Patent Publication Number: US-2022235146-A1

Title: ANTI-IgE ANTIBODIES

Description:
REFERENCE TO AN ELECTRONIC SEQUENCE LISTING 
     The contents of the electronic sequence listing (0089-0022US1_SL.txt; Size: 123,000 bytes; and Date of Creation Nov. 13, 2018) is herein incorporated by reference in its entirety. 
     FIELD OF THE INVENTION 
     This invention is in the area of improved anti-IgE antibodies and antigen binding agents, and compositions thereof, which target IgE, for instance for use in treating disorders caused by IgE (such as allergic responses, or certain autoimmune responses), and in particular disorders caused by the interaction of IgE with the FcεRI receptor. In particular, this invention relates to improved anti-IgE antibodies and antigen binding agents related to novel mutants of omalizumab (Xolair®). The improved anti-IgE antibodies and antigen binding agents of the invention may have improved affinity for IgE and/or an improved interaction with the Cε2 domain of IgE and/or an improved modified epitope on IgE (for instance further involving the Cε2 domain of IgE) and/or the ability to disassociate IgE from the FcεRI receptor at pharmaceutically-relevant concentrations. In one aspect, improved or novel treatments for IgE mediated disorders are disclosed in which IgE is targeted (for instance free IgE and/or IgE complexed with the FcεRI receptor). 
     BACKGROUND OF THE INVENTION 
     IgE is a member of the immunoglobulin family that mediates allergic responses such as asthma, food allergies, type 1 hypersensitivity and the familiar sinus inflammation suffered on a widespread basis. IgE is secreted by, and expressed on the surface of, B-cells. IgE synthesized by B-cells is anchored in the B-cell membrane by a transmembrane domain linked to the mature IgE sequence by a short membrane binding region. IgE also is bound to B-cells (and monocytes, eosinophils and platelets) through its Fc region to a low affinity IgE receptor (FcεRII). Upon exposure of a mammal to an allergen, B-cells are clonally amplified which synthesize IgE that binds the allergen. This IgE in turn is released into the circulation by the B-cells where it is bound by B-cells (through FcεRII) and by mast cells and basophils through the so-called high affinity receptor (FcεRI) found on the surface of the mast cells ad basophils. Such mast cells and basophils are thereby sensitized for allergen. The next exposure to the allergen cross-links the FcεRI on these cells and thus activate their release of histamine and other factors which are responsible for clinical hypersensitivity and anaphylaxis. 
     Omalizumab (Xolair®) is a recombinant DNA-derived humanized IgG1κ monoclonal antibody that selectively binds to human immunoglobulin E (IgE) [the Cε3 domain]. The antibody has a molecular weight of approximately 149 kD. Xolair® is produced by a Chinese hamster ovary cell suspension culture in a nutrient medium containing the antibiotic gentamicin. Xolair® is a sterile, white, preservative-free, lyophilized powder contained in a single-use vial that is reconstituted with Sterile Water for Injection (SWFI), USP, (or, alternatively, as a liquid formulation in a sterile syringe) and administered as a subcutaneous (SC) injection [see EP602126 (and SPC/GB06/005 based thereon); WO93/04173; U.S. Pat. No. 6,267,958 (and the Xolair® PTE based on this patent); WO97/04807; WO97/04801; Presta et al. (1993) J. Immunol. 151:2623-2632]. 
     Omalizumab is presently indicated for the treatment of moderate to severe persistent asthma in patients with a positive skin test or in vitro reactivity to a perennial aeroallergen and symptoms that are inadequately controlled by inhaled corticosteroids (from Xolair® Prescribing Information). 
     Problems exist with omalizumab in that: 1) it targets free IgE but does not (or does not efficiently) target the pathogenic species of the IgE/FcεRI complex at pharmaceutically-relevant doses; 2) possibly due to the pathogenic species of the IgE/FcεRI complex not being targeted, it takes “at least 12-16 weeks for Xolair treatment to show effectiveness” (Xolair® 150 mg solution—Summary of Product Characteristics 2014)—or indeed to establish whether Xolair® will work for a particular patient or whether a different treatment is necessitated; 3) it should not be for patients with high levels of IgE (for instance because the pathogenic species of the IgE/FcεRI complex is not targeted and does not dissipate with time given the high levels of free IgE in the patient); 4) “Type I local or systemic reactions, including anaphylaxis and anaphylactic shock, may occur when taking omalizumab” (Xolair® 150 mg solution—Summary of Product Characteristics 2014); 5) its affinity for IgE is not particular good (approximately 2 nM). 
     It is an object of the present invention to identify novel antibodies to ameliorate one or more of these problems. 
     A further object is to identify antibodies against novel epitopes (with an increased IgE Cε2 interaction compared with omalizumab), and/or antibodies based on novel mutants of omalizumab with improved affinity and/or improved ability to disassociate the IgE/FcεRI complex. 
     A still further object of the invention to identify new compounds, methods, and compositions for the treatment of disorders associated with IgE, in particular disorders associated with the complex of IgE/FcεRI, for instance allergic disorders. 
     SUMMARY OF THE INVENTION 
     In one aspect of the invention an anti-IgE antibody, or antigen binding agent, is provided which contacts an epitope comprising residues T373, W374, S375, R376, A377, S378, G379, P381, Q417, C418, R419, T421, P426, R427, A428 of a Cε3 domain and residues D278 and T281 of a Cε2 domain of human IgE. In further embodiments the epitope may further comprise one or more of residues K380 and/or M430 of the Cε3 domain of human IgE and/or one or more of residues D276, V277, L279, S280, A282 and/or T298 of the Cε2 domain of human IgE. 
     The invention is based on the observations of the crystal structure of Example 1 which, for the first time, shows the interaction of an improved antibody (based on omalizumab) with IgE-Fc where significant interactions were observed with the IgE Cε2 domain in the region of mutation. This may result in improved functional characteristics of the anti-IgE antibody, or antigen binding agent, relative to omalizumab and/or omalizumab Fab. For instance, the anti-IgE antibody, or antigen binding agent, may be capable of disassociating human IgE from FcεRI at concentrations (or peak serum concentrations) of less than 7, 3, 1, 0.66, 0.5 or 0.3 μM (for instance as carried out by the method described in Example 2). For instance, the anti-IgE antibody, or antigen binding agent, may have an improved/stronger affinity (lower K D ) for human IgE (for instance using IgE-Fc) (for example as carried out by the method described in Example 6) relative to omalizumab and/or omalizumab Fab; and/or an improved ability to disassociate the IgE/FcεRI complex (for instance as determined by the method described in Example 2), relative to omalizumab and/or omalizumab Fab; and/or a capability of disassociating human IgE from FcεRI at concentrations (or peak serum concentrations) lower than for omalizumab and/or omalizumab Fab (for instance as determined by the method described in Example 2). By an improved K D  it is meant at least 5, 10, 20, 30, 40, or 50% lower than that of omalizumab and/or omalizumab Fab. The K D  of the anti-IgE antibody, or antigen binding agent, of the invention may be less than 2, 1.9, 1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, or 0.3 nM. By an improved ability or capability of disassociating human IgE from FcεRI it is meant at least 5, 10, 20, 30, 40, 50, or 100% improved over omalizumab and/or omalizumab Fab (for instance when measuring the % disassociation and/or apparent disassociation rate of the IgE/FcεRI complex as described in Examples 2 and 7), and/or the achievement of disassociation at a concentration where omalizumab and/or omalizumab Fab does not achieve disassociation. 
     For the avoidance of doubt, the anti-IgE antibody, or antigen binding agent, of the invention is not omalizumab or omalizumab Fab. 
     In one embodiment, the epitope is determined crystallographically (for Example as described in Example 1) by determining IgE residues within 4 or 5 Å of the anti-IgE antibody, or antigen binding agent, in a crystal structure of complexed IgE-Fc/anti-IgE antibody, or antigen binding agent. The IgE-Fc used may be as that of Seq. ID No. 108 (with the additional N265Q &amp; N371Q mutations). 
     In one embodiment, the anti-IgE antibody, or antigen binding agent, at a particular binding site, contacts the epitope wherein the Cε3 domain and the Cε2 domain parts of the epitope are on different chains of the human IgE. IgE has two chains in the Fc domain each with a Cε3 domain and a Cε2 domain. 
     In one embodiment, the anti-IgE antibody, or antigen binding agent, at a particular binding site, contacts the epitope wherein the Cε3 domain and the Cε2 domain parts of the epitope are on the same chain of the human IgE. 
     For the avoidance of doubt, two anti-IgE antibodies, or antigen binding agents, of the invention may bind to human IgE, but only one of these needs interact with the epitope of the invention comprising Cε3 and Cε2 domains (the other may only interact with the other Cε3 domain for instance). 
     In one embodiment (optionally further adopting the features of the first aspect of the invention) the anti-IgE antibody, or antigen binding agent, is specific for said epitope comprising residues T373, W374, S375, R376, A377, S378, G379, P381, Q417, C418, R419, T421, P426, R427, A428 of a Cε3 domain and residues D278 and T281 of a Cε2 domain of human IgE. Optionally, said the epitope may further comprise one or more of residues K380 and/or M430 of the Cε3 domain of human IgE and/or one or more of residues D276, V277, L279, S280, A282 and/or T298 of the Cε2 domain of human IgE. For the avoidance of doubt, the anti-IgE antibody, or antigen binding agent is specific for said epitope if it recognizes and binds to the specific human IgE structure comprising said epitope rather than to human IgE generally. 
     In a further aspect (optionally further adopting the features of the first aspect of the invention) there is provided an anti-IgE antibody, or antigen binding agent, comprising a heavy chain variable region comprising a complementarity determining region, CDR-H3, with an amino acid sequence which is Seq. ID No. 18, and a light chain variable region comprising a complementarity determining region, CDR-L1, with an amino acid sequence which is Seq. ID No. 29, wherein the light chain variable region further comprises a framework region, FR-L3, with an amino acid sequence selected from Seq. ID No. 32 which has one, two, three, four, five, six, seven or more amino acid substitutions to strengthen the interaction of the anti-IgE antibody, or antigen binding agent, with the Cε2 domain of human IgE. 
     In a further aspect (optionally further adopting the features of the previous aspects of the invention) there is provided an anti-IgE antibody, or antigen binding agent, comprising a heavy chain variable region comprising a complementarity determining region, CDR-H3, with an amino acid sequence which is Seq. ID No. 18, and a light chain variable region comprising a complementarity determining region, CDR-L1, with an amino acid sequence which is Seq. ID No. 29, wherein the light chain variable region further comprises a framework region, FR-L1, with an amino acid sequence which is Seq. ID No. 28 which has one, two, three, four, five, six, seven or more amino acid substitutions to strengthen the interaction of the anti-IgE antibody, or antigen binding agent, with the Cε2 domain of human IgE. 
     Where the CDR-H3 and CDR-L1 regions anchor and orientate the anti-IgE antibody, or antigen binding agent, on IgE Cε3 region (as per omalizumab), the change(s) to the FR-L3 and/or FR-L1 sequences allow a stronger interaction with the Cε2 domain of human IgE. The stronger interaction of the mutant relative to omalizumab or omalizumab Fab may be assessed through affinity measurements [lower K D ] (for instance as carried out by the method described in Example 6) and/or the characteristic of improved disassociation of the IgE/FcεRI complex (for instance as determined by the method described in Example 2). 
     The stronger interaction of the anti-IgE antibody, or antigen binding agent, with the Cε2 domain of human IgE may be characterised by improved functional characteristics of the anti-IgE antibody, or antigen binding agent, relative to omalizumab and/or omalizumab Fab. For instance, the anti-IgE antibody, or antigen binding agent, may be capable of disassociating human IgE from FcεRI at concentrations (or peak serum concentrations) of less than 7, 3, 1, 0.66, 0.5 or 0.3 μM (for instance as carried out by the method described in Example 2). For instance, the anti-IgE antibody, or antigen binding agent, may have an improved/stronger affinity (lower K D ) for human IgE (for instance using IgE-Fc) (for example as carried out by the method described in Example 6) relative to omalizumab and/or omalizumab Fab; and/or an improved ability to disassociate the IgE/FcεRI complex (for instance as determined by the method described in Example 2), relative to omalizumab and/or omalizumab Fab; and/or a capability of disassociating human IgE from FcεRI at concentrations (or peak serum concentrations) lower than for omalizumab and/or omalizumab Fab (for instance as determined by the method described in Example 2). By an improved K D  it is meant at least 5, 10, 20, 30, 40, or 50% lower than that of omalizumab and/or omalizumab Fab. The K D  of the anti-IgE antibody, or antigen binding agent, of the invention may be less than 2, 1.9, 1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, or 0.3 nM. By an improved ability or capability of disassociating human IgE from FcεRI it is meant at least 5, 10, 20, 30, 40, 50, or 100% improved over omalizumab and/or omalizumab Fab (for instance when measuring the % disassociation and/or apparent disassociation rate of the IgE/FcεRI complex as described in Examples 2 and 7), and/or the achievement of disassociation at a concentration where omalizumab and/or omalizumab Fab does not achieve disassociation. 
     For the avoidance of doubt, the anti-IgE antibody, or antigen binding agent, of the invention is not omalizumab or omalizumab Fab. 
     In one embodiment, the FR-L3 region is mutated, with reference to SEQ ID NO; 129, at one or more of position S60, S63, S76, S77, and/or Q79 (Kabat) to one of the other natural amino acids. 
     For instance, the FR-L3 region may be mutated at position S60 (Kabat) to one of the other natural amino acids, for instance to M, R, K, N, Q or T, in particular M. 
     For instance, the FR-L3 region may be mutated at position S63 (Kabat) to one of the other natural amino acids, for instance W or Y, in particular Y. 
     For instance, the FR-L3 region may be mutated at position S76 (Kabat) to one of the other natural amino acids, in particular N. 
     For instance, the FR-L3 region may be mutated at position S77 (Kabat) to one of the other natural amino acids, for instance R or K, in particular R. 
     For instance, the FR-L3 region may be mutated at position Q79 (Kabat) to one of the other natural amino acids, for instance R or K, in particular R. 
     For instance, the FR-L1 region may be mutated, with reference to SEQ ID NO: 20, on G16 and/or R18 (Kabat) to one of the other natural amino acids. 
     In certain embodiments, the amino acid sequence of the mutated FR-L3 region of the anti-IgE antibody, or antigen binding agent, is selected from Seq. ID No. 43-49, 60-83, 131 or 138. 
     In a further embodiment, the FR-L3 region is further mutated, with reference to SEQ ID NO: 129, at position S67 (Kabat) to one of the other natural amino acids to improve its affinity (lower K D ) for human IgE. In this case the mutation may be strengthening the interaction of the anti-IgE antibody, or antigen binding agent, for the Cε3 domain of IgE. For instance, the FR-L3 region may be mutated at position S67 (Kabat) to M (in particular), E, or D. In certain embodiments, the amino acid sequence of the mutated FR-L3 region of the anti-IgE antibody, or antigen binding agent, is selected from Seq. ID No. 53-59, 84-107, 131 or 138. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the light chain variable region further comprising a complementarity determining region, CDR-L2, with an amino acid sequence which is Seq. ID No. 31. 
     In one embodiment, the CDR-L2 region is mutated at position S52 (Kabat) to one of the other natural amino acids to improve its affinity (lower K D ) for human IgE. In this case the mutation may be strengthening the interaction of the anti-IgE antibody, or antigen binding agent, for the Cε3 domain of IgE. For instance, the CDR-L2 region may be mutated, with reference to SEQ ID NO: 129, at position S52 (Kabat) to D (in particular), E, Q or R. In certain embodiments the amino acid sequence of the mutated CDR-L2 region is selected from Seq. ID No. 50 or Seq. ID No. 51. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region further comprising a complementarity determining region, CDR-H1, with an amino acid sequence which is Seq. ID No. 14. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region further comprising a complementarity determining region, CDR-H2, with an amino acid sequence which is Seq. ID No. 16. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the light chain variable region further comprising a complementarity determining region, CDR-L3, with an amino acid sequence which is Seq. ID No. 33. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region further comprising a framework region, FR-H1, with an amino acid sequence which is Seq. ID No. 13. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region further comprising a framework region, FR-H2, with an amino acid sequence which is Seq. ID No. 15. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region further comprising a framework region, FR-H3, with an amino acid sequence which is Seq. ID No. 17. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region further comprising a framework region, FR-H4, with an amino acid sequence which is Seq. ID No. 19. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the light chain variable region further comprising a framework region, FR-L2, with an amino acid sequence which is Seq. ID No. 30. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the light chain variable region further comprising a framework region, FR-L4, with an amino acid sequence which is Seq. ID No. 34. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the light chain variable region, VL, having an amino acid sequence selected from Seq. ID No. 35, Seq. ID No. 132 or Seq ID No. 134 or Seq. ID No. 141 or Seq ID No. 144, or Seq ID No. 145 or Seq ID No. 158 or Seq ID No. 159. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region, VH, having an amino acid sequence which is Seq. ID No. 1. 
     The anti-IgE antibody, or antigen binding agent, of the invention may further comprise a light chain constant region. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have a light chain constant region which is a kappa constant region. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the light chain constant region having a mutation L154P (Kabat). 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the light chain variable region and light chain constant region, VL-CL, having an amino acid sequence selected from Seq. ID No. 39, or Seq. ID No. 41, or Seq. ID No. 117, or Seq. ID No. 119, or Seq. ID No. 125, or Seq. ID No. 127, or Seq. ID No. 136 or Seq. ID No. 143, optionally comprising a signal sequence which has an amino acid sequence which is Seq ID No. 160. 
     The anti-IgE antibody, or antigen binding agent, of the invention may further comprise a heavy chain constant region, CH1. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region and heavy chain constant region, VH-CH1, having an amino acid sequence which is Seq. ID No. 5. 
     The anti-IgE antibody, or antigen binding agent, of the invention may further comprise a heavy chain Fc region, Fc. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have an Fc which is from human IgG1 or human IgG4. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region, heavy chain constant region and heavy chain Fc region, VH-CH1-Fc, having an amino acid sequence which is Seq. ID No. 9. 
     In a further aspect of the invention there is provided an anti-IgE antibody, or antigen binding agent, comprising a heavy chain variable region comprising a complementarity determining region, CDR-H3, with an amino acid sequence which is Seq. ID No. 18, and a light chain variable region comprising a complementarity determining region, CDR-L1, with an amino acid sequence which is Seq. ID No. 29, wherein:
         a. the light chain variable region further comprises a framework region, FR-L3, with an amino acid sequence which is Seq. ID No. 32, wherein the FR-L3 region is mutated, with reference to SEQ ID NO: 129, at position S67 (Kabat) to one of the other natural amino acids to improve the affinity (lower K D ) of the anti-IgE antibody, or antigen binding agent, for human IgE; and/or   b. the light chain variable region further comprises a complementarity determining region, CDR-L2, with an amino acid sequence which is Seq. ID No. 31, wherein the CDR-L2 region is mutated, with reference to SEQ ID NO: 129, at position S52 (Kabat) to one of the other natural amino acids to improve the affinity (lower K D ) of the anti-IgE antibody, or antigen binding agent, for human IgE.       

     The present inventors, have found herein that either or both of these mutations may surprisingly improve the affinity (improved or lower K D ) of an anti-IgE antibody, or antigen binding agent, based on omalizumab or omalizumab Fab, for human IgE (for instance using IgE-Fc) (for example as carried out by the method described in Example 6). In particular, the improvement in affinity is relative to omalizumab and/or omalizumab Fab. The mutations may improve the interaction with the Cε3 domain of IgE. By an improved or lower K D  it is meant at least 5, 10, 20, 30, 40, or 50% lower than that of omalizumab and/or omalizumab Fab. The K D  of the anti-IgE antibody, or antigen binding agent, of the invention may be less than 2, 1.9, 1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, or 0.3 nM. 
     For instance, the FR-L3 region may be mutated, with reference to SEQ ID NO: 129, at position S67 (Kabat) to M (in particular), E, or D. 
     In certain embodiments, the amino acid sequence of the mutated FR-L3 region of the anti-IgE antibody, or antigen binding agent, is selected from Seq. ID No. 52-59, 84-107, 131 or 138. 
     For instance, the CDR-L2 region may be mutated, with reference to SEQ ID NO: 129, at position S52 (Kabat) to D (in particular), E, Q or R. 
     In certain embodiments, the amino acid sequence of the mutated CDR-L2 region of the anti-IgE antibody, or antigen binding agent, is selected from Seq. ID No. 50 (in particular) or Seq. ID No. 51. 
     The anti-IgE antibody, or antigen binding agent, may have the heavy chain variable region further comprising a complementarity determining region, CDR-H1, with an amino acid sequence which is Seq. ID No. 14. 
     The anti-IgE antibody, or antigen binding agent, may have the heavy chain variable region further comprising a complementarity determining region, CDR-H2, with an amino acid sequence which is Seq. ID No. 16. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region further comprising a complementarity determining region, CDR-L3, with an amino acid sequence which is Seq. ID No. 33. 
     The anti-IgE antibody, or antigen binding agent, may have the heavy chain variable region further comprising a framework region, FR-H1, with an amino acid sequence which is Seq. ID No. 13. 
     The anti-IgE antibody, or antigen binding agent, may have the heavy chain variable region further comprising a framework region, FR-H2, with an amino acid sequence which is Seq. ID No. 15. 
     The anti-IgE antibody, or antigen binding agent, may have the heavy chain variable region further comprising a framework region, FR-H3, with an amino acid sequence which is Seq. ID No. 17. 
     The anti-IgE antibody, or antigen binding agent, may have the heavy chain variable region further comprising a framework region, FR-H4, with an amino acid sequence which is Seq. ID No. 19. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region further comprising a framework region, FR-L2, with an amino acid sequence which is Seq. ID No. 30. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region further comprising a framework region, FR-L4, with an amino acid sequence which is Seq. ID No. 34. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region, VL, comprising consecutive FR-L1, CDR-L1, FR-L2, CDR-L2, FR-L3, CDR-L3, and FR-L4 regions, and having an amino acid sequence which is Seq. ID No. 20, except that the CDR-L2 region has an amino acid sequence selected from Seq. ID No. 50 (in particular) or Seq. ID No. 51. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region, VL, comprising consecutive FR-L1, CDR-L1, FR-L2, CDR-L2, FR-L3, CDR-L3, and FR-L4 regions, and having an amino acid sequence which is Seq. ID No. 20, except that the FR-L3 region has an amino acid sequence which is Seq. ID No. 52. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region, VL, comprising consecutive FR-L1, CDR-L1, FR-L2, CDR-L2, FR-L3, CDR-L3, and FR-L4 regions, and having an amino acid sequence which is Seq. ID No. 20, except that the CDR-L2 region has an amino acid sequence selected from Seq. ID No. 50 (in particular) or Seq. ID No. 51, and the FR-L3 region has an amino acid sequence which is selected from Seq. ID No. 52, 131 or 138. 
     The anti-IgE antibody, or antigen binding agent, of the invention may have the heavy chain variable region, VH, having an amino acid sequence which is Seq. ID No. 1. 
     The anti-IgE antibody, or antigen binding agent, may further comprise a light chain constant region. 
     The anti-IgE antibody, or antigen binding agent, may have a light chain constant region which is a kappa constant region. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region and light chain constant region, VL-CL, having an amino acid sequence which is Seq. ID No. 24, except that the CDR-L2 region has an amino acid sequence selected from Seq. ID No. 50 (in particular) or Seq. ID No. 51. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region and light chain constant region, VL-CL, having an amino acid sequence which is Seq. ID No. 24, except that the FR-L3 region has an amino acid sequence which is Seq. ID No. 52. 
     The anti-IgE antibody, or antigen binding agent, may have the light chain variable region and light chain constant region, VL-CL, having an amino acid sequence which is Seq. ID No. 24, except that the CDR-L2 region has an amino acid sequence selected from Seq. ID No. 50 (in particular) or Seq. ID No. 51, and the FR-L3 region has an amino acid sequence which is selected from Seq. ID No. 52, 131 or 138. 
     In a further aspect, the invention provides for an anti-IgE antibody, or antigen binding agent, comprising a heavy chain variable region and a light chain variable region, wherein:
         a. the heavy chain variable region comprises a CDR-H1 with an amino acid sequence which is Seq ID No. 14, a CDR-H2 with an amino acid sequence which is Seq ID No. 16 and a CDR-H3 with an amino acid sequence which is Seq ID No. 18 and the light chain variable region comprises CDR-L1 with an amino acid sequence which is Seq ID No. 29, a CDR-L2 with an amino acid sequence which is Seq ID No. 50, a CDR-L3 with an amino acid sequence which is Seq ID No. 33 and a framework region FW-L3 with an amino acid sequence which is Seq ID No. 131 or 138; or   b. the heavy chain variable region comprises an amino acid sequence which is Seq ID No. 1 and the light chain variable region comprises an amino acid sequence selected from Seq ID No. 132 or 139.
 
In one embodiment, the anti-IgE antibody, or antigen binding agent may further comprise a light chain constant region, wherein the light chain variable region and the light chain constant region VL-CL have an amino acid sequence selected from Seq ID No. 136 or 143, optionally comprising a signal sequence which has an amino acid sequence which is Seq ID No. 160.
       

     The anti-IgE antibody, or antigen binding agent, in all embodiments as described herein may further comprise a heavy chain constant region, CH1. 
     The anti-IgE antibody, or antigen binding agent, may have the heavy chain variable region and heavy chain constant region, VH-CH1, having an amino acid sequence which is Seq. ID No. 5. 
     The anti-IgE antibody, or antigen binding agent, may further comprise a heavy chain Fc region, Fc. 
     The anti-IgE antibody, or antigen binding agent, may have the Fc from human IgG1 or human IgG4. 
     The anti-IgE antibody, or antigen binding agent, may have the heavy chain variable region, heavy chain constant region and heavy chain Fc region, VH-CH1-Fc, having an amino acid sequence which is Seq. ID No. 9. 
     The anti-IgE antibody, or antigen binding agent, of all aspects of the invention may be selected from the group consisting of: a complete antibody molecule having full length heavy and light chains, or a fragment thereof. 
     The anti-IgE antibody, or antigen binding agent, of the invention may be elected from the group consisting of: a Fab fragment, modified Fab′ fragment, Fab′ fragment, F(ab′) 2  fragment, Fv, scFv, scab, a diabody, bispecific antibody, triabody, FabFv, Fab-Fv-Fv, tribody, or a (Fab-Fv) 2 -Fc. Without being bound by theory, an anti-IgE antibody, or antigen binding agent, of the invention may have less anaphylaxis risk associated with it if it has only one rather than multiple anti-IgE antigen binding sites. 
     In one embodiment, the anti-IgE antibody is a Fab fragment linked directly or via a linker to a scFv that binds to a serum carrier protein, such as human serum albumin.
 
In one embodiment the scFv may comprise a heavy chain variable region and a light chain variable region, preferably linked via a linker having Seq. ID No. 151, wherein the heavy chain variable region comprises a CDR-H1 with an amino acid sequence which is Seq ID No. 152, a CDR-H2 with an amino acid sequence which is Seq ID No. 153 and a CDR-H3 with an amino acid sequence which is Seq ID No. 154 and the light chain variable region comprises CDR-L1 with an amino acid sequence which is Seq ID No. 155, a CDR-L2 with an amino acid sequence which is Seq ID No. 156, a CDR-L3 with an amino acid sequence which is Seq ID No. 157.
 
In one embodiment the scFv has an amino acid sequence which is Seq ID No. 150. In one preferred embodiment, the Fab fragment comprises a heavy chain variable region and a light chain variable region, wherein:
         a. the heavy chain variable region comprises a CDR-H1 with an amino acid sequence which is Seq ID No. 14, a CDR-H2 with an amino acid sequence which is Seq ID No. 16 and a CDR-H3 with an amino acid sequence which is Seq ID No. 18 and the light chain variable region comprises CDR-L1 with an amino acid sequence which is Seq ID No. 29, a CDR-L2 with an amino acid sequence which is Seq ID No. 50, a CDR-L3 with an amino acid sequence which is Seq ID No. 33 and a framework region FW-L3 with an amino acid sequence which is Seq ID No. 131 or 138; or   b. the heavy chain variable region comprises an amino acid sequence which is Seq ID No. 1 and the light chain variable region comprises an amino acid sequence selected from Seq ID No. 132 or 139.
 
In another embodiment, the Fab fragment further comprises a heavy chain constant region and a light chain constant region, wherein the heavy chain variable region and the heavy chain constant region VL-CH1 has an amino acid sequence which is Seq ID No. 5 and wherein the light chain variable region and the light chain constant region VL-CL has an amino acid sequence selected from Seq ID No. 136 or 143, optionally comprising a signal sequence which has an amino acid sequence which is Seq ID No. 160.
 
In another embodiment scFv is linked to the CH1 of the Fab fragment via a linker having amino acid sequence which is Seq ID No. 149.
 
In one embodiment, the heavy chain variable region and the heavy chain constant region, the linker and the scFv has an amino acid sequence which is Seq ID No. 147, optionally comprising a signal sequence which has an amino acid sequence which is Seq ID No. 160.
 
In one other embodiment the heavy chain of the Fab fragment linked to the scFv with Seq. ID No. 147 is paired with a light chain variable and constant region which has Seq ID No. 136 or 143.
       

     The anti-IgE antibody, or antigen binding agent, of the invention may have an effector or a reporter molecule attached to it. 
     The anti-IgE antibody, or antigen binding agent, of the invention may be glycosylated (for instance within the Fc domain) and/or may be conjugated to a polymer selected from starch, albumin, and polyethylene glycol (PEG). In one embodiment, conjugated PEG may have a molecular weight in the range 5 to 50 kDa. 
     The anti-IgE antibody, or antigen binding agent, of the invention may be humanized. 
     A further aspect of the invention relates to an isolated DNA sequence encoding the heavy and/or light chain(s) of the anti-IgE antibody, or antigen binding agent, of the invention. Further provided is a cloning or expression vector comprising one or more DNA sequences of the invention. For instance, a cloning or expression vector may comprise one or more DNA sequences selected from Seq. ID No. 36, Seq. ID No. 38, Seq. ID No. 40, or Seq. ID No. 42, or Seq. ID No. 133, or Seq ID No. 135, or Seq ID No. 137, or Seq ID No. 140, or Seq. ID No. 142, or Seq ID No. 144 and, optionally, may further comprise one or more DNA sequences selected from Seq. ID No. 2, Seq. ID No. 4, Seq. ID No. 6, Seq. ID No. 8, Seq. ID No. 10, or Seq. ID No. 12 or Seq. ID No. 148. 
     A further aspect of the invention is a host cell comprising one or more cloning or expression vectors of the invention. The host cell of the invention may optionally further comprise one or more cloning or expression vectors comprising one or more DNA sequences selected from Seq. ID No. 2, Seq. ID No. 4, Seq. ID No. 6, Seq. ID No. 8, Seq. ID No. 10, or Seq. ID No. 12 or Seq ID No. 148. 
     A process for the production of the anti-IgE antibody, or antigen binding agent, of the invention is also provided, comprising culturing the host cell of the invention and isolating the anti-IgE antibody, or antigen binding agent. 
     A further aspect relates to a pharmaceutical composition comprising the anti-IgE antibody, or antigen binding agent, of the invention, in combination with one or more of a pharmaceutically acceptable excipient, diluent or carrier. Suitably, the anti-IgE antibody, or antigen binding agent, of the invention is present at a dose of 50-200, preferably around or exactly 150 mg per mL diluent. In certain embodiments, the excipient comprises one or both of L-arginine, L-histidine. The excipient may separately or in combination comprise Polysorbate 20. The diluent may be water or an aqueous isotonic solution. 
     The pharmaceutical composition of the invention may be carried within a sterile vial as a powder for reconstitution prior to subcutaneous administration, or within a sterile syringe for its immediate subcutaneous administration. 
     The pharmaceutical composition of the invention may contain a total dose of anti-IgE antibody, or antigen binding agent, of 75-600 mg—for instance around or exactly 100 or 150 mg. 
     The pharmaceutical composition of the invention may additionally comprise other active ingredients either contained together with the anti-IgE antibody, or antigen binding agent, or for separate coadministration with the anti-IgE antibody, or antigen binding agent. For instance, the pharmaceutical composition of the invention may be used in the context of allergy-based specific immunotherapy, where the anti-IgE antibody, or antigen binding agent, of the invention is separately coadministered (but may be co-packaged) with an allergen. The pharmaceutical composition of the invention may thus be for use in allergy-based specific immunotherapy, where the patient receives the pharmaceutical composition of the invention 7, 6, 5, 4, 3, 2, or 1 days before (or on the same day) as the therapeutic allergen. 
     The anti-IgE antibody, or antigen binding agent, or composition, of the invention may be for use as a medicament. 
     The anti-IgE antibody, or antigen binding agent, or composition, of the invention may be for use in the treatment or prevention of disease. 
     The anti-IgE antibody, or antigen binding agent, or composition, of the invention may be for use in the treatment or prevention of disorders associated with the complex of human IgE and FcεRI. 
     The anti-IgE antibody, or antigen binding agent, or composition, of the invention may be for use in the treatment or prevention of disorders through the disassociation of the complex of human IgE and FcεRI and the binding of human IgE by the anti-IgE antibody, or antigen binding agent. 
     The anti-IgE antibody, or antigen binding agent, or composition, of the invention may be for use in the treatment or prevention of one or more of: allergy; allergic asthma; severe asthma; moderate asthma; chronic spontaneous urticaria; chronic idiopathic urticaria; perennial allergic rhinitis; seasonal allergic rhinitis; acute asthma exacerbations; acute bronchospasm; status asthmaticus; hyper IgE syndrome; allergic bronchopulmonary aspergillosis; prevention of anaphylactic reactions; food allergy; atopic dermatitis; allergic rhinitis; bee venom sensitivity; idiopathic anaphylaxis; peanut allergy; latex allergy; inflammatory skin diseases; urticaria (solar, cold-induced, local heat-induced, and/or delayed pressure-induced); cutaneous mastocytosis; systemic mastocytosis; eosinophil-associated gastrointestinal disorder; bullous pemphigoid; interstitial cystitis; nasal polyps; idiopathic angioedema; or non-allergic asthma. 
     Further provided is a method for the treatment or prevention of a disease in a human subject, the method comprising administering to the subject an effective amount of the anti-IgE antibody, or antigen binding agent, or a composition of the invention. The method may be for the treatment or prevention of disorders associated with the complex of human IgE and FcεRI. The method of the invention may treat or prevent disease through the disassociation of the complex of human IgE and FcεRI and the binding of human IgE by the anti-IgE antibody, or antigen binding agent of the invention. 
     The method of the invention may be for the treatment or prevention of one or more of: allergy; allergic asthma; severe asthma; moderate asthma; chronic spontaneous urticaria; chronic idiopathic urticaria; perennial allergic rhinitis; seasonal allergic rhinitis; acute asthma exacerbations; acute bronchospasm; status asthmaticus; hyper IgE syndrome; allergic bronchopulmonary aspergillosis; prevention of anaphylactic reactions; food allergy; atopic dermatitis; allergic rhinitis; bee venom sensitivity; idiopathic anaphylaxis; peanut allergy; latex allergy; inflammatory skin diseases; urticaria (solar, cold-induced, local heat-induced, and/or delayed pressure-induced); cutaneous mastocytosis; systemic mastocytosis; eosinophil-associated gastrointestinal disorder; bullous pemphigoid; interstitial cystitis; nasal polyps; idiopathic angioedema; or non-allergic asthma. 
     In the present invention, it has been elucidated that an antibody or an antigen binding agent against a first polypeptide, which polypeptide elicits its physiological response by virtue of binding to a second polypeptide (such as a receptor), is capable of binding to both the free and bound first polypeptide, stabilising a conformation of such first polypeptide. Such stabilised conformation has a binding affinity for the second polypeptide weaker than in the absence of the antibody or antigen binding agent hence, triggering faster dissociation of the first polypeptide from the second polypeptide. 
     In this respect, the invention provides a further aspect which relates to an antibody or an antigen binding agent, capable of binding free and FcεRI bound human IgE and stabilising a conformation of IgE. When the IgE is in such conformation, it has a binding affinity for FcεRI weaker than in the absence of the antibody or antigen binding agent and wherein the FcεRI bound human IgE dissociates from FcεRI. Optionally, when the IgE is in such conformation, the IgE has a lower binding affinity for omalizumab or a fragment thereof than the antibody or antigen binding agent of the invention. For example, the antibody or antigen binding agent is an antibody as described herein. 
     In a further aspect, the invention relates to a process for selecting such antibodies or antigen binding agents as described herein. The process comprises:
         a. Contacting a test antibody or antigen binding agent with a sample comprising human IgE bound to human FcεRI;   b. Measuring the constant of dissociation of the test antibody or antigen binding agent for dissociation the human IgE from human FcεRI;   c. Comparing the constant of dissociation as measured in step b) with the constant of dissociation of omalizumab or a fragment thereof for dissociating the human IgE from to human FcεRI;   d. Selecting the antibody or antigen binding agent if said antibody or antigen binding agent dissociates the IgE from FcεRI faster than omalizumab or a fragment thereof.
 
Alternatively, the process for selecting antibodies or antigen binding agents according to the invention comprises:
   a. Contacting a test antibody or antigen binding agent with a sample comprising human IgE bound to human FcεRI;   b. Measuring the binding affinity of the test antibody or antigen binding agent for human IgE from human FcεRI;   c. Comparing the binding affinity as measured in step b) with the binding affinity of human IgE for FcεRI;   d. Selecting the antibody or antigen binding agent if said antibody or antigen binding agent has a higher binding affinity for IgE than the IgE for FcεRI.
 
Optionally, the selected antibodies or antigen binding agents cause the IgE, whilst still bound to FcεRI, to adopt a conformation wherein the IgE in said stabilised conformation may dissociate from FcεRI faster than the IgE bound to FcεRI in the presence of omalizumab or a fragment thereof; and/or may have a binding affinity higher for the antibody or antigen binding agent than for the FcεRI.
 
In a final aspect, the present invention relates to specific antibodies or antigen binding agent comprising:
   a. a heavy chain variable region comprising Seq. ID No.: 1 and a light chain variable region comprising:
           i. Seq. ID No.: 109; or   ii. Seq. ID No.: 113; or   iii. Seq. ID No.: 121; or   iv. Seq. ID No.: 132; or   v. Seq. ID No.: 139; or   
           b. Seq. ID No.: 5 and
           i. Seq. ID No.: 24, wherein S77 and S79 are replaced by Q;   ii. Seq. ID No.: 117 or   iii. Seq. ID No.: 125; or   iv. Seq. ID No.: 136; or   v. Seq. ID No.: 143.
 
In one embodiment of this last aspect of the invention, the anti-IgE antibody or antigen binding agent contacts or, contacts and is specific for, an epitope comprising, with reference to SEQ ID NO: 108, residues T373, W374, S375, R376, A377, S378, G379, P381, Q417, C418, R419, T421, P426, R427, A428 of a Cε3 domain and residues D278 and T281 of a Cε2 domain of human IgE.
   
