Patent Publication Number: US-2023135471-A1

Title: Ubiquitin variants with improved affinity for 53bp1

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application claims benefit of priority under 35 U.S.C. 119 to U.S. Provisional Patent Application Ser. No. 63/248,300, filed Sep. 24, 2021, U.S. Provisional Patent Application Ser. No. 63/278,155, filed Nov. 11, 2021, and U.S. Provisional Patent Application Ser. No. 63/321,384, filed Mar. 18, 2022, wherein each application is entitled “UBIQUITIN VARIANTS WITH IMPROVED AFFINITY FOR 53BP1,” the contents of each application are herein incorporated by reference in its entirety. 
    
    
     SEQUENCE LISTING 
     The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on ______, is named IDT01-021-US_ST25.xml, and is ______ bytes in size. 
     FIELD OF THE INVENTION 
     This invention pertains to ubiquitin polypeptide variants with increased affinity for 53BP1 and improved efficacy for enhancing homology directed repair rates. 
     BACKGROUND OF THE INVENTION 
     Double-strand breaks (DSBs) of DNA are predominantly repaired through two mechanisms, non-homologous end joining (NHEJ), in which broken ends are rejoined, often imprecisely, or homology directed repair (HDR), which typically involves a sister chromatid or homologous chromosome being used as a repair template. HDR is facilitated by the presence of a sister chromatid and there are cellular mechanisms in place biasing repair towards NHEJ during the G1 phase of the cell cycle [1]. A key determinant of repair pathway choice is 53BP1. 53BP1 was first described as a binding partner of the tumor suppressor gene p53 and was later shown to be a key protein in NHEJ [2]. 53BP1 rapidly accumulates at sites of double-strand breaks. In G1, 53BP1 recruits RIF1 and inhibits end resection [3, 4]. End resection is a critical step in repair pathway choice, as it is necessary for HDR and inhibits NHEJ [1]. By inhibiting end resection, 53BP1 biases repair towards NEHJ and consequently loss of 53BP1 results in increased HDR [5]. Targeted nucleases can be introduced into cells in conjunction with a DNA repair template with homology to a targeted cut site to facilitate precise genome editing via HDR[6]. A strong inhibitor of 53BP1 is therefore useful for precise genome editing. 
     The recruitment of 53BP1 to DSB sites is dependent upon both H4K20 methylation and H2AK15 ubiquitination. 53BP1 has tandem Tudor domains that have been shown to specifically bind mono and dimethylated H4K20 and H4K20 methylation was shown to be important for 53BP1 recruitment to double-strand breaks [7, 8]. Introducing D1521R, a mutation that disrupts the activity of the Tudor domain, impairs the ability of 53BP1 to form ionizing radiation-induced foci [9]. The minimal focus-forming region of 53BP1 consists of the Tudor domain flanked by an N-terminal oligomerization region and a C-terminal extension. Notably, 53BP1 accumulation at DSBs requires the E3 ubiquitin ligase RNF168, that mediates H2AK13 and H2AK15 ubiquitination [10]. The C-terminal extension was shown to contain a ubiquitination-dependent recruitment motif (UDR) that binds specifically to H2AK15ub and is required for 53BP1 recruitment to DSB sites [9]. 
     Thus, the ubiquitin polypeptide (SEQ ID NO:1) and its interaction with 53BP1 influences the repair pathway choice for DSB sites. 
     Due to the affinity of 53BP1 for ubiquitinated H2A, a screen of ubiquitin polypeptide variants for interaction with 53BP1 was conducted recently by Canny et al. in which they discovered and modified a ubiquitin polypeptide variant with selective binding to 53BP1 that they named i53 (inhibitor of 53BP1; SEQ ID NO: 2) [11]. The top five hits from the ubiquitin polypeptide variant screen were A10, A11, C08, G08, and H04, with G08 having the highest affinity. In contrast to what might be expected, the interaction of 53BP1 with G08 did not require the UDR and the interaction was shown to be between G08 and the 53BP1 Tudor domain. To generate i53, G08 was modified by introducing an I44A mutation that disrupts a solvent exposed hydrophobic patch on ubiquitin that most ubiquitin binding proteins interact with [9, 12]. Notably, this mutation in the context of H2AKcl5ub(I44A) interferes with 53BP1 interaction with ubiquitinated H2A, yet does not interfere with the ability of i53 to enhance HDR, consistent with i53 enhancing HDR through interaction with the 53BP1 Tudor domain and not the UDR domain [9, 11]. Additionally, i53 was modified relative to G08 through the removal of the C-terminal di-glycine motif Introduction of i53, but not a 53BP1 binding deficient i53 variant DM (i53 P69L+L70V), into cells inhibited the formation ionizing radiation induced 53BP1 foci. Introduction of i53 via plasmid delivery, adeno-associated virus mediated gene delivery, or delivery of mRNA were all shown to improve the rates of HDR. Rates of HDR were improved with the introduction of i53 using both double-stranded DNA donors and using single-stranded DNA donors, which have been shown to use different HDR mechanisms [11, 13, 14]. 
     The present disclosure pertains to ubiquitin polypeptide variants (Ubvs) with increased affinity for 53BP1 and improved efficacy for enhancing HDR rates, and in particular, candidate amino acid changes in i53 that improve its affinity for 53BP1. Methods to identify such variants from a population of mutagenized ubiquitin polypeptides are provided, as well as the identification of additional beneficial mutations at specific amino acid positions. Improving the rate of HDR allows for increased rates of successful genome editing using the CRISPR/Cas9 system or other targeted nucleases in conjunction with supplying a repair template to direct precise genome editing events. 
     BRIEF SUMMARY OF THE INVENTION 
     In a first aspect, an isolated polypeptide comprising a ubiquitin polypeptide variant is provided. The isolated polypeptide comprises at least one member selected from one of the following groups: 
     SEQ ID NO:450, wherein X 1  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 2  is selected from Q, L, I, and M; X 6  is selected from K and R; X 7  is selected from T, M, I, C, L, and V; X 9  is selected from T, I, S, E and V; X 12  is selected from T, M, and Y; X 13  is selected from I, F, H and P; X 14  is selected from T, E, D, H, and N; X 16  is selected from E, M, T, N, Y, D, and H; X 17  is selected from V and C; X 18  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 19  is selected from P and K; X 20  is selected from S, D, N, C, A, and W; X 21  is selected from D and E; X 25  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 26  is selected from I, V, and L; X 28  is selected from A, E, Q, W, I, M, and D; X 29  is selected from K, M, L, R, Q, and H; X 3 1 is selected from Q, C, F, W, H, Y, L, R, and M; X 32  is selected from D, A, E, and R; X 33  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 34  is selected from E and T; X 38  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 39  is selected from D, W, E, G, S, L, and Q; X 40  is selected from Q, E, and D; X 41  is selected from Q, Y, I, C, and V; X 42  is selected from R, W, F, H, Y, N, C, and S; X 44  is selected from I, A and T; X 46  is selected from A, Q, and G; X 48  is selected from K, T, M, I, Q, V, R, L, and N; X 49  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 51  is selected from E and D; X 52  is selected from D and E; X 54  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 5s  is selected from T and R; X 57  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 5s  is selected from D and S; X 60  is selected from N, E, and Q; X 61  is selected from I and L; X 62  is selected from Q, L, T, V, C, A, M, I and S; X 63  is selected from K, I, M, F, and V; X 64  is selected from E, D, and S; X 65  is selected from S, P, E, K, H, R, A, D, N, and Q; X 66  is selected from T, K, R, and E; X 67  is selected from L, H, K, R, S, M, C, Y, and T; X 68  is selected from H, M, Q, and E; X 69  is selected from L, P, R, A, G, C, F, M, and S; X 70  is selected from V, L, M, F, and C; X 73  is selected from L and M; and X 74  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that SEQ ID NOS:1-3 are excluded; and 
     at least one member selected from the group of SEQ ID NOs:452-665. 
     In a second aspect, an isolated polypeptide comprising an isolated fusion polypeptide having an Ubv amino acid sequence with an N-terminal His 6 -tag is provided. The isolated fusion polypeptide comprises at least one member selected from the following: an isolated fusion polypeptide comprising SEQ ID NO:1100, wherein X 12  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 13  is selected from Q, L, I, and M; X 17  is selected from K and R; X 18  is selected from T, M, I, C, L, and V; X 20  is selected from T, I, S, E and V; X 23  is selected from T, M, and Y; X 24  is selected from I, F, H and P; X 25  is selected from T, E, D, H, and N; X 27  is selected from E, M, T, N, Y, D, and H; X 28  is selected from V and C; X 29  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 30  is selected from P and K; X 31  is selected from S, D, N, C, A, and W; X 32  is selected from D and E; X 36  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 37  is selected from I, V, and L; X 39  is selected from A, E, Q, W, I, M, and D; X 40  is selected from K, M, L, R, Q, and H; X 42  is selected from Q, C, F, W, H, Y, L, R, and M; X 43  is selected from D, A, E, and R; X 44  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 45  is selected from E and T; X 49  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 50  is selected from D, W, E, G, S, L, and Q; X 51  is selected from Q, E, and D; X 52  is selected from Q, Y, I, C, and V; X 53  is selected from R, W, F, H, Y, N, C, and S; X 5s  is selected from I, A and T; X 57  is selected from A, Q, and G; X 59  is selected from K, T, M, I, Q, V, R, L, and N; X 60  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 62  is selected from E and D; X 63  is selected from D and E; X 65  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 66  is selected from T and R; X 68  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 69  is selected from D and S; X 71  is selected from N, E, and Q; X 72  is selected from I and L; X 73  is selected from Q, L, T, V, C, A, M, I and S; X 74  is selected from K, I, M, F, and V; X 75  is selected from E, D, and S; X 76  is selected from S, P, E, K, H, R, A, D, N, and Q; X 77  is selected from T, K, R, and E; X 78  is selected from L, H, K, R, S, M, C, Y, and T; X 79  is selected from H, M, Q, and E; X 80  is selected from L, P, R, A, G, C, F, M, and S; X 81  is selected from V, L, M, F, and C; X 84  is selected from L and M; and X 85  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that SEQ ID NO: 3 is excluded; and an isolated fusion polypeptide comprising at least one member selected SEQ ID NOS:235-244 and 246-449. 
     In a third aspect, an isolated polypeptide that enhances HDR activity through interactions with 53BP1 in a manner to influence repair mechanisms at DSB sites is provided. The isolated polypeptide includes a Ubv having at least 40% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 40% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. The isolated polypeptide provides enhanced HDR activity through interactions with 53BP1 in a manner to influence repair mechanisms at DSB sites relative to SEQ ID NO:1 under identical conditions. 
     In a fourth aspect, an isolated polynucleotide is provided. The isolated polynucleotide encodes the isolated polypeptide of any of the first, second, or third aspects. 
     In a fifth aspect, an isolated polynucleotide encoding a ubiquitin polypeptide variant is provided. The isolated polynucleotide comprises at least one member selected from SEQ ID NOS:669-682, 885-890, and 892-1099, and the corresponding RNA counterparts thereof. 
     In a sixth aspect, a vector comprising an isolated polynucleotide encoding a ubiquitin polypeptide variant is provided. The isolated polynucleotide comprises at least one member selected from SEQ ID NOS:669-682, 885-890, and 892-1099, and the corresponding RNA counterparts thereof. 
     In a seventh aspect, a cell or cell line comprising the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In an eighth aspect, a method of suppressing 53BP1 recruitment to DNA double-strand break sites in a cell is provided. The method includes a step of administering to the cell the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In a ninth aspect, a method of increasing homology-directed repair in a cell is provided. The method includes a step of administering to the cell the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In a tenth aspect, a method of editing a gene in a cell using a CRISPR system is provided. The method includes a step of administering to the cell the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In an eleventh aspect, a method of gene targeting in a cell is provided. The method includes a step of administering to the cell the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In a twelfth aspect, a composition comprising the isolated polypeptide the isolated polypeptide of the first, second or third aspects is provided. 
     In an thirteenth aspect, a kit comprising the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In a fourteenth aspect, a method of performing a medically therapeutic procedure is provided. The includes the step of performing genome editing according to any of the tenth or eleventh aspects. 
     In a fifteenth aspect, a method of screening for amino acid changes in a first polypeptide that improve affinity of the first polypeptide for a second polypeptide is provided. The method includes a step of using the BACTH system with a reporter gene under control of cAMP regulated promoter to allow fluorescence activated cell sorting based on protein-protein interaction affinity between the first polypeptide and the second polypeptide to screen for improved affinity variants of the first polypeptide. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG.  1    depicts exemplary reporter gene expression being dependent on the Ubv expressed as part of the two-hybrid system. The graphs show gating and distribution of reporter signal versus forward scatter for cells grown under moderate selection pressure expressing the 53BP1-two-hybrid component fusion protein along with i53, i53 53BP1-binding-deficient mutant (DM), or i53+K33A fusion proteins (K33A was identified as beneficial from our screen). 
         FIG.  2    depicts exemplary studies showing enrichment of individual amino acid changes had high correlation between experiments. The graph shows the average enrichment of individual amino acid (a.a.) changes between two experiments with different levels of selection pressure. Testing of i53 in the context of the two-hybrid screen resulted in ˜17% and ˜3% GFP positive population in the low and high selection pressure experiments, respectively. Error bars indicate standard deviation between two replicates for each experiment. The data shown is only for the 1010 a.a. changes for which there was at least 30 reads in the input for both replicates for both experiments. 
         FIG.  3    depicts exemplary studies showing positive enrichment values from the high-throughput screen correlate well with an increased two-hybrid reporter positive population when amino acid changes are screened individually. The graph shows the percent of reporter positive cells containing the Ubv fusion protein plasmid with the indicated amino acid change compared to the average enrichment measured from the low selection pressure screen. Vertical error bars indicate standard deviation from three biological replicates. Horizontal error bars indicate standard deviation from two biological replicates. Asterisks indicate a significant increase in the percentage of reporter positive cells with the indicated amino acid change relative to i53 (p&lt;0.05, Dunnett&#39;s multiple comparisons test). The pooled screen enrichment indicated for i53 is for the unmodified plasmid relative to the pool of synonymous changes. 
         FIG.  4    depicts exemplary graphical data showing that Ubvs containing mutations identified by the two-hybrid screen in  E. coli  have improved in vitro affinity for the 53BP1 fragment. The graph plots the percent reporter positive cells expressing a fusion protein of the indicated Ubv plus a protein fragment used for the two hybrid system versus the affinity of purified i53 for a fragment of 53BP1 (Table 2) measured by BLI. The percentage of cells that are positive for reporter expression is an indication of the strength of the interaction in the two-hybrid screen. Ubvs consist of the i53 sequence plus the indicated amino acid substitutions or with no substitutions (i53). For the two-hybrid screen, Ubvs were tested individually and the data indicate the average of three replicates. The line is a simple linear regression of the data plotted in Prism with the R 2  value indicated. 
         FIG.  5 A  depicts an exemplary graph showing the association constant (1/dissociation constant) values measured in vitro using BLI of Ubvs proteins purified from  E. coli  (Table 3). The values are those calculated from the Kon and Kdis calculated from the 1:1 model fit of the protein association and dissociation (Table 4) 
         FIG.  5 B  depicts an exemplary graph showing the measured BLI response (Table 4) for i53, CM1, and CM7 interaction with the 53BP1 fragment (Table 3). The response curve was plotted using Prism using a one site-specific binding nonlinear fit model with the calculated dissociation constant (K d ) and R 2  indicated. 
         FIG.  5 C  depicts exemplary graphs showing BLI response vs time for the association and dissociation steps (non-red colored lines) for the data used for part B, with the calculated model fit indicated by the red lines. The top line for each graph is for the association using 20.5 μM of the 53BP1 fragment, with each line below indicating the response with a decreasing amount of 53BP1 down to 0.0102 μM (see Table 4). 
         FIG.  5 D  shows the sequences of human ubiquitin compared to i53, CM1, and CM7. The blue highlighting indicates the amino acid changes identified in the original i53 publication as part of G08. The green highlighting indicates the amino acid changes in the CM1 and CM7 ubiquitin variants. The red highlighting indicates that I44A mutation of i53 that is thought to disrupt interaction with ubiquitin binding proteins other than 53BP1. 
         FIG.  6    depicts an exemplary graph showing the rate of perfect HDR (introduction of a 6 nucleotide sequence at a Cas9 cut site in SERPINC1) measured by NGS in response to increasing amounts of Ubvs used during nucleofection in HEK293 cells. The dotted line indicates the level of HDR with no Ubv added. 
         FIG.  7    depicts a majority of tested high enrichment score amino acid changes from the two-hybrid screen resulted in improved affinity for 53BP1 when added to i53. The graph shows fold change in affinity measured by BLI of Ubvs that have a single mutation identified from the two-hybrid screen added to the i53 sequence. 
         FIG.  8    depicts nine mutations in CM1 relative to i53 contribute to the affinity of binding to 53BP1. The graph shows the fold change in affinity measured by BLI of Ubvs that lack the indicated mutation relative to CM1 (Table 6). 
         FIG.  9 A  depicts identification of ubiquitin variants with improved affinity over CM1. The graph shows the fold change in affinity for 53BP1 measured by BLI of ubiquitin variants that possess single amino acid substitutions added to CM1. 
         FIG.  9 B  shows the fold change in affinity of ubiquitin variants for 53BP1 measured by BLI that possess multiple mutations added simultaneously to the mutations in CM1. 
         FIG.  9 C  shows the fold change in affinity of ubiquitin variants that have groups of mutations identified or modified from those listed in  FIG.  9 B . added to CM1 simultaneously. 
         FIG.  9 D  shows the mutations present in the variants in  FIGS.  9 B and  9 C  relative to the sequence of i53 (SEQ ID NO: 2). 
         FIG.  10 A  shows higher affinity variants with additional stacked mutations better tolerate the introduction of 53BP1 binding deficient mutations. The graph shows the affinity (association constant K a ) of ubiquitin variants with and without the DM mutations (P69L, L70V). The sequences for the variants can be found in Table 6 (CM1-DM=CM107, CM138-DM=CM199, CM142-DM=CM203, CM143-DM=CM204, CM147-DM=CM208, CM149-DM=CM210, and CM158-DM=CM211) 
         FIG.  10 B  shows the rate of HDR (introduction of a 6 nucleotide sequence at a Cas9 cut site in SERPINC1) measured by EcoR1 cleavage of DNA PCR amplified from genomic DNA in response to increasing amounts of Ubvs used during nucleofection in HEK293 cells. The dashed line indicates the level of HDR with no Ubv added. 
         FIG.  11 A  shows screening of positions 69 and 70 mutations that allow for high affinity ubiquitin variants containing none of the published i53 mutations. The graphs show the fold change in affinity for amino acid changes at position 69 or 70 introduced into CM142 DM (CM203). 
         FIG.  11 B  shows the affinity for a fragment of 53BP1 of ubiquitin variants containing combinations of mutations at positions 69 and 70 with CM476 as the base construct. CM476 is a derivative of CM142 DM (CM203) with the remaining unchanged i53 mutation positions (2, 62, 64, and 66) mutated to the amino acid with the second best enrichment score from the two-hybrid screen. 
         FIG.  11 C  shows the fold change in affinity of variants containing mutations at position 62 relative to the base construct (CM429) containing a proline at position 62. 
         FIG.  11 D  shows a comparison of the affinity of i53, CM7, CM1, and CM455 measured by BLI. 
         FIG.  11 E  illustrates the sequence comparison of the proteins in  FIG.  11 D . 
         FIG.  11 F  shows the rate of perfect HDR (introduction of a 6 nucleotide sequence at a Cas9 cut site in SERPINC1) measured by NGS in response to increasing amounts of Ubvs used during nucleofection in HEK293 cells. The dashed line indicates the level of HDR with no Ubv added. The data shown is for two replicates with a line connecting the means. 
         FIG.  12 A  illustrates use of a ubiquitin variant with high affinity for 53BP1 provides an additional benefit to HDR over the use of a DNA-PK inhibitor alone. The graph shows the rate of HDR (introduction of 729 bp coding sequence for GFP at a Cas9 cut site in CLTA, Table 7) measured by Oxford Nanopore Technology (ONT) sequencing using Cas9 RNP delivered by nucleofection with 37.5 i53 or CM1 and/or IDT Enhancers (IDT-E or Alt_R HDR Enhancer) as an HDR enhancer in K562 cells. Ubiquitin variants were delivered alongside 2 μM Cas9 RNP at 37.5 μM final concentration. IDT-E was added to media post nucleofection for 24 hours at 1 μM final dose. Double stranded DNA donor with 200 bp homology arms was delivered at 1.5 μg per nucleofection. 
         FIG.  12 B  shows the rate of HDR (introduction of a 6 nucleotide sequence at a Cas9 cut site in MET) measured by EcoR1 cleavage of DNA PCR amplified from genomic DNA from HEK293 cells edited with Cas9 RNP targeting MET (Table 7) using Lonza nucleofection with either 12.5 μM CM1 co-delivered with 2 μM Cas9 RNP and/or treatment with 1 μM IDT-E for 24 hours with 1 μM Alt-R HDR donor oligo (Table 7). 
         FIG.  13    depicts screening of amino acid changes at position 2 of CM455 (SEQ ID NO:633) identified a more beneficial amino acid change. The graph shows the fold change in affinity for ubiquitin variants (CM489 (SEQ ID NO:658), CM455 (SEQ ID NO:633), CM478 (SEQ ID NO:647), CM479 (SEQ ID NO:648), CM480 (SEQ ID NO:649), CM481 (SEQ ID NO:650), CM483 (SEQ ID NO:652), CM485 (SEQ ID NO:654), CM486 (SEQ ID NO:655), CM487 (SEQ ID NO:656), CM488 (SEQ ID NO:657), CM490 (SEQ ID NO:659), CM491 (SEQ ID NO:660), CM492 (SEQ ID NO:661), CM493 (SEQ ID NO:662), CM494 (SEQ ID NO:663), CM495 (SEQ ID NO:664), and CM496 (SEQ ID NO:665)) containing a mutation at position 2 (relative to position 1 of WT ubiquitin (SEQ ID NO: 1)) of CM455 (SEQ ID NO:633). Fold change in affinity measured by BLI is shown relative CM489 (SEQ ID NO:658) which has a leucine at position 2. 
         FIG.  14    depicts a summary of amino acid sequences located in the wild-type human ubiquitin polypeptide (SEQ ID NO:1), i53 (SEQ ID NO:2), and the preferred ubiquitin polypeptide variant sequences (SEQ ID NO:450), wherein the preferred amino acid changes are listed below from top (highest) to bottom (lowest) average enrichment score from replicate experiments (see Examples). The dark grey background amino acids present in i53 that are not present in wildtype human ubiquitin. The non-underlined amino acid changes listed below the 3 reference sequences had a positive average enrichment score (average of two same day replicates) when added to i53 in at least one of two experiments. The single-underlined amino acid changes were identified as beneficial using BLI experiments in specific backgrounds (See Example 4 and Example 6). The double-underlined amino acid changes used in CM455 that were identified from the screen as having the highest enrichment score at that position (even if it was slightly negative). The light grey-shaded amino acid changes meet the same criteria as the non-underlined amino acids and were also described as potentially beneficial in the patent for i53 (SEQ ID NO:2) (WO2017132746A1. The black background shaded amino acid (i.e., position 67, K) is an amino acid change that meets the same criteria as the non-underlined amino acids but was also identified as potentially beneficial in the patent for i53 (SEQ ID NO:2) (see EP3411391 (B1) to Durocher et al.). 
         FIG.  15    demonstrates tag-free CM1 (CM1tf) is as active as His 6 -tagged CM1 in boosting rates of HDR. The graph shows the percent HDR measured by EcoR1 cleavage assay with varying amounts of CM1 (His 6 -tagged CM1; SEQ ID NO:241) or tag-free CM1 (CM1tf, SEQ ID NO:482). Cas9 RNP (2 μM) targeting HPRT1 (Table 7) was delivered with varying amounts of ubiquitin variant (50 μM to 1.56 μM in two fold increments) into cells by Lonza nucleofection along with 2 μM HDR donor (40 bp homology arms, 6 bp EcoR1 cut site insert). Data is shown for two biological replicates with lines connecting the means. The dashed line indicates the level of EcoR1 cleavage when no enhancer is used (n=3, standard deviation &lt;2%). 
         FIG.  16 A  depicts a graph showing the rate of HDR measured by EcoR1 cleavage assay in HEK293 cells that constitutively express HiFi Cas9 when plasmid (154 ng) encoding Cas9 sgRNA targeting HPRT1 plus 2 μM ssDNA donor (Table 7) was introduced into cells by Lonza Nucleofection. Plasmid (154 ng) for expression of His-tagged i53, His-tagged CM1, or a crRNA for LbCas12a (negative control) was co-delivered with the sgRNA expression plasmid and ssDNA donor as indicated. Error bars indicate the standard deviation from two replicates. 
         FIG.  16 B  depicts a graph shows the rate of HDR measured by EcoR1 cleavage assay in Jurkat cells which had CM1tf delivered as either mRNA or protein. CM1tf protein or mRNA encoding CM1tf was delivered with 2 μM Cas9 RNP targeting HPRT1 and 2 μM ssDNA donor (Table 7) into Jurkat cells by Lonza nucleofection. Error bars indicate the standard deviation from three replicates. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The current invention provides novel ubiquitin variants (Ubvs) with increased affinity for 53BP1 and improved efficacy for enhancing HDR rates. The identified Ubvs have increased affinity for 53BP1 and improved efficacy for enhancing HDR rates. Among the identified Ubvs include candidate amino acid changes in i53 that would improve its affinity for 53BP1 as well as Ubvs that do not include any of mutations present in the published i53 sequence. Methods to identify such variants from a population of mutagenized ubiquitin polypeptides are provided, as well as the identification of additional beneficial mutations at specific amino acid positions. Methods are provided that improve the rate of HDR and allow for increased rates of successful genome editing using the CRISPR/Cas9 system or other targeted nucleases in conjunction with supplying a repair template to direct precise genome editing events. 
     Screening methods to identify novel ubiquitin polypeptide variants 
     An initial filing identified ubiquitin variants (Ubvs) with increased affinity for 53BP1 and improved efficacy for enhancing HDR rates. In order to identify mutations that improve the affinity of i53 for 53BP1, a two-hybrid screen was conducted to identify variants with improved affinity. We engineered the screen such that interaction of two candidate proteins is tied to expression of a reporter gene that can be measured by fluorescence activated cell sorting (FACS). That disclosure described the results of a screen that interrogated the effect of all possible single amino acid substitutions individually at every position in i53 (a.a. 1-74) on the expression of a reporter gene in a two-hybrid assay in  E. coli . From that screening method, about 230 amino acid changes were identified as candidates for improving the affinity of i53 for 53BP1. Of the 24 amino acid changes tested individually, 16 of them resulted in a statistically significant increase in percent of cells that were positive for reporter expression relative to i53. See Example 1 for details. See U.S. Provisional Patent Application Ser. No. 63/248,300, filed Sep. 24, 2021, and entitled “UBIQUITIN VARIANTS WITH IMPROVED AFFINITY FOR 53BP1” (Attorney Docket No. IDT01-021-PRO), the contents of which is incorporated by reference in its entirety. 
     A subsequent filing described the testing of a subset of those mutations individually and in combination for their effects on the affinity of the two proteins in vitro and on the ability to enhance HDR. From this testing, several individual mutations that change amino acids at the surface of i53 that interacts with 53BP1 were found to significantly improve the affinity of i53 for 53BP1. When mutations were combined together, the highest affinity Ubv (CM1) had a 50 to 100 fold improvement in the affinity for a fragment of 53BP1 relative to the published i53 sequence. Two of the Ubvs that contain multiple mutations relative to i53 were tested for their ability to improve HDR in HEK293 cells. These tests revealed that the improved affinity ubiquitin variants require about a 10 fold lower dose for maximum effectiveness and that HDR rates were improved beyond what could be achieved with the i53 peptide. See U.S. Provisional Patent Application Ser. No. 63/278,155, filed Nov. 11, 2021, and entitled “UBIQUITIN VARIANTS WITH IMPROVED AFFINITY FOR 53BP1” (Attorney Docket No. IDT01-021-PRO2), the contents of which is incorporated by reference in its entirety. 
     A subsequent filing evaluated additional individual mutations in the context of i53 and CM1 and identified novel combinations of mutations that further improve affinity beyond that of CM1. Additionally, novel beneficial mutations beyond those identified in the screen at specific amino acid positions were identified. Combining the novel beneficial mutations with screen identified mutations resulted in the generation of Ubvs that do not include any of the mutations present in the published i53 sequence and have dramatically improved affinity for 53BP1 compared to i53. See U.S. Provisional Patent Application Ser. No. 63/321,384, filed Mar. 18, 2022 and entitled “UBIQUITIN VARIANTS WITH IMPROVED AFFINITY FOR 53BP1” (Attorney Docket No. IDT01-021-PRO3), the contents of which is incorporated by reference in its entirety. 
     Using a combination of amino acid changes from the two-hybrid screen and identified through specific position screens (see Example 4), a ubiquitin variant (CM455) was identified that does not contain any of the mutations present in i53 yet maintains affinity comparable to CM1. Additional individual mutations in the context of CM455 at position 2 were evaluated and identified a novel mutation that that results in a variant (CM487) with improved affinity beyond that of CM455. (See Example 6). 
     Isolated Ubiquitin Polypeptide Variants 
     Referring to  FIG.  14   , preferred isolated Ubv amino acid sequences include those summarized by SEQ ID NO:450: 
                            N-XXIFVXXLXG KXXXLXXXXX XTIEXXKXXI XXXXGIPXXX                        XXLXFXGXXL XXGXXLXXYX XXXXXXXXXX LRXX-C            
wherein X 1  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 2  is selected from Q, L, I, and M; X 6  is selected from K and R; X 7  is selected from T, M, I, C, L, and V; X 9  is selected from T, I, S, E and V; X 12  is selected from T, M, and Y; X 13  is selected from I, F, H and P; X 14  is selected from T, E, D, H, and N; X 16  is selected from E, M, T, N, Y, D, and H; X 17  is selected from V and C; X 18  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 19  is selected from P and K; X 20  is selected from S, D, N, C, A, and W; X 21  is selected from D and E; X 25  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 26  is selected from I, V, and L; X 28  is selected from A, E, Q, W, I, M, and D; X 29  is selected from K, M, L, R, Q, and H; X 31  is selected from Q, C, F, W, H, Y, L, R, and M; X 32  is selected from D, A, E, and R; X 33  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 34  is selected from E and T; X 38  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 39  is selected from D, W, E, G, S, L, and Q; X 40  is selected from Q, E, and D; X 41  is selected from Q, Y, I, C, and V; X 42  is selected from R, W, F, H, Y, N, C, and S; X 44  is selected from I, A and T; X 46  is selected from A, Q, and G; X 48  is selected from K, T, M, I, Q, V, R, L, and N; X 49  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 51  is selected from E and D; X 52  is selected from D and E; X 54  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 55  is selected from T and R; X 57  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 58  is selected from D and S; X 60  is selected from N, E, and Q; X 61  is selected from I and L; X 62  is selected from Q, L, T, V, C, A, M, I and S; X 63  is selected from K, I, M, F, and V; X 64  is selected from E, D, and S; X 65  is selected from S, P, E, K, H, R, A, D, N, and Q; X 66  is selected from T, K, R, and E; X 67  is selected from L, H, K, R, S, M, C, Y, and T; X 68  is selected from H, M, Q, and E; X 69  is selected from L, P, R, A, G, C, F, M, and S; X 70  is selected from V, L, M, F, and C; X 73  is selected from L and M; and X 74  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof. These polypeptides of SEQ ID NO:450 are highly preferred, provided that polypeptides encoding SEQ ID NOS:1-3 are excluded.
 
Fusion Polypeptides with Ubvs Polypeptides Fused to Affinity Tag Motifs
 
     Preferred Ubvs amino acid sequences include fusion polypeptides. Fusion polypeptides typically include extra amino acid information that is not native to the polypeptide to which the extra amino acid information is covalently attached. Such extra amino acid information may include tags that enable purification or identification of the fusion protein. Such extra amino acid information may also include peptides added to facilitate protein translation. Examples of such tags including adding an methionine or a methionine plus a short flexible linker (GGSG) (MGGSG; (SEQ ID NO:1113) to facilitate translation of protein variants where the X 1  is not M, such as in CM142 (SEQ ID NO: 557). Such extra amino acid information may include peptides that enable the fusion proteins to be transported into cells and/or transported to specific locations within cells such as peptides that act as nuclear localization signals. Examples of tags for these purposes include the following: AviTag, which is a peptide allowing biotinylation by the enzyme BirA so the protein can be isolated by streptavidin (GLNDIFEAQKIEWHE; SEQ ID NO:1114); Calmodulin-tag, which is a peptide bound by the protein calmodulin (KRRWKKNFIAVSAANRFKKISSSGAL; SEQ ID NO:1115); polyglutamate tag, which is a peptide binding efficiently to anion-exchange resin such as Mono-Q (EEEEEE; SEQ ID NO:1116); E-tag, which is a peptide recognized by an antibody (GAPVPYPDPLEPR; SEQ ID NO:1117); FLAG-tag, which is a peptide recognized by an antibody (DYKDDDDK; SEQ ID NO:1118); HA-tag, which is a peptide from hemagglutinin recognized by an antibody (YPYDVPDYA; SEQ ID NO:1119); His-tag, which is typically 5-10 histidines and can direct binding to a nickel or cobalt chelate (HHHHH; SEQ ID NO:1120); Myc-tag, which is a peptide derived from c-myc recognized by an antibody (EQKLISEEDL; SEQ ID NO:1121); NE-tag, which is a novel 18-amino-acid synthetic peptide (TKENPRSNQEESYDDNES; SEQ ID NO:1122) recognized by a monoclonal IgG1 antibody, which is useful in a wide spectrum of applications including Western blotting, ELISA, flow cytometry, immunocytochemistry, immunoprecipitation, and affinity purification of recombinant proteins; S-tag, which is a peptide derived from Ribonuclease A (KETAAAKFERQHMDS; SEQ ID NO:1123); SBP-tag, which is a peptide which binds to streptavidin; (MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP; SEQ ID NO:1124); Softag 1, which is intended for mammalian expression (SLAELLNAGLGGS; SEQ ID NO:1125); Softag 3, which is intended for prokaryotic expression (TQDPSRVG; SEQ ID NO:1126); Strep-tag, which is a peptide which binds to streptavidin or the modified streptavidin called streptactin (Strep-tag II: WSHPQFEK; SEQ ID NO:1127); TC tag, which is a tetracysteine tag that is recognized by FlAsH and ReAsH biarsenical compounds (CCPGCC; SEQ ID NO:1128) V5 tag, which is a peptide recognized by an antibody (GKPIPNPLLGLDST; SEQ ID NO:1129); VSV-tag, a peptide recognized by an antibody (YTDIEMNRLGK; SEQ ID NO:1130); Xpress tag (DLYDDDDK; SEQ ID NO:1131); Isopeptag, which is a peptide which binds covalently topilin-C protein (TDKDMTITFTNKKDAE; SEQ ID NO:1132); SpyTag, which is a peptide which binds covalently to SpyCatcher protein (AHIVMVDAYKPTK; SEQ ID NO:1133); and SnoopTag, a peptide which binds covalently to SnoopCatcher protein (KLGDIEFIKVNK; SEQ ID NO:1134). 
     An affinity tag can include flanking amino acids when the affinity tag is located at the N-terminus of the fusion polypeptide. Such flanking amino acids include an initiator methionine and flexible linker sequences. 
     A highly preferred affinity tag includes a His-tag (SEQ ID NO:1135). A highly preferred affinity tag includes an N-terminal His-tag (MHHHHHHGGSG; SEQ ID NO:1136). Highly preferred fusion polypeptides include Ubvs, such as SEQ ID NO: 3 fused to an N-terminal His-tag (e.g., SEQ ID NO:1136), as well as other preferred Ubvs amino acid sequences that include an N-terminal His-tag. A highly preferred translation tag includes N-terminal M (M) or M plus a short flexible linker (i.e., MGGSG: SEQ ID NO:1113). 
     A highly preferred fusion polypeptide of Ubvs comprises SEQ ID NO:1100: 
                            N-MHHHHHHGGSG XXIFVXXLXG KXXXLXXXXX XTIEXXKXXI                       XXXXGIPXXX XXLXFXGXXL XXGXXLXXYX XXXXXXXXXX                       LRXX-C            
wherein X 12  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 13  is selected from Q, L, I, and M; X 17  is selected from K and R; X 18  is selected from T, M, I, C, L, and V; X 20  is selected from T, I, S, E and V; X 23  is selected from T, M, and Y; X 24  is selected from I, F, H and P; X 25  is selected from T, E, D, H, and N; X 27  is selected from E, M, T, N, Y, D, and H; X 28  is selected from V and C; X 29  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 30  is selected from P and K; X 31  is selected from S, D, N, C, A, and W; X 32  is selected from D and E; X 36  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 37  is selected from I, V, and L; X 39  is selected from A, E, Q, W, I, M, and D; X 40  is selected from K, M, L, R, Q, and H; X 42  is selected from Q, C, F, W, H, Y, L, R, and M; X 43  is selected from D, A, E, and R; X 44  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 45  is selected from E and T; X 49  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 50  is selected from D, W, E, G, S, L, and Q; X 51  is selected from Q, E, and D; X 52  is selected from Q, Y, I, C, and V; X 53  is selected from R, W, F, H, Y, N, C, and S; X 55  is selected from I, A and T; X 57  is selected from A, Q, and G; X 59  is selected from K, T, M, I, Q, V, R, L, and N; X 60  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 62  is selected from E and D; X 63  is selected from D and E; X 65  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 66  is selected from T and R; X 68  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 69  is selected from D and S; X 71  is selected from N, E, and Q; X 72  is selected from I and L; X 73  is selected from Q, L, T, V, C, A, M, I and S; X 74  is selected from K, I, M, F, and V; X 75  is selected from E, D, and S; X 76  is selected from S, P, E, K, H, R, A, D, N, and Q; X 77  is selected from T, K, R, and E; X 78  is selected from L, H, K, R, S, M, C, Y, and T; X 79  is selected from H, M, Q, and E; X 80  is selected from L, P, R, A, G, C, F, M, and S; X 81  is selected from V, L, M, F, and C; X 84  is selected from L and M; and X 85  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that SEQ ID NO: 3 is excluded.
 
