Patent Publication Number: US-2007123531-A1

Title: 4-Bromo-5-(2-chloro-benzoylamino)-1h-pyrazole-3-carboxylic acid (phenyl) amide derivatives and related compounds as bradykinin b1 receptor antagonists for the treatment of inflammatory diseases

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS  
      This application claims the benefit of U.S. Provisional Application Ser. No. 60/467,695, filed on May 2, 2003 and U.S. Provisional Application Ser. No. 60/503,652, filed on Sep. 17, 2003, which applications are incorporated herein in their entirety. 
    
    
     BACKGROUND OF THE INVENTION  
      1. Field of the Invention  
      This invention is directed to certain 3-amido-5-substituted pyrazole derivatives and related compounds. These compounds are useful as bradykinin B 1  receptor antagonists to relieve adverse symptoms in mammals mediated, at least in part, by bradykinin B 1  receptor including pain, inflammation, septic shock, the scarring process, etc.  
     REFERENCES  
      The following literature and patent publications are cited in this application as superscript numbers. 
       1  J. G. Menke, et al.,  J. Biol. Chem.,  269(34):21583-21586 (1994).      2  J. F. Hess,  Biochem. Human B   2    Receptor, Biophys. Res. Commun.,  184:260-268 (1992).      3  R. M. Burch, et al., “Bradykinin Receptor Antagonists”,  J. Med. Chem.,  30:237-269 (1990).      4  Clark, W. G. “Kinins and the Peripheral Central Nervous Systems”, Handbook of Experimental Pharmacology, Vol. XXV: Bradykinin, Kallidin, and Kallikrein. Erdo, E. G. (Ed.), 311-322 (1979).      5  Ammons, W. S., et al., “Effects of Intracardiac Bradykinin on T 2 -T 5  Medial Spinothalamic Cells”,  American Journal of Physiology,  249, R145-152 (1985).      6  Costello, A. H. et al., “Suppression of Carageenan-Induced Hyperalgesia, Hyperthermia and Edema by a Bradykinin Antagonist”,  European Journal of Pharmacology,  171:259-263 (1989).      7  Laneuville, et al., “Bradykinin Analogue Blocks Bradykinin-induced Inhibition of a Spinal Nociceptive Reflex in the Rat”,  European Journal of Pharmacology,  137:281-285 (1987).      8  Steranka, et al., “Antinociceptive Effects of Bradykinin Antagonists”,  European Journal of Pharmacology,  136:261-262 (1987).      9  Steranka, et al., “Bradykinin as a Pain Mediator: Receptors are Localized to Sensory Neurons, and Antagonists have Analgesic Actions”,  Neurobiology,  85:3245-3249 (1987).      10  Whalley, et al., in  Naunyn Schmiederberg&#39;s Arch. Pharmacol.,  336:652-655 (1987).      11  Back, et al., “Determination of Components of the Kallikrein-Kinin System in the Cerebrospinal Fluid of Patients with Various Diseases”,  Res. Clin. Stud. Headaches,  3:219-226 (1972).      12  Ness, et al., “Visceral pain: a Review of Experimental Studies”,  Pain,  41: 167-234 (1990).      13  Aasen, et al., “Plasma kallikrein Activity and Prekallikrein Levels during Endotoxin Shock in Dogs”,  Eur. Surg.,  10:5062(1977).      14  Aasen, et al., “Plasma Kallikrein-Kinin System in Septicemia”,  Arch. Surg.,  118:343-346 (1983).      15  Katori, et al., “Evidence for the Involvement of a Plasma Kallikrein/Kinin System in the Immediate Hypotension Produced by Endotoxin in Anaesthetized Rats”,  Br. J. Pharmacol.,  98:1383-1391 (1989).      16  Marceau, et al., “Pharmacology of Kinins: Their Relevance to Tissue Injury and Inflammation”,  Gen. Pharmacol.,  14:209-229 (1982).      17  Weipert, et al.,  Brit J. Pharm.,  94:282-284 (1988).      18  Haberland, “The Role of Kininogenases, Kinin Formation and Kininogenase Inhibitor in Post Traumatic Shock and Related Conditions”,  Klinische Woochen - Schrift,  56:325-331 (1978).      19  Ellis, et al., “Inhibition of Bradykinin- and Kallikrein-Induced Cerebral Arteriolar Dilation by Specific Bradykinin Antagonist”,  Stroke,  18:792-795 (1987).      20  Kamitani, et al., “Evidence for a Possible Role of the Brain Kallikrein-Kinin System in the Modulation of the Cerebral Circulation”,  Circ. Res.,  57:545-552 (1985).      21  Barnes, “Inflammatory Mediator Receptors and Asthma”,  Am. Rev. Respir. Dis.,  135:S26-S31 (1987).      23  Fuller, et al., “Bradykinin-induced Bronchoconstriction in Humans”,  Am. Rev. Respir. Dis.,  135:176-180 (1987).      24  Jin, et al., “Inhibition of Bradykinin-Induced Bronchoconstriction in the Guinea-Pig by a Synthetic B 2  Receptor Antagonist”,  Br. J. Pharmacol.,  97:598-602 (1989).      25  Polosa, et al., “Contribution of Histamine and Prostanoids to Bronchoconstriction Provoked by Inhaled Bradykinin in Atopic Asthma”, Allergy, 45:174-182 (1990).      26  Baumgarten, et al., “Concentrations of Glandular Kallikrein in Human Nasal Secretions Increase During Experimentally Induced Allergic Rhinitis”,  J. Immunology,  137:1323-1328 (1986).      27  Proud, et al., “Nasal Provocation with Bradykinin Induces Symptoms of Rhinitis and a Sore Throat”,  Am. Rev. Respir Dis.,  137:613-616 (1988).      28  Steward and Vavrek in “Chemistry of Peptide Bradykinin Antagonists”  Basic and Chemical Research , R. M. Burch (Ed.), pages 51-96 (1991).      29  Seabrook, et al., Expression of B1 and B2 Bradykinin Receptor mRNA and Their Functional Roles in Sympathetic Ganglia and Sensory Dorsal Root Ganglia Neurons from Wild-type and B2 Receptor Knockout Mice,  Neuropharmacology,  36(7):1009-17 (1997)      30  Elguero, et al., Nonconventional Analgesics: Bradykinin Antagonists,  An. R. Acad. Farm., L 3(1):173-90 (Spa) (1997)      31  McManus, U.S. Pat. No. 3,654,275, Quinoxalinecarboxamide Antiinfiammatory Agents, issued Apr. 4, 1972      32  Beyreuther, B.; et al., International Patent application publication number WO 03/007958 A1 filed on Jul. 4, 2002.      33  Marceau, “Kinin B 1  Receptors: A Review,”  Immunopharmacology,  30:1-26 (1995).      34  Giese, et al., U.S. Pat. No. 5,916,908, issued Jun. 29, 1999      35  Yoshida, et al, Japanese Patent Application Serial No. 49100080      36  Oxford Dictionary of Biochemistry and Molecular Biology. Oxford University Press, 2001.    

      All of the above-identified publications are herein incorporated by reference in their entirety to the same extent as if each individual publication was specifically and individually incorporated by reference in its entirety.  
      2. State of the Art  
      Bradykinin or kinin-9 (BK) is a vasoactive nonapeptide, H-Arg 1 -Pro 2 -Pro 3 -Gly 4 -Phel 5 -Ser 6 -Pro 7 -Phe 8 -Arg 9 -OH (SEQ. ID. NO. 1), formed by the action of plasma kallikrein, which hydrolyses the sequence out of the plasma globulin kininogen. Plasma kallikrein circulates as an inactive zymogen, from which active kallikrein is released by Hageman factor. Tissue kallikrein appears to be located predominantly on the outer surface of epithelial cell membranes at sites thought to be involved in transcellular electrolyte transport.  
      Glandular kallikrein cleaves kininogen one residue earlier to give the decapeptide Lys-bradykinin (kallidin, Lys-BK) (SEQ. ID. NO. 2). Met-Lys-bradykinin (SEQ. ID. NO. 3) is also formed, perhaps by the action of leukocyte kallikrein. Pharmacologically important analogues include des-Arg 9  or BK 1-8  (Amino acids 1-8 of SEQ. ID. NO. 1) and Ile-Ser-bradykinin (or T-kinin) (SEQ. ID. NO. 4), [Hyp 3 ]bradykinin (SEQ. ID. NO. 5), and [Hyp 4 ]bradykinin (SEQ. ID. NO. 6). 36    
      Bradykinin (BK) (SEQ. ID. NO. 1) is known to be one of the most potent naturally occurring stimulators of C-fiber afferents mediating pain. It also is a powerful blood-vessel dilator, increasing vascular permeability and causing a fall in blood pressure, edema-producing agent, and stimulator of various vascular and non-vascular smooth muscles in tissues such as uterus, gut and bronchiole. Bradykinin (SEQ. ID. NO. 1) is formed in a variety of inflammatory conditions and in experimental anaphylactic shock. The kinin/kininogen activation pathway has also been described as playing a pivotal role in a variety of physiologic and pathophysiologic processes, being one of the first systems to be activated in the inflammatory response and one of the most potent simulators of: (i) phospholipase A 2  and, hence, the generation of prostaglandins and leukotrienes; and (ii) phospholipase C and thus, the release of inositol phosphates and diacylgylcerol. These effects are mediated predominantly via activation of BK receptors of the BK 2  type.  
      Bradykinin receptor is any membrane protein that binds bradykinin (BK) (SEQ. ID. NO. 1) and mediates its intracellular effects. Two types of receptors are recognized: B 1 , on which order of potency is des-Arg 9 -bradykinin (BK 1-8 ) (Amino acids 1-8 of SEQ. ID. NO. 1)=kallidin (Lys-BK) (SEQ. ID. NO. 2)&gt;BK (SEQ. ID. NO. 1); and B 2 , with order of potency kallidin (SEQ. ID. NO. 2)&gt;BK (SEQ. ID. NO. 1)&gt;&gt;BK 1-8  (Amino acids 1-8 of SEQ. ID. NO. 1). Hence, BK 1-8  (Amino acids 1-8 of SEQ. ID. NO. 1) is a powerful discriminator. 36  B 1  receptors are considerably less common than B 2  receptors, which are present in most tissues. The rat B 2  receptor is a seven-transmembrane-domain protein which has been shown on activation to stimulate phosphoinositide turnover. The B 1  subtype is induced by inflammatory processes. 33  The distribution of receptor B 1  is very limited since this receptor is only expressed during states of inflammation. Bradykinin receptors have been cloned for different species, notably the human B 1  receptor (see J. G. Menke et al. 1 , and human B2 receptor J. F. Hess 2 ). Examples: B 1 , database code BRB1_HUMAN, 353 amino acids (40.00 kDa); B 2 , database code BRB2_HUMAN, 364 amino acids (41.44 kDa). 36    
      Two major kinin precursor proteins, high molecular weight and low molecular weight kininogen are synthesized in the liver, circulate in plasma, and are found in secretions such as urine and nasal fluid. High molecular weight kininogen is cleaved by plasma kallikrein, yielding BK (SEQ. ID. NO. 1), or by tissue kallikrein, yielding kallidin. Low molecular weight kininogen, however, is a substrate only for tissue kallikrein. In addition, some conversion of kallidin to BK (SEQ. ID. NO. 1) may occur inasmuch as the amino terminal lysine residue of kallidin (SEQ. ID. NO. 2) is removed by plasma aminopeptidases. Plasma half-lives for kinins are approximately 15 seconds, with a single passage through the pulmonary vascular bed resulting in 80-90% destruction. The principle catabolic enzyme in vascular beds is the dipeptidyl carboxypeptidase kininase II or angiotensin-converting enzyme (ACE). A slower acting enzyme, kininase I, or carboxypeptidase N, which removes the carboxyl terminal Arg, circulates in plasma in great abundance. This suggests that it may be the more important catabolic enzyme physiologically. Des-Arg 9 -bradykinin (Amino acids 1-8 of SEQ. ID. NO. 1) as well as des-Arg 10 -kallidin (Amino acids 1-9 of SEQ. ID. NO. 2) formed by kininase I acting on BK (SEQ. ID. NO. 1) or kallidin (SEQ. ID. NO. 2), respectively, are acting BK 1  receptor agonists, but are relatively inactive at the more abundant BK 2  receptor at which both BK (SEQ. ID. NO. 1) and kallidin (SEQ. ID. NO. 2) are potent agonists.  
      Direct application of bradykinin (SEQ. ID. NO. 1) to denuded skin or intra-arterial or visceral injection results in the sensation of pain in mammals including humans. Kinin-like materials have been isolated from inflammatory sites produced by a variety of stimuli. In addition, bradykinin receptors have been localized to nociceptive peripheral nerve pathways and BK (SEQ. ID. NO. 1) has been demonstrated to stimulate central fibers mediating pain sensation. Bradykinin (SEQ. ID. NO. 1) has also been shown to be capable of causing hyperalgesia in animal models of pain. See, Burch, et al, 3  and Clark, W. G. 4    
      These observations have led to considerable attention being focused on the use of BK antagonists as analgesics. A number of studies have demonstrated that bradykinin antagonists are capable of blocking or ameliorating both pain as well as hyperalgesia in mammals including humans. See, Ammons, W. S., et al. 5 , Clark, W. G. 4 , Costello, A. H., et al. 6 , Laneuville, et al. 7 , Steranka, et al. 8  and Steranka, et al. 9    
      Currently accepted therapeutic approaches to analgesia have significant limitations. While mild to moderate pain can be alleviated with the use of non-steroidal anti-inflammatory drugs and other mild analgesics, severe pain such as that accompanying surgical procedures, burns and severe trauma requires the use of narcotic analgesics. These drugs carry the limitations of abuse potential, physical and psychological dependence, altered mental status and respiratory depression which significantly limit their usefulness.  
      Prior efforts in the field of BK antagonists indicate that such antagonists can be useful in a variety of roles. These include use in the treatment of burns, perioperative pain, migraine and other forms of pain, shock, central nervous system injury, asthma, rhinitis, premature labor, inflammatory arthritis, inflammatory bowel disease, neuropathic pain, etc. For example, Whalley, et al. 10  has demonstrated that BK antagonists are capable of blocking BK-induced pain in a human blister base model. This suggests that topical application of such antagonists would be capable of inhibiting pain in burned skin, e.g., in severely burned patients that require large doses of narcotics over long periods of time and for the local treatment of relatively minor burns or other forms of local skin injury.  
      The management of perioperative pain requires the use of adequate doses of narcotic analgesics to alleviate pain while not inducing excessive respiratory depression. Post-operative narcotic-induced hypoventilation predisposes patients to collapse of segments of the lungs, a common cause of post-operative fever, and frequently delays discontinuation of mechanical ventilation. The availability of a potent non-narcotic parenteral analgesic could be a significant addition to the treatment of perioperative pain. While no currently available BK antagonist has the appropriate pharmacodynamic profile to be used for the management of chronic pain, frequent dosing and continuous infusions are already commonly used by anesthesiologists and surgeons in the management of perioperative pain.  
      Several lines of evidence suggest that the kallikrein/kinin pathway may be involved in the initiation or amplification of vascular reactivity and sterile inflammation in migraine. (See, Back, et al. 11 ). Because of the limited success of both prophylactic and non-narcotic therapeutic regimens for migraine as well as the potential for narcotic dependence in these patients, the use of BK antagonists offers a highly desirable alternative approach to the therapy of migraine.  
      Bradykinin (SEQ. ID. NO. 1) is produced during tissue injury and can be found in coronary sinus blood after experimental occlusion of the coronary arteries. In addition, when directly injected into the peritoneal cavity, BK (SEQ. ID. NO. 1) produces a visceral type of pain. (See, Ness, et al. 12 ). While multiple other mediators are also clearly involved in the production of pain and hyperalgesia in settings other than those described above, it is also believed that antagonists of BK (SEQ. ID. NO. 1) have a place in the alleviation of such forms of pain as well.  
      Shock related to bacterial infections is a major health problem. It is estimated that 400,000 cases of bacterial sepsis occur in the United States yearly; of those, 200,000 progress to shock, and 50% of these patients die. Current therapy is supportive, with some suggestion in recent studies that monoclonal antibodies to Gram-negative endotoxin may have a positive effect on disease outcome. Mortality is still high, even in the face of this specific therapy, and a significant percentage of patients with sepsis are infected with Gram-positive organisms that would not be amenable to anti-endotoxin therapy.  
      Multiple studies have suggested a role for the kallikrein/kinin system in the production of shock associated with endotoxin. See, Aasen, et al. 13 , Aasen, et al. 14 , Katori, et al. 15  and Marceau, et al. 16  Recent studies using newly available BK antagonists have demonstrated in animal models that these compounds can profoundly affect the progress of endotoxic shock. (See, Weipert, et al. 17 ). Less data is available regarding the role of BK (SEQ. ID. NO. 1) and other mediators in the production of septic shock due to Gram-positive organisms. However, it appears likely that similar mechanisms are involved. Shock secondary to trauma, while frequently due to blood loss, is also accompanied by activation of the kallikrein/kinin system. (See, Haberland. 18 )  
      Numerous studies have also demonstrated significant levels of activity of the kallikrein/kinin system in the brain. Both kallikrein and BK (SEQ. ID. NO. 1) dilate cerebral vessels in animal models of central nervous system (CNS) injury. (See Ellis, et al. 19  and Kamitani, et al. 20 ). Bradykinin antagonists have also been shown to reduce cerebral edema in animals after brain trauma. Based on the above, it is believed that BK antagonists should be useful in the management of stroke and head trauma.  
      Other studies have demonstrated that BK receptors are present in the lung, that BK (SEQ. ID. NO. 1) can cause bronchoconstriction in both animals and man and that a heightened sensitivity to the bronchoconstrictive effect of BK (SEQ. ID. NO. 1) is present in asthmatics. Some studies have been able to demonstrate inhibition of both BK (SEQ. ID. NO. 1) and allergen-induced bronchoconstriction in animal models using BK antagonists. These studies indicate a potential role for the use of BK antagonists as clinical agents in the treatment of asthma. (See Barnes 21 , Burch, et al. 3 , Fuller, et al. 23 , Jin, et al. 24  and Polosa, et al. 25 .) Bradykinin has also been implicated in the production of histamine and prostanoids to bronchoconstriction provoked by inhaled bradykinin in atopic asthma.  25  BK (SEQ. ID. NO. 1) has also been implicated in the production of symptoms in both allergic and viral rhinitis. These studies include the demonstration of both kallikrein and BK (SEQ. ID. NO. 1) in nasal lavage fluids and that levels of these substances correlate well with symptoms of rhinitis. (See, Baumgarten, et al. 26 , Jin, et al. 24 , and Proud, et al. 27 )  
      In addition, studies have demonstrated that BK (SEQ. ID. NO. 1) itself can cause symptoms of rhinitis. Stewart and Vavrek 28  discuss peptide BK antagonists and their possible use against effects of BK (SEQ. ID. NO. 1). A great deal of research effort has been expended towards developing such antagonists with improved properties. However, notwithstanding extensive efforts to find such improved BK antagonists, there remains a need for additional and more effective BK antagonists. Two of the major problems with presently available BK antagonists are their low levels of potency and their extremely short durations of activity. Thus there is a special need for BK antagonists having increased potency and for duration of action.  
      Two generations of peptidic antagonists of the B2 receptor have been developed. The second generation has compounds two orders of magnitude more potent as analgesics than first generation compounds and the most important derivative was icatibant. The first non-peptidic antagonist of the B2 receptor, described in 1993, has two phosphonium cations separated by a modified amino acid. Many derivatives of this di-cationic compound have been prepared. Another non-peptidic compound antagonist of B2 is the natural product Martinelline. See Elguero, et al., 30  and Seabrook. 29    
      U.S. Pat. No. 3,654,275 31  teaches that certain 1,2,3,4-tetrahydro-1-acyl-3-oxo-2-quinoxalinecarboxamides have anti-inflammatory activity.  
      International Patent Application WO 03/007958 filed on Jul. 2, 2002 and published on Jan. 30, 2003 discloses tetrahydroquinoxalines acting as bradykinin antagonists.  32    
      U.S. Pat. No. 5,916,908 34  teaches the use of 3,5-disubstituted pyrazoles or 3,4,5-trisubstituted pyrazoles as kinase inhibitors.  
      Japanese Patent Application Serial No. 49100080 35  teaches 2-aminopyrazoles as anti-inflammatory agents.  
      Currently there is no marketed therapeutic agent for the inhibition of bradykinin B 1  receptor. In view of the above, compounds which are bradykinin B 1  receptor antagonists would be particularly advantageous in treating those diseases mediated by bradykinin B 1  receptor.  
     SUMMARY OF THE INVENTION  
      This invention is directed, in part, to compounds that are bradykinin B 1  receptor antagonist. It is also directed to compounds that are useful for treating diseases or relieving adverse symptoms associated with disease conditions in mammals, where the disease is mediated at least in part by bradykinin B 1  receptor. For example, inhibition of the bradykinin B 1  receptor is believed to be useful for the moderation of pain, inflammation, septic shock, the scarring process, etc. These compounds are preferably selective for antagonism of the B 1  receptor over the B 2  receptor. Certain of the compounds exhibit increased potency and are expected to also exhibit an increased duration of action.  
      In one embodiment, this invention provides compounds of Formula (I) or Formula (II):  
                 