               

    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       References and SEQ IDs are found in the Examples referring to the Figures. 
         FIG. 1 . omalizumab Fab3 contains three point mutations. omalizumab Fab3 is derived from omalizumab, and contains three point mutations distal to the antigen binding CDRs, two in the VL domain framework region (Ser81 Arg, Gln83Arg) and one in the Cκ domain (Leu158Pro). The heavy and light chains are colored white and blue, respectively. The mutated residues are colored red, and CRDL1 in green, to indicate the orientation of the Fab. 
         FIG. 2 . Overall structure of IgE-Fc in complex with omalizumab Fab3. (A) omalizumab Fab3 binds to IgE-Fc with 2:1 stoichiometry. Fab 1  (green) engages IgE-Fc chain B (pink) exclusively through the Cε3 domain. Fab 2  (blue) interacts with IgE-Fc chain A (yellow) through the Cε3 domain and forms a minor interaction with the Cε2 domain from IgE-Fc chain B (pink). (B) The two Fabs form a pseudo-symmetric complex about the two-fold axis of the Fcε3-4 region. For clarity, the Cε2 domains are not shown. (C) IgE-Fc is asymmetrically bent in the omalizumab Fab3 complex. The Cε2 domain from chain B (pink) contacts Fab 2  (blue). 
         FIG. 3 . Interface between omalizumab Fab3 and IgE-Fc. The interface between omalizumab Fab3 Fab 2  (heavy and light chains colored in green and yellow, respectively) and the Cε3 domain from IgE-Fc (pink) is shown. omalizumab Fab3 and Cε3 domain residue labels are colored blue and black, respectively. The interface comprises hydrogen bonds and van der Waals interactions. A notable feature of the interface is a cation-π interaction between Arg419 (Cε3 domain) and Phe103 (omalizumab Fab3 CDRH3). The Phe103 side chain is mostly buried in a pocket created by Thr373, Trp374, Ser375, G1n417 and Arg419 (Cε3 domain). 
         FIG. 4 . omalizumab Fab3 and DARPin E2_79 bind to an overlapping interface. omalizumab Fab3 and DARPin E2_79 20  both bind to the Cε3 domain. IgE-Fc residues which only form part of the omalizumab Fab3 interface are colored orange, while those which only form part of the DARPin E2_79 interface, which includes part of the Cε3-Cε4 linker, are colored in blue. IgE-Fc residues colored in pink, which include Arg419 and Met430, are common to both omalizumab Fab3 and DARPin E2_79 interfaces. 
         FIG. 5 . Conformational flexibility in IgE-Fc. (A) Side view of free IgE-Fc 8  showing its acute, asymmetric bend. (B) Front view of free IgE-Fc (90° anti-clockwise rotation from the view shown in (A). (C) Side view of IgE-Fc from the omalizumab Fab3 complex, revealing a partially bent conformation. (D) Front view of IgE-Fc in the omalizumab Fab3 complex (90° anti-clockwise rotation from the view shown in (C). (E) Side view of fully extended IgE-Fc captured by an anti-IgE-Fc Fab (omalizumab Fab3) 16 . (F) Front view of extended IgE-Fc (90° anti-clockwise rotation from the view shown in (E). 
         FIG. 6 . Conformational flexibility in IgE-Fc. Flexibility of IgE-Fc, and unbending from the bent to a fully extended conformation, was previously explored by molecular dynamics 16 . IgE-Fc unbending is represented as a free energy surface, as previously described 16 . (A) Extended conformation of IgE-Fc captured in the crystal structure of the omalizumab Fab3/IgE-Fc complex 16 . (B) Partially bent IgE-Fc conformation observed in the crystal structure of the omalizumab Fab3/IgE-Fc complex. (C) Bent conformation of free IgE-Fc 7,8 . The bent conformation of IgE-Fc occupies the lowest energy basin, while the partially bent conformation observed in the omalizumab Fab3/IgE-Fc complex occupies a clearly distinct energy basin (B). 
         FIG. 7 . Disruption of the interaction between IgE-Fc and FcεRI. In the omalizumab Fab3 complex, the Cε3 domains adopt the most open conformation reported thus far for IgE-Fc, which precludes engagement with FcεRIα. The structure of IgE-Fc in complex with FcεRIα 8  is colored yellow, and the two sub-sites of receptor engagement are indicated. The structure of omalizumab Fab3 in complex with IgE-Fc was superposed on the Cε4 domains, and the Cε3 domains are colored blue. Positions of His424 and Pro426 in the two structures are indicated, to highlight the different positions adopted by the Cε3 domains. 
         FIG. 8 . Disruption of the interaction between IgE-Fc and CD23. (A) Cε3 domain residues which are common to both omalizumab Fab3 and CD23 interfaces are colored pink. (B) Superposition of the Cε3 domains (dark gray) from the omalizumab Fab3/IgE-Fc complex and CD23/Fcε3-4 complex 11  reveal clashes between CD23 (yellow) and omalizumab Fab3 (pink). 
         FIG. 9 . Interaction studies of omalizumab Fab3 with IgE-Fc. (A) Binding of omalizumab Fab3 to IgE-Fc captured via a C-terminal His-tag; omalizumab Fab3 was flowed over IgE-Fc at the following concentrations 100 nM (black), 50 nM (red), 25 nM (green), 12.5 nM (blue), 6.2 nM (cyan), 3.1 nM (purple), 1.6 nM (magenta) and 0.8 nM (dark red). Standard double referencing methods were employed 36 ; each concentration was run in duplicate. (B) Binding of the second omalizumab Fab3 binding site was characterized using an SPR sandwich binding experiment. IgE-Fc was captured on an omalizumab Fab3 surface, and then a second omalizumab Fab3 molecule was added to the IgE-Fc/omalizumab Fab3 complex at concentrations of 1000 nM (black), 500 nM (red), 250 nM (green), 125 nM (blue), 62.5 nM (cyan), 31.2 nM (purple), 15.6 nM (magenta), 7.8 nM (dark red) and 0 nM (navy). (C) A comparison of the ability of omalizumab Fab3 to bind to IgE-Fc captured by a C-terminal His-tag (red) and IgE-Fc captured by binding to FcεRIα (blue); a two-fold dilution series was tested for each, with a highest concentration of 1000 nM. The inset shows that omalizumab Fab3 can still bind to the IgE-Fc/FcεRIα complex, but with a low B. value. (D) Accelerated dissociation of the IgE-Fc/FcεRIα complex mediated by increasing concentrations of omalizumab Fab3. The 1:1 IgE-Fc/FcεRIα complex was first established by capturing IgE-Fc on immobilized FcεRIα and then binding omalizumab Fab3 at the following concentrations: 5000 nM (magenta), 1000 nM (purple), 200 nM (cyan), 40 nM (blue), 8 nM (green), 1.6 nM (red) and 0 nM (black). The inset shows a magnification of the accelerated dissociation process. All concentrations were run in duplicate. All binding experiments were performed at 25° C., except those characterizing the second omalizumab Fab3 binding site ( FIG. 4B ), which were done at 5° C. to minimize allosteric communication between the two sites. 
         FIG. 10 . Analysis of direct binding, competition experiments and accelerated dissociation. Direct binding was measured for IgE-Fc to immobilized omalizumab Fab3 (A), omalizumab Fab (B), and intact omalizumab (C). Fabs or intact antibody were covalently immobilized at low density using an amine coupling kit (GE Healthcare); IgE-Fc was flowed over these surfaces at a variety of concentrations, using a two-fold dilution series with a highest concentration of 100 nM. All concentrations were run in duplicate. (D) TR-FRET competition binding experiments between omalizumab Fab3 and ay-fusion protein for IgE-Fc. Binding between terbium-labeled ay-fusion protein and Alexa Fluor 647-labeled IgE-Fc was measured with increasing concentrations of unlabeled omalizumab Fab3 as inhibitor: 0 μM (black), 2.5 nM (blue), 5 nM (green), 10 nM (magenta), 20 nM (red). As an inhibitor, omalizumab Fab affects both the apparent K D  and B max  of the interaction between IgE-Fc and ay-fusion protein, indicating some allosteric inhibition properties. (E) Comparison of the accelerated dissociation of the IgE-Fc/sFcεRIα complex mediated by intact omalizumab (black), omalizumab Fab (red) or omalizumab Fab3 (blue), each at a concentration of 5 μM. 
         FIG. 11 . Representative electron density map. A stereoview of the 2F o -F c  electron density map, contoured at 1.1σ, is shown for a portion of the chain A Cε3 domain, and covalently N-linked oligosaccharide moiety at Asn394. 
         FIG. 12 . Biacore sensorgram of the dissociation of IgE-Fc from immobilized sFcεRIα. Dissociation was monitored in the presence of running buffer (solid line) or IgE binding partners (all other sensorgrams). Assay performed as described in Assay Method (1) Example 2. 
         FIG. 13 . Biacore sensorgram of the dissociation of IgE-Fc from immobilized sFcεRIα. Dissociation was monitored in the presence of control supernatant (solid line) or IgE binding partners (all other sensorgrams). Assay performed as described in Assay Method (2) Example 2. 
         FIG. 14 . Analysis of dissociation of Alexa488 labelled IgE-Fc from the surface of RBL-SX38 cells. Measured binding data normalized to 100% at t=0 and dissociation data plotted as the change in proportion of IgE-Fc remaining bound as a function of time. 
         FIG. 15 . Analysis of the effect of therapeutic dosing of wild type omalizumab Fab and omalizumab Fab3 on the 72 hour PCA model. 
         FIG. 16 . Residues 224 to 547 of wild-type human IgE-Fc sequence (as shown in SEQ ID NO: 108) with residues 224 and 547 shown in bold. The numbering is according to Dorrington and Bennich (1978) Immunol. Rev. 41:3-25, whereby the L (Leu; leucine) after L253 is numbered as L235a (boxed) and the subsequent residue is C254. The remaining residues are numbered consecutively with no further additions. The epitope residues are shown with an asterisk (*). 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Antibody amino acid numbering herein will either be from the consecutive amino acid sequence of an antibody (for instance omalizumab comprising VII sequence of Seq. ID No. 1 and VL sequence of Seq. ID No. 20 or SEQ ID NO: 129)—the so called “pdb” numbering—or may utilize the common Kabat numbering scheme. Where the common immunoglobulin parts (CDRs—complementarity determining regions, or FRs—framework regions) of a VH or VL sequence are described, they are linked in the standard order (VH=FR-H1.CDR-H1.FR-H2.CDR-H2.FR-H3.CDR-H3.FR-H4; VL=FR-L1.CDR-L1.FR-L2.CDR-L2.FR-L3.CDR-L3.FR-L4). For omalizumab “pdb” numbering of VH (Seq. ID No. 1) parts is: FR-H1 (amino acids 1-25), CDR-H1 (26-36), FR-H2 (37-50), CDR-H2 (51-66), FR-H3 (67-98), CDR-H3 (99-110), FR-H4 (111-121); whereas Kabat numbering is: FR-H1 (amino acids 1-25), CDR-H1 (26-35), FR-H2 (36-49), CDR-H2 (50-65), FR-H3 (66-94), CDR-H3 (95-102), FR-H4 (103-113). For omalizumab “pdb” numbering of VL (Seq. ID No. 20) parts is: FR-L1 (amino acids 1-23), CDR-L1 (24-38), FR-L2 (39-53), CDR-L2 (54-60), FR-L3 (61-92), CDR-L3 (93-101), FR-L4 (102-111); whereas Kabat numbering is: FR-L1 (amino acids 1-23), CDR-L1 (24-34), FR-L2 (35-49), CDR-L2 (50-56), FR-L3 (57-88), CDR-L3 (89-97), FR-LA (98-107). 
     IgE antibody numbering is as reported by Dorrington &amp; Bennich (1978) Immunol. Rev. 41:3-25). Thus, the IgE-Fc polypeptides used in this invention (see Seq. ID No. 108) are from V224-K547 (including a C225A mutation). As shown in  FIG. 16 , the numbering followed is according to Dorrington and Bennich (1978) Immunol. Rev. 41:3-25 where the L (Leu, leucine) following position 253 is numbered L253a and the remaining residues are numbered consecutively from L253a as C254 etc. In the crystallography experiments the following mutations were also inserted into the IgE-Fc to simplify the glycosylation pattern: N265Q &amp; N371Q. The Cε2 region of IgE-Fc is generally accepted to occupy the sequence 5226-D330. Reference to IgE herein may be a reference to human IgE (and vice versa), and may also constitute a reference to IgE-Fc in the context of the assays and methods described herein. The sequence of the Fab arms of the full length human IgE antibody are not included in this description as they are not present in the crystal structures. 
     Herein reference to “omalizumab” is a reference to the commercially-sold Xolair® product; or to an IgG full-length antibody comprising a heavy chain comprising the VH amino acid sequence which is Seq. ID No. 1, and a light chain comprising the VL amino acid sequence which is Seq. ID No. 20; or to an IgG full-length antibody comprising a heavy chain comprising the VH-CH1 amino acid sequence which is Seq. ID No. 5, and a light chain comprising the VL-CL amino acid sequence which is Seq. ID No. 24; or to an IgG full-length antibody comprising a heavy chain comprising the VH-CH1-Fc amino acid sequence which is Seq. ID No. 9, and a light chain comprising the VL-CL amino acid sequence which is Seq. ID No. 24. Reference to “omalizumab Fab” is a reference to a Fab fragment comprising a heavy chain comprising the VH amino acid sequence which is Seq. ID No. 1, and a light chain comprising the VL amino acid sequence which is Seq. ID No. 20; or (in particular) to a Fab fragment comprising a heavy chain comprising the VH-CH1 amino acid sequence which is Seq. ID No. 5, and a light chain comprising the VL-CL amino acid sequence which is Seq. ID No. 24. 
     General Definitions 
     Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described herein. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In addition, the materials, methods, and examples are illustrative only and are not intended to be limiting. 
     Unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular. In this application, the use of “or” means “and/or” unless stated otherwise. Furthermore, the use of the term “including”, as well as other forms, such as “includes” and “included”, is not limiting. Also, terms such as “element” or “component” encompass both elements and components comprising one unit and elements and components that comprise more than one subunit unless specifically stated otherwise. 
     Generally, nomenclatures used in connection with, and techniques of, cell and tissue culture, molecular biology, immunology, microbiology, genetics, and protein and nucleic acid chemistry and hybridization described herein are those well-known and commonly used in the art. The methods and techniques of the present invention are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification unless otherwise indicated. Enzymatic reactions and purification techniques may be performed according to manufacturer&#39;s specifications, as commonly accomplished in the art or as described herein. The nomenclatures used in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal, and pharmaceutical chemistry described herein are those well-known and commonly used in the art. Standard techniques are used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients. 
     That the present invention may be more readily understood, selected terms are defined below. 
     The term “host” as used herein, typically refers to a human subject, and in particular where a human or humanized framework is used as an acceptor structure. Where another host is treated, it is understood by those of skill in the art that the antibody or antigen binding agent may need to be tailored to that host to avoid rejection or to make more compatible. It is known how to use the CDRs in the present invention and engineer them into the proper framework or peptide sequence for desired delivery and function for a range of hosts. Other hosts may include other mammals or vertebrate species. The term “host,” therefore, can alternatively refer to animals such as mice, monkeys, dogs, pigs, rabbits, domesticated swine (pigs and hogs), ruminants, equine, poultry, felines, murines, bovines, canines, and the like, where the antibody or antigen binding agent, if necessary is suitably designed for compatibility with the host. 
     The term “polypeptide” as used herein, refers to any polymeric chain of amino acids. The terms “peptide” and “protein” are used interchangeably with the term polypeptide and also refer to a polymeric chain of amino acids. The term “polypeptide” encompasses native or artificial proteins, protein fragments, and polypeptide analogs of a protein sequence. A polypeptide may be monomeric or polymeric. 
     The term “recovering” as used herein, refers to the process of rendering a chemical species such as a polypeptide substantially free of naturally associated components by isolation, e.g., using protein purification techniques well known in the art. 
     The terms “specific binding” or “specifically binding”, as used herein, in reference to the interaction of an antibody, a protein, or a peptide with a second chemical species, mean that the interaction is dependent upon the presence of a particular structure (e.g., an “antigenic determinant” or “epitope” as defined below) on the chemical species; for example, an antibody recognizes and binds to a specific protein structure rather than to proteins generally. If an antibody is specific for epitope “A”, the presence of a molecule containing epitope A (or free, unlabeled A), in a reaction containing labeled “A” and the antibody, will reduce the amount of labeled A bound to the antibody. Where an epitope of the invention is mentioned herein, the anti-IgE antibody, or antigen binding agent, of the invention is specific for said epitope. 
     The term “antibody”, as used herein, broadly refers to any immunoglobulin (Ig) molecule comprised of four polypeptide chains, two heavy (H) chains and two light (L) chains, or any functional fragment, mutant, variant, or derivation thereof, which retains at least some portion of the epitope binding features of an Ig molecule allowing it to specifically bind to IgE. Such mutant, variant, or derivative antibody formats are known in the art and described below. Non limiting embodiments of which are discussed below. An antibody is said to be “capable of binding” a molecule (or epitope) if it is capable of specifically reacting with the molecule (or epitope) to thereby bind the molecule (or epitope) to the antibody. 
     A “monoclonal antibody” as used herein is intended to refer to a preparation of antibody molecules, which share a common heavy chain and common light chain amino acid sequence, or any functional fragment, mutant, variant, or derivation thereof which retains at least the light chain epitope binding features of an Ig molecule, in contrast with “polyclonal” antibody preparations that contain a mixture of different antibodies. Monoclonal antibodies can be generated by several known technologies like phage, bacteria, yeast or ribosomal display, as well as classical methods exemplified by hybridoma-derived antibodies (e.g., an antibody secreted by a hybridoma prepared by hybridoma technology, such as the standard Kohler and Milstein hybridoma methodology ((1975) Nature 256:495-497). 
     In a full-length antibody, each heavy chain is comprised of a heavy chain variable region (abbreviated herein as HCVR or VH) and a heavy chain constant region (CH). The heavy chain constant region is comprised of four domains—either CH1, Hinge, CH2, and CH3 (heavy chains γ, α and δ), or CH1, CH2, CH3, and CH4 (heavy chains μ and ε). Each light chain is comprised of a light chain variable region (abbreviated herein as LCVR or VL) and a light chain constant region (CL). The light chain constant region is comprised of one domain, CL. The VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR). Each VH and VL is composed of three CDRs and four FRs, arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. Immunoglobulin molecules can be of any type (e.g., IgG, IgE, IgM, IgD, IgA and IgY), class (e.g., IgG1, IgG2, IgG3, IgG4, IgA1 and IgA2) or subclass. 
     The term “antigen binding agent” as used herein, refers to one or more fragments or portions of an antibody that retain the ability to specifically bind to an antigen (e.g., IgE), or synthetic modifications of antibody fragments that retain the desired binding ability to the antigen. It has been shown that the antigen-binding function of an antibody can be performed by fragments or certain portions of a full-length antibody, or modifications thereof. Embodiments include bispecific, dual specific and multi-specific formats which may specifically bind to two or more different antigens or to several epitopes or discontinuous epitope regions of an antigen. Non limiting examples of antigen binding agents include (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL, and CH1 domains; (ii) a F(ab′)2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CH1 domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v) a dAb fragment (Ward et al., (1989) Nature 341:544-546, Winter et al., PCT publication WO 90/05144 A1 herein incorporated by reference), which comprises a single variable domain; (vi) an isolated complementarity determining region (CDR), (vii) fusions of antibody fragments such as those that are immunoglobulin in character, for example, diabodies, scab, bispecific, triabody, Fab-Fv, Fab-Fv-Fv, tribody, (Fab-Fv)2-Fc, and (viii) antibody portions such as CDRs or antibody loops grafted onto non-immunoglobulin frameworks such as fibronectin or leucine zippers (see Binz et al. (2005) Nat. Biotech. 23:1257-1268, incorporated herein). Furthermore, although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they can be joined, using recombinant or other methods, by a synthetic or naturally occurring linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see e.g., Bird et al. (1988) Science 242:423-426; and Huston et al. (1988) Proc. Natl. Acad. Sci. USA 85:5879-5883). Such single chain antibodies are also intended to be encompassed within the term antigen binding agent. Other forms of single chain antibodies, such as diabodies are also encompassed. Diabodies are bivalent, bispecific antibodies in which VH and VL domains are expressed on a single polypeptide chain, but using a linker that is too short to allow for pairing between the two domains on the same chain, thereby forcing the domains to pair with complementary domains of another chain and creating two antigen binding sites (see e.g., Holliger, P., et al. (1993) Proc. Natl. Acad. Sci. USA 90:6444-6448; Poljak, R. J., et al. (1994) Structure 2:1121-1123). Such antibody binding portions are known in the art (Kontermann and Dubel eds., Antibody Engineering (2001) Springer-Verlag. New York. 790 pp. (ISBN 3-540-41354-5). 
     The term “antibody construct” as used herein refers to a polypeptide comprising one or more of the antigen binding portions of the invention linked to a linker polypeptide or an immunoglobulin constant domain. Linker polypeptides comprise two or more amino acid residues joined by peptide bonds and are used to link one or more antigen binding portions. Such linker polypeptides are well known in the art (see e.g., Holliger, P., et al. (1993) Proc. Natl. Acad. Sci. USA 90:6444-6448; Poljak, R. J., et al. (1994) Structure 2:1121-1123). An immunoglobulin constant domain refers to a heavy or light chain constant domain, for example a human IgA, IgD, IgE, IgG or IgM constant domains. Heavy chain and light chain constant domain amino acid sequences are known in the art. Non-limiting examples of Ig heavy chain yl constant region and Ig light chain λ and κ chains are provided for in Tables 8 and 6, respectively. 
     Still further, an antibody or antigen-binding portion thereof may be part of a larger immunoadhesion molecule, formed by covalent or noncovalent association of the antibody or antibody portion with one or more other proteins or peptides. Examples of such immunoadhesion molecules include use of the streptavidin core region to make a tetrameric scFv molecule (Kipriyanov, S. M., et al. (1995) Human Antibodies and Hybridomas 6:93-101) and use of a cysteine residue, a marker peptide and a C-terminal polyhistidine tag to make bivalent and biotinylated scFv molecules (Kipriyanov, S. M., et al. (1994) Mol. Immunol. 31:1047-1058). Antibody portions, such as Fab and F(ab′)2 fragments, can be prepared from whole antibodies using conventional techniques, such as papain or pepsin digestion, respectively, of whole antibodies. Moreover, antibodies, antibody portions and immunoadhesion molecules can be obtained using standard recombinant DNA techniques, as described herein. 
     An “isolated antibody”, as used herein, is intended to refer to an antibody that is substantially free of other antibodies having different antigenic specificities (e.g., an isolated antibody that specifically binds IgE is substantially free of antibodies that specifically bind antigens other than IgE). An isolated antibody that specifically binds, for example, human IgE may, however, have cross-reactivity to other antigens, such as IgE molecules from other species. Moreover, an isolated antibody may be substantially free of other cellular material and/or chemicals. 
     The term “CDR-grafted antibody” refers to antibodies which comprise heavy and light chain variable region sequences from one species but in which the sequences of one or more of the CDR regions of VH and/or VL are replaced with CDR sequences of another species, such as antibodies having human heavy and light chain variable regions in which one or more of the human CDRs (e.g., CDR3) has been replaced with murine CDR sequences. 
     The terms “Kabat numbering”, “Kabat definitions” and “Kabat labelling” are used interchangeably herein. These terms, which are recognized in the art, refer to a system of numbering amino acid residues which are more variable (i.e. hypervariable) than other amino acid residues in the heavy and light chain variable regions of an antibody, or an antigen binding portion thereof (Kabat et al. (1971)  Ann. NY Acad, Sci.  190:382-391 and, Kabat, E. A., et al. (1991)  Sequences of Proteins of Immunological Interest, Fifth Edition , U.S. Department of Health and Human Services, NIH Publication No. 91-3242). For the heavy chain variable region, the hypervariable region ranges from amino acid positions 31-35 (CDR-H1), residues 50-65 (CDR-H2) and residues 95-102 (CDR-H3) according to the Kabat numbering system. However, according to Chothia (Chothia et al., (1987) J. Mol. Biol., 196, 901-917 (1987)), the loop equivalent to CDR-H1 extends from residue 26 to residue 32. Thus, unless indicated otherwise “CDR-H1” as employed herein is intended to refer to residues 26 to 35, as described by a combination of the Kabat numbering system and Chothia&#39;s topological loop definition. For the light chain variable region, the hypervariable region ranges from amino acid positions 24 to 34 for CDRL1, amino acid positions 50 to 56 for CDRL2, and amino acid positions 89 to 97 for CDRL3. 
     As used herein, the terms “acceptor” and “acceptor antibody” refer to the antibody or nucleic acid sequence providing or encoding at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or 100% of the amino acid sequences of one or more of the framework regions. In some embodiments, the term “acceptor” refers to the antibody amino acid or nucleic acid sequence providing or encoding the constant region(s). In yet another embodiment, the term “acceptor” refers to the antibody amino acid or nucleic acid sequence providing or encoding one or more of the framework regions and the constant region(s). In a specific embodiment, the term “acceptor” refers to a human antibody amino acid or nucleic acid sequence that provides or encodes at least 80%, preferably, at least 85%, at least 90%, at least 95%, at least 98%, or 100% of the amino acid sequences of one or more of the framework regions. In accordance with this embodiment, an acceptor may contain at least 1, at least 2, at least 3, least 4, at least 5, or at least 10 amino acid residues that does (do) not occur at one or more specific positions of a human antibody. An acceptor framework region and/or acceptor constant region(s) may be, e.g., derived or obtained from a germline antibody gene, a mature antibody gene, a functional antibody (e.g., antibodies well-known in the art, antibodies in development, or antibodies commercially available). 
     As used herein, the term “CDR” refers to the complementarity determining region within antibody variable sequences. There are three CDRs in each of the variable regions of the heavy chain and the light chain, which are designated CDRH1, CDRH2 and CDRH3 for the heavy chain CDRs, and CDRL1, CDRL2, and CDRL3 for the light chain CDRs. The term “CDR set” as used herein refers to a group of three CDRs that occur in a single variable region capable of binding the antigen. The exact boundaries of these CDRs have been defined differently according to different systems. The system described by Kabat (Kabat et al., Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md. (1987) and (1991)) not only provides an unambiguous residue numbering system applicable to any variable region of an antibody, but also provides precise residue boundaries defining the three CDRs. These CDRs may be referred to as Kabat CDRs. Chothia and coworkers (Chothia &amp; Lesk, J. Mol. Biol. 196:901-917 (1987) and Chothia et al., Nature 342:877-883 (1989)) found that certain sub-portions within Kabat CDRs adopt nearly identical peptide backbone conformations, despite having great diversity at the level of amino acid sequence. These sub-portions were designated as L1, L2 and L3 or H1, H2 and H3 where the “L” and the “H” designates the light chain and the heavy chains regions, respectively. These regions may be referred to as Chothia CDRs, which have boundaries that overlap with Kabat CDRs. Other boundaries defining CDRs overlapping with the Kabat CDRs have been described by Padlan (FASEB J. 9:133-139 (1995)) and MacCallum (J Mol Biol 262(5):732-45 (1996)). Still other CDR boundary definitions may not strictly follow one of the above systems, but will nonetheless overlap with the Kabat CDRs, although they may be shortened or lengthened in light of prediction or experimental findings that particular residues or groups of residues or even entire CDRs do not significantly impact antigen binding. The methods used herein may utilize CDRs defined according to any of these systems, although preferred embodiments use Kabat or Chothia, or a mixture thereof, defined CDRs. 
     As used herein, the term “canonical” residue refers to a residue in a CDR or framework that defines a particular canonical CDR structure as defined by Chothia et al. (J. Mol. Biol. 196:901-907 (1987); Chothia et al., J. Mol. Biol. 227:799 (1992), both are incorporated herein by reference). According to Chothia et al., critical portions of the CDRs of many antibodies have nearly identical peptide backbone conformations despite great diversity at the level of amino acid sequence. Each canonical structure specifies primarily a set of peptide backbone torsion angles for a contiguous segment of amino acid residues forming a loop. 
     As used herein, the terms “donor” and “donor antibody” refer to an antibody providing one or more CDRs. In a preferred embodiment, the donor antibody is an antibody from a species different from the antibody from which the framework regions are obtained or derived. In the context of a humanized antibody, the term “donor antibody” refers to a non-human antibody providing one or more CDRs. 
     As used herein, the term “framework” or “framework sequence” refers to the remaining sequences of a variable region minus the CDRs. Because the exact definition of a CDR sequence can be determined by different systems, the meaning of a framework sequence is subject to correspondingly different interpretations. The six CDRs (CDR-L1, -L2, and -L3 of light chain and CDR-H1, -H2, and -H3 of heavy chain) also divide the framework regions on the light chain and the heavy chain into four sub-regions (FR1, FR2, FR3 and FR4) on each chain, in which CDR1 is positioned between FR1 and FR2, CDR2 between FR2 and FR3, and CDR3 between FR3 and FR4. Without specifying the particular sub-regions as FR1, FR2, FR3 or FR4, a framework region, as referred by others, represents the combined FR&#39;s within the variable region of a single, naturally occurring immunoglobulin chain. As used herein, a FR represents one of the four sub-regions, and FRs represents two or more of the four sub-regions constituting a framework region. 
     As used herein, the term “germline antibody gene” or “gene fragment” refers to an immunoglobulin sequence encoded by non-lymphoid cells that have not undergone the maturation process that leads to genetic rearrangement and mutation for expression of a particular immunoglobulin. See, e.g., Shapiro et al., Crit. Rev. Immunol. 22(3): 183-200 (2002); Marchalonis et al., Adv Exp Med Biol. 484:13-30 (2001). One of the advantages provided by various embodiments of the present invention takes advantage of the recognition that germline antibody genes are more likely than mature antibody genes to conserve essential amino acid sequence structures characteristic of individuals in the species, hence less likely to be recognized as from a foreign source when used therapeutically in that species. 
     As used herein, the term “key” residues refer to certain residues within the variable region that have more impact on the binding specificity and/or affinity of an antibody, in particular a humanized antibody. A key residue includes, but is not limited to, one or more of the following: a residue that is adjacent to a CDR, a potential glycosylation site (can be either N- or 0-glycosylation site), a rare residue, a residue capable of interacting with the antigen, a residue capable of interacting with a CDR, a canonical residue, a contact residue between heavy chain variable region and light chain variable region, a residue within the Vernier zone, and a residue in the region that overlaps between the Chothia definition of a variable heavy chain CDR1 and the Kabat definition of the first heavy chain framework. 
     The term “humanized antibody” generally refers to antibodies which comprise heavy and light chain variable region sequences from a non-human species (e.g., a rabbit, mouse, etc.) but in which at least a portion of the VH and/or VL sequence has been altered to be more “human-like”, i.e., more similar to human germline variable sequences. One type of humanized antibody is a CDR-grafted antibody, in which human CDR sequences are introduced into non-human VH and VL sequences to replace the corresponding nonhuman CDR sequences. Another type of humanized antibody is a CDR-grafted antibody, in which at least one non-human CDR is inserted into a human framework. The latter is typically the focus of the present invention. 
     In particular, the term “humanized antibody” as used herein, is an antibody or a variant, derivative, analog or fragment thereof which immuno-specifically binds to an antigen of interest and which comprises a framework (FR) region having substantially the amino acid sequence of a human antibody and a complementarity determining region (CDR) having substantially the amino acid sequence of a non-human antibody. As used herein, the term “substantially” in the context of a CDR refers to a CDR having an amino acid sequence at least 50, 55, 60, 65, 70, 75 or 80%, preferably at least 85%, at least 90%, at least 95%, at least 98% or at least 99% identical to the amino acid sequence of a non-human antibody CDR. In one embodiment, the humanized antibody has a CDR region having one or more (for example 1, 2, 3 or 4) amino acid substitutions, additions and/or deletions in comparison to the non-human antibody CDR. Further, the non-human CDR can be engineered to be more “human-like” or compatible with the human body, using known techniques. A humanized antibody comprises substantially all of at least one, and typically two, variable domains (Fab, Fab′, F(ab′)2, F(ab′)c, Fv) in which all or substantially all of the CDR regions correspond to those of a non-human immunoglobulin (i.e., donor antibody) and all or substantially all of the framework regions are those of a human immunoglobulin consensus sequence. Preferably, a humanized antibody also comprises at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin. In some embodiments, a humanized antibody contains both the light chain as well as at least the variable domain of a heavy chain. The antibody also may include the CH1, hinge, CH2, and CH3, or CH1, CH2, CH3, and CH4 of the heavy chain. In some embodiments, a humanized antibody only contains a humanized light chain. In some embodiments, a humanized antibody only contains a humanized heavy chain. In specific embodiments, a humanized antibody only contains a humanized variable domain of a light chain and/or humanized heavy chain. Though some of the mutations discussed herein may not be commonly “human”, these are insufficient for the anti-IgE antibody, or antigen binding agent, of the invention not to be “humanized”. 
     The humanized antibody can be selected from any class of immunoglobulins, including IgY, IgM, IgG, IgD, IgA and IgE, and any isotype, including without limitation IgA1, IgA2, IgG1, IgG2, IgG3 and IgG4. The humanized antibody may comprise sequences from more than one class or isotype, and particular constant domains may be selected to optimize desired effector functions using techniques well-known in the art. 
     The framework and CDR regions of a humanized antibody need not correspond precisely to the parental sequences, e.g., the donor antibody CDR or the consensus framework may be mutagenized by substitution, insertion and/or deletion of at least one amino acid residue so that the CDR or framework residue at that site does not correspond exactly to either the donor antibody or the consensus framework. In a preferred embodiment, such mutations, however, will not be extensive. Usually, at least 50, 55, 60, 65, 70, 75 or 80%, preferably at least 85%, more preferably at least 90%, and most preferably at least 95%, 98% or 99% of the humanized antibody residues will correspond to those of the parental FR and CDR sequences. In one embodiment, one or more (for example 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10) amino acid substitutions, additions and/or deletions may be present in the humanized antibody compared to the parental FR and CDR sequences (for instance compared to the omalizumab or omalizumab Fab sequences). As used herein, the term “consensus framework” refers to the framework region in the consensus immunoglobulin sequence. As used herein, the term “consensus immunoglobulin sequence” refers to the sequence formed from the most frequently occurring amino acids (or nucleotides) in a family of related immunoglobulin sequences (See e.g., Winnaker, From Genes to Clones (Verlagsgesellschaft, Weinheim, Germany 1987). In a family of immunoglobulins, each position in the consensus sequence is occupied by the amino acid occurring most frequently at that position in the family. If two amino acids occur equally frequently, either can be included in the consensus sequence. 
     As used herein, “Vernier” zone refers to a subset of framework residues that may adjust CDR structure and fine-tune the fit to antigen as described by Foote and Winter (1992, J. Mol. Biol. 224:487-499, which is incorporated herein by reference). Vernier zone residues form a layer underlying the CDRs and may impact on the structure of CDRs and the affinity of the antibody. 
     As used herein, the term “neutralizing” refers to neutralization of biological activity of IgE, when an anti-IgE antibody, or antigen binding agent, of the invention described herein specifically binds the IgE protein. Neutralizing may be the result of different ways of binding of said antibody to IgE. Preferably a neutralizing antibody is an antibody whose binding to IgE results in neutralization of a biological activity of IgE. Preferably the neutralizing binding protein binds IgE and decreases a biologically activity of IgE by at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 50%, 60%, 80%, 85%, or more. Neutralization of a biological activity of IgE by a neutralizing antibody can be assessed by measuring one or more indicators of IgE biological activity described herein. 
     A “neutralizing monoclonal antibody” as used herein is intended to refer to a preparation of antibody molecules, which upon binding to IgE are able to inhibit or reduce the biological activity of IgE either partially or fully. 
     As used herein, the term “attenuation,” “attenuate,” and the like refers to the lessening or reduction in the severity of a symptom or condition caused by elevated serum IgE levels. 
     The term “epitope” or “antigenic determinant” includes any polypeptide determinant capable of specific binding to an immunoglobulin or T-cell receptor. In certain embodiments, epitope determinants include chemically active surface groupings of molecules such as amino acids, sugar side chains, phosphoryl, or sulfonyl, and, in certain embodiments, may have specific three dimensional structural characteristics, and/or specific charge characteristics. An epitope is a region of an antigen that is bound by an antibody. In certain embodiments, an antibody is said to specifically bind an antigen when it preferentially recognizes its target antigen in a complex mixture of proteins and/or macromolecules. 
     The term “k on ”, as used herein, is intended to refer to the on rate constant for association of an antibody to the antigen to form the antibody/antigen complex as is known in the art. 
     The term “k off ”, as used herein, is intended to refer to the off rate constant for dissociation of an antibody from the antibody/antigen complex as is known in the art. 
     The term “k d ” or “k D ”, as used herein, is intended to refer to the dissociation constant of a particular antibody-antigen interaction as is known in the art. 
     The strength, or affinity of immunological binding interactions can be expressed in terms of the dissociation constant (k D  or k d ) of the interaction, wherein a smaller k d  represents a greater or higher affinity. Immunological binding properties of selected polypeptides can be quantified using methods well known in the art. One such method involves measuring the rates of antigen-binding site/antigen complex formation and dissociation, wherein those rates depend on the concentrations of the complex partners, the affinity of the interaction, and geometric parameters that equally influence the rate in both directions. Thus, both the “on rate constant” (“k on ”) and the “off rate constant” (“k off ”) can be determined by calculation of the concentrations and the actual rates of association and dissociation. (Nature 361:186-87 (1993)). The ratio of k off /k on  enables the cancellation of all parameters not related to affinity, and is equal to the dissociation constant k d . Davies et al. (1990) Annual Rev Biochem 59:439-473. 
     The term “antibody conjugate” refers to a binding protein, such as an antibody or antibody fragment or binding portion thereof, chemically linked to a second chemical moiety, such as a therapeutic or cytotoxic agent. The term “agent” is used herein to denote a chemical compound, a mixture of chemical compounds, a biological macromolecule, or an extract made from biological materials. 
     The terms “crystal”, and “crystallized” as used herein, refer to an antibody, or antigen binding portion thereof, that exists in the form of a crystal. Crystals are one form of the solid state of matter, which is distinct from other forms such as the amorphous solid state or the liquid crystalline state. Crystals are composed of regular, repeating, three-dimensional arrays of atoms, ions, molecules (e.g., proteins such as antibodies), or molecular assemblies (e.g., antigen/antibody complexes). These three-dimensional arrays are arranged according to specific mathematical relationships that are well-understood in the field. The fundamental unit, or building block, that is repeated in a crystal is called the asymmetric unit. Repetition of the asymmetric unit in an arrangement that conforms to a given, well-defined crystallographic symmetry provides the “unit cell” of the crystal. Repetition of the unit cell by regular translations in all three dimensions provides the crystal. See Giege, R. and Ducruix, A. Barrett, Crystallization of Nucleic Acids and Proteins, a Practical Approach, 2nd ea., pp. 20 1-16, Oxford University Press, New York, N.Y., (1999).” 
     The term “polynucleotide” as referred to herein means a polymeric form of two or more nucleotides, either ribonucleotides or deoxynucleotides or a modified form of either type of nucleotide. The term includes single and double stranded forms of DNA but preferably is double-stranded DNA. 
     The term “isolated polynucleotide” as used herein means a polynucleotide (e.g., of genomic, cDNA, or synthetic origin, or some combination thereof) that, by virtue of its origin, the “isolated polynucleotide” is not associated with all or a portion of a polynucleotide with which the “isolated polynucleotide” is found in nature; is operably linked to a polynucleotide that it is not linked to in nature; or does not occur in nature as part of a larger sequence. 
     The term “vector,” as used herein, is intended to refer to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a “plasmid,” which refers to a circular double stranded DNA loop into which additional DNA segments may be ligated. Another type of vector is a viral vector, wherein additional DNA segments may be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as “recombinant expression vectors” (or simply, “expression vectors”). In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, “plasmid” and “vector” may be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions. 
     The term “operably linked” refers to a juxtaposition wherein the components described are in a relationship permitting them to function in their intended manner. A control sequence “operably linked” to a coding sequence is ligated in such a way that expression of the coding sequence is achieved under conditions compatible with the control sequences. “Operably linked” sequences include both expression control sequences that are contiguous with the gene of interest and expression control sequences that act in trans or at a distance to control the gene of interest. The term “expression control sequence” as used herein refers to polynucleotide sequences, which are necessary to effect the expression and processing of coding sequences to which they are ligated. Expression control sequences include appropriate transcription initiation, termination, promoter and enhancer sequences; efficient RNA processing signals such as splicing and polyadenylation signals; sequences that stabilize cytoplasmic mRNA; sequences that enhance translation efficiency (i.e., Kozak consensus sequence); sequences that enhance protein stability; and when desired, sequences that enhance protein secretion. The nature of such control sequences differs depending upon the host organism; in prokaryotes, such control sequences generally include promoter, ribosomal binding site, and transcription termination sequence; in eukaryotes, generally, such control sequences include promoters and transcription termination sequence. The term “control sequences” is intended to include components whose presence is essential for expression and processing, and can also include additional components whose presence is advantageous, for example, leader sequences and fusion partner sequences. 
     “Transformation,” as defined herein, refers to any process by which exogenous DNA enters a host cell. Transformation may occur under natural or artificial conditions using various methods well known in the art. Transformation may rely on any known method for the insertion of foreign nucleic acid sequences into a prokaryotic or eukaryotic host cell. The method is selected based on the host cell being transformed and may include, but is not limited to, viral infection, electroporation, lipofection, and particle bombardment. Such “transformed” cells include stably transformed cells in which the inserted DNA is capable of replication either as an autonomously replicating plasmid or as part of the host chromosome. They also include cells which transiently express the inserted DNA or RNA for limited periods of time. 
     The term “recombinant host cell” (or simply “host cell”), as used herein, is intended to refer to a cell into which exogenous DNA has been introduced. It should be understood that such terms are intended to refer not only to the particular subject cell, but, to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term “host cell” as used herein. Preferably host cells include prokaryotic and eukaryotic cells selected from any of the Kingdoms of life. Preferred eukaryotic cells include protist, fungal, plant and animal cells. Most preferably host cells include but are not limited to the prokaryotic cell line  E. coli ; mammalian cell lines CHO, HEK 293 and COS; the insect cell line Sf9; and the fungal cell  Saccharomyces cerevisiae.    
     Standard techniques may be used for recombinant DNA, oligonucleotide synthesis, and tissue culture and transformation (e.g., electroporation, lipofection). Enzymatic reactions and purification techniques may be performed according to manufacturer&#39;s specifications or as commonly accomplished in the art or as described herein. The foregoing techniques and procedures may be generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification. See e.g., Sambrook et al. Molecular Cloning: A Laboratory Manual (2d ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)), which is incorporated herein by reference for any purpose. 
     As used herein, the term “effective amount” refers to the amount of a therapy which is sufficient to reduce or ameliorate the severity and/or duration of a disorder or one or more symptoms thereof, prevent the advancement of a disorder, cause regression of a disorder, prevent the recurrence, development, onset or progression of one or more symptoms associated with a disorder, detect a disorder, or enhance or improve the prophylactic or therapeutic effect(s) of another therapy (e.g. prophylactic or therapeutic agent). 
     The specific region or epitope mapping of human IgE protein provided herein can be identified by any suitable epitope mapping method known in the art in combination with any one of the antibodies provided by the present invention. Examples of such methods include screening peptides of varying lengths derived from IgE for binding to the antibody of the present invention with the smallest fragment that can specifically bind to the antibody containing the sequence of the epitope recognized by the antibody. The IgE peptides may be produced synthetically or by proteolytic digestion of the IgE protein. Peptides that bind the antibody can be identified by, for example, mass spectrometric analysis. In another example, NMR spectroscopy or X-ray crystallography can be used to identify the epitope bound by an antibody of the present invention. Crystallization and X-ray crystallography techniques are preferred for determining the structure of IgE and the epitope on IgE that the anti-IgE antibody, or antigen binding agent, of the invention binds to. 
     Antibodies for use in the invention may be generated using single lymphocyte antibody methods by cloning and expressing immunoglobulin variable region cDNAs generated from single lymphocytes selected for the production of specific antibodies by, for example, the methods described by Babcook, J. et al., 1996, Proc. Natl. Acad. Sci. USA 93(15):7843-78481; WO92/02551; WO2004/051268 and International Patent Application number WO2004/106377. Screening for antibodies can be performed using assays to measure binding to human IgE and/or assays to measure the ability to block IgE binding to its natural receptor. An example of a binding assay is an ELISA. 
     Humanized antibodies (which include CDR-grafted antibodies) are antibody molecules having one or more complementarity determining regions (CDRs) from a non-human species (e.g., a rabbit or mouse) and a framework region from a human immunoglobulin molecule (see, e.g. U.S. Pat. No. 5,585,089; WO91/09967). It will be appreciated that it may only be necessary to transfer the specificity determining residues of the CDRs rather than the entire CDR (see for example, Kashmiri et al., 2005, Methods, 36, 25-34). Humanized antibodies may optionally further comprise one or more framework residues derived from the non-human species from which the CDRs were derived. The latter are often referred to as donor residues. The antibody molecules of the present invention suitably have a binding affinity (K D ) of less than 2 nM. Affinity may be measured using any suitable method known in the art, including BIAcore, as described in the Examples herein (see Example 6), using isolated natural or recombinant IgE or a suitable fusion protein/polypeptide. 
     The affinity of an antibody or antigen binding agent of the present invention, as well as the extent to which a binding agent (such as an antibody) inhibits binding, can be determined by one of ordinary skill in the art using conventional techniques, for example those described by Scatchard et al. (Ann. KY. Acad. Sci. 51:660-672 (1949)) or by surface plasmon resonance (SPR) using systems such as BIAcore. For surface plasmon resonance, target molecules are immobilized on a solid phase and exposed to ligands in a mobile phase running along a flow cell. If ligand binding to the immobilized target occurs, the local refractive index changes, leading to a change in SPR angle, which can be monitored in real time by detecting changes in the intensity of the reflected light. The rates of change of the SPR signal can be analysed to yield apparent rate constants for the association and dissociation phases of the binding reaction. The ratio of these values gives the apparent equilibrium constant (affinity) (see, e.g., Wolff et al, Cancer Res. 53:2560-65 (1993)). 
     It will be appreciated that the affinity of antibodies provided by the present invention may be altered using any suitable method known in the art. The present invention therefore also relates to variants of the antibody molecules of the present invention, which have an improved affinity for IgE. Such variants can be obtained by a number of affinity maturation protocols including mutating the CDRs (Yang et al., J. Mol. Biol., 254, 392-403, 1995), chain shuffling (Marks et al., Bio/Technology, 10, 779-783, 1992), use of mutator strains of  E. coli  (Low et al., J. Mol. Biol., 250, 359-368, 1996), DNA shuffling (Patten et al., Curr. Opin. Biotechnol., 8, 724-733, 1997), phage display (Thompson et al., J. Mol. Biol., 256, 77-88, 1996) and sexual PCR (Crameri et al., Nature, 391, 288-291, 1998). Vaughan et al. (supra) discusses these methods of affinity maturation. 
     Humanized Antibodies and Antigen Binding Agents 
     In one aspect of the present invention, provided herein are humanized anti-IgE monoclonal antibodies and antigen binding agents. Humanized antibodies are antibodies wherein the heavy and/or light chain contains one or more CDRs (including, if desired, one or more modified CDRs) from a donor antibody (e.g. a non-human antibody such as a murine or rabbit monoclonal antibody) grafted into a heavy and/or light chain variable region framework of an acceptor antibody (e.g. a human antibody). For a review, see Vaughan et al, Nature Biotechnology, 16, 535-539, 1998. 
     In one embodiment, rather than the entire CDR being transferred, only one or more of the specificity determining residues from any one of the CDRs described herein above are transferred to the human antibody framework (see for example, Kashmiri et al., 2005, Methods, 36, 25-34). In one embodiment only the specificity determining residues from one or more of the CDRs described herein are transferred to the human antibody framework. In another embodiment only the specificity determining residues from each of the CDRs described herein are transferred to the human antibody framework. 
     When the CDRs or specificity determining residues are grafted, any appropriate acceptor variable region framework sequence may be used having regard to the class/type of the donor antibody from which the CDRs are derived, including mouse, rabbit, primate and human framework regions. 
     Suitably, the humanized antibody according to the present invention has a variable domain comprising human acceptor framework regions as well as one or more of the CDRs provided specifically herein. Thus, provided in one embodiment is a humanized monoclonal antibody which binds human IgE wherein the variable domain comprises human acceptor framework regions (with optional mutations as described herein) and non-human donor CDRs. 
     Construction of CDR-grafted antibodies is generally described in European Patent Application EP-A-0239400, which discloses a process in which the CDRs of a mouse monoclonal antibody are grafted onto the framework regions of the variable domains of a human immunoglobulin by site directed mutagenesis using long oligonucleotides, and is incorporated herein. The CDRs determine the antigen binding specificity of antibodies and are relatively short peptide sequences carried on the framework regions of the variable domains. 
     The earliest work on humanizing monoclonal antibodies by CDR-grafting was carried out on monoclonal antibodies recognizing synthetic antigens, such as NP. However, examples in which a mouse monoclonal antibody recognizing lysozyme and a rat monoclonal antibody recognizing an antigen on human T-cells were humanized by CDR-grafting have been described by Verhoeyen et al. (Science, 239, 1534-1536, 1988) and Riechmann et al (Nature, 332, 323-324, 1988), respectively. Antibody humanization is achieved by grafting CDRs of a non-human antibody, such as a mouse, rat, goat, or rabbit antibody, onto a “similar” human framework (acceptor) and selecting minimal number of key framework residues (back-mutations) that are manually selected from the donor monoclonal antibody and incorporated into human acceptor framework in order to maintain the original CDR conformation. Such methods are known in the art, and include those described in Jones et al., Nature 321:522 (1986); Verhoeyen et al., Science 239:1534 (1988)), Sims et al., J. Immunol. 151: 2296 (1993); Chothia and Lesk, J. Mol. Biol. 196:901 (1987), Carter et al., Proc. Natl. Acad. Sci. U.S.A. 89:4285 (1992); Presta et al., J. Immunol. 151:2623 (1993), Padlan, Molecular Immunology 28(4/5):489-498 (1991); Studnicka et al., Protein Engineering 7(6):805-814 (1994); Roguska. et al., PNAS 91:969-973 (1994); PCT publication WO 91/09967, PCT: US98/16280, US96/18978, US91/09630, US91/05939, US94/01234, GB89/01334, GB91/01134, GB92/01755; WO90/14443, WO90/14424, WO90/14430, EP 229246, EP 592,106; EP 519,596, EP 239,400, U.S. Pat. Nos. 5,565,332, 5,723,323, 5,976,862, 5,824,514, 5,817,483, 5,814,476, 5,763,192, 5,723,323, 5,766,886, 5,714,352, 6,204,023, 6,180,370, 5,693,762, 5,530,101, 5,585,089, 5,225,539; 4,816,567, which are incorporated herein. 
     The human variable heavy and light chain germline subfamily classification can be derived from the Kabat germline subgroup designations: VH1, VH2, VH3, VH4, VHS, VH6 or VH7 for a particular VH sequence and JH1, JH2, JH3, JH4, JH5, and JH6 for a for a particular variable heavy joining group for framework 4; VK1, VK2, VK3, VK4, VK5 or VK6 for a particular VL kappa sequence for framework 1, 2, and 3, and JK1, JK2, JK3, JK4, or JK5 for a particular kappa joining group for framework 4; or VL1, VL2, VL3, VL4, VLS, VL6, VL7, VL8, VL9, or VL10 for a particular VL lambda sequence for framework 1, 2, and 3, and JL1, JL2, JL3, or JL7 for a particular lambda joining group for framework 4. 
     The constant region domains of the antibody molecule of the present invention, if present, may be selected having regard to the proposed function of the antibody molecule, and in particular the effector functions which may be required. For example, the constant region domains may be human IgA, IgD, IgE, IgG or IgM domains. In particular embodiments, human IgG constant region domains may be used, especially of the IgG1 and IgG3 isotypes when the antibody molecule is intended for therapeutic uses and antibody effector functions are required. Alternatively, IgG2 and IgG4 isotypes may be used when the antibody molecule is intended for therapeutic purposes and antibody effector functions are not required. It will be appreciated that sequence variants of these constant region domains may also be used. For example IgG4 molecules in which the serine at position 241 has been changed to proline as described in Angal et al., Molecular Immunology, 1993, 30 (1), 105-108 may be used. It will also be understood by one skilled in the art that antibodies may undergo a variety of posttranslational modifications. The type and extent of these modifications often depends on the host cell line used to express the antibody as well as the culture conditions. Such modifications may include variations in glycosylation, methionine oxidation, diketopiperazine formation, aspartate isomerization and asparagine deamidation. A frequent modification is the loss of a carboxy-terminal basic residue (such as lysine or arginine) due to the action of carboxypeptidases (as described in Harris, RJ. Journal of Chromatography 705:129-134, 1995). Accordingly, the C-terminal lysine of the antibody heavy chain may be absent. 
     CDR and Human Framework Modifications 
     Riechmann et al., found that the transfer of the CDRs alone (as defined by Kabat (Kabat et al. (supra) and Wu et al., J. Exp. Med., 132, 211-250, 1970)) was not sufficient to provide satisfactory antigen binding activity in the CDR-grafted product. It was found that a number of framework residues have to be altered so that they correspond to those of the donor framework region. Proposed criteria for selecting which framework residues need to be altered are described in International Patent Application WO 90/07861, which is incorporated herein. 
     The substitution of non-human CDRs into a human variable domain framework is most likely to result in retention of the CDR&#39;s correct spatial orientation if the human variable domain framework adopts the same or similar conformation to the non-human variable framework from which the CDRs originated. This is achieved by obtaining the human variable domains from human antibodies whose framework sequences exhibit a high degree of sequence identity with the non-human variable framework domains from which the CDRs were derived. As described above, the heavy and light chain variable framework regions can be derived from the same or different human antibody sequences. The human antibody sequences can be the sequences of naturally occurring human antibodies or can be consensus sequences of several human antibodies. See Kettleborough et al, Protein Engineering 4:773 (1991); Kolbinger et al., Protein Engineering 6:971 (1993) and Carter et al, WO 92/22653. 
     Having identified the complementarity determining regions of the non-human donor immunoglobulin and appropriate human acceptor immunoglobulins, the next step is to determine which, if any, residues from these components should be substituted to optimize the properties of the resulting humanized antibody. In general, substitution of human amino acid residues with non-human amino acid residues should be minimized, because introduction of non-human residues increases the risk of the antibody eliciting a human-anti-donor-antibody (HADA) response in humans. Art-recognized methods of determining immune response can be performed to monitor a HADA response in a particular host or during clinical trials. Hosts administered humanized antibodies can be given an immunogenicity assessment at the beginning and throughout the administration of said therapy. The HADA response is measured, for example, by detecting antibodies to the humanized therapeutic reagent, in serum samples from the host using a method known to one in the art, including surface plasmon resonance technology (BIACORE) and/or solid-phase ELISA analysis. 
     The selection of amino acid residues for substitution (also “mutation” herein) is determined, in part, by computer modelling. Computer hardware and software are described herein for producing three-dimensional images of immunoglobulin molecules. In general, molecular models are produced starting from solved structures for immunoglobulin chains or domains thereof. The chains to be modelled are compared for amino acid sequence similarity with chains or domains of solved three-dimensional structures, and the chains or domains showing the greatest sequence similarity is/are selected as starting points for construction of the molecular model. Chains or domains sharing at least 50% sequence identity are selected for modelling, and preferably those sharing at least 60%, 70%, 80%, 90%, sequence identity or more are selected for modelling. The solved starting structures are modified to allow for differences between the actual amino acids in the immunoglobulin chains or domains being modelled, and those in the starting structure. The modified structures are then assembled into a composite immunoglobulin. Finally, the model is refined by energy minimization and by verifying that all atoms are within appropriate distances from one another and that bond lengths and angles are within chemically acceptable limits. 
     The selection of amino acid residues for substitution can also be determined, in part, by examination of the characteristics of the amino acids at particular locations, or empirical observation of the effects of substitution or mutagenesis of particular amino acids. For example, when an amino acid differs between a donor variable region framework residue and a selected human variable region framework residue, the human framework amino acid should usually be substituted by the equivalent framework amino acid from the donor antibody when it is reasonably expected that the amino acid:
         (1) noncovalently binds antigen directly,   (2) is adjacent to a CDR region,   (3) otherwise interacts with a CDR region (e.g., is within about 3-6 Angstrom of a CDR region as determined by computer modelling), or   (4) participates in the VL-VH interface.       

