Patent Publication Number: US-2015087009-A1

Title: Live cell viability modification system and method

Description:
RELATED APPLICATION 
     The application is a continuation-in-part of and claims the benefit of the filing date pursuant to 35 U.S.C. §120 of U.S. patent application Ser. No. 13/868,330, for an ELECTRO MEDICAL TOOL SYSTEM, filed Apr. 23, 2013. 
    
    
     FIELD OF THE INVENTION 
     The present invention may relate to methods and apparatus intended to accelerate the healing rates of hard and soft human and/or animal tissues and promote regeneration of damaged organs using electrical and electromagnetic stimulation. This present invention may also relate to devices, systems, and methods that may sense electrical and/or molecular characteristics of at least one live cell, and more particularly, this invention may relate to devices, systems, and methods that may induce selective electrical changes in at least one live cell, wherein said selected electrical changes may catalyze molecular and/or chemical changes within the at least one live cell. 
     BACKGROUND OF THE INVENTION 
     In every arena of medical practice, the healing of tissues is the primary problem that must be dealt with. Trauma induced contusions; abrasions, organ failures, and bone damage are medical issues dealt with by the millions daily. Typical medical approaches to trauma include stitches, bandages, casts, as well as simple and complex mechanical restructuring, then letting the body takes its natural healing course. Disease is rampant in current society as evidenced by the flood of “pills” offered on television and pharmacy shelves. The bulk of these products rarely heal the tissues themselves, but seek to neutralize the symptoms resulting in side effects that are often worse than the disease itself. Non-invasive tissue regeneration tools that have no side effects are needed. 
     Key prior art patents that may relate to the present invention may be presented herein with summaries of their abstracts. 
     A Yoshida et al. U.S. Pat. No. 5,922,209 may describe a process for deactivating or destroying microorganisms by applying electrical energy to a microorganism through a liquid, gas or solid having electrical energy to cause an increase in an electric charge in excess of the limit of intracellular and extracellular electrostatic capacity possessed by the microorganism, which in turn results in an irreversible change in the microorganism cells and/or explosively destroys the border membrane of the microorganism cells. 
     Chang&#39;s U.S. Pat. No. 5,304,486 may disclose a method of and apparatus for cell portion and cell fusion using radiofrequency electrical pulses. The method may be used to fuse or porate a variety of cells comprising animal cells, human cells, plant cells, protoplasts, erythrocyte ghosts, liposomes, vesicles, bacteria and yeasts. The method may also be used to produce new biological species, to make hybridoma cells which produce animal or human monoclonal antibodies and to insert therapeutic genes into human cells which can be transplanted back into the human body to cure genetic diseases. 
     Saban, et al. U.S. Pat. No. 6,790,341 may provide microband electrode array sensors for detecting the presence and measuring the concentration of analytes in a sample. The microband electrodes of the invention have both a width and thickness of microscopic dimensions. Preferably the width and thickness of the microband electrodes are less than the diffusion length of the analyte(s) of interest. The electrodes are separated by a gap insulating material that is large enough that the diffusion layers of the electrodes do not overlap such that there is no interference and the currents at the electrodes are additive. 
     Edwards, et al. U.S. Pat. No. 5,472,441 may disclose a device for treating body tissues containing cancerous cells or non-malignant tumors with RF ablation, alone or in combination with systemic or localized chemotherapy. 
     Harris, et al. U.S. Pat. No. 6,400,487 may teach methods and apparatus for screening large numbers of chemical compounds and performing a wide variety of fluorescent assays, comprising live cell assays. The methods utilize a laser line scan confocal microscope with high speed, high resolution and multi-wavelength capabilities and real time data-processing. 
     Chang&#39;s U.S. Pat. No. 8,278,629 may disclose live-cell observation equipment for a non light-transmitting microscope to study temperature-dependent events and method thereof. 
     Hofmann&#39;s U.S. Pat. No. 4,561,961 may describe a cooled microscope slide and electrode apparatus for use in live cell fusion system employing tubular electrodes so fluid may be pumped through the electrodes to dissipate heat to enhance the yield of viable hybrids. An alternate embodiment sandwiches a gasket and parallel tubular electrodes between glass slides to permit cell fusion in a closed sterile environment. 
     This inventor&#39;s own Letovsky U.S. Pat. No. 6,825,792 may disclose a frequency based missile detection and neutralization system that uses some similar components and creates some similar effects in non-organic compounds as the present invention does in organic compounds. This inventor&#39;s published patent application Letovsky 20110001064—as well as the parent patent from which it is a divisional—discloses aspects related to the present invention without comprising the camera image data to waveform generator feedback loop, the variable color source, direct contact electrical to cell system, and non contact electromagnetic frequency application components specification provided herein which are necessary to make the present invention function as intended. 
     In addition, the bioelectromagnetics and the therapeutic electro-medical tool industries have been evolving for over a hundred and fifty years in many parts of the world. However, the western medical mainstream is just starting to embrace these industries as providing effective alternatives to drug therapies. Electrical energy used in medical applications is applied to the body by physical contact with conducting electrodes or is radiatively coupled through a transducer that may be a coil, a waveguide, a light source, or other electromagnetic energy emitter. Most of the available tools use electrical signals drawn from effects observed over decades of trial and error. 
