Patent Publication Number: US-7910112-B2

Title: Feline vaccines against avian influenza

Description:
INCORPORATION BY REFERENCE 
     This application makes reference to U.S. patent application Ser. No. 11/211,983 filed Aug. 25, 2005. 
     The foregoing applications, and all documents cited therein or during their prosecution (“appln cited documents”) and all documents cited or referenced in the appln cited documents, and all documents cited or referenced herein (“herein cited documents”), and all documents cited or referenced in herein cited documents, together with any manufacturer&#39;s instructions, descriptions, product specifications, and product sheets for any products mentioned herein or in any document incorporated by reference herein, are hereby incorporated herein by reference, and may be employed in the practice of the invention. 
     FIELD OF THE INVENTION 
     The present invention encompasses influenza vaccines, in particular avian influenza vaccines. The vaccine may be a recombinant poxvirus vaccine or an inactivated vaccine. The invention also encompasses recombinant poxvirus vectors encoding and expressing avian influenza antigens, epitopes or immunogens which can be used to protect animals, in particular cats, against avian influenza. 
     BACKGROUND OF THE INVENTION 
     Upper respiratory tract diseases (UPTD) are common in felines, especially domestic cats. These diseases exhibit influenza-like symptoms, including fever, nasal discharge, sneezing, coughing, sinusitis and bronchitis, and can potentially be fatal. The most common agents for UPTD include chlamydia, feline herpesvirus-1, feline calicivirus, and  Bordetella bronchiseptica . More recently, felines have displayed susceptibility of infection to influenza viruses, especially the avian influenza virus (AIV). 
     AIV is an RNA virus belonging to the family of Orthomyxoviridae, and is classified as a type A influenza virus, which relates its nucleoprotein and membrane proteins. AIV has a lipid envelope that features two distinct glycoproteins: hemagglutinin (HA), which facilitates entry of the virus into the host cells, and neuraminidase (NA), which assists in the release of progeny virus from infected cells (de Jong et al., 2006). Thus far, 16 HA and 9 NA have been detected and can exist in varying combinations (Olsen et al., 2006), thereby forming subtypes of AIV that are based upon these antigenic differences. The H5N1 subtype (virus featuring HA 5 and NA 1) has specifically been associated with recent outbreaks in Asia, Russia, the Middle East, Europe and Africa, and is responsible for the growing concern of avian influenza infection of felids. 
     Reports regarding avian influenza infection of domestic cats and zoo felids emerged during the 2003 to 2004 avian influenza outbreak in Asia (Keawcharoen et al 2004). These reports described over 150 deaths of tigers and leopards as a result of feeding on raw chicken carcasses that were infected with the avian influenza. These felids developed lesions in the lungs, resulting in congestion and hemorrhaging, moderate meningoencephalitis, and mutifocal necrotizing hepatitis. Since then, scientists have confirmed that felines are susceptible to avian influenza infection via intratracheal injection, consumption of virus-infected chickens, and horizontal transmission through regular contact (Kuiken et al., 2004). The infected cats initially developed such symptoms as raised body temperature, conjunctivitis, and labored breathing, which eventually progressed to severe diffuse alveolar damage and death, although the virus can replicate in the respiratory tract without inducing any signs of the disease (Hinshaw et al. 1981). Further studies revealed that experimentally-infected cats displayed a presence of the virus in both respiratory and extra-respiratory organs, and excreted the virus through both the respiratory and digestive tracts (Rimmelzwaan et al., 2006). In addition, numerous new reports have described infection of domestic cats in Europe and Asia (Butler, 2006). 
     Considering the susceptibility of felines to AIV and their ability to excrete the virus into their surroundings, a method of preventing AIV infection and protecting felines is essential. The urgency is compounded given the possibility that feline infection may play a role in the epidemiology of AIV in poultry, humans, and other species (Influenza team, 2006; Kuiken et al., 2006). Accordingly, there is a need for an effective vaccine against influenza in felines. 
     Citation or identification of any document in this application is not an admission that such document is available as prior art to the present invention. 
     SUMMARY OF THE INVENTION 
     The present invention encompasses avian influenza immunological compositions, which may be a recombinant avian influenza immunological composition or an inactivated avian influenza immunological composition. 
     The present invention also encompasses avian influenza vaccines, which may be a recombinant avian influenza vaccine or an inactivated avian influenza vaccine. 
     Furthermore, the present invention encompasses influenza vaccines wherein the vaccine comprises one or more of an inactivated feline influenza isolate, an inactivated avian influenza isolate, or mixtures thereof. 
     In an embodiment wherein the avian influenza immunological composition or vaccine is a recombinant immunological composition or vaccine, advantageously, the composition or vaccine comprising a recombinant viral vector and a pharmaceutical or veterinary acceptable excipient, carrier or vehicle; the recombinant viral vector is an avipox expression vector which may comprise a polynucleotide encoding an influenza polypeptide, antigen, epitope or immunogen. The influenza polypeptide, antigen, epitope or immunogen, may be a hemagglutinin, matrix protein, neuraminidase, nonstructural protein, nucleoprotein, polymerase or any fragment thereof. 
     In an advantageous embodiment, the avian influenza polypeptide, antigen, epitope or immunogen may be derived from a feline infected with influenza. For example, but not by limitation, influenza virus may be isolated from the broncho alveolar lavage and/or lung tissues of an affected felid. Isolation and characterization of the nucleotide sequence of the influenza infecting the felid may be done by routine experimentation by a person of ordinary skill in the art. 
     The avian influenza polypeptide, antigen, epitope or immunogen may be isolated from an avian influenza. 
     The avipox expression vector may be an attenuated avipox expression vector. In one embodiment, the avipox expression vector may be a fowlpox vector, advantageously TROVAC. In another embodiment, the avipox expression vector may be a canarypox vector, advantageously ALVAC. The influenza antigen, epitope or immunogen may be a hemagglutinin, such as H5. The fowlpox vector may be vFP89 or vFP2211. The canarypox vector may be vCP2241. 
     The present invention also encompasses an inactivated influenza immunological composition or vaccine. The inactivated influenza immunological composition or vaccine may be an inactivated influenza. In another embodiment, the inactivated influenza immunological composition or vaccine may be an avian influenza. The immunological composition o vaccine may be inactivated with formalin or beta-propiolactone. 
     The invention also relates to method of eliciting an immune response against influenza in a Felidae, in particular a cat, which may comprise administering a formulation comprising any one of the above recombinant influenza immunological composition or vaccine, or inactivated immunological composition or vaccine, and a pharmaceutically or veterinarily acceptable carrier, excipient or vehicle in an effective amount for eliciting an immune response. In an advantageous embodiment, an adjuvant may be added. The adjuvant may be aluminum hydroxide, aluminum phosphate, a carbomer or an oil-in-water-emulsion and optionally may comprise CpG. Advantageously, the administration may be subcutaneous intramuscular or transdermal with a needle-free injector. 
     The invention further relates to method of inducing a immune response against influenza in a Felidae, in particular in a cat, which may comprise administering a formulation comprising any one of the above recombinant influenza immunological compositions or vaccines, or inactivated immunological compositions or vaccines, and a pharmaceutically or veterinarily acceptable carrier, excipient or vehicle in an effective amount for inducing a immune response. In an advantageous embodiment, an adjuvant may be added. The adjuvant may be aluminum hydroxide, aluminum phosphate, a carbomer or an oil-in-water-emulsion and optionally may comprise CpG. Advantageously, the administration may be subcutaneous or intramuscular. 
     The invention further comprises relates to the inducement or elicication of an immune response against influenza in a Felidae wherein the immune response induced or elicited is a protective immune response. 
     The invention further encompasses a kit for performing a method of eliciting or inducing an immune response which may comprise any one of the recombinant influenza immunological compositions or vaccines, or inactivated immunological compositions or vaccines, and instructions for performing the method. 
     Accordingly, it is an object of the invention to not encompass within the invention any previously known product, process of making the product, or method of using the product such that Applicants reserve the right and hereby disclose a disclaimer of any previously known product, process, or method. It is further noted that the invention does not intend to encompass within the scope of the invention any product, process, or making of the product or method of using the product, which does not meet the written description and enablement requirements of the USPTO (35 U.S.C. §112, first paragraph) or the EPO (Article 83 of the EPC), such that Applicants reserve the right and hereby disclose a disclaimer of any previously described product, process of making the product, or method of using the product. 
     It is noted that in this disclosure and particularly in the claims and/or paragraphs, terms such as “comprises”, “comprised”, “comprising” and the like can have the meaning attributed to it in U.S. Patent law; e.g., they can mean “includes”, “included”, “including”, and the like; and that terms such as “consisting essentially of” and “consists essentially of” have the meaning ascribed to them in U.S. Patent law, e.g., they allow for elements not explicitly recited, but exclude elements that are found in the prior art or that affect a basic or novel characteristic of the invention. 
     These and other embodiments are disclosed or are obvious from and encompassed by, the following Detailed Description. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The following detailed description, given by way of example, but not intended to limit the invention solely to the specific embodiments described, may best be understood in conjunction with the accompanying drawing, in which: 
         FIG. 1  illustrates the generation of vFP2211, in which plasmid pJY1394.1, containing the synthetic AIV H5 HA insert, and a fragment of the Fowlpox genome at the F8 locus generates vFP2211 through in vitro recombination. 
         FIG. 2  illustrates the fragment of vFP2211, indicating the positions of the primers for amplifying the AIV probe and for PCR amplification of the F8 arms and insert. 
         FIG. 3  illustrates a theoretical restriction enzyme gel for the genomic DNA of vFP2211. 
         FIG. 4  illustrates a gel from electrophoresis of genomic DNA extracted from vFP2211 and digested with BamHI, Hind III, and Pst I. 
         FIG. 5  illustrates a gel from Western blot analysis indicating the correct insertion of synthetic AIV H5 HA into the C5 locus. 
         FIG. 6  illustrates the results of an immunoplaque assay of vFP2211, indicating that the homogeneity of the population was 100%. 
         FIG. 7  illustrates a comparison of nucleotide sequences between the wild type H5 HA without cleavage site (CK/Indonesia/2003) and the synthetic AIV H5 HA without cleavage site from plasmid pCR-Script/HA-CK/Indonesia/03-(modified)-avipox (SEQ ID NOS 23 &amp; 15). 
         FIG. 8  illustrates a comparison of amino acid sequences between the wild type H5 HA without cleavage site (CK/Indonesia/2003) and the synthetic AIV H5 HA without cleavage site from plasmid pCR-Script/HA-CK/Indonesia/03-(modified)-avipox (SEQ ID NOS 16 &amp; 24). 
         FIG. 9  illustrates the construction of the ALVAC plasmid pLH1852.5, in which the expression cassette of H6p-AIV synthetic H5 HA is isolated by digestion of the plasmid pJY1394.1 and ligated to Eco DNA digested pALVAC C5H6p donor (pCXL148.2). 
         FIG. 10  illustrates the ALVAC plasmid pLH1852.5. 
         FIG. 11  illustrates the generation of vCP2241, in which ALVAC plasmid pLH1852.5, containing the AIV synthetic H5 HA insert, and a fragment of the ALVAC genome at the C5 locus generates vCP2241 through in vitro recombination. 
         FIG. 12  illustrates a theoretical restriction enzyme gel for the genomic DNA of vCP2241.4.1.1.1. 
         FIG. 13  illustrates a gel from electrophoresis of genomic DNA extracted from vCP2241.4.1.1.1 and digested with BamHI, Hind III, and Pst I. 
         FIG. 14  illustrates a gel from Southern blot analysis indicating the expression of the H5 HA protein. 
         FIG. 15  illustrates a gel from Western blot analysis indicating the correct insertion of AIV synthetic H5 HA into the C5 locus. 
         FIG. 16  illustrates the results of an immunoplaque assay of vCP2241.4.1.1.1, indicating that the homogeneity of the population was 100%. 
         FIG. 17  illustrates the fragment of vCP2241, indicating the positions of the primers for amplifying the AIV probe and for PCR amplification of the C5 arms and insert. 
         FIG. 18  illustrates the HI antibody responses to H5N1 AIV (A/Vietnam/1194/04 antigens in cats vaccinated with either TROVAC fowlpox virus expressing the H5 gene from A/Turkey/Ireland/1378/83 (vFP89), TROVAC fowlpox virus expressing the H5 gene from A/Chicken/Indonesia/03 (vFP2211), or ALVAC canarypox virus expressing the H5 gene from A/Chicken/Indonesia/03 (vCP2241). 
         FIG. 19  illustrates the HI antibody responses to, H5N8 AIV (A/Turkey/Ireland/1378/83) NIBRG14 strain) antigens in cats vaccinated with either TROVAC fowlpox virus expressing the H5 gene from A/Turkey/Ireland/1378/83 (vFP89), TROVAC fowlpox virus expressing the H5 gene from A/Chicken/Indonesia/03 (vFP2211), or ALVAC canarypox virus expressing the H5 gene from A/Chicken/Indonesia/03 (vCP2241). 
         FIG. 20  illustrates the HI antibody responses to H5N1 AIV (A/Vietnam/1194/04, NIBRG14 strain antigens in cats vaccinated with an inactivated H5N9 AIV (A/Chicken/Italy/22A/98) at dosages of 512UHA/dose and 1536UHA/dose) 
         FIG. 21  illustrates the HI antibody responses to H5N9 AIV (A/Turkey/Wisconsin/68) antigens in cats vaccinated with an inactivated H5N9 AIV (A/Chicken/Italy/22A/98) at dosages of 512UHA/dose and 1536UHA/dose) 
     
    
    
