Patent Publication Number: US-2017369492-A1

Title: 1,3-substituted 2-amino-indole derivatives and analogues useful in the treatment or prevention of diabetes mellitus, obesity, and inflammatory bowel disease

Description:
TECHNICAL FIELD 
     The present invention relates to 1,3-substituted 2-amino-indole derivatives and analogues, processes for their preparation, pharmaceutical compositions containing them and their use in therapy, particularly in the treatment or prevention of conditions having an association with the GPR43 receptor, such as diabetes mellitus, obesity and inflammatory bowel disease. 
     BACKGROUND OF THE INVENTION 
     Targeting the release of anorectic and antidiabetic gut peptides is the focus of many ongoing drug development programs, as evidence is accumulating that enhanced secretion of Peptide YY (PYY) and Glucagon-Like Peptide-1 (GLP-1) from intestinal L-cells may translate into beneficial effects in subjects with diabetes and obesity. 
     Short chain fatty acids (SCFA), derived from bacterial fermentation of macrofibrous material reaching the distal gut are known to reach high concentrations under physiological conditions in the colons of healthy subjects. Non-digestible and fermentable dietary fibre, as well as SCFA themselves, have been shown to increase GLP-1 and PYY secretion in humans (Zhou et al., Am. J. Physiol. Endocrinol. Metab., 2008, vol. 295(5), pp. E1160-E1166), and enhanced PYY release has been proposed as a link between luminal SCFA and altered gut motility (Dumoulin et al., Endocrinology, 1998, vol. 139(9), pp. 3780-3786). 
     SCFA act as a local nutrient source, but can also trigger cell-specific signalling cascades by activation of the G-protein coupled free fatty acid receptors, GPR41 (FFAR 3 ) and GPR43 (FFAR 2 ) (Brown et al., J. Biol. Chem., 2003, vol. 278(13), pp. 11312-11319). The finding that both receptors are located in colonic L cells by immunostaining (Tazoe et al., Biomed. Res., 2009, vol. 30(3), pp. 149-156), suggests that short chain fatty acids may utilise this pathway to modulate L-cell function. In addition to L cells, GPR43 is also expressed in Islets of Langerhans, white adipose tissue, bone marrow and spleen. 
     GPR43 knockout mice have impaired glucose tolerance, with reduced insulin secretion and reduced GLP-1 secretion (Tolhurst et al., Diabetes, 2012, vol. 61, pp. 364-371). They have increased fat mass and a mild increase in food intake. From this it can be deduced that activation of the GPR43 receptor should lead to beneficial effects in the treatment of diabetes and obesity. 
     GPR43 is also expressed on a variety of immune cells, so may represent a potential treatment for certain inflammatory diseases and conditions (Bindels L B, Dewulf E M, Delzenne N M., Trends Pharmacol Sci., 2013, 34(4), Pp. 226-32; Macia L et al., Nat Commun, 2015, 6, article 6734; and Smith, P M et al, Science, 2013, 341 (6145), pp. 569-573). 
     There is therefore a need for compounds that activate the GPR43 receptor. 
     Certain 3-substituted 2-amino-indole analogues are known in the art. WO 2004/060893 describes a broad class of such compounds useful for treating a variety of diseases modulated by potassium channels. Other substituted indole analogues are known from WO 2012/064897, WO 2005/023818, WO 2011/140164, WO 2011/153553 and US 2014/0018361. 
     SUMMARY OF THE INVENTION 
     In accordance with the present invention, there is provided a compound of formula (I): 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein
         Q represents —O—, —S—, —SO—, —SO 2 —, —SO 2 NR—, —SO 2 (CH 2 ) m — or —SO 2 O—;   R represents a hydrogen atom or a C 1 -C 6  alkyl group;   m is 1 or 2;   X 4  represents N or CR 4 ;   X 5  represents N or CR 5 ;   X 6  represents N or CR 6 ;   X 7  represents N or CR 7 ;
 
provided that one or two of X 4 , X 5 , X 6  and X 7  represents a nitrogen atom;
   R 1  and R 2  each independently represent a hydrogen atom or a C 1 -C 6  alkyl, C 3 -C 8  cycloalkyl or C 1 -C 6  alkoxycarbonyl group, each of which may be optionally substituted by at least one halogen atom;   R 3  represents a saturated or unsaturated 3- to 10-membered ring system which may comprise at least one ring heteroatom independently selected from nitrogen, oxygen and sulphur, wherein the 3- to 10-membered ring system is optionally substituted by at least one substituent independently selected from halogen, hydroxyl, cyano, C 1 -C 6  alkyl, C 1 -C 6  haloalkyl, C 1 -C 6  hydroxyalkyl, C 1 -C 6  alkoxy, C 1 -C 6  haloalkoxy, C 3 -C 6  cycloalkylC 1 -C 6  alkoxy, C 1 -C 6  alkoxyC 1 -C 6  alkyl, C 1 -C 6  alkylC(O)NR 14 —, phenyl, (halo)phenylcarbonyl, phenoxy, benzyl, benzyloxycarbonyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group, which heterocyclyl group is itself optionally substituted by at least one C 1 -C 6  alkyl group,   and when Q represents —SO 2 NR—, R 3  may additionally represent a C 1 -C 6  alkyl group optionally substituted by at least one substituent independently selected from halogen, C 1 -C 6  alkoxy, C 3 -C 6  cycloalkyl, phenyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group;   R 4 , R 5  and R 6  each independently represent a hydrogen or a halogen atom, or a C 1 -C 6  alkyl, C 1 -C 6  alkoxy, C 1 -C 6  alkylthio, C 1 -C 6  haloalkyl, NR 12 R 13 , C 3 -C 8  cycloalkyl or C 5 -C 8  cycloalkenyl group;   R 7  represents a hydrogen or a halogen atom, hydroxyl, cyano, NR 9 R 10 , or a C 1 -C 6  alkyl, C 3 -C 8  cycloalkyl, C 2 -C 6  alkenyl, C 5 -C 8  cycloalkenyl, C 1 -C 6  alkoxy, C 3 -C 8  cycloalkyloxy, benzyloxy, 3- to 11-membered saturated heterocyclyl, 3- to 11-membered saturated heterocyclyloxy, C 6 -C 10  aryl or heteroaryl group, each of which may be optionally substituted by at least one substituent independently selected from halogen, cyano, C 1 -C 6  alkyl, C 1 -C 6  alkoxy, C 3 -C 8  cycloalkyl, phenyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group wherein each C 1 -C 6  alkyl, C 1 -C 6  alkoxy, C 3 -C 8  cycloalkyl, phenyl or saturated or unsaturated 4- to 6-membered heterocyclyl substituent group may itself be optionally substituted by at least one substituent independently selected from halogen, C 1 -C 3  alkyl, C 1 -C 3  alkoxy and C 3 -C 6  cycloalkyl;   either R 8  represents a saturated 3- to 8-membered ring system which may comprise at least one ring heteroatom independently selected from nitrogen, oxygen and sulphur, wherein the 3- to 8-membered ring system is optionally substituted by at least one substituent independently selected from halogen, hydroxyl and C 1 -C 6  alkyl, or R 8  represents a C 1 -C 6  alkyl group optionally substituted by at least one substituent independently selected from phenyl and C 3 -C 6  cycloalkyl, the cycloalkyl group itself being optionally substituted by at least one C 1 -C 6  alkyl group;   R 9  and R 10  each independently represent a hydrogen atom, or a C 1 -C 6  alkyl or —(CH 2 ) p —R 11  group, each of which may be optionally substituted by at least one substituent independently selected from halogen, C 1 -C 3  alkyl and C 1 -C 3  alkoxy;   p is 0 or 1;   R 11  represents C 3 -C 6  cycloalkyl, phenyl or a saturated or unsaturated 5- to 6-membered heterocyclyl group; and   R 12 , R 13  and R 14  each independently represent a hydrogen atom or a C 1 -C 6  alkyl group.       

     In the context of the present specification, unless otherwise stated, an “alkyl” substituent group or an alkyl moiety in a substituent group may be linear or branched. Examples of alkyl groups/moieties include methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, t-butyl, n-pentyl, and n-hexyl. 
     A “haloalkyl” substituent group or a haloalkyl moiety in a substituent group refers to an alkyl group or moiety in which one or more, e.g. one, two, three, four or five, hydrogen atoms are replaced independently by halogen atoms, i.e. by fluorine, chlorine, bromine or iodine atoms. Examples of haloalkyl groups/moieties include fluoromethyl, difluoromethyl, trifluoromethyl, 2,2-difluoroethyl and 2,2,2-trifluoroethyl. 
     A “hydroxyalkyl” substituent group or a hydroxyalkyl moiety in a substituent group refers to an alkyl group or moiety in which one or more, e.g. one, two, three, four or five, hydrogen atoms are replaced by hydroxyl groups, examples of which include —CH 2 OH, —CH 2 CH 2 OH, —CH 2 CH 2 CH 2 OH, —CH(OH)CH 2 OH, —CH(CH 3 )OH and —CH(CH 2 OH) 2 . 
     The term “(halo)phenylcarbonyl” denotes a phenylcarbonyl group which is optionally substituted with from 1 to 5 independently selected halogen atoms, an example of which is fluorophenylcarbonyl. 
     A “cycloalkyl” substituent group or a cycloalkyl moiety in a substituent group refers to a saturated hydrocarbyl ring containing, for example, from 3 to 8 carbon atoms, examples of which include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. Unless stated otherwise, a cycloalkyl substituent group or moiety may include monocyclic, bicyclic (e.g. fused or spiro) and polycyclic hydrocarbyl rings. 
     An “alkenyl” substituent group or an alkenyl moiety in a substituent group refers to an unsaturated alkyl group or moiety having one or more carbon-carbon double bonds. Examples of alkenyl groups/moieties include ethenyl, propenyl, 1-butenyl, 2-butenyl, 1-pentenyl, 1-hexenyl, 1,3-butadienyl, 1,3-pentadienyl, 1,4-pentadienyl and 1,4-hexadienyl. 
     A “cycloalkenyl” substituent group or a cycloalkenyl moiety in a substituent group refers to an unsaturated hydrocarbyl ring having one or more carbon-carbon double bonds and containing, for example, from 3 to 8 carbon atoms, examples of which include cyclopent-1-en-1-yl, cyclohex-1-en-1-yl and cyclohex-1,3-dien-1-yl. Unless stated otherwise, a cycloalkenyl substituent group or moiety may include monocyclic, bicyclic (e.g. fused or spiro) and polycyclic hydrocarbyl rings. 
     A “C 6 -C 10  aryl” group refers to a group derived from an aromatic hydrocarbon containing from six to ten carbon atoms. The aryl group may be monocyclic or polycyclic (e.g. bicyclic) in which the two or more rings are fused, examples of which include phenyl, 1-naphthyl and 2-naphthyl. Also included within the scope of the term “aryl”, as it is used herein, is a group in which an aromatic ring is fused to one or more non-aromatic rings as exemplified by indanyl and tetrahydronaphthyl. An aryl group may be bonded at any suitable ring atom. 
     A “heteroaryl” group is a 5- to 10-membered aryl group in which from 1 to 4 ring carbon atoms are replaced by heteroatoms independently selected from nitrogen, oxygen and sulphur. The heteroaryl group can be bonded at any suitable ring atom (i.e. at any carbon or heteroatom of the heteroaryl ring system). Examples of heteroaryl groups include the following: 
     
       
         
         
             
             
         
       
         
         
           
             G=O, S or NH 
           
         
       
    
     The term “halogen” includes fluorine, chlorine, bromine and iodine. 
     When a group or moiety is described as being ‘unsaturated’, it should be understood that the group or moiety may be partially or fully unsaturated and thus may have aliphatic or aromatic properties. 
     For the purposes of the present invention, where a combination of moieties is referred to as one group, for example, arylalky or alkoxycarbonyl, the last mentioned moiety contains the atom by which the group is attached to the rest of the molecule. An example of an arylalkyl group is benzyl and an example of an alkoxycarbonyl group is —C(O)OCH 3 . 
     It will be appreciated that the invention does not encompass any unstable structures or any divalent —O—O—, —O—S— or —S—S— moieties. When any chemical moiety or group is described as being optionally substituted, it will be appreciated that the moiety or group may be either unsubstituted or substituted by one or more of the specified substituents. It will be appreciated that the number and nature of substituents will be selected so as to avoid sterically undesirable combinations. 
     In an embodiment of the invention, one of X 4 , X 5 , X 6  and X 7  is N, e.g. X 4  is N or X 7  is N. 
     In another embodiment of the invention, two of X 4 , X 5 , X 6  and X 7  are N, e.g. 
     X 4  and X 7  are N, X 5  is CR 5  and X 6  is CR 6 , or
 
X 5  and X 7  are N, X 4  is CR 4  and X 6  is CR 6 , or
 
X 4  and X 6  are N, X 5  is CR 5  and X 7  is CR 7 , or
 
X 6  and X 7  are N, X 4  is CR 4  and X 5  is CR 5 .
 
     In a particular embodiment, X 4  and X 7  are N, X 5  is CR 5  and X 6  is CR 6 . 
     As stated above, Q represents —O—, —S—, —SO—, —SO 2 —, —SO 2 NR—, —SO 2 (CH 2 ) m — or —SO 2 O—. When Q represents an SO 2 NR—, —SO 2 (CH 2 ) m — or —SO 2 O— group, the group will be attached to the central ring system through the sulphur atom. 
     In one embodiment of the invention, Q represents —SO 2 — or —SO 2 NR—. 
     R represents a hydrogen atom or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl group. In one embodiment, R represents a hydrogen atom or a methyl group. 
     In a further embodiment, Q represents —SO 2 —. 
     As stated above, R 1  and R 2  each independently represent a hydrogen atom or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl, C 3 -, C 4 -, C 5 - or C 6 -C 8  cycloalkyl or C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxycarbonyl group, each of which may be optionally substituted by at least one halogen atom, e.g. one, two, three or four halogen atoms independently selected from fluorine and chlorine atoms. 
     In one embodiment, R 1  and R 2  each independently represent a hydrogen atom or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl, C 3 -C 6  cycloalkyl or C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxycarbonyl group, each of which may be optionally substituted by one or two halogen atoms independently selected from fluorine and chlorine atoms. 
     In another embodiment, R 1  and R 2  each independently represent a hydrogen atom. 
     In a further embodiment, one of R 1  and R 2  represents a hydrogen atom and the other of R 1  and R 2  represents a C 1 -C 2  alkyl (such as methyl), C 3 -C 6  cycloalkyl (such as cyclohexyl) or C 1 -C 2  alkoxycarbonyl (such as methoxycarbonyl) group, each of which may be optionally substituted by one or two fluorine atoms. 
     Examples of R 1  and R 2  substituents include hydrogen atoms and methyl, 4,4-difluorocyclohexyl and methoxycarbonyl groups. 
     As stated above, R 5  represents a saturated or unsaturated 3- to 10-membered (e.g. 3-, 4-, 5- or 6- to 7-, 8-, 9- or 10-membered) ring system which may comprise at least one ring heteroatom (e.g. one, two, three or four ring heteroatoms) independently selected from nitrogen, oxygen and sulphur, wherein the 3- to 10-membered ring system is optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), hydroxyl, cyano, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2 , alkyl, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  haloalkyl, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  hydroxyalkyl, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxy, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  haloalkoxy, C 3 -C 6  cycloalkylC 1 -C 6  alkoxy (e.g. cyclopropylC 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxy, specifically cyclopropylmethoxy), C 1 -C 6  alkoxyC 1 -C 6  alkyl (e.g. C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxymethyl, specifically methoxymethyl), C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkylC(O)NR 14 , phenyl, (halo)phenylcarbonyl, phenoxy, benzyl, benzyloxycarbonyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group, which heterocyclyl group is itself optionally substituted by at least one C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl group, 
     and when Q represents —SO 2 NR—, R 3  may additionally represent a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl group optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxy, C 1 -C 6  cycloalkyl, phenyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group. 
     This R 3  saturated or unsaturated 3- to 10-membered ring system may comprise one or more (e.g. one, two, three or four) ring heteroatoms independently selected from nitrogen, oxygen and sulphur. The ring system may be monocyclic or polycyclic (e.g. bicyclic) in which the two or more rings are fused, bridged or spiro. If the ring system is unsaturated, it may be partially or fully unsaturated. The ring system can be bonded to Q at any suitable ring atom (i.e. at any carbon or heteroatom of the ring system). 
     Examples of R 5  saturated or unsaturated 3- to 10-membered ring systems include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclopentenyl, cyclohexenyl, bicyclo[2.2.1]heptyl, azabicyclo[3.2.1]octanyl, phenyl, azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, oxadiazolyl (e.g. 1,2,4-oxadiazolyl), tetrahydrofuranyl, naphthyl, benzofuranyl, benzothienyl, benzodioxolyl, 2,3-dihydro-1,4-benzodioxinyl, benzoxazolyl, quinolinyl, isoquinolinyl, 1,2,3,4-tetrahydroquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl, oxazolyl, thiadiazolyl (e.g. 1,2,3-thiadiazolyl), 2,3-dihydroindenyl, 1,4-oxazepanyl, azepanyl, 2,3-dihydrobenzofuranyl, 2,3-dihydroisoindolyl, tetrahydropyranyl, 2,3-dihydro-1H-pyrrolo[3,4-c]pyridinyl, pyrazolyl, imidazo[1,2-a]pyridinyl, pyrazinyl, thiazolidinyl, indanyl, thienyl, isoxazolyl, pyridazinyl, pyrrolyl, furanyl, thiazolyl, isothiazolyl, indolyl, isoindolyl, imidazolyl, pyrimidinyl, benzimidazolyl, triazolyl, tetrazolyl and pyridinyl. 
     In one aspect, the R 3  saturated or unsaturated 3- to 10-membered ring system is selected from phenyl, thienyl, cyclopropyl, cyclohexyl, pyridinyl, pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, azetidinyl, 1,4-oxazepanyl, azepanyl, thiomorpholinyl, 1,2,3,4-tetrahydroisoquinolinyl, 2,3-dihydroisoindolyl, azabicyclo[3.2.1]octanyl and 2,3-dihydro-1,4-benzodioxinyl. 
     If present in R 3 , a saturated or unsaturated 4- to 6-membered heterocyclyl substituent group contains from 1 to 4 ring heteroatoms independently selected from nitrogen, oxygen and sulphur, examples of which include azetidinyl, oxetanyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, tetrahydrofuranyl, tetrahydropyranyl, oxadiazolyl, pyrrolyl, imidazolyl, pyridinyl, pyrazinyl, pyridazinyl, pyrimidinyl, thienyl and furanyl. 
     In one embodiment of the invention, R 5  represents a saturated or 3-, 4-, 5- or 6-membered ring system which may comprise at least one ring heteroatom (e.g. one, two, three or four ring heteroatoms) independently selected from nitrogen, oxygen and sulphur, wherein the 3-, 4-, 5- or 6-membered ring system is optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), hydroxyl, cyano, C 1 -C 2  alkyl, C 1 -C 2  haloalkyl, C 1 -C 2  hydroxyalkyl, C 1 -C 2  alkoxy, C 1 -C 2  haloalkoxy, C 3 -C 6  cycloalkylC 1 -C 2  alkoxy, C 1 -C 2  alkoxyC 1 -C 2  alkyl, C 1 -C 2  alkylC(O)NR 14 —, phenyl, (halo)phenylcarbonyl, phenoxy, benzyl, benzyloxycarbonyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group, which heterocyclyl group is itself optionally substituted by at least one, e.g. one or two, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl groups which may be the same or different to one another, 
     and when Q represents —SO 2 NR—, R 3  may additionally represent a C 1 -C 4  alkyl group optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), C 1 -C 2  alkoxy, C 3 -C 6  cycloalkyl, phenyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group. 
     In another embodiment, R 3  represents a saturated 4- to 6-membered ring system which may comprise one or two ring heteroatoms independently selected from nitrogen, oxygen and sulphur (e.g. cyclohexyl, azetidinyl, pyrrolidinyl, piperidinyl, piperazinyl, thiomorpholinyl or morpholinyl), wherein the saturated 4- to 6-membered ring system is optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), hydroxyl, C 1 -C 2  alkyl, C 1 -C 2  haloalkyl, C 1 -C 2  hydroxyalkyl, C 1 -C 2  alkoxy, C 1 -C 2  haloalkoxy, C 3 -C 6  cycloalkylC 1 -C 2  alkoxy, C 1 -C 2  alkoxyC 1 -C 2  alkyl, C 1 -C 2  alkylC(O)NR 14 —, phenyl, fluorophenylcarbonyl, phenoxy, benzyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group, which heterocyclyl group is itself optionally substituted by at least one C 1 -C 2  alkyl group. 
     In an alternative embodiment, R 3  represents an unsaturated, e.g. aromatic, 6- to 10-membered ring system which may comprise at least one ring heteroatom (e.g. one, two, three or four ring heteroatoms) independently selected from nitrogen, oxygen and sulphur, wherein the unsaturated 6- to 10-membered ring system is optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), cyano, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  haloalkyl, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxy, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  haloalkoxy, benzyloxycarbonyl and a saturated or unsaturated 5- to 6-membered heterocyclyl group, which heterocyclyl group is itself optionally substituted by at least one, e.g. one or two, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl groups which may be the same or different to one another. 
     In a further embodiment, R 3  represents a phenyl or pyridinyl group which is optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine or chlorine), cyano, C 1 -C 2  alkyl, C 1 -C 2  haloalkyl (e.g. trifluoromethyl), C 1 -C 4  alkoxy, C 1 -C 2  haloalkoxy (e.g. difluoromethoxy or trifluoromethoxy), benzyloxycarbonyl and a saturated or unsaturated 5- to 6-membered heterocyclyl group (e.g. morpholinyl), which heterocyclyl group is itself optionally substituted by at least one, e.g. one or two, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl groups which may be the same or different to one another. 
     In a still further embodiment, R 5  represents phenyl optionally substituted by one or two substituents independently selected from fluorine, chlorine, cyano, methyl, trifluoromethyl, difluoromethoxy, trifluoromethoxy and C 1 -C 3  alkoxy. 
     In yet another embodiment, R 3  represents an unsubstituted phenyl group. 
     In still another embodiment, when Q represents —SO 2 NR—, R 3  represents a C 1 -C 4  alkyl group optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), C 1 -C 2  alkoxy, C 3 -C 6  cycloalkyl, phenyl and a saturated or unsaturated 4- to 6-membered heterocyclyl group (e.g. oxetanyl, tetrahydrofuranyl or thiazolyl). 
     In a particular embodiment of the invention, R 3  represents any one of the following moieties or is selected from a group containing any two or more of such moieties:
         (i) 1-N-benzylcarboxylate-piperidin-4-yl,   (ii) 2,3-difluorophenyl,   (iii) 2-fluoro-4-methoxyphenyl,   (iv) 2-fluoro-4-methylphenyl,   (v) 2-fluorophenyl,   (vi) 2-methoxyphenyl,   (vii) 2-methylphenyl,   (viii) 3,4-difluorophenyl,   (ix) 3,5-difluorophenyl,   (x) 3-chloro-4-methoxyphenyl,   (xi) 3-fluoro-4-methoxyphenyl,   (xii) 3-fluorophenyl,   (xiii) 3-methoxyphenyl,   (xiv) 3-methylphenyl,   (xv) 4-(difluoromethoxy)phenyl,   (xvi) 4-(fluoromethoxy)phenyl,   (xvii) 4-(propan-2-yloxy)phenyl,   (xviii) 4-(trifluoromethyl)phenyl,   (xix) 4-bromo-2-[(2S)-2-methylmorpholin-4-yl]-phenyl,   (xx) 4-bromo-2-fluorophenyl,   (xxi) 4-chloro-2-fluorophenyl,   (xii) 4-chloro-3-fluorophenyl,   (xxiii) 4-chlorophenyl,   (xxiv) 4-fluoro-2-methoxyphenyl,   (xxv) 4-fluoro-2-methylphenyl,   (xxvi) 4-fluorophenyl,   (xxvii) 4-methoxyphenyl,   (xxviii) 4-methylphenyl,   (xxix) 4-cyanophenyl,   (xxx) 6-methoxypyridin-3-yl,   (xxxi) tetrahydrofuranylmethyl,   (xxxii) 2-methoxyethyl,   (xxxiii) (1,3-thiazol-2-yl)ethyl,   (xxxiv) propyl,   (xxxv) 3,3,3-trifluoropropyl,   (xxxvi) butyl,   (xxxvii) cyclopropyl,   (xxxviii) cyclopropylmethyl,   (xxxix) cyclobutylmethyl,   (xl) cyclohexyl,   (xli) oxan-4-yl,   (xlii) oxolan-3-yl,   (xliii) phenyl,   (xliv) 2-phenylethyl,   (xlv) pyridin-2-yl,   (xlvi) pyridin-3-yl,   (xlvii) benzyl,   (xlviii) thienyl,   (xlix) azetidinyl,   (l) 3-methoxyazetidin-1-yl,   (li) 3-phenoxyazetidin-1-yl,   (lii) 3-(piperidin-1-yl)azetidin-1-yl,   (liii) 3-(pyrazol-1-yl)azetidin-1-yl,   (liv) pyrrolidinyl,   (lv) 2-methylpyrrolidin-1-yl,   (lvi) 3-methylpyrrolidin-1-yl,   (lvii) 3,3-dimethylpyrrolidin-1-yl,   (lviii) 3-methoxypyrrolidin-1-yl,   (lix) 3-(methoxymethyl)pyrrolidin-1-yl,   (lx) 3-phenylpyrrolidin-1-yl,   (lxi) piperidinyl,   (lxii) 4-hydroxypiperidin-1-yl,   (lxiii) 4-hydroxymethylpiperidin-1-yl,   (lxiv) 3-methylpiperidin-1-yl,   (lxv) 4-methylpiperidin-1-yl,   (lxvi) 3,3-dimethylpiperidin-1-yl,   (lxvii) 4,4-dimethylpiperidin-1-yl,   (lxviii) 4-methoxypiperidin-1-yl,   (lxix) 4-ethoxypiperidin-1-yl,   (lxx) 4,4-difluoropiperidin-1-yl,   (lxxi) 4-(trifluoromethyl)piperidin-1-yl,   (lxxii) 4-(cyclopropylmethoxy)piperidin-1-yl,   (lxxiii) 4-phenylpiperidin-1-yl,   (lxxiv) 4-phenoxypiperidin-1-yl,   (lxxv) 4-benzylpiperidin-1-yl,   (lxxvi) piperazinyl,   (lxxvii) 4-methylpiperazin-1-yl,   (lxxviii) (4-fluorophenylcarbonyl)piperazin-1-yl,   (lxxix) 2,2,2-trifluoroethylpiperazinyl,   (lxxx) morpholinyl,   (lxxxi) 2,6-dimethylmorpholin-4-yl,   (lxxxii) thiomorpholinyl,   (lxxxiii) 1,4-oxazepanyl,   (lxxiv) azepanyl,   (lxxxv) 4-(methylacetamido)piperidin-1-yl,   (lxxxvi) oxetanyl,   (lxxxvii) oxetan-3-ylmethyl,   (lxxxviii) tetrahydroiosquinolinyl,   (lxxxix) 2,3-dihydroisoindol-2-yl,   (xc) azabicyclo[3.2.1]octanyl,   (xci) (hydroxy)azabicyclo[3.2.1]octanyl, and   (xcii) 2,3-dihydro-1,4-benzodioxin-6-yl.       