     Additional preferred fusion polypeptides of Ubvs include SEQ ID NOS:235-244 and 246-449. 
     Preferred Isolated Ubv Polypeptides Include Those Having Significant Amino Acid Sequence Identity to Reference Sequences. 
     An isolated polypeptide that enhances rates of HDR through interactions with 53BP1 in a manner to influence repair mechanisms at DSB sites is provided. The isolated polypeptide comprises a Ubv having at least 40% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 40% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Such an isolated polypeptide provides enhanced HDR activity through interactions with 53BP1 in a manner to influence repair mechanisms at DSB sites relative to SEQ ID NO:1 under identical conditions. 
     Preferred isolated polypeptides include those having amino acid sequence identity in the range of at least 50% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 50% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, preferred isolated polypeptides include those having amino acid sequence identity in the range of at least 60% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 60% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, preferred isolated polypeptides include those having amino acid sequence identity in the range of at least 70% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 70% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, preferred isolated polypeptides include those having amino acid sequence identity in the range of at least 80% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 80% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, preferred isolated polypeptides include those having amino acid sequence identity in the range of at least 90% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 90% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, preferred isolated polypeptides include those having amino acid sequence identity in the range of at least 95% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 95% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. 
     A preferred polypeptide sequence in the aforementioned ranges with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded, further provide a functional benefit of enhanced HDR rates when compared to HDR rates achieved when introducing human ubiquitin SEQ ID NO:1 into cells under identical conditions. 
     Evaluation of Isolated Polypeptides Having a Functional Benefit of Enhanced HDR Rates 
     A preferred isolated polynucleotide encoding such isolated polypeptides within the stated ranges of % amino acid sequence identity to the aforementioned reference polypeptide sequence(s) in the aforementioned ranges, further provide a functional benefit of enhanced HDR rates when compared to HDR achieved when introducing human ubiquitin SEQ ID NO:1 into cells under identical conditions. Such enhanced HDR rates can be readily assessed by one of skill in the art based upon the teachings disclosed herein, including tests for at least one of the following functional properties: (1) a higher K a  (lower Kd) for binding a fragment of 53BP1 (amino acids 1484-1603) (See, for example, SEQ ID NO: 245) than is measured for Human ubiquitin (SEQ ID NO:1) under identical conditions as measured in vitro using BLI, even more preferably a higher measured K a  (lower K d ) for binding a fragment of 53BP1 (amino acids 1484-1603) (See SEQ ID NO: 245) than is measured for i53 (SEQ ID NO:2) under identical conditions as measured in vitro using BLI; (2) Delivery of the polypeptide in the form of mRNA, plasmid, or protein, results in improved HDR rates for introduction an EcoR1 cut site insert at the HPRT1 or SERPINC1 cut sites as specified by the sgRNA and ssDNA donor sequences in Table 7 as compared to delivery of human ubiquitin (SEQ ID NO: 1) under the same conditions. See Examples 3, 4, 7, and 8 for details. 
     Isolated Nucleic Acids 
     Isolated nucleic acids encoding preferred Ubvs amino acid sequences are provided. One preferred isolated nucleic acid encodes SEQ ID NO:450: 
     
       
         
           
               
               
            
               
                   
                 N-XXIFVXXLXG KXXXLXXXXX XTIEXXKXXI XXXXGIPXXX  
               
               
                   
                   
               
               
                   
                 XXLXFXGXXL XXGXXLXXYX XXXXXXXXXX LRXX-C 
               
            
           
         
       
     
     wherein X 1  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 2  is selected from Q, L, I, and M; X 6  is selected from K and R; X 7  is selected from T, M, I, C, L, and V; X 9  is selected from T, I, S, E and V; X 12  is selected from T, M, and Y; X 13  is selected from I, F, H and P; X 14  is selected from T, E, D, H, and N; X 16  is selected from E, M, T, N, Y, D, and H; X 17  is selected from V and C; X 18  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 19  is selected from P and K; X 20  is selected from S, D, N, C, A, and W; X 21  is selected from D and E; X 25  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 26  is selected from I, V, and L; X 28  is selected from A, E, Q, W, I, M, and D; X 29  is selected from K, M, L, R, Q, and H; X 31  is selected from Q, C, F, W, H, Y, L, R, and M; X 32  is selected from D, A, E, and R; X 33  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 34  is selected from E and T; X 38  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 39  is selected from D, W, E, G, S, L, and Q; X 40  is selected from Q, E, and D; X 41  is selected from Q, Y, I, C, and V; X 42  is selected from R, W, F, H, Y, N, C, and S; X 44  is selected from I, A and T; X 46  is selected from A, Q, and G; X 48  is selected from K, T, M, I, Q, V, R, L, and N; X 49  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 51  is selected from E and D; X 52  is selected from D and E; X 54  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 55  is selected from T and R; X 57  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 58  is selected from D and S; X 60  is selected from N, E, and Q; X 61  is selected from I and L; X 62  is selected from Q, L, T, V, C, A, M, I and S; X 63  is selected from K, I, M, F, and V; X 64  is selected from E, D, and S; X 65  is selected from S, P, E, K, H, R, A, D, N, and Q; X 66  is selected from T, K, R, and E; X 67  is selected from L, H, K, R, S, M, C, Y, and T; X 68  is selected from H, M, Q, and E; X 69  is selected from L, P, R, A, G, C, F, M, and S; X 70  is selected from V, L, M, F, and C; X 73  is selected from L and M; and X 74  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that polypeptides encoding SEQ ID NOS:1-3 are excluded (i.e., SEQ ID NOS: 666, 667 and 883). 
     Another preferred isolated nucleic acid encodes SEQ ID NO:1100: 
     
       
         
           
               
               
            
               
                   
                 N-MHHHHHHGGSG XXIFVXXLXG KXXXLXXXXX XTIEXXKXXI 
               
               
                   
                   
               
               
                   
                 XXXXGIPXXX XXLXFXGXXL XXGXXLXXYX XXXXXXXXXX 
               
               
                   
                   
               
               
                   
                 LRXX-C 
               
            
           
         
       
     
     wherein X 12  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 13  is selected from Q, L, I, and M; X 17  is selected from K and R; X 18  is selected from T, M, I, C, L, and V; X 20  is selected from T, I, S, E and V; X 23  is selected from T, M, and Y; X 24  is selected from I, F, H and P; X 25  is selected from T, E, D, H, and N; X 27  is selected from E, M, T, N, Y, D, and H; X 28  is selected from V and C; X 29  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 30  is selected from P and K; X 31  is selected from S, D, N, C, A, and W; X 32  is selected from D and E; X 36  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 37  is selected from I, V, and L; X 39  is selected from A, E, Q, W, I, M, and D; X 40  is selected from K, M, L, R, Q, and H; X 42  is selected from Q, C, F, W, H, Y, L, R, and M; X 43  is selected from D, A, E, and R; X 44  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 45  is selected from E and T; X 49  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 50  is selected from D, W, E, G, S, L, and Q; X 51  is selected from Q, E, and D; X 52  is selected from Q, Y, I, C, and V; X 53  is selected from R, W, F, H, Y, N, C, and S; X 55  is selected from I, A and T; X 57  is selected from A, Q, and G; X 59  is selected from K, T, M, I, Q, V, R, L, and N; X 60  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 62  is selected from E and D; X 63  is selected from D and E; X 65  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 66  is selected from T and R; X 68  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 69  is selected from D and S; X 71  is selected from N, E, and Q; X 72  is selected from I and L; X 73  is selected from Q, L, T, V, C, A, M, I and S; X 74  is selected from K, I, M, F, and V; X 75  is selected from E, D, and S; X 76  is selected from S, P, E, K, H, R, A, D, N, and Q; X 77  is selected from T, K, R, and E; X 78  is selected from L, H, K, R, S, M, C, Y, and T; X 79  is selected from H, M, Q, and E; X 80  is selected from L, P, R, A, G, C, F, M, and S; X 81  is selected from V, L, M, F, and C; X 84  is selected from L and M; and X 85  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that SEQ ID NO: 3 is excluded. 
     Preferred isolated polynucleotides (e.g., DNA and their corresponding RNA counterparts) include those that encode Ubvs having an amino acid sequence identity in the range of at least 70% to 100% identity of SEQ ID NOS: 450 and 1100, respectively. Even more preferably, isolated polynucleotides include those that encode Ubvs having an amino acid sequence identity in the range of at least 80% to 100% identity of SEQ ID NOS: 450 and 1100, respectively. Even more preferably, preferred isolated polynucleotides include those that encode Ubvs having an amino acid sequence identity in the range of at least 90% to 100% identity of SEQ ID NOS: 450 and 1100, respectively. Even more preferably, preferred isolated polynucleotides include those that encode Ubvs having an amino acid sequence identity in the range of at least 95% to 100% identity of SEQ ID NOS: 450 and 1100, respectively. 
     Preferred Isolated Ubv Polynucleotides Include Those Having Significant Amino Acid Sequence Identity to Reference Sequences. 
     An isolated polynucleotide that encodes an isolated polypeptide with enhanced HDR activity through interactions with 53BP1 in a manner to influence repair mechanisms at DSB sites is provided. The encoded isolated polypeptide comprises a Ubv having at least 40% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 40% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Such an isolated polypeptide identity provides enhanced HDR activity through interactions with 53BP1 in a manner to influence repair mechanisms at DSB sites relative to SEQ ID NO:1 under identical conditions. 
     Preferred isolated polynucleotides encoding such isolated polypeptides include polypeptides those having amino acid sequence identity in the range of at least 50% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 50% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, isolated polynucleotides encoding such isolated polypeptides include those having amino acid sequence identity in the range of at least 60% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 60% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, isolated polynucleotides encoding such isolated polypeptides include those having amino acid sequence identity in the range of at least 70% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 70% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, isolated polynucleotides encoding such isolated polypeptides include those having amino acid sequence identity in the range of at least 80% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 80% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, preferred isolated polynucleotides encoding such isolated polypeptides include those having amino acid sequence identity in the range of at least 90% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 90% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. Even more preferably, preferred isolated polynucleotides encoding such isolated polypeptides include those having amino acid sequence identity in the range of at least 95% to 100% identity with amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having amino acid sequence identity in the range of at least 95% to 100% identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. 
     A preferred isolated polynucleotide encoding such isolated polypeptides within the stated ranges of % amino acid sequence identity to the aforementioned reference polypeptide sequence(s) in the aforementioned ranges, further provide a functional benefit of enhanced HDR rates when compared to HDR rates of an isolated polynucleotide encoding SEQ ID NO:1 under identical conditions. Such enhanced HDR rates can be readily assessed by one of skill in the art based upon the teachings disclosed herein, including evaluations as described previously herein. 
     Applications 
     It will be generally understood that the disclosed amino acid substitutions within the ubiquitin polypeptide variants that result in improved affinity for 53BP1 can be generated in the context of the wild-type ubiquitin polypeptide (SEQ ID NO:1) or the i53 ubiquitin polypeptide (SEQ ID NO:2), including tag-free polypeptides and fusion polypeptides having an affinity tag included as part of the ubiquitin polypeptide variants. For example, one skilled in the art will appreciate that untagged versions or differently tagged versions fall within the scope of the disclosed ubiquitin polypeptide variants, including those ubiquitin polypeptide variants having a polyhistidine motif (e.g., a His 6  tag). Accordingly, alternative versions of ubiquitin polypeptide variants may be constructed and function either with or without an affinity tag, such as a polyhistidine tag. 
     In a first aspect, an isolated polypeptide comprising a ubiquitin polypeptide variant is provided. The isolated polypeptide comprises at least one member selected from one of the following groups: 
     SEQ ID NO:450, wherein X 1  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 2  is selected from Q, L, I, and M; X 6  is selected from K and R; X 7  is selected from T, M, I, C, L, and V; X 9  is selected from T, I, S, E and V; X 12  is selected from T, M, and Y; X 13  is selected from I, F, H and P; X 14  is selected from T, E, D, H, and N; X 16  is selected from E, M, T, N, Y, D, and H; X 17  is selected from V and C; X 18  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 19  is selected from P and K; X 20  is selected from S, D, N, C, A, and W; X 21  is selected from D and E; X 25  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 26  is selected from I, V, and L; X 28  is selected from A, E, Q, W, I, M, and D; X 29  is selected from K, M, L, R, Q, and H; X 3 1 is selected from Q, C, F, W, H, Y, L, R, and M; X 32  is selected from D, A, E, and R; X 33  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 34  is selected from E and T; X 38  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 39  is selected from D, W, E, G, S, L, and Q; X 40  is selected from Q, E, and D; X 41  is selected from Q, Y, I, C, and V; X 42  is selected from R, W, F, H, Y, N, C, and S; X 44  is selected from I, A and T; X 46  is selected from A, Q, and G; X 48  is selected from K, T, M, I, Q, V, R, L, and N; X 49  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 51  is selected from E and D; X 52  is selected from D and E; X 54  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 55  is selected from T and R; X 57  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 58  is selected from D and S; X 60  is selected from N, E, and Q; X 61  is selected from I and L; X 62  is selected from Q, L, T, V, C, A, M, I and S; X 63  is selected from K, I, M, F, and V; X 64  is selected from E, D, and S; X 65  is selected from S, P, E, K, H, R, A, D, N, and Q; X 66  is selected from T, K, R, and E; X 67  is selected from L, H, K, R, S, M, C, Y, and T; X 68  is selected from H, M, Q, and E; X 69  is selected from L, P, R, A, G, C, F, M, and S; X 70  is selected from V, L, M, F, and C; X 73  is selected from L and M; and X 74  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that SEQ ID NOS:1-3 are excluded; and 
     at least one member selected from the group of SEQ ID NOs:452-665. 
     In a first respect, the isolated polypeptide comprises a ubiquitin polypeptide variant selected from SEQ ID NO:450, wherein X 1  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 2  is selected from Q, L, I, and M; X 6  is selected from K and R; X 7  is selected from T, M, I, C, L, and V; X 9  is selected from T, I, S, E and V; X 12  is selected from T, M, and Y; X 13  is selected from I, F, H and P; X 14  is selected from T, E, D, H, and N; X 16  is selected from E, M, T, N, Y, D, and H; X 17  is selected from V and C; X 18  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 19  is selected from P and K; X 20  is selected from S, D, N, C, A, and W; X 21  is selected from D and E; X 25  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 26  is selected from I, V, and L; X 28  is selected from A, E, Q, W, I, M, and D; X 29  is selected from K, M, L, R, Q, and H; X 31  is selected from Q, C, F, W, H, Y, L, R, and M; X 32  is selected from D, A, E, and R; X 33  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 34  is selected from E and T; X 38  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 39  is selected from D, W, E, G, S, L, and Q; X 40  is selected from Q, E, and D; X 41  is selected from Q, Y, I, C, and V; X 42  is selected from R, W, F, H, Y, N, C, and S; X 44  is selected from I, A and T; X 46  is selected from A, Q, and G; X 48  is selected from K, T, M, I, Q, V, R, L, and N; X 49  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 51  is selected from E and D; X 52  is selected from D and E; X 54  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 55  is selected from T and R; X 57  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 58  is selected from D and S; X 60  is selected from N, E, and Q; X 61  is selected from I and L; X 62  is selected from Q, L, T, V, C, A, M, I and S; X 63  is selected from K, I, M, F, and V; X 64  is selected from E, D, and S; X 65  is selected from S, P, E, K, H, R, A, D, N, and Q; X 66  is selected from T, K, R, and E; X 67  is selected from L, H, K, R, S, M, C, Y, and T; X 68  is selected from H, M, Q, and E; X 69  is selected from L, P, R, A, G, C, F, M, and S; X 70  is selected from V, L, M, F, and C; X 73  is selected from L and M; and X 74  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that SEQ ID NOS:1-3 are excluded. In a second respect, the isolated polypeptide shares amino acid sequence identity in the range of at least 40% to 100% identity of SEQ ID NO:1. In a third respect, the isolated polypeptide shares amino acid sequence identity in the range of at least 50% to 100% identity of SEQ ID NO:1. In a fourth respect, the isolated polypeptide shares amino acid sequence identity in the range of at least 60% to 100% identity of SEQ ID NO:1. In a fifth respect, the isolated polypeptide shares amino acid sequence identity in the range of at least 70% to 100% identity of SEQ ID NO:1. In a sixth respect, the isolated polypeptide shares amino acid sequence identity in the range of at least 80% to 100% identity of SEQ ID NO:1. In a seventh respect, the isolated polypeptide shares amino acid sequence identity in the range of at least 90% to 100% identity of SEQ ID NO:1. In an eighth respect, the isolated polypeptide shares amino acid sequence identity in the range of at least 95% to 100% identity of SEQ ID NO:1. 
     In a second aspect, an isolated polypeptide comprising an isolated fusion polypeptide having an Ubv amino acid sequence with an N-terminal His 6 -tag is provided. The isolated fusion polypeptide comprises at least one member selected from the following: an isolated fusion polypeptide comprising SEQ ID NO: 1100, wherein X 12  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 13  is selected from Q, L, I, and M; X 17  is selected from K and R; X 18  is selected from T, M, I, C, L, and V; X 20  is selected from T, I, S, E and V; X 23  is selected from T, M, and Y; X 24  is selected from I, F, H and P; X 25  is selected from T, E, D, H, and N; X 27  is selected from E, M, T, N, Y, D, and H; X 28  is selected from V and C; X 29  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 30  is selected from P and K; X 31  is selected from S, D, N, C, A, and W; X 32  is selected from D and E; X 36  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 37  is selected from I, V, and L; X 39  is selected from A, E, Q, W, I, M, and D; X 40  is selected from K, M, L, R, Q, and H; X 42  is selected from Q, C, F, W, H, Y, L, R, and M; X 43  is selected from D, A, E, and R; X 44  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 45  is selected from E and T; X 49  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 50  is selected from D, W, E, G, S, L, and Q; X 51  is selected from Q, E, and D; X 52  is selected from Q, Y, I, C, and V; X 53  is selected from R, W, F, H, Y, N, C, and S; X 55  is selected from I, A and T; X 57  is selected from A, Q, and G; X 59  is selected from K, T, M, I, Q, V, R, L, and N; X 60  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 62  is selected from E and D; X 63  is selected from D and E; X 65  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 66  is selected from T and R; X 68  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 69  is selected from D and S; X 71  is selected from N, E, and Q; X 72  is selected from I and L; X 73  is selected from Q, L, T, V, C, A, M, I and S; X 74  is selected from K, I, M, F, and V; X 75  is selected from E, D, and S; X 76  is selected from S, P, E, K, H, R, A, D, N, and Q; X 77  is selected from T, K, R, and E; X 78  is selected from L, H, K, R, S, M, C, Y, and T; X 79  is selected from H, M, Q, and E; X 80  is selected from L, P, R, A, G, C, F, M, and S; X 81  is selected from V, L, M, F, and C; X 84  is selected from L and M; and X 85  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that SEQ ID NO: 3 is excluded; and an isolated fusion polypeptide comprising at least one member selected SEQ ID NOS:235-244 and 246-449. 
     In a first respect, an isolated polypeptide comprising an isolated fusion polypeptide having an Ubv amino acid sequence with an N-terminal His 6 -tag is provided. The isolated fusion polypeptide comprises at least one member selected from the following: an isolated fusion polypeptide comprising SEQ ID NO: 1100, wherein X 12  is selected from M, H, Y, W, Q, T, F, S, R, I, and N; X 13  is selected from Q, L, I, and M; X 17  is selected from K and R; X 18  is selected from T, M, I, C, L, and V; X 20  is selected from T, I, S, E and V; X 23  is selected from T, M, and Y; X 24  is selected from I, F, H and P; X 25  is selected from T, E, D, H, and N; X 27  is selected from E, M, T, N, Y, D, and H; X 28  is selected from V and C; X 29  is selected from E, M, Y, L, H, F, W, S, Q, T, C, N, R, and D; X 30  is selected from P and K; X 31  is selected from S, D, N, C, A, and W; X 32  is selected from D and E; X 36  is selected from N, V, I, E, G, M, Q, D, A, L, R, S, K, T, C, and F; X 37  is selected from I, V, and L; X 39  is selected from A, E, Q, W, I, M, and D; X 40  is selected from K, M, L, R, Q, and H; X 42  is selected from Q, C, F, W, H, Y, L, R, and M; X 43  is selected from D, A, E, and R; X 44  is selected from K, H, A, Q, S, V, L, E, M, T, I, F, C, Y, R, N, and W; X 45  is selected from E and T; X 49  is selected from P, L, C, F, I, V, Y, T, M, H, S, Q, A, W, N, and K; X 50  is selected from D, W, E, G, S, L, and Q; X 51  is selected from Q, E, and D; X 52  is selected from Q, Y, I, C, and V; X 53  is selected from R, W, F, H, Y, N, C, and S; X 55  is selected from I, A and T; X 57  is selected from A, Q, and G; X 59  is selected from K, T, M, I, Q, V, R, L, and N; X 60  is selected from Q, S, L, M, P, E V, A, D, I, C, G, and N; X 62  is selected from E and D; X 63  is selected from D and E; X 65  is selected from R, Y, M, T, H, F, N, Q, K, and C; X 66  is selected from T and R; X 68  is selected from S, G, D, N, H, E, A, Q, M, R, and K; X 69  is selected from D and S; X 71  is selected from N, E, and Q; X 72  is selected from I and L; X 73  is selected from Q, L, T, V, C, A, M, I and S; X 74  is selected from K, I, M, F, and V; X 75  is selected from E, D, and S; X 76  is selected from S, P, E, K, H, R, A, D, N, and Q; X 77  is selected from T, K, R, and E; X 78  is selected from L, H, K, R, S, M, C, Y, and T; X 79  is selected from H, M, Q, and E; X 80  is selected from L, P, R, A, G, C, F, M, and S; X 81  is selected from V, L, M, F, and C; X 84  is selected from L and M; and X 85  is selected from R, Q, V, L, M, C, I, T, E, and K, and combinations thereof, provided that SEQ ID NO: 3 is excluded. In a second respect, the isolated polypeptide of SEQ ID 1100 encompassing amino acid positions 12-85 shares amino acid sequence identity in the range of at least 40% to 100% identity of SEQ ID NO:1. In a third respect, the isolated polypeptide of SEQ ID 1100 encompassing amino acid positions 12-85 shares amino acid sequence identity in the range of at least 50% to 100% identity of SEQ ID NO:1. In a fourth respect, the isolated polypeptide of SEQ ID 1100 encompassing amino acid positions 12-85 shares amino acid sequence identity in the range of at least 60% to 100% identity of SEQ ID NO:1. In a fifth respect, the isolated polypeptide of SEQ ID 1100 encompassing amino acid positions 12-85 shares amino acid sequence identity in the range of at least 70% to 100% identity of SEQ ID NO:1. In a sixth respect, the isolated polypeptide of SEQ ID 1100 encompassing amino acid positions 12-85 shares amino acid sequence identity in the range of at least 80% to 100% identity of SEQ ID NO:1. In a seventh respect, the isolated polypeptide of SEQ ID 1100 encompassing amino acid positions 12-85 shares amino acid sequence identity in the range of at least 90% to 100% identity of SEQ ID NO:1. In an eighth respect, the isolated polypeptide of SEQ ID 1100 encompassing amino acid positions 12-85 shares amino acid sequence identity in the range of at least 95% to 100% identity of SEQ ID NO:1. 
     In a third aspect, an isolated polypeptide that enhances rates of HDR through interactions with 53BP1 in a manner to influence repair mechanisms at DSB sites is provided. The isolated polypeptide includes a Ubv having at least 40% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 40% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. The isolated polypeptide provides enhanced HDR activity through interactions with 53BP1 in a manner to influence repair mechanisms at DSB sites relative to SEQ ID NO:1 under identical conditions. 
     In a first respect, the isolated polypeptide includes a Ubv having at least 50% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 50% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. In a second respect, the isolated polypeptide includes a Ubv having at least 60% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 60% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. In a third respect, the isolated polypeptide includes a Ubv having at least 70% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 70% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. In a fourth respect, the isolated polypeptide includes a Ubv having at least 80% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 80% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. In a fifth respect, the isolated polypeptide includes a Ubv having at least 90% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 90% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. In a sixth respect, the isolated polypeptide includes a Ubv having at least 95% amino acid sequence identity to amino acid positions 1-74 of SEQ ID NOS:1, 2, 482, 633, or 450, provided that SEQ ID NOS:1 and 2 are excluded, and those having at least 95% amino acid sequence identity with amino acid positions 12-85 of SEQ ID NOS: 3, 241, 417, or 1100, provided that SEQ ID NO:3 is excluded. 
     In a fourth aspect, an isolated polynucleotide is provided. The isolated polynucleotide encodes the isolated polypeptide of any of the first, second, or third aspects. 
     In a fifth aspect, an isolated polynucleotide encoding a ubiquitin polypeptide variant is provided. The isolated polynucleotide comprises at least one member selected from SEQ ID NOS:669-682, 885-890, and 892-1099, and the corresponding RNA counterparts thereof. 
     In a sixth aspect, a vector comprising an isolated polynucleotide encoding a ubiquitin polypeptide variant is provided. The isolated polynucleotide comprises at least one member selected from SEQ ID NOS:669-682, 885-890, and 892-1099, and the corresponding RNA counterparts thereof. 
     In a seventh aspect, a cell or cell line comprising the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In an eighth aspect, a method of suppressing 53BP1 recruitment to DNA double-strand break sites in a cell is provided. The method includes a step of administering to the cell the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In a ninth aspect, a method of increasing homologous recombination in a cell is provided. The method includes a step of administering to the cell the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In a tenth aspect, a method of editing a gene in a cell using a CRISPR system is provided. The method includes a step of administering to the cell the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In an eleventh aspect, a method of gene targeting in a cell is provided. The method includes a step of administering to the cell the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. 
     In a twelfth aspect, a composition comprising the isolated polypeptide the isolated polypeptide of the first, second or third aspects is provided. 
     In an thirteenth aspect, a kit comprising the isolated polypeptide of the first, second, or third aspects, the isolated polynucleotide of the fourth or fifth aspects, or the vector of the sixth aspect. In a first respect, the kit additionally includes one or more components of a gene editing system. In this regard, the gene editing system is a CRISPR system. 
     In a fourteenth aspect, a method of performing a medically therapeutic procedure is provided. The includes the step of performing genome editing according to any of the tenth or eleventh aspects. 
     In a fifteenth aspect, a method of screening for amino acid changes in a first polypeptide that improve affinity of the first polypeptide for a second polypeptide is provided. The method includes a step of using the BACTH system with a reporter gene under control of cAMP regulated promoter to allow fluorescence activated cell sorting based on protein-protein interaction affinity between the first polypeptide and the second polypeptide to screen for improved affinity variants of the first polypeptide. 
     The polypeptides and polynucleotides disclosed herein may be used in a broad spectrum of applications. The polypeptides and polynucleotides disclosed herein may be used for the detection and quantitative determination as well as for the separation and isolation of 53BP1. The polypeptides and polynucleotides disclosed herein may be used in genomic engineering, epigenomic engineering, genome targeting, and genome editing. The polypeptides and polynucleotides disclosed herein may be used to modify repair pathways, activate or stimulate HDR or homology-based genome editing, inhibit 53BP1 recruitment to DSB sites or damaged chromatin in a cell or modulate DNA end resection. In an aspect, the polypeptides and polynucleotides disclosed herein are used in combination with a gene editing system. The disclosure also provides the use of the polypeptides and polynucleotides disclosed herein as medicaments. 
     EXAMPLES 
     Example 1. A Two-Hybrid Screen Identified a Variety of Mutations that May Increase Ubiquitin Variant Affinity for 53BP1 
     In order to identify mutations that improve the affinity of i53 for 53BP1, the bacterial adenylate cyclase two-hybrid system (BACTH system) was used to screen for interaction between the two proteins. This method makes use of a  B. pertussis  calmodulin-dependent adenylate cyclase toxin. The catalytic domain of the toxin can be separated into two fragments (T18 and T25) that are able to associate in the presence of calmodulin but have minimal activity in its absence [21, 22]. If bait and prey proteins fused to T18 and T25 interact, then the catalytic activity is restored and cAMP is produced. In  E. coli , cAMP binds to catabolite activator protein (CAP) that acts as a transcriptional activator for several genes. By expressing these fusion proteins in an  E. coli  strain that lacks endogenous adenylate cyclase and naturally lacks calmodulin, cAMP regulated protein expression can be used as a readout of bait-prey interaction [23]. We engineered the screen so that eGFP will be expressed under the control of a cAMP-regulated promoter. The coding sequence for a fragment of 53BP1 (a.a. 1221-1718) containing the i53 interacting regions and i53 were cloned into T18 and T25 adenylate cyclase expression plasmids such that fusion proteins of each would be expressed. If a Ubv interacts with 53BP1, the T18 and T25 fragments will be brought together, adenylate cyclase activity will be restored, cAMP will be produced, and some portion of the bacterial population will be GFP positive. 
     A plasmid library was made consisting of Ubv-adenylate cyclase fragment fusion protein plasmids that had on average a single codon within the i53 coding region exchanged for a random NNK codon. Plasmids were transformed into DHM1 cells that lack endogenous adenylate cyclase and contain the plasmid for expression of the 53BP1 fragment fused to one of the adenylate cyclase fragments. Expression of eGFP was used as a readout of bait-prey interaction using fluorescence activated cell sorting (FACS) to sort for GFP positive bacteria. Plasmid DNA was isolated from both the sorted GFP positive bacteria (Positive) and from the original pre-sort population (Input) and was sequenced using NGS. Counts were merged for mutations that result in the same amino acid change using Enrich2 [25]. Enrichment was calculated as enrichment=log 2((read count for an amino acid change in the positive population/read count for an amino acid change in the input)/(synonymous change read count in the positive population/synonymous change read count in the input)). A positive enrichment value indicates that mutations resulting in a particular amino acid substitution result in a higher percent of GFP positive bacteria than synonymous mutations and therefore indicates that the amino acid change may improve i53 affinity for 53BP1. For each experiment, DHM1 cells were transformed with the Ubv fusion protein plasmid library in two separate replicates using a gene pulser (Bio-Rad). The i53-adenylate cyclase fragment fusion protein (published i53 peptide, SEQ ID NO:2) plasmid was also introduced separately as a control to estimate selection pressure. Cells were then grown and sorted using FACS and GFP positive cells were collected. Two separate experiments were conducted on separate days using different levels of selection pressure resulting in a different percent GFP positive for the i53 population (i.e. for cells that express published i53 peptide (SEQ ID NO:2) fused to one of the adenylate cyclase fragments). Experiment one had an i53 percent positive of approximately 30 and experiment two had an i53 percent GFP positive of approximately 1700. 
     There was a high degree of correlation between the two experiments and between replicates ( FIG.  2   ). From these screens, about 230 amino acid changes were identified for which the average enrichment was positive for at least one of the experiments (Table 1). These amino acid changes resulted in increased reporter gene (GFP) expression in our two-hybrid system and potentially improve the affinity of i53 for 53BP 1. To validate that the amino acid changes identified from the pooled screen are reproducible on an individual basis, 24 amino acid changes identified from this screen were introduced individually into the i53 fusion protein plasmid and tested by flow cytometry for their effect on the percent positive population relative to i53 ( FIG.  3   ). There was a strong correlation between the enrichment measured from the pooled screen and the percent reporter positive cells when mutations were screened individually. Of the 24 mutations tested individually, 16/24 mutations had a statistically significant increase in percent positive relative to i53 wild type (Table 2). 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Amino acid changes with positive average enrichment in at least one experiment 1   
               
            
           
           
               
               
               
               
            
               
                   
                 Amino 
                 Experiment 1 - high selection pressure 
                 Experiment 2-low selection pressure 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 SEQ ID 
                 acid 
                 Rep 1 
                 Rep 2 
                 Average 
                 Rep 1 
                 Rep 2 
                 Average 
               
               
                 NO: 
                 change 
                 Enrichment 
                 Enrichment 
                 Enrichment 
                 Enrichment 
                 Enrichment 
                 Enrichment 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                  4 
                 M1H 
                 2.64 
                 2.66 
                 2.65 
                 1.68 
                 1.21 
                 1.44 
               
               
                  5 
                 M1Y 
                 2.56 
                 2.16 
                 2.36 
                 1.18 
                 0.81 
                 1.00 
               
               
                  6 
                 M1W 
                 1.95 
                 1.71 
                 1.83 
                 0.99 
                 0.92 
                 0.96 
               
               
                  7 
                 M1Q 
                 1.23 
                 1.38 
                 1.31 
                 0.58 
                 0.79 
                 0.68 
               
               
                  8 
                 M1T 
                 0.79 
                 0.56 
                 0.68 
                 0.63 
                 0.60 
                 0.61 
               
               
                  9 
                 M1F 
                 0.71 
                 0.90 
                 0.80 
                 0.39 
                 0.33 
                 0.36 
               
               
                  10 
                 M1S 
                 0.55 
                 0.21 
                 0.38 
                 0.27 
                 0.41 
                 0.34 
               
               
                  11 
                 M1I 
                 0.11 
                 0.01 
                 0.06 
                 −0.18 
                 0.19 
                 0.00 
               
               
                  12 
                 M1R 
                 −0.01 
                 0.22 
                 0.10 
                 0.11 
                 0.30 
                 0.21 
               
               
                  13 
                 M1N 
                 −0.37 
                 −0.13 
                 −0.25 
                 −0.04 
                 0.08 
                 0.02 
               
               
                  14 
                 K6R 
                 1.84 
                 1.91 
                 1.87 
                 1.22 
                 0.80 
                 1.01 
               