     There may be prior art related to sensing the electrical characteristics of live cells. The primary tools in this area are patch clamps, electrical impedance spectroscopy systems, and multi-electrode array probes and head stages. Patch clamps look for specific current information in response to electrical and chemical stimuli, electrical impedance spectroscopy looks at cellular impedance changes in response to said stimuli, and multi-electrode arrays are intended to provide data on neural responses to direct current electrical stimuli. 
     There may be also prior art related to sensing the molecular characteristics of live cells. The primary tools in this area are spectroscopy systems that are wavelength specific such as Raman, infrared absorption, and x-ray spectrometers. 
     Radiation from the sun, space, and the atmosphere, as well as brainwaves, bioelectric signals, water, and food chemistry drive living cell metabolic processes. When an organism&#39;s metabolism is out of balance with its baseline genetic programming, disease is the result. The electro medical tool industry uses electrical and electromagnetic frequencies from several hertz to light waves to assist in this metabolic rebalancing. The industry is now into its second century of evolution having begun largely in Eastern Europe. 
     Russian documentation detailing electrical physiotherapies goes from present day back to the mid 1800s, and incorporates sound, ultrasound, radio frequencies and specific light frequencies. Japanese carbon arc light healing tools date back to the second world war—healing radiation burn victims after atomic bombs were dropped on Hiroshima and Nagasaki. These highly specific carbon compounds create intense light outputs designed to reproduce specific combinations of light wave frequencies to expedite the body&#39;s natural healing processes. Clinical documentation of the effectiveness of this technology is very broad. 
     Scientists at the University of Alberta in Canada have successfully regenerated teeth from the root up—by the application of specifically configured 1.5 megahertz pulses. Electrical bone growth stimulation is now common throughout the world using specifically configured frequencies at 76.4 hertz, 40 kilohertz, and 1.5 megahertz to increase the speed of bone growth after a fracture or surgery. 
     Therapeutic “cold” lasers have been proven to increase cell metabolism, increase collagen synthesis for increased healing of soft tissues, increase osteoblast production for increased healing of bone, increase circulation through increased formation of new capillaries by release of growth factors, increase T-cell production for increased immune function, increase production of neurotransmitters such as endorphins, serotonin, ACTH, etc., and increase chronic pain threshold through decreased C-fiber activity. 
     Cancer cure rates with chemotherapy and radiation—though increasing significantly in certain types of cancers—are still lacking in long term cancer cell elimination after decades of research and billions of dollars spent. Electro therapy tools in global research centers are starting to gain traction as being potentially useful for targeting cancer cells while leaving the immune system and neighboring healthy cells undamaged. 
     In the U.S. (United States), electro medical therapy home use products are now showing up everywhere—mostly “copy catting” each other with a very small number of frequencies used in therapeutic ultrasound, therapeutic lasers, TENS machines, galvanic skin stimulators, frequency specific microcurrent generators, etc. Often, beneficial results of these tools are “hit or miss” with users—yet scores of these product offerings in the market. In general the electro therapy tools available to the public are limited in their accuracy and effectiveness due to a lack of direct testing on live cells both inside and outside living bodies due to a lack of combined observation, analysis, and affectation tools. Some embodiments of the present invention are designed to fill this need. 
     Further, all living cells, e.g. eukaryotic cells, may be electrically active. Heart cells grown outside a body in-vitro will all synchronize and beat together even though they are not part of a complete heart. Cells operate and communicate with other cells both electrically and chemically. Live cells can be affected by electric stimulation as in heart pacemakers. Cells also emit electrical frequencies and voltages that can be measured with tools like electroencephalographs and electrocardiographs. Neural tissues generate oscillatory activity in many ways, driven either by mechanisms localized within individual neurons or by interactions between neurons. An electric eel can discharge electrical bursts up to 600 volts at lethal currents. 
     Many diseases may be essentially a condition wherein operational processes of at least one cell may behave in an “aberrant” manner relative to the behavior of healthy cells of the same type. This behavior can take the form of shape variations, genetic variations, electrical charge and waveform expression variations, molecular variations, and/or chemical variations. 
     The pharmaceutical industry produces specific drugs to attempt to direct a disease condition back towards a “normal” condition, i.e. healthy state. Almost universally, there are undesired side effects in this approach. A primary objective of the present invention is to provide a device, system, and/or method for selectively redirecting the viable processes of a diseased live cell and/or a diseased live cell group back towards a “normal” condition. Another objective of the present invention is to cause cells that may be eluding the attention of the immune system to change their electrical “identification” processes so they may be recognized by the immune system. 
     The following reference list may represent relatively recent prior art disclosures that may be relevant to some embodiments of the present invention: 
     U.S. Pat. No. 7,993,906—Closed-loop electrical stimulation system for cell cultures
 
