Patent Publication Number: US-8119130-B2

Title: Targeted binding agents directed to KDR and uses thereof—035

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application is a National Stage Application of International Application No. PCT/GB08/50615, filed Jul. 23, 2008, said International Application No. PCT/GB08/50615 claims the benefit of U.S. Appl. Ser. No. 60/951,805 filed Jul. 25, 2007, all of which are hereby incorporated by reference in their entirety for all purposes. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The invention relates to targeted binding agents against KDR and uses of such agents. More specifically, the invention relates to fully human monoclonal antibodies directed to KDR. The described targeted binding agents are useful in the treatment of diseases associated with the activity and/or overproduction of KDR and as diagnostics. 
     2. Description of the Related Art 
     The vascular endothelial growth factor-A (VEGF-A) plays a critical role in inducing vascular growth and remodeling during development, and in a number of pathological conditions including the angiogenesis required to support solid tumour growth. VEGF-A signaling is predominantly mediated through activation of VEGF receptor 2 (VEGFR2; KDR/flk-1), which can stimulate endothelial cell proliferation, migration, vascular permeability, and neovascular survival (reviewed in Olsson et al., Nat. Rev. Mol. Cell. Biol. 2006; 7:359-71.). The broader VEGFR family of tyrosine kinase receptors consists of three members: VEGFR1, VEGFR2 and VEGFR3, also known as Flt-1, KDR/Flk-1 and Flt4, respectively. Known VEGFR ligands exhibit differential, well-defined selectivity for each VEGFR. For example, VEGF-A binds both VEGFR1 and VEGFR2, VEGF-B and P1GF primarily bind VEGFR1, and VEGF-C and D are specific activators of VEGFR3. There are also a number of non-physiological VEGFs that also activate the VEGFR, for example VEGF-E specifically activates VEGFR2 while VEGF-F will activate VEGFR1 and VEGFR2. 
     Dimerization of VEGFR drives a complex series of signaling events leading to activation of many common growth factor signaling pathways (Olsson et al Nat. Rev. Mol. Cell Biol. 2006; 7:359-71). At the molecular level, there is a complex relationship between ligand expression, receptor dimerization and activation, and the downstream consequences in different cell types. VEGFR signaling is of primary importance to endothelial cells, although the receptors have been implicated in regulating the function of other cells. For example Flt-1 plays a role in mediating monocyte transmigration and, when expressed, can promote tumour cell migration. 
     All three receptors form both homo- and heterodimers (with the exception of VEGFR1/3 heterodimers), allowing signals from the various VEGF ligands to be integrated. KDR appears to be the receptor that is central to many of these signaling events, as it is the common VEGFR expressed on blood and lymphatic vessels. KDR can also offset low VEGFR-1 signaling activity. VEGFR1 itself has low intrinsic kinase activity, and deletion of the VEGFR1 kinase domain does not affect normal development. Experiments have shown that VEGFR1 can synergize with KDR and facilitate full activation of KDR signaling (Carmeliet et al, Nat Med 2001, 7, 575; Auterio et al, 2003 Nat Med, 9, 936). It is possible that this is achieved through heterodimerisation, which is prevented by an inhibitor of KDR dimerization. VEGFR3 has also been shown to form a heterodimer with KDR (Alam et al, BBRC, 2004, 324, 909). However, the mechanisms by which the various homo- and heterodimers of VEGFR1, KDR and VEGFR3 interact to drive physiological effects is unclear. Inhibition of VEGFR/VEGF signaling may effect different disease states (reviewed in Baka et al Expert Opin Ther Targets 2006, 10, 867). 
     VEGFRs consist of seven immunoglobin-like extracellular domains. Ligands, (e.g. VEGFA and P1GF) that specifically bind VEGFRs have immunoglobin-like domains 2 and 3, with domain 2 making the primary contact and domain 3 determining the specificity of binding (Christinger et al JBC, 2004, 279, 10382; Fuh et al JBC 1998, 273, 11197). In contrast, domains 4-6 are involved in dimerization of the receptor complexes. Ligand binding that stabilizes the receptor complexes can prolong dimerization, allowing productive signaling to proceed. As receptor activation is a function of both binding and dimerization, receptor activation can be inhibited by inhibiting ligand-receptor binding or by blocking dimerization. Antibodies that block binding of ligand to the receptors have been described previously, for example, IMC1121b and its murine equivalent, DC101, both of which block binding of VEGF-A to KDR, and thereby block VEGF-A signaling through KDR, and are known to deliver an anti-tumour effect (Prewett et al Cancer Res 1999, 59, 5209; Lu et al, JBC 2003, 278, 43496). It has been reported both pre-clinically and clinically that as a direct consequence of inhibiting KDR signaling, there is a rebound increase in circulating VEGF-A levels. Antibodies such as IMC1121b which block VEGF-A binding to KDR are expected to be less efficacious under conditions of increasing VEGF-A concentrations, where competition for binding to KDR may be won over by VEGF-A rather than the antibody. 
     Thus there is a need to identify new means of inhibiting KDR signaling. 
     SUMMARY OF THE INVENTION 
     The present invention relates to targeted binding agents that specifically bind to KDR and inhibit the biological activity of KDR. Embodiments of the invention relate to targeted binding agents that specifically bind to KDR and inhibit receptor dimerisation. Embodiments of the invention also relate to targeted binding agents that specifically bind to KDR and inhibit binding of VEGF to KDR. Embodiments of the invention relate to fully human isolated targeted binding agents that specifically bind to KDR and inhibit binding of VEGF to KDR Inhibition of KDR signaling by inhibition of receptor dimerisation is expected to have advantages over inhibition of VEGF-A binding to KDR. Targeted binding agents which inhibit receptor dimerisation, such as those described here, are anticipated to be able to maximally inhibit the KDR signaling axis by blocking KDR:KDR homodimer and KDR:Flt-1 heterodimer formation and hence block VEGF-A, VEGF-B and P1GF signaling through both KDR and Flt-1. In addition, increasing VEGF-A levels should have no direct impact on the efficacy of agents that inhibit receptor dimerization. 
     Embodiments of the invention relate to targeted binding agents that specifically bind to KDR and inhibit receptor dimerisation. In one embodiment the targeted binding agent that inhibits receptor dimerisation and binding of VEGF to KDR. In one embodiment of the invention the targeted binding agent specifically binds to KDR and inhibits KDR homodimer formation. In one embodiment of the invention the targeted binding agent specifically binds to KDR and inhibits KDR heterodimer formation. In one embodiment the targeted binding agent inhibits at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% of KDR receptor dimerisation that would occur in the absence of the targeted binding agent. 
     In one embodiment of the invention the targeted binding agent specifically binds to KDR and inhibits binding of VEGF. In one embodiment of the invention the targeted binding agent specifically binds to KDR and inhibits binding of VEGF-A. In one embodiment of the invention the targeted binding agent specifically binds to KDR and inhibits binding of PLGF. In one embodiment of the invention the targeted binding agent specifically binds to KDR and inhibits binding of VEGF-C, VEGF-D and/or VEGF-E. 
     In one embodiment the targeted binding agent inhibits at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% of VEGF or VEGF-C binding to KDR that would occur in the absence of the targeted binding agent. 
     In one embodiment of the invention the targeted binding agent specifically binds to KDR and inhibits VEGF-mediated prostaglandin release. In one embodiment the targeted binding agent inhibits at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% of VEGF-mediated prostaglandin release that would occur in the absence of the targeted binding agent. 
     In some embodiments of the invention, the targeted binding agent at 133 nM inhibits greater than 50% of VEGF-165-mediated tyrosine phosphorylation induced by 2 nM VEGF-165 in human umbilical vein endothelial cells (HUVECs). In some embodiments of the invention, the targeted binding agent at 133 nM inhibits greater than 50% of VEGF-165-mediated tyrosine phosphorylation induced by 2 nM VEGF-165 in human umbilical vein endothelial cells (HUVECs) as measured in an assay wherein the HUVECs are firstly incubated in FCS supplemented media, which is then replaced with supplement-free media overnight, the targeted binding agent is then added and after a pre-incubation period with the targeted binding agent, the cells are stimulated by addition of the VEGF-165. In some embodiments of the invention, the targeted binding agent inhibits greater than 50% of VEGF-165-mediated tyrosine phosphorylation in human umbilical vein endothelial cells (HUVECs) as measured in an assay as described in Example 11 herein. 
     In some embodiments of the invention, the targeted binding agent at a 1/20 dilution of hybridoma supernatant inhibits greater than 40% of VEGF-E mediated tyrosine phosphorylation induced by 2 nM VEGF-E in HUVECs. In some embodiments of the invention, the targeted binding agent at a 1/20 dilution of hybridoma supernatant inhibits greater than 40% of VEGF-E mediated tyrosine phosphorylation induced by 2 nM VEGF-E in HUVECs as measured in an assay wherein the HUVECs are firstly incubated in FCS plus growth supplements media, which is then replaced by supplement-free media overnight, followed by addition of the targeted binding agent and finally by replacement with the VEGF-E. In some embodiments of the invention, the targeted binding agent inhibits greater than 40% of VEGF-E mediated tyrosine phosphorylation in HUVECs as measured in an assay as described in Example 8 herein. 
     In some embodiments, the targeted binding agent at a 1/20 dilution of hybridoma supernatant inhibits greater than 55% of VEGF-E mediated cell survival as induced by 1 nM VEGF-E in HUVECs. In some embodiments, the targeted binding agent at a 1/20 dilution of hybridoma supernatant inhibits greater than 55% of VEGF-E mediated cell survival as induced by 1 nM VEGF-E in HUVECs as measured in an assay wherein the HUVECs are firstly incubated in FCS plus growth supplements media, followed by addition of the targeted binding agent and after a pre-incubation period, addition of VEGF-E. In some embodiments, the targeted binding agent inhibits greater than 55% of VEGF-E mediated cell survival in HUVECs as measured in an assay as described in Example 8 herein. 
     In some embodiments of the invention, the targeted binding agent at 20 μg/mL, 5 μg/mL, 1.25 μg/mL or 0.3125 μg/mL inhibits greater than 50% of endothelial cell tube formation in comparison with a control antibody. In some embodiments of the invention, the targeted binding agent at 20 μg/mL, 5 μg/mL, 1.25 μg/mL or 0.3125 μg/mL inhibits greater than 50% of endothelial cell tube formation as measured in an assay wherein the targeted binding agent is introduced to co-cultures of HUVECs and human diploid fibroblasts maintained in either TCS Optimised Medium or MCDB131 medium supplemented with 2% foetal calf serum, 1% glutamine and 1% penicillin/streptomycin. In some embodiments of the invention, the targeted binding agent at 20 μg/mL, 5 μg/mL, 1.25 μg/mL or 0.3125 μg/mL inhibits greater than 50% of endothelial cell tube formation as measured in an assay as described in Example 23. In some embodiments of the invention, the targeted binding agent at 20 μg/mL, 5 μg/mL, 1.25 μg/mL or 0.3125 μg/mL inhibits greater than 60%, 70%, 80% or 90% of endothelial cell tube formation. 
     In some embodiments of the invention, the targeted binding agent dosed at 10 mg/kg or 1 mg/kg twice weekly inhibits greater than 50% of angiogenesis in vivo. In some embodiments of the invention, the targeted binding agent dosed at 10 mg/kg or 1 mg/kg twice weekly inhibits greater than 50% of angiogenesis in vivo in a spheroid-based in vivo angiogenesis assay. In some embodiments of the invention, the targeted binding agent inhibits greater than 50% of angiogenesis in vivo as measured in an assay wherein HUVEC spheroids are mixed in a Matrigel/fibrin solution with single HUVECs to reach a final number of 100,000 ECs as spheroids and 200,000 single ECs per injected plug; VEGF-A and FGF added at a final concentration of 1000 ng/ml and the 500 μl of cell/matrix suspension injected into the study animal, with treatment with the targeted binding agent commenced the following day and ceased at day 21. In some embodiments of the invention, the targeted binding agent inhibits greater than 50% of angiogenesis in vivo as measured in an assay as described in Example 24. In some embodiments of the invention, the targeted binding agent inhibits greater than 60%, 70%, 80% or 90% of angiogenesis in vivo. 
     In some embodiments of the invention, the targeted binding agent binds KDR with a binding affinity (Kd) of less than 5 nanomolar (nM). In other embodiments, the targeted binding agent binds with a Kd of less than 4 nM, 3 nM, 2 nM or 1 nM. In some embodiments of the invention, the targeted binding agent binds KDR with a Kd of less than 950 picomolar (pM). In some embodiments of the invention, the targeted binding agent binds KDR with a Kd of less than 900 pM. In other embodiments, the targeted binding agent binds with a Kd of less than 800 pM, 700 pM or 600 pM. In some embodiments of the invention, the targeted binding agent binds KDR with a Kd of less than 500 pM. In other embodiments, the targeted binding agent binds with a Kd of less than 400 pM. In still other embodiments, the targeted binding agent binds with a Kd of less than 300 pM. In some other embodiments, the targeted binding agent binds with a Kd of less than 200 pM. The Kd may be assessed using a method described herein or known to one of skill in the art (e.g., a BIAcore assay, ELISA) (Biacore International AB, Uppsala, Sweden). 
     The binding properties of the targeted binding agent or antibody of the invention may also be measured by reference to the dissociation or association rates (k off  and k on  respectively). 
     In one embodiment of the invention, a targeted binding agent or an antibody may have an k on  rate (antibody (Ab)+antigen (Ag) k     on   →Ab−Ag) of at least 10 4 M −1 s −1 , at least 5×10 4 M −1 s −1 , at least 10 5 M −1 s −1 , at least 2×10 5 M −1 s −1 , at least 5×10 5 M −1 s −1 , at least 10 6 M −1 s −1 , at least 5×10 6 M −1 s −1 , at least 10 7 M −1 s −1 , at least 5×10 7 M −1 s −1 , or at least 10 8 M −1 s −1 . 
     In another embodiment of the invention, targeted binding agent or an antibody may have a k off  rate ((Ab−Ag) k     off   →antibody (Ab)+antigen (Ag)) of less than 5×10 −1 s −1 , less than 10 −1 s −1 , less than 5×10 −2 s −1 , less than 10 −2 s −1 , less than 5×10 −3 s −1 , less than 10 −3 s −1 , less than 5×10 −4 s −1 , less than 10 −4 s −1 , less than 5×10 −5 s −1 , less than 10 −5 s −1 , less than 5×10 −6 s −1 , less than 10 −6 s −1 , less than 5×10 −7 s −1 , less than 10 −7 s −1 , less than 5×10 −8 s −1 , less than 10 −8 s −1 , less than 5×10 −9 s −1 , less than 10 −9 s −1 , or less than 10 −10 s −1 . 
     In some embodiments of the invention, the targeted binding agent inhibits tumour growth and/or metastasis in a mammal. In other embodiments, the targeted binding agent ameliorates symptoms associated with inflammatory disorders in a mammal In one embodiment, the targeted binding agent ameliorates symptoms associated with inflammatory disorders selected from rheumatoid arthritis or psoriasis in a mammal. Symptoms that may be ameliorated include, but are not limited to, angiogenesis and synovitis. In still other embodiments, the targeted binding agent ameliorates symptoms associated with cardiovascular disease in a mammal In still other embodiments, the targeted binding agent ameliorates symptoms associated with a cardiovascular disease such as atherosclerosis in a mammal. Symptoms that may be ameliorated include, but are not limited to, inflammation and angiogenesis. In some other embodiments, the targeted binding agent ameliorates symptoms associated with sepsis in a mammal. Symptoms that may be ameliorated include, but are not limited to, uncontrolled vascular permeability, vascular leakage and angiogenesis. In some other embodiments, the targeted binding agent ameliorates symptoms associated with ocular disease. In some other embodiments, the targeted binding agent ameliorates symptoms associated with an ocular disease, such as ischaemic retinopathy or age-related macular degeneration. Symptoms that may be ameliorated include, but are not limited to, uncontrolled vascular permeability and vascular leakage. 
     In some embodiments of the invention, the targeted binding agent is an antibody. In some embodiments of the invention, the targeted binding agent is a monoclonal antibody. In one embodiment of the invention, the targeted binding agent is a fully human monoclonal antibody. In another embodiment of the invention, the targeted binding agent is a fully human monoclonal antibody of the IgG1, IgG2, IgG3 or IgG4 isotype. In another embodiment of the invention, the targeted binding agent is a fully human monoclonal antibody of the IgG2 isotype. This isotype has reduced potential to elicit effector function in comparison with other isotypes, which may lead to reduced toxicity. In another embodiment of the invention, the targeted binding agent is a fully human monoclonal antibody of the IgG1 isotype. The IgG1 isotype has increased potential to elicit ADCC in comparison with other isotypes, which may lead to improved efficacy. The IgG1 isotype has improved stability in comparison with other isotypes, e.g. IgG4, which may lead to improved bioavailability, or improved ease of manufacture or a longer half-life. In one embodiment, the fully human monoclonal antibody of the IgG1 isotype is of the z, za or f allotype. 
     A further embodiment is a targeted binding agent or an antibody that specifically binds to KDR and comprises a sequence comprising one of the complementarity determining regions (CDR) sequences shown in Table 20. Embodiments of the invention include a targeted binding agent or antibody comprising a sequence comprising: any one of a CDR1, a CDR2 or a CDR3 sequence as shown in Table 20. A further embodiment is a targeted binding agent or an antibody that specifically binds to KDR and comprises a sequence comprising two of the CDR sequences shown in Table 20. In another embodiment the targeted binding agent or antibody comprises a sequence comprising a CDR1, a CDR2 and a CDR3 sequence as shown in Table 20. In another embodiment the targeted binding agent or antibody comprises a sequence comprising one of the CDR sequences shown in Table 21. Embodiments of the invention include a targeted binding agent or antibody comprising a sequence comprising: any one of a CDR1, a CDR2 or a CDR3 sequence as shown in Table 21. In another embodiment the targeted binding agent or antibody comprises a sequence comprising two of the CDR sequences shown in Table 21. In another embodiment the targeted binding agent or antibody comprises a sequence comprising a CDR1, a CDR2 and a CDR3 sequence as shown in Table 21. In another embodiment the targeted binding agent or antibody may comprise a sequence comprising a CDR1, a CDR2 and a CDR3 sequence as shown in Table 20 and a CDR1, a CDR2 and a CDR3 sequence as shown in Table 21. In some embodiments, the targeted binding agent is an antibody. In certain embodiments, the targeted binding agent is a fully human monoclonal antibody. In certain other embodiments, the targeted binding agent is a binding fragment of a fully human monoclonal antibody. 
     For the avoidance of doubt, the term “Table 20” as used herein includes Table 20a and Table 20b. 
     For the avoidance of doubt, the term “Table 21” as used herein includes Table 21a and Table 21b. 
     It is noted that those of ordinary skill in the art can readily accomplish CDR determinations. See for example, Kabat et al.,  Sequences of Proteins of Immunological Interest , Fifth Edition, NIH Publication 91-3242, Bethesda Md. (1991), vols. 1-3. Kabat provides multiple sequence alignments of immunoglobulin chains from numerous species antibody isotypes. The aligned sequences are numbered according to a single numbering system, the Kabat numbering system. The Kabat sequences have been updated since the 1991 publication and are available as an electronic sequence database (latest downloadable version 1997). Any immunoglobulin sequence can be numbered according to Kabat by performing an alignment with the Kabat reference sequence. Accordingly, the Kabat numbering system provides a uniform system for numbering immunoglobulin chains. 
     In one embodiment, the targeted binding agent or antibody comprises a sequence comprising any one of the heavy chain sequences shown in Table 20. In another embodiment, the targeted binding agent or antibody comprises a sequence comprising any one of the heavy chain sequences of antibodies 33D5, 29H3, 29F7, 33C3, 31E11, 21A1, 21H6, 24C9, 32G7, 24B3, 33B1, 29A11, 30H10, 32B2, 32C11, 30E3, 1G6, 30F6, 30D7, 21H9, 29A3, 33E1, 22B8, 27A3, 27D10, 30A1, 32F4 or 29D4. Light-chain promiscuity is well established in the art, thus, a targeted binding agent or antibody comprising a sequence comprising any one of the heavy chain sequences of antibodies 33D5, 29H3, 29F7, 33C3, 31E11, 21A1, 21H6, 24C9, 32G7, 24B3, 33B1, 29A11, 30H10, 32B2, 32C11, 30E3, 1G6, 30F6, 30D7, 21H9, 29A3, 33E1, 22B8, 27A3, 27D10, 30A1, 32F4 or 29D4 or another antibody as disclosed herein, may further comprise any one of the light chain sequences shown in Table 21 or of antibodies 33D5, 29H3, 29F7, 33C3, 31E11, 21A1, 21H6, 24C9, 32G7, 24B3, 33B1, 29A11, 30H10, 32B2, 32C11, 30E3, 1G6, 30F6, 30D7, 21H9, 29A3, 33E1, 22B8, 27A3, 27D10, 30A1, 32F4 or 29D4, or another antibody as disclosed herein. In some embodiments, the antibody is a fully human monoclonal antibody. 
     In one embodiment, the targeted binding agent or antibody comprises a sequence comprising any one of the light chain sequences shown in Table 21. In another embodiment, the targeted binding agent or antibody comprises a sequence comprising any one of the light chain sequences of antibodies 33D5, 29H3, 29F7, 33C3, 31E11, 21A1, 21H6, 24C9, 32G7, 24B3, 33B1, 29A11, 30H10, 32B2, 32C11, 30E3, 1G6, 30F6, 30D7, 21H9, 29A3, 33E1, 22B8, 27A3, 27D10, 30A1, 32F4 or 29D4. In some embodiments, the antibody is a fully human monoclonal antibody. 
     In some embodiments, the targeting binding agent is a monoclonal antibody selected from the group consisting of: 24B3, 27D10 and 33C3. In one embodiment, the targeted binding agent comprises one or more of fully human monoclonal antibodies 24B3, 27D10 or 33C3. In certain embodiments, the targeting binding agent is monoclonal antibody 24B3. In certain other embodiments, the targeting binding agent is monoclonal antibody 27D10. In still other embodiments, the targeting binding agent is monoclonal antibody 33C3. In additional embodiments, the targeted binding agent is derivable from any of the foregoing monoclonal antibodies. 
     In one embodiment a targeted binding agent or an antibody may comprise a sequence comprising a heavy chain CDR1, CDR2 and CDR3 selected from any one of the sequences shown in Table 20. In one embodiment a targeted binding agent or an antibody may comprise a sequence comprising a light chain CDR1, CDR2 and CDR3 selected from any one of the sequences shown in Table 21. In one embodiment a targeted binding agent or an antibody may comprise a sequence comprising a heavy chain CDR1, CDR2 and CDR3 selected from any one of the CDRs of antibodies 33D5, 29H3, 29F7, 33C3, 31E11, 21A1, 21H6, 24C9, 32G7, 24B3, 33B1, 29A11, 30H10, 32B2, 32C11, 30E3, 1G6, 30F6, 30D7, 21H9, 29A3, 33E1, 22B8, 27A3, 27D10, 30A1, 32F4 or 29D4. In one embodiment a targeted binding agent or an antibody may comprise a sequence comprising a light chain CDR1, CDR2 and CDR3 selected from any one of the CDRs of antibodies 33D5, 29H3, 29F7, 33C3, 31E11, 21A1, 21H6, 24C9, 32G7, 24B3, 33B1, 29A11, 30H10, 32B2, 32C11, 30E3, 1G6, 30F6, 30D7, 21H9, 29A3, 33E1, 22B8, 27A3, 27D10, 30A1, 32F4 or 29D4. 
     In another embodiment the targeted binding agent or antibody may comprise a sequence comprising any one of a CDR1, a CDR2 or a CDR3 of any one of the fully human monoclonal antibodies 24B3, 27D10 or 33C3, as shown in Table 20. In another embodiment the targeted binding agent or antibody may comprise a sequence comprising any one of a CDR1, a CDR2 or a CDR3 of any one of the fully human monoclonal antibodies 24B3, 27D10 or 33C3, as shown in Table 21. In one embodiment the targeted binding agent or antibody may comprise a sequence comprising a CDR1, a CDR2 and a CDR3 of fully human monoclonal antibody 24B3, 27D10 or 33C3, as shown in Table 20. In another embodiment the targeted binding agent or antibody may comprise a sequence comprising a CDR1, a CDR2 and a CDR3 of fully human monoclonal antibody 24B3, 27D10 or 33C3, as shown in Table 21. In another embodiment the targeted binding agent or antibody may comprise a sequence comprising a CDR1, a CDR2 and a CDR3 of fully human monoclonal antibody 24B3, 27D10 or 33C3, as shown in Table 20, and a CDR1, a CDR2 and a CDR3 sequence of fully human monoclonal antibody 24B3, 27D10 or 33C3, as shown in Table 21. In some embodiments, the antibody is a fully human monoclonal antibody. 
     In another embodiment the targeted binding agent or antibody comprises a sequence comprising the CDR1, CDR2 and CDR3 sequence of fully human monoclonal antibody 24B3 as shown in Table 20 and the CDR1, CDR2 and CDR3 sequence of fully human monoclonal antibody 24B3 as shown in Table 21. In another embodiment the targeted binding agent or antibody comprises a sequence comprising the CDR1, CDR2 and CDR3 sequence of fully human monoclonal antibody 27D10 as shown in Table 20 and the CDR1, CDR2 and CDR3 sequence of fully human monoclonal antibody 27D10 as shown in Table 21. In another embodiment the targeted binding agent or antibody comprises a sequence comprising the CDR1, CDR2 and CDR3 sequence of fully human monoclonal antibody 33C3 as shown in Table 20 and the CDR1, CDR2 and CDR3 sequence of fully human monoclonal antibody 33C3 as shown in Table 21. In some embodiments, the antibody is a fully human monoclonal antibody. 
     A further embodiment of the invention is a targeted binding agent or antibody comprising a sequence comprising the contiguous sequence spanning the framework regions and CDRs, specifically from FR1 through FR4 or CDR1 through CDR3, of any one of the sequences as shown in Table 20 or Table 21. In one embodiment the targeted binding agent or antibody comprises a sequence comprising the contiguous sequences spanning the framework regions and CDRs, specifically from FR1 through FR4 or CDR1 through CDR3, of any one of the sequences of monoclonal antibodies 24B3, 27D10 or 33C3, as shown in Table 20 or Table 21. In some embodiments, the antibody is a fully human monoclonal antibody. 
     One embodiment provides a targeted binding agent or antibody, or antigen-binding portion thereof, wherein the agent or antibody, or antigen-binding portion thereof, comprises a heavy chain polypeptide comprising the sequence of SEQ ID NO.:42. In one embodiment, the agent or antibody, or antigen-binding portion thereof, further comprises a light chain polypeptide comprising the sequence of SEQ ID NO.:44. In some embodiments, the antibody is a fully human monoclonal antibody. 
     In another embodiment the agent or antibody, or antigen-binding portion thereof, comprises a heavy chain polypeptide comprising the sequence of SEQ ID NO.:14. In one embodiment, the agent or antibody, or antigen-binding portion thereof, further comprises a light chain polypeptide comprising the sequence of SEQ ID NO.:16. In some embodiments, the antibody is a fully human monoclonal antibody. 
     In another embodiment the agent or antibody, or antigen-binding portion thereof, comprises a heavy chain polypeptide comprising the sequence of SEQ ID NO.:74. In another embodiment, the agent or antibody, or antigen-binding portion thereof, further comprises a light chain polypeptide comprising the sequence of SEQ ID NO.:76. In some embodiments, the antibody is a fully human monoclonal antibody. 
     In one embodiment the targeted binding agent or antibody comprises as many as twenty, sixteen, ten, nine or fewer, e.g. one, two, three, four or five, amino acid additions, substitutions, deletions, and/or insertions within the disclosed CDRs or heavy or light chain sequences. Such modifications may potentially be made at any residue within the CDRs. In some embodiments, the antibody is a fully human monoclonal antibody. 
     In one embodiment, the targeted binding agent or antibody comprises variants or derivatives of the CDRs disclosed herein, the contiguous sequences spanning the framework regions and CDRs (specifically from FR1 through FR4 or CDR1 through CDR3), the light or heavy chain sequences disclosed herein, or the antibodies disclosed herein. Variants include targeted binding agents or antibodies comprising sequences which have as many as twenty, sixteen, ten, nine or fewer, e.g. one, two, three, four, five or six amino acid additions, substitutions, deletions, and/or insertions in any of the CDR1, CDR2 or CDR3s as shown in Table 20 or Table 21, the contiguous sequences spanning the framework regions and CDRs (specifically from FR1 through FR4 or CDR1 through CDR3) as shown in Table 20 or Table 21, the light or heavy chain sequences disclosed herein, or with the monoclonal antibodies disclosed herein. Variants include targeted binding agents or antibodies comprising sequences which have at least about 60, 70, 80, 85, 90, 95, 98 or about 99% amino acid sequence identity with any of the CDR1, CDR2 or CDR3s as shown in Table 20 or Table 21, the contiguous sequences spanning the framework regions and CDRs (specifically from FR1 through FR4 or CDR1 through CDR3) as shown in Table 20 or Table 21, the light or heavy chain sequences disclosed herein, or with the monoclonal antibodies disclosed herein. The percent identity of two amino acid sequences can be determined by any method known to one skilled in the art, including, but not limited to, pairwise protein alignment. In one embodiment variants comprise changes in the CDR sequences or light or heavy chain polypeptides disclosed herein that are naturally occurring or are introduced by in vitro engineering of native sequences using recombinant DNA techniques or mutagenesis techniques. Naturally occurring variants include those which are generated in vivo in the corresponding germline nucleotide sequences during the generation of an antibody to a foreign antigen. In one embodiment the derivative may be a heteroantibody, that is an antibody in which two or more antibodies are linked together. Derivatives include antibodies which have been chemically modified. Examples include covalent attachment of one or more polymers, such as water-soluble polymers, N-linked, or O-linked carbohydrates, sugars, phosphates, and/or other such molecules. The derivatives are modified in a manner that is different from the naturally occurring or starting antibody, either in the type or location of the molecules attached. Derivatives further include deletion of one or more chemical groups which are naturally present on the antibody. 
     In one embodiment, the targeted binding agent is a bispecific antibody. A bispecific antibody is an antibody that has binding specificity for at least two different epitopes. Methods for making bispecific antibodies are known in the art. (See, for example, Millstein et al.,  Nature,  305:537-539 (1983); Traunecker et al.,  EMBO J.,  10:3655-3659 (1991); Suresh et al.,  Methods in Enzymology,  121:210 (1986); Kostelny et al.,  J. Immunol.,  148(5):1547-1553 (1992); Hollinger et al.,  Proc. Natl. Acad. Sci. USA,  90:6444-6448 (1993); Gruber et al.,  J. Immunol.,  152:5368 (1994); U.S. Pat. Nos. 4,474,893; 4,714,681; 4,925,648; 5,573,920; 5,601,81; 95,731,168; 4,676,980; and 4,676,980, WO 94/04690; WO 91/00360; WO 92/200373; WO 93/17715; WO 92/08802; and EP 03089.) 
     In some embodiments of the invention, the targeted binding agent or antibody comprises a sequence comprising SEQ ID NO.: 42. In certain embodiments, SEQ ID NO.:42 comprises any one of the combinations of germline and non-germline residues indicated by each row of Table 17. In some embodiments, SEQ ID NO:42 comprises any one, any two, any three, any four, any five, any six, any seven, any eight or all nine of the germline residues as indicated in Table 17. In certain embodiments, SEQ ID NO.:42 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 17a. In other embodiments, the targeted binding agent or antibody is derived from a germline sequence with VH3-21, D3-10 and JH4B domains, wherein one or more residues has been mutated to yield the corresponding germline residue at that position. 
     In some embodiments of the invention, the targeted binding agent or antibody comprises a sequence comprising SEQ ID NO.:44. In certain embodiments, SEQ ID NO.:44 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 16. In some embodiments, SEQ ID NO:44 comprises any one, any two or all three of the germline residues as indicated in Table 16. In certain embodiments, SEQ ID NO.:44 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 16a. In other embodiments, the targeted binding agent or antibody is derived from a germline sequence with A30 and JK1 domains, wherein one or more residues has been mutated to yield the corresponding germline residue at that position. 
     In some embodiments of the invention, in the targeted binding agent or antibody comprises a sequence comprising SEQ ID NO.:14. In certain embodiments, SEQ ID NO.:14 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 15. In some embodiments, SEQ ID NO:14 comprises any one, any two, any three, any four, any five or all six of the germline residues as indicated in Table 15. In certain embodiments, SEQ ID NO.:14 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 15a. In other embodiments, the targeted binding agent or antibody is derived from a germline sequence with VH4-39, D6-6 and JH4B domains, wherein one or more residues has been mutated to yield the corresponding germline residue at that position. 
     In some embodiments of the invention, the targeted binding agent or antibody comprises a sequence comprising SEQ ID NO.:16. In certain embodiments, the targeted binding agent or antibody is derived from a germline sequence with A27 and JK4 domains, wherein one or more residues has been mutated to yield the corresponding germline residue at that position. 
     In some embodiments of the invention, the targeted binding agent or antibody comprises a sequence comprising SEQ ID NO.:74. In certain embodiments, SEQ ID NO.:74 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 19. In some embodiments, SEQ ID NO:74 comprises any one, any two, any three, any four, any five, any six, any seven or all eight of the germline residues as indicated in Table 19. In certain embodiments, SEQ ID NO.:74 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 19a. In other embodiments, the targeted binding agent or antibody is derived from a germline sequence with VH3-11, D3-3 and JH5B domains, wherein one or more residues has been mutated to yield the corresponding germline residue at that position. 
     In some embodiments of the invention, the targeted binding agent or antibody comprises a sequence comprising SEQ ID NO.:76. In certain embodiments, SEQ ID NO.:76 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 18. In some embodiments, SEQ ID NO:76 comprises any one, any two, any three, any four, any five, any six, any seven, any eight or all nine of the germline residues as indicated in Table 18. In certain embodiments, SEQ ID NO.:76 comprises any one of the unique combinations of germline and non-germline residues indicated by each row of Table 18a. In other embodiments, the targeted binding agent or antibody is derived from a germline sequence with O2 and JK4 domains, wherein one or more residues has been mutated to yield the corresponding germline residue at that position. 
     A further embodiment of the invention is a targeted binding agent or antibody which competes for binding to KDR with the targeted binding agent or antibodies of the invention. In another embodiment of the invention there is an antibody which competes for binding to KDR with the targeted binding agent or antibodies of the invention. In another embodiment the targeted binding agent or antibody competes for binding to KDR with any one of fully human monoclonal antibodies 24B3, 27D10 or 33C3. “Competes” indicates that the targeted binding agent or antibody competes for binding to KDR with any one of fully human monoclonal antibodies 24B3, 27D10 and 33C3, i.e. competition is unidirectional. 
     Embodiments of the invention include a targeted binding agent or antibody which cross competes with any one of fully human monoclonal antibodies 24B3, 27D10 and 33C3 for binding to KDR. “Cross competes” indicates that the targeted binding agent or antibody competes for binding to KDR with any one of fully human monoclonal antibodies 24B3, 27D10 and 33C3, and vice versa, i.e. competition is bidirectional. 
     A further embodiment of the invention is a targeted binding agent or antibody which competes for binding to the dimerisation domain of KDR. In another embodiment of the invention there is a targeted binding agent or antibody which cross-competes with the targeted binding agent or antibodies of the invention for binding to the dimerisation domain of KDR. 
     A further embodiment of the invention is a targeted binding agent or antibody that binds to the same epitope on KDR as the targeted binding agent or antibodies of the invention. Embodiments of the invention also include a targeted binding agent or antibody that binds to the same epitope on KDR as any one of fully human monoclonal antibodies 24B3, 27D10 and 33C3. 
     Other embodiments of the invention include isolated nucleic acid molecules encoding any of the targeted binding agents or antibodies described herein, vectors having isolated nucleic acid molecules encoding the targeted binding agents or antibodies described herein or a host cell transformed with any of such nucleic acid molecules. Embodiments of the invention include a nucleic acid molecule encoding a fully human isolated targeted binding agent that specifically bind to KDR and inhibit binding of VEGF to KDR. The invention also encompasses polynucleotides that hybridize under stringent or lower stringency hybridization conditions, as defined herein, to polynucleotides that encode any of the targeted binding agents or antibodies described herein. Embodiments of the invention also include a vector comprising the nucleic acid molecule encoding the binding agent. Additional embodiments include a host cell comprising the vector of comprising the nucleic acid molecule. 
     As known in the art, antibodies can advantageously be, for example, polyclonal, oligoclonal, monoclonal, chimeric, humanised, and/or fully human antibodies. 
     It will be appreciated that embodiments of the invention are not limited to any particular form of an antibody or method of generation or production. In some embodiments of the invention, the targeted binding agent is a binding fragment of a fully human monoclonal antibody. For example, the targeted binding agent can be a full-length antibody (e.g., having an intact human Fc region) or an antibody binding fragment (e.g., a Fab, Fab′ or F(ab′) 2 , FV or dAb). In addition, the antibodies can be single-domain antibodies such as camelid or human single VH or VL domains that bind to KDR, such as a dAb fragment. 
     Embodiments of the invention described herein also provide cells for producing these antibodies. Examples of cells include hybridomas, or recombinantly created cells, such as Chinese hamster ovary (CHO) cells, variants of CHO cells (for example DG44) and NS0 cells that produce antibodies against KDR. Additional information about variants of CHO cells can be found in Andersen and Reilly (2004)  Current Opinion in Biotechnology  15, 456-462 which is incorporated herein in its entirety by reference. The antibody can be manufactured from a hybridoma that secretes the antibody, or from a recombinantly engineered cell that has been transformed or transfected with a gene or genes encoding the antibody. 
     In addition, one embodiment of the invention is a method of producing an antibody of the invention by culturing host cells under conditions wherein a nucleic acid molecule is expressed to produce the antibody followed by recovering the antibody. It should be realised that embodiments of the invention also include any nucleic acid molecule which encodes an antibody or fragment of an antibody of the invention including nucleic acid sequences optimised for increasing yields of antibodies or fragments thereof when transfected into host cells for antibody production. 
     A further embodiment herein includes a method of producing antibodies that specifically bind to KDR and inhibit the biological activity of KDR, by immunising a mammal with cells expressing human KDR, isolated cell membranes containing human KDR, purified human KDR, or a fragment thereof, and/or one or more orthologous sequences or fragments thereof. 
     In other embodiments the invention provides compositions, including a targeted binding agent or antibody of the invention or binding fragment thereof, and a pharmaceutically acceptable carrier or diluent. 
     Still further embodiments of the invention include methods of effectively treating an animal suffering from a proliferative, angiogenic, cell adhesion or invasion-related disease by administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. In certain embodiments the method further comprises selecting an animal in need of treatment for a proliferative, angiogenic, cell adhesion or invasion-related disease, and administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. 
     Still further embodiments of the invention include methods of effectively treating an animal suffering from a neoplastic disease by administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. In certain embodiments the method further comprises selecting an animal in need of treatment for a neoplastic disease, and administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. 
     Still further embodiments of the invention include methods of effectively treating an animal suffering from a non-neoplastic disease by administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. In certain embodiments the method further comprises selecting an animal in need of treatment for a non-neoplastic disease, and administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. 
     Still further embodiments of the invention include methods of effectively treating an animal suffering from a malignant tumour by administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. In certain embodiments the method further comprises selecting an animal in need of treatment for a malignant tumour, and administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. 
     Still further embodiments of the invention include methods of effectively treating an animal suffering from a disease or condition associated with KDR expression by administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. In certain embodiments the method further comprises selecting an animal in need of treatment for a disease or condition associated with KDR expression, and administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. 
     Still further embodiments of the invention include methods of effectively treating an animal suffering from KDR induced disease-related VEGF activation by administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. In certain embodiments the method further comprises selecting an animal in need of treatment for KDR induced disease-related VEGF activation, and administering to the animal a therapeutically effective dose of a targeted binding agent that specifically binds to KDR. 
     A malignant tumour may be selected from the group consisting of: melanoma, small cell lung cancer, non-small cell lung cancer, glioma, hepatocellular (liver) carcinoma, thyroid tumour, gastric (stomach) cancer, prostate cancer, breast cancer, ovarian cancer, bladder cancer, lung cancer, glioblastoma, endometrial cancer, kidney cancer, colon cancer, pancreatic cancer, esophageal carcinoma, head and neck cancers, mesothelioma, sarcomas, biliary (cholangiocarcinoma), small bowel adenocarcinoma, pediatric malignancies and epidermoid carcinoma. 
     Treatable proliferative, angiogenic, cell adhesion or invasion-related diseases include neoplastic diseases, such as, melanoma, small cell lung cancer, non-small cell lung cancer, glioma, hepatocellular (liver) carcinoma, thyroid tumour, gastric (stomach) cancer, gallbladder cancer, prostate cancer, breast cancer, ovarian cancer, bladder cancer, lung cancer, glioblastoma, endometrial cancer, kidney cancer, colon cancer, pancreatic cancer, esophageal carcinoma, head and neck cancers, mesothelioma, sarcomas, biliary (cholangiocarcinoma), small bowel adenocarcinoma, pediatric malignancies, epidermoid carcinoma and leukaemia, including chronic myelogenous leukaemia. 
     In one embodiment, the neoplastic disease is melanoma, colon cancer or chronic myelogenous leukaemia. 
     Non-neoplastic diseases include inflammatory disorders such as rheumatoid arthritis or psoriasis, cardiovascular disease such as atherosclerosis, sepsis, ocular disease such as ischaemic retinopathy or age-related macular degeneration. 
     In one embodiment the present invention is suitable for use in inhibiting KDR, in patients with a tumour which is dependent alone, or in part, on KDR. 
     Still further embodiments of the invention include use of a targeted binding agent or antibody of the invention in the preparation of a medicament for the treatment of an animal suffering from a proliferative, angiogenic, cell adhesion or invasion-related disease. In certain embodiments the use further comprises selecting an animal in need of treatment for a proliferative, angiogenic, cell adhesion or invasion-related disease. 
     Still further embodiments of the invention include use of a targeted binding agent or antibody of the invention in the preparation of a medicament for the treatment of an animal suffering from a neoplastic disease. In certain embodiments the use further comprises selecting an animal in need of treatment for a neoplastic disease. 
     Still further embodiments of the invention include use of a targeted binding agent or antibody of the invention in the preparation of a medicament for the treatment of an animal suffering from a non-neoplastic disease. In certain embodiments the use further comprises selecting an animal in need of treatment for a non-neoplastic disease. 
     Still further embodiments of the invention include use of a targeted binding agent or antibody of the invention in the preparation of a medicament for the treatment of an animal suffering from a malignant tumour. In certain embodiments the use further comprises selecting an animal in need of treatment for a malignant tumour. 
     Still further embodiments of the invention include use of a targeted binding agent or antibody of the invention in the preparation of a medicament for the treatment of an animal suffering from a disease or condition associated with KDR expression. In certain embodiments the use further comprises selecting an animal in need of treatment for a disease or condition associated with KDR expression. 
     Still further embodiments of the invention include use of a targeted binding agent or antibody of the invention in the preparation of a medicament for the treatment of an animal suffering from KDR induced disease-related VEGF activation. In certain embodiments the use further comprises selecting an animal in need of treatment for KDR induced disease-related VEGF activation. 
     Still further embodiments of the invention include a targeted binding agent or antibody of the invention for use as a medicament for the treatment of an animal suffering from a proliferative, angiogenic, cell adhesion or invasion-related disease. 
     Still further embodiments of the invention include a targeted binding agent or antibody of the invention for use as a medicament for the treatment of an animal suffering from a neoplastic disease. 
     Still further embodiments of the invention include a targeted binding agent or antibody of the invention for use as a medicament for the treatment of an animal suffering from a non-neoplastic disease. 
     Still further embodiments of the invention include a targeted binding agent or antibody of the invention for use as a medicament for the treatment of an animal suffering from a malignant tumour. 
     Still further embodiments of the invention include a targeted binding agent or antibody of the invention for use as a medicament for the treatment of an animal suffering from a disease or condition associated with KDR expression. 
     Still further embodiments of the invention include a targeted binding agent or antibody of the invention for use as a medicament for the treatment of an animal suffering from KDR induced disease-related VEGF activation. 
     In one embodiment treatment of a
         a proliferative, angiogenic, cell adhesion or invasion-related disease;   a neoplastic disease;   a non-neoplastic disease;   a malignant tumour;   a disease or condition associated with KDR expression; or   KDR induced disease-related VEGF activation,       