               
                  15 
                 T7M 
                 2.72 
                 2.68 
                 2.70 
                 1.39 
                 1.32 
                 1.36 
               
               
                  16 
                 T7I 
                 1.70 
                 1.63 
                 1.66 
                 1.04 
                 1.02 
                 1.03 
               
               
                  17 
                 T7C 
                 1.16 
                 1.23 
                 1.20 
                 0.90 
                 0.69 
                 0.79 
               
               
                  18 
                 T7L 
                 0.62 
                 0.61 
                 0.61 
                 −0.07 
                 −0.31 
                 −0.19 
               
               
                  19 
                 T7V 
                 0.24 
                 0.42 
                 0.33 
                 −0.04 
                 0.09 
                 0.02 
               
               
                  20 
                 T9S 
                 0.34 
                 0.47 
                 0.40 
                 0.19 
                 0.17 
                 0.18 
               
               
                  21 
                 T9I 
                 0.30 
                 0.61 
                 0.46 
                 −0.01 
                 0.52 
                 0.25 
               
               
                  22 
                 T9E 
                 0.37 
                 −0.44 
                 −0.04 
                 0.02 
                 0.63 
                 0.33 
               
               
                  23 
                 T9V 
                 0.32 
                 −0.21 
                 0.06 
                 −0.36 
                 0.06 
                 −0.15 
               
               
                  24 
                 T12M 
                 1.09 
                 0.86 
                 0.97 
                 0.65 
                 1.03 
                 0.84 
               
               
                  25 
                 T12Y 
                 0.14 
                 0.36 
                 0.25 
                 0.52 
                 0.17 
                 0.35 
               
               
                  26 
                 I13F 
                 1.04 
                 0.03 
                 0.53 
                 −0.26 
                 0.07 
                 −0.09 
               
               
                  27 
                 I13H 
                 −0.27 
                 1.28 
                 0.50 
                 −2.48 
                 −0.71 
                 −1.60 
               
               
                  28 
                 I13P 
                 2.15 
                 −0.99 
                 0.58 
                 −3.84 
                 −4.62 
                 −4.23 
               
               
                  29 
                 T14D 
                 2.90 
                 2.86 
                 2.88 
                 1.65 
                 1.40 
                 1.53 
               
               
                  30 
                 T14E 
                 2.88 
                 2.88 
                 2.88 
                 1.84 
                 1.54 
                 1.69 
               
               
                  31 
                 T14N 
                 2.84 
                 2.69 
                 2.76 
                 1.50 
                 1.37 
                 1.44 
               
               
                  32 
                 T14H 
                 2.63 
                 2.50 
                 2.56 
                 1.47 
                 1.84 
                 1.65 
               
               
                  33 
                 E16T 
                 1.12 
                 0.64 
                 0.88 
                 0.27 
                 0.33 
                 0.30 
               
               
                  34 
                 E16M 
                 0.71 
                 0.45 
                 0.58 
                 0.93 
                 0.55 
                 0.74 
               
               
                  35 
                 E16Y 
                 0.23 
                 0.47 
                 0.35 
                 −0.32 
                 −0.11 
                 −0.22 
               
               
                  36 
                 E16H 
                 0.00 
                 0.05 
                 0.03 
                 −0.21 
                 −0.05 
                 −0.13 
               
               
                  37 
                 E16N 
                 −0.32 
                 0.54 
                 0.11 
                 0.34 
                 0.18 
                 0.26 
               
               
                  38 
                 E16D 
                 −0.69 
                 0.30 
                 −0.20 
                 0.42 
                 −0.15 
                 0.13 
               
               
                  39 
                 V17C 
                 −2.27 
                 0.12 
                 −1.08 
                 0.60 
                 −0.23 
                 0.19 
               
               
                  40 
                 E18Y 
                 1.28 
                 0.17 
                 0.72 
                 1.20 
                 0.44 
                 0.82 
               
               
                  41 
                 E18M 
                 1.08 
                 0.80 
                 0.94 
                 0.79 
                 0.65 
                 0.72 
               
               
                  42 
                 E18Q 
                 0.61 
                 0.83 
                 0.72 
                 0.02 
                 0.05 
                 0.03 
               
               
                  43 
                 E18H 
                 0.39 
                 1.96 
                 1.17 
                 0.24 
                 −0.11 
                 0.07 
               
               
                  44 
                 E18F 
                 0.09 
                 0.93 
                 0.51 
                 0.22 
                 0.87 
                 0.54 
               
               
                  45 
                 E18W 
                 0.06 
                 0.31 
                 0.18 
                 0.80 
                 0.69 
                 0.75 
               
               
                  46 
                 E18L 
                 0.75 
                 0.76 
                 0.75 
                 0.62 
                 0.71 
                 0.66 
               
               
                  47 
                 E18S 
                 0.47 
                 0.49 
                 0.48 
                 −0.01 
                 0.67 
                 0.33 
               
               
                  48 
                 E18R 
                 −1.22 
                 −0.28 
                 −0.75 
                 −0.24 
                 0.36 
                 0.06 
               
               
                  49 
                 E18T 
                 −0.33 
                 −0.21 
                 −0.27 
                 0.23 
                 0.34 
                 0.28 
               
               
                  50 
                 E18N 
                 0.34 
                 −1.23 
                 −0.45 
                 −0.64 
                 0.85 
                 0.10 
               
               
                  51 
                 E18D 
                 −0.69 
                 −1.02 
                 −0.85 
                 1.65 
                 −1.37 
                 0.14 
               
               
                  52 
                 E18C 
                 −0.06 
                 0.52 
                 0.23 
                 −0.82 
                 0.14 
                 −0.34 
               
               
                  53 
                 P19K 
                 2.12 
                 −0.12 
                 1.00 
                 −0.26 
                 −1.10 
                 −0.68 
               
               
                  54 
                 S20A 
                 0.30 
                 −0.02 
                 0.14 
                 −0.26 
                 −0.25 
                 −0.26 
               
               
                  55 
                 S20N 
                 0.00 
                 0.61 
                 0.31 
                 0.12 
                 −0.61 
                 −0.25 
               
               
                  56 
                 S20D 
                 −0.14 
                 −0.72 
                 −0.43 
                 0.83 
                 0.18 
                 0.50 
               
               
                  57 
                 S20C 
                 −0.36 
                 0.20 
                 −0.08 
                 −0.10 
                 0.21 
                 0.05 
               
               
                  58 
                 S20W 
                 −0.97 
                 0.25 
                 −0.36 
                 0.10 
                 0.24 
                 0.17 
               
               
                  59 
                 D21E 
                 0.20 
                 0.82 
                 0.51 
                 −0.63 
                 0.16 
                 −0.23 
               
               
                  60 
                 N25C 
                 0.45 
                 −0.19 
                 0.13 
                 0.31 
                 0.21 
                 0.26 
               
               
                  61 
                 N25G 
                 1.06 
                 0.35 
                 0.71 
                 0.57 
                 0.19 
                 0.38 
               
               
                  62 
                 N25I 
                 0.85 
                 0.81 
                 0.83 
                 0.41 
                 0.39 
                 0.40 
               
               
                  63 
                 N25T 
                 −0.19 
                 0.44 
                 0.12 
                 0.33 
                 0.24 
                 0.29 
               
               
                  64 
                 N25V 
                 0.76 
                 1.01 
                 0.89 
                 0.58 
                 0.56 
                 0.57 
               
               
                  65 
                 N25M 
                 0.50 
                 0.50 
                 0.50 
                 0.46 
                 0.43 
                 0.45 
               
               
                  66 
                 N25L 
                 0.47 
                 0.39 
                 0.43 
                 0.26 
                 0.19 
                 0.23 
               
               
                  67 
                 N25F 
                 0.42 
                 0.34 
                 0.38 
                 0.12 
                 −0.20 
                 −0.04 
               
               
                  68 
                 N25E 
                 0.28 
                 0.84 
                 0.56 
                 0.61 
                 0.64 
                 0.62 
               
               
                  69 
                 N25R 
                 0.25 
                 0.36 
                 0.31 
                 0.25 
                 0.34 
                 0.29 
               
               
                  70 
                 N25Q 
                 0.24 
                 1.21 
                 0.72 
                 0.15 
                 0.22 
                 0.18 
               
               
                  71 
                 N25S 
                 0.18 
                 0.34 
                 0.26 
                 0.27 
                 0.38 
                 0.33 
               
               
                  72 
                 N25A 
                 0.12 
                 0.49 
                 0.30 
                 0.39 
                 0.40 
                 0.40 
               
               
                  73 
                 N25D 
                 0.11 
                 0.54 
                 0.33 
                 0.49 
                 0.41 
                 0.45 
               
               
                  74 
                 N25K 
                 0.11 
                 0.54 
                 0.32 
                 0.17 
                 0.24 
                 0.21 
               
               
                  75 
                 V26I 
                 0.99 
                 1.25 
                 1.12 
                 0.96 
                 0.70 
                 0.83 
               
               
                  76 
                 V26L 
                 0.40 
                 0.69 
                 0.55 
                 0.52 
                 0.58 
                 0.55 
               
               
                  77 
                 A28D 
                 −0.25 
                 0.29 
                 0.02 
                 −0.24 
                 −0.58 
                 −0.41 
               
               
                  78 
                 A28I 
                 −0.45 
                 0.56 
                 0.05 
                 0.37 
                 −0.28 
                 0.05 
               
               
                  79 
                 A28M 
                 0.49 
                 −0.42 
                 0.03 
                 −0.51 
                 0.12 
                 −0.19 
               
               
                  80 
                 A28W 
                 0.48 
                 −0.41 
                 0.03 
                 0.41 
                 −0.17 
                 0.12 
               
               
                  81 
                 A28Q 
                 0.47 
                 0.42 
                 0.44 
                 0.59 
                 −0.31 
                 0.14 
               
               
                  82 
                 A28E 
                 0.23 
                 0.40 
                 0.31 
                 0.53 
                 0.32 
                 0.42 
               
               
                  83 
                 K29M 
                 1.72 
                 1.48 
                 1.60 
                 1.23 
                 0.57 
                 0.90 
               
               
                  84 
                 K29H 
                 0.02 
                 0.32 
                 0.17 
                 −3.26 
                 −2.20 
                 −2.73 
               
               
                  85 
                 K29L 
                 0.15 
                 0.07 
                 0.11 
                 0.21 
                 0.30 
                 0.26 
               
               
                  86 
                 K29R 
                 −0.07 
                 0.05 
                 −0.01 
                 0.45 
                 0.10 
                 0.28 
               
               
                  87 
                 K29Q 
                 −0.21 
                 0.31 
                 0.05 
                 −0.26 
                 0.17 
                 −0.04 
               
               
                  88 
                 Q31C 
                 1.49 
                 0.88 
                 1.19 
                 1.49 
                 1.66 
                 1.57 
               
               
                  89 
                 Q31W 
                 0.97 
                 1.26 
                 1.11 
                 0.71 
                 0.70 
                 0.70 
               
               
                  90 
                 Q31R 
                 0.66 
                 −0.37 
                 0.15 
                 0.14 
                 −0.58 
                 −0.22 
               
               
                  91 
                 Q31H 
                 0.66 
                 −0.29 
                 0.19 
                 0.13 
                 0.09 
                 0.11 
               
               
                  92 
                 Q31M 
                 −0.84 
                 −2.63 
                 −1.74 
                 −0.05 
                 0.18 
                 0.07 
               
               
                  93 
                 Q31F 
                 0.95 
                 1.39 
                 1.17 
                 1.04 
                 1.14 
                 1.09 
               
               
                  94 
                 Q31L 
                 0.71 
                 0.11 
                 0.41 
                 −0.23 
                 −0.06 
                 −0.15 
               
               
                  95 
                 Q31Y 
                 0.31 
                 0.34 
                 0.32 
                 −0.26 
                 0.16 
                 −0.05 
               
               
                  96 
                 D32R 
                 0.61 
                 −0.54 
                 0.03 
                 −0.53 
                 −0.39 
                 −0.46 
               
               
                  97 
                 D32E 
                 0.41 
                 0.00 
                 0.21 
                 −0.11 
                 −0.28 
                 −0.19 
               
               
                  98 
                 D32A 
                 0.20 
                 0.02 
                 0.11 
                 0.19 
                 0.19 
                 0.19 
               
               
                  99 
                 K33H 
                 4.03 
                 3.45 
                 3.74 
                 1.71 
                 1.56 
                 1.64 
               
               
                 100 
                 K33A 
                 3.01 
                 3.41 
                 3.21 
                 1.73 
                 1.25 
                 1.49 
               
               
                 101 
                 K33C 
                 2.85 
                 1.07 
                 1.96 
                 0.55 
                 0.99 
                 0.77 
               
               
                 102 
                 K33E 
                 2.38 
                 3.03 
                 2.71 
                 1.48 
                 1.05 
                 1.27 
               
               
                 103 
                 K33I 
                 1.91 
                 2.14 
                 2.03 
                 1.32 
                 0.50 
                 0.91 
               
               
                 104 
                 K33Q 
                 3.03 
                 2.77 
                 2.90 
                 1.96 
                 0.99 
                 1.48 
               
               
                 105 
                 K33S 
                 2.84 
                 3.22 
                 3.03 
                 1.34 
                 1.10 
                 1.22 
               
               
                 106 
                 K33V 
                 2.71 
                 2.19 
                 2.45 
                 2.03 
                 1.46 
                 1.75 
               
               
                 107 
                 K33L 
                 2.40 
                 2.65 
                 2.53 
                 1.67 
                 1.53 
                 1.60 
               
               
                 108 
                 K33M 
                 2.30 
                 2.37 
                 2.34 
                 1.51 
                 0.61 
                 1.06 
               
               
                 109 
                 K33T 
                 1.90 
                 1.63 
                 1.77 
                 1.48 
                 1.34 
                 1.41 
               
               
                 110 
                 K33R 
                 0.73 
                 0.10 
                 0.42 
                 0.64 
                 0.10 
                 0.37 
               
               
                 111 
                 K33F 
                 1.91 
                 1.62 
                 1.77 
                 1.16 
                 0.87 
                 1.02 
               
               
                 112 
                 K33Y 
                 0.63 
                 1.41 
                 1.02 
                 1.03 
                 0.94 
                 0.98 
               
               
                 113 
                 K33N 
                 0.48 
                 0.10 
                 0.29 
                 0.06 
                 0.41 
                 0.24 
               
               
                 114 
                 K33W 
                 −2.04 
                 0.01 
                 −1.01 
                 0.35 
                 −0.06 
                 0.15 
               
               
                 115 
                 E34T 
                 2.15 
                 −1.91 
                 0.12 
                 −3.26 
                 −3.83 
                 −3.54 
               
               
                 116 
                 P38L 
                 1.79 
                 1.84 
                 1.81 
                 1.24 
                 1.01 
                 1.13 
               
               
                 117 
                 P38V 
                 1.16 
                 1.26 
                 1.21 
                 0.38 
                 −0.29 
                 0.05 
               
               
                 118 
                 P38S 
                 0.19 
                 0.25 
                 0.22 
                 0.56 
                 −0.02 
                 0.27 
               
               
                 119 
                 P38T 
                 0.95 
                 −0.72 
                 0.11 
                 1.27 
                 0.58 
                 0.92 
               
               
                 120 
                 P38C 
                 1.21 
                 1.83 
                 1.52 
                 0.46 
                 0.57 
                 0.52 
               
               
                 121 
                 P38F 
                 0.91 
                 0.43 
                 0.67 
                 0.48 
                 1.13 
                 0.81 
               
               
                 122 
                 P38W 
                 0.61 
                 0.60 
                 0.60 
                 −0.87 
                 −0.32 
                 −0.59 
               
               
                 123 
                 P38I 
                 0.40 
                 0.92 
                 0.66 
                 1.62 
                 −0.34 
                 0.64 
               
               
                 124 
                 P38A 
                 −0.41 
                 0.53 
                 0.06 
                 −0.16 
                 0.15 
                 0.00 
               
               
                 125 
                 P38N 
                 2.28 
                 −0.25 
                 1.02 
                 −1.91 
                 −1.07 
                 −1.49 
               
               
                 126 
                 P38Q 
                 0.85 
                 −1.35 
                 −0.25 
                 1.18 
                 −0.17 
                 0.50 
               
               
                 127 
                 P38H 
                 0.87 
                 −0.35 
                 0.26 
                 0.33 
                 0.83 
                 0.58 
               
               
                 128 
                 P38K 
                 −2.14 
                 0.10 
                 −1.02 
                 −0.61 
                 1.11 
                 0.25 
               
               
                 129 
                 P38M 
                 −2.07 
                 1.45 
                 −0.31 
                 1.37 
                 1.13 
                 1.25 
               
               
                 130 
                 P38Y 
                 1.79 
                 −0.30 
                 0.74 
                 0.66 
                 −0.03 
                 0.31 
               
               
                 131 
                 D39Q 
                 −3.10 
                 −2.46 
                 −2.78 
                 −0.45 
                 0.51 
                 0.03 
               
               
                 132 
                 D39G 
                 −0.20 
                 −0.22 
                 −0.21 
                 0.60 
                 −0.49 
                 0.06 
               
               
                 133 
                 D39L 
                 0.49 
                 −0.38 
                 0.06 
                 −2.90 
                 −1.42 
                 −2.16 
               
               
                 134 
                 D39S 
                 −2.04 
                 −0.99 
                 −1.51 
                 0.16 
                 −0.08 
                 0.04 
               
               
                 135 
                 D39W 
                 0.90 
                 1.09 
                 0.99 
                 0.86 
                 −0.85 
                 0.00 
               
               
                 136 
                 D39E 
                 0.29 
                 0.44 
                 0.36 
                 −0.14 
                 −0.23 
                 −0.18 
               
               
                 137 
                 Q40D 
                 0.13 
                 −0.75 
                 −0.31 
                 1.27 
                 0.33 
                 0.80 
               
               
                 138 
                 Q40E 
                 1.67 
                 1.08 
                 1.37 
                 1.84 
                 0.52 
                 1.18 
               
               
                 139 
                 Q41V 
                 −0.37 
                 0.10 
                 −0.14 
                 0.13 
                 −0.03 
                 0.05 
               
               
                 140 
                 Q41Y 
                 0.73 
                 0.64 
                 0.68 
                 0.40 
                 0.53 
                 0.47 
               
               
                 141 
                 Q41I 
                 0.30 
                 0.30 
                 0.30 
                 0.08 
                 −0.25 
                 −0.08 
               
               
                 142 
                 Q41C 
                 0.22 
                 0.13 
                 0.18 
                 −0.05 
                 0.00 
                 −0.03 
               
               
                 143 
                 R42S 
                 −0.13 
                 −0.02 
                 −0.08 
                 0.05 
                 0.41 
                 0.23 
               
               
                 144 
                 R42H 
                 2.18 
                 1.89 
                 2.04 
                 0.89 
                 1.16 
                 1.03 
               
               
                 145 
                 R42F 
                 1.99 
                 1.77 
                 1.88 
                 1.40 
                 1.13 
                 1.26 
               
               
                 146 
                 R42W 
                 1.99 
                 2.14 
                 2.06 
                 1.90 
                 1.09 
                 1.50 
               
               
                 147 
                 R42Y 
                 1.44 
                 1.69 
                 1.57 
                 1.13 
                 1.26 
                 1.19 
               
               
                 148 
                 R42N 
                 1.18 
                 1.05 
                 1.12 
                 1.34 
                 0.68 
                 1.01 
               
               
                 149 
                 R42C 
                 0.37 
                 0.47 
                 0.42 
                 0.54 
                 0.01 
                 0.28 
               
               
                 150 
                 A44T 
                 1.70 
                 0.87 
                 1.28 
                 0.75 
                 0.59 
                 0.67 
               
               
                 151 
                 A46Q 
                 3.60 
                 3.22 
                 3.41 
                 1.30 
                 1.65 
                 1.47 
               
               
                 
                   152 
                 
                 
                   A46G 
                 
                 0.48 
                 0.72 
                 0.60 
                 1.37 
                 −1.71 
                 −0.17 
               
               
                 153 
                 K48N 
                 −0.15 
                 0.08 
                 −0.04 
                 0.09 
                 0.03 
                 0.06 
               
               
                 154 
                 K48T 
                 1.20 
                 1.08 
                 1.14 
                 0.84 
                 0.66 
                 0.75 
               
               
                 
                   155 
                 
                 
                   K48M 
                 
                 0.87 
                 0.94 
                 0.91 
                 0.63 
                 0.70 
                 0.67 
               
               
                 156 
                 K48V 
                 0.59 
                 0.48 
                 0.54 
                 0.23 
                 0.46 
                 0.34 
               
               
                 157 
                 K48Q 
                 0.59 
                 0.51 
                 0.55 
                 0.20 
                 0.47 
                 0.34 
               
               
                 158 
                 K48I 
                 0.50 
                 0.77 
                 0.64 
                 0.35 
                 0.49 
                 0.42 
               
               
                 159 
                 K48R 
                 0.39 
                 0.32 
                 0.35 
                 0.09 
                 0.19 
                 0.14 
               
               
                 160 
                 K48L 
                 0.05 
                 0.04 
                 0.05 
                 0.28 
                 0.12 
                 0.20 
               
               
                 161 
                 S49M 
                 1.00 
                 0.69 
                 0.84 
                 0.57 
                 0.98 
                 0.77 
               
               
                 162 
                 S49C 
                 0.95 
                 0.24 
                 0.60 
                 −0.04 
                 −0.31 
                 −0.18 
               
               
                 163 
                 S49L 
                 0.85 
                 1.15 
                 1.00 
                 0.97 
                 0.80 
                 0.88 
               
               
                 164 
                 S49V 
                 0.80 
                 0.23 
                 0.52 
                 0.52 
                 0.45 
                 0.49 
               
               
                 165 
                 S49P 
                 0.65 
                 0.91 
                 0.78 
                 0.71 
                 0.44 
                 0.58 
               
               
                 166 
                 S49A 
                 0.62 
                 0.39 
                 0.50 
                 0.76 
                 0.06 
                 0.41 
               
               
                 167 
                 S49I 
                 0.04 
                 0.56 
                 0.30 
                 0.60 
                 −0.26 
                 0.17 
               
               
                 168 
                 S49N 
                 0.31 
                 −0.23 
                 0.04 
                 −0.59 
                 −0.11 
                 −0.35 
               
               
                 169 
                 S49G 
                 0.27 
                 −0.23 
                 0.02 
                 −0.57 
                 −0.05 
                 −0.31 
               
               
                 170 
                 S49E 
                 0.84 
                 0.04 
                 0.44 
                 0.96 
                 0.67 
                 0.81 
               
               
                 
                   171 
                 
                 
                   S49D 
                 
                 0.11 
                 0.71 
                 0.41 
                 0.39 
                 0.49 
                 0.44 
               
               
                 172 
                 E51D 
                 0.31 
                 0.72 
                 0.52 
                 0.29 
                 0.92 
                 0.61 
               
               
                 173 
                 D52E 
                 0.43 
                 0.14 
                 0.28 
                 −0.30 
                 0.37 
                 0.04 
               
               
                 174 
                 R54N 
                 −0.26 
                 0.65 
                 0.19 
                 0.03 
                 −0.01 
                 0.01 
               
               
                 175 
                 R54C 
                 0.24 
                 −0.12 
                 0.06 
                 −0.43 
                 0.29 
                 −0.07 
               
               
                 176 
                 R54Q 
                 −0.05 
                 0.32 
                 0.14 
                 −0.04 
                 0.00 
                 −0.02 
               
               
                 177 
                 R54F 
                 1.01 
                 0.52 
                 0.76 
                 0.66 
                 0.43 
                 0.55 
               
               
                 178 
                 R54Y 
                 0.92 
                 0.90 
                 0.91 
                 0.78 
                 0.71 
                 0.75 
               
               
                 179 
                 R54M 
                 0.82 
                 0.89 
                 0.85 
                 0.56 
                 0.55 
                 0.56 
               
               
                 180 
                 R54H 
                 0.78 
                 0.96 
                 0.87 
                 0.43 
                 0.55 
                 0.49 
               
               
                 181 
                 R54T 
                 0.62 
                 0.71 
                 0.66 
                 0.76 
                 0.64 
                 0.70 
               
               
                 182 
                 R54K 
                 0.07 
                 0.54 
                 0.30 
                 −0.16 
                 −0.29 
                 −0.22 
               
               
                 183 
                 T55R 
                 0.11 
                 −0.22 
                 −0.06 
                 0.19 
                 0.05 
                 0.12 
               
               
                 184 
                 S57N 
                 1.72 
                 0.96 
                 1.34 
                 0.82 
                 0.56 
                 0.69 
               
               
                 185 
                 S57G 
                 1.70 
                 1.63 
                 1.66 
                 1.24 
                 0.85 
                 1.05 
               
               
                 186 
                 S57D 
                 1.05 
                 1.39 
                 1.22 
                 0.89 
                 0.83 
                 0.86 
               
               
                 187 
                 S57H 
                 0.54 
                 0.90 
                 0.72 
                 −0.04 
                 0.39 
                 0.17 
               
               
                 188 
                 S57A 
                 0.29 
                 0.46 
                 0.37 
                 −0.03 
                 0.08 
                 0.03 
               
               
                 189 
                 S57E 
                 0.28 
                 0.62 
                 0.45 
                 0.42 
                 0.28 
                 0.35 
               
               
                 190 
                 S57Q 
                 0.07 
                 0.07 
                 0.07 
                 0.27 
                 0.01 
                 0.14 
               
               
                 191 
                 S57R 
                 0.05 
                 −0.01 
                 0.02 
                 −0.32 
                 −0.03 
                 −0.18 
               
               
                 192 
                 S57K 
                 −0.22 
                 −0.58 
                 −0.40 
                 −0.07 
                 0.36 
                 0.15 
               
               
                 193 
                 S57M 
                 −0.11 
                 0.12 
                 0.01 
                 −0.17 
                 0.09 
                 −0.04 
               
               
                 194 
                 D58S 
                 0.29 
                 0.38 
                 0.33 
                 0.24 
                 0.17 
                 0.21 
               
               
                 195 
                 N60E 
                 0.90 
                 0.43 
                 0.66 
                 0.13 
                 0.38 
                 0.25 
               
               
                 196 
                 N60Q 
                 0.13 
                 0.03 
                 0.08 
                 0.01 
                 0.58 
                 0.29 
               
               
                 197 
                 I61L 
                 1.10 
                 1.02 
                 1.06 
                 0.75 
                 0.59 
                 0.67 
               
               
                 198 
                 K63M 
                 −0.24 
                 0.09 
                 −0.07 
                 0.18 
                 0.22 
                 0.20 
               
               
                 199 
                 K63F 
                 −0.01 
                 −0.02 
                 −0.02 
                 −0.06 
                 0.22 
                 0.08 
               
               
                 200 
                 K63V 
                 −0.15 
                 −0.11 
                 −0.13 
                 0.00 
                 0.02 
                 0.01 
               
               
                 201 
                 K63I 
                 1.39 
                 1.20 
                 1.29 
                 0.87 
                 0.70 
                 0.78 
               
               
                 202 
                 S65P 
                 3.41 
                 2.89 
                 3.15 
                 1.91 
                 1.41 
                 1.66 
               
               
                 203 
                 S65K 
                 1.61 
                 1.69 
                 1.65 
                 0.74 
                 0.53 
                 0.63 
               
               
                 204 
                 S65A 
                 1.29 
                 1.01 
                 1.15 
                 1.17 
                 0.73 
                 0.95 
               
               
                 205 
                 S65E 
                 1.29 
                 1.79 
                 1.54 
                 0.74 
                 0.85 
                 0.80 
               
               
                 206 
                 S65R 
                 1.15 
                 1.13 
                 1.14 
                 1.48 
                 0.72 
                 1.10 
               
               
                 207 
                 S65Q 
                 −0.02 
                 0.02 
                 0.00 
                 0.01 
                 0.24 
                 0.12 
               
               
                 208 
                 S65H 
                 0.69 
                 1.50 
                 1.10 
                 1.34 
                 0.98 
                 1.16 
               
               
                 209 
                 S65N 
                 0.04 
                 0.71 
                 0.38 
                 0.71 
                 −0.04 
                 0.34 
               
               
                 210 
                 S65D 
                 0.02 
                 0.70 
                 0.36 
                 0.61 
                 1.10 
                 0.85 
               
               
                 211 
                 K66R 
                 −0.84 
                 −0.54 
                 −0.69 
                 0.13 
                 0.97 
                 0.55 
               
               
                 212 
                 L67C 
                 −0.31 
                 0.39 
                 0.04 
                 0.96 
                 0.50 
                 0.73 
               
               
                 213 
                 L67Y 
                 −0.58 
                 0.86 
                 0.14 
                 0.50 
                 0.65 
                 0.58 
               
               
                 214 
                 L67H 
                 0.84 
                 1.71 
                 1.27 
                 3.20 
                 1.87 
                 2.54 
               
               
                 215 
                 L67T 
                 0.69 
                 −0.52 
                 0.09 
                 −0.30 
                 0.52 
                 0.11 
               
               
                 
                   216 
                 
                 
                   L67K 
                 
                 2.08 
                 1.93 
                 2.01 
                 1.40 
                 0.39 
                 0.89 
               
               
                 217 
                 L67R 
                 1.43 
                 1.74 
                 1.59 
                 1.05 
                 0.76 
                 0.90 
               
               
                 218 
                 L67S 
                 1.15 
                 1.20 
                 1.18 
                 1.24 
                 0.63 
                 0.94 
               
               
                 219 
                 L67M 
                 0.98 
                 1.07 
                 1.03 
                 0.65 
                 0.88 
                 0.77 
               
               
                 220 
                 H68E 
                 −0.69 
                 −1.48 
                 −1.08 
                 0.33 
                 −0.13 
                 0.10 
               
               
                 221 
                 H68M 
                 2.53 
                 2.04 
                 2.28 
                 0.99 
                 1.58 
                 1.28 
               
               
                 222 
                 H68Q 
                 0.44 
                 −0.30 
                 0.07 
                 −0.62 
                 −0.38 
                 −0.50 
               
               
                 223 
                 P69R 
                 −2.27 
                 −1.59 
                 −1.93 
                 0.20 
                 1.29 
                 0.75 
               
               
                 224 
                 L73M 
                 2.69 
                 2.52 
                 2.61 
                 1.58 
                 1.28 
                 1.43 
               
               
                 225 
                 R74Q 
                 2.60 
                 1.98 
                 2.29 
                 1.56 
                 1.52 
                 1.54 
               
               
                 226 
                 R74V 
                 1.58 
                 1.44 
                 1.51 
                 1.19 
                 0.70 
                 0.95 
               
               
                 
                   227 
                 
                 
                   R74L 
                 
                 1.35 
                 0.88 
                 1.11 
                 0.95 
                 0.76 
                 0.85 
               
               
                 228 
                 R74M 
                 1.16 
                 0.91 
                 1.04 
                 0.68 
                 0.68 
                 0.68 
               
               
                 229 
                 R74I 
                 0.84 
                 0.83 
                 0.83 
                 0.63 
                 0.58 
                 0.61 
               
               
                 230 
                 R74C 
                 0.64 
                 0.99 
                 0.81 
                 0.68 
                 0.63 
                 0.65 
               
               
                 231 
                 R74E 
                 0.53 
                 0.23 
                 0.38 
                 0.14 
                 −0.35 
                 −0.10 
               
               
                 232 
                 R74T 
                 0.40 
                 0.26 
                 0.33 
                 0.25 
                 0.38 
                 0.31 
               
               
                 233 
                 R74K 
                 0.04 
                 0.21 
                 0.12 
                 0.10 
                 0.17 
                 0.13 
               
               
                   
               
               
                 *The amino acid substitutions highlighted in underlined, gray are also disclosed in WO2017132746A1 and are excluded as claimed subject matter herein to the extent that Ubvs that include all these amino acid substitutions (i.e., as SEQ ID NOS: 2 or 3). The reported amino acid substitutions are presented in the polypeptide amino acid sequence background of SEQ ID NO: 2 in the context of a fusion protein that includes one of the adenylate cyclase fragments. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Individual screen of amino acid changes a   
               
            
           
           
               
               
            
               
                   
                 Dunnett&#39;s Multiple Comparison 
               
            
           
           
               
               
               
               
               
            
               
                 SEQ ID 
                 A.A. 
                 Percent GFP Positive 
                   
                 Adjusted 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 NO: 
                 change 
                 Rep 1 
                 Rep 2 
                 Rep 3 
                 Comparison 
                 Summary 
                 P Value 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 3 
                 None (WT) 
                 9 
                 7.6 
                 11.8 
                   
                   
                   
               
               
                 4 
                 M1H 
                 41.2 
                 41.5 
                 47.8 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + M1H 
               
               
                 14 
                 K6R 
                 19.8 
                 27 
                 24 
                 i53 vs. 
                 ** 
                 0.0029 
               
               
                   
                   
                   
                   
                   
                 i53 + K6R 
               
               
                 15 
                 T7M 
                 32.8 
                 33.3 
                 36.8 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + T7M 
               
               
                 30 
                 T14E 
                 43.5 
                 38.7 
                 46.7 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + T14E 
               
               
                 75 
                 V26I 
                 20.9 
                 14.2 
                 12.3 
                 i53 vs. 
                 ns 
                 0.5657 
               
               
                   
                   
                   
                   
                   
                 i53 + V26I 
               
               
                 83 
                 K29M 
                 23.9 
                 16.5 
                 17.8 
                 i53 vs. 
                 ns 
                 0.0807 
               
               
                   
                   
                   
                   
                   
                 i53 + K29M 
               
               
                 89 
                 Q31W 
                 18.3 
                 8.2 
                 14.6 
                 i53 vs. 
                 ns 
                 0.9499 
               
               
                   
                   
                   
                   
                   
                 i53 + Q31W 
               
               
                 105 
                 K33S 
                 34.8 
                 47 
                 41.8 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + K33S 
               
               
                 99 
                 K33H 
                 48.6 
                 35.9 
                 46 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + K33H 
               
               
                 100 
                 K33A 
                 51.5 
                 45.4 
                 48.1 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + K33A 
               
               
                 116 
                 P38L 
                 28.7 
                 22.1 
                 26.9 
                 i53 vs. 
                 *** 
                 0.0004 
               
               
                   
                   
                   
                   
                   
                 i53 + P38L 
               
               
                 146 
                 R42W 
                 28.7 
                 21.3 
                 24.8 
                 i53 vs. 
                 *** 
                 0.0009 
               
               
                   
                   
                   
                   
                   
                 i53 + R42W 
               
               
                 150 
                 A44T 
                 17.5 
                 7.7 
                 12.8 
                 i53 vs. 
                 ns 
                 0.9941 
               
               
                   
                   
                   
                   
                   
                 i53 + A44T 
               
               
                 151 
                 A46Q 
                 42.6 
                 26.6 
                 39.1 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + A46Q 
               
               
                 154 
                 K48T 
                 16.9 
                 14 
                 14.5 
                 i53 vs. 
                 ns 
                 0.7119 
               
               
                   
                   
                   
                   
                   
                 i53 + K48T 
               
               
                 163 
                 S49L 
                 18.8 
                 13.6 
                 17.7 
                 i53 vs. 
                 ns 
                 0.3845 
               
               
                   
                   
                   
                   
                   
                 i53 + S49L 
               
               
                 178 
                 R54Y 
                 21.4 
                 23.8 
                 20 
                 i53 vs. 
                 * 
                 0.0142 
               
               
                   
                   
                   
                   
                   
                 i53 + R54Y 
               
               
                 185 
                 S57G 
                 31.9 
                 29.9 
                 25.9 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + S57G 
               
               
                 197 
                 I61L 
                 15.9 
                 17.6 
                 17.2 
                 i53 vs. 
                 ns 
                 0.3494 
               
               
                   
                   
                   
                   
                   
                 i53 + I61L 
               
               
                 201 
                 K63I 
                 50.7 
                 50.9 
                 52.6 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + K63I 
               
               
                 202 
                 S65P 
                 45.8 
                 39.5 
                 45.5 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + S65P 
               
               
                 216 
                 L67K 
                 24.2 
                 11.2 
                 21.5 
                 i53 vs. 
                 ns 
                 0.1074 
               
               
                   
                   
                   
                   
                   
                 i53 + L67K 
               
               
                 221 
                 H68M 
                 28.6 
                 23.5 
                 28.3 
                 i53 vs. 
                 *** 
                 0.0002 
               
               
                   
                   
                   
                   
                   
                 i53 + H68M 
               
               
                 224 
                 L73M 
                 36.2 
                 29.2 
                 39.5 
                 i53 vs. 
                 **** 
                 &lt;0.0001 
               
               
                   
                   
                   
                   
                   
                 i53 + L73M 
               
               
                   
               
               
                   a NS means not significant; 
               
               
                 *, **, ***, **** reflects qualitative measure of the strength of association the Ubv has with 53BP1 compared to the similar association of i53 with 53BP1. 
               
