Patent Publication Number: US-2023133209-A1

Title: Cell classifier circuits and methods of use thereof

Description:
FIELD 
     Disclosed herein are contiguous DNA sequences encoding highly compact multi-input genetic logic gates for precise in vivo cell targeting, and methods of treating disease using a combination of in vivo delivery and such contiguous DNA sequences. 
     BACKGROUND 
     Gene therapy is on the rise as a next generation therapeutic option for genetic disease and cancer. However, current gene therapy vectors are plagued by low efficacy, high toxicity, and long developmental timelines to generate therapeutic leads. One reason for these drawbacks is insufficiently tight control of therapeutic gene expression in the gene therapy vector which leads to gene expression (i) in unintended cell types and tissues or (ii) at either insufficient or too-high dosage. In other words, precise control of gene expression, both in terms of gene product dosage (i.e., the number of protein molecules per cell) and cell type-restricted expression remains an open challenge in gene therapy. 
     SUMMARY 
     Research in biomolecular computing and synthetic biology has long sought to enable new types of therapeutic approaches based on: (i) multi-input sensing of molecular disease indicators; (ii) a molecular level computation to determine the intensity of the therapeutic response; and (iii) the potentiation of a therapy in situ in a highly precise and coordinated fashion. Described herein are cell classifier gene circuits that enable precise identification of heterogeneous cell types via complex logical integration of multiple cellular inputs. Also described herein are methods of using the classifier gene circuits to treat disease. Cancer has been considered a class of diseases that would benefit most from cell classifier approaches due to tumor similarity to healthy cells, tumor heterogeneity, and its dissemination both at primary and secondary loci. The studies described herein support the notion that multi-input gene circuits for precise cell targeting are an ideal avenue for the next generation of gene therapies. 
     As such, in some aspects the disclosure relates to contiguous polynucleic acid molecules. In some embodiments, the contiguous polynucleic acid molecule comprises: a) a first cassette encoding a first RNA whose expression is operably linked to a transactivator response element, wherein the first RNA comprises: (i) a nucleic acid sequence of an output; and (ii) a target site for a miRNA listed in TABLE 1 or a combination thereof; and b) a second cassette encoding a second RNA, wherein the second RNA comprises a nucleic acid sequence of a transactivator; wherein the transactivator of the second cassette, when expressed as a protein, binds and transactivates the transactivator response element of the first cassette. 
     In some embodiments, the first RNA comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, the first RNA comprises a 3′ UTR, and wherein the 3′ UTR comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, the first RNA comprises a 5′ UTR, and wherein the 5′ UTR comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, the second RNA further comprises a target site for a microRNA listed in TABLE 1 or a combination thereof. 
     In some embodiments, wherein the second RNA further comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, the second RNA comprises a 3′ UTR, and wherein the 3′ UTR comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, the second RNA comprises a 5′ UTR, and wherein the 5′ UTR comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, at least one miRNA target site of the first cassette and at least one miRNA target site of the second cassette are identical nucleic acid sequences or are different sequences regulated by the same miRNA. 
     In some embodiments, the first RNA and the second RNA each comprises a let-7c target site. 
     In some embodiments, the transactivator response element comprises a nucleic acid sequence listed in TABLE 3 or a combination thereof. 
     In some embodiments, expression of the second RNA is operably linked to a transcription factor response element. In some embodiments, the transcription factor response element comprises a nucleic acid sequence listed in TABLE 4 or a combination thereof. 
     In some embodiments, the transactivator binds and transactivates the transactivator response element independently. 
     In some embodiments, expression of the first RNA is operably linked to a transcription factor response element. In some embodiments, the transcription factor response element comprises a nucleic acid sequence listed in TABLE 4 or a combination thereof. 
     In some embodiments, the transactivator binds and transactivates the transactivator response element only in the presence of a transcription factor bound to the transcription factor response element. 
     In some embodiments, the first cassette and/or the second cassette comprises a promoter element. In some embodiments, the promoter element comprises a nucleic acid sequence listed in TABLE 5 or a combination thereof. In some embodiments, the promoter element comprises a mammalian promoter or promoter fragment. 
     In some embodiments: the first cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising the transactivator response element and the transcription factor response element; (ii) the nucleic acid sequence encoding the output; and (iii) a downstream component comprising a let-7c target site; and the second cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising a transcription factor response element; (ii) the nucleic acid sequence encoding the transactivator; and (iii) a downstream component comprising a let-7c target site. 
     In some embodiments, the transcription factor response element of the first cassette and the transcription factor response element of the second cassette consist of identical nucleic acid sequences. 
     In some embodiments, the transcription factor response element of the first cassette and the transcription factor response element of the second cassette consist of different nucleic acid sequences. 
     In some embodiments, the first cassette and/or the second cassette comprises two or more transcription factor response elements. 
     In some embodiments, the first cassette and/or the second cassette comprises two different transcription factor response elements. 
     In some embodiments, the upstream regulatory component of the first cassette comprises a promoter element. In some embodiments, the promoter element comprises a mammalian promoter or promoter fragment. 
     In some embodiments, the upstream regulatory component of the second cassette comprises a promoter element. In some embodiments, the promoter element comprises a mammalian promoter or promoter fragment. 
     In some embodiments, the first cassette and the second cassette are in a convergent orientation. In some embodiments, first cassette and the second cassette are in a divergent orientation. In some embodiments, the first cassette and the second cassette are in a head-to-tail orientation. 
     In some embodiments, the first cassette and/or the second cassette is flanked by an insulator. 
     In some embodiments, the transactivator of the second cassette is tTA, rtTA, PIT-RelA, PIT-VP16, ET-VP16, ET-RelA, NarLc-VP16, or NarLc-RelA. 
     In some embodiments, the transactivator of the second cassette comprises a nucleic acid sequence listed in TABLE 2. 
     In some embodiments, the output is a protein or an RNA molecule. In some embodiments, the output is a therapeutic. In some embodiments, the output is a fluorescent protein, a cytotoxin, an enzyme catalyzing a prodrug activation, an immunomodulatory protein and/or RNA, a DNA-modifying factor, cell-surface receptor, a gene expression-regulating factor, a kinase, an epigenetic modifier, and/or a factor necessary for vector replication, and/or a sequence encoding an antigen polypeptide of a pathogen. In some embodiments, the output is the thymidine kinase enzyme from human simplex herpes virus 1 (HSV-TK). In some embodiments, the immunomodulatory protein and/or RNA is a cytokine or a colony stimulating factor. In some embodiments, the DNA-modifying factor is a gene encoding a protein intended to correct a genetic defect, a DNA-modifying enzyme, and/or a component of a DNA-modifying system. In some embodiments, the DNA-modifying enzyme is a site-specific recombinase, homing endonuclease, or a protein component of a CRISPR/Cas DNA modification system. In some embodiments, the gene expression-regulating factor is a protein capable of regulating gene expression or a component of a multi-component system capable of regulating gene expression. 
     In some embodiments, the contiguous polynucleic acid molecule comprising a nucleic acid sequence listed in TABLE 6. 
     In some embodiments, the contiguous polynucleic acid molecule comprises a cassette encoding an RNA whose expression is operably linked to a transactivator response element, wherein the RNA comprises: (i) a nucleic acid sequence of an output; (ii) a nucleic acid sequence of a transactivator; and (iii) a target site for a miRNA listed in TABLE 1 or a combination thereof; wherein the transactivator, when expressed as a protein, binds and transactivates the transactivator response element. 
     In some embodiments, the first RNA comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, the RNA further comprises a nucleic acid sequence of a polycistronic expression element separating the nucleic acid sequences of the output and the transactivator. 
     In some embodiments, the RNA comprises a 3′ UTR, and wherein the 3′ UTR comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, the RNA comprises a 5′UTR, and wherein the 5′ UTR comprises a let-7c target site, a let-7a target site, a let-7b target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof. 
     In some embodiments, the RNA comprises a let-7c target site. 
     In some embodiments, the transactivator response element comprises a nucleic acid sequence listed in TABLE 3 or a combination thereof. 
     In some embodiments, the transactivator binds and transactivates the transactivator response element independently. 
     In some embodiments, the expression of the RNA is operably linked to a transactivator response element and a transcription factor response element. In some embodiments, the transcription factor response element comprises a nucleic acid sequence listed in TABLE 4 or a combination thereof. 
     In some embodiments, the transactivator binds and transactivates the transactivator response element only in the presence of a transcription factor bound to the transcription factor response element. 
     In some embodiments, the cassette comprises a promoter element. In some embodiments, the promoter element comprises a nucleic acid sequence listed in TABLE 5 or a combination thereof. In some embodiments, the promoter element comprises a mammalian promoter or promoter fragment. 
     In some embodiments, the contiguous polynucleic acid molecule comprises, from 5′ to 3′: (i) an upstream regulatory component comprising the transactivator response element and the transcription factor response element; (ii) the nucleic acid sequence encoding the output and the transactivator; and (iii) a downstream component comprising a let-7c target site. 
     In some embodiments, the upstream regulatory component in (i) comprises a promoter element. In some embodiments, the promoter element comprises a mammalian promoter or promoter fragment. 
     In some embodiments, the transactivator of at least one cassette is tTA, rtTA, PIT-RelA, PIT-VP16, ET-VP16, ET-RelA, NarLc-VP16, or NarLc-RelA. 
     In some embodiments, the output is a protein or an RNA molecule. In some embodiments, the output is a therapeutic protein or RNA molecule. In some embodiments, the output is a fluorescent protein, a cytotoxin, an enzyme catalyzing a prodrug activation, an immunomodulatory protein and/or RNA, a DNA-modifying factor, cell-surface receptor, a gene expression-regulating factor, a kinase, an epigenetic modifier, and/or a factor necessary for vector replication, and/or a sequence encoding an antigen polypeptide of a pathogen. In some embodiments, the output is the thymidine kinase enzyme from human simplex herpes virus 1 (HSV-TK). In some embodiments, the immunomodulatory protein and/or RNA is a cytokine or a colony stimulating factor. In some embodiments, the DNA-modifying factor is a gene encoding a protein intended to correct a genetic defect, a DNA-modifying enzyme, and/or a component of a DNA-modifying system. In some embodiments, the DNA-modifying enzyme is a site-specific recombinase, homing endonuclease, or a protein component of the CRISPR/Cas system. In some embodiments, the gene expression-regulating factor is a protein capable of regulating gene expression or a component of a multi-component system capable of regulating gene expression. 
     In other aspects, the disclosure relates to vectors comprising a contiguous polynucleic acid described herein. 
     In other aspects, the disclosure relates to engineered viral genomes comprising a contiguous polynucleic acid described herein. In some embodiments, the engineered viral genome is derived from an adeno-associated virus (AAV) genome, a lentivirus genome, an adenovirus genome, a herpes simplex virus (HSV) genome, a Vaccinia virus genome, a poxvirus genome, a Newcastle Disease virus (NDV) genome, a Coxsackievirus genome, a rheovirus genome, a measles virus genome, a Vesicular Stomatitis virus (VSV) genome, a Parvovirus genome, a Seneca valley viral genome, a Maraba virus genome or a common cold virus genome. 
     In other aspects, the disclosure relates to virions comprising an engineered viral genome disclosed herein. In some embodiments, the virion comprises an AAV-DJ, AAV8, AAV6, or AAV-B1 capsid. 
     In other aspects, the disclosure relates to methods of stimulating a cell-specific event in a population of cells. In some embodiments, a method of stimulating a cell-specific event in a population of cells comprises contacting a population of cells with a contiguous polynucleic acid molecule described herein, a vector described herein, an engineered viral genome described herein, or a virion described herein, wherein the population of cells comprises at least one target cell type and one or more non-target cell types, wherein the target cell type(s) and the non-target cell types differ in levels and/or activity of one or more endogenous miRNAs, such that the levels and/or activity of the one or more endogenous miRNAs are at least two times higher in each of the two or more non-target cells relative to each of the target cells; and wherein the cell-specific event is regulated by expression levels of the output in the cells of the population of cells. 
     In some embodiments, at least a subset of the target cells and at least a subset of the non-target cells differ in levels or activity of an endogenous transcription factor, wherein the contiguous nucleic acid molecule further comprises a transcription factor response element corresponding to the endogenous transcription factor. 
     In some embodiments, at least a subset of the target cells and at least a subset of the non-target cells differ in levels or activity of a promoter fragment, wherein the contiguous nucleic acid molecule further comprises this promoter fragment. 
     In other aspects, the disclosure relates to methods of diagnosing a disease or condition. In some embodiments, a method of diagnosing a disease or a condition comprising administering a contiguous polynucleic acid molecule described herein, a vector described herein, an engineered viral genome described herein, or a virion described herein to a subject exhibiting one or more signs or symptoms associated with a disease or condition, wherein the levels of the output indicates the presence or absence of the disease and or condition. 
     In some embodiments, the disease is cancer. In some embodiments, the cancer is hepatocellular carcinoma (HCC), metastatic colorectal cancer, a metastatic tumor in the liver, breast cancer, lung cancer, retinoblastoma, and glioblastoma. 
     In other aspects, the disclosure relates to methods of treating a disease or a condition. In some embodiments, a method of treating a disease or a condition comprising administering a contiguous polynucleic acid molecule described herein, a vector described herein, an engineered viral genome described herein, or a virion described herein to a subject having the disease or condition. 
     In some embodiments, the method further comprises administering a prodrug, optionally wherein the prodrug is ganciclovir, optionally wherein the contiguous polynucleic acid molecule comprises a nucleic acid sequence listed in TABLE 6. 
     In some embodiments, the disease is cancer. In some embodiments, the cancer is hepatocellular carcinoma (HCC), metastatic colorectal cancer, a metastatic tumor in the liver, breast cancer, lung cancer, retinoblastoma, and glioblastoma. 
     In some aspects, the disclosure relates to method for use in a method of stimulating a cell-specific event. In some embodiments, a composition for use in a method of stimulating a cell-specific event in a population of cells comprises contacting a population of cells with a contiguous polynucleic acid molecule described herein, a vector described herein, an engineered viral genome described herein, or a virion described herein, wherein the population of cells comprises at least one target cell type and one or more non-target cell types, wherein the target cell type(s) and the non-target cell types differ in levels and/or activity of one or more endogenous miRNAs, such that the levels and/or activity of the one or more endogenous miRNAs are at least two times higher in each of the two or more non-target cells relative to each of the target cells; and wherein the cell-specific event is regulated by expression levels of the output in the cells of the population of cells. 
     In some embodiments, at least a subset of the target cells and at least a subset of the non-target cells differ in levels or activity of an endogenous transcription factor, wherein the contiguous nucleic acid molecule further comprises a transcription factor response element corresponding to the endogenous transcription factor. 
     In some embodiments, at least a subset of the target cells and at least a subset of the non-target cells differ in levels or activity of a promoter fragment, wherein the contiguous nucleic acid molecule further comprises this promoter fragment. 
     In other aspects, the disclosure relates to compositions for use in a method of diagnosing a disease or condition. In some embodiments, a composition for use in a method of diagnosing a disease or a condition comprises administering a contiguous polynucleic acid molecule described herein, a vector described herein, an engineered viral genome described herein, or a virion described herein to a subject exhibiting one or more signs or symptoms associated with a disease or condition, wherein the levels of the output indicates the presence or absence of the disease and or condition. 
     In some embodiments, the disease is cancer. In some embodiments, the cancer is hepatocellular carcinoma (HCC), metastatic colorectal cancer, a metastatic tumor in the liver, breast cancer, lung cancer, retinoblastoma, and glioblastoma. 
     In other aspects, the disclosure relates to compositions for use in a method of treating a disease or condition. In some embodiments, composition for use in a method of treating a disease or a condition comprising administering a contiguous polynucleic acid molecule described herein, a vector described herein, an engineered viral genome described herein, or a virion described herein to a subject having the disease or condition. 
     In some embodiments, the method further comprises administering a prodrug, optionally wherein the prodrug is ganciclovir, optionally wherein the contiguous polynucleic acid molecule comprises a nucleic acid sequence listed in TABLE 6. 
     In some embodiments, the disease is cancer. In some embodiments, the cancer is hepatocellular carcinoma (HCC), metastatic colorectal cancer, a metastatic tumor in the liver, breast cancer, lung cancer, retinoblastoma, and glioblastoma. 
     In other aspects, the disclosure relates to methods of stimulating a cell-specific event in a population of cells. In some embodiments, a method of stimulating a cell-specific event in a population of cells comprises contacting the population of cells with the contiguous polynucleic acid molecule or a composition comprising said contiguous polynucleic aid molecule, wherein: a) the population of cells comprises at least one target cell type and two or more non-target cell types, wherein the target cell type(s) and the non-target cell types differ in levels of one or more endogenous miRNAs, such that the levels of the one or more endogenous miRNAs are at least two times higher in at least a subset of the non-target cells, such as at least two and optionally each of the two or more non-target cells, relative to each of the target cells; and b) the contiguous polynucleic acid molecule comprises: (i) a first cassette encoding a RNA whose expression is operably linked to a transactivator response element, wherein the first RNA comprises: a nucleic acid sequence of an output; and one or more miRNA target sites corresponding to the one or more endogenous miRNAs; and (ii) a second cassette encoding a second RNA, wherein the second RNA comprises a nucleic acid sequence of a transactivator; wherein the transactivator of the second cassette, when expressed as a protein, binds and transactivates the transactivator response element of the first cassette; and wherein the cell-specific event is regulated by expression levels of the output in the cells of the population of cells. In some embodiments, the contiguous polynucleic acid molecule comprises a nucleic acid sequence listed in TABLE 6. 
     In some embodiments, a method of stimulating a cell-specific event in a population of cells comprising contacting the population of cells with the contiguous polynucleic acid molecule or a composition comprising said contiguous polynucleic aid molecule, wherein: a) the population of cells comprises at least one target cell type and two or more non-target cell types, wherein the target cell type(s) and the non-target cell types differ in levels of one or more endogenous miRNAs, such that the levels of the one or more endogenous miRNAs are at least two times higher in at least a subset of the non-target cells, such as at least two and optionally each of the two or more non-target cells, relative to each of the target cells; and b) the contiguous polynucleic acid molecule comprises a cassette encoding a mRNA whose expression is operably linked to a transactivator response element, wherein the RNA comprises: a nucleic acid sequence of an output; a nucleic acid sequence of a transactivator; and one or more miRNA target sites corresponding to the one or more endogenous miRNAs; and wherein the transactivator, when expressed as a protein, binds and transactivates the transactivator response element of the cassette; and wherein the cell-specific event is regulated by expression levels of the output in the cells of the population of cells. 
     In some embodiments, a composition comprising the contiguous polynucleic aid molecule comprises a vector comprising the contiguous polynucleic acid, an engineered viral genome comprising the contiguous polynucleic acid, or a virion comprising the polynucleic acid. 
     In some embodiments, the endogenous miRNA is selected from the miRNAs listed in TABLE 1 or a combination of miRNAs listed in TABLE 1. In some embodiments, the endogenous miRNA is selected from the group consisting of let-7c, let-7a, let-7b, let-7d, let-7e, let-7f, let-7g, let-7i, miR-22, miR-26b, miR-122, miR-208a, miR-208b, miR-1, miR-217, miR-216a, or a combination thereof. 
     In some embodiments, at least a subset of the target cells and at least a subset of the non-target cells differ in levels or activity of an endogenous transcription factor, wherein the contiguous nucleic acid molecule further comprises a transcription factor response element corresponding to the endogenous transcription factor. 
     In some embodiments, at least a subset of the target cells and at least a subset of the non-target cells differ in levels or activity of a promoter fragment, wherein the contiguous nucleic acid molecule further comprises this promoter fragment. 
     In some embodiments, the target cells are tumor cells and the cell-specific event is tumor cell death. In some embodiments, the tumor cell death is mediated by immune targeting through the expression of activating receptor ligands, specific antigens, stimulating cytokines or any combination thereof. 
     In some embodiments, the target cells are senescent cells and the cell-specific event is senescent cell death. 
     In some embodiments, the method further comprises contacting the population of cells with prodrug or a non-toxic precursor compound that is metabolized by the output into a therapeutic or a toxic compound. 
     In some embodiments, output expression ensures the survival of the target cell population while the non-target cells are eliminated due to lack of output expression and in the presence of an unrelated and unspecific cell death-inducing agent. 
     In some embodiments, the target cells comprise a particular phenotype of interest such that output expression is limited to the cells of this particular phenotype. 
     In some embodiments, the target cells are a cell type of choice and the cell-specific event is the encoding of a novel function, through the expression of a gene naturally absent or inactive in the cell type of choice. 
     In some embodiments, the population of cells comprises a multicellular organism. In some embodiments, the multicellular organism is an animal. In some embodiments, the animal is a human. 
     In some embodiments, the population of cells is contacted ex-vivo. In some embodiments, the population of cells is contacted in-vivo. 
     In other aspects, the disclosure relates to contiguous polynucleic acid molecules. In some embodiments, a contiguous polynucleic acid molecule comprises: a) a first cassette encoding a first RNA whose expression is operably linked to a transactivator response element, wherein the first RNA comprises: (i) a nucleic acid sequence of an output; and (ii) a target site for a miRNA, wherein said miRNA is highly expressed and/or active in at least two different healthy tissues of a mammal and is expressed at low level in one or more types of target cells; b) a second cassette encoding a second RNA, wherein the second RNA comprises a nucleic acid sequence of a wherein the transactivator of the second cassette, when expressed as a protein, binds and transactivates the transactivator response element of the first cassette. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The following drawings form part of the present specification and are included to further demonstrate certain aspects of the present disclosure, which can be better understood by reference to one or more of these drawings in combination with the detailed description of specific embodiments presented herein. It is to be understood that the data illustrated in the drawings in no way limit the scope of the disclosure. 
         FIGS.  1 A- 1 N . Translation of a multi-plasmid circuit architecture to a viral vector.  FIG.  1 A . Schematics of genetic arrangements. Divergent (top) and convergent (bottom) arrangements were made; two variants were made for each, using different variants of the auxiliary transactivator PIT (divergent: D-P2: PIT=PIT::RelA; D-PV: PIT=PIT::VPI6; convergent: C-P2: PIT=PIT::RelA; C-PV: PIT=PIT::VPI6).  FIG.  1 B . Testing of backbone DNA performance using transient transfections and ectopic input expression in HeLa cells. Bars in each grouping, from left to right: C-P2, D-P2, C-PV, D-PV.  FIG.  1 C . Evaluation of constructs&#39; response to endogenous inputs in HuH-7 and HeLa cells. Bars in each grouping, from left to right: C-P2, D-P2, C-PV, D-PV.  FIG.  1 D . Schematics of constructs incorporating miRNA targets as robust Off switches, illustrated using the miR-424 target sequence. Divergent (top) and convergent (bottom) arrangements were made; two variants were made for each, using different variants of the auxiliary transactivator PIT (divergent: D-P2: PIT=PIT::RelA-T424; D-PV: PIT=PIT::VPI6-T424; convergent: C-P2: PIT=PIT::RelA-T424; C-PV: PIT=PIT::VPI6-T424).  FIG.  1 E . Validation of the AND-gate component of the logic program in HeLa cells via ectopic expression of TF inputs. Bars in each grouping, from left to right: C-P2-T424, D-P2-T424, C-PV-T424, D-PV-T424.  FIG.  1 F . Evaluation of circuit response to endogenous transcriptional inputs in HuH-7 and HeLa cells. The order of bars is identical to  FIG.  1 E .  FIG.  1 G . A complete evaluation of the three-input program encoded on the divergent orientation in HeLa cells using ectopic input delivery. The input combination with only miR-424 present was not evaluated due to obvious futility, given the lack of expression in the absence of all inputs and the fact that miR-424 is a negative regulator. Bars in each grouping, from left to right: D-P2-T424, D-PV-T424.  FIG.  1 H . Functionality of the miRNA switch in the presence of inducing TF inputs. Circuit output is tested in HuH-7 cells with and without ectopic transfection of miR-424 mimic (indicated under X axis). The order of bars is identical to  FIG.  1 G .  FIG.  1 I . Evaluation of circuits harboring miR-126 target with respect to their repressibility in the presence of endogenously expressed inducing TF inputs. The order of bars is identical to  FIG.  1 G .  FIG.  1 J . Evaluation of the miRNA target effect on cell classification performance with two HCC cells lines and HeLa cells as a negative control. Bars in each grouping, from left to right: D-P2, D-PV, D-P2-T424, D-PV-T424, C-PV-T126, D-PV-T126.  FIG.  1 K . Evaluation of the circuit panel, with and without miRNA sensors incorporated, packaged into DJ-pseudotyped AAV vectors, in HCC cell lines HepG2 and HuH-7. HeLa and HCT-116 cell lines are used as counter samples. Bars in each grouping, from left to right: CMV, D-P2, D-PV, D-P2-T424, D-PV-T424, C-PV-T126, D-PV-T126.  FIG.  1 L . In vitro evaluation of a panel of miRNAs for their capacity to distinguish healthy primary hepatocytes from HCC cell lines. Bars in each grouping, from left to right: TFF5, T424, T126, T122.  FIGS.  1 M- 1 N . The exploration of different miRNA target arrangements and their impact on the magnitude of output repression.  FIG.  1 M . Schematics of the different constructs and their shorthand notations.  FIG.  1 N . Output generated in the HepG2 cells (no miR-122 expression) and HuH-7 cells (intermediate level of miR-122 expression). Bars in each grouping, from left to right: HepG2, Huh-7. Abbreviations: ITR: internal terminal repeat of AAV2; pA: an SV40 polyadenylation signal (convergent orientation), hGH next to mCherry and SV40 pA next to PIT genes in divergent orientation; Cherry: a sequence encoding an mCherry fluorescent protein; TATA: a minimal TATA box (Angelici et al., 2016); HNF1 RE: a response element binding HNF1A and HNF1B; PIT RE: a response element binding PIT::RelA and PIT::VP16 transactivator; SOX RE: a DNA sequence that binds SOX9 and SOX10 transcription factors, and possibly other transcription factors from the SOX family SOX1-SOX15, SOX17, SOX18, SOX21, SOX30, and SRY; PIT: pristinomycin-inducible transactivator (Fussenegger et al., 2000), which stands either for PIT:RelA or PIT::VP16 fusion. Chart designs: The normalized expression of the output mCherry is shown on Y axis. 
         FIGS.  2 A- 2 F . Pilot evaluation of specificity and efficacy in the orthotopic mouse model of HCC.  FIG.  2 A . In vitro validation of cell classification capacity of the chosen circuit packaged into DJ-pseudotyped viral vector.  FIG.  2 B . In vitro cell elimination by the circuit with HSV-TK output, compared to the constitutive control vector. Schematics of the circuits employed here are shown above the bar charts. For every cell line or primary hepatocytes, the dose-response to ganciclovir (X axis) is measured in the presence of a constitutive HSV-TK vector, the circuit, and with GCV alone. Cell viability MTS readouts are shown on Y axis.  FIG.  2 C . The progression of tumor load in tumor-bearing mice, shown for different experimental arms of the pilot experiment (n=2), as indicated in the panel.  FIG.  2 D . Tumor load in the liver at termination, quantified by luminescence, the images on the left are superpositions of livers (grayscale) and the bioluminescent signal.  FIG.  2 E . Quantitative analysis of the tumor load in the livers post-termination.  FIG.  2 F . The correlation between tumor load soon after inoculation, and the tumor load at termination. The two mice from the treatment arm are represented by two red dots. 
         FIGS.  3 A- 3 F . Identification of a selective and broadly-applicable miRNA input for the tumor-targeting program.  FIG.  3 A . The schematics of cell profiling and ranking of miRNA candidates based on their high expression in healthy liver and low expression in the HCC samples.  FIG.  3 B . The schematics of functional validation of the pre-selected miRNA inputs. A reporter viral vector is created for every input, and every vector is delivered to every sample of interest (one by one) to evaluate the biological activity of the inputs.  FIG.  3 C . The results of the functional evaluation of a miRNA panel in two HCC cell lines and primary healthy hepatocytes. Low reporter expression corresponds to high miRNA activity. FF5 is a control target.  FIG.  3 D . The correlation between the miRNA expression count identified in the NGS profiling experiment (Dastor et al., 2018) and the functional response of selected miRNA sensors. The trend line is fit to a repressor Hill function.  FIG.  3 E . The quantified expression of a panel of miRNA reporter vectors in different mouse organs, following systemic delivery. Expression of different reporters in the same organ (indicated above a chart) is grouped together. The bar shading indicates in which organ the reporter was expected to respond based on literature analysis and profiling data. The values are normalized to the control vector bearing TFF5 target; with that, it is clear that this target is responding to cryptic inputs in vivo and many reporters result in output values above 1.  FIG.  3 F . Representative images of reporter expression in various organs. The name of the reporter is indicated on the left. The cerulean panel shows the expression of constitutive mCerulean internal control. The Cherry panel shows the residual expression of the mCherry reporter, furnished with the indicated miRNA target. 
         FIGS.  4 A- 4 C . Validation of circuit specificity in vitro.  FIG.  4 A . The panel of control constructs used to evaluate a circuit&#39;s mechanism of action. The abbreviations are the same as in  FIGS.  1 A,  1 D and  1 M .  FIG.  4 B . Mapping C.TF-AND sub-circuit response to endogenous inputs in 10 cell lines and primary hepatocytes. For every cell line, the log-transformed output of the feedback-amplified sensor for SOX9/10 and HNF1A/B, normalized to the constitutive output in these cells, is shown respectively on X and Y axis. The output of the C.TF-AND circuit is shown on Z axis.  FIG.  4 C . Mapping HCC.V2 circuit response in 10 cell lines and primary hepatocytes. Log-transformed output of the C.TF-AND circuit and log-transformed C.let-7c reporter circuit response magnitude are plotted on axes X and Y, while the output of the complete circuit in every cell line is shown on axis Z. All values for a given cell type are normalized to constitutive expression in that cell type. 
         FIGS.  5 A- 5 D . In vivo characterization of circuit targeting specificity.  FIG.  5 A . Output of selected sub-programs, control vector, the full program, and background, obtained using B1-pseudotyped AAV vectors in various organs. The values are obtained by quantitative image analysis.  FIG.  5 B . Images of tissue slices representing different organs, showing the expression of mCherry from different vectors as indicated. The Phase image and the mCherry channel are shown. Two different exposures are used to represent pancreas slices, to reflect the large dynamic range of the mCherry change.  FIG.  5 C . Expression of mCherry output from HCC.V2 circuit in the tumor and in the organs of HepG2-tumor bearing mice. The tumor is stably transduced with mCitrine and is showing in the Yellow fluorescent channel.  FIG.  5 D . Quantitative analysis of mCherry expression in the tumor and various organs of tumor-bearing mice, obtained using image processing. 
         FIGS.  6 A- 6 B . In vitro efficacy of the circuit and controls in two HCC cell lines and primary hepatocytes.  FIG.  6 A . Dose-response to GCV in the absence of any AAV vector (squares), in the presence of a constitutive HSV-TK expression cassette (triangles) or the complete circuit (circles). Cell viability measured using MTS assay is shown on Y axis. Schematic representations of the circuits and their IDs are shown on top.  FIG.  6 B . The sensitivity of HuH-7 cell line to different vector dosage of the constitutive HSV-TK cassette and the two different tumor targeting programs. Top chart, comparison between the two circuit variants; bottom, the comparison between the constitutive vector and the second circuit variant. 
         FIGS.  7 A- 7 F . Efficacy of HCC-targeting circuit in orthotopic mouse model.  FIG.  7 A . The schematics of tumor establishment and treatment regimen.  FIG.  7 B . Tumor load over time in various experimental arms. Tumor load, measured via in vivo whole-body bioluminescence, is imaged over time. For each animal, the load is normalized to the load on the day before initiating GCV injection regimen.  FIG.  7 C . A spider plot showing tumor load development for individual animals in the main experimental arms, normalized to the tumor load on the day before initiating GCV injection regimen.  FIG.  7 D . Representative images of whole-body luminescence of individual animals from a number of experimental arms.  FIG.  7 E . Images of individual livers and the tumor load in the liver measured by whole-organ bioluminescence at termination for a number of experimental arms.  FIG.  7 F . Quantification of the tumor load in  FIG.  7 E . 
         FIGS.  8 A- 8 C . In vivo evaluation of AAV-B1 tumor transduction.  FIG.  8 A . Output of control vector, C.TF-AND subprogram and the full program packaged in DJ-pseudotyped AAV vectors are compared to the output of the full circuit packaged in B1-pseudotuped AAV vectors in liver and HepG2-tumors. The tumor is stably transduced with mCitrine and is showing in the Yellow fluorescent channel.  FIG.  8 B . Quantification of HCC.V2 driven output level (mCherry) in the tumor upon AAV-DJ and AAV-B1 delivery. The values are obtained by quantitative image analysis.  FIG.  8 C . Output from HCC.V2 circuit delivered by B1-pseudotyped AAV in core section of a large tumor nodule. 
         FIGS.  9 A- 9 B . Rational design of optimized circuit combining multiple liver protective miRNAs.  FIG.  9 A . Schematics of candidate circuits (HCC.V3) that combine strong miR-let7c and weak miR-122 repression. The strong miR-let7c repression is obtained by using the target configuration describe in HCC.V2. The repression strength elicited by miR-122 can be tuned by varying the number, arrangement or sequence of the miRNA targets. Depicted are shown 3 different strategies to reduce miR-122 repression levels compared to HCC.V1: (i) use of a perfect miR-122 target (T-122*) only on the transactivator branch of the circuit; (ii) double repression of the transactivator and the output using miR-122 targets with imperfect complementarity (T-122*); or (iii) a mixed approach that relies on perfect target to repress the transactivator and imperfect miRNA targets to repress the output. The candidate that maximizes the repression in liver lines while minimizing the loss of expression in a panel of HCC cell lines (HUH-7 in particular) is selected. Each candidate is tested in both possible miRNA targets relative positioning variants.  FIG.  9 B . Example of imperfect miR-122 target (T-122*) derived from the conserved UTR region of an endogenous gene (P4HA1) regulated by miR-122 (SEQ ID NOS: 305 and 306, top and bottom respectively). Targets with imperfect complementarity are obtained either by using sequence occurring in endogenous genes or by introducing random mutations in the region flanking the miRNA seed sequence. Both approaches will be used to create a selection of targets with different dose-response profiles. 
     
    
    
     DETAILED DESCRIPTION 
     One of the promises of molecular computing (Benenson, 2012) and synthetic biology (Weber and Fussenegger, 2012) has been the rational design of “smart” therapies (Benenson et al., 2004) that sense and respond to disease-related cues in complex fashion and in real time, resulting in precise and “on demand” therapeutic actuation. In order to deliver on this promise, three separate challenges are addressed. First, a disease mechanism is sufficiently understood in order to design blueprints for a therapeutically relevant sense-compute-respond cascades. In particular, relevant inputs are identified and the program that would result in the most efficacious and the least toxic response preferably is determined. Second, robust synthetic biology platforms capable of implementing these therapeutic cascades exist, or are developed de novo for the purpose. Third, these platforms are adapted to clinically-relevant therapeutic modalities. Among the latter, cell and gene therapies have been identified as the most suitable for the clinical translation of synthetic gene circuits, given the fact that both of these modalities enable, and often require, the incorporation of engineered genetic payload. 
     Addressing all three challenges narrows down the field of potential medical indications to develop the approach in the translational setting. One line of work has focused on cell-based implants, where the genetically modified cells are able to sense a particular disease-related cue in blood circulation and secrete a molecular agent with therapeutic properties in response. In this line of work, the cell implant serves as a sentinel and a “factory” that senses organismal disease state and produces a therapy that affects the entire organism in response (Auslander et al., 2014; Tastanova et al., 2018; Ye et al., 2017). A second line of research has built on the CAR-T cell therapy approach and augmented these cells with multi-input combinatorial sensing properties, in order to improve their specificity toward cancer cells expressing combinations of surface antigens, and reduce on-target, off-tumor effects (Cho et al., 2018; Kloss et al., 2013; Roybal et al., 2016; Zah et al., 2016). 
     Synthetic biology applications in the field of gene therapy have also shown initial success in animal disease models. A hybrid approach, combining a set of lentiviral vectors addressing ovarian cancer cells and expressing immunomodulators in these cells, and engineered T-cells, showed efficacy in a mouse model of ovarian metastasis to the peritoneal cavity. Cell targeting was implemented as a miRNA sponge-enabled AND gate between two promoters whose combination was shown to be tumor specific (Nissim et al., 2017). In another recent work, an oncolytic adenovirus was engineered to replicate based on a multi-input logical control of its life cycle and showed efficacy upon intratumoral injection into a subcutaneous tumor (Huang et al., 2019). 
     The main added value of synthetic gene circuits for gene and cell therapies arises from the sophisticated approaches to “program” the therapeutic response, that is, regulate the specificity, the timing, and the dosage of the therapeutic actuation in a predetermined fashion, potentially in a dynamic manner and in combination with various feedback regulatory motifs (Angelici et al., 2016; Xie et al., 2011). However, furnishing a known therapeutic transgene with a gene circuit regulating its expression may not necessarily be better than a more established approaches that often use a constitutively-driven or tissue-specific promoter-driven therapeutic gene packaged into a viral vector that additionally possesses a degree of organ or cell type specificity via its capsid (Al-Zaidy et al., 2019; Landegger et al., 2017; Scholl et al., 2016). Alternatively, viral vectors can be injected directly into the tissue or organ of interest (Juttner et al., 2019; Nelson et al., 2016), reducing the diversity of cell types that need to be specifically addressed. Indeed, the majority of approved therapies, including clinically approved CAR-T cells (June et al., 2018) and many gene therapies (Keeler and Flotte, 2019), engineered based on this approach, show satisfactory efficacy and safety profiles. Thus, a burden is on the synthetic biology community to prove this advantage. 
     Cancer is a disease that has tremendous potential to benefit from therapies powered by synthetic biology. Even narrowly defined cancers are heterogenous disease, both between patient groups and even between individual tumors in the same patient (Dagogo-Jack and Shaw, 2018). Tumors in a patient are often spread between primary and metastatic loci, making intratumoral injection possible only for a subset of cases. Lastly, anti-tumor therapies are very toxic, meaning that their activation in non-tumor cells will lead to often dramatic adverse effects. Together, the requirement to address a complex, heterogeneous cell population precisely, combined with the need to deliver the agent systemically to address a spread population of tumors, suggests that the use of synthetic biology approaches can be beneficial. 
     Disclosed herein are contiguous polynucleic acid molecules that encode classifier gene circuits compatible with commonly used gene therapy viral and non-viral vectors. Also disclosed herein are methods of implementing complex multi-input control over the expression of an output (i.e., gene of interest) in a population of cells. These methods include gene therapies for the diagnosis and treatment of diseases such as cancer (e.g., hepatocellular carcinoma (HCC)). 
     I. Compositions of Contiguous Polynucleic Acid Molecules 
     In some aspects, the disclosure relates to contiguous polynucleic acid molecules comprising a gene circuit. As used herein, the term “contiguous polynucleic acid molecule” refers to a single, continuous nucleic acid molecule (i.e., a single-stranded polynucleic acid molecule) or two complementary continuous nucleic acid molecules (i.e., a double-stranded polynucleic acid molecule comprising two complementary strands). In some embodiments, the contiguous polynucleic acid is an RNA (e.g., single-stranded or double-stranded). In some embodiments, the contiguous polynucleic acid is a DNA (e.g., single-stranded or double-stranded). In some embodiments, the contiguous polynucleic acid is a DNA:RNA hybrid. 
     A contiguous polynucleic acid described herein comprises a gene circuit that is encoded one or more expression cassettes. As used herein, the terms “expression cassette” and “cassette” are used interchangeably and refer to a polynucleic acid comprising: (i) a nucleic acid sequence encoding an RNA (e.g., comprising the nucleic acid sequence of an output and/or a transactivator); and (ii) a nucleic acid sequence that regulates expression levels of the RNA (e.g., a transactivator response element, a transcription factor response element, a minimal promoter, and/or a promoter element). 
     In some embodiments, a contiguous polynucleic acid molecule comprises a gene circuit consisting of a single cassette. In other embodiments, a contiguous polynucleic acid molecule comprises a gene circuit comprising two or more cassettes. 
     In some embodiments, a contiguous polynucleic acid molecule comprises two or more cassettes and at least two cassettes are in a divergent orientation. The term “divergent orientation,” as used herein, refers to a configuration in which: (i) transcription of a first cassette and a second cassette proceeds on different strands of the contiguous polynucleic acid molecule and (ii) transcription of the first cassette is directed away from the second cassette and transcription of the second cassette is directed away from the first cassette.  FIG.  1 A  (upper schematic) provides examples of various divergent configurations. 
     In some embodiments, a contiguous polynucleic acid molecule comprises two or more cassettes and at least two cassettes are in a convergent orientation. As used herein, the term “convergent orientation” refers to a configuration in which: (i) transcription of a first cassette and a second cassette proceeds on different strands of the contiguous polynucleic acid molecule and (ii) transcription of the first cassette is directed toward the second cassette and transcription of the second cassette is directed toward the first cassette. In some embodiments, two convergent cassettes share a polyadenylation sequence.  FIG.  1 A  (lower schematic) provides examples of various convergent configurations. 
     In some embodiments, a contiguous polynucleic acid molecule comprises two or more cassettes and at least two cassettes are in a head-to-tail orientation. As used herein, the term “head-to-tail” refers to a configuration in which: (i) transcription or translation of the first cassette and the second cassettes proceeds on the same strand of the contiguous polynucleic acid molecule and (ii) transcription or translation of the first cassette is directed toward the second cassette and transcription or translation of the second cassette is directed away from the first cassette (5′ . . . → . . . → . . . 3′). 
     In some embodiments, two cassettes are separated by one or more insulators. Insulators are nucleic acid sequences that, when bound by insulator-binding proteins, shield a regulatory component or a response component from the effects of other nearby regulatory elements. For example, flanking the cassettes of a contiguous polynucleic acid molecule can shield each cassette from the effects of regulatory elements of the other cassettes. Examples of insulators are known to those having skill in the art. 
     The gene circuits described herein utilize one or more mechanisms to regulate expression levels of an output molecule (i.e., a gene of interest). Therefore, each of the contiguous polynucleic acids described herein comprises a cassette encoding an RNA comprising the nucleic acid sequence of an output. Exemplary output molecules are provided below. The RNA comprising the nucleic acid sequence of the output is operably linked to a transactivator response element (and, optionally, one or more additional nucleic acid sequences that regulate expression of the RNA, such as a transcription factor response element, a minimal promoter, and/or a promoter element). 
     To regulate the expression levels of the output molecule (i.e., gene of interest), each of the contiguous polynucleic acids described herein further comprises: (i) a cassette encoding an RNA (e.g., mRNA) comprising the nucleic acid sequence of a transactivator; and (ii) a cassette encoding an RNA comprising a miRNA target site. Exemplary transactivators and miRNA target sites are provided below. 
     The cassette encoding the RNA (e.g., mRNA) comprising the nucleic acid sequence of the transactivator may be operably linked to a nucleic acid sequence that regulates expression of the RNA (e.g., a transactivator response element, a transcription factor response element, a minimal promoter, and/or a promoter and/or enhancer element). In some embodiments, the cassette encoding the RNA comprising the nucleic acid sequence of the transactivator is the same cassette encoding the RNA comprising the nucleic acid sequence of the output (i.e., a single RNA comprises the nucleic acid sequences of both the transactivator and the output). 
     The cassette encoding the RNA comprising the miRNA target site may be the same cassette encoding the RNA comprising the nucleic acid sequence of the output (i.e., the RNA comprising the nucleic acid sequence of the output further comprises a miRNA target site). Alternatively or in addition, the cassette encoding the RNA comprising the miRNA target site may be the same cassette encoding the RNA comprising the nucleic acid sequence of the transactivator (i.e., the nucleic acid sequence of the transactivator further comprises a miRNA target site). 
     In some embodiments, the nucleic acid sequence of an RNA encoded by a cassette further comprises a polyadenylation sequence. In some embodiments, the polyadenylation sequence is suitable for transcription termination and polyadenylation in mammalian cells. 
     (i) MiRNA Target Sites 
     Each of the contiguous polynucleic acids described herein comprise one or more cassettes encoding an RNA (e.g., the RNA comprising the nucleic sequence encoding the output and/or the RNA comprising the nucleic acid sequence of the transactivator) that comprises a miRNA target site. MiRNAs are a class of small non-coding RNAs that are typically 21-25 nucleotides in length that downregulate the levels of RNAs to which they bind in a variety of manners, including translational repression, mRNA cleavage, and deadenylation. The term “miRNA target site,” as used herein, refers to a sequence that complements and is regulated by a miRNA. A miRNA target site may have at least 25%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% complementarity to the miRNA that binds and regulates the miRNA target site. 
     In some embodiments, an RNA encoded by a cassette described herein comprises at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, or at least 20 miRNA target sites. In some embodiments, an RNA encoded by a cassette described herein comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 miRNA target sites. In some embodiments, an RNA encoded by a cassette described herein comprises 1-2, 1-3, 1-4, 1-5, 1-6, 1-7, 1-8, 1-9, 1-10, 2-3, 2-4, 2-5, 2-6, 2-7, 2-8, 2-9, 2-10, 3-4, 3-5, 3-6, 3-7, 3-8, 3-9, 3-10, 4-5, 4-6, 4-7, 4-8, 4-9, 4-10, 5-6, 5-7, 5-8, 5-9, 5-10, 6-7, 6-8, 6-9, 6-10, 7-8, 7-9, 7-10, 8-9, 8-10, or 9-10 miRNA target sites. 
     In some embodiments, an RNA encoded by a cassette described herein comprises multiple miRNA target sites and each of the miRNA target sites have identical sequences or comprise a different nucleic acid sequence that is regulated by the same miRNA. In other embodiments, an RNA encoded by a cassette described herein comprises two or more miRNA target sites that are regulated by distinct miRNAs (i.e., distinct miRNA target sites); comprising for example, at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 distinct miRNA target sites. In some embodiments, an RNA encoded by a cassette described herein comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 distinct miRNA target sites. In some embodiments, an RNA encoded by a cassette described herein comprises 1-2, 1-3, 1-4, 1-5, 1-6, 1-7, 1-8, 1-9, 1-10, 2-3, 2-4, 2-5, 2-6, 2-7, 2-8, 2-9, 2-10, 3-4, 3-5, 3-6, 3-7, 3-8, 3-9, 3-10, 4-5, 4-6, 4-7, 4-8, 4-9, 4-10, 5-6, 5-7, 5-8, 5-9, 5-10, 6-7, 6-8, 6-9, 6-10, 7-8, 7-9, 7-10, 8-9, 8-10, or 9-10 distinct miRNA target sites. 
     A miRNA target site of an RNA encoded by a cassette described herein may be located anywhere within the sequence of the RNA. For example, in some embodiments an RNA encoded by a cassette described herein comprises a 3′ UTR, and the 3′ UTR comprises a miRNA target site. In some embodiments, an RNA encoded by a cassette described herein comprises a intron, and the intron comprises a miRNA target site. In some embodiments, an RNA encoded by a cassette described herein comprises a 5′ UTR, and the 5′ UTR comprises a miRNA target site. 
     Exemplary miRNAs and miRNA target sites are listed in TABLE 1. In some embodiments, an RNA encoded by a cassette described herein comprises a miRNA target site for a miRNA listed in TABLE 1. In some embodiments, an RNA encoded by a cassette described herein comprises multiple miRNA target sites corresponding to a miRNA listed in TABLE 1 (e.g., a combination including a let-7c target site and a miR-122 target site). 
     In some embodiments, an RNA encoded by a cassette described herein comprises a miRNA target site having at least at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identity to a miRNA target site listed in TABLE 1. In some embodiments, an RNA encoded by a cassette described herein comprises multiple miRNA target sites having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identity to a miRNA target site listed in TABLE 1. 
     In some embodiments, an RNA encoded by a cassette described herein comprises a let-7a target site, a let-7b target site, a let-7c target site, a let-7d target site, a let-7e target site, a let-7f target site, a let-7g target site, a let-7i target site, a miR-22 target site, a miR-26b target site, a miR-122 target site, a miR-208a target site, a miR-208b target site, a miR-1 target site, a miR-217 target site, a miR-216a target site, or a combination thereof (e.g., a combination of a let7c target site and a miR-122 target site). 
     In some embodiments, an RNA encoded by a cassette described herein comprises a let-7c target site (i.e., a nucleic acid sequence that complements and is regulated by hsa-let-7c). In some embodiments a let-7c target site consists of the nucleic acid sequence AACCATACAACCTACTACCTCA (SEQ ID NO: 42). 
     In some embodiments, an RNA encoded by a cassette described herein comprises a miR-22 target site (i.e., a nucleic acid sequence that complements and is regulated by miR-22). In some embodiments a miR-22 target site consists of the nucleic acid sequence ACAGTTCTTCAACTGGCAGCTT (SEQ ID NO: 43). 
     In some embodiments, an RNA encoded by a cassette described herein comprises a miR-26b target site (i.e., a nucleic acid sequence that complements and is regulated by miR-26b). In some embodiments a miR-26b target site consists of the nucleic acid sequence 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 44) 
               
               
                   
                 ACCTATCCTGAATTACTTGAA. 
               