U.S. Pat. No. 8,728,139—System and method for energy delivery to a tissue using an electrode array
 
U.S. Pat. No. 8,718,756—Optimizing characteristics of an electric field to increase the field&#39;s effect on proliferating cells
 
U.S. Pat. No. 8,706,261—Treating a tumor or the like with electric fields at different frequencies
 
U.S. Pat. No. 8,447,396—Treating bacteria with electric fields
 
U.S. Pat. No. 8,406,870—Treating cancer using electromagnetic fields in combination with other treatment regimens
 
U.S. Pat. No. 7,890,183—Treating parasites with electric fields
 
U.S. Pat. No. 7,722,606—Device and method for destruction of cancer cells
 
U.S. Pat. No. 7,519,420—Apparatus for selectively destroying dividing cells
 
     The present invention is the result of many years of confidential research into electrical and electromagnetic stimulation of cells to promote accelerated healing, the potential reactivation of stem cell activity, hard and soft tissue regeneration, as well as immune system stimulation to combat cancer, heart disease, and general autoimmune dysfunction. 
     No prior art live cell electrical signal and molecular signature sensing and affecting system exists that is configured to capture detailed electrical signal data from live cells, reduce that data to subsets and electrical “words”, create a “dictionary” of live cell electrical word definitions, and then transform live cell processes using electrical words drawn from said electrical signal dictionary. The present invention provides this capability. 
     SUMMARY OF THE INVENTION 
     The present invention may integrate wideband frequency generators, transducers, sensors, frequency analyzers, wavelength meters, light power meters, cameras, computers, and computer software to discover “bioactive frequencies” through computer image analysis of the effects of electrical and electromagnetic frequencies on living tissues to promote accelerated healing. 
     The present invention may also incorporate a database with fields and lookup tables populated from tissue and cell reactions data derived from real time image capture and event tracking software that locks on to cell behavior changes in response to electrical and electromagnetic frequencies applied to the cells. These datasets are continually updated as effects are observed and quantified, and the electrical and electromagnetic frequencies are automatically modified and augmented to accelerate beneficial changes. For example, if a certain frequency clearly speeds up cell division during a frequency sweep, that frequency may be locked in, and harmonics of that frequency at different points in the electromagnetic spectrum may be added with the goal of further accelerating beneficial changes in cell behavior while reducing the electrical power required. 
     In the present invention, frequencies ranging from DC to x-rays—comprising sub nanometer resolution monchromated light—are applied to live cell samples for both observation and affectation. Both non contact electromagnetic field generating transducers in close proximity to a live cell sample, and direct electrical application to live cell samples through electrodes fitted to microscope slides are used to apply the frequencies to the cells. The electrode fitted microscope slides may incorporate multiple electrical contact zones to apply voltage and current at various frequencies to live cell samples, read the changes in the impedance of the samples, and read the characteristics of the frequency waves (sine, square, etc.) passing through the samples. There may be a minimum of three contact points per zone—positive in, positive out, and ground—with all zones able to be wired in parallel or dealt with separately. The voltage levels may be quite high—up to 400 volts—but the current may be small—microamp to milliamp levels. Each zone may be 20 by 20 microns or smaller. This may be achieved with clear conductive overlays like cell phone touch screen flexible conductive films affixed to microscope slides. 
     Impedance matching may be necessary to correctly apply the required frequency and energy level outputs to induce desired results since tissue density and impedance changes with body depth and any electrical or electromagnetic frequency applied. Tissue simulators are used in ultrasound transducer calibration and may also be used in the present invention to set benchmarks for signal amplitude output ratios and impedance matching to account for the differences in signal penetration between a fully functional living body and a sample containing just a few living cells. 
     In some embodiments of the present invention, a high gain amplifier may be coupled to a live cell direct contact electrical signal-sensing array. Said array may be in turn coupled to an amplifier and an analog to digital converter and/or a computer to detect, record, and digitize the natural electrical signals, pulses, and waveforms that a given cell type may use to communicate with its neighbors, the immune system, and the organism in which it is operating, as well as the electrical signals, pulses, and waveforms that may define its particular natural operational electrical signature. 
     Specific parts of the recorded electrical signals that are determined to be relevant to specific cellular functional processes may be extracted from the recordings as discreet waveforms—or “words”—in waveform editing software, and converted into a file format that may then be loaded into an arbitrary waveform generator. The resulting cellular signal words may be played back through an amplifier that is coupled to either an indirect radiating electrical transducer or coupled to direct electrical contacts applied to the cells in vitro, the organism, the body, or the cells within the body to affect the cells&#39; electrical behavior. 
     In the present invention, electrical signals may be captured and analyzed from both healthy and diseased cells from the same or different cell types both in-vitro and in-vivo. Subsets (words) of the recordings may be extracted and “played back” to the cells whose behavior is to be modified. For example, the behavior change “catalyzing signal” may be extracted from a diseased cell or a cancer cell exhibiting a “death” signal in response to a chemical or electrical stimulus. The application of this signal may induce a death effect in the remaining cancerous or otherwise diseased cells. It is well known that all cells have a “death” trigger signal (apoptosis) inherent in their operational mechanics that kicks in when the body or that particular tissue is no longer capable of continuing to operate in a viable state. However, the exact electrical structure of these signals is not known in prior art. The present invention may reveal these signals and the structure of these signals. 
     In another example of the use of the present invention, an electrical signal derived from a healthy cell may be applied to a diseased cell to trigger it to return to a healthy state. 
     In another example of the use of the present invention, a signal from a healthy cell that indicates its type—such as liver signal—may be applied to a cancer tumor made up of liver cells that have metastasized to a lung and are “hiding” by putting out signals that direct the immune system to ignore it. The application of certain electrical “words” may induce the tumor cell group to announce its existence to the immune system—thereby activating the body&#39;s own defenses against the tumor—causing remission of the cancer condition. 
     A primary objective of the present invention may be to expand a catalog of frequencies used in the electro medical tool industry by providing a research toolset to observe and affect live cells and live tissue samples in real time with high resolution, high contrast live cell imaging analysis under both direct electrical contact and non-contact electromagnetic wave stimulation. The present invention may provide better frequency choices to enhance the healing processes in virtually every cell type in human and other animal bodies. 
     An objective of the present invention may be to define electrical and electromagnetic frequencies that may promote the enhanced uptake of beneficial drugs, vitamin and mineral compounds in a living organism. 