            
           
         
       
     
     Example 2. Mutations Identified by the Two-Hybrid Screen Improve the Affinity of i53 for 53BP1 In Vitro 
     In order to assess the effect of mutations identified from the two-hybrid screen on the affinity of the Ubvs for 53BP1, Ubvs consisting of the i53 sequence with an N-terminal His tag and short flexible linker plus individual or combinations of screen-identified mutations were purified from  E. coli  (Table 3). Biolayer interferometry was used to measure the affinity of the purified proteins. Briefly, a purified Ubv was diluted in reaction buffer (1×PBS pH7.4, 0.1 mg/mL BSA, 0.001% Tween 20) to 2 ug/mL. Purified 53BP1 (amino acids 1484-1603) fused to MBP was diluted in reaction buffer to between 20 μM and 10 nM (Table 3, Table 4)). For each Ubv, 8 Ni-NTA sensor tips were hydrated and then loaded with the 2 ug/ml of a Ubv for 30 seconds. Sensor tips were then incubated in reaction buffer for 45 seconds to obtain a baseline. Tips were then moved into either empty buffer or seven different concentrations of purified 53BP1 and the association was measured. Tips were then moved back into reaction buffer and the dissociation was measured. Kon, Koff, and Kd were calculated using a 1:1 binding model using a global fit (Table 4). 
     The effect of individual mutations on the affinity of the Ubv for 53BP1 was found to correlate with the percent reporter positive cells measured from the high throughput screen ( FIG.  4   ). Ubvs containing either four or nine amino acid substitutions relative to the i53 sequence were tested using BLI and were found to have dramatically (5 to 100 fold) improved affinity for the 53BP1 fragment ( FIG.  5 A  and Table 4). A second experiment was performed using CM1 and CM7 using a longer association time (360 seconds) to allow binding to closer approach equilibrium. The BLI response vs 53BP1 fragment concentration was plotted in prism to calculate the Kd using a one site-specific binding nonlinear fit model. An i53 response was plotted on the same graph however the association time used (90 seconds) was shorter due to needing a shorter time to reach equilibrium because of the fast off rate of i53 ( FIG.  5 B ,  FIG.  5 C , Table 4). 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Amino acid and DNA sequences 
               
            
           
           
               
               
               
               
            
               
                   
                 Amino 
                   
                   
               
               
                 Name 
                 acid 
                   
                   
               
               
                 [SEQ ID 
                 changes 
                   
                   
               
               
                 NOS] a   
                 in i53 
                 Protein Sequence 
                 DNA sequence 
               
               
                   
               
               
                 i53 
                 None 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGTGGAT 
               
               
                 [3; 883] 
                   
                 VKTLTGKTITLEVEPS 
                 CTGGCATGTTGATTTTCGTAAAGACGTTG 
               
               
                   
                   
                 DTIENVKAKIQDKEGIP 
                 ACTGGAAAGACTATCACTTTGGAAGTGG 
               
               
                   
                   
                 PDQQRLAFAGKSLED 
                 AGCCTTCCGATACTATCGAGAATGTTAA 
               
               
                   
                   
                 GRTLSDYNILKDSKLH 
                 GGCCAAAATCCAAGATAAGGAAGGGATT 
               
               
                   
                   
                 PLLRLR 
                 CCTCCAGATCAACAACGCCTTGCTTTTGC 
               
               
                   
                   
                   
                 CGGGAAGAGCCTGGAGGACGGTCGCAC 
               
               
                   
                   
                   
                 ACTGTCTGACTATAACATTCTTAAAGATT 
               
               
                   
                   
                   
                 CTAAATTGCATCCACTGCTGCGCTTGCGT 
               
               
                   
               
               
                 i53 DM 
                 P69L, 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGGGGGT 
               
               
                 [234; 884] 
                 L70V 
                 VKTLTGKTITLEVEPS 
                 CGGGCATGTTGATTTTCGTAAAGACGTT 
               
               
                   
                   
                 DTIENVKAKIQDKEGIP 
                 GACTGGAAAGACTATCACTTTGGAAGTG 
               
               
                   
                   
                 PDQQRLAFAGKSLED 
                 GAGCCTTCCGATACTATCGAGAATGTTA 
               
               
                   
                   
                 GRTLSDYNILKDSKLH 
                 AGGCCAAAATCCAAGATAAGGAAGGGA 
               
               
                   
                   
                 LVLRLR 
                 TTCCTCCAGATCAACAACGCCTTGCTTTT 
               
               
                   
                   
                   
                 GCCGGGAAGAGCCTGGAGGACGGTCGC 
               
               
                   
                   
                   
                 ACACTGTCTGACTATAACATTCTTAAAG 
               
               
                   
                   
                   
                 ATTCTAAATTGCATCTGGTTCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 i53 K6R 
                 K6R 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGGGGGT 
               
               
                 [235; 885] 
                   
                 VRTLTGKTITLEVEPSD 
                 CGGGCATGTTGATTTTCGTACGCACGTTG 
               
               
                   
                   
                 TIENVKAKIQDKEGIPP 
                 ACTGGAAAGACTATCACTTTGGAAGTGG 
               
               
                   
                   
                 DQQRLAFAGKSLEDG 
                 AGCCTTCCGATACTATCGAGAATGTTAA 
               
               
                   
                   
                 RTLSDYNILKDSKLHP 
                 GGCCAAAATCCAAGATAAGGAAGGGATT 
               
               
                   
                   
                 LLRLR 
                 CCTCCAGATCAACAACGCCTTGCTTTTGC 
               
               
                   
                   
                   
                 CGGGAAGAGCCTGGAGGACGGTCGCAC 
               
               
                   
                   
                   
                 ACTGTCTGACTATAACATTCTTAAAGATT 
               
               
                   
                   
                   
                 CTAAATTGCATCCACTGCTGCGCTTGCGT 
               
               
                   
               
               
                 i53 T14E 
                 T14E 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGGGGGT 
               
               
                 [236; 886] 
                   
                 VKTLTGKTIELEVEPS 
                 CGGGCATGTTGATTTTCGTAAAGACGTT 
               
               
                   
                   
                 DTIENVKAKIQDKEGIP 
                 GACTGGAAAGACTATCGAGTTGGAAGTG 
               
               
                   
                   
                 PDQQRLAFAGKSLED 
                 GAGCCTTCCGATACTATCGAGAATGTTA 
               
               
                   
                   
                 GRTLSDYNILKDSKLH 
                 AGGCCAAAATCCAAGATAAGGAAGGGA 
               
               
                   
                   
                 PLLRLR 
                 TTCCTCCAGATCAACAACGCCTTGCTTTT 
               
               
                   
                   
                   
                 GCCGGGAAGAGCCTGGAGGACGGTCGC 
               
               
                   
                   
                   
                 ACACTGTCTGACTATAACATTCTTAAAG 
               
               
                   
                   
                   
                 ATTCTAAATTGCATCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 i53 K33A 
                 K33A 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGGGGGT 
               
               
                 [237; 887] 
                   
                 VKTLTGKTITLEVEPS 
                 CGGGCATGTTGATTTTCGTAAAGACGTT 
               
               
                   
                   
                 DTIENVKAKIQDAEGIP 
                 GACTGGAAAGACTATCACTTTGGAAGTG 
               
               
                   
                   
                 PDQQRLAFAGKSLED 
                 GAGCCTTCCGATACTATCGAGAATGTTA 
               
               
                   
                   
                 GRTLSDYNILKDSKLH 
                 AGGCCAAAATCCAAGATGCCGAAGGGAT 
               
               
                   
                   
                 PLLRLR 
                 TCCTCCAGATCAACAACGCCTTGCTTTTG 
               
               
                   
                   
                   
                 CCGGGAAGAGCCTGGAGGACGGTCGCAC 
               
               
                   
                   
                   
                 ACTGTCTGACTATAACATTCTTAAAGATT 
               
               
                   
                   
                   
                 CTAAATTGCATCCACTGCTGCGCTTGCGT 
               
               
                   
               
               
                 i53 A46Q 
                 A46Q 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGGGGGT 
               
               
                 [238; 888] 
                   
                 VKTLTGKTITLEVEPS 
                 CGGGCATGTTGATTTTCGTAAAGACGTT 
               
               
                   
                   
                 DTIENVKAKIQDKEGIP 
                 GACTGGAAAGACTATCACTTTGGAAGTG 
               
               
                   
                   
                 PDQQRLAFQGKSLED 
                 GAGCCTTCCGATACTATCGAGAATGTTA 
               
               
                   
                   
                 GRTLSDYNILKDSKLH 
                 AGGCCAAAATCCAAGATAAGGAAGGGA 
               
               
                   
                   
                 PLLRLR 
                 TTCCTCCAGATCAACAACGCCTTGCTTTT 
               
               
                   
                   
                   
                 CAAGGGAAGAGCCTGGAGGACGGTCGC 
               
               
                   
                   
                   
                 ACACTGTCTGACTATAACATTCTTAAAG 
               
               
                   
                   
                   
                 ATTCTAAATTGCATCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 i53 K63I 
                 K63I 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGGGGGT 
               
               
                 [239; 889] 
                   
                 VKTLTGKTITLEVEPS 
                 CGGGCATGTTGATTTTCGTAAAGACGTT 
               
               
                   
                   
                 DTIENVKAKIQDKEGIP 
                 GACTGGAAAGACTATCACTTTGGAAGTG 
               
               
                   
                   
                 PDQQRLAFAGKSLED 
                 GAGCCTTCCGATACTATCGAGAATGTTA 
               
               
                   
                   
                 GRTLSDYNILIDSKLHP 
                 AGGCCAAAATCCAAGATAAGGAAGGGA 
               
               
                   
                   
                 LLRLR 
                 TTCCTCCAGATCAACAACGCCTTGCTTTT 
               
               
                   
                   
                   
                 GCCGGGAAGAGCCTGGAGGACGGTCGC 
               
               
                   
                   
                   
                 ACACTGTCTGACTATAACATTCTTATTGA 
               
               
                   
                   
                   
                 TTCTAAATTGCATCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 i53 S65P 
                 S65P 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGGGGGT 
               
               
                 [240; 890] 
                   
                 VKTLTGKTITLEVEPS 
                 CGGGCATGTTGATTTTCGTAAAGACGTT 
               
               
                   
                   
                 DTIENVKAKIQDKEGIP 
                 GACTGGAAAGACTATCACTTTGGAAGTG 
               
               
                   
                   
                 PDQQRLAFAGKSLED 
                 GAGCCTTCCGATACTATCGAGAATGTTA 
               
               
                   
                   
                 GRTLSDYNILKDPKLH 
                 AGGCCAAAATCCAAGATAAGGAAGGGA 
               
               
                   
                   
                 PLLRLR 
                 TTCCTCCAGATCAACAACGCCTTGCTTTT 
               
               
                   
                   
                   
                 GCCGGGAAGAGCCTGGAGGACGGTCGC 
               
               
                   
                   
                   
                 ACACTGTCTGACTATAACATTCTTAAAG 
               
               
                   
                   
                   
                 ATCCTAAATTGCATCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM1 
                 K6R, 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGTGGAT 
               
               
                 [241; 916] 
                 T7M, 
                 VRMLTGKMIELEVEPS 
                 CTGGCATGTTGATTTTCGTACGCATGTTG 
               
               
                   
                 T12M, 
                 DTIENVKAKIQDKEGIP 
                 ACTGGAAAGATGATCGAGTTGGAAGTGG 
               
               
                   
                 T14E, 
                 PDQQRLAFQGKSLED 
                 AGCCTTCCGATACTATCGAGAATGTTAA 
               
               
                   
                 K33H, 
                 GRTLSDYNILKDPKKM 
                 GGCCAAAATCCAAGATCATGAAGGGATT 
               
               
                   
                 A46Q, 
                 PLLRLR 
                 CCTCCAGATCAACAACGCCTTGCTTTTCA 
               
               
                   
                 S65P, 
                   
                 AGGGAAGAGCCTGGAGGACGGTCGCAC 
               
               
                   
                 L67K, 
                   
                 ACTGTCTGACTATAACATTCTTAAAGATC 
               
               
                   
                 H68M 
                   
                 CTAAAAAGATGCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 CM7 
                 K6R, 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGTGGAT 
               
               
                 [242; 917] 
                 K33H, 
                 VRTLTGKTITLEVEPSD 
                 CTGGCATGTTGATTTTCGTACGCACGTTG 
               
               
                   
                 A46Q, 
                 TIENVKAKIQDHEGIPP 
                 ACTGGAAAGACTATCACTTTGGAAGTGG 
               
               
                   
                 S65P 
                 DQQRLAFQGKSLEDG 
                 AGCCTTCCGATACTATCGAGAATGTTAA 
               
               
                   
                   
                 RTLSDYNILKDPKLHP 
                 GGCCAAAATCCAAGATCATGAAGGGATT 
               
               
                   
                   
                 LLRLR 
                 CCTCCAGATCAACAACGCCTTGCTTTTCA 
               
               
                   
                   
                   
                 AGGGAAGAGCCTGGAGGACGGTCGCAC 
               
               
                   
                   
                   
                 ACTGTCTGACTATAACATTCTTAAAGATC 
               
               
                   
                   
                   
                 CTAAATTGCATCCACTGCTGCGCTTGCGT 
               
               
                   
               
               
                 CM13 
                 T7M, 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGTGGAT 
               
               
                 [243; 918] 
                 T14E, 
                 VKMLTGKTIELEVEPS 
                 CTGGCATGTTGATTTTCGTAAAGATGTTG 
               
               
                   
                 A46Q, 
                 DTIENVKAKIQDKEGIP 
                 ACTGGAAAGACTATCGAGTTGGAAGTGG 
               
               
                   
                 L67K 
                 PDQQRLAFQGKSLED 
                 AGCCTTCCGATACTATCGAGAATGTTAA 
               
               
                   
                   
                 GRTLSDYNILKDSKKH 
                 GGCCAAAATCCAAGATAAGGAAGGGATT 
               
               
                   
                   
                 PLLRLR 
                 CCTCCAGATCAACAACGCCTTGCTTTTCA 
               
               
                   
                   
                   
                 AGGGAAGAGCCTGGAGGACGGTCGCAC 
               
               
                   
                   
                   
                 ACTGTCTGACTATAACATTCTTAAAGATT 
               
               
                   
                   
                   
                 CTAAAAAGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 CM26 
                 T12M, 
                 MHHHHHHGGSGMLIF 
                 ATGCACCATCACCACCACCACGGTGGAT 
               
               
                 [244; 919] 
                 K33H, 
                 VKTLTGKMITLEVEPS 
                 CTGGCATGTTGATTTTCGTAAAGACGTTG 
               
               
                   
                 A46Q, 
                 DTIENVKAKIQDHEGIP 
                 ACTGGAAAGATGATCACTTTGGAAGTGG 
               
               
                   
                 H68M 
                 PDQQRLAFQGKSLED 
                 AGCCTTCCGATACTATCGAGAATGTTAA 
               
               
                   
                   
                 GRTLSDYNILKDSKLM 
                 GGCCAAAATCCAAGATCATGAAGGGATT 
               
               
                   
                   
                 PLLRLR 
                 CCTCCAGATCAACAACGCCTTGCTTTTCA 
               
               
                   
                   
                   
                 AGGGAAGAGCCTGGAGGACGGTCGCAC 
               
               
                   
                   
                   
                 ACTGTCTGACTATAACATTCTTAAAGATT 
               
               
                   
                   
                   
                 CTAAATTGATGCCACTGCTGCGCTTGCGT 
               
               
                   
               
               
                 MBP 
                 N/A 
                 MKIEEGKLVIWINGDK 
                 ATGAAAATCGAAGAAGGTAAACTGGTAA 
               
               
                 tagged 
                   
                 GYNGLAEVGKKFEKD 
                 TCTGGATTAACGGCGATAAAGGCTATAA 
               
               
                 53BP1 
                   
                 TGIKVTVEHPDKLEEK 
                 CGGTCTCGCTGAAGTCGGTAAGAAATTC 
               
               
                 fragment 
                   
                 FPQVAATGDGPDIIFW 
                 GAGAAAGATACCGGAATTAAAGTCACCG 
               
               
                 (a.a. 
                   
                 AHDRFGGYAQSGLLA 
                 TTGAGCATCCGGATAAACTGGAAGAGAA 
               
               
                 1484-1603) 
                   
                 EITPDKAFQDKLYPFT 
                 ATTCCCACAGGTTGCGGCAACTGGCGAT 
               
               
                 [245; 891] 
                   
                 WDAVRYNGKLIAYPI 
                 GGCCCTGACATTATCTTCTGGGCACACG 
               
               
                   
                   
                 AVEALSLIYNKDLLPN 
                 ACCGCTTTGGTGGCTACGCTCAATCTGGC 
               
               
                   
                   
                 PPKTWEEIPALDKELK 
                 CTGTTGGCTGAAATCACCCCGGACAAAG 
               
               
                   
                   
                 AKGKSALMFNLQEPY 
                 CGTTCCAGGACAAGCTGTATCCGTTTACC 
               
               
                   
                   
                 FTWPLIAADGGYAFK 
                 TGGGATGCCGTACGTTACAACGGCAAGC 
               
               
                   
                   
                 YENGKYDIKDVGVDN 
                 TGATTGCTTACCCGATCGCTGTTGAAGCG 
               
               
                   
                   
                 AGAKAGLTFLVDLIKN 
                 TTATCGCTGATTTATAACAAAGATCTGCT 
               
               
                   
                   
                 KHMNADTDYSIAEAA 
                 GCCGAACCCGCCAAAAACCTGGGAAGA 
               
               
                   
                   
                 FNKGETAMTINGPWA 
                 GATCCCGGCGCTGGATAAAGAACTGAAA 
               
               
                   
                   
                 WSNIDTSKVNYGVTV 
                 GCGAAAGGTAAGAGCGCGCTGATGTTCA 
               
               
                   
                   
                 LPTFKGQPSKPFVGVL 
                 ACCTGCAAGAACCGTACTTCACCTGGCC 
               
               
                   
                   
                 SAGINAASPNKELAKE 
                 GCTGATTGCTGCTGACGGGGGTTATGCG 
               
               
                   
                   
                 FLENYLLTDEGLEAVN 
                 TTCAAGTATGAAAACGGCAAGTACGACA 
               
               
                   
                   
                 KDKPLGAVALKSYEE 
                 TTAAAGACGTGGGCGTGGATAACGCTGG 
               
               
                   
                   
                 ELAKDPRIAATMENA 
                 CGCGAAAGCGGGTCTGACCTTCCTGGTT 
               
               
                   
                   
                 QKGEIMPNIPQMSAFW 
                 GACCTGATTAAAAACAAACACATGAATG 
               
               
                   
                   
                 YAVRTAVINAASGRQ 
                 CAGACACCGATTACTCCATCGCAGAAGC 
               
               
                   
                   
                 TVDEALKDAQTNSSSN 
                 TGCCTTTAATAAAGGCGAAACAGCGATG 
               
               
                   
                   
                 NNNNNNNNNLGIEEN 
                 ACCATCAACGGCCCGTGGGCATGGTCCA 
               
               
                   
                   
                 LYFQGHMNSFVGLRV 
                 ACATCGACACCAGCAAAGTGAATTATGG 
               
               
                   
                   
                 VAKWSSNGYFYSGKIT 
                 TGTAACGGTACTGCCGACCTTCAAGGGT 
               
               
                   
                   
                 RDVGAGKYKLLFDDG 
                 CAACCATCCAAACCGTTCGTTGGCGTGC 
               
               
                   
                   
                 YECDVLGKDILLCDPIP 
                 TGAGCGCAGGTATTAACGCCGCCAGTCC 
               
               
                   
                   
                 LDTEVTALSEDEYFSA 
                 GAACAAAGAGCTGGCAAAAGAGTTCCTC 
               
               
                   
                   
                 GVVKGHRKESGELYY 
                 GAAAACTATCTGCTGACTGATGAAGGTC 
               
               
                   
                   
                 SIEKEGQRKWYKRMA 
                 TGGAAGCGGTTAATAAAGACAAACCGCT 
               
               
                   
                   
                 VILSLEQGNRLREQYG 
                 GGGTGCCGTAGCGCTGAAGTCTTACGAG 
               
               
                   
                   
                 LG 
                 GAAGAGTTGGCGAAAGATCCACGTATTG 
               
               
                   
                   
                   
                 CCGCCACTATGGAAAACGCCCAGAAAGG 
               
               
                   
                   
                   
                 TGAAATCATGCCGAACATCCCGCAGATG 
               
               
                   
                   
                   
                 TCCGCTTTCTGGTATGCCGTGCGTACTGC 
               
               
                   
                   
                   
                 GGTGATCAACGCCGCCAGCGGTCGTCAG 
               
               
                   
                   
                   
                 ACTGTCGATGAAGCCCTGAAAGACGCGC 
               
               
                   
                   
                   
                 AGACTAATTCGAGCTCGAACAACAACAA 
               
               
                   
                   
                   
                 CAATAACAATAACAACAACCTCGGGATC 
               
               
                   
                   
                   
                 GAGGAAAATCTGTATTTTCAGGGCCACA 
               
               
                   
                   
                   
                 TGAATAGCTTTGTTGGTCTGCGTGTTGTT 
               
               
                   
                   
                   
                 GCAAAATGGTCAAGCAATGGTTATTTCT 
               
               
                   
                   
                   
                 ACAGCGGCAAAATCACCCGTGATGTTGG 
               
               
                   
                   
                   
                 TGCAGGTAAATACAAACTGCTGTTTGAT 
               
               
                   
                   
                   
                 GATGGTTATGAATGTGATGTGCTGGGCA 
               
               
                   
                   
                   
                 AAGATATTCTGCTGTGTGATCCGATTCCG 
               
               
                   
                   
                   
                 CTGGATACCGAAGTTACCGCACTGAGCG 
               
               
                   
                   
                   
                 AAGATGAATATTTCAGTGCCGGTGTTGTT 
               
               
                   
                   
                   
                 AAAGGCCATCGTAAAGAAAGCGGTGAA 
               
               
                   
                   
                   
                 CTGTATTACAGCATTGAAAAAGAAGGTC 
               
               
                   
                   
                   
                 AGCGCAAATGGTATAAACGTATGGCAGT 
               
               
                   
                   
                   
                 TATTCTGAGCCTGGAACAGGGTAATCGT 
               
               
                   
                   
                   
                 CTGCGTGAACAGTATGGTCTGGGT 
               
               
                   
               
               
                   a The SEQ ID NOS shown in brackets correspond to the protein amino acid SEQ ID NO, followed by the DNA nucleic acid SEQ ID NO. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 BLI Data 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 Concentration 
                   
                   
                   
                   
                   
               
               
                   
                 of 53BP1 (a.a. 
               
               
                   
                 1484-1603) 
               
               
                 Protein 
                 (μM) 
                   
                   
                   
                   
                 Full 
               
               
                 (Ligand) 
                 (Analyte) 
                 Response 
                 KD (M) 
                 kon(1/Ms) 
                 kdis(1/s) 
                 R{circumflex over ( )}2 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 i53 
                 20 
                 0.5736 
                 5.92 ± 0.37E−6 
                 1.50 ± 0.09E4 
                 8.87 ± 0.20E−2 
                 0.9867 
               
               
                   
                 5 
                 0.3399 
                 5.92 ± 0.37E−6 
                 1.50 ± 0.09E4 
                 8.87 ± 0.20E−2 
                 0.9867 
               
               
                   
                 2 
                 0.2205 
                 5.92 ± 0.37E−6 
                 l,50 ± 0.09E4 
                 8.87 ± 0.20E−2 
                 0.9867 
               
               
                   
                 1 
                 0.1258 
                 5.92 ± 0.37E−6 
                 1.50 ± 0.09E4 
                 8.87 ± 0.20E−2 
                 0.9867 
               
               
                   
                 0.5 
                 0.0627 
                 5.92 ± 0.37E−6 
                 1.50 ± 0.09E4 
                 8.87 ± 0.20E−2 
                 0.9867 
               
               
                   
                 0.25 
                 0.0221 
                 5.92 ± 0.37E−6 
                 1.50 ± 0.09E4 
                 8.87 ± 0.20E−2 
                 0.9867 
               
               
                   
                 0.125 
                 0.0006 
                 5.92 ± 0.37E−6 
                 1.50 ± 0.09E4 
                 8.87 ± 0.20E−2 
                 0.9867 
               
            
           
           
               
               
               
               
            
               
                 i53 DM 
                 20 
                 0.068 
                 Response was too low to get a good fit to the data 
               
            
           
           
               
               
               
               
            
               
                   
                 5 
                 0.0231 
                   
               
               
                   
                 2 
                 −0.0028 
               
               
                   
                 1 
                 −0.0087 
               
               
                   
                 0.5 
                 −0.0147 
               
               
                   
                 0.25 
                 −0.0151 
               
               
                   
                 0.125 
                 −0.0083 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 i53 K6R 
                 20 
                 0.6539 
                 3.93 ± 0.23E−6 
                 1.64 ± 0.09E4 
                 6.44 ± 0.16E−2 
                 0.9856 
               
               
                   
                 5 
                 0.4106 
                 3.93 ± 0.23E−6 
                 1.64 ± 0.09E4 
                 6.44 ± 0.16E−2 
                 0.9856 
               
               
                   
                 2 
                 0.2749 
                 3.93 ± 0.23E−6 
                 1.64 ± 0.09E4 
                 6.44 ± 0.16E−2 
                 0.9856 
               
               
                   
                 1 
                 0.1711 
                 3.93 ± 0.23E−6 
                 1.64 ± 0.09E4 
                 6.44 ± 0.16E−2 
                 0.9856 
               
               
                   
                 0.5 
                 0.0908 
                 3.93 ± 0.23E−6 
                 1.64 ± 0.09E4 
                 6.44 ± 0.16E−2 
                 0.9856 
               
               
                   
                 0.25 
                 0.038 
                 3.93 ± 0.23E−6 
                 1.64 ± 0.09E4 
                 6.44 ± 0.16E−2 
                 0.9856 
               
               
                   
                 0.125 
                 0.014 
                 3.93 ± 0.23E−6 
                 1.64 ± 0.09E4 
                 6.44 ± 0.16E−2 
                 0.9856 
               
               
                 i53 T14E 
                 20 
                 0.6662 
                 2.11 ± 0.13E−6 
                 3.33 ± 0.19E4 
                 7.02 ± 0.18E−2 
                 0.9837 
               
               
                   
                 5 
                 0.4617 
                 2.11 ± 0.13E−6 
                 3.33 ± 0.19E4 
                 7.02 ± 0.18E−2 
                 0.9837 
               
               
                   
                 2 
                 0.333 
                 2.11 ± 0.13E−6 
                 3.33 ± 0.19E4 
                 7.02 ± 0.18E−2 
                 0.9837 
               
               
                   
                 1 
                 0.2242 
                 2.11 ± 0.13E−6 
                 3.33 ± 0.19E4 
                 7.02 ± 0.18E−2 
                 0.9837 
               
               
                   
                 0.5 
                 0.1227 
                 2.11 ± 0.13E−6 
                 3.33 ± 0.19E4 
                 7.02 ± 0.18E−2 
                 0.9837 
               
               
                   
                 0.25 
                 0.0571 
                 2.11 ± 0.13E−6 
                 3.33 ± 0.19E4 
                 7.02 ± 0.18E−2 
                 0.9837 
               
               
                   
                 0.125 
                 0.0223 
                 2.11 ± 0.13E−6 
                 3.33 ± 0.19E4 
                 7.02 ± 0.18E−2 
                 0.9837 
               
               
                 i53 K33A 
                 20 
                 0.9597 
                 2.10 ± 0.12E−6 
                 2.95 ± 0.16E4 
                 6.20 ± 0.16E−2 
                 0.9848 
               
               
                   
                 5 
                 0.657 
                 2.10 ± 0.12E−6 
                 2.95 ± 0.16E4 
                 6.20 ± 0.16E−2 
                 0.9848 
               
               
                   
                 2 
                 0.4805 
                 2.10 ± 0.12E−6 
                 2.95 ± 0.16E4 
                 6.20 ± 0.16E−2 
                 0.9848 
               
               
                   
                 1 
                 0.3249 
                 2.10 ± 0.12E−6 
                 2.95 ± 0.16E4 
                 6.20 ± 0.16E−2 
                 0.9848 
               
               
                   
                 0.5 
                 0.1851 
                 2.10 ± 0.12E−6 
                 2.95 ± 0.16E4 
                 6.20 ± 0.16E−2 
                 0.9848 
               
               
                   
                 0.25 
                 0.0935 
                 2.10 ± 0.12E−6 
                 2.95 ± 0.16E4 
                 6.20 ± 0.16E−2 
                 0.9848 
               
               
                   
                 0.125 
                 0.0409 
                 2.10 ± 0.12E−6 
                 2.95 ± 0.16E4 
                 6.20 ± 0.16E−2 
                 0.9848 
               
               
                 i53 A46Q 
                 20 
                 1.0136 
                 2.20 ± 0.13E−6 
                 2.26 ± 0.11E4 
                 4.96 ± 0.14E−2 
                 0.9845 
               
               
                   
                 5 
                 0.6996 
                 2.20 ± 0.13E−6 
                 2.26 ± 0.11E4 
                 4.96 ± 0.14E−2 
                 0.9845 
               
               
                   
                 2 
                 0.5003 
                 2.20 ± 0.13E−6 
                 2.26 ± 0.11E4 
                 4.96 ± 0.14E−2 
                 0.9845 
               
               
                   
                 1 
                 0.3476 
                 2.20 ± 0.13E−6 
                 2.26 ± 0.11E4 
                 4.96 ± 0.14E−2 
                 0.9845 
               
               
                   
                 0.5 
                 0.1936 
                 2.20 ± 0.13E−6 
                 2.26 ± 0.11E4 
                 4.96 ± 0.14E−2 
                 0.9845 
               
               
                   
                 0.25 
                 0.1021 
                 2.20 ± 0.13E−6 
                 2.26 ± 0.11E4 
                 4.96 ± 0.14E−2 
                 0.9845 
               
               
                   
                 0.125 
                 0.0512 
                 2.20 ± 0.13E−6 
                 2.26 ± 0.11E4 
                 4.96 ± 0.14E−2 
                 0.9845 
               
               
                 i53 K63I 
                 20 
                 0.7969 
                 2.87 ± 0.17E−6 
                 1.90 ± 0.10E4 
                 5.46 ± 0.15E−2 
                 0.9854 
               
               
                   
                 5 
                 0.5263 
                 2.87 ± 0.17E−6 
                 1.90 ± 0.10E4 
                 5.46 ± 0.15E−2 
                 0.9854 
               
               
                   
                 2 
                 0.3744 
                 2.87 ± 0.17E−6 
                 1.90 ± 0.10E4 
                 5.46 ± 0.15E−2 
                 0.9854 
               
               
                   
                 1 
                 0.2422 
                 2.87 ± 0.17E−6 
                 1.90 ± 0.10E4 
                 5.46 ± 0.15E−2 
                 0.9854 
               
               
                   
                 0.5 
                 0.1404 
                 2.87 ± 0.17E−6 
                 1.90 ± 0.10E4 
                 5.46 ± 0.15E−2 
                 0.9854 
               
               
                   
                 0.25 
                 0.0623 
                 2.87 ± 0.17E−6 
                 1.90 ± 0.10E4 
                 5.46 ± 0.15E−2 
                 0.9854 
               
               
                   
                 0.125 
                 0.0324 
                 2.87 ± 0.17E−6 
                 1.90 ± 0.10E4 
                 5.46 ± 0.15E−2 
                 0.9854 
               
               
                 i53 S65P 
                 20 
                 0.7157 
                 2.09 ± 0.13E−6 
                 2.46 ± 0.14E4 
                 5.14 ± 0.16E−2 
                 0.9819 
               
               
                   
                 5 
                 0.5076 
                 2.09 ± 0.13E−6 
                 2.46 ± 0.14E4 
                 5.14 ± 0.16E−2 
                 0.9819 
               
               
                   
                 2 
                 0.3612 
                 2.09 ± 0.13E−6 
                 2.46 ± 0.14E4 
                 5.14 ± 0.16E−2 
                 0.9819 
               
               
                   
                 1 
                 0.2516 
                 2.09 ± 0.13E−6 
                 2.46 ± 0.14E4 
                 5.14 ± 0.16E−2 
                 0.9819 
               
               
                   
                 0.5 
                 0.143 
                 2.09 ± 0.13E−6 
                 2.46 ± 0.14E4 
                 5.14 ± 0.16E−2 
                 0.9819 
               
               
                   
                 0.25 
                 0.069 
                 2.09 ± 0.13E−6 
                 2.46 ± 0.14E4 
                 5.14 ± 0.16E−2 
                 0.9819 
               
               
                   
                 0.125 
                 0.0384 
                 2.09 ± 0.13E−6 
                 2.46 ± 0.14E4 
                 5.14 ± 0.16E−2 
                 0.9819 
               
               
                 CM1 
                 5.13 
                 1.3836 
                 2.10 ± 0.03E−8 
                 1.47 ± 0.02E5 
                 3.09 ± 0.02E−3 
                 0.9826 
               
               
                   
                 2.05 
                 1.3075 
                 2.10 ± 0.03E−8 
                 1.47 ± 0.02E5 
                 3.09 ± 0.02E−3 
                 0.9826 
               
               
                   
                 1.03 
                 1.248 
                 2.10 ± 0.03E−8 
                 1.47 ± 0.02E5 
                 3.09 ± 0.02E−3 
                 0.9826 
               
               
                   
                 0.5125 
                 1.0736 
                 2.10 ± 0.03E−8 
                 1.47 ± 0.02E5 
                 3.09 ± 0.02E−3 
                 0.9826 
               
               
                   
                 0.2562 
                 0.8876 
                 2.10 ± 0.03E−8 
                 l,47 ± 0.02E5 
                 3.09 ± 0.02E−3 
                 0.9826 
               
               
                   
                 0.128 
                 0.7242 
                 2.10 ± 0.03E−8 
                 1.47 ± 0.02E5 
                 3.09 ± 0.02E−3 
                 0.9826 
               
               
                 CM7 
                 5.13 
                 1.1444 
                 2.14 ± 0.04E−7 
                 3.33 ± 0.06E4 
                 7.14 ± 0.04E−3 
                 0.984 
               
               
                   
                 2.05 
                 0.9886 
                 2.14 ± 0.04E−7 
                 3.33 ± 0.06E4 
                 7.14 ± 0.04E−3 
                 0.984 
               
               
                   
                 1.03 
                 0.8003 
                 2.14 ± 0.04E−7 
                 3.33 ± 0.06E4 
                 7.14 ± 0.04E−3 
                 0.984 
               
               
                   
                 0.5125 
                 0.5888 
                 2.14 ± 0.04E−7 
                 3.33 ± 0.06E4 
                 7.14 ± 0.04E−3 
                 0.984 
               
               
                   