     comprises managing, ameliorating, preventing, any of the aforementioned diseases or conditions. 
     In one embodiment treatment of a neoplastic disease comprises inhibition of tumour growth, tumour growth delay, regression of tumour, shrinkage of tumour, increased time to regrowth of tumour on cessation of treatment, increased time to tumour recurrence, slowing of disease progression. 
     In some embodiments of the invention, the animal to be treated is a human. 
     In some embodiments of the invention, the targeted binding agent is a fully human monoclonal antibody. 
     In some embodiments of the invention, the targeted binding agent is selected from the group consisting of fully human monoclonal antibodies 24B3, 27D10 and 33C3. 
     Embodiments of the invention include a conjugate comprising the targeted binding agent as described herein, and a therapeutic agent. In some embodiments of the invention, the therapeutic agent is a toxin. In other embodiments, the therapeutic agent is a radioisotope. In still other embodiments, the therapeutic agent is a pharmaceutical composition. 
     In another aspect, a method of selectively killing a cancerous cell in a patient is provided. The method comprises administering a fully human antibody conjugate to a patient. The fully human antibody conjugate comprises an antibody that can bind to KDR and an agent. The agent is either a toxin, a radioisotope, or another substance that will kill a cancer cell. The antibody conjugate thereby selectively kills the cancer cell. 
     In one aspect, a conjugated fully human antibody that specifically binds to KDR is provided. Attached to the antibody is an agent, and the binding of the antibody to a cell results in the delivery of the agent to the cell. In one embodiment, the above conjugated fully human antibody binds to an extracellular domain of KDR. In another embodiment, the antibody and conjugated toxin are internalised by a cell that expresses KDR. In another embodiment, the agent is a cytotoxic agent. In another embodiment, the agent is, for example saporin, or auristatin, pseudomonas exotoxin, gelonin, ricin, calicheamicin or maytansine-based immunoconjugates, and the like. In still another embodiment, the agent is a radioisotope. 
     The targeted binding agent or antibody of the invention can be administered alone, or can be administered in combination with additional antibodies or chemotherapeutic drugs or radiation therapy. For example, a monoclonal, oligoclonal or polyclonal mixture of KDR antibodies that block cell adhesion, invasion, angiogenesis or proliferation can be administered in combination with a drug shown to inhibit tumour cell proliferation. 
     Another embodiment of the invention includes a method of diagnosing diseases or conditions in which an antibody as disclosed herein is utilised to detect the level of KDR in a patient or patient sample. In one embodiment, the patient sample is blood or blood serum or urine. In further embodiments, methods for the identification of risk factors, diagnosis of disease, and staging of disease is presented which involves the identification of the expression and/or overexpression of KDR using anti-KDR antibodies. In some embodiments, the methods comprise administering to a patient a fully human antibody conjugate that selectively binds to KDR on a cell. The antibody conjugate comprises an antibody that specifically binds to KDR and a label. The methods further comprise observing the presence of the label in the patient. A relatively high amount of the label will indicate a relatively high risk of the disease and a relatively low amount of the label will indicate a relatively low risk of the disease. In one embodiment, the label is a green fluorescent protein. 
     The invention further provides methods for assaying the level of KDR in a patient sample, comprising contacting an antibody as disclosed herein with a biological sample from a patient, and detecting the level of binding between said antibody and KDR in said sample. In more specific embodiments, the biological sample is blood, plasma or serum. 
     Another embodiment of the invention includes a method for diagnosing a condition associated with the expression of KDR in a cell by contacting the serum or a cell with an antibody as disclosed herein, and thereafter detecting the presence of KDR. In one embodiment the condition can be a proliferative, angiogenic, cell adhesion or invasion-related disease including, but not limited to, a neoplastic disease. 
     In another embodiment, the invention includes an assay kit for detecting KDR in mammalian tissues, cells, or body fluids to screen for KDR-related diseases. The kit includes an antibody as disclosed herein and a means for indicating the reaction of the antibody with KDR, if present. In one embodiment the antibody is a monoclonal antibody. In one embodiment, the antibody that binds KDR is labelled. In another embodiment the antibody is an unlabelled primary antibody and the kit further includes a means for detecting the primary antibody. In one embodiment, the means for detecting includes a labelled second antibody that is an anti-immunoglobulin. The antibody may be labelled with a marker selected from the group consisting of a fluorochrome, an enzyme, a radionuclide and a radiopaque material. 
     In some embodiments, the targeted binding agents or antibodies as disclosed herein can be modified to enhance their capability of fixing complement and participating in complement-dependent cytotoxicity (CDC). In other embodiments, the targeted binding agents or antibodies can be modified to enhance their capability of activating effector cells and participating in antibody-dependent cytotoxicity (ADCC). In yet other embodiments, the targeted binding agents or antibodies as disclosed herein can be modified both to enhance their capability of activating effector cells and participating in antibody-dependent cytotoxicity (ADCC) and to enhance their capability of fixing complement and participating in complement-dependent cytotoxicity (CDC). 
     In some embodiments, the targeted binding agents or antibodies as disclosed herein can be modified to reduce their capability of fixing complement and participating in complement-dependent cytotoxicity (CDC). In other embodiments, the targeted binding agents or antibodies can be modified to reduce their capability of activating effector cells and participating in antibody-dependent cytotoxicity (ADCC). In yet other embodiments, the targeted binding agents or antibodies as disclosed herein can be modified both to reduce their capability of activating effector cells and participating in antibody-dependent cytotoxicity (ADCC) and to reduce their capability of fixing complement and participating in complement-dependent cytotoxicity (CDC). 
     In certain embodiments, the half-life of a targeted binding agent or antibody as disclosed herein and of compositions of the invention is at least about 4 to 7 days. In certain embodiments, the mean half-life of a targeted binding agent or antibody as disclosed herein and of compositions of the invention is at least about 2 to 5 days, 3 to 6 days, 4 to 7 days, 5 to 8 days, 6 to 9 days, 7 to 10 days, 8 to 11 days, 8 to 12, 9 to 13, 10 to 14, 11 to 15, 12 to 16, 13 to 17, 14 to 18, 15 to 19, or 16 to 20 days. In other embodiments, the mean half-life of a targeted binding agent or antibody as disclosed herein and of compositions of the invention is at least about 17 to 21 days, 18 to 22 days, 19 to 23 days, 20 to 24 days, 21 to 25, days, 22 to 26 days, 23 to 27 days, 24 to 28 days, 25 to 29 days, or 26 to 30 days. In still further embodiments the half-life of a targeted binding agent or antibody as disclosed herein and of compositions of the invention can be up to about 50 days. In certain embodiments, the half-lives of antibodies and of compositions of the invention can be prolonged by methods known in the art. Such prolongation can in turn reduce the amount and/or frequency of dosing of the antibody compositions. Antibodies with improved in vivo half-lives and methods for preparing them are disclosed in U.S. Pat. No. 6,277,375; and International Publication Nos. WO 98/23289 and WO 97/3461. 
     In another embodiment, the invention provides an article of manufacture including a container. The container includes a composition containing a targeted binding agent or antibody as disclosed herein, and a package insert or label indicating that the composition can be used to treat cell adhesion, invasion, angiogenesis, and/or proliferation-related diseases, including, but not limited to, diseases characterised by the expression or overexpression of KDR. 
     In other embodiments, the invention provides a kit comprising a composition containing a targeted binding agent or antibody as disclosed herein, and instructions to administer the composition to a subject in need of treatment. 
     The present invention provides formulation of proteins comprising a variant Fc region. That is, a non-naturally occurring Fc region, for example an Fc region comprising one or more non naturally occurring amino acid residues. Also encompassed by the variant Fc regions of present invention are Fc regions which comprise amino acid deletions, additions and/or modifications. 
     The serum half-life of proteins comprising Fc regions may be increased by increasing the binding affinity of the Fc region for FcRn. In one embodiment, the Fc variant protein has enhanced serum half life relative to comparable molecule. 
     In another embodiment, the present invention provides an Fc variant, wherein the Fc region comprises at least one non naturally occurring amino acid at one or more positions selected from the group consisting of 239, 330 and 332, as numbered by the EU index as set forth in Kabat. In a specific embodiment, the present invention provides an Fc variant, wherein the Fc region comprises at least one non naturally occurring amino acid selected from the group consisting of 239D, 330L and 332E, as numbered by the EU index as set forth in Kabat. Optionally, the Fc region may further comprise additional non naturally occurring amino acid at one or more positions selected from the group consisting of 252, 254, and 256, as numbered by the EU index as set forth in Kabat. In a specific embodiment, the present invention provides an Fc variant, wherein the Fc region comprises at least one non naturally occurring amino acid selected from the group consisting of 239D, 330L and 332E, as numbered by the EU index as set forth in Kabat and at least one non naturally occurring amino acid at one or more positions selected from the group consisting of 252Y, 254T and 256E, as numbered by the EU index as set forth in Kabat. 
     In another embodiment, the present invention provides an Fc variant, wherein the Fc region comprises at least one non naturally occurring amino acid at one or more positions selected from the group consisting of 234, 235 and 331, as numbered by the EU index as set forth in Kabat. In a specific embodiment, the present invention provides an Fc variant, wherein the Fc region comprises at least one non naturally occurring amino acid selected from the group consisting of 234F, 235F, 235Y, and 331S, as numbered by the EU index as set forth in Kabat. In a further specific embodiment, an Fc variant of the invention comprises the 234F, 235F, and 331S non naturally occurring amino acid residues, as numbered by the EU index as set forth in Kabat. In another specific embodiment, an Fc variant of the invention comprises the 234F, 235Y, and 331S non naturally occurring amino acid residues, as numbered by the EU index as set forth in Kabat. Optionally, the Fc region may further comprise additional non naturally occurring amino acid at one or more positions selected from the group consisting of 252, 254, and 256, as numbered by the EU index as set forth in Kabat. In a specific embodiment, the present invention provides an Fc variant, wherein the Fc region comprises at least one non naturally occurring amino acid selected from the group consisting of 234F, 235F, 235Y, and 331S, as numbered by the EU index as set forth in Kabat; and at least one non naturally occurring amino acid at one or more positions are selected from the group consisting of 252Y, 254T and 256E, as numbered by the EU index as set forth in Kabat. 
     In another embodiment, the present invention provides an Fc variant protein formulation, wherein the Fc region comprises at least a non naturally occurring amino acid at one or more positions selected from the group consisting of 239, 330 and 332, as numbered by the EU index as set forth in Kabat. In a specific embodiment, the present invention provides an Fc variant protein formulation, wherein the Fc region comprises at least one non naturally occurring amino acid selected from the group consisting of 239D, 330L and 332E, as numbered by the EU index as set forth in Kabat. Optionally, the Fc region may further comprise additional non naturally occurring amino acid at one or more positions selected from the group consisting of 252, 254, and 256, as numbered by the EU index as set forth in Kabat. In a specific embodiment, the present invention provides an Fc variant protein formulation, wherein the Fc region comprises at least one non naturally occurring amino acid selected from the group consisting of 239D, 330L and 332E, as numbered by the EU index as set forth in Kabat and at least one non naturally occurring amino acid at one or more positions are selected from the group consisting of 252Y, 254T and 256E, as numbered by the EU index as set forth in Kabat. 
     In another embodiment, the present invention provides an Fc variant protein formulation, wherein the Fc region comprises at least one non naturally occurring amino acid at one or more positions selected from the group consisting of 234, 235 and 331, as numbered by the EU index as set forth in Kabat. In a specific embodiment, the present invention provides an Fc variant protein formulation, wherein the Fc region comprises at least one non naturally occurring amino acid selected from the group consisting of 234F, 235F, 235Y, and 331S, as numbered by the EU index as set forth in Kabat. Optionally, the Fc region may further comprise additional non naturally occurring amino acid at one or more positions selected from the group consisting of 252, 254, and 256, as numbered by the EU index as set forth in Kabat. In a specific embodiment, the present invention provides an Fc variant protein formulation, wherein the Fc region comprises at least one non naturally occurring amino acid selected from the group consisting of 234F, 235F, 235Y, and 331S, as numbered by the EU index as set forth in Kabat; and at least one non naturally occurring amino acid at one or more positions are selected from the group consisting of 252Y, 254T and 256E, as numbered by the EU index as set forth in Kabat. 
     Methods for generating non naturally occurring Fc regions are known in the art. For example, amino acid substitutions and/or deletions can be generated by mutagenesis methods, including, but not limited to, site-directed mutagenesis (Kunkel,  Proc. Natl. Acad. Sci. USA  82:488-492 (1985)), PCR mutagenesis (Higuchi, in “PCR Protocols: A Guide to Methods and Applications”, Academic Press, San Diego, pp. 177-183 (1990)), and cassette mutagenesis (Wells et al., Gene 34:315-323 (1985)). Preferably, site-directed mutagenesis is performed by the overlap-extension PCR method (Higuchi, in “PCR Technology: Principles and Applications for DNA Amplification”, Stockton Press, New York, pp. 61-70 (1989)). The technique of overlap-extension PCR (Higuchi, ibid.) can also be used to introduce any desired mutation(s) into a target sequence (the starting DNA). For example, the first round of PCR in the overlap-extension method involves amplifying the target sequence with an outside primer (primer 1) and an internal mutagenesis primer (primer 3), and separately with a second outside primer (primer 4) and an internal primer (primer 2), yielding two PCR segments (segments A and B). The internal mutagenesis primer (primer 3) is designed to contain mismatches to the target sequence specifying the desired mutation(s). In the second round of PCR, the products of the first round of PCR (segments A and B) are amplified by PCR using the two outside primers (primers 1 and 4). The resulting full-length PCR segment (segment C) is digested with restriction enzymes and the resulting restriction fragment is cloned into an appropriate vector. As the first step of mutagenesis, the starting DNA (e.g., encoding an Fc fusion protein, an antibody or simply an Fc region), is operably cloned into a mutagenesis vector. The primers are designed to reflect the desired amino acid substitution. Other methods useful for the generation of variant Fc regions are known in the art (see, e.g., U.S. Pat. Nos. 5,624,821; 5,885,573; 5,677,425; 6,165,745; 6,277,375; 5,869,046; 6,121,022; 5,624,821; 5,648,260; 6,528,624; 6,194,551; 6,737,056; 6,821,505; 6,277,375; U.S. Patent Publication Nos. 2004/0002587 and PCT Publications WO 94/29351; WO 99/58572; WO 00/42072; WO 02/060919; WO 04/029207; WO 04/099249; WO 04/063351). 
     In some embodiments of the invention, the glycosylation patterns of the antibodies provided herein are modified to enhance ADCC and CDC effector function. See Shields R L et al., (2002) JBC. 277:26733; Shinkawa T et al., (2003) JBC. 278:3466 and Okazaki A et al., (2004) J. Mol. Biol., 336: 1239. In some embodiments, an Fc variant protein comprises one or more engineered glycoforms, i.e., a carbohydrate composition that is covalently attached to the molecule comprising an Fc region. Engineered glycoforms may be useful for a variety of purposes, including but not limited to enhancing or reducing effector function. Engineered glycoforms may be generated by any method known to one skilled in the art, for example by using engineered or variant expression strains, by co-expression with one or more enzymes, for example DI N-acetylglucosaminyltransferase III (GnTI11), by expressing a molecule comprising an Fc region in various organisms or cell lines from various organisms, or by modifying carbohydrate(s) after the molecule comprising Fc region has been expressed. Methods for generating engineered glycoforms are known in the art, and include but are not limited to those described in Umana et al, 1999, Nat. Biotechnol 17:176-180; Davies et al., 20017 Biotechnol Bioeng 74:288-294; Shields et al, 2002, J Biol Chem 277:26733-26740; Shinkawa et al., 2003, J Biol Chem 278:3466-3473) U.S. Pat. No. 6,602,684; U.S. Ser. No. 10/277,370; U.S. Ser. No. 10/113,929; PCT WO 00/61739A1; PCT WO 01/292246A1; PCT WO 02/311140A1; PCT WO 02/30954A1; Potillegent™ technology (Biowa, Inc. Princeton, N.J.); GlycoMAb™ glycosylation engineering technology (GLYCART biotechnology AG, Zurich, Switzerland). See, e.g., WO 00061739; EA01229125; US 20030115614; Okazaki et al., 2004, JMB, 336: 1239-49. 
     It is also known in the art that the glycosylation of the Fc region can be modified to increase or decrease effector function (see for examples, Umana et al, 1999, Nat. Biotechnol 17:176-180; Davies et al., 2001, Biotechnol Bioeng 74:288-294; Shields et al, 2002, J Biol Chem 277:26733-26740; Shinkawa et al., 2003, J Biol Chem 278:3466-3473) U.S. Pat. No. 6,602,684; U.S. Ser. No. 10/277,370; U.S. Ser. No. 10/113,929; PCT WO 00/61739A1; PCT WO 01/292246A1; PCT WO 02/311140A1; PCT WO 02/30954A1; Potillegent™ technology (Biowa, Inc. Princeton, N.J.); GlycoMAb™ glycosylation engineering technology (GLYCART biotechnology AG, Zurich, Switzerland). Accordingly, in one embodiment the Fc regions of the antibodies of the invention comprise altered glycosylation of amino acid residues. In another embodiment, the altered glycosylation of the amino acid residues results in lowered effector function. In another embodiment, the altered glycosylation of the amino acid residues results in increased effector function. In a specific embodiment, the Fc region has reduced fucosylation. In another embodiment, the Fc region is afucosylated (see for examples, U.S. Patent Application Publication No. 2005/0226867). 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a bar chart showing the effect of inhibitory KDR antibodies on endothelial cell tube formation in a vessel length endothelial tube formation assay. Antibodies are indicated on the X axis and concentrations from left to right in each group of bars are 20 μg/mL, 5 μg/mL, 1.25 μg/mL and 0.3125 μg/mL. 
         FIG. 2  is a bar chart showing the effect of inhibitory KDR antibodies on angiogenesis in vivo. Along the X axis 1=vehicle twice weekly; 2=24B3 10 mg/kg twice weekly; 3=24B3 1 mg/kg twice weekly; 4=33C3 10 mg/kg twice weekly; 5=33C3 1 mg/kg twice weekly. The Y axis shows vessel numbers. 
     
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT 
     Embodiments of the invention relate to a novel set of VEGFR blocking molecules, such as, for example, antibodies, that inhibit VEGFR signaling without blocking binding of ligand to its receptor. Such molecules can be used as single agents, or alternatively, in combination with VEGF-A binding antibodies/agents, antibodies that inhibit receptor-ligand binding and small molecule inhibitors of VEGFRs. They can also be used in combination with any standard or novel anti-cancer agents. 
     Embodiments of the invention relate to targeted binding agents that bind to KDR. In some embodiments, the targeted binding agents bind to KDR and inhibit the binding of the protein product of vascular endothelial growth factor (VEGF) to KDR. In some embodiments, the targeted binding agents bind to KDR and inhibit receptor dimerisation. In some embodiments, the targeted binding agents bind to KDR and inhibit receptor dimerisation and binding of VEGF to KDR. In one embodiment, the targeted binding agents are monoclonal antibodies, or binding fragments thereof. Such monoclonal antibodies may be referred to as anti-KDR antibodies herein. 
     Other embodiments of the invention include fully human anti-KDR antibodies, and antibody preparations that are therapeutically useful. In one embodiment, preparations of the anti-KDR antibody of the invention have desirable therapeutic properties, including strong binding affinity for KDR, the ability to inhibit KDR tyrosine phosphorylation in vitro, and the ability to inhibit KDR-induced cell activity in vitro and in vivo. 
     In addition, embodiments of the invention include methods of using these antibodies for treating diseases. Anti-KDR antibodies of the invention are useful for preventing KDR-mediated tumourigenesis and tumour invasion of healthy tissue. In addition KDR antibodies can be useful for treating diseases associated with angiogenesis such as ocular disease such as AMD, inflammatory disorders such as rheumatoid arthritis, and cardiovascular disease and sepsis as well as neoplastic diseases. While not being limited to any particular theory, the mechanism of action of this inhibition can include inhibition of VEGF from binding to KDR and/or by inhibiting dimerisation of the receptor, thereby preventing productive signaling and activation of proliferative signals. Diseases that are treatable through this inhibition mechanism include, but are not limited to a neoplastic disease. Any disease that is characterized by any type of malignant tumour, including metastatic cancers, lymphatic tumours, and blood cancers, can also be treated by this inhibition mechanism. Exemplary cancers in humans include a bladder tumour, breast tumour, prostate tumour, basal cell carcinoma, biliary tract cancer, bladder cancer, bone cancer, brain and CNS cancer (e.g., glioma tumour), cervical cancer, choriocarcinoma, colon and rectum cancer, connective tissue cancer, cancer of the digestive system; endometrial cancer, esophageal cancer; eye cancer; cancer of the head and neck; gastric cancer; intra-epithelial neoplasm; kidney cancer; larynx cancer; leukemia; liver cancer; lung cancer (e.g. small cell and non-small cell); lymphoma including Hodgkin&#39;s and Non-Hodgkin&#39;s lymphoma; melanoma; myeloma, neuroblastoma, oral cavity cancer (e.g., lip, tongue, mouth, and pharynx); ovarian cancer; pancreatic cancer, retinoblastoma; rhabdomyosarcoma; rectal cancer, renal cancer, cancer of the respiratory system; sarcoma, skin cancer; stomach cancer, testicular cancer, thyroid cancer; uterine cancer, cancer of the urinary system, as well as other carcinomas and sarcomas. Malignant disorders commonly diagnosed in dogs, cats, and other pets include, but are not limited to, lymphosarcoma, osteosarcoma, mammary tumours, mastocytoma, brain tumour, melanoma, adenosquamous carcinoma, carcinoid lung tumour, bronchial gland tumour, bronchiolar adenocarcinoma, fibroma, myxochondroma, pulmonary sarcoma, neurosarcoma, osteoma, papilloma, retinoblastoma, Ewing&#39;s sarcoma, Wilm&#39;s tumour, Burkitt&#39;s lymphoma, microglioma, neuroblastoma, osteoclastoma, oral neoplasia, fibrosarcoma, osteosarcoma and rhabdomyosarcoma, genital squamous cell carcinoma, transmissible venereal tumour, testicular tumour, seminoma, Sertoli cell tumour, hemangiopericytoma, histiocytoma, chloroma (e.g., granulocytic sarcoma), corneal papilloma, corneal squamous cell carcinoma, hemangiosarcoma, pleural mesothelioma, basal cell tumour, thymoma, stomach tumour, adrenal gland carcinoma, oral papillomatosis, hemangioendothelioma and cystadenoma, follicular lymphoma, intestinal lymphosarcoma, fibrosarcoma and pulmonary squamous cell carcinoma. In rodents, such as a ferret, exemplary cancers include insulinoma, lymphoma, sarcoma, neuroma, pancreatic islet cell tumour, gastric MALT lymphoma and gastric adenocarcinoma. Neoplasias affecting agricultural livestock include leukemia, hemangiopericytoma and bovine ocular neoplasia (in cattle); preputial fibrosarcoma, ulcerative squamous cell carcinoma, preputial carcinoma, connective tissue neoplasia and mastocytoma (in horses); hepatocellular carcinoma (in swine); lymphoma and pulmonary adenomatosis (in sheep); pulmonary sarcoma, lymphoma, Rous sarcoma, reticulo-endotheliosis, fibrosarcoma, nephroblastoma, B-cell lymphoma and lymphoid leukosis (in avian species); retinoblastoma, hepatic neoplasia, lymphosarcoma (lymphoblastic lymphoma), plasmacytoid leukemia and swimbladder sarcoma (in fish), caseous lumphadenitis (CLA): chronic, infectious, contagious disease of sheep and goats caused by the bacterium  Corynebacterium pseudotuberculosis , and contagious lung tumour of sheep caused by jaagsiekte. 
     Other embodiments of the invention include diagnostic assays for specifically determining the quantity of KDR in a biological sample. The assay kit can include a targeted binding agent or antibody as disclosed herein along with the necessary labels for detecting such antibodies. These diagnostic assays are useful to screen for cell adhesion, invasion, angiogenesis or proliferation-related diseases including, but not limited to, neoplastic diseases. 
     Another aspect of the invention is an antagonist of the biological activity of KDR wherein the antagonist binds to KDR. In one embodiment, the antagonist is a targeted binding agent, such as an antibody. The antagonist may bind to:
         i) KDR; or   ii) the KDR/VEGF complex,
 
or a combination of these. In one embodiment the antagonist is able to antagonize the biological activity of KDR in vitro and in vivo. The antagonist may be selected from an antibody described herein, for example, antibody 27D10, 24B3 or 33C3.
       

     In one embodiment the antagonist of the biological activity of KDR may bind to KDR and thereby inhibit or suppress KDR receptor tyrosine kinase activity, thereby inhibiting cell adhesion and/or invasion and/or angiogenesis and/or proliferation. The mechanism of action of this inhibition may include binding of the antagonist to KDR and inhibiting the binding of a native KDR-specific ligand, such as, for example VEGF, to KDR. The mechanism of action of this inhibition may include binding of the antagonist to KDR and inhibiting dimerisation of KDR. Without wishing to be bound by any particular theoretical considerations, mechanisms by which antagonism of the biological activity of KDR can be achieved include, but are not limited to, inhibition of binding of VEGF to KDR, and/or inhibition of receptor dimerisation or inhibition of KDR-VEGF mediated signaling activity. 
     One embodiment is a targeted binding agent which binds to the same epitope or epitopes as fully human monoclonal antibody 27D10, 24B3 or 33C3. 
     One embodiment is an antibody which binds to the same epitope or epitopes as fully human monoclonal antibody 27D10, 24B3 or 33C3. 
     One embodiment is a hybridoma that produces the targeted binding agent as described hereinabove. In one embodiment is a hybridoma that produces the light chain and/or the heavy chain of the antibodies as described hereinabove. In one embodiment the hybridoma produces the light chain and/or the heavy chain of a fully human monoclonal antibody. In another embodiment the hybridoma produces the light chain and/or the heavy chain of fully human monoclonal antibody 27D10, 24B3 or 33C3. Alternatively the hybridoma may produce an antibody which binds to the same epitope or epitopes as fully human monoclonal antibody 27D10, 24B3 or 33C3. 
     Another embodiment is a nucleic acid molecule encoding the targeted binding agent as described hereinabove. In one embodiment is a nucleic acid molecule encoding the light chain or the heavy chain of an antibody as described hereinabove. In one embodiment the nucleic acid molecule encodes the light chain or the heavy chain of a fully human monoclonal antibody. Still another embodiment is a nucleic acid molecule encoding the light chain or the heavy chain of a fully human monoclonal antibody selected from antibodies 27D10, 24B3 or 33C3. 
     Another embodiment of the invention is a vector comprising a nucleic acid molecule or molecules as described hereinabove, wherein the vector encodes a targeted binding agent as defined hereinabove. In one embodiment of the invention is a vector comprising a nucleic acid molecule or molecules as described hereinabove, wherein the vector encodes a light chain and/or a heavy chain of an antibody as defined hereinabove. 
     Yet another embodiment of the invention is a host cell comprising a vector as described hereinabove. Alternatively the host cell may comprise more than one vector. 
     In addition, one embodiment of the invention is a method of producing a targeted binding agent of the invention by culturing host cells under conditions wherein a nucleic acid molecule is expressed to produce the targeted binding agent, followed by recovery of the targeted binding agent. In one embodiment of the invention is a method of producing an antibody of the invention by culturing host cells under conditions wherein a nucleic acid molecule is expressed to produce the antibody, followed by recovery of the antibody. 
     In one embodiment the invention includes a method of making an targeted binding agent by transfecting at least one host cell with at least one nucleic acid molecule encoding the targeted binding agent as described hereinabove, expressing the nucleic acid molecule in the host cell and isolating the targeted binding agent. In one embodiment the invention includes a method of making an antibody by transfecting at least one host cell with at least one nucleic acid molecule encoding the antibody as described hereinabove, expressing the nucleic acid molecule in the host cell and isolating the antibody. 
     According to another aspect, the invention includes a method of antagonising the biological activity of KDR by administering an antagonist as described herein. The method may include selecting an animal in need of treatment for disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation, and administering to the animal a therapeutically effective dose of an antagonist of the biological activity of KDR. 
     Another aspect of the invention includes a method of antagonising the biological activity of KDR by administering a targeted binding agent as described hereinabove. The method may include selecting an animal in need of treatment for disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation, and administering to the animal a therapeutically effective dose of a targeted binding agent which antagonises the biological activity of KDR. 
     Another aspect of the invention includes a method of antagonising the biological activity of KDR by administering an antibody as described hereinabove. The method may include selecting an animal in need of treatment for disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation, and administering to the animal a therapeutically effective dose of an antibody which antagonises the biological activity of KDR. 
     According to another aspect there is provided a method of treating disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation in an animal by administering a therapeutically effective amount of an antagonist of the biological activity of KDR. The method may include selecting an animal in need of treatment for disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation, and administering to the animal a therapeutically effective dose of an antagonist of the biological activity of KDR. 
     According to another aspect there is provided a method of treating disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation in an animal by administering a therapeutically effective amount of a targeted binding agent which antagonizes the biological activity of KDR. The method may include selecting an animal in need of treatment for disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation, and administering to the animal a therapeutically effective dose of a targeted binding agent which antagonises the biological activity of KDR. The targeted binding agent can be administered alone, or can be administered in combination with additional antibodies or chemotherapeutic drugs or radiation therapy. 
     According to another aspect there is provided a method of treating disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation in an animal by administering a therapeutically effective amount of an antibody which antagonizes the biological activity of KDR. The method may include selecting an animal in need of treatment for disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation, and administering to the animal a therapeutically effective dose of an antibody which antagonises the biological activity of KDR. The antibody can be administered alone, or can be administered in combination with additional antibodies or chemotherapeutic drugs or radiation therapy. 
     According to another aspect there is provided a method of treating cancer in an animal by administering a therapeutically effective amount of an antagonist of the biological activity of KDR. The method may include selecting an animal in need of treatment for cancer, and administering to the animal a therapeutically effective dose of an antagonist which antagonises the biological activity of KDR. The antagonist can be administered alone, or can be administered in combination with additional antibodies or chemotherapeutic drugs or radiation therapy. 
     According to another aspect there is provided a method of treating cancer in an animal by administering a therapeutically effective amount of a targeted binding agent which antagonizes the biological activity of KDR. The method may include selecting an animal in need of treatment for cancer, and administering to the animal a therapeutically effective dose of a targeted binding agent which antagonises the biological activity of KDR. The targeted binding agent can be administered alone, or can be administered in combination with additional antibodies or chemotherapeutic drugs or radiation therapy. 
     According to another aspect there is provided a method of treating cancer in an animal by administering a therapeutically effective amount of an antibody which antagonizes the biological activity of KDR. The method may include selecting an animal in need of treatment for cancer, and administering to the animal a therapeutically effective dose of an antibody which antagonises the biological activity of KDR. The antibody can be administered alone, or can be administered in combination with additional antibodies or chemotherapeutic drugs or radiation therapy. 
     According to another aspect there is provided a method of reducing or inhibiting tumour cell proliferation, adhesion, invasion and/or angiogenesis, in an animal by administering a therapeutically effective amount of an antibody which antagonizes the biological activity of KDR. The method may include selecting an animal in need of a reduction or inhibition of proliferation, cell adhesion, invasion and/or angiogenesis, and administering to the animal a therapeutically effective dose of an antibody which antagonises the biological activity of KDR. The antibody can be administered alone, or can be administered in combination with additional antibodies or chemotherapeutic drugs or radiation therapy. 
     According to another aspect there is provided a method of reducing tumour growth and/or metastasis, in an animal by administering a therapeutically effective amount of an antibody which antagonizes the biological activity of KDR. The method may include selecting an animal in need of a reduction of tumour growth and/or metastasis, and administering to the animal a therapeutically effective dose of an antibody which antagonises the biological activity of KDR. The antibody can be administered alone, or can be administered in combination with additional antibodies or chemotherapeutic drugs or radiation therapy. 
     According to another aspect of the invention there is provided the use of an antagonist of the biological activity of KDR for the manufacture of a medicament for the treatment of disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation. In one embodiment the antagonist of the biological activity of KDR is a targeted binding agent of the invention. In one embodiment the antagonist of the biological activity of KDR is an antibody of the invention. 
     According to another aspect of the invention there is provided an antagonist of the biological activity of KDR for use as a medicament for the treatment of disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation. In one embodiment the antagonist of the biological activity of KDR is a targeted binding agent of the invention. In one embodiment the antagonist of the biological activity of KDR is an antibody of the invention. 
     According to another aspect of the invention there is provided the use of a targeted binding agent or an antibody which antagonizes the biological activity of KDR for the manufacture of a medicament for the treatment of disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation. 
     According to another aspect of the invention there is provided a targeted binding agent or an antibody which antagonizes the biological activity of KDR for use as a medicament for the treatment of disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation. 
     According to another aspect of the invention there is provided the use of a targeted binding agent or an antibody which antagonizes the biological activity of KDR for the manufacture of a medicament for the treatment of disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation. 
     According to another aspect of the invention there is provided an antibody which antagonizes the biological activity of KDR for use as a medicament for the treatment of disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation. 
     According to another aspect of the invention there is provided the use of an antagonist of the biological activity of KDR for the manufacture of a medicament for the treatment of cancer in a mammal In one embodiment the antagonist of the biological activity of KDR is a targeted binding agent of the invention. In one embodiment the antagonist of the biological activity of KDR is an antibody of the invention. 
     According to another aspect of the invention there is provided an antagonist of the biological activity of KDR for use as a medicament for the treatment of cancer in a mammal. In one embodiment the antagonist of the biological activity of KDR is a targeted binding agent of the invention. In one embodiment the antagonist of the biological activity of KDR is an antibody of the invention. 
     According to another aspect of the invention there is provided the use of a targeted binding agent which antagonizes the biological activity of KDR for the manufacture of a medicament for the treatment of cancer in a mammal. 
     According to another aspect of the invention there is provided a targeted binding agent which antagonizes the biological activity of KDR for use as a medicament for the treatment of cancer in a mammal. 
     According to another aspect of the invention there is provided the use of an antibody which antagonizes the biological activity of KDR for the manufacture of a medicament for the treatment of cancer in a mammal. 
     According to another aspect of the invention there is provided an antibody which antagonizes the biological activity of KDR for use as a medicament for the treatment of cancer in a mammal. 
     According to another aspect there is provided the use of a targeted binding agent or an antibody which antagonizes the biological activity of KDR for the manufacture of a medicament for the reduction or inhibition proliferation, cell adhesion, invasion and/or angiogenesis in an animal. 
     According to another aspect there is provided a targeted binding agent or an antibody which antagonizes the biological activity of KDR for use as a medicament for the reduction or inhibition proliferation, cell adhesion, invasion and/or angiogenesis in an animal. 
     According to another aspect there is provided the use of a targeted binding agent or an antibody which antagonizes the biological activity of KDR for the manufacture of a medicament for reducing tumour growth and/or metastasis, in an animal. 
     According to another aspect there is provided a targeted binding agent or an antibody which antagonizes the biological activity of KDR for use as a medicament for reducing tumour growth and/or metastasis, in an animal. 
     In one embodiment the present invention is particularly suitable for use in antagonizing KDR, in patients with a tumour which is dependent alone, or in part, on KDR receptor tyrosine kinase. 
     According to another aspect of the invention there is provided a pharmaceutical composition comprising an antagonist of the biological activity of KDR, and a pharmaceutically acceptable carrier. In one embodiment the antagonist comprises an antibody. According to another aspect of the invention there is provided a pharmaceutical composition comprising an antagonist of the biological activity of KDR, and a pharmaceutically acceptable carrier. In one embodiment the antagonist comprises an antibody. 
     In some embodiments, following administration of the antibody that specifically binds to KDR, a clearing agent is administered, to remove excess circulating antibody from the blood. 
     Anti-KDR antibodies are useful in the detection of KDR in patient samples and accordingly are useful as diagnostics for disease states as described herein. In addition, based on their ability to significantly inhibit KDR-mediated signaling activity (as demonstrated in the Examples below), anti-KDR antibodies have therapeutic effects in treating symptoms and conditions resulting from KDR expression. In specific embodiments, the antibodies and methods herein relate to the treatment of symptoms resulting from KDR induced cell adhesion, invasion, angiogenesis, proliferation and/or intracellular signaling. Further embodiments involve using the antibodies and methods described herein to treat cell adhesion, invasion, angiogenesis and/or proliferation-related diseases including neoplastic diseases, such as, melanoma, small cell lung cancer, non-small cell lung cancer, glioma, hepatocellular (liver) carcinoma, thyroid tumour, gastric (stomach) cancer, prostate cancer, breast cancer, ovarian cancer, bladder cancer, lung cancer, glioblastoma, endometrial cancer, kidney cancer, colon cancer, and pancreatic cancer. The antibodies may also be useful in treating cell adhesion and/or invasion in arthritis, atherosclerosis and diseases involving angiogenesis. 
     Another embodiment of the invention includes an assay kit for detecting KDR in mammalian tissues, cells, or body fluids to screen for cell adhesion-, invasion-, angiogenesis- or proliferation related diseases. The kit includes a targeted binding agent that binds to KDR and a means for indicating the reaction of the targeted binding agent with KDR, if present. In one embodiment, the targeted binding agent that binds KDR is labeled. In another embodiment the targeted binding agent is an unlabeled and the kit further includes a means for detecting the targeted binding agent. Preferably the targeted binding agent is labeled with a marker selected from the group consisting of a fluorochrome, an enzyme, a radionuclide and a radio-opaque material. 
     Another embodiment of the invention includes an assay kit for detecting KDR in mammalian tissues, cells, or body fluids to screen for cell adhesion-, invasion-, angiogenesis or proliferation-related diseases. The kit includes an antibody that binds to KDR and a means for indicating the reaction of the antibody with KDR, if present. The antibody may be a monoclonal antibody. In one embodiment, the antibody that binds KDR is labeled. In another embodiment the antibody is an unlabeled primary antibody and the kit further includes a means for detecting the primary antibody. In one embodiment, the means includes a labeled second antibody that is an anti-immunoglobulin. Preferably the antibody is labeled with a marker selected from the group consisting of a fluorochrome, an enzyme, a radionuclide and a radio-opaque material. 
     Further embodiments, features, and the like regarding the antibodies as disclosed herein are provided in additional detail below. 
     Sequence Listing 
     Embodiments of the invention include the specific antibodies listed below in Table 1. This table reports the identification number of each anti-KDR antibody, along with the SEQ ID number of the variable domain of the corresponding heavy chain and light chain genes and polypeptides, respectively. Each antibody has been given an identification number. 
                             TABLE 1               MAb ID       SEQ ID       No.:   Sequence   NO:                                            30D7   Nucleotide sequence encoding the variable region of the heavy chain   1           Amino acid sequence of the variable region of the heavy chain   2           Nucleotide sequence encoding the variable region of the light chain   3           Amino acid sequence of the variable region of the light chain   4       21H9   Nucleotide sequence encoding the variable region of the heavy chain   5           Amino acid sequence of the variable region of the heavy chain   6           Nucleotide sequence encoding the variable region of the light chain   7           Amino acid sequence of the variable region of the light chain   8       27A3   Nucleotide sequence encoding the variable region of the heavy chain   9           Amino acid sequence of the variable region of the heavy chain   10           Nucleotide sequence encoding the variable region of the light chain   11           Amino acid sequence of the variable region of the light chain   12       27D10   Nucleotide sequence encoding the variable region of the heavy chain   13           Amino acid sequence of the variable region of the heavy chain   14           Nucleotide sequence encoding the variable region of the light chain   15           Amino acid sequence of the variable region of the light chain   16       32F4   Nucleotide sequence encoding the variable region of the heavy chain   17           Amino acid sequence of the variable region of the heavy chain   18           Nucleotide sequence encoding the variable region of the light chain   19           Amino acid sequence of the variable region of the light chain   20       29D4   Nucleotide sequence encoding the variable region of the heavy chain   21           Amino acid sequence of the variable region of the heavy chain   22           Nucleotide sequence encoding the variable region of the light chain   23           Amino acid sequence of the variable region of the light chain   24       30A1   Nucleotide sequence encoding the variable region of the heavy chain   25           Amino acid sequence of the variable region of the heavy chain   26           Nucleotide sequence encoding the variable region of the light chain   27           Amino acid sequence of the variable region of the light chain   28       22B8   Nucleotide sequence encoding the variable region of the heavy chain   29           Amino acid sequence of the variable region of the heavy chain   30           Nucleotide sequence encoding the variable region of the light chain   31           Amino acid sequence of the variable region of the light chain   32       24C9   Nucleotide sequence encoding the variable region of the heavy chain   33           Amino acid sequence of the variable region of the heavy chain   34           Nucleotide sequence encoding the variable region of the light chain   35           Amino acid sequence of the variable region of the light chain   36       32G7   Nucleotide sequence encoding the variable region of the heavy chain   37           Amino acid sequence of the variable region of the heavy chain   38           Nucleotide sequence encoding the variable region of the light chain   39           Amino acid sequence of the variable region of the light chain   40       24B3   Nucleotide sequence encoding the variable region of the heavy chain   41           Amino acid sequence of the variable region of the heavy chain   42           Nucleotide sequence encoding the variable region of the light chain   43           Amino acid sequence of the variable region of the light chain   44       33B1   Nucleotide sequence encoding the variable region of the heavy chain   45           Amino acid sequence of the variable region of the heavy chain   46           Nucleotide sequence encoding the variable region of the light chain   47           Amino acid sequence of the variable region of the light chain   48       33E1   Nucleotide sequence encoding the variable region of the heavy chain   49           Amino acid sequence of the variable region of the heavy chain   50           Nucleotide sequence encoding the variable region of the light chain   51           Amino acid sequence of the variable region of the light chain   52       29H3   Nucleotide sequence encoding the variable region of the heavy chain   53           Amino acid sequence of the variable region of the heavy chain   54           Nucleotide sequence encoding the variable region of the light chain   55           Amino acid sequence of the variable region of the light chain   56       33D5   Nucleotide sequence encoding the variable region of the heavy chain   57           Amino acid sequence of the variable region of the heavy chain   58           Nucleotide sequence encoding the variable region of the light chain   59           Amino acid sequence of the variable region of the light chain   60       29F7   Nucleotide sequence encoding the variable region of the heavy chain   61           Amino acid sequence of the variable region of the heavy chain   62           Nucleotide sequence encoding the variable region of the light chain   63           Amino acid sequence of the variable region of the light chain   64       21A1   Nucleotide sequence encoding the variable region of the heavy chain   65           Amino acid sequence of the variable region of the heavy chain   66           Nucleotide sequence encoding the variable region of the light chain   67           Amino acid sequence of the variable region of the light chain   68       31E11   Nucleotide sequence encoding the variable region of the heavy chain   69           Amino acid sequence of the variable region of the heavy chain   70           Nucleotide sequence encoding the variable region of the light chain   71           Amino acid sequence of the variable region of the light chain   72       33C3   Nucleotide sequence encoding the variable region of the heavy chain   73           Amino acid sequence of the variable region of the heavy chain   74           Nucleotide sequence encoding the variable region of the light chain   75           Amino acid sequence of the variable region of the light chain   76       30F6   Nucleotide sequence encoding the variable region of the heavy chain   77           Amino acid sequence of the variable region of the heavy chain   78           Nucleotide sequence encoding the variable region of the light chain   79           Amino acid sequence of the variable region of the light chain   80       32B2   Nucleotide sequence encoding the variable region of the heavy chain   81           Amino acid sequence of the variable region of the heavy chain   82           Nucleotide sequence encoding the variable region of the light chain   83           Amino acid sequence of the variable region of the light chain   84       30E3   Nucleotide sequence encoding the variable region of the heavy chain   85           Amino acid sequence of the variable region of the heavy chain   86           Nucleotide sequence encoding the variable region of the light chain   87           Amino acid sequence of the variable region of the light chain   88       29A11   Nucleotide sequence encoding the variable region of the heavy chain   89           Amino acid sequence of the variable region of the heavy chain   90           Nucleotide sequence encoding the variable region of the light chain   91           Amino acid sequence of the variable region of the light chain   92       30H10   Nucleotide sequence encoding the variable region of the heavy chain   93           Amino acid sequence of the variable region of the heavy chain   94           Nucleotide sequence encoding the variable region of the light chain   95           Amino acid sequence of the variable region of the light chain   96       32C11   Nucleotide sequence encoding the variable region of the heavy chain   97           Amino acid sequence of the variable region of the heavy chain   98           Nucleotide sequence encoding the variable region of the light chain   99           Amino acid sequence of the variable region of the light chain   100       29A3   Nucleotide sequence encoding the variable region of the heavy chain   101           Amino acid sequence of the variable region of the heavy chain   102           Nucleotide sequence encoding the variable region of the light chain   103           Amino acid sequence of the variable region of the light chain   104       1G6   Nucleotide sequence encoding the variable region of the heavy chain   105           Amino acid sequence of the variable region of the heavy chain   131           Nucleotide sequence encoding the variable region of the light chain   107           Amino acid sequence of the variable region of the light chain   108                    
Definitions
 
