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAAT 
               
               
                   
                 CACCAGCTAACATCATAGTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTTAACAT 
               
               
                   
                 TAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGC 
               
               
                   
                 GTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGC 
               
               
                   
                 GAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGAC 
               
               
                   
                 GGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATA 
               
               
                   
                 CAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAAC 
               
               
                   
                 AGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACC 
               
               
                   
                 ATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCA 
               
               
                   
                 CTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCT 
               
               
                   
                 TCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAA 
               
               
                   
                 GTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGA 
               
               
                   
                 ATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATT 
               
               
                   
                 GGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTAC 
               
               
                   
                 CTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTAT 
               
               
                   
                 AAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGG 
               
               
                   
                 AACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGAT 
               
               
                   
                 CGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAG 
               
               
                   
                 ATAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGA 
               
               
                   
                 ACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCA 
               
               
                   
                 TTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCA 
               
               
                   
                 CGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAA 
               
               
                   
                 TTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTA 
               
               
                   
                 TATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCC 
               
               
                   
                 AATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAA 
               
               
                   
                 ACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAAT 
               
               
                   
                 CCAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGG 
               
               
                   
                 TTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGA 
               
               
                   
                 TCATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAA 
               
               
                   
                 CAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAAC 
               
               
                   
                 ATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCC 
               
               
                   
                 TCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGAT 
               
               
                   
                 TAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTAC 
               
               
                   
                 CTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCG 
               
               
                   
                 GCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGAGAGACCGAATCCCA 
               
               
                   
                 GATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACA 
               
               
                   
                 CGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTA 
               
               
                   
                 ATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCC 
               
               
                   
                 TAAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAG 
               
               
                   
                 AAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGA 
               
               
                   
                 AGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGAC 
               
               
                   
                 AGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATG 
               
               
                   
                 CCCCACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCC 
               
               
                   
                 TCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCT 
               
               
                   
                 TACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAG 
               
               
                   
                 ACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGA 
               
               
                   
                 CGCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTG 
               
               
                   
                 TACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACT 
               
               
                   
                 TATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAA 
               
               
                   
                 CGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGC 
               
               
                   
                 CGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACC 
               
               
                   
                 ATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGC 
               
               
                   
                 GGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTG 
               
               
                   
                 GAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTG 
               
               
                   
                 CTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCG 
               
               
                   
                 CGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAA 
               
               
                   
                 GAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTC 
               
               
                   
                 GTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGC 
               
               
                   
                 ATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAG 
               
               
                   
                 AAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCT 
               
               
                   
                 CTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTG 
               
               
                   
                 GATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTT 
               
               
                   
                 GTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTG 
               
               
                   
                 TGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCG 
               
               
                   
                 GGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTG 
               
               
                   
                 GACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCA 
               
               
                   
                 TGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCC 
               
               
                   
                 TCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaacacactattgcaatgaaaa 
               
               
                   
                 taaatttcctttattagccagaagtcagatgctcaaggggcttcatgatgtccccataatttttggc 
               
               
                   
                 agagggaaaaagatctcagtggtatttgtgagccagggcattggccttctgataggcagcctgcacc 
               
               
                   
                 tgaggagtgcggccgctttacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacga 
               
               
                   
                 actccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactgggtgctcag 
               
               
                   
                 gtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcg 
               
               
                   
                 agctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgct 
               
               
                   
                 tgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgcc 
               
               
                   
                 gtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacc 
               
               
                   
                 tcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgg 
               
               
                   
                 gcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgcc 
               
               
                   
                 gtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttc 
               
               
                   
                 agggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgttta 
               
               
                   
                 cgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccat 
               
               
                   
                 ggtggcgggatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctac 
               
               
                   
                 cgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgcca 
               
               
                   
                 aaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatcca 
               
               
                   
                 cgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtact 
               
               
                   
                 gccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcatt 
               
               
                   
                 gacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgt 
               
               
                   
                 aaatactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcatta 
               
               
                   
                 ttgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaac 
               
               
                   
                 gGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCC 
               
               
                   
                 CTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGAC 
               
               
                   
                 ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTT 
               
               
                   
                 GTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAA 
               
               
                   
                 CAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAAC 
               
               
                   
                 CTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACG 
               
               
                   
                 GGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAA 
               
               
                   
                 TGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCC 
               
               
                   
                 CCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCT 
               
               
                   
                 GCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTC 
               
               
                   
                 AGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAA 
               
               
                   
                 TTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCC 
               
               
                   
                 TAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGC 
               
               
                   
                 CGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTT 
               
               
                   
                 TGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTG 
               
               
                   
                 GAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGC 
               
               
                   
                 TGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCA 
               
               
                   
                 GGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTT 
               
               
                   
                 GTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTC 
               
               
                   
                 ACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCC 
               
               
                   
                 GGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCC 
               
               
                   
                 GGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCA 
               
               
                   
                 GGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAA 
               
               
                   
                 TATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGC 
               
               
                   
                 TATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCT 
               
               
                   
                 TCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGA 
               
               
                   
                 GTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTG 
               
               
                   
                 CCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGC 
               
               
                   
                 ATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTG 
               
               
                   
                 GGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCT 
               
               
                   
                 ACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGG 
               
               
                   
                 TCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAA 
               
               
                   
                 AAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC 
               
               
                   
                 ATCACAAAAATCGAGGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTT 
               
               
                   
                 TCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCC 
               
               
                   
                 TTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG 
               
               
                   
                 TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGG 
               
               
                   
                 TAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAAC 
               
               
                   
                 AGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT 
               
               
                   
                 ACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGG 
               
               
                   
                 TAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATT 
               
               
                   
                 ACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA 
               
               
                   
                 ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTT 
               
               
                   
                 AAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAA 
               
               
                   
                 TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAAT 
               
               
                   
                 TTCCGAACTCTCCAAGGCCCTCGTC (SEQ ID NO: 97) 
               
               
                   
               
               
                 RET- 
                 GGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTC 
               
               
                 008 
                 TCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCG 
               
               
                 LINE1- 
                 AATATCCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGAT 
               
               
                 GFP 
                 CCGAGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGAC 
               
               
                 ORF1-N 
                 GATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTC 
               
               
                 Nucleo- 
                 AGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTG 
               
               
                 plasmin 
                 ATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGG 
               
               
                 NLS 
                 CTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT 
               
               
                   
                 GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGG 
               
               
                   
                 GTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCC 
               
               
                   
                 AATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG 
               
               
                   
                 CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGC 
               
               
                   
                 ATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGC 
               
               
                   
                 GGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG 
               
               
                   
                 GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTG 
               
               
                   
                 ACACCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCT 
               
               
                   
                 AGCTTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG 
               
               
                   
                 GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCA 
               
               
                   
                 TTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGC 
               
               
                   
                 AACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCG 
               
               
                   
                 ATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATAT 
               
               
                   
                 GCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAA 
               
               
                   
                 ATTTATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGC 
               
               
                   
                 GAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTT 
               
               
                   
                 TGGCAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGG 
               
               
                   
                 TCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAG 
               
               
                   
                 GGGTTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAG 
               
               
                   
                 CGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTT 
               
               
                   
                 CATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCA 
               
               
                   
                 ACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCA 
               
               
                   
                 TTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATG 
               
               
                   
                 CCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGA 
               
               
                   
                 CCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCG 
               
               
                   
                 GTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCC 
               
               
                   
                 ATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACT 
               
               
                   
                 CCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTT 
               
               
                   
                 AGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTA 
               
               
                   
                 ATACGACTCACTATAGGGAGAAGTACTGCCACCATGaaaaggccggcggccacgaaaaaggccggcc 
               
               
                   
                 aggcaaaaaagaaaaagGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAG 
               
               
                   
                 CCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGAT 
               
               
                   
                 GAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGG 
               
               
                   
                 GGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGAA 
               
               
                   
                 ATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTG 
               
               
                   
                 AGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAAA 
               
               
                   
                 GAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTA 
               
               
                   
                 CGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACTG 
               
               
                   
                 GAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAA 
               
               
                   
                 TCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCGT 
               
               
                   
                 GCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACT 
               
               
                   
                 TTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAAT 
               
               
                   
                 GGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTT 
               
               
                   
                 GAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGACA 
               
               
                   
                 ACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAAC 
               
               
                   
                 CCTTGCAGAACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCATCA 
               
               
                   
                 ACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGACCGGCTCTAACTCACATATCACCAT 
               
               
                   
                 CCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAA 
               
               
                   
                 TCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCA 
               
               
                   
                 AGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCT 
               
               
                   
                 GGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATG 
               
               
                   
                 GTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCAC 
               
               
                   
                 CTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGG 
               
               
                   
                 TGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAA 
               
               
                   
                 GAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTA 
               
               
                   
                 CCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAA 
               
               
                   
                 GGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATC 
               
               
                   
                 AAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGC 
               
               
                   
                 TGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGA 
               
               
                   
                 GAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATC 
               
               
                   
                 GCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGG 
               
               
                   
                 AGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGC 
               
               
                   
                 CGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTC 
               
               
                   
                 GAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGA 
               
               
                   
                 TTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCG 
               
               
                   
                 GGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGAT 
               
               
                   
                 ACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCG 
               
               
                   
                 AGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGA 
               
               
                   
                 GTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAG 
               
               
                   
                 GAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATA 
               
               
                   
                 CCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGAT 
               
               
                   
                 TCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCT 
               
               
                   
                 GGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGG 
               
               
                   
                 ATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTAT 
               
               
                   
                 GCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGAT 
               
               
                   
                 AAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCC 
               
               
                   
                 GCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCG 
               
               
                   
                 TCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGAT 
               
               
                   
                 GATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACT 
               
               
                   
                 TTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCG 
               
               
                   
                 ACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTC 
               
               
                   
                 GGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGA 
               
               
                   
                 TTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAA 
               
               
                   
                 GATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTC 
               
               
                   
                 TTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGT 
               
               
                   
                 CCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGC 
               
               
                   
                 CACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAA 
               
               
                   
                 CCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGT 
               
               
                   
                 GGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAA 
               
               
                   
                 GCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAG 
               
               
                   
                 CCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAG 
               
               
                   
                 ATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAA 
               
               
                   
                 GCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAA 
               
               
                   
                 AAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGA 
               
               
                   
                 TCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAA 
               
               
                   
                 AGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATG 
               
               
                   
                 CAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCA 
               
               
                   
                 ATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCT 
               
               
                   
                 GGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGAT 
               
               
                   
                 CCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTA 
               
               
                   
                 CCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAAT 
               
               
                   
                 GATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGAC 
               
               
                   
                 GAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGG 
               
               
                   
                 AGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAA 
               
               
                   
                 GTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCCGC 
               
               
                   
                 AGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTC 
               
               
                   
                 CTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTT 
               
               
                   
                 CATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGG 
               
               
                   
                 CAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCT 
               
               
                   
                 GTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTG 
               
               
                   
                 CCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAG 
               
               
                   
                 CTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCT 
               
               
                   
                 ACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaacaca 
               
               
                   
                 ctattgcaatgaaaataaatttcctttattagccagaagtcagatgctcaaggggcttcatgatgtc 
               
               
                   
                 cccataatttttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggccttctga 
               
               
                   
                 taggcagcctgcacctgaggagtgcggccgctttacttgtacagctcgtccatgccgagagtgatcc 
               
               
                   
                 cggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggc 
               
               
                   
                 ggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgc 
               
               
                   
                 tggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttga 
               
               
                   
                 tgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtg 
               
               
                   
                 ccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcg 
               
               
                   
                 ccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcct 
               
               
                   
                 ggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggct 
               
               
                   
                 gaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtg 
               
               
                   
                 gtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctga 
               
               
                   
                 acttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctc 
               
               
                   
                 gcccttgctcaccatggtggcgggatctgacggttcactaaaccagctctgcttatatagacctccc 
               
               
                   
                 accgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccg 
               
               
                   
                 ttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgag 
               
               
                   
                 tcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgacta 
               
               
                   
                 atacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcggg 
               
               
                   
                 ccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaag 
               
               
                   
                 tgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactatgg 
               
               
                   
                 gaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttac 
               
               
                   
                 cgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAG 
               
               
                   
                 ACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTAC 
               
               
                   
                 CTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAA 
               
               
                   
                 AAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAA 
               
               
                   
                 ACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTT 
               
               
                   
                 TAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCT 
               
               
                   
                 TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCAT 
               
               
                   
                 TGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCA 
               
               
                   
                 ACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAG 
               
               
                   
                 GTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGG 
               
               
                   
                 ACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGG 
               
               
                   
                 GCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTC 
               
               
                   
                 CGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTT 
               
               
                   
                 TATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTT 
               
               
                   
                 GGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCT 
               
               
                   
                 CCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATG 
               
               
                   
                 CCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGC 
               
               
                   
                 CCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCA 
               
               
                   
                 GCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGG 
               
               
                   
                 ATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCT 
               
               
                   
                 GCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGT 
               
               
                   
                 ACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAG 
               
               
                   
                 CCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGA 
               
               
                   
                 TGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTG 
               
               
                   
                 GGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCG 
               
               
                   
                 AATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTA 
               
               
                   
                 TCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTT 
               
               
                   
                 TGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATG 
               
               
                   
                 AGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAG 
               
               
                   
                 CAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATTAA 
               
               
                   
                 CCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAG 
               
               
                   
                 TCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGTGA 
               
               
                   
                 GCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC 
               
               
                   
                 GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATA 
               
               
                   
                 AAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACC 
               
               
                   
                 GGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC 
               
               
                   
                 TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 
               
               
                   
                 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCA 
               
               
                   
                 GCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT 
               
               
                   
                 GGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTT 
               
               
                   
                 CGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTT 
               
               
                   
                 TGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGT 
               
               
                   
                 CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTT 
               
               
                   
                 CACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGG 
               
               
                   
                 TCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCA 
               
               
                   
                 TAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTC (SEQ ID NO: 98) 
               
               
                   
               
               
                 RET- 
                 CAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGG 
               
               
                 009 
                 CCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAG 
               
               
                 LINE1- 
                 ATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCGAGGAATA 
               
               
                 GFP 
                 TCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATC 
               
               
                 ORF1-N 
                 GTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTGTA 
               
               
                 Nucleo- 
                 CTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAAC 
               
               
                 plasmin 
                 GTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCATG 
               
               
                 NLS 
                 AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTC 
               
               
                 Linker 
                 ACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATCGA 
               
               
                   
                 ACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGC 
               
               
                   
                 ACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTC 
               
               
                   
                 GCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGA 
               
               
                   
                 TGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA 
               
               
                   
                 CTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAA 
               
               
                   
                 CTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGAT 
               
               
                   
                 GCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG 
               
               
                   
                 CAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGG 
               
               
                   
                 CTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACT 
               
               
                   
                 GGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT 
               
               
                   
                 GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTG 
               
               
                   
                 CATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCA 
               
               
                   
                 GCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTT 
               
               
                   
                 AAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGT 
               
               
                   
                 GGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGAT 
               
               
                   
                 CGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGC 
               
               
                   
                 AGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCT 
               
               
                   
                 ATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTATT 
               
               
                   
                 ATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCAT 
               
               
                   
                 ATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCG 
               
               
                   
                 CCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAA 
               
               
                   
                 TGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGC 
               
               
                   
                 CCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGA 
               
               
                   
                 CTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAG 
               
               
                   
                 TACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCA 
               
               
                   
                 ATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATT 
               
               
                   
                 GACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGT 
               
               
                   
                 CAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTAATACGACTCA 
               
               
                   
                 CTATAGGGAGAAGTACTGCCACCATGaaaaggccggcggccacgaaaaaggccggccaggcaaaaaa 
               
               
                   
                 gaaaaagggcggcggcagcGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCT 
               
               
                   
                 AGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCG 
               
               
                   
                 ATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAA 
               
               
                   
                 GGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAG 
               
               
                   
                 AAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTC 
               
               
                   
                 TGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAA 
               
               
                   
                 AAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGAT 
               
               
                   
                 TACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAAC 
               
               
                   
                 TGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCA 
               
               
                   
                 AATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATC 
               
               
                   
                 GTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGA 
               
               
                   
                 CTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGA 
               
               
                   
                 ATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAG 
               
               
                   
                 TTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGA 
               
               
                   
                 CAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCA 
               
               
                   
                 ACCCTTGCAGAACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCAT 
               
               
                   
                 CAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGACCGGCTCTAACTCACATATCACC 
               
               
                   
                 ATCCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCA 
               
               
                   
                 AATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCT 
               
               
                   
                 CAAGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATT 
               
               
                   
                 CTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTA 
               
               
                   
                 TGGTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGC 
               
               
                   
                 ACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATG 
               
               
                   
                 GGTGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGC 
               
               
                   
                 AAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAG 
               
               
                   
                 TACCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCA 
               
               
                   
                 AAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGA 
               
               
                   
                 TCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCT 
               
               
                   
                 GCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAAT 
               
               
                   
                 GAGAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCA 
               
               
                   
                 TCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAA 
               
               
                   
                 GGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGC 
               
               
                   
                 GCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCT 
               
               
                   
                 TCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCA 
               
               
                   
                 GATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATT 
               
               
                   
                 CGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGG 
               
               
                   
                 ATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAG 
               
               
                   
                 CGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCT 
               
               
                   
                 GAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAA 
               
               
                   
                 AGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGA 
               
               
                   
                 TACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAG 
               
               
                   
                 ATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATAC 
               
               
                   
                 CTGGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAA 
               
               
                   
                 GGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTT 
               
               
                   
                 ATGCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACG 
               
               
                   
                 ATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAAC 
               
               
                   
                 CCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATT 
               
               
                   
                 CGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCG 
               
               
                   
                 ATGATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAA 
               
               
                   
                 CTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAAT 
               
               
                   
                 CGACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATC 
               
               
                   
                 TCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGA 
               
               
                   
                 GATTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTG 
               
               
                   
                 AAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCT 
               
               
                   
                 TCTTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAA 
               
               
                   
