Patent Publication Number: US-2022213149-A1

Title: Antigen delivery platforms

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This is a Divisional of U.S. patent application Ser. No. 16/114,621, filed Aug. 28, 2018, which is a Continuation of U.S. patent application Ser. No. 13/878,835, filed Oct. 10, 2013 (now abandoned), which is the U.S. National Stage application submitted under 35 U.S.C. § 371 for International Application No. PCT/US2011/55834, filed Oct. 11, 2011, which claims priority to U.S. Provisional Application No. 61/391,960, filed Oct. 11, 2010, all of which are incorporated herein by reference in their entireties. 
    
    
     SUBMISSION OF SEQUENCE LISTING ON ASCII TEXT FILE 
     The content of the following submission on ASCII text file is incorporated herein by reference in its entirety: a computer readable form (CRF) of the Sequence Listing (file name: VN54076D1_US_Seq_Listing.txt; created Jun. 28, 2021, size: 614,614 bytes). 
     BACKGROUND 
     Herpes viruses are widespread and cause a wide range of diseases in humans that in the worst cases can lead to substantial morbidity and mortality, primarily in immunocompromised individuals (e.g., transplant recipients and HIV-infected individuals). Humans are susceptible to infection by at least eight herpes viruses. Herpes simplex virus-1 (HSV-1, HHV-1), Herpes simplex virus-2 (HSV-2, HHV-2) and Varicella zoster virus (VZV, HHV-3) are alpha-subfamily viruses, cytomegalovirus (CMV, HHV-5) and Roseoloviruses (HHV-6 and HHV-7) are beta-subfamily viruses, Epstein-Barr virus (EBV, HHV-4) and Kaposi&#39;s sarcoma-associated herpesvirus (KSHV, HHV-8) are gamma-subfamily viruses that infect humans. 
     CMV infection leads to substantial morbidity and mortality in immunocompromised individuals (e.g., transplant recipients and HIV-infected individuals) and congenital infection can result in devastating defects in neurological development in neonates. CMV envelope glycoproteins gB, gH, gL, gM and gN represent attractive vaccine candidates as they are expressed on the viral surface and can elicit protective virus-neutralizing humoral immune responses. Some CMV vaccine strategies have targeted the major surface glycoprotein B (gB), which can induce a dominant antibody response. (Go and Pollard, JID 197:1631-1633 (2008)). CMV glycoprotein gB can induce a neutralizing antibody response, and a large fraction of the antibodies that neutralize infection of fibroblasts in sera from CMV-positive patients is directed against gB (Britt 1990). Similarly, it has been reported that gH and gM/gN are targets of the immune response to natural infection (Urban et al (1996) J. Gen. Virol. 77(Pt. 7):1537-47; Mach et al (2000) J. Virol. 74(24):11881-92). 
     Complexes of CMV proteins are also attractive vaccine candidates because they appear to be involved in important processes in the viral life cycle. For example, the gH/gL/gO complex seems to have important roles in both fibroblast and epithelial/endothelial cell entry. The prevailing model suggests that the gH/gL/gO complex mediates infection of fibroblasts. hCMV gO-null mutants produce small plaques on fibroblasts and very low titer virus indicating a role in entry (Dunn (2003), Proc. Natl. Acad. Sci. USA 100:14223-28; Hobom (2000) J. Virol. 74:7720-29). Recent studies suggest that gO is not incorporated into virions with gH/gL, but may act as a molecular chaperone, increasing gH/gL export from the ER to the Golgi apparatus and incorporation into virions (Ryckman (2009) J. Virol 82:60-70). Through pulse-chase experiments, it was shown that small amounts of gO remain bound to gH/gL for long periods of time but most gO dissociates and or is degraded from the gH/gL/gO complex, as it is not found in extracellular virions or secreted from cells. When gO was deleted from a clinical strain of CMV (TR) those viral particles had significantly reduced amounts of gH/gL incorporated into the virion. Additionally, gO deleted from TR virus also inhibited entry into epithelial and endothelial cells, suggesting that gH/gL is also required for epithelial/endothelial cell entry (Wille (2010) J. Virol. 84(5):2585-96). 
     CMV gH/gL can also associate with UL128, UL130, and UL131A (referred to here as UL131) and form a pentameric complex that is required for entry into several cell types, including epithelial cells, endothelial cells, and dendritic cells (Hahn et al (2004) J. Virol. 78(18):10023-33; Wang and Shenk (2005) Proc. Natl. Acad. Sci USA 102(50):18153-8; Gerna et al (2005). J. Gen. Virol. 84(Pt 6):1431-6; Ryckman et al (2008) J. Virol. 82:60-70). In contrast, this complex is not required for infection of fibroblasts. Laboratory hCMV isolates carry mutations in the UL128-UL131 locus, and mutations arise in clinical isolates after only a few passages in cultured fibroblasts (Akter et al (2003) J. Gen. Virol. 84(Pt 5):1117-22). During natural infection, the pentameric complex elicits antibodies that neutralize infection of epithelial cells, endothelial cells (and likely any other cell type where the pentameric complex mediates viral entry) with very high potency (Macagno et al (2010) J. Virol. 84(2):1005-13). It also appears that antibodies to this complex contribute significantly to the ability of human sera to neutralize infection of epithelial cells (Genini et al (2011) J. Clin. Virol. 52(2):113-8). 
     U.S. Pat. No. 5,767,250 discloses methods for making certain CMV protein complexes that contain gH and gL. The complexes are produced by introducing a DNA construct that encodes gH and a DNA construct that encodes gL into a cell so that the gH and gL are co-expressed. 
     WO 2004/076645 describes recombinant DNA molecules that encode CMV proteins. According to this document, combinations of distinct DNA molecules that encode different CMV proteins, can be introduced into cells to cause co-expression of the encoded CMV proteins. When gM and gN were co-expressed in this way, they formed a disulfide-linked complex. Rabbits immunized with DNA constructs that produced the gM/gN complex or with a DNA construct encoding gB produced equivalent neutralizing antibody responses. 
     A need exists for nucleic acids that encode two or more herpes virus proteins, for methods of expressing two or more herpes virus proteins in the same cell, and for immunization methods that produce better immune responses. 
     SUMMARY OF THE INVENTION 
     The invention relates to platforms for co-delivery of two or more herpesvirus proteins, such as cytomegalovirus (CMV) proteins, to cells, particularly proteins that form complexes in vivo. In one aspect, the invention is a recombinant polycistronic nucleic acid molecules that contain a first sequence encoding a first herpesvirus (e.g., CMV) protein or fragment thereof, and a second sequence encoding a second herpesvirus (e.g., CMV) protein or fragment thereof. 
     For example, the invention provides a self-replicating RNA molecule comprising a polynucleotide which comprises a) a first nucleotide sequence encoding a first protein or fragment thereof from a herpes virus; and b) a second nucleotide sequence encoding a second protein or fragment thereof from the herpes virus. The first nucleotide sequence and second nucleotide sequence are operably linked to one or more control elements so that when the self-replicating RNA molecule is introduced into a suitable cell, the first and second herpes virus proteins or fragments thereof are produced in an amount sufficient for the formation of a complex in the cell that contains the first and second proteins or fragments. Preferably, the first protein and the second protein are not the same protein or fragments of the same protein, the first protein is not a fragment of the second protein, and the second protein is not a fragment of the first protein. The first nucleotide sequence can be operably linked to a first control element and the second nucleotide sequence can be operably linked to a second control element. 
     The self-replicating RNA molecule can further comprise a third nucleotide sequence encoding a third protein or fragment thereof from said herpes virus, optionally a fourth nucleotide sequence encoding a fourth protein or fragment thereof from said herpes virus; and optionally a fifth nucleotide sequence encoding a fifth protein or fragment thereof from said herpes virus. When sequences encoding additional proteins or fragments from a herpes virus are present (i.e., the third, fourth and fifth nucleotide sequences) they are operably linked to one or more control elements. In one example of a pentacistronic construct, the first nucleotide sequence is operably linked to a first control element, the second nucleotide sequence is operably linked to a second control element, the third nucleotide sequence is operably linked to a third control element, the fourth nucleotide sequence is operably linked to a fourth control element, and the fifth nucleotide sequence is operably linked to a fifth control element. The control elements present in the construct (e.g., first, second, third, fourth and fifth control elements) can be independently selected from the group consisting of a subgenomic promoter, an IRES, and a viral (e.g., FMDV) 2A site. 
     The herpes virus can be HSV-1, 1, HSV-2, VZV, EBV type 1, EBV type 2, CMV, HHV-6 type A, HHV-6 type B, HHV-7 and HHV-8. In some embodiments, the recombinant polycistronic nucleic acid molecule (e.g., self replicating RNA) encodes gH or a fragment thereof and gL or a fragment thereof of any one of these herpes viruses. In more particular embodiments, the herpes virus is CMV or VZV. 
     When the recombinant polycistronic nucleic acid molecule (e.g., self replicating RNA) encodes two or more VZV proteins, the proteins can be selected from the group consisting of gB, gE, gH, gI, gL and a fragment (e.g., of at least 10 amino acids) thereof. In some embodiments, the recombinant polycistronic nucleic acid molecule (e.g., self replicating RNA) encodes VZV gH or a fragment thereof and VZV gL or a fragment thereof. 
     In a particular example, the invention provides a self-replicating RNA molecule comprising a polynucleotide which comprises a) a first sequence encoding a first cytomegalovirus (CMV) protein or fragment thereof; and b) a second sequence encoding a second CMV protein or fragment thereof. The first sequence and second sequence are operably linked to one or more control elements so that when the self-replicating RNA molecule is introduced into a suitable cell, the first and second CMV proteins are produced in an amount sufficient for the formation of a complex in the cell that contains the first and second CMV proteins or fragments. 
     The first CMV protein and the second CMV protein are independently selected from the group consisting of gB, gH, gL; gO; gM, gN; UL128, UL130, UL131, and a fragment of any one of the foregoing. Preferably, the first CMV protein and the second CMV protein are not the same protein or fragments of the same protein, the first CMV protein is not a fragment of the second CMV protein, and the second CMV protein is not a fragment of the first CMV protein. If desired, the self-replicating RNA molecule can further comprise a third sequence encoding a third CMV protein, wherein the third sequences is operably linked to a control element. Similarly, additional sequences encoding additional CMV proteins (e.g., a fourth sequence encoding a fourth CMV protein, a fifth sequence encoding a fifth CMV protein) can be included. The control elements can be independently selected from the group consisting of a subgenomic promoter, and IRES, and a viral 2A site. 
     In some embodiments, the self replicating nucleic acid molecule encodes the CMV proteins gH and gL. In other embodiments, the self-replicating RNA molecule encodes the CMV proteins gH, gL, and gO. In other embodiments, the self-replicating RNA molecule encodes the CMV proteins gH, gL, UL128, UL130 and UL131. 
     The self replicating RNA molecules can be an alphavirus replicon. In such instances, the alphavirus replicon can be delivered in the form of an alphavirus replicon particle (VRP). The self replicating RNA molecule can also be in the form of a “naked” RNA molecule. 
     The invention also relates to a recombinant DNA molecule that encodes a self replicating RNA molecule as described herein. In some embodiments, the recombinant DNA molecule is a plasmid. In some embodiments, the recombinant DNA molecule includes a mammalian promoter that drive transcription of the encoded self replicating RNA molecule. 
     The invention also relates to compositions that comprise a self-replicating RNA molecule as described herein and a pharmaceutically acceptable vehicle. The self-replicating RNA molecule can be “naked.” In some embodiments, the composition comprises a self-replicating RNA molecule that encodes the CMV proteins gH and gL. In other embodiments, the composition further comprises a self-replicating RNA molecule that encodes the CMV protein gB. The composition can also contain an RNA delivery system such as a liposome, a polymeric nanoparticle, an oil-in-water cationic nanoemulsion or combinations thereof. For example, the self-replicating RNA molecule can be encapsulated in a liposome. 
     In certain embodiments, the composition comprises a VRP that contains a alphavirus replicon that encodes two or more CMV proteins. In some embodiments, the VRP comprises a replicon that encodes CMV gH and gL. If desired, the composition can further comprising a second VRP containing a replicon that encodes CMV gB. The composition can also comprise an adjuvant. 
     The invention also relates to methods of forming a CMV protein complex. In some embodiments a self-replicating RNA encoding two or more CMV proteins is delivered to a cell, the cell is maintained under conditions suitable for expression of the CMV proteins, wherein a CMV protein complex is formed. In other embodiments, a VRP that contains a self-replicating RNA encoding two or more CMV proteins is delivered to a cell, the cell is maintained under conditions suitable for expression of the CMV proteins, wherein a CMV protein complex is formed. The method can be used to form a CMV protein complex in a cell in vivo. 
     The invention also relates to a method for inducing an immune response in an individual. In some embodiments, a self-replicating RNA encoding two or more CMV proteins is administered to the individual. The self-replicating RNA molecule can be administered as a composition that contains an RNA delivery system, such as a liposome. In other embodiments, a VRP that contains a self-replicating RNA encoding two or more CMV proteins is administered to the individual. In preferred embodiments, the self-replicating RNA molecule encodes CMV proteins gH and gL. Preferably, the induced immune response comprises the production of neutralizing anti-CMV antibodies. More preferably, the neutralizing antibodies are complement-independent. 
     The invention also relates to a method of inhibiting CMV entry into a cell comprising contacting the cell with a self-replicating RNA molecule that encodes two or more CMV proteins, such as gH and gL. The cell can be selected from the group consisting of an epithelial cell, an endothelial cell, a fibroblast and combinations thereof. In some embodiments, the cell is contacted with a VRP that contains a self-replicating RNA encoding two or more CMV proteins. 
     The invention also relates to the use of a self-replicating RNA molecule that encodes two or more CMV proteins (e.g., a VRP, a composition comprising the self-replicating RNA molecule and a liposome) form a CMV protein complex in a cell, to induce an immune response or to inhibit CMV entry into a cell. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a schematic of CMV identifying known glycoprotein complexes involved in CMV entry into target cells. Envelope glycoproteins represent attractive vaccine candidates as they are expressed on the viral surface and can elicit protective and long lasting virus-neutralizing humoral immune responses. The structural glycoproteins mediating these processes can be divided into two classes; those that are conserved throughout the herpes virus family and those that are not. Among those that are conserved are gB, gH, gL, gM and gN. Many of these glycoproteins form complexes with one another (gH/gL/±gO; gH/gL/UL128/UL130/UL131; gM/gN) to facilitate localization to the viral surface and to carry out their functions in viral attachment, entry and cell fusion. 
         FIGS. 2A-2F  are schematics of CMV constructs.  FIG. 2A , Schematic of the gB constructs (“gB FL”, full-length gB; soluble gBs “gB sol 750” and “gB sol 692”) described in Example 1. Two different soluble versions of gB were constructed; gB sol 750 lacks the transmembrane spanning domain and cytoplasmic domain, gB sol 692 also lacks a hydrophobic region and is similar to the gB sol described in Reap et al. (2007) Clin. Vacc. Immunol. 14:748-55.  FIG. 2B , Schematic of the gB replicon vectors used to produce viral replicaton particles (VRPs).  FIG. 2C , Schematic of the gH constructs (“gH FL”, full-length gH; soluble gH “gH sol”) described in Example 1. A single soluble version of gH was constructed which lacked the transmembrane spanning domain  FIG. 2D , Schematic of the gH replicon vectors used to produce VRPs.  FIG. 2E , Schematic of gL construct described in Example 1.  FIG. 2F , Schematic of the gL replicon vector used to produce VRPs. In  FIGS. 2B, 2D and 2F , “NSP1,” “NSP2,” “NSP3,” and “NSP4,” are alphavirus nonstructural proteins 1-4, respectively, required for replication of the virus. 
         FIGS. 3A and 3B  show that mice immunized with gB (FL, sol 750, sol 692) or gH (FL, sol) VRPs induced antibody responses that were neutralizing in the presence of guinea pig complement. The neutralization assay was done by pre-incubating the CMV virus strain TB40UL32E-GFP (which encodes the enhanced green fluorescent protein-GFP, Sampaio et al (2005) J. Virol. 79(5):2754-67), with mouse sera and guinea pig complement before infection of ARPE-19 epithelial cells. Five days post-infection, the number of GFP positive cells was determined.  FIG. 3A , Serum dilution curves for all sera analyzed in ARPE-19 cells in the presence of complement.  FIG. 3B , 50% neutralization titers for the sera samples. Virus incubated with pre-immune sera yielded low neutralization at low dilutions (1:40-1:80). gB (FL, sol 750, sol 692) sera had very strong neutralizing activity with 50% neutralization titers between 1:1800-1:2100. All gB immunized mice yielded a similar neutralization profile. gH (FL, sol) sera had neutralizing activity with 50% neutralization titers around 1:160. See Example 1. 
         FIG. 4A  is a schematic illustration of monocistronic replicons encoding green fluorescent protein (GFP) or red fluorescent protein (mCherry) and a bicistronic replicon encoding GFP and mCherry. “NSP1,” “NSP2,” “NSP3,” and “NSP4,” are alphavirus nonstructural proteins 1-4, respectively. The polycistronic alphavirus replicon system was designed by making modifications to the existing alphavirus replicon system to accommodate multiple subgenomic promoters driving genes of interest. 
         FIG. 4B  are fluorescence plots showing FACS analysis of BHKV cells infected with VRPs containing mono- and bicistronic RNAs. Polycistronic alphavirus VRPs yield more cells expressing both genes of interest at approximately equal amounts (GFP and mCherry; 72.48%) than co-infection of GFP VRP+mCherry VRP (26.30%). See Example 2. 
         FIG. 5A  is a schematic illustration of construction of polycistronic alphavirus replicon constructs encoding gH/gL and gH/gL/gO. 
         FIG. 5B  show that gH/gL form a complex in vitro. VRPs containing replicons encoding gH, gL, gO, gH/gL or gH/gL/gO were produced in BHKV cells. The resulting VRPs were used to infect ARPE-19 cells to demonstrate complex formation in vitro. The alphavirus infected ARPE-19 cells were harvested and analyzed for the presence of gH and gL. ARPE-19 cells infected with VRPs encoding gH/gL produced disulfide linked complexes of gH/gL (see in the absence of DTT, heat). gO did not detectably alter the gH/gL association. The left hand blot shows expression of gH protein. The right hand blot shows expression of gL protein. Molecular weight markers are indicated between the blots. •=monomeric gH, ••=monomeric gL, &lt;=herodimer (gH+gL), *=dimer of heterodimers. 
         FIG. 5C  shows immunoprecipitation of gH and gH/gL complexes from BHKV cells infected with VRPs Immunoprecipitation was performed using mouse IgG antibodies as a control (Lanes 2, 4, 7, and 10) or mouse anti-gH antibodies (Genway) to immunoprecipitate gH (Lanes 3, 5, 8, and 11). Western blots were performed using pooled rabbit anti-gL antibody and rabbit anti-gH antibody. Lanes 1, 6, and 9 show gH protein (upper band ˜75 kDa) and gL protein (lower band ˜30 kDa) for reference. Lanes 2 and 3 are lysates infected with gH-VRP. Lane 2 shows that the control antibody did not immunoprecipitate gH. Lane 3 shows the anti-gH antibody immunoprecipitated gH. Lanes 4 and 5 are from lysates infected with gL-VRP only. No gH protein was immunoprecipitated. Lanes 7 and 8 are from lysates infected with bicistronic gH/gL-VRP. Lane 8 shows that gL was immunoprecipitated using the gH antibody. (See asterisk). Lanes 10 and 11 are from lysates infected with tricistronic gH/gL/gO-VRP. Lane 11 shows that gL was immunoprecipitated using the gH antibody. (See asterisk). Molecular Weight markers are also shown (MW). See Example 3. 
         FIGS. 6A-6C  shows that VRPs that affect gH/gL complex formation in vitro induce potent immune response to CMV which is qualitatively and quantitatively superior to the response to gB VRPs.  FIG. 6A  and  FIG. 6B  show serum dilution curves for gH, gL, gO, gH+gL, gH+gL+gO, gH/gL and gH/gL/gO VRP-immunized mice in neutralization of TB40-UL32-EGFP infection of ARPE-19 cells in the presence ( FIG. 6A ) or absence ( FIG. 6B ) of complement. Various dilutions of sera were pre-incubated with TB40UL32E-GFP in the presence or absence of guinea pig complement and then added to ARPE-19 epithelial cells. After 5 day infection with the virus, GFP-positive cells were counted.  FIG. 6C  is a graph showing 50% neutralization titers obtained in the presence and absence of complement. “3wp3,” three weeks post-third immunization. VRPs expressing single CMV proteins (gH, gL, gO VRPs or co-administered gH, gL and gO VRPs) did not enhance neutralizing activity beyond that of gH alone. In contrast, sera from mice immunized with bicistronic gH/gL or tricistronic gH/gL/gO VRPs demonstrated potent neutralizing responses. Moreover, the potent neutralizing responses were similar in the presence and absence of guinea pig complement, showing that polycistronic VRPs successfully induced a complement-independent immune response. See Example 4. 
         FIGS. 7A and 7B  shows that VRPs that affect gH/gL complex formation in vitro induced antibodies that potently neutralized infection of MRC-5 fibroblast cells.  FIG. 7A  shows serum dilution curves for gH, gL, gO, gH+gL, gH+gL+gO, gH/gL and gH/gL/gO VRP-immunized mice in MRC-5 cells in the absence of complement. Various dilutions of sera were pre-incubated with TB40GFP in the presence or absence of guinea pig complement and then added to MRC-5 fibroblast cells. After 5 day infection with the virus, GFP-positive cells were counted.  FIG. 7B  is a graph showing 50% neutralization titers obtained in a MRC-5 fibroblast cell model in the absence of complement. “3wp3,” three weeks post-third immunization. VRPs expressing single CMV proteins (gH, gL, gO VRPs or co-administered gH, gL and gO VRPs) did not enhance neutralizing activity beyond that of gH alone. In contrast, sera from mice immunized with bicistronic gH/gL or tricistronic gH/gL/gO VRPs demonstrated extremely potent neutralizing responses. See Example 4. 
         FIGS. 8A and 8B  are graphs showing that the neutralizing antibodies induced by delivery of the polycistronic VRPs were cross-neutralizing antibodies. The sera from mice immunized with gH/gL and gH/gL/gO VRPs were able to neutralize TB40UL32E-GFP and VR1814 clinical strains of CMV in both ARPE-19 epithelial cells ( FIG. 8A ) and MRC-5 fibroblast cells ( FIG. 8B ) in the absence of guinea pig complement in an IE-1 neutralization assay. 
         FIG. 9  is a graph showing that the neutralizing antibodies elicited against gH FL/gL are complement-independent and similar to natural immunity in titer. Mice were immunized with gB FL or gH FL/gL VRPs at 1×10 6  IU, 3 times, 3 weeks apart before the terminal bleed. Sera was analyzed for ability to neutralize TB40UL32E-EGFP CMV infection of ARPE-19 cells in the presence and absence of guinea pig complement in a neutralization assay. Unlike antibodies elicited by gB, antibodies elicited by gH FL/gL are complement-independent. Furthermore, gH FL/gL antibodies in these vaccinated mice were similar in titer to those found in naturally infected human subjects. 
         FIG. 10  shows a plasmid map for pVCR modified gH-SGPgL-SGPgO. 
         FIG. 11  show a plasmid map for pVCR modified gH-SGPgL. 
         FIG. 12  show a plasmid map for pVCR modified gH sol-SGPgL. 
         FIG. 13  show a plasmid map for pVCR modified gH sol-SGPgL-SGPgO. 
         FIG. 14A-14H  show the nucleotide sequence (SEQ ID NO: 83) of the plasmid encoding the A160 self-replicating RNA molecule which encodes CMV surface glycoprotein H (gH) and CMV surface glycoprotein L (gL). The nucleotide sequences encoding gH and gL are underlined. 
         FIG. 15A-15H  show the nucleotide sequence (SEQ ID NO: 84) of the plasmid encoding the A322 self-replicating RNA molecule which encodes the soluble form of CMV surface glycoprotein H (gHsol) and CMV surface glycoprotein L (gL). The nucleotide sequences encoding gHsol and gL are underlined. 
         FIG. 16A-16H  show the nucleotide sequence (SEQ ID NO: 85) of the plasmid encoding the A323 self-replicating RNA molecule which encodes CMV surface glycoprotein B (gB). The nucleotide sequence encoding gB is underlined. 
         FIGS. 17A and 17B  are histograms showing 50% neutralizing titers of sera from mice that were immunized with VRP or self-replicating RNA.  FIG. 17A  shows 50% neutralizing titers against human CMV strain TB40UL32E-EGFP (“TB40) on ARPE-19 cells, and  FIG. 17B  shows 50% neutralizing titers against human CMV strain 8819 on ARPE-19 cells 
         FIG. 18  is a schematic of petacistronic RNA replicons, A526 (SEQ ID NO:56), A527 (SEQ ID NO:57), A554 (SEQ ID NO:65), A555 (SEQ ID NO:66) and A556 (SEQ ID NO:67), that encode five CMV proteins. Subgenomic promoters are shown by arrows, other control elements are labeled. 
         FIG. 19  is a fluorescence histogram showing that BHKV cells transfected with the A527 RNA replicon express the gH/gL/UL128/UL130/UL131 pentameric complex. Cell stain was performed using antibodies that bind a conformational epitope present on the pentameric complex (Macagno (2010) J. Virol. 84(2):1005-13). 
         FIG. 20  is a schematic and graph. The schematic shows bicistronic RNA replicons, A160 and A531-A537, that encode CMV gH and gL. The graph shows neutralizing activity of immune sera from mice immunized with VRPs that contained the replicons. 
         FIG. 21  is a graph showing anti-VZV protein antibody response in immune sera from mice immunized with monocistronic RNA replicons that encoded VZV proteins or bicistronic RNA replicons that encoded VZV gE and gI, or gH and gL. The mice were immunized with 7 μg RNA formulated with a CNE (see, Example 7). 
         FIG. 22  is a graph showing anti-VZV protein antibody response in immune sera from mice immunized with monocistronic RNA replicons that encoded VZV proteins or bicistronic RNA replicons that encoded VZV gE and gI, or gH and gL. The mice were immunized with 1 μg RNA formulated with a CNE (see, Example 7). 
     
    
    
     DETAILED DESCRIPTION 
     The invention provides platforms for co-delivery of herpesvirus proteins, such as cytomegalovirus (CMV) proteins, to cells, particularly proteins that form complexes in vivo. In some embodiments, these proteins and the complexes they form elicit potent neutralizing antibodies. The immune response produced by co-delivery of herpesvirus (e.g., CMV) proteins, particularly those that form complexes in vivo (e.g., gH/gL), can be superior to the immune response produced using other approaches. For example, an RNA molecule (e.g., a replicon) that encodes both gH and gL of CMV can induce better neutralizing titers and/or protective immunity in comparison to an RNA molecule that encodes gB, an RNA molecule that encodes gH, an RNA molecule that encodes gL, or even a mixture of RNA molecules that individually encode gH or gL. Further, a replicon encoding gH/gL/UL128/UL130/UL131 can provide responses superior to those encoding only gH/gL. 
     In a general aspect, the invention relates to platforms for delivery of two or more herpesvirus (e.g., CMV) proteins to cells. The platforms comprise recombinant polycistronic nucleic acid molecules that contain a first sequence encoding a first herpesvirus (e.g., CMV) protein or fragment thereof, and a second sequence encoding a second herpesvirus (e.g., CMV) protein or fragment thereof. If desired, one or more additional sequences encoding additional proteins, for example, a third herpesvirus (e.g., CMV) protein or fragment thereof, a fourth herpesvirus (e.g., CMV) protein or fragment thereof, a fifth herpesvirus (e.g., CMV) protein or fragment thereof etc. can be present in the recombinant polycistronic nucleic acid molecule. The sequences encoding herpesvirus (e.g., CMV) proteins or fragments thereof are operably linked to one or more suitable control elements so that the herpesvirus (e.g., CMV) proteins or fragments are produced by a cell that contains the recombinant polycistronic nucleic acid. 
     In the polycistronic nucleic acids described herein, the encoded first and second herpesvirus proteins or fragments, and the encoded third, forth and fifth herpes virus proteins or fragments, if present, generally and preferably are from the same herpes virus. In certain examples, all herpes virus proteins or fragments encoded by a polycistronic vector are CMV proteins or VZV proteins. 
     The recombinant polycistronic nucleic acid molecules described herein provide the advantage of delivering sequences that encode two or more herpesvirus (e.g., CMV) proteins to a cell, and driving the expression of the herpesvirus (e.g., CMV) proteins at sufficient levels to result in the formation of a protein complex containing the two or more herpesvirus (e.g., CMV) proteins in vivo. Using this approach, the two or more encoded herpesvirus (e.g., CMV) proteins can be expressed at sufficient intracellular levels for the formation of herpesvirus (e.g., CMV) protein complexes (e.g., gH/gL). For example, the encoded herpesvirus (e.g., CMV) proteins or fragments thereof can be expressed at substantially the same level, or if desired, at different levels by selecting appropriate expression control sequences (e.g., promoters, IRES, 2A site etc.). This is significantly more efficient way to produce protein complexes in vivo than by co-delivering two or more individual DNA molecules that encode different herpesvirus (e.g., CMV) to the same cell, which can be inefficient and highly variable. See, e.g., WO 2004/076645. 
     The recombinant polycistronic nucleic acid molecule can be based on any desired nucleic acid such as DNA (e.g., plasmid or viral DNA) or RNA. Any suitable DNA or RNA can be used as the nucleic acid vector that carries the open reading frames that encode herpesvirus (e.g., CMV) proteins or fragments thereof. Suitable nucleic acid vectors have the capacity to carry and drive expression of more than one protein gene. Such nucleic acid vectors are known in the art and include, for example, plasmids, DNA obtained from DNA viruses such as vaccinia virus vectors (e.g., NYVAC, see U.S. Pat. No. 5,494,807), and poxvirus vectors (e.g., ALVAC canarypox vector, Sanofi Pasteur), and RNA obtained from suitable RNA viruses such as an alphavirus. If desired, the recombinant polycistronic nucleic acid molecule can be modified, e.g., contain modified nucleobases and or linkages as described further herein. Preferably, the polycistronic nucleic acid molecule is an RNA molecule. 
     In some aspects, the recombinant polycistronic nucleic acid molecule is a DNA molecule such as plasmid DNA. Such DNA molecules can, for example, encode a polycistronic replicon and contain a mammalian promoter that drives transcription of the replicon. Recombinant polycistronic nucleic acid molecules or this type can be administered to a mammal and then be transcribed in situ to produce a polycistronic replicon that expresses herpesvirus proteins. 
     In some aspects, the invention is a polycistronic nucleic acid molecule that contains a sequence encoding a herpesvirus gH or fragment thereof, and a herpesvirus gL or a fragment thereof. The gH and gL proteins, or fragments thereof, can be from any desired herpes virus such as HSV-1, HSV-2, VZV, EBV type 1, EBV type 2, CMV, HHV-6 type A, HHV-6 type B, HHV-7, KSHV, and the like. Preferably, the herpesvirus is VZV, HSV-2, HSV-1, EBV (type 1 or type 2) or CMV. More preferably, the herpesvirus is VZV, HSV-2 or CMV. Even more preferably, the herpesvirus is CMV. The sequences of gH and gL proteins and of nucleic acids that encode the proteins from these viruses are well known in the art. Exemplary sequences are identified in Table 1. The polycistronic nucleic acid molecule can contain a first sequence encoding a gH protein disclosed in Table 1, or a fragment thereof, or a sequence that is at least about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical thereto. The polycistronic nucleic acid molecule can also contain a second sequence encoding a gL protein disclosed in Table 1, or a fragment thereof, or a sequence that is at least about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical thereto. 
     
       
         
           
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Virus 
                 gH accession number 
                 gL accession number 
               
               
                   
               
             
            
               
                 HSV-1 (HHV-1) 
                 NP_044623.1 
                 NP_044602.1 
               
               
                 HSV-2 (HHV-2) 
                 NP_044491.1 
                 NP_044470.1 
               
               
                 VZV (HHV-3) 
                 NP_040160.1 
                 NP_040182.1 
               
               
                 EBV type 1 (HHV-4) 
                 YP_401700.1 
                 YP_401678.1 
               
               
                 EBV type 2 (HHV-4) 
                 YP_001129496.1 
                 YP_001129472.1 
               
               
                 CMV (HHV-5) 
                 YP_081523.1 
                 YP_081555.1 
               
               
                 HHV-6 type A 
                 NP_042941.1 
                 NP_042975.1 
               
               
                 HHV-6 type B 
                 NP_050229.1 
                 NP_050261.1 
               
               
                 HHV-7 
                 YP_073788.1 
                 YP_073820.1 
               
               
                 KSHV (HHV-8) 
                 YP_001129375.1 
                 YP_001129399.1 
               
               
                   
               
            
           
         
       
     
     In this description of the invention, to facilitate a clear description of the nucleic acids, particular sequence components are referred to as a “first sequence,” a “second sequence,” etc. It is to be understood that the first and second sequences can appear in any desired order or orientation, and that no particular order or orientation is intended by the words “first”, “second” etc. Similarly, protein complexes are referred to by listing the proteins that are present in the complex, e.g., gH/gL. This is intended to describe the complex by the proteins that are present in the complex and does not indicate relative amounts of the proteins or the order or orientation of sequences that encode the proteins on a recombinant nucleic acid. 
     Certain preferred embodiments, such as alphavirus VRP and self-replicating RNA that contain sequences encoding CMV proteins, are further described herein. It is intended that the sequences encoding CMV proteins in such preferred embodiments, can be replaced with sequences encoding proteins, such as gH and gL from other herpesviruses. 
     Alphavirus VRP Platforms 
     In some embodiments, CMV proteins are delivered to a cell using alphavirus replicon particles (VRP) which employ polycistronic replicons (or vectors) as described below. As used herein, “polycistronic” includes bicistronic vectors as well as vectors comprising three or more cistrons. Cistrons in a polycistronic vector can encode CMV proteins from the same CMV strains or from different CMV strains. The cistrons can be oriented in any 5′-3′ order. Any nucleotide sequence encoding a CMV protein can be used to produce the protein. Exemplary sequences useful for preparing the polycistronic nucleic acids that encode two or more CMV proteins or fragments thereof are described herein. 
     As used herein, the term “alphavirus” has its conventional meaning in the art and includes various species such as Venezuelan equine encephalitis virus (VEE; e.g., Trinidad donkey, TC83CR, etc.), Semliki Forest virus (SFV), Sindbis virus, Ross River virus, Western equine encephalitis virus, Eastern equine encephalitis virus, Chikungunya virus, S.A. AR86 virus, Everglades virus, Mucambo virus, Barmah Forest virus, Middelburg virus, Pixuna virus, O′nyong-nyong virus, Getah virus, Sagiyama virus, Bebaru virus, Mayaro virus, Una virus, Aura virus, Whataroa virus, Banbanki virus, Kyzylagach virus, Highlands J virus, Fort Morgan virus, Ndumu virus, and Buggy Creek virus. The term alphavirus may also include chimeric alphaviruses (e.g., as described by Perri et al., (2003) J. Virol. 77(19):10394-403) that contain genome sequences from more than one alphavirus. 
     An “alphavirus replicon particle” (VRP) or “replicon particle” is an alphavirus replicon packaged with alphavirus structural proteins. 
     An “alphavirus replicon” (or “replicon”) is an RNA molecule which can direct its own amplification in vivo in a target cell. The replicon encodes the polymerase(s) which catalyze RNA amplification (nsP1, nsP2, nsP3, nsP4) and contains cis RNA sequences required for replication which are recognized and utilized by the encoded polymerase(s). An alphavirus replicon typically contains the following ordered elements: 5′ viral sequences required in cis for replication, sequences which encode biologically active alphavirus nonstructural proteins (nsP1, nsP2, nsP3, nsP4), 3′ viral sequences required in cis for replication, and a polyadenylate tract. An alphavirus replicon also may contain one or more viral subgenomic “junction region” promoters directing the expression of heterologous nucleotide sequences, which may, in certain embodiments, be modified in order to increase or reduce viral transcription of the subgenomic fragment and heterologous sequence(s) to be expressed. Other control elements can be used, as described below. 
     Alphavirus replicons encoding CMV proteins are used to produce VRPs. Such alphavirus replicons comprise sequences encoding at least two CMV proteins or fragments thereof. These sequences are operably linked to one or more suitable control elements, such as a subgenomic promoter, an IRES (e.g., EMCV, EV71), and a viral 2A site, which can be the same or different. Delivery of components of these complexes using the polycistronic vectors disclosed herein is an efficient way of providing nucleic acid sequences that encode two or more CMV proteins in desired relative amounts; whereas if multiple alphavirus constructs were used to deliver individual CMV proteins for complex formation, efficient co-delivery of VRPs would be required. 
     Any combination of suitable control elements can be used in any order. In one example, a single subgenomic promoter is operable linked to two sequences encoding two different CMV proteins, and an IRES is positioned between the two coding sequences. In another example, two sequences that encode two different CMV proteins are operably linked to separate promoters. In still another example, the two sequences that encode two different CMV proteins are operably linked to a single promoter. The two sequences that encode two different CMV proteins are linked to each other through a nucleotide sequence encoding a viral 2A site, and thus encode a single amino acid chain that contain the amino acid sequences of both CMV proteins. The viral 2A site in this context is used to generate two CMV proteins from encoded polyprotein. 
     Subgenomic Promoters 
     Subgenomic promoters, also known as junction region promoters can be used to regulate protein expression. Alphaviral subgenomic promoters regulate expression of alphaviral structural proteins. See Strauss and Strauss, “The alphaviruses: gene expression, replication, and evolution,” Microbiol Rev. 1994 September; 58(3):491-562. A polycistronic polynucleotide can comprise a subgenomic promoter from any alphavirus. When two or more subgenomic promoters are present in a polycistronic polynucleotide, the promoters can be the same or different. For example, the subgenomic promoter can have the sequence CTCTCTACGGCTAACCTGAATGGA (SEQ ID NO: 1). In certain embodiments, subgenomic promoters can be modified in order to increase or reduce viral transcription of the proteins. See U.S. Pat. No. 6,592,874. 
     Internal Ribosomal Entry Site (IRES) 
     In some embodiments, one or more control elements is an internal ribosomal entry site (IRES). An IRES allows multiple proteins to be made from a single mRNA transcript as ribosomes bind to each IRES and initiate translation in the absence of a 5′-cap, which is normally required to initiate translation of protein in eukaryotic cells. For example, the IRES can be EV71 (SEQ ID NO:50) or EMCV (SEQ ID NO:49). 
     Viral 2A Site 
     The FMDV 2A protein is a short peptide that serves to separate the structural proteins of FMDV from a nonstructural protein (FMDV 2B). Early work on this peptide suggested that it acts as an autocatalytic protease, but other work (e.g., Donnelly et al., (2001), J. Gen. Virol. 82, 1013-1025) suggest that this short sequence and the following single amino acid of FMDV 2B (Gly) acts as a translational stop-start. Regardless of the precise mode of action, the sequence can be inserted between two polypeptides, and effect the production of multiple individual polypeptides from a single open reading frame. In the context of this invention, FMDV 2A sequences can be inserted between the sequences encoding at least two CMV proteins, allowing for their synthesis as part of a single open reading frame. For example, the open reading frame may encode a gH protein and a gL protein separated by a sequence encoding a viral 2A site. A single mRNA is transcribed then, during the translation step, the gH and gL peptides are produced separately due to the activity of the viral 2A site. Any suitable viral 2A sequence may be used. Often, a viral 2A site comprises the consensus sequence Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro, where X is any amino acid (SEQ ID NO: 2). For example, the Foot and Mouth Disease Virus 2A peptide sequence is DVESNPGP (SEQ ID NO: 3). See Trichas et al., “Use of the viral 2A peptide for bicistronic expression in transgenic mice,” BMC Biol. 2008 Sep. 15; 6:40, and Halpin et al., “Self-processing 2A-polyproteins—a system for co-ordinate expression of multiple proteins in transgenic plants,” Plant J. 1999 February; 17(4):453-9. 
     In some embodiments an alphavirus replicon is a chimeric replicon, such as a VEE-Sindbis chimeric replicon (VCR) or a VEE strain TC83 replicon (TC83R) or a TC83-Sindbis chimeric replicon (TC83CR). In some embodiments a VCR contains the packaging signal and 3′ UTR from a Sindbis replicon in place of sequences in nsP3 and at the 3′ end of the VEE replicon; see Perri et al.,  J. Virol.  77, 10394-403, 2003. In some embodiments, a TC83CR contains the packaging signal and 3′ UTR from a Sindbis replicon in place of sequences in nsP3 and at the 3′ end of aVEE strain TC83replicon. 
     Producing VRPs 
     Methods of preparing VRPs are well known in the art. In some embodiments an alphavirus is assembled into a VRP using a packaging cell. An “alphavirus packaging cell” (or “packaging cell”) is a cell that contains one or more alphavirus structural protein expression cassettes and that produces recombinant alphavirus particles after introduction of an alphavirus replicon, eukaryotic layered vector initiation system (e.g., U.S. Pat. No. 5,814,482), or recombinant alphavirus particle. The one or more different alphavirus structural protein cassettes serve as “helpers” by providing the alphavirus structural proteins. An “alphavirus structural protein cassette” is an expression cassette that encodes one or more alphavirus structural proteins and comprises at least one and up to five copies (i.e., 1, 2, 3, 4, or 5) of an alphavirus replicase recognition sequence. Structural protein expression cassettes typically comprise, from 5′ to 3′, a 5′ sequence which initiates transcription of alphavirus RNA, an optional alphavirus subgenomic region promoter, a nucleotide sequence encoding the alphavirus structural protein, a 3′ untranslated region (which also directs RNA transcription), and a polyA tract. See, e.g., WO 2010/019437. 
     In preferred embodiments two different alphavirus structural protein cassettes (“split” defective helpers) are used in a packaging cell to minimize recombination events which could produce a replication-competent virus. In some embodiments an alphavirus structural protein cassette encodes the capsid protein (C) but not either of the glycoproteins (E2 and E1). In some embodiments an alphavirus structural protein cassette encodes the capsid protein and either the E1 or E2 glycoproteins (but not both). In some embodiments an alphavirus structural protein cassette encodes the E2 and E1 glycoproteins but not the capsid protein. In some embodiments an alphavirus structural protein cassette encodes the E1 or E2 glycoprotein (but not both) and not the capsid protein. 
     In some embodiments, VRPs are produced by the simultaneous introduction of replicons and helper RNAs into cells of various sources. Under these conditions, for example, BHKV cells (1×10 7 ) are electroporated at, for example, 220 volts, 1000 μF, 2 manually pulses with 10 μg replicon RNA:6 μg defective helper Cap RNA:10 μg defective helper Gly RNA, alphavirus containing supernatant is collected ˜24 hours later. Replicons and/or helpers can also be introduced in DNA forms which launch suitable RNAs within the transfected cells. 
     A packaging cell may be a mammalian cell or a non-mammalian cell, such as an insect (e.g., SF9) or avian cell (e.g., a primary chick or duck fibroblast or fibroblast cell line). See U.S. Pat. No. 7,445,924. Avian sources of cells include, but are not limited to, avian embryonic stem cells such as EB66® (VIVALIS); chicken cells, including chicken embryonic stem cells such as EBx® cells, chicken embryonic fibroblasts, and chicken embryonic germ cells; duck cells such as the AGE1.CR and AGE1.CR.pIX cell lines (ProBioGen) which are described, for example, in Vaccine 27:4975-4982 (2009) and WO2005/042728); and geese cells. In some embodiments, a packaging cell is a primary duck fibroblast or duck retinal cell line, such as AGE.CR (PROBIOGEN). 
     Mammalian sources of cells for simultaneous nucleic acid introduction and/or packaging cells include, but are not limited to, human or non-human primate cells, including PerC6 (PER.C6) cells (CRUCELL N.V.), which are described, for example, in WO 01/38362 and WO 02/40665, as well as deposited under ECACC deposit number 96022940); MRC-5 (ATCC CCL-171); WI-38 (ATCC CCL-75); fetal rhesus lung cells (ATCC CL-160); human embryonic kidney cells (e.g., 293 cells, typically transformed by sheared adenovirus type 5 DNA); VERO cells from monkey kidneys); cells of horse, cow (e.g., MDBK cells), sheep, dog (e.g., MDCK cells from dog kidneys, ATCC CCL34 MDCK (NBL2) or MDCK 33016, deposit number DSM ACC 2219 as described in WO 97/37001); cat, and rodent (e.g., hamster cells such as BHK21-F, HKCC cells, or Chinese hamster ovary (CHO) cells), and may be obtained from a wide variety of developmental stages, including for example, adult, neonatal, fetal, and embryo. 
     In some embodiments a packaging cell is stably transformed with one or more structural protein expression cassette(s). Structural protein expression cassettes can be introduced into cells using standard recombinant DNA techniques, including transferrin-polycation-mediated DNA transfer, transfection with naked or encapsulated nucleic acids, liposome-mediated cellular fusion, intracellular transportation of DNA-coated latex beads, protoplast fusion, viral infection, electroporation, “gene gun” methods, and DEAE- or calcium phosphate-mediated transfection. Structural protein expression cassettes typically are introduced into a host cell as DNA molecules, but can also be introduced as in vitro-transcribed RNA. Each expression cassette can be introduced separately or substantially simultaneously. 
     In some embodiments, stable alphavirus packaging cell lines are used to produce recombinant alphavirus particles. These are alphavirus-permissive cells comprising DNA cassettes expressing the defective helper RNA stably integrated into their genomes. See Polo et al.,  Proc. Natl. Acad. Sci. USA  96, 4598-603, 1999. The helper RNAs are constitutively expressed but the alphavirus structural proteins are not, because the genes are under the control of an alphavirus subgenomic promoter (Polo et al., 1999). Upon introduction of an alphavirus replicon into the genome of a packaging cell by transfection or VRP infection, replicase enzymes are produced and trigger expression of the capsid and glycoprotein genes on the helper RNAs, and output VRPs are produced. Introduction of the replicon can be accomplished by a variety of methods, including both transfection and infection with a seed stock of alphavirus replicon particles. The packaging cell is then incubated under conditions and for a time sufficient to produce packaged alphavirus replicon particles in the culture supernatant. 
     Thus, packaging cells allow VRPs to act as self-propagating viruses. This technology allows VRPs to be produced in much the same manner, and using the same equipment, as that used for live attenuated vaccines or other viral vectors that have producer cell lines available, such as replication-incompetent adenovirus vectors grown in cells expressing the adenovirus E1A and E1B genes. 
     In some embodiments, a two-step process is used: the first step comprises producing a seed stock of alphavirus replicon particles by transfecting a packaging cell with a replicon RNA or plasmid DNA-based replicon. A much larger stock of replicon particles is then produced in a second step, by infecting a fresh culture of packaging cells with the seed stock. This infection can be performed using various multiplicities of infection (MOI), including a MOI=0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1.0, 3, 5, 10 or 20. In some embodiments infection is performed at a low MOI (e.g., less than 1). Over time, replicon particles can be harvested from packaging cells infected with the seed stock. In some embodiments, replicon particles can then be passaged in yet larger cultures of naive packaging cells by repeated low-multiplicity infection, resulting in commercial scale preparations with the same high titer. 
     Self-Replicating RNA Platforms 
     Two or more CMV proteins can be produced by expression of recombinant nucleic acids that encode the proteins in the cells of a subject. Preferably, the recombinant nucleic acid molecules encode two or more CMV proteins, e.g., are polycistronic. As defined above, “polycistronic” includes bicistronic. Preferred nucleic acids that can be administered to a subject to cause the production of CMV proteins are self-replicating RNA molecules. The self-replicating RNA molecules of the invention are based on the genomic RNA of RNA viruses, but lack the genes encoding one or more structural proteins. The self-replicating RNA molecules are capable of being translated to produce non-structural proteins of the RNA virus and CMV proteins encoded by the self-replicating RNA. 
     The self-replicating RNA generally contains at least one or more genes selected from the group consisting of viral replicase, viral proteases, viral helicases and other nonstructural viral proteins, and also comprise 5′- and 3′-end cis-active replication sequences, and a heterologous sequences that encodes two or more desired CMV proteins. A subgenomic promoter that directs expression of the heterologous sequence(s) can be included in the self-replicating RNA. If desired, a heterologous sequence may be fused in frame to other coding regions in the self-replicating RNA and/or may be under the control of an internal ribosome entry site (IRES). 
     Self-replicating RNA molecules of the invention can be designed so that the self-replicating RNA molecule cannot induce production of infectious viral particles. This can be achieved, for example, by omitting one or more viral genes encoding structural proteins that are necessary for the production of viral particles in the self-replicating RNA. For example, when the self-replicating RNA molecule is based on an alpha virus, such as Sindbis virus (SIN), Semliki forest virus and Venezuelan equine encephalitis virus (VEE), one or more genes encoding viral structural proteins, such as capsid and/or envelope glycoproteins, can be omitted. If desired, self-replicating RNA molecules of the invention can be designed to induce production of infectious viral particles that are attenuated or virulent, or to produce viral particles that are capable of a single round of subsequent infection. 
     A self-replicating RNA molecule can, when delivered to a vertebrate cell even without any proteins, lead to the production of multiple daughter RNAs by transcription from itself (or from an antisense copy of itself). The self-replicating RNA can be directly translated after delivery to a cell, and this translation provides a RNA-dependent RNA polymerase which then produces transcripts from the delivered RNA. Thus the delivered RNA leads to the production of multiple daughter RNAs. These transcripts are antisense relative to the delivered RNA and may be translated themselves to provide in situ expression of encoded CMV protein, or may be transcribed to provide further transcripts with the same sense as the delivered RNA which are translated to provide in situ expression of the encoded CMV protein(s). 
     One suitable system for achieving self-replication is to use an alphavirus-based RNA replicon, such as an alphavirus replicon as described herein. These +stranded replicons are translated after delivery to a cell to produce a replicase (or replicase-transcriptase). The replicase is translated as a polyprotein which auto cleaves to provide a replication complex which creates genomic −strand copies of the +strand delivered RNA. These −strand transcripts can themselves be transcribed to give further copies of the +stranded parent RNA and also to give rise to one or more subgenomic transcript which encodes two or more CMV proteins. Translation of the subgenomic transcript thus leads to in situ expression of the CMV protein(s) by the infected cell. Suitable alphavirus replicons can use a replicase from a Sindbis virus, a Semliki forest virus, an eastern equine encephalitis virus, a Venezuelan equine encephalitis virus, etc. 
     A preferred self-replicating RNA molecule thus encodes (i) a RNA-dependent RNA polymerase which can transcribe RNA from the self-replicating RNA molecule and (ii) two or more CMV proteins or fragments thereof. The polymerase can be an alphavirus replicase e.g. comprising alphavirus protein nsP4. Protein nsP4 is the key catalytic component of the replicase. 
     Whereas natural alphavirus genomes encode structural virion proteins in addition to the non-structural replicase polyprotein, it is preferred that an alphavirus based self-replicating RNA molecule of the invention does not encode all alphavirus structural proteins. Thus the self replicating RNA can lead to the production of genomic RNA copies of itself in a cell, but not to the production of RNA-containing alphavirus virions. The inability to produce these virions means that, unlike a wild-type alphavirus, the self-replicating RNA molecule cannot perpetuate itself in infectious form. The alphavirus structural proteins which are necessary for perpetuation in wild-type viruses are absent from self replicating RNAs of the invention and their place is taken by gene(s) encoding the desired gene product (CMV protein or fragment thereof), such that the subgenomic transcript encodes the desired gene product rather than the structural alphavirus virion proteins. 
     Thus a self-replicating RNA molecule useful with the invention have two sequences that encode different CMV proteins or fragments thereof. The sequences encoding the CMV proteins or fragments can be in any desired orientation, and can be operably linked to the same or separate promoters. If desired, the sequences encoding the CMV proteins or fragments can be part of a single open reading frame. In some embodiments the RNA may have one or more additional (downstream) sequences or open reading frames e.g. that encode other additional CMV proteins or fragments thereof. A self-replicating RNA molecule can have a 5′ sequence which is compatible with the encoded replicase. 
     In one aspect, the self-replicating RNA molecule is derived from or based on an alphavirus, such as an alphavirus replicon as defined herein. In other aspects, the self-replicating RNA molecule is derived from or based on a virus other than an alphavirus, preferably, a positive-stranded RNA viruses, and more preferably a picornavirus, flavivirus, rubivirus, pestivirus, hepacivirus, calicivirus, or coronavirus. Suitable wild-type alphavirus sequences are well-known and are available from sequence depositories, such as the American Type Culture Collection, Rockville, Md. Representative examples of suitable alphaviruses include Aura (ATCC VR-368), Bebaru virus (ATCC VR-600, ATCC VR-1240), Cabassou (ATCC VR-922), Chikungunya virus (ATCC VR-64, ATCC VR-1241), Eastern equine encephalomyelitis virus (ATCC VR-65, ATCC VR-1242), Fort Morgan (ATCC VR-924), Getah virus (ATCC VR-369, ATCC VR-1243), Kyzylagach (ATCC VR-927), Mayaro virus (ATCC VR-66; ATCC VR-1277), Middleburg (ATCC VR-370), Mucambo virus (ATCC VR-580, ATCC VR-1244), Ndumu (ATCC VR-371), Pixuna virus (ATCC VR-372, ATCC VR-1245), Ross River virus (ATCC VR-373, ATCC VR-1246), Semliki Forest (ATCC VR-67, ATCC VR-1247), Sindbis virus (ATCC VR-68, ATCC VR-1248), Tonate (ATCC VR-925), Triniti (ATCC VR-469), Una (ATCC VR-374), Venezuelan equine encephalomyelitis (ATCC VR-69, ATCC VR-923, ATCC VR-1250 ATCC VR-1249, ATCC VR-532), Western equine encephalomyelitis (ATCC VR-70, ATCC VR-1251, ATCC VR-622, ATCC VR-1252), Whataroa (ATCC VR-926), and Y-62-33 (ATCC VR-375). 
     The self-replicating RNA molecules of the invention can contain one or more modified nucleotides and therefore have improved stability and be resistant to degradation and clearance in vivo, and other advantages. Without wishing to be bound by any particular theory, it is believed that self-replicating RNA molecules that contain modified nucleotides avoid or reduce stimulation of endosomal and cytoplasmic immune receptors when the self-replicating RNA is delivered into a cell. This permits self-replication, amplification and expression of protein to occur. This also reduces safety concerns relative to self-replicating RNA that does not contain modified nucleotides, because the self-replicating RNA that contains modified nucleotides reduce activation of the innate immune system and subsequent undesired consequences (e.g., inflammation at injection site, irritation at injection site, pain, and the like). It is also believed that the RNA molecules produced as a result of self-replication are recognized as foreign nucleic acids by the cytoplasmic immune receptors. Thus, self-replicating RNA molecules that contain modified nucleotides provide for efficient amplification of the RNA in a host cell and expression of CMV proteins, as well as adjuvant effects. 
     The RNA sequence can be modified with respect to its codon usage, for example, to increase translation efficacy and half-life of the RNA. A poly A tail (e.g., of about 30 adenosine residues or more) may be attached to the 3′ end of the RNA to increase its half-life. The 5′ end of the RNA may be capped with a modified ribonucleotide with the structure m7G (5′) ppp (5′) N (cap 0 structure) or a derivative thereof, which can be incorporated during RNA synthesis or can be enzymatically engineered after RNA transcription (e.g., by using Vaccinia Virus Capping Enzyme (VCE) consisting of mRNA triphosphatase, guanylyl-transferase and guanine-7-methytransferase, which catalyzes the construction of N7-monomethylated cap 0 structures). Cap 0 structure can provide stability and translational efficacy to the RNA molecule. The 5′ cap of the RNA molecule may be further modified by a 2′-O-Methyltransferase which results in the generation of a cap 1 structure (m7Gppp [m2′-O] N), which may further increases translation efficacy. A cap 1 structure may also increase in vivo potency. 
     As used herein, “modified nucleotide” refers to a nucleotide that contains one or more chemical modifications (e.g., substitutions) in or on the nitrogenous base of the nucleoside (e.g., cytosine (C), thymine (T) or uracil (U), adenine (A) or guanine (G)). If desired, a self replicating RNA molecule can contain chemical modifications in or on the sugar moiety of the nucleoside (e.g., ribose, deoxyribose, modified ribose, modified deoxyribose, six-membered sugar analog, or open-chain sugar analog), or the phosphate. 
     The self-replicating RNA molecules can contain at least one modified nucleotide, that preferably is not part of the 5′ cap (e.g., in addition to the modification that are part of the 5″ cap). Accordingly, the self-replicating RNA molecule can contain a modified nucleotide at a single position, can contain a particular modified nucleotide (e.g., pseudouridine, N6-methyladenosine, 5-methylcytidine, 5-methyluridine) at two or more positions, or can contain two, three, four, five, six, seven, eight, nine, ten or more modified nucleotides (e.g., each at one or more positions). Preferably, the self-replicating RNA molecules comprise modified nucleotides that contain a modification on or in the nitrogenous base, but do not contain modified sugar or phosphate moieties. 
     In some examples, between 0.001% and 99% or 100% of the nucleotides in a self-replicating RNA molecule are modified nucleotides. For example, 0.001%-25%, 0.01%-25%, 0.1%-25%, or 1%-25% of the nucleotides in a self-replicating RNA molecule are modified nucleotides. 
     In other examples, between 0.001% and 99% or 100% of a particular unmodified nucleotide in a self-replicating RNA molecule is replaced with a modified nucleotide. For example, about 1% of the nucleotides in the self-replicating RNA molecule that contain uridine can be modified, such as by replacement of uridine with pseudouridine. In other examples, the desired amount (percentage) of two, three, or four particular nucleotides (nucleotides that contain uridine, cytidine, guanosine, or adenine) in a self-replicating RNA molecule are modified nucleotides. For example, 0.001%-25%, 0.01%-25%, 0.1%-25, or 1%-25% of a particular nucleotide in a self-replicating RNA molecule are modified nucleotides. In other examples, 0.001%-20%, 0.001%-15%, 0.001%-10%, 0.01%-20%, 0.01%-15%, 0.1%-25, 0.01%-10%, 1%-20%, 1%-15%, 1%-10%, or about 5%, about 10%, about 15%, about 20% of a particular nucleotide in a self-replicating RNA molecule are modified nucleotides. 
     It is preferred that less than 100% of the nucleotides in a self-replicating RNA molecule are modified nucleotides. It is also preferred that less than 100% of a particular nucleotide in a self-replicating RNA molecule are modified nucleotides. Thus, preferred self-replicating RNA molecules comprise at least some unmodified nucleotides. 
     There are more than 96 naturally occurring nucleoside modifications found on mammalian RNA. See, e.g., Limbach et al.,  Nucleic Acids Research,  22(12):2183-2196 (1994). The preparation of nucleotides and modified nucleotides and nucleosides are well-known in the art, e.g. from U.S. Pat. Nos. 4,373,071, 4,458,066, 4,500,707, 4,668,777, 4,973,679, 5,047,524, 5,132,418, 5,153,319, 5,262,530, 5,700,642 all of which are incorporated herein by reference in their entirety, and many modified nucleosides and modified nucleotides are commercially available. 
     Modified nucleobases which can be incorporated into modified nucleosides and nucleotides and be present in the RNA molecules include: m5C (5-methylcytidine), m5U (5-methyluridine), m6A (N6-methyladenosine), s2U (2-thiouridine), Um (2′-O-methyluridine), m1A (1-methyladenosine); m2A (2-methyladenosine); Am (2-1-O-methyladenosine); ms2m6A (2-methylthio-N6-methyladenosine); i6A (N6-isopentenyladenosine); ms2i6A (2-methylthio-N6isopentenyladenosine); io6A (N6-(cis-hydroxyisopentenyl)adenosine); ms2io6A (2-methylthio-N6-(cis-hydroxyisopentenyl) adenosine); g6A (N6-glycinylcarbamoyladenosine); t6A (N6-threonyl carbamoyladenosine); ms2t6A (2-methylthio-N6-threonyl carbamoyladenosine); m6t6A (N6-methyl-N6-threonylcarbamoyladenosine); hn6A (N6-hydroxynorvalylcarbamoyl adenosine); ms2hn6A (2-methylthio-N6-hydroxynorvalyl carbamoyladenosine); Ar(p) (2′-O-ribosyladenosine (phosphate)); I (inosine); m1I (1-methylinosine); m′Im (1,2′-O-dimethylinosine); m3C (3-methylcytidine); Cm (2T-O-methylcytidine); s2C (2-thiocytidine); ac4C (N4-acetylcytidine); f5C (5-fonnylcytidine); m5Cm (5,2-O-dimethylcytidine); ac4Cm (N4acetyl2TOmethylcytidine); 1(2C (lysidine); m1G (1-methylguanosine); m2G (N2-methylguanosine); m7G (7-methylguanosine); Gm (2′-O-methylguanosine); m22G (N2,N2-dimethylguanosine); m2Gm (N2,2′-O-dimethylguanosine); m22Gm (N2,N2,2′-O-trimethylguanosine); Gr(p) (2′-O-ribosylguanosine (phosphate)); yW (wybutosine); o2yW (peroxywybutosine); OHyW (hydroxywybutosine); OHyW* (undermodified hydroxywybutosine); imG (wyosine); mimG (methylguanosine); Q (queuosine); oQ (epoxyqueuosine); galQ (galtactosyl-queuosine); manQ (mannosyl-queuosine); preQo (7-cyano-7-deazaguanosine); preQi (7-aminomethyl-7-deazaguanosine); G* (archaeosine); D (dihydrouridine); m5Um (5,2′-O-dimethyluridine); s4U (4-thiouridine); m5s2U (5-methyl-2-thiouridine); s2Um (2-thio-2′-O-methyluridine); acp3U (3-(3-amino-3-carboxypropyl)uridine); ho5U (5-hydroxyuridine); mo5U (5-methoxyuridine); cmo5U (uridine 5-oxyacetic acid); mcmo5U (uridine 5-oxyacetic acid methyl ester); chm5U (5-(carboxyhydroxymethyl)uridine)); mchm5U (5-(carboxyhydroxymethyl)uridine methyl ester); mcm5U (5-methoxycarbonyl methyluridine); mcm5Um (S-methoxycarbonylmethyl-2-O-methyluridine); mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine); nm5s2U (5-aminomethyl-2-thiouridine); mnm5U (5-methylaminomethyluridine); mnm5s2U (5-methylaminomethyl-2-thiouridine); mnm5se2U (5-methylaminomethyl-2-selenouridine); ncm5U (5-carbamoylmethyl uridine); ncm5Um (5-carbamoylmethyl-2′-O-methyluridine); cmnm5U (5-carboxymethylaminomethyluridine); cnmm5Um (5-carboxymethylaminomethyl-2-L-Omethyluridine); cmnm5s2U (5-carboxymethylaminomethyl-2-thiouridine); m62A (N6,N6-dimethyladenosine); Tm (2′-O-methylinosine); m4C (N4-methylcytidine); m4Cm (N4,2-O-dimethylcytidine); hm5C (5-hydroxymethylcytidine); m3U (3-methyluridine); cm5U (5-carboxymethyluridine); m6Am (N6,T-O-dimethyladenosine); rn62Am (N6,N6,O-2-trimethyladenosine); m2′7G (N2,7-dimethylguanosine); m2′2′7G (N2,N2,7-trimethylguanosine); m3Um (3,2T-O-dimethyluridine); m5D (5-methyldihydrouridine); f5Cm (5-formyl-2′-O-methylcytidine); m1Gm (1,2′-O-dimethylguanosine); m′Am (1,2-O-dimethyl adenosine) irinomethyluridine); tm5s2U (S-taurinomethyl-2-thiouridine)); imG-14 (4-demethyl guanosine); imG2 (isoguanosine); ac6A (N6-acetyladenosine), hypoxanthine, inosine, 8-oxo-adenine, 7-substituted derivatives thereof, dihydrouracil, pseudouracil, 2-thiouracil, 4-thiouracil, 5-aminouracil, 5-(C 1 -C 6 )-alkyluracil, 5-methyluracil, 5-(C 2 -C 6 )-alkenyluracil, 5-(C 2 -C 6 )-alkynyluracil, 5-(hydroxymethyl)uracil, 5-chlorouracil, 5-fluorouracil, 5-bromouracil, 5-hydroxycytosine, 5-(C 1 -C 6 )-alkylcytosine, 5-methylcytosine, 5-(C 2 -C 6 )-alkenylcytosine, 5-(C 2 -C 6 )-alkynylcytosine, 5-chlorocytosine, 5-fluorocytosine, 5-bromocytosine, N 2 -dimethylguanine, 7-deazaguanine, 8-azaguanine, 7-deaza-7-substituted guanine, 7-deaza-7-(C 2 -C 6 )alkynylguanine, 7-deaza-8-substituted guanine, 8-hydroxyguanine, 6-thioguanine, 8-oxoguanine, 2-aminopurine, 2-amino-6-chloropurine, 2,4-diaminopurine, 2,6-diaminopurine, 8-azapurine, substituted 7-deazapurine, 7-deaza-7-substituted purine, 7-deaza-8-substituted purine, hydrogen (abasic residue), m5C, m5U, m6A, s2U, W, or 2′-O-methyl-U. Any one or any combination of these modified nucleobases may be included in the self-replicating RNA of the invention. Many of these modified nucleobases and their corresponding ribonucleosides are available from commercial suppliers. 
     If desired, the self-replicating RNA molecule can contain phosphoramidate, phosphorothioate, and/or methylphosphonate linkages. 
     Self-replicating RNA molecules that comprise at least one modified nucleotide can be prepared using any suitable method. Several suitable methods are known in the art for producing RNA molecules that contain modified nucleotides. For example, a self-replicating RNA molecule that contains modified nucleotides can be prepared by transcribing (e.g., in vitro transcription) a DNA that encodes the self-replicating RNA molecule using a suitable DNA-dependent RNA polymerase, such as T7 phage RNA polymerase, SP6 phage RNA polymerase, T3 phage RNA polymerase, and the like, or mutants of these polymerases which allow efficient incorporation of modified nucleotides into RNA molecules. The transcription reaction will contain nucleotides and modified nucleotides, and other components that support the activity of the selected polymerase, such as a suitable buffer, and suitable salts. The incorporation of nucleotide analogs into a self-replicating RNA may be engineered, for example, to alter the stability of such RNA molecules, to increase resistance against RNases, to establish replication after introduction into appropriate host cells (“infectivity” of the RNA), and/or to induce or reduce innate and adaptive immune responses. 
     Suitable synthetic methods can be used alone, or in combination with one or more other methods (e.g., recombinant DNA or RNA technology), to produce a self-replicating RNA molecule that contain one or more modified nucleotides. Suitable methods for de novo synthesis are well-known in the art and can be adapted for particular applications. Exemplary methods include, for example, chemical synthesis using suitable protecting groups such as CEM (Masuda et al., (2007)  Nucleic Acids Symposium Series  51:3-4), the β-cyanoethyl phosphoramidite method (Beaucage S L et al. (1981)  Tetrahedron Lett  22:1859); nucleoside H-phosphonate method (Garegg P et al. (1986)  Tetrahedron Lett  27:4051-4; Froehler B C et al. (1986)  Nucl Acid Res  14:5399-407; Garegg P et al. (1986)  Tetrahedron Lett  27:4055-8; Gaffney B L et al. (1988)  Tetrahedron Lett  29:2619-22). These chemistries can be performed or adapted for use with automated nucleic acid synthesizers that are commercially available. Additional suitable synthetic methods are disclosed in Uhlmann et al. (1990)  Chem Rev  90:544-84, and Goodchild J (1990)  Bioconjugate Chem  1: 165. Nucleic acid synthesis can also be performed using suitable recombinant methods that are well-known and conventional in the art, including cloning, processing, and/or expression of polynucleotides and gene products encoded by such polynucleotides. DNA shuffling by random fragmentation and PCR reassembly of gene fragments and synthetic polynucleotides are examples of known techniques that can be used to design and engineer polynucleotide sequences. Site-directed mutagenesis can be used to alter nucleic acids and the encoded proteins, for example, to insert new restriction sites, alter glycosylation patterns, change codon preference, produce splice variants, introduce mutations and the like. Suitable methods for transcription, translation and expression of nucleic acid sequences are known and conventional in the art. (See generally, Current Protocols in Molecular Biology, Vol. 2, Ed. Ausubel, et al., Greene Publish. Assoc. &amp; Wiley Interscience, Ch. 13, 1988; Glover, DNA Cloning, Vol. II, IRL Press, Wash., D.C., Ch. 3, 1986; Bitter, et al., in Methods in Enzymology 153:516-544 (1987); The Molecular Biology of the Yeast  Saccharomyces , Eds. Strathern et al., Cold Spring Harbor Press, Vols. I and II, 1982; and Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, 1989.) 
     The presence and/or quantity of one or more modified nucleotides in a self-replicating RNA molecule can be determined using any suitable method. For example, a self-replicating RNA can be digested to monophosphates (e.g., using nuclease P1) and dephosphorylated (e.g., using a suitable phosphatase such as CIAP), and the resulting nucleosides analyzed by reversed phase HPLC (e.g., usings a YMC Pack ODS-AQ column (5 micron, 4.6×250 mm) and elute using a gradient, 30% B (0-5 min) to 100% B (5-13 min) and at 100% B (13-40) min, flow Rate (0.7 ml/min), UV detection (wavelength: 260 nm), column temperature (30° C.). Buffer A (20 mM acetic acid-ammonium acetate pH 3.5), buffer B (20 mM acetic acid-ammonium acetate pH 3.5/methanol [90/10])). 
     The self-replicating RNA may be associated with a delivery system. The self-replicating RNA may be administered with or without an adjuvant. 
     RNA Delivery Systems 
     The self-replicating RNA described herein are suitable for delivery in a variety of modalities, such as naked RNA delivery or in combination with lipids, polymers or other compounds that facilitate entry into the cells. Self-replicating RNA molecules can be introduced into target cells or subjects using any suitable technique, e.g., by direct injection, microinjection, electroporation, lipofection, biolystics, and the like. The self-replicating RNA molecule may also be introduced into cells by way of receptor-mediated endocytosis. See e.g., U.S. Pat. No. 6,090,619; Wu and Wu, J. Biol. Chem., 263:14621 (1988); and Curiel et al., Proc. Natl. Acad. Sci. USA, 88:8850 (1991). For example, U.S. Pat. No. 6,083,741 discloses introducing an exogenous nucleic acid into mammalian cells by associating the nucleic acid to a polycation moiety (e.g., poly-L-lysine having 3-100 lysine residues (SEQ ID NO:4)), which is itself coupled to an integrin receptor-binding moiety (e.g., a cyclic peptide having the sequence Arg-Gly-Asp (SEQ ID NO:5). 
     The self-replicating RNA molecules can be delivered into cells via amphiphiles. See e.g., U.S. Pat. No. 6,071,890. Typically, a nucleic acid molecule may form a complex with the cationic amphiphile. Mammalian cells contacted with the complex can readily take it up. 
     The self-replicating RNA can be delivered as naked RNA (e.g. merely as an aqueous solution of RNA) but, to enhance entry into cells and also subsequent intercellular effects, the self-replicating RNA is preferably administered in combination with a delivery system, such as a particulate or emulsion delivery system. A large number of delivery systems are well known to those of skill in the art. Such delivery systems include, for example liposome-based delivery (Debs and Zhu (1993) WO 93/24640; Mannino and Gould-Fogerite (1988) BioTechniques 6(7): 682-691; Rose U.S. Pat. No. 5,279,833; Brigham (1991) WO 91/06309; and Felgner et al. (1987) Proc. Natl. Acad. Sci. USA 84: 7413-7414), as well as use of viral vectors (e.g., adenoviral (see, e.g., Berns et al. (1995) Ann. NY Acad. Sci. 772: 95-104; Ali et al. (1994) Gene Ther. 1: 367-384; and Haddada et al. (1995) Curr. Top. Microbiol. Immunol. 199 (Pt 3): 297-306 for review), papillomaviral, retroviral (see, e.g., Buchscher et al. (1992) J. Virol. 66(5) 2731-2739; Johann et al. (1992) J. Virol. 66 (5): 1635-1640 (1992); Sommerfelt et al., (1990) Virol. 176:58-59; Wilson et al. (1989) J. Virol. 63:2374-2378; Miller et al., J. Virol. 65:2220-2224 (1991); Wong-Staal et al., PCT/US94/05700, and Rosenburg and Fauci (1993) in Fundamental Immunology, Third Edition Paul (ed) Raven Press, Ltd., New York and the references therein, and Yu et al., Gene Therapy (1994) supra.), and adeno-associated viral vectors (see, West et al. (1987) Virology 160:38-47; Carter et al. (1989) U.S. Pat. No. 4,797,368; Carter et al. WO 93/24641 (1993); Kotin (1994) Human Gene Therapy 5:793-801; Muzyczka (1994) J. Clin. Invst. 94:1351 and Samulski (supra) for an overview of AAV vectors; see also, Lebkowski, U.S. Pat. No. 5,173,414; Tratschin et al. (1985) Mol. Cell. Biol. 5(11):3251-3260; Tratschin, et al. (1984) Mol. Cell. Biol., 4:2072-2081; Hermonat and Muzyczka (1984) Proc. Natl. Acad. Sci. USA, 81:6466-6470; McLaughlin et al. (1988) and Samulski et al. (1989) J. Virol., 63:03822-3828), and the like. 
     Three particularly useful delivery systems are (i) liposomes, (ii) non-toxic and biodegradable polymer microparticles, and (iii) cationic submicron oil-in-water emulsions. 
     Liposomes 
     Various amphiphilic lipids can form bilayers in an aqueous environment to encapsulate a RNA-containing aqueous core as a liposome. These lipids can have an anionic, cationic or zwitterionic hydrophilic head group. Formation of liposomes from anionic phospholipids dates back to the 1960s, and cationic liposome-forming lipids have been studied since the 1990s. Some phospholipids are anionic whereas other are zwitterionic. Suitable classes of phospholipid include, but are not limited to, phosphatidylethanolamines, phosphatidylcholines, phosphatidylserines, and phosphatidylglycerols, and some useful phospholipids are listed in Table 2. Useful cationic lipids include, but are not limited to, dioleoyl trimethylammonium propane (DOTAP), 1,2-distearyloxy-N,N-dimethyl-3-aminopropane (DSDMA), 1,2-dioleyloxy-N,Ndimethyl-3-aminopropane (DODMA), 1,2-dilinoleyloxy-N,N-dimethyl-3-aminopropane (DLinDMA), 1,2-dilinolenyloxy-N,N-dimethyl-3-aminopropane (DLenDMA). Zwitterionic lipids include, but are not limited to, acyl zwitterionic lipids and ether zwitterionic lipids. Examples of useful zwitterionic lipids are DPPC, DOPC and dodecylphosphocholine. The lipids can be saturated or unsaturated. 
     Liposomes can be formed from a single lipid or from a mixture of lipids. A mixture may comprise (i) a mixture of anionic lipids (ii) a mixture of cationic lipids (iii) a mixture of zwitterionic lipids (iv) a mixture of anionic lipids and cationic lipids (v) a mixture of anionic lipids and zwitterionic lipids (vi) a mixture of zwitterionic lipids and cationic lipids or (vii) a mixture of anionic lipids, cationic lipids and zwitterionic lipids. Similarly, a mixture may comprise both saturated and unsaturated lipids. For example, a mixture may comprise DSPC (zwitterionic, saturated), DlinDMA (cationic, unsaturated), and/or DMPG (anionic, saturated). Where a mixture of lipids is used, not all of the component lipids in the mixture need to be amphiphilic e.g. one or more amphiphilic lipids can be mixed with cholesterol. 
     The hydrophilic portion of a lipid can be PEGylated (i.e. modified by covalent attachment of a polyethylene glycol). This modification can increase stability and prevent non-specific adsorption of the liposomes. For instance, lipids can be conjugated to PEG using techniques such as those disclosed in Heyes et al. (2005)  J Controlled Release  107:276-87. 
     A mixture of DSPC, DlinDMA, PEG-DMPG and cholesterol can be used to form liposomes. A separate aspect of the invention is a liposome comprising DSPC, DlinDMA, PEG-DMG and cholesterol. This liposome preferably encapsulates RNA, such as a self-replicating RNA e.g. encoding an immunogen. 
     Liposomes are usually divided into three groups: multilamellar vesicles (MLV); small unilamellar vesicles (SUV); and large unilamellar vesicles (LUV). MLVs have multiple bilayers in each vesicle, forming several separate aqueous compartments. SUVs and LUVs have a single bilayer encapsulating an aqueous core; SUVs typically have a diameter ≤50 nm, and LUVs have a diameter &gt;50 nm. Liposomes useful with of the invention are ideally LUVs with a diameter in the range of 50-220 nm. For a composition comprising a population of LUVs with different diameters: (i) at least 80% by number should have diameters in the range of 20-220 nm, (ii) the average diameter (Zav, by intensity) of the population is ideally in the range of 40-200 nm, and/or (iii) the diameters should have a polydispersity index &lt;0.2. 
     Techniques for preparing suitable liposomes are well known in the art e.g. see Liposomes: Methods and Protocols, Volume 1: Pharmaceutical Nanocarriers: Methods and Protocols. (ed. Weissig). Humana Press, 2009. ISBN 160327359X; Liposome Technology, volumes I, II &amp; III. (ed. Gregoriadis). Informa Healthcare, 2006; and Functional Polymer Colloids and Microparticles volume 4 (Microspheres, microcapsules &amp; liposomes). (eds. Arshady &amp; Guyot). Citus Books, 2002. One useful method involves mixing (i) an ethanolic solution of the lipids (ii) an aqueous solution of the nucleic acid and (iii) buffer, followed by mixing, equilibration, dilution and purification (Heyes et al. (2005)  J Controlled Release  107:276-87.). 
     RNA is preferably encapsulated within the liposomes, and so the liposome forms a outer layer around an aqueous RNA-containing core. This encapsulation has been found to protect RNA from RNase digestion. The liposomes can include some external RNA (e.g. on the surface of the liposomes), but preferably, at least half of the RNA (and ideally substantially all of it) is encapsulated. 
     Polymeric Microparticles 
     Various polymers can form microparticles to encapsulate or adsorb RNA. The use of a substantially non-toxic polymer means that a recipient can safely receive the particles, and the use of a biodegradable polymer means that the particles can be metabolised after delivery to avoid long-term persistence. Useful polymers are also sterilisable, to assist in preparing pharmaceutical grade formulations. 
     Suitable non-toxic and biodegradable polymers include, but are not limited to, poly(α-hydroxy acids), polyhydroxy butyric acids, polylactones (including polycaprolactones), polydioxanones, polyvalerolactone, polyorthoesters, polyanhydrides, polycyanoacrylates, tyrosine-derived polycarbonates, polyvinyl-pyrrolidinones or polyester-amides, and combinations thereof. 
     In some embodiments, the microparticles are formed from poly(α-hydroxy acids), such as a poly(lactides) (“PLA”), copolymers of lactide and glycolide such as a poly(D,L-lactide-co-glycolide) (“PLG”), and copolymers of D,L-lactide and caprolactone. Useful PLG polymers include those having a lactide/glycolide molar ratio ranging, for example, from 20:80 to 80:20 e.g. 25:75, 40:60, 45:55, 55:45, 60:40, 75:25. Useful PLG polymers include those having a molecular weight between, for example, 5,000-200,000 Da e.g. between 10,000-100,000, 20,000-70,000, 40,000-50,000 Da. 
     The microparticles ideally have a diameter in the range of 0.02 μm to 8 μm. For a composition comprising a population of microparticles with different diameters at least 80% by number should have diameters in the range of 0.03-7 μm. 
     Techniques for preparing suitable microparticles are well known in the art e.g. see Functional Polymer Colloids and Microparticles volume 4 (Microspheres, microcapsules &amp; liposomes). (eds. Arshady &amp; Guyot). Citus Books, 2002 ; Polymers in Drug Delivery . (eds. Uchegbu &amp; Schatzlein). CRC Press, 2006. (in particular chapter 7) and  Microparticulate Systems for the Delivery of Proteins and Vaccines . (eds. Cohen &amp; Bernstein). CRC Press, 1996. To facilitate adsorption of RNA, a microparticle may include a cationic surfactant and/or lipid e.g. as disclosed in O&#39;Hagan et al. (2001)  J Virology  75:9037-9043; and Singh et al. (2003)  Pharmaceutical Research  20: 247-251. An alternative way of making polymeric microparticles is by molding and curing e.g. as disclosed in WO2009/132206. 
     Microparticles of the invention can have a zeta potential of between 40-100 mV. RNA can be adsorbed to the microparticles, and adsorption is facilitated by including cationic materials (e.g. cationic lipids) in the microparticle. 
     Oil-In-Water Cationic Emulsions 
     Oil-in-water emulsions are known for adjuvanting influenza vaccines e.g. the MF59™ adjuvant in the FLUAD™ product, and the AS03 adjuvant in the PREPANDRIX™ product. RNA delivery can be accomplished with the use of an oil-in-water emulsion, provided that the emulsion includes one or more cationic molecules. For instance, a cationic lipid can be included in the emulsion to provide a positively charged droplet surface to which negatively-charged RNA can attach. 
     The emulsion comprises one or more oils. Suitable oil(s) include those from, for example, an animal (such as fish) or a vegetable source. The oil is ideally biodegradable (metabolizable) and biocompatible. Sources for vegetable oils include nuts, seeds and grains. Peanut oil, soybean oil, coconut oil, and olive oil, the most commonly available, exemplify the nut oils. Jojoba oil can be used e.g. obtained from the jojoba bean. Seed oils include safflower oil, cottonseed oil, sunflower seed oil, sesame seed oil and the like. In the grain group, corn oil is the most readily available, but the oil of other cereal grains such as wheat, oats, rye, rice, teff, triticale and the like may also be used. 6-10 carbon fatty acid esters of glycerol and 1,2-propanediol, while not occurring naturally in seed oils, may be prepared by hydrolysis, separation and esterification of the appropriate materials starting from the nut and seed oils. Fats and oils from mammalian milk are metabolizable and so may be used. The procedures for separation, purification, saponification and other means necessary for obtaining pure oils from animal sources are well known in the art. 
     Most fish contain metabolizable oils which may be readily recovered. For example, cod liver oil, shark liver oils, and whale oil such as spermaceti exemplify several of the fish oils which may be used herein. A number of branched chain oils are synthesized biochemically in 5-carbon isoprene units and are generally referred to as terpenoids. Squalane, the saturated analog to squalene, can also be used. Fish oils, including squalene and squalane, are readily available from commercial sources or may be obtained by methods known in the art. 
     Other useful oils are the tocopherols, particularly in combination with squalene. Where the oil phase of an emulsion includes a tocopherol, any of the α, β, γ, δ, ϵ or ξ tocopherols can be used, but α-tocopherols are preferred. D-α-tocopherol and DL-α-tocopherol can both be used. A preferred α-tocopherol is DL-α-tocopherol. An oil combination comprising squalene and a tocopherol (e.g. DL-α-tocopherol) can be used. 
     Preferred emulsions comprise squalene, a shark liver oil which is a branched, unsaturated terpenoid (C 30 H 50 ; [(CH 3 ) 2 C[═CHCH 2 CH 2 C(CH 3 )] 2 ═CHCH 2 —] 2 ; 2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene; CAS RN 7683-64-9). 
     The oil in the emulsion may comprise a combination of oils e.g. squalene and at least one further oil. 
     The aqueous component of the emulsion can be plain water (e.g. w.f.i.) or can include further components e.g. solutes. For instance, it may include salts to form a buffer e.g. citrate or phosphate salts, such as sodium salts. Typical buffers include: a phosphate buffer; a Tris buffer; a borate buffer; a succinate buffer; a histidine buffer; or a citrate buffer. A buffered aqueous phase is preferred, and buffers will typically be included in the 5-20 mM range. 
     The emulsion also includes a cationic lipid. Preferably this lipid is a surfactant so that it can facilitate formation and stabilization of the emulsion. Useful cationic lipids generally contains a nitrogen atom that is positively charged under physiological conditions e.g. as a tertiary or quaternary amine. This nitrogen can be in the hydrophilic head group of an amphiphilic surfactant. Useful cationic lipids include, but are not limited to: 1,2-dioleoyloxy-3-(trimethylammonio)propane (DOTAP), 3′-[N-(N′,N′-Dimethylaminoethane)-carbamoyl]Cholesterol (DC Cholesterol), dimethyldioctadecyl-ammonium (DDA e.g. the bromide), 1,2-Dimyristoyl-3-Trimethyl-AmmoniumPropane (DMTAP), dipalmitoyl(C16:0)trimethyl ammonium propane (DPTAP), distearoyltrimethylammonium propane (DSTAP). Other useful cationic lipids are: benzalkonium chloride (BAK), benzethonium chloride, cetramide (which contains tetradecyltrimethylammonium bromide and possibly small amounts of dedecyltrimethylammonium bromide and hexadecyltrimethyl ammonium bromide), cetylpyridinium chloride (CPC), cetyl trimethylammonium chloride (CTAC), N,N′,N′-polyoxyethylene (10)-N-tallow-1,3-diaminopropane, dodecyltrimethylammonium bromide, hexadecyltrimethyl-ammonium bromide, mixed alkyl-trimethyl-ammonium bromide, benzyldimethyldodecylammonium chloride, benzyldimethylhexadecyl-ammonium chloride, benzyltrimethylammonium methoxide, cetyldimethylethylammonium bromide, dimethyldioctadecyl ammonium bromide (DDAB), methylbenzethonium chloride, decamethonium chloride, methyl mixed trialkyl ammonium chloride, methyl trioctylammonium chloride), N,N-dimethyl-N-[2 (2-methyl-4-(1,1,3,3tetramethylbutyl)-phenoxy]-ethoxy)ethyl]-benzenemetha-naminium chloride (DEBDA), dialkyldimetylammonium salts, [1-(2,3-dioleyloxy)-propyl]-N,N,N,trimethylammonium chloride, 1,2-diacyl-3-(trimethylammonio) propane (acyl group=dimyristoyl, dipalmitoyl, distearoyl, dioleoyl), 1,2-diacyl-3 (dimethylammonio)propane (acyl group=dimyristoyl, dipalmitoyl, distearoyl, dioleoyl), 1,2-dioleoyl-3-(4′-trimethyl-ammonio)butanoyl-sn-glycerol, 1,2-dioleoyl 3-succinyl-sn-glycerol choline ester, cholesteryl (4′-trimethylammonio) butanoate), N-alkyl pyridinium salts (e.g. cetylpyridinium bromide and cetylpyridinium chloride), N-alkylpiperidinium salts, dicationic bolaform electrolytes (C12Me6; C12BU6), dialkylglycetylphosphorylcholine, lysolecithin, L-α dioleoylphosphatidylethanolamine, cholesterol hemisuccinate choline ester, lipopolyamines, including but not limited to dioctadecylamidoglycylspermine (DOGS), dipalmitoyl phosphatidylethanol-amidospermine (DPPES), lipopoly-L (or D)-lysine (LPLL, LPDL), poly (L (or D)-lysine conjugated to N-glutarylphosphatidylethanolamine, didodecyl glutamate ester with pendant amino group (C{circumflex over ( )}GluPhCnN), ditetradecyl glutamate ester with pendant amino group (C14GIuCnN+), cationic derivatives of cholesterol, including but not limited to cholesteryl-3 β-oxysuccinamidoethylenetrimethylammonium salt, cholesteryl-3 β-oxysuccinamidoethylene-dimethylamine, cholesteryl-3 β-carboxyamidoethylenetrimethylammonium salt, and cholesteryl-3 β-carboxyamidoethylenedimethylamine Other useful cationic lipids are described in US 2008/0085870 and US 2008/0057080, which are incorporated herein by reference. The cationic lipid is preferably biodegradable (metabolizable) and biocompatible. 
     In addition to the oil and cationic lipid, an emulsion can include a non-ionic surfactant and/or a zwitterionic surfactant. Such surfactants include, but are not limited to: the polyoxyethylene sorbitan esters surfactants (commonly referred to as the Tweens), especially polysorbate 20 and polysorbate 80; copolymers of ethylene oxide (EO), propylene oxide (PO), and/or butylene oxide (BO), sold under the DOWFAX™ tradename, such as linear EO/PO block copolymers; octoxynols, which can vary in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups, with octoxynol-9 (Triton X-100, or t-octylphenoxypolyethoxyethanol) being of particular interest; (octylphenoxy)polyethoxyethanol (IGEPAL CA-630/NP-40); phospholipids such as phosphatidylcholine (lecithin); polyoxyethylene fatty ethers derived from lauryl, cetyl, stearyl and oleyl alcohols (known as Brij surfactants), such as triethyleneglycol monolauryl ether (Brij 30); polyoxyethylene-9-lauryl ether; and sorbitan esters (commonly known as the Spans), such as sorbitan trioleate (Span 85) and sorbitan monolaurate. Preferred surfactants for including in the emulsion are polysorbate 80 (Tween 80; polyoxyethylene sorbitan monooleate), Span 85 (sorbitan trioleate), lecithin and Triton X-100. 
     Mixtures of these surfactants can be included in the emulsion e.g. Tween 80/Span 85 mixtures, or Tween 80/Triton-X100 mixtures. A combination of a polyoxyethylene sorbitan ester such as polyoxyethylene sorbitan monooleate (Tween 80) and an octoxynol such as t-octylphenoxy-polyethoxyethanol (Triton X-100) is also suitable. Another useful combination comprises laureth 9 plus a polyoxyethylene sorbitan ester and/or an octoxynol. Useful mixtures can comprise a surfactant with a HLB value in the range of 10-20 (e.g. polysorbate 80, with a HLB of 15.0) and a surfactant with a HLB value in the range of 1-10 (e.g. sorbitan trioleate, with a HLB of 1.8). 
     Preferred amounts of oil (% by volume) in the final emulsion are between 2-20% e.g. 5-15%, 6-14%, 7-13%, 8-12%. A squalene content of about 4-6% or about 9-11% is particularly useful. 
     Preferred amounts of surfactants (% by weight) in the final emulsion are between 0.001% and 8%. For example: polyoxyethylene sorbitan esters (such as polysorbate 80) 0.2 to 4%, in particular between 0.4-0.6%, between 0.45-0.55%, about 0.5% or between 1.5-2%, between 1.8-2.2%, between 1.9-2.1%, about 2%, or 0.85-0.95%, or about 1%; sorbitan esters (such as sorbitan trioleate) 0.02 to 2%, in particular about 0.5% or about 1%; octyl- or nonylphenoxy polyoxyethanols (such as Triton X-100) 0.001 to 0.1%, in particular 0.005 to 0.02%; polyoxyethylene ethers (such as laureth 9) 0.1 to 8%, preferably 0.1 to 10% and in particular 0.1 to 1% or about 0.5%. 
     The absolute amounts of oil and surfactant, and their ratio, can be varied within wide limits while still forming an emulsion. A skilled person can easily vary the relative proportions of the components to obtain a desired emulsion, but a weight ratio of between 4:1 and 5:1 for oil and surfactant is typical (excess oil). 
     An important parameter for ensuring immunostimulatory activity of an emulsion, particularly in large animals, is the oil droplet size (diameter). The most effective emulsions have a droplet size in the submicron range. Suitably the droplet sizes will be in the range 50-750 nm. Most usefully the average droplet size is less than 250 nm e.g. less than 200 nm, less than 150 nm. The average droplet size is usefully in the range of 80-180 nm. Ideally, at least 80% (by number) of the emulsion&#39;s oil droplets are less than 250 nm in diameter, and preferably at least 90%. Apparatuses for determining the average droplet size in an emulsion, and the size distribution, are commercially available. These typically use the techniques of dynamic light scattering and/or single-particle optical sensing e.g. the Accusizer™ and Nicomp™ series of instruments available from Particle Sizing Systems (Santa Barbara, USA), or the Zetasizer™ instruments from Malvern Instruments (UK), or the Particle Size Distribution Analyzer instruments from Horiba (Kyoto, Japan). 
     Ideally, the distribution of droplet sizes (by number) has only one maximum i.e. there is a single population of droplets distributed around an average (mode), rather than having two maxima. Preferred emulsions have a polydispersity of &lt;0.4 e.g. 0.3, 0.2, or less. 
     Suitable emulsions with submicron droplets and a narrow size distribution can be obtained by the use of microfluidization. This technique reduces average oil droplet size by propelling streams of input components through geometrically fixed channels at high pressure and high velocity. These streams contact channel walls, chamber walls and each other. The results shear, impact and cavitation forces cause a reduction in droplet size. Repeated steps of microfluidization can be performed until an emulsion with a desired droplet size average and distribution are achieved. 
     As an alternative to microfluidization, thermal methods can be used to cause phase inversion. These methods can also provide a submicron emulsion with a tight particle size distribution. 
     Preferred emulsions can be filter sterilized i.e. their droplets can pass through a 220 nm filter. As well as providing a sterilization, this procedure also removes any large droplets in the emulsion. 
     In certain embodiments, the cationic lipid in the emulsion is DOTAP. The cationic oil-in-water emulsion may comprise from about 0.5 mg/ml to about 25 mg/ml DOTAP. For example, the cationic oil-in-water emulsion may comprise DOTAP at from about 0.5 mg/ml to about 25 mg/ml, from about 0.6 mg/ml to about 25 mg/ml, from about 0.7 mg/ml to about 25 mg/ml, from about 0.8 mg/ml to about 25 mg/ml, from about 0.9 mg/ml to about 25 mg/ml, from about 1.0 mg/ml to about 25 mg/ml, from about 1.1 mg/ml to about 25 mg/ml, from about 1.2 mg/ml to about 25 mg/ml, from about 1.3 mg/ml to about 25 mg/ml, from about 1.4 mg/ml to about 25 mg/ml, from about 1.5 mg/ml to about 25 mg/ml, from about 1.6 mg/ml to about 25 mg/ml, from about 1.7 mg/ml to about 25 mg/ml, from about 0.5 mg/ml to about 24 mg/ml, from about 0.5 mg/ml to about 22 mg/ml, from about 0.5 mg/ml to about 20 mg/ml, from about 0.5 mg/ml to about 18 mg/ml, from about 0.5 mg/ml to about 15 mg/ml, from about 0.5 mg/ml to about 12 mg/ml, from about 0.5 mg/ml to about 10 mg/ml, from about 0.5 mg/ml to about 5 mg/ml, from about 0.5 mg/ml to about 2 mg/ml, from about 0.5 mg/ml to about 1.9 mg/ml, from about 0.5 mg/ml to about 1.8 mg/ml, from about 0.5 mg/ml to about 1.7 mg/ml, from about 0.5 mg/ml to about 1.6 mg/ml, from about 0.6 mg/ml to about 1.6 mg/ml, from about 0.7 mg/ml to about 1.6 mg/ml, from about 0.8 mg/ml to about 1.6 mg/ml, about 0.5 mg/ml, about 0.6 mg/ml, about 0.7 mg/ml, about 0.8 mg/ml, about 0.9 mg/ml, about 1.0 mg/ml, about 1.1 mg/ml, about 1.2 mg/ml, about 1.3 mg/ml, about 1.4 mg/ml, about 1.5 mg/ml, about 1.6 mg/ml, about 12 mg/ml, about 18 mg/ml, about 20 mg/ml, about 21.8 mg/ml, about 24 mg/ml, etc. In an exemplary embodiment, the cationic oil-in-water emulsion comprises from about 0.8 mg/ml to about 1.6 mg/ml DOTAP, such as 0.8 mg/ml, 1.2 mg/ml, 1.4 mg/ml or 1.6 mg/ml. 
     In certain embodiments, the cationic lipid is DC Cholesterol. The cationic oil-in-water emulsion may comprise DC Cholesterol at from about 0.1 mg/ml to about 5 mg/ml DC Cholesterol. For example, the cationic oil-in-water emulsion may comprise DC Cholesterol from about 0.1 mg/ml to about 5 mg/ml, from about 0.2 mg/ml to about 5 mg/ml, from about 0.3 mg/ml to about 5 mg/ml, from about 0.4 mg/ml to about 5 mg/ml, from about 0.5 mg/ml to about 5 mg/ml, from about 0.62 mg/ml to about 5 mg/ml, from about 1 mg/ml to about 5 mg/ml, from about 1.5 mg/ml to about 5 mg/ml, from about 2 mg/ml to about 5 mg/ml, from about 2.46 mg/ml to about 5 mg/ml, from about 3 mg/ml to about 5 mg/ml, from about 3.5 mg/ml to about 5 mg/ml, from about 4 mg/ml to about 5 mg/ml, from about 4.5 mg/ml to about 5 mg/ml, from about 0.1 mg/ml to about 4.92 mg/ml, from about 0.1 mg/ml to about 4.5 mg/ml, from about 0.1 mg/ml to about 4 mg/ml, from about 0.1 mg/ml to about 3.5 mg/ml, from about 0.1 mg/ml to about 3 mg/ml, from about 0.1 mg/ml to about 2.46 mg/ml, from about 0.1 mg/ml to about 2 mg/ml, from about 0.1 mg/ml to about 1.5 mg/ml, from about 0.1 mg/ml to about 1 mg/ml, from about 0.1 mg/ml to about 0.62 mg/ml, about 0.15 mg/ml, about 0.3 mg/ml, about 0.6 mg/ml, about 0.62 mg/ml, about 0.9 mg/ml, about 1.2 mg/ml, about 2.46 mg/ml, about 4.92 mg/ml, etc. In an exemplary embodiment, the cationic oil-in-water emulsion comprises from about 0.62 mg/ml to about 4.92 mg/ml DC Cholesterol, such as 2.46 mg/ml. 
     In certain embodiments, the cationic lipid is DDA. The cationic oil-in-water emulsion may comprise from about 0.1 mg/ml to about 5 mg/ml DDA. For example, the cationic oil-in-water emulsion may comprise DDA at from about 0.1 mg/ml to about 5 mg/ml, from about 0.1 mg/ml to about 4.5 mg/ml, from about 0.1 mg/ml to about 4 mg/ml, from about 0.1 mg/ml to about 3.5 mg/ml, from about 0.1 mg/ml to about 3 mg/ml, from about 0.1 mg/ml to about 2.5 mg/ml, from about 0.1 mg/ml to about 2 mg/ml, from about 0.1 mg/ml to about 1.5 mg/ml, from about 0.1 mg/ml to about 1.45 mg/ml, from about 0.2 mg/ml to about 5 mg/ml, from about 0.3 mg/ml to about 5 mg/ml, from about 0.4 mg/ml to about 5 mg/ml, from about 0.5 mg/ml to about 5 mg/ml, from about 0.6 mg/ml to about 5 mg/ml, from about 0.73 mg/ml to about 5 mg/ml, from about 0.8 mg/ml to about 5 mg/ml, from about 0.9 mg/ml to about 5 mg/ml, from about 1.0 mg/ml to about 5 mg/ml, from about 1.2 mg/ml to about 5 mg/ml, from about 1.45 mg/ml to about 5 mg/ml, from about 2 mg/ml to about 5 mg/ml, from about 2.5 mg/ml to about 5 mg/ml, from about 3 mg/ml to about 5 mg/ml, from about 3.5 mg/ml to about 5 mg/ml, from about 4 mg/ml to about 5 mg/ml, from about 4.5 mg/ml to about 5 mg/ml, about 1.2 mg/ml, about 1.45 mg/ml, etc. Alternatively, the cationic oil-in-water emulsion may comprise DDA at about 20 mg/ml, about 21 mg/ml, about 21.5 mg/ml, about 21.6 mg/ml, about 25 mg/ml. In an exemplary embodiment, the cationic oil-in-water emulsion comprises from about 0.73 mg/ml to about 1.45 mg/ml DDA, such as 1.45 mg/ml. 
     Catheters or like devices may be used to deliver the self-replicating RNA molecules of the invention, as naked RNA or in combination with a delivery system, into a target organ or tissue. Suitable catheters are disclosed in, e.g., U.S. Pat. Nos. 4,186,745; 5,397,307; 5,547,472; 5,674,192; and 6,129,705, all of which are incorporated herein by reference. 
     The present invention includes the use of suitable delivery systems, such as liposomes, polymer microparticles or submicron emulsion microparticles with encapsulated or adsorbed self-replicating RNA, to deliver a self-replicating RNA molecule that encodes two or more CMV proteins, for example, to elicit an immune response alone, or in combination with another macromolecule. The invention includes liposomes, microparticles and submicron emulsions with adsorbed and/or encapsulated self-replicating RNA molecules, and combinations thereof. 
     The self-replicating RNA molecules associated with liposomes and submicron emulsion microparticles can be effectively delivered to a host cell, and can induce an immune response to the protein encoded by the self-replicating RNA. 
     Polycistronic self replicating RNA molecules that encode CMV proteins, and VRPs produced using polycistronic alphavirus replicons, can be used to form CMV protein complexes in a cell. Complexes include, but are not limited to, gB/gH/gL; gH/gL; gH/gL/gO; gM/gN; gH/gL/UL128/UL130/UL131; and UL128/UL130/UL131. 
     In some embodiments combinations of VRPs are delivered to a cell. Combinations include, but are not limited to:
         1. a gH/gL VRP and another VRP;   2. a gH/gL VRP and a gB VRP;   3. a gH/gL/gO VRP and a gB VRP;   4. a gB VRP and a gH/gL/UL128/UL130/UL131 VRP;   5. a gB VRP and UL128/UL130/UL131 VRP;   6. a gB VRP and a gM/gN VRP;   7. a gB VRP, a gH/gL VRP, and a UL128/UL130/UL131 VRP;   8. a gB VRP, a gH/gLgO VRP, and a UL128/UL130/UL131 VRP;   9. a gB VRP, a gM/gN VRP, a gH/gL VRP, and a UL128/UL130/UL131 VRP;   10. a gB VRP, a gM/gN VRP, a gH/gL/O VRP, and a UL128/UL130/UL131 VRP;   11. a gH/gL VRP and a UL128/UL130/UL131 VRP; and       