     DETAILED DESCRIPTION 
     The present invention is based, in part, on Applicants&#39; studies demonstrating a recombinant fowlpox and canarypox expressing avian influenza HA is immunogenic in cats. 
     The present invention encompasses any influenza polypeptide, antigen, epitope or immunogen that elicits an immunogenic response in an animal, advantageously a vertebrate, more advantageously a Felidae, even more advantageously a cat. The influenza polypeptide, antigen, epitope or immunogen may be any influenza polypeptide, antigen, epitope or immunogen, such as, but not limited to, a protein, peptide or fragment thereof, that elicits, induces or stimulates a response in an animal, advantageously a vertebrate, a Felidae more advantageously a cat. 
     In an advantageous embodiment, the influenza polypeptide, antigen, epitope or immunogen is derived from a feline infected with influenza. For example, but not by limitation, influenza virus may be isolated from the broncho alveolar lavage and/or lung tissues of an affected felid. Isolation and characterization of the nucleotide sequence of the influenza infecting the felid may be done by routine experimentation by a person of ordinary skill in the art. 
     In another advantageous embodiment, the influenza polypeptide, antigen, epitope or immunogen may be derived from an avian infected with influenza or an avian influenza strain. Advantageously, the avian influenza antigen, epitope or immunogen is a hemagglutinin (HA) (e.g., HA precursor, H1, H2, protein, matrix protein (e.g., matrix protein M1 or M2), neuraminidase, nonstructural (NS) protein (e.g., NS1 or NS2), nucleoprotein (NP) and polymerase (e.g., PA polymerase, PB1 polymerase 1 or PB2 polymerase 2). 
     Examples of avian influenza strains that may be used in methods of the present invention include, but are not limited to, turkey influenza virus strain A/Turkey/Ireland/1378/83 (H5N8) (see, e.g., Taylor et al., 1988b), turkey influenza virus strain A/Turkey/England/63 (H7N3) (see, e.g., Alexander et al., 1979; Rott et al., 1979; Horimoto et al., 2001), turkey influenza virus strain A/Turkey/England/66 (H6N2) (see, e.g., Alexander et al., 1979), A/Turkey/England/69 (H7N2) (see, e.g., Alexander et al., 1979; Horimoto et al., 2001), A/Turkey/Scotland/70 (H6N2) (see, e.g., Banks et al., 2000; Alexander et al., 1979), turkey influenza virus strain A/Turkey/England/N28/73 (H5N2) (see, e.g., Alexander et al., 1979), turkey influenza virus strain A/Turkey/England/110/77 (H6N2) (see, e.g., Alexander et al., 1979), turkey influenza virus strain A/Turkey/England/647/77 (H1N1) (see, e.g., Alexander et al., 1979; Karasin et al., 2002)), turkey influenza virus strain A/turkey/Ontario/7732/66 (H5N9) (see, e.g., Slemons et al., 1972; Philpott et al., 1989), turkey influenza virus strain A/Turkey/England/199/79 (H7N7) (see, e.g., Horimoto et al., 2001), turkey influenza virus strain A/Turkey/Ontario/7732/66 (H5N9) (see, e.g., Horimoto et al., 2001; Panigrahy et al., 1996), turkey influenza virus strain A/Turkey/Ireland/1378/85 (H5N8) (see, e.g., Horimoto et al., 2001; Walker et al., 1993), turkey influenza virus strain A/Turkey/England/50-92/91 (H5N1) (see, e.g., Horimoto et al., 2001; Howard et al., 2006), turkey influenza virus strain A/Turkey/Wisconsin/68 (H5N9), turkey influenza virus strain A/Turkey/Masschusetts/65 (H6N2), turkey influenza virus strain A/Turkey/Oregon/71 (H7N3), (see, e.g., Orlich et al., 1990), turkey influenza virus strain A/Turkey/Ontario/6228/67 (H8N4), turkey influenza virus strain A/Turkey/Wisconsin/66 (H9N2), (see, e.g., Zakstel&#39;skaia et al., 1977), turkey influenza virus strain A/Turkey/England/647/77 (H1N1) (see, e.g., Karasin et al., 2002; Alexander et al., 1979), turkey influenza virus strain A/Turkey/Ontario/6118/68 (H8N4) (see, e.g., Blok et al., 1982), turkey influenza virus strain A/Tur/Ger 3/91 (see, e.g., Zakay-Rones et al., 1995), turkey influenza virus strain A/Turkey/Minnesota/833/80 (H4N2) (see, e.g., Gubareva et al., 1997) chicken influenza virus strain A/Chicken/Indonesia/03 (H5N1), chicken influenza virus strain A/Chicken/FPV/Rostock/1934 (see, e.g., Ohuchi et al., 1994), chicken influenza virus strain A/Chicken/Texas/298313/04 (see, e.g., Lee et al., 2005), chicken influenza virus strain A/Chicken/Texas/167280-4-/02 (see, e.g., Lee et al., 2005), chicken influenza virus strain A/Chicken/Hong Kong/220/97 (see, e.g., Perkins et al., 2001), chicken influenza virus strain A/Chicken/Italy/8/98 (see, e.g., Capua et al., 1999), chicken influenza virus strain A/Chicken/Victoria/76 (H7N7) (see, e.g., Zambon, 2001; Nestorowicz et al., 1987), chicken influenza virus strain A/Chicken/Germany/79 (H7N7) (see, e.g., Rohm et al., 1996), chicken influenza virus strain A/Chicken/Scotland/59 (H5N1) (see, e.g., Horimoto et al., 2001; De et al., 1988; Wood et al., 1993), chicken influenza virus strain A/Chicken/Pennsylvania/1370/83 (H5N2) (see, e.g., Bean et al., 1985; van der Goot et al., 2002), chicken influenza virus strain A/Chicken/Queretaro-19/95 (H5N2) (see, e.g., Horimoto et al., 2001; Garcia et al., 1998), chicken influenza virus strain A/Chicken/Queretaro-20/95 (H5N2) (see, e.g., Horimoto et al., 2001), chicken influenza virus strain A/Chicken/Hong Kong/258/97 (H5N1) (see, e.g., Horimoto et al., 2001; Webster, 1998), chicken influenza virus strain A/Chicken/Italy/1487/97 (H5N2) (see, e.g., Horimoto et al., 2001), chicken influenza virus strain A/Chicken/Leipzig/79 (H7N7) (see, e.g., Horimoto et al., 2001; Rohm et al., 1996), chicken influenza virus strain A/Chicken/Victoria/85 (H7N7) (see, e.g., Horimoto et al., 2001), chicken influenza virus strain A/Chicken/Victoria/92 (H7N3) (see, e.g., Horimoto et al., 2001), chicken influenza virus strain A/Chicken/Queensland/95 (H7N3) (see, e.g., Horimoto et al., 2001), chicken influenza virus strain A/Chicken/Pakistan/1369/95 (H7N2) (see, e.g., Horimoto et al., 2001), chicken influenza virus strain A/Chicken/Pakistan/447-4/95 (H7N3) (see, e.g., Horimoto et al., 2001), chicken influenza virus strain A/Chicken/HK/G9/97 (H9N2) (see, e.g., Leneva et al., 2001), chicken influenza virus strain A/Chicken/Nakom-Patom/Thailand/CU-K2/2004(H5N1) (see, e.g., Anwar et al., 2006; Viseshakul et al., 2004), chicken influenza virus strain A/Chicken/Hong Kong/31.2/2002 (H5N1), (see, e.g., Anwar et al., 2006;), chicken influenza virus strain A/Chicken/Vietnam/C58/04 (H5N1), (see, e.g., Anwar et al., 2006;), chicken influenza virus strain A/Chicken/Vietnam/38/2004(H5N1). (see, e.g., Anwar et al., 2006), chicken influenza virus strain A/Chicken/Alabama/7395/75 (H4N8), (see, e.g., Swayne et al., 1994), chicken influenza virus strain A/Chicken/Germany/N/49 (H10N7), (see, e.g., Yamane et al., 1981), chicken influenza virus strain A/Chicken/Beijing/1/94 (H9N2) (see, e.g., Karasin et al., 2002), chicken influenza virus strain A/Chicken/Hong Kong/G23/97 (H9N2) (see, e.g., Karasin et al., 2002), chicken influenza virus strain A/Chicken/Pennsylvania/8125/83 (H5N2) (see, e.g., Karasin et al., 2002; Shortridge et al., 1998), chicken influenza virus strain A/Chicken/Hong Kong/97 (H5N1) (see, e.g., Chen et al., 2003), duck influenza virus strain A/Duck/Anyang/AVL-1/01 (see, e.g., Tumpey et al., 2002), duck influenza virus strain A/Duck/New York/17542-4/86 (H9N1) (see, e.g., Banks et al., 2000), duck influenza virus strain A/Duck/Alberta/28/76 (H4N6) (see, e.g., Blok et al., 1982), duck influenza virus strain A/Duck/Nanchang/4-165/2000 (H4N6) (see, e.g., Liu et al., 2003), duck influenza virus strain A/Duck/Germany/49 (H10N7) (see, e.g., Blok et al., 1982), duck influenza virus strain A/Black Duck/Australia/702/78 (H3N8) (see, e.g., Blok et al., 1982), duck influenza virus strain A/Duck/Vietnam/11/2004 (H5N1), (see, e.g., Anwar et al., 2006), duck influenza virus strain A/Duck/Alberta/60/76 (H12N5), (see, e.g., Baez et al., 1981), duck influenza virus strain A/Duck/Hong Kong/196/77 (H1) (see, e.g., Karasin et al., 2002; Kanegae et al., 1994), duck influenza virus strain A/Duck/Wisconsin/1938/80 (H1N1) (see, e.g., Karasin et al., 2002), duck influenza virus strain A/Duck/Bavaria/2/77 (H1N1) (see, e.g., Karasin et al., 2002; Ottis et al., 1980), duck influenza virus strain A/Duck/Bavaria/1/77 (H1N1) (see, e.g., Ottis et al., 1980), duck influenza virus strain A/Duck/Australia/749/80 (H1N1) (see, e.g., Karasin et al., 2002), duck influenza virus strain A/Duck/Hong Kong/Y280/97 (H9N2) (see, e.g., Karasin et al., 2002; Guan et al., 2000), duck influenza virus strain A/Duck/Alberta/35/76 H1N1) (see, e.g., Austin et al., 1990), avian influenza virus strain A/Mallard duck/Gurjev/263/82 (H14N5), (see, e.g., Kawaoka et al., 1990), avian influenza virus strain A/Mallard duck/PA/10218/84 (H5N2) (see, e.g., Smimov et al., 2000), avian influenza virus strain A/Mallard duck/Astrakhan/244/82 (H14N6) (see, e.g., Karasin et al., 2002), goose influenza virus strain A/Goose/Guangdong/1/96 (see, e.g., Xu et al., 1999), goose influenza virus strain A/Goose/Leipzig/137-8/79 (H7N7) (see, e.g., Horimoto et al., 2001), goose influenza virus strain A/Goose/Hong Kong/W222/97 (H6N7) (see, e.g., Chin et al., 2002), goose influenza virus strain A/Goose/Leipzig/187-7/79 (H7N7) (see, e.g., Horimoto et al., 2001), goose influenza virus strain A/Goose/Leipzig/192-7/79 (H7N7) (see, e.g., Horimoto et al., 2001), avian influenza virus strain A/Env/HK/437-4/99 (see, e.g., Cauthen et al., 2000), avian influenza virus strain A/Env/HK/437-6/99 (see, e.g., Cauthen et al., 2000), avian influenza virus strain A/Env/HK/437-8/99 (see, e.g., Cauthen et al., 2000), avian influenza virus strain A/Env/HK/437-10/99, (see, e.g., Cauthen et al., 2000), avian influenza virus strain A/Fowl plague virus strain/Dutch/27 (H7N7) (see, e.g., Horimoto et al., 2001; Carter et al., 1982), avian influenza virus strain A/Fowl plague virus strain/Dobson/27 (H7N7) (see, e.g., Horimoto et al., 2001), avian influenza virus strain A/Fowl plague virus strain/Rostock/34 (H7N1) (see, e.g., Horimoto et al., 2001; Takeuchi et al., 1994), avian influenza virus strain A/Fowl plague virus strain/Egypt/45 (H7N1) (see, e.g., Horimoto et al., 2001), avian influenza virus strain A/Fowl plague virus strain/Weybridge (H7N7) (see, e.g., Tonew et al., 1982), avian influenza virus strain A/Tern/South Africa/61 (H5N3) (see, e.g., Horimoto et al., 2001; Perkins et al., 2002; Walker et al., 1992), avian influenza virus strain A/Tern/Australia/G70C/75 (H11N9) (see, e.g., Pruett et al., 1998), avian influenza virus strain A/Quail/Vietnam/36/04(H5N1). (see, e.g., Anwar et al., 2006), avian influenza virus strain A/Gull/Maryland/704/77 (H13N6), (see, e.g., Iamnikova et al., 1989), avian influenza virus strain A/Black-headed gull/Sweden/5/99 (H16N3) (see, e.g., Fouchier et al., 2005), avian influenza virus strain A/Herring gull/DE/677/88 (H2N8) (see, e.g., Saito et al., 1993), avian influenza virus strain A/Swan/Italy/179/06 (H5N1) (see, e.g., Terregino et al., 2006), avian influenza virus strain A/Hong Kong/156/97 (A/HK/156/97) (see, e.g., Leneva et al., 2001; Claas et al., 1998; Cauthen et al., 2000), avian influenza virus strain A/Quail/HK/G1/97 (H9N2) (see, e.g., Leneva et al., 2001), avian influenza virus strain A/Quail/Hong Kong/AF157/93 (H9N2) (see, e.g., Karasin et al., 2002), avian influenza virus strain A/Teal/HK/W312/97 (H6N1) (see, e.g., Leneva et al., 2001), avian influenza virus strain A/Shearwater/West Australia/2576/79 (H15N9) (see, e.g., Rohm et al., 1996), avian influenza virus strain A/Shearwater/Australia/72 (H6N5) (see, e.g., Harley et al., 1990), avian influenza virus strain A/Hong Kong/212/03 (see, e.g., Shinya et al., 2005), avian influenza virus strain A/England/321/77 (H3N2) (see, e.g., Hauptmann et al., 1983), avian pandemic influenza A viruses of avian origin (see, e.g., Audsley et al., 2004) avian H5N1 influenza virus, avian H7N1 influenza strain (see, e.g., Foni et al., 2005), avian H9N2 influenza virus (see, e.g., Leneva et al., 2001), and avian influenza virus, cold-adapted (ca) and temperature sensitive (ts) master donor strain, A/Leningrad/134/17/57 (H2N2) (see, e.g., Youil et al., 2004), the disclosures of which are incorporated by reference. 
     Other influenza strains that may be used in methods of the present invention include, but are not limited to, equine influenza virus (A/Equi 2 (H3N8), Newmarket 1/93) (see, e.g., Mohler et al., 2005; Nayak et al., 2005), equine-2 influenza virus (EIV; subtype H3N8) (see, e.g., Lin et al., 2001), equine-2 influenza virus, A/Equine/Kentucky/1/91 (H3N8) (see, e.g., Youngner et al., 2001), equine influenza virus strain A/Equine/Berlin/2/91 (H3N8) (see, e.g., Ilobi et al., 1998), equine influenza virus strain A/Equine/Cambridge/1/63 (H7N7) (see, e.g., Gibson et al., 1992), equine influenza virus strain A/Equine/Prague/1/56 (H7N7) (see, e.g., Karasin et al., 2002; Appleton et al., 1989), equine influenza virus strain A/Eq/Kentucky/98 (see, e.g., Crouch et al., 2004), equine influenza virus strain A/Equi 2 (Kentucky 81) (see, e.g., Short et al., 1986; Horner et al., 1988), equine influenza virus strain A/Equine/Kentucky/1/81 (Eq/Ky) (see, e.g., Breathnach et al., 2004), equine influenza virus strain A/Equine/Kentucky/1/81 (H3N8) (see, e.g., Olsen et al., 1997; Morley et al., 1995; Ozaki et al., 2001; Sugiura et al., 2001; Goto et al., 1993), equine influenza virus strain A/Equine/Kentucky/1/91 (H3N8) (see, e.g., Youngner et al., 2001), equine influenza virus strain A/Equine/Kentucky/1277/90 (Eq/Kentucky) (see, e.g., Webster et al., 1993), equine influenza virus strain A/Equine/Kentucky/2/91 (H3N8) (see, e.g., Donofrio et al., 1994), equine influenza virus strain A/Equine/Kentucky/79 (H3N8) (see, e.g., Donofrio et al., 1994), equine influenza virus strain A/Equine/Kentucky/81 (see, e.g., Sugiura et al., 2001), equine influenza virus strain A/Equine/Kentucky/91 (H3N8) (see, e.g., Gross et al., 1998), equine influenza virus strain A/Equine-2/Kentucky/95 (H3N8) (see, e.g., Heldens et al., 2004) and equine influenza virus strain A/Equine-2/Kentucky/98 (see, e.g., Chambers et al., 2001), equine influenza virus strain A/Eq/Newmarket/1/77 (see, e.g., Lindstrom et al., 1998), equine influenza virus strain A/Eq/Newmarket/5/03 (see, e.g., Edlund Toulemonde et al., 2005), equine influenza virus strain A/Equi 2 (H3N8), Newmarket 1/93 (see, e.g., Mohler et al., 2005; Nayak et al., 2005), equine influenza virus strain A/Equi-2/Newmarket-1/93 (see, e.g., Heldens et al., 2002), equine influenza virus strain A/Equine/Newmarket/2/93 (see, e.g., Wattrang et al., 2003), equine influenza virus strain A/Equine/Newmarket/79 (H3N8) (see, e.g., Duhaut et al., 2000; Noble et al., 1994; Duhaut et al., 1998; Hannant et al., 1989; Hannant et al., 1989; Hannant et al., 1988; Richards et al., 1992; Heldens et al., 2004), equine influenza virus strain A/Equine/Newmarket/1/77 (H7N7) (see, e.g., Goto et al., 1993; Sugiura et al., 2001) and equine influenza virus strain A/Equine-2/Newmarket-2/93 (see, e.g., Heldens et al., 2004), equine influenza virus strain A/Eq/Miami/63 (H3N8) (see, e.g., van Maanen et al., 2003), A/Equi 1 (Prague strain) (see, e.g., Horner et al., 1988; Short et al., 1986), equine influenza virus strain A/Equi 2 (Miami) (see, e.g., Short et al., 1986), equine influenza virus strain A/Equi-1/Prague/56 (Pr/56) (see, e.g., Heldens et al., 2002), equine influenza virus strain A/Equi-2/Suffolk/89 (Suf/89) (see, e.g., Heldens et al., 2002), equine influenza virus strain A/Equine 2/Sussex/89 (H3N8) (see, e.g., Mumford et al., 1994), equine influenza virus strain A/Equine/Sussex/89 (see, e.g., Wattrang et al., 2003), equine influenza virus strain A/Equine-2/Saskatoon/90 (see, e.g., Chambers et al., 2001), equine influenza virus strain A/Equine/Prague/1/56 (H7N7) (see, e.g., Donofrio et al., 1994; Morley et al., 1995), equine influenza virus strain A/Equine/Miami/1/63 (H3N8) (see, e.g., Morley et al., 1995; Ozaki et al., 2001; Thomson et al., 1977; Mumford et al., 1988; Donofrio et al., 1994; Mumford et al., 1983), A/Aichi/2/68 (H3N2) (see, e.g., Ozaki et al., 2001), equine influenza virus strain A/Equine/Tokyo/2/71 (H3N8) (see, e.g., Goto et al., 1993), equine influenza virus strain A/Eq/LaPlata/1/88 (see, e.g., Lindstrom et al., 1998), equine influenza virus strain A/Equine/Jilin/1/89 (Eq/Jilin) (see, e.g., Webster et al., 1993), equine influenza virus strain A/Equine/Alaska/1/91 (H3N8) (see, e.g., Webster et al., 1993), equine influenza virus strain A/Equine/Saskatoon/1/91 (H3N8) (see, e.g., Morley et al., 1995), equine influenza virus strain A/Equine/Rome/5/91 (H3N8) (see, e.g., Sugiura et al., 2001), equine influenza virus strain A/Equine/La Plata/1/93 (H3N8) (see, e.g., Ozaki et al., 2001), equine influenza virus strain A/Equine/La Plata/1/93 (LP/93) (see, e.g., Sugiura et al., 2001), equine influenza virus strain A/Eq/Holland/1/95 (H3N8) (see, e.g., van Maanen et al., 2003) and equine influenza virus strain A/Eq/Holland/2/95 (H3N8) (see, e.g., van Maanen et al., 2003), human influenza virus A(H3N2) isolates (see, e.g., Abed et al., 2002), human influenza virus A/Memphis/1/71 (H3N2) (see, e.g., Suzuki et al., 1996), human influenza virus A/Nanchang/933/95 (H3N2) virus (see, e.g., Scholtissek et al., 2002), human influenza virus A/PR/8/34 (H1N1) virus (see, e.g., Scholtissek et al., 2002), human influenza virus A/Singapore/57 (H2N2) virus (see, e.g., Scholtissek et al., 2002), influenza virus A (see, e.g., Chare et al., 2003), influenza virus A/HK/213/03 (see, e.g., Guan et al., 2004; Anwar et al., 2006), influenza virus strain A/HK/483/97 (see, e.g., Cheung et al., 2002), influenza virus strain A/HK/486/97 (see, e.g., Cheung et al., 2002), influenza virus strain A/Thailand/5(KK-494)/2004 (H5N1). (see, e.g., Anwar et al., 2006), influenza virus strain A PR/8/34 (PR8) virus strain (H1N1 subtype) (see, e.g., Mantani et al., 2001), influenza virus strain A/Aichi/2/68(H3N2) (see, e.g., Miyamoto et al., 1998), influenza virus strain A/Ann Arbor/6/60 cold-adapted virus strain (see, e.g., Treanor et al., 1994), influenza virus strain A/Beijing 32/92 (H3N2) (see, e.g., Zakay-Rones et al., 1995), influenza virus strain A/Charlottesville/31/95 (H1N1) (see, e.g., Gubareva et al., 2002), influenza virus strain A/Kawasaki/86 (H1N1) virus strain (see, e.g., Staschke et al., 1998), influenza virus strain A/Korea/82 (H3N2) (see, e.g., Treanor et al., 1994), influenza virus strain A/Leningrad/134/57 (see, e.g., Egorov et al., 1998), influenza virus strain A/NWS/33 (H1N1) (see, e.g., Sidwell et al., 1998), influenza virus strain A/PR/8/34(H1N1) (see, e.g., Miyamoto et al., 1998), influenza virus strain A/PR8/34 (see, e.g., Nunes-Correia et al., 1999; Tree et al., 2001), influenza virus strain A/Puerto Rico (PR)/8/34 (see, e.g., Egorov et al., 1998), influenza virus strain A/Puerto Rico/8-Mount Sinai (see, e.g., Mazanec et al., 1995), influenza virus strain A/Shangdong 9/93 (H3N2) (see, e.g., Zakay-Rones et al., 1995; Sidwell et al., 1998), influenza virus strain A/Shingapol/1/57(H2N2) (see, e.g., Miyamoto et al., 1998), influenza virus strain A/Singapore 6/86 (H1N1) (see, e.g., Zakay-Rones et al., 1995), influenza virus strain A/Singapore/1/57 (H2N2) (see, e.g., Bantia et al., 1998), influenza virus strain A/Texas 36/91 (H1N1) (see, e.g., Zakay-Rones et al., 1995), influenza virus strain A/Texas/36/91 (H1N1) virus strain (see, e.g., Gubareva et al., 2001; Halperin et al., 1998), influenza virus strain A/Texas/36/91(H1N1) (see, e.g., Hayden et al., 1994), influenza virus strain A/Udorn/72 virus infection (see, e.g., Shimizu et al., 1999), influenza virus A/Victoria/3/75 (H3N2) (see, e.g., Sidwell et al., 1998), influenza virus A/Virginia/88(H3N2) (see, e.g., Hayden et al., 1994), influenza virus A/WSN/33 (H1N1) (see, e.g., Lu et al., 2002), influenza virus A/WSN/33 (see, e.g., Gujuluva et al., 1994), influenza virus B (see, e.g., Chare et al., 2003), influenza virus B/Ann Arbor 1/86 (see, e.g., Zakay-Rones et al., 1995), influenza virus B/Harbin/7/94 (see, e.g., Halperin et al., 1998), influenza virus B/Hong Kong/5/72 (see, e.g., Sidwell et al., 1998), influenza virus B/Lee/40 (see, e.g., Miyamoto et al., 1998), influenza virus B/Victoria group (see, e.g., Nakagawa et al., 1999), influenza virus B/Yamagata 16/88 (see, e.g., Zakay-Rones et al., 1995), influenza virus B/Yamagata group (see, e.g., Nakagawa et al., 1999), influenza virus B/Yamanashi/166/98 (see, e.g., Hoffmann et al., 2002), influenza virus C (see, e.g., Chare et al., 2003), influenza virus strain A/Equi/2/Kildare/89 (see, e.g., Quinlivan et al., 2004), influenza virus type B/Panama 45/90 (see, e.g., Zakay-Rones et al., 1995), live, cold-adapted, temperature-sensitive (ca/ts) Russian influenza A vaccines (see, e.g., Palker et al., 2004), swine H1 and H3 influenza viruses (see, e.g., Gambaryan et al., 2005), swine influenza A viruses (see, e.g., Landolt et al., 2005), swine influenza virus (SIV) (see, e.g., Clavijo et al., 2002), swine influenza virus A/Sw/Ger 2/81 (see, e.g., Zakay-Rones et al., 1995), swine influenza virus A/Sw/Ger 8533/91 (see, e.g., Zakay-Rones et al., 1995), swine influenza virus strain A/Swine/Wisconsin/125/97 (H1N1) (see, e.g., Karasin et al., 2002; Karasin et al., 2006), swine influenza virus strain A/Swine/Wisconsin/136/97 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Wisconsin/163/97 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Wisconsin/164/97 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Wisconsin/166/97 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Wisconsin/168/97 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Wisconsin/235/97 (H1N1) (see, e.g., Karasin et al., 2002; Olsen et al., 2000), swine influenza virus strain A/Swine/Wisconsin/238/97 (H1N1) (see, e.g., Karasin et al., 2002; Ayora-Talayera et al., 2005), swine influenza virus strain A/Swine/Wisconsin/457/98 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Wisconsin/458/98 (H1N1) (see, e.g., Karasin et al., 2002; Karasin et al., 2006), swine influenza virus strain A/Swine/Wisconsin/464/98 (H1N1) (see, e.g., Karasin et al., 2002; Karasin et al., 2006), swine influenza virus strain A/Swine/Indiana/1726/88 (H1N1) (see, e.g., Karasin et al., 2002; Macklin et al., 1998), swine influenza virus strain A/Swine/Indiana/9K035/99 (H1N2) (see, e.g., Karasin et al., 2002; Karasin et al., 2000), swine influenza virus strain A/Swine/Nebraska/1/92 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Quebec/91 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Quebec/81 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/New Jersey/11/76 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Ehime/1/80 (H1N2) (see, e.g., Karasin et al., 2002; Nerome et al., 1985), swine influenza virus strain A/Swine/England/283902/93 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/England/195852/92 (H1N1) (see, e.g., Karasin et al., 2002; Brown et al., 1993), swine influenza virus strain A/Swine/Germany/8533/91 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Germany/2/81 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Nebraska/209/98 (H3N2) (see, e.g., Karasin et al., 2002), A/Swine/Iowa/533/99 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Iowa/569/99 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Minnesota/593/99 (H3N2) (see, e.g., Karasin et al., 2002; Ayora-Talayera et al., 2005), swine influenza virus strain A/Swine/Iowa/8548-1/98 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Minnesota/9088-2/98 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Texas/4199-2/98 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Ontario/41848/97 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/North Carolina/35922/98 (H3N2) (see, e.g., Karasin et al., 2002), /Swine/Colorado/1/77 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Hong Kong/3/76 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Hong Kong/13/77 (H3N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Nagasaki/1/90 (H1N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Nagasaki/1/89 (H1N2) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Wisconsin/1915/88 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Iowa/17672/88 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Tennessee/24/77 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Ontario/2/81 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Wisconsin/1/67 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Italy/1521/98 (H1N2) (see, e.g., Marozin et al., 2002), swine influenza virus strain A/Swine/Italy/839/89 (H1N1) (see, e.g., Karasin et al., 2002), swine influenza virus strain A/Swine/Hong Kong/126/82 (H3N2) (see, e.g., Karasin et al., 2002), influenza virus strain A/Idaho/4/95 (H3N2) (see, e.g., Karasin et al., 2002), influenza virus strain A/Johannesburg/33/94 (H3N2) (see, e.g., Karasin et al., 2002; Johansson et al., 1998), influenza virus strain A/Bangkok/1/79 (H3N2) (see, e.g., Karasin et al., 2002; Nelson et al., 2001), influenza virus strain A/Jdorn/72 (H3N2) (see, e.g., Karasin et al., 2002; Markoff et al., 1982), influenza virus strain A/Hokkaido/2/92 (H1N1) (see, e.g., Karasin et al., 2002), influenza virus strain A/Thailand/KAN-1/04 (see, e.g., Puthavathana et al., 2005; Amonsin et al., 2006), influenza virus strain A/England/1/53 (see, e.g., Govorkova E A, et al., 1995), influenza virus strain A/Vietnam/3046/2004 (H5N1), (see, e.g., Anwar et al., 2006), influenza virus strain A/Vietnam/1203/2004 (H5N1), (see, e.g., Anwar et al., 2006; Gao et al., 2006), influenza virus strain A/tiger/Thailand/SPB-1(H5N1), (see, e.g., Anwar et al., 2006), influenza virus strain A/Japan/305/57 (H2N2) (see, e.g., Naeve et al., 1990; Brown et al., 1982), influenza virus strain A/Adachi/2/57 (H2N2) (see, e.g., Gething et al., 1980), influenza virus strain A/Camel/Mongolia/82 (H1N1) (see, e.g., Yamnikova et al., 1993), influenza virus strain A/R1/5/57 (H2N2) (see, e.g., Elleman et al., 1982), influenza virus strain A/Whale/Maine/1/84 (H13N9) (see, e.g., Air et al., 1987), influenza virus strain A/Taiwan/1/86 (H1N1) (see, e.g., Karasin et al., 2002; Brown, 1988), influenza virus strain A/Bayern/7/95 (H1N1) (see, e.g., Karasin et al., 2002), influenza virus strain A/USSR/90/77 (H1N1) (see, e.g., Karasin et al., 2002; Iftimovici et al., 1980), influenza virus strain A/Wuhan/359/95 (H3N2) (see, e.g., Karasin et al., 2002; Hardy et al., 2001), influenza virus strain A/Hong Kong/5/83 (H3N2) (see, e.g., Karasin et al., 2002), influenza virus strain A/Memphis/8/88 (H3N2) (see, e.g., Karasin et al., 2002; Hatta et al., 2002), influenza virus strain A/Beijing/337/89 (H3N2) (see, e.g., Karasin et al., 2002), influenza virus strain A/Shanghai/6/90 (H3N2) (see, e.g., Karasin et al., 2002), influenza virus strain A/Akita/1/94 (H3N2) (see, e.g., Karasin et al., 2002), influenza virus strain A/Akita/1/95 (H3N2) (see, e.g., Karasin et al., 2002), influenza virus strain A/Memphis/6/90 (H3N2) (see, e.g., Karasin et al., 2002), influenza virus strain AAUdom/307/72 (H3N2) (see, e.g., Karasin et al., 2002; Tuferov et al., 1984), influenza virus strain A/Singapore/1/57 (H2N2) (see, e.g., Karasin et al., 2002; Zhukova et al., 1975), influenza virus strain A/Ohio/4/83 (H1N1) (see, e.g., Karasin et al., 2002), influenza virus strain Madin Darby Canine Kidney (MDCK) -derived cell line (see, e.g., Halperin et al., 2002), mouse-adapted influenza virus strain A/Guizhou/54/89 (H3N2 subtype) (see, e.g., Nagai et al., 1995), mouse-adapted influenza virus A/PR/8/34 (A/PR8) (see, e.g., Nagai et al., 1995), mouse-adapted influenza virus B/Ibaraki/2/85 (see, e.g., Nagai et al., 1995), Russian live attenuated influenza vaccine donor strains A/Leningrad/134/17/57, A/Leningrad/134/47/57 and B/USSR/60/69 (see, e.g., Audsley et al. 2005), the disclosures of which are incorporated by reference. 
     In another advantageous embodiment, the avian influenza polypeptide, antigen, epitope or immunogen may be derived from an avian infected with influenza or an avian influenza strain derived from a recent isolate. 
     In one advantageous embodiment, the influenza vaccine comprises one or more influenza polypeptide, antigen, epitope or immunogen, wherein the influenza isolate is selected from one or more strain of influenza. In one embodiment, the influenza vaccine comprises one or more influenza isolates chosen from the group consisting of a feline influenza isolate, an avian influenza isolate, or mixtures thereof. In yet another embodiment, the influenza vaccine comprises one or more influenza isolates chosen from the group consisting of an inactivated feline influenza isolate, an inactivated avian influenza isolate, or mixtures thereof. 
     As used herein, the term “antigen” or “immunogen” means a substance that induces a specific immune response in a host animal. The antigen may comprise a whole organism, killed, attenuated or live; a subunit or portion of an organism; a recombinant vector containing an insert with immunogenic properties; a piece or fragment of DNA capable of inducing an immune response upon presentation to a host animal; a protein, a polypeptide, a peptide, an epitope, a hapten, or any combination thereof. Alternately, the immunogen or antigen may comprise a toxin or antitoxin. 
     The term “immunogenic protein or peptide” as used herein also refers includes peptides and polypeptides that are immunologically active in the sense that once administered to the host, it is able to evoke an immune response of the humoral and/or cellular type directed against the protein. Preferably the protein fragment is such that it has substantially the same immunological activity as the total protein. Thus, a protein fragment according to the invention comprises or consists essentially of or consists of at least one epitope or antigenic determinant. The term epitope relates to a protein site able to induce an immune reaction of the humoral type (B cells) and/or cellular type (T cells). 
     The term “immunogenic protein or peptide” further contemplates deletions, additions and substitutions to the sequence, so long as the polypeptide functions to produce an immunological response as defined herein. In this regard, particularly preferred substitutions will generally be conservative in nature, i.e., those substitutions that take place within a family of amino acids. For example, amino acids are generally divided into four families: (1) acidic—aspartate and glutamate; (2) basic—lysine, arginine, histidine; (3) non-polar—alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and (4) uncharged polar—glycine, asparagine, glutamine, cystine, serine threonine, tyrosine. Phenylalanine, tryptophan, and tyrosine are sometimes classified as aromatic amino acids. It is reasonably predictable that an isolated replacement of leucine with isoleucine or valine, or vice versa; an aspartate with a glutamate or vice versa; a threonine with a serine or vice versa; or a similar conservative replacement of an amino acid with a structurally related amino acid, will not have a major effect on the biological activity. Proteins having substantially the same amino acid sequence as the reference molecule but possessing minor amino acid substitutions that do not substantially affect the immunogenicity of the protein are, therefore, within the definition of the reference polypeptide. 
     The term “epitope” refers to the site on an antigen or hapten to which specific B cells and/or T cells respond. The term is also used interchangeably with “antigenic determinant” or “antigenic determinant site”. Antibodies that recognize the same epitope can be identified in a simple immunoassay showing the ability of one antibody to block the binding of another antibody to a target antigen. 
     An “immunological response” to a composition or vaccine is the development in the host of a cellular and/or antibody-mediated immune response to a composition or vaccine of interest. Usually, an “immunological response” includes but is not limited to one or more of the following effects: the production of antibodies, B cells, helper T cells, and/or cytotoxic T cells, directed specifically to an antigen or antigens included in the composition or vaccine of interest. Preferably, the host will display either a therapeutic or protective immunological response such that resistance to new infection will be enhanced and/or the clinical severity of the disease reduced. Such protection will be demonstrated by either a reduction or lack of symptoms normally displayed by an infected host, a quicker recovery time and/or a lowered viral titer in the infected host. 
     The terms “immunogenic” protein or polypeptide as used herein also refers to an amino acid sequence which elicits an immunological response as described above. An “immunogenic” protein or polypeptide, as used herein, includes the full-length sequence of the protein, analogs thereof, or immunogenic fragments thereof. By “immunogenic fragment” is meant a fragment of a protein which includes one or more epitopes and thus elicits the immunological response described above. Such fragments can be identified using any number of epitope mapping techniques, well known in the art. See, e.g., Epitope Mapping Protocols in Methods in Molecular Biology, Vol. 66 (Glenn E. Morris, Ed., 1996). For example, linear epitopes may be determined by e.g., concurrently synthesizing large numbers of peptides on solid supports, the peptides corresponding to portions of the protein molecule, and reacting the peptides with antibodies while the peptides are still attached to the supports. Such techniques are known in the art and described in, e.g., U.S. Pat. No. 4,708,871; Geysen et al., 1984; Geysen et al., 1986, all incorporated herein by reference in their entireties. Similarly, conformational epitopes are readily identified by determining spatial conformation of amino acids such as by, e.g., x-ray crystallography and 2-dimensional nuclear magnetic resonance. See, e.g., Epitope Mapping Protocols, supra. Methods especially applicable to the proteins of T. parva are fully described in the PCT Application Serial No. PCT/US2004/022605 incorporated herein by reference in its entirety. 
     Synthetic antigens are also included within the definition, for example, polyepitopes, flanking epitopes, and other recombinant or synthetically derived antigens. See, e.g., Bergmann et al., 1993; Bergmann et al., 1996; Suhrbier, 1997; Gardner et al., 1998. Immunogenic fragments, for purposes of the present invention, will usually include at least about 3 amino acids, preferably at least about 5 amino acids, more preferably at least about 10-15 amino acids, and most preferably about 15-25 amino acids or more amino acids, of the molecule. There is no critical upper limit to the length of the fragment, which could comprise nearly the full-length of the protein sequence, or even a fusion protein comprising at least one epitope of the protein. 
     Accordingly, a minimum structure of a polynucleotide expressing an epitope is that it comprises or consists essentially of or consists of nucleotides to encode an epitope or antigenic determinant of an influenza protein or polyprotein. A polynucleotide encoding a fragment of the total protein or polyprotein, more advantageously, comprises or consists essentially of or consists of a minimum of 15 nucleotides, at least 15-30, advantageously about 30-45 nucleotides, and preferably about 45-75, at least 57, 87 or 150 consecutive or contiguous nucleotides of the sequence encoding the total protein or polyprotein. Epitope determination procedures, such as, generating overlapping peptide libraries (Hemmer et al., 1998), Pepscan (Geysen et al., 1984; Geysen et al., 1985; Van der Zee R. et al., 1989; Geysen, 1990; Multipin.®. Peptide Synthesis Kits de Chiron) and algorithms (De Groot et al., 1999), and in PCT Application Serial No. PCT/US2004/022605 all of which are incorporated herein by reference in their entireties, can be used in the practice of the invention, without undue experimentation. Other documents cited and incorporated herein may also be consulted for methods for determining epitopes of an immunogen or antigen and thus nucleic acid molecules that encode such epitopes. 
     A “polynucleotide” is a polymeric form of nucleotides of any length, which contain deoxyribonucleotides, ribonucleotides, and analogs in any combination. Polynucleotides may have three-dimensional structure, and may perform any function, known or unknown. The term “polynucleotide” includes double-, single-stranded, and triple-helical molecules. Unless otherwise specified or required, any embodiment of the invention described herein that is a polynucleotide encompasses both the double stranded form and each of two complementary forms known or predicted to make up the double stranded form of either the DNA, RNA or hybrid molecule. 
     The following are non-limiting examples of polynucleotides: a gene or gene fragment, exons, introns, mRNA, tRNA, rRNA, ribozymes, cDNA, recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA of any sequence, isolated RNA of any sequence, nucleic acid probes and primers. A polynucleotide may comprise modified nucleotides, such as methylated nucleotides and nucleotide analogs, uracyl, other sugars and linking groups such as fluororibose and thiolate, and nucleotide branches. The sequence of nucleotides may be further modified after polymerization, such as by conjugation, with a labeling component. Other types of modifications included in this definition are caps, substitution of one or more of the naturally occurring nucleotides with an analog, and introduction of means for attaching the polynucleotide to proteins, metal ions, labeling components, other polynucleotides or solid support. The polynucleotides can be obtained by chemical synthesis or derived from a microorganism. 
     The invention further comprises a complementary strand to a polynucleotide encoding an influenza antigen, epitope or immunogen. The complementary strand can be polymeric and of any length, and can contain deoxyribonucleotides, ribonucleotides, and analogs in any combination. 
     The terms “protein”, “peptide”, “polypeptide” and “polypeptide fragment” are used interchangeably herein to refer to polymers of amino acid residues of any length. The polymer can be linear or branched, it may comprise modified amino acids or amino acid analogs, and it may be interrupted by chemical moieties other than amino acids. The terms also encompass an amino acid polymer that has been modified naturally or by intervention; for example disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation or modification, such as conjugation with a labeling or bioactive component. 
     An “isolated” polynucleotide or polypeptide is one that is substantially free of the materials with which it is associated in its native environment. By substantially free, is meant at least 50%, advantageously at least 70%, more advantageously at least 80%, and even more advantageously at least 90% or at least 95% free of these materials. 
     Hybridization reactions can be performed under conditions of different “stringency.” Conditions that increase stringency of a hybridization reaction are well known. See for example, “Molecular Cloning: A Laboratory Manual”, second edition (Sambrook et al., 1989). Examples of relevant conditions include (in order of increasing stringency): incubation temperatures of 25° C., 37° C., 50° C., and 68° C.; buffer concentrations of 10×SSC, 6×SSC, 1×SSC, 0.1×SSC (where SSC is 0.15 M NaCl and 15 mM citrate buffer) and their equivalent using other buffer systems; formamide concentrations of 0%, 25%, 50%, and 75%; incubation times from 5 minutes to 24 hours; 1, 2 or more washing steps; wash incubation times of 1, 2, or 15 minutes; and wash solutions of 6×SSC, 1×SSC, 0.1×SSC, or deionized water. 
     The invention further encompasses polynucleotides encoding functionally equivalent variants and derivatives of the influenza polypeptides and functionally equivalent fragments thereof which may enhance, decrease or not significantly affect properties of the polypeptides encoded thereby. These functionally equivalent variants, derivatives, and fragments display the ability to retain influenza activity. For instance, changes in a DNA sequence that do not change the encoded amino acid sequence, as well as those that result in conservative substitutions of amino acid residues, one or a few amino acid deletions or additions, and substitution of amino acid residues by amino acid analogs are those which will not significantly affect properties of the encoded polypeptide. Conservative amino acid substitutions are glycine/alanine; valine/isoleucine/leucine; asparagine/glutamine; aspartic acid/glutamic acid; serine/threonine/methionine; lysine/arginine; and phenylalanine/tyrosine/tryptophan. In one embodiment, the variants have at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology or identity to the influenza polynucleotide or polypeptide of interest. 
     For the purposes of the present invention, sequence identity or homology is determined by comparing the sequences when aligned so as to maximize overlap and identity while minimizing sequence gaps. In particular, sequence identity may be determined using any of a number of mathematical algorithms. A nonlimiting example of a mathematical algorithm used for comparison of two sequences is the algorithm of Karlin et al., 1990 modified as in Karlin et al., 1993. 
     Another example of a mathematical algorithm used for comparison of sequences is the algorithm of Myers et al., 1988. Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Yet another useful algorithm for identifying regions of local sequence similarity and alignment is the FASTA algorithm as described in Pearson et al., 1988. 
     Advantageous for use according to the present invention is the WU-BLAST (Washington University BLAST) version 2.0 software. WU-BLAST version 2.0 executable programs for several UNIX platforms can be downloaded from ftp://blast.wustl.edu/blast/executables. This program is based on WU-BLAST version 1.4, which in turn is based on the public domain NCBI-BLAST version 1.4 (Altschul et al., 1996; Altschul et al., 1990; Gish et al., 1993; Karlin et al., 1993; all of which are incorporated by reference herein). 
     In general, comparison of amino acid sequences is accomplished by aligning an amino acid sequence of a polypeptide of a known structure with the amino acid sequence of a the polypeptide of unknown structure. Amino acids in the sequences are then compared and groups of amino acids that are homologous are grouped together. This method detects conserved regions of the polypeptides and accounts for amino acid insertions and deletions. Homology between amino acid sequences can be determined by using commercially available algorithms (see also the description of homology above). In addition to those otherwise mentioned herein, mention is made too of the programs BLAST, gapped BLAST, BLASTN, BLASTP, and PSI-BLAST, provided by the National Center for Biotechnology Information. These programs are widely used in the art for this purpose and can align homologous regions of two amino acid sequences. 
     In all search programs in the suite the gapped alignment routines are integral to the database search itself. Gapping can be turned off if desired. The default penalty (Q) for a gap of length one is Q=9 for proteins and BLASTP, and Q=10 for BLASTN, but may be changed to any integer. The default per-residue penalty for extending a gap (R) is R=2 for proteins and BLASTP, and R=10 for BLASTN, but may be changed to any integer. Any combination of values for Q and R can be used in order to align sequences so as to maximize overlap and identity while minimizing sequence gaps. The default amino acid comparison matrix is BLOSUM62, but other amino acid comparison matrices such as PAM can be utilized. 
     Alternatively or additionally, the term “homology” or “identity”, for instance, with respect to a nucleotide or amino acid sequence, can indicate a quantitative measure of homology between two sequences. The percent sequence homology can be calculated as 
     (N ref −N dif )*100/N ref  wherein N dif  is the total number of non-identical residues in the two sequences when aligned and wherein N ref  is the number of residues in one of the sequences. Hence, the DNA sequence AGTCAGTC will have a sequence identity of 75% with the sequence AATCAATC (N ref =8; N dif =2). 
     Alternatively or additionally, “homology” or “identity” with respect to sequences can refer to the number of positions with identical nucleotides or amino acids divided by the number of nucleotides or amino acids in the shorter of the two sequences wherein alignment of the two sequences can be determined in accordance with the Wilbur and Lipman algorithm (Wilbur et al., 1983, incorporated herein by reference), for instance, using a window size of 20 nucleotides, a word length of 4 nucleotides, and a gap penalty of 4, and computer-assisted analysis and interpretation of the sequence data including alignment can be conveniently performed using commercially available programs (e.g., Intelligenetics™ Suite, Intelligenetics Inc. CA). When RNA sequences are said to be similar, or have a degree of sequence identity or homology with DNA sequences, thymidine (T) in the DNA sequence is considered equal to uracil (U) in the RNA sequence. Thus, RNA sequences are within the scope of the invention and can be derived from DNA sequences, by thymidine (T) in the DNA sequence being considered equal to uracil (U) in RNA sequences. 
     And, without undue experimentation, the skilled artisan can consult with many other programs or references for determining percent homology. 
     The invention further encompasses the influenza polynucleotides contained in a vector molecule or an expression vector and operably linked to a promoter element and optionally to an enhancer. 
     The vector is advantageously a poxvirus, particularly a vaccinia virus or an avipox virus, such as fowlpox virus or canarypox virus. Advantageously, the virus is a fowlpox virus. An advantageous fowlpox strain may be an attenuated strain. The vector can express at least one epitope from avian strains. Advantageous fowlpox constructs include, but are not limited to, vFP89 and vFP2211. Alternatively, the virus is advantageously a canarypox virus. Advantageous canarypox strains may be an attenuated strain. The vector can express at least one epitope of an avian strain. An advantageous canarypox construct includes, but is not limited to, vCP2241. Recombinant avipox viruses (see, e.g., U.S. Pat. Nos. 5,505,941 and 5,756,103), such as an attenuated recombinant fowlpox virus, for instance TROVAC (available under ATCC accession number VR-2553), or an attenuated canarypox virus, for instance ALVAC (available under ATCC accession number VR-2547), are especially advantageous. In one advantageous embodiment, the recombinant TROVAC vaccine described by Karaca et al., 2005, the disclosure of which is incorporated by reference, may be used as a feline influenza immunological composition or vaccine. Other viruses that may be used in methods of the invention include, but are not limited to, vaccinia viruses, such as an attenuated vaccinia virus, for instance NYVAC (available under ATCC accession number VR-2259), adenoviruses, such as canine adenoviruses (CAV), and herpesviruses, such as canine herpesvirus (CHV) or a feline herpesvirus (FHV). 
     A “vector” refers to a recombinant DNA or RNA plasmid or virus that comprises a heterologous polynucleotide to be delivered to a target cell, either in vitro or in vivo. The heterologous polynucleotide may comprise a sequence of interest for purposes of prevention or therapy, and may optionally be in the form of an expression cassette. As used herein, a vector needs not be capable of replication in the ultimate target cell or subject. The term includes cloning vectors also included are viral vectors. 
     The term “recombinant” means a polynucleotide semisynthetic, or synthetic origin which either does not occur in nature or is linked to another polynucleotide in an arrangement not found in nature. 
     “Heterologous” means derived from a genetically distinct entity from the rest of the entity to which it is being compared. For example, a polynucleotide, may be placed by genetic engineering techniques into a plasmid or vector derived from a different source, and is a heterologous polynucleotide. A promoter removed from its native coding sequence and operatively linked to a coding sequence other than the native sequence is a heterologous promoter. 
     The polynucleotides of the invention may comprise additional sequences, such as additional encoding sequences within the same transcription unit, controlling elements such as promoters, ribosome binding sites, 5′UTR, 3′UTR, transcription terminators, polyadenylation sites, additional transcription units under control of the same or a different promoter, sequences that permit cloning, expression, homologous recombination, and transformation of a host cell, and any such construct as may be desirable to provide embodiments of this invention. 
     Elements for the expression of an influenza polypeptide, antigen, epitope or immunogen are advantageously present in an inventive vector. In minimum manner, this comprises, consists essentially of, or consists of an initiation codon (ATG), a stop codon and a promoter, and optionally also a polyadenylation sequence for certain vectors such as plasmid and certain viral vectors, e.g., viral vectors other than poxviruses. When the polynucleotide encodes a polyprotein fragment, e.g. an influenza peptide, advantageously, in the vector, an ATG is placed at 5′ of the reading frame and a stop codon is placed at 3′. Other elements for controlling expression may be present, such as enhancer sequences, stabilizing sequences, such as intron and signal sequences permitting the secretion of the protein. 
     Methods for making and/or administering a vector or recombinants or plasmid for expression of gene products of genes of the invention either in vivo or in vitro can be any desired method, e.g., a method which is by or analogous to the methods disclosed in, or disclosed in documents cited in: U.S. Pat. Nos. 4,603,112; 4,769,330; 4,394,448; 4,722,848; 4,745,051; 4,769,331; 4,945,050; 5,494,807; 5,514,375; 5,744,140; 5,744,141; 5,756,103; 5,762,938; 5,766,599; 5,990,091; 5,174,993; 5,505,941; 5,338,683; 5,494,807; 5,591,639; 5,589,466; 5,677,178; 5,591,439; 5,552,143; 5,580,859; 6,130,066; 6,004,777; 6,130,066; 6,497,883; 6,464,984; 6,451,770; 6,391,314; 6,387,376; 6,376,473; 6,368,603; 6,348,196; 6,306,400; 6,228,846; 6,221,362; 6,217,883; 6,207,166; 6,207,165; 6,159,477; 6,153,199; 6,090,393; 6,074,649; 6,045,803; 6,033,670; 6,485,729; 6,103,526; 6,224,882; 6,312,682; 6,348,450, 6,312,683, and 6,596,279; U.S. patent application Ser. No. 920,197, filed Oct. 16, 1986; WO 90/01543; WO91/11525; WO 94/16716; WO 96/39491; WO 98/33510; EP 265785; EP 0 370 573; Andreansky et al., 1996; Ballay et al., 1993; Felgner et al., 1994; Frolov et al., 1996; Graham, 1990; Grunhaus et al., 1992; Ju et al., 1998; Kitson et al., 1991; McClements et al., 1996; Moss, 1996; Paoletti, 1996; Pennock et al., 1984; Richardson (Ed), 1995; Smith et al., 1983; Robertson et al., 1996; Robinson et al., 1997; and Roizman, 1996. Thus, the vector in the invention can be any suitable recombinant virus or virus vector, such as a poxvirus (e.g., vaccinia virus, avipox virus, canarypox virus, fowlpox virus, raccoonpox virus, swinepox virus, etc.), adenovirus (e.g., human adenovirus, canine adenovirus), herpesvirus (e.g. canine herpesvirus), baculovirus, retrovirus, etc. (as in documents incorporated herein by reference); or the vector can be a plasmid. The herein cited and incorporated herein by reference documents, in addition to providing examples of vectors useful in the practice of the invention, can also provide sources for non-influenza peptides or fragments thereof to be expressed by vector or vectors in, or included in, the compositions of the invention. 
     The present invention also relates to preparations comprising vectors, such as expression vectors, e.g., therapeutic compositions. The preparations can comprise, consist essentially of, or consist of one or more vectors, e.g., expression vectors, such as in vivo expression vectors, comprising, consisting essentially or consisting of (and advantageously expressing) one or more of influenza polypeptides, antigens, epitopes or immunogens. Advantageously, the vector contains and expresses a polynucleotide that includes, consists essentially of, or consists of a polynucleotide coding for (and advantageously expressing) an influenza antigen, epitope or immunogen, in a pharmaceutically or veterinarily acceptable carrier, excipient or vehicle. Thus, according to an embodiment of the invention, the other vector or vectors in the preparation comprises, consists essentially of or consists of a polynucleotide that encodes, and under appropriate circumstances the vector expresses one or more other proteins of an influenza polypeptide, antigen, epitope or immunogen (e.g., hemagglutinin, neuraminidase, nucleoprotein) or a fragment thereof. 
     According to another embodiment, the vector or vectors in the preparation comprise, or consist essentially of, or consist of polynucleotide(s) encoding one or more proteins or fragment(s) thereof of an influenza polypeptide, antigen, epitope or immunogen, the vector or vectors expressing the polynucleotide(s). The inventive preparation advantageously comprises, consists essentially of, or consists of, at least two vectors comprising, consisting essentially of, or consisting of, and advantageously also expressing, advantageously in vivo under appropriate conditions or suitable conditions or in a suitable host cell, polynucleotides from different feline influenza isolates encoding the same proteins and/or for different proteins, but advantageously the same proteins. Preparations containing one or more vectors containing, consisting essentially of or consisting of polynucleotides encoding, and advantageously expressing, advantageously in vivo, an influenza polypeptide, antigen, fusion protein or an epitope thereof. The invention is also directed at mixtures of vectors that contain, consist essentially of, or consist of coding for, and express, different influenza polypeptides, antigens, epitopes or immunogens, e.g., an influenza polypeptide, antigen, epitope or immunogen from different species such as, but not limited to, humans, horses, pigs, in addition to avian species including chicken, ducks and geese. 
     According to one embodiment of the invention, the expression vector is a viral vector, in particular an in vivo expression vector. In an advantageous embodiment, the expression vector is an adenovirus vector. Advantageously, the adenovirus is a human Ad5 vector, an E1-deleted and/or an E3-deleted adenovirus. 
     In one particular embodiment the viral vector is a poxvirus, e.g. a vaccinia virus or an attenuated vaccinia virus, (for instance, MVA, a modified Ankara strain obtained after more than 570 passages of the Ankara vaccine strain on chicken embryo fibroblasts; see Stickl et al., 1971; Sutter et al., 1992; available as ATCC VR-1508; or NYVAC, available under VR-2259, see U.S. Pat. No. 5,494,807, for instance, Examples 1 to 6 and et seq of U.S. Pat. No. 5,494,807 which discuss the construction of NYVAC, as well as variations of NYVAC with additional ORFs deleted from the Copenhagen strain vaccinia virus genome, as well as the insertion of heterologous coding nucleic acid molecules into sites of this recombinant, and also, the use of matched promoters; see also WO96/40241), an avipox virus or an attenuated avipox virus (e.g., canarypox, fowlpox, dovepox, pigeonpox, quailpox, ALVAC, available under VR-2547, or TROVAC; available under VR-2553, see, e.g., U.S. Pat. Nos. 5,505,941, 5,494,807), swinepox, raccoonpox, camelpox, or myxomatosis virus. 
     According to another embodiment of the invention, the poxvirus vector is a fowlpox virus or a canarypox virus vector, advantageously an attenuated fowlpox virus or canarypox virus. In this regard, attenuated fowlpox viruses are available, such asDIFTOSEC CT strain marketed by MERIAL and the NOBILIS VARIOLE vaccine marketed by INTERVET. Attenuated fowlpox viruses are described in U.S. Pat. No. 5,766,599 which pertains to the attenuated fowlpox strain TROVAC. Canarypox viruses are also available from the ATCC under access number VR-111, and are described in U.S. Pat. No. 5,756,103 (ALVAC) and WO01/05934. 
     For information on the method to generate recombinants thereof and how to administer recombinants thereof, the skilled artisan can refer documents cited herein and to WO90/12882, e.g., as to vaccinia virus mention is made of U.S. Pat. Nos. 4,769,330, 4,722,848, 4,603,112, 5,110,587, 5,494,807, and 5,762,938 inter alia; as to fowlpox, mention is made of U.S. Pat. Nos. 5,174,993, 5,505,941 and U.S. Pat. No. 5,766,599 inter alia; as to canarypox mentionis made of U.S. Pat. No. 5,756,103 inter alia; as to swinepox mention is made of U.S. Pat. No. 5,382,425 inter alia; and, as to raccoonpox, mention is made of WO00/03030 inter alia. 
     When the expression vector is a vaccinia virus, insertion site or sites for the polynucleotide or polynucleotides to be expressed are advantageously at the thymidine kinase (TK) gene or insertion site, the hemagglutinin (HA) gene or insertion site, the region encoding the inclusion body of the A type (ATI); see also documents cited herein, especially those pertaining to vaccinia virus. In the case of fowlpox, advantageously the insertion site or sites are ORFs F7 and/or F8; see also documents cited herein, especially those pertaining to fowlpox virus. In the case of canarypox, advantageously the insertion site or sites are ORF(s) C3, C5 and/or C6; see also documents cited herein, especially those pertaining to canarypox virus. The insertion site or sites for MVA virus area advantageously as in various publications, including Carroll et al., 1997; Stittelaar et al., 2000; Sutter et al., 1994; and, in this regard it is also noted that the complete MVA genome is described in Antoine, 1998, which enables the skilled artisan to use other insertion sites or other promoters. 
     Advantageously, the polynucleotide to be expressed is inserted under the control of a specific poxvirus promoter, e.g., the vaccinia promoter 7.5 kDa (Cochran et al., 1985), the vaccinia promoter 13L (Riviere et al., 1992), the vaccinia promoter HA (Shida, 1986), the cowpox promoter ATI (Funahashi et al., 1988), the vaccinia promoter H6 (Taylor et al., 1988b; Guo et al., 1989; Perkus et al., 1989), inter alia. 
     In a particular embodiment the viral vector is an adenovirus, such as a human adenovirus (HAV) or a canine adenovirus (CAV). 
     In one embodiment the viral vector is a human adenovirus, in particular a serotype 5 adenovirus, rendered incompetent for replication by a deletion in the E1 region of the viral genome, in particular from about nucleotide 459 to about nucleotide 3510 by reference to the sequence of the hAd5 disclosed in Genbank under the accession number M73260 and in the referenced publication Chroboczek et al, 1992. The deleted adenovirus is propagated in E1-expressing 293 (Graham et al., 1977) or PER cells, in particular PER.C6 (Falloux et al., 1998). The human adenovirus can be deleted in the E3 region, in particular from about nucleotide 28592 to about nucleotide 30470. The deletion in the E1 region can be done in combination with a deletion in the E3 region (see, e.g. Shriver et al., 2002; Graham et al., 1991; Ilan et al., 1997; U.S. Pat. Nos. 6,133,028 and 6,692,956; Tripathy et al., 1994; Tapnell, 1993; Danthinne et al., 2000; Berkner, 1988; Berkner et al., 1983; Chavier et al., 1996). The insertion sites can be the E1 and/or E3 loci (region) eventually after a partial or complete deletion of the E1 and/or E3 regions. Advantageously, when the expression vector is an adenovirus, the polynucleotide to be expressed is inserted under the control of a promoter functional in eukaryotic cells, such as a strong promoter, preferably a cytomegalovirus immediate-early gene promoter (CMV-IE promoter), in particular the enhancer/promoter region from about nucleotide −734 to about nucleotide +7 in Boshart et al., 1985 or the enhancer/promoter region from the pCI vector from Promega Corp. The CMV-IE promoter is advantageously of murine or human origin. The promoter of the elongation factor 1 a can also be used. A muscle specific promoter can also be used (Li et al., 1999). Strong promoters are also discussed herein in relation to plasmid vectors. In one embodiment, a splicing sequence can be located downstream of the enhancer/promoter region. For example, the intron 1 isolated from the CMV-IE gene (Stenberg et al., 1984), the intron isolated from the rabbit or human β-globin gene, in particular the intron 2 from the b-globin gene, the intron isolated from the immunoglobulin gene, a splicing sequence from the SV40 early gene or the chimeric intron sequence isolated from the pCI vector from Promege Corp. comprising the human β-globin gene donor sequence fused to the mouse immunoglobulin acceptor sequence (from about nucleotide 890 to about nucleotide 1022 in Genbank under the accession number CVU47120). A poly(A) sequence and terminator sequence can be inserted downstream the polynucleotide to be expressed, e.g. a bovine growth hormone gene, in particular from about nucleotide 2339 to about nucleotide 2550 in Genbank under the accession number BOVGHRH, a rabbit β-globin gene or a SV40 late gene polyadenylation signal. 
     In another embodiment the viral vector is a canine adenovirus, in particular a CAV-2 (see, e.g. Fischer et al., 2002; U.S. Pat. Nos. 5,529,780 and 5,688,920; PCT Application No. WO95/14102). For CAV, the insertion sites can be in the E3 region and/or in the region located between the E4 region and the right ITR region (see U.S. Pat. Nos. 6,090,393 and 6,156,567). In one embodiment the insert is under the control of a promoter, such as a cytomegalovirus immediate-early gene promoter (CMV-IE promoter) or a promoter already described for a human adenovirus vector. A poly(A) sequence and terminator sequence can be inserted downstream the polynucleotide to be expressed, e.g. a bovine growth hormone gene or a rabbit β-globin gene polyadenylation signal. 
     In another particular embodiment the viral vector is a herpesvirus such as a canine herpesvirus (CHV) or a feline herpesvirus (FHV). For CHV, the insertion sites may be in particular in the thymidine kinase gene, in the ORF3, or in the UL43 ORF (see U.S. Pat. No. 6,159,477). In one embodiment the polynucleotide to be expressed is inserted under the control of a promoter functional in eukaryotic cells, advantageously a CMV-IE promoter (murine or human). A poly(A) sequence and terminator sequence can be inserted downstream the polynucleotide to be expressed, e.g. bovine growth hormone or a rabbit β-globin gene polyadenylation signal. 
     According to a yet further embodiment of the invention, the expression vector is a plasmid vector or a DNA plasmid vector, in particular an in vivo expression vector. In a specific, non-limiting example, the pVR1020 or 1012 plasmid (VICAL Inc.; Luke et al., 1997; Hartikka et al., 1996, see, e.g., U.S. Pat. Nos. 5,846,946 and 6,451,769) can be utilized as a vector for the insertion of a polynucleotide sequence. The pVR1020 plasmid is derived from pVR1012 and contains the human tPA signal sequence. In one embodiment the human tPA signal comprises from amino acid M(1) to amino acid S(23) in Genbank under the accession number HUMTPA14. In another specific, non-limiting example, the plasmid utilized as a vector for the insertion of a polynucleotide sequence can contain the signal peptide sequence of equine IGF1 from amino acid M(24) to amino acid A(48) in Genbank under the accession number U28070. Additional information on DNA plasmids which may be consulted or employed in the practice are found, for example, in U.S. Pat. Nos. 6,852,705; 6,818,628; 6,586,412; 6,576,243; 6,558,674; 6,464,984; 6,451,770; 6,376,473 and 6,221,362. 
     The term plasmid covers any DNA transcription unit comprising a polynucleotide according to the invention and the elements necessary for its in vivo expression in a cell or cells of the desired host or target; and, in this regard, it is noted that a supercoiled or non-supercoiled, circular plasmid, as well as a linear form, are intended to be within the scope of the invention. 
     Each plasmid comprises or contains or consists essentially of, in addition to the polynucleotide encoding an influenza antigen, epitope or immunogen, optionally fused with a heterologous peptide sequence, variant, analog or fragment, operably linked to a promoter or under the control of a promoter or dependent upon a promoter. In general, it is advantageous to employ a strong promoter functional in eukaryotic cells. The preferred strong promoter is the immediate early cytomegalovirus promoter (CMV-IE) of human or murine origin, or optionally having another origin such as the rat or guinea pig. The CMV-IE promoter can comprise the actual promoter part, which may or may not be associated with the enhancer part. Reference can be made to EP-A-260 148, EP-A-323 597, U.S. Pat. Nos. 5,168,062, 5,385,839, and 4,968,615, as well as to PCT Application No WO87/03905. The CMV-IE promoter is advantageously a human CMV-IE (Boshart et al., 1985) or murine CMV-IE. 
     In more general terms, the promoter has either a viral or a cellular origin. A strong viral promoter other than CMV-IE that may be usefully employed in the practice of the invention is the early/late promoter of the SV40 virus or the LTR promoter of the Rous sarcoma virus. A strong cellular promoter that may be usefully employed in the practice of the invention is the promoter of a gene of the cytoskeleton, such as e.g. the desmin promoter (Kwissa et al., 2000), or the actin promoter (Miyazaki et al., 1989). 
     Functional sub fragments of these promoters, i.e., portions of these promoters that maintain an adequate promoting activity, are included within the present invention, e.g. truncated CMV-IE promoters according to PCT Application No. WO98/00166 or U.S. Pat. No. 6,156,567 can be used in the practice of the invention. A promoter in the practice of the invention consequently includes derivatives and sub fragments of a full-length promoter that maintain an adequate promoting activity and hence function as a promoter, preferably promoting activity substantially similar to that of the actual or full-length promoter from which the derivative or sub fragment is derived, e.g., akin to the activity of the truncated CMV-IE promoters of U.S. Pat. No. 6,156,567 to the activity of full-length CMV-IE promoters. Thus, a CMV-IE promoter in the practice of the invention can comprise or consist essentially of or consist of the promoter portion of the full-length promoter and/or the enhancer portion of the full-length promoter, as well as derivatives and sub fragments. 
     Preferably, the plasmids comprise or consist essentially of other expression control elements. It is particularly advantageous to incorporate stabilizing sequence(s), e.g., intron sequence(s), preferably the first intron of the hCMV-IE (PCT Application No. WO89/01036), the intron II of the rabbit β-globin gene (van Ooyen et al., 1979). 
     As to the polyadenylation signal (polyA) for the plasmids and viral vectors other than poxviruses, use can more be made of the poly(A) signal of the bovine growth hormone (bGH) gene (see U.S. Pat. No. 5,122,458), or the poly(A) signal of the rabbit β-globin gene or the poly(A) signal of the SV40 virus. 
     According to another embodiment of the invention, the expression vectors are expression vectors used for the in vitro expression of proteins in an appropriate cell system. The expressed proteins can be harvested in or from the culture supernatant after, or not after secretion (if there is no secretion a cell lysis typically occurs or is performed), optionally concentrated by concentration methods such as ultrafiltration and/or purified by purification means, such as affinity, ion exchange or gel filtration-type chromatography methods. 
     A “host cell” denotes a prokaryotic or eukaryotic cell that has been genetically altered, or is capable of being genetically altered by administration of an exogenous polynucleotide, such as a recombinant plasmid or vector. When referring to genetically altered cells, the term refers both to the originally altered cell and to the progeny thereof. Advantageous host cells include, but are not limited to, baby hamster kidney (BHK) cells, colon carcinoma (Caco-2) cells, COS7 cells, MCF-7 cells, MCF-10A cells, Madin-Darby canine kidney (MDCK) lines, mink lung (Mv1Lu) cells, MRC-5 cells, U937 cells, CHO cells, and VERO cells. Polynucleotides comprising a desired sequence can be inserted into a suitable cloning or expression vector, and the vector in turn can be introduced into a suitable host cell for replication and amplification. Polynucleotides can be introduced into host cells by any means known in the art. The vectors containing the polynucleotides of interest can be introduced into the host cell by any of a number of appropriate means, including direct uptake, endocytosis, transfection, f-mating, electroporation, transfection employing calcium chloride, rubidium chloride, calcium phosphate, DEAE-dextran, or other substances; microprojectile bombardment; lipofection; and infection (where the vector is infectious, for instance, a retroviral vector). The choice of introducing vectors or polynucleotides will often depend on features of the host cell. 
     In an advantageous embodiment, the invention provides for the administration of a therapeutically effective amount of a formulation for the delivery and expression of an influenza antigen, epitope or immunogen in a target cell. Determination of the therapeutically effective amount is routine experimentation for one of ordinary skill in the art. In one embodiment, the formulation comprises an expression vector comprising a polynucleotide that expresses an influenza antigen, epitope or immunogen and a pharmaceutically or veterinarily acceptable carrier, vehicle or excipient. In an advantageous embodiment, the pharmaceutically or veterinarily acceptable carrier, vehicle or excipient facilitates transfection and/or improves preservation of the vector or protein. 
     The pharmaceutically or veterinarily acceptable carriers or vehicles or excipients are well known to the one skilled in the art. For example, a pharmaceutically or veterinarily acceptable carrier or vehicle or excipient can be a 0.9% NaCl (e.g., saline) solution or a phosphate buffer. Other pharmaceutically or veterinarily acceptable carrier or vehicle or excipients that can be used for methods of this invention include, but are not limited to, poly-(L-glutamate) or polyvinylpyrrolidone. The pharmaceutically or veterinarily acceptable carrier or vehicle or excipients may be any compound or combination of compounds facilitating the administration of the vector (or protein expressed from an inventive vector in vitro); advantageously, the carrier, vehicle or excipient may facilitate transfection and/or improve preservation of the vector (or protein). Doses and dose volumes are herein discussed in the general description and can also be determined by the skilled artisan from this disclosure read in conjunction with the knowledge in the art, without any undue experimentation. 
     The cationic lipids containing a quaternary ammonium salt which are advantageously but not exclusively suitable for plasmids, are advantageously those having the following formula: 
                         