 
 wherein 
          Z′ is selected from O, S and NH;     R 1  is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic;     R 2  is selected from the group consisting of hydrogen, alkyl, and substituted alkyl;     R 3  is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic;     R 4  is selected from the group consisting of aryl, substituted aryl, heteroaryl, and substituted heteroaryl;     R 5  is selected from the group consisting of hydrogen, alkyl and substituted alkyl;     X is selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkoxy, substituted alkoxy, aryl, substituted aryl, carboxyl, carboxyl esters, cyano, halo, heteroaryl, substituted heteroaryl, hydroxy, nitro, amino, substituted amino, acylamino, and aminoacyl;     or pharmaceutically acceptable salts, prodrugs or isomers thereof; with the following provisos:     A) when Z′ is O, X is H, R 1  is methyl, R 2  is H, R 3  is methyl, and R 5  is H, then R 4  is not N 2 -methyl-3-carboxy-pyrazol-5-yl or N 2 -methyl-3-(methoxycarbonyl)-pyrazol-5-yl;     B) when Z′ is O, X is H, R 2  is H, R 3  is methyl, R 5  is H, and R 1  is either N 2 -methyl-3-[2-(N,N-dimethylamino)eth-1-ylamino]pyrazol-5-yl or N 1 -methyl-3-[2-(N,N-dimethylamino)eth-1-ylamino]pyrazol-5-yl, then R 4  is not N 2 -methyl-3-[2-(N,N-dimethylamino)eth-1-ylamino]pyrazol-5-yl or N 1 -methyl-3-[2-(N,N-dimethylamino)eth-1-ylamino]pyrazol-5-yl;     C) when Z′ is O, X is 2-benzothiazolyl, R 1  is methyl, R 2  is H, R 3  is 4-methylphenyl, and R 5  is H, then R 4  is not phenyl; 
 
 and further with the proviso that the compound is Formula (I) is not 
    A′) 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid phenylamide.        

      In Formula (I) or Formula (II), Z′ is preferably O.  
      In Formula (I) or Formula (I) preferred R 1  groups include aryl and substituted aryl groups. Some examples of aryl groups include phenyl, naphth-2-yl, naphth-1-yl; and the like. Some preferred substituted aryl groups include monosubstituted phenyls, disubstituted phenyls and trisubstituted phenyls such as 5-dimethylaminonaphth-1-yl, 2-chlorophenyl, 2-fluorophenyl, 2-bromophenyl, 2-hydroxyphenyl, 2-nitrophenyl, 2-methylphenyl, 2-methoxyphenyl, 2-phenoxyphenyl, 2-trifluoromethylphenyl, 4-fluorophenyl, 4-chlorophenyl, 4-bromophenyl, 4-nitrophenyl, 4-methylphenyl, 4-hydroxyphenyl, 4-methoxyphenyl, 4-ethoxyphenyl, 4-butoxyphenyl, 4-iso-propylphenyl, 4-phenoxyphenyl, 4-trifluoromethylphenyl, 4-hydroxymethylphenyl, 3-methoxyphenyl, 3-hydroxyphenyl, 3-nitrophenyl, 3-fluorophenyl, 3-chlorophenyl, 3-bromophenyl, 3-phenoxyphenyl, 3-thiomethoxyphenyl, 3-methylphenyl, 3-trifluoromethylphenyl, 2,3-dichlorophenyl, 2,3-difluorophenyl, 2,4-dichlorophenyl, 2,5-dimethoxyphenyl, 3,4-dichlorophenyl, 3,4-difluorophenyl, 3,4-methylenedioxyphenyl, 3,4-dimethoxyphenyl, 3,5-difluorophenyl, 3,5-dichlorophenyl, 3,5-di-(trifluoromethyl)phenyl, 3,5-dimethoxyphenyl, 2,4-dichlorophenyl, 2,4-difluorophenyl, 2,6-difluorophenyl, 3,4,5-trifluorophenyl, 3,4,5-trimethoxyphenyl, 3,4,5-tri-(trifluoromethyl)phenyl, 2,4,6-trifluorophenyl, 2,4,6-trimethylphenyl, 2,4,6-tri-(trifluoromethyl)phenyl, 2,3,5-trifluorophenyl, 2,4,5-trifluorophenyl, 2,5-difluorophenyl, 2-fluoro-3-trifluoromethylphenyl, 4-fluoro-2-trifluoromethylphenyl, 2-fluoro-4-trifluoromethylphenyl, 4-benzyloxyphenyl, 2-chloro-6-fluorophenyl, 2,3,4,5,6-pentafluorophenyl, 2,5-dimethylphenyl, 4-phenylphenyl and 2-fluoro-3-trifluoromethylphenyl, 2-(quinolin-8-yl)thiomethyl)phenyl, 2-((3-methylphen-1-ylthio)methyl)phenyl, and the like.  
      Preferred R 1  substituted aryl groups are alkaryl groups which include, by way of example, benzyl, 2-phenylethyl, 3-phenyl-n-propyl, and the like.  
      Preferred R 1  alkyl, substituted alkyl, alkenyl, cycloalkyl and cycloalkenyl groups in Formula (I) or Formula (II) include, by way of example, iso-propyl, n-propyl, n-butyl, iso-butyl, sec-butyl, t-butyl, —CH 2 CH═CH 2 , —CH 2 CH═CH(CH 2 ) 4 CH 3 , cyclopropyl, cyclobutyl, cyclohexyl, cyclopentyl, cyclohex-1-enyl, —CH 2 -cyclopropyl, —CH 2 -cyclobutyl, —CH 2 -cyclohexyl, —CH 2 -cyclopentyl, —CH 2 CH 2 -cyclopropyl, —CH 2 CH 2 -cyclobutyl, —CH 2 CH 2 -cyclohexyl, —CH 2 CH 2 -cyclopentyl, and the like.  
      Preferred R 1  heteroaryls and substituted heteroaryls in Formula (I) or Formula (II) include, by way of example, pyrid-2-yl, pyrid-3-yl, pyrid-4-yl, fluoropyridyls (including 5-fluoropyrid-3-yl), chloropyridyls (including 5-chloropyrid-3-yl), thiophen-2-yl, thiophen-3-yl, benzothiazol-4-yl, 2-phenylbenzoxazol-5-yl, furan-2-yl, benzofuran-2-yl, thionaphthen-2-yl, 2-chlorothiophen-5-yl, 3-methylisoxazol-5-yl, 2-(thiophenyl)thiophen-5-yl, 6-methoxythionaphthen-2-yl, 3-phenyl-1,2,4-thiooxadiazol-5-yl, 2-phenyloxazol-4-yl, 5-chloro-1,3-dimethylpyrazol-4-yl; 2-methoxycarbonyl-thiophen-3-yl; 2,3-dimethylimidazol-5-yl; 2-methylcarbonylamino-4-methyl-thiazol-5-yl; quinolin-8-yl; thiophen-2-yl; 1-methylimidiazol-4-yl; 3,5-dimethylisoxazol-4-yl; and the like.  
      Particularly preferred R 1  groups include, by way of example only, 5-dimethylaminonaphth-1-yl, 2-chlorophenyl, 2-fluorophenyl, 2-bromophenyl, 2-hydroxyphenyl, 2-nitrophenyl, 2-methylphenyl, 2-methoxyphenyl,  
      R 1  may be also be sulfonated aminoalkyl such as Formula (V) below, wherein R 21  is hydrogen or methyl, and R 20  is an amino acid side chain or where R 20  and R 21  and the atoms to which they are attached form a heterocyclic or heteroaryl group of from 4 to 12 ring atoms, and R 22  is alkyl, substituted alkyl, aryl or substituted aryl.  
                 
 
      In one embodiment, R 1  is N-(4-methylbenzenesulfonyl)pyrol-2-yl, N-(4-chloro-2,5-dimethylbenzenesulfonyl)pyrol-2-yl, N-(napthylsulfonyl)pyrol-2-yl, N-(benzylsulfonyl)pyrol-2-yl; N-(4-chloro-2,5-dimethylbenzenesulfonyl)azetidin-2-yl, N-(4-chloro-2,5-dimethylbenzenesulfonyl)piperidin-2-yl, 1-(4-chloro-2,5-dimethylbenzenesulfonyl)-1,2,3,4-tetrahydroisoquinolin-2-yl, N-(4-chloro-2,5-dimethylbenzenesulfonyl)-N-methyl-aminomethyl; and 1-[N-(4-chloro-2,5-dimethylbenzenesulfonyl)amino]eth-1-yl; and the like.  
      R 22  is preferably selected from the group consisting of phenyl, 4-methylphenyl, 2,5-dimethylphenyl, 4-chlorophenyl, 2,5-dimethyl-4-chlorophenyl, benzyl, naphthyl, 1,2,3,4-tetrahydroisoquinoline, and the like.  
      R 20  is preferably hydrogen.  
      R 21  is preferably hydrogen, methyl, or ethyl.  
      Preferably R 20  and R 21  are joined to form a heterocyclic group, such as azetidinyl, pyrrolyl, piperidinyl, 1,2,3,4-tetrahydroisoquinolinyl, and the like.  
      Preferred R 2  groups include hydrogen, methyl, ethyl, isopropyl, 2-methoxyeth-1-yl, pyrid-3-ylmethyl, benzyl, t-butoxycarbonyl-methyl and the like. The particularly preferred R 2  is hydrogen.  
      Preferred R 3  groups include hydrogen, methyl, ethyl, isopropyl, 2-methoxyeth-1-yl, pyrid-3-ylmethyl, benzyl, t-butoxycarbonyl-methyl and the like. Particularly preferred R 3  groups include hydrogen, C 1-4 alkyl, optionally substituted monocyclic aryl, and optionally substituted monocyclic heteroaryl. Most preferred R 3  groups are hydrogen, methyl and phenyl.  
      Preferred R 4  groups include 4-(N,N-diethylamino)phenyl; 4-(N,N-dimethylamino)phenyl; 2-methylphenyl; phenyl; 1-naphthyl; 4-methylphenyl; 4-chlorophenyl; 3,4-dichlorophenyl; 4-methoxyphenyl; pyridin-3-yl; pyridin-4-yl; 2-chlorophenyl; 4-methoxy-3-hydroxyphenyl; 2-methoxypyridin-5-yl; 3,5-Dimethoxyphenyl; pyrazin-2-yl; 4-ethylphenyl; 4-(1-(RorS)-1-methylprop-1-yl); 1-methyl-1H-pyrazol-3-yl; 9H-fluoren-9-yl; isoquinolin-1-yl; isoquinolin-3-yl; 4-phenylthiazol-2-yl; 4-(4-pyridin-4-yl-piperazin-1-yl)phenyl; 4-[1,4′-bipiperidin]-1′-yl-phenyl; 1H-benzimidazol-2-yl; benzothiazol-2-yl; 4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl; 4-(3-aminopropyl)phenyl; 4-(2-aminoethyl)phenyl; 4-[1,4′-bipiperidin]-1′-yl-phenyl; 4-(2-(1,4,5,6-tetrahydropyrimidin-2-yl)ethyl)phenyl; 4-(4,5-dihydro-1H-imidazol-2-yl)phenyl; 4-fluoro-3-cyano-phenyl; and 4-(2-cyanoethyl)phenyl.  
      Additional preferred R 4  groups include 2-ethoxyphenyl; 3-(2-methylthiazol-5-yl)-pyrazol-5-yl, 4-aminophenylamino; 4-(1H-imidazol-2-ylmethyl)-phenylamino; 4-[2-(1H-imidazol-2-yl)-ethyl]-phenylamino; 4-aminomethyl-phenylamino; 4-(1H-imidazol-2-yl)-phenylamino; 4-[N,N′-diethylamidino]-phenylamino; 4-[N,N′-dimethylamidino]-phenylamino; 4-[N,N′-diphenylamidino]-phenylamino; 4-(4,5-dihydro-1H-imidazol-2-ylmethyl)-phenylamino; 4-(1H-benzimidazol-2-yl)-phenylamino; 4-[N-(4,5-dihydro-1H-imidazol-2-yl)aminomethyl]-phenylamino; 4-(1H-benzimidazol-2-ylmethyl)-phenylamino; 4-(1,4,5,6-tetrahydro-pyrimidin-2-yl)-phenylamino; and 4-(1,4,5,6-tetrahydro-pyrimidin-2-ylmethyl)-phenylamino.  
      Preferred R 5  groups include hydrogen, methyl, ethyl, iso-propyl, 2-methoxyethyl, and pyrid-3-yl-methyl.  
      Preferred X groups include hydrogen, bromine, chlorine, fluorine and methyl.  
      When R 3  in Formula (I) or Formula (II) is other than hydrogen, two geometric isomers may exist. When R 3  is hydrogen Formula (I) or Formula (II) are tautomers. In those cases where the compounds of Formula (I) or Formula (II) exist as tautomers, optical isomers or geometric isomers, the above formulas are intended to represent isomer mixtures as well as the individual isomeric bradykinin B 1  receptor antagonist or intermediate isomers, all of which are encompassed within the scope of this invention.  
      Further, references to the compounds of Formula (I) or Formula (II) with respect to pharmaceutical applications thereof are also intended to include pharmaceutically acceptable salts of the compounds of Formula (I) or Formula (II).  
      Compounds within the scope of this invention include those set forth in Table I as follows:  
               TABLE I                                                                                                  Cpd #   X 2     X   R 3     R 5     R 4                 201   Cl   Br   H   H   4-(N,N-diethylamino)phenyl       202   Cl   Br   H   H   4-(N,N-dimethylamino)phenyl       203   Cl   Br   H   H   2-methylphenyl       204   Cl   CH 3     H   H   phenyl       205   Cl   ethyl   H   H   phenyl       206   Cl   n-propyl   H   H   phenyl       207   Cl   Br   H   H   phenyl       209   Cl   Br   H   Me   phenyl       210   Cl   Br   H   H   1-naphthyl       211   Cl   Br   H   H   4-methylphenyl       212   Cl   Br   H   H   4-chlorophenyl       213   Cl   Br   H   H   3,4-dichlorophenyl       214   Cl   Br   H   H   4-methoxyphenyl       215   Cl   Br   H   H   pyridine-3-yl       216   Cl   Br   H   H   pyridine-4-yl       217   Cl   Br   H   H   2-chlorophenyl       218   Cl   Cl   H   H   phenyl       219   Cl   Br   H   H   4-methoxy-3-hydroxyphenyl       220   Cl   Br   H   H   2-methoxypyridin-5-yl       221   Cl   Br   H   H   3,5-Dimethoxyphenyl       222   Cl   Br   H   H   pyrazin-2-yl       224   Cl   Br   H   H   4-ethylphenyl       225   Cl   Br   H   H   4-(1-(R or S)-1-methylprop-1-yl)phenyl       226   Cl   Br   H   H   1-methyl-1H-pyrazol-3-yl       227   Cl   Br   H   H   9H-fluoren-9-yl       228   Cl   Br   H   H   isoquinolin-1-yl       229   Cl   Br   H   H   isoquinolin-3-yl       230   Cl   Br   H   H   4-phenylthiazol-2-yl       231   Cl   Br   H   H   4-(4-pyridin-4-yl-piperazin-1-yl)phenyl       232   Cl   Br   H   H   4-[1,4′-bipiperidin]-1′-yl-phenyl       233   Cl   Br   H   H   1H-benzimidazol-2-yl       234   Cl   Br   H   H   benzothiazol-2-yl       235   Cl   Br   H   H   4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl       236   Cl   Br   H   H   4-(3-aminopropyl)phenyl       237   Cl   Br   H   H   4-(2-aminoethyl)phenyl       238   F   Br   H   H   4-[1,4′-bipiperidin]-1′-yl-phenyl       239   Cl   Br   H   H   4-(2-(1,4,5,6-tetrahydropyrimidin-2-yl)ethyl)phenyl       240   Cl   Br   H   H   4-(4,5-dihydro-1H-imidazol-2-yl)phenyl       241   Cl   Br   H   H   4-fluoro-3-cyano-phenyl       242   Cl   Br   Me   H   4-[1,4′-bipiperidin]-1′-yl-phenyl       243   Cl   Br   Me   H   4-(4-pyridin-4-yl-piperazin-1-yl)phenyl       244   F   Me   t-butyl   H   4-(2-cyanoethyl)phenyl       245   F   Me   t-butyl   H   4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl       246   F   Me   H   H   4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl       247   F   Me   phenyl   H   4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl       248   F   Me   phenyl   H   4-(2-cyanoethyl)phenyl       249   Cl   Br   Me   H   4-(3-aminopropyl)phenyl       250   Cl   Br   Me   H   4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl                  
 
      Particularly preferred compounds of the present invention include the following: 
      4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-diethylaminophenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-dimethylaminophenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid o-tolylamide;     5-(2-chlorobenzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid phenylamide;     5-(2-chlorobenzoylamino)-4-ethyl-1H-pyrazole-3-carboxylic acid phenylamide;     5-(2-chlorobenzoylamino)-4-propyl-1H-pyrazole-3-carboxylic acid phenylamide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid phenylamide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid methylphenyl-amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid naphthalen-1-ylamide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid p-tolylamide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-chlorophenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (3,4-dichlorophenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-methoxyphenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid pyridin-3-ylamide     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid pyridin-4-ylamide     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (2-chlorophenyl)amide;     4-chloro-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid phenylamide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (3-hydroxy-4-methoxyphenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (6-methoxypyridin-3-yl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (3,5-dimethoxyphenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid pyrazin-2-ylamide;     4-bromo-5-(2-chlorobenzoylamino)-2H-pyrazole-3-carboxylic acid (4-ethylphenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-2H-pyrazole-3-carboxylic acid (4-sec-butylphenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (1-methyl-1H-pyrazol-3-yl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (9H-fluoren-9-yl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid isoquinolin-1-ylamide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid isoquinolin-3-ylamide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-phenylthiazol-2-yl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid [4-(4-pyridin-4-yl-piperazin-1-yl)phenyl]amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-[1,4′-bipiperidin]-1′-yl-phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (1H-benzimidazol-2-yl)amide; and     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid benzothiazol-2-ylamide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(3-aminopropyl)phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(2-aminoethyl)phenyl)amide;     4-bromo-5-(2-fluorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-[1,4′-bipiperidin]-1′-yl-phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(2-(1,4,5,6-tetrahydropyrimidin-2-yl)ethyl)phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(4,5-dihydro-1H-imidazol-2-yl)phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-fluoro-3-cyano-phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1-methyl-pyrazole-3-carboxylic acid (4-[1,4′-bipiperidin]-1′-yl-phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1-methyl-pyrazole-3-carboxylic acid [4-(4-pyridin-4-yl-piperazin-1-yl)phenyl]amide;     4-methyl-5-(2-fluorobenzoylamino)-1-t-butyl-pyrazole-3-carboxylic acid (4-(2-cyanoethyl)phenyl)amide;     4-methyl-5-(2-fluorobenzoylamino)-1-t-butyl-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide;     4-methyl-5-(2-fluorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide;     4-methyl-5-(2-fluorobenzoylamino)-1-phenyl-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide;     4-methyl-5-(2-fluorobenzoylamino)-1-phenyl-pyrazole-3-carboxylic acid (4-(2-cyanoethyl)phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1-methyl-pyrazole-3-carboxylic acid (4-(3-aminopropyl)phenyl)amide;     4-bromo-5-(2-chlorobenzoylamino)-1-methyl-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide; 
        and pharmaceutically acceptable salts thereof.    
       