     Residues which “noncovalently bind antigen directly” include amino acids in positions in framework regions which have a good probability of directly’ interacting with amino acids on the antigen according to established chemical forces, for example, by hydrogen bonding, Van der Waals forces, hydrophobic interactions, and the like. CDR and framework regions are as defined by Kabat et al. or Chothia et al, supra. When framework residues, as defined by Kabat et al, supra, constitute structural loop residues as defined by Chothia et al, supra, the amino acids present in the donor antibody may be selected for substitution into the humanized antibody. Residues which are “adjacent to a CDR region” include amino acid residues in positions immediately adjacent to one or more of the CDRs in the primary sequence of the humanized immunoglobulin chain, for example, in positions immediately adjacent to a CDR as defined by Kabat, or a CDR as defined by Chothia (See e.g., Chothia and Lesk 1 MB 196:901 (1987)). These amino acids are particularly likely to interact with the amino acids in the CDRs and, if chosen from the acceptor, to distort the donor CDRs and reduce affinity. Moreover, the adjacent amino acids may interact directly with the antigen (Amit et al, Science, 233:747 (1986), which is incorporated herein by reference) and selecting these amino acids from the donor may be desirable to keep all the antigen contacts that provide affinity in the original antibody. As described herein, FR sequences may also be substituted/mutated to improve the affinity of the anti-IgE antibody, or antigen binding agent, of the invention for IgE (and/or to extend its interaction or epitope on Cε2 of IgE). 
     Residues that “otherwise interact with a CDR region” include those that are determined by secondary structural analysis to be in a spatial orientation sufficient to effect a CDR region. In one embodiment, residues that “otherwise interact with a CDR region” are identified by analysing a three-dimensional model of the donor immunoglobulin (e.g., a computer-generated model). A three-dimensional model, typically of the original donor antibody, shows that certain amino acids outside of the CDRs are close to the CDRs and have a good probability of interacting with amino acids in the CDRs by hydrogen bonding, Van der Waals forces, hydrophobic interactions, etc. At those amino acid positions, the donor immunoglobulin amino acid rather than the acceptor immunoglobulin amino acid may be selected. Amino acids according to this criterion will generally have a side chain atom within about 3 angstrom units (A) of some atom in the CDRs and must contain an atom that could interact with the CDR atoms according to established chemical forces, such as those listed above. In the case of atoms that may form a hydrogen bond, the 3 A° is measured between their nuclei, but for atoms that do not form a bond, the 3 A° is measured between their Van der Waals surfaces. Hence, in the latter case, the nuclei must be within about 6 A° (3 A plus the sum of the Van der Waals radii) for the atoms to be considered capable of interacting. In many cases the nuclei will be from 4 or 5 to 6 A° apart. In determining whether an amino acid can interact with the CDRs, it is preferred not to consider the last 8 amino acids of heavy chain CDR 2 as part of the CDRs, because from the viewpoint of structure, these 8 amino acids behave more as part of the framework. 
     Amino acids that are capable of interacting with amino acids in the CDRs (or FRs), may be identified in yet another way. The solvent accessible surface area of each framework amino acid is calculated in two ways: (1) in the intact antibody, and (2) in a hypothetical molecule consisting of the antibody with its CDRs removed. A significant difference between these numbers of about 10 square angstroms or more shows that access of the framework amino acid to solvent is at least partly blocked by the CDRs, and therefore that the amino acid is making contact with the CDRs. Solvent accessible surface area of an amino acid may be calculated based on a three-dimensional model of an antibody, using algorithms known in the art (e.g., Connolly, J. Appl. Cryst. 16:548 (1983) and Lee and Richards, J. Mol. Biol. 55:379 (1971), both of which are incorporated herein by reference). Framework amino acids may also occasionally interact with the CDRs indirectly, by affecting the conformation of another framework amino acid that in turn contacts the CDRs. 
     Particular amino acids at several positions in the framework are known to be capable of interacting with the CDRs in many antibodies (Chothia and Lesk, supra, Chothia et al, supra and Tramontano et al, J. Mol. Biol. 215:175 (1990), all of which are incorporated herein by reference). Notably, the amino acids at positions 2, 48, 64, and 71 of the light chain and 71 and 94 of the heavy chain (numbering according to Kabat) are known to be capable of interacting with the CDRs in many antibodies. The amino acids at positions 35 in the light chain and 93 and 103 in the heavy chain are also likely to interact with the CDRs. At all these numbered positions, choice of the donor amino acid rather than the acceptor amino acid (when they differ) to be in the humanized immunoglobulin is preferred. On the other hand, certain residues capable of interacting with the CDR region, such as the first 5 amino acids of the light chain, may sometimes be chosen from the acceptor immunoglobulin without loss of affinity in the humanized immunoglobulin. 
     Residues which “participate in the VL-VH interface” or “packing residues” include those residues at the interface between VL and VH as defined, for example, by Novotny and Haber, Proc. Natl. Acad. Sci. USA, 82:4592-66 (1985) or Chothia et al, supra. Generally, unusual packing residues should be retained in the humanized antibody if they differ from those in the human frameworks. 
     In general, one or more of the amino acids fulfilling the above criteria is substituted. In some embodiments, all or most of the amino acids fulfilling the above criteria are substituted. Occasionally, there is some ambiguity about whether a particular amino acid meets the above criteria, and alternative variant immunoglobulins are produced, one of which has that particular substitution, the other of which does not. Alternative variant immunoglobulins so produced can be tested in any of the assays described herein for the desired activity, and the preferred immunoglobulin selected. 
     Usually the CDR regions in humanized antibodies are substantially identical, and more usually, identical to the corresponding CDR regions of the donor antibody. Although not usually desirable, it is sometimes possible to make one or more conservative amino acid substitutions of CDR residues without appreciably affecting the binding affinity of the resulting humanized immunoglobulin. By conservative or similar substitutions is intended combinations such as, for example, leucine being substituted for isoleucine or valine. Other amino acids which can often be substituted for one another include but are not limited to: 
     phenylalanine, tyrosine and tryptophan (amino acids having aromatic side chains); 
     lysine, arginine and histidine (amino acids having basic side chains); 
     aspartate and glutamate (amino acids having acidic side chains); 
     asparagine and glutamine (amino acids having amide side chains); and, 
     cysteine and methionine (amino acids having sulphur-containing side chains). 
     Additional candidates for substitution are acceptor human framework amino acids that are unusual or “rare” for a human immunoglobulin at that position. These amino acids can be substituted with amino acids from the equivalent position of the donor antibody or from the equivalent positions of more typical human immunoglobulins. For example, substitution may be desirable when the amino acid in a human framework region of the acceptor immunoglobulin is rare for that position and the corresponding amino acid in the donor immunoglobulin is common for that position in human immunoglobulin sequences; or when the amino acid in the acceptor immunoglobulin is rare for that position and the corresponding amino acid in the donor immunoglobulin is also rare, relative to other human sequences. These criteria help ensure that an atypical amino acid in the human framework does not disrupt the antibody structure. Moreover, by replacing an unusual human acceptor amino acid with an amino acid from the donor antibody that happens to be typical for human antibodies, the humanized antibody may be made less immunogenic. 
     The term “rare”, as used herein, indicates an amino acid occurring at that position in less than about 20% but usually less than about 10% of sequences in a representative sample of sequences, and the term “common,” as used herein, indicates an amino acid occurring in more than about 25% but usually more than about 50% of sequences in a representative sample. For example, all human light and heavy chain variable region sequences are respectively grouped into “subgroups” of sequences that are especially homologous to each other and have the same amino acids at certain critical positions (Kabat et al, supra). When deciding whether an amino acid in a human acceptor sequence is “rare” or “common” among human sequences, it will often be preferable to consider only those human sequences in the same subgroup as the acceptor sequence. 
     Additional candidates for substitution are acceptor framework residues that correspond to a rare or unusual donor framework residue. Rare or unusual donor framework residues are those that are rare or unusual (as defined herein) for donor antibodies at that position. For donor antibodies, the subgroup can be determined according to Kabat and residue positions identified which differ from the consensus. These donor specific differences may point to somatic mutations in the donor sequence, which enhance activity. Unusual residues that are predicted to affect binding are retained, whereas residues predicted to be unimportant for binding could be substituted. 
     Additional candidates for substitution are non-germline residues occurring in an acceptor framework region. For example, when an acceptor antibody chain (i.e., a human antibody chain sharing significant sequence identity with the donor antibody chain) is aligned to a germline antibody chain (likewise sharing significant sequence identity with the donor chain), residues not matching between acceptor chain framework and the germline chain framework can be substituted with corresponding residues from the germline sequence. 
     Other than the specific amino acid substitutions discussed above, the framework regions of humanized immunoglobulins are usually substantially identical, and more usually, identical to the framework regions of the human antibodies from which they were derived (except as described herein for the purposes of the present invention). Of course, many of the amino acids in the framework region make little or no direct contribution to the specificity or affinity of an antibody. Thus, many individual conservative substitutions of framework residues can be tolerated without appreciable change of the specificity or affinity of the resulting humanized immunoglobulin. Thus, in one embodiment the variable framework region of the humanized immunoglobulin shares at least 65, 75 or 85% sequence similarity or identity to a human variable framework region sequence or consensus of such sequences. In another embodiment, the variable framework region of the humanized immunoglobulin shares at least 90%, preferably 95%, more preferably 96%, 97%, 98%, or 99%, sequence similarity or identity to a human variable framework region sequence or consensus of such sequences. In general, however, such substitutions are undesirable (except those described herein). 
     As used herein, degrees of identity and similarity can be readily calculated, for example as described in Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing. Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part 1, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987, Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991, the BLAST™ software available from NCBI (Altschul, S. F. et al., 1990, J. Mol. Biol. 215:403-410; Gish, W. &amp; States, D. J. 1993, Nature Genet. 3:266-272. Madden, T. L. et al., 1996, Meth. Enzymol. 266:131-141; Altschul, S. F. et al., 1997, Nucleic Acids Res. 25:3389-3402; Zhang, J. &amp; Madden, T. L. 1997, Genome Res. 7:649-656, which are incorporated by reference herein. 
     A number of reviews discussing CDR-grafted antibodies have been published, including Vaughan et al. (Nature Biotechnology, 16, 535-539, 1998), which is incorporated by reference herein. 
     The anti-IgE antibodies of the present invention may include further additional binding domains for example as per the molecule DVD-Ig as disclosed in WO 2007/024715, or the so-called (FabFv)2Fc described in WO2011/030107. Thus, antibody as employed herein includes bi, tri or tetra-valent full length antibodies. 
     Antigen Binding Agents 
     Antigen binding agents include single chain antibodies (i.e. a full length heavy chain and light chain); Fab, modified Fab, Fab′, modified Fab′, F(ab′)2, Fv, Fab-Fv, Fab-dsFv, single domain antibodies (e.g. VII or VL or VHH) for example as described in WO 2001090190, scFv, bi, tri or tetra-valent antibodies, Bis-scFv, diabodies, tribodies, triabodies, tetrabodies and epitope-antigen binding agents of any of the above (see for example Holliger and Hudson, 2005, Nature Biotech. 23(9):1126-1136; Adair and Lawson, 2005, Drug Design Reviews—Online 2(3), 209-217). The methods for creating and manufacturing these antibody fragments are well known in the art (see for example Verma et al., 1998, Journal of Immunological Methods, 216, 165-181). The Fab-Fv format was first disclosed in WO2009/040562 and the disulphide stabilised versions thereof, the Fab-dsFv was first disclosed in WO2010/035012. Other antibody fragments for use in the present invention include the Fab and Fab′ fragments described in International patent applications WO2005/003169, WO2005/003170, and WO2005/003171. Multi-valent antibodies may comprise multiple specificities e.g. bispecific or may be monospecific (see for example WO 92/22583 and WO05/113605). One such example of the latter is a Tri-Fab (or TFM) as described in WO92/22583. 
     A typical Fab′ molecule comprises a heavy and a light chain pair in which the heavy chain comprises a variable region VH, a constant domain CH1 and a natural or modified hinge region and the light chain comprises a variable region VL and a constant domain CL. 
     In one embodiment, there is provided a dimer of a Fab′ according to the present disclosure to create a F(ab′)2 for example dimerization may be through a natural hinge sequence described herein, or derivative thereof, or a synthetic hinge sequence. 
     An antibody binding domain will generally comprise 6 CDRs, three from a heavy chain and three from a light chain. In one embodiment, the CDRs are in a framework and together form a variable region. Thus in one embodiment, the antigen binding agent includes a binding domain specific for IgE comprising a light chain variable region and a heavy chain variable region. 
     It will be appreciated that one or more (for example 1, 2, 3 or 4) amino acid substitutions, additions and/or deletions may be made to the CDRs or other sequences (e.g variable domains) provided by the present invention, as described above or below, without significantly altering the ability of the antibody to bind to IgE. The effect of any amino acid substitutions, additions and/or deletions can be readily tested by one skilled in the art, for example by using the methods described herein, in particular in the Examples. 
     In one embodiment, one or more (for example 1, 2, 3 or 4) amino acid substitutions, additions and/or deletions may be made to the CDRs or framework region employed in the antibody or fragment provided by the present invention so that the binding affinity (K D ) of the anti-IgE antibody, or antigen binding agent, of the invention to IgE is less than 2, 1.9, 1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, or 0.3 nM. In one embodiment, provided is a modified humanized antibody wherein modifications have been made to either the CDRs, framework regions, or both, in order to decrease the K D  for example to less than 2, 1.9, 1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, or 0.3 nM. 
     The antibody fragment of the present invention includes Fab, Fab′, F(ab′)2, scFv, diabody, scFAb, dFv, single domain light chain antibodies, dsFv, a peptide comprising CDR, and the like. 
     A Fab is an antibody fragment having a molecular weight of about 50,000 and antigen binding activity, in which about a half of the N-terminal side of H chain and the entire L chain, among fragments obtained by treating IgG with a protease, papain (cut at an amino acid residue at position 224 of the H chain), are bound together through a disulfide bond. 
     The Fab of the present invention can be obtained by treating a humanised CDR-grafted antibody of the present invention which specifically reacts with IgE, with a protease, papain. Also, the Fab can be produced by inserting DNA encoding Fab of the antibody into an expression vector for prokaryote or an expression vector for eukaryote, and introducing the vector into a prokaryote or eukaryote to express the Fab. 
     An F(ab′)2 is an antibody fragment having a molecular weight of about 100,000 and antigen binding activity, which is slightly larger than the Fab bound via a disulfide bond of the hinge region, among fragments obtained by treating IgG with a protease, pepsin. 
     The F(ab′)2 of the present invention can be obtained by treating a human CDR-grafted antibody which specifically reacts with IgE, with a protease, pepsin. Also, the F(ab′)2 can be produced by binding Fab′ described below via a thioether bond or a disulfide bond. 
     A Fab′ is an antibody fragment having a molecular weight of about 50,000 and antigen binding activity, which is obtained by cutting a disulfide bond of the hinge region of the F(ab′)2. 
     The Fab′ of the present invention can be obtained by treating the F(ab′)2 which specifically reacts with IgE, with a reducing agent, dithiothreitol. Also, the Fab′ of the present invention can be produced by inserting DNA encoding a Fab′ of a human CDR-grafted antibody of the present invention which specifically reacts with IgE into an expression vector for prokaryote or an expression vector for eukaryote, and introducing the vector into a prokaryote or eukaryote to express the Fab′. 
     An scFv is a VH-P-VL or VL-P-VH polypeptide in which one chain VH and one chain VL are linked using an appropriate peptide linker (P) of 12 or more residues and which has an antigen-binding activity. 
     The scFv of the present invention can be produced by obtaining cDNAs encoding VH and VL of a human CDR-grafted antibody which specifically reacts with IgE of the present invention, constructing DNA encoding scFv, inserting the DNA into an expression vector for prokaryote or an expression vector for eukaryote, and then introducing the expression vector into a prokaryote or eukaryote to express the scFv. 
     The Fab fragment of the present invention may be linked, directly or via a linker to a scFv. “Single chain variable fragment” or “scFv” as employed herein refers to a single chain variable fragment which is stabilized by a peptide linker between the VH and VL variable domains, for example a peptide linker with amino acid sequence which is Seq ID No. 151. The linkage to the Fab fragment can be a chemical conjugation but is most preferably a translation fusion, i.e. a genetic fusion where the sequence of each is encoded in sequence by an expression vector. The linker is therefore typically an amino acid linker as described herein. The scFv of the present invention linked to the Fab fragment may bind to a serum carrier protein in order to extend the half-life of the antibody fusion protein in vivo. Extending half-life in such a way is independent of IgE binding and may be advantageous. 
     “Serum carrier protein” as employed herein refers to any suitable plasma carrier protein to which the scFv may bind, in one example the serum carrier protein is selected from thyroxine binding protein, transthyretin, α1-acid glycoprotein, transferrin, fibrinogen and albumin, or a fragment of any thereof. Typically, the scFv binds to albumin, preferably human serum albumin. 
     Any suitable albumin binding scFv may be incorporated into the antibody fusion proteins of the invention. Suitable albumin binding domains have previously been described in the art. 
     A diabody is an antibody fragment in which scFv&#39;s having the same or different antigen binding specificity forms a dimer, and has a divalent antigen binding activity to the same antigen or two specific antigen binding activities to different antigens. 
     The diabody of the present invention, for example, a divalent diabody which specifically reacts with IgE, can be produced by obtaining cDNAs encoding VII and VL of an antibody which specifically reacts with IgE, constructing DNA encoding scFv having a polypeptide linker of 3 to 10 residues, inserting the DNA into an expression vector for prokaryote or an expression vector for eukaryote, and then introducing the expression vector into a prokaryote or eukaryote to express the diabody. 
     A dsFv is obtained by binding polypeptides in which one amino acid residue of each of VII and VL is substituted with a cysteine residue via a disulfide bond between the cysteine residues. The amino acid residue, which is substituted with a cysteine residue can be selected based on a three-dimensional structure estimation of the antibody in accordance with the method shown by Reiter et al. (Protein Engineering, 7, 697 (1994)). 
     The dsFv of the present invention can be produced by obtaining cDNAs encoding VH and VL of a human CDR-grafted antibody which specifically reacts with IgE of the present invention, constructing DNA encoding dsFv, inserting the DNA into an expression vector for prokaryote or an expression vector for eukaryote, and then introducing the expression vector into a prokaryote or eukaryote to express the dsFv. 
     A peptide comprising CDR is constituted by including at least one region of H chain and L chain CDRs. Plural CDRs can be bound directly or via an appropriate peptide linker. 
     The peptide comprising CDR of the present invention can be produced by obtaining cDNA encoding CDR of VH and VL of a human CDR-grafted antibody which specifically reacts with IgE, constructing DNA encoding CDR, inserting the DNA into an expression vector for prokaryote or an expression vector for eukaryote, and then by introducing the expression vector into a prokaryote or eukaryote to express the peptide. Also, the peptide comprising CDR can also be produced by a chemical synthesis method such as an Fmoc method (fluorenylmethoxycarbonyl method), a tBoc method (t-butyloxycarbonyl method), or the like. 
     The antibody of the present invention includes antibody derivatives in which a radioisotope, a protein, an agent or the like is chemically or genetically conjugated to the antibody of the present invention. 
     The antibody derivatives of the present invention can be produced by chemically conjugating a radioisotope, a protein or an agent to the N-terminal side or C-terminal side of an H chain or an L chain of an antibody or antibody fragment which specifically reacts with IgE, to an appropriate substituent group or side chain of the antibody or antibody fragment or to a sugar chain in the antibody or antibody fragment (Antibody Engineering Handbook, edited by Osamu Kanemitsu, published by Chijin Shokan (1994)). 
     Also, it can be genetically produced by linking a DNA encoding the antibody or the antibody fragment of the present invention which specifically reacts with IgE to other DNA encoding a protein to be bound, inserting the DNA into an expression vector, and introducing the expression vector into a host cell. 
     The radioisotope includes 131I, 125I and the like, and it can be conjugated to the antibody by, e.g., a chloramine T method. 
     The agent is preferably a low molecular weight compound. Examples include anticancer agents such as alkylating agents (e.g., nitrogen mustard, cyclophosphamide), metabolic antagonists (e.g., 5-fluorouracil, methotrexate), antibiotics (e.g., daunomycin, bleomycin, mitomycin C, daunorubicin, doxorubicin), plant alkaloids (e.g., vincristine, vinblastine, vindesine), hormone drugs (e.g., tamoxifen, dexamethasone), and the like (Clinical Oncology, edited by Japanese Society of Clinical Oncology, published by Cancer and Chemotherapy (1996)); anti-inflammatory agents such as steroid agents (e.g., hydrocortisone, prednisone), non-steroidal drugs (e.g., aspirin, indometacin), immunomodulators (e.g., aurothiomalate, penicillamine), immunosuppressing agents (e.g., cyclophosphamide, azathioprine) and antihistaminic agents (e.g., chlorpheniramine maleate, clemastine) (Inflammation and Anti-inflammatory Therapy, Ishiyaku Shuppan (1982)); and the like. The method for conjugating daunomycin to an antibody includes a method in which daunomycin and an amino group of an antibody are conjugated via glutaraldehyde, a method in which an amino group of daunomycin and a carboxyl group of an antibody are conjugated via a water-soluble carbodiimide, and the like. 
     Also, in order to inhibit cancer cells directly, a toxin such as ricin, diphtheria toxin and the like, can be used. For example, a fusion antibody with a protein can be produced by linking a cDNA encoding an antibody or antibody fragment to other cDNA encoding the protein, constructing DNA encoding the fusion antibody, inserting the DNA into an expression vector for prokaryote or an expression vector for eukaryote, and then introducing it into a prokaryote or eukaryote to express the fusion antibody. 
     Further contemplated herein are antibody fragments or antigen binding agents including fusions of binding agents, for example immunoglobulin like fragments and agents such as diabodies, scAbs, bispecific fragments, triabodies, Fab-Fv-Fv, Fab-Fv, tribody, (Fab-Fv)2-Fc, and antibody fragments or portions, such as CDRs or antibody loops including CDRs, which are grafted onto non-Ig frameworks such as fibronectin or leucine zippers, as descried in Binz et al., (2005) Nat. Biotech. 23:1257-1268, incorporated in its entirety herein. 
     Conjugated Anti-IgE Monoclonal Antibodies and Antigen Binding Agents 
     If desired, an antibody or antigen binding agent for use in the present invention may be conjugated to one or more effector molecule(s). It will be appreciated that the effector molecule may comprise a single effector molecule or two or more such molecules so linked as to form a single moiety that can be attached to the antibodies of the present invention. Where it is desired to obtain an antibody fragment linked to an effector molecule, this may be prepared by standard chemical or recombinant DNA procedures in which the antibody fragment is linked either directly or via a coupling agent to the effector molecule. Techniques for conjugating such effector molecules to antibodies are well known in the art (see, Hellstrom et al., Controlled Drug Delivery, 2nd Ed., Robinson et al., eds., 1987, pp. 623-53; Thorpe et al., 1982, Immunol. Rev., 62:119-58 and Dubowchik et al., 1999, Pharmacology and Therapeutics, 83, 67-123). Particular chemical procedures include, for example, those described in WO 93/06231, WO 92/22583, WO 89/00195, WO 89/01476 and WO 03/031581. Alternatively, where the effector molecule is a protein or polypeptide the linkage may be achieved using recombinant DNA procedures, for example as described in WO 86/01533 and EP0392745. 
     The term effector molecule as used herein includes, for example, antineoplastic agents, drugs, toxins, biologically active proteins, for example enzymes, other antibody or antibody fragments, antigen binding agents, synthetic (including PEG) or naturally occurring polymers, nucleic acids and fragments thereof e.g. DNA, RNA and fragments thereof, radionuclides, particularly radioiodide, radioisotopes, chelated metals, nanoparticles and reporter groups such as fluorescent compounds or compounds which may be detected by NMR or ESR spectroscopy. 
     Examples of effector molecules may include cytotoxins or cytotoxic agents including any agent that is detrimental to (e.g. kills) cells. Examples include combrestatins, dolastatins, epothilones, staurosporin, maytansinoids, spongistatins, rhizoxin, halichondrins, roridins, hemiasterlins, taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1-dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof. 
     Effector molecules also include, but are not limited to, antimetabolites (e.g. methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5-fluorouracil decarbazine), alkylating agents (e.g. mechlorethamine, thioepa chlorambucil, melphalan, carmustine (BSNU) and lomustine (CCNU), cyclothosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cis-dichlorodiamine platinum (II) (DDP) cisplatin), anthracyclines (e.g. daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g. dactinomycin (formerly actinomycin), bleomycin, mithramycin, anthramycin (AMC), calicheamicins or duocarmycins), and anti-mitotic agents (e.g. vincristine and vinblastine). 
     Other effector molecules may include chelated radionuclides such as 111In and 90Y, Lu177, Bismuth213, Californium252, Iridium192 and Tungsten188/Rhenium188; or drugs such as but not limited to, alkylphosphocholines, topoisomerase I inhibitors, taxoids and suramin. 
     Other effector molecules include proteins, peptides and enzymes. Enzymes of interest include, but are not limited to, proteolytic enzymes, hydrolases, lyases, isomerases, transferases. Proteins, polypeptides and peptides of interest include, but are not limited to, immunoglobulins, toxins such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin, a protein such as insulin, tumour necrosis factor, α-interferon, β-interferon, nerve growth factor, platelet derived growth factor or tissue plasminogen activator, a thrombotic agent or an anti-angiogenic agent, e.g. angiostatin or endostatin, or, a biological response modifier such as a lymphokine, interleukin-1 (IL-1), interleukin-2 (IL-2), granulocyte macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), nerve growth factor (NGF) or other growth factor and immunoglobulins. 
     Other effector molecules may include detectable substances useful for example in diagnosis. Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, radioactive nuclides, positron emitting metals (for use in positron emission tomography), and nonradioactive paramagnetic metal ions. See generally U.S. Pat. No. 4,741,900 for metal ions which can be conjugated to antibodies for use as diagnostics. Suitable enzymes include horseradish peroxidase, alkaline phosphatase, beta-galactosidase, or acetylcholinesterase; suitable prosthetic groups include streptavidin, avidin and biotin; suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride and phycoerythrin; suitable luminescent materials include luminol; suitable bioluminescent materials include luciferase, luciferin, and aequorin; and suitable radioactive nuclides include 125I, 131I, 111In and 99Tc. 
     In another example the effector molecule may increase the half-life of the antibody in vivo, and/or reduce immunogenicity of the antibody and/or enhance the delivery of an antibody across an epithelial barrier to the immune system. Examples of suitable effector molecules of this type include polymers, albumin, albumin binding proteins or albumin binding compounds such as those described in WO05/117984. 
     In one embodiment a half-life provided by an effector molecule which is independent of IgE or an anti-human IgE antibody is advantageous. 
     Where the effector molecule is a polymer it may, in general, be a synthetic or a naturally occurring polymer, for example an optionally substituted straight or branched chain polyalkylene, polyalkenylene or polyoxyalkylene polymer or a branched or unbranched polysaccharide, e.g. a homo- or hetero-polysaccharide. 
     Specific optional substituents, which may be present on the above-mentioned synthetic polymers, include one or more hydroxy, methyl or methoxy groups. 
     Specific examples of synthetic polymers include optionally substituted straight or branched chain poly(ethyleneglycol), poly(propyleneglycol) poly(vinylalcohol) or derivatives thereof, especially optionally substituted poly(ethyleneglycol) such as methoxypoly(ethyleneglycol) or derivatives thereof. 
     Specific naturally occurring polymers include lactose, amylose, dextran, glycogen or derivatives thereof. 
     In one embodiment the polymer is albumin or a fragment thereof, such as human serum albumin or a fragment thereof. In one embodiment the polymer is a PEG molecule. 
     “Derivatives” as used herein in regard to conjugates is intended to include reactive derivatives, for example thiol-selective reactive groups such as maleimides and the like. The reactive group may be linked directly or through a linker segment to the polymer. It will be appreciated that the residue of such a group will in some instances form part of the product as the linking group between the antibody fragment and the polymer. 
     The size of the natural or synthetic polymer may be varied as desired, but will generally be in an average molecular weight range from 500 Da to 50000 Da, for example from 5000 to 40000 Da such as from 20000 to 40000 Da. The polymer size may in particular be selected on the basis of the intended use of the product for example ability to localize to certain tissues such as tumors or extend circulating half-life (for review see Chapman, 2002, Advanced Drug Delivery Reviews, 54, 531-545). Thus, for example, where the product is intended to leave the circulation and penetrate tissue, for example for use in the treatment of a tumour, it may be advantageous to use a small molecular weight polymer, for example with a molecular weight of around 5000 Da. For applications where the product remains in the circulation, it may be advantageous to use a higher molecular weight polymer, for example having a molecular weight in the range from 20000 Da to 40000 Da. 
     Suitable polymers include a polyalkylene polymer, such as a poly(ethyleneglycol) or, especially, a methoxypoly(ethyleneglycol) or a derivative thereof, and especially with a molecular weight in the range from about 15000 Da to about 40000 Da. 
     In one example antibodies for use in the present invention are attached to poly(ethyleneglycol) (PEG) moieties. In one particular example the antibody is an antibody fragment and the PEG molecules may be attached through any available amino acid side-chain or terminal amino acid functional group located in the antibody fragment, for example any free amino, imino, thiol, hydroxyl or carboxyl group. Such amino acids may occur naturally in the antibody fragment or may be engineered into the fragment using recombinant DNA methods (see for example U.S. Pat. Nos. 5,219,996; 5,667,425; WO98/25971, WO2008/038024). In one example the antibody molecule of the present invention is a modified Fab fragment wherein the modification is the addition to the C-terminal end of its heavy chain one or more amino acids to allow the attachment of an effector molecule. Suitably, the additional amino acids form a modified hinge region containing one or more cysteine residues to which the effector molecule may be attached. Multiple sites can be used to attach two or more PEG molecules. 
     Suitably PEG molecules are covalently linked through a thiol group of at least one cysteine residue located in the antibody fragment. Each polymer molecule attached to the modified antibody fragment may be covalently linked to the sulphur atom of a cysteine residue located in the fragment. The covalent linkage will generally be a disulphide bond or, in particular, a sulphur-carbon bond. Where a thiol group is used as the point of attachment appropriately activated effector molecules, for example thiol selective derivatives such as maleimides and cysteine derivatives may be used. An activated polymer may be used as the starting material in the preparation of polymer-modified antibody fragments as described above. 
     The activated polymer may be any polymer containing a thiol reactive group such as an α-halocarboxylic acid or ester, e.g. iodoacetamide, an imide, e.g. maleimide, a vinyl sulphone or a disulphide. Such starting materials may be obtained commercially (for example from Nektar, formerly Shearwater Polymers Inc., Huntsville, Ala., USA) or may be prepared from commercially available starting materials using conventional chemical procedures. Particular PEG molecules include 20K methoxy-PEG-amine (obtainable from Nektar, formerly Shearwater; Rapp Polymere; and SunBio) and M-PEG-SPA (obtainable from Nektar, formerly Shearwater). 
     In one embodiment, the antibody is a modified Fab fragment, Fab′ fragment or diFab which is PEGylated, i.e. has PEG (poly(ethyleneglycol)) covalently attached thereto, e.g. according to the method disclosed in EP 0948544 or EP1090037 [see also “Poly(ethyleneglycol) Chemistry, Biotechnical and Biomedical Applications”, 1992, J. Milton Harris (ed), Plenum Press, New York, “Poly(ethyleneglycol) Chemistry and Biological Applications”, 1997, J. Milton Harris and S. Zalipsky (eds), American Chemical Society, Washington D.C. and “Bioconjugation Protein Coupling Techniques for the Biomedical Sciences”, 1998, M. Aslam and A. Dent, Grove Publishers, New York; Chapman, A. 2002, Advanced Drug Delivery Reviews 2002, 54:531-545]. In one example PEG is attached to a cysteine in the hinge region. In one example, a PEG modified Fab fragment has a maleimide group covalently linked to a single thiol group in a modified hinge region. A lysine residue may be covalently linked to the maleimide group and to each of the amine groups on the lysine residue may be attached a methoxypoly(ethyleneglycol) polymer having a molecular weight of approximately 20,000 Da. The total molecular weight of the PEG attached to the Fab fragment may therefore be approximately 40,000 Da. 
     Particular PEG molecules include 2-[3-(N-maleimido)propionamido]ethyl amide of N,N′-bis(methoxypoly(ethylene glycol) MW 20,000) modified lysine, also known as PEG2MAL40K (obtainable from Nektar, formerly Shearwater). 
     Alternative sources of PEG linkers include NOF who supply GL2-400MA3 (wherein m in the structure below is 5) and GL2-400MA (where m is 2) and n is approximately 450: 
     