in which R1 is a saturated or unsaturated straight-chain aliphatic radical having 12 to 18 carbon atoms, R2 is another aliphatic radical containing 2 or 3 carbon atoms and X is an amine or hydroxyl group, e.g. the DMRIE. In another embodiment the cationic lipid can be associated with a neutral lipid, e.g. the DOPE.
 
     Among these cationic lipids, preference is given to DMRIE (N-(2-hydroxyethyl)-N,N -dimethyl-2,3-bis(tetradecyloxy)-1-propane ammonium; WO96/34109), advantageously associated with a neutral lipid, advantageously DOPE (dioleoyl-phosphatidyl-ethanol amine; Behr, 1994), to form DMRIE-DOPE. 
     Advantageously, the plasmid mixture with the adjuvant is formed extemporaneously and advantageously contemporaneously with administration of the preparation or shortly before administration of the preparation; for instance, shortly before or prior to administration, the plasmid-adjuvant mixture is formed, advantageously so as to give enough time prior to administration for the mixture to form a complex, e.g. between about 10 and about 60 minutes prior to administration, such as approximately 30 minutes prior to administration. 
     When DOPE is present, the DMRIE:DOPE molar ratio is advantageously about 95: about 5 to about 5: about 95, more advantageously about 1: about 1, e.g., 1:1. 
     The DMRIE or DMRIE-DOPE adjuvant:plasmid weight ratio can be between about 50: about 1 and about 1: about 10, such as about 10: about 1 and about 1 about 5, and advantageously about 1: about 1 and about 1: about 2, e.g., 1:1 and 1:2. 
     The invention also provides for inactivated feline influenza immunological compositions or vaccines. As used herein, the term “inactivated immunological composition” or “inactivated vaccine” means a immunological composition or vaccine containing an infectious organism or pathogen that is no longer capable of replication or growth. Inactivation may be accomplished by a variety of methods sufficient to prevent replication or growth of the organism while maintaining its immunogenicity. 
     The inactivated immunological composition or vaccine may be an inactivated form of an isolate of an influenza virus from an affected cat. The virus may be isolated from the alveoli or lung of an affected cat. In another embodiment, the inactivated immunological composition or vaccine may be an inactivated avian influenza. The inactivated immunological composition or vaccine may be an inactivated version of any one of the influenza strains described above. 
     An inactivated immunological composition or vaccine may be prepared as well from the harvested culture fluid. The virus may be produced either by inoculation of 10-11-day embryonated eggs (U.S. Pat. No. 6,048,537) or by inoculation of BHK-21 cell culture (Ross et al., 1970; Tolstova et al., 1966; Merten et al., 1996), of MDCK cell culture (Tree et al., 2001; Ghendon et al., 2005; Brands et al., 1999; Youil et al., 2004), of Vero cell culture (Kistner et al., 1998; Govorkova et al., 1996). The allantoic fluid or the cell culture supernatant can be clarified by low centrifugation and/or filtration. The virus can be concentrated by ultrafiltration and can be purified by zonal centrifugation on sucrose gradient (U.S. Pat. No. 6,048,537; O. Kistner et al. idem), by gel filtration (Nayak et al., 2005; Tomita et al., 1971). 
     Inactivation may be achieved by treating the viruses by any of the methods commonly employed to make inactivated immunological compositions or vaccines. These methods include but are not limited to formaldehyde treatment (O. Kistner et al. idem; Garcia et al., 1998), betapropriolactone treatment (Budowsky et al., 1991; Budowsky et al., 1993; Keverin et al., 2000), ethylene-imine treatment (Swayne et al., 2001), treatment with organic solvents, treatment with detergents, treatment with Tween-ether or treatment with Triton X-100 (J. Vilay et al. idem) for allantoic fluid. For the inactivation the concentration can be about 0.01-0.2% w/v for the formaldehyde; about 0.03-0.2% w/v for the betapropiolactone; about 0.5-20 mM for ethyleneimine. The methods recited herein serve as art-known examples for inactivating virus. Inactivated virus immunological compositions or vaccines are usually administered mixed with an adjuvant. The inactivated immunological composition or vaccine can be administered to the animal by any of a plurality of methods which include but are not limited to inoculation intramuscularly or subcutaneously, spraying, ocularly, nasally, orally, or in ovo. 
     The immunological compositions and vaccines according to the invention may comprise or consist essentially of one or more adjuvants. Suitable adjuvants for use in the practice of the present invention are (1) polymers of acrylic or methacrylic acid, maleic anhydride and alkenyl derivative polymers, (2) immunostimulating sequences (ISS), such as oligodeoxyribonucleotide sequences having one ore more non-methylated CpG units (Klinman et al., 1996; WO98/16247), (3) an oil in water emulsion, such as the SPT emulsion described on p 147 of “Vaccine Design, The Subunit and Adjuvant Approach” published by M. Powell, M. Newman, Plenum Press 1995, and the emulsion MF59 described on p 183 of the same work, (4) cation lipids containing a quaternary ammonium salt, e.g., DDA (5) cytokines, (6) aluminum hydroxide or aluminum phosphate, (7) saponin or (8) other adjuvants discussed in any document cited and incorporated by reference into the instant application, or (9) any combinations or mixtures thereof. 
     The oil in water emulsion (3), which is especially appropriate for viral vectors, can be based on: light liquid paraffin oil (European pharmacopoeia type), isoprenoid oil such as squalane, squalene, oil resulting from the oligomerization of alkenes, e.g. isobutene or decene, esters of acids or alcohols having a straight-chain alkyl group, such as vegetable oils, ethyl oleate, propylene glycol, di(caprylate/caprate), glycerol tri(caprylate/caprate) and propylene glycol dioleate, or esters of branched, fatty alcohols or acids, especially isostearic acid esters. 
     The oil is used in combination with emulsifiers to form an emulsion. The emulsifiers may be nonionic surfactants, such as: esters of on the one hand sorbitan, mannide (e.g. anhydromannitol oleate), glycerol, polyglycerol or propylene glycol and on the other hand oleic, isostearic, ricinoleic or hydroxystearic acids, said esters being optionally ethoxylated, or polyoxypropylene-polyoxyethylene copolymer blocks, such as Pluronic, e.g., L121. 
     Among the type (1) adjuvant polymers, preference is given to polymers of crosslinked acrylic or methacrylic acid, especially crosslinked by polyalkenyl ethers of sugars or polyalcohols. These compounds are known under the name carbomer (Pharmeuropa, vol. 8, no. 2, June 1996). One skilled in the art can also refer to U.S. Pat. No. 2,909,462, which provides such acrylic polymers crosslinked by a polyhydroxyl compound having at least three hydroxyl groups, preferably no more than eight such groups, the hydrogen atoms of at least three hydroxyl groups being replaced by unsaturated, aliphatic radicals having at least two carbon atoms. The preferred radicals are those containing 2 to 4 carbon atoms, e.g. vinyls, allyls and other ethylenically unsaturated groups. The unsaturated radicals can also contain other substituents, such as methyl. Products sold under the name Carbopol (BF Goodrich, Ohio, USA) are especially suitable. They are crosslinked by allyl saccharose or by allyl pentaerythritol. Among them, reference is made to Carbopol 974P, 934P and 971P. 
     As to the maleic anhydride-alkenyl derivative copolymers, preference is given to EMA (Monsanto), which are straight-chain or crosslinked ethylene-maleic anhydride copolymers and they are, for example, crosslinked by divinyl ether. Reference is also made to J. Fields et al., 1960. 
     With regard to structure, the acrylic or methacrylic acid polymers and EMA are preferably formed by basic units having the following formula: 
                         
in which:
         R1 and R2, which can be the same or different, represent H or CH3   x=0 or 1, preferably x=1   y=1 or 2, with x+y=2.       