      The following compounds are not included in the present invention  
                 
 
      In one preferred embodiment, in the compounds of Formula (I) and Formula (II), especially, but not necessarily, when R 3  is methyl and R 1  is methyl, then R 4  is other than substituted N-methylpyrazolyl.  
      Compounds of Formula (I) and Formula (II) can be employed as selective antagonists of the bradykinin B 1  over the bradykinin B 2  receptor.  
      The present invention also provides a selective antagonist of bradykinin B 1  receptor over bradykinin B 2  receptor that is a compound of Formula (I) or Formula (II).  
      This invention further provides a method for selectively inhibiting bradykinin B 1  receptor over bradykinin B 2  receptor in a biological sample comprising both the bradykinin B 1  and B 2  receptors which method comprises contacting an inhibiting effective amount of a compound of Formula (I) or Formula (II) or mixture thereof to the biological sample.  
      The present invention further provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an amount of a compound of Formula (I) or Formula (II) or mixtures thereof effective to treat or palliate adverse symptoms in mammals mediated by bradykinin B 1  receptor.  
      The present invention further provides a method for treating or palliating adverse symptoms in a mammal mediated at least in part by bradykinin B 1  receptor which method comprises administering a therapeutically effective amount of a compound of Formula (I) or Formula (II) or mixtures thereof or as is more generally the case the pharmaceutical composition.  
      The present invention further provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a therapeutically effective amount of a compound of Formula (I) or Formula (II) or mixtures thereof to treat or palliate adverse symptoms in a mammal associated with up-regulating bradykinin B 1  receptor following tissue damage or inflammation.  
      The present invention further provides a method for treating or palliating adverse symptoms in a mammal associated with tissue damage or inflammation which method comprises administering a therapeutically effective amount of a compound of Formula (I) or Formula (II) or mixtures thereof or as is more generally the case the pharmaceutical composition.  
      The present invention further provides a method for treating or palliating adverse symptoms associated with the presence or secretion of bradykinin B 1  receptor agonists in a mammal which method comprises administering a therapeutically effective amount of a compound of Formula (I) or Formula (II) or mixtures thereof or as is more generally the case the pharmaceutical composition.  
      The present invention provides a method for treating or ameliorating pain, inflammation, septic shock or the scarring process in a mammal mediated at least in part by bradykinin B 1  receptor which method comprises administering a therapeutically effective amount of a compound of Formula (I) or Formula (II) or mixtures thereof or as is more generally the case the pharmaceutical composition.  
      The present invention provides a method for treating or ameliorating adverse symptoms in a mammal associated with burns, perioperative pain, migraine, shock, central nervous system injury, asthma, rhinitis, premature labor, inflammatory arthritis, inflammatory bowel disease or neuropathic pain which method comprises administering a therapeutically effective amount of a compound of Formula (I) or Formula (II) or mixtures thereof or as is more generally the case the pharmaceutical composition.  
      The present invention further provides a method for treating or palliating adverse symptoms associated with the presence or secretion of bradykinin B 1  receptor agonists in a mammal which method comprises administering a therapeutically effective amount of a compound of Formula (I) or Formula (II) or mixtures thereof or as is more generally the case the pharmaceutical composition.  
      The invention also provides a method for determining bradykinin B 1  receptor agonist levels in a biological sample which method comprises contacting said biological sample with a compound of Formula (I) or Formula (II), at a predetermined concentration. 
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS  
      As noted above, this invention is directed to certain 3-amido-5-substituted pyrazole derivatives and related compounds which are useful as bradykinin B 1  receptor antagonists to relieve adverse symptoms in mammals mediated, at least in part, by bradykinin B 1  receptor including pain, inflammation, septic shock, the scarring process, etc. However, prior to describing this invention in further detail, the following terms will first be defined.  
      Definitions  
      Unless otherwise expressly defined with respect to a specific occurrence of the term, the following terms as used herein shall have the following meanings regardless of whether capitalized or not:  
      The term “alkyl” or “alk” refers to monovalent alkyl groups having from 1 to 15 carbon atoms and more preferably 1 to 6 carbon atoms and includes both straight chain and branched chain alkyl groups. This term is exemplified by groups such as methyl, t-butyl, n-heptyl, octyl and the like. The term C 1-4 alkyl refers to alkyl groups with from 1 to 4 carbon atoms.  
      The term “substituted alkyl” refers to an alkyl group, of from 1 to 15 carbon atoms, preferably, 1 to 6 carbon atoms, having from 1 to 5 substituents, preferably 1 to 3 substituents, independently selected from the group consisting of alkoxy, substituted alkoxy, acyl, acylamino, thiocarbonylamino, acyloxy, amino, substituted amino, amidino, alkylamidino, thioamidino, aminoacyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aryl, substituted aryl, aryloxy, substituted aryloxy, aryloxylaryl, substituted aryloxyaryl, cyano, halogen, hydroxyl, nitro, oxo, thioxo, carboxyl, carboxyl esters, cycloalkyl, substituted cycloalkyl, guanidino, guanidinosulfone, thiol, thioalkyl, substituted thioalkyl, thioaryl, substituted thioaryl, thiocycloalkyl, substituted thiocycloalkyl, thioheteroaryl, substituted thioheteroaryl, thioheterocyclic, substituted thioheterocyclic, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, cycloalkoxy, substituted cycloalkoxy, heteroaryloxy, substituted heteroaryloxy, heterocyclyloxy, substituted heterocyclyloxy, oxycarbonylamino, oxythiocarbonylamino, —OS(O) 2 -alkyl, —OS(O) 2 -substituted alkyl, —OS(O) 2 -aryl, —OS(O) 2 -substituted aryl, —OS(O) 2 -heteroaryl, —OS(O) 2 -substituted heteroaryl, —OS(O) 2 -heterocyclic, —OS(O) 2 -substituted heterocyclic, —OSO 2 —NR 40 R 40  where each R 40  is hydrogen or alkyl, —NR 40 S(O) 2 -alkyl, —NR 40 S(O) 2 -substituted alkyl, —NR 40 S(O) 2 -aryl, —NR 40 S(O) 2 -substituted aryl, —NR 40 S(O) 2 -heteroaryl, —NR 40 S(O) 2 -substituted heteroaryl, —NR 40 S(O) 2 -heterocyclic, —NR 40 S(O) 2 -substituted heterocyclic, —NR 40 S(O) 2 —NR 40 -alkyl, —NR 40 S(O) 2 —NR 40 -substituted alkyl, —NR 40 S(O) 2 —NR 40 -aryl, —NR 40 S(O) 2 —NR 40 -substituted aryl, —NR 40 S(O) 2 —NR 40 -heteroaryl, —NR 40 S(O) 2 —NR 40 -substituted heteroaryl, —NR 40 S(O) 2 —NR 40 -heterocyclic, and —NR 40 S(O) 2 —NR 40 -substituted heterocyclic where each R 40  is hydrogen or alkyl.  
      “Alkoxy” refers to the group “alkyl-O—” which includes, by way of example, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, tert-butoxy, sec-butoxy, n-pentoxy, n-hexoxy, 1,2-dimethylbutoxy, and the like.  
      “Substituted alkoxy” refers to the group “substituted alkyl-O—”.  
      “Acyl” refers to the groups H—C(O)—, alkyl-C(O)—, substituted alkyl-C(O)—, alkenyl-C(O)—, substituted alkenyl-C(O)—, alkynyl-C(O)—, substituted alkynyl-C(O)—, cycloalkyl-C(O)—, substituted cycloalkyl-C(O)—, aryl-C(O)—, substituted aryl-C(O)—, heteroaryl-C(O)—, substituted heteroaryl-C(O), heterocyclic-C(O)—, and substituted heterocyclic-C(O)— provided that a nitrogen atom of the heterocyclic or substituted heterocyclic is not bound to the —C(O)— group wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic are as defined herein.  
      “Amino” refers to the group —NH 2 .  
      “Substituted amino” refers to the group —NR 41 R 41 , where each R 41  group is independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, —SO 2 -alkyl, —SO 2 -substituted alkyl, —SO 2 -alkenyl, —SO 2 -substituted alkenyl, —SO 2 -cycloalkyl, —SO 2 -substituted cycloalkyl, —SO 2 -aryl, —SO 2 -substituted aryl, —SO 2 -heteroaryl, —SO 2 -substituted heteroaryl, —SO 2 -heterocyclic, —SO 2 -substituted heterocyclic, provided that both R 41  groups are not hydrogen; or the R 41  groups can be joined together with the nitrogen atom to form a heterocyclic or substituted heterocyclic ring.  
      The “acylamino” or as a prefix “carbamoyl” or “carboxamide” or “substituted carbamoyl” or “substituted carboxamide” refers to the group —C(O)NR 42 R 42  where each R 42  is independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic and where each R 42  is joined to form together with the nitrogen atom a heterocyclic or substituted heterocyclic wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic are as defined herein.  
      “Thiocarbonylamino” or as a prefix “thiocarbamoyl”, “thiocarboxamide” or “substituted thiocarbamoyl” or “substituted thiocarboxamide” refers to the group —C(S)NR 43 R 43  where each R 43  is independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic and where each R 43  is joined to form, together with the nitrogen atom a heterocyclic or substituted heterocyclic wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic are as defined herein.  
      “Acyloxy” refers to the groups acyl-O— where acyl is as defined herein.  
      “Alkenyl” refers to alkenyl group having from 2 to 10 carbon atoms and more preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-2 sites of alkenyl unsaturation.  
      “Substituted alkenyl” refers to alkenyl groups as defined herein, having from 1 to 5 substituents, preferably 1 to 3 substituents, independently selected from the group of substituents defined for substituted alkyl provided that the hydroxyl, thio, oxo or thioxo groups are not attached to a vinyl carbon atom.  
      “Alkynyl” refers to alkynyl group having from 2 to 10 carbon atoms and more preferably 3 to 6 carbon atoms and having at least 1 and preferably from 1-2 sites of alkynyl unsaturation.  
      “Substituted alkynyl” refers to alkynyl groups, as defined herein, having from 1 to 5, preferably 1 to 3 substituents, selected from the same group of substituents as defined for substituted alkyl provided that the hydroxyl, thio, oxo or thioxo groups are not attached to a vinyl carbon atom.  
      “Amidino” refers to the group H 2 NC(═NH)— and the term “alkylamidino” refers to compounds having 1 to 3 alkyl groups (e.g., alkylHNC(═NH)—).  
      “Thioamidino” refers to the group R 44 SC(═NH)— where R 44  is hydrogen or alkyl where alkyl is as defined herein.  
      “Aminoacyl” refers to the groups —NR 45 C(O)alkyl, —NR 45 C(O)substituted alkyl, —NR 45 C(O)cycloalkyl, —NR 45 C(O)substituted cycloalkyl, —NR 45 C(O)alkenyl, —NR 45 C(O)substituted alkenyl, —NR 45 C(O)alkynyl, —NR 45 C(O)substituted alkynyl, —NR 45 C(O)aryl, —NR 45 C(O)substituted aryl, —NR 45 C(O)heteroaryl, —NR 45 C(O)substituted heteroaryl, —NR 45 C(O)heterocyclic, and —NR 45 C(O)substituted heterocyclic where R 45  is hydrogen or alkyl and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic are defined herein.  
      “Aminocarbonyloxy” refers to the groups —NR 46 C(O)O-alkyl, —NR 46 C(O)O-substituted alkyl, —NR 46 C(O)O-alkenyl, —NR 46 C(O)O-substituted alkenyl, —NR 46 C(O)O-alkynyl, —NR 46 C(O)O-substituted alkynyl, —NR 46 C(O)O-cycloalkyl, —NR 46 C(O)O-substituted cycloalkyl, —NR 46 C(O)O-aryl, —NR 46 C(O)O-substituted aryl, —NR 46 C(O)O-heteroaryl, —NR 46 C(O)O-substituted heteroaryl, —NR 46 C(O)O-heterocyclic, and —NR 46 C(O)O-substituted heterocyclic where R 46  is hydrogen or alkyl and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic are as defined herein.  
      “Oxycarbonylamino” or as a prefix “carbamoyloxy” or “substituted carbamoyloxy” refers to the groups —OC(O)NR 47 R 47  where each R 47  is independently hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic or where each R 47  is joined to form, together with the nitrogen atom a heterocyclic or substituted heterocyclic and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic are as defined herein.  
      “Oxythiocarbonylamino” refers to the groups —OC(S)NR 48 R 48  where each R 48  is independently hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic or where each R 48  is joined to form, together with the nitrogen atom a heterocyclic or substituted heterocyclic and wherein alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic are as defined herein.  
      “Aminocarbonylamino” refers to the group —NR 49 C(O)NR 49 — where R 49  is selected from the group consisting of hydrogen and alkyl.  
      “Aminothiocarbonylamino” refers to the group —NR 50 C(S)NR 50 — where R 50  is selected from the group consisting of hydrogen and alkyl.  
      “Aryl” or “Ar” refers to an aromatic carbocyclic group of from 6 to 14 carbon atoms having a single ring (i.e., monocyclic) (e.g., phenyl) or multiple condensed rings (e.g., naphthyl or anthryl) which condensed rings may or may not be aromatic (e.g., 2-benzoxazolinone, 2H-1,4-benzoxazin-3(4H)-one, and the like). When at least one of the rings in the fused multicyclic ring system is non-aromatic, the point of attachment of the aryl group to the core structure is on one of the aromatic rings. Preferred aryls include phenyl and naphthyl.  
      “Substituted aryl” refers to aryl groups, as defined herein, which are substituted with from 1 to 4, preferably 1-3, substituents selected from the group consisting of hydroxy, acyl, acylamino, thiocarbonylamino, acyloxy, alkyl, substituted alkyl, alkoxy, substituted alkoxy, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, amidino, alkylamidino, thioamidino, amino, substituted amino, aminoacyl, aminocarbonyloxy, aminocarbonylamino, aminothiocarbonylamino, aryl, substituted aryl, aryloxy, substituted aryloxy, cycloalkoxy, substituted cycloalkoxy, heteroaryloxy, substituted heteroaryloxy, heterocyclyloxy, substituted heterocyclyloxy, carboxyl, carboxyl esters cyano, thiol, thioalkyl, substituted thioalkyl, thioaryl, substituted thioaryl, thioheteroaryl, substituted thioheteroaryl, thiocycloalkyl, substituted thiocycloalkyl, thioheterocyclic, substituted thioheterocyclic, cycloalkyl, substituted cycloalkyl, guanidino, guanidinosulfone, halo, nitro, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, oxycarbonylamino, oxythiocarbonylamino, —S(O) 2 -alkyl, —S(O) 2 -substituted alkyl, —S(O) 2 -cycloalkyl, —S(O) 2 -substituted cycloalkyl, —S(O) 2 -alkenyl, —S(O) 2 -substituted alkenyl, —S(O) 2 -aryl, —S(O) 2 -substituted aryl, —S(O) 2 -heteroaryl, —S(O) 2 -substituted heteroaryl, —S(O) 2 -heterocyclic, —S(O) 2 -substituted heterocyclic, —OS(O) 2 -alkyl, —OS(O) 2 -substituted alkyl, —OS(O) 2 -aryl, —OS(O) 2 -substituted aryl, —OS(O) 2 -heteroaryl, —OS(O) 2 -substituted heteroaryl, —OS(O) 2 -heterocyclic, —OS(O) 2 -substituted heterocyclic, —OSO 2 —NR 51 R 51  where each R 51  is hydrogen or alkyl, —NR 51 S(O) 2 -alkyl, —NR 51 S(O) 2 -substituted alkyl, —NR 51 S(O) 2 -aryl, —NR 51 S(O) 2 -substituted aryl, —NR 51 S(O) 2 -heteroaryl, —NR 51 S(O) 2 -substituted heteroaryl, —NR 51 S(O) 2 -heterocyclic, —NR 51 S(O) 2 -substituted heterocyclic, —NR 51 S(O) 2 —NR 51 -alkyl, —NR 51 S(O) 2 —NR 51 -substituted alkyl, —NR 51 S(O) 2 —NR 51 -aryl, —NR 51 S(O) 2 —NR 51 -substituted aryl, —NR 51 S(O) 2 —NR 51 -heteroaryl, —NR 51 S(O) 2 —NR 51 -substituted heteroaryl, —NR 51 S(O) 2 —NR 51 -heterocyclic, —NR 51 S(O) 2 —NR 51 -substituted heterocyclic where each R 51  is hydrogen or alkyl, wherein each of the terms is as defined herein.  
      “Aryloxy” refers to the group aryl-O— which includes, by way of example, phenoxy, naphthoxy, and the like.  
      “Substituted aryloxy” refers to substituted aryl-O— groups.  
      “Aryloxyaryl” refers to the group -aryl-O-aryl.  
      “Substituted aryloxyaryl” refers to aryloxyaryl groups substituted with from 1 to 4, preferably 1-3 substituents on either or both aryl rings independently selected from the same group consisting of substituents as defined for substituted aryl.  
      “Carboxyl” refers to the group —COOH and pharmaceutically acceptable salts thereof.  
      “Carboxyl esters” refer to any one of the following esters: —COO-alkyl, —COO-substituted alkyl, —COO-cycloalkyl, —COO-substituted cycloalkyl, —COO-aryl, —COO-substituted aryl, —COO-hetereoaryl, —COO-substituted heteroaryl, —COO-hetereocyclic, and —COO-substituted heterocyclic.  
      “Cycloalkyl” refers to cyclic alkyl groups of from 3 to 20 carbon atoms having a single or multiple cyclic rings including, by way of example, cyclopropyl, cyclobutyl, cyclopentyl, cyclooctyl, adamantanyl, and the like. Cycloalkyl groups of the present invention also include fused multicyclic rings wherein one or more of the rings within the multicyclic ring system are aryl, cycloalkenyl, heteroaryl, and/or heterocyclic, as long as the point of attachment to the core or backbone of the structure is on the non-aromatic cycloalkyl ring.  
      “Cycloalkenyl” refers to cyclic alkenyl groups of from 4 to 20 carbon atoms having single or multiple unsaturation and having a single or multiple cyclic unsaturated but not aromatic rings. Suitable cycloalkenyl groups include, by way of example, cyclopentenyl, cyclooctenyl, and the like. Cycloalkenyl groups of the present invention also include fused multicyclic rings wherein one or more of the rings within the multicyclic ring system are aryl, heteroaryl, cycloalkyl and/or heterocyclic, as long as the point of attachment to the core or backbone of the structure is on the non-aromatic cycloalkenyl ring.  
      “Substituted cycloalkyl” and “substituted cycloalkenyl” refer to a cycloalkyl and cycloalkenyl groups, as defined herein, having from 1 to 5, preferably 1-3 substituents independently selected from the same group of substituents as defined for substituted alkyl.  
      “Cycloalkoxy” refers to —O-cycloalkyl groups where cycloalkyl is as defined herein.  
      “Substituted cycloalkoxy” refers to —O-substituted cycloalkyl groups where substituted cycloalkyl is as defined herein.  
      “Guanidino” or “substituted guanidino” refers to the groups —NR 52 C(═NR 52 )NR 52 R 52  where each R 52  is independently hydrogen or alkyl.  
      “Guanidinosulfone” refers to the groups —NR 53 C(═NR 53 )NRSO 2 -alkyl, —NR 53 C(═NR 53 )NR 53 SO 2 -substituted alkyl, —NR 53 C(═NR 53 )NR 53 SO 2 -alkenyl, —NR 53 C(═NR 53 )NR 53 SO 2 -substituted alkenyl, —NR 53 C(═NR 53 )NR 53 SO 2 -alkynyl, —NR 53 C(═NR 53 )NR 53 SO 2 -substituted alkynyl, —NR 53 C(═NR 53 )NR 53 SO 2 -aryl, —NR 53 C(═NR 53 )NR 53 SO 2 -substituted aryl, —NR 53 C(═NR 53 )NR 53 SO 2 -cycloalkyl, —NR 53 C(═NR 53 )NR 53 SO 2 -substituted cycloalkyl, —NR 53 C(═NR 53 )NR 53 SO 2 -heteroaryl, —NR 53 C(═NR 53 )NR 53 SO 2 -substituted heteroaryl, —NR 53 C(═NR 53 )NR 53 SO 2 -heterocyclic, and —NR 53 C(═NR 53 )NR 53 SO 2 -substituted heterocyclic where each R 53  is independently hydrogen and alkyl.  
      “Halo” or “halogen” refers to fluoro, chloro, bromo and iodo.  
      “Heteroaryl” refers to an aromatic group of from 2 to 10 ring carbon atoms and 1 to 4 ring heteroatoms selected from oxygen, nitrogen and sulfur within the ring. Such heteroaryl groups can have a single ring (i.e., monocyclic) (e.g., pyridyl or furyl) or multiple condensed rings (e.g., indolizinyl or benzothienyl) which may be non-heteroaryl. When at least one of the rings in the fused multicyclic ring system is non-heteroaryl such as aryl, cycloalkyl, cycloalkenyl or heterocyclic, the point of attachment of the heteroaryl group to the core structure is on one of the heteroaryl atoms. Preferred heteroaryls include pyridyl, pyrrolyl, indolyl and furyl.  
      “Substituted heteroaryl” refers to heteroaryl groups, as defined above, which are substituted with from 1 to 3 substituents independently selected from the same group of substituents as defined for “substituted aryl”.  
      “Heteroaryloxy” refers to the group —O-heteroaryl and “substituted heteroaryloxy” refers to the group —O-substituted heteroaryl where heteroaryl and substituted heteroaryl are as defined above.  
      “Heterocycle” or “heterocyclic” refers to a saturated or unsaturated, but not heteroaromatic, group having a single ring or multiple condensed rings, from 2 to 20 ring carbon atoms and from 1 to 4 ring hetero atoms selected from nitrogen, sulfur or oxygen within the ring. “Heterocycle” or “heterocyclic” groups of the present invention also include fused multicyclic rings wherein one or more of the rings within the multicyclic ring system is not heterocyclic (e.g., cycloalkyl, cycloalkenyl, aryl or heteroaryl), as long as the point of attachment to the core or backbone of the structure is on the heterocyclic ring.  
      “Substituted heterocyclic” refers to heterocycle groups, as defined above, which are substituted with from 1 to 3 substituents independently selected from the group consisting of oxo (═O), thioxo (═S), plus the same group of substituents as defined for substituted aryl.  
      Examples of heterocycles and heteroaryls include, but are not limited to, azetidine, pyrrole, imidazole, pyrazole, pyridine, pyrazine, pyrimidine, pyridazine, indolizine, isoindole, indole, dihydroindole, indazole, purine, quinolizine, isoquinoline, quinoline, phthalazine, naphthylpyridine, quinoxaline, quinazoline, cinnoline, pteridine, carbazole, carboline, phenanthridine, acridine, phenanthroline, isothiazole, phenazine, isoxazole, phenoxazine, phenothiazine, imidazolidine, imidazoline, piperidine, piperazine, indoline, phthalimide, 1,2,3,4-tetrahydro-isoquinoline, 4,5,6,7-tetrahydrobenzo[b]thiophene, thiazole, thiazolidine, thiophene, benzo[b]thiophene, morpholino, thiomorpholino, piperidinyl, pyrrolidine, tetrahydrofuranyl, and the like.  
      “Heterocyclyloxy” refers to the group —O-heterocyclic and “substituted heterocyclyloxy” refers to the group —O-substituted heterocyclic where heterocyclic and substituted heterocyclyoxy are as defined above.  
      “Thiol” refers to the group —SH.  
      “Thioalkyl” or “thioalkoxy” refer to the groups —S-alkyl.  
      “Substituted thioalkyl” and “substituted thioalkoxy” refer to the group —S-substituted alkyl.  
      “Thiocycloalkyl” refers to the groups —S-cycloalkyl.  
      “Substituted thiocycloalkyl” refers to the group —S-substituted cycloalkyl.  
      “Thioaryl” refers to the group —S-aryl and “substituted thioaryl” refers to the group —S-substituted aryl.  
      “Thioheteroaryl” refers to the group —S-heteroaryl and “substituted thioheteroaryl” refers to the group —S-substituted heteroaryl.  
      “Thioheterocyclic” refers to the group —S-heterocyclic and “substituted thioheterocyclic” refers to the group —S-substituted heterocyclic.  
      Amino acid refers to any of the naturally occurring amino acids, as well as synthetic analogs (e.g., D-stereoisomers of the naturally occurring amino acids, such as D-threonine) and derivatives thereof. α-Amino acids comprise a carbon atom to which is bonded an amino group, a carboxyl group, a hydrogen atom, and a distinctive group referred to as a “side chain”. The side chains of naturally occurring amino acids are well known in the art and include, for example, hydrogen (e.g., as in glycine), alkyl (e.g., as in alanine, valine, leucine, isoleucine, proline), substituted alkyl (e.g., as in threonine, serine, methionine, cysteine, aspartic acid, asparagine, glutamic acid, glutamine, arginine, and lysine), arylalkyl (e.g., as in phenylalanine and tryptophan), substituted arylalkyl (e.g., as in tyrosine), and heteroarylalkyl (e.g., as in histidine). The term “naturally occurring amino acids” refers to these amino acids.  
      Unnatural amino acids are also known in the art, as set forth in, for example, Williams (ed.), Synthesis of Optically Active .alpha.-Amino Acids, Pergamon Press (1989); Evans et al., J. Amer. Chem. Soc., 112:4011-4030 (1990); Pu et al., J. Org Chem., 56:1280-1283 (1991); Williams et al., J. Amer. Chem. Soc., 113:9276-9286 (1991); and all references cited therein.  
      “Pharmaceutically acceptable salt” refers to pharmaceutically acceptable salts of a compound of Formula (I) or Formula (II) which salts are derived from a variety of organic and inorganic counter ions well known in the art and include, by way of example only, sodium, potassium, calcium, magnesium, ammonium, tetraalkylammonium, and the like; and when the molecule contains a basic functionality, salts of organic or inorganic acids, such as hydrochloride, hydrobromide, tartrate, mesylate, acetate, maleate, oxalate and the like.  
      The term “pharmaceutically acceptable prodrugs” refers to art recognized modifications to one or more functional groups which functional groups are metabolized in vivo to provide a compound of this invention or an active metabolite thereof. Such functional groups are well known in the art including acyl groups for hydroxyl and/or amino substitution, esters of mono-, di- and tri-phosphates wherein one or more of the pendent hydroxyl groups have been converted to an alkoxy, a substituted alkoxy, an aryloxy or a substituted aryloxy group, and the like.  
      It is understood that the substitution patterns defined herein do not include any chemically impossible substitution patterns. Moreover, when a group is defined by the term “substituted” such as substituted aryl and a possible substituent is the substituted group itself, e.g., substituted aryl substituted with substituted aryl, it is not intended that such substitution patterns be repeated indefinitely such as to produce a polymer, e.g., (substituted aryl) n . Accordingly, in all cases, the maximum number of repetitions is 4. That is too say that n is an integer from 1 to 4.  
      Compound Preparation  
      The compounds of this invention can be prepared from readily available starting materials using the following general methods and procedures. It will be appreciated that where typical or preferred process conditions (i.e., reaction temperatures, times, mole ratios of reactants, solvents, pressures, etc.) are given, other process conditions can also be used unless otherwise stated. Optimum reaction conditions may vary with the particular reactants or solvent used, but such conditions can be determined by one skilled in the art by routine optimization procedures.  
      Additionally, as will be apparent to those skilled in the art, conventional protecting groups may be necessary to prevent certain functional groups from undergoing undesired reactions. Suitable protecting groups for various functional groups as well as suitable conditions for protecting and deprotecting particular functional groups are well known in the art. For example, numerous protecting groups are described in T. W. Greene and G. M. Wuts,  Protecting Groups in Organic Synthesis , Third Edition, Wiley, New York, 1999, and references cited therein.  
      The compounds of this invention will typically contain one or more chiral centers. Accordingly, if desired, such compounds can be prepared or isolated as pure stereoisomers, i.e., as individual enantiomers or diastereomers, or as stereoisomer-enriched mixtures. All such stereoisomers (and enriched mixtures) are included within the scope of this invention, unless otherwise indicated. Pure stereoisomers (or enriched mixtures) may be prepared using, for example, optically active starting materials or stereoselective reagents well-known in the art. Alternatively, racemic mixtures of such compounds can be separated using, for example, chiral column chromatography, chiral resolving agents and the like.  
      The starting materials for the following reactions are generally known compounds or can be prepared by known procedures or obvious modifications thereof. For example, many of the starting materials are available from commercial suppliers such as Aldrich Chemical Co. (Milwaukee, Wis., USA), Bachem (Torrance, Calif., USA), Emka-Chemce or Sigma (St. Louis, Mo., USA). Others may be prepared by procedures, or obvious modifications thereof, described in standard reference texts such as Fieser and Fieser&#39;s Reagents for Organic Synthesis, Volumes 1-15 (John Wiley and Sons, 1991), Rodd&#39;s Chemistry of Carbon Compounds, Volumes 1-5 and Supplementals (Elsevier Science Publishers, 1989), Organic Reactions, Volumes 1-40 (John Wiley and Sons, 1991), March&#39;s Advanced Organic Chemistry, (John Wiley and Sons, 4 th  Edition), and Larock&#39;s Comprehensive Organic Transformations (VCH Publishers Inc., 1989).  
      Specifically, the substituted pyrazoles and various intermediates useful in the preparation of substituted pyrazoles are preferably prepared as shown in the following Schemes.  
                 