            
           
         
       
     
     In some embodiments, an RNA encoded by a cassette described herein comprises a miR-126-5p target site (i.e., a nucleic acid sequence that complements and is regulated by miR-126-5p). In some embodiments a miR-126-5p target site consists of the nucleic acid sequence CGTGTTCACAGCGGACCTTGAT (SEQ ID NO: 45). 
     In some embodiments, an RNA encoded by a cassette described herein comprises a miR-424 target site (i.e., a nucleic acid sequence that complements and is regulated by miR-424). In some embodiments a miR-424 target site consists of the nucleic acid sequence GTCCAAAACATGAATTGCTGCT (SEQ ID NO: 48). 
     In some embodiments, an RNA encoded by a cassette described herein comprises a miR-122 target site (i.e., a nucleic acid sequence that complements and is regulated by miR-122). In some embodiments a miR-122 target site consists of the nucleic acid sequence CAAACACCATTGTCACACTCCA (SEQ ID NO: 46). 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Exemplary miRNAs and exemplary miRNA target sites. 
               
            
           
           
               
               
               
               
               
               
            
               
                 SEQ 
                   
                   
                   
                 SEQ 
                   
               
               
                 ID 
                   
                   
                   
                 ID 
                   
               
               
                 NO: 
                 miRNA 
                 Accession 
                 miRNA SEQUENCE 
                 NO: 
                 TARGET SEQUENCE 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 1 
                 miR-let7c- 
                 MIMAT0000064 
                 UGAGGUAGUAGG 
                 42 
                 AACCATACAACCTACT 
               
               
                   
                 5p 
                   
                 UUGUAUGGUU 
                   
                 ACCTCA 
               
               
                   
               
               
                 2 
                 miR-22-3p 
                 MIMAT0000077 
                 AAGCUGCCAGUUG 
                 43 
                 ACAGTTCTTCAACTGGC 
               
               
                   
                   
                   
                 AAGAACUGU 
                   
                 AGCTT 
               
               
                   
               
               
                 3 
                 hsa-miR-26b 
                 MIMAT0000083 
                 UUCAAGUAAUUC 
                 44 
                 ACCTATCCTGAATTACT 
               
               
                   
                   
                   
                 AGGAUAGGU 
                   
                 TGAA 
               
               
                   
               
               
                 4 
                 hsa-miR- 
                 MIMAT0000444 
                 CAUUAUUACUUU 
                 45 
                 CGCGTACCAAAAGTAA 
               
               
                   
                 126-5p 
                   
                 UGGUACGCG 
                   
                 TAATG 
               
               
                   
               
               
                 5 
                 hsa-miR- 
                 MIMAT0000421 
                 UGGAGUGUGACA 
                 46 
                 CAAACACCATTGTCAC 
               
               
                   
                 122-5p 
                   
                 AUGGUGUUUG 
                   
                 ACTCCA 
               
               
                   
               
               
                 6 
                 Mmu-miR- 
                 MIMAT0000548 
                 CAGCAGCAAUUCA 
                 47 
                 GTCCAAAACATGAATT 
               
               
                   
                 322-5p 
                   
                 UGUUUUGGA 
                   
                 GCTGCT 
               
               
                   
               
               
                 7 
                 hsa-miR- 
                 MIMAT0001341 
                 CAGCAGCAAUUCA 
                 48 
                 GTCCAAAACATGAATT 
               
               
                   
                 424-5p 
                   
                 UGUUUUGAA 
                   
                 GCTGCT 
               
               
                   
               
               
                 8 
                 hsa-miR- 
                 MIMAT0000241 
                 AUAAGACGAGCA 
                 49 
                 ACAAGCTTTTTGCTCGT 
               
               
                   
                 208a-3p 
                   
                 AAAAGCUUGU 
                   
                 CTTAT 
               
               
                   
               
               
                 9 
                 hsa-miR- 
                 MIMAT0004960 
                 AUAAGACGAACA 
                 50 
                 ACAAACCTTTTGTTCGT 
               
               
                   
                 208b-3p 
                   
                 AAAGGUUUGU 
                   
                 CTTAT 
               
               
                   
               
               
                 10 
                 hsa-miR- 
                 MIMAT0000273 
                 UAAUCUCAGCUGG 
                 51 
                 TCACAGTTGCCAGCTG 
               
               
                   
                 216a-5p 
                   
                 CAACUGUGA 
                   
                 AGATTA 
               
               
                   
               
               
                 11 
                 mmu-miR- 
                 MIMAT0000679 
                 UACUGCAUCAGGA 
                 52 
                 TCCAGTCAGTTCCTGAT 
               
               
                   
                 217-5p 
                   
                 ACUGACUGGA 
                   
                 GCAGTA 
               
               
                   
               
               
                 12 
                 hsa-miR- 
                 MIMAT0000274 
                 UACUGCAUCAGGA 
                 53 
                 TCCAATCAGTTCCTGAT 
               
               
                   
                 217-5p 
                   
                 ACUGAUUGGA 
                   
                 GCAGTA 
               
               
                   
               
               
                 13 
                 hsa-miR- 
                 MIMAT0000728 
                 UUUGUUCGUUCG 
                 54 
                 TCACGCGAGCCGAACG 
               
               
                   
                 375-3p 
                   
                 GCUCGCGUGA 
                   
                 AACAAA 
               
               
                   
               
               
                 14 
                 hsa-miR- 
                 MIMAT0000422 
                 UAAGGCACGCGGU 
                 55 
                 TTGGCATTCACCGCGTG 
               
               
                   
                 124-3p 
                   
                 GAAUGCCAA 
                   
                 CCTTA 
               
               
                   
               
               
                 15 
                 hsa-miR-1- 
                 MIMAT0000416 
                 UGGAAUGUAAAG 
                 56 
                 ATACATACTTCTTTACA 
               
               
                   
                 3p 
                   
                 AAGUAUGUAU 
                   
                 TTCCA 
               
               
                   
               
               
                 16 
                 hsa-miR- 
                 MIMAT0000427 
                 UUUGGUCCCCUUC 
                 57 
                 CAGCTGGTTGAAGGGG 
               
               
                   
                 133a-3p 
                   
                 AACCAGCUG 
                   
                 ACCAAA 
               
               
                   
               
               
                 17 
                 hsa-miR- 
                 MIMAT0000770 
                 UUUGGUCCCCUUC 
                 58 
                 TAGCTGGTTGAAGGGG 
               
               
                   
                 133b 
                   
                 AACCAGCUA 
                   
                 ACCAAA 
               
               
                   
               
               
                 18 
                 hsa-miR-9- 
                 MIMAT0000441 
                 UCUUUGGUUAUC 
                 59 
                 TCATACAGCTAGATAA 
               
               
                   
                 5p 
                   
                 UAGCUGUAUGA 
                   
                 CCAAAGA 
               
               
                   
               
               
                 19 
                 hsa-miR- 
                 MIMAT0000763 
                 UCCAGCAUCAGUG 
                 60 
                 TCCAGCATCAGTGATTT 
               
               
                   
                 338-3p 
                   
                 AUUUUGUUG 
                   
                 TGTTG 
               
               
                   
               
               
                 20 
                 hsa-miR- 
                 MIMAT0000276 
                 UGAUUGUCCAAAC 
                 61 
                 TGATTGTCCAAACGCA 
               
               
                   
                 219a-5p 
                   
                 GCAAUUCU 
                   
                 ATTCT 
               
               
                   
               
               
                 21 
                 hsa-miR507 
                 MIMAT0002879 
                 UUUUGCACCUUUU 
                 62 
                 TTCACTCCAAAAGGTG 
               
               
                   
                   
                   
                 GGAGUGAA 
                   
                 CAAAA 
               
               
                   
               
               
                 22 
                 hsa-miR- 
                 MIMAT0002883 
                 AUUGACACUUCUG 
                 63 
                 ATTGACACTTCTGTGAG 
               
               
                   
                 514a-3p 
                   
                 UGAGUAGA 
                   
                 TAGA 
               
               
                   
               
               
                 23 
                 hsa-miR- 
                 MIMAT0004779 
                 UACUGCAGACAGU 
                 64 
                 TACTGCAGACAGTGGC 
               
               
                   
                 509-5p 
                   
                 GGCAAUCA 
                   
                 AATCA 
               
               
                   
               
               
                 24 
                 hsa-miR-7- 
                 MIMAT0000252 
                 UGGAAGACUAGU 
                 65 
                 AACAACAAAATCACTA 
               
               
                   
                 5p 
                   
                 GAUUUUGUUGUU 
                   
                 GTCTTCCA 
               
               
                   
               
               
                 25 
                 hsa-miR- 
                 MIMAT0000266 
                 UCCUUCAUUCCAC 
                 66 
                 CAGACTCCGGTGGAAT 
               
               
                   
                 205-5p 
                   
                 CGGAGUCUG 
                   
                 GAAGGA 
               
               
                   
               
               
                 26 
                 hsa-miR- 
                 MIMAT0000434 
                 UGUAGUGUUUCC 
                 67 
                 TCCATAAAGTAGGAAA 
               
               
                   
                 142-3p 
                   
                 UACUUUAUGGA 
                   
                 CACTACA 
               
               
                   
               
               
                 27 
                 hsa-miR- 
                 MIMAT0000232 
                 ACAGUAGUCUGCA 
                 68 
                 TAACCAATGTGCAGAC 
               
               
                   
                 199a-3p 
                   
                 CAUUGGUUA 
                   
                 TACTGT 
               
               
                   
               
               
                 28 
                 hsa-miR- 
                 MIMAT0000682 
                 UAACACUGUCUGG 
                 69 
                 ACATCGTTACCAGACA 
               
               
                   
                 200a-3p 
                   
                 UAACGAUGU 
                   
                 GTGTTA 
               
               
                   
               
               
                 29 
                 hsa-miR- 
                 MIMAT0000318 
                 UAAUACUGCCUGG 
                 70 
                 TCATCATTACCAGGCA 
               
               
                   
                 200b-3p 
                   
                 UAAUGAUGA 
                   
                 GTATTA 
               
               
                   
               
               
                 30 
                 hsa-miR- 
                 MIMAT0000222 
                 CUGACCUAUGAAU 
                 71 
                 GGCTGTCAATTCATAG 
               
               
                   
                 192-5p 
                   
                 UGACAGCC 
                   
                 GTCAG 
               
               
                   
               
               
                 31 
                 has-miR- 
                 MIMAT0000460 
                 UGUAACAGCAACU 
                 72 
                 TCCACATGGAGTTGCTG 
               
               
                   
                 194-5p 
                   
                 CCAUGUGGA 
                   
                 TTACA 
               
               
                   
               
               
                 32 
                 hsa-miR- 
                 MIMAT0001541 
                 UGGCAGUGUAUU 
                 73 
                 ACCAGCTAACAATACA 
               
               
                   
                 449a 
                   
                 GUUAGCUGGU 
                   
                 CTGCCA 
               
               
                   
               
               
                 33 
                 hsa-let-7a- 
                 MIMAT0000062 
                 UGAGGUAGUAGG 
                 74 
                 AACTATACAACCTACT 
               
               
                   
                 5p 
                   
                 UUGUAUAGUU 
                   
                 ACCTCA 
               
               
                   
               
               
                 34 
                 hsa-let-7b- 
                 MIMAT0000063 
                 UGAGGUAGUAGG 
                 75 
                 AACCACACAACCTACT 
               
               
                   
                 5p 
                   
                 UUGUGUGGUU 
                   
                 ACCTCA 
               
               
                   
               
               
                 35 
                 hsa-let-7d- 
                 MIMAT0000065 
                 AGAGGUAGUAGG 
                 76 
                 AACTATGCAACCTACT 
               
               
                   
                 5p 
                   
                 UUGCAUAGUU 
                   
                 ACCTCT 
               
               
                   
               
               
                 36 
                 hsa-let-7e- 
                 MIMAT0000066 
                 UGAGGUAGGAGG 
                 77 
                 AACTATACAACCTCCTA 
               
               
                   
                 5p 
                   
                 UUGUAUAGUU 
                   
                 CCTCA 
               
               
                   
               
               
                 37 
                 hsa-let- 
                 MIMAT0000067 
                 UGAGGUAGUAGA 
                 78 
                 AACTATACAATCTACTA 
               
               
                   
                 7f-5p 
                   
                 UUGUAUAGUU 
                   
                 CCTCA 
               
               
                   
               
               
                 38 
                 hsa-let-7g- 
                 MIMAT0000414 
                 UGAGGUAGUAGU 
                 79 
                 AACTGTACAAACTACT 
               
               
                   
                 5p 
                   
                 UUGUACAGUU 
                   
                 ACCTCA 
               
               
                   
               
               
                 39 
                 hsa-let- 
                 MIMAT0000415 
                 UGAGGUAGUAGU 
                 80 
                 AACAGCACAAACTACT 
               
               
                   
                 7i-5p 
                   
                 UUGUGCUGUU 
                   
                 ACCTCA 
               
               
                   
               
               
                 40 
                 hsa-miR- 
                 MIMAT000043 
                 UGAGAUGAAGCA 
                 81 
                 GAGCTACAGTGCTTCAT 
               
               
                   
                 143 
                 5 
                 CUGUAGCUC 
                   
                 CTCAT 
               
               
                   
               
               
                 41 
                 hsa-miR- 
                 MIMAT000024 
                 UCAGUGCACUAC 
                 82 
                 ACAAAGTTCTGTAGTG 
               
               
                   
                 148a-3p 
                 3 
                 AGAACUUUGU 
                   
                 CACTGA 
               
               
                   
               
            
           
         
       
     
     In some embodiments, a contiguous polynucleic acid described herein consists of a single cassette, wherein the single cassette encodes an RNA comprising a miRNA target site (in addition to comprising the nucleic acid sequence of the output and the nucleic acid sequence of the trans activator). 
     In other embodiments, the contiguous polynucleic acid comprises two or more cassettes, at least one of which encodes an RNA comprising a miRNA target site. 
     In some embodiments, multiple cassettes of a contiguous polynucleic acid molecule comprise at least one miRNA target site. In some embodiments, each miRNA target site of a contiguous polynucleic acid is unique (i.e., the contiguous polynucleic acid includes only one copy of the miRNA target). In some embodiments, a contiguous polynucleic acid molecule comprises at least two cassettes that each comprise at least one miRNA target site that is the same nucleic acid sequence. In some embodiments, a contiguous polynucleic acid molecule comprises at least two cassettes that each comprise at least one miRNA target site, wherein at least one miRNA target site of each cassette comprises a different nucleic acid sequence that is regulated by the same miRNA. For example, a first cassette may comprise miRNA target site X and a second cassette may comprise miRNA target site Y and miRNA Z regulates target site X and target site Y. 
     In some embodiments, a miRNA (i.e., at least one miRNA) that regulates a miRNA target site of a contiguous polynucleic acid described herein is highly expressed and/or active in at least one cell type (e.g., of a multicellular organism, such as a mammal) in which the output expression must be low. A miRNA is highly expressed and/or active, as described herein, when output expression is decreased by at least 50% relative to the level of output expression of a reference contiguous polynucleic acid (i.e., lacking the miRNA target site(s) regulated by the miRNA, but otherwise containing the identical nucleic acid sequence) in said tissue cell type. In some embodiments, output is decreased, relative to the reference contiguous polynucleic acid, by at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 99.9%. 
     In some embodiments, a miRNA (i.e., at least one miRNA) that regulates a miRNA target site of a contiguous polynucleic acid described herein is highly expressed and/or active in at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 15, at least 20, at least 25, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 500, at least 1000 cell types (e.g., of a multicellular organism, such as a mammal) in which the output expression must be low. 
     In some embodiments, a miRNA (i.e., at least one miRNA) that regulates a miRNA target of a contiguous polynucleic acid described herein has low expression and/or is inactive in at least one target cell type (e.g., of a multicellular organism, such as a mammal) in which output expression must be high. A miRNA has low expression and/or is inactive as described herein when output expression is decreased by less than 40% relative to the level of output expression of a reference contiguous polynucleic acid (i.e., lacking the miRNA target site(s) regulated by the miRNA, but otherwise containing the identical nucleic acid sequence) in said target cell type. In some embodiments, output is decreased, relative to the reference contiguous polynucleic acid, by less than 35%, less than 30%, less than 25%, less than 20%, less than 15%, less than 10%, less than 5%, less than 4%, less than 3%, less than 2%, or less than 1%. In some embodiments, there is no statistical difference between level of output expression from the contiguous polynucleic acid comprising the miRNA target and the reference continuous polynucleic acid molecule. 
     In some embodiments, a miRNA (i.e., at least one miRNA) that regulates a miRNA target site of a contiguous polynucleic acid described herein is expressed at low levels and/or inactive in at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 15, at least 20, at least 25, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 500, at least 1000 target cell types (e.g., of a multicellular organism, such as a mammal) in which the output expression must be high. 
     (ii) Exemplary Transactivators 
     Each of the contiguous polynucleic acids described herein comprises a cassette encoding an RNA (e.g., mRNA) comprising the nucleic acid sequence of a transactivator. In some embodiments, a contiguous polynucleic acid comprises the nucleic acid sequence of a single transactivator. In other embodiments, a contiguous polynucleic acid comprises the nucleic acid sequences of multiple transactivators (e.g., 2, 3, 4, 5, 6, 7, 8, 9, or 10 transactivators). 
     The terms “transactivator” or “transactivator protein,” as used herein, refer to a protein encoded on the contiguous polynucleic acid molecule that transactivates expression of an output (i.e., gene of interest) and that binds to a transactivator response element that is operably linked to the nucleic acid encoding an output (i.e., gene of interest). In some embodiments, the transactivator binds and transactivates the transactivator response element independently (i.e., in the absence of any additional factor). In other embodiments, the transactivator binds and transactivates the transactivator response element only in the presence of a transcription factor bound to the transcription factor response element. 
     In some embodiments, a transactivator protein comprises a DNA-binding domain. In some embodiments, the DNA-binding domain is engineered (i.e., not naturally-occurring) to bind a DNA sequence that is distinct from naturally-occurring sequences. Examples of DNA-binding domains are known to those having skill in the art and include, but are not limited to, DNA-binding domains derived using zinc-finger technology or TALEN technology or from mutant response regulators of two-component signaling pathways from bacteria. 
     In some embodiments, a DNA-binding domain is derived from a mammalian protein. In other embodiments a DNA binding domain is derived from a non-mammalian protein. For example, in some embodiments, a DNA-binding domain is derived from a protein originating in bacteria, yeast, or plants. In some embodiments, the DNA-binding domain requires an additional component (e.g., a protein or RNA) to target the transactivator response element. For example, in some embodiments, the DNA-binding domain is that of a CRISPR/Cas protein (e.g., Cas1, Cas2, Cas3, Cas5, Cas4, Cas6, Cas7, Cas8a, Cas8b, Cas8c, Cas9, Cas10, Cas10d, Cse1, Cse2, Csy1, Csy2, Csy3, Csm2, Cmr5, Csx10, Csx11, Csf1, Cpf1, C2c1, C2c2, C2c3) which requires the additional component of a guide RNA to target the transactivator response element. 
     In some embodiments, the transactivator protein is derived from a naturally-occurring transcription factor, wherein the DNA-binding domain of the naturally-occurring transcription factor has been mutated, resulting in an altered DNA binding specificity relative to the wild-type transcription factor. In some embodiments, the transactivator is a naturally-occurring transcription factor. 
     In some embodiments, a transactivator protein further comprises a transactivating domain (i.e., a fusion protein comprising a DNA binding domain and a transactivating domain). As used herein, the term “transactivating domain” refers to a protein domain that functions to recruit transcriptional machinery to a minimal promoter. In some embodiments, the transactivating domain does not trigger gene activation independently. In some embodiments, a transactivating domain is naturally-occurring. In other embodiments, a transactivating domain is engineered. Examples of transactivating domains are known to those having skill in the art and include, but are not limited to RelA transactivating domain, VP16, VP48, and VP64. 
     Exemplary transactivators are listed in TABLE 2. In some embodiments, the transactivator of at least one cassette is a transactivator listed in TABLE 2 or a transactivator having a least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity of its amino acid sequence with one or more transactivator listed in TABLE 2. In some embodiments, a contiguous polynucleic acid molecule described herein encodes for a combination of transactivators listed in TABLE 2 or a combination of transactivators having a least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity of its amino acid sequence with one or more transactivators listed in TABLE 2. 
     In some embodiments, the transactivator of at least one cassette is tTA, rtTA, PIT-RelA, PIT-VP16, ET-VP16, ET-RelA, NarLc-VP16, or NarLc-RelA. See e.g., Angelici B. et al., Cell Rep. 2016 Aug. 30; 16(9): 2525-2537. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Exemplary transactivators. The DNA sequences are jusl examples (hat are 
               
               
                 capable of encoding the protein sequences depicted; due to degenerate codons, very large sets 
               
               
                 of DNA sequences can encode the same protein sequence. The transactivator domains such as 
               
               
                 RclA and VP16 arc only examples of possible transactivator domains (TAD). “VP16 TAD” 
               
               
                 stands for a transactivator domain derived from a VP 16 gene of a Herpes Simplex Virus; 
               
               
                 multiple domains and their combinations and their mutants can serve as transactivalor 
               
               
                 domains when fused to DNA binding domains. The DNA binding domains (DBD) of 
               
               
                 transactivators, when derived from full-length proteins, arc merely examples of such 
               
               
                 domains; they may be further decreased or increased to include more amino acids from their 
               
               
                 full-length protein progenitor. The DBD derived from the response regulators of prokaryotic 
               
               
                 two component signaling systems arc shown based on their protein sequence in  E. coli . 
               
               
                 however, the orthologs of these genes from other prokaryotic strains and species could he 
               
               
                 used just as well. In addition. DNA binding domains of response regulators from two- 
               
               
                 component signaling pathways that do not have orthologs in  E. coli , can also be used for the 
               
               
                 same purpose. M (underlined) represents a start codon introduced in front of various DBDs to 
               
               
                 enable their translation. “::” represents a point of fusion between the DBD and TAD. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Type of 
                   
               
               
                 Seq ID 
                 Name 
                 sequence 
                 Sequence DNA/Protein 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 83 
                 RelA-TAD-1 
                 DNA 
                 ATGATGAGTTTCCCACCATGGTGTTTCCTTCTGGGCAGATCA 
               
               
                   
                   
                 sequence 
                 GCCAGGCCTCGGCCTTGGCCCCGGCCCCTCCCCAAGTCCTGC 
               
               
                   
                   
                 (example) 
                 CCCAGGCTCCAGCCCCTGCCCCTGCTCCAGCCATGGTATCAG 
               
               
                   
                   
                   
                 CTCTGGCCCAGGCCCCAGCCCCTGTCCCAGTCCTAGCCCCAG 
               
               
                   
                   
                   
                 GCCCTCCTCAGGCTGTGGCCCCACCTGCCCCCAAGCCCACCC 
               
               
                   
                   
                   
                 AGGCTGGGGAAGGAACGCTGTCAGAGGCCCTGCTGCAGCTG 
               
               
                   
                   
                   
                 CAGTTTGATGATGAAGACCTGGGGGCCTTGCTTGGCAACAG 
               
               
                   
                   
                   
                 CACAGACCCAGCTGTGTTCACAGACCTGGCATCCGTCGACA 
               
               
                   
                   
                   
                 ACTCCGAGTTTCAGCAGCTGCTGAACCAGGGCATACCTGTGG 
               
               
                   
                   
                   
                 CCCCCCACACAACTGAGCCCATGCTGATGGAGTACCCTGAG 
               
               
                   
                   
                   
                 GCTATAACTCGCCTAGTGACAGGGGCCCAGAGGCCCCCCGA 
               
               
                   
                   
                   
                 CCCAGCTCCTGCTCCACTGGGGGCCCCGGGGCTCCCCAATGG 
               
               
                   
                   
                   
                 CCTCCTTTCAGGAGATGAAGACTTCTCCTCCATTGCGGACAT 
               
               
                   
                   
                   
                 GGACTTCTCAGCCCTGCTGAGTCAGATCAGCTCCTAA 
               
               
                   
               
               
                 84 
                   
                 Protein 
                 HDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSAL 
               
               
                   
                   
                 sequence 
                 AQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFD 
               
               
                   
                   
                   
                 DEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTE 
               
               
                   
                   
                   
                 PMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDF 
               
               
                   
                   
                   
                 SSIADMDFSALLSQISS 
               
               
                   
               
               
                 85 
                 RelA-TAD-2 
                 DNA 
                 CAGGCTGGGGAAGGAACGCTGTCAGAGGCCCTGCTGCAGCT 
               
               
                   
                   
                 sequence 
                 GCAGTTTGATGATGAAGACCTGGGGGCCTTGCTTGGCAACA 
               
               
                   
                   
                 (example) 
                 GCACAGACCCAGCTGTGTTCACAGACCTGGCATCCGTCGAC 
               
               
                   
                   
                   
                 AACTCCGAGTTTCAGCAGCTGCTGAACCAGGGCATACCTGTG 
               
               
                   
                   
                   
                 GCCCCCCACACAACTGAGCCCATGCTGATGGAGTACCCTGA 
               
               
                   
                   
                   
                 GGCTATAACTCGCCTAGTGACAGGCGCACAACGCCCACCTG 
               
               
                   
                   
                   
                 ATCCGGCACCAGCACCCCTTGGAGCTCCCGGTCTCCCCAATG 
               
               
                   
                   
                   
                 GCCTCCTTTCAGGAGATGAAGACTTCTCCTCCATTGCGGACA 
               
               
                   
                   
                   
                 TGGACTTCTCAGCCCTGCTGAGTCAGATCAGCTCC 
               
               
                   
               
               
                 86 
                   
                 Protein 
                 QAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS 
               
               
                   
                   
                 sequence 
                 EFQQLINQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAP 
               
               
                   
                   
                   
                 APLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS 
               
               
                 87 
                 RelA-TAD-1 
                 DNA 
                 CCCAAGCCAGCACCCCAGCCCTATCCCTTTACGTCATCCCTG 
               
               
                   
                   
                 sequence 
                 AGCACCATCAACTATGATGAGTTTCCCACCATGGTGTTTCCT 
               
               
                   
                   
                 (example) 
                 TCTGGGCAGATCAGCCAGGCCTCGGCCTTGGCCCCGGCCCCT 
               
               
                   
                   
                   
                 CCCCAAGTCCTGCCCCAGGCTCCAGCCCCTGCCCCTGCTCCA 
               
               
                   
                   
                   
                 GCCATGGTATCAGCTCTGGCCCAGGCCCCAGCCCCTGTCCCA 
               
               
                   
                   
                   
                 GTCCTAGCCCCAGGCCCTCCTCAGGCTGTGGCCCCACCTGCC 
               
               
                   
                   
                   
                 CCCAAGCCCACCCAGGCTGGGGAAGGAACGCTGTCAGAGGC 
               
               
                   
                   
                   
                 CCTGCTGCAGCTGCAGTTTGATGATGAAGACCTGGGGGCCTT 
               
               
                   
                   
                   
                 GCTTGGCAACAGCACAGACCCAGCTGTGTTCACAGACCTGG 
               
               
                   
                   
                   
                 CATCCGTCGACAACTCCGAGTTTCAGCAGCTGCTGAACCAGG 
               
               
                   
                   
                   
                 GCATACCTGTGGCCCCCCACACAACTGAGCCCATGCTGATGG 
               
               
                   
                   
                   
                 AGTACCCTGAGGCTATAACTCGCCTAGTGACAGGGGCCCAG 
               
               
                   
                   
                   
                 AGGCCCCCCGACCCAGCTCCTGCTCCACTGGGGGCCCCGGG 
               
               
                   
                   
                   
                 GCTCCCCAATGGCCTCCTTTCAGGAGATGAAGACTTCTCCTC 
               
               
                   
                   
                   
                 CATTGCGGACATGGACTTCTCAGCCCTGCTGAGTCAGATCAG 
               
               
                   
                   
                   
                 CTCC 
               
               
                   
               
               
                 88 
                   
                 Protein 
                 PKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQ 
               
               
                   
                   
                 sequence 
                 VLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPT 
               
               
                   
                   
                   
                 QAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSE 
               
               
                   
                   
                   
                 FQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAP 
               
               
                   
                   
                   
                 LGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS 
               
               
                   
               
               
                 89 
                 VP16-TAD-1 
                 DNA 
                 GCCCCCCCGACCGATGTCAGCCTGGGGGACGAGCTCCACTT 
               
               
                   
                   
                 sequence 
                 AGACGGCGAGGACGTGGCGATGGCGCATGCCGACGCGCTAG 
               
               
                   
                   
                 (example) 
                 ACGATTTCGATCTGGACATGTTGGGGGACGGGGATTCCCCG 
               
               
                   
                   
                   
                 GGTCCGGGATTTACCCCCCACGACTCCGCCCCCTACGGCGCT 
               
               
                   
                   
                   
                 CTGGATATGGCCGACTTCGAGTTTGAGCAGATGTTTACCGAT 
               
               
                   
                   
                   
                 GCCCTTGGAATTGACGAGTACGGTGGG 
               
               
                   
               
               
                 90 
                   
                 Protein 
                 APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPG 
               
               
                   
                   
                 sequence 
                 PGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG 
               
               
                   
               
               
                 91 
                 VP16-TAD-2 
                 DNA 
                 CCGGCAGATGCCCTTGATGACTTCGATTTGGACATGCTCCCA 
               
               
                   
                   
                 sequence 
                 GCGGATGCCTTGGACGATTTTGATCTCGATATGCTTCCCGCC 
               
               
                   
                   
                 (example) 
                 GACGCACTCGATGATTTCGATCTGGATATGCTCCCGGGT 
               
               
                   
               
               
                 92 
                   
                 Protein 
                 PADALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG 
               
               
                   
                   
                 sequence 
                   
               
               
                   
               
               
                 93 
                 VP48-TAD-1 
                 DNA 
                 GGTCCGGCAGATGCCCTTGATGACTTCGATTTGGACATGCTC 
               
               
                   
                   
                 sequence 
                 CCAGCGGATGCCTTGGACGATTTTGATCTCGATATGCTTCCC 
               
               
                   
                   
                 (example) 
                 GCCGACGCACTCGATGATTTCGATCTGGATATGCTCCCGGGT 
               
               
                   
               
               
                 94 
                   
                 Protein 
                 GPADALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG 
               
               
                   
                   
                 sequence 
                   
               
               
                   
               
               
                 95 
                 PT1::RelA TAD-1 
                 DNA 
                 APGAGICGAGGAGAGGTGCGCATGGCGAAGGCAGGGCGGG 
               
               
                   
                   
                 sequence 
                 AGGGGCCGCGGGACAGCGTGTGGCTGTCGGGGGAGGGGCG 
               
               
                   
                   
                 (example) 
                 GCGCGGCGGTCGCCGTGGGGGGCAGCCGTCCGGGCTCGACC 
               
               
                   
                   
                   
                 GGGACCGGATCACCGGGGTCACCGTCCGGCTGCTGGACACG 
               
               
                   
                   
                   
                 GAGGGCCTGACGGGGTTCTCGATGCGCCGCCTGGCCGCCGA 
               
               
                   
                   
                   
                 GCTGAACGTCACCGCGATGTCCGTGTACTGGTACGTCGACAC 
               
               
                   
                   
                   
                 CAAGGACCAGTTGCTCGAGCTCGCCCTGGACGCCGTCTTCGG 
               
               
                   
                   
                   
                 CGAGCTGCGCCACCCGGACCCGGACGCCGGGCTCGACTGGC 
               
               
                   
                   
                   
                 GCGAGGAACTGCGGGCCCTGGCCCGGGAGAACCGGGCGCTG 
               
               
                   
                   
                   
                 CTGGTGCGCCACCCCTGGTCGTCCCGGCTGGTCGGCACCTAC 
               
               
                   
                   
                   
                 CTCAACATCGGCCCGCACTCGCTGGCCTTCTCCCGCGCGGTG 
               
               
                   
                   
                   
                 CAGAACGTCGTGCGCCGCAGCGGGCTGCCCGCGCACCGCCT 
               
               
                   
                   
                   
                 GACCGGCGCCATCTCGGCCGTCTTCCAGTTCGTCTACGGCTA 
               
               
                   
                   
                   
                 CGGCACCATCGAGGGCCGCTTCCTCGCCCGGGTGGCGGACA 
               
               
                   
                   
                   
                 CCGGGCTGAGTCCGGAGGAGTACTTCCAGGACTCGATGACC 
               
               
                   
                   
                   
                 GCGGTGACCGAGGTGCCGGACACCGCGGGCGTCATCGAGGA 
               
               
                   
                   
                   
                 CGCGCAGGACATCATGGCGGCCCGGGGCGGCGACACCGTGG 
               
               
                   
                   
                   
                 CGGAGATGCTGGACCGGGACTTCGAGTTCGCCCTCGACCTGC 
               
               
                   
                   
                   
                 TCGTCGCGGGCATCGACGCGATGGTCGAACAGGCCTCCGCG 
               
               
                   
                   
                   
                 TACAGCCGCGCGC::ATGATGAGTTTCCCACCATGGTGTTTCC 
               
               
                   
                   
                   
                 TTCTGGGCAGATCAGCCAGGCCTCGGCCTTGGCCCCGGCCCC 
               
               
                   
                   
                   
                 TCCCCAAGTCCTGCCCCAGGCTCCAGCCCCTGCCCCTGCTCC 
               
               
                   
                   
                   
                 AGCCATGGTATCAGCTCTGGCCCAGGCCCCAGCCCCTGTCCC 
               
               
                   
                   
                   
                 AGTCCTAGCCCCAGGCCCTCCTCAGGCTGTGGCCCCACCTGC 
               
               
                   
                   
                   
                 CCCCAAGCCCACCCAGGCTGGGGAAGGAACGCTGTCAGAGG 
               
               
                   
                   
                   
                 CCCTGCTGCAGCTGCAGTTTGATGATGAAGACCTGGGGGCCT 
               
               
                   
                   
                   
                 TGCTTGGCAACAGCACAGACCCAGCTGTGTTCACAGACCTG 
               
               
                   
                   
                   
                 GCATCCGTCGACAACTCCGAGTTTCAGCAGCTGCTGAACCAG 
               
               
                   
                   
                   
                 GGCATACCTGTGGCCCCCCACACAACTGAGCCCATGCTGATG 
               
               
                   
                   
                   
                 GAGTACCCTGAGGCTATAACTCGCCTAGTGACAGGGGCCCA 
               
               
                   
                   
                   
                 GAGGCCCCCCGACCCAGCTCCTGCTCCACTGGGGGCCCCGG 
               
               
                   
                   
                   
                 GGCTCCCCAATGGCCTCCTTTCAGGAGATGAAGACTTCTCCT 
               
               
                   
                   
                   
                 CCATTGCGGACATGGACTTCTCAGCCCTGCTGAGTCAGATCA 
               
               
                   
                   
                   
                 GCTCCTAA 
               
               
                   
               
               
                 96 
                   
                 Protein 
                 MSRGEVRMAKAGREGPRDSVWLSGEGRRGGRRGGQPSGLDR 
               
               
                   
                   
                 sequence 
                 DRITGVTVRLLDTEGLTGFSMRRLAAELNVTAMSVYWYVDTK 
               
               
                   
                   
                   
                 DQLLELALDAVFGELRHPDPDAGLDWREELRALARENRALLV 
               
               
                   
                   
                   
                 RHPWSSRLVGTYLNIGPHSLAFSRAVQNVVRRSGLPAHRLTG 
               
               
                   
                   
                   
                 AISAVFQFVYGYGTIEGRFLARVADTGLSPEEYFQDSMTAVT 
               
               
                   
                   
                   
                 EVPDTAGVIEDAQDIMAARGGDTVAEMLDRDFEFALDLLVAG 
               
               
                   
                   
                   
                 IDAMVEQASAYSRA::HDEFPTMVFPSGQISQASALAPAPPQ 
               
               
                   
                   
                   
                 VLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPK 
               
               
                   
                   
                   
                 PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASV 
               
               
                   
                   
                   
                 DNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPP 
               
               
                   
                   
                   
                 DPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS 
               
               
                   
               
               
                 97 
                 PT1::VP16 TAD-2 
                 DNA 
                 APGAGTCGAGGAGAGGTGCGCATGGCGAAGGCAGGGCGGG 
               
               
                   
                   
                 sequence 
                 AGGGGCCGCGGGACAGCGTGTGGCTGTCGGGGGAGGGGCG 
               
               
                   
                   
                 (example) 
                 GCGCGGCGGTCGCCGTGGGGGGCAGCCGTCCGGGCTCGACC 
               
               
                   
                   
                   
                 GGGACCGGATCACCGGGGTCACCGTCCGGCTGCTGGACACG 
               
               
                   
                   
                   
                 GAGGGCCfGACGGGGTTCTCGATGCGCCGCCTGGCCGCCGA 
               
               
                   
                   
                   
                 GCTGAACGTCACCGCGATGTCCGTGTACTGGTACGTCGACAC 
               
               
                   
                   
                   
                 CAAGGACCAGTTGCTCGAGCTCGCCCTGGACGCCGTCTTCGG 
               
               
                   
                   
                   
                 CGAGCTGCGCCACCCGGACCCGGACGCCGGGCTCGACTGGC 
               
               
                   
                   
                   
                 GCGAGGAACTGCGGGCCCTGGCCCGGGAGAACCGGGCGCTG 
               
               
                   
                   
                   
                 CTGGTGCGCCACCCCTGGTCGTCCCGGCTGGTCGGCACCTAC 
               
               
                   
                   
                   
                 CTCAACATCGGCCCGCACTCGCTGGCCTTCTCCCGCGCGGTG 
               
               
                   
                   
                   
                 CAGAACGTCGTGCGCCGCAGCGGGCTGCCCGCGCACCGCCT 
               
               
                   
                   
                   
                 GACCGGCGCCATCTCGGCCGTCTTCCAGTTCGTCTACGGCTA 
               
               
                   
                   
                   
                 CGGCACCATCGAGGGCCGCTTCCTCGCCCGGGTGGCGGACA 
               
               
                   
                   
                   
                 CCGGGCTGAGTCCGGAGGAGTACTTCCAGGACTCGATGACC 
               
               
                   
                   
                   
                 GCGGTGACCGAGGTGCCGGACACCGCGGGCGTCATCGAGGA 
               
               
                   
                   
                   
                 CGCGCAGGACATCATGGCGGCCCGGGGCGGCGACACCGTGG 
               
               
                   
                   
                   
                 CGGAGATGCTGGACCGGGACTTCGAGTTCGCCCTCGACCTGC 
               
               
                   
                   
                   
                 TCGTCGCGGGCATCGACGCGATGGTCGAACAGGCCTCCGCG 
               
               
                   
                   
                   
                 TACAGCCGCGCGCGTACGAAAAACAATTACGGGTCTACCAT 
               
               
                   
                   
                   
                 CGAGGGCCTGCTCGATCTCCCGGACGACGACGCCCCCGAAG 
               
               
                   
                   
                   
                 AGGCGGGGCTGGCGGCTCCGCGCCTGTCCTTTCTCCCCGCGG 
               
               
                   
                   
                   
                 GACACACGCGCAGACTGTCGACG::GCCCCCCCGACCGATGT 
               
               
                   
                   
                   
                 CAGCCTGGGGGACGAGCTCCACTTAGACGGCGAGGACGTGG 
               
               
                   
                   
                   
                 CGATGGCGCATGCCGACGCGCTAGACGATTTCGATCTGGAC 
               
               
                   
                   
                   
                 ATGTTGGGGGACGGGGATTCCCCGGGTCCGGGATTTACCCCC 
               
               
                   
                   
                   
                 CACGACTCCGCCCCCTACGGCGCTCTGGATATGGCCGACTTC 
               
               
                   
                   
                   
                 GAGTTTGAGCAGATGTTTACCGATGCCCTTGGAATTGACGAG 
               
               
                   
                   
                   
                 TACGGTGGG 
               
               
                   
               
               
                 98 
                   
                 Protein 
                 MSRGEVRMAKAGREGPRDSVWLSGEGRRGGRRGGQPSGLDR 
               
               
                   
                   
                 sequence 
                 DRITGVTVRLLDTEGLTGFSMRRLAAELNVTAMSVYWYVDTK 
               
               
                   
                   
                   
                 DQLLELALDAVFGELRHPDPDAGLDWREELRALARENRALLV 
               
               
                   
                   
                   
                 RHPWSSRLVGTYLNIGPHSLAFSRAVQNVVRRSGLPAHRITGAI 
               
               
                   
                   
                   
                 SAVFQFVYGYGTIEGRFLARVADTGLSPEEYFQDSMTAVTEVP 
               
               
                   
                   
                   
                 DTAGVIEDAQDIMAARGGDTVAEMLDRDFEFALDLLVAGIDA 
               
               
                   
                   
                   
                 MVEQASAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRL 
               
               
                   
                   
                   
                 SFLPAGHTRRLST::APPTDVSLGDELHLDGEDVAMAHADALDD 
               
               
                   
                   
                   
                 FDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDAL 
               
               
                   
                   
                   
                 GIDEYGG 
               
               
                   
               
               
                 99 
                 ET::RelA TAD-1 
                 DNA 
                 ATGCCCCGCCCCAAGCTCAAGTCCGATGACGAGGTACTCGA 
               
               
                   
                   
                 sequence 
                 GGCCGCCACCGTAGTGCTGAAGCGTTGCGGTCCCATAGAGTT 
               
               
                   
                   
                 (example) 
                 CACGCTCAGCGGAGTAGCAAAGGAGGTGGGGCTCTCCCGCG 
               
               
                   
                   
                   
                 CAGCGlTAATCCAGCGCTTCACCAACCGCGATACGCTGCTGG 
               
               
                   
                   
                   
                 TGAGGATGATGGAGCGCGGCGTCGAGCAGGTGCGGCATTAC 
               
               
                   
                   
                   
                 CTGAATGCGATACCGATAGGCGCAGGGCCGCAAGGGCTCTG 
               
               
                   
                   
                   
                 GGAATTTTTGCAGGTGCTCGTTCGGAGCATGAACACTCGCAA 
               
               
                   
                   
                   
                 CGACTTCTCGGTGAACTATCTCATCTCCTGGTACGAGCTCCA 
               
               
                   
                   
                   
                 GGTGCCGGAGCTACGCACGCTTGCGATCCAGCGGAACCGCG 
               
               
                   
                   
                   
                 CGGTGGTGGAGGGGATCCGCAAGCGACTGCCCCCAGGTGCT 
               
               
                   
                   
                   
                 CCTGCGGCAGCTGAGTTGCTCCTGCACTCGGTCATCGCTGGC 
               
               
                   
                   
                   
                 GCGACGATGCAGTGGGCCGTCGATCCGGATGGTGAGCTAGC 
               
               
                   
                   
                   
                 TGATCATGTGCTGGCTCAGATCGCTGCCATCCTGTGTTTAAT 
               
               
                   
                   
                   
                 GTTTCCCGAACACGACGATTTCCAACTCCTCCAGGCACATGC 
               
               
                   
                   
                   
                 GTCCGCGTACAGCCGCGCGC-ATGATGAGTTTCCCACCATGG 
               
               
                   
                   
                   
                 TGTTTCCTTCTGGGCAGATCAGCCAGGCCTCGGCCTTGGCCC 
               
               
                   
                   
                   
                 CGGCCCCTCCCCAAGTCCTGCCCCAGGCTCCAGCCCCTGCCC 
               
               
                   
                   
                   
                 CTGCTCCAGCCATGGTATCAGCTCTGGCCCAGGCCCCAGCCC 
               
               
                   
                   
                   
                 CTGTCCCAGTCCTAGCCCCAGGCCCTCCTCAGGCTGTGGCCC 
               
               
                   
                   
                   
                 CACCTGCCCCCAAGCCCACCCAGGCTGGGGAAGGAACGCTG 
               
               
                   
                   
                   
                 TCAGAGGCCCTGCTGCAGCTGCAGTTTGATGATGAAGACCTG 
               
               
                   
                   
                   
                 GGGGCCTTGCTTGGCAACAGCACAGACCCAGCTGTGTTCAC 
               
               
                   
                   
                   
                 AGACCTGGCATCCGTCGACAACTCCGAGTTTCAGCAGCTGCT 
               
               
                   
                   
                   
                 GAACCAGGGCATACCTGTGGCCCCCCACACAACTGAGCCCA 
               
               
                   
                   
                   
                 TGCTGATGGAGTACCCTGAGGCTATAACTCGCCTAGTGACAG 
               
               
                   
                   
                   
                 GGGCCCAGAGGCCCCCCGACCCAGCTCCTGCTCCACTGGGG 
               
               
                   
                   
                   
                 GCCCCGGGGCTCCCCAATGGCCTCCTTTCAGGAGATGAAGA 
               
               
                   
                   
                   
                 CTTCTCCTCCATTGCGGACATGGACTTCTCAGCCCTGCTGAG 
               
               
                   
                   
                   
                 TCAGATCAGCTCCTAA 
               
               
                   
               
               
                 100 
                   
                 Protein 
                 MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAA 
               
               
                   
                   
                 sequence 
                 LIQRFTNRDTILVRMMERGVEQVRHYLNAIPIGAGPQGLWEFL 
               
               
                   
                   
                   
                 QVLVRSMNTRNDFSVNYLISWYELQVPELRTLAIQRNRAVVEG 
               
               
                   
                   
                   
                 IRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDGELADHVLAQ 
               
               
                   
                   
                   
                 IAAILCLMFPEHDDFQLLQAHASAYSRA::HDEFPTMVFPSGQI 
               
               
                   
                   
                   
                 SQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPG 
               
               
                   
                   
                   
                 PPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDP 
               
               
                   
                   
                   
                 AVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLV 
               
               
                   
                   
                   
                 TGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLS 
               
               
                   
                   
                   
                 QISS 
               
               
                   
               
               
                 101 
                 ET::VP16TAD-2 
                 DNA 
                 ATGCCCCGCCCCAAGCTCAAGTCCGATGACGAGGTACTCGA 
               
               
                   
                   
                 sequence 
                 GGCCGCCACCGTAGTGCTGAAGCGTTGCGGTCCCATAGAGTT 
               
               
                   
                   
                 (example) 
                 CACGCTCAGCGGAGTAGCAAAGGAGGTGGGGCTCTCCCGCG 
               
               
                   
                   
                   
                 CAGCGlTAATCCAGCGCTTCACCAACCGCGATACGCTGCTGG 
               
               
                   
                   
                   
                 TGAGGATGATGGAGCGCGGCGTCGAGCAGGTGCGGCATTAC 
               
               
                   
                   
                   
                 CTGAATGCGATACCGATAGGCGCAGGGCCGCAAGGGCTCTG 
               
               
                   
                   
                   
                 GGAATTTTTGCAGGTGCTCGTTCGGAGCATGAACACTCGCAA 
               
               
                   
                   
                   
                 CGACTTCTCGGTGAACTATCTCATCTCCTGGTACGAGCTCCA 
               
               
                   
                   
                   
                 GGTGCCGGAGCTACGCACGCTTGCGATCCAGCGGAACCGCG 
               
               
                   
                   
                   
                 CGGTGGTGGAGGGGATCCGCAAGCGACTGCCCCCAGGTGCT 
               
               
                   
                   
                   
                 CCTGCGGCAGCTGAGTTGCTCCTGCACTCGGTCATCGCTGGC 
               
               
                   
                   
                   
                 GCGACGATGCAGTGGGCCGTCGATCCGGATGGTGAGCTAGC 
               
               
                   
                   
                   
                 TGATCATGTGCTGGCTCAGATCGCTGCCATCCTGTGTTTAAT 
               
               
                   
                   
                   
                 GTTTCCCGAACACGACGATTTCCAACTCCTCCAGGCACATGC 
               
               
                   
                   
                   
                 GTCCGCGTACAGCCGCGCGCGTACGAAAAACAATTACGGGT 
               
               
                   
                   
                   
                 CTACCATCGAGGGCCTGCTCGATCTCCCGGACGACGACGCCC 
               
               
                   
                   
                   
                 CCGAAGAGGCGGGGCTGGCGGCTCCGCGCCTGTCCTTTCTCC 
               
               
                   
                   
                   