     For EMA, x=0 and y=2 and for carbomers x=y=1. 
     These polymers are soluble in water or physiological salt solution (20 g/l NaCl) and the pH can be adjusted to 7.3 to 7.4, e.g., by soda (NaOH), to provide the adjuvant solution in which the expression vector(s) can be incorporated. The polymer concentration in the final immunological or vaccine composition can range between 0.01 and 1.5% w/v, advantageously 0.05 to 1% w/v and preferably 0.1 to 0.4% w/v. 
     The cytokine or cytokines (5) can be in protein form in the immunological or vaccine composition, or can be co-expressed in the host with the immunogen or immunogens or epitope(s) thereof. Preference is given to the co-expression of the cytokine or cytokines, either by the same vector as that expressing the immunogen or immunogens or epitope(s) thereof, or by a separate vector therefor. 
     The invention comprehends preparing such combination compositions; for instance by admixing the active components, advantageously together and with an adjuvant, carrier, cytokine, and/or diluent. 
     Cytokines that may be used in the present invention include, but are not limited to, granulocyte colony stimulating factor (G-CSF), granulocyte/macrophage colony stimulating factor (GM-CSF), interferon α(IFNα), interferon β(IFNβ), interferon γ, (IFNγ), interleukin-1α(IL-1α), interleukin-1β(IL-1β), interleukin-2 (IL-2), interleukin-3 (IL-3), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-6 (IL-6), interleukin-7 (IL-7), interleukin-8 (IL-8), interleukin-9 (IL-9), interleukin-10 (IL-10), interleukin-11 (IL-11), interleukin-12 (IL-12), tumor necrosis factor α(TNFα), tumor necrosis factor β(TNFβ), and transforming growth factor β(TGFβ). It is understood that cytokines can be co-administered and/or sequentially administered with the immunological or vaccine composition of the present invention. Thus, for instance, the vaccine of the instant invention can also contain an exogenous nucleic acid molecule that expresses in vivo a suitable cytokine, e.g., a cytokine matched to this host to be vaccinated or in which an immunological response is to be elicited (for instance, a feline cytokine for preparations to be administered to felids). 
     Advantageously, the immunological composition and/or vaccine according to the invention comprise or consist essentially of or consist of an effective quantity to elicit a therapeutic response of one or more expression vectors and/or polypeptides as discussed herein; and, an effective quantity can be determined from this disclosure, including the documents incorporated herein, and the knowledge in the art, without undue experimentation. 
     In the case of immunological composition and/or vaccine based on a plasmid vector, a dose can comprise, consist essentially of or consist of, in general terms, about in 1 μg to about 2000 μg. advantageously about 50 μg to about 1000 μg and more advantageously from about 100 μg to about 800 μg of plasmid expressing the influenza antigen, epitope or immunogen. When immunological composition and/or vaccine based on a plasmid vector is administered with electroporation the dose of plasmid is generally between about 0.1 μg and 1 mg, advantageously between about 1 μg and 100 μg, advantageously between about 2 μg and 50 μg. The dose volumes can be between about 0.1 and about 2 ml, advantageously between about 0.2 and about 1 ml. These doses and dose volumes are suitable for the treatment of felines. 
     The immunological composition and/or vaccine contains per dose from about 10 4  to about 10 11 , advantageously from about 10 5  to about 10 10  and more advantageously from about 10 6  to about 10 9  viral particles of recombinant adenovirus expressing an influenza antigen, epitope or immunogen. In the case of immunological composition and/or vaccine based on a poxvirus, a dose can be between about 10 2  pfu and about 10 9  pfu. The immunological composition and/or vaccine contains per dose from about 10 5  to 10 9 , advantageously from about 10 6  to 10 8  pfu of poxvirus or herpesvirus recombinant expressing the influenza antigen, epitope or immunogen. 
     The dose volume of compositions for target species that are mammals, e.g., the dose volume of feline compositions, based on viral vectors, e.g., non-poxvirus-viral-vector-based compositions, is generally between about 0.1 to about 2.0 ml, preferably between about 0.1 to about 1.0 ml, and more preferably between about 0.5 ml to about 1.0 ml. 
     In one embodiment of the invention, a prime-boost regimen can be employed, which is comprised of at least one primary administration and at least one booster administration using at least one common polypeptide, antigen, epitope or immunogen. The immunological composition or vaccine used in primary administration is different in nature from those used as a booster. This administration protocol is called “prime-boost”. The prime-boost protocol according to the invention comprises a primary administration with an immunological composition or vaccine comprising, in a pharmaceutically acceptable vehicle or excipient, a plasmid containing a polynucleotide sequence for expressing, in vivo, an avian influenza polypeptide, antigen, epitope or immunogen, followed by a booster with an immunogical composition or vaccine, or a recombined immunological composition or vaccine comprising, in a pharmaceutically acceptable vehicle or excipient, a viral vector containing a polynucleotide sequence for expressing, in vivo, an avian influenza polypeptide antigen, epitope or immunogen, with the condition according to which at least one of the polypeptides, antigens, epitopes or immunogens is encoded by both the plasmids and the viral vectors. Alternatively, the booster can be comprised of an inactivated immunological composition or vaccine. In another alternative embodiment, the avian influenza polypeptide antigen, epitope or immunogen of the primary administration may derive from a different avian influenza strain than the avian influenza polypeptide antigen, epitope or immunogen of the booster administration 
     The primary administration may comprise one or more administrations of the same plasmid-based immunological compositions of vaccines. Similarly, the booster administration may comprise one or more administrations of the same viral vector-based immunological composition of vaccine. According to a particular embodiment of the invention, the protocol comprises two successive administrations of the same plasmid-based immunological compositions of vaccines, and then one administration of a viral vector-based immunological composition of vaccine, as a booster. Alternatively, the primary administration may be a plasmid-based or viral vector-based immunological composition or vaccine, and the booster administration may be an inactivated immunological composition or vaccine. 
     The various administrations are preferably carried out 3 to 6 weeks apart, and more particularly about 4 weeks apart. According to a preferred mode, an annual booster, preferably using the viral vector-based immunological composition of vaccine, is also envisaged. The animals are preferably at least 6 to 8 weeks old at the time of the first administration. 
     With inactivated compositions of the virus or organism or pathogen produced on the new cell culture, the animal may be administered approximately 10 4 -10 9  equivalent CCID50 (titer before inactivation), advantageously approximately 10 5 -10 8  equivalent CCID50 in a single dosage unit. The volume of one single dosage unit can be between 0.2 ml and 5.0 ml and advantageously between 0.5 ml and 2.0 ml and more advantageously about 2.0 ml. One or more administrations can be done; e.g. with two injections at 2-4 weeks interval, and advantageously with a boost about 3 weeks after the first injection. 
     In an advantageous embodiment, an animal, advantageously a felid, is vaccinated with two doses of inactivated vaccine at about 3 to 4 week intervals via the subcuntaneous route, although an intramuscular route is also contemplated. Blood samples may be collected on the day of the first and/or second vaccination and about 2 to 10 weeks after the second vaccination to determine the levels of anti-influenza virus-specific antibodies by methods known to one of skill in the art, for example, virus neutralization, hemagglutination inhibition, ELISA, or single radial heamolysis (SRH) tests. 
     The efficacy of the inactivated vaccines may be tested about 2 to 4 weeks after the second immunization by challenging animals, advantageously felids, with a virulent strain of influenza, advantageously the influenza H5N1, H5N8 or H5N9 strains. The animal may be challenged by spray, intra-nassaly, intra-tracheally, orally, and/or by contact. The challenge viral may be about 10 5-8  EID50 in a volume depending upon the route of administration. For example, if the administration is by spray, a virus suspension is aerosolized to generate about 1 to 100 μm droplets, if the administration is intra-nasal, intra-tracheal or oral, the volume of the challenge virus is about 0.5 ml, 1-2 ml, and 5-10 ml, respectively. Animals may be observed daily for 14 days following challenge for clinical signs, for example, fever, cough, nasal, ocular discharge, respiratory distress, anorexia, and lethargy. In addition, the groups of animals may be euthanized and evaluated for pathological findings of pulmonary and pleural hemorrhage, tracheitis, bronchitis, broncolilitis, and bronchopneumonia. Tracheal swabs may be collected from all animals post challenge days 1-14 for virus isolation. The presence or absence of viral antigens in respiratory tissues may be evaluated by immunohistochemistry, for example, on days 3, 7, and 10 post-challenge. Blood samples may be collected post-challenge (e.g., on days 7 and 14 post-challenge) and may be analyzed for the presence of anti-influenza H5N1 virus-specific antibody. 
     It should be understood by one of skill in the art that the disclosure herein is provided by way of example and the present invention is not limited thereto. From the disclosure herein and the knowledge in the art, the skilled artisan can determine the number of administrations, the administration route, and the doses to be used for each injection protocol, without any undue experimentation. 
     The present invention contemplates at least one administration to an animal of an efficient amount of the therapeutic composition made according to the invention. The animal may be male, female, pregnant female and newborn. This administration may be via various routes including, but not limited to, intramuscular (IM), intradermal (ID) or subcutaneous (SC) injection or via intranasal or oral administration. The therapeutic composition according to the invention can also be administered by a needleless apparatus (as, for example with a Pigjet, Dermojet, Biojector, Vetjet or Vitajet apparatus (Bioject, Oregon, USA)). Another approach to administer plasmid compositions is to use electroporation (see, e.g. Tollefsen et al., 2002; Tollefsen et al., 2003; Babiuk et al., 2002; PCT Application No. WO99/01158). In another embodiment, the therapeutic composition is delivered to the animal by gene gun or gold particle bombardment. In an advantageous embodiment, the animal is a felid. 
     One embodiment of the invention is a method of eliciting an immune response against avian influenza virus in an animal, comprising administering a formulation for delivery and expression of a recombinant poxvirus influenza immunological composition or vaccine or inactivated influenza immunological composition or vaccine in an effective amount for eliciting an immune response. Still another embodiment of the invention is a method of inducing an immunological or a protective immune response against avian influenza virus in an animal, comprising administering to the animal an effective amount of a formulation for delivery and expression of an influenza antigen, epitope or immunogen wherein the formulation comprises recombinant poxvirus influenza immunological composition or vaccine or inactivated influenza immunological composition or vaccine and a pharmaceutically or veterinarily acceptable carrier, vehicle or excipient. 
     The invention relates to a method to elicit, induce or stimulate the immune response of an animal, advantageously a felid. 
     In one embodiment of the invention, the immune response elicited to induced is a protective immune response. As is accepted by those of skill in the art, a protective immune response is one that successfully protects a subject from challenge. 
     Another embodiment of the invention is a kit for performing a method of inducing an immunological or protective response against influenza in an animal comprising a recombinant influenza poxvirus immunological composition or vaccine or an inactivated influenza immunological composition or vaccine and instructions for performing the method of delivery in an effective amount for eliciting an immune response in the animal. 
     The invention will now be further described by way of the following non-limiting examples which further illustrate the invention, and are not intended, nor should they be interpreted to, limit the scope of the invention. 
     EXAMPLES 
     Example 1 
     Construction and Development of TROVAC AIV H5 (vFP89) 
     The construction of vFP89 disclosed herein Example 1 can be found in the following U.S. Pat. Nos. 5,494,807, 5,529,780, 5,688,920, 5,756,102, 5,756,103, 5,762,938, 5,766,599, 5,833,975, 5,863,542, 5,942,235, 6,017,542, 6,265,189, 6,309,647, 6,537,594, 6,596,279, and 6,780,407, the disclosures of which are incorporated by reference. 
     Development of Attenuated Fowlpox Virus 
     Plasmids containing cDNA clones of the H5 hemagglutinin gene was obtained from Dr. Robert Webster, St. Jude Children&#39;s Research Hospital, Memphis, Tenn. The strain of FPV designated FP-1 has been described previously (Taylor et al., 1988a, b). It is a vaccine strain useful in vaccination of day old chickens. The parental virus strain Duvette was obtained in France as a fowlpox scab from a chicken. The virus was attenuated by approximately 50 serial passages in chicken embryonated eggs followed by 25 passages on chick embryo fibroblast (CEF) cells. This virus was obtained in September 1980 by Rhone Merieux, Lyon, France, and a master viral seed established. The virus was received by Virogenetics in September 1989, where it was subjected to four successive plaque purifications. One plaque isolate was further amplified in primary CEF cells and a stock virus, designated as TROVAC, was established. The stock virus used in the in vitro recombination test to produce TROVAC-AIV H5 (vFP89) and TROVAC -AIV H4 (vFP92) had been further amplified though 8 passages in primary CEF cells. The stock virus used to produce TROVAC-AIV H7 (vFP100) had been further amplified through 12 passages in primary CEF cells. 
     Construction of TROVAC Insertion Plasmid at F8 Locus. 
     Plasmid pRW731.15 contains a 10 kbp PvuII-PvuII fragment cloned from TROVAC genomic DNA. The nucleotide sequence was determined on both strands for a 3659 bp PvuII -EcoRV fragment. This sequence is shown in SEQ ID NO: 1. The limits of an open reading frame designated in this laboratory as F8 were determined within this sequence. The open reading frame is initiated at position 495 and terminates at position 1887. A deletion was made from position 779 to position 1926, as described below. With respect to plasmid pRW731.15, reference is made to U.S. Pat. Nos. 5,494,807, 5,529,780, 5,756,102, 5,756,103, 5,766,599, 5,833,975, and 6,596,279, the disclosures of which are incorporated by reference. 
     Plasmid pRW761 is a sub-clone of pRW731.15 containing a 2430 bp EcoRV-EcORV fragment. Plasmid pRW761 was completely digested with XbaI and partially digested with SspI. A 3700 bp XbaI-SspI band was isolated and ligated with the annealed double-stranded oligonucleotides JCA017 (SEQ ID NO: 2) and JCA018 (SEQ ID NO: 3). With respect to plasmid pRW761, reference is made to U.S. Pat. Nos. 5,494,807, 5,529,780, 5,756,102, 5,756,103, 5,766,599, 5,833,975, and 6,596,279, the disclosures of which are incorporated by reference. 
     
       
         
           
               
               
            
               
                   
                 JCA017 (SEQ ID NO: 2) 
               
               
                   
                 5′ CTAGACACTTTATGTTTTTTAATATCCGGTCTTAAA- 
               
               
                   
                 AGCTTCCCGGGGATCCTTATACGGGGAATAAT 3′ 
               
               
                   
                   
               
               
                   
                 JCA018 (SEQ ID NO: 3) 
               
               
                   
                 5′ ATTATTCCCCGTATAAGGATCCCCCGGGAAGCTTTT- 
               
               
                   
                 AAGACCGGATATTAAAAAACATAAAGTGT 3′ 
               
            
           
         
       
     
     The plasmid resulting from this ligation was designated pJCA002. Plasmid pJCA004 contains a non-pertinent gene linked to the vaccinia virus H6 promoter in plasmid pJCA002. The sequence of the vaccinia virus H6 promoter has been previously described (Taylor et al., 1988a, b; Guo et al., 1989; Perkus et al., 1989). Plasmid pJCA004 was digested with EcoRV and BamHI which deletes the non-pertinent gene and a portion of the 3′ end of the H6 promoter. Annealed oligonucleotides RW178 (SEQ ID NO: 4) and RW179 (SEQ ID NO: 5) were cut with EcoRV and BamHI and inserted between the EcoRV and BamHI sites of JCA004 to form pRW846. With respect to plasmids pJCA002, pJCA004, and pRW846, references are made to U.S. Pat. Nos. 5,494,807, 5,529,780, 5,756,102, 5,756,103, 5,766,599, 5,833,975, and 6,596,279, the disclosures of which are incorporated by reference. 
     
       
         
           
               
               
            
               
                   
                 RW178 (SEQ ID NO: 4): 
               
               
                   
                 5′ TCATTATCGCGATATCCGTGTTAACTAGCTAGCTAA 
               
               
                   
                 TTTTTATTCCCGGGATCCTTATCA 3′ 
               
               
                   
               
               
                   
                 RW179 (SEQ ID NO: 5): 
               
               
                   
                 5′ GTATAAGGATCCCGGGAATAAAAATTAGCTAGCTA 
               
               
                   
                 GTTAACACGGATATCGCGATAATGA 3′ 
               
            
           
         
       
     
     Plasmid pRW846 therefore contains the H6 promoter 5′ of EcoRV in the de-ORFed F8 locus. The HincII site 3′ of the H6 promoter in pRW846 is followed by translation stop codons, a transcriptional stop sequence recognized by vaccinia virus early promoters (Yuen et al., 1987) and a SmaI site. 
     Construction of Insertion Plasmid for H5 Hemagglutinin at the F8 Locus in TROVAC 
     A cDNA clone of avian influenza H5 derived from A/Turkey/Ireland/1378/83 was received in plasmid pTH29 from Dr. R. Webster. Synthetic oligonucleotides RW10 (SEQ ID NO: 6) through RW13 (SEQ ID NO: 9) were designed to overlap the translation initiation codon of the previously described vaccinia virus H6 promoter with the ATG of the H5 gene. The sequence continues through the 5′ SalI site of the H5 gene and begins again at the 3′ H5 DraI site containing the H5 stop codon. 
     
       
         
           
               
            
               
                 RW10 (SEQ ID NO. 6) 
               
               
                 5′ GAAAAATTTAAAGTCGACCTGTTTTGTTGAGTTGTTT 
               
               
                 GCGTGGTAACCAATGCAAATCTGGTCACT 3′ 
               
               
                   
               
               
                 RW11 (SEQ ID NO. 7) 
               
               
                 5′ TCTAGCAAGACTGACTATTGCAAAAAGAAGCACTAT 
               
               
                 TTCCTCCATTACGATACAAACTTAACGGAT 3′ 
               
               
                   
               
               
                 RW12 (SEQ ID NO. 8) 
               
               
                 5′ ATCCGTTAAGTTTGTATCGTAATGGAGGAAATAGTGC 
               
               
                 TTCTTTTTGCAATAGTCAGTCTTGCTAGAAGTGACCAGATTTGCATTGGT 
               
               
                 3′ 
               
               
                   
               
               
                 RW13 (SEQ ID NO. 9) 
               
               
                 5′TACCACGCAAACAACTCAACAAAACAGGTCGACTTTA 
               
               
                 AATTTTTCTGCA 3′ 
               
            
           
         
       
     
     The oligonucleotides were annealed at 95° C. for three minutes followed by slow cooling at room temperature. This results in the following double strand structure. 
     Cloning of oligonucleotides between the EcoRV and PstI sites of pRW742B resulted in pRW744. Plasmid pRW742B contains the vaccinia virus H6 promoter linked to a non-pertinent gene inserted at the HincI site of pRW731.15 described previously. Digestion with PstI and EcoRV eliminates the non-pertinent gene and the 3′-end of the H6 promoter. Plasmid pRW744 now contains the 3′ portion of the H6 promoter overlapping the ATG of avian influenza H5. The plasmid also contains the H5 sequence through the 5′ SalI site and the 3′ sequence from the H5 stop codon (containing a DraI site). Use of the DraI site removes the H5 3′ non-coding end. The oligonucleotides add a transcription termination signal recognized by early vaccinia virus RNA polymerase (Yuen et al., 1987). To complete the H6 promoted H5 construct, the H5 coding region was isolated as a 1.6 kpb SalI-DraI fragment from pTH29. Plasmid pRW744 was partially digested with DraI, the linear fragment isolated, recut with SalI and the plasmid now with eight bases deleted between SalI and DraI was used as a vector for the 1.6 kpb pTH29 SalI and DraI fragment. The resulting plasmid pRW759 was cut with EcoRV and DraI. The 1.7 kbp PRW759 EcoRV-DraI fragment containing the 3′ H6 promoter and the H5 gene was inserted between the EcoRV and HincII sites of pRW846 (previously described). The resulting plasmid pRW849 contains the H6 promoted avian influenza virus H5 gene in the de-ORFed F8 locus. With respect to plasmids pRW742B, pRW744, pRW759, and pRW849, references are made to U.S. Pat. Nos. 5,494,807, 5,529,780, 5,756,102, 5,756,103, 5,766,599, 5,833,975, and 6,596,279, the disclosures of which are incorporated by reference. 
     Development of TROVAC-Avian Influenza Virus Recombinants. 
     Insertion plasmids containing the avian influenza virus HA coding sequences were individually transfected into TROVAC infected primary CEF cells by using the calcium phosphate precipitation method previously described (Panicali et al., 1982; Piccini et al., 1987). Positive plaques were selected on the basis of hybridization to HA specific radiolabelled probes and subjected to sequential rounds of plaque purification until a pure population was achieved. One representative plaque was then amplified to produce a stock virus. Plasmid pRW849 was used in an in vitro recombination test to produce recombinant TROVAC-AIV H5 (vFP89) expressing the H5 hemagglutinin. 
     Determination of H5 Expression Using Immunofluorescence. 
     In influenza virus infected cells, the HA molecule is synthesized and glycosylated as a precursor molecule at the rough endoplasmic reticulum. During passage to the plasma membrane it undergoes extensive post-translational modification culminating in proteolytic cleavage into the disulphide linked HA.sub.1 and HA.sub.2 subunits and insertion into the host cell membrane where it is subsequently incorporated into mature viral envelopes. To determine whether the HA molecules produced in cells infected with the TROVAC-AIV recombinant viruses were expressed on the cell surface, immunofluorescence studies were performed. Indirect immunofluorescence was performed as described (Taylor et al., 1990). Surface expression of the H5 hemagglutinin in TROVAC-AIV H5 was confirmed by indirect immunofluorescence. Expression of the H5 hemagglutinin was detected using a pool of monoclonal antibodies specific for the H5 HA. 
     Characterization of H5 Using Immunoprecipitation. 
     It has been determined that the sequence at and around the cleavage site of the hemagglutinin molecule plays an important role in determining viral virulence since cleavage of the hemagglutinin polypeptide is necessary for virus particles to be infectious. The hemagglutinin protein of the virulent H5 possesses more than one basic amino acid at the carboxy terminus of HA1. It is thought that this allows cellular proteases which recognize a series of basic amino acids to cleave the hemagglutinin and allow the infectious virus to spread both in vitro and in vivo. 
     In order to determine that the hemagglutinin molecules expressed by the TROVAC recombinant was authentically processed, immunoprecipitation experiments were performed as described (Taylor et al., 1990) using the specific reagents described above. 
     Immunoprecipitation analysis of the H5 hemagglutinin showed that the glycoprotein is evident as the two cleavage products HA.sub.1 and HA.sub.2 with approximate molecular weights of 44 and 23 kDa, respectively. No such proteins were precipitated from uninfected cells or cells infected with parental TROVAC. Generation of recombinant virus by recombination, in situ hybridization of nitrocellulose filters and screening for B-galactosidase activity are as previously described (Panicali et al., 1982; Perkus et al., 1989). 
     Example 2 
     Construction and Development of TROVAC AIV H5 (vFP2211) 
     AIV H5 HA Gene 
     The nucleotide sequence used in the construction of vFP2211 was derived from AIV A/Chicken/Indonesia/03H5 HA gene supplied by GeneArt GmbH (Regensburg, Germany). The sequence is synthetic with codon optimization for expression in avian cells and with a modification of HA cleavage site (SEQ ID NO: 15, SEQ ID NO: 16). 
     Plasmid Construction of pJY1394.1 
     To construct the donor plasmid pF8 AIV synthetic H5 HA without cleavage site (pJY1394.1), plasmid pRW744 (see Example 1) was partially digested with DraI. The linear fragment was isolated, recut with SalI, and the plasmid now with 8 bases deleted between SalI and DraI was used as a vector for the insertion of the double stranded SalI and DraI fragment comprising the synthetic AIV H5 HA without cleavage site. The resulting plasmid was cut with EcoRV and DraI, providing a 1.8 kbp EcoRV-DraI fragment containing the 3′ H6 promoter and the H5 HA gene. This fragment was inserted between the EcoRV and HincII sites of a donor plasmid based on pRW846 (see Example 1). The resulting plasmid pJY1394.1 contains the vaccinia H6 promoter followed by the synthetic codon-optimized/cleavage site deleted avian influenza virus A/chicken/Indonesia/03H5 HA gene in the de-ORFed F8 locus. With respect to plasmids pRW744 and pRW846, references are made to U.S. Pat. Nos. 5,494,807, 5,529,780, 5,756,102, 5,756,103, 5,766,599, 5,833,975, and 6,596,279, the disclosures of which are incorporated by reference. 
     Generation of TROVAC AIV H5 Recombinants vFP2211 
     To generate vFP2211, plasmid pJY1394.1, which contained the synthetic A/chicken/Indonesia/03H5 HA gene, was linearized with NotI restriction enzyme. The linearized fragments were individually transfected into TROVAC-infected primary CEF cells by using the calcium phosphate precipitation method described previously (Panicali et al. 1982; Piccini et al. 1987). After 42 h, the transfected-infected cells were harvested, sonicated and used for recombinant virus screening. 
     Recombinant plaques were screened based on the plaque lift hybridization method using an AIV-specific probe which was labeled with horse radish peroxidase according to the manufacturer&#39;s protocol (Amersham Cat# RPN-3000). After four sequential rounds of plaque purification, the recombinants, designated as vFP2211 and vFP2211, were generated. vFP2211 was confirmed by hybridization as 100% positive for the AIV insert and 100% negative for the F8 ORF. 
     The vFP2211 recombinants were expanded and concentrated to produce virus stock 
     Analysis of Recombinant vFP2211 
     To re-determine genetic purity, the stock of vFP2211 was re-confirmed by hybridization as 100% positive for the AIV probe and 100% negative for the F8 ORF. The stock of vFP2211 was re-confirmed by hybridization but found to be contaminated with the parental virus. 
     A theoretical restriction enzyme gel for the genomic DNA was created in Vector NTI and is shown in  FIG. 3 . 
     Genomic DNA was extracted and digested with BamHI, HindIII and PstI. The genomic DNA was transferred to nylon membrane and analyses were performed by probing with an AIV probe (see  FIG. 4 ). Bands were observed at the expected sizes, indicating the correct insertion of AIV into the F8 locus (see table 1) 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Size of the bands generated by digestion of genomic DNA with 
               
               
                 restriction enzymes. 
               