                 0.2562 
                 0.4015 
                 2.14 ± 0.04E−7 
                 3.33 ± 0.06E4 
                 7.14 ± 0.04E−3 
                 0.984 
               
               
                   
                 0.128 
                 0.2514 
                 2.14 ± 0.04E−7 
                 3.33 ± 0.06E4 
                 7.14 ± 0.04E−3 
                 0.984 
               
               
                 CM13 
                 5.13 
                 1.3261 
                 2.22 ± 0.04E−7 
                 4.07 ± 0.07E4 
                 9.02 ± 0.05E−3 
                 0.9863 
               
               
                   
                 2.05 
                 1.1469 
                 2.22 ± 0.04E−7 
                 4.07 ± 0.07E4 
                 9.02 ± 0.05E−3 
                 0.9863 
               
               
                   
                 1.03 
                 0.9475 
                 2.22 ± 0.04E−7 
                 4.07 ± 0.07E4 
                 9.02 ± 0.05E−3 
                 0.9863 
               
               
                   
                 0.5125 
                 0.6938 
                 2.22 ± 0.04E−7 
                 4.07 ± 0.07E4 
                 9.02 ± 0.05E−3 
                 0.9863 
               
               
                   
                 0.2562 
                 0.4733 
                 2.22 ± 0.04E−7 
                 4.07 ± 0.07E4 
                 9.02 ± 0.05E−3 
                 0.9863 
               
               
                   
                 0.128 
                 0.3065 
                 2.22 ± 0.04E−7 
                 4.07 ± 0.07E4 
                 9.02 ± 0.05E−3 
                 0.9863 
               
               
                 CM26 
                 5.13 
                 1.0663 
                 1.23 ± 0.05E−7 
                 1.36 ± 0.05E5 
                 1.67 ± 0.02E−2 
                 0.9642 
               
               
                   
                 2.05 
                 0.9555 
                 1.23 ± 0.05E−7 
                 1.36 ± 0.05E5 
                 1.67 ± 0.02E−2 
                 0.9642 
               
               
                   
                 1.03 
                 0.821 
                 1.23 ± 0.05E−7 
                 1.36 ± 0.05E5 
                 1.67 ± 0.02E−2 
                 0.9642 
               
               
                   
                 0.5125 
                 0.6303 
                 1.23 ± 0.05E−7 
                 1.36 ± 0.05E5 
                 1.67 ± 0.02E−2 
                 0.9642 
               
               
                   
                 0.2562 
                 0.4422 
                 1.23 ± 0.05E−7 
                 1.36 ± 0.05E5 
                 1.67 ± 0.02E−2 
                 0.9642 
               
               
                   
                 0.128 
                 0.298 
                 1.23 ± 0.05E−7 
                 1.36 ± 0.05E5 
                 1.67 ± 0.02E−2 
                 0.9642 
               
               
                 CM1 - longer 
                 20.5 
                 2.9739 
                 2.35 ± 0.02E−8 
                 1.08 ± 0.01E5 
                 2.54 ± 0.01E−3 
                 0.9939 
               
               
                 association 
                 5.11 
                 2.738 
                 2.35 ± 0.02E−8 
                 1.08 ± 0.01E5 
                 2.54 ± 0.01E−3 
                 0.9939 
               
               
                   
                 1.02 
                 2.5002 
                 2.35 ± 0.02E−8 
                 1.08 ± 0.01E5 
                 2.54 ± 0.01E−3 
                 0.9939 
               
               
                   
                 0.2045 
                 2.0092 
                 2.35 ± 0.02E−8 
                 1.08 ± 0.01E5 
                 2.54 ± 0.01E−3 
                 0.9939 
               
               
                   
                 0.1022 
                 1.6825 
                 2.35 ± 0.02E−8 
                 1.08 ± 0.01E5 
                 2.54 ± 0.01E−3 
                 0.9939 
               
               
                   
                 0.0511 
                 1.3298 
                 2.35 ± 0.02E−8 
                 1.08 ± 0.01E5 
                 2.54 ± 0.01E−3 
                 0.9939 
               
               
                   
                 0.0102 
                 0.4913 
                 2.35 ± 0.02E−8 
                 1.08 ± 0.01E5 
                 2.54 ± 0.01E−3 
                 0.9939 
               
               
                 CM7 - longer 
                 20.5 
                 2.4923 
                 2.97 ± 0.05E−7 
                 2.15 ± 0.04E4 
                 6.38 ± 0.04E−3 
                 0.99 
               
               
                 association 
                 5.11 
                 2.0067 
                 2.97 ± 0.05E−7 
                 2.15 ± 0.04E4 
                 6.38 ± 0.04E−3 
                 0.99 
               
               
                   
                 1.02 
                 1.5108 
                 2.97 ± 0.05E−7 
                 2.15 ± 0.04E4 
                 6.38 ± 0.04E−3 
                 0.99 
               
               
                   
                 0.2045 
                 0.8611 
                 2.97 ± 0.05E−7 
                 2.15 ± 0.04E4 
                 6.38 ± 0.04E−3 
                 0.99 
               
               
                   
                 0.1022 
                 0.5715 
                 2.97 ± 0.05E−7 
                 2.15 ± 0.04E4 
                 6.38 ± 0.04E−3 
                 0.99 
               
               
                   
                 0.0511 
                 0.3578 
                 2.97 ± 0.05E−7 
                 2.15 ± 0.04E4 
                 6.38 ± 0.04E−3 
                 0.99 
               
               
                   
                 0.0102 
                 0.099 
                 2.97 ± 0.05E−7 
                 2.15 ± 0.04E4 
                 6.38 ± 0.04E−3 
                 0.99 
               
               
                 i53 - matched 
                 20.5 
                 1.954 
                 3.87 ± 0.14E−6 
                 2.30 ± 0.08E4 
                 8.92 ± 0.13E−2 
                 0.9956 
               
               
                 dosage range 
                 5.11 
                 1.2658 
                 3.87 ± 0.14E−6 
                 2.30 ± 0.08E4 
                 8.92 ± 0.13E−2 
                 0.9956 
               
               
                 for longer 
                 1.02 
                 0.6247 
                 3.87 ± 0.14E−6 
                 2.30 ± 0.08E4 
                 8.92 ± 0.13E−2 
                 0.9956 
               
               
                 association 
                 0.2045 
                 0.1877 
                 3.87 ± 0.14E−6 
                 2.30 ± 0.08E4 
                 8.92 ± 0.13E−2 
                 0.9956 
               
               
                 experiment 
                 0.1022 
                 0.104 
                 3.87 ± 0.14E−6 
                 2.30 ± 0.08E4 
                 8.92 ± 0.13E−2 
                 0.9956 
               
               
                   
                 0.0511 
                 0.0537 
                 3.87 ± 0.14E−6 
                 2.30 ± 0.08E4 
                 8.92 ± 0.13E−2 
                 0.9956 
               
               
                   
                 0.0102 
                 0.0176 
                 3.87 ± 0.14E−6 
                 2.30 ± 0.08E4 
                 8.92 ± 0.13E−2 
                 0.9956 
               
               
                   
               
            
           
         
       
     
     Example 3. Ubvs with Higher Affinity for 53BP1 than i53 are More Effective at Improving Rates of HDR 
     In order to test the effects of the improved affinity of the combination mutant Ubvs for 53BP1 on HIDR, i53, CM1, and CM7 Ubvs were purified and used for testing in human cells (Table 3). The Ubvs were delivered alongside Cas9 V3 (JDT) RNP targeting a site in SERPINC1 with single stranded Alt-R HIDR Donor Oligoes (JDR) to introduce an EcoR1 cut site sequence (GAATTC) at the Cas9 cut site upon successful HIDR (Table 5, see methods described below). A range of Ubvs doses was tested from 12.5 to 200 μM. The improved affinity ubiquitin variants required ˜10 fold lower dose for maximum effectiveness and the HDR rates were improved beyond what could be achieved with the i53 peptide ( FIG.  6   ). 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Guide and donor information 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                 Proto- 
                   
                   
                   
               
               
                   
                   
                 spacer 
                   
                   
                 ssODN   
               
               
                   
                 coor- 
                 [SEQ 
                   
                 Guide  
                 sequence 
               
               
                   
                 di- 
                 ID 
                 coor- 
                 sequence 
                 [SEQ ID 
               
               
                   
                 nates 
                 NO: 
                 di- 
                 [SEQ ID 
                 NO: 
               
               
                 Gene 
                 (hg38) 
                 1101] 
                 nates 
                 NO: 1102] 
                 1103] 
               
               
                   
               
               
                 SERPINCI 
                 chr1: 
                 ACCTCTG 
                 chr1: 
                 mA*mC*mC*r 
                 /Alt-R- 
               
               
                   
                 173, 
                 GAAAAAG 
                 173, 
                 UrCrUrGrGr 
                 HDR1/A* 
               
               
                   
                 903, 
                 GTAAGA 
                 917, 
                 ArArArArAr 
                 T*TCCAA 
               
               
                   
                 800- 
                   
                 213- 
                 GrGrUrArAr 
                 TGTGATA 
               
               
                   
                 173, 
                   
                 173, 
                 GrArGrUrUr 
                 GGAACTG 
               
               
                   
                 917, 
                   
                 917, 
                 UrUrArGrAr 
                 TAACCTC 
               
               
                   
                 327 
                   
                 232 
                 GrCrUrArGr 
                 TGGAAAA 
               
               
                   
                   
                   
                   
                 ArArArUrAr 
                 AGGTAGA 
               
               
                   
                   
                   
                   
                 GrCrArArGr 
                 ATTCAGA 
               
               
                   
                   
                   
                   
                 UrUrArArAr 
                 GGGGTGA 
               
               
                   
                   
                   
                   
                 ArUrArArGr 
                 GCTTTCC 
               
               
                   
                   
                   
                   
                 GrCrUrArGr 
                 CCTTGCC 
               
               
                   
                   
                   
                   
                 UrCrCrGrUr 
                 TGCCCCT 
               
               
                   
                   
                   
                   
                 UrArUrCrAr 
                 ACTGGGT 
               
               
                   
                   
                   
                   
                 ArCrUrUrGr 
                 *T*T/ 
               
               
                   
                   
                   
                   
                 ArArArArAr 
                 Alt-R- 
               
               
                   
                   
                   
                   
                 GrUrGrGrCr 
                 HDR2/ 
               
               
                   
                   
                   
                   
                 ArCrCrGrAr 
                   
               
               
                   
                   
                   
                   
                 GrUrCrGrGr 
                   
               
               
                   
                   
                   
                   
                 UrGrCmU*mU 
                   
               
               
                   
                   
                   
                   
                 *mU*rU 
               
               
                   
               
            
           
         
       
     
     Genome editing was mediated via IDT Alt-R Cas9 ribonucleoprotein (RNP) complexes delivered by Lonza nucleofection in concert with single-stranded oligodeoxynucleotide (ssODN) HDR repair templates. The specific repair event was the insertion of the 6-nt EcoR1 sequence (5′-GAATTC-3′) directly at the canonical Sp Cas9 cut site (between bases 3 and 4 in the 5′-direction from the PAM sequence). HDR complexes were formed with a nuclease-specific guide for the SERPINC1 gene (Table 5). HDR template consisted of a chemically modified ssODN synthesized as IDT Alt-R HDR Donor Oligos with the Alt-R modification. The sequence contains 40-nt homology arms (HA) on the 5′-end, the 6-nt EcoR1 sequence in the center of the oligo and 40-nt HA on the 3′-end (Table 5). The 86-nt repair template was homologous to the non-targeting strand of dsDNA, where targeting/non-targeting is defined with respect to the guide RNA sequence and the presence of the PAM sequence identifying the targeting strand. The RNPs were generated by complexing IDT Alt-R Cas9 to IDT Alt-R sgRNA at a 1:1.2 ratio of protein to guide to give a final concentration of 2 uM Cas9 with 2.4 uM guide RNA where final concentration refers to the concentration in the final cells, protein, RNA, and DNA mix. The Ubv protein was added to the Cas9 RNP at varying amounts (200 μM down to 12.5 μM final concentration) along with donor DNA at a final concentration of 2 uM. Cas9 RNP, donor, and Ubv protein was delivered into HEK293 cells using the Lonza 96-well Shuttle and nucleofection protocol 96-DS-150. The cells were allowed to grow for 48 hours, after which genomic DNA was isolated using QuickExtract (Epicentre). HDR was measured by NGS. 
     Example 4. Additional Stacking of Screen-Identified Mutations Resulted in the Generation of Ubiquitin Variants with Improved In Vitro Affinity for 53BP1 Relative to i53 that do not Contain any of the Original i53 Mutations 
     Testing of additional combinations of mutations identified variants with improved affinity over the previous best variant, CM1. In order to further validate the amino acids changes identified in the two-hybrid screen as candidates for improving the affinity of our Ubvs for 53BP1, a subset of the top hits from the screen were individually added to i53, the results of this screen are shown in  FIG.  7   . For graphs in this invention disclosure labeled as “Fold change in affinity”, affinity is graphed as the association constant (KA) of the ubiquitin variant being tested divided by the KA of the reference ubiquitin variant, typically the base construct upon which further mutations are stacked as determined by calculating each affinity for binding a fragment of 53BP1 (Table 6) using biolayer interferometry (BLI). The BLI steady-state response versus 53BP1 fragment concentration was plotted in prism to calculate the Kd using a one site-specific binding nonlinear fit model. If the affinity of a ubiquitin variant being tested is higher (binding is tighter) than for the reference ubiquitin variant, then the fold change in affinity will be &gt;1. Of the mutations tested, the majority were shown to result in improved affinity (fold change &gt;1) relative to i53, indicating that positive hits from two-hybrid screen reliably identified mutations that improved affinity. In order to validate if CM1 was the best starting combination of mutations for additional stacking, the contribution of each of the 9 mutations present in CM1 relative to i53 was analyzed and is shown in  FIG.  8   . Loss of any of the mutations resulted in reduced affinity indicating that each mutation contributes to the overall affinity of CM1 for binding 53BP1. Additional mutations were then added to CM1 either alone or in combination to determine if the affinity could be further improved. 
     The results of that experiment are shown in  FIG.  9   . Many individual and combinations of mutations were identified that improve the affinity of CM1 for 53BP1 ( FIGS.  9 A and  9 B ) with the best individual mutations improving affinity by approximately 25%. Subsequent combining of the groups of mutations or parts of the groups of mutations identified as beneficial resulted in ubiquitin variants with a further benefit to affinity ( FIG.  9 B ), with maximal benefit being an approximately 50% improvement in affinity over CM1. Subsequent additional stacking identified combinations of mutations that provided a 2-3 fold benefit to affinity over CM1 ( FIG.  9 C ). Notably, the combinations of (M1Y, V26I, L73M-CM131), (E18M, K48T, E51D, S57G-CM134), (E16M, N25V, Q40E, S49L-CM135), (R74Q-comparison of CM136 to CM137 and CM140 to CM141), and (A44T, S49L-CM139) were notably beneficial when added to a base of CM113. All of the combinations tested had improved affinity over CM1. 
     To narrow down which variant may have the best activity in cells CM138, CM142, CM143, CM147, CM149, CM158 were selected for additional testing. The 53BP1-binding deficiency mutant amino acid substitutions (P69L and L70V) were added to CM142, CM143, CM147, CM149, and CM158 and the effect on affinity was measured using BLI 11 . The results are shown in  FIG.  10   , with CM142 having the best tolerance for the DM mutations. CM142 and CM142-DM (CM203) were also tested for their ability to improve the rate of HDR in cells ( FIG.  10 B ). CM142 was found to provide a significantly increased benefit to HDR over i53. Further, CM142-DM, despite having the mutations that eliminate i53 binding to 53BP1, also showed an improved benefit to HDR over i53. 
     Screening of possible alternative mutations at positions mutated in i53 resulted in the identification of high affinity ubiquitin variants that do not include any of the mutations present in i53. Given the tolerance of CM142 for the DM mutations ( FIG.  10 A ), additional screening was performed at positions 62, 69, and 70 to identify alternative beneficial amino acids at those positions. A screen was conducted using CM142-DM (CM203) as the base construct and positions 69 or 70 were individually mutated to the 18 amino acids not present in i53 or wildtype ubiquitin. The results are shown in  FIG.  11 A . For position 69, 69A and 69G were most beneficial. For position 70, 70M, 70F and 70C were most beneficial. The only i53 mutations remaining in CM142 DM are Q2L, Q62L, E64D, and T66K relative to wild-type ubiquitin ( FIG.  11 E ). From our two-hybrid screen L2M, L62P, D64S, and K66E were identified as providing the second-best benefit to affinity relative to the published mutations in i53 at those positions (data not shown). L2M, L62P, D64S, and K66E mutations were added to CM142 DM and this variant (CM476- FIG.  11 E ) was used as a baseline construct for testing combinations of DM position mutations. Further, CM476+L69A (CM429) was used to screen all possible alternatives at position 62 since Q62P was a poor alternative to Q62L (relative to wildtype ubiquitin) based on the two-hybrid screen. The result of this screening is shown in  FIGS.  11 B and  11 C . Relative to CM142 DM, L69A+V70M was identified as the most beneficial combination of mutations at positions 69 and 70, and A, C, T, and V were identified as the most beneficial amino acids at position 62. Together, these data indicate that some combination of CM142 DM plus L69A+V70M and either P62A, P62C, P62T, or P62V (CM465, CM467, CM468, and CM469 in Table 6) relative to CM476 will result in a variant containing no i53 mutations with the best affinity for 53BP1. The V70M mutation was found to affect purification (data not shown), so CM455 (containing the P62T and L69A mutations relative to CM476,  FIG.  11 E ) was selected for further testing. The affinity CM455, CM1, and i53 for binding a fragment of 53BP1 as measured by BLI is shown in  FIG.  11 D . The affinity of CM455 for binding 53BP1 is on par with or slightly better than that of CM1, despite having none of the amino acid changes present in i53 relative to wildtype ubiquitin other than removal of the terminal glycine residues. 
     To determine if CM455 is able to enhance rates of HDR, we tested its ability to improve rates of HDR measured by introduction of an EcoR1 cut site sequence at SERPINC1 as described in Example 3 with the exception that editing was measured using next generation sequencing. The results are shown in  FIG.  11 F . CM455 was able to boost HDR rates to higher levels and at lower concentrations than i53. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Amino acid and DNA sequences described in Example 4 
               
            
           
           
               
               
               
               
            
               
                   
                 Amino acid 
                   
                   
               
               
                 Name 
                 changes 
                   
                   
               
               
                 [SEQ ID 
                 relative  
                 Protein 
                   
               
               
                 NOS] a   
                 to i53 
                 Sequence 
                 DNA sequence 
               
               
                   
               
               
                 i53 
                 None 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [3; 883] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 i53 DM 
                 P69L, L70V 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGGGGGTCGG 
               
               
                 [234; 884] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHLVLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCTGGTTCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 i53 K6R 
                 K6R 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGGGGGTCGG 
               
               
                 [235; 885] 
                   
                 GMLIFVRTLTGKT 
                 GCATGTTGATTTTCGTACGCACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 i53 T14E 
                 T14E 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGGGGGTCGG 
               
               
                 [236; 886] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 IELEVEPSDTIENV 
                 AAGACTATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 i53 K33A 
                 K33A 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGGGGGTCGG 
               
               
                 [237; 887] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDAEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATGCCGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 i53 A46Q 
                 A46Q 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGGGGGTCGG 
               
               
                 [238; 888] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFQGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 i53 K63I 
                 K63I 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGGGGGTCGG 
               
               
                 [239; 889] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILID 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 ATTGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 i53 S65P 
                 S65P 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGGGGGTCGG 
               
               
                 [240; 890] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 PKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 MBP 
                 N/A 
                 MKIEEGKLVIWIN 
                 ATGAAAATCGAAGAAGGTAAACTGGTAATCT 
               
               
                 tagged 
                   
                 GDKGYNGLAEV 
                 GGATTAACGGCGATAAAGGCTATAACGGTCT 
               
               
                 53BP1 
                   
                 GKKFEKDTGIKVT 
                 CGCTGAAGTCGGTAAGAAATTCGAGAAAGAT 
               
               
                 fragment 
                   
                 VEHPDKLEEKFP 
                 ACCGGAATTAAAGTCACCGTTGAGCATCCGG 
               
               
                 [245; 891] 
                   
                 QVAATGDGPDII 
                 ATAAACTGGAAGAGAAATTCCCACAGGTTGC 
               
               
                   
                   
                 FWAHDRFGGYA 
                 GGCAACTGGCGATGGCCCTGACATTATCTTCT 
               
               
                   
                   
                 QSGLLAEITPDKA 
                 GGGCACACGACCGCTTTGGTGGCTACGCTCA 
               
               
                   
                   
                 FQDKLYPFTWDA 
                 ATCTGGCCTGTTGGCTGAAATCACCCCGGAC 
               
               
                   
                   
                 VRYNGKLIAYPIA 
                 AAAGCGTTCCAGGACAAGCTGTATCCGTTTAC 
               
               
                   
                   
                 VEALSLIYNKDLL 
                 CTGGGATGCCGTACGTTACAACGGCAAGCTG 
               
               
                   
                   
                 PNPPKTWEEIPA 
                 ATTGCTTACCCGATCGCTGTTGAAGCGTTATC 
               
               
                   
                   
                 LDKELKAKGKSAL 
                 GCTGATTTATAACAAAGATCTGCTGCCGAACC 
               
               
                   
                   
                 MFNLQEPYFTW 
                 CGCCAAAAACCTGGGAAGAGATCCCGGCGCT 
               
               
                   
                   
                 PLIAADGGYAFKY 
                 GGATAAAGAACTGAAAGCGAAAGGTAAGAG 
               
               
                   
                   
                 ENGKYDIKDVGV 
                 CGCGCTGATGTTCAACCTGCAAGAACCGTACT 
               
               
                   
                   
                 DNAGAKAGLTFL 
                 TCACCTGGCCGCTGATTGCTGCTGACGGGGG 
               
               
                   
                   
                 VDLIKNKHMNA 
                 TTATGCGTTCAAGTATGAAAACGGCAAGTAC 
               
               
                   
                   
                 DTDYSIAEAAFNK 
                 GACATTAAAGACGTGGGCGTGGATAACGCTG 
               
               
                   
                   
                 GETAMTINGPW 
                 GCGCGAAAGCGGGTCTGACCTTCCTGGTTGA 
               
               
                   
                   
                 AWSNIDTSKVNY 
                 CCTGATTAAAAACAAACACATGAATGCAGAC 
               
               
                   
                   
                 GVTVLPTFKGQP 
                 ACCGATTACTCCATCGCAGAAGCTGCCTTTAA 
               
               
                   
                   
                 SKPFVGVLSAGIN 
                 TAAAGGCGAAACAGCGATGACCATCAACGGC 
               
               
                   
                   
                 AASPNKELAKEFL 
                 CCGTGGGCATGGTCCAACATCGACACCAGCA 
               
               
                   
                   
                 ENYLLTDEGLEAV 
                 AAGTGAATTATGGTGTAACGGTACTGCCGAC 
               
               
                   
                   
                 NKDKPLGAVALK 
                 CTTCAAGGGTCAACCATCCAAACCGTTCGTTG 
               
               
                   
                   
                 SYEEELAKDPRIA 
                 GCGTGCTGAGCGCAGGTATTAACGCCGCCAG 
               
               
                   
                   
                 ATMENAQKGEI 
                 TCCGAACAAAGAGCTGGCAAAAGAGTTCCTC 
               
               
                   
                   
                 MPNIPQMSAFW 
                 GAAAACTATCTGCTGACTGATGAAGGTCTGG 
               
               
                   
                   
                 YAVRTAVINAAS 
                 AAGCGGTTAATAAAGACAAACCGCTGGGTGC 
               
               
                   
                   
                 GRQTVDEALKDA 
                 CGTAGCGCTGAAGTCTTACGAGGAAGAGTTG 
               
               
                   
                   
                 QTNSSSNNNNN 
                 GCGAAAGATCCACGTATTGCCGCCACTATGG 
               
               
                   
                   
                 NNNNNLGIEENL 
                 AAAACGCCCAGAAAGGTGAAATCATGCCGAA 
               
               
                   
                   
                 YFQGHMNSFVG 
                 CATCCCGCAGATGTCCGCTTTCTGGTATGCCG 
               
               
                   
                   
                 LRVVAKWSSNGY 
                 TGCGTACTGCGGTGATCAACGCCGCCAGCGG 
               
               
                   
                   
                 FYSGKITRDVGA 
                 TCGTCAGACTGTCGATGAAGCCCTGAAAGAC 
               
               
                   
                   
                 GKYKLLFDDGYE 
                 GCGCAGACTAATTCGAGCTCGAACAACAACA 
               
               
                   
                   
                 CDVLGKDILLCDP 
                 ACAATAACAATAACAACAACCTCGGGATCGA 
               
               
                   
                   
                 IPLDTEVTALSED 
                 GGAAAATCTGTATTTTCAGGGCCACATGAAT 
               
               
                   
                   
                 EYFSAGVVKGHR 
                 AGCTTTGTTGGTCTGCGTGTTGTTGCAAAATG 
               
               
                   
                   
                 KESGELYYSIEKE 
                 GTCAAGCAATGGTTATTTCTACAGCGGCAAA 
               
               
                   
                   
                 GQRKWYKRMA 
                 ATCACCCGTGATGTTGGTGCAGGTAAATACA 
               
               
                   
                   
                 VILSLEQGNRLRE 
                 AACTGCTGTTTGATGATGGTTATGAATGTGAT 
               
               
                   
                   
                 QYGLG 
                 GTGCTGGGCAAAGATATTCTGCTGTGTGATC 
               
               
                   
                   
                   
                 CGATTCCGCTGGATACCGAAGTTACCGCACT 
               
               
                   
                   
                   
                 GAGCGAAGATGAATATTTCAGTGCCGGTGTT 
               
               
                   
                   
                   
                 GTTAAAGGCCATCGTAAAGAAAGCGGTGAAC 
               
               
                   
                   
                   
                 TGTATTACAGCATTGAAAAAGAAGGTCAGCG 
               
               
                   
                   
                   
                 CAAATGGTATAAACGTATGGCAGTTATTCTGA 
               
               
                   
                   
                   
                 GCCTGGAACAGGGTAATCGTCTGCGTGAACA 
               
               
                   
                   
                   
                 GTATGGTCTGGGT 
               
               
                   
               
               
                 SM1 
                 M1H 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [246; 892] 
                   
                 GHLIFVKTLTGKTI 
                 GCCATTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 TLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM2 
                 K6R 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [247; 893] 
                   
                 GMLIFVRTLTGKT 
                 GCATGTTGATTTTCGTACGCACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM3 
                 T7M 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [248; 894] 
                   
                 GMLIFVKMLTGK 
                 GCATGTTGATTTTCGTAAAGATGTTGACTGGA 
               
               
                   
                   
                 TITLEVEPSDTIEN 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 VKAKIQDKEGIPP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM4 
                 T12M 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [249; 895] 
                   
                 GMLIFVKTLTGK 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 MITLEVEPSDTIE 
                 AAGATGATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 NVKAKIQDKEGIP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PDQQRLAFAGKS 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 LEDGRTLSDYNIL 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 KDSKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM5 
                 T14E 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [250; 896] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 IELEVEPSDTIENV 
                 AAGACTATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM6 
                 E16M 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [251; 897] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLMVEPSDTIEN 
                 AAGACTATCACTTTGATGGTGGAGCCTTCCGA 
               
               
                   
                   
                 VKAKIQDKEGIPP 
                 TACTATCGAGAATGTTAAGGCCAAAATCCAA 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 GATAAGGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 GCCTTGCTTTTGCCGGGAAGAGCCTGGAGGA 
               
               
                   
                   
                 DSKLHPLLRLR 
                 CGGTCGCACACTGTCTGACTATAACATTCTTA 
               
               
                   
                   
                   
                 AAGATTCTAAATTGCATCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 SM7 
                 E18M 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [252; 898] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVMPSDTIEN 
                 AAGACTATCACTTTGGAAGTGATGCCTTCCGA 
               
               
                   
                   
                 VKAKIQDKEGIPP 
                 TACTATCGAGAATGTTAAGGCCAAAATCCAA 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 GATAAGGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 GCCTTGCTTTTGCCGGGAAGAGCCTGGAGGA 
               
               
                   
                   
                 DSKLHPLLRLR 
                 CGGTCGCACACTGTCTGACTATAACATTCTTA 
               
               
                   
                   
                   
                 AAGATTCTAAATTGCATCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 SM8 
                 N25V 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [253; 899] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIEVV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM9 
                 V26I 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [254; 900] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENI 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 GATAAGGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 GCCTTGCTTTTGCCGGGAAGAGCCTGGAGGA 
               
               
                   
                   
                 SKLHPLLRLR 
                 CGGTCGCACACTGTCTGACTATAACATTCTTA 
               
               
                   
                   
                   
                 AAGATTCTAAATTGCATCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 SM10 
                 Q31W 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [255; 901] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIWDKEGIPP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCTG 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 GGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM11 
                 Q31C 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [256; 902] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKICDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCTG 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 CGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM12 
                 Q31F 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [257; 903] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIFDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCTTC 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 GATAAGGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 GCCTTGCTTTTGCCGGGAAGAGCCTGGAGGA 
               
               
                   
                   
                 SKLHPLLRLR 
                 CGGTCGCACACTGTCTGACTATAACATTCTTA 
               
               
                   
                   
                   
                 AAGATTCTAAATTGCATCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 SM13 
                 K33S 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [258; 904] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDSEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATTCTGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM14 
                 K33H 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [259; 905] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDHEGIPP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM15 
                 K33A 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [260; 906] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDAEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATGCCGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM16 
                 P38L 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [261; 907] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPLD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTTTGGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM17 
                 R42W 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [262; 908] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQWLAFAGKSL 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 TGGCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM18 
                 A44T 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [263; 909] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLTFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTACTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM19 
                 A46Q 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [264; 910] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFQGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM20 
                 K48T 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [265; 911] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGTSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGACTAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM21 
                 R54Y 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [266; 912] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGYTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTTATACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM22 
                 S57G 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [267; 913] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLGDYNILK 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 ACGGTCGCACACTGGGGGACTATAACATTCT 
               
               
                   
                   
                   
                 TAAAGATTCTAAATTGCATCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 SM23 
                 K63I 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [268; 914] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILID 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 ATTGATTCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 SM24 
                 S65P 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [269; 915] 
                   
                 GMLIFVKTLTGKT 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 PKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM1 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [241; 916] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM7 
                 K6R, K33H, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [242; 917] 
                 A46Q, S65P 
                 GMLIFVRTLTGKT 
                 GCATGTTGATTTTCGTACGCACGTTGACTGGA 
               
               
                   
                   
                 ITLEVEPSDTIENV 
                 AAGACTATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 KAKIQDHEGIPP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 DQQRLAFQGKSL 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 DPKLHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAATTGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM13 
                 T7M, T14E, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [243; 918] 
                 A46Q, L67K 
                 GMLIFVKMLTGK 
                 GCATGTTGATTTTCGTAAAGATGTTGACTGGA 
               
               
                   
                   
                 TIELEVEPSDTIEN 
                 AAGACTATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 VKAKIQDKEGIPP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 DQQRLAFQGKSL 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 DSKKHPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATTCTAAAAAGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM26 
                 T12M, K33H, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [244; 919] 
                 A46Q, H68M 
                 GMLIFVKTLTGK 
                 GCATGTTGATTTTCGTAAAGACGTTGACTGGA 
               
               
                   
                   
                 MITLEVEPSDTIE 
                 AAGATGATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                   
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDSKLMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATTCTAAATTGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM44 
                 T7M, T12M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [270; 920] 
                 T14E, K33H, 
                 GMLIFVKMLTGK 
                 GCATGTTGATTTTCGTAAAGATGTTGACTGGA 
               
               
                   
                 A46Q, S65P, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 L67K, H68M 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM45 
                 K6R, T12M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [271; 921] 
                 T14E, K33H, 
                 GMLIFVRTLTGK 
                 GCATGTTGATTTTCGTACGCACGTTGACTGGA 
               
               
                   
                 A46Q, S65P, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 L67K, H68M 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM46 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [272; 922] 
                 T14E, K33H, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 A46Q, S65P, 
                 TIELEVEPSDTIEN 
                 AAGACTATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 L67K, H68M 
                 VKAKIQDHEGIPP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 DQQRLAFQGKSL 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 DPKKMPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM47 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [273; 923] 
                 T12M, K33H, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 A46Q, S65P, 
                 MITLEVEPSDTIE 
                 AAGATGATCACTTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 L67K, H68M 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM48 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [274; 924] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 A46Q, S65P, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 L67K, H68M 
                 NVKAKIQDKEGIP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PDQQRLAFQGKS 
                 AGATAAGGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 LEDGRTLSDYNIL 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM49 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [275; 925] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, S65P, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 L67K, H68M 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PPDQQRLAFAGK 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 SLEDGRTLSDYNI 
                 CGCCTTGCTTTTGCCGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM50 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [276; 926] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 L67K, H68M 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDSKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATTCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM51 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [277; 927] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, H68M 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKLMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAATTGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM52 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [278; 928] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                   
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKHPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGCATCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM62 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [279; 929] 
                 T12M, T14E, 
                 GHLIFVRMLTGK 
                 GCCATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, M1H 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM63 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [280; 930] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, M1Y 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM64 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [281; 931] 
                 T12M, T14H, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIHLEVEPSDTIE 
                 AAGATGATCCATTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM65 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [282; 932] 
                 T12M, T14D, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIDLEVEPSDTIE 
                 AAGATGATCGATTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM66 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [283; 933] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELMVEPSDTIE 
                 AAGATGATCGAGTTGATGGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E16M 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM67 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [284; 934] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELTVEPSDTIE 
                 AAGATGATCGAGTTGACTGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E16T 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM68 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [285; 935] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E18M 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM69 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [286; 936] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVYPSDTIE 
                 AAGATGATCGAGTTGGAAGTGTATCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E18Y 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM70 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [287; 937] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVLPSDTIE 
                 AAGATGATCGAGTTGGAAGTGTTGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E18L 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM71 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [288; 938] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVFPSDTIE 
                 AAGATGATCGAGTTGGAAGTGTTCCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E18F 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM72 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [289; 939] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, N25V 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM73 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [290; 940] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 EVKAKIQDHEGIP 
                 ATACTATCGAGGAGGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, N25E 
                 PDQQRLAFQGKS 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 LEDGRTLSDYNIL 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM74 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [291; 941] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, V26I 
                 PDQQRLAFQGKS 
                 GATCATGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                   
                 LEDGRTLSDYNIL 
                 GCCTTGCTTTTCAAGGGAAGAGCCTGGAGGA 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 CGGTCGCACACTGTCTGACTATAACATTCTTA 
               
               
                   
                   
                   
                 AAGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM75 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [292; 942] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKICDHEGIP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCTG 
               
               
                   
                 H68M, Q31C 
                 PDQQRLAFQGKS 
                 CGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 LEDGRTLSDYNIL 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM76 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [293; 943] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIWDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCTG 
               
               
                   
                 H68M, Q31W 
                 PPDQQRLAFQG 
                 GGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM77 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [294; 944] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIFDHEGIP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCTTC 
               
               
                   
                 H68M, Q31F 
                 PDQQRLAFQGKS 
                 GATCATGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                   
                 LEDGRTLSDYNIL 
                 GCCTTGCTTTTCAAGGGAAGAGCCTGGAGGA 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 CGGTCGCACACTGTCTGACTATAACATTCTTA 
               
               
                   
                   
                   
                 AAGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM78 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [295; 945] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQAHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, D32A 
                 PPDQQRLAFQG 
                 AGCCCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM79 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [296; 946] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33S, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDSEGIP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M 
                 PDQQRLAFQGKS 
                 AGATTCTGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 LEDGRTLSDYNIL 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM80 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [297; 947] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33Q, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDQEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M 
                 PPDQQRLAFQG 
                 AGATCAAGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM81 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [298; 948] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33A, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDAEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M 
                 PPDQQRLAFQG 
                 AGATGCCGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM82 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [299; 949] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, P38L 
                 PLDQQRLAFQGK 
                 AGATCATGAAGGGATTCCTTTGGATCAACAA 
               