     Unless otherwise defined, scientific and technical terms used herein shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular. Generally, nomenclatures utilized in connection with, and techniques of, cell and tissue culture, molecular biology, and protein and oligo- or polynucleotide chemistry and hybridization described herein are those well known and commonly used in the art. 
     Standard techniques are used for recombinant DNA, oligonucleotide synthesis, and tissue culture and transformation (e.g., electroporation, lipofection). Enzymatic reactions and purification techniques are performed according to manufacturer&#39;s specifications or as commonly accomplished in the art or as described herein. The foregoing techniques and procedures are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification. See e.g., Sambrook et al.  Molecular Cloning: A Laboratory Manual  (3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2001)), which is incorporated herein by reference. The nomenclatures utilized in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those well known and commonly used in the art. Standard techniques are used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients. 
     As utilized in accordance with the present disclosure, the following terms, unless otherwise indicated, shall be understood to have the following meanings: 
     An antagonist or inhibitor may be a polypeptide, nucleic acid, carbohydrate, lipid, small molecular weight compound, an oligonucleotide, an oligopeptide, RNA interference (RNAi), antisense, a recombinant protein, an antibody, or fragments thereof or conjugates or fusion proteins thereof. For a review of RNAi see Milhavet O, Gary D S, Mattson M P. (Pharmacol Rev. 2003 December; 55(4):629-48. Review) and antisense (see Opalinska J B, Gewirtz A M. (Sci STKE. 2003 Oct. 28; 2003 (206):pe47.) 
     Disease-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation may be any abnormal, undesirable or pathological cell adhesion and/or invasion and/or angiogenesis and/or proliferation, for example tumour-related cell adhesion and/or invasion and/or angiogenesis and/or proliferation. Cell adhesion- and/or invasion and/or angiogenesis- and/or proliferation-related diseases include, but are not limited to, non-solid tumours such as leukemia, multiple myeloma or lymphoma, and also solid tumours such as melanoma, small cell lung cancer, non-small cell lung cancer, glioma, hepatocellular (liver) carcinoma, glioblastoma, carcinoma of the thyroid, bile duct, bone, gastric, brain/CNS, head and neck, hepatic system, stomach, prostate, breast, renal, testicle, ovary, skin, cervix, lung, muscle, neuron, esophageal, bladder, lung, uterus, vulva, endometrium, kidney, colorectum, pancreas, pleural/peritoneal membranes, salivary gland, and epidermous. 
     A compound refers to any small molecular weight compound with a molecular weight of less than about 2000 Daltons. 
     The term “KDR” refers to the molecule that is KDR protein, also known as VEGFR2 and KDR receptor-like tyrosine kinase. 
     The terms “neutralizing” or “inhibits” when referring to a targeted binding agent, such as an antibody, relates to the ability of an antibody to eliminate, reduce, or significantly reduce, the activity of a target antigen. Accordingly, a “neutralizing” anti-KDR antibody of the invention is capable of eliminating or significantly reducing the activity of KDR. A neutralizing KDR antibody may, for example, act by blocking the binding of a native KDR-specific ligand, such as, for example, VEGF, to KDR. By blocking this binding, KDR signal-mediated activity is significantly, or completely, eliminated. Ideally, a neutralizing antibody against KDR inhibits cell adhesion and/or invasion and/or angiogenesis and/or proliferation. 
     An “antagonist of the biological activity of KDR” is capable of eliminating, reducing or significantly reducing the activity of KDR. An “antagonist of the biological activity of KDR” is capable of eliminating, reducing or significantly reducing KDR signaling. An “antagonist of the biological activity of KDR” may eliminate or significantly reduce cell adhesion and/or invasion and/or angiogenesis and/or proliferation. 
     “Reducing KDR signaling” encompasses a reduction of KDR signaling by at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% in comparison with the level of signaling in the absence of a targeted binding agent, antibody or antagonist of the invention. 
     The term “polypeptide” is used herein as a generic term to refer to native protein, fragments, or analogs of a polypeptide sequence. Hence, native protein, fragments, and analogs are species of the polypeptide genus. Preferred polypeptides in accordance with the invention comprise the human heavy chain immunoglobulin molecules and the human kappa light chain immunoglobulin molecules, as well as antibody molecules formed by combinations comprising the heavy chain immunoglobulin molecules with light chain immunoglobulin molecules, such as the kappa or lambda light chain immunoglobulin molecules, and vice versa, as well as fragments and analogs thereof. Preferred polypeptides in accordance with the invention may also comprise solely the human heavy chain immunoglobulin molecules or fragments thereof. 
     The terms “native” or “naturally-occurring” as used herein as applied to an object refers to the fact that an object can be found in nature. For example, a polypeptide or polynucleotide sequence that is present in an organism (including viruses) that can be isolated from a source in nature and which has not been intentionally modified by man in the laboratory or otherwise is naturally-occurring. 
     The term “operably linked” as used herein refers to positions of components so described that are in a relationship permitting them to function in their intended manner. For example, a control sequence “operably linked” to a coding sequence is connected in such a way that expression of the coding sequence is achieved under conditions compatible with the control sequences. 
     The term “polynucleotide” as referred to herein means a polymeric form of nucleotides of at least 10 bases in length, either ribonucleotides or deoxynucleotides or a modified form of either type of nucleotide, or RNA-DNA hetero-duplexes. The term includes single and double stranded forms of DNA. 
     The term “oligonucleotide” referred to herein includes naturally occurring, and modified nucleotides linked together by naturally occurring, and non-naturally occurring linkages. Oligonucleotides are a polynucleotide subset generally comprising a length of 200 bases or fewer. Preferably, oligonucleotides are 10 to 60 bases in length and most preferably 12, 13, 14, 15, 16, 17, 18, 19, or 20 to 40 bases in length. Oligonucleotides are usually single stranded, e.g. for probes; although oligonucleotides may be double stranded, e.g. for use in the construction of a gene mutant. Oligonucleotides can be either sense or antisense oligonucleotides. 
     The term “naturally occurring nucleotides” referred to herein includes deoxyribonucleotides and ribonucleotides. The term “modified nucleotides” referred to herein includes nucleotides with modified or substituted sugar groups and the like. The term “oligonucleotide linkages” referred to herein includes oligonucleotides linkages such as phosphorothioate, phosphorodithioate, phosphoroselenoate, phosphorodiselenoate, phosphoroanilothioate, phosphoraniladate, phosphoroamidate, and the like. See e.g., LaPlanche et al.  Nucl. Acids Res.  14:9081 (1986); Stec et al.  J. Am. Chem. Soc.  106:6077 (1984); Stein et al.  Nucl. Acids Res.  16:3209 (1988); Zon et al.  Anti - Cancer Drug Design  6:539 (1991); Zon et al.  Oligonucleotides and Analogues: A Practical Approach , pp. 87-108 (F. Eckstein, Ed., Oxford University Press, Oxford England (1991)); Stec et al. U.S. Pat. No. 5,151,510; Uhlmann and Peyman  Chemical Reviews  90:543 (1990), the disclosures of which are hereby incorporated by reference. An oligonucleotide can include a label for detection, if desired. 
     The term “selectively hybridise” referred to herein means to detectably and specifically bind. Polynucleotides, oligonucleotides and fragments thereof selectively hybridise to nucleic acid strands under hybridisation and wash conditions that minimise appreciable amounts of detectable binding to nonspecific nucleic acids. High stringency conditions can be used to achieve selective hybridisation conditions as known in the art and discussed herein. Generally, the nucleic acid sequence homology between the polynucleotides, oligonucleotides, or antibody fragments and a nucleic acid sequence of interest will be at least 80%, and more typically with preferably increasing homologies of at least 85%, 90%, 95%, 99%, and 100%. 
     Stringent hybridization conditions include, but are not limited to, hybridization to filter-bound DNA in 6× sodium chloride/sodium citrate (SSC) (0.9 M NaCl/90 mM NaCitrate, pH 7.0) at about 45° C. followed by one or more washes in 0.2×SSC/0.1% SDS at about 50-65° C., highly stringent conditions such as hybridization to filter-bound DNA in 6×SSC at about 45° C. followed by one or more washes in 0.1×SSC/0.2% SDS at about 60° C., or any other stringent hybridization conditions known to those skilled in the art (see, for example, Ausubel, F. M. et al.,  eds. 1989 Current Protocols in Molecular Biology, vol. 1, Green Publishing Associates, Inc. and John Wiley and Sons, Inc., NY at pages 6.3.1 to 6.3.6 and 2.10.3). Two amino acid sequences are “homologous” if there is a partial or complete identity between their sequences. For example, 85% homology means that 85% of the amino acids are identical when the two sequences are aligned for maximum matching. Gaps (in either of the two sequences being matched) are allowed in maximizing matching; gap lengths of 5 or less are preferred with 2 or less being more preferred. Alternatively and preferably, two protein sequences (or polypeptide sequences derived from them of at least about 30 amino acids in length) are homologous, as this term is used herein, if they have an alignment score of more than 5 (in standard deviation units) using the program ALIGN with the mutation data matrix and a gap penalty of 6 or greater. See Dayhoff, M. O., in  Atlas of Protein Sequence and Structure , pp. 101-110 (Volume 5, National Biomedical Research Foundation (1972)) and Supplement 2 to this volume, pp. 1-10. The two sequences or parts thereof are more preferably homologous if their amino acids are greater than or equal to 50% identical when optimally aligned using the ALIGN program. It should be appreciated that there can be differing regions of homology within two orthologous sequences. For example, the functional sites of mouse and human orthologues may have a higher degree of homology than non-functional regions. 
     The term “corresponds to” is used herein to mean that a polynucleotide sequence is homologous (i.e., is identical, not strictly evolutionarily related) to all or a portion of a reference polynucleotide sequence, or that a polypeptide sequence is identical to a reference polypeptide sequence. 
     In contradistinction, the term “complementary to” is used herein to mean that the complementary sequence is homologous to all or a portion of a reference polynucleotide sequence. For illustration, the nucleotide sequence “TATAC” corresponds to a reference sequence “TATAC” and is complementary to a reference sequence “GTATA”. 
     The term “sequence identity” means that two polynucleotide or amino acid sequences are identical (i.e., on a nucleotide-by-nucleotide or residue-by-residue basis) over the comparison window. The term “percentage of sequence identity” is calculated by comparing two optimally aligned sequences over the window of comparison, determining the number of positions at which the identical nucleic acid base (e.g., A, T, C, G, U, or I) or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the comparison window (i.e., the window size), and multiplying the result by 100 to yield the percentage of sequence identity. The terms “substantial identity” as used herein denotes a characteristic of a polynucleotide or amino acid sequence, wherein the polynucleotide or amino acid comprises a sequence that has at least 85 percent sequence identity, preferably at least 90 to 95 percent sequence identity, more preferably at least 99 percent sequence identity, as compared to a reference sequence over a comparison window of at least 18 nucleotide (6 amino acid) positions, frequently over a window of at least 24-48 nucleotide (8-16 amino acid) positions, wherein the percentage of sequence identity is calculated by comparing the reference sequence to the sequence which may include deletions or additions which total 20 percent or less of the reference sequence over the comparison window. The reference sequence may be a subset of a larger sequence. 
     As used herein, the twenty conventional amino acids and their abbreviations follow conventional usage. See  Immunology—A Synthesis  (2 nd  Edition, E. S. Golub and D. R. Gren, Eds., Sinauer Associates, Sunderland, Mass. (1991)), which is incorporated herein by reference. Stereoisomers (e.g., D-amino acids) of the twenty conventional amino acids, unnatural amino acids such as α-,α-disubstituted amino acids, N-alkyl amino acids, lactic acid, and other unconventional amino acids may also be suitable components for polypeptides of the present invention. Examples of unconventional amino acids include: 4-hydroxyproline, γ-carboxyglutamate, ε-N,N,N-trimethyllysine, ε-N-acetyllysine, O-phosphoserine, N-acetylserine, N-formylmethionine, 3-methylhistidine, 5-hydroxylysine, σ-N-methylarginine, and other similar amino acids and imino acids (e.g., 4-hydroxyproline). In the polypeptide notation used herein, the left-hand direction is the amino terminal direction and the right-hand direction is the carboxy-terminal direction, in accordance with standard usage and convention. 
     Similarly, unless specified otherwise, the left-hand end of single-stranded polynucleotide sequences is the 5′ end; the left-hand direction of double-stranded polynucleotide sequences is referred to as the 5′ direction. The direction of 5′ to 3′ addition of nascent RNA transcripts is referred to as the transcription direction; sequence regions on the DNA strand having the same sequence as the RNA and which are 5′ to the 5′ end of the RNA transcript are referred to as “upstream sequences”; sequence regions on the DNA strand having the same sequence as the RNA and which are 3′ to the 3′ end of the RNA transcript are referred to as “downstream sequences”. 
     As applied to polypeptides, the term “substantial identity” means that two peptide sequences, when optimally aligned, such as by the programs GAP or BESTFIT using default gap weights, share at least 80 percent sequence identity, preferably at least 90 percent sequence identity, more preferably at least 95 percent sequence identity, and most preferably at least 99 percent sequence identity. Preferably, residue positions that are not identical differ by conservative amino acid substitutions. Conservative amino acid substitutions refer to the interchangeability of residues having similar side chains. For example, a group of amino acids having aliphatic side chains is glycine, alanine, valine, leucine, and isoleucine; a group of amino acids having aliphatic-hydroxyl side chains is serine and threonine; a group of amino acids having amide-containing side chains is asparagine and glutamine; a group of amino acids having aromatic side chains is phenylalanine, tyrosine, and tryptophan; a group of amino acids having basic side chains is lysine, arginine, and histidine; and a group of amino acids having sulfur-containing side chains is cysteine and methionine. Preferred conservative amino acids substitution groups are: valine-leucine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, glutamic-aspartic, and asparagine-glutamine. 
     As discussed herein, minor variations in the amino acid sequences of antibodies or immunoglobulin molecules are contemplated as being encompassed by the present invention, providing that the variations in the amino acid sequence maintain at least 75%, more preferably at least 80%, 90%, 95%, and most preferably 99% sequence identity to the antibodies or immunoglobulin molecules described herein. In particular, conservative amino acid replacements are contemplated. Conservative replacements are those that take place within a family of amino acids that have related side chains. Genetically encoded amino acids are generally divided into families: (1) acidic=aspartate, glutamate; (2) basic=lysine, arginine, histidine; (3) non-polar=alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and (4) uncharged polar=glycine, asparagine, glutamine, cysteine, serine, threonine, tyrosine. More preferred families are: serine and threonine are an aliphatic-hydroxy family; asparagine and glutamine are an amide-containing family; alanine, valine, leucine and isoleucine are an aliphatic family; and phenylalanine, tryptophan, and tyrosine are an aromatic family. For example, it is reasonable to expect that an isolated replacement of a leucine with an isoleucine or valine, an aspartate with a glutamate, a threonine with a serine, or a similar replacement of an amino acid with a structurally related amino acid will not have a major effect on the binding function or properties of the resulting molecule, especially if the replacement does not involve an amino acid within a framework site. Whether an amino acid change results in a functional peptide can readily be determined by assaying the specific activity of the polypeptide derivative. Assays are described in detail herein. Fragments or analogs of antibodies or immunoglobulin molecules can be readily prepared by those of ordinary skill in the art. Preferred amino- and carboxy-termini of fragments or analogs occur near boundaries of functional domains. Structural and functional domains can be identified by comparison of the nucleotide and/or amino acid sequence data to public or proprietary sequence databases. Preferably, computerized comparison methods are used to identify sequence motifs or predicted protein conformation domains that occur in other proteins of known structure and/or function. Methods to identify protein sequences that fold into a known three-dimensional structure are known. Bowie et al.  Science  253:164 (1991). Thus, the foregoing examples demonstrate that those of skill in the art can recognize sequence motifs and structural conformations that may be used to define structural and functional domains in accordance with the antibodies described herein. 
     Glutaminyl and asparaginyl residues are frequently deamidated to the corresponding glutamyl and aspartyl residues, respectively. These residues are deamidated under neutral or basic conditions. The deamidated form of these residues falls within the scope of this invention. 
     In general, cysteine residues in proteins are either engaged in cysteine-cysteine disulfide bonds or sterically protected from the disulfide bond formation when they are a part of folded protein region. Disulfide bond formation in proteins is a complex process, which is determined by the redox potential of the environment and specialized thiol-disulfide exchanging enzymes (Creighton, Methods Enzymol. 107, 305-329, 1984; Houee-Levin, Methods Enzymol. 353, 35-44,2002). When a cysteine residue does not have a pair in protein structure and is not sterically protected by folding, it can form a disulfide bond with a free cysteine from solution in a process known as disulfide shuffling. In another process known as disulfide scrambling, free cysteines may also interfere with naturally occurring disulfide bonds (such as those present in antibody structures) and lead to low binding, low biological activity and/or low stability. 
     Preferred amino acid substitutions are those which: (1) reduce susceptibility to proteolysis, (2) reduce susceptibility to oxidation, (3) alter binding affinity for forming protein complexes, (4) alter binding affinities, and (4) confer or modify other physicochemical or functional properties of such analogs. Analogs can include various mutations of a sequence other than the naturally-occurring peptide sequence. For example, single or multiple amino acid substitutions (preferably conservative amino acid substitutions) may be made in the naturally-occurring sequence (preferably in the portion of the polypeptide outside the domain(s) forming intermolecular contacts. A conservative amino acid substitution should not substantially change the structural characteristics of the parent sequence (e.g., a replacement amino acid should not tend to break a helix that occurs in the parent sequence, or disrupt other types of secondary structure that characterizes the parent sequence). Examples of art-recognized polypeptide secondary and tertiary structures are described in  Proteins, Structures and Molecular Principles  (Creighton, Ed., W.H. Freeman and Company, New York (1984));  Introduction to Protein Structure  (C. Branden and J. Tooze, eds., Garland Publishing, New York, N.Y. (1991)); and Thornton et al.  Nature  354:105 (1991), which are each incorporated herein by reference. 
     Additionally, such methods may be used to make amino acid substitutions or deletions of one or more variable region cysteine residues participating in an intrachain disulfide bond to generate antibody molecules lacking one or more intrachain disulfide bonds. 
     The term “CDR region” or “CDR” is intended to indicate the hypervariable regions of the heavy and light chains of an antibody which confer antigen-binding specificity to the antibody. CDRs may be defined according to the Kabat system (Kabat, E. A. et al. (1991) Sequences of Proteins of Immunological Interest, 5th Edition. US Department of Health and Human Services, Public Service, NIH, Washington), and later editions. An antibody typically contains 3 heavy chain CDRs and 3 light chain CDRs. The term CDR or CDRs is used here in order to indicate, according to the case, one of these regions or several, or even the whole, of these regions which contain the majority of the amino acid residues responsible for the binding by affinity of the antibody for the antigen or the epitope which it recognises. 
     The third CDR of the heavy chain (HCDR3) has a greater size variability (greater diversity essentially due to the mechanisms of arrangement of the genes which give rise to it). It may be as short as 2 amino acids although the longest size known is 26. CDR length may also vary according to the length that can be accommodated by the particular underlying framework. Functionally, HCDR3 plays a role in part in the determination of the specificity of the antibody (Segal et al., PNAS, 71:4298-4302, 1974, Amit et al., Science, 233:747-753, 1986, Chothia et al., J. Mol. Biol., 196:901-917, 1987, Chothia et al., Nature, 342:877-883, 1989, Caton et al., J. Immunol., 144:1965-1968, 1990, Sharon et al., PNAS, 87:4814-4817, 1990, Sharon et al., J. Immunol., 144:4863-4869, 1990, Kabat et al., J. Immunol., 147:1709-1719, 1991). 
     The term a “set of CDRs” referred to herein comprises CDR1, CDR2 and CDR3. Thus, a set of HCDRs refers to HCDR1, HCDR2 and HCDR3, and a set of LCDRs refers to LCDR1, LCDR2 and LCDR3. 
     Variants of the VH and VL domains and CDRs of the present invention, including those for which amino acid sequences are set out herein, and which can be employed in targeting agents and antibodies for KDR can be obtained by means of methods of sequence alteration or mutation and screening for antigen targeting with desired characteristics. Examples of desired characteristics include but are not limited to: increased binding affinity for antigen relative to known antibodies which are specific for the antigen; increased neutralisation of an antigen activity relative to known antibodies which are specific for the antigen if the activity is known; specified competitive ability with a known antibody or ligand to the antigen at a specific molar ratio; ability to immunoprecipitate ligand-receptor complex; ability to bind to a specified epitope; linear epitope, e.g. peptide sequence identified using peptide-binding scan, e.g. using peptides screened in linear and/or constrained conformation; conformational epitope, formed by non-continuous residues; ability to modulate a new biological activity of KDR, or downstream molecule; ability to bind and/or neutralise KDR and/or for any other desired property. 
     The techniques required to make substitutions within amino acid sequences of CDRs, antibody VH or VL domains and antigen binding sites are available in the art. Variants of antibody molecules disclosed herein may be produced and used in the present invention. Following the lead of computational chemistry in applying multivariate data analysis techniques to the structure/property-activity relationships (Wold, et al. Multivariate data analysis in chemistry. Chemometrics—Mathematics and Statistics in Chemistry (Ed.: B. Kowalski), D. Reidel Publishing Company, Dordrecht, Holland, 1984) quantitative activity-property relationships of antibodies can be derived using well-known mathematical techniques, such as statistical regression, pattern recognition and classification (Norman et al. Applied Regression Analysis. Wiley-Interscience; 3rd edition (April 1998); Kandel, Abraham &amp; Backer, Eric. Computer-Assisted Reasoning in Cluster Analysis. Prentice Hall PTR, (May 11, 1995); Krzanowski, Wojtek. Principles of Multivariate Analysis: A User&#39;s Perspective (Oxford Statistical Science Series, No 22 (Paper)). Oxford University Press; (December 2000); Witten, Ian H. &amp; Frank, Eibe. Data Mining: Practical Machine Learning Tools and Techniques with Java Implementations. Morgan Kaufmann; (Oct. 11, 1999); Denison David G. T. (Editor), Christopher C. Holmes, Bani K. Mallick, Adrian F. M. Smith. Bayesian Methods for Nonlinear Classification and Regression (Wiley Series in Probability and Statistics). John Wiley &amp; Sons; (July 2002); Ghose, Arup K. &amp; Viswanadhan, Vellarkad N. Combinatorial Library Design and Evaluation Principles, Software, Tools, and Applications in Drug Discovery). In some cases the properties of antibodies can be derived from empirical and theoretical models (for example, analysis of likely contact residues or calculated physicochemical property) of antibody sequence, functional and three-dimensional structures and these properties can be considered singly and in combination. 
     An antibody antigen-binding site composed of a VH domain and a VL domain is typically formed by six loops of polypeptide: three from the light chain variable domain (VL) and three from the heavy chain variable domain (VH). Analysis of antibodies of known atomic structure has elucidated relationships between the sequence and three-dimensional structure of antibody combining sites. These relationships imply that, except for the third region (loop) in VH domains, binding site loops have one of a small number of main-chain conformations: canonical structures. The canonical structure formed in a particular loop has been shown to be determined by its size and the presence of certain residues at key sites in both the loop and in framework regions. 
     This study of sequence-structure relationship can be used for prediction of those residues in an antibody of known sequence, but of an unknown three-dimensional structure, which are important in maintaining the three-dimensional structure of its CDR loops and hence maintain binding specificity. These predictions can be backed up by comparison of the predictions to the output from lead optimisation experiments. In a structural approach, a model can be created of the antibody molecule using any freely available or commercial package, such as WAM. A protein visualisation and analysis software package, such as Insight II (Accelrys, Inc.) or Deep View may then be used to evaluate possible substitutions at each position in the CDR. This information may then be used to make substitutions likely to have a minimal or beneficial effect on activity or confer other desirable properties. 
     The term “polypeptide fragment” as used herein refers to a polypeptide that has an amino-terminal and/or carboxy-terminal deletion, but where the remaining amino acid sequence is identical to the corresponding positions in the naturally-occurring sequence deduced, for example, from a full-length cDNA sequence. Fragments typically are at least 5, 6, 8 or 10 amino acids long, preferably at least 14 amino acids long, more preferably at least 20 amino acids long, usually at least 50 amino acids long, and even more preferably at least 70 amino acids long. The term “analog” as used herein refers to polypeptides which are comprised of a segment of at least 25 amino acids that has substantial identity to a portion of a deduced amino acid sequence and which has at least one of the following properties: (1) specific binding to KDR, under suitable binding conditions, (2) ability to block appropriate VEGF/KDR binding, or (3) ability to inhibit KDR receptor tyrosine kinase activity. Typically, polypeptide analogs comprise a conservative amino acid substitution (or addition or deletion) with respect to the naturally-occurring sequence. Analogs typically are at least 20 amino acids long, preferably at least 50 amino acids long or longer, and can often be as long as a full-length naturally-occurring polypeptide. 
     Peptide analogs are commonly used in the pharmaceutical industry as non-peptide drugs with properties analogous to those of the template peptide. These types of non-peptide compound are termed “peptide mimetics” or “peptidomimetics” (Fauchere,  J. Adv. Drug Res.  15:29 (1986); Veber and Freidinger  TINS  p. 392 (1985); and Evans et al.  J. Med. Chem.  30:1229 (1987), which are incorporated herein by reference). Such compounds are often developed with the aid of computerized molecular modeling. Peptide mimetics that are structurally similar to therapeutically useful peptides may be used to produce an equivalent therapeutic or prophylactic effect. Generally, peptidomimetics are structurally similar to a paradigm polypeptide (i.e., a polypeptide that has a biochemical property or pharmacological activity), such as human antibody, but have one or more peptide linkages optionally replaced by a linkage selected from the group consisting of: —CH 2 NH—, —CH 2 S—, —CH 2 —CH 2 —, —CH═CH—(cis and trans), —COCH 2 —, —CH(OH)CH 2 —, and —CH 2 SO—, by methods well known in the art. Systematic substitution of one or more amino acids of a consensus sequence with a D-amino acid of the same type (e.g., D-lysine in place of L-lysine) may be used to generate more stable peptides. In addition, constrained peptides comprising a consensus sequence or a substantially identical consensus sequence variation may be generated by methods known in the art (Rizo and Gierasch  Ann. Rev. Biochem.  61:387 (1992), incorporated herein by reference); for example, by adding internal cysteine residues capable of forming intramolecular disulfide bridges which cyclize the peptide. 
     An antibody may be oligoclonal, a polyclonal antibody, a monoclonal antibody, a chimeric antibody, a CDR-grafted antibody, a multi-specific antibody, a bi-specific antibody, a catalytic antibody, a chimeric antibody, a humanized antibody, a fully human antibody, an anti-idiotypic antibody and antibodies that can be labeled in soluble or bound form as well as fragments, variants or derivatives thereof, either alone or in combination with other amino acid sequences provided by known techniques. An antibody may be from any species. 
     As used herein, the terms “antibody” and “antibodies” (immunoglobulins) encompass monoclonal antibodies (including full-length monoclonal antibodies), polyclonal antibodies, camelised antibodies and chimeric antibodies. As used herein, the term “antibody” or “antibodies” refers to a polypeptide or group of polypeptides that are comprised of at least one binding domain that is formed from the folding of polypeptide chains having three-dimensional binding spaces with internal surface shapes and charge distributions complementary to the features of an antigenic determinant of an antigen. chain. Native antibodies are usually heterotetrameric glycoproteins of about 150,000 daltons, composed of two identical light (L) chains and two identical heavy (H) chains. Each light chain is linked to a heavy chain by one covalent disulfide bond, while the number of disulfide linkages varies between the heavy chains of different immunoglobulin isotypes. Each heavy and light chain also has regularly spaced intrachain disulfide bridges. Each heavy chain has at one end a variable domain (VH) followed by a number of constant domains. Each light chain has a variable domain at one end (VL) and a constant domain at its other end; the constant domain of the light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain. Light chains are classified as either lambda chains or kappa chains based on the amino acid sequence of the light chain constant region. The variable domain of a kappa light chain may also be denoted herein as VK. The term “variable region” may also be used to describe the variable domain of a heavy chain or light chain. Particular amino acid residues are believed to form an interface between the light and heavy chain variable domains. The variable regions of each light/heavy chain pair form an antibody binding site. Such antibodies may be derived from any mammal, including, but not limited to, humans, monkeys, pigs, horses, rabbits, dogs, cats, mice, etc. 
     The term “antibody” or “antibodies” includes binding fragments of the antibodies of the invention, exemplary fragments include single-chain Fvs (scFv), single-chain antibodies, single domain antibodies, domain antibodies, Fv fragments, Fab fragments, F(ab′) fragments, F(ab′)2 fragments, antibody fragments that exhibit the desired biological activity, disulfide-stabilised variable region (dsFv), dimeric variable region (Diabody), anti-idiotypic (anti-Id) antibodies (including, e.g., anti-Id antibodies to antibodies of the invention), intrabodies, linear antibodies, single-chain antibody molecules and multispecific antibodies formed from antibody fragments and epitope-binding fragments of any of the above. In particular, antibodies include immunoglobulin molecules and immunologically active fragments of immunoglobulin molecules, i.e., molecules that contain an antigen-binding site. Immunoglobulin molecules can be of any type (e.g., IgG, IgE, IgM, IgD, IgA and IgY), class (e.g., IgG1, IgG2, IgG3, IgG4, IgA1 and IgA2) or subclass. 
     Digestion of antibodies with the enzyme, papain, results in two identical antigen-binding fragments, known also as “Fab” fragments, and a “Fc” fragment, having no antigen-binding activity but having the ability to crystallize. Digestion of antibodies with the enzyme, pepsin, results in the a F(ab′) 2  fragment in which the two arms of the antibody molecule remain linked and comprise two-antigen binding sites. The F(ab′) 2  fragment has the ability to crosslink antigen. 
     “Fv” when used herein refers to the minimum fragment of an antibody that retains both antigen-recognition and antigen-binding sites. This region consists of a dimer of one heavy and one light chain variable domain in tight, non-covalent or covalent association. It is in this configuration that the three CDRs of each variable domain interact to define an antigen-binding site on the surface of the VH-VL dimer. Collectively, the six CDRs confer antigen-binding specificity to the antibody. However, even a single variable domain (or half of an Fv comprising only three CDRs specific for an antigen) has the ability to recognize and bind antigen, although at a lower affinity than the entire binding site. 
     “Fab” when used herein refers to a fragment of an antibody that comprises the constant domain of the light chain and the CH1 domain of the heavy chain. 
     “dAb” when used herein refers to a fragment of an antibody that is the smallest functional binding unit of a human antibodies. A “dAb” is a single domain antibody and comprises either the variable domain of an antibody heavy chain (VH domain) or the variable domain of an antibody light chain (VL domain). Each dAb contains three of the six naturally occurring CDRs (Ward et al., Binding activities of a repertoire of single immunoglobulin variable domains secreted from  Escherichia coli. Nature  341, 544-546 (1989); Holt, et al., Domain antibodies: protein for therapy,  Trends Biotechnol.  21, 484-49 (2003)). With molecular weights ranging from 11 to 15 kDa, they are four times smaller than a fragment antigen binding (Fab)2 and half the size of a single chain Fv (scFv) molecule. 
     “Camelid” when used herein refers to antibody molecules are composed of heavy-chain dimers which are devoid of light chains, but nevertheless have an extensive antigen-binding repertoire (Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, Hamers C, Songa E B, Bendahman N, Hamers R (1993) Naturally occurring antibodies devoid of light chains. Nature 363:446-448). 
     The term “diabodies” refers to small antibody fragments with two antigen-binding sites, which fragments comprise a heavy chain variable domain (V H ) connected to a light chain variable domain (V L ) in the same polypeptide chain (V H -V L ). By using a linker that is too short to allow pairing between the two domains on the same chain, the domains are forced to pair with the complementary domains of another chain and create two antigen-binding sites. Diabodies are described more fully in, for example, EP 404,097; WO 93/11161; and Hollinger et al.,  Proc. Natl. Acad. Sci. USA,  90:6444-6448 (1993). 
     It has been shown that fragments of a whole antibody can perform the function of binding antigens. Examples of binding fragments are (Ward, E. S. et al., (1989) Nature 341, 544-546) the Fab fragment consisting of VL, VH, CL and CH1 domains; (McCafferty et al (1990) Nature, 348, 552-554) the Fd fragment consisting of the VH and CH1 domains; (Holt et al (2003) Trends in Biotechnology 21, 484-490) the Fv fragment consisting of the VL and VH domains of a single antibody; (iv) the dAb fragment (Ward, E. S. et al., Nature 341, 544-546 (1989), McCafferty et al (1990) Nature, 348, 552-554, Holt et al (2003) Trends in Biotechnology 21, 484-490], which consists of a VH or a VL domain; (v) isolated CDR regions; (vi) F(ab′)2 fragments, a bivalent fragment comprising two linked Fab fragments (vii) single chain Fv molecules (scFv), wherein a VH domain and a VL domain are linked by a peptide linker which allows the two domains to associate to form an antigen binding site (Bird et al, (1988) Science, 242, 423-426, Huston et al, (1988) PNAS USA, 85, 5879-5883); (viii) bispecific single chain Fv dimers (PCT/US92/09965) and (ix) “diabodies”, multivalent or multispecific fragments constructed by gene fusion (WO94/13804; Holliger, P. (1993) et al, Proc. Natl. Acad. Sci. USA 90 6444-6448,). Fv, scFv or diabody molecules may be stabilised by the incorporation of disulphide bridges linking the VH and VL domains (Reiter, Y. et al, Nature Biotech, 14, 1239-1245, 1996). Minibodies comprising a scFv joined to a CH3 domain may also be made (Hu, S. et al, (1996) Cancer Res., 56, 3055-3061). Other examples of binding fragments are Fab′, which differs from Fab fragments by the addition of a few residues at the carboxyl terminus of the heavy chain CH1 domain, including one or more cysteines from the antibody hinge region, and Fab′-SH, which is a Fab′ fragment in which the cysteine residue(s) of the constant domains bear a free thiol group. 
     The term “variable” refers to the fact that certain portions of the variable domains differ extensively in sequence among antibodies and are responsible for the binding specificity of each particular antibody for its particular antigen. However, the variability is not evenly distributed through the variable domains of antibodies. It is concentrated in segments called Complementarity Determining Regions (CDRs) both in the light chain and the heavy chain variable domains. The more highly conserved portions of the variable domains are called the framework regions (FR). The variable domains of native heavy and light chains each comprise four FR regions, largely adopting a β-sheet configuration, connected by three CDRs, which form loops connecting, and in some cases forming part of, the β-sheet structure. The CDRs in each chain are held together in close proximity by the FR regions and, with the CDRs from the other chain, contribute to the formation of the antigen-binding site of antibodies (see, Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)). The constant domains are generally not involved directly in antigen binding, but may influence antigen binding affinity and may exhibit various effector functions, such as participation of the antibody in ADCC, CDC, and/or apoptosis. 
     The term “hypervariable region” when used herein refers to the amino acid residues of an antibody which are associated with its binding to antigen. The hypervariable regions encompass the amino acid residues of the “complementarity determining regions” or “CDRs” (e.g., residues 24-34 (L1), 50-56 (L2) and 89-97 (L3) of the light chain variable domain and residues 31-35 (H1), 50-65 (H2) and 95-102 (H3) of the heavy chain variable domain; Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991)) and/or those residues from a “hypervariable loop” (e.g., residues 26-32 (L1), 50-52 (L2) and 91-96 (L3) in the light chain variable domain and 26-32 (H1), 53-55 (H2) and 96-101 (H3) in the heavy chain variable domain; Chothia and Lesk, J. Mol. Biol., 196:901-917 (1987)). “Framework” or “FR” residues are those variable domain residues flanking the CDRs. FR residues are present in chimeric, humanized, human, domain antibodies, diabodies, vaccibodies, linear antibodies, and bispecific antibodies. 
     As used herein, targeted binding agent, targeted binding protein, specific binding protein and like terms refer to an antibody, or binding fragment thereof that preferentially binds to a target site. In one embodiment, the targeted binding agent is specific for only one target site. In other embodiments, the targeted binding agent is specific for more than one target site. In one embodiment, the targeted binding agent may be a monoclonal antibody and the target site may be an epitope. 
     “Binding fragments” of an antibody are produced by recombinant DNA techniques, or by enzymatic or chemical cleavage of intact antibodies. Binding fragments include Fab, Fab′, F(ab′) 2 , Fv, dAb and single-chain antibodies. An antibody other than a “bispecific” or “bifunctional” antibody is understood to have each of its binding sites identical. An antibody substantially inhibits adhesion of a receptor to a counter-receptor when an excess of antibody reduces the quantity of receptor bound to counter-receptor by at least about 20%, 40%, 60% or 80%, and more usually greater than about 85% (as measured in an in vitro competitive binding assay). 
     The term “epitope” includes any protein determinant capable of specific binding to an immunoglobulin or T-cell receptor. Epitopic determinants usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and may, but not always, have specific three-dimensional structural characteristics, as well as specific charge characteristics. An antibody is said to specifically bind an antigen when the dissociation constant is ≦1 μM, preferably ≦100 nM and most preferably ≦10 nM. 
     The term “agent” is used herein to denote a chemical compound, a mixture of chemical compounds, a biological macromolecule, or an extract made from biological materials. 
     “Active” or “activity” in regard to an KDR polypeptide refers to a portion of an KDR polypeptide that has a biological or an immunological activity of a native KDR polypeptide. “Biological” when used herein refers to a biological function that results from the activity of the native KDR polypeptide. A preferred KDR biological activity includes, for example, KDR induced cell adhesion and invasion and/or angiogenesis and/or proliferation. 
     “Mammal” when used herein refers to any animal that is considered a mammal Preferably, the mammal is human. 
     “Animal” when used herein encompasses animals considered a mammal Preferably the is animal is human. 
     The term “mAb” refers to monoclonal antibody. 
     “Liposome” when used herein refers to a small vesicle that may be useful for delivery of drugs that may include the KDR polypeptide of the invention or antibodies to such an KDR polypeptide to a mammal 
     “Label” or “labeled” as used herein refers to the addition of a detectable moiety to a polypeptide, for example, a radiolabel, fluorescent label, enzymatic label chemiluminescent labeled or a biotinyl group. Radioisotopes or radionuclides may include  3 H,  14 C,  15 N,  35 S,  90 Y,  99 Tc,  111 In,  125 I,  131 I, fluorescent labels may include rhodamine, lanthanide phosphors or FITC and enzymatic labels may include horseradish peroxidase, β-galactosidase, luciferase, alkaline phosphatase. 
     Additional labels include, by way of illustration and not limitation: enzymes, such as glucose-6-phosphate dehydrogenase (“G6PDH”), alpha-D-galactosidase, glucose oxydase, glucose amylase, carbonic anhydrase, acetylcholinesterase, lysozyme, malate dehydrogenase and peroxidase; dyes; additional fluorescent labels or fluorescers include, such as fluorescein and its derivatives, fluorochrome, GFP (GFP for “Green Fluorescent Protein”), dansyl, umbelliferone, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde, and fluorescamine; fluorophores such as lanthanide cryptates and chelates e.g. Europium etc (Perkin Elmer and Cis Biointernational); chemoluminescent labels or chemiluminescers, such as isoluminol, luminol and the dioxetanes; sensitisers; coenzymes; enzyme substrates; particles, such as latex or carbon particles; metal sol; crystallite; liposomes; cells, etc., which may be further labelled with a dye, catalyst or other detectable group; molecules such as biotin, digoxygenin or 5-bromodeoxyuridine; toxin moieties, such as for example a toxin moiety selected from a group of  Pseudomonas  exotoxin (PE or a cytotoxic fragment or mutant thereof), Diptheria toxin or a cytotoxic fragment or mutant thereof, a botulinum toxin A, B, C, D, E or F, ricin or a cytotoxic fragment thereof e.g. ricin A, abrin or a cytotoxic fragment thereof, saporin or a cytotoxic fragment thereof, pokeweed antiviral toxin or a cytotoxic fragment thereof and bryodin 1 or a cytotoxic fragment thereof. 
     The term “pharmaceutical agent or drug” as used herein refers to a chemical compound or composition capable of inducing a desired therapeutic effect when properly administered to a patient. Other chemistry terms herein are used according to conventional usage in the art, as exemplified by  The McGraw - Hill Dictionary of Chemical Terms  (Parker, S., Ed., McGraw-Hill, San Francisco (1985)), (incorporated herein by reference). 
     As used herein, “substantially pure” means an object species is the predominant species present (i.e., on a molar basis it is more abundant than any other individual species in the composition), and preferably a substantially purified fraction is a composition wherein the object species comprises at least about 50 percent (on a molar basis) of all macromolecular species present. Generally, a substantially pure composition will comprise more than about 80 percent of all macromolecular species present in the composition, more preferably more than about 85%, 90%, 95%, and 99%. Most preferably, the object species is purified to essential homogeneity (contaminant species cannot be detected in the composition by conventional detection methods) wherein the composition consists essentially of a single macromolecular species. 
     The term “patient” includes human and veterinary subjects. 
     “Antibody-dependent cell-mediated cytotoxicity” and “ADCC” refer to a cell-mediated reaction in which non-specific cytotoxic cells that express Ig Fc receptors (FcRs) (e.g. Natural Killer (NK) cells, monocytes, neutrophils, and macrophages) recognise bound antibody on a target cell and subsequently cause lysis of the target cell. The primary cells for mediating ADCC, NK cells, express FcγRIII only, whereas monocytes express FcγRI, FcγRII and FcγRIII. FcRs expression on hematopoietic cells is summarised in Table 3 on page 464 of Ravetch and Kinet,  Annu. Rev. Immunol  9:457-92 (1991). To assess ADCC activity of a molecule of interest, an in vitro ADCC assay, such as that described in U.S. Pat. No. 5,500,362, or 5,821,337 can be performed. Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and Natural Killer (NK) cells. Alternatively, or additionally, ADCC activity of the molecule of interest can be assessed in vivo, e.g., in an animal model such as that disclosed in Clynes et al.  PNAS  ( USA ) 95:652-656 (1988). “Complement dependent cytotoxicity” and “CDC” refer to the mechanism by which antibodies carry out their cell-killing function. It is initiated by the binding of C1q, a constituent of the first component of complement, to the Fc domain of Igs, IgG or IgM, which are in complex with antigen (Hughs-Jones, N.C., and B. Gardner. 1979. Mol. Immunol. 16:697). C1q is a large, structurally complex glycoprotein of ˜410 kDa present in human serum at a concentration of 70 μg/ml (Cooper, N. R. 1985. Adv. Immunol. 37:151). Together with two serine proteases, C1r and C1s, C1q forms the complex C1, the first component of complement. At least two of the N-terminal globular heads of C1q must be bound to the Fc of Igs for C1 activation, hence for initiation of the complement cascade (Cooper, N. R. 1985. Adv. Immunol. 37:151). 
     The term “antibody half-life” as used herein means a pharmacokinetic property of an antibody that is a measure of the mean survival time of antibody molecules following their administration. Antibody half-life can be expressed as the time required to eliminate 50 percent of a known quantity of immunoglobulin from the patient&#39;s body or a specific compartment thereof, for example, as measured in serum or plasma, i.e., circulating half-life, or in other tissues. Half-life may vary from one immunoglobulin or class of immunoglobulin to another. In general, an increase in antibody half-life results in an increase in mean residence time (MRT) in circulation for the antibody administered. 
     The term “isotype” refers to the classification of an antibody&#39;s heavy or light chain constant region. The constant domains of antibodies are not involved in binding to antigen, but exhibit various effector functions. Depending on the amino acid sequence of the heavy chain constant region, a given human antibody or immunoglobulin can be assigned to one of five major classes of immunoglobulins: IgA, IgD, IgE, IgG, and IgM. Several of these classes may be further divided into subclasses (isotypes), e.g., IgG1 (gamma 1), IgG2 (gamma 2), IgG3 (gamma 3), and IgG4 (gamma 4), and IgA1 and IgA2. The heavy chain constant regions that correspond to the different classes of immunoglobulins are called α, δ, ε, γ, and μ, respectively. The structures and three-dimensional configurations of different classes of immunoglobulins are well-known. Of the various human immunoglobulin classes, only human IgG1, IgG2, IgG3, IgG4, and IgM are known to activate complement. Human IgG1 and IgG3 are known to mediate in humans. Human light chain constant regions may be classified into two major classes, kappa and lambda. 
     If desired, the isotype of an antibody that specifically binds KDR can be switched, for example to take advantage of a biological property of a different isotype. For example, in some circumstances it can be desirable in connection with the generation of antibodies as therapeutic antibodies against KDR that the antibodies be capable of fixing complement and participating in complement-dependent cytotoxicity (CDC). There are a number of isotypes of antibodies that are capable of the same, including, without limitation, the following: murine IgM, murine IgG2a, murine IgG2b, murine IgG3, human IgM, human IgA, human IgG1, and human IgG3. In other embodiments it can be desirable in connection with the generation of antibodies as therapeutic antibodies against KDR that the antibodies be capable of binding Fc receptors on effector cells and participating in antibody-dependent cytotoxicity (ADCC). There are a number of isotypes of antibodies that are capable of the same, including, without limitation, the following: murine IgG2a, murine IgG2b, murine IgG3, human IgG1, and human IgG3. It will be appreciated that antibodies that are generated need not initially possess such an isotype but, rather, the antibody as generated can possess any isotype and the antibody can be isotype switched thereafter using conventional techniques that are well known in the art. Such techniques include the use of direct recombinant techniques (see e.g., U.S. Pat. No. 4,816,397), cell-cell fusion techniques (see e.g., U.S. Pat. Nos. 5,916,771 and 6,207,418), among others. 