 
      Specifically, in Scheme 1 (where R 1 , R 3 , R 4 , R 5 , X, are as defined above and R* is alkyl), commercially available 3-nitro-5-carboxyl pyrazole, compound 117, is esterified under conventional conditions using a suitable alcohol, such as methanol, in acidic medium to provide for the corresponding ester, compound 118. The particular alcohol employed is not critical and is typically selected based on ease of synthesis and costs. The reaction is preferably conducted at an elevated temperature of from about 25 to about 100° C. until the reaction is substantially complete, which is typically 2 to 12 hours. The resulting product, compound 118, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      The 3-nitro-5-carboxyl ester pyrazole, compound 118, is protected with a conventional protecting group, Pg, under conventional conditions. The selected protecting group is one that is removed under conditions other than hydrogenation. A preferred protecting group is the Boc group.  
      The nitro group of the protected 3-nitro-5-carboxyl ester pyrazole, compound 119, is reduced to an amine using standard reduction reactions. For example, compound 119 is reacted with hydrogen gas at about 10 to 60 psi, in the presence of a suitable catalyst such as Pd on carbon to afford the corresponding amine, compound 120. The reaction is preferably conducted at a temperature of from about 20 to about 80° C. until the reaction is substantially complete, which is typically 1 to 5 hours. The resulting amine, compound 120, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      The 3-amino-5-carboxyl ester pyrazole, compound 120, is acylated under conventional conditions by reaction with at least a stoichiometric amount and preferably an excess of a desired acyl chloride, compound 121. The reaction is preferably conducted in the presence of a conventional activating agent such as DMAP in the presence of a base such as pyridine that scavenges the acid generated. The reaction is preferably conducted in an inert solvent such as dichloromethane, chloroform and the like although a liquid base such as pyridine can be employed as the solvent and to scavenge the acid generated. The reaction is preferably conducted at a temperature of from about −5 to about 35° C. until the reaction is substantially complete, which is typically 2 to 12 hours. The resulting product, compound 122, is obtained after a standard deprotection reaction, and can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Hydrolysis of compound 122, using conventional conditions such as lithium hydroxide and water in methanol and/or THF, affords the 3-(R 1 CO)-5-carboxylic acid pyrazole, compound 123.  
      Compound 123 is functionalized at the 4-position of the pyrazole ring by conventional methods to provide for compound 107. For example, when X is halo, compound 123 is contacted with at least a stoichiometric amount of a suitable halogenation agent such as N-halo succinimide, bromine, and the like. The reaction is conducted in an inert diluent such as dimethylformamide, dichloromethane, and the like at a temperature sufficient to effect halogenation. Typically, the reaction is conducted at from about 0° to about 40° C. until the reaction is substantially complete which typically occurs in about 0.1 to 10 hours. The resulting product, compound 107, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Subsequently, the carboxylic acid group of compound 107 is amidated using at least a stoichiometric amount and preferably an excess of a suitable amine, HNR 4 R 5 , under conventional conditions well known in the art preferably using an activating agent to effect coupling such as HOBT, EDC.HCL, NMM and the lice. The resulting compound 125 can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like.  
      Alternatively, compound 123 may be amidated as described above to form compound 124, which can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like. Compound 124 can be functionalized at the 4-position of the pyrazole ring by conventional methods to provide for compound 125 using the same methods described for the conversion of compound 123 to compound 107.  
      In an alternative synthetic embodiment, compounds of Formula (I) or Formula (II) where X is alkyl or hydrogen can be prepared as shown in Scheme 2 below:  
                 
 
      Specifically, in Scheme 2, wherein R 1  is defined above, commercially available oxalic acid diethyl ester, compound 145, is combined with at least a stoichiometric amount of an alkylnitrile, compound 146, in the presence of a suitable base such as potassium ethoxide in ethanol. The reaction is preferably maintained at a temperature of from about 60° C. to about 100° C. until the reaction is substantially complete, which is typically 12 to 24 hours. The resulting product, compound 147, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Compound 147 is then cyclized using a slight excess of t-butyl hydrazine hydrochloride (not shown) in ethanol. The reaction is preferably conducted at elevated temperatures and pressures such as a temperature of from about 75 to about 150° C. and a pressure of from about 1 to 10 atm until the reaction is substantially complete, which is typically 12 to 24 hours. The resulting product, compound 148, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Reaction of compound 148 proceeds in the manner described above for compound 120 to provide for compounds of Formula (I) or Formula (II) where X is alkyl.  
      Scheme 2 further illustrates derivatization of the carboxyl group of the 2-(Pg)-3-(—NHC(O)R 1 )-4-(X)-5-carboxyl pyrazole, compound 149. Specifically, conventional hydrolysis of the ester provides for compound 152 which is then converted to the methoxymethylamide by reaction with commercially available N—O-dimethyl-hydroxylamine hydrochloride under conventional coupling conditions in a suitable inert diluent such as tetrahydrofuran, dioxane, and the like optionally in the presence of an activating agent. The reaction is maintained under conditions sufficient to afford compound 153 including, for example, a temperature of from about 0 to about 40° C. for a period of from 12 to 24 hours. The resulting product, compound 153, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Compound 153 is then derivatized by contact with at least a stoichiometric amount, and preferably an excess, of Rˆ-Li where Rˆ is selected from the group consisting of alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, heterocycloalkyl, substituted heterocycloalkyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl. The reaction is typically conducted in an inert solvent such as tetrahydrofuran, dioxane, and the like at a reduced temperature of from about 0° C. to about −78° C. for a period of time sufficient for substantial reaction completion which typically occurs in about 12 to 24 hours. The resulting product, compound 155, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like  
      The starting materials employed in the reactions described above are either commercially available and/or can be prepared by methods well known in the art. For example, acid halides of the formula R 1 C(O)X are readily prepared from the corresponding carboxylic acid by reaction with, e.g., oxalyl halide, thionyl halide and the like. Acids of the formula R 1 C(O)OH are extremely well known and include aromatic acids (e.g., R 1  is aryl)  
      Alternatively, o-(Ar—S—CH 2 -)benzoyl chloride, compound 143, can be prepared as illustrated in Scheme 3 below where Ar is an aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic:  
                 
 
      Specifically, compound 140 is coupled to o-bromomethyl-benzoic acid methyl ester, compound 141 (prepared as per Dvornikovs  J. Org. Chem,  2002, 67, 2160), in the presence of about 30 equivalents potassium carbonate in DMF. This reaction is typically conducted at a temperature of from about 0 to about 30° C. until the reaction is substantially complete, which is typically 1 to 15 days. The resulting product, compound 142, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      o-(Ar—S—CH 2 -)benzoyl chloride, compound 143, is then prepared by conventional hydrolysis of the methyl ester in compound 142 followed by conventional conversion of the carboxyl group to the carboxyl halide using, e.g., oxalyl halide in the presence of a base to scavenge the acid generated. The reaction is typically conducted in an inert solvent such as dichloromethane. This reaction is typically run at a temperature of about −20 to about 10° C. until the reaction is substantially complete, which is typically 1 to 12 hours. The resulting product, compound 143, can be recovered by conventional methods, such as filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
                 