                 CCGCGGGACACACGCGCAGACTGTCGACG::GCCCCCCCGAC 
               
               
                   
                   
                   
                 CGATGTCAGCCTGGGGGACGAGCTCCACTTAGACGGCGAGG 
               
               
                   
                   
                   
                 ACGTGGCGATGGCGCATGCCGACGCGCTAGACGATTTCGAT 
               
               
                   
                   
                   
                 CTGGACATGTTGGGGGACGGGGATTCCCCGGGTCCGGGATT 
               
               
                   
                   
                   
                 TACCCCCCACGACTCCGCCCCCTACGGCGCTCTGGATATGGC 
               
               
                   
                   
                   
                 CGACTTCGAGTTTGAGCAGATGTTTACCGATGCCCTTGGAAT 
               
               
                   
                   
                   
                 TGACGAGTACGGTGGG 
               
               
                   
               
               
                 102 
                   
                 Protein 
                 MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAA 
               
               
                   
                   
                 sequence 
                 LIQRFTNRDTILVRMMERGVEQVRHYLNAIPIGAGPQGLWEFL 
               
               
                   
                   
                   
                 QVLVRSMNTRNDFSVNYLISWYELQVPELRTLAIQRNRAVVEG 
               
               
                   
                   
                   
                 IRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDGELADHVLA 
               
               
                   
                   
                   
                 QIAAILCIMFPEHDDFQLIQAHASAYSRARTKNNYGSTIEGLLD 
               
               
                   
                   
                   
                 LPDDDAPEEAGLAAPRLSFLPAGHTRRISI::APPTDVSLGDELH 
               
               
                   
                   
                   
                 LDGEDVAMAHADALDDIDLDMLGDGDSPGPGFTPHDSAPYGA 
               
               
                   
                   
                   
                 LDMADFEFEQMFTDALGIDEYGG 
               
               
                   
               
               
                 103 
                 LEX::VP16 TAD-2 
                   
                 ATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCT 
               
               
                   
                   
                 DNA 
                 CATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGC 
               
               
                   
                   
                 sequence 
                 GTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACG 
               
               
                   
                   
                 (example) 
                 CGGNTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTT 
               
               
                   
                   
                   
                 ATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTG 
               
               
                   
                   
                   
                 CAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGC 
               
               
                   
                   
                   
                 TGCCGGTGAACCACTTCTGGCGCAACAGCATATTGAAGGTC 
               
               
                   
                   
                   
                 ATTATCAGGTCGATCCTTCCTTATTCAAGCCGAATGCTGATT 
               
               
                   
                   
                   
                 TCCTGCTGCGCGTCAGCGGGATGTCGATGAAAGATATCGGC 
               
               
                   
                   
                   
                 ATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGAT 
               
               
                   
                   
                   
                 GTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAA 
               
               
                   
                   
                   
                 GTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGA 
               
               
                   
                   
                   
                 ACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGA 
               
               
                   
                   
                   
                 CCTTCGTCAGCAGAGCTTCACCATTGAAGGTCTGGCGGTTGG 
               
               
                   
                   
                   
                 GGTTATTCGCAACGGCGACTGGCTGTCTAGCTATCCTTATGA 
               
               
                   
                   
                   
                 CGTGCCTGACTATGCCAGCCTGGGAGGATCTAGA::GCCCCCC 
               
               
                   
                   
                   
                 CGACCGATGTCAGCCTGGGGGACGAGCTCCACTTAGACGGC 
               
               
                   
                   
                   
                 GAGGACGTGGCGATGGCGCATGCCGACGCGCTAGACGATTT 
               
               
                   
                   
                   
                 CGATCTGGACATGTTGGGGGACGGGGATTCCCCGGGTCCGG 
               
               
                   
                   
                   
                 GATTTACCCCCCACGACTCCGCCCCCTACGGCGCTCTGGATA 
               
               
                   
                   
                   
                 TGGCCGACTTCGAGTTTGAGCAGATGTTTACCGATGCCCTTG 
               
               
                   
                   
                   
                 GAATTGACGAGTACGGTGGGTAGTG 
               
               
                   
               
               
                 104 
                   
                 Protein 
                 MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNA7E 
               
               
                   
                   
                 sequence 
                 EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPL 
               
               
                   
                   
                   
                 LAQQHIEGHYQVDPSIFKPNADFILRVSGMSMKDIGIMDGDLL 
               
               
                   
                   
                   
                 AVHKTQDVRNGQVVVARIDDEVTVKRIKKQGNKVELLPENSE 
               
               
                   
                   
                   
                 FKPIVVDLRQQSFTIEGIAVGVIRNGDWLSSYPYDVPDYASLGG 
               
               
                   
                   
                   
                 SR::APPTDVSIGDEIHLDGEDVAMAHADALDDFDLDMLGDGD 
               
               
                   
                   
                   
                 SPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG 
               
               
                   
               
               
                 105 
                 NarL DBD 
                 DNA 
                 ATGGCTACGACCGAGCGGGACGTAAACCAGCTTACTCCGAG 
               
               
                   
                 [NARL_ECOLI 
                 sequence 
                 AGAGAGGGACATTTTGAAGCTGATTGCGCAGGGGCTTCCCA 
               
               
                   
                 UniProtKB- 
                 (example) 
                 ATAAGATGATTGCCAGACGCCTTGATATCACGGAAAGCACT 
               
               
                   
                 P0AF28I147 
                   
                 GTGAAAGTCCACGTGAAACACATGCTCAAAAAGATGAAACT 
               
               
                   
                 -215]::VP16 
                   
                 CAAGTCCCGCGTGGAAGCTGCGGTCTGGGTACATCAGGAGC 
               
               
                   
                 TAD-2 
                   
                 GAATCTTTGGT::CCGGCAGATGCCCTTGATGACTTCGATTTG 
               
               
                   
                   
                   
                 GACATGCTCCCAGCGGATGCCTTGGACGATTTTGATCTCGAT 
               
               
                   
                   
                   
                 ATGCTTCCCGCCGACGCACTCGATGATTTCGATCTGGATATG 
               
               
                   
                   
                   
                 CTCCCGGGT 
               
               
                   
               
               
                 106 
                   
                 Protein 
                   M ATTERDVNQLTPRERDILKLIAQGLPNKMIARRLDITESTVKV 
               
               
                   
                   
                 sequence 
                 HVKHMLKKMKLKSRVEAAVWVHQERIFG::PADALDDFDLDM 
               
               
                   
                   
                   
                 LPADALDDFDLDMLPADALDDFDLDMLPG 
               
               
                   
               
               
                 107 
                 NarL DBD 
                 DNA 
                 ATGGCTACGACCGAGCGGGACGTAAACCAGCTTACTCCGAG 
               
               
                   
                 [NARL_ECOLI 
                 sequence 
                 AGAGAGGGACATTTTGAAGCTGATTGCGCAGGGGCTTCCCA 
               
               
                   
                 UniProtKB- 
                 (example) 
                 ATAAGATGATTGCCAGACGCCTTGATATCACGGAAAGCACT 
               
               
                   
                 POAF28I147 
                   
                 GTGAAAGTCCACGTGAAACACATGCTCAAAAAGATGAAACT 
               
               
                   
                 -215]::VP16 
                   
                 CAAGTCCCGCGTGGAAGCTGCGGTCTGGGTACATCAGGAGC 
               
               
                   
                 TAD-1 
                   
                 GAATCTTTGCCAGC::GCCCCCCCGACCGATGTCAGCCTGGGG 
               
               
                   
                   
                   
                 GACGAGCTCCACTTAGACGGCGAGGACGTGGCGATGGCGCA 
               
               
                   
                   
                   
                 TGCCGACGCGCTAGACGATTTCGATCTGGACATGTTGGGGG 
               
               
                   
                   
                   
                 ACGGGGATTCCCCGGGTCCGGGATTTACCCCCCACGACTCCG 
               
               
                   
                   
                   
                 CCCCCTACGGCGCTCTGGATATGGCCGACTTCGAGTTTGAGC 
               
               
                   
                   
                   
                 AGATGTTTACCGATGCCCTTGGAATTGACGAGTACGGTGGGT 
               
               
                   
                   
                   
                 GA 
               
               
                   
               
               
                 108 
                   
                 Protein 
                 MATTERDVNQLTPRERDILKLIAQGLPNKMIARRLDITESTVKV 
               
               
                   
                   
                 sequence 
                 HVKHMLKKMKLKSRVEAAVWVHQERIFAS::APPTDVSLGDEL 
               
               
                   
                   
                   
                 HLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYG 
               
               
                   
                   
                   
                 ALDMADFEFEQMFTDALGIDEYGG 
               
               
                   
               
               
                 109 
                 NarL DBD 
                 DNA 
                 ATGGCTACGACCGAGCGGGACGTAAACCAGCTTACTCCGAG 
               
               
                   
                 [NARL_ECOLI 
                 sequence 
                 AGAGAGGGACATTTTGAAGCTGATTGCGCAGGGGCTTCCCA 
               
               
                   
                 UniProtKB - 
                 (example) 
                 ATAAGATGATTGCCAGACGCCTTGATATCACGGAAAGCACT 
               
               
                   
                 POAF28I147 
                   
                 GTGAAAGTCCACGTGAAACACATGCTCAAAAAGATGAAACT 
               
               
                   
                 -215]::VP48 
                   
                 CAAGTCCCGCGTGGAAGCTGCGGTCTGGGTACATCAGGAGC 
               
               
                   
                 TAD-1 
                   
                 GAATCTTTGCCAGC::GGTCCGGCAGATGCCCTTGATGACTTC 
               
               
                   
                   
                   
                 GATTTGGACATGCTCCCAGCGGATGCCTTGGACGATTTTGAT 
               
               
                   
                   
                   
                 CTCGATATGCTTCCCGCCGACGCACTCGATGATTTCGATCTG 
               
               
                   
                   
                   
                 GATATGCTCCCGGGTTGA 
               
               
                   
               
               
                 110 
                   
                 Protein 
                 MATTERDVNQLTPRERDILKLIAQGLPNKMIARRLDITESTVKV 
               
               
                   
                   
                 sequence 
                 HVKHMLKKMKLKSRVEAAVWVHQERIFAS::GPADALDDFDL 
               
               
                   
                   
                   
                 DMLPADALDDFDLDMLPADALDDFDLDMLPG 
               
               
                   
               
               
                 111 
                 OMPR-D55E:: 
                 DNA 
                 ATGCAAGAAAACTACAAGATTCTCGTGGTGGATGATGACAT 
               
               
                   
                 VP16 TAD-2 
                 sequence 
                 GCGACTTCGCGCATTGCTCGAtAAGATATCTGACCGAGCAGG 
               
               
                   
                   
                 (example) 
                 GATTTCAAGTGCGCTCCGTGGCCAATGCCGAGCAGATGGAT 
               
               
                   
                   
                   
                 AGGCTCTTGACGAGGGAGTCGTTCCATCTGATGGTGCTGGAA 
               
               
                   
                   
                   
                 TTGATGCTTCCCGGTGAGGACGGATTGTCCATTTGCCGGAGA 
               
               
                   
                   
                   
                 CTTAGGTCGCAGTCAAACCCCATGCCGATCATCATGGTCACA 
               
               
                   
                   
                   
                 GCGAAGGGAGAGGAGGTCGATAGAATTGTAGGTCTTGAGAT 
               
               
                   
                   
                   
                 TGGGGCAGACGACTACATCCCCAAGCCGTTCAATCCCCGGG 
               
               
                   
                   
                   
                 AACTTCTTGCGCGAATCCGAGCCGTGCTCAGGCGACAGGCC 
               
               
                   
                   
                   
                 AACGAGCTGCCCGGAGCTCCATCGCAAGAGGAAGCGGTCAT 
               
               
                   
                   
                   
                 CGCGTTCGGGAAGTTCAAGTTGAACCTCGGCACGAGAGAGA 
               
               
                   
                   
                   
                 TGTTTCGGGAAGATGAACCTATGCCGCTCACATCGGGGGAG 
               
               
                   
                   
                   
                 TTTGCGGTCTTGAAAGCACTTGTCTCACACCCGAGAGAACCT 
               
               
                   
                   
                   
                 CTGTCGCGGGATAAACTCATGAATCTGGCGAGAGGCAGAGA 
               
               
                   
                   
                   
                 GTATAGCGCGATGGAAAGGTCCATCGATGTCCAGATTAGCC 
               
               
                   
                   
                   
                 GCCTCCGCCGCATGGTGGAGGAAGATCCAGCCCACCCTCGG 
               
               
                   
                   
                   
                 TACATCCAGACTGTATGGGGATTGGGGTATGTGTTCGTACCG 
               
               
                   
                   
                   
                 GATGGGTCAAAAGCAGGA::CCGGCGGACGCACTGGATGACT 
               
               
                   
                   
                   
                 TTGACTTGGATATGCTCCCAGCGGATGCGTTGGACGATTTTG 
               
               
                   
                   
                   
                 ACCTTGACATGTTGCCTGCCGACGCGCTTGACGACTTCGACT 
               
               
                   
                   
                   
                 TGGACATGCTGCCCGGT 
               
               
                   
               
               
                 112 
                   
                 Protein 
                 MQENYKILVVDDDMRLRALIERYLTIQGFQVRSVANAEQMD 
               
               
                   
                   
                 sequence 
                 RLLTRESFHLMVLELMLPGEDGLSICRRLRSQSNPMPIIMVTAK 
               
               
                   
                   
                   
                 GEEVDRIVGIEIGADDYIPKPFNPREILARIRAVLRRQANELPGA 
               
               
                   
                   
                   
                 PSQEEAVIAFGKFKLNLGTREMFREDEPMPLTSGEFAVLKALVS 
               
               
                   
                   
                   
                 HPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPA 
               
               
                   
                   
                   
                 HPRYIQTVWGLGYVFVPDGSKAG::PADALDDFDLDMLPADAL 
               
               
                   
                   
                   
                 DDFDLDMLPADALDDFDIDMLPG 
               
               
                   
               
               
                 113 
                 ArcA DBD 
                 Protein 
                 MVESYKFNGWELDINSRSLIGPDGEQYKLPRSEFRAMLHFCENP 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 GKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDTPEI 
               
               
                   
                 P0A9QI1134 
                   
                 IATiHGEGYRFCG::PADALDDFDLDMLPADALDDFDLDMLPAD 
               
               
                   
                 -234]::VP16 
                   
                 ALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 114 
                 AtoC DBD 
                 Protein 
                 MQLQSMKKEIRHLHQAISTSWQWGHIITNSPAMMDICKDTAK 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 IALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALP 
               
               
                   
                 Q060651121- 
                   
                 ESLLESELFGHEKGAFTGAQTLRQGLFERANEGTLLLDEIGEMP 
               
               
                   
                 461]::VP16 
                   
                 LVLQAKLLRILQEREFERIGGHQTIKVDIRIIAATNRDLQAMVKE 
               
               
                   
                 TAD-2 
                   
                 GTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKFSSENQR 
               
               
                   
                   
                   
                 DIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSED 
               
               
                   
                   
                   
                 LPPQIRQPVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQ 
               
               
                   
                   
                   
                 EGNRTRTALMLGISRRALMYKLQEYGIDPADV::PADALDDFDL 
               
               
                   
                   
                   
                 DMLPADALDDFDLDMLPADALDDFDLDML 
               
               
                   
               
               
                 115 
                 BaeR DBD 
                 Protein 
                 MQRELQQQDAESPLiIDEGRFQASWRGKMLDLTPAEFRLLKTL 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 SHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLD 
               
               
                   
                 P69228I131- 
                   
                 AEQSFIRAVYGVGYRWEA::PADALDDFDLDMLPADALDDFDL 
               
               
                   
                 234]::VP16 
                   
                 DMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 116 
                 PhoB DBD 
                 Protein 
                 MEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEFKLLHFFMTHP 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 ERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHD 
               
               
                   
                 POAFJ5I129- 
                   
                 RMVQTVRGTGYRFST::PADALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 227]:: VP16 
                   
                 PADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 117 
                 EvgA DBD 
                 Protein 
                 MNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGK 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 DNNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNK 
               
               
                   
                 P0ACZ4I118 
                   
                 IG::PADALIJDIDLDMLPADALDDFDLIMLPADALDDFDLDML 
               
               
                   
                 -204]::VP16 
                   
                   
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 118 
                 NtrC DBD 
                 Protein 
                 MSHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSIS 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 VLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIES 
               
               
                   
                 P0AFB81120 
                   
                 ELFGHEKGAFTGANTIRQGRFEQADGGTLFLDEIGDMPLDVQT 
               
               
                   
                 -469]::VP16 
                   
                 RLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGK 
               
               
                   
                 TAD-2 
                   
                 FREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELGVE 
               
               
                   
                   
                   
                 AKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLI 
               
               
                   
                   
                   
                 QDLPGELFESTVAESTSQMQPDSWATLLAQWADRALRSGHQN 
               
               
                   
                   
                   
                 LLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKL 
               
               
                   
                   
                   
                 KELGME::PADALDDFDLDMLPADALDDFDLDMLPADALDDFD 
               
               
                   
                   
                   
                 LDML 
               
               
                   
               
               
                 119 
                 NarP DBD 
                 Protein 
                 MGSKVFSERVNQYLREREMFGAEEDPFSVLTERELDVLHELAQ 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 GLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQ 
               
               
                   
                 P31802I125- 
                   
                 RGAQ::PADALDDFDLDMLPADALDDFDLDMLPADALDDFDLD 
               
               
                   
                 2i5]::VP16 
                   
                 ML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 120 
                 BasR DBD 
                 Protein 
                 MRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYAL 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 LSRLMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDK 
               
               
                   
                 P30843I117- 
                   
                 VGKARIRTVRGFGYMLVANEEN::PADALDDFDLDMLPADALD 
               
               
                   
                 222]::VP16 
                   
                 DIDLDMLPADALDDFLLIML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 121 
                 BtsR DBD 
                 Protein 
                 MQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSS 
               
               
                   
                 [UniProtKB 
                 sequence 
                 RMSGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAH 
               
               
                   
                 POAFT5I117 
                   
                 LQEIRLEDNGQAELILRNGLTVPVSRRYLKSLKEAIGL::PADAL 
               
               
                   
                 -239]::VP16 
                   
                 DDFDLDMLPADALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 122 
                 CpxR DBD 
                 Protein 
                 MRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLE 
               
               
                   
                 [UniProtKB 
                 sequence 
                 LTGTEFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDM 
               
               
                   
                 POAE88I116 
                   
                 HISNLRRKLPDRKDGHPWIKTLRGRGYIMVSAS::PADALDDFD 
               
               
                   
                 -232]::VP16 
                   
                 LDMLPADALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 123 
                 CreB DBD 
                 Protein 
                 MRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLALTRYEFLLL 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 KTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAK 
               
               
                   
                 P08368I116- 
                   
                 LRAINPDLSPINTHRGMGYSLRGL:PADALDDFDLDMLPADAL 
               
               
                   
                 232]::VP16 
                   
                 DDFDLDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 124 
                 CusR DBD 
                 Protein 
                 MRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLL 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 EFFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKI 
               
               
                   
                 POACZ8I117 
                   
                 DNDFEPKLTQTVRGVGYMLEVPDGQ::PADALDDFDLDMLPAD 
               
               
                   
                 -227]::VP16 
                   
                 ALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 125 
                 DcuR DBD 
                 Protein 
                 MQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLTPQ 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 TLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYIIWIVNC 
               
               
                   
                 POAD01I122 
                   
                 HILFTSIHYGVTGRPVYRYRIQMHYSILKQYCQ::PADALDDFD 
               
               
                   
                 -239]::VP16 
                   
                 LDMLPADALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 126 
                 DpiA DBD 
                 Protein 
                 MQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGIDPLTL 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 NAVRKLFKEPGVQHTA 
               
               
                   
                 POAEF4I123 
                   
                 ETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGRPQR1YHS 
               
               
                   
                 -226]::VP16 
                   
                 G::PADALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 127 
                 GlrR DBD 
                 Protein 
                 QQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVLING 
               
               
                   
                 [UniProtKB 
                 sequence 
                 QSGTGKEIFAQAIHNA 
               
               
                   
                 POAFU4I122 
                   
                 SPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQ 
               
               
                   
                 -444]::VP16 
                   
                 AAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIN 
               
               
                   
                 TAD-2 
                   
                 VRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTED 
               
               
                   
                   
                   
                 IPLLANHLLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQ 
               
               
                   
                   
                   
                 LVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFE 
               
               
                   
                   
                   
                 LNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDAND 
               
               
                   
                   
                   
                 FKE::PADALDDFDLDMLPADALDDFDLDMLPADALDDFDLDM 
               
               
                   
                   
                   
                 L 
               
               
                   
               
               
                 128 
                 HprR DBD 
                 Protein 
                 MQHHALNSTLEISGLRMDSVSHSVSRDNISITLTRKEFQLLWLL 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 ASRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFP 
               
               
                   
                 P76340I116- 
                   
                 EKLIATIRGMGSIVAVKK::PADALDDFDLDMLPADALDDFDL 
               
               
                   
                 223]::VP16 
                   
                 DMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 129 
                 PhoP DBD 
                 Protein 
                 MRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 LIRNNGKVVSKDSLMLQLYPDAELRESHT1DVLMGRLRKKIQA 
               
               
                   
                 P23836I117- 
                   
                 QYPQEVITTVRGQGYLFELR::PADALDDFDLDMLPADALDDFD 
               
               
                   
                 223]::VP16 
                   
                 LDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 130 
                 QseB DBD 
                 Protein 
                 MRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLE 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 LLMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKL 
               
               
                   
                 P52076I117- 
                   
                 GSDFIRTVHGIGYTLGEK::PADALDDFDLDMLPADALDDFDLD 
               
               
                   
                 219]::VP16 
                   
                 MLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 131 
                 RcsB 
                 Protein 
                 MGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLRLFAEGFLV 
               
               
                   
                 UniProtKB- 
                 sequence 
                 TEIAKKLNRSIK 
               
               
                   
                 PODMC7 
                   
                 TISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD::PADALD 
               
               
                   
                 (RCSB_EC 
                   
                 DFDLDMLPADALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 OLI) 
                   
                   
               
               
                   
                 DBD::VP16 
                   
                   
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 132 
                 RstA DBD 
                 Protein 
                 MRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTE 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 ISLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSV 
               
               
                   
                 P52108I125- 
                   
                 DVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE::PADAL 
               
               
                   
                 216]::VP16 
                   
                 DDFDLDMLPADALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 133 
                 UhpA DBD 
                 Protein 
                 MTGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEI 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 AAELGLSPKTVHVHRANLMEKIGVSNDVEIARRMFDGW::PA 
               
               
                   
                 POAGA6I11 
                   
                 DALDDFDLDMIPADALDDFDLDMLPADALDDFDIDML 
               
               
                   
                 7- 
                   
                   
               
               
                   
                 196]::VP16 
                   
                   
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 134 
                 YpdB DBD 
                 Protein 
                 MAAWQQQQTSSTPAATVTRENDTLNLVKDERIIVTPINDIYYAE 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 AHEKMTFVYTRRESYVMPMNTTEFCSKLPPSHFFRCHRSFCVNL 
               
               
                   
                 POAE39I117 
                   
                 NK1REIEPWFNNTYILRLKDLDFEVPVSRSKVKEFRQLMHL::PA 
               
               
                   
                 -244]::VP16 
                   
                 DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML 
               
               
                   
                 TAD-2 
                   
                   
               
               
                   
               
               
                 135 
                 ZraR DBD 
                 Protein 
                 MHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPSEAT 
               
               
                   
                 [UniProtKB- 
                 sequence 
                 VLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLES 
               
               
                   
                 P14375I122- 
                   
                 ELFGHEKGAFTGADKRREGRFVEADGGTLFLDEIGDISPMMQV 
               
               
                   
                 441]::VP16 
                   
                 RLLRAIQEREVQRVGSNQIISVDVRLIAATHRDLAAEVNAGRFR 
               
               
                   
                 TAD-2 
                   
                 QDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAV 
               
               
                   
                   
                   
                 KGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISEREL 
               
               
                   
                   
                   
                 PLAIASTPIPLGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQ 
               
               
                   
                   
                   
                 LGITRKTLLAKLSR::PADALDDFDLDMLPADALDDFDLDMLPA 
               
               
                   
                   
                   
                 DALDDFDLDML 
               
               
                   
               
               
                 136 
                 HSFY1 
                 Protein 
                 MAHVSSETQDVSPKDELTASEASTRSPLCEHTFPGDSDLRSMIE 
               
               
                   
                 UniProtKB- 
                 sequence 
                 EHAFQVLSQGSLLESPSYTVCVSEPDKDDDFLSLNFPRKLWKIV 
               
               
                   
                 Q96LI6(HS 
                   
                 ESDQFKSISWDENGTCIVINEELFKKEILETKAPYRIFQTDAIKSF 
               
               
                   
                 FY1_HUMAN) 
                   
                 VRQLNLYGFSKIQQNFQRSAFLATFLSEEKESSVLSKLKFYYNP 
               
               
                   
                   
                   
                 NFKRGYPQLLVRVKRRIGVKNASPISTLFNEDFNKKHFRAGAN 
               
               
                   
                   
                   
                 MENHNSAlAAEASEESLFSASKNINMPLTRESSVRQIIANSSVPI 
               
               
                   
                   
                   
                 RSGFPPPSPSTSVGPSEQIATDQHAILNQLTTIHMHSHSTYMQAR 
               
               
                   
                   
                   
                 GHIVNFITTTTSQYHIISPLQNGYFGLTVEPSAVPTRYPLVSVNE 
               
               
                   
                   
                   
                 APYRNMLPAGNPWLQMPIIADRSAAPHSRLALQPSPLDKYHPN 
               
               
                   
                   
                   
                 YN 
               
               
                   
               
               
                 137 
                 OLIG3 
                 Protein 
                 MNSDSSSVSSRASSPDMDEMYLRDHHHRHHHHQESRLNSVSST 
               
               
                   
                 UniProtKB- 
                 sequence 
                 QGDMMQKMPGESLSRAGAKAAGESSKYKIKKQLSEQDLQQLR 
               
               
                   
                 Q7RTU3 
                   
                 LKINGRERKRMHDLNLAMDGLREVMPYAHGPSVRKLSKIATL 
               
               
                   
                 (OLIG3_ 
                   
                 LLARNYILMLTSSLEEMKRLVGEIYGGHHSAFHCGTVGHSAGH 
               
               
                   
                 HUMAN) 
                   
                 PAHAANSVHPVHPILGGALSSGNASSPLSAASLPAIGTIRPPHSL 
               
               
                   
                   
                   
                 LKAPSTPPALQLGSGFQHWAGLPCPCTICQMPPPPHLSALSTAN 
               
               
                   
                   
                   
                 MARLSAESKDLLK 
               
               
                   
               
               
                 138 
                 MSGN1 
                   
                 MDNLRETFLSLEDGLGSSDSPGLLSSWDWKDRAGPFELNQASP 
               
               
                   
                 UniProtKB- 
                   
                 SQSLSPAPSLESYSSSPCPAVAGLPCEHGGASSGGSEGCSVGGAS 
               
               
                   
                 A6NI15 
                   
                 GLVEVDYNMLAFQPTHLQGGGGPKAQKGTKVRMSVQRRRKA 
               
               
                   
                 (MSGN1_ 
                   
                 SEREKLRMRTLADALHTLRNYLPPVYSQRGQPLTKIQTLKYTIK 
               
               
                   
                 HUMAN) 
                   
                 YIGELTDLLNRGREPRAQSA 
               
               
                   
               
            
           
         
       
     
     (iii) Exemplary Output Molecules 
     Each of the contiguous polynucleic acids described herein comprises a cassette encoding an RNA (e.g., mRNA) comprising the nucleic acid sequence of an output (i.e., a gene of interest). In some embodiments, a contiguous polynucleic acid comprises the nucleic acid sequence of a single output. In other embodiments, a contiguous polynucleic acid comprises the nucleic acid sequences of multiple outputs (e.g., 2, 3, 4, 5, 6, 7, 8, 9, or 10 outputs). 
     In some embodiments, the output is an RNA molecule. In some embodiments, the RNA molecule is an mRNA encoding for a protein. In some embodiments, the output is a non-coding RNA molecule. Examples of non-coding RNA molecules are known to those having skill in the art and include, but are not limited to, include transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), miRNAs, siRNAs, piRNAs, snoRNAs, snRNAs, exRNAs, scaRNAs, and long ncRNAs. 
     In some embodiments, the output is a therapeutic molecule (i.e., related to the treatment of disease), such as a therapeutic protein or RNA molecule. Examples of therapeutic molecules include, but are not limited to, antibodies (e.g., monoclonal or polyclonal; chimeric; humanized; including antibody fragments and antibody derivatives (bispecific, trispecific, scFv, and Fab)), enzymes, hormones, inflammatory molecules, anti-inflammatory molecules, immunomodulatory molecules, anti-cancer molecules, short-hairpin RNAs, short interfering RNAs and miRNAs. Specific examples of the foregoing classes of therapeutic molecules are known in the art, any of which may be used in accordance with the present disclosure. 
     In some embodiments, the output encodes for an antigen protein, protein domain, or peptide derived from a pathogen and known to elicit an immune response when produced in the body. 
     In some embodiments, the output is a detectable protein, such as a fluorescent protein. 
     In some embodiments, the output is a cytotoxin. As used herein, the term “cytotoxin” refers to a substance that is toxic to a cell. For example, in some embodiments, the output is a cytoxic protein. Examples of cytotoxic proteins are known to those having skill in the art and include, but are not limited to, granulysin, perforin/granzyme B, and the Fas/Fas ligand. 
     In some embodiments, the output is an enzyme that catalyzes activation of a prodrug. Examples of enzymes that catalyze prodrug activation are known to those having skill in the art, and include, but are not limited to carboxylesterases, acetylcholinesterases, butyrlylcholinesterases, paraxonases, matrix metalloproteinases, alkaline phosphatases, β-glucuronidases, valacyclovirases, prostate-specific antigens, purine-nucleoside phosphorylases, carboxypeptidases, amidases, β-lactamases, β-galactosidases, and cytosine deaminases. See e.g., Yang Y. et al., Enzyme-mediated hydrolytic activation of prodrugs. Acta. Pharmaceutica. Sinica B. 2011 October; 1(3): 143-159. Likewise, various prodrugs are known to those having skill in the art and include, but are not limited to, acyclovir, allopurinol, azidothymidine, bambuterol, bacampicillin, capecitabine, captopril, carbamazepine, carisoprodol, cyclophosphamide, diethylstilbestrol diphosphate, dipivefrin, enalapril, famciclovir, fludarabine triphosphate, fluorouracil, fosamprenavir, fosphentoin, fursultiamine, gabapentin encarbil, ganciclovir, gemcitabine, hydrazide MAO inhibitors, leflunomide, levodopa, methanamine, mercaptopurine, mitomycin, molsidomine, nabumetone, olsalazine, omeprazole, paliperidone, phenacetin, pivampicillin, primidone, proguanil, psilocybin, ramipril, S-methyldopa, simvastatin, sulfasalazine, sulindac, tegafur, terfenadine, valacyclovir, valganciclovir, and zidovudine. 
     In some embodiments, the output is HSV-TK, a thymidine kinase from Human alphaherpesvirus 1 (HHV-1), UniProtKB—Q9QNF7 (KITH_HHV1). 
     In some embodiments, the output is an immunomodulatory protein and/or RNA. As used herein, the term “immunomodulatory protein” (or immunomodulatory RNA) refers to a protein (or RNA) that modulates (stimulates (i.e., an immunostimulatory protein or RNA) or inhibits, (i.e., an immunoinhibitory protein or RNA)) the immune system by inducing activation and/or increasing activity of immune system components. Various immunomodulatory proteins are known to those having skill in the art. See e.g., Shahbazi S. and Bolhassani A. Immunostimulants: Types and Funtions. J. Med. Microbiol. Infec. Dis. 2016; 4(3-4): 45-51. In some embodiments, the immunomodulatory protein is a cytokine, chemokine (e.g., IL-2, IL-5, IL-6, IL-10, IL-12, IL-13, IL-15, IL-18, CCR3, CXCR3, CXCR4, and CCR10) or a colony stimulating factor. 
     In some embodiments, the output is a DNA-modifying factor. As used herein the term “DNA-modifying factor” refers to a factor that alters the structure of DNA and/or alters the sequence of DNA (e.g., by inducing recombination or introduction of mutations). In some embodiments, the DNA-modifying factor is a gene encoding a protein intended to correct a genetic defect, a DNA-modifying enzyme, and/or a component of a DNA-modifying system. In some embodiments, the DNA-modifying enzyme is a site-specific recombinase, homing endonuclease, or a protein component of a CRISPR/Cas DNA modification system. 
     In some embodiments, the output is a cell-surface receptor. In some embodiments, the output is a kinase. 
     In some embodiments, the output is a gene expression-regulating factor. The term “gene expression-regulating factor,” as used herein, refers to any factor that, when present, increases or decreases transcription of at least one gene. In some embodiments, the gene expression-regulating factor is a protein. In some embodiments, the gene expression-regulating factor is an RNA. In some embodiments, the gene expression-regulating factor is a component of a multi-component system capable of regulating gene expression. 
     In some embodiments, the output is an epigenetic modifier. The term “epigenetic modifier,” as used herein, refers to a factor (e.g., protein or RNA) that increases, decreases, or alters an epigenetic modification. Examples of epigenetic modifications are known to those of skill in the art and include, but are not limited to, DNA methylation and histone modifications. 
     In some embodiments, the output is a factor necessary for vector replication. Examples of factors necessary for vector replication are known to those having skill in the art. 
     (iv) Regulatory Component 
     A cassette encoding an RNA (e.g., comprising the nucleic acid sequence of an output and/or a transactivator) may further comprise a regulatory component. As described herein, a regulatory component is a nucleic acid sequence that controls expression of (i.e., stimulates increased or decreased expression of) the RNA. For example, in some embodiments, a cassette described herein may encode an RNA that is operably linked to a transactivator response element, a transcription factor response element, a minimal promoter, and/or a promoter element. A regulatory component is “operably linked” to a nucleic acid encoding an RNA when it is in a correct functional location and orientation in relation to the nucleic acid sequence such that it regulates (or drives) transcriptional initiation and/or expression of that sequence. 
     In some embodiments, the regulatory component comprises a transactivator response element. The “transactivator response element” can comprise a minimal DNA sequence that is bound and recognized by a transactivator protein. In some embodiments the transactivator response elements comprises more than one copy (i.e., repeats) of a minimal DNA sequence that is bound and recognized by a transactivator protein. In some embodiments, a transactivator response element comprises at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 repeats of a minimal DNA sequence that is bound and recognized by a transactivator protein. In some embodiments the repeats are tandem repeats. In some embodiments, the transactivator response element comprises a combination of minimal DNA sequences. In some embodiments, minimal DNA sequences are interspersed with spacer sequences. In some embodiments, a spacer sequence is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or greater than 20 nucleotides in length. 
     In some embodiments, the transactivator response element comprises deviations from the minimal DNA sequence, or is flanked by additional DNA sequence, while still being able to bind a transactivator protein. In some embodiments, different transactivator response elements can be placed next to each other, while all being able to bind to the same transactivator protein. 
     Exemplary transactivator response elements are listed in TABLE 3. In some embodiments, a transactivator response element consists of a nucleic acid sequence listed in TABLE 3 or a nucleic acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identity to a nucleic acid sequence listed in TABLE 3. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Exemplary transactivator response elements. ″::″ represents fusion point 
               
               
                 between the transactivator domain (TAD) and the DNA binding domain (DBD). Shorthand 
               
               
                 notation of sequences of TADs and DBDs correspond to TABLE 2. DNA sequences use the 
               
               
                 following nomenclature: W = A or T; S = C or G; K = A or C; M = G or T; Y = A or G; R = C 
               
               
                 or T; V = C,G, or T; H = A, G or T; D = A, C or T; B = A, C, or G; N = A,C,G, or T. Capital 
               
               
                 letter represent strong conservation; low-case symbol represents weaker conservation. 
               
            
           
           
               
               
               
            
               
                   
                 Examples 
                 Examples of transactivators 
               
               
                 SeqID 
                 of Transactivator response element 
                 capable of binding the sequence 
               
               
                   
               
               
                 139 
                 GAAATAGCGCTGTACAGCGTATGGGAATCTCT 
                 PIT::RELA TAD-1, PIT::RELA 
               
               
                   
                 TGTACGGTGTACGAGTATCTTCCCGTACACCGT 
                 TAD-2, PIT::RELA TAD-2, 
               
               
                   
                 AC 
                 PIT::RELA TAD-3, PIT::VP16 
               
               
                   
                   
                 TAD-1, PIT::VP16 TAD-2 
               
               
                   
               
               
                 140 
                 CATGTGATTGAATATAACCGACGTGACTGTTA 
                 ET::RELA TAD-1, ET::RELA 
               
               
                   
                 CATTTAGOGG 
                 TAD-2, ET::RELA TAD-3, 
               
               
                   
                   
                 ET::VP16 TAD-1, ET::VP16 TAD- 
               
               
                   
                   
                 2 
               
               
                   
               
               
                 141 
                 TACTGTATATATATACAGTATACTGTATATATA 
                 Lex::RELA TAD-1, Lex::RELA 
               
               
                   
                 TACAGTA 
                 TAD-2, Lex::RELA TAD-3, 
               
               
                   
                   
                 Lex::VP16 TAD-1, Lex::VP16 
               
               
                   
                   
                 TAD-2 
               
               
                   
               
               
                 142 
                 TACCCCTATAGGGGTATAGCGCCGGCTACCCC 
                 NarL DBD::RELA TAD-1, NarL 
               
               
                   
                 TATAGGGGTAT 
                 DBD::RELA TAD-2, NarL 
               
               
                 143 
                 TACCCCTATAGGGGTATAGCGCCGGCTACCCC 
                 DBD::RELA TAD-3, NarL 
               
               
                   
                 TATAGGGGTATTACCCCTATAOGGGTATAGCG 
                 DBD::VP16 TAD-1, NarL 
               
               
                   
                 CCGGCTACCCCTATAGGGGTA 
                 DBD::VP16 TAD-2 
               
               
                 144 
                 wakrrkTA 
                   
               
               
                   
               
               
                 145 
                 ATTTACATTTTGAAACATCTA 
                 OMPR-D55E::RELA TAD-1, 
               
               
                 146 
                 wAhaTGwWACmAArwdTww 
                 OMPR-D55E::RELA TAD-2, 
               
               
                   
                   
                 OMPR-D55E::RELA TAD-3, 
               
               
                   
                   
                 OMPR-D55E::VP16 TAD-1, 
               
               
                   
                   
                 OMPR-D55E::VP16 TAD-2 
               
               
                   
               
               
                 147 
                 ATGTTAATAA 
                 ArcA DBD::RELA TAD-1, ArcA 
               
               
                 148 
                 ATGTTAATAATATGTGGCATAAGCGTTAAATG 
                 DBD::RELA TAD-2, ArcA 
               
               
                 149 
                 wamawwTwrTTAAma 
                 DBD::RELA TAD-3, ArcA 
               
               
                   
                   
                 DBD::VP16 TAD-1, ArcA 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 150 
                 GCTATGCAGAAATTTGCACA 
                 AtoC DBD::RELA TAD-1, AtoC 
               
               
                   
                   
                 DBD::RELA TAD-2, AtoC 
               
               
                   
                   
                 DBD::RELA TAD-3, AtoC 
               
               
                   
                   
                 DBD::VP16 TAD-1, AtoC 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 151 
                 TTCTYCMYdATYKSYkS 
                 BaeR DBD::RELA TAD-1, BaeR 
               
               
                   
                   
                 DBD::RELA TAD-2, BaeR 
               
               
                   
                   
                 DBD::RELA TAD-3, BaeR 
               
               
                   
                   
                 DBD::VP16 TAD-1, BaeR 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 152 
                 TGTCATAAAACTGTCATATTCCTTACATATAAC 
                 PhoB DBD::RELA TAD-1, PhoB 
               
               
                   
                 TGTCA 
                 DBD::RELA TAD-2, PhoB 
               
               
                 153 
                 cTgwcAyAAAwcTgwm 
                 DBD::RELA TAD-3, PhoB 
               
               
                   
                   
                 DBD::VP16 TAD-1, PhoB 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 154 
                 TTCTTACGCCTGTAGGATTAGTAAGAA 
                 EvgA DBD::RELA TAD-1, EvgA 
               
               
                 155 
                 TkCYTACAmCTGTARGA 
                 DBD::RELA TAD-2, EvgA 
               
               
                   
                   
                 DBD::RELA TAD-3, EvgA 
               
               
                   
                   
                 DBD::VP16 TAD-1, EvgA 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 156 
                 TGCACCAWWWTGGTGCA 
                 NtrC DBD::RELA TAD-1, NtrC 
               
               
                 157 
                 tGCmCyAaaATsGtGCA 
                 DBD::RELA TAD-2, NtrC 
               
               
                   
                   
                 DBD::RELA TAD-3, NtrC 
               
               
                   
                   
                 DBD::VP16 TAD-1, NtrC 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 158 
                 NTACCCCTA 
                 1. NarPDBD::RELA TAD- 
               
               
                 159 
                 mTACyycT 
                 1, NarP DBD::RELA TAD-2, NarP 
               
               
                   
                   
                 DBD::RELA TAD-3, NarP 
               
               
                   
                   
                 DBD::VP16 TAD-1, NarP 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 160 
                 CTTAAGGTTNNCTTAAGGTT 
                 2. BasRDBD::RELA TAD- 
               
               
                   
                   
                 1, BasR DBD::RELA TAD-2, BasR 
               
               
                   
                   
                 DBD::RELA TAD-3, BasR 
               
               
                   
                   
                 DBD::VP16 TAD-1, BasR 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 161 
                 ANCNCTAAANT 
                 BtsR DBD::RELA TAD-1, BtsR 
               
               
                   
                   
                 DBD::RELA TAD-2, BtsR 
               
               
                   
                   
                 DBD::RELA TAD-3, BtsR 
               
               
                   
                   
                 DBD::VP16 TAD-1, BtsR 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 162 
                 GTAAANNNNNGTAAA 
                 CpxR DBD::RELA TAD-1, CpxR 
               
               
                 163 
                 GTAAArmwrygwaAr 
                 DBD::RELA TAD-2, CpxR 
               
               
                   
                   
                 DBD::RELA TAD-3, CpxR 
               
               
                   
                   
                 DBD::VP16 TAD-1, CpxR 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 164 
                 TTCACNNNNNNTTCAC 
                 CreB DBD::RELA TAD-1, CreB 
               
               
                   
                   
                 DBD::RELA TAD-2, CreB 
               
               
                   
                   
                 DBD::RELA TAD-3, CreB 
               
               
                   
                   
                 DBD::VP16 TAD-1, CreB 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 165 
                 AAAATGACAANNTTGTCATTTTT 
                 CusR DBD::RELA TAD-1, CusR 
               
               
                   
                   
                 DBD::RELA TAD-2, CusR 
               
               
                   
                   
                 DBD::RELA TAD-3, CusR 
               
               
                   
                   
                 DBD::VP16 TAD-1, CusR 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 166 
                 TGATTACAAAACTTTAAAAAGTGCTG 
                 DcuR DBD::RELA TAD-1, DcuR 
               
               
                 167 
                 TGATTACAAAACTTTAAAAAGTGCTGCATAGC 
                 DBD::RELA TAD-2, DcuR 
               
               
                   
                 GCCGGCCGCGCCTGATTACAAAACTTTAAAAA 
                 DBD::RELA TAD-3, DcuR 
               
               
                   
                 GTGCTG 
                 DBD::VP16 TAD-1, DcuR 
               
               
                 168 
                 TGATTACAAAACTTTAAAAAGTGCTGTAGCGC 
                 DBD::VP16 TAD-2 
               
               
                   
                 CGGCTGATTACAAAACTTTAAAAAGTGCTG 
                   
               
               
                 169 
                 TkwwTTwAaTTwykwwA 
                   
               
               
                   
               
               
                 170 
                 GATCTATTCTTTT 
                 DpiA DBD::RELA TAD-1, DpiA 
               
               
                 171 
                 TATCTTTTTTTAT 
                 DBD::RELA TAD-2, DpiA 
               
               
                   
                   
                 DBD::RELA TAD-3, DpiA 
               
               
                   
                   
                 DBD::VP16 TAD-1, DpiA 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 172 
                 TGTCN 1-10 GACA 
                 GlrR DBD::RELA TAD-1, GlrR 
               
               
                   
                   
                 DBD::RELA TAD-2, GlrR 
               
               
                   
                   
                 DBD::RELA TAD-3, GlrR 
               
               
                   
                   
                 DBD::VP16 TAD-1, GlrR 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 173 
                 CATTACAANTTGTAATG 
                 HprR DBD::RELA TAD-1, HprR 
               
               
                   
                   
                 DBD::RELA TAD-2, HprR 
               
               
                   
                   
                 DBD::RELA TAD-3, HprR 
               
               
                   
                   
                 DBD::VP16 TAD-1, HprR 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 174 
                 CATGAANNNNNTGTTTA 
                 PhoP DBD::RELA TAD-1, PhoP 
               
               
                 175 
                 WrTTTAkswwyyGTTtA 
                 DBD::RELA TAD-2, PhoP 
               
               
                   
                   
                 DBD::RELA TAD-3, PhoP 
               
               
                   
                   
                 DBD::VP16 TAD-1, PhoP 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 176 
                 rTTAAmNNNNNrTTAAm 
                 QseB DBD::RELA TAD-1, QseB 
               
               
                   
                   
                 DBD::RELA TAD-2, QseB 
               
               
                   
                   
                 DBD::RELA TAD-3, QseB 
               
               
                   
                   
                 DBD::VP16 TAD-1, QseB 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 177 
                 TAAGAATATTCCTA 
                 RcsB DBD::RELA TAD-1, RcsB 
               
               
                 178 
                 AwYmGAyKWwTYT 
                 DBD::RELA TAD-2, RcsB 
               
               
                   
                   
                 DBD::RELA TAD-3, RcsB 
               
               
                   
                   
                 DBD::VP16 TAD-1, RcsB 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 179 
                 NNTACANNNNNNTACTNN 
                 RstA DBD::RELA TAD-1, RstA 
               
               
                 180 
                 KWCWTWTvGTTACA 
                 DBD::RELA TAD-2, RstA 
               
               
                   
                   
                 DBD::RELA TAD-3, RstA 
               
               
                   
                   
                 DBD::VP16 TAD-1, RstA 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 181 
                 GGCAAAACTAAGAAATTTTCCAGGTTTTGCC 
                 UhpA DBD::RELA TAD-1, UhpA 
               
               
                   
                   
                 DBD::RELA TAD-2, UhpA 
               
               
                   
                   
                 DBD::RELA TAD-3, UhpA 
               
               
                   
                   
                 DBD::VP16 TAD-1, UhpA 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 182 
                 GGCATTTCAT 
                 YpdB DBD::RELA TAD-1, YpdB 
               
               
                   
                   
                 DBD::RELA TAD-2, YpdB 
               
               
                   
                   
                 DBD::RELA TAD-3, YpdB 
               
               
                   
                   
                 DBD::VP16 TAD-1, YpdB 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 183 
                 GCGAGTCAAAAAAACTCA 
                 ZraR DBD::RELA TAD-1, ZraR 
               
               
                   
                   
                 DBD::RELA TAD-2, ZraR 
               
               
                   
                   
                 DBD::RELA TAD-3, ZraR 
               
               
                   
                   
                 DBD::VP16 TAD-1, ZraR 
               
               
                   
                   
                 DBD::VP16 TAD-2 
               
               
                   
               
               
                 184 
                 TTCGAANNNTTCGAA 
                 HSFY1 UniProtKB-Q96LI6 
               
               
                 185 
                 rCrTTCGAAaCRTTCgAww 
                 (HSFY1_HUMAN) 
               
               
                 186 
                 rTTCGAAhsdTTCGAAy 
                   
               
               
                 187 
                 rCATTCyAAACATTCyAhw 
                   
               
               
                 188 
                 rTTCGAAysdTTCGAAy 
                   
               
               
                   
               
               
                 189 
                 ACCATATGTT 
                 OLIG3 UniProtKB-Q7RTU3 
               
               
                 190 
                 rcCATATGkr 
                 (OLIG3_HUMAN) 
               
               
                 191 
                 AvCAkmTGTT 
                   
               
               
                 192 
                 rcCATATGkT 
                   
               
               
                 193 
                 acCATATGkt 
                   
               
               
                 194 
                 am CA km TGT t 
                   
               
               
                 195 
                 
                   ACCATATGKT 
                 
                   
               