     In some embodiments combinations of self-replicating RNA molecules are delivered to a cell. Combinations include, but are not limited to:
         1. a self-replicating RNA molecule encoding gH/gL and a self-replicating RNA molecule encoding another protein;   2. a self-replicating RNA molecule encoding gH and gL and a self-replicating RNA molecule encoding gB;   3. a self-replicating RNA molecule encoding gH, gL and gO and a self-replicating RNA molecule encoding gB;   4. a self-replicating RNA molecule encoding gB and a self-replicating RNA molecule encoding gH, gL, UL128, UL130 and UL131;   5. a self-replicating RNA molecule encoding gB and a self-replicating RNA molecule encoding UL128, UL130 and UL131;   6. a self-replicating RNA molecule encoding gB and a self-replicating RNA molecule encoding gM and gN;   7. a self-replicating RNA molecule encoding gB, a self-replicating RNA molecule encoding gH and gL, and a self-replicating RNA molecule encoding UL128, UL130 and UL131;   8. a self-replicating RNA molecule encoding gB, a self-replicating RNA molecule encoding gH, gL, and gO, and a self-replicating RNA molecule encoding UL128, UL130 and UL131;   9. a self-replicating RNA molecule encoding gB, a self-replicating RNA molecule encoding gM and gN, a self-replicating RNA molecule encoding gH and gL, and a self-replicating RNA molecule encoding UL128, UL130 and UL131;   10. a self-replicating RNA molecule encoding gB, a self-replicating RNA molecule encoding gM and gN, a self-replicating RNA molecule encoding gH, gL and gO, and a self-replicating RNA molecule encoding UL128, UL130 and UL131;   11. a self-replicating RNA molecule encoding gH and gL, and a self-replicating RNA molecule encoding UL128, UL130 and UL131; and       

     CMV Proteins 
     Suitable CMV proteins include gB, gH, gL, gO, and can be from any CMV strain. Other suitable CMV proteins include UL128, UL130 and UL131, and can be from any CMV strain. For example, CMV proteins can be from Merlin, AD169, VR1814, Towne, Toledo, TR, PH, TB40, or Fix strains of CMV. Exemplary CMV proteins and fragments are described herein. These proteins and fragments can be encoded by any suitable nucleotide sequence, including sequences that are codon optimized or deoptimized for expression in a desired host, such as a human cell. Exemplary sequences of CMV proteins and nucleic acids encoding the proteins are provided in Table 2 
     
       
         
           
               
               
             
               
                 TABLE 2 
               
               
                   
               
             
            
               
                 Full length gH polynucleotide 
                 (CMV gH FL) SEQ ID NO: 31 
               
               
                 Full length gH polypeptide 
                 (CMV gH FL) SEQ ID NO: 32 
               
               
                 Full length gL polynucleotide 
                 (CMV gL FL) SEQ ID NO: 35 
               
               
                 Full length gL polypeptide 
                 (CMV gL FL) SEQ ID NO: 36 
               
               
                 Full length gO polynucleotide 
                 (CMV gO FL) SEQ ID NO: 41 
               
               
                 Full length gO polypeptide 
                 (CMV gO FL) SEQ ID NO: 42 
               
               
                 gH sol polynucleotide 
                 (CMV gH sol) SEQ ID NO: 33 
               
               
                 gH sol polypeptide 
                 (CMV gH sol) SEQ ID NO: 34 
               
               
                 Full length UL128 polynucleotide 
                 (CMV UL128 FL) SEQ ID NO: 43 
               
               
                 Full length UL128 polypeptide 
                 (CMV UL128 FL) SEQ ID NO: 44 
               
               
                 Full length UL130 polynucleotide 
                 (CMV UL130 FL) SEQ ID NO: 45 
               
               
                 Full length UL130 polypeptide 
                 (CMV UL130 FL) SEQ ID NO: 46 
               
               
                 Full length UL131 polynucleotide 
                 (CMV UL131 FL) SEQ ID NO: 47 
               
               
                 Full length UL131 polypeptide 
                 (CMV UL131 FL) SEQ ID NO: 48 
               
               
                 Full length gB polynucleotide 
                 (CMV gB FL) SEQ ID NO: 25 
               
               
                 Full length gB polypeptide 
                 (CMV gB FL) SEQ ID NO: 26 
               
               
                 gB sol 750 polynucleotide 
                 (CMV gB 750) SEQ ID NO: 27 
               
               
                 gB sol 750 polypeptide 
                 (CMV gB 750) SEQ ID NO: 28 
               
               
                 gB sol 692 polynucleotide 
                 (CMV gB 692) SEQ ID NO: 29 
               
               
                 gB sol 692 polypeptide 
                 (CMV gB 692) SEQ ID NO: 30 
               
               
                 Full length gM polynucleotide 
                 (CMV gM FL) SEQ ID NO: 37 
               
               
                 Full length gM polypeptide 
                 (CMV gM FL) SEQ ID NO: 38 
               
               
                 Full length gN polynucleotide 
                 (CMV gN FL) SEQ ID NO: 39 
               
               
                 Full length gN polypeptide 
                 (CMV gN FL) SEQ ID NO: 40 
               
               
                   
               
            
           
         
       
     
     CMV gB Proteins 
     A gB protein can be full length or can omit one or more regions of the protein. Alternatively, fragments of a gB protein can be used. gB amino acids are numbered according to the full-length gB amino acid sequence (CMV gB FL) shown in SEQ ID NO: 26, which is 907 amino acids long. Suitable regions of a gB protein, which can be excluded from the full-length protein or included as fragments include: the signal sequence (amino acids 1-24), a gB-DLD disintegrin-like domain (amino acids 57-146), a furin cleavage site (amino acids 459-460), a heptad repeat region (679-693), a membrane spanning domain (amino acids 751-771), and a cytoplasmic domain from amino acids 771-906. In some embodiments a gB protein includes amino acids 67-86 (Neutralizing Epitope AD2) and/or amino acids 532-635 (Immunodominant Epitope AD1). Specific examples of gB fragments, include “gB sol 692,” which includes the first 692 amino acids of gB, and “gB sol 750,” which includes the first 750 amino acids of gB. The signal sequence, amino acids 1-24, can be present or absent from gB sol 692 and gB sol 750 as desired. Optionally, the gB protein can be a gB fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 525, 550, 575, 600, 625, 650, 675, 700, 725, 750, 775, 800, 825, 850, or 875 amino acids. A gB fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598, 599, 600, 601, 602, 603, 604, 605, 606, 607, 608, 609, 610, 611, 612, 613, 614, 615, 616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 668, 669, 670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683, 684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729, 730, 731, 732, 733, 734, 735, 736, 737, 738, 739, 740, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767, 768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779, 780, 781, 782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794, 795, 796, 797, 798, 799, 800, 801, 802, 803, 804, 805, 806, 807, 808, 809, 810, 811, 812, 813, 814, 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828, 829, 830, 831, 832, 833, 834, 835, 836, 837, 838, 839, 840, 841, 842, 843, 844, 845, 846, 847, 848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861, 862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875, 876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889, 890, 891, 892, 893, 894, 895, 896, or 897. 
     Optionally, a gB fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a gB fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     CMV gH Proteins 
     In some embodiments, a gH protein is a full-length gH protein (CMV gH FL, SEQ ID NO: 32, for example, which is a 742 amino acid protein). gH has a membrane spanning domain and a cytoplasmic domain starting at position 716 to position 743. Removing amino acids from 717 to 743 provides a soluble gH (e.g., CMV gH sol, SEQ ID NO: 34). In some embodiments the gH protein can be a gH fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 525, 550, 575, 600, 625, 650, 675, 700, or 725 amino acids. Optionally, the gH protein can be a gH fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 525, 550, 575, 600, 625, 650, 675, 700, or 725 amino acids. A gH fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598, 599, 600, 601, 602, 603, 604, 605, 606, 607, 608, 609, 610, 611, 612, 613, 614, 615, 616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 668, 669, 670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683, 684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729, 730, 731, or 732. 
     gH residues are numbered according to the full-length gH amino acid sequence (CMV gH FL) shown in SEQ ID NO: 32. Optionally, a gH fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a gH fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     CMV gL Proteins 
     In some embodiments a gL protein is a full-length gL protein (CMV gL FL, SEQ ID NO: 36, for example, which is a 278 amino acid protein). In some embodiments a gL fragment can be used. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, or 250 amino acids. A gL fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, or 268. 
     gL residues are numbered according to the full-length gL amino acid sequence (CMV gL FL) shown in SEQ ID NO: 36. Optionally, a gL fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a gL fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     CMV gO Proteins 
     In some embodiments, a gO protein is a full-length gO protein (CMV gO FL, SEQ ID NO: 42, for example, which is a 472 amino acid protein). In some embodiments the gO protein can be a gO fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, or 450 amino acids. A gO fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, or 462. 
     gO residues are numbered according to the full-length gO amino acid sequence (CMV gO FL) shown in SEQ ID NO: 42. Optionally, a gO fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a gO fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     CMV gM Proteins 
     In some embodiments, a gM protein is a full-length gM protein (CMV gM FL, SEQ ID NO: 38, for example, which is a 371 amino acid protein). In some embodiments the gM protein can be a gM fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, or 350 amino acids. A gM fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, or 361. 
     gM residues are numbered according to the full-length gM amino acid sequence (CMV gM FL) shown in SEQ ID NO: 38. Optionally, a gM fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a gM fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     CMV gN Proteins 
     In some embodiments, a gN protein is a full-length gN protein (CMV gN FL, SEQ ID NO: 40, for example, which is a 135 amino acid protein). In some embodiments the gN protein can be a gN fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, or 125 amino acids. A gN fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, or 125. 
     gN residues are numbered according to the full-length gN amino acid sequence (CMV gN FL) shown in SEQ ID NO: 40. Optionally, a gN fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a gN fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     CMV UL128 Proteins 
     In some embodiments, a UL128 protein is a full-length UL128 protein (CMV UL128 FL, SEQ ID NO: 44, for example, which is a 171 amino acid protein). In some embodiments the UL128 protein can be a UL128 fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, or 150 amino acids. A UL128 fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, or 161. 
     UL128 residues are numbered according to the full-length UL128 amino acid sequence (CMV UL128 FL) shown in SEQ ID NO: 44. Optionally, a UL128 fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a UL128 fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     CMV UL130 Proteins 
     In some embodiments, a UL130 protein is a full-length UL130 protein (CMV UL130 FL, SEQ ID NO: 46, for example, which is a 214 amino acid protein). In some embodiments the UL130 protein can be a UL130 fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, or 200 amino acids. A UL130 fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, or 204. 
     UL130 residues are numbered according to the full-length UL130 amino acid sequence (CMV UL130 FL) shown in SEQ ID NO: 46. Optionally, a UL130 fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a UL130 fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     CMV UL131 Proteins 
     In some embodiments, a UL131 protein is a full-length UL131 protein (CMV UL131, SEQ ID NO: 48, for example, which is a 129 amino acid protein). In some embodiments the UL131 protein can be a UL131 fragment of 10 amino acids or longer. For example, the number of amino acids in the fragment can comprise 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, or 200 amino acids. A UL131 fragment can begin at any of residue number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119. 
     UL131 residues are numbered according to the full-length UL131 amino acid sequence (CMV UL131 FL) shown in SEQ ID NO: 48. Optionally, a UL131 fragment can extend further into the N-terminus by 5, 10, 20, or 30 amino acids from the starting residue of the fragment. Optionally, a UL131 fragment can extend further into the C-terminus by 5, 10, 20, or 30 amino acids from the last residue of the fragment. 
     As stated above, the invention relates to recombinant polycistronic nucleic acid molecules that contain a first sequence encoding a first herpesvirus protein or fragment thereof, and a second sequence encoding a second herpesvirus protein or fragment thereof. Accordingly, the foregoing description of certain preferred embodiments, such as alphavirus VRPs and self-replicating RNAs that contain sequences encoding two or more CMV proteins or fragments thereof, is illustrative of the invention but does not limit the scope of the invention. It will be appreciated that the sequences encoding CMV proteins in such preferred embodiments, can be replaced with sequences encoding proteins, such as gH and gL, or fragments thereof that are 10 amino acids long or longer, from other herpesviruses such as HHV-1, HHV-2, HHV-3, HHV-4, HHV-6, HHV-7 and HHV-8. For example, suitable VZV (HHV-3) proteins include gB, gE, gH, gI, and gL, and fragments thereof that are 10 amino acids long or longer, and can be from any VZV strain. For example, VZV proteins or fragments thereof can be from pOka, Dumas, HJO, CA123, or DR strains of VZV. These exemplary VZV proteins and fragments thereof can be encoded by any suitable nucleotide sequence, including sequences that are codon optimized or deoptimized for expression in a desired host, such as a human cell. Exemplary sequences of VZV proteins are provided herein. 
     For example, in one embodiment, the polycistronic nucleic acid molecule contains a first sequence encoding a VZV gH protein or fragment thereof, and a second sequence encoding a VZV gL protein or fragment thereof. 
     In some embodiments, each of the sequences encoding a herpes virus protein or fragment that are present in the polycistronic nucleic acid molecule is operably linked to its own control elements. For example, each sequences encoding a herpes virus protein or fragment is operably linked to its own subgenomic promoter. Thus the polycistronic nucleic acid molecule, such as an alphavirus replicon, can contain two, three, four or five subgenomic promoters, each of which controls expression of a herpes virus protein or fragment. When this type of polycistronic nucleic acid molecule is a self replicating RNA, such as an alphavirus replicon, it can be packaged as a VRP, or associate or formulated with an RNA delivery system. 
     Methods and Uses 
     In some embodiments, self-replicating RNA molecules or VRPs are administered to an individual to stimulate an immune response. In such embodiments, self-replicating RNA molecules or VRPs typically are present in a composition which may comprise a pharmaceutically acceptable carrier and, optionally, an adjuvant. See, e.g., U.S. Pat. Nos. 6,299,884; 7,641,911; 7,306,805; and US 2007/0207090. 
     The immune response can comprise a humoral immune response, a cell-mediated immune response, or both. In some embodiments an immune response is induced against each delivered CMV protein. A cell-mediated immune response can comprise a Helper T-cell (T h ) response, a CD8+ cytotoxic T-cell (CTL) response, or both. In some embodiments the immune response comprises a humoral immune response, and the antibodies are neutralizing antibodies. Neutralizing antibodies block viral infection of cells. CMV infects epithelial cells and also fibroblast cells. In some embodiments the immune response reduces or prevents infection of both cell types. Neutralizing antibody responses can be complement-dependent or complement-independent. In some embodiments the neutralizing antibody response is complement-independent. In some embodiments the neutralizing antibody response is cross-neutralizing; i.e., an antibody generated against an administered composition neutralizes a CMV virus of a strain other than the strain used in the composition. 
     A useful measure of antibody potency in the art is “50% neutralization titer.” To determine 50% neutralizing titer, serum from immunized animals is diluted to assess how dilute serum can be yet retain the ability to block entry of 50% of viruses into cells. For example, a titer of 700 means that serum retained the ability to neutralize 50% of virus after being diluted 700-fold. Thus, higher titers indicate more potent neutralizing antibody responses. In some embodiments, this titer is in a range having a lower limit of about 200, about 400, about 600, about 800, about 1000, about 1500, about 2000, about 2500, about 3000, about 3500, about 4000, about 4500, about 5000, about 5500, about 6000, about 6500, or about 7000. The 50% neutralization titer range can have an upper limit of about 400, about 600, about 800, about 1000, about 1500, about 200, about 2500, about 3000, about 3500, about 4000, about 4500, about 5000, about 5500, about 6000, about 6500, about 7000, about 8000, about 9000, about 10000, about 11000, about 12000, about 13000, about 14000, about 15000, about 16000, about 17000, about 18000, about 19000, about 20000, about 21000, about 22000, about 23000, about 24000, about 25000, about 26000, about 27000, about 28000, about 29000, or about 30000. For example, the 50% neutralization titer can be about 3000 to about 6500. “About” means plus or minus 10% of the recited value. Neutralization titer can be measured as described in the specific examples, below. 
     An immune response can be stimulated by administering VRPs or self-replicating RNA to an individual, typically a mammal, including a human. In some embodiments the immune response induced is a protective immune response, i.e., the response reduces the risk or severity of CMV infection. Stimulating a protective immune response is particularly desirable in some populations particularly at risk from CMV infection and disease. For example, at-risk populations include solid organ transplant (SOT) patients, bone marrow transplant patients, and hematopoietic stem cell transplant (HSCT) patients. VRPs can be administered to a transplant donor pre-transplant, or a transplant recipient pre- and/or post-transplant. Because vertical transmission from mother to child is a common source of infecting infants, administering VRPs or self-replicating RNA to a woman who can become pregnant is particularly useful. 
     Any suitable route of administration can be used. For example, a composition can be administered intra-muscularly, intra-peritoneally, sub-cutaneously, or trans-dermally. Some embodiments will be administered through an intra-mucosal route such as intra-orally, intra-nasally, intra-vaginally, and intra-rectally. Compositions can be administered according to any suitable schedule. 
     All patents, patent applications, and references cited in this disclosure, including nucleotide and amino acid sequences referred to by accession number, are expressly incorporated herein by reference. The above disclosure is a general description. A more complete understanding can be obtained by reference to the following specific examples, which are provided for purposes of illustration only. 
     Example 1 
     Delivery of Individual CMV Antigens Using a VRP Platform 
     Each of CMV glycoproteins gB and gH induce neutralizing responses, and gB is the dominant antigen among antibodies in human sera that neutralize infection of fibroblasts (Britt et al. (1990) J. Virol. 64(3):1079-85). The following experiments demonstrate in mice a neutralizing response against these antigens delivered using a VRP platform. 
     Each CMV antigen was cloned into a pcDNA-6His vector (Invitrogen) and tested for protein expression before cloning into an alphavirus replicon vector, pVCR 2.1 SalI/XbaI derived from the plasmid described by Perri et al. (J. Virol 77(19)10394-10403 (2003)) producing the constructs shown in  FIG. 2 . pVCR 2.1 SalI/XbaI is a self-replicating RNA vector that, when electroporated with defective helper capsid and glycoprotein RNA, forms an infectious alphavirus particle. 
     pVCR vectors were used to make RNA which was electroporated into baby hamster kidney (BHKV) cells in the presence of defective helper capsid and glycoprotein RNAs derived from Venezuelan equine encephalitis virus (VEE). After electroporation, the supernatant containing secreted alphavirus vector particles (VRPs) was collected, purified, titered, and used for mouse immunization studies. Mice were immunized with 1×10 6  infectious units (IU)/mouse in a series of two immunizations, three weeks apart. The terminal bleed was three weeks after the second immunization. 
     Monocistronic gB, gH and gL VRPs 
     Two different versions of soluble gB were constructed: “gB sol 750” lacks the transmembrane spanning domain and cytoplasmic domain; and “gB sol 692” also lacks a hydrophobic region ( FIG. 2A ) and is similar to the Reap et al. construct. A soluble gH which lacks the transmembrane spanning domain and cytoplasmic domain (“gH sol 716”) was also constructed ( FIG. 2C ). Sera from immunized mice were screened in several assays Immunoblot (data not shown) and immunofluorescence assays were used to confirm specific antibody responses to the antigens. Neutralization assays were used to demonstrate that the elicited antibody responses were able to neutralize CMV infection. 
     Sera from immunized mice were examined by immunofluorescence for recognition of gB in 293T cells transfected with constructs expressing gB-6His. Cells were probed with either anti-His antibodies (“anti-6His”), a monoclonal gB antibody (“anti-gB 27-156”), or collected pooled mouse sera. Pre-immune serum was negative in all cases. In cells transfected with constructs expressing gB FL-6His, fixed, and permeabilized, anti-6His staining revealed an expression pattern of surface expression with a punctate cytoplasmic pattern most likely corresponding to the endocytic/exocytic trafficking pathway. Both anti-gB 27-156 and the pooled mouse sera showed a similar expression pattern. Sera from mice immunized with each of gB FL VRPs, gB sol 750 VRPs, and gB sol 692 VRPs showed the same expression pattern. 
     Mice immunized with gH FL VRPs and gH sol 716 VRPs produced antibodies specific to gH Immunofluorescence analysis of 293T cells transfected with constructs expressing gH FL-6His detected strong recognition of gH by anti-6His, anti-gH, and pooled mouse sera. Sera collected from mice immunized with gL VRPs produced a specific antibody response as determined by immunoblot analysis and immunofluorescence. gL VRPs failed to elicit a neutralizing response. 
     Sera from mice immunized with gB VRPs or gH VRPs were analyzed for the presence of neutralizing antibodies using a CMV neutralization assay. Sera at various dilutions were pre-incubated with CMV virus TB40UL32EGFP (“TB40-GFP,” a clinical isolate engineered to express GFP and then added to ARPE-19 epithelial cells and incubated for 5 days. At 5 days post-infection, the GFP-positive cells were counted. In this assay, cells incubated with serum containing neutralizing antibodies have fewer GFP-positive cells compared to cells incubated with virus alone or with virus incubated with pre-immune sera. Sera from mice immunized with gB VRPs, gB FL VRPs, gB sol 750 VRPs, or gB sol 692 VRPs had strong neutralizing activity in the presence of guinea pig complement (50% neutralization titer at a serum dilution of 1:1280-1:2560;  FIG. 3 ). Sera from mice immunized with gH FL VRPs or gH sol VRPs had some neutralizing activity that was independent of guinea pig complement ( FIG. 3 ). 
     Example 2 
     Construction of Polycistronic Alphavirus Vectors 
     CMV produces several multi-protein complexes during infection. To determine whether a single replicon expressing all components of a desired complex can be used to produce the CMV complex in a subject, or whether components of the complex could be co-delivered from multiple replicon vectors, we designed a platform that allows controlled expression of multiple CMV proteins. 
     An alphavirus vector (pVCR 2.1 SalI/XbaI) was modified to allow assembly of multiple subgenomic promoters (SGP) and genes of interest (GOI). pVCR 2.1SalI/XbaI ApaI site at 11026-31 bp was changed from GGGCCC (SEQ ID NO: 7) to GGCGCC (SEQ ID NO: 8). ClaI and PmlI restriction sites added in the region immediately downstream of the first subgenomic promoter and SalI-XbaI insert sites. The sequence at 7727-7754 bp was changed from ctcgatgtacttccgaggaactgatgtg (SEQ ID NO: 9) to ATCGATGTACTTCCGAGGAACTCACGTG (SEQ ID NO: 10). 
     A shuttling vector system was designed to allow insertion of a GOI directly downstream of a SGP using the SalI-XbaI sites. pcDNA 3.1 (−) C was modified as follows. Three SalI sites were deleted: positions 1046-1051 bp, 3332-3337 bp and 5519-21, 1-3 bp from GTCGAC (SEQ ID NO: 11) to GTCTAC (SEQ ID NO: 12). pcDNA 3.1 (−) C was modified to mutate an XbaI site at position 916-921 bp from TCTAGA (SEQ ID NO: 13) to TCAAGA (SEQ ID NO: 14). pcDNA 3.1 (−) C was modified to add a ClaI site and SacII site at positions 942-947 (ClaI) and 950-955 (SacII) bp from ctggatatctgcag (SEQ ID NO: 15) to ATCGATATCCGCGG (SEQ ID NO: 16). 
     Once the restriction sites were added and the resulting sequence was verified, the region from bp 7611-7689 (ctataactctctacggctaacctgaatggactacgacatagtctagtcgaccaagcctctagacggc gcgcccaccca) (SEQ ID NO: 17) was amplified from the modified pVCR 2.1 alphavirus vector using the following primers 
     
       
         
           
               
               
            
               
                   
                 Forward SGP S-X Not F: 
               
               
                   
                 (SEQ ID NO: 18) 
               
               
                   
                 5′ATAAGAAT GCGGCCGC CTATAACTCTCTACGGCTAACC 3′ 
               
               
                   
               
               
                   
                 Reverse SGP S-X Cla R: 
               
               
                   
                 (SEQ ID NO: 19) 
               
               
                   
                 5′CC ATCGAT TGGGTGGGCGCGCCGTCTAG3′ 
               
               
                   
                 or 
               
               
                   
               
               
                   
                 Forward SGP S-X Cla F: 
               
               
                   
                 (SEQ ID NO: 20) 
               
               
                   
                 5′CC ATCGAT CTATAACTCTCTACGGCTAACC3′ 
               
               
                   
                 and 
               
               
                   
               
               
                   
                 Reverse SGP S-X Sac R: 
               
               
                   
                 (SSEQ ID NO: 21) 
               
               
                   
                 5′TCC CCGCGG TGGGTGGGCGCGCCGTCTAG 3′. 
               
            
           
         
       
     
     The amplified regions were added into the modified pcDNA 3.1(−)C vector to make shuttling vectors (pcDNA SV) between appropriate sites (NotI-ClaI or ClaI-SacII). Insertion of the NotI-SGP Sal-Xba-ClaI forms pcDNA SV cassette 2, insertion of the ClaI-SGP Sal-Xba-SacII forms pcDNA SV cassette 3. These SV cassettes were sequenced. The pcDNA SV cassette 2 contains an additional 12 bp between the XbaI site and the ClaI site (CCACTGTGATCG) (SEQ ID NO: 22) because the ClaI site was not cut in the pcDNA SV cassette 2 vector. A PmlI site was therefore added. For pcDNA SV cassette 2, the PmlI site was inserted at bp 1012 (CACGTG) (SEQ ID NO: 23). For cassette 3, PmlI site was added at bp 935-940 (ACTGTG (SEQ ID NO: 24) was changed to CACGTG (SEQ ID NO: 23). 
     For each polycistronic vector the first gene was inserted directly into the pVCR 2.1 modified vector using the SalI-XbaI sites. The second gene was ligated into pcDNA SV cassette 2 using SalI-XbaI and excised using NotI-PmlI, NotI-SacII or PCRed using primers for NotI-ClaI and digested using NotI and ClaI. The resulting insert SGP-SalI-GOI-Xba was ligated into the modified pVCR 2.1 vector using NotI-PmlI, NotI-SacII, or NotI-ClaI sites. The NotI-ClaI insert was used only when a desired gene in the construct contained a PmlI site. 
     In some cases a third gene was ligated into pcDNA SV cassette 3 using SalI-XbaI and excised using PmlI-SacII or PCRed using primers for ClaI-SacII and digested using ClaI and SacII. The resulting insert SGP-SalI-GOI-XbaI was ligated into the modified pVCR 2.1 using PmlI-SacII or ClaI-SacII. 
     SalI-XbaI digestion was used to validate construction of the polycistronic vector DNA. After digestion with SalI-XbaI, agarose gel electrophoresis was performed to confirm the presence of the GOIs. The polycistronic vector DNA was then linearized with PmeI overnight, purified using Qiagen&#39;s PCR purification kit, and used as template to make RNA using the Ambion mMessage mMachine kit. RNA quality was checked by running a sample aliquot on an RNA agarose gel. 
     Expression from a Polycistronic Vector 
     Fluorescent proteins GFP (green fluorescent protein) and mCherry (red fluorescent protein) were used as the GOIs to assess the ability of the polycistronic vector to express two proteins. We prepared a bicistronic vector in which GFP would be expressed using a first subgenomic promoter and mCherry would be expressed from a second subgenomic promoter ( FIG. 4A ). Polynucleotides containing coding sequences for these proteins were inserted using SalI-XbaI sites. The first polynucleotide (GFP) was inserted directly into the modified alphavirus replicon vector. The second polynucleotide (mCherry) was inserted first into a shuttling vector that contains a subgenomic promoter directly upstream of the coding sequence. A fragment containing both the second subgenomic promoter and the second polynucleotide was isolated and ligated into the modified alphavirus replicon vector containing the first polynucleotide, providing an alphavirus replicon with multiple subgenomic promoters. 
     VRPs were produced in BHKV cells by electroporating replicon RNAs with defective helper RNAs for Cap and Gly. The VRPs were harvested 24 hours after electroporation and used to infect BHKV cells at a multiplicity of infection (MOI) of 20 infectious units (IU) per cell. 
     The experiment tested four sets of VRPs: one VRP expressing only GFP; one VRP expressing mCherry; one VRP expressing only GFP and one VRP expressing only mCherry, both at MOI of 20 IU/cell; and one VRP containing the bicistronic vector GFP(1)-SGPmCherry(2). VRP-infected BHKV cells were examined 24 hours post-infection to determine percent of colocalization. Nearly all the cells were positive for GFP or mCherry when singly infected. Cells infected with two separate VRPs appeared either green or red. Very few cells were yellow, indicating that few cells expressed GFP and mCherry at equal levels and that there was a low level of co-infection. These data were confirmed using FACS analysis ( FIG. 4B ). 
     In contrast, cells infected with alphavirus containing the bicistronic vector GFP(1)-SGPmCherry(2) were all yellow, which indicates approximately equal expression of GFP and mCherry. This study demonstrates that multiple proteins can be expressed successfully from a single polycistronic alphavirus replicon vector. 
     Example 3 
     Production of CMV Complexes 
     This example demonstrates that CMV protein complexes can be formed in a cell after delivery of the complex components from a polycistronic alphavirus replicon vector. 
     gH/gL and gH/gL/gO Complexes 
     Polycistronic gH/gL and gH/gL/gO alphavirus replicons were constructed as described above (shown schematically in  FIG. 5A ). VRPs containing gH, gL, gO, gH/gL and gH/gL/gO encoding replicons were produced in BHKV cells as described above and used to infect BHKV cells to demonstrate complex formation in vitro. VRP infected ARPE-19 cells produced disulfide linked complexes of gH/gL. gO did not detectably alter gH/gL association ( FIG. 5B ). 
     Immunofluorescence studies were conducted to evaluate the localization of gH and gL delivered alone and when delivered using a polycistronic alphavirus to look at relocalization of the proteins when co-expressed. gH localization did not appear to change in the presence or absence of gL, or gL/gO. gL localization did change when in the presence of gH and gH/gO. 
     Finally, gH/gL association was examined via immunoprecipitation. A commercial gH antibody (Genway) was used to investigate the association of gH and gL. In all cases, the gH antibody efficiently immunoprecipitated gH ( FIG. 5C ). When no gH was present, gL was not immunoprecipitated. When gL was expressed in the presence of gH or gH/gO, there was association of gL with gH ( FIG. 5C ). 
     The relocalization of gL in the presence of gH and the association of gH/gL (with or without gO) indicates that all components of the polycistronic alphavirus replicons were expressed and associated to form a complex. 
     Example 4 
     VRPs that Effect gH/gL Complex Formation In Vitro Induce Potent Immune Response to CMV which is Qualitatively and Quantitatively Superior to the Immune Response Elicited to gB VRPs. 
     This example demonstrates the induction of robust immune responses to complexes formed by delivering polycistronic gH/gL VRPs or gH/gL/gO VRPs compared with immune responses obtained using VRPs delivering single components or single-component VRPs administered in combination or to responses elicited by gB VRPs. 
     Mice were infected three times with VRPs administered 3 weeks apart (10 6 IU per mouse; 5 BalbC mice/group). Sera collected from immunizations with single and polycistronic VRPs were screened for neutralizing antibodies using a CMV neutralization assay as described above. Neutralization titer was measured as follows. Various dilutions of sera were pre-incubated with TB40-UL32-EGFP in the presence or absence of guinea pig complement and then added to ARPE-19 epithelial cells or MRC-5 fibroblast cells and incubated for 5 days. After 5 days infection with the virus, GFP-positive cells were counted. Results for the ARPE-19 cells are shown in FIG.  6 A,  FIG. 6B , and  FIG. 6C . Results for the MRC-5 cells are shown in  FIG. 7A  and  FIG. 7B . 
     Sera from mice immunized with gH FL VRPs had low complement-independent neutralizing activity ( FIG. 6A  and  FIG. 6B ). No neutralizing activity was observed using sera from mice immunized with only gL or gO in the presence or absence of guinea pig complement. ( FIG. 6C ) Pooled sera from immunization with several CMV gB proteins (gB FL, gB sol 750, and gB sol 692) demonstrated strong neutralizing activity in the presence of guinea pig complement, with a 50% neutralization titer at 1:1280 sera dilution. However, there was no neutralizing activity in the absence of guinea pig complement in ARPE-19 cells for the pooled gB sera. VRPs expressing single CMV proteins (gH- or gL-VRPs or co-administering gH-, gL-, and gO-VRPs at 10 6  IU/mouse/VRP) did not enhance neutralizing activity beyond that of gH alone. 
     In contrast, sera from mice immunized with bicistronic gH/gL or tricistronic gH/gL/gO VRPs (1×10 6  IU/mouse) demonstrated robust neutralizing responses. Moreover, the responses were similar in the presence and absence of guinea pig complement, showing that polycistronic VRPs successfully induced a complement-independent immune response. ( FIG. 6C .) The 50% neutralization titer was 1:3500-6400+ sera dilution in ARPE-19 cells with TB40-GFP CMV virus. This titer is approximately 3-4 fold higher titer than the 50% complement-dependent neutralization titer for gB pooled sera. 
     Results in the MRC-5 fibroblast cells were similar to those in ARPE-19 cells ( FIGS. 7A and 7B ). Sera from mice immunized with bicistronic gH/gL or tricistronic gH/gL/gO VRPs demonstrated strong neutralizing activity compared to sera from mice immunized with VRPs encoding gH alone, gL alone, or gO alone and to sera from mice immunized by coadministration of gH VRPs and gL VRPs, or coadministration of gH VRPs, gL VRPs, and gO VRPs. These results demonstrate that administration of the polycistronic VRPs induced an immune response that provides good complement-independent neutralization of CMV infection of fibroblast cells. To assess the breadth and potency of the gH/gL immune sera against different strains of CMV, we compared the ability of the sera to block infection of fibroblasts and epithelial cells with six different strains of CMV.  FIG. 8  shows that the gH/gL sera potently neutralize infection of both cell types with a broad range of strains. 
     These data also demonstrate strong neutralizing activity for sera from mice immunized with the polycistronic VRPs but not with mixed pools of VRPs expressing only one protein. This shows that polycistronic replicons that encode the components of a protein complex on a single replicon result in efficient production of the complex in situ. Moreover, because Merlin strain CMV proteins were used to stimulate these responses, the in vitro data obtained using TB40 strain CMV virus demonstrates that the neutralizing antibodies induced by delivery of the polycistronic VRPs are cross-neutralizing antibodies. 
     Example 5 
     RNA Synthesis 
     Plasmid DNA encoding alphavirus replicons (see  FIGS. 14-16 ) served as a template for synthesis of RNA in vitro. Alphavirus replicons contain the genetic elements required for RNA replication but lack those encoding gene products necessary for particle assembly; the structural genes of the alphavirus genome are replaced by sequences encoding a heterologous protein. Upon delivery of the replicons to eukaryotic cells, the positive-stranded RNA is translated to produce four non-structural proteins, which together replicate the genomic RNA and transcribe abundant subgenomic mRNAs encoding the heterologous gene product or gene of interest (GOI). Due to the lack of expression of the alphavirus structural proteins, replicons are incapable of inducing the generation of infectious particles. A bacteriophage (T7 or SP6) promoter upstream of the alphavirus cDNA facilitates the synthesis of the replicon RNA in vitro and the hepatitis delta virus (HDV) ribozyme immediately downstream of the poly(A)-tail generates the correct 3′-end through its self-cleaving activity. 
     In order to allow the formation of an antigenic protein complex, the expression of the individual components of said complex in the same cell is of paramount importance. In theory, this can be accomplished by co-transfecting cells with the genes encoding the individual components. However, in case of non-virally or VRP delivered alphavirus replicon RNAs, this strategy is hampered by inefficient co-delivery of multiple RNAs to the same cell or, alternatively, by inefficient launch of multiple self-replicating RNAs in an individual cell. A potentially more efficient way to facilitate co-expression of components of a protein complex is to deliver the respective genes as part of the same self-replicating RNA molecule. To this end, we engineered alphavirus replicon constructs encoding multiple genes of interest. Every GOI is preceded by its own subgenomic promoter which is recognized by the alphavirus transcription machinery. Thereby, multiple subgenomic messenger RNA species are synthesized in an individual cell allowing the assembly of multi-component protein complexes. 
     Following linearization of the plasmid DNA downstream of the HDV ribozyme with a suitable restriction endonuclease, run-off transcripts were synthesized in vitro using T7 bacteriophage derived DNA-dependent RNA polymerase. Transcriptions were performed for 2 hours at 37° C. in the presence of 7.5 mM of each of the nucleoside triphosphates (ATP, CTP, GTP and UTP) following the instructions provided by the manufacturer (Ambion, Austin, Tex.). Following transcription, the template DNA was digested with TURBO DNase (Ambion, Austin, Tex.). The replicon RNA was precipitated with LiCl and reconstituted in nuclease-free water. Uncapped RNA was capped post-transcripionally with Vaccinia Capping Enzyme (VCE) using the ScriptCap m 7 G Capping System (Epicentre Biotechnologies, Madison, Wis.) as outlined in the user manual. Post-transcriptionally capped RNA was precipitated with LiCl and reconstituted in nuclease-free water. The concentration of the RNA samples was determined by measuring the optical density at 260 nm. Integrity of the in vitro transcripts was confirmed by denaturing agarose gel electrophoresis. 
     Lipid Nanoparticle (LNP) Formulation 
     1,2-dilinoleyloxy-N,N-dimethyl-3-aminopropane (DlinDMA) was synthesized using a previously published procedure [Heyes, J., Palmer, L., Bremner, K., MacLachlan, I. Cationic lipid saturation influences intracellular delivery of encapsulated nucleic acids. Journal of Controlled Release, 107: 276-287 (2005)]. 1, 2-Diastearoyl-sn-glycero-3-phosphocholine (DSPC) was purchased from Genzyme. Cholesterol was obtained from Sigma-Aldrich (St. Lois, Mo.). 1, 2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (PEG DMG 2000), was obtained from Avanti Polar Lipids. 
     LNPs (RV01(14)) were formulated using the following method. 150 μg batch, (PES hollow fibers and no mustang): Fresh lipid stock solutions in ethanol were prepared. 37 mg of DlinDMA, 11.8 mg of DSPC, 27.8 mg of Cholesterol and 8.07 mg of PEG DMG 2000 were weighed and dissolved in 7.55 mL of ethanol. The freshly prepared lipid stock solution was gently rocked at 37° C. for about 15 min to form a homogenous mixture. Then, 453 μL of the stock was added to 1.547 mL ethanol to make a working lipid stock solution of 2 mL. This amount of lipids was used to form LNPs with 150 μg RNA at a 8:1 N:P (Nitrogen to Phosphate) ratio. The protonatable nitrogen on DlinDMA (the cationic lipid) and phosphates on the RNA are used for this calculation. Each μg of self-replicating RNA molecule was assumed to contain 3 nmoles of anionic phosphate, each μg of DlinDMA was assumed to contains 1.6 nmoles of cationic nitrogen. A 2 mL working solution of RNA was also prepared from a stock solution of ˜1 μg/μL in 100 mM citrate buffer (pH 6) (Teknova). Three 20 mL glass vials (with stir bars) were rinsed with RNase Away solution (Molecular BioProducts) and washed with plenty of MilliQ water before use to decontaminate the vials of RNAses. One of the vials was used for the RNA working solution and the others for collecting the lipid and RNA mixes (as described later). The working lipid and RNA solutions were heated at 37° C. for 10 min before being loaded into 3 cc luer-lok syringes (BD Medical). 2 mL of citrate buffer (pH 6) was loaded in another 3 cc syringe. Syringes containing RNA and the lipids were connected to a T mixer (PEEK™ 500 μm ID junction) using FEP tubing ([fluorinated ethylene-propylene] 2 mm ID×3 mm OD, Idex Health Science, Oak Harbor, Wash.). The outlet from the T mixer was also FEP tubing (2 mm ID×3 mm). The third syringe containing the citrate buffer was connected to a separate piece of tubing (2 mm ID×3 mm OD). All syringes were then driven at a flow rate of 7 mL/min using a syringe pump (from kdScientific, model no. KDS-220). The tube outlets were positioned to collect the mixtures in a 20 mL glass vial (while stirring). The stir bar was taken out and the ethanol/aqueous solution was allowed to equilibrate to room temperature for 1 h. Then the mixture was loaded in a 5 cc syringe (BD Medical), which was fitted to a piece of FEP tubing (2 mm ID×3 mm OD) and in another 5 cc syringe with equal length of FEP tubing, an equal volume of 100 mM citrate buffer (pH 6) was loaded. The two syringes were driven at 7 mL/min flow rate using a syringe pump and the final mixture collected in a 20 mL glass vial (while stirring). Next, LNPs were concentrated to 2 mL and dialyzed against 10-15 volumes of 1×PBS (from Teknova) using the Tangential Flow Filtration (TFF) system before recovering the final product. The TFF system and hollow fiber filtration membranes were purchased from Spectrum Labs and were used according to the manufacturer&#39;s guidelines. Polyethersulfone (PES) hollow fiber filtration membranes (part number P-C1-100E-100-01N) with a 100 kD pore size cutoff and 20 cm 2  surface area were used. For in vitro and in vivo experiments, formulations were diluted to the required RNA concentration with 1×PBS (from Teknova). 
     Particle Size 
     Particle size was measured using a Zetasizer Nano ZS (Malvern Instruments, Worcestershire, UK) according to the manufacturer&#39;s instructions. Particle sizes are reported as the Z average with the polydispersity index (pdi). Liposomes were diluted in 1×PBS before measurement. 
     Encapsulation Efficiency and RNA Concentration 
     The percentage of encapsulated RNA and RNA concentration were determined by Quant-iT RiboGreen RNA reagent kit (Invitrogen). Manufacturer&#39;s instructions were followed in the assay. The ribosomal RNA standard provided in the kit was used to generate a standard curve. LNPs either obtained from method 1 or methods 2-5 were diluted ten fold or one hundred fold respectively in 1× TE buffer (from kit), before addition of the dye. Separately, LNPs were diluted ten or 100 fold in 1× TE buffer containing 0.5% Triton X (Sigma-Aldrich), before addition of the dye. Thereafter an equal amount of dye was added to each solution and then ˜180 μL of each solution after dye addition was loaded in duplicate into a 96 well tissue culture plate (obtained from VWR, catalog #353072). The fluorescence (Ex 485 nm, Em 528 nm) was read on a microplate reader (from BioTek Instruments, Inc.). 
     Triton X was used to disrupt the LNPs, providing a fluorescence reading corresponding to the total RNA amount and the sample without Triton X provided fluorescence corresponding to the unencapsulated RNA. % RNA encapsulation was determined as follows: LNP RNA Encapsulation (%)=[(F t −F i )/F t ]×100, where F t  is the fluorescence intensity of LNPs with triton X addition and F i  is the fluorescence intensity of the LNP solution without detergent addition. These values (F t  and F i ) were obtained after subtraction from blank (1×TE buffer) fluorescence intensity. The concentration of encapsulated RNA was obtained by comparing F t −F i  with the standard curve generated. All LNP formulations were dosed in vivo based on the encapsulated dose. 
     Viral Replicon Particles (VRP) 
     To compare RNA vaccines to traditional RNA-vectored approaches for achieving in vivo expression of reporter genes or antigens, we utilized viral replicon particles (VRPs), produced in BHK cells by the methods described by Perri et al. (J. Virol 77(19):10394-10403 (2003)), coding for expression of the same antigens as the corresponding RNA constructs. In this system, the antigen consisted of alphavirus chimeric replicons (VCR) derived from the genome of Venezuelan equine encephalitis virus (VEEV) engineered to contain the 3′ terminal sequences (3′ UTR) of Sindbis virus and a Sindbis virus packaging signal (PS) (see FIG. 2 of Perri et al). The replicons were packaged into VRPs by co-electroporating them into baby hamster kidney (BHK) cells along with defective helper RNAs encoding the Sindbis virus capsid and glycoprotein genes (see FIG. 2 of Perri et al). The VRPs were then harvested and partially purified by ultracentrifugation on a sucrose cushion and concentrated on an Amicon concentrator. The resulting VRP stock was titrated by standard methods and inoculated into animals in culture fluid or other isotonic buffers. An alphavirus replicon particle chimera derived from venezuelan equine encephalitis and sindbis viruses is a potent gene-based vaccine delivery vector.  J. Virol.  77, 10394-10403. 
     Murine Immunogenicity Studies 
     Groups of 10 female BALB/c mice aged 8-10 weeks and weighing about 20 g were immunized with 1×10 6  IU (VRP) or 1.0 μg (RNA) at day 0, 21 and 42 with bleeds taken 3 weeks after the 2 nd  and 3 weeks after the 3 rd  vaccinations. All animals were injected in the quadriceps in the two hind legs each getting an equivalent volume (50 μl per site). 
     Micro Neutralization Assay 
     Serum samples were tested for the presence of neutralizing antibodies by an infection reduction neutralization test. Two-fold serial dilutions of HI-serum (in DMEM with 10% HI FBS) were added to an equal volume of CMV (strain TB40 or clinical isolate 8819) previously titered to give approximately 200 IU/50 μl. The VR1814, Towne, AD169 strains and the clinical isolate 8822 were also used. Serum/virus mixtures were incubated for 2 hours at 37° C. and 5% CO2, to allow virus neutralization to occur, and then 50 μl of this mixture (containing approximately 200 IU) was inoculated on duplicate wells of ARPE-19 cells in 96 half well plates. Plates were incubated for 40-44 hours. Unless otherwise noted, the number of positive infected foci was determined by immunostaining with an AlexaFluor 488 conjugated IE1 CMV monoclonal antibody followed by automated counting. The neutralization titer is defined as the reciprocal of the serum dilution producing a 50% reduction in number of positive virus foci per well, relative to controls (no serum). 
     Immunogenicity of gH/gL VRPs and LNP Formulated RNA 
     The A323 replicon that expresses the surface glycoprotein B (gB) of CMV, the A160 replicon that expresses the membrane complex of the full-length glycoprotein H and L (gH/gL) and the A322 replicon that expresses the membrane complex of the soluble form of glycoprotein H and L (gHsol/gL) were used for this experiment. BALB/c mice, 10 animals per group, were given bilateral intramuscular vaccinations (50 μL per leg) on days 0, 21 and 42 with VRPs expressing gB (1×10 6  IU), VRPs expressing gH/gL (1×10 6  IU), VRP&#39;s expressing gHsol/gL (1×10 6  IU) and PBS as the controls. The three test groups received self-replicating RNA (A160, A322 or A323) formulated in LNP (RV01(14). Serum was collected for immunological analysis on days 39 (3wp2) and 63 (3wp3). 
     Results 
     The sive and percentage of encapsulated RNA in the RV01(14) formulations made for the experiment are shown in Table 3. 
     
       
         
           
               
               
               
               
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
               
                   
                   
                   
                 pKa of 
                 Particle 
                   
                   
               
               
                   
                 Lipid Composition  
                   
                 cationic 
                 Size Zav 
                   
                 Percent RNA 
               
               
                 RV# 
                 (% moles of total) 
                 RNA 
                 lipid 
                 (nm) 
                 pdI 
                 Encapsulation 
               
               
                   
               
             
            
               
                 RV01 (14) 
                 DlinDMA 40%, DSPC- 
                 gB FL 
                 5.8 
                 170 
                 0.098 
                 88.3 
               
               
                   
                 10%, Chol- 48%, PEG 
                   
                   
                   
                   
                   
               
               
                   
                 DMG 2k-2% 
                   
                   
                   
                   
                   
               
               
                 RV01 (14) 
                 DlinDMA 40%, DSPC- 
                 gH FL/gL 
                 5.8 
                   168.8 
                 0.144 
                 87.4 
               
               
                   
                 10%, Chol- 48%, PEG 
                   
                   
                   
                   
                   
               
               
                   
                 DMG 2k-2% 
                   
                   
                   
                   
                   
               
               
                 RV01 (14) 
                 DlinDMA 40%, DSPC- 
                 gHsol/gL 
                 5.8 
                 162 
                 0.131 
                 90   
               
               
                   
                 10%, Chol- 48%, PEG 
                   
                   
                   
                   
                   
               
               
                   
                 DMG 2k-2% 
               
               
                   
               
            
           
         
       
     
     The 50% neutralizing titers for the terminal sera (day 63, three weeks after final vaccination) are shown in Table 4. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                   
                   
                 ARPE-19, HCMV TB40 
                 ARPE-19, HCMV 8819 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                   
                 pool #1 
                 pool #2 
                 average 
                 pool #1 
                 pool #2 
                 average 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Preimmune serum 
                 — 
                 126 
                 212 
                 169 
                 50 
                 50 
                 50 
               
               
                 gB FL VRP 
                 10 6  IU 
                 1332 
                 295 
                 814 
                 5085 
                 1031 
                 3058 
               
               
                 gB FL RNA-RV01(14) 
                 1 μg 
                 686 
                 179 
                 433 
                 1261 
                 557 
                 909 
               
               
                 gH FL/gL VRP 
                 10 6  IU 
                 1425 
                 1624 
                 1525 
                 2496 
                 1374 
                 1935 
               
               
                 gH FL/gL RNA- 
                 1 μg 
                 6196 
                 6390 
                 6293 
                 5800 
                 10267 
                 8034 
               
               
                 RV01(14) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 gH sol/gL VRP 
                 10 6  IU 
                 2375 
                 2254 
                 2315 
                 1733 
                 1924 
                 1829 
               
               
                 gH sol/gL RNA- 
                 1 μg 
                 4600 
                 2062 
                 3331 
                 2912 
                 1533 
                 2223 
               
               
                 RV01(14) 
               
               
                   
               
            
           
         
       
     
     RNA expressing either a full-length or a presumed soluble form of the HCMV gH/gL complex elicit high titers of neutralizing antibody, as assayed on epithelial cells using two different HCMV strains. The average titers elicited by the gH/gL RNAs are at least as high as the average titer for the corresponding gH/gL VRPs (see  FIG. 17 ). 
     Example 6 Bicistronic and Pentacistronic Nucleic Acids Encoding CMV Proteins 
     Additional bicistronic and pentacistronic alphavirus replicons that express glycoprotein complexes from human cytomegalovirus (HCMV) were prepared, and are shown schematically in  FIGS. 18 and 20 . The alphavirus replicons were based on venezuelan equine encephalitis virus (VEE). The replicons were packaged into viral replicon particles (VRPs), encapsulated in lipid nanoparticles (LNP), or formulated with a cationic nanoemulsion (CNE). Expression of the encoded HCMV proteins and protein complexes from each of the replicons was confirmed by immunoblot, co-immunoprecipitation, and flow cytometry. Flow cytometry was used to verify expression of the pentameric gH/gL/UL128/UL130/UL131 complex from pentameric replicons encoding the protein components of the complex, using human monoclonal antibodies specific to conformational epitopes present on the pentameric complex (Macagno et al (2010), J. Virol. 84(2):1005-13).  FIG. 19  shows that these antibodies bind to BHKV cells transfected with replicon RNA expressing the HCMV gH/gL/UL128/UL130/UL131 pentameric complex (A527). Similar results were obtained when cells were infected with VRPs made from the same replicon construct. This shows that replicons designed to express the pentameric complex do indeed express the desired antigen and not the potential byproduct gH/gL. 
     The VRPs, RNA encaspulated in LNPs, and RNA formulated with CNE were used to immunize Balb/c mice by intramuscular injections in the rear quadriceps. The mice were immunized three times, three weeks apart, and serum samples were collected prior to each immunization as well as three weeks after the third and final immunization. The sera were evaluated in microneutralization assays to measure the potency of the neutralizing antibody response that was elicited by the vaccinations. The titers are expressed as 50% neutralizing titer. 
     The immunogenicity of a number of different configurations of a bicistronic expression cassette for a soluble HCMV gH/gL complex in VRPs was assessed.  FIG. 20  shows that VRPs expressing the membrane-anchored, full-length gH/gL complex elicited potent neutralizing antibodies at slightly higher titers than the soluble complex (gHsol/gL) expressed from a similar bicistronic expression cassette. Changing the order of the genes encoding gHsol and gL or replacing one of the subgenomic promoters with an IRES or an FMDV 2A site did not substantially improve immunogenicity. 
     The breadth and potency of HCMV neutralizing activity in sera from mice immunized with VEE/SIN VRPs expressing gH/gL was assessed by using the sera to block infection of fibroblasts and epithelial cells with different strains of HCMV. Table 5 shows that gH/gL immune sera were broadly and potently neutralizing against six different strains of HCMV on both cell types in the absence of complement. Addition of complement had a slight negative effect on the neutralizing potency of the sera. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Neutralizing antibody titers in sera from mice immunized 
               
               
                 with pVCR-derived VRPs expressing gH/gL. 
               