               
                   
                   
                 SLEDGRTLSDYNI 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM83 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [300; 950] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, P38C 
                 PCDQQRLAFQG 
                 AGATCATGAAGGGATTCCTTGCGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM84 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [301; 951] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, Q40E 
                 PPDEQRLAFQGK 
                 AGATCATGAAGGGATTCCTCCAGATGAGCAA 
               
               
                   
                   
                 SLEDGRTLSDYNI 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM87 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [302; 952] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, R42H 
                 PPDQQHLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CATCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM88 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [303; 953] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, R42F 
                 PPDQQFLAFQGK 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 SLEDGRTLSDYNI 
                 TTCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM89 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [304; 954] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, A44T 
                 PPDQQRLTFQGK 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 SLEDGRTLSDYNI 
                 CGCCTTACTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM90 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [305; 955] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, K48T 
                 PPDQQRLAFQGT 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 SLEDGRTLSDYNI 
                 CGCCTTGCTTTTCAAGGGACTAGCCTGGAGG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM92 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [306; 956] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, S49L 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KLLEDGRTLSDYN 
                 CGCCTTGCTTTTCAAGGGAAGTTGCTGGAGG 
               
               
                   
                   
                 ILKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM93 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [307; 957] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, S49M 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KMLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGATGCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM94 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [308; 958] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E51D 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLDDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGATG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM95 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [309; 959] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, R54Y 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGYTLSDYN 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 ILKDPKKMPLLRL 
                 ACGGTTATACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM98 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [310; 960] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, S57G 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLGDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGGGGGACTATAACATTCT 
               
               
                   
                   
                 LR 
                 TAAAGATCCTAAAAAGATGCCACTGCTGCGC 
               
               
                   
                   
                   
                 TTGCGT 
               
               
                   
               
               
                 CM101 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [311; 961] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NLLKDPKKMPLL 
                 ACGGTCGCACACTGTCTGACTATAACTTGCTT 
               
               
                   
                   
                 RLR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM102 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [312; 962] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, K63I 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILIDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LR 
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM103 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [313; 963] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65H, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDHKKMPLL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 RLR 
                 AAAGATCATAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM104 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [314; 964] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, L73M 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 MR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCA 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM105 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [315; 965] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, R74Q 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LQ 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCAA 
               
               
                   
               
               
                 CM107 
                 T7M, T12M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [316; 966] 
                 T14E, K33H, 
                 GMLIFVKMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 A46Q, S65P, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 L67K, H68M, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 P69L, L70V 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMLVL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 RLR 
                 AAAGATCCTAAAAAGATGTTGGTACTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM108 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [317; 967] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVYPSDTIE 
                 AAGATGATCGAGTTGGAAGTGTATCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E18Y, 
                 PLDQQRLAFQGK 
                 AGATCATGAAGGGATTCCTTTGGATCAACAA 
               
               
                   
                 P38L, S49L, 
                 LLEDGRTLGDYNI 
                 CGCCTTGCTTTTCAAGGGAAGTTGCTGGAGG 
               
               
                   
                 S57G 
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGGGGGACTATAACATTCT 
               
               
                   
                   
                 R 
                 TAAAGATCCTAAAAAGATGCCACTGCTGCGC 
               
               
                   
                   
                   
                 TTGCGT 
               
               
                   
               
               
                 CM110 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [318; 968] 
                 T12M, T14E, 
                 GHLIFVRMLTGK 
                 GCCATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, M1H, 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 R74Q 
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 LQ 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCAA 
               
               
                   
               
               
                 CM111 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [319; 969] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, M1Y, 
                 PDQQRLAFQGKS 
                 GATCATGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                 V26I, L73M 
                 LEDGRTLSDYNIL 
                 GCCTTGCTTTTCAAGGGAAGAGCCTGGAGGA 
               
               
                   
                   
                 KDPKKMPLLRM 
                 CGGTCGCACACTGTCTGACTATAACATTCTTA 
               
               
                   
                   
                 R 
                 AAGATCCTAAAAAGATGCCACTGCTGCGCAT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM112 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [320; 970] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, N25V, 
                 PPDEQRLAFQGK 
                 AGATCATGAAGGGATTCCTCCAGATGAGCAA 
               
               
                   
                 Q40E, E51D 
                 SLDDGRTLSDYNI 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGATG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM113 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [321; 971] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PPDQQRLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 K63I 
                 KSLEDGRTLSDY 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 NLLIDPKKMPLLR 
                 ACGGTCGCACACTGTCTGACTATAACTTGCTT 
               
               
                   
                   
                 LR 
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM114 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [322; 972] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E18M, 
                 PPDQQRLAFQGT 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 K48T, E51D, 
                 SLDDGRTLGDYN 
                 CGCCTTGCTTTTCAAGGGACTAGCCTGGATGA 
               
               
                   
                 S57G 
                 ILKDPKKMPLLRL 
                 CGGTCGCACACTGGGGGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM115 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [323; 973] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELMVEPSDTIE 
                 AAGATGATCGAGTTGATGGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E16M, 
                 PPDEQRLAFQGK 
                 AGATCATGAAGGGATTCCTCCAGATGAGCAA 
               
               
                   
                 N25V, Q40E, 
                 LLEDGRTLSDYNI 
                 CGCCTTGCTTTTCAAGGGAAGTTGCTGGAGG 
               
               
                   
                 S49L 
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACATTCTT 
               
               
                   
                   
                 R 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM116 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [324; 974] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, V26I, 
                 PDQYRLAFQGKL 
                 GATCATGAAGGGATTCCTCCAGATCAATATC 
               
               
                   
                 Q41Y, S49L, 
                 LEDGRTLGDYNIL 
                 GCCTTGCTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S57G 
                 KDPKKMPLLRLR 
                 CGGTCGCACACTGGGGGACTATAACATTCTT 
               
               
                   
                   
                   
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM117 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [325; 975] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKICDHEGIP 
                 ATACTATCGAGAATGTTAAGGCCAAAATCTG 
               
               
                   
                 H68M, Q31C, 
                 PDQQHLAFQGK 
                 CGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 R42H, S57G 
                 SLEDGRTLGDYNI 
                 CATCTTGCTTTTCAAGGGAAGAGCCTGGAGG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 ACGGTCGCACACTGGGGGACTATAACATTCT 
               
               
                   
                   
                 R 
                 TAAAGATCCTAAAAAGATGCCACTGCTGCGC 
               
               
                   
                   
                   
                 TTGCGT 
               
               
                   
               
               
                 CM118 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [326; 976] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 WVVAKIFDHEGIP 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCTTC 
               
               
                   
                 H68M, E18M, 
                 PDQQHLAFQGT 
                 GATCATGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                 N25V, Q31F, 
                 SLEDGYTLGDYNI 
                 ATCTTGCTTTTCAAGGGACTAGCCTGGAGGA 
               
               
                   
                 R42H, K48T, 
                 LKDPKKMPLLRL 
                 CGGTTATACACTGGGGGACTATAACATTCTTA 
               
               
                   
                 R54Y, S57G 
                 R 
                 AAGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM119 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [327; 977] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIFDHEGIP 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCTTC 
               
               
                   
                 H68M, E18M, 
                 PDQQHLTFQGTL 
                 GATCATGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                 N25V, Q31F, 
                 LEDGYTLGDYNIL 
                 ATCTTACTTTTCAAGGGACTTTGCTGGAGGAC 
               
               
                   
                 R42H, A44T, 
                 KDPKKMPLLRLR 
                 GGTTATACACTGGGGGACTATAACATTCTTAA 
               
               
                   
                 K48T, S49L, 
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                 R54Y, S57G 
                   
                 CGT 
               
               
                   
               
               
                 CM120 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [328; 978] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIFDHEGIP 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCTTC 
               
               
                   
                 H68M, E18M, 
                 LDQQHLAFQGTS 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 N25V, Q31F, 
                 LEDGYTLGDYNIL 
                 ATCTTGCTTTTCAAGGGACTAGCCTGGAGGA 
               
               
                   
                 P38L, R42H, 
                 KDPKKMPLLRLR 
                 CGGTTATACACTGGGGGACTATAACATTCTTA 
               
               
                   
                 K48T, R54Y, 
                   
                 AAGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                 S57G 
                   
                 GCGT 
               
               
                   
               
               
                 CM121 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [329; 979] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, E18M, 
                 PPDQQHLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 N25V, R42H, 
                 TSLEDGYTLGDY 
                 CATCTTGCTTTTCAAGGGACTAGCCTGGAGG 
               
               
                   
                 K48T, R54Y, 
                 NILKDPKKMPLLR 
                 ACGGTTATACACTGGGGGACTATAACATTCTT 
               
               
                   
                 S57G 
                 LR 
                 AAAGATCCTAAAAAGATGCCACTGCTGCGCT 
               
               
                   
                   
                   
                 TGCGT 
               
               
                   
               
               
                 CM131 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [330; 980] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 PDQQRLAFQGKS 
                 GATCATGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                 K63I, M1Y, 
                 LEDGRTLSDYNLL 
                 GCCTTGCTTTTCAAGGGAAGAGCCTGGAGGA 
               
               
                   
                 V26I, L73M 
                 IDPKKMPLLRMR 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                   
                   
                 TTGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM132 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [331; 981] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VIKAKIQDHEGIP 
                 ATACTATCGAGGTAATTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PDEQRLAFQGKS 
                 AGATCATGAAGGGATTCCTCCAGATGAGCAA 
               
               
                   
                 K63I, M1Y, 
                 LDDGRTLSDYNLL 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGATG 
               
               
                   
                 V26I, L73M, 
                 IDPKKMPLLRMR 
                 ACGGTCGCACACTGTCTGACTATAACTTGCTT 
               
               
                   
                 N25V, Q40E, 
                   
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCAT 
               
               
                   
                 E51D 
                   
                 GCGT 
               
               
                   
               
               
                 CM133 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [332; 982] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PPDEQRLAFQGK 
                 AGATCATGAAGGGATTCCTCCAGATGAGCAA 
               
               
                   
                 K63I, N25V, 
                 SLDDGRTLSDYN 
                 CGCCTTGCTTTTCAAGGGAAGAGCCTGGATG 
               
               
                   
                 Q40E, E51D 
                 LLIDPKKMPLLRL 
                 ACGGTCGCACACTGTCTGACTATAACTTGCTT 
               
               
                   
                   
                 R 
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM134 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [333; 983] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PPDQQRLAFQGT 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 K63I, E18M, 
                 SLDDGRTLGDYN 
                 CGCCTTGCTTTTCAAGGGACTAGCCTGGATGA 
               
               
                   
                 K48T, E51D, 
                 LLIDPKKMPLLRL 
                 CGGTCGCACACTGGGGGACTATAACTTGCTT 
               
               
                   
                 S57G 
                 R 
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM135 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [334; 984] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELMVEPSDTIE 
                 AAGATGATCGAGTTGATGGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PPDEQRLAFQGK 
                 AGATCATGAAGGGATTCCTCCAGATGAGCAA 
               
               
                   
                 K63I, E16M, 
                 LLEDGRTLSDYNL 
                 CGCCTTGCTTTTCAAGGGAAGTTGCTGGAGG 
               
               
                   
                 N25V, Q40E, 
                 LIDPKKMPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACTTGCTT 
               
               
                   
                 S49L 
                   
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM136 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [335; 985] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 PDQQRLAFQGTS 
                 GATCATGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                 K63I, E18M, 
                 LDDGRTLGDYNL 
                 GCCTTGCTTTTCAAGGGACTAGCCTGGATGAC 
               
               
                   
                 K48T, E51D, 
                 LIDPKKMPLLRM 
                 GGTCGCACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 S57G, M1Y, 
                 R 
                 TTGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                 V26I, L73M 
                   
                 CGT 
               
               
                   
               
               
                 CM137 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [336; 986] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 PDQQRLAFQGTS 
                 GATCATGAAGGGATTCCTCCAGATCAACAAC 
               
               
                   
                 K63I, E18M, 
                 LDDGRTLGDYNL 
                 GCCTTGCTTTTCAAGGGACTAGCCTGGATGAC 
               
               
                   
                 K48T, E51D, 
                 LIDPKKMPLLRM 
                 GGTCGCACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 S57G, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 R74Q 
                   
                   
               
               
                   
               
               
                 CM138 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [337; 987] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VIKAKIQDHEGIP 
                 ATACTATCGAGGTAATTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PDEQRLAFQGTS 
                 AGATCATGAAGGGATTCCTCCAGATGAGCAA 
               
               
                   
                 K63I, E18M, 
                 LDDGRTLGDYNL 
                 CGCCTTGCTTTTCAAGGGACTAGCCTGGATGA 
               
               
                   
                 K48T, E51D, 
                 LIDPKKMPLLRM 
                 CGGTCGCACACTGGGGGACTATAACTTGCTT 
               
               
                   
                 S57G, M1Y, 
                 Q 
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D 
                   
                   
               
               
                   
               
               
                 CM139 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [338; 988] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PPDQQRLTFQGK 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 K63I, A44T, 
                 LLEDGRTLSDYNL 
                 CGCCTTACTTTTCAAGGGAAGTTGCTGGAGG 
               
               
                   
                 S49L 
                 LIDPKKMPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACTTGCTT 
               
               
                   
                   
                   
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM140 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [339; 989] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PLDQQRLTFQGK 
                 AGATCATGAAGGGATTCCTTTGGATCAACAA 
               
               
                   
                 K63I, A44T, 
                 LLEDGRTLSDYNL 
                 CGCCTTACTTTTCAAGGGAAGTTGCTGGAGG 
               
               
                   
                 S49L, P38L 
                 LIDPKKMPLLRLR 
                 ACGGTCGCACACTGTCTGACTATAACTTGCTT 
               
               
                   
                   
                   
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM141 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [340; 990] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NVKAKIQDHEGI 
                 ATACTATCGAGAATGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PLDQQRLTFQGK 
                 AGATCATGAAGGGATTCCTTTGGATCAACAA 
               
               
                   
                 K63I, A44T, 
                 LLEDGRTLSDYNL 
                 CGCCTTACTTTTCAAGGGAAGTTGCTGGAGG 
               
               
                   
                 S49L, P38L, 
                 LIDPKKMPLLRLQ 
                 ACGGTCGCACACTGTCTGACTATAACTTGCTT 
               
               
                   
                 R74Q 
                   
                 ATTGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCAA 
               
               
                   
               
               
                 CM142 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [341; 991] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMPLLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                 V26I, L73M 
                   
                 CAA 
               
               
                   
               
               
                 CM143 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [342; 992] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PLDEQRLTFQGK 
                 AGATCATGAAGGGATTCCTTTGGATGAGCAA 
               
               
                   
                 K63I, A44T, 
                 LLDDGRTLSDYNL 
                 CGCCTTACTTTTCAAGGGAAGTTGCTGGATGA 
               
               
                   
                 S49L, P38L, 
                 LIDPKKMPLLRLQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, N25V, 
                   
                 TTGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                 Q40E, E51D 
                   
                 CAA 
               
               
                   
               
               
                 CM144 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [343; 993] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VIKAKIQDHEGIP 
                 ATACTATCGAGGTAATTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 LDEQRLTFQGKLL 
                 AGATCATGAAGGGATTCCTTTGGATGAGCAA 
               
               
                   
                 K63I, A44T, 
                 DDGRTLSDYNLLI 
                 CGCCTTACTTTTCAAGGGAAGTTGCTGGATGA 
               
               
                   
                 S49L, P38L, 
                 DPKKMPLLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, N25V, 
                   
                 TTGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                 Q40E, E51D, 
                   
                 CAA 
               
               
                   
                 M1Y, V26I, 
                   
                   
               
               
                   
                 L73M 
                   
                   
               
               
                   
               
               
                 CM145 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [344; 994] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PPDQQHLTFQGT 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 K63I, E18M, 
                 LLEDGYTLGDYNL 
                 CATCTTACTTTTCAAGGGACTTTGCTGGAGGA 
               
               
                   
                 N25V, R42H, 
                 LIDPKKMPLLRLR 
                 CGGTTATACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 K48T, R54Y, 
                   
                 TTGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                 S57G, A44T, 
                   
                 CGT 
               
               
                   
                 S49L 
                   
                   
               
               
                   
               
               
                 CM146 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [345; 995] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PLDQQHLTFQGT 
                 AGATCATGAAGGGATTCCTTTGGATCAACAA 
               
               
                   
                 K63I, E18M, 
                 LLEDGYTLGDYNL 
                 CATCTTACTTTTCAAGGGACTTTGCTGGAGGA 
               
               
                   
                 N25V, R42H, 
                 LIDPKKMPLLRLR 
                 CGGTTATACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 K48T, R54Y, 
                   
                 TTGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                 S57G, A44T, 
                   
                 CGT 
               
               
                   
                 S49L, P38L 
                   
                   
               
               
                   
               
               
                 CM147 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [346; 996] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PLDQQHLTFQGT 
                 AGATCATGAAGGGATTCCTTTGGATCAACAA 
               
               
                   
                 K63I, E18M, 
                 LLEDGYTLGDYNL 
                 CATCTTACTTTTCAAGGGACTTTGCTGGAGGA 
               
               
                   
                 N25V, R42H, 
                 LIDPKKMPLLRLQ 
                 CGGTTATACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 K48T, R54Y, 
                   
                 TTGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                 S57G, A44T, 
                   
                 CAA 
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q 
                   
                   
               
               
                   
               
               
                 CM148 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [347; 997] 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PLDEQHLTFQGT 
                 AGATCATGAAGGGATTCCTTTGGATGAGCAA 
               
               
                   
                 K63I, E18M, 
                 LLDDGYTLGDYN 
                 CATCTTACTTTTCAAGGGACTTTGCTGGATGA 
               
               
                   
                 N25V, R42H, 
                 LLIDPKKMPLLRL 
                 CGGTTATACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 K48T, R54Y, 
                 Q 
                 TTGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                 S57G, A44T, 
                   
                 CAA 
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D 
                   
                   
               
               
                   
               
               
                 CM149 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [348; 998] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VIKAKIQDHEGIP 
                 ATACTATCGAGGTAATTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 LDEQHLTFQGTL 
                 AGATCATGAAGGGATTCCTTTGGATGAGCAA 
               
               
                   
                 K63I, E18M, 
                 LDDGYTLGDYNL 
                 CATCTTACTTTTCAAGGGACTTTGCTGGATGA 
               
               
                   
                 N25V, R42H, 
                 LIDPKKMPLLRM 
                 CGGTTATACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 K48T, R54Y, 
                 Q 
                 TTGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                 S57G, A44T, 
                   
                 CAA 
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D, 
                   
                   
               
               
                   
                 M1Y, V26I, 
                   
                   
               
               
                   
                 L73M 
                   
                   
               
               
                   
               
               
                 CM199 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [349; 999] 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                   
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VIKAKIQDHEGIP 
                 ATACTATCGAGGTAATTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PDEQRLAFQGTS 
                 AGATCATGAAGGGATTCCTCCAGATGAGCAA 
               
               
                   
                 K63I, E18M, 
                 LDDGRTLGDYNL 
                 CGCCTTGCTTTTCAAGGGACTAGCCTGGATGA 
               
               
                   
                 K48T, E51D, 
                 LIDPKKMLVLRM 
                 CGGTCGCACACTGGGGGACTATAACTTGCTT 
               
               
                   
                 S57G, M1Y, 
                 Q 
                 ATTGATCCTAAAAAGATGTTGGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D, 
                   
                   
               
               
                   
                 P69L, L70V 
                   
                   
               
               
                   
               
               
                 CM203 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [350; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1000] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70V 
                   
                   
               
               
                   
               
               
                 CM204 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [351; 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1001] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PLDEQRLTFQGK 
                 AGATCATGAAGGGATTCCTTTGGATGAGCAA 
               
               
                   
                 K63I, A44T, 
                 LLDDGRTLSDYNL 
                 CGCCTTACTTTTCAAGGGAAGTTGCTGGATGA 
               
               
                   
                 S49L, P38L, 
                 LIDPKKMLVLRLQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, N25V, 
                   
                 TTGATCCTAAAAAGATGTTGGTACTGCGCTTG 
               
               
                   
                 Q40E, E51D, 
                   
                 CAA 
               
               
                   
                 P69L, L70V 
                   
                   
               
               
                   
               
               
                 CM208 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [352; 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1002] 
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PLDQQHLTFQGT 
                 AGATCATGAAGGGATTCCTTTGGATCAACAA 
               
               
                   
                 K63I, E18M, 
                 LLEDGYTLGDYNL 
                 CATCTTACTTTTCAAGGGACTTTGCTGGAGGA 
               
               
                   
                 N25V, R42H, 
                 LIDPKKMLVLRLQ 
                 CGGTTATACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 K48T, R54Y, 
                   
                 TTGATCCTAAAAAGATGTTGGTACTGCGCTTG 
               
               
                   
                 S57G, A44T, 
                   
                 CAA 
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q, P69L, 
                   
                   
               
               
                   
                 L70V 
                   
                   
               
               
                   
               
               
                 CM210 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [353; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1003] 
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VIKAKIQDHEGIP 
                 ATACTATCGAGGTAATTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 LDEQHLTFQGTL 
                 AGATCATGAAGGGATTCCTTTGGATGAGCAA 
               
               
                   
                 K63I, E18M, 
                 LDDGYTLGDYNL 
                 CATCTTACTTTTCAAGGGACTTTGCTGGATGA 
               
               
                   
                 N25V, R42H, 
                 LIDPKKMLVLRM 
                 CGGTTATACACTGGGGGACTATAACTTGCTTA 
               
               
                   
                 K48T, R54Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTGGTACTGCGCATG 
               
               
                   
                 S57G, A44T, 
                   
                 CAA 
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D, 
                   
                   
               
               
                   
                 M1Y, V26I, 
                   
                   
               
               
                   
                 L73M, P69L, 
                   
                   
               
               
                   
                 L70V 
                   
                   
               
               
                   
               
               
                 CM211 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [354; 
                 T12M, T14E, 
                 GMLIFVRMLTGK 
                 GCATGTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1004] 
                 K33H, A46Q, 
                 MIELEVMPSDTIE 
                 AAGATGATCGAGTTGGAAGTGATGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 VVKAKIQDHEGI 
                 ATACTATCGAGGTAGTTAAGGCCAAAATCCA 
               
               
                   
                 H68M, I61L, 
                 PPDQQHLAFQG 
                 AGATCATGAAGGGATTCCTCCAGATCAACAA 
               
               
                   
                 K63I, E18M, 
                 TSLEDGYTLGDY 
                 CATCTTGCTTTTCAAGGGACTAGCCTGGAGG 
               
               
                   
                 N25V, R42H, 
                 NLLIDPKKMLVLR 
                 ACGGTTATACACTGGGGGACTATAACTTGCTT 
               
               
                   
                 K48T, R54Y, 
                 LR 
                 ATTGATCCTAAAAAGATGTTGGTACTGCGCTT 
               
               
                   
                 S57G, P69L, 
                   
                 GCGT 
               
               
                   
                 L70V 
                   
                   
               
               
                   
               
               
                 CM358 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [355; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1005] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMAVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGGCCGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69A, L70V 
                   
                   
               
               
                   
               
               
                 CM359 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [356; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1006] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMRVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGCGCGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69R, L70V 
                   
                   
               
               
                   
               
               
                 CM360 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [357; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1007] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMNVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGAATGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69N, L70V 
                   
                   
               
               
                   
               
               
                 CM361 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [358; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1008] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMDVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGGATGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69D, L70V 
                   
                   
               
               
                   
               
               
                 CM362 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [359; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1009] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMCVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTGCGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69C, L70V 
                   
                   
               
               
                   
               
               
                 CM363 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [360; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1010] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMEVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGGAGGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69E, L70V 
                   
                   
               
               
                   
               
               
                 CM364 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [361; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1011] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMQVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGCAAGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69Q, L70V 
                   
                   
               
               
                   
               
               
                 CM365 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [362; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1012] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMGVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGGGGGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69G, L70V 
                   
                   
               
               
                   
               
               
                 CM366 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [363; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1013] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMHVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGCATGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69H, L70V 
                   
                   
               
               
                   
               
               
                 CM367 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [364; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1014] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMIVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGATTGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69I, L70V 
                   
                   
               
               
                   
               
               
                 CM368 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [365; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1015] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMKVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGAAGGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69K, L70V 
                   
                   
               
               
                   
               
               
                 CM369 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [366; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1016] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMMVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGATGGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69M, L70V 
                   
                   
               
               
                   
               
               
                 CM370 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [367; 
                 T12M,T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1017] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMFVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69F, L70V 
                   
                   
               
               
                   
               
               
                 CM371 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [368; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1018] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMSVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTCTGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69S, L70V 
                   
                   
               
               
                   
               
               
                 CM372 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [369; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1019] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMTVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGACTGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69T, L70V 
                   
                   
               
               
                   
               
               
                 CM373 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [370; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1020] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMWVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTGGGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69W, L70V 
                   
                   
               
               
                   
               
               
                 CM374 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [371; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1021] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMYVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTATGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69Y, L70V 
                   
                   
               
               
                   
               
               
                 CM375 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [372; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1022] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMVVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGGTAGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69V, L70V 
                   
                   
               
               
                   
               
               
                 CM376 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [373; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1023] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLALRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGGCCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70A 
                   
                   
               
               
                   
               
               
                 CM377 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [374; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1024] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLRLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGCGCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70R 
                   
                   
               
               
                   
               
               
                 CM378 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [375; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1025] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLNLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTGAATCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70N 
                   
                   
               
               
                   
               
               
                 CM379 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [376; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1026] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLDLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTGGATCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70D 
                   
                   
               
               
                   
               
               
                 CM380 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [377; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1027] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLCLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGTGCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70C 
                   
                   
               
               
                   
               
               
                 CM381 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [378; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1028] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLELRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGGAGCTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69L, L70E 
                   
                   
               
               
                   
               
               
                 CM382 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [379; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1029] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLQLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTGCAACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L700 
                   
                   
               
               
                   
               
               
                 CM383 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [380; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1030] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLGLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTGGGGCTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69L, L70G 
                   
                   
               
               
                   
               
               
                 CM384 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [381; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1031] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLHLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTGCATCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70H 
                   
                   
               
               
                   
               
               
                 CM385 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [382; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1032] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLILRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGATTCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70I 
                   
                   
               
               
                   
               
               
                 CM386 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [383; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1033] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLKLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGAAGCTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69L, L70K 
                   
                   
               
               
                   
               
               
                 CM387 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [384; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1034] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTGATGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70M 
                   
                   
               
               
                   
               
               
                 CM388 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [385; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1035] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLFLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGTTCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70F 
                   
                   
               
               
                   
               
               
                 CM389 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [386; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1036] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLPLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGCCTCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70P 
                   
                   
               
               
                   
               
               
                 CM390 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [387; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1037] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLSLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGTCTCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70S 
                   
                   
               
               
                   
               
               
                 CM391 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [388; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1038] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLTLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGACTCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70T 
                   
                   
               
               
                   
               
               
                 CM392 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [389; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1039] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLWLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTGATCCTAAAAAGATGTTGTGGCTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69L, L70W 
                   
                   
               
               
                   
               
               
                 CM393 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [390; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1040] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 IDPKKMLYLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCTTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTGATCCTAAAAAGATGTTGTATCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70Y 
                   
                   
               
               
                   
               
               
                 CM429 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [391; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1041] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM430 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [392; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1042] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMGVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGGGGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69G, L70V, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM431 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [393; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1043] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMLMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGTTGATGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69L, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM432 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [394; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1044] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCATGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM433 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [395; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1045] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAFLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCTTCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM434 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [396; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1046] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMACLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCTGCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM435 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [397; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1047] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMGMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGGGATGCTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69G, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM436 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [398; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1048] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMGFLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGGGTTCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69G, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM437 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [399; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1049] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMGCLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGGGTGCCTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69G, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM438 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [400; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1050] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMCMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGTGCATGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69C, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM439 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [401; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1051] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMMMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGATGATGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69M, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM440 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [402; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1052] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMFMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGTTCATGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69F, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM441 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [403; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1053] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 AISPEKMAVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGGCCA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62A, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM442 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [404; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1054] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLR 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCGCA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62R, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM443 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [405; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1055] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 NISPEKMAVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGAATA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62N, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM444 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [406; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1056] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 DISPEKMAVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGGATA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62D, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM445 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [407; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1057] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLC 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGTGCA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62C, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM446 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [408; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1058] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLE 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGGAG 
               
               
                   
                 R74Q, M1Y, 
                   
                 ATTTCTCCTGAGAAGATGGCCGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62E, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM447 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [409; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1059] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 QISPEKMAVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCAAA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L620, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM448 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [410; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1060] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 GISPEKMAVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGGGG 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 ATTTCTCCTGAGAAGATGGCCGTACTGCGCAT 
               
               
                   
                 V26I, L73M, 
                   
                 GCAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62G, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM449 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [411; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1061] 
                 K33H, A46Q., 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 HISPEKMAVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCATA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62H, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM450 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [412; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1062] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLII 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 SPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGATTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62I, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM451 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [413; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1063] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLK 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGAAGA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62K, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM452 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [414; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1064] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 MISPEKMAVLR 
                 CGGTCGCACACTGTCTGACTATAACTTGATGA 
               
               
                   
                 R74Q, M1Y, 
                 MQ 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62M, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM453 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [415; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1065] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLF 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGTTCA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62F, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM454 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [416; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1066] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLS 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGTCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62S, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM455 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [417; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1067] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM456 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [418; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1068] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 WISPEKMAVLR 
                 CGGTCGCACACTGTCTGACTATAACTTGTGGA 
               
               
                   
                 R74Q, M1Y, 
                 MQ 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62W, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM457 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [419; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1069] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLY 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGTATA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62Y, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM458 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [420; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1070] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 VISPEKMAVLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGGTAA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62V, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM459 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [421; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1071] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMCFLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGTGCTTCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69C, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM460 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [422; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1072] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMMFLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGATGTTCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69M, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM461 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [423; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1073] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMFFLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGTTCTTCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69F, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM462 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [424; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1074] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMCCLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGTGCTGCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69C, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM463 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [425; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1075] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMMCLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGATGTGCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69M, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM464 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [426; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1076] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLP 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMFCLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGCCTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGATGTGCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69F, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM465 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [427; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1077] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 AISPEKMAMLR 
                 CGGTCGCACACTGTCTGACTATAACTTGGCCA 
               
               
                   
                 R74Q, M1Y, 
                 MQ 
                 TTTCTCCTGAGAAGATGATGTGCCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62A, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM467 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [428; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1078] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLC 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGTGCA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCATGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62C, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM468 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [429; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1079] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAMLRM 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCATGCTTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM469 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCACCACCACCACCACGGTGGATCTG 
               
               
                 [430; 
                 T12M, T14E, 
                 GYMIFVRMLTGK 
                 GCTATATGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1080] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNL 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 VISPEKMAMLR 
                 CGGTCGCACACTGTCTGACTATAACTTGGTAA 
               
               
                   
                 R74Q, M1Y, 
                 MQ 
                 TTTCTCCTGAGAAGATGGCCATGCTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62V, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM478 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [431; 
                 T12M, T14E, 
                 GYAIFVRMLTGK 
                 GCTATGCCATTTTCGTACGCATGTTGACTGGA 
               
               
                 1081] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2A, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM479 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [432; 
                 T12M, T14E, 
                 GYRIFVRMLTGK 
                 GCTATCGCATTTTCGTACGCATGTTGACTGGA 
               
               
                 1082] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2R, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM480 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [433; 
                 T12M, T14E, 
                 GYNIFVRMLTGK 
                 GCTATAATATTTTCGTACGCATGTTGACTGGA 
               
               
                 1083] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2N, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM481 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [434; 
                 T12M, T14E, 
                 GYDIFVRMLTGK 
                 GCTATGAIATTTTCGTACGCATGTTGACTGGA 
               
               
                 1084] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2D, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM482 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [435; 
                 T12M, T14E, 
                 GYCTFVRMLTGK 
                 GCTATTGCATTTTCGTACGCATGTTGACTGGA 
               
               
                 1085] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2C, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM483 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [436; 
                 T12M, T14E, 
                 GYEIFVRMLTGK 
                 GCTATGAGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1086] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2E, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM484 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [437; 
                 T12M, T14E, 
                 GYQIFVRMLTGK 
                 GCTATCAAATTTTCGTACGCATGTTGACTGGA 
               
               
                 1087] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L20, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM485 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [438; 
                 T12M, T14E, 
                 GYGIFVRMLTGK 
                 GCTATGGGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1088] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2G, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM486 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [439; 
                 T12M, T14E, 
                 GYHIFVRMLTGK 
                 GCTATCATATTTTCGTACGCATGTTGACTGGA 
               
               
                 1089] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K631, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2H, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM487 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [440; 
                 T12M, T14E, 
                 GYIIFVRMLTGK 
                 GCTATATTATTTTCGTACGCATGTTGACTGGA 
               
               
                 1090] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2I, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM488 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [441; 
                 T12M, T14E, 
                 GYKIFVRMLTGK 
                 GCTATAAGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1091] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2K, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM489 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [442; 
                 T12M, T14E, 
                 GYLIFVRMLTGK 
                 GCTATTTGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1092] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L62T, D64S, 
                   
                   
               
               
                   
                 K66E 
                   
                   
               
               
                   
               
               
                 CM490 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [443; 
                 T12M, T14E, 
                 GYFIFVRMLTGK 
                 GCTATTTCATTTTCGTACGCATGTTGACTGGA 
               
               
                 1093] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2F, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM491 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [444; 
                 T12M, T14E, 
                 GYSIFVRMLTGK 
                 GCTATTCTATTTTCGTACGCATGTTGACTGGA 
               
               
                 1094] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2S, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM492 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [445; 
                 T12M, T14E, 
                 GYTIFVRMLTGK 
                 GCTATACTATTTTCGTACGCATGTTGACTGGA 
               
               
                 1095] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2T, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM493 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [446; 
                 T12M, T14E, 
                 GYWIFVRMLTGK 
                 GCTATTGGATTTTCGTACGCATGTTGACTGGA 
               
               
                 1096] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2W, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM494 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [447; 
                 T12M, T14E, 
                 GYYIFVRMLTGK 
                 GCTATTATATTTTCGTACGCATGTTGACTGGA 
               
               
                 1097] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2Y, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM495 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [448; 
                 T12M, T14E, 
                 GYVIFVRMLTGK 
                 GCTATGTAATTTTCGTACGCATGTTGACTGGA 
               
               
                 1098] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2V, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM496 
                 K6R, T7M, 
                 MHHHHHHGGS 
                 ATGCACCATCACCACCACCACGGTGGATCTG 
               
               
                 [449; 
                 T12M, T14E, 
                 GYPIFVRMLTGK 
                 GCTATCCTATTTTCGTACGCATGTTGACTGGA 
               
               
                 1099] 
                 K33H, A46Q, 
                 MIELEVEPSDTIE 
                 AAGATGATCGAGTTGGAAGTGGAGCCTTCCG 
               
               
                   
                 S65P, L67K, 
                 NIKAKIQDHEGIP 
                 ATACTATCGAGAATATTAAGGCCAAAATCCAA 
               
               
                   
                 H68M, I61L, 
                 LDQQRLTFQGKL 
                 GATCATGAAGGGATTCCTTTGGATCAACAAC 
               
               
                   
                 K63I, A44T, 
                 LEDGRTLSDYNLT 
                 GCCTTACTTTTCAAGGGAAGTTGCTGGAGGA 
               
               
                   
                 S49L, P38L, 
                 ISPEKMAVLRMQ 
                 CGGTCGCACACTGTCTGACTATAACTTGACTA 
               
               
                   
                 R74Q, M1Y, 
                   
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 V26I, L73M, 
                   
                 CAA 
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2P, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
               
               
                   
               
               
                   a The SEQ ID NOS shown in brackets correspond to the protein amino acid SEQ ID NO, followed by the DNA nucleic acid SEQ ID No. 
               