     Another primary objective of the present invention may be to identify and record specific electrical signals over time that may be inherent in healthy and/or diseased living cells, extract certain electrical signal subsets of said electrical signals, and then reapply those electrical signal subsets—or modified versions of those electrical signal subsets—to cells in a precise manner to selectively change specific functional electrical characteristics of living cell or tissue. The device, system, and/or method may be used to direct diseased or aberrant cells operating in the body to return to a normal state—or in the case of cancer cells for example—to cause the cancer cells to be recognized by the immune system and subsequently destroyed by the immune system. 
     Another objective of the present invention is to create a “dictionary” of live cell communications, with definitions associated with the “words” extracted from the electrical vocabulary used by all cell types—both healthy and diseased—in a human or other animal body. 
     The above, and other objects, features and advantages of the present apparatus will become apparent from the following description read in conjunction with the accompanying drawings, in which like reference numerals designate the same elements. A more complete understanding of the present invention, as well as further features and advantages, will be obtained by reference to the following detailed description and drawings. Preferred embodiments of the present invention will be described in the claims. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       Elements in the figures have not necessarily been drawn to scale in order to enhance their clarity and improve understanding of these various elements and embodiments of the invention. Furthermore, elements that are known to be common and well understood to those in the industry may not be depicted in order to provide a clear view of the various embodiments of the invention 
         FIG. 1  is a schematic key component diagram illustrating an electro medical tool optimization system per the present invention. 
         FIG. 2  is a slide per the present invention fitted with conductive traces contacting a cell sample. 
         FIG. 3  is a microscope head per the present invention fitted with transmission and pickup coils, as well as a conductive trace fitted slide. 
         FIG. 3A  is a microscope head per the present invention fitted with a waveguide, as well as a conductive trace fitted slide. 
         FIG. 4  is a cell sample having been affected by an electromagnetic frequency per the present invention. 
         FIG. 5  is a detail of a conductive trace pattern that may be applied to microscope slide per the present invention. 
         FIG. 6  is a series of simulated accelerated cell changes with cell perimeter and detail mapping per the present invention. 
         FIG. 6A  is a series of simulated accelerated cell changes with cell perimeter and detail mapping as well as cell uptake of a chemical compound per the present invention. 
         FIG. 7  is a schematic diagram of the process of the preferred embodiment of the present invention. 
         FIG. 8  is a schematic diagram of the process of the preferred embodiment of the present invention comprising molecular analysis functionality. 
         FIG. 9  is a chart recording of electrical signal outputs from a cell culture with PC3 confluent cancer cells. 
         FIG. 10  is a chart recording of electrical signal outputs from PC3 confluent cancer cells stressed with the introduction of an alcohol solution in highly diluted but toxic concentrations, indicating a waveform subset to be loaded into an arbitrary waveform generator. 
         FIG. 11  is a chart recording of electrical signal outputs from a PC3 confluent cancer cell culture that was treated with the waveform shown in  FIG. 10  for three minutes resulting in significantly reduced electrical amplitude output and observable electrical waveform output changes. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     A preferred embodiment of an electro medical tool optimization system in accordance with the present invention per the system flow chart schematic as shown in  FIG. 1  may provide the capability to detect a specific live cell  15  anywhere in a live cell sample  34  containing multiple cells, and subsequently apply electrical or electromagnetic signals to a cell sample  34  and modify the behavior of any specific cell  15 . Said electrical or electromagnetic signals may be drawn from a frequency range comprising DC through x-rays. The present invention is designed to effect live cells, observe and collect data on said effects, and use said data to optimize said effects to catalyze a specific cell function modification or neutralization. 
     The preferred embodiment of an electro medical tool optimization system in accordance with the present invention is shown in  FIG. 1  through  FIG. 6   a , and all said Figures with duplicate, expanded, or detailed renderings of specific elements will share the same numbers for said elements. In  FIG. 1  specifically, cell sample  34  and cell  15  are represented simultaneously below lens  18  and as part of a screen image in video display  60 . 
     As presented in  FIG. 1 , the present invention may include a microscope  10 , which may be configured with a monochromator  12  capable of splitting a white light source  14  into single nanometer or sub nanometer wavelengths  16  that may range from 200 to 1100 nanometers, but said wavelengths  16  in practice may only be limited by the state of the art in monochromator  12  technology. Monochromator  12  may output any of said wavelengths  16  through any cell  15  in a cell sample  34  and on through a lens  18  of microscope  10 . 
     Monochromator  12  may be operated manually or it may be electrically coupled to computer  62  that is configured with software program  63 . Software program  63  may be configured to direct monochromator  12  to provide desired specific wavelengths  16 . 
     Alternatively, software program  63  may be configured to control a variable color light source  24  that is electrically coupled to a computer  62  and capable of outputting any one of millions or billions of colors  17 , said colors  17  in practice may only be limited by the state of the art in computer software and variable color light source  24  technology. Source  24  may also be configured to output said colors  17  through any cell  15  in a cell sample  34  and on through a lens  18  of microscope  10 . Source  24  may be a video projector, an RGB color laser array, a super continuum laser, or any other variable color emitting light source that derives its color output commands manually by a user or from a software program  63  on a computer  62 . A dual beam combiner  11  may also be used to combine and aim the light outputs from monochromator  12  and source  24  through any cell  15 . 
     A waveform generator  26  with at least two channels of signal outputs  28  and  30 , may be electrically coupled to a wideband power amplifier  27  input  29  through waveform generator output  28 . Amplifier  27  output  31  may be electrically coupled to an electromagnetic field output transducer  32  which may be mechanically mounted to microscope  10  to surround a microscope lens  18  to induce an electromagnetic field into any cell  15  in cell sample  34  placed on a microscope stage  35 . In certain cases, waveform generator  26  may be also be electrically coupled directly to electromagnetic field output transducer  32  through waveform generator output  28  by bypassing amplifier  27 . Electromagnetic field output transducer  32  may be single transducer or a plurality of transducers configured to generate an electromagnetic field in response to any waveform  9  provided by waveform generator  26 . In certain frequency ranges electromagnetic field output transducer  32  may be a waveguide as shown in  FIG. 3A . 