            
           
           
               
               
            
               
                   
                 Serum from mice immunized with pVCR- 
               
               
                   
                 derived VRPs expressing gH/gL 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Without 
                 With 
               
               
                 HCMV Strain 
                 Cell 
                 complement 
                 complement 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 Towne 
                 Fibroblasts 
                 5244 
                 4081 
               
               
                 AD169 
                 (MRC-5) 
                 2126 
                 2208 
               
               
                 TB40-UL32-EGFP 
                   
                 678 
                 505 
               
               
                 VR1814 
                   
                 4764 
                 2126 
               
               
                 TB40-UL32-EGFP 
                 Epithelial cells 
                 5602 
                 3247 
               
               
                 VR1814 
                 (ARPE-19) 
                 6510 
                 2420 
               
               
                 8819 (clinical isolate) 
                   
                 8706 
                 5242 
               
               
                 8822 (clinical isolate) 
                   
                 3427 
                 2684 
               
               
                   
               
            
           
         
       
     
     The immunogenicity of LNP-encapsulated RNAs encoding the pentameric complex (A526 and A527) compared to LNP-encapsulated RNA (A160) and VRPs (pVCR modified gH-SGPgL) expressing gH/gL was assessed. Table 6 shows that replicons expressing the pentameric complex elicited more potently neutralizing antibodies than replicons expressing gH/gL. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Neutralizing antibody titers. 
               
            
           
           
               
               
               
               
            
               
                   
                 Titer 
                 Titer 
                 Titer 
               
               
                 Replicon 
                 post 1 st   
                 post 2 nd   
                 post 3 rd   
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 C313 pVCR modified gH-SGP-gL 
                 126 
                 6,296 
                 26,525 
               
               
                 VRP 10 6  IU 
                   
                   
                   
               
               
                 A160 gH FL/gL 1 μg LNP 
                 347 
                 9,848 
                 42,319 
               
               
                 A526 Pentameric 2A 1 μg LNP 
                 179 
                 12,210 
                 80,000 
               
               
                 A527 Pentameric IRES 1 μg LNP 
                 1,510 
                 51,200 
                 130,000 
               
               
                   
               
            
           
         
       
     
     The pentacistronic VEE-based RNA replicon that elicited the highest titers of neutralizing antibodies (A527) was packaged as VRPs and the immunogenicity of the VRPs were compared to gH/gL-expressing VRPs and LNP-encapsulated replicons expressing gH/gL and pentameric complex. Table 7 shows that VRPs expressing the pentameric complex elicited higher titers of neutralizing antibodies than VRPs expressing gH/gL. Moreover, 10 6  infectious units of VRPs are at least as potent as 1 μg of LNP-encapsulated RNA when the VRPs and the RNA encoded the same protein complexes. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Neutralizing antibody titers. Sera were collected three weeks after the second 
               
               
                 immunization. 
               
            
           
           
               
               
            
               
                 Replicon 
                 50% Neutralizing Titer 
               
               
                   
               
            
           
           
               
               
            
               
                 A160 gH FL/gL VRP 10 6  IU 
                 14,833 
               
               
                 A527 Pentameric IRES VRP 10 6  IU 
                 51,200 
               
               
                 A160 gH FL/gL LNP 0.01 μg 
                 4,570 
               
               
                 A160 gH FL/gL LNP 0.1 μg 
                 9,415 
               
               
                 A160 gH FL/gL LNP 1 μg 
                 14,427 
               
               
                 A527 Pentameric IRES 0.01 μg LNP 
                 12,693 
               
               
                 A527 Pentameric IRES 0.1 μg LNP 
                 10,309 
               
               
                 A527 Pentameric IRES 1 μg LNP 
                 43,157 
               
               
                   
               
            
           
         
       
     
     The breadth and potency of HCMV neutralizing activity in sera from mice immunized with VEE-based RNA encoding the pentameric complex (A527) was assessed by using the sera to block infection of fibroblasts and epithelial cells with different strains of HCMV. Table 8 shows that anti-gH/gL/UL128/UL130/UL131 immune sera broadly and potently neutralized infection of epithelial cells. This effect was complement independent. In contrast, the sera had a reduced or not detectable effect on infection of fibroblasts. These results are what is expected for immune sera that contains mostly antibodies specific for the gH/gL/UL128/UL130/UL131 pentameric complex, because the pentameric complex is not required for infection of fibroblasts and, consequently, antibodies to UL128, UL130, and UL131 do not block infection of fibroblasts (Adler et al (2006), J. Gen. Virol. 87(Pt. 9):2451-60; Wang and Shenk (2005), Proc. Natl. Acad. Sci. USA 102(50):18153-8). Thus, these data demonstrate that the pentameric replicons encoding the gH/gL/UL128/UL130/UL131pentameric complex specifically elicit antibodies to the complex in vivo. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Neutralizing antibody titers in sera from mice immunized 
               
               
                 with the A527 RNA replicon encapsulated in LNPs. 
               
               
                 The replicon expresses the HCMV pentameric complex 
               
               
                 using subgenomic promoters and IRESes. 
               
            
           
           
               
               
            
               
                   
                 Serum from mice immunized with A527 
               
               
                   
                 pentameric IRES RNA in LNPs 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Without 
                 With 
               
               
                 HCMV Strain 
                 Cell 
                 complement 
                 complement 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 Towne 
                 Fibroblasts 
                 3433 
                 1574 
               
               
                 AD169 
                 (MRC-5) 
                 2292 
                 &lt;1000 
               
               
                 TB40-UL32-EGFP 
                   
                 &lt;1000 
                 &lt;1000 
               
               
                 VR1814 
                   
                 4683 
                 1324 
               
               
                 TB40-UL32-EGFP 
                 Epithelial cells 
                 86991 
                 59778 
               
               
                 VR1814 
                 (ARPE-19) 
                 82714 
                 37293 
               
               
                 8819 (clinical isolate) 
                   
                 94418 
                 43269 
               
               
                 8822 (clinical isolate) 
                   
                 85219 
                 49742 
               
               
                   
               
            
           
         
       
     
     To see if bicistronic and pentacistronic replicons expressing the gH/gL and pentameric complexes would elicit neutralizing antibodies in different formulations, cotton rats were immunized with bicistronic or pentacistronic replicons mixed with a cationic nanoemulsion (CNE). Table 9 shows that replicons in CNE elicited comparable neutralizing antibody titers to the same replicons encapsulated in LNPs. 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Neutralizing antibody titers. The sera were collected three weeks  
               
               
                 after the second immunization. 
               
            
           
           
               
               
               
            
               
                   
                 Replicon 
                 50% Neutralizing Titer 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 A160 gH FL/gL VRP 10 6  IU 
                 594 
               
               
                   
                 A160 gH FL/gL 1 μg LNP 
                 141 
               
               
                   
                 A527 Pentameric IRES 1 μg LNP 
                 4,416 
               
               
                   
                 A160 gH FL/gL 1 μg CNE 
                 413 
               
               
                   
                 A527 Pentameric IRES 1 μg CNE 
                 4,411 
               
               
                   
                   
               
            
           
         
       
     
     Example 7. Replicons Encoding VZV Proteins 
     Nucleic acids encoding VZV proteins were cloned into a VEE replicon vector to produce monocystronic replicons that encode gB, gH, gL, gE, and gI, and to produce bicistronic replicons that encode gH/gL or gE/gI. In the bicistronic replicons, expression of each VZV open reading frame was driven by a separate subgenomic promoter. 
     To prepare replicon RNA, plasmid encoding the replicon was linearized by digestion with PmeI, and the linearized plasmid was extracted with phenol/chloroform/isoamylalchohol, precipitated in sodium acetate/ethanol and resuspended in 20 μl of RNase-free water. 
     RNA was prepared by In vitro transcription of 1 μg of linearized DNA using the MEGAscript T7 kit (AMBION #AM1333). A 20 μl reaction was set up according to the manufacturer&#39;s instruction without cap analog and incubated for 2 hours at 32° C. TURBO DNase (1 μl) was added and the mixture was incubate for 30 min. at 32° C. RNase-free water (30 μl) and ammonium acetate solution (30 μl) were added. The solution was mixed and chilled for at least 30 min at −20° C. Then the solution was centrifuged at maximum speed for 25 min. at 4° C. The supernatant was discarded, and the pellet was rinsed with 70% ethanol, and again centrifuged at maximum speed for 10 min. at 4° C. The pellet was air dried and resuspended in 50 μl of RNase-free water. The concentration of RNA was measured and quality was check on a denaturing gel. 
     The RNA was capped using the ScriptCap m7G Capping System (Epicentre #SCCE0625). The reaction was scaled by combining the RNA and RNase-free water. The RNA was then denatured for 5-10 min at 65° C. The denatured RNA was transfered quickly to ice and the following reagents were added in the following order: ScriptCap Capping Buffer, 10 mM GTP, 2 mM SAM fresh prepared, ScriptGuard RNase inhibitor, and ScriptCap Capping Enzyme. The mixture was incubated for 60 min at 37° C. The reaction was stopped by adding RNase-free water and 7.5 M LiCl, mixing well and storing the mixture for at least 30 min at −20° C. Then, the mixture was centrifuged at maximum speed for 25 min. at 4° C., the pellet was rinsed with 70% ethanol, again centrifuged at maximum speed for 10 min. at 4° C. and the pellet was air dried. The pellet was resuspended in RNase-free water. The concentration of RNA was measured and quality was checked on a denaturing gel. 
     RNA Transfection 
     Cells (BHK-V cells) were seeded on 6-well plates brought to 90-95% confluence at the time of transfection. For each transfection 3 μg of RNA was diluted in 50 mL OPTIMEM media in a first tube. Lipofectamine 2000 was added to a second tube contained 50 mL OPTIMEM media. The firs and second tubes were combined and kept for 20 mM at room temperature. The culture media in the 6-well plates were replaced with fresh media, and the RNA-Lipofectamine complex was placed onto the cells, and mixed by gently rocking the plate. The plates were incubated for 24 hours at 37° C. in a CO 2  incubator. 
     Expression of the VZV proteins in transfected cells was assessed by western blot and immunofluorescence. For western blots, lysates of transfected cells were separated by electrophoresis (5 μg total proteins/lane) and blotted. A cleared viral suspension (7 μg total protein/lane) derived from the OKA/Merck vaccine strain was used as a positive control. Blots were probed using commercially available antibodies (1:1000 dilution) that bind VZV proteins. 
     For immunofluorescence, transfected cells were harvested and seeded in 96 well plate, and intracellular staining was performed using commercially available mouse mAbs (dilution range 1:100 1:400). Cell pellets were fixed and permeabilized with Citofix-Citoperm solutions. A secondary reagent, Alexa488 labelled goat anti-mouse F(ab′) 2  (1:400 final dilution), was used. 
     Expression of VZV proteins gE and gI was detected in cells transfected with monocistronic constructs (gE or gI), and expression of both gE and gI was detected in cells transfected with a bicistronic gE/gI construct in western blots using commercially available mouse antibodies, 13B1 for gE and 8C4 for gI. Expression of VZV protein gB was detected in cells transfected with a monocistronic construct encoding gB, by immunofluorescence using commercially available antibody 10G6. Expression of the VZV protein complex gH/gL, was detected by immunofluorescence in cells transfected with monocistronic gH and monocistronic gL, or with a bicistronic gH/gL construct. The gH/gL complex was detected using commercially available antibody SG3. 
     Murine Immunogenicity Studies 
     Groups of 8 female BALB/c mice aged 6-8 weeks and weighing about 20 g were immunized intramuscularly with 7.0 or 1.0 μg of replicon RNA formulated with a CNE or LNP (RV01) at day 0, 21 and 42. Blood samples were taken from the immunized animals 3 weeks after the 2nd immunization and 3 weeks after the 3rd immunization. The groups are shown in Table 10. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 10 
               
               
                   
               
               
                   
                   
                 Dose 
                   
               
               
                 Group 
                 Antigen 
                 (micrograms) 
                 Formulation 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Study 1 
               
            
           
           
               
               
               
               
            
               
                 1 
                 YFP 
                 7 
                 CNE 
               
               
                 2 
                 YFP 
                 1 
                 CNE 
               
               
                 3 
                 gB 
                 7 
                 CNE 
               
               
                 4 
                 gB 
                 1 
                 CNE 
               
               
                 5 
                 gE 
                 7 
                 CNE 
               
               
                 6 
                 gE 
                 1 
                 CNE 
               
               
                 7 
                 gH 
                 7 
                 CNE 
               
               
                 8 
                 gH 
                 1 
                 CNE 
               
               
                 9 
                 gI 
                 7 
                 CNE 
               
               
                 10 
                 gI 
                 1 
                 CNE 
               
               
                 11 
                 gL 
                 7 
                 CNE 
               
               
                 12 
                 gL 
                 1 
                 CNE 
               
               
                 13 
                 gE/gI 
                 7 
                 CNE 
               
               
                 14 
                 gE/gI 
                 1 
                 CNE 
               
               
                 15 
                 gH/gL 
                 7 
                 CNE 
               
               
                 16 
                 gH/gL 
                 1 
                 CNE 
               
            
           
           
               
            
               
                 Study 2 
               
            
           
           
               
               
               
               
            
               
                 1 
                 gB 
                 1 
                 RV01 
               
               
                 2 
                 gE 
                 1 
                 RV01 
               
               
                 3 
                 gH 
                 1 
                 RV01 
               
               
                 4 
                 gI 
                 1 
                 RV01 
               
               
                 5 
                 gL 
                 1 
                 RV01 
               
               
                 6 
                 gE/gI 
                 1 
                 RV01 
               
               
                 7 
                 gH/gL 
                 1 
                 RV01 
               
               
                   
               
            
           
         
       
     
     Immune Response to VZV Antigens 
     Serum samples were tested for the presence of antibodies to gB, by intracellular staining of VZV-replicon transfected MRC-5 cells. MRC-5 cells were maintained in Dulbecco Modified Eagle&#39;s Medium with 10% fetal bovine serum. VZV Oka strain inoculum (obtained from ATCC) was used to infect MRC-5 cell culture and infected whole cells were used for subpassage of virus. The ratio between infected and un-infected cells was 1:10. 30 hrs post infection cells were trypsin-dispersed for seeding in a 96 well plate to perform an intracellular staining with pools of mice sera (dilution range 1:200 to 1:800) obtained after immunization. Commercial mAbs were used as controls to quantify the infection level. Cell pellets ware fixed and permeabilized with Citofix-Citoperm solutions. A secondary reagent, Alexa488 labelled goat anti-mouse F(ab′)2 was used (1:400 final dilution). 
     Commercial antibodies to gB (10G6), gH (SG3), and gE (13B1 (SBA) and 8612 (Millipore)) were used as positive controls, and each intracellularly stained infected MRC-5 cells Immune sera obtained 3 weeks after the third immunization with either 1 or 7 μg of RNA formulated with CNE or LNP were diluted 1/200, 1/400 and 1/800 and used to intracellulary stain infected MRC-5 cells. The results are shown in  FIG. 21  (Study 1, groups 1, 5, 7, 9, 11, 13 and 15, CNE formulation) and  FIG. 22  (Study 2, groups 1-7, LNP formulation). 
     Neutralizing Assay 
     Each immunized mouse serum was serially diluted by two fold increments starting at 1:20 in standard culture medium, and added to the equal volume of VZV suspension in the presence of guinea pig complement. After incubation for 1 hour at 37° C., the human epithelial cell line A549, was added. Infected cells can be measured after one week of culture by counting plaques formed in the culture under microscope. From the plaque number the % inhibition at each serum dilution was calculated. A chart for each serum sample was made by plotting the value of % inhibition against the logarithmic scale the dilution factor. Subsequently an approximate line of relationship between dilution factor and % inhibition was drawn. Then the 50% neutralization titer was determined as the dilution factor where the line crossed at the value of 50% inhibition. 
     Table 11 shows that sera obtained from mice immunized with monocistronic gE, bicistrnic gE/gI, and bicistronic gH/gL contained robust neutralizing antibody titers. 
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Neutralization titers of pooled sera from mice  
               
               
                 immunized with 7 μg RNA 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Control 
                   
                   
                   
                   
                   
                   
                   
               
               
                 (YFP) 
                 gB 
                 gE 
                 gI 
                 gE/gI 
                 gH 
                 gL 
                 gH/gL 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 &lt;20 
                 &lt;20 
                 1111 
                 &lt;20 
                  440 
                 &lt;20 
                 &lt;20 
                  1070 
               
               
                 &lt;20 
                 &lt;20 
                  413 
                  51 
                 &gt;2560  
                 &lt;20 
                 &lt;20 
                 &gt;2560 
               
               
                 &lt;20 
                 &lt;20 
                 &gt;2560  
                 &lt;20 
                 1031 
                 &lt;20 
                 &lt;20 
                 &gt;2560 
               
               
                 &lt;20 
                  20 
                 2128 
                 &lt;20 
                 1538 
                 &lt;20 
                 &lt;20 
                 &gt;2560 
               
               
                 &lt;20 
                  20 
                  861 
                 &lt;20 
                  636 
                  20 
                 &lt;20 
                 &gt;2560 
               
               
                 &lt;20 
                 &lt;20 
                 1390 
                 &lt;20 
                 2339 
                 &lt;20 
                 &lt;20 
                 &gt;2560 
               
               
                 &lt;20 
                 &lt;20 
                  969 
                 &lt;20 
                 1903 
                 &lt;20 
                 &lt;20 
                  900 
               
               
                 &lt;20 
                 &lt;20 
                 1011 
                  20 
                 1969 
                  20 
                 &lt;20 
                 &gt;2560 
               
               
                  &lt;20* 
                  &lt;20* 
                   &lt;20* 
                  &lt;20* 
                  &lt;20* 
                  &lt;20* 
                  &lt;20* 
                   &lt;20* 
               
               
                   
               
               
                 *pre-immune pooled sera 
               
            
           
         
       
     
     REFERENCES 
     
         
         Britt W J, Alford C A. Cytomegalovirus. In Fields B N, Knipe D M, Howley P M (ed.). Fields Virology, 3 rd  edition, Philadelphia, Pa.: Lippincott/Raven; 1996. p. 2493-523. 
         Chee M S, Bankier A T, Beck S, Bohni R, Brown C M, Cerny R, Horsnell T, Hutchinson C A, Kouzarides T, Martignetti J A, Preddie E, Satchwell S C, Tomlinson P, Weston K M and Barrell B G. 1990. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr. Top. Microbiol. Immunol. 154:125-70. 
         Davison A J, Dolan A, Akter P, Addison C, Dargan D J, Alcendor D J, McGeoch D J and Hayward G S. 2003. The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome. J. Gen. Virol. 84:17-28. (Erratum, 84:1053). 
         Crumpacker C S and Wadhwa S. 2005. Cytomegalovirus, p 1786-1800. In G. L. Mandell, J. E. Bennett, and R. Dolin (ed.), Principles and practice of infectious diseases, vol 2. Elsevier, Philadelphia, Pa. 
         Pomeroy C and Englund J A. 1987. Cyotmegalovirus: epidemiology and infection control. Am J Infect Control 15: 107-119. 
         Murphy E, Yu D, Grimwood J, Schmutz J, Dickson M, Jarvis M A, Nelson J A, Myers R M and Shenk T E. 2003. Coding potential of laboratory and clinical strains of cytomegalovirus. Proc. Natl. Acad. Sci. USA 100:14976-81. 
         Mocarski E S and Tan Courcelle C. 2001. Cytomegalovirus and their replication, p. 2629-73. In D M Knipe and P M Howley (ed.) Fields Virology, 4 th  edition, vol. 2. Lippincott Williams and Wilkins, Philadelphia, Pa. 
         Compton T. 2004. Receptors and immune sensors: the complex entry path of human cytomegalovirus. Trends Cell. Bio. 14(1): 5-8. 
         Britt W J and Alford C A. 2004. Human cytomegalovirus virion proteins. Hum. Immunol. 65:395-402. 
         Varnum S M, Streblow D N, Monroe M E, Smith P, Auberry K J, Pasa-Tolic L, Wang D, Camp II D G, Rodland K, Wiley, Britt W, Shenk T, Smith R D and Nelson J A. 2004. Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome. J. Virol. 78:10960-66. (Erratum, 78:13395). 
         Ljungman P, Griffiths P and Paya C. 2002. Definitions of cytomegalovirus infection and disease in transplant recipients. Clin. Infect. Dis. 34:1094-97. 
         Rubin R. 2002. Clinical approach to infection in the compromised host, p. 573-679. In R. Rubin and L S Young (ed), Infection in the organ transplant recipient. Kluwer Academic Press, New York, N.Y. 
         Stagno S and Britt W J. 2005. Cytomegalovirus, p. 389-424. In J S Remington and J O Klein (ed), Infectious diseases of the fetus and newborn infant, 6htt edition. W B Saunders, Philadelphia, P A. 
         Britt W J, Vugler L, Butfiloski E J and Stephens E B. 1990. Cell surface expression of human cytomegalovirus (HCMV) gp55-116 (gB): use of HCMV-vaccinia recombinant virus infected cells in analysis of the human neutralizing antibody response. J. Virol. 64:1079-85. 
         Reap E A, Dryga S A, Morris J, Rivers B, Norberg P K, Olmsted R A and Chulay J D. 2007. Cellular and Humoral Immune Responses to Alphavirus Replicon Vaccines expressing Cytomegalovirus pp65, IL1 and gB proteins. Clin. Vacc. Immunol. 14:748-55. 
         Balasuriya U B R, Heidner H W, Hedges J F, Williams J C, Davis N L, Johnston R E and MacLachlan N J. 2000. Expression of the two major envelope proteins of equine arteritis virus as a heterodimer is necessary for induction of neutralizing antibodies in mice immunized with recombinant Venezuelan equine encephalitis virus replicon particles. J. Virol. 74:10623-30. 
         Dunn W, Chou C, Li H, Hai R, Patterson D, Stoic V, Zhu H and Liu F. 2003. Functional profiling of a human cytomegalovirus genome. Proc. Natl. Acad. Sci USA 100:14223-28. 
         Hobom U, Brune W, Messerle M, Hahn G and Kosinowski U H. 2000. Fast screening procedures for random transposon libraries of cloned herpesvirus genomes: mutational analysis of human cytomegalovirus envelope glycoprotein genes. J. Virol. 74:7720-29. 
         Ryckman B J, Chase M C and Johnson D C. 2009. HCMV T R strain glycoprotein O acts as a chaperone promoting gH/gL incorporation into virions, but is not present in virions. J. Virol. 
         Wille P T, Knoche A J, Nelson J A, Jarvis M A and Johnson J C. 2009. An HCMV gO-null mutant fails to incorporate gH/gL into the virion envelope and is unable to enter fibroblasts, epithelial, and endothelial cells. J. Virol. 
         Shimamura M, Mach M and Britt W J. 2006. Human Cytomegalovirus infection elicits a glycoprotein M (gM)/gN-specific virus-neutralizing antibody response. J. Virol. 80:4591-4600. 
         Cha T A, Tom E, Kemble G W, Duke G M, Mocarski E S and Spaete R R. 1996. Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains. J. Virol. 70:78-83. 
         Wang D and Shenk T. 2005. Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism. Proc. Natl. Acad. Sci. USA 102:18153-58. 
         Adler B, Scrivano L, Ruzcics Z, Rupp B, Sinzger C and Kosinowski U. 2006. Role of human cytomegalovirus UL131A in cell type-specific virus entry and release. J. Gen. Virol. 87:2451-60. 
         Ryckman B J, Rainish B L, Chase M C, Borton J A, Nelson J A, Jarvis J A and Johnson D C. 2008. Characterization of the human cytomegalovirus gH/gL/UL128-UL131 complex that mediates entry into epithelial and endothelial cells. J. Virol. 82: 60-70. 
       
    
     
       
         
           
               
             
               
                   
               
               
                 SEQUENCES 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 CMV gB FL (SEQ ID NO: 25): 
               
               
                 1 - 
               
               
                 atggaaagccggatctggtgcctggtcgtgtgcgtgaacctgtgcatcgtgtgcctgggagc 
               
               
                 cgccgtgagcagcagcagcaccagaggcaccagcgccacacacagccaccacagcagccaca 
               
               
                 ccacctctgccgcccacagcagatccggcagcgtgtcccagagagtgaccagcagccagacc 
               
               
                 gtgtcccacggcgtgaacgagacaatctacaacaccaccctgaagtacggcgacgtcgtggg 
               
               
                 cgtgaataccaccaagtacccctacagagtgtgcagcatggcccagggcaccgacctgatca 
               
               
                 gattcgagcggaacatcgtgtgcaccagcatgaagcccatcaacgaggacctggacgagggc 
               
               
                 atcatggtggtgtacaagagaaacatcgtggcccacaccttcaaagtgcgggtgtaccagaa 
               
               
                 ggtgctgaccttccggcggagctacgcctacatccacaccacatacctgctgggcagcaaca 
               
               
                 ccgagtacgtggcccctcccatgtgggagatccaccacatcaacagccacagccagtgctac 
               
               
                 agcagctacagccgcgtgatcgccggcacagtgttcgtggcctaccaccgggacagctacga 
               
               
                 gaacaagaccatgcagctgatgcccgacgactacagcaacacccacagcaccagatacgtga 
               
               
                 ccgtgaaggaccagtggcacagcagaggcagcacctggctgtaccgggagacatgcaacctg 
               
               
                 aactgcatggtcaccatcaccaccgccagaagcaagtacccttaccacttcttcgccacctc 
               
               
                 caccggcgacgtggtggacatcagccccttctacaacggcaccaaccggaacgccagctact 
               
               
                 tcggcgagaacgccgacaagttcttcatcttccccaactacaccatcgtgtccgacttcggc 
               
               
                 agacccaacagcgctctggaaacccacagactggtggcctttctggaacgggccgacagcgt 
               
               
                 gatcagctgggacatccaggacgagaagaacgtgacctgccagctgaccttctgggaggcct 
               
               
                 ctgagagaaccatcagaagcgaggccgaggacagctaccacttcagcagcgccaagatgacc 
               
               
                 gccaccttcctgagcaagaaacaggaagtgaacatgagcgactccgccctggactgcgtgag 
               
               
                 ggacgaggccatcaacaagctgcagcagatcttcaacaccagctacaaccagacctacgaga 
               
               
                 agtatggcaatgtgtccgtgttcgagacaacaggcggcctggtggtgttctggcagggcatc 
               
               
                 aagcagaaaagcctggtggagctggaacggctcgccaaccggtccagcctgaacctgaccca 
               
               
                 caaccggaccaagcggagcaccgacggcaacaacgcaacccacctgtccaacatggaaagcg 
               
               
                 tgcacaacctggtgtacgcacagctgcagttcacctacgacaccctgcggggctacatcaac 
               
               
                 agagccctggcccagatcgccgaggcttggtgcgtggaccagcggcggaccctggaagtgtt 
               
               
                 caaagagctgtccaagatcaaccccagcgccatcctgagcgccatctacaacaagcctatcg 
               
               
                 ccgccagattcatgggcgacgtgctgggcctggccagctgcgtgaccatcaaccagaccagc 
               
               
                 gtgaaggtgctgcgggacatgaacgtgaaagagagcccaggccgctgctactccagacccgt 
               
               
                 ggtcatcttcaacttcgccaacagctcctacgtgcagtacggccagctgggcgaggacaacg 
               
               
                 agatcctgctggggaaccaccggaccgaggaatgccagctgcccagcctgaagatctttatc 
               
               
                 gccggcaacagcgcctacgagtatgtggactacctgttcaagcggatgatcgacctgagcag 
               
               
                 catctccaccgtggacagcatgatcgccctggacatcgaccccctggaaaacaccgacttcc 
               
               
                 gggtgctggaactgtacagccagaaagagctgcggagcagcaacgtgttcgacctggaagag 
               
               
                 atcatgcgggagttcaacagctacaagcagcgcgtgaaatacgtggaggacaaggtggtgga 
               
               
                 ccccctgcctccttacctgaagggcctggacgacctgatgagcggactgggcgctgccggaa 
               
               
                 aagccgtgggagtggccattggagctgtgggcggagctgtggcctctgtcgtggaaggcgtc 
               
               
                 gccacctttctgaagaaccccttcggcgccttcaccatcatcctggtggccattgccgtcgt 
               
               
                 gatcatcacctacctgatctacacccggcagcggagactgtgtacccagcccctgcagaacc 
               
               
                 tgttcccctacctggtgtccgccgatggcaccacagtgaccagcggctccaccaaggatacc 
               
               
                 agcctgcaggccccacccagctacgaagagagcgtgtacaacagcggcagaaagggccctgg 
               
               
                 ccctcccagctctgatgccagcacagccgcccctccctacaccaacgagcaggcctaccaga 
               
               
                 tgctgctggccctggctagactggatgccgagcagagggcccagcagaacggcaccgacagc 
               
               
                 ctggatggcagaaccggcacccaggacaagggccagaagcccaacctgctggaccggctgcg 
               
               
                 gcaccggaagaacggctaccggcacctgaaggacagcgacgaggaagagaacgtctgataa 
               
               
                 - 2727 
               
               
                   
               
               
                 CMV gB FL (SEQ ID NO: 26): 
               
               
                 MESRIWCLVVCVNLCIVCLGAAVSSSSTRGTSATHSHHSSHTTSAAHSRSGSVSQRVTSSQT 
               
               
                 VSHGVNETIYNTTLKYGDVVGVNTTKYPYRVCSMAQGTDLIRFERNIVCTSMKPINEDLDEG 
               
               
                 IMVVYKRNIVAHTFKVRVYQKVLTFRRSYAYIHTTYLLGSNTEYVAPPMWEIHHINSHSQCY 
               
               
                 SSYSRVIAGTVFVAYHRDSYENKTMQLMPDDYSNTHSTRYVTVKDQWHSRGSTWLYRETCNL 
               
               
                 NCMVTITTARSKYPYHFFATSTGDVVDISPFYNGTNRNASYFGENADKFFIFPNYTIVSDFG 
               
               
                 RPNSALETHRLVAFLERADSVISWDIQDEKNVTCQLTFWEASERTIRSEAEDSYHFSSAKMT 
               
               
                 ATFLSKKQEVNMSDSALDCVRDEAINKLQQIFNTSYNQTYEKYGNVSVFETTGGLVVFWQGI 
               
               
                 KQKSLVELERLANRSSLNLTHNRTKRSTDGNNATHLSNMESVHNLVYAQLQFTYDTLRGYIN 
               
               
                 RALAQIAEAWCVDQRRTLEVFKELSKINPSAILSAIYNKPIAARFMGDVLGLASCVTINQTS 
               
               
                 VKVLRDMNVKESPGRCYSRPVVIFNFANSSYVQYGQLGEDNEILLGNHRTEECQLPSLKIFI 
               
               
                 AGNSAYEYVDYLFKRMIDLSSISTVDSMIALDIDPLENTDFRVLELYSQKELRSSNVFDLEE 
               
               
                 IMREFNSYKQRVKYVEDKVVDPLPPYLKGLDDLMSGLGAAGKAVGVAIGAVGGAVASVVEGV 
               
               
                 ATFLKNPFGAFTIILVAIAVVIITYLIYTRQRRLCTQPLQNLFPYLVSADGTTVTSGSTKDT 
               
               
                 SLQAPPSYEESVYNSGRKGPGPPSSDASTAAPPYTNEQAYQMLLALARLDAEQRAQQNGTDS 
               
               
                 LDGRTGTQDKGQKPNLLDRLRHRKNGYRHLKDSDEEENV-- 
               
               
                   
               
               
                 CMV gB sol 750 (SEQ ID NO: 27): 
               
               
                 1- 
               
               
                 atggaaagccggatctggtgcctggtcgtgtgcgtgaacctgtgcatcgtgtgcctgggagc 
               
               
                 cgccgtgagcagcagcagcaccagaggcaccagcgccacacacagccaccacagcagccaca 
               
               
                 ccacctctgccgcccacagcagatccggcagcgtgtcccagagagtgaccagcagccagacc 
               
               
                 gtgtcccacggcgtgaacgagacaatctacaacaccaccctgaagtacggcgacgtcgtggg 
               
               
                 cgtgaataccaccaagtacccctacagagtgtgcagcatggcccagggcaccgacctgatca 
               
               
                 gattcgagcggaacatcgtgtgcaccagcatgaagcccatcaacgaggacctggacgagggc 
               
               
                 atcatggtggtgtacaagagaaacatcgtggcccacaccttcaaagtgcgggtgtaccagaa 
               
               
                 ggtgctgaccttccggcggagctacgcctacatccacaccacatacctgctgggcagcaaca 
               
               
                 ccgagtacgtggcccctcccatgtgggagatccaccacatcaacagccacagccagtgctac 
               
               
                 agcagctacagccgcgtgatcgccggcacagtgttcgtggcctaccaccgggacagctacga 
               
               
                 gaacaagaccatgcagctgatgcccgacgactacagcaacacccacagcaccagatacgtga 
               
               
                 ccgtgaaggaccagtggcacagcagaggcagcacctggctgtaccgggagacatgcaacctg 
               
               
                 aactgcatggtcaccatcaccaccgccagaagcaagtacccttaccacttcttcgccacctc 
               
               
                 caccggcgacgtggtggacatcagccccttctacaacggcaccaaccggaacgccagctact 
               
               
                 tcggcgagaacgccgacaagttcttcatcttccccaactacaccatcgtgtccgacttcggc 
               
               
                 agacccaacagcgctctggaaacccacagactggtggcctttctggaacgggccgacagcgt 
               
               
                 gatcagctgggacatccaggacgagaagaacgtgacctgccagctgaccttctgggaggcct 
               
               
                 ctgagagaaccatcagaagcgaggccgaggacagctaccacttcagcagcgccaagatgacc 
               
               
                 gccaccttcctgagcaagaaacaggaagtgaacatgagcgactccgccctggactgcgtgag 
               
               
                 ggacgaggccatcaacaagctgcagcagatcttcaacaccagctacaaccagacctacgaga 
               
               
                 agtatggcaatgtgtccgtgttcgagacaacaggcggcctggtggtgttctggcagggcatc 
               
               
                 aagcagaaaagcctggtggagctggaacggctcgccaaccggtccagcctgaacctgaccca 
               
               
                 caaccggaccaagcggagcaccgacggcaacaacgcaacccacctgtccaacatggaaagcg 
               
               
                 tgcacaacctggtgtacgcacagctgcagttcacctacgacaccctgcggggctacatcaac 
               
               
                 agagccctggcccagatcgccgaggcttggtgcgtggaccagcggcggaccctggaagtgtt 
               
               
                 caaagagctgtccaagatcaaccccagcgccatcctgagcgccatctacaacaagcctatcg 
               
               
                 ccgccagattcatgggcgacgtgctgggcctggccagctgcgtgaccatcaaccagaccagc 
               
               
                 gtgaaggtgctgcgggacatgaacgtgaaagagagcccaggccgctgctactccagacccgt 
               
               
                 ggtcatcttcaacttcgccaacagctcctacgtgcagtacggccagctgggcgaggacaacg 
               
               
                 agatcctgctggggaaccaccggaccgaggaatgccagctgcccagcctgaagatctttatc 
               
               
                 gccggcaacagcgcctacgagtatgtggactacctgttcaagcggatgatcgacctgagcag 
               
               
                 catctccaccgtggacagcatgatcgccctggacatcgaccccctggaaaacaccgacttcc 
               
               
                 gggtgctggaactgtacagccagaaagagctgcggagcagcaacgtgttcgacctggaagag 
               
               
                 atcatgcgggagttcaacagctacaagcagcgcgtgaaatacgtggaggacaaggtggtgga 
               
               
                 ccccctgcctccttacctgaagggcctggacgacctgatgagcggactgggcgctgccggaa 
               
               
                 aagccgtgggagtggccattggagctgtgggcggagctgtggcctctgtcgtggaaggcgtc 
               
               
                 gccacctttctgaagaactgataa - 2256 
               
               
                   
               
               
                 Cmv gB sol 750 (SEQ ID NO: 28): 
               
               
                 MESRIWCLVVCVNLCIVCLGAAVSSSSTRGTSATHSHHSSHTTSAAHSRSGSVSQRVTSSQT 
               
               
                 VSHGVNETIYNTTLKYGDVVGVNTTKYPYRVCSMAQGTDLIRFERNIVCTSMKPINEDLDEG 
               
               
                 IMVVYKRNIVAHTFKVRVYQKVLTFRRSYAYIHTTYLLGSNTEYVAPPMWEIHHINSHSQCY 
               
               
                 SSYSRVIAGTVFVAYHRDSYENKTMQLMPDDYSNTHSTRYVTVKDQWHSRGSTWLYRETCNL 
               
               
                 NCMVTITTARSKYPYHFFATSTGDVVDISPFYNGTNRNASYFGENADKFFIFPNYTIVSDFG 
               
               
                 RPNSALETHRLVAFLERADSVISWDIQDEKNVTCQLTFWEASERTIRSEAEDSYHFSSAKMT 
               
               
                 ATFLSKKQEVNMSDSALDCVRDEAINKLQQIFNTSYNQTYEKYGNVSVFETTGGLVVFWQGI 
               
               
                 KQKSLVELERLANRSSLNLTHNRTKRSTDGNNATHLSNMESVHNLVYAQLQFTYDTLRGYIN 
               
               
                 RALAQIAEAWCVDQRRTLEVFKELSKINPSAILSAIYNKPIAARFMGDVLGLASCVTINQTS 
               
               
                 VKVLRDMNVKESPGRCYSRPVVIFNFANSSYVQYGQLGEDNEILLGNHRTEECQLPSLKIFI 
               
               
                 AGNSAYEYVDYLFKRMIDLSSISTVDSMIALDIDPLENTDFRVLELYSQKELRSSNVFDLEE 
               
               
                 IMREFNSYKQRVKYVEDKVVDPLPPYLKGLDDLMSGLGAAGKAVGVAIGAVGGAVASVVEGV 
               
               
                 ATFLKN-- 
               
               
                   
               
               
                 CMV gB sol 692 (SEQ ID NO: 29): 
               
               
                 1- 
               
               
                 atggaaagccggatctggtgcctggtcgtgtgcgtgaacctgtgcatcgtgtgcctgggagc 
               
               
                 cgccgtgagcagcagcagcaccagaggcaccagcgccacacacagccaccacagcagccaca 
               
               
                 ccacctctgccgcccacagcagatccggcagcgtgtcccagagagtgaccagcagccagacc 
               
               
                 gtgtcccacggcgtgaacgagacaatctacaacaccaccctgaagtacggcgacgtcgtggg 
               
               
                 cgtgaataccaccaagtacccctacagagtgtgcagcatggcccagggcaccgacctgatca 
               
               
                 gattcgagcggaacatcgtgtgcaccagcatgaagcccatcaacgaggacctggacgagggc 
               
               
                 atcatggtggtgtacaagagaaacatcgtggcccacaccttcaaagtgcgggtgtaccagaa 
               
               
                 ggtgctgaccttccggcggagctacgcctacatccacaccacatacctgctgggcagcaaca 
               
               
                 ccgagtacgtggcccctcccatgtgggagatccaccacatcaacagccacagccagtgctac 
               
               
                 agcagctacagccgcgtgatcgccggcacagtgttcgtggcctaccaccgggacagctacga 
               
               
                 gaacaagaccatgcagctgatgcccgacgactacagcaacacccacagcaccagatacgtga 
               
               
                 ccgtgaaggaccagtggcacagcagaggcagcacctggctgtaccgggagacatgcaacctg 
               
               
                 aactgcatggtcaccatcaccaccgccagaagcaagtacccttaccacttcttcgccacctc 
               
               
                 caccggcgacgtggtggacatcagccccttctacaacggcaccaaccggaacgccagctact 
               
               
                 tcggcgagaacgccgacaagttcttcatcttccccaactacaccatcgtgtccgacttcggc 
               
               
                 agacccaacagcgctctggaaacccacagactggtggcctttctggaacgggccgacagcgt 
               
               
                 gatcagctgggacatccaggacgagaagaacgtgacctgccagctgaccttctgggaggcct 
               
               
                 ctgagagaaccatcagaagcgaggccgaggacagctaccacttcagcagcgccaagatgacc 
               
               
                 gccaccttcctgagcaagaaacaggaagtgaacatgagcgactccgccctggactgcgtgag 
               
               
                 ggacgaggccatcaacaagctgcagcagatcttcaacaccagctacaaccagacctacgaga 
               
               
                 agtatggcaatgtgtccgtgttcgagacaacaggcggcctggtggtgttctggcagggcatc 
               
               
                 aagcagaaaagcctggtggagctggaacggctcgccaaccggtccagcctgaacctgaccca 
               
               
                 caaccggaccaagcggagcaccgacggcaacaacgcaacccacctgtccaacatggaaagcg 
               
               
                 tgcacaacctggtgtacgcacagctgcagttcacctacgacaccctgcggggctacatcaac 
               
               
                 agagccctggcccagatcgccgaggcttggtgcgtggaccagcggcggaccctggaagtgtt 
               
               
                 caaagagctgtccaagatcaaccccagcgccatcctgagcgccatctacaacaagcctatcg 
               
               
                 ccgccagattcatgggcgacgtgctgggcctggccagctgcgtgaccatcaaccagaccagc 
               
               
                 gtgaaggtgctgcgggacatgaacgtgaaagagagcccaggccgctgctactccagacccgt 
               
               
                 ggtcatcttcaacttcgccaacagctcctacgtgcagtacggccagctgggcgaggacaacg 
               
               
                 agatcctgctggggaaccaccggaccgaggaatgccagctgcccagcctgaagatctttatc 
               
               
                 gccggcaacagcgcctacgagtatgtggactacctgttcaagcggatgatcgacctgagcag 
               
               
                 catctccaccgtggacagcatgatcgccctggacatcgaccccctggaaaacaccgacttcc 
               
               
                 gggtgctggaactgtacagccagaaagagctgcggagcagcaacgtgttcgacctggaagag 
               
               
                 atcatgcgggagttcaacagctacaagcagtgataa - 2082 
               
               
                   
               
               
                 Cmv gB sol 692 (SEQ ID NO: 30); 
               
               
                 MESRIWCLVVCVNLCIVCLGAAVSSSSTRGTSATHSHHSSHTTSAAHSRSGSVSQRVT 
               
               
                 SSQTVSHGVNETIYNTTLKYGDVVGVNTTKYPYRVCSMAQGTDLIRFERNIVCTSMK 
               
               
                 PINEDLDEGIMVVYKRNIVAHTFKVRVYQKVLTFRRSYAYIHTTYLLGSNTEYVAPP 
               
               
                 MWEIHHINSHSQCYSSYSRVIAGTVFVAYHRDSYENKTMQLMPDDYSNTHSTRYVT 
               
               
                 VKDQWHSRGSTWLYRETCNLNCMVTITTARSKYPYHFFATSTGDVVDISPFYNGTN 
               
               
                 RNASYFGENADKFFIFPNYTIVSDFGRPNSALETHRLVAFLERADSVISWDIQDEKNV 
               
               
                 TCQLTFWEASERTIRSEAEDSYHFSSAKMTATFLSKKQEVNMSDSALDCVRDEAINK 
               
               
                 LQQIFNTSYNQTYEKYGNVSVFETTGGLVVFWQGIKQKSLVELERLANRSSLNLTHN 
               
               
                 RTKRSTDGNNATHLSNMESVHNLVYAQLQFTYDTLRGYINRALAQIAEAWCVDQR 
               
               
                 RTLEVFKELSKINPSAILSAIYNKPIAARFMGDVLGLASCVTINQTSVKVLRDMNVKE 
               
               
                 SPGRCYSRPVVIFNFANSSYVQYGQLGEDNEILLGNHRTEECQLPSLKIFIAGNSAYEY 
               
               
                 VDYLFKRMIDLSSISTVDSMIALDIDPLENTDFRVLELYSQKELRSSNVFDLEEIMREF 
               
               
                 NSYKQ- 
               
               
                   
               
               
                 CMV gH FL (SEQ ID NO: 31): 
               
               
                 1- 
               
               
                 atgaggcctggcctgccctcctacctgatcatcctggccgtgtgcctgttcagccacctgctgtccagcagatac 
               
               
                 ggcgccgaggccgtgagcgagcccctggacaaggctttccacctgctgctgaacacctacggcagacccatccgg 
               
               
                 tttctgcgggagaacaccacccagtgcacctacaacagcagcctgcggaacagcaccgtcgtgagagagaacgcc 
               
               
                 atcagcttcaactttttccagagctacaaccagtactacgtgttccacatgcccagatgcctgtttgccggccct 
               
               
                 ctggccgagcagttcctgaaccaggtggacctgaccgagacactggaaagataccagcagcggctgaatacctac 
               
               
                 gccctggtgtccaaggacctggccagctaccggtcctttagccagcagctcaaggctcaggatagcctcggcgag 
               
               
                 cagcctaccaccgtgccccctcccatcgacctgagcatcccccacgtgtggatgcctccccagaccacccctcac 
               
               
                 ggctggaccgagagccacaccacctccggcctgcacagaccccacttcaaccagacctgcatcctgttcgacggc 
               
               
                 cacgacctgctgtttagcaccgtgaccccctgcctgcaccagggcttctacctgatcgacgagctgagatacgtg 
               
               
                 aagatcaccctgaccgaggatttcttcgtggtcaccgtgtccatcgacgacgacacccccatgctgctgatcttc 
               
               
                 ggccacctgcccagagtgctgttcaaggccccctaccagcgggacaacttcatcctgcggcagaccgagaagcac 
               
               
                 gagctgctggtgctggtcaagaaggaccagctgaaccggcactcctacctgaaggaccccgacttcctggacgcc 
               
               
                 gccctggacttcaactacctggacctgagcgccctgctgagaaacagcttccacagatacgccgtggacgtgctg 
               
               
                 aagtccggacggtgccagatgctcgatcggcggaccgtggagatggccttcgcctatgccctcgccctgttcgcc 
               
               
                 gctgccagacaggaagaggctggcgcccaggtgtcagtgcccagagccctggatagacaggccgccctgctgcag 
               
               
                 atccaggaattcatgatcacctgcctgagccagaccccccctagaaccaccctgctgctgtaccccacagccgtg 
               
               
                 gatctggccaagagggccctgtggacccccaaccagatcaccgacatcacaagcctcgtgcggctcgtgtacatc 
               
               
                 ctgagcaagcagaaccagcagcacctgatcccccagtgggccctgagacagatcgccgacttcgccctgaagctg 
               
               
                 cacaagacccatctggccagctttctgagcgccttcgccaggcaggaactgtacctgatgggcagcctggtccac 
               
               
                 agcatgctggtgcataccaccgagcggcgggagatcttcatcgtggagacaggcctgtgtagcctggccgagctg 
               
               
                 tcccactttacccagctgctggcccaccctcaccacgagtacctgagcgacctgtacaccccctgcagcagcagc 
               
               
                 ggcagacgggaccacagcctggaacggctgaccagactgttccccgatgccaccgtgcctgctacagtgcctgcc 
               
               
                 gccctgtccatcctgtccaccatgcagcccagcaccctggaaaccttccccgacctgttctgcctgcccctgggc 
               
               
                 gagagctttagcgccctgaccgtgtccgagcacgtgtcctacatcgtgaccaatcagtacctgatcaagggcatc 
               
               
                 agctaccccgtgtccaccacagtcgtgggccagagcctgatcatcacccagaccgacagccagaccaagtgcgag 
               
               
                 ctgacccggaacatgcacaccacacacagcatcaccgtggccctgaacatcagcctggaaaactgcgctttctgt 
               
               
                 cagtctgccctgctggaatacgacgatacccagggcgtgatcaacatcatgtacatgcacgacagcgacgacgtg 
               
               
                 ctgttcgccctggacccctacaacgaggtggtggtgtccagcccccggacccactacctgatgctgctgaagaac 
               
               
                 ggcaccgtgctggaagtgaccgacgtggtggtggacgccaccgacagcagactgctgatgatgagcgtgtacgcc 
               
               
                 ctgagcgccatcatcggcatctacctgctgtaccggatgctgaaaacctgctgataa - 2232 
               
               
                   
               
               
                 Cmv gH FL (SEQ ID NO: 32); 
               
               
                 MRPGLPSYLIILAVCLFSHLLSSRYGAEAVSEPLDKAFHLLLNTYGRPIRFLRENTTQCTYN 
               
               
                 SSLRNSTVVRENAISFNFFQSYNQYYVFHMPRCLFAGPLAEQFLNQVDLTETLERYQQRLNT 
               
               
                 YALVSKDLASYRSFSQQLKAQDSLGEQPTTVPPPIDLSIPHVWMPPQTTPHGWTESHTTSGL 
               
               
                 HRPHFNQTCILFDGHDLLFSTVTPCLHQGFYLIDELRYVKITLTEDFFVVTVSIDDDTPMLL 
               
               
                 IFGHLPRVLFKAPYQRDNFILRQTEKHELLVLVKKDQLNRHSYLKDPDFLDAALDFNYLDLS 
               
               
                 ALLRNSFHRYAVDVLKSGRCQMLDRRTVEMAFAYALALFAAARQEEAGAQVSVPRALDRQAA 
               
               
                 LLQIQEFMITCLSQTPPRTTLLLYPTAVDLAKRALWTPNQITDITSLVRLVYILSKQNQQHL 
               
               
                 IPQWALRQIADFALKLHKTHLASFLSAFARQELYLMGSLVHSMLVHTTERREIFIVETGLCS 
               
               
                 LAELSHFTQLLAHPHHEYLSDLYTPCSSSGRRDHSLERLTRLFPDATVPATVPAALSILSTM 
               
               
                 QPSTLETFPDLFCLPLGESFSALTVSEHVSYIVTNQYLIKGISYPVSTTVVGQSLIITQTDS 
               
               
                 QTKCELTRNMHTTHSITVALNISLENCAFCQSALLEYDDTQGVINIMYMHDSDDVLFALDPY 
               
               
                 NEVVVSSPRTHYLMLLKNGTVLEVTDVVVDATDSRLLMMSVYALSAIIGIYLLYRMLKTC-- 
               
               
                   
               
               
                 CMV gH sol (SEQ ID NO: 33): 
               
               
                 1- 
               
               
                 atgaggcctggcctgccctcctacctgatcatcctggccgtgtgcctgttcagccacctgct 
               
               
                 gtccagcagatacggcgccgaggccgtgagcgagcccctggacaaggctttccacctgctgc 
               
               
                 tgaacacctacggcagacccatccggtttctgcgggagaacaccacccagtgcacctacaac 
               
               
                 agcagcctgcggaacagcaccgtcgtgagagagaacgccatcagcttcaactttttccagag 
               
               
                 ctacaaccagtactacgtgttccacatgcccagatgcctgtttgccggccctctggccgagc 
               
               
                 agttcctgaaccaggtggacctgaccgagacactggaaagataccagcagcggctgaatacc 
               
               
                 tacgccctggtgtccaaggacctggccagctaccggtcctttagccagcagctcaaggctca 
               
               
                 ggatagcctcggcgagcagcctaccaccgtgccccctcccatcgacctgagcatcccccacg 
               
               
                 tgtggatgcctccccagaccacccctcacggctggaccgagagccacaccacctccggcctg 
               
               
                 cacagaccccacttcaaccagacctgcatcctgttcgacggccacgacctgctgtttagcac 
               
               
                 cgtgaccccctgcctgcaccagggcttctacctgatcgacgagctgagatacgtgaagatca 
               
               
                 ccctgaccgaggatttcttcgtggtcaccgtgtccatcgacgacgacacccccatgctgctg 
               
               
                 atcttcggccacctgcccagagtgctgttcaaggccccctaccagcgggacaacttcatcct 
               
               
                 gcggcagaccgagaagcacgagctgctggtgctggtcaagaaggaccagctgaaccggcact 
               
               
                 cctacctgaaggaccccgacttcctggacgccgccctggacttcaactacctggacctgagc 
               
               
                 gccctgctgagaaacagcttccacagatacgccgtggacgtgctgaagtccggacggtgcca 
               
               
                 gatgctcgatcggcggaccgtggagatggccttcgcctatgccctcgccctgttcgccgctg 
               
               
                 ccagacaggaagaggctggcgcccaggtgtcagtgcccagagccctggatagacaggccgcc 
               
               
                 ctgctgcagatccaggaattcatgatcacctgcctgagccagaccccccctagaaccaccct 
               
               
                 gctgctgtaccccacagccgtggatctggccaagagggccctgtggacccccaaccagatca 
               
               
                 ccgacatcacaagcctcgtgcggctcgtgtacatcctgagcaagcagaaccagcagcacctg 
               
               
                 atcccccagtgggccctgagacagatcgccgacttcgccctgaagctgcacaagacccatct 
               
               
                 ggccagctttctgagcgccttcgccaggcaggaactgtacctgatgggcagcctggtccaca 
               
               
                 gcatgctggtgcataccaccgagcggcgggagatcttcatcgtggagacaggcctgtgtagc 
               
               
                 ctggccgagctgtcccactttacccagctgctggcccaccctcaccacgagtacctgagcga 
               
               
                 cctgtacaccccctgcagcagcagcggcagacgggaccacagcctggaacggctgaccagac 
               
               
                 tgttccccgatgccaccgtgcctgctacagtgcctgccgccctgtccatcctgtccaccatg 
               
               
                 cagcccagcaccctggaaaccttccccgacctgttctgcctgcccctgggcgagagctttag 
               
               
                 cgccctgaccgtgtccgagcacgtgtcctacatcgtgaccaatcagtacctgatcaagggca 
               
               
                 tcagctaccccgtgtccaccacagtcgtgggccagagcctgatcatcacccagaccgacagc 
               
               
                 cagaccaagtgcgagctgacccggaacatgcacaccacacacagcatcaccgtggccctgaa 
               
               
                 catcagcctggaaaactgcgctttctgtcagtctgccctgctggaatacgacgatacccagg 
               
               
                 gcgtgatcaacatcatgtacatgcacgacagcgacgacgtgctgttcgccctggacccctac 
               
               
                 aacgaggtggtggtgtccagcccccggacccactacctgatgctgctgaagaacggcaccgt 
               
               
                 gctggaagtgaccgacgtggtggtggacgccaccgactgataa - 2151 
               
               
                   
               
               
                 CMV gH sol (SEQ ID NO: 34); 
               
               
                 MRPGLPSYLIILAVCLFSHLLSSRYGAEAVSEPLDKAFHLLLNTYGRPIRFLRENTTQCTYN 
               
               
                 SSLRNSTVVRENAISFNFFQSYNQYYVFHMPRCLFAGPLAEQFLNQVDLTETLERYQQRLNT 
               
               
                 YALVSKDLASYRSFSQQLKAQDSLGEQPTTVPPPIDLSIPHVWMPPQTTPHGWTESHTTSGL 
               
               
                 HRPHFNQTCILFDGHDLLFSTVTPCLHQGFYLIDELRYVKITLTEDFFVVTVSIDDDTPMLL 
               
               
                 IFGHLPRVLFKAPYQRDNFILRQTEKHELLVLVKKDQLNRHSYLKDPDFLDAALDFNYLDLS 
               
               
                 ALLRNSFHRYAVDVLKSGRCQMLDRRTVEMAFAYALALFAAARQEEAGAQVSVPRALDRQAA 
               
               
                 LLQIQEFMITCLSQTPPRTTLLLYPTAVDLAKRALWTPNQITDITSLVRLVYILSKQNQQHL 
               
               
                 IPQWALRQIADFALKLHKTHLASFLSAFARQELYLMGSLVHSMLVHTTERREIFIVETGLCS 
               
               
                 LAELSHFTQLLAHPHHEYLSDLYTPCSSSGRRDHSLERLTRLFPDATVPATVPAALSILSTM 
               
               
                 QPSTLETFPDLFCLPLGESFSALTVSEHVSYIVTNQYLIKGISYPVSTTVVGQSLIITQTDS 
               
               
                 QTKCELTRNMHTTHSITVALNISLENCAFCQSALLEYDDTQGVINIMYMHDSDDVLFALDPY 
               
               
                 NEVVVSSPRTHYLMLLKNGTVLEVTDVVVDATD-- 
               
               
                   
               
               
                 CMV gL fl (SEQ ID NO: 35): 
               
               
                 1- 
               
               
                 atgtgcagaaggcccgactgcggcttcagcttcagccctggacccgtgatcctgctgtggtg 
               
               
                 ctgcctgctgctgcctatcgtgtcctctgccgccgtgtctgtggcccctacagccgccgaga 
               
               
                 aggtgccagccgagtgccccgagctgaccagaagatgcctgctgggcgaggtgttcgagggc 
               
               
                 gacaagtacgagagctggctgcggcccctggtcaacgtgaccggcagagatggccccctgag 
               
               
                 ccagctgatccggtacagacccgtgacccccgaggccgccaatagcgtgctgctggacgagg 
               
               
                 ccttcctggataccctggccctgctgtacaacaaccccgaccagctgagagccctgctgacc 
               
               
                 ctgctgtccagcgacaccgcccccagatggatgaccgtgatgcggggctacagcgagtgtgg 
               
               
                 agatggcagccctgccgtgtacacctgcgtggacgacctgtgcagaggctacgacctgacca 
               
               
                 gactgagctacggccggtccatcttcacagagcacgtgctgggcttcgagctggtgcccccc 
               
               
                 agcctgttcaacgtggtggtggccatccggaacgaggccaccagaaccaacagagccgtgcg 
               
               
                 gctgcctgtgtctacagccgctgcacctgagggcatcacactgttctacggcctgtacaacg 
               
               
                 ccgtgaaagagttctgcctccggcaccagctggatccccccctgctgagacacctggacaag 
               
               
                 tactacgccggcctgcccccagagctgaagcagaccagagtgaacctgcccgcccacagcag 
               
               
                 atatggccctcaggccgtggacgccagatgataa - 840 
               
               
                   
               
               
                 CMV gL FL (SEQ ID NO: 36); 
               
               
                 MCRRPDCGFSFSPGPVILLWCCLLLPIVSSAAVSVAPTAAEKVPAECPELTRRCLLGEVFEG 
               
               
                 DKYESWLRPLVNVTGRDGPLSQLIRYRPVTPEAANSVLLDEAFLDTLALLYNNPDQLRALLT 
               
               
                 LLSSDTAPRWMTVMRGYSECGDGSPAVYTCVDDLCRGYDLTRLSYGRSIFTEHVLGFELVPP 
               
               
                 SLFNVVVAIRNEATRTNRAVRLPVSTAAAPEGITLFYGLYNAVKEFCLRHQLDPPLLRHLDK 
               
               
                 YYAGLPPELKQTRVNLPAHSRYGPQAVDAR-- 
               
               
                   
               
               
                 CMV gM FL (SEQ ID NO: 37): 
               
               
                 1- 
               
               
                 atggcccccagccacgtggacaaagtgaacacccggacttggagcgccagcatcgtgttcat 
               
               
                 ggtgctgaccttcgtgaacgtgtccgtgcacctggtgctgtccaacttcccccacctgggct 
               
               
                 acccctgcgtgtactaccacgtggtggacttcgagcggctgaacatgagcgcctacaacgtg 
               
               
                 atgcacctgcacacccccatgctgtttctggacagcgtgcagctcgtgtgctacgccgtgtt 
               
               
                 catgcagctggtgtttctggccgtgaccatctactacctcgtgtgctggatcaagatcagca 
               
               
                 tgcggaaggacaagggcatgagcctgaaccagagcacccgggacatcagctacatgggcgac 
               
               
                 agcctgaccgccttcctgttcatcctgagcatggacaccttccagctgttcaccctgaccat 
               
               
                 gagcttccggctgcccagcatgatcgccttcatggccgccgtgcactttttctgtctgacca 
               
               
                 tcttcaacgtgtccatggtcacccagtaccggtcctacaagcggagcctgttcttcttctcc 
               
               
                 cggctgcaccccaagctgaagggcaccgtgcagttccggaccctgatcgtgaacctggtgga 
               
               
                 ggtggccctgggcttcaataccaccgtggtggctatggccctgtgctacggcttcggcaaca 
               
               
                 acttcttcgtgcggaccggccatatggtgctggccgtgttcgtggtgtacgccatcatcagc 
               
               
                 atcatctactttctgctgatcgaggccgtgttcttccagtacgtgaaggtgcagttcggcta 
               
               
                 ccatctgggcgcctttttcggcctgtgcggcctgatctaccccatcgtgcagtacgacacct 
               
               
                 tcctgagcaacgagtaccggaccggcatcagctggtccttcggaatgctgttcttcatctgg 
               
               
                 gccatgttcaccacctgcagagccgtgcggtacttcagaggcagaggcagcggctccgtgaa 
               
               
                 gtaccaggccctggccacagcctctggcgaagaggtggccgccctgagccaccacgacagcc 
               
               
                 tggaaagcagacggctgcgggaggaagaggacgacgacgacgaggacttcgaggacgcctga 
               
               
                 taa - 1119 
               
               
                   
               
               
                 CMV gM FL (SEQ ID NO: 38); 
               
               
                 MAPSHVDKVNTRTWSASIVFMVLTFVNVSVHLVLSNFPHLGYPCVYYHVVDFERLNMSAYNV 
               
               
                 MHLHTPMLFLDSVQLVCYAVFMQLVFLAVTIYYLVCWIKISMRKDKGMSLNQSTRDISYMGD 
               
               
                 SLTAFLFILSMDTFQLFTLTMSFRLPSMIAFMAAVHFFCLTIFNVSMVTQYRSYKRSLFFFS 
               
               
                 RLHPKLKGTVQFRTLIVNLVEVALGFNTTVVAMALCYGFGNNFFVRTGHMVLAVFVVYAIIS 
               
               
                 IIYFLLIEAVFFQYVKVQFGYHLGAFFGLCGLIYPIVQYDTFLSNEYRTGISWSFGMLFFIW 
               
               
                 AMFTTCRAVRYFRGRGSGSVKYQALATASGEEVAALSHHDSLESRRLREEEDDDDEDFEDA- 
               
               
                 - 
               
               
                   
               
               
                 CMV gN FL (SEQ ID NO: 39): 
               
               
                 1- 
               
               
                 atggaatggaacaccctggtcctgggcctgctggtgctgtctgtcgtggccagcagcaacaa 
               
               
                 cacatccacagccagcacccctagacctagcagcagcacccacgccagcactaccgtgaagg 
               
               
                 ctaccaccgtggccaccacaagcaccaccactgctaccagcaccagctccaccacctctgcc 
               
               
                 aagcctggctctaccacacacgaccccaacgtgatgaggccccacgcccacaacgacttcta 
               
               
                 caacgctcactgcaccagccacatgtacgagctgtccctgagcagctttgccgcctggtgga 
               
               
                 ccatgctgaacgccctgatcctgatgggcgccttctgcatcgtgctgcggcactgctgcttc 
               
               
                 cagaacttcaccgccaccaccaccaagggctactgataa - 411 
               
               
                   
               
               
                 CMV gN FL (SEQ ID NO: 40); 
               
               
                 MEWNTLVLGLLVLSVVASSNNTSTASTPRPSSSTHASTTVKATTVATTSTTTATSTSSTTSA 
               
               
                 KPGSTTHDPNVMRPHAHNDFYNAHCTSHMYELSLSSFAAWWTMLNALILMGAFCIVLRHCCF 
               
               
                 QNFTATTTKGY-- 
               
               
                   
               
               
                 CMV gO FL (SEQ ID NO: 41): 
               
               
                 1- 
               
               
                 atgggcaagaaagaaatgatcatggtcaagggcatccccaagatcatgctgctgattagcat 
               
               
                 cacctttctgctgctgtccctgatcaactgcaacgtgctggtcaacagccggggcaccagaa 
               
               
                 gatcctggccctacaccgtgctgtcctaccggggcaaagagatcctgaagaagcagaaagag 
               
               
                 gacatcctgaagcggctgatgagcaccagcagcgacggctaccggttcctgatgtaccccag 
               
               
                 ccagcagaaattccacgccatcgtgatcagcatggacaagttcccccaggactacatcctgg 
               
               
                 ccggacccatccggaacgacagcatcacccacatgtggttcgacttctacagcacccagctg 
               
               
                 cggaagcccgccaaatacgtgtacagcgagtacaaccacaccgcccacaagatcaccctgag 
               
               
                 gcctcccccttgtggcaccgtgcccagcatgaactgcctgagcgagatgctgaacgtgtcca 
               
               
                 agcggaacgacaccggcgagaagggctgcggcaacttcaccaccttcaaccccatgttcttc 
               
               
                 aacgtgccccggtggaacaccaagctgtacatcggcagcaacaaagtgaacgtggacagcca 
               
               
                 gaccatctactttctgggcctgaccgccctgctgctgagatacgcccagcggaactgcaccc 
               
               
                 ggtccttctacctggtcaacgccatgagccggaacctgttccgggtgcccaagtacatcaac 
               
               
                 ggcaccaagctgaagaacaccatgcggaagctgaagcggaagcaggccctggtcaaagagca 
               
               
                 gccccagaagaagaacaagaagtcccagagcaccaccaccccctacctgagctacaccacct 
               
               
                 ccaccgccttcaacgtgaccaccaacgtgacctacagcgccacagccgccgtgaccagagtg 
               
               
                 gccacaagcaccaccggctaccggcccgacagcaactttatgaagtccatcatggccaccca 
               
               
                 gctgagagatctggccacctgggtgtacaccaccctgcggtacagaaacgagcccttctgca 
               
               
                 agcccgaccggaacagaaccgccgtgagcgagttcatgaagaatacccacgtgctgatcaga 
               
               
                 aacgagacaccctacaccatctacggcaccctggacatgagcagcctgtactacaacgagac 
               
               
                 aatgagcgtggagaacgagacagccagcgacaacaacgaaaccacccccacctcccccagca 
               
               
                 cccggttccagcggaccttcatcgaccccctgtgggactacctggacagcctgctgttcctg 
               
               
                 gacaagatccggaacttcagcctgcagctgcccgcctacggcaatctgaccccccctgagca 
               
               
                 cagaagggccgccaacctgagcaccctgaacagcctgtggtggtggagccagtgataa - 
               
               
                 1422 
               
               
                   
               
               
                 CMV gO FL (SEQ ID NO: 42); 
               
               
                 MGKKEMIMVKGIPKIMLLISITFLLLSLINCNVLVNSRGTRRSWPYTVLSYRGKEILKKQKE 
               
               
                 DILKRLMSTSSDGYRFLMYPSQQKFHAIVISMDKFPQDYILAGPIRNDSITHMWFDFYSTQL 
               
               
                 RKPAKYVYSEYNHTAHKITLRPPPCGTVPSMNCLSEMLNVSKRNDTGEKGCGNFTTFNPMFF 
               
               
                 NVPRWNTKLYIGSNKVNVDSQTIYFLGLTALLLRYAQRNCTRSFYLVNAMSRNLFRVPKYIN 
               
               
                 GTKLKNTMRKLKRKQALVKEQPQKKNKKSQSTTTPYLSYTTSTAFNVTTNVTYSATAAVTRV 
               
               
                 ATSTTGYRPDSNFMKSIMATQLRDLATWVYTTLRYRNEPFCKPDRNRTAVSEFMKNTHVLIR 
               
               
                 NETPYTIYGTLDMSSLYYNETMSVENETASDNNETTPTSPSTRFQRTFIDPLWDYLDSLLFL 
               
               
                 DKIRNFSLQLPAYGNLTPPEHRRAANLSTLNSLWWWSQ-- 
               
               
                   
               
               
                 CMV UL128 FL (SEQ ID NO: 43): 
               
               
                 1- 
               
               
                 atgagccccaaggacctgacccccttcctgacaaccctgtggctgctcctgggccatagcag 
               
               
                 agtgcctagagtgcgggccgaggaatgctgcgagttcatcaacgtgaaccacccccccgagc 
               
               
                 ggtgctacgacttcaagatgtgcaaccggttcaccgtggccctgagatgccccgacggcgaa 
               
               
                 gtgtgctacagccccgagaaaaccgccgagatccggggcatcgtgaccaccatgacccacag 
               
               
                 cctgacccggcaggtggtgcacaacaagctgaccagctgcaactacaaccccctgtacctgg 
               
               
                 aagccgacggccggatcagatgcggcaaagtgaacgacaaggcccagtacctgctgggagcc 
               
               
                 gccggaagcgtgccctaccggtggatcaacctggaatacgacaagatcacccggatcgtggg 
               
               
                 cctggaccagtacctggaaagcgtgaagaagcacaagcggctggacgtgtgcagagccaaga 
               
               
                 tgggctacatgctgcagtgataa - 519 
               
               
                   
               
               
                 CMV UL128 FL (SEQ ID NO: 44); 
               
               
                 MSPKDLTPFLTTLWLLLGHSRVPRVRAEECCEFINVNHPPERCYDFKMCNRFTVALRCPDGE 
               
               
                 VCYSPEKTAEIRGIVTTMTHSLTRQVVHNKLTSCNYNPLYLEADGRIRCGKVNDKAQYLLGA 
               
               
                 AGSVPYRWINLEYDKITRIVGLDQYLESVKKHKRLDVCRAKMGYMLQ-- 
               
               
                   
               
               
                 CMV UL130 FL (SEQ ID NO: 45): 
               
               
                 1- 
               
               
                 atgctgcggctgctgctgagacaccacttccactgcctgctgctgtgtgccgtgtgggccac 
               
               
                 cccttgtctggccagcccttggagcaccctgaccgccaaccagaaccctagccccccttggt 
               
               
                 ccaagctgacctacagcaagccccacgacgccgccaccttctactgcccctttctgtacccc 
               
               
                 agccctcccagaagccccctgcagttcagcggcttccagagagtgtccaccggccctgagtg 
               
               
                 ccggaacgagacactgtacctgctgtacaaccgggagggccagacactggtggagcggagca 
               
               
                 gcacctgggtgaaaaaagtgatctggtatctgagcggccggaaccagaccatcctgcagcgg 
               
               
                 atgcccagaaccgccagcaagcccagcgacggcaacgtgcagatcagcgtggaggacgccaa 
               
               
                 aatcttcggcgcccacatggtgcccaagcagaccaagctgctgagattcgtggtcaacgacg 
               
               
                 gcaccagatatcagatgtgcgtgatgaagctggaaagctgggcccacgtgttccgggactac 
               
               
                 tccgtgagcttccaggtccggctgaccttcaccgaggccaacaaccagacctacaccttctg 
               
               
                 cacccaccccaacctgatcgtgtgataa - 648 
               
               
                   
               
               
                 CMV UL130 FL (SEQ ID NO: 46); 
               
               
                 MLRLLLRHHFHCLLLCAVWATPCLASPWSTLTANQNPSPPWSKLTYSKPHDAATFYCPFLYP 
               
               
                 SPPRSPLQFSGFQRVSTGPECRNETLYLLYNREGQTLVERSSTWVKKVIWYLSGRNQTILQR 
               
               
                 MPRTASKPSDGNVQISVEDAKIFGAHMVPKQTKLLRFVVNDGTRYQMCVMKLESWAHVERDY 
               
               
                 SVSFQVRLTFTEANNQTYTFCTHPNLIV-- 
               
               
                   
               
               
                 CMV UL131 FL (SEQ ID NO: 47): 
               
               
                 1- 
               
               
                 atgcggctgtgcagagtgtggctgtccgtgtgcctgtgtgccgtggtgctgggccagtgcca 
               
               
                 gagagagacagccgagaagaacgactactaccgggtgccccactactgggatgcctgcagca 
               
               
                 gagccctgcccgaccagacccggtacaaatacgtggagcagctcgtggacctgaccctgaac 
               
               
                 taccactacgacgccagccacggcctggacaacttcgacgtgctgaagcggatcaacgtgac 
               
               
                 cgaggtgtccctgctgatcagcgacttccggcggcagaacagaagaggcggcaccaacaagc 
               
               
                 ggaccaccttcaacgccgctggctctctggcccctcacgccagatccctggaattcagcgtg 
               
               
                 cggctgttcgccaactgataa - 393 
               
               
                   
               
               
                 CMV UL131 FL (SEQ ID NO: 48); 
               
               
                 MRLCRVWLSVCLCAVVLGQCQRETAEKNDYYRVPHYWDACSRALPDQTRYKYVEQLVDLTLN 
               
               
                 YHYDASHGLDNFDVLKRINVTEVSLLISDFRRQNRRGGTNKRTTFNAAGSLAPHARSLEFSV 
               
               
                 RLFAN-- 
               
               
                   
               
               
                 EMCV IRES nucleotide sequence (SEQ ID NO: 49); 
               
               
                 aacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttc 
               
               
                 caccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacga 
               
               
                 gcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaag 
               
               
                 gaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggca 
               
               
                 gcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacac 
               
               
                 ctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaa 
               
               
                 tggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtat 
               
               
                 gggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaaac 
               
               
                 gtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgataat 
               
               
                   
               
               
                 EV71 IRES nucleotide sequence (SEQ ID NO: 50); 
               
               
                 gtacctttgtacgcctgttttataccccctccctgatttgcaacttagaagcaacgcaaacc 
               
               
                 agatcaatagtaggtgtgacataccagtcgcatcttgatcaagcacttctgtatccccggac 
               
               
                 cgagtatcaatagactgtgcacacggttgaaggagaaaacgtccgttacccggctaactact 
               
               
                 tcgagaagcctagtaacgccattgaagttgcagagtgtttcgctcagcactccccccgtgta 
               
               
                 gatcaggtcgatgagtcaccgcattccccacgggcgaccgtggcggtggctgcgttggcggc 
               
               
                 ctgcctatggggtaacccataggacgctctaatacggacatggcgtgaagagtctattgagc 
               
               
                 tagttagtagtcctccggcccctgaatgcggctaatcctaactgcggagcacatacccttaa 
               
               
                 tccaaagggcagtgtgtcgtaacgggcaactctgcagcggaaccgactactttgggtgtccg 
               
               
                 tgtttctttttattcttgtattggctgcttatggtgacaattaaagaattgttaccatatag 
               
               
                 ctattggattggccatccagtgtcaaacagagctattgtatatctctttgttggattcacac 
               
               
                 ctctcactcttgaaacgttacacaccctcaattacattatactgctgaacacgaagcg 
               
               
                   
               
               
                 VEE Subgenomic Promoter (SEQ ID NO: 51): 
               
               
                 5′-CTCTCTACGGCTAACCTGAATGGA-3′ 
               
               
                   
               
               
                 pVCR modified vector gH sol-SGP gL (SEQ ID NO: 52): 
               