                 GTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAA 
               
               
                   
                 GCCACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCG 
               
               
                   
                 AACCATCAGAGATAATGCCCCACATCTATAATTAGCTTATATTCGATAAGCCAGAAAAGAATAAACA 
               
               
                   
                 GTGGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTC 
               
               
                   
                 AAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCA 
               
               
                   
                 AGCCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAA 
               
               
                   
                 AGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATT 
               
               
                   
                 AAGCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGG 
               
               
                   
                 AAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCA 
               
               
                   
                 GATCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGC 
               
               
                   
                 AAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGA 
               
               
                   
                 TGCAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGG 
               
               
                   
                 CAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAG 
               
               
                   
                 CTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCG 
               
               
                   
                 ATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTG 
               
               
                   
                 TACCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACA 
               
               
                   
                 ATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATG 
               
               
                   
                 ACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCA 
               
               
                   
                 GGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGAC 
               
               
                   
                 AAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCC 
               
               
                   
                 GCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGC 
               
               
                   
                 TCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCT 
               
               
                   
                 TTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCA 
               
               
                   
                 GGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCAC 
               
               
                   
                 CTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCC 
               
               
                   
                 TGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGA 
               
               
                   
                 AGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTG 
               
               
                   
                 CTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaaca 
               
               
                   
                 cactattgcaatgaaaataaatttcctttattagccagaagtcagatgctcaaggggcttcatgatg 
               
               
                   
                 tccccataatttttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggccttct 
               
               
                   
                 gataggcagcctgcacctgaggagtgcggccgctttacttgtacagctcgtccatgccgagagtgat 
               
               
                   
                 cccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagg 
               
               
                   
                 gcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttct 
               
               
                   
                 gctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcacctt 
               
               
                   
                 gatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttg 
               
               
                   
                 tgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgt 
               
               
                   
                 cgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctc 
               
               
                   
                 ctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgg 
               
               
                   
                 ctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccgg 
               
               
                   
                 tggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgct 
               
               
                   
                 gaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcc 
               
               
                   
                 tcgcccttgctcaccatggtggcgggatctgacggttcactaaaccagctctgcttatatagacctc 
               
               
                   
                 ccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcc 
               
               
                   
                 cgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtg 
               
               
                   
                 agtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgac 
               
               
                   
                 taatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcg 
               
               
                   
                 ggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgcca 
               
               
                   
                 agtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactat 
               
               
                   
                 gggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccattt 
               
               
                   
                 accgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTC 
               
               
                   
                 AGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGT 
               
               
                   
                 ACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTG 
               
               
                   
                 AAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT 
               
               
                   
                 AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTT 
               
               
                   
                 TTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCC 
               
               
                   
                 CTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGC 
               
               
                   
                 ATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAG 
               
               
                   
                 CAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTA 
               
               
                   
                 AGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCT 
               
               
                   
                 GGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTT 
               
               
                   
                 GGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAAC 
               
               
                   
                 TCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTT 
               
               
                   
                 TTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTT 
               
               
                   
                 TTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTT 
               
               
                   
                 CTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGA 
               
               
                   
                 TGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGT 
               
               
                   
                 GCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCG 
               
               
                   
                 CAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCA 
               
               
                   
                 GGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGG 
               
               
                   
                 CTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCAC 
               
               
                   
                 GTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCC 
               
               
                   
                 AGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGC 
               
               
                   
                 GATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGC 
               
               
                   
                 TGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGG 
               
               
                   
                 CGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTC 
               
               
                   
                 TATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTG 
               
               
                   
                 TTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAA 
               
               
                   
                 TGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGAC 
               
               
                   
                 AGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATT 
               
               
                   
                 AACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTC 
               
               
                   
                 AGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGT 
               
               
                   
                 GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCT 
               
               
                   
                 CCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTA 
               
               
                   
                 TAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA 
               
               
                   
                 CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA 
               
               
                   
                 TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGAC 
               
               
                   
                 CGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG 
               
               
                   
                 CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTG 
               
               
                   
                 GTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACC 
               
               
                   
                 TTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG 
               
               
                   
                 TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG 
               
               
                   
                 GTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATC 
               
               
                   
                 TTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTT 
               
               
                   
                 GGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC 
               
               
                   
                 CATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTT (SEQ ID NO: 
               
               
                   
                 99) 
               
               
                   
               
               
                 RET- 
                 GGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTG 
               
               
                 010 
                 GCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTGTACTCAAGCC 
               
               
                 LINE1- 
                 TGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAACGTATAATC 
               
               
                 GFP 
                 GAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCATGAGTATTCA 
               
               
                 ORF2- 
                 ACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA 
               
               
                 NSV40- 
                 ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATCGAACTGGATC 
               
               
                 NLS 
                 TCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTTTAA 
               
               
                   
                 AGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA 
               
               
                   
                 CACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGA 
               
               
                   
                 CAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTAGTTCTGAC 
               
               
                   
                 AACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT 
               
               
                   
                 GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAG 
               
               
                   
                 CAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAGTT 
               
               
                   
                 GATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG 
               
               
                   
                 TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAG 
               
               
                   
                 ATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAA 
               
               
                   
                 TAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTGCATTGGCG 
               
               
                   
                 CAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGA 
               
               
                   
                 TACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGATCGT 
               
               
                   
                 TTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGTGGCGCTCA 
               
               
                   
                 TTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGATCGCGGCTC 
               
               
                   
                 CCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCTTCAG 
               
               
                   
                 CTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCAATCG 
               
               
                   
                 TTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTATTATCTAACT 
               
               
                   
                 GCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAG 
               
               
                   
                 TTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGA 
               
               
                   
                 CGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGA 
               
               
                   
                 GTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATT 
               
               
                   
                 GACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTA 
               
               
                   
                 CTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTACATCAA 
               
               
                   
                 TGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGT 
               
               
                   
                 TTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAA 
               
               
                   
                 TGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCAG 
               
               
                   
                 ATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTAATACGACTCACTATAGGG 
               
               
                   
                 AGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTA 
               
               
                   
                 GCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGA 
               
               
                   
                 TGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAG 
               
               
                   
                 GGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGA 
               
               
                   
                 AATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCT 
               
               
                   
                 GAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAA 
               
               
                   
                 AGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATT 
               
               
                   
                 ACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACT 
               
               
                   
                 GGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAA 
               
               
                   
                 ATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCG 
               
               
                   
                 TGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGAC 
               
               
                   
                 TTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAA 
               
               
                   
                 TGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGT 
               
               
                   
                 TGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGAC 
               
               
                   
                 AACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAA 
               
               
                   
                 CCCTTGCAGAACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCATC 
               
               
                   
                 AACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGccaaagaagaagcggaaggtcACCG 
               
               
                   
                 GCTCTAACTCACATATCACCATCCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCGCCA 
               
               
                   
                 TCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACC 
               
               
                   
                 TGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGCAGA 
               
               
                   
                 AGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGA 
               
               
                   
                 TAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAACATC 
               
               
                   
                 TACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGG 
               
               
                   
                 ATTCTCATACGTTGATTATGGGTGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAGGCA 
               
               
                   
                 AAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTTAT 
               
               
                   
                 CGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAAAGA 
               
               
                   
                 TCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTA 
               
               
                   
                 CCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGTACC 
               
               
                   
                 ACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGATTA 
               
               
                   
                 AAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAAGGC 
               
               
                   
                 CGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGAT 
               
               
                   
                 ACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGACGGC 
               
               
                   
                 AGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAA 
               
               
                   
                 CGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATTAAG 
               
               
                   
                 AAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCGA 
               
               
                   
                 CCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCTGGA 
               
               
                   
                 AGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTC 
               
               
                   
                 AACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTCCTG 
               
               
                   
                 GTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAA 
               
               
                   
                 GCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATACTT 
               
               
                   
                 ATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCG 
               
               
                   
                 ACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACATCA 
               
               
                   
                 CGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATT 
               
               
                   
                 CAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCATTCG 
               
               
                   
                 ATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAA 
               
               
                   
                 GATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGGCC 
               
               
                   
                 TTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCG 
               
               
                   
                 AGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAGGT 
               
               
                   
                 CAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAAC 
               
               
                   
                 CTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCAGG 
               
               
                   
                 CCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGC 
               
               
                   
                 CAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAAAT 
               
               
                   
                 TACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGG 
               
               
                   
                 TTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCCC 
               
               
                   
                 AATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAA 
               
               
                   
                 AAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTG 
               
               
                   
                 ATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACAT 
               
               
                   
                 CGAGCAGTGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCGAT 
               
               
                   
                 AAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGC 
               
               
                   
                 TGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTG 
               
               
                   
                 GATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACAATA 
               
               
                   
                 CAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGA 
               
               
                   
                 TTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGTTAA 
               
               
                   
                 TAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGA 
               
               
                   
                 ATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAAAAG 
               
               
                   
                 ATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTC 
               
               
                   
                 AAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGGATG 
               
               
                   
                 GCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCC 
               
               
                   
                 ATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGACCT 
               
               
                   
                 CGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGC 
               
               
                   
                 TGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGTGGA 
               
               
                   
                 ATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGAGTA 
               
               
                   
                 TTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACTATT 
               
               
                   
                 ATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACG 
               
               
                   
                 ACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGA 
               
               
                   
                 TACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACT 
               
               
                   
                 GGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATG 
               
               
                   
                 CTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGA 
               
               
                   
                 GGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACT 
               
               
                   
                 GGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCA 
               
               
                   
                 CGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAA 
               
               
                   
                 TTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATT 
               
               
                   
                 CTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgag 
               
               
                   
                 agacacaaaaaattccaacacactattgcaatgaaaataaatttcctttattagccagaagtcagat 
               
               
                   
                 gctcaaggggcttcatgatgtccccataatttttggcagagggaaaaagatctcagtggtatttgtg 
               
               
                   
                 agccagggcattggccttctgataggcagcctgcacctgaggagtgcggccgctttacttgtacagc 
               
               
                   
                 tcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttct 
               
               
                   
                 cgttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggcc 
               
               
                   
                 gtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtgg 
               
               
                   
                 cggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgt 
               
               
                   
                 tgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctc 
               
               
                   
                 gatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtcc 
               
               
                   
                 ttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgct 
               
               
                   
                 tcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggcca 
               
               
                   
                 gggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccc 
               
               
                   
                 tcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggca 
               
               
                   
                 ccaccccggtgaacagctcctcgcccttgctcaccatggtggcgggatctgacggttcactaaacca 
               
               
                   
                 gctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgt 
               
               
                   
                 tacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtg 
               
               
                   
                 gagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatc 
               
               
                   
                 accatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgt 
               
               
                   
                 actgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatg 
               
               
                   
                 atacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaa 
               
               
                   
                 gtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcg 
               
               
                   
                 gtcagccaggcgggccatttaccgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCC 
               
               
                   
                 GCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTT 
               
               
                   
                 GACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAA 
               
               
                   
                 ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTG 
               
               
                   
                 TAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCA 
               
               
                   
                 GGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTA 
               
               
                   
                 TCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGG 
               
               
                   
                 CCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACT 
               
               
                   
                 CCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCC 
               
               
                   
                 TTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACA 
               
               
                   
                 TTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCC 
               
               
                   
                 CTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCA 
               
               
                   
                 ACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGC 
               
               
                   
                 CCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCA 
               
               
                   
                 GAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACA 
               
               
                   
                 AGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAA 
               
               
                   
                 CAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTG 
               
               
                   
                 TCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGC 
               
               
                   
                 CACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTA 
               
               
                   
                 TTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCA 
               
               
                   
                 TGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAA 
               
               
                   
                 ACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAA 
               
               
                   
                 GAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGG 
               
               
                   
                 ATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGG 
               
               
                   
                 ATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGAT 
               
               
                   
                 ATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCG 
               
               
                   
                 ATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTC 
               
               
                   
                 TAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCC 
               
               
                   
                 ACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGG 
               
               
                   
                 GGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGC 
               
               
                   
                 GGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCG 
               
               
                   
                 ACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTAC 
               
               
                   
                 GAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTG 
               
               
                   
                 CTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT 
               
               
                   
                 GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCC 
               
               
                   
                 TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT 
               
               
                   
                 CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG 
               
               
                   
                 AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAG 
               
               
                   
                 ACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT 
               
               
                   
                 GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCG 
               
               
                   
                 CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGC 
               
               
                   
                 TGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGAT 
               
               
                   
                 CCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA 
               
               
                   
                 TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTA 
               
               
                   
                 AAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCG 
               
               
                   
                 ATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAA 
               
               
                   
                 ATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTG 
               
               
                   
                 GCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATAT 
               
               
                   
                 CCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCGAG 
               
               
                   
                 GAATATCGAAATC (SEQ ID NO: 100) 
               
               
                   
               
               
                 ret-011- 
                 TGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAAT 
               
               
                 line1- 
                 ATCCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCG 
               
               
                 gfp- 
                 AGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGAT 
               
               
                 orf2-n- 
                 CCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGA 
               
               
                 sv40- 
                 TATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTGATC 
               
               
                 nls- 
                 GGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTT 
               
               
                 linker 
                 TCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT 
               
               
                   
                 TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTT 
               
               
                   
                 ACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAAT 
               
               
                   
                 GATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA 
               
               
                   
                 CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATC 
               
               
                   
                 TTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGC 
               
               
                   
                 CAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT 
               
               
                   
                 CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACA 
               
               
                   
                 CCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGC 
               
               
                   
                 TTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC 
               
               
                   
                 CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTG 
               
               
                   
                 CAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC 
               
               
                   
                 TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCGATT 
               
               
                   
                 CTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCC 
               
               
                   
                 AGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATT 
               
               
                   
                 TATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAA 
               
               
                   
                 CAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGG 
               
               
                   
                 CAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCA 
               
               
                   
                 TAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGG 
               
               
                   
                 TTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAGCGA 
               
               
                   
                 TTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTTCAT 
               
               
                   
                 AGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACG 
               
               
                   
                 ACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTG 
               
               
                   
                 ACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCA 
               
               
                   
                 AGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCT 
               
               
                   
                 TATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCGGTT 
               
               
                   
                 TTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATT 
               
               
                   
                 GACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCG 
               
               
                   
                 CCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGT 
               
               
                   
                 GAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTAATA 
               
               
                   
                 CGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCA 
               
               
                   
                 AGACACAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGAT 
               
               
                   
                 GGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAA 
               
               
                   
                 GATATCCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTA 
               
               
                   
                 TCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGA 
               
               
                   
                 GGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAG 
               
               
                   
                 ATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGC 
               
               
                   
                 AGGAGATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGA 
               
               
                   
                 AAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGG 
               
               
                   
                 CAAGCTAATGTGCAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGA 
               
               
                   
                 AAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTC 
               
               
                   
                 CAGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCT 
               
               
                   
                 CTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCT 
               
               
                   
                 GCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGC 
               
               
                   
                 AATAATAGATATCAACCCTTGCAGAACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTA 
               
               
                   
                 GGAAGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGccaaagaaga 
               
               
                   
                 agcggaaggtcggcggcggcagcACCGGCTCTAACTCACATATCACCATCCTTACACTTAACATTAA 
               
               
                   
                 CGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTT 
               
               
                   
                 TGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAA 
               
               
                   
                 AGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGA 
               
               
                   
                 TTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAG 
               
               
                   
                 CAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGG 
               
               
                   
                 TCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATT 
               
               
                   
                 GAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTG 
               
               
                   
                 CATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCA 
               
               
                   
                 GCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTG 
               
               
                   
                 CAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATC 
               
               
                   
                 AAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGG 
               
               
                   
                 TCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTA 
               
               
                   
                 TCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAA 
               
               
                   
                 AGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAAC 
               
               
                   
                 AGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGA 
               
               
                   
                 AACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATA 
               
               
                   
                 GACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACG 
               
               
                   
                 ACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTT 
               
               
                   
                 GTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGG 
               
               
                   
                 CTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTA 
               
               
                   
                 ACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATAT 
               
               
                   
                 GGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAAT 
               
               
                   
                 TCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACT 
               
               
                   
                 TCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCA 
               
               
                   
                 ACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTT 
               
               
                   
                 AACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGATCA 
               
               
                   
                 TCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAA 
               
               
                   
                 ACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATT 
               
               
                   
                 ATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCT 
               
               
                   
                 CCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAA 
               
               
                   
                 AGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTG 
               
               
                   
                 GAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCT 
               
               
                   
                 ATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGAT 
               
               
                   
                 AATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGA 
               
               
                   
                 GACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATA 
               
               
                   
                 AGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAA 
               
               
                   
                 AGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAA 
               
               
                   
                 ACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGA 
               
               
                   
                 ATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGC 
               
               
                   
                 CTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGCCC 
               
               
                   
                 CACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCT 
               
               
                   
                 TCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTAC 
               
               
                   
                 ACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACA 
               
               
                   
                 CTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGC 
               
               
                   
                 CCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTAC 
               
               
                   
                 TGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTAT 
               
               
                   
                 TCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGA 
               
               
                   
                 ATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGC 
               
               
                   
                 GAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATG 
               
               
                   
                 CGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGG 
               
               
                   
                 GCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAA 
               
               
                   
                 ATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTC 
               
               
                   
                 GGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGG 
               
               
                   
                 CCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAA 
               
               
                   
                 AATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTT 
               
               
                   
                 GGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATA 
               
               
                   
                 GAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAGAAG 
               
               
                   
                 TTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTG 
               
               
                   
                 GATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGAT 
               
               
                   
                 ACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTA 
               
               
                   
                 TAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGC 
               
               
                   
                 ACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGA 
               
               
                   
                 CTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGAC 
               
               
                   
                 AGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGG 
               
               
                   
                 CTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCA 
               
               
                   