     If present, R 4 , R 5  and R 6  each independently represent a hydrogen or a halogen atom, or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl (e.g. methyl or ethyl), C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxy (e.g. methoxy), C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkylthio (e.g. methylthio), C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  haloalkyl (e.g. trifluoromethyl), NR 12 R 13  (e.g. dimethylamino), C 3 -C 8  cycloalkyl (e.g. cyclopropyl or cyclohexyl) or C 5 -C 8  cycloalkenyl (e.g. cyclohexenyl) group. 
     In an embodiment of the invention, R 4  represents a hydrogen atom. 
     In an embodiment of the invention, R 5  represents a hydrogen or halogen (e.g. chlorine) atom, or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl (e.g. methyl or ethyl) group. 
     In an embodiment of the invention, R 6  represents a hydrogen atom, or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl (e.g. methyl or ethyl) group. 
     In a further embodiment, R 5  and R 6  each independently represent a hydrogen or chlorine atom or a methyl group. 
     As stated above, R 7  represents a hydrogen or a halogen atom, hydroxyl, cyano, NR 9 R 10 , or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl, C 3 -, C 4 - or C 5 - to C 6 -, C 7 - or C 8 -cycloalkyl, C 2 -C 6  or C 2 -C 4  alkenyl, C 5 -C 8  or C 5 -C 6  cycloalkenyl, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxy, C 3 -, C 4 - or C 5 - to C 6 -, C 7 - or C 8 -cycloalkyloxy, benzyloxy, 3- to 11-membered saturated heterocyclyl, 3- to 11-membered saturated heterocyclyloxy, C 6 -C 10  aryl or heteroaryl group, each of which may be optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen, cyano, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl, C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxy, C 3 -C 8  or C 3 -C 6  cycloalkyl, phenyl and a saturated or unsaturated 5- to 6-membered heterocyclyl group wherein each C 1 -C 6  alkyl, C 1 -C 6  alkoxy, C 3 -C 8  cycloalkyl, phenyl or saturated or unsaturated 5- to 6-membered heterocyclyl substituent group may itself be optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen, C 1 -C 3  alkyl, C 1 -C 3  alkoxy and C 1 -C 6  cycloalkyl. 
     The R 7  3- to 1-membered saturated heterocyclyl group or moiety contains from 1 to 4 ring heteroatoms independently selected from nitrogen, oxygen and sulphur. Furthermore, the group or moiety may be monocyclic or polycyclic (e.g. bicyclic) in which the two or more rings are fused, bridged or spiro. The R 7  saturated heterocyclyl group can be bonded to the central ring system through any suitable ring atom (i.e. through any carbon or heteroatom of the heterocyclyl group). Examples of such 3- to 11-membered saturated heterocyclyl groups or moieties include azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, azepanyl, oxazepanyl, tetrahydrofuranyl, tetrahydropyranyl, 6-azaspiro[2.5]octanyl, 6-oxa-9-azaspiro[4.5]decanyl, 2-oxa-6-azaspiro[3.5]nonanyl, 4-oxa-7-azaspiro[2.5]octanyl, 5-oxa-8-azaspiro[3.5]nonanyl, 8-oxa-3-azabicyclo[3.2.1]octanyl and octahydrocyclopenta[b]morpholinyl. 
     The R 7  heteroaryl group contains from 1 to 4 ring heteroatoms independently selected from nitrogen, oxygen and sulphur. The group may be monocyclic, or bicyclic in which the rings are fused together. Specific examples of R 7  heteroaryl groups include pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, thienyl, furyl, furazanyl, oxazolyl, thiazolyl, oxadiazolyl, isothiazolyl, isoxazolyl, thiadiazolyl, tetrazinyl, quinoxalinyl, benzothiazolyl, benzoxazolyl, quinolinyl, quinazolinyl, indolyl, 7-azaindolyl, indolizinyl, indazolyl, imidazo[1,2-a]pyridinyl and 7H-pyrrolo[2,3-d]pyrimidinyl. If present, the R 7  saturated or unsaturated 5- to 6-membered heterocyclyl substituent group contains from 1 to 4 ring heteroatoms independently selected from nitrogen, oxygen and sulphur, examples of which include pyrrolidinyl, piperidinyl, morpholinyl, piperazinyl, tetrahydrofuranyl, tetrahydropyranyl, dioxolanyl, oxadiazolyl, pyrrolyl, imidazolyl, pyridinyl, pyrazinyl, pyridazinyl, pyrimidinyl, thienyl and furanyl. 
     In an embodiment of the invention, R 7  represents a hydrogen or a halogen atom (e.g. fluorine, chlorine or bromine), hydroxyl, cyano, NR 9 R 10 , or a C 1 -C 4  alkyl, C 3 -C 6  cycloalkyl, C 2 -C 4  alkenyl, C 5 -C 6  cycloalkenyl, C 1 -C 6  alkoxy, C 3 -C 6  cycloalkyloxy, benzyloxy, 3- to 11-membered saturated heterocyclyl, 3- to 6-membered saturated heterocyclyloxy, C 6 -C 10  aryl or 5- to 6-membered heteroaryl group, each of which may be optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen, cyano, C 1 -C 4  alkyl, C 1 -C 4  alkoxy, C 3 -C 6  cycloalkyl, phenyl and a saturated or unsaturated 5- to 6-membered heterocyclyl group wherein each C 1 -C 4  alkyl, C 1 -C 4  alkoxy, C 3 -C 6  cycloalkyl, phenyl or saturated or unsaturated 5- to 6-membered heterocyclyl substituent group may itself be optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine or chlorine), C 1 -C 3  alkyl (e.g. methyl), C 1 -C 3  alkoxy (e.g. methoxy) and C 3 -C 6  cycloalkyl (e.g. cyclopropyl). 
     In a second embodiment, R 7  represents a hydrogen or a halogen atom (e.g. fluorine, chlorine or bromine), hydroxyl, cyano, NR 9 R 10 , or a C 1 -C 4  alkyl, C 3 -C 6  cycloalkyl, C 2 -C 4  alkenyl, C 5 -C 6  cycloalkenyl, C 1 -C 6  alkoxy, C 3 -C 6  cycloalkyloxy, benzyloxy, 3- to 6-membered saturated heterocyclyl (e.g. azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl or thiomorpholinyl), 5- to 6-membered saturated heterocyclyloxy (e.g. tetrahydrofuranyloxy or tetrahydropyranyloxy), phenyl, pyrazolyl or pyridinyl group, each of which may be optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen, cyano, C 1 -C 4  alkyl, C 1 -C 4  alkoxy, C 3 -C 6  cycloalkyl, phenyl and a saturated or unsaturated 5- to 6-membered heterocyclyl group (e.g. tetrahydrofuranyl, tetrahydropyranyl, pyridinyl, pyrazolyl, thiazolyl and oxazolyl), wherein each C 1 -C 4  alkyl, C 1 -C 4  alkoxy, C 3 -C 6  cycloalkyl, phenyl or saturated or unsaturated 5- to 6-membered heterocyclyl substituent group may itself be optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine or chlorine), C 1 -C 3  alkyl (e.g. methyl), C 2 -C 3  alkoxy (e.g. methoxy) and C 3 -C 6  cycloalkyl (e.g. cyclopropyl). 
     If R 7  represents a group NR 9 R 10 , then as stated above R 9  and R 10  each independently represent a hydrogen atom, or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl or —(CH 2 ) p —R 11  group, each of which may be optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine or chlorine), C 1 -C 3  alkyl (e.g. methyl) and C 1 -C 3  alkoxy (e.g. methoxy). 
     As stated above, p is 0 or 1 and R 11  represents C 3 -C 6  cycloalkyl, phenyl or a saturated or unsaturated 5- to 6-membered heterocyclyl group. This R 11  saturated or unsaturated 5-to 6-membered heterocyclyl group is as defined above for R 7 . 
     In one aspect, R 9  and R 10  each independently represent a hydrogen atom, or a C 1 -C 4  alkyl or R 11  group, each of which may be optionally substituted as previously defined. 
     In another aspect, R 9  and R 10  each independently represent a hydrogen atom, or a C 1 -C 4  alkyl or R 11  group selected from cyclopropyl, tetrahydrofuranyl and tetrahydropyranyl, each of which may be optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from fluorine and methyl. 
     In yet another aspect, one of R 9  and R 10  represents a hydrogen atom or a C 1 -C 6  alkyl (e.g. methyl) group and the other of R 9  and R 10  represents a group —(CH 2 )—R 11 , each of which may be optionally substituted as previously defined. 
     In still another aspect, one of R 9  and R 10  represents a hydrogen atom or a methyl group, and the other of R 9  and R 10  represents a —(CH 2 )—R 11  group optionally substituted as previously defined, wherein R 11  is selected from oxazolyl, pyridinyl, dioxolanyl, phenyl, tetrahydrofuranyl, tetrahydropyranyl, cyclohexyl, furanyl, cyclopropyl and pyrazolyl. 
     In a third embodiment, R 7  is represented by a group of formula: 
     
       
         
         
             
             
         
       
     
     wherein
         X A  represents N or CH;   each X B  independently represents a single bond or —C(R 14 ) 2 —, provided that at least one X B  represents —C(R 14 ) 2 —;   each R 14  independently represents a hydrogen or a halogen atom or a cyano, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl or phenyl group;   X C  represents —O—, —S—, —C(R 15 ) 2 — or —NR 15 —;   each R 15  independently represents a hydrogen or a halogen atom or a C 1 -C 4  alkyl or C 1 -C 4  haloalkyl group, or two R 15  groups may together represent a —(C(R 18 ) 2 ) n — group, wherein each R 18  independently represents a hydrogen or a halogen atom and n is 2, 3, 4 or 5;   each R 16  independently represents a hydrogen or a halogen atom or a cyano, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl or phenyl group, or two R 16  may together represent a —(C(R 19 ) 2 ) q — group, wherein each R 16  independently represents a hydrogen or a halogen atom and q is 2, 3, 4 or 5; and   each R 17  independently represents a hydrogen or a halogen atom or a cyano, C 1 -C 4  alkyl, C 1 -C 4  haloalkyl or phenyl group, or two R 17  may together represent a —(C(R 20 ) 2 ) t — group, wherein each R 20  independently represents a hydrogen or a halogen atom and t is 2, 3, 4 or 5.       

     In one embodiment, X A  in formula (A) represents N. 
     In another embodiment, both X B  moieties in formula (A) represent CH 2 . 
     In a further embodiment, in formula (A), one XP represents CH 2  and the other X B  represents CH(CH 3 ), or one X B  represents CH 2  and the other X B  represents a single bond. 
     In one embodiment, X C  in formula (A) represents —O— or —S—. 
     In one embodiment, in formula (A), both R 16  represent a hydrogen atom and at least one R 17  is other than a hydrogen atom, or both R 17  represent a hydrogen atom and at least one R is other than a hydrogen atom. 
     In another embodiment, in formula (A), at least one R 16  is other than a hydrogen atom and at least one R 17  is other than a hydrogen atom. 
     In one embodiment, if present in formula (A), each R 18  represents a hydrogen atom and n is 2. 
     In one embodiment, if present in formula (A), each R 19  represents a hydrogen atom and q is 2, 3 or 4. 
     In one embodiment, if present in formula (A), each R 20  represents a hydrogen atom and t is 2, 3 or 4. 
     In a fourth embodiment, R 7  is represented by a group of formula (A) wherein
         X A  represents N;   each X B  independently represents a single bond or —C(R 14 ) 2 —, provided that at least one X B  represents —C(R 14 ) 2 —;   each R 14  independently represents a hydrogen atom or a methyl group;   X C  represents —O—;   each R 16  independently represents a hydrogen or a halogen (e.g. fluorine) atom or a C 1 -C 4  alkyl, C 1 -C 4  haloalkyl (e.g. trifluoromethyl) or phenyl group, or two R 16  may together represent a —(CH 2 ) q — group, wherein q is 2, 3 or 4; and   each R 17  independently represents a hydrogen or a halogen (e.g. fluorine) atom or a C 1 -C 4  alkyl, C 1 -C 4  haloalkyl (e.g. trifluoromethyl) or phenyl group, or two R 17  may together represent a —(CH 2 ) t — group, wherein t is 2, 3 or 4.       

     In a fifth embodiment, R 7  is represented by a group of formula (A) wherein
         X A  represents N;   each X B  independently represents a single bond or —C(R 14 ) 2 —, provided that at least one X B  represents —C(R 14 ) 2 —;   each R 14  independently represents a hydrogen atom or a methyl group;   X C  represents —O—;   each R 16  independently represents a hydrogen or a fluorine atom or a methyl, trifluoromethyl or phenyl group, or two R 16  may together represent a —(CH) 2 ) q — group, wherein q is 2, 3 or 4; and   each R 17  independently represents a hydrogen or a fluorine atom or a methyl, trifluoromethyl or phenyl group, or two R 17  may together represent a —(CH 2 ) t — group, wherein t is 2, 3 or 4.       

     In a sixth embodiment, R 7  represents a hydrogen or a halogen atom (e.g. fluorine, chlorine or bromine), hydroxyl, cyano, NR 9 R 10  (e.g. methylamino or dimethylamino), or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkoxy or benzyloxy group. 
     In a particular embodiment of the invention, R 7  represents any one of the following moieties or is selected from a group containing any two or more of such moieties: hydrogen, bromine and chlorine atoms and (1-methylcyclopropyl)methoxy, (2,2-difluorocyclopropyl)methoxy, (2,6-dimethyloxan-4-yl)oxy, (2-methylcyclopropyl)methoxy, (2R)-2-(methoxymethyl)pyrrolidin-1-yl, (2R)-2-methylmorpholin-4-yl, (2R)-2-phenylmorpholin-4-yl, (2R,5R)-2,5-dimethylmorpholin-4-yl, (2R,6R)-2,6-dimethylmorpholin-4-yl, (2S)-2-methylmorpholin-4-yl, (2S)-2-phenylmorpholin-4-yl, (2S,5S)-2,5-dimethylmorpholin-4-yl, (3,3-difluorocyclobutyl)methoxy, (3R)-oxolan-3-yloxy, (3S)-oxolan-3-yloxy, (4,4-difluorocyclohexyl)oxy, (4-methyl-1,3-thiazol-2-yl)methoxy, (dimethyl-1,3-oxazol-4-yl)methoxy, (E)-2-cyclopropylethenyl, 1-(pyridin-2-yl)ethoxy, 1,4-oxazepan-4-yl, 1-cyclopentylethoxy, 1-cyclopropylethoxy, 1H-pyrazol-1-yl, 1-phenylethoxy, 2-(2-methylpropy)morpholin-4-yl, 2-(methoxymethyl)morpholin-4-yl, 2-(propan-2-yl)morpholin-4-yl, 2-(trifluoromethyl)morpholin-4-yl, 2,2-diethylmorpholin-4-yl, 2,2-dimethylmorpholin-4-yl, 2,2-dimethylpyrrolidin-1-yl, 2,5-dimethylmorpholin-4-yl, 2,6-dimethylthiomorpholin-4-yl, 2-cyano-morpholin-4-yl, 2-cyclopropylethyl, 2-cyclopropylmorpholin-4-yl, 2-ethyl-2-methylmorpholin-4-yl, 2-ethylmorpholin-4-yl, 2-ethylthiomorpholin-4-yl, 2-methoxyethoxy, 2-methylmorpholin-4-yl, 2-methylphenyl, 2-methylpiperidin-1-yl, 2-methylthiomorpholin-4-yl, 2-oxa-6-azaspiro[3.5]nonan-(6-yl, 3-(1H-pyrazol-1-yl)piperidin-1-yl, 3,3-difluoropiperidin-1-yl, 3,3-difluoropyrrolidin-1-yl, 3,3-dimethylpyrrolidin-1-yl, 3,5-dimethyl-1H-pyrazol-1-yl, 3-ethoxypiperidin-1-yl, 3-methoxypiperidin-1-yl, 3-methoxypyrrolidin-1-yl, 3-methylmorpholin-4-yl, 3-methylphenyl, 3-methylpiperidin-1-yl, 4-(cyclopropylmethoxy)piperidin-1-yl, 4-(methoxymethyl)piperidin-1-yl, 4,4-difluorocyclohex-1-en-1-yl, 4,4-difluorocyclohexyl, 4,4-difluoropiperidin-1-yl, 4-fluoropiperidin-1-yl, 4-methoxypiperidin-1-yl, 4-methylphenyl, 4-methylpiperidin-1-yl, 4-oxa-7-azaspiro[2.5]octan-7-yl, 5-oxa-8-azaspiro[3.5]nonan-8-yl, 6-azaspiro[2.5]octan-6-yl, 6-oxa-9-azaspiro[4.5]decan-9-yl, 8-oxa-3-azabicyclo[3.2.1]octan-3-yl, azepan-1-yl, azetidin-1-yl, benzyloxy, cyclobutoxy, cyclohex-1-en-1-yl, cyclohexyl, cyclohexylmethoxy, cyclohexyloxy, cyclopent-1-en-1-yl, cyclopentyl, cyclopentylmethoxy, cyclopentyloxy, cyclopropylmethoxy, ethylamino, morpholin-4-yl, N-(1,3-dioxolan-2-ylmethyl)-N-methyl-amino, N-(2,2-difluoroethyl)-N-methyl-amino, N-(2,2-dimethyloxan-4-yl)-N-methyl-amino, N-(cyclohexylmethyl)-N-ethylamino, N-(cyclopropylmethyl)-4-N-(oxolan-2 ylmethyl)-amino, N-(cyclopropylmethyl)-amino, N,N-diethylamino, N-[(2-methoxyphenyl)methyl]-N-methyl-amino, N-[(3-chlorophenyl)methyl]-N-methyl-amino, N-cyclopropyl-N-methyl-amino, N-ethyl-4-N-(furan-2-ylmethyl)-amino, N-ethyl-4-N-[(1-methyl-1H-pyrazol-4-yl)methyl]-amino, N-ethyl-N-(oxan-4-ylmethyl)-amino, N-ethyl-N-methyl-amino, N-methyl-4-[(5-methyl-1,2-oxazol-3-yl)methyl]-amino, N-methyl-N-(oxan-2-ylmethyl)-amino, N-methyl-N-(oxan-4-yl)-amino, N-methyl-N-(propan-2-yl)-amino, N-methyl-N-(pyridin-2-ylmethyl)-amino, octahydrocyclopenta[b]morpholin-4-yl, oxan-2-ylmethoxy, oxan-3-ylmethoxy, oxan-4-ylmethoxy, oxan-4-yloxy, oxolan-3-ylmethoxy, pentan-3-yloxy, phenyl, piperidin-1-yl, prop-1-en-2-yl, propan-2-yl, pyridin-3-yl, pyridin-4-yl, pyrrolidin-1-yl, hydroxyl, cyano, methoxy, ethoxy, benzyloxy, N-methylamino and N-dimethylamino. 
     As stated above, either R 8  represents a saturated 3- to 8-membered ring system which may comprise at least one ring heteroatom (e.g. one, two, three or four ring heteroatoms) independently selected from nitrogen, oxygen and sulphur, wherein the 3- to 8-membered ring system is optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), hydroxyl and C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl, or R 8  represents a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl group optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from phenyl and C 3 -C 6  cycloalkyl, the cycloalkyl group itself being optionally substituted by at least one C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl group. 
     This R 8  saturated 3- to 8-membered ring system may comprise one or more (e.g. one, two, three or four) ring heteroatoms independently selected from nitrogen, oxygen and sulphur. The ring system may be monocyclic or bicyclic in which the two or more rings are fused, bridged or spiro, and is attached to the nitrogen atom of the central ring system through a ring carbon atom. Examples of such ring systems include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl, thiomorpholinyl, azepanyl, oxazepanyl and bicyclo[2.2.1]heptanyl. 
     In an embodiment of the invention, R 8  represents a saturated 4- to 7-membered ring system which may comprise at least one ring heteroatom (e.g. one, two, three or four ring heteroatoms) independently selected from nitrogen, oxygen and sulphur, wherein the 4- to 7-membered ring system is optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from halogen (e.g. fluorine, chlorine, bromine or iodine), hydroxyl and C 1 -C 2  alkyl, or R 8  represents a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl group optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from phenyl and C 3 -C 6  cycloalkyl, the cycloalkyl group itself being optionally substituted by at least one (e.g. one or two independently selected) C 1 -C 2  alkyl groups. 
     In one aspect, R 8  represents a C 4 -C 6  cycloalkyl group optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from fluorine, hydroxyl and methyl. 
     In another aspect, R 8  represents a C 1 -C 2  alkyl group optionally substituted by at least one substituent (e.g. one, two, three or four substituents) independently selected from phenyl and C 3 -C 6  cycloalkyl, the cycloalkyl group itself being optionally substituted by one or two independently selected C 1 -C 2  alkyl groups. 
     In a particular embodiment of the invention, R 8  represents any one of the following moieties or is selected from a group containing any two or more of such moieties:
         (i) cyclohexyl,   (ii) cycloheptyl,   (iii)cyclopentyl,   (iv) 4,4-(difluoro)cyclohexyl,   (v) 4-tetrahydropyranyl,   (vi) cyclobutyl,   (vii) (2-methyl)cyclohexyl,   (viii) n-butyl,   (ix) phenethyl,   (x) 2-(hydroxyl)cyclohexyl,   (xi) (cyclopropyl)ethyl,   (xii) (cyclobutyl)ethyl,   (xiii) 3-tetrahydropyranyl,   (xiv) 3,3-(dimethyl)butyl,   (xv) bicyclo[2.2.1]heptanyl,   (xvi) (cyclopentyl)methyl,   (xvii) (ethyl)cyclopropylmethyl, and   (xviii) 2,2-(dimethyl)cyclopropylmethyl.       

     As stated above, R 12  and R 13  each independently represent a hydrogen atom or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl (e.g. methyl) group. 
     In an embodiment of the invention, R 12  and R 13  both represent a methyl group. 
     As stated above, R 14  represents a hydrogen atom or a C 1 -C 6 , or C 1 -C 4 , or C 1 -C 2  alkyl (e.g. methyl) group. 
     In an embodiment of the invention, R 14  represents a methyl group. 
     In an embodiment of the invention, the compound of formula (I) is one in which;
         Q represents —SO 2 —, —SO 2 NH— or —SO 2 N(CH 3 )—;   X 4  represents N;   X 5  represents CR 5 ;   X 6  represents CR 6 ;   X 7  represents N;   R 1  and R 2  each independently represent a hydrogen atom;   R 5  represents a hydrogen or halogen atom, or a C 1 -C 6  alkyl group;   R 6  represents a hydrogen atom or a C 1 -C 6  alkyl group;   R 8  represents a C 4 -C 6  cycloalkyl group optionally substituted by at least one substituent independently selected from fluorine, hydroxyl and methyl; and   R 3  and R 9  to R 13  are as defined above.       

     Examples of compounds of the invention include:
     7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cycloheptyl-7-[(4-methylbenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cycloheptyl-7-[(4-methylbenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclopentyl-7-[(4-methylbenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   7-[(4-chlorobenzene)sulfonyl]-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[(4-fluorobenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-{[4-(propan-2-yloxy)benzene]sulfonyl}-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(thiophene-2-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[3,2-b]pyridin-2-amine,   1-cyclopentyl-3-[(4-methylbenzene)sulfonyl]-1H-pyrrolo[3,2-b]pyridin-2-amine,   1-cyclohexyl-3-[(4-methylbenzene)sulfonyl]-1H-pyrrolo[2,3-b]pyridin-2-amine,   7-(cyclohexanesulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-(4,4-difluorocyclohexyl)-7-[(4-methoxybenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   1-(4,4-difluorocyclohexyl)-3-[(4-methoxybenzene)sulfonyl]-1H-pyrrolo[2,3-b]pyridin-2-amine,   3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-b]pyridin-2-amine,   3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-b]pyridin-2-amine,   3-(benzenesulfonyl)-1-(4,4-difluorocyclohexyl)-1H-pyrrolo[2,3-b]pyridin-2-amine,   7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine,   3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-c]pyridin-2-amine,   3-(benzenesulfonyl)-1-(4,4-difluorocyclohexyl)-1H-pyrrolo[3,2-b]pyridin-2-amine,   1-(4,4-difluorocyclohexyl)-3-[(4-methoxybenzene)sulfonyl]-1H-pyrrolo[3,2b]pyridin-2-amine,   3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[3,2-c]pyridin-2-amine,   methyl N-[7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-yl]carbamate,   3-(benzenesulfonyl)-1-(4,4-difluorocyclohexyl)-6-methyl-1H-pyrrolo[2,3-b]pyridin-2-amine,   7-(benzenesulfonyl)-5-cyclohexyl-4-methoxy-5H-pyrrolo[3,2-d]pyrimidin-6-amine,   5-(benzenesulfonyl)-3-cloro-7-cyclohexyl-7H-pyrrolo[2,3-c]pyridazin-6-amine,   5-(benzenesulfonyl)-7-cyclohexyl-7H-pyrrolo[2,3-c]pyridazin-6-amine,   7-(benzenesulfonyl)-5-(4,4-difluorocyclohexyl)-4-ethoxy-5H-pyrrolo[3,2-d]pyrimidin-6-amine,   7-(benzenesulfonyl)-4-(benzyloxy)-5-(4,4-difluorocyclohexyl)-5H-pyrrolo[3,2d]pyrimidin-6-amine,   6-amino-5-(4,4-difluorocyclohexyl)-7-(phenylsulfonyl)-5H-pyrrolo[3,2-d]pyrimidin-4-ol,   7-(benzenesulfonyl)-4-chloro-5-(4,4-difluorocyclohexyl)-5H-pyrrolo[3,2-d]pyrimidin-6-amine,   7-(benzenesulfonyl)-5-(4,4-difluorocyclohexyl)-4-N-methyl-5H-pyrrolo[3,2-d]pyrimidine-4,6-diamine,   7-(benzenesulfonyl)-5-cyclohexyl-4-N,4-N-dimethyl-5H-pyrrolo[3,2-d]pyrimidine-4,6-diamine,   7-(benzenesulfonyl)-5-cyclohexyl-4-methoxy-5H-pyrrolo[3,2-d]pyrizidin-6-amine,   3-(benzenesulfonyl)-1-cyclohexyl-7-methoxy-1H-pyrrolo[2,3-c]pyridin-2-amine,   6-amino-7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidine-4-carbonitrile,   5-cyclohexyl-7-(2-fluorobenzenesulfonyl)-4-methoxy-2-methyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine,   5-cyclohexyl-7-(3-fluorobenzenesulfonyl)-4-methoxy-2-methyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine,   7-(benzenesulfonyl)-4-methoxy-5-(oxan-4-yl)-5H-pyrrolo[3,2-d]pyrimidin-6-amine,   6-amino-5-cyclohexyl-N-phenyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   6-amino-5-cyclohexyl-N-(pyridin-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   5-cyclobutyl-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-(2-methylcyclohexyl)-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-butyl-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-phenethyl-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   2-(6-amino-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-5-yl)cyclohexanol,   5-(2-cyclopropylethyl)-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-(4,4-difluoro-cyclohexyl)-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-(2-cyclobutylethyl)-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   7-(phenylsulfonyl)-5-(tetrahydro-2H-pyran-3-yl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-(3,3-dimethylbutyl)-7-(phenylsulfonyl-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-(1R*,2R*,4S*)bicyclo[2.2.1]heptan-2-yl)-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-(cyclopentylmethyl)-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-((1-ethylcyclopropyl)-methyl)-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-((2,2-dimethylcyclopropyl)methyl)-7-(phenylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(piperidin-1-ylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(pyrrolidin-1-ylsulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   6-amino-5-cyclohexyl-N-propyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   6-amino-5-cyclohexyl-N-methyl-N-propyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   5-cyclohexyl-7-(morpholinosulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-((4-methylpiperidin-yl)sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-((4-methylpiperazin-1-yl)sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-((3-methoxyazetidin-1-yl)sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-((4-ethoxypiperidin-1-yl)sulfonyl)-5H-pyrrolo[2,3b]pyrazin-6-amine,   5-cyclohexyl-7-((4,4-methylpiperidin-1-yl)sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-((3-methylpyrrolidin-1-yl)sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-((2-methylpyrrolidin-1-yl)sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-((4,4-difluoropiperidin-1-yl)sulfonyl)-5H-pyrrolo[2,3b]pyrazin-6-amine,   6-amino-N-benzyl-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   6-amino-N,5-dicyclohexyl-N-methyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   5-cyclohexyl-7-(1,4-oxazepane-4-sulfonyl)-5H pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(4-methoxypiperidine-1-sulfonyl)-5H pyrrolo[2,3-b]pyrazin-6-amine,   6-amino-N-(cyclobutylmethyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   5-cyclohexyl-7-(3,3-dimethylpyrrolidine-1-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(2,6-dimethylmorpholine-4-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   7-(azepane-1-sulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(thiomorpholine-4-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   N-(1-{6-amino-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl}piperidin-4-yl)-N-methylacetamide,   6-amino-5-cyclohexyl-N-(oxetan-3-ylmethyl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   7-(4-benzylpiperidine-1-sulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine,   6-amino-5-cyclohexyl-N-(3,3,3-trifluoropropyl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   5-cyclohexyl-7-(4-phenylpiperidine-1-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   6-amino-5-cyclohexyl-N-(2-phenylethyl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   5-cyclohexyl-7-(4-phenoxypiperidine-1-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(3-phenylpyrrolidine-1-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[4-(trifluoromethyl)piperidine-1-sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[3-(methoxymethyl)pyrrolidine-1-sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   6-amino-5-cyclohexyl N-(cyclopropylmethyl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   6-amino-5-cyclohexyl-N-(2-methoxyethyl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   5-cyclohexyl-7-(3-methoxypyrrolidine-1-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(3,3-dimethylpiperidine-1-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   1-{6-amino-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl}piperidin-4-ol,   5-cyclohexyl-7-(1,2,3,4-tetrahydroisoquinoline-2-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   6-amino-N-(butan-2-yl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   6-amino-5-cyclohexyl-N-(oxolan-2-ylmethyl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   5-cyclohexoyl-7-(2,3-dihydro-1H-isoindole-2-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-{4-[(4-fluorophenyl)carbonyl]piperazine-1-sulfonyl}-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(3-phenoxyazetidine-1-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[3-(piperidin-1-yl)azetidine-1-sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[3-(1H-pyrazol-1-yl)azetidine-1-sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(3-methylpiperidine-1-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   6-amino-5-cyclohexyl-N-[2-(1,3-thiazol-2-yl)ethyl]-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide,   8-{6-amino-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl}-8-azabicyclo[3.2.1]octan-3-ol,   5-cyclohexyl-7-[4-(2,2,2-trifluoroethyl)-piperazine-1-sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   (1-{6-amino-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl}piperidin-4-yl)methanol,   5-cyclohexyl-7-[4-(cyclopropylmethoxy)piperidine-1-sulfonyl]-5H pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[(4-methoxybenzene)-sulfonyl]-5H pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(cyclopropanesufonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[(3-fluorobenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[(2-fluorobenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-[3-methoxybenzene)-sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine,   4-{6-amino-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl}benzonitrile,   7-[(3-chloro-4-methoxybenzene)-sulfonyl]-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(6-methoxypyridine-3-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-{[4-(trifluoromethoxy)-benzene]sulfonyl}-5H pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-(2,3-dihydro-1,4-benzodioxine-6-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine,   5-cyclohexyl-7-{[4-(difluoromethoxy)-benzene]sulfonyl}-5H-pyrrolo[2,3-b]pyrazin-6-amine,   and pharmaceutically acceptable salts of any one thereof.   