       
         
         
             
             
         
       
     
     That is to say each PEG is about 20,000 Da. 
     Thus in one embodiment the PEG is 2,3-Bis(methylpolyoxyethylene-oxy)-1-{[3-(6-maleimido-1-oxohexyl)amino]propyloxy}hexane (the 2 arm branched PEG, —CH2) 3NHCO(CH2)5-MAL, Mw 40,000 known as SUNBRIGHT GL2-400MA3. 
     Further alternative PEG effector molecules of the following type: 
     
       
         
         
             
             
         
       
     
     are available from Dr Reddy, NOF and Jenkem. 
     In one embodiment there is provided an antibody of the invention which is PEGylated (for example with a PEG described herein), attached through a cysteine amino acid residue at or about amino acid 226 in the chain, for example amino acid 226 of the heavy chain (by sequential numbering). 
     In one embodiment the present disclosure provides a Fab′PEG molecule comprising one or more PEG polymers, for example 1 or 2 polymers such as a 40 kDa polymer or polymers. 
     Fab′-PEG molecules according to the present disclosure may be particularly advantageous in that they have a half-life independent of the Fc fragment. In one example the present invention provides a method treating a disease ameliorated by modulating human IgE biological activity comprising administering a therapeutically effective amount of an anti-IgE antibody or antigen binding agent thereof wherein the antibody or antigen binding agent thereof has a half-life that is independent of Fc binding to IgE. 
     In one embodiment there is provided a Fab′ conjugated to a polymer, such as a PEG molecule, a starch molecule or an albumin molecule. 
     In one embodiment there is provided a scFv conjugated to a polymer, such as a PEG molecule, a starch molecule or an albumin molecule. 
     In one embodiment the antibody or fragment is conjugated to a starch molecule, for example to increase the half-life. Methods of conjugating starch to a protein as described in U.S. Pat. No. 8,017,739 incorporated herein by reference. 
     Polynucleotides 
     The present invention also provides an isolated DNA sequence encoding the heavy and/or light chain(s) of an antibody molecule of the present invention. Suitably, the DNA sequence encodes the heavy or the light chain of an antibody molecule of the present invention. The DNA sequence of the present invention may comprise synthetic DNA, for instance produced by chemical processing, cDNA, genomic DNA or any combination thereof. 
     DNA sequences which encode an antibody molecule of the present invention can be obtained by methods well known to those skilled in the art. For example, DNA sequences coding for part or all of the antibody heavy and light chains may be synthesised as desired from the determined DNA sequences or on the basis of the corresponding amino acid sequences. 
     DNA coding for acceptor framework sequences is widely available to those skilled in the art and can be readily synthesised synthesized on the basis of their known amino acid sequences. 
     Standard techniques of molecular biology may be used to prepare DNA sequences coding for the antibody molecule of the present invention. Desired DNA sequences may be synthesized completely or in part using oligonucleotide synthesis techniques. Site-directed mutagenesis and polymerase chain reaction (PCR) techniques may be used as appropriate. 
     The present invention also relates to a cloning or expression vector comprising one or more DNA sequences of the present invention. Accordingly, provided is a cloning or expression vector comprising one or more DNA sequences encoding an antibody of the present invention. Suitably, the cloning or expression vector comprises two DNA sequences, encoding the light chain and the heavy chain of the antibody molecule of the present invention, respectively and suitable signal sequences. In one example the vector comprises an intergenic sequence between the heavy and the light chains (see WO03/048208). 
     General methods by which the vectors may be constructed, transfection methods and culture methods are well known to those skilled in the art. In this respect, reference is made to “Current Protocols in Molecular Biology”, 1999, F. M. Ausubel (ed), Wiley Interscience, New York and the Maniatis Manual produced by Cold Spring Harbour Publishing. 
     Host Cells Expressing Anti-IgE Antibodies or Fragments Thereof 
     Also provided is a host cell comprising one or more cloning or expression vectors comprising one or more DNA sequences encoding an antibody of the present invention. Any suitable host cell/vector system may be used for expression of the DNA sequences encoding the antibody molecule of the present invention. Bacterial, for example  E. coli , and other microbial systems may be used or eukaryotic, for example mammalian, host cell expression systems may also be used. Suitable mammalian host cells include CHO, myeloma or hybridoma cells. 
     Suitable types of Chinese Hamster Ovary (CHO cells) for use in the present invention may include CHO and CHO-Kl cells including dhfr− CHO cells, such as CHO-DG44 cells and CHO-DXB11 cells and which may be used with a DHFR selectable marker or CHOK1-SV cells which may be used with a glutamine synthetase selectable marker. Other cell types of use in expressing antibodies include lymphocytic cell lines, e.g., NSO myeloma cells and SP2 cells, COS cells. Other suitable cells may include human embryonic kidney (hek) fibroblasts, for example hek293F and ExpiHek cells, which are known in the art. 
     CHO is preferred for full-length Ab of the invention, given this is standard host for the production of omalizumab (in one embodiment giving the antibodies of the invention the standard glycosylation pattern of omalizumab) [see also WO 2013/181577]. 
     Production of Anti-IgE Antibodies or Fragments Thereof 
     The present invention also provides a process for the production of an antibody molecule according to the present invention comprising culturing a host cell containing a vector of the present invention under conditions suitable for leading to expression of protein from DNA encoding the antibody molecule of the present invention, and isolating the antibody molecule. 
     The antibody molecule may comprise only a heavy or light chain polypeptide, in which case only a heavy chain or light chain polypeptide coding sequence needs to be used to transfect the host cells. For production of products comprising both heavy and light chains, the cell line may be transfected with two vectors, a first vector encoding a light chain polypeptide and a second vector encoding a heavy chain polypeptide. Alternatively, a single vector may be used, the vector including sequences encoding light chain and heavy chain polypeptides. 
     There is a provided a process for culturing a host cell and expressing an antibody or fragment thereof, isolating the latter and optionally purifying the same to provide an isolated antibody or fragment. In one embodiment the process further comprises the step of conjugating an effector molecule to the isolated antibody or fragment, for example conjugating to a PEG polymer in particular as described herein. 
     In one embodiment there is provided a process for purifying an antibody (in particular an antibody or fragment according to the invention) comprising the steps: performing anion exchange chromatography in non-binding mode such that the impurities are retained on the column and the antibody is eluted. 
     In one embodiment the purification employs affinity capture on a Protein A column, and then titration. On one embodiment, the purification employs affinity capture on a Protein G column, and then HPLC titration. On one embodiment, the purification employs affinity capture on an IgE column, and then titration. 
     In one embodiment the purification employs cibacron blue or similar for purification of albumin fusion or conjugate molecules. 
     Suitable ion exchange resins for use in the process include Q.FF resin (supplied by GE-Healthcare). The step may, for example be performed at a pH about 8. 
     The process may further comprise an initial capture step employing cation exchange chromatography, performed for example at a pH of about 4 to 5, such as 4.5. The cation exchange chromatography may, for example employ a resin such as CaptoS resin or SP sepharose FF (supplied by GE-Healthcare). The antibody or fragment can then be eluted from the resin employing an ionic salt solution such as sodium chloride, for example at a concentration of 200 mM. 
     Thus the chromatograph step or steps may include one or more washing steps, as appropriate. 
     The purification process may also comprise one or more filtration steps, such as a diafiltration step or HPLC filtration step. 
     Thus in one embodiment there is provided a purified anti-IgE antibody or fragment, for example a humanised antibody or fragment, in particular an antibody or fragment according to the invention, in substantially purified from, in particular free or substantially free of endotoxin and/or host cell protein or DNA. 
     Purified from as used supra is intended to refer to at least 90% purity, such as 91, 92, 93, 94, 95, 96, 97, 98, 99% w/w or purer. 
     Substantially free of endotoxin is generally intended to refer to an endotoxin content of 1 EU per mg antibody product or less such as 0.5 or 0.1 EU per mg product. 
     Substantially free of host cell protein or DNA is generally intended to refer to host cell protein and/or DNA content 400 μg per mg of antibody product or less such as 100 μg per mg or less, in particular 20 μg per mg, as appropriate. 
     Pharmaceutical Compositions 
     As the antibodies of the present invention are useful in the treatment and/or prophylaxis of a pathological condition, the present invention also provides a pharmaceutical or diagnostic composition comprising an antibody or antigen binding agent of the present invention in combination with one or more of a pharmaceutically acceptable excipient, diluent, or carrier. Accordingly, provided is the use of an antibody or antigen binding agent of the invention for the manufacture of a medicament. The composition will usually be supplied as part of a sterile, pharmaceutical composition that will normally include a pharmaceutically acceptable carrier. A pharmaceutical composition of the present invention may additionally comprise a pharmaceutically-acceptable excipient. 
     The present invention also provides a process for preparation of a pharmaceutical or diagnostic composition comprising adding and mixing the antibody or antigen binding agent of the present invention together with one or more of a pharmaceutically acceptable excipient, diluent, or carrier. 
     The antibody or antigen binding agent may be the sole active ingredient in the pharmaceutical or diagnostic composition or may be accompanied by other active ingredients including other antibody ingredients or non-antibody ingredients such as steroids or other drug molecules, in particular drug molecules whose half-life is independent of IgE binding. 
     The pharmaceutical compositions suitably comprise a therapeutically effective amount of the antibody or antigen binding agent of the invention. The term “therapeutically effective amount” as used herein refers to an amount of a therapeutic agent needed to treat, ameliorate, or prevent a targeted disease or condition, or to exhibit a detectable therapeutic or preventative effect. For any disclosed antibody or antigen binding agent, the therapeutically effective amount can be estimated initially either in cell culture assays or in animal models, usually in rodents, rabbits, dogs, pigs or primates. The animal model may also be used to determine the appropriate concentration range and route of administration. Such information can then be used to determine useful doses and routes for administration in humans. 
     The precise therapeutically effective amount for a human subject will depend upon the severity of the disease state, the general health of the subject, the age, weight and gender of the subject, diet, time and frequency of administration, drug combination(s), reaction sensitivities and tolerance/response to therapy. This amount can be determined by routine experimentation and is within the judgement of the clinician. Generally, a therapeutically effective amount will be from 0.01 mg/kg to 500 mg/kg, for example 0.1 mg/kg to 200 mg/kg, such as 100 mg/Kg. Pharmaceutical compositions may be conveniently presented in unit dose forms containing a predetermined amount of an active agent of the invention per dose. 
     Therapeutic doses of the antibodies or antigen binding agents according to the present disclosure show no apparent toxicology effects in vivo. 
     Advantageously, the levels of IgE activity in vivo may be maintained at an appropriately reduced level by administration of sequential doses of the antibody or binding agent according to the disclosure. 
     Compositions may be administered individually to a patient or may be administered in combination (e.g. simultaneously, sequentially, or separately) with other agents, drugs or hormones. 
     A pharmaceutical composition may also contain a pharmaceutically acceptable carrier for administration of the antibody or antigen binding agent. The carrier should not itself induce the production of antibodies harmful to the individual receiving the composition and should not be toxic. Suitable carriers may be large, slowly metabolised macromolecules such as proteins, polypeptides, liposomes, polysaccharides, polylactic acids, polyglycolic acids, polymeric amino acids, amino acid copolymers and inactive virus particles. 
     Pharmaceutically acceptable salts can be used, for example mineral acid salts, such as hydrochlorides, hydrobromides, phosphates and sulphates, or salts of organic acids, such as acetates, propionates, malonates and benzoates. 
     Pharmaceutically acceptable carriers in therapeutic compositions may additionally contain liquids such as water, saline, glycerol and ethanol. Additionally, auxiliary substances, such as wetting or emulsifying agents or pH buffering substances, may be present in such compositions. Such carriers enable the pharmaceutical compositions to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries and suspensions, for ingestion by the patient. 
     Preferred forms for administration include forms suitable for parenteral administration, e.g. by injection or infusion, for example by bolus injection or continuous infusion. Where the product is for injection or infusion, it may take the form of a suspension, solution or emulsion in an oily or aqueous vehicle and it may contain formulatory agents, such as suspending, preservative, stabilising and/or dispersing agents. Alternatively, the antibody molecule may be in dry form, for reconstitution before use with an appropriate sterile liquid. 
     Once formulated, the compositions of the invention can be administered directly to the subject. The subjects to be treated can be animals. However, it is preferred that the compositions are adapted for administration to human subjects. 
     The pharmaceutical compositions of this invention may be administered by any number of routes including, but not limited to, oral, intravenous, intramuscular, intra-arterial, intramedullary, intrathecal, intraventricular, transdermal, transcutaneous (for example, see WO98/20734), subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual, intravaginal or rectal routes. Hyposprays may also be used to administer the pharmaceutical compositions of the invention. Typically, the therapeutic compositions may be prepared as injectables, either as liquid solutions or suspensions. Solid forms suitable for solution in, or suspension in, liquid vehicles prior to injection may also be prepared. 
     Direct delivery of the compositions will generally be accomplished by injection, subcutaneously (in particular), intraperitoneally, intravenously or intramuscularly, or delivered to the interstitial space of a tissue. The compositions can also be administered into a lesion. Dosage treatment may be a single dose schedule or a multiple dose schedule. 
     It will be appreciated that the active ingredient in the composition will be an antibody molecule. As such, it will be susceptible to degradation in the gastrointestinal tract. Thus, if the composition is to be administered by a route using the gastrointestinal tract, the composition will need to contain agents which protect the antibody from degradation but which release the antibody once it has been absorbed from the gastrointestinal tract. 
     A thorough discussion of pharmaceutically acceptable carriers is available in Remington&#39;s Pharmaceutical Sciences (Mack Publishing Company, N.J. 1991). 
     Structural-Functional Properties 
     In one aspect of the present invention, the antibody or antigen binding agent binds to free and FcεRI bound human IgE. When the antibody (or antigen binding agent) of the invention binds to the FcεRI bound human IgE, it stabilizes a conformation of IgE. In such stabilized conformation, the IgE has a binding affinity for FcεRI or omalizumab (or fragments thereof) weaker than in the absence of the antibody or antigen binding agent of the present invention and wherein the FcεRI bound human IgE dissociates from FcεRI. Preferably, the IgE, upon dissociation from the FcεRI, remains bound to the antibody or antigen binding agent as described herein. As it will be shown hereinafter (for instance in Example 1 and  FIG. 2 ), the antibody or antigen binding agent of the invention binds to the IgE in a conformation which is different from the conformation IgE has when bound to omalizumab. 
     Without wishing to be bound by theory, the antibody of the present invention causes the IgE to adopt a partially bent conformation ( FIGS. 2C and 5C &amp;D), hence, unbending from the free or FcεRI bound IgE structures. Such unbending compromises the ability of the IgE to bind or keep binding the FcεRI. As it will be shown in the example section, because the antibody of the present invention competes with the binding site on IgE for binding to the FcεRI, it is believed that the antibody may be capable of forming a complex with the IgE bound to the FcεRI altering the structure of the IgE when bound to the FcεRI and dissociating it from the FcεRI. Antibodies according to the present invention which possess these properties are those described herein such as antibodies or antigen binding agent comprising:
         a. a heavy chain variable region comprising Seq ID No.: 1 and a light chain variable region comprising:
           i. Seq ID No.: 109; or   ii. Seq ID No.: 113; or   iii. Seq ID No.: 121; or   iv. Seq ID No.: 132; or   v. Seq ID NO. 139; or   
           b. Seq ID No.: 5 and
           i. Seq ID No., wherein S77 and S79 are replaced by Q;   ii. Seq ID No.: 117 or   iii. Seq ID No.: 125; or   iv. Seq ID No.: 136; or   v. Seq ID No.: 143.   
               