            
           
           
               
               
               
            
               
                   
                 Restriction Enzymes 
                 # of bp 
               
               
                   
                   
               
               
                   
                 BamHI 
                 23567 
               
               
                   
                 HindIII 
                 17141 
               
               
                   
                 PstI 
                 24376 
               
               
                   
                   
               
            
           
         
       
     
     Expression of the recombinant was examined through Western Blot Analysis. Primary CEF cells were infected with vFP2211 at MOI of 10 and incubated for 24 hours. The supernatant was harvested and clarified, and the cells were harvested and suspended in water to lyse. Lysate and supernatant were separated by 10% SDS-PAGE. The protein was transferred to nylon membrane and then incubated with HA-specific chicken polyserum TK/W1/68. The AIV -specific proteins were visualized using the Amersham ECL chemiluminescence detection system. The results indicated that the vFP2211 recombinants express the AIV HA genes in the cell lysates (see  FIG. 5 ). There is no HA protein secretion into the supernatant. 
     In addition, an immunoplaque assay of stock using HA-specific chicken polyclonal antiserum TK/W1/68 reveled that the homogeneity of the vFP2211 population was 100% (see  FIG. 6 ) 
     Furthermore, a more detailed analysis of the P2 stock genomic DNA was performed by PCR amplification and sequence analysis of the flanking arms of the F8 locus and the H6p AIV synthetic H5 HA cleavage mutant insert. Primers 11339 (SEQ ID NO: 12) and 11340(SEQ ID NO: 13), located beyond the arms of the F8 locus in the donor plasmid, were used to amplify the entire F8L-insert-F8R fragment (SEQ ID NO: 14; see  FIG. 2 ). The results showed that the sequences of the H6p AIV synthetic H5 HA cleavage mutant insert and the F8 left and right arms in vFP2211 were correct. 
     Example 3 
     Construction and Development of ALVAC AIV H5 (vCP2241) 
     AIV H5 HA Gene 
     The nucleotide sequence used in the construction of vFP2241 was derived from AIV A/Chicken/Indonesia/03H5 HA gene supplied by GeneArt GmbH (Regensburg, Germany). The sequence is contained in plasmid pCR-Script/HA-CK/Indonesia/03-(modified)-avipox and is synthetic with codon optimization for expression in avian cells and with a modification of HA cleavage site (SEQ ID NO: 15, SEQ ID NO: 16). Comparison between the synthetic H5 HA without cleavage site (from plasmid pCR-Script/HA-CK/Indonesia/03-(modified)-avipox) and wild type H5 HA without cleavage site (Ck/Indonesia/2003) indicate that the nucleotide and amino acid sequences were very similar (see  FIGS. 7 ,  8 ) 
     Plasmid Construction of pLH1852.5 
     To construct the ALVAC plasmid pALVAC C 5 H 6 p-AIV synthetic H5 HA without cleavage site (pLH1852.5), the plasmid pJY1394.1 (see Example 2) was digested using EcoRV/SpeI digestion in order to isolate the expression cassette comprised of the H6 promoter and synthetic AIV H5 HA gene. This fragment was then ligated to Eco DNA digested pALVAC C 5 H 6 p donor (pCXL148.2) (see  FIG. 9 ). The resulting plasmid pLH1852.5 ( FIG. 10 ) was confirmed to contain the correct nucleotide (SEQ ID NO: 18) and amino amino acid (SEQ ID NO: 17) sequences. 
     Generation of ALVAC AIV H5 Recombinants vCP2241 
     To generate vCP2241, plasmid pLH1852.5, which contained the synthetic A/Chicken/Indonesia/03H5 HA gene, was linearized with NotI restriction enzyme. The linearized fragments were individually transfected into ALVAC-infected primary CEF cells by using the calcium phosphate precipitation method described previously (Panicali et al. 1982; Piccini et al. 1987) ( FIG. 11 ). After 24 h, the transfected-infected cells were harvested, sonicated and used for recombinant virus screening. 
     Recombinant plaques were screened based on the plaque lift hybridization method using an AIV synthetic H5-specific probe which was labeled with horse radish peroxidase according to the manufacturer&#39;s protocol (Amersham Cat# RPN-3001). After four sequential rounds of plaque purification, the recombinants, designated as vCP2241 was generated and confirmed by hybridization as 100% positive for the AIV synthetic H5 insert and 100% negative for the C5 ORF. 
     A single plaque was selected from the final round of plaque purification and expanded to obtain stocks to amplify vCP2241. The recombinant was re-confirmed by hybridization and was again found to be 100% positive for the AIV synthetic H5 insert and 100% negative for the C5 ORF. The infected cell culture fluid from the roller bottles was harvested and concentrated to produce virus stock. 
     Analysis of Recombinant vCP2241 
     To re-determine genetic purity, vCP2241 was re-confirmed by hybridization as 100% positive for the AIV probe and 100% negative for the C5 ORF. 
     A theoretical restriction enzyme gel for the genomic DNA was created in Vector NTI and is shown in  FIG. 12 . Genomic DNA was extracted from vCP2241 and digested with BamHI, HindIII and PstI, and separated by 0.8% agarose gel electrophoresis. The results revealed the correct insertion of the foreign gene sequence (see  FIG. 13 ). 
     The genomic DNA digested with BamHI, HindIII and PstI was transferred to nylon membrane and Southern blot analysis was performed by probing with AIV synthetic H5 probe. Bands were observed at the expected sizes, indicating the correct insertion of AIV synthetic H5 into the C5 locus ( FIG. 14 ) 
     Expression of the recombinant was examined through Western Blot Analysis. Primary CEF cells were infected vCP2241 at MOI of 10 and incubated at 37° C. for 24 hours. The cells and culture supernatant were then harvested. Sample proteins were separated on a 10% SDS -PAGE gel, transferred to Immobilon nylon membrane, and probed with HA specific chicken polyclonal antiserum (TK/WI/68) at 1:2000. Peroxidase conjugated donkey anti-chicken antiserum was used as a secondary antibody and the bands were visualized using Amersham detection reagents. One protein band was detected in the cell pellets of vCP2241 and vFP2211, indicating the expression of the hemagglutinin protein. The expressed protein did not secrete into the cell culture ( FIG. 15 ) 
     The homogeneity of the vCP2241 population was 100% as evidenced by an immunoplaque assay, using HA-specific chicken polyclonal antiserum TK/WI/68 ( FIG. 16 ). 
     A more detailed analysis of the P3 stock genomic DNA was performed by PCR amplification and sequence analysis of the flanking arms of the C5 locus and the AIV synthetic H5 insert. Primers 7931.DC (SEQ ID NO. 20).and 7932.DC (SEQ ID NO: 21) located beyond the arms of the C5 locus (SEQ ID NO. 22,  FIG. 17 ), were used to amplify the entire C5R— AIV synthetic H5 insert-C5L fragment. The results showed that the sequence of the AIV synthetic H5 insert and the C5 left and right arms around the AIV synthetic H5 insert in vCP2241 was correct. 
     Example 4 
     Vaccination of Cats with vFP89 (TROVAC AIV-H5) 
     A study was conducted in which 20 cats, aged 16-18 weeks-old, were randomly divided into 2 groups. The first group was immunized with an attenuated fowlpox (TROVAC) expressing H5 genes from the avian influenza strain A/Turkey/Ireland/1378/83 (vFP89), while the second groups served as a control. On day 0 and day 29, the vaccinated cats were subcutaneously administered 6.8 log10 of 50% cell culture infective dose, per dose (CCID50/dose). 
     Blood samples were collected from all cats 3 days prior to the first vaccination, and on days 7, 14, 21, 29, 35, and 42 after the first vaccination. A hemagglutination inhibition (HI) test was performed as described previously (Swayne et al., 1997). Briefly, chicken red blood cell-(cRBC)-treated cat serum was serially diluted and then incubated in wells with 4 HA units of homologous (H5N8 AIV A/Turkey/Ireland/1378/83) and heterologous (H5N1 AIV A/Chicken/Indonesia/03) H5 AIV antigens. A 0.5% (vol/vol) suspension of cRBCs were allotted per well. Antibodies titers corresponding to the reciprocal of the highest dilution that inhibited hemagglutination were presented as geometric mean titers (GMTs). 
     Vaccination did not induce systemic or local adverse reactions. Antibodies to homologous AIV antigen were first detected in 8 of 10 cats at 1 week, and all cats thereafter. The GMTs at days 14, 21, and 29 were 91, 97, and 79, respectively. After the second dose on day 29, the GMTs on day 35 and 42 were 446. 
     Antibodies to heterologous AIV antigen were detectable after the second vaccination on days 35 and 42, in which the GMTs were 34 and 39, respectively. 
     Together, these results indicate that the vaccines induced high levels of antibodies to H5 avian influenza virus. 
     Example 5 
     Vaccination of Cats with vFP89 (TROVAC AIV-H5) vFP2211 (TROVAC AIV H5) and vCP2241 (ALVAC AIV H5) 
     A study was conducted in which 24 cats, aged 16-18 weeks old, were randomly divided into 4 groups. Three groups were immunized with either an attenuated fowlpox (TROVAC) expressing H5 gene from the avian influenza strain A/Turkey/Ireland/1378/83 (vFP89), an attenuated fowlpox (TROVAC) expressing H5 gene from the avian influenza strain A/Chicken/Indonesia/03 (vFP2211), or an attenuated canarypox (ALVAC) expressing H5 gene from the avian influenza strain A/Chicken/Indonesia/03 (vCP2241). The fourth group was unvaccinated and served as a control. On day 0 and day 21, the vaccinated cats received subcutaneous injections in the interscapula area of approximately 7.2 log10 of 50% cell culture infective dose, per dose (CCID50/dose). 
     The vaccines were tirated, as shown in Table 2. 
     
       
         
           
               
               
               
               
             
               
                   
               
               
                   
                   
                 Injection 
                 Injection titer 
               
               
                   
                   
                 titer at D0 
                 at D21 
               
               
                   
                   
                 (log10 CCID 50 / 
                 (log10 CCID 50 / 
               
               
                 Group 
                 Vectors 
                 dose) 
                 dose) 
               
               
                   
               
             
            
               
                 vFP89 
                 TROVAC AIV H5 vector 
                 7.0 
                 6.7 
               
               
                   
                 A/Turkey/Ireland/1378/83 
               
               
                 vFP2211 
                 TROVAC AIV H5 vector 
                 7.4 
                 7.1 
               
               
                   
                 A/chicken/Indonesia/03 
               
               
                 vCP2241 
                 ALVAC AIV H5 vector 
                 6.4 
                 6.7 
               
               
                   
                 A/chicken/Indonesia/03 
               
               
                   
               
            
           
         
       
     
     Blood samples were collected on days 0, 15, 21, and 35 for all groups, and additionally on day 84 for the vaccinated groups. A hemagglutination inhibition (HI) test was performed as described in Example 4. For this study, the homologous AIV antigen was H5N8 AIV A/Turkey/Ireland/1378/83, and the heterologous antigen was H5N1 AIV A/Vietnam/1194/04 (NIBRG14 strain). A 0.5% (vol/vol) suspension of cRBCs were allotted per well. The results are presented as log 10 of the highest dilution of cat serum that inhibited hemaggluntination. 
     Injection with the recombinant vaccines induced high levels of antibodies to H5 avian influenza virus. Among the vaccined groups, cats injected with the vFP89 plasmid displayed higher HI responses against both the homologous and heterologus antigens (see  FIGS. 18 and 19 ). In addition, vCP2241 showed greater HI responses than vFP2211 against both types of antigens. Nonetheless, all three vaccines induced an immunogenic response in cats. 
     Example 6 
     Vaccination of Cats with Inactivated AIV 
     A study was conducted in which two groups of 20 week-old cats received vaccines comparising inactivated AIV. The viruses (H5N9 AIV A/Chicken/Italy/22A/98) were inactivated by 0.1% beta-propiolactone at 5° C. for 18 hours, and were then harvested and filtated through a 10 μm cutoff filter. The vaccine was adjuvanted with oil-in-water emulsion. 
     All cats experienced subcutaneous injections in the interscapula area of a 1 ml dose on days 0 and 21. The first group (n=5) received approximately 512 HA units (UHA) of inactivated vaccine, while the second group (n=5) received approximately 1536 UHA of inactivated vaccine. 
     Blood samples were collected on days 0, 14, 21, and 35 for all animals. A hemagglutination inhibition (HI) test was performed as described in Example 4. For this study, the homologous AIV antigen was H5N9 AIV A/Turkey/Wisconsin/68, and the heterologous antigen was H5N1 AIV A/Vietnam/1194/04 (NIBRG14 strain). A 0.5% (vol/vol) suspension of cRBCs were allotted per well. The results are presented as log 10 of the highest dilution of cat serum that inhibited hemaggluntination. 
     The results indicate that cats injected with the inactivated vaccine induced high levels of antibodies to H5 avian influenza. While cats injected with 512 UHA/dose displayed higher HI responses against both homologous (see  FIG. 20 ) and heterologous (see  FIG. 21 ) antigens than cats injected with 1536 UHA/dose, both dosages induced an immunogenic response. 
     The invention is further described by the following numbered paragraphs: 
     1. A method of eliciting an immune response against influenza in a Felidae, comprising administering a formulation, wherein said formulation comprises an avipox expression vector comprising a polynucleotide encoding an influenza antigen, epitope or immunogen in an effective amount for eliciting an immune response. 
     2. A method of inducing a protective immune response against influenza in a Felidae, comprising administering a formulation, wherein said formulation comprises an avipox expression vector, wherein said avipox expression vector comprises a polynucleotide encoding an influenza antigen, epitope or immunogen in an effective amount for inducing a protective immune response. 
     3. The method of paragraph 1 or 2, wherein the formulation further comprises one or more of a pharmaceutically or veterinarily acceptable carrier, excipient, vehicle or adjuvant. 
     4. The method of any one of paragraphs 1 to 3, wherein the influenza antigen, epitope or immunogen is a hemagglutinin, matrix protein, membrane protein, neuraminidase, nonstructural protein, nucleoprotein, polymerase or any fragment thereof. 
     5. The method of any one of paragraphs 1 to 4, wherein the influenza antigen, epitope or immunogen is isolated from a feline infected with influenza. 
     6. The method of paragraph 5 wherein the influenza antigen, epitope or immunogen is isolated from the broncho alveolar lavage and/or lung tissues of the feline. 
     7. The method of any one of paragraphs 1 to 4, wherein the influenza antigen, epitope or immunogen is isolated from an avian influenza. 
     8. The method of any one of paragraphs 1 to 7, wherein the avipox expression vector is an attenuated avipox expression vector. 
     9. The method of paragraph 8, wherein the avipox expression vector is a fowlpox vector. 
     10. The method of paragraph 9, wherein the fowlpox vector is a TROVAC vector. 
     11. The method of paragraph 8, wherein the avipox expression vector is a canarypox vector. 
     12. The method of paragraph 11, wherein the canarypox vector is ALVAC. 
     13. The method of paragraph 9, 10, 11, or 12, wherein the influenza antigen, epitope or immunogen is a hemagglutinin. 
     14. The method of paragraph 13, wherein the hemagglutinin is H5. 
     15. The method of paragraph 13 or 14, wherein the fowlpox vector is FP89 or FP2211. 
     16. The method of paragraph 13 or 14, wherein the canarypox vector is CP2241. 
     17. A method of eliciting an immune response against influenza in a Felidae, comprising administering a formulation, wherein said formulation comprises an inactivated influenza vaccine in an effective amount for eliciting an immune response. 
     18. A method of inducing a protective immune response against influenza in a Felidae, comprising administering a formulation, wherein said formulation comprises an inactivated influenza vaccine in an effective amount for inducing an immune response. 
     19. The method of paragraph 17 or 18, wherein the formulation further comprises one or more of a pharmaceutically or veterinarily acceptable carrier, excipient, vehicle or adjuvant. 
     20. The method of any one of paragraph 17 to 19, wherein the inactivated influenza vaccine is an inactivated feline influenza. 
     21. The method of any one of paragraphs 17 to 19, wherein the inactivated influenza vaccine is an inactivated avian influenza. 
     22. The method of any one of claims  1 - 21 , further comprising additionally administering, either prior or subsequent to said formulation, a second formulation comprising either an avipox expression vector, wherein said vector comprises a polynucleotide encoding an influenza antigen, or an inactivated influenza immunological composition. 
     23. An immunological composition comprising a formulation, wherein said formulation comprises an avipox expression vector comprising a polynucleotide encoding an influenza antigen, epitope or immunogen, and a pharmaceutically or veterinarily acceptable carrier, excipient or vehicle in an effective amount for inducing an immune response. 
     24. The immunological composition of paragraph 23, wherein the formulation further comprises an adjuvant. 
     25. The immunological composition of paragraph 23 or 24, wherein the influenza antigen, epitope or immunogen is a hemagglutinin, matrix protein, membrane protein, neuraminidase, nonstructural protein, nucleoprotein, polymerase or any fragment thereof. 
     26. The immunological composition of any one of paragraphs 23 to 25, wherein the influenza antigen, epitope or immunogen is isolated from a feline infected with influenza. 
     27. The immunological composition of paragraph 26 wherein the influenza antigen, epitope or immunogen is isolated from the broncho alveolar lavage and/or lung tissues of the feline. 
     28. The immunological composition of any one of paragraphs 23 to 25, wherein the influenza antigen, epitope or immunogen is isolated from an avian influenza. 
     29. The immunological composition of any one of paragraphs 23 to 28, wherein the avipox expression vector is an attenuated avipox expression vector. 
     30. The immunological composition of paragraph 29, wherein the avipox expression vector is a fowlpox vector. 
     31. The immunological composition of paragraph 30, wherein the fowlpox vector is a TROVAC vector. 
     32. The immunological composition of paragraph 29, wherein the avipox expression vector is a canarypox vector. 
     33. The immunological composition of paragraph 32, wherein the canarypox vector is ALVAC. 
     34. The immunological composition of any one of paragraphs 30-33, wherein the influenza antigen, epitope or immunogen is a hemagglutinin. 
     35. The immunological composition of paragraph 34, wherein the hemagglutinin is H5. 
     36. The immunological composition of paragraph 34 or 35, wherein the fowlpox vector is vFP89 or vFP2211. 
     37. The immunological composition of paragraph 34 or 35, wherein the canarypox vector is vCP2241. 
     37. A kit for performing any one of the methods of paragraphs 1 to 21 comprising the immunological composition of any one of paragraphs 22 to 36 and instructions for performing the method of any one of paragraphs 1 to 36. 
     Having thus described in detail preferred embodiments of the present invention, it is to be understood that the invention defined by the above paragraphs is not to be limited to particular details set forth in the above description as many apparent variations thereof are possible without departing from the spirit or scope of the present invention. 
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                 SEQ ID NO 1 
                   
               
               
                 LENGTH: 3659 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Fowlpox virus 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 gatatctgtg gtctatatat actacaccct accgatatta accaacgagt ttctcacaag 
                   
               
               
                   
               
               
                 60 
                 aaaacttgtt tagtagatag agattctttg attgtgttta aaagaagtac cagtaaaaag 
               
               
                   
               
               
                 120 
                 tgtggcatat gcatagaaga aataaacaaa aaacatattt ccgaacagta ttttggaatt 
               
               
                   
               
               
                 180 
                 ctcccaagtt gtaaacatat tttttgccta tcatgtataa gacgttgggc agatactacc 
               
               
                   
               
               
                 240 
                 agaaatacag atactgaaaa tacgtgtcct gaatgtagaa tagtttttcc tttcataata 
               
               
                   
               
               
                 300 
                 cccagtaggt attggataga taataaatat gataaaaaaa tattatataa tagatataag 
               
               
                   
               
               
                 360 
                 aaaatgattt ttacaaaaat acctataaga acaataaaaa tataattaca tttacggaaa 
               
               
                   
               
               
                 420 
                 atagctggtt ttagtttacc aacttagagt aattatcata ttgaatctat attgtttttt 
               
               
                   
               
               
                 480 
                 agttatataa aaacatgatt agcccccaat cggatgaaaa tataaaagat gttgagaatt 
               
               
                   
               
               
                 540 
                 tcgaatacaa caaaaagagg aatcgtacgt tgtccatatc caaacatata aataaaaatt 
               
               
                   
               
               
                 600 
                 caaaagtagt attatactgg atgtttagag atcaacgtgt acaagataat tgggctttaa 
               
               
                   
               
               
                 660 
                 tttacgcaca acgattagcg ttaaaactca aaatacctct aagaatatgc ttttgtgtcg 
               
               
                   
               
               
                 720 
                 tgccaaaatt tcacactact acttctagac actttatgtt tttaatatcc ggtcttaaag 
               
               
                   
               
               
                 780 
                 aagtcgcgga agaatgtaaa agactatgta tagggttttc attgatatat ggcgtaccaa 
               
               
                   
               
               
                 840 
                 aagtaataat tccgtgtata gtaaaaaaat acagagtcgg agtaatcata acggatttct 
               
               
                   
               
               
                 900 
                 ttccattacg tgttcccgaa agattaatga aacagactgt aatatctctt ccagataaca 
               
               
                   
               
               
                 960 
                 taccttttat acaagtagac gctcataata tagtaccttg ttgggaagct tctgataaag 
               
               
                   
               
               
                 1020 
                 aagaatacgg tgcacgaact ttaagaaaaa agatatttga taaattatat gaatatatga 
               
               
                   
               
               
                 1080 
                 cagaatttcc tgttgttcgt aaacatccat acggtccatt ttctatatct attgcaaaac 
               
               
                   
               
               
                 1140 
                 ccaaaaatat atcattagac aagacggtat tacccgtaaa atgggcaacg cctggaacaa 
               
               
                   
               
               
                 1200 
                 aagctggaat aattgtttta aaagaattta taaaaaacag attaccgtca tacgacgcgg 
               
               
                   
               
               
                 1260 
                 atcataacaa tcctacgtgt gacgctttga gtaacttatc tccgtggcta cattttggtc 
               
               
                   
               
               
                 1320 
                 atgtatccgc acaacgtgtt gccttagaag tattaaaatg tatacgagaa agcaaaaaaa 
               
               
                   
               
               
                 1380 
                 acgttgaaac gtttatagat gaaataattg taagaagaga actatcggat aatttttgtt 
               
               
                   
               
               
                 1440 
                 actataacaa acattatgat agtatccagt ctactcattc atgggttaga aaaacattag 
               
               
                   
               
               
                 1500 
                 aagatcacat taatgatcct agaaagtata tatattccat taaacaactc gaaaaagcgg 
               
               
                   
               
               
                 1560 
                 aaactcatga tcctctatgg aacgcgtcac aaatgcagat ggtgagagaa ggaaaaatgc 
               
               
                   
               
               
                 1620 
                 atagtttttt acgaatgtat tgggctaaga agatacttga atggactaga acacctgaag 
               
               
                   
               
               
                 1680 
                 acgctttgag ttatagtatc tatttgaaca acaagtacga actagacggc acggatccta 
               
               
                   
               
               
                 1740 
                 acggatacgt aggttgtatg tggtctattt gcggattaca cgatagagcg tggaaagcaa 
               
               
                   
               
               
                 1800 
                 gaccgatatt tggaaagata agatatatga attatgagag ttctaagaag aaatttgatg 
               
               
                   
               
               
                 1860 
                 ttgctgtatt tatacagaaa tacaattaag ataaataata tacagcattg taaccatcgt 
               
               
                   
               
               
                 1920 
                 catccgttat acggggaata atattaccat acagtattat taaattttct tacgaagaat 
               
               
                   
               
               
                 1980 
                 atagatcggt atttatcgtt agtttatttt acatttatta attaaacatg tctactatta 
               
               
                   
               
               
                 2040 
                 cctgttatgg aaatgacaaa tttagttata taatttatga taaaattaag ataataataa 
               
               
                   
               
               
                 2100 
                 tgaaatcaaa taattatgta aatgctacta gattatgtga attacgagga agaaagttta 
               
               
                   
               
               
                 2160 
                 cgaactggaa aaaattaagt gaatctaaaa tattagtcga taatgtaaaa aaaataaatg 
               
               
                   
               
               
                 2220 
                 ataaaactaa ccagttaaaa acggatatga ttatatacgt taaggatatt gatcataaag 
               
               
                   
               
               
                 2280 
                 gaagagatac ttgcggttac tatgtacacc aagatctggt atcttctata tcaaattgga 
               
               
                   
               
               
                 2340 
                 tatctccgtt attcgccgtt aaggtaaata aaattattaa ctattatata tgtaatgaat 
               
               
                   
               
               
                 2400 
                 atgatatacg acttagcgaa atggaatctg atatgacaga agtaatagat gtagttgata 
               
               
                   
               
               
                 2460 
                 aattagtagg aggatacaat gatgaaatag cagaaataat atatttgttt aataaattta 
               
               
                   
               
               
                 2520 
                 tagaaaaata tattgctaac atatcgttat caactgaatt atctagtata ttaaataatt 
               
               
                   
               
               
                 2580 
                 ttataaattt tataaatttt aataaaaaat acaataacga cataaagata tttaatcttt 
               
               
                   
               
               
                 2640 
                 aattcttgat ctgaaaaaca catctataaa actagataaa aagttattcg ataaagataa 
               
               
                   
               
               
                 2700 
                 taatgaatcg aacgatgaaa aattggaaac agaagttgat aagctaattt ttttcatcta 
               
               
                   
               
               
                 2760 
                 aatagtatta ttttattgaa gtacgaagtt ttacgttaga taaataataa aggtcgattt 
               
               
                   
               
               
                 2820 
                 ttactttgtt aaatatcaaa tatgtcatta tctgataaag atacaaaaac acacggtgat 
               
               
                   
               
               
                 2880 
                 tatcaaccat ctaacgaaca gatattacaa aaaatacgtc ggactatgga aaacgaagct 
               
               
                   
               
               
                 2940 
                 gatagcctca atagaagaag cattaaagaa attgttgtag atgttatgaa gaattgggat 
               
               
                   
               
               
                 3000 
                 catcctcaac gaagaaatag ataaagttct aaactggaaa aatgatacat taaacgattt 
               
               
                   
               
               
                 3060 
                 agatcatcta aatacagatg ataatattaa ggaaatcata caatgtctga ttagagaatt 
               
               
                   
               
               
                 3120 
                 tgcgtttaaa aagatcaatt ctattatgta tagttatgct atggtaaaac tcaattcaga 
               
               
                   
               
               
                 3180 
                 taacgaacat tgaaagataa aattaaggat tattttatag aaactattct taaagacaaa 
               
               
                   
               
               
                 3240 
                 cgtggttata aacaaaagcc attacccgga ttggaaacta aaatactaga tagtattata 
               
               
                   
               
               
                 3300 
                 agattttaaa aacataaaat taataggttt ttatagattg acttattata tacaatatgg 
               
               
                   
               
               
                 3360 
                 ataaaagata tatatcaact agaaagttga atgacggatt cttaatttta tattatgatt 
               
               
                   
               
               
                 3420 
                 caatagaaat tattgtcatg tcgtgtaatc attttataaa tatatcagcg ttactagcta 
               
               
                   
               
               
                 3480 
                 agaaaaacaa ggactttaat gaatggctaa agatagaatc atttagagaa ataatagata 
               
               
                   
               
               
                 3540 
                 ctttagataa aattaattac gatctaggac aacgatattg tgaagaactt acggcgcatc 
               
               
                   
               
               
                 3600 
                 acattccagt gtaattattg aggtcaaagc tagtaactta atagatgaca ggacagctg 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 2 
                   
               
               
                 LENGTH: 68 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Doublestranded oligonucleotide referred to as JCA017 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 ctagacactt tatgtttttt aatatccggt cttaaaagct tcccggggat ccttatacgg 
                   
               
               
                   
               
               
                 60 
                 ggaataat 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 3 
                   
               
               
                 LENGTH: 65 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Doublestranded oligonucleotide referred to as JCA018 
               
               
                   
               
               
                 SEQUENCE: 38 
               
            
           
           
               
               
               
            
               
                 1 
                 attattcccc gtataaggat cccccgggaa gcttttaaga ccggatatta aaaaacataa 
                   
               
               
                   
               
               
                 60 
                 agtgt 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 4 
                   
               
               
                 LENGTH: 60 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Oligonucleotide referred to as RW178 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 tcattatcgc gatatccgtg ttaactagct agctaatttt tattcccggg atccttatca 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 5 
                   
               
               
                 LENGTH: 60 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Oligonucleotide referred to as RW179 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 gtataaggat cccgggaata aaaattagct agctagttaa cacggatatc gcgataatga 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 6 
                   
               
               
                 LENGTH: 66 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Synthetic oligonucleotide referred to as RW10 
               
               
                   
               
               
                 SEQUENCE: 58 
               
            
           
           
               
               
               
            
               
                 1 
                 gaaaaattta aagtcgacct gttttgttga gttgtttgcg tggtaaccaa tgcaaatctg 
                   
               
               
                   
               
               
                 60 
                 gtcact 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 7 
                   
               
               
                 LENGTH: 66 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Synthetic oligonucleotide referred to as RW11 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 tctagcaaga ctgactattg caaaaagaag cactatttcc tccattacga tacaaactta 
                   
               
               
                   
               
               
                 60 
                 acggat 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 8 
                   
               
               
                 LENGTH: 87 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Synthetic oligonucleotide referred to as RW12 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 atccgttaag tttgtatcgt aatggaggaa atagtgcttc tttttgcaat agtcagtctt 
                   
               
               
                   
               
               
                 60 
                 gctagaagtg accagatttg cattggt 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 9 
                   
               
               
                 LENGTH: 49 
                   
               
               
                   
               
               
                 TYPE: DNA 
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Synthetic oligonucleotide referred to as RW13 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 taccacgcaa acaactcaac aaaacaggtc gactttaaat ttttctgca 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 10 
                   
               
               
                 LENGTH: 21 
                   
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Primer for amplifying AIV probe referred to as 11526JY 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 ACGAAGCCAGCAGCGGAGTGA 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 11 
                   