                 ATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaacacactattgcaatgaaaataa 
               
               
                   
                 atttcctttattagccagaagtcagatgctcaaggggcttcatgatgtccccataatttttggcaga 
               
               
                   
                 gggaaaaagatctcagtggtatttgtgagccagggcattggccttctgataggcagcctgcacctga 
               
               
                   
                 ggagtgcggccgctttacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaact 
               
               
                   
                 ccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactgggtgctcaggta 
               
               
                   
                 gtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagc 
               
               
                   
                 tgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgt 
               
               
                   
                 cggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtc 
               
               
                   
                 ctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcg 
               
               
                   
                 gcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggca 
               
               
                   
                 tggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgta 
               
               
                   
                 ggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagg 
               
               
                   
                 gtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgt 
               
               
                   
                 cgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggt 
               
               
                   
                 ggcgggatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgc 
               
               
                   
                 ccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaa 
               
               
                   
                 caaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgc 
               
               
                   
                 ccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgcc 
               
               
                   
                 aagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgac 
               
               
                   
                 gtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaa 
               
               
                   
                 tactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattg 
               
               
                   
                 acgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgGG 
               
               
                   
                 CCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTT 
               
               
                   
                 TGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATG 
               
               
                   
                 ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTG 
               
               
                   
                 AAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAA 
               
               
                   
                 TTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTC 
               
               
                   
                 TACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGT 
               
               
                   
                 CTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGC 
               
               
                   
                 CTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCA 
               
               
                   
                 ACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCA 
               
               
                   
                 GGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGA 
               
               
                   
                 AGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTA 
               
               
                   
                 AGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAA 
               
               
                   
                 CTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGA 
               
               
                   
                 GGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGC 
               
               
                   
                 AAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAG 
               
               
                   
                 AGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGT 
               
               
                   
                 CAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGA 
               
               
                   
                 CGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTC 
               
               
                   
                 ACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACC 
               
               
                   
                 TTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGC 
               
               
                   
                 TACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGT 
               
               
                   
                 CTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGC 
               
               
                   
                 TCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATAT 
               
               
                   
                 CATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTAT 
               
               
                   
                 CAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCC 
               
               
                   
                 TCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTT 
               
               
                   
                 CTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCT 
               
               
                   
                 TCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATT 
               
               
                   
                 GTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGA 
               
               
                   
                 AGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACT 
               
               
                   
                 TGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCC 
               
               
                   
                 TTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAG 
               
               
                   
                 GCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC 
               
               
                   
                 ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCC 
               
               
                   
                 CCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 
               
               
                   
                 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG 
               
               
                   
                 TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAA 
               
               
                   
                 CTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGG 
               
               
                   
                 ATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA 
               
               
                   
                 CTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAG 
               
               
                   
                 CTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACG 
               
               
                   
                 CGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG 
               
               
                   
                 AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAA 
               
               
                   
                 TTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGC 
               
               
                   
                 TTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTC 
               
               
                   
                 CGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCT 
               
               
                   
                 CTCGAACGAACTATCGCAAGTCTCT (SEQ ID NO: 101) 
               
               
                   
               
               
                 RET- 
                 GACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATC 
               
               
                 012 
                 GTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGT 
               
               
                 LINE1- 
                 CTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGG 
               
               
                 GFP 
                 AGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT 
               
               
                 ORF2-N 
                 CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAG 
               
               
                 Nucleo- 
                 TGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTT 
               
               
                 plasmin- 
                 TCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA 
               
               
                 NLS 
                 GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAA 
               
               
                   
                 AGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACAC 
               
               
                   
                 TGCGGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG 
               
               
                   
                 GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGC 
               
               
                   
                 GTGACACCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTAC 
               
               
                   
                 TCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC 
               
               
                   
                 TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTA 
               
               
                   
                 TCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCA 
               
               
                   
                 GGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA 
               
               
                   
                 CCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACA 
               
               
                   
                 TATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCA 
               
               
                   
                 GAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTA 
               
               
                   
                 CGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCT 
               
               
                   
                 TTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAG 
               
               
                   
                 TGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCC 
               
               
                   
                 AAGGGGTTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGA 
               
               
                   
                 TAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTA 
               
               
                   
                 GTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGC 
               
               
                   
                 CCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTT 
               
               
                   
                 CCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCAT 
               
               
                   
                 ATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACA 
               
               
                   
                 TGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGAT 
               
               
                   
                 GCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCAC 
               
               
                   
                 CCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACA 
               
               
                   
                 ACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGG 
               
               
                   
                 TTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCG 
               
               
                   
                 CTAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGGA 
               
               
                   
                 ATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTC 
               
               
                   
                 CTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTC 
               
               
                   
                 CGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCA 
               
               
                   
                 CCCGTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCT 
               
               
                   
                 TCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAA 
               
               
                   
                 GACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGA 
               
               
                   
                 GTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGA 
               
               
                   
                 CGTAGAAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTG 
               
               
                   
                 GCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTG 
               
               
                   
                 CCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGC 
               
               
                   
                 TCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAG 
               
               
                   
                 ACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCAC 
               
               
                   
                 GAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACA 
               
               
                   
                 GATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATG 
               
               
                   
                 GAGCGCAATAATAGATATCAACCCTTGCAGAACCACGCAAAGATGTGAGACAGCCGTCAGACCATCA 
               
               
                   
                 AGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGaaaa 
               
               
                   
                 ggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaagACCGGCTCTAACTCACATATCAC 
               
               
                   
                 CATCCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATC 
               
               
                   
                 AAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCC 
               
               
                   
                 TCAAGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAAT 
               
               
                   
                 TCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATT 
               
               
                   
                 ATGGTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCG 
               
               
                   
                 CACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTAT 
               
               
                   
                 GGGTGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACG 
               
               
                   
                 CAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGA 
               
               
                   
                 GTACCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTC 
               
               
                   
                 AAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCG 
               
               
                   
                 ATCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACC 
               
               
                   
                 TGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAA 
               
               
                   
                 TGAGAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTC 
               
               
                   
                 ATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGA 
               
               
                   
                 AGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCG 
               
               
                   
                 CGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTC 
               
               
                   
                 TTCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACC 
               
               
                   
                 AGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTAT 
               
               
                   
                 TCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTG 
               
               
                   
                 GATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAA 
               
               
                   
                 GCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGC 
               
               
                   
                 TGAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAA 
               
               
                   
                 AAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCG 
               
               
                   
                 ATACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAA 
               
               
                   
                 GATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATA 
               
               
                   
                 CCTGGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTA 
               
               
                   
                 AGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATT 
               
               
                   
                 TATGCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTAC 
               
               
                   
                 GATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAA 
               
               
                   
                 CCCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTAT 
               
               
                   
                 TCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCC 
               
               
                   
                 GATGATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTA 
               
               
                   
                 ACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAA 
               
               
                   
                 TCGACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTAT 
               
               
                   
                 CTCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAG 
               
               
                   
                 AGATTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGT 
               
               
                   
                 GAAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACC 
               
               
                   
                 TTCTTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGA 
               
               
                   
                 AGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAA 
               
               
                   
                 AGCCACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACC 
               
               
                   
                 GAACCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAAC 
               
               
                   
                 AGTGGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACT 
               
               
                   
                 CAAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTC 
               
               
                   
                 AAGCCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCA 
               
               
                   
                 AAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTAT 
               
               
                   
                 TAAGCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGG 
               
               
                   
                 GAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGC 
               
               
                   
                 AGATCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAG 
               
               
                   
                 CAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAG 
               
               
                   
                 ATGCAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTG 
               
               
                   
                 GCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAA 
               
               
                   
                 GCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTC 
               
               
                   
                 GATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGT 
               
               
                   
                 GTACCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCAC 
               
               
                   
                 AATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAAT 
               
               
                   
                 GACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTC 
               
               
                   
                 AGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGA 
               
               
                   
                 CAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATC 
               
               
                   
                 CGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTG 
               
               
                   
                 CTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGC 
               
               
                   
                 TTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTC 
               
               
                   
                 AGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCA 
               
               
                   
                 CCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGC 
               
               
                   
                 CTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGG 
               
               
                   
                 AAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCT 
               
               
                   
                 GCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaac 
               
               
                   
                 acactattgcaatgaaaataaatttcctttattagccagaagtcagatgctcaaggggcttcatgat 
               
               
                   
                 gtccccataatttttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggccttc 
               
               
                   
                 tgataggcagcctgcacctgaggagtgcggccgctttacttgtacagctcgtccatgccgagagtga 
               
               
                   
                 tcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcag 
               
               
                   
                 ggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttc 
               
               
                   
                 tgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcacct 
               
               
                   
                 tgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagctt 
               
               
                   
                 gtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtg 
               
               
                   
                 tcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgct 
               
               
                   
                 cctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcg 
               
               
                   
                 gctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccg 
               
               
                   
                 gtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgc 
               
               
                   
                 tgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctc 
               
               
                   
                 ctcgcccttgctcaccatggtggcgggatctgacggttcactaaaccagctctgcttatatagacct 
               
               
                   
                 cccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtc 
               
               
                   
                 ccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgt 
               
               
                   
                 gagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatga 
               
               
                   
                 ctaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggc 
               
               
                   
                 gggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgcc 
               
               
                   
                 aagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttacta 
               
               
                   
                 tgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatt 
               
               
                   
                 taccgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCT 
               
               
                   
                 CAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCG 
               
               
                   
                 TACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGT 
               
               
                   
                 GAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAA 
               
               
                   
                 TAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTT 
               
               
                   
                 TTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACC 
               
               
                   
                 CCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGG 
               
               
                   
                 CATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCA 
               
               
                   
                 GCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTT 
               
               
                   
                 AAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTC 
               
               
                   
                 TGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCT 
               
               
                   
                 TGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAA 
               
               
                   
                 CTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTT 
               
               
                   
                 TTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTT 
               
               
                   
                 TTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGT 
               
               
                   
                 TCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTG 
               
               
                   
                 ATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGG 
               
               
                   
                 TGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGC 
               
               
                   
                 GCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGC 
               
               
                   
                 AGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCG 
               
               
                   
                 GCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCA 
               
               
                   
                 CGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGC 
               
               
                   
                 CAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGG 
               
               
                   
                 CGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGG 
               
               
                   
                 CTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCG 
               
               
                   
                 GCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTT 
               
               
                   
                 CTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTT 
               
               
                   
                 GTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAA 
               
               
                   
                 ATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGA 
               
               
                   
                 CAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAAT 
               
               
                   
                 TAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGT 
               
               
                   
                 CAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATG 
               
               
                   
                 TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC 
               
               
                   
                 TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACT 
               
               
                   
                 ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTT 
               
               
                   
                 ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT 
               
               
                   
                 ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGA 
               
               
                   
                 CCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTG 
               
               
                   
                 GCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT 
               
               
                   
                 GGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC 
               
               
                   
                 CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTT 
               
               
                   
                 GTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGG 
               
               
                   
                 GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGAT 
               
               
                   
                 CTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACT 
               
               
                   
                 TGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCAT 
               
               
                   
                 CCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCGTCC 
               
               
                   
                 GATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTTGGC 
               
               
                   
                 TATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATCACCCG 
               
               
                   
                 AGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCGAGGAATATCGAAATCGGGGCG 
               
               
                   
                 CGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTC (SEQ ID NO: 102) 
               
               
                   
               
               
                 RET- 
                 AGATCCGAGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCT 
               
               
                 013 
                 CGACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAAT 
               
               
                 LINE1- 
                 CGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAG 
               
               
                 GFP 
                 TCTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAG 
               
               
                 ORF2-N 
                 GAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCT 
               
               
                 Nucleo- 
                 TCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGA 
               
               
                 plasmin 
                 GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCT 
               
               
                 NLS 
                 TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCA 
               
               
                 Linker 
                 AGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAA 
               
               
                   
                 AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACA 
               
               
                   
                 CTGCGGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACAT 
               
               
                   
                 GGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG 
               
               
                   
                 CGTGACACCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTA 
               
               
                   
                 CTCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG 
               
               
                   
                 CTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT 
               
               
                   
                 ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTC 
               
               
                   
                 AGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTA 
               
               
                   
                 ACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCAC 
               
               
                   
                 ATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACC 
               
               
                   
                 AGAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTT 
               
               
                   
                 ACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACC 
               
               
                   
                 TTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTA 
               
               
                   
                 GTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCC 
               
               
                   
                 CAAGGGGTTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGG 
               
               
                   
                 ATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATT 
               
               
                   
                 AGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG 
               
               
                   
                 CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTT 
               
               
                   
                 TCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCA 
               
               
                   
                 TATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTAC 
               
               
                   
                 ATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGA 
               
               
                   
                 TGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCA 
               
               
                   
                 CCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAAC 
               
               
                   
                 AACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTG 
               
               
                   
                 GTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCC 
               
               
                   
                 GCTAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGG 
               
               
                   
                 AATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGT 
               
               
                   
                 CCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACT 
               
               
                   
                 CCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATC 
               
               
                   
                 ACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGC 
               
               
                   
                 TTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGA 
               
               
                   
                 AGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAG 
               
               
                   
                 AGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCG 
               
               
                   
                 ACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCT 
               
               
                   
                 GGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGT 
               
               
                   
                 GCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCG 
               
               
                   
                 CTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGA 
               
               
                   
                 GACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCA 
               
               
                   
                 CGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAAC 
               
               
                   
                 AGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATAT 
               
               
                   
                 GGAGCGCAATAATAGATATCAACCCTTGCAGAACCACGCAAAGATGTGAGACAGCCGTCAGACCATC 
               
               
                   
                 AAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGaaa 
               
               
                   
                 aggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaagggcggcggcagcACCGGCTCTA 
               
               
                   
                 ACTCACATATCACCATCCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCT 
               
               
                   
                 GGCCAGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGA 
               
               
                   
                 GATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAG 
               
               
                   
                 CCGGAGTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGA 
               
               
                   
                 AGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAACATCTACGCG 
               
               
                   
                 CCAAACACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTC 
               
               
                   
                 ATACGTTGATTATGGGTGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGT 
               
               
                   
                 AAATAAAGACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTTATCGCACT 
               
               
                   
                 CTTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAAAGATCGATC 
               
               
                   
                 ATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAATTACCTGTC 
               
               
                   
                 AGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGG 
               
               
                   
                 AAGCTTAATAACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGATTAAAATGT 
               
               
                   
                 TCTTCGAAACAAATGAGAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTG 
               
               
                   
                 CAGAGGCAAGTTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCGATACTCTC 
               
               
                   
                 ACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGA 
               
               
                   
                 TCACAAAGATTCGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTC 
               
               
                   
                 TCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATTAAGAAGAAG 
               
               
                   
                 CGCGAAAAGAACCAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCCGACCGAGA 
               
               
                   
                 TCCAGACCACTATTCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGAT 
               
               
                   
                 GGACACTTTTCTGGATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGC 
               
               
                   
                 CCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGG 
               
               
                   
                 ACGGGTTTACAGCTGAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTT 
               
               
                   
                 TCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATACTTATTCCC 
               
               
                   
                 AAACCAGGACGCGATACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATATCGACGCTA 
               
               
                   
                 AAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACATCACGACCA 
               
               
                   
                 GGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCATTCAACAC 
               
               
                   
                 ATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCATTCGATAAGA 
               
               
                   
                 TTCAGCAGCCATTTATGCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTTAAGATTAT 
               
               
                   
                 TCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCG 
               
               
                   
                 CTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGC 
               
               
                   
                 TGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCT 
               
               
                   
                 TTCCTTGTTCGCCGATGATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTT 
               
               
                   
                 AAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTC 
               
               
                   
                 TGTACACAAATAATCGACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAA 
               
               
                   
                 AAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAG 
               
               
                   
                 CCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCA 
               
               
                   
                 GAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATCCCAATTAA 
               
               
                   
                 ACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAATCAAAAGAGA 
               
               
                   
                 GCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTA 
               
               
                   
                 AGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGACATCGACCA 
               
               
                   
                 GTGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCGATAAGCCA 
               
               
                   
                 GAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCA 
               
               
                   
                 TATGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGGTGGATCAA 
               
               
                   
                 GGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACAATACAAGAT 
               
               
                   
                 ATAGGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAAGATTGATA 
               
               
                   
                 AGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGTTAATAGGCA 
               
               
                   
                 GCCCACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCAGAATATAT 
               
               
                   
                 AACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAAAAGATATGA 
               
               
                   
                 ACAGGCATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTT 
               
               
                   
                 GGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGGATGGCAATT 
               
               
                   
                 ATCAAGAAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCT 
               
               
                   
                 GGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCT 
               
               
                   
                 TGAGATTCCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGAGCTGTTGT 
               
               
                   
                 TACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGC 
               
               
                   
                 CTAAGTGCCCCACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGAGTATTACGC 
               
               
                   
                 AGCAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACTATTATTCTG 
               
               
                   
                 AGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAACGACTACA 
               
               
                   
                 AAGACGATGACGACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAAT 
               
               
                   
                 CGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATT 
               
               
                   
                 CTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTG 
               
               
                   
                 CTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTT 
               
               
                   
                 GTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGG 
               
               
                   
                 GGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGG 
               
               
                   
                 AACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGT 
               
               
                   
                 GGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGC 
               
               
                   
                 GGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacac 
               
               
                   
                 aaaaaattccaacacactattgcaatgaaaataaatttcctttattagccagaagtcagatgctcaa 
               
               
                   
                 ggggcttcatgatgtccccataatttttggcagagggaaaaagatctcagtggtatttgtgagccag 
               
               
                   
                 ggcattggccttctgataggcagcctgcacctgaggagtgcggccgctttacttgtacagctcgtcc 
               
               
                   
                 atgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttgg 
               
               
                   
                 ggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgcc 
               
               
                   
                 gatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatc 
               
               
                   
                 ttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagt 
               
               
                   
                 tgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcg 
               
               
                   
                 gttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaag 
               
               
                   
                 aagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgt 
               
               
                   
                 ggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcac 
               
               
                   
                 gggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccc 
               
               
                   
                 tcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccc 
               
               
                   
                 cggtgaacagctcctcgcccttgctcaccatggtggcgggatctgacggttcactaaaccagctctg 
               
               
                   
                 cttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgac 
               
               
                   
                 attttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagact 
               
               
                   
                 tggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatg 
               
               
                   
                 gtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactggg 
               
               
                   
                 cataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacac 
               
               
                   
                 ttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccct 
               
               
                   
                 attggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagc 
               
               
                   
                 caggcgggccatttaccgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCT 
               
               
                   