     Anti-IgE antibodies or antigen binding agents possessing such properties contact or contact and are specific for an epitope comprising, with reference to SEQ ID NO: 108, residues T373, W374, S375, R376, A377, S378, G379, P381, Q417, C418, R419, P426, R427, A428 of a Cε3 domain and residues D278 and T281 of a Cε2 domain of human IgE. 
     The antibodies of the present invention possess at several positions methionine residues. Oxidation of methionine residues is among the most common protein degradation pathway. The antibodies of the present invention where methionine residues have been introduced at position S64 and S71, with reference to Seq ID No. 20, may undergo complete oxidation without affecting the ability of the antibodies to accelerate dissociation of the IgE-Fc:sFcRIα complex. 
     Therefore, the present invention also provides for an anti-IgE antibody, or antigen-binding agent, which comprises a heavy chain variable region which has an amino acid sequence which is Seq ID No. 1 and a light chain variable region which comprises a CDR-L2 and a FW-L3, wherein the light chain variable region has an amino acid sequence which is Seq ID No. 20, except that the CDR-L2 has an amino acid sequence which is Seq ID No. 50 and that the FW-L3 has an amino acid sequence which is Seq ID No. 131 or 138, wherein methionine residues at positions 64 and/or 71, with reference to Seq ID No. 20, are oxidised. 
     The present invention also provides for an anti-IgE antibody, or antigen-binding agent, which comprises a heavy chain variable region which has an amino acid sequence which is Seq ID No. 1 and a light chain variable region which has an amino acid sequence which is Seq ID No. 132 or 139, wherein methionine residues at positions 64 and/or 71, with reference to Seq ID No. 132 or 139, are oxidised. 
     Further methionine residues in the antibodies of the present invention may be oxidised without affecting the ability of the antibodies to accelerate dissociation of the IgE-Fc:sFcRIα complex. The invention will now be further described by way of examples with references to embodiments illustrated in the accompanying drawings. 
     EXAMPLES 
     Example 1: Structure of a Mutant of the Therapeutic Anti-IgE Antibody Omalizumab Bound to IgE-Fc Reveals its Mechanism of Action 
     Abstract 
     Immunoglobulin E and its interactions with receptors FcεRI and CD23 play a central role in allergic disease. Omalizumab, a clinically-approved therapeutic antibody, inhibits the interaction between IgE and FcεRI, preventing mast cell and basophil activation, and blocks IgE binding to CD23. We solved the crystal structure of the 2:1 complex between an omalizumab-derived Fab and IgE-Fc, with one Fab bound to each Cε3 domain (but only one of the Fabs bound to a Cε2 domain). Although free IgE-Fc is predominantly acutely bent in solution, in the complex it is only partially bent, precluding interaction with FcεRI; CD23 binding is inhibited sterically due to overlapping binding sites on each Cε3 domain. Solution state interaction analyses demonstrate the orthosteric and allosteric basis for the inhibition of both receptor interactions and, together with the structure, reveal how omalizumab (and particularly the described omalizumab mutants) may accelerate dissociation of receptor-bound IgE from FcεRI, exploiting the intrinsic dynamics and allosteric potential of IgE. 
     INTRODUCTION 
     Immunoglobulin E (IgE) antibodies play a crucial role in allergic disease, binding to allergens through their Fab arms and expressing their effector functions by binding to receptors for the Fc region 1 . The two principal IgE receptors are FcεRI and CD23/FcεRII, commonly referred to as the high- and low-affinity receptors respectively. On mast cells and basophils, IgE binds to FcεRI so tightly (K D ≈10 −10  M −1 ) that such cells are sensitized with pre-bound IgE, requiring only the presence of an allergen to cross-link IgE/FcεRI complexes and elicit an immediate reaction. CD23 is a homo-trimer and thus the intrinsically lower affinity of each IgE-binding, C-type lectin-like “head” domain (K D ≈10 −7  M −1 ) can be enhanced by an avidity effect when binding to aggregated IgE in immune complexes, nearly matching that of FcεRI for IgE 2 . CD23 expressed on B cells is involved in IgE regulation, and expression on airway and gut epithelial cells mediates transcytosis of IgE/allergen complexes 1,2 . FcεRI and CD23 are also both expressed on a range of antigen-presenting cells. Thus, IgE-receptor interactions are involved in multiple aspects of the allergic response and IgE is a long-standing target for therapeutic intervention 3 . 
     The Fc region of IgE comprises a disulphide-linked dimer of three domains: Cε2, Cε3 and Cε4. Early FRET studies of a chimeric IgE 4,5 , and X-ray solution scattering studies of IgE-Fc 6 , indicated a compact, bent structure, and the crystal structure of IgE-Fc later revealed an acutely and asymmetrically bent conformation, with the (Cε2) 2  domain pair folded back onto the Cε3 and Cε4 domains 7 . The bend, defined as the angle between the local two-fold axis of the (Cε2) 2  domain pair and that of Fcε3-4 (the region comprising only the Cε3 and Cε4 domains) was found to become even more acute (62° to 54°) in the crystal structure of IgE-Fc bound to sFcεRIα, the soluble extracellular domains of the IgE-binding α-chain of the receptor 8 . Recent FRET studies with N- and C-terminally labelled IgE-Fc confirmed this enhanced bend upon sFcεRIα binding 9 . 
     The FcεRI binding site spans both Cε3 domains in the Cε2-proximal region 8,10 , although the Cε2 domain is not directly involved; the engagement of both chains accounts for the 1:1 binding stoichiometry. In contrast, two CD23 molecules bind to IgE-Fc, one in each chain, and at the other, Cε4-proximal end of the Cε3 domain 11,12,13.  CD23 binding also causes a conformational change in IgE-Fc 14 , but not one that significantly affects the bend 9 . However, the relatively “closed” disposition of the Cε3 domains in the complex with the soluble head domain of CD23 (sCD23), compared with free IgE-Fc, is incompatible with the more “open” arrangement of these domains that is required for FcεRI binding. This partly explains the mutual exclusion of FcεRI and CD23 binding 11,12,  although other factors such as local conformational changes and modifications of conformational dynamics 15  also likely contribute to the allosteric communication between the two receptor-binding sites 2 . 
     A more extreme degree of flexibility in IgE-Fc was recently discovered through studies of a complex with an anti-IgE-Fc Fab, termed aεFab 16 . Two aεFab molecules bound to IgE-Fc in a symmetrical manner, one on each Cε3 domain, trapping a fully extended conformation in which the local two-fold axes of the (Cε2) 2  domains and Fcε3-4 region were virtually coincident. 
     Analysis of the complex formation in solution, together with molecular dynamics simulations of free IgE-Fc, suggested that the (Cε2) 2  domain pair could “flip” over from one side of the Fcε3-4 region to the other 16 . The IgE-Fc conformation stabilized by this anti-IgE antibody is incompatible with FcεRI binding, explaining its inhibitory activity. 
     Omalizumab is an anti-IgE monoclonal IgG1 antibody that is approved for therapeutic use (Xolair®, Novartis) 17 . It binds to free IgE and inhibits both FcεRI and CD23 binding; the site of binding has been mapped to the Cε3 domain by peptide inhibition and molecular modelling 18,19  but its mechanism of action is unknown. However, binding to FRET-labelled IgE-Fc indicated a slight degree of unbending 9  and thus the potential for allosteric rather than direct inhibition. 
     Recently a type of inhibitor was discovered that actively disrupted preformed IgE/FcεRI complexes: a Designed Ankyrin Repeat Protein (DARPin) was found to bind to the Cε3 domain of receptor-bound IgE and accelerate its dissociation from FcεRI 20 . The crystal structure of the 2:1 complex of DARPin E2_79 with an Fcε3-4 molecule constrained by an engineered disulphide bond revealed the nature and location of the binding site, but left its mechanism of action unclear. It was subsequently reported that omalizumab could similarly facilitate dissociation of FcεRI-bound IgE, although only at very high concentrations that were substantially greater than those achieved in therapeutic use 21,22 . 
     We report here the crystal structure of the complex between IgE-Fc and a new antibody fragment, a Fab derived from omalizumab (omalizumab Fab3) that contains three point mutations distal to the antigen (IgE-Fc) binding complementarity determining regions (CDRs). The mutations are S81R, Q83R and L158P with reference to Seq ID No. 125 (or S77R, Q79R and L154P with reference to Seq ID No. 129). The structure of the complex reveals the mechanism of action of omalizumab, and solution studies demonstrate that this mechanism exploits the intrinsic dynamics of IgE. 
     Results 
     Despite extensive efforts, crystallization trials for IgE-Fc in complex with the omalizumab Fab resulted in selective crystallization of the Fab fragment only. Others have reported similar failure to crystallize this complex 23 . We therefore designed a new antibody, a Fab derived from omalizumab, with three point mutations, two in the V L  domain framework region (Ser81 Arg, Gln83Arg) and one in the Cκ domain (Leu158Pro) (Seq. ID No 125, PDB numbering) ( FIG. 1 ), with the purpose of disrupting favorable crystal contacts observed in the omalizumab Fab crystal structure (results to be reported elsewhere). We term this omalizumab-derived Fab “omalizumab Fab3”. 
     Overall Structure of the IgE-Fc/Omalizumab Fab3 Complex 
     We determined the crystal structure of the complex between IgE-Fc and omalizumab Fab3 to 3.7 Å resolution ( FIG. 2A ). Two omalizumab Fab3 molecules (Fab 1  and Fab 2 ) bind to an asymmetric, partially bent IgE-Fc molecule, each Fab engaging one Cε3 domain ( FIGS. 2B &amp;C). Fab 1  engages the Cε3 domain of IgE-Fc chain B, while Fab 2  engages the Cε3 domain of IgE-Fc chain A. Due to the partially bent conformation of IgE-Fc in the complex, the light chain of Fab 2  also forms a minor interaction with the Cε2 domain from IgE-Fc chain B (see later in this example for details of this interaction). 
     The Interface Between IgE-Fc and Omalizumab Fab3 
     Each omalizumab Fab3 molecule engages one edge of the exposed face of the Cε3 domain (C, C′, F and G strands, and base of the FcεRI receptor-binding FG loop). Both the heavy and light chain of omalizumab Fab3 are involved, the former contributing˜60% to an interface area of ˜715 Å 2  ( FIGS. 2&amp;3 ). 
     The omalizumab Fab3 heavy chain (Seq. ID No. 5) contacts, which differ slightly between the two interfaces, may be summarised as follows: Gly32 and Tyr33 (CDRH1) form van der Waals interactions with Ala377 and Ser378 (Cε3) (IgE-Fc sequence as shown in Seq. ID No 108 and  FIG. 16 ), while Tyr54 (CDRH2) contacts Gly379-Pro381 (Cε3). The CDRH3 residues contribute the largest contact area, and undergo a significant conformational change upon complex formation, when compared with unbound Fab structures (unpublished results, 19,23 ). CDRH3 residues Ser100, His101, Tyr102 and Trp 106 all form van der Waals interactions with Cε3 domain residues that include Ser375-Gly379, G1n417 and Arg419 (Cε3). However, the most striking feature of this part of the interface is the interaction with Phe103 (CDRH3). Phe103 is mostly buried in a pocket created by Thr373, the Trp374 main chain, Ser375, G1n417 and Arg419 (Cε3), and forms a cation/π stacking interaction with Arg419 ( FIG. 3 ). 
     Arg419 (Cε3) also plays a key role in the interaction with the omalizumab Fab3 light chain (Seq. ID No. 125) ( FIG. 3 ). Arg419 (Cε3) is within hydrogen bonding distance of the Tyr31 (CDRL1) and Asp32 (CDRL1) main chain carbonyl oxygen atoms, in addition to contacting the Asp32, Asp34 and Tyr36 side chains (forming a hydrogen bond with the Tyr36 hydroxyl group). Asp32 also forms van der Waals interactions with Thr373 and Thr421 (Cε3). By contrast, only two CDRL2 residues contribute to the interface: Tyr53 (CDRL2) contacts Gln417 (Cε3), and both Tyr53 and Tyr57 form van der Waals interactions with Met430 (Cε3); Tyr57 also forms a hydrogen bond with the Met430 backbone. As for the heavy chain interaction, there are slight differences in the light chain contacts for Fab 1  and Fab 2 . 
     CDR Contact Residues on Omalizumab Fab3 Numbering—in Format (Pdb/Kabat/Chothia). Heavy Chain Sequence: Seq. ID No 5; Light Chain Sequence: Seq. ID No 125 
     CDRH1: Ser (31/31/31), Gly (32/32/31a), Tyr (33/33/32) 
     CDRH2: Tyr (54/53/53) 
     CDRH3: Ser (100/96/96), His (101/97/97), Tyr (102/98/98), Phe (103/99/99), Trp (106/101B/101B) 
     CDRL1: Asp (30/27C/30), Tyr (31/27D/30A), Asp (32/28/30B), Gly (33/29/30C), Asp (34/30/30D), Tyr (36/32/32) 
     CDRL2: Tyr (53/49/49), Ser (56/52/52), Tyr (57/53/53), Ser (60/56/56) 
     CDRL1 and CDRH3 have the most residues involved in the interaction, and therefore characterise how omalizumab binds and orients itself relative to IgE-Fc. CDRL3 is not involved in binding to IgE-Fc. 
     A Comparison of the Omalizumab Fab3 Interface with Other Anti-IgE Complexes 
     The binding sites on the Cε3 domain for omalizumab Fab3 and the recently described DARPin E2_79 20  overlap ( FIG. 4 ), and are of similar size at ˜715 Å 2  and ˜753| 2  respectively. The Cε3 domain residues shared between the two interfaces include Ser375-Gly379, Gln417, Arg419, Arg427 and Met430, but while omalizumab Fab3 forms more intimate contacts with the receptor-binding Cε3 FG loop, the DARPin E2_79 interface extends in the opposite direction to include the Cε3-4 domain linker 
     The overlapping binding sites of omalizumab Fab3 and DARPin E2_79 differ markedly from the interface recently described for the omalizumab Fab3, which captured IgE-Fc in a fully extended conformation 16  ( FIG. 5 ). Not only is the omalizumab Fab3 interface area approximately double that of omalizumab Fab3 and DARPin E2_79, at ˜1400 Å 2 , but omalizumab Fab3 engages IgE-Fc at a site centred on Arg393 in Cε3, and also contacts residues in the Cε2 domain and the Cε2-Cε3 linker 16 . The crystal structure of another anti-IgE antibody Fab, MEDI4212, in a 2:1 complex with Fcε3-4 reveals yet another site for antibody engagement within the Cε3 domain, this one involving the N-linked oligosaccharide moiety at Asn394 24 . 
     IgE-Fc Adopts a Partially Bent Conformation when Bound to Omalizumab Fab3 
     IgE-Fc is predominantly bent in solution 5,6,9,25,26,27,28,  and the crystal structure for free IgE-Fc revealed an acutely bent (62°), asymmetric conformation, in which the (Cε2) 2  domain pair folded back onto the Cε3 and Cε4 domains ( FIGS. 5A &amp;B), the Cε2 domain of one chain (chain B) contacting the Cε4 domain of the other (chain A) 7,8 . IgE-Fc becomes even more acutely bent (54°) upon FcεRIα engagement 8,9 , and the associated conformational changes involve rotation of the Cε3 domain of chain A together with the (Cε2) 2  domain pair, as a rigid unit, away from the Cε3 domain of chain B 8 . 
     In contrast to the omalizumab Fab3 complex, in which IgE-Fc adopts a fully extended, linear conformation 16 , IgE-Fc adopts a partially bent conformation in the omalizumab Fab3 complex ( FIGS. 2C and 5C &amp;D), consistent with earlier FRET studies which revealed that omalizumab caused IgE-Fc to unbend 9 . The site to which Fab 1  binds is exposed in free, acutely bent IgE-Fc, but further unbending of IgE-Fc, to just over 90°, is required to render the site occupied by Fab 2  accessible. This unbending of IgE-Fc in the omalizumab Fab3 complex is associated with opening of both Cε3 domains, to create an almost symmetrical Fcε3-4 region ( FIG. 2B ). The (Cε2) 2  domain pair is located between the Cε3 and Cε4 domains from each chain and is no longer so closely associated with the Cε3 domain from chain A. 
     In a recent molecular dynamics simulation exploring unbending of IgE-Fc to an extended structure, it was found that while the acutely bent conformation observed in the crystal structure of free IgE-Fc occupied the lowest energy basin, another distinct and well defined energy basin, corresponding to partially bent IgE-Fc conformations, was observed 16 . The partially bent conformation adopted by IgE-Fc in the omalizumab Fab3/IgE-Fc complex occupies this particular energy basin ( FIG. 6 ). 
     The Cε3 Domains Adopt a Markedly Open Conformation in the Omalizumab Fab3/IgE-Fc Complex 
     In crystal structures of IgE-Fc and the Fcε3-4 sub-fragment, the Cε3 domains adopt a range of different orientations 7,8,10,11,13,14,16,24,29,  a property associated with allosteric regulation of IgE binding to its two principal receptors, FcεRI and CD23 8,11,12,14 . Both the distance between the Cε3 domains, and their positions with respect to the Cε4 domains, has been used to describe the variety of conformations observed for the Fcε3-4 region 29  (a full description for these measurements is provided later in this example). In the omalizumab Fab3/IgE-Fc complex, the Cε3 domains are positioned further away from one another, and from the Cε4 domains, than in any other crystal structure containing IgE-Fc or Fcε3-4, and thus adopt the most open conformation observed thus far ( FIG. 5 ); this conformation is significantly more open than the conformation for FcεRI-bound IgE-Fc ( FIG. 7 ). 
     Effect of Omalizumab Fab3 on FcεRI and CD23 Receptor Binding 
     Omalizumab inhibits not only the interaction between IgE-Fc and FcεRI, but also the interaction between IgE-Fc and CD23 30 . Consistent with the latter, comparison of the omalizumab Fab3/IgE-Fc and CD23/Fcε3-4 complexes 11  reveals clashes between omalizumab Fab3 and CD23 at both sites of CD23 engagement on Fcε3-4. Furthermore, Cε3 domain residues Arg376, Ser378 and Lys380 are involved in both omalizumab Fab3 and CD23 binding 11,31.    
     In contrast to CD23 binding to IgE, FcεRIα binds across both Cε3 domains. However, in the omalizumab Fab3/IgE-Fc complex, the Cε3 domains adopt a conformation that is too open to allow simultaneous engagement of both chains ( FIG. 8 ). Furthermore, if the omalizumab Fab3/IgE-Fc and sFcεRIα/IgE-Fc complexes are superimposed on each of the Cε3 domains in turn, potential steric clashes occur in each case. If superimposed on the Cε3 domain of chain A, omalizumab Fab3 Fab 2  would clash with the (Cε2) 2  domain pair in the FcεRIα complex, and the Cε2-Cε3 linker from the omalizumab Fab3 complex would potentially clash with FcεRIα. If superimposed on the Cε3 domain of chain B, there would be a potential clash between both omalizumab Fab3 Fab 1  and the Cε2-Cε3 linker (of the omalizumab Fab3 complex) with FcεRIα—although the binding sites for Fab 1  and FcεRIα do not actually overlap. 
     However, omalizumab Fab3 CDRL1 residues are positioned immediately adjacent to the FcεRIα-binding Cε3 domain FG loop. This loop, in chain B, contributes to a hydrophobic “proline sandwich” interaction, in which Pro426 in Cε3 packs between two tryptophan residues of FcεRIα. Asp32 (CDRL1) contacts Thr421, Gly33 (CDRL1) contacts Pro426, Arg427 and Ala428, and Asp34 (CDRL1) contacts Arg427 and Ala428. These interactions alter the position of the Cε3 domain FG loop and would further compromise the binding of IgE to FcεRI. 
     Recently, binding of omalizumab to FcεRIα-bound IgE has been reported 21,32 , although it is difficult to see how omalizumab might be able to engage FcεRI-bound IgE based on the static crystal structures of IgE-Fc in complex with sFcεRIα 8  and omalizumab Fab3. We therefore studied the binding of omalizumab Fab3 to IgE-Fc, and characterized the interaction between omalizumab Fab3 and the IgE-Fc/FcεRI complex. Our results provide insights into the mechanism of action of omalizumab. 
     Interaction of Omalizumab Fab3 with IgE-Fc in Solution 
     We characterized the IgE-Fc/omalizumab Fab3 interaction in two different ways, either by directly immobilizing omalizumab Fab3 on a surface and binding IgE-Fc, or binding omalizumab Fab3 to a His-tagged captured IgE-Fc on an SPR sensor surface. A C-terminally His-tagged IgE-Fc construct was captured using an anti-His-tag antibody (GE Healthcare), and the binding characteristics of omalizumab Fab3, intact omalizumab and omalizumab Fab were compared. Not surprisingly, in competition binding experiments, all three molecules competed for the same binding sites and showed broadly similar binding affinities (data not shown). The omalizumab Fab3 construct demonstrates slightly higher affinity compared with omalizumab Fab3 and intact omalizumab ( FIG. 8A-C ). Consistent with the crystal structure, two omalizumab Fab3 molecules bind to IgE-Fc: the binding is clearly biphasic with a high-affinity (˜1 nM) interaction observed at low ligand concentrations, and a second (weaker) binding site (˜30 nM) observed at higher concentrations ( FIG. 9A ). 
     A sandwich SPR experiment allowed the two IgE-Fc/omalizumab Fab3 binding sites to be characterized separately. Using this approach, omalizumab Fab3 was covalently immobilized on a sensor surface, and IgE-Fc was flowed over this surface. At low concentrations, under these conditions, the high-affinity site dominates the interaction and the binding curves can be described by monophasic interaction kinetics (K D ˜1 nM, k on ˜1.2×10 6  M −1  s −1 , k off ˜8×10 −4  s −1 ). This 1:1 IgE-Fc/omalizumab Fab3 complex, captured on the SPR biosensor surface, could then be used to measure the binding of the second omalizumab Fab3 molecule, the binding of which is significantly weaker (K D ˜30 nM, k on ˜2×10 5  M −1  s −1 , k off ˜6×10 −3  s −1 ) than the first ( FIG. 9B ). 
     Competition Between the Omalizumab Fab3 and FcεRIα Binding Sites and the Formation of an Omalizumab Fab3/IgE-Fc/FcεRIα Complex 
     We next investigated the capacity of omalizumab Fab3 to affect the interaction between IgE-Fc and FcεRIα. In solution competition binding experiments, increasing concentrations of omalizumab Fab3 inhibited binding of IgE-Fc to FcεRIα ( FIG. 8D ). Mechanistically, omalizumab Fab3 affects both the number of available binding sites (B max ) and the apparent K D  of the IgE-Fc/FcεRIα interaction; this is characteristic of a mixed inhibition mechanism 33 . Reduction in B max  values is indicative of an allosteric inhibitory process, and a decrease in the apparent affinity of the interaction is most commonly associated with direct competition for a shared binding site (i.e., orthosteric inhibition) but can also be seen for some allosteric inhibitors. Considering the binding sites observed in the crystal structure, it is likely that omalizumab Fab3 inhibits IgE-Fc binding to FcεRI using both orthosteric and allosteric mechanisms. 
     Competition between the omalizumab and FcεRIα binding sites has been described in many publications but has always been interpreted as direct competition for an identical (or overlapping) binding site. This interpretation has often been used to explain why omalizumab cannot bind to IgE-FcεRI complexes on cells. However, we observed that omalizumab Fab3 could bind with high affinity to IgE-Fc that was pre-bound to FcεRIα ( FIG. 9C , inset). The existence of an omalizumab/IgE-Fc/FcεRI complex has been implied by other studies 21,32 , but this complex has not before been experimentally characterized. The data indicate that while the binding of IgE-Fc to FcεRIα did not significantly change the affinity of omalizumab Fab3 for IgE-Fc, it did markedly change the number of available binding sites for omalizumab Fab3 in the population of FcεRIα-bound IgE-Fc molecules. We compared the K D  and B max  binding values for an IgE-Fc molecule captured via an anti-His-tag antibody with one captured by sFcεRIα, and found that FcεRIα-bound IgE-Fc had less than 10% of the omalizumab Fab3 binding sites compared to the His-tag captured IgE-Fc, which, as expected, showed binding levels consistent with 2:1 stoichiometry ( FIG. 9C ). So it is not, as has generally been assumed, that omalizumab does not bind to mast cell-bound IgE because the FcεRIα and omalizumab binding sites overlap. Instead, FcεRIα acts on IgE-Fc allosterically, changing a dynamic equilibrium of different IgE-Fc confonnations 16 , resulting in a substantially reduced number of omalizumab binding sites in a population of FcεRIα-bound IgE-Fc molecules. 
     Mechanism of Omalizumab Fab3-Mediated Accelerated Dissociation of the IgE-Fc/FcεRIα Complex 
     Kim et al. 20  reported that DARPin E2_79 could accelerate the disassembly of preformed complexes of IgE-FcεRI. Following up on this observation, Eggel et al. 21  later showed that omalizumab could also promote dissociation of IgE from FcεRI. Similar to these observations, we found that when omalizumab Fab3 bound to the IgE-Fc/FcεRIα complex, it could accelerate the dissociation of IgE-Fc from FcεRIα ( FIG. 9D ), and that omalizumab Fab3 did this more efficiently than omalizumab Fab3, and much more efficiently than intact omalizumab ( FIG. 10E ). One Fab engages the IgE-Fc/FcεRIα complex, but does not accelerate the dissociation of IgE-Fc from FcεRIα. Strikingly, it appears that accelerated dissociation occurs only after occupancy of the second binding site (i.e. the low-affinity site). The (omalizumab Fab3) 2 /IgE-Fc/FcεRIα tetramolecular complex must alter the energy landscape of IgE-Fc in such a way to markedly reduce the energy barrier for IgE-Fc/FcεRIα dissociation, resulting in a rapid dissociation of this otherwise very stable complex. 
     Details of the Interaction Between the Omalizumab Fab3 Light Chain and the Cε2 Domain 
     In the omalizumab Fab3/IgE-Fc complex, one Cε2 domain forms a minor interaction of approximately 260 Å 2  (compared with an average interaction area of ˜715 Å 2  between omalizumab Fab3 and the Cε3 domain) with two of the mutated residues (Ser81Arg and Gln83Arg). There is no contact between Pro158 and IgE-Fc. 
     The Arg81 side chain, from the Fab 2  light chain (one of the mutated residues in omalizumab Fab3; (Seq. ID No 125, PDB numbering)), packs against Val277 and Asp278 from the Cε2 domain from chain B (Seq. ID No. 108). Ser80 (omalizumab Fab3) packs against Asp278, Leu279 and Thr281 (Cε2 domain), while Ser64 (omalizumab Fab3) packs against Asp276 and Asp278. Ser64 and Ser80 are identical in omalizumab and omalizumab Fab3. 
     In the omalizumab Fab3/IgE-Fc complex, Arg83 (one of the mutated residues in omalizumab Fab3) does not apparently contact the Cε2 domain, due to disorder in the Asp278 (Cε2 domain) side chain. However, if the Asp278 side chain were ordered, a hydrogen bond or salt bridge could potentially form between Arg83 and Asp278. 
     Crystallographically-Determined Contacts Between Omalizumab Fab3/IgE-Fc Complex 
     Contacts between antibody and antigen within 4 Å in a crystal structure are typically indicative of the epitope/paratope interface. 
     The IgE-Fc residues within 4 Å of the omalizumab Fab3 heavy and light chain CDRs define the following epitope: 
     T 373, W 374, S 375, R 376, A 377, 3 S 78, G 379, P 381, Q 417, C418, R 419, P 426, R 427, A 428 (on chain A). 
     Furthermore, the IgE-Fc residues within 4 Å of the omalizumab Fab3 light chain FR1 and FR3 residues extend the epitope to: 
     D 278, T 281 (on chain B—Cε2 domain) [with contacts to R18, S64, S80 and R81 of the antibody—and further R83 as revealed during the molecular dynamics simulation described in Example 5]. 
     Contacts between antibody and antigen within 5 Å in a crystal structure also are informative in defining the antibody/antigen interface. 
     The additional IgE-Fc residues within 5 Å of the omalizumab Fab3 heavy and light chain CDRs are: K380, M430 (on chain A). 
     Furthermore, the additional IgE-Fc residues within 5 Å of the omalizumab Fab3 light chain FR1 and FR3 residues are: D276, V277, L279, S280, A282 (on chain B—Cε2 domain) [with further contacts to G16 of FR1 and R65 of FR3]. 
     Analysis of Cε3 Domain Orientation in the Omalizumab Fab3/IgE-Fc Complex 
     In one method to analyses the position of the Cε3 domains with respect to the Cε4 domains, the interatomic distance between the Asn394 Cα atom from the Cε3 domain from one chain, and the Lys497 Cα atom from the Cε4 domain of the other chain has been used to describe the “openness” of the Cε3 domains 29 . The interatomic distance between the Val336 Cα has been used to describe the “swing”, or how close the Cε3 domains are to one another 29 . 
     For FcεRI-bound IgE-Fc, and FcεRI-bound Fcε3-4, in which the Cε3 domains adopt an open conformation, “openness” values range from 23.5-28.4 Å, while the “swing” values are an average of 23.3 Å 8,10 . Corresponding values for the omalizumab Fab3/IgE-Fc complex are an average of 29.5 for the “openness” and 29.4 Å for the “swing”. In the omalizumab Fab3 complex, the Cε3 domains adopt the most open conformation (furthest apart from one another), described thus far. 
     Discussion 
     We report the structure, at 3.7 Å resolution, of the complex between IgE-Fc and a Fab fragment derived from the therapeutic anti-IgE antibody omalizumab; we call this Fab fragment, which contains three point mutations in framework regions distal to the antigen-binding site, omalizumab Fab3. The structure reveals two omalizumab Fab3 molecules in complex with IgE-Fc, one bound to each of the two Cε3 domains (but only one of the Fabs bound to a Cε2 domain), and provides an explanation for the ability of omalizumab to inhibit the binding of IgE to both FcεRI and CD23. IgE-Fc is also found to adopt a partially bent conformation in the omalizumab Fab3 complex, consistent with our earlier study using a FRET-labelled IgE-Fc, which indicated a slight unbending relative to free IgE-Fc 9 . 
     IgE-Fc is predominantly bent in solution 5,6,9,25,26,27,28 , and in the crystal structure of free IgE-Fc, the (Cε2) 2  domain pair is folded back against the Cε3 and Cε4 domains 7,8 . Recently, our understanding of the conformational flexibility of IgE-Fc was profoundly enhanced when we solved the structure of a fully extended conformation, captured in a complex with an anti-IgE-Fc Fab (aεFab) 16 . A molecular dynamics simulation, exploring IgE-Fc unbending from the acutely bent to the extended conformation, revealed energy basins corresponding to partially bent conformations ( FIG. 6 ). The omalizumab Fab3/IgE-Fc complex reported here, in which the bend between the (Cε2) 2  domain pair and the Fcε3-4 domains is ˜90°, corresponds to a distinct energy basin in this simulation 16 . Intriguingly, the location of the omalizumab Fab3 binding site would not preclude further unbending to the fully extended conformation and it is therefore possible that IgE-Fc can undergo substantial changes in conformation even when in complex with omalizumab. 
     In addition to the bending of the (Cε2) 2  domain pair relative to the Cε3 and Cε4 domains, the various IgE-Fc, Fcε3-4 and receptor complex structures have demonstrated that the Cε3 domains can adopt a range of relative orientations, from closed to open 7,8,10,11,13,14,16,24,29 . Opening and closing of the Cε3 domains contributes to the allosteric regulation of receptor binding in IgE-Fc 11,12 : in the CD23 complex they are relatively closed 11,13,14 , whereas in the FcεRI complex they are more open 8,10 . Comparison of the structures of the CD23/Fcε3-4 and omalizumab Fab3/IgE-Fc complexes shows that the CD23 and omalizumab sites overlap, and competition binding experiments indicate that inhibition of IgE binding to CD23 by omalizumab is straightforwardly orthosteric. 
     However, inhibition of FcεRI binding is more complicated. In the omalizumab Fab3 complex, the Cε3 domains adopt a more open conformation than seen in any previous structure, so much so that the two sub-sites of interaction between IgE-Fc and FcεRI, one involving each Cε3 domain, cannot engage simultaneously. Another contribution to this inhibition may result from the proximal location of the omalizumab Fab3 (Fab) molecule to the receptor-binding FG loop in Cε3, which may directly affect the conformation of contact residues for FcεRIα. Finally, even though the omalizumab Fab3 and FcεRIα binding epitopes on IgE-Fc do not strictly overlap, there is the possibility of steric clashes if the two were bound simultaneously. Thus, the crystal structure suggests that omalizumab&#39;s mechanism of inhibition is principally allosteric but with a potential orthosteric component. 
     SPR studies enabled us to assess the kinetics and affinities for the two omalizumab Fab3 binding sites. The two affinities differ markedly, with K D  values of ˜1 nM and ˜30 nM, the former associated with a faster association rate constant (k on ˜2×10 6  M −1  s −1  compared with ˜2×10 5  M −1  s −1 ) and a slightly slower dissociation rate constant (k off ˜2×10 −3  s −1  compared with ˜6×10 −3  s −1 ). It might be speculated that the higher affinity interaction corresponds to the binding of Fab 1 , which would have unimpeded access to a bent IgE-Fc molecule, while the lower affinity and slower on-rate corresponds to Fab 2 , but we cannot be definitive about this. 
     Further SPR experiments to investigate the mechanism of the inhibition of IgE-Fc binding to FcεRIα by omalizumab Fab3 revealed a reduction in the number of available sites for omalizumab Fab3 on IgE-Fc (reduced B max ) when in complex with FcεRIα. The inhibition of IgE binding to FcεRI by omalizumab has frequently been interpreted in terms of direct competition for overlapping sites, but there have been reports that indicate that omalizumab can bind to receptor-bound IgE 21,32 . We have here demonstrated directly the ability of omalizumab Fab3 to bind to IgE-Fc when it is already bound to FcεRIα to form a trimolecular complex. The effect of the pre-binding of IgE-Fc to FcεRIα is to reduce the number of omalizumab Fab3 binding sites on IgE-Fc to less than 10% of those available in free IgE-Fc; this effect can only be due to allosteric modulation. 
     The nature of the interaction of omalizumab Fab3 with the IgE-Fc/FcεRI complex provides insights into the mechanism of accelerated dissociation. This phenomenon was first reported for a DARPin and subsequently for omalizumab 20,21 , the latter at substantially greater concentrations than those used therapeutically 22 , and is now shown here for omalizumab Fab fragments. We further demonstrate that the dissociation occurs only after first binding of the second (lower affinity) omalizumab Fab3 molecule. Stated another way, a tetramolecular complex—(omalizumab Fab3) 2 /IgE-Fc/FcεRIα—must be formed for significant accelerated dissociation to occur. 
     Based on our observations with omalizumab Fab3, IgE-Fc and sFcεRIα, we envisage the following mechanism occurring for omalizumab, IgE and FcεRI: IgE binds to FcεRI and, under these conditions, a small population of these bound IgE molecules adopt a conformation to which omalizumab molecules can bind; when a second omalizumab molecule binds to form the tetrameric complex, the energy landscape of IgE is changed such that the interaction with FcεRI is destabilized, and a rapid dissociation of IgE from FcεRI occurs. Key to understanding this mechanism is an appreciation of the complexity of the energy landscape for IgE, and the different conformational states that exist in dynamic equilibrium. 
     The inhibitory activities of omalizumab appear to take advantage of the intrinsic flexibility of IgE and, at least for the process of accelerated dissociation, the dynamics of the IgE/FcεRI complex. IgE has a number of unusual structural characteristics compared to other antibody isotypes, including the presence of the Cε2 domains and the uniquely conformationally dynamic, molten globule-like character of the Cε3 domains 34 . Together, these properties create an allosteric communication pathway that prevents simultaneous engagement of CD23 and FcεRI; this is essential to avoid allergen-independent mast cell activation by crosslinking of FcεRI-bound IgE by the trimeric CD23 molecule 12 . Other functional advantages associated with the dynamics of IgE have been proposed for the membrane-bound IgE B cell receptor 16 . The observation that omalizumab does not utilize the expected orthosteric mechanism for inhibition of the IgE/FcεRI interaction indicates that it also exploits the unusual dynamic properties of IgE, both in its capacity as a blocking antibody and its ability to avoid crosslinking of mast cell-bound IgE. Finally, omalizumab can actively dissociate IgE from FcεRI, albeit at concentrations higher than used therapeutically 21 , by employing allostery and the intrinsic flexibility of IgE, present even when in complex with its receptors. 
     Methods 
     Cloning, protein expression and purification. Omalizumab human IgG 1  Fab and omalizumab Fab3 were cloned, expressed and purified as described in 16 . IgE-Fc was produced as described previously 35 . IgE-Fc was according to Seq. ID No. 108 (V224-K547 according to Dorrington &amp; Bennich (1978) Immunol. Rev. 41:3-25, but with the following mutations inserted into the IgE-Fc to simplify the glycosylation pattern: N265Q &amp; N371Q). Omalizumab was purchased from Novartis Europharm Limited. The 2:1 omalizumab Fab3/IgE-Fc complex was purified by size exclusion chromatography, eluted into 25 mM Tris-HCl pH7.5, 20 mM NaCl and 0.05% (w/v) NaN 3 , and concentrated to 23 mg/mL. 
     Surface Plasmon Resonance. SPR experiments were carried out on a Biacore T200 instrument (GE Healthcare). Specific surfaces were prepared either by covalently coupling proteins using the amine coupling protocol (GE Healthcare), with coupling densities &lt;300 resonance units, or capturing His-tagged proteins using an anti-His sensor surface. For capturing His-tagged ligands, an anti-His-tag monoclonal antibody was employed and immobilized according to manufacturer&#39;s instructions (Biacore His Capture Kit, GE Healthcare). In binding experiments, association times of 180-240 s were typically used, and dissociation components were monitored for at least 500 s. Injections were performed at a flow rate of 25 μL min −1 , in a running buffer of 20 mM HEPES pH 7.4, 150 mM NaCl, and 0.005% (v/v) surfactant P-20 (GE Healthcare). Most experimental measurements were performed at 25° C.; some of the sandwich binding experiments were done at 5° C. in order to minimize the accelerated dissociation phenomenon. Standard double referencing data subtraction methods were used 36  and kinetic fits were performed using Origin software (OriginLab). 
     TR-FRET. IgE-Fc was labeled with donor fluorophore by reacting 4 mg/mL protein in 100 mM sodium bicarbonate, 50 mM NaCl, pH 9.3, with a 5-fold molar excess of terbium chelate isothiocyanate (Invitrogen). After 3 hr incubation at room temperature with agitation, excess unreacted fluorophore was removed by dialyzing into PBS (20 mM phosphate buffer saline, 150 mM NaCl, pH 7.4). sFcεRIα-IgG4-Fc fusion protein (α-γ) 37  was labeled with acceptor fluorophore by reacting 3 mg/ml protein with a 2.5-fold molar excess of Alexa Fluor 647 succinimidyl ester (Invitrogen) for 1 hr at room temperature. Excess fluorophore was removed by dialyzing into PBS. 
     TR-FRET inhibition assays were performed by competing 1 nM terbium labeled IgE-Fc and 0-20 nM of Alexa Fluor 647 labeled sFcεRIα-IgG 4 -Fc with a range of concentrations of omalizumab Fab3. Assays were conducted in 384 well hi-base, white plates (Greiner BioOne) using Lanthascreen buffer (Invitrogen) as a diluent. The plate was left to incubate overnight at room temperature and read by an Artemis plate reader (Berthold Technologies). TR-FRET ratios were then calculated for each well as the emission of acceptor at 665 nm divided by the emission of donor at 620 nm multiplied by 10,000. 
     Crystallization. Crystals with a rectangular morphology, up to 400 μm in length, were grown at 18° C. using the sitting drop vapour diffusion method. The reservoir contained 50 μL 4% (w/v) PEG 8000 and 0.03M sodium fluoride, and the drop contained 100 nL protein and 300 nL reservoir. Despite extensive efforts at optimisation, the diffraction quality of the crystals could not be further improved beyond that used for this study. Crystals typically started to grow after a few days, and often dissolved in their drops, but could be stabilized in 4M TMAO (trimethylamine N-oxide), which was successfully used as a cryoprotectant. 
     Data collection and processing. Data were collected at beamlines 102 and 103 at the Diamond Light Source (Harwell, UK). Integration was performed using XDS 38  as implemented in the xia2 package 39 . The crystals diffracted anisotropically, and data from multiple crystals were merged. The data were scaled to 3.7 Å resolution with AIMLESS from the CCP4 suite 40,41  and then truncated to resolution limits of 3.7 Å (a*), 3.9 Å (b*) and 4.2 Å (c*) using the UCLA Diffraction Anisotropy Server 42 . Calculation of the Matthews coefficient indicated a solvent content of ˜62%, for a single 2:1 omalizumab Fab3/IgE-Fc complex (molecular mass of ˜170 kDa) in the asymmetric unit. 
     Structure determination, model building and refinement. The structure was solved by molecular replacement with PHASER 43  and MOLREP 44  from the CCP4 suite 40  using protein atoms from PDB entry 2wqr 8  and a 1.91 resolution omalizumab Fab structure (unpublished results) as search models. Refinement was initially performed with REFMAC 45  and later with PHENIX 46 , and alternated with manual model building in Coot 47 . The quality of the model was assessed with MolProbity 48 , POLYGON 49 , and other validation tools within the PHENIX graphical interface 50 . Data processing and refinement statistics are presented in Table 1. A region of the electron density map is shown in  FIG. 11 . Interfaces were analysed with PISA 51 , and figures were prepared with PyMOL 52 . 
     
       
         
           
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Data processing and refinement statistics 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Data processing 
                   
               
               
                 Space group 
                 I 2 1  2 1  2 1   
               
               
                 Unit cell dimensions (Å) 
                 a = 76.64, b = 231.19, c = 247.12 
               
            
           
           
               
               
               
            
               
                 Resolution (Å): overall (outer shell) 
                 115.59-3.70 
                 (4.10-3.70) 
               
               
                 Completeness (%) a   
                 99.9 
                 (99.9) 
               
               
                 Multiplicity a   
                 38.0 
                 (38.4) 
               
               
                 Mean ((I)/σ(I)) a   
                 17.9 
                 (1.9) 
               
               
                 R pim  (%) a   
                 2.6 
                 (56.3) 
               
               
                 Refinement b   
               
            
           
           
               
               
            
               
                 R work /R free  (%) c   
                 25.88/30.92 
               
               
                 No. of reflections 
                 20 087 
               
            
           
           
               
               
               
            
               
                 RMSD 
                   
                   
               
            
           
           
               
               
            
               
                 Bond lengths (Å) 
                 0.002 
               
               
                 Bond angles (°) 
                 0.451 
               
               
                 Coordinate error (Å) 
                 0.60 
               
               
                 Ave. B-factor (Å 2 ) 
                 171.2 
               
            
           
           
               
               
               
            
               
                 Ramachandran plot 
                   
                   
               
            
           
           
               
               
            
               
                 Favoured (%) 
                 95.81 
               
               
                 Allowed (%) 
                 100.00 
               
               
                   
               
               
                   a Values in parentheses are for the highest resolution shell 
               
               
                   b Refinement was performed with data truncated to resolution limits of 3.7 Å (a*), 3.9 Å (b*) and 4.2 Å (c*) 
               
               
                   c R free  set comprises 5% of reflections 
               
            
           
         
       
     
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     Example 2: Measuring of Accelerated Dissociation of IgE-Fc from Immobilised sFcεRIα by Biacore 
     The Biacore technology measures the interaction between biomolecules without the requirement for labelling. One of the interactants, termed the ligand, is either immobilised directly on or captured to the sensor surface while the other, termed the analyte, flows in solution over the captured surface. The sensor detects the change in mass at the sensor surface as the analyte binds to the ligand and when the analyte dissociates from the ligand. These correspond to both the association and dissociation processes. In the accelerated dissociation assay sFcεRIα is the ligand and is immobilized to the sensor surface. IgE-Fc is the analyte and is captured by the sFcεRIα. The dissociation of IgE-Fc from sFcεRIα is monitored either with buffer flowing over the sensor surface or with a solution of IgE binding partner flowing over the sensor surface. Details of the method are as follows: 
     Instrument: Biacore 3000, GE Healthcare AB, Uppsala, Sweden 
     Sensor chip: CMS. Catalogue number BR100399 
     BIAnormalising solution: 70% (w/w) glycerol. Part of the BIAmaintenance Kit. Catalogue number BR100651. The BIAmaintenance kit was stored at 4° C. 
     Amine Coupling Kit: Catalogue number BR100633. Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) made up to 75 mg/mL in distilled water and stored in 200 uL aliquots at −70° C. N-Hydroxysuccinimide (NHS) made up to 11.5 mg/mL in distilled water and stored in 200 uL aliquots at −70° C. Ethanolamine hydrochloride-NaOH pH 8.5 stored at 4° C. 
     Reagent for the oxidisation of sFcεRIα. Carbohydrazide (SigmaAldrich, catalogue number C11006) made up to 5 mM in distilled water. Sodium cyanoborohydride (SigmaAldrich, catalogue number 156159) made up to 100 mM in sodium acetate, (BDH, cat. S1104-500GM) 100 mM pH=4. Sodium m-periodate (SigmaAldrich, catalogue number S-1878) made up to 50 mM in sodium acetate (BDH, cat. S1104-500GM) 100 mM, pH=5.5. 
     sFcεRIα was diluted to 1 mg/ml in pH 5.5, 0.1M sodium acetate. Then 4 ul of Sodium periodate (50 mM), dilution 1/50) was added to 200 ul of 1 mg/ml of sFcεRIα solution. The mixture was left on ice for 20 min. Prior the immobilisation the solution of sFcεRIα was diluted to 1 ug/ml with 10 mM sodium acetate (GE Healthcare, Catalogue number BR100669), pH=4.0. 
     Buffers: Running buffer is HBS-EP (being 10 mM HEPES pH 7.4, 150 mM NaCl 3 mM EDTA, 0.05% Surfactant P20, reconstituted from 10× stock solution): Catalogue number BR100669. Immobilisation buffer is Acetate 4.0 (being 10 mM sodium acetate pH 4.0). Catalogue number BR100349. Buffer stored at 4° C. 
     Ligand: sFcεRIα, being the extracellular portion of the alpha chain of the human high affinity IgE receptor. Expressed as a recombinant protein in CHO cells and purified. 
     Analyte: IgE-Fc, being the Fc portion of human IgE, expressed as a recombinant protein in CHO cells and purified. Wild-type human IgE-Fc (Cε2-Cε4 domains with numbering V224-K547 according to Dorrington &amp; Bennich (1978) Immunol. Rev. 41:3-25) carrying a C225A mutation was used (Seq. ID No. 108). 
     Nomenclature of mutants:
         Omalizumab Fab 1: S81R, Q83R [S77R, Q79R according to Kabat numbering] (variable region light chain+Kappa constant region as Seq. ID No. 24 wherein S77 and S79 are replaced by Q; variable region heavy chain+CH1 constant region as SEQ ID NO: 5)   Omalizumab Fab 2: L158P [L154P according to Kabat numbering] (variable region light chain+Kappa constant region as Seq. ID No 113; variable region heavy chain+CH1 constant region as SEQ ID NO: 5)   Omalizumab Fab 3: S81R, Q83R, L158P [S77R, Q79R, L154P according to Kabat numbering] (variable region light chain Seq. ID No 121; variable region heavy chain+CH1 constant region as SEQ ID NO: 5)       

     IgE binding partners (1): full length Omalizumab (Novartis); recombinant Fab fragment of omalizumab expressed in CHO cells and purified. 
     IgE binding partners (2): recombinant Fab fragment of omalizumab, and mutations thereof, expressed in HEK-293 cells and assayed as culture supernatant. Culture supernatant was concentrated ten-fold prior to analysis. 
     Assay method (1): sFcεRIα was coupled to the sensor surface by aldehyde coupling to a level of ˜500 response units (RU). HBS-EP buffer was uses as the running buffer at a flow rate of 30 uL/min. IgE-Fc was diluted to 10 nM in HBS-EP and injected over the immobilised sFcεRIα for 290 seconds followed by 3 injections, each of 690 seconds duration, of running buffer or of IgE binding partner diluted in running buffer. Capture level of IgE-Fc was ˜90 RU. The surface was regenerated with two 60 second injections of 10 mM glycine-HCl, pH 2.5. The amount of dissociation of IgE-Fc from the immobilised sFcεRIα was calculated as a function of the initial binding amount and the rate of dissociation was calculated as the amount of loss of IgE-Fc from the immobilised sFcεRIα, normalised for the initial binding amount as a function of elapsed time. 
     Assay method (2): sFcεRIα was coupled to the sensor surface by aldehyde coupling to a level of ˜2000 response units (RU). HBS-EP buffer was uses as the running buffer at a flow rate of 30 uL/min. IgE-Fc was diluted to 10 nM in HBS-EP and injected over the immobilised sFcεRIα for 180 seconds followed by 1 injection of 180 seconds duration, of running buffer or of IgE binding partner diluted in running buffer. Capture level of IgE-Fc was ˜275 RU. The surface was regenerated with two 60 second injections of 10 mM glycine-HCl, pH 2.5. The amount of dissociation of IgE-Fc from the immobilised sFcεRIα was calculated as a function of the initial binding amount and the rate of dissociation was calculated as the amount of loss of IgE-Fc from the immobilised sFcεRIα, normalised for the initial binding amount as a function of elapsed time. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 calculation of the amount of dissociation of IgE-Fc from immobilised sFcεRIα. 
               