            
           
         
       
     
     Example 5. Ubiquitin Variants Targeting 53BP1 Provide an Additional Benefit to HDR when Used in Conjunction with NHEJ Inhibitors 
     To test if ubiquitin variants targeting 53BP1 provide a benefit when used in conjunction with small molecule inhibitors reported to boost HDR we tested if the rate of HDR using a DNA-dependent protein kinase (DNA-PK) inhibitor, IDT Enhancer (IDT-E or Alt-R HDR Enhancer), was further increased by using it in combination with CM1. DNA-PK is a critical protein complex in the NHEJ pathway, by inhibiting DNA-PK these small molecules bias the cell towards use of homologous recombination instead of NHEJ to repair double strand breaks induced by CRISPR/Cas9 and other nucleases thereby facilitating gene editing. Notably, 53BP1 recruitment is not dependent on the kinase activity of DNA-PK and is instead recruited through an ATM dependent pathway [29, 30]. Further, 53BP1 recruitment and formation of 53BP1 foci is often used to visualize the presence of double strand breaks, including in the presence of DNA-PK inhibitors which can cause 53BP1 foci to persist for a greater period due to inhibition of the normally rapid repair through the NHEJ pathway [27, 31]. We hypothesized that inhibition of 53BP1 may provide an additional benefit when used in conjunction with inhibitors of common NHEJ pathway targets such as DNA-PK and DNA-ligase IV due to the ability of inhibitors of 53BP1 to enhance HDR not just through a negative effect on NHEJ but also promoting HDR by facilitating end resection. 
     We tested if our ubiquitin variants provided a further benefit over inhibition of common NHEJ pathway targets alone by using the DNA-PK inhibitor IDT enhancer (IDT-E) in combination with CM1 in the context of both large and small inserts (Table 7). The results are shown in  FIG.  12   . Both IDT-E and CM1 were able to individual increase rates of HDR using both donors types, however higher HDR rates were achieved when both were used together than either inhibitor alone. Without limiting the claimed subject matter to a particular mode or mechanism of action, we hypothesize that our ubiquitin variants targeting 53BP1 will be a useful in facilitating increased HIDR when used in combination with other inhibitors of NHEJ pathway components. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Gene, protospacer, targets, and donor sequences. 
               
            
           
           
               
               
               
               
            
               
                 Gene 
                   
                 Coor- 
                   
               
               
                 [SEQ ID 
                   
                 dinates 
                   
               
               
                 NO:] a   
                 protospacer 
                 (hg38) 
                 Donor Sequence 
               
               
                   
               
               
                 SERPINC1 
                 ACCTCTG 
                 chr1: 
                 /Alt-R-HDR1/A*T*TCCAATGTGATAGGAACTGTAACCTCTGGA 
               
               
                 [1101; 
                 GAAAAAG 
                 173,917,213- 
                 AAAAGGTAGAATTCAGAGGGGTGAGCTTTCCCCTTGCCTGC 
               
               
                 1103] 
                 GTAAGA 
                 173, 917,232 
                 CCCTACTGGGT*T*T/Alt-R-HDR2/ 
               
               
                   
               
               
                 MET 
                 CAAAGTCC 
                 chr7: 
                 /Alt-R-HDR1/T*G*TGTGGTGAGCGCCCTGGGAGCCAAAGTCC 
               
               
                 [1104; 
                 TTTCATCTG 
                 116,699,630- 
                 TTTCATCTGGAATTCTAAAGGACCGGTTCATCAACTTCTTTG 
               
               
                 1105] 
                 TAA 
                 116,699,649 
                 TAGGCAATACC*A*T/Alt-R-HDR2/ 
               
               
                   
               
               
                 HPRT1 
                 AATTATGG 
                 chrX: 
                 /Alt-R-HDR1/A*A*AGACTATGAAATGGAGAGCTAAATTATGGGGA 
               
               
                 [1106; 
                 GGATTACT 
                 134,498,212- 
                 TTACTAGAATTCGGAAGGGGCAGCAATGAGTTGACACTACAGACA 
               
               
                 1107] 
                 AGGA 
                 134,498,231 
                 AGGCA*C*T/Alt-R-HDR2/ 
               
               
                   
               
               
                 CLTA 
                 GAACGGA 
                 chr9: 
                 GTCGTACCGACTGGTAGATGACAGCAAACCTGTTCCCTTTTCGGCTC 
               
               
                 [1108; 
                 TCCAGCT 
                 36,191,058- 
                 TGCAACACCGCCTAGACCGACCGGATACACGGGTAGGGCTTCCGCT 
               
               
                 1109] 
                 CAGCCA 
                 36,191,077 
                 TTACCCGTCTCCCTCCTGGCGCTTGTCCTCCTCTCCCAGTCGGCACCA 
               
               
                   
                   
                   
                 CAGCGGTGGCTGCCGGGCGTGGTGTCGGTGGGTCGGTTGGTTTTT 
               
               
                   
                   
                   
                 GTCTCACCGTTGGTGTCCGTGCCGTTCAGTTGCCCGCCATGGCTGG 
               
               
                   
                   
                   
                 ATCTGGTGGTACTAGTGGAAGCAAGGGTGAGGAGCTGTTCACCGG 
               
               
                   
                   
                   
                 AGTGGTGCCTATCCTGGTCGAGCTGGACGGCGACGTAAACGGTCA 
               
               
                   
                   
                   
                 CAAGTTCAGCGTGCGTGGTGAGGGCGAGGGCGATGCCACCAACGG 
               
               
                   
                   
                   
                 CAAGCTGACCCTGAAGTTCATCTGCACCACTGGCAAGCTGCCTGTTC 
               
               
                   
                   
                   
                 CATGGCCAACCCTCGTGACTACACTGACCTACGGCGTTCAGTGCTTC 
               
               
                   
                   
                   
                 AGCCGTTACCCTGACCATATGAAGCGTCACGACTTCTTCAAGTCTGC 
               
               
                   
                   
                   
                 CATGCCTGAAGGCTACGTCCAGGAGCGTACCATCAGCTTCAAGGAC 
               
               
                   
                   
                   
                 GATGGCACCTACAAGACTCGTGCCGAGGTGAAGTTCGAGGGTGAC 
               
               
                   
                   
                   
                 ACCCTGGTGAACCGCATCGAGCTGAAGGGTATCGACTTCAAGGAG 
               
               
                   
                   
                   
                 GACGGCAACATCCTGGGTCACAAGCTGGAGTACAACTTCAACAGCC 
               
               
                   
                   
                   
                 ACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGG 
               
               
                   
                   
                   
                 CCAACTTCAAGATTCGTCACAACGTGGAGGACGGTAGCGTGCAGCT 
               
               
                   
                   
                   
                 CGCAGACCACTACCAGCAGAACACGCCTATCGGCGACGGTCCAGTG 
               
               
                   
                   
                   
                 TTGCTGCCAGACAACCACTACCTGAGCACCCAGTCCGTGCTGAGCA 
               
               
                   
                   
                   
                 AAGACCCGAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCG 
               
               
                   
                   
                   
                 TGACCGCAGCCGGTATCACTGGAACCGGTGCTGGAAGTGGTGAGC 
               
               
                   
                   
                   
                 TGGATCCGTTCGGCGCCCCTGCCGGCGCCCCTGGCGGTCCCGCGCT 
               
               
                   
                   
                   
                 GGGGAACGGAGTGGCCGGCGCCGGCGAAGAAGACCCGGCTGCGG 
               
               
                   
                   
                   
                 CCTTCTTGGCGCAGCAAGAGAGCGAGATTGCGGGCATCGAGAACG 
               
               
                   
                   
                   
                 ACGAGGCCTTCGCCATCCTGGACGGCGGCGCCCCCGGGCCCCAGC 
               
               
                   
                   
                   
                 CGCACGGCGAGCCGCCGATCCGAAAACGGGCGTATAGTCGAGACC 
               
               
                   
               
               
                   a The SEQ D NOS shown in brackets correspond to the protospacer SEQ ID NO, followed by the Donor Seq uence SEQ ID NO. 
               
            
           
         
       
     
     Example 6. Screening of Amino Acid Substitutions at Position 2 Reveals an Additional Beneficial Mutation at Position 2 
     Testing of additional mutations identified a variant with improved affinity over that of the previously described CM455. In order to determine if the amino acid change made at position 2 (L2M) in CM455 relative to i53 was the optimal amino acid change at that position, we screened additional amino acid changes for their effect on the affinity for binding 53BP1. The results are shown in  FIG.  13   . The fold change in affinity is measured as the association constant (KA) of the ubiquitin variant being tested, divided by the KA of the reference ubiquitin variant (CM489), as determined by calculating each affinity for binding a fragment of 53BP1 using biolayer interferometry (BLI). The BLI steady-state response versus 53BP1 fragment concentration was plotted in prism to calculate the Kd using a one site-specific binding nonlinear fit model. If the affinity of a ubiquitin variant being tested is higher (binding is tighter) than for the reference ubiquitin variant, then the fold change in affinity will be &gt;1. Of the mutations tested, the majority were shown to be detrimental, resulting in worse affinity for 53BP1 than CM455. Compared to CM489 which has the original Q2L mutation at position 2 (relative to WT ubiquitin), the L2M mutation (Q2M relative to wild-type ubiquitin) identified from our previously described screen as the least detrimental mutation at position 2 provides a similar level of affinity as the Q2L mutation, however our L2I mutation (Q2I relative to WT ubiquitin) results in higher affinity than the L2M of CM455. Therefore, switching from L2M to L2I in CM455 may result in a ubiquitin variant (CM487) with improved ability to enhance rates of HDR. 
     Example 7. Tag-Free CM1 (CM1tf) Boosts HDR to the Same Degree as 6×His-Tagged CM1 
     A tag-free version of CM1 (CM1tf, SEQ ID NO:482) was compared with the His6-tagged version of CM1 (SEQ ID NO:241) for their ability to enhance HDR in HEK293 cells as has been described in previous examples. Briefly, 2 uM Cas9 RNP targeting a site in HPRT1 and 2 uM ssDNA donor containing 40 bp homology arms flanking a 6 bp EcoR1 cut site insert sequence were delivered into HEK293 cells with varying amounts of CM1tf (CM1tf, SEQ ID NO:482) or His-tagged CM1 (CM1; SEQ ID NO:241) using Lonza nucleofection. Genomic DNA was isolated after 48 hours, and editing was measured using an EcoR1 cleavage assay. The results are shown in  FIG.  15   . We found that the ability of the CM1 variant lacking a His-tag (CM1tf, SEQ ID NO:482) to enhance HDR is equivalent to that of His-tagged CM1 (CM1; SEQ ID NO:241). 
     Example 8. Mode of Delivery of an Ubv Via mRNA or Vector-Mediated Expression is Effective at Enhancing HDR Rates 
     In order to test if CM1 is effective at increasing HDR rates when delivered in other forms, plasmid or mRNA encoding CM1 was introduced into cells and the effects on HDR rates were analyzed. To test the effectiveness of CM1 delivered as plasmid, 154 ng of plasmid encoding His-tagged i53, His-tagged CM1, or a crRNA for LbCas12a was co-delivered with 154 ng of plasmid encoding sgRNA targeting HPRT1 into Jurkat cells by Lonza nucleofection using SF buffer and program DS-150. After 72 hours, genomic DNA was extracted using QuickExtract (Lucigen) and editing was analyzed by PCR amplification of the HPRT1 target site followed by EcoR1 restriction enzyme digestion. Digested product was run on a Fragment Analyzer (AATI). The results are shown in  FIG.  16 A . 
     Use of plasmid encoding i53 or CM1 resulted in an increase in HDR rates, with CM1 causing a larger increase in HDR rate. In order to test if CM1 is effective when delivered as mRNA, mRNA encoding CM1tf or CM1tf protein (12.5 μM) was delivered with 2 μM Cas9 RNP targeting HPRT1 and 2 μM HPRT1 EcoR1 cut site ssDNA donor by Lonza nucleofection (SE solution, pulse code CL-120). The indicated mRNA concentration (6.56 nM) was calculated using the commonly used 40 ug/ml for an OD260 of 1 absorbance estimate for ssRNA. Using a sequence specific extinction coefficient, the concentration was calculated as 4.61 nM. After 48 hours genomic DNA was extracted and the rate of HDR was analyzed as described previously. The results are shown in  FIG.  16 B . 
     Introduction of CM1tf as either protein or mRNA provided a similar level of boost in HDR rates over the no enhancer control. No additional benefit was observed when CM1tf mRNA and protein were added together, however there may be some benefit to adding them in combination in other cell types or with other types of donor DNA. The CM1tf mRNA was generated from PCR product from a human codon optimized CM1tf expression vector (made by IDT) using the HiScribe T7 ARCA kit (NEB) and Monarch RNA cleanup columns (NEB). The poly-A tail was encoded in the PCR product by addition of a poly-T sequence to the reverse primer (Table 8). 
     
       
         
           
               
             
               
                 TABLE 8 
               
               
                   
               
               
                 Sequences associated with CMltf mRNA production: 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 reverse primer to 
                 TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 
               
               
                 generate DNA 
                 TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 
               
               
                 template for mRNA 
                 TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAAGGCACAGT 
               
               
                 production 
                 CGAGGCT 
               
               
                 [SEQ ID NO: 1110] 
                   
               
               
                   
               
               
                 Forward primer to 
                 CCACTGCTTACTGGCTTATCGAAAT 
               
               
                 generate DNA 
                   
               
               
                 template for mRNA 
                   
               
               
                 production 
                   
               
               
                 [SEQ ID NO: 1111] 
                   
               
               
                   
               
               
                 PCR amplified 
                 CCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATA G GGAGAC 
               
               
                 sequence 
                 CCAAGCTGGCTAGCGTTTAAACGGGCCCTCTAGACTCGAGCGGCCGCC 
               
               
                 (double underline 
                 ACC ATGCTGATCTTCGTGAGAATGCTGACCGGCAAGATGATCGAACTG   
               
               
                 indicates transcription 
                 
                   GAAGTGGAACCCAGCGACACCATCGAGAACGTGAAGGCCAAAATCCAG 
                 
               
               
                 start site) 
                 
                   GACCACGAGGGCATCCCTCCTGACCAGCAGAGACTGGCCTTTCAGGGA 
                 
               
               
                 (underlined region is 
                 
                   AAGTCCCTGGAAGATGGAAGAACCCTGAGCGACTACAACATCCTGAAG 
                 
               
               
                 the open reading 
                   GACCCTAAGAAGATGCCACTGCTGAGACTGAGATGA TCAGCCTCGACT 
               
               
                 frame for CM1tf) 
                 GTGCCTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 
               
               
                 [SEQ ID NO: 1112] 
                 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 
               
               
                   
                 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 
               
               
                   
               
            
           
         
       
     
     Example 9. Sequences 
     A summary of amino acid and DNA sequences is presented in Table 9. 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Summary of Ubiquitin, i53 and Tag-free versions of Ubvs Sequences 
               
            
           
           
               
               
               
               
            
               
                 Name 
                 Amino acid 
                   
                   
               
               
                 [SEQ ID 
                 changes 
                 Protein 
                   
               
               
                 NOS] a   
                 relative to i53 
                 Sequence 
                 DNA sequence 
               
               
                   
               
               
                 Ubiquitin 
                 C-terminal GG 
                 MQIFVKTLTG 
                 ATGCAGATTTTCGTGAAAACCCTTACGGGGA 
               
               
                 [1; 666] 
                 Q2L, I44A, 
                 KTITLEVEPS 
                 AGACCATCACCCTCGAGGTTGAACCCTCGGA 
               
               
                   
                 Q49S, Q62L, 
                 DTIENVKAKI 
                 TACGATAGAAAATGTAAAGGCCAAGATCCAG 
               
               
                   
                 E64D, T66K, 
                 QDKEGIPPDQ 
                 GATAAGGAAGGAATTCCTCCTGATCAGCAGA 
               
               
                   
                 L69P, and V70L 
                 QRLIFAGKQL 
                 GACTGATCTTTGCTGGCAAGCAGCTGGAAGA 
               
               
                   
                   
                 EDGRTLSDYN 
                 TGGACGTACTTTGTCTGACTACAATATTCAAA 
               
               
                   
                   
                 IQKESTLHLV 
                 AGGAGTCTACTCTTCATCTTGTGTTGAGACTT 
               
               
                   
                   
                 LRLRGG 
                 CGTGGTGGT 
               
               
                   
               
               
                 i53 
                 None 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [2; 667] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 i53 DM 
                 P69L, L70V 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [451; 668] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHLVLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCTGGTTCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 i53 K6R 
                 K6R 
                 MLIFVRTLTGKTI 
                 ATGTTGATTTTCGTACGCACGTTGACTGGAAA 
               
               
                 [452; 669] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 i53 T14E 
                 T14E 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [453; 670] 
                   
                 ELEVEPSDTIENV 
                 GACTATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 ATAAGGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CCTTGCTTTTGCCGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 SKLHPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATTCTAAATTGCATCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 i53 K33A 
                 K33A 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [454; 671] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDAEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TGCCGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 i53 A46Q 
                 A46Q 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [455; 672] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFQGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTCAAGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 i53 K63I 
                 K63I 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [456; 673] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILID 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTATT 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 i53 S65P 
                 S65P 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [457; 674] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 PKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATCCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM1 
                 M1H 
                 HLIFVKTLTGKTIT 
                 CATTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [458; 675] 
                   
                 LEVEPSDTIENVK 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 AKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM2 
                 K6R 
                 MLIFVRTLTGKTI 
                 ATGTTGATTTTCGTACGCACGTTGACTGGAAA 
               
               
                 [459; 676] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM3 
                 T7M 
                 MLIFVKMLTGKTI 
                 ATGTTGATTTTCGTAAAGATGTTGACTGGAAA 
               
               
                 [460; 677] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM4 
                 T12M 
                 MLIFVKTLTGKMI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [461; 678] 
                   
                 TLEVEPSDTIENV 
                 GATGATCACTTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 ATAAGGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CCTTGCTTTTGCCGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 SKLHPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATTCTAAATTGCATCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 SM5 
                 T14E 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [462; 679] 
                   
                 ELEVEPSDTIENV 
                 GACTATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 ATAAGGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CCTTGCTTTTGCCGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 SKLHPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATTCTAAATTGCATCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 SM6 
                 E16M 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [463; 680] 
                   
                 TLMVEPSDTIEN 
                 GACTATCACTTTGATGGTGGAGCCTTCCGATA 
               
               
                   
                   
                 VKAKIQDKEGIPP 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM7 
                 E18M 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [464; 681] 
                   
                 TLEVMPSDTIEN 
                 GACTATCACTTTGGAAGTGATGCCTTCCGATA 
               
               
                   
                   
                 VKAKIQDKEGIPP 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM8 
                 N25V 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [465; 682] 
                   
                 TLEVEPSDTIEVV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGGTAGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM9 
                 V26I 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [466; 683] 
                   
                 TLEVEPSDTIENIK 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 AKIQDKEGIPPD 
                 CTATCGAGAATATTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM10 
                 Q31W 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [467; 684] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIWDKEGIPP 
                 CTATCGAGAATGTTAAGGCCAAAATCTGGGA 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM11 
                 Q310 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [468; 685] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKICDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCTGCGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM12 
                 Q31F 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [469; 686] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIFDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCTTCGAT 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 AAGGAAGGGATTCCTCCAGATCAACAACGCC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 TTGCTTTTGCCGGGAAGAGCCTGGAGGACGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 TCGCACACTGTCTGACTATAACATTCTTAAAG 
               
               
                   
                   
                   
                 ATTCTAAATTGCATCCACTGCTGCGCTTGCGT 
               
               
                   
               
               
                 SM13 
                 K33S 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [470; 687] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDSEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TTCTGAAGGGATTCCTCCAGATCAACAACGCC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 TTGCTTTTGCCGGGAAGAGCCTGGAGGACGG 
               
               
                   
                   
                 SKLHPLLRLR 
                 TCGCACACTGTCTGACTATAACATTCTTAAAG 
               
               
                   
                   
                   
                 ATTCTAAATTGCATCCACTGCTGCGCTTGCGT 
               
               
                   
               
               
                 SM14 
                 K33H 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [471; 688] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDHEGIPP 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 DQQRLAFAGKSL 
                 TCATGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM15 
                 K33A 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [472; 689] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDAEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TGCCGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM16 
                 P38L 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [473; 690] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPLD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTTTGGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM17 
                 R42W 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [474; 691] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQWLAFAGKSL 
                 TAAGGAAGGGATTCCTCCAGATCAACAATGG 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM18 
                 A44T 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [475; 692] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLTFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTACTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM19 
                 A46Q 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [476; 693] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFQGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTCAAGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM20 
                 K48T 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [477; 694] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGTSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGACTAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM21 
                 R54Y 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [478; 695] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGYTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTTATACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM22 
                 S57G 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [479; 696] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLGDYNILK 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 DSKLHPLLRLR 
                 GTCGCACACTGGGGGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATTCTAAATTGCATCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 SM23 
                 K63I 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [480; 697] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILID 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 SKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTATT 
               
               
                   
                   
                   
                 GATTCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 SM24 
                 S65P 
                 MLIFVKTLTGKTI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [481; 698] 
                   
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 QQRLAFAGKSLE 
                 TAAGGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CTTGCTTTTGCCGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 PKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATCCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 CM1tf 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [482; 699] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM7 
                 K6R, K33H, 
                 MLIFVRTLTGKTI 
                 ATGTTGATTTTCGTACGCACGTTGACTGGAAA 
               
               
                 [483; 700] 
                 A46Q, S65P 
                 TLEVEPSDTIENV 
                 GACTATCACTTTGGAAGTGGAGCCTTCCGATA 
               
               
                   
                   
                 KAKIQDHEGIPP 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                   
                 DQQRLAFQGKSL 
                 TCATGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CTTGCTTTTCAAGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 DPKLHPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATCCTAAATTGCATCCACTGCTGCGCTTGCG 
               
               
                   
                   
                   
                 T 
               
               
                   
               
               
                 CM13 
                 T7M, T14E, 
                 MLIFVKMLTGKTI 
                 ATGTTGATTTTCGTAAAGATGTTGACTGGAAA 
               
               
                 [484; 701] 
                 A46Q, L67K 
                 ELEVEPSDTIENV 
                 GACTATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                   
                 KAKIQDKEGIPPD 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                   
                 QQRLAFQGKSLE 
                 ATAAGGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 DGRTLSDYNILKD 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 SKKHPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATTCTAAAAAGCATCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM26 
                 T12M, K33H, 
                 MLIFVKTLTGKMI 
                 ATGTTGATTTTCGTAAAGACGTTGACTGGAAA 
               
               
                 [485; 702] 
                 A46q, H68M 
                 TLEVEPSDTIENV 
                 GATGATCACTTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                   
                 KAKIQDHEGIPP 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                   
                 DQQRLAFQGKSL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 DSKLMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATTCTAAATTGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM44 
                 T7M, T12M, 
                 MLIFVKMLTGK 
                 ATGTTGATTTTCGTAAAGATGTTGACTGGAAA 
               
               
                 [486; 703] 
                 T14E, K33H, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 A46Q, S65P, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 L67K, H68M 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM45 
                 K6R, T12M, 
                 MLIFVRTLTGKMI 
                 ATGTTGATTTTCGTACGCACGTTGACTGGAAA 
               
               
                 [487; 704] 
                 T14E, K33H, 
                 ELEVEPSDTIENV 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 A46Q, S65P, 
                 KAKIQDHEGIPP 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 L67K, H68M 
                 DQQRLAFQGKSL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 DPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM46 
                 K6R, T7M, 
                 MLIFVRMLTGKTI 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [488; 705] 
                 T14E, K33H, 
                 ELEVEPSDTIENV 
                 GACTATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 A46Q, S65P, 
                 KAKIQDHEGIPP 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 L67K, H68M 
                 DQQRLAFQGKSL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 EDGRTLSDYNILK 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 DPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM47 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [489; 706] 
                 T12M, K33H, 
                 MITLEVEPSDTIE 
                 GATGATCACTTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 A46Q, S65P, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 L67K, H68M 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM48 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [490; 707] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 A46Q, S65P, 
                 NVKAKIQDKEGIP 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 L67K, H68M 
                 PDQQRLAFQGKS 
                 ATAAGGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 LEDGRTLSDYNIL 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM49 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [491; 708] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, S65P, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 L67K, H68M 
                 PPDQQRLAFAGK 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 SLEDGRTLSDYNI 
                 CCTTGCTTTTGCCGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                 CM50 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [492; 709] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 L67K, H68M 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDSKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATTCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM51 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [493; 710] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, H68M 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKLMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAATTGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM52 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [494; 711] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                   
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKHPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGCATCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM62 
                 K6R, T7M, 
                 HLIFVRMLTGKM 
                 CATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [495; 712] 
                 T12M, T14E, 
                 IELEVEPSDTIENV 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPP 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 DQQRLAFQGKSL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, MIH 
                 EDGRTLSDYNILK 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 DPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM63 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [496; 713] 
                 T12M, T14E, 
                 ELEVEPSDTIENV 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPP 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 DQQRLAFQGKSL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, M1Y 
                 EDGRTLSDYNILK 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 DPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM64 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [497; 714] 
                 T12M, T14H, 
                 MIHLEVEPSDTIE 
                 GATGATCCATTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM65 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [498; 715] 
                 T12M, T14D, 
                 MIDLEVEPSDTIE 
                 GATGATCGATTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM66 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [499; 716] 
                 T12M, T14E, 
                 MIELMVEPSDTIE 
                 GATGATCGAGTTGATGGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, E16M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM67 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [500; 717] 
                 T12M, T14E, 
                 MIELTVEPSDTIE 
                 GATGATCGAGTTGACTGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, E16T 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM68 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [501; 718] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, E18M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM69 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [502; 719] 
                 T12M, T14E, 
                 MIELEVYPSDTIE 
                 GATGATCGAGTTGGAAGTGTATCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, E18Y 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM70 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [503; 720] 
                 T12M, T14E, 
                 MIELEVLPSDTIE 
                 GATGATCGAGTTGGAAGTGTTGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, E18L 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM71 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [504; 721] 
                 T12M, T14E, 
                 MIELEVFPSDTIE 
                 GATGATCGAGTTGGAAGTGTTCCCTTCCGATA 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 CTATCGAGAATGTTAAGGCCAAAATCCAAGA 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 TCATGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                 H68M, E18F 
                 KSLEDGRTLSDY 
                 CTTGCTTTTCAAGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                 LR 
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM72 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [505; 722] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, N25V 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM73 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [506; 723] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 EVKAKIQDHEGIP 
                 ACTATCGAGGAGGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PDQQRLAFQGKS 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, N25E 
                 LEDGRTLSDYNIL 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM74 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [507; 724] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PDQQRLAFQGKS 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, V26I 
                 LEDGRTLSDYNIL 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM75 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [508; 725] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKICDHEGIP 
                 ACTATCGAGAATGTTAAGGCCAAAATCTGCG 
               
               
                   
                 S65P, L67K, 
                 PDQQRLAFQGKS 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, Q31C 
                 LEDGRTLSDYNIL 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM76 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [509; 726] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIWDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCTGGG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, Q31W 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM77 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [510; 727] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIFDHEGIP 
                 ACTATCGAGAATGTTAAGGCCAAAATCTTCG 
               
               
                   
                 S65P, L67K, 
                 PDQQRLAFQGKS 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, Q31F 
                 LEDGRTLSDYNIL 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM78 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [511; 728] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQAHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 CCCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, D32A 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM79 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [512; 729] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33S, A46Q, 
                 NVKAKIQDSEGIP 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PDQQRLAFQGKS 
                 ATTCTGAAGGGATTCCTCCAGATCAACAACGC 
               
               
                   
                 H68M 
                 LEDGRTLSDYNIL 
                 CTTGCTTTTCAAGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 KDPKKMPLLRLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM80 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [513; 730] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K330, A46Q, 
                 NVKAKIQDQEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCAAGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM81 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [514; 731] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33A, A46Q, 
                 NVKAKIQDAEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATGCCGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM82 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [515; 732] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDQQRLAFQGK 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, P38L 
                 SLEDGRTLSDYNI 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM83 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [516; 733] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PCDQQRLAFQG 
                 ATCATGAAGGGATTCCTTGCGATCAACAACG 
               
               
                   
                 H68M, P38C 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM84 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [517; 734] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDEQRLAFQGK 
                 ATCATGAAGGGATTCCTCCAGATGAGCAACG 
               
               
                   
                 H68M, Q40E 
                 SLEDGRTLSDYNI 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM87 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [518; 735] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQHLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACAT 
               
               
                   
                 H68M, R42H 
                 KSLEDGRTLSDY 
                 CTTGCTTTTCAAGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                 LR 
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM88 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [519; 736] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQFLAFQGK 
                 ATCATGAAGGGATTCCTCCAGATCAACAATTC 
               
               
                   
                 H68M, R42F 
                 SLEDGRTLSDYNI 
                 CTTGCTTTTCAAGGGAAGAGCCTGGAGGACG 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 GTCGCACACTGTCTGACTATAACATTCTTAAA 
               
               
                   
                   
                 R 
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM89 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [520; 737] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLTFQGK 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, A44T 
                 SLEDGRTLSDYNI 
                 CCTTACTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM90 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [521; 738] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQGT 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, K48T 
                 SLEDGRTLSDYNI 
                 CCTTGCTTTTCAAGGGACTAGCCTGGAGGAC 
               
               
                   
                   
                 LKDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM92 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [522; 739] 
                 T12M,T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, S49L 
                 KLLEDGRTLSDYN 
                 CCTTGCTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                   
                 ILKDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM93 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [523; 740] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, S49M 
                 KMLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGATGCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM94 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [524; 741] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, E51D 
                 KSLDDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGATGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM95 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [525; 742] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, R54Y 
                 KSLEDGYTLSDYN 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 ILKDPKKMPLLRL 
                 GGTTATACACTGTCTGACTATAACATTClTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM98 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [526; 743] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, S57G 
                 KSLEDGRTLGDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGGGGGACTATAACATTCTTA 
               
               
                   
                   
                 LR 
                 AAGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM101 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [527; 744] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, I61L 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NLLKDPKKMPLL 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAA 
               
               
                   
                   
                 RLR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM102 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [528; 745] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, K63I 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILIDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAT 
               
               
                   
                   
                 LR 
                 TGATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM103 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [529; 746] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65H, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDHKKMPLL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 RLR 
                 AGATCATAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM104 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [530; 747] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, L73M 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 MR 
                 AGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM105 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [531; 748] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, R74Q 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 LQ 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CAA 
               
               
                   
               
               
                 CM107 
                 T7M, T12M, 
                 MLIFVKMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [532; 749] 
                 T14E, K33H, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 A46Q, S65P, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 L67K, H68M, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 P69L, L70V 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                   
                 NILKDPKKMLVL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 RLR 
                 AGATCCTAAAAAGATGTTGGTACTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM108 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [533; 750] 
                 T12M, T14E, 
                 MIELEVYPSDTIE 
                 GATGATCGAGTTGGAAGTGTATCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDQQRLAFQGK 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, E18Y, 
                 LLEDGRTLGDYNI 
                 CCTTGCTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 P38L, S49L, 
                 LKDPKKMPLLRL 
                 GGTCGCACACTGGGGGACTATAACATTCTTA 
               
               
                   
                 S57G 
                 R 
                 AAGATCCTAAAAAGATGCCACTGCTGCGCTT 
               
               
                   
                   
                   
                 GCGT 
               
               
                   
               
               
                 CM110 
                 K6R, T7M, 
                 HLIFVRMLTGKM 
                 CATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [534; 751] 
                 T12M, T14E, 
                 IELEVEPSDTIENV 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPP 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 DQQRLAFQGKSL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, M1H, 
                 EDGRTLSDYNILK 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                 R74Q 
                 DPKKMPLLRLQ 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CAA 
               
               
                   
               
               
                 CM111 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [535; 752] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPPD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLAFQGKSLE 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, M1Y, 
                 DGRTLSDYNILKD 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                 V26I, L73M 
                 PKKMPLLRMR 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM112 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [536; 753] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDEQRLAFQGK 
                 ATCATGAAGGGATTCCTCCAGATGAGCAACG 
               
               
                   
                 H68M, N25V, 
                 SLDDGRTLSDYNI 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGATGAC 
               
               
                   
                 Q40E, E51D 
                 LKDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM113 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [537; 754] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 KSLEDGRTLSDY 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                 K63I 
                 NLLIDPKKMPLLR 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                   
                 LR 
                 TGATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM114 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [538; 755] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQGT 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, E18M, 
                 SLDDGRTLGDYN 
                 CCTTGCTTTTCAAGGGACTAGCCTGGATGACG 
               
               
                   
                 K48T, E51D, 
                 ILKDPKKMPLLRL 
                 GTCGCACACTGGGGGACTATAACATTCTTAA 
               
               
                   
                 S57G 
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM115 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [539; 756] 
                 T12M, T14E, 
                 MIELMVEPSDTIE 
                 GATGATCGAGTTGATGGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDEQRLAFQGK 
                 ATCATGAAGGGATTCCTCCAGATGAGCAACG 
               
               
                   
                 H68M, E16M, 
                 LLEDGRTLSDYNI 
                 CCTTGCTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 N25V, Q40E, 
                 LKDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACATTCTTAA 
               
               
                   
                 S49L 
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM116 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [540; 757] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PDQYRLAFQGKL 
                 ATCATGAAGGGATTCCTCCAGATCAATATCGC 
               
               
                   
                 H68M, V26I, 
                 LEDGRTLGDYNIL 
                 CTTGCTTTTCAAGGGAAGTTGCTGGAGGACG 
               
               
                   
                 Q41Y, S49L, 
                 KDPKKMPLLRLR 
                 GTCGCACACTGGGGGACTATAACATTCTTAA 
               
               
                   
                 S57G 
                   
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM117 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [541; 758] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKICDHEGIP 
                 ACTATCGAGAATGTTAAGGCCAAAATCTGCG 
               
               
                   
                 S65P, L67K, 
                 PDQQHLAFQGK 
                 ATCATGAAGGGATTCCTCCAGATCAACAACAT 
               
               
                   
                 H68M, Q31C, 
                 SLEDGRTLGDYNI 
                 CTTGCTTTTCAAGGGAAGAGCCTGGAGGACG 
               
               
                   
                 R42H,S57G 
                 LKDPKKMPLLRL 
                 GTCGCACACTGGGGGACTATAACATTCTTAA 
               
               
                   
                   
                 R 
                 AGATCCTAAAAAGATGCCACTGCTGCGCTTG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM118 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [542; 759] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 WKAKIFDHEGIP 
                 ACTATCGAGGTAGTTAAGGCCAAAATCTTCG 
               
               
                   
                 S65P, L67K, 
                 PDQQHLAFQGT 
                 ATCATGAAGGGATTCCTCCAGATCAACAACAT 
               
               
                   
                 H68M, E18M, 
                 SLEDGYTLGDYNI 
                 CTTGCTTTTCAAGGGACTAGCCTGGAGGACG 
               
               
                   
                 N25V, Q31F, 
                 LKDPKKMPLLRL 
                 GTTATACACTGGGGGACTATAACATTCTTAAA 
               
               
                   
                 R42H, K48T, 
                 R 
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 R54Y, S57G 
                   
                 GT 
               
               
                   
               
               
                 CM119 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [543; 760] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 WKAKIFDHEGIP 
                 ACTATCGAGGTAGTTAAGGCCAAAATCTTCG 
               
               
                   
                 S65P, L67K, 
                 PDQQHLTFQGTL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACAT 
               
               
                   
                 H68M, E18M, 
                 LEDGYTLGDYNIL 
                 CTTACTTTTCAAGGGACTTTGCTGGAGGACG 
               
               
                   