     Waveform generator  26  may output any standard function generator waveform  9  comprising but not limited to sine, square, ramp, pulse, noise, DC, as well as a user defined waveform  9  with sweep functionality, variable duty cycle, variable amplitude, and variable frequency range from DC up through the AC electromagnetic spectrum. The Anritsu MG3690C with frequency extender options can cover near DC to 500 gigahertz in one box. For the purposes of the present invention said waveform generator  26  frequency range in practice may only be limited by the current state of the art. 
     Waveform Generator  26  is also connected to computer  62  through instrument interface  76  to allow data transfer and functional control by software program  63 . All components presented herein are connected to computer  62  through instrument interface  76  for bidirectional data transfer. These data couplings between computer  62  and all other components described in the present invention may be USB, RS232, Firewire, GPIB, or any other industry standard instrumentation data interface. The electrical signal couplings between all components may be BNC cables or any other industry standard. 
     Power amplifier  27  should have frequency response equal to waveform generator  26 . waveform generator  26  may also have an input  25  which is electrically coupled to computer  62 . Electromagnetic field output transducer  32  should have frequency response equal to waveform generator  26 . 
     The input  37  of a wideband amplifier  40  may be electrically coupled to an electromagnetic pickup transducer  42  which may be mechanically mounted concentrically to transducer  32  on microscope  10  such that it can detect any electromagnetic waves that may be applied to the proximity of any cell  15  in cell sample  34  by electromagnetic transducer  32 . Amplifier  40  ideally may eliminate any impedance mismatches in pickup transducer  42  as any waveform  9  may be applied to transducer  32  from waveform generator  26  through power amplifier  27 . Amplifier  40  may have the same frequency response as amplifier  27 . 
     Electromagnetic pickup transducer  42  may be a single transducer or a plurality of transducers configured to sense an electromagnetic field in response to any waveform  9  provided by waveform generator  26 . In certain frequency ranges electromagnetic pickup transducer  42  may be a waveguide as shown in  FIG. 3A . 
     An oscilloscope  36  input channel  33  and a spectrum analyzer  38  input channel  65  may be electrically coupled in parallel to the output  39  of amplifier  40 . Oscilloscope  36  and spectrum analyzer  38  may have the same frequency response as waveform generator  26  and may be used to insure that any waveform  9  applied to transducer  32  are in fact reaching cell sample  34 . Oscilloscope  36  and spectrum analyzer  38  may also be directly electrically connected to transducer  42  and in certain configurations of the present invention by bypassing amplifier  40 . Additionally, oscilloscope  36  and spectrum analyzer  38  functions may be incorporated into a single spectrophotometer device. 
     In the preferred embodiment of the present invention in  FIG. 1 , waveform generator  26 , wideband power amplifier  27 , microscope stage  35 , wideband amplifier  40 , oscilloscope  36 , spectrum analyzer  38 , wideband power amplifier  48 , wideband amplifier  52 , camera  58 , wavelength meter  70 , and optical power meter  72  may all be electrically coupled to computer  62  through industry standard instrumentation interface  76  to allow functional control by software program  63 . 
     As detailed in  FIG. 2 , cell sample  34  may also be contained on an electrically conductive microscope slide  44 . Slide  44  may be configured with a minimum of three electrically conductive traces  45 ,  46 , and  47  configured to make electrical contact with any cell  15  in cell sample  34 . 
     Referring again to  FIG. 1 , slide  44  and traces  45 ,  46 , and  47  are shown expanded on display  60  to further clarify their configuration. Trace  46  may be the ground connection for input trace  45  and output trace  47 . Slide  44  input trace  45  may be electrically connected to wideband power amplifier  48  output  49 . Input  50  on amplifier  48  may be electrically connected to output  30  of waveform generator  26 . Power amplifier  48  should have frequency response equal to waveform generator  26 . 
     Slide  44  output  47  may be electrically connected to input  51  of a wideband amplifier  52 . Amplifier  52  output  53  may be electrically coupled in parallel to oscilloscope  36  second input channel  55  and spectrum analyzer  38  second input channel  56 . Amplifier  52  ideally may eliminate impedance mismatches in any cell  15  in a cell sample  34  as any waveform  9  may be applied to traces  45  and  47  from waveform generator  26  through power amplifier  48 . Amplifier  52  should have the same frequency response as waveform generator  26 . 
     Camera  58  may be mounted on a beam splitter  81  on said microscope  10  so as to view cell sample  34  through lens  18 . A video display  60  input  61  may be coupled to video output  59  of camera  58  to allow a user to monitor any effect of waveform generator  26  waveform  9  on any cell  15  in a cell sample  34 . Additionally, video output  59  may be also electrically coupled to computer  62  through instrument interface  76 . Software program  63  may be configured to track and map any cell  15  in cell sample  34  and populate database  64  with cell  15  size and cell mechanics data derived from image data provided by camera  58 . 
     Baseline cell behavior information  68  may be included in database  64 . Baseline cell behavior information  68  may include typical cell size for a given cell type, rate of mitosis, molecular pathway openings and closings relative to certain chemical compounds, etc. Software program  63  may be configured to detect any cellular behavior alteration  69  from camera  58 . Cellular behavior alteration  69  may include any cell  15  behavior deviation from a baseline cell behavior information  68 , comprising such changes as size and shape. Baseline cell behavior information  68  may be refreshed with respect to initial cell  15  size data at the start of every experiment. 
     Wavelength meter  70  and optical power meter  72  may also be installed on beam splitter  81  on microscope  10  to log the spectral information of a cell sample  34  before and after application of said waveforms  9  from waveform generator  26 . Wavelength meter  70  and optical power meter  72  may be electrically coupled to computer  62  through instrument interface  76  and information they provide may continually populate relevant fields in database  64 . Software program  63  may be configured to modify the waveforms  9  in response to any cellular behavior alteration  69  occurring in response to any waveform  9 , light wavelength  16 , or light color  17 . Wavelength meter  70  and optical power meter  72  may have the same frequency response as monochromator  12  and source  24 . Wavelength meter  70  and optical power meter  72  may also be a single spectrometer device incorporating the functions of both. 
     As camera  58  image data updates database  64 , cellular behavior alteration  69  data may enable software program  63  to track hundreds of cells in a cell sample  34  simultaneously. 
     In database  64 , each cell  15  location within cell sample  34  on slide  44  may be represented in the x/y/z axes relative to a “zero” point on a three dimensional environment model mapped to the observable area of a microscope slide  44  in database  64  at a resolution of 0.2 microns, or a resolution only limited by current state of the art in optical lens technology. This type of “object of interest” microscopic targeting and tracking software is now available from an array of software providers. 
     Each cell  15  initial size may be logged with the same resolution of 0.2 microns, or a resolution only limited by current state of the art in optical lens technology. 
     Any cellular behavior alteration  69  data may be logged and updated in real time continually updating field data in database  64  as managed by software program  63 . 
     An array of statistical outputs from cellular behavior alteration  69  data may include: 
     a. real time updated position information of any cell  15  in environmental model.
 