               
                 Initial binding of IgE to sFcεRIα is normalised to 100% and dissociation calculated 
               
               
                 relative to that. The apparent dissociation rate is based on an assumed single rate process. 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                 Amount of 
                 Amount of 
                 Amount of 
                 Apparent 
               
               
                   
                   
                 IgE-Fc 
                 IgE-Fc 
                 IgE-Fc 
                 dissociation 
               
               
                   
                   
                 remaining 
                 remaining 
                 remaining 
                 rate based 
               
               
                   
                   
                 bound to 
                 bound to 
                 bound to 
                 on 3 rd   
               
               
                   
                 Capture 
                 sFcERIa 
                 sFcERIa 
                 sFcERIa 
                 Injection of 
               
               
                   
                 level of 
                 after 1st 
                 after 2nd 
                 after 3rd 
                 IgE binding 
               
               
                   
                 IgE-Fc 
                 injection 
                 injection 
                 injection 
                 partner 
               
               
                 IgE binding partner 
                 (%) 
                 (%) 
                 (%) 
                 (%) 
                 (1/s) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Omalizumab 300 ug/mL 
                 100.0 
                 79.3 
                 71.5 
                 69.9 
                 1.46E−04 
               
               
                 buffer 
                 100.0 
                 87.6 
                 81.9 
                 77.9 
                 1.02E−04 
               
               
                 Omalizumab Fab1 100 ug/mL 
                 100.0 
                 61.3 
                 51.0 
                 47.3 
                 3.06E−04 
               
               
                 Omalizumab Fab2 100 ug/mL 
                 100.0 
                 79.4 
                 71.5 
                 69.5 
                 1.48E−04 
               
               
                 Omalizumab Fab3 100 ug/mL 
                 100.0 
                 45.3 
                 34.6 
                 30.8 
                 4.80E−04 
               
               
                   
               
            
           
         
       
     
     Additional mutagenesis was performed to determine if further mutations that accelerated the dissociation of IgE-Fc from sFcεRIα, outside of those described in Fab3, could be identified. This led to the mutation S64M (with reference to Seq. ID No. 20) which, in the context of Fab3 was able to further increase the dissociation of IgE from sFcεRIα. These data are described in  FIG. 13  and Table 3. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 calculation of the amount of dissociation of IgE-Fc from immobilised sFcεRIα. Initial 
               
               
                 binding of IgE to sFcεRIα is normalised to 100% and dissociation 
               
               
                 calculated relative to that. The apparent dissociation rate is based on an 
               
               
                 assumed single rate process. Mutant S64M: light chain Seq. ID No 39; omalizumab 
               
               
                 Fab WT: light chain Seq. ID No 20; omalizumab Fab3: light chain Seq. ID No 
               
               
                 125. For all three, heavy chain SEQ ID NO: 5. 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                 RU of 
                 RU of 
                   
                 apparent 
               
               
                   
                   
                 IgE-Fc 
                 IgE-Fc after 
                 % 
                 dissociatior 
               
               
                 Sample 
                 Identity 
                 captured 
                 dissociation 
                 dissn 
                 rate (1/s) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 cells only control 
                 control supernatant 
                 256.19 
                 215.2 
                 16.00 
                 7.03E−04 
               
               
                 mutation S64M 
                 omalizumab Fab (S64M 
                 278.59 
                 94.66 
                 66.02 
                 4.30E−03 
               
               
                   
                 S81R, Q83R, L158P) 
               
               
                 omalizumab Fab WT 
                 omalizumab Fab WT 
                 265.49 
                 205.05 
                 22.77 
                 1.04E−03 
               
               
                 omalizumab Fab3 
                 omalizumab Fab (S81R, 
                 261.85 
                 163.32 
                 37.63 
                 1.90E−03 
               
               
                   
                 Q83R, L158P) 
               
               
                   
               
            
           
         
       
     
     Conclusion: 
     Taken together, these data demonstrate that a mutated form of omalizumab Fab can accelerate the dissociation of IgE from an immobilised form of the high affinity IgE receptor, FcεRI. The mutations in the light chain that enable this include, but are not necessarily limited to, S64M, S81R, Q83R and L158P with reference to SEQ ID NO: 24 and resulting in SEQ ID NO: 39. 
     Example 3: Measuring the Accelerated Dissociation of IgE-Fc from FcεRI by Flow Cytometry 
     Instrument: FACSCanto II flow cytometer (Becton Dickinson) 
     Cell line: RBL-SX38 cells expressing human FcεRI were cultured in Minimal Essential Medium supplemented with 10% foetal bovine serum, 2 mM GlutaMAX and 500 μg/mL Geneticin (Life Technologies). At the time of assay, cells were washed in PBS and incubated in Accutase until detached, then resuspended at 1×10 6  cells/mL in culture media. All subsequence incubation steps were performed in culture media. 
     Assay method: RBL-SX38 cells at 1×10 6  cells/mL were incubated with 5 nM Alexa-488 labelled IgE-Fc for 1 hour at 37° C. Cells were washed twice in culture media to remove unbound IgE-488 then suspended at 1×10 6  cells/mL in culture media or 100 μg/mL IgE-binding agents diluted in culture media. Cells were then incubated at 37° C. with constant rotation. At each time point, 0.5×10 5  cells were removed, washed in ice-cold PBS and then fixed by resuspending in 1% paraformaldehyde in PBS for 16 hours at 4° C. The amount of cell-bound Alexa-488 fluorescence was determined using a FACSCanto II flow cytometer. 
     Flow cytometry: fixed cells were washed twice in FACS buffer (0.1% w/v BSA, 0.01% w/v NaN 3  in PBS, pH 7.4) and resuspended in 200 μL FACS buffer. Flow cytometry was performed on a FACSCanto II cytometer using standard methods and the geometric mean fluorescence intensity of Alexa-488 bound to intact cells was calculated using FlowJo software. The dissociation rate of Alexa-488 labelled IgE-Fc was calculated as the change in geometric mean fluorescence intensity as a function of time as a result of either incubating the cells in culture media, excess unlabelled IgE-Fc or in the presence of IgE binding agents. 
     Nomenclature of mutants:
         Omalizumab Fab 1: S81R, Q83R [S77R, Q79R according to Kabat numbering] (variable region light chain+Kappa constant region as Seq. ID No. 24 wherein S77 and S79 are replaced by Q; variable region heavy chain+CH1 constant region as SEQ ID NO: 5)   Omalizumab Fab 2: L158P [L154P according to Kabat numbering] (variable region light chain+Kappa constant region as Seq. ID No 113; variable region heavy chain+CH1 constant region as SEQ ID NO: 5)   Omalizumab Fab 3: S81R, Q83R, L158P [S77R, Q79R, L154P according to Kabat numbering] (variable region light chain+Kappa constant region as Seq. ID No 121; variable region heavy chain+CH1 constant region as SEQ ID NO: 5).       

     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 calculated dissociation rate of Alexa488 labelled IgE-Fc from 
               
               
                 the surface of RBL-SX38 cells. Apparent dissociation rate determined 
               
               
                 from a linear regression fit of the plot of −ln(R1/R0) as 
               
               
                 a function of time for all available time points. 
               
            
           
           
               
               
               
            
               
                   
                 Concentration 
                 Dissociation rate (×10−5) 
               
               
                 IgE binding partner 
                 (ug/mL) 
                 1/s 
               
               
                   
               
            
           
           
               
               
               
            
               
                 UT 
                   
                 1.38 
               
               
                 Omalizumab 
                 100 
                 1.77 
               
               
                 Omalizumab 
                 31 
                 1.56 
               
               
                 Omalizumab 
                 10 
                 1.32 
               
               
                 Omalizumab 
                 3.1 
                 1.45 
               
               
                 Omalizumab 
                 1 
                 1.36 
               
               
                 Omalizumab Fab 
                 100 
                 2.09 
               
               
                 Omalizumab Fab 
                 31 
                 1.63 
               
               
                 Omalizumab Fab 
                 10 
                 1.35 
               
               
                 Omalizumab Fab 
                 3.1 
                 1.38 
               
               
                 Omalizumab Fab 
                 1 
                 1.59 
               
               
                 Omalizumab Fab1 
                 100 
                 4.14 
               
               
                 Omalizumab Fab1 
                 31 
                 2.6 
               
               
                 Omalizumab Fab1 
                 10 
                 1.93 
               
               
                 Omalizumab Fab1 
                 3.1 
                 1.61 
               
               
                 Omalizumab Fab1 
                 1 
                 1.46 
               
               
                 Omalizumab Fab2 
                 100 
                 2.11 
               
               
                 Omalizumab Fab2 
                 31 
                 1.65 
               
               
                 Omalizumab Fab2 
                 10 
                 1.39 
               
               
                 Omalizumab Fab2 
                 3.1 
                 1.36 
               
               
                 Omalizumab Fab2 
                 1 
                 1.29 
               
               
                 Omalizumab Fab3 
                 100 
                 3.99 
               
               
                 Omalizumab Fab3 
                 31 
                 2.81 
               
               
                 Omalizumab Fab3 
                 10 
                 2.03 
               
               
                 Omalizumab Fab3 
                 3.1 
                 1.5 
               
               
                 Omalizumab Fab3 
                 1 
                 1.47 
               
               
                 Isotype control 
                 100 
                 1.3 
               
               
                 Isotype control 
                 31 
                 1.28 
               
               
                 Isotype control 
                 10 
                 1.35 
               
               
                 Isotype control 
                 3.1 
                 1.19 
               
               
                 Isotype control 
                 1 
                 1.25 
               
               
                 IgE Quench 
                   
                 1.24 
               
               
                   
               
            
           
         
       
     
     Conclusion: 
     Taken together, these data demonstrate that a mutated form of omalizumab Fab can accelerate the dissociation of IgE from the high affinity IgE receptor, FcεRI, expressed on the cell surface. The mutations in the light chain that enable this include, but are not necessarily limited to, S81R, Q83R and L158P. 
     Example 4: Demonstrating that Omalizumab Fab3 has Superior Efficacy to Wild Type Omalizumab Fab when Dosed Therapeutically in the 72 Hour PCA Model 
     A solution of 25 ug/mL of human anti DNP-IgE was prepared by adding 7.5 uL of 6.68 mg/mL stock to 1992.5 uL PBS. A 20 uL injection of this solution would give a 500 ng dose of IgE. Animals (hIgER Tg mice) were shaved on the flanks and then injected i.d on each flank at 2 pm on day 0.20 uL of PBS was injected into the left flank of each animal as a negative control. Anti DNP-IgE (20 uL) was injected into the right flank. A total of 40 mice were injected in total. Treatment with either wild type omalizumab Fab or omalizumab Fab3 (with S81R, Q83R, L158P mutations [S77R, Q79R, L154P according to Kabat numbering]) commenced 18 hours post IgE (8 am). Two groups of mice (n=8/gp) received 100 mg/kg s.c of either wild type omalizumab Fab or omalizumab Fab3. A further group of 8 mice received PBS s.c. Mice were dosed again as above 10 hrs later (6 pm). At this time point, 28 hrs post IgE, a further two groups (n=8/gp) were also dosed with either wild type omalizumab Fab or omalizumab Fab3 at 100 mg/kg sc. All groups were re-dosed at 8 am, 6 pm and again at 8 am on the final day of the experiment. 72 hours post i.d dosing (2 pm) all animals were injected i.v with 100 uL of 1 mg/mL DNP-HSA, 2.5% w/v Evans blue made up in 100 IU/ml of heparin. 1 hour later animals were killed by a schedule 1 method. The skin from the flanks around the i.d injection site was removed and a punch biopsy taken. Skin samples were placed into 700 uL formamide and digested overnight at 55° C. Following digestion 100 uL×2 fluid was removed from each sample and placed into a 96 well ELISA plate. Absorbance was then measured at 620 nm. 
     Conclusion: 
     These data demonstrate that a mutated form of omalizumab Fab that can accelerate the dissociation of IgE from the high affinity IgE receptor, FcεRI, is also able to reduce passive cutaneous anaphylaxis (as shown by inhibition of leakage of Evans blue dye from the reaction site) in a statistically significant manner when compared with wild type omalizumab Fab. The mutations in the light chain that enable this include, but are not necessarily limited to, S81R, Q83R and L158P with reference to SEQ ID NO: 24 and resulting in SEQ ID NO: 39. 
     Example 5: Molecular Dynamics Simulation of OmalizumabFab3 in Complex with IgE Fc 
     Method: 
     The crystal structure of Omalizumab Fab3 in complex with IgE Fc region (see Example 1) was prepared in Molecular Operating Environment (MOE) 2014.0901 (1) by completing the missing side chains in some residues and missing loops between Cε2 and Cε3 domains prior to molecular dynamics (MD) simulation using Amber 14 (2). The complex structure was hydrogen-added and solvated using TIP3P explicit water model with 0.15M NaCl salt solute in a truncated octahedron box that extended 10 Å from any edge of the box to the protein atoms. The system was setup using Amber ff12SB and oligosaccharides GLYCAM_06 j-1 (3) forcefields and minimized by conjugate gradient algorithm for 50,000 steps with a 10.0 Å cutoff set for Coulombic and Van der Waals interactions and a grid-based neighbour list. Afterwards the system was gradually heated from 0 to 300 K in 125 ps at constant volume followed by 2.25 ns equilibration in the NPT ensemble with restraints on all solute heavy atoms (harmonic force constraint is 5.0). For electrostatics, we used fourth order PME with a cutoff of 8.0 Å for Coulombic interactions with default Fourier spacing and tolerance settings. The temperature was controlled with weak-coupling algorithm applied to the protein and solvent respectively with a time constant of 1.0 ps, and the pressure was controlled with an isotropic Berenson barostat applied to the entire system with a time constant of 1.0 ps and a compressibility of 4.46×10 −5  bar −1 . Finally, a 1000 ns production simulation without any restriction was conducted used the same parameters as for equilibration. To enable a 4 fs time step on GPU infrastructure, the hydrogen mass of the protein and sugars were repartitioned to 3.024 daltons using ParmEd (4) while the mass of the atoms to which they are bonded were adjusted by the amount required to leave the total mass unchanged. The structure of wild type Omalizumab Fab in complex with IgE Fc region was modelled from the crystal complex structure of Omalizumab-mutant3 with IgE Fc by virtually mutating R81 and R83 in the antibody light chain to wildtype serine and asparagine, respectively. The MD simulation was conducted using the same setup protocol as for mutant3. 
     AmberTools cpptraj module (7) in was used for clustering analysis of the MD trajectories for both omalizumab Fab3 and omalizumab Fab in complex with IgE Fc. Hierarchical agglomerative algorithm was adopted with average-linkage distance between each cluster is below 2.0 Å. The distance between frames was calculated via best-fit coordinate RMSD between the Cα-atoms in antibody light chain V-region residues. The clustering was conducted only for every 10 frame and all other frames were added to clusters based on how close they are to cluster centroids after clustering. 
     REFERENCES 
     
         
         1. Molecular Operating Environment (MOE), 2014.09; Chemical Computing Group Inc., 1010 Sherbooke St. West, Suite #910, Montreal, QC, Canada, H3A 2R7, 2015. 
         2. Case, D. A., et al. AMBER 2014, 2014, University of California, San Francisco. 
         3. Kirschner, K. et al. GLYCAM06: A generalizable biomolecular force field. Carbohydrates.  J. Comput. Chem.,  2008, 29, 622-655. 
         4. http://parmed.githubio/ParmEd/html/index html 
       
    
     Results: 
     To study how S81R and Q83R mutations in light chain of omalizumab Fab3 affect the interaction with IgE, a one-microsecond molecular dynamic simulation was performed for omalizumab Fab and omalizumab Fab3 in complex with IgE Fc structures, respectively. The trajectory snapshopts were clustered and the top 2 populated cluster center structures were analyzed, which clearly demonstrates that in both clusters, the two arginine mutations in mutant3 form intense hydrogen-bond networks with residue D278 and S280 in the adjacent IgE Cε2 domain, respectively. The visual inspection of the trajectory confirms that the R81-S280 and R83-D278 pairwise interactions are much conserved and stable during simulation therefore the unbent Cε2 conformations relative to the closer Cε3 domain as disclosed in the crystal structure is frozen via the antibody bridging. Interestingly, although the double arginine mutations are spatially adjacent to Cε2 D278 and S280 in the crystal structure of omalizumab Fab3, there are not directly hydrogen-bonded as suggested in MD simulation. For omalizumab Fab, as expected the hydrogen bond networks as seen in mutant3 are missing in the top 2 cluster center structures. Visual trajectory inspection shows that Cε2 domains become less tethered to the antibody light chain framework and thus their relative positions to the closer Cε3 domain are more variable than that of omalizumab Fab3. In addition to this, the interface between omalizumab Fab3 and IgE-Fc in the complex of the same was subjected to an IOTA analysis (IOTA is a statistical potential tool for determining the probability of a given contact atom type at a protein interface or binding site) which predicted that light chain position S56, S64 and S71 in omalizumab Fab3 (with reference to SEQ ID NO: 113) could be mutated to increase the affinity of omaliuzumab for IgE-Fc and would thus enhance the effect predicted to be seen for the S81R and Q38R mutations detailed above. 
     Conclusion: 
     In summary MD simulation proposes the hypothesis that S81R and Q38R mutations facilitates localization of IgE Cε2 domains to the antibody by direct electrostatic and hydrogen-bond interactions with adjacent D278 and S280 in Cε2, and affect IgE plasticity Fc by locking up the Cε2 unbent conformation. This is coupled with the statistical methodology that predicts the S64M mutation and S56 (changed to D, E, Q or R) and S71 (changed to D, E or M) would have a similar effect. 
     Example 6: Measuring Affinity of Anti-IgE Fabs for IgE-Fc by Biacore 
     The Biacore technology measures the interaction between biomolecules without the requirement for labelling. One of the interactants, termed the ligand, is either immobilized directly on or captured to the sensor surface while the other, termed the analyte, flows in solution over the captured surface. The sensor detects the change in mass at the sensor surface as the analyte binds to the ligand and when the analyte dissociates from the ligand. These correspond to both the association and dissociation processes. In the kinetic assay the anti-IgE Fab is the ligand and is captured to the sensor surface. IgE-Fc is the analyte and is captured by the anti-IgE Fab. The association and dissociation of IgE-Fc from the captured anti-IgE Fab is monitored either with IgE-Fc flowing over the sensor surface (association phase) or buffer flowing over the sensor surface (dissociation phase). Details of the method are as follows: 
     Instrument: Biacore 3000, GE Healthcare AB, Uppsala, Sweden 
     Sensor chip: CMS. Catalogue number BR100399 
     BIAnormalising solution: 70% (w/w) glycerol. Part of the BIAmaintenance Kit. Catalogue number BR100651. The BIAmaintenance kit was stored at 4° C. 
     Amine Coupling Kit: Catalogue number BR100633. Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) made up to 75 mg/mL in distilled water and stored in 200 uL aliquots at −70° C. N-Hydroxysuccinimide (NHS) made up to 11.5 mg/mL in distilled water and stored in 200 uL aliquots at −70° C. Ethanolamine hydrochloride-NaOH pH 8.5 stored at 4° C. 
     Buffers: Running buffer is HBS-EP (being 10 mM HEPES pH 7.4, 150 mM NaCl 3 mM EDTA, 0.05% Surfactant P20, reconstituted from 10× stock solution): Catalogue number BR100669. Immobilization buffer is Acetate 4.0 (being 10 mM sodium acetate pH 4.0). Catalogue number BR100349. Buffer stored at 4° C. 
     Ligand: anti-IgE Fabs were the ligand. These were transiently expressed as a recombinant protein in HEK-293 cells and used without further purification. 
     Analyte: IgE-Fc, being the Fc portion of human IgE, expressed as a recombinant protein in CHO cells and purified. Wild-type human IgE-Fc (Cε2-Cε4 domains with numbering V224-K547 according to Dorrington &amp; Bennich (1978) Immunol. Rev. 41:3-25) carrying a C225A mutation was used (Seq. ID No. 108). 
     Fab 2  fragment of a goat anti-human IgG1 (Fab2 fragment specific) (Jackson Immunolabs, catalogue number 109-006-097) was immobilized to sensor surface by amine coupling using standard methods. Anti-IgE Fab was diluted in running buffer (HBS-EP) and approximately 200 resonance units were captured to the surface. IgE-Fc was diluted in running buffer and a serial dilution series from 2 nM to 125 pM was passed over the captured anti-IgE Fab. The association phase was for 180 seconds and the dissociation phase was for 300 seconds. The sensor surface was regenerated with a 60 second exposure to 40 mM HCl, followed by a 60 second exposure to 10 mM NaOH and then a further 60 second exposure to 40 mM HCl. All binding data was processed with double referencing using the BiaEvaluation software following standard procedures. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Affinity of omalizumab Fab and mutants for IgE-Fc 
               
               
                 as measured in a Biacore kinetic assay. The dissociation 
               
               
                 constant (KD) calculated as kd/ka. 
               
            
           
           
               
               
               
               
            
               
                   
                 ka 
                 kd 
                 KD 
               
               
                 Sample 
                 (1/Ms) ×10{circumflex over ( )}6 
                 (1/s) ×10{circumflex over ( )}−3 
                 (nM) 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 omalizumab Fab (wildtype) 
                 1.97 ± 0.021 
                 2.10 ± 0.005 
                 1.07 
               
               
                 omalizumab Fab 1 
                 2.17 ± 0.021 
                 1.95 ± 0.005 
                 0.902 
               
               
                 omalizumab Fab 2 
                 2.21 ± 0.029 
                 2.91 ± 0.007 
                 1.32 
               
               
                 omalizumab Fab 3 
                 2.36 ± 0.021 
                 1.78 ± 0.005 
                 0.754 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Affinity of omalizumab Fab (WT) and mutants for IgE-Fc as 
               
               
                 measured in a Biacore kinetic assay. The dissociation constant 
               
               
                 (KD) calculated as kd/ka. The four mutants are in the wild 
               
               
                 type sequence background (light chain Seq, ID No 20), 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 ka 
                 kd 
                 KD 
               
               
                   
                 Sample 
                 (×10{circumflex over ( )}6) 1/Ms 
                 (×10{circumflex over ( )}−3) 1/s 
                 (pM) 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 WT 
                 2.86 
                 1.42 
                 496 
               
               
                   
                 S56D 
                 3.26 
                 0.713 
                 219 
               
               
                   
                 S56E 
                 2.86 
                 0.717 
                 251 
               
               
                   
                 S71E 
                 2.57 
                 0.583 
                 226 
               
               
                   
                 S71D 
                 2.51 
                 0.696 
                 278 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7a 
               
             
            
               
                   
               
               
                 Affinity of omalizumab Fab (WT) and mutants for IgE-Fc 
               
               
                 as measured in a Biacore kinetic assay. The dissociation 
               
               
                 constant (KD) calculated as kd/ka. The mutations are 
               
               
                 with reference to the light chain Seq. ID No 20). 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 ka (×10{circumflex over ( )}6) 
                 kd 
                 KD 
               
               
                   
                 Sample 
                 1/Ms 
                 (×10{circumflex over ( )}−3) 1/s 
                 (pM) 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 WT 
                 2.25 
                 16.2 
                 721 
               
               
                   
                 S71E 
                 1.7 
                 6.25 
                 368 
               
               
                   
                 S56D 
                 2.17 
                 7.09 
                 327 
               
               
                   
                 S71D, S56D 
                 2.13 
                 8.11 
                 380 
               
               
                   
                 S71E, S56D 
                 1.85 
                 8.17 
                 443 
               
               
                   
                 S71M, S56D 
                 2.32 
                 5.45 
                 238 
               
               
                   
                 S71D, S56E 
                 1.96 
                 8.33 
                 426 
               
               
                   
                 S71E, S56E 
                 1.94 
                 6.66 
                 343 
               
               
                   
                 S71M, S56E 
                 1.76 
                 4.54 
                 258 
               
               
                   
                 S71D, S56Q 
                 1.85 
                 6.12 
                 331 
               
               
                   
                 S71E, S56Q 
                 1.87 
                 5.03 
                 268 
               
               
                   
                 S71M, S56Q 
                 1.44 
                 4.11 
                 286 
               
               
                   
                 S71D, S56R 
                 1.5 
                 4.86 
                 325 
               
               
                   
                 S71E, S56R 
                 1.65 
                 4.63 
                 281 
               
               
                   
                 S71M, S56R 
                 1.05 
                 5.58 
                 534 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7b 
               
             
            
               
                   
               
               
                 Affinity of omalizmab Fab for IgE-Fc as measured in a Biacore kinetic assay. The 
               
               
                 dissociation constant (KD) is calculated as kd/ka. The mutations are in the light 
               
               
                 chain (with reference to Seq. ID No 20). The light chain variable region sequences 
               
               
                 of the Fab mutants are indicated in the left hand column (VL Seq. ID No.). 
               
            
           
           
               
               
               
               
               
               
            
               
                 VL Seq 
                   
                 ka 
                 kd 
                 KD 
                 KD 
               
               
                 ID No. 
                 Sample 
                 (1/Ms) 
                 (1/s) 
                 (M) 
                 (pM) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 20 
                 Omalizumab Fab wt 
                 2.52E+06 
                 2.38E−03 
                 9.42E−10 
                 942 
               
               
                 132 
                 S64M_S81R_Q83R_S56D_S71M 
                 3.23E+06 
                 9.79E−04 
                 3.04E−10 
                 304 
               
               
                 139 
                 S64K_S81R_Q83R_S56D_S71M 
                 2.85E+06 
                 1.02E−03 
                 3.58E−10 
                 358 
               
               
                 145 
                 S64Q_S81R_Q83R_S56D_S71M 
                 2.99E+06 
                 9.12E−04 
                 3.05E−10 
                 305 
               
               
                 146 
                 S64R_S81R_Q83R_S56D_D71M 
                 2.67E+06 
                 6.27E−04 
                 2.35E−10 
                 235 
               
               
                 158 
                 S64M_S81R_Q83R_D56D_D71M_S67W_S80N 
                 3.19E+06 
                 5.40E−04 
                 1.69E−10 
                 169 
               
               
                 159 
                 S64M_S81R_Q83R_D56D_D71M_S67Y_S80N 
                 3.07E+06 
                 4.69E−04 
                 1.53E−10 
                 153 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7c 
               
             
            
               
                   
               
               
                 Affinity of omalizmab Fab mutant for IgE-Fc as measured in a Biacore kinetic assay. The dissociation 
               
               
                 constant (KD) is calculated as kd/ka. The mutations are in the light chain (with reference 
               
               
                 to Seq. ID No 20). The samples have been subjected to a forced oxidation study and the levels 
               
               
                 of methionine oxidation at each time point determine by mass spectrometry. 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                 Time 
                 ka 
                 kd 
                 KD 
                 KD 
               
               
                 Sample 
                 % H2O2 
                 Course 
                 (1/Ms) 
                 (1/s) 
                 (M) 
                 (pM) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 S64M_S81R_Q83R_S56D_S71M 
                 baseline 
                   
                   
                 3.70E+06 
                 1.18E−03 
                  3.2E−10 
                 320 
               
               
                 (Seq ID No. 132) 
               
               
                   
                 0.00% 
                 4 
                 hours 
                 3.43E+06 
                 1.38E−03 
                 4.03E−10 
                 403 
               
               
                   
                   
                 1 
                 Day 
                 3.40E+06 
                 1.46E−03 
                  4.3E−10 
                 430 
               
               
                   
                   
                 3 
                 Days 
                 3.37E+06 
                 1.38E−03 
                 4.09E−10 
                 409 
               
               
                   
                   
                 7 
                 Days 
                 3.25E+06 
                 1.36E−03 
                 4.18E−10 
                 418 
               
               
                   
                   
                 14 
                 Days 
                 3.08E+06 
                 1.22E−03 
                 3.97E−10 
                 397 
               
               
                   
                 0.1 
                 4 
                 hours 
                 3.46E+06 
                 1.44E−03 
                 4.18E−10 
                 418 
               
               
                   
                   
                 1 
                 Day 
                 3.41E+06 
                 1.26E−03 
                 3.71E−10 
                 371 
               
               
                   
                   
                 3 
                 Days 
                 3.49E+06 
                 1.23E−03 
                 3.53E−10 
                 353 
               
               
                   
                   
                 7 
                 Days 
                 5.64E+06 
                 1.36E−03 
                 2.41E−10 
                 241 
               
               
                   
                   
                 14 
                 Days 
                 3.64E+06 
                 1.20E−03 
                 3.31E−10 
                 331 
               
               
                   
               
            
           
         
       
     
     Conclusion: 
     These data demonstrate that mutated forms of omalizumab Fab can increase the affinity of omalizumab Fab for IgE-Fc. The best combination of mutations in terms of improved affinity is S71M combined with S56D. This increase in affinity is principally caused by a decrease in the dissociation rate of the Fab from IgE-Fc. The affinity of omalizumab Fab1 (with S81R, Q83R mutations [S77R, Q79R according to Kabat numbering]) and Fab3 (with S81R, Q83R, L158P mutations [S77R, Q79R, L154P according to Kabat numbering] with reference to SEQ ID NO; 125) is also improved given the improved interaction of the antibody with IgE-Fc Cε2 compared with the unmutated omalizumab Fab. 
     Example 7: Measuring of Accelerated Dissociation of IgE-Fc from Immobilized sFcεRIα by Biacore 
     The effect of anti-IgE Fabs upon the dissociation of IgE-Fc from sFcεRIα was measured using the methods outlined in Example 2 (assay method 2). All anti-IgE Fabs had been expressed in HEK-29s Fabs, purified by standard methods and quantified by absorbance at 280 nm using the calculated molar extinction coefficients. In this assay the concentration of IgE-Fc was 2 nM and the dissociation time 200 seconds. The amount of dissociation of IgE-Fc from the immobilized sFcεRIα was calculated as a function of the initial binding amount and the rate of dissociation was calculated as the amount of loss of IgE-Fc from the immobilized sFcεRIα, normalized for the initial binding amount as a function of elapsed time. 
     S80N (pdb numbering) is thought to interact with D278 of Cε2 IgE, and S67W/Y (pdb numbering) is thought to interact with T298 of Cε2 IgE. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 calculation of the amount of dissociation of IgE-Fc from immobilized sFcεRIα. 
               
               
                 Initial binding of IgE to sFcεRIα is normalized to 100% and dissociation calculated 
               
               
                 relative to that. The apparent dissociation rate is based on an assumed single rate process. 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                 RU of 
                 RU of 
                   
                 apparent 
               
               
                   
                   
                 IgE-Fc 
                 IgE-Fc after 
                 % 
                 dissociation 
               
               
                 Sample 
                 mutations 
                 captured 
                 dissociation 
                 dissociation 
                 rate (1/s) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 omalizumab Fab 3 
                 S81R_Q83R_L158P 
                 152.1 
                 125.9 
                 17.0 
                 6.25E−04 
               
               
                 sample 1 
                 S64M_S81R_Q83R_L158P_56D_71M 
                 135.3 
                 71.0 
                 47.5 
                 2.15E−03 
               
               
                 sample 2 
                 S64M_S81R_Q83R_L158P_56E_71M 
                 120.5 
                 71.6 
                 40.6 
                 1.74E−03 
               
               
                 sample 3 
                 S64M_S81R_Q83R_L158P_56Q_71M 
                 97.5 
                 65.4 
                 33.0 
                 1.33E−03 
               
               
                 sample 4 
                 S64M_S81R_Q83R_L158P_56Q_71E 
                 87.6 
                 62.1 
                 29.1 
                 1.15E−03 
               
               
                 sample 5 
                 S64M_S81R_Q83R_L158P_56R_71E 
                 80.0 
                 64.4 
                 19.5 
                 7.24E−04 
               
               
                 sample 6 
                 S64M_S81R_Q83R_56D_71M 
                 85.9 
                 50.9 
                 40.7 
                 1.74E−03 
               
               
                 sample 7 
                 S64M_S81R_Q83R_56E_71M 
                 101.8 
                 66.6 
                 34.6 
                 1.42E−03 
               
               
                 sample 8 
                 S64M_S81R_Q83R_56Q_71M 
                 109.1 
                 77.6 
                 28.9 
                 1.14E−03 
               
               
                 sample 9 
                 S64M_S81R_Q83R_56Q_71E 
                 107.8 
                 78.0 
                 27.6 
                 1.08E−03 
               
               
                 sample 10 
                 S64M_S81R_Q83R_56R_71E 
                 102.8 
                 84.3 
                 18.1 
                 6.64E−04 
               
               
                 sample 11 
                 S56D_S64M_S71E_S81R_Q83R 
                 156.7 
                 110.5 
                 29.5 
                 1.17E−03 
               
               
                 sample 12 
                 S56D_S64M_S71E_S81R_Q83R_S67W_S80N 
                 155.1 
                 92.0 
                 40.7 
                 1.74E−03 
               
               
                 sample 13 
                 S56D_S64M_S71E_S81R_Q83R_S67Y_S80N 
                 137.6 
                 71.5 
                 48.0 
                 2.18E−03 
               
               
                 buffer control 
                   
                 126.3 
                 116.6 
                 7.8 
                 2.70E−04 
               
               
                   
               
            
           
         
       
     
     Conclusion: 
     These data demonstrate that a mutated form of omalizumab Fab can accelerate the dissociation of IgE from an immobilized form of the high affinity IgE receptor, FcεRI. The mutations in the light chain (Seq. ID No 20) that enable this include, but not necessarily limited to, mutations at positions S56, S64, S67, S71, S80, S81, Q83 &amp; L158 (S52, S60, S63, S67, S76, S77, Q79 &amp; L154, respectively, according to Kabat numbering). 
     Example 8: Forced Oxidation of Mutated Omalizumab Fab 
     Anti-IgE Fab samples were subjected to a forced oxidation regime to ascertain the effect of oxidising methionines in the light chain variable region on the affinity of the Fab for IgE-Fc and the ability to accelerate the dissociation of the IgE-Fc:sFcεRIα complex. Mutated omalizumab Fab was incubated with 0.1% and 1% (v/v) hydrogen peroxide for up to 14 days at room temperature. After incubation the samples were buffer exchanged back into PBS pH 7.4 and the concentration determined by absorbance at 280 nm using a calculated extinction coefficient. Mass spectral analysis to determine the amount of oxidation of the light chain variable regions methionines was performed by reduction and alkylation of the material under denaturing conditions followed by trypsin digestion (50 ug/mL Trypsin for 180 minutes at 37° C. followed by TFA quench) and then analysis by LC-MS using a Thermo Orbitrap Q Exactive Plus mass spectrometer. The percentage of oxidised methionine is calculated relative to the assumption that there is no methionine oxidation at the time of synthesis and compared with reference material that has been held at 4° C. 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 the percentage of oxidised methionine in the light chain variable 
               
               
                 region of the mutated omalizumab Fab. This omalizumab Fab mutant 
               
               
                 contains the following mutations: S64M_S81R_Q83R_S56D_S71M 
               
               
                 (with respect to Seq ID No. 20). 
               
            
           
           
               
               
            
               
                   
                 % oxidized methionine present at each time point 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 IgE 
                 4 
                 1 
                 3 
                 7 
                 14 
                 IgE 
               
               
                   
                 Control 
                 hours 
                 Day 
                 Days 
                 Days 
                 Days 
                 Control 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 M37 
                 0.30% 
                 0.40% 
                 0.30% 
                 0.40% 
                 0.60% 
                 0.90% 
                 0.30% 
               
               
                 (LC) 
               
               
                 M64 
                 18.10% 
                 81.70% 
                 99.70% 
                 99.90% 
                 99.90% 
                 99.90% 
                 20.00% 
               
               
                 (LC) 
               
               
                 M71 
                 6.70% 
                 90.00% 
                 99.70% 
                 99.80% 
                 99.80% 
                 99.90% 
                 6.90% 
               
               
                 (LC) 
               
               
                   
               
            
           
         
       
     
     Conclusion: 
     These data indicate that it is possible to generate a mutated omalizumab Fab that has essentially complete oxidation of the critical light chain methionines (M64 and M71). Based on these data, material from the Day1, Day3 and Day 7 samples were pooled and used to determine the impact of the methionine oxidation on the affinity of the mutated omalizumab Fab for IgE-Fc and the ability to accelerate the dissociation of the IgE-Fc:sFcεRIα complex. 
     Example 9: Measuring Accelerated Dissociation of IgE-Fc from sFcεRIα by FRET 
     The effect of anti-IgE Fabs upon the dissociation of IgE-Fc from sFcεRIα was measured in a homogeneous FRET assay. All anti-IgE Fabs had been expressed in HEK-293 cells and purified by standard methods and quantified by absorbance at 280 nm using the calculated molar extinction methods. The FRET assay used Tb labelled IgE-Fc as the donor and Alexa488 labelled sFcεRIα as the acceptor. Both reagents were mixed and equilibrated at room temperature for 60 minutes at a final assay concentration of 1 nM. Anti-IgE Fabs were added to the mix at a final assay concentration of 500 nM and the fluorescence read (excitation at 330 nm, emission at 495 and 520 nm) every 20 minutes for 800 minutes. Fluorescence emission was plotted as a function of time and the dissociation rate of IgE-Fc from sFcεRIα calculated as the half-life of the complex. These data are reported in Tables 10 and 11. 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 The effect of anti-IgE Fabs (WT and mutants) on the dissociation 
               
               
                 of IgE-Fc from sFcεRIα in a homogeneous FRET assay. 
               
               
                 The dissociation of the IgE-Fc:sFcεRIα complex is a 
               
               
                 two phase decay. The fast, initial phase is the phase in which 
               
               
                 the majority of the IgE-Fc:sFcεRIα complex dissociates 
               
               
                 and from which the half-life of the complex is determined.. The 
               
               
                 mutations are in the light chain (with reference to Seq. ID No 
               
               
                 20). The light chain variable region sequences of the Fab mutants 
               
               
                 are indicated in the left hand column (VL Seq. ID No.). 
               
            
           
           
               
               
               
            
               
                   
                   
                 half-life 
               
               
                   
                   
                 of 
               
               
                 VL-Seq 
                   
                 complex 
               
               
                 ID No. 
                 Sample 
                 (min) 
               
               
                   
               
            
           
           
               
               
               
            
               
                 20 
                 Omalizumab Fab wt 
                 95.34 
               
               
                 132 
                 S64M_S81R_Q83R_S56D_S71M 
                 22.94 
               
               
                 145 
                 S64K_S81R_Q83R_S56D_S71M 
                 29.54 
               
               
                 146 
                 S64Q_S81R_Q83R_S56D_S71M 
                 28.1 
               
               
                 139 
                 S64R_S81R_Q83R_S56D_S71M 
                 19.26 
               
               
                 158 
                 S64M_S81R_Q83R_S56D_S71M_S67W_S80N 
                 17.02 
               
               
                 159 
                 S64M_S81R_Q83R_S56D_S71M_S67Y_S80N 
                 17 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 The effect of methionine oxidation upon the ability of 
               
               
                 anti-IgE Fabs to dissociate IgE-Fc from sFcεRIα 
               
               
                 in a homogeneous FRET assay. The dissociation of the IgE- 
               
               
                 Fc:sFcεRIα complex is a two phase decay. The fast, 
               
               
                 initial phase is the phase in which the majority of the 
               
               
                 IgE-Fc:sFcεRIα complex dissociates and from which 
               
               
                 the half-life of the complex is determined. 
               