               
                 196 
                 AmCATATGby 
                   
               
               
                   
               
               
                 197 
                 sr CCA ww TG kys 
                 MSGN1 UniProtKB-A6NI15 
               
               
                 198 
                 brc CA ww TG kyv 
                 (MSGN1_HUMAN) 
               
               
                   
               
            
           
         
       
     
     In some embodiments, the regulatory component comprises a transcription factor response element. The term “transcription factor response element” refers to a DNA sequence that is bound and recognized by a transcription factor. As used herein, the term “transcription factor” refers to a protein that is not encoded on the contiguous polynucleic acid that modulates gene transcription. In some embodiments, a transcription factor is a transcription activator (i.e., increases transcription). In other embodiments, a transcription factor is a transcription inhibitor (i.e., inhibits transcription). In some embodiments, a transcription factor is an endogenous transcription factor of a cell. 
     In some embodiments, the transcription factor response element is engineered to bind to directly, or be affected indirectly, by one or more of the following transcription factors: ABL1, CEBPA, ERCC3, HIST1H2BE, MDM4, PAX7, SMARCA4, TFPT, AFF1, CHD1, ERCC6, HIST1H2BG, MED12, PAX8, SMARCB1, THRAP3, AFF3, CHD2, ERF, HLF, MEF2B, PBX1, SMARCD1, TLX1, AFF4, CHD4, ERG, HMGA1, MEF2C, PEG3, SMARCE1, TLX3, APC, CHD5, ESPL1, HMGA2, MEN1, PER1, SMURF2, TNFAIP3, AR, CHD7, ESR1, HOXA11, MITF, PHF3, SOX2, SOX4, TP53, ARID1A, CIC, ETS1, HOXA13, MKL1, PHF6, SOX5, TRIM24, ARID1B, CIITA, ETV1, HOXA7, MLLT1, PHOX2B, SOX9, TRIM33, ARID3B, CNOT3, ETV4, HOXA9, MLLT10, PLAG1, SRCAP, TRIP11, ARID5B, CREB1, ETV5, HOXC11, MLLT3, PML, SS18L1, TRPS1, ARNT, CREB3L1, ETV6, HOXC13, MLLT6, PMS1, SSB, TRRAP, ARNT2, CREBBP, EWSR1, HOXD11, MYB, PNN, SSX1, TSC22D1, ASB15, CRTC1, EYA4, HOXD13, MYBL1, MYBL2, POU2AF1, SSX2, TSHZ3, ASXL1, CSDE1, EZH2, ID3, MYC, POU2F2, SSX4, VHL, ATF1, CTCF, FEV, IRF2, MYCN, POU5F1, STAT3, WHSC1, ATF7IP, CTNNB1, FLI1, IRF4, MYOD1, PPARG, STAT4, WHSC1L1, ATM, DACH1, FOXA1, IRF6, NCOA1, PRDM1, STAT5B, WT1, ATRX, DACH2, FOXE1, IRF8, NCOA2, PRDM16, STAT6, WWP1, BAZ2B, DAXX, FOXL2, IRX6, NCOA4, PRDM9, SUFU, WWTR1, BCL11A, DDB2, FOXP1, JUN, NCOR1, PRRX1, SUZ12, XBP1, BCL11B, DDIT3, FOXQ1, KHDRBS2, NCOR2, PSIP1, TAF1, XPC, BCL3, DDX5, FUBP1, KHSRP, NEUROG2, RARA, TAF15, ZBTB16, BCL6, DEK, FUS, KLF2, NFE2L2, RB1, TAL1, ZBTB20, BCLAF1, DIP2C, FXR1, KLF4, NFE2L3, RBM15, TAL2, ZFP36L1, BCOR, DNMT1, GATA1, KLF5, NFIB, RBMX, TBX18, ZFX, BRCA1, DNMT3A, GATA2, KLF6, NFKB2, REL, TBX22, ZHX2, BRCA2, DOT1L, GATA3, LDB1, NFKBIA, RUNX1, TBX3, ZIC3, BRD7, EED, GLI3, LMO1, NONO, RUNX1T1, TCEA1, ZIM2, BRD8, EGR2, GTF2I, LMO2, NOTCH2, RXRA, TCEB1, ZNF208, BRIP1, ELAVL2, HDAC9, LMX1A, NOTCH3, SALL3, TCERG1, ZNF226, BRPF3, ELF3, HEY1, LYL1, NPM1, SATB2, TCF12, ZNF331, BTG1, ELF4, HIST1H1B, LZTR1, NR3C2, SETBP1, TCF3, ZNF384, BTG2, ELK4, HIST1H1C, MAF, NR4A3, SFPQ, TCF7L2, ZNF469, CBFA2T3, ELL, HIST1H1D, MAFA, NSD1, SIN3A, TFAP2D, ZNF595, CBFB, EP300, HIST1H1E, MAFB, OLIG2, SMAD2, TFDP1, ZNF638, CDX2, EPC1, HIST1H2BC, MAML1, PAX3, SMAD4, TFE3, CDX4, ERCC2, HIST1H2BD, MAX, PAX5, SMARCA1, and TFEB. 
     The “transcription factor response element” can comprise a minimal DNA sequence that is bound and recognized by a transcription factor. In some embodiments the transcription factor response element comprises more than one copy (i.e., repeats) of a minimal DNA sequence that is bound and recognized by a transcription factor. In some embodiments, a transcription factor response element comprises at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 repeats of a minimal DNA sequence that is bound and recognized by a transcription factor. In some embodiments the repeats are tandem repeats. In some embodiments, the transcription factor response element comprises a combination of minimal DNA sequences. In some embodiments, minimal DNA sequences are interspersed with spacer sequences. In some embodiments, a spacer sequence is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or greater than 20 nucleotides in length. In some embodiments, the transactivator response element comprises deviations from the minimal DNA sequence, or is flanked by additional DNA sequence, while still being able to bind a transactivator protein. In some embodiments, different transactivator response elements can be placed next to each other, while all being able to bind to the same transactivator protein. 
     In some embodiments, the transcription factor response element is unique (i.e., the contiguous polynucleic acid includes only one copy of the transcription factor response element). In other embodiments, the transcription factor response element is not unique. In some embodiments, a transcription factor that binds to the transcription factor response element activates expression of the RNA to which it is operably linked. In other embodiments, a transcription factor that binds to the transcription factor response element inhibits expression of the RNA to which it is operably linked. 
     In some embodiments, the regulatory component comprises at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, or at least 10 different transcription factor response elements, each bound by a different transcription factor. In some embodiments, the regulatory component comprises 2, 3, 4, 5, 6, 7, 8, 9, or 10 different transcription factor response elements, each bound by a different transcription factor. 
     Exemplary transcription factor response elements are listed in TABLE 4. In some embodiments, a transcription factor response element consists of a nucleic acid sequence listed in TABLE 4 or a nucleic acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identity to a nucleic acid sequence listed in TABLE 4. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Exemplary transcription factor response elements. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 Input 
               
               
                 Name 
                 Sensor 
                 Response Element Sequence 
                 TFs/Pathways 
               
               
                   
               
               
                 199 
                 TCF/LEF 1x 
                 AGATCAAAGGGGGTA 
                 ICF/LEF, Beta 
               
               
                   
                   
                   
                 Catenin, WNT 
               
               
                   
                   
                   
                 Pathway Activation 
               
               
                   
               
               
                 200 
                 TCF/LEF 3x 
                 AGATCAAAGGGGGTAAGATCAAAG 
                 TCF/LEF, Beta 
               
               
                   
                   
                 GGGGTAAGATCAAAGGGGGTA 
                 Catenin, WNT 
               
               
                   
                   
                   
                 Pathway Activation 
               
               
                   
               
               
                 201 
                 TCF/LEF 6x 
                 AGATCAAAGGGGGTAAGATCAAAG 
                 TCF/LEF, Beta 
               
               
                   
                   
                 GGGGTAAGATCAAAGGGGGTAAGA 
                 Catenin, WNT 
               
               
                   
                   
                 TCAAAGGGGGTAAGATCAAAGGGG 
                 Pathway Activation 
               
               
                   
                   
                 GTAAGATCAAAGGGGGTA 
                   
               
               
                   
               
               
                 202 
                 Myc 1x 
                 CGCGCCGACCACGTGGTCCA 
                 Myc 
               
               
                   
               
               
                 203 
                 Myc 2x 
                 CGCGCCGACCACGTGGTCGACCAC 
                 Myc 
               
               
                   
                   
                 GTGGTCCA 
                   
               
               
                   
               
               
                 204 
                 Myc 3x 
                 CGCGCCGACCACGTGGTCGACCAC 
                 Myc 
               
               
                   
                   
                 GTGGTCGACCACGTGGTCCA 
                   
               
               
                   
               
               
                 205 
                 HIF-1A 1x 
                 GACCTTGAGTACGTGCGTCTCTGCA 
                 HIF-1 Alpha, 
               
               
                   
                   
                 CGTATG 
                 Hypoxia Response 
               
               
                   
               
               
                 206 
                 HIF-1A2x 
                 GACCTTGAGTACGTGCGTCTCTGCA 
                 HIF-1 Alpha, 
               
               
                   
                   
                 CGTATGGACCTTGAGTACGTGCGTC 
                 Hypoxia Response 
               
               
                   
                   
                 TCTGCACGTATG 
                   
               
               
                   
               
               
                 207 
                 HIF-1A3x 
                 GACCTTGAGTACGTGCGTCTCTGCA 
                 HIF-1 Alpha, 
               
               
                   
                   
                 CGTATGGACCTTGAGTACGTGCGTC 
                 Hypoxia Response 
               
               
                   
                   
                 TCTGCACGTATGGACCTTGAGTACG 
                   
               
               
                   
                   
                 TGCGTCTCTGCACGTATG 
                   
               
               
                   
               
               
                 208 
                 3x FOXM1 
                 TGTTTATTGTTTATTGTTTAT 
                 FOXM1 
               
               
                   
                 Vitro 
                   
                   
               
               
                   
               
               
                 209 
                 6x FOXM1 
                 TGTTTATTGTTTATTGTTTATTGTTT 
                 FOXM1 
               
               
                   
                 Vitro 
                 ATTGTTTATTGTTTAT 
                   
               
               
                   
               
               
                 210 
                 3x FOXM1 
                 GCAAAGCAAACAGCAAAGCAAACA 
                 FOXM1 
               
               
                   
                 ChipSeq Fwd 
                 GCAAAGCAAACA 
                   
               
               
                   
               
               
                 211 
                 6x FOXM1 
                 GCAAAGCAAACAGCAAAGCAAACA 
                 FOXM1 
               
               
                   
                 ChipSeq Fwd 
                 GCAAAGCAAACAGCAAAGCAAACA 
                   
               
               
                   
                   
                 GCAAAGCAAACAGCAAAGCAAACA 
                   
               
               
                   
               
               
                 212 
                 3x FOXM1 
                 TGTTTGCTTTGCTGTTTGCTTTGCTG 
                 FOXM1 
               
               
                   
                 ChipSeq Rev 
                 TTTGCTTTGC 
                   
               
               
                   
               
               
                 213 
                 6x FOXM1 
                 TGTTTGCTTTGCTGTTTGCTTTGCTG 
                 FOXM1 
               
               
                   
                 ChipSeq Rev 
                 TTTGCTTTGCTGTTTGCTTTGCTGTT 
                   
               
               
                   
                   
                 TGCTTTGCTGTTTGCTTTGC 
                   
               
               
                   
               
               
                 214 
                 8x Gli2 (3,4) 
                 GAACACCCAGAACACCCAGAACAC 
                 Gli2, Gli1, SHH 
               
               
                   
                   
                 CCAGAACACCCAGAACACCCAGAA 
                 Pathway Activation 
               
               
                   
                   
                 CACCCAGAACACCCAGAACACCCA 
                   
               
               
                   
               
               
                 215 
                 6x Gli2 (3,4) 
                 GAACACCCAGAACACCCAGAACAC 
                 Gli2, Gli1, SHH 
               
               
                   
                   
                 CCAGAACACCCAGAACACCCAGAA 
                 Pathway Activation 
               
               
                   
                   
                 CACCCA 
                   
               
               
                   
               
               
                 216 
                 HNF1 1x 
                 AGTTAATAATTTAAC 
                 HNF1A, HNF1B 
               
               
                   
               
               
                 217 
                 HNF1 2x 
                 AGTTAATAATTTAACAGTTAATAAT 
                 HNF1A, HNF1B 
               
               
                   
                   
                 TTAAC 
                   
               
               
                   
               
               
                 218 
                 HNF1 3x 
                 AGTTAATAATTTAACAGTTAATAAT 
                 HNF1A, HNF1B 
               
               
                   
                   
                 TTAAC AGTTAATAATTTAAC 
                   
               
               
                   
               
               
                 219 
                 HNF1 4x 
                 AGTTAATAATTTAACAGTTAATAAT 
                 HNF1A, HNF1B 
               
               
                   
                   
                 TTAACAGTTAATAATTTAACAGTTA 
                   
               
               
                   
                   
                 ATAATTTAAC 
                   
               
               
                   
               
               
                 220 
                 2x SOX9/10 
                 CTACACAAAGCCCTCTGTGTAAGAC 
                 SOX9., SOX10, 
               
               
                   
                 C-C’ 
                 TACACAAAGCCCTCTGTGTAAGA 
                 SOX6, SOX8 Low 
               
               
                   
                   
                   
                 affinity: SOX4, 
               
               
                   
                   
                   
                 SOX2, SOX21 
               
               
                   
                   
                   
                 (Noon cooperative) 
               
               
                   
               
               
                 221 
                 3x SOX9/10 
                 CTACACAAAGCCCTCTGTGTAAGAC 
                 SOX9., SOX10, 
               
               
                   
                 C-C’ 
                 TACACAAAGCCCTCTGTGTAAGACT 
                 SOX6, SOX8 
               
               
                   
                   
                 ACACAAAGCCCTCTGTGTAAGA 
                 Low affinity: SOX4, 
               
               
                   
                   
                   
                 SOX2, SOX21 
               
               
                   
                   
                   
                 (Noon cooperative) 
               
               
                   
               
               
                 222 
                 2x SOX9/10 
                 CTACACAAAGCCCTCTTTGTGAGAC 
                 SOX9., SOX10, 
               
               
                   
                 C-C 
                 TACACAAAGCCCTCTTTGTGAGA 
                 SOX6, SOX8 
               
               
                   
                   
                   
                 SOX4, SOX2, 
               
               
                   
                   
                   
                 SOX21 
               
               
                   
               
               
                 223 
                 3x SOX9/10 
                 CTACACAAAGCCCTCTTTGTGAGAC 
                 SOX9., SOX10, 
               
               
                   
                 C-C 
                 TACACAAAGCCCTCTTTGTGAGACT 
                 SOX6, SOX8 
               
               
                   
                   
                 ACACAAAGCCCTCTTTGTGAGA 
                 SOX4, SOX2, 
               
               
                   
                   
                   
                 SOX21 
               
               
                   
               
               
                 224 
                 3X Sox 4/9 
                 CCATTGTTCT CCATTGTTCT 
                 SOX4 SOX9 
               
               
                   
                   
                 CCATTGTTCT 
                   
               
               
                   
               
               
                 225 
                 6X Sox 4/9 
                 CCATTGTTCTCCATTGTTCTCCATTG 
                 SOX4 SOX9 
               
               
                   
                   
                 TTCTCCATTGTTCTCCATTGTTCTCC 
                   
               
               
                   
                   
                 ATTGTTCT 
                   
               
               
                   
               
               
                 226 
                 6X Sox 4/11 
                 AACAAAGAACAAAGAACAAAGAAC 
                 SOXC Family 
               
               
                   
                   
                 AAAG 
                   
               
               
                   
               
               
                 227 
                 3x MYBL2 
                 AACCGTTAAACGGTTAACCGTTAAA 
                 MYBL2 
               
               
                   
                   
                 CGGTTAACCGTTAAACGGTT 
                   
               
               
                   
               
               
                 228 
                 MYBL2- 
                 AGAGATATTTAGTGAATCAGCAAGT 
                 MYBL2 MuvB 
               
               
                   
                 CCNB1 
                 GGAACCAAAAAGACTTGAGGACTG 
                 FoxM1 
               
               
                   
                   
                 ATTGGATGAGGAGAGGTTAG 
                   
               
               
                   
               
               
                 229 
                 2x MYBL2- 
                 AGAGATATTTAGTGAATCAGCAAGT 
                 MYBL2 MuvB 
               
               
                   
                 CCNB1 
                 GGAACCAAAAAGACTTGAGGACTG 
                 FoxM1 
               
               
                   
                   
                 ATTGGATGAGGAGAGGTTAGAGAG 
                   
               
               
                   
                   
                 ATATTTAGTGAATCAGCAAGTGGAA 
                   
               
               
                   
                   
                 CCAAAAAGACTTGAGGACTGATTG 
                   
               
               
                   
                   
                 GATGAGGAGAGGTTAG 
                   
               
               
                   
               
               
                 230 
                 MYBL2-Plk1 
                 ACTGGTGCCCTCCTCAACTCCCACC 
                 MYBL2 MuvB 
               
               
                   
                   
                 TGCATCTGGGGCCCATACTGGTTGG 
                 FoxM1 
               
               
                   
                   
                 CTCCCGCGGTGCCATGTCTGCAGTG 
                   
               
               
                   
                   
                 TGCCCCCCAGCCCCGG 
                   
               
               
                   
               
               
                 231 
                 2x MYBL2- 
                 ACTGGTGCCCTCCTCAACTCCCACC 
                 MYBL2 MuvB 
               
               
                   
                 Plkl 
                 TGCATCTGGGGCCCATACTGGTTGG 
                 FoxM1 
               
               
                   
                   
                 CTCCCGCGGTGCCATGTCTGCAGTG 
                   
               
               
                   
                   
                 TGCCCCCCAGCCCCGGACTGGTGCC 
                   
               
               
                   
                   
                 CTCCTCAACTCCCACCTGCATCTGG 
                   
               
               
                   
                   
                 GGCCCATACTGGTTGGCTCCCGCGG 
                   
               
               
                   
                   
                 TGCCATGTCTGCAGTGTGCCCCCCA 
                   
               
               
                   
                   
                 GCCCCGG 
                   
               
               
                   
               
               
                 232 
                 Myc 8x 
                 CGCGCCGACCACGTGGTCGACCAC 
                 Myc 
               
               
                   
                   
                 GTGGTCCACGCGCCGACCACGTGGT 
                   
               
               
                   
                   
                 CGACCACGTGGTCCACGCGCCGACC 
                   
               
               
                   
                   
                 ACGTGGTCGACCACGTGGTCCACGC 
                   
               
               
                   
                   
                 GCCGACCACGTGGTCGACCACGTG 
                   
               
               
                   
                   
                 GTCCA 
                   
               
               
                   
               
               
                 233 
                 Myc/USF1 4x 
                 GTCACGTGGCTCAGTCACGTGGCTC 
                 Myc USF1 
               
               
                   
                   
                 AGTCACGTGGCTCAGTCACGTGGC 
                   
               
               
                   
               
               
                 234 
                 Myc/USF1 8x 
                 GTCACGTGGCTCAGTCACGTGGCTC 
                 Myc USF1 
               
               
                   
                   
                 AGTCACGTGGCTCAGTCACGTGGCG 
                   
               
               
                   
                   
                 TCACGTGGCTCAGTCACGTGGCTCA 
                   
               
               
                   
                   
                 GTCACGTGGCTCAGTCACGTGGC 
                   
               
               
                   
               
               
                 235 
                 EBOX Myc 
                 GACCACGTGGTCGACCACGTGGTCG 
                 Myc 
               
               
                   
                 4x 
                 ACCACGTGGTCGACCACGTGGTC 
                   
               
               
                   
               
               
                 236 
                 EBOX Myc 
                 GACCACGTGGTCGACCACGTGGTCG 
                 Myc 
               
               
                   
                 8x 
                 ACCACGTGGTCGACCACGTGGTCGA 
                   
               
               
                   
                   
                 CCACGTGGTCGACCACGTGGTCGAC 
                   
               
               
                   
                   
                 CACGTGGTCGACCACGTGGTC 
                   
               
               
                   
               
               
                 237 
                 8x TCF/LEF 
                 CCTCTACCCCCTTTGATCTTACCCCC 
                 TCF/LEF, Beta 
               
               
                   
                 (Beta Catenin) 
                 TTTGATCTTACCCCCTTTGATCTTAC 
                 Catenin, WNT 
               
               
                   
                   
                 CCCCTTTGATCTTACCCCCTTTGATC 
                 Pathway Activation 
               
               
                   
                   
                 TTACCCCCTTTGATCTTACCCCCTTT 
                   
               
               
                   
                   
                 GATCTTACCCCCTTTGATCT 
               
               
                   
               
            
           
         
       
     
     In some embodiments, a regulatory component comprises a promoter element (or a promoter fragment). Exemplary promoter elements are listed in TABLE 5. In some embodiments, a promoter element consists of a nucleic acid sequence listed in TABLE 5 or a nucleic acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identity to a nucleic acid sequence listed in TABLE 5. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Exemplary promoter elements. 
               
            
           
           
               
               
               
            
               
                 Seq 
                   
                   
               
               
                 ID 
                 Name 
                 SEQUENCE 
               
               
                   
               
               
                 238 
                 AFP 0.5 
                 GGCCTGAAATAACCTCTGAAAGAGGAACTTGGTTAGGTACCTTCTGAGGCT 
               
               
                   
                 Core 
                 GAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCA 
               
               
                   
                   
                 GGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCA 
               
               
                   
                   
                 ACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAG 
               
               
                   
                   
                 CATGCATCTCAATTAGTCAGCAACCATAGTCCCACTGCAGTTTGAGGAGAA 
               
               
                   
                   
                 TATTTGTTATATTTGCAAAATAAAATAAGTTTGCAAGTTTTTTTTTTCTGCCC 
               
               
                   
                   
                 CAAAGAGCTCTGTGTCCTTGAACATAAAATACAAATAACCGCTATGCTGTT 
               
               
                   
                   
                 AATTATTGGCAAATGTCCCATTTTCAACCTAAGGAAATACCATAAAGTAAC 
               
               
                   
                   
                 AGATATACCAACAAAAGGTTACTAGTTAACAGGCATTGCCTGAAAAGAGT 
               
               
                   
                   
                 ATAAAAGAATTTCAGCATGATTTTCCATATTGTGCTTCCACCACTGCCAATA 
               
               
                   
                   
                 ACAC 
               
               
                   
               
               
                 239 
                 AFP 0.2 
                 CTGTGTCCTTGAACATAAAATACAAATAACCGCTATGCTGTTAATTATTGGC 
               
               
                   
                 Core 
                 AAATGTCCCATTTTCAACCTAAGGAAATACCATAAAGTAACAGATATACCA 
               
               
                   
                   
                 ACAAAAGGTTACTAGTTAACAGGCATTGCCTGAAAAGAGTATAAAAGAAT 
               
               
                   
                   
                 TTCAGCATGATTTTCCATATTGTGCTTCCACCACTGCCAATAACAC 
               
               
                   
               
               
                 240 
                 AFP 
                 AAATTAGTTTTGAATCTTTCTAATACCAAAGTTCAGTTTACTGTTCCATGTT 
               
               
                   
                 Enhancer 
                 GCTTCTGAGTGGCTTCACAGACTTATGAAAAAGTAAACGGAATCAGAATTA 
               
               
                   
                 +0.2 Core 
                 CATCAATGCAAAAGCATTGCTGTGAACTCTGTACTTAGGACTAAACTTTGA 
               
               
                   
                   
                 GCAATAACACATATAGATTGAGGATTGTTTGCTGTTAGTATACAAACTCTG 
               
               
                   
                   
                 GTTCAAAGCTCCTCTTTATTGCTTGTCTTGGAAAATTTGCTGTTCTTCATGGT 
               
               
                   
                   
                 TTCTCTTTTCACTGCTATCTATTTTTCTCAACCACTCACATGGCTACAATAAC 
               
               
                   
                   
                 TGTCTGCAAGCTTATGATTCCCAAATATCTATCTCTAGCCTCAATCTTGTTC 
               
               
                   
                   
                 CAGAAGATAAAAAGTAGTATTCAAATGCACATCAACGTCTCCACTTGGAGG 
               
               
                   
                   
                 GCTTAAAGACGTTTCAACATACAAACCGGGGAGTTTTGCCTGGAATGTTTC 
               
               
                   
                   
                 CTAAAATGTGTCCTGTAGCACATAGGGTCCTCTTGTTCCTTAAAATCTAATT 
               
               
                   
                   
                 ACTTTTAGCCCAGTGCTCATCCCACCTATGGGGAGATGAGAGTGAAAAGGG 
               
               
                   
                   
                 AGCCTGATTAATAATTACACTAAGTCAATAGGCATAGAGCCAGGACTGTTT 
               
               
                   
                   
                 GGGTAAACTGGTCACTTTATCTTAAACTAAATATATCCAAAACTGAACATG 
               
               
                   
                   
                 TACTTAGTTACTAAGTCTTTGACTTTATCTCATTCATACCACTCAGCTTTATC 
               
               
                   
                   
                 CAGGCCACTTATTTGACAGTATTATTGCGAAAACTTCCTAACTGGTCTCCTT 
               
               
                   
                   
                 ATCATAGTCTTATCCCCTTTTGAAACAAAAGAGACAGTTTCAAAATACAAA 
               
               
                   
                   
                 TATGATTTTTATTAGCTCCCTTTTGTTGTCTATAATAGTCCCAGAAGGAGTT 
               
               
                   
                   
                 ATAAACTCCATTTAAAAAGTCTTTGAGATGTGGCCCTTGCCAACTTTGCCAG 
               
               
                   
                   
                 GCTGTGTCCTTGAACATAAAATACAAATAACCGCTATGCTGTTAATTATTG 
               
               
                   
                   
                 GCAAATGTCCCATTTTCAACCTAAGGAAATACCATAAAGTAACAGATATAC 
               
               
                   
                   
                 CAACAAAAGGTTACTAGTTAACAGGCATTGCCTGAAAAGAGTATAAAAGA 
               
               
                   
                   
                 ATTTCAGCATGATTTTCCCAAGTTTGCTTATTTATGAAAAGTTATCGATAAT 
               
               
                   
                   
                 TTCTTTAGTTTTGTAT 
               
               
                   
               
               
                 241 
                 Midkine 
                 TCCCTGCCCACCCGCGGAAACCGCCCCAGGTGGGCCGCGCCCCCTCCCCAG 
               
               
                   
                 600 
                 CAGCCAGCAGGGCGCCAGGGCTGAGCCGGCCGTGGAGGGGAGCGGGTCCC 
               
               
                   
                   
                 GCGGGTTATACAGGCGCCGGGGCTCCGCGGCAGGCAAGAGAAGCTGAGGC 
               
               
                   
                   
                 CTGAGAACGGCCCGGGCCTTGGCGTACGGCAGGGGACGACCTGGGATGGG 
               
               
                   
                   
                 GGCAGCGGGCGGCGGCGCAGGGAGTGGGCCGGGGGCCGGTGTGCGCGGGC 
               
               
                   
                   
                 GGGACGGGGCCCGGGGTCGGGAGACCACCGCTCGGAAGATGGGGCCGGGA 
               
               
                   
                   
                 GAGGCCGCCGTCGCAGCGCAGAGGGCACCGGCGGGGAGACGCGAGGACGC 
               
               
                   
                   
                 GGGGCCGGGAACACGGACGCCGGAGTAGAAGCGCGGGGGGCGCGGGCTG 
               
               
                   
                   
                 GAGCGGGGGCGGGGACGCCGGGGTCGGGGGCGGTGCGGGTTTGAGGGGAG 
               
               
                   
                   
                 GGGGCGGGGCGGGTCCTTCCCTGGGGGGGTGGGGAGAGGGGGCGGGGGCC 
               
               
                   
                   
                 CATGTGACCGGCTCAGACCGGTTCTGGAGACAAAAGGGGCCGCGGCGGCC 
               
               
                   
                   
                 GGAGCGGGACGGGCCCGGCGCGGGAGGGAGCGAAGCAGCGCGGGCAGCG 
               
               
                   
                   
                 AGCGAGTGAG 
               
               
                   
               
               
                 242 
                 Midkine 
                 ACCACCGCTCGGAAGATGGGGCCGGGAGAGGCCGCCGTCGCAGCGCAGAG 
               
               
                   
                 300 
                 GGCACCGGCGGGGAGACGCGAGGACGCGGGGCCGGGAACACGGACGCCG 
               
               
                   
                   
                 GAGTAGAAGCGCGGGGGGCGCGGGCTGGAGCGGGGGCGGGGACGCCGGG 
               
               
                   
                   
                 GTCGGGGGCGGTGCGGGTTTGAGGGGAGGGGGCGGGGCGGGTCCTTCCCT 
               
               
                   
                   
                 GGGGGGGTGGGGAGAGGGGGCGGGGGCCCATGTGACCGGCTCAGACCGGT 
               
               
                   
                   
                 TCTGGAGACAAAAGGGGCCGCGGCGGCCGGAGCGGGACGGGCCCGGCGCG 
               
               
                   
                   
                 GGAGGGAGCGAAGCAGCGCGG 
               
               
                   
               
               
                 243 
                 Midkine 
                 CCGCGGCGGCCGGAGCGGGACGGGCCCGGCGCGGGAGGGAGCGAAGCAG 
               
               
                   
                 70 
                 CGCGGGCAGCGAGCGAGTGAG 
               
               
                   
               
               
                 244 
                 Glypican-3 
                 GGAGTCTCACTCTGTCGCCCAAGCTGGAGTGCAGTAGTGCGATCTCAGCTC 
               
               
                   
                 1.5 
                 ACTGCAACCTCTGCCCTCTGAGTTCAAGTGATTCTCCTGCCTCAGCCTCCCG 
               
               
                   
                   
                 AGTAGCTGGGATTACAGGCGCCTGCCACCGCGCCCAGCTAATTTTTTGTATT 
               
               
                   
                   
                 TTTGGTAGAGACGGGGTTTCACCATCTTGGCCAGGCTGGTCTTGAACTCCTG 
               
               
                   
                   
                 ACCTCATGATCCACCCGCCTCGGCTTCCCAAAGTGCTGGGATTACAGGCGT 
               
               
                   
                   
                 GAGCCACCGTGCCTGGCCTAAAGAACTGGATTTCTAATGGTGAAATCTAAG 
               
               
                   
                   
                 CAGGAGAGGTGGGATTTGGGTGTAGGATACCTTTCAAATAGCCTTCTACTC 
               
               
                   
                   
                 CATCTATGAAATAGGCTAGCTTTGGCTCAGTAAATTTGCTGTGTAATGATTT 
               
               
                   
                   
                 TCTAATGAGTTAGGCTGGCTTTAAGCCCCTGGTTATTTCGTTGTAACCAGTT 
               
               
                   
                   
                 AGGCTTTGCCTCTTGAAGGGCCACCTGGGACrGTCGTGCAGTAGATTTTCTT 
               
               
                   
                   
                 TTAACGCCCCAGAATCAGGTGCTTTCTCTGACTTTGTGTGGCTCTACTGAAT 
               
               
                   
                   
                 CAAATCTAGCAAGCCACAGAGGCTTTCAGACTTTTAAGATACAATATTCAA 
               
               
                   
                   
                 AGGTGAGGCAGGCTGTGAAAAGCCCAGCGGTCCCTGGCTGTCCCTGAACGC 
               
               
                   
                   
                 GACTATTTGCAGGTTGGCTTTGAGAACCCGGTCAGAGCTGCGTAGGAAAA 
               
               
                   
                   
                 CGGTTCCCGGGAAGCTCCTCAGAGAGTAGAATGAGGAGGTGGATTTTGTGT 
               
               
                   
                   
                 GAAGGAACACCTTGTGTGGCTCTGGTGGCCAGGAAAGAGCTGGCACAAGC 
               
               
                   
                   
                 TGAAAGAAGGCCTGTGGCGAAGCGGAGGGGGACCTAAGTCAGGGACCCCC 
               
               
                   
                   
                 ACCTGCCCCCAGGAAGGATGAAAAGGAGACAAAAATCCTAAAGGGAAAAG 
               
               
                   
                   
                 CCCTCCAGGCTGTAGGCCAATGAGCGGCGGGAAGGAGGAGTGAGGCTGGG 
               
               
                   
                   
                 GAACTTCTCCCAGAGCCAGTCAGAGCGGACGGCTGCTGGGAAGCCAATCA 
               
               
                   
                   
                 GCGCGCTCGAGCCTGCAGCCCCTCTGCAGTAGTTATGCCAGAGCGCCCTGT 
               
               
                   
                   
                 GTAGAGCGGCTGCGAGCGGGCAGCTGGGCTCGGCTGCCGGGAGCCACCGC 
               
               
                   
                   
                 GCGGGCTCCGCACCCTCCTCTCGCACTGCCTTCGCCCGGTCCCCGCGCCGCG 
               
               
                   
                   
                 GTGCCCCAGTGGCCCCCGCCGCGCTCCACGCCGCGCCCCCGCACCCCGCCG 
               
               
                   
                   
                 GCTACCGGCCGCACAACCGCCACCGCCCCCTGGCCGCGCGGCTCGCCTCGC 
               
               
                   
                   
                 CCCGCCCCGTCCCTCCTCGCCCCGCCCCACCCCAGTCAGCCCCGCCCTGCCC 
               
               
                   
                   
                 CGCGCCGCCAAGCGGTTCCCGCCCTCGCCCAGCGCCCAGGTAGCTGCGAGG 
               
               
                   
                   
                 AAACTTTTGCAGCGGCTGGGTAGCAGCACGTCTCTTGCTCCTCAGGGCCAC 
               
               
                   
                   
                 TGCCAGGCTTGCCGAGTCCTGGGACTGCTCTCGCTCCGGCTGCCACTCTCCC 
               
               
                   
                   
                 GCGCTCTCCTAGCTCCCTGCGAAGCAGG 
               
               
                   
               
               
                 245 
                 Glypican-3 
                 GGAGAGGTGGGATTTGGGTGTAGGATACCTTTCAAATAGCCTTCTACTCCA 
               
               
                   
                 1.2 
                 TCTATGAAATAGGCTAGCTTTGGCTCAGTAAATTTGCTGTGTAATGATTTTC 
               
               
                   
                   
                 TAATGAGTTAGGCTGGCTTTAAGCCCCTGGTTATTTCGTTGTAACCAGTTAG 
               
               
                   
                   
                 GCTTTGCCTCTTGAAGGGCCACCTGGGACTGTCGTGCAGTAGATTTTCTTTT 
               
               
                   
                   
                 AACGCCCCAGAATCAGGTGCTTTCTCTGACTTTGTGTGGCTCTACTGAATCA 
               
               
                   
                   
                 AATCTAGCAAGCCACAGAGGCTTTCAGACTTTTAAGATACAATATTCAAAG 
               
               
                   
                   
                 GTGAGGCAGGCTGTGAAAAGCCCAGCGGTCCCTGGCTGTCCCTGAACGCGA 
               
               
                   
                   
                 CTATTTGCAGGTTGGCTTTGAGAACCCGGTCAGAGCTGCGTTAGGAAAACG 
               
               
                   
                   
                 GTTCCCGGGAAGCTCCTCAGAGAGTAGAATGAGGAGGTGGATTTTGTGTGA 
               
               
                   
                   
                 AGGAACACCTTGTGTGGCTCTGGTGGCCAGGAAAGAGCTGGCACAAGCTG 
               
               
                   
                   
                 AAAGAAGGCCTGTGGCGAAGCGGAGGGGGACCTAAGTCAGGGACCCCCAC 
               
               
                   
                   
                 CTGCCCCCAGGAAGGATGAAAAGGAGACAAAAATCCTAAAGGGAAAAGCC 
               
               
                   
                   
                 CTCCAGGCTGTAGGCCAATGAGCGGCGGGAAGGAGGAGTGAGGCTGGGGA 
               
               
                   
                   
                 ACTTCTCCCAGAGCCAGTCAGAGCGGACGGCTGCTGGGAAGCCAATCAGC 
               
               
                   
                   
                 GCGCTCGAGCCTGCAGCCCCTCTGCAGTAGTTATGCCAGAGCGCCCTGTGT 
               
               
                   
                   
                 AGAGCGGCTGCGAGCGGGCAGCTGGGCTCGGCTGCCGGGAGCCACCGCGC 
               
               
                   
                   
                 GGGCTCCGCACCCTCCTCTCGCACTGCCTTCGCCCGGTCCCCGCGCCGCGGT 
               
               
                   
                   
                 GCCCCAGTGGCCCCCGCCGCGCTCCACGCCGCGCCCCCGCACCCCGCCGGC 
               
               
                   
                   
                 TACCGGCCGCACAACCGCCACCGCCCCCTGGCCGCGCGGCTCGCCTCGCCC 
               
               
                   
                   
                 CGCCCCGTCCCTCCTCGCCCCGCCCCACCCCAGTCAGCCCCGCCCTGCCCCG 
               
               
                   
                   
                 CGCCGCCAAGCGGTTCCCGCCCTCGCCCAGCGCCCAGGTAGCTGCGAGGAA 
               
               
                   
                   
                 ACTTTTGCAGCGGCTGGGTAGCAGCACGTCTCTTGCTCCTCAGGGCCACTG 
               
               
                   
                   
                 CCAGGCTTGCCGAGTCCTGGGACTGCTCTCGCTCCGGCTGCCACTCTCCCGC 
               
               
                   
                   
                 GCTCTCCTAGCTCCCTGCGAAGCAGG 
               
               
                   
               
               
                 246 
                 Glypican-3 
                 AAAGGGAAAAGCCCTCCAGGCTGTAGGCCAATGAGCGGCGGGAAGGAGGA 
               
               
                   
                 0.6 
                 GTGAGGCTGGGGAACTTCTCCCAGAGCCAGTCAGAGCGGACGGCTGCTGG 
               
               
                   
                   
                 GAAGCCAATCAGCGCGCTCGAGCCTGCAGCCCCTCTGCAGTAGTTATGCCA 
               
               
                   
                   
                 GAGCGCCCTGTGTAGAGCGGCTGCGAGCGGGCAGCTGGGCTCGGCTGCCG 
               
               
                   
                   
                 GGAGCCACCGCGCGGGCTCCGCACCCTCCTCTCGCACTGCCTTCGCCCGGT 
               
               
                   
                   
                 CCCCGCGCCGCGGTGCCCCAGTGGCCCCCGCCGCGCTCCACGCCGCGCCCC 
               
               
                   
                   
                 CGCACCCCGCCGGCTACCGGCCGCACAACCGCCACCGCCCCCTGGCCGCGC 
               
               
                   
                   
                 GGCTCGCCTCGCCCCGCCCCGTCCCTCCTCGCCCCGCCCCACCCCAGTCAGC 
               
               
                   
                   
                 CCCGCCCTGCCCCGCGCCGCCAAGCGGTTCCCGCCCTCGCCCAGCGCCCAG 
               
               
                   
                   
                 GTAGCTGCGAGGAAACTTTTGCAGCGGCTGGGTAGCAGCACGTCTCTTGCT 
               
               
                   
                   
                 CCTCAGGGCCACTGCCAGGCTTGCCGAGTCCTGGGACTGCTCTCGCTCCGG 
               
               
                   
                   
                 CTGCCACTCTCCCGCGCTCTCCTAGCTCCCTGCGAAGCAGG 
               
               
                   
               
               
                 247 
                 Glypican-3 
                 CCCCGCACCCCGCCGGCTACCGGCCGCACAACCGCCACCGCCCCCTGGCCG 
               
               
                   
                 0.3 
                 CGCGGCTCGCCTCGCCCCGCCCCGTCCCTCCTCGCCCCGCCCCACCCCAGTC 
               
               
                   
                   
                 AGCCCCGCCCTGCCCCGCGCCGCCAAGCGGTTCCCGCCCTCGCCCAGCGCC 
               
               
                   
                   
                 CAGGTAGCTGCGAGGAAACTTTTGCAGCGGCTGGGTAGCAGCACGTCTCTT 
               
               
                   
                   
                 GCTCCTCAGGGCCACTGCCAGGCTTGCCGAGTCCTGGGACTGCTCTCGCTC 
               
               
                   
                   
                 CGGCTGCCACTCTCCCGCGCTCTCCTAGCTCCCTGCGAAGCAGG 
               
               
                   
               
               
                 248 
                 Glypican-3 
                 GTCAGCCCCGCCCTGCCCCGCGCCGCCAAGCGGTTCCCGCCCTCGCCCAGC 
               
               
                   
                 0.2 
                 GCCCAGGTAGCTGCGAGGAAACTTTTGCAGCGGCTGGGTAGCAGCACGTCT 
               
               
                   
                   
                 CTTGCTCCTCAGGGCCACTGCCAGGCTTGCCGAGTCCTGGGACTGCTCTCGC 
               
               
                   
                   
                 TCCGGCTGCCACTCTCCCGCGCTCTCCTAGCTCCCTGCGAAGCAGG 
               
               
                   
               
               
                 249 
                 Glypican-3 
                 CGCCCAGGTAGCTGCGAGGAAACTTTTGCAGCGGCTGGGTAGCAGCACGTC 
               
               
                   
                 150 bp 
                 TCTTGCTCCTCAGGGCCACTGCCAGGCTTGCCGAGTCCTGGGACTGCTCTCG 
               
               
                   
                   
                 CTCCGGCTGCCACTCTCCCGCGCTCTCCTAGCTCCCTGCGAAGCAGG 
               
               
                   
               
               
                 250 
                 hTERT 
                 TGGCCCCTCCCTCGGGTTACCCCACAGCCTAGGCCGATTCGACCTCTCTCCG 
               
               
                   
                 455 
                 CTGGGGCCCTCGCTGGCGTCCCTGCACCCTGGGAGCGCGAGCGGCGCGCGG 
               
               
                   
                   
                 GCGGGGAAGCGCGGCCCAGACCCCCGGGTCCGCCCGGAGCAGCTGCGCTG 
               
               
                   
                   
                 TCGGGGCCAGGCCGGGCTCCCAGTGGATTCGCGGGCACAGACGCCCAGGA 
               
               
                   
                   
                 CCGCGCTTCCCACGTGGCGGAGGGACTGGGGACCCGGGCACCCGTCCTGCC 
               
               
                   
                   
                 CCTTCACCTTCCAGCTCCGCCTCCTCCGCGCGGACCCCGCCCCGTCCCGACC 
               
               
                   
                   
                 CCTCCCGGGTCCCCGGCCCAGCCCCCTCCGGGCCCTCCCAGCCCCTCCCCTT 
               
               
                   
                   
                 CCTTTCCGCGGCCCCGCCCTCTCCTCGCGGCGCGAGTTTCAGGCAGCGCTGC 
               
               
                   
                   
                 GTCCTGCTGCGCACGTGGGAAGCCCTGGCCCCGGCCACCCCCGCG 
               
               
                   
               
               
                 251 
                 hTERT 
                 CCAGGACCGCGCTTCCCACGTGGCGGAGGGACTGGGGACCCGGGCACCCG 
               
               
                   
                 258 
                 TCCTGCCCCTTCACCTTCCAGCTCCGCCTCCTCCGCGCGGACCCCGCCCCGT 
               
               
                   
                   
                 CCCGACCCCTCCCGGGTCCCCGGCCCAGCCCCCTCCGGGCCCTCCCAGCCC 
               
               
                   
                   
                 CTCCCCTTCCTTTCCGCGGCCCCGCCCTCTCCTCGCGGCGCGAGTTTCAGGC 
               
               
                   
                   
                 AGCGCTGCGTCCTGCTGCGCACGTGGGAAGCCCTGGCCCCGGCCACCCCCG 
               
               
                   
                   
                 CG 
               
               
                   
               
               
                 252 
                 hTERT 
                 CGTCCCGACCCCTCCCGGGTCCCCGGCCCAGCCCCCTCCGGGCCCTCCCAG 
               
               
                   
                 159 
                 CCCCTCCCCTTCCTTrCCGCGGCCCCGCCCrCTCCTCGCGGCGCGAGTTTCA 
               
               
                   
                   
                 GGCAGCGCTGCGTCCTGCTGCGCACGTGGGAAGCCCTGGCCCCGGCCACCC 
               
               
                   
                   
                 CCGCG 
               
               
                   
               
               
                 253 
                 hTERT 
                 CCCCTCCCCTTCCTTTCCGCGGCCCCGCCCTCTCCTCGCGGCGCGAGTTTCA 
               
               
                   
                 108 
                 GGCAGCGCTGCGTCCTGCTGCGCACGTGGGAAGCCCTGGCCCCGGCCACCC 
               
               
                   
                   
                 CCGCG 
               
               
                   
               
               
                 254 
                 hTERT 83 
                 CCCGGGTCCCCGGCCCAGCCCCCTCCGGGCCCTCCCAGCCCCTCCCCTTCCI 
               
               
                   
                   
                 TTCCGCGGCCCCGCCCTCTCCTCGCGGCGCG 
               
               
                   
               
               
                 255 
                 Survivin 
                 CCATAGAACCAGAGAAGTGAGTGGATGTGATGCCCAGCTCCAGAAGTGAC 
               
               
                   
                 976 
                 TCCAGAACACCCTGTTCCAAAGCAGAGGACACACTGATTTTTTTTTTAATAG 
               
               
                   
                 (BIRC5) 
                 GCTGCAGGACTTACTGTTGGTGGGACGCCCTGCTTTGCGAAGGGAAAGGAG 
               
               
                   
                   
                 GAGTTTGCCCTGAGCACAGGCCCCCACCCTCCACTGGGCTTTCCCCAGCTCC 
               
               
                   
                   
                 CTTGTCTTCTTATCACGGTAGTGGCCCAGTCCCTGGCCCCTGACTCCAOAAG 
               
               
                   
                   
                 GTGGCCCTCCTGGAAACCCAGGTCGTGCAGTCAACGATGTACTCGCCGGGA 
               
               
                   
                   
                 CAGCGATGTCTGCTGCACTCCATCCCTCCCCTGTTCATTTGTCCTTCATGCC 
               
               
                   
                   
                 CGTCTGGAGTAGATGCTTTTTGCAGAGGTGGCACCCTGTAAAGCTCTCCTGT 
               
               
                   
                   
                 CTGACTTTTTTTTTTTTTTTAGACTGAGTTTTGCTCTTGTTGCCTAGGCTGGA 
               
               
                   
                   
                 GTGCAATGGCACAATCTCAGCTCACTGCACCCTCTGCCTCCCGGGTTCAAG 
               
               
                   
                   
                 CGATTCTCCTGCCTCAGCCTCCCGAGTAGTTGGGATTACAGGCATGCACCA 
               
               
                   
                   
                 CCACGCCCAGCTAATTTTTGTATTTTTAGTAGAGACAAGGTTTCACCGTGAT 
               
               
                   
                   
                 GGCCAGGCTGGTCTTGAACTCCAGGACTCAAGTGATGCTCCTGCCTAGGCC 
               
               
                   
                   
                 TCTCAAAGTGTTGGGATTACAGGCGTGAGCCACTGCACCCGGCCTGCACGC 
               
               
                   
                   
                 GTTCTTTGAAAGCAGTCGAGGGGGCGCTAGGTGTGGGCAGGGACGAGCTG 
               
               
                   
                   
                 GCGCGGCGTCGCTGGGTGCACCGCGACCACGGGCAGAGCCACGCGGCGGG 
               
               
                   
                   
                 AGGACTACAACTCCCGGCACACCCCGCGCCGCCCCGCCTCTACTCCCAGAA 
               
               
                   
                   
                 GGCCGCGGGGGGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATGCCCC 
               
               
                   
                   
                 GCGGCGCGCCATTAACCGCCAGATTTGAATCGCGGGACCCGTTGGCAGAGG 
               
               
                   
                   
                 TGG 
               
               
                   
               
               
                 256 
                 Survivin 
                 CAATCTCAGCTCACTGCACCCTCTGCCTCCCGGGTTCAAGCGATTCTCCTGC 
               
               
                   
                 500 
                 CTCAGCCTCCCGAGTAGTTGGGATTACAGGCATGCACCACCACGCCCAGCT 
               
               
                   
                   
                 AATTTTTGTATTTTTAGTAGAGACAAGGTTTCACCGTGATGGCCAGGCTGGT 
               
               
                   
                   
                 CTTGAACTCCAGGACTCAAGTGATGCTCCTGCCTAGGCCTCTCAAAGTGTT 
               
               
                   
                   
                 GGGATTACAGGCGTGAGCCACTGCACCCGGCCTGCACGCGTTCTTTGAAAG 
               
               
                   
                   
                 CAGTCGAGGGGGCGCTAGGTGTGGGCAGGGACGAGCTGGCGCGGCGTCGC 
               
               
                   