                 TCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGA 
               
               
                   
                 CTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA 
               
               
                   
                 ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCA 
               
               
                   
                 TTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGA 
               
               
                   
                 GGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTA 
               
               
                   
                 TCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGAT 
               
               
                   
                 TGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACA 
               
               
                   
                 TATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCA 
               
               
                   
                 GAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTT 
               
               
                   
                 TAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATC 
               
               
                   
                 TCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATA 
               
               
                   
                 GTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATC 
               
               
                   
                 GCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTA 
               
               
                   
                 GTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGG 
               
               
                   
                 ATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACA 
               
               
                   
                 ATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGA 
               
               
                   
                 CCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGAC 
               
               
                   
                 GGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGC 
               
               
                   
                 GAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTG 
               
               
                   
                 ATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCG 
               
               
                   
                 CATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCAT 
               
               
                   
                 CAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCG 
               
               
                   
                 TCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCAT 
               
               
                   
                 CGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCT 
               
               
                   
                 GAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGC 
               
               
                   
                 AGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTG 
               
               
                   
                 CCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTC 
               
               
                   
                 CTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTG 
               
               
                   
                 GGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGG 
               
               
                   
                 CTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACG 
               
               
                   
                 CTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTT 
               
               
                   
                 CATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCG 
               
               
                   
                 TTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA 
               
               
                   
                 ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCC 
               
               
                   
                 GACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGC 
               
               
                   
                 TCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC 
               
               
                   
                 CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA 
               
               
                   
                 CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACA 
               
               
                   
                 GAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGC 
               
               
                   
                 TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAG 
               
               
                   
                 CGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTG 
               
               
                   
                 ATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT 
               
               
                   
                 TATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTAT 
               
               
                   
                 ATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGT 
               
               
                   
                 CTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTC 
               
               
                   
                 AAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGGC 
               
               
                   
                 CTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGA 
               
               
                   
                 TCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCG (SEQ ID NO: 
               
               
                   
                 103) 
               
               
                   
               
               
                 RET- 
                 AGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATC 
               
               
                 014 
                 CAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGA 
               
               
                 LINE1- 
                 TCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCAAAC 
               
               
                 GFP 
                 ATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTA 
               
               
                 ORF2- 
                 TTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGA 
               
               
                 CSV40- 
                 TGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT 
               
               
                 NLS 
                 GAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGG 
               
               
                   
                 TATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTT 
               
               
                   
                 GGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT 
               
               
                   
                 GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGG 
               
               
                   
                 AGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT 
               
               
                   
                 GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACCTTGCGT 
               
               
                   
                 AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAGGCGG 
               
               
                   
                 ATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG 
               
               
                   
                 AGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC 
               
               
                   
                 GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAG 
               
               
                   
                 GTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGC 
               
               
                   
                 GACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCC 
               
               
                   
                 CACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGGCTCT 
               
               
                   
                 ATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAA 
               
               
                   
                 TCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCATTAG 
               
               
                   
                 CTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAA 
               
               
                   
                 CCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCAATCGTTGCGTTACACACACAAAAA 
               
               
                   
                 ACCAACACACATCCATCTTCGATGGATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGA 
               
               
                   
                 TCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTAC 
               
               
                   
                 GGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTT 
               
               
                   
                 CCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCC 
               
               
                   
                 ACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATG 
               
               
                   
                 GCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTA 
               
               
                   
                 TTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTG 
               
               
                   
                 ACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCA 
               
               
                   
                 ACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGG 
               
               
                   
                 TGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCAT 
               
               
                   
                 CCACTCGACACACCCGCCAGCGGCCGCTAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGG 
               
               
                   
                 CAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGAGCGT 
               
               
                   
                 TCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGAT 
               
               
                   
                 TTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGAATTT 
               
               
                   
                 CGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACTCATG 
               
               
                   
                 GAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGC 
               
               
                   
                 TCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGA 
               
               
                   
                 GAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAACCTG 
               
               
                   
                 CGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAAGACA 
               
               
                   
                 TCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAACGCAC 
               
               
                   
                 ACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGTGGAG 
               
               
                   
                 ATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTC 
               
               
                   
                 GGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTAATAT 
               
               
                   
                 CCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGT 
               
               
                   
                 GAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGG 
               
               
                   
                 AACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGAACCACGCAAA 
               
               
                   
                 GATGTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACC 
               
               
                   
                 AGCTAACATCATAGTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTTAACATTAAC 
               
               
                   
                 GGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTTT 
               
               
                   
                 GTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAA 
               
               
                   
                 GATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGAT 
               
               
                   
                 TTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAGC 
               
               
                   
                 AGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGGT 
               
               
                   
                 CCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATTG 
               
               
                   
                 AGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTGC 
               
               
                   
                 ATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAG 
               
               
                   
                 CGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTGC 
               
               
                   
                 AAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCA 
               
               
                   
                 AGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGGT 
               
               
                   
                 CCACAATGAGATGAAGGGAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTAT 
               
               
                   
                 CAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAAA 
               
               
                   
                 GAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACA 
               
               
                   
                 GACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGAA 
               
               
                   
                 ACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAG 
               
               
                   
                 ACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACGA 
               
               
                   
                 CAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTG 
               
               
                   
                 TATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGGC 
               
               
                   
                 TTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAA 
               
               
                   
                 CTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATATG 
               
               
                   
                 GAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATT 
               
               
                   
                 CCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACTT 
               
               
                   
                 CCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAA 
               
               
                   
                 CAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTA 
               
               
                   
                 ACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGATCAT 
               
               
                   
                 CTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAAA 
               
               
                   
                 CTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTA 
               
               
                   
                 TCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTC 
               
               
                   
                 CCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAA 
               
               
                   
                 GGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGG 
               
               
                   
                 AGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTA 
               
               
                   
                 TAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATA 
               
               
                   
                 ATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAG 
               
               
                   
                 ACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAA 
               
               
                   
                 GTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAAA 
               
               
                   
                 GTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAA 
               
               
                   
                 CAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAA 
               
               
                   
                 TAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCC 
               
               
                   
                 TGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGCCCC 
               
               
                   
                 ACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTT 
               
               
                   
                 CAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTACA 
               
               
                   
                 CCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACAC 
               
               
                   
                 TGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGCC 
               
               
                   
                 CAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACT 
               
               
                   
                 GCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTATT 
               
               
                   
                 CATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAA 
               
               
                   
                 TAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGCG 
               
               
                   
                 AAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATGC 
               
               
                   
                 GATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGGG 
               
               
                   
                 CTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAA 
               
               
                   
                 TCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTCG 
               
               
                   
                 GAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGGC 
               
               
                   
                 CTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAAA 
               
               
                   
                 ATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTTG 
               
               
                   
                 GGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATAG 
               
               
                   
                 AATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGccaaagaagaagcggaag 
               
               
                   
                 gtcTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCC 
               
               
                   
                 GCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGC 
               
               
                   
                 TCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCT 
               
               
                   
                 TTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCA 
               
               
                   
                 GGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCAC 
               
               
                   
                 CTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCC 
               
               
                   
                 TGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGA 
               
               
                   
                 AGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTG 
               
               
                   
                 CTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaaca 
               
               
                   
                 cactattgcaatgaaaataaatttcctttattagccagaagtcagatgctcaaggggcttcatgatg 
               
               
                   
                 tccccataatttttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggccttct 
               
               
                   
                 gataggcagcctgcacctgaggagtgcggccgctttacttgtacagctcgtccatgccgagagtgat 
               
               
                   
                 cccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagg 
               
               
                   
                 gcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttct 
               
               
                   
                 gctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcacctt 
               
               
                   
                 gatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttg 
               
               
                   
                 tgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgt 
               
               
                   
                 cgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctc 
               
               
                   
                 ctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcgg 
               
               
                   
                 ctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccgg 
               
               
                   
                 tggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgct 
               
               
                   
                 gaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcc 
               
               
                   
                 tcgcccttgctcaccatggtggcgggatctgacggttcactaaaccagctctgcttatatagacctc 
               
               
                   
                 ccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcc 
               
               
                   
                 cgttgattttggtgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtg 
               
               
                   
                 agtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgac 
               
               
                   
                 taatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcg 
               
               
                   
                 ggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgcca 
               
               
                   
                 agtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactat 
               
               
                   
                 gggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccattt 
               
               
                   
                 accgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTC 
               
               
                   
                 AGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGT 
               
               
                   
                 ACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTG 
               
               
                   
                 AAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT 
               
               
                   
                 AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTT 
               
               
                   
                 TTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCC 
               
               
                   
                 CTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGC 
               
               
                   
                 ATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAG 
               
               
                   
                 CAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTA 
               
               
                   
                 AGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCT 
               
               
                   
                 GGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTT 
               
               
                   
                 GGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAAC 
               
               
                   
                 TCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTT 
               
               
                   
                 TTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTT 
               
               
                   
                 TTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTT 
               
               
                   
                 CTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGA 
               
               
                   
                 TGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGT 
               
               
                   
                 GCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCG 
               
               
                   
                 CAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCA 
               
               
                   
                 GGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGG 
               
               
                   
                 CTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCAC 
               
               
                   
                 GTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCC 
               
               
                   
                 AGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGC 
               
               
                   
                 GATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGC 
               
               
                   
                 TGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGG 
               
               
                   
                 CGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTC 
               
               
                   
                 TATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTG 
               
               
                   
                 TTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAA 
               
               
                   
                 TGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGAC 
               
               
                   
                 AGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATT 
               
               
                   
                 AACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTC 
               
               
                   
                 AGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGT 
               
               
                   
                 GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCT 
               
               
                   
                 CCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTA 
               
               
                   
                 TAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA 
               
               
                   
                 CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA 
               
               
                   
                 TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGAC 
               
               
                   
                 CGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG 
               
               
                   
                 CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTG 
               
               
                   
                 GTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACC 
               
               
                   
                 TTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG 
               
               
                   
                 TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG 
               
               
                   
                 GTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATC 
               
               
                   
                 TTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTT 
               
               
                   
                 GGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC 
               
               
                   
                 CATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCGTCCG 
               
               
                   
                 ATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTTGGCT 
               
               
                   
                 ATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATCACCCGA 
               
               
                   
                 GAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCGAGGAATATCGAAATCGGGGCGC 
               
               
                   
                 GCCTGGTGTACCG (SEQ ID NO: 104) 
               
               
                   
               
               
                 RET- 
                 TCGACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAA 
               
               
                 015 
                 TCGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATA 
               
               
                 LINE1- 
                 GTCTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCA 
               
               
                 GFP 
                 GGAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCC 
               
               
                 ORF2- 
                 TTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCG 
               
               
                 C_Linker_ 
                 AGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGC 
               
               
                 SV40- 
                 TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGC 
               
               
                 NLS 
                 AAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGA 
               
               
                   
                 AAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC 
               
               
                   
                 ACTGCGGCCAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACA 
               
               
                   
                 TGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGA 
               
               
                   
                 GCGTGACACCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTT 
               
               
                   
                 ACTCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGC 
               
               
                   
                 GCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGG 
               
               
                   
                 TATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT 
               
               
                   
                 CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGT 
               
               
                   
                 AACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCA 
               
               
                   
                 CATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTAC 
               
               
                   
                 CAGAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCT 
               
               
                   
                 TACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTAC 
               
               
                   
                 CTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCT 
               
               
                   
                 AGTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCC 
               
               
                   
                 CCAAGGGGTTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATG 
               
               
                   
                 GATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCAT 
               
               
                   
                 TAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACC 
               
               
                   
                 GCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACT 
               
               
                   
                 TTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATC 
               
               
                   
                 ATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTA 
               
               
                   
                 CATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTG 
               
               
                   
                 ATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCC 
               
               
                   
                 ACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAA 
               
               
                   
                 CAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                 GGTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGC 
               
               
                   
                 CGCTAATACGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGG 
               
               
                   
                 GAATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAG 
               
               
                   
                 TCCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAAC 
               
               
                   
                 TCCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCAT 
               
               
                   
                 CACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAG 
               
               
                   
                 CTTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGG 
               
               
                   
                 AAGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACA 
               
               
                   
                 GAGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGC 
               
               
                   
                 GACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACC 
               
               
                   
                 TGGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCG 
               
               
                   
                 TGCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCC 
               
               
                   
                 GCTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCG 
               
               
                   
                 AGACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCC 
               
               
                   
                 ACGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAA 
               
               
                   
                 CAGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATA 
               
               
                   
                 TGGAGCGCAATAATAGATATCAACCCTTGCAGAACCACGCAAAGATGTGAGACAGCCGTCAGACCAT 
               
               
                   
                 CAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGAC 
               
               
                   
                 CGGCTCTAACTCACATATCACCATCCTTACACTTAACATTAACGGCCTCAACTCAGCTATCAAGCGC 
               
               
                   
                 CATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAGAGACCCACCTGA 
               
               
                   
                 CCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTTATCAGGCGAACGGTAAGCA 
               
               
                   
                 GAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACCAAAATTAAGCGT 
               
               
                   
                 GATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAGAACTTACCATATTGAACA 
               
               
                   
                 TCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCTGCAGCGAGATCT 
               
               
                   
                 GGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATTGAGCACCCTGGATCGCAGCACCAGG 
               
               
                   
                 CAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATCTCATTGATATTT 
               
               
                   
                 ATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCACATCATACATACTCAAA 
               
               
                   
                 GATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAGATAATTACAAAT 
               
               
                   
                 TACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGACCCAGAGCCGGAGTA 
               
               
                   
                 CCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGGTCCACAATGAGATGAAGGCAGAGAT 
               
               
                   
                 TAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTATCAAAACCTTTGGGATGCCTTTAAG 
               
               
                   
                 GCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGAGATCTAAGATCG 
               
               
                   
                 ATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCAAGGCGTCAAGACG 
               
               
                   
                 GCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACTCTTCAGAAAATT 
               
               
                   
                 AACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGGCACGACTGATTA 
               
               
                   
                 AGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACGACAAGGGCGACATCACTACTGACCC 
               
               
                   
                 GACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTGTATGCTAACAAGCTTGAGAACCTG 
               
               
                   
                 GAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGGCTTAATCAAGAGGAAGTCGAGTCCC 
               
               
                   
                 TCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGACAAAGAAATCTCC 
               
               
                   
                 TGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATATGGAAGAGCTTGTACCGTTTCTGCTC 
               
               
                   
                 AAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAGCTTCTATAATAC 
               
               
                   
                 TTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACTTCCGGCCCATTAGTCTCATGAATAT 
               
               
                   
                 CGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACATATTAAGAAATTGATACAT 
               
               
                   
                 CACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTAACATCCGGAAGAGTATTAACGTCA 
               
               
                   
                 TTCAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTATAGACGCGGAAAAGGCATT 
               
               
                   
                 CGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAAACTCGGCATCGACGGAACATATTTT 
               
               
                   
                 AAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTTAACGGCCAAAAGCTCGAGG 
               
               
                   
                 CCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTTTAATATTGTACT 
               
               
                   
                 CGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTCGGGAAGGAAGAG 
               
               
                   
                 GTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGGAGAATCCTATTGTGTCTGCTCAGA 
               
               
                   
                 ACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATTAACGTCCAGAAATCTCA 
               
               
                   
                 GGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATAATGGGTGAGCTTCCGTTTGTCATA 
               
               
                   
                 GCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAAGATTTGTTTAAGGAAA 
               
               
                   
                 ATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGTGGAAGAATATCCCCTGTTCATG 
               
               
                   
                 GGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATATCGCTTTAACGCCATC 
               
               
                   
                 CCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAACCCTTAAATTTATATGGAATC 
               
               
                   
                 AAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGGGATTACTTTGCC 
               
               
                   
                 TGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGTATTGGTATCAGAATAGAGAC 
               
               
                   
                 ATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAATTACCTTATATTCG 
               
               
                   
                 ATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACAAGTGGTGTTGGGAGAATTG 
               
               
                   
                 GCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAAATCAACAGTAGG 
               
               
                   
                 TGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAAGAGAATCTTGGGATCACAA 
               
               
                   
                 TACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGCCACTAAGGATAA 
               
               
                   
                 GATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAAGGAGACCACGATCAGAGTT 
               
               
                   
                 AATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGGGGTTGATAAGCA 
               
               
                   
                 GAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATCCCATCAAGAAGTGGGCAAA 
               
               
                   
                 AGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATGAAGAAGTGTAGT 
               
               
                   
                 TCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATACCACCTTACCCCAGTGAGGA 
               
               
                   
                 TGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGATTGGCACCCTGCT 
               
               
                   
                 CCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGCTTTCTGAGGGAC 
               
               
                   
                 CTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTAACGAATACAAGA 
               
               
                   
                 GCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTACGATAGCTAAGACGTG 
               
               
                   
                 GAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAAAATGTGGCATATTTATACCATGGAG 
               
               
                   
                 TATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGATGAAGCTGGAGACTA 
               
               
                   
                 TTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCTCATTGGTGGTAA 
               
               
                   
                 CGACTACAAAGACGATGACGACAAGggcggcggcagcccaaagaagaagcggaaggtcTAAAGCGCT 
               
               
                   
                 TCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATC 
               
               
                   
                 AACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCT 
               
               
                   
                 ATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCC 
               
               
                   
                 TCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCG 
               
               
                   
                 TGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCT 
               
               
                   
                 TTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGC 
               
               
                   
                 TGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCT 
               
               
                   
                 TTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTC 
               
               
                   