               
                 cgcgtcggctacaattaatacataaccttatgtatcatacacatacgatttaggtgacacta 
               
               
                 tagatgggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcac 
               
               
                 gttgacatcgaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttga 
               
               
                 ggtagaagccaagcaggtcactgataatgaccatgctaatgccagagcgttttcgcatctgg 
               
               
                 cttcaaaactgatcgaaacggaggtggacccatccgacacgatccttgacattggaagtgcg 
               
               
                 cccgcccgcagaatgtattctaagcacaagtatcattgtatctgtccgatgagatgtgcgga 
               
               
                 agatccggacagattgtataagtatgcaactaagctgaagaaaaactgtaaggaaataactg 
               
               
                 ataaggaattggacaagaaaatgaaggagctcgccgccgtcatgagcgaccctgacctggaa 
               
               
                 actgagactatgtgcctccacgacgacgagtcgtgtcgctacgaagggcaagtcgctgttta 
               
               
                 ccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaataagggagtta 
               
               
                 gagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctggagca 
               
               
                 tatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggcct 
               
               
                 atgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatt 
               
               
                 tgaaaccatccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggac 
               
               
                 ttactgaggagctggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatg 
               
               
                 tcggtgtgagactatagttagttgcgacgggtacgtcgttaaaagaatagctatcagtccag 
               
               
                 gcctgtatgggaagccttcaggctatgctgctacgatgcaccgcgagggattcttgtgctgc 
               
               
                 aaagtgacagacacattgaacggggagagggtctcttttcccgtgtgcacgtatgtgccagc 
               
               
                 tacattgtgtgaccaaatgactggcatactggcaacagatgtcagtgcggacgacgcgcaaa 
               
               
                 aactgctggttgggctcaaccagcgtatagtcgtcaacggtcgcacccagagaaacaccaat 
               
               
                 accatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggtgggcaaaggaata 
               
               
                 taaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtcatggggt 
               
               
                 gttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaacc 
               
               
                 atcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacatt 
               
               
                 ggagatcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctc 
               
               
                 tcattaccgccgaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgt 
               
               
                 gaagccgaggagttgcgcgcagctctaccacctttggcagctgatgttgaggagcccactct 
               
               
                 ggaagccgatgtagacttgatgttacaagaggctggggccggctcagtggagacacctcgtg 
               
               
                 gcttgataaaggttaccagctacgctggcgaggacaagatcggctcttacgctgtgctttct 
               
               
                 ccgcaggctgtactcaagagtgaaaaattatcttgcatccaccctctcgctgaacaagtcat 
               
               
                 agtgataacacactctggccgaaaagggcgttatgccgtggaaccataccatggtaaagtag 
               
               
                 tggtgccagagggacatgcaatacccgtccaggactttcaagctctgagtgaaagtgccacc 
               
               
                 attgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccacacatggagg 
               
               
                 agcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcgaat 
               
               
                 acctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctc 
               
               
                 acaggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgacc 
               
               
                 agccgctccttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctg 
               
               
                 gcatcattaaaagcgcagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgt 
               
               
                 gcagaaattataagggacgtcaagaaaatgaaagggctggacgtcaatgccagaactgtgga 
               
               
                 ctcagtgctcttgaatggatgcaaacaccccgtagagaccctgtatattgacgaagcttttg 
               
               
                 cttgtcatgcaggtactctcagagcgctcatagccattataagacctaaaaaggcagtgctc 
               
               
                 tgcggggatcccaaacagtgcggtttttttaacatgatgtgcctgaaagtgcattttaacca 
               
               
                 cgagatttgcacacaagtcttccacaaaagcatctctcgccgttgcactaaatctgtgactt 
               
               
                 cggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccgaaagagactaag 
               
               
                 attgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcacttgttt 
               
               
                 cagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctg 
               
               
                 cctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcct 
               
               
                 ctgtacgcacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgt 
               
               
                 gtggaaaacactagccggcgacccatggataaaaacactgactgccaagtaccctgggaatt 
               
               
                 tcactgccacgatagaggagtggcaagcagagcatgatgccatcatgaggcacatcttggag 
               
               
                 agaccggaccctaccgacgtcttccagaataaggcaaacgtgtgttgggccaaggctttagt 
               
               
                 gccggtgctgaagaccgctggcatagacatgaccactgaacaatggaacactgtggattatt 
               
               
                 ttgaaacggacaaagctcactcagcagagatagtattgaaccaactatgcgtgaggttcttt 
               
               
                 ggactcgatctggactccggtctattttctgcacccactgttccgttatccattaggaataa 
               
               
                 tcactgggataactccccgtcgcctaacatgtacgggctgaataaagaagtggtccgtcagc 
               
               
                 tctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatgacatgaac 
               
               
                 actggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgcc 
               
               
                 tcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagca 
               
               
                 aattgaagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggtt 
               
               
                 gactggttgtcagaccggcctgaggctaccttcagagctcggctggatttaggcatcccagg 
               
               
                 tgatgtgcccaaatatgacataatatttgttaatgtgaggaccccatataaataccatcact 
               
               
                 atcagcagtgtgaagaccatgccattaagcttagcatgttgaccaagaaagcttgtctgcat 
               
               
                 ctgaatcccggcggaacctgtgtcagcataggttatggttacgctgacagggccagcgaaag 
               
               
                 catcattggtgctatagcgcggcagttcaagttttcccgggtatgcaaaccgaaatcctcac 
               
               
                 ttgaagagacggaagttctgtttgtattcattgggtacgatcgcaaggcccgtacgcacaat 
               
               
                 ccttacaagctttcatcaaccttgaccaacatttatacaggttccagactccacgaagccgg 
               
               
                 atgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaaggagtgatta 
               
               
                 taaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataagaaa 
               
               
                 ttcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgc 
               
               
                 agctaaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtg 
               
               
                 acaaacagttggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaag 
               
               
                 tcagtagcgattccactgttgtccaccggcatcttttccgggaacaaagatcgactaaccca 
               
               
                 atcattgaaccatttgctgacagctttagacaccactgatgcagatgtagccatatactgca 
               
               
                 gggacaagaaatgggaaatgactctcaaggaagcagtggctaggagagaagcagtggaggag 
               
               
                 atatgcatatccgacgactcttcagtgacagaacctgatgcagagctggtgagggtgcatcc 
               
               
                 gaagagttctttggctggaaggaagggctacagcacaagcgatggcaaaactttctcatatt 
               
               
                 tggaagggaccaagtttcaccaggcggccaaggatatagcagaaattaatgccatgtggccc 
               
               
                 gttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagcatgagcagtat 
               
               
                 taggtcgaaatgccccgtcgaagagtcggaagcctcctcaccacctagcacgctgccttgct 
               
               
                 tgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaa 
               
               
                 attactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatcca 
               
               
                 atgctcccagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatc 
               
               
                 tcgtggaaacaccaccggtagacgagactccggagccatcggcagagaaccaatccacagag 
               
               
                 gggacacctgaacaaccaccacttataaccgaggatgagaccaggactagaacgcctgagcc 
               
               
                 gatcatcatcgaagaggaagaagaggatagcataagtttgctgtcagatggcccgacccacc 
               
               
                 aggtgctgcaagtcgaggcagacattcacgggccgccctctgtatctagctcatcctggtcc 
               
               
                 attcctcatgcatccgactttgatgtggacagtttatccatacttgacaccctggagggagc 
               
               
                 tagcgtgaccagcggggcaacgtcagccgagactaactcttacttcgcaaagagtatggagt 
               
               
                 ttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctccacatcccgctccg 
               
               
                 cgcacaagaacaccgtcacttgcacccagcagggcctgctcgagagggatcacgggagaaac 
               
               
                 cgtgggatacgcggttacacacaatagcgagggcttcttgctatgcaaagttactgacacag 
               
               
                 taaaaggagaacgggtatcgttccctgtgtgcacgtacatcccggccaccataaactcgaga 
               
               
                 accagcctggtctccaacccgccaggcgtaaatagggtgattacaagagaggagtttgaggc 
               
               
                 gttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatcttttcctccgacaccg 
               
               
                 gtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtgttggag 
               
               
                 aggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtgg 
               
               
                 agaacatgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggca 
               
               
                 gaaggaaaagtggagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaa 
               
               
                 ccgtgccttttcaagccccaaggtcgcagtggaagcctgtaacgccatgttgaaagagaact 
               
               
                 ttccgactgtggcttcttactgtattattccagagtacgatgcctatttggacatggttgac 
               
               
                 ggagcttcatgctgcttagacactgccagtttttgccctgcaaagctgcgcagctttccaaa 
               
               
                 gaaacactcctatttggaacccacaatacgatcggcagtgccttcagcgatccagaacacgc 
               
               
                 tccagaacgtcctggcagctgccacaaaaagaaattgcaatgtcacgcaaatgagagaattg 
               
               
                 cccgtattggattcggcggcctttaatgtggaatgcttcaagaaatatgcgtgtaataatga 
               
               
                 atattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggtaaattaca 
               
               
                 ttaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaatatg 
               
               
                 ttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactcc 
               
               
                 aggaacaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctag 
               
               
                 caacagcgtatctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgctt 
               
               
                 ccgaacattcatacactgtttgatatgtcggctgaagactttgacgctattatagccgagca 
               
               
                 cttccagcctggggattgtgttctggaaactgacatcgcgtcgtttgataaaagtgaggacg 
               
               
                 acgccatggctctgaccgcgttaatgattctggaagacttaggtgtggacgcagagctgttg 
               
               
                 acgctgattgaggcggctttcggcgaaatttcatcaatacatttgcccactaaaactaaatt 
               
               
                 taaattcggagccatgatgaaatctggaatgttcctcacactgtttgtgaacacagtcatta 
               
               
                 acattgtaatcgcaagcagagtgttgagagaacggctaaccggatcaccatgtgcagcattc 
               
               
                 attggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcagacaggtgcgc 
               
               
                 cacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgccttatt 
               
               
                 tctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagacccc 
               
               
                 ctaaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacag 
               
               
                 gagaagggcattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgt 
               
               
                 gcaaggcagtagaatcaaggtatgaaaccgtaggaacttccatcatagttatggccatgact 
               
               
                 actctagctagcagtgttaaatcattcagctacctgagaggggcccctataactctctacgg 
               
               
                 ctaacctgaatggactacgacatagtctagtcgacgccaccatgaggcctggcctgccctcc 
               
               
                 tacctgatcatcctggccgtgtgcctgttcagccacctgctgtccagcagatacggcgccga 
               
               
                 ggccgtgagcgagcccctggacaaggctttccacctgctgctgaacacctacggcagaccca 
               
               
                 tccggtttctgcgggagaacaccacccagtgcacctacaacagcagcctgcggaacagcacc 
               
               
                 gtcgtgagagagaacgccatcagcttcaactttttccagagctacaaccagtactacgtgtt 
               
               
                 ccacatgcccagatgcctgtttgccggccctctggccgagcagttcctgaaccaggtggacc 
               
               
                 tgaccgagacactggaaagataccagcagcggctgaatacctacgccctggtgtccaaggac 
               
               
                 ctggccagctaccggtcctttagccagcagctcaaggctcaggatagcctcggcgagcagcc 
               
               
                 taccaccgtgccccctcccatcgacctgagcatcccccacgtgtggatgcctccccagacca 
               
               
                 cccctcacggctggaccgagagccacaccacctccggcctgcacagaccccacttcaaccag 
               
               
                 acctgcatcctgttcgacggccacgacctgctgtttagcaccgtgaccccctgcctgcacca 
               
               
                 gggcttctacctgatcgacgagctgagatacgtgaagatcaccctgaccgaggatttcttcg 
               
               
                 tggtcaccgtgtccatcgacgacgacacccccatgctgctgatcttcggccacctgcccaga 
               
               
                 gtgctgttcaaggccccctaccagcgggacaacttcatcctgcggcagaccgagaagcacga 
               
               
                 gctgctggtgctggtcaagaaggaccagctgaaccggcactcctacctgaaggaccccgact 
               
               
                 tcctggacgccgccctggacttcaactacctggacctgagcgccctgctgagaaacagcttc 
               
               
                 cacagatacgccgtggacgtgctgaagtccggacggtgccagatgctcgatcggcggaccgt 
               
               
                 ggagatggccttcgcctatgccctcgccctgttcgccgctgccagacaggaagaggctggcg 
               
               
                 cccaggtgtcagtgcccagagccctggatagacaggccgccctgctgcagatccaggaattc 
               
               
                 atgatcacctgcctgagccagaccccccctagaaccaccctgctgctgtaccccacagccgt 
               
               
                 ggatctggccaagagggccctgtggacccccaaccagatcaccgacatcacaagcctcgtgc 
               
               
                 ggctcgtgtacatcctgagcaagcagaaccagcagcacctgatcccccagtgggccctgaga 
               
               
                 cagatcgccgacttcgccctgaagctgcacaagacccatctggccagctttctgagcgcctt 
               
               
                 cgccaggcaggaactgtacctgatgggcagcctggtccacagcatgctggtgcataccaccg 
               
               
                 agcggcgggagatcttcatcgtggagacaggcctgtgtagcctggccgagctgtcccacttt 
               
               
                 acccagctgctggcccaccctcaccacgagtacctgagcgacctgtacaccccctgcagcag 
               
               
                 cagcggcagacgggaccacagcctggaacggctgaccagactgttccccgatgccaccgtgc 
               
               
                 ctgctacagtgcctgccgccctgtccatcctgtccaccatgcagcccagcaccctggaaacc 
               
               
                 ttccccgacctgttctgcctgcccctgggcgagagctttagcgccctgaccgtgtccgagca 
               
               
                 cgtgtcctacatcgtgaccaatcagtacctgatcaagggcatcagctaccccgtgtccacca 
               
               
                 cagtcgtgggccagagcctgatcatcacccagaccgacagccagaccaagtgcgagctgacc 
               
               
                 cggaacatgcacaccacacacagcatcaccgtggccctgaacatcagcctggaaaactgcgc 
               
               
                 tttctgtcagtctgccctgctggaatacgacgatacccagggcgtgatcaacatcatgtaca 
               
               
                 tgcacgacagcgacgacgtgctgttcgccctggacccctacaacgaggtggtggtgtccagc 
               
               
                 ccccggacccactacctgatgctgctgaagaacggcaccgtgctggaagtgaccgacgtggt 
               
               
                 ggtggacgccaccgactgataatctagacggcgcgcccacccagcggccgcctataactctc 
               
               
                 tacggctaacctgaatggactacgacatagtctagtcgacgccaccatgtgcagaaggcccg 
               
               
                 actgcggcttcagcttcagccctggacccgtgatcctgctgtggtgctgcctgctgctgcct 
               
               
                 atcgtgtcctctgccgccgtgtctgtggcccctacagccgccgagaaggtgccagccgagtg 
               
               
                 ccccgagctgaccagaagatgcctgctgggcgaggtgttcgagggcgacaagtacgagagct 
               
               
                 ggctgcggcccctggtcaacgtgaccggcagagatggccccctgagccagctgatccggtac 
               
               
                 agacccgtgacccccgaggccgccaatagcgtgctgctggacgaggccttcctggataccct 
               
               
                 ggccctgctgtacaacaaccccgaccagctgagagccctgctgaccctgctgtccagcgaca 
               
               
                 ccgcccccagatggatgaccgtgatgcggggctacagcgagtgtggagatggcagccctgcc 
               
               
                 gtgtacacctgcgtggacgacctgtgcagaggctacgacctgaccagactgagctacggccg 
               
               
                 gtccatcttcacagagcacgtgctgggcttcgagctggtgccccccagcctgttcaacgtgg 
               
               
                 tggtggccatccggaacgaggccaccagaaccaacagagccgtgcggctgcctgtgtctaca 
               
               
                 gccgctgcacctgagggcatcacactgttctacggcctgtacaacgccgtgaaagagttctg 
               
               
                 cctccggcaccagctggatccccccctgctgagacacctggacaagtactacgccggcctgc 
               
               
                 ccccagagctgaagcagaccagagtgaacctgcccgcccacagcagatatggccctcaggcc 
               
               
                 gtggacgccagatgataatctagacggcgcgcccacccaatcgatgtacttccgaggaactc 
               
               
                 acgtgcataatgcatcaggctggtacattagatccccgcttaccgcgggcaatatagcaaca 
               
               
                 ctaaaaactcgatgtacttccgaggaagcgcagtgcataatgctgcgcagtgttgccacata 
               
               
                 accactatattaaccatttatctagcggacgccaaaaactcaatgtatttctgaggaagcgt 
               
               
                 ggtgcataatgccacgcagcgtctgcataacttttattatttcttttattaatcaacaaaat 
               
               
                 tttgtttttaacatttcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaagggtc 
               
               
                 ggcatggcatctccacctcctcgcggtccgacctgggcatccgaaggaggacgcacgtccac 
               
               
                 tcggatggctaagggagagccacgagctcctgtttaaaccagctccaattcgccctatagtg 
               
               
                 agtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggc 
               
               
                 gttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaaga 
               
               
                 ggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccct 
               
               
                 gtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgcc 
               
               
                 agcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctt 
               
               
                 tccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacc 
               
               
                 tcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacg 
               
               
                 gtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactgg 
               
               
                 aacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcgg 
               
               
                 cctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatatta 
               
               
                 acgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttattt 
               
               
                 ttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaata 
               
               
                 atattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttg 
               
               
                 cggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaa 
               
               
                 gatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttga 
               
               
                 gagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcg 
               
               
                 cggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcag 
               
               
                 aatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaag 
               
               
                 agaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaa 
               
               
                 cgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgc 
               
               
                 cttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgat 
               
               
                 gcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagctt 
               
               
                 cccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcg 
               
               
                 gcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcgg 
               
               
                 tatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacgg 
               
               
                 ggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgatt 
               
               
                 aagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttca 
               
               
                 tttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatccctt 
               
               
                 aacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga 
               
               
                 gatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggt 
               
               
                 ggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagag 
               
               
                 cgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactct 
               
               
                 gtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcga 
               
               
                 taagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgg 
               
               
                 gctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgaga 
               
               
                 tacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggta 
               
               
                 tccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcct 
               
               
                 ggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgc 
               
               
                 tcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggc 
               
               
                 cttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataacc 
               
               
                 gtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgag 
               
               
                 tcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggcc 
               
               
                 gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacg 
               
               
                 caattaatgtgagttagctcactcattaggcaccccaggctttacactttatgctcccggct 
               
               
                 cgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatga 
               
               
                 ttacgccaagcgcgcaattaaccctcactaaagggaacaaaagctgggtaccggcgcca 
               
               
                   
               
               
                 pVCR modified vector gH FL-SGP gL (SEQ ID NO: 53): 
               
               
                 cgcgtcggctacaattaatacataaccttatgtatcatacacatacgatttaggtgacacta 
               
               
                 tagatgggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcac 
               
               
                 gttgacatcgaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttga 
               
               
                 ggtagaagccaagcaggtcactgataatgaccatgctaatgccagagcgttttcgcatctgg 
               
               
                 cttcaaaactgatcgaaacggaggtggacccatccgacacgatccttgacattggaagtgcg 
               
               
                 cccgcccgcagaatgtattctaagcacaagtatcattgtatctgtccgatgagatgtgcgga 
               
               
                 agatccggacagattgtataagtatgcaactaagctgaagaaaaactgtaaggaaataactg 
               
               
                 ataaggaattggacaagaaaatgaaggagctcgccgccgtcatgagcgaccctgacctggaa 
               
               
                 actgagactatgtgcctccacgacgacgagtcgtgtcgctacgaagggcaagtcgctgttta 
               
               
                 ccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaataagggagtta 
               
               
                 gagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctggagca 
               
               
                 tatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggcct 
               
               
                 atgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatt 
               
               
                 tgaaaccatccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggac 
               
               
                 ttactgaggagctggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatg 
               
               
                 tcggtgtgagactatagttagttgcgacgggtacgtcgttaaaagaatagctatcagtccag 
               
               
                 gcctgtatgggaagccttcaggctatgctgctacgatgcaccgcgagggattcttgtgctgc 
               
               
                 aaagtgacagacacattgaacggggagagggtctcttttcccgtgtgcacgtatgtgccagc 
               
               
                 tacattgtgtgaccaaatgactggcatactggcaacagatgtcagtgcggacgacgcgcaaa 
               
               
                 aactgctggttgggctcaaccagcgtatagtcgtcaacggtcgcacccagagaaacaccaat 
               
               
                 accatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggtgggcaaaggaata 
               
               
                 taaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtcatggggt 
               
               
                 gttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaacc 
               
               
                 atcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacatt 
               
               
                 ggagatcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctc 
               
               
                 tcattaccgccgaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgt 
               
               
                 gaagccgaggagttgcgcgcagctctaccacctttggcagctgatgttgaggagcccactct 
               
               
                 ggaagccgatgtagacttgatgttacaagaggctggggccggctcagtggagacacctcgtg 
               
               
                 gcttgataaaggttaccagctacgctggcgaggacaagatcggctcttacgctgtgctttct 
               
               
                 ccgcaggctgtactcaagagtgaaaaattatcttgcatccaccctctcgctgaacaagtcat 
               
               
                 agtgataacacactctggccgaaaagggcgttatgccgtggaaccataccatggtaaagtag 
               
               
                 tggtgccagagggacatgcaatacccgtccaggactttcaagctctgagtgaaagtgccacc 
               
               
                 attgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccacacatggagg 
               
               
                 agcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcgaat 
               
               
                 acctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctc 
               
               
                 acaggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgacc 
               
               
                 agccgctccttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctg 
               
               
                 gcatcattaaaagcgcagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgt 
               
               
                 gcagaaattataagggacgtcaagaaaatgaaagggctggacgtcaatgccagaactgtgga 
               
               
                 ctcagtgctcttgaatggatgcaaacaccccgtagagaccctgtatattgacgaagcttttg 
               
               
                 cttgtcatgcaggtactctcagagcgctcatagccattataagacctaaaaaggcagtgctc 
               
               
                 tgcggggatcccaaacagtgcggtttttttaacatgatgtgcctgaaagtgcattttaacca 
               
               
                 cgagatttgcacacaagtcttccacaaaagcatctctcgccgttgcactaaatctgtgactt 
               
               
                 cggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccgaaagagactaag 
               
               
                 attgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcacttgttt 
               
               
                 cagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctg 
               
               
                 cctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcct 
               
               
                 ctgtacgcacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgt 
               
               
                 gtggaaaacactagccggcgacccatggataaaaacactgactgccaagtaccctgggaatt 
               
               
                 tcactgccacgatagaggagtggcaagcagagcatgatgccatcatgaggcacatcttggag 
               
               
                 agaccggaccctaccgacgtcttccagaataaggcaaacgtgtgttgggccaaggctttagt 
               
               
                 gccggtgctgaagaccgctggcatagacatgaccactgaacaatggaacactgtggattatt 
               
               
                 ttgaaacggacaaagctcactcagcagagatagtattgaaccaactatgcgtgaggttcttt 
               
               
                 ggactcgatctggactccggtctattttctgcacccactgttccgttatccattaggaataa 
               
               
                 tcactgggataactccccgtcgcctaacatgtacgggctgaataaagaagtggtccgtcagc 
               
               
                 tctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatgacatgaac 
               
               
                 actggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgcc 
               
               
                 tcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagca 
               
               
                 aattgaagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggtt 
               
               
                 gactggttgtcagaccggcctgaggctaccttcagagctcggctggatttaggcatcccagg 
               
               
                 tgatgtgcccaaatatgacataatatttgttaatgtgaggaccccatataaataccatcact 
               
               
                 atcagcagtgtgaagaccatgccattaagcttagcatgttgaccaagaaagcttgtctgcat 
               
               
                 ctgaatcccggcggaacctgtgtcagcataggttatggttacgctgacagggccagcgaaag 
               
               
                 catcattggtgctatagcgcggcagttcaagttttcccgggtatgcaaaccgaaatcctcac 
               
               
                 ttgaagagacggaagttctgtttgtattcattgggtacgatcgcaaggcccgtacgcacaat 
               
               
                 ccttacaagctttcatcaaccttgaccaacatttatacaggttccagactccacgaagccgg 
               
               
                 atgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaaggagtgatta 
               
               
                 taaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataagaaa 
               
               
                 ttcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgc 
               
               
                 agctaaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtg 
               
               
                 acaaacagttggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaag 
               
               
                 tcagtagcgattccactgttgtccaccggcatcttttccgggaacaaagatcgactaaccca 
               
               
                 atcattgaaccatttgctgacagctttagacaccactgatgcagatgtagccatatactgca 
               
               
                 gggacaagaaatgggaaatgactctcaaggaagcagtggctaggagagaagcagtggaggag 
               
               
                 atatgcatatccgacgactcttcagtgacagaacctgatgcagagctggtgagggtgcatcc 
               
               
                 gaagagttctttggctggaaggaagggctacagcacaagcgatggcaaaactttctcatatt 
               
               
                 tggaagggaccaagtttcaccaggcggccaaggatatagcagaaattaatgccatgtggccc 
               
               
                 gttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagcatgagcagtat 
               
               
                 taggtcgaaatgccccgtcgaagagtcggaagcctcctcaccacctagcacgctgccttgct 
               
               
                 tgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaa 
               
               
                 attactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatcca 
               
               
                 atgctcccagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatc 
               
               
                 tcgtggaaacaccaccggtagacgagactccggagccatcggcagagaaccaatccacagag 
               
               
                 gggacacctgaacaaccaccacttataaccgaggatgagaccaggactagaacgcctgagcc 
               
               
                 gatcatcatcgaagaggaagaagaggatagcataagtttgctgtcagatggcccgacccacc 
               
               
                 aggtgctgcaagtcgaggcagacattcacgggccgccctctgtatctagctcatcctggtcc 
               
               
                 attcctcatgcatccgactttgatgtggacagtttatccatacttgacaccctggagggagc 
               
               
                 tagcgtgaccagcggggcaacgtcagccgagactaactcttacttcgcaaagagtatggagt 
               
               
                 ttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctccacatcccgctccg 
               
               
                 cgcacaagaacaccgtcacttgcacccagcagggcctgctcgagagggatcacgggagaaac 
               
               
                 cgtgggatacgcggttacacacaatagcgagggcttcttgctatgcaaagttactgacacag 
               
               
                 taaaaggagaacgggtatcgttccctgtgtgcacgtacatcccggccaccataaactcgaga 
               
               
                 accagcctggtctccaacccgccaggcgtaaatagggtgattacaagagaggagtttgaggc 
               
               
                 gttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatcttttcctccgacaccg 
               
               
                 gtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtgttggag 
               
               
                 aggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtgg 
               
               
                 agaacatgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggca 
               
               
                 gaaggaaaagtggagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaa 
               
               
                 ccgtgccttttcaagccccaaggtcgcagtggaagcctgtaacgccatgttgaaagagaact 
               
               
                 ttccgactgtggcttcttactgtattattccagagtacgatgcctatttggacatggttgac 
               
               
                 ggagcttcatgctgcttagacactgccagtttttgccctgcaaagctgcgcagctttccaaa 
               
               
                 gaaacactcctatttggaacccacaatacgatcggcagtgccttcagcgatccagaacacgc 
               
               
                 tccagaacgtcctggcagctgccacaaaaagaaattgcaatgtcacgcaaatgagagaattg 
               
               
                 cccgtattggattcggcggcctttaatgtggaatgcttcaagaaatatgcgtgtaataatga 
               
               
                 atattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggtaaattaca 
               
               
                 ttaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaatatg 
               
               
                 ttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactcc 
               
               
                 aggaacaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctag 
               
               
                 caacagcgtatctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgctt 
               
               
                 ccgaacattcatacactgtttgatatgtcggctgaagactttgacgctattatagccgagca 
               
               
                 cttccagcctggggattgtgttctggaaactgacatcgcgtcgtttgataaaagtgaggacg 
               
               
                 acgccatggctctgaccgcgttaatgattctggaagacttaggtgtggacgcagagctgttg 
               
               
                 acgctgattgaggcggctttcggcgaaatttcatcaatacatttgcccactaaaactaaatt 
               
               
                 taaattcggagccatgatgaaatctggaatgttcctcacactgtttgtgaacacagtcatta 
               
               
                 acattgtaatcgcaagcagagtgttgagagaacggctaaccggatcaccatgtgcagcattc 
               
               
                 attggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcagacaggtgcgc 
               
               
                 cacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgccttatt 
               
               
                 tctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagacccc 
               
               
                 ctaaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacag 
               
               
                 gagaagggcattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgt 
               
               
                 gcaaggcagtagaatcaaggtatgaaaccgtaggaacttccatcatagttatggccatgact 
               
               
                 actctagctagcagtgttaaatcattcagctacctgagaggggcccctataactctctacgg 
               
               
                 ctaacctgaatggactacgacatagtctagtcgacgccaccatgaggcctggcctgccctcc 
               
               
                 tacctgatcatcctggccgtgtgcctgttcagccacctgctgtccagcagatacggcgccga 
               
               
                 ggccgtgagcgagcccctggacaaggctttccacctgctgctgaacacctacggcagaccca 
               
               
                 tccggtttctgcgggagaacaccacccagtgcacctacaacagcagcctgcggaacagcacc 
               
               
                 gtcgtgagagagaacgccatcagcttcaactttttccagagctacaaccagtactacgtgtt 
               
               
                 ccacatgcccagatgcctgtttgccggccctctggccgagcagttcctgaaccaggtggacc 
               
               
                 tgaccgagacactggaaagataccagcagcggctgaatacctacgccctggtgtccaaggac 
               
               
                 ctggccagctaccggtcctttagccagcagctcaaggctcaggatagcctcggcgagcagcc 
               
               
                 taccaccgtgccccctcccatcgacctgagcatcccccacgtgtggatgcctccccagacca 
               
               
                 cccctcacggctggaccgagagccacaccacctccggcctgcacagaccccacttcaaccag 
               
               
                 acctgcatcctgttcgacggccacgacctgctgtttagcaccgtgaccccctgcctgcacca 
               
               
                 gggcttctacctgatcgacgagctgagatacgtgaagatcaccctgaccgaggatttcttcg 
               
               
                 tggtcaccgtgtccatcgacgacgacacccccatgctgctgatcttcggccacctgcccaga 
               
               
                 gtgctgttcaaggccccctaccagcgggacaacttcatcctgcggcagaccgagaagcacga 
               
               
                 gctgctggtgctggtcaagaaggaccagctgaaccggcactcctacctgaaggaccccgact 
               
               
                 tcctggacgccgccctggacttcaactacctggacctgagcgccctgctgagaaacagcttc 
               
               
                 cacagatacgccgtggacgtgctgaagtccggacggtgccagatgctcgatcggcggaccgt 
               
               
                 ggagatggccttcgcctatgccctcgccctgttcgccgctgccagacaggaagaggctggcg 
               
               
                 cccaggtgtcagtgcccagagccctggatagacaggccgccctgctgcagatccaggaattc 
               
               
                 atgatcacctgcctgagccagaccccccctagaaccaccctgctgctgtaccccacagccgt 
               
               
                 ggatctggccaagagggccctgtggacccccaaccagatcaccgacatcacaagcctcgtgc 
               
               
                 ggctcgtgtacatcctgagcaagcagaaccagcagcacctgatcccccagtgggccctgaga 
               
               
                 cagatcgccgacttcgccctgaagctgcacaagacccatctggccagctttctgagcgcctt 
               
               
                 cgccaggcaggaactgtacctgatgggcagcctggtccacagcatgctggtgcataccaccg 
               
               
                 agcggcgggagatcttcatcgtggagacaggcctgtgtagcctggccgagctgtcccacttt 
               
               
                 acccagctgctggcccaccctcaccacgagtacctgagcgacctgtacaccccctgcagcag 
               
               
                 cagcggcagacgggaccacagcctggaacggctgaccagactgttccccgatgccaccgtgc 
               
               
                 ctgctacagtgcctgccgccctgtccatcctgtccaccatgcagcccagcaccctggaaacc 
               
               
                 ttccccgacctgttctgcctgcccctgggcgagagctttagcgccctgaccgtgtccgagca 
               
               
                 cgtgtcctacatcgtgaccaatcagtacctgatcaagggcatcagctaccccgtgtccacca 
               
               
                 cagtcgtgggccagagcctgatcatcacccagaccgacagccagaccaagtgcgagctgacc 
               
               
                 cggaacatgcacaccacacacagcatcaccgtggccctgaacatcagcctggaaaactgcgc 
               
               
                 tttctgtcagtctgccctgctggaatacgacgatacccagggcgtgatcaacatcatgtaca 
               
               
                 tgcacgacagcgacgacgtgctgttcgccctggacccctacaacgaggtggtggtgtccagc 
               
               
                 ccccggacccactacctgatgctgctgaagaacggcaccgtgctggaagtgaccgacgtggt 
               
               
                 ggtggacgccaccgacagcagactgctgatgatgagcgtgtacgccctgagcgccatcatcg 
               
               
                 gcatctacctgctgtaccggatgctgaaaacctgctgataatctagacggcgcgcccaccca 
               
               
                 gcggccgcctataactctctacggctaacctgaatggactacgacatagtctagtcgacgcc 
               
               
                 accatgtgcagaaggcccgactgcggcttcagcttcagccctggacccgtgatcctgctgtg 
               
               
                 gtgctgcctgctgctgcctatcgtgtcctctgccgccgtgtctgtggcccctacagccgccg 
               
               
                 agaaggtgccagccgagtgccccgagctgaccagaagatgcctgctgggcgaggtgttcgag 
               
               
                 ggcgacaagtacgagagctggctgcggcccctggtcaacgtgaccggcagagatggccccct 
               
               
                 gagccagctgatccggtacagacccgtgacccccgaggccgccaatagcgtgctgctggacg 
               
               
                 aggccttcctggataccctggccctgctgtacaacaaccccgaccagctgagagccctgctg 
               
               
                 accctgctgtccagcgacaccgcccccagatggatgaccgtgatgcggggctacagcgagtg 
               
               
                 tggagatggcagccctgccgtgtacacctgcgtggacgacctgtgcagaggctacgacctga 
               
               
                 ccagactgagctacggccggtccatcttcacagagcacgtgctgggcttcgagctggtgccc 
               
               
                 cccagcctgttcaacgtggtggtggccatccggaacgaggccaccagaaccaacagagccgt 
               
               
                 gcggctgcctgtgtctacagccgctgcacctgagggcatcacactgttctacggcctgtaca 
               
               
                 acgccgtgaaagagttctgcctccggcaccagctggatccccccctgctgagacacctggac 
               
               
                 aagtactacgccggcctgcccccagagctgaagcagaccagagtgaacctgcccgcccacag 
               
               
                 cagatatggccctcaggccgtggacgccagatgataatctagacggcgcgcccacccaatcg 
               
               
                 atgtacttccgaggaactcacgtgcataatgcatcaggctggtacattagatccccgcttac 
               
               
                 cgcgggcaatatagcaacactaaaaactcgatgtacttccgaggaagcgcagtgcataatgc 
               
               
                 tgcgcagtgttgccacataaccactatattaaccatttatctagcggacgccaaaaactcaa 
               
               
                 tgtatttctgaggaagcgtggtgcataatgccacgcagcgtctgcataacttttattatttc 
               
               
                 ttttattaatcaacaaaattttgtttttaacatttcaaaaaaaaaaaaaaaaaaaaaaaaaa 
               
               
                 aaaaaaaaaaaaaagggtcggcatggcatctccacctcctcgcggtccgacctgggcatccg 
               
               
                 aaggaggacgcacgtccactcggatggctaagggagagccacgagctcctgtttaaaccagc 
               
               
                 tccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcg 
               
               
                 tgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgcca 
               
               
                 gctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaat 
               
               
                 ggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcag 
               
               
                 cgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttc 
               
               
                 tcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccga 
               
               
                 tttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgg 
               
               
                 gccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtg 
               
               
                 gactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataa 
               
               
                 gggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgc 
               
               
                 gaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcgg 
               
               
                 aacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataac 
               
               
                 cctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtc 
               
               
                 gcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggt 
               
               
                 gaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctca 
               
               
                 acagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactttt 
               
               
                 aaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcg 
               
               
                 ccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatctta 
               
               
                 cggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcg 
               
               
                 gccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacat 
               
               
                 gggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacg 
               
               
                 acgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggc 
               
               
                 gaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgc 
               
               
                 aggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccg 
               
               
                 gtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatc 
               
               
                 gtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctga 
               
               
                 gataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatacttt 
               
               
                 agattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataat 
               
               
                 ctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaa 
               
               
                 gatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaa 
               
               
                 aaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaag 
               
               
                 gtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttagg 
               
               
                 ccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccag 
               
               
                 tggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg 
               
               
                 gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaac 
               
               
                 gacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaag 
               
               
                 ggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggag 
               
               
                 cttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttga 
               
               
                 gcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcgg 
               
               
                 cctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcc 
               
               
                 cctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccg 
               
               
                 aacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgc 
               
               
                 ctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaa 
               
               
                 gcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggcttt 
               
               
                 acactttatgctcccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacag 
               
               
                 gaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaag 
               
               
                 ctgggtaccggcgcca 
               
               
                   
               
               
                 pVCR modified vector gH sol-SGP gL-SGP gO (SEQ ID NO: 54): 
               
               
                 cgcgtcggctacaattaatacataaccttatgtatcatacacatacgatttaggtgacacta 
               
               
                 tagatgggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcac 
               
               
                 gttgacatcgaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttga 
               
               
                 ggtagaagccaagcaggtcactgataatgaccatgctaatgccagagcgttttcgcatctgg 
               
               
                 cttcaaaactgatcgaaacggaggtggacccatccgacacgatccttgacattggaagtgcg 
               
               
                 cccgcccgcagaatgtattctaagcacaagtatcattgtatctgtccgatgagatgtgcgga 
               
               
                 agatccggacagattgtataagtatgcaactaagctgaagaaaaactgtaaggaaataactg 
               
               
                 ataaggaattggacaagaaaatgaaggagctcgccgccgtcatgagcgaccctgacctggaa 
               
               
                 actgagactatgtgcctccacgacgacgagtcgtgtcgctacgaagggcaagtcgctgttta 
               
               
                 ccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaataagggagtta 
               
               
                 gagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctggagca 
               
               
                 tatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggcct 
               
               
                 atgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatt 
               
               
                 tgaaaccatccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggac 
               
               
                 ttactgaggagctggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatg 
               
               
                 tcggtgtgagactatagttagttgcgacgggtacgtcgttaaaagaatagctatcagtccag 
               
               
                 gcctgtatgggaagccttcaggctatgctgctacgatgcaccgcgagggattcttgtgctgc 
               
               
                 aaagtgacagacacattgaacggggagagggtctcttttcccgtgtgcacgtatgtgccagc 
               
               
                 tacattgtgtgaccaaatgactggcatactggcaacagatgtcagtgcggacgacgcgcaaa 
               
               
                 aactgctggttgggctcaaccagcgtatagtcgtcaacggtcgcacccagagaaacaccaat 
               
               
                 accatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggtgggcaaaggaata 
               
               
                 taaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtcatggggt 
               
               
                 gttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaacc 
               
               
                 atcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacatt 
               
               
                 ggagatcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctc 
               
               
                 tcattaccgccgaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgt 
               
               
                 gaagccgaggagttgcgcgcagctctaccacctttggcagctgatgttgaggagcccactct 
               
               
                 ggaagccgatgtagacttgatgttacaagaggctggggccggctcagtggagacacctcgtg 
               
               
                 gcttgataaaggttaccagctacgctggcgaggacaagatcggctcttacgctgtgctttct 
               
               
                 ccgcaggctgtactcaagagtgaaaaattatcttgcatccaccctctcgctgaacaagtcat 
               
               
                 agtgataacacactctggccgaaaagggcgttatgccgtggaaccataccatggtaaagtag 
               
               
                 tggtgccagagggacatgcaatacccgtccaggactttcaagctctgagtgaaagtgccacc 
               
               
                 attgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccacacatggagg 
               
               
                 agcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcgaat 
               
               
                 acctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctc 
               
               
                 acaggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgacc 
               
               
                 agccgctccttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctg 
               
               
                 gcatcattaaaagcgcagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgt 
               
               
                 gcagaaattataagggacgtcaagaaaatgaaagggctggacgtcaatgccagaactgtgga 
               
               
                 ctcagtgctcttgaatggatgcaaacaccccgtagagaccctgtatattgacgaagcttttg 
               
               
                 cttgtcatgcaggtactctcagagcgctcatagccattataagacctaaaaaggcagtgctc 
               
               
                 tgcggggatcccaaacagtgcggtttttttaacatgatgtgcctgaaagtgcattttaacca 
               
               
                 cgagatttgcacacaagtcttccacaaaagcatctctcgccgttgcactaaatctgtgactt 
               
               
                 cggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccgaaagagactaag 
               
               
                 attgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcacttgttt 
               
               
                 cagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctg 
               
               
                 cctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcct 
               
               
                 ctgtacgcacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgt 
               
               
                 gtggaaaacactagccggcgacccatggataaaaacactgactgccaagtaccctgggaatt 
               
               
                 tcactgccacgatagaggagtggcaagcagagcatgatgccatcatgaggcacatcttggag 
               
               
                 agaccggaccctaccgacgtcttccagaataaggcaaacgtgtgttgggccaaggctttagt 
               
               
                 gccggtgctgaagaccgctggcatagacatgaccactgaacaatggaacactgtggattatt 
               
               
                 ttgaaacggacaaagctcactcagcagagatagtattgaaccaactatgcgtgaggttcttt 
               
               
                 ggactcgatctggactccggtctattttctgcacccactgttccgttatccattaggaataa 
               
               
                 tcactgggataactccccgtcgcctaacatgtacgggctgaataaagaagtggtccgtcagc 
               
               
                 tctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatgacatgaac 
               
               
                 actggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgcc 
               
               
                 tcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagca 
               
               
                 aattgaagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggtt 
               
               
                 gactggttgtcagaccggcctgaggctaccttcagagctcggctggatttaggcatcccagg 
               
               
                 tgatgtgcccaaatatgacataatatttgttaatgtgaggaccccatataaataccatcact 
               
               
                 atcagcagtgtgaagaccatgccattaagcttagcatgttgaccaagaaagcttgtctgcat 
               
               
                 ctgaatcccggcggaacctgtgtcagcataggttatggttacgctgacagggccagcgaaag 
               
               
                 catcattggtgctatagcgcggcagttcaagttttcccgggtatgcaaaccgaaatcctcac 
               
               
                 ttgaagagacggaagttctgtttgtattcattgggtacgatcgcaaggcccgtacgcacaat 
               
               
                 ccttacaagctttcatcaaccttgaccaacatttatacaggttccagactccacgaagccgg 
               
               
                 atgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaaggagtgatta 
               
               
                 taaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataagaaa 
               
               
                 ttcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgc 
               
               
                 agctaaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtg 
               
               
                 acaaacagttggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaag 
               
               
                 tcagtagcgattccactgttgtccaccggcatcttttccgggaacaaagatcgactaaccca 
               
               
                 atcattgaaccatttgctgacagctttagacaccactgatgcagatgtagccatatactgca 
               
               
                 gggacaagaaatgggaaatgactctcaaggaagcagtggctaggagagaagcagtggaggag 
               
               
                 atatgcatatccgacgactcttcagtgacagaacctgatgcagagctggtgagggtgcatcc 
               
               
                 gaagagttctttggctggaaggaagggctacagcacaagcgatggcaaaactttctcatatt 
               
               
                 tggaagggaccaagtttcaccaggcggccaaggatatagcagaaattaatgccatgtggccc 
               
               
                 gttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagcatgagcagtat 
               
               
                 taggtcgaaatgccccgtcgaagagtcggaagcctcctcaccacctagcacgctgccttgct 
               
               
                 tgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaa 
               
               
                 attactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatcca 
               
               
                 atgctcccagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatc 
               
               
                 tcgtggaaacaccaccggtagacgagactccggagccatcggcagagaaccaatccacagag 
               
               
                 gggacacctgaacaaccaccacttataaccgaggatgagaccaggactagaacgcctgagcc 
               
               
                 gatcatcatcgaagaggaagaagaggatagcataagtttgctgtcagatggcccgacccacc 
               
               
                 aggtgctgcaagtcgaggcagacattcacgggccgccctctgtatctagctcatcctggtcc 
               
               
                 attcctcatgcatccgactttgatgtggacagtttatccatacttgacaccctggagggagc 
               
               
                 tagcgtgaccagcggggcaacgtcagccgagactaactcttacttcgcaaagagtatggagt 
               
               
                 ttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctccacatcccgctccg 
               
               
                 cgcacaagaacaccgtcacttgcacccagcagggcctgctcgagagggatcacgggagaaac 
               
               
                 cgtgggatacgcggttacacacaatagcgagggcttcttgctatgcaaagttactgacacag 
               
               
                 taaaaggagaacgggtatcgttccctgtgtgcacgtacatcccggccaccataaactcgaga 
               
               
                 accagcctggtctccaacccgccaggcgtaaatagggtgattacaagagaggagtttgaggc 
               
               
                 gttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatcttttcctccgacaccg 
               
               
                 gtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtgttggag 
               
               
                 aggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtgg 
               
               
                 agaacatgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggca 
               
               
                 gaaggaaaagtggagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaa 
               
               
                 ccgtgccttttcaagccccaaggtcgcagtggaagcctgtaacgccatgttgaaagagaact 
               
               
                 ttccgactgtggcttcttactgtattattccagagtacgatgcctatttggacatggttgac 
               
               
                 ggagcttcatgctgcttagacactgccagtttttgccctgcaaagctgcgcagctttccaaa 
               
               
                 gaaacactcctatttggaacccacaatacgatcggcagtgccttcagcgatccagaacacgc 
               
               
                 tccagaacgtcctggcagctgccacaaaaagaaattgcaatgtcacgcaaatgagagaattg 
               
               
                 cccgtattggattcggcggcctttaatgtggaatgcttcaagaaatatgcgtgtaataatga 
               
               
                 atattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggtaaattaca 
               
               
                 ttaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaatatg 
               
               
                 ttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactcc 
               
               
                 aggaacaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctag 
               
               
                 caacagcgtatctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgctt 
               
               
                 ccgaacattcatacactgtttgatatgtcggctgaagactttgacgctattatagccgagca 
               
               
                 cttccagcctggggattgtgttctggaaactgacatcgcgtcgtttgataaaagtgaggacg 
               
               
                 acgccatggctctgaccgcgttaatgattctggaagacttaggtgtggacgcagagctgttg 
               
               
                 acgctgattgaggcggctttcggcgaaatttcatcaatacatttgcccactaaaactaaatt 
               
               
                 taaattcggagccatgatgaaatctggaatgttcctcacactgtttgtgaacacagtcatta 
               
               
                 acattgtaatcgcaagcagagtgttgagagaacggctaaccggatcaccatgtgcagcattc 
               
               
                 attggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcagacaggtgcgc 
               
               
                 cacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgccttatt 
               
               
                 tctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagacccc 
               
               
                 ctaaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacag 
               
               
                 gagaagggcattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgt 
               
               
                 gcaaggcagtagaatcaaggtatgaaaccgtaggaacttccatcatagttatggccatgact 
               
               
                 actctagctagcagtgttaaatcattcagctacctgagaggggcccctataactctctacgg 
               
               
                 ctaacctgaatggactacgacatagtctagtcgacgccaccatgaggcctggcctgccctcc 
               
               
                 tacctgatcatcctggccgtgtgcctgttcagccacctgctgtccagcagatacggcgccga 
               
               
                 ggccgtgagcgagcccctggacaaggctttccacctgctgctgaacacctacggcagaccca 
               
               
                 tccggtttctgcgggagaacaccacccagtgcacctacaacagcagcctgcggaacagcacc 
               
               
                 gtcgtgagagagaacgccatcagcttcaactttttccagagctacaaccagtactacgtgtt 
               
               
                 ccacatgcccagatgcctgtttgccggccctctggccgagcagttcctgaaccaggtggacc 
               
               
                 tgaccgagacactggaaagataccagcagcggctgaatacctacgccctggtgtccaaggac 
               
               
                 ctggccagctaccggtcctttagccagcagctcaaggctcaggatagcctcggcgagcagcc 
               
               
                 taccaccgtgccccctcccatcgacctgagcatcccccacgtgtggatgcctccccagacca 
               
               
                 cccctcacggctggaccgagagccacaccacctccggcctgcacagaccccacttcaaccag 
               
               
                 acctgcatcctgttcgacggccacgacctgctgtttagcaccgtgaccccctgcctgcacca 
               
               
                 gggcttctacctgatcgacgagctgagatacgtgaagatcaccctgaccgaggatttcttcg 
               
               
                 tggtcaccgtgtccatcgacgacgacacccccatgctgctgatcttcggccacctgcccaga 
               
               
                 gtgctgttcaaggccccctaccagcgggacaacttcatcctgcggcagaccgagaagcacga 
               
               
                 gctgctggtgctggtcaagaaggaccagctgaaccggcactcctacctgaaggaccccgact 
               
               
                 tcctggacgccgccctggacttcaactacctggacctgagcgccctgctgagaaacagcttc 
               
               
                 cacagatacgccgtggacgtgctgaagtccggacggtgccagatgctcgatcggcggaccgt 
               
               
                 ggagatggccttcgcctatgccctcgccctgttcgccgctgccagacaggaagaggctggcg 
               
               
                 cccaggtgtcagtgcccagagccctggatagacaggccgccctgctgcagatccaggaattc 
               
               
                 atgatcacctgcctgagccagaccccccctagaaccaccctgctgctgtaccccacagccgt 
               
               
                 ggatctggccaagagggccctgtggacccccaaccagatcaccgacatcacaagcctcgtgc 
               
               
                 ggctcgtgtacatcctgagcaagcagaaccagcagcacctgatcccccagtgggccctgaga 
               
               
                 cagatcgccgacttcgccctgaagctgcacaagacccatctggccagctttctgagcgcctt 
               
               
                 cgccaggcaggaactgtacctgatgggcagcctggtccacagcatgctggtgcataccaccg 
               
               
                 agcggcgggagatcttcatcgtggagacaggcctgtgtagcctggccgagctgtcccacttt 
               
               
                 acccagctgctggcccaccctcaccacgagtacctgagcgacctgtacaccccctgcagcag 
               
               
                 cagcggcagacgggaccacagcctggaacggctgaccagactgttccccgatgccaccgtgc 
               
               
                 ctgctacagtgcctgccgccctgtccatcctgtccaccatgcagcccagcaccctggaaacc 
               
               
                 ttccccgacctgttctgcctgcccctgggcgagagctttagcgccctgaccgtgtccgagca 
               
               
                 cgtgtcctacatcgtgaccaatcagtacctgatcaagggcatcagctaccccgtgtccacca 
               
               
                 cagtcgtgggccagagcctgatcatcacccagaccgacagccagaccaagtgcgagctgacc 
               
               
                 cggaacatgcacaccacacacagcatcaccgtggccctgaacatcagcctggaaaactgcgc 
               
               
                 tttctgtcagtctgccctgctggaatacgacgatacccagggcgtgatcaacatcatgtaca 
               
               
                 tgcacgacagcgacgacgtgctgttcgccctggacccctacaacgaggtggtggtgtccagc 
               
               
                 ccccggacccactacctgatgctgctgaagaacggcaccgtgctggaagtgaccgacgtggt 
               
               
                 ggtggacgccaccgactgataatctagacggcgcgcccacccagcggccgcctataactctc 
               
               
                 tacggctaacctgaatggactacgacatagtctagtcgacgccaccatgtgcagaaggcccg 
               
               
                 actgcggcttcagcttcagccctggacccgtgatcctgctgtggtgctgcctgctgctgcct 
               
               
                 atcgtgtcctctgccgccgtgtctgtggcccctacagccgccgagaaggtgccagccgagtg 
               
               
                 ccccgagctgaccagaagatgcctgctgggcgaggtgttcgagggcgacaagtacgagagct 
               
               
                 ggctgcggcccctggtcaacgtgaccggcagagatggccccctgagccagctgatccggtac 
               
               
                 agacccgtgacccccgaggccgccaatagcgtgctgctggacgaggccttcctggataccct 
               
               
                 ggccctgctgtacaacaaccccgaccagctgagagccctgctgaccctgctgtccagcgaca 
               
               
                 ccgcccccagatggatgaccgtgatgcggggctacagcgagtgtggagatggcagccctgcc 
               
               
                 gtgtacacctgcgtggacgacctgtgcagaggctacgacctgaccagactgagctacggccg 
               
               
                 gtccatcttcacagagcacgtgctgggcttcgagctggtgccccccagcctgttcaacgtgg 
               
               
                 tggtggccatccggaacgaggccaccagaaccaacagagccgtgcggctgcctgtgtctaca 
               
               
                 gccgctgcacctgagggcatcacactgttctacggcctgtacaacgccgtgaaagagttctg 
               
               
                 cctccggcaccagctggatccccccctgctgagacacctggacaagtactacgccggcctgc 
               
               
                 ccccagagctgaagcagaccagagtgaacctgcccgcccacagcagatatggccctcaggcc 
               
               
                 gtggacgccagatgataatctagacggcgcgcccacccaatcgatctataactctctacggc 
               
               
                 taacctgaatggactacgacatagtctagtcgacgccaccatgggcaagaaagaaatgatca 
               
               
                 tggtcaagggcatccccaagatcatgctgctgattagcatcacctttctgctgctgtccctg 
               
               
                 atcaactgcaacgtgctggtcaacagccggggcaccagaagatcctggccctacaccgtgct 
               
               
                 gtcctaccggggcaaagagatcctgaagaagcagaaagaggacatcctgaagcggctgatga 
               
               
                 gcaccagcagcgacggctaccggttcctgatgtaccccagccagcagaaattccacgccatc 
               
               
                 gtgatcagcatggacaagttcccccaggactacatcctggccggacccatccggaacgacag 
               
               
                 catcacccacatgtggttcgacttctacagcacccagctgcggaagcccgccaaatacgtgt 
               
               
                 acagcgagtacaaccacaccgcccacaagatcaccctgaggcctcccccttgtggcaccgtg 
               
               
                 cccagcatgaactgcctgagcgagatgctgaacgtgtccaagcggaacgacaccggcgagaa 
               
               
                 gggctgcggcaacttcaccaccttcaaccccatgttcttcaacgtgccccggtggaacacca 
               
               
                 agctgtacatcggcagcaacaaagtgaacgtggacagccagaccatctactttctgggcctg 
               
               
                 accgccctgctgctgagatacgcccagcggaactgcacccggtccttctacctggtcaacgc 
               
               
                 catgagccggaacctgttccgggtgcccaagtacatcaacggcaccaagctgaagaacacca 
               
               
                 tgcggaagctgaagcggaagcaggccctggtcaaagagcagccccagaagaagaacaagaag 
               
               
                 tcccagagcaccaccaccccctacctgagctacaccacctccaccgccttcaacgtgaccac 
               
               
                 caacgtgacctacagcgccacagccgccgtgaccagagtggccacaagcaccaccggctacc 
               
               
                 ggcccgacagcaactttatgaagtccatcatggccacccagctgagagatctggccacctgg 
               
               
                 gtgtacaccaccctgcggtacagaaacgagcccttctgcaagcccgaccggaacagaaccgc 
               
               
                 cgtgagcgagttcatgaagaatacccacgtgctgatcagaaacgagacaccctacaccatct 
               
               
                 acggcaccctggacatgagcagcctgtactacaacgagacaatgagcgtggagaacgagaca 
               
               
                 gccagcgacaacaacgaaaccacccccacctcccccagcacccggttccagcggaccttcat 
               
               
                 cgaccccctgtgggactacctggacagcctgctgttcctggacaagatccggaacttcagcc 
               
               
                 tgcagctgcccgcctacggcaatctgaccccccctgagcacagaagggccgccaacctgagc 
               
               
                 accctgaacagcctgtggtggtggagccagtgataatctagacggcgcgcccacccaccgcg 
               
               
                 ggcaatatagcaacactaaaaactcgatgtacttccgaggaagcgcagtgcataatgctgcg 
               
               
                 cagtgttgccacataaccactatattaaccatttatctagcggacgccaaaaactcaatgta 
               
               
                 tttctgaggaagcgtggtgcataatgccacgcagcgtctgcataacttttattatttctttt 
               
               
                 attaatcaacaaaattttgtttttaacatttcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 
               
               
                 aaaaaaaaaagggtcggcatggcatctccacctcctcgcggtccgacctgggcatccgaagg 
               
               
                 aggacgcacgtccactcggatggctaagggagagccacgagctcctgtttaaaccagctcca 
               
               
                 attcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgac 
               
               
                 tgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctg 
               
               
                 gcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcg 
               
               
                 aatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtg 
               
               
                 accgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgc 
               
               
                 cacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgattta 
               
               
                 gtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggcca 
               
               
                 tcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggact 
               
               
                 cttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataaggga 
               
               
                 ttttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaat 
               
               
                 tttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacc 
               
               
                 cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctg 
               
               
                 ataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccc 
               
               
                 ttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaa 
               
               
                 gtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacag 
               
               
                 cggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaag 
               
               
                 ttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgc 
               
               
                 atacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacgga 
               
               
                 tggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggcca 
               
               
                 acttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggg 
               
               
                 gatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacga 
               
               
                 gcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaac 
               
               
                 tacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcagga 
               
               
                 ccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtga 
               
               
                 gcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtag 
               
               
                 ttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagata 
               
               
                 ggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagat 
               
               
                 tgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctca 
               
               
                 tgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatc 
               
               
                 aaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaacc 
               
               
                 accgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaa 
               
               
                 ctggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccac 
               
               
                 cacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggc 
               
               
                 tgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggata 
               
               
                 aggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacc 
               
               
                 tacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggag 
               
               
                 aaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttc 
               
               
                 cagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgt 
               
               
                 cgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctt 
               
               
                 tttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctg 
               
               
                 attctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacg 
               
               
                 accgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctct 
               
               
                 ccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg 
               
               
                 gcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacac 
               
               
                 tttatgctcccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaa 
               
               
                 cagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctgg 
               
               
                 gtaccggcgcca 
               
               
                   
               
               
                 pVCR modified vector gH FL-SGP gL-SGP gO (SEQ ID NO: 55): 
               
               
                 cgcgtcggctacaattaatacataaccttatgtatcatacacatacgatttaggtgacacta 
               
               
                 tagatgggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcac 
               
               
                 gttgacatcgaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttga 
               
               
                 ggtagaagccaagcaggtcactgataatgaccatgctaatgccagagcgttttcgcatctgg 
               
               
                 cttcaaaactgatcgaaacggaggtggacccatccgacacgatccttgacattggaagtgcg 
               
               
                 cccgcccgcagaatgtattctaagcacaagtatcattgtatctgtccgatgagatgtgcgga 
               
               
                 agatccggacagattgtataagtatgcaactaagctgaagaaaaactgtaaggaaataactg 
               
               
                 ataaggaattggacaagaaaatgaaggagctcgccgccgtcatgagcgaccctgacctggaa 
               
               
                 actgagactatgtgcctccacgacgacgagtcgtgtcgctacgaagggcaagtcgctgttta 
               
               
                 ccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaataagggagtta 
               
               
                 gagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctggagca 
               
               
                 tatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggcct 
               
               
                 atgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatt 
               
               
                 tgaaaccatccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggac 
               
               
                 ttactgaggagctggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatg 
               
               
                 tcggtgtgagactatagttagttgcgacgggtacgtcgttaaaagaatagctatcagtccag 
               
               
                 gcctgtatgggaagccttcaggctatgctgctacgatgcaccgcgagggattcttgtgctgc 
               
               
                 aaagtgacagacacattgaacggggagagggtctcttttcccgtgtgcacgtatgtgccagc 
               
               
                 tacattgtgtgaccaaatgactggcatactggcaacagatgtcagtgcggacgacgcgcaaa 
               
               
                 aactgctggttgggctcaaccagcgtatagtcgtcaacggtcgcacccagagaaacaccaat 
               
               
                 accatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggtgggcaaaggaata 
               
               
                 taaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtcatggggt 
               
               
                 gttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaacc 
               
               
                 atcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacatt 
               
               
                 ggagatcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctc 
               
               
                 tcattaccgccgaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgt 
               
               
                 gaagccgaggagttgcgcgcagctctaccacctttggcagctgatgttgaggagcccactct 
               
               
                 ggaagccgatgtagacttgatgttacaagaggctggggccggctcagtggagacacctcgtg 
               
               
                 gcttgataaaggttaccagctacgctggcgaggacaagatcggctcttacgctgtgctttct 
               
               
                 ccgcaggctgtactcaagagtgaaaaattatcttgcatccaccctctcgctgaacaagtcat 
               
               
                 agtgataacacactctggccgaaaagggcgttatgccgtggaaccataccatggtaaagtag 
               
               
                 tggtgccagagggacatgcaatacccgtccaggactttcaagctctgagtgaaagtgccacc 
               
               
                 attgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccacacatggagg 
               
               
                 agcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcgaat 
               
               
                 acctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctc 
               
               
                 acaggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgacc 
               
               
                 agccgctccttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctg 
               
               
                 gcatcattaaaagcgcagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgt 
               
               
                 gcagaaattataagggacgtcaagaaaatgaaagggctggacgtcaatgccagaactgtgga 
               
               
                 ctcagtgctcttgaatggatgcaaacaccccgtagagaccctgtatattgacgaagcttttg 
               
               
                 cttgtcatgcaggtactctcagagcgctcatagccattataagacctaaaaaggcagtgctc 
               
               
                 tgcggggatcccaaacagtgcggtttttttaacatgatgtgcctgaaagtgcattttaacca 
               
               
                 cgagatttgcacacaagtcttccacaaaagcatctctcgccgttgcactaaatctgtgactt 
               
               
                 cggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccgaaagagactaag 
               
               
                 attgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcacttgttt 
               
               
                 cagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctg 
               
               
                 cctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcct 
               
               
                 ctgtacgcacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgt 
               
               
                 gtggaaaacactagccggcgacccatggataaaaacactgactgccaagtaccctgggaatt 
               
               
                 tcactgccacgatagaggagtggcaagcagagcatgatgccatcatgaggcacatcttggag 
               
               
                 agaccggaccctaccgacgtcttccagaataaggcaaacgtgtgttgggccaaggctttagt 
               
               
                 gccggtgctgaagaccgctggcatagacatgaccactgaacaatggaacactgtggattatt 
               
               
                 ttgaaacggacaaagctcactcagcagagatagtattgaaccaactatgcgtgaggttcttt 
               
               
                 ggactcgatctggactccggtctattttctgcacccactgttccgttatccattaggaataa 
               
               
                 tcactgggataactccccgtcgcctaacatgtacgggctgaataaagaagtggtccgtcagc 
               
               
                 tctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatgacatgaac 
               
               
                 actggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgcc 
               
               
                 tcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagca 
               
               
                 aattgaagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggtt 
               
               
                 gactggttgtcagaccggcctgaggctaccttcagagctcggctggatttaggcatcccagg 
               
               
                 tgatgtgcccaaatatgacataatatttgttaatgtgaggaccccatataaataccatcact 
               
               
                 atcagcagtgtgaagaccatgccattaagcttagcatgttgaccaagaaagcttgtctgcat 
               
               
                 ctgaatcccggcggaacctgtgtcagcataggttatggttacgctgacagggccagcgaaag 
               
               
                 catcattggtgctatagcgcggcagttcaagttttcccgggtatgcaaaccgaaatcctcac 
               
               
                 ttgaagagacggaagttctgtttgtattcattgggtacgatcgcaaggcccgtacgcacaat 
               
               
                 ccttacaagctttcatcaaccttgaccaacatttatacaggttccagactccacgaagccgg 
               
               
                 atgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaaggagtgatta 
               
               
                 taaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataagaaa 
               
               
                 ttcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgc 
               
               
                 agctaaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtg 
               
               
                 acaaacagttggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaag 
               
               
                 tcagtagcgattccactgttgtccaccggcatcttttccgggaacaaagatcgactaaccca 
               
               
                 atcattgaaccatttgctgacagctttagacaccactgatgcagatgtagccatatactgca 
               
               
                 gggacaagaaatgggaaatgactctcaaggaagcagtggctaggagagaagcagtggaggag 
               
               
                 atatgcatatccgacgactcttcagtgacagaacctgatgcagagctggtgagggtgcatcc 
               
               
                 gaagagttctttggctggaaggaagggctacagcacaagcgatggcaaaactttctcatatt 
               
               
                 tggaagggaccaagtttcaccaggcggccaaggatatagcagaaattaatgccatgtggccc 
               
               
                 gttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagcatgagcagtat 
               
               
                 taggtcgaaatgccccgtcgaagagtcggaagcctcctcaccacctagcacgctgccttgct 
               
               
                 tgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaa 
               
               
                 attactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatcca 
               
               
                 atgctcccagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatc 
               
               
                 tcgtggaaacaccaccggtagacgagactccggagccatcggcagagaaccaatccacagag 
               
               
                 gggacacctgaacaaccaccacttataaccgaggatgagaccaggactagaacgcctgagcc 
               
               
                 gatcatcatcgaagaggaagaagaggatagcataagtttgctgtcagatggcccgacccacc 
               
               
                 aggtgctgcaagtcgaggcagacattcacgggccgccctctgtatctagctcatcctggtcc 
               
               
                 attcctcatgcatccgactttgatgtggacagtttatccatacttgacaccctggagggagc 
               
               
                 tagcgtgaccagcggggcaacgtcagccgagactaactcttacttcgcaaagagtatggagt 
               
               
                 ttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctccacatcccgctccg 
               
               
                 cgcacaagaacaccgtcacttgcacccagcagggcctgctcgagagggatcacgggagaaac 
               
               
                 cgtgggatacgcggttacacacaatagcgagggcttcttgctatgcaaagttactgacacag 
               
               
                 taaaaggagaacgggtatcgttccctgtgtgcacgtacatcccggccaccataaactcgaga 
               
               
                 accagcctggtctccaacccgccaggcgtaaatagggtgattacaagagaggagtttgaggc 
               
               
                 gttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatcttttcctccgacaccg 
               
               
                 gtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtgttggag 
               
               
                 aggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtgg 
               
               
                 agaacatgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggca 
               
               
                 gaaggaaaagtggagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaa 
               
               
                 ccgtgccttttcaagccccaaggtcgcagtggaagcctgtaacgccatgttgaaagagaact 
               
               
                 ttccgactgtggcttcttactgtattattccagagtacgatgcctatttggacatggttgac 
               
               
                 ggagcttcatgctgcttagacactgccagtttttgccctgcaaagctgcgcagctttccaaa 
               
               
                 gaaacactcctatttggaacccacaatacgatcggcagtgccttcagcgatccagaacacgc 
               
               
                 tccagaacgtcctggcagctgccacaaaaagaaattgcaatgtcacgcaaatgagagaattg 
               
               
                 cccgtattggattcggcggcctttaatgtggaatgcttcaagaaatatgcgtgtaataatga 
               
               
                 atattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggtaaattaca 
               
               
                 ttaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaatatg 
               
               
                 ttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactcc 
               
               
                 aggaacaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctag 
               
               
                 caacagcgtatctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgctt 
               
               
                 ccgaacattcatacactgtttgatatgtcggctgaagactttgacgctattatagccgagca 
               
               
                 cttccagcctggggattgtgttctggaaactgacatcgcgtcgtttgataaaagtgaggacg 
               
               
                 acgccatggctctgaccgcgttaatgattctggaagacttaggtgtggacgcagagctgttg 
               
               
                 acgctgattgaggcggctttcggcgaaatttcatcaatacatttgcccactaaaactaaatt 
               
               
                 taaattcggagccatgatgaaatctggaatgttcctcacactgtttgtgaacacagtcatta 
               
               
                 acattgtaatcgcaagcagagtgttgagagaacggctaaccggatcaccatgtgcagcattc 
               
               
                 attggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcagacaggtgcgc 
               
               
                 cacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgccttatt 
               
               
                 tctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagacccc 
               
               
                 ctaaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacag 
               
               
                 gagaagggcattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgt 
               
               
                 gcaaggcagtagaatcaaggtatgaaaccgtaggaacttccatcatagttatggccatgact 
               
               
                 actctagctagcagtgttaaatcattcagctacctgagaggggcccctataactctctacgg 
               
               
                 ctaacctgaatggactacgacatagtctagtcgacgccaccatgaggcctggcctgccctcc 
               
               
                 tacctgatcatcctggccgtgtgcctgttcagccacctgctgtccagcagatacggcgccga 
               
               
                 ggccgtgagcgagcccctggacaaggctttccacctgctgctgaacacctacggcagaccca 
               
               
                 tccggtttctgcgggagaacaccacccagtgcacctacaacagcagcctgcggaacagcacc 
               
               
                 gtcgtgagagagaacgccatcagcttcaactttttccagagctacaaccagtactacgtgtt 
               
               
                 ccacatgcccagatgcctgtttgccggccctctggccgagcagttcctgaaccaggtggacc 
               
               
                 tgaccgagacactggaaagataccagcagcggctgaatacctacgccctggtgtccaaggac 
               
               
                 ctggccagctaccggtcctttagccagcagctcaaggctcaggatagcctcggcgagcagcc 
               
               
                 taccaccgtgccccctcccatcgacctgagcatcccccacgtgtggatgcctccccagacca 
               
               
                 cccctcacggctggaccgagagccacaccacctccggcctgcacagaccccacttcaaccag 
               
               
                 acctgcatcctgttcgacggccacgacctgctgtttagcaccgtgaccccctgcctgcacca 
               
               
                 gggcttctacctgatcgacgagctgagatacgtgaagatcaccctgaccgaggatttcttcg 
               
               
                 tggtcaccgtgtccatcgacgacgacacccccatgctgctgatcttcggccacctgcccaga 
               
               
                 gtgctgttcaaggccccctaccagcgggacaacttcatcctgcggcagaccgagaagcacga 
               
               
                 gctgctggtgctggtcaagaaggaccagctgaaccggcactcctacctgaaggaccccgact 
               
               
                 tcctggacgccgccctggacttcaactacctggacctgagcgccctgctgagaaacagcttc 
               
               
                 cacagatacgccgtggacgtgctgaagtccggacggtgccagatgctcgatcggcggaccgt 
               
               
                 ggagatggccttcgcctatgccctcgccctgttcgccgctgccagacaggaagaggctggcg 
               
               
                 cccaggtgtcagtgcccagagccctggatagacaggccgccctgctgcagatccaggaattc 
               
               
                 atgatcacctgcctgagccagaccccccctagaaccaccctgctgctgtaccccacagccgt 
               
               
                 ggatctggccaagagggccctgtggacccccaaccagatcaccgacatcacaagcctcgtgc 
               
               
                 ggctcgtgtacatcctgagcaagcagaaccagcagcacctgatcccccagtgggccctgaga 
               
               
                 cagatcgccgacttcgccctgaagctgcacaagacccatctggccagctttctgagcgcctt 
               
               
                 cgccaggcaggaactgtacctgatgggcagcctggtccacagcatgctggtgcataccaccg 
               
               
                 agcggcgggagatcttcatcgtggagacaggcctgtgtagcctggccgagctgtcccacttt 
               
               
                 acccagctgctggcccaccctcaccacgagtacctgagcgacctgtacaccccctgcagcag 
               
               
                 cagcggcagacgggaccacagcctggaacggctgaccagactgttccccgatgccaccgtgc 
               
               
                 ctgctacagtgcctgccgccctgtccatcctgtccaccatgcagcccagcaccctggaaacc 
               
               
                 ttccccgacctgttctgcctgcccctgggcgagagctttagcgccctgaccgtgtccgagca 
               
               
                 cgtgtcctacatcgtgaccaatcagtacctgatcaagggcatcagctaccccgtgtccacca 
               
               
                 cagtcgtgggccagagcctgatcatcacccagaccgacagccagaccaagtgcgagctgacc 
               
               
                 cggaacatgcacaccacacacagcatcaccgtggccctgaacatcagcctggaaaactgcgc 
               
               
                 tttctgtcagtctgccctgctggaatacgacgatacccagggcgtgatcaacatcatgtaca 
               
               
                 tgcacgacagcgacgacgtgctgttcgccctggacccctacaacgaggtggtggtgtccagc 
               
               
                 ccccggacccactacctgatgctgctgaagaacggcaccgtgctggaagtgaccgacgtggt 
               
               
                 ggtggacgccaccgacagcagactgctgatgatgagcgtgtacgccctgagcgccatcatcg 
               
               
                 gcatctacctgctgtaccggatgctgaaaacctgctgataatctagacggcgcgcccaccca 
               
               
                 gcggccgcctataactctctacggctaacctgaatggactacgacatagtctagtcgacgcc 
               
               
                 accatgtgcagaaggcccgactgcggcttcagcttcagccctggacccgtgatcctgctgtg 
               
               
                 gtgctgcctgctgctgcctatcgtgtcctctgccgccgtgtctgtggcccctacagccgccg 
               
               
                 agaaggtgccagccgagtgccccgagctgaccagaagatgcctgctgggcgaggtgttcgag 
               
               
                 ggcgacaagtacgagagctggctgcggcccctggtcaacgtgaccggcagagatggccccct 
               
               
                 gagccagctgatccggtacagacccgtgacccccgaggccgccaatagcgtgctgctggacg 
               
               
                 aggccttcctggataccctggccctgctgtacaacaaccccgaccagctgagagccctgctg 
               
               
                 accctgctgtccagcgacaccgcccccagatggatgaccgtgatgcggggctacagcgagtg 
               
               
                 tggagatggcagccctgccgtgtacacctgcgtggacgacctgtgcagaggctacgacctga 
               
               
                 ccagactgagctacggccggtccatcttcacagagcacgtgctgggcttcgagctggtgccc 
               
               
                 cccagcctgttcaacgtggtggtggccatccggaacgaggccaccagaaccaacagagccgt 
               
               
                 gcggctgcctgtgtctacagccgctgcacctgagggcatcacactgttctacggcctgtaca 
               
               
                 acgccgtgaaagagttctgcctccggcaccagctggatccccccctgctgagacacctggac 
               
               
                 aagtactacgccggcctgcccccagagctgaagcagaccagagtgaacctgcccgcccacag 
               
               
                 cagatatggccctcaggccgtggacgccagatgataatctagacggcgcgcccacccaatcg 
               
               
                 atctataactctctacggctaacctgaatggactacgacatagtctagtcgacgccaccatg 
               
               
                 ggcaagaaagaaatgatcatggtcaagggcatccccaagatcatgctgctgattagcatcac 
               
               
                 ctttctgctgctgtccctgatcaactgcaacgtgctggtcaacagccggggcaccagaagat 
               
               
                 cctggccctacaccgtgctgtcctaccggggcaaagagatcctgaagaagcagaaagaggac 
               
               
                 atcctgaagcggctgatgagcaccagcagcgacggctaccggttcctgatgtaccccagcca 
               
               
                 gcagaaattccacgccatcgtgatcagcatggacaagttcccccaggactacatcctggccg 
               
               
                 gacccatccggaacgacagcatcacccacatgtggttcgacttctacagcacccagctgcgg 
               
               
                 aagcccgccaaatacgtgtacagcgagtacaaccacaccgcccacaagatcaccctgaggcc 
               
               
                 tcccccttgtggcaccgtgcccagcatgaactgcctgagcgagatgctgaacgtgtccaagc 
               
               
                 ggaacgacaccggcgagaagggctgcggcaacttcaccaccttcaaccccatgttcttcaac 
               
               
                 gtgccccggtggaacaccaagctgtacatcggcagcaacaaagtgaacgtggacagccagac 
               
               
                 catctactttctgggcctgaccgccctgctgctgagatacgcccagcggaactgcacccggt 
               
               
                 ccttctacctggtcaacgccatgagccggaacctgttccgggtgcccaagtacatcaacggc 
               
               
                 accaagctgaagaacaccatgcggaagctgaagcggaagcaggccctggtcaaagagcagcc 
               
               
                 ccagaagaagaacaagaagtcccagagcaccaccaccccctacctgagctacaccacctcca 
               
               
                 ccgccttcaacgtgaccaccaacgtgacctacagcgccacagccgccgtgaccagagtggcc 
               
               
                 acaagcaccaccggctaccggcccgacagcaactttatgaagtccatcatggccacccagct 
               
               
                 gagagatctggccacctgggtgtacaccaccctgcggtacagaaacgagcccttctgcaagc 
               
               
                 ccgaccggaacagaaccgccgtgagcgagttcatgaagaatacccacgtgctgatcagaaac 
               
               
                 gagacaccctacaccatctacggcaccctggacatgagcagcctgtactacaacgagacaat 
               
               
                 gagcgtggagaacgagacagccagcgacaacaacgaaaccacccccacctcccccagcaccc 
               
               
                 ggttccagcggaccttcatcgaccccctgtgggactacctggacagcctgctgttcctggac 
               
               
                 aagatccggaacttcagcctgcagctgcccgcctacggcaatctgaccccccctgagcacag 
               
               
                 aagggccgccaacctgagcaccctgaacagcctgtggtggtggagccagtgataatctagac 
               
               
                 ggcgcgcccacccaccgcgggcaatatagcaacactaaaaactcgatgtacttccgaggaag 
               
               
                 cgcagtgcataatgctgcgcagtgttgccacataaccactatattaaccatttatctagcgg 
               
               
                 acgccaaaaactcaatgtatttctgaggaagcgtggtgcataatgccacgcagcgtctgcat 
               
               
                 aacttttattatttcttttattaatcaacaaaattttgtttttaacatttcaaaaaaaaaaa 
               
               
                 aaaaaaaaaaaaaaaaaaaaaaaaaaaaagggtcggcatggcatctccacctcctcgcggtc 
               
               
                 cgacctgggcatccgaaggaggacgcacgtccactcggatggctaagggagagccacgagct 
               
               
                 cctgtttaaaccagctccaattcgccctatagtgagtcgtattacgcgcgctcactggccgt 
               
               
                 cgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcac 
               
               
                 atccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacag 
               
               
                 ttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgt 
               
               
                 ggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctt 
               
               
                 tcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctc 
               
               
                 cctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtga 
               
               
                 tggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtcca 
               
               
                 cgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctat 
               
               
                 tcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta 
               
               
                 acaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcg 
               
               
                 gggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgc 
               
               
                 tcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtatt 
               
               
                 caacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctca 
               
               
                 cccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttaca 
               
               
                 tcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttcca 
               
               
                 atgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggca 
               
               
                 agagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtca 
               
               
                 cagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatg 
               
               
                 agtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgc 
               
               
                 ttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatg 
               
               
                 aagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgc 
               
               
                 aaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatgga 
               
               
                 ggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctg 
               
               
                 ataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggt 
               
               
                 aagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaa 
               
               
                 tagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagttt 
               
               
                 actcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaag 
               
               
                 atcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtc 
               
               
                 agaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgct 
               
               
                 gcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctacca 
               
               
                 actctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagt 
               
               
                 gtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgc 
               
               
                 taatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactca 
               
               
                 agacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcc 
               
               
                 cagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcg 
               
               
                 ccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacagga 
               
               
                 gagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcg 
               
               
                 ccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaa 
               
               
                 acgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttc 
               
               
                 tttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgatac 
               
               
                 cgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcc 
               
               
                 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg 
               
               
                 tttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatta 
               
               
                 ggcaccccaggctttacactttatgctcccggctcgtatgttgtgtggaattgtgagcggat 
               
               
                 aacaatttcacacaggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcac 
               
               
                 taaagggaacaaaagctgggtaccggcgcca 
               
               
                   