     By way of example, the anti-KDR antibodies discussed herein are fully human antibodies. If an antibody possessed desired binding to KDR, it could be readily isotype switched to generate a human IgM, human IgG1, or human IgG3 isotype, while still possessing the same variable region (which defines the antibody&#39;s specificity and some of its affinity). Such molecule would then be capable of fixing complement and participating in CDC and/or be capable of binding to Fc receptors on effector cells and participating in ADCC. 
     “Whole blood assays” use unfractionated blood as a source of natural effectors. Blood contains complement in the plasma, together with FcR-expressing cellular effectors, such as polymorphonuclear cells (PMNs) and mononuclear cells (MNCs). Thus, whole blood assays allow simultaneous evaluation of the synergy of both ADCC and CDC effector mechanisms in vitro. 
     A “therapeutically effective” amount as used herein is an amount that provides some improvement or benefit to the subject. Stated in another way, a “therapeutically effective” amount is an amount that provides some alleviation, mitigation, and/or decrease in at least one clinical symptom. Clinical symptoms associated with the disorders that can be treated by the methods of the invention are well-known to those skilled in the art. Further, those skilled in the art will appreciate that the therapeutic effects need not be complete or curative, as long as some benefit is provided to the subject. 
     The term “and/or” as used herein is to be taken as specific disclosure of each of the two specified features or components with or without the other. For example “A and/or B” is to be taken as specific disclosure of each of (i) A, (ii) B and (iii) A and B, just as if each is set out individually herein. 
     Antibody Structure 
     The basic antibody structural unit is known to comprise a tetramer. Each tetramer is composed of two identical pairs of polypeptide chains, each pair having one “light” (about 25 kDa) and one “heavy” chain (about 50-70 kDa). The amino-terminal portion of each chain includes a variable region of about 100 to 110 or more amino acids primarily responsible for antigen recognition. The carboxy-terminal portion of each chain defines a constant region primarily responsible for effector function. Human light chains are classified as kappa and lambda light chains. Heavy chains are classified as mu, delta, gamma, alpha, or epsilon, and define the antibody&#39;s isotype as IgM, IgD, IgA, and IgE, respectively. Within light and heavy chains, the variable and constant regions are joined by a “J” region of about 12 or more amino acids, with the heavy chain also including a “D” region of about 10 more amino acids. See generally,  Fundamental Immunology  Ch. 7 (Paul, W., ed., 2nd ed. Raven Press, N.Y. (1989)) (incorporated by reference in its entirety for all purposes). The variable regions of each light/heavy chain pair form the antibody binding site. 
     Thus, an intact antibody has two binding sites. Except in bifunctional or bispecific antibodies, the two binding sites are the same. 
     The chains all exhibit the same general structure of relatively conserved framework regions (FR) joined by three hyper variable regions, also called CDRs. The CDRs from the two chains of each pair are aligned by the framework regions, enabling binding to a specific epitope. From N-terminal to C-terminal, both light and heavy chains comprise the domains FR1, CDR1, FR2, CDR2, FR3, CDR3 and FR4. The assignment of amino acids to each domain is in accordance with the definitions of Kabat  Sequences of Proteins of Immunological Interest  (National Institutes of Health, Bethesda, Md. (1987 and 1991)), or Chothia &amp; Lesk  J. Mol. Biol.  196:901-917 (1987); Chothia et al.  Nature  342:878-883 (1989). 
     A bispecific or bifunctional antibody is an artificial hybrid antibody having two different heavy/light chain pairs and two different binding sites. Bispecific antibodies can be produced by a variety of methods including fusion of hybridomas or linking of Fab′ fragments. See, e.g., Songsivilai &amp; Lachmann  Clin. Exp. Immunol.  79: 315-321 (1990), Kostelny et al.  J. Immunol.  148:1547-1553 (1992). Bispecific antibodies do not exist in the form of fragments having a single binding site (e.g., Fab, Fab′, and Fv). 
     Typically, a VH domain is paired with a VL domain to provide an antibody antigen-binding site, although a VH or VL domain alone may be used to bind antigen. The VH domain (see Table 12) may be paired with the VL domain (see Table 13), so that an antibody antigen-binding site is formed comprising both the VH and VL domains. 
     Human Antibodies and Humanization of Antibodies 
     Human antibodies avoid some of the problems associated with antibodies that possess murine or rat variable and/or constant regions. The presence of such murine or rat derived proteins can lead to the rapid clearance of the antibodies or can lead to the generation of an immune response against the antibody by a patient. In order to avoid the utilization of murine or rat derived antibodies, fully human antibodies can be generated through the introduction of functional human antibody loci into a rodent, other mammal or animal so that the rodent, other mammal or animal produces fully human antibodies. 
     One method for generating fully human antibodies is through the use of XenoMouse® strains of mice that have been engineered to contain up to but less than 1000 kb-sized germline configured fragments of the human heavy chain locus and kappa light chain locus. See Mendez et al.  Nature Genetics  15:146-156 (1997) and Green and Jakobovits  J. Exp. Med.  188:483-495 (1998). The XenoMouse® strains are available from Amgen, Inc. (Fremont, Calif., U.S.A). 
     Such mice, then, are capable of producing human immunoglobulin molecules and antibodies and are deficient in the production of murine immunoglobulin molecules and antibodies. Technologies utilised for achieving the same are disclosed in U.S. patent application Ser. No. 08/759,620, filed Dec. 3, 1996 and International Patent Application Nos. WO 98/24893, published Jun. 11, 1998 and WO 00/76310, published Dec. 21, 2000, the disclosures of which are hereby incorporated by reference. See also Mendez et al.  Nature Genetics  15:146-156 (1997), the disclosure of which is hereby incorporated by reference. 
     The production of the XenoMouse® strains of mice is further discussed and delineated in U.S. patent application Ser. No. 07/466,008, filed Jan. 12, 1990, U.S. patent application Ser. No. 07/610,515, filed Nov. 8, 1990, U.S. patent application Ser. No. 07/919,297, filed Jul. 24, 1992, U.S. patent application Ser. No. 07/922,649, filed Jul. 30, 1992, U.S. patent application Ser. No. 08/031,801, filed Mar. 15, 1993, U.S. patent application Ser. No. 08/112,848, filed Aug. 27, 1993, U.S. patent application Ser. No. 08/234,145, filed Apr. 28, 1994, U.S. patent application Ser. No. 08/376,279, filed Jan. 20, 1995, U.S. patent application Ser. No. 08/430, 938, filed Apr. 27, 1995, U.S. patent application Ser. No. 08/464,584, filed Jun. 5, 1995, U.S. patent application Ser. No. 08/464,582, filed Jun. 5, 1995, U.S. patent application Ser. No. 08/463,191, filed Jun. 5, 1995, U.S. patent application Ser. No. 08/462,837, filed Jun. 5, 1995, U.S. patent application Ser. No. 08/486,853, filed Jun. 5, 1995, U.S. patent application Ser. No. 08/486,857, filed Jun. 5, 1995, U.S. patent application Ser. No. 08/486,859, filed Jun. 5, 1995, U.S. patent application Ser. No. 08/462,513, filed Jun. 5, 1995, U.S. patent application Ser. No. 08/724,752, filed Oct. 2, 1996, U.S. patent application Ser. No. 08/759,620, filed Dec. 3, 1996, U.S. Publication 2003/0093820, filed Nov. 30, 2001 and U.S. Pat. Nos. 6,162,963, 6,150,584, 6,114,598, 6,075,181, and 5,939,598 and Japanese Patent Nos. 3 068 180 B2, 3 068 506 B2, and 3 068 507 B2. See also European Patent No., EP 0 463 151 B1, grant published Jun. 12, 1996, International Patent Application No., WO 94/02602, published Feb. 3, 1994, International Patent Application No., WO 96/34096, published Oct. 31, 1996, WO 98/24893, published Jun. 11, 1998, WO 00/76310, published Dec. 21, 2000. The disclosures of each of the above-cited patents, applications, and references are hereby incorporated by reference in their entirety. 
     In an alternative approach, others, including GenPharm International, Inc., have utilised a “minilocus” approach. In the minilocus approach, an exogenous Ig locus is mimicked through the inclusion of pieces (individual genes) from the Ig locus. Thus, one or more V H  genes, one or more D H  genes, one or more J H  genes, a mu constant region, and usually a second constant region (preferably a gamma constant region) are formed into a construct for insertion into an animal. This approach is described in U.S. Pat. No. 5,545,807 to Surani et al. and U.S. Pat. Nos. 5,545,806, 5,625,825, 5,625,126, 5,633,425, 5,661,016, 5,770,429, 5,789,650, 5,814,318, 5,877,397, 5,874,299, and 6,255,458 each to Lonberg and Kay, U.S. Pat. Nos. 5,591,669 and 6,023,010 to Krimpenfort and Berns, U.S. Pat. Nos. 5,612,205, 5,721,367, and 5,789,215 to Berns et al., and U.S. Pat. No. 5,643,763 to Choi and Dunn, and GenPharm International U.S. patent application Ser. No. 07/574,748, filed Aug. 29, 1990, U.S. patent application Ser. No. 07/575,962, filed Aug. 31, 1990, U.S. patent application Ser. No. 07/810,279, filed Dec. 17, 1991, U.S. patent application Ser. No. 07/853,408, filed Mar. 18, 1992, U.S. patent application Ser. No. 07/904,068, filed Jun. 23, 1992, U.S. patent application Ser. No. 07/990,860, filed Dec. 16, 1992, U.S. patent application Ser. No. 08/053,131, filed Apr. 26, 1993, U.S. patent application Ser. No. 08/096,762, filed Jul. 22, 1993, U.S. patent application Ser. No. 08/155,301, filed Nov. 18, 1993, U.S. patent application Ser. No. 08/161,739, filed Dec. 3, 1993, U.S. patent application Ser. No. 08/165,699, filed Dec. 10, 1993, U.S. patent application Ser. No. 08/209,741, filed Mar. 9, 1994, the disclosures of which are hereby incorporated by reference. See also European Patent No. 0 546 073 B1, International Patent Application Nos. WO 92/03918, WO 92/22645, WO 92/22647, WO 92/22670, WO 93/12227, WO 94/00569, WO 94/25585, WO 96/14436, WO 97/13852, and WO 98/24884 and U.S. Pat. No. 5,981,175, the disclosures of which are hereby incorporated by reference in their entirety. See further Taylor et al., 1992, Chen et al., 1993, Tuaillon et al., 1993, Choi et al., 1993, Lonberg et al., (1994), Taylor et al., (1994), and Tuaillon et al., (1995), Fishwild et al., (1996), the disclosures of which are hereby incorporated by reference in their entirety. 
     Kirin has also demonstrated the generation of human antibodies from mice in which, through microcell fusion, large pieces of chromosomes, or entire chromosomes, have been introduced. See European Patent Application Nos. 773 288 and 843 961, the disclosures of which are hereby incorporated by reference. Additionally, KM™-mice, which are the result of cross-breeding of Kirin&#39;s Tc mice with Medarex&#39;s minilocus (Humab) mice have been generated. These mice possess the human IgH transchromosome of the Kirin mice and the kappa chain transgene of the Genpharm mice (Ishida et al., Cloning Stem Cells, (2002) 4:91-102). 
     Human antibodies can also be derived by in vitro methods. Suitable examples include but are not limited to phage display (Medimmune, Morphosys, Dyax, Biosite/Medarex, Xoma, Symphogen, Alexion (formerly Proliferon), Affimed) ribosome display (Medimmune), yeast display, and the like. 
     Preparation of Antibodies 
     Antibodies, as described herein, were prepared through the utilization of the XenoMouse® technology, as described below. Such mice are capable of producing human immunoglobulin molecules and antibodies and are deficient in the production of murine immunoglobulin molecules and antibodies. Technologies utilised for achieving the same are disclosed in the patents, applications, and references disclosed in the background section herein. In particular, however, a preferred embodiment of transgenic production of mice and antibodies therefrom is disclosed in U.S. patent application Ser. No. 08/759,620, filed Dec. 3, 1996 and International Patent Application Nos. WO 98/24893, published Jun. 11, 1998 and WO 00/76310, published Dec. 21, 2000, the disclosures of which are hereby incorporated by reference. See also Mendez et al.  Nature Genetics  15:146-156 (1997), the disclosure of which is hereby incorporated by reference. 
     Through the use of such technology, fully human monoclonal antibodies to a variety of antigens have been produced. Essentially, XenoMouse® lines of mice are immunised with an antigen of interest (e.g. KDR), lymphatic cells (such as B-cells) are recovered from the hyperimmunised mice, and the recovered lymphocytes are fused with a myeloid-type cell line to prepare immortal hybridoma cell lines. These hybridoma cell lines are screened and selected to identify hybridoma cell lines that produced antibodies specific to the antigen of interest. Provided herein are methods for the production of multiple hybridoma cell lines that produce antibodies specific to KDR. Further, provided herein are characterisation of the antibodies produced by such cell lines, including nucleotide and amino acid sequence analyses of the heavy and light chains of such antibodies. 
     Alternatively, instead of being fused to myeloma cells to generate hybridomas, B cells can be directly assayed. For example, CD19+ B cells can be isolated from hyperimmune XenoMouse® mice and allowed to proliferate and differentiate into antibody-secreting plasma cells. Antibodies from the cell supernatants are then screened by ELISA for reactivity against the KDR immunogen. The supernatants might also be screened for immunoreactivity against fragments of KDR to further map the different antibodies for binding to domains of functional interest on KDR. The antibodies may also be screened other related human endoglycosidases and against the rat, the mouse, and non-human primate, such as Cynomolgus monkey, orthologues of KDR, the last to determine species cross-reactivity. B cells from wells containing antibodies of interest may be immortalised by various methods including fusion to make hybridomas either from individual or from pooled wells, or by infection with EBV or transfection by known immortalising genes and then plating in suitable medium. Alternatively, single plasma cells secreting antibodies with the desired specificities are then isolated using an KDR-specific hemolytic plaque assay (see for example Babcook et al.,  Proc. Natl. Acad. Sci. USA  93:7843-48 (1996)). Cells targeted for lysis are preferably sheep red blood cells (SRBCs) coated with the KDR antigen. 
     In the presence of a B-cell culture containing plasma cells secreting the immunoglobulin of interest and complement, the formation of a plaque indicates specific KDR-mediated lysis of the sheep red blood cells surrounding the plasma cell of interest. The single antigen-specific plasma cell in the center of the plaque can be isolated and the genetic information that encodes the specificity of the antibody is isolated from the single plasma cell. Using reverse-transcription followed by PCR (RT-PCR), the DNA encoding the heavy and light chain variable regions of the antibody can be cloned. Such cloned DNA can then be further inserted into a suitable expression vector, preferably a vector cassette such as a pcDNA, more preferably such a pcDNA vector containing the constant domains of immunglobulin heavy and light chain. The generated vector can then be transfected into host cells, e.g., HEK293 cells, CHO cells, and cultured in conventional nutrient media modified as appropriate for inducing transcription, selecting transformants, or amplifying the genes encoding the desired sequences. 
     As will be appreciated, antibodies that specifically bind KDR can be expressed in cell lines other than hybridoma cell lines. Sequences encoding particular antibodies can be used to transform a suitable mammalian host cell. Transformation can be by any known method for introducing polynucleotides into a host cell, including, for example packaging the polynucleotide in a virus (or into a viral vector) and transducing a host cell with the virus (or vector) or by transfection procedures known in the art, as exemplified by U.S. Pat. Nos. 4,399,216, 4,912,040, 4,740,461, and 4,959,455 (which patents are hereby incorporated herein by reference). The transformation procedure used depends upon the host to be transformed. Methods for introducing heterologous polynucleotides into mammalian cells are well known in the art and include dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) in liposomes, and direct microinjection of the DNA into nuclei. 
     Mammalian cell lines available as hosts for expression are well known in the art and include many immortalized cell lines available from the American Type Culture Collection (ATCC), including but not limited to Chinese hamster ovary (CHO) cells, HeLa cells, baby hamster kidney (BHK) cells, monkey kidney cells (COS), human hepatocellular carcinoma cells (e.g., Hep G2), human epithelial kidney 293 cells, and a number of other cell lines. Cell lines of particular preference are selected through determining which cell lines have high expression levels and produce antibodies with constitutive KDR binding properties. 
     In the cell-cell fusion technique, a myeloma, CHO cell or other cell line is prepared that possesses a heavy chain with any desired isotype and another myeloma, CHO cell or other cell line is prepared that possesses the light chain. Such cells can, thereafter, be fused and a cell line expressing an intact antibody can be isolated. 
     Accordingly, as antibody candidates are generated that meet desired “structural” attributes as discussed above, they can generally be provided with at least certain of the desired “functional” attributes through isotype switching. 
     Therapeutic Administration and Formulations 
     Embodiments of the invention include sterile pharmaceutical formulations of anti-KDR antibodies that are useful as treatments for diseases. Such formulations would inhibit the binding of a native KDR-specific ligand such as, for example, VEGF, to KDR, thereby effectively treating pathological conditions where, for example, serum or tissue KDR expression is abnormally elevated. Anti-KDR antibodies preferably possess adequate affinity to potently inhibit native KDR-specific ligands such as, for example, VEGF, and preferably have an adequate duration of action to allow for infrequent dosing in humans. A prolonged duration of action will allow for less frequent and more convenient dosing schedules by alternate parenteral routes such as subcutaneous or intramuscular injection. 
     Sterile formulations can be created, for example, by filtration through sterile filtration membranes, prior to or following lyophilization and reconstitution of the antibody. The antibody ordinarily will be stored in lyophilized form or in solution. Therapeutic antibody compositions generally are placed into a container having a sterile access port, for example, an intravenous solution bag or vial having an adapter that allows retrieval of the formulation, such as a stopper pierceable by a hypodermic injection needle. 
     The route of antibody administration is in accord with known methods, e.g., injection or infusion by intravenous, intraperitoneal, intracerebral, intramuscular, intraocular, intraarterial, intrathecal, inhalation or intralesional routes, direct injection to a tumour site, or by sustained release systems as noted below. The antibody is preferably administered continuously by infusion or by bolus injection. 
     An effective amount of antibody to be employed therapeutically will depend, for example, upon the therapeutic objectives, the route of administration, and the condition of the patient. Accordingly, it is preferred that the therapist titer the dosage and modify the route of administration as required to obtain the optimal therapeutic effect. Typically, the clinician will administer antibody until a dosage is reached that achieves the desired effect. The progress of this therapy is easily monitored by conventional assays or by the assays described herein. 
     Antibodies, as described herein, can be prepared in a mixture with a pharmaceutically acceptable carrier. This therapeutic composition can be administered intravenously or through the nose or lung, preferably as a liquid or powder aerosol (lyophilized). The composition may also be administered parenterally or subcutaneously as desired. When administered systemically, the therapeutic composition should be sterile, pyrogen-free and in a parenterally acceptable solution having due regard for pH, isotonicity, and stability. These conditions are known to those skilled in the art. Briefly, dosage formulations of the compounds described herein are prepared for storage or administration by mixing the compound having the desired degree of purity with pharmaceutically acceptable carriers, excipients, or stabilizers. Such materials are non-toxic to the recipients at the dosages and concentrations employed, and include buffers such as TRIS HCl, phosphate, citrate, acetate and other organic acid salts; antioxidants such as ascorbic acid; low molecular weight (less than about ten residues) peptides such as polyarginine, proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidinone; amino acids such as glycine, glutamic acid, aspartic acid, or arginine; monosaccharides, disaccharides, and other carbohydrates including cellulose or its derivatives, glucose, mannose, or dextrins; chelating agents such as EDTA; sugar alcohols such as mannitol or sorbitol; counterions such as sodium and/or nonionic surfactants such as TWEEN, PLURONICS or polyethyleneglycol. 
     Sterile compositions for injection can be formulated according to conventional pharmaceutical practice as described in  Remington: The Science and Practice of Pharmacy  (20 th  ed, Lippincott Williams &amp; Wilkens Publishers (2003)). For example, dissolution or suspension of the active compound in a pharmaceutically acceptable carrier such as water or naturally occurring vegetable oil like sesame, peanut, or cottonseed oil or a synthetic fatty vehicle like ethyl oleate or the like may be desired. Buffers, preservatives, antioxidants and the like can be incorporated according to accepted pharmaceutical practice. 
     Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the polypeptide, which matrices are in the form of shaped articles, films or microcapsules. Examples of sustained-release matrices include polyesters, hydrogels (e.g., poly(2-hydroxyethyl-methacrylate) as described by Langer et al.,  J. Biomed Mater. Res ., (1981) 15:167-277 and Langer,  Chem. Tech ., (1982) 12:98-105, or poly(vinylalcohol)), polylactides (U.S. Pat. No. 3,773,919, EP 58,481), copolymers of L-glutamic acid and gamma ethyl-L-glutamate (Sidman et al.,  Biopolymers , (1983) 22:547-556), non-degradable ethylene-vinyl acetate (Langer et al., supra), degradable lactic acid-glycolic acid copolymers such as the LUPRON Depot™ (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(−)-3-hydroxybutyric acid (EP 133,988). 
     While polymers such as ethylene-vinyl acetate and lactic acid-glycolic acid enable release of molecules for over 100 days, certain hydrogels release proteins for shorter time periods. When encapsulated proteins remain in the body for a long time, they may denature or aggregate as a result of exposure to moisture at 37° C., resulting in a loss of biological activity and possible changes in immunogenicity. Rational strategies can be devised for protein stabilization depending on the mechanism involved. For example, if the aggregation mechanism is discovered to be intermolecular S—S bond formation through disulfide interchange, stabilization may be achieved by modifying sulfhydryl residues, lyophilizing from acidic solutions, controlling moisture content, using appropriate additives, and developing specific polymer matrix compositions. 
     Sustained-released compositions also include preparations of crystals of the antibody suspended in suitable formulations capable of maintaining crystals in suspension. These preparations when injected subcutaneously or intraperitonealy can produce a sustained release effect. Other compositions also include liposomally entrapped antibodies. Liposomes containing such antibodies are prepared by methods known per se: U.S. Pat. No. DE 3,218,121; Epstein et al.,  Proc. Natl. Acad. Sci. USA , (1985) 82:3688-3692; Hwang et al.,  Proc. Natl. Acad. Sci. USA , (1980) 77:4030-4034; EP 52,322; EP 36,676; EP 88,046; EP 143,949; 142,641; Japanese patent application 83-118008; U.S. Pat. Nos. 4,485,045 and 4,544,545; and EP 102,324. 
     The dosage of the antibody formulation for a given patient will be determined by the attending physician taking into consideration various factors known to modify the action of drugs including severity and type of disease, body weight, sex, diet, time and route of administration, other medications and other relevant clinical factors. Therapeutically effective dosages may be determined by either in vitro or in vivo methods. 
     An effective amount of the antibodies, described herein, to be employed therapeutically will depend, for example, upon the therapeutic objectives, the route of administration, and the condition of the patient. Accordingly, it is preferred for the therapist to titer the dosage and modify the route of administration as required to obtain the optimal therapeutic effect. A typical daily dosage might range from about 0.0001 mg/kg, 0.001 mg/kg, 0.01 mg/kg, 0.1 mg/kg, 1 mg/kg, 10 mg/kg to up to 100 mg/kg, 1000 mg/kg, 10000 mg/kg or more, of the patient&#39;s body weight depending on the factors mentioned above. The dosage may be between 0.0001 mg/kg and 20 mg/kg, 0.0001 mg/kg and 10 mg/kg, 0.0001 mg/kg and 5 mg/kg, 0.0001 and 2 mg/kg, 0.0001 and 1 mg/kg, 0.0001 mg/kg and 0.75 mg/kg, 0.0001 mg/kg and 0.5 mg/kg, 0.0001 mg/kg to 0.25 mg/kg, 0.0001 to 0.15 mg/kg, 0.0001 to 0.10 mg/kg, 0.001 to 0.5 mg/kg, 0.01 to 0.25 mg/kg or 0.01 to 0.10 mg/kg of the patient&#39;s body weight depending on the factors mentioned above. Typically, the clinician will administer the therapeutic antibody until a dosage is reached that achieves the desired effect. The progress of this therapy is easily monitored by conventional assays or as described herein. 
     Doses of antibodies of the invention may be repeated and the administrations may be separated by at least 1 day, 2 days, 3 days, 5 days, 10 days, 15 days, 30 days, 45 days, 2 months, 75 days, 3 months, or at least 6 months. 
     It will be appreciated that administration of therapeutic entities in accordance with the compositions and methods herein will be administered with suitable carriers, excipients, and other agents that are incorporated into formulations to provide improved transfer, delivery, tolerance, and the like. These formulations include, for example, powders, pastes, ointments, jellies, waxes, oils, lipids, lipid (cationic or anionic) containing vesicles (such as Lipofectin™) DNA conjugates, anhydrous absorption pastes, oil-in-water and water-in-oil emulsions, emulsions carbowax (polyethylene glycols of various molecular weights), semi-solid gels, and semi-solid mixtures containing carbowax. Any of the foregoing mixtures may be appropriate in treatments and therapies in accordance with the present invention, provided that the active ingredient in the formulation is not inactivated by the formulation and the formulation is physiologically compatible and tolerable with the route of administration. See also Baldrick P. “Pharmaceutical excipient development: the need for preclinical guidance.”  Regul. Toxicol. Pharmacol.  32(2):210-8 (2000), Wang W. “Lyophilization and development of solid protein pharmaceuticals.”  Int. J. Pharm.  203(1-2):1-60 (2000), Charman W N “Lipids, lipophilic drugs, and oral drug delivery-some emerging concepts.”  J Pharm Sci.  89(8):967-78 (2000), Powell et al. “Compendium of excipients for parenteral formulations”  PDA J Pharm Sci Technol.  52:238-311 (1998) and the citations therein for additional information related to formulations, excipients and carriers well known to pharmaceutical chemists. 
     Design and Generation of Other Therapeutics 
     In accordance with the present invention and based on the activity of the antibodies that are produced and characterized herein with respect to KDR, the design of other therapeutic modalities beyond antibody moieties is facilitated. Such modalities include, without limitation, advanced antibody therapeutics, such as bispecific antibodies, immunotoxins, and radiolabeled therapeutics, single domain antibodies, antibody fragments, such as a Fab, Fab′, F(ab′) 2 , Fv or dAb, generation of peptide therapeutics, KDR binding domains in novel scaffolds, gene therapies, particularly intrabodies, antisense therapeutics, and small molecules. 
     An antigen binding site may be provided by means of arrangement of CDRs on non-antibody protein scaffolds, such as fibronectin or cytochrome B etc. (Haan &amp; Maggos (2004) BioCentury, 12(5): A1-A6; Koide et al. (1998) Journal of Molecular Biology, 284: 1141-1151; Nygren et al. (1997) Current Opinion in Structural Biology, 7: 463-469) or by randomising or mutating amino acid residues of a loop within a protein scaffold to confer binding specificity for a desired target. Scaffolds for engineering novel binding sites in proteins have been reviewed in detail by Nygren et al. (Nygren et al. (1997) Current Opinion in Structural Biology, 7: 463-469). Protein scaffolds for antibody mimics are disclosed in WO/0034784, which is herein incorporated by reference in its entirety, in which the inventors describe proteins (antibody mimics) that include a fibronectin type III domain having at least one randomised loop. A suitable scaffold into which to graft one or more CDRs, e.g. a set of HCDRs, may be provided by any domain member of the immunoglobulin gene superfamily. The scaffold may be a human or non-human protein. An advantage of a non-antibody protein scaffold is that it may provide an antigen-binding site in a scaffold molecule that is smaller and/or easier to manufacture than at least some antibody molecules. Small size of a binding member may confer useful physiological properties, such as an ability to enter cells, penetrate deep into tissues or reach targets within other structures, or to bind within protein cavities of the target antigen. Use of antigen binding sites in non-antibody protein scaffolds is reviewed in Wess, 2004 (Wess, L. In: BioCentury, The Bernstein Report on BioBusiness, 12(42), A1-A7, 2004). Typical are proteins having a stable backbone and one or more variable loops, in which the amino acid sequence of the loop or loops specifically or randomly mutated to create an antigen-binding site that binds the target antigen. Such proteins include the IgG-binding domains of protein A from  S. aureus , transferrin, albumin, tetranectin, fibronectin (e.g. 10th fibronectin type III domain), lipocalins as well as gamma-crystalline and other Affilin™ scaffolds (Scil Proteins). Examples of other approaches include synthetic “Microbodies” based on cyclotides—small proteins having intra-molecular disulphide bonds, Microproteins (Versabodies™, Amunix) and ankyrin repeat proteins (DARPins, Molecular Partners). 
     In addition to antibody sequences and/or an antigen-binding site, a targeted binding agent according to the present invention may comprise other amino acids, e.g. forming a peptide or polypeptide, such as a folded domain, or to impart to the molecule another functional characteristic in addition to ability to bind antigen. Targeted binding agents of the invention may carry a detectable label, or may be conjugated to a toxin or a targeting moiety or enzyme (e.g. via a peptidyl bond or linker). For example, a targeted binding agent may comprise a catalytic site (e.g. in an enzyme domain) as well as an antigen binding site, wherein the antigen binding site binds to the antigen and thus targets the catalytic site to the antigen. The catalytic site may inhibit biological function of the antigen, e.g. by cleavage. 
     In connection with the generation of advanced antibody therapeutics, where complement fixation is a desirable attribute, it may be possible to sidestep the dependence on complement for cell killing through the use of bispecific antibodies, immunotoxins, or radiolabels, for example. 
     For example, bispecific antibodies can be generated that comprise (i) two antibodies one with a specificity to KDR and another to a second molecule that are conjugated together, (ii) a single antibody that has one chain specific to KDR and a second chain specific to a second molecule, or (iii) a single chain antibody that has specificity to KDR and the other molecule. Such bispecific antibodies can be generated using techniques that are well known; for example, in connection with (i) and (ii) see e.g., Fanger et al.  Immunol Methods  4:72-81 (1994) and Wright and Harris, supra. and in connection with (iii) see e.g., Traunecker et al.  Int. J. Cancer  ( Suppl .) 7:51-52 (1992). In each case, the second specificity can be made to the heavy chain activation receptors, including, without limitation, CD16 or CD64 (see e.g., Deo et al.  Immunol. Today  18:127 (1997)) or CD89 (see e.g., Valerius et al.  Blood  90:4485-4492 (1997)). 
     Antibodies can also be modified to act as immunotoxins, utilizing techniques that are well known in the art. See e.g., Vitetta  Immunol Today  14:252 (1993). See also U.S. Pat. No. 5,194,594. In connection with the preparation of radiolabeled antibodies, such modified antibodies can also be readily prepared utilizing techniques that are well known in the art. See e.g., Junghans et al. in  Cancer Chemotherapy and Biotherapy  655-686 (2d edition, Chafner and Longo, eds., Lippincott Raven (1996)). See also U.S. Pat. Nos. 4,681,581, 4,735,210, 5,101,827, 5,102,990 (RE 35,500), 5,648,471, and 5,697,902. Each immunotoxin or radiolabeled molecule would be likely to kill cells expressing the desired multimeric enzyme subunit oligomerisation domain. 
     When an antibody is linked to an agent (e.g., radioisotope, pharmaceutical composition, or a toxin), it is contemplated that the agent possess a pharmaceutical property selected from the group of antimitotic, alkylating, antimetabolite, antiangiogenic, apoptotic, alkaloid, COX-2, and antibiotic agents and combinations thereof. The drug can be selected from the group of nitrogen mustards, ethylenimine derivatives, alkyl sulfonates, nitrosoureas, triazenes, folic acid analogs, anthracyclines, taxanes, COX-2 inhibitors, pyrimidine analogs, purine analogs, antimetabolites, antibiotics, enzymes, epipodophyllotoxins, platinum coordination complexes, vinca alkaloids, substituted ureas, methyl hydrazine derivatives, adrenocortical suppressants, antagonists, endostatin, taxols, camptothecins, oxaliplatin, doxorubicins and their analogs, and a combination thereof. 
     Examples of toxins further include gelonin,  Pseudomonas  exotoxin (PE), PE40, PE38, diphtheria toxin, ricin, abrin, alpha toxin, saporin, ribonuclease (RNase), DNase I, Staphylococcal enterotoxin-A, pokeweed antiviral protein, gelonin,  Pseudomonas  endotoxin, members of the enediyne family of molecules, such as calicheamicin and esperamicin, as well as derivatives, combinations and modifications thereof. Chemical toxins can also be taken from the group consisting of duocarmycin (see, e.g., U.S. Pat. Nos. 5,703,080 and 4,923,990), methotrexate, doxorubicin, melphalan, chlorambucil, ARA-C, vindesine, mitomycin C, cis-platinum, etoposide, bleomycin and 5-fluorouracil. Examples of chemotherapeutic agents also include Adriamycin, Doxorubicin, 5-Fluorouracil, Cytosine arabinoside (Ara-C), Cyclophosphamide, Thiotepa, Taxotere (docetaxel), Busulfan, Cytoxin, Taxol, Methotrexate, Cisplatin, Melphalan, Vinblastine, Bleomycin, Etoposide, Ifosfamide, Mitomycin C, Mitoxantrone, Vincreistine, Vinorelbine, Carboplatin, Teniposide, Daunomycin, Caminomycin, Aminopterin, Dactinomycin, Mitomycins, Esperamicins (see, U.S. Pat. No. 4,675,187), Melphalan, and other related nitrogen mustards. Suitable toxins and chemotherapeutic agents are described in Remington&#39;s Pharmaceutical Sciences, 19th Ed. (Mack Publishing Co. 1995), and in Goodman And Gilman&#39;s The Pharmacological Basis of Therapeutics, 7th Ed. (MacMillan Publishing Co. 1985). Other suitable toxins and/or chemotherapeutic agents are known to those of skill in the art. 
     Examples of radioisotopes include gamma-emitters, positron-emitters, and x-ray emitters that can be used for localisation and/or therapy, and beta-emitters and alpha-emitters that can be used for therapy. The radioisotopes described previously as useful for diagnostics, prognostics and staging are also useful for therapeutics. 
     Non-limiting examples of anti-cancer or anti-leukemia agents include anthracyclines such as doxorubicin (adriamycin), daunorubicin (daunomycin), idarubicin, detorubicin, caminomycin, epirubicin, esorubicin, and morpholino and substituted derivatives, combinations and modifications thereof. Exemplary pharmaceutical agents include cis-platinum, taxol, calicheamicin, vincristine, cytarabine (Ara-C), cyclophosphamide, prednisone, daunorubicin, idarubicin, fludarabine, chlorambucil, interferon alpha, hydroxyurea, temozolomide, thalidomide, and bleomycin, and derivatives, combinations and modifications thereof. Preferably, the anti-cancer or anti-leukemia is doxorubicin, morpholinodoxorubicin, or morpholinodaunorubicin. 
     The antibodies of the invention also encompass antibodies that have half-lives (e.g., serum half-lives) in a mammal, preferably a human, of greater than that of an unmodified antibody. Said antibody half life may be greater than about 15 days, greater than about 20 days, greater than about 25 days, greater than about 30 days, greater than about 35 days, greater than about 40 days, greater than about 45 days, greater than about 2 months, greater than about 3 months, greater than about 4 months, or greater than about 5 months. The increased half-lives of the antibodies of the present invention or fragments thereof in a mammal, preferably a human, result in a higher serum titer of said antibodies or antibody fragments in the mammal, and thus, reduce the frequency of the administration of said antibodies or antibody fragments and/or reduces the concentration of said antibodies or antibody fragments to be administered. Antibodies or fragments thereof having increased in vivo half-lives can be generated by techniques known to those of skill in the art. For example, antibodies or fragments thereof with increased in vivo half-lives can be generated by modifying (e.g., substituting, deleting or adding) amino acid residues identified as involved in the interaction between the Fc domain and the FcRn receptor (see, e.g., International Publication Nos. WO 97/34631 and WO 02/060919, which are incorporated herein by reference in their entireties). Antibodies or fragments thereof with increased in vivo half-lives can be generated by attaching to said antibodies or antibody fragments polymer molecules such as high molecular weight polyethyleneglycol (PEG). PEG can be attached to said antibodies or antibody fragments with or without a multifunctional linker either through site-specific conjugation of the PEG to the N- or C-terminus of said antibodies or antibody fragments or via epsilon-amino groups present on lysine residues. Linear or branched polymer derivatisation that results in minimal loss of biological activity will be used. The degree of conjugation will be closely monitored by SDS-PAGE and mass spectrometry to ensure proper conjugation of PEG molecules to the antibodies. Unreacted PEG can be separated from antibody-PEG conjugates by, e.g., size exclusion or ion-exchange chromatography. 
     As will be appreciated by one of skill in the art, in the above embodiments, while affinity values can be important, other factors can be as important or more so, depending upon the particular function of the antibody. For example, for an immunotoxin (toxin associated with an antibody), the act of binding of the antibody to the target can be useful; however, in some embodiments, it is the internalisation of the toxin into the cell that is the desired end result. As such, antibodies with a high percent internalisation can be desirable in these situations. Thus, in one embodiment, antibodies with a high efficiency in internalisation are contemplated. A high efficiency of internalisation can be measured as a percent internalised antibody, and can be from a low value to 100%. For example, in varying embodiments, 0.1-5, 5-10, 10-20, 20-30, 30-40, 40-45, 45-50, 50-60, 60-70, 70-80, 80-90, 90-99, and 99-100% can be a high efficiency. As will be appreciated by one of skill in the art, the desirable efficiency can be different in different embodiments, depending upon, for example, the associated agent, the amount of antibody that can be administered to an area, the side effects of the antibody-agent complex, the type (e.g., cancer type) and severity of the problem to be treated. 
     In other embodiments, the antibodies disclosed herein provide an assay kit for the detection of KDR expression in mammalian tissues or cells in order to screen for a disease or disorder associated with changes in expression of KDR. The kit comprises an antibody that binds KDR and means for indicating the reaction of the antibody with the antigen, if present. 
     Combinations 
     The targeted binding agent or antibody defined herein may be applied as a sole therapy or may involve, in addition to the compounds of the invention, conventional surgery or radiotherapy or chemotherapy. Such chemotherapy may include one or more of the following categories of anti tumour agents: 
     (i) other antiproliferative/antineoplastic drugs and combinations thereof, as used in medical oncology, such as alkylating agents (for example cis-platin, oxaliplatin, carboplatin, cyclophosphamide, nitrogen mustard, melphalan, chlorambucil, busulphan, temozolamide and nitrosoureas); antimetabolites (for example gemcitabine and antifolates such as fluoropyrimidines like 5-fluorouracil and tegafur, raltitrexed, methotrexate, cytosine arabinoside, and hydroxyurea); antitumor antibiotics (for example anthracyclines like adriamycin, bleomycin, doxorubicin, daunomycin, epirubicin, idarubicin, mitomycin-C, dactinomycin and mithramycin); antimitotic agents (for example vinca alkaloids like vincristine, vinblastine, vindesine and vinorelbine and taxoids like taxol and taxotere and polokinase inhibitors); and topoisomerase inhibitors (for example epipodophyllotoxins like etoposide and teniposide, amsacrine, topotecan and camptothecin); 
     (ii) cytostatic agents such as antioestrogens (for example tamoxifen, fulvestrant, toremifene, raloxifene, droloxifene and iodoxyfene), antiandrogens (for example bicalutamide, flutamide, nilutamide and cyproterone acetate), LHRH antagonists or LHRH agonists (for example goserelin, leuprorelin and buserelin), progestogens (for example megestrol acetate), aromatase inhibitors (for example as anastrozole, letrozole, vorazole and exemestane) and inhibitors of 5α-reductase such as finasteride; 
     (iii) anti-invasion agents (for example c-Src kinase family inhibitors like 4-(6-chloro-2,3-methylenedioxyanilino)-7-[2-(4-methylpiperazin-1-yl)ethoxy]-5-tetrahydropyran-4-yloxyquinazoline (AZD0530; International Patent Application WO 01/94341) and N-(2-chloro-6-methylphenyl)-2-{6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-ylamino}thiazole-5-carboxamide (dasatinib, BMS-354825 ; J. Med. Chem.,  2004, 47, 6658-6661), and metalloproteinase inhibitors like marimastat, inhibitors of urokinase plasminogen activator receptor function or, inhibitors of cathepsins, inhibitors of serine proteases for example matriptase, hepsin, urokinase, inhibitors of heparanase); 
     (iv) cytotoxic agents such as fludarabine, 2-chlorodeoxyadenosine, chlorambucil or doxorubicin and combination thereof such as Fludarabine+cyclophosphamide, CVP: cyclophosphamide+vincristine+prednisone, ACVBP: doxorubicin+cyclophosphamide+vindesine+bleomycin+prednisone, CHOP: cyclophosphamide+doxorubicin+vincristine+prednisone, CNOP: cyclophosphamide+mitoxantrone+vincristine+prednisone, m-BACOD: methotrexate+bleomycin+doxorubicin+cyclophosphamide+vincristine+dexamethasone+leucovorin., MACOP-B: methotrexate+doxorubicin+cyclophosphamide+vincristine+prednisone fixed dose+bleomycin+leucovorin, or ProMACE CytaBOM: prednisone+doxorubicin+cyclophosphamide+etoposide+cytarabine+bleomycin+vincristine+methotrexate+leucovorin. 
     (v) inhibitors of growth factor function, for example such inhibitors include growth factor antibodies and growth factor receptor antibodies (for example the anti-erbB2 antibody trastuzumab [Herceptin™], the anti-EGFR antibody panitumumab, the anti-erbB1 antibody cetuximab [Erbitux, C225] and any growth factor or growth factor receptor antibodies disclosed by Stern et al. Critical reviews in oncology/haematology, 2005, Vol. 54, pp 11-29); such inhibitors also include tyrosine kinase inhibitors, for example inhibitors of the epidermal growth factor family (for example EGFR family tyrosine kinase inhibitors such as N-(3-chloro-4-fluorophenyl)-7-methoxy-6-(3-morpholinopropoxy)quinazolin-4-amine (gefitinib, ZD1839), N-(3-ethynylphenyl)-6,7-bis(2-methoxyethoxy)quinazolin-4-amine (erlotinib, OSI-774) and 6-acrylamido-N-(3-chloro-4-fluorophenyl)-7-(3-morpholinopropoxy) -quinazolin-4-amine (CI 1033), erbB2 tyrosine kinase inhibitors such as lapatinib, inhibitors of the hepatocyte growth factor family, inhibitors of the platelet-derived growth factor family such as imatinib, inhibitors of serine/threonine kinases (for example Ras/Raf signalling inhibitors such as farnesyl transferase inhibitors, for example sorafenib (BAY 43-9006)), inhibitors of cell signalling through MEK and/or AKT kinases, inhibitors of the hepatocyte growth factor family, c-kit inhibitors, abl kinase inhibitors, IGF receptor (insulin-like growth factor) kinase inhibitors, aurora kinase inhibitors (for example AZD1152, PH739358, VX-680, MLN8054, R763, MP235, MP529, VX-528 and AX39459), cyclin dependent kinase inhibitors such as CDK2 and/or CDK4 inhibitors, and inhibitors of survival signaling proteins such as Bcl-2, Bcl-XL for example ABT-737; 
     (vi) antiangiogenic agents such as those which inhibit the effects of vascular endothelial growth factor, [for example the anti-vascular endothelial cell growth factor antibody bevacizumab (Avastin™) and VEGF receptor tyrosine kinase inhibitors such as 4-(4-bromo-2-fluoroanilino)-6-methoxy-7-(1-methylpiperidin-4-ylmethoxy)quinazoline (ZD6474; Example 2 within WO 01/32651), 4-(4-fluoro-2-methylindol-5-yloxy)-6-methoxy-7-(3-pyrrolidin-1-ylpropoxy)quinazoline (AZD2171; Example 240 within WO 00/47212), vatalanib (PTK787; WO 98/35985) and SU11248 (sunitinib; WO 01/60814), compounds such as those disclosed in International Patent Applications WO97/22596, WO 97/30035, WO 97/32856, WO 98/13354, WO00/47212 and WO01/32651 and compounds that work by other mechanisms (for example linomide, inhibitors of integrin αvβ3 function and angiostatin)] or colony stimulating factor 1 (CSF1) or CSF1 receptor.; 
     (vii) vascular damaging agents such as Combretastatin A4 and compounds disclosed in International Patent Applications WO 99/02166, WO 00/40529, WO 00/41669, WO 01/92224, WO 02/04434 and WO 02/08213; 
     (viii) antisense therapies, for example those which are directed to the targets listed above, such as G-3139 (Genasense), an anti bcl2 antisense; 
     (ix) gene therapy approaches, including for example approaches to replace aberrant genes such as aberrant p53 or aberrant BRCA1 or BRCA2, GDEPT (gene directed enzyme pro drug therapy) approaches such as those using cytosine deaminase, thymidine kinase or a bacterial nitroreductase enzyme and approaches to increase patient tolerance to chemotherapy or radiotherapy such as multi drug resistance gene therapy; and 
     (x) immunotherapy approaches, including for example treatment with Alemtuzumab (campath-1H™), a monoclonal antibody directed at CD52, or treatment with antibodies directed at CD22, ex vivo and in vivo approaches to increase the immunogenicity of patient tumour cells, transfection with cytokines such as interleukin 2, interleukin 4 or granulocyte macrophage colony stimulating factor, approaches to decrease T cell anergy such as treatment with monoclonal antibodies inhibiting CTLA-4 function, approaches using transfected immune cells such as cytokine transfected dendritic cells, approaches using cytokine transfected tumour cell lines and approaches using anti idiotypic antibodies. 
     (xi) inhibitors of protein degradation such as proteasome inhibitor such as Velcade (bortezomid). 
     (xii) biotherapeutic therapeutic approaches for example those which use peptides or proteins (such as antibodies or soluble external receptor domain constructions) which either sequester receptor ligands, block ligand binding to receptor or decrease receptor signalling (e.g. to due to enhanced receptor degradation or lowered expression levels). 
     In one embodiment the anti-tumour treatment defined herein may involve, in addition to the compounds of the invention, treatment with other antiproliferative/antineoplastic drugs and combinations thereof, as used in medical oncology, such as alkylating agents (for example cis-platin, oxaliplatin, carboplatin, cyclophosphamide, nitrogen mustard, melphalan, chlorambucil, busulphan, temozolamide and nitrosoureas); antimetabolites (for example gemcitabine and antifolates such as fluoropyrimidines like 5-fluorouracil and tegafur, raltitrexed, methotrexate, cytosine arabinoside, and hydroxyurea); antitumour antibiotics (for example anthracyclines like adriamycin, bleomycin, doxorubicin, daunomycin, epirubicin, idarubicin, mitomycin-C, dactinomycin and mithramycin); antimitotic agents (for example vinca alkaloids like vincristine, vinblastine, vindesine and vinorelbine and taxoids like taxol and taxotere and polokinase inhibitors); and topoisomerase inhibitors (for example epipodophyllotoxins like etoposide and teniposide, amsacrine, topotecan and camptothecin). 
     In one embodiment the anti-tumour treatment defined herein may involve, in addition to the compounds of the invention, treatment with gemcitabine. 
     Such conjoint treatment may be achieved by way of the simultaneous, sequential or separate dosing of the individual components of the treatment. Such combination products employ the compounds of this invention, or pharmaceutically acceptable salts thereof, within the dosage range described hereinbefore and the other pharmaceutically active agent within its approved dosage range. 
     EXAMPLES 
     The following examples, including the experiments conducted and results achieved are provided for illustrative purposes only and are not to be construed as limiting upon the teachings herein. 
     Example 1 
     Immunization and Titering 
     Immunization 
     Immunizations were conducted using soluble KDR (VEGF Receptor 2 D1-7 , Cat. #676490, Calbiochem). For the campaign, 10 μg/mouse of soluble protein was provided in the initial boost, followed by 5 μg/mouse in subsequent boosts, for immunization in XenoMouse™. The immunization was carried out according to the methods disclosed in U.S. patent application Ser. No. 08/759,620, filed Dec. 3, 1996 and International Patent Application Nos. WO 98/24893, published Jun. 11, 1998 and WO 00/76310, published Dec. 21, 2000, the disclosures of which are hereby incorporated by reference. The immunization programs are summarized in Table 2. 
     Selection of Animals for Harvest by Titer 
     Titers of the antibody against human KDR were tested by FACS staining for native antigen binding using Human Umbilical Vein Endothelial Cells (HUVEC) or by ELISA assay for binding to immobilized soluble KDR. At the end of the immunization program, fusions were performed using mouse myeloma cells and lymphocytes isolated from the spleens and lymph nodes of the immunized mice by means of electroporation, as described in Example 2. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Summary of Immunization Programs 
               