                 GGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaacacactattgcaat 
               
               
                   
                 gaaaataaatttcctttattagccagaagtcagatgctcaaggggcttcatgatgtccccataattt 
               
               
                   
                 ttggcagagggaaaaagatctcagtggtatttgtgagccagggcattggccttctgataggcagcct 
               
               
                   
                 gcacctgaggagtgcggccgctttacttgtacagctcgtccatgccgagagtgatcccggcggcggt 
               
               
                   
                 cacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactgggtg 
               
               
                   
                 ctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggt 
               
               
                   
                 cggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttctt 
               
               
                   
                 ctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatg 
               
               
                   
                 ttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaact 
               
               
                   
                 tcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagcc 
               
               
                   
                 ttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgc 
               
               
                   
                 acgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatga 
               
               
                   
                 acttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggcc 
               
               
                   
                 gtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctc 
               
               
                   
                 accatggtggcgggatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacg 
               
               
                   
                 cctaccgcccatttgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttgg 
               
               
                   
                 tgccaaaacaaactcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgct 
               
               
                   
                 atccacgcccattgatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagat 
               
               
                   
                 gtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccg 
               
               
                   
                 tcattgacgtcaatagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagttt 
               
               
                   
                 accgtaaatactccacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgt 
               
               
                   
                 cattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttat 
               
               
                   
                 gtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGA 
               
               
                   
                 TCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCA 
               
               
                   
                 CAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTT 
               
               
                   
                 TATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC 
               
               
                   
                 AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGT 
               
               
                   
                 AAAACCTCTACAAATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCT 
               
               
                   
                 AAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAA 
               
               
                   
                 AAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGG 
               
               
                   
                 CTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCT 
               
               
                   
                 ATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAG 
               
               
                   
                 GGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAA 
               
               
                   
                 CTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCC 
               
               
                   
                 GCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCA 
               
               
                   
                 GAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAG 
               
               
                   
                 GCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTT 
               
               
                   
                 GGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTT 
               
               
                   
                 CCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAA 
               
               
                   
                 CTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCG 
               
               
                   
                 ACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTC 
               
               
                   
                 ATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTT 
               
               
                   
                 GATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGG 
               
               
                   
                 AAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTT 
               
               
                   
                 CGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTG 
               
               
                   
                 CCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGG 
               
               
                   
                 ACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGA 
               
               
                   
                 CCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTT 
               
               
                   
                 GACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCC 
               
               
                   
                 CCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGC 
               
               
                   
                 ATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAG 
               
               
                   
                 GATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGT 
               
               
                   
                 CAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGA 
               
               
                   
                 TCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCC 
               
               
                   
                 AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGA 
               
               
                   
                 CGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAG 
               
               
                   
                 GCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT 
               
               
                   
                 CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGT 
               
               
                   
                 GTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTA 
               
               
                   
                 TCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG 
               
               
                   
                 GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA 
               
               
                   
                 CGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGA 
               
               
                   
                 GTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC 
               
               
                   
                 AGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCA 
               
               
                   
                 GTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATC 
               
               
                   
                 CTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT 
               
               
                   
                 ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATT 
               
               
                   
                 TAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCA 
               
               
                   
                 GGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAG 
               
               
                   
                 CGCCTATCGCCAGGTATTACTCCAATCCCGAATATCCGAGATCGGGATCACCCGAGAGAAGTTCAAC 
               
               
                   
                 CTACATCCTCAATCCCGATCTATCCGAGATCCGAGGAATATCGAAATCGGGGCGCGCCTGGTGTACC 
               
               
                   
                 GAGAACGATCCTCTCAGTGCGAGTC (SEQ ID NO: 105) 
               
               
                   
               
               
                 ret-016- 
                 ACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGG 
               
               
                 line1- 
                 AATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTA 
               
               
                 gfp- 
                 CCGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGC 
               
               
                 orf- 
                 AAACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCC 
               
               
                 c_nucleo- 
                 CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAA 
               
               
                 plasmin- 
                 AAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGAT 
               
               
                 nls 
                 CCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGC 
               
               
                   
                 GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATG 
               
               
                   
                 ACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATG 
               
               
                   
                 CAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATTGGAGGACCG 
               
               
                   
                 AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG 
               
               
                   
                 AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACCTT 
               
               
                   
                 GCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAG 
               
               
                   
                 GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAAT 
               
               
                   
                 CTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCG 
               
               
                   
                 TATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG 
               
               
                   
                 ATAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTG 
               
               
                   
                 ATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACT 
               
               
                   
                 TGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGG 
               
               
                   
                 CTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCAT 
               
               
                   
                 CGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCA 
               
               
                   
                 TTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAA 
               
               
                   
                 AAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCAATCGTTGCGTTACACACACA 
               
               
                   
                 AAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGC 
               
               
                   
                 CAGATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC 
               
               
                   
                 TTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTA 
               
               
                   
                 TGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACT 
               
               
                   
                 GCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTA 
               
               
                   
                 AATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTA 
               
               
                   
                 CGTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGG 
               
               
                   
                 TTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAA 
               
               
                   
                 ATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGT 
               
               
                   
                 ACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTA 
               
               
                   
                 CCATCCACTCGACACACCCGCCAGCGGCCGCTAATACGACTCACTATAGGGAGAAGTACTGCCACCA 
               
               
                   
                 TGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGA 
               
               
                   
                 GCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAG 
               
               
                   
                 GGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGA 
               
               
                   
                 ATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACT 
               
               
                   
                 CATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGAC 
               
               
                   
                 CAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCA 
               
               
                   
                 GGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAA 
               
               
                   
                 CCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAA 
               
               
                   
                 GACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAAC 
               
               
                   
                 GCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGT 
               
               
                   
                 GGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCT 
               
               
                   
                 ATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTA 
               
               
                   
                 ATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGA 
               
               
                   
                 GGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTC 
               
               
                   
                 AAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGAACCACG 
               
               
                   
                 CAAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAAT 
               
               
                   
                 CACCAGCTAACATCATAGTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTTAACAT 
               
               
                   
                 TAACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGC 
               
               
                   
                 GTTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGC 
               
               
                   
                 GAAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGAC 
               
               
                   
                 GGATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATA 
               
               
                   
                 CAGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAAC 
               
               
                   
                 AGGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACC 
               
               
                   
                 ATTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCA 
               
               
                   
                 CTGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCT 
               
               
                   
                 TCAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAA 
               
               
                   
                 GTGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGA 
               
               
                   
                 ATCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATT 
               
               
                   
                 GGGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTAC 
               
               
                   
                 CTATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTAT 
               
               
                   
                 AAAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGG 
               
               
                   
                 AACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGAT 
               
               
                   
                 CGAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAG 
               
               
                   
                 ATAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGA 
               
               
                   
                 ACGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCA 
               
               
                   
                 TTTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCA 
               
               
                   
                 CGGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAA 
               
               
                   
                 TTAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTA 
               
               
                   
                 TATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCC 
               
               
                   
                 AATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAA 
               
               
                   
                 ACTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAAT 
               
               
                   
                 CCAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGG 
               
               
                   
                 TTTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGA 
               
               
                   
                 TCATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAA 
               
               
                   
                 CAAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAAC 
               
               
                   
                 ATTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCC 
               
               
                   
                 TCTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGAT 
               
               
                   
                 TAAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTAC 
               
               
                   
                 CTGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCG 
               
               
                   
                 GCTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCA 
               
               
                   
                 GATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACA 
               
               
                   
                 CGAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTA 
               
               
                   
                 ATAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCC 
               
               
                   
                 TAAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAG 
               
               
                   
                 AAAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGA 
               
               
                   
                 AGAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGAC 
               
               
                   
                 AGCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATG 
               
               
                   
                 CCCCACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCC 
               
               
                   
                 TCTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCT 
               
               
                   
                 TACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAG 
               
               
                   
                 ACACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGA 
               
               
                   
                 CGCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTG 
               
               
                   
                 TACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACT 
               
               
                   
                 TATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAA 
               
               
                   
                 CGAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGC 
               
               
                   
                 CGCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACC 
               
               
                   
                 ATGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGC 
               
               
                   
                 GGGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTG 
               
               
                   
                 GAAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTG 
               
               
                   
                 CTCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCG 
               
               
                   
                 CGGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAA 
               
               
                   
                 GAAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTC 
               
               
                   
                 GTTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGC 
               
               
                   
                 ATAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGaaaaggccggcggc 
               
               
                   
                 cacgaaaaaggccggccaggcaaaaaagaaaaagTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCAC 
               
               
                   
                 TGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTG 
               
               
                   
                 AAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCC 
               
               
                   
                 TTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTG 
               
               
                   
                 TCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACG 
               
               
                   
                 CAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCT 
               
               
                   
                 CCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTG 
               
               
                   
                 GGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTG 
               
               
                   
                 CCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCC 
               
               
                   
                 TTCCCGCtgagagacacaaaaaattccaacacactattgcaatgaaaataaatttcctttattagcc 
               
               
                   
                 agaagtcagatgctcaaggggcttcatgatgtccccataatttttggcagagggaaaaagatctcag 
               
               
                   
                 tggtatttgtgagccagggcattggccttctgataggcagcctgcacctgaggagtgcggccgcttt 
               
               
                   
                 acttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtg 
               
               
                   
                 atcgcgcttctcgttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagc 
               
               
                   
                 agcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcct 
               
               
                   
                 cgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagac 
               
               
                   
                 gttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatg 
               
               
                   
                 cccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagt 
               
               
                   
                 tgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaa 
               
               
                   
                 gtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacg 
               
               
                   
                 agggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtagg 
               
               
                   
                 tggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgac 
               
               
                   
                 caggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcgggatctgacggt 
               
               
                   
                 tcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatgg 
               
               
                   
                 ggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacg 
               
               
                   
                 tcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgcc 
               
               
                   
                 aaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtccca 
               
               
                   
                 taaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgta 
               
               
                   
                 cttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgac 
               
               
                   
                 gtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggg 
               
               
                   
                 gtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgGGCCTGCTGCCGGCTCTG 
               
               
                   
                 CGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGC 
               
               
                   
                 CTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGATG 
               
               
                   
                 AGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTAT 
               
               
                   
                 TGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG 
               
               
                   
                 TTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTG 
               
               
                   
                 GCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG 
               
               
                   
                 TGCCCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCG 
               
               
                   
                 CGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCAT 
               
               
                   
                 ACGTGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTT 
               
               
                   
                 CGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACT 
               
               
                   
                 TTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCA 
               
               
                   
                 ATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGC 
               
               
                   
                 CCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCT 
               
               
                   
                 GAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAAC 
               
               
                   
                 ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATG 
               
               
                   
                 ACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCC 
               
               
                   
                 GGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTA 
               
               
                   
                 TCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGG 
               
               
                   
                 ACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAA 
               
               
                   
                 AGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGAC 
               
               
                   
                 CACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATG 
               
               
                   
                 ATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCC 
               
               
                   
                 CGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGC 
               
               
                   
                 CGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGG 
               
               
                   
                 CTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTAT 
               
               
                   
                 CGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGC 
               
               
                   
                 CCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAG 
               
               
                   
                 GTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCA 
               
               
                   
                 TTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCAT 
               
               
                   
                 GCTGGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTG 
               
               
                   
                 TCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGT 
               
               
                   
                 TCTCCGATTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA 
               
               
                   
                 AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTC 
               
               
                   
                 AAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTC 
               
               
                   
                 GTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG 
               
               
                   
                 TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGG 
               
               
                   
                 CTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCC 
               
               
                   
                 AACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT 
               
               
                   
                 ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATT 
               
               
                   
                 TGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAA 
               
               
                   
                 CAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT 
               
               
                   
                 CTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGG 
               
               
                   
                 GATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTT 
               
               
                   
                 AAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCAC 
               
               
                   
                 CTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCC 
               
               
                   
                 CTCGTCGGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCG 
               
               
                   
                 CAAGTCTCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCA 
               
               
                   
                 ATCCCGAATATCCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATC 
               
               
                   
                 CGAGATCCGAGGAATATCGAAATCGGGGCGCGCCTGGTGT (SEQ ID NO: 106) 
               
               
                   
               
               
                 RET- 
                 CCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGGA 
               
               
                 017 
                 ATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTAC 
               
               
                 LINE1- 
                 CGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGCA 
               
               
                 GFP 
                 AACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCCC 
               
               
                 ORF2- 
                 TTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAA 
               
               
                 C_Linker_ 
                 AGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC 
               
               
                 Nucleo- 
                 CTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCG 
               
               
                 plasmin- 
                 CGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGA 
               
               
                 NLS 
                 CTTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC 
               
               
                   
                 AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATTGGAGGACCGA 
               
               
                   
                 AGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGA 
               
               
                   
                 GCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACCTTG 
               
               
                   
                 CGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAGG 
               
               
                   
                 CGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATC 
               
               
                   
                 TGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT 
               
               
                   
                 ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGA 
               
               
                   
                 TAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTGA 
               
               
                   
                 TGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTT 
               
               
                   
                 GCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGGC 
               
               
                   
                 TCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCATC 
               
               
                   
                 GAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCAT 
               
               
                   
                 TAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAAA 
               
               
                   
                 AAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCAATCGTTGCGTTACACACACAA 
               
               
                   
                 AAAACCAACACACATCCATCTTCGATGGATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGCC 
               
               
                   
                 AGATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACT 
               
               
                   
                 TACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTAT 
               
               
                   
                 GTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTG 
               
               
                   
                 CCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAA 
               
               
                   
                 ATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTAC 
               
               
                   
                 GTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGT 
               
               
                   
                 TTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAA 
               
               
                   
                 TCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTA 
               
               
                   
                 CGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTAC 
               
               
                   
                 CATCCACTCGACACACCCGCCAGCGGCCGCTAATACGACTCACTATAGGGAGAAGTACTGCCACCAT 
               
               
                   
                 GGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGAG 
               
               
                   
                 CGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAGG 
               
               
                   
                 GATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGAA 
               
               
                   
                 TTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACTC 
               
               
                   
                 ATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGACC 
               
               
                   
                 AGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCAG 
               
               
                   
                 GGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAAC 
               
               
                   
                 CTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAAG 
               
               
                   
                 ACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAACG 
               
               
                   
                 CACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGTG 
               
               
                   
                 GAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCTA 
               
               
                   
                 TTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTAA 
               
               
                   
                 TATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGAG 
               
               
                   
                 GGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTCA 
               
               
                   
                 AGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGAACCACGC 
               
               
                   
                 AAAGATGTGAGACAGCCGTCAGACCATCAAGACTAGGAAGAAACTGCATCAACTAATGAGCAAAATC 
               
               
                   
                 ACCAGCTAACATCATAGTATACATGACCGGCTCTAACTCACATATCACCATCCTTACACTTAACATT 
               
               
                   
                 AACGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCG 
               
               
                   
                 TTTGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCG 
               
               
                   
                 AAAGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACG 
               
               
                   
                 GATTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATAC 
               
               
                   
                 AGCAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACA 
               
               
                   
                 GGTCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCA 
               
               
                   
                 TTGAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCAC 
               
               
                   
                 TGCATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTT 
               
               
                   
                 CAGCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAG 
               
               
                   
                 TGCAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAA 
               
               
                   
                 TCAAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTG 
               
               
                   
                 GGTCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACC 
               
               
                   
                 TATCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATA 
               
               
                   
                 AAAGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGA 
               
               
                   
                 ACAGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATC 
               
               
                   
                 GAAACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGA 
               
               
                   
                 TAGACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAA 
               
               
                   
                 CGACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCAT 
               
               
                   
                 TTGTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCAC 
               
               
                   
                 GGCTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAAT 
               
               
                   
                 TAACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTAT 
               
               
                   
                 ATGGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCA 
               
               
                   
                 ATTCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAA 
               
               
                   
                 CTTCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATC 
               
               
                   
                 CAACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGT 
               
               
                   
                 TTAACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGAT 
               
               
                   
                 CATCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAAC 
               
               
                   
                 AAACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACA 
               
               
                   
                 TTATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCT 
               
               
                   
                 CTCCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATT 
               
               
                   
                 AAAGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACC 
               
               
                   
                 TGGAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGG 
               
               
                   
                 CTATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAG 
               
               
                   
                 ATAATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACAC 
               
               
                   
                 GAGACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAA 
               
               
                   
                 TAAGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCT 
               
               
                   
                 AAAGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGA 
               
               
                   
                 AAACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAA 
               
               
                   
                 GAATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACA 
               
               
                   
                 GCCTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGC 
               
               
                   
                 CCCACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCT 
               
               
                   
                 CTTCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTT 
               
               
                   
                 ACACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGA 
               
               
                   
                 CACTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGAC 
               
               
                   
                 GCCCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGT 
               
               
                   
                 ACTGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTT 
               
               
                   
                 ATTCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAAC 
               
               
                   
                 GAATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCC 
               
               
                   
                 GCGAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCA 
               
               
                   
                 TGCGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCG 
               
               
                   
                 GGGCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGG 
               
               
                   
                 AAATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGC 
               
               
                   
                 TCGGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGC 
               
               
                   
                 GGCCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAG 
               
               
                   
                 AAAATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCG 
               
               
                   
                 TTGGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCA 
               
               
                   
                 TAGAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGggcggcggcagcaaa 
               
               
                   
                 aggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaagTAAAGCGCTTCTAGAAGTTGTC 
               
               
                   
                 TCCTCCTGCACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTA 
               
               
                   
                 CAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCT 
               
               
                   
                 GCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAAT 
               
               
                   
                 CCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGT 
               
               
                   
                 GTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTC 
               
               
                   
                 GCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGG 
               
               
                   
                 CTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCT 
               
               
                   
                 CGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCA 
               
               
                   