               
               
                 LENGTH: 21 
                   
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Primer for amplifying AIV probe referred to as 11531JY 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 TCAGCACCAGCAGTTCGGCGT 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 12 
                   
               
               
                 LENGTH: 24 
                   
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Primer for PCR amplification of the F8 arms plus insert, 
               
               
                 referred to as 11339CXL 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 GTAGTGATCAAAATACAGAACCAT 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 13 
                   
               
               
                 LENGTH: 24 
                   
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Primer for PCR amplification of the F8 arms plus insert, 
               
               
                 referred to as 11340CXL 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 GAATCCGTCATTCAACTTTCTAGT 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 14 
                   
               
               
                 LENGTH: 4737 
                   
               
               
                   
               
               
                 OTHER INFORMATION: F8 right arm (1-1429), H6 promoter (1516-1639), AIV 
               
               
                 synethetic H5 HA (1640-3334), and F8 left arm (3362-4737) determined through 
               
               
                 sequence analysis 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 GACCCTTTAC AAGAATAAAA GAAGAAACAA CTGTGAAATA GTTTATAAAT 
                   
               
               
                   
                 CTGGGAAATG TTCTTATTTT CTTCTTTGTT GACACTTTAT CAAATATTTA 
               
               
                   
               
               
                 51 
                 GTAATTCGTA TGCAGAAAAC GATAATATAT TTTGGTATGA GAAATCTAAA 
               
               
                   
                 CATTAAGCAT ACGTCTTTTG CTATTATATA AAACCATACT CTTTAGATTT 
               
               
                   
               
               
                 101 
                 GGAGACATAG TTTGTATAGA CATGCGCTCT TCCGATGAGA TATTCGATGC 
               
               
                   
                 CCTCTGTATC AAACATATCT GTACGCGAGA AGGCTACTCT ATAAGCTACG 
               
               
                   
               
               
                 151 
                 TTTTCTAATG TATCATATAG CTACAAGATA TGCCTATCAT GATGATGATA 
               
               
                   
                 AAAAGATTAC ATAGTATATC GATGTTCTAT ACGGATAGTA CTACTACTAT 
               
               
                   
               
               
                 201 
                 TATATCTACA AATAGTGTTA TATTATTCTA ATAATCAAAA TGTTATATCT 
               
               
                   
                 ATATAGATGT TTATCACAAT ATAATAAGAT TATTAGTTTT ACAATATAGA 
               
               
                   
               
               
                 251 
                 TATATTACGA AAAATAAATA CGTTAAGTAT ATAAGAAATA AAACTAGAGA 
               
               
                   
                 ATATAATGCT TTTTATTTAT GCAATTCATA TATTCTTTAT TTTGATCTCT 
               
               
                   
               
               
                 301 
                 CGATATTCAT AAAGTAAAAA TATTAGCTCT AGAAGACTTT ACAACGGAAG 
               
               
                   
                 GCTATAAGTA TTTCATTTTT ATAATCGAGA TCTTCTGAAA TGTTGCCTTC 
               
               
                   
               
               
                 351 
                 AAATATATTG TTGGATTAGT AATATATAAC AGCGTAGCTG CACGGTTTTG 
               
               
                   
                 TTTATATAAC AACCTAATCA TTATATATTG TCGCATCGAC GTGCCAAAAC 
               
               
                   
               
               
                 401 
                 ATCATTTTCC AACAATATAA ACCAATGAAG GAGGACGACT CATCAAACAT 
               
               
                   
                 TAGTAAAAGG TTGTTATATT TGGTTACTTC CTCCTGCTGA GTAGTTTGTA 
               
               
                   
               
               
                 451 
                 AAATAACATT CACGGAAAAT ATTCAGTATC AGATTTATCA CAAGATGATT 
               
               
                   
                 TTTATTGTAA GTGCCTTTTA TAAGTCATAG TCTAAATAGT GTTCTACTAA 
               
               
                   
               
               
                 501 
                 ATGTTATTGA ATGTATAGAC GGATCTTTTG ATTCGATCAA GTATAGAGAT 
               
               
                   
                 TACAATAACT TACATATCTG CCTAGAAAAC TAAGCTAGTT CATATCTCTA 
               
               
                   
               
               
                 551 
                 ATAAAGGTTA TAATAATGAA GAATAACGGT TACGTTAATT GTAGTAAATT 
               
               
                   
                 TATTTCCAAT ATTATTACTT CTTATTGCCA ATGCAATTAA CATCATTTAA 
               
               
                   
               
               
                 601 
                 ATGTAAAATG CGGAATAAAT ACTTTTCTAG ATGGTTGCGT CTTTCTACTT 
               
               
                   
                 TACATTTTAC GCCTTATTTA TGAAAAGATC TACCAACGCA GAAAGATGAA 
               
               
                   
               
               
                 651 
                 CTAAAGCATT ATTAGACATT TACAATAATA AGTCAGTAGA TAATGCTATT 
               
               
                   
                 GATTTCGTAA TAATCTGTAA ATGTTATTAT TCAGTCATCT ATTACGATAA 
               
               
                   
               
               
                 701 
                 GTTAAAGTCT ATGGTAAAGG TAAGAAACTT ATTATAACAG GATTTTATCT 
               
               
                   
                 CAATTTCAGA TACCATTTCC ATTCTTTGAA TAATATTGTC CTAAAATAGA 
               
               
                   
               
               
                 751 
                 CAAACAAAAT ATGATACGTT ATGTTATTGA GTGGATAGGG GATGATTTTA 
               
               
                   
                 GTTTGTTTTA TACTATGCAA TACAATAACT CACCTATCCC CTACTAAAAT 
               
               
                   
               
               
                 801 
                 CAAACGATAT ATACAAAATG ATTAATTTCT ATAATGCGTT ATTCGGTAAC 
               
               
                   
                 GTTTGCTATA TATGTTTTAC TAATTAAAGA TATTACGCAA TAAGCCATTG 
               
               
                   
               
               
                 851 
                 GATGAATTAA AAATAGTATC CTGTGAAAAC ACTCTATGCC CGTTTATAGA 
               
               
                   
                 CTACTTAATT TTTATCATAG GACACTTTTG TGAGATACGG GCAAATATCT 
               
               
                   
               
               
                 901 
                 ACTTGGTAGA TGCTATTATG GTAAAAAATG TAAGTATATA CACGGAGATC 
               
               
                   
                 TGAACCATCT ACGATAATAC CATTTTTTAC ATTCATATAT GTGCCTCTAG 
               
               
                   
               
               
                 951 
                 AATGTGATAT CTGTGGTCTA TATATACTAC ACCCTACCGA TATTAACCAA 
               
               
                   
                 TTACACTATA GACACCAGAT ATATATGATG TGGGATGGCT ATAATTGGTT 
               
               
                   
               
               
                 1001 
                 CGAGTTTCTC ACAAGAAAAC TTGTTTAGTA GATAGAGATT CTTTGATTGT 
               
               
                   
                 GCTCAAAGAG TGTTCTTTTG AACAAATCAT CTATCTCTAA GAAACTAACA 
               
               
                   
               
               
                 1051 
                 GTTTAAAAGA AGTACCAGTA AAAAGTGTGG CATATGCATA GAAGAAATAA 
               
               
                   
                 CAAATTTTCT TCATGGTCAT TTTTCACACC GTATACGTAT CTTCTTTATT 
               
               
                   
               
               
                 1101 
                 ACAAAAAACA TATTTCCGAA CAGTATTTTG GAATTCTCCC AAGTTGTAAA 
               
               
                   
                 TGTTTTTTGT ATAAAGGCTT GTCATAAAAC CTTAAGAGGG TTCAACATTT 
               
               
                   
               
               
                 1151 
                 CATATTTTTT GCCTATCATG TATAAGACGT TGGGCAGATA CTACCAGAAA 
               
               
                   
                 GTATAAAAAA CGGATAGTAC ATATTCTGCA ACCCGTCTAT GATGGTCTTT 
               
               
                   
               
               
                 1201 
                 TACAGATACT GAAAATACGT GTCCTGAATG TAGAATAGTT TTTCCTTTCA 
               
               
                   
                 ATGTCTATGA CTTTTATGCA CAGGACTTAC ATCTTATCAA AAAGGAAAGT 
               
               
                   
               
               
                 1251 
                 TAATACCCAG TAGGTATTGG ATAGATAATA AATATGATAA AAAAATATTA 
               
               
                   
                 ATTATGGGTC ATCCATAACC TATCTATTAT TTATACTATT TTTTTATAAT 
               
               
                   
               
               
                 1301 
                 TATAATAGAT ATAAGAAAAT GATTTTTACA AAAATACCTA TAAGAACAAT 
               
               
                   
                 ATATTATCTA TATTCTTTTA CTAAAAATGT TTTTATGGAT ATTCTTGTTA 
               
               
                   
               
               
                 1351 
                 AAAAATATAA TTACATTTAC GGAAAATAGC TGGTTTTAGT TTACCAACTT 
               
               
                   
                 TTTTTATATT AATGTAAATG CCTTTTATCG ACCAAAATCA AATGGTTGAA 
               
               
                   
               
               
                 1401 
                 AGAGTAATTA TCATATTGAA TCTATATTGC TAATTAGCTA ATAAAAACCC 
               
               
                   
                 TCTCATTAAT AGTATAACTT AGATATAACG ATTAATCGAT TATTTTTGGG 
               
               
                   
               
               
                 1451 
                 GGGTTAATTA ATTAGTCATC AGGCAGGGCG AGAACGAGAC TATCTGCTCG 
               
               
                   
                 CCCAATTAAT TAATCAGTAG TCCGTCCCGC TCTTGCTCTG ATAGACGAGC 
               
               
                   
               
               
                 1501 
                 TTAATTAATT AGAGCTTCTT TATTCTATAC TTAAAAAGTG AAAATAAATA 
               
               
                   
                 AATTAATTAA TCTCGAAGAA ATAAGATATG AATTTTTCAC TTTTATTTAT 
               
               
                   
               
               
                 1551 
                 CAAAGGTTCT TGAGGGTTGT GTTAAATTGA AAGCGAGAAA TAATCATAAA 
               
               
                   
                 GTTTCCAAGA ACTCCCAACA CAATTTAACT TTCGCTCTTT ATTAGTATTT 
               
               
                   
               
               
                 1601 
                 TTATTTCATT ATCGCGATAT CCGTTAAGTT TGTATCGTAA TGGAGAAAAT 
               
               
                   
                 AATAAAGTAA TAGCGCTATA GGCAATTCAA ACATAGCATT ACCTCTTTTA 
               
               
                   
               
               
                 1651 
                 CGTGCTGCTG CTGGCCATCG TGAGCCTGGT GAAAAGCGAT CAGATCTGCA 
               
               
                   
                 GCACGACGAC GACCGGTAGC ACTCGGACCA CTTTTCGCTA GTCTAGACGT 
               
               
                   
               
               
                 1701 
                 TCGGCTACCA CGCCAACAAC AGCACAGAGC AAGTGGACAC AATCATGGAA 
               
               
                   
                 AGCCGATGGT GCGGTTGTTG TCGTGTCTCG TTCACCTGTG TTAGTACCTT 
               
               
                   
               
               
                 1751 
                 AAGAACGTGA CCGTGACACA CGCCCAGGAC ATCCTGGAAA AGACACACAA 
               
               
                   
                 TTCTTGCACT GGCACTGTGT GCGGGTCCTG TAGGACCTTT TCTGTGTGTT 
               
               
                   
               
               
                 1801 
                 CGGGAAGCTG TGCGATCTGG ATGGAGTGAA GCCTCTGATC CTGAGAGATT 
               
               
                   
                 GCCCTTCGAC ACGCTAGACC TACCTCACTT CGGAGACTAG GACTCTCTAA 
               
               
                   
               
               
                 1851 
                 GCAGCGTGGC CGGATGGCTG CTGGGGAACC CAATGTGCGA CGAATTCATC 
               
               
                   
                 CGTCGCACCG GCCTACCGAC GACCCCTTGG GTTACACGCT GCTTAAGTAG 
               
               
                   
               
               
                 1901 
                 AACGTGCCCG AATGGAGCTA CATCGTGGAG AAGGCCAACC CAGCCAACGA 
               
               
                   
                 TTGCACGGGC TTACCTCGAT GTAGCACCTC TTCCGGTTGG GTCGGTTGCT 
               
               
                   
               
               
                 1951 
                 CCTGTGCTAC CCAGGGAACC TGAACGACTA CGAAGAACTG AAACACCTGC 
               
               
                   
                 GGACACGATG GGTCCCTTGG ACTTGCTGAT GCTTCTTGAC TTTGTGGACG 
               
               
                   
               
               
                 2001 
                 TGAGCAGAAT CAACCACTTT GAGAAAATCC AGATCATCCC CAAAAGCAGC 
               
               
                   
                 ACTCGTCTTA GTTGGTGAAA CTCTTTTAGG TCTAGTAGGG GTTTTCGTCG 
               
               
                   
               
               
                 2051 
                 TGGTCCGATC ACGAAGCCAG CAGCGGAGTG AGCAGCGCCT GCCCATACCA 
               
               
                   
                 ACCAGGCTAG TGCTTCGGTC GTCGCCTCAC TCGTCGCGGA CGGGTATGGT 
               
               
                   
               
               
                 2101 
                 GGGAAAGTCC AGCTTTTTTA GAAACGTGGT GTGGCTGATC AAAAAGAACA 
               
               
                   
                 CCCTTTCAGG TCGAAAAAAT CTTTGCACCA CACCGACTAG TTTTTCTTGT 
               
               
                   
               
               
                 2151 
                 GCGCCTACCC AACAATCAAG AGAAGCTACA ACAACACCAA CCAGGAAGAT 
               
               
                   
                 CGCGGATGGG TTGTTAGTTC TCTTCGATGT TGTTGTGGTT GGTCCTTCTA 
               
               
                   
               
               
                 2201 
                 CTGCTGGTGC TGTGGGGGAT CCACCACCCT AACGATGCCG CCGAGCAGAC 
               
               
                   
                 GACGACCACG ACACCCCCTA GGTGGTGGGA TTGCTACGGC GGCTCGTCTG 
               
               
                   
               
               
                 2251 
                 AAGGCTGTAC CAGAACCCAA CCACCTACAT CTCCGTGGGG ACAAGCACAC 
               
               
                   
                 TTCCGACATG GTCTTGGGTT GGTGGATGTA GAGGCACCCC TGTTCGTGTG 
               
               
                   
               
               
                 2301 
                 TGAACCAGAG ACTGGTGCCA AAAATCGCCA TCAGATCCAA AGTGAACGGG 
               
               
                   
                 ACTTGGTCTC TGACCACGGT TTTTAGCGGT AGTCTAGGTT TCACTTGCCC 
               
               
                   
               
               
                 2351 
                 CAGAGCGGAA GAATGGAGTT CTTCTGGACA ATCCTGAAAC CCAACGATGC 
               
               
                   
                 GTCTCGCCTT CTTACCTCAA GAAGACCTGT TAGGACTTTG GGTTGCTACG 
               
               
                   
               
               
                 2401 
                 CATCAACTTC GAGAGCAACG GAAACTTCAT CGCCCCAGAA TACGCCTACA 
               
               
                   
                 GTAGTTGAAG CTCTCGTTGC CTTTGAAGTA GCGGGGTCTT ATGCGGATGT 
               
               
                   
               
               
                 2451 
                 AAATCGTGAA GAAAGGGGAC AGCGCCATCA TGAAAAGCGA ACTGGAATAC 
               
               
                   
                 TTTAGCACTT CTTTCCCCTG TCGCGGTAGT ACTTTTCGCT TGACCTTATG 
               
               
                   
               
               
                 2501 
                 GGCAACTGCA ACACCAAGTG CCAGACCCCA ATGGGGGCCA TCAACAGCAG 
               
               
                   
                 CCGTTGACGT TGTGGTTCAC GGTCTGGGGT TACCCCCGGT AGTTGTCGTC 
               
               
                   
               
               
                 2551 
                 CATGCCATTC CACAACATCC ACCCTCTGAC CATCGGGGAA TGCCCCAAAT 
               
               
                   
                 GTACGGTAAG GTGTTGTAGG TGGGAGACTG GTAGCCCCTT ACGGGGTTTA 
               
               
                   
               
               
                 2601 
                 ACGTGAAAAG CAACAGACTG GTGCTGGCCA CCGGGCTGAG AAACAGCCCT 
               
               
                   
                 TGCACTTTTC GTTGTCTGAC CACGACCGGT GGCCCGACTC TTTGTCGGGA 
               
               
                   
               
               
                 2651 
                 CAGAGAGAGA CCAGAGGACT GTTTGGAGCC ATCGCCGGCT TTATCGAGGG 
               
               
                   
                 GTCTCTCTCT GGTCTCCTGA CAAACCTCGG TAGCGGCCGA AATAGCTCCC 
               
               
                   
               
               
                 2701 
                 AGGATGGCAG GGAATGGTGG ATGGCTGGTA CGGATACCAC CACAGCAACG 
               
               
                   
                 TCCTACCGTC CCTTACCACC TACCGACCAT GCCTATGGTG GTGTCGTTGC 
               
               
                   
               
               
                 2751 
                 AGCAGGGGAG CGGATACGCC GCCGACAAAG AATCCACCCA GAAGGCCATC 
               
               
                   
                 TCGTCCCCTC GCCTATGCGG CGGCTGTTTC TTAGGTGGGT CTTCCGGTAG 
               
               
                   
               
               
                 2801 
                 GACGGCGTGA CCAACAAAGT GAACAGCATC ATCGACAAAA TGAACACCCA 
               
               
                   
                 CTGCCGCACT GGTTGTTTCA CTTGTCGTAG TAGCTGTTTT ACTTGTGGGT 
               
               
                   
               
               
                 2851 
                 GTTTGAGGCC GTGGGAAGGG AGTTTAACAA CCTGGAAAGG AGAATCGAGA 
               
               
                   
                 CAAACTCCGG CACCCTTCCC TCAAATTGTT GGACCTTTCC TCTTAGCTCT 
               
               
                   
               
               
                 2901 
                 ACCTGAACAA GAAGATGGAG GACGGATTCC TGGATGTGTG GACCTACAAC 
               
               
                   
                 TGGACTTGTT CTTCTACCTC CTGCCTAAGG ACCTACACAC CTGGATGTTG 
               
               
                   
               
               
                 2951 
                 GCCGAACTGC TGGTGCTGAT GGAAAACGAG AGAACCCTGG ACTTTCACGA 
               
               
                   
                 CGGCTTGACG ACCACGACTA CCTTTTGCTC TCTTGGGACC TGAAAGTGCT 
               
               
                   
               
               
                 3001 
                 CAGCAACGTG AAGAACCTGT ACGACAAAGT GAGGCTGCAG CTGAGGGATA 
               
               
                   
                 GTCGTTGCAC TTCTTGGACA TGCTGTTTCA CTCCGACGTC GACTCCCTAT 
               
               
                   
               
               
                 3051 
                 ACGCCAAGGA GCTGGGCAAC GGCTGCTTCG AGTTCTACCA CAAATGCGAT 
               
               
                   
                 TGCGGTTCCT CGACCCGTTG CCGACGAAGC TCAAGATGGT GTTTACGCTA 
               
               
                   
               
               
                 3101 
                 AACGAATGCA TGGAAAGCAT CAGAAACGGA ACCTACAACT ACCCCCAGTA 
               
               
                   
                 TTGCTTACGT ACCTTTCGTA GTCTTTGCCT TGGATGTTGA TGGGGGTCAT 
               
               
                   
               
               
                 3151 
                 CAGCGAAGAA GCCAGACTGA AAAGAGAAGA AATCTCCGGA GTGAAACTGG 
               
               
                   
                 GTCGCTTCTT CGGTCTGACT TTTCTCTTCT TTAGAGGCCT CACTTTGACC 
               
               
                   
               
               
                 3201 
                 AATCCATCGG AACCTACCAG ATCCTGAGCA TCTACAGCAC AGTGGCCTCC 
               
               
                   
                 TTAGGTAGCC TTGGATGGTC TAGGACTCGT AGATGTCGTG TCACCGGAGG 
               
               
                   
               
               
                 3251 
                 TCCCTGGCCC TGGCCATCAT GATGGCCGGA CTGAGCCTGT GGATGTGCTC 
               
               
                   
                 AGGGACCGGG ACCGGTAGTA CTACCGGCCT GACTCGGACA CCTACACGAG 
               
               
                   
               
               
                 3301 
                 CAACGGAAGC CTGCAGTGCA GAATCTGCAT CTGACTCGAG TTTTTATTGA 
               
               
                   
                 GTTGCCTTCG GACGTCACGT CTTAGACGTA GACTGAGCTC AAAAATAACT 
               
               
                   
               
               
                 3351 
                 CTAGTTAATC ATAAGATAAA TAATATACAG CATTGTAACC ATCGTCATCC 
               
               
                   
                 GATCAATTAG TATTCTATTT ATTATATGTC GTAACATTGG TAGCAGTAGG 
               
               
                   
               
               
                 3401 
                 GTTATACGGG GAATAATATT ACCATACAGT ATTATTAAAT TTTCTTACGA 
               
               
                   
                 CAATATGCCC CTTATTATAA TGGTATGTCA TAATAATTTA AAAGAATGCT 
               
               
                   
               
               
                 3451 
                 AGAATATAGA TCGGTATTTA TCGTTAGTTT ATTTTACATT TATTAATTAA 
               
               
                   
                 TCTTATATCT AGCCATAAAT AGCAATCAAA TAAAATGTAA ATAATTAATT 
               
               
                   
               
               
                 3501 
                 ACATGTCTAC TATTACCTGT TATGGAAATG ACAAATTTAG TTATATAATT 
               
               
                   
                 TGTACAGATG ATAATGGACA ATACCTTTAC TGTTTAAATC AATATATTAA 
               
               
                   
               
               
                 3551 
                 TATGATAAAA TTAAGATAAT AATAATGAAA TCAAATAATT ATGTAAATGC 
               
               
                   
                 ATACTATTTT AATTCTATTA TTATTACTTT AGTTTATTAA TACATTTACG 
               
               
                   
               
               
                 3601 
                 TACTAGATTA TGTGAATTAC GAGGAAGAAA GTTTACGAAC TGGAAAAAAT 
               
               
                   
                 ATGATCTAAT ACACTTAATG CTCCTTCTTT CAAATGCTTG ACCTTTTTTA 
               
               
                   
               
               
                 3651 
                 TAAGTGAATC TAAAATATTA GTCGATAATG TAAAAAAAAT AAATGATAAA 
               
               
                   
                 ATTCACTTAG ATTTTATAAT CAGCTATTAC ATTTTTTTTA TTTACTATTT 
               
               
                   
               
               
                 3701 
                 ACTAACCAGT TAAAAACGGA TATGATTATA TACGTTAAGG ATATTGATCA 
               
               
                   
                 TGATTGGTCA ATTTTTGCCT ATACTAATAT ATGCAATTCC TATAACTAGT 
               
               
                   
               
               
                 3751 
                 TAAAGGAAGA GATACTTGCG GTTACTATGT ACACCAAGAT CTGGTATCTT 
               
               
                   
                 ATTTCCTTCT CTATGAACGC CAATGATACA TGTGGTTCTA GACCATAGAA 
               
               
                   
               
               
                 3801 
                 CTATATCAAA TTGGATATCT CCGTTATTCG CCGTTAAGGT AAATAAAATT 
               
               
                   
                 GATATAGTTT AACCTATAGA GGCAATAAGC GGCAATTCCA TTTATTTTAA 
               
               
                   
               
               
                 3851 
                 ATTAACTATT ATATATGTAA TGAATATGAT ATACGACTTA GCGAAATGGA 
               
               
                   
                 TAATTGATAA TATATACATT ACTTATACTA TATGCTGAAT CGCTTTACCT 
               
               
                   
               
               
                 3901 
                 ATCTGATATG ACAGAAGTAA TAGATGTAGT TGATAAATTA GTAGGAGGAT 
               
               
                   
                 TAGACTATAC TGTCTTCATT ATCTACATCA ACTATTTAAT CATCCTCCTA 
               
               
                   
               
               
                 3951 
                 ACAATGATGA AATAGCAGAA ATAATATATT TGTTTAATAA ATTTATAGAA 
               
               
                   
                 TGTTACTACT TTATCGTCTT TATTATATAA ACAAATTATT TAAATATCTT 
               
               
                   
               
               
                 4001 
                 AAATATATTG CTAACATATC GTTATCAACT GAATTATCTA GTATATTAAA 
               
               
                   
                 TTTATATAAC GATTGTATAG CAATAGTTGA CTTAATAGAT CATATAATTT 
               
               
                   
               
               
                 4051 
                 TAATTTTATA AATTTTAATA AAAAATACAA TAACGACATA AAAGATATTA 
               
               
                   
                 ATTAAAATAT TTAAAATTAT TTTTTATGTT ATTGCTGTAT TTTCTATAAT 
               
               
                   
               
               
                 4101 
                 AATCTTTAAT TCTTGATCTG AAAAACACAT CTATAAAACT AGATAAAAAG 
               
               
                   
                 TTAGAAATTA AGAACTAGAC TTTTTGTGTA GATATTTTGA TCTATTTTTC 
               
               
                   
               
               
                 4151 
                 TTATTCGATA AAGATAATAA TGAATCGAAC GATGAAAAAT TGGAAACAGA 
               
               
                   
                 AATAAGCTAT TTCTATTATT ACTTAGCTTG CTACTTTTTA ACCTTTGTCT 
               
               
                   
               
               
                 4201 
                 AGTTGATAAG CTAATTTTTT TCATCTAAAT AGTATTATTT TATTGAAGTA 
               
               
                   
                 TCAACTATTC GATTAAAAAA AGTAGATTTA TCATAATAAA ATAACTTCAT 
               
               
                   
               
               
                 4251 
                 CGAAGTTTTA CGTTAGATAA ATAATAAAGG TCGATTTTTA TTTTGTTAAA 
               
               
                   
                 GCTTCAAAAT GCAATCTATT TATTATTTCC AGCTAAAAAT AAAACAATTT 
               
               
                   
               
               
                 4301 
                 TATCAAATAT GTCATTATCT GATAAAGATA CAAAAACACA CGGTGATTAT 
               
               
                   
                 ATAGTTTATA CAGTAATAGA CTATTTCTAT GTTTTTGTGT GCCACTAATA 
               
               
                   
               
               
                 4351 
                 CAACCATCTA ACGAACAGAT ATTACAAAAA ATACGTCGGA CTATGGAAAA 
               
               
                   
                 GTTGGTAGAT TGCTTGTCTA TAATGTTTTT TATGCAGCCT GATACCTTTT 
               
               
                   
               
               
                 4401 
                 CGAAGCTGAT AGCCTCAATA GAAGAAGCAT TAAAGAAATT GTTGTAGATG 
               
               
                   
                 GCTTCGACTA TCGGAGTTAT CTTCTTCGTA ATTTCTTTAA CAACATCTAC 
               
               
                   
               
               
                 4451 
                 TTATGAAGAA TTGGGATCAT CCTCTCAACG AAGAAATAGA TAAAGTTCTA 
               
               
                   
                 AATACTTCTT AACCCTAGTA GGAGAGTTGC TTCTTTATCT ATTTCAAGAT 
               
               
                   
               
               
                 4501 
                 AACTGGAAAA ATGATACATT AAACGATTTA GATCATCTAA ATACAGATGA 
               
               
                   
                 TTGACCTTTT TACTATGTAA TTTGCTAAAT CTAGTAGATT TATGTCTACT 
               
               
                   
               
               
                 4551 
                 TAATATTAAG GAAATCATAC AATGTCTGAT TAGAGAATTT GCGTTTAAAA 
               
               
                   
                 ATTATAATTC CTTTAGTATG TTACAGACTA ATCTCTTAAA CGCAAATTTT 
               
               
                   
               
               
                 4601 
                 AGATCAATTC TATTATGTAT AGTTATGCTA TGGTAAAACT CAATTCAGAT 
               
               
                   
                 TCTAGTTAAG ATAATACATA TCAATACGAT ACCATTTTGA GTTAAGTCTA 
               
               
                   
               
               
                 4651 
                 AACGAAACAT TGAAAGATAA AATTAAGGAT TATTTTATAG AAACTATTCT 
               
               
                   
                 TTGCTTTGTA ACTTTCTATT TTAATTCCTA ATAAAATATC TTTGATAAGA 
               
               
                   
               
               
                 4701 
                 TAAAGACAAA CGTGGTTATA AACAAAAGCC ATTACCC 
               
               
                   
                 ATTTCTGTTT GCACCAATAT TTGTTTTCGG TAATGGG 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 15 
                   
               
               
                 LENGTH: 1692 
                   
               
               
                   
               
               
                 OTHER INFORMATION: nucleotide sequence of wild type H5 HA without cleavage 
               
               
                 sequence from plasmid pCRScript/HACK/Indonesia/03(modified)avipox 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 (1) 
                 ATGGAGAAAATCGTGCTGCTGCTGGCCATCGTGAGCCTGGTGAAAAGCGATCAGATCTGCATCGGCTACC 
                   
               
               
                   
               
               
                 (71) 
                 ACGCCAACAACAGCACAGAGCAAGTGGACACAATCATGGAAAAGAACGTGACCGTGACACACGCCCAGGA 
               
               
                   
               
               
                 (141) 
                 CATCCTGGAAAAGACACACAACGGGAAGCTGTGCGATCTGGATGGAGTGAAGCCTCTGATCCTGAGAGAT 
               
               
                   