               
               
                 A526 Vector: SGP-gH-SGP-gL-SGP-UL128-2A-UL130-2Amod-UL131 (SEQ ID NO: 56): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTAGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGGCCTGGCCT   
               
               
                 
                   GCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGT 
                 
               
               
                 
                   GAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAA 
                 
               
               
                 
                   CACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTT 
                 
               
               
                 
                   TTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTT 
                 
               
               
                 
                   CCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAA 
                 
               
               
                 
                   GGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGT 
                 
               
               
                 
                   GCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAG 
                 
               
               
                 
                   CCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTT 
                 
               
               
                 
                   TAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGAC 
                 
               
               
                 
                   CGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAG 
                 
               
               
                 
                   AGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCT 
                 
               
               
                 
                   GGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAA 
                 
               
               
                 
                   CTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTG 
                 
               
               
                 
                   CCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGA 
                 
               
               
                 
                   AGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCAT 
                 
               
               
                 
                   GATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAG 
                 
               
               
                 
                   GGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAA 
                 
               
               
                 
                   CCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCT 
                 
               
               
                 
                   GGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCA 
                 
               
               
                 
                   TACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCA 
                 
               
               
                 
                   GCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCA 
                 
               
               
                 
                   CAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCT 
                 
               
               
                 
                   GTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGC 
                 
               
               
                 
                   CCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTC 
                 
               
               
                 
                   CACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACAT 
                 
               
               
                 
                   GCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCT 
                 
               
               
                 
                   GGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGA 
                 
               
               
                 
                   CCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGA 
                 
               
               
                 
                   AGTGACCGACGTGGTGGTGGACGCCACCGACAGCAGACTGCTGATGATGAGCGTGTACGCCCTGAGCGCCATCAT 
                 
               
               
                   CGGCATCTACCTGCTGTACCGGATGCTGAAAACCTGC TGATAATCTAGAGGCCCCTATAACTCTCTACGGCTAAC 
               
               
                 CTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGTGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGCCCTGG   
               
               
                 
                   ACCCGTGATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCTACAGC 
                 
               
               
                 
                   CGCCGAGAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGCGACAA 
                 
               
               
                 
                   GTACGAGAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGATGGCCCCCTGAGCCAGCTGATCCGGTACAG 
                 
               
               
                 
                   ACCCGTGACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGCCTTCCTGGATACCCTGGCCCTGCTGTACAA 
                 
               
               
                 
                   CAACCCCGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGACACCGCCCCCAGATGGATGACCGTGATGCG 
                 
               
               
                 GGGCTACAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTGCGTGGACGACCTGTGCAGAGGCTACGACCT 
               
               
                 
                   GACCAGACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGCCTGTT 
                 
               
               
                 
                   CAACGTGGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAGAGCCGTGCGGCTGCCTGTGTCTACAGCCGC 
                 
               
               
                 
                   TGCACCTGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGTGAAAGAGTTCTGCCTCCGGCACCAGCTGGA 
                 
               
               
                 
                   TCCCCCCCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTGAACCT 
                 
               
               
                   GCCCGCCCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGA TGATAACGCCGGCGGCCCCTATAACTCTCTAC 
               
               
                 GGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGCCCCAAGGACCTGACCCCCTTCCTGACAA   
               
               
                 
                   CCCTGTGGCTGCTCCTGGGCCATAGCAGAGTGCCTAGAGTGCGGGCCGAGGAATGCTGCGAGTTCATCAACGTGA 
                 
               
               
                 
                   ACCACCCCCCCGAGCGGTGCTACGACTTCAAGATGTGCAACCGGTTCACCGTGGCCCTGAGATGCCCCGACGGCG 
                 
               
               
                 
                   AAGTGTGCTACAGCCCCGAGAAAACCGCCGAGATCCGGGGCATCGTGACCACCATGACCCACAGCCTGACCCGGC 
                 
               
               
                 
                   AGGTGGTGCACAACAAGCTGACCAGCTGCAACTACAACCCCCTGTACCTGGAAGCCGACGGCCGGATCAGATGCG 
                 
               
               
                 
                   GCAAAGTGAACGACAAGGCCCAGTACCTGCTGGGAGCCGCCGGAAGCGTGCCCTACCGGTGGATCAACCTGGAAT 
                 
               
               
                 
                   ACGACAAGATCACCCGGATCGTGGGCCTGGACCAGTACCTGGAAAGCGTGAAGAAGCACAAGCGGCTGGACGTGT 
                 
               
               
                   GCAGAGCCAAGATGGGCTACATGCTGCAG CTGTTGAATTTTGACCTTCTTAAGCTTGCGGGAGACGTCGAGTCCA 
               
               
                 ACCCCGGGCCC ATGCTGCGGCTGCTGCTGAGACACCACTTCCACTGCCTGCTGCTGTGTGCCGTGTGGGCCACCC   
               
               
                 
                   CTTGTCTGGCCAGCCCTTGGAGCACCCTGACCGCCAACCAGAACCCTAGCCCCCCTTGGTCCAAGCTGACCTACA 
                 
               
               
                 
                   GCAAGCCCCACGACGCCGCCACCTTCTACTGCCCCTTTCTGTACCCCAGCCCTCCCAGAAGCCCCCTGCAGTTCA 
                 
               
               
                 
                   GCGGCTTCCAGAGAGTGTCCACCGGCCCTGAGTGCCGGAACGAGACACTGTACCTGCTGTACAACCGGGAGGGCC 
                 
               
               
                 
                   AGACACTGGTGGAGCGGAGCAGCACCTGGGTGAAAAAAGTGATCTGGTATCTGAGCGGCCGGAACCAGACCATCC 
                 
               
               
                 
                   TGCAGCGGATGCCCAGAACCGCCAGCAAGCCCAGCGACGGCAACGTGCAGATCAGCGTGGAGGACGCCAAAATCT 
                 
               
               
                 
                   TCGGCGCCCACATGGTGCCCAAGCAGACCAAGCTGCTGAGATTCGTGGTCAACGACGGCACCAGATATCAGATGT 
                 
               
               
                 
                   GCGTGATGAAGCTGGAAAGCTGGGCCCACGTGTTCCGGGACTACTCCGTGAGCTTCCAGGTCCGGCTGACCTTCA 
                 
               
               
                   CCGAGGCCAACAACCAGACCTACACCTTCTGCACCCACCCCAACCTGATCGTG CTGCTGAACTTCGACCTGCTGA 
               
               
                 AGCTGGCCGGCGACGTGGAGAGCAACCCCGGCCCCCAT ATGCGGCTGTGCAGAGTGTGGCTGTCCGTGTGCCTGT   
               
               
                 
                   GTGCCGTGGTGCTGGGCCAGTGCCAGAGAGAGACAGCCGAGAAGAACGACTACTACCGGGTGCCCCACTACTGGG 
                 
               
               
                 
                   ATGCCTGCAGCAGAGCCCTGCCCGACCAGACCCGGTACAAATACGTGGAGCAGCTCGTGGACCTGACCCTGAACT 
                 
               
               
                 
                   ACCACTACGACGCCAGCCACGGCCTGGACAACTTCGACGTGCTGAAGCGGATCAACGTGACCGAGGTGTCCCTGC 
                 
               
               
                 
                   TGATCAGCGACTTCCGGCGGCAGAACAGAAGAGGCGGCACCAACAAGCGGACCACCTTCAACGCCGCTGGCTCTC 
                 
               
               
                   TGGCCCCTCACGCCAGATCCCTGGAATTCAGCGTGCGGCTGTTCGCCAAC TGATAACGTTGCATCCTGCAGGATA 
               
               
                 CAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTT 
               
               
                 TCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 
               
               
                 GGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGC 
               
               
                 TAAGGGAGAGCCACGTTTAAACGCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTAC 
               
               
                 TGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTT 
               
               
                 GAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACA 
               
               
                 ACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTCTCATCAACC 
               
               
                 GTGGCTCCCTCACTTTCTGGCTGGATGATGGGGCGATTCAGGCCTGGTATGAGTCAGCAACACCTTCTTCACGAG 
               
               
                 GCAGACCTCAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCTTCA 
               
               
                 TGTGGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTC 
               
               
                 ACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGAGATTTCCTGGAA 
               
               
                 GATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGGCCGCGGCAAAGCCGTTTTTCCATAGGCTCCGCCCCCCTG 
               
               
                 ACAAGCATCACGAAATCTGACGCTCAAATCAGTGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC 
               
               
                 CCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCATTCCGCTGTTATGGCCGCG 
               
               
                 TTTGTCTCATTCCACGCCTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTATGCACGAACCCC 
               
               
                 CCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGAAAGACATGCAAAAGCAC 
               
               
                 CACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGAAGTCATGCGCCGGTTAAGGCTAAACTGAA 
               
               
                 AGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGCTCAGAGAACCTTC 
               
               
                 GAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAACGATCTCAAGAA 
               
               
                 GATCATCTTATTAAGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCA 
               
               
                 AAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACT 
               
               
                 TGGTCTGACAGTTATTAGAAAAATTCATCCAGCAGACGATAAAACGCAATACGCTGGCTATCCGGTGCCGCAATG 
               
               
                 CCATACAGCACCAGAAAACGATCCGCCCATTCGCCGCCCAGTTCTTCCGCAATATCACGGGTGGCCAGCGCAATA 
               
               
                 TCCTGATAACGATCCGCCACGCCCAGACGGCCGCAATCAATAAAGCCGCTAAAACGGCCATTTTCCACCATAATG 
               
               
                 TTCGGCAGGCACGCATCACCATGGGTCACCACCAGATCTTCGCCATCCGGCATGCTCGCTTTCAGACGCGCAAAC 
               
               
                 AGCTCTGCCGGTGCCAGGCCCTGATGTTCTTCATCCAGATCATCCTGATCCACCAGGCCCGCTTCCATACGGGTA 
               
               
                 CGCGCACGTTCAATACGATGTTTCGCCTGATGATCAAACGGACAGGTCGCCGGGTCCAGGGTATGCAGACGACGC 
               
               
                 ATGGCATCCGCCATAATGCTCACTTTTTCTGCCGGCGCCAGATGGCTAGACAGCAGATCCTGACCCGGCACTTCG 
               
               
                 CCCAGCAGCAGCCAATCACGGCCCGCTTCGGTCACCACATCCAGCACCGCCGCACACGGAACACCGGTGGTGGCC 
               
               
                 AGCCAGCTCAGACGCGCCGCTTCATCCTGCAGCTCGTTCAGCGCACCGCTCAGATCGGTTTTCACAAACAGCACC 
               
               
                 GGACGACCCTGCGCGCTCAGACGAAACACCGCCGCATCAGAGCAGCCAATGGTCTGCTGCGCCCAATCATAGCCA 
               
               
                 AACAGACGTTCCACCCACGCTGCCGGGCTACCCGCATGCAGGCCATCCTGTTCAATCATACTCTTCCTTTTTCAA 
               
               
                 TATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA 
               
               
                 ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGT 
               
               
                 TAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAAT 
               
               
                 AGACCGAGATAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGG 
               
               
                 CGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGT 
               
               
                 AACGCCAGGGTTTTCCCAGTCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A527 Vector: SGP-gH-SGP-gL-SGP-UL128-EMCV-UL130-EV71-UL131 (SEQ ID 
               
               
                 NO: 57): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTAGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGGCCTGGCCT   
               
               
                 
                   GCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGT 
                 
               
               
                 
                   GAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAA 
                 
               
               
                 
                   CACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTT 
                 
               
               
                 
                   TTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTT 
                 
               
               
                 
                   CCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAA 
                 
               
               
                 
                   GGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGT 
                 
               
               
                 
                   GCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAG 
                 
               
               
                 
                   CCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTT 
                 
               
               
                 
                   TAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGAC 
                 
               
               
                 
                   CGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAG 
                 
               
               
                 
                   AGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCT 
                 
               
               
                 
                   GGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAA 
                 
               
               
                 
                   CTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTG 
                 
               
               
                 
                   CCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGA 
                 
               
               
                 
                   AGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCAT 
                 
               
               
                 
                   GATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAG 
                 
               
               
                 
                   GGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAA 
                 
               
               
                 
                   CCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCT 
                 
               
               
                 
                   GGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCA 
                 
               
               
                 
                   TACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCA 
                 
               
               
                 
                   GCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCA 
                 
               
               
                 
                   CAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCT 
                 
               
               
                 
                   GTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGC 
                 
               
               
                 
                   CCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTC 
                 
               
               
                 
                   CACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACAT 
                 
               
               
                 
                   GCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCT 
                 
               
               
                 GGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGA 
               
               
                 
                   CCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGA 
                 
               
               
                 
                   AGTGACCGACGTGGTGGTGGACGCCACCGACAGCAGACTGCTGATGATGAGCGTGTACGCCCTGAGCGCCATCAT 
                 
               
               
                   CGGCATCTACCTGCTGTACCGGATGCTGAAAACCTGC TGATAATCTAGAGGCCCCTATAACTCTCTACGGCTAAC 
               
               
                 CTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGTGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGCCCTGG   
               
               
                 
                   ACCCGTGATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCTACAGC 
                 
               
               
                 
                   CGCCGAGAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGCGACAA 
                 
               
               
                 
                   GTACGAGAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGATGGCCCCCTGAGCCAGCTGATCCGGTACAG 
                 
               
               
                 
                   ACCCGTGACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGCCTTCCTGGATACCCTGGCCCTGCTGTACAA 
                 
               
               
                 
                   CAACCCCGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGACACCGCCCCCAGATGGATGACCGTGATGCG 
                 
               
               
                 
                   GGGCTACAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTGCGTGGACGACCTGTGCAGAGGCTACGACCT 
                 
               
               
                 
                   GACCAGACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGCCTGTT 
                 
               
               
                 
                   CAACGTGGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAGAGCCGTGCGGCTGCCTGTGTCTACAGCCGC 
                 
               
               
                 
                   TGCACCTGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGTGAAAGAGTTCTGCCTCCGGCACCAGCTGGA 
                 
               
               
                 
                   TCCCCCCCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTGAACCT 
                 
               
               
                   GCCCGCCCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGA TGATAACGCCGGCGGCCCCTATAACTCTCTAC 
               
               
                 GGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGCCCCAAGGACCTGACCCCCTTCCTGACAA   
               
               
                 
                   CCCTGTGGCTGCTCCTGGGCCATAGCAGAGTGCCTAGAGTGCGGGCCGAGGAATGCTGCGAGTTCATCAACGTGA 
                 
               
               
                 
                   ACCACCCCCCCGAGCGGTGCTACGACTTCAAGATGTGCAACCGGTTCACCGTGGCCCTGAGATGCCCCGACGGCG 
                 
               
               
                 
                   AAGTGTGCTACAGCCCCGAGAAAACCGCCGAGATCCGGGGCATCGTGACCACCATGACCCACAGCCTGACCCGGC 
                 
               
               
                 
                   AGGTGGTGCACAACAAGCTGACCAGCTGCAACTACAACCCCCTGTACCTGGAAGCCGACGGCCGGATCAGATGCG 
                 
               
               
                 
                   GCAAAGTGAACGACAAGGCCCAGTACCTGCTGGGAGCCGCCGGAAGCGTGCCCTACCGGTGGATCAACCTGGAAT 
                 
               
               
                 
                   ACGACAAGATCACCCGGATCGTGGGCCTGGACCAGTACCTGGAAAGCGTGAAGAAGCACAAGCGGCTGGACGTGT 
                 
               
               
                   GCAGAGCCAAGATGGGCTACATGCTGCAG TGATAAGGCGCGCCAACGTTACTGGCCGAAGCCGCTTGGAATAAGG 
               
               
                 CCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGG 
               
               
                 CCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGT 
               
               
                 GAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCC 
               
               
                 CCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCA 
               
               
                 GTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAA 
               
               
                 GGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTC 
               
               
                 GAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATAAT ATGCTGC   
               
               
                 
                   GGCTGCTGCTGAGACACCACTTCCACTGCCTGCTGCTGTGTGCCGTGTGGGCCACCCCTTGTCTGGCCAGCCCTT 
                 
               
               
                 
                   GGAGCACCCTGACCGCCAACCAGAACCCTAGCCCCCCTTGGTCCAAGCTGACCTACAGCAAGCCCCACGACGCCG 
                 
               
               
                 
                   CCACCTTCTACTGCCCCTTTCTGTACCCCAGCCCTCCCAGAAGCCCCCTGCAGTTCAGCGGCTTCCAGAGAGTGT 
                 
               
               
                 
                   CCACCGGCCCTGAGTGCCGGAACGAGACACTGTACCTGCTGTACAACCGGGAGGGCCAGACACTGGTGGAGCGGA 
                 
               
               
                 
                   GCAGCACCTGGGTGAAAAAAGTGATCTGGTATCTGAGCGGCCGGAACCAGACCATCCTGCAGCGGATGCCCAGAA 
                 
               
               
                 
                   CCGCCAGCAAGCCCAGCGACGGCAACGTGCAGATCAGCGTGGAGGACGCCAAAATCTTCGGAGCCCACATGGTGC 
                 
               
               
                 
                   CCAAGCAGACCAAGCTGCTGAGATTCGTGGTCAACGACGGCACCAGATATCAGATGTGCGTGATGAAGCTGGAAA 
                 
               
               
                 
                   GCTGGGCCCACGTGTTCCGGGACTACTCCGTGAGCTTCCAGGTCCGGCTGACCTTCACCGAGGCCAACAACCAGA 
                 
               
               
                   CCTACACCTTCTGCACCCACCCCAACCTGATCGTG TGATAAGTACCTTTGTACGCCTGTTTTATACCCCCTCCCT 
               
               
                 GATTTGCAACTTAGAAGCAACGCAAACCAGATCAATAGTAGGTGTGACATACCAGTCGCATCTTGATCAAGCACT 
               
               
                 TCTGTATCCCCGGACCGAGTATCAATAGACTGTGCACACGGTTGAAGGAGAAAACGTCCGTTACCCGGCTAACTA 
               
               
                 CTTCGAGAAGCCTAGTAACGCCATTGAAGTTGCAGAGTGTTTCGCTCAGCACTCCCCCCGTGTAGATCAGGTCGA 
               
               
                 TGAGTCACCGCATTCCCCACGGGCGACCGTGGCGGTGGCTGCGTTGGCGGCCTGCCTATGGGGTAACCCATAGGA 
               
               
                 CGCTCTAATACGGACATGGCGTGAAGAGTCTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGCTAATC 
               
               
                 CTAACTGCGGAGCACATACCCTTAATCCAAAGGGCAGTGTGTCGTAACGGGCAACTCTGCAGCGGAACCGACTAC 
               
               
                 TTTGGGTGTCCGTGTTTCTTTTTATTCTTGTATTGGCTGCTTATGGTGACAATTAAAGAATTGTTACCATATAGC 
               
               
                 TATTGGATTGGCCATCCAGTGTCAAACAGAGCTATTGTATATCTCTTTGTTGGATTCACACCTCTCACTCTTGAA 
               
               
                 ACGTTACACACCCTCAATTACATTATACTGCTGAACACGAAGCGCAT ATGCGGCTGTGCAGAGTGTGGCTGTCCG   
               
               
                 
                   TGTGCCTGTGTGCCGTGGTGCTGGGCCAGTGCCAGAGAGAGACAGCCGAGAAGAACGACTACTACCGGGTGCCCC 
                 
               
               
                 
                   ACTACTGGGATGCCTGCAGCAGAGCCCTGCCCGACCAGACCCGGTACAAATACGTGGAGCAGCTCGTGGACCTGA 
                 
               
               
                 
                   CCCTGAACTACCACTACGACGCCAGCCACGGCCTGGACAACTTCGACGTGCTGAAGCGGATCAACGTGACCGAGG 
                 
               
               
                 
                   TGTCCCTGCTGATCAGCGACTTCCGGCGGCAGAACAGAAGAGGCGGCACCAACAAGCGGACCACCTTCAACGCCG 
                 
               
               
                   CTGGCTCTCTGGCCCCTCACGCCAGATCCCTGGAATTCAGCGTGCGGCTGTTCGCCAAC TGATAACGTTGCATCC 
               
               
                 TGCAGGATACAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTA 
               
               
                 TTTTATTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAA 
               
               
                 AAAAAAAAGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCAC 
               
               
                 TCGGATGGCTAAGGGAGAGCCACGTTTAAACGCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCC 
               
               
                 TTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATC 
               
               
                 AGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATC 
               
               
                 TTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTC 
               
               
                 TCATCAACCGTGGCTCCCTCACTTTCTGGCTGGATGATGGGGCGATTCAGGCCTGGTATGAGTCAGCAACACCTT 
               
               
                 CTTCACGAGGCAGACCTCAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACTGATGAGGGTGTCAGTGA 
               
               
                 AGTGCTTCATGTGGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAGCAGAATATGTGATACAGGATATATTCCGCT 
               
               
                 TCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGAGAT 
               
               
                 TTCCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGGCCGCGGCAAAGCCGTTTTTCCATAGGCTCC 
               
               
                 GCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGTGGTGGCGAAACCCGACAGGACTATAAAGATACC 
               
               
                 AGGCGTTTCCCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCATTCCGCTGTT 
               
               
                 ATGGCCGCGTTTGTCTCATTCCACGCCTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTATGC 
               
               
                 ACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGAAAGACATG 
               
               
                 CAAAAGCACCACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGAAGTCATGCGCCGGTTAAGGC 
               
               
                 TAAACTGAAAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGCTCAG 
               
               
                 AGAACCTTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAACGA 
               
               
                 TCTCAAGAAGATCATCTTATTAAGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATG 
               
               
                 AGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATAT 
               
               
                 GAGTAAACTTGGTCTGACAGTTATTAGAAAAATTCATCCAGCAGACGATAAAACGCAATACGCTGGCTATCCGGT 
               
               
                 GCCGCAATGCCATACAGCACCAGAAAACGATCCGCCCATTCGCCGCCCAGTTCTTCCGCAATATCACGGGTGGCC 
               
               
                 AGCGCAATATCCTGATAACGATCCGCCACGCCCAGACGGCCGCAATCAATAAAGCCGCTAAAACGGCCATTTTCC 
               
               
                 ACCATAATGTTCGGCAGGCACGCATCACCATGGGTCACCACCAGATCTTCGCCATCCGGCATGCTCGCTTTCAGA 
               
               
                 CGCGCAAACAGCTCTGCCGGTGCCAGGCCCTGATGTTCTTCATCCAGATCATCCTGATCCACCAGGCCCGCTTCC 
               
               
                 ATACGGGTACGCGCACGTTCAATACGATGTTTCGCCTGATGATCAAACGGACAGGTCGCCGGGTCCAGGGTATGC 
               
               
                 AGACGACGCATGGCATCCGCCATAATGCTCACTTTTTCTGCCGGCGCCAGATGGCTAGACAGCAGATCCTGACCC 
               
               
                 GGCACTTCGCCCAGCAGCAGCCAATCACGGCCCGCTTCGGTCACCACATCCAGCACCGCCGCACACGGAACACCG 
               
               
                 GTGGTGGCCAGCCAGCTCAGACGCGCCGCTTCATCCTGCAGCTCGTTCAGCGCACCGCTCAGATCGGTTTTCACA 
               
               
                 AACAGCACCGGACGACCCTGCGCGCTCAGACGAAACACCGCCGCATCAGAGCAGCCAATGGTCTGCTGCGCCCAA 
               
               
                 TCATAGCCAAACAGACGTTCCACCCACGCTGCCGGGCTACCCGCATGCAGGCCATCCTGTTCAATCATACTCTTC 
               
               
                 CTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAA 
               
               
                 AATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAA 
               
               
                 AATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAAT 
               
               
                 CAAAAGAATAGACCGAGATAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGT 
               
               
                 TGGGAAGGGCGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATT 
               
               
                 AAGTTGGGTAACGCCAGGGTTTTCCCAGTCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A531 Vector: SGP-gHso1-SGP-gL (SEQ ID NO: 58): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTCGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGGCCTGGCCT   
               
               
                 
                   GCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGT 
                 
               
               
                 
                   GAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAA 
                 
               
               
                 CACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTT 
               
               
                 
                   TTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTT 
                 
               
               
                 
                   CCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAA 
                 
               
               
                 
                   GGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGT 
                 
               
               
                 
                   GCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAG 
                 
               
               
                 
                   CCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTT 
                 
               
               
                 
                   TAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGAC 
                 
               
               
                 
                   CGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAG 
                 
               
               
                 
                   AGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCT 
                 
               
               
                 
                   GGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAA 
                 
               
               
                 
                   CTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTG 
                 
               
               
                 
                   CCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGA 
                 
               
               
                 
                   AGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCAT 
                 
               
               
                 
                   GATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAG 
                 
               
               
                 
                   GGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAA 
                 
               
               
                 
                   CCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCT 
                 
               
               
                 
                   GGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCA 
                 
               
               
                 
                   TACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCA 
                 
               
               
                 
                   GCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCA 
                 
               
               
                 
                   CAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCT 
                 
               
               
                 
                   GTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGC 
                 
               
               
                 
                   CCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTC 
                 
               
               
                 
                   CACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACAT 
                 
               
               
                 
                   GCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCT 
                 
               
               
                 
                   GGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGA 
                 
               
               
                 
                   CCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGA 
                 
               
               
                   AGTGACCGACGTGGTGGTGGACGCCACCGAC TGATAATCTAGAGGCCCCTATAACTCTCTACGGCTAACCTGAAT 
               
               
                 GGACTACGACATAGTCTAGTCCGCCAAG ATGTGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGCCCTGGACCCGT   
               
               
                 
                   GATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCTACAGCCGCCGA 
                 
               
               
                 
                   GAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGCGACAAGTACGA 
                 
               
               
                 
                   GAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGATGGCCCCCTGAGCCAGCTGATCCGGTACAGACCCGT 
                 
               
               
                 
                   GACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGCCTTCCTGGATACCCTGGCCCTGCTGTACAACAACCC 
                 
               
               
                 
                   CGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGACACCGCCCCCAGATGGATGACCGTGATGCGGGGCTA 
                 
               
               
                 
                   CAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTGCGTGGACGACCTGTGCAGAGGCTACGACCTGACCAG 
                 
               
               
                 
                   ACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGCCTGTTCAACGT 
                 
               
               
                 
                   GGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAGAGCCGTGCGGCTGCCTGTGTCTACAGCCGCTGCACC 
                 
               
               
                 
                   TGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGTGAAAGAGTTCTGCCTCCGGCACCAGCTGGATCCCCC 
                 
               
               
                 
                   CCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTGAACCTGCCCGC 
                 
               
               
                   CCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGA TGATAAGCGGCCGCATACAGCAGCAATTGGCAAGCTGC 
               
               
                 TTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTTCTTTTCTTTTCCGAATCGGA 
               
               
                 TTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATGGCATCTCCACC 
               
               
                 TCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGAGCCACGTTTAAA 
               
               
                 CACGTGATATCTGGCCTCATGGGCCTTCCTTTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCA 
               
               
                 TTAACATGGTCATAGCTGTTTCCTTGCGTATTGGGCGCTCTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGT 
               
               
                 CGTTCGGGTAAAGCCTGGGGTGCCTAATGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTG 
               
               
                 CTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAAC 
               
               
                 CCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCG 
               
               
                 CTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTC 
               
               
                 AGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTA 
               
               
                 TCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGG 
               
               
                 ATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGA 
               
               
                 ACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAA 
               
               
                 CAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAA 
               
               
                 GATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGA 
               
               
                 TTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAG 
               
               
                 TAAACTTGGTCTGACAGTTATTAGAAAAATTCATCCAGCAGACGATAAAACGCAATACGCTGGCTATCCGGTGCC 
               
               
                 GCAATGCCATACAGCACCAGAAAACGATCCGCCCATTCGCCGCCCAGTTCTTCCGCAATATCACGGGTGGCCAGC 
               
               
                 GCAATATCCTGATAACGATCCGCCACGCCCAGACGGCCGCAATCAATAAAGCCGCTAAAACGGCCATTTTCCACC 
               
               
                 ATAATGTTCGGCAGGCACGCATCACCATGGGTCACCACCAGATCTTCGCCATCCGGCATGCTCGCTTTCAGACGC 
               
               
                 GCAAACAGCTCTGCCGGTGCCAGGCCCTGATGTTCTTCATCCAGATCATCCTGATCCACCAGGCCCGCTTCCATA 
               
               
                 CGGGTACGCGCACGTTCAATACGATGTTTCGCCTGATGATCAAACGGACAGGTCGCCGGGTCCAGGGTATGCAGA 
               
               
                 CGACGCATGGCATCCGCCATAATGCTCACTTTTTCTGCCGGCGCCAGATGGCTAGACAGCAGATCCTGACCCGGC 
               
               
                 ACTTCGCCCAGCAGCAGCCAATCACGGCCCGCTTCGGTCACCACATCCAGCACCGCCGCACACGGAACACCGGTG 
               
               
                 GTGGCCAGCCAGCTCAGACGCGCCGCTTCATCCTGCAGCTCGTTCAGCGCACCGCTCAGATCGGTTTTCACAAAC 
               
               
                 AGCACCGGACGACCCTGCGCGCTCAGACGAAACACCGCCGCATCAGAGCAGCCAATGGTCTGCTGCGCCCAATCA 
               
               
                 TAGCCAAACAGACGTTCCACCCACGCTGCCGGGCTACCCGCATGCAGGCCATCCTGTTCAATCATACTCTTCCTT 
               
               
                 TTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAAT 
               
               
                 AAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAAT 
               
               
                 TCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAA 
               
               
                 AAGAATAGACCGAGATAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGTTGG 
               
               
                 GAAGGGCGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAG 
               
               
                 TTGGGTAACGCCAGGGTTTTCCCAGTCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A532 Vector: SGP-gHso1-2A-gL (SEQ ID NO: 59): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTCGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGGCCTGGCCT   
               
               
                 
                   GCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGT 
                 
               
               
                 
                   GAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAA 
                 
               
               
                 
                   CACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTT 
                 
               
               
                 
                   TTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTT 
                 
               
               
                 
                   CCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAA 
                 
               
               
                 
                   GGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGT 
                 
               
               
                 
                   GCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAG 
                 
               
               
                 
                   CCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTT 
                 
               
               
                 
                   TAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGAC 
                 
               
               
                 
                   CGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAG 
                 
               
               
                 
                   AGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCT 
                 
               
               
                 
                   GGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAA 
                 
               
               
                 
                   CTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTG 
                 
               
               
                 
                   CCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGA 
                 
               
               
                 
                   AGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCAT 
                 
               
               
                 
                   GATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAG 
                 
               
               
                 
                   GGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAA 
                 
               
               
                 
                   CCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCT 
                 
               
               
                 
                   GGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCA 
                 
               
               
                 
                   TACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCA 
                 
               
               
                 
                   GCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCA 
                 
               
               
                 
                   CAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCT 
                 
               
               
                 GTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGC 
               
               
                 
                   CCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTC 
                 
               
               
                 
                   CACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACAT 
                 
               
               
                 
                   GCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCT 
                 
               
               
                 
                   GGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGA 
                 
               
               
                 
                   CCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGA 
                 
               
               
                   AGTGACCGACGTGGTGGTGGACGCCACCGAC CTGTTGAATTTTGACCTTCTTAAGCTTGCGGGAGACGTCGAGTC 
               
               
                 CAACCCCGGGCCC ATGTGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGCCCTGGACCCGTGATCCTGCTGTGGTG   
               
               
                 
                   CTGCCTGCTGCTGCCTATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCTACAGCCGCCGAGAAGGTGCCAGCCGA 
                 
               
               
                 
                   GTGCCCCGAGCTGACCAGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGCGACAAGTACGAGAGCTGGCTGCGGCC 
                 
               
               
                 
                   CCTGGTCAACGTGACCGGCAGAGATGGCCCCCTGAGCCAGCTGATCCGGTACAGACCCGTGACCCCCGAGGCCGC 
                 
               
               
                 
                   CAATAGCGTGCTGCTGGACGAGGCCTTCCTGGATACCCTGGCCCTGCTGTACAACAACCCCGACCAGCTGAGAGC 
                 
               
               
                 
                   CCTGCTGACCCTGCTGTCCAGCGACACCGCCCCCAGATGGATGACCGTGATGCGGGGCTACAGCGAGTGTGGAGA 
                 
               
               
                 
                   TGGCAGCCCTGCCGTGTACACCTGCGTGGACGACCTGTGCAGAGGCTACGACCTGACCAGACTGAGCTACGGCCG 
                 
               
               
                 
                   GTCCATCTTCACAGAGCACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGCCTGTTCAACGTGGTGGTGGCCATCCG 
                 
               
               
                 
                   GAACGAGGCCACCAGAACCAACAGAGCCGTGCGGCTGCCTGTGTCTACAGCCGCTGCACCTGAGGGCATCACACT 
                 
               
               
                 
                   GTTCTACGGCCTGTACAACGCCGTGAAAGAGTTCTGCCTCCGGCACCAGCTGGATCCCCCCCTGCTGAGACACCT 
                 
               
               
                 
                   GGACAAGTACTACGCCGGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTGAACCTGCCCGCCCACAGCAGATATGG 
                 
               
               
                   CCCTCAGGCCGTGGACGCCAGA TGATAAGCGGCCGCATACAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGC 
               
               
                 GGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATAT 
               
               
                 TTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGAC 
               
               
                 CTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGAGCCACGTTTAAACACGTGATATCTGGC 
               
               
                 CTCATGGGCCTTCCTTTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAACATGGTCATAG 
               
               
                 CTGTTTCCTTGCGTATTGGGCGCTCTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGGTAAAGCC 
               
               
                 TGGGGTGCCTAATGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCAT 
               
               
                 AGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAA 
               
               
                 AGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTG 
               
               
                 TCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTC 
               
               
                 GTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGT 
               
               
                 CTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGG 
               
               
                 TATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATC 
               
               
                 TGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGT 
               
               
                 AGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTT 
               
               
                 TCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATC 
               
               
                 TTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGAC 
               
               
                 AGTTATTAGAAAAATTCATCCAGCAGACGATAAAACGCAATACGCTGGCTATCCGGTGCCGCAATGCCATACAGC 
               
               
                 ACCAGAAAACGATCCGCCCATTCGCCGCCCAGTTCTTCCGCAATATCACGGGTGGCCAGCGCAATATCCTGATAA 
               
               
                 CGATCCGCCACGCCCAGACGGCCGCAATCAATAAAGCCGCTAAAACGGCCATTTTCCACCATAATGTTCGGCAGG 
               
               
                 CACGCATCACCATGGGTCACCACCAGATCTTCGCCATCCGGCATGCTCGCTTTCAGACGCGCAAACAGCTCTGCC 
               
               
                 GGTGCCAGGCCCTGATGTTCTTCATCCAGATCATCCTGATCCACCAGGCCCGCTTCCATACGGGTACGCGCACGT 
               
               
                 TCAATACGATGTTTCGCCTGATGATCAAACGGACAGGTCGCCGGGTCCAGGGTATGCAGACGACGCATGGCATCC 
               
               
                 GCCATAATGCTCACTTTTTCTGCCGGCGCCAGATGGCTAGACAGCAGATCCTGACCCGGCACTTCGCCCAGCAGC 
               
               
                 AGCCAATCACGGCCCGCTTCGGTCACCACATCCAGCACCGCCGCACACGGAACACCGGTGGTGGCCAGCCAGCTC 
               
               
                 AGACGCGCCGCTTCATCCTGCAGCTCGTTCAGCGCACCGCTCAGATCGGTTTTCACAAACAGCACCGGACGACCC 
               
               
                 TGCGCGCTCAGACGAAACACCGCCGCATCAGAGCAGCCAATGGTCTGCTGCGCCCAATCATAGCCAAACAGACGT 
               
               
                 TCCACCCACGCTGCCGGGCTACCCGCATGCAGGCCATCCTGTTCAATCATACTCTTCCTTTTTCAATATTATTGA 
               
               
                 AGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTT 
               
               
                 CCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTT 
               
               
                 GTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGA 
               
               
                 TAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTTCGGT 
               
               
                 GCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGG 
               
               
                 GTTTTCCCAGTCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A533 Vector: SGP-gHso1-EV71-gL (SEQ ID NO: 60): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTCGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGGCCTGGCCT   
               
               
                 
                   GCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGT 
                 
               
               
                 
                   GAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAA 
                 
               
               
                 
                   CACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTT 
                 
               
               
                 
                   TTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTT 
                 
               
               
                 
                   CCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAA 
                 
               
               
                 
                   GGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGT 
                 
               
               
                 
                   GCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAG 
                 
               
               
                 
                   CCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTT 
                 
               
               
                 
                   TAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGAC 
                 
               
               
                 
                   CGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAG 
                 
               
               
                 
                   AGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCT 
                 
               
               
                 
                   GGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAA 
                 
               
               
                 
                   CTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTG 
                 
               
               
                 
                   CCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGA 
                 
               
               
                 
                   AGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCAT 
                 
               
               
                 
                   GATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAG 
                 
               
               
                 
                   GGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAA 
                 
               
               
                 
                   CCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCT 
                 
               
               
                 
                   GGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCA 
                 
               
               
                 
                   TACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCA 
                 
               
               
                 
                   GCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCA 
                 
               
               
                 
                   CAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCT 
                 
               
               
                 
                   GTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGC 
                 
               
               
                 
                   CCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTC 
                 
               
               
                 
                   CACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACAT 
                 
               
               
                 
                   GCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCT 
                 
               
               
                 
                   GGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGA 
                 
               
               
                 
                   CCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGA 
                 
               
               
                   AGTGACCGACGTGGTGGTGGACGCCACCGAC TGATAATCTAGATTAAAACAGCTGTGGGTTGTTCCCACCCACAG 
               
               
                 GGCCCACTGGGCGCTAGCACTCTGATTTTACGAAATCCTTGTGCGCCTGTTTTATATCCCTTCCCTAATTCGAAA 
               
               
                 CGTAGAAGCAATGCGCACCACTGATCAATAGTAGGCGTAACGCGCCAGTTACGTCATGATCAAGCATATCTGTTC 
               
               
                 CCCCGGACTGAGTATCAATAGACTGCTTACGCGGTTGAAGGAGAAAACGTTCGTTATCCGGCTAACTACTTCGAG 
               
               
                 AAGCCCAGTAACACCATGGAAGCTGCAGGGTGTTTCGCTCAGCACTTCCCCCGTGTAGATCAGGTCGATGAGCCA 
               
               
                 CTGCAATCCCCACAGGTGACTGTGGCAGTGGCTGCGTTGGCGGCCTGCCTATGGGGAGACCCATAGGACGCTCTA 
               
               
                 ATGTGGACATGGTGCGAAGAGCCTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTG 
               
               
                 CGGAGCACATGCCTTCAACCCAGAGGGTAGTGTGTCGTAATGGGCAACTCTGCAGCGGAACCGACTACTTTGGGT 
               
               
                 GTCCGTGTTTCTTTTTATTCTTATATTGGCTGCTTATGGTGACAATTACAGAATTGTTACCATATAGCTATTGGA 
               
               
                 TTGGCCATCCGGTGTGTAATAGAGCTGTTATATACCTATTTGTTGGCTTTGTACCACTAACTTTAAAATCTATAA 
               
               
                 CTACCCTCAACTTTATATTAACCCTCAATACAGTTGAAC ATGTGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGC   
               
               
                 
                   CCTGGACCCGTGATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCT 
                 
               
               
                 
                   ACAGCCGCCGAGAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGC 
                 
               
               
                 
                   GACAAGTACGAGAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGATGGCCCCCTGAGCCAGCTGATCCGG 
                 
               
               
                 TACAGACCCGTGACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGCCTTCCTGGATACCCTGGCCCTGCTG 
               
               
                 
                   TACAACAACCCCGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGACACCGCCCCCAGATGGATGACCGTG 
                 
               
               
                 
                   ATGCGGGGCTACAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTGCGTGGACGACCTGTGCAGAGGCTAC 
                 
               
               
                 
                   GACCTGACCAGACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGC 
                 
               
               
                 
                   CTGTTCAACGTGGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAGAGCCGTGCGGCTGCCTGTGTCTACA 
                 
               
               
                 
                   GCCGCTGCACCTGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGTGAAAGAGTTCTGCCTCCGGCACCAG 
                 
               
               
                 
                   CTGGATCCCCCCCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTG 
                 
               
               
                   AACCTGCCCGCCCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGA TGATAAGCGGCCGCATACAGCAGCAAT 
               
               
                 TGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTTCTTTTCTTT 
               
               
                 TCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATG 
               
               
                 GCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGAG 
               
               
                 CCACGTTTAAACACGTGATATCTGGCCTCATGGGCCTTCCTTTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCG 
               
               
                 TGCCAGCTGCATTAACATGGTCATAGCTGTTTCCTTGCGTATTGGGCGCTCTCCGCTTCCTCGCTCACTGACTCG 
               
               
                 CTGCGCTCGGTCGTTCGGGTAAAGCCTGGGGTGCCTAATGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA 
               
               
                 AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA 
               
               
                 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC 
               
               
                 CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT 
               
               
                 GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC 
               
               
                 GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA 
               
               
                 CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT 
               
               
                 ACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT 
               
               
                 GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG 
               
               
                 GATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT 
               
               
                 TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA 
               
               
                 GTATATATGAGTAAACTTGGTCTGACAGTTATTAGAAAAATTCATCCAGCAGACGATAAAACGCAATACGCTGGC 
               
               
                 TATCCGGTGCCGCAATGCCATACAGCACCAGAAAACGATCCGCCCATTCGCCGCCCAGTTCTTCCGCAATATCAC 
               
               
                 GGGTGGCCAGCGCAATATCCTGATAACGATCCGCCACGCCCAGACGGCCGCAATCAATAAAGCCGCTAAAACGGC 
               
               
                 CATTTTCCACCATAATGTTCGGCAGGCACGCATCACCATGGGTCACCACCAGATCTTCGCCATCCGGCATGCTCG 
               
               
                 CTTTCAGACGCGCAAACAGCTCTGCCGGTGCCAGGCCCTGATGTTCTTCATCCAGATCATCCTGATCCACCAGGC 
               
               
                 CCGCTTCCATACGGGTACGCGCACGTTCAATACGATGTTTCGCCTGATGATCAAACGGACAGGTCGCCGGGTCCA 
               
               
                 GGGTATGCAGACGACGCATGGCATCCGCCATAATGCTCACTTTTTCTGCCGGCGCCAGATGGCTAGACAGCAGAT 
               
               
                 CCTGACCCGGCACTTCGCCCAGCAGCAGCCAATCACGGCCCGCTTCGGTCACCACATCCAGCACCGCCGCACACG 
               
               
                 GAACACCGGTGGTGGCCAGCCAGCTCAGACGCGCCGCTTCATCCTGCAGCTCGTTCAGCGCACCGCTCAGATCGG 
               
               
                 TTTTCACAAACAGCACCGGACGACCCTGCGCGCTCAGACGAAACACCGCCGCATCAGAGCAGCCAATGGTCTGCT 
               
               
                 GCGCCCAATCATAGCCAAACAGACGTTCCACCCACGCTGCCGGGCTACCCGCATGCAGGCCATCCTGTTCAATCA 
               
               
                 TACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTA 
               
               
                 TTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATAT 
               
               
                 TTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC 
               
               
                 TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGC 
               
               
                 GCAACTGTTGGGAAGGGCGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCA 
               
               
                 AGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A534 Vector: SGP-gL-EV71-gH (SEQ ID NO: 61): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTCGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGTGCAGAAGGCC   
               
               
                 
                   CGACTGCGGCTTCAGCTTCAGCCCTGGACCCGTGATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTCCTC 
                 
               
               
                 
                   TGCCGCCGTGTCTGTGGCCCCTACAGCCGCCGAGAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATGCCT 
                 
               
               
                 
                   GCTGGGCGAGGTGTTCGAGGGCGACAAGTACGAGAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGATGG 
                 
               
               
                 
                   CCCCCTGAGCCAGCTGATCCGGTACAGACCCGTGACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGCCTT 
                 
               
               
                 
                   CCTGGATACCCTGGCCCTGCTGTACAACAACCCCGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGACAC 
                 
               
               
                 
                   CGCCCCCAGATGGATGACCGTGATGCGGGGCTACAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTGCGT 
                 
               
               
                 
                   GGACGACCTGTGCAGAGGCTACGACCTGACCAGACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCTGGG 
                 
               
               
                 
                   CTTCGAGCTGGTGCCCCCCAGCCTGTTCAACGTGGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAGAGC 
                 
               
               
                 
                   CGTGCGGCTGCCTGTGTCTACAGCCGCTGCACCTGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGTGAA 
                 
               
               
                 
                   AGAGTTCTGCCTCCGGCACCAGCTGGATCCCCCCCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCCCCC 
                 
               
               
                   AGAGCTGAAGCAGACCAGAGTGAACCTGCCCGCCCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGA TGATA 
               
               
                 ATCTAGATTAAAACAGCTGTGGGTTGTTCCCACCCACAGGGCCCACTGGGCGCTAGCACTCTGATTTTACGAAAT 
               
               
                 CCTTGTGCGCCTGTTTTATATCCCTTCCCTAATTCGAAACGTAGAAGCAATGCGCACCACTGATCAATAGTAGGC 
               
               
                 GTAACGCGCCAGTTACGTCATGATCAAGCATATCTGTTCCCCCGGACTGAGTATCAATAGACTGCTTACGCGGTT 
               
               
                 GAAGGAGAAAACGTTCGTTATCCGGCTAACTACTTCGAGAAGCCCAGTAACACCATGGAAGCTGCAGGGTGTTTC 
               
               
                 GCTCAGCACTTCCCCCGTGTAGATCAGGTCGATGAGCCACTGCAATCCCCACAGGTGACTGTGGCAGTGGCTGCG 
               
               
                 TTGGCGGCCTGCCTATGGGGAGACCCATAGGACGCTCTAATGTGGACATGGTGCGAAGAGCCTATTGAGCTAGTT 
               
               
                 AGTAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTGCGGAGCACATGCCTTCAACCCAGAGGGTAGTGTGTC 
               
               
                 GTAATGGGCAACTCTGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCTTTTTATTCTTATATTGGCTGCTTA 
               
               
                 TGGTGACAATTACAGAATTGTTACCATATAGCTATTGGATTGGCCATCCGGTGTGTAATAGAGCTGTTATATACC 
               
               
                 TATTTGTTGGCTTTGTACCACTAACTTTAAAATCTATAACTACCCTCAACTTTATATTAACCCTCAATACAGTTG 
               
               
                 AAC ATGAGGCCTGGCCTGCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGA   
               
               
                 
                   TACGGCGCCGAGGCCGTGAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATC 
                 
               
               
                 
                   CGGTTTCTGCGGGAGAACACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAAC 
                 
               
               
                 
                   GCCATCAGCTTCAACTTTTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGC 
                 
               
               
                 
                   CCTCTGGCCGAGCAGTTCCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACC 
                 
               
               
                 
                   TACGCCCTGGTGTCCAAGGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGC 
                 
               
               
                 
                   GAGCAGCCTACCACCGTGCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCT 
                 
               
               
                 
                   CACGGCTGGACCGAGAGCCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGAC 
                 
               
               
                 
                   GGCCACGACCTGCTGTTTAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATAC 
                 
               
               
                 
                   GTGAAGATCACCCTGACCGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATC 
                 
               
               
                 
                   TTCGGCCACCTGCCCAGAGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAG 
                 
               
               
                 
                   CACGAGCTGCTGGTGCTGGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGAC 
                 
               
               
                 
                   GCCGCCCTGGACTTCAACTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTG 
                 
               
               
                 
                   CTGAAGTCCGGACGGTGCCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTC 
                 
               
               
                 
                   GCCGCTGCCAGACAGGAAGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTG 
                 
               
               
                 
                   CAGATCCAGGAATTCATGATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCC 
                 
               
               
                 
                   GTGGATCTGGCCAAGAGGGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTAC 
                 
               
               
                 
                   ATCCTGAGCAAGCAGAACCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAG 
                 
               
               
                 
                   CTGCACAAGACCCATCTGGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTC 
                 
               
               
                 
                   CACAGCATGCTGGTGCATACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAG 
                 
               
               
                 
                   CTGTCCCACTTTACCCAGCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGC 
                 
               
               
                 
                   AGCGGCAGACGGGACCACAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCT 
                 
               
               
                 
                   GCCGCCCTGTCCATCCTGTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTG 
                 
               
               
                 
                   GGCGAGAGCTTTAGCGCCCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGC 
                 
               
               
                 
                   ATCAGCTACCCCGTGTCCACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGC 
                 
               
               
                 
                   GAGCTGACCCGGAACATGCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTC 
                 
               
               
                 
                   TGTCAGTCTGCCCTGCTGGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGAC 
                 
               
               
                 
                   GTGCTGTTCGCCCTGGACCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAG 
                 
               
               
                   AACGGCACCGTGCTGGAAGTGACCGACGTGGTGGTGGACGCCACCGAC TGATAAGCGGCCGCATACAGCAGCAAT 
               
               
                 TGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTTCTTTTCTTT 
               
               
                 TCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATG 
               
               
                 GCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGAG 
               
               
                 CCACGTTTAAACACGTGATATCTGGCCTCATGGGCCTTCCTTTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCG 
               
               
                 TGCCAGCTGCATTAACATGGTCATAGCTGTTTCCTTGCGTATTGGGCGCTCTCCGCTTCCTCGCTCACTGACTCG 
               
               
                 CTGCGCTCGGTCGTTCGGGTAAAGCCTGGGGTGCCTAATGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA 
               
               
                 AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA 
               
               
                 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC 
               
               
                 CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT 
               
               
                 GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC 
               
               
                 GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA 
               
               
                 CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT 
               
               
                 ACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT 
               
               
                 GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG 
               
               
                 GATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT 
               
               
                 TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA 
               
               
                 GTATATATGAGTAAACTTGGTCTGACAGTTATTAGAAAAATTCATCCAGCAGACGATAAAACGCAATACGCTGGC 
               
               
                 TATCCGGTGCCGCAATGCCATACAGCACCAGAAAACGATCCGCCCATTCGCCGCCCAGTTCTTCCGCAATATCAC 
               
               
                 GGGTGGCCAGCGCAATATCCTGATAACGATCCGCCACGCCCAGACGGCCGCAATCAATAAAGCCGCTAAAACGGC 
               
               
                 CATTTTCCACCATAATGTTCGGCAGGCACGCATCACCATGGGTCACCACCAGATCTTCGCCATCCGGCATGCTCG 
               
               
                 CTTTCAGACGCGCAAACAGCTCTGCCGGTGCCAGGCCCTGATGTTCTTCATCCAGATCATCCTGATCCACCAGGC 
               
               
                 CCGCTTCCATACGGGTACGCGCACGTTCAATACGATGTTTCGCCTGATGATCAAACGGACAGGTCGCCGGGTCCA 
               
               
                 GGGTATGCAGACGACGCATGGCATCCGCCATAATGCTCACTTTTTCTGCCGGCGCCAGATGGCTAGACAGCAGAT 
               
               
                 CCTGACCCGGCACTTCGCCCAGCAGCAGCCAATCACGGCCCGCTTCGGTCACCACATCCAGCACCGCCGCACACG 
               
               
                 GAACACCGGTGGTGGCCAGCCAGCTCAGACGCGCCGCTTCATCCTGCAGCTCGTTCAGCGCACCGCTCAGATCGG 
               
               
                 TTTTCACAAACAGCACCGGACGACCCTGCGCGCTCAGACGAAACACCGCCGCATCAGAGCAGCCAATGGTCTGCT 
               
               
                 GCGCCCAATCATAGCCAAACAGACGTTCCACCCACGCTGCCGGGCTACCCGCATGCAGGCCATCCTGTTCAATCA 
               
               
                 TACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTA 
               
               
                 TTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATAT 
               
               
                 TTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC 
               
               
                 TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGC 
               
               
                 GCAACTGTTGGGAAGGGCGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCA 
               
               
                 AGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A535 Vector: SGP-342-EV71-gHso1-2A-gL (SEQ ID NO: 62): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTCGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAGCTATTCCAGAAGTA 
               
               
                 GTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACA 
               
               
                 GGATGAGGATCGTTTCGCATGATTGAATAAGATGGATTGCACGTAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTA 
               
               
                 TTCGGCTATGACTGGGCACAACTGACAATCGGCTGCTCTGATGCCGCCGTGATCCGGTTGTCAGCGCAGGGGCGC 
               
               
                 CCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGAAGGACGAGGCAGCGCGGCTATCGTGG 
               
               
                 CTGGCCACGACGGGCGTTCCTTGCGCAGTCTAGACTGGCGCGCCAAACCTGCAGGTTAAAACAGCTGTGGGTTGT 
               
               
                 TCCCACCCACAGGGCCCACTGGGCGCTAGCACTCTGATTTTACGAAATCCTTGTGCGCCTGTTTTATATCCCTTC 
               
               
                 CCTAATTCGAAACGTAGAAGCAATGCGCACCACTGATCAATAGTAGGCGTAACGCGCCAGTTACGTCATGATCAA 
               
               
                 GCATATCTGTTCCCCCGGACTGAGTATCAATAGACTGCTTACGCGGTTGAAGGAGAAAACGTTCGTTATCCGGCT 
               
               
                 AACTACTTCGAGAAGCCCAGTAACACCATGGAAGCTGCAGGGTGTTTCGCTCAGCACTTCCCCCGTGTAGATCAG 
               
               
                 GTCGATGAGCCACTGCAATCCCCACAGGTGACTGTGGCAGTGGCTGCGTTGGCGGCCTGCCTATGGGGAGACCCA 
               
               
                 TAGGACGCTCTAATGTGGACATGGTGCGAAGAGCCTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGC 
               
               
                 TAATCCTAACTGCGGAGCACATGCCTTCAACCCAGAGGGTAGTGTGTCGTAATGGGCAACTCTGCAGCGGAACCG 
               
               
                 ACTACTTTGGGTGTCCGTGTTTCTTTTTATTCTTATATTGGCTGCTTATGGTGACAATTACAGAATTGTTACCAT 
               
               
                 ATAGCTATTGGATTGGCCATCCGGTGTGTAATAGAGCTGTTATATACCTATTTGTTGGCTTTGTACCACTAACTT 
               
               
                 TAAAATCTATAACTACCCTCAACTTTATATTAACCCTCAATACAGTTGAAC ATGAGGCCTGGCCTGCCCTCCTAC   
               
               
                 
                   CTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGTGAGCGAGCCC 
                 
               
               
                 
                   CTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAACACCACCCAG 
                 
               
               
                 
                   TGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTTTTTCCAGAGC 
                 
               
               
                 
                   TACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTTCCTGAACCAG 
                 
               
               
                 
                   GTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAAGGACCTGGCC 
                 
               
               
                 
                   AGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGTGCCCCCTCCC 
                 
               
               
                 
                   ATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAGCCACACCACC 
                 
               
               
                 
                   TCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTTTAGCACCGTG 
                 
               
               
                 
                   ACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGACCGAGGATTTC 
                 
               
               
                 
                   TTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAGAGTGCTGTTC 
                 
               
               
                 
                   AAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCTGGTCAAGAAG 
                 
               
               
                 
                   GACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAACTACCTGGAC 
                 
               
               
                 
                   CTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTGCCAGATGCTC 
                 
               
               
                 
                   GATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGAAGAGGCTGGC 
                 
               
               
                 
                   GCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCATGATCACCTGC 
                 
               
               
                 
                   CTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAGGGCCCTGTGG 
                 
               
               
                 
                   ACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAACCAGCAGCAC 
                 
               
               
                 
                   CTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCTGGCCAGCTTT 
                 
               
               
                 
                   CTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCATACCACCGAG 
                 
               
               
                 
                   CGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCAGCTGCTGGCC 
                 
               
               
                 
                   CACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCACAGCCTGGAA 
                 
               
               
                 
                   CGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCTGTCCACCATG 
                 
               
               
                 
                   CAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGCCCTGACCGTG 
                 
               
               
                 
                   TCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTCCACCACAGTC 
                 
               
               
                 
                   GTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACATGCACACCACA 
                 
               
               
                 
                   CACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCTGGAATACGAC 
                 
               
               
                 
                   GATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGACCCCTACAAC 
                 
               
               
                 
                   GAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGAAGTGACCGAC 
                 
               
               
                   GTGGTGGTGGACGCCACCGAC CTGTTGAATTTTGACCTTCTTAAGCTTGCGGGAGACGTCGAGTCCAACCCCGGG 
               
               
                 CCC ATGTGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGCCCTGGACCCGTGATCCTGCTGTGGTGCTGCCTGCTG   
               
               
                 
                   CTGCCTATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCTACAGCCGCCGAGAAGGTGCCAGCCGAGTGCCCCGAG 
                 
               
               
                 
                   CTGACCAGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGCGACAAGTACGAGAGCTGGCTGCGGCCCCTGGTCAAC 
                 
               
               
                 
                   GTGACCGGCAGAGATGGCCCCCTGAGCCAGCTGATCCGGTACAGACCCGTGACCCCCGAGGCCGCCAATAGCGTG 
                 
               
               
                 
                   CTGCTGGACGAGGCCTTCCTGGATACCCTGGCCCTGCTGTACAACAACCCCGACCAGCTGAGAGCCCTGCTGACC 
                 
               
               
                 
                   CTGCTGTCCAGCGACACCGCCCCCAGATGGATGACCGTGATGCGGGGCTACAGCGAGTGTGGAGATGGCAGCCCT 
                 
               
               
                 
                   GCCGTGTACACCTGCGTGGACGACCTGTGCAGAGGCTACGACCTGACCAGACTGAGCTACGGCCGGTCCATCTTC 
                 
               
               
                 
                   ACAGAGCACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGCCTGTTCAACGTGGTGGTGGCCATCCGGAACGAGGCC 
                 
               
               
                 
                   ACCAGAACCAACAGAGCCGTGCGGCTGCCTGTGTCTACAGCCGCTGCACCTGAGGGCATCACACTGTTCTACGGC 
                 
               
               
                 
                   CTGTACAACGCCGTGAAAGAGTTCTGCCTCCGGCACCAGCTGGATCCCCCCCTGCTGAGACACCTGGACAAGTAC 
                 
               
               
                 
                   TACGCCGGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTGAACCTGCCCGCCCACAGCAGATATGGCCCTCAGGCC 
                 
               
               
                   GTGGACGCCAGA TGATAAGCGGCCGCATACAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGC 
               
               
                 ATGCCGCCTTAAAATTTTTATTTTATTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAA 
               
               
                 AAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCC 
               
               
                 GAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGAGCCACGTTTAAACACGTGATATCTGGCCTCATGGGCC 
               
               
                 TTCCTTTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAACATGGTCATAGCTGTTTCCTT 
               
               
                 GCGTATTGGGCGCTCTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGGTAAAGCCTGGGGTGCCT 
               
               
                 AATGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC 
               
               
                 CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG 
               
               
                 CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC 
               
               
                 TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 
               
               
                 AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA 
               
               
                 ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG 
               
               
                 GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGC 
               
               
                 TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT 
               
               
                 TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGT 
               
               
                 CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA 
               
               
                 TCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTATTAGA 
               
               
                 AAAATTCATCCAGCAGACGATAAAACGCAATACGCTGGCTATCCGGTGCCGCAATGCCATACAGCACCAGAAAAC 
               
               
                 GATCCGCCCATTCGCCGCCCAGTTCTTCCGCAATATCACGGGTGGCCAGCGCAATATCCTGATAACGATCCGCCA 
               
               
                 CGCCCAGACGGCCGCAATCAATAAAGCCGCTAAAACGGCCATTTTCCACCATAATGTTCGGCAGGCACGCATCAC 
               
               
                 CATGGGTCACCACCAGATCTTCGCCATCCGGCATGCTCGCTTTCAGACGCGCAAACAGCTCTGCCGGTGCCAGGC 
               
               
                 CCTGATGTTCTTCATCCAGATCATCCTGATCCACCAGGCCCGCTTCCATACGGGTACGCGCACGTTCAATACGAT 
               
               
                 GTTTCGCCTGATGATCAAACGGACAGGTCGCCGGGTCCAGGGTATGCAGACGACGCATGGCATCCGCCATAATGC 
               
               
                 TCACTTTTTCTGCCGGCGCCAGATGGCTAGACAGCAGATCCTGACCCGGCACTTCGCCCAGCAGCAGCCAATCAC 
               
               
                 GGCCCGCTTCGGTCACCACATCCAGCACCGCCGCACACGGAACACCGGTGGTGGCCAGCCAGCTCAGACGCGCCG 
               
               
                 CTTCATCCTGCAGCTCGTTCAGCGCACCGCTCAGATCGGTTTTCACAAACAGCACCGGACGACCCTGCGCGCTCA 
               
               
                 GACGAAACACCGCCGCATCAGAGCAGCCAATGGTCTGCTGCGCCCAATCATAGCCAAACAGACGTTCCACCCACG 
               
               
                 CTGCCGGGCTACCCGCATGCAGGCCATCCTGTTCAATCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATC 
               
               
                 AGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACAT 
               
               
                 TTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAG 
               
               
                 CTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAG 
               
               
                 TGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTTCGGTGCGGGCCTCT 
               
               
                 TCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAG 
               
               
                 TCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A536 Vector: SGP-342-EV71-gHso1-EMCV-gL (SEQ ID NO: 63): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTCGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAGCTATTCCAGAAGTA 
               
               
                 GTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACA 
               
               
                 GGATGAGGATCGTTTCGCATGATTGAATAAGATGGATTGCACGTAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTA 
               
               
                 TTCGGCTATGACTGGGCACAACTGACAATCGGCTGCTCTGATGCCGCCGTGATCCGGTTGTCAGCGCAGGGGCGC 
               
               
                 CCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGAAGGACGAGGCAGCGCGGCTATCGTGG 
               
               
                 CTGGCCACGACGGGCGTTCCTTGCGCAGTCTAGACTGGCGCGCCAAACCTGCAGGTTAAAACAGCTGTGGGTTGT 
               
               
                 TCCCACCCACAGGGCCCACTGGGCGCTAGCACTCTGATTTTACGAAATCCTTGTGCGCCTGTTTTATATCCCTTC 
               
               
                 CCTAATTCGAAACGTAGAAGCAATGCGCACCACTGATCAATAGTAGGCGTAACGCGCCAGTTACGTCATGATCAA 
               
               
                 GCATATCTGTTCCCCCGGACTGAGTATCAATAGACTGCTTACGCGGTTGAAGGAGAAAACGTTCGTTATCCGGCT 
               
               
                 AACTACTTCGAGAAGCCCAGTAACACCATGGAAGCTGCAGGGTGTTTCGCTCAGCACTTCCCCCGTGTAGATCAG 
               
               
                 GTCGATGAGCCACTGCAATCCCCACAGGTGACTGTGGCAGTGGCTGCGTTGGCGGCCTGCCTATGGGGAGACCCA 
               
               
                 TAGGACGCTCTAATGTGGACATGGTGCGAAGAGCCTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGC 
               
               
                 TAATCCTAACTGCGGAGCACATGCCTTCAACCCAGAGGGTAGTGTGTCGTAATGGGCAACTCTGCAGCGGAACCG 
               
               
                 ACTACTTTGGGTGTCCGTGTTTCTTTTTATTCTTATATTGGCTGCTTATGGTGACAATTACAGAATTGTTACCAT 
               
               
                 ATAGCTATTGGATTGGCCATCCGGTGTGTAATAGAGCTGTTATATACCTATTTGTTGGCTTTGTACCACTAACTT 
               
               
                 TAAAATCTATAACTACCCTCAACTTTATATTAACCCTCAATACAGTTGAAC ATGAGGCCTGGCCTGCCCTCCTAC   
               
               
                 
                   CTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGTGAGCGAGCCC 
                 
               
               
                 
                   CTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAACACCACCCAG 
                 
               
               
                 
                   TGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTTTTTCCAGAGC 
                 
               
               
                 
                   TACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTTCCTGAACCAG 
                 
               
               
                 
                   GTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAAGGACCTGGCC 
                 
               
               
                 
                   AGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGTGCCCCCTCCC 
                 
               
               
                 
                   ATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAGCCACACCACC 
                 
               
               
                 
                   TCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTTTAGCACCGTG 
                 
               
               
                 
                   ACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGACCGAGGATTTC 
                 
               
               
                 
                   TTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAGAGTGCTGTTC 
                 
               
               
                 
                   AAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCTGGTCAAGAAG 
                 
               
               
                 
                   GACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAACTACCTGGAC 
                 
               
               
                 
                   CTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTGCCAGATGCTC 
                 
               
               
                 
                   GATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGAAGAGGCTGGC 
                 
               
               
                 
                   GCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCATGATCACCTGC 
                 
               
               
                 
                   CTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAGGGCCCTGTGG 
                 
               
               
                 
                   ACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAACCAGCAGCAC 
                 
               
               
                 
                   CTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCTGGCCAGCTTT 
                 
               
               
                 
                   CTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCATACCACCGAG 
                 
               
               
                 
                   CGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCAGCTGCTGGCC 
                 
               
               
                 
                   CACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCACAGCCTGGAA 
                 
               
               
                 
                   CGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCTGTCCACCATG 
                 
               
               
                 
                   CAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGCCCTGACCGTG 
                 
               
               
                 
                   TCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTCCACCACAGTC 
                 
               
               
                 
                   GTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACATGCACACCACA 
                 
               
               
                 
                   CACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCTGGAATACGAC 
                 
               
               
                 
                   GATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGACCCCTACAAC 
                 
               
               
                 
                   GAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGAAGTGACCGAC 
                 
               
               
                   GTGGTGGTGGACGCCACCGAC TGATAACGCCGGCGCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAG 
               
               
                 GCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTG 
               
               
                 GCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCG 
               
               
                 TGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACC 
               
               
                 CCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCC 
               
               
                 AGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGA 
               
               
                 AGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGT 
               
               
                 CGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATAATAAT ATG   
               
               
                 
                   TGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGCCCTGGACCCGTGATCCTGCTGTGGTGCTGCCTGCTGCTGCCT 
                 
               
               
                 
                   ATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCTACAGCCGCCGAGAAGGTGCCAGCCGAGTGCCCCGAGCTGACC 
                 
               
               
                 
                   AGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGCGACAAGTACGAGAGCTGGCTGCGGCCCCTGGTCAACGTGACC 
                 
               
               
                 
                   GGCAGAGATGGCCCCCTGAGCCAGCTGATCCGGTACAGACCCGTGACCCCCGAGGCCGCCAATAGCGTGCTGCTG 
                 
               
               
                 
                   GACGAGGCCTTCCTGGATACCCTGGCCCTGCTGTACAACAACCCCGACCAGCTGAGAGCCCTGCTGACCCTGCTG 
                 
               
               
                 
                   TCCAGCGACACCGCCCCCAGATGGATGACCGTGATGCGGGGCTACAGCGAGTGTGGAGATGGCAGCCCTGCCGTG 
                 
               
               
                 
                   TACACCTGCGTGGACGACCTGTGCAGAGGCTACGACCTGACCAGACTGAGCTACGGCCGGTCCATCTTCACAGAG 
                 
               
               
                 
                   CACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGCCTGTTCAACGTGGTGGTGGCCATCCGGAACGAGGCCACCAGA 
                 
               
               
                 
                   ACCAACAGAGCCGTGCGGCTGCCTGTGTCTACAGCCGCTGCACCTGAGGGCATCACACTGTTCTACGGCCTGTAC 
                 
               
               
                 
                   AACGCCGTGAAAGAGTTCTGCCTCCGGCACCAGCTGGATCCCCCCCTGCTGAGACACCTGGACAAGTACTACGCC 
                 
               
               
                 
                   GGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTGAACCTGCCCGCCCACAGCAGATATGGCCCTCAGGCCGTGGAC 
                 
               
               
                   GCCAGA TGATAAGCGGCCGCATACAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCG 
               
               
                 CCTTAAAATTTTTATTTTATTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAA 
               
               
                 AAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGA 
               
               
                 GGACGCACGTCCACTCGGATGGCTAAGGGAGAGCCACGTTTAAACACGTGATATCTGGCCTCATGGGCCTTCCTT 
               
               
                 TCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAACATGGTCATAGCTGTTTCCTTGCGTAT 
               
               
                 TGGGCGCTCTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGGTAAAGCCTGGGGTGCCTAATGAG 
               
               
                 CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG 
               
               
                 ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC 
               
               
                 CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT 
               
               
                 CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG 
               
               
                 GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG 
               
               
                 TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA 
               
               
                 CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGC 
               
               
                 CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG 
               
               
                 TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG 
               
               
                 CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT 
               
               
                 TAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTATTAGAAAAATT 
               
               
                 CATCCAGCAGACGATAAAACGCAATACGCTGGCTATCCGGTGCCGCAATGCCATACAGCACCAGAAAACGATCCG 
               
               
                 CCCATTCGCCGCCCAGTTCTTCCGCAATATCACGGGTGGCCAGCGCAATATCCTGATAACGATCCGCCACGCCCA 
               
               
                 GACGGCCGCAATCAATAAAGCCGCTAAAACGGCCATTTTCCACCATAATGTTCGGCAGGCACGCATCACCATGGG 
               
               
                 TCACCACCAGATCTTCGCCATCCGGCATGCTCGCTTTCAGACGCGCAAACAGCTCTGCCGGTGCCAGGCCCTGAT 
               
               
                 GTTCTTCATCCAGATCATCCTGATCCACCAGGCCCGCTTCCATACGGGTACGCGCACGTTCAATACGATGTTTCG 
               
               
                 CCTGATGATCAAACGGACAGGTCGCCGGGTCCAGGGTATGCAGACGACGCATGGCATCCGCCATAATGCTCACTT 
               
               
                 TTTCTGCCGGCGCCAGATGGCTAGACAGCAGATCCTGACCCGGCACTTCGCCCAGCAGCAGCCAATCACGGCCCG 
               
               
                 CTTCGGTCACCACATCCAGCACCGCCGCACACGGAACACCGGTGGTGGCCAGCCAGCTCAGACGCGCCGCTTCAT 
               
               
                 CCTGCAGCTCGTTCAGCGCACCGCTCAGATCGGTTTTCACAAACAGCACCGGACGACCCTGCGCGCTCAGACGAA 
               
               
                 ACACCGCCGCATCAGAGCAGCCAATGGTCTGCTGCGCCCAATCATAGCCAAACAGACGTTCCACCCACGCTGCCG 
               
               
                 GGCTACCCGCATGCAGGCCATCCTGTTCAATCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT 
               
               
                 ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                 GAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATT 
               
               
                 TTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGGCCG 
               
               
                 CTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTTCGGTGCGGGCCTCTTCGCTA 
               
               
                 TTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACAC 
               
               
                 GCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A537 Vector: SGP-342-EV71-gL-EMCV-gHso1 (SEQ ID NO: 64): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTCGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAGCTATTCCAGAAGTA 
               
               
                 GTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACA 
               
               
                 GGATGAGGATCGTTTCGCATGATTGAATAAGATGGATTGCACGTAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTA 
               
               
                 TTCGGCTATGACTGGGCACAACTGACAATCGGCTGCTCTGATGCCGCCGTGATCCGGTTGTCAGCGCAGGGGCGC 
               
               
                 CCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGAAGGACGAGGCAGCGCGGCTATCGTGG 
               
               
                 CTGGCCACGACGGGCGTTCCTTGCGCAGTCTAGACTGGCGCGCCAAACCTGCAGGTTAAAACAGCTGTGGGTTGT 
               
               
                 TCCCACCCACAGGGCCCACTGGGCGCTAGCACTCTGATTTTACGAAATCCTTGTGCGCCTGTTTTATATCCCTTC 
               
               
                 CCTAATTCGAAACGTAGAAGCAATGCGCACCACTGATCAATAGTAGGCGTAACGCGCCAGTTACGTCATGATCAA 
               
               
                 GCATATCTGTTCCCCCGGACTGAGTATCAATAGACTGCTTACGCGGTTGAAGGAGAAAACGTTCGTTATCCGGCT 
               
               
                 AACTACTTCGAGAAGCCCAGTAACACCATGGAAGCTGCAGGGTGTTTCGCTCAGCACTTCCCCCGTGTAGATCAG 
               
               
                 GTCGATGAGCCACTGCAATCCCCACAGGTGACTGTGGCAGTGGCTGCGTTGGCGGCCTGCCTATGGGGAGACCCA 
               
               
                 TAGGACGCTCTAATGTGGACATGGTGCGAAGAGCCTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGC 
               
               
                 TAATCCTAACTGCGGAGCACATGCCTTCAACCCAGAGGGTAGTGTGTCGTAATGGGCAACTCTGCAGCGGAACCG 
               
               
                 ACTACTTTGGGTGTCCGTGTTTCTTTTTATTCTTATATTGGCTGCTTATGGTGACAATTACAGAATTGTTACCAT 
               
               
                 ATAGCTATTGGATTGGCCATCCGGTGTGTAATAGAGCTGTTATATACCTATTTGTTGGCTTTGTACCACTAACTT 
               
               
                 TAAAATCTATAACTACCCTCAACTTTATATTAACCCTCAATACAGTTGAAC ATGTGCAGAAGGCCCGACTGCGGC   
               
               
                 
                   TTCAGCTTCAGCCCTGGACCCGTGATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTCCTCTGCCGCCGTG 
                 
               
               
                 
                   TCTGTGGCCCCTACAGCCGCCGAGAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATGCCTGCTGGGCGAG 
                 
               
               
                 
                   GTGTTCGAGGGCGACAAGTACGAGAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGATGGCCCCCTGAGC 
                 
               
               
                 
                   CAGCTGATCCGGTACAGACCCGTGACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGCCTTCCTGGATACC 
                 
               
               
                 
                   CTGGCCCTGCTGTACAACAACCCCGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGACACCGCCCCCAGA 
                 
               
               
                 
                   TGGATGACCGTGATGCGGGGCTACAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTGCGTGGACGACCTG 
                 
               
               
                 
                   TGCAGAGGCTACGACCTGACCAGACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCTGGGCTTCGAGCTG 
                 
               
               
                 
                   GTGCCCCCCAGCCTGTTCAACGTGGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAGAGCCGTGCGGCTG 
                 
               
               
                 
                   CCTGTGTCTACAGCCGCTGCACCTGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGTGAAAGAGTTCTGC 
                 
               
               
                 
                   CTCCGGCACCAGCTGGATCCCCCCCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCCCCCAGAGCTGAAG 
                 
               
               
                   CAGACCAGAGTGAACCTGCCCGCCCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGA TGATAACGCCGGCGC 
               
               
                 CCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCA 
               
               
                 TATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTT 
               
               
                 CCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGAC 
               
               
                 AAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGC 
               
               
                 CACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAG 
               
               
                 TCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTG 
               
               
                 ATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACG 
               
               
                 GGGACGTGGTTTTCCTTTGAAAAACACGATAATAAT ATGAGGCCTGGCCTGCCCTCCTACCTGATCATCCTGGCC   
               
               
                 
                   GTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGTGAGCGAGCCCCTGGACAAGGCTTTC 
                 
               
               
                 
                   CACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAACACCACCCAGTGCACCTACAACAGC 
                 
               
               
                 
                   AGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTTTTTCCAGAGCTACAACCAGTACTAC 
                 
               
               