            
           
           
               
               
               
               
               
               
            
               
                 Cam- 
                   
                   
                   
                 No of 
                   
               
               
                 paign 
                 Group 
                 Immunogen 
                 Strain 
                 mice 
                 Immunization routes 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 1 
                 1 
                 Soluble KDR 
                 IgG2 
                 10 
                 IP/Tail/BIP, twice/wk, 
               
               
                   
                   
                 (Calbiochem 
                   
                   
                 ×8 wks, followed by 
               
               
                   
                   
                 #676490) 
                   
                   
                 IP/Tail/BIP, once/every 2 
               
               
                   
                   
                   
                   
                   
                 weeks, × 6 wks 
               
               
                 1 
                 2 
                 Soluble KDR 
                 IgG4 
                 10 
                 IP/Tail/BIP, twice/wk, 
               
               
                   
                   
                 (Calbiochem 
                   
                   
                 ×8 wks, followed by 
               
               
                   
                   
                 #676490) 
                   
                   
                 IP/Tail/BIP, once/every 2 
               
               
                   
                   
                   
                   
                   
                 weeks, ×6 wks 
               
               
                   
               
               
                 “IP” refers to “intraperitoneal” 
               
               
                 “BIP” refers to “Base of Tail/Intraperitoneal” 
               
            
           
         
       
     
     Example 2 
     Recovery of Lymphocytes, B-Cell Isolations, Fusions and Generation of Hybridomas 
     Immunized mice were sacrificed by cervical dislocation, and the draining lymph nodes harvested and pooled from each cohort. There were two harvests performed for this program. Harvest 1 used six mice with ID numbers 157166, 157168, 157597, 157599, 157600, 157602. Harvest 2 used five mice with ID numbers 157662, 157663, 157665, 157672, 157694. 
     The lymphoid cells were dissociated by grinding in DMEM to release the cells from the tissues and the cells were suspended in DMEM. The cells were counted, and 0.9 ml DMEM per 100 million lymphocytes added to the cell pellet to resuspend the cells gently but completely. Using 100 μl of CD90+magnetic beads per 100 million cells, the cells were labeled by incubating the cells with the magnetic beads at 4° C. for 15 minutes. The magnetically labeled cell suspension containing up to 10 8  positive cells (or up to 2×10 9  total cells) was loaded onto a LS+column and the column washed with DMEM. The total effluent was collected as the CD90-negative fraction (most of these cells were expected to be B cells). 
     The fusion was performed by mixing washed enriched Day 6 B cells with nonsecretory myeloma P3X63Ag8.653 cells purchased from ATCC, cat. # CRL 1580 (Kearney et al, J. Immunol. 123, 1979, 1548-1550) at a ratio of 1:4. The cell mixture was gently pelleted by centrifugation at 400×g for 4 minutes. After decanting of the supernatant, the cells were gently mixed using a 1 ml pipette. Preheated PEG (1 ml per 10 6  B-cells) was slowly added with gentle agitation over 1 minute followed by 1 minute of mixing. Preheated IDMEM (2 ml per 10 6  B-cells) was then added over 2 minutes with gentle agitation. Finally preheated IDMEM (8 ml per 10 6  B-cells) was added over 3 minutes. 
     The fused cells were spun down at 400×g for 6 minutes and resuspended in 20 ml of Selection media (DMEM (Invitrogen), 15% FBS (Hyclone), supplemented with L-glutamine, pen/strep, MEM Non-essential amino acids, Sodium Pyruvate, 2-Mercaptoethanol (all from Invitrogen), HA-Azaserine Hypoxanthine and OPI (oxaloacetate, pyruvate, bovine insulin) (both from Sigma) and IL-6 (Boehringer Mannheim)) per 10 6  B-cells. Cells were incubated for 20-30 minutes at 37° C. and then resuspended in 200 ml Selection media and cultured for 3-4 days in a T175 flask. 
     Day 3 post fusion the cells were collected, spun for 8 minutes at 400×g and resuspended in 10 ml Selection media per 10 6  fused B-cells. FACS analysis of hybridoma population was performed, and cells were subsequently frozen down. 
     Hybridomas were grown as routine in the selective medium. Exhaustive supernatants collected from the hybridomas that potentially produce anti-human KDR antibodies were subjected to subsequent screening assays. 
     Example 3 
     Antibody Titer Measurement 
     Native Antigen Binding of Human Umbilical Vein Endothelial Cells (HUVEC) 
     FACS analysis was performed on human umbilical vein endothelial cells (HUVECs) to measure the titers of antibody against human KDR produced as described in Examples 1 and 2. HUVECs were seeded at 60,000 cells/well and incubated with 2 μL of sample (at 1:50 dilution) for one hour at 4° C. The wells were then washed and incubated with Cy5-conjugated goat anti-human antibody at 5 μg/mL and 7-Amino-Actinomycin (7AAD) at 5 μg/mL for 15 minutes at 4° C. Bound KDR was detected using FACS analysis. The positive control was goat anti-KDR antibody (R&amp;D Systems, Inc.), and negative controls included negative controls included naïve XMG2 sera and naïve XMG4 (XM3C-1) sera. Animals with the greatest FACS Geometric Mean Fluorescence were selected for subsequent hybridoma generation. Table 3 lists the FACS data obtained from analysis of the HUVECs. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Titers of antibody against human KDR as 
               
               
                 measured by FACS analysis of HUVECs 
               
            
           
           
               
               
            
               
                 Mouse 
                 FACS Geometric Mean 
               
               
                   
               
            
           
           
               
               
            
               
                 P157166 
                 18.97 
               
               
                 P157168 
                 15.82 
               
               
                 P157169 
                 10.43 
               
               
                 P157597 
                 24.34 
               
               
                 P157598 
                 11.29 
               
               
                 P157599 
                 19.66 
               
               
                 P157600 
                 42.11 
               
               
                 P157602 
                 33.2 
               
               
                 P157661 
                 10.64 
               
               
                 P157662 
                 20.18 
               
               
                 P157663 
                 24.63 
               
               
                 P157664 
                 7.59 
               
               
                 P157665 
                 17.25 
               
               
                 P157672 
                 28.64 
               
               
                 P157692 
                 12.94 
               
               
                 P157693 
                 11.1 
               
               
                 P157694 
                 31.69 
               
               
                 P157695 
                 7.71 
               
               
                 Naïve G2 
                 3.73 
               
               
                 Naïve G4 
                 3.06 
               
               
                 Control: Goat anti-KDR (2 μg/mL) 
                 21.68 
               
               
                 Control: Goat anti-KDR (0.2 μg/mL) 
                 8.23 
               
               
                 Control: Goat anti-KDR (0.02 μg/mL) 
                 4.25 
               
               
                 Control: Goat isotype (2 μg/mL) 
                 3.28 
               
               
                   
               
            
           
         
       
     
     Example 4 
     Antibody Titer Measurement  
     Binding to Immobilized Soluble KDR 
     ELISA assay of binding to immobilized soluble KDR was used to measure titers of antibody produced as described in Examples 1 and 2. 
     To measure the titers of antibody, plates were coated with KDR (Calbiochem) at 1 μg/mL overnight at 4° C. Serum containing antibodies were then titrated 1:3 in duplicate starting at 1:100 dilution in blocking buffer (1×PBS/1% milk). The plates containing sera were incubated for one hour at room temperature, washed, and subsequently incubated with secondary antibody (goat anti-human IgG Fc POD—Jackson Laboratories) for one hour at room temperature. The positive control was goat anti-KDR (Catalog #AF357, R&amp;D Systems, Inc.), and negative controls included naïve XMG2 sera and naïve XMG4 (XM3C-1) sera. Table 4 provides a summary of the ELISA readings obtained from analysis of the bound antibodies. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Titers of antibody against immobilized 
               
               
                 soluble KDR as measured by ELISA assay 
               
            
           
           
               
               
               
            
               
                   
                 Mouse 
                 Coating: KDR @ 1 μg/mL 
               
               
                   
               
            
           
           
               
               
               
            
               
                   
                 P157166 
                 13400 
               
               
                   
                 P157168 
                 14144 
               
               
                   
                 P157169 
                 2309 
               
               
                   
                 P157597 
                 11027 
               
               
                   
                 P157598 
                 2080 
               
               
                   
                 P157599 
                 21053 
               
               
                   
                 P157600 
                 11507 
               
               
                   
                 P157602 
                 67697 
               
               
                   
                 P157661 
                 2393 
               
               
                   
                 P157662 
                 3207 
               
               
                   
                 P157663 
                 6816 
               
               
                   
                 P157664 
                 2171 
               
               
                   
                 P157665 
                 2488 
               
               
                   
                 P157672 
                 7197 
               
               
                   
                 P157692 
                 7060 
               
               
                   
                 P157693 
                 2340 
               
               
                   
                 P157694 
                 6568 
               
               
                   
                 P157695 
                 2051 
               
               
                   
                 Naïve G2 
                 &lt;100 @ OD 0.217 
               
               
                   
                 Naïve G4 
                 &lt;100 @ OD 0.244 
               
               
                   
               
            
           
         
       
     
     Example 5 
     Hybridoma Supernatant Screening by Binding Assay 
     Hybridoma supernatants containing antibody, produced as described in Examples 1 and 2, were screened by assays that measure binding to both immobilized soluble human KDR and to the Ig4-7 domain of KDR. 
     To screen by binding to immobilized soluble KDR, plates were coated with KDR (Calbiochem) at 250 ng/mL overnight at 4° C. After blocking and washing the coated plates, the candidate hybridoma supernatants (at 1:5 dilution) were transferred to the plates and incubated for one hour at room temperature. The plates were then washed and incubated with secondary antibody (at 1:4000 dilution) for one hour at room temperature. Table 5 provides a summary of the ELISA readings obtained from analysis of the bound antibodies for the screening. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 ELISA results (substrate: immobilized soluble human KDR) 
               
            
           
           
               
               
               
            
               
                   
                 MAb ID 
                 KDR OD(450 nm) 
               
               
                   
               
               
                   
                 1G6 
                 6.000 
               
               
                   
                 21A1 
                 0.967 
               
               
                   
                 21H6 
                 0.620 
               
               
                   
                 21H9 
                 1.647 
               
               
                   
                 22B8 
                 1.183 
               
               
                   
                 24B3 
                 6.000 
               
               
                   
                 24C9 
                 0.550 
               
               
                   
                 27A3 
                 3.749 
               
               
                   
                 27D10 
                 3.238 
               
               
                   
                 29A3 
                 0.814 
               
               
                   
                 29H3 
                 0.830 
               
               
                   
                 29D4 
                 1.001 
               
               
                   
                 29F7 
                 1.222 
               
               
                   
                 29A11 
                 3.527 
               
               
                   
                 30A1 
                 0.786 
               
               
                   
                 30E3 
                 4.178 
               
               
                   
                 30F6 
                 0.454 
               
               
                   
                 30D7 
                 3.448 
               
               
                   
                 30H10 
                 0.943 
               
               
                   
                 31E11 
                 1.137 
               
               
                   
                 32B2 
                 1.111 
               
               
                   
                 32F4 
                 0.907 
               
               
                   
                 32G7 
                 1.066 
               
               
                   
                 32C11 
                 0.924 
               
               
                   
                 33B1 
                 1.111 
               
               
                   
                 33E1 
                 4.555 
               
               
                   
                 33C3 
                 1.154 
               
               
                   
                 33D5 
                 3.587 
               
               
                   
               
            
           
         
       
     
     For this experiment, a KDR antibody containing the Ig4-7 domain (b-KDRIg4-7-myc-his) was obtained by cloning the extracellular domains 4-7 of the KDR antibody into pSecTag DNA expression constructs. The constructs were transiently transfected and expressed in 293T cells, and the expressed his-tagged protein was purified by nickel (Ni) column chromatography. 
     To screen by binding to the Ig4-7 domain of KDR, plates were first coated with neutravadin (Pierce) at 4 μg/mL and incubated overnight at 4° C. The plates were then blocked and subsequently incubated with b-KDRIg4-7-myc-his at 1 μg/mL for one hour at room temperature. Candidate hybridoma supernatants (at 1:5 dilution) were transferred to the plates and incubated for one hour at room temperature. The plates were then washed and incubated with goat anti-human IgG Fc POD (at 1:4000 dilution) for one hour at room temperature. Table 6 provides a summary of the ELISA readings obtained from analysis of the bound antibodies for the screening. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 ELISA results (substrate: Ig4-7 domain of KDR) 
               
            
           
           
               
               
               
            
               
                   
                 MAb ID 
                 b-KDRIg4-7 OD(450 nm) 
               
               
                   
               
               
                   
                 1G6 
                 0.056 
               
               
                   
                 21A1 
                 1.274 
               
               
                   
                 21H6 
                 0.935 
               
               
                   
                 21H9 
                 0.052 
               
               
                   
                 22B8 
                 2.815 
               
               
                   
                 24B3 
                 0.065 
               
               
                   
                 24C9 
                 0.066 
               
               
                   
                 27A3 
                 1.820 
               
               
                   
                 27D10 
                 2.031 
               
               
                   
                 29A3 
                 0.201 
               
               
                   
                 29H3 
                 0.071 
               
               
                   
                 29D4 
                 2.608 
               
               
                   
                 29F7 
                 0.082 
               
               
                   
                 29A11 
                 0.062 
               
               
                   
                 30A1 
                 1.209 
               
               
                   
                 30E3 
                 0.049 
               
               
                   
                 30F6 
                 0.093 
               
               
                   
                 30D7 
                 0.056 
               
               
                   
                 30H10 
                 0.047 
               
               
                   
                 31E11 
                 1.279 
               
               
                   
                 32B2 
                 0.061 
               
               
                   
                 32F4 
                 2.291 
               
               
                   
                 32G7 
                 0.068 
               
               
                   
                 32C11 
                 0.050 
               
               
                   
                 33B1 
                 0.058 
               
               
                   
                 33E1 
                 0.620 
               
               
                   
                 33C3 
                 3.659 
               
               
                   
                 33D5 
                 0.107 
               
               
                   
               
            
           
         
       
     
     Example 6 
     Cross-Reactivity to Human VEGF-Receptor-1 (Flt-1) and Mouse KDR (Flk-1) 
     The cross-reactivity of antibodies in the hybridoma supernatant was tested against KDR mouse ortholog (mouse VEGFR2, or Flk-1) and human VEGFR1 (Flt-1). Cross-reactivity was measured using an ELISA based assay. Cross-reactivity to KDR mouse ortholog (Flk-1) was desired. However, antibodies that selected over Flt-1 were chosen for further analysis. 
     Plates were coated with human VEGFR1 (Flt-1)/Fc (Flt-1, Cat. #321-FL/CF, R&amp;D Systems, Inc.) or mouse KDR (Flk-1)/Fc (Flk-1, Cat. #443-KD/CF, R&amp;D Systems, Inc.) at 500 ng/mL and incubated overnight at 4° C. After washing and blocking the plates, the antibody-containing supernatants were added (at 1:5 dilution) into the coated wells and incubated for one hour at room temperature. The plates were then incubated with mouse anti-human IgG 2, 3 and 4 and subsequently with goat anti-mouse IgG Fc POD, to detect and measure bound antibodies. Table 7 provides the results of the ELISA cross-reactivity assay for each substrate. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 ELISA Cross-Reactivity Assay Results Against 
               
               
                 Mouse KDR (Flk-1) and Human VEGFR1 (Flt-1) 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Flk-1 (mouse KDR), 
                 Flt-1 (human VEGFR1), 
               
               
                   
                 MAb ID 
                 OD (450 nm) 
                 OD (450 nm) 
               
               
                   
               
               
                   
                 1G6 
                 0.055 
                 0.054 
               
               
                   
                 21A1 
                 0.270 
                 0.180 
               
               
                   
                 21H6 
                 0.193 
                 0.086 
               
               
                   
                 21H9 
                 0.139 
                 0.134 
               
               
                   
                 22B8 
                 0.074 
                 0.078 
               
               
                   
                 24B3 
                 0.094 
                 0.068 
               
               
                   
                 24C9 
                 0.098 
                 0.080 
               
               
                   
                 27A3 
                 0.156 
                 0.068 
               
               
                   
                 27D10 
                 0.166 
                 0.140 
               
               
                   
                 29A3 
                 0.416 
                 0.069 
               
               
                   
                 29H3 
                 0.096 
                 0.071 
               
               
                   
                 29D4 
                 0.100 
                 0.079 
               
               
                   
                 29F7 
                 0.093 
                 0.061 
               
               
                   
                 29A11 
                 0.261 
                 0.080 
               
               
                   
                 30A1 
                 0.107 
                 0.067 
               
               
                   
                 30E3 
                 0.112 
                 0.058 
               
               
                   
                 30F6 
                 0.067 
                 0.075 
               
               
                   
                 30D7 
                 0.135 
                 0.062 
               
               
                   
                 30H10 
                 0.338 
                 0.097 
               
               
                   
                 31E11 
                 0.162 
                 0.134 
               
               
                   
                 32B2 
                 0.274 
                 0.114 
               
               
                   
                 32F4 
                 0.094 
                 0.090 
               
               
                   
                 32G7 
                 0.092 
                 0.085 
               
               
                   
                 32C11 
                 0.083 
                 0.063 
               
               
                   
                 33B1 
                 0.108 
                 0.085 
               
               
                   
                 33E1 
                 0.097 
                 0.075 
               
               
                   
                 33C3 
                 0.076 
                 0.067 
               
               
                   
                 33D5 
                 0.201 
                 0.093 
               
               
                   
               
            
           
         
       
     
     Example 7 
     Native Binding to Human Umbilical Vein Endothelial Cells (HUVECs) 
     FACS analysis was performed on human umbilical vein endothelial cells (HUVECs) to confirm native binding of antibody in the hybrodima supernatant produced as described in Examples 1 and 2. HUVECs were seeded at 15,000 cells/well and incubated with 100 μL of sample supernatant (at 1:50 dilution) for one hour at 4° C. The positive control was goat anti-KDR antibody (R&amp;D Systems, Inc.), and negative controls included irrelevant hybridoma supernatants at the same dilution. The cells were then washed and incubated with Cy5-conjugated goat anti-human antibody at 5 μg/mL and 7-Amino-Actinomycin D (7AAD) at 5 μg/mL for 15 minutes at 4° C. Bound KDR was detected using FACS analysis. Table 8 lists the FACS data obtained from analysis of the HUVECs. Antibodies with strong binding to HUVEC cells were considered as having higher relative avidity. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Native binding to HUVECs as measured by FACS 
               
            
           
           
               
               
               
            
               
                   
                   
                 FACS Geometric Mean 
               
               
                   
                   
                 (1:50 dilution of 
               
               
                   
                 MAb ID 
                 hybridoma supernatant) 
               
               
                   
               
               
                   
                 1G6 
                 32 
               
               
                   
                 21A1 
                 14 
               
               
                   
                 21H6 
                 18 
               
               
                   
                 21H9 
                 29 
               
               
                   
                 22B8 
                 24 
               
               
                   
                 24B3 
                 26 
               
               
                   
                 24C9 
                 33 
               
               
                   
                 27A3 
                 25 
               
               
                   
                 27D10 
                 24 
               
               
                   
                 29A3 
                 30 
               
               
                   
                 29H3 
                 27 
               
               
                   
                 29D4 
                 26 
               
               
                   
                 29F7 
                 32 
               
               
                   
                 29A11 
                 31 
               
               
                   
                 30A1 
                 25 
               
               
                   
                 30E3 
                 36 
               
               
                   
                 30F6 
                 21 
               
               
                   
                 30D7 
                 31 
               
               
                   
                 30H10 
                 31 
               
               
                   
                 31E11 
                 18 
               
               
                   
                 32B2 
                 26 
               
               
                   
                 32F4 
                 27 
               
               
                   
                 32G7 
                 28 
               
               
                   
                 32C11 
                 26 
               
               
                   
                 33B1 
                 25 
               
               
                   
                 33E1 
                 14 
               
               
                   
                 33C3 
                 20 
               
               
                   
                 33D5 
                 24 
               
               
                   
               
            
           
         
       
     
     Example 8 
     Determination of Relative Potency of Antibody-Containing Supernatants 
     The relative potency of the various antibody-containing supernatants was compared by measuring how well the antibodies blocked KDR phosphorylation in an endogenously KDR-expressing normal cell line (HUVEC). The assays were conducted using a supernatant dilution of 1:20 and included examination of the blockade of both VEGF165-mediated and VEGF-E mediated KDR tyrosine phosphorylation. VEGF165 binds to both KDR:KDR homodimers and to KDR:VEGF-Receptor-1 hetrodimers. VEGF-E is an Orf virus homologue of VEGF165; however, unlike VEGF165, VEGF-E is specifically binds to KDR, therefore providing selective analysis of KDR dimerization ( Endocrine Reviews , August 2004, 25(4): 581-611). 
     The relative potencies of the different antibody containing supernatants were also examined for their ability to block survival of serum-deprived HUVEC cells as mediated by both VEGF165 and VEGF-E. These assays were also performed at a supernatant dilution of 1:20. All incubations with HUVEC cells took place at 37° C. and 5% CO 2 . 
     Assay to Measure Inhibition of VEGF165-Mediated KDR Activity 
     HUVEC cells were seeded at 25,000 cells/well and incubated overnight in Endothelial Cell Basal Medium-2 (EBM-2, Clonetics EGM-2 BulletKit, catalog #CC-3162)+2% FCS+the following growth supplements: hydrocortisone, hFGF-B, R3-IGF-1, ascorbic acid, heparin, FBS, hEGF, and GA-1000. The cells were then washed and incubated in supplement-free media overnight. On the third day, the various antibody-containing supernatants were added to the HUVEC cells and incubated for 2 hours. The supernatant volumes were then removed and replaced by 50 μL of VEGF165 at 2 nM final concentration. After incubation with VEGF165 for 7 minutes, the cells were lysed, and the cell lysates were measured for inhibition of VEGF165-mediated KDR activity. Table 9 provides a listing of average (n=3) ELISA readings indicating the level of inhibition of VEGF165-mediated KDR tyrosine phosphorylation. 
                     TABLE 9                  Inhibition of VEGF165-mediated KDR tyrosine phosphorylation in       HUVEC cells                              % Inhibition of VEGF165-mediated pTyr Activity                                             MAb ID   n = 1   n = 2   n = 3   Average   St. Dev.   % CV                                                 1G6   89   67   58   71   16   23       21A1   71   77   68   72   5   7       21H6   85   65   68   72   11   15       21H9   80   70   61   70   9   13       22B8   82   65   53   67   15   22       24B3   69   51   33   51   18   35       24C9   55   45   38   46   8   18       27A3   60   68   59   62   5   8       27D10   62   48   63   58   8   15       29A3   92   66   72   77   14   18       29H3   89   72   66   76   12   16       29D4   85   67   63   72   12   16       29F7   90   67   77   78   12   15       29A11   96   72   66   78   16   20       30A1   86   59   52   65   18   27       30E3   89   67   58   71   16   23       30F6   98   60   52   70   24   35       30D7   99   71   59   76   21   27       30H10   92   77   59   76   17   22       31E11   81   80   59   74   12   17       32B2   91   75   66   77   13   17       32F4   88   76   56   73   16   22       32G7   73   47   11   44   31   71       32C11   84   75   57   72   14   19       33B1   76   56   31   54   23   42       33E1   88   78   52   73   19   26       33C3   87   75   50   71   19   27       33D5   95   72   63   77   16   21                    
Assay to Measure Inhibition of VEGF-E-Mediated KDR Activity
 
     HUVEC cells were seeded and incubated with antibody-containing supernatants as described above. The supernatant volumes were then removed and replaced by 50 μL of VEGF-E at 2 nM final concentration. After incubation with VEGF-E for 7 minutes, the cells were lysed, and the cell lysates were measured for inhibition of VEGF-E-mediated KDR activity. Table 10 provides a listing of ELISA readings indicating the level of inhibition of VEGF-E-mediated KDR tyrosine phosphorylation. 
                     TABLE 10                  Inhibition of VEGF-E-mediated KDR tyrosine       phosphorylation in HUVEC cells                                 % Inhibition of               VEGF-E-mediated           MAb ID   pTyr Activity                                          1G6   24           21A1   67           21H6   70           21H9   6           22B8   61           24B3   40           24C9   51           27A3   60           27D10   63           29A3   22           29H3   18           29D4   67           29F7   45           29A11   15           30A1   50           30E3   19           30F6   56           30D7   24           30H10   20           31E11   67           32B2   15           32F4   62           32G7   57           32C11   17           33B1   54           33E1   46           33C3   66           33D5   30                        
Assay to Measure Ability of Antibody to Block Survival of Serum-Deprived HUVEC Cells as Mediated by VEGF165
 
     HUVEC cells were seeded at 10,000 cells/well and incubated overnight in EBM-2+2% FCS+all supplements except VEGF (see above). The cells were then washed and the various antibody-containing supernatants (at either 1:10 or 1:20 dilution in 50 μL of basal EBM-2 media) were added to the HUVEC cells and incubated for 2 hours. Fifty (50) μL of VEGF165 (1 nM final concentration) with chloroquine (25 nM final concentration, Sigma-Aldrich) and 0.01% FCS (final concentration) was added to the cells, and the cells were incubated for 4 days at 37° C. and 5% CO 2 . Cell survival was then measured by addition of luminescent substrate (Cell Titer Glo, Promega) per manufacturer&#39;s protocol and detected using a luminometer. Antibody inhibition of HUVEC survival is indicated by lower Relative Luminscence Unit (RLU) values. Table 11 provides a listing of (n=2) luminometer readings indicating HUVEC survival by VEGF165-mediated KDR activity. 
                     TABLE 11                  Survival of HUVEC Cells by VEGF165-mediated KDR Activity                                     HUVEC survival (n = 1)   HUVEC survival (n = 2)               1:10 dilution of   1:20 dilution of           MAb ID   supernatant (RLU)   supernatant (RLU)                                              1G6   47799   10197           21A1   8871   2673           21H6   10200   247           21H9   43793   10269           22B8   12860   3260           24B3   14148   2459           24C9   14615   9180           27A3   17291   4563           27D10   20114   9713           29A3   36363   2450           29H3   32652   544           29D4   12886   5384           29F7   44127   23984           29A11   34648   27654           30A1   14389   9728           30E3   37528   30447           30F6   14832   1348           30D7   28288   2524           30H10   39549   21110           31E11   9135   6979           32B2   19290   400           32F4   20111   5026           32G7   24875   11898           32C11   44831   8574           33B1   19422   12454           33E1   25742   2308           33C3   6539   4708           33D5   43395   706                        
Assay to Measure Ability of Antibody to Block Survival of Serum-Deprived HUVEC Cells as Mediated by VEGF-E
 
     HUVEC cells were seeded and incubated with antibody-containing supernatants as described above. The cells were then washed and the various antibody-containing supernatants (at 1:10 dilution in basal EBM-2 media) were added to the HUVEC cells and incubated for 2 hours. VEGF-E (Cederlane) was added to the cells to a final concentration of 1 nM, and the cells were incubated for 4 days. Cell survival was then measured as above by addition of luminescent substrate and luminometer assay. Table 12 provides a listing of the degree of inhibition observed as a percentage of VEGF-E maximal activity. Values approaching 100% are equivalent to complete inhibition of VEGF-E activity. 
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 Survival of HUVEC Cells by VEGF-E-mediated KDR Activity 
               
            
           
           
               
               
               
            
               
                   
                 VEGF-E-mediated 
                   
               
               
                   
                 Survival % Inhibition 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 MAb ID 
                 n = 1 
                 n = 2 
                 Average 
                 St. Dev. 
                 % CV 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 1G6 
                 −2 
                 6 
                 2 
                 6 
                 324 
               
               
                   
                 21A1 
                 45 
                 40 
                 42 
                 4 
                 8 
               
               
                   
                 21H6 
                 56 
                 53 
                 55 
                 2 
                 4 
               
               
                   
                 21H9 
                 −3 
                 1 
                 −1 
                 3 
                 −506 
               
               
                   
                 22B8 
                 57 
                 53 
                 55 
                 3 
                 5 
               
               
                   
                 24B3 
                 65 
                 66 
                 66 
                 1 
                 1 
               
               
                   
                 24C9 
                 72 
                 72 
                 72 
                 1 
                 1 
               
               
                   
                 27A3 
                 63 
                 52 
                 58 
                 8 
                 13 
               
               
                   
                 27D10 
                 50 
                 64 
                 57 
                 10 
                 18 
               
               
                   
                 29A3 
                 −1 
                 −20 
                 −10 
                 13 
                 −129 
               
               
                   
                 29H3 
                 −19 
                 −11 
                 −15 
                 6 
                 −42 
               
               
                   
                 29D4 
                 64 
                 67 
                 66 
                 2 
                 3 
               
               
                   
                 29F7 
                 15 
                 6 
                 11 
                 6 
                 60 
               
               
                   
                 29A11 
                 8 
                 1 
                 5 
                 5 
                 112 
               
               
                   
                 30A1 
                 58 
                 59 
                 58 
                 1 
                 2 
               
               
                   
                 30E3 
                 −21 
                 −7 
                 −14 
                 10 
                 −68 
               
               
                   
                 30F6 
                 31 
                 43 
                 37 
                 8 
                 23 
               
               
                   
                 30D7 
                 −27 
                 −22 
                 −24 
                 4 
                 −14 
               
               
                   
                 30H10 
                 −21 
                 2 
                 −10 
                 17 
                 −175 
               
               
                   
                 31E11 
                 56 
                 54 
                 55 
                 1 
                 3 
               
               
                   
                 32B2 
                 −2 
                 −9 
                 −6 
                 5 
                 −83 
               
               
                   
                 32F4 
                 64 
                 64 
                 64 
                 0 
                 0 
               
               
                   
                 32G7 
                 69 
                 54 
                 62 
                 11 
                 18 
               
               
                   
                 32C11 
                 5 
                 7 
                 6 
                 2 
                 27 
               
               
                   
                 33B1 
                 66 
                 68 
                 67 
                 2 
                 2 
               
               
                   
                 33E1 
                 66 
                 54 
                 60 
                 9 
                 14 
               
               
                   
                 33C3 
                 78 
                 69 
                 74 
                 7 
                 9 
               
               
                   
                 33D5 
                 −2 
                 2 
                 0 
                 3 
                 −678 
               
               
                   
                   
               
            
           
         
       
     
     Example 9 
     Kinetic Assays 
     These experiments were conducted to identify high affinity/high potency antibodies in the hybridoma supernatants obtained as described in Example 2. The experiments were performed using soluble KDR extracellular domain (ECD) (Calbiochem). 
     High Antigen (HA) Quantitation (ELISA) 
     ELISA plates were coated with a greater amount of KDR (at 500 ng/mL) in comparison with the Limited Antigen Quantitation assay described below. Sample containing antibody (Ab) was titrated on the KDR-coated ELISA plates and was incubated overnight at 4° C. to allow Ab binding to approach equilibrium. Titration of Ab in sample covered a dilution range of 1:25 to 1:18,225. A standard curve of KDR-specific antibody of known concentration was used to define the linear range of the assay. Data within the linear range were used to derive the relative concentration of KDR-specific Ab in each titrated sample. The high KDR concentration and the overnight incubation limited the effect of Ab affinity, allowing quantitation of the relative amount of KDR-specific Ab present in each sample. 
     Limited Antigen (LA) Quantitation (ELISA) 
     ELISA plates were coated with a lower amount of KDR (3.125, 6.25, 12.5, 25 and 50 ng/mL) in comparison with the High Antigen Quantitation assay described above. Samples containing one concentration of antibody (Ab) (1:25 dilution) were incubated overnight to allow Ab binding to approach equilibrium. The low antigen concentration limited the effect of antibody concentration, allowing ranking of the antibodies based on their relative affinity. 
     Tables 13 and 14 summarize the results for the hybridoma lines that had the desired neutralizing activity and preferred binding kinetics. 
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 High Antigen Supernatant Dilution ELISA Assay Results 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 HA 
                   
                   
               
               
                   
                   
                 Derived 
               
               
                 MAb 
                 High Antigen Hybridoma Supernatant Dilution 
                 Conc. 
                 Std. 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 ID 
                 1:25 
                 1:75 
                 1:225 
                 1:675 
                 1:2025 
                 1:6075 
                 1:18,225 
                 (μg/mL) 
                 Dev. 
                 % CV 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 1G6 
                 6.000 
                 6.000 
                 4.490 
                 2.240 
                 0.690 
                 0.340 
                 0.260 
                 5.1 
                 1.5 
                 28.6 
               
               
                 21A1 
                 4.450 
                 4.430 
                 4.140 
                 3.360 
                 1.660 
                 0.560 
                 0.250 
                 13.0 
                 1.1 
                 8.6 
               
               
                 21H6 
                 6.000 
                 4.190 
                 5.570 
                 4.020 
                 2.910 
                 1.170 
                 0.540 
                 30.7 
                 3.9 
                 12.6 
               
               
                 21H9 
                 6.000 
                 4.340 
                 3.410 
                 1.560 
                 0.700 
                 0.260 
                 0.120 
                 3.7 
                 2.0 
                 55.3 
               
               
                 22B8 
                 6.000 
                 6.000 
                 6.000 
                 3.490 
                 1.270 
                 0.310 
                 0.200 
                 7.0 
                 4.5 
                 64.4 
               
               
                 24B3 
                 2.330 
                 1.580 
                 1.160 
                 0.790 
                 0.310 
                 0.130 
                 0.080 
                 1.0 
                 0.7 
                 68.6 
               
               
                 24C9 
                 3.670 
                 2.480 
                 2.140 
                 1.450 
                 0.540 
                 0.200 
                 0.110 
                 2.7 
                 1.5 
                 56.3 
               
               
                 27A3 
                 4.790 
                 4.720 
                 5.260 
                 3.930 
                 2.050 
                 0.960 
                 0.400 
                 20.5 
                 2.7 
                 13.0 
               
               
                 27D10 
                 4.090 
                 4.020 
                 3.950 
                 3.790 
                 1.870 
                 0.890 
                 0.410 
                 19.9 
                 3.7 
                 18.6 
               
               
                 29A3 
                 3.890 
                 4.060 
                 3.850 
                 3.320 
                 1.910 
                 0.570 
                 0.240 
                 13.6 
                 2.3 
                 17.1 
               
               
                 29H3 
                 4.520 
                 4.960 
                 4.290 
                 2.830 
                 1.420 
                 0.470 
                 0.210 
                 10.1 
                 1.3 
                 12.9 
               
               
                 29D4 
                 4.240 
                 5.570 
                 6.000 
                 4.090 
                 2.060 
                 0.660 
                 0.250 
                 16.3 
                 2.0 
                 12.5 
               
               
                 29F7 
                 4.420 
                 4.490 
                 4.260 
                 3.760 
                 1.680 
                 0.570 
                 0.220 
                 13.2 
                 1.1 
                 8.2 
               
               
                 29A11 
                 4.660 
                 6.000 
                 6.000 
                 5.090 
                 4.330 
                 2.160 
                 0.990 
                 63.5 
                 10.0 
                 15.7 
               
               
                 30A1 
                 4.520 
                 4.780 
                 6.000 
                 4.280 
                 3.370 
                 1.580 
                 1.110 
                 59.7 
                 29.4 
                 49.2 
               
               
                 30E3 
                 6.000 
                 4.780 
                 4.450 
                 2.120 
                 0.860 
                 0.320 
                 0.180 
                 5.7 
                 1.4 
                 23.9 
               
               
                 30F6 
                 3.040 
                 3.120 
                 2.420 
                 0.930 
                 0.350 
                 0.160 
                 0.090 
                 1.7 
                 0.9 
                 53.2 
               
               
                 30D7 
                 4.660 
                 5.090 
                 6.000 
                 4.240 
                 2.790 
                 1.020 
                 0.820 
                 36.2 
                 18.6 
                 51.3 
               
               
                 30H10 
                 4.420 
                 6.000 
                 6.000 
                 4.610 
                 2.520 
                 1.180 
                 0.640 
                 31.6 
                 10.6 
                 33.3 
               
               
                 31E11 
                 4.480 
                 6.000 
                 4.610 
                 3.600 
                 1.810 
                 0.620 
                 0.310 
                 13.8 
                 1.5 
                 10.6 
               
               
                 32B2 
                 6.000 
                 6.000 
                 6.000 
                 6.000 
                 3.330 
                 1.540 
                 0.800 
                 43.3 
                 10.9 
                 25.3 
               
               
                 32F4 
                 4.790 
                 5.560 
                 6.000 
                 4.610 
                 2.710 
                 0.970 
                 0.500 
                 26.5 
                 4.1 
                 15.4 
               
               
                 32G7 
                 2.030 
                 1.270 
                 0.960 
                 0.550 
                 0.380 
                 0.240 
                 0.110 
                 1.0 
                 0.8 
                 80.7 
               
               
                 32C11 
                 4.360 
                 6.000 
                 4.280 
                 2.020 
                 0.820 
                 0.270 
                 0.140 
                 4.6 
                 2.6 
                 55.4 
               
               
                 33B1 
                 2.710 
                 2.320 
                 1.620 
                 1.100 
                 0.550 
                 0.250 
                 0.200 
                 1.9 
                 1.5 
                 80.8 
               
               
                 33E1 
                 4.060 
                 4.240 
                 4.070 
                 3.510 
                 2.090 
                 0.980 
                 0.490 
                 23.8 
                 6.0 
                 25.2 
               
               
                 33C3 
                 4.960 
                 4.960 
                 4.310 
                 2.550 
                 0.840 
                 0.310 
                 0.180 
                 6.1 
                 2.0 
                 31.8 
               
               
                 33D5 
                 4.610 
                 4.560 
                 4.780 
                 6.000 
                 3.650 
                 1.700 
                 0.870 
                 52.1 
                 13.9 
                 26.6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Limited Antigen Coating Concentration ELISA Assay Results 
               
            
           
           
               
               
            
               
                   
                 Limited Antigen Coating 
               
               
                   
                 Concentration (ng/mL) 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 MAb ID 
                 50 
                 25 
                 12.5 
                 6.25 
                 3.125 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 1G6 
                 1.380 
                 0.650 
                 0.320 
                 0.200 
                 0.120 
               
               
                   
                 21A1 
                 2.650 
                 1.470 
                 0.810 
                 0.380 
                 0.230 
               
               
                   
                 21H6 
                 1.830 
                 0.970 
                 0.520 
                 0.230 
                 0.150 
               
               
                   
                 21H9 
                 2.280 
                 1.460 
                 0.820 
                 0.390 
                 0.220 
               
               
                   
                 22B8 
                 3.360 
                 1.840 
                 0.910 
                 0.420 
                 0.210 
               
               
                   
                 24B3 
                 0.380 
                 0.210 
                 0.120 
                 0.080 
                 0.070 
               
               
                   
                 24C9 
                 0.500 
                 0.230 
                 0.130 
                 0.090 
                 0.070 
               
               
                   
                 27A3 
                 2.300 
                 1.260 
                 0.540 
                 0.260 
                 0.150 
               
               
                   
                 27D10 
                 1.360 
                 0.540 
                 0.250 
                 0.140 
                 0.120 
               
               
                   
                 29A3 
                 2.270 
                 1.130 
                 0.490 
                 0.270 
                 0.160 
               
               
                   
                 29H3 
                 2.130 
                 1.030 
                 0.400 
                 0.240 
                 0.160 
               
               
                   
                 29D4 
                 3.310 
                 1.870 
                 0.800 
                 0.410 
                 0.230 
               
               
                   
                 29F7 
                 3.540 
                 2.190 
                 1.000 
                 0.510 
                 0.250 
               
               
                   
                 29A11 
                 3.430 
                 1.900 
                 1.050 
                 0.590 
                 0.300 
               
               
                   
                 30A1 
                 1.770 
                 0.870 
                 0.500 
                 0.270 
                 0.150 
               
               
                   
                 30E3 
                 2.710 
                 1.370 
                 0.730 
                 0.430 
                 0.240 
               
               
                   
                 30F6 
                 0.520 
                 0.310 
                 0.250 
                 0.130 
                 0.100 
               
               
                   
                 30D7 
                 2.930 
                 1.510 
                 0.790 
                 0.480 
                 0.270 
               
               
                   
                 30H10 
                 2.210 
                 1.180 
                 0.520 
                 0.310 
                 0.170 
               
               
                   
                 31E11 
                 2.370 
                 1.240 
                 0.680 
                 0.390 
                 0.200 
               
               
                   
                 32B2 
                 2.900 
                 1.690 
                 0.860 
                 0.420 
                 0.250 
               
               
                   
                 32F4 
                 2.220 
                 1.090 
                 0.740 
                 0.360 
                 0.230 
               
               
                   
                 32G7 
                 0.190 
                 0.150 
                 0.110 
                 0.080 
                 0.070 
               
               
                   
                 32C11 
                 2.480 
                 1.120 
                 0.690 
                 0.350 
                 0.200 
               
               
                   
                 33B1 
                 0.230 
                 0.200 
                 0.120 
                 0.080 
                 0.080 
               
               
                   
                 33E1 
                 0.850 
                 0.360 
                 0.150 
                 0.110 
                 0.070 
               
               
                   
                 33C3 
                 3.000 
                 1.320 
                 0.720 
                 0.370 
                 0.200 
               
               
                   
                 33D5 
                 3.810 
                 2.200 
                 1.320 
                 0.610 
                 0.330 
               
               
                   
                   
               
            
           
         
       
     
     Example 10 
     Structural Analysis of KDR Antibodies 
     The variable heavy chains and the variable light chains of the antibodies were sequenced to determine their DNA sequences. The complete sequence information for the anti-KDR antibodies is provided in the sequence listing with nucleotide and amino acid sequences for each gamma and kappa chain combination. The variable heavy sequences were analyzed to determine the VH family, the D-region sequence and the J-region sequence. The sequences were then translated to determine the primary amino acid sequence and compared to the germline VH, D and J-region sequences to assess somatic hypermutations. 
     Table 20 is a table comparing the antibody heavy chain regions to their cognate germ line heavy chain region. Table 21 is a table comparing the antibody kappa light chain regions to their cognate germ line light chain region. It should be appreciated that amino acid sequences among the sister clones collected from each hybridoma are identical. As an example, the heavy chain and light chain sequences for 30E3.3 would be identical to the sequences for 30E3.1 and 30E3.2. For ease of viewing Tables 20 and 21, sister clones are grouped together and identified by their common chain name. For example, the sequences for 30E3.1, 30E3.2 and 30E3.3 are listed as a common sequence under the chain name “30E3”. 
     The variable (V) regions of immunoglobulin chains are encoded by multiple germ line DNA segments, which are joined into functional variable regions (V H DJ H  or V K J K ) during B-cell ontogeny. The molecular and genetic diversity of the antibody response to KDR was studied in detail. These assays revealed several points specific to anti-KDR antibodies. It is of note that monoclonal antibodies 29A11, 32B2, 30E3, 30H10 and 32C11 appear to use D1-14 in the reverse complement orientation. 
     It should also be appreciated that where a particular antibody differs from its respective germline sequence at the amino acid level, the antibody sequence can be mutated back to the germline sequence. Such corrective mutations can occur at one, two, three or more positions, or a combination of any of the mutated positions, using standard molecular biological techniques. By way of non-limiting example, Table 16 shows that the light chain sequence of mAb 24B3 (SEQ ID NO.: 44) differs from the corresponding germline sequence (SEQ ID NO.:124) through a Ser to Asn mutation (mutation 1) in the CDR1 region, a Thr to Ala mutation (mutation 2) in the CDR2 region and an Arg to Ser mutation (mutation 3) in the CDR3 region. Thus, the amino acid or nucleotide sequence encoding the light chain of mAb 24B3 can be modified to change mutation 1 to yield the germline sequence at the site of mutation 1. Further, the amino acid or nucleotide sequence encoding the light chain of mAb 24B3 can be modified to change mutation 2 or mutation 3 to yield the germline sequence at the site of mutation 2 or mutation 3. Still further, the amino acid or nucleotide sequence encoding the light chain of mAb 24B3 can be modified to change both mutation 1 and mutation 2, or any other combination of two or more mutations to yield the germline sequence at those particular sites. Tables 15-19 below illustrate the positions of such variations from the germline for mAb 27D10, 24B3 and 33C3. Tables 15a-19a illustrate the positions of such variations from the germline for mAb 27D10, 24B3 and 33C3. Each row represents a unique combination of germline and non-germline residues at the position indicated by bold type. 
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 27D10 Heavy Chain (SEQ ID 
               
               
                 NO: 14) to Germline at the Indicated Residue Number 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 34 
                 39 
                 57 
                 69 
                 113 
                 114 
               
               
                   
                   
               
               
                   
                 R 
                 Y 
                 T 
                 Y 
                 L 
                 # 
               
               
                   
                 S 
                 D 
                 S 
                 F 
                 # 
                 Y 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 15a 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 27D10 Heavy Chain (SEQ ID NO: 
               
               
                 14) to Germline at the Indicated Residue Number 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 32 
                 34 
                 52 
                 60 
                 104 
                 105 
               
               
                   
                   
               
               
                   
                 R 
                 D 
                 T 
                 F 
                 L 
                 Y 
               
               
                   
                 S 
                 D 
                 T 
                 F 
                 L 
                 Y 
               
               
                   
                 R 
                 Y 
                 T 
                 F 
                 L 
                 Y 
               
               
                   
                 S 
                 Y 
                 T 
                 F 
                 L 
                 Y 
               
               
                   
                 R 
                 D 
                 S 
                 F 
                 L 
                 Y 
               
               
                   
                 S 
                 D 
                 S 
                 F 
                 L 
                 Y 
               
               
                   
                 R 
                 Y 
                 S 
                 F 
                 L 
                 Y 
               
               
                   
                 S 
                 Y 
                 S 
                 F 
                 L 
                 Y 
               
               
                   
                 R 
                 D 
                 T 
                 Y 
                 L 
                 Y 
               
               
                   
                 S 
                 D 
                 T 
                 Y 
                 L 
                 Y 
               
               
                   
                 R 
                 Y 
                 T 
                 Y 
                 L 
                 Y 
               
               
                   
                 S 
                 Y 
                 T 
                 Y 
                 L 
                 Y 
               
               
                   
                 R 
                 D 
                 S 
                 Y 
                 L 
                 Y 
               
               
                   
                 S 
                 D 
                 S 
                 Y 
                 L 
                 Y 
               
               
                   
                 R 
                 Y 
                 S 
                 Y 
                 L 
                 Y 
               
               
                   
                 S 
                 Y 
                 S 
                 Y 
                 L 
                 Y 
               
               
                   
                 R 
                 D 
                 T 
                 F 
                 — 
                 Y 
               
               
                   
                 S 
                 D 
                 T 
                 F 
                 — 
                 Y 
               
               
                   
                 R 
                 Y 
                 T 
                 F 
                 — 
                 Y 
               
               
                   
                 S 
                 Y 
                 T 
                 F 
                 — 
                 Y 
               
               
                   
                 R 
                 D 
                 S 
                 F 
                 — 
                 Y 
               
               
                   
                 S 
                 D 
                 S 
                 F 
                 — 
                 Y 
               
               
                   
                 R 
                 Y 
                 S 
                 F 
                 — 
                 Y 
               
               
                   
                 S 
                 Y 
                 S 
                 F 
                 — 
                 Y 
               
               
                   