                 GCGGACCTTCCTTCCCGCtgagagacacaaaaaattccaacacactattgcaatgaaaataaatttc 
               
               
                   
                 ctttattagccagaagtcagatgctcaaggggcttcatgatgtccccataatttttggcagagggaa 
               
               
                   
                 aaagatctcagtggtatttgtgagccagggcattggccttctgataggcagcctgcacctgaggagt 
               
               
                   
                 gcggccgctttacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagc 
               
               
                   
                 aggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactgggtgctcaggtagtggt 
               
               
                   
                 tgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcac 
               
               
                   
                 gctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggcc 
               
               
                   
                 atgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctcct 
               
               
                   
                 tgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcg 
               
               
                   
                 ggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcg 
               
               
                   
                 gacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtca 
               
               
                   
                 gggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcag 
               
               
                   
                 cttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccg 
               
               
                   
                 tccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcgg 
               
               
                   
                 gatctgacggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccatt 
               
               
                   
                 tgcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaac 
               
               
                   
                 tcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccatt 
               
               
                   
                 gatgtactgccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagta 
               
               
                   
                 ggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaa 
               
               
                   
                 tagggggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactc 
               
               
                   
                 cacccattgacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtc 
               
               
                   
                 aatgggcgggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgGGCCTGC 
               
               
                   
                 TGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGC 
               
               
                   
                 CGCCTCCCCGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAG 
               
               
                   
                 ATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT 
               
               
                   
                 TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCA 
               
               
                   
                 TTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                 ATGTGGTATTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGA 
               
               
                   
                 GGGGTTTTTTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGAT 
               
               
                   
                 GCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTG 
               
               
                   
                 CCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGA 
               
               
                   
                 TCTCTCGATTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAG 
               
               
                   
                 TTCATCAAACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGA 
               
               
                   
                 TCTGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCG 
               
               
                   
                 CCCAGTTCCGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCG 
               
               
                   
                 CCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGG 
               
               
                   
                 AGGTAGCCAACATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCT 
               
               
                   
                 ATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCG 
               
               
                   
                 CAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGG 
               
               
                   
                 CAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGA 
               
               
                   
                 AGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCT 
               
               
                   
                 CCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCT 
               
               
                   
                 GCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGT 
               
               
                   
                 CGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAG 
               
               
                   
                 GCGCGGATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGG 
               
               
                   
                 TGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGA 
               
               
                   
                 CATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTG 
               
               
                   
                 CTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCT 
               
               
                   
                 AGTATGTAAGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTT 
               
               
                   
                 GACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTG 
               
               
                   
                 AGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACA 
               
               
                   
                 ATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCG 
               
               
                   
                 AGATCGACTTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCT 
               
               
                   
                 ATTGCACCCGTTCTCCGATTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAG 
               
               
                   
                 GAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA 
               
               
                   
                 AATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG 
               
               
                   
                 GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC 
               
               
                   
                 TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGC 
               
               
                   
                 TCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC 
               
               
                   
                 GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAG 
               
               
                   
                 CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGA 
               
               
                   
                 AGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT 
               
               
                   
                 GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG 
               
               
                   
                 AAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAAC 
               
               
                   
                 TCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAA 
               
               
                   
                 AATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT 
               
               
                   
                 CAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAAC 
               
               
                   
                 TCTCCAAGGCCCTCGTCGGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGA 
               
               
                   
                 ACGAACTATCGCAAGTCTCTTGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAG 
               
               
                   
                 GTATTACTCCAATCCCGAATATCCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAAT 
               
               
                   
                 CCCGATCTATCCGAGATCCGAGGAATATCGAAATCGGGGCGCGCCTGGTGTA (SEQ ID NO: 
               
               
                   
                 107) 
               
               
                   
               
               
                 RET- 
                 TGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAAT 
               
               
                 018 
                 ATCCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCG 
               
               
                 LINE1- 
                 AGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGAT 
               
               
                 GFP 
                 CCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGA 
               
               
                 ORF2- 
                 TATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTGATC 
               
               
                 NSV40 
                 GGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTT 
               
               
                 _NLS_ 
                 TCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT 
               
               
                 Linker 
                 TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTT 
               
               
                 ORF2- 
                 ACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAAT 
               
               
                 C_Nucleo- 
                 GATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA 
               
               
                 plasmin_ 
                 CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATC 
               
               
                 NLS 
                 TTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGC 
               
               
                   
                 CAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT 
               
               
                   
                 CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACA 
               
               
                   
                 CCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGC 
               
               
                   
                 TTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC 
               
               
                   
                 CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTG 
               
               
                   
                 CAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC 
               
               
                   
                 TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCGATT 
               
               
                   
                 CTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCC 
               
               
                   
                 AGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATT 
               
               
                   
                 TATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAA 
               
               
                   
                 CAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGG 
               
               
                   
                 CAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCA 
               
               
                   
                 TAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGG 
               
               
                   
                 TTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAGCGA 
               
               
                   
                 TTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTTCAT 
               
               
                   
                 AGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACG 
               
               
                   
                 ACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTG 
               
               
                   
                 ACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCA 
               
               
                   
                 AGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCT 
               
               
                   
                 TATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCGGTT 
               
               
                   
                 TTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATT 
               
               
                   
                 GACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCG 
               
               
                   
                 CCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGT 
               
               
                   
                 GAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTAATA 
               
               
                   
                 CGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCA 
               
               
                   
                 AGACACAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGAT 
               
               
                   
                 GGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAA 
               
               
                   
                 GATATCCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTA 
               
               
                   
                 TCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGA 
               
               
                   
                 GGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAG 
               
               
                   
                 ATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGC 
               
               
                   
                 AGGAGATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGA 
               
               
                   
                 AAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGG 
               
               
                   
                 CAAGCTAATGTGCAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGA 
               
               
                   
                 AAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTC 
               
               
                   
                 CAGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCT 
               
               
                   
                 CTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCT 
               
               
                   
                 GCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGC 
               
               
                   
                 AATAATAGATATCAACCCTTGCAGAACCACGCAAAGATGTGAGACAGCCGTCAGACCATCAAGACTA 
               
               
                   
                 GGAAGAAACTGCATCAACTAATGAGCAAAATCACCAGCTAACATCATAGTATACATGccaaagaaga 
               
               
                   
                 agcggaaggtcggcggcggcagcACCGGCTCTAACTCACATATCACCATCCTTACACTTAACATTAA 
               
               
                   
                 CGGCCTCAACTCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTT 
               
               
                   
                 TGTTGCATCCAAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAA 
               
               
                   
                 AGATTTATCAGGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGA 
               
               
                   
                 TTTCAAGCCCACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAG 
               
               
                   
                 CAGGAAGAACTTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGG 
               
               
                   
                 TCCTGTCCGATCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATT 
               
               
                   
                 GAGCACCCTGGATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTG 
               
               
                   
                 CATCAGGCAGATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCA 
               
               
                   
                 GCGCCCCACATCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTG 
               
               
                   
                 CAAGCGCACAGAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATC 
               
               
                   
                 AAGAACCTGACCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGG 
               
               
                   
                 TCCACAATGAGATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTA 
               
               
                   
                 TCAAAACCTTTGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAA 
               
               
                   
                 AGAAAACAAGAGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAAC 
               
               
                   
                 AGACCCACTCCAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGA 
               
               
                   
                 AACCCAAAAGACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATA 
               
               
                   
                 GACAGACCTCTGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACG 
               
               
                   
                 ACAAGGGCGACATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTT 
               
               
                   
                 GTATGCTAACAAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGG 
               
               
                   
                 CTTAATCAAGAGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTA 
               
               
                   
                 ACTCCCTGCCGACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATAT 
               
               
                   
                 GGAAGAGCTTGTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAAT 
               
               
                   
                 TCCTTCTACGAAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACT 
               
               
                   
                 TCCGGCCCATTAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCA 
               
               
                   
                 ACAACATATTAAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTT 
               
               
                   
                 AACATCCGGAAGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGATCA 
               
               
                   
                 TCTCTATAGACGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAA 
               
               
                   
                 ACTCGGCATCGACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATT 
               
               
                   
                 ATCCTTAACGGCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCT 
               
               
                   
                 CCCCGCTTTTGTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAA 
               
               
                   
                 AGGGATACAGCTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTG 
               
               
                   
                 GAGAATCCTATTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCT 
               
               
                   
                 ATAAGATTAACGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGAT 
               
               
                   
                 AATGGGTGAGCTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGA 
               
               
                   
                 GACGTTAAAGATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATA 
               
               
                   
                 AGTGGAAGAATATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAA 
               
               
                   
                 AGTGATATATCGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAA 
               
               
                   
                 ACAACCCTTAAATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGA 
               
               
                   
                 ATAAGGCCGGTGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGC 
               
               
                   
                 CTGGTATTGGTATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGCCC 
               
               
                   
                 CACATCTATAATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCT 
               
               
                   
                 TCAACAAGTGGTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTAC 
               
               
                   
                 ACCCTACACTAAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACA 
               
               
                   
                 CTGGAAGAGAATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGC 
               
               
                   
                 CCAAGGCCATGGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTAC 
               
               
                   
                 TGCCAAGGAGACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTAT 
               
               
                   
                 TCATCAGATAAGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGA 
               
               
                   
                 ATAATCCCATCAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGC 
               
               
                   
                 GAAGAAGCATATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATG 
               
               
                   
                 CGATACCACCTTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGG 
               
               
                   
                 GCTGTGGCGAGATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAA 
               
               
                   
                 ATCAGTCTGGCGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTC 
               
               
                   
                 GGAATCTATCCTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGG 
               
               
                   
                 CCTTGTTTACGATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAA 
               
               
                   
                 AATGTGGCATATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTT 
               
               
                   
                 GGGACCTGGATGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATA 
               
               
                   
                 GAATCTTCTCTCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGaaaaggccggcggccac 
               
               
                   
                 gaaaaaggccggccaggcaaaaaagaaaaagTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGA 
               
               
                   
                 CTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAA 
               
               
                   
                 GATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTT 
               
               
                   
                 GTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCT 
               
               
                   
                 CTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAA 
               
               
                   
                 CCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCC 
               
               
                   
                 TATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGC 
               
               
                   
                 ACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCA 
               
               
                   
                 CCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTC 
               
               
                   
                 CCGCtgagagacacaaaaaattccaacacactattgcaatgaaaataaatttcctttattagccaga 
               
               
                   
                 agtcagatgctcaaggggcttcatgatgtccccataatttttggcagagggaaaaagatctcagtgg 
               
               
                   
                 tatttgtgagccagggcattggccttctgataggcagcctgcacctgaggagtgcggccgctttact 
               
               
                   
                 tgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatc 
               
               
                   
                 gcgcttctcgttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagcagc 
               
               
                   
                 acggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcga 
               
               
                   
                 tgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgtt 
               
               
                   
                 gtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgccc 
               
               
                   
                 ttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgc 
               
               
                   
                 cgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtc 
               
               
                   
                 gtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagg 
               
               
                   
                 gtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtgg 
               
               
                   
                 catcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccag 
               
               
                   
                 gatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcgggatctgacggttca 
               
               
                   
                 ctaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggc 
               
               
                   
                 ggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtca 
               
               
                   
                 atggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaa 
               
               
                   
                 accgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataa 
               
               
                   
                 ggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtactt 
               
               
                   
                 ggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtc 
               
               
                   
                 aatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtc 
               
               
                   
                 gttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGG 
               
               
                   
                 CCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTG 
               
               
                   
                 TCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGATGAGT 
               
               
                   
                 TTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGC 
               
               
                   
                 TTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT 
               
               
                   
                 CAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCC 
               
               
                   
                 CATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGC 
               
               
                   
                 CCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGT 
               
               
                   
                 CTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACG 
               
               
                   
                 TGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGA 
               
               
                   
                 TCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTC 
               
               
                   
                 TTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATT 
               
               
                   
                 AGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCA 
               
               
                   
                 TTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAG 
               
               
                   
                 CTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATG 
               
               
                   
                 ATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACT 
               
               
                   
                 GGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGT 
               
               
                   
                 TCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCG 
               
               
                   
                 TGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACT 
               
               
                   
                 GGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGT 
               
               
                   
                 ATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCAC 
               
               
                   
                 CAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATC 
               
               
                   
                 TGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGA 
               
               
                   
                 CGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGC 
               
               
                   
                 TTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTA 
               
               
                   
                 CCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGC 
               
               
                   
                 CGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCT 
               
               
                   
                 GTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTG 
               
               
                   
                 CCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTC 
               
               
                   
                 TATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCT 
               
               
                   
                 GGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCT 
               
               
                   
                 GGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCT 
               
               
                   
                 CCGATTAGGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG 
               
               
                   
                 CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAG 
               
               
                   
                 TCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTG 
               
               
                   
                 CGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG 
               
               
                   
                 CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTG 
               
               
                   
                 TGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAAC 
               
               
                   
                 CCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG 
               
               
                   
                 TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGG 
               
               
                   
                 TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAA 
               
               
                   
                 ACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTC 
               
               
                   
                 AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT 
               
               
                   
                 TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAA 
               
               
                   
                 TCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTA 
               
               
                   
                 TCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTC 
               
               
                   
                 GTCGGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAA 
               
               
                   
                 GTCTCT (SEQ ID NO: 108) 
               
               
                   
               
               
                 ret-028- 
                 TGGCCGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAAT 
               
               
                 linel- 
                 ATCCGAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCG 
               
               
                 orf1-t2a- 
                 AGGAATATCGAAATCGGGGCGCGCCTGGTGTACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGAT 
               
               
                 orf2- 
                 CCATATCGTTGCTTGGCAGTCAGCCAGTCGGAATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGA 
               
               
                 n_sv40_ 
                 TATTGTACTCAAGCCTGGTCACGGCAGCGTACCGATCTGTTTAAACCTAGATATTGATAGTCTGATC 
               
               
                 nls_linker- 
                 GGTCAACGTATAATCGAGTCCTAGCTTTTGCAAACATCTATCAAGAGACAGGATCAGCAGGAGGCTT 
               
               
                 gfp 
                 TCGCATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT 
               
               
                   
                 TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTT 
               
               
                   
                 ACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAAT 
               
               
                   
                 GATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA 
               
               
                   
                 CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATC 
               
               
                   
                 TTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGC 
               
               
                   
                 CAACTTACTTCTGACAACGATTGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT 
               
               
                   
                 CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACA 
               
               
                   
                 CCACGATGCCTGTAGCAATGGCAACAACCTTGCGTAAACTATTAACTGGCGAACTACTTACTCTAGC 
               
               
                   
                 TTCCCGGCAACAGTTGATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC 
               
               
                   
                 CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTG 
               
               
                   
                 CAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC 
               
               
                   
                 TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACCGATT 
               
               
                   
                 CTAGGTGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCC 
               
               
                   
                 AGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATT 
               
               
                   
                 TATCCTTAAGATCGTTTAAACTCGACTCTGGCTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAA 
               
               
                   
                 CAGCCGTGGCGCTCATTTGCTCGTCGGGCATCGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGG 
               
               
                   
                 CAGCGATCGCGGCTCCCGACATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCA 
               
               
                   
                 TAACAGCAGCTTCAGCTACCTCTCAATTCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGG 
               
               
                   
                 TTATGCTATCAATCGTTGCGTTACACACACAAAAAACCAACACACATCCATCTTCGATGGATAGCGA 
               
               
                   
                 TTTTATTATCTAACTGCTGATCGAGTGTAGCCAGATCTAGTAATCAATTACGGGGTCATTAGTTCAT 
               
               
                   
                 AGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACG 
               
               
                   
                 ACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTG 
               
               
                   
                 ACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCA 
               
               
                   
                 AGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCT 
               
               
                   
                 TATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGCTGATGCGGTT 
               
               
                   
                 TTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATT 
               
               
                   
                 GACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCG 
               
               
                   
                 CCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGT 
               
               
                   
                 GAACCGTCAGATCAGATCTTTGTCGATCCTACCATCCACTCGACACACCCGCCAGCGGCCGCTAATA 
               
               
                   
                 CGACTCACTATAGGGAGAAGTACTGCCACCATGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCA 
               
               
                   
                 AGACACAATCCGCTAGCCCACCACCTAAAGAGCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGAT 
               
               
                   
                 GGAAAACGACTTCGATGAACTCCGGGAAGAGGGATTTAGGCGATCCAACTATTCAGAACTCCGCGAA 
               
               
                   
                 GATATCCAGACAAAGGGGAAGGAAGTCGAGAATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTA 
               
               
                   
                 TCACAAACACTGAGAAATGTCTCAAAGAACTCATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGA 
               
               
                   
                 GGAGTGTCGGAGTCTGAGATCCAGGTGTGACCAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAG 
               
               
                   
                 ATGAACGAGATGAAAAGAGAGGGCAAATTCAGGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGC 
               
               
                   
                 AGGAGATTTGGGATTACGTCAAGAGGCCTAACCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGA 
               
               
                   
                 AAACGGGACTAAACTGGAGAATACACTTCAAGACATCATTCAAGAAAATTTTCCAAACCTGGCTCGG 
               
               
                   
                 CAAGCTAATGTGCAAATCCAAGAGATCCAACGCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCC 
               
               
                   
                 CTAGGCATATTATCGTGCGCTTTACTAAGGTGGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGA 
               
               
                   
                 AAAGGGAAGGGTGACTTTGAAGGGCAAACCTATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTC 
               
               
                   
                 CAGGCACGCCGGGAATGGGGCCCCATCTTTAATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCT 
               
               
                   
                 CTTACCCTGCAAAGTTGAGTTTTATCTCCGAGGGTGAGATTAAGTATTTCATCGATAAACAGATGCT 
               
               
                   
                 GCGAGACTTCGTGACAACTCGCCCAGCTCTCAAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGC 
               
               
                   
                 AATAATAGATATCAACCCTTGCAGAACCACGCAAAGATGggctccggcgagggcaggggaagCcttc 
               
               
                   
                 taacatgcggggacgtggaggaaaatcccggcccaGGTAGCGGCccaaagaagaagcggaaggtcgg 
               
               
                   
                 cggcggcagcACCGGCTCTAACTCACATATCACCATCCTTACACTTAACATTAACGGCCTCAACTCA 
               
               
                   
                 GCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCCAAG 
               
               
                   