                 
                   GTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTTCCTGAACCAGGTGGACCTGACCGAG 
                 
               
               
                 
                   ACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAAGGACCTGGCCAGCTACCGGTCCTTT 
                 
               
               
                 
                   AGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGTGCCCCCTCCCATCGACCTGAGCATC 
                 
               
               
                 
                   CCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAGCCACACCACCTCCGGCCTGCACAGA 
                 
               
               
                 
                   CCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTTTAGCACCGTGACCCCCTGCCTGCAC 
                 
               
               
                 
                   CAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGACCGAGGATTTCTTCGTGGTCACCGTG 
                 
               
               
                 
                   TCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAGAGTGCTGTTCAAGGCCCCCTACCAG 
                 
               
               
                 
                   CGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCTGGTCAAGAAGGACCAGCTGAACCGG 
                 
               
               
                 
                   CACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAACTACCTGGACCTGAGCGCCCTGCTG 
                 
               
               
                 
                   AGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTGCCAGATGCTCGATCGGCGGACCGTG 
                 
               
               
                 
                   GAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGAAGAGGCTGGCGCCCAGGTGTCAGTG 
                 
               
               
                 
                   CCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCATGATCACCTGCCTGAGCCAGACCCCC 
                 
               
               
                 
                   CCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAGGGCCCTGTGGACCCCCAACCAGATC 
                 
               
               
                 
                   ACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAACCAGCAGCACCTGATCCCCCAGTGG 
                 
               
               
                 
                   GCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCTGGCCAGCTTTCTGAGCGCCTTCGCC 
                 
               
               
                 
                   AGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCATACCACCGAGCGGCGGGAGATCTTC 
                 
               
               
                 
                   ATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCAGCTGCTGGCCCACCCTCACCACGAG 
                 
               
               
                 
                   TACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCACAGCCTGGAACGGCTGACCAGACTG 
                 
               
               
                 
                   TTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCTGTCCACCATGCAGCCCAGCACCCTG 
                 
               
               
                 
                   GAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGCCCTGACCGTGTCCGAGCACGTGTCC 
                 
               
               
                 
                   TACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTCCACCACAGTCGTGGGCCAGAGCCTG 
                 
               
               
                 
                   ATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACATGCACACCACACACAGCATCACCGTG 
                 
               
               
                 
                   GCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCTGGAATACGACGATACCCAGGGCGTG 
                 
               
               
                 
                   ATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGACCCCTACAACGAGGTGGTGGTGTCC 
                 
               
               
                 
                   AGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGAAGTGACCGACGTGGTGGTGGACGCC 
                 
               
               
                   ACCGAC TGATAAGCGGCCGCATACAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCG 
               
               
                 CCTTAAAATTTTTATTTTATTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAA 
               
               
                 AAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGA 
               
               
                 GGACGCACGTCCACTCGGATGGCTAAGGGAGAGCCACGTTTAAACACGTGATATCTGGCCTCATGGGCCTTCCTT 
               
               
                 TCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAACATGGTCATAGCTGTTTCCTTGCGTAT 
               
               
                 TGGGCGCTCTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGGTAAAGCCTGGGGTGCCTAATGAG 
               
               
                 CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG 
               
               
                 ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC 
               
               
                 CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT 
               
               
                 CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG 
               
               
                 GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG 
               
               
                 TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA 
               
               
                 CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGC 
               
               
                 CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG 
               
               
                 TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG 
               
               
                 CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT 
               
               
                 TAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTATTAGAAAAATT 
               
               
                 CATCCAGCAGACGATAAAACGCAATACGCTGGCTATCCGGTGCCGCAATGCCATACAGCACCAGAAAACGATCCG 
               
               
                 CCCATTCGCCGCCCAGTTCTTCCGCAATATCACGGGTGGCCAGCGCAATATCCTGATAACGATCCGCCACGCCCA 
               
               
                 GACGGCCGCAATCAATAAAGCCGCTAAAACGGCCATTTTCCACCATAATGTTCGGCAGGCACGCATCACCATGGG 
               
               
                 TCACCACCAGATCTTCGCCATCCGGCATGCTCGCTTTCAGACGCGCAAACAGCTCTGCCGGTGCCAGGCCCTGAT 
               
               
                 GTTCTTCATCCAGATCATCCTGATCCACCAGGCCCGCTTCCATACGGGTACGCGCACGTTCAATACGATGTTTCG 
               
               
                 CCTGATGATCAAACGGACAGGTCGCCGGGTCCAGGGTATGCAGACGACGCATGGCATCCGCCATAATGCTCACTT 
               
               
                 TTTCTGCCGGCGCCAGATGGCTAGACAGCAGATCCTGACCCGGCACTTCGCCCAGCAGCAGCCAATCACGGCCCG 
               
               
                 CTTCGGTCACCACATCCAGCACCGCCGCACACGGAACACCGGTGGTGGCCAGCCAGCTCAGACGCGCCGCTTCAT 
               
               
                 CCTGCAGCTCGTTCAGCGCACCGCTCAGATCGGTTTTCACAAACAGCACCGGACGACCCTGCGCGCTCAGACGAA 
               
               
                 ACACCGCCGCATCAGAGCAGCCAATGGTCTGCTGCGCCCAATCATAGCCAAACAGACGTTCCACCCACGCTGCCG 
               
               
                 GGCTACCCGCATGCAGGCCATCCTGTTCAATCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT 
               
               
                 ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                 GAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATT 
               
               
                 TTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGGCCG 
               
               
                 CTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTTCGGTGCGGGCCTCTTCGCTA 
               
               
                 TTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACAC 
               
               
                 GCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A554 Vector: SGP-gH-SGP-gL-SGP-UL128-SGP-UL130-SGP-UL131 (SEQ ID NO: 65) 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTAGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGGCCTGGCCT   
               
               
                 GCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGT 
               
               
                 
                   GAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAA 
                 
               
               
                 
                   CACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTT 
                 
               
               
                 
                   TTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTT 
                 
               
               
                 
                   CCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAA 
                 
               
               
                 
                   GGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGT 
                 
               
               
                 
                   GCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAG 
                 
               
               
                 
                   CCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTT 
                 
               
               
                 
                   TAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGAC 
                 
               
               
                 
                   CGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAG 
                 
               
               
                 
                   AGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCT 
                 
               
               
                 
                   GGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAA 
                 
               
               
                 
                   CTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTG 
                 
               
               
                 
                   CCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGA 
                 
               
               
                 
                   AGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCAT 
                 
               
               
                 
                   GATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAG 
                 
               
               
                 
                   GGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAA 
                 
               
               
                 
                   CCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCT 
                 
               
               
                 
                   GGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCA 
                 
               
               
                 
                   TACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCA 
                 
               
               
                 
                   GCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCA 
                 
               
               
                 
                   CAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCT 
                 
               
               
                 
                   GTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGC 
                 
               
               
                 
                   CCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTC 
                 
               
               
                 
                   CACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACAT 
                 
               
               
                 
                   GCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCT 
                 
               
               
                 
                   GGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGA 
                 
               
               
                 
                   CCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGA 
                 
               
               
                 
                   AGTGACCGACGTGGTGGTGGACGCCACCGACAGCAGACTGCTGATGATGAGCGTGTACGCCCTGAGCGCCATCAT 
                 
               
               
                   CGGCATCTACCTGCTGTACCGGATGCTGAAAACCTGC TGATAATCTAGAGGCCCCTATAACTCTCTACGGCTAAC 
               
               
                 CTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGTGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGCCCTGG   
               
               
                 
                   ACCCGTGATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCTACAGC 
                 
               
               
                 
                   CGCCGAGAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGCGACAA 
                 
               
               
                 
                   GTACGAGAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGATGGCCCCCTGAGCCAGCTGATCCGGTACAG 
                 
               
               
                 
                   ACCCGTGACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGCCTTCCTGGATACCCTGGCCCTGCTGTACAA 
                 
               
               
                 
                   CAACCCCGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGACACCGCCCCCAGATGGATGACCGTGATGCG 
                 
               
               
                 
                   GGGCTACAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTGCGTGGACGACCTGTGCAGAGGCTACGACCT 
                 
               
               
                 
                   GACCAGACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGCCTGTT 
                 
               
               
                 
                   CAACGTGGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAGAGCCGTGCGGCTGCCTGTGTCTACAGCCGC 
                 
               
               
                 
                   TGCACCTGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGTGAAAGAGTTCTGCCTCCGGCACCAGCTGGA 
                 
               
               
                 
                   TCCCCCCCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTGAACCT 
                 
               
               
                   GCCCGCCCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGA TGATAACGCCGGCGGCCCCTATAACTCTCTAC 
               
               
                 GGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGCCCCAAGGACCTGACCCCCTTCCTGACAA   
               
               
                 
                   CCCTGTGGCTGCTCCTGGGCCATAGCAGAGTGCCTAGAGTGCGGGCCGAGGAATGCTGCGAGTTCATCAACGTGA 
                 
               
               
                 
                   ACCACCCCCCCGAGCGGTGCTACGACTTCAAGATGTGCAACCGGTTCACCGTGGCCCTGAGATGCCCCGACGGCG 
                 
               
               
                 
                   AAGTGTGCTACAGCCCCGAGAAAACCGCCGAGATCCGGGGCATCGTGACCACCATGACCCACAGCCTGACCCGGC 
                 
               
               
                 
                   AGGTGGTGCACAACAAGCTGACCAGCTGCAACTACAACCCCCTGTACCTGGAAGCCGACGGCCGGATCAGATGCG 
                 
               
               
                 
                   GCAAAGTGAACGACAAGGCCCAGTACCTGCTGGGAGCCGCCGGAAGCGTGCCCTACCGGTGGATCAACCTGGAAT 
                 
               
               
                 
                   ACGACAAGATCACCCGGATCGTGGGCCTGGACCAGTACCTGGAAAGCGTGAAGAAGCACAAGCGGCTGGACGTGT 
                 
               
               
                   GCAGAGCCAAGATGGGCTACATGCTGCAG TGATAAGGCGCGCCGCCCCTATAACTCTCTACGGCTAACCTGAATG 
               
               
                 GACTACGACATAGTCTAGTCCGCCAAG ATGCTGCGGCTGCTGCTGAGACACCACTTCCACTGCCTGCTGCTGTGT   
               
               
                 
                   GCCGTGTGGGCCACCCCTTGTCTGGCCAGCCCTTGGAGCACCCTGACCGCCAACCAGAACCCTAGCCCCCCTTGG 
                 
               
               
                 
                   TCCAAGCTGACCTACAGCAAGCCCCACGACGCCGCCACCTTCTACTGCCCCTTTCTGTACCCCAGCCCTCCCAGA 
                 
               
               
                 
                   AGCCCCCTGCAGTTCAGCGGCTTCCAGAGAGTGTCCACCGGCCCTGAGTGCCGGAACGAGACACTGTACCTGCTG 
                 
               
               
                 
                   TACAACCGGGAGGGCCAGACACTGGTGGAGCGGAGCAGCACCTGGGTGAAAAAAGTGATCTGGTATCTGAGCGGC 
                 
               
               
                 
                   CGGAACCAGACCATCCTGCAGCGGATGCCCAGAACCGCCAGCAAGCCCAGCGACGGCAACGTGCAGATCAGCGTG 
                 
               
               
                 
                   GAGGACGCCAAAATCTTCGGAGCCCACATGGTGCCCAAGCAGACCAAGCTGCTGAGATTCGTGGTCAACGACGGC 
                 
               
               
                 
                   ACCAGATATCAGATGTGCGTGATGAAGCTGGAAAGCTGGGCCCACGTGTTCCGGGACTACTCCGTGAGCTTCCAG 
                 
               
               
                   GTCCGGCTGACCTTCACCGAGGCCAACAACCAGACCTACACCTTCTGCACCCACCCCAACCTGATCGTG TGATAA 
               
               
                 GCGGCCGCGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGCGGCT   
               
               
                 
                   GTGCAGAGTGTGGCTGTCCGTGTGCCTGTGTGCCGTGGTGCTGGGCCAGTGCCAGAGAGAGACAGCCGAGAAGAA 
                 
               
               
                 
                   CGACTACTACCGGGTGCCCCACTACTGGGATGCCTGCAGCAGAGCCCTGCCCGACCAGACCCGGTACAAATACGT 
                 
               
               
                 
                   GGAGCAGCTCGTGGACCTGACCCTGAACTACCACTACGACGCCAGCCACGGCCTGGACAACTTCGACGTGCTGAA 
                 
               
               
                 
                   GCGGATCAACGTGACCGAGGTGTCCCTGCTGATCAGCGACTTCCGGCGGCAGAACAGAAGAGGCGGCACCAACAA 
                 
               
               
                 
                   GCGGACCACCTTCAACGCCGCTGGCTCTCTGGCCCCTCACGCCAGATCCCTGGAATTCAGCGTGCGGCTGTTCGC 
                 
               
               
                 CAACTGATAACGTTGCATCCTGCAGGATACAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGC 
               
               
                 ATGCCGCCTTAAAATTTTTATTTTATTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAA 
               
               
                 AAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCC 
               
               
                 GAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGAGCCACGTTTAAACGCTAGAGCAAGACGTTTCCCGTTG 
               
               
                 AATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTT 
               
               
                 ATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTT 
               
               
                 GAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTG 
               
               
                 GTCCACCTACAACAAAGCTCTCATCAACCGTGGCTCCCTCACTTTCTGGCTGGATGATGGGGCGATTCAGGCCTG 
               
               
                 GTATGAGTCAGCAACACCTTCTTCACGAGGCAGACCTCAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGC 
               
               
                 ACTGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAGCAGAATATGT 
               
               
                 GATACAGGATATATTCCGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGG 
               
               
                 CTTACGAACGGGGCGGAGATTTCCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGGCCGCGGCAAA 
               
               
                 GCCGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGTGGTGGCGAAACCC 
               
               
                 GACAGGACTATAAAGATACCAGGCGTTTCCCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCGGTTT 
               
               
                 ACCGGTGTCATTCCGCTGTTATGGCCGCGTTTGTCTCATTCCACGCCTGACACTCAGTTCCGGGTAGGCAGTTCG 
               
               
                 CTCCAAGCTGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGA 
               
               
                 GTCCAACCCGGAAAGACATGCAAAAGCACCACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGA 
               
               
                 AGTCATGCGCCGGTTAAGGCTAAACTGAAAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGT 
               
               
                 TCAAAGAGTTGGTAGCTCAGAGAACCTTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGA 
               
               
                 TTACGCGCAGACCAAAACGATCTCAAGAAGATCATCTTATTAAGGGGTCTGACGCTCAGTGGAACGAAAACTCAC 
               
               
                 GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTA 
               
               
                 AATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTATTAGAAAAATTCATCCAGCAGACGATAAAACG 
               
               
                 CAATACGCTGGCTATCCGGTGCCGCAATGCCATACAGCACCAGAAAACGATCCGCCCATTCGCCGCCCAGTTCTT 
               
               
                 CCGCAATATCACGGGTGGCCAGCGCAATATCCTGATAACGATCCGCCACGCCCAGACGGCCGCAATCAATAAAGC 
               
               
                 CGCTAAAACGGCCATTTTCCACCATAATGTTCGGCAGGCACGCATCACCATGGGTCACCACCAGATCTTCGCCAT 
               
               
                 CCGGCATGCTCGCTTTCAGACGCGCAAACAGCTCTGCCGGTGCCAGGCCCTGATGTTCTTCATCCAGATCATCCT 
               
               
                 GATCCACCAGGCCCGCTTCCATACGGGTACGCGCACGTTCAATACGATGTTTCGCCTGATGATCAAACGGACAGG 
               
               
                 TCGCCGGGTCCAGGGTATGCAGACGACGCATGGCATCCGCCATAATGCTCACTTTTTCTGCCGGCGCCAGATGGC 
               
               
                 TAGACAGCAGATCCTGACCCGGCACTTCGCCCAGCAGCAGCCAATCACGGCCCGCTTCGGTCACCACATCCAGCA 
               
               
                 CCGCCGCACACGGAACACCGGTGGTGGCCAGCCAGCTCAGACGCGCCGCTTCATCCTGCAGCTCGTTCAGCGCAC 
               
               
                 CGCTCAGATCGGTTTTCACAAACAGCACCGGACGACCCTGCGCGCTCAGACGAAACACCGCCGCATCAGAGCAGC 
               
               
                 CAATGGTCTGCTGCGCCCAATCATAGCCAAACAGACGTTCCACCCACGCTGCCGGGCTACCCGCATGCAGGCCAT 
               
               
                 CCTGTTCAATCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACA 
               
               
                 TATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGT 
               
               
                 AAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAAT 
               
               
                 CGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGC 
               
               
                 CATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGG 
               
               
                 GGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A555 Vector: SGP-gHso1-SGP-gL-SGP-UL128-SGP-UL130-SGP-UL131 (SEQ ID NO: 66): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTAGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGGCCTGGCCT   
               
               
                 
                   GCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGT 
                 
               
               
                 
                   GAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAA 
                 
               
               
                 
                   CACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTT 
                 
               
               
                 
                   TTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTT 
                 
               
               
                 
                   CCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAA 
                 
               
               
                 
                   GGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGT 
                 
               
               
                 
                   GCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAG 
                 
               
               
                 
                   CCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTT 
                 
               
               
                 
                   TAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGAC 
                 
               
               
                 
                   CGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAG 
                 
               
               
                 
                   AGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCT 
                 
               
               
                 
                   GGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAA 
                 
               
               
                 
                   CTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTG 
                 
               
               
                 
                   CCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGA 
                 
               
               
                 
                   AGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCAT 
                 
               
               
                 
                   GATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAG 
                 
               
               
                 
                   GGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAA 
                 
               
               
                 
                   CCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCT 
                 
               
               
                 
                   GGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCA 
                 
               
               
                 
                   TACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCA 
                 
               
               
                 
                   GCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCA 
                 
               
               
                 
                   CAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCT 
                 
               
               
                 
                   GTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGC 
                 
               
               
                 
                   CCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTC 
                 
               
               
                 
                   CACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACAT 
                 
               
               
                 
                   GCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCT 
                 
               
               
                 
                   GGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGA 
                 
               
               
                 
                   CCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGA 
                 
               
               
                   AGTGACCGACGTGGTGGTGGACGCCACCGAC TGATAATCTAGAGGCCCCTATAACTCTCTACGGCTAACCTGAAT 
               
               
                 GGACTACGACATAGTCTAGTCCGCCAAG ATGTGCAGAAGGCCCGACTGCGGCTTCAGCTTCAGCCCTGGACCCGT   
               
               
                 
                   GATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTCCTCTGCCGCCGTGTCTGTGGCCCCTACAGCCGCCGA 
                 
               
               
                 
                   GAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATGCCTGCTGGGCGAGGTGTTCGAGGGCGACAAGTACGA 
                 
               
               
                 
                   GAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGATGGCCCCCTGAGCCAGCTGATCCGGTACAGACCCGT 
                 
               
               
                 
                   GACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGCCTTCCTGGATACCCTGGCCCTGCTGTACAACAACCC 
                 
               
               
                 
                   CGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGACACCGCCCCCAGATGGATGACCGTGATGCGGGGCTA 
                 
               
               
                 
                   CAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTGCGTGGACGACCTGTGCAGAGGCTACGACCTGACCAG 
                 
               
               
                 
                   ACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCTGGGCTTCGAGCTGGTGCCCCCCAGCCTGTTCAACGT 
                 
               
               
                 
                   GGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAGAGCCGTGCGGCTGCCTGTGTCTACAGCCGCTGCACC 
                 
               
               
                 
                   TGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGTGAAAGAGTTCTGCCTCCGGCACCAGCTGGATCCCCC 
                 
               
               
                 
                   CCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCCCCCAGAGCTGAAGCAGACCAGAGTGAACCTGCCCGC 
                 
               
               
                   CCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGAT GATAACGCCGGCGGCCCCTATAACTCTCTACGGCTAA 
               
               
                 CCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGCCCCAAGGACCTGACCCCCTTCCTGACAACCCTGT   
               
               
                 
                   GGCTGCTCCTGGGCCATAGCAGAGTGCCTAGAGTGCGGGCCGAGGAATGCTGCGAGTTCATCAACGTGAACCACC 
                 
               
               
                 
                   CCCCCGAGCGGTGCTACGACTTCAAGATGTGCAACCGGTTCACCGTGGCCCTGAGATGCCCCGACGGCGAAGTGT 
                 
               
               
                 
                   GCTACAGCCCCGAGAAAACCGCCGAGATCCGGGGCATCGTGACCACCATGACCCACAGCCTGACCCGGCAGGTGG 
                 
               
               
                 
                   TGCACAACAAGCTGACCAGCTGCAACTACAACCCCCTGTACCTGGAAGCCGACGGCCGGATCAGATGCGGCAAAG 
                 
               
               
                 
                   TGAACGACAAGGCCCAGTACCTGCTGGGAGCCGCCGGAAGCGTGCCCTACCGGTGGATCAACCTGGAATACGACA 
                 
               
               
                 
                   AGATCACCCGGATCGTGGGCCTGGACCAGTACCTGGAAAGCGTGAAGAAGCACAAGCGGCTGGACGTGTGCAGAG 
                 
               
               
                   CCAAGATGGGCTACATGCTGCAG TGATAAGGCGCGCCAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTG 
               
               
                 TGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGT 
               
               
                 CTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGA 
               
               
                 AGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACC 
               
               
                 TGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCA 
               
               
                 CGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGC 
               
               
                 CCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTT 
               
               
                 AAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATAAT ATGCTGCGGCTGC   
               
               
                 
                   TGCTGAGACACCACTTCCACTGCCTGCTGCTGTGTGCCGTGTGGGCCACCCCTTGTCTGGCCAGCCCTTGGAGCA 
                 
               
               
                 
                   CCCTGACCGCCAACCAGAACCCTAGCCCCCCTTGGTCCAAGCTGACCTACAGCAAGCCCCACGACGCCGCCACCT 
                 
               
               
                 
                   TCTACTGCCCCTTTCTGTACCCCAGCCCTCCCAGAAGCCCCCTGCAGTTCAGCGGCTTCCAGAGAGTGTCCACCG 
                 
               
               
                 
                   GCCCTGAGTGCCGGAACGAGACACTGTACCTGCTGTACAACCGGGAGGGCCAGACACTGGTGGAGCGGAGCAGCA 
                 
               
               
                 
                   CCTGGGTGAAAAAAGTGATCTGGTATCTGAGCGGCCGGAACCAGACCATCCTGCAGCGGATGCCCAGAACCGCCA 
                 
               
               
                 
                   GCAAGCCCAGCGACGGCAACGTGCAGATCAGCGTGGAGGACGCCAAAATCTTCGGAGCCCACATGGTGCCCAAGC 
                 
               
               
                 
                   AGACCAAGCTGCTGAGATTCGTGGTCAACGACGGCACCAGATATCAGATGTGCGTGATGAAGCTGGAAAGCTGGG 
                 
               
               
                 
                   CCCACGTGTTCCGGGACTACTCCGTGAGCTTCCAGGTCCGGCTGACCTTCACCGAGGCCAACAACCAGACCTACA 
                 
               
               
                   CCTTCTGCACCCACCCCAACCTGATCGTG TGATAAGTACCTTTGTACGCCTGTTTTATACCCCCTCCCTGATTTG 
               
               
                 CAACTTAGAAGCAACGCAAACCAGATCAATAGTAGGTGTGACATACCAGTCGCATCTTGATCAAGCACTTCTGTA 
               
               
                 TCCCCGGACCGAGTATCAATAGACTGTGCACACGGTTGAAGGAGAAAACGTCCGTTACCCGGCTAACTACTTCGA 
               
               
                 GAAGCCTAGTAACGCCATTGAAGTTGCAGAGTGTTTCGCTCAGCACTCCCCCCGTGTAGATCAGGTCGATGAGTC 
               
               
                 ACCGCATTCCCCACGGGCGACCGTGGCGGTGGCTGCGTTGGCGGCCTGCCTATGGGGTAACCCATAGGACGCTCT 
               
               
                 AATACGGACATGGCGTGAAGAGTCTATTGAGCTAGTTAGTAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACT 
               
               
                 GCGGAGCACATACCCTTAATCCAAAGGGCAGTGTGTCGTAACGGGCAACTCTGCAGCGGAACCGACTACTTTGGG 
               
               
                 TGTCCGTGTTTCTTTTTATTCTTGTATTGGCTGCTTATGGTGACAATTAAAGAATTGTTACCATATAGCTATTGG 
               
               
                 ATTGGCCATCCAGTGTCAAACAGAGCTATTGTATATCTCTTTGTTGGATTCACACCTCTCACTCTTGAAACGTTA 
               
               
                 CACACCCTCAATTACATTATACTGCTGAACACGAAGCGC ATATGCGGCTGTGCAGAGTGTGGCTGTCCGTGTGCC   
               
               
                 
                   TGTGTGCCGTGGTGCTGGGCCAGTGCCAGAGAGAGACAGCCGAGAAGAACGACTACTACCGGGTGCCCCACTACT 
                 
               
               
                 
                   GGGATGCCTGCAGCAGAGCCCTGCCCGACCAGACCCGGTACAAATACGTGGAGCAGCTCGTGGACCTGACCCTGA 
                 
               
               
                 
                   ACTACCACTACGACGCCAGCCACGGCCTGGACAACTTCGACGTGCTGAAGCGGATCAACGTGACCGAGGTGTCCC 
                 
               
               
                 
                   TGCTGATCAGCGACTTCCGGCGGCAGAACAGAAGAGGCGGCACCAACAAGCGGACCACCTTCAACGCCGCTGGCT 
                 
               
               
                   CTCTGGCCCCTCACGCCAGATCCCTGGAATTCAGCGTGCGGCTGTTCGCCAAC TGATAACGTTGCATCCTGCAGG 
               
               
                 ATACAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCGCCTTAAAATTTTTATTTTAT 
               
               
                 TTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 
               
               
                 AAGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGACCTGGGCATCCGAAGGAGGACGCACGTCCACTCGGAT 
               
               
                 GGCTAAGGGAGAGCCACGTTTAAACGCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTAT 
               
               
                 TACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGAT 
               
               
                 TTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCG 
               
               
                 ACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTGGTCCACCTACAACAAAGCTCTCATCA 
               
               
                 ACCGTGGCTCCCTCACTTTCTGGCTGGATGATGGGGCGATTCAGGCCTGGTATGAGTCAGCAACACCTTCTTCAC 
               
               
                 GAGGCAGACCTCAGCGCTAGCGGAGTGTATACTGGCTTACTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCT 
               
               
                 TCATGTGGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCG 
               
               
                 CTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGAGCGGAAATGGCTTACGAACGGGGCGGAGATTTCCTG 
               
               
                 GAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGGCCGCGGCAAAGCCGTTTTTCCATAGGCTCCGCCCCC 
               
               
                 CTGACAAGCATCACGAAATCTGACGCTCAAATCAGTGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT 
               
               
                 TTCCCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGGTGTCATTCCGCTGTTATGGCC 
               
               
                 GCGTTTGTCTCATTCCACGCCTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTGGACTGTATGCACGAAC 
               
               
                 CCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGAAAGACATGCAAAAG 
               
               
                 CACCACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAGTTAGTCTTGAAGTCATGCGCCGGTTAAGGCTAAACT 
               
               
                 GAAAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAGTTACCTCGGTTCAAAGAGTTGGTAGCTCAGAGAACC 
               
               
                 TTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCAGAGCAAGAGATTACGCGCAGACCAAAACGATCTCAA 
               
               
                 GAAGATCATCTTATTAAGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTA 
               
               
                 TCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAA 
               
               
                 ACTTGGTCTGACAGTTATTAGAAAAATTCATCCAGCAGACGATAAAACGCAATACGCTGGCTATCCGGTGCCGCA 
               
               
                 ATGCCATACAGCACCAGAAAACGATCCGCCCATTCGCCGCCCAGTTCTTCCGCAATATCACGGGTGGCCAGCGCA 
               
               
                 ATATCCTGATAACGATCCGCCACGCCCAGACGGCCGCAATCAATAAAGCCGCTAAAACGGCCATTTTCCACCATA 
               
               
                 ATGTTCGGCAGGCACGCATCACCATGGGTCACCACCAGATCTTCGCCATCCGGCATGCTCGCTTTCAGACGCGCA 
               
               
                 AACAGCTCTGCCGGTGCCAGGCCCTGATGTTCTTCATCCAGATCATCCTGATCCACCAGGCCCGCTTCCATACGG 
               
               
                 GTACGCGCACGTTCAATACGATGTTTCGCCTGATGATCAAACGGACAGGTCGCCGGGTCCAGGGTATGCAGACGA 
               
               
                 CGCATGGCATCCGCCATAATGCTCACTTTTTCTGCCGGCGCCAGATGGCTAGACAGCAGATCCTGACCCGGCACT 
               
               
                 TCGCCCAGCAGCAGCCAATCACGGCCCGCTTCGGTCACCACATCCAGCACCGCCGCACACGGAACACCGGTGGTG 
               
               
                 GCCAGCCAGCTCAGACGCGCCGCTTCATCCTGCAGCTCGTTCAGCGCACCGCTCAGATCGGTTTTCACAAACAGC 
               
               
                 ACCGGACGACCCTGCGCGCTCAGACGAAACACCGCCGCATCAGAGCAGCCAATGGTCTGCTGCGCCCAATCATAG 
               
               
                 CCAAACAGACGTTCCACCCACGCTGCCGGGCTACCCGCATGCAGGCCATCCTGTTCAATCATACTCTTCCTTTTT 
               
               
                 CAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAA 
               
               
                 CAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCG 
               
               
                 CGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAG 
               
               
                 AATAGACCGAGATAGGGTTGAGTGGCCGCTACAGGGCGCTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAA 
               
               
                 GGGCGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTG 
               
               
                 GGTAACGCCAGGGTTTTCCCAGTCACACGCGTAATACGACTCACTATAG 
               
               
                   
               
               
                 A556 Vector: SGP-gHso16His-SGP-gL-SGP-UL128-SGP-UL130-SGP-UL131 (SEQ ID 
               
               
                 NO: 67): 
               
               
                 ATAGGCGGCGCATGAGAGAAGCCCAGACCAATTACCTACCCAAAATGGAGAAAGTTCACGTTGACATCGAGGAAG 
               
               
                 ACAGCCCATTCCTCAGAGCTTTGCAGCGGAGCTTCCCGCAGTTTGAGGTAGAAGCCAAGCAGGTCACTGATAATG 
               
               
                 ACCATGCTAATGCCAGAGCGTTTTCGCATCTGGCTTCAAAACTGATCGAAACGGAGGTGGACCCATCCGACACGA 
               
               
                 TCCTTGACATTGGAAGTGCGCCCGCCCGCAGAATGTATTCTAAGCACAAGTATCATTGTATCTGTCCGATGAGAT 
               
               
                 GTGCGGAAGATCCGGACAGATTGTATAAGTATGCAACTAAGCTGAAGAAAAACTGTAAGGAAATAACTGATAAGG 
               
               
                 AATTGGACAAGAAAATGAAGGAGCTCGCCGCCGTCATGAGCGACCCTGACCTGGAAACTGAGACTATGTGCCTCC 
               
               
                 ACGACGACGAGTCGTGTCGCTACGAAGGGCAAGTCGCTGTTTACCAGGATGTATACGCGGTTGACGGACCGACAA 
               
               
                 GTCTCTATCACCAAGCCAATAAGGGAGTTAGAGTCGCCTACTGGATAGGCTTTGACACCACCCCTTTTATGTTTA 
               
               
                 AGAACTTGGCTGGAGCATATCCATCATACTCTACCAACTGGGCCGACGAAACCGTGTTAACGGCTCGTAACATAG 
               
               
                 GCCTATGCAGCTCTGACGTTATGGAGCGGTCACGTAGAGGGATGTCCATTCTTAGAAAGAAGTATTTGAAACCAT 
               
               
                 CCAACAATGTTCTATTCTCTGTTGGCTCGACCATCTACCACGAGAAGAGGGACTTACTGAGGAGCTGGCACCTGC 
               
               
                 CGTCTGTATTTCACTTACGTGGCAAGCAAAATTACACATGTCGGTGTGAGACTATAGTTAGTTGCGACGGGTACG 
               
               
                 TCGTTAAAAGAATAGCTATCAGTCCAGGCCTGTATGGGAAGCCTTCAGGCTATGCTGCTACGATGCACCGCGAGG 
               
               
                 GATTCTTGTGCTGCAAAGTGACAGACACATTGAACGGGGAGAGGGTCTCTTTTCCCGTGTGCACGTATGTGCCAG 
               
               
                 CTACATTGTGTGACCAAATGACTGGCATACTGGCAACAGATGTCAGTGCGGACGACGCGCAAAAACTGCTGGTTG 
               
               
                 GGCTCAACCAGCGTATAGTCGTCAACGGTCGCACCCAGAGAAACACCAATACCATGAAAAATTACCTTTTGCCCG 
               
               
                 TAGTGGCCCAGGCATTTGCTAGGTGGGCAAAGGAATATAAGGAAGATCAAGAAGATGAAAGGCCACTAGGACTAC 
               
               
                 GAGATAGACAGTTAGTCATGGGGTGTTGTTGGGCTTTTAGAAGGCACAAGATAACATCTATTTATAAGCGCCCGG 
               
               
                 ATACCCAAACCATCATCAAAGTGAACAGCGATTTCCACTCATTCGTGCTGCCCAGGATAGGCAGTAACACATTGG 
               
               
                 AGATCGGGCTGAGAACAAGAATCAGGAAAATGTTAGAGGAGCACAAGGAGCCGTCACCTCTCATTACCGCCGAGG 
               
               
                 ACGTACAAGAAGCTAAGTGCGCAGCCGATGAGGCTAAGGAGGTGCGTGAAGCCGAGGAGTTGCGCGCAGCTCTAC 
               
               
                 CACCTTTGGCAGCTGATGTTGAGGAGCCCACTCTGGAAGCCGATGTAGACTTGATGTTACAAGAGGCTGGGGCCG 
               
               
                 GCTCAGTGGAGACACCTCGTGGCTTGATAAAGGTTACCAGCTACGATGGCGAGGACAAGATCGGCTCTTACGCTG 
               
               
                 TGCTTTCTCCGCAGGCTGTACTCAAGAGTGAAAAATTATCTTGCATCCACCCTCTCGCTGAACAAGTCATAGTGA 
               
               
                 TAACACACTCTGGCCGAAAAGGGCGTTATGCCGTGGAACCATACCATGGTAAAGTAGTGGTGCCAGAGGGACATG 
               
               
                 CAATACCCGTCCAGGACTTTCAAGCTCTGAGTGAAAGTGCCACCATTGTGTACAACGAACGTGAGTTCGTAAACA 
               
               
                 GGTACCTGCACCATATTGCCACACATGGAGGAGCGCTGAACACTGATGAAGAATATTACAAAACTGTCAAGCCCA 
               
               
                 GCGAGCACGACGGCGAATACCTGTACGACATCGACAGGAAACAGTGCGTCAAGAAAGAACTAGTCACTGGGCTAG 
               
               
                 GGCTCACAGGCGAGCTGGTGGATCCTCCCTTCCATGAATTCGCCTACGAGAGTCTGAGAACACGACCAGCCGCTC 
               
               
                 CTTACCAAGTACCAACCATAGGGGTGTATGGCGTGCCAGGATCAGGCAAGTCTGGCATCATTAAAAGCGCAGTCA 
               
               
                 CCAAAAAAGATCTAGTGGTGAGCGCCAAGAAAGAAAACTGTGCAGAAATTATAAGGGACGTCAAGAAAATGAAAG 
               
               
                 GGCTGGACGTCAATGCCAGAACTGTGGACTCAGTGCTCTTGAATGGATGCAAACACCCCGTAGAGACCCTGTATA 
               
               
                 TTGACGAAGCTTTTGCTTGTCATGCAGGTACTCTCAGAGCGCTCATAGCCATTATAAGACCTAAAAAGGCAGTGC 
               
               
                 TCTGCGGGGATCCCAAACAGTGCGGTTTTTTTAACATGATGTGCCTGAAAGTGCATTTTAACCACGAGATTTGCA 
               
               
                 CACAAGTCTTCCACAAAAGCATCTCTCGCCGTTGCACTAAATCTGTGACTTCGGTCGTCTCAACCTTGTTTTACG 
               
               
                 ACAAAAAAATGAGAACGACGAATCCGAAAGAGACTAAGATTGTGATTGACACTACCGGCAGTACCAAACCTAAGC 
               
               
                 AGGACGATCTCATTCTCACTTGTTTCAGAGGGTGGGTGAAGCAGTTGCAAATAGATTACAAAGGCAACGAAATAA 
               
               
                 TGACGGCAGCTGCCTCTCAAGGGCTGACCCGTAAAGGTGTGTATGCCGTTCGGTACAAGGTGAATGAAAATCCTC 
               
               
                 TGTACGCACCCACCTCAGAACATGTGAACGTCCTACTGACCCGCACGGAGGACCGCATCGTGTGGAAAACACTAG 
               
               
                 CCGGCGACCCATGGATAAAAACACTGACTGCCAAGTACCCTGGGAATTTCACTGCCACGATAGAGGAGTGGCAAG 
               
               
                 CAGAGCATGATGCCATCATGAGGCACATCTTGGAGAGACCGGACCCTACCGACGTCTTCCAGAATAAGGCAAACG 
               
               
                 TGTGTTGGGCCAAGGCTTTAGTGCCGGTGCTGAAGACCGCTGGCATAGACATGACCACTGAACAATGGAACACTG 
               
               
                 TGGATTATTTTGAAACGGACAAAGCTCACTCAGCAGAGATAGTATTGAACCAACTATGCGTGAGGTTCTTTGGAC 
               
               
                 TCGATCTGGACTCCGGTCTATTTTCTGCACCCACTGTTCCGTTATCCATTAGGAATAATCACTGGGATAACTCCC 
               
               
                 CGTCGCCTAACATGTACGGGCTGAATAAAGAAGTGGTCCGTCAGCTCTCTCGCAGGTACCCACAACTGCCTCGGG 
               
               
                 CAGTTGCCACTGGAAGAGTCTATGACATGAACACTGGTACACTGCGCAATTATGATCCGCGCATAAACCTAGTAC 
               
               
                 CTGTAAACAGAAGACTGCCTCATGCTTTAGTCCTCCACCATAATGAACACCCACAGAGTGACTTTTCTTCATTCG 
               
               
                 TCAGCAAATTGAAGGGCAGAACTGTCCTGGTGGTCGGGGAAAAGTTGTCCGTCCCAGGCAAAATGGTTGACTGGT 
               
               
                 TGTCAGACCGGCCTGAGGCTACCTTCAGAGCTCGGCTGGATTTAGGCATCCCAGGTGATGTGCCCAAATATGACA 
               
               
                 TAATATTTGTTAATGTGAGGACCCCATATAAATACCATCACTATCAGCAGTGTGAAGACCATGCCATTAAGCTTA 
               
               
                 GCATGTTGACCAAGAAAGCTTGTCTGCATCTGAATCCCGGCGGAACCTGTGTCAGCATAGGTTATGGTTACGCTG 
               
               
                 ACAGGGCCAGCGAAAGCATCATTGGTGCTATAGCGCGGCAGTTCAAGTTTTCCCGGGTATGCAAACCGAAATCCT 
               
               
                 CACTTGAAGAGACGGAAGTTCTGTTTGTATTCATTGGGTACGATCGCAAGGCCCGTACGCACAATCCTTACAAGC 
               
               
                 TTTCATCAACCTTGACCAACATTTATACAGGTTCCAGACTCCACGAAGCCGGATGTGCACCCTCATATCATGTGG 
               
               
                 TGCGAGGGGATATTGCCACGGCCACCGAAGGAGTGATTATAAATGCTGCTAACAGCAAAGGACAACCTGGCGGAG 
               
               
                 GGGTGTGCGGAGCGCTGTATAAGAAATTCCCGGAAAGCTTCGATTTACAGCCGATCGAAGTAGGAAAAGCGCGAC 
               
               
                 TGGTCAAAGGTGCAGCTAAACATATCATTCATGCCGTAGGACCAAACTTCAACAAAGTTTCGGAGGTTGAAGGTG 
               
               
                 ACAAACAGTTGGCAGAGGCTTATGAGTCCATCGCTAAGATTGTCAACGATAACAATTACAAGTCAGTAGCGATTC 
               
               
                 CACTGTTGTCCACCGGCATCTTTTCCGGGAACAAAGATCGACTAACCCAATCATTGAACCATTTGCTGACAGCTT 
               
               
                 TAGACACCACTGATGCAGATGTAGCCATATACTGCAGGGACAAGAAATGGGAAATGACTCTCAAGGAAGCAGTGG 
               
               
                 CTAGGAGAGAAGCAGTGGAGGAGATATGCATATCCGACGACTCTTCAGTGACAGAACCTGATGCAGAGCTGGTGA 
               
               
                 GGGTGCATCCGAAGAGTTCTTTGGCTGGAAGGAAGGGCTACAGCACAAGCGATGGCAAAACTTTCTCATATTTGG 
               
               
                 AAGGGACCAAGTTTCACCAGGCGGCCAAGGATATAGCAGAAATTAATGCCATGTGGCCCGTTGCAACGGAGGCCA 
               
               
                 ATGAGCAGGTATGCATGTATATCCTCGGAGAAAGCATGAGCAGTATTAGGTCGAAATGCCCCGTCGAAGAGTCGG 
               
               
                 AAGCCTCCACACCACCTAGCACGCTGCCTTGCTTGTGCATCCATGCCATGACTCCAGAAAGAGTACAGCGCCTAA 
               
               
                 AAGCCTCACGTCCAGAACAAATTACTGTGTGCTCATCCTTTCCATTGCCGAAGTATAGAATCACTGGTGTGCAGA 
               
               
                 AGATCCAATGCTCCCAGCCTATATTGTTCTCACCGAAAGTGCCTGCGTATATTCATCCAAGGAAGTATCTCGTGG 
               
               
                 AAACACCACCGGTAGACGAGACTCCGGAGCCATCGGCAGAGAACCAATCCACAGAGGGGACACCTGAACAACCAC 
               
               
                 CACTTATAACCGAGGATGAGACCAGGACTAGAACGCCTGAGCCGATCATCATCGAAGAGGAAGAAGAGGATAGCA 
               
               
                 TAAGTTTGCTGTCAGATGGCCCGACCCACCAGGTGCTGCAAGTCGAGGCAGACATTCACGGGCCGCCCTCTGTAT 
               
               
                 CTAGCTCATCCTGGTCCATTCCTCATGCATCCGACTTTGATGTGGACAGTTTATCCATACTTGACACCCTGGAGG 
               
               
                 GAGCTAGCGTGACCAGCGGGGCAACGTCAGCCGAGACTAACTCTTACTTCGCAAAGAGTATGGAGTTTCTGGCGC 
               
               
                 GACCGGTGCCTGCGCCTCGAACAGTATTCAGGAACCCTCCACATCCCGCTCCGCGCACAAGAACACCGTCACTTG 
               
               
                 CACCCAGCAGGGCCTGCTCGAGAACCAGCCTAGTTTCCACCCCGCCAGGCGTGAATAGGGTGATCACTAGAGAGG 
               
               
                 AGCTCGAGGCGCTTACCCCGTCACGCACTCCTAGCAGGTCGGTCTCGAGAACCAGCCTGGTCTCCAACCCGCCAG 
               
               
                 GCGTAAATAGGGTGATTACAAGAGAGGAGTTTGAGGCGTTCGTAGCACAACAACAATGACGGTTTGATGCGGGTG 
               
               
                 CATACATCTTTTCCTCCGACACCGGTCAAGGGCATTTACAACAAAAATCAGTAAGGCAAACGGTGCTATCCGAAG 
               
               
                 TGGTGTTGGAGAGGACCGAATTGGAGATTTCGTATGCCCCGCGCCTCGACCAAGAAAAAGAAGAATTACTACGCA 
               
               
                 AGAAATTACAGTTAAATCCCACACCTGCTAACAGAAGCAGATACCAGTCCAGGAAGGTGGAGAACATGAAAGCCA 
               
               
                 TAACAGCTAGACGTATTCTGCAAGGCCTAGGGCATTATTTGAAGGCAGAAGGAAAAGTGGAGTGCTACCGAACCC 
               
               
                 TGCATCCTGTTCCTTTGTATTCATCTAGTGTGAACCGTGCCTTTTCAAGCCCCAAGGTCGCAGTGGAAGCCTGTA 
               
               
                 ACGCCATGTTGAAAGAGAACTTTCCGACTGTGGCTTCTTACTGTATTATTCCAGAGTACGATGCCTATTTGGACA 
               
               
                 TGGTTGACGGAGCTTCATGCTGCTTAGACACTGCCAGTTTTTGCCCTGCAAAGCTGCGCAGCTTTCCAAAGAAAC 
               
               
                 ACTCCTATTTGGAACCCACAATACGATCGGCAGTGCCTTCAGCGATCCAGAACACGCTCCAGAACGTCCTGGCAG 
               
               
                 CTGCCACAAAAAGAAATTGCAATGTCACGCAAATGAGAGAATTGCCCGTATTGGATTCGGCGGCCTTTAATGTGG 
               
               
                 AATGCTTCAAGAAATATGCGTGTAATAATGAATATTGGGAAACGTTTAAAGAAAACCCCATCAGGCTTACTGAAG 
               
               
                 AAAACGTGGTAAATTACATTACCAAATTAAAAGGACCAAAAGCTGCTGCTCTTTTTGCGAAGACACATAATTTGA 
               
               
                 ATATGTTGCAGGACATACCAATGGACAGGTTTGTAATGGACTTAAAGAGAGACGTGAAAGTGACTCCAGGAACAA 
               
               
                 AACATACTGAAGAACGGCCCAAGGTACAGGTGATCCAGGCTGCCGATCCGCTAGCAACAGCGTATCTGTGCGGAA 
               
               
                 TCCACCGAGAGCTGGTTAGGAGATTAAATGCGGTCCTGCTTCCGAACATTCATACACTGTTTGATATGTCGGCTG 
               
               
                 AAGACTTTGACGCTATTATAGCCGAGCACTTCCAGCCTGGGGATTGTGTTCTGGAAACTGACATCGCGTCGTTTG 
               
               
                 ATAAAAGTGAGGACGACGCCATGGCTCTGACCGCGTTAATGATTCTGGAAGACTTAGGTGTGGACGCAGAGCTGT 
               
               
                 TGACGCTGATTGAGGCGGCTTTCGGCGAAATTTCATCAATACATTTGCCCACTAAAACTAAATTTAAATTCGGAG 
               
               
                 CCATGATGAAATCTGGAATGTTCCTCACACTGTTTGTGAACACAGTCATTAACATTGTAATCGCAAGCAGAGTGT 
               
               
                 TGAGAGAACGGCTAACCGGATCACCATGTGCAGCATTCATTGGAGATGACAATATCGTGAAAGGAGTCAAATCGG 
               
               
                 ACAAATTAATGGCAGACAGGTGCGCCACCTGGTTGAATATGGAAGTCAAGATTATAGATGCTGTGGTGGGCGAGA 
               
               
                 AAGCGCCTTATTTCTGTGGAGGGTTTATTTTGTGTGACTCCGTGACCGGCACAGCGTGCCGTGTGGCAGACCCCC 
               
               
                 TAAAAAGGCTGTTTAAGCTTGGCAAACCTCTGGCAGCAGACGATGAACATGATGATGACAGGAGAAGGGCATTGC 
               
               
                 ATGAAGAGTCAACACGCTGGAACCGAGTGGGTATTCTTTCAGAGCTGTGCAAGGCAGTAGAATCAAGGTATGAAA 
               
               
                 CCGTAGGAACTTCCATCATAGTTATGGCCATGACTACTCTAGCTAGCAGTGTTAAATCATTCAGCTACCTGAGAG 
               
               
                 GGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGAGGCCTGGCCT   
               
               
                 
                   GCCCTCCTACCTGATCATCCTGGCCGTGTGCCTGTTCAGCCACCTGCTGTCCAGCAGATACGGCGCCGAGGCCGT 
                 
               
               
                 
                   GAGCGAGCCCCTGGACAAGGCTTTCCACCTGCTGCTGAACACCTACGGCAGACCCATCCGGTTTCTGCGGGAGAA 
                 
               
               
                 
                   CACCACCCAGTGCACCTACAACAGCAGCCTGCGGAACAGCACCGTCGTGAGAGAGAACGCCATCAGCTTCAACTT 
                 
               
               
                 
                   TTTCCAGAGCTACAACCAGTACTACGTGTTCCACATGCCCAGATGCCTGTTTGCCGGCCCTCTGGCCGAGCAGTT 
                 
               
               
                 
                   CCTGAACCAGGTGGACCTGACCGAGACACTGGAAAGATACCAGCAGCGGCTGAATACCTACGCCCTGGTGTCCAA 
                 
               
               
                 
                   GGACCTGGCCAGCTACCGGTCCTTTAGCCAGCAGCTCAAGGCTCAGGATAGCCTCGGCGAGCAGCCTACCACCGT 
                 
               
               
                 
                   GCCCCCTCCCATCGACCTGAGCATCCCCCACGTGTGGATGCCTCCCCAGACCACCCCTCACGGCTGGACCGAGAG 
                 
               
               
                 
                   CCACACCACCTCCGGCCTGCACAGACCCCACTTCAACCAGACCTGCATCCTGTTCGACGGCCACGACCTGCTGTT 
                 
               
               
                 
                   TAGCACCGTGACCCCCTGCCTGCACCAGGGCTTCTACCTGATCGACGAGCTGAGATACGTGAAGATCACCCTGAC 
                 
               
               
                 
                   CGAGGATTTCTTCGTGGTCACCGTGTCCATCGACGACGACACCCCCATGCTGCTGATCTTCGGCCACCTGCCCAG 
                 
               
               
                 
                   AGTGCTGTTCAAGGCCCCCTACCAGCGGGACAACTTCATCCTGCGGCAGACCGAGAAGCACGAGCTGCTGGTGCT 
                 
               
               
                 
                   GGTCAAGAAGGACCAGCTGAACCGGCACTCCTACCTGAAGGACCCCGACTTCCTGGACGCCGCCCTGGACTTCAA 
                 
               
               
                 
                   CTACCTGGACCTGAGCGCCCTGCTGAGAAACAGCTTCCACAGATACGCCGTGGACGTGCTGAAGTCCGGACGGTG 
                 
               
               
                 
                   CCAGATGCTCGATCGGCGGACCGTGGAGATGGCCTTCGCCTATGCCCTCGCCCTGTTCGCCGCTGCCAGACAGGA 
                 
               
               
                 
                   AGAGGCTGGCGCCCAGGTGTCAGTGCCCAGAGCCCTGGATAGACAGGCCGCCCTGCTGCAGATCCAGGAATTCAT 
                 
               
               
                 
                   GATCACCTGCCTGAGCCAGACCCCCCCTAGAACCACCCTGCTGCTGTACCCCACAGCCGTGGATCTGGCCAAGAG 
                 
               
               
                 
                   GGCCCTGTGGACCCCCAACCAGATCACCGACATCACAAGCCTCGTGCGGCTCGTGTACATCCTGAGCAAGCAGAA 
                 
               
               
                 
                   CCAGCAGCACCTGATCCCCCAGTGGGCCCTGAGACAGATCGCCGACTTCGCCCTGAAGCTGCACAAGACCCATCT 
                 
               
               
                 
                   GGCCAGCTTTCTGAGCGCCTTCGCCAGGCAGGAACTGTACCTGATGGGCAGCCTGGTCCACAGCATGCTGGTGCA 
                 
               
               
                 
                   TACCACCGAGCGGCGGGAGATCTTCATCGTGGAGACAGGCCTGTGTAGCCTGGCCGAGCTGTCCCACTTTACCCA 
                 
               
               
                 
                   GCTGCTGGCCCACCCTCACCACGAGTACCTGAGCGACCTGTACACCCCCTGCAGCAGCAGCGGCAGACGGGACCA 
                 
               
               
                 
                   CAGCCTGGAACGGCTGACCAGACTGTTCCCCGATGCCACCGTGCCTGCTACAGTGCCTGCCGCCCTGTCCATCCT 
                 
               
               
                 
                   GTCCACCATGCAGCCCAGCACCCTGGAAACCTTCCCCGACCTGTTCTGCCTGCCCCTGGGCGAGAGCTTTAGCGC 
                 
               
               
                 
                   CCTGACCGTGTCCGAGCACGTGTCCTACATCGTGACCAATCAGTACCTGATCAAGGGCATCAGCTACCCCGTGTC 
                 
               
               
                 
                   CACCACAGTCGTGGGCCAGAGCCTGATCATCACCCAGACCGACAGCCAGACCAAGTGCGAGCTGACCCGGAACAT 
                 
               
               
                 
                   GCACACCACACACAGCATCACCGTGGCCCTGAACATCAGCCTGGAAAACTGCGCTTTCTGTCAGTCTGCCCTGCT 
                 
               
               
                 
                   GGAATACGACGATACCCAGGGCGTGATCAACATCATGTACATGCACGACAGCGACGACGTGCTGTTCGCCCTGGA 
                 
               
               
                 
                   CCCCTACAACGAGGTGGTGGTGTCCAGCCCCCGGACCCACTACCTGATGCTGCTGAAGAACGGCACCGTGCTGGA 
                 
               
               
                   AGTGACCGACGTGGTGGTGGACGCCACCGACGGCAGCGGATCTGGGTCCCACCATCACCATCACCAT TGATAATC 
               
               
                 TAGAGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGTGCAGAAG   
               
               
                 
                   GCCCGACTGCGGCTTCAGCTTCAGCCCTGGACCCGTGATCCTGCTGTGGTGCTGCCTGCTGCTGCCTATCGTGTC 
                 
               
               
                 CTCTGCCGCCGTGTCTGTGGCCCCTACAGCCGCCGAGAAGGTGCCAGCCGAGTGCCCCGAGCTGACCAGAAGATG 
               
               
                 
                   CCTGCTGGGCGAGGTGTTCGAGGGCGACAAGTACGAGAGCTGGCTGCGGCCCCTGGTCAACGTGACCGGCAGAGA 
                 
               
               
                 
                   TGGCCCCCTGAGCCAGCTGATCCGGTACAGACCCGTGACCCCCGAGGCCGCCAATAGCGTGCTGCTGGACGAGGC 
                 
               
               
                 
                   CTTCCTGGATACCCTGGCCCTGCTGTACAACAACCCCGACCAGCTGAGAGCCCTGCTGACCCTGCTGTCCAGCGA 
                 
               
               
                 
                   CACCGCCCCCAGATGGATGACCGTGATGCGGGGCTACAGCGAGTGTGGAGATGGCAGCCCTGCCGTGTACACCTG 
                 
               
               
                 
                   CGTGGACGACCTGTGCAGAGGCTACGACCTGACCAGACTGAGCTACGGCCGGTCCATCTTCACAGAGCACGTGCT 
                 
               
               
                 
                   GGGCTTCGAGCTGGTGCCCCCCAGCCTGTTCAACGTGGTGGTGGCCATCCGGAACGAGGCCACCAGAACCAACAG 
                 
               
               
                 
                   AGCCGTGCGGCTGCCTGTGTCTACAGCCGCTGCACCTGAGGGCATCACACTGTTCTACGGCCTGTACAACGCCGT 
                 
               
               
                 
                   GAAAGAGTTCTGCCTCCGGCACCAGCTGGATCCCCCCCTGCTGAGACACCTGGACAAGTACTACGCCGGCCTGCC 
                 
               
               
                   CCCAGAGCTGAAGCAGACCAGAGTGAACCTGCCCGCCCACAGCAGATATGGCCCTCAGGCCGTGGACGCCAGA TG 
               
               
                 ATAACGCCGGCGGCCCCTATAACTCTCTACGGCTAACCTGAATGGACTACGACATAGTCTAGTCCGCCAAG ATGA   
               
               
                 
                   GCCCCAAGGACCTGACCCCCTTCCTGACAACCCTGTGGCTGCTCCTGGGCCATAGCAGAGTGCCTAGAGTGCGGG 
                 
               
               
                 
                   CCGAGGAATGCTGCGAGTTCATCAACGTGAACCACCCCCCCGAGCGGTGCTACGACTTCAAGATGTGCAACCGGT 
                 
               
               
                 
                   TCACCGTGGCCCTGAGATGCCCCGACGGCGAAGTGTGCTACAGCCCCGAGAAAACCGCCGAGATCCGGGGCATCG 
                 
               
               
                 
                   TGACCACCATGACCCACAGCCTGACCCGGCAGGTGGTGCACAACAAGCTGACCAGCTGCAACTACAACCCCCTGT 
                 
               
               
                 
                   ACCTGGAAGCCGACGGCCGGATCAGATGCGGCAAAGTGAACGACAAGGCCCAGTACCTGCTGGGAGCCGCCGGAA 
                 
               
               
                 
                   GCGTGCCCTACCGGTGGATCAACCTGGAATACGACAAGATCACCCGGATCGTGGGCCTGGACCAGTACCTGGAAA 
                 
               
               
                 
                   GCGTGAAGAAGCACAAGCGGCTGGACGTGTGCAGAGCCAAGATGGGCTACATGCTGCAGTGATAAGGCGCGCCAA 
                 
               
               
                 CGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTC 
               
               
                 TTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGC 
               
               
                 CAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTC 
               
               
                 TGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATA 
               
               
                 AGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCT 
               
               
                 CTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCC 
               
               
                 TCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGT 
               
               
                 TTTCCTTTGAAAAACACGATAAT ATGCTGCGGCTGCTGCTGAGACACCACTTCCACTGCCTGCTGCTGTGTGCCG   
               
               
                 
                   TGTGGGCCACCCCTTGTCTGGCCAGCCCTTGGAGCACCCTGACCGCCAACCAGAACCCTAGCCCCCCTTGGTCCA 
                 
               
               
                 
                   AGCTGACCTACAGCAAGCCCCACGACGCCGCCACCTTCTACTGCCCCTTTCTGTACCCCAGCCCTCCCAGAAGCC 
                 
               
               
                 
                   CCCTGCAGTTCAGCGGCTTCCAGAGAGTGTCCACCGGCCCTGAGTGCCGGAACGAGACACTGTACCTGCTGTACA 
                 
               
               
                 
                   ACCGGGAGGGCCAGACACTGGTGGAGCGGAGCAGCACCTGGGTGAAAAAAGTGATCTGGTATCTGAGCGGCCGGA 
                 
               
               
                 
                   ACCAGACCATCCTGCAGCGGATGCCCAGAACCGCCAGCAAGCCCAGCGACGGCAACGTGCAGATCAGCGTGGAGG 
                 
               
               
                 
                   ACGCCAAAATCTTCGGAGCCCACATGGTGCCCAAGCAGACCAAGCTGCTGAGATTCGTGGTCAACGACGGCACCA 
                 
               
               
                 
                   GATATCAGATGTGCGTGATGAAGCTGGAAAGCTGGGCCCACGTGTTCCGGGACTACTCCGTGAGCTTCCAGGTCC 
                 
               
               
                   GGCTGACCTTCACCGAGGCCAACAACCAGACCTACACCTTCTGCACCCACCCCAACCTGATCGTGTG ATAAGTAC 
               
               
                 CTTTGTACGCCTGTTTTATACCCCCTCCCTGATTTGCAACTTAGAAGCAACGCAAACCAGATCAATAGTAGGTGT 
               
               
                 GACATACCAGTCGCATCTTGATCAAGCACTTCTGTATCCCCGGACCGAGTATCAATAGACTGTGCACACGGTTGA 
               
               
                 AGGAGAAAACGTCCGTTACCCGGCTAACTACTTCGAGAAGCCTAGTAACGCCATTGAAGTTGCAGAGTGTTTCGC 
               
               
                 TCAGCACTCCCCCCGTGTAGATCAGGTCGATGAGTCACCGCATTCCCCACGGGCGACCGTGGCGGTGGCTGCGTT 
               
               
                 GGCGGCCTGCCTATGGGGTAACCCATAGGACGCTCTAATACGGACATGGCGTGAAGAGTCTATTGAGCTAGTTAG 
               
               
                 TAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTGCGGAGCACATACCCTTAATCCAAAGGGCAGTGTGTCGT 
               
               
                 AACGGGCAACTCTGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCTTTTTATTCTTGTATTGGCTGCTTATG 
               
               
                 GTGACAATTAAAGAATTGTTACCATATAGCTATTGGATTGGCCATCCAGTGTCAAACAGAGCTATTGTATATCTC 
               
               
                 TTTGTTGGATTCACACCTCTCACTCTTGAAACGTTACACACCCTCAATTACATTATACTGCTGAACACGAAGCGC 
               
               
                 AT ATGCGGCTGTGCAGAGTGTGGCTGTCCGTGTGCCTGTGTGCCGTGGTGCTGGGCCAGTGCCAGAGAGAGACAG   
               
               
                 
                   CCGAGAAGAACGACTACTACCGGGTGCCCCACTACTGGGATGCCTGCAGCAGAGCCCTGCCCGACCAGACCCGGT 
                 
               
               
                 
                   ACAAATACGTGGAGCAGCTCGTGGACCTGACCCTGAACTACCACTACGACGCCAGCCACGGCCTGGACAACTTCG 
                 
               
               
                 
                   ACGTGCTGAAGCGGATCAACGTGACCGAGGTGTCCCTGCTGATCAGCGACTTCCGGCGGCAGAACAGAAGAGGCG 
                 
               
               
                 
                   GCACCAACAAGCGGACCACCTTCAACGCCGCTGGCTCTCTGGCCCCTCACGCCAGATCCCTGGAATTCAGCGTGC 
                 
               
               
                   GGCTGTTCGCCAAC TGATAACGTTGCATCCTGCAGGATACAGCAGCAATTGGCAAGCTGCTTACATAGAACTCGC 
               
               
                 GGCGATTGGCATGCCGCCTTAAAATTTTTATTTTATTTTTCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATAT 
               
               
                 TTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGTCGGCATGGCATCTCCACCTCCTCGCGGTCCGAC 
               
               
                 CTGGGCATCCGAAGGAGGACGCACGTCCACTCGGATGGCTAAGGGAGAGCCACGTTTAAACGCTAGAGCAAGACG 
               
               
                 TTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATG 
               
               
                 ATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTT 
               
               
                 TTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAA 
               
               
                 TCACCAACTGGTCCACCTACAACAAAGCTCTCATCAACCGTGGCTCCCTCACTTTCTGGCTGGATGATGGGGCGA 
               
               
                 TTCAGGCCTGGTATGAGTCAGCAACACCTTCTTCACGAGGCAGACCTCAGCGCTAGCGGAGTGTATACTGGCTTA 
               
               
                 CTATGTTGGCACTGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCTGCACCGGTGCGTCAG 
               
               
                 CAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCACTGACTCGCTACGCTCGGTCGTTCGACTGCGGCGA 
               
               
                 GCGGAAATGGCTTACGAACGGGGCGGAGATTTCCTGGAAGATGCCAGGAAGATACTTAACAGGGAAGTGAGAGGG 
               
               
                 CCGCGGCAAAGCCGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGTGGT 
               
               
                 GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCTGGCGGCTCCCTCGTGCGCTCTCCTGTTCCTGC 
               
               
                 CTTTCGGTTTACCGGTGTCATTCCGCTGTTATGGCCGCGTTTGTCTCATTCCACGCCTGACACTCAGTTCCGGGT 
               
               
                 AGGCAGTTCGCTCCAAGCTGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACT 
               
               
                 ATCGTCTTGAGTCCAACCCGGAAAGACATGCAAAAGCACCACTGGCAGCAGCCACTGGTAATTGATTTAGAGGAG 
               
               
                 TTAGTCTTGAAGTCATGCGCCGGTTAAGGCTAAACTGAAAGGACAAGTTTTGGTGACTGCGCTCCTCCAAGCCAG 
               
               
                 TTACCTCGGTTCAAAGAGTTGGTAGCTCAGAGAACCTTCGAAAAACCGCCCTGCAAGGCGGTTTTTTCGTTTTCA 
               
               
                 GAGCAAGAGATTACGCGCAGACCAAAACGATCTCAAGAAGATCATCTTATTAAGGGGTCTGACGCTCAGTGGAAC 
               
               
                 GAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAA 
               
               
                 TGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTATTAGAAAAATTCATCCAGCAGA 
               
               
                 CGATAAAACGCAATACGCTGGCTATCCGGTGCCGCAATGCCATACAGCACCAGAAAACGATCCGCCCATTCGCCG 
               
               
                 CCCAGTTCTTCCGCAATATCACGGGTGGCCAGCGCAATATCCTGATAACGATCCGCCACGCCCAGACGGCCGCAA 
               
               
                 TCAATAAAGCCGCTAAAACGGCCATTTTCCACCATAATGTTCGGCAGGCACGCATCACCATGGGTCACCACCAGA 
               
               
                 TCTTCGCCATCCGGCATGCTCGCTTTCAGACGCGCAAACAGCTCTGCCGGTGCCAGGCCCTGATGTTCTTCATCC 
               
               
                 AGATCATCCTGATCCACCAGGCCCGCTTCCATACGGGTACGCGCACGTTCAATACGATGTTTCGCCTGATGATCA 
               
               
                 AACGGACAGGTCGCCGGGTCCAGGGTATGCAGACGACGCATGGCATCCGCCATAATGCTCACTTTTTCTGCCGGC 
               
               
                 GCCAGATGGCTAGACAGCAGATCCTGACCCGGCACTTCGCCCAGCAGCAGCCAATCACGGCCCGCTTCGGTCACC 
               
               
                 ACATCCAGCACCGCCGCACACGGAACACCGGTGGTGGCCAGCCAGCTCAGACGCGCCGCTTCATCCTGCAGCTCG 
               
               
                 TTCAGCGCACCGCTCAGATCGGTTTTCACAAACAGCACCGGACGACCCTGCGCGCTCAGACGAAACACCGCCGCA 
               
               
                 TCAGAGCAGCCAATGGTCTGCTGCGCCCAATCATAGCCAAACAGACGTTCCACCCACGCTGCCGGGCTACCCGCA 
               
               
                 TGCAGGCCATCCTGTTCAATCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATG 
               
               
                 AGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCA 
               
               
                 CCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAAT 
               
               
                 AGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGGCCGCTACAGGGCGC 
               
               
                 TCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCT 
               
               
                 GGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACACGCGTAATACGA 
               
               
                 CTCACTATAG 
               
               
                   
               
               
                 VZV gB (SEQ ID NO: 68): 
               
               
                 MFVTAVVSVSPSSFYESLQVEPTQSEDITRSAHLGDGDEIREAIHKSQDAETKPTFYVCPPP 
               
               
                 TGSTIVRLEPPRTCPDYHLGKNFTEGIAVVYKENIAAYKFKATVYYKDVIVSTAWAGSSYTQ 
               
               
                 ITNRYADRVPIPVSEITDTIDKFGKCSSKATYVRNNHKVEAFNEDKNPQDMPLIASKYNSVG 
               
               
                 SKAWHTTNDTYMVAGTPGTYRTGTSVNCIIEEVEARSIFPYDSFGLSTGDIIYMSPFFGLRD 
               
               
                 GAYREHSNYAMDRFHQFEGYRQRDLDTRALLEPAARNFLVTPHLTVGWNWKPKRTEVCSLVK 
               
               
                 WREVEDVVRDEYAHNFRFTMKTLSTTFISETNEFNLNQIHLSQCVKEEARAIINRIYTTRYN 
               
               
                 SSHVRTGDIQTYLARGGFVVVFQPLLSNSLARLYLQELVRENTNHSPQKHPTRNTRSRRSVP 
               
               
                 VELRANRTITTTSSVEFAMLQFTYDHIQEHVNEMLARISSSWCQLQNRERALWSGLFPINPS 
               
               
                 ALASTILDQRVKARILGDVISVSNCPELGSDTRIILQNSMRVSGSTTRCYSRPLISIVSLNG 
               
               
                 SGTVEGQLGTDNELIMSRDLLEPCVANHKRYFLFGHHYVYYEDYRYVREIAVHDVGMISTYV 
               
               
                 DLNLTLLKDREFMPLQVYTRDELRDTGLLDYSEIQRRNQMHSLRFYDIDKVVQYDSGTAIMQ 
               
               
                 GMAQFFQGLGTAGQAVGHVVLGATGALLSTVHGFTTFLSNPFGALAVGLLVLAGLVAAFFAY 
               
               
                 RYVLKLKTSPMKALYPLTTKGLKQLPEGMDPFAEKPNATDTPIEEIGDSQNTEPSVNSGFDP 
               
               
                 DKFREAQEMIKYMTLVSAAERQESKARKKNKTSALLTSRLTGLALRNRRGYSRVRTENVTGV 
               
               
                   
               
               
                 VZV gH (SEQ ID NO: 69): 
               
               
                 MFALVLAVVILPLWTTANKSYVTPTPATRSIGHMSALLREYSDRNMSLKLEAFYPTGFDEEL 
               
               
                 IKSLHWGNDRKHVFLVIVKVNPTTHEGDVGLVIFPKYLLSPYHFKAEHRAPFPAGRFGFLSH 
               
               
                 PVTPDVSFFDSSFAPYLTTQHLVAFTTFPPNPLVWHLERAETAATAERPFGVSLLPARPTVP 
               
               
                 KNTILEHKAHFATWDALARHTFFSAEAIITNSTLRIHVPLFGSVWPIRYWATGSVLLTSDSG 
               
               
                 RVEVNIGVGFMSSLISLSSGLPIELIVVPHTVKLNAVTSDTTWFQLNPPGPDPGPSYRVYLL 
               
               
                 GRGLDMNFSKHATVDICAYPEESLDYRYHLSMAHTEALRMTTKADQHDINEESYYHIAARIA 
               
               
                 TSIFALSEMGRTTEYFLLDEIVDVQYQLKFLNYILMRIGAGAHPNTISGTSDLIFADPSQLH 
               
               
                 DELSLLFGQVKPANVDYFISYDEARDQLKTAYALSRGQDHVNALSLARRVIMSIYKGLLVKQ 
               
               
                 NLNATERQALFFASMILLNFREGLENSSRVLDGRTTLLLMTSMCTAAHATQAALNIQEGLAY 
               
               
                 LNPSKHMFTIPNVYSPCMGSLRTDLTEEIHVMNLLSAIPTRPGLNEVLHTQLDESEIFDAAF 
               
               
                 KTMMIFTTWTAKDLHILHTHVPEVFTCQDAAARNGEYVLILPAVQGHSYVITRNKPQRGLVY 
               
               
                 SLADVDVYNPISVVYLSKDTCVSEHGVIETVALPHPDNLKECLYCGSVFLRYLTTGAIMDII 
               
               
                 IIDSKDTERQLAAMGNSTIPPFNPDMHGDDSKAVLLFPNGTVVTLLGFERRQAIRMSGQYLG 
               
               
                 ASLGGAFLAVVGFGIIGWMLCGNSRLREYNKIPLT 
               
               
                   
               
               
                 VZV gL (SEQ ID NO: 70): 
               
               
                 MASHKWLLQMIVFLKTITIAYCLHLQDDTPLFFGAKPLSDVSLIITEPCVSSVYEAWDYAAP 
               
               
                 PVSNLSEALSGIVVKTKCPVPEVILWFKDKQMAYWTNPYVTLKGLTQSVGEEHKSGDIRDAL 
               
               
                 LDALSGVWVDSTPSSTNIPENGCVWGADRLFQRVCQ 
               
               
                   
               
               
                 VZV gI (SEQ ID NO: 71): 
               
               
                 MFLIQCLISAVIFYIQVTNALIFKGDHVSLQVNSSLTSILIPMQNDNYTEIKGQLVFIGEQL 
               
               
                 PTGTNYSGTLELLYADTVAFCFRSVQVIRYDGCPRIRTSAFISCRYKHSWHYGNSTDRISTE 
               
               
                 PDAGVMLKITKPGINDAGVYVLLVRLDHSRSTDGFILGVNVYTAGSHHNIHGVIYTSPSLQN 
               
               
                 GYSTRALFQQARLCDLPATPKGSGTSLFQHMLDLRAGKSLEDNPWLHEDVVTTETKSVVKEG 
               
               
                 IENHVYPTDMSTLPEKSLNDPPENLLIIIPIVASVMILTAMVIVIVISVKRRRIKKHPIYRP 
               
               
                 NTKTRRGIQNATPESDVMLEAAIAQLATIREESPPHSVVNPFVK 
               
               
                   
               
               
                 VZV gE (SEQ ID NO: 72): 
               
               
                 MGTVNKPVVGVLMGFGIITGTLRITNPVRASVLRYDDFHIDEDKLDTNSVYEPYYHSDHAES 
               
               
                 SWVNRGESSRKAYDHNSPYIWPRNDYDGFLENAHEHHGVYNQGRGIDSGERLMQPTQMSAQE 
               
               
                 DLGDDTGIHVIPTLNGDDRHKIVNVDQRQYGDVFKGDLNPKPQGQRLIEVSVEENHPFTLRA 
               
               
                 PIQRIYGVRYTETWSFLPSLTCTGDAAPAIQHICLKHTTCFQDVVVDVDCAENTKEDQLAEI 
               
               
                 SYRFQGKKEADQPWIVVNTSTLFDELELDPPEIEPGVLKVLRTEKQYLGVYIWNMRGSDGTS 
               
               
                 TYATFLVTWKGDEKTRNPTPAVTPQPRGAEFHMWNYHSHVFSVGDTFSLAMHLQYKIHEAPF 
               
               
                 DLLLEWLYVPIDPTCQPMRLYSTCLYHPNAPQCLSHMNSGCTFTSPHLAQRVASTVYQNCEH 
               
               
                 ADNYTAYCLGISHMEPSFGLILHDGGTTLKFVDTPESLSGLYVFVVYFNGHVEAVAYTVVST 
               
               
                 VDHFVNAIEERGFPPTAGQPPATTKPKEITPVNPGTSPLLRYAAWTGGLAAVVLLCLVIFLI 
               
               
                 CTAKRMRVKAYRVDKSPYNQSMYYAGLPVDDFEDSESTDTEEEFGNAIGGSHGGSSYTVYID 
               
               
                 KTR 
               
               
                   
               
               
                 VZV VEERep.SGPgB (SEQ ID NO: 73): 
               