                 R 
                 D 
                 T 
                 Y 
                 — 
                 Y 
               
               
                   
                 S 
                 D 
                 T 
                 Y 
                 — 
                 Y 
               
               
                   
                 R 
                 Y 
                 T 
                 Y 
                 — 
                 Y 
               
               
                   
                 S 
                 Y 
                 T 
                 Y 
                 — 
                 Y 
               
               
                   
                 R 
                 D 
                 S 
                 Y 
                 — 
                 Y 
               
               
                   
                 S 
                 D 
                 S 
                 Y 
                 — 
                 Y 
               
               
                   
                 R 
                 Y 
                 S 
                 Y 
                 — 
                 Y 
               
               
                   
                 S 
                 Y 
                 S 
                 Y 
                 — 
                 Y 
               
               
                   
                 R 
                 D 
                 T 
                 F 
                 L 
                 — 
               
               
                   
                 S 
                 D 
                 T 
                 F 
                 L 
                 — 
               
               
                   
                 R 
                 Y 
                 T 
                 F 
                 L 
                 — 
               
               
                   
                 S 
                 Y 
                 T 
                 F 
                 L 
                 — 
               
               
                   
                 R 
                 D 
                 S 
                 F 
                 L 
                 — 
               
               
                   
                 S 
                 D 
                 S 
                 F 
                 L 
                 — 
               
               
                   
                 R 
                 Y 
                 S 
                 F 
                 L 
                 — 
               
               
                   
                 S 
                 Y 
                 S 
                 F 
                 L 
                 — 
               
               
                   
                 R 
                 D 
                 T 
                 Y 
                 L 
                 — 
               
               
                   
                 S 
                 D 
                 T 
                 Y 
                 L 
                 — 
               
               
                   
                 R 
                 Y 
                 T 
                 Y 
                 L 
                 — 
               
               
                   
                 S 
                 Y 
                 T 
                 Y 
                 L 
                 — 
               
               
                   
                 R 
                 D 
                 S 
                 Y 
                 L 
                 — 
               
               
                   
                 S 
                 D 
                 S 
                 Y 
                 L 
                 — 
               
               
                   
                 R 
                 Y 
                 S 
                 Y 
                 L 
                 — 
               
               
                   
                 S 
                 Y 
                 S 
                 Y 
                 L 
                 — 
               
               
                   
                 R 
                 D 
                 T 
                 F 
                 — 
                 — 
               
               
                   
                 S 
                 D 
                 T 
                 F 
                 — 
                 — 
               
               
                   
                 R 
                 Y 
                 T 
                 F 
                 — 
                 — 
               
               
                   
                 S 
                 Y 
                 T 
                 F 
                 — 
                 — 
               
               
                   
                 R 
                 D 
                 S 
                 F 
                 — 
                 — 
               
               
                   
                 S 
                 D 
                 S 
                 F 
                 — 
                 — 
               
               
                   
                 R 
                 Y 
                 S 
                 F 
                 — 
                 — 
               
               
                   
                 S 
                 Y 
                 S 
                 F 
                 — 
                 — 
               
               
                   
                 R 
                 D 
                 T 
                 Y 
                 — 
                 — 
               
               
                   
                 S 
                 D 
                 T 
                 Y 
                 — 
                 — 
               
               
                   
                 R 
                 Y 
                 T 
                 Y 
                 — 
                 — 
               
               
                   
                 S 
                 Y 
                 T 
                 Y 
                 — 
                 — 
               
               
                   
                 R 
                 D 
                 S 
                 Y 
                 — 
                 — 
               
               
                   
                 S 
                 D 
                 S 
                 Y 
                 — 
                 — 
               
               
                   
                 R 
                 Y 
                 S 
                 Y 
                 — 
                 — 
               
               
                   
                 S 
                 Y 
                 S 
                 Y 
                 — 
                 — 
               
               
                   
                   
               
               
                   
                 “—” indicates the absence of a residue at that position with reference to SEQ ID NO: 14 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 24B3 Light Chain 
               
               
                 (SEQ ID NO: 44) to Germline at the 
               
               
                 Indicated Residue Number 
               
            
           
           
               
               
               
            
               
                 33 
                 67 
                 111 
               
               
                   
               
               
                 S 
                 T 
                 R 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 16a 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 24B3 Light Chain 
               
               
                 (SEQ ID NO: 44) to Germline at the 
               
               
                 Indicated Residue Number 
               
            
           
           
               
               
               
            
               
                 31 
                 51 
                 93 
               
               
                   
               
               
                 S 
                 T 
                 R 
               
               
                 N 
                 T 
                 R 
               
               
                 S 
                 A 
                 R 
               
               
                 N 
                 A 
                 R 
               
               
                 S 
                 T 
                 S 
               
               
                 N 
                 T 
                 S 
               
               
                 S 
                 A 
                 S 
               
               
                 N 
                 A 
                 S 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 24B3 Heavy Chain (SEQ ID NO: 42) 
               
               
                 to Germline at the Indicated Residue Number 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 33 
                 57 
                 60 
                 61 
                 107 
                 108 
                 109 
                 110 
                 113 
               
               
                   
               
               
                 S 
                 F 
                 S 
                 S 
                 A 
                 R 
                 D 
                 # 
                 G 
               
               
                 S 
                 S 
                 G 
                 S 
                 A 
                 R 
                 # 
                 D 
                 G 
               
               
                 T 
                 S 
                 S 
                 R 
                 A 
                 R 
                 # 
                 # 
                 E 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 17a 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 24B3 Heavy Chain (SEQ ID NO: 
               
               
                 42) to Germline at the Indicated Residue Number 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 31 
                 50 
                 53 
                 54 
                 97 
                 98 
                 99 
                 100 
                 103 
               
               
                   
               
               
                 T 
                 F 
                 G 
                 R 
                 S 
                 K 
                 D 
                 D 
                 E 
               
               
                 S 
                 F 
                 G 
                 R 
                 S 
                 K 
                 D 
                 D 
                 E 
               
               
                 T 
                 S 
                 G 
                 R 
                 S 
                 K 
                 D 
                 D 
                 E 
               
               
                 S 
                 S 
                 G 
                 R 
                 S 
                 K 
                 D 
                 D 
                 E 
               
               
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                 F 
                 S 
                 R 
                 S 
                 K 
                 D 
                 D 
                 E 
               
               
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                 F 
                 S 
                 R 
                 S 
                 K 
                 D 
                 D 
                 E 
               
               
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                 S 
                 S 
                 R 
                 S 
                 K 
                 D 
                 D 
                 E 
               
               
                 S 
                 S 
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                 K 
                 D 
                 D 
                 E 
               
               
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                 F 
                 G 
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                 K 
                 D 
                 D 
                 E 
               
               
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                 K 
                 D 
                 D 
                 E 
               
               
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                 S 
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                 D 
                 D 
                 E 
               
               
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                 D 
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                 S 
                 R 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 S 
                 S 
                 R 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 F 
                 G 
                 S 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 F 
                 G 
                 S 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 S 
                 G 
                 S 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 S 
                 G 
                 S 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 F 
                 S 
                 S 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 F 
                 S 
                 S 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 S 
                 S 
                 S 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 S 
                 S 
                 S 
                 S 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 F 
                 G 
                 R 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 F 
                 G 
                 R 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 S 
                 G 
                 R 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 S 
                 G 
                 R 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 F 
                 S 
                 R 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 F 
                 S 
                 R 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 S 
                 S 
                 R 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 S 
                 S 
                 R 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 F 
                 G 
                 S 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 F 
                 G 
                 S 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 S 
                 G 
                 S 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 S 
                 G 
                 S 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 F 
                 S 
                 S 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 F 
                 S 
                 S 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 T 
                 S 
                 S 
                 S 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                 S 
                 S 
                 S 
                 S 
                 A 
                 R 
                 — 
                 — 
                 G 
               
               
                   
               
               
                 “—” indicates the absence of a residue at that position with reference to SEQ ID NO: 42 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 33C3 Light Chain (SEQ ID NO: 
               
               
                 76) to Germline at the Indicated Residue Number 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 7 
                 9 
                 40 
                 45 
                 58 
                 67 
                 109 
                 112 
                 144 
               
               
                   
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 18a 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 33C3 Light Chain (SEQ ID NO: 
               
               
                 76) to Germline at the Indicated Residue Number 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 7 
                 9 
                 32 
                 37 
                 50 
                 51 
                 91 
                 94 
                 103 
               
               
                   
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 T 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 A 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 A 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 A 
                 T 
                 T 
                 N 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 A 
                 T 
                 T 
                 N 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 T 
                 N 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 T 
                 N 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 T 
                 N 
               
               
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                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 I 
                 K 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 T 
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                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
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                 Q 
                 G 
                 A 
                 T 
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                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
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                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 T 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 Q 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 T 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 H 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 H 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 H 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 H 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 Q 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 G 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 H 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 H 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 H 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 H 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 H 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 F 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 L 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 Y 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                 S 
                 S 
                 Y 
                 Q 
                 A 
                 A 
                 S 
                 T 
                 K 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 19 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 33C3 Heavy Chain 
               
               
                 (SEQ ID NO: 74) to Germline at the Indicated 
               
               
                 Residue Number 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 17 
                 60 
                 61 
                 98 
                 105 
                 109 
                 117 
                 143 
               
               
                   
                   
               
               
                   
                 G 
                 H 
                 S 
                 A 
                 Y 
                 D 
                 # 
                 T 
               
               
                   
                 G 
                 S 
                 G 
                 A 
                 Y 
                 # 
                 F 
                 T 
               
               
                   
                 E 
                 S 
                 S 
                 A 
                 Y 
                 # 
                 # 
                 T 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 19a 
               
             
            
               
                   
               
               
                 Exemplary Mutations of mAB 33C3 Heavy Chain 
               
               
                 (SEQ ID NO: 74) to Germline at the Indicated 
               
               
                 Residue Number 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 16 
                 53 
                 54 
                 88 
                 95 
                 99 
                 107 
                 117 
               
               
                   
                   
               
               
                   
                 E 
                 H 
                 G 
                 V 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 G 
                 V 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 G 
                 V 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 G 
                 V 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 S 
                 V 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 S 
                 V 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 S 
                 V 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 S 
                 V 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 G 
                 A 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 G 
                 A 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 G 
                 A 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 G 
                 A 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 S 
                 A 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 S 
                 A 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 S 
                 A 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 S 
                 A 
                 F 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 G 
                 V 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 G 
                 V 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 G 
                 V 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 G 
                 V 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 S 
                 V 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 S 
                 V 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 S 
                 V 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 S 
                 V 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 G 
                 A 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 G 
                 A 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 G 
                 A 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 G 
                 A 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 S 
                 A 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 S 
                 A 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 S 
                 A 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 S 
                 A 
                 Y 
                 D 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 G 
                 V 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 G 
                 V 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 G 
                 V 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 G 
                 V 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 S 
                 V 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 S 
                 V 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 S 
                 V 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 S 
                 V 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 G 
                 A 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 G 
                 A 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 G 
                 A 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 G 
                 A 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 S 
                 A 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 S 
                 A 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 S 
                 A 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 S 
                 A 
                 F 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 G 
                 V 
                 Y 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 G 
                 V 
                 Y 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 G 
                 V 
                 Y 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 S 
                 G 
                 V 
                 Y 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 H 
                 S 
                 V 
                 Y 
                 — 
                 F 
                 A 
               
               
                   
                 G 
                 H 
                 S 
                 V 
                 Y 
                 — 
                 F 
                 A 
               
               
                   
                 E 
                 S 
                 S 
                 V 
                 Y 
                 — 
                 F 
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                 V 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 S 
                 S 
                 V 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 S 
                 S 
                 V 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 H 
                 G 
                 A 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 H 
                 G 
                 A 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 S 
                 G 
                 A 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 S 
                 G 
                 A 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 H 
                 S 
                 A 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 H 
                 S 
                 A 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 S 
                 S 
                 A 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 S 
                 S 
                 A 
                 F 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 H 
                 G 
                 V 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 H 
                 G 
                 V 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 S 
                 G 
                 V 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 S 
                 G 
                 V 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 H 
                 S 
                 V 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 H 
                 S 
                 V 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 S 
                 S 
                 V 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 S 
                 S 
                 V 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 H 
                 G 
                 A 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 H 
                 G 
                 A 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 S 
                 G 
                 A 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 S 
                 G 
                 A 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 H 
                 S 
                 A 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 H 
                 S 
                 A 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 E 
                 S 
                 S 
                 A 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                 G 
                 S 
                 S 
                 A 
                 Y 
                 — 
                 — 
                 T 
               
               
                   
                   
               
               
                   
                 “—” indicates the absence of a residue at that position with reference to SEQ ID NO: 74 
               
            
           
         
       
     
     The skilled person will be aware that there are alternative methods of defining CDR boundaries. The starting residue of VH CDR1 in the Table 20a has been defined according to the method as described in Scaviner D, Barbie V, Ruiz M, Lefranc M-P. Protein Displays of the Human Immunoglobulin Heavy, Kappa and Lambda Variable and Joining Regions. Exp Clin Immunogenet 1999, 16:234-240. The remaining CDR boundaries in Table 20a and Table 21a are defined according to the Kabat definition. 
     All CDR boundaries in Table 20b and Table 21b are defined according to the Kabat definition. 
     
       
         
           
               
             
               
                 TABLE 20a 
               
             
            
               
                   
               
               
                 Heavy chain analysis 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 Chain 
                 SEQ 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 Name 
                 ID NO: 
                 V 
                 D 
                 J 
                 FR1 
                 CDR1 
                 FR2 
                 CDR2 
                 FR3 
                 CDR3 
                 FR4 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 109  
                 Germline 
                 QVQLVQSGAEVKK 
                 GYTFTSY 
                 WVRQATG 
                 WMNPNSG 
                 RVTMTRNTSIST 
                 ##YS# 
                 WGQGT 
               
               
                   
                   
                   
                 PGASVKVSCKAS 
                 DIN 
                 QGLEWMG 
                 NTGYAQK 
                 AYMELSSLRSED 
                 ##WFD 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                 PCG 
                 TAVYYCAR 
                 P 
                 S 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 33D5 
                 58 
                 VH1-8 
                 D5-12 
                 JH5B 
                 QVQLVQSGAEVKK 
                 GYTFGSY 
                 WVRQATG 
                 WMNPNSG 
                 RVTLTRNTSIRT 
                 GGYSN 
                 WGQGS 
               
               
                   
                   
                   
                   
                   
                 PGASVKVSCKAS 
                 DIN 
                 QGLEWMG 
                 NTGYAQK 
                 VYMELSSLRSED 
                 LGWFD 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 FCG 
                 TAVYYCAR 
                 P 
                 S 
               
               
                   
               
               
                 29H3 
                 54 
                 VH1-8 
                 D6-13 
                 JH5B 
                 QVQLVQSGAEVKK 
                 GYTFTSY 
                 WVRQATG 
                 WMNPNSG 
                 RVTMTRNTSINT 
                 GGYSN 
                 WGQGT 
               
               
                   
                   
                   
                   
                   
                 PGASVKVSCKAS 
                 DIN 
                 QGLEWMG 
                 KTGYAQK 
                 AYMELSSLRSED 
                 LGWFD 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 FCG 
                 TAVYYCAR 
                 P 
                 S 
               
               
                   
               
               
                 29F7 
                 62 
                 VH1-8 
                 D6-13 
                 JH5B 
                 QVQLVQSGAEVKK 
                 GYTFTSY 
                 WVRQATG 
                 WMNPNSG 
                 RVTMTRSTSIST 
                 GGYSN 
                 WGQGT 
               
               
                   
                   
                   
                   
                   
                 PGASVKVSCKAS 
                 DIN 
                 QGLEWMG 
                 KTGYAQK 
                 AYMELSSLRSED 
                 LGWFD 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 FCG 
                 TAVYYCAR 
                 P 
                 S 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 110  
                 Germline 
                 QVQLVESGGGLVK 
                 GFTFSDY 
                 WIRQAPG 
                 YISSSGS 
                 RFTISRDNAKNS 
                 DDFWS 
                 WGQGT 
               
               
                   
                   
                   
                 PGGSLRLSCAAS 
                 YMS 
                 KGLEWVS 
                 TIYYADS 
                 LYLQMNSLRAED 
                 GY##N 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                 VKG 
                 TAVYYCAR 
                 WFDP 
                 S 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 33C3 
                 74 
                 VH3-11 
                 D3-3 
                 JH5B 
                 QVQKVESGGGKVK 
                 GFTFSDY 
                 WIRQAPG 
                 YISHGGS 
                 RFTISRDNAKNS 
                 DDFWS 
                 WGQGWA 
               
               
                   
                   
                   
                   
                   
                 PGESLRLSCAAS 
                 YMS 
                 KGLEWVS 
                 TIYYADS 
                 LYLQMNSLRVED 
                 GYYFN 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 VKG 
                 TAVYFCAR 
                 WFDP 
                 S 
               
               
                   
               
               
                 31E11 
                 70 
                 VH3-11 
                 D3-3 
                 JH5B 
                 QVQLVESGGGLVK 
                 GFTFSDY 
                 WIRQAPG 
                 YISISGS 
                 RFTISRDNAKNS 
                 DDFWS 
                 WGQGT 
               
               
                   
                   
                   
                   
                   
                 PGGSLRLSCAAS 
                 YMS 
                 KGLEWVS 
                 TIYYADS 
                 LYLQMNSLRAED 
                 GYYFN 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 VKG 
                 TAVYYCAR 
                 WFDP 
                 S 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 111  
                 Germline 
                 QVQLVESGGGLVK 
                 GFTFSDY 
                 WIRQAPG 
                 YISSSGS 
                 RFTISRDNAKNS 
                 ##YS# 
                 WGQGT 
               
               
                   
                   
                   
                 PGGSLRLSCAAS 
                 YMS 
                 KGLEWVS 
                 TIYYADS 
                 LYLQMSLRAED 
                 SGWY# 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                 VKG 
                 TAVYYCAR 
                 #FDY 
                 S 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 21A1 
                 66 
                 VH3-11 
                 D6-19 
                 JH4B 
                 QVQLVESGGGLVK 
                 GFTFSDY 
                 WIRQAPG 
                 YISNSGI 
                 RFTISRDNAKNS 
                 EEWYS 
                 WGQGT 
               
               
                   
                   
                   
                   
                   
                 PGGSLRLSCAAS 
                 YMS 
                 KGLEWVS 
                 TIYYADS 
                 LYLQMNSLRAGD 
                 SSWYR 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 VKG 
                 TAVYYCAR 
                 NFDY 
                 S 
               
               
                   
               
               
                 21H6 
                 66 
                 VH3-11 
                 D6-19 
                 JH4B 
                 QVQLVESGGGLVK 
                 GFTFSDY 
                 WIRAQPG 
                 YISNSGI 
                 RFTISRDNAKNS 
                 EEWYS 
                 WGQGT 
               
               
                   
                   
                   
                   
                   
                 PGGSLRLSCAAS 
                 YMS 
                 KGLEWVS 
                 TIYYADS 
                 LYLQMNSLRAGD 
                 SSWYR 
                 LVTVS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 VKG 
                 TAVYYCAR 
                 NFDY 
                 S 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 112  
                 Germline 
                 EVQLVESGGGL 
                 GFTFSSY 
                 WVRQAPG 
                 SISSSSS 
                 RFTISRDNAKNSL 
                 ##WF### 
                 WGQGTLV 
               
               
                   
                   
                   
                 VKPGGSLRLSC 
                 SMN 
                 KGLEWVS 
                 YIYYADS 
                 YLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYC## 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 24C9 
                 34 
                 VH3-21 
                 D3-10 
                 JH4B 
                 EVQLVESGGGL 
                 GFTFSTY 
                 WVRQAPG 
                 FISGRSS 
                 RFTISRDNAKNSL 
                 DDWFEEL 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPGGSLRLSC 
                 SMN 
                 KGLEWVS 
                 YIYYADS 
                 YLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCSK 
                   
                   
               
               
                   
               
               
                 32G7 
                 38 
                 VH3-21 
                 D3-10 
                 JH4B 
                 EVQLVESGGGL 
                 GFTISSY 
                 WVRQAPG 
                 FISSRSN 
                 RFTISRDNAKNSL 
                 DDWFEEL 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPGGSLRLSC 
                 SMN 
                 KGLEWVS 
                 YIYYADS 
                 YLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCSK 
                   
                   
               
               
                   
               
               
                 24B3 
                 42 
                 VH3-21 
                 D3-10 
                 JH4B 
                 EVQLVESGGGL 
                 GFTFSTY 
                 WVRQAPG 
                 FISGRSS 
                 RFTISRDNAKNSL 
                 DDWFEEL 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPGGSLRLSC 
                 SMN 
                 KGLEWVS 
                 YIYYADS 
                 YLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCSK 
                   
                   
               
               
                   
               
               
                 33B1 
                 46 
                 VH3-21 
                 D3-10 
                 JH4B 
                 EVQLVESGGGL 
                 GFTFSSY 
                 WVRQAPG 
                 FIDSRSS 
                 RFTISRDNAKNSL 
                 DDWFEEL 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPGGSLRLSC 
                 TMN 
                 KGLEWVS 
                 YIYYADS 
                 YLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCSK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 113  
                 Germline 
                 EVQLLESGGGL 
                 GFTFSSY 
                 WVRQAPG 
                 AISGSGG 
                 RFTISRDNSKNTL 
                 ##### 
                 WGQGTLV 
               
               
                   
                   
                   
                 VQPGGSLRLSC 
                 AMS 
                 KGLEWVS 
                 STYYADS 
                 YLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29A11 
                 90 
                 VH3-23 
                   
                 JH4B 
                 EVQLLESGGGL 
                 GFTFSNY 
                 WVRQAPG 
                 AISGGGS 
                 RFTISRDNSKNTL 
                 ASGDD 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VQPGGSLRLSC 
                 AMN 
                 KGLEWVS 
                 NTYYADS 
                 YLQMNRLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAK 
                   
                   
               
               
                   
               
               
                 30H10 
                 94 
                 VH3-23 
                   
                 JH4B 
                 EVQLLESGGGL 
                 GFTFSNY 
                 WVRQAPG 
                 AISGSGG 
                 RFTISRDNSKNTL 
                 ASGDD 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VQPGGSLRLSC 
                 AMS 
                 KGLEWVS 
                 ITYYADS 
                 FLQMNSLRAEDTA 
                   
                 SVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAK 
                   
                   
               
               
                   
               
               
                 32B2 
                 82 
                 VH3-23 
                   
                 JH4B 
                 EVQLLESGGGL 
                 GFTFSNY 
                 WVRQAPG 
                 AISGRGG 
                 RFTISRDNSKNTL 
                 ASGDD 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VQPGGSLRLSC 
                 AMS 
                 KGLDWVS 
                 STYYADS 
                 FLLMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAK 
                   
                   
               
               
                   
               
               
                 32C11 
                 98 
                 VH3-23 
                   
                 JH4B 
                 EVQLLESGGTL 
                 GFTFSNY 
                 WVRQAPG 
                 AISGRAG 
                 RFTISRDNSKNTL 
                 ASGDD 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VQPGGSLRLSC 
                 AMS 
                 KGLEWVS 
                 STYYADS 
                 FLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAK 
                   
                   
               
               
                   
               
               
                 30E3 
                 86 
                 VH3-23 
                   
                 JH4B 
                 EVQLLESGGGL 
                 GFTFSNY 
                 WVRQAPG 
                 AISGSGR 
                 RFTLSRDNSKNTL 
                 ASGDD 
                 WGWGTLV 
               
               
                   
                   
                   
                   
                   
                 VQPGGSLRLSC 
                 ALS 
                 KGLEWVS 
                 NTYYADS 
                 FLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 114  
                 Germline 
                 EVQLLESGGGL 
                 GFTFSSY 
                 WVRQAPG 
                 AISGSGG 
                 RFTISRDNSKNTL 
                 VATDY 
                 WGQGTLV 
               
               
                   
                   
                   
                 VQPGGSLRLSC 
                 AMS 
                 KGLEWVS 
                 STYYADS 
                 YLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 1G6 
                 106  
                 VH3-23 
                 D5-12 
                 JH4B 
                 EVQLLESGGGL 
                 GFTFSSY 
                 WVRQAPG 
                 AISGGGG 
                 RFTISRDNSKNTL 
                 VATDY 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VQPGGSLRLSC 
                 AMS 
                 KGLEWVS 
                 NTYYADS 
                 YLQMNSLRAEDTA 
                   
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCSK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 115  
                 Germline 
                 QVQLVESGGGV 
                 GFTFSSY 
                 WVRQAPG 
                 VIWYGDS 
                 RFTISRDNSKNTL 
                 ##WN##Y 
                 WGQGTTV 
               
               
                   
                   
                   
                 VQPGRSLRLSC 
                 GMH 
                 KGLEWVA 
                 NKYYADS 
                 TLQMNSLRAEDTA 
                 YYGMDV 
                 TVSS 
               
               
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCA# 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 30F6 
                 78 
                 VH3-33 
                 D1-20 
                 JHB6 
                 QVQLVESGGGV 
                 GFTFSTY 
                 WVRQAPG 
                 VIWYDGS 
                 RFTISRDNSKNTL 
                 SYWNDDY 
                 WGQGTTV 
               
               
                   
                   
                   
                   
                   
                 VQPGRSLRLSC 
                 GMH 
                 KGLEWVA 
                 NKYYADS 
                 YLQMNSLRAEDTA 
                 YYGMDV 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 116  
                 Germline 
                 QVQLVESGGGV 
                 GFTFSSY 
                 WVRQAPG 
                 VIWYDGS 
                 RFTISRDNSKNTL 
                 ##TMVRG 
                 WGQGTTV 
               
               
                   
                   
                   
                 VQPGRSLRLSC 
                 GMH 
                 KGLEWVA 
                 NKYYADS 
                 YLQMNSLRAEDTA 
                 VII##YY 
                 TVSS 
               
               
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAR 
                 YGMDV 
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 30D7 
                  2 
                 VH3-33 
                 D3-10 
                 JHB6 
                 QVPLVESGGGV 
                 GFTFSSY 
                 WVRQAPG 
                 VIWYDGS 
                 RFTISRDNSKNTL 
                 GVTMVRG 
                 WGQGTTV 
               
               
                   
                   
                   
                   
                   
                 VQPGRSLRLSC 
                 GMH 
                 KGLEWVA 
                 NKYYADS 
                 YLQMNSLRAEDTA 
                 VIIARYY 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VKG 
                 VYYCAR 
                 YGLDV 
                   
               
               
                   
               
               
                 21H9 
                  6 
                 VH3-33 
                 D3-10 
                 JHB6 
                 QVPLVESGGGV 
                 GFTFSSY 
                 WVRQAPG 
                 VIWYDGS 
                 RFTISRDNSKNTL 
                 GVTMVRG 
                 GWQGTTV 
               
               
                   
                   
                   
                   
                   
                 VQPGRSLRLSC 
                 GMH 
                 KGLEWVA 
                 NKYYADS 
                 YLQMNSLRAEDTA 
                 LIIARYY 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 AAS 
                   
                   
                 VQG 
                 VYYCAR 
                 YGLDV 
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 117  
                 Germline 
                 QVQLQESGPGL 
                 GGSISSY 
                 WIRQPAG 
                 RIYTSGS 
                 RVTMSVDTSKNQF 
                 ###WNY# 
                 WGQGTLV 
               
               
                   
                   
                   
                 VKPSETLSLTC 
                 YWS 
                 KGLEWIG 
                 TNYNPSL 
                 SLKLSSVTAADTA 
                 ##FDY 
                 TVSS 
               
               
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29A3 
                 102  
                 VH4-4 
                 D1-7 
                 JH4B 
                 QVQLQESGPGL 
                 GGSISSY 
                 WIRQPAG 
                 RIYFSGR 
                 RVTMSVETSKNQF 
                 DGGWNYD 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPSETLSLTC 
                 FWS 
                 KGLEWIG 
                 TNYNPSL 
                 SLKLNSVTAADTA 
                 VAFDY 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 118  
                 Germline 
                 QVQLQESGPGL 
                 GGSISSG 
                 WIRQHPG 
                 YIYYSGS 
                 RVTISVDTSKNQF 
                 ##SSSW# 
                 WGRGTLV 
               
               
                   
                   
                   
                 VKPSQTLSLTC 
                 GYYWS 
                 KLEWIG 
                 TYYNPSL 
                 SLKLSSVTAADTA 
                 #WYFDL 
                 TVSS 
               
               
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCA# 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 33E1 
                 50 
                 VH4-31 
                 D6-13 
                 JH2 
                 QVQLQESGPGL 
                 GGSISSG 
                 WIRQHPG 
                 HIYYSGS 
                 RVTISVDTSKNHF 
                 TNSSSWS 
                 WGRGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPSQTLSLTC 
                 DHYWS 
                 KLEWIG 
                 TDYNPSL 
                 SLKLNSVTAADTA 
                 DWYFDL 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 119  
                 Germline 
                 QLQLQESGPGL 
                 GGSISSS 
                 WIRQPPG 
                 SIYYSGS 
                 RVTISVDTSKNQF 
                 ##WLV##Y 
                 WGQGTLV 
               
               
                   
                   
                   
                 VKPSETLSLTC 
                 SYYWG 
                 KLEWIG 
                 TYYNPSL 
                 SLKLSSVTAADTA 
                 FDY 
                 TVSS 
               
               
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCA# 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 22B8 
                 30 
                 VH4-39 
                 D6-13 
                 JH4B 
                 QLQLQESGPGL 
                 GGSISSR 
                 WVRQPPG 
                 TIYYSGH 
                 RVTISVDTSKNQF 
                 QQLVLYY 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPSETLSLTC 
                 YYYWG 
                 KGLEWIG 
                 TYYNPSL 
                 SLKLISVTAADTA 
                 FDF 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 TVS 
                   
                   
                 KT 
                 VYYCAT 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 120  
                 Germline 
                 QLQLQESGPGL 
                 GGSISSS 
                 WIRQPPG 
                 SIYYSGS 
                 RVTISVDTSKNQF 
                 #QLV##Y 
                 WGQGTLV 
               
               
                   
                   
                   
                 VKPSETLSLTC 
                 SYYWG 
                 KGLEWIG 
                 TYYNPSL 
                 SLKLSSVTAADTA 
                 FDY 
                 TVSS 
               
               
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 27A3 
                 10 
                 VH4-39 
                 D6-6 
                 JH4B 
                 QLQLQESGPGL 
                 GGSISSR 
                 WIRQPPG 
                 TIYYSGD 
                 RVTISVDTSKNQF 
                 QQLVLYY 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPSETLSLTC 
                 SDYWG 
                 KGLEWIG 
                 TYYNPSL 
                 SLKLSSVTAADTA 
                 FDY 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCAR 
                   
                   
               
               
                   
               
               
                 27D10 
                 14 
                 VH4-39 
                 D6-6 
                 JH4B 
                 QLQLQESGPGL 
                 GGSISSR 
                 WIRQPPG 
                 TYYSGS 
                 RVTISVDTSKNQF 
                 QQLVLYY 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPSETLSLTC 
                 SDYWG 
                 KGLEWIG 
                 TFYNPSL 
                 SLKLSSVTAADTA 
                 FDY 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCAR 
                   
                   
               
               
                   
               
               
                 30A1 
                 26 
                 VH4-39 
                 D6-6 
                 JH4B 
                 QLQLQESGPGL 
                 GGSISSP 
                 WIRQPPG 
                 TIYYSGN 
                 RVTISVDTSKNQF 
                 QQLVLYY 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPSETLSLTC 
                 NYYWG 
                 KGLEWIG 
                 TFYNPSL 
                 SLKLSSVTAADTA 
                 FDF 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCAT 
                   
                   
               
               
                   
               
               
                 32F4 
                 18 
                 VH4-39 
                 D6-6 
                 JH4B 
                 QLQLQESGPGL 
                 GGSISSR 
                 WIRQPPG 
                 TIYYSGN 
                 RVTISVDTSKKQF 
                 QQLVLYY 
                 WGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPSGTLSLSC 
                 SDYWG 
                 KGLEWIG 
                 TFYNPSL 
                 SLKLSSVTAADTA 
                 FDY 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 VYYCAR 
                   
                   
               
               
                   
               
               
                 29D4 
                 22 
                 VH4-39 
                 D6-6 
                 JH4B 
                 QLQLQESGPGL 
                 GGSISSR 
                 WIRQPPG 
                 TIYYSGH 
                 RVSISVDTSKNQF 
                 QQLVLYY 
                 QGQGTLV 
               
               
                   
                   
                   
                   
                   
                 VKPSETLSLTC 
                 SNYWG 
                 KGLEWIG 
                 TYYNPSL 
                 SLKLSSVTATDTA 
                 FEY 
                 TVSS 
               
               
                   
                   
                   
                   
                   
                 TVS 
                   
                   
                 KS 
                 LYYCAR 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 20b 
               
             
            
               
                   
               
               
                 Heavy chain analysis 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 Chain 
                 SEQ 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 Name 
                 ID NO: 
                 V 
                 D 
                 J 
                 FR1 
                 CDR1 
                 FR2 
                 CDR2 
                 FR3 
                 CDR3 
                 FR4 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 132  
                 Germline 
                 QVQLQESGPGLVKPS 
                 SGGYY 
                 WSWIRQHP 
                 YIYYSGST 
                 RVTISVDTSKN 
                 --SSSW-- 
                 WGRGTLVT 
               
               
                   
                   
                   
                 QTLSLTCTVSGGSIS 
                   
                 GKGLEWIG 
                 YYNPSLKS 
                 QFSLKLSSVTA 
                 WYFDL 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 33E1 
                 50 
                 VH4-31 
                 D6-13 
                 JH2 
                 QVQLQESGPGLVKPS 
                 SGDHY 
                 WSWIRQHP 
                 HIYYSGST 
                 RVTSIDVTSKN 
                 TNSSSWSDWY 
                 WGRGTLVT 
               
               
                   
                   
                   
                   
                   
                 QTLSLTCTVSGGSIS 
                   
                 GKGLEWIG 
                 DYNPSLKS 
                 HFSLKLNSVTA 
                 FDL 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 133  
                 Germline 
                 EVQLLESGGGLVQPG 
                 SYAMS 
                 WVRQAPGK 
                 AISGSGGS 
                 RFTISRDNSKN 
                 GSGDY 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GLEWVS 
                 TYYADSVK 
                 TLYLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29A11 
                 90 
                 VH3-23 
                 D1-14RC 
                 JH4 
                 EVQLLESGGGLVQPG 
                 NYAMN 
                 WVRQAPGK 
                 AISGGGGN 
                 RFTISRDNSKN 
                 ASGDY 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 TYYADSVK 
                 TLYLQMNRLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAK 
                   
                   
               
               
                   
               
               
                 32B2 
                 82 
                 VH3-23 
                 D1-14RC 
                 JH4 
                 EVQLLESGGGLVQPG 
                 NYAMS 
                 WVRQAPGK 
                 AISGRGGS 
                 RFTISRDNSKN 
                 ASGDD 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLDWVS 
                 TYYADSVK 
                 TLFLLMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAK 
                   
                   
               
               
                   
               
               
                 30E3 
                 86 
                 VH3-23 
                 D1-14RC 
                 JH4 
                 EVQLLESGGGLVQPG 
                 NYALS 
                 WVRQAPGK 
                 AISGSGRN 
                 RFTLSRDNSKN 
                 ASGDN 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 TYYADSVK 
                 TLFLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAK 
                   
                   
               
               
                   
               
               
                 30H10 
                 94 
                 VH3-23 
                 D1-14RC 
                 JH4 
                 EVQLLESGGGLVQPG 
                 NYAMS 
                 WVRQAPGK 
                 AISGSGGI 
                 RFTISRDNSKN 
                 ASGDN 
                 WGQGTLVS 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 TYYADSVK 
                 TLFLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAK 
                   
                   
               
               
                   
               
               
                 32C11 
                 98 
                 VH3-23 
                 D1-14RC 
                 JH4 
                 EVQLLESGGTLVQPG 
                 NYAMS 
                 WVRQAPGK 
                 AISGRAGS 
                 RFTISRDNSKN 
                 ASGDD 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 TYYADSVK 
                 TLFLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 134  
                 Germline 
                 QLQLQESGPGLVKPS 
                 SSSYYWG 
                 WIRQPPGK 
                 SIYYSGST 
                 RVTISVDTSKN 
                 QQLV--YFDY 
                 WGQGTLVT 
               
               
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 YYNPSLKS 
                 QFSLKLSSVTA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 22B8 
                 30 
                 VH4-39 
                 D6-13 
                 JH4 
                 QLQLQESGPGLVKPS 
                 SRYYYWG 
                 WVRQPPGK 
                 TIYYSGHT 
                 RVTISVDTSKN 
                 QQLVLYYFDF 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 YYNPSLKT 
                 QFSLKLISVTA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAT 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 135  
                 Germline 
                 QLQLQESGPGLVKPS 
                 SSSYYWG 
                 WIRQPPGK 
                 SIYYSGST 
                 RVTISVDTSKN 
                 QQLV--YFDY 
                 WGQGTLVT 
               
               
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 YYNPSLKS 
                 QFSLKLSSVTA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 30A1 
                 26 
                 VH4-39 
                 D6-13 
                 JH4 
                 QLQLQESGPGLVKPS 
                 SPNYYWG 
                 WIRQPPGK 
                 TIYYSGNT 
                 RVTISVDTSKN 
                 QQLVLYYFDF 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 FYNPSLKS 
                 QFSLKLSSVTA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAT 
                   
                   
               
               
                   
               
               
                 27A3 
                 10 
                 VH4-39 
                 D6-13 
                 JH4 
                 QLQLQESGPGLVKPS 
                 SRSDYWG 
                 WIRQPPGK 
                 TIYYSGDT 
                 RVTISVDTSKN 
                 QQLVLYYFDY 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 YYNPSLKS 
                 QFSLKLSSVTA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
               
                 27D10 
                 14 
                 VH4-39 
                 D6-13 
                 JH4 
                 QLQLQESGPGLVKPS 
                 SRSDYWG 
                 WIRQPPGK 
                 TIYYSGST 
                 RVTISVDTSKN 
                 QQLVLYYFDY 
                 QGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 RYNPSLKS 
                 QFSLKLSSVTA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
               
                 32F4 
                 18 
                 VH4-39 
                 D6-13 
                 JH4 
                 QLQLQESGPGLVKPS 
                 SRSDYWG 
                 WIRQPPGK 
                 TIYYSGNT 
                 RVTISVDTSKK 
                 QQLVLYYFDY 
                 QGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GTLSLSCTVSGGSIS 
                   
                 GLEWIG 
                 FYNPSLKS 
                 QFSLKLSSVTA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
               
                 29D4 
                 22 
                 VH4-39 
                 D6-13 
                 JH4 
                 QLQLQESGPGLVKPS 
                 SRSNYWG 
                 WIRQPPGK 
                 TIYYSGHT 
                 RVSISVDTSKN 
                 QQLVLYYFEY 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 YYNPSLKS 
                 QFSLKLSSVTA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 TDTALYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 136  
                 Germline 
                 EVQLVESGGGLVKPG 
                 SYSMN 
                 WVRQAPGK 
                 SISSSSSY 
                 RFTISRDNAKN 
                 --WFGEL 
                 WGQGTLVT 
               
               
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 RLYLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 24B3 
                 42 
                 VH3-21 
                 D3-10 
                 JH4 
                 EVQLVESGGGLVKPG 
                 TYSMN 
                 WVRQAPGK 
                 FISGRSSY 
                 RFTISRDNAKN 
                 DDWFEEL 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCSK 
                   
                   
               
               
                   
               
               
                 24C9 
                 34 
                 VH3-21 
                 D3-10 
                 JH4 
                 EVQLVESGGGLVKPG 
                 TYSMN 
                 WVRQAPGK 
                 FISGRSSY 
                 RFTISRDNAKN 
                 DDWFEEL 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCSK 
                   
                   
               
               
                   
               
               
                 32G7 
                 38 
                 VH3-21 
                 D3-10 
                 JH4 
                 EVQLVESGGGLVKPG 
                 SYSMN 
                 WVRQAPGK 
                 FISSRSNY 
                 RFTISRDNAKN 
                 DDFEEL 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTIS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCSK 
                   
                   
               
               
                   
               
               
                 33B1 
                 46 
                 VH3-21 
                 D3-10 
                 JH4 
                 EVQLVESGGGLVKPG 
                 SYTMN 
                 WVRQAPGK 
                 FIDSRSSY 
                 RFTISRDNAKN 
                 DDWFEEL 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCSK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 137  
                 Germline 
                 QVQLQESGPGLVKPS 
                 SYYWS 
                 WIRQPAGK 
                 RIYTSGST 
                 RVTMSVDTSKN 
                 ---WNY--- 
                 WGQGTLVT 
               
               
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 NYNPSLKS 
                 QFSLKLSSVTA 
                 FDY 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29A3 
                 102  
                 VH4-4 
                 D1-7 
                 JH4 
                 QVQLQESGPGLVKPS 
                 SYFWS 
                 WIRQPAGK 
                 RIYFSGRT 
                 RVTMSVETSKN 
                 DGGWNYDVAF 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 ETLSLTCTVSGGSIS 
                   
                 GLEWIG 
                 NYNPSLKS 
                 QFSLKLNSVTA 
                 DY 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 ADTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 138  
                 Germline 
                 QVQLVQSGAEVKKPG 
                 SYDIN 
                 WVRQATGQ 
                 WMNPNSGN 
                 RVTMTRNTSIS 
                 -GYS--- 
                 WGQGTLVT 
               
               
                   
                   
                   
                 ASVKVSCKASGYTFT 
                   
                 GLEWMG 
                 TGYAQKFQ 
                 TAYMELSSLRS 
                 WFDP 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29F7 
                 62 
                 VH1-08 
                 D6-13 
                 JH5 
                 QVQLVQSGAEVKKPG 
                 SYDIN 
                 WVRQATGQ 
                 WMNPNSGK 
                 RVTMTRSTSIS 
                 GGYSNLGWFD 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 QSVKVSCKASGYTFT 
                   
                 GLEWMG 
                 TGYAQKFQ 
                 TAYMELSSLRS 
                 P 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
               
                 33D5 
                 58 
                 VH1-08 
                 D6-13 
                 JH5 
                 QVQLVQSGAEVKKPG 
                 SYDIN 
                 WVRQATGQ 
                 WMNPNSGN 
                 RVTLTRNTSIR 
                 GGYSNLGWFD 
                 WGQGSLVT 
               
               
                   
                   
                   
                   
                   
                 ASVKVSCKASGYTFG 
                   
                 GLEWMG 
                 TGYAQKFQ 
                 TVYMELSSLRS 
                 P 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
               
                 29H3 
                 54 
                 VH1-08 
                 D6-13 
                 JH5 
                 QVQLVQSGAEVKKPG 
                 SYDIN 
                 VWRQATGQ 
                 WMNPNSGK 
                 RVTMTRNTSIN 
                 GGYSNLGWFD 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 ASVKVSCKASGYTFT 
                   
                 GLEWMG 
                 TGYAQKFQ 
                 TAYMELSSLRS 
                 P 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 139  
                 Germline 
                 QVQLVESGGGLVKPG 
                 DYYMS 
                 WIRQAPGK 
                 YISSSGST 
                 RFTISRDNAKN 
                 ---YSSGWY- 
                 WGQGTLVT 
               
               
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRA 
                 YFDY 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 21A1 = 
                 66 
                 VH3-11 
                 D6-19 
                 JH4 
                 QVQLVESGGGLVKPG 
                 DYYMS 
                 WIRQAPGK 
                 YISNSGIT 
                 RFTISRDNAKN 
                 EEWYSSSWYR 
                 WGQGTLVT 
               
               
                 21H6 
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRA 
                 NFDY 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 GDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 140  
                 Germline 
                 QVQLVESGGGLVKPG 
                 DYYMS 
                 WIRQAPGK 
                 YISSSGST 
                 RFTISRDNAKN 
                 -DFWSGYY- 
                 WGQGTLVT 
               
               
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRA 
                 NWFDP 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 31E11 
                 70 
                 VH3-11 
                 D3-3 
                 JH5 
                 QVQLVESGGGLVKPG 
                 DYYMS 
                 WIRQAPGK 
                 YISISGST 
                 RFTISRDNAKN 
                 DDFWSGYYFN 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRA 
                 WFDP 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
               
                 33C3 
                 74 
                 VH3-11 
                 D3-3 
                 JH5 
                 QVQLVESGGGLVKPG 
                 DYYMS 
                 WIRQPAGK 
                 YISHGGST 
                 RFTISRDNAKN 
                 DDFWSGYYFN 
                 WGQGALVT 
               
               
                   
                   
                   
                   
                   
                 ESLRLSCAASGFTFS 
                   
                 GLEWVS 
                 IYYADSVK 
                 SLYLQMNSLRV 
                 WFDP 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYFCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 141  
                 Germline 
                 QVQLVESGGGVVQPG 
                 SYGMH 
                 WVRQAPGK 
                 VIWYDGSN 
                 RFTISRDNSKN 
                 --TMVRGVII- 
                 WGQGTTVT 
               
               
                   
                   
                   
                 RSLRLSCAASGFTFS 
                   
                 GLEWVA 
                 KYYADSVK 
                 TLYLQMNSLRA 
                 -YYYGMDV 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 21H9 
                  6 
                 VH3-33 
                 D3-10 
                 JH6 
                 QVPLVESGGGVVQPG 
                 SYGMH 
                 WVRQAPGK 
                 VIWYDGSN 
                 RFTISRDNSKN 
                 GVTMVRGLII 
                 WGQGTTVT 
               
               
                   
                   
                   
                   
                   
                 RSLRLSCAASGFTFS 
                   
                 GLEWVA 
                 KYYADSVQ 
                 TLYLQMNSLRA 
                 ARYYYGLDV 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
               
                 30D7 
                  2 
                 VH3-33 
                 D3-10 
                 JH6 
                 QVPLVESGGGVVQPG 
                 SYGMH 
                 WVRQAPGK 
                 VIWYDGSN 
                 RFTISRDNSKN 
                 GVTMVRGVII 
                 WGQGTTVT 
               
               
                   
                   
                   
                   
                   