 
      In one embodiment, Z′ of the substituted pyrazoles of Formula 1 is sulfur. These substituted pyrazoles are prepared as shown in Scheme 4, where Pg, X, R 4 , R 5  and R 1  are as defined herein above.  
      Specifically, in Scheme 4, commercially available 3-nitro-5-carboxyl pyrazole, compound 117, is coupled to an amine using conventional conditions, for example, by using an activating agent such as HOBT, EDC.HCl, NMM and the like to effect coupling as described herein above. The resulting compound 108 can be recovered by conventional methods such as chromatography, filtration, crystallization and the like.  
      The 3-nitro-5-carboxylamide pyrazole, compound 108, is protected with a protecting group, Pg, under conventional conditions to afford compound 109. The selected protecting group is one that is removed under conditions other than hydrogenation. A preferred protecting group is the Boc group.  
      The nitro group of the protected 3-nitro-5-carboxylamide pyrazole, compound 109, is reduced to an amine using standard reduction reactions. For example, compound 109 is reacted with hydrogen gas at about 10 to 60 psi, in the presence of a suitable catalyst such as Pd on carbon to afford the corresponding amine, compound 110.  
      The 3-amino-5-carboxylamide pyrazole, compound 110, is converted to the thioamide, compound 111, under conventional conditions known in the art. Formation of thioamides from amides can be accomplished using a number of known methods including the use of P 4 S 10  or Lawesson&#39;s reagent as well as other methods know in the art such as those illustrated by Ernst Schaumann in  Comprehensive Organic Synthesis  Barry M. Trost, Ed.; Pergamon Press: Oxford, 1991; Vol. 6, Chapter 2.4, which is incorporated herein by reference in its entirety.  
      The 3-amino-5-thiocarboxyl amide pyrazole, compound 111, is acylated under conventional conditions by reaction with a desired acyl chloride, compound 121. The reaction is preferably conducted in the presence of a conventional activating agent such as DMAP in the presence of a base such as pyridine that scavenges the acid generated. The reaction is preferably conducted in an inert solvent such as dichloromethane, chloroform and the like. Alternatively a liquid base such as pyridine can be employed as the solvent and to scavenge the acid generated. The reaction is typically conducted at a temperature of about −5 to about 35° C. until completion, usually about 2 to about 12 hours. The resulting product, compound 112, is obtained after a standard deprotection reaction, and can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Compound 112, is functionalized at the 4-position of the pyrazole ring by conventional methods to provide for compound 113. For example, when X is halo, compound 112 is contacted with at least a stoichiometric amount of a suitable halogenation agent such as N-halo succinimide, Br 2 , and the like. The reaction is conducted in an inert diluent such as dimethylformamide, dichloromethane and the like at a temperature sufficient to effect halogenation. Typically, the reaction is conducted at from about 0 to about 40° C. until reaction is substantially complete which typically occurs in about 0.1 to 10 hours. The resulting product, compound 113, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
                 
 
      In one embodiment the substituted pyrazoles of Formula 1 in which Z′ is NH, the substituted pyrazoles are prepared as shown in Scheme 5.  
      Specifically, in Scheme 5,3-amino-5-cyano pyrazole, compound 161, is prepared by the addition of tert-butylhydrazine to fumaronitrile, compound 160. This reaction is typically run at a temperature of from about 0 to about 110° C. until substantially complete, usually about 1 to about 48 hours. The resulting product, compound 161 can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      The 3-amino-5-cyano pyrazole, compound 161, is acylated under conventional conditions by reaction with a desired acyl chloride, compound 121 to provide compound 162. The reaction is preferably conducted in the presence of a conventional activating agent such as DMAP in the presence of a base such as pyridine that scavenges the acid generated. The reaction is preferably conducted in an inert solvent such as dichloromethane, chloroform and the lice although a liquid base such as pyridine can be employed as the solvent and to scavenge the acid generated. The resulting product, compound 163, is obtained after a standard deprotection of compound 162, and can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Compound 163, is functionalized at the 4-position of the pyrazole ring by conventional methods to provide for compound 164. For example, when X is halo, compound 163 is contacted with at least a stoichiometric amount of a suitable halogenation agent such as N-halo succinimide, Bromine, and the like. The reaction is conducted in an inert diluent such as dimethylformamide, dichloromethane and the like at a temperature sufficient to effect halogenation. Typically, the reaction is conducted at from about 0 to about 40° C. until reaction is substantially complete which typically occurs in about 0.1 to 10 hours. The resulting product, compound 164, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Compound 164 is converted to the amidine, compound 165, under conventional conditions known in the art. Formation of amidines from nitriles can be accomplished using a number of known methods including condensation with amines. Other methods of preparing amidines are illustrated by Willi Kantlehner in  Comprehensive Organic Synthesis  Barry M. Trost, Ed.; Pergamon Press: Oxford, 1991; Vol. 6, Chapter 2.7.  
      Amines of the formula HNR 4 R 5  are well known in the art. One skilled in the art will understand how to obtain the aryl or heteroaryl amines used in the syntheses of the present invention. Briefly, aryl or heteroaryl amines can be obtained by reduction of the corresponding nitro compounds obtained by nitration of substituted aryl or heteroaryl rings or Curtius rearrangement of optionally substituted aryl or heteroaryl carboxylic acids. Many other aryl or heteroaryl amines are commercially available from chemical suppliers such as Aldrich, TCI and Lancaster Synthesis, for example.  
      R 1  may be a sulfonated aminoalkyl such as Formula (VI) below, wherein R 21  is hydrogen or methyl, and R 20  is an amino acid side chain or where R 20  and R 21  and the atoms to which they are attached form a heterocyclic or heteroaryl group of from 4 to 12 ring atoms, and R 22  is alkyl, substituted alkyl, aryl or substituted aryl.  
                 
 
      Compounds of Formula (I) or Formula (II) wherein R 1  is such a sulfonated amino group may be prepared as shown in Scheme 6 below where X, Z′, Q, R 2 , R 3 , R 20 , R 21  and R 22  are as defined herein.  
                 
 
      The amine group of compound 170 is converted to the sulfonamide using a suitable sulfonyl chloride, compound 175, and standard reactions conditions. For example, compound 170 may be reacted with an aryl sulfonyl chloride, compound 175, in the presence of a suitable base such as sodium carbonate an inert solvent such as water at a temperature of about 0° C. to about 100° C. until the reaction is substantially complete, typically 1 to about 24 hours. The product, compound 171, can be recovered by conventional methods, such as chromatography, filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or isolation.  
      Compound 171 is then converted to the acyl chloride using standard conditions. For example, compound 171 may be reacted with SOCl 2  in an inert solvent such as dichloromethane at a temperature of about −10° C. to about 39° C. until the reaction is substantially complete, typically 1 to about 24 hours. The product, compound 172, can be recovered by conventional methods, such as filtration, crystallization, and the like or, alternatively, used in the next step without purification and/or crystallization.  
      Compound 172 is then coupled to compound 110 to form compound 173, using well known methods which are described herein above for the amidation reactions in Scheme 1 (used to prepare compound 124 and/or compound 125). Compound 123 is functionalized at the 4-position of the pyrazole ring by conventional methods which are described herein above for the halogenation reactions in Scheme 1 (used to prepare compound 107 and/or 125) to afford compound 174.  
      The sulfonyl chlorides, compound 175, employed in the above reaction are either known compounds or compounds that can be prepared from known compounds by conventional synthetic procedures. Such compounds are typically prepared from the corresponding sulfonic acid, i.e., from compounds of the formula R 22 —SO 3 H where R 22  is as defined above, using phosphorous trichloride and phosphorous pentachloride. This reaction is generally conducted by contacting the sulfonic acid with about 2 to 5 molar equivalents of phosphorous trichloride and phosphorous pentachloride, either neat or in an inert solvent, such as dichloro-methane, at temperature in the range of about 0° C. to about 80° C. for about 1 to about 48 hours to afford the sulfonyl chloride. Alternatively, the sulfonyl chlorides can be prepared from the corresponding thiol compound, i.e., from compounds of the formula R 22 —SH where R 22  is as defined herein, by treating the thiol with chlorine (Cl 2 ) and water under conventional reaction conditions.  
      Pharmaceutical Formulations  
      When employed as pharmaceuticals, the compounds of Formula (I) or Formula (II) are usually administered in the form of pharmaceutical compositions. These compounds can be administered by a variety of routes including oral, rectal, transdermal, subcutaneous, intravenous, intramuscular, and intranasal. These compounds are effective as both injectable and oral compositions. Such compositions are prepared in a manner well known in the pharmaceutical art and comprise at least one active compound.  
      This invention also includes pharmaceutical compositions that contain, as the active ingredient, one or more of the compounds of Formula (I) or Formula (II) above associated with pharmaceutically acceptable carriers. In making the compositions of this invention, the active ingredient is usually mixed with an excipient, diluted by an excipient or enclosed within such a carrier which can be in the form of a capsule, sachet, paper or other container. When the excipient serves as a diluent, it can be a solid, semi-solid, or liquid material, which acts as a vehicle, carrier or medium for the active ingredient. Thus, the compositions can be in the form of tablets, pills, powders, lozenges, sachets, cachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols (as a solid or in a liquid medium), ointments containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions, and sterile packaged powders.  
      In preparing a formulation, it may be necessary to mill the active compound to provide the appropriate particle size prior to combining with the other ingredients. If the active compound is substantially insoluble, it ordinarily is milled to a particle size of less than 200 mesh. If the active compound is substantially water soluble, the particle size is normally adjusted by milling to provide a substantially uniform distribution in the formulation, e.g. about 40 mesh.  
      Some examples of suitable excipients include lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, and methyl cellulose. The formulations can additionally include: lubricating agents such as talc, magnesium stearate, and mineral oil; wetting agents; emulsifying and suspending agents; preserving agents such as methyl- and propylhydroxy-benzoates; sweetening agents; and flavoring agents. The compositions of the invention can be formulated so as to provide quick, sustained or delayed release of the active ingredient after administration to the patient by employing procedures known in the art.  
      The compositions are preferably formulated in a unit dosage form, each dosage containing 5 to about 100 mg, more usually about 10 to about 30 mg, of the active ingredient. The term “unit dosage forms” refers to physically discrete units suitable as unitary dosages for human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect, in association with a suitable pharmaceutical excipient.  
      The active compound is effective over a wide dosage range and is generally administered in a pharmaceutically effective amount. It, will be understood, however, that the amount of the compound actually administered will be determined by a physician, in the light of the relevant circumstances, including the condition to be treated, the chosen route of administration, the actual compound administered, the age, weight, and response of the individual patient, the severity of the patient&#39;s symptoms, and the like.  
      For preparing solid compositions such as tablets, the principal active ingredient is mixed with a pharmaceutical excipient to form a solid preformulation composition containing a homogeneous mixture of a compound of the present invention. When referring to these preformulation compositions as homogeneous, it is meant that the active ingredient is dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules. This solid preformulation is then subdivided into unit dosage forms of the type described above containing from, for example, 0.1 to about 500 mg of the active ingredient of the present invention.  
      The tablets or pills of the present invention may be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action. For example, the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an envelope over the former. The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permit the inner component to pass intact into the duodenum or to be delayed in release. A variety of materials can be used for such enteric layers or coatings, such materials including a number of polymeric acids and mixtures of polymeric acids with such materials as shellac, cetyl alcohol, and cellulose acetate.  
      The liquid forms in which the novel compositions of the present invention may be incorporated for administration orally or by injection include aqueous solutions suitably flavored syrups, aqueous or oil suspensions, and flavored emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil, or peanut oil, as well as elixirs and similar pharmaceutical vehicles.  
      Compositions for inhalation or insufflation include solutions and suspensions in pharmaceutically acceptable, aqueous or organic solvents, or mixtures thereof, and powders. The liquid or solid compositions may contain suitable pharmaceutically acceptable excipients as described supra. Preferably the compositions are administered by the oral or nasal respiratory route for local or systemic effect. Compositions in preferably pharmaceutically acceptable solvents may be nebulized by use of inert gases. Nebulized solutions may be breathed directly from the nebulizing device or the nebulizing device may be attached to a face masks tent, or intermittent positive pressure breathing machine. Solution, suspension, or powder compositions may be administered, preferably orally or nasally, from devices which deliver the formulation in an appropriate manner.  
      The following formulation examples illustrate the pharmaceutical compositions of the present invention.  
     FORMULATION EXAMPLE 1  
      Hard gelatin capsules containing the following ingredients are prepared:  
                                                       Quantity           Ingredient   (mg/capsule)                                                    Active Ingredient   30.0           Starch   305.0           Magnesium stearate   5.0                      
 
      The above ingredients are mixed and filled into hard gelatin capsules in 340 mg quantities.  
     FORMULATION EXAMPLE 2  
      A tablet formula is prepared using the ingredients below:  
                                                       Quantity           Ingredient   (mg/tablet)                                                    Active Ingredient   25.0           Cellulose, microcrystalline   200.0           Colloidal silicon dioxide   10.0           Stearic acid   5.0                      
 
      The components are blended and compressed to form tablets, each weighing 240 mg.  
     FORMULATION EXAMPLE 3  
      A dry powder inhaler formulation is prepared containing the following components:  
                                                   Ingredient   Weight %                                                    Active Ingredient   5           Lactose   95                      
 
      The active mixture is mixed with the lactose and the mixture is added to a dry powder inhaling appliance.  
     FORMULATION EXAMPLE 4  
      Tablets, each containing 30 mg of active ingredient, are prepared as follows:  
                                                       Quantity           Ingredient   (mg/tablet)                                                        Active Ingredient   30.0   mg           Starch   45.0   mg           Microcrystalline cellulose   35.0   mg           Polyvinylpyrrolidone   4.0   mg           (as 10% solution in water)           Sodium carboxymethyl starch   4.5   mg           Magnesium stearate   0.5   mg           Talc   1.0   mg           Total   120   mg                      
 
      The active ingredient, starch and cellulose are passed through a No. 20 mesh U.S. sieve and mixed thoroughly. The solution of polyvinyl-pyrrolidone is mixed with the resultant powders, which are then passed through a 16 mesh U.S. sieve. The granules so produced are dried at 50E to 60EC and passed through a 16 mesh U.S. sieve. The sodium carboxymethyl starch, magnesium stearate, and talc, previously passed through a No. 30 mesh U.S. sieve, are then added to the granules which, after mixing, are compressed on a tablet machine to yield tablets each weighing 150 mg.  
     FORMULATION EXAMPLE 5  
      Capsules, each containing 40 mg of medicament are made as follows:  
                                                       Quantity           Ingredient   (mg/capsule)                          Active Ingredient    40.0 mg           Starch   109.0 mg           Magnesium stearate    1.0 mg           Total   150.0 mg                      
 
      The active ingredient, cellulose, starch, an magnesium stearate are blended, passed through a No. 20 mesh U.S. sieve, and filled into hard gelatin capsules in 150 mg quantities.  
     FORMULATION EXAMPLE 6  
      Suppositories, each containing 25 mg of active ingredient are made as follows:  
                                                   Ingredient   Amount                          Active Ingredient     25 mg           Saturated fatty acid glycerides to   2,000 mg                      
 
      The active ingredient is passed through a No. 60 mesh U.S. sieve and suspended in the saturated fatty acid glycerides previously melted using the minimum heat necessary. The mixture is then poured into a suppository mold of nominal 2.0 g capacity and allowed to cool.  
     FORMULATION EXAMPLE 7  
      Suspensions, each containing 50 mg of medicament per 5.0 mL dose are made as follows:  
                                   Ingredient   Amount                                            Active Ingredient   50.0   mg       Xanthan gum   4.0   mg       Sodium carboxymethyl cellulose (11%)   50.0   mg       Microcrystalline cellulose (89%)       Sucrose   1.75   g       Sodium benzoate   10.0   mg                     Flavor and Color   q.v.           Purified water to 5.0 mL                  
 
      The medicament, sucrose and xanthan gum are blended, passed through a No. 10 mesh U.S. sieve, and then mixed with a previously made solution of the microcrystalline cellulose and sodium carboxymethyl cellulose in water. The sodium benzoate, flavor, and color are diluted with some of the water and added with stirring. Sufficient water is then added to produce the required volume.  
     FORMULATION EXAMPLE 8 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                   
               
               
                   
                   
                 Quantity 
               
               
                   
                 Ingredient 
                 (mg/capsule) 
               
               
                   
                   
               
             
            
               
                   
                 Active Ingredient 
                  15.0 mg 
               
               
                   
                 Starch 
                 407.0 mg 
               
               
                   
                 Magnesium stearate 
                  3.0 mg 
               
               
                   
                 Total 
                 425.0 mg 
               
               
                   
                   
               
            
           
         
       
     
      The active ingredient, cellulose, starch, and magnesium stearate are blended, passed through a No. 20 mesh U.S. sieve, and filled into hard gelatin capsules in 560 mg quantities.  
     FORMULATION EXAMPLE 9  
      An intravenous formulation may be prepared as follows:  
                                                   Ingredient   Quantity                                                        Active Ingredient   250.0   mg           Isotonic saline   1000   mL                      
 
     FORMULATION EXAMPLE 10  
      A topical formulation may be prepared as follows:  
                                                   Ingredient   Quantity                                                        Active Ingredient   1-10   g           Emulsifying Wax   30   g           Liquid Paraffin   20   g           White Soft Paraffin   to 100   g                      
 