     It should be noted that each of the chemical compounds listed above represents a particular and independent aspect of the invention. 
     The present invention further provides a process for the preparation of a compound of formula (I) or a pharmaceutically acceptable salt thereof as defined above which comprises, 
     (a) when NR 1 R 2  represents NH 2 , reacting a compound of formula 
     
       
         
         
             
             
         
       
     
     wherein L 1  represents a leaving group (e.g. a halogen atom or trifluoromethanesulphonate group) and X 4 , X 5 , X 6 , X 7 , Q and R 3  are as defined in formula (I), with a compound of formula (III), H 2 NR 8 , or a salt thereof (e.g. a hydrochloride salt) wherein R 8  is as defined in formula (I); or
 
(b) when NR 1 R 2  represents NH 2 , reacting a compound of formula
 
     
       
         
         
             
             
         
       
     
     wherein L 2  represents a leaving group (e.g. a halogen atom or trifluoromethanesulphonate group) and X 4 , X 5 , X 6 , X 7  and R 8  are as defined in formula (I), with a compound of formula 
     
       
         
         
             
             
         
       
     
     wherein Q and R 3  are as defined in formula (I);
 
wherein any of compounds (II), (III), (IV) or (V) may optionally be protected;
 
and optionally thereafter carrying out one or more of the following procedures:
         removing any protecting groups   converting a compound of formula (I) into another compound of formula (I)   forming a pharmaceutically acceptable salt.       

     In process (a) the compound of formula (II) may conveniently be combined with an amine of formula (III) or a salt thereof in the presence of a base such as triethylamine or ethylbis(propan-2-yl)amine, in a solvent such as anhydrous N-methylpyrrolidone, to arrive at a compound of formula (I). Typically the reaction mixture is heated, e.g. to around 170° C. under microwave irradiation. 
     Process (b) may conveniently be carried out by combining the compound of formula (IV) with the substituted acetonitrile of formula (V) in the presence of a base such as sodium hydride or sodiobis(trimethylsilyl)amine, and a metal catalyst such as Pd(o), typically where the metal catalyst is in the form of a transition metal complex such as tetrakis(triphenylphosphine) palladium and/or di-tert-butyl[dichloro({di-tert-butyl[4-(dimethylamino)phenyl]-phosphaniumyl})palladio][4-(dimethylamino)phenyl] phosphanium, in a solvent such as 1,2-dimethoxyethane, dioxane or 2-methyloxalane, typically where the solvent is anhydrous, to arrive at a compound of formula (I). Typically the reaction mixture is heated, e.g. to around 70-150° C. under conventional heating or microwave irradiation. Optionally the Pd(o) catalyst may be formed in situ, e.g. from Pd(I) acetate and 2,8,9-tris(2-methylpropyl)-2,5,8,9-tetraaza-1-phosphabicyclo[3.3.3]undecane. 
     Compounds of formula (II) may be prepared by reacting a compound of formula 
     
       
         
         
             
             
         
       
     
     wherein each L 3  independently represents a leaving group (e.g. a halogen atom or trifluoromethanesulphonate group) and X 4 , X 5 , X 6  and X 7  are as defined above, with a compound of formula (V) as defined above. The reaction is conveniently carried out in the presence of a base such as sodium hydride, and a metal catalyst such as Pd(o), typically where the metal catalyst is in the form of a transition metal complex such as tetrakis(triphenylphosphine) palladium, in a solvent such as anhydrous 1,2-dimethoxyethane, to arrive at a compound of formula (II) which may or may not be isolated. Typically the reaction mixture is heated, e.g. to around 70-140° C. under conventional heating or microwave irradiation. 
     In one embodiment, a compound of formula (I) or a salt or a protected form thereof, may be converted into another compound of formula (I) or a salt or a protected form thereof. 
     For example, a compound of formula (I) or a salt or a protected form thereof, where R 1  and R 2  are both hydrogen, may be converted into another compound of formula (I) or a salt or a protected form thereof where one or both of R 1  and R 2  are not hydrogen, typically by treatment with a compound of formula R 1 -L and/or R 2 -L, where R 1  and R 2  are as previously defined but not hydrogen and L is as previously defined for L 1 . 
     In one convenient procedure, a compound of formula (I) or a salt thereof, where R 1  and R 2  are both hydrogen, may be combined with a compound of formula (C 1 -C 6  alkyl)-L′, where L′ is a leaving group such as a chlorine, bromine or iodine atom, in the presence of a base such as butyllithium, and a solvent such as anhydrous THF. Typically the reaction mixture is cooled, e.g. to about 0° C. 
     In another convenient procedure, a compound of formula (I) or a salt thereof, where R 1  and R are both hydrogen, may be combined with a compound of formula L″-COO—(C 1 -C 6  alkyl), where L″ is a leaving group such as a chlorine, bromine or iodine atom, in the presence of a base such as ethylbis(propan-2-yl)amine, and a solvent such as anhydrous dichloromethane. Typically the reaction mixture is heated, e.g. to about 30-50° C. 
     Substituents R 4 , R 5 , R 6  and R 7  may also be modified and/or replaced after the formation of a compound of formula (I). 
     For example, where R 4 , R 5 , R 6  or R 7  represents a halogen atom selected from chlorine, bromine or iodine, the halogen atom may be substituted to arrive at an alternate compound of formula (I). 
     Where the new substituent requires carbon-carbon bond formation, in a convenient procedure a compound of formula (I) where, for example, R 7  represents a chlorine, bromine or iodine atom, may be combined with a boric acid derivative such as R 7a —B(OH) 2 , R 7a —B(pinacole ester) or R 7a —BF 3 —K +  where R 7a  represents the replacement R 7  bonded to the boron atom via a carbon-boron bond, in the presence of a base such as potassium carbonate, caesium carbonate or potassium phosphate, and a metal catalyst such as Pd(o), typically where the metal catalyst is in the form of a transition metal complex such as tetrakis(triphenylphosphine) palladium or di-tert-butyl[dichloro({di-tert-butyl[4-(dimethylamino)phenyl]-phosphaniumyl})palladio][4-(dimethylamino)phenyl] phosphanium. A solvent such as a dioxane/water mixture may be used and the reaction mixture is typically heated, e.g. to around 100-160° C. under conventional heating or microwave irradiation. 
     Where the new substituent requires carbon-nitrogen bond formation, in a convenient procedure a compound of formula (I) where, for example, R 7  represents a chlorine, bromine or iodine atom, may be combined with a primary or secondary amine of formula R 7a H, where R 7a  represents the replacement R 7  and includes a nitrogen atom through which the R 7a  group is to be bonded to the remainder of the compound of formula (I). Examples of R 7a H include morpholine, piperidine, pyrrolidine and substituted derivatives thereof. Optionally, the reaction is performed in the presence of an additional base such as triethylamine or ethylbis(propan-2-yl)amine. A solvent such as ethanol, anhydrous tetrahydrofuran, anhydrous N-methylpyrrolidone or anhydrous N,N-dimethylformamide may be used and the reaction mixture is typically heated, e.g. to around 60-200° C. under conventional heating or microwave irradiation. 
     In a similar procedure, where it is desired to form a carbon-nitrogen bond to a suitable ring nitrogen of a heterocyclic amine, a compound of formula (I) where, for example, R 7  represents a chlorine, bromine or iodine atom, may be combined with the heterocyclic amine in the presence of a base such as sodium hydride and a solvent such as anhydrous N,N-dimethylformamide. The reaction mixture is typically heated, e.g. to around 200° C. under conventional heating or microwave irradiation. 
     Where the new substituent requires carbon-oxygen bond formation, in a convenient procedure a compound of formula (I) where, for example, R 7  represents a chlorine, bromine or iodine atom, may be combined with the desired alcohol in the presence of a base such as sodium hydride and a solvent such as anhydrous tetrahydrofuran. The reaction mixture is typically heated, e.g. to around 60-120° C. under conventional heating or microwave irradiation. 
     The above procedures to substitute R 4 , R 5 , R 6  or R 7 , where R 4 , R 5 , R 6  or R 7  initially represents a leaving group such as a chlorine, bromine or iodine atom, may also be applied to synthesise suitably substituted compounds of formula (IV) or (VI) prior to their reaction with compounds of formula (V). Likewise, they may be applied to the intermediates of formula (II) to replace substituents prior to reaction with an amine of formula (III) or a salt thereof. 
     The compounds of formula (V) where Q is —SO 2 — may conveniently be synthesised by reacting a compound of formula R 3 SO 2 Cl with a compound of formula ClCH 2 CN, in the presence of a reducing agent such as disodium sulfite, and a base such as sodium hydrogen carbonate, in a solvent such as a water/propan-2-ol or water/tetrahydrofuran mixture. The reaction mixture is typically heated, e.g. to around 100-120° C. under conventional heating or microwave irradiation. 
     In an alternate procedure, the compounds of formula (V) where Q is —SO 2 — and R 3  is an amino group attached to the remainder of the compound via the nitrogen atom of the amino group, may be synthesised by reacting the corresponding amine R 3 H with cyanomethanesulfonyl chloride in the presence of a base such as triethylamine and a solvent such as anhydrous dichloromethane. Typically, the reaction is performed at a temperature of from 20-30° C. 
     Compounds of formulae (II), (IV), (V) and (VI) are either commercially available, are known in the literature or may be prepared using known techniques. 
     As already indicated, in the above processes certain functional groups such as phenol, hydroxyl or amino groups in the reagents may need to be protected by protecting groups. Thus, the preparation of the compounds of formula (I) may involve, at an appropriate stage, the introduction and f/or removal of one or more protecting groups. 
     The protection and deprotection of functional groups is described in ‘Protective Groups in Organic Chemistry’, edited by J. W. F. McOmie, Plenum Press (1973) and ‘Protective Groups in Organic Synthesis’, 3rd edition, T.W. Greene and P.G.M. Wuts, Wiley-Interscience (1999). 
     The compounds of formula (I) above may be converted to a pharmaceutically acceptable salt thereof, preferably an acid addition salt such as a hydrochloride, hydrobromide, benzenesulphonate (besylate), saccharin (e.g. monosaccharin), trifluoroacetate, sulphate, nitrate, phosphate, acetate, fumarate, maleate, tartrate, lactate, citrate, pyruvate, succinate, valerate, propanoate, butanoate, malonate, oxalate, 1-hydroxy-2-napthoate (xinafoate), methanesulphonate or p-toluenesulphonate salt. 
     In one aspect of the invention, compounds of formula (I) may bear one or more radiolabels. Such radiolabels may be introduced by using radiolabel-containing reagents in the synthesis of the compounds, or may be introduced by coupling the compounds to chelating moieties capable of binding to a radioactive metal atom. Such radiolabeled versions of the compounds may be used, for example, in diagnostic imaging studies. 
     Unless stated otherwise, any atom specified herein may also be an isotope of said atom. For example, the term “hydrogen” encompasses  1 H,  2 H and  3 H. Similarly carbon atoms are to be understood to include  12 C,  13 C and  14 C, nitrogen atoms are to be understood to include  14 N and  15 N, and oxygen atoms are to be understood to include  16 O,  17 O and  18 O. 
     In a further aspect of the invention, compounds of formula (I) may be isotopically labelled. As used herein, an “isotopically labelled” compound is one in which the abundance of a particular nuclide at a particular atomic position within the molecule is increased above the level at which it occurs in nature. 
     In a still further aspect, the invention provides prodrugs of the compounds of formula (I). The term “prodrug” as used herein refers to a derivative of an active form of a compound which derivative, when administered to a subject, is gradually converted to the active form to produce a better therapeutic response and/or a reduced toxicity level. In general, prodrugs will be functional derivatives of the compounds disclosed herein which are readily convertible in vivo into the compound from which it is notionally derived. Prodrugs include, without limitation, acyl esters, carbonates, phosphates, and urethanes. These groups are exemplary and not exhaustive, and one skilled in the art could prepare other known varieties of prodrugs. Prodrugs may be, for example, formed with available hydroxy, thiol, amino or carboxyl groups. For example, available NH 2  groups in the compounds of the invention may be acylated using an activated acid in the presence of a base, and optionally, in inert solvent (e.g. an acid chloride in pyridine). Conventional procedures for the selection and preparation of suitable prodrugs are described, for example, in “Design of Prodrugs” ed. H. Bundgaard, Elsevier, 1985. 
     Compounds of formula (I) and their salts may be in the form of hydrates or solvates which form an aspect of the present invention. Such solvates may be formed with common organic solvents, including but not limited to, alcoholic solvents e.g. methanol, ethanol or isopropanol. 
     Where compounds of formula (I) are capable of existing in stereoisomeric forms, it will be understood that the invention encompasses the use of all geometric and optical isomers (including atropisomers) of the compounds and mixtures thereof including racemates. The use of tautomers and mixtures thereof also forms an aspect of the present invention. Enantiomerically pure forms are particularly desired. 
     Compounds of formula (I) and their salts may be amorphous or in a polymorphic form or a mixture of any of these, each of which forms an aspect of the present invention. 
     The compounds of formula (I) and their pharmaceutically acceptable salts have activity as pharmaceuticals, in particular as GPR43 receptor agonists and/or as positive allosteric modulators of the GPR43 receptor. Accordingly, they may be used in the treatment of obesity; diabetes, in particular diabetes mellitus such as diabetes mellitus type 1, diabetes mellitus type 2 and gestational diabetes; metabolic syndrome; atherosclerosis; irritable bowel syndrome; and autoimmune diseases including inflammatory bowel disease (e.g. Crohn&#39;s disease and ulcerative colitis), rheumatoid arthritis and systemic lupus. The compounds may also be used in the treatment of asthma, liver fibrosis, non-alcoholic steatohepatitis (NASH), neuroinflammation, multiple sclerosis and colorectal cancer. 
     As used herein, the term “obesity” refers to a person who has a body mass index (BMI) of greater than or equal to 30 kg/m 2 . The BMI may be calculated by dividing a patient&#39;s weight in kilograms by the square of their height in metres (kg/m 2 ). 
     Thus, the present invention provides a compound of formula (I) or a pharmaceutically acceptable salt thereof, as hereinbefore defined, for use in therapy, in particular for the treatment of conditions whose development or symptoms are linked to GPR43 receptor activity. 
     The present invention also provides the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof, as hereinbefore defined, for the preparation of a medicament for the treatment of conditions whose development or symptoms are linked to GPR43 receptor activity. 
     In the context of the present specification, the terms “therapy” and “treatment” also include “prophylaxis” unless there are specific indications to the contrary. The terms “therapeutic”, “therapeutically” and “treating” should be construed accordingly. 
     Prophylaxis is expected to be particularly relevant to the treatment of persons who have suffered a previous episode of, or are otherwise considered to be at increased risk of, the disorder or condition in question. Persons at risk of developing a particular disorder or condition generally include those having a family history of the disorder or condition, or those who have been identified by genetic testing or screening to be particularly susceptible to developing the disorder or condition or those in the prodromal phase of a disorder. 
     In particular, the compounds of the invention (including pharmaceutically acceptable salts) may be used in the treatment of obesity and/or diabetes (including diabetes mellitus such as diabetes mellitus type 1, diabetes mellitus type 2 and gestational diabetes). 
     In one embodiment, the compounds of the invention (including pharmaceutically acceptable salts) may be used in the treatment of obese diabetics, including those suffering from diabetes mellitus type 1, diabetes mellitus type 2 or gestational diabetes. 
     In another embodiment, the compounds of the invention (including pharmaceutically acceptable salts) may be used in the treatment of inflammatory bowel disease. 
     The present invention also provides a method of treating obesity, diabetes (including diabetes mellitus such as diabetes mellitus type 1, diabetes mellitus type 2 and gestational diabetes) or inflammatory bowel disease, which comprises administering to a patient in need thereof a therapeutically effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof as hereinbefore defined. 
     For the above-mentioned therapeutic uses the dosage administered will, of course, vary with the compound employed, the mode of administration, the treatment desired and the disorder indicated. For example, the daily dosage of the compound of the invention, if inhaled, may be in the range from 0.05 micrograms per kilogram body weight (μg/kg) to 100 micrograms per kilogram body weight (μg/kg). Alternatively, if the compound is administered orally, then the daily dosage of the compound of the invention may be in the range from 0.01 micrograms per kilogram body weight (μg/kg) to 100 milligrams per kilogram body weight (mg/kg), preferably from 0.01 to 1 mg/kg body weight 
     The compounds of formula (I) and pharmaceutically acceptable salts thereof may be used on their own but will generally be administered in the form of a pharmaceutical composition in which the formula (I) compound/salt (active ingredient) is in association with a pharmaceutically acceptable adjuvant, diluent or carrier. 
     Therefore the present invention further provides a pharmaceutical composition comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof, as hereinbefore defined, in association with a pharmaceutically acceptable adjuvant, diluent or carrier. 
     The invention still further provides a process for the preparation of a pharmaceutical composition of the invention which comprises mixing a compound of formula (I) or a pharmaceutically acceptable salt thereof as hereinbefore defined with a pharmaceutically acceptable adjuvant, diluent or carrier. 
     Conventional procedures for the selection and preparation of suitable pharmaceutical formulations are described in, for example, “Pharmaceutics—The Science of Dosage Form Design”, M. E. Aulton, Churchill Livingstone, 1988. 
     Pharmaceutically acceptable adjuvants, diluents or carriers that may be used in the pharmaceutical compositions of the invention are those conventionally employed in the field of pharmaceutical formulation, and include, but are not limited to, sugars, sugar alcohols, starches, ion exchangers, alumina, aluminium stearate, lecithin, serum proteins such as human serum albumin, buffer substances such as phosphates, glycerine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes such as protamine sulphate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, polyethylene glycol and wool fat. 
     The pharmaceutical compositions of the present invention may be administered orally, parenterally, by inhalation spray, rectally, nasally, buccally, vaginally or via an implanted reservoir. Oral administration is preferred. The pharmaceutical compositions of the invention may contain any conventional non-toxic pharmaceutically acceptable adjuvants, diluents or carriers. The term parenteral as used herein includes subcutaneous, intracutaneous, intravenous, intramuscular, intra-articular, intrasynovial, intrasternal, intrathecal, intralesional and intracranial injection or infusion techniques. 
     The pharmaceutical compositions may be in the form of a sterile injectable preparation, for example, as a sterile injectable aqueous or oleaginous suspension. The suspension may be formulated according to techniques known in the art using suitable dispersing or wetting agents (such as, for example, Tween 80) and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable diluents and solvents that may be employed are mannitol, water, Ringer&#39;s solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil may be employed including synthetic mono- or diglycerides. Fatty acids, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions. These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant. 
     The pharmaceutical compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, powders, granules, and aqueous suspensions and solutions. These dosage forms are prepared according to techniques well-known in the art of pharmaceutical formulation. In the case of tablets for oral use, carriers which are commonly used include lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried corn starch. When aqueous suspensions are administered orally, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening and/or flavouring and/or colouring agents may be added. 
     The pharmaceutical compositions of the invention may also be administered in the form of suppositories for rectal administration. These compositions can be prepared by mixing the active ingredient with a suitable non-irritating excipient which is solid at room temperature but liquid at the rectal temperature and therefore will melt in the rectum to release the active ingredient. Such materials include, but are not limited to, cocoa butter, beeswax and polyethylene glycols. 
     The pharmaceutical compositions of this invention may be administered by nasal aerosol or inhalation. Such compositions are prepared according to techniques well-known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other solubilising or dispersing agents known in the art. 
     Depending on the mode of administration, the pharmaceutical composition will preferably comprise from 0.05 to 99% w (percent by weight), more preferably from 0.05 to 80% w, still more preferably from 0.10 to 70% w, and even more preferably from 0.10 to 50% w, of active ingredient, all percentages by weight being based on total composition. 
     The compounds of the invention (that is, compounds of formula (I) and pharmaceutically acceptable salts thereof) may also be administered in conjunction with other compounds used for the treatment of the above conditions, for example, biguanide drugs (for example Metformin), insulin (synthetic insulin analogues), oral antihyperglycemics (these are divided into prandial glucose regulators and alpha-glucosidase inhibitors) and sulfonylureas (for example, glimepiride, glibenclamide (glyburide), gliclazide, glipizide, gliquidone, chloropropamide, tolbutamide, acetohexamide, glycopyramide, carbutamide, glibonuride, glisoxepid, glybuthiazole, glibuzole, glyhexamide, glymidine, glypinamide, phenbutamide, tolcylamide and tolazamide). Preferably the sulfonylurea is glimepiride or glibenclamide (glyburide). 
     Alternatively, the compounds of the invention may be administered in combination with a dipeptidyl peptidase-4 (DPP IV) inhibitor (for example, alogliptin); or a phosphodiesterase-4 (PDE4) inhibitor (for example, rolipram, roflumilast or apremilast); or bupropion/naltrexone (“Contrave”); or lorcaserin hydrochloride (“Lorqess”); or phentermine/topiramate (“Qsymia”). 
     The present invention will now be further explained by reference to the following illustrative examples. In the illustrative examples, the compounds synthesised are both named and illustrated structurally. Whilst every effort has been made to ensure that the chemical names and the chemical structures are consistent, if any inconsistencies occur the illustrated chemical structure should be taken to be correct, unless the illustrated chemical structure is chemically impossible. 
     The methods used for the synthesis of the compounds of the invention are illustrated by the general schemes below and the preparative examples that follow. The starting materials and reagents used in preparing these compounds are available from commercial suppliers. These general schemes are merely illustrative of methods by which the compounds of this invention can be synthesised, and various modifications to these schemes can be made and will be suggested to one skilled in the art having referred to this disclosure. 
    
    
     EXPERIMENTAL 
     Nuclear magnetic resonance (NMR) spectra were recorded at 400 MHz or 300 MHz as stated and at 300.3K unless otherwise stated; the chemical shifts (δ) are reported in parts per million. Spectra were recorded using a Bruker 400 AVANCE instrument fitted with a 5 mm BBFO probe with instrument controlled by Bruker TopSpin 2.1 software, or by a Bruker 400 AVANCE-III instrument fitted with a 5 mm BBFO probe with instrument controlled by Bruker TopSpin 3.0 software, or by a Bruker 300 MHz AVANCE II instrument fitted with a 5 mm DUL probe with instrument controlled by Bruker TopSpin 1.3 software. 
     Purity was assessed using one or more of the following:
         UPLC with UV (photodiode array) detection over a wide range of wavelengths, normally 220-450 nm, using a Waters Acquity UPLC system equipped with Acquity UPLC BEH or HSS C18 columns (2.1 mm id×50 mm long) operated at 50 or 60° C. Mobile phases typically consisted of acetonitrile or methanol mixed with water containing either 0.1% formic acid or 0.025% ammonia. Mass spectra were recorded with a Waters SQD single quadrupole mass spectrometer using atmospheric pressure ionisation.   UPLC with UV (photodiode array) detection over a wide range of wavelengths, normally 200-500 nm, using a Waters Acquity H-Class UPLC system controlled by Empower-2 software. Mass spectra were recorded with a Waters SQD single quadrupole mass spectrometer using electro spray ionization. Mobile phase consisted of 5 mm Ammonium Acetate containing 0.1% formic acid in Water and Acetonitrile using Acquity UPLC BEH or HSS C18 columns (2.1 mm id×50 mm long).   LCMS with UV (photodiode array) detection over a wide range of wavelengths, normally 200-500 nm and the detection was also proceed at wavelength 260 nm and 80 bandwidth, using Shimandzu Nexera LCMS-2020 system controlled by Lab Solution software. Mass spectra were recorded with a single quadrupole mass spectrometer using electro spray ionization. Mobile phase consisted of 20 mm Ammonium Acetate mixed with water and Methanol using Waters X-bridge column (C18, 5 μm, 4.6 mm id×150 mm).   LCMS with UV (photodiode array) detection over a wide range of wavelengths, normally 200-500 nm, using Waters ZQ-2000 system controlled by Empower-1 software. Mass spectra were recorded with a Waters ZQ single quadrupole mass spectrometer using electro spray ionization. Mobile phases consisted of 0.1% Ammonia mixed with water and Acetonitrile using Waters X-bridge column (C18, 5 μm, 4.6 mm id×150 mm).       

     Compounds were purified using normal phase chromatography on silica, using Biotage or Isolute KP-Sil cartridges or Kinesis Telos Silica cartridges, or on basic silica, using Biotage or Isolute KP-NH cartridges, or by reverse phase chromatographic methods, using Biotage or Isolute KP-C18-HS cartridges or by SCX-2 catch-release cartridges, or by Preparative HPLC. 
     Preparative HPLC was performed using one or more of the following:
         Agilent Technologies 1100 Series system or a Waters autopurification LC/MS system typically using Waters 19 mm id×250 mm long C18 columns such as XBridge or SunFire 5 μm materials at room temperature.   Shimadzu Preparative HPLC system typically using 19 mm id×150 mm long C18 columns 5 μm or 20 mm id×250 mm long C8 columns 5 μm materials at room temperature. Shimadzu Preparative HPLC system controlled by LC-Solution software.       