                 RSLRLSCAASGFTFS 
                   
                 GLEWVA 
                 KYYADSVK 
                 TLYLQMNSLRA 
                 ARYYYGLDV 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 142  
                 Germline 
                 QVQLVESGGGVVQPG 
                 SYGMH 
                 WVRQAPGK 
                 VIWYDGSN 
                 RFTISRDNSKN 
                 --WND- 
                 WGQGTTVT 
               
               
                   
                   
                   
                 RSLRLSCAASGFTFS 
                   
                 GLEWVA 
                 KYYADSVK 
                 TLYLQMNSLRA 
                 YYYGMDV 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAR 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 30F6 
                 78 
                 VH3-33 
                 D1-20 
                 JH6 
                 QVQLVESGGGVVQPG 
                 TYGMH 
                 WVRQAPGK 
                 VIWYDGSN 
                 RFTISRDNSKN 
                 SYWNDDYYYG 
                 WGQGTTVT 
               
               
                   
                   
                   
                   
                   
                 RSLRLSCAASGFTFS 
                   
                 GLEWVA 
                 KYYADSVK 
                 TLYLQMNSLRA 
                 MDV 
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 143  
                 Germline 
                 EVQLLESGGGLVQPG 
                 SYAMS 
                 WVRQAPGK 
                 AISGSGGS 
                 RFTISRDNSKN 
                 VATDY 
                 WGQGTLVT 
               
               
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 TYYADSVK 
                 TLYLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCAK 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 1G6 
                 131  
                 VH3-23 
                 D5-12 
                 JH4 
                 WVQLLESGGGLVQPG 
                 SYAMS 
                 WVRQAPGK 
                 AISGGGGN 
                 RFTISRDNSKN 
                 VATDY 
                 WGQGTLVT 
               
               
                   
                   
                   
                   
                   
                 GSLRLSCAASGFTFS 
                   
                 GLEWVS 
                 TYYADSVK 
                 TLYLQMNSLRA 
                   
                 VSS 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 G 
                 EDTAVYYCSK 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 21a 
               
             
            
               
                   
               
               
                 Light chain analysis 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 SEQ 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 Chain Name 
                 ID NO: 
                 V 
                 J 
                 FR1 
                 CDR1 
                 FR2 
                 CDR2 
                 FR3 
                 CDR3 
                 FR4 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 121  
                 Germline 
                 DIVMTQTPLSSPV 
                 RSSQSLVHSDGN 
                 WLQQRPGQPPR 
                 KISNRFS 
                 GVPDRFSGSGAGTDFT 
                 GQATQ 
                 FGGGT 
               
               
                   
                   
                   
                 TLGQPASISC 
                 TYLS 
                 LLIY 
                   
                 LKISRVEAEDVGVYYC 
                 FPLT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 30D7 
                  4 
                 A23 
                 JK4 
                 DIVMTQTPLSSPV 
                 RSSQSLVHSDGN 
                 WLQQRPGQPPR 
                 KISNRFF 
                 GVPDRFSGSGAGTDFT 
                 MQSTQ 
                 FGGGT 
               
               
                   
                   
                   
                   
                 TLGQPASISC 
                 TYLS 
                 LLIY 
                   
                 LKIGRVEAEDVGLYYC 
                 FPLT 
                 KVEIK 
               
               
                   
               
               
                 21H9 
                  8 
                 A23 
                 JK4 
                 DIVMTQTPLSSPV 
                 RSSQLSVHSDGN 
                 WLQQRPGQPPR 
                 KISNRFF 
                 GVPDRFSGSGAGTDFT 
                 MQSTQ 
                 FGGGT 
               
               
                   
                   
                   
                   
                 TFGQPASISC 
                 TYLS 
                 LLIY 
                   
                 LKISRVEAEDVGLYYC 
                 FPLT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 122  
                 Germline 
                 EIVLTQSPGTLSL 
                 RASQSVSSSYLA 
                 WYQQKPGQAPR 
                 GASSRAT 
                 GIPDRFSGSGSGTDFT 
                 QQYGS 
                 FGGGT 
               
               
                   
                   
                   
                 SPGERATLSC 
                   
                 LLIY 
                   
                 LTISRLEPEDFAVYYC 
                 SPPLT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 27A3 
                 12 
                 A27 
                 JK4 
                 EIVLTQSPGLTLSL 
                 RASWSVSSSYLA 
                 WYQQKPGQAPR 
                 ATSNRAT 
                 GIPDRFSGSGSGTDFT 
                 QQHGS 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SPGERATLSC 
                   
                 LLIY 
                   
                 LTISRLEPEDFAVYYC 
                 SPPLT 
                 KVEIK 
               
               
                   
               
               
                 27D10 
                 16 
                 A27 
                 JK4 
                 EIVLTQSPGTLSL 
                 RASQSVSSSYLA 
                 WYQQKPGQAPR 
                 GASSRAT 
                 GIPDRFSGSGSGTDFT 
                 QQYGS 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SPGERATLSC 
                   
                 LLIY 
                   
                 LTISRLEPEDFAVYYC 
                 SPPLT 
                 KVEIK 
               
               
                   
               
               
                 32F4 
                 20 
                 A27 
                 JK4 
                 EIVLTQSPGTLSL 
                 RTSQSVSSSYLA 
                 WYQQKPGQAPR 
                 GASSRAT 
                 GVPDRFSGSGSGTDFS 
                 QQYGS 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SPGERATLSC 
                   
                 LLIY 
                   
                 LTISRLEPEDFAVYYC 
                 SPPLT 
                 KVEIK 
               
               
                   
               
               
                 29D4 
                 24 
                 A27 
                 JK4 
                 EIVLTQSPGTLSL 
                 RASQSISRSYLA 
                 WYQQKPGQAPR 
                 GASSRAT 
                 GIPDRFSGSGSGTDFT 
                 QQYGS 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SPGERATLSC 
                   
                 LLIY 
                   
                 LTISRLEPEDFAVYYC 
                 SPPLT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 123  
                 Germline 
                 EIVLTQSPGTLSL 
                 RASQSVSSSYLA 
                 WYQQKPGQAPR 
                 GASSRAT 
                 GIPDRFSGSGSGTDFT 
                 QQYG# 
                 FGQGT 
               
               
                   
                   
                   
                 SPGERATLSC 
                   
                 LLIY 
                   
                 LTISRLEPEDFAVYYC 
                 #IT 
                 RLEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 30A1 
                 28 
                 A27 
                 JK5 
                 EIVLTQSPGTLSL 
                 RASQSISSSYLA 
                 WYQQKPGQAPR 
                 GASSRAT 
                 GIPDRFNGSGSGTDFT 
                 QQLGS 
                 FGQGT 
               
               
                   
                   
                   
                   
                 SPGKRAALSC 
                   
                 LLIY 
                   
                 LTISRLEPEDFAVYYC 
                 SIT 
                 RLEIK 
               
               
                   
               
               
                 22B8 
                 32 
                 A27 
                 JK5 
                 EIVLTQSPGTLSL 
                 RASQSISSSYLA 
                 WYQQRPGQAPR 
                 GASSRAT 
                 GIPDRFNGSGSGTDFT 
                 QQFGS 
                 FGQGT 
               
               
                   
                   
                   
                   
                 SPGERAALSC 
                   
                 LLIY 
                   
                 LTISRLEPEDFAVYYC 
                 SIT 
                 RLEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 124  
                 Germline 
                 DIQMTQSPSSLSA 
                 RASQGRINDLG 
                 WYQQKPGKAPK 
                 AASSLQS 
                 GVPSRFSGSGSGTEFT 
                 LQHNS 
                 FGQGT 
               
               
                   
                   
                   
                 SVGDRVTITC 
                   
                 RLILY 
                   
                 LTISSLQPEDFATYYC 
                 YPWT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 24C9 
                 36 
                 A30 
                 JK1 
                 DIQMTQSPSSLSA 
                 RASQGIRNDLG 
                 WYQQKPGKAPK 
                 ATFSLQS 
                 GVPSRFSGSGSGTEFT 
                 LQHNR 
                 FGQGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 RLIY 
                   
                 LTISSLQPEDFATYYC 
                 YPWT 
                 KVEIK 
               
               
                   
               
               
                 32G7 
                 40 
                 A30 
                 JK1 
                 DIQMTQSPSSLSA 
                 RASQDIRNDLG 
                 WYQQKPGKAPK 
                 AASSLQS 
                 GVPSRFSGSGSGTEFT 
                 LQHNS 
                 FGQGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 RLIY 
                   
                 LTISSLQPEDFATYYC 
                 FPWT 
                 KVEIK 
               
               
                   
               
               
                 24B3 
                 44 
                 A30 
                 JK1 
                 DIQMTQSPSSLSA 
                 RASQGIRSDLG 
                 WYQQKPGKAPK 
                 ATSSLQS 
                 GVPSRFSGSGSGTEFT 
                 LQHNR 
                 FGQGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 RLIY 
                   
                 LTISSLQPEDFATYYC 
                 YPWT 
                 KVEIK 
               
               
                   
               
               
                 33B1 
                 48 
                 A30 
                 JK1 
                 DIQMTQSPSSLSA 
                 RASQGIRNDLG 
                 WYQQKPGKAPK 
                 ATSSLQS 
                 GVPSRFSGSGSGTEFT 
                 LHHNS 
                 DGQGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 RLIY 
                   
                 LTISSLQPEDFATYYC 
                 FPWT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 125  
                 Germline 
                 DIVMTQSPDSLAV 
                 KSSQSVLYSSNN 
                 WYQQKPGQPPK 
                 WASTRES 
                 GVPDRFSGSGSGTDFT 
                 QQYYS 
                 FGQGT 
               
               
                   
                   
                   
                 SLGERATINC 
                 KNYLA 
                 LLIY 
                   
                 LTISSLQAEDVAVYYC 
                 TPWT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 33E1 
                 52 
                 B3 
                 JK1 
                 DIVMTQSPDSLAV 
                 KSSQSILYSSNN 
                 WYQRKPGQPPI 
                 WASTRES 
                 GVPDRFSGSGSRTDFT 
                 QQYFI 
                 FGQGT 
               
               
                   
                   
                   
                   
                 SLGERATINC 
                 KNYLA 
                 LLIH 
                   
                 LTISSLQAEDVAVYYC 
                 TPWT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 126  
                 Germline 
                 DIQMTQSPSSVSA 
                 RASQGISSWLA 
                 WYQQKPGKAPK 
                 AASSLQS 
                 GVPSRFSGSGSGTDFT 
                 QQANS 
                 FGPGT 
               
               
                   
                   
                   
                 SVGDRVTITC 
                   
                 LLIY 
                   
                 LTISSLQPEDFATYYC 
                 FPFT 
                 KVDIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29H3 
                 56 
                 L5 
                 JK3 
                 DIQMTQSPSSVSA 
                 RASQGISSWLV 
                 WYHQKPGKAPK 
                 GASSLQS 
                 GVPSRFSGSGSGTDFT 
                 QQANN 
                 FGPGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 LLIY 
                   
                 LTISSLQPEDFATYYC 
                 FPFT 
                 KVDIK 
               
               
                   
               
               
                 33D5 
                 60 
                 L5 
                 JK3 
                 DIQMTQSPSSVSV 
                 RASQGISSWLA 
                 WYQQKPGKAPK 
                 AASSLQS 
                 GVPSRFSGSGSGTDFT 
                 QQANS 
                 FGPGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 LLIY 
                   
                 LTISSLQPEDFATYYC 
                 FPFT 
                 KVDIK 
               
               
                   
               
               
                 29F7 
                 64 
                 L5 
                 JK3 
                 DIQMTQSPSSVFA 
                 RASQGISTWLA 
                 WYQQKPGKAPK 
                 AASSLQS 
                 GVPSRFSGSGSGTDFT 
                 QQANN 
                 FGPGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 FLIY 
                   
                 LTISSLQPDDFATYYC 
                 FPFT 
                 KVDIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 127  
                 Germline 
                 DIQMTQSPSSLSA 
                 RASQSISSYLN 
                 WYQQKPGKAPK 
                 AASSLQS 
                 GVPSRFSGSGSGTDFT 
                 QQSYS 
                 FGGGT 
               
               
                   
                   
                   
                 SVGDRVTITC 
                   
                 LLIY 
                   
                 LTISSLQPEDFATYYC 
                 T##T 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 21A1 
                 68 
                 O2 
                 JK4 
                 DIQMTQFSSSLSA 
                 RASQSISRYLN 
                 WYQQKPGKAPK 
                 AASSLQS 
                 GVPSRFNSGSGSGTDFT 
                 QQSYS 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 LLIY 
                   
                 LTISSLQPEDFATYYC 
                 APLT 
                 KVEIK 
               
               
                   
               
               
                 21H6 
                 68 
                 O2 
                 JK4 
                 DIQMTQFSSSLSA 
                 RASQSISRYLN 
                 WYQQKPGKAPK 
                 AASSLQS 
                 GVPSRFNGSGSGTDFT 
                 QQSYS 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 LLIY 
                   
                 LTISSLQPEDFATYYC 
                 APLT 
                 KVEIK 
               
               
                   
               
               
                 31E11 
                 72 
                 O2 
                 JK4 
                 DIQMTQFSSSLSA 
                 RANQTISTFLN 
                 WYQQNPGKAPK 
                 AASSLQS 
                 GFPSRFSGSGSTDFT 
                 QQTYI 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 LLIY 
                   
                 LTISSLQPEDFATYYC 
                 IPLT 
                 KVEIK 
               
               
                   
               
               
                 33C3 
                 76 
                 O2 
                 JK4 
                 DIQMTQYPLSLSA 
                 RASQSISSFLN 
                 WYHQKPGKAPK 
                 GTSSLQS 
                 GVPSRFSGSGSGTDFT 
                 QQTYS 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SVGDRVTITC 
                   
                 LLIY 
                   
                 LTISSLQPEDFATYYC 
                 IPLT 
                 NVEIK 
               
               
                   
               
               
                 30F6 
                 80 
                 O2 
                 JK4 
                 DIQMTQSPSSLSA 
                 RASQNISSYLI 
                 WYQQKPGKAPN 
                 TTSSLQR 
                 GVPSRFSGSGSGTDFT 
                 QQSYS 
                 FGGGT 
               
               
                   
                   
                   
                   
                 SVGDRVIITC 
                   
                 LLIH 
                   
                 LTISSLQPEDFATYYC 
                 APLT 
                 KVEIK 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 128  
                 Germline 
                 QSVLTQPPSASGT 
                 SGSSSNIGSNTV 
                 WYQQLPGTAPK 
                 SNNQRPS 
                 GVPDRFSGSKSGTSAS 
                 AAWDDS 
                 FGTGT 
               
               
                   
                   
                   
                 PGQRVTISC 
                 N 
                 LLIY 
                   
                 LAISGLQSEDEADYYC 
                 LNGYV 
                 KVTVL 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 32B2 
                 84 
                 V1-16 
                 JL1 
                 QSVLTQPPSASGT 
                 SGSSSNIGSNTV 
                 WYQQLPGTAPK 
                 LNNQRPS 
                 GVPDRFSGSKSGTSAS 
                 SAWDDS 
                 FGTGT 
               
               
                   
                   
                   
                   
                 PGQRVTISC 
                 N 
                 LLIY 
                   
                 LAISGLQSEDEADYYC 
                 LNGYV 
                 KVTVL 
               
               
                   
               
               
                 30E3 
                 88 
                 V1-16 
                 JL1 
                 QSVLTQPPSASGT 
                 SGSSSNIGSNTV 
                 WYQQLPGTAPK 
                 LNNQRPS 
                 GVPDRFSGSKSGTSAS 
                 SAWDDS 
                 FGTGT 
               
               
                   
                   
                   
                   
                 PGQRVTISC 
                 N 
                 LLIY 
                   
                 LAISGLQSEDEADYYC 
                 LNGYV 
                 KVTVL 
               
               
                   
               
               
                 29A11 
                 92 
                 V1-16 
                 JL1 
                 WSVLTQPPSASGT 
                 SGSSSNIGSNTV 
                 WYQQLPGTAPK 
                 LNNQRPS 
                 GVPDRFSGSKSGTSAS 
                 AAWDDS 
                 FGTGT 
               
               
                   
                   
                   
                   
                 PGQRVTISC 
                 N 
                 LLIY 
                   
                 LAISGLQSEDEADFYC 
                 LNGYV 
                 KVTVL 
               
               
                   
               
               
                 30H10 
                 96 
                 V1-16 
                 JL1 
                 QSVLTQPPSASGT 
                 SGSSSNIGSNTV 
                 WYQQLPGTAPK 
                 LNNQRPS 
                 GVPGRFSGSKSGTSAS 
                 SAWDDS 
                 FGTGT 
               
               
                   
                   
                   
                   
                 PGQRVTISC 
                 N 
                 LLIY 
                   
                 LAISGLQSESEADYYC 
                 LNGYV 
                 KVTVL 
               
               
                   
               
               
                 32C11 
                 100  
                 V1-16 
                 JL1 
                 QSVLTQPPSASGT 
                 SGSSSNIGSNTV 
                 WYHQLPGTAPK 
                 LNNQRPS 
                 GVPDRFSGSKSGTAS 
                 SAWDDS 
                 FGTGT 
               
               
                   
                   
                   
                   
                 PGQRVTISC 
                 N 
                 LLIY 
                   
                 LASIGLQSEDEADYYC 
                 LNGYV 
                 KVTVL 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 129  
                 Germline 
                 QAVLTQPSSLSAS 
                 TLRSGINVGTYR 
                 WYQQKPGSPPQ 
                 YKSDSDK 
                 QQGSGVPSRFSGSKDA 
                 EADYY 
                 FGGGT 
               
               
                   
                   
                   
                 PGASASLTC 
                 IY 
                 YLLR 
                   
                 SANAGILLISGLQSED 
                 GMIWH 
                 KLTVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 S###V 
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29A3 
                 104  
                 V4-2 
                 JL3 
                 QAVLTQPSSLSAS 
                 TLRSGINVGTYR 
                 WYQQKPGSPPQ 
                 YKSDSDQ 
                 LQGSGVPSRFSGSKDA 
                 EADYY 
                 FGGGT 
               
               
                   
                   
                   
                   
                 PGASASLTC 
                 IY 
                 YLLR 
                   
                 SANAGILLISGLQSED 
                 CMVWH 
                 KLTVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 SNTWV 
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 130  
                 Germline 
                 QPVLTQSSSASAS 
                 TLSSGHSSYIIA 
                 WHQQQPGKAPR 
                 LEGSGSY 
                 NKGSGVPDRFSGSSSG 
                 DYYCE 
                 FGGGT 
               
               
                   
                   
                   
                 LGSSVKLTC 
                   
                 YLMK 
                   
                 ADRYLTISNLQFEDEA 
                 TWDSN 
                 KLTVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 TVV 
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 1G6 
                 108  
                 V5-4 
                 JL2 
                 QPVLTQSSSASAS 
                 TLSSGHSSYIIA 
                 WHQQQPGKAPR 
                 LEGSGSY 
                 NKGSGVPDRFSGSSSG 
                 DYYCE 
                 FGGGT 
               
               
                   
                   
                   
                   
                 LGSSVKLTC 
                   
                 YLMK 
                   
                 ADRYLTISNLQFEDEA 
                 TWDSY 
                 KLTVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 TVV 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 21b 
               
             
            
               
                   
               
               
                 Light chain analysis 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 Chain Name 
                 SEQ ID NO: 
                 V 
                 J 
                 FR1 
                 CDR1 
                 FR2 
                 CDR2 
                 FR3 
                 CDR3 
                 FR4 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 144  
                 Germline 
                 DIVMTQSPDSLA 
                 KSSQSVLYS 
                 WYQQKPGQ 
                 WASTRES 
                 GVPDRFSGS 
                 QQYYST 
                 FGQGTKV 
               
               
                   
                   
                   
                 VSLGERATINC 
                 SNNKNYLA 
                 PPKLLIY 
                   
                 GSGTDFTLT 
                 PWT 
                 EIK 
               
               
                   
                   
                   
                   
                   
                   
                   
                 ISSLQAEDV 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 33E1 
                 52 
                 B3 
                 JK1 
                 DIVMTQSPDSLA 
                 KSSQSILYS 
                 WYQRKPGQ 
                 WASTRES 
                 GVPDRFSGS 
                 QQYFIT 
                 FGQGTKV 
               
               
                   
                   
                   
                   
                 VSLGERATINC 
                 SNNKNYLA 
                 PPILLIH 
                   
                 GSRTDFTLT 
                 PWT 
                 EIK 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISSLQAEDV 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 145  
                 Germline 
                 QSVLTQPPSAS 
                 SGSSSNIGS 
                 WYQQLPGT 
                 SNNQRPS 
                 GVPDRFSGS 
                 AAWDDS 
                 FGTGTKV 
               
               
                   
                   
                   
                 GTPGQRVTISC 
                 NTVN 
                 APKLLIY 
                   
                 KSGTSASLA 
                 LNGYV 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                 ISGLQSESE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 ADYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29A11 
                 92 
                 VL 1c 
                 JL1 
                 QSVLTQPPSAS 
                 SGSSSNIGS 
                 WYQQLPGT 
                 LNNQRPS 
                 GVPDRFSGS 
                 AAWDDS 
                 FGTGTKV 
               
               
                   
                   
                   
                   
                 GTPGQRVTISC 
                 NTVN 
                 APKLLIY 
                   
                 KSGTSASLA 
                 LNGYV 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISGLQSESE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ADFYC 
                   
                   
               
               
                   
               
               
                 32B2 
                 84 
                 VL 1c 
                 JL1 
                 QSVLTQPPSAS 
                 SGSSSNIGS 
                 WYQQLPGT 
                 LNNQRPS 
                 GVPDRFSGS 
                 SAWDDS 
                 FGTGTKV 
               
               
                   
                   
                   
                   
                 GTPGQRVTISC 
                 NTVN 
                 APKLLIY 
                   
                 ISGTSASLA 
                 LNGYV 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISGLQSESE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ADYYC 
                   
                   
               
               
                   
               
               
                 30E3 
                 88 
                 VL 1c 
                 JL1 
                 QSVLTQPPSAS 
                 SGSSSNIGS 
                 WYQQLPGT 
                 LNNQRPS 
                 GVPDRFSGS 
                 SAWDDS 
                 FGTGTKV 
               
               
                   
                   
                   
                   
                 GTPGQRVTISC 
                 NTVN 
                 APKLLIY 
                   
                 KSGTSASLA 
                 LNGYN 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISGLQSESE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ADYYC 
                   
                   
               
               
                   
               
               
                 30H10 
                 96 
                 VL 1c 
                 JL1 
                 QSVLTQPPSAS 
                 SGSSSNIGS 
                 WYQQLPGT 
                 LNNQRPS 
                 QVPGRFSGS 
                 SAWDDS 
                 FGTGTKV 
               
               
                   
                   
                   
                   
                 GTPGQRVTISC 
                 NTVN 
                 APKLLIY 
                   
                 KSGTSASLA 
                 LNGYV 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISGLQSESE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ADYYC 
                   
                   
               
               
                   
               
               
                 32C11 
                 100  
                 VL 1c 
                 JL1 
                 QSVLTQPPSAS 
                 SGSSSNIGS 
                 WYHQLPGT 
                 LNNQRPS 
                 GVPDRFSGS 
                 SAWDDS 
                 FGTGTKV 
               
               
                   
                   
                   
                   
                 GTPGQRTVISC 
                 NTVN 
                 APKLLIY 
                 KSGTSASLA 
                 LNGYV 
                 TVL 
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISGLQSESE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ADYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 146  
                 Germline 
                 EIVLTQSPGTL 
                 RASQSVSSS 
                 WYQQKPGQ 
                 GASSRAT 
                 GIPDRFSGS 
                 QQYGSS 
                 FGQGTRL 
               
               
                   
                   
                   
                 SLSPGERATLS 
                 YLA 
                 APRLLIY 
                   
                 GSGTDFTLT 
                 PIT 
                 EIK 
               
               
                   
                   
                   
                 C 
                   
                   
                   
                 ISRLEPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 22B8 
                 32 
                 VK A27 
                 JK5 
                 EIVLTQSPGTL 
                 RASQSISSS 
                 WYQQRPGQ 
                 GASSRAT 
                 GIPDRFNGS 
                 QQFGSS 
                 FGQGTRL 
               
               
                   
                   
                   
                   
                 SLSPGERAALS 
                 YLA 
                 APRLLIY 
                   
                 GSGTDFTLT 
                 -IT 
                 EIK 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISRLEPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
               
                 30A1 
                 28 
                 VK A27 
                 JK5 
                 EIVLTQSPGTL 
                 RASQSISSS 
                 WYQQKPGQ 
                 GASSRAT 
                 GIPDRFNGS 
                 QQLGSS 
                 RGQGTRL 
               
               
                   
                   
                   
                   
                 SLSPGKRAALS 
                 YLA 
                 APRLLIY 
                   
                 GSGTDFTLT 
                 -IT 
                 EIK 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISRLEPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 147  
                 Germline 
                 DIQMTQSPSSL 
                 RASQGIRND 
                 WYQQKPGK 
                 AASSLQS 
                 GVPSRFSGS 
                 LQHNSY 
                 FGQGTKV 
               
               
                   
                   
                   
                 SASVGDRVTIT 
                 LG 
                 APKRLIY 
                   
                 GSGTEFTLT 
                 PWT 
                 EIK 
               
               
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 24B3 
                 44 
                 VK A30 
                 JK1 
                 DIQMTQSPSSL 
                 RASQGIRSD 
                 WYQQKPGK 
                 ATSSLQS 
                 GVPSRFSGS 
                 LQHNRY 
                 FGQGTKV 
               
               
                   
                   
                   
                   
                 SASVGDRVTIT 
                 LG 
                 APKRLIY 
                   
                 GSGTEFTLT 
                 PWT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
               
                 24C9 
                 36 
                 VK A30 
                 JK1 
                 DIQMTQSPSSL 
                 RASQGIRND 
                 WYQQKPGK 
                 ATFSLQS 
                 GVPSRFSGS 
                 LQHNRY 
                 FGQGTKV 
               
               
                   
                   
                   
                   
                 SASVGDRVTIT 
                 LG 
                 APKRLIY 
                   
                 GSGTEFTLT 
                 PWT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
               
                 32G7 
                 40 
                 VK A30 
                 JK1 
                 DQIMTQSPSSL 
                 RASQDIRND 
                 WYQQKPGK 
                 AASSLQS 
                 GVPSRFSGS 
                 QLHNSF 
                 FGQGTKV 
               
               
                   
                   
                   
                   
                 SASVGDRVTIT 
                 LG 
                 APKRLIY 
                   
                 GSGTEFTLT 
                 PWT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
               
                 33B1 
                 48 
                 VK A30 
                 JK1 
                 DIQMTQSPSSL 
                 RASQGIRND 
                 WYQQKPGK 
                 ATSSLQS 
                 GVPSRFSGS 
                 LHHNSF 
                 FGQGTKV 
               
               
                   
                   
                   
                   
                 SASVGDRVTIT 
                 LG 
                 APKRLIY 
                   
                 GSGTEFTLT 
                 PWT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 148  
                 Germline 
                 QAVLTQPASLS 
                 TLRSGINVG 
                 WYQQKPGS 
                 YKSDSDKQQ 
                 GVPSRFSGS 
                 MIWHSS 
                 FGGGTKL 
               
               
                   
                   
                   
                 ASPGASASLTC 
                 TYRIY 
                 PPQYLLR 
                 GS 
                 KDASANAGI 
                 ASV 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                 LLISGLQSE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 DEADYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29A3 
                 104  
                 VL 5c 
                 JL2 + 
                 QAVLTQPSSLS 
                 TLRSGINVG 
                 WYQQKPGS 
                 YKSDSDQLQ 
                 GVPSRFSGS 
                 MVWHSN 
                 FGGGTKL 
               
               
                   
                   
                   
                 JL3 
                 ASPGASASLTC 
                 TYRIY 
                 PPQYLLR 
                 GS 
                 KDASANAGI 
                 TWV 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 LLISGLQSE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 DEADYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 149  
                 Germline 
                 EIVLTQSPGTL 
                 RASQSVSSS 
                 WYQQKPGQ 
                 GASSRAT 
                 GIPDRSSGS 
                 QQYGSS 
                 FGGGTKV 
               
               
                   
                   
                   
                 SLSPGERATLS 
                 YLA 
                 APRLLIY 
                   
                 GSGTFTLT 
                 PPLT 
                 EIK 
               
               
                   
                   
                   
                 C 
                   
                   
                   
                 ISRLEPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 27A3 
                 12 
                 VK A27 
                 JK4 
                 EIVLTQSPGTL 
                 RASQSVSSS 
                 WYQQKPGQ 
                 ATSNRAT 
                 GIPDRFSGS 
                 QQHGSS 
                 FGGGTKV 
               
               
                   
                   
                   
                   
                 SLSPGERATLS 
                 YLA 
                 APRLLIY 
                   
                 GSGTDFTLT 
                 PPLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISRLEPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
               
                 27D10 
                 16 
                 VK A27 
                 JK4 
                 EIVLTQSPGTL 
                 RASQSVSSS 
                 WYQQKPGQ 
                 GASSRAT 
                 GIPDRFSGS 
                 QQYGSS 
                 FGGGTKV 
               
               
                   
                   
                   
                   
                 SLSPGERATLS 
                 YLA 
                 APRLLIY 
                   
                 GSGTDFTLT 
                 PPLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISRLEPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
               
                 32F4 
                 20 
                 VK A27 
                 JK4 
                 EIVLTQSPGTL 
                 RTSQSVSSS 
                 WYQQKPGQ 
                 GASSRAT 
                 GVPDRFSGS 
                 QQYGSS 
                 FGGGTKV 
               
               
                   
                   
                   
                   
                 WLSPGERATLS 
                 YLA 
                 APRLLIY 
                   
                 GSGTDFSLT 
                 PPLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISRLEPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
               
                 29D4 
                 24 
                 VK A27 
                 JK4 
                 EIVLTQSPGTL 
                 RASQSISRS 
                 WYQQKPGQ 
                 GASSRAT 
                 GIPDRFSGS 
                 QQYGSS 
                 FGGGTKV 
               
               
                   
                   
                   
                   
                 SLSPGERATLS 
                 YLA 
                 APRLLIY 
                   
                 GSGTDFTLT 
                 PPLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISRLEPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 AVYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 150  
                 Germline 
                 DIQMTQSPSSV 
                 RASQGISSW 
                 WYQQKPGK 
                 AASSLQS 
                 GVPSRFSGS 
                 QQANSF 
                 FGPGTKV 
               
               
                   
                   
                   
                 SASVGDRVTIT 
                 LA 
                 APKLLIY 
                   
                 GSGTDFTLT 
                 PFT 
                 DIK 
               
               
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 29F7 
                 64 
                 VK L5 
                 JK3 
                 DIQMTQSPSSV 
                 RASQGISTW 
                 WYQQKPGK 
                 AASSLQS 
                 GVPSRFSGS 
                 QQANNF 
                 FGPGTKV 
               
               
                   
                   
                   
                   
                 FASVGDRVTIT 
                 LA 
                 APKFLIY 
                   
                 GSGTDFTLT 
                 PFT 
                 DIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPDDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
               
                 29H3 
                 56 
                 VK L5 
                 JK3 
                 DIQMTQSPSSV 
                 RASQGISSW 
                 WYHQKPGK 
                 GASSLQS 
                 GVPSRFSGS 
                 QQANNF 
                 FGPGTKV 
               
               
                   
                   
                   
                   
                 SASVGDRVTIT 
                 LV 
                 APKLLIY 
                   
                 GSGTDFTLT 
                 PFT 
                 DIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
               
                 33D5 
                 60 
                 VK L5 
                 JK3 
                 DIQMTQSPSSV 
                 RASQGISSW 
                 WYQQKPGK 
                 AASSLQS 
                 GVPSRFSGS 
                 QQANSF 
                 FGPFTKV 
               
               
                   
                   
                   
                   
                 SVSVGDRVTIT 
                 LA 
                 APKLLIY 
                   
                 GSGTDFTLT 
                 PFT 
                 DIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 151  
                 Germline 
                 DIQMTQSPSSL 
                 RASQSISSY 
                 WYQQKPGK 
                 AASSLQS 
                 GVPSRFSGS 
                 QQSYST 
                 FGGGTKV 
               
               
                   
                   
                   
                 SASVGDRVTIT 
                 LN 
                 APKLLIY 
                   
                 GSGTDFTLT 
                 PLT 
                 EIK 
               
               
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 21A1 = 
                 68 
                 VK 
                 JK4 
                 DIQMTQFSSSL 
                 RASQSISRY 
                 WYQQKPGK 
                 AASSLQS 
                 GVPSRFNGS 
                 QQSYSA 
                 FGGGTKV 
               
               
                 21H6 
                   
                 O2 + O12 
                   
                 SASVGDRVTIT 
                 LN 
                 APKLLIY 
                   
                 GSGTDFTLT 
                 PLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
               
                 31E11 
                 72 
                 VK 
                 JK4 
                 DIQMTQSPSSL 
                 RANQTISTF 
                 WYQQNPGK 
                 AASSLQS 
                 GVPSRFSGS 
                 QQTYII 
                 FGGGTKV 
               
               
                   
                   
                 O2 + O12 
                   
                 SASVGDRVTIT 
                 LN 
                 APKLLIY 
                   
                 GSGTDFTLT 
                 PLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
               
                 33C3 
                 76 
                 VK 
                 JK4 
                 DIQMTQYPLSL 
                 RASQSISSF 
                 WYHQKPGK 
                 GTSSLQS 
                 GVPSRFSGS 
                 QQTYSI 
                 FGGGTNV 
               
               
                   
                   
                 O2 + O12 
                   
                 SASVGDRVTIT 
                 LN 
                 APKLLIY 
                   
                 GSGTDFTLT 
                 PLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
               
                 30F6 
                 80 
                 VK 
                 JK4 
                 DIQMTQSPSSL 
                 RASQNISSY 
                 WYQQKPGK 
                 TTSSLQR 
                 GVPSRFSGS 
                 QQSYSA 
                 FGGGTKV 
               
               
                   
                   
                 O2 + O12 
                   
                 SASVGDRVIIT 
                 LI 
                 APNHLIH 
                   
                 GSGTDFTLT 
                 PLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISSLQPEDF 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ATYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 152  
                 Germline 
                 DIVMTQTPLSS 
                 RSSQSLVHS 
                 WLQQRPGQ 
                 KISNRFS 
                 GVPDRFSGS 
                 MQATQF 
                 FGGGTKV 
               
               
                   
                   
                   
                 PVTLGQPASIS 
                 DGNTYLS 
                 PPRLLIY 
                   
                 GAGTDFTLK 
                 PLT 
                 EIK 
               
               
                   
                   
                   
                 C 
                   
                   
                   
                 ISRVEAEDV 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 GVYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 21H9 
                  8 
                 VK A23 
                 JK4 
                 DIVMTQTPLSS 
                 RSSQSLVHS 
                 WLQQRPGQ 
                 KISNRFF 
                 GVPDRFSGS 
                 MQSTQF 
                 FGGGTKV 
               
               
                   
                   
                   
                   
                 PVTFGQPASIS 
                 DGNTYLS 
                 PPRLLIY 
                   
                 GAGTDFTLK 
                 PLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 ISRVEAEDV 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 GLYYC 
                   
                   
               
               
                   
               
               
                 30D7 
                  4 
                 VK A23 
                 JK4 
                 DIVMTQTPLSS 
                 RSSQSLVHS 
                 WLQQGPGQ 
                 KISNRFF 
                 GVPDRFSGS 
                 MQSTQF 
                 FGGGTKV 
               
               
                   
                   
                   
                   
                 PVTLGQPASIS 
                 DGNTYLS 
                 PPRLLIY 
                   
                 GAGTDFTLK 
                 PLT 
                 EIK 
               
               
                   
                   
                   
                   
                 C 
                   
                   
                   
                 IGRVEAEDV 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 GLYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 153  
                 Germline 
                 QPVLTQSSSAS 
                 TLSSGHSSY 
                 WHQQQPGK 
                 LEGSGSYNK 
                 GVPDRFSGS 
                 ETWDSN 
                 FGGGTKL 
               
               
                   
                   
                   
                 ASLGSSVKLTC 
                 IIA 
                 APRYLMK 
                 GS 
                 SSGADRYLT 
                 TVV 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                 ISNLQLEDE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 ADYYC 
                   
                   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 1G6 
                 108  
                 VL 4a 
                 JL2 + 
                 QPVLTQSSSAS 
                 TLSSGHSSY 
                 WHQQQPGK 
                 LEGSGSYNK 
                 GVPDRFSGS 
                 ETWDSY 
                 FGGGTKL 
               
               
                   
                   
                   
                 JL3 
                 ASLGSSVKLTC 
                 IIA 
                 APRYLMK 
                 GS 
                 SSGADRYLT 
                 TVV 
                 TVL 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ISNLQFEDE 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 ADYYC 
               
               
                   
               
            
           
         
       
     
     Example 11 
     Potency Determination of KDR Antibodies 
     The potency of the candidate KDR antibodies was determined to discriminate candidate antibodies based on their ability to prevent the VEGF165-induced tyrosine phosphorylation of KDR in HUVEC cells. This assay was performed with two different conditions, one with a one hour pre-incubation of antibody with HUVEC cells at 4° C. and the second with a 24-hour pre-incubation of antibody with HUVEC cells at 37° C. 
     HUVEC cells were seeded at 25,000 cells/well in supplemented media (EMB-2+2% FCS) and incubated overnight at 37° C. The cells were then incubated overnight in media without supplement at 37° C. On day 3, 50 μL/well of candidate antibody titration or serum-free media (for control) was added to the HUVEC cells. The cells were pre-incubated with candidate antibody for either one hour at 4° C. or for 24 hours at 37° C. After the pre-incubation period, the cells were stimulated with 50 μL of 2 nM VEGF165 (Calbiochem) and subsequently lysed. Cell lysates were then assayed by ELISA. 
     Table 22 shows assay results for the candidate antibodies after a one hour pre-incubation period. Table 23 shows the results for the candidate antibodies after a 24 hour pre-incubation period. 
     
       
         
           
               
             
               
                 TABLE 22 
               
             
            
               
                   
               
               
                 ELISA Results from Inhibition Potency Assay 
               
               
                 (1-hour Pre-incubation at 4° C.) 
               
            
           
           
               
               
            
               
                   
                 Activity at 133 nM 
               
               
                   
                 (% Inhibition) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 MAb ID 
                 N = 1 
                 N = 2 
                 Average 
               
               
                   
                   
               
               
                   
                 33D5 
                 39% 
                 60% 
                 49% 
               
               
                   
                  1G6 
                 35% 
                 50% 
                 43% 
               
               
                   
                 29A11 
                 30% 
                 33% 
                 32% 
               
               
                   
                 32G7 
                 79% 
                 79% 
                 79% 
               
               
                   
                 29D4 
                 64% 
                 73% 
                 68% 
               
               
                   
                 30E3 
                 29% 
                 37% 
                 33% 
               
               
                   
                 33B1 
                 86% 
                 88% 
                 87% 
               
               
                   
                 33C3 
                 78% 
                 83% 
                 80% 
               
               
                   
                 29F7 
                 36% 
                 52% 
                 44% 
               
               
                   
                 27A3 
                 63% 
                 72% 
                 67% 
               
               
                   
                 21H9 
                 21% 
                 31% 
                 26% 
               
               
                   
                 27D10 
                 60% 
                 69% 
                 65% 
               
               
                   
                 32F4 
                 60% 
                 68% 
                 64% 
               
               
                   
                 30D7 
                 26% 
                 28% 
                 27% 
               
               
                   
                 32B2 
                 25% 
                 33% 
                 29% 
               
               
                   
                 22B8 
                 75% 
                 76% 
                 76% 
               
               
                   
                 29H3 
                 45% 
                 55% 
                 50% 
               
               
                   
                 21H6 
                 74% 
                 75% 
                 74% 
               
               
                   
                 30F6 
                 84% 
                 85% 
                 84% 
               
               
                   
                 29A3 
                 25% 
                 20% 
                 23% 
               
               
                   
                 30H10 
                 31% 
                 21% 
                 26% 
               
               
                   
                 24B3 
                 80% 
                 80% 
                 80% 
               
               
                   
                 32C11 
                 32% 
                 21% 
                 27% 
               
               
                   
                 31E11 
                 82% 
                 80% 
                 81% 
               
               
                   
                 33E1 
                 78% 
                 75% 
                 77% 
               
               
                   
                 24C9 
                 81% 
                 no material 
                 81% 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 23 
               
             
            
               
                   
               
               
                 ELISA Results from Inhibition Potency Assay 
               
               
                 (24-hour Pre-incubation at 37° C.) 
               
            
           
           
               
               
            
               
                   
                 Activity at 133 nM 
               
               
                   
                 (% Inhibition) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 MAb ID 
                 N = 1 
                 N = 2 
                 Average 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 33D5 
                 8% 
                 −38% 
                 −15% 
               
               
                   
                  1G6 
                 25% 
                 47% 
                 36% 
               
               
                   
                 29A11 
                 19% 
                 27% 
                 23% 
               
               
                   
                 32G7 
                 36% 
                 77% 
                 57% 
               
               
                   
                 29D4 
                 75% 
                 85% 
                 80% 
               
               
                   
                 30E3 
                 69% 
                 97% 
                 83% 
               
               
                   
                 33B1 
                 88% 
                 82% 
                 85% 
               
               
                   
                 33C3 
                 45% 
                 88% 
                 67% 
               
               
                   
                 29F7 
                 23% 
                 30% 
                 26% 
               
               
                   
                 27A3 
                 19% 
                 0% 
                 9% 
               
               
                   
                 21H9 
                 −3% 
                 2% 
                 −1% 
               
               
                   
                 27D10 
                 19% 
                 −4% 
                 8% 
               
               
                   
                 32F4 
                 25% 
                 18% 
                 22% 
               
               
                   
                 30D7 
                 39% 
                 37% 
                 38% 
               
               
                   
                 32B2 
                 38% 
                 61% 
                 49% 
               
               
                   
                 22B8 
                 82% 
                 115% 
                 99% 
               
               
                   
                 29H3 
                 1% 
                 36% 
                 19% 
               
               
                   
                 21H6 
                 35% 
                 19% 
                 27% 
               
               
                   
                 30F6 
                 60% 
                 51% 
                 55% 
               
               
                   
                 29A3 
                 7% 
                 15% 
                 11% 
               
               
                   
                 30H10 
                 48% 
                 77% 
                 62% 
               
               
                   
                 24B3 
                 76% 
                 69% 
                 72% 
               
               
                   
                 32C11 
                 73% 
                 73% 
                 73% 
               
               
                   
                 31E11 
                 90% 
                 48% 
                 69% 
               
               
                   
                 33E1 
                 85% 
                 68% 
                 77% 
               
               
                   
                 24C9 
                 46% 
                 no material 
                 46% 
               
               
                   
                   
               
            
           
         
       
     
     Example 12 
     Characterization of KDR Antibodies to Deliver Agonist Signal 
     The next assay was conducted in order to characterize the ability of anti-KDR antibodies ability to deliver an agonist signal. 
     HUVEC cells were seeded at 25,000 cells/well in supplemented media (EMB-2+2% FCS) and incubated overnight at 37° C. The media was then replaced with supplement-free media (EMB-2), and the cells were incubated overnight at 37° C. On day 3, the supplement-free media was replaced with 50 μL/well of candidate antibody titration or serum-free media (for control), and the cells were incubated with candidate antibody for one hour at 4° C. After the pre-incubation period, the cells were stimulated with 2 nM VEGF-165 (Calbiochem) and subsequently lysed. Cell lysates were then assayed by ELISA. 
     Table 24 shows assay results for the candidate antibodies as a percentage of phosphorylation activity compared to that induced by VEGF-165. 
     
       
         
           
               
             
               
                 TABLE 24 
               
             
            
               
                   
               
               
                 ELISA Results from Agonist Activity Assay 
               
            
           
           
               
               
            
               
                   
                 % of Max VEGF KDR 
               
               
                   
                 pTyr Activity 
               
               
                   
                 (at 133 nM 
               
               
                   
                 antibody concentration) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 MAb ID 
                 N = 1 
                 N = 2 
                 Average 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 33D5 
                 20% 
                 15% 
                 17% 
               
               
                   
                  1G6 
                 17% 
                 10% 
                 13% 
               
               
                   
                 29A11 
                 15% 
                 10% 
                 12% 
               
               
                   
                 32G7 
                 11% 
                 6% 
                 9% 
               
               
                   
                 29D4 
                 15% 
                 12% 
                 13% 
               
               
                   
                 30E3 
                 11% 
                 8% 
                 10% 
               
               
                   
                 33B1 
                 7% 
                 7% 
                 7% 
               
               
                   
                 33C3 
                 6% 
                 10% 
                 8% 
               
               
                   
                 29F7 
                 19% 
                 12% 
                 16% 
               
               
                   
                 27A3 
                 22% 
                 17% 
                 19% 
               
               
                   
                 21H9 
                 16% 
                 13% 
                 15% 
               
               
                   
                 27D10 
                 19% 
                 17% 
                 18% 
               
               
                   
                 30D7 
                 12% 
                 12% 
                 12% 
               
               
                   
                 32B2 
                 12% 
                 10% 
                 11% 
               
               
                   
                 22B8 
                 9% 
                 5% 
                 7% 
               
               
                   
                 29H3 
                 20% 
                 11% 
                 16% 
               
               
                   
                 21H6 
                 10% 
                 12% 
                 11% 
               
               
                   
                 30F6 
                 14% 
                 13% 
                 13% 
               
               
                   
                 29A3 
                 15% 
                 17% 
                 16% 
               
               
                   
                 30H10 
                 12% 
                 13% 
                 12% 
               
               
                   
                 24B3 
                 12% 
                 15% 
                 14% 
               
               
                   
                 32C11 
                 9% 
                 11% 
                 10% 
               
               
                   
                 31E11 
                 4% 
                 6% 
                 5% 
               
               
                   
                 33E1 
                 5% 
                 10% 
                 8% 
               
               
                   
                 24C9 
                 13% 
                 no material 
                 13% 
               
               
                   
                 2 nM VEGF 
                 100% 
                 100% 
                 100% 
               
               
                   
                 Non- 
                 0% 
                 0% 
                 0% 
               
               
                   
                 Simulated 
               
               
                   
                 IgG2 
                 6% 
                 12% 
                 9% 
               
               
                   
                 IgG4 
                 6% 
                 10% 
                 8% 
               
               
                   
                   
               
            
           
         
       
     
     Example 13 
     Determination of Relative Potency of Purified Antibodies 
     Inhibition of VEGF165-Mediated Survival 
     The relative potencies of the purified candidate antibodies were examined for their ability to block survival of serum-deprived HUVEC cells as mediated by VEGF165. These assays were also performed at a antibody concentrations of 133 nM. All incubations with HUVEC cells took place at 37° C. and 5% CO 2 . 
     HUVEC cells were seeded at 10,000 cells/well and incubated overnight in supplemented media (EBM-2+2% FCS+all supplements except VEGF, see Example 8). The cells were then washed and the candidate antibodies were added to the HUVEC cells and incubated for 2 hours. VEGF165 was added to the cells to a final concentration of 1 nM, and the cells were incubated for 4 days. Cell survival was then measured by addition of luminescent substrate and luminometer assay. Table 25 provides a listing of (n=2) luminometer readings indicating the percentage of HUVEC survival inhibition of VEGF165-mediated KDR activity. 
     