                 AGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTTATCAGGC 
               
               
                   
                 GAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCCACC 
               
               
                   
                 AAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAGAACTTA 
               
               
                   
                 CCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGATCT 
               
               
                   
                 GCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATTGAGCACCCTGGAT 
               
               
                   
                 CGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAGATC 
               
               
                   
                 TCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCACATCA 
               
               
                   
                 TACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACAGAG 
               
               
                   
                 ATAATTACAAATTAGCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGACCC 
               
               
                   
                 AGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGGTCCACAATGAGAT 
               
               
                   
                 GAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTATCAAAACCTTTGG 
               
               
                   
                 GATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAAAGAAAACAAGAGA 
               
               
                   
                 GATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTCCAA 
               
               
                   
                 GGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGAAACCCAAAAGACT 
               
               
                   
                 CTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAGACCTCTGG 
               
               
                   
                 CACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACGACAAGGGCGACAT 
               
               
                   
                 CACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTGTATGCTAACAAG 
               
               
                   
                 CTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGGCTTAATCAAGAGG 
               
               
                   
                 AAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCCCTGCCGAC 
               
               
                   
                 AAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATATGGAAGAGCTTGTA 
               
               
                   
                 CCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTACGAAG 
               
               
                   
                 CTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACTTCCGGCCCATTAG 
               
               
                   
                 TCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACATATTAAG 
               
               
                   
                 AAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTAACATCCGGAAGA 
               
               
                   
                 GTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTATAGACGC 
               
               
                   
                 GGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAAACTCGGCATCGAC 
               
               
                   
                 GGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTTAACGGCC 
               
               
                   
                 AAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTCCCCGCTTTTGTT 
               
               
                   
                 TAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATACAGCTC 
               
               
                   
                 GGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGGAGAATCCTATTG 
               
               
                   
                 TGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATTAACGT 
               
               
                   
                 CCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATAATGGGTGAGCTT 
               
               
                   
                 CCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAAGATT 
               
               
                   
                 TGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGTGGAAGAATAT 
               
               
                   
                 CCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATATCGC 
               
               
                   
                 TTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAACCCTTAAAT 
               
               
                   
                 TTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGGTGG 
               
               
                   
                 GATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGTATTGGTAT 
               
               
                   
                 CAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATAATT 
               
               
                   
                 ACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACAAGTGGTG 
               
               
                   
                 TTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACTAAA 
               
               
                   
                 ATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAAGAGAATC 
               
               
                   
                 TTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCATGGC 
               
               
                   
                 CACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAAGGAGACC 
               
               
                   
                 ACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATAAGG 
               
               
                   
                 GGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATCCCATCAA 
               
               
                   
                 GAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCATATG 
               
               
                   
                 AAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATACCACCTTA 
               
               
                   
                 CCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGAGAT 
               
               
                   
                 TGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGGCGC 
               
               
                   
                 TTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATCCTA 
               
               
                   
                 ACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTACGAT 
               
               
                   
                 AGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAAAATGTGGCATATT 
               
               
                   
                 TATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGATGA 
               
               
                   
                 AGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTCTCT 
               
               
                   
                 CATTGGTGGTAACGACTACAAAGACGATGACGACAAGTAAAGCGCTTCTAGAAGTTGTCTCCTCCTG 
               
               
                   
                 CACTGACTGACTGATACAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTT 
               
               
                   
                 GTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAAT 
               
               
                   
                 GCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTG 
               
               
                   
                 CTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTG 
               
               
                   
                 ACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCC 
               
               
                   
                 CCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTG 
               
               
                   
                 TTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTG 
               
               
                   
                 TTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCT 
               
               
                   
                 TCCTTCCCGCtgagagacacaaaaaattccaacacactattgcaatgaaaataaatttcctttatta 
               
               
                   
                 gccagaagtcagatgctcaaggggcttcatgatgtccccataatttttggcagagggaaaaagatct 
               
               
                   
                 cagtggtatttgtgagccagggcattggccttctgataggcagcctgcacctgaggagtgcggccgc 
               
               
                   
                 tttacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccat 
               
               
                   
                 gtgatcgcgcttctcgttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggc 
               
               
                   
                 agcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgt 
               
               
                   
                 cctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatata 
               
               
                   
                 gacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcg 
               
               
                   
                 atgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgt 
               
               
                   
                 agttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaa 
               
               
                   
                 gaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtc 
               
               
                   
                 acgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgt 
               
               
                   
                 aggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctc 
               
               
                   
                 gaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcgggatctgac 
               
               
                   
                 ggttcactaaaccagctctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaa 
               
               
                   
                 tggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattg 
               
               
                   
                 acgtcaatggggtggagacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtact 
               
               
                   
                 gccaaaaccgcatcaccatggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtc 
               
               
                   
                 ccataaggtcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggc 
               
               
                   
                 gtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccatt 
               
               
                   
                 gacgtcaatggaaagtccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcg 
               
               
                   
                 ggggtcgttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgGGCCTGCTGCCGGCT 
               
               
                   
                 CTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCC 
               
               
                   
                 CGCCTGTCTAGCTTGACTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTG 
               
               
                   
                 ATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGC 
               
               
                   
                 TATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT 
               
               
                   
                 ATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTA 
               
               
                   
                 TTGGCCCATCTCTATCGGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTT 
               
               
                   
                 TTGTGCCCCTCGGGCCGGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCT 
               
               
                   
                 GCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTC 
               
               
                   
                 CATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGA 
               
               
                   
                 TTTCGATCAAGACATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAA 
               
               
                   
                 ACTTTCTTCCCTCCCTAATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATC 
               
               
                   
                 TCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTC 
               
               
                   
                 CGCCCATTCTCCGCCCCATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCC 
               
               
                   
                 TCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCC 
               
               
                   
                 AACATGATTGAACAAGATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCT 
               
               
                   
                 ATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCG 
               
               
                   
                 CCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGG 
               
               
                   
                 CTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAA 
               
               
                   
                 GGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGA 
               
               
                   
                 GAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTC 
               
               
                   
                 GACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGG 
               
               
                   
                 ATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGAT 
               
               
                   
                 GCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAAT 
               
               
                   
                 GGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGT 
               
               
                   
                 TGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGG 
               
               
                   
                 TATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTA 
               
               
                   
                 AGCCCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGG 
               
               
                   
                 AAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTG 
               
               
                   
                 TCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGG 
               
               
                   
                 CATGCTGGGGATGCGGTGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGAC 
               
               
                   
                 TTGTCTGGGTTTCGACTACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACC 
               
               
                   
                 CGTTCTCCGATTACGAGTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA 
               
               
                   
                 AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACG 
               
               
                   
                 CTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCC 
               
               
                   
                 CTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA 
               
               
                   
                 GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCT 
               
               
                   
                 GGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG 
               
               
                   
                 TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA 
               
               
                   
                 GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGT 
               
               
                   
                 ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGC 
               
               
                   
                 AAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG 
               
               
                   
                 GATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTA 
               
               
                   
                 AGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGT 
               
               
                   
                 TTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGG 
               
               
                   
                 CACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAACTCTCCAAG 
               
               
                   
                 GCCCTCGTCGGAAAATCTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTA 
               
               
                   
                 TCGCAAGTCTCT (SEQ ID NO: 109) 
               
               
                   
               
               
                 ret-029- 
                 ACCGAGAACGATCCTCTCAGTGCGAGTCTCGACGATCCATATCGTTGCTTGGCAGTCAGCCAGTCGG 
               
               
                 line1- 
                 AATCCAGCTTGGGACCCAGGAAGTCCAATCGTCAGATATTGTACTCAAGCCTGGTCACGGCAGCGTA 
               
               
                 orf1-t2a- 
                 CCGATCTGTTTAAACCTAGATATTGATAGTCTGATCGGTCAACGTATAATCGAGTCCTAGCTTTTGC 
               
               
                 orf- 
                 AAACATCTATCAAGAGACAGGATCAGCAGGAGGCTTTCGCATGAGTATTCAACATTTCCGTGTCGCC 
               
               
                 c_nucleo- 
                 CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAA 
               
               
                 plasmin_ 
                 AAGATGCTGAAGATCAGTTGGGTGCGCGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGAT 
               
               
                 nls-gfp 
                 CCTTGAGAGTTTTCGCCCCGAAGAACGCTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGC 
               
               
                   
                 GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATG 
               
               
                   
                 ACTTGGTTGAGTATTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATG 
               
               
                   
                 CAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATTGGAGGACCG 
               
               
                   
                 AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG 
               
               
                   
                 AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACCTT 
               
               
                   
                 GCGTAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAGTTGATAGACTGGATGGAG 
               
               
                   
                 GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAAT 
               
               
                   
                 CTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCG 
               
               
                   
                 TATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG 
               
               
                   
                 ATAGGTGCCTCACTGATTAAGCATTGGTAACCGATTCTAGGTGCATTGGCGCAGAAAAAAATGCCTG 
               
               
                   
                 ATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACT 
               
               
                   
                 TGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAAGATCGTTTAAACTCGACTCTGG 
               
               
                   
                 CTCTATCGAATCTCCGTCGTTTCGAGCTTACGCGAACAGCCGTGGCGCTCATTTGCTCGTCGGGCAT 
               
               
                   
                 CGAATCTCGTCAGCTATCGTCAGCTTACCTTTTTGGCAGCGATCGCGGCTCCCGACATCTTGGACCA 
               
               
                   
                 TTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCTTCAGCTACCTCTCAATTCAA 
               
               
                   
                 AAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTATCAATCGTTGCGTTACACACACA 
               
               
                   
                 AAAAACCAACACACATCCATCTTCGATGGATAGCGATTTTATTATCTAACTGCTGATCGAGTGTAGC 
               
               
                   
                 CAGATCTAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAAC 
               
               
                   
                 TTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTA 
               
               
                   
                 TGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACT 
               
               
                   
                 GCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTA 
               
               
                   
                 AATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTA 
               
               
                   
                 CGTATTAGTCATCGCTATTACCATGCTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGG 
               
               
                   
                 TTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAA 
               
               
                   
                 ATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGT 
               
               
                   
                 ACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCAGATCTTTGTCGATCCTA 
               
               
                   
                 CCATCCACTCGACACACCCGCCAGCGGCCGCTAATACGACTCACTATAGGGAGAAGTACTGCCACCA 
               
               
                   
                 TGGGCAAGAAGCAAAATCGCAAGACGGGGAATTCCAAGACACAATCCGCTAGCCCACCACCTAAAGA 
               
               
                   
                 GCGTTCTAGCTCCCCTGCTACTGAGCAGTCCTGGATGGAAAACGACTTCGATGAACTCCGGGAAGAG 
               
               
                   
                 GGATTTAGGCGATCCAACTATTCAGAACTCCGCGAAGATATCCAGACAAAGGGGAAGGAAGTCGAGA 
               
               
                   
                 ATTTCGAGAAGAACCTCGAGGAGTGCATCACCCGTATCACAAACACTGAGAAATGTCTCAAAGAACT 
               
               
                   
                 CATGGAACTTAAGACAAAAGCCAGGGAGCTTCGAGAGGAGTGTCGGAGTCTGAGATCCAGGTGTGAC 
               
               
                   
                 CAGCTCGAGGAGCGCGTGAGCGCGATGGAAGACGAGATGAACGAGATGAAAAGAGAGGGCAAATTCA 
               
               
                   
                 GGGAGAAGCGCATTAAGAGGAACGAACAGAGTCTGCAGGAGATTTGGGATTACGTCAAGAGGCCTAA 
               
               
                   
                 CCTGCGGTTGATCGGCGTCCCCGAGAGCGACGTAGAAAACGGGACTAAACTGGAGAATACACTTCAA 
               
               
                   
                 GACATCATTCAAGAAAATTTTCCAAACCTGGCTCGGCAAGCTAATGTGCAAATCCAAGAGATCCAAC 
               
               
                   
                 GCACACCCCAGCGGTATAGCTCTCGGCGTGCCACCCCTAGGCATATTATCGTGCGCTTTACTAAGGT 
               
               
                   
                 GGAGATGAAAGAGAAGATGCTGCGAGCCGCTCGGGAAAAGGGAAGGGTGACTTTGAAGGGCAAACCT 
               
               
                   
                 ATTCGGCTGACGGTTGACCTTAGCGCCGAGACACTCCAGGCACGCCGGGAATGGGGCCCCATCTTTA 
               
               
                   
                 ATATCCTGAAGGAGAAGAACTTCCAGCCACGAATCTCTTACCCTGCAAAGTTGAGTTTTATCTCCGA 
               
               
                   
                 GGGTGAGATTAAGTATTTCATCGATAAACAGATGCTGCGAGACTTCGTGACAACTCGCCCAGCTCTC 
               
               
                   
                 AAGGAACTGCTCAAAGAGGCTCTTAATATGGAGCGCAATAATAGATATCAACCCTTGCAGAACCACG 
               
               
                   
                 CAAAGATGggctccggcgagggcaggggaagCcttctaacatgcggggacgtggaggaaaatcccgg 
               
               
                   
                 cccaGGTAGCGGCACCGGCTCTAACTCACATATCACCATCCTTACACTTAACATTAACGGCCTCAAC 
               
               
                   
                 TCAGCTATCAAGCGCCATCGGCTGGCCAGCTGGATCAAATCACAGGATCCAAGCGTTTGTTGCATCC 
               
               
                   
                 AAGAGACCCACCTGACCTGTAGAGATACTCACCGCCTCAAGATCAAGGGATGGCGAAAGATTTATCA 
               
               
                   
                 GGCGAACGGTAAGCAGAAGAAAGCCGGAGTCGCAATTCTGGTCTCAGACAAGACGGATTTCAAGCCC 
               
               
                   
                 ACCAAAATTAAGCGTGATAAGGAAGGTCACTATATTATGGTGAAAGGCAGCATACAGCAGGAAGAAC 
               
               
                   
                 TTACCATATTGAACATCTACGCGCCAAACACCGGCGCACCTCGCTTTATCAAACAGGTCCTGTCCGA 
               
               
                   
                 TCTGCAGCGAGATCTGGATTCTCATACGTTGATTATGGGTGATTTCAATACACCATTGAGCACCCTG 
               
               
                   
                 GATCGCAGCACCAGGCAAAAGGTAAATAAAGACACGCAAGAGCTCAATAGCGCACTGCATCAGGCAG 
               
               
                   
                 ATCTCATTGATATTTATCGCACTCTTCATCCTAAGAGTACCGAGTACACATTCTTCAGCGCCCCACA 
               
               
                   
                 TCATACATACTCAAAGATCGATCATATCGTCGGCTCAAAGGCTCTGCTGTCAAAGTGCAAGCGCACA 
               
               
                   
                 GAGATAATTACAAATTACCTGTCAGATCATAGCGCGATCAAGCTCGAGCTGAGAATCAAGAACCTGA 
               
               
                   
                 CCCAGAGCCGGAGTACCACTTGGAAGCTTAATAACCTGCTGCTCAACGATTATTGGGTCCACAATGA 
               
               
                   
                 GATGAAGGCAGAGATTAAAATGTTCTTCGAAACAAATGAGAATAAGGATACTACCTATCAAAACCTT 
               
               
                   
                 TGGGATGCCTTTAAGGCCGTCTGCAGAGGCAAGTTCATCGCCCTCAACGCCTATAAAAGAAAACAAG 
               
               
                   
                 AGAGATCTAAGATCGATACTCTCACCTCTCAGCTGAAGGAGTTGGAGAAACAGGAACAGACCCACTC 
               
               
                   
                 CAAGGCGTCAAGACGGCAGGAGATCACAAAGATTCGCGCCGAGTTGAAAGAGATCGAAACCCAAAAG 
               
               
                   
                 ACTCTTCAGAAAATTAACGAGTCTCGTAGTTGGTTCTTCGAGCGGATTAATAAGATAGACAGACCTC 
               
               
                   
                 TGGCACGACTGATTAAGAAGAAGCGCGAAAAGAACCAGATTGATACCATCAAGAACGACAAGGGCGA 
               
               
                   
                 CATCACTACTGACCCGACCGAGATCCAGACCACTATTCGGGAGTATTATAAGCATTTGTATGCTAAC 
               
               
                   
                 AAGCTTGAGAACCTGGAAGAGATGGACACTTTTCTGGATACCTATACTCTGCCACGGCTTAATCAAG 
               
               
                   
                 AGGAAGTCGAGTCCCTCAACCGCCCAATTACAGGAAGCGAGATTGTGGCCATAATTAACTCCCTGCC 
               
               
                   
                 GACAAAGAAATCTCCTGGTCCGGACGGGTTTACAGCTGAGTTTTATCAACGGTATATGGAAGAGCTT 
               
               
                   
                 GTACCGTTTCTGCTCAAGCTCTTTCAGTCTATAGAAAAGGAAGGCATCTTGCCCAATTCCTTCTACG 
               
               
                   
                 AAGCTTCTATAATACTTATTCCCAAACCAGGACGCGATACCACAAAGAAGGAAAACTTCCGGCCCAT 
               
               
                   
                 TAGTCTCATGAATATCGACGCTAAAATATTGAACAAGATTCTCGCCAACAGAATCCAACAACATATT 
               
               
                   
                 AAGAAATTGATACATCACGACCAGGTGGGGTTTATACCTGGCATGCAGGGCTGGTTTAACATCCGGA 
               
               
                   
                 AGAGTATTAACGTCATTCAACACATTAATAGAGCTAAGGATAAGAATCATATGATCATCTCTATAGA 
               
               
                   
                 CGCGGAAAAGGCATTCGATAAGATTCAGCAGCCATTTATGCTCAAGACTCTGAACAAACTCGGCATC 
               
               
                   
                 GACGGAACATATTTTAAGATTATTCGCGCAATTTACGATAAGCCGACTGCTAACATTATCCTTAACG 
               
               
                   
                 GCCAAAAGCTCGAGGCCTTTCCGCTCAAGACTGGAACCCGCCAAGGCTGTCCCCTCTCCCCGCTTTT 
               
               
                   