b. acceleration/deceleration of any cell  15  in real time and over time.
 
c. expansion/contraction of any cell  15  in real time and over time.
 
d. ambient fluid flow into/out of any cell  15  in real time and over time.
 
     A user of the present invention may initially set waveform generator  26  to sweep a waveform  9  from DC up to the frequency limits of waveform generator  26  at a particular sweep rate not to exceed the image and data acquisition limits of camera  58 . When any cellular behavior alteration  69  occurs, the waveform  9  being output at that moment may be locked in by a user or software program  63  and the signal amplitude may be raised or lowered or the pulse width or duty cycle may be altered. Waveform generator  26  may then be directed by a user or software program  63  to add a harmonic  13  of root waveform  9  to waveform  9 . Additional harmonics from  13   p  to  13   n , as well as wave shape and amplitude alterations may also be tested until no more cellular behavior alteration  69  occurs. 
     A user may also manually choose to lock-in or sweep through the light wavelength  16  outputs of monochromator  12  and variable color source  24  colors  17 . Software program  63  may also be configured to sweep through and lock in any wavelength  16  or colors  17  of light, or combinations of the light outputs of monochromator  12  and variable color source  24  at a particular sweep rate not to exceed the image and data acquisition limits of camera  58 . 
     Those frequency waves that are most absorbed by a cell  15  may be considered for the purposes of the present invention as indicated in  FIG. 6 , resonant frequencies  8  of a cell  15 . Said resonant frequencies  8  are a subset of any available waveform  9  and must initially be identified through laboratory experimentation, and are then integrated within database  64  as lookup tables. “Overdriving” the amplitude of said resonant frequencies  8  with respect to a base rate of frequency absorption of a cell  15 , said rate data contained in database  64 , may affect the electrical conductivity and the chemical and mechanical conditions of a cell  15 . These resonant frequencies  8  may then be manipulated and augmented by changes in pulse rate, amplitude, and wave shape, as well as the addition of frequency inversions, harmonics, and dissonances of said resonant frequencies  8  by software program  63  through computer  62  and waveform generator  26 . It may be the manipulation of these resonant frequencies  8 , combined with other waveforms  9  and one or more of harmonic  13  through  13   n  combinations that catalyze a cellular behavior alteration  69 . 
     Resonant frequencies  8 , combined with other waveforms  9  and harmonic  13  through  13   n  may be applied to catalyze destruction of a cancerous cell  15  mechanics—initially by altering a single specific structure or behavior within a cell  15 , and then outputting and altering additional applied waveforms  9  and harmonic  13  through  13   n  combinations, which may then propagate state changes in other cell  15  structures and mechanics like a domino effect—possibly allowing an immune system to recognize a cancer cell  15  within a cell sample  34  as an invader and dispatch white blood cells to destroy it. For example, if a molecule of a given component may be comprised of ten atomic elements arranged in a particular way, modifying the polarity of the third most abundant atomic element in the molecule may have such a catalyzing effect on a cell  15  mechanics. 
     Referring now to  FIG. 4  which is a photograph from a laboratory experiment using the present invention, sine wave frequencies of 727 hertz were applied to a cell sample  34  consisting of residue from a wine tank. Said cell sample  34  contained many bacteria and yeast cell  15  examples in large numbers. After a few minutes it was observed that almost a cell  15  elements migrated to the cover slip edges of slide  44 —leaving the middle of the cover slip area almost empty. This is a simple example of electromagnetic effects on organisms at the microscopic level. 
       FIG. 5  is one possible trace detail per the present invention that may be rendered on a clear conductive film that may be applied to a glass or polycarbonate microscope slide. Said clear conductive film traces  45 ,  46 , and  47  may be printed or separated by conductivity-neutralized areas. 
     Referring now to  FIG. 6 , a resonant frequency  8 , individually or combined with a waveform  9  and a harmonic  13  through  13   n  combinations may be applied to a cell  15  through traces  45  and  46  and/or electromagnetic transducer  32  to stimulate a cellular behavior alteration  69  in the form of simulated accelerated mitosis as seen in the series of cell  15  changes illustrated in the top row. In the bottom row of  FIG. 6 , said cellular behavior alteration  69  may be mapped (by software program  63  in response to image data derived from camera  58  as detailed in  FIG. 1 ) as black outline  80  around the perimeter of cell  15  and black outline  82  around the nucleus  83  of a cell  15 . The black arrows indicate electromagnetic waves radiating from electromagnetic transducer  32 . 
     Referring now to  FIG. 6A , a catalytic bioavailable media  78  which may include a drug, a vitamin, or a mineral compound, in conjunction with a resonant frequency  8 , individually or combined with a waveform  9  and a harmonic  13  through  13   n  combinations may be applied to a cell  15  through traces  45  and  46  and/or electromagnetic transducer  32  to stimulate a cellular behavior alteration  69  in the form of simulated accelerated mitosis as seen in the series of cell  15  changes illustrated in the top row. In the bottom row of  FIG. 6A , said cellular behavior alteration  69  may be mapped (by software program  63  in response to image data derived from camera  58  as detailed in  FIG. 1 ) as black outline  80  around the perimeter of cell  15  and black outline  82  around the nucleus  83  of a cell  15 . The black arrows indicate electromagnetic waves radiating from electromagnetic transducer  32 . 
     Many of the elements and software capabilities included in the present invention are available in various industries and disciplines so they are not detailed herein beyond the description presented. However, the present invention is a unique and novel system and apparatus combination that incorporates methods, features, and components not found in any other single apparatus or toolset. 
     There may be three primary aspects of all biological systems—mechanical, chemical, and electrical. The present invention may focus on the electrical signals, pulses, waveforms, and mechanisms that cells use to communicate to their neighbors, the brain, and the body. Cells&#39; electrical language may include both “sentences” and “words.” Electrically, cells may speak relatively slowly. Much of cellular electrical dialog may happen at similar speeds to low frequency brain waves, and some cellular electrical dialog may be much slower. Cellular electrical language may appear similar on many levels. While this inventor was preparing prototypes of the present invention, several neuroscientists were invited to review the raw waveform data extracted from various unstimulated live cell types. All of the neuroscientists pointed to parts of the waveform recordings as signals consistent with brain neuron activity, cardiac cellular activity, and/or muscular activity. All of these neuroscientists were shocked to learn that the signals were derived from small groups of live cells that were not neurons, cardiac cells, nor muscle cells. 