       
         
           
               
             
               
                 TABLE 25 
               
             
            
               
                   
               
               
                 Inhibition of HUVEC Cell Survival by Purified Antibody 
               
               
                 (Measurement by VEGF165-Mediated KDR Activity) 
               
            
           
           
               
               
            
               
                   
                 Activity at 133 nM 
               
               
                   
                 Antibody Concentration 
               
               
                   
                 (% Survival Inhibition) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 MAb ID 
                 N = 1 
                 N = 2 
                 Average 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 33D5 
                 13% 
                 −2% 
                 5% 
               
               
                   
                  1G6 
                 9% 
                 −10% 
                 0% 
               
               
                   
                 29A11 
                 6% 
                 −10% 
                 −2% 
               
               
                   
                 32G7 
                 70% 
                 67% 
                 69% 
               
               
                   
                 29D4 
                 85% 
                 85% 
                 85% 
               
               
                   
                 30E3 
                 −5% 
                 −27% 
                 −16% 
               
               
                   
                 33B1 
                 80% 
                 66% 
                 73% 
               
               
                   
                 33C3 
                 104% 
                 102% 
                 103% 
               
               
                   
                 29F7 
                 3% 
                 −27% 
                 −12% 
               
               
                   
                 27A3 
                 86% 
                 81% 
                 83% 
               
               
                   
                 21H9 
                 −20% 
                 −16% 
                 −18% 
               
               
                   
                 27D10 
                 84% 
                 68% 
                 76% 
               
               
                   
                 32F4 
                 88% 
                 58% 
                 73% 
               
               
                   
                 30D7 
                 −9% 
                 −92% 
                 −51% 
               
               
                   
                 32B2 
                 −13% 
                 −29% 
                 −21% 
               
               
                   
                 22B8 
                 88% 
                 87% 
                 88% 
               
               
                   
                 29H3 
                 −5% 
                 −136% 
                 −70% 
               
               
                   
                 21H6 
                 105% 
                 77% 
                 91% 
               
               
                   
                 30F6 
                 91% 
                 74% 
                 82% 
               
               
                   
                 29A3 
                 −65% 
                 −156% 
                 −111% 
               
               
                   
                 30H10 
                 −84% 
                 −147% 
                 −115% 
               
               
                   
                 24B3 
                 65% 
                 64% 
                 65% 
               
               
                   
                 32C11 
                 −95% 
                 −87% 
                 −91% 
               
               
                   
                 31E11 
                 83% 
                 95% 
                 89% 
               
               
                   
                 33E1 
                 86% 
                 97% 
                 92% 
               
               
                   
                 24C9 
                 57% 
                 no material 
                 57% 
               
               
                   
                   
               
            
           
         
       
     
     Example 14 
     Determination of Relative Potency of Purified Antibodies 
     Inhibition of VEGF-Mediated Release of Prostaglandin 
     The relative potencies of the purified candidate antibodies were also examined for their ability to block release of 6-keto Prostaglandin F 1α  from HUVEC cells as mediated by VEGF165. These assays were also performed at antibody concentrations of 133 nM. All incubations with HUVEC cells took place at 37° C. and 5% CO 2 . 
     HUVEC cells were seeded at 20,000 cells/well and incubated for three days in supplemented media (EBM-2+2% FCS+all supplements except VEGF, see Example 8). The cells were then washed, and the candidate antibodies were added to the HUVEC cells and incubated for 2 hours at 4° C. VEGF165 was added to the cells to a final concentration of 1 nM, and the cells were incubated overnight at 37° C. and 5% CO 2 . Supernatant samples from each well were then measured for release of VEGF165-mediated 6-keto Prostaglandin F 1α . Table 26 shows the results as a percent inhibition of prostaglandin release. 
     
       
         
           
               
             
               
                 TABLE 26 
               
             
            
               
                   
               
               
                 Inhibition of VEGF165-mediated 6-keto Prostaglandin 
               
               
                 F 1α  release in HUVEC Cells by Purified Antibody 
               
            
           
           
               
               
            
               
                   
                 Activity at 133 nM 
               
               
                   
                 Antibody Concentration 
               
               
                   
                 (% Inhibition) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 MAb ID 
                 N = 1 
                 N = 2 
                 Average 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 33D5 
                 15% 
                 57% 
                 36% 
               
               
                   
                  1G6 
                 12% 
                 86% 
                 49% 
               
               
                   
                 29A11 
                 8% 
                 41% 
                 25% 
               
               
                   
                 32G7 
                 37% 
                 115% 
                 76% 
               
               
                   
                 29D4 
                 72% 
                 119% 
                 95% 
               
               
                   
                 30E3 
                 15% 
                 33% 
                 24% 
               
               
                   
                 33B1 
                 76% 
                 114% 
                 95% 
               
               
                   
                 33C3 
                 106% 
                 178% 
                 142% 
               
               
                   
                 29F7 
                 1% 
                 38% 
                 19% 
               
               
                   
                 27A3 
                 54% 
                 121% 
                 88% 
               
               
                   
                 21H9 
                 5% 
                 61% 
                 33% 
               
               
                   
                 27D10 
                 56% 
                 111% 
                 84% 
               
               
                   
                 32F4 
                 69% 
                 76% 
                 73% 
               
               
                   
                 30D7 
                 4% 
                 15% 
                 10% 
               
               
                   
                 32B2 
                 3% 
                 9% 
                 6% 
               
               
                   
                 22B8 
                 67% 
                 76% 
                 72% 
               
               
                   
                 29H3 
                 14% 
                 22% 
                 18% 
               
               
                   
                 21H6 
                 104% 
                 96% 
                 100% 
               
               
                   
                 30F6 
                 68% 
                 89% 
                 79% 
               
               
                   
                 29A3 
                 11% 
                 16% 
                 13% 
               
               
                   
                 30H10 
                 10% 
                 no material 
                 10% 
               
               
                   
                 24B3 
                 66% 
                 70% 
                 68% 
               
               
                   
                 32C11 
                 6% 
                 11% 
                 9% 
               
               
                   
                 31E11 
                 130% 
                 98% 
                 114% 
               
               
                   
                 33E1 
                 128% 
                 98% 
                 113% 
               
               
                   
                 24C9 
                 91% 
                 no material 
                 91% 
               
               
                   
                   
               
            
           
         
       
     
     Based on the data provided the activity comparison experiments as described in this Example and in Examples 11-13 (Tables 22-26), fourteen antibody candidates were carried forward for further analysis. Table 27 provides a summary of the candidate antibodies selected for further study. 
     
       
         
           
               
             
               
                 TABLE 27 
               
             
            
               
                   
               
               
                 Anti-KDR Candidate Antibodies Selected for Further Study 
               
            
           
           
               
               
            
               
                 Selected for 
                   
               
               
                 further study 
                 MAb ID 
               
               
                   
               
               
                 X 
                 21H6 
               
               
                 X 
                 22B8 
               
               
                 X 
                 24B3 
               
               
                 X 
                 27A3 
               
               
                 X 
                 27D10 
               
               
                 X 
                 29D4 
               
               
                 X 
                 29H3 
               
               
                 X 
                 30H10 
               
               
                 X 
                 31E11 
               
               
                 X 
                 32F4 
               
               
                 X 
                 32G7 
               
               
                 X 
                 33E1 
               
               
                 X 
                 33B1 
               
               
                 X 
                 33C3 
               
               
                   
                  1G6 
               
               
                   
                 21H9 
               
               
                   
                 24C9 
               
               
                   
                 29A11 
               
               
                   
                 29A3 
               
               
                   
                 29F7 
               
               
                   
                 30E3 
               
               
                   
                 30D7 
               
               
                   
                 30F6 
               
               
                   
                 32B2 
               
               
                   
                 32C11 
               
               
                   
                 33D5 
               
               
                   
               
            
           
         
       
     
     Example 15 
     Cross-Reactivity of Purified KDR Antibodies to Human VEGF-Receptor-1 (Flt-1) and Mouse KDR (Flk-1) 
     The cross-reactivity of purified antibodies was tested against KDR mouse ortholog KDR mouse ortholog (mouse VEGFR2, or Flk-1) and human VEGFR1 (Flt-1). The ability of the antibodies to bind soluble KDR Ig4-7 domain and to the entire KDR extracellular domain (ECD) was also measured. 
     Cross-reactivity experiments were carried out and measured using an ELISA based assay as described in Example 6. Briefly, titrations of purified antibody (1:10 serial dilutions over 3 data points, starting concentration=1 μg/mL) were added into wells coated with human VEGFR1 (Flt-1, Cat. #321-FL/CF, R&amp;D Systems, Inc.) at 5 μg/mL, mouse KDR (Flk-1, Cat. #443-KD/CF, R&amp;D Systems, Inc.) at 5 μg/mL), soluble KDR Ig4-7 (supernatant, diluted 1:1), or whole KDR-ECD (Cat. # 676490, Calbiochem) at 5 μg/mL. Antibody controls included IgG1 and IgG4 (Sigma-Aldrich, Cat. #15154 and #14639, respectively). Table 28 provides the results of the ELISA cross-reactivity assay for each substrate. 
     
       
         
           
               
             
               
                 TABLE 28 
               
             
            
               
                   
               
               
                 Purified Antibody ELISA Cross-Reactivity Assay Results 
               
            
           
           
               
               
            
               
                   
                 Substrate 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 KDR Ig4-7 
                 KDR-ECD 
                   
               
               
                   
                 Flt-1 (5 μg/mL) 
                 Flk-1 (5 μg/mL) 
                 (1:1 diln.) 
                 (5 ug/mL) 
                 1% PBS/1% milk 
               
               
                   
                 Antibody Conc. 
                 Antibody Conc. 
                 Antibody Conc. 
                 Antibody Conc. 
                 Antibody Conc. 
               
               
                   
                 (μg/mL) 
                 (μg/mL) 
                 (μg/mL) 
                 (μg/mL) 
                 (μg/mL) 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 MAb ID 
                 1 
                 0.1 
                 0.01 
                 1 
                 0.1 
                 0.01 
                 1 
                 0.1 
                 0.01 
                 1 
                 0.1 
                 0.01 
                 1 
                 0.1 
                 0.01 
               
               
                   
               
               
                 27A3 
                 2.483 
                 1.954 
                 1.023 
                 2.571 
                 1.764 
                 0.742 
                 0.078 
                 0.075 
                 0.080 
                 1.973 
                 0.111 
                 0.093 
                 3.109 
                 2.080 
                 0.611 
               
               
                 32F4 
                 2.383 
                 2.013 
                 1.086 
                 2.623 
                 1.998 
                 0.802 
                 0.114 
                 0.079 
                 0.076 
                 1.934 
                 0.132 
                 0.090 
                 3.182 
                 2.334 
                 0.653 
               
               
                 27D10 
                 2.083 
                 1.655 
                 0.858 
                 2.138 
                 1.483 
                 0.537 
                 0.076 
                 0.086 
                 0.073 
                 1.364 
                 0.187 
                 0.087 
                 2.360 
                 1.714 
                 0.441 
               
               
                 21H6 
                 2.449 
                 1.889 
                 0.848 
                 2.722 
                 1.780 
                 0.612 
                 0.087 
                 0.074 
                 0.074 
                 1.458 
                 0.139 
                 0.087 
                 3.303 
                 1.936 
                 0.455 
               
               
                 30H10 
                 2.653 
                 2.467 
                 1.434 
                 2.858 
                 2.643 
                 1.416 
                 3.036 
                 2.772 
                 1.358 
                 2.466 
                 0.310 
                 0.117 
                 3.391 
                 3.161 
                 1.320 
               
               
                 29H3 
                 2.681 
                 2.211 
                 1.224 
                 3.041 
                 2.354 
                 1.138 
                 3.024 
                 2.357 
                 1.140 
                 2.619 
                 0.306 
                 0.099 
                 3.296 
                 2.678 
                 0.967 
               
               
                 32G7 
                 2.406 
                 2.088 
                 1.229 
                 2.672 
                 2.138 
                 0.988 
                 2.654 
                 2.309 
                 0.933 
                 2.362 
                 0.307 
                 0.103 
                 2.979 
                 2.470 
                 0.968 
               
               
                 29D4 
                 2.814 
                 2.420 
                 1.158 
                 3.291 
                 2.590 
                 1.165 
                 0.082 
                 0.078 
                 0.080 
                 2.150 
                 0.096 
                 0.089 
                 3.727 
                 2.855 
                 0.817 
               
               
                 33E1 
                 2.091 
                 1.639 
                 1.043 
                 2.166 
                 1.469 
                 0.846 
                 0.107 
                 0.074 
                 0.067 
                 1.375 
                 0.205 
                 0.081 
                 2.285 
                 1.605 
                 0.714 
               
               
                 31E11 
                 2.846 
                 2.130 
                 0.976 
                 3.100 
                 2.366 
                 0.826 
                 0.107 
                 0.073 
                 0.072 
                 1.963 
                 0.161 
                 0.080 
                 3.604 
                 2.439 
                 0.743 
               
               
                 24B3 
                 2.927 
                 2.601 
                 1.632 
                 3.393 
                 2.755 
                 1.584 
                 3.473 
                 2.802 
                 1.451 
                 3.016 
                 0.304 
                 0.090 
                 4.044 
                 3.003 
                 1.648 
               
               
                 22B8 
                 2.763 
                 2.469 
                 1.367 
                 3.211 
                 2.945 
                 1.202 
                 0.080 
                 0.078 
                 0.074 
                 1.907 
                 0.089 
                 0.075 
                 3.412 
                 2.985 
                 1.131 
               
               
                 33C3 
                 2.790 
                 2.836 
                 1.404 
                 3.475 
                 3.042 
                 1.246 
                 0.094 
                 0.088 
                 0.073 
                 1.115 
                 0.153 
                 0.090 
                 3.825 
                 3.384 
                 1.338 
               
               
                 33B1 
                 2.670 
                 2.081 
                 0.991 
                 3.118 
                 2.105 
                 0.753 
                 3.035 
                 2.137 
                 0.609 
                 1.986 
                 0.178 
                 0.072 
                 3.241 
                 2.303 
                 0.753 
               
               
                 IgG1 
                 0.480 
                 0.471 
                 0.465 
                 0.240 
                 0.224 
                 0.230 
                 0.139 
                 0.097 
                 0.079 
                 0.121 
                 0.087 
                 0.082 
                 0.125 
                 0.084 
                 0.079 
               
               
                 (Control) 
               
               
                 IgG4 
                 0.482 
                 0.495 
                 0.462 
                 0.227 
                 0.223 
                 0.232 
                 0.181 
                 0.078 
                 0.075 
                 0.106 
                 0.078 
                 0.077 
                 0.096 
                 0.075 
                 0.083 
               
               
                 (Control) 
               
               
                   
               
            
           
         
       
     
     Example 16 
     Potency Comparison of Purified KDR Antibodies 
     Inhibition of VEGF-Induced KDR Tyrosine Phosphorylation 
     The relative potency of the purified antibodies was compared by measuring how well the antibodies blocked KDR phosphorylation in an endogenously KDR-expressing normal cell line (HUVEC). The assays were conducted using multiple antibody concentrations and included examination of antibody ability to block VEGF165-mediated KDR tyrosine phosphorylation, ability to block VEGF165-mediated survival of serum-deprived HUVEC, and inhibition of VEGF165-mediated release of 6-keto Prostaglandin F 1α  from HUVEC cells. All incubations with HUVEC cells took place at 37° C. and 5% CO 2  except where indicated. 
     Assay to Measure Inhibition of VEGF165-mediated KDR Activity 
     HUVEC cells were seeded at 25,000 cells/well and incubated overnight in supplemented media (EBM-2+2% FCS+all supplements except VEGF). The cells were then washed and incubated in supplement-free media overnight. On the third day, the various purified antibodies (at 100 nM starting concentration and serially diluted 1:5 to 6 pM final concentration) were added to the HUVEC cells and incubated for one hour at 4° C. The media was then replaced by 50 μL of VEGF165 at 2 nM final concentration. After stimulation with VEGF165 for 10 minutes, the cells were lysed, and the cell lysates were measured for inhibition of VEGF165-mediated KDR activity. Table 29 indicates the level of inhibition of VEGF165-mediated KDR tyrosine phosphorylation as EC50 values and as percentage inhibition values. 
                     TABLE 29                  Inhibition of VEGF165-mediated KDR tyrosine phosphorylation                                 Maximum Inhibition               at 100 nM           EC50 (nM)   (% Inhibition)                                         MAb ID   N = 1   N = 2   Average   N = 1   N = 2   Average               27A3   3.4   2.9   3.1   77%   79%   78%       32F4   1.7   1.9   1.8   71%   79%   75%       27D10   1.6   2.3   1.9   70%   75%   72%       21H6   7.1   6.7   6.9   81%   84%   82%       30H10   0.6   0.2   0.4   36%   22%   29%       29H3   0.5   0.4   0.4   33%   30%   32%       32G7   0.6   0.6   0.6   69%   77%   73%       29D4   2.0   1.9   1.9   74%   77%   75%       33E1   5.8   5.1   5.5   73%   75%   74%       31E11   2.0   1.9   1.9   84%   86%   85%       24B3   2.1   2.3   2.2   78%   84%   81%       22B8   2.7   3.4   3.1   75%   79%   77%       33C3   1.2   1.1   1.2   83%   86%   85%       33B1   1.2   1.1   1.1   86%   86%   86%                    
Assay to Measure Ability of Antibody to Block VEGF 165-mediated Survival of Serum-deprived HUVEC Cells
 
     HUVEC cells were seeded at 10,000 cells/well and incubated overnight in supplemented media (EBM-2+2% FCS+all supplements except VEGF). The cells were then washed and the various purified antibodies were added to the HUVEC cells (at 200 nM starting concentration and serially diluted 1:4 to 13 pM final concentration) and incubated for 2 hours at 4° C. VEGF165 was added to the cells to a final concentration of 1 nM, and the cells were incubated for three days. Cell survival was then measured by addition of luminescent substrate and luminometer assay. Table 30 indicates HUVEC survival by VEGF165-mediated KDR activity as EC50 values and as percent inhibition of survival. 
                     TABLE 30                  Survival of HUVEC Cells by VEGF165-mediated KDR Activity                                 Maximum Inhibition               at 50 nM (%           EC50   Inhibition)                                         MAb ID   N = 1   N = 2   Average   N = 1   N = 2   Average                                                 27A3   3.3   1.9   2.6   95%   94%   95%       32F4   2.3   1.0   1.7   91%   94%   92%       27D10   2.3   2.0   2.2   93%   85%   89%       21H6   2.5   2.3   2.4   96%   96%   96%       30H10   ***   ***   ***   −9%   −1%   −5%       29H3   ***   ***   ***   4%   18%   11%       32G7   0.4   1.4   0.9   73%   72%   72%       29D4   1.1   2.9   2.0   89%   95%   92%       33E1   1.7   1.2   1.4   107%   99%   103%       31E11   2.0   2.8   2.4   101%   101%   101%       24B3   2.9   4.1   3.5   97%   82%   90%       22B8   2.1   2.4   2.2   80%   95%   87%       33C3   0.7   1.4   1.1   106%   95%   101%       33B1   1.7   3.1   2.4   55%   59%   57%               ***EC50 data not presented as KDR was not sufficiently inhibited.            
Assay to Measure Ability of Antibody to Inhibit VEGF165-mediated release of 6-keto Prostaglandin F 1α  from HUVEC cells
 
     HUVEC cells were seeded at 20,000 cells/well and incubated for three days in supplemented media (EBM-2+2% FCS+all supplements except VEGF). The cells were then washed, and the candidate antibodies were added to the HUVEC cells (at 333 nM starting concentration and serially diluted 1:5 to 4 pM final concentration) and incubated for 2 hours at 4° C. VEGF165 was added to the cells to a final concentration of 1 nM, and the cells were incubated overnight. Supernatant samples from each well were then measured for release of VEGF165-mediated 6-keto Prostaglandin F 1α . Table 31 shows the results as a EC50 values and as percent inhibition of prostaglandin release. 
     
       
         
           
               
             
               
                 TABLE 31 
               
             
            
               
                   
               
               
                 Inhibition of VEGF165-mediated 6-keto Prostaglandin F 1α  release 
               
               
                 in HUVEC Cells by Purified Antibody 
               
            
           
           
               
               
               
            
               
                   
                   
                 Maximum Inhibition 
               
               
                   
                   
                 (% Inhibition) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 EC50 (nM) 
                 N = 1 
                 N = 2 
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 MAb ID 
                 N = 1 
                 N = 2 
                 Average 
                 (200 nM) 
                 (333 nM) 
                 Average 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 27A3 
                 5.5 
                 5.7 
                 5.6 
                 49% 
                 49% 
                 49% 
               
               
                 32F4 
                 4.4 
                 4.6 
                 4.5 
                 42% 
                 54% 
                 48% 
               
               
                 27D10 
                 4.5 
                 5.2 
                 4.8 
                 46% 
                 51% 
                 49% 
               
               
                 21H6 
                 7.3 
                 5.4 
                 6.3 
                 80% 
                 75% 
                 77% 
               
               
                 30H10 
                 *** 
                 *** 
                 *** 
                 0% 
                 6% 
                 3% 
               
               
                 29H3 
                 *** 
                 *** 
                 *** 
                 2% 
                 8% 
                 5% 
               
               
                 32G7 
                 11.2 
                 35.1 
                 23.1 
                 28% 
                 55% 
                 42% 
               
               
                 29D4 
                 3.2 
                 3.2 
                 3.2 
                 45% 
                 67% 
                 56% 
               
               
                 33E1 
                 4.8 
                 5.4 
                 5.1 
                 77% 
                 103% 
                 90% 
               
               
                 31E11 
                 6.1 
                 6.4 
                 6.2 
                 88% 
                 108% 
                 98% 
               
               
                 24B3 
                 8.1 
                 16.2 
                 12.1 
                 37% 
                 72% 
                 54% 
               
               
                 22B8 
                 5.1 
                 5.6 
                 5.3 
                 63% 
                 76% 
                 70% 
               
               
                 33C3 
                 3.5 
                 4.4 
                 3.9 
                 76% 
                 94% 
                 85% 
               
               
                 33B1 
                 17.0 
                 7673.0 
                 3845.0 
                 31% 
                 76% 
                 53% 
               
               
                   
               
               
                 ***EC50 data not presented as KDR was not sufficiently inhibited. 
               
            
           
         
       
     
     Example 17 
     Determination of Binding Affinity of Purified Antibodies 
     The binding affinities of the purified antibodies for endogenously-expressed KDR in HUVEC cells was measured. HUVEC cells were seeded at 150,000 cells/well and incubated with titrations of purified antibody for 4 hours at 4° C. The cells were then washed and incubated with goat anti-human IgG-Fc-Cy5+5 μg/mL 7-Amino-Actinomycin (7AAD) for 30 minutes at 4° C. Bound KDR was detected using FACS analysis. Table 32 lists the FACS data obtained from analysis of the HUVEC cells with purified antibodies. 
     
       
         
           
               
             
               
                 TABLE 32 
               
             
            
               
                   
               
               
                 Binding Affinity/Avidity of Purified Antigens to 
               
               
                 KDR in HUVEC cells 
               
            
           
           
               
               
               
            
               
                   
                 MAb ID 
                 FACS Kd (nM) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 27A3 
                 5.4 
               
               
                   
                 32F4 
                 1.8 
               
               
                   
                 27D10 
                 1.9 
               
               
                   
                 21H6 
                 17.9 
               
               
                   
                 30H10 
                 0.9 
               
               
                   
                 29H3 
                 0.5 
               
               
                   
                 32G7 
                 0.2 
               
               
                   
                 29D4 
                 1.0 
               
               
                   
                 33E1 
                 3.8 
               
               
                   
                 31E11 
                 11.3 
               
               
                   
                 24B3 
                 0.8 
               
               
                   
                 22B8 
                 1.8 
               
               
                   
                 33C3 
                 1.0 
               
               
                   
                 33B1 
                 0.2 
               
               
                   
                   
               
            
           
         
       
     
     Based on the data provided potency experiments and data as described in this Example and in Examples 16 (Tables 29-32), eleven antibody candidates were carried forward for further analysis. Table 33 provides a summary of the candidate antibodies selected for further study. 
     
       
         
           
               
             
               
                 TABLE 33 
               
             
            
               
                   
               
               
                 Anti-KDR Candidate Antibodies Selected for Further Study 
               
               
                 (Based on Potency Data) 
               
            
           
           
               
               
            
               
                 Selected for 
                   
               
               
                 further study 
                 MAb ID 
               
               
                   
               
               
                 X 
                 27A3 
               
               
                 X 
                 32F4 
               
               
                 X 
                 27D10 
               
               
                 X 
                 21H6 
               
               
                 X 
                 29D4 
               
               
                 X 
                 33E1 
               
               
                 X 
                 31E11 
               
               
                 X 
                 24B3 
               
               
                 X 
                 22B8 
               
               
                 X 
                 33C3 
               
               
                 X 
                 33B1 
               
               
                   
                 30H10 
               
               
                   
                 29H3 
               
               
                   
                 32G7 
               
               
                   
               
            
           
         
       
     
     Example 18 
     Cross-Reactivity of Purified KDR Antibodies to Non-Human Primates 
     KDR derived from cynomolgus monkey was cloned and expressed on the surface of HEK 293T cells. The binding of purified antibodies to cell-bound cynomolgus KDR (with parental cells as negative control) was tested by FACS analysis in this assay. 
     Cynomolgus KDR was cloned in 3 pieces. One piece was PCR amplified from cynomolgus lung cDNA, and the remaining two pieces were PCR amplified from cynomolgus kidney cDNA. The three PCR products were inserted into pCR3.1 Bid vector. HEK 293T cells were then transfected with cynomologus KDR expression vector or with empty pCR3.1 Bid vector. Transfected cells were seeded at 50,000 cells/well and incubated with 5 μg/mL of candidate antibody for one hour at 4° C. The cells were then washed and incubated with secondary antibody (Cy5-conjugated goat anti-human antibody, or Cy5-conjugated rabbit anti-goat antibody, plus 7-Amino-Actinomycin (7AAD)) for 15 minutes at 4° C. Binding between cynomolgus KDR and purified antibody was detected by FACS analysis. Table 34 provides a summary of the results for the assay. 
     
       
         
           
               
             
               
                 TABLE 34 
               
             
            
               
                   
               
               
                 Cyno Cross-Reactivity Assay Results for Purified Antibodies 
               
            
           
           
               
               
               
            
               
                   
                 FACS Geometric 
                   
               
               
                   
                 Mean Fluorescence 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Cyno KDR/293T 
                 Mock 293T 
               
               
                   
                 MAb ID 
                 Transfectants 
                 Transfectants 
               
               
                   
                   
               
            
           
           
               
               
               
               
            
               
                   
                 27A3 
                 89.7 
                 2.8 
               
               
                   
                 32F4 
                 104.6 
                 2.8 
               
               
                   
                 27D10 
                 89.4 
                 2.8 
               
               
                   
                 21H6 
                 101.7 
                 2.8 
               
               
                   
                 29D4 
                 115.3 
                 2.6 
               
               
                   
                 33E1 
                 88.5 
                 2.7 
               
               
                   
                 31E11 
                 99.5 
                 2.8 
               
               
                   
                 24B3 
                 68.4 
                 2.7 
               
               
                   
                 22B8 
                 103.2 
                 2.7 
               
               
                   
                 33C3 
                 108.1 
                 2.7 
               
               
                   
                 33B1 
                 107.6 
                 2.8 
               
               
                   
                   
               
            
           
         
       
     
     Example 19 
     Determination of Relative Potency of Purified Antibodies 
     Ability to Block VEGF Binding to KDR 
     The relative potency of the various antibodies was assayed by how well the antibodies blocked human VEGF binding to KDR. HEK 293T cells were transfected with human KDR (courtesy of Amgen Fremont, Fremont Calif.) or with empty pCR3.1 Bid vector. Transfected cells were then seeded at 50,000 cells/well and incubated with 5 μg/mL of candidate antibody for one hour at 4° C. The antibodies were subsequently removed, and the cells were and incubated VEGF165 (Cat. # 293-VE, R&amp;D Systems, Inc.) at a concentration of 50 ng/mL for one hour at 4° C. The cells were then washed and incubated with goat anti-VEGF for one hour at 4° C. After washing, the cells were incubated with Cy5-conjugated rabbit anti-goat antibody plus 7AAD for 15 minutes at 4° C. Inhibition of VEGF binding to KDR was detected by FACS analysis. Table 35 provides a summary of the results for the assay. 
     
       
         
           
               
             
               
                 TABLE 35 
               
             
            
               
                   
               
               
                 FACS Results for Inhibition of VEGF Binding to Human KDR 
               
            
           
           
               
               
               
            
               
                   
                 FACS Geometric Mean 
                   
               
               
                 MAb ID 
                 Fluorescence 
                 % Inhibition 
               
               
                   
               
            
           
           
               
               
               
            
               
                 27A3 
                 118 
                 9% 
               
               
                 32F4 
                 112 
                 14% 
               
               
                 27D10 
                 111 
                 15% 
               
               
                 21H6 
                 133 
                 −3% 
               
               
                 29D4 
                 116 
                 11% 
               
               
                 33E1 
                 147 
                 −15% 
               
               
                 31E11 
                 138 
                 −8% 
               
               
                 24B3 
                 97 
                 27% 
               
               
                 22B8 
                 120 
                 7% 
               
               
                 33C3 
                 126 
                 2% 
               
               
                 33B1 
                 81 
                 40% 
               
               
                 293T/KDR transfectants 
                 129 
               
               
                 (control) 
               
               
                 293T/mock transfectants 
                 10 
               
               
                 (control) 
               
               
                   
               
            
           
         
       
     
     The above results show these antibodies have a differentiated mode of action compared to IMC1121b (Lu et al, JBC 2003, 278, 43496). 
     Example 20 
     Determination of Relative Potency of Purified Antibodies 
     Ability to Block VEGF-C Mediated KDR Activity 
     The anti-KDR candidate antibodies were assayed for their ability to inhibit VEGF-C-mediated KDR tyrosine phosphorylation in HUVEC cells. HUVEC cells were seeded at 25,000 cells/well and incubated overnight in supplemented media (EBM-2+2% FCS+all supplements except VEGF). The cells were then washed and incubated in supplement-free media overnight. On the third day, the various purified antibodies were added to the HUVEC cells (at 1:10 serial dilution over three data points, starting concentration=100 nM) and incubated for 2 hours at 4° C. The supernatant volumes were then removed and replaced by 50 μof VEGF-C at 50 nM final concentration for 10 minutes at 37° C. After stimulation VEGF-C, the cells were lysed, and the cell lysates were measured for inhibition of VEGF-C-mediated KDR activity by ELISA assay. Table 36 provides the results of the assay indicating the level of inhibition of VEGF-C-mediated KDR tyrosine phosphorylation. 
     
       
         
           
               
             
               
                 TABLE 36 
               
             
            
               
                   
               
               
                 Inhibition of VEGF-C-mediated KDR Activity 
               
            
           
           
               
               
               
               
            
               
                   
                 % Inhibition (N = 1) 
                 % Inhibition (N = 2) 
                 % Inhibition (Average) 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                 MAb ID 
                 100 nM 
                 10 nM 
                 1 nM 
                 100 nM 
                 10 nM 
                 1 nM 
                 100 nM 
                 10 nM 
                 1 nM 
               
               
                   
               
               
                 27A3 
                 73% 
                 75% 
                 40% 
                 77% 
                 80% 
                 38% 
                 75% 
                 78% 
                 39% 
               
               
                 32F4 
                 75% 
                 76% 
                 55% 
                 86% 
                 79% 
                 31% 
                 81% 
                 77% 
                 43% 
               
               
                 27D10 
                 76% 
                 73% 
                 52% 
                 84% 
                 79% 
                 50% 
                 80% 
                 76% 
                 51% 
               
               
                 21H6 
                 93% 
                 78% 
                 23% 
                 95% 
                 90% 
                 34% 
                 94% 
                 84% 
                 28% 
               
               
                 29D4 
                 74% 
                 71% 
                 42% 
                 79% 
                 72% 
                 25% 
                 76% 
                 71% 
                 34% 
               
               
                 33 E1 
                 83% 
                 75% 
                 29% 
                 75% 
                 64% 
                 36% 
                 79% 
                 69% 
                 32% 
               
               
                 31 E11 
                 84% 
                 81% 
                 47% 
                 84% 
                 85% 
                 33% 
                 84% 
                 83% 
                 40% 
               
               
                 24B3 
                 67% 
                 61% 
                 26% 
                 75% 
                 62% 
                 34% 
                 71% 
                 62% 
                 30% 
               
               
                 22B8 
                 71% 
                 74% 
                 38% 
                 77% 
                 56% 
                 17% 
                 74% 
                 65% 
                 27% 
               
               
                 33C3 
                 84% 
                 87% 
                 71% 
                 86% 
                 89% 
                 61% 
                 85% 
                 88% 
                 66% 
               
               
                 33B1 
                 64% 
                 61% 
                 45% 
                 73% 
                 62% 
                 53% 
                 68% 
                 62% 
                 49% 
               
               
                   
               
            
           
         
       
     
     Example 21 
     Determination of Binding Affinities of Purified Antibodies by Biacore 
     Each purified anti-KDR antibody was immobilized on a CM4 sensor chip within a Biacore 2000 using standard amine coupling. Immobilization levels were kept between 250 and 350 RU. The concentration of KDR was determined by UV-VIS spectroscopy using a molar absorptivity at 280 nm of 110, 440 M −1 cm −1 , which was calculated from the sequence of the protein using a method developed by Pace et al. (G. R. Grimsley and C. N. Pace (2003) in Current Protocols in Protein Science (John Wiley &amp; Sons, Inc.), 3.1.1-3.1.9). The antigen KDR was diluted to a starting concentration of 52 nM and tested in a 3-fold dilution series in triplicate. The running buffer contained HBS-P with 0.1 mg/ml BSA and binding responses were collected at 23 degrees C. Bound complexes were regenerated with a 12 second pulse of 146 mM of phosphoric acid. The response data were globally fit with a simple 1:1 interaction model. The binding constants are provided in the table below. The number shown in parentheses is the standard error in the last significant figure. 
     
       
         
           
               
               
               
               
             
               
                   
                   
               
               
                   
                 ka (M−1s−1) 
                 kd (s−1) 
                 Kd 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 27D10 
                 5.176(6)e4 
                  2.54(1)e−4 
                  4.91(2) nM 
               
               
                   
                 24B3 
                 1.750(1)e5 
                 1.907(8)e−4 
                 1.089(4) nM 
               
               
                   
                 33C3 
                 1.0214(8)e5  
                  9.3(1)e−5 
                  910(10) pM 
               
               
                   
                   
               
               
                   
                 ka (M−1s−1) = k on . 
               
               
                   
                 kd (s−1) = k off   
               
            
           
         
       
     
     Example 22 
     Determination of Cross Competition for KDR by Purified Anti-KDR Antibodies 
     Each purified anti-KDR antibody was tested for its ability to block binding of other anti-KDR antibodies to human KDR using a cell adhesion assay. 
     Wells of 96 well plates were coated over night at 4° C. with 33C3, 24B3 or 27D10 at a concentration of 10 μg/ml PBS. The wells were then blocked with PBS/3% BSA for an hour at 37° C. and washed with PBS. Titrations of purified anti-KDR antibody were tested against each coating antibody for cross competition to KDR, using a maximum concentration of 25 μg/ml. Isotype controls IgG1 and IgG2 (Sigma-Aldrich, Cat #I5154 and I5404 respectively) were included. Antibody titrations were prepared in serum free Hams F12 media at 10 times the final assay concentration and 10 μl was added to triplicate test wells. Porcine Aortic Endothelial cells transfected with human KDR were added at a density of 100,000 cells per well in 90 μl of serum free Hams F12 media. The plates were incubated at 37° C., 5% CO 2  for 1 hour. Non-adhered cells were flicked from the plates and the wells washed twice with PBS. The adhered cells were fixed with 100% ethanol for 30 minutes at room temperature and then stained with 0.1% crystal violet in 1.5% methanol, for 15 minutes at room temperature. Excess stain was washed off with water and the stain within the cells solubilised with 100 ul per well of 0.1% triton X-100 in double distilled water for 2 hours on an orbital platform. The OD at 570 nm was measured and the percent inhibition of binding by each antibody calculated. 
     The table indicates the cross competition between each of the antibodies for human KDR as percent inhibition of cell binding to coating antibody by 25 μg/ml of inhibiting antibody. 
     
       
         
           
               
               
               
            
               
                   
                   
               
               
                   
                 Inhibiting Antibody 
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 33C3 
                 24B3 
                 27D10 
                 IgG2 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Coating 
                 33C3 
                 100 
                 29 
                 98 
                 0 
               
               
                   
                 Antibody 
                 24B3 
                 −9 
                 102 
                 −14 
                 0 
               
               
                   
                   
                 27D10 
                 103 
                 20 
                 103 
                 0 
               
               
                   
                   
               
            
           
         
       
     
     Example 23 
     KDR Inhibitory Antibodies Reduce Tube Formation In Vitro 
     KDR inhibitory antibodies were tested for the ability to reduce endothelial cell tube formation in an in vitro co-culture assay (TCS Cell Works Cat no. ZHA-1000). On day 1, Human Umbilical Vein Endothelial Cells (HUVECs) and human diploid fibroblasts were obtained as co-cultures in 24 well plates. KDR blocking antibodies were introduced to the cultures on day 1 and at regular intervals over an 11-day period at the following concentrations: 20 μg/mL, 5 μg/mL, 1.25 μg/mL and 0.3125 μg/mL. Media was replenished on days 4, 7 and 9. The co-culture model was maintained in either TCS Optimised medium (supplied with the co-culture assay) or in MCDB131 medium supplemented with 2% foetal calf serum (FCS), 1% glutamine and 1% penicillin/streptomycin (hereafter referred to as 2% FS MCDB131 medium). The co-culture model was maintained at 37° C. in a humidified 5% CO 2 /95% air atmosphere. 
     Tubule formation was examined at day 11 following fixing and staining of tubules for CD31 using a tubule staining kit according to the manufacturors instructions (TCS Cell Works Cat no. ZHA-1225). Briefly, cells were fixed with ice-cold 70% ethanol for 30 minutes at room temperature (RT). Cells were blocked after which they were treated with anti-human CD31 for 60 minutes at RT. Plates were washed and treated with goat anti-mouse IgG conjugated with alkaline phosphatase (AP) for 60 minutes at RT. After incubation with the AP-conjugated secondary antibody, the plates were washed and 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT) substrate was added for approximately 10 minutes. The development of a dark purple colour within 10 minutes reflected tubule formation. Plates were subsequently washed and left to air dry. 
     Quantification of tubule growth was conducted by whole-well image analysis methodology using a Zeiss KS400 3.0 Image Analyser. The morphological parameter measured in the quantification methodology was total tubule length. All tubule formations within each of the 24 wells were measured excluding a rim of 100 μm depth to avoid edge retraction artifact. 
     As illustrated in  FIG. 1 , it was observed that the antibodies are effective in inhibiting endothelial cell tube formation in vitro. The data indicate that the antibodies are active in a functional assay that models the angiogenic process. 
     Example 24 
     Determination of In Vivo Efficacy of Purified Antibodies 
     Evaluation of the Antiangiogenic Efficacy in a Spheroid-Based In Vivo Angiogenesis Assay 
     Human umbilical vein endothelial cell (HUVEC) spheroids were prepared as described earlier (Korff and Augustin: J Cell Biol 143: 1341-52, 1998) by pipetting 100 endothelial cells (EC) in a hanging drop on plastic dishes to allow overnight spheroid formation. The following day, using the method previously described (Alajati et al: Nature Methods 5:439-445, 2008), EC spheroids were harvested and mixed in a Matrigel/fibrin solution with single HUVECs to reach a final number of 100,000 ECs as spheroids and 200,000 single ECs per injected plug. VEGF-A and FGF-2 were added at a final concentration of 1000 ng/ml. Male SCID mice (5-8 weeks old) were subcutaneously injected with 500 μl of the cell/matrix suspension. The following day (day 1) treatment commenced. At day 21 the study was terminated. The matrix plugs were removed and fixed in 4% PFA. All matrix plugs were paraffin embedded and cut to a thickness of 8-10 μm for histological examination. Blood vessels were visualized by staining for human CD34 and smooth muscle actin (SMA) and the vessel density and pericyte coverage was determined 
     As illustrated in  FIG. 2 , the antibodies are effective in inhibiting vessel formation in vivo. The data indicate that the antibodies are active in an in vivo assay of angiogenesis. 
     Example 25 
     Inhibition of Tumour Cell Growth in Human Patients 
     A group of human cancer patients diagnosed with pancreatic cancer is randomized into treatment groups. Each patient group is treated with weekly intravenous injections of fully human monoclonal antibodies against KDR as described herein. Each patient is dosed with an effective amount of the antibody ranging from 5 mg/kg/week to 15 mg/kg/week for 4-8 months. A control group is given only the standard chemotherapeutic regimen. 
     At periodic times during and after the treatment regimen, tumour burden is assessed by magnetic resonance imaging (MRI). It can be expected that the patients who have received weekly antibody treatments will show significant reductions in tumour size, time delay to progression or prolonged survival compared to patients that do not receive antibody treatment. In some treated patients, it can be expected that the tumours are no longer detectable. In contrast, it can be expected that tumour size increases or remains substantially the same in the control group. 
     Example 26 
     Inhibition of Colon Cancer in a Human Patient 
     A group of human cancer patients diagnosed with colon cancer is randomized into treatment groups. Each patient group is treated 3-weekly with intravenous injections of fully human monoclonal antibodies against KDR as described herein. Each patient is dosed with an effective amount of the antibody ranging from 5 mg/kg/week to 15 mg/kg/week for 4-8 months. A control group is given only the standard chemotherapeutic regimen. At periodic times during and after the treatment regimen, tumour burden is assessed by magnetic resonance imaging (MRI). It can be expected that the patients who have received 3-weekly antibody treatments show significant reductions in tumour size, time delay to progression or prolonged survival compared to patients that do not receive the antibody treatment. In some treated patients, it can be expected that the tumours are no longer detectable. In contrast, it can be expected that tumour size increases or remains substantially the same in the control group. 
     Example 27 
     Inhibition of Melanoma in a Human Patient 
     A group of human cancer patients diagnosed with melanoma is randomized into treatment groups. Each patient group is treated 3-weekly with intravenous injections of fully human monoclonal antibodies against KDR as described herein. Each patient is dosed with an effective amount of the antibody ranging from 5 mg/kg/week to 15 mg/kg/week for 4-8 months. A control group is given only the standard chemotherapeutic regimen. At periodic times during and after the treatment regimen, tumour burden is assessed by magnetic resonance imaging (MRI). It can be expected that the patients who have received 3-weekly antibody treatments with antibodies against KDR show significant reductions in melanoma, time delay to progression or prolonged survival compared to patients that do not receive the antibody treatment. In some treated patients, it can be expected that the melanoma lesions are no longer detectable. In contrast, it can be expected that melanoma increases or remains substantially the same in the control group. 
     Example 28 
     Inhibition of Chronic Myelogenous Leukemia (CML) in a Human Patient 
     A group of human cancer patients diagnosed with CML is randomized into treatment groups. Each patient group is treated 3-weekly with intravenous injections of fully human monoclonal antibodies against KDR as described herein. Each patient is dosed with an effective amount of the antibody ranging from 5 mg/kg/week to 15 mg/kg/week for 4-8 months. A control group is given only the standard chemotherapeutic regimen. At periodic times during and after the treatment regimen, tumour burden is assessed by magnetic resonance imaging (MRI). It can be expected that the patients who have received 3-weekly antibody treatments show significant reductions in CML, time delay to progression or prolonged survival compared to patients that do not receive the antibody treatment. In some treated patients, it can be expected that the CML is no longer detectable. In contrast, it can be expected that CML increases or remains substantially the same in the control group. 
     Example 29 
     Inhibition of Tumour Cell Growth in a Human Patient 
     A human patient is diagnosed with a malignant tumour. The patient is treated with weekly intravenous injections of fully human monoclonal antibodies against KDR as described herein for 8 weeks. At periodic times during and after the treatment regimen, tumour burden is assessed by magnetic resonance imaging (MRI). It can be expected that significant reductions in tumour size are found. 
     INCORPORATION BY REFERENCE 
     All references cited herein, including patents, patent applications, papers, text books, and the like, and the references cited therein, to the extent that they are not already, are hereby incorporated herein by reference in their entirety. 
     EQUIVALENTS 
     The foregoing written specification is considered to be sufficient to enable one skilled in the art to practice the invention. The foregoing description and Examples detail certain preferred embodiments of the invention and describes the best mode contemplated by the inventors. It will be appreciated, however, that no matter how detailed the foregoing may appear in text, the invention may be practiced in many ways and the invention should be construed in accordance with the appended claims and any equivalents thereof.