                   
                 TGGGTGCACCGCGACCACGGGCAGAGCCACGCGGCGGGAGGACTACAACT 
               
               
                   
                   
                 CCCGGCACACCCCGCGCCGCCCCGCCTCTACTCCCAGAAGGCCGCGGGGGG 
               
               
                   
                   
                 TGGACCGCCTAAGAGGGCGTGCGCTCCCGACATGCCCCGCGGCGCGCCATT 
               
               
                   
                   
                 AACCGCCAGATTTGAATCGCGGGACCCGTTGGCAGAGGTGG 
               
               
                   
               
               
                 257 
                 Survivin 
                 TTGAAAGCAGTCGAGGGGGCGCTAGGTGTGGGCAGGGACGAGCTGGCGCG 
               
               
                   
                 250 
                 GCGTCGCTGGGTGCACCGCGACCACGGGCAGAGCCACGCGGCGGGAGGAC 
               
               
                   
                   
                 TACAACTCCCGGCACACCCCGCGCCGCCCCGCCTCTACTCCCAGAAGGCCG 
               
               
                   
                   
                 CGGGGGGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATGCCCCGCGGC 
               
               
                   
                   
                 GCGCCATTAACCGCCAGATTTGAATCGCGGGACCCGTTGGCAGAGGTGG 
               
               
                   
               
               
                 258 
                 Survivin 
                 TACAACTCCCGGCACACCCCGCGCCGCCCCGCCTCTACTCCCAGAAGGCCG 
               
               
                   
                 150 
                 CGGGGGGTGGACCGCCTAAGAGGGCGTGCGCTCCCGACATGCCCCGCGGC 
               
               
                   
                   
                 GCGCCATTAACCGCCAGATTTGAATCGCGGGACCCGTTGGCAGAGGTGG 
               
               
                   
               
               
                 259 
                 Survivin 
                 CCTAAGAGGGCGTGCGCTCCCGACATGCCCCGCGGCGCGCCATTAACCGCC 
               
               
                   
                 85 
                 AGATTTGAATCGCGGGACCCGTTGGCAGAGGTGG 
               
               
                   
               
               
                 260 
                 ANGPTL-3 
                 AATTCTAGTTTGGTCCTAGATGACCACATATCCATTGTTCCTTCAACGAGCA 
               
               
                   
                 -784 67 
                 CATGGTAAAGAGCCTAGAACACAGAGACACAGAACACAGTGGAGAAAAG 
               
               
                   
                   
                 GGAGTGAAATGTCTTTAATGACACTTACTATATATGGGATTTTGTGACAAT 
               
               
                   
                   
                 ATACAAGGATGGTTAAGACATATAAGGTGATGCAAAAAAACATATTAACA 
               
               
                   
                   
                 ATTATAGTGACAAAAAATGAGGAGCATATAATTATACATTGATTTATACAG 
               
               
                   
                   
                 AGTACCAGAGGAACACAGCATTGAGAGCCGTAACACCACCTGAGGGAGTG 
               
               
                   
                   
                 GAGAAAGGCTTCAGAGAGAAAGTGTTTTTTGGAATGGATCACTGTTTCCAA 
               
               
                   
                   
                 AAGAACTAAAGTACAGTTTGAGAAATGCATACTTAATTCATTACTTTTTTCC 
               
               
                   
                   
                 CCTCAACTTTAATAATAAATTTACCCAACAAAAAAGTTTATTTTTGACTTGT 
               
               
                   
                   
                 AAATCTCTTAAAATCATAAAAAAGTAAAATTAGCTTTTAAAAACAGGTAGT 
               
               
                   
                   
                 CACCATAGCATTGAATGTGTAGTTTATAATACAGCAAAGTTAAATACAATT 
               
               
                   
                   
                 TCAAATTACCTATTAAGTTAGTTGCTCATTTCTTTGATTTCATTTAGCATTGA 
               
               
                   
                   
                 TCTAACTCAATGTGGAAGAAGGTTACATTCGTGCAAGTTAACACGGCTTAA 
               
               
                   
                   
                 TGATTAACTATGTTCACCTACCAACCTTACCTTTTCTGGGCAAATATTGGTA 
               
               
                   
                   
                 TATATAGAGTTAAGAAGTCTAGGTCTGCTTCCAGAAGAAAACAGTTCCACG 
               
               
                   
                   
                 TTGCTTGAAATTGAAAATCAAGATAAAAATGTTCACAATTAAGCTCCTTCTT 
               
               
                   
                   
                 TTTATTGTTCCTCTAGTTATTTCCTCCAGAATTGATCAAGA 
               
               
                   
               
               
                 261 
                 ANGPTL-3 
                 ATAGCATTGAATGTGTAGTTTATAATACAGCAAAGTTAAATACAATTTCAA 
               
               
                   
                 -282 67 
                 ATTACCTATTAAGTTAGTTGCTCATTTCTTTGATTTCATTTAGCATTGATCTA 
               
               
                   
                   
                 ACTCAATGTGGAAGAAGGTTACATTCGTGCAAGTTAACACGGCTTAATGAT 
               
               
                   
                   
                 TAACTATGTTCACCTACCAACCTTACCTTTTCTGGGCAAATATTGGTATATA 
               
               
                   
                   
                 TAGAGTTAAGAAGTCTAGGTCTGCTTCCAGAAGAAAACAGTTCCACGTTGC 
               
               
                   
                   
                 TTGAAATTGAAAATCAAGATAAAAATGTTCACAATTAAGCTCCTTCTTTTTA 
               
               
                   
                   
                 TTGTTCCTCTAGTTATTTCCTCCAGAATTGATCAAGA 
               
               
                   
               
               
                 262 
                 ANGPTL-3 
                 TCAATGTGGAAGAAGGTTACATTCGTGCAAGTTAACACGGCTTAATGATTA 
               
               
                   
                 -175 67 
                 ACTATGTTCACCTACCAACCTTACCTTTTCTGGGCAAATATTGGTATATATA 
               
               
                   
                   
                 GAGTTAAGAAGTCTAGGTCTGCTTCCAGAAGAAAACAGTTCCACGTTGCTT 
               
               
                   
                   
                 GAAATTGAAAATCAAGATAAAAATGTTCACAATTAAGCTCCTTCTTTTTATT 
               
               
                   
                   
                 GTTCCTCTAGTTATTTCCTCCAGAATTGATCAAGA 
               
               
                   
               
               
                 263 
                 AFP 
                 TGGGATGTTTCGAGCAGTCCTGCTGAAGTCCTTTTATATCCTGTTTAAGGGA 
               
               
                   
                 Proximal 
                 TGCCTGTTAACTAGTAACCTTCAGTGAGCAAACATATGACTCTATTTCCTTA 
               
               
                   
                 Compact 
                 CGTTGAAGTTAGGCAATTTGCCAATAATTAACAGAGCAGGGGTCACTTGTA 
               
               
                   
                   
                 TCCTATGTTCAAGGACAAAGACCACTTCAGAGTGGAAAAAAAATCTAAACT 
               
               
                   
                   
                 GTTCAAATAGATTATTTCCCCTGAAGAATAATTCATTCATCTCAACATAAGA 
               
               
                   
                   
                 CATAGATATAGCCATAAAGAAAAGGTAGCAGACTTACTATGTAACTCCAAA 
               
               
                   
                   
                 TACAAGTTCAGGCTATTCATTAGTGGATATATTTCTTGATTATCCAGTTATA 
               
               
                   
                   
                 GTATATTTTATTTTATTTAGTGTATCGCATCTGGTTTAACATA 
               
               
                   
               
               
                 264 
                 AFP 
                 ATGAGGGAAGCGGGTGTGATCCACTTgAaaaCTGCTGGTTCCTTCACCGCAG 
               
               
                   
                 Proximal 
                 GCAGTGCTGGAAGTGGGATGTTTCGAGCAGTCCTGCTGAAGTCCTTTTATA 
               
               
                   
                 Compact 
                 TCCTGTTTAAGGGATGCCTGTTAACTAGTAACCTTCAGTGAGCAAACATAT 
               
               
                   
                 1 st  exon 
                 GACTCTATTTCCTTACGTTGAAGTTAGGCAATTTGCCAATAATTAACAGAGC 
               
               
                   
                   
                 AGGGGTCACTTGTATCCTATGTTCAAGGACAAAGACCACTTCAGAGTGGAA 
               
               
                   
                   
                 AAAAAATCTAAACTGTTCAAATAGATTATTTCCCCTGAAGAATAATTCATT 
               
               
                   
                   
                 CATCTCAACATAAGACATAGATATAGCCATAAAGAAAAGGTAGCAGACTT 
               
               
                   
                   
                 ACTATGTAACTCCAAATACAAGTTCAGGCTATTCATTAGTGGATATATTTCT 
               
               
                   
                   
                 TGATTATCCAGTTATAGTATATTTTATTTTATTTAGTGTATCGCATCTGGTTT 
               
               
                   
                   
                 AACATAG 
               
               
                   
               
               
                 265 
                 AFP Long 
                 ATGAGGGAAGCGGGTGTGATCCACTTgAaaaCTGCTGGTTCCTTCACCGCAG 
               
               
                   
                 TATA 1 st   
                 GCAGTGCTGGAAGTGGGATGTTTCGAGCAGTCCTGCTGAAGTCCTTTTATA 
               
               
                   
                 exon 
                 TCCTGTTTAAGGGATGCCTGTTAACTAGTAACCTTCAGTGAGCAAACATAT 
               
               
                   
                   
                 GACTCTATTTCCTTACGTTGAAGTTAGGCAATTTGCCAATAATTAACAGAGC 
               
               
                   
                   
                 AGGGGTCACTTGTATCCTATGTTCAAGGACAAAGACCACTTCAGAGTGGAA 
               
               
                   
                   
                 AAAAAATCTAAACTGTTCAAATAGATTATTTCCCCTGAAGAATAATTCATT 
               
               
                   
                   
                 CATCTCAACATAAGACATAGATATAGCCATAAAGAAAAGGTAGCAGACTT 
               
               
                   
                   
                 ACTATGTAACTCCAAATACAAGTTCAGGCTATTCATTAGTGGATATATTTCT 
               
               
                   
                   
                 TGATTATCCAGTTATAGTATATTTTATTTTATTTAGTGTATCGCATCTGGTTT 
               
               
                   
                   
                 AACATAGAAAACTTACAGCACAAAACCTGATGAGCCAGCTCCCATTCTAAT 
               
               
                   
                   
                 TTTATGTGCCAAAGAATAATTCCATATGTATGTCACAGGTGCATGGGTCAG 
               
               
                   
                   
                 CTGCAACATCCTCTCAAGCCCTAAGATGATGATGCTAACAGCAACAAATGG 
               
               
                   
                   
                 GCACTGATAGTTTCCATTTCTCTACACATTAGAGTTGATGGAAAACTTTTAA 
               
               
                   
                   
                 AACTTCCCAGTGCGTATCGAAACTAGAACTCAGACGTTGGCGTGTCAGAGT 
               
               
                   
                   
                 CTGTGTGTCTAGAGGTCCAGACATGTTTGCTAAGGCTTCATATGTAGTTGAG 
               
               
                   
                   
                 TTTATTTTTTATTTTTTTAAATTCATGGC 
               
               
                   
               
               
                 266 
                 AFP Long 
                 ATGAGGGAAGCGGGTGTGATCCACTTgAaaaCTGCTGGTTCCTTCACCGCAG 
               
               
                   
                 MRE 
                 GCAGTGCTGGAAGTGGGATGTTTCGAGCAGTCCTGCTGAAGTCCTTTTATA 
               
               
                   
                 TATA 1 st   
                 TCCTGTTTAAGGGATGCCTGTTAACTAGTAACCTTCAGTGAGCAAACATAT 
               
               
                   
                 exon 
                 GACTCTATTTCCTTACGTTGAAGTTAGGCAATTTGCCAATAATTAACAGAGC 
               
               
                   
                   
                 AGGGGTCACTTGTATCCTATGTTCAAGGACAAAGACCACTTCAGAGTGGAA 
               
               
                   
                   
                 AAAAAATCTAAACTGTTCAAATAGATTATTTCCCCTGAAGAATAATTCATT 
               
               
                   
                   
                 CATCTCAACATAAGACATAGATATAGCCATAAAGAAAAGGTAGCAGACTT 
               
               
                   
                   
                 ACTATGTAACTCCAAATACAAGTTCAGGCTATTCATTAGTGGATATATTTCT 
               
               
                   
                   
                 TGATTATCCAGTTATAGTATATTTTATTTTATTTAGTGTATCGCATCTGGTTT 
               
               
                   
                   
                 AACATAGAAAACTTACAGCACAAAACCTGATGAGCCAGCTCCCATTCTAAT 
               
               
                   
                   
                 TTTATGTGCCAAAGAATAATTCCATATGTATGTCACAGGTGCATGGGTCAG 
               
               
                   
                   
                 CTGCAACATCCTCTCAAGCCCTAAGATGATGATGCTAACAGCAACAAATGG 
               
               
                   
                   
                 GCACTGATAGTTTCCATTTCTCTACACATTAGAGTTGATGGAAAACTTTTAA 
               
               
                   
                   
                 AACTTCCCAGTGCGTATCGAAACTAGAACTCAGACGTTGGCGTGTCAGAGT 
               
               
                   
                   
                 CTGTGTGTCTAGAGGTCCAGACATGTTTGCTAAGGCTTCATATGTAGTTGAG 
               
               
                   
                   
                 TTTATTTTTTATTTTTTTAAATTCAGGCGACTGGGTTTGAATTTTGCCCTCTC 
               
               
                   
                   
                 CGTTATCTGCCACATGACTTTGTGTGAGGTtTCTAATACCAACTGCAAACAA 
               
               
                   
                   
                 CCCTAAGCCCACGTGTGCTGTTGCTCAAAGCTTTGTCGCAAATACTGAGCTC 
               
               
                   
                   
                 ACACCACATACCTCTCATAGCTCTATGTCTGGTTCTGTTTGTCACTTCCTGA 
               
               
                   
                   
                 GCCCATGAAACCTCTCAGAAGCAATATGGTTAAACAAACTGGACTTTAGTC 
               
               
                   
                   
                 TATGAAAGGCTCTACCCTTGACTATTCAAACTGTCAGCCAGATGACAAAAA 
               
               
                   
                   
                 CTCAAACCAGCTTTATTCTGGC 
               
               
                   
               
               
                 267 
                 AFP Long 
                 ATGAGGGAAGCGGGTGTGATCCACTTgAaaaCTGCTGGTTCCTTCACCGCAG 
               
               
                   
                 No 
                 GCAGTGCTGGAAGTGGGATGTTTCGAGCAGTCCTGCTGAAGTCCTTTTATA 
               
               
                   
                 Deletions 
                 TCCTGTTTAAGGGATGCCTGTTAACTAGTAACCTTCAGTGAGCAAACATAT 
               
               
                   
                   
                 GACTCTATTTCCTTACGTTGAAGTTAGGCAATTTGCCAATAATTAACAGAGC 
               
               
                   
                   
                 AGGGGTCACTTGTATCCTATGTTCAAGGACAAAGACCACTTCAGAGTGGAA 
               
               
                   
                   
                 AAAAATCTTGCAAATGCTGCAAATGTTCTTCACCATCTAAACTGTTCAAAT 
               
               
                   
                   
                 AGATTATTTCCCCTGAAGAATAATTCATTCATCTCAACATAAGACATAGAT 
               
               
                   
                   
                 ATAGCCATAAAGAAAAGGTAGCAGACTTACTATGTAACTCCAAATACATTC 
               
               
                   
                   
                 TTTTTGAAAGAAATAATAAAATGCACACCATATGCTAGGCACTGAACAAAT 
               
               
                   
                   
                 TGTTTCAGTAGTTCAGGCTATTCATTAGTGGATATATTTCTTGATTATCCAG 
               
               
                   
                   
                 TTATTATTTCGCTCAAAACCATCGGTCAAGTATATTTTATTTTATTTAGTGTA 
               
               
                   
                   
                 TCGCATCTGGTTTAACATAGAAAACTTACAGCACAAAACCTGATGAGCCAG 
               
               
                   
                   
                 CTCCCATTCTAATTTTATGTGCCAAAGAATAATTCCATATGTATGTCACAGG 
               
               
                   
                   
                 TGCATGGGTCAGCTGCAACATCCTCTCAAGCCCTAAGATGATGATGCTAAC 
               
               
                   
                   
                 AGCAACAAATGGGCACTGACATACTTCTGACCCTAAGAGTGCTTCACTCAT 
               
               
                   
                   
                 ACCTTCACCCTCAATGCCGTAGAGTCTATGATAGTTTCCATTTCTCTACACA 
               
               
                   
                   
                 TTAGAGTTGATGGAAAACTTTTAAAACTTCCCAGTGCGTATCGAAACTAGA 
               
               
                   
                   
                 ACTCAGACGTTGGCGTGTCAGAGTCTGTGTGTCTAGAGGTCCAGACATGTT 
               
               
                   
                   
                 TGCTAAGGCTTCATATG 
               
               
                   
               
               
                 268 
                 GPC3 ikb 
                 tAGCCCGACAGAGCAAGAGAGGAGCCGCTACCCAGCCGCCGCAAAAGTTTC 
               
               
                   
                   
                 CTCGCAGCTACCTGGGCGCTGGGCGAGGGCGGGAACAGCTTGGCGGTGCG 
               
               
                   
                   
                 GGGCGGCCCGGGGCGGAGCCTTGTGGGCGTGGCGAGGAGGGACGGGGCGG 
               
               
                   
                   
                 GGCGAGGCAAGGCGAGCCGCGCTGCCTGGAGGACGGCGTGGGGTCGTGTA 
               
               
                   
                   
                 GCTGCTGGCCTGCGGGATGCGGGGCGTGGCAAGGAGCTTAGCTGGGAGAT 
               
               
                   
                   
                 TGGGTTTACCAAGGTGGCGGGCAAGCCTTGGTGGGAGAGGCGCGGGAAGA 
               
               
                   
                   
                 GGATAAGGAGCGTGTGCGGTGGCTCCCGGCAATCCTGCCCTGACACTCGCT 
               
               
                   
                   
                 CGCCGCTGCTCTACACTGGGCGCTCTGGCATAACTACTGCAGAGGGGCTGC 
               
               
                   
                   
                 AGGCTCAGGCACGCTGATTGGCTTCCCAGCAGCAGTCCCCTCTGACTGGCT 
               
               
                   
                   
                 CTGGGAGAAGTTCCCCAGCCTCACTCCTCCTTTCCGCCTCCCTTTGGCCTAC 
               
               
                   
                   
                 AGCCGGGAGGGCTTTTCCTTTTCAGCCTTTGCAAGCTCTCCATCTTCCTTGG 
               
               
                   
                   
                 AGTGGAGTGGAGGTCTGCGGTTTAGGTACCCGACTCGACCCTAGGCCTTCT 
               
               
                   
                   
                 CCCACCCAGATCTGGCTCCTTCTGGCCACCAGAGCCCACACAAGGTTTCCT 
               
               
                   
                   
                 AAGCACAAAATCCCTCTCCTTGCTGTTTTCTGAGAAAGGTTTCTTGGGAACC 
               
               
                   
                   
                 CTTTCCCAATGCAGCTGTGGCCAAGCCCTCAAAGCCTACCCACAAATAGTC 
               
               
                   
                   
                 ACGTTCCAGAGCGCTGGGGACCTCTGGATTTCACAGCCTGGCTCATCTTTGT 
               
               
                   
                   
                 ACCTAAAAGGTCTGGAAGCCCGTGTAGCTTGCTGGGTTTCATTCAATAGAA 
               
               
                   
                   
                 CCACACAAAGTAAATGTGTGCAAATTTAGGCACTTGATCCTGATTCCTAGG 
               
               
                   
                   
                 TGAATCATATCATCTACAGGATAATCACGGGCGACCCTCATAAAGCAAAGT 
               
               
                   
                   
                 GTAGCTGGTGAGAGTAACTCATTCAGGAAATCATTTTACAGATGAAATTCA 
               
               
                   
                   
                 TTAAGTCATGGTTAGTCTGTTTCATACCTGGAGTAGAGCCCTATTTAGAAGA 
               
               
                   
                   
                 TTTCCTGGATGTCAATCCACGTTTCT 
               
               
                   
               
            
           
         
       
     
     In some embodiments, the promoter element comprises a transcription factor response element and a minimal promoter. In some embodiments, the promoter element comprises a mammalian promoter or promoter fragment. In some embodiments, the mammalian promoter or promoter fragment is unique (i.e., the contiguous polynucleic acid includes only one copy of the mammalian promoter or promoter fragment). In other embodiments, the mammalian promoter or promoter fragment is not unique. 
     In some embodiments, a regulatory component comprises a minimal promoter. As used herein, the term “minimal promoter” refers to a nucleic acid sequence that is necessary but not sufficient to initiate expression of an output. In some embodiments, a minimal promoter is naturally occurring. In other embodiments, a minimal promoter is engineered, such as by altering and/or shortening a natural occurring sequence, combining natural occurring sequences, or combining naturally occurring sequences with non-naturally occurring sequences; in each case an engineered minimal promoter is a non-naturally occurring sequence. In some embodiments, the minimal promoter is engineered from a viral or non-viral source. Examples of minimal promoters are known to those having skill in the art. 
     In some embodiments, a regulatory component comprises a transactivator response element, a transcription factor response element, and a minimal promoter. One having skill in the art will appreciate that these elements may be oriented in various configurations. For example, a transactivator response element may be 5′ or 3′ to a promoter element and/or transcription factor response element; a transcription factor response element may be 5′ or 3′ to a promoter element and/or transactivator response element; a promoter element may be 5′ or 3′ to a transcription factor response element and/or a transactivator response element. 
     In some embodiments, the regulatory component of a cassette comprises, from 5′ to 3′: a transactivator response element, a transcription factor response element, and a minimal promoter. In some embodiments, a regulatory component comprises from 5′ to 3′: a transcription factor response element, a transactivator response element, and a minimal promoter. 
     In some embodiments, the regulatory component of a cassette comprises a transactivator response element and a promoter element. In some embodiments, the regulatory component of a cassette comprises, from 5′ to 3′: a transactivator response element and a promoter element. In some embodiments, the regulatory component of a cassette comprises a transactivator response element, a promoter element and a minimal promoter. In some embodiments, the regulatory component of a cassette comprises, from 5′ to 3′: a transactivator response element, a promoter element and a minimal promoter. In some embodiments, the regulatory component of a cassette comprises, from 5′ to 3′: a promoter element and a transactivator response element. In some embodiments, the regulatory component of a cassette comprises, from 5′ to 3′: a promoter element, a transactivator response element and a minimal promoter. In some embodiments, the promoter element is a mammalian promoter. In some embodiments, the promoter element is a promoter fragment. 
     (v) Exemplary Contiguous Polynucleic Acids 
     In some embodiments, a contiguous polynucleic acid molecule comprises a gene circuit having a single cassette. For example, in some embodiments, a contiguous polynucleic acid molecule comprises a cassette encoding an RNA whose expression is operably linked to a transactivator response element, wherein the RNA comprises: (i) a nucleic acid sequence of an output; (ii) a nucleic acid sequence of a transactivator; and (iii) a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof); wherein the transactivator, when expressed as a protein, binds and transactivates the transactivator response element. 
     In some embodiments, the mRNA further comprises a nucleic acid sequence of a polycistronic expression element. The term “polycistronic response element,” as used herein, refers to a nucleic acid sequence that facilitates the generation of two or more proteins from a single mRNA. A polycistronic response element may comprise a polynucleic acid encoding an internal recognition sequence (IRES) or a 2A peptide. See e.g., Liu et al., Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Sci. Rep. 2017 May 19; 7(1): 2193. In some embodiments, the polycistronic expression element separates the nucleic acid sequences of the output and the transactivator. 
     In some embodiments, the mRNA comprises a 3′ UTR, wherein the 3′ UTR comprises a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). In some embodiments, the mRNA comprises a 5′ UTR, wherein the 5′ UTR comprises a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). 
     In some embodiments, the contiguous polynucleic acid molecules comprise, from 5′ to 3′: (i) an upstream regulatory component comprising the transactivator response element and the transcription factor response element; (ii) the nucleic acid sequence encoding the output and the transactivator; and (iii) a downstream component comprising a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). 
     In some embodiments, the contiguous polynucleic acid molecules comprise, from 5′ to 3′: (i) an upstream regulatory component comprising the transcription factor response element and the transactivator response element; (ii) the nucleic acid sequence encoding the output and the transactivator; and (iii) a downstream component comprising a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). 
     In some embodiments, the contiguous polynucleic acid molecules comprise, from 5′ to 3′: (i) an upstream regulatory component comprising the transactivator response element and the transcription factor response element; (ii) the nucleic acid sequence encoding the transactivator and the output; and (iii) a downstream component comprising a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). 
     In some embodiments, the contiguous polynucleic acid molecules comprise, from 5′ to 3′: (i) an upstream regulatory component comprising the transcription factor response element and the transactivator response element; (ii) the nucleic acid sequence encoding the transactivator and the output; and (iii) a downstream component comprising a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). 
     In some embodiments, the contiguous polynucleic acid molecules comprise, from 5′ to 3′: (i) an upstream regulatory component comprising a promoter element and the transactivator response element; (ii) the nucleic acid sequence encoding the transactivator and the output; and (iii) a downstream component comprising a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). 
     In some embodiments, the contiguous polynucleic acid molecules comprise, from 5′ to 3′: (i) an upstream regulatory component comprising the transactivator response element and a promoter element; (ii) the nucleic acid sequence encoding the transactivator and the output; and (iii) a downstream component comprising a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). 
     In some embodiments, the promoter element comprises a mammalian promoter or promoter fragment. 
     In some embodiments, a contiguous polynucleic acid molecule comprises a gene circuit having multiple cassettes. For example, in some embodiments, a contiguous polynucleic acid molecule comprising: a) a first cassette encoding a first RNA whose expression is operably linked to a transactivator response element, wherein the first RNA comprises: (i) a nucleic acid sequence of an output; and (ii) a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof); and b) a second cassette encoding a second RNA, wherein the second RNA comprises a nucleic acid sequence of a transactivator; wherein the transactivator of the second cassette, when expressed as a protein, binds and transactivates the transactivator response element of the first cassette. 
     In some embodiments, the first RNA comprises a 3′ UTR, and the 3′ UTR comprises a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). In some embodiments, the first RNA comprises a 5′ UTR, and the 5′ UTR comprises a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). 
     In some embodiments, the second RNA comprises a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). In some embodiments, the second RNA comprises a 3′ UTR, and the 3′ UTR comprises a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). In some embodiments, the second RNA comprises a 5′ UTR, and the 5′ UTR comprises a miRNA target site (e.g., a let-7c target site, a miR-22 target site, a miR-26b target site, or a combination thereof). In some embodiments, at least one miRNA target site of the first cassette and at least one miRNA target site of the second cassette are the same nucleic acid sequence or are different sequences regulated by the same miRNA. 
     In some embodiments, the first RNA is operably linked to a transcription factor response element. In some embodiments, the second RNA is operably linked to a transcription factor response element. In some embodiments, the transcription factor response element of the first cassette and the transcription factor response element of the second cassette consist of identical nucleic acid sequences. In some embodiments, the transcription factor response element of the first cassette and the transcription factor response element of the second cassette consist of different nucleic acid sequences. In some embodiments, either the first cassette or the second cassette or both, comprise at least two, at least three . . . types of transcription factor response elements. 
     In some embodiments, the first cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising the transactivator response element and the transcription factor response element; (ii) the nucleic acid sequence encoding the output; and (iii) a downstream component comprising a let-7c target site; and the second cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising the transcription factor response element; (ii) the nucleic acid sequence encoding the transactivator; and (iii) a downstream component comprising a let-7c target site. 
     In some embodiments, the first cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising the transcription factor response element and the transactivator response element; (ii) the nucleic acid sequence encoding the output; and (iii) a downstream component comprising a let-7c target site; and the second cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising the transcription factor response element; (ii) the nucleic acid sequence encoding the transactivator; and (iii) a downstream component comprising a let-7c target site. 
     In some embodiments, the first cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising the transactivator response element and the transcription factor response element; (ii) the nucleic acid sequence encoding the output; and (iii) a downstream component comprising a let-7c target site; and the second cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising a promoter element; (ii) the nucleic acid sequence encoding the transactivator; and (iii) a downstream component comprising a let-7c target site. 
     In some embodiments, the first cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising the transcription factor response element and the transactivator response element; (ii) the nucleic acid sequence encoding the output; and (iii) a downstream component comprising a let-7c target site; and the second cassette comprises, from 5′ to 3′: (i) an upstream regulatory component comprising promoter element; (ii) the nucleic acid sequence encoding the transactivator; and (iii) a downstream component comprising a let-7c target site. 
     In some embodiments, the upstream regulatory component of the first cassette comprises a promoter element in addition to the transcription factor response element. In some embodiments, a promoter element replaces the transcription factor response element. In some embodiments, the promoter element comprises a mammalian promoter or promoter fragment. 
     In some embodiments, the first cassette and the second cassette are in a convergent orientation. In some embodiments, the first cassette and the second cassette are in a divergent orientation. In some embodiments, the first cassette and the second cassette are in a head-to-tail orientation. 
     The first and/or second cassette may be flanked by one or more insulators (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 insulators). For example, in some embodiments, the first cassette or the second cassette is flanked by an insulator. In some embodiments, both the first cassette and the second cassette are flanked by an insulator. In some embodiments, the first cassette or the second cassette is flanked on both sides by an insulator. 
     Exemplary contiguous polynucleic acids are listed in TABLE 6. In some embodiments, a contiguous polynucleic acid comprises a nucleic acid sequence listed in TABLE 6 or a nucleic acid sequence having at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% identity to a nucleic acid sequence listed in TABLE 6. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Exemplary contiguous polynucleic acids. 
               
            
           
           
               
               
               
            
               
                 Seq ID 
                 Name 
                 SEQUENCE 
               
               
                   
               
               
                 269 
                 ITR.C.CMV. 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTC 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCAAGCACTCTGATTTGACAATTAAAGCACTCTGATTTGACAATTAAAGCACT 
               
               
                   
                   
                 CTGATTTGACAATTAAAGCACTCTGATTTGACAATTAGTCGACCTCGAGAGATCTACGGGTG 
               
               
                   
                   
                 GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCA 
               
               
                   
                   
                 CCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATA 
               
               
                   
                   
                 TTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGG 
               
               
                   
                   
                 CCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATC 
               
               
                   
                   
                 TCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGC 
               
               
                   
                   
                 ATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGC 
               
               
                   
                   
                 CAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGG 
               
               
                   
                   
                 GATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCG 
               
               
                   
                   
                 GACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTGGCCACTCCCTCTCTGCGCGCTCGCT 
               
               
                   
                   
                 CGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTC 
               
               
                   
                   
                 AGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 270 
                 ITR.c.Let7c. 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 Mcherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTC 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTGATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCAACCATACAACCTACTACCTCAAACCATACAACCTACTACCTCAAACCATA 
               
               
                   
                   
                 CAACCTACTACCTCAAACCATACAACCTACTACCTCAGTCGACCTCGAGAGATCTACGGGTG 
               
               
                   
                   
                 GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCA 
               
               
                   
                   
                 CCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATA 
               
               
                   
                   
                 TTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGG 
               
               
                   
                   
                 CCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATC 
               
               
                   
                   
                 TCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGC 
               
               
                   
                   
                 ATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGC 
               
               
                   
                   
                 CAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGG 
               
               
                   
                   
                 GATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCG 
               
               
                   
                   
                 GACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTGGCCACTCCCTCTCTGCGCGCTCGCT 
               
               
                   
                   
                 CGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTC 
               
               
                   
                   
                 AGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 271 
                 ITR.C.TF- 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 AND.Mcherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCCTAGGCTTCGAATCGATGAATTCGA 
               
               
                   
                   
                 AGCTTCTACCCACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTCAAACTCGAA 
               
               
                   
                   
                 GTCGGCCATATCCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCCGGACCCGGG 
               
               
                   
                   
                 GAATCCCCGTCCCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCATGCGCCATCGC 
               
               
                   
                   
                 CACGTCCTCGCCGTCTAAGTGGAGCTCGTCCCCCAGGCTGACATCGGTCGGGGGGGCCGTCG 
               
               
                   
                   
                 ACAGTCTGCGCGTGTGTCCCGCGGGGAGAAAGGACAGGCGCGGAGCCGCCAGCCCCGCCTC 
               
               
                   
                   
                 TTCGGGGGCGTCGTCGTCCGGGAGATCGAGCAGGCCCTCGATGGTAGACCCGTAATTGTTTT 
               
               
                   
                   
                 TCGTACGCGCGCGGCTGTACGCGGAGGCCTGTTCGACCATCGCGTCGATGCCCGCGACGAG 
               
               
                   
                   
                 CAGGTCGAGGGCGAACTCGAAGTCCCGGTCCAGCATCTCCGCCACGGTGTCGCCGCCCCGG 
               
               
                   
                   
                 GCCGCCATGATGTCCTGCGCGTCCTCGATGACGCCCGCGGTGTCCGGCACCTCGGTCACCGC 
               
               
                   
                   
                 GGTCATCGAGTCCTGGAAGTACTCCTCCGGACTCAGCCCGGTGTCCGCCACCCGGGCGAGG 
               
               
                   
                   
                 AAGCGGCCCTCGATGGTGCCGTAGCCGTAGACGAACTGGAAGACGGCCGAGATGGCGCCGG 
               
               
                   
                   
                 TCAGGCGGTGCGCGGGCAGCCCGCTGCGGCGCACGACGTTCTGCACCGCGCGGGAGAAGGC 
               
               
                   
                   
                 CAGCGAGTGCGGGCCGATGTTGAGGTAGGTGCCGACCAGCCGGGACGACCAGGGGTGGCG 
               
               
                   
                   
                 CACCAGCAGCGCCCGGTTCTCCCGGGCCAGGGCCCGCAGTTCCTCGCGCCAGTCGAGCCCG 
               
               
                   
                   
                 GCGTCCGGGTCCGGGTGGCGCAGCTCGCCGAAGACGGCGTCCAGGGCGAGCTCGAGCAACT 
               
               
                   
                   
                 GGTCCTTGGTGTCGACGTACCAGTACACGGACATCGCGGTGACGTTCAGCTCGGCGGCCAG 
               
               
                   
                   
                 GCGGCGCATCGAGAACCCCGTCAGGCCCTCCGTGTCCAGCAGCCGGACGGTGACCCCGGTG 
               
               
                   
                   
                 ATCCGGTCCCGGTCGAGCCCGGACGGCTGCCCCCCACGGCGACCGCCGCGCCGCCCCTCCCC 
               
               
                   
                   
                 CGACAGCCACACGCTGTCCCGCGGCCCCTCCCGCCCTGCCTTCGCCATGCGCACCTCTCCTC 
               
               
                   
                   
                 GACTCATACCGGTAGCGCTAGCGATGAGCTCTGGTAGTAGACTAGTGGCCCCCATTATATAC 
               
               
                   
                   
                 CCTCTAGAGCATATGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTA 
               
               
                   
                   
                 GTCTCACAAAGAGGGCTTTGTGTAGGGCGCGCCCCCGTAGCTTGGCGTAATCACATGTCCGT 
               
               
                   
                   
                 CGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTGCAAGGCGATTAAGTTGGGTAACGC 
               
               
                   
                   
                 CAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGACATGTGAAATAGCGCTGTACAGCG 
               
               
                   
                   
                 TATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGGCGCGCCAGTTAA 
               
               
                   
                   
                 TAATTAACTAGTTAATAATTAACTAGTTAATAATTAACTCATATGCTCTAGAGGGTATATAA 
               
               
                   
                   
                 TGGGGGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCGCCACCATGGTGAGC 
               
               
                   
                   
                 AAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG 
               
               
                   
                   
                 AGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACG 
               
               
                   
                   
                 AGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGA 
               
               
                   
                   
                 CATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCC 
               
               
                   
                   
                 CCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAG 
               
               
                   
                   
                 GACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTCCAGGACGGCGAGTTCATCTACA 
               
               
                   
                   
                 AGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCAT 
               
               
                   
                   
                 GGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATC 
               
               
                   
                   
                 AAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTAC 
               
               
                   
                   
                 AAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCA 
               
               
                   
                   
                 CCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTG 
               
               
                   
                   
                 CACCGGCGGCATGGACGAGCTGTACAAGTAGGGTACCGTCGACCTCGAGAGATCTACGGGT 
               
               
                   
                   
                 GGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCC 
               
               
                   
                   
                 ACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAAT 
               
               
                   
                   
                 ATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGG 
               
               
                   
                   
                 GCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAA 
               
               
                   
                   
                 TCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGG 
               
               
                   
                   
                 CATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGG 
               
               
                   
                   
                 CCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTG 
               
               
                   
                   
                 GGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGC 
               
               
                   
                   
                 GGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCG 
               
               
                   
                   
                 CTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCC 
               
               
                   
                   
                 TCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 272 
                 ITR.HCC.V2. 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCCTAGGTGAGGTAGTAGGTTGTATGG 
               
               
                   
                   
                 TTTGAGGTAGTAGGTTGTATGGTTTGAGGTAGTAGGTTGTATGGTTTGAGGTAGTAGGTTGT 
               
               
                   
                   
                 ATGGTTATCGATGAATTCGAAGCTTCTACCCACCGTACTCGTCAATTCCAAGGGCATCGGTA 
               
               
                   
                   
                 AACATCTGCTCAAACTCGAAGTCGGCCATATCCAGAGCGCCGTAGGGGGCGGAGTCGTGGG 
               
               
                   
                   
                 GGGTAAATCCCGGACCCGGGGAATCCCCGTCCCCCAACATGTCCAGATCGAAATCGTCTAG 
               
               
                   
                   
                 CGCGTCGGCATGCGCCATCGCCACGTCCTCGCCGTCTAAGTGGAGCTCGTCCCCCAGGCTGA 
               
               
                   
                   
                 CATCGGTCGGGGGGGCCGTCGACAGTCTGCGCGTGTGTCCCGCGGGGAGAAAGGACAGGCG 
               
               
                   
                   
                 CGGAGCCGCCAGCCCCGCCTCTTCGGGGGCGTCGTCGTCCGGGAGATCGAGCAGGCCCTCG 
               
               
                   
                   
                 ATGGTAGACCCGTAATTGTTTTTCGTACGCGCGCGGCTGTACGCGGAGGCCTGTTCGACCAT 
               
               
                   
                   
                 CGCGTCGATGCCCGCGACGAGCAGGTCGAGGGCGAACTCGAAGTCCCGGTCCAGCATCTCC 
               
               
                   
                   
                 GCCACGGTGTCGCCGCCCCGGGCCGCCATGATGTCCTGCGCGTCCTCGATGACGCCCGCGGT 
               
               
                   
                   
                 GTCCGGCACCTCGGTCACCGCGGTCATCGAGTCCTGGAAGTACTCCTCCGGACTCAGCCCGG 
               
               
                   
                   
                 TGTCCGCCACCCGGGCGAGGAAGCGGCCCTCGATGGTGCCGTAGCCGTAGACGAACTGGAA 
               
               
                   
                   
                 GACGGCCGAGATGGCGCCGGTCAGGCGGTGCGCGGGCAGCCCGCTGCGGCGCACGACGTTC 
               
               
                   
                   
                 TGCACCGCGCGGGAGAAGGCCAGCGAGTGCGGGCCGATGTTGAGGTAGGTGCCGACCAGCC 
               
               
                   
                   
                 GGGACGACCAGGGGTGGCGCACCAGCAGCGCCCGGTTCTCCCGGGCCAGGGCCCGCAGTTC 
               
               
                   
                   
                 CTCGCGCCAGTCGAGCCCGGCGTCCGGGTCCGGGTGGCGCAGCTCGCCGAAGACGGCGTCC 
               
               
                   
                   
                 AGGGCGAGCTCGAGCAACTGGTCCTTGGTGTCGACGTACCAGTACACGGACATCGCGGTGA 
               
               
                   
                   
                 CGTTCAGCTCGGCGGCCAGGCGGCGCATCGAGAACCCCGTCAGGCCCTCCGTGTCCAGCAG 
               
               
                   
                   
                 CCGGACGGTGACCCCGGTGATCCGGTCCCGGTCGAGCCCGGACGGCTGCCCCCCACGGCGA 
               
               
                   
                   
                 CCGCCGCGCCGCCCCTCCCCCGACAGCCACACGCTGTCCCGCGGCCCCTCCCGCCCTGCCTT 
               
               
                   
                   
                 CGCCATGCGCACCTCTCCTCGACTCATACCGGTAGCGCTAGCGATGAGCTCTGGTAGTAGAC 
               
               
                   
                   
                 TAGTGGCCCCCATTATATACCCTCTAGAGCATATGTCTCACAAAGAGGGCTTTGTGTAGTCT 
               
               
                   
                   
                 CACAAAGAGGGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTAGGGCGCGCCCCCGTAGC 
               
               
                   
                   
                 TTGGCGTAATCACATGTCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTGCAAG 
               
               
                   
                   
                 GCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGACATG 
               
               
                   
                   
                 TGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACA 
               
               
                   
                   
                 CCGTACGGCGCGCCAGTTAATAATTAACTAGTTAATAATTAACTAGTTAATAATTAACTCAT 
               
               
                   
                   
                 ATGCTCTAGAGGGTATATAATGGGGGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTG 
               
               
                   
                   
                 GATCCGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCAT 
               
               
                   
                   
                 GCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAG 
               
               
                   
                   
                 GGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGC 
               
               
                   
                   
                 CCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGT 
               
               
                   
                   
                 GAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGG 
               
               
                   
                   
                 AGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTCCA 
               
               
                   
                   
                 GGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCC 
               
               
                   
                   
                 GTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACG 
               
               
                   
                   
                 GCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGACG 
               
               
                   
                   
                 CTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGT 
               
               
                   
                   
                 CAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAA 
               
               
                   
                   
                 CGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGGGTACCAACC 
               
               
                   
                   
                 ATACAACCTACTACCTCAAACCATACAACCTACTACCTCAAACCATACAACCTACTACCTCA 
               
               
                   
                   
                 AACCATACAACCTACTACCTCAAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCC 
               
               
                   
                   
                 TCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTT 
               
               
                   
                   
                 GCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGG 
               
               
                   
                   
                 AGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCT 
               
               
                   
                   
                 GGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTC 
               
               
                   
                   
                 CTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTT 
               
               
                   
                   
                 GTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCA 
               
               
                   
                   
                 GGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCT 
               
               
                   
                   
                 TCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCGGACCGAGCGGCCGCAGGAACCCCTAG 
               
               
                   
                   
                 TGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAG 
               
               
                   
                   
                 GTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCC 
               
               
                   
                   
                 TGCAGG 
               
               
                   
               
               
                 273 
                 ITR.C.HNF1- 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 FB.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCCTAGGGGGTCCACTTGCTCCTGGGC 
               
               
                   
                   
                 CCACACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGT 
               
               
                   
                   
                 GAAAGGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGA 
               
               
                   
                   
                 GGTGGTTGCCATGGGGACCTGGATGCTGTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGG 
               
               
                   
                   
                 AAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGC 
               
               
                   
                   
                 AAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGAA 
               
               
                   
                   
                 TTCGAAGCTTACGACGGACATGTGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGG 
               
               
                   
                   
                 TGTACGAGTATCTTCCCGTACACCGTACGGCGCGCCAGTTAATAATTAACTAGTTAATAATT 
               
               
                   
                   
                 AACTAGTTAATAATTAACTCATATGCTCTAGAGGGTATATAATGGGGGCCACTAGTCTACTA 
               
               
                   
                   
                 CCAGAGCTCATCGCTAGCGCTGGATCCGCCACCATGGTGAGCAAGGGCGAGGAGGATAACA 
               
               
                   
                   
                 TGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCA 
               
               
                   
                   
                 CGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAA 
               
               
                   
                   
                 GCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCA 
               
               
                   
                   
                 TGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCC 
               
               
                   
                   
                 TTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCG 
               
               
                   
                   
                 TGACCCAGGACTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCAC 
               
               
                   
                   
                 CAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCC 
               
               
                   
                   
                 GAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTG 
               
               
                   
                   
                 AAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTG 
               
               
                   
                   
                 CAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACT 
               
               
                   
                   
                 ACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGA 
               
               
                   
                   
                 GCTGTACAAGTCCGGAAGAGCCGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGA 
               
               
                   
                   
                 GGAAAATCCCGGGCCCAGATCTATGAGTCGAGGAGAGGTGCGCATGGCGAAGGCAGGGCG 
               
               
                   
                   
                 GGAGGGGCCGCGGGACAGCGTGTGGCTGTCGGGGGAGGGGCGGCGCGGCGGTCGCCGTGG 
               
               
                   
                   
                 GGGGCAGCCGTCCGGGCTCGACCGGGACCGGATCACCGGGGTCACCGTCCGGCTGCTGGAC 
               
               
                   
                   
                 ACGGAGGGCCTGACGGGGTTCTCGATGCGCCGCCTGGCCGCCGAGCTGAACGTCACCGCGA 
               
               
                   
                   
                 TGTCCGTGTACTGGTACGTCGACACCAAGGACCAGTTGCTCGAGCTCGCCCTGGACGCCGTC 
               
               
                   
                   
                 TTCGGCGAGCTGCGCCACCCGGACCCGGACGCCGGGCTCGACTGGCGCGAGGAACTGCGGG 
               
               
                   
                   
                 CCCTGGCCCGGGAGAACCGGGCGCTGCTGGTGCGCCACCCCTGGTCGTCCCGGCTGGTCGG 
               
               
                   
                   
                 CACCTACCTCAACATCGGCCCGCACTCGCTGGCCTTCTCCCGCGCGGTGCAGAACGTCGTGC 
               
               
                   
                   
                 GCCGCAGCGGGCTGCCCGCGCACCGCCTGACCGGCGCCATCTCGGCCGTCTTCCAGTTCGTC 
               
               
                   
                   
                 TACGGCTACGGCACCATCGAGGGCCGCTTCCTCGCCCGGGTGGCGGACACCGGGCTGAGTC 
               
               
                   
                   
                 CGGAGGAGTACTTCCAGGACTCGATGACCGCGGTGACCGAGGTGCCGGACACCGCGGGCGT 
               
               
                   
                   
                 CATCGAGGACGCGCAGGACATCATGGCGGCCCGGGGCGGCGACACCGTGGCGGAGATGCT 
               
               
                   
                   
                 GGACCGGGACTTCGAGTTCGCCCTCGACCTGCTCGTCGCGGGCATCGACGCGATGGTCGAA 
               
               
                   
                   
                 CAGGCCTCCGCGTACAGCCGCGCGCATGATGAGTTTCCCACCATGGTGTTTCCTTCTGGGCA 
               
               
                   
                   
                 GATCAGCCAGGCCTCGGCCTTGGCCCCGGCCCCTCCCCAAGTCCTGCCCCAGGCTCCAGCCC 
               
               
                   
                   
                 CTGCCCCTGCTCCAGCCATGGTATCAGCTCTGGCCCAGGCCCCAGCCCCTGTCCCAGTCCTA 
               
               
                   
                   
                 GCCCCAGGCCCTCCTCAGGCTGTGGCCCCACCTGCCCCCAAGCCCACCCAGGCTGGGGAAG 
               
               
                   
                   
                 GAACGCTGTCAGAGGCCCTGCTGCAGCTGCAGTTTGATGATGAAGACCTGGGGGCCTTGCTT 
               
               
                   
                   
                 GGCAACAGCACAGACCCAGCTGTGTTCACAGACCTGGCATCCGTCGACAACTCCGAGTTTG 
               
               
                   
                   
                 AGCAGCTGCTGAACCAGGGCATACCTGTGGCCCCCCACACAACTGAGCCCATGCTGATGGA 
               
               
                   
                   
                 GTACCCTGAGGCTATAACTCGCCTAGTGACAGGGGCCCAGAGGCCCCCCGACCCAGCTCCT 
               
               
                   
                   
                 GCTCCACTGGGGGCCCCGGGGCTCCCCAATGGCCTCCTTTCAGGAGATGAAGACTTCTCCTC 
               
               
                   
                   
                 CATTGCGGACATGGACTTCTCAGCCCTGCTGAGTCAGATCAGCTCCTAAGGAAGCTTGGTAC 
               
               
                   
                   
                 CGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGC 
               
               
                   
                   
                 CCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTT 
               
               
                   
                   
                 TGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGG 
               
               
                   
                   
                 GCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCA 
               
               
                   