      The white soft paraffin is heated until molten. The liquid paraffin and emulsifying wax are incorporated and stirred until dissolved. The active ingredient is added and stirring is continued until dispersed. The mixture is then cooled until solid.  
      Another preferred formulation employed in the methods of the present invention employs transdermal delivery devices (“patches”). Such transdermal patches may be used to provide continuous or discontinuous infusion of the compounds of the present invention in controlled amounts. The construction and use of transdermal patches for the delivery of pharmaceutical agents is well known in the art. See, e.g., U.S. Pat. No. 5,023,252, issued Jun. 11, 1991, which is incorporated herein by reference in its entirety. Such patches may be constructed for continuous, pulsatile, or on demand delivery of pharmaceutical agents.  
      When it is desirable or necessary to introduce the pharmaceutical composition to the brain, either direct or indirect techniques may be employed. Direct techniques usually involve placement of a drug delivery catheter into the host&#39;s ventricular system to bypass the blood-brain barrier. One such implantable delivery system used for the transport of biological factors to specific anatomical regions of the body is described in U.S. Pat. No. 5,011,472 which is incorporated herein by reference in its entirety.  
      Indirect techniques, which are generally preferred, usually involve formulating the compositions to provide for drug latentiation by the conversion of hydrophilic drugs into lipid-soluble drugs. Latentiation is generally achieved through blocking of the hydroxy, carbonyl, sulfate, and primary amine groups present on the drug to render the drug more lipid soluble and amenable to transportation across the blood-brain barrier. Alternatively, the delivery of hydrophilic drugs may be enhanced by intra-arterial infusion of hypertonic solutions which can transiently open the blood-brain barrier.  
      Utility  
      Bradykinin (“BK”) is a kinin that plays an important role in the patho-physiological processes accompanying acute and chronic pain and inflammation. Bradykinins, like other related kinins, are autocoid peptides produced by the catalytic action of kallikrein enzymes on plasma and tissue precursors termed kininogens. Inhibition of bradykinin B1 receptors by compounds that are bradykinin B1 antagonists or inverse agonists would provide relief from maladies that mediate undesirable symptoms through a BK B1 receptor pathway.  
      The compounds of this invention are the bradykinin B 1  receptor antagonists and therefore are suitable for use in blocking or ameliorating pain as well as hyperalgesia in mammals. Such compounds would be effective in the treatment or prevention of pain including, for example, bone and joint pain (osteoarthritis), repetitive motion pain, dental pain, cancer pain, myofascial pain (muscular injury, fibromyalgia), perioperative pain (general surgery, gynecological) and chronic pain. In particular, inflammatory pain such as, for example, inflammatory airways disease (chronic obstructive pulmonary disease) would be effectively treated by bradykinin B1 antagonist compounds.  
      The compounds of this invention are also useful in the treatment of disease conditions in a mammal that are mediated, at least in part, by bradykinin B 1  receptor. Examples of such disease conditions include asthma, inflammatory bowel disease, rhinitis, pancreatitis, cystitis (interstitial cystitis), uveitis, inflammatory skin disorders, rheumatoid arthritis and edema resulting from trauma associated with burns, sprains or fracture. They may be used subsequent to surgical intervention (e.g. as post-operative analgesics) and to treat inflammatory pain of varied origins (e.g. osteoarthritis, rheumatoid arthritis, rheumatic disease, tenosynovitis and gout) as well as for the treatment of pain associated with angina, menstruation of cancer. They may be used to treat diabetic vasculopathy, post capillary resistance or diabetic symptoms associated with insulitis (e.g. hyperglycemia, diuresis, proteinuria and increased nitrite and kallikrein urinary excretion). They may be used as smooth muscle relaxants for the treatment of spasm of the gastrointestinal tract or uterus or in the therapy of Crohn&#39;s disease, ulcerative colitis or pancreatitis. Such compounds may be used therapeutically to treat hyperreactive airways and to treat inflammatory events associated with airways disease e.g. asthma, and to control, restrict or reverse airways hyperreactivity in asthma. They may be used to treat intrinsic and extrinsic asthma including allergic asthma (atopic or non-atopic) as well as exercise-induced asthma, occupational asthma, asthma post-bacterial infection, other non-allergic asthmas and “wheezy-infant syndrome”. They may also be effective against pneumoconiosis, including aluminosis, anthracosis, asbestosis, chalicosis, ptilosis, siderosis, silicosis, tabacosis and byssinosis was well as adult respiratory distress syndrome, chronic obstructive pulmonary or airways disease, bronchitis, allergic rhinitis, and vasomotor rhinitis. Additionally, they may be effective against liver disease, multiple sclerosis, atherosclerosis, Alzheimer&#39;s disease, septic shock e.g. as anti-hypovolemic and/or anti-hypotensive agents, cerebral edema, headache, migraine, closed head trauma, irritable bowel syndrome and nephritis. Finally, such compounds are also useful as research tools (in vivo and in vitro).  
      As noted above, the compounds of this invention are typically administered to the mammal in the form of a pharmaceutical composition. Pharmaceutical compositions of the invention are suitable for use in a variety of drug delivery systems. Suitable formulations for use in the present invention are found in  Remington&#39;s Pharmaceutical Sciences , Mace Publishing Company, Philadelphia, Pa., 17th ed. (1985).  
      In order to enhance serum half-life, the compounds may be encapsulated, introduced into the lumen of liposomes, prepared as a colloid, or other conventional techniques may be employed which provide an extended serum half-life of the compounds. A variety of methods are available for preparing liposomes, as described in, e.g., Szoka, et al., U.S. Pat. No. 4,235,871, Geho, et al., U.S. Pat. No. 4,501,728 and Allen, et al., U.S. Pat. No. 4,837,028 each of which is incorporated herein by reference.  
      The amount administered to the patient will vary depending upon what is being administered, the purpose of the administration, such as prophylaxis or therapy, the state of the patient, the manner of administration, and the like all of which are within the skill of the attending clinician. In therapeutic applications, compositions are administered to a patient already suffering from a disease in an amount sufficient to cure or at least partially arrest the symptoms of the disease and its complications. An amount adequate to accomplish this is defined as “therapeutically effective dose.” Amounts effective for this use will depend on the disease condition being treated as well as by the judgment of the attending clinician depending upon factors such as the severity of the inflammation, the age, weight and general condition of the patient, and the like.  
      The compositions administered to a patient are in the form of pharmaceutical compositions described above. These compositions may be sterilized by conventional sterilization techniques, or may be sterile filtered. The resulting aqueous solutions may be packaged for use as is, or lyophilized, the lyophilized preparation being combined with a sterile aqueous carrier prior to administration. The pH of the compound preparations typically will be between 3 and 11, more preferably from 5 to 9 and most preferably from 7 to 8. It will be understood that use of certain of the foregoing excipients, carriers, or stabilizers will result in the formation of pharmaceutical salts.  
      The therapeutic dosage of the compounds of the present invention will vary according to, for example, the particular use for which the treatment is made, the manner of administration of the compound, the health and condition of the patient, and the judgment of the prescribing physician. For example, for intravenous administration, the dose will typically be in the range of about 20 μg to about 500 μg per kilogram body weight, preferably about 100 μg to about 300 μg per kilogram body weight. Effective doses can be extrapolated from dose-response curves derived from in vitro or animal model test systems.  
      Alternatively, about 0.1 mg/day to about 1,000 mg/day of a compound, or mixture of compounds, of the present invention may be administered orally, preferably from about 1 mg/day to about 100 mg/day, and more preferably from 5 mg/day to about 50 mg/day. From about 0.5 to about 100 mg/day may be given to a patient for parenteral, sublingual, intranasal or intrathecal administration; for depo administration and implants, from about 0.5 mg/day to about 50 mg/day; for topical administration from about 0.5 mg/day to about 200 mg/day; for rectal administration from about 0.5 mg to about 500 mg; and more preferably for parenteral administration, from about 5 to about 50 mg daily.  
      The following synthetic and biological examples are offered to illustrate this invention and are not to be construed in any way as limiting the scope of this invention. Unless otherwise stated, all temperatures are in degrees Celsius.  
     EXAMPLES  
      In the examples below, the following abbreviations have the following meanings. If an abbreviation is not defined, it has its generally accepted meaning. 
          Ac=Acetyl     aq.=aqueous     Boc=t-butoxycarbonyl     bs=broad singlet     d=doublet     dd=doublet of doublets     DMAP=4-N,N-dimethylaminopyridine     DMF=N,N-dimethylformamide     EDC or EDC.HCL=1-(3-dimethylaminopropyl)-3-ethylcarbo-diimidehydrochloride     Et=ethyl     EtOH=ethanol     eq.=equivalents     g=gram     HATU=O-(7-azabenzotriazol-1-yl)-1,1,3-,3-tetramethyl-uronium hexafluorophosphate     HOBT=1-hydroxybenzothiazole hydrate     Hz=hertz     HPLC=high performance liquid chromatography     MS=mass spectroscopy     Me=methyl     MeOH=methanol     m=multiplet     M=molar     mg=milligram     min.=minutes     mL=milliliter     MHz=Megahertz     MP=meso porous     N=Normal     NBC=N-bromosuccinamide     NMR=nuclear magnetic resonance     NMM=N-methylmorpholine     OAc=acetate     PS=polystyrene     psi=pounds per square inch     q=quartet     rt=room temperature     s=singlet     sat.=saturated     t=triplet     THF=tetrahydrofuran     wt/wt=weight to weight ratio     μL=microliters        

      In the following examples and procedures, the term “Aldrich” indicates that the compound or reagent used in the procedure is commercially available from Aldrich Chemical Company, Inc., Milwaukee, Wis. 53233 USA; the term “Sigma” indicates that the compound or reagent is commercially available from Sigma, St. Louis Mo. 63178 USA; the term “TCI” indicates that the compound or reagent is commercially available from TCI America, Portland Oreg. 97203; the term “Frontier” or “Frontier Scientific” indicates that the compound or reagent is commercially available from Frontier Scientific, Utah, USA; the term “Bachem” indicates that the compound or reagent is commercially available from Bachem, Torrance, Calif., USA. The term “Matrix” indicates that the compound or reagent is commercially available from Matrix Scientific, Columbia, S.C., USA. The term “Ambinter” indicates that the compound or reagent is commercially available from Ambinter Paris, France.  
      The following general procedures illustrate general synthetic pathways for preparing 3-amido-5-substituted pyrazole derivatives of Formula (I) or Formula (II) and amine intermediates useful in preparing the same.  
     General Procedure 1  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (7)  
     
       
         
         
             
             
         
       
     
      Preparation of 3-Methoxycarbonyl-5-nitropyrazole (18). A solution of 5-nitro-1H-pyrazole-3-carboxylic acid (17, Aldrich, cat. no. 41,483-2) in MeOH was prepared. While stirring, HCl was bubbled through the solution for 2 min. The reaction mixture was refluxed for a time sufficient for complete esterification and allowed to cool to rt. The solvent was removed by rotary evaporation. The crude material was basified by addition of saturated aqueous NaHCO 3  and extracting with EtOAc. The combined organic extracts were dried over MgSO 4  and filtered. The filtrate was rotary evaporated and dried under vacuum to yield 18.  
      Preparation of 1-tert-Butoxycarbonyl-3-methoxycarbonyl-5-nitropyrazole (19). A solution of 1.0 eq. of 18, 1.1 eq. of (Boc) 2 O, , 1.5 eq. of Et 3 N, and 0.05 eq. of DMAP in CH 2 Cl 2  was prepared. The reaction mixture was stirred at rt for a time sufficient for reaction completion and the solvent was removed by rotary evaporation. The crude material was dried under vacuum to afford product 19.  
      Preparation of 5-Amino-1-tert-butoxycarbonyl-3-methoxycarbonylpyrazole (20). A mixture of 1.0 eq. of 19 and 0.1 wt/wt eq. of 10% Pd on carbon was hydrogenated at 10-60 psi of hydrogen for a time sufficient for reaction completion. The reaction mixture was filtered through Celite. The filtrate was concentrated by rotary evaporation. The crude material was dried under vacuum to give product 20.  
      Preparation of 5-(2-Chloro-benzoylamino)-pyrazole-1,3-dicarboxylic acid 1-tert-butyl ester 3-methyl ester (28). A solution of 1.0 eq. of 20, 1.5 eq. of pyridine, and 0.04 eq. of DMAP in CH2Cl2 was prepared. After cooling to 0° C., 1.1 eq. of 2-chlorobenzoyl chloride (Aldrich, cat. no. 10,391-8) was added. The reaction solution was allowed to warm to rt and after a time sufficient for reaction completion, the solvent was removed by rotary evaporation to afford crude 28.  
      Preparation of 5-(2-Chlorobenzoylamino)-3-methoxycarbonylpyrazole (22). A solution of 28 in 1 M HCl was stirred for 5 min and extracted with EtOAc. The combined organic extracts were washed with saturated aqueous NaHCO3, followed by drying over MgSO4 and filtering. The filtrate was rotary evaporated and dried under vacuum to yield 22.  
      Preparation of 5-(2-Chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (23). A solution of 1.0 eq. of 22 and 5.0 eq. of LiOH.H 2 O in THF, MeOH, and H 2 O (2:1:1) was stirred at rt. After a time sufficient for reaction completion, the reaction mixture was rotary evaporated. The mixture was acidified with concentrated HCl. As the pH of the solution reached about 2, a precipitate formed. The solid was collected by filtration and after drying under vacuum, product 23 was obtained.  
      Preparation of the title compound (7). A solution of 1.0 eq. of 23 in DMF was prepared. While stirring, a solution of 1.2 eq. of N-bromosuccinamide in DMF was added. After stirring at rt for a time sufficient for reaction completion, H 2 O was added. The mixture was extracted with EtOAc. The combined organic extracts were washed with 1 M HCl, followed by drying over MgSO 4  and filtering. The filtrate was rotary evaporated. The crude material was triturated with CH 2 Cl 2  and dried under vacuum to yield 7.  
     General Procedure 2  
     Preparation of 4-Chloro-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (24)  
     
       
         
         
             
             
         
       
     
      A solution of 1.0 eq. of 23 in DMF was prepared. While stirring, 1.3 eq. of N-chlorosuccinamide and a small amount of concentrated HCl were added. After stirring at rt for a time sufficient for reaction completion, H 2 O was added. The quenched reaction solution was extracted with EtOAc. The combined organic extracts were dried over MgSO 4  and filtered. The filtrate was rotary evaporated. The crude material was triturated with CH 2 Cl 2  and dried under vacuum to yield 24.  
     General Procedure 3  
     Preparation of (R)-4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid amides (25)  
     
       
         
         
             
             
         
       
     
      Compound 7 was prepared as shown in General Procedure 1. Compound 25 was prepared as shown in General Procedure 3. A mixture of 1.0 eq. of 7, 1.1 eq. of 2, 1.2 eq. of HOBT, 2.2 eq. of NMM, and 1.2 eq. of EDC.HCl in THF was stirred at rt. After a time sufficient for reaction completion, the reaction mixture was adsorbed onto silica gel and flash chromatographed using a mixture of EtOAc/hexanes as eluant to give product 25.  
     General Procedure 4  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid anilinoamides (27)  
     
       
         
         
             
             
         
       
     
      A solution of 1.0 eq. of 7 and 1.3 eq. of 26 in dry pyridine was cooled to −10° C. While stirring, 1.1 eq. of POCl 3  was added dropwise. The cooling bath was removed after 10 min and the mixture was stirred at rt. After 10 min, 1.0 M HCl was added. The mixture was extracted with EtOAc. The combined organic extracts were washed with saturated aqueous NaHCO 3 , followed by drying over MgSO 4  and filtering. The filtrate was rotary evaporated. The crude material was flash chromatographed using a mixture of EtOAc-hexanes as eluant to yield 27.  
     General Procedure 5  
     Preparation of 5-[(2-Chloro-benzoyl)-methyl-amino]-1H-pyrazole-3-carboxylic acid methyl ester (29)  
     
       
         
         
             
             
         
       
     
      A suspension of 1.0 eq. of ester 28, prepared in General Procedure 1, in THF was stirred at −78° C. as 2.0 eq. of a 2.5 M solution of n-BuLi in THF was added. The cooling bath was removed and the reaction mixture was allowed to stir while warming for 10 min. The mixture was cooled back to −78° C. and 2.0 eq. of MeI was added. The bath was again removed and the reaction mixture was allowed to warm to rt. After a time sufficient for reaction completion, the reaction was quenched with 1 M HCl and extracted with EtOAc. The organic layer was washed with sat. aq. NaHCO 3 , dried over MgSO 4 , filtered and the solvent removed by rotary evaporation. The material was purified by flash chromatography on silica gel using a mixture of EtOAc-hexanes as eluant to afford 29.  
     General Procedure 6  
     Preparation of 4-Bromo-5-[2-(quinolin-8-ylthiomethyl)benzoylamino]-1H-pyrazole-3-carboxylic acid (43)  
     
       
         
         
             
             
         
       
     
      Preparation of 2-(Quinolin-8-ylthiomethyl)benzoic acid methyl ester (41). A solution of 4.0 eq. of quinoline-8-thiol hydrochloride (39, Aldrich, cat. no. 35,978-5) was dissolved in DMF. To this was added 32.0 eq. of potassium carbonate. The mixture was stirred at room temperature for 20 minutes and 1.0 eq of 2-bromomethyl-benzoic acid methyl ester (40 , J. Org. Chem,  2002, 67, 2160) was added. The mixture was stirred at room temperature for a time sufficient enough for reaction completion. The mixture was diluted with 0.1 M citric acid and extracted with EtOAc. The organic layer was washed with brine and dried with Na 2 SO 4 , filtered, and concentrated. The product was purified by flash chromatography on silica gel using a mixture of EtOAc-hexanes as eluant to give 41.  
      Preparation of 2-(Quinolin-8-ylthiomethyl)benzoyl chloride (42). A solution of 1.0 eq of ester 41 and 3.0 eq. of LiOH in methanol and water was heated to 65° C. for a time sufficient for completion of the hydrolysis. The mixture was cooled to room temperature and concentrated, then diluted with H 2 O. The pH of the aqueous mixture was adjusted to 4.5 and extracted with EtOAc. The organic layer was washed with brine and dried over Na 2 SO 4 , filtered, and concentrated to give the intermediate benzoic acid.  
      A solution of 1.0 eq. of 2-(quinolin-8-ylthiomethyl)benzoic acid in CH 2 Cl 2  was cooled to 0° C. To this was added 1.1 eq. of oxalyl chloride followed by one drop of DMF. The mixture was warmed to room temperature and stirred for a time sufficient for reaction completion. The mixture was concentrated to give 42 which was used directly.  
      Preparation of the title compound (43). The procedure described for compound 22 was employed using 2-(quinolin-8-ylthiomethyl)benzoyl chloride (42). Hydrolysis of the methyl ester as described for compound 23 afforded acid 43.  
     General Procedure 7  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid amides (44)  
     
       
         
         
             
             
         
       
     
      A solution of 1.0 eq. of acid 7, 1.0 eq. of amine 2, and 8.1 eq. of Et 3 N in DMF was prepared. While stirring, a solution of 1.0 eq. of HATU dissolved in DMF was added. After stirring at rt for a time sufficient for reaction completion, 6.0 eq. of MP-carbonate resin and 6.0 eq. of PS-trisamine resin (both from Argonaut Technologies, Inc.) were added. The mixture was stirred at rt for 16 hrs, filtered, and washed with DMF and MeOH. The crude material was purified by reverse-phase HPLC using a mixture of acetonitrile-water as eluant. The purified material was concentrated and dried to afford amide 44.  
     General Procedure 8  
     Preparation of 5-(2-Chloro-benzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid (51)  
     
       
         
         
             
             
         
       
     