     Mobile phases typically consisted of acetonitrile or methanol mixed with water containing either 0.1% formic acid or 0.1% ammonia, unless stated otherwise. 
     Room temperature in the following examples means the temperature ranging from 20° C. to 25° C. 
     The abbreviations used in the specific examples have the following meanings:
     Ac acetyl   aq aqueous   Bn, Bzl benzyl   BOC, Boc tert-butoxycarbonyl   bp boiling point,   br broad (spectral)   Bu, n-Bu normal (primary) butyl   t-Bu tert-butyl   Bz benzoyl   CBZ, Cbz benzyloxycarbonyl   CD 2 Cl 2  deuterated dichloromethane   CDCl 3  deuterated chloroform   CHCl 3  chloroform   m-CPBA meta-chloroperoxybenzoic acid   Cy cyclohexyl   δ chemical shift in ppm downfield from tetramethylsilane   d day(s); doublet (spectral);   DCE 1,2-dichloroethane   DCM dichloromethane   DMAP 4-(N,N-dimethylamino)pyridine   DME 1,2-dimethoxyethane   DMF dimethylformamide   DMSO dimethyl sulfoxide   DMSO-d 6  perdeuterated dimethyl sulfoxide   DPPF 1,1′-bis(diphenylphosphanyl) ferrocene   ES electrospray   Et ethyl   H-frit Biotage Phase Separator (Part #120-1908-F)   h hour(s)   HPLC high-performance liquid chromatography   Hz hertz   L litre(s)   LDA lithium diisopropylamide   μ micro   m multiplet (spectral); metre(s); milli   M molar (moles per litre); mega   Me methyl   mg milligram   MgSO 4  magnesium sulfate   min minute(s); minimum   mL millilitre   mmol millimoles   mmolar millimolar (millimoles per liter)   mol mole(s); molecular (e.g. in mol. wt.)   mp melting point   Ms, mesyl methylsulfonyl   MS mass spectrometry   MTBE methyl tert-butyl ether   MW molecular weight   m/z mass-to-charge ratio   NaHCO 3  sodium bicarbonate; sodium hydrogen carbonate   NaHMDS sodium hexamethyldisilazane   nm nanometer(s)   NMP N-methylpyrrolidone   NMR nuclear magnetic resonance   Pd(amphos) 2 Cl 2  Bis(di-tert-butyl(4-dimethylaminophenyl)phosphine) dichloropalladium(II)   Ph phenyl   PMB p-methoxybenzyl   ppm part(s) per million   Pr, n-Pr propan-1-yl   iPr isopropyl   q quartet (spectral)   rt room temperature   s singlet (spectral); second(s)   Sat. saturated   t triplet (spectral)   t time; temperature in units of degrees Celsius (° C.)   TEA triethylamine   Tf, trifyl trifluoromethanesulfonyl   TFA trifluoroacetic acid   TFAA trifluoroacetic anhydride   THF tetrahydrofuran   THP tetrahydropyran-2-yl   TMEDA N,N,N′,N′-tetramethyl-1,2-ethylenediamine   Ts, tosyl para-toluenesulfonyl   UV ultraviolet   

     1. Intermediates 
     
       
         
         
             
             
         
       
     
     Intermediate 1 2-(benzenesulfonyl)-2-(3-chloropyrzin-2-yl)acetonitrile 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2,3-dichloropyrazine (CAS 4858-85-9; 1.4 mL, 13 mmol) and 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 24 g, 13 mmol) in DMSO (8 mL) was added DBU (4.1 mL, 27 mmol). The reaction was subjected to microwave irradiation at 100° C. for 45 mins. The reaction mixture was diluted with water and brine and then extracted with ethyl acetate. The aqueous phase was extracted further with DCM. The combined organics were dried over MgSO 4  and concentrated in vacuo. The crude product was loaded onto a plug of silica (10 g) and eluted using 0-100% EtOAc/petroleum ether. The product fractions were concentrated in vacuo to afford the title compound. 
       1 H NMR (400 MHz, DCM-d 2 ) δ ppm 6.01 (s, 1H) 7.62-7.72 (m, 2H) 7.83-7.91 (m, 3H) 8.51-8.59 (m, 2H) 
     MS ES + : 294 
     
       
         
         
             
             
         
       
     
     Intermediate a 2-(3-chloropyrazin-2-yl)-2-(4-methylbenzenesulfonyl)acetonitrile 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2,3-dichloropyrazine (CAS 4858-85-9; 360 μL, 3-4 mmol) and 2-(4-methylbenzenesulfonyl)acetonitrile (CAS 5697-44-9; 736 mg, 3.8 mmol) in acetonitrile (7 mL) was added DBU (620 μL, 4.1 mmol). The reaction was heated in a microwave at 80° C. for 30 min. The reaction mixture was evaporated to dryness and purified by column chromatography (C18-silica 0-30% Acetonitrile+0.05% NH 3 /Water+0.1% NH 3 ) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 2.46 (s, 3H) 7.00 (s, 1H) 7.50 (d, J=1 Hz, 2H) 7.62 (d, J=1 Hz, 2H) 8.64-8.75 (m, 2H) 
     MS ES + : 308 
     
       
         
         
             
             
         
       
     
     Intermediate 3 2-(4-chlorobenzenesulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2,3-dichloropyrazine (CAS 4858-85-9; 156 μL, 1.50 mmol) and 2-(4-chlorophenylsulfonyl)acetonitrile (CAS 1851-09-8; 323 mg, 1.50 mmol) in DMF (1 mL) was added DBU (452 μL, 3.00 mmol). The reaction was heated in a microwave at 100° C. for 30 min. The reaction mixture was diluted with ammonium chloride solution, extracted with EtOAc/tetrahydrofuran (2:1), the combined organics dried (H frit) and evaporated to dryness. The crude product was purified by column chromatography silica (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 328 
     
       
         
         
             
             
         
       
     
     Intermediate 4 2-(3-chloropyrazin-1-yl)-2-(4-fluorobenzenesulfonyl)acetonitrile 
     
       
         
         
             
             
         
       
     
     Prepared as described for 2-(4-chlorobenzenesulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile (Intermediate 3), to a stirred solution of 2,3-dichloropyrazine (CAS 4858-85-9; 156 μL, 1.50 mmol) and 2-(4-fluorophenylsulfonyl)acetonitrile (CAS 32083-66-2; 299 mg, 1.50 mmol) in DMF (1 mL) was added DBU (452 μL, 3.00 mmol). 
     The reaction was heated in a microwave at 100° C. for 30 min. The crude product was purified by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 312 
     
       
         
         
             
             
         
       
     
     Intermediate 5 2-(3-chloropyrazin-2-yl)-2-[4-(propan-2-yloxy)benzenesulfonyl]acetonitrile 
     
       
         
         
             
             
         
       
     
     Prepared as described for 2-(4-Chlorobenzenesulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile (Intermediate 3), to a stirred solution of 2,3-dichloropyrazine (CAS 4858-85-9; 156 μL, 1.50 mmol) and 2-(4-isopropoxyphenylsulfonyl)acetonitrile (CAS 886499-39-4; 359 mg, 1.50 mmol) in DMF (1 mL) was added DBU (452 μL, 3.00 mmol). The reaction was then heated in a microwave at 100° C. for 30 min. The crude product was purified by column chromatography (silica, 0-40% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 352 
     
       
         
         
             
             
         
       
     
     Intermediate 6 2-(3-chloropyrazin-2-yl)-2-(thiophene-2-sulfonyl)acetonitrile 
     
       
         
         
             
             
         
       
     
     Prepared as described for 2-(4-chlorobenzenesulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile (Intermediate 3), to a stirred solution of 2,3-dichloropyrazine (CAS 4858-85-9; 156 μL 1.50 mmol) and 2-(thiophen-2-ylsulfonyl)acetonitrle (CAS 175137-62-9; 281 mg, 1.50 mmol) in DMF (1 mL) was added DBU (452 μL, 3.00 mmol). The reaction was heated in a microwave at 100° C. for 30 min then 125° C. for 30 min. The crude product was purified by column chromatography (C18-silica, 0-30% Acetonitrile+0.05% NH 3 /Water+0.1% NH 3 ) to afford the title compound. 
     MS ES + : 300. 
     
       
         
         
             
             
         
       
     
     Intermediate 7 2-bromo-N-cyclohexylpyridin-3-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of cyclohexanone (CAS 108-94-1; 851 mg, 8.67 mmol) and 2-bromopyridin-3-amine (CAS 39856-58-1; 500 mg, 2.89 mmol) in DCM (8 mL) at 0° C. under N 2  was added TiCl 4  solution (1M in DCM, 3.18 mL, 3.18 mmol) dropwise. The reaction mixture was allowed to stir for 2 hours at rt and then cooled to 0° C. Sodium triacetoxyborohydride (1.8 g, 8.67 mmol) was added portionwise and then the reaction allowed to warm to rt and stirred over a weekend. The reaction mixture was quenched slowly into water and then extracted with DCM. The organic phase was separated and concentrated in vacuo. The crude product was purified by column chromatography (silica, 0-40% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 255 
     
       
         
         
             
             
         
       
     
     Intermediate 8 2-(4-methylbenzenesulfonyl)-2-(3-nitropyridin-2-yl)acetonitrile 
     
       
         
         
             
             
         
       
     
     To a stirred solution of potassium tert-butoxide (3.5 g, 32 mmol) in propan-2-ol (25 mL) at 0° C. was added 2-(4-methylbenzenesulfonyl)acetonitrile (CAS 5697-44-9; 3.69 g, 18 mmol) and the resulting mixture stirred for 30 min. 2-chloro-3-nitropyridine (CAS 5470-18-8; 2.5 g 15.8 mmol) was added and the reaction mixture stirred at 65° C. for 6 h. The reaction mixture was allowed to cool and concentrated in vacuo. The resulting residue was taken up in water and extracted with ethyl acetate. The organic phase was dried (Na 2 SO 4 ) and concentrated in vacuo. The crude product was purified by column chromatography (silica, 25-30% EtOAc/hexane) to afford the title compound. 
       1 H NMR (4 MHz, DMSO-d 6 ) δ ppm 2.45 (s, 3H), 6.93 (s, 1H), 7.45-7.55 (m, 2H), 7.55-7.65 (m, 3H), 8.05-8.15 (m, 1H), 8.50-8.60 (m, 1H) 
     MS ES + : 318 
     Intermediate 9 2-amino-3-(4-methylbenzenesulfonyl)-1H-pyrrolo[3,2-b]pyridin-1-ol 
     
       
         
         
             
             
         
       
     
     A stirred suspension of 2-(4-methylbenzenesulfonyl)-2-(3-nitropyridin-2-yl)acetonitrile (Intermediate 8; 1.2 g, 11 mmol) and palladium on carbon (10% w/w) (60 mg, 0.55 mmol) in acetic acid (0.5 mL) and ethyl acetate (50 mL) was placed under an atmosphere of hydrogen. The reaction was stirred at rt for to h. The reaction was filtered and the filtrate concentrated in vacuo. The residue was taken up in water and neutralised with sat. aq. NaHCO 3  solution and then extracted with ethyl acetate. The organics were dried (NaSO 4 ) and concentrated in vacuo. The crude product was purified by column chromatography (silica, 2-5% MeOH/DCM) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 2.30 (s, 3H), 6.90-7.00 (m, 1H), 7.10 (s, 2H), 7.25-7.35 (m, 2H), 7.35-7.45 (m, 1H), 7.85-7.95 (m, 2H), 8.05-8.15 (m, 1H), 11.50 (s, 1H) 
     MS ES + : 304 
     Intermediate 10 3-(4-methylbenzenesulfonyl)-1H-pyrrolo[3,2-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     A stirred suspension of 2-amino-3-(4-methylbenzenesulfonyl)-1H-pyrrolo[3,2-b]pyridin-1-ol (Intermediate 9; 400 mg) and palladium on carbon (10% w/w) (50 mg) in acetic acid (2 mL) and ethyl acetate (10 mL) was placed under an atmosphere of hydrogen at too psi. After 8 h the reaction was diluted with ethyl acetate and filtered. The filtrate was washed with sat. aq. NaHCO 3  solution and the organic phase separated and dried (Na 2 SO 4 ). The organic phase was concentrated in vacuo. The crude product was triturated with hexane and filtered to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 2.32 (s, 3H) 6.82-6.97 (m, 3H) 7.27-7.40 (m, 3H) 7.86-7.96 (m, 2H) 8.02-8.09 (m, 1H) MS ES + : 288 
     
       
         
         
             
             
         
       
     
     Intermediate 11 2-(2-chloropyridin-3-yl)-2-(4-methylbenzenesulfonyl)acetonitrile 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2-chloro-3-iodopyridine (CAS 78607-36-0; 4.9 g, 20.5 mmol) in toluene(15 mL) was added potassium tert-butoxide (2.81 g, 25.0 mmol), Pd 2 dba 3  (1.53 g, 1.70 mmol) and 2-(4-methylbenzenesulfonyl)acetonitrile (CAS 5697-44-9; 2.64 g, 14.6 mmol). The reaction was heated at 125° C. for 4 h. The reaction was poured onto ice and extracted with ethyl acetate. The organic phase was separated, dried and concentrated in vacuo. The crude product was purified by column chromatography (silica, 20-22% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 2.45 (s, 3H), 6.76 (s, 1H), 7.50-7.58 (m, 2H), 7.60-7.65 (m, 1H), 7.65-7.75 (m, 2H), 7.90-8.00 (m, 1H), 7.55-7.65 (m, 1H) 
     MS ES + : 307 
     
       
         
         
             
             
         
       
     
     Intermediate 12 2-(3-chloropyrazin-2-yl)-2-(4-methoxybenzenesulfonyl)acetonitrle 
     
       
         
         
             
             
         
       
     
     A neat mixture of 2,3-dichloropyrazine (CAS 4858-85-9; 0.100 mL, 0.958 mmol), 2-((4-methoxyphenyl)sulfonyl)acetonitrile (CAS 132276-87-0; 220 mg, 0.958 mmol) and DBU (0.289 mL, 1.916 mmol) was heated to 85° C. for 1.5 h. The reaction mixture was treated with dilute citric acid and EtOAc. The phases were separated and the aqueous extracted with EtOAc. The combined organic extracts were then washed with dilute citric acid, water, sat. NaHCO 3 , sat. brine, dried (H-frit) and evaporated. The crude material was absorbed onto MgSO 4  from DCM/MeOH and purified by column chromatography (silica, 0-40% EtOAc/petroleum ether) to afford the title compound.  1 H NMR (400 MHz, DCM-d 2 ) δ ppm 3.96 (s, 3H) 5.99 (s, 1H) 7.10 (d, J=9 Hz, 2H) 7.76 (d, J=9 Hz, 2H) 8.49-8.60 (m, 2H) 
     MS ES + : 324 
     
       
         
         
             
             
         
       
     
     Intermediate 13 2-(2-chloropyridin-3-yl)-2-(4-methoxybenzenesulfonyl)acetontrile 
     
       
         
         
             
             
         
       
     
     To a stirred degassed solution of Pd(Ph 3 P) 4  (0.058 g, 0.050 mmol) in anhydrous DME (1.5 mL) under an atmosphere of nitrogen was added a solution of 2-((4-methoxyphenyl)sulfonyl)acetonitrile (CAS 132276-87-0; 0.232 g, 1.10 mmol) and NaH (0.084 g, 2.10 mmol) in anhydrous DME (4 mL). The resulting mixture was stirred at room temperature for to min followed by the addition of 2-chloro-3-iodopyridine (CAS 78607-36-0; 00.239 g, 1 mmol). The reaction was heated in a microwave at 90° C. to 110° C. for 2.5 h. More Pd(Ph 3 P) 4  (0.029 g, 0.025 mmol) was added and the reaction heated in a microwave at 115° C. to 120° C. for 1.5 h. The solvent was removed under reduced pressure and the residue was diluted with water, neutralised with 2 M aq. HCl solution and extracted with DCM. The combined organic phases were washed with brine, dried over MgSO 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (silica, 10-40% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 3.90 (s, 3H) 5.72 (s, 1H) 7.03 (d, J=9 Hz, 2H) 7.33-7.45 (m, 1H) 7.74 (d, J=9 Hz, 2H) 7.89-7.99 (m, 1H) 8.41-8.55 (m, 1H) MS ES + : 323 
     
       
         
         
             
             
         
       
     
     Intermediate 2-(benzenesulfonyl)-2-(2-chloropyridin-3-yl)acetonitrile 
     
       
         
         
             
             
         
       
     
     To a stirred degassed solution of Pd(Ph 3 P) 4  (0.116 g, 0.100 mmol) in anhydrous DME (1.5 mL) under an atmosphere of nitrogen was added a solution of 2-(phenylsulfonyl)acetonitrile (0.399 g, 2.20 mmol) and NaH (0.168 g, 4.20 mmol) in anhydrous DME (4 mL). The resulting mixture was stirred at room temperature for to min followed by the addition of 2-chloro-3-iodopyridine (0.479 g, 2.00 mmol). The reaction mixture was heated at 120° C. for 1.5 h. The solvent was removed under reduced pressure and the residue was diluted with water, neutralised with 2 M aq. HCl solution and extracted with DCM. The combined organic phases were washed with brine, dried over MgSO 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (silica, 10-40% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (4 MHz, CHLOROFORM-d) δ ppm 5.73 (s, 1H) 7.36-7.45 (m, 1H) 7.56-7.71 (m, 2H) 7.76-7.86 (m, 1H) 7.87-7.94 (m, 2H) 7.95-8.03 (m, 1H) 8.45-8.60 (m, 1H) 
     MS ES + : 293 
     
       
         
         
             
             
         
       
     
     Intermediate 15 N-cyclohexyl-5-iodopyrimidin-4-amine 
     
       
         
         
             
             
         
       
     
     A stirred suspension of cyclohexanamine (CAS 108-91-8; 0.114 mL, 0.998 mmol), 4-chloro-5-iodopyrimidine (CAS 63558-65-6; 200 mg, 0.832 mmol) and Cs 2 CO 3  (407 mg, 1.248 mmol) in N-methyl-2-pyrrolidinone (2 mL) was heated in a microwave at 100° C. for 1 h. The reaction mixture was poured into water and extracted with EtOAc (×2). The combined extracts were washed with water, dilute citric acid, water, sat. NaHCO 3 , sat. brine, dried (H-frit) and evaporated. The crude product was then purified by column chromatography (silica, 0-20% EtOAc/petroleum ether) to afford the title compound.  1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.21-1.37 (m, 3H) 1.39-1.54 (m, 2H) 1.63-1.73 (m, 1H) 1.73-1.85 (m, 2H) 1.99-2.12 (m, 2H) 3.96-4.10 (m, 1H) 5.19 (br. s., 1H) 8.44 (s, 1H) 8.46 (s, 1H) 
     MS ES + : 304 
     
       
         
         
             
             
         
       
     
     Intermediate 16 4-chloro-N-cyclohexylpyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 4-chloropyrimidin-5-amine (CAS 54660-78-5; 150 mg, 1.16 mmol) and cyclohexanone (CAS 108-94-1; 360 μL, 3.47 mmol) in DCM (5 mL) at 0° C. was added TiCl 4  solution (1.0M in DCM, 1.27 mL, 1.27 mmol). The reaction was stirred at room temperature for 2 h. Sodium triacetoxyborohydride (736 mg, 3.47 mmol) was then added portionwise. Stirring at rt was maintained for 2 h. The reaction mixture was poured into water and extracted with EtOAc (×2). The combined organic extracts were washed with water, sat. NaHCO 3 , sat.brine, dried (H-frit) and evaporated. The crude product was purified by column chromatography (silica, 0-15% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.21-152 (m, 5H) 1.62-1.96 (m, 3H) 1.99-2.17 (m, 2H) 3.29-3.47 (m, 1H) 4.11-4.27 (m, 1H) 8.06 (s, 1H) 8.33 (s, 1H) 
     MS ES + : 212 
     
       
         
         
             
             
         
       
     
     Intermediate 17 N-cyclohexyl-4-iodopyridin-3-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of cyclohexanone (CAS 108-94-1; 1.34 g, 13.6 mmol) and 4-iodopyridin-3-amine (CAS 105752-11-2; 1 g, 4.55 mmol) in DCM (15 mL) at 0° C. under N 2  was added TiCl 4  solution (1.0M in DCM, 5.00 mL, 5.00 mmol) dropwise. The reaction mixture was allowed to stir at rt for 2 hours and then sodium triacetoxyborohydride (2.89 g, 13.6 mmol) was added portionwise. The reaction mixture was allowed to stir at room temperature overnight. The reaction mixture was quenched slowly into water and then extracted with DCM. The organics were separated and concentrated. The crude product was purified by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.07-1.49 (m, 4H) 1.56-1.76 (m, 4H) 1.89-1.97 (m, 2H) 3.42-3.53 (m, 1H) 4.28 (d, J=8 Hz, 1H) 7.48 (d, J=5 Hz, 1H) 7.65 (d, J=5 Hz, 1H) 7.90 (s, 1H) 
     MS ES + : 303 
     
       
         
         
             
             
         
       
     
     Intermediate 18 2-bromo-N-(4,4-difluorocyclohexyl)pyridin-3-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to a stirred solution of 4,4-difluorocyclohexanone (CAS 22515-18-0; 2.33 g, 17.3 mmol) and 2-bromopyridin-3-amine (CAS 39856-58-1; 1 g, 5.78 mmol) in DCM (15 mL) at 0° C. under N 2  was added TiCl 4  solution (1M in DCM, 6.36 mL, 6.36 mmol) dropwise. The reaction was allowed to stir at room temperature for 2 h and then cooled to 0° C. Sodium triacetoxyborohydride (3.68 g, 17.3 mmol) was added portionwise and then the reaction stirred at room temperature for 72 h. The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 291 
     
       
         
         
             
             
         
       
     
     Intermediate 19 3-bromo-N-cyclohexylpyrldin-4-amine 
     
       
         
         
             
             
         
       
     
     A neat mixture of 3-bromo-4-fluoropyridine (200 mg 1.14 mmol) and cyclohexanamine (CAS 108-91-8; 650 μL, 5.68 mmol) was heated in a microwave at 120° C. for 45 min. The reaction mixture was dissolved in EtOAc and washed with water, brine, dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude product was purified by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.24-1.49 (m, 4H) 1.60-1.73 (m, 2H) 1.74-1.88 (m, 2H) 1.95-2.16 (m, 2H) 3.18-3.46 (m, 1H) 4.71 (br. s, 1H) 6.48 (d, J=6 Hz, 1H) 8.12 (d, J=6 Hz, 1H) 8.34 (s, 1H) 
     MS ES + : 255 
     
       
         
         
             
             
         
       
     
     Intermediate 20 methyl N-[7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-yl]-N-(methoxycarbonyl)carbamate 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine (Example 1; 0.135 g, 0.38 mmol) in anhydrous THF (5 mL) at −78° C. and under an atmosphere of nitrogen was added dropwise a solution of butyllithium (0.152 mL, 0.380 mmol) in hexanes (2.5 M). The resulting mixture was stirred at −78° C. for 10 min and then quenched at −78° C. by the addition of methyl carbonochloridate (0.294 ml, 3.80 mmol) and allowed to warm to room temperature. The reaction was partitioned between diethyl ether and water. The phases were separated and the aqueous extracted with diethyl ether. The combined organics were dried over MgSO 4 , filtered and concentrated in vacuo. Purification was performed by chromatography (preparative HPLC, 40-80% acetonitrile/water (with 0.1% formic acid)) to afford the title compound. 
       1 H NMR (3 MHz, CHLOROFORM-d) δ ppm 1.18-1.48 (m, 3H) 1.65-1.99 (m, 5H) 2.40-2.67 (m, 2H) 3.76 (s, 6H) 4.04-4.29 (m, 1H) 7.39-7.65 (m, 3H) 8.07-8.25 (m, 2H) 8.38 (d, J=2 Hz, 1H) 8.66 (d, J=2 Hz, 1H) 
     MS ES + : 473 
     
       
         
         
             
             
         
       
     
     Intermediate 21 3-bromo-((4,4-difluorocyclohexyl)amino)-6-methylpyridine 1-oxide 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 3-bromo-2-chloro-6-methylpyridine 1-oxide (CAS 185017-76-9; 0.309 &amp; 1.39 mmol) and difluorocyclohexanamine hydrochloride (CAS 675112-70-6; 0.309 g, 1.80 mmol) in NMP (3 mL) was added Cs 2 CO 3  (1.22 g, 3.74 mmol) and the resulting mixture was heated at 110° C. to 140° C. for 6 h using a microwave reactor. The mixture was partitioned between ethyl acetate and water. The phases were separated and the aqueous extracted with ethyl acetate (×2) The combined organics were washed with water, brine, dried over MgSO 4 , filtered and concentrated under reduced pressure. 
     The crude product was purified by column chromatography (silica, 20-100% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 321 
     Intermediate 22 2-amino-1-(4,4-difluorocyclohexyl)-6-methyl-3-(phenylsulfonyl)-1H-pyrrolo[2,3-b]pyridine 7-oxide 
     
       
         
         
             
             
         
       
     
     To a stirred degassed solution of Pd(Ph 3 P) 4  (18 mg, 0.016 mmol) in anhydrous DME (1 mL) under an atmosphere of nitrogen was added a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 62 mg, 0.343 mmol) and NaH, 60% dispersion in oil (26 mg, 0.654 mmol) in anhydrous DME (1 mL). The resulting mixture was stirred at room temperature for 10 min followed by addition of a solution of 3-bromo-2-((4,4-difluorocyclohexyl)amino)-6-methylpyridine 1-oxide (Intermediate 21; 1 mg, 0.311 mmol) in anhydrous DME (1 mL). The reaction mixture was heated at 120° C. for 1.5 h. The solvent was removed under reduced pressure and the residue was diluted with water, neutralised with 2 M aq. HCl solution and extracted with DCM. The combined organic phases were washed with brine, dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude product was purified by column chromatography (silica, 0-10% MeOH/DCM) to afford the title compound. 
     MS ES + : 422 
     
       
         
         
             
             
         
       
     
     Intermediate 23 4-chloro-N-cyclohexyl-6-methoxypyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to a stirred solution of 4-chloro-6-methoxypyrimidin-5-amine (CAS 15846-19-2; 0.15 g, 0.940 mmol) and cyclohexanone (CAS 108-94-1; 0.294 ml, 2.82 mmol) in anhydrous DCM (5 mL) under an atmosphere of nitrogen at 0° C. was added TiCl 4  solution (1M in DCM, 3.66 mL, 3.66 mmol). The reaction was stirred at room temperature for 2 h. Sodium triacetoxyborohydride (1.94 g, 9.15 mmol) was added portionwise and the reaction stirred at room temperature for 16 h. The crude product was purified by column chromatography (silica, 0-20% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 242 
     
       
         
         
             
             
         
       
     
     Intermediate 24 4-bromo-6-chloro-N-cyclohexylpyridazin-3-amine 
     
       
         
         
             
             
         
       
     
     To stirred solution of cyclohexanone (CAS 108-94-1; 1060 mg, 10.8 mmol) and 4-bromo-6-chloropyridazin-3-amine (CAS 446273-59-2; 750 mg, 3.60 mmol) in THF (10 mL) at 0° C. under N 2  was added titanium isopropoxide (IV) (1.16 mL, 3.96 mmol) dropwise. The reaction was allowed to stir at room temperature for 2 h and then cooled to 0° C. Sodium triacetoxyborohydride (4580 mg, 21.6 mmol) was added portionwise and then the reaction allowed to stir at room temperature. The reaction was poured into water and extracted with DCM. The organics were separated and concentrated. 
     The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. MS ES + : 292 
     
       
         
         
             
             
         
       
     
     Intermediate 25 4-chloro-N-(4,4-difluorocyclohexyl)-6-ethoxypyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to stirred solution of 4,4-difluorocyclohexanone (CAS 22515-18-0; 1480 mg, 11.1 mmol) and 4-chloro-6-ethoxypyrimidin-5-amine (CAS 63291-59-8; 960 mg, 5.53 mmol) in DCM (15 mL) at 0° C. under N 2  was added TiCl 4  solution (1M in DCM, 6.08 mL, 6.08 mmol) dropwise. The reaction was allowed to stir at room temperature for 2 h and then cooled to 0° C. Sodium triacetoxyborohydride (2340 mg, 11.06 mmol) was added portionwise and then the reaction allowed to stir at room temperature overnight. The crude product was purified by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.33-1.41 (m, 3H) 1.51-1.64 (m, 2H) 1.77-1.89 (m, 4H) 2.00-2.09 (m, 2H) 3.66-3.81 (m, 1H) 4.39-4.47 (m, 3H) 8.08 (s, 1H) MS ES + : 292 
     
       
         
         
             
             
         
       
     
     Intermediate 26 4-(benzyloxy)-6-chloropyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of phenylmethanol (CAS 100-51-6; 791 mg, 7.32 mmol) in THF (10 mL) at 0° C. was added NaH, 60% dispersion in oil (0.305 g, 7.62 mmol) portionwise. The resulting suspension was allowed to stir for 15 minutes. 4,6-dichloropyrimidin-5-amine (CAS 5413-85-4; 1 g, 6.10 mmol) was then added slowly and the reaction allowed to warm to room temperature and stirred overnight. The reaction mixture was poured into water and extracted with DCM. The phases were separated and the organics concentrated in vacuo to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 5.45 (s, 2H) 5.49 (s, 2H) 7.31-7.36 (m, 1H) 7.38-7.44 (m, 2H) 7.47-7.52 (m, 2H) 7.92 (s, 1H) MS ES + : 236 
     Intermediate 27 4-(benzyloxy)-6-chloro-N-(4,4-difluorocyclohexyl)pyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to stirred solution of 4,4-difluorocyclohexanone (CAS 22515-18-0; 1.59 g, 11.9 mmol) and 4-(benzyloxy)-6-chloropyrimidin-5-amine (Intermediate 26; 1.4 g, 5.94 mmol) in DCM (15 mL) at 0° C. under N 2  was added TiCl 4  solution (1M in DCM, 6.53 mL, 6.53 mmol) dropwise. The reaction was allowed to stir at room temperature for 2 h and then cooled to 0° C. Sodium triacetoxyborohydride (2.52 g, 11.9 mmol) was added portionwise and then the reaction allowed to stir at room temperature overnight. The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.74-1.82 (m, 2H) 1.89-1.98 (m, 2H) 2.26-2.39 (m, 2H) 2.40-2.46 (m, 2H) 3.64-3.78 (m, 1H) 4.47-4.53 (m, 1H) 5.47 (s, 2H) 7.30-7.46 (m, 3H) 7.46-7.54 (m, 2H) 8.12 (s, 1H) 
     MS ES + : 354 
     
       
         
         
             
             
         
       
     
     Intermediate 28 6-chloro-5-N-cyclohexyl-4-N,4-N-dimethylpyrimidine-4,5-diamine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to a stirred solution of 6-chloro-N 4 ,N 4 -dimethylpyrimidine-4,5-diamine (CAS 130623-81-3; 560 mg, 3.24 mmol) and cyclohexanone (CAS 108-94-1; 1.016 mL, 9.73 mmol) in anhydrous DCM (18 mL) under an atmosphere of N 2  at 0° C. was added dropwise TiCl 4  solution (1M in DCM, 3.66 mL, 3.66 mmol). The reaction was stirred at room temperature for 2 h. Sodium triacetoxyborohydride (1.94 g, 9.15 mmol) was added portionwise and the reaction stirred at room temperature for 16 h. The crude product was purified by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 255 
     