               
               
                 (211) 
                 TGCAGCGTGGCCGGATGGCTGCTGGGGAACCCAATGTGCGACGAATTCATCAACGTGCCCGAATGGAGCT 
               
               
                   
               
               
                 (281) 
                 ACATCGTGGAGAAGGCCAACCCAGCCAACGACCTGTGCTACCCAGGGAACCTGAACGACTACGAAGAACT 
               
               
                   
               
               
                 (351) 
                 GAAACACCTGCTGAGCAGAATCAACCACTTTGAGAAAATCCAGATCATCCCCAAAAGCAGCTGGTCCGAT 
               
               
                   
               
               
                 (421) 
                 CACGAAGCCAGCAGCGGAGTGAGCAGCGCCTGCCCATACCAGGGAAAGTCCAGCTTTTTTAGAAACGTGG 
               
               
                   
               
               
                 (491) 
                 TGTGGCTGATCAAAAAGAACAGCGCCTACCCAACAATCAAGAGAAGCTACAACAACACCAACCAGGAAGA 
               
               
                   
               
               
                 (561) 
                 TCTGCTGGTGCTGTGGGGGATCCACCACCCTAACGATGCCGCCGAGCAGACAAGGCTGTACCAGAACCCA 
               
               
                   
               
               
                 (631) 
                 ACCACCTACATCTCCGTGGGGACAAGCACACTGAACCAGAGACTGGTGCCAAAAATCGCCATCAGATCCA 
               
               
                   
               
               
                 (701) 
                 AAGTGAACGGGCAGAGCGGAAGAATGGAGTTCTTCTGGACAATCCTGAAACCCAACGATGCCATCAACTT 
               
               
                   
               
               
                 (771) 
                 CGAGAGCAACGGAAACTTCATCGCCCCAGAATACGCCTACAAAATCGTGAAGAAAGGGGACAGCGCCATC 
               
               
                   
               
               
                 (841) 
                 ATGAAAAGCGAACTGGAATACGGCAACTGCAACACCAAGTGCCAGACCCCAATGGGGGCCATCAACAGCA 
               
               
                   
               
               
                 (911) 
                 GCATGCCATTCCACAACATCCACCCTCTGACCATCGGGGAATGCCCCAAATACGTGAAAAGCAACAGACT 
               
               
                   
               
               
                 (981) 
                 GGTGCTGGCCACCGGGCTGAGAAACAGCCCTCAGAGAGAGACCAGAGGACTGTTTGGAGCCATCGCCGGC 
               
               
                   
               
               
                 (1051) 
                 TTTATCGAGGGAGGATGGCAGGGAATGGTGGATGGCTGGTACGGATACCACCACAGCAACGAGCAGGGGA 
               
               
                   
               
               
                 (1121) 
                 GCGGATACGCCGCCGACAAAGAATCCACCCAGAAGGCCATCGACGGCGTGACCAACAAAGTGAACAGCAT 
               
               
                   
               
               
                 (1191) 
                 CATCGACAAAATGAACACCCAGTTTGAGGCCGTGGGAAGGGAGTTTAACAACCTGGAAAGGAGAATCGAG 
               
               
                   
               
               
                 (1261) 
                 AACCTGAACAAGAAGATGGAGGACGGATTCCTGGATGTGTGGACCTACAACGCCGAACTGCTGGTGCTGA 
               
               
                   
               
               
                 (1331) 
                 TGGAAAACGAGAGAACCCTGGACTTTCACGACAGCAACGTGAAGAACCTGTACGACAAAGTGAGGCTGCA 
               
               
                   
               
               
                 (1401) 
                 GCTGAGGGATAACGCCAAGGAGCTGGGCAACGGCTGCTTCGAGTTCTACCACAAATGCGATAACGAATGC 
               
               
                   
               
               
                 (1471) 
                 ATGGAAAGCATCAGAAACGGAACCTACAACTACCCCCAGTACAGCGAAGAAGCCAGACTGAAAAGAGAAG 
               
               
                   
               
               
                 (1541) 
                 AAATCTCCGGAGTGAAACTGGAATCCATCGGAACCTACCAGATCCTGAGCATCTACAGCACAGTGGCCTC 
               
               
                   
               
               
                 (1611) 
                 CTCCCTGGCCCTGGCCATCATGATGGCCGGACTGAGCCTGTGGATGTGCTCCAACGGAAGCCTGCAGTGC 
               
               
                   
               
               
                 (1681) 
                 AGAATCTGCATC 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 16 
                   
               
               
                 LENGTH: 564 
                   
               
               
                   
               
               
                 OTHER INFORMATION: amino acid sequence of wild type H5 HA without cleavage 
               
               
                 sequence from plasmid pCRScript/HACK/Indonesia/03(modified)avipox 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 (1) 
                 MEKIVLLLAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILE 
                   
               
               
                   
               
               
                 (51) 
                 KTHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKAN 
               
               
                   
               
               
                 (101) 
                 PANDLCYPGNLNDYEELKHLLSRINHFEKIQIIPKSSWSDHEASSGVSSA 
               
               
                   
               
               
                 (151) 
                 CPYQGKSSFFRNVVWLIKKNSAYPTIKRSYNNTNQEDLLVLWGIHHPNDA 
               
               
                   
               
               
                 (201) 
                 AEQTRLYQNPTTYISVGTSTLNQRLVPKIAIRSKVNGQSGRMEFFWTILK 
               
               
                   
               
               
                 (251) 
                 PNDAINFESNGNFIAPEYAYKIVKKGDSAIMKSELEYGNCNTKCQTPMGA 
               
               
                   
               
               
                 (301) 
                 INSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSPQRETRGLFG 
               
               
                   
               
               
                 (347) 
                 AIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDGVTNKVNS 
               
               
                   
               
               
                 (397) 
                 IIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMEN 
               
               
                   
               
               
                 (447) 
                 ERTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESIRN 
               
               
                   
               
               
                 (497) 
                 GTYNYPQYSEEARLKREEISGVKLESIGTYQILSIYSTVASSLALAIMMA 
               
               
                   
               
               
                 (547) 
                 GLSLWMCSNGSLQCRICI 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 17 
                   
               
               
                 LENGTH: 564 
                   
               
               
                   
               
               
                 OTHER INFORMATION: Predicted amino acid sequence of H5 HA without cleavage 
               
               
                 sequence 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 MEKIVLLLAI VSLVKSDQIC IGYHANNSTE QVDTIMEKNV TVTHAQDILE 
                   
               
               
                   
               
               
                 51 
                 KTHNGKLCDL DGVKPLILRD CSVAGWLLGN PMCDEFINVP EWSYIVEKAN 
               
               
                   
               
               
                 101 
                 PANDLCYPGN LNDYEELKHL LSRINHFEKI QIIPKSSWSD HEASSGVSSA 
               
               
                   
               
               
                 151 
                 CPYQGKSSFF RNVVWLIKKN SAYPTIKRSY NNTNQEDLLV LWGIHHPNDA 
               
               
                   
               
               
                 201 
                 AEQTRLYQNP TTYISVGTST LNQRLVPKIA IRSKVNGQSG RMEFFWTILK 
               
               
                   
               
               
                 251 
                 PNDAINFESN GNFIAPEYAY KIVKKGDSAI MKSELEYGNC NTKCQTPMGA 
               
               
                   
               
               
                 301 
                 INSSMPFHNI HPLTIGECPK YVKSNRLVLA TGLRNSPQRE TRGLFGAIAG 
               
               
                   
               
               
                 351 
                 FIEGGWQGMV DGWYGYHHSN EQGSGYAADK ESTQKAIDGV TNKVNSIIDK 
               
               
                   
               
               
                 401 
                 MNTQFEAVGR EFNNLERRIE NLNKKMEDGF LDVWTYNAEL LVLMENERTL 
               
               
                   
               
               
                 451 
                 DFHDSNVKNL YDKVRLQLRD NAKELGNGCF EFYHKCDNEC MESIRNGTYN 
               
               
                   
               
               
                 501 
                 YPQYSEEARL KREEISGVKL ESIGTYQILS IYSTVASSLA LAIMMAGLSL 
               
               
                   
               
               
                 551 
                 WMCSNGSLQC RICI* 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 18 
                   
               
               
                 LENGTH: 3956 
                   
               
               
                   
               
               
                 ORGANISM: Artificial Sequence 
               
               
                   
               
               
                 OTHER INFORMATION: Nucleotide sequence of C5 right arm (43-1578), H6 promoter 
               
               
                 (1675-1799) AIV synthetic H5 HA (1800-3494), and C5 left arm (3529-3932) in 
               
               
                 plasmid 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 GGAAACAGCT ATGACCATGA TTACGAATTG CGGCCGCAAT TCTGAATGTT 
                   
               
               
                   
                 CCTTTGTCGA TACTGGTACT AATGCTTAAC GCCGGCGTTA AGACTTACAA 
               
               
                   
               
               
                 51 
                 AAATGTTATA CTTTGGATGA AGCTATAAAT ATGCATTGGA AAAATAATCC 
               
               
                   
                 TTTACAATAT GAAACCTACT TCGATATTTA TACGTAACCT TTTTATTAGG 
               
               
                   
               
               
                 101 
                 ATTTAAAGAA AGGATTCAAA TACTACAAAA CCTAAGCGAT AATATGTTAA 
               
               
                   
                 TAAATTTCTT TCCTAAGTTT ATGATGTTTT GGATTCGCTA TTATACAATT 
               
               
                   
               
               
                 151 
                 CTAAGCTTAT TCTTAACGAC GCTTTAAATA TACACAAATA AACATAATTT 
               
               
                   
                 GATTCGAATA AGAATTGCTG CGAAATTTAT ATGTGTTTAT TTGTATTAAA 
               
               
                   
               
               
                 201 
                 TTGTATAACC TAACAAATAA CTAAAACATA AAAATAATAA AAGGAAATGT 
               
               
                   
                 AACATATTGG ATTGTTTATT GATTTTGTAT TTTTATTATT TTCCTTTACA 
               
               
                   
               
               
                 251 
                 AATATCGTAA TTATTTTACT CAGGAATGGG GTTAAATATT TATATCACGT 
               
               
                   
                 TTATAGCATT AATAAAATGA GTCCTTACCC CAATTTATAA ATATAGTGCA 
               
               
                   
               
               
                 301 
                 GTATATCTAT ACTGTTATCG TATACTCTTT ACAATTACTA TTACGAATAT 
               
               
                   
                 CATATAGATA TGACAATAGC ATATGAGAAA TGTTAATGAT AATGCTTATA 
               
               
                   
                                                         7927.DC 
               
               
                   
               
               
                 351 
                 GCAAGAGATA ATAAGATTAC GTATTTAAGA GAATCTTGTC ATGATAATTG 
               
               
                   
                 CGTTCTCTAT TATTCTAATG CATAAATTCT CTTAGAACAG TACTATTAAC 
               
               
                   
                 7927.DC 
               
               
                   
               
               
                 401 
                 GGTACGACAT AGTGATAAAT GCTATTTCGC ATCGTTACAT AAAGTCAGTT 
               
               
                   
                 CCATGCTGTA TCACTATTTA CGATAAAGCG TAGCAATGTA TTTCAGTCAA 
               
               
                   
               
               
                 451 
                 GGAAAGATGG ATTTGACAGA TGTAACTTAA TAGGTGCAAA AATGTTAAAT 
               
               
                   
                 CCTTTCTACC TAAACTGTCT ACATTGAATT ATCCACGTTT TTACAATTTA 
               
               
                   
                               7696.CXL 
               
               
                   
               
               
                 501 
                 AACAGCATTC TATCGGAAGA TAGGATACCA GTTATATTAT ACAAAAATCA 
               
               
                   
                 TTGTCGTAAG ATAGCCTTCT ATCCTATGGT CAATATAATA TGTTTTTAGT 
               
               
                   
               
               
                 551 
                 CTGGTTGGAT AAAACAGATT CTGCAATATT CGTAAAAGAT GAAGATTACT 
               
               
                   
                 GACCAACCTA TTTTGTCTAA GACGTTATAA GCATTTTCTA CTTCTAATGA 
               
               
                   
               
               
                 601 
                 GCGAATTTGT AAACTATGAC AATAAAAAGC CATTTATCTC AACGACATCG 
               
               
                   
                 CGCTTAAACA TTTGATACTG TTATTTTTCG GTAAATAGAG TTGCTGTAGC 
               
               
                   
               
               
                 651 
                 TGTAATTCTT CCATGTTTTA TGTATGTGTT TCAGATATTA TGAGATTACT 
               
               
                   
                 ACATTAAGAA GGTACAAAAT ACATACACAA AGTCTATAAT ACTCTAATGA 
               
               
                   
               
               
                 701 
                 ATAAACTTTT TGTATACTTA TATTCCGTAA ACTATATTAA TCATGAAGAA 
               
               
                   
                 TATTTGAAAA ACATATGAAT ATAAGGCATT TGATATAATT AGTACTTCTT 
               
               
                   
               
               
                 751 
                 AATGAAAAAG TATAGAAGCT GTTCACGAGC GGTTGTTGAA AACAACAAAA 
               
               
                   
                 TTACTTTTTC ATATCTTCGA CAAGTGCTCG CCAACAACTT TTGTTGTTTT 
               
               
                   
                                         7926.DC 
               
               
                   
               
               
                 801 
                 TTATACATTC AAGATGGCTT ACATATACGT CTGTGAGGCT ATCATGGATA 
               
               
                   
                 AATATGTAAG TTCTACCGAA TGTATATGCA GACACTCCGA TAGTACCTAT 
               
               
                   
               
               
                 851 
                 ATGACAATGC ATCTCTAAAT AGGTTTTTGG ACAATGGATT CGACCCTAAC 
               
               
                   
                 TACTGTTACG TAGAGATTTA TCCAAAAACC TGTTACCTAA GCTGGGATTG 
               
               
                   
               
               
                 901 
                 ACGGAATATG GTACTCTACA ATCTCCTCTT GAAATGGCTG TAATGTTCAA 
               
               
                   
                 TGCCTTATAC CATGAGATGT TAGAGGAGAA CTTTACCGAC ATTACAAGTT 
               
               
                   
               
               
                 951 
                 GAATACCGAG GCTATAAAAA TCTTGATGAG GTATGGAGCT AAACCTGTAG 
               
               
                   
                 CTTATGGCTC CGATATTTTT AGAACTACTC CATACCTCGA TTTGGACATC 
               
               
                   
                                 7697.CXL 
               
               
                   
               
               
                 1001 
                 TTACTGAATG CACAACTTCT TGTCTGCATG ATGCGGTGTT GAGAGACGAC 
               
               
                   
                 AATGACTTAC GTGTTGAAGA ACAGACGTAC TACGCCACAA CTCTCTGCTG 
               
               
                   
               
               
                 1051 
                 TACAAAATAG TGAAAGATCT GTTGAAGAAT AACTATGTAA ACAATGTTCT 
               
               
                   
                 ATGTTTTATC ACTTTCTAGA CAACTTCTTA TTGATACATT TGTTACAAGA 
               
               
                   
               
               
                 1101 
                 TTACAGCGGA GGCTTTACTC CTTTGTGTTT GGCAGCTTAC CTTAACAAAG 
               
               
                   
                 AATGTCGCCT CCGAAATGAG GAAACACAAA CCGTCGAATG GAATTGTTTC 
               
               
                   
               
               
                 1151 
                 TTAATTTGGT TAAACTTCTA TTGGCTCATT CGGCGGATGT AGATATTTCA 
               
               
                   
                 AATTAAACCA ATTTGAAGAT AACCGAGTAA GCCGCCTACA TCTATAAAGT 
               
               
                   
               
               
                 1201 
                 AACACGGATC GGTTAACTCC TCTACATATA GCCGTATCAA ATAAAAATTT 
               
               
                   
                 TTGTGCCTAG CCAATTGAGG AGATGTATAT CGGCATAGTT TATTTTTAAA 
               
               
                   
                                     7925.DC 
               
               
                   
               
               
                 1251 
                 AACAATGGTT AAACTTCTAT TGAACAAAGG TGCTGATACT GACTTGCTGG 
               
               
                   
                 TTGTTACCAA TTTGAAGATA ACTTGTTTCC ACGACTATGA CTGAACGACC 
               
               
                   
               
               
                 1301 
                 ATAACATGGG ACGTACTCCT TTAATGATCG CTGTACAATC TGGAAATATT 
               
               
                   
                 TATTGTACCC TGCATGAGGA AATTACTAGC GACATGTTAG ACCTTTATAA 
               
               
                   
               
               
                 1351 
                 GAAATATGTA GCACACTACT TAAAAAAAAT AAAATGTCCA GAACTGGGAA 
               
               
                   
                 CTTTATACAT CGTGTGATGA ATTTTTTTTA TTTTACAGGT CTTGACCCTT 
               
               
                   
               
               
                 1401 
                 AAATTGATCT TGCCAGCTGT AATTCATGGT AGAAAAGAAG TGCTCAGGCT 
               
               
                   
                 TTTAACTAGA ACGGTCGACA TTAAGTACCA TCTTTTCTTC ACGAGTCCGA 
               
               
                   
               
               
                 1451 
                 ACTTTTCAAC AAAGGAGCAG ATGTAAACTA CATCTTTGAA AGAAATGGAA 
               
               
                   
                 TGAAAAGTTG TTTCCTCGTC TACATTTGAT GTAGAAACTT TCTTTACCTT 
               
               
                   
                                                            7792.SL 
               
               
                   
               
               
                 1501 
                 AATCATATAC TGTTTTGGAA TTGATTAAAG AAAGTTACTC TGAGACACAA 
               
               
                   
                 TTAGTATATG ACAAAACCTT AACTAATTTC TTTCAATGAG ACTCTGTGTT 
               
               
                   
                   7792.SL 
               
               
                   
               
               
                 1551 
                 AAGAGGTAGC TGAAGTGGTA CTCTCAAAGG TACGTGACTA ATTAGCTATA 
               
               
                   
                 TTCTCCATCG ACTTCACCAT GAGAGTTTCC ATGCACTGAT TAATCGATAT 
               
               
                   
               
               
                 1601 
                 AAAAGGATCC GGGTTAATTA ATTAGTCATC AGGCAGGGCG AGAACGAGAC 
               
               
                   
                 TTTTCCTAGG CCCAATTAAT TAATCAGTAG TCCGTCCCGC TCTTGCTCTG 
               
               
                   
                                               H6p 
               
               
                   
               
               
                 1651 
                 TATCTGCTCG TTAATTAATT AGAGCTTCTT TATTCTATAC TTAAAAAGTG 
               
               
                   
                 ATAGACGAGC AATTAATTAA TCTCGAAGAA ATAAGATATG AATTTTTCAC 
               
               
                   
               
               
                 1701 
                 AAAATAAATA CAAAGGTTCT TGAGGGTTGT GTTAAATTGA AAGCGAGAAA 
               
               
                   
                 TTTTATTTAT GTTTCCAAGA ACTCCCAACA CAATTTAACT TTCGCTCTTT 
               
               
                   
                                                               H5 HA  M • 
               
               
                   
               
               
                 1751 
                 TAATCATAAA TTATTTCATT ATCGCGATAT CCGTTAAGTT TGTATCGTAA 
               
               
                   
                 ATTAGTATTT AATAAAGTAA TAGCGCTATA GGCAATTCAA ACATAGCATT 
               
               
                   
                       11524JY 
               
               
                   
               
               
                   
                 .. E  K  I   V  L  L   L  A  I  V   S  L  V   K  S  D 
               
               
                 1801 
                 TGGAGAAAAT CGTGCTGCTG CTGGCCATCG TGAGCCTGGT GAAAAGCGAT 
               
               
                   
                 ACCTCTTTTA GCACGACGAC GACCGGTAGC ACTCGGACCA CTTTTCGCTA 
               
               
                   
                       11525JY 
               
               
                   
               
               
                   
                  Q  I  C  I   G  Y  H   A  N  N   S  T  E  Q   V  D  T • 
               
               
                 1851 
                 CAGATCTGCA TCGGCTACCA CGCCAACAAC AGCACAGAGC AAGTGGACAC 
               
               
                   
                 GTCTAGACGT AGCCGATGGT GCGGTTGTTG TCGTGTCTCG TTCACCTGTG 
               
               
                   
               
               
                   
                 . I  M  E   K  N  V  T   V  T  H   A  Q  D   I  L  E  K • 
               
               
                 1901 
                 AATCATGGAA AAGAACGTGA CCGTGACACA CGCCCAGGAC ATCCTGGAAA 
               
               
                   
                 TTAGTACCTT TTCTTGCACT GGCACTGTGT GCGGGTCCTG TAGGACCTTT 
               
               
                   
               
               
                   
                 .. T  H  N   G  K  L   C  D  L  D   G  V  K   P  L  I 
               
               
                 1951 
                 AGACACACAA CGGGAAGCTG TGCGATCTGG ATGGAGTGAA GCCTCTGATC 
               
               
                   
                 TCTGTGTGTT GCCCTTCGAC ACGCTAGACC TACCTCACTT CGGAGACTAG 
               
               
                   
               
               
                   
                  L  R  D  C   S  V  A   G  W  L   L  G  N  P   M  C  D • 
               
               
                 2001 
                 CTGAGAGATT GCAGCGTGGC CGGATGGCTG CTGGGGAACC CAATGTGCGA 
               
               
                   
                 GACTCTCTAA CGTCGCACCG GCCTACCGAC GACCCCTTGG GTTACACGCT 
               
               
                   
               
               
                   
                 . E  F  I   N  V  P  E   W  S  Y   I  V  E   K  A  N  P • 
               
               
                 2051 
                 CGAATTCATC AACGTGCCCG AATGGAGCTA CATCGTGGAG AAGGCCAACC 
               
               
                   
                 GCTTAAGTAG TTGCACGGGC TTACCTCGAT GTAGCACCTC TTCCGGTTGG 
               
               
                   
               
               
                   
                 .. A  N  D   L  C  Y   P  G  N  L   N  D  Y   E  E  L 
               
               
                 2101 
                 CAGCCAACGA CCTGTGCTAC CCAGGGAACC TGAACGACTA CGAAGAACTG 
               
               
                   
                 GTCGGTTGCT GGACACGATG GGTCCCTTGG ACTTGCTGAT GCTTCTTGAC 
               
               
                   
               
               
                   
                  K  H  L  L   S  R  I   N  H  F   E  K  I  Q   I  I  P • 
               
               
                 2151 
                 AAACACCTGC TGAGCAGAAT CAACCACTTT GAGAAAATCC AGATCATCCC 
               
               
                   
                 TTTGTGGACG ACTCGTCTTA GTTGGTGAAA CTCTTTTAGG TCTAGTAGGG 
               
               
                   
                                              11626JY 
               
               
                   
               
               
                   
                 . K  S  S   W  S  D  H   E  A  S   S  G  V   S  S  A  C • 
               
               
                 2201 
                 CAAAAGCAGC TGGTCCGATC ACGAAGCCAG CAGCGGAGTG AGCAGCGCCT 
               
               
                   
                 GTTTTCGTCG ACCAGGCTAG TGCTTCGGTC GTCGCCTCAC TCGTCGCGGA 
               
               
                   
                                              11527JY 
               
               
                   
               
               
                   
                 .. P  Y  Q   G  K  S   S  F  F  R   N  V  V   W  L  I 
               
               
                 2251 
                 GCCCATACCA GGGAAAGTCC AGCTTTTTTA GAAACGTGGT GTGGCTGATC 
               
               
                   
                 CGGGTATGGT CCCTTTCAGG TCGAAAAAAT CTTTGCACCA CACCGACTAG 
               
               
                   
               
               
                   
                  K  K  N  S   A  Y  P   T  I  K   R  S  Y  N   N  T  N • 
               
               
                 2301 
                 AAAAAGAACA GCGCCTACCC AACAATCAAG AGAAGCTACA ACAACACCAA 
               
               
                   
                 TTTTTCTTGT CGCGGATGGG TTGTTAGTTC TCTTCGATGT TGTTGTGGTT 
               
               
                   
               
               
                   
                 . Q  E  D   L  L  V  L   W  G  I   H  H  P   N  D  A  A • 
               
               
                 2351 
                 CCAGGAAGAT CTGCTGGTGC TGTGGGGGAT CCACCACCCT AACGATGCCG 
               
               
                   
                 GGTCCTTCTA GACGACCACG ACACCCCCTA GGTGGTGGGA TTGCTACGGC 
               
               
                   
               
               
                   
                 .. E  Q  T   R  L  Y   Q  N  P  T   T  Y  I   S  V  G 
               
               
                 2401 
                 CCGAGCAGAC AAGGCTGTAC CAGAACCCAA CCACCTACAT CTCCGTGGGG 
               
               
                   
                 GGCTCGTCTG TTCCGACATG GTCTTGGGTT GGTGGATGTA GAGGCACCCC 
               
               
                   
               
               
                   
                  T  S  T  L   N  Q  R   L  V  P   K  I  A  I   R  S  K • 
               
               
                 2451 
                 ACAAGCACAC TGAACCAGAG ACTGGTGCCA AAAATCGCCA TCAGATCCAA 
               
               
                   
                 TGTTCGTGTG ACTTGGTCTC TGACCACGGT TTTTAGCGGT AGTCTAGGTT 
               
               
                   
               
               
                   
                 . V  N  G   Q  S  G  R   M  E  F   F  W  T   I  L  K  P • 
               
               
                 2501 
                 AGTGAACGGG CAGAGCGGAA GAATGGAGTT CTTCTGGACA ATCCTGAAAC 
               
               
                   
                 TCACTTGCCC GTCTCGCCTT CTTACCTCAA GAAGACCTGT TAGGACTTTG 
               
               
                   
               
               
                   
                 .. N  D  A   I  N  F   E  S  N  G   N  F  I   A  P  E 
               
               
                 2551 
                 CCAACGATGC CATCAACTTC GAGAGCAACG GAAACTTCAT CGCCCCAGAA 
               
               
                   
                 GGTTGCTACG GTAGTTGAAG CTCTCGTTGC CTTTGAAGTA GCGGGGTCTT 
               
               
                   
               
               
                   
                  Y  A  Y  K   I  V  K   K  G  D   S  A  I  M   K  S  E • 
               
               
                 2601 
                 TACGCCTACA AAATCGTGAA GAAAGGGGAC AGCGCCATCA TGAAAAGCGA 
               
               
                   
                 ATGCGGATGT TTTAGCACTT CTTTCCCCTG TCGCGGTAGT ACTTTTCGCT 
               
               
                   
                                              11528JY 
               
               
                   
               
               
                   
                 . L  E  Y   G  N  C  N   T  K  C   Q  T  P   M  G  A  I • 
               
               
                 2651 
                 ACTGGAATAC GGCAACTGCA ACACCAAGTG CCAGACCCCA ATGGGGGCCA 
               
               
                   
                 TGACCTTATG CCGTTGACGT TGTGGTTCAC GGTCTGGGGT TACCCCCGGT 
               
               
                   
                                              11529JY 
               
               
                   
               
               
                   
                 .. N  S  S   M  P  F   H  N  I  H   P  L  T   I  G  E 
               
               
                 2701 
                 TCAACAGCAG CATGCCATTC CACAACATCC ACCCTCTGAC CATCGGGGAA 
               
               
                   
                 AGTTGTCGTC GTACGGTAAG GTGTTGTAGG TGGGAGACTG GTAGCCCCTT 
               
               
                   
               
               
                   
                 C  P  K  Y   V  K  S   N  R  L   V  L  A  T   G  L  R • 
               
               
                 2751 
                 TGCCCCAAAT ACGTGAAAAG CAACAGACTG GTGCTGGCCA CCGGGCTGAG 
               
               
                   
                 ACGGGGTTTA TGCACTTTTC GTTGTCTGAC CACGACCGGT GGCCCGACTC 
               
               
                   
               
               
                   
                 . N  S  P   Q  R  E  T   R  G  L   F  G  A   I  A  G  F • 
               
               
                 2801 
                 AAACAGCCCT CAGAGAGAGA CCAGAGGACT GTTTGGAGCC ATCGCCGGCT 
               
               
                   
                 TTTGTCGGGA GTCTCTCTCT GGTCTCCTGA CAAACCTCGG TAGCGGCCGA 
               
               
                   
               
               
                   
                 .. I  E  G   G  W  Q   G  M  V  D   G  W  Y   G  Y  H 
               
               
                 2851 
                 TTATCGAGGG AGGATGGCAG GGAATGGTGG ATGGCTGGTA CGGATACCAC 
               
               
                   
                 AATAGCTCCC TCCTACCGTC CCTTACCACC TACCGACCAT GCCTATGGTG 
               
               
                   
               
               
                   
                  H  S  N  E   Q  G  S   G  Y  A   A  D  K  E   S  T  Q • 
               
               
                 2901 
                 CACAGCAACG AGCAGGGGAG CGGATACGCC GCCGACAAAG AATCCACCCA 
               
               
                   
                 GTGTCGTTGC TCGTCCCCTC GCCTATGCGG CGGCTGTTTC TTAGGTGGGT 
               
               
                   
               
               
                   
                 . K  A  I   D  G  V  T   N  K  V   N  S  I   I  D  K  M • 
               
               
                 2951 
                 GAAGGCCATC GACGGCGTGA CCAACAAAGT GAACAGCATC ATCGACAAAA 
               
               
                   
                 CTTCCGGTAG CTGCCGCACT GGTTGTTTCA CTTGTCGTAG TAGCTGTTTT 
               
               
                   
               
               
                   
                 .. N  T  Q   F  E  A   V  G  R  E   F  N  N   L  E  R 
               
               
                 3001 
                 TGAACACCCA GTTTGAGGCC GTGGGAAGGG AGTTTAACAA CCTGGAAAGG 
               
               
                   