      Preparation of Potassium 2-cyano-1-ethoxycarbonyl-2-methylethenolate (47). Placed potassium ethoxide (1.0 eq.) in a sealed tube with EtOH and shook until dissolved. A mixture of diethyl oxalate (1.0 eq.) and propionitrile (1.0 eq.) was added to the sealed tube and the mixture was capped and stirred at reflux. After a time sufficient for reaction completion, the reaction was cooled and the precipitate collected and washed with diethyl ether to afford 47.  
      Preparation of 5-Amino-1-tert-butyl-4-methyl-1H-pyrazole-3-carboxylic acid ethyl ester (48). Placed 47 (1.0 eq.) into a sealed pressure reaction flask. Added EtOH and t-butylhydrazine hydrochloride (1.1 eq.). The pressure flask was capped and heated to reflux. After a time sufficient for reaction completion, the mixture was evaporated to dryness and the solid obtained was dissolved in equal amounts of EtOAc and water. The organic layer was washed with saturated aqueous NaHCO 3 , brine, dried with MgSO 4 , and evaporated. This slightly yellow solid was triturated with hexanes and filtered to afford ester 48.  
      Preparation of 1-tert-Butyl-5-(2-chloro-benzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid ethyl ester (49). The procedure described for compound 22 was employed using 5-amino-1-tert-butyl-4-methyl-1H-pyrazole-3-carboxylic acid ethyl ester (49).  
      Preparation of 5-(2-Chloro-benzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid ethyl ester (50). Ester 48 was dissolved in a minimal amount of formic acid and heated to 80° C. for a time sufficient for reaction completion. Formic acid was removed via rotary evaporation to yield 50.  
      Preparation of the title compound (51). The procedure described for compound 23 was employed using 5-(2-chloro-benzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid ethyl ester (50).  
     General Procedure 9  
     Preparation of N-(5-Carboxyalkyl-4-methyl-2H-pyrazol-3-yl)-2-chloro-benzamide (55)  
     
       
         
         
             
             
         
       
     
      Preparation of 1-tert-Butyl-5-(2-chloro-benzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid (52). The procedure described for compound 23 was employed using 1-tert-butyl-5-(2-chlorobenzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid ethyl ester (49) to afford acid 52.  
      Preparation of 1-tert-Butyl-5-(2-chlorobenzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid methoxymethylamide (53). The procedure described for compound 3 was employed using 1-tert-butyl-5-(2-chloro-benzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid (52) and N,O-dimethylhydroxylamine hydrochloride (Aldrich, cat. no. D16,370-8).  
      Preparation of N-(5-Carboxyalkyl-2-tert-butyl-4-methyl-2H-pyrazol-3-yl)-2-chlorobenzamides (54). To a flask equipped with a stirbar was added 53 (1.0 eq.) dissolved in THF under a nitrogen atmosphere. The mixture was cooled to −10° C. and a 1.4 M solution of MeLi (6.0 eq.) in diethyl ether was added dropwise. The mixture was allowed to slowly warm to room temperature and stirred for a time sufficient for reaction completion. The reaction was poured into 0.1 N HCl and extracted with dichloromethane, dried over MgSO 4 , filtered and concentrated to a crude oil. The crude material was purified by column chromatography eluting with a mixture of EtOAc-hexanes to afford 54.  
      Preparation of the title compound (55). The procedure described for compound 50 was employed using benzamide 54.  
     General Procedure 10  
     Preparation of 4-Bromo-5-(2-m-tolylthiomethylbenzoylamino)-1H-pyrazole-3-carboxylic acid (62)  
     
       
         
         
             
             
         
       
     
      Preparation of 2-m-Tolylthiomethyl-benzoyl chloride (59). A solution of 1.0 eq. of 58 ( Coll. Czech. Chem. Comm.  1982, 47, 3094) in CH 2 Cl 2  was prepared. While stirring, 1.1 eq. of oxalyl chloride and one drop of DMF was added. After stirring at rt for a time sufficient for reaction completion, the reaction mixture was rotary evaporated and dried under vacuum to produce 59.  
      Preparation of 5-(2-m-Tolylthiomethybenzoylamino)-1H-pyrazole-3-carboxylic acid methyl ester (60). A solution of 1.1 eq. of 20, 1.1 eq. of pyridine, and 0.07 eq. of DMAP in CH 2 Cl 2  was cooled to 0° C. While stirring, a solution of 1.0 eq. of 59 in CH 2 Cl 2  was added. The reaction solution was allowed to warm to rt. After a time sufficient for reaction completion, the reaction solution was concentrated by rotary evaporation and 1.0 M HCl was added. The acidified solution was extracted with EtOAc. The combined organic extracts were washed with saturated aqueous NaHCO 3 , followed by drying over MgSO 4  and filtering. The filtrate was rotary evaporated and the crude material was purified by flash chromatography on silica gel using a mixture of EtOAc-hexanes as eluant to give 60.  
      Preparation of 4-Bromo-5-(2-m-tolylthiomethylbenzoylamino)-1H-pyrazole-3-carboxylic acid methyl ester (61). A solution of 1.0 eq. of 60 in DMF was prepared. While stirring, a solution of 1.1 eq. of NBS in DMF was added. After stirring at rt for a time sufficient for reaction completion, water was added. The solution was extracted with EtOAc. The combined organic extracts dried over MgSO 4  and vacuum filtered. The filtrate was rotary evaporated and the crude material was purified by flash chromatography on silica gel using a mixture of EtOAc-hexanes as eluant to give 61.  
      Preparation of the title compound (62). The procedure described for compound 23 was employed with methyl ester 61 to afford acid 62.  
     General Procedure 11  
     Preparation of 3-(4-Amino-phenyl)-propionitrile (66)  
     
       
         
         
             
             
         
       
     
      Preparation of p-Toluene-4-sulfonic acid 2-(4-nitrophenyl)ethyl ester (64). To a solution of 1.0 eq. of 2-(4-nitrophenyl)ethanol 63 (Aldrich, 18,346-6) and 1.1 eq. triethylamine in CH 2 Cl 2  was added p-toluenesulfonyl chloride (1.0 eq.). The mixture was stirred overnight under nitrogen and concentrated to dryness. The crude solid was taken up in equal amounts of EtOAc and sat. aq. copper sulfate solution. The organic layer was washed with brine and dried over Na 2 SO 4 . Removing the solvent followed by trituration with Et 2 O afforded compound 64 as a slightly tan solid.  
      Preparation of 3-(4-nitrophenyl)propionitrile (65). To a solution of sodium cyanide (1.3 eq.) in DMSO (5 ml) pre-heated to 70° C. was added 1.0 eq. of p-toluene-4-sulfonic acid 2-(4-nitrophenyl)ethyl ester (64). Upon completion of addition, the reaction mixture was heated to over 90° C. and stirred for 2 hours. The brown solution was cooled to rt and water was added to give a white precipitate which was extracted into CH 2 Cl 2 . The extracts were then washed with water and dried over Na 2 SO 4 . Column chromatography with gradients of EtOAc/hexanes quickly removed baseline impurities and afforded a light orange oil which was sufficiently pure for further elaboration.  
      Preparation of 3-(4-Aminophenyl)propionitrile (66). 3-(4-Nitrophenyl)propionitrile, compound (65), was dissolved into EtOAc and placed in a Parr hydrogenation bottle. A spatula tip of 5% Pd/C sat. with H 2 O) was added to the bottle and the mixture placed on Parr hydrogenation shaker at 30 psi for approximately 3 hours. The reaction was filtered through Celite, dried over Na 2 SO 4  to afford compound 66.  
     General Procedure 13  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid {4-[2-(4,5-dihydro-1H-imidazol-2-yl)ethyl]phenyl}amide (68)  
     
       
         
         
             
             
         
       
     
      HCl gas was bubbled for 10 minutes into a solution of 67 (prepared as shown in General Procedure 4 using compound 66 and pyrazole acid 7) in EtOH and cooled to 0° C. (1.0 eq.). After 30 minutes of stirring a white precipitate formed. The mixture was stirred for an additional 5 hours and then evaporated to dryness. The crude mixture was then dissolved in EtOH and ethylene diamine (1.1 eq.) was added. The reaction mixture was then stirred under nitrogen overnight. The mixture was evaporated to dryness and purified by preparative HPLC to afford compound 68 as the trifluoroacetate salt.  
     General Procedure 14  
     Preparation of [3-(4-Aminophenyl)propyl]carbamic acid tert-butyl ester (70)  
     
       
         
         
             
             
         
       
     
      Preparation of 4-(3-Aminopropyl)phenylamine (69). Nitrile 65 (8 g, 45 mmol) was dissolved in absolute ethanol (500 mL) under argon. Palladium on carbon (10%, 0.8 g) was added followed by hydrazine monohydrate (6.6 mL, 136 mmol). After 2 hr, the catalyst was filtered off and Raney nickel (8 g) was added followed by more hydrazine monohydrate (6.6 mL, 136 mmol) and the reaction mixture was heated under argon to 53° C. After 1 hr, a further 6.6 mL hydrazine monohydrate was added and the mixture was heated for a further 2 hr. Removal of the catalyst gave diamine 69 as an oil which was used without further purification.  
      Preparation of [3-(4-Aminophenyl)propyl]carbamic acid tert-butyl ester (70). Diamine 69 (6.6 g, 44 mmol) was dissolved in anhydrous CH 2 Cl 2  (150 mL) and cooled in an ice bath. Di-tert-butyl-dicarbonate (9.59 g, 44 mmol) was added as a solution (100 mL anhydrous CH 2 Cl 2 ) slowly while the bath was maintained at 0° C. After 2 hr at 0° C., the solution was washed with sat. aq. NaHCO 3  (100 mL), brine (100 mL) and dried over Na 2 SO 4 . The crude product was purified by column chromatography (silica, 30-50% EtOAc:hexanes) to give aniline 70 as a yellow solid.  
     General Procedure 15  
     Preparation of 5-Amino-1-methyl-1H-pyrazole-3-carboxylic acid methyl ester (74) and 5-Amino-2-methyl-2H-pyrazole-3-carboxylic acid methyl ester (73)  
     
       
         
         
             
             
         
       
     
      Preparation of 1-Methyl-5-nitro-1H-pyrazole-3-carboxylic acid methyl ester (71) and 2-Methyl-5-nitro-2H-pyrazole-3-carboxylic acid methyl ester (72). A suspension of 60% (weight) NaH dispension in mineral oil (10.9 g, 273 mmol) was added in portions into a stirred solution of 5-nitro-1H-pyrazole-3-carboxylic acid methyl ester (18) (18.6 g, 109 mmol) in anhydrous THF (200 mL) cooled under an ice-water bath. After stirring for 35 min, methyl iodide (20.4 mL, 327 mmol) was added, and the reaction mixture was stirred for 20 hr. The solvent was evaporated and the residue was taken up in EtOAc (200 mL), washed with water (60 mL), and stirred over anhydrous MgSO 4  for 20 min. After filtration and concentration, a colorless oil (22.2 g) was obtained, which was confirmed by HPLC/MS and NMR analyses as a mixture of 71 and 72 (see: Baraldi, Pier Giovanni, et al;  Molecules  [Electronic Publication], 1998, 3(2), M46) in a 1:2.27 ratio.  
      Preparation of 5-Amino-1-methyl-1H-pyrazole-3-carboxylic acid methyl ester (73) and 5-Amino-2-methyl-2H-pyrazole-3-carboxylic acid methyl ester (74). A solution of a mixture of methyl esters 71 and 72 was dissolved in ethanol (60 mL), 10% Pd/C (1.0 g) was added, and the mixture was hydrogenated at 30 psi of H 2  for 16 hr. The mixture was filtered through a layer of Celite and evaporated to afford a yellow solid (16.0 g), which was indicated by HPLC-MS analysis to be a mixture of 73 and 74. The two isomers were separated by flash chromatography (1:1 EtOAc/hexanes) to yield 74 (9.90 g, 63.9 mmol, 59%) (Ho H. Lee, et al;  J. Org. Chem.  1989, 54,428-431) and 73 (4.14 g, 26.7 mmol, 24.5%) (Ho H. Lee, et al;  J. Org. Chem.  1989, 54, 428-431).  
       1 H-NMR (74) (CDCl 3 ) δ 6.13 (s, 1H), 4.00 (s, 3H), 3.85 (s, 3H), 3.62 (br, 2H).  
       1 H-NMR (73) (CDCl 3 ) δ 6.06 (s, 1H), 3.86 (s, 3H), 3.72 (s, 3H), 3.66 (br, 2H).  
     General Procedure 16  
     Preparation of 5-Amino-1-phenyl-1H-pyrazole-3-carboxylic acid ethyl ester (76)  
     
       
         
         
             
             
         
       
     
      A suspension of 1.0 eq. of 3-cyano-2-oxopropanoic acid ethyl ester (75) (Degussa, NACOPE) and 1.2 eq. of phenylhydrazine hydrochloride in absolute EtOH was stirred at reflux for 3 days. The reaction mixture was cooled to rt and filtered through Celite. The solvent was removed by rotary evaporation. Purification of the material on silica gel using 50% EtOAc-hexanes as eluant afforded compound 76.  
     Example 1  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (4-diethylamino-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with N,N-diethyl-1,4-phenylenediamine (Aldrich, 26,151-3) using the method described in General Procedure 3.  
      MS + (m/z) = 490.0  
       1 H-NMR (CDCl 3 ) δ 12.51 (br, 1H), 9.19 (s, 1H), 8.76 (s, 1H), 8.02 (d, J=7.5 Hz, 1H), 7.55-7.41 (m, 5H), 6.64 (d, J=9.0 Hz, 2H), 3.33 (q, J=7.0 Hz, 4H), 1.15 (t, J=7.0 Hz, 6H).  
     Example 2  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (4-dimethylamino-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with N,N-dimethyl-1,4-phenylenediamine (Aldrich, 19,399-2) using the method described in General Procedure 3.  
      MS+=462.1  
       1 H-NMR (DMSO-d6) δ 7.61-7.46 (m, 6H), 6.72 (d, J=8.9 Hz, 2H), 2.87 (s, 6H).  
     Example 3  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid o-tolylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with o-toluidine (Aldrich, 18,542-6) using the method described in General Procedure 3.  
      MS+=433.0  
       1 H-NMR (CDCl3) δ 12.68 (br, 1H), 9.32 (s, 1H), 8.92 (s, 1H), 8.04 (d, J=8.0 Hz, 1H), 7.91 (d, J=7.6 Hz, 1H), 7.53 (m, 2H), 7.38 (m, 1H), 7.23 (m, 2H), 7.13 (m, 1H), 2.35 (s, 3H).  
     Example 4  
     Preparation of 5-(2-Chloro-benzoylamino)-4-methyl-1H-pyrazole-3-carboxylic acid phenylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid prepared as described in General Procedure 8 was coupled to aniline (Aldrich, 24,228-4 using the method described in General Procedure 3.  
      MS+=355.0  
       1 H-NMR (DMSO-d6) δ 7.82 (d, J=7.7 Hz, 2H), 7.63-7.30 (m, 6H), 7.07 (t, J=7.7 Hz, 1H), 2.20 (s, 3H).  
     Example 5  
     Preparation of 5-(2-Chloro-benzoylamino)-4-ethyl-1H-pyrazole-3-carboxylic acid phenylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid prepared from diethyl oxylate (45) and butyronitrile following the method of General Procedure 8 was coupled with aniline (Aldrich, 24,228-4) using the method of General Procedure 3.  
      MS+=369.1  
       1 H-NMR (CD3OD) δ 7.72-7.34 (m, 8H), 7.14 (d, J=7.3 Hz, 1H), 2.83 (q, J=7.5 Hz, 2H), 1.21 (t, J=6.6 Hz, 3H).  
     Example 6  
     Preparation of 5-(2-Chloro-benzoylamino)-4-propyl-1H-pyrazole-3-carboxylic acid phenylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid prepared from diethyl oxylate (45) and valeronitrile (Aldrich, 15,509-8) following the method of General Procedure 8 was coupled with aniline (Aldrich, 24,228-4) using the method of General Procedure 3.  
      MS+=383.0  
       1 H-NMR (DMSO-d6) δ 13.40 (br s, 1H), 10.54 (br s, 1H), 9.95 (br s, 1H), 7.79 (d, J=7.9 Hz, 2H), 7.61-7.47 (m, 4H), 7.32 (t, J=7.7 Hz, 2H), 7.07 (t, J=7.3 Hz, 1H), 2.66 (t, J=7.2 Hz, 2H), 1.52 (m, 2H), 0.86 (t, J=7.2 Hz, 3H).  
     Example 7  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid phenylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with aniline (Aldrich, 24,228-4) using the method described in General Procedure 3.  
      MS+=419.0  
       1 H-NMR (DMSO-d6) δ 10.83 (br, 1H), 10.20 (br, 1H), 7.77 (m, 2H), 7.54 (m, 4H), 7.34 (m, 2H), 7.10 (m, 1H).  
     Example 8  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid methyl-phenyl-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with N-methylaniline (Aldrich, 23,623-3) using the method described in General Procedure 3.  
      MS+=434.9  
       1 H-NMR (CDCl3) δ 11.88 (br, 1H), 8.99 (br, 1H), 7.92 (d, J=7.7 Hz, 1H), 7.47 (m, 2H), 7.41 (m, 1H), 7.23 (m, 5H), 3.49 (s, 3H).  
     Example 9  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid naphthalen-1-ylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 1-naphthylamine (Fluka, 70731) using the method described in General Procedure 3.  
      MS+=469.0  
       1 H-NMR (DMSO-d6) δ 10.86 (br, 1H), 10.26 (br, 1H), 7.74 (m, 11H).  
     Example 10  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid p-tolylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with p-toluidine (Aldrich, 23,631-4) using the method described in General Procedure 3.  
      MS+=433.0  
     Example 11  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (4-chloro-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 4-chloroaniline (Aldrich, 47,722-2) using the method described in General Procedure 3.  
      MS+=452.9  
     Example 12  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (3,4-dichloro-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 3,4-dichloroaniline (Aldrich, 43,777-8) using the method described in General Procedure 3.  
      MS+=486.9  
     Example 13  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (4-methoxy-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with p-Anisidine (Fluka, 10490) using the method described in General Procedure 3.  
      MS+=448.9  
     Example 14  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid pyridin-3-ylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 2-Aminopyridine (Acros, 40106) using the method described in General Procedure 3.  
      MS+=419.9  
     Example 15  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid pyridin-4-ylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 4-aminopyridine (Fluka, 09370) using the method described in General Procedure 3.  
      MS+=419.9  
     Example 16  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (2-chloro-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 2-chloroaniline (Fluka, 23300) using the method described in General Procedure 3.  
      MS+=452.9  
     Example 17  
     Preparation of 4-Chloro-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid phenylamide  
     
       
         
         
             
             
         
       
     