                   
                 GTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCA 
               
               
                   
                   
                 GCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTG 
               
               
                   
                   
                 GTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCT 
               
               
                   
                   
                 ACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTC 
               
               
                   
                   
                 CTTCTGATTTTGTAGGTAACCACGTGCGGACCGAGCGGCCGCAGGAACCCCTAGTGATGGA 
               
               
                   
                   
                 GTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCC 
               
               
                   
                   
                 GACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 274 
                 ITR.C.SOX- 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 FB.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCCTAGGGGGTCCACTTGCTCCTGGGC 
               
               
                   
                   
                 CCACACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGT 
               
               
                   
                   
                 GAAAGGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGA 
               
               
                   
                   
                 GGTGGTTGCCATGGGGACCTGGATGCTGTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGG 
               
               
                   
                   
                 AAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGC 
               
               
                   
                   
                 AAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGAA 
               
               
                   
                   
                 TTCGAAGCTTACGACGGACATGTGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGG 
               
               
                   
                   
                 TGTACGAGTATCTTCCCGTACACCGTACGGCGCGCCCTACACAAAGCCCTCTTTGTGAGACT 
               
               
                   
                   
                 ACACAAAGCCCTCTTTGTGAGACTACACAAAGCCCTCTTTGTGAGACATATGCTCTAGAGGG 
               
               
                   
                   
                 TATATAATGGGGGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCGCCACCAT 
               
               
                   
                   
                 GGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTG 
               
               
                   
                   
                 CACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGC 
               
               
                   
                   
                 CCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCG 
               
               
                   
                   
                 CCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCC 
               
               
                   
                   
                 GACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAA 
               
               
                   
                   
                 CTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTCCAGGACGGCGAGTTC 
               
               
                   
                   
                 ATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGA 
               
               
                   
                   
                 AGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGG 
               
               
                   
                   
                 CGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGAC 
               
               
                   
                   
                 CACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTG 
               
               
                   
                   
                 GACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCC 
               
               
                   
                   
                 GCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTCCGGAAGAGCCGAGGGCAGGGGAA 
               
               
                   
                   
                 GTCTTCTAACATGCGGGGACGTGGAGGAAAATCCCGGGCCCAGATCTATGAGTCGAGGAGA 
               
               
                   
                   
                 GGTGCGCATGGCGAAGGCAGGGCGGGAGGGGCCGCGGGACAGCGTGTGGCTGTCGGGGGA 
               
               
                   
                   
                 GGGGCGGCGCGGCGGTCGCCGTGGGGGGCAGCCGTCCGGGCTCGACCGGGACCGGATCACC 
               
               
                   
                   
                 GGGGTCACCGTCCGGCTGCTGGACACGGAGGGCCTGACGGGGTTCTCGATGCGCCGCCTGG 
               
               
                   
                   
                 CCGCCGAGCTGAACGTCACCGCGATGTCCGTGTACTGGTACGTCGACACCAAGGACCAGTT 
               
               
                   
                   
                 GCTCGAGCTCGCCCTGGACGCCGTCTTCGGCGAGCTGCGCCACCCGGACCCGGACGCCGGG 
               
               
                   
                   
                 CTCGACTGGCGCGAGGAACTGCGGGCCCTGGCCCGGGAGAACCGGGCGCTGCTGGTGCGCC 
               
               
                   
                   
                 ACCCCTGGTCGTCCCGGCTGGTCGGCACCTACCTCAACATCGGCCCGCACTCGCTGGCCTTC 
               
               
                   
                   
                 TCCCGCGCGGTGCAGAACGTCGTGCGCCGCAGCGGGCTGCCCGCGCACCGCCTGACCGGCG 
               
               
                   
                   
                 CCATCTCGGCCGTCTTCCAGTTCGTCTACGGCTACGGCACCATCGAGGGCCGCTTCCTCGCC 
               
               
                   
                   
                 CGGGTGGCGGACACCGGGCTGAGTCCGGAGGAGTACTTCCAGGACTCGATGACCGCGGTGA 
               
               
                   
                   
                 CCGAGGTGCCGGACACCGCGGGCGTCATCGAGGACGCGCAGGACATCATGGCGGCCCGGG 
               
               
                   
                   
                 GCGGCGACACCGTGGCGGAGATGCTGGACCGGGACTTCGAGTTCGCCCTCGACCTGCTCGT 
               
               
                   
                   
                 CGCGGGCATCGACGCGATGGTCGAACAGGCCTCCGCGTACAGCCGCGCGCATGATGAGTTT 
               
               
                   
                   
                 CCCACCATGGTGTTTCCTTCTGGGCAGATCAGCCAGGCCTCGGCCTTGGCCCCGGCCCCTCC 
               
               
                   
                   
                 CCAAGTCCTGCCCCAGGCTCCAGCCCCTGCCCCTGCTCCAGCCATGGTATCAGCTCTGGCCC 
               
               
                   
                   
                 AGGCCCCAGCCCCTGTCCCAGTCCTAGCCCCAGGCCCTCCTCAGGCTGTGGCCCCACCTGCC 
               
               
                   
                   
                 CCCAAGCCCACCCAGGCTGGGGAAGGAACGCTGTCAGAGGCCCTGCTGCAGCTGCAGTTTG 
               
               
                   
                   
                 ATGATGAAGACCTGGGGGCCTTGCTTGGCAACAGCACAGACCCAGCTGTGTTCACAGACCT 
               
               
                   
                   
                 GGCATCCGTCGACAACTCCGAGTTTCAGCAGCTGCTGAACCAGGGCATACCTGTGGCCCCCC 
               
               
                   
                   
                 ACACAACTGAGCCCATGCTGATGGAGTACCCTGAGGCTATAACTCGCCTAGTGACAGGGGC 
               
               
                   
                   
                 CCAGAGGCCCCCCGACCCAGCTCCTGCTCCACTGGGGGCCCCGGGGCTCCCCAATGGCCTCC 
               
               
                   
                   
                 TTTCAGGAGATGAAGACTTCTCCTCCATTGCGGACATGGACTTCTCAGCCCTGCTGAGTCAG 
               
               
                   
                   
                 ATCAGCTCCTAAGGAAGCTTGGTACCGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGTG 
               
               
                   
                   
                 ACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTC 
               
               
                   
                   
                 CTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGG 
               
               
                   
                   
                 AGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTC 
               
               
                   
                   
                 TATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTG 
               
               
                   
                   
                 GGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCA 
               
               
                   
                   
                 GGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCT 
               
               
                   
                   
                 CCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCG 
               
               
                   
                   
                 TGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCGGACCGAGCGG 
               
               
                   
                   
                 CCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGA 
               
               
                   
                   
                 GGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAG 
               
               
                   
                   
                 CGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 275 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 26B.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTG 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCACCTATCCTGAATTACTTGAAACCTATCCTGAATTACTTGAAACCTATCCT 
               
               
                   
                   
                 GAATTACTTGAAACCTATCCTGAATTACTTGAAGTCGACCTCGAGAGATCTACGGGTGGCAT 
               
               
                   
                   
                 CCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAG 
               
               
                   
                   
                 CCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTAT 
               
               
                   
                   
                 GGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTG 
               
               
                   
                   
                 CGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCG 
               
               
                   
                   
                 CCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGC 
               
               
                   
                   
                 ATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAG 
               
               
                   
                   
                 GCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATT 
               
               
                   
                   
                 ACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCGGAC 
               
               
                   
                   
                 CGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGC 
               
               
                   
                   
                 TCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGT 
               
               
                   
                   
                 GAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 276 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 22.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTG 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCACAGTTCTTCAACTGGCAGCTTACAGTTCTTCAACTGGCAGCTTACAGTTC 
               
               
                   
                   
                 TTCAACTGGCAGCTTACAGTTCTTCAACTGGCAGCTTGTCGACCTCGAGAGATCTACGGGTG 
               
               
                   
                   
                 GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCA 
               
               
                   
                   
                 CCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATA 
               
               
                   
                   
                 TTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGG 
               
               
                   
                   
                 CCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATC 
               
               
                   
                   
                 TCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGC 
               
               
                   
                   
                 ATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGC 
               
               
                   
                   
                 CAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGG 
               
               
                   
                   
                 GATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCG 
               
               
                   
                   
                 GACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTGGCCACTCCCTCTCTGCGCGCTCGCT 
               
               
                   
                   
                 CGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTC 
               
               
                   
                   
                 AGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 277 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 126.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTG 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCCGTGTTCACAGCGGACCTTGATCGTGTTCACAGCGGACCTTGATCGTGTTC 
               
               
                   
                   
                 ACAGCGGACCTTGATCGTGTTCACAGCGGACCTTGATGTCGACCTCGAGAGATCTACGGGTG 
               
               
                   
                   
                 GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCA 
               
               
                   
                   
                 CCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATA 
               
               
                   
                   
                 TTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGG 
               
               
                   
                   
                 CCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATC 
               
               
                   
                   
                 TCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGC 
               
               
                   
                   
                 ATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGC 
               
               
                   
                   
                 CAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGG 
               
               
                   
                   
                 GATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCG 
               
               
                   
                   
                 GACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTGGCCACTCCCTCTCTGCGCGCTCGCT 
               
               
                   
                   
                 CGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTC 
               
               
                   
                   
                 AGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 278 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 424.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTG 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCTCCAAAACATGAATTGCTGCTGTCCAAAACATGAATTGCTGCTGTCCAAAAC 
               
               
                   
                   
                 ATGAATTGCTGCTGTCCAAAACATGAATTGCTGCTGGTCGACCTCGAGAGATCTACGGGTGG 
               
               
                   
                   
                 CATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCAC 
               
               
                   
                   
                 CAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATAT 
               
               
                   
                   
                 TATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGC 
               
               
                   
                   
                 CTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCT 
               
               
                   
                   
                 CCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCA 
               
               
                   
                   
                 TGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCC 
               
               
                   
                   
                 AGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGG 
               
               
                   
                   
                 ATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCGG 
               
               
                   
                   
                 ACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTC 
               
               
                   
                   
                 GCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCA 
               
               
                   
                   
                 GTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 279 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 122.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTG 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCCAAACACCATTGTCACACTCCACAAACACCATTGTCACACTCCACAAACA 
               
               
                   
                   
                 CCATTGTCACACTCCACAAACACCATTGTCACACTCCAGTCGACCTCGAGAGATCTACGGGT 
               
               
                   
                   
                 GGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCC 
               
               
                   
                   
                 ACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAAT 
               
               
                   
                   
                 ATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGG 
               
               
                   
                   
                 GCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAA 
               
               
                   
                   
                 TCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGG 
               
               
                   
                   
                 CATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGG 
               
               
                   
                   
                 CCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTG 
               
               
                   
                   
                 GGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGC 
               
               
                   
                   
                 GGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCG 
               
               
                   
                   
                 CTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCC 
               
               
                   
                   
                 TCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 280 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 217.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTC 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCTCCAGTCAGTTCCTGATGCAGTATCCAGTCAGTTCCTGATGCAGTATCCAG 
               
               
                   
                   
                 TCAGTTCCTGATGCAGTATCCAGTCAGTTCCTGATGCAGTAGTCGACCTCGAGAGATCTACG 
               
               
                   
                   
                 GGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTG 
               
               
                   
                   
                 CCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTAT 
               
               
                   
                   
                 AATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGT 
               
               
                   
                   
                 AGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTG 
               
               
                   
                   
                 CAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCC 
               
               
                   
                   
                 AGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATA 
               
               
                   
                   
                 TTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATT 
               
               
                   
                   
                 GCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCAC 
               
               
                   
                   
                 GTGCGGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCG 
               
               
                   
                   
                 CTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGC 
               
               
                   
                   
                 GGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 281 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 216A.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTG 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCTCACAGTTGCCAGCTGAGATTATCACAGTTGCCAGCTGAGATTATCACAGT 
               
               
                   
                   
                 TGCCAGCTGAGATTATCACAGTTGCCAGCTGAGATTAGTCGACCTCGAGAGATCTACGGGTG 
               
               
                   
                   
                 GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCA 
               
               
                   
                   
                 CCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATA 
               
               
                   
                   
                 TTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGG 
               
               
                   
                   
                 CCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATC 
               
               
                   
                   
                 TCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGC 
               
               
                   
                   
                 ATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGC 
               
               
                   
                   
                 CAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGG 
               
               
                   
                   
                 GATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCG 
               
               
                   
                   
                 GACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTGGCCACTCCCTCTCTGCGCGCTCGCT 
               
               
                   
                   
                 CGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTC 
               
               
                   
                   
                 AGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 282 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 208A.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTC 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCACAAGCTTTTTGCTCGTCTTATACAAGCTTTTTGCTCGTCTTATACAAGCTT 
               
               
                   
                   
                 TTTGCTCGTCTTATACAAGCTTTTTGCTCGTCTTATGTCGACCTCGAGAGATCTACGGGTGGC 
               
               
                   
                   
                 ATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACC 
               
               
                   
                   
                 AGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATT 
               
               
                   
                   
                 ATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCC 
               
               
                   
                   
                 TGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTC 
               
               
                   
                   
                 CGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCAT 
               
               
                   
                   
                 GCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCC 
               
               
                   
                   
                 AGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGG 
               
               
                   
                   
                 ATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCGG 
               
               
                   
                   
                 ACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTC 
               
               
                   
                   
                 GCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCA 
               
               
                   
                   
                 GTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 283 
                 ITR.Reporter 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 208B.Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGG 
               
               
                   
                   
                 GCCCCCGCGGCATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTC 
               
               
                   
                   
                 ACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTG 
               
               
                   
                   
                 GGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGC 
               
               
                   
                   
                 TGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGC 
               
               
                   
                   
                 CTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTC 
               
               
                   
                   
                 CAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGT 
               
               
                   
                   
                 TCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTG 
               
               
                   
                   
                 AAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCT 
               
               
                   
                   
                 GCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAG 
               
               
                   
                   
                 GGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCG 
               
               
                   
                   
                 TAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTC 
               
               
                   
                   
                 CAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGG 
               
               
                   
                   
                 CGAATTCGCGGATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACA 
               
               
                   
                   
                 CGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTG 
               
               
                   
                   
                 ATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTG 
               
               
                   
                   
                 AGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAAT 
               
               
                   
                   
                 AGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT 
               
               
                   
                   
                 ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGA 
               
               
                   
                   
                 CGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTA 
               
               
                   
                   
                 CGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGA 
               
               
                   
                   
                 CGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTC 
               
               
                   
                   
                 CTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGT 
               
               
                   
                   
                 ACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGAC 
               
               
                   
                   
                 GTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTC 
               
               
                   
                   
                 CGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT 
               
               
                   
                   
                 CGTTTCGTACGTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT 
               
               
                   
                   
                 CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAG 
               
               
                   
                   
                 ATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTG 
               
               
                   
                   
                 ACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
               
               
                   
                   
                 CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGG 
               
               
                   
                   
                 GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGA 
               
               
                   
                   
                 CTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT 
               
               
                   
                   
                 CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTA 
               
               
                   
                   
                 CCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGG 
               
               
                   
                   
                 CCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC 
               
               
                   
                   
                 GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG 
               
               
                   
                   
                 AACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTA 
               
               
                   
                   
                 GACGCGGATCCACAAACCTTTTGTTCGTCTTATACAAACCTTTTGTTCGTCTTATACAAACCT 
               
               
                   
                   
                 TTTGTTCGTCTTATACAAACCTTTTGTTCGTCTTATGTCGACCTCGAGAGATCTACGGGTGGC 
               
               
                   
                   
                 ATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACC 
               
               
                   
                   
                 AGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATT 
               
               
                   
                   
                 ATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCC 
               
               
                   
                   
                 TGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTC 
               
               
                   
                   
                 CGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCAT 
               
               
                   
                   
                 GCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCC 
               
               
                   
                   
                 AGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGG 
               
               
                   
                   
                 ATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCGG 
               
               
                   
                   
                 ACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTC 
               
               
                   
                   
                 GCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCA 
               
               
                   
                   
                 GTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 284 
                 ITR.HCC.V1. 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 Cherry 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCCTAGGCTTCGAATCGATGAATTCGA 
               
               
                   
                   
                 AGCTTCTACCCACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTCAAACTCGAA 
               
               
                   
                   
                 GTCGGCCATATCCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCCGGACCCGGG 
               
               
                   
                   
                 GAATCCCCGTCCCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCATGCGCCATCGC 
               
               
                   
                   
                 CACGTCCTCGCCGTCTAAGTGGAGCTCGTCCCCCAGGCTGACATCGGTCGGGGGGGCCGTCG 
               
               
                   
                   
                 ACAGTCTGCGCGTGTGTCCCGCGGGGAGAAAGGACAGGCGCGGAGCCGCCAGCCCCGCCTC 
               
               
                   
                   
                 TTCGGGGGCGTCGTCGTCCGGGAGATCGAGCAGGCCCTCGATGGTAGACCCGTAATTGTTTT 
               
               
                   
                   
                 TCGTACGCGCGCGGCTGTACGCGGAGGCCTGTTCGACCATCGCGTCGATGCCCGCGACGAG 
               
               
                   
                   
                 CAGGTCGAGGGCGAACTCGAAGTCCCGGTCCAGCATCTCCGCCACGGTGTCGCCGCCCCGG 
               
               
                   
                   
                 GCCGCCATGATGTCCTGCGCGTCCTCGATGACGCCCGCGGTGTCCGGCACCTCGGTCACCGC 
               
               
                   
                   
                 GGTCATCGAGTCCTGGAAGTACTCCTCCGGACTCAGCCCGGTGTCCGCCACCCGGGCGAGG 
               
               
                   
                   
                 AAGCGGCCCTCGATGGTGCCGTAGCCGTAGACGAACTGGAAGACGGCCGAGATGGCGCCGG 
               
               
                   
                   
                 TCAGGCGGTGCGCGGGCAGCCCGCTGCGGCGCACGACGTTCTGCACCGCGCGGGAGAAGGC 
               
               
                   
                   
                 CAGCGAGTGCGGGCCGATGTTGAGGTAGGTGCCGACCAGCCGGGACGACCAGGGGTGGCG 
               
               
                   
                   
                 CACCAGCAGCGCCCGGTTCTCCCGGGCCAGGGCCCGCAGTTCCTCGCGCCAGTCGAGCCCG 
               
               
                   
                   
                 GCGTCCGGGTCCGGGTGGCGCAGCTCGCCGAAGACGGCGTCCAGGGCGAGCTCGAGCAACT 
               
               
                   
                   
                 GGTCCTTGGTGTCGACGTACCAGTACACGGACATCGCGGTGACGTTCAGCTCGGCGGCCAG 
               
               
                   
                   
                 GCGGCGCATCGAGAACCCCGTCAGGCCCTCCGTGTCCAGCAGCCGGACGGTGACCCCGGTG 
               
               
                   
                   
                 ATCCGGTCCCGGTCGAGCCCGGACGGCTGCCCCCCACGGCGACCGCCGCGCCGCCCCTCCCC 
               
               
                   
                   
                 CGACAGCCACACGCTGTCCCGCGGCCCCTCCCGCCCTGCCTTCGCCATGCGCACCTCTCCTC 
               
               
                   
                   
                 GACTCATACCGGTAGCGCTAGCGATGAGCTCTGGTAGTAGACTAGTGGCCCCCATTATATAC 
               
               
                   
                   
                 CCTCTAGAGCATATGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTA 
               
               
                   
                   
                 GTCTCACAAAGAGGGCTTTGTGTAGGGCGCGCCCCCGTAGCTTGGCGTAATCACATGTCCGT 
               
               
                   
                   
                 CGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTGCAAGGCGATTAAGTTGGGTAACGC 
               
               
                   
                   
                 CAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGACATGTGAAATAGCGCTGTACAGCG 
               
               
                   
                   
                 TATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGGCGCGCCAGTTAA 
               
               
                   
                   
                 TAATTAACTAGTTAATAATTAACTAGTTAATAATTAACTCATATGCTCTAGAGGGTATATAA 
               
               
                   
                   
                 TGGGGGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCGCCACCATGGTGAGC 
               
               
                   
                   
                 AAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG 
               
               
                   
                   
                 AGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACG 
               
               
                   
                   
                 AGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGA 
               
               
                   
                   
                 CATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCC 
               
               
                   
                   
                 CCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAG 
               
               
                   
                   
                 GACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTCCAGGACGGCGAGTTCATCTACA 
               
               
                   
                   
                 AGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCAT 
               
               
                   
                   
                 GGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATC 
               
               
                   
                   
                 AAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTAC 
               
               
                   
                   
                 AAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCA 
               
               
                   
                   
                 CCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTG 
               
               
                   
                   
                 CACCGGCGGCATGGACGAGCTGTACAAGTAGGGTACCCAAACACCATTGTCACACTCCAAG 
               
               
                   
                   
                 ATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCAC 
               
               
                   
                   
                 TCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTC 
               
               
                   
                   
                 CTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACA 
               
               
                   
                   
                 ACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGC 
               
               
                   
                   
                 TCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGG 
               
               
                   
                   
                 GATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTC 
               
               
                   
                   
                 ACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCC 
               
               
                   
                   
                 CAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGT 
               
               
                   
                   
                 AACCACGTGCGGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTC 
               
               
                   
                   
                 TGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGC 
               
               
                   
                   
                 CCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 285 
                 ITR.HCC.V1. 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 HSVTK 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCCTAGGCTTCGAATCGATGAATTCGA 
               
               
                   
                   
                 AGCTTCTACCCACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTCAAACTCGAA 
               
               
                   
                   
                 GTCGGCCATATCCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCCGGACCCGGG 
               
               
                   
                   
                 GAATCCCCGTCCCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCATGCGCCATCGC 
               
               
                   
                   
                 CACGTCCTCGCCGTCTAAGTGGAGCTCGTCCCCCAGGCTGACATCGGTCGGGGGGGCCGTCG 
               
               
                   
                   
                 ACAGTCTGCGCGTGTGTCCCGCGGGGAGAAAGGACAGGCGCGGAGCCGCCAGCCCCGCCTC 
               
               
                   
                   
                 TTCGGGGGCGTCGTCGTCCGGGAGATCGAGCAGGCCCTCGATGGTAGACCCGTAATTGTTTT 
               
               
                   
                   
                 TCGTACGCGCGCGGCTGTACGCGGAGGCCTGTTCGACCATCGCGTCGATGCCCGCGACGAG 
               
               
                   
                   
                 CAGGTCGAGGGCGAACTCGAAGTCCCGGTCCAGCATCTCCGCCACGGTGTCGCCGCCCCGG 
               
               
                   
                   
                 GCCGCCATGATGTCCTGCGCGTCCTCGATGACGCCCGCGGTGTCCGGCACCTCGGTCACCGC 
               
               
                   
                   
                 GGTCATCGAGTCCTGGAAGTACTCCTCCGGACTCAGCCCGGTGTCCGCCACCCGGGCGAGG 
               
               
                   
                   
                 AAGCGGCCCTCGATGGTGCCGTAGCCGTAGACGAACTGGAAGACGGCCGAGATGGCGCCGG 
               
               
                   
                   
                 TCAGGCGGTGCGCGGGCAGCCCGCTGCGGCGCACGACGTTCTGCACCGCGCGGGAGAAGGC 
               
               
                   
                   
                 CAGCGAGTGCGGGCCGATGTTGAGGTAGGTGCCGACCAGCCGGGACGACCAGGGGTGGCG 
               
               
                   
                   
                 CACCAGCAGCGCCCGGTTCTCCCGGGCCAGGGCCCGCAGTTCCTCGCGCCAGTCGAGCCCG 
               
               
                   
                   
                 GCGTCCGGGTCCGGGTGGCGCAGCTCGCCGAAGACGGCGTCCAGGGCGAGCTCGAGCAACT 
               
               
                   
                   
                 GGTCCTTGGTGTCGACGTACCAGTACACGGACATCGCGGTGACGTTCAGCTCGGCGGCCAG 
               
               
                   
                   
                 GCGGCGCATCGAGAACCCCGTCAGGCCCTCCGTGTCCAGCAGCCGGACGGTGACCCCGGTG 
               
               
                   
                   
                 ATCCGGTCCCGGTCGAGCCCGGACGGCTGCCCCCCACGGCGACCGCCGCGCCGCCCCTCCCC 
               
               
                   
                   
                 CGACAGCCACACGCTGTCCCGCGGCCCCTCCCGCCCTGCCTTCGCCATGCGCACCTCTCCTC 
               
               
                   
                   
                 GACTCATACCGGTAGCGCTAGCGATGAGCTCTGGTAGTAGACTAGTGGCCCCCATTATATAC 
               
               
                   
                   
                 CCTCTAGAGCATATGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTA 
               
               
                   
                   
                 GTCTCACAAAGAGGGCTTTGTGTAGGGCGCGCCCCCGTAGCTTGGCGTAATCACATGTCCGT 
               
               
                   
                   
                 CGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTGCAAGGCGATTAAGTTGGGTAACGC 
               
               
                   
                   
                 CAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGACATGTGAAATAGCGCTGTACAGCG 
               
               
                   
                   
                 TATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGGCGCGCCAGTTAA 
               
               
                   
                   
                 TAATTAACTAGTTAATAATTAACTAGTTAATAATTAACTCATATGCTCTAGAGGGTATATAA 
               
               
                   
                   
                 TGGGGGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCCGCCACCATGGCTTC 
               
               
                   
                   
                 GTACCCCTGCCATCAACACGCGTCTGCGTTCGACCAGGCTGCGCGTTCTCGCGGCCATAGCA 
               
               
                   
                   
                 ACCGACGTACGGCGTTGCGCCCTCGCCGGCAGCAAGAAGCCACGGAAGTCCGCCTGGAGCA 
               
               
                   
                   
                 GAAAATGCCCACGCTACTGCGGGTTTATATAGACGGTCCTCACGGGATGGGGAAAACCACC 
               
               
                   
                   
                 ACCACGCAACTGCTGGTGGCCCTGGGTTCGCGCGACGATATCGTCTACGTACCCGAGCCGAT 
               
               
                   
                   
                 GACTTACTGGCAGGTGCTGGGGGCTTCCGAGACAATCGCGAACATCTACACCACACAACAC 
               
               
                   
                   
                 CGCCTCGACCAGGGTGAGATATCGGCCGGGGACGCGGCGGTGGTAATGACAAGCGCCCAGA 
               
               
                   
                   
                 TAACAATGGGCATGCCTTATGCCGTGACCGACGCCGTTCTGGCTCCTCATATCGGGGGGGAG 
               
               
                   
                   
                 GCTGGGAGCTCACATGCCCCGCCCCCGGCCCTCACCCTCATCTTCGACCGCCATCCCATCGC 
               
               
                   
                   
                 CGCCCTCCTGTGCTACCCGGCCGCGCGATACCTTATGGGCAGCATGACCCCCCAGGCCGTGC 
               
               
                   
                   
                 TGGCGTTCGTGGCCCTCATCCCGCCGACCTTGCCCGGCACAAACATCGTGTTGGGGGCCCTT 
               
               
                   
                   
                 CCGGAGGACAGACACATCGACCGCCTGGCCAAACGCCAGCGCCCCGGCGAGCGGCTTGACC 
               
               
                   
                   
                 TGGCTATGCTGGCCGCGATTCGCCGCGTTTACGGGCTGCTTGCCAATACGGTGCGGTATCTG 
               
               
                   
                   
                 CAGGGCGGCGGGTCGTGGCGGGAGGATTGGGGACAGCTTTCGGGGACGGCCGTGCCGCCCC 
               
               
                   
                   
                 AGGGTGCCGAGCCCCAGAGCAACGCGGGCCCACGACCCCATATCGGGGACACGTTATTTAC 
               
               
                   
                   
                 CCTGTTTCGGGCCCCCGAGTTGCTGGCCCCCAACGGCGACCTGTACAACGTGTTTGCCTGGG 
               
               
                   
                   
                 CCTTGGACGTCTTGGCCAAACGCCTCCGTCCCATGCACGTCTTTATCCTGGATTACGACCAA 
               
               
                   
                   
                 TCGCCCGCCGGCTGCCGGGACGCCCTGCTGCAACTTACCTCCGGGATGGTCCAGACCCACGT 
               
               
                   
                   
                 CACCACCCCCGGCTCCATACCGACGATCTGCGACCTGGCGCGCACGTTTGCCCGGGAGATG 
               
               
                   
                   
                 GGGGAGGCTAACTGAGGTACCCAAACACCATTGTCACACTCCAAGATCTACGGGTGGCATC 
               
               
                   
                   
                 CCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGC 
               
               
                   
                   
                 CTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATG 
               
               
                   
                   
                 GGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGC 
               
               
                   
                   
                 GGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGC 
               
               
                   
                   
                 CTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCA 
               
               
                   
                   
                 TGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGG 
               
               
                   
                   
                 CTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTA 
               
               
                   
                   
                 CAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTGTAGGTAACCACGTGCGGACC 
               
               
                   
                   
                 GAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCT 
               
               
                   
                   
                 CACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTG 
               
               
                   
                   
                 AGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 286 
                 ITR.HCC.V2. 
                 CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCG 
               
               
                   
                 HSVTK 
                 GGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAAC 
               
               
                   
                   
                 TCCATCACTAGGGGTTCCTGCGGCCGCACGCGTAACTTGTGGACTAAGTTTGTTCACATCCC 
               
               
                   
                   
                 CTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCAC 
               
               
                   
                   
                 ACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAA 
               
               
                   
                   
                 GGCAGGCAGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTG 
               
               
                   
                   
                 GTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATT 
               
               
                   
                   
                 GTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCG 
               
               
                   
                   
                 CACATTTCCCCGAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAAT 
               
               
                   
                   
                 TTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACA 
               
               
                   
                   
                 ATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAA 
               
               
                   
                   
                 AACCTCTACAAATGTGGTATGGCTGATTATGATCCTCCTAGGTGAGGTAGTAGGTTGTATGG 
               
               
                   
                   
                 TTTGAGGTAGTAGGTTGTATGGTTTGAGGTAGTAGGTTGTATGGTTTGAGGTAGTAGGTTGT 
               
               
                   
                   
                 ATGGTTATCGATGAATTCGAAGCTTCTACCCACCGTACTCGTCAATTCCAAGGGCATCGGTA 
               
               
                   
                   
                 AACATCTGCTCAAACTCGAAGTCGGCCATATCCAGAGCGCCGTAGGGGGCGGAGTCGTGGG 
               
               
                   
                   
                 GGGTAAATCCCGGACCCGGGGAATCCCCGTCCCCCAACATGTCCAGATCGAAATCGTCTAG 
               
               
                   
                   
                 CGCGTCGGCATGCGCCATCGCCACGTCCTCGCCGTCTAAGTGGAGCTCGTCCCCCAGGCTGA 
               
               
                   
                   
                 CATCGGTCGGGGGGGCCGTCGACAGTCTGCGCGTGTGTCCCGCGGGGAGAAAGGACAGGCG 
               
               
                   
                   
                 CGGAGCCGCCAGCCCCGCCTCTTCGGGGGCGTCGTCGTCCGGGAGATCGAGCAGGCCCTCG 
               
               
                   
                   
                 ATGGTAGACCCGTAATTGTTTTTCGTACGCGCGCGGCTGTACGCGGAGGCCTGTTCGACCAT 
               
               
                   
                   
                 CGCGTCGATGCCCGCGACGAGCAGGTCGAGGGCGAACTCGAAGTCCCGGTCCAGCATCTCC 
               
               
                   
                   
                 GCCACGGTGTCGCCGCCCCGGGCCGCCATGATGTCCTGCGCGTCCTCGATGACGCCCGCGGT 
               
               
                   
                   
                 GTCCGGCACCTCGGTCACCGCGGTCATCGAGTCCTGGAAGTACTCCTCCGGACTCAGCCCGG 
               
               
                   
                   
                 TGTCCGCCACCCGGGCGAGGAAGCGGCCCTCGATGGTGCCGTAGCCGTAGACGAACTGGAA 
               
               
                   
                   
                 GACGGCCGAGATGGCGCCGGTCAGGCGGTGCGCGGGCAGCCCGCTGCGGCGCACGACGTTC 
               
               
                   
                   
                 TGCACCGCGCGGGAGAAGGCCAGCGAGTGCGGGCCGATGTTGAGGTAGGTGCCGACCAGCC 
               
               
                   
                   
                 GGGACGACCAGGGGTGGCGCACCAGCAGCGCCCGGTTCTCCCGGGCCAGGGCCCGCAGTTC 
               
               
                   
                   
                 CTCGCGCCAGTCGAGCCCGGCGTCCGGGTCCGGGTGGCGCAGCTCGCCGAAGACGGCGTCC 
               
               
                   
                   
                 AGGGCGAGCTCGAGCAACTGGTCCTTGGTGTCGACGTACCAGTACACGGACATCGCGGTGA 
               
               
                   
                   
                 CGTTCAGCTCGGCGGCCAGGCGGCGCATCGAGAACCCCGTCAGGCCCTCCGTGTCCAGCAG 
               
               
                   
                   
                 CCGGACGGTGACCCCGGTGATCCGGTCCCGGTCGAGCCCGGACGGCTGCCCCCCACGGCGA 
               
               
                   
                   
                 CCGCCGCGCCGCCCCTCCCCCGACAGCCACACGCTGTCCCGCGGCCCCTCCCGCCCTGCCTT 
               
               
                   
                   
                 CGCCATGCGCACCTCTCCTCGACTCATACCGGTAGCGCTAGCGATGAGCTCTGGTAGTAGAC 
               
               
                   
                   
                 TAGTGGCCCCCATTATATACCCTCTAGAGCATATGTCTCACAAAGAGGGCTTTGTGTAGTCT 
               
               
                   
                   
                 CACAAAGAGGGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTAGGGCGCGCCCCCGTAGC 
               
               
                   
                   
                 TTGGCGTAATCACATGTCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTGCAAG 
               
               
                   
                   
                 GCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGACATG 
               
               
                   
                   
                 TGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACA 
               
               
                   
                   
                 CCGTACGGCGCGCCAGTTAATAATTAACTAGTTAATAATTAACTAGTTAATAATTAACTCAT 
               
               
                   
                   
                 ATGCTCTAGAGGGTATATAATGGGGGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTG 
               
               
                   
                   
                 GATCCCGCCACCATGGCTTCGTACCCCTGCCATCAACACGCGTCTGCGTTCGACCAGGCTGC 
               
               
                   
                   
                 GCGTTCTCGCGGCCATAGCAACCGACGTACGGCGTTGCGCCCTCGCCGGCAGCAAGAAGCC 
               
               
                   
                   
                 ACGGAAGTCCGCCTGGAGCAGAAAATGCCCACGCTACTGCGGGTTTATATAGACGGTCCTC 
               
               
                   
                   
                 ACGGGATGGGGAAAACCACCACCACGCAACTGCTGGTGGCCCTGGGTTCGCGCGACGATAT 
               
               
                   
                   
                 CGTCTACGTACCCGAGCCGATGACTTACTGGCAGGTGCTGGGGGCTTCCGAGACAATCGCG 
               
               
                   
                   
                 AACATCTACACCACACAACACCGCCTCGACCAGGGTGAGATATCGGCCGGGGACGCGGCGG 
               
               
                   
                   
                 TGGTAATGACAAGCGCCCAGATAACAATGGGCATGCCTTATGCCGTGACCGACGCCGTTCT 
               
               
                   
                   
                 GGCTCCTCATATCGGGGGGGAGGCTGGGAGCTCACATGCCCCGCCCCCGGCCCTCACCCTCA 
               
               
                   
                   
                 TCTTCGACCGCCATCCCATCGCCGCCCTCCTGTGCTACCCGGCCGCGCGATACCTTATGGGC 
               
               
                   
                   
                 AGCATGACCCCCCAGGCCGTGCTGGCGTTCGTGGCCCTCATCCCGCCGACCTTGCCCGGCAC 
               
               
                   
                   
                 AAACATCGTGTTGGGGGCCCTTCCGGAGGACAGACACATCGACCGCCTGGCCAAACGCCAG 
               
               
                   
                   
                 CGCCCCGGCGAGCGGCTTGACCTGGCTATGCTGGCCGCGATTCGCCGCGTTTACGGGCTGCT 
               
               
                   
                   
                 TGCCAATACGGTGCGGTATCTGCAGGGCGGCGGGTCGTGGCGGGAGGATTGGGGACAGCTT 
               
               
                   
                   
                 TCGGGGACGGCCGTGCCGCCCCAGGGTGCCGAGCCCCAGAGCAACGCGGGCCCACGACCCC 
               
               
                   
                   
                 ATATCGGGGACACGTTATTTACCCTGTTTCGGGCCCCCGAGTTGCTGGCCCCCAACGGCGAC 
               
               
                   
                   
                 CTGTACAACGTGTTTGCCTGGGCCTTGGACGTCTTGGCCAAACGCCTCCGTCCCATGCACGT 
               
               
                   
                   
                 CTTTATCCTGGATTACGACCAATCGCCCGCCGGCTGCCGGGACGCCCTGCTGCAACTTACCT 
               
               
                   
                   
                 CCGGGATGGTCCAGACCCACGTCACCACCCCCGGCTCCATACCGACGATCTGCGACCTGGC 
               
               
                   
                   
                 GCGCACGTTTGCCCGGGAGATGGGGGAGGCTAACTGAGGTACCAACCATACAACCTACTAC 
               
               
                   
                   
                 CTCAAACCATACAACCTACTACCTCAAACCATACAACCTACTACCTCAAACCATACAACCTA 
               
               
                   
                   
                 CTACCTCAAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGG 
               
               
                   
                   
                 AAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTG 
               
               
                   
                   
                 ACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGT 
               
               
                   
                   
                 TGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCA 
               
               
                   
                   
                 CAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCC 
               
               
                   
                   
                 GAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAG 
               
               
                   
                   
                 ACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCAC 
               
               
                   
                   
                 CTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGA 
               
               
                   
                   
                 TTTTGTAGGTAACCACGTGCGGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCC 
               
               
                   
                   
                 ACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCC 
               
               
                   
                   
                 GGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG 
               
               
                   
               
               
                 287 
                 C.CMV.Cherry 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                   
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCAAGCACTCTGATTTGACAATTAAAGCACTCTGATTTGACAATTAAAGCACTCTGATTTGA 
               
               
                   
                   
                 CAATTAAAGCACTCTGATTTGACAATTAGTCGACCTCGAGAGATCTACGGGTGGCATCCCTG 
               
               
                   
                   
                 TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG 
               
               
                   
                   
                 TCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGT 
               
               
                   
                   
                 GGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGG 
               
               
                   
                   
                 TCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCC 
               
               
                   
                   
                 TGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGAC 
               
               
                   
                   
                 CAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGT 
               
               
                   
                   
                 CTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGG 
               
               
                   
                   
                 CGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 288 
                 c.Letc.Cherry 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                   
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCAACCATACAACCTACTACCTCAAACCATACAACCTACTACCTCAAACCATACAACCTACT 
               
               
                   
                   
                 ACCTCAAACCATACAACCTACTACCTCAGTCGACCTCGAGAGATCTACGGGTGGCATCCCTG 
               
               
                   
                   
                 TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG 
               
               
                   
                   
                 TCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGT 
               
               
                   
                   
                 GGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGG 
               
               
                   
                   
                 TCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCC 
               
               
                   
                   
                 TGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGAC 
               
               
                   
                   
                 CAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGT 
               
               
                   
                   
                 CTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGG 
               
               
                   
                   
                 CGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 289 
                 C.TF-AND. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCCTAGGCTTCGAATCGATGAATTCGAAGCTTCTACCC 
               
               
                   
                   
                 ACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTCAAACTCGAAGTCGGCCATAT 
               
               
                   
                   
                 CCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCCGGACCCGGGGAATCCCCGTC 
               
               
                   
                   
                 CCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCATGCGCCATCGCCACGTCCTCGC 
               
               
                   
                   
                 CGTCTAAGTGGAGCTCGTCCCCCAGGCTGACATCGGTCGGGGGGGCCGTCGACAGTCTGCG 
               
               
                   
                   
                 CGTGTGTCCCGCGGGGAGAAAGGACAGGCGCGGAGCCGCCAGCCCCGCCTCTTCGGGGGCG 
               
               
                   
                   
                 TCGTCGTCCGGGAGATCGAGCAGGCCCTCGATGGTAGACCCGTAATTGTTTTTCGTACGCGC 
               
               
                   
                   
                 GCGGCTGTACGCGGAGGCCTGTTCGACCATCGCGTCGATGCCCGCGACGAGCAGGTCGAGG 
               
               
                   
                   
                 GCGAACTCGAAGTCCCGGTCCAGCATCTCCGCCACGGTGTCGCCGCCCCGGGCCGCCATGAT 
               
               
                   
                   
                 GTCCTGCGCGTCCTCGATGACGCCCGCGGTGTCCGGCACCTCGGTCACCGCGGTCATCGAGT 
               
               
                   
                   
                 CCTGGAAGTACTCCTCCGGACTCAGCCCGGTGTCCGCCACCCGGGCGAGGAAGCGGCCCTC 
               
               
                   
                   
                 GATGGTGCCGTAGCCGTAGACGAACTGGAAGACGGCCGAGATGGCGCCGGTCAGGCGGTGC 
               
               
                   
                   
                 GCGGGCAGCCCGCTGCGGCGCACGACGTTCTGCACCGCGCGGGAGAAGGCCAGCGAGTGCG 
               
               
                   
                   
                 GGCCGATGTTGAGGTAGGTGCCGACCAGCCGGGACGACCAGGGGTGGCGCACCAGCAGCG 
               
               
                   
                   
                 CCCGGTTCTCCCGGGCCAGGGCCCGCAGTTCCTCGCGCCAGTCGAGCCCGGCGTCCGGGTCC 
               
               
                   
                   
                 GGGTGGCGCAGCTCGCCGAAGACGGCGTCCAGGGCGAGCTCGAGCAACTGGTCCTTGGTGT 
               
               
                   
                   
                 CGACGTACCAGTACACGGACATCGCGGTGACGTTCAGCTCGGCGGCCAGGCGGCGCATCGA 
               
               
                   
                   
                 GAACCCCGTCAGGCCCTCCGTGTCCAGCAGCCGGACGGTGACCCCGGTGATCCGGTCCCGG 
               
               
                   
                   
                 TCGAGCCCGGACGGCTGCCCCCCACGGCGACCGCCGCGCCGCCCCTCCCCCGACAGCCACA 
               
               
                   
                   
                 CGCTGTCCCGCGGCCCCTCCCGCCCTGCCTTCGCCATGCGCACCTCTCCTCGACTCATACCGG 
               
               
                   
                   
                 TAGCGCTAGCGATGAGCTCTGGTAGTAGACTAGTGGCCCCCATTATATACCCTCTAGAGCAT 
               
               
                   
                   
                 ATGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGA 
               
               
                   
                   
                 GGGCTTTGTGTAGGGCGCGCCCCCGTAGCTTGGCGTAATCACATGTCCGTCGTTTTACAACG 
               
               
                   
                   
                 TCGTGACTGGGAAAACCCTGGCCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCC 
               
               
                   
                   
                 AGTCACGACGTTGTAAAACGACGGACATGTGAAATAGCGCTGTACAGCGTATGGGAATCTC 
               
               
                   
                   
                 TTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGGCGCGCCAGTTAATAATTAACTAGT 
               
               
                   
                   
                 TAATAATTAACTAGTTAATAATTAACTCATATGCTCTAGAGGGTATATAATGGGGGCCACTA 
               
               
                   
                   
                 GTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCGCCACCATGGTGAGCAAGGGCGAGGA 
               
               
                   
                   
                 GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTG 
               
               
                   
                   
                 AACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAG 
               
               
                   
                   
                 ACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCC 
               
               
                   
                   
                 TCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGA 
               
               
                   
                   
                 AGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGT 
               
               
                   
                   
                 GGTGACCGTGACCCAGGACTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTG 
               
               
                   
                   
                 CGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGG 
               
               
                   
                   
                 CCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCT 
               
               
                   
                   
                 GAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAA 
               
               
                   
                   
                 GCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAAC 
               
               
                   
                   
                 GAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCA 
               
               
                   
                   
                 TGGACGAGCTGTACAAGTAGGGTACCGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGT 
               
               
                   
                   
                 GACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGT 
               
               
                   
                   
                 CCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTG 
               
               
                   
                   
                 GAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGT 
               
               
                   
                   
                 CTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCT 
               
               
                   
                   
                 GGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACC 
               
               
                   
                   
                 AGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTC 
               
               
                   
                   
                 TCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGC 
               
               
                   
                   
                 GTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 290 
                 HCC.V2.Cherry 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                   
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCCTAGGTGAGGTAGTAGGTTGTATGGTTTGAGGTAGT 
               
               
                   
                   
                 AGGTTGTATGGTTTGAGGTAGTAGGTTGTATGGTTTGAGGTAGTAGGTTGTATGGTTATCGA 
               
               
                   
                   
                 TGAATTCGAAGCTTCTACCCACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTC 
               
               
                   
                   
                 AAACTCGAAGTCGGCCATATCCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCC 
               
               
                   
                   
                 GGACCCGGGGAATCCCCGTCCCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCAT 
               
               
                   
                   
                 GCGCCATCGCCACGTCCTCGCCGTCTAAGTGGAGCTCGTCCCCCAGGCTGACATCGGTCGGG 
               
               
                   
                   
                 GGGGCCGTCGACAGTCTGCGCGTGTGTCCCGCGGGGAGAAAGGACAGGCGCGGAGCCGCC 
               
               
                   
                   
                 AGCCCCGCCTCTTCGGGGGCGTCGTCGTCCGGGAGATCGAGCAGGCCCTCGATGGTAGACC 
               
               
                   
                   
                 CGTAATTGTTTTTCGTACGCGCGCGGCTGTACGCGGAGGCCTGTTCGACCATCGCGTCGATG 
               
               
                   
                   
                 CCCGCGACGAGCAGGTCGAGGGCGAACTCGAAGTCCCGGTCCAGCATCTCCGCCACGGTGT 
               
               
                   
                   
                 CGCCGCCCCGGGCCGCCATGATGTCCTGCGCGTCCTCGATGACGCCCGCGGTGTCCGGCACC 
               
               
                   
                   
                 TCGGTCACCGCGGTCATCGAGTCCTGGAAGTACTCCTCCGGACTCAGCCCGGTGTCCGCCAC 
               
               
                   
                   
                 CCGGGCGAGGAAGCGGCCCTCGATGGTGCCGTAGCCGTAGACGAACTGGAAGACGGCCGA 
               
               
                   
                   
                 GATGGCGCCGGTCAGGCGGTGCGCGGGCAGCCCGCTGCGGCGCACGACGTTCTGCACCGCG 
               
               
                   
                   
                 CGGGAGAAGGCCAGCGAGTGCGGGCCGATGTTGAGGTAGGTGCCGACCAGCCGGGACGAC 
               
               
                   
                   
                 CAGGGGTGGCGCACCAGCAGCGCCCGGTTCTCCCGGGCCAGGGCCCGCAGTTCCTCGCGCC 
               
               
                   
                   
                 AGTCGAGCCCGGCGTCCGGGTCCGGGTGGCGCAGCTCGCCGAAGACGGCGTCCAGGGCGAG 
               
               
                   
                   
                 CTCGAGCAACTGGTCCTTGGTGTCGACGTACCAGTACACGGACATCGCGGTGACGTTCAGCT 
               
               
                   
                   
                 CGGCGGCCAGGCGGCGCATCGAGAACCCCGTCAGGCCCTCCGTGTCCAGCAGCCGGACGGT 
               
               
                   
                   
                 GACCCCGGTGATCCGGTCCCGGTCGAGCCCGGACGGCTGCCCCCCACGGCGACCGCCGCGC 
               
               
                   
                   
                 CGCCCCTCCCCCGACAGCCACACGCTGTCCCGCGGCCCCTCCCGCCCTGCCTTCGCCATGCG 
               
               
                   
                   
                 CACCTCTCCTCGACTCATACCGGTAGCGCTAGCGATGAGCTCTGGTAGTAGACTAGTGGCCC 
               
               
                   
                   
                 CCATTATATACCCTCTAGAGCATATGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGAG 
               
               
                   
                   
                 GGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTAGGGCGCGCCCCCGTAGCTTGGCGTAAT 
               
               
                   
                   
                 CACATGTCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTGCAAGGCGATTAAGT 
               
               
                   
                   
                 TGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGACATGTGAAATAGCG 
               
               
                   
                   
                 CTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGGCG 
               
               
                   
                   
                 CGCCAGTTAATAATTAACTAGTTAATAATTAACTAGTTAATAATTAACTCATATGCTCTAGA 
               
               
                   