       
         
         
             
             
         
       
     
     Intermediate 29 4-chloro-N-cyclopentyl-6-methoxypyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to a stirred solution of 4-chloro-6-methoxypyrimidin-5-amine (CAS 15846-19-2; 200 mg, 1.25 mmol) and cyclopentanone (CAS 120-92-3; 0.33 mL, 3.76 mmol) in anhydrous DCM (6 mL) under an atmosphere of N 2  at 0° C. was added dropwise TiCl 4  solution (1M in DCM, 1.4 mL, 1.38 mmol). The reaction was stirred at room temperature for 2 h. Sodium triacetoxyborohydride (797 mg, 3.76 mmol) was added portionwise and the reaction stirred at room temperature for 16 h. The crude product was purified by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.33-1.53 (m, 2H) 1.55-1.82 (m, 4H) 1.83-2.00 (m, 2H) 3.73 (d, J=9 Hz, 1H) 4.04 (s, 3H) 4.18-4.42 (m, 1H) 8.08 (s, 1H) MS ES + : 228 
     
       
         
         
             
             
         
       
     
     Intermediate 30 4-chloro-N-cyclohexyl-2-methoxypyridin-3-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to a stirred solution of 4-chloro-2-methoxypyridin-3-amine (CAS 934180-49-1; 250 mg, 1.58 mmol) and cyclohexanone (CAS 108-94-1; 309 mg, 3.15 mmol) in anhydrous DCM (10 mL) under an atmosphere of N 2  at 0° C. was added dropwise TiCl 4  solution (1M in DCM, 1.73 mL, 1.73 mmol). The reaction was stirred at room temperature for 2 h. Sodium triacetoxyborohydride (668 mg, 3.15 mmol) was added portionwise and the reaction stirred at room temperature overnight. The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.46-1.71 (m, 6H) 1.75-2.01 (m, 4H) 3.54-3.64 (m, 1H) 3.89 (s, 3H) 4.02-4.08 (m, 1H) 6.97 (d, J 6 Hz, 1H) 7.54 (d, J=6 Hz, 1H) 
     MS ES + : 241 
     
       
         
         
             
             
         
       
     
     Intermediate 31 4-(benzyloxy)-6-chloro-N-cyclohexylpyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to a mixture of cyclohexanone (CAS 108-94-1; 2.68 g, 27.3 mmol) and 4-(benzyloxy)-6-chloropyrimidin-5-amine (Intermediate 26; 3.22 g, 13.66 mmol) in DCM (50 mL) at 0° C. under N 2  was added dropwise TiCl 4  solution (1M in DCM, 15 mL, 15 mmol) dropwise. The reaction was allowed to stir at room temperature for 2 h and then cooled to 0° C. Sodium triacetoxyborohydride (5.79 g, 27.3 mmol) was added portionwise and then the reaction allowed to stir at room temperature overnight. The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 318 
     Intermediate 32 7-(benzenesulfonyl)-4-(benzyloxy)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 1.96 g, 100.8 mmol) in DME (3 mL) at 0° C. was added NaH, 60% dispersion in oil (866 mg, 21.7 mmol). After to minutes the resulting suspension was added to a degassed solution of Pd(Ph 3 P) 4  (313 mg, 0.27 mmol) and Pd(amphos) 2 Cl 2  (192 mg, 0.271 mmol) in DME (2 mL). The resulting suspension was allowed to stir at room temperature for 20 minutes. 4-(benzyloxy)-6-chloro-N-cyclohexylpyrimidin-5-amine (Intermediate 31; 3.44 g, 10.8 mmol) was then added and the reaction mixture subjected to microwave irradiation at 120° C. for 2 h. The reaction mixture was poured into water and extracted with ethyl acetate. The organics were dried over MgSO 4  and concentrated. The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 346 
     Intermediate 33 6-amino-5-cyclohexyl-7-(phenylsulfonyl)-3H-pyrrolo[3,2-d]pyrimidin-4(5H)-one 
     
       
         
         
             
             
         
       
     
     A suspension of 7-(benzenesulfonyl)-4-(benzyloxy)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Intermediate 32; 2.6 g, 5.62 mmol) and Pd/C (598 mg, 0.562 mmol) in MeOH (20 mL) was stirred under an atmosphere of hydrogen overnight. The reaction mixture was filtered through a pad of celite and the resulting filtrate concentrated. The crude product was purified by column chromatography (silica, o-10% MeOH/DCM) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.33-1.96 (m, 9H) 2.41-2.55 (m, 2H) 7.47-7.62 (m, 4H) 7.63-7.70 (m, 2H) 7.83 (s, 1H) 8.04-8.11 (m, 2H) 
     MS ES + : 373 
     Intermediate 34 7-(benzenesulfonyl)-4-chloro-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     A solution of 6-amino-5-cyclohexyl-7-(phenylsulfonyl)-3H-pyrrolo[3,2-d]pyrimidin-4(5H)-one (Intermediate 33; 2.1 g, 5.64 mmol) in POCl 3  (8 mL, 86 mmol) was stirred at 80° C. overnight. The reaction mixture was allowed to cool and concentrated in vacuo. The crude residue was taken up in DCM and washed with water. The organics were separated and concentrated. The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.33-1.44 (m, 3H) 1.58-1.65 (m, 1H) 1.76-1.91 (m, 4H) 2.25-2.38 (m, 2H) 4.83-4.99 (m, 1H) 7.51-7.68 (m, 5H) 8.04-8.11 (m, 2H) 8.42 (s, 1H) 
     MS ES + : 391 
     
       
         
         
             
             
         
       
     
     Intermediate 35 4-chloro-N-cyclohexyl-6-methoxy-2-methylpyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to a stirred solution of cyclohexanone (CAS 108-94-1; 565 mg, 5.76 mmol) and 4-chloro-6-methoxy-2-methylpyrimidin-5-amine (CAS 88474-31-1; 500 mg, 2.88 mmol) in DCM (10 mL) at 0° C. under N 2  was added TiCl 4  solution (1M in DCM, 3.17 ml, 3.17 mmol) dropwise. The reaction was allowed to stir at room temperature for 2 h and then cooled to 0° C. Sodium triacetoxyborohydride (1.22 g, 5.76 mmol) was added portionwise and then the reaction allowed to stir at room temperature overnight. The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.11-1.30 (m, 4H) 1.49-1.57 (m, 1H) 1.62-1.69 (m, 2H) 1.72-1.81 (m, 3H) 2.40 (s, 3H) 3.39-3.49 (m, 1H) 3.88-3.96 (m, 4H) 
     MS ES + : 256 
     
       
         
         
             
             
         
       
     
     Intermediate 36 4-chloro-6-methoxy-N-(tetrahydro-2H-pyran-4-yl)pyrimidin-5-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17), to a stirred solution of 4-chloro-6-methoxypyrimidin-5-amine (CAS 15846-19-2; 0.572 mL, 6.19 mmol) and oxan-4-one (CAS 29943-42-8; 0.33 mL, 3.76 mmol) in anhydrous DCM (6 mL) under an atmosphere of nitrogen at 0° C. was added dropwise TiCl 4  solution (1M in DCM, 3.41 mL, 3.41 mmol). The reaction was stirred at room temperature for 1 h. Sodium triacetoxyborohydride (1.31 g, 6.19 mmol) was added portionwise and the reaction stirred at room temperature over a weekend. The crude product was purified by column chromatography (silica, 50-100% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.40-1.53 (m, 2H) 1.69-1.77 (m, 2H) 3.26-3.35 (m, &gt;2H due to overlap with water peak) 3.68-3.79 (m, 1H) 3.79-3.87 (m, 2H) 3.98 (s, 3H) 4.38 (d, J=10 Hz, 1H) 8.10 (s, 1H) 
     MS ES + : 244 
     
       
         
         
             
             
         
       
     
     Intermediate 37 6-amino-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide 
     
       
         
         
             
             
         
       
     
     A mixture of 2,3-dichloropyrazine (CAS 4858-85-9; 10 g, 67.1 mmol), cesium carbonate (24 g, 73.8 mmol) and malononitrile (CAS 109-77-3; 4.88 g, 73.8 mmol) in DMSO (150 mL) was stirred at 125° C. for 90 minutes then allowed to cool to rt. Cyclohexanamine (CAS 108-91-8; 150 mL, 1.31 mol) was added and the reaction mixture was stirred at 130° C. for 4 days. After cooling to rt, 2M sodium hydroxide solution (200 mL; 0.4 mol) was added and the mixture was stirred at 115° C. for 24 hr. After cooling the mixture was diluted with water and extracted with EtOAc (×3). The combined organic extracts were washed with brine, dried over MgSO 4  and concentrated. The crude product was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.20-1.33 (m, 1H) 1.35-1.47 (m, 2H) 1.64-1.78 (m, 3H) 1.81-1.89 (m, 2H) 2.37-2.49 (m, 2H) 4.32-4.44 (m, 1H) 7.08 (br. s., 1H) 7.42 (br. s., 1H) 7.77-7.89 (m, 3H) 8.04 (d, J=3 Hz, 1H) 
     MS ES + : 260. 
     Intermediate 38 5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine formate 
     
       
         
         
             
             
         
       
     
     A solution of 6-amino-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazine-7-carboxamide (Intermediate 37; 13.9 g, 53.6 mmol) in 50% aqueous sulfuric acid (100 mL) was heated at 100° C. for 2 h. The reaction mixture was allowed to cool to rt then poured into water and then basified to pH to with 2M NaOH. The resulting mixture was extracted with DCM (×3) and the organic extracts were concentrated in vacuo. The crude product was purified by column chromatography (C18-silica 5-95% methanol/water+0.1% formic acid) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.25-1.46 (m, 3H) 1.64-1.73 (m, 3H) 1.80-1.89 (m, 2H) 2.42-2.54 (m, 2H) 4.21-4.32 (m, 1H) 5.34 (s, 1H) 6.48 (br. s., 2H) 7.61 (d, J=3 Hz, 1H) 7.86 (d, J=3 Hz, 1H) 8.16 (s, 1H) 
     MS ES + : 217 
     Intermediate 3 2-{5-cyclohexyl-5-pyrrolo[2,3-b]pyrazin-6-yl}-2,3-dihydro-1H-isoindole-1,3-dione 
     
       
         
         
             
             
         
       
     
     A solution of 5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine formate (Intermediate 38; 5 g, 19.1 mmol) in DCM (0 mL) was treated with triethylamine (12.9 mL, 92 mmol) followed by phthaloyl dichloride (CAS 88-95-9; 4.93 g, 24.3 mmol). The reaction mixture was allowed to stir at rt for 3 hours then poured into water and extracted with DCM. The organic phase was separated and concentrated to yield the title compound, which was used without further purification. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.00-1.09 (m, 2H) 1.16-1.41 (m, 3H) 1.58-1.65 (m, 1H) 1.73-1.86 (m, 4H) 4.22-4.32 (m, 1H) 6.84 (s, 1H) 7.96-8.02 (m, 2H) 8.04-8.10 (m, 2H) 8.37-8.41 (m, 1H) 8.48-8.54 (m, 1H) 
     Intermediate 40 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonic add 
     
       
         
         
             
             
         
       
     
     A solution of 2-{5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-yl}-2,3-dihydro-1H-isoindole-1,3-dione (Intermediate 39; 8.63 g, 24.9 mmol) and acetic anhydride (23.5 mL, 249 mmol) in dichloromethane (100 mL) was cooled to 0° C. then sulfuric acid (6.64 mL, 125 mmol) was added dropwise. After 2 h the reaction mixture was diluted with water and extracted with DCM. The organic phase was concentrated and then azeotroped with toluene to yield the title compound. 
     MS ES + : 427. 
     Intermediate 41 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl chloride 
     
       
         
         
             
             
         
       
     
     A solution of 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonic acid (Intermediate 40; 10.63 g, 24.9 mmol) in phosphorus oxychloride (50 mL, 536 mmol) was treated with phosphorus pentachloride (5.42 g, 26.0 mmol) and heated to 80° C. for 1.5 h. The reaction mixture was slowly quenched into warm water. The aqueous mixture was allowed to cool to rt and extracted with DCM. The organic phase was concentrated to yield the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.18-1.49 (m, 3H) 1.63-1.68 (m, 1H) 1.75-1.93 (m, 4H) 2.53-2.64 (m, 2H) 4.81 (s, 1H) 8.04-8.09 (m, 2H) 8.13-8.19 (m, 2H) 8.78 (d, J=2.27 Hz, 1H) 8.90 (d, J=2.53 Hz, 1H) 
     MS ES + : 445 
     Intermediate 42 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-N-phenyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl chloride (Intermediate 41; 100 mg, 0.225 mmol) in THF (1 mL) was added DMAP (28 mg, 0.225 mmol) and aniline (CAS 62-53-3; 42 mg, 0.450 mmol) and the reaction mixture allowed to stir at room temperature overnight. The reaction mixture was diluted with water and extracted with DCM. The organics were separated and concentrated. The crude material was purified by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound.  1 H NMR (40 MHz, DMSO-d 6 ) δ ppm 1.19-1.28 (m, 1H) 1.30-1.47 (m, 2H) 1.56-1.69 (m, 1H) 1.72-1.81 (m, 3H) 2.40-2.48 (m, 3H) 4.52-4.65 (m, 1H) 6.84-6.93 (m, 1H) 6.96-6.70 (m, 2H) 7.06-7.12 (m, 2H) 8.01-8.10 (m, 2H) 8.11-8.17 (m, 2H) 8.57 (d, J=3 Hz, 1H) 8.69 (d, J=3 Hz, 1H) 10.67 (s, 1H) 
     MS ES + : 502 
     Intermediate 43 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-N-(pyridin-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl chloride (Intermediate 41; 1 mg, 0.225 mmol) in THF (1 mL) was added DMAP (28 mg, 0.225 mmol) and pyridin-3-amine (CAS 462-08-8; 42 mg, 0.450 mmol). The reaction mixture was allowed to stir at room temperature overnight. The reaction mixture was diluted with water and extracted with DCM. The crude material was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
     MS ES + : 503 
     
       
         
         
             
             
         
       
     
     Intermediate 44 2-(5-cyclohexyl-7-((4-methoxyphenyl)sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-yl)isoindoline-1,3-dione 
     
       
         
         
             
             
         
       
     
     A mixture of silver trifluoromethanesulfonate (45 mg, 0.173 mmol), 4-methoxybenzene-1-sulfonyl chloride (36 mg, 0.173 mmol) and 2-{5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-yl}-2,3-dihydro-1H-isoindole-1,3-dione (Intermediate 39; 30 mg, 0.087 mmol) in nitrobenzene (0.5 mL) was subjected to microwave heating to 120° C. for 40 minutes. The reaction mixture was partitioned between water and DCM then the organic phase was concentrated in vacuo and the residue was purified by column chromatography on silica (silica, 5-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (4 MHz, DMSO-d 6 ) δ ppm 1.22-1.32 (m, 2H) 1.32-1.46 (m, 2H) 1.60-1.67 (m, 1H) 1.70-1.85 (m, 5H) 3.81 (s, 3H) 4.63-4.74 (m, 1H) 7.08-7.16 (m, 2H) 7.89-7.96 (m, 1H) 8.04-8.10 (m, 1H) 8.14-8.21 (m, 2H) 8.60 (d, J=2 Hz, 1H) 8.72 (d, J=2 Hz, 1H) 
     MS ES + : 517. 
     Intermediates 45 to 54 were prepared by analogous methods and the data are given in Table 1. Where reactions failed to proceed to completion, further sulfonyl chloride was added and the temperature was increased (up to 150° C.) as required. Conventional heating in a sealed tube could also be employed. 
     
       
         
           
               
               
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                   
                   
                   
                   
                 Column 
                   
               
               
                   
                   
                   
                   
                 chromatography 
                 MS 
               
               
                 Intermediate 
                 Name of compound 
                 Structure 
                 Sulfonyl chloride 
                 gradient 
                 ES+ 
               
               
                   
               
             
            
               
                 45 
                 2-(5-cyclohexyl-7- (cyclopropylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Cyclopropanesulfonyl  chloride (CAS 139631-62-2) 
                 5-50%  EtOAc/petrol 
                 451 
               
               
                   
               
               
                 46 
                 2-(5-cyclohexyl-7-((3- fluorophenyl)sulfonyl)- 5H-pyrrolo[2,3-b]pyrazin- 6-yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-fluorobenzene-1- sulfonyl chloride (CAS 701-27-9) 
                 5-50%  EtOAc/petrol 
                 505 
               
               
                   
               
               
                 47 
                 2-(5-cyclohexyl-7-((2- fluorophenyl)sulfonyl)- 5H-pyrrolo[2,3-b]pyrazin- 6-yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-fluorobenzene-1- sulfonyl chloride (CAS 2905-21-7) 
                 5-50%  EtOAc/petrol 
                 505 
               
               
                   
               
               
                 48 
                 2-(5-cyclohexyl-7-((3- methoxyphenyl)sulfonyl)- 5H-pyrrolo[2,3-b]pyrazin- 6-yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-methoxybenzene-1- sulfonyl chloride (CAS 10130-74-2) 
                 5-50%  EtOAc/petrol 
                 517 
               
               
                   
               
               
                 49 
                 4-((5-cyclohexyl-6-(1,3- dioxoisoindolin-2-yl)-5H- pyrrolo[2,3-b]pyrazin-7- yl)sulfonyl)benzonitrile 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-cyanobenzene-1- sulfonyl chloride (CAS 49584-26-1) 
                 5-50%  EtOAc/petrol 
                 512 
               
               
                   
               
               
                 50 
                 2-(7-((3-chloro-4- methoxyphenyl)sulfonyl)- 5-cyclohexyl-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-chloro-4- methoxybenzene-1- sulfonyl chloride (CAS 22952-43-8) 
                 5-70%  EtOAc/petrol 
                 551 
               
               
                   
               
               
                 51 
                 2-(5-cyclohexyl-7-((6- methoxypridin-3- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 6-methoxypyridine-3- sulfonyl chloride (CAS 312300-42-8) 
                 5-75%  EtOAc/petrol 
                 518 
               
               
                   
               
               
                 52 
                 2-(5-cyclohexyl-7-((4- (trifluoromethoxy)phenyl) sulfonyl)-5H-pyrrolo[2,3- b]pyrazin-6- yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-(trifluoromethoxy)  benzenesulfonyl  chloride (CAS 94108-56-2) 
                 5-80%  EtOAc/petrol 
                 571 
               
               
                   
               
               
                 53 
                 2-(5-cyclohexyl-7-((2,3- ((2,3-dihydrobenzo[b][1,4]  dioxin-6-yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2,3-dihydrobenzo[b]  [1,4]dioxine-6- sulfonyl chloride (CAS 63758-12-3) 
                 5-40%  EtOAc/petrol 
                 545 
               
               
                   
               
               
                 54 
                 2-(5-cyclohexyl-7-((4- (difluoromethoxy)phenyl)  sulfonyl)-5H-pyrrolo[2,3- b]pyrazin-6- yl)isoindoline-1,3-dione 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-(difluoromethoxy)  benzene-1-sulfonyl  chloride (CAS 351003-34-4) 
                 5-40%  EtOAc/petrol 
                 553 
               
               
                   
               
            
           
         
       
     