               
                 1_ 
               
               
                 ataggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcacgttgacatc 
               
               
                 gaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttgaggtagaagccaagca 
               
               
                 ggtcactgataatgaccatgctaatgccagagcgttttcgcatctggcttcaaaactgatcgaaacgg 
               
               
                 aggtggacccatccgacacgatccttgacattggaagtgcgcccgcccgcagaatgtattctaagcac 
               
               
                 aagtatcattgtatctgtccgatgagatgtgcggaagatccggacagattgtataagtatgcaactaa 
               
               
                 gctgaagaaaaactgtaaggaaataactgataaggaattggacaagaaaatgaaggagctcgccgccg 
               
               
                 tcatgagcgaccctgacctggaaactgagactatgtgcctccacgacgacgagtcgtgtcgctacgaa 
               
               
                 gggcaagtcgctgtttaccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaa 
               
               
                 taagggagttagagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctg 
               
               
                 gagcatatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggccta 
               
               
                 tgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatttgaaacc 
               
               
                 atccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggacttactgaggagct 
               
               
                 ggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatgtcggtgtgagactatagtt 
               
               
                 agttgcgacgggtacgtcgttaaaagaatagctatcagtccaggcctgtatgggaagccttcaggcta 
               
               
                 tgctgctacgatgcaccgcgagggattcttgtgctgcaaagtgacagacacattgaacggggagaggg 
               
               
                 tctcttttcccgtgtgcacgtatgtgccagctacattgtgtgaccaaatgactggcatactggcaaca 
               
               
                 gatgtcagtgcggacgacgcgcaaaaactgctggttgggctcaaccagcgtatagtcgtcaacggtcg 
               
               
                 cacccagagaaacaccaataccatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggt 
               
               
                 gggcaaaggaatataaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtc 
               
               
                 atggggtgttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaac 
               
               
                 catcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacattggaga 
               
               
                 tcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctctcattaccgcc 
               
               
                 gaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgtgaagccgaggagttgcg 
               
               
                 cgcagctctaccacctttggcagctgatgttgaggagcccactctggaagccgatgtagacttgatgt 
               
               
                 tacaagaggctggggccggctcagtggagacacctcgtggcttgataaaggttaccagctacgatggc 
               
               
                 gaggacaagatcggctcttacgctgtgctttctccgcaggctgtactcaagagtgaaaaattatcttg 
               
               
                 catccaccctctcgctgaacaagtcatagtgataacacactctggccgaaaagggcgttatgccgtgg 
               
               
                 aaccataccatggtaaagtagtggtgccagagggacatgcaatacccgtccaggactttcaagctctg 
               
               
                 agtgaaagtgccaccattgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccac 
               
               
                 acatggaggagcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcg 
               
               
                 aatacctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctcaca 
               
               
                 ggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgaccagccgctcc 
               
               
                 ttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctggcatcattaaaagcg 
               
               
                 cagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgtgcagaaattataagggacgtc 
               
               
                 aagaaaatgaaagggctggacgtcaatgccagaactgtggactcagtgctcttgaatggatgcaaaca 
               
               
                 ccccgtagagaccctgtatattgacgaagcttttgcttgtcatgcaggtactctcagagcgctcatag 
               
               
                 ccattataagacctaaaaaggcagtgctctgcggggatcccaaacagtgcggtttttttaacatgatg 
               
               
                 tgcctgaaagtgcattttaaccacgagatttgcacacaagtcttccacaaaagcatctctcgccgttg 
               
               
                 cactaaatctgtgacttcggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccga 
               
               
                 aagagactaagattgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcact 
               
               
                 tgtttcagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctgc 
               
               
                 ctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcctctgtacg 
               
               
                 cacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgtgtggaaaacacta 
               
               
                 gccggcgacccatggataaaaacactgactgccaagtaccctgggaatttcactgccacgatagagga 
               
               
                 gtggcaagcagagcatgatgccatcatgaggcacatcttggagagaccggaccctaccgacgtcttcc 
               
               
                 agaataaggcaaacgtgtgttgggccaaggctttagtgccggtgctgaagaccgctggcatagacatg 
               
               
                 accactgaacaatggaacactgtggattattttgaaacggacaaagctcactcagcagagatagtatt 
               
               
                 gaaccaactatgcgtgaggttctttggactcgatctggactccggtctattttctgcacccactgttc 
               
               
                 cgttatccattaggaataatcactgggataactccccgtcgcctaacatgtacgggctgaataaagaa 
               
               
                 gtggtccgtcagctctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatga 
               
               
                 catgaacactggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgc 
               
               
                 ctcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagcaaattg 
               
               
                 aagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggttgactggttgtc 
               
               
                 agaccggcctgaggctaccttcagagctcggctggatttaggcatcccaggtgatgtgcccaaatatg 
               
               
                 acataatatttgttaatgtgaggaccccatataaataccatcactatcagcagtgtgaagaccatgcc 
               
               
                 attaagcttagcatgttgaccaagaaagcttgtctgcatctgaatcccggcggaacctgtgtcagcat 
               
               
                 aggttatggttacgctgacagggccagcgaaagcatcattggtgctatagcgcggcagttcaagtttt 
               
               
                 cccgggtatgcaaaccgaaatcctcacttgaagagacggaagttctgtttgtattcattgggtacgat 
               
               
                 cgcaaggcccgtacgcacaatccttacaagctttcatcaaccttgaccaacatttatacaggttccag 
               
               
                 actccacgaagccggatgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaag 
               
               
                 gagtgattataaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataag 
               
               
                 aaattcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgcagc 
               
               
                 taaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtgacaaacagt 
               
               
                 tggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaagtcagtagcgattcca 
               
               
                 ctgttgtccaccggcatcttttccgggaacaaagatcgactaacccaatcattgaaccatttgctgac 
               
               
                 agctttagacaccactgatgcagatgtagccatatactgcagggacaagaaatgggaaatgactctca 
               
               
                 aggaagcagtggctaggagagaagcagtggaggagatatgcatatccgacgactcttcagtgacagaa 
               
               
                 cctgatgcagagctggtgagggtgcatccgaagagttctttggctggaaggaagggctacagcacaag 
               
               
                 cgatggcaaaactttctcatatttggaagggaccaagtttcaccaggcggccaaggatatagcagaaa 
               
               
                 ttaatgccatgtggcccgttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagc 
               
               
                 atgagcagtattaggtcgaaatgccccgtcgaagagtcggaagcctccacaccacctagcacgctgcc 
               
               
                 ttgcttgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaaa 
               
               
                 ttactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatccaatgctcc 
               
               
                 cagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatctcgtggaaacacc 
               
               
                 accggtagacgagactccggagccatcggcagagaaccaatccacagaggggacacctgaacaaccac 
               
               
                 cacttataaccgaggatgagaccaggactagaacgcctgagccgatcatcatcgaagaggaagaagag 
               
               
                 gatagcataagtttgctgtcagatggcccgacccaccaggtgctgcaagtcgaggcagacattcacgg 
               
               
                 gccgccctctgtatctagctcatcctggtccattcctcatgcatccgactttgatgtggacagtttat 
               
               
                 ccatacttgacaccctggagggagctagcgtgaccagcggggcaacgtcagccgagactaactcttac 
               
               
                 ttcgcaaagagtatggagtttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctcc 
               
               
                 acatcccgctccgcgcacaagaacaccgtcacttgcacccagcagggcctgctcgagaaccagcctag 
               
               
                 tttccaccccgccaggcgtgaatagggtgatcactagagaggagctcgaggcgcttaccccgtcacgc 
               
               
                 actcctagcaggtcggtctcgagaaccagcctggtctccaacccgccaggcgtaaatagggtgattac 
               
               
                 aagagaggagtttgaggcgttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatctttt 
               
               
                 cctccgacaccggtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtg 
               
               
                 ttggagaggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtggagaaca 
               
               
                 tgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggcagaaggaaaagtg 
               
               
                 gagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaaccgtgccttttcaagccc 
               
               
                 caaggtcgcagtggaagcctgtaacgccatgttgaaagagaactttccgactgtggcttcttactgta 
               
               
                 ttattccagagtacgatgcctatttggacatggttgacggagcttcatgctgcttagacactgccagt 
               
               
                 ttttgccctgcaaagctgcgcagctttccaaagaaacactcctatttggaacccacaatacgatcggc 
               
               
                 agtgccttcagcgatccagaacacgctccagaacgtcctggcagctgccacaaaaagaaattgcaatg 
               
               
                 tcacgcaaatgagagaattgcccgtattggattcggcggcctttaatgtggaatgcttcaagaaatat 
               
               
                 gcgtgtaataatgaatattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggt 
               
               
                 aaattacattaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaata 
               
               
                 tgttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactccagga 
               
               
                 acaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctagcaacagcgta 
               
               
                 tctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgcttccgaacattcatacac 
               
               
                 tgtttgatatgtcggctgaagactttgacgctattatagccgagcacttccagcctggggattgtgtt 
               
               
                 ctggaaactgacatcgcgtcgtttgataaaagtgaggacgacgccatggctctgaccgcgttaatgat 
               
               
                 tctggaagacttaggtgtggacgcagagctgttgacgctgattgaggcggctttcggcgaaatttcat 
               
               
                 caatacatttgcccactaaaactaaatttaaattcggagccatgatgaaatctggaatgttcctcaca 
               
               
                 ctgtttgtgaacacagtcattaacattgtaatcgcaagcagagtgttgagagaacggctaaccggatc 
               
               
                 accatgtgcagcattcattggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcag 
               
               
                 acaggtgcgccacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgcct 
               
               
                 tatttctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagaccccct 
               
               
                 aaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacaggagaaggg 
               
               
                 cattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgtgcaaggcagtagaa 
               
               
                 tcaaggtatgaaaccgtaggaacttccatcatagttatggccatgactactctagctagcagtgttaa 
               
               
                 atcattcagctacctgagaggggcccctataactctctacggctaacctgaatggactacgacatagt 
               
               
                 ctagtcgagtctagtcgacgccaccatgttcgtgaccgccgtggtgtccgtgtcccccagcagctttt 
               
               
                 acgagagcctgcaggtcgagcccacccagagcgaggacatcacaagatctgcccacctgggcgacggc 
               
               
                 gacgagatcagagaggccatccacaagagccaggacgccgagacaaagcccaccttctacgtgtgccc 
               
               
                 cccacctaccggctctacaattgtgcggctggaaccccccagaacctgccctgattaccacctgggca 
               
               
                 agaacttcaccgagggaattgccgtggtgtacaaagagaatatcgccgcctacaagttcaaggccacc 
               
               
                 gtgtactacaaggacgtgatcgtgtccaccgcctgggccggcagcagctacacccagatcaccaacag 
               
               
                 atacgccgaccgggtgcccatccccgtgtctgagatcaccgacaccatcgacaagttcggcaagtgca 
               
               
                 gcagcaaggccacctacgtgcggaacaaccacaaggtggaagccttcaacgaggacaagaacccccag 
               
               
                 gacatgcccctgatcgccagcaagtacaacagcgtgggctccaaggcctggcacaccaccaacgacac 
               
               
                 ctacatggtggccggcacccccggcacatacagaacaggcaccagcgtgaactgcatcatcgaggaag 
               
               
                 tggaagcccggtccatcttcccatacgacagcttcggcctgagcaccggcgacattatctacatgagc 
               
               
                 cctttcttcggcctgcgggacggcgcctacagagagcacagcaactacgccatggaccggttccacca 
               
               
                 gttcgagggctacagacagcgggacctggacacaagagccctgctggaacctgccgccagaaacttcc 
               
               
                 tggtcacccctcacctgaccgtgggctggaactggaagcccaagcggaccgaagtgtgcagcctggtc 
               
               
                 aagtggcgcgaggtggaagatgtcgtgcgggatgagtacgcccacaacttccggttcaccatgaagac 
               
               
                 cctgagcaccaccttcatcagcgagacaaacgagttcaacctgaaccagatccacctgagccagtgcg 
               
               
                 tgaaagaggaagccagagccatcatcaaccggatctacaccacccggtacaacagcagccacgtgcgg 
               
               
                 accggcgatatccagacctatctggctagaggcggcttcgtggtggtgtttcagcccctgctgagcaa 
               
               
                 cagcctggctagactgtacctgcaggaactcgtcagagagaacaccaaccacagcccccagaagcacc 
               
               
                 ccacccggaataccagatccagacgcagcgtgcccgtggaactgagagccaaccggaccatcaccacc 
               
               
                 accagcagcgtggaattcgccatgctgcagttcacctacgaccacatccaggaacacgtgaacgagat 
               
               
                 gctggcccggatcagcagcagttggtgccagctgcagaatcgggaaagggccctgtggtccggcctgt 
               
               
                 tccccatcaatccaagcgccctggccagcaccatcctggaccagagagtgaaggccagaatcctgggg 
               
               
                 gacgtgatcagcgtgtccaactgtcctgagctgggcagcgacacccggatcatcctgcagaacagcat 
               
               
                 gcgggtgtccggcagcaccaccagatgctacagcagacccctgatcagcatcgtgtccctgaacggca 
               
               
                 gcggcacagtggaaggccagctgggcaccgataacgagctgatcatgagccgggacctgctcgaaccc 
               
               
                 tgcgtggccaatcacaagcggtactttctgttcggccaccactacgtgtactatgaggactacagata 
               
               
                 cgtgcgcgagatcgccgtgcacgacgtgggcatgatcagcacctacgtggacctgaacctgaccctgc 
               
               
                 tgaaggaccgcgagttcatgccactgcaggtctacacccgggacgagctgagagataccggcctgctg 
               
               
                 gactacagcgagatccagcggcggaaccagatgcactccctgcggttctacgacatcgacaaggtggt 
               
               
                 gcagtacgacagcggcaccgccatcatgcagggcatggcccagttctttcagggcctgggaacagccg 
               
               
                 gacaggccgtgggacatgtggtgctgggagctacaggcgccctgctgtctaccgtgcacggcttcacc 
               
               
                 acctttctgagcaaccccttcggagccctggctgtgggactgctggtcctggctggactggtggccgc 
               
               
                 cttctttgcctaccgctacgtgctgaagctgaaaaccagccccatgaaggccctgtaccccctgacca 
               
               
                 ccaagggcctgaagcagctgcctgagggcatggaccccttcgccgagaagcccaatgccaccgacacc 
               
               
                 cccatcgaggaaatcggcgacagccagaacaccgagccctccgtgaacagcggcttcgaccccgacaa 
               
               
                 gtttcgcgaggcccaggaaatgatcaagtacatgaccctggtgtctgctgccgagcggcaggaaagca 
               
               
                 aggcccggaagaagaacaagacctccgccctgctgaccagcagactgacaggactggccctgcggaac 
               
               
                 agacggggctatagcagagtgcggaccgagaatgtgaccggcgtgtaatctagacgcggccgcataca 
               
               
                 gcagcaattggcaagctgcttacatagaactcgcggcgattggcatgccgccttaaaatttttatttt 
               
               
                 atttttcttttcttttccgaatcggattttgtttttaatatttcaaaaaaaaaaaaaaaaaaaaaaaa 
               
               
                 aaaaaaaaaaagggtcggcatggcatctccacctcctcgcggtccgacctgggcatccgaaggaggac 
               
               
                 gcacgtccactcggatggctaagggagagccacgtttaaaccagctccaattcgccctatagtgagtc 
               
               
                 gtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaac 
               
               
                 ttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgc 
               
               
                 ccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcgcattaagcgcggc 
               
               
                 gggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctt 
               
               
                 tcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctcccttta 
               
               
                 gggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtag 
               
               
                 tgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggac 
               
               
                 tcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttg 
               
               
                 ccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaat 
               
               
                 attaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttattttt 
               
               
                 ctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaa 
               
               
                 aaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgcctt 
               
               
                 cctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagt 
               
               
                 gggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttc 
               
               
                 caatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagag 
               
               
                 caactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagca 
               
               
                 tcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcgg 
               
               
                 ccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggat 
               
               
                 catgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacac 
               
               
                 cacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagctt 
               
               
                 cccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggccctt 
               
               
                 ccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagc 
               
               
                 actggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatgg 
               
               
                 atgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaa 
               
               
                 gtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagat 
               
               
                 cctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccg 
               
               
                 tagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaa 
               
               
                 aaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaac 
               
               
                 tggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttca 
               
               
                 agaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggc 
               
               
                 gataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctg 
               
               
                 aacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagc 
               
               
                 gtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagg 
               
               
                 gtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgg 
               
               
                 gtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaa 
               
               
                 acgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcct 
               
               
                 gcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcag 
               
               
                 ccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctc 
               
               
                 tccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagt 
               
               
                 gagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgctccc 
               
               
                 ggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgatt 
               
               
                 acgccaagcgcgcaattaaccctcactaaagggaacaaaagctgggtaccgggcccacgcgtaatacg 
               
               
                 actcactatag_13339 
               
               
                   
               
               
                 VZV VEERep.SGPgH (SEQ ID NO: 74): 
               
               
                 1_ 
               
               
                 ataggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcacgttgacatc 
               
               
                 gaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttgaggtagaagccaagca 
               
               
                 ggtcactgataatgaccatgctaatgccagagcgttttcgcatctggcttcaaaactgatcgaaacgg 
               
               
                 aggtggacccatccgacacgatccttgacattggaagtgcgcccgcccgcagaatgtattctaagcac 
               
               
                 aagtatcattgtatctgtccgatgagatgtgcggaagatccggacagattgtataagtatgcaactaa 
               
               
                 gctgaagaaaaactgtaaggaaataactgataaggaattggacaagaaaatgaaggagctcgccgccg 
               
               
                 tcatgagcgaccctgacctggaaactgagactatgtgcctccacgacgacgagtcgtgtcgctacgaa 
               
               
                 gggcaagtcgctgtttaccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaa 
               
               
                 taagggagttagagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctg 
               
               
                 gagcatatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggccta 
               
               
                 tgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatttgaaacc 
               
               
                 atccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggacttactgaggagct 
               
               
                 ggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatgtcggtgtgagactatagtt 
               
               
                 agttgcgacgggtacgtcgttaaaagaatagctatcagtccaggcctgtatgggaagccttcaggcta 
               
               
                 tgctgctacgatgcaccgcgagggattcttgtgctgcaaagtgacagacacattgaacggggagaggg 
               
               
                 tctcttttcccgtgtgcacgtatgtgccagctacattgtgtgaccaaatgactggcatactggcaaca 
               
               
                 gatgtcagtgcggacgacgcgcaaaaactgctggttgggctcaaccagcgtatagtcgtcaacggtcg 
               
               
                 cacccagagaaacaccaataccatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggt 
               
               
                 gggcaaaggaatataaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtc 
               
               
                 atggggtgttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaac 
               
               
                 catcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacattggaga 
               
               
                 tcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctctcattaccgcc 
               
               
                 gaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgtgaagccgaggagttgcg 
               
               
                 cgcagctctaccacctttggcagctgatgttgaggagcccactctggaagccgatgtagacttgatgt 
               
               
                 tacaagaggctggggccggctcagtggagacacctcgtggcttgataaaggttaccagctacgatggc 
               
               
                 gaggacaagatcggctcttacgctgtgctttctccgcaggctgtactcaagagtgaaaaattatcttg 
               
               
                 catccaccctctcgctgaacaagtcatagtgataacacactctggccgaaaagggcgttatgccgtgg 
               
               
                 aaccataccatggtaaagtagtggtgccagagggacatgcaatacccgtccaggactttcaagctctg 
               
               
                 agtgaaagtgccaccattgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccac 
               
               
                 acatggaggagcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcg 
               
               
                 aatacctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctcaca 
               
               
                 ggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgaccagccgctcc 
               
               
                 ttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctggcatcattaaaagcg 
               
               
                 cagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgtgcagaaattataagggacgtc 
               
               
                 aagaaaatgaaagggctggacgtcaatgccagaactgtggactcagtgctcttgaatggatgcaaaca 
               
               
                 ccccgtagagaccctgtatattgacgaagcttttgcttgtcatgcaggtactctcagagcgctcatag 
               
               
                 ccattataagacctaaaaaggcagtgctctgcggggatcccaaacagtgcggtttttttaacatgatg 
               
               
                 tgcctgaaagtgcattttaaccacgagatttgcacacaagtcttccacaaaagcatctctcgccgttg 
               
               
                 cactaaatctgtgacttcggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccga 
               
               
                 aagagactaagattgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcact 
               
               
                 tgtttcagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctgc 
               
               
                 ctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcctctgtacg 
               
               
                 cacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgtgtggaaaacacta 
               
               
                 gccggcgacccatggataaaaacactgactgccaagtaccctgggaatttcactgccacgatagagga 
               
               
                 gtggcaagcagagcatgatgccatcatgaggcacatcttggagagaccggaccctaccgacgtcttcc 
               
               
                 agaataaggcaaacgtgtgttgggccaaggctttagtgccggtgctgaagaccgctggcatagacatg 
               
               
                 accactgaacaatggaacactgtggattattttgaaacggacaaagctcactcagcagagatagtatt 
               
               
                 gaaccaactatgcgtgaggttctttggactcgatctggactccggtctattttctgcacccactgttc 
               
               
                 cgttatccattaggaataatcactgggataactccccgtcgcctaacatgtacgggctgaataaagaa 
               
               
                 gtggtccgtcagctctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatga 
               
               
                 catgaacactggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgc 
               
               
                 ctcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagcaaattg 
               
               
                 aagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggttgactggttgtc 
               
               
                 agaccggcctgaggctaccttcagagctcggctggatttaggcatcccaggtgatgtgcccaaatatg 
               
               
                 acataatatttgttaatgtgaggaccccatataaataccatcactatcagcagtgtgaagaccatgcc 
               
               
                 attaagcttagcatgttgaccaagaaagcttgtctgcatctgaatcccggcggaacctgtgtcagcat 
               
               
                 aggttatggttacgctgacagggccagcgaaagcatcattggtgctatagcgcggcagttcaagtttt 
               
               
                 cccgggtatgcaaaccgaaatcctcacttgaagagacggaagttctgtttgtattcattgggtacgat 
               
               
                 cgcaaggcccgtacgcacaatccttacaagctttcatcaaccttgaccaacatttatacaggttccag 
               
               
                 actccacgaagccggatgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaag 
               
               
                 gagtgattataaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataag 
               
               
                 aaattcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgcagc 
               
               
                 taaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtgacaaacagt 
               
               
                 tggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaagtcagtagcgattcca 
               
               
                 ctgttgtccaccggcatcttttccgggaacaaagatcgactaacccaatcattgaaccatttgctgac 
               
               
                 agctttagacaccactgatgcagatgtagccatatactgcagggacaagaaatgggaaatgactctca 
               
               
                 aggaagcagtggctaggagagaagcagtggaggagatatgcatatccgacgactcttcagtgacagaa 
               
               
                 cctgatgcagagctggtgagggtgcatccgaagagttctttggctggaaggaagggctacagcacaag 
               
               
                 cgatggcaaaactttctcatatttggaagggaccaagtttcaccaggcggccaaggatatagcagaaa 
               
               
                 ttaatgccatgtggcccgttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagc 
               
               
                 atgagcagtattaggtcgaaatgccccgtcgaagagtcggaagcctccacaccacctagcacgctgcc 
               
               
                 ttgcttgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaaa 
               
               
                 ttactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatccaatgctcc 
               
               
                 cagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatctcgtggaaacacc 
               
               
                 accggtagacgagactccggagccatcggcagagaaccaatccacagaggggacacctgaacaaccac 
               
               
                 cacttataaccgaggatgagaccaggactagaacgcctgagccgatcatcatcgaagaggaagaagag 
               
               
                 gatagcataagtttgctgtcagatggcccgacccaccaggtgctgcaagtcgaggcagacattcacgg 
               
               
                 gccgccctctgtatctagctcatcctggtccattcctcatgcatccgactttgatgtggacagtttat 
               
               
                 ccatacttgacaccctggagggagctagcgtgaccagcggggcaacgtcagccgagactaactcttac 
               
               
                 ttcgcaaagagtatggagtttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctcc 
               
               
                 acatcccgctccgcgcacaagaacaccgtcacttgcacccagcagggcctgctcgagaaccagcctag 
               
               
                 tttccaccccgccaggcgtgaatagggtgatcactagagaggagctcgaggcgcttaccccgtcacgc 
               
               
                 actcctagcaggtcggtctcgagaaccagcctggtctccaacccgccaggcgtaaatagggtgattac 
               
               
                 aagagaggagtttgaggcgttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatctttt 
               
               
                 cctccgacaccggtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtg 
               
               
                 ttggagaggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtggagaaca 
               
               
                 tgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggcagaaggaaaagtg 
               
               
                 gagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaaccgtgccttttcaagccc 
               
               
                 caaggtcgcagtggaagcctgtaacgccatgttgaaagagaactttccgactgtggcttcttactgta 
               
               
                 ttattccagagtacgatgcctatttggacatggttgacggagcttcatgctgcttagacactgccagt 
               
               
                 ttttgccctgcaaagctgcgcagctttccaaagaaacactcctatttggaacccacaatacgatcggc 
               
               
                 agtgccttcagcgatccagaacacgctccagaacgtcctggcagctgccacaaaaagaaattgcaatg 
               
               
                 tcacgcaaatgagagaattgcccgtattggattcggcggcctttaatgtggaatgcttcaagaaatat 
               
               
                 gcgtgtaataatgaatattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggt 
               
               
                 aaattacattaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaata 
               
               
                 tgttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactccagga 
               
               
                 acaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctagcaacagcgta 
               
               
                 tctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgcttccgaacattcatacac 
               
               
                 tgtttgatatgtcggctgaagactttgacgctattatagccgagcacttccagcctggggattgtgtt 
               
               
                 ctggaaactgacatcgcgtcgtttgataaaagtgaggacgacgccatggctctgaccgcgttaatgat 
               
               
                 tctggaagacttaggtgtggacgcagagctgttgacgctgattgaggcggctttcggcgaaatttcat 
               
               
                 caatacatttgcccactaaaactaaatttaaattcggagccatgatgaaatctggaatgttcctcaca 
               
               
                 ctgtttgtgaacacagtcattaacattgtaatcgcaagcagagtgttgagagaacggctaaccggatc 
               
               
                 accatgtgcagcattcattggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcag 
               
               
                 acaggtgcgccacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgcct 
               
               
                 tatttctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagaccccct 
               
               
                 aaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacaggagaaggg 
               
               
                 cattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgtgcaaggcagtagaa 
               
               
                 tcaaggtatgaaaccgtaggaacttccatcatagttatggccatgactactctagctagcagtgttaa 
               
               
                 atcattcagctacctgagaggggcccctataactctctacggctaacctgaatggactacgacatagt 
               
               
                 ctagtcgagtctagtcgacgccaccatgttcgccctggtgctggccgtggtcatcctgcctctgtgga 
               
               
                 ccaccgccaacaagagctacgtgacccccacacccgccaccagatccatcggacacatgagcgccctg 
               
               
                 ctgagagagtacagcgaccggaacatgagcctgaagctggaagccttctaccccaccggcttcgacga 
               
               
                 ggaactgatcaagagcctgcactggggcaacgaccggaagcacgtgttcctcgtgatcgtgaaagtga 
               
               
                 accccaccacccacgagggcgacgtcggcctggtcatcttccccaagtacctgctgagcccctaccac 
               
               
                 ttcaaggccgagcacagagcccccttccctgctggccgctttggctttctgagccaccctgtgacccc 
               
               
                 cgacgtgtcattcttcgacagcagcttcgccccctacctgaccacacagcacctggtggccttcacca 
               
               
                 ccttcccccccaatcctctcgtgtggcacctggaaagagccgagacagccgccaccgccgaaagacct 
               
               
                 tttggcgtgtccctgctgcccgccagacctaccgtgcccaagaacaccatcctggaacacaaggccca 
               
               
                 cttcgccacctgggatgccctggccagacacaccttctttagcgccgaggccatcatcaccaacagca 
               
               
                 ccctgagaatccacgtgcccctgttcggcagcgtgtggcccatcagatactgggccacaggcagcgtg 
               
               
                 ctgctgaccagcgatagcggcagagtggaagtgaacatcggcgtgggcttcatgagcagcctgatcag 
               
               
                 cctgagcagcggcctgcccatcgagctgattgtggtgccccacaccgtgaagctgaacgccgtgacca 
               
               
                 gcgacaccacctggttccagctgaacccccctggccctgatcctggccctagttacagagtgtacctg 
               
               
                 ctgggcagaggcctggacatgaacttcagcaagcacgccaccgtggacatctgcgcctaccctgagga 
               
               
                 aagcctggactacagataccacctgagcatggcccacaccgaggccctgagaatgaccaccaaggccg 
               
               
                 accagcacgacatcaacgaggaaagctactaccacattgccgccagaatcgccaccagcatcttcgcc 
               
               
                 ctgagcgagatgggccggaccaccgagtactttctgctggacgagatcgtggacgtgcagtaccagct 
               
               
                 gaagttcctgaactacatcctgatgcggatcggcgctggcgcccaccctaataccatcagcggcacca 
               
               
                 gcgacctgatcttcgccgatcctagccagctgcacgacgagctgagcctgctgttcggccaggtcaaa 
               
               
                 cccgccaacgtggactacttcatcagctacgacgaggcccgggaccagctgaaaacagcctacgccct 
               
               
                 gtccagaggccaggatcatgtgaacgccctgtccctggccaggcgcgtgatcatgagcatctacaagg 
               
               
                 gcctgctggtcaagcagaacctgaacgccaccgagcggcaggccctgttcttcgccagcatgatcctg 
               
               
                 ctgaacttcagagagggcctggaaaacagcagccgggtgctggatggcagaaccaccctgctgctgat 
               
               
                 gaccagcatgtgcacagccgcccatgccacacaggccgccctgaatatccaggaaggcctggcttacc 
               
               
                 tgaaccccagcaagcacatgttcaccatccccaacgtgtacagcccctgcatgggcagcctgagaacc 
               
               
                 gacctgaccgaagagatccacgtgatgaacctgctgtccgccatccccaccagacccggactgaatga 
               
               
                 ggtgctgcacacccagctggacgagtccgagatcttcgacgccgccttcaagaccatgatgatcttta 
               
               
                 ccacctggaccgccaaggacctgcacatcctgcacacacacgtgcccgaggtgttcacatgccaagat 
               
               
                 gccgccgctcggaacggcgagtatgtgctgattctgcctgccgtgcagggccacagctacgtgatcac 
               
               
                 ccggaacaagccccagcggggcctggtgtatagcctggctgacgtggacgtgtacaaccccatcagcg 
               
               
                 tggtgtacctgagcaaggatacctgcgtgtccgagcacggcgtgatcgaaacagtggccctgccccac 
               
               
                 cccgacaacctgaaagagtgcctgtactgcggctccgtgttcctgcggtatctgaccaccggcgccat 
               
               
                 catggacatcatcatcatcgacagcaaggacaccgagagacagctggccgccatgggcaacagcacca 
               
               
                 tcccccccttcaaccccgacatgcacggcgacgatagcaaggccgtgctgctgttccccaacggcacc 
               
               
                 gtggtcacactgctgggcttcgagcggagacaggccatcagaatgagcggccagtacctgggcgcctc 
               
               
                 tctgggtggtgcctttctggccgtcgtgggctttggcatcatcggctggatgctgtgcggcaacagca 
               
               
                 gactgcgcgagtacaacaagatccccctgacctaatctagacgcggccgcatacagcagcaattggca 
               
               
                 agctgcttacatagaactcgcggcgattggcatgccgccttaaaatttttattttatttttcttttct 
               
               
                 tttccgaatcggattttgtttttaatatttcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaagg 
               
               
                 gtcggcatggcatctccacctcctcgcggtccgacctgggcatccgaaggaggacgcacgtccactcg 
               
               
                 gatggctaagggagagccacgtttaaaccagctccaattcgccctatagtgagtcgtattacgcgcgc 
               
               
                 tcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgc 
               
               
                 agcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagt 
               
               
                 tgcgcagcctgaatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggtt 
               
               
                 acgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctt 
               
               
                 tctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgattta 
               
               
                 gtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccc 
               
               
                 tgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaac 
               
               
                 tggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcct 
               
               
                 attggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttaca 
               
               
                 atttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattca 
               
               
                 aatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtat 
               
               
                 gagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctc 
               
               
                 acccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaa 
               
               
                 ctggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcac 
               
               
                 ttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgcc 
               
               
                 gcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggc 
               
               
                 atgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttct 
               
               
                 gacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgcc 
               
               
                 ttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgta 
               
               
                 gcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaatt 
               
               
                 aatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggt 
               
               
                 ttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagat 
               
               
                 ggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatag 
               
               
                 acagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatata 
               
               
                 tactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataat 
               
               
                 ctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa 
               
               
                 aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctac 
               
               
                 cagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcaga 
               
               
                 gcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagc 
               
               
                 accgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtc 
               
               
                 ttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcg 
               
               
                 tgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgaga 
               
               
                 aagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggag 
               
               
                 agcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctc 
               
               
                 tgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgc 
               
               
                 ggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctg 
               
               
                 attctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgag 
               
               
                 cgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttg 
               
               
                 gccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa 
               
               
                 ttaatgtgagttagctcactcattaggcaccccaggctttacactttatgctcccggctcgtatgttg 
               
               
                 tgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcgc 
               
               
                 aattaaccctcactaaagggaacaaaagctgggtaccgggcccacgcgtaatacgactcactatag_1 
               
               
                 3258 
               
               
                   
               
               
                 VZV VEERep.SGPgL (SEQ ID NO: 75): 
               
               
                 1_ 
               
               
                 ataggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcacgttgacatc 
               
               
                 gaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttgaggtagaagccaagca 
               
               
                 ggtcactgataatgaccatgctaatgccagagcgttttcgcatctggcttcaaaactgatcgaaacgg 
               
               
                 aggtggacccatccgacacgatccttgacattggaagtgcgcccgcccgcagaatgtattctaagcac 
               
               
                 aagtatcattgtatctgtccgatgagatgtgcggaagatccggacagattgtataagtatgcaactaa 
               
               
                 gctgaagaaaaactgtaaggaaataactgataaggaattggacaagaaaatgaaggagctcgccgccg 
               
               
                 tcatgagcgaccctgacctggaaactgagactatgtgcctccacgacgacgagtcgtgtcgctacgaa 
               
               
                 gggcaagtcgctgtttaccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaa 
               
               
                 taagggagttagagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctg 
               
               
                 gagcatatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggccta 
               
               
                 tgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatttgaaacc 
               
               
                 atccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggacttactgaggagct 
               
               
                 ggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatgtcggtgtgagactatagtt 
               
               
                 agttgcgacgggtacgtcgttaaaagaatagctatcagtccaggcctgtatgggaagccttcaggcta 
               
               
                 tgctgctacgatgcaccgcgagggattcttgtgctgcaaagtgacagacacattgaacggggagaggg 
               
               
                 tctcttttcccgtgtgcacgtatgtgccagctacattgtgtgaccaaatgactggcatactggcaaca 
               
               
                 gatgtcagtgcggacgacgcgcaaaaactgctggttgggctcaaccagcgtatagtcgtcaacggtcg 
               
               
                 cacccagagaaacaccaataccatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggt 
               
               
                 gggcaaaggaatataaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtc 
               
               
                 atggggtgttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaac 
               
               
                 catcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacattggaga 
               
               
                 tcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctctcattaccgcc 
               
               
                 gaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgtgaagccgaggagttgcg 
               
               
                 cgcagctctaccacctttggcagctgatgttgaggagcccactctggaagccgatgtagacttgatgt 
               
               
                 tacaagaggctggggccggctcagtggagacacctcgtggcttgataaaggttaccagctacgatggc 
               
               
                 gaggacaagatcggctcttacgctgtgctttctccgcaggctgtactcaagagtgaaaaattatcttg 
               
               
                 catccaccctctcgctgaacaagtcatagtgataacacactctggccgaaaagggcgttatgccgtgg 
               
               
                 aaccataccatggtaaagtagtggtgccagagggacatgcaatacccgtccaggactttcaagctctg 
               
               
                 agtgaaagtgccaccattgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccac 
               
               
                 acatggaggagcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcg 
               
               
                 aatacctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctcaca 
               
               
                 ggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgaccagccgctcc 
               
               
                 ttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctggcatcattaaaagcg 
               
               
                 cagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgtgcagaaattataagggacgtc 
               
               
                 aagaaaatgaaagggctggacgtcaatgccagaactgtggactcagtgctcttgaatggatgcaaaca 
               
               
                 ccccgtagagaccctgtatattgacgaagcttttgcttgtcatgcaggtactctcagagcgctcatag 
               
               
                 ccattataagacctaaaaaggcagtgctctgcggggatcccaaacagtgcggtttttttaacatgatg 
               
               
                 tgcctgaaagtgcattttaaccacgagatttgcacacaagtcttccacaaaagcatctctcgccgttg 
               
               
                 cactaaatctgtgacttcggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccga 
               
               
                 aagagactaagattgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcact 
               
               
                 tgtttcagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctgc 
               
               
                 ctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcctctgtacg 
               
               
                 cacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgtgtggaaaacacta 
               
               
                 gccggcgacccatggataaaaacactgactgccaagtaccctgggaatttcactgccacgatagagga 
               
               
                 gtggcaagcagagcatgatgccatcatgaggcacatcttggagagaccggaccctaccgacgtcttcc 
               
               
                 agaataaggcaaacgtgtgttgggccaaggctttagtgccggtgctgaagaccgctggcatagacatg 
               
               
                 accactgaacaatggaacactgtggattattttgaaacggacaaagctcactcagcagagatagtatt 
               
               
                 gaaccaactatgcgtgaggttctttggactcgatctggactccggtctattttctgcacccactgttc 
               
               
                 cgttatccattaggaataatcactgggataactccccgtcgcctaacatgtacgggctgaataaagaa 
               
               
                 gtggtccgtcagctctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatga 
               
               
                 catgaacactggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgc 
               
               
                 ctcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagcaaattg 
               
               
                 aagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggttgactggttgtc 
               
               
                 agaccggcctgaggctaccttcagagctcggctggatttaggcatcccaggtgatgtgcccaaatatg 
               
               
                 acataatatttgttaatgtgaggaccccatataaataccatcactatcagcagtgtgaagaccatgcc 
               
               
                 attaagcttagcatgttgaccaagaaagcttgtctgcatctgaatcccggcggaacctgtgtcagcat 
               
               
                 aggttatggttacgctgacagggccagcgaaagcatcattggtgctatagcgcggcagttcaagtttt 
               
               
                 cccgggtatgcaaaccgaaatcctcacttgaagagacggaagttctgtttgtattcattgggtacgat 
               
               
                 cgcaaggcccgtacgcacaatccttacaagctttcatcaaccttgaccaacatttatacaggttccag 
               
               
                 actccacgaagccggatgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaag 
               
               
                 gagtgattataaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataag 
               
               
                 aaattcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgcagc 
               
               
                 taaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtgacaaacagt 
               
               
                 tggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaagtcagtagcgattcca 
               
               
                 ctgttgtccaccggcatcttttccgggaacaaagatcgactaacccaatcattgaaccatttgctgac 
               
               
                 agctttagacaccactgatgcagatgtagccatatactgcagggacaagaaatgggaaatgactctca 
               
               
                 aggaagcagtggctaggagagaagcagtggaggagatatgcatatccgacgactcttcagtgacagaa 
               
               
                 cctgatgcagagctggtgagggtgcatccgaagagttctttggctggaaggaagggctacagcacaag 
               
               
                 cgatggcaaaactttctcatatttggaagggaccaagtttcaccaggcggccaaggatatagcagaaa 
               
               
                 ttaatgccatgtggcccgttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagc 
               
               
                 atgagcagtattaggtcgaaatgccccgtcgaagagtcggaagcctccacaccacctagcacgctgcc 
               
               
                 ttgcttgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaaa 
               
               
                 ttactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatccaatgctcc 
               
               
                 cagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatctcgtggaaacacc 
               
               
                 accggtagacgagactccggagccatcggcagagaaccaatccacagaggggacacctgaacaaccac 
               
               
                 cacttataaccgaggatgagaccaggactagaacgcctgagccgatcatcatcgaagaggaagaagag 
               
               
                 gatagcataagtttgctgtcagatggcccgacccaccaggtgctgcaagtcgaggcagacattcacgg 
               
               
                 gccgccctctgtatctagctcatcctggtccattcctcatgcatccgactttgatgtggacagtttat 
               
               
                 ccatacttgacaccctggagggagctagcgtgaccagcggggcaacgtcagccgagactaactcttac 
               
               
                 ttcgcaaagagtatggagtttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctcc 
               
               
                 acatcccgctccgcgcacaagaacaccgtcacttgcacccagcagggcctgctcgagaaccagcctag 
               
               
                 tttccaccccgccaggcgtgaatagggtgatcactagagaggagctcgaggcgcttaccccgtcacgc 
               
               
                 actcctagcaggtcggtctcgagaaccagcctggtctccaacccgccaggcgtaaatagggtgattac 
               
               
                 aagagaggagtttgaggcgttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatctttt 
               
               
                 cctccgacaccggtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtg 
               
               
                 ttggagaggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtggagaaca 
               
               
                 tgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggcagaaggaaaagtg 
               
               
                 gagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaaccgtgccttttcaagccc 
               
               
                 caaggtcgcagtggaagcctgtaacgccatgttgaaagagaactttccgactgtggcttcttactgta 
               
               
                 ttattccagagtacgatgcctatttggacatggttgacggagcttcatgctgcttagacactgccagt 
               
               
                 ttttgccctgcaaagctgcgcagctttccaaagaaacactcctatttggaacccacaatacgatcggc 
               
               
                 agtgccttcagcgatccagaacacgctccagaacgtcctggcagctgccacaaaaagaaattgcaatg 
               
               
                 tcacgcaaatgagagaattgcccgtattggattcggcggcctttaatgtggaatgcttcaagaaatat 
               
               
                 gcgtgtaataatgaatattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggt 
               
               
                 aaattacattaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaata 
               
               
                 tgttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactccagga 
               
               
                 acaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctagcaacagcgta 
               
               
                 tctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgcttccgaacattcatacac 
               
               
                 tgtttgatatgtcggctgaagactttgacgctattatagccgagcacttccagcctggggattgtgtt 
               
               
                 ctggaaactgacatcgcgtcgtttgataaaagtgaggacgacgccatggctctgaccgcgttaatgat 
               
               
                 tctggaagacttaggtgtggacgcagagctgttgacgctgattgaggcggctttcggcgaaatttcat 
               
               
                 caatacatttgcccactaaaactaaatttaaattcggagccatgatgaaatctggaatgttcctcaca 
               
               
                 ctgtttgtgaacacagtcattaacattgtaatcgcaagcagagtgttgagagaacggctaaccggatc 
               
               
                 accatgtgcagcattcattggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcag 
               
               
                 acaggtgcgccacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgcct 
               
               
                 tatttctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagaccccct 
               
               
                 aaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacaggagaaggg 
               
               
                 cattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgtgcaaggcagtagaa 
               
               
                 tcaaggtatgaaaccgtaggaacttccatcatagttatggccatgactactctagctagcagtgttaa 
               
               
                 atcattcagctacctgagaggggcccctataactctctacggctaacctgaatggactacgacatagt 
               
               
                 ctagtcgagtctagtcgacgccaccatggccagccacaagtggctgctgcagatgatcgtgttcctga 
               
               
                 aaaccatcacaatcgcctactgcctgcatctgcaggacgacacccctctgttcttcggcgccaagcct 
               
               
                 ctgagcgacgtgtccctgatcatcaccgagccttgcgtgtccagcgtgtacgaggcctgggattatgc 
               
               
                 cgcccctcccgtgtccaatctgagcgaagccctgagcggcatcgtggtcaagaccaagtgccccgtgc 
               
               
                 ccgaagtgatcctgtggttcaaggacaagcagatggcctactggaccaacccttacgtgaccctgaag 
               
               
                 ggcctgacccagagcgtgggcgaggaacacaagagcggcgacatcagagatgccctgctggatgccct 
               
               
                 gtccggtgtctgggtggacagcacaccctccagcaccaacatccccgagaacggctgtgtgtggggag 
               
               
                 ccgaccggctgttccagagagtgtgtcagtaatctagacgcggccgcatacagcagcaattggcaagc 
               
               
                 tgcttacatagaactcgcggcgattggcatgccgccttaaaatttttattttatttttcttttctttt 
               
               
                 ccgaatcggattttgtttttaatatttcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaagggtc 
               
               
                 ggcatggcatctccacctcctcgcggtccgacctgggcatccgaaggaggacgcacgtccactcggat 
               
               
                 ggctaagggagagccacgtttaaaccagctccaattcgccctatagtgagtcgtattacgcgcgctca 
               
               
                 ctggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagc 
               
               
                 acatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgc 
               
               
                 gcagcctgaatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacg 
               
               
                 cgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttct 
               
               
                 cgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtg 
               
               
                 ctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctga 
               
               
                 tagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactgg 
               
               
                 aacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctatt 
               
               
                 ggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatt 
               
               
                 taggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaat 
               
               
                 atgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgag 
               
               
                 tattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacc 
               
               
                 cagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactg 
               
               
                 gatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttt 
               
               
                 taaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgca 
               
               
                 tacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatg 
               
               
                 acagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgac 
               
               
                 aacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttg 
               
               
                 atcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagca 
               
               
                 atggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaat 
               
               
                 agactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggttta 
               
               
                 ttgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggt 
               
               
                 aagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagaca 
               
               
                 gatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatac 
               
               
                 tttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctc 
               
               
                 atgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaagg 
               
               
                 atcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccag 
               
               
                 cggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcg 
               
               
                 cagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcacc 
               
               
                 gcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtctta 
               
               
                 ccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgc 
               
               
                 acacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaag 
               
               
                 cgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagc 
               
               
                 gcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctga 
               
               
                 cttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggc 
               
               
                 ctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgatt 
               
               
                 ctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgc 
               
               
                 agcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggcc 
               
               
                 gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaatta 
               
               
                 atgtgagttagctcactcattaggcaccccaggctttacactttatgctcccggctcgtatgttgtgt 
               
               
                 ggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcgcaat 
               
               
                 taaccctcactaaagggaacaaaagctgggtaccgggcccacgcgtaatacgactcactatag_1121 
               
               
                 5 
               
               
                   
               
               
                 VZV VEERep.SGPgH-SGPgL (SEQ ID NO: 76) 
               
               
                 1_ 
               
               
                 ataggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcacgttgacatc 
               
               
                 gaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttgaggtagaagccaagca 
               
               
                 ggtcactgataatgaccatgctaatgccagagcgttttcgcatctggcttcaaaactgatcgaaacgg 
               
               
                 aggtggacccatccgacacgatccttgacattggaagtgcgcccgcccgcagaatgtattctaagcac 
               
               
                 aagtatcattgtatctgtccgatgagatgtgcggaagatccggacagattgtataagtatgcaactaa 
               
               
                 gctgaagaaaaactgtaaggaaataactgataaggaattggacaagaaaatgaaggagctcgccgccg 
               
               
                 tcatgagcgaccctgacctggaaactgagactatgtgcctccacgacgacgagtcgtgtcgctacgaa 
               
               
                 gggcaagtcgctgtttaccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaa 
               
               
                 taagggagttagagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctg 
               
               
                 gagcatatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggccta 
               
               
                 tgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatttgaaacc 
               
               
                 atccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggacttactgaggagct 
               
               
                 ggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatgtcggtgtgagactatagtt 
               
               
                 agttgcgacgggtacgtcgttaaaagaatagctatcagtccaggcctgtatgggaagccttcaggcta 
               
               
                 tgctgctacgatgcaccgcgagggattcttgtgctgcaaagtgacagacacattgaacggggagaggg 
               
               
                 tctcttttcccgtgtgcacgtatgtgccagctacattgtgtgaccaaatgactggcatactggcaaca 
               
               
                 gatgtcagtgcggacgacgcgcaaaaactgctggttgggctcaaccagcgtatagtcgtcaacggtcg 
               
               
                 cacccagagaaacaccaataccatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggt 
               
               
                 gggcaaaggaatataaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtc 
               
               
                 atggggtgttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaac 
               
               
                 catcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacattggaga 
               
               
                 tcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctctcattaccgcc 
               
               
                 gaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgtgaagccgaggagttgcg 
               
               
                 cgcagctctaccacctttggcagctgatgttgaggagcccactctggaagccgatgtagacttgatgt 
               
               
                 tacaagaggctggggccggctcagtggagacacctcgtggcttgataaaggttaccagctacgatggc 
               
               
                 gaggacaagatcggctcttacgctgtgctttctccgcaggctgtactcaagagtgaaaaattatcttg 
               
               
                 catccaccctctcgctgaacaagtcatagtgataacacactctggccgaaaagggcgttatgccgtgg 
               
               
                 aaccataccatggtaaagtagtggtgccagagggacatgcaatacccgtccaggactttcaagctctg 
               
               
                 agtgaaagtgccaccattgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccac 
               
               
                 acatggaggagcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcg 
               
               
                 aatacctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctcaca 
               
               
                 ggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgaccagccgctcc 
               
               
                 ttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctggcatcattaaaagcg 
               
               
                 cagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgtgcagaaattataagggacgtc 
               
               
                 aagaaaatgaaagggctggacgtcaatgccagaactgtggactcagtgctcttgaatggatgcaaaca 
               
               
                 ccccgtagagaccctgtatattgacgaagcttttgcttgtcatgcaggtactctcagagcgctcatag 
               
               
                 ccattataagacctaaaaaggcagtgctctgcggggatcccaaacagtgcggtttttttaacatgatg 
               
               
                 tgcctgaaagtgcattttaaccacgagatttgcacacaagtcttccacaaaagcatctctcgccgttg 
               
               
                 cactaaatctgtgacttcggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccga 
               
               
                 aagagactaagattgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcact 
               
               
                 tgtttcagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctgc 
               
               
                 ctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcctctgtacg 
               
               
                 cacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgtgtggaaaacacta 
               
               
                 gccggcgacccatggataaaaacactgactgccaagtaccctgggaatttcactgccacgatagagga 
               
               
                 gtggcaagcagagcatgatgccatcatgaggcacatcttggagagaccggaccctaccgacgtcttcc 
               
               
                 agaataaggcaaacgtgtgttgggccaaggctttagtgccggtgctgaagaccgctggcatagacatg 
               
               
                 accactgaacaatggaacactgtggattattttgaaacggacaaagctcactcagcagagatagtatt 
               
               
                 gaaccaactatgcgtgaggttctttggactcgatctggactccggtctattttctgcacccactgttc 
               
               
                 cgttatccattaggaataatcactgggataactccccgtcgcctaacatgtacgggctgaataaagaa 
               
               
                 gtggtccgtcagctctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatga 
               
               
                 catgaacactggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgc 
               
               
                 ctcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagcaaattg 
               
               
                 aagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggttgactggttgtc 
               
               
                 agaccggcctgaggctaccttcagagctcggctggatttaggcatcccaggtgatgtgcccaaatatg 
               
               
                 acataatatttgttaatgtgaggaccccatataaataccatcactatcagcagtgtgaagaccatgcc 
               
               
                 attaagcttagcatgttgaccaagaaagcttgtctgcatctgaatcccggcggaacctgtgtcagcat 
               
               
                 aggttatggttacgctgacagggccagcgaaagcatcattggtgctatagcgcggcagttcaagtttt 
               
               
                 cccgggtatgcaaaccgaaatcctcacttgaagagacggaagttctgtttgtattcattgggtacgat 
               
               
                 cgcaaggcccgtacgcacaatccttacaagctttcatcaaccttgaccaacatttatacaggttccag 
               
               
                 actccacgaagccggatgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaag 
               
               
                 gagtgattataaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataag 
               
               
                 aaattcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgcagc 
               
               
                 taaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtgacaaacagt 
               
               
                 tggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaagtcagtagcgattcca 
               
               
                 ctgttgtccaccggcatcttttccgggaacaaagatcgactaacccaatcattgaaccatttgctgac 
               
               
                 agctttagacaccactgatgcagatgtagccatatactgcagggacaagaaatgggaaatgactctca 
               
               
                 aggaagcagtggctaggagagaagcagtggaggagatatgcatatccgacgactcttcagtgacagaa 
               
               
                 cctgatgcagagctggtgagggtgcatccgaagagttctttggctggaaggaagggctacagcacaag 
               
               
                 cgatggcaaaactttctcatatttggaagggaccaagtttcaccaggcggccaaggatatagcagaaa 
               
               
                 ttaatgccatgtggcccgttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagc 
               
               
                 atgagcagtattaggtcgaaatgccccgtcgaagagtcggaagcctccacaccacctagcacgctgcc 
               
               
                 ttgcttgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaaa 
               
               
                 ttactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatccaatgctcc 
               
               
                 cagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatctcgtggaaacacc 
               
               
                 accggtagacgagactccggagccatcggcagagaaccaatccacagaggggacacctgaacaaccac 
               
               
                 cacttataaccgaggatgagaccaggactagaacgcctgagccgatcatcatcgaagaggaagaagag 
               
               
                 gatagcataagtttgctgtcagatggcccgacccaccaggtgctgcaagtcgaggcagacattcacgg 
               
               
                 gccgccctctgtatctagctcatcctggtccattcctcatgcatccgactttgatgtggacagtttat 
               
               
                 ccatacttgacaccctggagggagctagcgtgaccagcggggcaacgtcagccgagactaactcttac 
               
               
                 ttcgcaaagagtatggagtttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctcc 
               
               
                 acatcccgctccgcgcacaagaacaccgtcacttgcacccagcagggcctgctcgagaaccagcctag 
               
               
                 tttccaccccgccaggcgtgaatagggtgatcactagagaggagctcgaggcgcttaccccgtcacgc 
               
               
                 actcctagcaggtcggtctcgagaaccagcctggtctccaacccgccaggcgtaaatagggtgattac 
               
               
                 aagagaggagtttgaggcgttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatctttt 
               
               
                 cctccgacaccggtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtg 
               
               
                 ttggagaggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtggagaaca 
               
               
                 tgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggcagaaggaaaagtg 
               
               
                 gagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaaccgtgccttttcaagccc 
               
               
                 caaggtcgcagtggaagcctgtaacgccatgttgaaagagaactttccgactgtggcttcttactgta 
               
               
                 ttattccagagtacgatgcctatttggacatggttgacggagcttcatgctgcttagacactgccagt 
               
               
                 ttttgccctgcaaagctgcgcagctttccaaagaaacactcctatttggaacccacaatacgatcggc 
               
               
                 agtgccttcagcgatccagaacacgctccagaacgtcctggcagctgccacaaaaagaaattgcaatg 
               
               
                 tcacgcaaatgagagaattgcccgtattggattcggcggcctttaatgtggaatgcttcaagaaatat 
               
               
                 gcgtgtaataatgaatattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggt 
               
               
                 aaattacattaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaata 
               
               
                 tgttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactccagga 
               
               
                 acaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctagcaacagcgta 
               
               
                 tctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgcttccgaacattcatacac 
               
               
                 tgtttgatatgtcggctgaagactttgacgctattatagccgagcacttccagcctggggattgtgtt 
               
               
                 ctggaaactgacatcgcgtcgtttgataaaagtgaggacgacgccatggctctgaccgcgttaatgat 
               
               
                 tctggaagacttaggtgtggacgcagagctgttgacgctgattgaggcggctttcggcgaaatttcat 
               
               
                 caatacatttgcccactaaaactaaatttaaattcggagccatgatgaaatctggaatgttcctcaca 
               
               
                 ctgtttgtgaacacagtcattaacattgtaatcgcaagcagagtgttgagagaacggctaaccggatc 
               
               
                 accatgtgcagcattcattggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcag 
               
               
                 acaggtgcgccacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgcct 
               
               
                 tatttctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagaccccct 
               
               
                 aaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacaggagaaggg 
               
               
                 cattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgtgcaaggcagtagaa 
               
               
                 tcaaggtatgaaaccgtaggaacttccatcatagttatggccatgactactctagctagcagtgttaa 
               
               
                 atcattcagctacctgagaggggcccctataactctctacggctaacctgaatggactacgacatagt 
               
               
                 ctagtcgagtctagtcgacgccaccatgttcgccctggtgctggccgtggtcatcctgcctctgtgga 
               
               
                 ccaccgccaacaagagctacgtgacccccacacccgccaccagatccatcggacacatgagcgccctg 
               
               
                 ctgagagagtacagcgaccggaacatgagcctgaagctggaagccttctaccccaccggcttcgacga 
               
               
                 ggaactgatcaagagcctgcactggggcaacgaccggaagcacgtgttcctcgtgatcgtgaaagtga 
               
               
                 accccaccacccacgagggcgacgtcggcctggtcatcttccccaagtacctgctgagcccctaccac 
               
               
                 ttcaaggccgagcacagagcccccttccctgctggccgctttggctttctgagccaccctgtgacccc 
               
               
                 cgacgtgtcattcttcgacagcagcttcgccccctacctgaccacacagcacctggtggccttcacca 
               
               
                 ccttcccccccaatcctctcgtgtggcacctggaaagagccgagacagccgccaccgccgaaagacct 
               
               
                 tttggcgtgtccctgctgcccgccagacctaccgtgcccaagaacaccatcctggaacacaaggccca 
               
               
                 cttcgccacctgggatgccctggccagacacaccttctttagcgccgaggccatcatcaccaacagca 
               
               
                 ccctgagaatccacgtgcccctgttcggcagcgtgtggcccatcagatactgggccacaggcagcgtg 
               
               
                 ctgctgaccagcgatagcggcagagtggaagtgaacatcggcgtgggcttcatgagcagcctgatcag 
               
               
                 cctgagcagcggcctgcccatcgagctgattgtggtgccccacaccgtgaagctgaacgccgtgacca 
               
               
                 gcgacaccacctggttccagctgaacccccctggccctgatcctggccctagttacagagtgtacctg 
               
               
                 ctgggcagaggcctggacatgaacttcagcaagcacgccaccgtggacatctgcgcctaccctgagga 
               
               
                 aagcctggactacagataccacctgagcatggcccacaccgaggccctgagaatgaccaccaaggccg 
               
               
                 accagcacgacatcaacgaggaaagctactaccacattgccgccagaatcgccaccagcatcttcgcc 
               
               
                 ctgagcgagatgggccggaccaccgagtactttctgctggacgagatcgtggacgtgcagtaccagct 
               
               
                 gaagttcctgaactacatcctgatgcggatcggcgctggcgcccaccctaataccatcagcggcacca 
               
               
                 gcgacctgatcttcgccgatcctagccagctgcacgacgagctgagcctgctgttcggccaggtcaaa 
               
               
                 cccgccaacgtggactacttcatcagctacgacgaggcccgggaccagctgaaaacagcctacgccct 
               
               
                 gtccagaggccaggatcatgtgaacgccctgtccctggccaggcgcgtgatcatgagcatctacaagg 
               
               
                 gcctgctggtcaagcagaacctgaacgccaccgagcggcaggccctgttcttcgccagcatgatcctg 
               
               
                 ctgaacttcagagagggcctggaaaacagcagccgggtgctggatggcagaaccaccctgctgctgat 
               
               
                 gaccagcatgtgcacagccgcccatgccacacaggccgccctgaatatccaggaaggcctggcttacc 
               
               
                 tgaaccccagcaagcacatgttcaccatccccaacgtgtacagcccctgcatgggcagcctgagaacc 
               
               
                 gacctgaccgaagagatccacgtgatgaacctgctgtccgccatccccaccagacccggactgaatga 
               
               
                 ggtgctgcacacccagctggacgagtccgagatcttcgacgccgccttcaagaccatgatgatcttta 
               
               
                 ccacctggaccgccaaggacctgcacatcctgcacacacacgtgcccgaggtgttcacatgccaagat 
               
               
                 gccgccgctcggaacggcgagtatgtgctgattctgcctgccgtgcagggccacagctacgtgatcac 
               
               
                 ccggaacaagccccagcggggcctggtgtatagcctggctgacgtggacgtgtacaaccccatcagcg 
               
               
                 tggtgtacctgagcaaggatacctgcgtgtccgagcacggcgtgatcgaaacagtggccctgccccac 
               
               
                 cccgacaacctgaaagagtgcctgtactgcggctccgtgttcctgcggtatctgaccaccggcgccat 
               
               
                 catggacatcatcatcatcgacagcaaggacaccgagagacagctggccgccatgggcaacagcacca 
               
               
                 tcccccccttcaaccccgacatgcacggcgacgatagcaaggccgtgctgctgttccccaacggcacc 
               
               
                 gtggtcacactgctgggcttcgagcggagacaggccatcagaatgagcggccagtacctgggcgcctc 
               
               
                 tctgggtggtgcctttctggccgtcgtgggctttggcatcatcggctggatgctgtgcggcaacagca 
               
               
                 gactgcgcgagtacaacaagatccccctgacctaatctagacgtcgcgaccacccaggatccgcctat 
               
               
                 aactctctacggctaacctgaatggactacgacatagtctagtcgacgccaccatggccagccacaag 
               
               
                 tggctgctgcagatgatcgtgttcctgaaaaccatcacaatcgcctactgcctgcatctgcaggacga 
               
               
                 cacccctctgttcttcggcgccaagcctctgagcgacgtgtccctgatcatcaccgagccttgcgtgt 
               
               
                 ccagcgtgtacgaggcctgggattatgccgcccctcccgtgtccaatctgagcgaagccctgagcggc 
               
               
                 atcgtggtcaagaccaagtgccccgtgcccgaagtgatcctgtggttcaaggacaagcagatggccta 
               
               
                 ctggaccaacccttacgtgaccctgaagggcctgacccagagcgtgggcgaggaacacaagagcggcg 
               
               
                 acatcagagatgccctgctggatgccctgtccggtgtctgggtggacagcacaccctccagcaccaac 
               
               
                 atccccgagaacggctgtgtgtggggagccgaccggctgttccagagagtgtgtcagtaatctagacg 
               
               
                 cggccgcatacagcagcaattggcaagctgcttacatagaactcgcggcgattggcatgccgccttaa 
               
               
                 aatttttattttatttttcttttcttttccgaatcggattttgtttttaatatttcaaaaaaaaaaaa 
               
               
                 aaaaaaaaaaaaaaaaaaaaaaagggtcggcatggcatctccacctcctcgcggtccgacctgggcat 
               
               
                 ccgaaggaggacgcacgtccactcggatggctaagggagagccacgtttaaaccagctccaattcgcc 
               
               
                 ctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctg 
               
               
                 gcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcc 
               
               
                 cgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcgc 
               
               
                 attaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccg 
               
               
                 ctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgg 
               
               
                 gggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtga 
               
               
                 tggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttct 
               
               
                 ttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgattta 
               
               
                 taagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaa 
               
               
                 ttttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctat 
               
               
                 ttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttc 
               
               
                 aataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg 
               
               
                 gcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagtt 
               
               
                 gggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccg 
               
               
                 aagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgac 
               
               
                 gccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagt 
               
               
                 cacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtg 
               
               
                 ataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcac 
               
               
                 aacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacga 
               
               
                 cgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactac 
               
               
                 ttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctg 
               
               
                 cgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcgg 
               
               
                 tatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtc 
               
               
                 aggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa 
               
               
                 ctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggat 
               
               
                 ctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgag 
               
               
                 cgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgc 
               
               
                 ttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttt 
               
               
                 tccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttag 
               
               
                 gccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggct 
               
               
                 gctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgca 
               
               
                 gcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactga 
               
               
                 gatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccg 
               
               
                 gtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatcttta 
               
               
                 tagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgga 
               
               
                 gcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcac 
               
               
                 atgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgatac 
               
               
                 cgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatac 
               
               
                 gcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgg 
               
               
                 aaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttaca 
               
               
                 ctttatgctcccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagct 
               
               
                 atgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctgggtaccgggccc 
               
               
                 acgcgtaatacgactcactatag_13827 
               
               
                   
               
               
                 VZV VEERep.SGPgE (SEQ ID NO: 77): 
               
               
                 1 
               
               
                 ataggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcacgttgacatc 
               
               
                 gaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttgaggtagaagccaagca 
               
               
                 ggtcactgataatgaccatgctaatgccagagcgttttcgcatctggcttcaaaactgatcgaaacgg 
               
               
                 aggtggacccatccgacacgatccttgacattggaagtgcgcccgcccgcagaatgtattctaagcac 
               
               
                 aagtatcattgtatctgtccgatgagatgtgcggaagatccggacagattgtataagtatgcaactaa 
               
               
                 gctgaagaaaaactgtaaggaaataactgataaggaattggacaagaaaatgaaggagctcgccgccg 
               
               
                 tcatgagcgaccctgacctggaaactgagactatgtgcctccacgacgacgagtcgtgtcgctacgaa 
               
               
                 gggcaagtcgctgtttaccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaa 
               
               
                 taagggagttagagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctg 
               
               
                 gagcatatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggccta 
               
               
                 tgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatttgaaacc 
               
               
                 atccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggacttactgaggagct 
               
               
                 ggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatgtcggtgtgagactatagtt 
               
               
                 agttgcgacgggtacgtcgttaaaagaatagctatcagtccaggcctgtatgggaagccttcaggcta 
               
               
                 tgctgctacgatgcaccgcgagggattcttgtgctgcaaagtgacagacacattgaacggggagaggg 
               
               
                 tctcttttcccgtgtgcacgtatgtgccagctacattgtgtgaccaaatgactggcatactggcaaca 
               
               
                 gatgtcagtgcggacgacgcgcaaaaactgctggttgggctcaaccagcgtatagtcgtcaacggtcg 
               
               
                 cacccagagaaacaccaataccatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggt 
               
               
                 gggcaaaggaatataaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtc 
               
               
                 atggggtgttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaac 
               
               
                 catcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacattggaga 
               
               
                 tcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctctcattaccgcc 
               
               
                 gaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgtgaagccgaggagttgcg 
               
               
                 cgcagctctaccacctttggcagctgatgttgaggagcccactctggaagccgatgtagacttgatgt 
               
               
                 tacaagaggctggggccggctcagtggagacacctcgtggcttgataaaggttaccagctacgatggc 
               
               
                 gaggacaagatcggctcttacgctgtgctttctccgcaggctgtactcaagagtgaaaaattatcttg 
               
               
                 catccaccctctcgctgaacaagtcatagtgataacacactctggccgaaaagggcgttatgccgtgg 
               
               
                 aaccataccatggtaaagtagtggtgccagagggacatgcaatacccgtccaggactttcaagctctg 
               
               
                 agtgaaagtgccaccattgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccac 
               
               
                 acatggaggagcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcg 
               
               
                 aatacctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctcaca 
               
               
                 ggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgaccagccgctcc 
               
               
                 ttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctggcatcattaaaagcg 
               
               
                 cagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgtgcagaaattataagggacgtc 
               
               
                 aagaaaatgaaagggctggacgtcaatgccagaactgtggactcagtgctcttgaatggatgcaaaca 
               
               
                 ccccgtagagaccctgtatattgacgaagcttttgcttgtcatgcaggtactctcagagcgctcatag 
               
               
                 ccattataagacctaaaaaggcagtgctctgcggggatcccaaacagtgcggtttttttaacatgatg 
               
               
                 tgcctgaaagtgcattttaaccacgagatttgcacacaagtcttccacaaaagcatctctcgccgttg 
               
               
                 cactaaatctgtgacttcggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccga 
               
               
                 aagagactaagattgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcact 
               
               
                 tgtttcagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctgc 
               
               
                 ctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcctctgtacg 
               
               
                 cacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgtgtggaaaacacta 
               
               
                 gccggcgacccatggataaaaacactgactgccaagtaccctgggaatttcactgccacgatagagga 
               
               
                 gtggcaagcagagcatgatgccatcatgaggcacatcttggagagaccggaccctaccgacgtcttcc 
               
               
                 agaataaggcaaacgtgtgttgggccaaggctttagtgccggtgctgaagaccgctggcatagacatg 
               
               
                 accactgaacaatggaacactgtggattattttgaaacggacaaagctcactcagcagagatagtatt 
               
               
                 gaaccaactatgcgtgaggttctttggactcgatctggactccggtctattttctgcacccactgttc 
               
               
                 cgttatccattaggaataatcactgggataactccccgtcgcctaacatgtacgggctgaataaagaa 
               
               
                 gtggtccgtcagctctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatga 
               
               
                 catgaacactggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgc 
               
               
                 ctcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagcaaattg 
               
               
                 aagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggttgactggttgtc 
               
               
                 agaccggcctgaggctaccttcagagctcggctggatttaggcatcccaggtgatgtgcccaaatatg 
               
               
                 acataatatttgttaatgtgaggaccccatataaataccatcactatcagcagtgtgaagaccatgcc 
               
               
                 attaagcttagcatgttgaccaagaaagcttgtctgcatctgaatcccggcggaacctgtgtcagcat 
               
               
                 aggttatggttacgctgacagggccagcgaaagcatcattggtgctatagcgcggcagttcaagtttt 
               
               
                 cccgggtatgcaaaccgaaatcctcacttgaagagacggaagttctgtttgtattcattgggtacgat 
               
               
                 cgcaaggcccgtacgcacaatccttacaagctttcatcaaccttgaccaacatttatacaggttccag 
               
               
                 actccacgaagccggatgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaag 
               
               
                 gagtgattataaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataag 
               
               
                 aaattcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgcagc 
               
               
                 taaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtgacaaacagt 
               
               
                 tggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaagtcagtagcgattcca 
               
               
                 ctgttgtccaccggcatcttttccgggaacaaagatcgactaacccaatcattgaaccatttgctgac 
               
               
                 agctttagacaccactgatgcagatgtagccatatactgcagggacaagaaatgggaaatgactctca 
               
               
                 aggaagcagtggctaggagagaagcagtggaggagatatgcatatccgacgactcttcagtgacagaa 
               
               
                 cctgatgcagagctggtgagggtgcatccgaagagttctttggctggaaggaagggctacagcacaag 
               
               
                 cgatggcaaaactttctcatatttggaagggaccaagtttcaccaggcggccaaggatatagcagaaa 
               
               
                 ttaatgccatgtggcccgttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagc 
               
               
                 atgagcagtattaggtcgaaatgccccgtcgaagagtcggaagcctccacaccacctagcacgctgcc 
               
               
                 ttgcttgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaaa 
               
               
                 ttactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatccaatgctcc 
               
               
                 cagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatctcgtggaaacacc 
               
               
                 accggtagacgagactccggagccatcggcagagaaccaatccacagaggggacacctgaacaaccac 
               
               
                 cacttataaccgaggatgagaccaggactagaacgcctgagccgatcatcatcgaagaggaagaagag 
               
               
                 gatagcataagtttgctgtcagatggcccgacccaccaggtgctgcaagtcgaggcagacattcacgg 
               
               
                 gccgccctctgtatctagctcatcctggtccattcctcatgcatccgactttgatgtggacagtttat 
               
               
                 ccatacttgacaccctggagggagctagcgtgaccagcggggcaacgtcagccgagactaactcttac 
               
               
                 ttcgcaaagagtatggagtttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctcc 
               
               
                 acatcccgctccgcgcacaagaacaccgtcacttgcacccagcagggcctgctcgagaaccagcctag 
               
               
                 tttccaccccgccaggcgtgaatagggtgatcactagagaggagctcgaggcgcttaccccgtcacgc 
               
               
                 actcctagcaggtcggtctcgagaaccagcctggtctccaacccgccaggcgtaaatagggtgattac 
               
               
                 aagagaggagtttgaggcgttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatctttt 
               
               
                 cctccgacaccggtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtg 
               
               
                 ttggagaggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtggagaaca 
               
               
                 tgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggcagaaggaaaagtg 
               
               
                 gagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaaccgtgccttttcaagccc 
               
               
                 caaggtcgcagtggaagcctgtaacgccatgttgaaagagaactttccgactgtggcttcttactgta 
               
               
                 ttattccagagtacgatgcctatttggacatggttgacggagcttcatgctgcttagacactgccagt 
               
               
                 ttttgccctgcaaagctgcgcagctttccaaagaaacactcctatttggaacccacaatacgatcggc 
               
               
                 agtgccttcagcgatccagaacacgctccagaacgtcctggcagctgccacaaaaagaaattgcaatg 
               
               
                 tcacgcaaatgagagaattgcccgtattggattcggcggcctttaatgtggaatgcttcaagaaatat 
               
               
                 gcgtgtaataatgaatattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggt 
               
               
                 aaattacattaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaata 
               
               
                 tgttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactccagga 
               
               
                 acaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctagcaacagcgta 
               
               
                 tctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgcttccgaacattcatacac 
               
               
                 tgtttgatatgtcggctgaagactttgacgctattatagccgagcacttccagcctggggattgtgtt 
               
               
                 ctggaaactgacatcgcgtcgtttgataaaagtgaggacgacgccatggctctgaccgcgttaatgat 
               
               
                 tctggaagacttaggtgtggacgcagagctgttgacgctgattgaggcggctttcggcgaaatttcat 
               
               
                 caatacatttgcccactaaaactaaatttaaattcggagccatgatgaaatctggaatgttcctcaca 
               
               
                 ctgtttgtgaacacagtcattaacattgtaatcgcaagcagagtgttgagagaacggctaaccggatc 
               
               
                 accatgtgcagcattcattggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcag 
               
               
                 acaggtgcgccacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgcct 
               
               
                 tatttctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagaccccct 
               
               
                 aaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacaggagaaggg 
               
               
                 cattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgtgcaaggcagtagaa 
               
               
                 tcaaggtatgaaaccgtaggaacttccatcatagttatggccatgactactctagctagcagtgttaa 
               
               
                 atcattcagctacctgagaggggcccctataactctctacggctaacctgaatggactacgacatagt 
               
               
                 ctagtcgagtctagtcgacgccaccatgggcaccgtgaacaagcctgtcgtgggcgtgctgatgggct 
               
               
                 tcggcatcatcaccggcaccctgagaatcaccaaccctgtgcgggccagcgtgctgagatacgacgac 
               
               
                 ttccacatcgacgaggacaagctggacaccaacagcgtgtacgagccctactaccacagcgaccacgc 
               
               
                 cgagagcagctgggtcaacagaggcgagagcagccggaaggcctacgaccacaacagcccctacatct 
               
               
                 ggccccggaacgactacgacggcttcctggaaaacgcccacgagcaccacggcgtgtacaatcagggc 
               
               
                 agaggcatcgacagcggcgagagactgatgcagcccacacagatgagcgcccaggaagatctgggcga 
               
               
                 cgacacaggcatccacgtgatccccaccctgaacggcgacgaccggcacaagatcgtgaacgtggacc 
               
               
                 agcggcagtacggcgacgtgttcaagggcgacctgaaccctaagccccagggccagagactgatcgag 
               
               
                 gtgtccgtggaagagaaccaccccttcaccctgagagcccccatccagagaatctacggcgtgcggta 
               
               
                 taccgagacttggagcttcctgcccagcctgacctgtacaggcgacgccgctcctgccatccagcaca 
               
               
                 tctgcctgaagcacaccacctgtttccaggacgtggtggtggacgtggactgcgccgagaacaccaaa 
               
               
                 gaggaccagctggccgagatcagctaccggttccagggcaagaaagaggccgaccagccctggatcgt 
               
               
                 ggtcaataccagcaccctgttcgacgagctggaactggacccccccgagattgaacccggcgtgctga 
               
               
                 aggtgctgcggaccgagaagcagtacctgggcgtgtacatctggaacatgcggggctccgacggcacc 
               
               
                 tctacctacgccaccttcctggtcacatggaagggcgacgagaaaacccggaaccctacccctgccgt 
               
               
                 gacccctcagcctagaggcgccgagttccatatgtggaattaccactcccacgtgttcagcgtgggcg 
               
               
                 acaccttcagcctggccatgcatctgcagtacaagatccacgaggcccccttcgacctgctgctggaa 
               
               
                 tggctgtacgtgcccatcgaccctacctgccagcccatgcggctgtacagcacctgtctgtaccaccc 
               
               
                 caacgcccctcagtgcctgagccacatgaacagcggctgcaccttcaccagccctcacctggctcaga 
               
               
                 gggtggccagcaccgtgtaccagaattgcgagcacgccgacaactacaccgcctactgcctgggcatc 
               
               
                 agccacatggaacccagcttcggcctgatcctgcacgatggcggcaccaccctgaagttcgtggacac 
               
               
                 acccgagagcctgagcggcctgtacgtgttcgtggtgtacttcaacggccacgtggaagccgtggcct 
               
               
                 acaccgtggtgtccaccgtggaccacttcgtgaacgccatcgaggaaagaggcttcccacccacagcc 
               
               
                 ggacagcctccagccaccaccaagcccaaagaaatcacccccgtgaaccccggcaccagccccctgct 
               
               
                 gagatatgctgcttggacaggcggactggccgctgtggtgctgctgtgcctggtcatcttcctgatct 
               
               
                 gcaccgccaagcggatgagagtgaaggcctaccgggtggacaagtccccctacaaccagagcatgtac 
               
               
                 tacgccggcctgcccgtggacgatttcgaggatagcgagagcaccgacaccgaggaagagttcggcaa 
               
               
                 cgccatcggcggatctcacggcggcagcagctacaccgtgtacatcgacaagaccagataatctagac 
               
               
                 gcggccgcatacagcagcaattggcaagctgcttacatagaactcgcggcgattggcatgccgcctta 
               
               
                 aaatttttattttatttttcttttcttttccgaatcggattttgtttttaatatttcaaaaaaaaaaa 
               
               
                 aaaaaaaaaaaaaaaaaaaaaaaagggtcggcatggcatctccacctcctcgcggtccgacctgggca 
               
               
                 tccgaaggaggacgcacgtccactcggatggctaagggagagccacgtttaaaccagctccaattcgc 
               
               
                 cctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccct 
               
               
                 ggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggc 
               
               
                 ccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcg 
               
               
                 cattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgccc 
               
               
                 gctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcg 
               
               
                 ggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtg 
               
               
                 atggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttc 
               
               
                 tttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgattt 
               
               
                 ataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcga 
               
               
                 attttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaaccccta 
               
               
                 tttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgctt 
               
               
                 caataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgc 
               
               
                 ggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagt 
               
               
                 tgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgcccc 
               
               
                 gaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattga 
               
               
                 cgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccag 
               
               
                 tcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagt 
               
               
                 gataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgca 
               
               
                 caacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacg 
               
               
                 acgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaacta 
               
               
                 cttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttct 
               
               
                 gcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcg 
               
               
                 gtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagt 
               
               
                 caggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggta 
               
               
                 actgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaagga 
               
               
                 tctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactga 
               
               
                 gcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctg 
               
               
                 cttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactcttt 
               
               
                 ttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagtta 
               
               
                 ggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggc 
               
               
                 tgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgc 
               
               
                 agcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactg 
               
               
                 agatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatcc 
               
               
                 ggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatcttt 
               
               
                 atagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgg 
               
               
                 agcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctca 
               
               
                 catgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgata 
               
               
                 ccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaata 
               
               
                 cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactg 
               
               
                 gaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttac 
               
               
                 actttatgctcccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagc 
               
               
                 tatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctgggtaccgggcc 
               
               
                 cacgcgtaatacgactcactatag_12604 
               
               
                   