      The title compound was prepared as shown in General Procedure 2 followed by coupling to aniline (Aldrich, 24,228-4) using the method described in General Procedure 3.  
      MS+=375.0  
       1 H-NMR (CD3OD) δ 7.86 (m, 1H), 7.48 (m, 7H), 7.15 (m, 1H).  
     Example 18  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (3-hydroxy-4-methoxy-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 5-Amino-2-methoxyphenol (Acros Organics, 33491) using the methods described in General Procedure 4.  
      MS+=465.0  
       1 H-NMR (CD 3 OD) δ 7.64 (m, 1H), 7.48 (m, 3H), 7.26 (m, 1H), 7.09 (m, 1H), 6.91 (m, 1H), 3.86 (m, 3H).  
     Example 19  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (6-methoxy-pyridin-3-yl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 5-amino-2-methoxypyridine (Pfaltz &amp; Bauer Chemicals, A24130) using the method of General Procedure 4.  
      MS+=450.0  
       1 H-NMR (CD 3 OD) δ 8.46 (m, 1H), 8.01 (m, 1H), 7.64 (m, 1H), 7.50 (m, 3H), 6.82 (d, J=9.3 Hz, 1H), 3.91 (s, 3H).  
     Example 20  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (3,5-dimethoxy-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 3,5-dimethoxyaniline (Fluka, 38600) using the method of General Procedure 7.  
      MS+=479.0  
     Example 21  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid pyrazin-2-ylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with aminopyrazine (Fluka, 09332) using the method of General Procedure 7.  
      MS+=442.2 (M+Na)  
     No Example 22  
     Example 23  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-2H-pyrazole-3-carboxylic acid (4-ethyl-phenyl) -amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 4-ethylaniline (Fluka, 03070) using the method of General Procedure 7.  
      MS+=447.0  
     Example 24  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino-2H-pyrazole-3-carboxylic acid (4-sec-butyl-phenyl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 4-sec-butylaniline (Fluka, 19559) using the method of General Procedure 7.  
      MS+=475.1  
     Example 25  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (1-methyl-1H-pyrazol-3-yl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared using the methods described in General Procedure 1 followed by coupling to 1-methyl-1H-pyrazol-3-amine (TimTec, Inc., TBB019586) using the method of General Procedure 3.  
      MS+423.0  
       1 H-NMR (CD 3 OD) δ 7.61 (m, 1H), 7.51 (m, 4H), 6.66 (m, 1H), 3.85 (s, 3H).  
     Example 26  
     Preparation of 4-Bromo-5-(2-chloro-benzoylamino)-1H-pyrazole-3-carboxylic acid (9H-fluoren-9-yl)-amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled with 9-aminofluorene hydrochloride (Acros, 26928) using the method described in General Procedure 3.  
      MS+507.0  
       1 H-NMR (DMSO-d6) δ 8.75 (d, J=8.3 Hz, 1H), 7.87 (d, J=7.4 Hz, 2H), 7.58-7.31 (m, 13H), 6.14 (m, 1H).  
     Example 27  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid isoquinolin-1-ylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared using the methods described in General Procedure 1 followed by coupling to 1-aminoisoquinoline (Aldrich, 17,859-4) using the method of General Procedure 3.  
      MS+=469.8  
       1 H-NMR (CDCl3) δ 8.08 (m, 2H), 7.81 (m, 4H), 7.66 (m, 2H), 7.53 (m, 2H).  
     Example 28  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid isoquinolin-3-ylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared using the methods described in General Procedure 1 followed by coupling to 3-isoquinolinamine (Ryan Scientific, BTB 10019) using the method of General Procedure 3.  
      MS+=469.8  
       1 H-NMR (DMSO-d6) δ 8.47 (d, J=6.7 Hz, 1H), 8.39 (d, J=6.8 Hz, 1H), 7.98 (d, J=6.1 Hz, 1H), 7.88 (d, J=6.3 Hz, 1H), 7.76 (m, 1H), 7.58 (m, 5H).  
     Example 29  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-phenylthiazol-2-ylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared using the methods described in General Procedure 1 followed by coupling to 2-amino-4-phenylthiazole (Alfa, A18488) using the method of General Procedure 3.  
      MS+=502.0  
       1 H-NMR (DMSO-d6) δ 7.59 (m, 9H).  
     Example 30  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid [4-(4-pyridin-4-yl-piperazin-1-yl)phenyl]amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared using the methods described in General Procedure 1 followed by coupling to 4-(4-pyridin-4-yl-piperazin-1-yl)phenylamine (WO 02/099388) using the method of General Procedure 3.  
      MS+=580.0  
       1 H-NMR (DMSO-d6) δ 14.05 (br, 1H), 13.55 (br, 1H), 10.08 (br, 1H), 8.28 (d, J=4.8 Hz, 2H), 7.61 (m, 6H), 7.26 (d, J=4.7 Hz, 2H), 7.01 (d, J=6.1 Hz, 2H), 3.87 (m, 4H), 3.33 (m, 4H).  
     Example 31  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-[1,4′-bipiperidin]-1′-yl-phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared using the methods described in General Procedure 1 followed by coupling to 4-[1,4′-bipiperidin]-1′-yl-phenylamine (WO 02/099388) using the method of General Procedure 3.  
      MS+=585.1  
       1 H-NMR (DMSO-d6) δ 9.94 (br, 1H), 7.56 (m, 6H), 6.93 (d, J=6.4 Hz, 2H), 4.10 (m, 2H), 3.70 (m, 2H), 2.60 (m, 4H), 2.36 (m, 1H), 1.81 (m, 2H), 1.55 (m, 6H), 1.40 (m, 2H).  
     Example 32  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (1H-benzimidazol-2-yl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared using the methods described in General Procedure 1 followed by coupling to 2-aminobenzimidazole (Aldrich, 17,177-8) using the method of General Procedure 3.  
      MS+=458.9  
     Example 33  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid benzothiazol-2-ylamide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared using the methods described in General Procedure 1 followed by coupling to 2-aminobenzothiazole (Aldrich, 10,881-2) using the method of General Procedure 3.  
      MS+=475.9  
     Example 34  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino-1H-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The title compound was prepared as described in General Procedure 13.  
      MS+=517.0  
       1 H-NMR (CD 3 OD) δ 7.67 (m, 2H), 7.55 (m, 4H), 7.26 (m, 2H), 3.99 (br m, 4H), 3.00 (br m, 2H), 2.85 (br m, 2H).  
     Example 35  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(3-aminopropyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid 7, prepared as described in Procedure 1 was coupled with aniline 70, prepared as described in Procedure 14, using the method of Procedure 4 followed by treatment with TFA.  
      MS+=476.0  
       1 H-NMR (DMSO-d6) δ 7.62 (m, 6H), 7.18 (m, 2H), 2.76 (m, 2H), 2.60 (m, 2H), 1.81 (m, 2H).  
     Example 36  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(2-aminoethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid 7, prepared as described in Procedure 1 was coupled with [2-(4-aminophenyl)ethyl]carbamic acid tert-butyl ester (J &amp; W PharmLab LLC, 20-0111) using the method of Procedure 4 followed by treatment with TFA.  
      MS+=462.1  
       1 H-NMR (DMSO-d6) δ 14.10 (br, 1H), 10.84 (br, 1H), 10.20 (br, 1H), 7.78 (m, 3H), 7.59 (m, 4H), 7.23 (m, 2H), 3.04 (m, 2H), 2.83 (m, 2H).  
     Example 37  
     Preparation of 4-bromo-5-(2-fluorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-[1,4′-bipiperidin]-1′-yl-phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared as described for compound 22 using amine 20 and 2-fluorobenzoyl chloride (Aldrich, 12,084-7) followed by bromination as shown in General Procedure 8. The amine is prepared as described in International patent application WO 02/099388, and coupled the pyrazole acid as shown in General Procedure 4.  
      MS+=569.2  
       1 H-NMR (DMSO-d6) δ 10.06 (br, 1H), 7.83 (m, 1H), 7.70 (m, 3H), 7.48 (m, 2H), 7.01 (m, 2H), 4.12 (m, 2H), 2.69 (m, 2H), 2.59 (m, 4H), 2.43 (m, 1H), 1.88 (m, 2H), 1.47 (m, 6H), 1.27 (m, 2H).  
     Example 38  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(2-(1,4,5,6-tetrahydropyrimidin-2-yl)ethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The title compound was prepared as shown in General Procedure 13 using propylene diamine.  
      MS+=531.1  
       1 H-NMR (CD3OD) δ 7.68 (d, 2H, J=8.7 Hz), 7.53 (m, 2H), 7.46 (m, 2H), 7.24 (d, 2H, J=8.7 Hz), 3.37 (m, 4H), 2.97 (t, 2H, J=7.2 Hz and 6.6 Hz), 2.69 (t, 2H, J=6.6 and 7.2 Hz), 1.92 (m, 2H).  
     Example 39  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(4,5-dihydro-1H-imidazol-2-yl)phenol)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1, was coupled to 4-aminobenzonitrile (Aldrich, 14,775-3), using the method of General Procedure 4. Treatment with ethylene diamine as described in Procedure 13 afforded the title compound.  
      MS+=487.0  
       1 H-NMR (DMSO-d6) δ 14.26 (br, 1H), 10.90 (br, 1H), 10.75 (br, 1H), 10.40 (br, 2H), 8.10 (m, 2H), 7.93 (m, 2H), 7.56 (m, 4H), 4.00 (s, 4H).  
     Example 40  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-fluoro-3-cyano-phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid was prepared as shown in General Procedure 1. The amine was purchased from Oakwood (Cat number 013105). The amine and the pyrazole acid were coupled as shown in General Procedure 4.  
      MS+=461.9  
       1 H-NMR (DMSO-d6) δ 14.20 (b, 1H), 10.86 (b, 1H), 10.66 (s, 1H), 8.32 (b, 1H), 8.14 (b, 1H), 7.58 (m, 5H).  
     Example 41  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1-methyl-pyrazole-3-carboxylic acid (4-[1,4′-bipiperidin]-1′-yl-phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid prepared by coupling methyl ester 73 (General Procedure 15) with 2-chlorobenzoyl chloride (21) followed by hydrolysis and bromination using the method of General Procedure e 1 was coupled with 4-[1,4′-bipiperidin]-1′-yl-phenylamine (WO 2/099388) using the method of General Procedure 4.  
      MS+=600.0  
       1 H-NMR (CD3OD) δ 6.69 (d, J=6.0 Hz, 1H), 7.59-7.49 (m, 5H), 7.02 (d, J=9.0 Hz, 2H), 3.98 (s, 3H), 3.77 (d, J=12.0 Hz, 2H), 2.75-2.67 (m, 6H), 2.53 (m, 1H), 2.06-2.03 (m, 2H), 1.78-1.67 (m, 6H), 1.53 (m, 2H)  
     Example 42  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1-methyl-pyrazole-3-carboxylic acid [4-(4-pyridin-4-yl-piperazin-1-yl)phenyl]amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid prepared by coupling methyl ester 73 (General Procedure 15) with 2-chlorobenzoyl chloride (21) followed by hydrolysis and bromination using the method of General Procedure 1 was coupled with 4-(4-pyridin-4-yl-piperazin-1-yl)phenylamine (WO 2/099388) using the method of General Procedure 4.  
      MS+=594.0  
       1 H-NMR (CD3OD) δ 8.15 (d, J=5.7 Hz, 2H), 7.65-7.49 (m, 3H), 7.42-7.38 (m, 3H), 7.03 (d, J=9.0 Hz, 2H), 6.90 (d, J=6.3 Hz, 2H), 3.89 (s, 3H), 3.61-3.55 (m, 4H), 3.33-3.25 (m, 4H)  
     Example 43  
     Preparation of 4-methyl-5-(2-fluorobenzoylamino)-1-t-butyl-pyrazole-3-carboxylic acid (4-(2-cyanoethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid prepared by coupling methyl ester 48 with 2-fluorobenzoyl chloride (Aldrich, 12,084-7) using the method of General Procedure 8 followed by hydrolysis using the method of General Procedure 9 was coupled with amine 66 using the method of General Procedure 4.  
      MS+=448.1  
       1 H-NMR (CDCl3) δ 8.76 (s, 1H), 8.14 (t, 1H, J=7.3 Hz), 8.01 (d, 1H, J=14.1 Hz), 7.64 (d, 2H, J=8.2 Hz), 7.59 (m, 1H), 7.34 (t, 1H, J=7.6 Hz), 7.20 (d, 2H, J=8.2 Hz), 2.93 (t, 2H, J=7.8 Hz), 2.61 (t, 2H, J=7.8 Hz), 2.21 (s, 3H), 1.67 (s, 9H).  
     Example 44  
     Preparation of 4-methyl-5-(2-fluorobenzoylamino)-1-(t-butyl)-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The compound from Example 43 was treated with ethylene diamine using the method of Procedure 13.  
      MS+=491.2  
       1 H-NMR (CDCl 3 ) δ 8.85 (s, 1H), 8.24 (d, 1H, J=13.1 Hz), 8.07 (t, 1H, J=7.7 Hz), 7.58 (m, 1H), 7.47 (d, 2H, J=8.2 Hz), 7.32-7.18 (m, 2H), 7.08 (d, 2H, J=8.2 Hz), 2.89 (m, 2H), 2.69 (m, 2H), 2.23 (br s, 4H), 2.12 (s, 3H), 1.64 (s, 9H).  
     Example 45  
     Preparation of 4-methyl-5-(2-fluorobenzoylamino)-1H-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 8 using 2-fluorobenzoyl chloride instead of compound 21, was coupled to 4-aminobenzyl cyanide (Aldrich, A4,205-0) using the method of General Procedure 4. Treatment with ethylene diamine using the method of General Procedure 13 afforded the title compound.  
      MS+=435.2  
     Example 46  
     Preparation of 4-methyl-5-(2-fluorobenzoylamino)-1-phenyl-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The compound from Example 47 was treated with ethylene diamine using the method of General Procedure 13.  
      MS+=511.2  
       1 H-NMR (CD-3OD) δ 7.43-7.71 (m, 5H), 7.23-7.32 (m, 3H), 3.85 (s, 3H), 3.31 (s, 2H), 2.78-3.01 (m, 4H), 2.32 (s, 2H).  
     Example 47  
     Preparation of 4-methyl-5-(2-fluorobenzoylamino)-1-phenyl-pyrazole-3-carboxylic acid (4-(2-cyanoethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared as described in General Procedure 1 using 5-amino-4-methyl-1-phenyl-1H-pyrazole-3-carboxylic acid ethyl ester (prepared as described in General Procedure 16 using 3-cyano-3-methyl-2-oxopropanoic acid ethyl ester (U.S. Pat. No. 4,652,669)) in place of compound 20 and 2-fluorobenzoyl chloride in place of compound 21, was coupled to 4-aminobenzyl cyanide (Aldrich, A4,205-0)) using the method of General Procedure 10.  
      MS+=490.1  
       1 H-NMR (CDCl3) δ 8.89 (s, 1H), 8.07-8.18 (m, 2H), 7.72 (d, 2H, J=9.3 Hz), 7.60-7.64 (m, 3H), 7.47-7.56 (m, 3H), 7.37 (t, 1H, J=7.5 Hz), 7.20-7.28 (m, 3H), 3.00 (t, 2H, J=7.5 Hz), 2.67 (t, 2H, J=7.5 Hz), 2.43 (s, 3H).  
     Example 48  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1-methyl-pyrazole-3-carboxylic acid (4-(3-aminopropyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared by coupling methyl ester 73 (General Procedure 15) with 2-benzoyl chloride (21) followed by hydrolysis and bromination as shown in General Procedure 1, was coupled to aniline 70 (General Procedure 14) using the method of General Procedure 4. Treatment with TFA afforded the title compound.  
      MS+=490.0  
       1 H-NMR (DMSO-d6) δ 7.60 (m, 6H), 7.16 (m, 2H), 3.87 (s, 3H), 2.76 (m, 2H), 2.59 (m, 2H), 1.79 (m, 2H).  
     Example 49  
     Preparation of 4-Bromo-5-(2-chlorobenzoylamino)-1-methyl-pyrazole-3-carboxylic acid (4-(2-(4,5-dihydro-1H-imidazol-2-yl)ethyl)phenyl)amide  
     
       
         
         
             
             
         
       
     
      The pyrazole acid, prepared by coupling methyl ester 73 (General Procedure 15) with 2-benzoyl chloride (21) followed by hydrolysis and bromination as shown in General Procedure 1, was coupled to 4-aminobenzyl cyanide (Aldrich, A4,205-0) using the method of General Procedure 4. Treatment with ethylene diamine using the method of General Procedure 13 afforded the title compound.  
      MS+=529.1  
       1 H-NMR (DMSO-d6) δ 7.73 (m, 3H), 7.52 (m, 3H), 7.24 (m, 2H), 3.91 (s, 3H), 3.80 (s, 4H), 2.96 (m, 2H), 2.83 (m, 2H).  
     Biological Example  
      The potency and efficacy to inhibit the bradykinin B 1  receptor was determined for the compounds of this invention in a cell-based fluorescent calcium-mobilization assay. The assay measures the ability of test compounds to inhibit bradykinin B 1  receptor agonist-induced increase of intracellular free Ca +2  in a native human bradykinin B 1  receptor-expressing cell line.  
      In this example, the following additional abbreviations have the meanings set forth below. Abbreviations heretofore defined are as defined previously. Undefined abbreviations have the art recognized meanings. 
          BSA=bovine serum albumin     DMSO=dimethylsulfoxide     FBS=fetal bovine serum     MEM=minimum essential medium     mM=millimolar     ng=nanogram     μg=micrograms     μM=micromolar        

      Specifically, calcium indicator-loaded cells are pre-incubated in the absence or presence of different concentrations of test compounds followed by stimulation with selective bradykinin B 1  receptor agonist peptide while Ca-dependent fluorescence is monitored.  
      IMR-90 human lung fibroblast cells (CCL 186, American Type Tissue Collection) are grown in MEM supplemented with 10% FBS as recommended by ATCC. Confluent cells are harvested by trypsinization and seeded into black wall/clear bottom 96-well plates (Costar #3904) at approximately 13,000 cells/well. The following day, cells are treated with 0.35 ng/mL interleukin-1β in 10% FBS/MEM for 2 hours to up-regulate bradykinin B 1  receptors. Induced cells are loaded with fluorescent calcium indicator by incubation with 2.3 μM Fluo-4/AM (Molecular Probes) at 371C for 1.5 hrs in the presence of an anion transport inhibitor (2.5 mM probenecid in 1% FBS/MEM). Extracellular dye is removed by washing with assay buffer (2.5 mM probenecid, 0.1% BSA, 20 mM HEPES in Hank&#39;s Balanced Salt Solution without bicarbonate or phenol red, pH 7.5) and cell plates are kept in dark until used. Test compounds are assayed at 7 concentrations in triplicate wells. Serial dilutions are made in half log-steps at 100-times final concentration in DMSO and then diluted in assay buffer. Compound addition plates contain 2.5-times final concentrations of test compounds or controls in 2.5% DMSO/assay buffer. Agonist plates contain 5-times the final concentration of 2.5 nM (3×EC50) bradykinin B 1  receptor agonist peptide des-Arg 10 -kallidin (DAKD, Bachem) in assay buffer. Addition of test compounds to cell plate, incubation for 5 min at 351C, followed by the addition of bradykinin B 1  receptor agonist DAKD is carried out in the Fluorometric Imaging Plate Reader (FLIPR, Molecular Devices) while continuously monitoring Ca-dependent fluorescence. Peak height of DAKD-induced fluorescence is plotted as function of concentration of test compounds. IC 50  values are calculated by fitting a 4-parameter logistic function to the concentration-response data using non-linear regression (Xlfit, IDBS (ID Business Solutions Ltd.)).  
      Typical potencies observed for bradykinin B 1  receptor agonist peptides are EC 50  approximately 0.8 nM and approximately 100 nM for des-Arg 10 -kallidin and des-Arg 9 -bradykinin, respectively, while for bradykinin B 1  receptor antagonist peptide des-Arg 10 , Leu 9 -kallidin IC 50  is approximately 1 nM.  
      The compounds of this invention have potency in the above assay as demonstrated by results of less than 50 micromolar. It is advantageous that the assay results be less than 1 micromolar, even more advantageous for the results to be less than 0.5 micromolar.  
      In view of the above, all of these compounds exhibit bradykinin B 1  receptor antagonistic properties and, accordingly, are useful in treating disease conditions mediated at least in part by bradykinin B 1  receptor.