     2. Final Compounds 
     Example 1 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2-(benzenesulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile (Intermediate 1; 50 g, 170 mmol) and cyclohexanamine (CAS 108-91-8; 97 mL, 850-mmol) in DMSO (100 mL) was added triethylamine (26 mL, 190 mmol). The reaction was heated thermally at 170° C. for 48 h. More cyclohexanamine (97 mL, 850 mmol) and triethylamine (26 mL, 190 mmol) were added and the reaction heated thermally at 185° C. for 24 h. The reaction was allowed to cool and diluted with brine. The resulting mixture was extracted with ethyl acetate and the organics washed with water and then with water/brine (1:1). The organics were dried (MgSO 4 ) and concentrated in vacuo. The crude product was loaded onto a plug of silica (10 g) and eluted using 0-50% EtOAc/petroleum ether. Product fractions were concentrated and this purification process repeated another 3 times. The product fractions were concentrated. The resulting residue was recrystallised from hot ethanol to afford the title compound. 
       1 H NMR (40 MHz, DMSO-d 6 ) δ ppm 1.20-1.29 (m, 1H) 1.33-1.48 (m, 2H) 1.62-1.76 (m, 3H) 1.77-1.88 (m, 2H) 2.39-2.48 (m, 2H) 4.33-4.47 (m, 1H) 7.52-7.64 (m, 5H) 7.86-7.91 (m, 1H) 8.01-8.07 (m, 2H) 8.07-8.12 (m, 1H) 
     MS ES + : 357 
     Example 2 5-cycloheptyl-7-[(4-methylbenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     A neat mixture of 2-(3-chloropyrazin-2-yl)-2-(4-methylbenzenesulfonyl)acetonitrile (Intermediate 2; 109 mg, 0.35 mmol) and cycloheptanamine (CAS 5452-35-7; 1.13 mL, 8.85 mmol) was heated in a microwave at 170° C. for 1 h and 45 mins. The reaction mixture was evaporated and purified by column chromatography (preparative HPLC, 40-80% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.40-1.75 (m, 8H), 2.32 (s, 3H), 2.35-2.47 (m, 2H) 2.95-3.07 (m, 2H) 4.45-4.60 (br. m., 1H) 7.33 (d, J=8 Hz, 2H) 7.54 (br. s., 2H) 7.82 (d, J=3 Hz, 1H) 7.91 (d, J=8 Hz, 2H) 8, (d, J=3 Hz, 1H) 
     MS ES + : 385 
     Example 3 5-cycloheptyl-7-[(4-methylbenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for 5-cycloheptyl-7-[(4-methylbenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine (Example 2), a neat mixture of 2-(3-chloropyrazin-2-yl)-2-(4-methylbenzenesulfonyl)acetonitrile (Intermediate 2; 109 mg, 0.35 mmol) and cyclohexanamine (CAS 108-91-8; 1.01 mL, 8.85 mmol) was heated in a microwave at 170° C. for 1 h and 45 mins. The reaction mixture was evaporated and purified by column chromatography (preparative HPLC, 30-70% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.15-1.31 (m, 1H) 1.32-1.48 (m, 2H) 1.60-1.76 (m, 3H) 1.77-1.87 (m, 2H) 2.33 (s, 3H) 2.37-2.48 (m, 2H) 4.32-4.44 (m, 1H) 7.35 (d, J=8 Hz, 2H) 7.57 (s, 2H) 7.88 (d, J=3 Hz, 1H) 7.92 (d, J=8 Hz, 2H) 8.08 (d, J=3 Hz, 1H) 
     MS ES + : 371 
     Example 4 5-cyclopentyl-7-[(4-methylbenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for 5-cycloheptyl-7-[(4-methylbenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine (Example 2), a neat mixture of 2-(3-chloropyrazin-2-yl)-2-(4-methylbenzenesulfonyl)acetonitrile (Intermediate 2; 109 mg, 0.35 mmol) and cyclopentanamine (CAS 1003-03-8; 0.873 mL, 8.85 mmol) was heated in a microwave at 170° C. for 1 h and 45 mins. The reaction mixture was evaporated and purified by column chromatography (preparative HPLC, 30-70% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.69-1.85 (m, 2H) 1.96-2.16 (m, 4H) 2.21-2.35 (m, 2H) 2.40 (s, 3H) 4.80-4.92 (m, 1H) 6.08 (br. &amp;, 2H) 7.27-7.33 (m, 2H) 7.92 (d, J=3 Hz, 1H) 8.10 (d, J=8 Hz, 2H) 8.26 (d, J=3 Hz, 1H) 
     MS ES + : 357 
     Example 5 7-[(4-chlorobenzene)sulfonyl]-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     A stirred solution of 2-(4-chlorophenylsulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile (Intermediate 3; 218 mg 0.664 mmol) and cyclohexanamine (CAS 108-91-8; 228 μL, 1.99 mmol) in N-methyl-2-pyrrolidinone (1.3 mL) was heated in a microwave at 170° C. for 2 h. More cyclohexanamine (228 μL, 1.99 mmol) was then added and the reaction was heated in a microwave at 170° C. for 2 h. The reaction mixture was diluted with EtOAc, washed with brine and water, dried (H frit) and evaporated to dryness. The crude product was purified by column chromatography (silica, 0-30% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.24-1.54 (m, 3H) 1.74-1.83 (m, 1H) 1.84-1.93 (m, 2H) 1.93-2.01 (m, 2H) 2.29-2.46 (m, 2H) 4.17-4.33 (m, 1H) 6.14 (br. s., 2H) 7.46 (d, J=9 Hz, 2H) 7.95 (d, J=3 Hz, 1H) 8.16 (d, J=9 Hz, 2H) 8.25 (d, J=3 Hz, 1H) 
     MS ES + : 391 
     Example 6 5-cyclohexyl-7-[(4-fluorobenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     A stirred solution of 2-(3-chloropyrazin-2-yl)-2-(4-fluorophenylsulfonyl)acetonitrile (Intermediate 4; 101 mg, 0.324 mmol) and cyclohexanamine (CAS 108-91-8; 111 μL, 0.972 mmol) in N-methyl-2-pyrrolidinone (650 μL) was heated in a microwave at 170° C. for 2 h. More cyclohexanamine (200 μL, 1.75 mmol) was added and the reaction heated in a microwave at 170° C. for 2 h. The reaction mixture was diluted with EtOAc, washed with brine and water, dried (H fit) and evaporated to dryness. The crude product was purified by column chromatography (silica, 0-30% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, METHANOL-d 4 ) δ ppm 1.26-1.57 (m, 3H) 1.69-1.83 (m, 3H) 1.86-1.98 (m, 2H) 2.48-2.64 (m, 2H) 4.25-4.38 (m, 1H) 7.19-7.27 (m, 2H) 7.90 (d, J=3 Hz, 1H) 8.03 (d, J=3 Hz, 1H) 8.10-8.18 (m, 2H) 
     MS ES + : 375 
     Example 7 5-cyclohexyl-7-{[4-(propan-2-yloxy)benzene]sulfonyl}-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     A stirred solution of 2-(3-chloropyrazin-2-yl)-2-(4-isopropoxyphenylsulfonyl)acetonitrile (Intermediate 5; 204 mg, 0.580 mmol) and cyclohexanamine (CAS 108-91-8; 199 μL, 1.74 mmol) in N-methyl-2-pyrrolidinone (1.1 mL) was heated in a microwave at 170° C. for 2 h. More cyclohexanamine (200 μL, 1.75 mmol) was added and the reaction heated in a microwave at 170° C. for 2 h. The reaction mixture was diluted with EtOAc, washed with brine and water, dried (H frit) and evaporated to dryness. The crude product was purified by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.25 (d, J=6 Hz, 6H) 1.32-1.48 (m, 2H) 1.62-1.76 (m, 3H) 1.77-1.87 (m, 2H) 2.36-2.49 (m, 3H) 4.32-4.44 (m, 1H) 4.62-4.73 (m, 1H) 7.03 (d, J=9 Hz, 2H) 7.54 (br. s, 2H) 7.88 (d, J=3 Hz, 1H) 7.94 (d, J=9 Hz, 2H) 8.08 (d, J=3 Hz, 1H) 
     MS ES + : 415 
     Example 8 5-cyclohexyl-7-(thiophene-2-sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     A stirred solution of 2-(3-chloropyrazin-2-yl)-2-(thiophen-2-ylsulfonyl)acetonitrile (Intermediate 6; 74 mg, 0.247 mmol) and cyclohexanamine (CAS 108-91-8; 282 μl, 2.47 mmol) in DMSO (120 μL) was heated in a microwave at 170° C. for 2.5 h. The reaction mixture was diluted with DMSO and purified by column chromatography (preparative HPLC, 30-70% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (4 MHz, METHANOL-d 4 ) δ ppm 1.29-1.59 (m, 3H) 1.71-1.86 (m, 3H) 1.90-1.99 (m, 2H) 2.52-2.67 (m, 2H) 4.28-4.40 (m, 1H) 7.06-7.11 (m, 1H) 7.68-7.73 (m, 1H) 7.81-7.85 (m, 1H) 7.93 (d, J=3 Hz, 1H) 8.06 (d, J=3 Hz, 1H) 
     MS ES + : 363 
     Example 9 3-(benzenesulfonyl)-1-cyclohexyl-1H-[3,2-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 555 mg, 3.07 mmol) in DME (3 mL) at 0° C. under a flow of N 2  was added sodium hydride (60% dispersion in oil, 223 mg, 5.57 mmol) and the resulting suspension allowed to stir for to minutes. In a separate flask Pd(Ph 3 P) 4  (CAS 014221-01-3; 161 mg, 0.139 mmol) in DME (3 mL) was degassed with N 2 . The suspension of pre-formed sodium salt of 2-(benzenesulfonyl)acetonitrile was added to the second vessel. After stirring for a further 10 minutes 2-bromo-N-cyclohexylpyridin-3-amine (Intermediate 7; 711 mg, 2.79 mmol) was added and the reaction mixture subjected to microwave irradiation at 120° C. for 1.5 h. The reaction mixture was poured into water and extracted with ethyl acetate and then the organics washed with brine. The organics were dried over MgSO 4  and concentrated in vacuo. The crude product was purified by column chromatography (silica, 0-500% EtOAc/DCM) to afford crude product. The crude product was triturated with hot IPA and then filtered and dried to afford the title compound. 
       1 H NMR (400 MHz, DICHLOROMETHANE-d 2 ) δ ppm 1.13-1.37 (m, 1H) 1.38-1.54 (m, 2H) 1.73-1.85 (m, 1H) 1.86-2.16 (m, 6H) 3.91-4.04 (m, 1H) 5.88 (br. s., 2H) 6.89-6.98 (m, 1H) 7.40-7.59 (m, 4H) 8.13-8.20 (m, 2H) 8.22-8.30 (m, 1H) 
     MS ES + : 356 
     Example 10 1-cyclopentyl-3-[(4-methylbenzene)sulfonyl]-1H-pyrrolo[3,2-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 3-(4-methylbenzenesulfonyl)-1H-pyrrolo[3,2-b]pyridin-2-amine (Intermediate 10; 250 mg, 0.7 mmol) in DMF (10 mL) was added DBU (264 mg, 1.4 mmol) and cyclopentyl bromide (194 mg, 1.0 mmol). The reaction was heated in a sealed tube at 80° C. The reaction mixture was poured into water and extracted with ethyl acetate. The organics were dried over Na 2 SO 4  and concentrated in vacuo. The crude product was purified by column chromatography (preparative HPLC, 5-95% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.61-1.71 (m, 2H) 1.90-2.02 (m, 6H) 2.32 (s, 3H) 4.84-4.92 (m, 1H) 6.87-6.94 (m, 1H) 7.13 (s, 2H) 7.33 (d, J=8 Hz, 2H) 7.48-7.55 (m, 1H) 7.95 (d, J=8 Hz, 2H) 8.11-8.18 (m, 1H) 
     MS ES + : 356 
     Example 11 1-cyclohexyl-3-[(4-methylbenzene)sulfonyl]-1H-pyrrolo[2,3-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     A stirred solution of 2-(2-chloropyridin-3-yl)-2-(4-methylbenzenesulfonyl)acetonitrile (Intermediate 11; 600 mg, 2.0 mmol), triethylamine (500 mg, 4.9 mmol) and cyclohexanamine (CAS 108-91-8; 2.43 g, 24.5 mmol) in DMSO (5 mL) was heated to 160° C. for 3 hours in a microwave. The reaction was poured onto ice and extracted with ethyl acetate. The organic phase was concentrated in vacuo. The resulting residue was purified by column chromatography (preparative HPLC, 5-95% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.20-1.33 (m, 2H) 1.34-1.48 (m, 3H) 1.60-1.71 (m, 3H) 1.78-1.87 (m, 2H) 2.33 (s, 3H) 4.29-4.40 (m, 1H) 6.96-7.09 (m, 3H) 7.32-7.36 (m, 2H) 7.70-7.74 (m, 1H) 7.80-7.85 (m, 2H) 7.92-7.98 (m, 1H) 
     MS ES + : 370 
     Example 12 7-(cyclohexanesulfonyl)-5-cyclohexyl-5-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2,3-dichloropyrazine (CAS 4858-85-9; 1.8 g, 12.1 mmol) and 2-(cyclohexanesulfonyl)acetonitrile (CAS 797036-54-5; 2.7 g, 14.4 mmol) in DMSO (2 mL) was added DBU (1.85 g, 12.1 mmol) and the reaction heated in a microwave to 130° C. for 3 h. To the resulting solution was added triethylamine (600 mg, 59 mmol) and cyclohexanamine (CAS 108-91-8; 6 g, 60.5 mmol) and the reaction heated in a microwave to 170° C. for 3 h. The reaction was poured onto ice and extracted with ethyl acetate. The organic phase was concentrated in vacuo. The resulting residue was purified by column chromatography (preparative HPLC, 5-95% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.04-1.30 (m, 4H) 1.33-1.49 (m, 4H) 1.55-1.63 (m, 1H) 1.66-1.80 (m, 5H) 1.80-1.98 (m, 4H) 2.39-2.49 (m, 2H) 3.09-3.24 (m, 1H) 4.32-4.44 (m, 1H) 7.31-7.43 (m, 2H) 7.91 (d, J=3 Hz, 1H) 8.09 (d, J=3 Hz, 1H) 
     MS ES + : 363 
     Example 13 5-(4,4-difluorocyclohexyl)-7-[(4-methoxybenzene)sulfonyl]-5H-pyrrolo[2,3-b]pyrazin-6-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2-(3-chloropyrazin-2-yl)-2-((4-methoxyphenyl)sulfonyl)acetonitrile (Intermediate 12; 136 mg, 0.420 mmol) and 4,4-difluorocyclohexanamine hydrochloride (CAS 675112-70-6; 433 mg, 2.52 mmol) in N-methyl-2-pyrrolidinone (2 mL) was added triethylamine (0.410 mL, 2.94 mmol). The reaction was then heated in a microwave to 180° C. for 2 h. The reaction mixture was partitioned between water and EtOAc. The phases were separated and the aqueous extracted with EtOAc. The combined organic extracts were then washed with water, dilute citric acid, water, sat. NaHCO 3 , sat. brine, dried (H-frit) and evaporated. The crude material was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DICHLOROMETHANE-d 2 ) δ ppm 1.87-2.11 (m, 4H) 2.24-2.40 (m, 2H) 2.75-2.93 (m, 2H) 3.86 (s, 3H) 4.29-4.44 (m, 1H) 6.20 (br. s., 2H) 6.99 (d, J=9 Hz, 2H) 7.96 (d, J=3 Hz, 1H) 8.11 (d, J=9 Hz, 2H) 8.22 (d, J=3 Hz, 1H) 
     MS ES + : 423 
     Example 14 1-(4,4-difluorocyclohexyl)-3-[(4-methoxybenzene)sulfonyl]-1H-pyrrolo[2,3-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2-(2-chloropyridin-3-yl)-2-((4-methoxyphenyl)sulfonyl)acetonitrile (Intermediate 13; 210 mg, 0.651 mmol) in N-methyl-2-pyrrolidinone (1 mL) was added a solution of 4,4-difluorocyclohexanamine hydrochloride (CAS 675112-70-6; 670 mg, 3.90 mmol) and triethylamine (0.635 mL, 4.55 mmol) in N-methyl-2-pyrrolidinone (2 mL) and the resulting mixture heated at 165-175° C. for 20 h. The reaction mixture was partitioned between ethyl acetate and water. The phases were separated and the aqueous extracted with ethyl acetate. The combined organics were washed with dilute citric acid, water, sat. aq. sodium bicarbonate solution and brine, dried over MgSO 4 , filtered and concentrated in vacuo. The crude product was purified by column chromatography (silica, 0-40% EtOAc/petroleum ether). Further purification was performed by column chromatography (preparative HPLC, 40-80% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (4 MHz, CHLOROFORM-d) δ ppm 1.81-2.16 (m, 4H) 2.21-2.49 (m, 2H) 2.53-2.89 (m, 2H) 3.84 (8, 3H) 4.56-4.92 (m, 1H) 5.68 (br. s., 2H) 6.86-7.14 (m, 3H) 7.77-7.99 (m, 3H) 8.00-8.15 (m, 1H) 
     MS ES + : 422 
     Example 15 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2-(2-chloropyridin-3-yl)-2-(phenylsulfonyl)acetonitrile (Intermediate 14; 100 mg, 0.342 mmol) in N-methyl-2-pyrrolidinone (1 mL) was added a solution of cyclohexanamine (CAS 108-91-8; 0.234 mL, 2.05 mmol) and triethylamine (0.048 mL, 0.342 mmol) in N-methyl-2-pyrrolidinone (1 mL) and the resulting mixture heated at 170° C. for 5 h using a microwave reactor. The reaction mixture was partitioned between ethyl acetate and water. The phases were separated and the aqueous extracted with ethyl acetate. The combined organics were washed with dilute citric acid, water, sat aq. sodium bicarbonate solution and brine, dried over MgSO 4 , filtered and concentrated in vacuo. The crude product was purified by column chromatography (silica, 0-40% EtOAc/petroleum ether). Further purification was performed by column chromatography (preparative HPLC, 40-80% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 0.20-1.56 (m, 3H) 1.72-2.00 (m, 5H) 2.14-2.51 (m, 2H) 4.49 (br. s., 1H) 5.70 (br. s., 2H) 6.89-7.16 (m, 1H) 7.40-7.56 (m, 3H) 7.82-7.91 (m, 1H) 7.92-8.00 (m, 2H) 8.03-8.11 (m, 1H) 
     MS ES + : 356 
     Example 16 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     A stirred solution of N-cyclohexyl-5-iodopyrimidin-4-amine (Intermediate 15; 139 mg, 0.459 mmol) and Pd(Ph 3 P) 4  (26.5 mg, 0.023 mmol) dry DME (2 mL) was degassed with N 2 . In a separate vial 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 91 mg, 0.504 mmol) was dissolved in dry DME (2 mL), degassed and cooled to 0° C. NaH, 60% dispersion in oil (36.7 mg, 0.917 mmol) was added and stirred 5 min. The solution of iodopyrimidine and Pd catalyst was then added via cannula, rinsing with further dry DME. The reaction mixture was then heated in a microwave at 110° C. for 1 h. The reaction mixture was partitioned between EtOAc and water. The aqueous phase was extracted with EtOAc. The combined organic extracts were washed with water, sat. brine, dried (H-frit) and evaporated. The crude material was then purified by column chromatography (silica, 0-40% EtOAc/petroleum ether) to afford the title compound.  1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.32-1.53 (m, 3H) 1.57-1.69 (m, 1H) 1.70-1.90 (m, 4H) 1.98-2.16 (m, 2H) 4.30-4.47 (m, 1H) 7.50-7.65 (m, 5H) 8.01-8.13 (m, 2H) 8.60 (s, 1H) 8.74 (s, 1H) 
     MS ES + : 357 
     Example 17 3-(benzenesulfonyl)-1-(4,4-difluorocyclohexyl)-1H-pyrrolo[2,3-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-b]pyridin-2-amine (Example 15), a stirred solution of 2-(2-chloropyridin-3-yl)-2-(phenylsulfonyl)acetonitrile (Intermediate 14; 239 mg, 0.816 mmol), 4,4-difluorocyclohexanamine hydrochloride (CAS 675112-70-6; 662 mg, 4.90 mmol) and triethylamine (0.8 mL, 5.71 mmol) in N-methyl-2-pyrrolidinone (2. mL) was heated at 170° C. for 5 h using a microwave reactor. The crude product was purified by column chromatography (silica, 0-40% EtOAc/petroleum ether). Further purification was performed by column chromatography (preparative HPLC, 40-80% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.82-2.14 (m, 4H) 2.17-2.46 (m, 2H) 2.58-2.87 (m, 2H) 4.49-4.90 (m, 1H) 5.76 (s, 2H) 6.97-7.14 (m, 1H) 7.39-7.63 (m, 3H) 7.76-7.93 (m, 1H) 7.97 (d, J=7 Hz, 2H) 8.04-8.14 (m, 1H) 
     MS ES + : 392 
     Example 19 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     To a stirred degassed solution of 4-chloro-N-cyclohexylpyrimidin-5-amine (Intermediate 16; 209 mg, 0.987 mmol) in dry DME (2 mL) was added Pd(Ph 3 P) 4  (29 mg, 0.025 mmol) and Pd(amphos) 2 Cl 2  (18 mg, 0.025 mmol). In a separate vial, 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 197 mg, 1.09 mmol) was dissolved in dry DME (2 mL), degassed, cooled in ice and treated with NaH, 60% dispersion in oil (79 mg, 1.98 mmol). The second vial was stirred in ice for 5 min, then at rt for 5 min, under a gentle N 2  stream. The solution of pyrimidine and Pd catalysts was then added via cannula, rinsing with further dry DME. The reaction heated in the microwave at 110° C. for 1 h. The reaction mixture was partitioned between EtOAc and water. The aqueous phase was extracted with EtOAc. The combined organic extracts were washed with water, sat. brine, dried (H-frit) and evaporated. The crude product was purified by column chromatography (silica, 50-90% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.32-1.53 (m, 3H) 1.57-1.69 (m, 1H) 1.70-1.90 (m, 4H) 1.98-2.16 (m, 2H) 4.30-4.47 (m, 1H) 7.50-7.65 (m, 5H) 8.01-8.13 (m, 2H) 8.60 (s, 1H) 8.74 (s, 1H) 
     MS ES + : 357 
     Example 20 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 330 mg, 1.82 mmol) in DME (3 mL) at 0° C. under a flow of N 2  was added NaH, 60% dispersion in oil (132 mg, 3.31 mmol) and the resulting suspension allowed to stir for 10 min. In a separate flask Pd(Ph 3 P) 4  (96 mg, 0.083 mmol) in DME (3 mL) was degassed. The solution in the first flask was added to the solution of Pd(Ph 3 P) 4  in DME. After stirring for a further to min N-cyclohexyl-4-iodopyridin-3-amine (Intermediate 17; 500 mg, 1.66 mmol) was added and the reaction mixture subjected to microwave irradiation at 120° C. for 1.5 h. The reaction mixture was poured into water and extracted with ethyl acetate and then the organics washed with brine. The organics were dried over MgSO 4  and concentrated. The crude product was purified by column chromatography (basic silica, 0-20% EtOAc/petroleum ether). The resulting solid was recrystallised from hot IPA/water to afford the title compound. 
       1 H NMR (400 MHz, DICHLOROMETHANE-d 2 ) δ ppm 1.28-1.42 (m, 1H), 1.44-1.59 (m, 2H), 1.78-1.89 (m, 1H), 1.92-2.12 (m, 4H), 2.12-2.30 (m, 2H), 3.94-4.10 (m, 1H), 5.89 (br. s., 2H), 7.45-7.62 (m, 4H), 7.89-8.07 (m, 2H), 8.17-8.22 (m, 1H), 8.68 (s, 1H) 
     MS ES + : 356 
     Example 21 3-(benzenesulfonyl)-1-(4,4-difluorocyclohexyl)-1H-pyrrolo[3,2-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-c]pyridin-2-amine (Example 20), to a stirred solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 548 mg, 3.02 mmol) in DME (3 mL) at 0° C. under a flow of N 2  was added NaH, 60% dispersion in oil (220 mg, 5.50 mmol) and the resulting suspension allowed to stir for to min. In a separate flask Pd(Ph 3 P) 4  (159 mg, 00.137 mmol) in DME (3 mL) was degassed. The solution in the first flask was added to the solution of Pd(Ph 3 P) 4  in DME. After stirring for a further to min, 2-bromo-N-(4,4-difluorocyclohexyl)pyridin-3-amine (Intermediate 18; 800 mg, 2.75 mmol) was added and the reaction mixture subjected to microwave irradiation at 120° C. for 1.5 h. The crude product was purified by column chromatography (basic silica, 0-50% DCM/EtOAc). The resulting solid was triturated with hot ethanol to afford the title compound.  1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.81-1.93 (m, 2H) 1.95-2.38 (m, 6H) 4.50-4.66 (m, 1H) 6.89-6.97 (m, 1H) 7.17 (s, 2H) 7.47-7.60 (m, 4H) 8.04-8.09 (m, 2H) 8.11-8.15 (m, 1H) 
     MS ES + : 392 
     Example 22 1-(4,4-difluorocyclohexyl)-3-[(4-methoxybenzene)sulfonyl]-1H-pyrrolo[3,2-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-c]pyridin-2-amine (Example 20), to a stirred solution of 2-((4-methoxyphenyl)sulfonyl)acetonitrile (CAS 132276-87-o; 638 mg, 3.02 mmol) in DME (4 mL) at 0° C. under a flow of N 2  was added NaH, 60% dispersion in oil (2200 mg, 5.50 mmol) and the resulting suspension allowed to stir for 10 minutes. In a different flask Pd(Ph 3 P) 4  (159 mg, 0.137 mmol) in DME (4 mL) was degassed. The solution in the first flask was added to the solution of Pd(Ph 3 P) 4  in DME. After stirring for a further to minutes 2-bromo-N-(4,4-difluorocyclohexyl)pyridin-3-amine (Intermediate 18; 800 mg, 2.75 mmol) was added and the reaction mixture subjected to microwave irradiation at 120° C. for 1.5 h. The crude product was purified by column chromatography (basic silica, 0-100% DCM/EtOAc). The resulting solid was triturated with hot ethanol to afford the title compound. 
       1 H NMR (400 MHz, DICHLOROMETHANE-d 2 ) δ ppm 1.88-2.12 (m, 4H), 2.27-2.39 (m, 2H), 2.44-2.59 (m, 2H), 3.85 (s, 3H), 4.09-4.28 (m, 1H), 5.93 (s, 2H), 6.89-7.05 (m, 3H), 7.50-7.54 (m, 1H), 8.07-8.20 (m, 2H), 8.30-8.34 (m, 1H) 
     MS ES + : 422 
     Example 23 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[3,2-c]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-c]pyridin-2-amine (Example 20), to a stirred degassed solution Pd(Ph 3 P) 4  (73 mg, 0.063 mmol) in anhydrous DME (3 mL) under an atmosphere of nitrogen was added a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 252 mg, 1.39 mmol) and NaH, 60% dispersion in oil (106 mg, 2.65 mmol) in anhydrous DME (4 mL). The resulting mixture was stirred at room temperature for 10 min followed by addition of a solution of 3-bromo-N-cyclohexylpyridin-4-amine (Intermediate 19; 322 mg, 1.262 mmol) in anhydrous DME (1 mL). The reaction mixture was heated at 120° C. for 1.5 h. Purification was carried out by column chromatography (silica, 0-100% EtOAc/petroleum ether). Further purification was performed by column chromatography (preparative HPLC, 20-600% acetonitrile/water (with 0.1% formic acid)) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.19-1.55 (m, 3H) 1.76-2.29 (m, 7H) 3.82-4.19 (m, 1H) 5.75 (br. s, 2H) 7.20 (d, J=6 Hz, 1H) 7.38-7.62 (m, 3H) 7.88-8.09 (m, 2H) 8.23 (d, J=6 Hz, 1H) 8.92 (s, 1H) 
     MS ES + : 356 
     Example 24 methyl N-[7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-yl]carbamate 
     
       
         
         
             
             
         
       
     
     To a stirred solution of methyl N-[7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[2,3-b]pyrazin-6-yl]-N-(methoxycarbonyl)carbamate (Intermediate 20; 0.214 g, 0.453 mmol) in MeOH (7 mL) was added sodium methanolate (16 mg, 0.3 mmol) and the resulting mixture stirred at room temperature for 3 h. A further portion of sodium methoxide (10 mg, 0.19 mmol) was added and the reaction was stirred at room temperature for a further 2 h. The solvent was removed under reduced pressure. The residue was partitioned between DCM and water, passed through a phase separator and concentrated in vacuo. Purification was performed by column chromatography (preparative HPLC, 10-50% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (4000 MHz, CHLOROFORM-d) δ ppm 1.27-1.46 (m, 3H) 1.66-2.05 (m, 5H) 2.47-2.73 (m, 2H) 3.89 (s, 3H) 4.09-4.28 (m, 1H) 7.37-7.64 (m, 3H) 8.05-8.21 (m, 2H) 8.27 (d, J=3 Hz, 2H) 8.52 (d, J=3 Hz, 1H) 
     MS ES + : 415 
     Example 25 3-(benzenesulfonyl)-1-(4,4-difluorocyclohexyl)-6-methyl-1H-pyrrolo[2,3-b]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     To a stirred solution 2-amino-1-(4,4-difluorocyclohexyl)-6-methyl-3-(phenylsulfonyl)-1H-pyrrolo[2,3-b]pyridine 7-oxide (Intermediate 22; 65 mg, 0.154 mmol) in chloroform (2 mL) under an atmosphere of nitrogen was added trichlorophosphine (0.1 mL, 1.15 mmol). The resulting mixture was heated at reflux for 1 h. The mixture was partitioned between DCM and saturated NaHCO 3 . The phases were separated and the aqueous extracted with DCM. The combined organics were washed with saturated NaHCO 3 , dried over MgSO 4  and concentrated in vacuo. The crude product was purified by column chromatography (preparative HPLC, 40-80% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.76-2.19 (m, 4H) 2.22-2.41 (m, 2H) 2.52 (s, 3H) 2.58-2.81 (m, 2H) 4.58-4.87 (m, 1H) 5.57 (br. s, 2H) 6.91 (d, J=8 Hz, 1H) 7.36-7.60 (m, 3H) 7.76 (d, J=8 Hz, 1H) 7.86-8.07 (m, 2H) 
     MS ES + : 406 
     Example 26 7-(benzenesulfonyl)-5-cyclohexyl-4-methoxy-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 19), to a stirred degassed solution of Pd(Ph 3 P) 4  (14 mg, 0.013 mmol) and Pd(amphos) 2 Cl 2  (9 mg, 0.013 mmol) in anhydrous DME (1 mL) under an atmosphere of nitrogen was added a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 100 mg, 0.550 mmol) and NaH, 60% dispersion in oil (44.0 mg, 1.100 mmol) in anhydrous DME (1 mL). The resulting mixture was stirred at room temperature for 10 min followed by addition of a solution of 4-chloro-N-cyclohexyl-6-methoxypyrimidin-5-amine (Intermediate 23; 121 mg, 0.5 mmol) in anhydrous DME (1 mL). The reaction mixture was heated at 120° C. for 1.5 h. The crude product was purified by column chromatography (preparative HPLC, 30-70% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.13-1.53 (m, 4H) 1.65-2.51 (m, 7H) 4.09 (s, 3H) 5.86 (br. s., 2H) 7.42-7.60 (m, 3H) 8.14-8.30 (m, 2H) 8.51 (s, 1H) 
     MS ES + : 387 
     Example 27 5-(benzenesulfonyl)-3-chloro-7-cyclohexyl-7H-pyrrolo[2,3-c]pyridazin-6-amine 
     
       
         
         
             
             
         
       
     
     Prepared as described for 3-(benzenesulfonyl)-1-cyclohexyl-1H-pyrrolo[2,3-c]pyridin-2-amine (Example 20), to a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 34 mg, 0.189 mmol) in DME (3 mL) at 0° C. was added NaH, 60% dispersion in oil (14 mg, 0.344 mmol). After 10 minutes the resulting suspension was added to a degassed solution of Pd(Ph 3 P) 4  (10 mg, 8.60 μmol) in DME (2 mL). The resulting suspension was allowed to stir at room temperature for 20 minutes. 4-bromo-6-chloro-N-cyclohexylpyridazin-3-amine (Intermediate 24; 50 mg, 0.172 mmol) was then added and the reaction mixture subjected to microwave irradiation at 120° C. for 2 h. Purification was carried out by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.18-1.49 (m, 3H), 1.62-1.7 (m, 3H), 1.77-1.86 (m, 2H), 2.42-2.49 (m, 2H), 4.43 (br. s., 1H), 7.47 (s, 1H), 7.55-7.70 (m, 3H), 7.96 (br. s., 2H), 8.00-8.08 (m, 2H) 
     MS ES + : 391 
     Example 28 5-(benzenesulfonyl)-7-cyclohexyl-7H-pyrrolo[2,3-c]pyridazin-6-amine 
     
       
         
         
             
             
         
       
     
     A solution of 5-(benzenesulfonyl)-3-chloro-7-cyclohexyl-7H-pyrrolo[2,3-c]pyridazin-6-amine (Example 27; 31 mg, 0.079 mmol) in THF (2 mL) was passed through an H-Cube using a 10% Palladium on carbon cat-cart at 40° C. at ‘full H 2 ’. The reactant was cycled through the H-Cube for 2 h at 1 mL/min. The product solution was then concentrated in vacuo. Purification was carried out by column chromatography (silica, 0-50% EtOAc/petroleum ether) followed by column chromatography (silica, 0-10% MeOH/DCM) and finally by trituration with diethyl ether to afford the title compound. 
       1 H NMR (400 MHz, DICHLOROMETHANE-d 2 ) δ ppm 1.25-1.44 (m, 3H), 1.62-1.71 (m, 1H), 1.75-1.98 (m, 4H), 2.34-2.48 (m, 2H), 4.32 (br. s., 1H), 6.13 (br. s, 2H), 7.31-7.55 (m, 4H), 7.78-7.89 (m, 2H), 8.57-8.67 (m, 1H) 
     MS ES + : 357 
     Example 29 7-(benzenesulfonyl)-5-(4,4-difluorocyclohexyl)-4-ethoxy-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     As described for 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 19), to a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 373 mg, 2.06 mmol) in DME (3 mL) at 0° C. was added NaH, 60% dispersion in oil (165 mg, 4.11 mmol). After 10 min the resulting suspension was added to a degassed solution of Pd(Ph 3 P) 4  (59 mg, 0.051 mmol) and Pd(amphos) 2 C 2  (36 mg, 0.051 mmol) in DME (2 mL). The resulting suspension was allowed to stir at room temperature for 20 minutes. 4-chloro-N-(4,4-difluorocyclohexyl)-6-ethoxypyrimidin-5-amine (Intermediate 25; 6 mg, 2.06 mmol) was then added and the reaction mixture subjected to microwave irradiation at 120° C. for 2 b. Purification was carried out by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.34-1.41 (m, 3H), 1.71-1.80 (m, 2H), 1.92-2.21 (m, 4H), 2.44-2.61 (m, 2H), 4.43-4.45 (m, 3H), 7.34 (br. s, 2H), 7.52-7.64 (m, 3H), 8.00-8.10 (nm, 2H), 8.30 (s, 1H) 
     MS ES + : 437 
     Example 30 7-(benzenesulfonyl)-4-(benzyloxy)-5-(4,4-difluorocyclohexyl)-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     As described for 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 19), to a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 512 mg, 2.83 mmol) in DME (3 mL) at 0° C. was added NaH, 60% dispersion in oil (226 mg, 5.65 mmol). After to min the resulting suspension was added to a degassed solution of Pd(Ph 3 P) 4  (0.082 g, 0.071 mmol) and Pd(amphos) 2 Cl 2  (0.050 g 0.071 mmol) in DME (2 mL). The resulting suspension was allowed to stir at room temperature for 20 minutes. 4-(benzyloxy)-6-chloro-N-(4,4-difluorocyclohexyl)pyrimidin-5-amine (Intermediate 27; 1 g, 2.83 mmol) was then added and the reaction mixture subjected to microwave irradiation at 120° C. for 2 h. Purification was carried out by column chromatography (silica, 0-30% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.64-1.78 (m, 2H), 0.83-2.11 (m, 4H), 2.40-2.58 (m, 2H), 4.44-4.57 (m, 1H), 5.55 (s, 2H), 7.26-7.39 (m, 5H), 7.42-7.48 (m, 2H), 7.51-7.66 (m, 3H), 8.02-8.10 (m, 2H), 8.32 (s, 1H) 
     MS ES + : 499 
     Example 31 6-amino-5-(4,4-difluorocyclohexyl)-7-(phenylsulfonyl)-5H-pyrrolo[3,2-d]pyrimidin-4-ol 
     
       
         
         
             
             
         
       
     
     A solution of 7-(benzenesulfonyl)-4-(benzyloxy)-5-(4,4-difluorocyclohexyl)-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 30; 420 mg, 0.842 mmol) in methanol (17 mL) was passed through an H-Cube using a palladium on carbon (10%) cat-cart at ‘full H 2 ’ at room temperature at 1 mL/min. The product solution was concentrated and triturated with ethyl acetate to afford the title compound. 
       1 H NMR (4 MHz, DMSO-d 6 ) δ ppm 1.61-1.71 (m, 2H), 1.85-2.18 (m, 4H), 2.68-2.83 (m, 2H), 4.32-4.52 (m, 1H), 6.94 (s, 2H), 7.51-7.68 (m, 3H), 7.86 (s, 1H), 7.94-8.12 (m, 2H), 12.04 (br. s., 1H) 
     MS ES + : 409 
     Example 32 7-(benzenesulfonyl)-4-chloro-5-(4,4-difluorocyclohexyl)-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     To a stirred suspension of 6-amino-7-(benzenesulfonyl)-5-(4,4-difluorocyclohexyl)-3H-pyrrolo[3,2-d]pyrimidin-4(5H)-one (Example 31; 75 mg, 0.184 mmol) in POCl 3  (1 mL, 10.7 mmol) was heated at 80° C. overnight. The reaction was quenched slowly into warm water. The resulting solution was basified to pH 12  with 2M NaOH. The resulting aqueous mixture was extracted with DCM. The organics were separated and concentrated. Trituration with diethyl ether afforded the title compound. 
       1 H NMR (400 MHz, DICHLOROMETHANE-d 2 ) δ ppm 1.86-2.23 (m, 4H), 2.27-2.68 (m, 4H), 5.41-5.73 (m, 1H), 6.28 (br. s., 2H), 7.35-7.73 (m, 3H), 8.05-8.32 (m, 2H), 8.56 (br. s., 1H) 
     MS ES + : 427 
     Example 33 7-(benzenesulfonyl)-5-(4,4-difluorocyclohexyl)-4-N-methyl-5H-pyrrolo[3,2-d]pyrimidine-4,6-diamine 
     