            
           
           
               
               
            
               
                 Sample 
                 Half-life of complex (min) 
               
               
                   
               
            
           
           
               
               
            
               
                 buffer 
                 1240 
               
               
                 S64M_S81R_Q83R_S56D_S71M 
                 10.8 
               
               
                 S64M_S81R_Q83R_S56D_Q71M forced 
                 17.3 
               
               
                 oxidation 
               
               
                   
               
            
           
         
       
     
     Conclusions: 
     These data demonstrate that the mutated forms of omalizumab are able to dissociate IgE-Fc from sFcεRIα at a faster rate than the wild type sequence. In particular, mutations in the light chain (with reference to Seq. ID No 20) that enable this include, but not necessarily limited to, mutations at positions S64 and S67. Furthermore, oxidation of the methionine (M64 and M71, Seq. ID No 20) has no significant effect on the ability of the Fab to accelerate the dissociation of the IgE-Fc: sFcεRIα complex. 
     This specification has been described with reference to embodiments of the invention. However, one of ordinary skill in the art appreciates that various modifications and changes can be made without departing from the scope of the invention as set forth in the claims below. Accordingly, the specification is to be regarded in an illustrative rather than a restrictive sense, and all such modifications are intended to be included within the scope of invention. 
     Sequences 
     
       
         
           
               
               
            
               
                 HEAVY CHAIN 
                   
               
               
                 v-region 
               
               
                 Seq. ID No. 1 
                   
               
               
                 EVQLVESGGG LVQPGGSLRL SCAVSGYSIT SGYSWNWIRQ APGKGLEWVA 
                   
               
               
                   
               
               
                 SITYDGSTNY NPSVKGRITI SRDDSKNTFY LQMNSLRAED TAVYYCARGS 
               
               
                   
               
               
                 HYFGHWHFAV WGQGTLVTVS S 
               
               
                   
               
               
                 v-region 
               
               
                 Seq. ID No. 2 
                   
               
               
                 GAAGTGCAGT TGGTGGAGTC GGGTGGAGGG CTGGTGCAGC CTGGCGGTAG 
                   
               
               
                   
               
               
                 CCTGAGGCTG TCCTGTGCCG TGTCCGGATA CTCCATTACC TCCGGCTACT 
               
               
                   
               
               
                 CGTGGAACTG GATCAGACAG GCTCCCGGAA AGGGACTTGA GTGGGTGGCG 
               
               
                   
               
               
                 TCCATCACCT ACGACGGCTC AACCAACTAT AACCCGTCCG TGAAGGGCCG 
               
               
                   
               
               
                 CATCACCATT TCGCGCGACG ACAGCAAGAA TACTTTTTAC CTCCAAATGA 
               
               
                   
               
               
                 ACAGCCTGCG GGCCGAAGAT ACTGCCGTGT ACTACTGCGC GCGGGGATCA 
               
               
                   
               
               
                 CATTACTTCG GGCACTGGCA CTTCGCCGTC TGGGGACAGG GCACCCTCGT 
               
               
                   
               
               
                 CACTGTCTCG AGC 
               
               
                   
               
               
                 v-region with signal sequence underlined and italicised 
               
               
                 Seq. ID No. 3 
                   
               
               
                       EV QLVESGGGLV QPGGSLRLSC AVSGYSITSG 
                   
               
               
                   
               
               
                 YSWNWIRQAP GKGLEWVASI TYDGSTNYNP SVKGRITISR DDSKNTFYLQ 
               
               
                   
               
               
                 MNSLRAEDTA VYYCARGSHY FGHWHFAVWG QGTLVTVSS 
               
               
                   
               
               
                 v-region with signal sequence underlined and italicised 
               
               
                 Seq. ID No. 4 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GAAGTG CAGTTGGTGG AGTCGGGTGG AGGGCTGGTG CAGCCTGGCG 
               
               
                   
               
               
                 GTAGCCTGAG GCTGTCCTGT GCCGTGTCCG GATACTCCAT TACCTCCGGC 
               
               
                   
               
               
                 TACTCGTGGA ACTGGATCAG ACAGGCTCCC GGAAAGGGAC TTGAGTGGGT 
               
               
                   
               
               
                 GGCGTCCATC ACCTACGACG GCTCAACCAA CTATAACCCG TCCGTGAAGG 
               
               
                   
               
               
                 GCCGCATCAC CATTTCGCGC GACGACAGCA AGAATACTTT TTACCTCCAA 
               
               
                   
               
               
                 ATGAACAGCC TGCGGGCCGA AGATACTGCC GTGTACTACT GCGCGCGGGG 
               
               
                   
               
               
                 ATCACATTAC TTCGGGCACT GGCACTTCGC CGTCTGGGGA CAGGGCACCC 
               
               
                   
               
               
                 TCGTCACTGT CTCGAGC 
               
               
                   
               
               
                 v-region + gamma 1 CH1 constant region 
               
               
                 Seq. ID No. 5 
                   
               
               
                 EVQLVESGGG LVQPGGSLRL SCAVSGYSIT SGYSWNWIRQ APGKGLEWVA 
                   
               
               
                   
               
               
                 SITYDGSTNY NPSVKGRITI SRDDSKNTFY LQMNSLRAED TAVYYCARGS 
               
               
                   
               
               
                 HYFGHWHFAV WGQGTLVTVS SASTKGPSVF PLAPSSKSTS GGTAALGCLV 
               
               
                   
               
               
                 KDYFPEPVTV SWNSGALTSG VHTFPAVLQS SGLYSLSSVV TVPSSSLGTQ 
               
               
                   
               
               
                 TYICNVNHKP SNTKVDKKVE PKSC 
               
               
                   
               
               
                 v-region + gamma 1 CH1 constant region 
               
               
                 Seq. ID No. 6 
                   
               
               
                 GAAGTGCAGT TGGTGGAGTC GGGTGGAGGG CTGGTGCAGC CTGGCGGTAG 
                   
               
               
                   
               
               
                 CCTGAGGCTG TCCTGTGCCG TGTCCGGATA CTCCATTACC TCCGGCTACT 
               
               
                   
               
               
                 CGTGGAACTG GATCAGACAG GCTCCCGGAA AGGGACTTGA GTGGGTGGCG 
               
               
                   
               
               
                 TCCATCACCT ACGACGGCTC AACCAACTAT AACCCGTCCG TGAAGGGCCG 
               
               
                   
               
               
                 CATCACCATT TCGCGCGACG ACAGCAAGAA TACTTTTTAC CTCCAAATGA 
               
               
                   
               
               
                 ACAGCCTGCG GGCCGAAGAT ACTGCCGTGT ACTACTGCGC GCGGGGATCA 
               
               
                   
               
               
                 CATTACTTCG GGCACTGGCA CTTCGCCGTC TGGGGACAGG GCACCCTCGT 
               
               
                   
               
               
                 CACTGTCTCG AGCGCTTCTA CAAAGGGCCC ATCGGTCTTC CCCCTGGCAC 
               
               
                   
               
               
                 CCTCCTCCAA GAGCACCTCT GGGGGCACAG CGGCCCTGGG CTGCCTGGTC 
               
               
                   
               
               
                 AAGGACTACT TCCCCGAACC GGTGACGGTG TCGTGGAACT CAGGCGCCCT 
               
               
                   
               
               
                 GACCAGCGGC GTGCACACCT TCCCGGCTGT CCTACAGTCC TCAGGACTCT 
               
               
                   
               
               
                 ACTCCCTCAG CAGCGTGGTG ACCGTGCCCT CCAGCAGCTT GGGCACCCAG 
               
               
                   
               
               
                 ACCTACATCT GCAACGTGAA TCACAAGCCC AGCAACACCA AGGTGGACAA 
               
               
                   
               
               
                 GAAAGTTGAG CCCAAATCTT GT 
               
               
                   
               
               
                 v-region + gamma 1 CH1 constant region with signal sequence 
               
               
                 underlined and italicised 
               
               
                 Seq. ID No. 7 
                   
               
               
                       EV QLVESGGGLV QPGGSLRLSC AVSGYSITSG 
                   
               
               
                   
               
               
                 YSWNWIRQAP GKGLEWVASI TYDGSTNYNP SVKGRITISR DDSKNTFYLQ 
               
               
                   
               
               
                 MNSLRAEDTA VYYCARGSHY FGHWHFAVWG QGTLVTVSSA STKGPSVFPL 
               
               
                   
               
               
                 APSSKSTSGG TAALGCLVKD YFPEPVTVSW NSGALTSGVH TFPAVLQSSG 
               
               
                   
               
               
                 LYSLSSVVTV PSSSLGTQTY ICNVNHKPSN TKVDKKVEPK Sc 
               
               
                   
               
               
                 v-region + gamma 1 CH1 constant region with signal sequence 
               
               
                 underlined and italicised 
               
               
                 Seq. ID No. 8 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GAAGTG CAGTTGGTGG AGTCGGGTGG AGGGCTGGTG CAGCCTGGCG 
               
               
                   
               
               
                 GTAGCCTGAG GCTGTCCTGT GCCGTGTCCG GATACTCCAT TACCTCCGGC 
               
               
                   
               
               
                 TACTCGTGGA ACTGGATCAG ACAGGCTCCC GGAAAGGGAC TTGAGTGGGT 
               
               
                   
               
               
                 GGCGTCCATC ACCTACGACG GCTCAACCAA CTATAACCCG TCCGTGAAGG 
               
               
                   
               
               
                 GCCGCATCAC CATTTCGCGC GACGACAGCA AGAATACTTT TTACCTCCAA 
               
               
                   
               
               
                 ATGAACAGCC TGCGGGCCGA AGATACTGCC GTGTACTACT GCGCGCGGGG 
               
               
                   
               
               
                 ATCACATTAC TTCGGGCACT GGCACTTCGC CGTCTGGGGA CAGGGCACCC 
               
               
                   
               
               
                 TCGTCACTGT CTCGAGCGCT TCTACAAAGG GCCCATCGGT CTTCCCCCTG 
               
               
                   
               
               
                 GCACCCTCCT CCAAGAGCAC CTCTGGGGGC ACAGCGGCCC TGGGCTGCCT 
               
               
                   
               
               
                 GGTCAAGGAC TACTTCCCCG AACCGGTGAC GGTGTCGTGG AACTCAGGCG 
               
               
                   
               
               
                 CCCTGACCAG CGGCGTGCAC ACCTTCCCGG CTGTCCTACA GTCCTCAGGA 
               
               
                   
               
               
                 CTCTACTCCC TCAGCAGCGT GGTGACCGTG CCCTCCAGCA GCTTGGGCAC 
               
               
                   
               
               
                 CCAGACCTAC ATCTGCAACG TGAATCACAA GCCCAGCAAC ACCAAGGTGG 
               
               
                   
               
               
                 ACAAGAAAGT TGAGCCCAAA TCTTGT 
               
               
                   
               
               
                 v-region + gamma 1 full length constant region 
               
               
                 Seq. ID No. 9 
                   
               
               
                 EVQLVESGGG LVQPGGSLRL SCAVSGYSIT SGYSWNWIRQ APGKGLEWVA 
                   
               
               
                   
               
               
                 SITYDGSTNY NPSVKGRITI SRDDSKNTFY LQMNSLRAED TAVYYCARGS 
               
               
                   
               
               
                 HYFGHWHFAV WGQGTLVTVS SASTKGPSVF PLAPSSKSTS GGTAALGCLV 
               
               
                   
               
               
                 KDYFPEPVTV SWNSGALTSG VHTFPAVLQS SGLYSLSSVV TVPSSSLGTQ 
               
               
                   
               
               
                 TYICNVNHKP SNTKVDKKVE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK 
               
               
                   
               
               
                 PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY 
               
               
                   
               
               
                 NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP 
               
               
                   
               
               
                 QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP 
               
               
                   
               
               
                 VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG 
               
               
                   
               
               
                 K 
               
               
                   
               
               
                 v-region + gamma 1 full length constant region 
               
               
                 Seq. ID No. 10 
                   
               
               
                 GAAGTGCAGT TGGTGGAGTC GGGTGGAGGG CTGGTGCAGC CTGGCGGTAG 
                   
               
               
                   
               
               
                 CCTGAGGCTG TCCTGTGCCG TGTCCGGATA CTCCATTACC TCCGGCTACT 
               
               
                   
               
               
                 CGTGGAACTG GATCAGACAG GCTCCCGGAA AGGGACTTGA GTGGGTGGCG 
               
               
                   
               
               
                 TCCATCACCT ACGACGGCTC AACCAACTAT AACCCGTCCG TGAAGGGCCG 
               
               
                   
               
               
                 CATCACCATT TCGCGCGACG ACAGCAAGAA TACTTTTTAC CTCCAAATGA 
               
               
                   
               
               
                 ACAGCCTGCG GGCCGAAGAT ACTGCCGTGT ACTACTGCGC GCGGGGATCA 
               
               
                   
               
               
                 CATTACTTCG GGCACTGGCA CTTCGCCGTC TGGGGACAGG GCACCCTCGT 
               
               
                   
               
               
                 CACTGTCTCG AGCGCTTCTA CAAAGGGCCC CTCCGTGTTC CCGCTCGCTC 
               
               
                   
               
               
                 CATCATCGAA GTCTACCAGC GGAGGCACTG CGGCTCTCGG TTGCCTCGTG 
               
               
                   
               
               
                 AAGGACTACT TCCCGGAGCC GGTGACCGTG TCGTGGAACA GCGGAGCCCT 
               
               
                   
               
               
                 GACCAGCGGG GTGCACACCT TTCCGGCCGT CTTGCAGTCA AGCGGCCTTT 
               
               
                   
               
               
                 ACTCCCTGTC ATCAGTGGTG ACTGTCCCGT CCAGCTCATT GGGAACCCAA 
               
               
                   
               
               
                 ACCTACATCT GCAATGTGAA TCACAAACCT AGCAACACCA AGGTTGACAA 
               
               
                   
               
               
                 GAAAGTCGAG CCCAAATCGT GTGACAAGAC TCACACTTGT CCGCCGTGCC 
               
               
                   
               
               
                 CGGCACCCGA ACTGCTGGGA GGTCCCAGCG TCTTTCTGTT CCCTCCAAAG 
               
               
                   
               
               
                 CCGAAAGACA CGCTGATGAT CTCCCGCACC CCGGAGGTCA CTTGCGTGGT 
               
               
                   
               
               
                 CGTGGACGTG TCACATGAGG ACCCAGAGGT GAAGTTCAAT TGGTACGTGG 
               
               
                   
               
               
                 ATGGCGTCGA AGTCCACAAT GCCAAAACTA AGCCCAGAGA AGAACAGTAC 
               
               
                   
               
               
                 AATTCGACCT ACCGCGTCGT GTCCGTGCTC ACGGTGTTGC ATCAGGATTG 
               
               
                   
               
               
                 GCTGAACGGG AAGGAATACA AGTGCAAAGT GTCCAACAAG GCGCTGCCGG 
               
               
                   
               
               
                 CACCGATCGA GAAAACTATC TCCAAAGCGA AGGGACAGCC TAGGGAACCT 
               
               
                   
               
               
                 CAAGTCTACA CGCTGCCACC ATCACGGGAA GAAATGACTA AGAATCAAGT 
               
               
                   
               
               
                 CTCACTGACT TGTCTGGTGA AGGGGTTTTA CCCTAGCGAC ATTGCCGTGG 
               
               
                   
               
               
                 AGTGGGAATC CAACGGCCAG CCAGAGAACA ACTACAAGAC TACCCCTCCA 
               
               
                   
               
               
                 GTGCTCGACT CGGATGGATC GTTCTTCCTT TACTCGAAGC TCACCGTGGA 
               
               
                   
               
               
                 TAAGTCCCGG TGGCAGCAGG GAAACGTGTT CTCCTGCTCG GTGATGCATG 
               
               
                   
               
               
                 AAGCCCTCCA TAACCACTAT ACCCAAAAGT CGCTGTCCCT GTCGCCGGGA 
               
               
                   
               
               
                 AAG 
               
               
                   
               
               
                 v-region + gamma 1 full length constant region with signal 
               
               
                 sequence underlined and italicised 
               
               
                 Seq. ID No. 11 
                   
               
               
                       EV QLVESGGGLV QPGGSLRLSC AVSGYSITSG 
                   
               
               
                   
               
               
                 YSWNWIRQAP GKGLEWVASI TYDGSTNYNP SVKGRITISR DDSKNTFYLQ 
               
               
                   
               
               
                 MNSLRAEDTA VYYCARGSHY FGHWHFAVWG QGTLVTVSSA STKGPSVFPL 
               
               
                   
               
               
                 APSSKSTSGG TAALGCLVKD YFPEPVTVSW NSGALTSGVH TFPAVLQSSG 
               
               
                   
               
               
                 LYSLSSVVTV PSSSLGTQTY ICNVNHKPSN TKVDKKVEPK SCDKTHTCPP 
               
               
                   
               
               
                 CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY 
               
               
                   
               
               
                 VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL 
               
               
                   
               
               
                 PAPIEKTISK AKGQPREPQV YTLPPSREEM TKNQVSLTCL VKGFYPSDIA 
               
               
                   
               
               
                 VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM 
               
               
                   
               
               
                 HEALHNHYTQ KSLSLSPGK 
               
               
                   
               
               
                 v-region + gamma 1 full length constant region with signal 
               
               
                 sequence underlined and italicised 
               
               
                 Seq. ID No. 12 
                   
               
               
                               
                   
               
               
                   
               
               
                    GAAGTG CAGTTGGTGG AGTCGGGTGG AGGGCTGGTG CAGCCTGGCG 
               
               
                   
               
               
                 GTAGCCTGAG GCTGTCCTGT GCCGTGTCCG GATACTCCAT TACCTCCGGC 
               
               
                   
               
               
                 TACTCGTGGA ACTGGATCAG ACAGGCTCCC GGAAAGGGAC TTGAGTGGGT 
               
               
                   
               
               
                 GGCGTCCATC ACCTACGACG GCTCAACCAA CTATAACCCG TCCGTGAAGG 
               
               
                   
               
               
                 GCCGCATCAC CATTTCGCGC GACGACAGCA AGAATACTTT TTACCTCCAA 
               
               
                   
               
               
                 ATGAACAGCC TGCGGGCCGA AGATACTGCC GTGTACTACT GCGCGCGGGG 
               
               
                   
               
               
                 ATCACATTAC TTCGGGCACT GGCACTTCGC CGTCTGGGGA CAGGGCACCC 
               
               
                   
               
               
                 TCGTCACTGT CTCGAGCGCT TCTACAAAGG GCCCCTCCGT GTTCCCGCTC 
               
               
                   
               
               
                 GCTCCATCAT CGAAGTCTAC CAGCGGAGGC ACTGCGGCTC TCGGTTGCCT 
               
               
                   
               
               
                 CGTGAAGGAC TACTTCCCGG AGCCGGTGAC CGTGTCGTGG AACAGCGGAG 
               
               
                   
               
               
                 CCCTGACCAG CGGGGTGCAC ACCTTTCCGG CCGTCTTGCA GTCAAGCGGC 
               
               
                   
               
               
                 CTTTACTCCC TGTCATCAGT GGTGACTGTC CCGTCCAGCT CATTGGGAAC 
               
               
                   
               
               
                 CCAAACCTAC ATCTGCAATG TGAATCACAA ACCTAGCAAC ACCAAGGTTG 
               
               
                   
               
               
                 ACAAGAAAGT CGAGCCCAAA TCGTGTGACA AGACTCACAC TTGTCCGCCG 
               
               
                   
               
               
                 TGCCCGGCAC CCGAACTGCT GGGAGGTCCC AGCGTCTTTC TGTTCCCTCC 
               
               
                   
               
               
                 AAAGCCGAAA GACACGCTGA TGATCTCCCG CACCCCGGAG GTCACTTGCG 
               
               
                   
               
               
                 TGGTCGTGGA CGTGTCACAT GAGGACCCAG AGGTGAAGTT CAATTGGTAC 
               
               
                   
               
               
                 GTGGATGGCG TCGAAGTCCA CAATGCCAAA ACTAAGCCCA GAGAAGAACA 
               
               
                   
               
               
                 GTACAATTCG ACCTACCGCG TCGTGTCCGT GCTCACGGTG TTGCATCAGG 
               
               
                   
               
               
                 ATTGGCTGAA CGGGAAGGAA TACAAGTGCA AAGTGTCCAA CAAGGCGCTG 
               
               
                   
               
               
                 CCGGCACCGA TCGAGAAAAC TATCTCCAAA GCGAAGGGAC AGCCTAGGGA 
               
               
                   
               
               
                 ACCTCAAGTC TACACGCTGC CACCATCACG GGAAGAAATG ACTAAGAATC 
               
               
                   
               
               
                 AAGTCTCACT GACTTGTCTG GTGAAGGGGT TTTACCCTAG CGACATTGCC 
               
               
                   
               
               
                 GTGGAGTGGG AATCCAACGG CCAGCCAGAG AACAACTACA AGACTACCCC 
               
               
                   
               
               
                 TCCAGTGCTC GACTCGGATG GATCGTTCTT CCTTTACTCG AAGCTCACCG 
               
               
                   
               
               
                 TGGATAAGTC CCGGTGGCAG CAGGGAAACG TGTTCTCCTG CTCGGTGATG 
               
               
                   
               
               
                 CATGAAGCCC TCCATAACCA CTATACCCAA AAGTCGCTGT CCCTGTCGCC 
               
               
                   
               
               
                 GGGAAAG 
               
               
                   
               
               
                 FR H1 
               
               
                 Seq. ID No. 13 
                   
               
               
                 EVQLVESGGG LVQPGGSLRL SCAVS 
                   
               
               
                   
               
               
                 CDRH1 
               
               
                 Seq. ID No. 14 
                   
               
               
                 GYSITSGYSW N 
                   
               
               
                   
               
               
                 FR H2 
               
               
                 Seq. ID No. 15 
                   
               
               
                 WIRQAPGKGL EWVA 
                   
               
               
                   
               
               
                 CDRH2 
               
               
                 Seq. ID No. 16 
                   
               
               
                 SITYDGSTNY NPSVKG 
                   
               
               
                   
               
               
                 FR H3 
               
               
                 Seq. ID No. 17 
                   
               
               
                 RITISRDDSK NTFYLQMNSL RAEDTAVYYC AR 
                   
               
               
                   
               
               
                 CDRH3 
               
               
                 Seq. ID No. 18 
                   
               
               
                 GSHYFGHWHF AV 
                   
               
               
                   
               
               
                 FR H4 
               
               
                 Seq. ID No. 19 
                   
               
               
                 WGQGTLVTVS S 
                   
               
               
                   
               
               
                 LIGHT CHAIN 
               
               
                 Omalizumab_v-region 
               
               
                 Seq. ID No. 20 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAASYLES GVPSRFSGSG SGTDFTLTIS SLQPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 Omalizumab_v-region 
               
               
                 Seq. ID No. 21 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAT CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCATA CCTTGAAAGC GGCGTGCCT T   CG CGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCGGGCACCG ATTTCACCCT GACCATTTCG  TCG CTG CAG C 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGAACCAA GGTCGAAATC AAG 
               
               
                   
               
               
                 Omalizumab_v-region with signal sequence underlined and 
               
               
                 italicised 
               
               
                 Seq. ID No. 22 
                   
               
               
                      DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PSRFSGSGSG TDFTLTISSL 
               
               
                   
               
               
                 QPEDFATYYC QQSHEDPYTF GQGTKVEIK 
               
               
                   
               
               
                 Omalizumab_v-region with signal sequence underlined and 
               
               
                 italicised 
               
               
                 Seq. ID No. 23 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTATCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CATACCTTGA AAGCGGCGTG CCT TCG CGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCGGGC ACCGATTTCA CCCTGACCAT TTCG TCG CTG 
               
               
                   
               
               
                   CAG CCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGAA CCAAGGTCGA AATCAAG 
               
               
                   
               
               
                 Omalizumab_v-region + kappa constant region 
               
               
                 Seq. ID No. 24 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAASYLES GVPSRFSGSG SGTDFTLTIS SLQPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI KRTVAAPSVF IFPPSDEQLK SGTASVVCLL NNFYPREAKV 
               
               
                   
               
               
                 QWKVDNALQS GNSQESVTEQ DSKDSTYSLS STLTLSKADY EKHKVYACEV 
               
               
                   
               
               
                 THQGLSSPVT KSFNRGEC 
               
               
                   
               
               
                 Omalizumab_v-region + kappa constant region 
               
               
                 Seq. ID No. 25 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAT CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCATA CCTTGAAAGC GGCGTGCCT T   CG CGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCGGGCACCG ATTTCACCCT GACCATTTCG  TCG CTG CAG C 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGAACCAA GGTCGAAATC AAGCGTACGG TAGCGGCCCC 
               
               
                   
               
               
                 ATCTGTCTTC ATCTTCCCGC CATCTGATGA GCAGTTGAAA TCTGGAACTG 
               
               
                   
               
               
                 CCTCTGTTGT GTGCCTGCTG AATAACTTCT ATCCCAGAGA GGCCAAAGTA 
               
               
                   
               
               
                 CAGTGGAAGG TGGATAACGC C CTC CAATCG GGTAACTCCC AGGAGAGTGT 
               
               
                   
               
               
                 CACAGAGCAG GACAGCAAGG ACAGCACCTA CAGCCTCAGC AGCACCCTGA 
               
               
                   
               
               
                 CGCTGAGCAA AGCAGACTAC GAGAAACACA AAGTCTACGC CTGCGAAGTC 
               
               
                   
               
               
                 ACCCATCAGG GCCTGAGCTC GCCCGTCACA AAGAGCTTCA ACAGGGGAGA GTGT 
               
               
                   
               
               
                 Omalizumab_v-region + kappa constant region with signal sequence 
               
               
                 underlined and italicised 
               
               
                 Seq. ID No. 26 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PSRFSGSGSG TDFTLTISSL 
               
               
                   
               
               
                 QPEDFATYYC QQSHEDPYTF GQGTKVEIKR TVAAPSVFIF PPSDEQLKSG 
               
               
                   
               
               
                 TASVVCLLNN FYPREAKVQW KVDNALQSGN SQESVTEQDS KDSTYSLSST 
               
               
                   
               
               
                 LTLSKADYEK HKVYACEVTH QGLSSPVTKS FNRGEC 
               
               
                   
               
               
                 Omalizumab_v-region + kappa constant region with signal sequence 
               
               
                 underlined and italicised 
               
               
                 Seq. ID No. 27 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTATCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CATACCTTGA AAGCGGCGTG CCT TCG CGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCGGGC ACCGATTTCA CCCTGACCAT TTCG TCG CTG 
               
               
                   
               
               
                   CAG CCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGAA CCAAGGTCGA AATCAAGCGT ACGGTAGCGG 
               
               
                   
               
               
                 CCCCATCTGT CTTCATCTTC CCGCCATCTG ATGAGCAGTT GAAATCTGGA 
               
               
                   
               
               
                 ACTGCCTCTG TTGTGTGCCT GCTGAATAAC TTCTATCCCA GAGAGGCCAA 
               
               
                   
               
               
                 AGTACAGTGG AAGGTGGATA ACGCC CTC CA ATCGGGTAAC TCCCAGGAGA 
               
               
                   
               
               
                 GTGTCACAGA GCAGGACAGC AAGGACAGCA CCTACAGCCT CAGCAGCACC 
               
               
                   
               
               
                 CTGACGCTGA GCAAAGCAGA CTACGAGAAA CACAAAGTCT ACGCCTGCGA 
               
               
                   
               
               
                 AGTCACCCAT CAGGGCCTGA GCTCGCCCGT CACAAAGAGC TTCAACAGGG GAGAGTGT 
               
               
                   
               
               
                 FR L1 
               
               
                 Seq. ID No. 28 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITC 
                   
               
               
                   
               
               
                 CDRL1 
               
               
                 Seq. ID No. 29 
                   
               
               
                 RASQSVDYDG DSYMN 
                   
               
               
                   
               
               
                 FR L2 
               
               
                 Seq. ID No. 30 
                   
               
               
                 WYQQKPGKAP KLLIY 
                   
               
               
                   
               
               
                 CDRL2 
               
               
                 Seq. ID No. 31 
                   
               
               
                   
               
               
                 AASYLES 
                   
               
               
                   
               
               
                 FR L3 
               
               
                 Seq. ID No. 32 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIS SLQPEDFATY YC 
                   
               
               
                   
               
               
                 CDRL3 
               
               
                 Seq. ID No. 33 
                   
               
               
                 QQSHEDPYT 
                   
               
               
                   
               
               
                 Seq. ID No. 34 
                   
               
               
                 FR L4 
                   
               
               
                 FGQGTKVEIK 
               
               
                   
               
               
                 MUTANT LIGHT CHAIN 
               
               
                 Seq. ID No. 35 
                   
               
               
                 S60M_S77R_Q79R_v-region (Kabat) 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
               
               
                   
               
               
                 LIYAASYLES GVPMRFSGSG SGTDFTLTIS RLRPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 S60M_S77R_Q79R_v-region (Kabat) 
               
               
                 Seq. ID No. 36 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAT CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCATA CCTTGAAAGC GGCGTGCCTA TGCGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCGGGCACCG ATTTCACCCT GACCATTTCG AGACTGAGGC 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGAACCAA GGTCGAAATC AAG 
               
               
                   
               
               
                 S60M_S77R_Q79R_v-region with signal sequence underlined and 
               
               
                 italicised (Kabat) 
               
               
                 Seq. ID No. 37 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PMRFSGSGSG TDFTLTISRL 
               
               
                   
               
               
                 RPEDFATYYC QQSHEDPYTF GQGTKVEIK 
               
               
                   
               
               
                 S60M_S77R_Q79R_v-region with signal sequence underlined and 
               
               
                 italicised (Kabat) 
               
               
                 Seq. ID No. 38 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTATCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CATACCTTGA AAGCGGCGTG CCTATGCGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCGGGC ACCGATTTCA CCCTGACCAT TTCGAGACTG 
               
               
                   
               
               
                 AGGCCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGAA CCAAGGTCGA AATCAAG 
               
               
                   
               
               
                 S60M_S77R_Q79R_v-region + kappa constant region including L154P 
               
               
                 (Kabat) 
               
               
                 Seq. ID No. 39 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAASYLES GVPMRFSGSG SGTDFTLTIS RLRPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI KRTVAAPSVF IFPPSDEQLK SGTASVVCLL NNFYPREAKV 
               
               
                   
               
               
                 QWKVDNAPQS GNSQESVTEQ DSKDSTYSLS STLTLSKADY EKHKVYACEV 
               
               
                   
               
               
                 THQGLSSPVT KSFNRGEC 
               
               
                   
               
               
                 S60M_S77R_Q79R_v-region + kappa constant region including L154P 
               
               
                 (Kabat) 
               
               
                 Seq. ID No. 40 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAT CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCATA CCTTGAAAGC GGCGTGCCTA TGCGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCGGGCACCG ATTTCACCCT GACCATTTCG AGACTGAGGC 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGAACCAA GGTCGAAATC AAGCGTACGG TAGCGGCCCC 
               
               
                   
               
               
                 ATCTGTCTTC ATCTTCCCGC CATCTGATGA GCAGTTGAAA TCTGGAACTG 
               
               
                   
               
               
                 CCTCTGTTGT GTGCCTGCTG AATAACTTCT ATCCCAGAGA GGCCAAAGTA 
               
               
                   
               
               
                 CAGTGGAAGG TGGATAACGC CCCGCAATCG GGTAACTCCC AGGAGAGTGT 
               
               
                   
               
               
                 CACAGAGCAG GACAGCAAGG ACAGCACCTA CAGCCTCAGC AGCACCCTGA 
               
               
                   
               
               
                 CGCTGAGCAA AGCAGACTAC GAGAAACACA AAGTCTACGC CTGCGAAGTC 
               
               
                   
               
               
                 ACCCATCAGG GCCTGAGCTC GCCCGTCACA AAGAGCTTCA ACAGGGGAGA GTGT 
               
               
                   
               
               
                 S60M S77R Q79R_v-region + kappa constant region including L154P 
               
               
                 with signal sequence underlined and italicised (Kabat) 
               
               
                 Seq. ID No. 41 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PMRFSGSGSG TDFTLTISRL 
               
               
                   
               
               
                 RPEDFATYYC QQSHEDPYTF GQGTKVEIKR TVAAPSVFIF PPSDEQLKSG 
               
               
                   
               
               
                 TASVVCLLNN FYPREAKVQW KVDNAPQSGN SQESVTEQDS KDSTYSLSST 
               
               
                   
               
               
                 LTLSKADYEK HKVYACEVTH QGLSSPVTKS FNRGEC 
               
               
                   
               
               
                 S60M S77R Q79R_v-region + kappa constant region including L154P 
               
               
                 with signal sequence underlined and italicised (Kabat) 
               
               
                 Seq. ID No. 42 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTATCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CATACCTTGA AAGCGGCGTG CCTATGCGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCGGGC ACCGATTTCA CCCTGACCAT TTCGAGACTG 
               
               
                   
               
               
                 AGGCCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGAA CCAAGGTCGA AATCAAGCGT ACGGTAGCGG 
               
               
                   
               
               
                 CCCCATCTGT CTTCATCTTC CCGCCATCTG ATGAGCAGTT GAAATCTGGA 
               
               
                   
               
               
                 ACTGCCTCTG TTGTGTGCCT GCTGAATAAC TTCTATCCCA GAGAGGCCAA 
               
               
                   
               
               
                 AGTACAGTGG AAGGTGGATA ACGCCCCGCA ATCGGGTAAC TCCCAGGAGA 
               
               
                   
               
               
                 GTGTCACAGA GCAGGACAGC AAGGACAGCA CCTACAGCCT CAGCAGCACC 
               
               
                   
               
               
                 CTGACGCTGA GCAAAGCAGA CTACGAGAAA CACAAAGTCT ACGCCTGCGA 
               
               
                   
               
               
                 AGTCACCCAT CAGGGCCTGA GCTCGCCCGT CACAAAGAGC TTCAACAGGG GAGAGTGT 
               
               
                   
               
               
                 FR L3 S64M (PDB) S60M (Kabat) 
               
               
                 Seq. ID No. 43 
                   
               
               
                 GVPMRFSGSG SGTDFTLTIS SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R(PDB) S77R (Kabat) 
               
               
                 Seq. ID No. 44 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIS RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 Q83R(PDB) Q79R (Kabat) 
               
               
                 Seq. ID No. 45 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIS SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R(PDB) S60M S77R (Kabat) 
               
               
                 Seq. ID No. 46 
                   
               
               
                 GVPMRFSGSG SGTDFTLTIS RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M Q83R(PDB) S60M Q79R (Kabat) 
               
               
                 Seq. ID No. 47 
                   
               
               
                 GVPMRFSGSG SGTDFTLTIS SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R Q83R(PDB) S77R Q79R (Kabat) 
               
               
                 Seq. ID No. 48 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIS RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R Q83R(PDB) S60M S77R Q79R (Kabat) 
               
               
                 Seq. ID No. 49 
                   
               
               
                 GVPMRFSGSG SGTDFTLTIS RLRPEDFATY YC 
                   
               
               
                   
               
               
                 CDRL2 556D(PDB) 552D (Kabat) 
               
               
                 Seq. ID No. 50 
                   
               
               
                 AADYLES 
                   
               
               
                   
               
               
                 CDRL2 556E(PDB) 552E (Kabat) 
               
               
                 Seq. ID No. 51 
                   
               
               
                 AAEYLES 
                   
               
               
                   
               
               
                 FR L3 S71M(PDB) S67M (Kabat) 
               
               
                 Seq. ID No. 52 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIS SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S71M (PDB) S60M S67M (Kabat) 
               
               
                 Seq. ID No. 53 
                   
               
               
                 GVPMRFSGSG MGTDFTLTIS SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R S71M (PDB) S77R S67M (Kabat) 
               
               
                 Seq. ID No. 54 
                   
               
               
                 GVPSRFSGSG MGTDFTLTIS RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 Q83R S71M (PDB) Q79R S67M (Kabat) 
               
               
                 Seq. ID No. 55 
                   
               
               
                 GVPSRFSGSG MGTDFTLTIS SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R S71M (PDB) S60M S77R S67M (Kabat) 
               
               
                 Seq. ID No. 56 
                   
               
               
                 GVPMRFSGSG MGTDFTLTIS RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M Q83R S71M (PDB) S60M Q79R S67M (Kabat) 
               
               
                 Seq. ID No. 57 
                   
               
               
                 GVPMRFSGSG MGTDFTLTIS SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R Q83R S71M (PDB) S77R Q79R S67M (Kabat) 
               
               
                 Seq. ID No. 58 
                   
               
               
                 GVPSRFSGSG MGTDFTLTIS RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R Q83R S71M (PDB) S60M S77R Q79R S67M (Kabat) 
               
               
                 Seq. ID No. 59 
                   
               
               
                 GVPMRFSGSG MGTDFTLTIS RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S67Y (PDB) S63Y (Kabat) 
               
               
                 Seq. ID No. 60 
                   
               
               
                 GVPSRFYGSG SGTDFTLTIS SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S67Y (PDB) S60M S63Y (Kabat) 
               
               
                 Seq. ID No. 61 
                   
               
               
                 GVPMRFYGSG SGTDFTLTIS SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R S67Y (PDB) S77R S63Y (Kabat) 
               
               
                 Seq. ID No. 62 
                   
               
               
                 GVPSRFYGSG SGTDFTLTIS RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 Q83R S67Y (PDB) Q79R S63Y (Kabat) 
               
               
                 Seq. ID No. 63 
                   
               
               
                 GVPSRFYGSG SGTDFTLTIS SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R S67Y (PDB) S60M S77R S63Y (Kabat) 
               
               
                 Seq. ID No. 64 
                   
               
               
                 GVPMRFYGSG SGTDFTLTIS RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M Q83R S67Y (PDB) S60M Q79R S63Y (Kabat) 
               
               
                 Seq. ID No. 65 
                   
               
               
                 GVPMRFYGSG SGTDFTLTIS SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R Q83R S67Y (PDB) S77R Q79R S63Y (Kabat) 
               
               
                 Seq. ID No. 66 
                   
               
               
                 GVPSRFYGSG SGTDFTLTIS RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R Q83R S67Y (PDB) S60M S77R Q79R S63Y (Kabat) 
               
               
                 Seq. ID No. 67 
                   
               
               
                 GVPMRFYGSG SGTDFTLTIS RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S80N (PDB) S76N (Kabat) 
               
               
                 Seq. ID No. 68 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIN SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S80N (PDB) S60M S76N (Kabat) 
               
               
                 Seq. ID No. 69 
                   
               
               
                 GVPMRFSGSG SGTDFTLTIN SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R S80N (PDB) S77R S76N (Kabat) 
               
               
                 Seq. ID No. 70 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIN RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 Q83R S80N (PDB) Q79R S76N (Kabat) 
               
               
                 Seq. ID No. 71 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIN SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R S80N (PDB) S60M S77R S76N (Kabat) 
               
               
                 Seq. ID No. 72 
                   
               
               
                 GVPMRFSGSG SGTDFTLTIN RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M Q83R S80N (PDB) S60M Q79R S76N (Kabat) 
               
               
                 Seq. ID No. 73 
                   
               
               
                 GVPMRFSGSG SGTDFTLTIN SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R Q83R S80N (PDB) S77R Q79R S76N (Kabat) 
               
               
                 Seq. ID No. 74 
                   
               
               
                 GVPSRFSGSG SGTDFTLTIN RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R Q83R S80N (PDB) S60M S77R Q79R S76N (Kabat) 
               
               
                 Seq. ID No. 75 
                   
               
               
                 GVPMRFSGSG SGTDFTLTIN RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S80N S67Y (PDB) S76N S63Y(Kabat) 
               
               
                 Seq. ID No. 76 
                   
               
               
                 GVPSRFYGSG SGTDFTLTIN SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S80N S67Y (PDB) S60M S76N S63Y (Kabat) 
               
               
                 Seq. ID No. 77 
                   
               
               
                 GVPMRFYGSG SGTDFTLTIN SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R S80N S67Y (PDB) S77R S76N S63Y (Kabat) 
               
               
                 Seq. ID No. 78 
                   
               
               
                 GVPSRFYGSG SGTDFTLTIN RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 Q83R S80N S67Y (PDB) Q79R S76N S63Y (Kabat) 
               
               
                 Seq. ID No. 79 
                   
               
               
                 GVPSRFYGSG SGTDFTLTIN SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R S80N S67Y (PDB) S60M S77R S76N S63Y (Kabat) 
               
               
                 Seq. ID No. 80 
                   
               
               
                 GVPMRFYGSG SGTDFTLTIN RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M Q83R S80N S67Y (PDB) S60M Q79R S76N S63Y (Kabat) 
               
               
                 Seq. ID No. 81 
                   
               
               
                 GVPMRFYGSG SGTDFTLTIN SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R Q83R S80N S67Y (PDB) S77R Q79R S76N S63Y (Kabat) 
               
               
                 Seq. ID No. 82 
                   
               
               
                 GVPSRFYGSG SGTDFTLTIN RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R Q83R S80N S67Y (PDB) S60M S77R Q79R S76N S63Y 
               
               
                 (Kabat) 
               
               
                 Seq. ID No. 83 
                   
               
               
                 GVPMRFYGSG SGTDFTLTIN RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S67Y S71M(PDB) S63Y S67M(Kabat) 
               
               
                 Seq. ID No. 84 
                   
               
               
                 GVPSRFYGSG MGTDFTLTIS SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S67Y S71M (PDB) S60M S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 85 
                   
               
               
                 GVPMRFYGSG MGTDFTLTIS SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R S67Y S71M (PDB) S77R S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 86 
                   
               
               
                 GVPSRFYGSG MGTDFTLTIS RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 Q83R S67Y S71M (PDB) Q79R S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 87 
                   
               
               
                 GVPSRFYGSG MGTDFTLTIS SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R S67Y S71M (PDB) S60M S77R S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 88 
                   
               
               
                 GVPMRFYGSG MGTDFTLTIS RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M Q83R S67Y S71M (PDB) S60M Q79R S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 89 
                   
               
               
                 GVPMRFYGSG MGTDFTLTIS SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R Q83R S67Y S71M (PDB) S77R Q79R S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 90 
                   
               
               
                 GVPSRFYGSG MGTDFTLTIS RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R Q83R S67Y S71M (PDB) S60M S77R Q79R S63Y S67M 
               
               
                 (Kabat) 
               
               
                 Seq. ID No. 91 
                   
               
               