                 N25V, Q31F, 
                 KDPKKMPLLRLR 
                 GTTATACACTGGGGGACTATAACATTCTTAAA 
               
               
                   
                 R42H, A44T, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 K48T, S49L, 
                   
                 GT 
               
               
                   
                 R54Y, S57G 
                   
                   
               
               
                   
               
               
                 CM120 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [544; 761] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 WKAKIFDHEGIP 
                 ACTATCGAGGTAGTTAAGGCCAAAATCTTCG 
               
               
                   
                 S65P, L67K, 
                 LDQQHLAFQGTS 
                 ATCATGAAGGGATTCCTTTGGATCAACAACAT 
               
               
                   
                 H68M, E18M, 
                 LEDGYTLGDYNIL 
                 CTTGCTTTTCAAGGGACTAGCCTGGAGGACG 
               
               
                   
                 N25V, Q31F, 
                 KDPKKMPLLRLR 
                 GTTATACACTGGGGGACTATAACATTCTTAAA 
               
               
                   
                 P38L, R42H, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 K48T, R54Y, 
                   
                 GT 
               
               
                   
                 S57G 
                   
                   
               
               
                   
               
               
                 CM121 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [545; 762] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQHLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACAT 
               
               
                   
                 H68M, E18M, 
                 TSLEDGYTLGDY 
                 CTTGCTTTTCAAGGGACTAGCCTGGAGGACG 
               
               
                   
                 N25V, R42H, 
                 NILKDPKKMPLLR 
                 GTTATACACTGGGGGACTATAACATTCTTAAA 
               
               
                   
                 K48T, R54Y, 
                 LR 
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 S57G 
                   
                 GT 
               
               
                   
               
               
                 CM131 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [546; 763] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPPD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLAFQGKSLE 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGAGGAC 
               
               
                   
                 K63I, M1Y, 
                 PKKMPLLRMR 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 V26I, L73M 
                   
                 TGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                   
                   
                 CGT 
               
               
                   
               
               
                 CM132 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [547; 764] 
                 T12M, T14E, 
                 ELEVEPSDTIEVIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPPDE 
                 ACTATCGAGGTAATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QRLAFQGKSLDD 
                 ATCATGAAGGGATTCCTCCAGATGAGCAACG 
               
               
                   
                 H68M, I61L, 
                 GRTLSDYNLLIDP 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGATGAC 
               
               
                   
                 K63I, M1Y, 
                 KKMPLLRMR 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 V26I, L73M, 
                   
                 TGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                 N25V, Q40E, 
                   
                 CGT 
               
               
                   
                 E51D 
                   
                   
               
               
                   
               
               
                 CM133 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [548; 765] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDEQRLAFQGK 
                 ATCATGAAGGGATTCCTCCAGATGAGCAACG 
               
               
                   
                 H68M, I61L, 
                 SLDDGRTLSDYN 
                 CCTTGCTTTTCAAGGGAAGAGCCTGGATGAC 
               
               
                   
                 K63I, N25V, 
                 LLIDPKKMPLLRL 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 Q40E, E51D 
                 R 
                 TGATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM134 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [549; 766] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLAFQGT 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 SLDDGRTLGDYN 
                 CCTTGCTTTTCAAGGGACTAGCCTGGATGACG 
               
               
                   
                 K63I, E18M, 
                 LLIDPKKMPLLRL 
                 GTCGCACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 K48T, E51D, 
                 R 
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 S57G 
                   
                 GT 
               
               
                   
               
               
                 CM135 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [550; 767] 
                 T12M, T14E, 
                 MIELMVEPSDTIE 
                 GATGATCGAGTTGATGGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDEQRLAFQGK 
                 ATCATGAAGGGATTCCTCCAGATGAGCAACG 
               
               
                   
                 H68M, I61L, 
                 LLEDGRTLSDYNL 
                 CCTTGCTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, E16M, 
                 LIDPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 N25V, Q40E, 
                   
                 TGATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 S49L 
                   
                 GT 
               
               
                   
               
               
                 CM136 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [551; 768] 
                 T12M, T14E, 
                 ELEVMPSDTIENI 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 DQQRLAFQGTSL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DDGRTLGDYNLLI 
                 CCTTGCTTTTCAAGGGACTAGCCTGGATGACG 
               
               
                   
                 K63I, E18M, 
                 DPKKMPLLRMR 
                 GTCGCACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 K48T, E51D, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCATGC 
               
               
                   
                 S57G, M1Y, 
                   
                 GT 
               
               
                   
                 V26I, L73M 
                   
                   
               
               
                   
               
               
                 CM137 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [552; 769] 
                 T12M, T14E, 
                 ELEVMPSDTIENI 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 DQQRLAFQGTSL 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DDGRTLGDYNLLI 
                 CCTTGCTTTTCAAGGGACTAGCCTGGATGACG 
               
               
                   
                 K63I, E18M, 
                 DPKKMPLLRMQ 
                 GTCGCACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 K48T, E51D, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCATGC 
               
               
                   
                 S57G, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 R74Q 
                   
                   
               
               
                   
               
               
                 CM138 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [553; 770] 
                 T12M, T14E, 
                 ELEVMPSDTIEVI 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPP 
                 ACTATCGAGGTAATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 DEQRLAFQGTSL 
                 ATCATGAAGGGATTCCTCCAGATGAGCAACG 
               
               
                   
                 H68M, I61L, 
                 DDGRTLGDYNLLI 
                 CCTTGCTTTTCAAGGGACTAGCCTGGATGACG 
               
               
                   
                 K63I, E18M, 
                 DPKKMPLLRMQ 
                 GTCGCACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 K48T, E51D, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCATGC 
               
               
                   
                 S57G, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D 
                   
                   
               
               
                   
               
               
                 CM139 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [554; 771] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQRLTFQGK 
                 ATCATGAAGGGATTCCTCCAGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LLEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 LIDPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L 
                   
                 TGATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM 140 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [555; 772] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDQQRLTFQGK 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LLEDGRTLSDYNL 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 LIDPKKMPLLRLR 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L 
                   
                 TGATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                   
                   
                 GT 
               
               
                   
               
               
                 CM141 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [556; 773] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NVKAKIQDHEGI 
                 ACTATCGAGAATGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDQQRLTFQGK 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LLEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 LIDPKKMPLLRLQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 R74Q 
                   
                 AA 
               
               
                   
               
               
                 CM142 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [557; 774] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMPLLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGCCACTGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M 
                   
                   
               
               
                   
               
               
                 CM143 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [558; 775] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDEQRLTFQGK 
                 ATCATGAAGGGATTCCTTTGGATGAGCAACG 
               
               
                   
                 H68M, I61L, 
                 LLDDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGATGACG 
               
               
                   
                 K63I, A44T, 
                 LIDPKKMPLLRLQ 
                 GTCGCACACTGTCTGACTATAACTTGCTTATT 
               
               
                   
                 S49L, P38L, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 R74Q, N25V, 
                   
                 AA 
               
               
                   
                 Q40E, E51D 
                   
                   
               
               
                   
               
               
                 CM144 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [559; 776] 
                 T12M, T14E, 
                 ELEVEPSDTIEVIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLDE 
                 ACTATCGAGGTAATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QRLTFQGKLLDD 
                 ATCATGAAGGGATTCCTTTGGATGAGCAACG 
               
               
                   
                 H68M, I61L, 
                 GRTLSDYNLLIDP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGATGACG 
               
               
                   
                 K63I, A44T, 
                 KKMPLLRMQ 
                 GTCGCACACTGTCTGACTATAACTTGCTTATT 
               
               
                   
                 S49L, P38L, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCATGC 
               
               
                   
                 R74Q, N25V, 
                   
                 AA 
               
               
                   
                 Q40E, E51D, 
                   
                   
               
               
                   
                 M1Y, V26I, 
                   
                   
               
               
                   
                 L73M 
                   
                   
               
               
                   
               
               
                 CM145 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [560; 777] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQHLTFQGT 
                 ATCATGAAGGGATTCCTCCAGATCAACAACAT 
               
               
                   
                 H68M, I61L, 
                 LLEDGYTLGDYNL 
                 CTTACTTTTCAAGGGACTTTGCTGGAGGACG 
               
               
                   
                 K63I, E18M, 
                 LIDPKKMPLLRLR 
                 GTTATACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 N25V, R42H, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 K48T, R54Y, 
                   
                 GT 
               
               
                   
                 S57G, A44T, 
                   
                   
               
               
                   
                 S49L 
                   
                   
               
               
                   
               
               
                 CM146 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [561; 778] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDQQHLTFQGT 
                 ATCATGAAGGGATTCCTTTGGATCAACAACAT 
               
               
                   
                 H68M, I61L, 
                 LLEDGYTLGDYNL 
                 CTTACTTTTCAAGGGACTTTGCTGGAGGACG 
               
               
                   
                 K63I, E18M, 
                 LIDPKKMPLLRLR 
                 GTTATACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 N25V, R42H, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 K48T, R54Y, 
                   
                 GT 
               
               
                   
                 S57G, A44T, 
                   
                   
               
               
                   
                 S49L, P38L 
                   
                   
               
               
                   
               
               
                 CM147 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [562; 779] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDQQHLTFQGT 
                 ATCATGAAGGGATTCCTTTGGATCAACAACAT 
               
               
                   
                 H68M, I61L, 
                 LLEDGYTLGDYNL 
                 CTTACTTTTCAAGGGACTTTGCTGGAGGACG 
               
               
                   
                 K63I, E18M, 
                 LIDPKKMPLLRLQ 
                 GTTATACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 N25V, R42H, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 K48T, R54Y, 
                   
                 AA 
               
               
                   
                 S57G, A44T, 
                   
                   
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q 
                   
                   
               
               
                   
               
               
                 CM148 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [563; 780] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDEQHLTFQGT 
                 ATCATGAAGGGATTCCTTTGGATGAGCAACA 
               
               
                   
                 H68M, I61L, 
                 LLDDGYTLGDYN 
                 TCTTACTTTTCAAGGGACTTTGCTGGATGACG 
               
               
                   
                 K63I, E18M, 
                 LLIDPKKMPLLRL 
                 GTTATACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 N25V, R42H, 
                 Q 
                 GATCCTAAAAAGATGCCACTGCTGCGCTTGC 
               
               
                   
                 K48T, R54Y, 
                   
                 AA 
               
               
                   
                 S57G, A44T, 
                   
                   
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D 
                   
                   
               
               
                   
               
               
                 CM149 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [564; 781] 
                 T12M, T14E, 
                 ELEVMPSDTIEVI 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGGTAATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 EQHLTFQGTLLD 
                 ATCATGAAGGGATTCCTTTGGATGAGCAACA 
               
               
                   
                 H68M, I61L, 
                 DGYTLGDYNLLID 
                 TCTTACTTTTLAAGGGACTTTGCTGGATGALG 
               
               
                   
                 K63I, E18M, 
                 PKKMPLLRMQ 
                 GTTATACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 N25V, R42H, 
                   
                 GATCCTAAAAAGATGCCACTGCTGCGCATGC 
               
               
                   
                 K48T, R54Y, 
                   
                 AA 
               
               
                   
                 S57G, A44T, 
                   
                   
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D, 
                   
                   
               
               
                   
                 M1Y, V26I, 
                   
                   
               
               
                   
                 L73M 
                   
                   
               
               
                   
               
               
                 CM199 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [565; 782] 
                 T12M, T14E, 
                 ELEVMPSDTIEVI 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPP 
                 ACTATCGAGGTAATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 DEQRLAFQGTSL 
                 ATCATGAAGGGATTCCTCCAGATGAGCAACG 
               
               
                   
                 H68M, I61L, 
                 DDGRTLGDYNLLI 
                 CCTTGCTTTTCAAGGGACTAGCCTGGATGACG 
               
               
                   
                 K63I, E18M, 
                 DPKKMLVLRMQ 
                 GTCGCACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 K48T, E51D, 
                   
                 GATCCTAAAAAGATGTTGGTACTGCGCATGC 
               
               
                   
                 S57G, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D, 
                   
                   
               
               
                   
                 P69L, L70V 
                   
                   
               
               
                   
               
               
                 CM203 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [566; 783] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70V 
                   
                   
               
               
                   
               
               
                 CM204 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [567; 784] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDEQRLTFQGK 
                 ATCATGAAGGGATTCCTTTGGATGAGCAACG 
               
               
                   
                 H68M, I61L, 
                 LLDDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGATGACG 
               
               
                   
                 K63I, A44T, 
                 LIDPKKMLVLRLQ 
                 GTCGCACACTGTCTGACTATAACTTGCTTATT 
               
               
                   
                 S49L, P38L, 
                   
                 GATCCTAAAAAGATGTTGGTACTGCGCTTGC 
               
               
                   
                 R74Q, N25V, 
                   
                 AA 
               
               
                   
                 Q40E, E51D, 
                   
                   
               
               
                   
                 P69L, L70V 
                   
                   
               
               
                   
               
               
                 CM208 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [568; 785] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PLDQQHLTFQGT 
                 ATCATGAAGGGATTCCTTTGGATCAACAACAT 
               
               
                   
                 H68M, I61L, 
                 LLEDGYTLGDYNL 
                 CTTACTTTTCAAGGGACTTTGCTGGAGGACG 
               
               
                   
                 K63I, E18M, 
                 LIDPKKMLVLRLQ 
                 GTTATACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 N25V, R42H, 
                   
                 GATCCTAAAAAGATGTTGGTACTGCGCTTGC 
               
               
                   
                 K48T, R54Y, 
                   
                 AA 
               
               
                   
                 S57G, A44T, 
                   
                   
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q, P69L, 
                   
                   
               
               
                   
                 L70V 
                   
                   
               
               
                   
               
               
                 CM210 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [569; 786] 
                 T12M, T14E, 
                 ELEVMPSDTIEVI 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGGTAATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 EQHLTFQGTLLD 
                 ATCATGAAGGGATTCCTTTGGATGAGCAACA 
               
               
                   
                 H68M, I61L, 
                 DGYTLGDYNLLID 
                 TCTTACTTTTLAAGGGACTTTGCTGGATGALG 
               
               
                   
                 N25V, R42H, 
                 PKKMLVLRMQ 
                 GTTATACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 K48T, R54Y, 
                   
                 GATCCTAAAAAGATGTTGGTACTGCGCATGC 
               
               
                   
                 S57G, A44T, 
                   
                 AA 
               
               
                   
                 S49L, P38L, 
                   
                   
               
               
                   
                 R74Q, N25V, 
                   
                   
               
               
                   
                 Q40E, E51D, 
                   
                   
               
               
                   
                 M1Y, V26I, 
                   
                   
               
               
                   
                 L73M, P69L, 
                   
                   
               
               
                   
                 L70V 
                   
                   
               
               
                   
               
               
                 CM211 
                 K6R, T7M, 
                 MLIFVRMLTGK 
                 ATGTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [570; 787] 
                 T12M, T14E, 
                 MIELEVMPSDTIE 
                 GATGATCGAGTTGGAAGTGATGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 VVKAKIQDHEGI 
                 ACTATCGAGGTAGTTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 PPDQQHLAFQG 
                 ATCATGAAGGGATTCCTCCAGATCAACAACAT 
               
               
                   
                 H68M, I61L, 
                 TSLEDGYTLGDY 
                 CTTGCTTTTCAAGGGACTAGCCTGGAGGACG 
               
               
                   
                 K63I, E18M, 
                 NLLIDPKKMLVLR 
                 GTTATACACTGGGGGACTATAACTTGCTTATT 
               
               
                   
                 N25V, R42H, 
                 LR 
                 GATCCTAAAAAGATGTTGGTACTGCGCTTGC 
               
               
                   
                 K48T, R54Y, 
                   
                 GT 
               
               
                   
                 S57G, P69L, 
                   
                   
               
               
                   
                 L70V 
                   
                   
               
               
                   
               
               
                 CM358 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [571; 788] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V 
                   
                   
               
               
                   
               
               
                 CM359 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [572; 789] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMRVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGCGCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69R, L70V 
                   
                   
               
               
                   
               
               
                 CM360 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [573; 790] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMNVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGAATGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69N, L70V 
                   
                   
               
               
                   
               
               
                 CM361 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [574; 791] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMDVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGGATGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69D, L70V 
                   
                   
               
               
                   
               
               
                 CM362 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [575; 792] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMCVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTGCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69C, L70V 
                   
                   
               
               
                   
               
               
                 CM363 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [576; 793] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMEVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGGAGGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69E, L70V 
                   
                   
               
               
                   
               
               
                 CM364 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [577; 794] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMQVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGCAAGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P690, L70V 
                   
                   
               
               
                   
               
               
                 CM365 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [578; 795] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMGVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGGGGGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69G, L70V 
                   
                   
               
               
                   
               
               
                 CM366 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [579; 796] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMHVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGCATGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69H, L70V 
                   
                   
               
               
                   
               
               
                 CM367 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [580; 797] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMIVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGATTGTACTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69I, L70V 
                   
                   
               
               
                   
               
               
                 CM368 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [581; 798] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMKVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGAAGGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69K, L70V 
                   
                   
               
               
                   
               
               
                 CM369 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [582; 799] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 Hb8M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMMVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGATGGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69M, L70V 
                   
                   
               
               
                   
               
               
                 CM370 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [583; 800] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 Hb8M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMFVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTCGTACTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69F, L70V 
                   
                   
               
               
                   
               
               
                 CM371 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [584; 801] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMSVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTCTGTACTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69S, L70V 
                   
                   
               
               
                   
               
               
                 CM372 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [585; 802] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMTVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGACTGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69T, L70V 
                   
                   
               
               
                   
               
               
                 CM373 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [586; 803] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMWVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTGGGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69W, L70V 
                   
                   
               
               
                   
               
               
                 CM374 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [587; 804] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMYVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTATGTACTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69Y, L70V 
                   
                   
               
               
                   
               
               
                 CM375 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [588; 805] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMVVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGGTAGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69V, L70V 
                   
                   
               
               
                   
               
               
                 CM376 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [589; 806] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLALRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGGCCCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70A 
                   
                   
               
               
                   
               
               
                 CM377 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [590; 807] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLRLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGCGCCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70R 
                   
                   
               
               
                   
               
               
                 CM378 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [591; 808] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLNLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGAATCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70N 
                   
                   
               
               
                   
               
               
                 CM379 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [592; 809] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLDLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGGATCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70D 
                   
                   
               
               
                   
               
               
                 CM380 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [593; 810] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLCLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGTGCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70C 
                   
                   
               
               
                   
               
               
                 CM381 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [594; 811] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLELRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGGAGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70E 
                   
                   
               
               
                   
               
               
                 CM382 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [595; 812] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLQLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGCAACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L700 
                   
                   
               
               
                   
               
               
                 CM383 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [596; 813] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLGLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGGGGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70G 
                   
                   
               
               
                   
               
               
                 CM384 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [597; 814] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLHLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGCATCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70H 
                   
                   
               
               
                   
               
               
                 CM385 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [598; 815] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLILRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGATTCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70I 
                   
                   
               
               
                   
               
               
                 CM386 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [599; 816] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLKLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGAAGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70K 
                   
                   
               
               
                   
               
               
                 CM387 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [600; 817] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLMLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGATGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70M 
                   
                   
               
               
                   
               
               
                 CM388 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [601; 818] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLFLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGTTCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70F 
                   
                   
               
               
                   
               
               
                 CM389 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [602; 819] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLPLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGCCTCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70P 
                   
                   
               
               
                   
               
               
                 CM390 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [603; 820] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLSLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGTCTCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70S 
                   
                   
               
               
                   
               
               
                 CM391 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [604; 821] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLTLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGACTCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70T 
                   
                   
               
               
                   
               
               
                 CM392 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [605; 822] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLWLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGTGGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70W 
                   
                   
               
               
                   
               
               
                 CM393 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [606; 823] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLLID 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PKKMLYLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCTTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TGATCCTAAAAAGATGTTGTATCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70Y 
                   
                   
               
               
                   
               
               
                 CM429 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [607; 824] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM430 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [608; 825] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMGVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGGGGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69G, L70V, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM431 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [609; 826] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMLMLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGTTGATGCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69L, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM432 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [610; 827] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAMLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCATGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM433 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [611; 828] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAFLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCTTCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM434 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [612; 829] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMACLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCTGCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM435 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [613; 830] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMGMLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGGGATGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69G, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM436 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [614; 831] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMGFLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGGGTTCCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69G, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM437 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [615; 832] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMGCLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGGGTGCCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69G, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM438 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [616; 833] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMCMLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGTGCATGCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69C, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM439 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [617; 834] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMMMLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGATGATGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69M, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM440 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [618; 835] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMFMLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGTTCATGCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69F, L70M, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM441 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [619; 836] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 AISPEKMAVLRM 
                 GGTCGCACACTGTCTGACTATAACTTGGCCAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62A, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM442 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [620; 837] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLR 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCGCAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62R, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM443 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [621; 838] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 NISPEKMAVLRM 
                 GGTCGCACACTGTCTGACTATAACTTGAATAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62N, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM444 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [622; 839] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 DISPEKMAVLRM 
                 GGTCGCACACTGTCTGACTATAACTTGGATAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62D, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM445 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [623; 840] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLC 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGTGCAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62C, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM446 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [624; 841] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLE 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGGAGAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62E, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM447 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [625; 842] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 QISPEKMAVLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCAAAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L620, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM448 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [626; 843] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 GISPEKMAVLRM 
                 GGTCGCACACTGTCTGACTATAACTTGGGGA 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62G, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM449 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [627; 844] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 HISPEKMAVLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCATAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62H, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM450 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [628; 845] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLII 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 SPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGATTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62I, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM451 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [629; 846] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLK 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGAAGAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62K, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM452 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [630; 847] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 MISPEKMAVLR 
                 GGTCGCACACTGTCTGACTATAACTTGATGAT 
               
               
                   
                 S49L, P38L, 
                 MO 
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62M, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM453 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [631; 848] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLF 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGTTCAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62F, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM454 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [632; 849] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLS 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGTCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62S, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM455 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [633; 850] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLT 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM456 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [634; 851] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 WISPEKMAVLR 
                 GGTCGCACACTGTCTGACTATAACTTGTGGAT 
               
               
                   
                 S49L, P38L, 
                 MQ 
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62W, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM457 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [635; 852] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLY 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGTATAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62Y, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM458 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [636; 853] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 VISPEKMAVLRM 
                 GGTCGCACACTGTCTGACTATAACTTGGTAAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2M, L62V, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM459 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [637; 854] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMCFLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGTGCTTCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69C, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM460 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [638; 855] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMMFLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGATGTTCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69M, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM461 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [639; 856] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMFFLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATCilTCTTCCTGCGCATaC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69F, L70F, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM462 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [640; 857] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMCCLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGTGCTGCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69C, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM463 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [641; 858] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMMCLRM 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGATGTGCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69M, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM464 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [642; 859] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68N, I61L, 
                 LEDGRTLSDYNLP 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMFCLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGCCTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGTTCTGCCTGCGCATGC 
               
               
                   
                 R74Q, M1Y, 
                   
                 AA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69F, L70C, 
                   
                   
               
               
                   
                 L2M, L62P, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM465 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [643; 860] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 AISPEKMAMLR 
                 GGTCGCACACTGTCTGACTATAACTTGGCCAT 
               
               
                   
                 S49L, P38L, 
                 MQ 
                 TTCTCCTGAGAAGATGGCCATGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62A, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM467 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [644; 861] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLC 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAMLRM 
                 GGTCGCACACTGTCTGACTATAACTTGTGCAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCATGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62C, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM468 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [645; 862] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLT 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAMLRM 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                 Q 
                 TTCTCCTGAGAAGATGGCCATGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM469 
                 K6R, T7M, 
                 YMIFVRMLTGK 
                 TATATGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [646; 863] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNL 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 VISPEKMAMLR 
                 GGTCGCACACTGTCTGACTATAACTTGGTAAT 
               
               
                   
                 S49L, P38L, 
                 MQ 
                 TTCTCCTGAGAAGATGGCCATGCTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70M, 
                   
                   
               
               
                   
                 L2M, L62V, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM478 
                 K6R, T7M, 
                 YAIFVRMLTGKM 
                 TATGCCATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [647; 864] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2A, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM479 
                 K6R, T7M, 
                 YRIFVRMLTGKM 
                 TATCGCATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [648; 865] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2R, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM480 
                 K6R, T7M, 
                 YNIFVRMLTGKM 
                 TATAATATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [649; 866] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2N, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM481 
                 K6R, T7M, 
                 YDIFVRMLTGKM 
                 TATGATATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [650; 867] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2D, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM482 
                 K6R, T7M, 
                 YCIFVRMLTGKM 
                 TATTGCATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [651; 868] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2C, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM483 
                 K6R, T7M, 
                 YEIFVRMLTGKM 
                 TATGAGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [652; 869] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2E, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM484 
                 K6R, T7M, 
                 YQIFVRMLTGKM 
                 TATCAAATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [653; 870] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2Q, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM485 
                 K6R, T7M, 
                 YGIFVRMLTGKM 
                 TATGGGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [654; 871] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2G, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM486 
                 K6R, T7M, 
                 YHIFVRMLTGKM 
                 TATCATATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [655; 872] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2H, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM487 
                 K6R, T7M, 
                 YIIFVRMLTGKMI 
                 TATATTATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [656; 873] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2I, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM488 
                 K6R, T7M, 
                 YKIFVRMLTGKM 
                 TATAAGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [657; 874] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2K, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM489 
                 K6R, T7M, 
                 YLIFVRMLTGKMI 
                 TATTTGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [658; 875] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CLTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L62T, D64S, 
                   
                   
               
               
                   
                 K66E 
                   
                   
               
               
                   
               
               
                 CM490 
                 K6R, T7M, 
                 YFIFVRMLTGKMI 
                 TATTTCATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [659; 876] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2F, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM491 
                 K6R, T7M, 
                 YSIFVRMLTGKMI 
                 TATTCTATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [660; 877] 
                 T12M, T14E, 
                 ELEVEPSDTIENIK 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 AKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2S, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM492 
                 K6R, T7M, 
                 YTIFVRMLTGKM 
                 TATACTATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [661; 878] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2T, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM493 
                 K6R, T7M, 
                 YWIFVRMLTGK 
                 TATTGGATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [662; 879] 
                 T12M, T14E, 
                 MIELEVEPSDTIE 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 NIKAKIQDHEGIP 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 LDQQRLTFQGKL 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 LEDGRTLSDYNLT 
                 CCTTACTTTTLAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 ISPEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2W, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM494 
                 K6R, T7M, 
                 YYIFVRMLTGKM 
                 TATTATATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [663; 880] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2Y, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM495 
                 K6R, T7M, 
                 YVIFVRMLTGKM 
                 TATGTAATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [664; 881] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2V, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
                   
                   
               
               
                   
               
               
                 CM496 
                 K6R, T7M, 
                 YPIFVRMLTGKM 
                 TATCCTATTTTCGTACGCATGTTGACTGGAAA 
               
               
                 [665; 882] 
                 T12M, T14E, 
                 IELEVEPSDTIENI 
                 GATGATCGAGTTGGAAGTGGAGCCTTCCGAT 
               
               
                   
                 K33H, A46Q, 
                 KAKIQDHEGIPLD 
                 ACTATCGAGAATATTAAGGCCAAAATCCAAG 
               
               
                   
                 S65P, L67K, 
                 QQRLTFQGKLLE 
                 ATCATGAAGGGATTCCTTTGGATCAACAACG 
               
               
                   
                 H68M, I61L, 
                 DGRTLSDYNLTIS 
                 CCTTACTTTTCAAGGGAAGTTGCTGGAGGAC 
               
               
                   
                 K63I, A44T, 
                 PEKMAVLRMQ 
                 GGTCGCACACTGTCTGACTATAACTTGACTAT 
               
               
                   
                 S49L, P38L, 
                   
                 TTCTCCTGAGAAGATGGCCGTACTGCGCATG 
               
               
                   
                 R74Q, M1Y, 
                   
                 CAA 
               
               
                   
                 V26I, L73M, 
                   
                   
               
               
                   
                 P69A, L70V, 
                   
                   
               
               
                   
                 L2P, L62T, 
                   
                   
               
               
                   
                 D64S, K66E 
               
               
                   
               
               
                   a The SEQ ID NOS shown in brackets correspond to the protein amino acid SEQ ID NO, followed by the DNA nucleic acid SEQ ID NO. 
               
            
           
         
       
     
     Definitions 
     To aid in understanding the invention, several terms are defined below. 
     The use of the terms “a” and “an” and “the” and similar referents in the context of describing the invention (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms “comprising,” “having,” “including,” and “containing” are to be construed as open-ended terms (i.e., meaning “including, but not limited to,”) unless otherwise noted. Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the invention. 
     The term “CRISPR” refers to Clustered Regularly Interspaced Short Palindromic Repeat bacterial adaptive immune system. 
     The terms “Cas” and “Cas endonuclease” generally refers to a CRISPR-associated endonuclease. 
     The term “Cas protein” generally refers to a wild-type protein, including a variant thereof, of a CRISPR-associated endonuclease (including the interchangeable terms Cas and Cas endonuclease). 
     The term “Cas nucleic acid” generally refers to a nucleic acid of a CRISPR-associated endonuclease, including a guide RNA, sgRNA, crRNA, or tracrRNA. 
     The terms “Cas9” and “CRISPR/Cas9” refer to the CRISPR-associated bacterial adaptive immune system of  Steptococcus pyogenes . Examples of this system are disclosed in U.S. patent application Ser. Nos. 15/729,491 and 15/964,041, filed Oct. 10, 2017 and Apr. 26, 2018, respectively (Attorney Docket Nos. IDT01-009-US and IDT01-009-US-CIP, respectively), the contents of which are incorporated by reference herein. 
     The terms “AsCas12a” and “CRISPR/AsCas12a” refer to the CRISPR-associated bacterial adaptive immune system of  Acidaminococcus  sp. Examples of this system are disclosed in U.S. patent application Ser. No. 16/536,256, filed Aug. 8, 2019, (Attorney Docket No. IDT01-013-US), the contents of which are incorporated by reference herein. 
     The terms “LbCas12a” and “CRISPR/LbCas12a” refer to the CRISPR-associated bacterial adaptive immune system of Lachnospiraceae bacterium. Examples of this system are disclosed in U.S. Patent Application Ser. No. 63/018,592, filed May 1, 2020, (Attorney Docket No. IDT01-017-PRO), the contents of which are incorporated by reference herein. 
     The term “variant,” as that term modifies a protein (for example, ubiquitin), refers to a protein that includes at least one amino substitution of the reference, typically wild-type, protein amino acid sequence, additional amino acids (for example, such as an affinity tag or nuclear localization signal), or a combination thereof. 
     The term “polypeptide” refers to any linear or branched peptide comprising more than one amino acid. Polypeptide includes protein or fragment thereof or fusion thereof, provided such protein, fragment or fusion retains a useful biochemical or biological activity. In terms or manufacturing methods, “polypeptide” refers to synthetic polypeptides that may be produced from chemical means as well as polypeptides expressed from translation in vitro or in vivo. 
     The terms “fusion protein” and “fusion polypeptide” are interchangeable and typically includes extra amino acid information that is not native to the protein to which the extra amino acid information is covalently attached. Such extra amino acid information may include tags that enable purification or identification of the fusion protein. Such extra amino acid information may include peptides that enable the fusion proteins to be transported into cells and/or transported to specific locations within cells. Examples of tags for these purposes include affinity tags and nuclear localization signals (NLS), such as those obtained from SV40, allow for proteins to be transported to the nucleus immediately upon entering the cell. Given that the native Cas9 protein is bacterial in origin and therefore does not naturally comprise a NLS motif, addition of one or more NLS motifs to the recombinant Cas9 protein is expected to show improved genome editing activity when used in eukaryotic cells where the target genomic DNA substrate resides in the nucleus. One skilled in the art would appreciate these various fusion tag technologies, as well as how to make and use fusion proteins that include them 
     The terms “Ubiquitin” or “human Ubiquitin” refers to the wild-type Ubiquitin polypeptide amino acid sequence (SEQ ID NO:1). 
     The terms “i53,” i53 Ubiquitin,” or “Ubiquitin i53” refers to a ubiquitin variant polypeptide amino acid sequence (SEQ ID NO:2) that lacks the carboxy terminal di-glycine of the wild-type Ubiquitin polypeptide and includes several amino acid substitutions (Q2L, I44A, Q49S, Q62L, E64D, T66K, L69P, and V70L) relative to the wild-type Ubiquitin polypeptide. 
     The terms “polynucleotide” and “nucleic acid” are interchangeable and refer to synthetic DNA or synthetic RNA, including synthetic mRNA, as well as RNA, including mRNA that may be expressed from DNA or from a vector in vitro or in vivo. The SEQ ID NOS of polynucleotides have been presented in DNA forms without limiting that the corresponding RNA versions, including mRNA versions of those sequences may be readily deduces by one skilled in the art. Accordingly, while the SEQ ID NOS of polynucleotides formally define DNA sequences, such SEQ ID NOS implicitly encompass the RNA sequence counterparts of those DNA sequences as well. 
     One of ordinary skill in the art would appreciate that an isolated polypeptide or isolated polynucleotide comprising a particular SEQ ID NO will encompass the particular amino acid or nucleotide sequence defined by the SEQ ID NO as well as include any additional amino acid or nucleotide information not included within the given SEQ ID NO. 
     REFERENCES 
     
         
         1. Chapman, J. R., M. R. Taylor, and S. J. Boulton,  Playing the endgame: DNA double - strand break repair pathway choice . Mol Cell, 2012. 47(4): p. 497-510. 
         2. Iwabuchi, K., et al.,  Two cellular proteins that bind to wild - type but not mutant p 53. Proc Natl Acad Sci USA, 1994. 91(13): p. 6098-102. 
         3. Escribano-Diaz, C., et al.,  A cell cycle - dependent regulatory circuit composed of  53 BP 1- RIF 1  and BRCA 1- CtIP controls DNA repair pathway choice . Mol Cell, 2013. 49(5): p. 872-83. 
         4. Feng, L., et al.,  RIF 1  counteracts BRCA 1- mediated end resection during DNA repair . J Biol Chem, 2013. 288(16): p. 11135-43. 
         5. Xie, A., et al.,  Distinct roles of chromatin - associated proteins MDC 1  and  53 BP 1  in mammalian double - strand break repair . Mol Cell, 2007. 28(6): p. 1045-57. 
         6. Gaj, T., et al.,  Genome - Editing Technologies: Principles and Applications . Cold Spring Harb Perspect Biol, 2016. 8(12). 
         7. Botuyan, M. V., et al.,  Structural basis for the methylation state - specific recognition of histone H 4- K 20  by  53 BP 1  and Crb 2  in DNA repair . Cell, 2006. 127(7): p. 1361-73. 
         8. Charier, G., et al.,  The Tudor tandem of  53 BP: a new structural motif involved in DNA and RG - rich peptide binding . Structure, 2004. 12(9): p. 1551-62. 
         9. Fradet-Turcotte, A., et al., 53 BP 1  is a reader of the DNA - damage - induced H 2 A Lys  15  ubiquitin mark . Nature, 2013. 499(7456): p. 50-4. 
         10. Mattiroli, F., et al.,  RNF 168  ubiquitinates K 13-15  on H 2 A H 2 AX to drive DNA damage signaling . Cell, 2012. 150(6): p. 1182-95. 
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     All references, including publications, patent applications, and patents, cited herein are hereby incorporated by reference to the same extent as if each reference were individually and specifically indicated to be incorporated by reference and were set forth in its entirety herein. 
     Preferred embodiments of this invention are described herein, including the best mode known to the inventors for carrying out the invention. Variations of those preferred embodiments may become apparent to those of ordinary skill in the art upon reading the foregoing description. 
     The inventors expect skilled artisans to employ such variations as appropriate, and the inventors intend for the invention to be practiced otherwise than as specifically described herein. Accordingly, this invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law. Moreover, any combination of the above-described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context.