       
         
         
             
             
         
       
     
     A solution of 7-(benzenesulfonyl)-4-chloro-5-(4,4-difluorocyclohexyl)-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 32; 40 mg, 0.094 mmol) and methanamine, 2M in THF (0.234 mL, 0.469 mmol) in THF (1 mL) was subjected to microwave irradiation at 120-160° C. for a total of 7 h. The reaction mixture was concentrated in vacuo. To the crude product was added methanamine 2M in THF (2 ml). The solution was subjected to microwave irradiation for a further 2 h at 160° C. The reaction mixture was poured into sat. NaHCO 3  and extracted with DCM. The organics were separated and concentrated to afford the title compound. 
       1 H NMR (4 MHz, DMSO-d 6 ) δ ppm 1.95-2.04 (m, 2H), 2.06-2.30 (m, 4H), 2.33-2.48 (m, 2H), 2.95 (d, J=5 Hz, 3H), 4.45-4.58 (m, 1H), 5.84-5.91 (m, 1H), 6.82 (s, 2H), 7.51-7.73 (m, 3H), 8.04-8.15 (m, 2H), 8.23 (s, 1H) 
     MS ES + : 422 
     Example 34 7-(benzenesulfonyl)-5-cyclohexyl-4-N,4-N-dimethyl-5H-pyrrolo[3,2-d]pyrimidine-4,6-diamine 
     
       
         
         
             
             
         
       
     
     As described for 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 19), to a stirred degassed solution of Pd(Ph 3 P) 4  (28 mg, 0.024 mmol) and Pd(amphos) 2 Cl 2  (17 mg, 0.024 mmol) in anhydrous DME (3 mL) under an atmosphere of nitrogen was added a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 261 mg, 1.44 mmol) and NaH, 60% dispersion in oil (115 mg, 2.89 mmol) in anhydrous DME (3 mL). The resulting mixture was stirred at room temperature for to min followed by addition of a solution of 6-chloro-5-N-cyclohexyl-4-N,4-N-dimethylpyrimidine-4,5-diamine (Intermediate 28; 245 mg, 0.962 mmol) in anhydrous DME (3 mL). The reaction mixture was heated at 125° C. for 20 h. The crude product was purified by column chromatography (preparative HPLC, 40-80% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.03-2.10 (m, 10H) 2.89 (s, 6H) 4.67-4.92 (m, 1H) 6.01 (br. s., 2H) 7.39-7.63 (m, 3H) 8.11-8.32 (m, 2H) 8.53 (s, 1H) 
     MS ES + : 400 
     Example 35 7-(benzenesulfonyl)-5-cyclopentyl-4-methoxy-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     As described for 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 19), to a stirred degassed solution of Pd(Ph 3 P) 4  (32 mg, 0.027 mmol) and Pd(amphos) 2 Cl 2  (19 mg, 0.027 mmol) in anhydrous DME (2 mL) under an atmosphere of nitrogen was added a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 298 mg, 1.65 mmol) and NaH, 60% dispersion in oil (132 mg, 3.29 mmol) in anhydrous DME (2 mL). The resulting mixture was stirred at room temperature for to min followed by addition of a solution of 4-chloro-N-cyclopentyl-6-methoxypyrimidin-5-amine (Intermediate 29; 250 mg, 1.10 mmol) in anhydrous DME (2 mL). The reaction mixture was heated at 120° C. for 16 h. The crude product was purified by recrystallisation from DMSO/MeOH (1:1) to afford the title compound.  1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.46-1.73 (m, 2H) 1.80-2.05 (m, 6H) 3.98 (s, 3H) 4.70-5.01 (m, 1H) 7.25 (br. s, 2H) 7.42-7.72 (m, 3H) 7.94-8.13 (m, 2H) 8.33 (s, 1H) 
     MS ES + : 373 
     Example 36 3-(benzenesulfonyl)-1-cyclohexyl-7-methoxy-1-pyrrolo[2,3-c]pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     As described for 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 19), to a stirred degassed solution of Pd(Ph 3 P) 4  (23.64 mg, 0.0020 mmol) and Pd(amphos) 2 Cl 2  (19 mg, 0.027 mmol) in anhydrous DME (2 mL) under an atmosphere of nitrogen was added a solution of 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 148 mg, 0.818 mmol) and NaH, 60% dispersion in oil (65.5 mg, 1.637 mmol) in anhydrous DME (3 mL). The resulting mixture was stirred at room temperature for to min followed by addition of a solution of 4-chloro-N-cyclohexyl-2-methoxypyridin-3-amine (Intermediate 30; 197 mg, 0.818 mmol) in anhydrous DME (1 mL). The reaction mixture was subjected to microwave irradiation at 120° C. for 2 h. Purification was carried out by column chromatography (C18-silica 5-95% methanol/water+0.1% ammonia) to afford the title compound. 
       1 H NMR (4 MHz, DICHLOROMETHANE-d 2 ) δ ppm 1.06-1.44 (m, 4H), 1.59-2.10 (m, 6H), 2.14-2.58 (m, 1H), 3.91 (s, 3H), 5.58 (br. s., 2H), 7.10 (d, J=5 Hz, 1H), 7.32-7.46 (m, 3H), 7.63 (d, J=5 Hz, 1H), 7.79-7.87 (m, 2H) 
     MS ES + : 386 
     Example 37 6-amino-7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidine-4-carbonitrile 
     
       
         
         
             
             
         
       
     
     A stirred suspension of dicyanozinc (CAS 557-21-1; 18 mg, 0.153 mmol), Pd(Ph 3 P) 4  (30 mg, 0.026 mmol) and 7-(benzenesulfonyl)-4-chloro-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Intermediate 34; 100 mg, 0.256 mmol) in N,N-dimethylformamide (1 mL) was subjected to microwave irradiation at 150° C. for 30 minutes. The reaction mixture was poured into sat. NaHCO 3  solution and extracted with ethyl acetate. The organics were washed with brine, dried over MgSO 4  and concentrated to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.37-1.50 (m, 3H) 1.55-1.72 (m, 1H) 1.80-2.00 (m, 4H) 2.24-2.41 (m, 2H) 4.55-4.82 (m, 1H) 7.52-7.69 (m, 3H) 7.97 (br.s., 2H) 8.05-8.11 (m, 2H) 8.67 (s, 1H). 
     MS ES + : 382 
     Example 38 5-cyclohexyl-7-(2-fluorobenzenesulfonyl)-4-methoxy-2-methyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     As described for 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 19), to a solution of 2-(2-fluorobenzenesufonyl)acetonitrile (CAS 59849-52-4; 195 mg, 0.978 mmol) in DME (1 mL) was added NaH, 60% dispersion in oil (86 mg, 2.15 mmol). In a separate flask Pd(Ph 3 P) 4  (28 mg, 0.024 mmol), Pd(amphos) 2 Cl 2  (17 mg, 0.024 mmol) and 4-chloro-N-cyclohexyl-6-methoxy-2-methylpyrimidin-5-amine (Intermediate 35; 250 mg, 0.978 mmol) were stirred in DME (2 mL) and degassed. To the catalyst/substrate mixture was added the preformed sodium salt of 2-(2-fluorobenzenesulfonyl)acetonitrile and the reaction subjected to microwave irradiation at 130° C. for 2 h. Purification was carried out by column chromatography (silica, 0-10% MeOH/DCM) followed by trituration with ethyl acetate to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.21-1.31 (m, 1H) 1.36-1.49 (m, 2H) 1.63-1.70 (m, 3H) 1.79-1.88 (m, 2H) 2.12-2.24 (m, 2H) 2.35 (s, 3H) 3.97 (s, 3H) 4.28-4.48 (m, 1H) 7.17 (br. s., 2H) 7.27-7.34 (m, 1H) 7.36-7.42 (m, 1H) 7.60-7.70 (m, 1H) 8.01-8.07 (m, 1H) 
     MS ES + : 419 
     Example 39 5-cyclohexyl-7-(3-fluorobenzenesulfonyl)-4-methoxy-2-methyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     As described for 7-(benzenesulfonyl)-5-cyclohexyl-5H-pyrrolo[3,2-d]pyrimidin-6-amine (Example 19), to a solution of 2-(3-fluorobenzenesulfonyl)acetonitrile (CAS 61081-29-6; 300 mg, 1.51 mmol) in dioxane (3 mL) was added NaH, 60% dispersion in oil (133 mg, 3.31 mmol). In a separate flask Pd(Ph 3 P) 4  (70 mg, 0.060 mmol), Pd(amphos) 2 Cl 2  (43 mg, 0.060 mmol) and 4-chloro-N-cyclohexyl-6-methoxy-2-methylpyrimidin-5-amine (Intermediate 35; 385 mg, 0.978 mmol) were stirred in dioxane (2 mL) and degassed. To the catalyst/substrate mixture was added the preformed sodium salt of 2-(3-fluorobenzenesulfonyl)acetonitrile and the reaction heated at reflux for 3 h. Purification was carried out by column chromatography (C18-silica 5-95% methanol/water+0.1% ammonia). 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.20-1.30 (m, 1H) 1.33-1.47 (m, 2H) 1.58-1.70 (m, 3H) 1.77-1.84 (m, 2H) 2.08-2.21 (m, 2H) 2.49 (s, 3H) 3.99 (s, 3H) 4.25-4.46 (m, 1H) 7.19 (br. s., 2H) 7.42-7.51 (m, 1H) 7.57-7.65 (m, 1H) 7.83-8.00 (m, 2H) 
     MS ES + : 419 
     Example 40 7-(benzenesulfonyl)-4-methoxy-5-(oxan-4-yl)-5H-pyrrolo[3,2-d]pyrimidin-6-amine 
     
       
         
         
             
             
         
       
     
     A stirred solution of 4-chloro-6-methoxy-N-(oxan-4-yl)pyrimidin-5-amine (249 mg, 1.02 mmol), 2-(benzenesulfonyl)acetonitrile (CAS 7605-28-9; 204 mg, 1.12 mmol), Pd(Ph 3 P) 4  (59 mg, 0.051 mmol) and Pd(amphos) 2 Cl 2  (36 mg, 0.051 mmol) in Dioxane (5 mL) was degassed for 5 minutes. NaHMDS solution (2M in THF, 1.53 mL, 3.07 mmol) was added and the mixture was heated to reflux for 1.5 h. The mixture was partitioned between ethyl acetate and sat. aq. NaHCO 3  then the organic phase was washed with brine and dried over MgSO 4  and then concentrated in vacuo. Purification was carried out by column chromatography (silica, 0-100% EtOAc/Petrol then 0-10% MeOH/DCM). Further purification was carried out by column chromatography (preparative HPLC, 20-60% acetonitrile/water (with 0.1% ammonia)) to afford the title compound. 
       1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 1.57-1.66 (m, 2H) 2.34-2.47 (m, 2H) 3.45 (t, J=11 Hz, 2H) 3.94-4.03 (m, 5H) 4.56-4.67 (m, 1H) 7.32 (br. s, 2H) 7.51-7.62 (m, 3H) 8.02-8.08 (m, 2H) 8.32 (s, 1H) 
     MS ES + : 389 
     Example 41 6-amino-5-cyclohexyl-N-phenyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide 
     
       
         
         
             
             
         
       
     
     To a solution of 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-N-phenyl-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide (Intermediate 43; 46 mg, 0.092 mmol) in EtOH (1 mL) was added hydrazine monohydrate (13 μL, 0.275 mmol) and the reaction mixture stirred at reflux overnight. The reaction mixture was filtered and the resulting solid washed with methanol. The combined filtrates were concentrated in vacuo. Purification was carried out by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound. 
       1 H NMR (400 MHz, DICHLOROMETHANE-d 2 ) δ ppm 1.21-1.49 (m, 4H) 1.67-1.82 (m, 2H) 1.84-1.97 (m, 2H) 2.23-2.39 (m, 2H) 4.04-4.18 (m, 1H) 5.77 (br. s., 2H) 6.97-7.07 (m, 3H) 7.10-7.20 (m, 2H) 7.32 (br. s., 1H) 7.90-7.97 (m, 1H) 8.14-8.22 (m, 1H) 
     MS ES + : 372 
     Example 42 6-amino-5-cyclohexyl-N-(pyridin-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide 
     
       
         
         
             
             
         
       
     
     To a solution of 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-N-(pyridin-3-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonamide (Intermediate 43; 20 mg, 0.040 mmol) in EtOH (1 mL) was added hydrazine monohydrate (6 μL, 0.119 mmol) and the reaction mixture stirred at reflux overnight. The reaction mixture was filtered and the resulting solid washed with methanol. The combined filtrates were concentrated in vacuo. Purification was carried out by column chromatography (silica, 0-50% EtOAc/petroleum ether) to afford the title compound.  1 H NMR (4 MHz, DMSO-d 6 ) δ ppm 1.20-129 (m, 1H) 1.33-1.50 (m, 2H) 0.61-1.73 (m, 3H) 1.75-1.88 (m, 2H) 2.35-2.48 (m, 2H) 4.30-4.43 (m, 1H) 7.12-7.22 (m, 1H) 7.36-7.47 (m, 3H) 7.84-7.93 (m, 1H) 8.02-8.13 (m, 2H) 8.21-8.8 (m, 1H) 10.42 (s, 1H) 
     MS ES + : 373 
     Examples 43 to 56 (see Table 2 following) were prepared according to one of the procedures 1, 2 or 3 described below. 
     Procedure 1 
     A solution of 2-(benzenesulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile (Intermediate 1; 100 mg, 0.34 mmol) in NMP (0.5 mL) was treated with a primary amine (0.68 mmol) and heated in the microwave at 170° C. for 1 h. Where the amine was used as a hydrochloride salt, triethylamine (0.095 mL, 0.68 mmol, 2 eq.) was included in the reaction. A further portion of each amine (1.14 mmol, 3 eq) was added and heating was repeated as before. The reaction mixtures were purified directly by preparative HPLC using one of the methods listed below. 
     Procedure 2 
     A solution of 2-(benzenesulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile (Intermediate 1; 110 mg, 0.326 mmol) in DMSO (1 mL) was treated with a primary amine (1.96 mmol, 6 eq.) and triethylamine (0.045 mL, 0.326 mmol) and heated to 180° C. for 3 h. The reaction mixtures were diluted with DMSO (2 mL), filtered and purified by preparative HPLC using one of the methods listed below. 
     Procedure 3 
     A solution of 2-(benzenesulfonyl)-2-(3-chloropyrazin-2-yl)acetonitrile (Intermediate 1; 70 mg, 0.238 mmol) in NMP (1.0 mL) was treated with a primary amine (1.43 mmol) and triethylamine (0.033 mL, 0.238 mmol) and heated in the microwave at 180° C. for 2.5 h. Where the amine used was a hydrochloride salt, triethylamine (0.196 mmol, 1.43 mmol) was included in the reaction. Samples were typically diluted with DMSO, filtered and purified by preparative HPLC using one of the methods listed below. If an aqueous workup was necessary, the reaction mixture was diluted with water and extracted with EtOAc). The combined extracts were washed with citric acid solution, water, sodium bicarbonate solution, water and brine then dried (H-frit) and evaporated, with the crude product then being purified by preparative HPLC using one of the methods listed below. 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 HPLC  
                 Gradient (acetonitrile/water  
               
               
                   
                 Method 
                 (with 0.1% ammonia)) 
               
               
                   
                   
               
             
            
               
                   
                 A 
                  5-25% 
               
               
                   
                 B 
                  5-40% 
               
               
                   
                 C 
                 10-50% 
               
               
                   
                 D 
                 20-60% 
               
               
                   
                 E 
                 30-70% 
               
               
                   
                 F 
                 40-80% 
               
               
                   
                 G 
                 55-95% 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                   
                   
                   
                   
                 Syn- 
                 Purifi- 
                   
                   
               
               
                   
                   
                   
                   
                 thesis 
                 cation 
                   
                   
               
               
                 Ex. 
                   
                   
                   
                 meth- 
                 meth- 
                 MS 
                   1 H NMR data 
               
               
                 No. 
                 Compound name 
                 Structure 
                 Starting amine 
                 od 
                 od 
                 ES+ 
                 δ ppm 
               
               
                   
               
             
            
               
                 43 
                 5-cyclobutyl-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Cyclobutanamine (CAS 2516-34-9) 
                 1 
                 D 
                 329 
                 (400 MHz, DMSO-d 6 )  1.65-1.82 (m, 1 H) 1.84-1.95  (m, 1 H) 2.24-2.35 (m, 2 H)  3.05-3.22 (m, 2 H) 4.95-5.06  (m, 1 H) 7.50-7.62 (m, 5 H)  7.93 (d, J = 3 Hz, 1 H)  8.00-8.07 (m, 2 H) 8.13 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                 44 
                 5-(2-methylcyclohexyl)-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-Methylcyclohexyl- amine, mixture of cis  and trans (CAS 7003-32-9) 
                 1 
                 E 
                 371 
                 Mix of diastereoisomers in ~7:3  ratio (400 MHz, DMSO-d 6 )  0.49-0.62 (m, 2.1 H) 0.77-0.84  (m, 0.9 H) 1.26-1.57 (m, 3 H)  1.65-1.75 (m, 2 H) 1.77-1.91  (m, 2 H) 2.31-2.47 (m, 1 H)  2.62-2.71 (m, 1 H) 4.00-4.12  (m, 0.7 H) 4.39-4.50 (m, 0.3 H)  7.52-7.69 (m, 5 H) 7.84-7.90  (m, 1 H) 7.99-8.15 (m, 3 H) 
               
               
                   
               
               
                 45 
                 5-butyl-7-5(phenylsulfonyl)- 5H-pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Butan-1-amine (CAS 109-73-9) 
                 2 
                 D 
                 331 
                 (400 MHz, METHANOL-d 4 )  0.95 (t, J = 7 Hz, 3 H) 1.30-1.42  (m, 2 H) 1.67-1.78 (m, 2 H)  4.17 (t, J = 7 Hz, 2 H) 7.47-7.61  (m, 3 H) 7.92 (d, J = 3 Hz, 1 H)  8.04-8.15 (m, 3 H) 
               
               
                   
               
               
                 46 
                 5-phenethyl-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-phenylethanamine (CAS 64-04-0) 
                 2 
                 E Note 1   
                 379 
                 (400 MHz, METHANOL-d 4 )  3.05 (t, J = 7 Hz, 2 H) 4.42 (t,  J = 7 Hz, 2 H) 6.97-7.11 (m, 5  H) 7.50-7.64 (m, 3 H) 7.83 (d,  J = 3 Hz, 1 H) 8.02 (d, J = 3 Hz,  1 H) 8.07 (d, J = 8 Hz, 2 H) 
               
               
                   
               
               
                 47 
                 2-(6-amino-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-5- yl)cyclohexanol 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-aminocyclohexanol (CAS 6850-38-0) 
                 3 
                 D 
                 373 
                 (400 MHz, DMSO-d 6 )  1.32-1.51 (m, 3 H) 1.61-1.73  (m, 2 H) 1.76-1.88 (m, 2 H)  4.09-4.17 (m, 1 H) 4.86 (d,  J = 13 Hz, 1 H) 7.50-7.64 (m, 3  H) 7.89 (d, J = 3 Hz, 1 H)  8.00-8.09 (m, 2 H) 8.12 (d,  J = 3 Hz, 1 H), further 1 H  multiplet obscured by DMSO 
               
               
                   
               
               
                 48 
                 5-(2-cyclopropylethyl)-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-cyclopropyl- ethanamine (CAS 62893-54-3) 
                 3 
                 D 
                 343 
                 (400 MHz, DMSO-d 6 )  −0.19-−0.12 (m, 2 H) 0.16-0.24  (m, 2 H) 0.55-0.67 (m, 1 H)  1.47-1.58 (m, 2 H) 4.15-4.25  (m, 2 H) 7.49-7.71 (m, 5 H)  7.88 (d, J = 3 Hz, 1 H)  7.99-8.06 (m, 2 H) 8.09 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                 49 
                 5-(4,4-difluoro-cyclohexyl)- 7-(phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4,4-difluoro- cyclohexanamine  hydrochloride (CAS 675112-70-6) 
                 3 
                 C 
                 393 
                 (400 MHz,  DICHLOROMETHANE-d 2 )  1.88-2.11 (m, 4 H) 2.25-2.39  (m, 2 H) 2.76-2.94 (m, 2 H)  4.29-4.45 (m, 1 H) 6.21 (br. s.,  2 H) 7.47-7.63 (m, 3 H) 7.97 (d,  J = 3 Hz, 1 H) 8.18 (d, J = 7 Hz,  2 H) 8.24 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                 50 
                 5-(2-cyclobutylethyl)-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-cyclobutyl- ethanamine (CAS 60637-97-0) 
                 3 Note 2   
                 E 
                 357 
                 (400 MHz, DMSO-d 6 )  1.46-1.58 (m, 2 H) 1.65-1.79  (m, 4 H) 1.80-1.91 (m, 2 H)  2.13-2.24 (m, 1 H) 4.05 (t, J = 7  Hz, 2 H) 7.51-7.71 (m, 5 H)  7.90 (d, J = 3 Hz, 1 H) 8.03 (d,  J = 7 Hz, 2 H) 8.11 (d, J = 3 Hz,  1 H) 
               
               
                   
               
               
                 51 
                 7-(phenylsulfonyl)-5- (tetrahydro-2H-pyran-3-yl)- 5H-pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 tetrahydro-2H-pyran- 3-amine  hydrochloride (CAS 675112-58-0) 
                 3 Note 2   
                 E 
                 359 
                 (400 MHz, DMSO-d 6 )  1.69-1.80 (m, 2 H) 1.84-1.94  (m, 1 H) 2.56-2.70 (m, 1 H)  3.36-3.45 (m, 1 H) 3.79-3.91  (m, 2 H) 4.26 (t, J = 10 Hz, 1  H) 4.52-4.62 (m, 1 H) 7.51-7.63  (m, 3 H) 7.71 (br. s., 2 H) 7.90  (d, J = 3 Hz, 1 H) 8.05 (d, J = 7  Hz, 2 H) 8.11 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                 52 
                 5-(3,3-dimethylbutyl)-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3,3-dimethylbutan-1- amine (CAS 15673-00-4) 
                 3 
                 E 
                 359 
                 (400 MHz, DMSO-d 6 ) 0.95 (s,  9 H) 1.45-1.56 (m, 2 H)  4.09-4.19 (m, 2 H) 7.50-7.66  (m, 5 H) 7.92 (d, J = 3 Hz, 1 H)  8.01-8.07 (m, 2 H) 8.11 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                 53 
                 5-((1R*,2R*,4S*)- bicyclo[2.2.1]heptan-2-yl)-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b)pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 exo-2- aminonorbornane (CAS 7242-92-4,  Sigma-Aldrich cat.  no. 179604) 
                 3 
                 E 
                 369 
                 (400 MHz, DMSO-d 6 )  1.14-1.29 (m, 2 H) 1.43-1.59  (m, 3 H) 1.80-1.91 (m, 1 H)  2.29-2.42 (m, 2 H) 2.43-2.48  (m, 1 H) 2.60-2.67 (m, 1 H)  4.22-4.31 (m, 1 H) 7.45-7.64  (m, 5 H) 7.86 (d, J = 3 Hz, 1 H)  8.00-8.11 (m, 3 H) 
               
               
                   
               
               
                 54 
                 5-(cyclopentylmethyl)-7- (phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Cyclopentyl- methanamine (CAS 6053-81-2) 
                 3 
                 E 
                 357 
                 (400 MHz, DMSO-d 6 )  1.17-1.32 (m, 2 H) 1.39-1.69  (m, 6 H) 2.35-2.45 (m, 1 H)  4.08 (d, J = 8 Hz, 2 H)  7.51-7.61 (m, 3 H) 7.65 (br. s, 2  H) 7.89 (d, J = 3 Hz, 1 H)  8.01-8.07 (m, 2 H) 8.10 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                 55 
                 5-((1-ethylcyclopropyl)- methyl)-7-(phenylsulfonyl)- 5H-pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (1-ethylcyclopropyl)  methanamine (CAS 1177326-74-7) 
                 3 
                 E 
                 357 
                 (400 MHz, DMSO-d 6 )  0.27-0.34 (m, 2 H) 0.51-0.58  (m, 2 H) 0.79 (t, J = 7 Hz, 3 H)  1.26 (q, J = 7 Hz, 2 H) 4.17 (s,  2 H) 7.46-7.62 (m, 5 H) 7.91 (d,  J = 3 Hz, 1 H) 7.99-8.07 (m, 2  H) 8.11 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                 56 
                 5-((2,2- dimethylcyclopropyl)methyl)- 7-(phenylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (2,2- dimethylcyclopropyl)  methanamine (CAS 725743-45-3) 
                 3 
                 E 
                 357 
                 (400 MHz, DMSO-d 6 )  0.30-0.40 (m, 2 H) 1.00 (s, 3 H)  1.02-1.11 (m, 1 H) 1.19 (s, 3 H)  3.94-4.05 (m, 1 H) 4.28-4.38  (m, 1 H) 7.51-7.70 (m, 5 H)  7.90 (d, J = 3.03 Hz, 1 H)  8.02-8.08 (m, 2 H) 8.11 (d,  J = 3.03 Hz, 1 H) 
               
               
                   
               
               
                   Note 1 Followed by flash chromatography (12 g silica, 25-60% EtOAc/petrol) 
               
               
                   Note 2 Aqueous workup 
               
            
           
         
       
     
     Examples 57 to 107 (see Table 3 following) were prepared according to one of the procedures 4 or 5 as described below. 
     Procedure 4 
     To a solution of 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl chloride (Intermediate 41; 50 mg, 0.112 mmol) in THF (1 mL) was added triethylamine (0.089 mL, 0.635 mmol) and a primary or secondary amine (0.175 mmol). The reaction was stirred at rt for 3 hours and then ethanol (1 mL) and hydrazine monohydrate (0.635 mmol) were added. The reaction mixture was warmed to 80° C. and maintained at this temperature overnight. The reaction mixtures were filtered and concentrated. The residue was taken up in DCM and washed with water, then the organic phase was separated and concentrated and the resulting crude product was purified via prep HPLC using one of the methods listed below or column chromatography on silica. 
     Procedure 5 
     To a solution of 5-cyclohexyl-6-(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)-5H-pyrrolo[2,3-b]pyrazine-7-sulfonyl chloride (Intermediate 41; 55 mg, 0.124 mmol) in THF (1 mL) was added triethylamine (0.052 mL, 0.371 mmol) and a primary or secondary amine (00.247 mmol). After 2 h at room temperature the mixture was diluted with water and extracted with DCM. The organic phase was concentrated, then ethanol (1 mL) and hydrazine monohydrate (0.018 mL, 0.371 mmol) were added and the reaction mixture was warmed to 70° C. for 3 h. The reaction mixture was filtered and concentrated and the residue was purified by column chromatography (silica, 0-100% EtOAc/petroleum ether). 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 HPLC 
                 Gradient (acetonitrile/water 
               
               
                   
                 Method 
                 (with 0.1% ammonia)) 
               
               
                   
                   
               
             
            
               
                   
                 A 
                  5-25% 
               
               
                   
                 B 
                  5-40% 
               
               
                   
                 C 
                 10-50% 
               
               
                   
                 D 
                 20-60% 
               
               
                   
                 E 
                 30-70% 
               
               
                   
                 F 
                 40-80% 
               
               
                   
                 G 
                 55-95% 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
               
                   
                   
                   
                   
                 Syn- 
                   
                   
                   
               
               
                   
                   
                   
                   
                 thesis 
                   
                   
                   
               
               
                 Ex. 
                   