                 GVPMRFYGSG MGTDFTLTIS RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S80N S71M (PDB) S76N S67M (Kabat) 
               
               
                 Seq. ID No. 92 
                   
               
               
                 GVPSRFSGSG MGTDFTLTIN SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S80N S71M (PDB) S60M S76N S67M (Kabat) 
               
               
                 Seq. ID No. 93 
                   
               
               
                 GVPMRFSGSG MGTDFTLTIN SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R S80N S71M (PDB) S77R S76N S67M (Kabat) 
               
               
                 Seq. ID No. 94 
                   
               
               
                 GVPSRFSGSG MGTDFTLTIN RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 Q83R S80N S71M (PDB) Q79R S76N S67M (Kabat) 
               
               
                 Seq. ID No. 95 
                   
               
               
                 GVPSRFSGSG MGTDFTLTIN SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R S80N S71M (PDB) S60M S77R S76N S67M (Kabat) 
               
               
                 Seq. ID No. 96 
                   
               
               
                 GVPMRFSGSG MGTDFTLTIN RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M Q83R S80N S71M (PDB) S60M Q79R S76N S67M (Kabat) 
               
               
                 Seq. ID No. 97 
                   
               
               
                 GVPMRFSGSG MGTDFTLTIN SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R Q83R S80N S71M (PDB) S77R Q79R S76N S67M (Kabat) 
               
               
                 Seq. ID No. 98 
                   
               
               
                 GVPSRFSGSG MGTDFTLTIN RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R Q83R S80N S71M (PDB) S60M S77R Q79R S76N S67M 
               
               
                 (Kabat) 
               
               
                 Seq. ID No. 99 
                   
               
               
                 GVPMRFSGSG MGTDFTLTIN RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S80N S67Y S71M (PDB) S76N S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 100 
                   
               
               
                 GVPSRFYGSG MGTDFTLTIN SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S80N S67Y S71M (PDB) S60M S76N S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 101 
                   
               
               
                 GVPMRFYGSG MGTDFTLTIN SLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R S80N S67Y S71M (PDB) S77R S76N S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 102 
                   
               
               
                 GVPSRFYGSG MGTDFTLTIN RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 Q83R S80N S67Y S71M (PDB) Q79R S76N S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 103 
                   
               
               
                 GVPSRFYGSG MGTDFTLTIN SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R S80N S67Y S71M (PDB) S60M S77R S76N S63Y S67M 
               
               
                 (Kabat) 
               
               
                 Seq. ID No. 104 
                   
               
               
                 GVPMRFYGSG MGTDFTLTIN RLQPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M Q83R S80N S67Y S71M (PDB) S60M Q79R S76N S63Y S67M 
               
               
                 (Kabat) 
               
               
                 Seq. ID No. 105 
                   
               
               
                 GVPMRFYGSG MGTDFTLTIN SLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S81R Q83R S80N S67Y S71M (PDB) S77R Q79R S76N S63Y S67M 
               
               
                 (Kabat) 
               
               
                 Seq. ID No. 106 
                   
               
               
                 GVPSRFYGSG MGTDFTLTIN RLRPEDFATY YC 
                   
               
               
                   
               
               
                 FR L3 S64M S81R Q83R S80N S67Y S71M (PDB) S60M S77R Q79R S76N 
               
               
                 S63Y S67M (Kabat) 
               
               
                 Seq. ID No. 107 
                   
               
               
                 GVPMRFYGSG MGTDFTLTIN RLRPEDFATY YC 
                   
               
               
                   
               
               
                 Wild-type human IgE-Fc (Ce2-Ce4 domains with numbering V224-K547 
               
               
                 according to Dorrington &amp; Bennich (1978) Immunol. Rev. 41:3-25) 
               
               
                 carrying a C225A mutation; NB, in the crystallography 
               
               
                 experiments the following mutations were also inserted into the 
               
               
                 IgE-Fc to simplify the glycosylation pattern: N265Q &amp; N371Q 
               
               
                 (again according to Dorrington &amp; Bennich numbering) 
               
               
                 Seq. ID No. 108 
                   
               
               
                 VASRDFTPPT VKILQSSCDG GGHFPPTIQL LCLVSGYTPG TINITWLEDG QVMDVDLSTA 
                   
               
               
                   
               
               
                 STTQEGELAS TQSELTLSQK HWLSDRTYTC QVTYQGHTFE DSTKKCADSN PRGVSAYLSR 
               
               
                   
               
               
                 PSPFDLFIRK SPTITCLVVD LAPSKGTVNL TWSRASGKPV NHSTRKEEKQ RNGTLTVTST 
               
               
                   
               
               
                 LPVGTRDWIE GETYQCRVTH PHLPRALMRS TTKTSGPRAA PEVYAFATPE WPGSRDKRTL 
               
               
                   
               
               
                 ACLIQNFMPE DISVQWLHNE VQLPDARHST TQPRKTKGSG FFVFSRLEVT RAEWEQKDEF 
               
               
                   
               
               
                 ICRAVHEAAS PSQTVQRAVS VNPGK 
               
               
                   
               
               
                 LIGHT CHAIN 
               
               
                 Fab1 
               
               
                 S77R_Q79R_v-region (Fab1) 
               
               
                 Seq. ID No. 109 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAASYLES GVPSRFSGSG SGTDFTLTIS RLRPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 S77R_Q79R_v-region (Fab1) 
               
               
                 Seq. ID No. 110 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAC CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCCTA CCTTGAAAGC GGCGTGCCTT CACGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCTGGCACCG ATTTCACCCT GACCATTTCG AGACTGAGGC 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGTACCAA GGTCGAAATC AAG 
               
               
                   
               
               
                 S77R_Q79R_v-region (Fab1) with signal sequence underlined and 
               
               
                 italicised 
               
               
                 Seq. ID No. 111 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PSRFSGSGSG TDFTLTISRL 
               
               
                   
               
               
                 RPEDFATYYC QQSHEDPYTF GQGTKVEIK 
               
               
                   
               
               
                 S77R_Q79R_v-region (Fab1) with signal sequence underlined and 
               
               
                 italicised 
               
               
                 Seq. ID No. 112 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTACCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CCTACCTTGA AAGCGGCGTG CCTTCACGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCTGGC ACCGATTTCA CCCTGACCAT TTCGAGACTG 
               
               
                   
               
               
                 AGGCCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGTA CCAAGGTCGA AATCAAG 
               
               
                   
               
               
                 Fab2 
               
               
                 Omalizumab v-region + kappa constant region including L154P 
               
               
                 Fab2_v-region 
               
               
                 Seq. ID No. 113 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAASYLES GVPSRFSGSG SGTDFTLTIS SLQPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 Fab2_v-region 
               
               
                 Seq. ID No. 114 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAT CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCATA CCTTGAAAGC GGCGTGCCT T   CG CGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCGGGCACCG ATTTCACCCT GACCATTTCG T CG CTG CAG C 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGAACCAA GGTCGAAATC AAG 
               
               
                   
               
               
                 Fab2_v-region with signal sequence underlined and italicised 
               
               
                 Seq. ID No. 115 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PSRFSGSGSG TDFTLTISSL 
               
               
                   
               
               
                 QPEDFATYYC QQSHEDPYTF GQGTKVEIK 
               
               
                   
               
               
                 Fab2_v-region with signal 
               
               
                 sequence underlined and italicised 
               
               
                 Seq. ID No. 116 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTATCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CATACCTTGA AAGCGGCGTG CCTTCGCGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCGGGC ACCGATTTCA CCCTGACCAT TTCGTCGCTG 
               
               
                   
               
               
                 CAGCCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGAA CCAAGGTCGA AATCAAG 
               
               
                   
               
               
                 Fab2_v-region + kappa constant region including L154P 
               
               
                 Seq. ID No. 117 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAASYLES GVPSRFSGSG SGTDFTLTIS SLQPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI KRTVAAPSVF IFPPSDEQLK SGTASVVCLL NNFYPREAKV 
               
               
                   
               
               
                 QWKVDNAPQS GNSQESVTEQ DSKDSTYSLS STLTLSKADY EKHKVYACEV 
               
               
                   
               
               
                 THQGLSSPVT KSFNRGEC 
               
               
                   
               
               
                 Fab2_v-region + kappa constant region including L154P 
               
               
                 Seq. ID No. 118 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAT CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCATA CCTTGAAAGC GGCGTGCCT T   CG CGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCGGGCACCG ATTTCACCCT GACCATTTCG  TCG CTG CAG C 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGAACCAA GGTCGAAATC AAGCGTACGG TAGCGGCCCC 
               
               
                   
               
               
                 ATCTGTCTTC ATCTTCCCGC CATCTGATGA GCAGTTGAAA TCTGGAACTG 
               
               
                   
               
               
                 CCTCTGTTGT GTGCCTGCTG AATAACTTCT ATCCCAGAGA GGCCAAAGTA 
               
               
                   
               
               
                 CAGTGGAAGG TGGATAACGC CCCGCAATCG GGTAACTCCC AGGAGAGTGT 
               
               
                   
               
               
                 CACAGAGCAG GACAGCAAGG ACAGCACCTA CAGCCTCAGC AGCACCCTGA 
               
               
                   
               
               
                 CGCTGAGCAA AGCAGACTAC GAGAAACACA AAGTCTACGC CTGCGAAGTC 
               
               
                   
               
               
                 ACCCATCAGG GCCTGAGCTC GCCCGTCACA AAGAGCTTCA ACAGGGGAGA 
               
               
                   
               
               
                 GTGT 
               
               
                   
               
               
                 Fab2 _v-region + kappa constant region including L154P with 
               
               
                 signal sequence underlined and italicised 
               
               
                 Seq. ID No. 119 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PSRFSGSGSG TDFTLTISSL 
               
               
                   
               
               
                 QPEDFATYYC QQSHEDPYTF GQGTKVEIKR TVAAPSVFIF PPSDEQLKSG 
               
               
                   
               
               
                 TASVVCLLNN FYPREAKVQW KVDNAPQSGN SQESVTEQDS KDSTYSLSST 
               
               
                   
               
               
                 LTLSKADYEK HKVYACEVTH QGLSSPVTKS FNRGEC 
               
               
                   
               
               
                 Fab2 _v-region + kappa constant region including L154P with 
               
               
                 signal sequence underlined and italicised 
               
               
                 Seq. ID No. 120 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTATCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CATACCTTGA AAGCGGCGTG CCT TCG CGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCGGGC ACCGATTTCA CCCTGACCAT TTCG TCG CTG 
               
               
                   
               
               
                   CAG CCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGAA CCAAGGTCGA AATCAAGCGT ACGGTAGCGG 
               
               
                   
               
               
                 CCCCATCTGT CTTCATCTTC CCGCCATCTG ATGAGCAGTT GAAATCTGGA 
               
               
                   
               
               
                 ACTGCCTCTG TTGTGTGCCT GCTGAATAAC TTCTATCCCA GAGAGGCCAA 
               
               
                   
               
               
                 AGTACAGTGG AAGGTGGATA ACGCCCCGCA ATCGGGTAAC TCCCAGGAGA 
               
               
                   
               
               
                 GTGTCACAGA GCAGGACAGC AAGGACAGCA CCTACAGCCT CAGCAGCACC 
               
               
                   
               
               
                 CTGACGCTGA GCAAAGCAGA CTACGAGAAA CACAAAGTCT ACGCCTGCGA 
               
               
                   
               
               
                 AGTCACCCAT CAGGGCCTGA GCTCGCCCGT CACAAAGAGC TTCAACAGGG 
               
               
                   
               
               
                 GAGAGTGT 
               
               
                   
               
               
                 Fab3 
               
               
                 S77R_Q79R_v-region (from Fab1) + kappa constant region 
               
               
                 including L154P (from Fab2) 
               
               
                 S77R_Q79R_v-region 
               
               
                 Seq. ID No. 121 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAASYLES GVPSRFSGSG SGTDFTLTIS RLRPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 S77R_Q79R_v-region 
               
               
                 Seq. ID No. 122 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAC CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCCTA CCTTGAAAGC GGCGTGCCTT CACGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCTGGCACCG ATTTCACCCT GACCATTTCG AGACTGAGGC 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGTACCAA GGTCGAAATC AAG 
               
               
                   
               
               
                 S77R_Q79R_v-region with signal sequence underlined and 
               
               
                 italicised 
               
               
                 Seq. ID No. 123 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PSRFSGSGSG TDFTLTISRL 
               
               
                   
               
               
                 RPEDFATYYC QQSHEDPYTF GQGTKVEIK 
               
               
                   
               
               
                 S77R_Q79R_v-region with signal sequence underlined and 
               
               
                 italicised 
               
               
                 Seq. ID No. 124 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTACCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CCTACCTTGA AAGCGGCGTG CCTTCACGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCTGGC ACCGATTTCA CCCTGACCAT TTCGAGACTG 
               
               
                   
               
               
                 AGGCCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGTA CCAAGGTCGA AATCAAG 
               
               
                   
               
               
                 S77R_Q79R_v-region + kappa constant region including 
               
               
                 L154P 
               
               
                 Seq. ID No. 125 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAASYLES GVPSRFSGSG SGTDFTLTIS RLRPEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI KRTVAAPSVF IFPPSDEQLK SGTASVVCLL NNFYPREAKV 
               
               
                   
               
               
                 QWKVDNAPQS GNSQESVTEQ DSKDSTYSLS STLTLSKADY EKHKVYACEV 
               
               
                   
               
               
                 THQGLSSPVT KSFNRGEC 
               
               
                   
               
               
                 S77R_Q79R_v-region + kappa constant region including 
               
               
                 L154P 
               
               
                 Seq. ID No. 126 
                   
               
               
                 GATATTCAGC TGACTCAGAG CCCGAGCTCA CTCTCCGCTT CCGTGGGGGA 
                   
               
               
                   
               
               
                 TAGAGTGACC ATCACTTGCC GGGCATCCCA GTCGGTGGAC TACGACGGAG 
               
               
                   
               
               
                 ACTCCTACAT GAACTGGTAC CAGCAGAAGC CCGGAAAAGC CCCAAAGTTG 
               
               
                   
               
               
                 CTGATCTACG CCGCCTCCTA CCTTGAAAGC GGCGTGCCTT CACGCTTCTC 
               
               
                   
               
               
                 GGGAAGCGGG TCTGGCACCG ATTTCACCCT GACCATTTCG AGACTGAGGC 
               
               
                   
               
               
                 CGGAGGACTT CGCGACTTAC TACTGCCAAC AGTCCCACGA GGACCCCTAT 
               
               
                   
               
               
                 ACGTTTGGCC AGGGTACCAA GGTCGAAATC AAGCGTACGG TAGCGGCCCC 
               
               
                   
               
               
                 ATCTGTCTTC ATCTTCCCGC CATCTGATGA GCAGTTGAAA TCTGGAACTG 
               
               
                   
               
               
                 CCTCTGTTGT GTGCCTGCTG AATAACTTCT ATCCCAGAGA GGCCAAAGTA 
               
               
                   
               
               
                 CAGTGGAAGG TGGATAACGC CCCGCAATCG GGTAACTCCC AGGAGAGTGT 
               
               
                   
               
               
                 CACAGAGCAG GACAGCAAGG ACAGCACCTA CAGCCTCAGC AGCACCCTGA 
               
               
                   
               
               
                 CGCTGAGCAA AGCAGACTAC GAGAAACACA AAGTCTACGC CTGCGAAGTC 
               
               
                   
               
               
                 ACCCATCAGG GCCTGAGCTC GCCCGTCACA AAGAGCTTCA ACAGGGGAGA 
               
               
                   
               
               
                 GTGT 
               
               
                   
               
               
                 S77R_Q79R_v-region + kappa constant region including 
               
               
                 L154P with signal sequence underlined and italicised 
               
               
                 Seq. ID No. 127 
                   
               
               
                        QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAASYLESGV PSRFSGSGSG TDFTLTISRL 
               
               
                   
               
               
                 RPEDFATYYC QQSHEDPYTF GQGTKVEIKR TVAAPSVFIF PPSDEQLKSG 
               
               
                   
               
               
                 TASVVCLLNN FYPREAKVQW KVDNAPQSGN SQESVTEQDS KDSTYSLSST 
               
               
                   
               
               
                 LTLSKADYEK HKVYACEVTH QGLSSPVTKS FNRGEC 
               
               
                   
               
               
                 S77R_Q79R_v-region + kappa constant region including 
               
               
                 L154P with signal sequence underlined and italicised 
               
               
                 Seq. ID No. 128 
                   
               
               
                                 
                   
               
               
                   
               
               
                    GATATT CAGCTGACTC AGAGCCCGAG CTCACTCTCC GCTTCCGTGG 
               
               
                   
               
               
                 GGGATAGAGT GACCATCACT TGCCGGGCAT CCCAGTCGGT GGACTACGAC 
               
               
                   
               
               
                 GGAGACTCCT ACATGAACTG GTACCAGCAG AAGCCCGGAA AAGCCCCAAA 
               
               
                   
               
               
                 GTTGCTGATC TACGCCGCCT CCTACCTTGA AAGCGGCGTG CCTTCACGCT 
               
               
                   
               
               
                 TCTCGGGAAG CGGGTCTGGC ACCGATTTCA CCCTGACCAT TTCGAGACTG 
               
               
                   
               
               
                 AGGCCGGAGG ACTTCGCGAC TTACTACTGC CAACAGTCCC ACGAGGACCC 
               
               
                   
               
               
                 CTATACGTTT GGCCAGGGTA CCAAGGTCGA AATCAAGCGT ACGGTAGCGG 
               
               
                   
               
               
                 CCCCATCTGT CTTCATCTTC CCGCCATCTG ATGAGCAGTT GAAATCTGGA 
               
               
                   
               
               
                 ACTGCCTCTG TTGTGTGCCT GCTGAATAAC TTCTATCCCA GAGAGGCCAA 
               
               
                   
               
               
                 AGTACAGTGG AAGGTGGATA ACGCCCCGCA ATCGGGTAAC TCCCAGGAGA 
               
               
                   
               
               
                 GTGTCACAGA GCAGGACAGC AAGGACAGCA CCTACAGCCT CAGCAGCACC 
               
               
                   
               
               
                 CTGACGCTGA GCAAAGCAGA CTACGAGAAA CACAAAGTCT ACGCCTGCGA 
               
               
                   
               
               
                 AGTCACCCAT CAGGGCCTGA GCTCGCCCGT CACAAAGAGC TTCAACAGGG 
               
               
                   
               
               
                 GAGAGTGT 
               
               
                   
               
               
                 Omalizumab_v-region 
               
               
                 Seq. ID No. 129 
                   
               
               
                 TQSPSSLSAS VGDRVTITCR ASQSVDYDGD SYMNWYQQKP GKAPKLLIYA 
                   
               
               
                   
               
               
                 ASYLESGVPS RFSGSGSGTD FTLTISSLQP EDFATYYCQQ SHEDPYTFGQ 
               
               
                   
               
               
                 GTKVEIK 
               
               
                   
               
               
                 Omalizumab_v-region 
               
               
                 Seq. ID No. 130 
                   
               
               
                 ACTCAGAGCC CGAGCTCACT CTCCGCTTCC GTGGGGGATA GAGTGACCAT 
                   
               
               
                   
               
               
                 CACTTGCCGG GCATCCCAGT CGGTGGACTA CGACGGAGAC TCCTACATGA 
               
               
                   
               
               
                 ACTGGTATCA GCAGAAGCCC GGAAAAGCCC CAAAGTTGCT GATCTACGCC 
               
               
                   
               
               
                 GCCTCATACC TTGAAAGCGG CGTGCCT TCG  CGCTTCTCGG GAAGCGGGTC 
               
               
                   
               
               
                 GGGCACCGAT TTCACCCTGA CCATTTCG TC G CTG CAG CCG GAGGACTTCG 
               
               
                   
               
               
                 CGACTTACTA CTGCCAACAG TCCCACGAGG ACCCCTATAC GTTTGGCCAG 
               
               
                   
               
               
                 GGAACCAAGG TCGAAATCAA G 
               
               
                   
               
               
                 FR3 
               
               
                 SEQ ID NO: 131 
                   
               
               
                 GVPMRFSGSGMGTDFTLTISRLRPEDFATYYC 
                   
               
               
                   
               
               
                 S60M_552D_667M S77R_Q79R_v-region 
               
               
                 SEQ ID NO: 132 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAA D YLES GVP M RFSGSG  M GTDFTLTIS  R L R PEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 S60M_552D_667M_S77R_Q79R_v-region 
               
               
                 SEQ ID NO: 133 
                   
               
               
                 GATATTCAGCTGACTCAGAGCCCGAGCTCACTCTCCGCTTCCGTGGGGGATAGAGTGACCATCA 
                   
               
               
                   
               
               
                 CTTGCCGGGCATCCCAGTCGGTGGACTACGACGGAGACTCCTACATGAACTGGTATCAGCAGAA 
               
               
                   
               
               
                 GCCCGGAAAAGCCCCAAAGTTGCTGATCTACGCCGCCGACTACCTTGAAAGCGGCGTGCCTATG 
               
               
                   
               
               
                 CGCTTCTCGGGAAGCGGGATGGGCACCGATTTCACCCTGACCATTTCGAGACTGAGGCCGGAGG 
               
               
                   
               
               
                 ACTTCGCGACTTACTACTGCCAACAGTCCCACGAGGACCCCTATACGTTTGGCCAGGGAACCAA 
               
               
                   
               
               
                 GGTCGAAATCAAG 
               
               
                   
               
               
                 S60M_S52D_S67M_S77R_Q79R_v-region with signal 
               
               
                 sequence underlined and italicised 
               
               
                 SEQ ID NO: 134 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAA D YLESGV P M RFSGSG M G TDFTLTIS R L 
               
               
                   
               
               
                   R PEDFATYYC QQSHEDPYTF GQGTKVEIK 
               
               
                   
               
               
                 S60M_S52D_S67M_S77R_Q79R_v-region with signal 
               
               
                 sequence underlined and italicised 
               
               
                 SEQ ID NO: 135 
                   
               
               
                 ATGAAGTGGGTCACCTTCATCTCCCTGCTGTTTCTGTTCTCCTCCGCCTACTCCGATATTCAGC 
                   
               
               
                   
               
               
                 TGACTCAGAGCCCGAGCTCACTCTCCGCTTCCGTGGGGGATAGAGTGACCATCACTTGCCGGGC 
               
               
                   
               
               
                 ATCCCAGTCGGTGGACTACGACGGAGACTCCTACATGAACTGGTATCAGCAGAAGCCCGGAAAA 
               
               
                   
               
               
                 GCCCCAAAGTTGCTGATCTACGCCGCCGACTACCTTGAAAGCGGCGTGCCTATGCGCTTCTCGG 
               
               
                   
               
               
                 GAAGCGGGATGGGCACCGATTTCACCCTGACCATTTCGAGACTGAGGCCGGAGGACTTCGCGAC 
               
               
                   
               
               
                 TTACTACTGCCAACAGTCCCACGAGGACCCCTATACGTTTGGCCAGGGAACCAAGGTCGAAATC 
               
               
                   
               
               
                 AAG 
               
               
                   
               
               
                 S60M_S52D_667M_S77R_Q79R_v-region + kappa constant region 
               
               
                 SEQ ID NO: 136 
                   
               
               
                 DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD GDSYMNWYQQ KPGKAPKLLI 
                   
               
               
                   
               
               
                 YAA D YLESGV P M RFSGSG M G TDFTLTIS R L  R PEDFATYYC QQSHEDPYTF 
               
               
                   
               
               
                 GQGTKVEIKR TVAAPSVFIF PPSDEQLKSG TASVVCLLNN FYPREAKVQW 
               
               
                   
               
               
                 KVDNALQSGN SQESVTEQDS KDSTYSLSST LTLSKADYEK HKVYACEVTH 
               
               
                   
               
               
                 QGLSSPVTKS FNRGEC 
               
               
                   
               
               
                 S60M_S52D_667M_S77R_Q79R_v-region + kappa constant region 
               
               
                 SEQ ID NO: 137 
                   
               
               
                 GATATTCAGCTGACTCAGAGCCCGAGCTCACTCTCCGCTTCCGTGGGGGATAGAGTGACCATCA 
                   
               
               
                   
               
               
                 CTTGCCGGGCATCCCAGTCGGTGGACTACGACGGAGACTCCTACATGAACTGGTATCAGCAGAA 
               
               
                   
               
               
                 GCCCGGAAAAGCCCCAAAGTTGCTGATCTACGCCGCCGACTACCTTGAAAGCGGCGTGCCTATG 
               
               
                   
               
               
                 CGCTTCTCGGGAAGCGGGATGGGCACCGATTTCACCCTGACCATTTCGAGACTGAGGCCGGAGG 
               
               
                   
               
               
                 ACTTCGCGACTTACTACTGCCAACAGTCCCACGAGGACCCCTATACGTTTGGCCAGGGAACCAA 
               
               
                   
               
               
                 GGTCGAAATCAAGCGTACGGTAGCGGCCCCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAG 
               
               
                   
               
               
                 TTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAG 
               
               
                   
               
               
                 TACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGA 
               
               
                   
               
               
                 CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA 
               
               
                   
               
               
                 CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCA 
               
               
                   
               
               
                 ACAGGGGAGAGTGT 
               
               
                   
               
               
                 FR3 
               
               
                 SEQ ID NO: 138 
                   
               
               
                 GVP R RFSGSG M GTDFTLTIS R L R PEDFATYYC 
                   
               
               
                   
               
               
                 S60R_S52D_S67M_S77R_Q79R_v-region 
               
               
                 SEQ ID NO: 139 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAA D YLES GVP R RFSGSG  M GTDFTLTIS  R L R PEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 S60R_S52D_667M_S77R_Q79R_v-region 
               
               
                 SEQ ID NO: 140 
                   
               
               
                 GATATTCAGCTGACTCAGAGCCCGAGCTCACTCTCCGCTTCCGTGGGGGATAGAGTGACCATCA 
                   
               
               
                   
               
               
                 CTTGCCGGGCATCCCAGTCGGTGGACTACGACGGAGACTCCTACATGAACTGGTATCAGCAGAA 
               
               
                   
               
               
                 GCCCGGAAAAGCCCCAAAGTTGCTGATCTACGCCGCCGATTACCTTGAAAGCGGCGTGCCTCGT 
               
               
                   
               
               
                 CGCTTCTCGGGAAGCGGGATGGGCACCGATTTCACCCTGACCATTTCGAGACTGAGGCCGGAGG 
               
               
                   
               
               
                 ACTTCGCGACTTACTACTGCCAACAGTCCCACGAGGACCCCTATACGTTTGGCCAGGGAACCAA 
               
               
                   
               
               
                 GGTCGAAATCAAG 
               
               
                   
               
               
                 S60R_S52D_S67M_S77R_Q79R_v-region with signal 
               
               
                 sequence underlined and italicised 
               
               
                 SEQ ID NO: 141 
                   
               
               
                       DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD 
                   
               
               
                   
               
               
                 GDSYMNWYQQ KPGKAPKLLI YAA D YLESGV P R RFSGSG M G TDFTLTIS R L 
               
               
                   
               
               
                   R PEDFATYYC QQSHEDPYTF GQGTKVEIK 
               
               
                   
               
               
                 S60R_S52D_S67M_S77R_Q79R_v-region with signal 
               
               
                 sequence underlined and italicised 
               
               
                 SEQ ID NO: 142 
                   
               
               
                 ATGAAGTGGGTCACCTTCATCTCCCTGCTGTTTCTGTTCTCCTCCGCCTACTCCGATATTCAGC 
                   
               
               
                   
               
               
                 TGACTCAGAGCCCGAGCTCACTCTCCGCTTCCGTGGGGGATAGAGTGACCATCACTTGCCGGGC 
               
               
                   
               
               
                 ATCCCAGTCGGTGGACTACGACGGAGACTCCTACATGAACTGGTATCAGCAGAAGCCCGGAAAA 
               
               
                   
               
               
                 GCCCCAAAGTTGCTGATCTACGCCGCCGATTACCTTGAAAGCGGCGTGCCTCGTCGCTTCTCGG 
               
               
                   
               
               
                 GAAGCGGGATGGGCACCGATTTCACCCTGACCATTTCGAGACTGAGGCCGGAGGACTTCGCGAC 
               
               
                   
               
               
                 TTACTACTGCCAACAGTCCCACGAGGACCCCTATACGTTTGGCCAGGGAACCAAGGTCGAAATC 
               
               
                   
               
               
                 AAG 
               
               
                   
               
               
                 S60R_552D_667M_S77R_Q79R_v-region + kappa constant region 
               
               
                 SEQ ID NO: 143 
                   
               
               
                 DI QLTQSPSSLS ASVGDRVTIT CRASQSVDYD GDSYMNWYQQ KPGKAPKLLI 
                   
               
               
                   
               
               
                 YAA D YLESGV P R RFSGSG M G TDFTLTIS R L  R PEDFATYYC QQSHEDPYTF 
               
               
                   
               
               
                 GQGTKVEIKR TVAAPSVFIF PPSDEQLKSG TASVVCLLNN FYPREAKVQW 
               
               
                   
               
               
                 KVDNALQSGN SQESVTEQDS KDSTYSLSST LTLSKADYEK HKVYACEVTH 
               
               
                   
               
               
                 QGLSSPVTKS FNRGEC 
               
               
                   
               
               
                 S60R_552D_667M_S77R_Q79R_v-region + kappa constant region 
               
               
                 SEQ ID NO: 144 
                   
               
               
                 GATATTCAGCTGACTCAGAGCCCGAGCTCACTCTCCGCTTCCGTGGGGGATAGAGTGACCATCA 
                   
               
               
                   
               
               
                 CTTGCCGGGCATCCCAGTCGGTGGACTACGACGGAGACTCCTACATGAACTGGTATCAGCAGAA 
               
               
                   
               
               
                 GCCCGGAAAAGCCCCAAAGTTGCTGATCTACGCCGCCGATTACCTTGAAAGCGGCGTGCCTCGT 
               
               
                   
               
               
                 CGCTTCTCGGGAAGCGGGATGGGCACCGATTTCACCCTGACCATTTCGAGACTGAGGCCGGAGG 
               
               
                   
               
               
                 ACTTCGCGACTTACTACTGCCAACAGTCCCACGAGGACCCCTATACGTTTGGCCAGGGAACCAA 
               
               
                   
               
               
                 GGTCGAAATCAAGCGTACGGTAGCGGCCCCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAG 
               
               
                   
               
               
                 TTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAG 
               
               
                   
               
               
                 TACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGA 
               
               
                   
               
               
                 CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA 
               
               
                   
               
               
                 CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCA 
               
               
                   
               
               
                 ACAGGGGAGAGTGT 
               
               
                   
               
               
                 S60K_552D_S67M_S77R_Q79R_v-region(Kabat) 
               
               
                 SEQ ID NO: 145 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAA D YLES GVP K RFSGSG  M GTDFTLTIS  R L R PEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 S60Q_552D_S67M S77R_Q79R_v-region(Kabat) 
               
               
                 SEQ ID NO: 146 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAA D YLES GVP Q RFSGSG  M GTDFTLTIS  R L R PEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 v-region + gamma 1 CH1 constant region plus linker plus CA645 
               
               
                 gL4gH5 scFv with signal sequence underlined and italicised 
               
               
                 SEQ ID NO: 147 
                   
               
               
                    EVQLVESGGGLVQPGGSLRLSCAVSGYSITSGYSWNWIRQAPGKGL 
                   
               
               
                   
               
               
                 EWVASITYDGSTNYNPSVKGRITISRDDSKNTFYLQMNSLRAEDTAVYYCARGSHYFGHWHFAV 
               
               
                   
               
               
                 WGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTF 
               
               
                   
               
               
                 PAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCSGGGGTGGGGSEVQ 
               
               
                   
               
               
                 LLESGGGLVQPGGSLRLSCAVSGIDLSNYAINWVRQAPGKCLEWIGIIWASGTTFYATWAKGRF 
               
               
                   
               
               
                 TISRDNSKNTVYLQMNSLRAEDTAVYYCARTVPGYSTAPYFDLWGQGTLVTVSSGGGGSGGGGS 
               
               
                   
               
               
                 GGGGSGGGGSDIQMTQSPSSVSASVGDRVTITCQSSPSVWSNFLSWYQQKPGKAPKLLIYEASK 
               
               
                   
               
               
                 LTSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCGGGYSSISDTTFGCGTKVEIKRT 
               
               
                   
               
               
                 v-region + gamma 1 CH1 constant region plus linker plus CA645 
               
               
                 gL4gH5 scFv with signal sequence underlined and italicised 
               
               
                 SEQ ID NO: 148 
                   
               
               
                      GAAGTGCAGT 
                   
               
               
                   
               
               
                 TGGTGGAGTCGGGTGGAGGGCTGGTGCAGCCTGGCGGTAGCCTGAGGCTGTCCTGTGCCGTGTC 
               
               
                   
               
               
                 CGGATACTCCATTACCTCCGGCTACTCGTGGAACTGGATCAGACAGGCTCCCGGAAAGGGACTT 
               
               
                   
               
               
                 GAGTGGGTGGCGTCCATCACCTACGACGGCTCAACCAACTATAACCCGTCCGTGAAGGGCCGCA 
               
               
                   
               
               
                 TCACCATTTCGCGCGACGACAGCAAGAATACTTTTTACCTCCAAATGAACAGCCTGCGGGCCGA 
               
               
                   
               
               
                 AGATACTGCCGTGTACTACTGCGCGCGGGGATCACATTACTTCGGGCACTGGCACTTCGCCGTC 
               
               
                   
               
               
                 TGGGGACAGGGCACCCTCGTCACTGTCTCGAGCGCTTCTACAAAGGGCCCATCGGTCTTCCCCC 
               
               
                   
               
               
                 TGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA 
               
               
                   
               
               
                 CTTCCCCGAACCAGTGACGGTGTCGTGGAACTCAGGTGCCCTGACCAGCGGCGTTCACACCTTC 
               
               
                   
               
               
                 CCGGCTGTCCTACAGTCTTCAGGACTCTACTCCCTGAGCAGCGTGGTGACCGTGCCCTCCAGCA 
               
               
                   
               
               
                 GCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTCGATAA 
               
               
                   
               
               
                 GAAAGTTGAGCCCAAATCTTGTAGTGGAGGTGGGGGCACCGGTGGAGGTGGCAGCGAGGTTCAA 
               
               
                   
               
               
                 CTGCTTGAGTCTGGAGGAGGCCTAGTCCAGCCTGGAGGGAGCCTGCGTCTCTCTTGTGCAGTAA 
               
               
                   
               
               
                 GCGGCATCGACCTGAGCAATTACGCCATCAACTGGGTGAGACAAGCTCCGGGGAAGTGTTTAGA 
               
               
                   
               
               
                 ATGGATCGGTATAATATGGGCCAGTGGGACGACCTTTTATGCTACATGGGCGAAAGGAAGGTTT 
               
               
                   
               
               
                 ACAATTAGCCGGGACAATAGCAAAAACACCGTGTATCTCCAAATGAACTCCTTGCGAGCAGAGG 
               
               
                   
               
               
                 ACACGGCGGTGTACTATTGTGCTCGCACTGTCCCAGGTTATAGCACTGCACCCTACTTCGATCT 
               
               
                   
               
               
                 GTGGGGACAAGGGACCCTGGTGACTGTTTCAAGTGGCGGAGGGGGTAGTGGAGGGGGTGGCTCT 
               
               
                   
               
               
                 GGGGGTGGCGGAAGCGGTGGCGGGGGTTCTGACATACAAATGACTCAGTCTCCTTCATCGGTAT 
               
               
                   
               
               
                 CCGCGTCCGTTGGCGATAGGGTGACTATTACATGTCAAAGCTCTCCTAGCGTCTGGAGCAATTT 
               
               
                   
               
               
                 TCTATCCTGGTATCAACAGAAACCGGGGAAGGCTCCAAAACTTCTGATTTATGAAGCCTCGAAA 
               
               
                   
               
               
                 CTCACCAGTGGAGTTCCGTCAAGATTCAGTGGCTCTGGATCAGGGACAGACTTCACGTTGACAA 
               
               
                   
               
               
                 TCAGTTCGCTGCAACCAGAGGACTTTGCGACCTACTATTGTGGTGGAGGTTACAGTAGCATAAG 
               
               
                   
               
               
                 TGATACGACATTTGGGTGCGGTACTAAGGTGGAAATCAAACGTACC 
               
               
                   
               
               
                 Linker between CH1 and CA645 gL4gH5 scnr 
               
               
                 SEQ ID NO: 149 
                   
               
               
                 SGGGGTGGGGS 
                   
               
               
                   
               
               
                 CA645 gL4gH5 scnr 
               
               
                 SEQ ID NO: 150 
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAVSGIDLSNYAINWVRQAPGKCLEWIGIIWASGTTFYATWAK 
                   
               
               
                   
               
               
                 GRFTISRDNSKNTVYLQMNSLRAEDTAVYYCARTVPGYSTAPYFDLWGQGTLVTVSSGGGGSGG 
               
               
                   
               
               
                 GGSGGGGSGGGGSDIQMTQSPSSVSASVGDRVTITCQSSPSVWSNFLSWYQQKPGKAPKLLIYE 
               
               
                   
               
               
                 ASKLTSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCGGGYSSISDTTFGCGTKVEIKRT 
               
               
                   
               
               
                 Linker between CA645 gH5 and CA645 gL4 within scnr 
               
               
                 SEQ ID NO: 151 
                   
               
               
                 GGGGSGGGGSGGGGSGGGGS 
                   
               
               
                   
               
               
                 CA645 CDRH1 
               
               
                 SEQ ID NO: 152 
                   
               
               
                 GIDLSNYAIN 
                   
               
               
                   
               
               
                 CA645 CDRH2 
               
               
                 SEQ ID NO: 153 
                   
               
               
                 IIWASGTTFYATWAKG 
                   
               
               
                   
               
               
                 CA645 CDRH3 
               
               
                 SEQ ID NO: 154 
                   
               
               
                 TVPGYSTAPYFDL 
                   
               
               
                   
               
               
                 CA645 CDRL1 
               
               
                 SEQ ID NO: 155 
                   
               
               
                 QSSPSVWSNFLS 
                   
               
               
                   
               
               
                 CA645 CDRL2 
               
               
                 SEQ ID NO: 156 
                   
               
               
                 EASKLTS 
                   
               
               
                   
               
               
                 CA645 CDRL3 
               
               
                 SEQ ID NO: 157 
                   
               
               
                 GGGYSSISDTT 
                   
               
               
                   
               
               
                 S60M S52D_667M S77R_Q79R_S63W_S76N_v-region(Kabat) 
               
               
                 SEQ ID NO: 158 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAA D YLES GVP M RF W GSG  M GTDFTLTI N   R L R PEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 S60M S52D_667M S77R_Q79R_S63Y_676N_v-region(Kabat) 
               
               
                 SEQ ID NO: 159 
                   
               
               
                 DIQLTQSPSS LSASVGDRVT ITCRASQSVD YDGDSYMNWY QQKPGKAPKL 
                   
               
               
                   
               
               
                 LIYAADYLES GVP M RF Y GSG  M GTDFTLTI N   R L R PEDFATY YCQQSHEDPY 
               
               
                   
               
               
                 TFGQGTKVEI K 
               
               
                   
               
               
                 Signal sequence 
               
               
                 SEQ ID NO: 160 
                   
               
               
                 MKWVTFISLL FLFSSAYS