                 ACTTGTGGGT CAAACTCCGG CACCCTTCCC TCAAATTGTT GGACCTTTCC 
               
               
                   
               
               
                   
                  R  I  E  N   L  N  K   K  M  E   D  G  F  L   D  V  W • 
               
               
                 3051 
                 AGAATCGAGA ACCTGAACAA GAAGATGGAG GACGGATTCC TGGATGTGTG 
               
               
                   
                 TCTTAGCTCT TGGACTTGTT CTTCTACCTC CTGCCTAAGG ACCTACACAC 
               
               
                   
                                11530JY 
               
               
                   
               
               
                   
                 . T  Y  N   A  E  L  L   V  L  M   E  N  E   R  T  L  D • 
               
               
                 3101 
                 GACCTACAAC GCCGAACTGC TGGTGCTGAT GGAAAACGAG AGAACCCTGG 
               
               
                   
                 CTGGATGTTG CGGCTTGACG ACCACGACTA CCTTTTGCTC TCTTGGGACC 
               
               
                   
                                11531JY 
               
               
                   
               
               
                   
                 .. F  H  D   S  N  V   K  N  L  Y   D  K  V   R  L  Q 
               
               
                 3151 
                 ACTTTCACGA CAGCAACGTG AAGAACCTGT ACGACAAAGT GAGGCTGCAG 
               
               
                   
                 TGAAAGTGCT GTCGTTGCAC TTCTTGGACA TGCTGTTTCA CTCCGACGTC 
               
               
                   
               
               
                   
                  L  R  D  N   A  K  E   L  G  N   G  C  F  E   F  Y  H • 
               
               
                 3201 
                 CTGAGGGATA ACGCCAAGGA GCTGGGCAAC GGCTGCTTCG AGTTCTACCA 
               
               
                   
                 GACTCCCTAT TGCGGTTCCT CGACCCGTTG CCGACGAAGC TCAAGATGGT 
               
               
                   
               
               
                   
                 . K  C  D   N  E  C  M   E  S  I   R  N  G   T  Y  N  Y • 
               
               
                 3251 
                 CAAATGCGAT AACGAATGCA TGGAAAGCAT CAGAAACGGA ACCTACAACT 
               
               
                   
                 GTTTACGCTA TTGCTTACGT ACCTTTCGTA GTCTTTGCCT TGGATGTTGA 
               
               
                   
               
               
                   
                 .. P  Q  Y   S  E  E   A  R  L  K   R  E  E   I  S  G 
               
               
                 3301 
                 ACCCCCAGTA CAGCGAAGAA GCCAGACTGA AAAGAGAAGA AATCTCCGGA 
               
               
                   
                 TGGGGGTCAT GTCGCTTCTT CGGTCTGACT TTTCTCTTCT TTAGAGGCCT 
               
               
                   
               
               
                   
                  V  K  L  E   S  I  G   T  Y  Q   I  L  S  I   Y  S  T • 
               
               
                 3351 
                 GTGAAACTGG AATCCATCGG AACCTACCAG ATCCTGAGCA TCTACAGCAC 
               
               
                   
                 CACTTTGACC TTAGGTAGCC TTGGATGGTC TAGGACTCGT AGATGTCGTG 
               
               
                   
               
               
                   
                 . V  A  S   S  L  A  L   A  I  M   M  A  G   L  S  L  W • 
               
               
                 3401 
                 AGTGGCCTCC TCCCTGGCCC TGGCCATCAT GATGGCCGGA CTGAGCCTGT 
               
               
                   
                 TCACCGGAGG AGGGACCGGG ACCGGTAGTA CTACCGGCCT GACTCGGACA 
               
               
                   
                                11532JY 
               
               
                   
               
               
                   
                 .. M  C  S   N  G  S   L  Q  C  R   I  C  I   * 
               
               
                 3451 
                 GGATGTGCTC CAACGGAAGC CTGCAGTGCA GAATCTGCAT CTGACTCGAG 
               
               
                   
                 CCTACACGAG GTTGCCTTCG GACGTCACGT CTTAGACGTA GACTGAGCTC 
               
               
                   
                                11533JY           C5L 
               
               
                   
               
               
                 3501 
                 TTTTTATTGA CTAGTTAATC ACGGCCGCTT ATAAAGATCT AAAATGCATA 
               
               
                   
                 AAAAATAACT GATCAATTAG TGCCGGCGAA TATTTCTAGA TTTTACGTAT 
               
               
                   
                                                    7928.DC 
               
               
                   
               
               
                 3551 
                 ATTTCTAAAT AATGAAAAAA AGTACATCAT GAGCAACGCG TTAGTATATT 
               
               
                   
                 TAAAGATTTA TTACTTTTTT TCATGTAGTA CTCGTTGCGC AATCATATAA 
               
               
                   
               
               
                 3601 
                 TTACAATGGA GATTAACGCT CTATACCGTT CTATGTTTAT TGATTCAGAT 
               
               
                   
                 AATGTTACCT CTAATTGCGA GATATGGCAA GATACAAATA ACTAAGTCTA 
               
               
                   
                                     7793.SL 
               
               
                   
               
               
                 3651 
                 GATGTTTTAG AAAAGAAAGT TATTGAATAT GAAAACTTTA ATGAAGATGA 
               
               
                   
                 CTACAAAATC TTTTCTTTCA ATAACTTATA CTTTTGAAAT TACTTCTACT 
               
               
                   
               
               
                 3701 
                 AGATGACGAC GATGATTATT GTTGTAAATC TGTTTTAGAT GAAGAAGATG 
               
               
                   
                 TCTACTGCTG CTACTAATAA CAACATTTAG ACAAAATCTA CTTCTTCTAC 
               
               
                   
               
               
                 3751 
                 ACGCGCTAAA GTATACTATG GTTACAAAGT ATAAGTCTAT ACTACTAATG 
               
               
                   
                 TGCGCGATTT CATATGATAC CAATGTTTCA TATTCAGATA TGATGATTAC 
               
               
                   
               
               
                 3801 
                 GCGACTTGTG CAAGAAGGTA TAGTATAGTG AAAATGTTGT TAGATTATGA 
               
               
                   
                 CGCTGAACAC GTTCTTCCAT ATCATATCAC TTTTACAACA ATCTAATACT 
               
               
                   
               
               
                 3851 
                 TTATGAAAAA CCAAATAAAT CAGATCCATA TCTAAAGGTA TCTCCTTTGC 
               
               
                   
                 AATACTTTTT GGTTTATTTA GTCTAGGTAT AGATTTCCAT AGAGGAAACG 
               
               
                   
                                                7929.DC 
               
               
                   
               
               
                 3901 
                 ACATAATTTC ATCTATTCCT AGTTTAGAAT ACCTGCAGCC AAGCTTGGCA 
               
               
                   
                 TGTATTAAAG TAGATAAGGA TCAAATCTTA TGGACGTCGG TTCGAACCGT 
               
               
                   
               
               
                 3951 
                 CTGGCCGTCG TTTTAC 
               
               
                   
                 GACCGGCAGC AAAATG 
               
               
                   
                       M13F 
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 19 
                   
               
               
                 LENGTH: 6551 
                   
               
               
                   
               
               
                 OTHER INFORMATION: Theoretical sequence of entire vector 
               
               
                   
               
               
                 SEQUENCE: 
               
               
                 gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact 
               
               
                   
               
               
                 ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatg 
               
               
                   
               
               
                 cttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacga 
               
               
                   
               
               
                 attgcggccgcaattctgaatgttaaatgttatactttggatgaagctataaatatgcattggaaaaataatccatt 
               
               
                   
               
               
                 taaagaaaggattcaaatactacaaaacctaagcgataatatgttaactaagcttattcttaacgacgctttaaata 
               
               
                   
               
               
                 tacacaaataaacataatttttgtataacctaacaaataactaaaacataaaaataataaaaggaaatgtaatatcg 
               
               
                   
               
               
                 taattattttactcaggaatggggttaaatatttatatcacgtgtatatctatactgttatcgtatactctttacaa 
               
               
                   
               
               
                 ttactattacgaatatgcaagagataataagattacgtatttaagagaatcttgtcatgataattgggtacgacata 
               
               
                   
               
               
                 gtgataaatgctatttcgcatcgttacataaagtcagttggaaagatggatttgacagatgtaacttaataggtgca 
               
               
                   
               
               
                 aaaatgttaaataacagcattctatcggaagataggataccagttatattatacaaaaatcactggttggataaaac 
               
               
                   
               
               
                 agattctgcaatattcgtaaaagatgaagattactgcgaatttgtaaactatgacaataaaaagccatttatctcaa 
               
               
                   
               
               
                 cgacatcgtgtaattcttccatgttttatgtatgtgtttcagatattatgagattactataaactttttgtatactt 
               
               
                   
               
               
                 atattccgtaaactatattaatcatgaagaaaatgaaaaagtatagaagctgttcacgagcggttgttgaaaacaac 
               
               
                   
               
               
                 aaaattatacattcaagatggcttacatatacgtctgtgaggctatcatggataatgacaatgcatctctaaatagg 
               
               
                   
               
               
                 tttttggacaatggattcgaccctaacacggaatatggtactctacaatctcctcttgaaatggctgtaatgttcaa 
               
               
                   
               
               
                 gaataccgaggctataaaaatcttgatgaggtatggagctaaacctgtagttactgaatgcacaacttcttgtctgc 
               
               
                   
               
               
                 atgatgcggtgttgagagacgactacaaaatagtgaaagatctgttgaagaataactatgtaaacaatgttctttac 
               
               
                   
               
               
                 agcggaggctttactcctttgtgtttggcagcttaccttaacaaagttaatttggttaaacttctattggctcattc 
               
               
                   
               
               
                 ggcggatgtagatatttcaaacacggatcggttaactcctctacatatagccgtatcaaataaaaatttaacaatgg 
               
               
                   
               
               
                 ttaaacttctattgaacaaaggtgctgatactgacttgctggataacatgggacgtactcctttaatgatcgctgta 
               
               
                   
               
               
                 caatctggaaatattgaaatatgtagcacactacttaaaaaaaataaaatgtccagaactgggaaaaattgatcttg 
               
               
                   
               
               
                 ccagctgtaattcatggtagaaaagaagtgctcaggctacttttcaacaaaggagcagatgtaaactacatctttga 
               
               
                   
               
               
                 aagaaatggaaaatcatatactgttttggaattgattaaagaaagttactctgagacacaaaagaggtagctgaagt 
               
               
                   
               
               
                 ggtactctcaaaggtacgtgactaattagctataaaaaggatccgggttaattaattagtcatcaggcagggcgaga 
               
               
                   
               
               
                 acgagactatctgctcgttaattaattagagcttctttattctatacttaaaaagtgaaaataaatacaaaggttct 
               
               
                   
               
               
                 tgagggttgtgttaaattgaaagcgagaaataatcataaattatttcattatcgcgatatccgttaagtttgtatcg 
               
               
                   
               
               
                 taatggagaaaatcgtgctgctgctggccatcgtgagcctggtgaaaagcgatcagatctgcatcggctaccacgcc 
               
               
                   
               
               
                 aacaacagcacagagcaagtggacacaatcatggaaaagaacgtgaccgtgacacacgcccaggacatcctggaaaa 
               
               
                   
               
               
                 gacacacaacgggaagctgtgcgatctggatggagtgaagcctctgatcctgagagattgcagcgtggccggatggc 
               
               
                   
               
               
                 tgctggggaacccaatgtgcgacgaattcatcaacgtgcccgaatggagctacatcgtggagaaggccaacccagcc 
               
               
                   
               
               
                 aacgacctgtgctacccagggaacctgaacgactacgaagaactgaaacacctgctgagcagaatcaaccactttga 
               
               
                   
               
               
                 gaaaatccagatcatccccaaaagcagctggtccgatcacgaagccagcagcggagtgagcagcgcctgcccatacc 
               
               
                   
               
               
                 agggaaagtccagcttttttagaaacgtggtgtggctgatcaaaaagaacagcgcctacccaacaatcaagagaagc 
               
               
                   
               
               
                 tacaacaacaccaaccaggaagatctgctggtgctgtgggggatccaccaccctaacgatgccgccgagcagacaag 
               
               
                   
               
               
                 gctgtaccagaacccaaccacctacatctccgtggggacaagcacactgaaccagagactggtgccaaaaatcgcca 
               
               
                   
               
               
                 tcagatccaaagtgaacgggcagagcggaagaatggagttcttctggacaatcctgaaacccaacgatgccatcaac 
               
               
                   
               
               
                 ttcgagagcaacggaaacttcatcgccccagaatacgcctacaaaatcgtgaagaaaggggacagcgccatcatgaa 
               
               
                   
               
               
                 aagcgaactggaatacggcaactgcaacaccaagtgccagaccccaatgggggccatcaacagcagcatgccattcc 
               
               
                   
               
               
                 acaacatccaccctctgaccatcggggaatgccccaaatacgtgaaaagcaacagactggtgctggccaccgggctg 
               
               
                   
               
               
                 agaaacagccctcagagagagaccagaggactgtttggagccatcgccggctttatcgagggaggatggcagggaat 
               
               
                   
               
               
                 ggtggatggctggtacggataccaccacagcaacgagcaggggagcggatacgccgccgacaaagaatccacccaga 
               
               
                   
               
               
                 aggccatcgacggcgtgaccaacaaagtgaacagcatcatcgacaaaatgaacacccagtttgaggccgtgggaagg 
               
               
                   
               
               
                 gagtttaacaacctggaaaggagaatcgagaacctgaacaagaagatggaggacggattcctggatgtgtggaccta 
               
               
                   
               
               
                 caacgccgaactgctggtgctgatggaaaacgagagaaccctggactttcacgacagcaacgtgaagaacctgtacg 
               
               
                   
               
               
                 acaaagtgaggctgcagctgagggataacgccaaggagctgggcaacggctgcttcgagttctaccacaaatgcgat 
               
               
                   
               
               
                 aacgaatgcatggaaagcatcagaaacggaacctacaactacccccagtacagcgaagaagccagactgaaaagaga 
               
               
                   
               
               
                 agaaatctccggagtgaaactggaatccatcggaacctaccagatcctgagcatctacagcacagtggcctcctccc 
               
               
                   
               
               
                 tggccctggccatcatgatggccggactgagcctgtggatgtgctccaacggaagcctgcagtgcagaatctgcatc 
               
               
                   
               
               
                 tgactcgagtttttattgactagttaatcacggccgcttataaagatctaaaatgcataatttctaaataatgaaaa 
               
               
                   
               
               
                 aaagtacatcatgagcaacgcgttagtatattttacaatggagattaacgctctataccgttctatgtttattgatt 
               
               
                   
               
               
                 cagatgatgttttagaaaagaaagttattgaatatgaaaactttaatgaagatgaagatgacgacgatgattattgt 
               
               
                   
               
               
                 tgtaaatctgttttagatgaagaagatgacgcgctaaagtatactatggttacaaagtataagtctatactactaat 
               
               
                   
               
               
                 ggcgacttgtgcaagaaggtatagtatagtgaaaatgttgttagattatgattatgaaaaaccaaataaatcagatc 
               
               
                   
               
               
                 catatctaaaggtatctcctttgcacataatttcatctattcctagtttagaatacctgcagccaagcttggcactg 
               
               
                   
               
               
                 gccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatcccccttt 
               
               
                   
               
               
                 cgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggc 
               
               
                   
               
               
                 gcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgc 
               
               
                   
               
               
                 tctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctccc 
               
               
                   
               
               
                 ggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaac 
               
               
                   
               
               
                 gcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtca 
               
               
                   
               
               
                 ggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgct 
               
               
                   
               
               
                 catgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcg 
               
               
                   
               
               
                 cccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgct 
               
               
                   
               
               
                 gaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccc 
               
               
                   
               
               
                 cgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggc 
               
               
                   
               
               
                 aagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatctt 
               
               
                   
               
               
                 acggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttct 
               
               
                   
               
               
                 gacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgtt 
               
               
                   
               
               
                 gggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttg 
               
               
                   
               
               
                 cgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagt 
               
               
                   
               
               
                 tgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggt 
               
               
                   
               
               
                 ctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcag 
               
               
                   
               
               
                 gcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagacca 
               
               
                   
               
               
                 agtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttg 
               
               
                   
               
               
                 ataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaagga 
               
               
                   
               
               
                 tcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttg 
               
               
                   
               
               
                 tttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtcc 
               
               
                   
               
               
                 ttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctg 
               
               
                   
               
               
                 ttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggc 
               
               
                   
               
               
                 gcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacc 
               
               
                   
               
               
                 tacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtc 
               
               
                   
               
               
                 ggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacct 
               
               
                   
               
               
                 ctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt 
               
               
                   
               
               
                 tacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgt 
               
               
                   
               
               
                 attaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagc 
               
               
                   
               
               
                 ggaaga 
               
               
                   
               
               
                 SEQ ID NO 20 
                   
               
               
                 LENGTH: 25 
                   
               
               
                   
               
               
                 OTHER INFORMATION: Primer for PCR amplification of the vCP2241 C5 arms plus 
               
               
                 insert, referred to as 7931.DC 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 GAATCTGTTAGTTAGTTACTTGGAT 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 21 
                   
               
               
                 LENGTH: 25 
                   
               
               
                   
               
               
                 OTHER INFORMATION: Primer for PCR amplification of the vCP2241 C5 arms plus 
               
               
                 insert, referred to as 7932.DC 
               
               
                   
               
               
                 SEQUENCE: 
               
            
           
           
               
               
               
            
               
                 1 
                 TGATTATAGCTATTATCACAGACTC 
                   
               
               
                   
               
            
           
           
               
               
            
               
                 SEQ ID NO 22 
                   
               
               
                 LENGTH: ORGANISM: Artificial Sequence 
                   
               
               
                   
               
               
                 OTHER INFORMATION: C5 right arm (1-1536), H6 promoter (1634-1757), AIV 
               
               
                 synethetic H5 HA (1758-3452), and C5 left arm (3485-3890) determined through 
               
               
                 sequence analysis SEQUENCE: 
               
            
           
           
               
               
               
            
               
                   
                 TGAATGTTAAATGTTATACTTTGGATGAAGCTATAAATATGCATTGGAAA 
                   
               
               
                   
               
               
                   
                 AATAATCCATTTAAAGAAAGGATTCAAATACTACAAAACCTAAGCGATAA 
               
               
                   
               
               
                   
                 TATGTTAACTAAGCTTATTCTTAACGACGCTTTAAATATACACAAATAAA 
               
               
                   
               
               
                   
                 CATAATTTTTGTATAACCTAACAAATAACTAAAACATAAAAATAATAAAA 
               
               
                   
               
               
                   
                 GGAAATGTAATATCGTAATTATTTTACTCAGGAATGGGGTTAAATATTTA 
               
               
                   
               
               
                   
                 TATCACGTGTATATCTATACTGTTATCGTATACTCTTTACAATTACTATT 
               
               
                   
               
               
                   
                 ACGAATATGCAAGAGATAATAAGATTACGTATTTAAGAGAATCTTGTCAT 
               
               
                   
               
               
                   
                 GATAATTGGGTACGACATAGTGATAAATGCTATTTCGCATCGTTACATAA 
               
               
                   
               
               
                   
                 AGTCAGTTGGAAAGATGGATTTGACAGATGTAACTTAATAGGTGCAAAAA 
               
               
                   
               
               
                   
                 TGTTAAATAACAGCATTCTATCGGAAGATAGGATACCAGTTATATTATAC 
               
               
                   
               
               
                   
                 AAAAATCACTGGTTGGATAAAACAGATTCTGCAATATTCGTAAAAGATGA 
               
               
                   
               
               
                   
                 AGATTACTGCGAATTTGTAAACTATGACAATAAAAAGCCATTTATCTCAA 
               
               
                   
               
               
                   
                 CGACATCGTGTAATTCTTCCATGTTTTATGTATGTGTTTCAGATATTATG 
               
               
                   
               
               
                   
                 AGATTACTATAAACTTTTTGTATACTTATATTCCGTAAACTATATTAATC 
               
               
                   
               
               
                   
                 ATGAAGAAAATGAAAAAGTATAGAAGCTGTTCACGAGCGGTTGTTGAAAA 
               
               
                   
               
               
                   
                 CAACAAAATTATACATTCAAGATGGCTTACATATACGTCTGTGAGGCTAT 
               
               
                   
               
               
                   
                 CATGGATAATGACAATGCATCTCTAAATAGGTTTTTGGACAATGGATTCG 
               
               
                   
               
               
                   
                 ACCCTAACACGGAATATGGTACTCTACAATCTCCTCTTGAAATGGCTGTA 
               
               
                   
               
               
                   
                 ATGTTCAAGAATACCGAGGCTATAAAAATCTTGATGAGGTATGGAGCTAA 
               
               
                   
               
               
                   
                 ACCTGTAGTTACTGAATGCACAACTTCTTGTCTGCATGATGCGGTGTTGA 
               
               
                   
               
               
                   
                 GAGACGACTACAAAATAGTGAAAGATCTGTTGAAGAATAACTATGTAAAC 
               
               
                   
               
               
                   
                 AATGTTCTTTACAGCGGAGGCTTTACTCCTTTGTGTTTGGCAGCTTACCT 
               
               
                   
               
               
                   
                 TAACAAAGTTAATTTGGTTAAACTTCTATTGGCTCATTCGGCGGATGTAG 
               
               
                   
               
               
                   
                 ATATTTCAAACACGGATCGGTTAACTCCTCTACATATAGCCGTATCAAAT 
               
               
                   
               
               
                   
                 AAAAATTTAACAATGGTTAAACTTCTATTGAACAAAGGTGCTGATACTGA 
               
               
                   
               
               
                   
                 CTTGCTGGATAACATGGGACGTACTCCTTTAATGATCGCTGTACAATCTG 
               
               
                   
               
               
                   
                 GAAATATTGAAATATGTAGCACACTACTTAAAAAAAATAAAATGTCCAGA 
               
               
                   
               
               
                   
                 ACTGGGAAAAATTGATCTTGCCAGCTGTAATTCATGGTAGAAAAGAAGTG 
               
               
                   
               
               
                   
                 CTCAGGCTACTTTTCAACAAAGGAGCAGATGTAAACTACATCTTTGAAAG 
               
               
                   
               
               
                   
                 AAATGGAAAATCATATACTGTTTTGGAATTGATTAAAGAAAGTTACTCTG 
               
               
                   
               
               
                   
                 AGACACAAAAGAGGTAGCTGAAGTGGTACTCTCAAAGGTACGTGACTAAT 
               
               
                   
               
               
                   
                 TAGCTATAAAAAGGATCCGGGTTAATTAATTAGTCATCAGGCAGGGCGAG 
               
               
                   
               
               
                   
                 AACGAGACTATCTGCTCGTTAATTAATTAGAGCTTCTTTATTCTATACTT 
               
               
                   
               
               
                   
                 AAAAAGTGAAAATAAATACAAAGGTTCTTGAGGGTTGTGTTAAATTGAAA 
               
               
                   
               
               
                   
                 GCGAGAAATAATCATAAATTATTTCATTATCGCGATATCCGTTAAGTTTG 
               
               
                   
               
               
                   
                 TATCGTAATGGAGAAAATCGTGCTGCTGCTGGCCATCGTGAGCCTGGTGA 
               
               
                   
               
               
                   
                 AAAGCGATCAGATCTGCATCGGCTACCACGCCAACAACAGCACAGAGCAA 
               
               
                   
               
               
                   
                 GTGGACACAATCATGGAAAAGAACGTGACCGTGACACACGCCCAGGACAT 
               
               
                   
               
               
                   
                 CCTGGAAAAGACACACAACGGGAAGCTGTGCGATCTGGATGGAGTGAAGC 
               
               
                   
               
               
                   
                 CTCTGATCCTGAGAGATTGCAGCGTGGCCGGATGGCTGCTGGGGAACCCA 
               
               
                   
               
               
                   
                 ATGTGCGACGAATTCATCAACGTGCCCGAATGGAGCTACATCGTGGAGAA 
               
               
                   
               
               
                   
                 GGCCAACCCAGCCAACGACCTGTGCTACCCAGGGAACCTGAACGACTACG 
               
               
                   
               
               
                   
                 AAGAACTGAAACACCTGCTGAGCAGAATCAACCACTTTGAGAAAATCCAG 
               
               
                   
               
               
                   
                 ATCATCCCCAAAAGCAGCTGGTCCGATCACGAAGCCAGCAGCGGAGTGAG 
               
               
                   
               
               
                   
                 CAGCGCCTGCCCATACCAGGGAAAGTCCAGCTTTTTTAGAAACGTGGTGT 
               
               
                   
               
               
                   
                 GGCTGATCAAAAAGAACAGCGCCTACCCAACAATCAAGAGAAGCTACAAC 
               
               
                   
               
               
                   
                 AACACCAACCAGGAAGATCTGCTGGTGCTGTGGGGGATCCACCACCCTAA 
               
               
                   
               
               
                   
                 CGATGCCGCCGAGCAGACAAGGCTGTACCAGAACCCAACCACCTACATCT 
               
               
                   
               
               
                   
                 CCGTGGGGACAAGCACACTGAACCAGAGACTGGTGCCAAAAATCGCCATC 
               
               
                   
               
               
                   
                 AGATCCAAAGTGAACGGGCAGAGCGGAAGAATGGAGTTCTTCTGGACAAT 
               
               
                   
               
               
                   
                 CCTGAAACCCAACGATGCCATCAACTTCGAGAGCAACGGAAACTTCATCG 
               
               
                   
               
               
                   
                 CCCCAGAATACGCCTACAAAATCGTGAAGAAAGGGGACAGCGCCATCATG 
               
               
                   
               
               
                   
                 AAAAGCGAACTGGAATACGGCAACTGCAACACCAAGTGCCAGACCCCAAT 
               
               
                   
               
               
                   
                 GGGGGCCATCAACAGCAGCATGCCATTCCACAACATCCACCCTCTGACCA 
               
               
                   
               
               
                   
                 TCGGGGAATGCCCCAAATACGTGAAAAGCAACAGACTGGTGCTGGCCACC 
               
               
                   
               
               
                   
                 GGGCTGAGAAACAGCCCTCAGAGAGAGACCAGAGGACTGTTTGGAGCCAT 
               
               
                   
               
               
                   
                 CGCCGGCTTTATCGAGGGAGGATGGCAGGGAATGGTGGATGGCTGGTACG 
               
               
                   
               
               
                   
                 GATACCACCACAGCAACGAGCAGGGGAGCGGATACGCCGCCGACAAAGAA 
               
               
                   
               
               
                   
                 TCCACCCAGAAGGCCATCGACGGCGTGACCAACAAAGTGAACAGCATCAT 
               
               
                   
               
               
                   
                 CGACAAAATGAACACCCAGTTTGAGGCCGTGGGAAGGGAGTTTAACAACC 
               
               
                   
               
               
                   
                 TGGAAAGGAGAATCGAGAACCTGAACAAGAAGATGGAGGACGGATTCCTG 
               
               
                   
               
               
                   
                 GATGTGTGGACCTACAACGCCGAACTGCTGGTGCTGATGGAAAACGAGAG 
               
               
                   
               
               
                   
                 AACCCTGGACTTTCACGACAGCAACGTGAAGAACCTGTACGACAAAGTGA 
               
               
                   
               
               
                   
                 GGCTGCAGCTGAGGGATAACGCCAAGGAGCTGGGCAACGGCTGCTTCGAG 
               
               
                   
               
               
                   
                 TTCTACCACAAATGCGATAACGAATGCATGGAAAGCATCAGAAACGGAAC 
               
               
                   
               
               
                   
                 CTACAACTACCCCCAGTACAGCGAAGAAGCCAGACTGAAAAGAGAAGAAA 
               
               
                   
               
               
                   
                 TCTCCGGAGTGAAACTGGAATCCATCGGAACCTACCAGATCCTGAGCATC 
               
               
                   
               
               
                   
                 TACAGCACAGTGGCCTCCTCCCTGGCCCTGGCCATCATGATGGCCGGACT 
               
               
                   
               
               
                   
                 GAGCCTGTGGATGTGCTCCAACGGAAGCCTGCAGTGCAGAATCTGCATCT 
               
               
                   
               
               
                   
                 GACTCGAGTTTTTATTGACTAGTTAATCACGGCCGCTTATAAAGATCTAA 
               
               
                   
               
               
                   
                 AATGCATAATTTCTAAATAATGAAAAAAAGTACATCATGAGCAACGCGTT 
               
               
                   
               
               
                   
                 AGTATATTTTACAATGGAGATTAACGCTCTATACCGTTCTATGTTTATTG 
               
               
                   
               
               
                   
                 ATTCAGATGATGTTTTAGAAAAGAAAGTTATTGAATATGAAAACTTTAAT 
               
               
                   
               
               
                   
                 GAAGATGAAGATGACGACGATGATTATTGTTGTAAATCTGTTTTAGATGA 
               
               
                   
               
               
                   
                 AGAAGATGACGCGCTAAAGTATACTATGGTTACAAAGTATAAGTCTATAC 
               
               
                   
               
               
                   
                 TACTAATGGCGACTTGTGCAAGAAGGTATAGTATAGTGAAAATGTTGTTA 
               
               
                   
               
               
                   
                 GATTATGATTATGAAAAACCAAATAAATCAGATCCATATCTAAAGGTATC 
               
               
                   
               
               
                   
                 TCCTTTGCACATAATTTCATCTATTCCTAGTTTAGAATAC