     The present invention may record the electrical “language” of healthy and diseased live cells. The details and differences—the “words”—of that language may be compared between healthy and diseased cells, and between varying cell types. Certain key words from the language may be extracted, reconfigured as to file type, and reapplied to re-educate badly behaving—or diseased—cells through repeated application of said words to modify the electrical condition of live cells toward a desired behavioral (viable) condition. 
     In some preferred embodiments of the present invention, a cell sample  34  may be grown on an electrode array  44 , or grown in a standard cell culture petri dish and may be electrically coupled to an overhead, drop down, variation of said electrode array  44 . The electrodes comprising said array  44  may be configured to provide multiple points of contact to create a pathway for electrical signals emanating from, and being applied to, a live cell  15  in a cell sample  34 . Array  44  may be configured as an interdigitated differential electrical contact with two positive leads as trace  45  and trace  47 , and a common ground  46 . Array  44  may also be used as a simple positive trace  45  and a negative connection  46  for single sided signal output and input. Pluralities of electrical traces in array  44  may provide greater live cell  15  or cell sample  34  surface area contact that may provide additional signal channel inputs or better signal to noise ratio. 
     In some preferred embodiments, array  44  as represented in  FIG. 7  and  FIG. 8  may be presented as an exemplary single interdigitated differential electrode configuration. Electrically conductive trace  45  and electrically conductive trace  47  in electrical signal array  44  may be used as differential positive signal inputs, and trace  46  is used as a ground connection. Array  44  may be electrically coupled to signal input amplifier  52  and signal output amplifier  48 . Array  44  may be substantially equivalent to electrical conductive slide  44 , or may be adhered to a different material substrate or even as discrete unmounted electrodes applied from any direction as long as contact is made with a live cell  15 , or a cell sample  34 , a live cell  105 , or a cell sample  104 . 
     In some preferred embodiments operational elements in the present invention may electrically interconnect through industry standard instrumentation interface  76 . Said interface  76  may not be indicated with a numeral in all of the drawing Figures herein, but is considered to be part of any preferred embodiment where it would be effective. 
     In a preferred embodiment as depicted in  FIG. 1 , cell  15  and/or cell sample  34  may provide electrical signal information to amplifier  52 , then said cell  15  and/or cell sample  34  may be replaced by cell  105  and/or cell sample  104 , wherein cell  105  and/or cell sample  104  may then be stimulated by the electrical signal output process of some embodiments of the present invention, wherein array  44  may be coupled to amplifier  48 . 
     Either trace  45  or  47  may be used as a signal positive in a non-differential configuration, but differential inputs may be inherently lower in noise than single ended inputs. Amplifier  52  may be a high variable gain, low noise amplifier such as a Grass JP511. However, any analog or analog to digital amplifier package that provides representative electrical signals characteristics of a cell  15  or cell sample  34  may be used. The Texas Instruments ADS family combines multi-channel analog preamplifiers with analog to digital conversion, and may function effectively. An analog amplifier may be used in some preferred embodiments because of subtleties that may be detectable in said cell  15  or cell sample  34 . For example, and without limiting the scope of the present invention, some such subtleties may include cellular reaction to electrical and/or chemical stimuli. 
     An AD Instruments Powerlab SP analog to digital converter  85  may be used in some preferred embodiments to convert the output from amplifier  52  to at least one plurality of electrical signals  92  derived from cell  15  or cell sample  34  so computer  62  may record said signals  92 . Said signals  92  may be representative of a baseline cell behavior information  68  being a first viable state of a cell sample  34  or a live cell  15 . 
     Any analog to digital converter  85  may be used that may be capable of correctly representing signals  92 . In some preferred embodiments of the present invention signals  92  derived from a group of cells in sample  34  may more accurately represent electrical intercellular communication in or out of a living body than deriving signals  92  from just one cell  15 . 
     At least one electrical signal “word” waveform  9  may be extracted from said signals  92  by at least one operator interface  110  electrically coupled to computer  62 , or by software program  63  operationally installed on computer  62 . Said at least one waveform  9  may be applied to at least one second live cell  105  or a second cell sample  104  to alter the state of viability from a cell baseline behavior  68  to a cell altered behavior  69 , or to an otherwise healthy or normal state. Said at least one first cell  15  and said cell sample  34 , and said at least one second cell  105  and said second cell sample  104 , may be similar or entirely different cell types. 
     In some embodiments, analog to digital converter  85  may capture signals  92  as amplitudes, voltages, and pulses over time as a digital file that may be recorded and processed by software program  63  to provide a subset of said signals  92  as at least one waveform  9 . Waveform  9  may then be uploaded to waveform generator  26  through software program  63 . Said software program  63  may incorporate file editing and conversion functions that may be substantially equivalent to those in a program such as “Easywave” available from Siglent, who may manufacture a version of arbitrary waveform generator  26 . Said software program  63  may also incorporate file conversion functions that may be substantially equivalent to those in the program “Chart” by AD Instruments, the maker of the Powerlab SP. Said Chart software may output waveform recordings as .wav files, .csv files, and the like. Other software such as Excel, Audigy, or Cool edit pro may also be used to convert .wav files into a waveform  9  may be uploaded into waveform generator  26 . The format for said waveform  9  may be a .csv file, an excel file, or any other file format that may be uploaded to an arbitrary waveform generator  26 . Any signals  92 , or said waveform  9  may be also be loaded into database  64  such that software program  63  may be configured to direct computer  62  to output an appropriate waveform  9  as depicted in  FIG. 10 , through amplifier  48  and array  44 , as depicted in  FIG. 7  and  FIG. 8 , to alter the state of viability from a cell baseline behavior  68  to a cell altered behavior  69  in said at least one second live cell  105  or said second cell sample  104 . Said cell altered behavior  69  may be represented by a second waveform signal  93  as depicted in  FIG. 11 . Said cell altered behavior  69  second waveform signal  93  may include altered cellular division, a change in immune system response, a change in molecular structure, entering a state of apoptosis, a change in metabolism, a change in DNA or RNA replication, a change in transcription, a change in translation, a change in cell signaling, a change in cell surface activity, a change in cell surface permeability, reactivation of stem cell functionality in adult differentiated cells, alteration of electrical conductivity or alteration of electrical conductivity in an ambient medium surrounding said at least one biological cell, and altered uptake of drugs, vitamin or mineral compounds by said at least one biological cell, or a state of apoptosis being triggered in said at least one second live cell  104  or said second live cell sample  104 . 