                   
                 GGGTATATAATGGGGGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCGCCAC 
               
               
                   
                   
                 CATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAG 
               
               
                   
                   
                 GTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGC 
               
               
                   
                   
                 CGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCT 
               
               
                   
                   
                 TCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCC 
               
               
                   
                   
                 GCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGAT 
               
               
                   
                   
                 GAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTCCAGGACGGCGAG 
               
               
                   
                   
                 TTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAA 
               
               
                   
                   
                 GAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAG 
               
               
                   
                   
                 GGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAG 
               
               
                   
                   
                 ACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGT 
               
               
                   
                   
                 TGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGG 
               
               
                   
                   
                 CCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGGGTACCAACCATACAACCTAC 
               
               
                   
                   
                 TACCTCAAACCATACAACCTACTACCTCAAACCATACAACCTACTACCTCAAACCATACAAC 
               
               
                   
                   
                 CTACTACCTCAAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCT&#39;CCTGGCCCT 
               
               
                   
                   
                 GGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGT 
               
               
                   
                   
                 CTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCA 
               
               
                   
                   
                 AGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTG 
               
               
                   
                   
                 GCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCT 
               
               
                   
                   
                 CCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAG 
               
               
                   
                   
                 AGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCC 
               
               
                   
                   
                 ACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 291 
                 C.HNF1-FB. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCCTAGGGGGTCCACTTGCTCCTGGGCCCACACAGTCC 
               
               
                   
                   
                 TGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGC 
               
               
                   
                   
                 AGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCAT 
               
               
                   
                   
                 GGGGACCTGGATGCTGTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCG 
               
               
                   
                   
                 GTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAA 
               
               
                   
                   
                 GTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGAATTCGAAGCTTAC 
               
               
                   
                   
                 GACGGACATGTGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTAT 
               
               
                   
                   
                 CTTCCCGTACACCGTACGGCGCGCCAGTTAATAATTAACTAGTTAATAATTAACTAGTTAAT 
               
               
                   
                   
                 AATTAACTCATATGCTCTAGAGGGTATATAATGGGGGCCACTAGTCTACTACCAGAGCTCAT 
               
               
                   
                   
                 CGCTAGCGCTGGATCCGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATC 
               
               
                   
                   
                 AAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGA 
               
               
                   
                   
                 TCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGA 
               
               
                   
                   
                 CCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCC 
               
               
                   
                   
                 AAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGG 
               
               
                   
                   
                 CTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGAC 
               
               
                   
                   
                 TCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTC 
               
               
                   
                   
                 CGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTAC 
               
               
                   
                   
                 CCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGC 
               
               
                   
                   
                 CACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCG 
               
               
                   
                   
                 CCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGA 
               
               
                   
                   
                 ACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTCC 
               
               
                   
                   
                 GGAAGAGCCGAGGGCAGGGGAAGTCTTCTAACATGCGGGGACGTGGAGGAAAATCCCGGG 
               
               
                   
                   
                 CCCAGATCTATGAGTCGAGGAGAGGTGCGCATGGCGAAGGCAGGGCGGGAGGGGCCGCGG 
               
               
                   
                   
                 GACAGCGTGTGGCTGTCGGGGGAGGGGCGGCGCGGCGGTCGCCGTGGGGGGCAGCCGTCC 
               
               
                   
                   
                 GGGCTCGACCGGGACCGGATCACCGGGGTCACCGTCCGGCTGCTGGACACGGAGGGCCTGA 
               
               
                   
                   
                 CGGGGTTCTCGATGCGCCGCCTGGCCGCCGAGCTGAACGTCACCGCGATGTCCGTGTACTGG 
               
               
                   
                   
                 TACGTCGACACCAAGGACCAGTTGCTCGAGCTCGCCCTGGACGCCGTCTTCGGCGAGCTGCG 
               
               
                   
                   
                 CCACCCGGACCCGGACGCCGGGCTCGACTGGCGCGAGGAACTGCGGGCCCTGGCCCGGGAG 
               
               
                   
                   
                 AACCGGGCGCTGCTGGTGCGCCACCCCTGGTCGTCCCGGCTGGTCGGCACCTACCTCAACAT 
               
               
                   
                   
                 CGGCCCGCACTCGCTGGCCTTCTCCCGCGCGGTGCAGAACGTCGTGCGCCGCAGCGGGCTGC 
               
               
                   
                   
                 CCGCGCACCGCCTGACCGGCGCCATCTCGGCCGTCTTCCAGTTCGTCTACGGCTACGGCACC 
               
               
                   
                   
                 ATCGAGGGCCGCTTCCTCGCCCGGGTGGCGGACACCGGGCTGAGTCCGGAGGAGTACTTCC 
               
               
                   
                   
                 AGGACTCGATGACCGCGGTGACCGAGGTGCCGGACACCGCGGGCGTCATCGAGGACGCGCA 
               
               
                   
                   
                 GGACATCATGGCGGCCCGGGGCGGCGACACCGTGGCGGAGATGCTGGACCGGGACTTCGAG 
               
               
                   
                   
                 TTCGCCCTCGACCTGCTCGTCGCGGGCATCGACGCGATGGTCGAACAGGCCTCCGCGTACAG 
               
               
                   
                   
                 CCGCGCGCATGATGAGTTTCCCACCATGGTGTTTCCTTCTGGGCAGATCAGCCAGGCCTCGG 
               
               
                   
                   
                 CCTTGGCCCCGGCCCCTCCCCAAGTCCTGCCCCAGGCTCCAGCCCCTGCCCCTGCTCCAGCC 
               
               
                   
                   
                 ATGGTATCAGCTCTGGCCCAGGCCCCAGCCCCTGTCCCAGTCCTAGCCCCAGGCCCTCCTCA 
               
               
                   
                   
                 GGCTGTGGCCCCACCTGCCCCCAAGCCCACCCAGGCTGGGGAAGGAACGCTGTCAGAGGCC 
               
               
                   
                   
                 CTGCTGCAGCTGCAGTTTGATGATGAAGACCTGGGGGCCTTGCTTGGCAACAGCACAGACC 
               
               
                   
                   
                 CAGCTGTGTTCACAGACCTGGCATCCGTCGACAACTCCGAGTTTCAGCAGCTGCTGAACCAG 
               
               
                   
                   
                 GGCATACCTGTGGCCCCCCACACAACTGAGCCCATGCTGATGGAGTACCCTGAGGCTATAA 
               
               
                   
                   
                 CTCGCCTAGTGACAGGGGCCCAGAGGCCCCCCGACCCAGCTCCTGCTCCACTGGGGGCCCC 
               
               
                   
                   
                 GGGGCTCCCCAATGGCCTCCTTTCAGGAGATGAAGACTTCTCCTCCATTGCGGACATGGACT 
               
               
                   
                   
                 TCTCAGCCCTGCTGAGTCAGATCAGCTCCTAAGGAAGCTTGGTACCGTCGACCTCGAGAGAT 
               
               
                   
                   
                 CTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTC 
               
               
                   
                   
                 CAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCT 
               
               
                   
                   
                 TCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAAC 
               
               
                   
                   
                 CTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTC 
               
               
                   
                   
                 ACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGG 
               
               
                   
                   
                 ATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCA 
               
               
                   
                   
                 CCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCC 
               
               
                   
                   
                 AAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 292 
                 C.SOX-FB. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCCTAGGGGGTCCACTTGCTCCTGGGCCCACACAGTCC 
               
               
                   
                   
                 TGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGC 
               
               
                   
                   
                 AGGTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCAT 
               
               
                   
                   
                 GGGGACCTGGATGCTGTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCG 
               
               
                   
                   
                 GTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAA 
               
               
                   
                   
                 GTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGAATTCGAAGCTTAC 
               
               
                   
                   
                 GACGGACATGTGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTAT 
               
               
                   
                   
                 CTTCCCGTACACCGTACGGCGCGCCCTACACAAAGCCCTCTTTGTGAGACTACACAAAGCCC 
               
               
                   
                   
                 TCTTTGTGAGACTACACAAAGCCCTCTTTGTGAGACATATGCTCTAGAGGGTATATAATGGG 
               
               
                   
                   
                 GGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCGCCACCATGGTGAGCAAG 
               
               
                   
                   
                 GGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGG 
               
               
                   
                   
                 GCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGG 
               
               
                   
                   
                 GCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACAT 
               
               
                   
                   
                 CCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCG 
               
               
                   
                   
                 ACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGAC 
               
               
                   
                   
                 GGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGG 
               
               
                   
                   
                 TGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGG 
               
               
                   
                   
                 CTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAG 
               
               
                   
                   
                 CAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAG 
               
               
                   
                   
                 GCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCT 
               
               
                   
                   
                 CCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCAC 
               
               
                   
                   
                 CGGCGGCATGGACGAGCTGTACAAGTCCGGAAGAGCCGAGGGCAGGGGAAGTCTTCTAAC 
               
               
                   
                   
                 ATGCGGGGACGTGGAGGAAAATCCCGGGCCCAGATCTATGAGTCGAGGAGAGGTGCGCAT 
               
               
                   
                   
                 GGCGAAGGCAGGGCGGGAGGGGCCGCGGGACAGCGTGTGGCTGTCGGGGGAGGGGCGGCG 
               
               
                   
                   
                 CGGCGGTCGCCGTGGGGGGCAGCCGTCCGGGCTCGACCGGGACCGGATCACCGGGGTCACC 
               
               
                   
                   
                 GTCCGGCTGCTGGACACGGAGGGCCTGACGGGGTTCTCGATGCGCCGCCTGGCCGCCGAGC 
               
               
                   
                   
                 TGAACGTCACCGCGATGTCCGTGTACTGGTACGTCGACACCAAGGACCAGTTGCTCGAGCTC 
               
               
                   
                   
                 GCCCTGGACGCCGTCTTCGGCGAGCTGCGCCACCCGGACCCGGACGCCGGGCTCGACTGGC 
               
               
                   
                   
                 GCGAGGAACTGCGGGCCCTGGCCCGGGAGAACCGGGCGCTGCTGGTGCGCCACCCCTGGTC 
               
               
                   
                   
                 GTCCCGGCTGGTCGGCACCTACCTCAACATCGGCCCGCACTCGCTGGCCTTCTCCCGCGCGG 
               
               
                   
                   
                 TGCAGAACGTCGTGCGCCGCAGCGGGCTGCCCGCGCACCGCCTGACCGGCGCCATCTCGGC 
               
               
                   
                   
                 CGTCTTCCAGTTCGTCTACGGCTACGGCACCATCGAGGGCCGCTTCCTCGCCCGGGTGGCGG 
               
               
                   
                   
                 ACACCGGGCTGAGTCCGGAGGAGTACTTCCAGGACTCGATGACCGCGGTGACCGAGGTGCC 
               
               
                   
                   
                 GGACACCGCGGGCGTCATCGAGGACGCGCAGGACATCATGGCGGCCCGGGGCGGCGACAC 
               
               
                   
                   
                 CGTGGCGGAGATGCTGGACCGGGACTTCGAGTTCGCCCTCGACCTGCTCGTCGCGGGCATCG 
               
               
                   
                   
                 ACGCGATGGTCGAACAGGCCTCCGCGTACAGCCGCGCGCATGATGAGTTTCCCACCATGGT 
               
               
                   
                   
                 GTTTCCTTCTGGGCAGATCAGCCAGGCCTCGGCCTTGGCCCCGGCCCCTCCCCAAGTCCTGC 
               
               
                   
                   
                 CCCAGGCTCCAGCCCCTGCCCCTGCTCCAGCCATGGTATCAGCTCTGGCCCAGGCCCCAGCC 
               
               
                   
                   
                 CCTGTCCCAGTCCTAGCCCCAGGCCCTCCTCAGGCTGTGGCCCCACCTGCCCCCAAGCCCAC 
               
               
                   
                   
                 CCAGGCTGGGGAAGGAACGCTGTCAGAGGCCCTGCTGCAGCTGCAGTTTGATGATGAAGAC 
               
               
                   
                   
                 CTGGGGGCCTTGCTTGGCAACAGCACAGACCCAGCTGTGTTCACAGACCTGGCATCCGTCGA 
               
               
                   
                   
                 CAACTCCGAGTTTCAGCAGCTGCTGAACCAGGGCATACCTGTGGCCCCCCACACAACTGAG 
               
               
                   
                   
                 CCCATGCTGATGGAGTACCCTGAGGCTATAACTCGCCTAGTGACAGGGGCCCAGAGGCCCC 
               
               
                   
                   
                 CCGACCCAGCTCCTGCTCCACTGGGGGCCCCGGGGCTCCCCAATGGCCTCCTTTCAGGAGAT 
               
               
                   
                   
                 GAAGACTTCTCCTCCATTGCGGACATGGACTTCTCAGCCCTGCTGAGTCAGATCAGCTCCTA 
               
               
                   
                   
                 AGGAAGCTTGGTACCGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCA 
               
               
                   
                   
                 GTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATT 
               
               
                   
                   
                 AAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGG 
               
               
                   
                   
                 TATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACC 
               
               
                   
                   
                 AAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGA 
               
               
                   
                   
                 TTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAA 
               
               
                   
                   
                 TTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAA 
               
               
                   
                   
                 TCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGC 
               
               
                   
                   
                 TCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 293 
                 Reporter 26. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCACCTATCCTGAATTACTTGAAACCTATCCTGAATTACTTGAAACCTATCCTGAATTACTTG 
               
               
                   
                   
                 AAACCTATCCTGAATTACTTGAAGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGTGACC 
               
               
                   
                   
                 CCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTA 
               
               
                   
                   
                 ATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAG 
               
               
                   
                   
                 GGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTAT 
               
               
                   
                   
                 TGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGT 
               
               
                   
                   
                 TCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGC 
               
               
                   
                   
                 TCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCA 
               
               
                   
                   
                 ACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGA 
               
               
                   
                   
                 ACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 294 
                 Repoter 22. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCACAGTTCTTCAACTGGCAGCTTACAGTTCTTCAACTGGCAGCTTACAGTTCTTCAACTGGC 
               
               
                   
                   
                 AGCTTACAGTTCTTCAACTGGCAGCTTGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGT 
               
               
                   
                   
                 GACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGT 
               
               
                   
                   
                 CCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTG 
               
               
                   
                   
                 GAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGT 
               
               
                   
                   
                 CTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCT 
               
               
                   
                   
                 GGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACC 
               
               
                   
                   
                 AGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTC 
               
               
                   
                   
                 TCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGC 
               
               
                   
                   
                 GTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 295 
                 Reporter 126. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCCGTGTTCACAGCGGACCTTGATCGTGTTCACAGCGGACCTTGATCGTGTTCACAGCGGAC 
               
               
                   
                   
                 CTTGATCGTGTTCACAGCGGACCTTGATGTCGACCTCGAGAGATCTACGGGTGGCATCCCTG 
               
               
                   
                   
                 TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG 
               
               
                   
                   
                 TCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGT 
               
               
                   
                   
                 GGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGG 
               
               
                   
                   
                 TCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCC 
               
               
                   
                   
                 TGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGAC 
               
               
                   
                   
                 CAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGT 
               
               
                   
                   
                 CTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGG 
               
               
                   
                   
                 CGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 296 
                 Reporter 424. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CTCCAAAACATGAATTGCTGCTGTCCAAAACATGAATTGCTGCTGTCCAAAACATGAATTGC 
               
               
                   
                   
                 TGCTGTCCAAAACATGAATTGCTGCTGGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGT 
               
               
                   
                   
                 GACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGT 
               
               
                   
                   
                 CCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTG 
               
               
                   
                   
                 GAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGT 
               
               
                   
                   
                 CTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCT 
               
               
                   
                   
                 GGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACC 
               
               
                   
                   
                 AGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTC 
               
               
                   
                   
                 TCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGC 
               
               
                   
                   
                 GTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 297 
                 Reporter 122. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCCAAACACCATTGTCACACTCCACAAACACCATTGTCACACTCCACAAACACCATTGTCAC 
               
               
                   
                   
                 ACTCCACAAACACCATTGTCACACTCCAGTCGACCTCGAGAGATCTACGGGTGGCATCCCTG 
               
               
                   
                   
                 TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG 
               
               
                   
                   
                 TCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGT 
               
               
                   
                   
                 GGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGG 
               
               
                   
                   
                 TCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCC 
               
               
                   
                   
                 TGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGAC 
               
               
                   
                   
                 CAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGT 
               
               
                   
                   
                 CTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGG 
               
               
                   
                   
                 CGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 298 
                 Reporter 217. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCTCCAGTCAGTTCCTGATGCAGTATCCAGTCAGTTCCTGATGCAGTATCCAGTCAGTTCCTG 
               
               
                   
                   
                 ATGCAGTATCCAGTCAGTTCCTGATGCAGTAGTCGACCTCGAGAGATCTACGGGTGGCATCC 
               
               
                   
                   
                 CTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCC 
               
               
                   
                   
                 TTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGG 
               
               
                   
                   
                 GGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCG 
               
               
                   
                   
                 GGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCC 
               
               
                   
                   
                 TCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCAT 
               
               
                   
                   
                 GACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGC 
               
               
                   
                   
                 TGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTAC 
               
               
                   
                   
                 AGGCGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 299 
                 Reporter 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 216A.Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCTCACAGTTGCCAGCTGAGATTATCACAGTTGCCAGCTGAGATTATCACAGTTGCCAGCTG 
               
               
                   
                   
                 AGATTATCACAGTTGCCAGCTGAGATTAGTCGACCTCGAGAGATCTACGGGTGGCATCCCTG 
               
               
                   
                   
                 TGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG 
               
               
                   
                   
                 TCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGT 
               
               
                   
                   
                 GGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGG 
               
               
                   
                   
                 TCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCC 
               
               
                   
                   
                 TGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGAC 
               
               
                   
                   
                 CAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGT 
               
               
                   
                   
                 CTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGG 
               
               
                   
                   
                 CGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 300 
                 Reporter 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 208A.Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCACAAGCTTTTTGCTCGTCTTATACAAGCTTTTTGCTCGTCTTATACAAGCTTTTTGCTCGTC 
               
               
                   
                   
                 TTATACAAGCTTTTTGCTCGTCTTATGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGTGA 
               
               
                   
                   
                 CCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCT 
               
               
                   
                   
                 AATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGA 
               
               
                   
                   
                 GGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCT 
               
               
                   
                   
                 ATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGG 
               
               
                   
                   
                 GTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAG 
               
               
                   
                   
                 GCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTC 
               
               
                   
                   
                 CAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGT 
               
               
                   
                   
                 GAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 301 
                 Reporter 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 208B.Cherry 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCTAGACTGCAGCCTCAGGAGATCTGGGCCCCCGCGGC 
               
               
                   
                   
                 ATATGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAG 
               
               
                   
                   
                 CAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGGTGCTCAGGT 
               
               
                   
                   
                 AGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTC 
               
               
                   
                   
                 GGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGT 
               
               
                   
                   
                 TCTTCTGCTTGTCGGCGGTGATATAGACGTTGTCGCTGATGGCGTTGTACTCCAGCTTGTGCC 
               
               
                   
                   
                 CCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTG 
               
               
                   
                   
                 TCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGT 
               
               
                   
                   
                 GCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGT 
               
               
                   
                   
                 CGGGGTAGCGGGCGAAGCACTGCACGCCCCAGGTCAGGGTGGTCACGAGGGTGGGCCAGG 
               
               
                   
                   
                 GCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATC 
               
               
                   
                   
                 GCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCA 
               
               
                   
                   
                 GGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCGAATTCGCGG 
               
               
                   
                   
                 ATCTGACGGTTCACTAAACCAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCC 
               
               
                   
                   
                 CATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCC 
               
               
                   
                   
                 AAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCG 
               
               
                   
                   
                 CTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACACTAGTTATTAATAGTAATCAAT 
               
               
                   
                   
                 TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATG 
               
               
                   
                   
                 GCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCC 
               
               
                   
                   
                 ATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC 
               
               
                   
                   
                 CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACG 
               
               
                   
                   
                 GTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAG 
               
               
                   
                   
                 TACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGG 
               
               
                   
                   
                 GCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGA 
               
               
                   
                   
                 GTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTG 
               
               
                   
                   
                 ACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTCGTAC 
               
               
                   
                   
                 GTTCGAAGCCACCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTC 
               
               
                   
                   
                 ATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCG 
               
               
                   
                   
                 AGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTG 
               
               
                   
                   
                 GCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC 
               
               
                   
                   
                 GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG 
               
               
                   
                   
                 GGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTC 
               
               
                   
                   
                 CAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCC 
               
               
                   
                   
                 CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGA 
               
               
                   
                   
                 CGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCTGAAGCTGAAGGACGGCGGCCACTACGA 
               
               
                   
                   
                 CGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAAC 
               
               
                   
                   
                 GTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG 
               
               
                   
                   
                 AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAGACGCGGAT 
               
               
                   
                   
                 CCACAAACCTTTTGTTCGTCTTATACAAACCTTTTGTTCGTCTTATACAAACCTTTTGTTCGTC 
               
               
                   
                   
                 TTATACAAACCTTTTGTTCGTCTTATGTCGACCTCGAGAGATCTACGGGTGGCATCCCTGTGA 
               
               
                   
                   
                 CCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCT 
               
               
                   
                   
                 AATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGA 
               
               
                   
                   
                 GGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCT 
               
               
                   
                   
                 ATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGG 
               
               
                   
                   
                 GTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAG 
               
               
                   
                   
                 GCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTC 
               
               
                   
                   
                 CAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGT 
               
               
                   
                   
                 GAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 302 
                 HCC.V1.Cherry 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                   
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCCTAGGCTTCGAATCGATGAATTCGAAGCTTCTACCC 
               
               
                   
                   
                 ACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTCAAACTCGAAGTCGGCCATAT 
               
               
                   
                   
                 CCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCCGGACCCGGGGAATCCCCGTC 
               
               
                   
                   
                 CCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCATGCGCCATCGCCACGTCCTCGC 
               
               
                   
                   
                 CGTCTAAGTGGAGCTCGTCCCCCAGGCTGACATCGGTCGGGGGGGCCGTCGACAGTCTGCG 
               
               
                   
                   
                 CGTGTGTCCCGCGGGGAGAAAGGACAGGCGCGGAGCCGCCAGCCCCGCCTCTTCGGGGGCG 
               
               
                   
                   
                 TCGTCGTCCGGGAGATCGAGCAGGCCCTCGATGGTAGACCCGTAATTGTTTTTCGTACGCGC 
               
               
                   
                   
                 GCGGCTGTACGCGGAGGCCTGTTCGACCATCGCGTCGATGCCCGCGACGAGCAGGTCGAGG 
               
               
                   
                   
                 GCGAACTCGAAGTCCCGGTCCAGCATCTCCGCCACGGTGTCGCCGCCCCGGGCCGCCATGAT 
               
               
                   
                   
                 GTCCTGCGCGTCCTCGATGACGCCCGCGGTGTCCGGCACCTCGGTCACCGCGGTCATCGAGT 
               
               
                   
                   
                 CCTGGAAGTACTCCTCCGGACTCAGCCCGGTGTCCGCCACCCGGGCGAGGAAGCGGCCCTC 
               
               
                   
                   
                 GATGGTGCCGTAGCCGTAGACGAACTGGAAGACGGCCGAGATGGCGCCGGTCAGGCGGTGC 
               
               
                   
                   
                 GCGGGCAGCCCGCTGCGGCGCACGACGTTCTGCACCGCGCGGGAGAAGGCCAGCGAGTGCG 
               
               
                   
                   
                 GGCCGATGTTGAGGTAGGTGCCGACCAGCCGGGACGACCAGGGGTGGCGCACCAGCAGCG 
               
               
                   
                   
                 CCCGGTTCTCCCGGGCCAGGGCCCGCAGTTCCTCGCGCCAGTCGAGCCCGGCGTCCGGGTCC 
               
               
                   
                   
                 GGGTGGCGCAGCTCGCCGAAGACGGCGTCCAGGGCGAGCTCGAGCAACTGGTCCTTGGTGT 
               
               
                   
                   
                 CGACGTACCAGTACACGGACATCGCGGTGACGTTCAGCTCGGCGGCCAGGCGGCGCATCGA 
               
               
                   
                   
                 GAACCCCGTCAGGCCCTCCGTGTCCAGCAGCCGGACGGTGACCCCGGTGATCCGGTCCCGG 
               
               
                   
                   
                 TCGAGCCCGGACGGCTGCCCCCCACGGCGACCGCCGCGCCGCCCCTCCCCCGACAGCCACA 
               
               
                   
                   
                 CGCTGTCCCGCGGCCCCTCCCGCCCTGCCTTCGCCATGCGCACCTCTCCTCGACTCATACCGG 
               
               
                   
                   
                 TAGCGCTAGCGATGAGCTCTGGTAGTAGACTAGTGGCCCCCATTATATACCCTCTAGAGCAT 
               
               
                   
                   
                 ATGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGA 
               
               
                   
                   
                 GGGCTTTGTGTAGGGCGCGCCCCCGTAGCTTGGCGTAATCACATGTCCGTCGTTTTACAACG 
               
               
                   
                   
                 TCGTGACTGGGAAAACCCTGGCCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCC 
               
               
                   
                   
                 AGTCACGACGTTGTAAAACGACGGACATGTGAAATAGCGCTGTACAGCGTATGGGAATCTC 
               
               
                   
                   
                 TTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGGCGCGCCAGTTAATAATTAACTAGT 
               
               
                   
                   
                 TAATAATTAACTAGTTAATAATTAACTCATATGCTCTAGAGGGTATATAATGGGGGCCACTA 
               
               
                   
                   
                 GTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCGCCACCATGGTGAGCAAGGGCGAGGA 
               
               
                   
                   
                 GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTG 
               
               
                   
                   
                 AACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAG 
               
               
                   
                   
                 ACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCC 
               
               
                   
                   
                 TCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGA 
               
               
                   
                   
                 AGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGT 
               
               
                   
                   
                 GGTGACCGTGACCCAGGACTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTG 
               
               
                   
                   
                 CGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGG 
               
               
                   
                   
                 CCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGCGGCT 
               
               
                   
                   
                 GAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAA 
               
               
                   
                   
                 GCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAAC 
               
               
                   
                   
                 GAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCA 
               
               
                   
                   
                 TGGACGAGCTGTACAAGTAGGGTACCCAAACACCATTGTCACACTCCAAGATCTACGGGTG 
               
               
                   
                   
                 GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCA 
               
               
                   
                   
                 CCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATA 
               
               
                   
                   
                 TTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGG 
               
               
                   
                   
                 CCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATC 
               
               
                   
                   
                 TCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGC 
               
               
                   
                   
                 ATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGC 
               
               
                   
                   
                 CAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGG 
               
               
                   
                   
                 GATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 303 
                 HCC.V1. 
                 CAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAG 
               
               
                   
                 HSV-TK 
                 GTGTTGGGGAGGCAGTTACCGGGGCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGG 
               
               
                   
                   
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                   
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCCTAGGCTTCGAATCGATGAATTCGAAGCTTCTACCC 
               
               
                   
                   
                 ACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTCAAACTCGAAGTCGGCCATAT 
               
               
                   
                   
                 CCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCCGGACCCGGGGAATCCCCGTC 
               
               
                   
                   
                 CCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCATGCGCCATCGCCACGTCCTCGC 
               
               
                   
                   
                 CGTCTAAGTGGAGCTCGTCCCCCAGGCTGACATCGGTCGGGGGGGCCGTCGACAGTCTGCG 
               
               
                   
                   
                 CGTGTGTCCCGCGGGGAGAAAGGACAGGCGCGGAGCCGCCAGCCCCGCCTCTTCGGGGGCG 
               
               
                   
                   
                 TCGTCGTCCGGGAGATCGAGCAGGCCCTCGATGGTAGACCCGTAATTGTTTTTCGTACGCGC 
               
               
                   
                   
                 GCGGCTGTACGCGGAGGCCTGTTCGACCATCGCGTCGATGCCCGCGACGAGCAGGTCGAGG 
               
               
                   
                   
                 GCGAACTCGAAGTCCCGGTCCAGCATCTCCGCCACGGTGTCGCCGCCCCGGGCCGCCATGAT 
               
               
                   
                   
                 GTCCTGCGCGTCCTCGATGACGCCCGCGGTGTCCGGCACCTCGGTCACCGCGGTCATCGAGT 
               
               
                   
                   
                 CCTGGAAGTACTCCTCCGGACTCAGCCCGGTGTCCGCCACCCGGGCGAGGAAGCGGCCCTC 
               
               
                   
                   
                 GATGGTGCCGTAGCCGTAGACGAACTGGAAGACGGCCGAGATGGCGCCGGTCAGGCGGTGC 
               
               
                   
                   
                 GCGGGCAGCCCGCTGCGGCGCACGACGTTCTGCACCGCGCGGGAGAAGGCCAGCGAGTGCG 
               
               
                   
                   
                 GGCCGATGTTGAGGTAGGTGCCGACCAGCCGGGACGACCAGGGGTGGCGCACCAGCAGCG 
               
               
                   
                   
                 CCCGGTTCTCCCGGGCCAGGGCCCGCAGTTCCTCGCGCCAGTCGAGCCCGGCGTCCGGGTCC 
               
               
                   
                   
                 GGGTGGCGCAGCTCGCCGAAGACGGCGTCCAGGGCGAGCTCGAGCAACTGGTCCTTGGTGT 
               
               
                   
                   
                 CGACGTACCAGTACACGGACATCGCGGTGACGTTCAGCTCGGCGGCCAGGCGGCGCATCGA 
               
               
                   
                   
                 GAACCCCGTCAGGCCCTCCGTGTCCAGCAGCCGGACGGTGACCCCGGTGATCCGGTCCCGG 
               
               
                   
                   
                 TCGAGCCCGGACGGCTGCCCCCCACGGCGACCGCCGCGCCGCCCCTCCCCCGACAGCCACA 
               
               
                   
                   
                 CGCTGTCCCGCGGCCCCTCCCGCCCTGCCTTCGCCATGCGCACCTCTCCTCGACTCATACCGG 
               
               
                   
                   
                 TAGCGCTAGCGATGAGCTCTGGTAGTAGACTAGTGGCCCCCATTATATACCCTCTAGAGCAT 
               
               
                   
                   
                 ATGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGA 
               
               
                   
                   
                 GGGCTTTGTGTAGGGCGCGCCCCCGTAGCTTGGCGTAATCACATGTCCGTCGTTTTACAACG 
               
               
                   
                   
                 TCGTGACTGGGAAAACCCTGGCCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCC 
               
               
                   
                   
                 AGTCACGACGTTGTAAAACGACGGACATGTGAAATAGCGCTGTACAGCGTATGGGAATCTC 
               
               
                   
                   
                 TTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGGCGCGCCAGTTAATAATTAACTAGT 
               
               
                   
                   
                 TAATAATTAACTAGTTAATAATTAACTCATATGCTCTAGAGGGTATATAATGGGGGCCACTA 
               
               
                   
                   
                 GTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCCGCCACCATGGCTTCGTACCCCTGCCA 
               
               
                   
                   
                 TCAACACGCGTCTGCGTTCGACCAGGCTGCGCGTTCTCGCGGCCATAGCAACCGACGTACGG 
               
               
                   
                   
                 CGTTGCGCCCTCGCCGGCAGCAAGAAGCCACGGAAGTCCGCCTGGAGCAGAAAATGCCCAC 
               
               
                   
                   
                 GCTACTGCGGGTTTATATAGACGGTCCTCACGGGATGGGGAAAACCACCACCACGCAACTG 
               
               
                   
                   
                 CTGGTGGCCCTGGGTTCGCGCGACGATATCGTCTACGTACCCGAGCCGATGACTTACTGGCA 
               
               
                   
                   
                 GGTGCTGGGGGCTTCCGAGACAATCGCGAACATCTACACCACACAACACCGCCTCGACCAG 
               
               
                   
                   
                 GGTGAGATATCGGCCGGGGACGCGGCGGTGGTAATGACAAGCGCCCAGATAACAATGGGC 
               
               
                   
                   
                 ATGCCTTATGCCGTGACCGACGCCGTTCTGGCTCCTCATATCGGGGGGGAGGCTGGGAGCTC 
               
               
                   
                   
                 ACATGCCCCGCCCCCGGCCCTCACCCTCATCTTCGACCGCCATCCCATCGCCGCCCTCCTGTG 
               
               
                   
                   
                 CTACCCGGCCGCGCGATACCTTATGGGCAGCATGACCCCCCAGGCCGTGCTGGCGTTCGTGG 
               
               
                   
                   
                 CCCTCATCCCGCCGACCTTGCCCGGCACAAACATCGTGTTGGGGGCCCTTCCGGAGGACAGA 
               
               
                   
                   
                 CACATCGACCGCCTGGCCAAACGCCAGCGCCCCGGCGAGCGGCTTGACCTGGCTATGCTGG 
               
               
                   
                   
                 CCGCGATTCGCCGCGTTTACGGGCTGCTTGCCAATACGGTGCGGTATCTGCAGGGCGGCGGG 
               
               
                   
                   
                 TCGTGGCGGGAGGATTGGGGACAGCTTTCGGGGACGGCCGTGCCGCCCCAGGGTGCCGAGC 
               
               
                   
                   
                 CCCAGAGCAACGCGGGCCCACGACCCCATATCGGGGACACGTTATTTACCCTGTTTCGGGCC 
               
               
                   
                   
                 CCCGAGTTGCTGGCCCCCAACGGCGACCTGTACAACGTGTTTGCCTGGGCCTTGGACGTCTT 
               
               
                   
                   
                 GGCCAAACGCCTCCGTCCCATGCACGTCTTTATCCTGGATTACGACCAATCGCCCGCCGGCT 
               
               
                   
                   
                 GCCGGGACGCCCTGCTGCAACTTACCTCCGGGATGGTCCAGACCCACGTCACCACCCCCGGC 
               
               
                   
                   
                 TCCATACCGACGATCTGCGACCTGGCGCGCACGTTTGCCCGGGAGATGGGGGAGGCTAACT 
               
               
                   
                   
                 GAGGTACCCAAACACCATTGTCACACTCCAAGATCTACGGGTGGCATCCCTGTGACCCCTCC 
               
               
                   
                   
                 CCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAA 
               
               
                   
                   
                 ATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGG 
               
               
                   
                   
                 TGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGA 
               
               
                   
                   
                 ACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAG 
               
               
                   
                   
                 CGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGC 
               
               
                   
                   
                 TAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCC 
               
               
                   
                   
                 TAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCAC 
               
               
                   
                   
                 TGCTCCCTTCCCTGTCCTT 
               
               
                   
               
               
                 304 
                 HCC.V2. 
                 GGACCTGGATGCTGACGAAGGCTCGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG 
               
               
                   
                 HSV-TK 
                 CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC 
               
               
                   
                   
                 GAAAAGTGCCACCTGACGTCGGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTA 
               
               
                   
                   
                 TTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT 
               
               
                   
                   
                 TTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAA 
               
               
                   
                   
                 ATGTGGTATGGCTGATTATGATCCTCCTAGGTGAGGTAGTAGGTTGTATGGTTTGAGGTAGT 
               
               
                   
                   
                 AGGTTGTATGGTTTGAGGTAGTAGGTTGTATGGTTTGAGGTAGTAGGTTGTATGGTTATCGA 
               
               
                   
                   
                 TGAATTCGAAGCTTCTACCCACCGTACTCGTCAATTCCAAGGGCATCGGTAAACATCTGCTC 
               
               
                   
                   
                 AAACTCGAAGTCGGCCATATCCAGAGCGCCGTAGGGGGCGGAGTCGTGGGGGGTAAATCCC 
               
               
                   
                   
                 GGACCCGGGGAATCCCCGTCCCCCAACATGTCCAGATCGAAATCGTCTAGCGCGTCGGCAT 
               
               
                   
                   
                 GCGCCATCGCCACGTCCTCGCCGTCTAAGTGGAGCTCGTCCCCCAGGCTGACATCGGTCGGG 
               
               
                   
                   
                 GGGGCCGTCGACAGTCTGCGCGTGTGTCCCGCGGGGAGAAAGGACAGGCGCGGAGCCGCC 
               
               
                   
                   
                 AGCCCCGCCTCTTCGGGGGCGTCGTCGTCCGGGAGATCGAGCAGGCCCTCGATGGTAGACC 
               
               
                   
                   
                 CGTAATTGTTTTTCGTACGCGCGCGGCTGTACGCGGAGGCCTGTTCGACCATCGCGTCGATG 
               
               
                   
                   
                 CCCGCGACGAGCAGGTCGAGGGCGAACTCGAAGTCCCGGTCCAGCATCTCCGCCACGGTGT 
               
               
                   
                   
                 CGCCGCCCCGGGCCGCCATGATGTCCTGCGCGTCCTCGATGACGCCCGCGGTGTCCGGCACC 
               
               
                   
                   
                 TCGGTCACCGCGGTCATCGAGTCCTGGAAGTACTCCTCCGGACTCAGCCCGGTGTCCGCCAC 
               
               
                   
                   
                 CCGGGCGAGGAAGCGGCCCTCGATGGTGCCGTAGCCGTAGACGAACTGGAAGACGGCCGA 
               
               
                   
                   
                 GATGGCGCCGGTCAGGCGGTGCGCGGGCAGCCCGCTGCGGCGCACGACGTTCTGCACCGCG 
               
               
                   
                   
                 CGGGAGAAGGCCAGCGAGTGCGGGCCGATGTTGAGGTAGGTGCCGACCAGCCGGGACGAC 
               
               
                   
                   
                 CAGGGGTGGCGCACCAGCAGCGCCCGGTTCTCCCGGGCCAGGGCCCGCAGTTCCTCGCGCC 
               
               
                   
                   
                 AGTCGAGCCCGGCGTCCGGGTCCGGGTGGCGCAGCTCGCCGAAGACGGCGTCCAGGGCGAG 
               
               
                   
                   
                 CTCGAGCAACTGGTCCTTGGTGTCGACGTACCAGTACACGGACATCGCGGTGACGTTCAGCT 
               
               
                   
                   
                 CGGCGGCCAGGCGGCGCATCGAGAACCCCGTCAGGCCCTCCGTGTCCAGCAGCCGGACGGT 
               
               
                   
                   
                 GACCCCGGTGATCCGGTCCCGGTCGAGCCCGGACGGCTGCCCCCCACGGCGACCGCCGCGC 
               
               
                   
                   
                 CGCCCCTCCCCCGACAGCCACACGCTGTCCCGCGGCCCCTCCCGCCCTGCCTTCGCCATGCG 
               
               
                   
                   
                 CACCTCTCCTCGACTCATACCGGTAGCGCTAGCGATGAGCTCTGGTAGTAGACTAGTGGCCC 
               
               
                   
                   
                 CCATTATATACCCTCTAGAGCATATGTCTCACAAAGAGGGCTTTGTGTAGTCTCACAAAGAG 
               
               
                   
                   
                 GGCTTTGTGTAGTCTCACAAAGAGGGCTTTGTGTAGGGCGCGCCCCCGTAGCTTGGCGTAAT 
               
               
                   
                   
                 CACATGTCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCCTGCAAGGCGATTAAGT 
               
               
                   
                   
                 TGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGACATGTGAAATAGCG 
               
               
                   
                   
                 CTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGGCG 
               
               
                   
                   
                 CGCCAGTTAATAATTAACTAGTTAATAATTAACTAGTTAATAATTAACTCATATGCTCTAGA 
               
               
                   
                   
                 GGGTATATAATGGGGGCCACTAGTCTACTACCAGAGCTCATCGCTAGCGCTGGATCCCGCCA 
               
               
                   
                   
                 CCATGGCTTCGTACCCCTGCCATCAACACGCGTCTGCGTTCGACCAGGCTGCGCGTTCTCGC 
               
               
                   
                   
                 GGCCATAGCAACCGACGTACGGCGTTGCGCCCTCGCCGGCAGCAAGAAGCCACGGAAGTCC 
               
               
                   
                   
                 GCCTGGAGCAGAAAATGCCCACGCTACTGCGGGTTTATATAGACGGTCCTCACGGGATGGG 
               
               
                   
                   
                 GAAAACCACCACCACGCAACTGCTGGTGGCCCTGGGTTCGCGCGACGATATCGTCTACGTA 
               
               
                   
                   
                 CCCGAGCCGATGACTTACTGGCAGGTGCTGGGGGCTTCCGAGACAATCGCGAACATCTACA 
               
               
                   
                   
                 CCACACAACACCGCCTCGACCAGGGTGAGATATCGGCCGGGGACGCGGCGGTGGTAATGAC 
               
               
                   
                   
                 AAGCGCCCAGATAACAATGGGCATGCCTTATGCCGTGACCGACGCCGTTCTGGCTCCTCATA 
               
               
                   
                   
                 TCGGGGGGGAGGCTGGGAGCTCACATGCCCCGCCCCCGGCCCTCACCCTCATCTTCGACCGC 
               
               
                   
                   
                 CATCCCATCGCCGCCCTCCTGTGCTACCCGGCCGCGCGATACCTTATGGGCAGCATGACCCC 
               
               
                   
                   
                 CCAGGCCGTGCTGGCGTTCGTGGCCCTCATCCCGCCGACCTTGCCCGGCACAAACATCGTGT 
               
               
                   
                   
                 TGGGGGCCCTTCCGGAGGACAGACACATCGACCGCCTGGCCAAACGCCAGCGCCCCGGCGA 
               
               
                   
                   
                 GCGGCTTGACCT&#39;GGCTATGCTGGCCGCGATTCGCCGCGTTTACGGGCTGCTTGCCAATACGG 
               
               
                   
                   
                 TGCGGTATCTGCAGGGCGGCGGGTCGTGGCGGGAGGATTGGGGACAGCTTTCGGGGACGGC 
               
               
                   
                   
                 CGTGCCGCCCCAGGGTGCCGAGCCCCAGAGCAACGCGGGCCCACGACCCCATATCGGGGAC 
               
               
                   
                   
                 ACGTTATTTACCCTGTTTCGGGCCCCCGAGTTGCTGGCCCCCAACGGCGACCTGTACAACGT 
               
               
                   
                   
                 GTTTGCCTGGGCCTTGGACGTCTTGGCCAAACGCCTCCGTCCCATGCACGTCTTTATCCTGGA 
               
               
                   
                   
                 TTACGACCAATCGCCCGCCGGCTGCCGGGACGCCCTGCTGCAACTTACCTCCGGGATGGTCC 
               
               
                   
                   
                 AGACCCACGTCACCACCCCCGGCTCCATACCGACGATCTGCGACCTGGCGCGCACGTTTGCC 
               
               
                   
                   
                 CGGGAGATGGGGGAGGCTAACTGAGGTACCAACCATACAACCTACTACCTCAAACCATACA 
               
               
                   
                   
                 ACCTACTACCTCAAACCATACAACCTACTACCTCAAACCATACAACCTACTACCTCAAGATC 
               
               
                   
                   
                 TACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCC 
               
               
                   
                   
                 AGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTT 
               
               
                   
                   
                 CTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAAC 
               
               
                   
                   
                 CTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTC 
               
               
                   
                   
                 ACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGG 
               
               
                   
                   
                 ATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCA 
               
               
                   
                   
                 CCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCC 
               
               
                   
                   
                 AAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTT 
               
               
                   
               
            
           
         
       
     