               
               
                 VZV VEERep.SGPgI (SEQ ID NO: 78) 
               
               
                 1 
               
               
                 ataggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcacgttgacatc 
               
               
                 gaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttgaggtagaagccaagca 
               
               
                 ggtcactgataatgaccatgctaatgccagagcgttttcgcatctggcttcaaaactgatcgaaacgg 
               
               
                 aggtggacccatccgacacgatccttgacattggaagtgcgcccgcccgcagaatgtattctaagcac 
               
               
                 aagtatcattgtatctgtccgatgagatgtgcggaagatccggacagattgtataagtatgcaactaa 
               
               
                 gctgaagaaaaactgtaaggaaataactgataaggaattggacaagaaaatgaaggagctcgccgccg 
               
               
                 tcatgagcgaccctgacctggaaactgagactatgtgcctccacgacgacgagtcgtgtcgctacgaa 
               
               
                 gggcaagtcgctgtttaccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaa 
               
               
                 taagggagttagagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctg 
               
               
                 gagcatatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggccta 
               
               
                 tgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatttgaaacc 
               
               
                 atccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggacttactgaggagct 
               
               
                 ggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatgtcggtgtgagactatagtt 
               
               
                 agttgcgacgggtacgtcgttaaaagaatagctatcagtccaggcctgtatgggaagccttcaggcta 
               
               
                 tgctgctacgatgcaccgcgagggattcttgtgctgcaaagtgacagacacattgaacggggagaggg 
               
               
                 tctcttttcccgtgtgcacgtatgtgccagctacattgtgtgaccaaatgactggcatactggcaaca 
               
               
                 gatgtcagtgcggacgacgcgcaaaaactgctggttgggctcaaccagcgtatagtcgtcaacggtcg 
               
               
                 cacccagagaaacaccaataccatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggt 
               
               
                 gggcaaaggaatataaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtc 
               
               
                 atggggtgttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaac 
               
               
                 catcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacattggaga 
               
               
                 tcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctctcattaccgcc 
               
               
                 gaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgtgaagccgaggagttgcg 
               
               
                 cgcagctctaccacctttggcagctgatgttgaggagcccactctggaagccgatgtagacttgatgt 
               
               
                 tacaagaggctggggccggctcagtggagacacctcgtggcttgataaaggttaccagctacgatggc 
               
               
                 gaggacaagatcggctcttacgctgtgctttctccgcaggctgtactcaagagtgaaaaattatcttg 
               
               
                 catccaccctctcgctgaacaagtcatagtgataacacactctggccgaaaagggcgttatgccgtgg 
               
               
                 aaccataccatggtaaagtagtggtgccagagggacatgcaatacccgtccaggactttcaagctctg 
               
               
                 agtgaaagtgccaccattgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccac 
               
               
                 acatggaggagcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcg 
               
               
                 aatacctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctcaca 
               
               
                 ggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgaccagccgctcc 
               
               
                 ttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctggcatcattaaaagcg 
               
               
                 cagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgtgcagaaattataagggacgtc 
               
               
                 aagaaaatgaaagggctggacgtcaatgccagaactgtggactcagtgctcttgaatggatgcaaaca 
               
               
                 ccccgtagagaccctgtatattgacgaagcttttgcttgtcatgcaggtactctcagagcgctcatag 
               
               
                 ccattataagacctaaaaaggcagtgctctgcggggatcccaaacagtgcggtttttttaacatgatg 
               
               
                 tgcctgaaagtgcattttaaccacgagatttgcacacaagtcttccacaaaagcatctctcgccgttg 
               
               
                 cactaaatctgtgacttcggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccga 
               
               
                 aagagactaagattgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcact 
               
               
                 tgtttcagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctgc 
               
               
                 ctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcctctgtacg 
               
               
                 cacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgtgtggaaaacacta 
               
               
                 gccggcgacccatggataaaaacactgactgccaagtaccctgggaatttcactgccacgatagagga 
               
               
                 gtggcaagcagagcatgatgccatcatgaggcacatcttggagagaccggaccctaccgacgtcttcc 
               
               
                 agaataaggcaaacgtgtgttgggccaaggctttagtgccggtgctgaagaccgctggcatagacatg 
               
               
                 accactgaacaatggaacactgtggattattttgaaacggacaaagctcactcagcagagatagtatt 
               
               
                 gaaccaactatgcgtgaggttctttggactcgatctggactccggtctattttctgcacccactgttc 
               
               
                 cgttatccattaggaataatcactgggataactccccgtcgcctaacatgtacgggctgaataaagaa 
               
               
                 gtggtccgtcagctctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatga 
               
               
                 catgaacactggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgc 
               
               
                 ctcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagcaaattg 
               
               
                 aagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggttgactggttgtc 
               
               
                 agaccggcctgaggctaccttcagagctcggctggatttaggcatcccaggtgatgtgcccaaatatg 
               
               
                 acataatatttgttaatgtgaggaccccatataaataccatcactatcagcagtgtgaagaccatgcc 
               
               
                 attaagcttagcatgttgaccaagaaagcttgtctgcatctgaatcccggcggaacctgtgtcagcat 
               
               
                 aggttatggttacgctgacagggccagcgaaagcatcattggtgctatagcgcggcagttcaagtttt 
               
               
                 cccgggtatgcaaaccgaaatcctcacttgaagagacggaagttctgtttgtattcattgggtacgat 
               
               
                 cgcaaggcccgtacgcacaatccttacaagctttcatcaaccttgaccaacatttatacaggttccag 
               
               
                 actccacgaagccggatgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaag 
               
               
                 gagtgattataaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataag 
               
               
                 aaattcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgcagc 
               
               
                 taaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtgacaaacagt 
               
               
                 tggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaagtcagtagcgattcca 
               
               
                 ctgttgtccaccggcatcttttccgggaacaaagatcgactaacccaatcattgaaccatttgctgac 
               
               
                 agctttagacaccactgatgcagatgtagccatatactgcagggacaagaaatgggaaatgactctca 
               
               
                 aggaagcagtggctaggagagaagcagtggaggagatatgcatatccgacgactcttcagtgacagaa 
               
               
                 cctgatgcagagctggtgagggtgcatccgaagagttctttggctggaaggaagggctacagcacaag 
               
               
                 cgatggcaaaactttctcatatttggaagggaccaagtttcaccaggcggccaaggatatagcagaaa 
               
               
                 ttaatgccatgtggcccgttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagc 
               
               
                 atgagcagtattaggtcgaaatgccccgtcgaagagtcggaagcctccacaccacctagcacgctgcc 
               
               
                 ttgcttgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaaa 
               
               
                 ttactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatccaatgctcc 
               
               
                 cagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatctcgtggaaacacc 
               
               
                 accggtagacgagactccggagccatcggcagagaaccaatccacagaggggacacctgaacaaccac 
               
               
                 cacttataaccgaggatgagaccaggactagaacgcctgagccgatcatcatcgaagaggaagaagag 
               
               
                 gatagcataagtttgctgtcagatggcccgacccaccaggtgctgcaagtcgaggcagacattcacgg 
               
               
                 gccgccctctgtatctagctcatcctggtccattcctcatgcatccgactttgatgtggacagtttat 
               
               
                 ccatacttgacaccctggagggagctagcgtgaccagcggggcaacgtcagccgagactaactcttac 
               
               
                 ttcgcaaagagtatggagtttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctcc 
               
               
                 acatcccgctccgcgcacaagaacaccgtcacttgcacccagcagggcctgctcgagaaccagcctag 
               
               
                 tttccaccccgccaggcgtgaatagggtgatcactagagaggagctcgaggcgcttaccccgtcacgc 
               
               
                 actcctagcaggtcggtctcgagaaccagcctggtctccaacccgccaggcgtaaatagggtgattac 
               
               
                 aagagaggagtttgaggcgttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatctttt 
               
               
                 cctccgacaccggtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtg 
               
               
                 ttggagaggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtggagaaca 
               
               
                 tgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggcagaaggaaaagtg 
               
               
                 gagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaaccgtgccttttcaagccc 
               
               
                 caaggtcgcagtggaagcctgtaacgccatgttgaaagagaactttccgactgtggcttcttactgta 
               
               
                 ttattccagagtacgatgcctatttggacatggttgacggagcttcatgctgcttagacactgccagt 
               
               
                 ttttgccctgcaaagctgcgcagctttccaaagaaacactcctatttggaacccacaatacgatcggc 
               
               
                 agtgccttcagcgatccagaacacgctccagaacgtcctggcagctgccacaaaaagaaattgcaatg 
               
               
                 tcacgcaaatgagagaattgcccgtattggattcggcggcctttaatgtggaatgcttcaagaaatat 
               
               
                 gcgtgtaataatgaatattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggt 
               
               
                 aaattacattaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaata 
               
               
                 tgttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactccagga 
               
               
                 acaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctagcaacagcgta 
               
               
                 tctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgcttccgaacattcatacac 
               
               
                 tgtttgatatgtcggctgaagactttgacgctattatagccgagcacttccagcctggggattgtgtt 
               
               
                 ctggaaactgacatcgcgtcgtttgataaaagtgaggacgacgccatggctctgaccgcgttaatgat 
               
               
                 tctggaagacttaggtgtggacgcagagctgttgacgctgattgaggcggctttcggcgaaatttcat 
               
               
                 caatacatttgcccactaaaactaaatttaaattcggagccatgatgaaatctggaatgttcctcaca 
               
               
                 ctgtttgtgaacacagtcattaacattgtaatcgcaagcagagtgttgagagaacggctaaccggatc 
               
               
                 accatgtgcagcattcattggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcag 
               
               
                 acaggtgcgccacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgcct 
               
               
                 tatttctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagaccccct 
               
               
                 aaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacaggagaaggg 
               
               
                 cattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgtgcaaggcagtagaa 
               
               
                 tcaaggtatgaaaccgtaggaacttccatcatagttatggccatgactactctagctagcagtgttaa 
               
               
                 atcattcagctacctgagaggggcccctataactctctacggctaacctgaatggactacgacatagt 
               
               
                 ctagtcgagtctagtcgacgccaccatgtttctgatccagtgcctgatcagcgccgtgatcttctata 
               
               
                 ttcaagtcacaaacgccctgatctttaagggcgaccacgtgtcactgcaggtcaacagcagcctgacc 
               
               
                 agcatcctgatccccatgcagaacgacaattacaccgagatcaagggccagctggtgttcatcggcga 
               
               
                 gcagctgcccaccggcaccaattacagcggcaccctggaactgctgtacgccgataccgtggccttct 
               
               
                 gcttcagaagcgtgcaggtcatcagatacgacggctgcccccggatcagaaccagcgccttcatcagc 
               
               
                 tgccggtacaagcacagctggcactacggcaacagcaccgaccggatcagcaccgaacctgatgccgg 
               
               
                 cgtgatgctgaagatcaccaagcccggcatcaacgacgccggcgtgtacgtgctgctcgtgcggctgg 
               
               
                 atcacagcagaagcaccgacggcttcatcctgggcgtgaacgtgtacaccgccggcagccaccacaac 
               
               
                 atccacggcgtgatctacaccagccccagcctgcagaacggctacagcaccagagccctgttccagca 
               
               
                 ggccagactgtgcgatctgcccgccacacctaagggcagcggcacaagcctgtttcagcacatgctgg 
               
               
                 acctgagagccggcaagagcctggaagataacccctggctgcacgaggacgtggtcaccaccgagaca 
               
               
                 aagagcgtggtcaaagagggcatcgagaaccacgtgtaccccaccgacatgagcaccctgcccgagaa 
               
               
                 gtccctgaacgacccccctgagaacctgctgatcatcatccccatcgtggccagcgtgatgatcctga 
               
               
                 ccgccatggtcatcgtgatcgtgatcagcgtgaagcggcggagaatcaagaagcaccccatctaccgg 
               
               
                 cccaacaccaagaccagacggggcatccagaacgccacccctgagtccgacgtgatgctggaagccgc 
               
               
                 cattgcccagctggccaccatcagagaggaaagcccccctcacagcgtcgtgaaccccttcgtgaagt 
               
               
                 aatctagacgcggccgcatacagcagcaattggcaagctgcttacatagaactcgcggcgattggcat 
               
               
                 gccgccttaaaatttttattttatttttcttttcttttccgaatcggattttgtttttaatatttcaa 
               
               
                 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaagggtcggcatggcatctccacctcctcgcggtccg 
               
               
                 acctgggcatccgaaggaggacgcacgtccactcggatggctaagggagagccacgtttaaaccagct 
               
               
                 ccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgg 
               
               
                 gaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatag 
               
               
                 cgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccct 
               
               
                 gtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgcc 
               
               
                 ctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagc 
               
               
                 tctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttg 
               
               
                 attagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggag 
               
               
                 tccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattc 
               
               
                 ttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaat 
               
               
                 ttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcg 
               
               
                 gaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctga 
               
               
                 taaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcc 
               
               
                 cttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctg 
               
               
                 aagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagt 
               
               
                 tttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatc 
               
               
                 ccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagt 
               
               
                 actcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccata 
               
               
                 accatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgc 
               
               
                 ttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcca 
               
               
                 taccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaact 
               
               
                 ggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcagg 
               
               
                 accacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtg 
               
               
                 ggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacg 
               
               
                 acggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaa 
               
               
                 gcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaat 
               
               
                 ttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcg 
               
               
                 ttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgt 
               
               
                 aatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctac 
               
               
                 caactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtag 
               
               
                 ccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgtt 
               
               
                 accagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccgg 
               
               
                 ataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctac 
               
               
                 accgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcgga 
               
               
                 caggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcct 
               
               
                 ggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtca 
               
               
                 ggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggcc 
               
               
                 ttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagt 
               
               
                 gagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagag 
               
               
                 cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtt 
               
               
                 tcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccc 
               
               
                 aggctttacactttatgctcccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 
               
               
                 ggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggg 
               
               
                 taccgggcccacgcgtaatacgactcactatag_11797 
               
               
                   
               
               
                 VZV VEErep.SGPgE-SGPgI (SEQ ID NO: 79): 
               
               
                 1_ 
               
               
                 ataggcggcgcatgagagaagcccagaccaattacctacccaaaatggagaaagttcacgttgacatc 
               
               
                 gaggaagacagcccattcctcagagctttgcagcggagcttcccgcagtttgaggtagaagccaagca 
               
               
                 ggtcactgataatgaccatgctaatgccagagcgttttcgcatctggcttcaaaactgatcgaaacgg 
               
               
                 aggtggacccatccgacacgatccttgacattggaagtgcgcccgcccgcagaatgtattctaagcac 
               
               
                 aagtatcattgtatctgtccgatgagatgtgcggaagatccggacagattgtataagtatgcaactaa 
               
               
                 gctgaagaaaaactgtaaggaaataactgataaggaattggacaagaaaatgaaggagctcgccgccg 
               
               
                 tcatgagcgaccctgacctggaaactgagactatgtgcctccacgacgacgagtcgtgtcgctacgaa 
               
               
                 gggcaagtcgctgtttaccaggatgtatacgcggttgacggaccgacaagtctctatcaccaagccaa 
               
               
                 taagggagttagagtcgcctactggataggctttgacaccaccccttttatgtttaagaacttggctg 
               
               
                 gagcatatccatcatactctaccaactgggccgacgaaaccgtgttaacggctcgtaacataggccta 
               
               
                 tgcagctctgacgttatggagcggtcacgtagagggatgtccattcttagaaagaagtatttgaaacc 
               
               
                 atccaacaatgttctattctctgttggctcgaccatctaccacgagaagagggacttactgaggagct 
               
               
                 ggcacctgccgtctgtatttcacttacgtggcaagcaaaattacacatgtcggtgtgagactatagtt 
               
               
                 agttgcgacgggtacgtcgttaaaagaatagctatcagtccaggcctgtatgggaagccttcaggcta 
               
               
                 tgctgctacgatgcaccgcgagggattcttgtgctgcaaagtgacagacacattgaacggggagaggg 
               
               
                 tctcttttcccgtgtgcacgtatgtgccagctacattgtgtgaccaaatgactggcatactggcaaca 
               
               
                 gatgtcagtgcggacgacgcgcaaaaactgctggttgggctcaaccagcgtatagtcgtcaacggtcg 
               
               
                 cacccagagaaacaccaataccatgaaaaattaccttttgcccgtagtggcccaggcatttgctaggt 
               
               
                 gggcaaaggaatataaggaagatcaagaagatgaaaggccactaggactacgagatagacagttagtc 
               
               
                 atggggtgttgttgggcttttagaaggcacaagataacatctatttataagcgcccggatacccaaac 
               
               
                 catcatcaaagtgaacagcgatttccactcattcgtgctgcccaggataggcagtaacacattggaga 
               
               
                 tcgggctgagaacaagaatcaggaaaatgttagaggagcacaaggagccgtcacctctcattaccgcc 
               
               
                 gaggacgtacaagaagctaagtgcgcagccgatgaggctaaggaggtgcgtgaagccgaggagttgcg 
               
               
                 cgcagctctaccacctttggcagctgatgttgaggagcccactctggaagccgatgtagacttgatgt 
               
               
                 tacaagaggctggggccggctcagtggagacacctcgtggcttgataaaggttaccagctacgatggc 
               
               
                 gaggacaagatcggctcttacgctgtgctttctccgcaggctgtactcaagagtgaaaaattatcttg 
               
               
                 catccaccctctcgctgaacaagtcatagtgataacacactctggccgaaaagggcgttatgccgtgg 
               
               
                 aaccataccatggtaaagtagtggtgccagagggacatgcaatacccgtccaggactttcaagctctg 
               
               
                 agtgaaagtgccaccattgtgtacaacgaacgtgagttcgtaaacaggtacctgcaccatattgccac 
               
               
                 acatggaggagcgctgaacactgatgaagaatattacaaaactgtcaagcccagcgagcacgacggcg 
               
               
                 aatacctgtacgacatcgacaggaaacagtgcgtcaagaaagaactagtcactgggctagggctcaca 
               
               
                 ggcgagctggtggatcctcccttccatgaattcgcctacgagagtctgagaacacgaccagccgctcc 
               
               
                 ttaccaagtaccaaccataggggtgtatggcgtgccaggatcaggcaagtctggcatcattaaaagcg 
               
               
                 cagtcaccaaaaaagatctagtggtgagcgccaagaaagaaaactgtgcagaaattataagggacgtc 
               
               
                 aagaaaatgaaagggctggacgtcaatgccagaactgtggactcagtgctcttgaatggatgcaaaca 
               
               
                 ccccgtagagaccctgtatattgacgaagcttttgcttgtcatgcaggtactctcagagcgctcatag 
               
               
                 ccattataagacctaaaaaggcagtgctctgcggggatcccaaacagtgcggtttttttaacatgatg 
               
               
                 tgcctgaaagtgcattttaaccacgagatttgcacacaagtcttccacaaaagcatctctcgccgttg 
               
               
                 cactaaatctgtgacttcggtcgtctcaaccttgttttacgacaaaaaaatgagaacgacgaatccga 
               
               
                 aagagactaagattgtgattgacactaccggcagtaccaaacctaagcaggacgatctcattctcact 
               
               
                 tgtttcagagggtgggtgaagcagttgcaaatagattacaaaggcaacgaaataatgacggcagctgc 
               
               
                 ctctcaagggctgacccgtaaaggtgtgtatgccgttcggtacaaggtgaatgaaaatcctctgtacg 
               
               
                 cacccacctcagaacatgtgaacgtcctactgacccgcacggaggaccgcatcgtgtggaaaacacta 
               
               
                 gccggcgacccatggataaaaacactgactgccaagtaccctgggaatttcactgccacgatagagga 
               
               
                 gtggcaagcagagcatgatgccatcatgaggcacatcttggagagaccggaccctaccgacgtcttcc 
               
               
                 agaataaggcaaacgtgtgttgggccaaggctttagtgccggtgctgaagaccgctggcatagacatg 
               
               
                 accactgaacaatggaacactgtggattattttgaaacggacaaagctcactcagcagagatagtatt 
               
               
                 gaaccaactatgcgtgaggttctttggactcgatctggactccggtctattttctgcacccactgttc 
               
               
                 cgttatccattaggaataatcactgggataactccccgtcgcctaacatgtacgggctgaataaagaa 
               
               
                 gtggtccgtcagctctctcgcaggtacccacaactgcctcgggcagttgccactggaagagtctatga 
               
               
                 catgaacactggtacactgcgcaattatgatccgcgcataaacctagtacctgtaaacagaagactgc 
               
               
                 ctcatgctttagtcctccaccataatgaacacccacagagtgacttttcttcattcgtcagcaaattg 
               
               
                 aagggcagaactgtcctggtggtcggggaaaagttgtccgtcccaggcaaaatggttgactggttgtc 
               
               
                 agaccggcctgaggctaccttcagagctcggctggatttaggcatcccaggtgatgtgcccaaatatg 
               
               
                 acataatatttgttaatgtgaggaccccatataaataccatcactatcagcagtgtgaagaccatgcc 
               
               
                 attaagcttagcatgttgaccaagaaagcttgtctgcatctgaatcccggcggaacctgtgtcagcat 
               
               
                 aggttatggttacgctgacagggccagcgaaagcatcattggtgctatagcgcggcagttcaagtttt 
               
               
                 cccgggtatgcaaaccgaaatcctcacttgaagagacggaagttctgtttgtattcattgggtacgat 
               
               
                 cgcaaggcccgtacgcacaatccttacaagctttcatcaaccttgaccaacatttatacaggttccag 
               
               
                 actccacgaagccggatgtgcaccctcatatcatgtggtgcgaggggatattgccacggccaccgaag 
               
               
                 gagtgattataaatgctgctaacagcaaaggacaacctggcggaggggtgtgcggagcgctgtataag 
               
               
                 aaattcccggaaagcttcgatttacagccgatcgaagtaggaaaagcgcgactggtcaaaggtgcagc 
               
               
                 taaacatatcattcatgccgtaggaccaaacttcaacaaagtttcggaggttgaaggtgacaaacagt 
               
               
                 tggcagaggcttatgagtccatcgctaagattgtcaacgataacaattacaagtcagtagcgattcca 
               
               
                 ctgttgtccaccggcatcttttccgggaacaaagatcgactaacccaatcattgaaccatttgctgac 
               
               
                 agctttagacaccactgatgcagatgtagccatatactgcagggacaagaaatgggaaatgactctca 
               
               
                 aggaagcagtggctaggagagaagcagtggaggagatatgcatatccgacgactcttcagtgacagaa 
               
               
                 cctgatgcagagctggtgagggtgcatccgaagagttctttggctggaaggaagggctacagcacaag 
               
               
                 cgatggcaaaactttctcatatttggaagggaccaagtttcaccaggcggccaaggatatagcagaaa 
               
               
                 ttaatgccatgtggcccgttgcaacggaggccaatgagcaggtatgcatgtatatcctcggagaaagc 
               
               
                 atgagcagtattaggtcgaaatgccccgtcgaagagtcggaagcctccacaccacctagcacgctgcc 
               
               
                 ttgcttgtgcatccatgccatgactccagaaagagtacagcgcctaaaagcctcacgtccagaacaaa 
               
               
                 ttactgtgtgctcatcctttccattgccgaagtatagaatcactggtgtgcagaagatccaatgctcc 
               
               
                 cagcctatattgttctcaccgaaagtgcctgcgtatattcatccaaggaagtatctcgtggaaacacc 
               
               
                 accggtagacgagactccggagccatcggcagagaaccaatccacagaggggacacctgaacaaccac 
               
               
                 cacttataaccgaggatgagaccaggactagaacgcctgagccgatcatcatcgaagaggaagaagag 
               
               
                 gatagcataagtttgctgtcagatggcccgacccaccaggtgctgcaagtcgaggcagacattcacgg 
               
               
                 gccgccctctgtatctagctcatcctggtccattcctcatgcatccgactttgatgtggacagtttat 
               
               
                 ccatacttgacaccctggagggagctagcgtgaccagcggggcaacgtcagccgagactaactcttac 
               
               
                 ttcgcaaagagtatggagtttctggcgcgaccggtgcctgcgcctcgaacagtattcaggaaccctcc 
               
               
                 acatcccgctccgcgcacaagaacaccgtcacttgcacccagcagggcctgctcgagaaccagcctag 
               
               
                 tttccaccccgccaggcgtgaatagggtgatcactagagaggagctcgaggcgcttaccccgtcacgc 
               
               
                 actcctagcaggtcggtctcgagaaccagcctggtctccaacccgccaggcgtaaatagggtgattac 
               
               
                 aagagaggagtttgaggcgttcgtagcacaacaacaatgacggtttgatgcgggtgcatacatctttt 
               
               
                 cctccgacaccggtcaagggcatttacaacaaaaatcagtaaggcaaacggtgctatccgaagtggtg 
               
               
                 ttggagaggaccgaattggagatttcgtatgccccgcgcctcgaccaagaaaaagaagaattactacg 
               
               
                 caagaaattacagttaaatcccacacctgctaacagaagcagataccagtccaggaaggtggagaaca 
               
               
                 tgaaagccataacagctagacgtattctgcaaggcctagggcattatttgaaggcagaaggaaaagtg 
               
               
                 gagtgctaccgaaccctgcatcctgttcctttgtattcatctagtgtgaaccgtgccttttcaagccc 
               
               
                 caaggtcgcagtggaagcctgtaacgccatgttgaaagagaactttccgactgtggcttcttactgta 
               
               
                 ttattccagagtacgatgcctatttggacatggttgacggagcttcatgctgcttagacactgccagt 
               
               
                 ttttgccctgcaaagctgcgcagctttccaaagaaacactcctatttggaacccacaatacgatcggc 
               
               
                 agtgccttcagcgatccagaacacgctccagaacgtcctggcagctgccacaaaaagaaattgcaatg 
               
               
                 tcacgcaaatgagagaattgcccgtattggattcggcggcctttaatgtggaatgcttcaagaaatat 
               
               
                 gcgtgtaataatgaatattgggaaacgtttaaagaaaaccccatcaggcttactgaagaaaacgtggt 
               
               
                 aaattacattaccaaattaaaaggaccaaaagctgctgctctttttgcgaagacacataatttgaata 
               
               
                 tgttgcaggacataccaatggacaggtttgtaatggacttaaagagagacgtgaaagtgactccagga 
               
               
                 acaaaacatactgaagaacggcccaaggtacaggtgatccaggctgccgatccgctagcaacagcgta 
               
               
                 tctgtgcggaatccaccgagagctggttaggagattaaatgcggtcctgcttccgaacattcatacac 
               
               
                 tgtttgatatgtcggctgaagactttgacgctattatagccgagcacttccagcctggggattgtgtt 
               
               
                 ctggaaactgacatcgcgtcgtttgataaaagtgaggacgacgccatggctctgaccgcgttaatgat 
               
               
                 tctggaagacttaggtgtggacgcagagctgttgacgctgattgaggcggctttcggcgaaatttcat 
               
               
                 caatacatttgcccactaaaactaaatttaaattcggagccatgatgaaatctggaatgttcctcaca 
               
               
                 ctgtttgtgaacacagtcattaacattgtaatcgcaagcagagtgttgagagaacggctaaccggatc 
               
               
                 accatgtgcagcattcattggagatgacaatatcgtgaaaggagtcaaatcggacaaattaatggcag 
               
               
                 acaggtgcgccacctggttgaatatggaagtcaagattatagatgctgtggtgggcgagaaagcgcct 
               
               
                 tatttctgtggagggtttattttgtgtgactccgtgaccggcacagcgtgccgtgtggcagaccccct 
               
               
                 aaaaaggctgtttaagcttggcaaacctctggcagcagacgatgaacatgatgatgacaggagaaggg 
               
               
                 cattgcatgaagagtcaacacgctggaaccgagtgggtattctttcagagctgtgcaaggcagtagaa 
               
               
                 tcaaggtatgaaaccgtaggaacttccatcatagttatggccatgactactctagctagcagtgttaa 
               
               
                 atcattcagctacctgagaggggcccctataactctctacggctaacctgaatggactacgacatagt 
               
               
                 ctagtcgagtctagtcgacgccaccatgggcaccgtgaacaagcctgtcgtgggcgtgctgatgggct 
               
               
                 tcggcatcatcaccggcaccctgagaatcaccaaccctgtgcgggccagcgtgctgagatacgacgac 
               
               
                 ttccacatcgacgaggacaagctggacaccaacagcgtgtacgagccctactaccacagcgaccacgc 
               
               
                 cgagagcagctgggtcaacagaggcgagagcagccggaaggcctacgaccacaacagcccctacatct 
               
               
                 ggccccggaacgactacgacggcttcctggaaaacgcccacgagcaccacggcgtgtacaatcagggc 
               
               
                 agaggcatcgacagcggcgagagactgatgcagcccacacagatgagcgcccaggaagatctgggcga 
               
               
                 cgacacaggcatccacgtgatccccaccctgaacggcgacgaccggcacaagatcgtgaacgtggacc 
               
               
                 agcggcagtacggcgacgtgttcaagggcgacctgaaccctaagccccagggccagagactgatcgag 
               
               
                 gtgtccgtggaagagaaccaccccttcaccctgagagcccccatccagagaatctacggcgtgcggta 
               
               
                 taccgagacttggagcttcctgcccagcctgacctgtacaggcgacgccgctcctgccatccagcaca 
               
               
                 tctgcctgaagcacaccacctgtttccaggacgtggtggtggacgtggactgcgccgagaacaccaaa 
               
               
                 gaggaccagctggccgagatcagctaccggttccagggcaagaaagaggccgaccagccctggatcgt 
               
               
                 ggtcaataccagcaccctgttcgacgagctggaactggacccccccgagattgaacccggcgtgctga 
               
               
                 aggtgctgcggaccgagaagcagtacctgggcgtgtacatctggaacatgcggggctccgacggcacc 
               
               
                 tctacctacgccaccttcctggtcacatggaagggcgacgagaaaacccggaaccctacccctgccgt 
               
               
                 gacccctcagcctagaggcgccgagttccatatgtggaattaccactcccacgtgttcagcgtgggcg 
               
               
                 acaccttcagcctggccatgcatctgcagtacaagatccacgaggcccccttcgacctgctgctggaa 
               
               
                 tggctgtacgtgcccatcgaccctacctgccagcccatgcggctgtacagcacctgtctgtaccaccc 
               
               
                 caacgcccctcagtgcctgagccacatgaacagcggctgcaccttcaccagccctcacctggctcaga 
               
               
                 gggtggccagcaccgtgtaccagaattgcgagcacgccgacaactacaccgcctactgcctgggcatc 
               
               
                 agccacatggaacccagcttcggcctgatcctgcacgatggcggcaccaccctgaagttcgtggacac 
               
               
                 acccgagagcctgagcggcctgtacgtgttcgtggtgtacttcaacggccacgtggaagccgtggcct 
               
               
                 acaccgtggtgtccaccgtggaccacttcgtgaacgccatcgaggaaagaggcttcccacccacagcc 
               
               
                 ggacagcctccagccaccaccaagcccaaagaaatcacccccgtgaaccccggcaccagccccctgct 
               
               
                 gagatatgctgcttggacaggcggactggccgctgtggtgctgctgtgcctggtcatcttcctgatct 
               
               
                 gcaccgccaagcggatgagagtgaaggcctaccgggtggacaagtccccctacaaccagagcatgtac 
               
               
                 tacgccggcctgcccgtggacgatttcgaggatagcgagagcaccgacaccgaggaagagttcggcaa 
               
               
                 cgccatcggcggatctcacggcggcagcagctacaccgtgtacatcgacaagaccagataatctagac 
               
               
                 gtcgcgaccacccaggatccgcctataactctctacggctaacctgaatggactacgacatagtctag 
               
               
                 tcgacgccaccatgtttctgatccagtgcctgatcagcgccgtgatcttctatattcaagtcacaaac 
               
               
                 gccctgatctttaagggcgaccacgtgtcactgcaggtcaacagcagcctgaccagcatcctgatccc 
               
               
                 catgcagaacgacaattacaccgagatcaagggccagctggtgttcatcggcgagcagctgcccaccg 
               
               
                 gcaccaattacagcggcaccctggaactgctgtacgccgataccgtggccttctgcttcagaagcgtg 
               
               
                 caggtcatcagatacgacggctgcccccggatcagaaccagcgccttcatcagctgccggtacaagca 
               
               
                 cagctggcactacggcaacagcaccgaccggatcagcaccgaacctgatgccggcgtgatgctgaaga 
               
               
                 tcaccaagcccggcatcaacgacgccggcgtgtacgtgctgctcgtgcggctggatcacagcagaagc 
               
               
                 accgacggcttcatcctgggcgtgaacgtgtacaccgccggcagccaccacaacatccacggcgtgat 
               
               
                 ctacaccagccccagcctgcagaacggctacagcaccagagccctgttccagcaggccagactgtgcg 
               
               
                 atctgcccgccacacctaagggcagcggcacaagcctgtttcagcacatgctggacctgagagccggc 
               
               
                 aagagcctggaagataacccctggctgcacgaggacgtggtcaccaccgagacaaagagcgtggtcaa 
               
               
                 agagggcatcgagaaccacgtgtaccccaccgacatgagcaccctgcccgagaagtccctgaacgacc 
               
               
                 cccctgagaacctgctgatcatcatccccatcgtggccagcgtgatgatcctgaccgccatggtcatc 
               
               
                 gtgatcgtgatcagcgtgaagcggcggagaatcaagaagcaccccatctaccggcccaacaccaagac 
               
               
                 cagacggggcatccagaacgccacccctgagtccgacgtgatgctggaagccgccattgcccagctgg 
               
               
                 ccaccatcagagaggaaagcccccctcacagcgtcgtgaaccccttcgtgaagtaatctagacgcggc 
               
               
                 cgcatacagcagcaattggcaagctgcttacatagaactcgcggcgattggcatgccgccttaaaatt 
               
               
                 tttattttatttttcttttcttttccgaatcggattttgtttttaatatttcaaaaaaaaaaaaaaaa 
               
               
                 aaaaaaaaaaaaaaaaaaagggtcggcatggcatctccacctcctcgcggtccgacctgggcatccga 
               
               
                 aggaggacgcacgtccactcggatggctaagggagagccacgtttaaaccagctccaattcgccctat 
               
               
                 agtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgt 
               
               
                 tacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgca 
               
               
                 ccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcgcatta 
               
               
                 agcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcc 
               
               
                 tttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggc 
               
               
                 tccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggt 
               
               
                 tcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaa 
               
               
                 tagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataag 
               
               
                 ggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaatttt 
               
               
                 aacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgt 
               
               
                 ttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaata 
               
               
                 atattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcat 
               
               
                 tttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggt 
               
               
                 gcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaaga 
               
               
                 acgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccg 
               
               
                 ggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcaca 
               
               
                 gaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataa 
               
               
                 cactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaaca 
               
               
                 tgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgag 
               
               
                 cgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttac 
               
               
                 tctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgct 
               
               
                 cggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatc 
               
               
                 attgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggc 
               
               
                 aactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgt 
               
               
                 cagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctag 
               
               
                 gtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtc 
               
               
                 agaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgc 
               
               
                 aaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccg 
               
               
                 aaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggcca 
               
               
                 ccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctg 
               
               
                 ccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcgg 
               
               
                 tcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagata 
               
               
                 cctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaa 
               
               
                 gcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagt 
               
               
                 cctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcct 
               
               
                 atggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgt 
               
               
                 tctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgct 
               
               
                 cgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaa 
               
               
                 accgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag 
               
               
                 cgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacacttt 
               
               
                 atgctcccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatga 
               
               
                 ccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctgggtaccgggcccacgc 
               
               
                 gtaatacgactcactatag_13775 
               
               
                   
               
               
                 VEE-based replicon encoding eGFP (SEQ ID NO: 80) 
               
               
                                                                           nsP1 
               
               
                                                                     ~~~~~~~~~~~~~~~~~ 
               
               
                     1      ATAGGCGGCG CATGAGAGAA GCCCAGACCA ATTACCTACC CAAAATGGAG AAAGTTCACG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                    61      TTGACATCGA GGAAGACAGC CCATTCCTCA GAGCTTTGCA GCGGAGCTTC CCGCAGTTTG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   121      AGGTAGAAGC CAAGCAGGTC ACTGATAATG ACCATGCTAA TGCCAGAGCG TTTTCGCATC 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   181      TGGCTTCAAA ACTGATCGAA ACGGAGGTGG ACCCATCCGA CACGATCCTT GACATTGGAA 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   241      GTGCGCCCGC CCGCAGAATG TATTCTAAGC ACAAGTATCA TTGTATCTGT CCGATGAGAT 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   301      GTGCGGAAGA TCCGGACAGA TTGTATAAGT ATGCAACTAA GCTGAAGAAA AACTGTAAGG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   361      AAATAACTGA TAAGGAATTG GACAAGAAAA TGAAGGAGCT CGCCGCCGTC ATGAGCGACC 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   421      CTGACCTGGA AACTGAGACT ATGTGCCTCC ACGACGACGA GTCGTGTCGC TACGAAGGGC 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   481      AAGTCGCTGT TTACCAGGAT GTATACGCGG TTGACGGACC GACAAGTCTC TATCACCAAG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   541      CCAATAAGGG AGTTAGAGTC GCCTACTGGA TAGGCTTTGA CACCACCCCT TTTATGTTTA 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   601      AGAACTTGGC TGGAGCATAT CCATCATACT CTACCAACTG GGCCGACGAA ACCGTGTTAA 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   661      CGGCTCGTAA CATAGGCCTA TGCAGCTCTG ACGTTATGGA GCGGTCACGT AGAGGGATGT 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   721      CCATTCTTAG AAAGAAGTAT TTGAAACCAT CCAACAATGT TCTATTCTCT GTTGGCTCGA 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   781      CCATCTACCA CGAGAAGAGG GACTTACTGA GGAGCTGGCA CCTGCCGTCT GTATTTCACT 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   841      TACGTGGCAA GCAAAATTAC ACATGTCGGT GTGAGACTAT AGTTAGTTGC GACGGGTACG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   901      TCGTTAAAAG AATAGCTATC AGTCCAGGCC TGTATGGGAA GCCTTCAGGC TATGCTGCTA 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   961      CGATGCACCG CGAGGGATTC TTGTGCTGCA AAGTGACAGA CACATTGAAC GGGGAGAGGG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1021      TCTCTTTTCC CGTGTGCACG TATGTGCCAG CTACATTGTG TGACCAAATG ACTGGCATAC 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1081      TGGCAACAGA TGTCAGTGCG GACGACGCGC AAAAACTGCT GGTTGGGCTC AACCAGCGTA 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1141      TAGTCGTCAA CGGTCGCACC CAGAGAAACA CCAATACCAT GAAAAATTAC CTTTTGCCCG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1201      TAGTGGCCCA GGCATTTGCT AGGTGGGCAA AGGAATATAA GGAAGATCAA GAAGATGAAA 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1261      GGCCACTAGG ACTACGAGAT AGACAGTTAG TCATGGGGTG TTGTTGGGCT TTTAGAAGGC 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1321      ACAAGATAAC ATCTATTTAT AAGCGCCCGG ATACCCAAAC CATCATCAAA GTGAACAGCG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1381      ATTTCCACTC ATTCGTGCTG CCCAGGATAG GCAGTAACAC ATTGGAGATC GGGCTGAGAA 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1441      CAAGAATCAG GAAAATGTTA GAGGAGCACA AGGAGCCGTC ACCTCTCATT ACCGCCGAGG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1501      ACGTACAAGA AGCTAAGTGC GCAGCCGATG AGGCTAAGGA GGTGCGTGAA GCCGAGGAGT 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1561      TGCGCGCAGC TCTACCACCT TTGGCAGCTG ATGTTGAGGA GCCCACTCTG GAAGCCGATG 
               
               
                                                                 nsP2 
               
               
                                                    ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1621      TAGACTTGAT GTTACAAGAG GCTGGGGCCG GCTCAGTGGA GACACCTCGT GGCTTGATAA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1681      AGGTTACCAG CTACGATGGC GAGGACAAGA TCGGCTCTTA CGCTGTGCTT TCTCCGCAGG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1741      CTGTACTCAA GAGTGAAAAA TTATCTTGCA TCCACCCTCT CGCTGAACAA GTCATAGTGA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1801      TAACACACTC TGGCCGAAAA GGGCGTTATG CCGTGGAACC ATACCATGGT AAAGTAGTGG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1861      TGCCAGAGGG ACATGCAATA CCCGTCCAGG ACTTTCAAGC TCTGAGTGAA AGTGCCACCA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1921      TTGTGTACAA CGAACGTGAG TTCGTAAACA GGTACCTGCA CCATATTGCC ACACATGGAG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1981      GAGCGCTGAA CACTGATGAA GAATATTACA AAACTGTCAA GCCCAGCGAG CACGACGGCG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2041      AATACCTGTA CGACATCGAC AGGAAACAGT GCGTCAAGAA AGAACTAGTC ACTGGGCTAG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2101      GGCTCACAGG CGAGCTGGTG GATCCTCCCT TCCATGAATT CGCCTACGAG AGTCTGAGAA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2161      CACGACCAGC CGCTCCTTAC CAAGTACCAA CCATAGGGGT GTATGGCGTG CCAGGATCAG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2221      GCAAGTCTGG CATCATTAAA AGCGCAGTCA CCAAAAAAGA TCTAGTGGTG AGCGCCAAGA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2281      AAGAAAACTG TGCAGAAATT ATAAGGGACG TCAAGAAAAT GAAAGGGCTG GACGTCAATG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2341      CCAGAACTGT GGACTCAGTG CTCTTGAATG GATGCAAACA CCCCGTAGAG ACCCTGTATA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2401      TTGACGAAGC TTTTGCTTGT CATGCAGGTA CTCTCAGAGC GCTCATAGCC ATTATAAGAC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2461      CTAAAAAGGC AGTGCTCTGC GGGGATCCCA AACAGTGCGG TTTTTTTAAC ATGATGTGCC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2521      TGAAAGTGCA TTTTAACCAC GAGATTTGCA CACAAGTCTT CCACAAAAGC ATCTCTCGCC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2581      GTTGCACTAA ATCTGTGACT TCGGTCGTCT CAACCTTGTT TTACGACAAA AAAATGAGAA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2641      CGACGAATCC GAAAGAGACT AAGATTGTGA TTGACACTAC CGGCAGTACC AAACCTAAGC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2701      AGGACGATCT CATTCTCACT TGTTTCAGAG GGTGGGTGAA GCAGTTGCAA ATAGATTACA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2761      AAGGCAACGA AATAATGACG GCAGCTGCCT CTCAAGGGCT GACCCGTAAA GGTGTGTATG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2821      CCGTTCGGTA CAAGGTGAAT GAAAATCCTC TGTACGCACC CACCTCAGAA CATGTGAACG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2881      TCCTACTGAC CCGCACGGAG GACCGCATCG TGTGGAAAAC ACTAGCCGGC GACCCATGGA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2941      TAAAAACACT GACTGCCAAG TACCCTGGGA ATTTCACTGC CACGATAGAG GAGTGGCAAG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3001      CAGAGCATGA TGCCATCATG AGGCACATCT TGGAGAGACC GGACCCTACC GACGTCTTCC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3061      AGAATAAGGC AAACGTGTGT TGGGCCAAGG CTTTAGTGCC GGTGCTGAAG ACCGCTGGCA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3121      TAGACATGAC CACTGAACAA TGGAACACTG TGGATTATTT TGAAACGGAC AAAGCTCACT 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3181      CAGCAGAGAT AGTATTGAAC CAACTATGCG TGAGGTTCTT TGGACTCGAT CTGGACTCCG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3241      GTCTATTTTC TGCACCCACT GTTCCGTTAT CCATTAGGAA TAATCACTGG GATAACTCCC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3301      CGTCGCCTAA CATGTACGGG CTGAATAAAG AAGTGGTCCG TCAGCTCTCT CGCAGGTACC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3361      CACAACTGCC TCGGGCAGTT GCCACTGGAA GAGTCTATGA CATGAACACT GGTACACTGC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3421      GCAATTATGA TCCGCGCATA AACCTAGTAC CTGTAAACAG AAGACTGCCT CATGCTTTAG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3481      TCCTCCACCA TAATGAACAC CCACAGAGTG ACTTTTCTTC ATTCGTCAGC AAATTGAAGG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3541      GCAGAACTGT CCTGGTGGTC GGGGAAAAGT TGTCCGTCCC AGGCAAAATG GTTGACTGGT 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3601      TGTCAGACCG GCCTGAGGCT ACCTTCAGAG CTCGGCTGGA TTTAGGCATC CCAGGTGATG 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3661      TGCCCAAATA TGACATAATA TTTGTTAATG TGAGGACCCC ATATAAATAC CATCACTATC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3721      AGCAGTGTGA AGACCATGCC ATTAAGCTTA GCATGTTGAC CAAGAAAGCT TGTCTGCATC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3781      TGAATCCCGG CGGAACCTGT GTCAGCATAG GTTATGGTTA CGCTGACAGG GCCAGCGAAA 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3841      GCATCATTGG TGCTATAGCG CGGCAGTTCA AGTTTTCCCG GGTATGCAAA CCGAAATCCT 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3901      CACTTGAAGA GACGGAAGTT CTGTTTGTAT TCATTGGGTA CGATCGCAAG GCCCGTACGC 
               
               
                                                 nsP2 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3961      ACAATCCTTA CAAGCTTTCA TCAACCTTGA CCAACATTTA TACAGGTTCC AGACTCCACG 
               
               
                                                        nsP3 
               
               
                                 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                        nsP2 
               
               
                     ~~~~~~~~~~~~ 
               
               
                  4021      AAGCCGGATG TGCACCCTCA TATCATGTGG TGCGAGGGGA TATTGCCACG GCCACCGAAG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4081      GAGTGATTAT AAATGCTGCT AACAGCAAAG GACAACCTGG CGGAGGGGTG TGCGGAGCGC 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4141      TGTATAAGAA ATTCCCGGAA AGCTTCGATT TACAGCCGAT CGAAGTAGGA AAAGCGCGAC 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4201      TGGTCAAAGG TGCAGCTAAA CATATCATTC ATGCCGTAGG ACCAAACTTC AACAAAGTTT 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4261      CGGAGGTTGA AGGTGACAAA CAGTTGGCAG AGGCTTATGA GTCCATCGCT AAGATTGTCA 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4321      ACGATAACAA TTACAAGTCA GTAGCGATTC CACTGTTGTC CACCGGCATC TTTTCCGGGA 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4381      ACAAAGATCG ACTAACCCAA TCATTGAACC ATTTGCTGAC AGCTTTAGAC ACCACTGATG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4441      CAGATGTAGC CATATACTGC AGGGACAAGA AATGGGAAAT GACTCTCAAG GAAGCAGTGG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4501      CTAGGAGAGA AGCAGTGGAG GAGATATGCA TATCCGACGA CTCTTCAGTG ACAGAACCTG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4561      ATGCAGAGCT GGTGAGGGTG CATCCGAAGA GTTCTTTGGC TGGAAGGAAG GGCTACAGCA 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4621      CAAGCGATGG CAAAACTTTC TCATATTTGG AAGGGACCAA GTTTCACCAG GCGGCCAAGG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4681      ATATAGCAGA AATTAATGCC ATGTGGCCCG TTGCAACGGA GGCCAATGAG CAGGTATGCA 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4741      TGTATATCCT CGGAGAAAGC ATGAGCAGTA TTAGGTCGAA ATGCCCCGTC GAAGAGTCGG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4801      AAGCCTCCAC ACCACCTAGC ACGCTGCCTT GCTTGTGCAT CCATGCCATG ACTCCAGAAA 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4861      GAGTACAGCG CCTAAAAGCC TCACGTCCAG AACAAATTAC TGTGTGCTCA TCCTTTCCAT 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4921      TGCCGAAGTA TAGAATCACT GGTGTGCAGA AGATCCAATG CTCCCAGCCT ATATTGTTCT 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4981      CACCGAAAGT GCCTGCGTAT ATTCATCCAA GGAAGTATCT CGTGGAAACA CCACCGGTAG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5041      ACGAGACTCC GGAGCCATCG GCAGAGAACC AATCCACAGA GGGGACACCT GAACAACCAC 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5101      CACTTATAAC CGAGGATGAG ACCAGGACTA GAACGCCTGA GCCGATCATC ATCGAAGAGG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5161      AAGAAGAGGA TAGCATAAGT TTGCTGTCAG ATGGCCCGAC CCACCAGGTG CTGCAAGTCG 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5221      AGGCAGACAT TCACGGGCCG CCCTCTGTAT CTAGCTCATC CTGGTCCATT CCTCATGCAT 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5281      CCGACTTTGA TGTGGACAGT TTATCCATAC TTGACACCCT GGAGGGAGCT AGCGTGACCA 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5341      GCGGGGCAAC GTCAGCCGAG ACTAACTCTT ACTTCGCAAA GAGTATGGAG TTTCTGGCGC 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5401      GACCGGTGCC TGCGCCTCGA ACAGTATTCA GGAACCCTCC ACATCCCGCT CCGCGCACAA 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5461      GAACACCGTC ACTTGCACCC AGCAGGGCCT GCTCGAGAAC CAGCCTAGTT TCCACCCCGC 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5521      CAGGCGTGAA TAGGGTGATC ACTAGAGAGG AGCTCGAGGC GCTTACCCCG TCACGCACTC 
               
               
                                                 nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5581      CTAGCAGGTC GGTCTCGAGA ACCAGCCTGG TCTCCAACCC GCCAGGCGTA AATAGGGTGA 
               
               
                                                                         nsP4 
               
               
                                                                  ~~~~~~~~~~~~~~~~~~~~ 
               
               
                                       nsP3 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5641      TTACAAGAGA GGAGTTTGAG GCGTTCGTAG CACAACAACA ATGACGGTTT GATGCGGGTG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5701      CATACATCTT TTCCTCCGAC ACCGGTCAAG GGCATTTACA ACAAAAATCA GTAAGGCAAA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5761      CGGTGCTATC CGAAGTGGTG TTGGAGAGGA CCGAATTGGA GATTTCGTAT GCCCCGCGCC 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5821      TCGACCAAGA AAAAGAAGAA TTACTACGCA AGAAATTACA GTTAAATCCC ACACCTGCTA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5881      ACAGAAGCAG ATACCAGTCC AGGAAGGTGG AGAACATGAA AGCCATAACA GCTAGACGTA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5941      TTCTGCAAGG CCTAGGGCAT TATTTGAAGG CAGAAGGAAA AGTGGAGTGC TACCGAACCC 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6001      TGCATCCTGT TCCTTTGTAT TCATCTAGTG TGAACCGTGC CTTTTCAAGC CCCAAGGTCG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6061      CAGTGGAAGC CTGTAACGCC ATGTTGAAAG AGAACTTTCC GACTGTGGCT TCTTACTGTA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6121      TTATTCCAGA GTACGATGCC TATTTGGACA TGGTTGACGG AGCTTCATGC TGCTTAGACA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6181      CTGCCAGTTT TTGCCCTGCA AAGCTGCGCA GCTTTCCAAA GAAACACTCC TATTTGGAAC 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6241      CCACAATACG ATCGGCAGTG CCTTCAGCGA TCCAGAACAC GCTCCAGAAC GTCCTGGCAG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6301      CTGCCACAAA AAGAAATTGC AATGTCACGC AAATGAGAGA ATTGCCCGTA TTGGATTCGG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6361      CGGCCTTTAA TGTGGAATGC TTCAAGAAAT ATGCGTGTAA TAATGAATAT TGGGAAACGT 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6421      TTAAAGAAAA CCCCATCAGG CTTACTGAAG AAAACGTGGT AAATTACATT ACCAAATTAA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6481      AAGGACCAAA AGCTGCTGCT CTTTTTGCGA AGACACATAA TTTGAATATG TTGCAGGACA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6541      TACCAATGGA CAGGTTTGTA ATGGACTTAA AGAGAGACGT GAAAGTGACT CCAGGAACAA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6601      AACATACTGA AGAACGGCCC AAGGTACAGG TGATCCAGGC TGCCGATCCG CTAGCAACAG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6661      CGTATCTGTG CGGAATCCAC CGAGAGCTGG TTAGGAGATT AAATGCGGTC CTGCTTCCGA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6721      ACATTCATAC ACTGTTTGAT ATGTCGGCTG AAGACTTTGA CGCTATTATA GCCGAGCACT 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6781      TCCAGCCTGG GGATTGTGTT CTGGAAACTG ACATCGCGTC GTTTGATAAA AGTGAGGACG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6841      ACGCCATGGC TCTGACCGCG TTAATGATTC TGGAAGACTT AGGTGTGGAC GCAGAGCTGT 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6901      TGACGCTGAT TGAGGCGGCT TTCGGCGAAA TTTCATCAAT ACATTTGCCC ACTAAAACTA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  6961      AATTTAAATT CGGAGCCATG ATGAAATCTG GAATGTTCCT CACACTGTTT GTGAACACAG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7021      TCATTAACAT TGTAATCGCA AGCAGAGTGT TGAGAGAACG GCTAACCGGA TCACCATGTG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7081      CAGCATTCAT TGGAGATGAC AATATCGTGA AAGGAGTCAA ATCGGACAAA TTAATGGCAG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7141      ACAGGTGCGC CACCTGGTTG AATATGGAAG TCAAGATTAT AGATGCTGTG GTGGGCGAGA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7201      AAGCGCCTTA TTTCTGTGGA GGGTTTATTT TGTGTGACTC CGTGACCGGC ACAGCGTGCC 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7261      GTGTGGCAGA CCCCCTAAAA AGGCTGTTTA AGCTTGGCAA ACCTCTGGCA GCAGACGATG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7321      AACATGATGA TGACAGGAGA AGGGCATTGC ATGAAGAGTC AACACGCTGG AACCGAGTGG 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7381      GTATTCTTTC AGAGCTGTGC AAGGCAGTAGA ATCAAGGTA TGAAACCGTA GGAACTTCCA 
               
               
                                                 nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7441      TCATAGTTAT GGCCATGACT ACTCTAGCTA GCAGTGTTAA ATCATTCAGC TACCTGAGAG 
               
               
                                    subgenomic promoter 
               
               
                                   ~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                nsP4 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7501      GGGCCCCTAT AACTCTCTAC GGCTAACCTG AATGGACTAC GACATAGTCT AGTCGACGCC 
               
               
                                                  eGFP 
               
               
                        ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7561      ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7621      GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7681      TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7741      ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7801      AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7861      TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7921      CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  7981      CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8041      AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8101      GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8161      CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG 
               
               
                                                 eGFP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8221      GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG 
               
               
                     eGFP                                            3′UTR 
               
               
                    ~~~~~~                                 ~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8281      TGATAATCTA GACGGCGCGC CCACCCAGCG GCCGCATACA GCAGCAATTG GCAAGCTGCT 
               
               
                                                3′UTR    
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8341      TACATAGAAC TCGCGGCGAT TGGCATGCCG CCTTAAAATT TTTATTTTAT TTTTCTTTTC 
               
               
                                  3′UTR 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8401      TTTTCCGAAT CGGATTTTGT TTTTAATATT TCAAAAAAAA AAAAAAAAAA AAAAAAAAAA 
               
               
                                                HDV ribozyme 
               
               
                            ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8461      AAAAAAAGGG TCGGCATGGC ATCTCCACCT CCTCGCGGTC CGACCTGGGC ATCCGAAGGA 
               
               
                                 HDV ribozyme 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  8521      GGACGCACGT CCACTCGGAT GGCTAAGGGA GAGCCACGTT TAAACCAGCT CCAATTCGCC 
               
               
                  8581      CTATAGTGAG TCGTATTACG CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA 
               
               
                  8641      AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 
               
               
                  8701      TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 
               
               
                  8761      ATGGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT 
               
               
                  8821      GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT 
               
               
                  8881      CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG 
               
               
                  8941      ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG 
               
               
                  9001      TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 
               
               
                  9061      TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 
               
               
                  9121      TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 
               
               
                  9181      ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAGGTGGCAC TTTTCGGGGA 
               
               
                  9241      AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC 
               
               
                                                                                bla 
               
               
                                                                             ~~~~~~~~~ 
               
               
                  9301      ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9361      CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9421      CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9481      TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9541      TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9601      GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9661      TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9721      GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9781      AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9841      GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9901      ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  9961      CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                 10021      CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                 10081      ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG 
               
               
                                                 bla 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                 10141      AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT 
               
               
                        bla 
               
               
                     ~~~~~~~~~ 
               
               
                 10201      AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT 
               
               
                 10261      CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC 
               
               
                 10321      CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 
               
               
                 10381      TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 
               
               
                 10441      CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC 
               
               
                 10501      TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 
               
               
                 10561      TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT 
               
               
                 10621      GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 
               
               
                 10681      AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 
               
               
                 10741      ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA 
               
               
                 10801      GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 
               
               
                 10861      GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA 
               
               
                 10921      CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 
               
               
                 10981      AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT 
               
               
                 11041      GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT 
               
               
                 11101      CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA 
               
               
                 11161      ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG 
               
               
                 11221      TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT 
               
               
                 11281      TAGGCACCCC AGGCTTTACA CTTTATGCTC CCGGCTCGTA TGTTGTGTGG AATTGTGAGC 
               
               
                 11341      GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTAAC 
               
               
                 11401      CCTCACTAAA GGGAACAAAA GCTGGGTACC GGGCCCACGC GTAATACGAC TCACTATAG 
               
               
                   
               
               
                 VEE cap helper 
               
               
                                        5′UTR 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                                           nsP1 
               
               
                                                                     ~~~~~~~~~~~~~~~~~ 
               
               
                     1      ATAGGCGGCG CATGAGAGAA GCCCAGACCA ATTACCTACC CAAATAGGAG AAAGTTCACG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                    61      TTGACATCGA GGAAGACAGC CCATTCCTCA GAGCTTTGCA GCGGAGCTTC CCGCAGTTTG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   121      AGGTAGAAGC CAAGCAGGTC ACTGATAATG ACCATGCTAA TGCCAGAGCG TTTTCGCATC 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   181      TGGCTTCAAA ACTGATCGAA ACGGAGGTGG ACCCATCCGA CACGATCCTT GACATTGGAC 
               
               
                                                    VEECAP 
               
               
                              ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   241      GGACCGACCA TGTTCCCGTT CCAGCCAATG TATCCGATGC AGCCAATGCC CTATCGCAAC 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   301      CCGTTCGCGG CCCCGCGCAG GCCCTGGTTC CCCAGAACCG ACCCTTTTCT GGCGATGCAG 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   361      GTGCAGGAAT TAACCCGCTC GATGGCTAAC CTGACGTTCA AGCAACGCCG GGACGCGCCA 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   421      CCTGAGGGGC CATCCGCTAA GAAACCGAAG AAGGAGGCCT CGCAAAAACA GAAAGGGGGA 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   481      GGCCAAGGGA AGAAGAAGAA GAACCAAGGG AAGAAGAAGG CTAAGACAGG GCCGCCTAAT 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   541      CCGAAGGCAC AGAATGGAAA CAAGAAGAAG ACCAACAAGA AACCAGGCAA GAGACAGCGC 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   601      ATGGTCATGA AATTGGAATC TGACAAGACG TTCCCAATCA TGTTGGAAGG GAAGATAAAC 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                                   H152G 
               
               
                                                                   ~~~ 
               
               
                   661      GGCTACGCTT GTGTGGTCGG AGGGAAGTTA TTCAGGCCGA TGGGTGTGGA AGGCAAGATC 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   721      GACAACGACG TTCTGGCCGC GCTTAAGACG AAGAAAGCAT CCAAATACGA TCTTGAGTAT 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   781      GCAGATGTGC CACAGAACAT GCGGGCCGAT ACATTCAAAT ACACCCATGA GAAACCCCAA 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   841      GGCTATTACA GCTGGCATCA TGGAGCAGTC CAATATGAAA ATGGGCGTTT CACGGTGCCG 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   901      AAAGGAGTTG GGGCCAAGGG AGACAGCGGA CGACCCATTC TGGATAACCA GGGACGGGTG 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   961      GTCGCTATTG TGCTGGGAGG TGTGAATGAA GGATCTAGGA CAGCCCTTTC AGTCGTCATG 
               
               
                                                VEECAP 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1021      TGGAACGAGA AGGGAGTTAC CGTGAAGTAT ACTCCGGAGA ACTGCGAGCA ATGGTAATAG 
               
               
                     VEECAP                            3′UTR 
               
               
                     ~~~         ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1081      TAAGCGGCCG CATACAGCAG CAATTGGCAA GCTGCTTACA TAGAACTCGC GGCGATTGGC 
               
               
                                                3′UTR 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1141      ATGCCGCCTT AAAATTTTTA TTTTATTTTT CTTTTCTTTT CCGAATCGGA TTTTGTTTTT 
               
               
                      3′UTR                                           HDV ribozyme 
               
               
                     ~~~~~~~~~                                   
               
               
                  1201      AATATTTCAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAGGGTCGG CATGGCATCT 
               
               
                                             HDV ribozyme 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1261      CCACCTCCTC GCGGTCCGAC CTGGGCATCC GAAGGAGGAC GCACGTCCAC TCGGATGGCT 
               
               
                     HDV ribozyme 
               
               
                     ~~~~~~~~~~~~~~ 
               
               
                  1321      AAGGGAGAGC CACGTTTAAA CACGTGATAT CTGGCCTCAT GGGCCTTCCT TTCACTGCCC 
               
               
                  1381      GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAC ATGGTCATAG CTGTTTCCTT 
               
               
                  1441      GCGTATTGGG CGCTCTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGGTA 
               
               
                                                          colE1 
               
               
                                       ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1501      AAGCCTGGGG TGCCTAATGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1561      CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1621      CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1681      GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1741      TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1801      AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1861      CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1921      CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1981      TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2041      TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2101      CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
               
               
                               colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2161      AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
               
               
                  2221      AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
               
               
                  2281      AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTATTAGA 
               
               
                                                                              ~~~ 
               
               
                                                                                KanR 
               
               
                  2341      AAAATTCATC CAGCAGACGA TAAAACGCAA TACGCTGGCT ATCCGGTGCC GCAATGCCAT 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2401      ACAGCACCAG AAAACGATCC GCCCATTCGC CGCCCAGTTC TTCCGCAATA TCACGGGTGG 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2461      CCAGCGCAAT ATCCTGATAA CGATCCGCCA CGCCCAGACG GCCGCAATCA ATAAAGCCGC 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2521      TAAAACGGCC ATTTTCCACC ATAATGTTCG GCAGGCACGC ATCACCATGG GTCACCACCA 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2581      GATCTTCGCC ATCCGGCATG CTCGCTTTCA GACGCGCAAA CAGCTCTGCC GGTGCCAGGC 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2641      CCTGATGTTC TTCATCCAGA TCATCCTGAT CCACCAGGCC CGCTTCCATA CGGGTACGCG 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2701      CACGTTCAAT ACGATGTTTC GCCTGATGAT CAAACGGACA GGTCGCCGGG TCCAGGGTAT 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2761      GCAGACGACG CATGGCATCC GCCATAATGC TCACTTTTTC TGCCGGCGCC AGATGGCTAG 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2821      ACAGCAGATC CTGACCCGGC ACTTCGCCCA GCAGCAGCCA ATCACGGCCC GCTTCGGTCA 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2881      CCACATCCAG CACCGCCGCA CACGGAACAC CGGTGGTGGC CAGCCAGCTC AGACGCGCCG 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  2941      CTTCATCCTG CAGCTCGTTC AGCGCACCGC TCAGATCGGT TTTCACAAAC AGCACCGGAC 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  3001      GACCCTGCGC GCTCAGACGA AACACCGCCG CATCAGAGCA GCCAATGGTC TGCTGCGCCC 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  3061      AATCATAGCC AAACAGACGT TCCACCCACG CTGCCGGGCT ACCCGCATGC AGGCCATCCT 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  3121      GTTCAATCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
               
               
                     ~~~~~~~~~~~ 
               
               
                        KanR 
               
               
                  3181      TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
               
               
                  3241      TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA 
               
               
                  3301      TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA 
               
               
                  3361      ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGGCCGCTAC AGGGCGCTCC CATTCGCCAT 
               
               
                  3421      TCAGGCTGCG CAACTGTTGG GAAGGGCGTT TCGGTGCGGG CCTCTTCGCT ATTACGCCAG 
               
               
                  3481      CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG 
               
               
                                 T7 promoter 
               
               
                              ~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3541      TCACACGCGT AATACGACTC ACTATAG 
               
               
                   
               
               
                 VEE gly helper 
               
               
                                        5′UTR 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                                           nsP1 
               
               
                                                                     ~~~~~~~~~~~~~~~~~ 
               
               
                     1      ATAGGCGGCG CATGAGAGAA GCCCAGACCA ATTACCTACC CAAATAGGAG AAAGTTCACG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                    61      TTGACATCGA GGAAGACAGC CCATTCCTCA GAGCTTTGCA GCGGAGCTTC CCGCAGTTTG 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   121      AGGTAGAAGC CAAGCAGGTC ACTGATAATG ACCATGCTAA TGCCAGAGCG TTTTCGCATC 
               
               
                                                 nsP1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   181      TGGCTTCAAA ACTGATCGAA ACGGAGGTGG ACCCATCCGA CACGATCCTT GACATTGGAC 
               
               
                                                    VEE GLY 
               
               
                              ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   241      GGACCGACCA TGTCACTAGT GACCACCATG TGTCTGCTCG CCAATGTGAC GTTCCCATGT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   301      GCTCAACCAC CAATTTGCTA CGACAGAAAA CCAGCAGAGA CTTTGGCCAT GCTCAGCGTT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   361      AACGTTGACA ACCCGGGCTA CGATGAGCTG CTGGAAGCAG CTGTTAAGTG CCCCGGAAGG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   421      AAAAGGAGAT CCACCGAGGA GCTGTTTAAT GAGTATAAGC TAACGCGCCC TTACATGGCC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   481      AGATGCATCA GATGTGCAGT TGGGAGCTGC CATAGTCCAA TAGCAATCGA GGCAGTAAAG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   541      AGCGACGGGC ACGACGGTTA TGTTAGACTT CAGACTTCCT CGCAGTATGG CCTGGATTCC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   601      TCCGGCAACT TAAAGGGCAG GACCATGCGG TATGACATGC ACGGGACCAT TAAAGAGATA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   661      CCACTACATC AAGTGTCACT CTATACATCT CGCCCGTGTC ACATTGTGGA TGGGCACGGT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   721      TATTTCCTGC TTGCCAGGTG CCCGGCAGGG GACTCCATCA CCATGGAATT TAAGAAAGAT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   781      TCCGTCAGAC ACTCCTGCTC GGTGCCGTAT GAAGTGAAAT TTAATCCTGT AGGCAGAGAA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   841      CTCTATACTC ATCCCCCAGA ACACGGAGTA GAGCAAGCGT GCCAAGTCTA CGCACATGAT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   901      GCACAGAACA GAGGAGCTTA TGTCGAGATG CACCTCCCGG GCTCAGAAGT GGACAGCAGT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                   961      TTGGTTTCCT TGAGCGGCAG TTCAGTCACC GTGACACCTC CTGATGGGAC TAGCGCCCTG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1021      GTGGAATGCG AGTGTGGCGG CACAAAGATC TCCGAGACCA TCAACAAGAC AAAACAGTTC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1081      AGCCAGTGCA CAAAGAAGGA GCAGTGCAGA GCATATCGGC TGCAGAACGA TAAGTGGGTG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1141      TATAATTCTG ACAAACTGCC CAAAGCAGCG GGAGCCACCT TAAAAGGAAA ACTGCATGTC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1201      CCATTCTTGC TGGCAGACGG CAAATGCACC GTGCCTCTAG CACCAGAACC TATGATAACC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1261      TTCGGTTTCA GATCAGTGTC ACTGAAACTG CACCCTAAGA ATCCCACATA TCTAATCACC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1321      CGCCAACTTG CTGATGAGCC TCACTACACG CACGAGCTCA TATCTGAACC AGCTGTTAGG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1381      AATTTTACCG TCACCGAAAA AGGGTGGGAG TTTGTATGGG GAAACCACCC GCCGAAAAGG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1441      TTTTGGGCAC AGGAAACAGC ACCCGGAAAT CCACATGGGC TACCGCACGA GGTGATAACT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1501      CATTATTACC ACAGATACCC TATGTCCACC ATCCTGGGTT TGTCAATTTG TGCCGCCATT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1561      GCAACCGTTT CCGTTGCAGC GTCTACCTGG CTGTTTTGCA GATCTAGAGT TGCGTGCCTA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1621      ACTCCTTACC GGCTAACACC TAACGCTAGG ATACCATTTT GTCTGGCTGT GCTTTGCTGC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1681      GCCCGCACTG CCCGGGCCGA GACCACCTGG GAGTCCTTGG ATCACCTATG GAACAATAAC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1741      CAACAGATGT TCTGGATTCA ATTGCTGATC CCTCTGGCCG CCTTGATCGT AGTGACTCGC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1801      CTGCTCAGGT GCGTGTGCTG TGTCGTGCCT TTTTTAGTCA TGGCCGGCGC CGCAGGCGCC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1861      GGCGCCTACG AGCACGCGAC CACGATGCCG AGCCAAGCGG GAATCTCGTA TAACACTATA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1921      GTCAACAGAG CAGGCTACGC ACCACTCCCT ATCAGCATAA CACCAACAAA GATCAAGCTG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  1981      ATACCTACAG TGAACTTGGA GTACGTCACC TGCCACTACA AAACAGGAAT GGATTCACCA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2041      GCCATCAAAT GCTGCGGATC TCAGGAATGC ACTCCAACTT ACAGGCCTGA TGAACAGTGC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2101      AAAGTCTTCA CAGGGGTTTA CCCGTTCATG TGGGGTGGTG CATATTGCTT TTGCGACACT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2161      GAGAACACCC AAGTCAGCAA GGCCTACGTA ATGAAATCTG ACGACTGCCT TGCGGATCAT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2221      GCTGAAGCAT ATAAAGCGCA CACAGCCTCA GTGCAGGCGT TCCTCAACAT CACAGTGGGA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2281      GAACACTCTA TTGTGACTAC CGTGTATGTG AATGGAGAAA CTCCTGTGAA TTTCAATGGG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2341      GTCAAAATAA CTGCAGGTCC GCTTTCCACA GCTTGGACAC CCTTTGATCG CAAAATCGTG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2401      CAGTATGCCG GGGAGATCTA TAATTATGAT TTTCCTGAGT ATGGGGCAGG ACAACCAGGA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2461      GCATTTGGAG ATATACAATC CAGAACAGTC TCAAGCTCTG ATCTGTATGC CAATACCAAC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2521      CTAGTGCTGC AGAGACCCAA AGCAGGAGCG ATCCACGTGC CATACACTCA GGCACCTTCG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2581      GGTTTTGAGC AATGGAAGAA AGATAAAGCT CCATCATTGA AATTTACCGC CCCTTTCGGA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2641      TGCGAAATAT ATACAAACCC CATTCGCGCC GAAAACTGTG CTGTAGGGTC AATTCCATTA 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2701      GCCTTTGACA TTCCCGACGC CTTGTTCACC AGGGTGTCAG AAACACCGAC ACTTTCAGCG 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2761      GCCGAATGCA CTCTTAACGA GTGCGTGTAT TCTTCCGACT TTGGTGGGAT CGCCACGGTC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2821      AAGTACTCGG CCAGCAAGTC AGGCAAGTGC GCAGTCCATG TGCCATCAGG GACTGCTACC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2881      CTAAAAGAAG CAGCAGTCGA GCTAACCGAG CAAGGGTCGG CGACTATCCA TTTCTCGACC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  2941      GCAAATATCC ACCCGGAGTT CAGGCTCCAA ATATGCACAT CATATGTTAC GTGCAAAGGT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3001      GATTGTCACC CCCCGAAAGA CCATATTGTG ACACACCCTC AGTATCACGC CCAAACATTT 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3061      ACAGCCGCGG TGTCAAAAAC CGCGTGGACG TGGTTAACAT CCCTGCTGGG AGGATCAGCC 
               
               
                                               VEE GLY 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3121      GTAATTATTA TAATTGGCTT GGTGCTGGCT ACTATTGTGG CCATGTACGT GCTGACCAAC 
               
               
                            VEE GLY                              3′UTR 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~        ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3181      CAGAAACATA ATTAATAGTA AGCGGCCGCA TACAGCAGCA ATTGGCAAGC TGCTTACATA 
               
               
                                                3′UTR 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3241      GAACTCGCGG CGATTGGCAT GCCGCCTTAA AATTTTTATT TTATTTTTCT TTTCTTTTCC 
               
               
                               3′UTR 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3301      GAATCGGATT TTGTTTTTAA TATTTCAAAA AAAAAAAAAA AAAA AAAAAAAAAA 
               
               
                                             HDV ribozyme 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3361      AGGGTCGGCA TGGCATCTCC ACCTCCTCGC GGTCCGACCT GGGCATCCGA AGGAGGACGC 
               
               
                              HDV ribozyme 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3421      ACGTCCACTC GGATGGCTAA GGGAGAGCCA CGTTTAAACA CGTGATATCT GGCCTCATGG 
               
               
                  3481      GCCTTCCTTT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAACAT 
               
               
                  3541      GGTCATAGCT GTTTCCTTGC GTATTGGGCG CTCTCCGCTT CCTCGCTCAC TGACTCGCTG 
               
               
                                                                     colE1 
               
               
                                                           ~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3601      CGCTCGGTCG TTCGGGTAAA GCCTGGGGTG CCTAATGAGC AAAAGGCCAG CAAAAGGCCA 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3661      GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3721      ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3781      AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3841      GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3901      GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  3961      TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4021      ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4081      GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4141      TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4201      CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
               
               
                                                colE1 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                  4261      GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
               
               
                  4321      GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
               
               
                  4381      AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
               
               
                  4441      GGTCTGACAG TTATTAGAAA AATTCATCCA GCAGACGATA AAACGCAATA CGCTGGCTAT 
               
               
                                ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                       KanR 
               
               
                  4501      CCGGTGCCGC AATGCCATAC AGCACCAGAA AACGATCCGC CCATTCGCCG CCCAGTTCTT 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  4561      CCGCAATATC ACGGGTGGCC AGCGCAATAT CCTGATAACG ATCCGCCACG CCCAGACGGC 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  4621      CGCAATCAAT AAAGCCGCTA AAACGGCCAT TTTCCACCAT AATGTTCGGC AGGCACGCAT 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  4681      CACCATGGGT CACCACCAGA TCTTCGCCAT CCGGCATGCT CGCTTTCAGA CGCGCAAACA 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  4741      GCTCTGCCGG TGCCAGGCCC TGATGTTCTT CATCCAGATC ATCCTGATCC ACCAGGCCCG 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  4801      CTTCCATACG GGTACGCGCA CGTTCAATAC GATGTTTCGC CTGATGATCA AACGGACAGG 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  4861      TCGCCGGGTC CAGGGTATGC AGACGACGCA TGGCATCCGC CATAATGCTC ACTTTTTCTG 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  4921      CCGGCGCCAG ATGGCTAGAC AGCAGATCCT GACCCGGCAC TTCGCCCAGC AGCAGCCAAT 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  4981      CACGGCCCGC TTCGGTCACC ACATCCAGCA CCGCCGCACA CGGAACACCG GTGGTGGCCA 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  5041      GCCAGCTCAG ACGCGCCGCT TCATCCTGCA GCTCGTTCAG CGCACCGCTC AGATCGGTTT 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  5101      TCACAAACAG CACCGGACGA CCCTGCGCGC TCAGACGAAA CACCGCCGCA TCAGAGCAGC 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  5161      CAATGGTCTG CTGCGCCCAA TCATAGCCAA ACAGACGTTC CACCCACGCT GCCGGGCTAC 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                                 KanR 
               
               
                  5221      CCGCATGCAG GCCATCCTGT TCAATCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
               
               
                     ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
               
               
                                 KanR 
               
               
                  5281      ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
               
               
                  5341      TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTAA ATTGTAAGCG TTAATATTTT 
               
               
                  5401      GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT 
               
               
                  5461      CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG GCCGCTACAG 
               
               
                  5521      GGCGCTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGTTTC GGTGCGGGCC 
               
               
                  5581      TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA 
               
               
                                                     T7 promoter 
               
               
                                                  ~~~~~~~~~~~~~~~~~~~~ 
               
               
                  5641      ACGCCAGGGT TTTCCCAGTC ACACGCGTAA TACGACTCAC TATAG