     Just as a pacemaker may alter the rhythms of a heart with subtle electrical signals, so may a waveform  9  may alter the electrical characteristics of a cell  105 , or a cell sample  104  toward a desired result. When a waveform  9  may be applied to a cell  105 , or a cell sample  104 , array  44  may be rewired to serve as an electrical signal output device wherein trace  45  and trace  47  may be used as parallel positive outputs and trace  46  may be used as a ground connection from amplifier  48 . Either trace  45  or trace  47  may used alone, but using the pair provides a larger surface area of electrical conductivity. Further, in certain embodiments of the present invention, amplifier  48  and amplifier  52  may be the same unit, simply switching input and output connections through interface  76  to array  44 . 
     In some preferred embodiments of the present invention, an electrical signal waveform  9  introduced into array  44  by waveform generator  26  through amplifier  48 , and applied to a cell  105 , or a cell sample  104 , may include direct current, direct current pulses or alternating current waveforms of any shape and frequency derived from a cell  15 , cell sample  34 , or the subsonic, acoustic, or electromagnetic spectrum. Array  44  electrode traces may be substantially equivalent to those on electrical conductive slide array  44  or may be adhered to a different material substrate or even as discrete unmounted electrodes. 
     As noted above, oscilloscope  36 , spectrum analyzer  38 , wavelength meter  70 , and/or optical power meter  72  may also be configured as a single spectrometer device incorporating the functions of either or all, to provide the function of detecting electromagnetic emission molecular signature data in a live cell  15 , cell sample  34 , live cell  105 , or cell sample  104 . In some embodiments, light source  24  may provide the required electromagnetic energy emission to excite molecular shifts, i.e. light source  24  may operate as an electromagnetic energy emission source. In some embodiments, camera  58  may provide the function of detecting electromagnetic emission molecular signature data in a live cell  15 , cell sample  34 , live cell  105 , and/or cell sample  104 , depending on the sensor such as an MCT, microbolometer, or thermopile chosen for said camera  58 , wherein said camera  58  may also function as molecular analyzer  101 . 
     Referring to  FIG. 8 , in some embodiments a molecular analyzer  101  may operate as an electromagnetic emission detector. In some embodiments, molecular analyzer  101  may comprise one or more of the functions of wavelength meter  70 , optical power meter  72 , camera  58 , oscilloscope  36 , spectrum analyzer  38 , and the like. Molecular analyzer  101  may be configured to directly view a live cell  15 , cell sample  34 , live cell  105 , and/or cell sample  104  in a manner that does not incorporate a microscope  10 . 
     Light source  24  as indicated above may provide any wavelength in the acoustic or electromagnetic spectrum, and as configured in some embodiments of the present invention, may be configured as an infrared energy emitter, a single wavelength laser, a quantum cascade laser, a supercontinuum laser, or any other source of electromagnetic waves able to excite molecular and atomic bonds so they may be sensed by said molecular analyzer  101 . Said molecular analyzer  101  may be a Raman spectrometer, an infrared absorbance spectrometer, or any other detector able to sense molecular data in response to electromagnetic waves emissions. 
     Some embodiments of the present invention may be intended to take advantage of improvement in the disclosed components as said improvements become available. Dispersive infrared, FT-IR infrared, X-Ray, and Raman adapters are available for microscopes, and as stand alone tools, so no further detail on these components is required. 
     Analyzer  101  may be electrically coupled to computer  62  such that the absorbed, reflected and transmitted details of any cell  15 , cell  105 , cell sample  34 , or cell sample  104 , as sensed by molecular analyzer  101  are defined by software program  63  as molecular signatures of any cell  15  or cell sample  34  and entered into appropriate field and columns in database  64 . 
     The benefit of incorporating real time molecular signature analysis may be that it provides an enhanced additional cell  15 , cell  105 , or cell sample  34  or cell sample  104  viability feedback mechanism to optimize a desired electrical effect on cell  105 , or cell sample  104 . 
     Using video display  60 , computer  62 , software program  63 , database  64 , and operator interface  110 , a user may manually derive a waveform  9  and load said waveform  9  into waveform generator  26  to create a desired change in electrical viability in a cell  15 , cell  105 , cell sample  34 , or cell sample  104 . The present invention may also operate automatically as disclosed herein and as detailed above. 
     Some embodiments, of the present invention as presented in  FIG. 7  may incorporate a configuration of array  44  as an EEG, EMG, or EKG (ECG) placement on a live animal or human subject to record said signals  92  from said live animal or human subject as brainwave or heartbeat signals  92  and apply said signals  92  or a waveform  9  subset of said signals  92  to a live cell  105  or a cell sample  104  to develop data relevant to the automatic and autonomic nervous system and cell behavior relationship to alter the state of viability from a cell baseline behavior  68  to a cell altered behavior  69 . 
     As presented in  FIG. 11 , a representative cell viability electrical waveform signal  93  indicates a dramatically lowered electrical signal output from a cell sample  105  after the application of a waveform  9  through array  44  after said waveform  9  was extracted from a signal  92  recording over time represented in  FIG. 10 , that resulted from the application of a toxic chemical stimulus to said first cell sample  34  signal  92  also shown in part in  FIG. 9  prior to said toxic chemical introduction, demonstrating that a preferred embodiment of the present invention may alter the state of viability from a cell baseline behavior  68  to a cell altered behavior  69 . In the upper left corner of  FIG. 9  (landscape orientation) a “sens” factor of thirty was used to acquire the unstimulated cell  15  baseline behavior  68 , and the same location on  FIG. 11  it can be seen that the “sens” setting was at five. That means it took approximately 6 times the gain setting to detect any electrical output from second cell sample  104  following application of waveform  9  extracted from said signals  92  depicted in  FIG. 10 . 
     While the invention has been particularly shown and described with reference to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made without departing from the spirit and scope of the invention.