                 GTTTAATATTGTACTCGAGGTGCTGGCTAGGGCTATTCGTCAAGAGAAAGAGATTAAAGGGATACAG 
               
               
                   
                 CTCGGGAAGGAAGAGGTCAAGCTTTCCTTGTTCGCCGATGATATGATTGTGTACCTGGAGAATCCTA 
               
               
                   
                 TTGTGTCTGCTCAGAACCTTCTTAAACTTATTTCTAACTTTAGCAAGGTCAGCGGCTATAAGATTAA 
               
               
                   
                 CGTCCAGAAATCTCAGGCCTTTCTGTACACAAATAATCGACAGACCGAATCCCAGATAATGGGTGAG 
               
               
                   
                 CTTCCGTTTGTCATAGCCAGCAAAAGGATAAAGTATCTCGGAATCCAGCTGACACGAGACGTTAAAG 
               
               
                   
                 ATTTGTTTAAGGAAAATTACAAGCCTCTCCTGAAAGAGATTAAGGAAGATACTAATAAGTGGAAGAA 
               
               
                   
                 TATCCCCTGTTCATGGGTTGGCAGAATCAACATAGTGAAGATGGCAATACTTCCTAAAGTGATATAT 
               
               
                   
                 CGCTTTAACGCCATCCCAATTAAACTGCCTATGACCTTCTTTACGGAGCTCGAGAAAACAACCCTTA 
               
               
                   
                 AATTTATATGGAATCAAAAGAGAGCAAGAATAGCGAAGTCCATCTTGAGCCAGAAGAATAAGGCCGG 
               
               
                   
                 TGGGATTACTTTGCCTGATTTTAAGTTGTATTATAAAGCCACAGTAACTAAGACAGCCTGGTATTGG 
               
               
                   
                 TATCAGAATAGAGACATCGACCAGTGGAATCGGACCGAACCATCAGAGATAATGCCCCACATCTATA 
               
               
                   
                 ATTACCTTATATTCGATAAGCCAGAAAAGAATAAACAGTGGGGCAAAGACAGCCTCTTCAACAAGTG 
               
               
                   
                 GTGTTGGGAGAATTGGCTGGCCATATGCCGGAAACTCAAGCTCGACCCCTTTCTTACACCCTACACT 
               
               
                   
                 AAAATCAACAGTAGGTGGATCAAGGACTTGAATGTCAAGCCAAAGACTATAAAGACACTGGAAGAGA 
               
               
                   
                 ATCTTGGGATCACAATACAAGATATAGGCGTCGGCAAAGATTTTATGTCAAAGACGCCCAAGGCCAT 
               
               
                   
                 GGCCACTAAGGATAAGATTGATAAGTGGGACCTTATTAAGCTCAAAAGCTTCTGTACTGCCAAGGAG 
               
               
                   
                 ACCACGATCAGAGTTAATAGGCAGCCCACTACATGGGAAAAGATTTTCGCCACTTATTCATCAGATA 
               
               
                   
                 AGGGGTTGATAAGCAGAATATATAACGAGCTGAAGCAGATCTACAAGAAGAAAACGAATAATCCCAT 
               
               
                   
                 CAAGAAGTGGGCAAAAGATATGAACAGGCATTTTAGCAAAGAGGATATCTACGCCGCGAAGAAGCAT 
               
               
                   
                 ATGAAGAAGTGTAGTTCAAGCTTGGCCATTCGTGAGATGCAGATTAAGACGACCATGCGATACCACC 
               
               
                   
                 TTACCCCAGTGAGGATGGCAATTATCAAGAAATCTGGCAATAATAGATGTTGGCGGGGCTGTGGCGA 
               
               
                   
                 GATTGGCACCCTGCTCCATTGCTGGTGGGATTGCAAGCTGGTGCAGCCGCTTTGGAAATCAGTCTGG 
               
               
                   
                 CGCTTTCTGAGGGACCTCGAGCTTGAGATTCCCTTCGATCCCGCAATTCCCTTGCTCGGAATCTATC 
               
               
                   
                 CTAACGAATACAAGAGCTGTTGTTACAAGGATACGTGTACCCGGATGTTCATCGCGGCCTTGTTTAC 
               
               
                   
                 GATAGCTAAGACGTGGAATCAGCCTAAGTGCCCCACAATGATCGATTGGATCAAGAAAATGTGGCAT 
               
               
                   
                 ATTTATACCATGGAGTATTACGCAGCAATTAAGAATGACGAATTTATTTCCTTCGTTGGGACCTGGA 
               
               
                   
                 TGAAGCTGGAGACTATTATTCTGAGCAAGCTGTCTCAGGAGCAAAAGACAAAGCATAGAATCTTCTC 
               
               
                   
                 TCTCATTGGTGGTAACGACTACAAAGACGATGACGACAAGaaaaggccggcggccacgaaaaaggcc 
               
               
                   
                 ggccaggcaaaaaagaaaaagTAAAGCGCTTCTAGAAGTTGTCTCCTCCTGCACTGACTGACTGATA 
               
               
                   
                 CAATCGATTTCTGGATCCGCAGGCCTAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGG 
               
               
                   
                 TATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCT 
               
               
                   
                 ATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGG 
               
               
                   
                 AGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGG 
               
               
                   
                 TTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACG 
               
               
                   
                 GCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATT 
               
               
                   
                 CCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCT 
               
               
                   
                 GCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCtgagag 
               
               
                   
                 acacaaaaaattccaacacactattgcaatgaaaataaatttcctttattagccagaagtcagatgc 
               
               
                   
                 tcaaggggcttcatgatgtccccataatttttggcagagggaaaaagatctcagtggtatttgtgag 
               
               
                   
                 ccagggcattggccttctgataggcagcctgcacctgaggagtgcggccgctttacttgtacagctc 
               
               
                   
                 gtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcg 
               
               
                   
                 ttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgt 
               
               
                   
                 cgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcg 
               
               
                   
                 gatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttg 
               
               
                   
                 tagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcga 
               
               
                   
                 tgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtcctt 
               
               
                   
                 gaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttc 
               
               
                   
                 atgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagg 
               
               
                   
                 gcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctc 
               
               
                   
                 gccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcacc 
               
               
                   
                 accccggtgaacagctcctcgcccttgctcaccatggtggcgggatctgacggttcactaaaccagc 
               
               
                   
                 tctgcttatatagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcggagttgtta 
               
               
                   
                 cgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattgacgtcaatggggtgga 
               
               
                   
                 gacttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcac 
               
               
                   
                 catggtaatagcgatgactaatacgtagatgtactgccaagtaggaaagtcccataaggtcatgtac 
               
               
                   
                 tgggcataatgccaggcgggccatttaccgtcattgacgtcaatagggggcgtacttggcatatgat 
               
               
                   
                 acacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgacgtcaatggaaagt 
               
               
                   
                 ccctattggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggt 
               
               
                   
                 cagccaggcgggccatttaccgtaagttatgtaacgGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGC 
               
               
                   
                 GTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCTAGCTTGA 
               
               
                   
                 CTGACTGAGATACAGCGTACCTTCAGCTCACAGACATGATAAGATACATTGATGAGTTTGGACAAAC 
               
               
                   
                 CACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA 
               
               
                   
                 ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGG 
               
               
                   
                 GGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATTGGCCCATCTCTATC 
               
               
                   
                 GGTATCGTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCCCCTCGGGCC 
               
               
                   
                 GGATTGCTATCTACCGGCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCC 
               
               
                   
                 CACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTT 
               
               
                   
                 ACCAGAAATTTATCCTTAAGGTCGTCAGCTATCCTGCAGGCGATCTCTCGATTTCGATCAAGACATT 
               
               
                   
                 CCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCT 
               
               
                   
                 AATCTCATTGGTTACCTTGGGCTATCGAAACTTAATTAAGCGATCTGCATCTCAATTAGTCAGCAAC 
               
               
                   
                 CATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCC 
               
               
                   
                 CATCGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGA 
               
               
                   
                 AGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGGAGGTAGCCAACATGATTGAACAAG 
               
               
                   
                 ATGGATTGCACGCAGGTTCTCCCGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACA 
               
               
                   
                 GACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTC 
               
               
                   
                 AAGACCGACCTGTCCGGTGCCCTGAATGAACTCCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCA 
               
               
                   
                 CGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATT 
               
               
                   
                 GGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATG 
               
               
                   
                 GCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAAC 
               
               
                   
                 ATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGA 
               
               
                   
                 GCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGGATGCCCGACGGCGAGGAT 
               
               
                   
                 CTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGAT 
               
               
                   
                 TCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATAT 
               
               
                   
                 TGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGAT 
               
               
                   
                 TCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTAGTATGTAAGCCCTGTGCCTTCTA 
               
               
                   
                 GTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCAC 
               
               
                   
                 TGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGG 
               
               
                   
                 GGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGG 
               
               
                   
                 TGGGCTCTATGGTTAATTAACCAGTCAAGTCAGCTACTTGGCGAGATCGACTTGTCTGGGTTTCGAC 
               
               
                   
                 TACGCTCAGAATTGCGTCAGTCAAGTTCGATCTGGTCCTTGCTATTGCACCCGTTCTCCGATTACGA 
               
               
                   
                 GTTTCATTTAAATCATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT 
               
               
                   
                 GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG 
               
               
                   
                 CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG 
               
               
                   
                 TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCA 
               
               
                   
                 TAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAA 
               
               
                   
                 CCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC 
               
               
                   
                 ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGC 
               
               
                   
                 TACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCT 
               
               
                   
                 CTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG 
               
               
                   
                 GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCC 
               
               
                   
                 TTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATG 
               
               
                   
                 AGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA 
               
               
                   
                 GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGAT 
               
               
                   
                 CTGTCTATTTCGTTCATCCATAGTTGCATTTAAATTTCCGAACTCTCCAAGGCCCTCGTCGGAAAAT 
               
               
                   
                 CTTCAAACCTTTCGTCCGATCCATCTTGCAGGCTACCTCTCGAACGAACTATCGCAAGTCTCTTGGC 
               
               
                   
                 CGGCCTTGCGCCTTGGCTATTGCTTGGCAGCGCCTATCGCCAGGTATTACTCCAATCCCGAATATCC 
               
               
                   
                 GAGATCGGGATCACCCGAGAGAAGTTCAACCTACATCCTCAATCCCGATCTATCCGAGATCCGAGGA 
               
               
                   
                 ATATCGAAATCGGGGCGCGCCTGGTGT (SEQ ID NO: 110) 
               
               
                   
               
            
           
         
       
     
     Example 22. Effects of Introducing a Nuclear Export Signal on Retrotransposition by Human LINE1 Constructs 
     In this example, addition of a nuclear export sequence is tested for improving translation of the cargo sequence. Addition of an NES at the C terminal or N terminal of the GFP is tested for increase in expression. Addition of multiple NESs at the C terminal or N terminal of the GFP is tested for increase in expression. Larger cargo (larger than 5 kB coding sequence, or larger than 7.5 kB coding sequence), will be tested to see if NES has effect on cargo of certain sizes. Additional cargo may be tested that specifically require trans-golgi or ER localization, e.g., cargo having a transmembrane domain. 
     Example 23. Screen for Efficient LINE1 System for Retrotransposition of Various Human Cells 
     In this example, a phylogenetic screen is undertaken across a large number of organisms using bioinformatics followed by laboratory testing, for selecting one or more hyperactive retrotransposon element that can efficiently move within human genome. Over 10 million LINE-1 sequences from 503 different genomes were identified (Ivancevic et al., 2016), including ORF1 and ORF2 proteins with novel domain variations. Among these, the ‘hyperactive’LINE-1 species were shown to display retrotransposition activity superior to that of human, rat and mouse. These elements will be cloned into LINE-1-GFPai plasmid and screened in HEK293T cells. Test mRNA constructs comprising a sequence encoding GFP along with a promoter and a poly A sequence, inserted in reverse orientation relative to the ORF1/2 genes in the retrotransposon complex as described elsewhere in the specification are used for electroporating the cells. Efficiency of integration is determined by flow cytometry and PCR. As above, GFP is measured in negative control and positive control to set gates for flow cytometry. As above, an exemplary negative control used will be set at &lt;0.1% GFP+. As above, an exemplary positive control used will be set at &gt;90% GFP+. Integration will be tested using PCR. An exemplary measurement indicative of successful integration is measurement of GFP expression, as determined by flow cytometry, in &gt;10% of cells by day 10 post retrotransposition. 
     Example 24. Improvement of Cargo Gene Expression 
     To improve the cargo gene expression, various cargo gene promoters and poly(A) signals will be tested. To improve the cargo gene expression, various insertions of a short constitutively spliced introns will also be tested. To improve the cargo gene expression, codon optimized sequences, such as those generated using various alternative codon algorithms, will also be tested. As above, GFP is measured in negative control and positive control to set gates for flow cytometry. As above, an exemplary negative control used will be set at &lt;0.1% GFP+. As above, an exemplary positive control used will be set at &gt;90% GFP+. An exemplary measurement indicative of successful cargo gene expression is measurement of increased GFP expression, as determined by flow cytometry, in &gt;10% of cells by day 10 post retrotransposition. 
     Example 25. Adapting Efficiency-Optimized Retrotransposon System for In Vivo Cargo Gene Delivery 
     The retrotransposon mRNA will be engineered for in vivo delivery using liver-targeting LNPs to detect an intracellular or secreted protein, in mice. Cell-type specific miRNA targets in the 3′ UTR of the cargo gene mRNA will be applied to target it for degradation in undesirable cell types. LNPs will be tested for efficient delivery in vivo. Mice will be administered the LNP encapsulated mRNA constructs that comprise a sequence encoding GFP along with a promoter and a poly A sequence flanking the GFP encoding sequence. The GFP gene is inserted in reverse orientation relative to the ORF1/2 genes in the retrotransposon complex as described elsewhere in the specification. Protein expression will be examined by imaging and test mice will be sacrificed and tissues harvested for histological analysis and PCR at determined time points. 
     Cell-specific targeting is achieved following systemic administration of the LNP encapsulated mRNA wherein the LNPs comprise surface modification to display antibodies or ligands that recognize cognate counterparts in the target cell. 
     Cell specific targeting is tested following designing specific constructs, wherein the UTRs are modified to encode one or more cell-type specific miRNA. When the recombinant mRNA is taken up by a cell that is not the intended target cell, miRNA present in the cell will bind to and destroy the mRNA. Hence the mRNA can express in the intended cell type. 
     An exemplary measurement indicative of successful in vivo mRNA delivery to liver measurement of GFP expression, as determined by flow cytometry or histology in hepatocytes. 
     Example 26. Targeting Retrotransposition to Specific Genomic Locations 
     In this example, retrotransposition design modifications are undertaken for increasing specificity of targeting at specific genomic locations. Retrotransposon fusion constructs are generated in which a targeting moiety is incorporated to ensure increase in targeting specificity of the ORF2p. One method is to retarget LINE1 elements with cargo to specifically integrate into one of the genomic safe harbor (GHS) sites with a high and sustainable transcriptional activity. AAVS1, CCR5 and ROSA26 are some of the GHS sites. As described above, specific LINE1 elements are selected after suitable screen for identifying an efficient LINE1 element that readily transposes in human. The selected LINE1 can be one that integrates into one of the GHS loci. 
     Another method is that the retrotransposon ORF2 is engineered bearing fusion of ORF2 with Cas9 and its mutants. Other candidates elements having a heterologous DNA binding domain with or without endonuclease activity are Cpf1, zinc finger element, TAL effector, Cas6-8 ‘cascade’, restriction endonuclease) which will either replace or complement the endonuclease domain of ORF2p. 
     The addition of homology arms of different length around the mRNA cargo will also be tested. 
     An exemplary construct is designed, comprising sequences encoding: ORF1; RT and dCas9; the cargo gene in antisense orientation, and a guide RNA to target site for priming RT activity. In one exemplary construct use of two guide RNA is tested, each fused to a dCas9 nickase, to determine if introducing nicks upstream and downstream of targeted locations improves site-specific integration and orientation of gene cargo. 
     Exemplary constructs are designed and tested in which the ORF2p lacks an EN (deleted), and is fused with a Cas 9 or its mutants, a Cas 12a (Cpf1), a Cas6-8 ‘cascade’, a Meganuclease, a Zinc-finger nuclease, a TALEN, or a restriction endonuclease, which directs the specificity and introduces the nicks on the genomic DNA. 
     Exemplary constructs are designed and tested in which the ORF2p comprises a mutant EN that has reduced or altered activity, and is fused with a Cas 9 or its mutants, a Cas 12a (Cpf1), a Cas6-8 ‘cascade’, a Meganuclease, a Zinc-finger nuclease, a TALEN, or a restriction endonuclease, which directs the specificity and introduces the nicks on the genomic DNA. For example, constructs are designed and tested in which retrotransposon plasmids will be engineered that encode a fusion of ORF2 protein with Cas9 and Cas9 mutants. mRNA containing ORF1, and mRNA encoding RT and dCas9 and the cargo gene in antisense orientation, and pegRNA will be delivered to target sites for priming RT activity. The use of two sgRNA, each fused to a dCas9 nickase, may be evaluated to determine if introducing nicks upstream and downstream of targeted locations improves site-specific integration and orientation of gene cargo. If modified LINE-1 RT fusion protein can bind its own mRNA and transport to nucleus, pegRNA could target genomic region and prime RT activity (via dCas9 nickase activity) so the LINE-1 RT can reverse transcribe mRNA gene cargo for integration into genomic DNA. 
     Efficiency of integration will be determined by flow cytometry. above, GFP is measured in negative control and positive control to set gates for flow cytometry. As above, an exemplary negative control used will be set at &lt;0.1% GFP+. As above, an exemplary positive control used will be set at &gt;90% GFP+. Integration will be confirmed by PCR. The sites of genomic integration are analyzed using NGS. Any construct with preferential integration in the specific genome location is subjected to several cycles of directed evolution to improve its efficiency and/or integration precision. An exemplary measurement indicative of successful gene integration and expression is 90% of cargo gene integrations detected in a specific genome location and GFP expression, as determined by flow cytometry, in &gt;2% of cells by day 10 post retrotransposition.