     II. Other Compositions 
     In other aspects, the disclosure relates to compositions of vectors. In some embodiments, a vector comprises a contiguous polynucleic acid molecule described above. 
     In other aspects, the disclosure relates to compositions of engineered viral genomes. In some embodiments, the viral genome comprises a contiguous polynucleic acid molecule described above. In some embodiments, the viral genome is an adeno-associated virus (AAV) genome, a lentivirus genome, an adenovirus genome, a herpes simplex virus (HSV) genome, a Vaccinia virus genome, a poxvirus genome, a Newcastle Disease virus (NDV) genome, a Coxsackievirus genome, a rheovirus genome, a measles virus genome, a Vesicular Stomatitis virus (VSV) genome, a Parvovirus genome, a Seneca valley viral genome, a Maraba virus genome, or a common cold virus genome. 
     In other aspects, the disclosure relates to compositions of virions. As used herein, the term “virion” refers to an infective form of a virus that is outside of a host cell (e.g., comprising a DNA/RNA genome and a capsid protein). In some embodiments, a virion comprises the engineered viral genome described above. In some embodiments, the virion comprises a AAV-DJ capsid protein. In some embodiments, the virion comprises a AAV-B1 capsid protein, an AAV8 capsid protein, or an AAV6 capsid protein. 
     In other aspects, the disclosure relates to compositions comprising a contiguous polynucleic acid molecule described above, a vector described above, an engineered viral genome described above, or a virion described above. In some embodiments, the composition is a therapeutic composition further comprising a pharmaceutically-acceptable excipient or buffer. Exemplary pharmaceutical excipients and buffers are known to those having ordinary skill in the art. 
     III. Methods of Stimulating a Cell-Specific Event 
     In other aspects, the disclosure relates to methods of stimulating a cell-specific event in a population of cells. In some embodiments, the method of stimulating the cell-specific event comprises contacting a population of cells with a contiguous polynucleic acid molecule described above, a vector described above, an engineered viral genome described above, or a virion described above, wherein the cell-specific event is elicited via the level of output expressed in the cells of the population of cells. 
     In some embodiments, the population of cells comprises at least one target cell and at least one non-target cell. A target cell and a non-target cell type differ in levels of at least one endogenous transcription factor and/or the expression strength of at least one endogenous promoter or its fragment and/or at least one endogenous miRNA. In some embodiments, the expression levels of the output differs between target cells and non-target cells by at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 500, at least 1,000, or at least 10,000 fold. 
     In some embodiments, the method comprises contacting the population of cells with the contiguous polynucleic acid molecule or a composition comprising said contiguous polynucleic aid molecule, wherein: a) the population of cells comprises at least one target cell type and two or more non-target cell types, wherein the target cell type(s) and the non-target cell types differ in levels of one or more endogenous miRNAs (e.g., at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 20 endogenous miRNAs), such that the levels of the one or more endogenous miRNAs are at least two times higher (e.g., at least 2 times, at least 3 times, at least 4 times, at least 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, at least 10 times, at least 15 times, at least 20 times, at least 50 times, at least 100 times, at least 1000 times higher) in each of the two or more non-target cells relative to each of the target cells; and b) the contiguous polynucleic acid molecule comprises: (i) a first cassette encoding a RNA whose expression is operably linked to a transactivator response element, wherein the first RNA comprises: a nucleic acid sequence of an output; and one or more miRNA target sites corresponding to the one or more endogenous miRNAs; and (ii) a second cassette encoding a second RNA, wherein the second RNA comprises a nucleic acid sequence of a transactivator; wherein the transactivator of the second cassette, when expressed as a protein, binds and transactivates the transactivator response element of the first cassette. 
     In some embodiments, the method comprises contacting the population of cells with the contiguous polynucleic acid molecule or a composition comprising said contiguous polynucleic aid molecule, wherein: a) the population of cells comprises at least one target cell type and two or more non-target cell types, wherein the target cell type(s) and the non-target cell types differ in levels of one or more endogenous miRNAs (e.g., at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 20 endogenous miRNAs), such that the levels of the one or more endogenous miRNAs are at least two times higher (e.g., at least 2 times, at least 3 times, at least 4 times, at least 5 times, at least 6 times, at least 7 times, at least 8 times, at least 9 times, at least 10 times, at least 15 times, at least 20 times, at least 50 times, at least 100 times, at least 1000 times higher) in each of the two or more non-target cells relative to each of the target cells; and b) the contiguous polynucleic acid molecule comprises cassette encoding a mRNA whose expression is operably linked to a transactivator response element, wherein the RNA comprises: a nucleic acid sequence of an output; a nucleic acid sequence of a transactivator; and one or more miRNA target sites corresponding to the one or more endogenous miRNAs; and wherein the transactivator, when expressed as a protein, binds and transactivates the transactivator response element of the cassette. 
     In some embodiments, the target cell type(s) and the non-target cell types differ in levels of one or more endogenous transcription factors (e.g., at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 20 endogenous transcription factors), wherein the contiguous nucleic acid molecule further comprises one or more transcription factor response element corresponding to the endogenous transcription factor(s). 
     In some embodiments, the contacting with the host cell with a contiguous polynucleic acid molecule described above or a vector described above occurs via a non-viral delivery method. Examples include, but are not limited to, transfection (e.g., DEAE dextran-mediated transfection, CaPO 4 -mediated transfection, lipid-mediated uptake, PEI-mediated uptake, and laser transfection), transformation (e.g., calcium chloride, electroporation, and heat-shock), gene transfer, and particle bombardment. 
     In some embodiments, the population of cells is contacted ex vivo (i.e., a population of cells is isolated from an organism, and the population of cells is contacted outside of the organism). In some embodiments, the population of cells is contacted in vivo. 
     As used herein, the term “endogenous”—in the context of a cell—refers to a factor (e.g., protein or RNA) that is found in the cell in its natural state. In some embodiments, an endogenous transcription factor may bind and activate a promoter element of a regulatory component of at least one cassette (e.g., a transcription factor response element). In some embodiments, an endogenous miRNA may complement a miRNA target site of a regulatory component or response component of at least one cassette. 
     In some embodiments, a “transactivator” and corresponding “transactivator response element” will be selected such that the transactivator will specifically bind to the “transactivator response element” but bind as little as possible to response elements naturally present in the cell. In some embodiments, the DNA binding domain of a transactivator protein will not efficiently bind native regulatory sequences present in the cell and, therefore, will not trigger excessive side effects. 
     In some embodiments a target cell and a non-target cell are different cell types. 
     In some embodiments, a target cell is a cancerous cell and a non-target cell is a non-cancerous cell. In some embodiments, a target cell may be a cancerous hepatocellular carcinoma cell or a cholangiocarcinoma cell and a non-target cell may be a parenchymal and non-parenchymal liver cells, including hepatocytes, phagocytic Kupffer cells, stellate cells, sinusoidal endothelial cells. 
     In some embodiments, a target cell is a hepatocyte and a non-target cell is a non-hepatocyte (e.g., a myocyte). In other embodiments, a target cell and a non-target cell are the same cell-type (e.g., both are hepatocytes), but nonetheless, differ in levels of at least one endogenous transcription factor and/or at least one endogenous miRNA. For example, a target cell may be a senescent muscle cell and a non-target cell may be a non-senescent muscle cell. 
     In some embodiments, the target cells are tumor cells and the cell-specific event is cell death. In some embodiments, the target cells are senescent cells and the cell-specific event is cell death. In some embodiments, the cell death is mediated by immune targeting through the expression of activating receptor ligands, specific antigens, stimulating cytokines, or any combination thereof. In some embodiments, the method further comprises contacting the population of cells with a prodrug or a non-toxic precursor compound that is metabolized by the output into a therapeutic or a toxic compound. 
     In some embodiments, the target cells differentially express a factor relative to wild-type cells (e.g., healthy and/or non-diseased) of the same type and the cell-specific event is modulating expression levels of the factor. 
     In some embodiments, output expression ensures the survival of the target cell population while the non-target cells are eliminated due to lack of output expression and in the presence of a cell death-inducing agent. In other embodiments, the output ensures the survival of the non-target cell population while the target cells are eliminated due to output expression and in the presence of a cell death-inducing agent. 
     In some embodiments, the target cells comprise a particular phenotype of interest such that output expression is limited to the cells of this particular phenotype. 
     In some embodiments, the target cells are a cell type of choice and the cell-specific event is the encoding of a novel function, through the expression of a gene naturally absent or inactive in the cell type of choice. 
     In some embodiments, the population of cells comprises a multicellular organism. In some embodiments, the multicellular organism is an animal. In some embodiments, the animal is a human. 
     IV. Methods of Diagnosing and/or Treating a Disease or a Condition 
     In some aspects, the disclosure relates to methods of diagnosing a disease or a condition (e.g., cancer) in a subject exhibiting one or more signs or symptoms of the disease or condition. As used herein, the term “diagnose” refers to a process of identifying or determining the nature and/or cause of a disease or condition. In some embodiments, the method comprises administering a contiguous polynucleic acid molecule described above, a vector described above, an engineered viral genome described above, or a virion described above to a subject exhibiting one or more signs or symptoms associated with a disease or condition, wherein the levels of the output indicates the presence or absence of the disease or condition. 
     In some aspects, the disclosure relates to methods of treating a disease or condition (e.g., cancer). As used herein, the term “treat” refers to the act of preventing the worsening of one or more symptoms associated with a disease or condition and/or the act of mitigating one or more symptom associated with a disease or condition. In some embodiments, the method comprises administering a contiguous polynucleic acid molecule described above, a vector described above, an engineered viral genome described above, or a virion described above to a subject having the disease or condition. 
     In some embodiments related to treating the disease or condition, the method of administration comprises an intravenous delivery of the vectors described above. In some embodiments, the method of administration comprises more than one act of intravenous delivery of the vectors described above. In some embodiments, the method of administration comprises an intratumoral delivery of the vectors described above, in one or more dosing. In some embodiments, the method of administration comprises a transarterial delivery of the vectors described above, in one or more dosing. In some embodiments, the method of administration comprises an intramuscular delivery, an intranasal delivery, subretinal delivery, or oral delivery, 
     In some embodiments, the method of treating the disease further comprises the administration of a pro-drug in one or more dosings. In some embodiments, the delivery off the prodrug is intravenous, transarterial, or intraperitoneal. In some embodiments, the prodrug is ganciclovir. 
     In some embodiments, the method of treating the disease further comprises the administration of another therapy such as a small molecule, a biologic, a monoclonal antibody, another gene therapy product, or a cell-based therapeutic product. 
     In some embodiments, the diseases or condition is cancer. Exemplary cancers that can be treated by the methods described herein include, but are not limited to, .hepatocellular carcinoma (HCC), metastatic colorectal cancer (mCRC), any other cancer metastasized to the liver, lung cancer, breast cancer, retinoblastoma, and glioblastoma. 
     Exemplary cancers that can be treated by the methods described herein include, but are not limited to, hepatocellular carcinoma (HCC), metastatic colorectal cancer (mCRC), lung cancer, breast cancer, retinoblastoma, glioblastoma. 
     In some embodiments, the cancer is hepatocellular carcinoma (HCC)). Indeed, therapeutic options for HCC are limited (Llovet and Lencioni, 2020), creating an urgent need to explore novel modalities for breakthroughs. The methods described herein significantly advance current HCC treatment methodologies. 
     EXAMPLES 
     Example 1. Multiplex Diagnostic Circuits Translate to Gene Therapy Vectors 
     Experiments were designed to assess whether logic gates put together from multiple disjointed components (i.e., one gene per plasmid and characterized in transient transfection of cell lines) could be re-engineered to fit into a therapeutically relevant vector and studied as a therapeutic candidate in an animal disease model. It was previously shown that integration of sensors for transcription factors (TF) SOX9/10 and HNF1A/B by a multi-plasmid system implementing an AND logic between these sensor&#39;s activity elicited a strong response when transiently transfected into HuH-7 cells (Angelici et al., 2016). SOX9 is a prognostic marker associated with advanced HCC (Richtig et al., 2017). Interestingly, the SOX9 response element is likely to be bound by SOX4, another TF whose overexpression is associated with a malignant HCC phenotype (Liao et al., 2008; Uhlen et al., 2017). HNF1A and HNF1B are known liver housekeeping factors (Harries et al., 2009); although, they are also expressed in other organs of the GI tract. 
     Experiments were designed to gauge whether the previously described multi-plasmid system could be adapted to a contiguous DNA cassette and eventually packaged in a viral vector. To this end, circuit components shown to implement the logic “SOX9/10 AND HNF1A/B” in a multi-plasmid setting (Angelici et al., 2016), comprising a SOX9/10-driven PIT-based activator (PIT::RelA or PIT::VP16) (Fussenegger et al., 2000), as well as a fluorescent output protein synergistically driven by PIT and HNF1A/B, were cloned between ITRs in an adeno-associated viral (AAV) transfer vector either in a divergent or convergent orientation ( FIG.  1 A ). The resulting plasmids were transiently transfected into HEK293 cells, and the TF inputs SOX10 and HNF1A were expressed ectopically from TRE-driven plasmids to generate all four logical input combinations to this gate. Interestingly, while the trend was preserved in all four cases, the different variants differ markedly in their absolute ON levels when both inputs are present ( FIG.  1 B ). The same constructs were also transfected into HuH-7 and HeLa cells, where the endogenous expression of SOX9/10 and HNF1A/B is expected to induce the circuit in the former and not activate it in the latter. In this case, the differences were less pronounced, yet the divergent orientation generated somewhat higher output. 
     The AND gate strategy is a way to activate the output in the desired cell type, and the augmentation of this activation designed by incorporation of intentional “Off” switches, equivalent to NOT gates, which would comprise additional safety layer in the context of a therapy. To this end, microRNA targets were incorporated in the 3′-UTR of the output gene, as well as in the 3′-UTR of the PIT-derived component. The choice of specific inputs, including miR-424, miR-126 and miR-122, was made on the basis of previously-performed profiling (Dastor et al., 2018). The miR-424 target was initially introduced, and the four resulting constructs ( FIG.  1 D ) were again tested for their response to ectopic TF combinations in HEK cells ( FIG.  1 E ) and in the presence of endogenous inputs in HuH-7 and HeLa cells ( FIG.  1 F ). Marked and consistent differences were observed in performance. The convergent constructs failed to respond to the ectopic inputs in HEK cells and responded with greatly reduced intensity in HuH-7 cells, compared to the divergent ones. This fact highlights the complexity of the transition from circuits carried on disparate plasmids and circuits integrated on a contiguous backbone compatible with a gene therapy delivery vector. Next, the two divergent cassettes underwent more extensive logic characterization including both the TF and the miR-424 mimic input. Both constructs responded as expected, implementing the logic “SOX10 AND HNF1A AND NOT(miR-424)” ( FIG.  1 G ). To confirm that high miR-424 expression also overrides output activation with endogenous TF inputs, miR-424 mimic was transfected into HuH-7 cells and was found to turn off output expression to an almost background level ( FIG.  1 H ). Next, the miR-424 targets were replaced with miR-126 targets. The new set of constructs was tested only in HuH-7 cells with respect to its response to exogenous miR-126, and the results were similar to miR-424 and consistent with expectation ( FIG.  1 I ). To conclude this design stage, the divergent constructs without miRNA targets, with miR-424 or miR-126 targets were evaluated for their capacity to distinguish HCC cell lines HuH-7 and HepG2 from HeLa cells ( FIG.  1 J ). 
     The next step is the incorporation of the cassettes into viral vectors and their evaluation with respect to logic performance prior to preclinical translation. It is known that AAV-delivered genomes form concatemers in human cells (Duan et al., 2003), and this would comprise additional layer of complexity compared to the DNA cassette encoding the AAV genome but not packaged and delivered with the help of an AAV capsid. To this end, ITR-flanked genomes were used, and small quantities of DJ-pseudotyped (Grimm et al., 2008) AAV vectors were manufactured. The vectors were used to transduce two HCC cell lines, HepG2 and HuH-7, and two non-HCC cell lines, HeLa and HCT-116. The results showed high expression in the target cells and very low expression in non-target cells (FIG.  1 K). Some additional effects are apparent, for example the reduction of the output expression obtained with a vector bearing a T424 targets in HuH-7 cells, compared to the vector without miRNA targets, which is much stronger than the reduction observed with naked DNA cassettes. 
     In order to get preliminary information which of the two miRNA targets (T424 or T126) would fare better in vivo, experiments were designed to assess which of them would perform a key protecting function (i.e., enable discrimination between HCC cells and healthy hepatocytes). Primary mouse hepatocytes were isolated for in vitro culture. The primary hepatocytes and the HCC cell were transduced with AAV-DJ packaged genetic reporters (Dastor et al., 2018) for miR-424, miR-126 as well as miR-122, a known liver miRNA that was shown to turn off gene expression efficiently in the liver in vivo (Dastor et al., 2018; Della Peruta et al., 2015) and that is known to be downregulated in a subset of HCC tumors (Coulouarn et al., 2009). The results of this testing ( FIG.  1 L ) show that surprisingly, high expression counts of miR-424 and miR-126 in the liver did not translate to high biological knock-down activity in hepatocytes. Only miR-122 was consistently active. miR-122 was inactive in HepG2 cell line, but it showed partial activity in HuH-7 cell line, suggesting that the inclusion of this miRNA target would be beneficial for a subset of HCC tumors but not for all of them. Despite this fact, the circuit was further investigated with miR-122 for its specificity and antitumor potential in a pilot experiment setting. The impact of different miRNA target arrangements was also tested to assess how their number affects the overall output suppression in the presence of the miRNA input. Four different cassettes were tested, and it was found that increasing the number of targets, and placing the targets both in the output and in the PIT 3′-UTR, increases the repression ( FIGS.  1 M- 1 N ). This provides another knob that can be used in two ways: to increase the knockdown of the output in not-target cells, but also decrease the knockdown in target cells that express partial level of the miRNA input. 
     Example 2. Initial Evaluation of the First HCC-Targeting Circuit Variant in the Translational Context 
     Based on the reporter investigation, a circuit variant was constructed bearing miR-122 targets. The PIT::VP16 activator variant was used due to its lower DNA payload and increased available footprint for the output gene. The circuit with mCherry output, dubbed HCC.V1-mCherry, was packaged into DJ-pseudotyped AAV vectors and re-tested in its ability to discriminate HCC cell lines from primary murine hepatocytes. The data highlight that the full circuit generates highly specific expression in HepG2 and Hep3B cell lines compared to primary hepatocytes, while in HuH-7 the circuit generates reduced output due to intermediate activity of miR-122 in these cell lines ( FIG.  2 A ). Accordingly, this tumor-targeting program was evaluated in a pilot experiment in the context of orthotopic xenograft tumor model employing HepG2 cells in NSG mice. For the purpose of tumor establishment and tracking, HepG2 cells were stably modified with a lentiviral vector encoding an mCitrine fluorescent protein and firefly luciferase gene, and sorted for homogenous mCitrine expression. The tumors were established by splenic injection of 1M HepG2-LC cells and subsequent spleen dissection. 
     Prior to in vivo experiments, in vitro efficacy tests were performed comparing primary hepatocytes, HepG2 cells and HeLa cells as another negative control cell line. The vector, bearing HSV-TK output gene and dubbed AAV-DJ-HCC.V1-HSV-TK, requires GCV as a prodrug to elicit cytotoxicity with marked bystander effect (Freeman et al., 1993). The data ( FIG.  2 B ) showed that indeed, HepG2 cells were selectively eliminated by the circuit as well as the control constitutive vector, while primary hepatocytes and HeLa cells were eliminated by the constitutive vector but were not affected by the circuit-bearing vector. Notably, the circuit eliminated HepG2 cells better than the constitutive control, highlighting the importance of high output expression driven by the tailored TF logic, compared to non-tailored constitutive vector. 
     To gauge antitumor efficacy in vivo, AAV-DJ-HCC.V1-HSV-TK was delivered to HepG2 tumor bearing mice in two consecutive injections, three days apart. The four experimental groups (n=2 in this pilot) included the AAV-DJ-HCC.V1-HSV-TK in combination with GCV regimen (treatment arm), the same vector alone without GCV, sham injection supplemented with GCV regimen, and a sham PBS injection and no GCV. Live imaging of tumor progression in the treated animals ( FIG.  2 C ), and post-mortem analysis of the total tumor load in the liver with bioluminescence ( FIGS.  2 D- 2 E ), clearly demonstrated that the gene therapy vector bearing the full circuit program in combination with the HSV-TK output and GCV regimen has strong antitumor activity, which is absent in any of the control arms. A low tumor load in one of the animals in the PBS control arm resulted from the initial poor tumor implantation ( FIG.  2 F ), and in general all three control arms behaved the same, resulting in final tumor load proportional to the initial load, meaning that the tumor growth was governed by the same dynamics. The animals in the treatment arm of the pilot are obvious outliers, providing another evidence that the treatment was efficacious in reducing tumor load. 
     Example 3. Engineering of a Tumor-Targeting Program with Higher Specificity and Broader Scope 
     Encouraged by the outcome of the pilot experiment, it was sought to modify the tumor targeting program and in parallel to perform a more thorough evaluation of the circuit mechanism of action in vitro and in vivo. It was hypothesized that the combination of SOX9/10 and HNF1A/B inputs is a good starting point to restrict the expression to liver and liver tumors, however, previous data on miR-122 activity in vivo showed that its activity was restricted to liver (Dastor et al., 2018) and therefore one would have to rely on the TF-only component of the circuit for all other organs, which might become a problem if a vector capsid with broad organ specificity would be used. In addition, while miR-122 is a good classification marker to separate healthy hepatocytes from some HCC subtypes, it is not a universal HCC feature. Accordingly, the search was focused on miRNA inputs that might enable broader classification capacity of liver vs liver tumors, as well as protect additional organs. The point of origin for this search was 1) a miRNA profiling dataset obtained previously (Dastor et al., 2018) and 2) an extensive literature analysis for highly-expressed microRNAs in different organs. HuH-7 cells and healthy hepatocytes were profiled in the earlier experiments, and attempts were first made to identify a miRNA highly expressed in the hepatocytes but downregulated in HuH-7 cells ( FIG.  3 A ). The miRNA set selected based on the count ratio in the NGS profiling dataset, included miR-122 (as a reference), miR-424, miR-126-5p, miR-22, miR-26b and let-7c. Bidirectional miRNA reporters (Dastor et al., 2018) were constructed and packaged into AAV-DJ vectors, to ensure high delivery efficiency to primary hepatocytes in vitro ( FIG.  3 B ). Biological activity of the miRNA candidates was measured in HuH-7, HepG2, and primary isolated murine hepatocytes. Of the tested miRNAs, let-7c showed the highest differential activity; moreover, it was downregulated in both HuH-7 and HepG2 cells ( FIG.  3 C ). Interestingly, retrospective analysis ( FIG.  3 D ) comparing the NGS counts with the biological activity shows only a very superficial correlation, highlighting the importance of functional testing of candidate inputs. 
     Literature search and the examination of the profiling dataset for potential organ-protecting miRNA resulted in a set of miRNAs: miR-424 (kidney and other organs), miR-208a and miR-208 (heart), miR-216A, miR-217, and miR-375 (pancreas). Let-7c, a candidate for liver protection found based on the in vitro screening campaign, was added to this list. For each of these miRNAs, a bidirectional reporter was engineered and packaged in a B1-pseudotyped AAV vector (Choudhury et al., 2016), chosen due to its broad biodistribution. A control vector was made bearing a presumably neutral miRNA target (“TFF5”). (However, as the data revealed, this target was responding to miRNA inputs in at least some organs.) The vectors were injected systemically into healthy mice, and reporter expression was evaluated 3 weeks post-injection in the various organs. Strong biodistribution was found in liver, pancreas, heart and kidney, and the analysis was focused on these organs. Let-7c was the only miRNA from the set that showed potential as a healthy liver-specific input in vivo. In the pancreas in vivo, both miR-217 and miR-375 showed activity as expected from literature data; however, let-7c had the strongest response. In the heart, miR-208a and miR-208b showed activity consistent with prior data, yet again let-7c had the strongest response. Lastly, miR-424 was active in the kidney as expected, however, in this organ as well let-7c gave the strongest effect ( FIG.  3 EF ). 
     In summary, the combination of in vitro and in vivo data showed that for the purpose of this study, let-7c could serve as a “universal” input, playing a role of a protective miRNA input for multiple organs at once and at the same time, being strongly downregulated in both HCC cell lines used in the tumor study. Accordingly, the next iteration of the circuit, dubbed HCC.V2, implements the program “SOX9/10 AND HNF1A/B AND NOT(let-7c)”. 
     Example 4. Mechanism of Action In Vitro and In Vivo 
     Using AAV-DJ capsid as an efficient vehicle for cell transduction in vitro, and AAV-B1 as a capsid with broad biodistribution in vivo, an extensive mechanistic study of the AAV-packaged circuit was performed. Earlier in the study, the logic programs were analyzed and validated by transfecting circuit-carrying plasmid DNA into a background cell line that does not express any of the inputs; and then by systematic ectopic expression of all possible input combinations, comparing the results to the expectation. In the case of a viral vector, this strategy is now longer valid, because it is next to impossible to co-deliver individual ectopic inputs when the circuit itself is delivered via AAV transduction. Indeed, the more interesting question is how the vector responds to endogenously expressed inputs, because the cell classification in the context of a therapy has to rely on, and adequately respond to, endogenous inputs. A proof of mechanism thus comprises the question whether the output of the full circuit in a cell type is consistent with the activity of individual circuit inputs in these cells and the logic program of the circuit. 
     Accordingly, individual genetic sensors were created and packaged into AAV-DJ for every circuit input (AAV-DJ.C.SOX-FB.mCherry and AAV-DJ.C.HNF1-FB.mCherry for SOX9/10 and HNF1A/B feedback-amplified sensors, respectively); let-7c sensor (AAV-DJ.C.let-7c.mCherry); a partial circuit implementing AND gate only (AAV-DJ.C.TF-AND.mCherry); a full circuit (AAV-DJ.HCC.V2.mCherry); and a constitutive reporter serving as a reference (AAV-DJ.C.CMV.mCherry) ( FIG.  4 A ). The outputs of these constructs were measured in 10 cell lines and primary hepatocytes. The results ( FIGS.  4 B- 4 C ) show that the response of the multi input circuit is consistent with the expression of the individual inputs, confirming that the mechanism of action is preserved between the plasmid-based and viral vector-packaged system. Strong response of both individual sensors for SOX9/10 and HNF1A/B is needed to trigger high response of the TF-AND gate; and strong response of the TF-AND gate and the lack of response of the let-7c sensor is required to achieve high output of the complete program. 
     For in vivo characterization, B1-pseudotyped vectors packaging, respectively, a constitutive control AAV-B1.C.CMV.mCherry, a TF-only AND gate AAV-B1.C.TF-AND.mCherry, a let-7c reporter AAV-B1.C.let-7c.mCherry, and a full circuit AAV-B1.HCC.V2.mCherry, and expressing mCherry as the output, were systemically injected into mouse tail vein and the mCherry expression was evaluated 3 weeks post-injection in various organs. The expression was quantified in fresh organ slices by image processing. The results ( FIGS.  5 A- 5 B ) highlight the complex synergistic action of the multiple inputs and their diverse role in different organs. In the liver, the AND-gate resulted in the reduction of the number of positive cells compared to the constitutive control, but in elevated expression on cells that exhibited positive expression. The let-7c reporter showed reduced expression compared to control, but the residual expression was clearly above background. The complete circuit resulted in expression virtually indistinguishable from background. In the pancreas, the AND gate-controlled expression and let-7c controlled expression resulted in large reduction in output expression, yet in each case the expression was above background. As in the liver, the complete targeting program did not generate any detectable expression above background. In the heart, either the AND gate or the let-7c rendered background-level expression on their own, and when combined in a complete circuit. In the kidney the situation is similar to pancreas, in that neither AND gate nor let-7c regulation bring down the expression to background, while the complete program does. In summary, the dataset strongly supports the hypothesis that a multi-input logic circuit is required to achieve highly efficient de-targeting from healthy organs in vivo; the synergistic effect of multiple inputs, as abstracted by the logic program “SOX9/10 AND HNF1A/B and NOT(let-7c)” is apparent in three out of four cases. Experiments were then designed to determine if the same program is able to efficiently target tumors in vivo, and injected a B1-typed AAV-B1.HCC.V2.mCherry circuit with mCherry output to tumor-bearing NSG mice. The data ( FIG.  5 C ) show that indeed, the tumor is targeted specifically and efficiently in vivo while other organs do not express the output, consistent with data in  FIGS.  5 A- 5 B . 
     Example 5. Antitumor Efficacy In Vitro and In Vivo 
     As the circuit program showed excellent tumor-specific expression and de-targeting from major organs in vivo, detailed evaluation of its antitumor activity was performed using HSV-TK enzyme in combination with the prodrug ganciclovir as a benchmark antitumor actuator. The circuit was dubbed HCC.V2-HSV-TK. The testing was done along the lines similar to the pilot experiment ( FIG.  2   ) but with larger animal groups and extended number of experimental arms. DJ-pseudotyped vectors, including a constitutive control and a complete circuit were manufactured and their dose-response to ganciclovir evaluated in HuH-7, HepG2, and HeLa cell lines and in primary hepatocytes cultured in vitro. As expected, Huh-7 and HepG2 cells were targeted equally by the constitutive vector and the circuit AAV-DJ.HCC.V2-HSV-TK, while both HeLa negative control cells and primary hepatocytes were sensitive to the constitutive vectors but were not eliminated by the fully furnished circuit ( FIG.  6 A ). In addition, AAV-DJ.HCC.V2-HSV-TK is more potent than AAV-DJ.HCC.V1-HSV-TK in HuH-7 cells, due to the use of let-7c sensor which is not downregulated in these cells. However, AAV-DJ.HCC.V1-HSV-TK was still active in HuH-7 cells due to incomplete shut-down by miR-122 ( FIG.  6 B ). 
     Next, DJ-pseudotyped AAV vectors harboring the circuit were delivered systemically to HepG2-LC tumor-bearing mice ( FIG.  7 A ). The experimental arms without ganciclovir included the sham injection (saline); the vector AAV-DJ.C.TF-AND-HSV-TK encoding the TF-AND program; and the vector encoding the full circuit AAV-DJ.HCC.V2-HSV-TK. The arms with ganciclovir mirrored the arms above with respect to tail vein delivery of a vector or a sham, followed by a regimen of ganciclovir injections; namely: included sham injection+GCV; AND-gate circuit+GCV; and a complete circuit+GCV. The animals (n=4 per arm) were followed for their tumor load using in vivo bioluminescence, and for their well-being using score sheet criteria. The data ( FIGS.  7 B- 7 F ) indicate that mice treated with the vector harboring the full HCC.V2-HSV-TK program furnished with HSV-TK output and supplemented with GCV regimen, show robust and reproducible containment and then regression of their tumor load, while the control groups without GCV, or the group that was only injected with GCV, show exponential tumor load increase over time. The vector encoding the AND gate with HSV-TK output, AAV-DJ-C.TF-AND-HSV-TK, exhibited similar antitumor effect compared to AAV-DJ.HCC.V2-HSV-TK, yet also triggered strong adverse effects, and therefore the animals in this arm had to be euthanized prior to scheduled completion. The arm treated with the complete AAV-DJ.HCC.V2-HSV-TK circuit, on the other hand, showed extended reduction in tumor load without obvious adverse effects. These results unequivocally illustrate the tight link between the targeting specificity in vivo ( FIGS.  5 A- 5 D ) and the magnitude of adverse effects in vivo. Accordingly, in the future the presence of output expression outside of the tumor as gauged from a fluorescent output expression, will constitute a pre-screening stage that need not be evaluated for their toxicity with functional outputs. 
     Example 6. In Vivo Comparison of AAV-B1 and AAV-DJ Pseudotypes Circuit Driven HCC Targeting 
     Given the broad tropism and strong in vivo transduction observed for the B1-typed AAV capsid and the extensive multi-organ detargeting accomplished placing gene expression under the control of the HCC.V2 program, it was reasoned that the resulting B1-typed AAV-B1.HCC.V2 circuit might yield high tumor transduction without compromising selectivity. To investigate this possibility, circuit output (mCherry) was compared when the AAV-B1.HCC.V2-mCherry full circuit output is delivered using a B1 capsid in place of the DJ capsid used in previous efficacy studies. The data ( FIG.  8 A ) show that, when administered at the same dosage, the B1 typed circuit vastly outperforms the tumor expression levels of all DJ variants (AAV-DJ.HCC.V2.mCherry, TF-only AND gate AAV-DJ.C.TF-AND.mCherry or AAV-DJ.C.CMV.mCherry) while keeping its selectivity towards neighboring liver tissue. The intratumoral output expression was about 40 times higher ( FIG.  8 B ) and resulted in intense fluorescence even in the core section of large tumor nodules. The strong selective expression combined with tumor penetration suggest circuit targeting, coupled to B1-typed capsid as promising candidates for HCC gene therapies. 
     Example 7. Combination of miR-let-7c and miR-122 
     In vitro efficacy data show that while HCC.V1 fully protects hepatocytes even at high dosage ( FIG.  2 B ), the same program shows only a partial reduction in HUH-7 cell killing efficiency when compared to HCC.V2 ( FIG.  5 B ) and results in almost comparable performance for high viral dosage. This difference is in agreement with the tighter gene repression observed in Hepatocytes compared to HUH-7 cells ( FIG.  2 A ). 
     As established herein, changes in the number and arrangement of miR-122 targets can be used to modulate the repression strength resulting in different expression levels in cell lines with different miR-122 levels ( FIG.  1 M ). It was hypothesized that a reduction in miR-122 repression efficiency through changes in target number, arrangement, or via the use of imperfectly complementary targets could be used to increase circuit efficacy in HUH-7 (even at lower viral dosage), at the risk of a partial reduction of liver detargeting. 
     From these data, a HCC.V3 circuit that combines the miR-Let7c targets from HCC.V2 with weaker miR-122 repression ( FIG.  9 A ) is expected to outperform both the HCC.V3 circuit and the HCC.V2 circuit. The repression strength elicited by miR-122 can be tuned by changing the number and positioning of T-122 targets, by introducing imperfectly complementary targets or by a combination of the two approaches. Imperfectly complementary target can be obtained by introducing random mutations in the sequence flanking the miRNA seed sequence or by using miR-122 targets derived from conserved 3′ UTR of genes regulated by the miRNA ( FIG.  9 B ). The candidate that maximize the desired combination of liver protection and efficacy against HCC cells (HUH-7 in particular) can be selected. 
     It is expected that HCC.V3 will exhibit generalized miRNA detargeting from major organs (Let-7c) and benefit from combined protection (Let7c and miR-122) in the liver without significant reductions in its efficacy both in HepG2 and HUH-7. Being the organ with the highest biodistribution for most viral vectors, achieving the tightest possible liver detargeting is particularly desirable and might lead to further increases in the therapeutic window. 
     Example 8. Discussion 
     This disclosure shows a path to the clinical translation of logic gene circuit approaches. Three underlying pillars are necessary to support such a translation, namely: (1) the knowledge of the molecular make up of a disease; (2) the availability of a platform that enables taking advantage of this knowledge; and (3) the translatability of this platform to a clinically-relevant therapeutic modality come together to deliver a viable therapeutic candidate with promising in vitro and in vivo efficacy and safety profile. The extensive mechanistic characterization described herein highlights the unique properties of multi-input cell classifiers, constructed in rational bottom-up fashion following a systematic procedure, compared to its individual components. Importantly, it is demonstrated herein that targeting specificity as gauged by reporter outputs tightly correlates with both efficacy and adverse effects in vivo. 
     Specific expression and other modalities of therapeutic control, such as timing and dosage, are the next frontier of gene therapy not only for cancer but also for other indications. A large effort has been invested into the development of novel capsids with preferential tissue targeting, as well as promoter elements for specific tissue expression. Notably, both lines of work rely on extensive screening of large libraries and they do not guarantee success; moreover, the claim of specificity can only be made in the presence of large panel of counter samples. For human therapy, these samples must be of human origin. Due to the large diversity of human tissues, superimposed on the large library sizes for capsid and/or promoter screen, will make this effort prohibitively complex. The bottom-up approach described herein uses rational design to create combinatorial specificity from multiple individual inputs. Narrowing down the candidate input space by profiling puts the engineering of complex programs able to address heterogeneous cell populations (as in our example of Huh-7 and HepG2 cells) on a rational, forward design background. This approach does not exclude the use of targeted capsids or specific promoters: they can be applied as needed. However, for a disseminated disease such as cancer, broad tropism capsid may be preferential; the burden of specific expression is then shifted to the classified program encoded in the genetic payload of the therapy. In other cases, capsid specificity and the classifier program can be used synergistically to achieve the best desired effect. 
     Efficient penetration of large multifocal tumors in the liver was achieved in vivo following a single systemic injection ( FIGS.  5 C- 5 D  and  FIGS.  8 A- 8 C ), and this provides strong evidence that even a single injection is capable of delivering a payload to disseminated and well-vascularized tumors, such as HCC. An output with a bystander effect is then able to efficaciously treat these tumors. 
     Example 9. Materials and Method for Examples 1-8 
     Cell lines: HuH-7 cells were purchased from the Health Science Research Resources bank of the Japan Health Sciences Foundation (Cat-#JCRB0403) and cultured at 37° C., 5% CO2 in DMEM, low glucose, GlutaMAX (Life technologies, Cat #21885-025), supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). Hep G2 cells were purchased from ATCC (Cat#HB-8065) and cultured at 37° C., 5% CO2 in RPMI (Gibco A10491-01) supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life Technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). HeLa cells were purchased from ATCC (Cat #CCL-2) and cultured at 37° C., 5% CO2 in DMEM, high glucose (Life technologies, Cat #41966), supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life Technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). Hep3B cells were purchased from ATCC (Cat#HB-8064) and cultured at 37° C., 5% CO2 in DMEM, low glucose, GlutaMAX (Life technologies, Cat #21885-025), supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). HCT-116 cells were purchased from Deutsche Sammlung Von Microorganismen and Zellkulturen (DMZ), DMZ No ACC-581 and cultured at 37° C., 5% CO2 in DMEM GlutaMAX (Life technologies, Cat #31966-021), supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). SW-620 cells were purchased from ATCC (Cat #CCL-227) and cultured at 37° C., 5% CO2 in DMEM GlutaMAX (Life technologies, Cat #31966-021), supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life Technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). LoVo cells were purchased from ATCC (Cat #CCL-229) and cultured at 37° C., 5% CO2 in DMEM GlutaMAX (Life technologies, Cat #31966-021), supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life Technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). A549 cells were purchased from ATCC (Cat #CCL-185) and cultured at 37° C., 5% CO2 in DMEM GlutaMAX (Life technologies, Cat #31966-021), supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life Technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). SH4 cells were purchased from ATCC (Cat #CCL-185) and cultured at 37° C., 5% CO2 in DMEM GlutaMAX (Life technologies, Cat #31966-021), supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life Technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). IGROV1 cells are part of the NCI-60 panel and were obtained by NCI (NIH). The cells were cultured at 37° C., 5% CO2 in RPMI (Gibco A10491-01) supplemented with 10% FBS (Sigma-Aldrich, Cat #F9665 or Life Technologies, Cat #10270106) and 1% Penicillin/Streptomycin solution (Sigma-Aldrich, P4333). 
     Creation of Luciferase and mCitrine Stable Cell Line (HepG2 LC): An HepG2 cell line stably expressing mCitrine and Luciferase (HepG2 LC) was created via TALEN editing of the AAVS locus. 4×10 5  HepG2 cells were seeded in a 6-well plate and transfected after 24 h with a total of 2 μg DNA with Lipofectamine 2000. The transfection mix was composed as follows: 500 ng hAAVS1 1 L TALEN (pIK11), 500 ng hAAVS1 1R TALEN (pIK12) and 1 μg of Luciferase 2A Citrine under the control of a EF1A Promoter (pIK014). Transformed cells were expanded and kept in culture for 3 weeks in order to dilute the expression arising from transient transfection. After 3 weeks the mCitrine +  bulk population (&lt;1%) was sorted using a BD FACS Aria III. The resulting 20.000 cells were seeded in a 24-Well plate in RPMI supplemented with 20% FBS for the first week to facilitate the initial recovery. The cells were cultured and expanded for 2 weeks to select for cells with stable transgene expression and avoid clones prone to be silences. Single mCitrine +  clones were sorted in a 96-well plate, cultured in RPMI supplemented with 20% FBS and expanded. Three different high expressing clones were selected and the best was used for successive experiments. Bioluminescence of the clone was measured for 5 min using the PhotonIMAGER RT (Biospace Laboratories) to confirm Luciferase expression. 
     Viral vector plasmid and virus production: Single-stranded (ss) AAV vectors were produced and purified as previously described. (Paterna 2004, Conway 1999) Briefly, human embryonic kidney cells (HEK293) expressing the simian virus large T-antigen (293T) were cotransfected with polyethylenimine (PEI)-mediated AAV vector plasmids (providing the to-be packaged AAV vector genome), AAV helper plasmids (providing the AAV serotype 2 rep proteins and the cap proteins of the AAV serotype of interest) and adenovirus (AV) helper plasmids pBS-E2A-VA-E4 (Glatzel 2000) in a 1:1:1 molar ratio. 96 to 120 h post transfection HEK293T cells were collected and separated from their supernatant by low-speed centrifugation (15 min at 1500 g/4° C.). AAV vectors released into the supernatant were PEG-precipitated overnight at 4° C. by adding PEG 8000 solution (final: 8% v/v) and NaCl (final: 0.5 M). PEG-precipitation was completed by low-speed centrifugation (60 min at 3488 g/4° C.). Cleared supernatant was discarded and the pelleted AAV vectors resuspended in AAV resuspension buffer (150 mM NaCl, 50 mM Tris-HCl, pH 8.5). HEK293T cells were resuspended in AAV resuspension buffer and lysed by Bertin&#39;s Minilys Homogenizer in combination with 7 mL soft tissue homogenizing CK14 tubes (two 1 min cycles at 5000 rpm/RT, intermitted by &gt;4 min cooling at −20° C.). The crude cell lysate was treated with the BitNuclease endonuclease (75 U/mL, 30 to 90 min at 37° C.) and cleared by centrifugation (10 min at 17 000 g/4° C.). The PEG-pelleted AAV vectors were combined with the cleared lysate and subjected to discontinuous density iodixanol (OptiPrep, Axis-Shield) gradient (isopycnic) ultracentrifugation (2 h 15 min at 365 929 g/15° C.). Subsequently, the iodixanol was removed from the AAV vector containing fraction by three rounds of diafiltration (ultrafiltration) using Vivaspin 20 ultrafiltration devices (100 000 MWCO, PES membrane, Sartorius) and 1× phosphate buffered saline (PBS) supplemented with 1 mM MgCl 2  and 2.5 mM KCl according to the manufacturer&#39;s instructions. The AAV vectors were stored aliquoted at −80° C. Encapsidated viral vector genomes (vg) were quantified using the Qubit 3.0 fluorometer in combination with the Qubit dsDNA HS Assay Kit (both Life Technologies). Briefly, 5 μL of undiluted (or 1:10 diluted) AAV vectors were prepared in duplicate. One sample was heat-denatured (5 min at 95° C.) and the untreated and heat-denatured samples were quantified according to the manufacturer&#39;s instructions. Intraviral (encapsidated) vg/mL were calculated by subtracting the extraviral (nonencapsidated; untreated sample) from the total intra- and extraviral (encapsidated and nonencapsidated; heat-denatured sample). 
     Cell preparation for in vivo injection: HepG2 LC cells were cultured and passaged until 70-80% confluence in T-75 or T-150 flasks. For in vivo injection we used cells with low passage number (passage 12 or less) to minimize silencing of the reporter gene. Cells were detached by removing the growth medium, washing with PBS (10 ml for T-75 or 20 ml for T-150), and dissociating the cells with Trypsin (Gibco, 25200056) (2 ml for T-75 or 6 ml for T-150 Flask) for 5 min at 37° C. The cell suspension was diluted with 8 mL (T-75) or 24 ml (T-150) of PBS, gently resuspended by pipetting, and subsequently filtered in a 50 ml Falcon tube using a 100 μm filter to obtain a single cell suspension. Additional PBS was used to wash the filter 10 ml (T-75) or 20 ml for T-150 further diluting the cells to a total volume of 20 ml (T-75) or 50 ml (T-150). The cell suspension was centrifuged at 498 rpm at 4° C. for 9 min. The cell pellet was washed with 20 ml of PBS and centrifuged at 498 rpm at 4° C. for 6 min two more times to remove any trace of trypsin. The procedure is carried out with one or more flasks and tubes depending on the number of cells needed for the experiment. Each pellet is resuspended in a small amount of PBS (250-300 ul for each pellet) and a small aliquot is diluted (1:50 and 1:100) for manual counting of live cells using Neubauer chamber and trypan blue. At least four independent counts were taken per cell suspension and the average value was used to determine the number of cells to be injected. Cell suspension was inspected visually under the microscope to verify the absence of large clumps. At the end the volume was adjusted with PBS to about 2×10 7  cells/mL. The cell suspension was kept on ice for the duration of the surgeries, given the high cell concentration the cells require resuspension before each injection. In order to minimize manipulation and improve viability the cells are divided in multiple stocks (2-3 tubes). We note that both the presence of cell clumps and the presence of residual trypsin or other cell-dissociation reagents is toxic and potentially life-threatening to the animals. 
     Xenograft mouse liver mouse model: All animal procedures were performed in accordance with the Swiss federal law and institutional guidelines of Eidgenössische Technische Hochschule (ETH) Zurich, and approved by the animal ethics committee of canton Basel-Stadt. Eight to ten-week-old immunodeficient NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ, Charles River, Sulzfeld, Germany) were housed in a specific-pathogen-free facility. To generate the mouse liver tumors derived from human tumor cells, NSG mice were anesthetized with inhalational isoflurane. Using aseptic surgical technique, a left subcostal incision of 1-1.5 cm was made and the spleen was exposed. 10 5  HepG2 cells in 50 μl PBS were injected into the lower lobe of spleen using a 27-gauge needle. Immediately upon removal of the needle the lower pole of the spleen was ligated. A 10-minute draining was allowed for the majority of cells to reach the liver for colonization before the major splenic vasculature was ligated and the spleen is removed. The abdominal incision was then closed with sutures. The tumor growth in mice was monitored by bioluminescence imaging 2-3 times per week (PhotonIMAGER RT, Biospace Lab). 
     In vivo delivery of reporter AAVs and gene expression analysis by fluorescent microscopy and flow cytometry: To visualize circuit output expression in vivo, 2×10 12  vg (viral genomes) of AAVs encoding mCherry output or PBS were administered as a single dose through tail vein 2 weeks after tumor cell transplantation. After 3 weeks mice were euthanized and immediately perfused transcardially with 50-70 mL HBSS containing 10 or 25 U/mL heparin (Sigma-Aldrich) to remove autofluorescent red blood cells. The organs and tissues (liver, lungs, brain, pancreases, skeletal muscles, heart and kidneys) were harvested and fresh tissue slices were prepared and kept on ice in PBS. The expression of mCherry was analyzed immediately by fluorescent microscopy. 
     In vivo delivery of therapeutic AAVs and prodrug treatment: Two weeks after tumor cell inoculation, tumor-bearing mice were first stratified based on tumor burden reflected by bioluminescence intensity (high vs low) and then randomized into various treatment groups to ensure tumor load comparability among groups. 4×10 12  vg (viral genomes) of AAV-circuit constructs or PBS were administered intravenously via two separate injections one week apart. Prodrug GCV (50 mg/kg, InvivoGen) or saline treatment was initiated on day 3 post first AAV injection, mice were injected intraperitoneally once per day for a 2-week duration. Tumor growth was assessed with bioluminescent imaging 2-3 times per week. Mice were monitored with score sheet and euthanized if endpoints were achieved. All mice were terminated after 14 days of prodrug treatment. The livers were harvested for ex vivo bioluminescent imaging analysis of tumor loads. Two weeks after tumor cell inoculation, tumor-bearing mice were first stratified based on tumor burden reflected by bioluminescence intensity (high vs low) and then randomized into various treatment groups to ensure tumor load comparability among groups. 4×10 12  vg (viral genomes) of AAV-circuit constructs or PBS were administered intravenously via two separate injections one week apart. Prodrug GCV (50 mg/kg, InvivoGen) or saline treatment was initiated on day 3 post first AAV injection, mice were injected intraperitoneally once per day for a 2-week duration. Tumor growth was assessed with bioluminescent imaging 2-3 times per week. Mice were monitored with score sheet and euthanized if endpoints were achieved. All mice were terminated after 14 days of prodrug treatment. The livers were harvested for ex vivo bioluminescent imaging analysis of tumor loads. 
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     Other Embodiments 
     All of the features disclosed in this specification may be combined in any combination. Each feature disclosed in this specification may be replaced by an alternative feature serving the same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, each feature disclosed is only an example of a generic series of equivalent or similar features. 
     From the above description, one skilled in the art can easily ascertain the essential characteristics of the present disclosure, and without departing from the spirit and scope thereof, can make various changes and modifications of the disclosure to adapt it to various usages and conditions. Thus, other embodiments are also within the claims. 
     EQUIVALENTS 
     While several inventive embodiments have been described and illustrated herein, those of ordinary skill in the art will readily envision a variety of other means and/or structures for performing the function and/or obtaining the results and/or one or more of the advantages described herein, and each of such variations and/or modifications is deemed to be within the scope of the inventive embodiments described herein. More generally, those skilled in the art will readily appreciate that all parameters, dimensions, materials, and configurations described herein are meant to be exemplary and that the actual parameters, dimensions, materials, and/or configurations will depend upon the specific application or applications for which the inventive teachings is/are used. Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific inventive embodiments described herein. It is, therefore, to be understood that the foregoing embodiments are presented by way of example only and that, within the scope of the appended claims and equivalents thereto, inventive embodiments may be practiced otherwise than as specifically described and claimed. Inventive embodiments of the present disclosure are directed to each individual feature, system, article, material, kit, and/or method described herein. In addition, any combination of two or more such features, systems, articles, materials, kits, and/or methods, if such features, systems, articles, materials, kits, and/or methods are not mutually inconsistent, is included within the inventive scope of the present disclosure. 
     All definitions, as defined and used herein, should be understood to control over dictionary definitions, definitions in documents incorporated by reference, and/or ordinary meanings of the defined terms. 
     All references, patents and patent applications disclosed herein are incorporated by reference with respect to the subject matter for which each is cited, which in some cases may encompass the entirety of the document. 
     The indefinite articles “a” and “an,” as used herein in the specification and in the claims, unless clearly indicated to the contrary, should be understood to mean “at least one.” 
     The phrase “and/or,” as used herein in the specification and in the claims, should be understood to mean “either or both” of the elements so conjoined, i.e., elements that are conjunctively present in some cases and disjunctively present in other cases. Multiple elements listed with “and/or” should be construed in the same fashion, i.e., “one or more” of the elements so conjoined. Other elements may optionally be present other than the elements specifically identified by the “and/or” clause, whether related or unrelated to those elements specifically identified. Thus, as a non-limiting example, a reference to “A and/or B,” when used in conjunction with open-ended language such as “comprising” can refer, in one embodiment, to A only (optionally including elements other than B); in another embodiment, to B only (optionally including elements other than A); in yet another embodiment, to both A and B (optionally including other elements); etc. 
     As used herein in the specification and in the claims, “or” should be understood to have the same meaning as “and/or” as defined above. For example, when separating items in a list, “or” or “and/or” shall be interpreted as being inclusive, i.e., the inclusion of at least one, but also including more than one, of a number or list of elements, and, optionally, additional unlisted items. Only terms clearly indicated to the contrary, such as “only one of” or “exactly one of,” or, when used in the claims, “consisting of,” will refer to the inclusion of exactly one element of a number or list of elements. In general, the term “or” as used herein shall only be interpreted as indicating exclusive alternatives (i.e. “one or the other but not both”) when preceded by terms of exclusivity, such as “either,” “one of,” “only one of,” or “exactly one of.” “Consisting essentially of,” when used in the claims, shall have its ordinary meaning as used in the field of patent law. 
     As used herein in the specification and in the claims, the phrase “at least one,” in reference to a list of one or more elements, should be understood to mean at least one element selected from any one or more of the elements in the list of elements, but not necessarily including at least one of each and every element specifically listed within the list of elements and not excluding any combinations of elements in the list of elements. This definition also allows that elements may optionally be present other than the elements specifically identified within the list of elements to which the phrase “at least one” refers, whether related or unrelated to those elements specifically identified. Thus, as a non-limiting example, “at least one of A and B” (or, equivalently, “at least one of A or B,” or, equivalently “at least one of A and/or B”) can refer, in one embodiment, to at least one, optionally including more than one, A, with no B present (and optionally including elements other than B); in another embodiment, to at least one, optionally including more than one, B, with no A present (and optionally including elements other than A); in yet another embodiment, to at least one, optionally including more than one, A, and at least one, optionally including more than one, B (and optionally including other elements); etc. 
     It should also be understood that, unless clearly indicated to the contrary, in any methods claimed herein that include more than one step or act, the order of the steps or acts of the method is not necessarily limited to the order in which the steps or acts of the method are recited. 
     In the claims, as well as in the specification above, all transitional phrases such as “comprising,” “including,” “carrying,” “having,” “containing,” “involving,” “holding,” “composed of,” and the like are to be understood to be open-ended, i.e., to mean including but not limited to. Only the transitional phrases “consisting of” and “consisting essentially of” shall be closed or semi-closed transitional phrases, respectively, as set forth in the United States Patent Office Manual of Patent Examining Procedures, Section 2111.03. It should be appreciated that embodiments described in this document using an open-ended transitional phrase (e.g., “comprising”) are also contemplated, in alternative embodiments, as “consisting of” and “consisting essentially of” the feature described by the open-ended transitional phrase. For example, if the disclosure describes “a composition comprising A and B,” the disclosure also contemplates the alternative embodiments “a composition consisting of A and B” and “a composition consisting essentially of A and B.”