                   
                   
                 meth- 
                 Purification 
                 MS 
                   1 H NMR data 
               
               
                 No. 
                 Name of compound 
                 Structure 
                 Starting amine 
                 od 
                 method 
                 ES+ 
                 δ ppm 
               
               
                   
               
             
            
               
                  57 
                 5-cyclohexyl-7- (piperidin-1- ylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Piperidine (CAS 110-89- 4) 
                 2 
                 0-100% ethyl  acetate/petrol 
                 364 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.32-1.57 (m, 5 H)  1.59-1.71 (m, 4 H)  1.74-1.84 (m, 1 H)  1.87-2.07 (m, 4 H)  2.38-2.55 (m, 2 H)  3.08-3.29 (m, 4 H)  4.19-4.39 (m, 1 H) 5.97  (br. s., 2 H) 7.89-7.98 (m,  1 H) 8.14-8.23 (m, 1 H) 
               
               
                   
               
               
                  58 
                 5-cyclohexyl-7- (pyrrolidin-1- ylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Pyrrolidine (CAS 123-75- 1) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 350 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.29-1.57 (m, 4 H)  1.73-1.84 (m, 4 H)  1.87-2.04 (m, 4 H)  2.37-2.56 (m, 2 H)  3.39-3.52 (m, 4 H)  4.23-4.38 (m, 1 H) 6.01  (br. s., 2 H) 7.94 (d, J = 3  Hz, 1 H) 8.18 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  59 
                 6-amino-5-cyclohexyl- N-propyl-5H- pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Propan-1- amine (CAS 107-10- 8) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 338 
                 (400 MHz, DMSO-d 6 )  0.74 (t, J = 7 Hz, 3 H)  1.18-1.47 (m, 5 H)  1.66-1.76 (m, 3 H)  1.80-1.89 (m, 2 H)  2.42-2.57 (m, 2 H)  2.70-2.79 (m, 2 H)  4.31-4.44 (m, 1 H) 7.14  (t, J = 6 Hz, 1 H) 7.21 (s,  2 H) 7.88 (d, J = 3 Hz, 1  H) 8.08 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                  60 
                 6-amino-5-cyclohexyl- N-methyl-N-propyl- 5H-pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N- methylpropan- 1-amine (CAS 627-35- 0) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 352 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 0.94 (t, J = 7 Hz, 3 H)  1.33-1.65 (m, 5 H)  1.76-1.84 (m, 1 H)  1.88-2.06 (m, 4 H)  2.38-2.51 (m, 2 H) 2.84  (s, 3 H) 3.09-3.17 (m, 2  H) 4.23-4.36 (m, 1 H)  6.00 (br. s., 2 H) 7.95 (d,  J = 3 Hz, 1 H) 8.18 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                  61 
                 5-cyclohexyl-7- (morpholinosulfonyl)- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Morpholine (CAS 110-91- 8) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 366 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.33-1.59 (m, 3 H)  1.77-1.85 (m, 1 H)  1.88-2.06 (m, 4 H)  2.38-2.57 (m, 2 H)  3.14-3.27 (m, 4 H)  3.70-3.81 (m, 4 H)  4.22-4.40 (m, 1 H) 6.02  (br. s., 2 H) 7.97 (d, J = 3  Hz, 1 H) 8.19 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  62 
                 5-cyclohexyl-7-((4- methylpiperidin-1- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-methyl- piperidine (CAS 626-58- 4) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 378 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 0.81-0.97 (m, 3 H)  1.24-1.57 (m, 6 H)  1.64-1.73 (m, 2 H)  1.76-1.84 (m, 1 H)  1.89-2.05 (m, 4 H)  2.38-2.50 (m, 2 H)  2.52-2.62 (m, 2 H)  3.76-3.85 (m, 2 H)  4.20-4.39 (m, 1 H) 6.00  (br. s., 2 H) 7.95 (d, J = 3  Hz, 1 H) 8.18 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  63 
                 5-cyclohexyl-7-((4- methylpiperazin-1- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 1-methyl- piperazine (CAS 109-01- 3) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 379 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.35-1.57 (m, 3 H)  1.76-1.85 (m, 1 H)  1.89-2.03 (m, 4 H) 2.25  (s, 3 H) 2.37-2.57 (m, 6  H) 3.17-3.30 (m, 4 H)  4.22-4.34 (m, 1 H) 5.92  (br. s., 2H) 7.95 (d, J = 3  Hz, 1 H) 8.18 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  64 
                 5-cyclohexyl-7-((3- methoxyazetidin-1- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-metboxy- azetidine (CAS 110925- 17-2) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 366 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.33-1.58 (m, 3 H)  1.76-1.84 (m, 1 H)  1.90-2.04 (m, 4 H)  2.40-2.55 (m, 2 H) 3.14  (s, 3 H) 3.82-3.90 (m, 2  H) 4.00-4.06 (m, 1 H)  4.07-4.14 (m, 2 H)  4.25-4.38 (m, 1 H) 6.09  (br. s., 2 H) 7.99 (d, J = 3  Hz, 1 H) 8.22 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  65 
                 5-cyclohexyl-7-((4- ethoxypiperidin-1- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-ethoxy- piperidine (CAS 1122-86- 7) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 408 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.05-1.15 (m, 3 H)  1.26-1.51 (m, 3 H)  1.57-1.66 (m, 2 H)  1.75-1.83 (m, 1 H)  1.84-1.98 (m, 6 H)  2.38-2.53 (m, 2 H)  2.96-3.05 (m, 2 H)  3.28-3.37 (m, 1 H)  3.37-3.48 (m, 4 H) 4.26  (br. s., 1 H) 6.01 (br. s., 2  H) 7.92 (d, J = 3 Hz, 1 H)  8.14 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                  66 
                 5-cyclohexyl-7-((4,4- dimethylpiperidin-1- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4,4-dimethyl- piperidine  hydrochloride (CAS 38646- 68-3) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 392 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 0.88 (s, 6 H)  1.32-1.56 (m, 7 H)  1.76-1.84 (m, 1 H)  1.89-2.03 (m, 4 H)  2.39-2.53 (m, 2 H)  3.16-3.26 (m, 4 H)  4.23-4.36 (m, 1 H) 5.99  (br. s., 2 H) 7.95 (d, J = 3  Hz, 1 H) 8.19 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  67 
                 5-cyclohexyl-7-((3- methylpyrrolidin-1- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-methyl- pyrrolidine (CAS 34375- 89-8) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 364 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 0.95 (d, J = 7 Hz, 3  H) 1.34-1.57 (m, 4 H)  1.77-1.85 (m, 1 H)  1.88-2.04 (m, 5 H)  2.08-2.22 (m, 1 H)  2.39-2.54 (m, 2 H)  2.90-2.98 (m, 1 H)  3.39-3.49 (m, 1 H)  3.59-3.73 (m, 2 H)  4.24-4.39 (m, 1 H) 6.05  (br. s., 2 H) 7.95 (d, J = 3  Hz, 1 H) 8.18 (d, J = 3  Hz, 1H) 
               
               
                   
               
               
                  68 
                 5-cyclohexyl-7-((2- methylpyrrolidin-1- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-methyl- pyrrolidine (CAS) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 364 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.32 (d, J = 7 Hz, 3  H) 1.35-1.63 (m, 5 H)  1.69-1.87 (m, 3 H)  1.89-2.04 (m, 4 H)  2.37-2.51 (m, 2 H)  3.39-3.55 (m, 2 H)  4.18-4.36 (m, 2 H) 6.03  (br. s., 2 H) 7.94 (d, J = 3  Hz, 1 H) 8.18 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  69 
                 5-cyclohexyl-7-((4,4- difluoropiperidin-1- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4,4-difluoro- piperidine (CAS 21987- 29-1) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 400 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.34-1.56 (m, 3 H)  1.77-1.84 (m, 1 H)  1.89-2.02 (m, 4 H)  2.04-2.17 (m, 4 H)  2.39-2.54 (m, 2 H)  3.37-3.48 (m, 4 H)  4.21-4.38 (m, 1 H) 6.02  (br. s., 2 H) 7.93-8.01 (m,  1 H) 8.15-8.23 (m, 1 H) 
               
               
                   
               
               
                  70 
                 6-amino-N-benzyl-5- cyclohexyl-5H- pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Benzylamine (CAS 100-46- 9) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 386 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.33-1.55 (m, 3 H)  1.75-1.85 (m, 1 H)  1.86-2.06 (m, 4 H)  2.33-2.51 (m, 2 H) 4.10  (d, J = 7 Hz, 2 H)  4.18-4.28 (m, 1 H) 5.51  (t, J = 6 Hz, 1 H) 5.87  (br. s., 2 H) 7.12-7.22 (m,  5 H) 7.95 (d, J = 3 Hz, 1  H) 8.15 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                  71 
                 6-amino-N,5- dicyclohexyl-N- methyl-5H- pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-methyl- cyclohexan- amine (CAS 100-60- 7) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 392 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.00-1.11 (m, 1 H)  1.27-1.65 (m, 10 H)  1.68-1.83 (m, 3 H)  1.86-2.05 (m, 4 H)  2.38-2.54 (m, 2 H) 2.83  (s, 3 H) 3.83-3.94 (m, 1  H) 4.21-4.35 (m, 1 H)  5.97 (br. s., 2 H) 7.93 (d,  J = 3 Hz, 1 H) 8.19 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                  72 
                 5-cyclohexyl-7-(1,4- oxazepane-4-sulfonyl)- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 1,4-oxazepane  hydrochloride (CAS 178312- 62-4) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 380 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.33-1.55 (m, 3 H)  1.76-1.86 (m, 1 H)  1.89-2.04 (m, 6 H)  2.37-2.53 (m, 2 H)  3.51-3.61 (m, 4 H)  3.71-3.80 (m, 4 H)  4.20-4.36 (m, 1 H) 5.94  (br. s., 2 H) 7.95 (d, J = 3  Hz, 1 H) 8.18 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  73 
                 5-cyclohexyl-7-(4- methoxypiperidine-1- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-methoxy- piperidine (CAS 4045-24- 3) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 394 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.35-1.55 (m, 3 H)  1.64-1.73 (m, 2 H)  1.77-1.84 (m, 1 H)  1.87-2.04 (m, 6 H)  2.39-2.54 (m, 2 H)  3.05-3.13 (m, 2 H)  3.18-3.30 (m, 4 H)  3.36-3.45 (m, 2 H)  4.20-4.38 (m, 1 H) 5.98  (br. s., 2 H) 7.96 (d, J = 3  Hz, 1 H) 8.19 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  74 
                 6-amino-N- (cyclobutylmethyl)-5- cyclohexyl-5H- pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Cyclobutyl- methanamine  hydrochloride (CAS 5454-82- 0) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 364 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.35-1.55 (m, 3 H)  1.57-1.68 (m, 2 H)  1.75-2.05 (m, 9 H)  2.39-2.54 (m, 3 H)  2.82-2.91 (m, 2 H)  4.21-4.34 (m, 1 H) 5.04  (t, J = 6 Hz, 1 H) 5.88  (br. s., 2 H) 7.98 (d, J = 3  Hz, 1 H) 8.18 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  75 
                 5-cyclohexyl-7-(3,3- dimethylpyrrrolidine- 1-sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3,3-Dimethyl- pyrrolidine (CAS 3437-30- 7) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 378 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 0.97 (s, 6 H)  1.33-1.53 (m, 3 H) 1.58  (t, J = 7 Hz, 2 H)  1.77-1.85 (m, 1 H)  1.90-2.04 (m, 4 H)  2.38-2.53 (m, 2 H) 3.18  (s, 2 H) 3.61 (t, J = 7 Hz,  2 H) 4.24-4.35 (m, 1 H)  5.96 (br. s., 2 H) 7.95 (d,  J = 3 Hz, 1 H) 8.19 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                  76 
                 5-cyclohexyl-7-(2,6- dimethylmorpholine-4- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2,6-dimethyl- morpholine  (mixture of  stereoisomers;  CAS 141-91-3) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 394 
                 Mixture of  diasteroisomers in ~4:1  ratio. (400 MHz,  DICHLOROMETHANE- d 2 ) 1.11-1.16 (m, 4.5 H)  1.21-1.27 (m, 1.5 H)  1.35-1.58 (m, 3 H)  1.77-1.85 (m, 1 H)  1.89-2.07 (m, 4 H)  2.28-2.39 (m, 1.6 H)  2.41-2.53 (m, 2 H)  2.85-2.95 (m, 0.4 H)  3.20-3.28 (m, 0.4 H)  3.59-3.65 (m, 1.6 H)  3.67-3.75 (m, 1.6 H)  4.01-4.11 (m, 0.4 H)  4.23-4.36 (m, 1 H) 5.96  (br. s., 2 H) 7.92-8.01 (m,  1 H) 8.16-8.23 (m, 1 H) 
               
               
                   
               
               
                  77 
                 7-(azepane-1- sulfonyl)-5- cyclohexyl-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Azepane (CAS 111-49- 9) 
                 1 
                 0-100% ethyl  acetate/petrol 
                 378 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.33-1.57 (m, 3 H)  1.58-1.64 (m, 4 H)  1.69-1.84 (m, 5 H)  1.88-2.04 (m, 4 H)  2.36-2.51 (m, 2 H)  3.37-3.46 (m, 4 H)  4.22-4.34 (m, 1 H) 5.92  (br. s., 2 H) 7.94 (d, J = 3  Hz, 1 H) 8.18 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  78 
                 5-cyclohexyl-7- (thiomorpholine-4- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Thio- morpholine (CAS 123-90- 0) 
                 1 
                 E 
                 382 
                 (400 MHz, DMSO-d 6 )  1.21-1.33 (m, 1 H)  1.35-1.50 (m, 2 H)  1.64-1.78 (m, 3 H)  1.81-1.90 (m, 2 H)  2.43-2.56 (m, 2 H)  2.61-2.70 (m, 4 H)  3.32-3.40 (m, 4 H)  4.35-4.47 (m, 1 H) 7.32  (s, 2 H) 7.91 (d, J = 3 Hz,  1 H) 8.08 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                  79 
                 N-(1-{6-amino-5- cyclohexyl-5H- pyrrolo[2,3- b]pyrazine-7- sulfonyl}piperidin-4- yl)-N-methylacetamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 N-methyl-N- (piperidin-4- yl)acetamide (CAS 83180- 55-6) 
                 1 
                 D 
                 435 
                 (400 MHz, DMSO-d 6 )  1.24-1.33 (m, 1 H)  1.36-1.49 (m, 3 H)  1.56-1.79 (m, 6 H)  1.81-1.88 (m, 2 H)  1.90-1.97 (m, 3 H)  2.52-2.61 (m, 4 H)  2.63-2.72 (m, 2 H)  3.53-3.68 (m, 1 H)  3.75-3.85 (m, 2 H)  4.10-4.22 (m, 1 H)  4.36-4.47 (m, 1 H)  7.25-7.37 (m, 2 H)  7.86-7.94 (m, 1 H)  8.06-8.13 (m, 1 H) 
               
               
                   
               
               
                  80 
                 6-amino-5-cyclohexyl- N-(oxetan-3-ylmethyl)- 5H-pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Oxetan-3-yl- methanamine (CAS 6246-05- 5) 
                 1 
                 D 
                 366 
                 (400 MHz, DMSO-d 6 )  1.23-1.33 (m, 1 H)  1.35-1.50 (m, 2 H)  1.66-1.78 (m, 3 H)  1.82-1.90 (m, 2 H)  2.43-2.57 (m, 2 H)  2.89-3.03 (m, 1 H)  3.05-3.12 (m, 2 H)  4.12-4.19 (m, 2 H)  4.34-4.43 (m, 1 H)  4.45-4.51 (m, 2 H) 7.23  (s, 2 H) 7.38 (t, J = 6 Hz,  1 H) 7.90 (d, J = 3 Hz, 1  H) 8.09 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                  81 
                 7-(4-benzylpiperidine- 1-sulfonyl)-5- cyclohexyl-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-benzyl- piperidine (CAS 31252- 42-3) 
                 1 
                 F 
                 454 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.29-1.56 (m, 5 H)  1.67-1.73 (m, 2 H)  1.77-1.84 (m, 1 H)  1.87-1.94 (m, 2 H)  1.95-2.05 (m, 3 H)  2.37-2.59 (m, 6 H)  3.76-3.86 (m, 2 H)  4.22-4.34 (m, 1 H) 5.91  (s, 2 H) 7.08-7.15 (m, 2  H) 7.15-7.22 (m, 1 H)  7.24-7.31 (m, 2 H) 7.94  (d, J = 3 Hz, 1 H) 8.17 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                  82 
                 6-amino-5-cyclohexyl- N-(3,3,3- trifluoropropyl)-5H- pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3,3,3-trifluoro- propan-1- amine (CAS 460-39- 9) 
                 1 
                 E 
                 392 
                 (400 MHz, DMSO-d 6 )  1.23-1.32 (m, 1 H)  1.34-1.49 (m, 2 H)  1.65-1.78 (m, 3 H)  1.81-1.90 (m, 2 H)  2.34-2.55 (m, 4 H)  3.05-3.13 (m, 2 H)  4.33-4.45 (m, 1 H) 7.26  (s, 2 H) 7.36-7.44 (m, 1  H) 7.90 (d, J = 3 Hz, 1 H)  8.08 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                  83 
                 5-cyclohexyl-7-(4- phenylpiperidine-1- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-phenyl- piperidine (CAS 771-99- 3) 
                 1 
                 F 
                 440 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.34-1.58 (m, 3 H)  1.77-2.07 (m, 9 H)  2.39-2.56 (m, 3 H)  2.68-2.78 (m, 2 H)  3.92-4.07 (m, 2 H)  4.25-4.37 (m, 1 H) 5.96  (s, 2 H) 7.15-7.25 (m, 3  H) 7.28-7.34 (m, 2 H)  7.98 (d, J = 3 Hz, 1 H)  8.22 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                  84 
                 6-amino-5-cyclohexyl- N-(2-phenylethyl)-5H- pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-phenyl- ethanamine (CAS 64-04-0) 
                 1 
                 F 
                 400 
                 (400 MHz, DMSO-d 6 )  1.21-1.32 (m, 1 H)  1.35-1.49 (m, 2 H)  1.64-1.77 (m, 3 H)  1.80-1.90 (m, 2 H)  2.43-2.55 (m, 2 H)  2.61-2.69 (m, 2 H)  3.01-3.09 (m, 2 H)  4.32-4.44 (m, 1 H)  7.04-7.16 (m, 3 H)  7.17-7.26 (m, 5 H) 7.88  (d, J = 3 Hz, 1 H) 8.06 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                  85 
                 5-cyclohexyl-7-(4- phenoxypiperidine-1- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-phenoxy- piperidine (CAS 3202-33- 3) 
                 1 
                 F 
                 456 
                 (400 MHz, DMSO-d 6 )  1.25-1.33 (m, 1 H)  1.36-1.50 (m, 2 H)  1.56-1.79 (m, 5 H)  1.83-1.90 (m, 2 H)  1.94-2.04 (m, 2 H)  2.46-2.58 (m, 2 H)  2.85-2.96 (m, 2 H)  3.44-3.53 (m, 2 H)  4.35-4.48 (m, 2 H)  6.82-6.91 (m, 3 H)  7.17-7.24 (m, 2 H) 7.31  (s, 2 H) 7.91 (d, J = 3 Hz,  1 H) 8.10 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                  86 
                 5-cyclohexyl-7-(3- phenylpyrrolidine-1- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-phenyl- pyrrolidine (CAS 936-44- 7) 
                 1 
                 F 
                 426 
                 (400 MHz, DMSO-d 6 )  1.22-1.35 (m, 1 H)  1.35-1.51 (m, 2 H)  1.66-1.79 (m, 4 H)  1.82-1.93 (m, 2 H)  2.03-2.13 (m, 1 H)  2.46-2.57 (m, 2 H)  3.13-3.28 (m, 2 H)  3.33-3.43 (m, 1 H)  3.61-3.70 (m, 1 H)  3.89-3.98 (m, 1 H)  4.36-4.51 (m, 1 H)  7.04-7.13 (m, 2 H)  7.14-7.28 (m, 3 H) 7.31  (s, 2 H) 7.92 (d, J = 3 Hz,  1 H) 8.11 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                  87 
                 5-cyclohexyl-7-[4- (trifluoromethyl)  piperidine-1-sulfonyl]- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-(trifluoro- methyl)- piperidine (CAS 657-36- 3) 
                 1 
                 F 
                 432 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.34-1.54 (m, 3 H)  1.66-1.75 (m, 2 H)  1.77-1.86 (m, 1 H)  1.89-2.11 (m, 7 H)  2.39-2.52 (m, 2 H)  2.60-2.70 (m, 2 H)  3.93-4.03 (m, 2 H)  4.24-4.35 (m, 1 H) 5.94  (br. s., 2 H) 7.97 (d, J = 3  Hz, 1 H) 8.19 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                  88 
                 5-cyclohexyl-7-[3- (methoxyrnethyl)  pyrrolidine-1- sulfonyl]-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-(methoxy- methyl)- pyrrolidine (CAS 936940- 38-4) 
                 1 
                 E 
                 394 
                 (400 MHz, DMSO-d 6 )  1.24-1.33 (m, 1 H)  1.36-1.50 (m, 3 H)  1.65-1.80 (m, 4 H)  1.81-1.90 (m, 2 H)  2.20-2.30 (m, 1 H)  2.43-2.56 (m, 2 H)  2.95-3.13 (m, 6 H)  3.23-3.29 (m, 1 H)  3.38-3.50 (m, 2 H)  4.34-4.45 (m, 1 H) 7.29  (s, 2 H) 7.90 (d, J = 3 Hz,  1 H) 8.08 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                  89 
                 6-amino-5-cyclohexyl- N- (cyclopropylmethyl)- 5H-pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Cyclopropyl- methanamine (CAS 2516-47- 4) 
                 1 
                 E 
                 350 
                 (400 MHz, DMSO-d 6 )  −0.03-0.05 (m, 2 H)  0.20-0.29 (m, 2 H)  0.72-0.80 (m, 1 H)  1.22-1.31 (m, 1 H)  1.34-1.47 (m, 2 H)  1.62-1.74 (m, 3 H)  1.78-1.90 (m, 2 H)  2.40-2.52 (m, 2 H)  2.67-2.73 (m, 2 H)  4.31-4.43 (m, 1 H)  7.11-7.24 (m, 3 H) 7.86  (d, J = 3 Hz, 1 H) 8.06 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                  90 
                 6-amino-5-cyclohexyl- N-(2-methoxyethyl)- 5H-pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-methoxy- ethanamine (CAS 109-85- 3) 
                 1 
                 D 
                 354 
                 (400 MHz, DMSO-d 6 )  1.24-1.34 (m, 1 H)  1.36-1.49 (m, 2 H)  1.66-1.78 (m, 3 H)  1.81-1.90 (m, 2 H)  2.44-2.56 (m, 2 H)  2.95-3.03 (m, 2 H) 3.11  (s, 3 H) 3.25-3.30 (m, 2  H) 4.34-4.44 (m, 1 H)  7.06-7.13 (m, 1 H) 7.21  (s, 2 H) 7.89 (d, J = 3 Hz,  1 H) 8.08 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                  91 
                 5-cyclohexyl-7-(3- methoxypyrrolidine-1- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-methoxy- pyrrolidine (CAS 62848- 20-8) 
                 1 
                 E 
                 380 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.34-1.44 (m, 1 H)  1.46-1.60 (m, 2 H)  1.78-1.84 (m, 1 H)  1.86-2.03 (m, 6 H)  2.39-2.55 (m, 2 H) 3.14  (s, 3 H) 3.45-3.63 (m, 4  H) 3.83-3.91 (m, 1 H)  4.31-4.44 (m, 1 H)  6.22-6.45 (m, 2 H) 7.97  (d, J = 3 Hz, 1 H) 8.18 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                  92 
                 5-cyclohexyl-7-(3,3- dimethylpiperidine-1- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3,3-dimethyl- piperidine (CAS 1193-12- 0) 
                 1 
                 F 
                 392 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 0.99 (s, 6 H)  1.22-1.28 (m, 2 H)  1.35-1.55 (m, 3 H)  1.66-1.73 (m, 2 H)  1.77-1.86 (m, 1 H)  1.89-2.04 (m, 4 H)  2.37-2.54 (m, 2 H) 2.82  (s, 2 H) 3.09-3.19 (m, 2  H) 4.19-4.35 (m, 1 H)  5.91 (br. s., 2 H) 7.95 (d,  J = 3 Hz, 1 H) 8.19 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                  93 
                 1-{6-amino-5- cyclohexyl-5H- pyrrolo[2,3- b]pyrazine-7- sulfonyl}piperidin-4-ol 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 piperidin-4-ol (CAS 5382-16- 1) 
                 1 
                 D 
                 380 
                 (400 MHz, DMSO-d 6 )  1.22-1.33 (m, 1 H)  1.35-1.48 (m, 4 H)  1.68-1.78 (m, 5 H)  1.80-1.91 (m, 2 H)  2.43-2.56 (m, 2 H)  2.77-2.87 (m, 2 H)  3.27-3.37 (m, 2 H)  3.47-3.56 (m, 1 H)  4.34-4.45 (m, 1 H) 4.59  (d, J = 4 Hz, 1 H) 7.27 (s,  2 H) 7.89 (d, J = 3 Hz, 1  H) 8.08 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                  94 
                 5-cyclohexyl-7- (1,2,3,4- tetrahydroisoquinoline- 2-sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 1,2,3,4- tetrahydro- isoquinoline (CAS 91-21-4) 
                 1 
                 F 
                 412 
                 (400 MHz, DMSO-d 6 )  1.19-1.32 (m, 1 H)  1.35-1.48 (m, 2 H)  1.65-1.77 (m, 3 H)  1.79-1.90 (m, 2 H)  2.39-2.48 (m, 2 H)  2.80-2.88 (m, 2 H)  3.40-3.47 (m, 2 H)  4.31-4.43 (m, 3 H)  6.99-7.14 (m, 4 H) 7.35  (s, 2 H) 7.85 (d, J = 3 Hz,  1 H) 8.05 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                  95 
                 6-amino-N-(butan-2- yl)-5-cyclohexyl-5H- pyrrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 butan-2-amine (CAS 13952- 84-6) 
                 1 
                 E 
                 352 
                 (400 MHz, DMSO-d 6 )  0.64 (t, J = 7 Hz, 3 H)  0.88 (d, J = 7 Hz, 3 H)  1.21-1.33 (m, 3 H)  1.36-1.49 (m, 2 H)  1.65-1.77 (m, 3 H)  1.80-1.92 (m, 2 H)  2.44-2.57 (m, 2 H)  3.08-3.19 (m, 1 H)  4.31-4.44 (m, 1 H)  7.02-7.11 (m, 1 H) 7.19  (s, 2 H) 7.88 (d, J = 3 Hz,  1 H) 8.08 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                  96 
                 6-amino-5-cyclohexyl- N-(oxolan-2- ylmethyl)-5H- pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (tetrahydro- furan-2-yl)- methanamine (CAS 4795-29- 3) 
                 1 
                 E 
                 380 
                 (400 MHz, DMSO-d 6 )  1.23-1.33 (m, 1 H)  1.36-1.55 (m, 3 H)  1.64-1.91 (m, 8 H)  2.42-2.54 (m, 2 H)  2.77-2.94 (m, 2 H)  3.46-3.56 (m, 1 H)  3.57-3.67 (m, 1 H)  3.73-3.86 (m, 1 H)  4.31-4.46 (m, 1 H)  7.07-7.15 (m, 1 H) 7.20  (s, 2 H) 7.89 (d, J = 3 Hz,  1 H) 8.08 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                  97 
                 5-cyclohexyl-7-(2,3- dihydro-1H-isoindole- 2-sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 Isoindoline (CAS 496-12- 8) 
                 1 
                 F 
                 398 
                 (400 MHz, DMSO-d 6 )  1.19-1.31 (m, 1 H)  1.34-1.47 (m, 2 H)  1.61-1.78 (m, 3 H)  1.78-1.89 (m, 2 H)  2.38-2.48 (m, 2 H)  4.30-4.44 (m, 1 H) 4.73  (s, 4 H) 7.13-7.26 (m, 4  H) 7.38 (s, 2 H)  7.82-7.85 (m, 1 H)  7.99-8.06 (m, 1 H) 
               
               
                   
               
               
                  98 
                 5-cyclohexyl-7-{4-[(4- fluorophenyl)carbonyl]  piperazine-1-sulfonyl}- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 (4- fluorophenyl)  (piperazin-1- yl)methanone (CAS 102391- 98-0) 
                 1 
                 E 
                 487 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.35-1.57 (m, 3 H)  1.78-1.87 (m, 1 H)  1.91-2.06 (m, 4 H)  2.39-2.53 (m, 2 H)  3.21-3.34 (m, 4 H)  3.55-3.85 (m, 4 H)  4.23-4.35 (m, 1 H) 5.95  (br. s., 2 H) 7.08-7.18 (m,  2 H) 7.33-740 (m, 2 H)  7.98 (d, J = 3 Hz, 1 H)  8.19 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                  99 
                 5-cyclohexyl-7-(3- phenoxyazetidine-1- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-phenoxy- azetidine (CAS 76263- 18-8 
                 1 
                 F 
                 428 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.35-1.56 (m, 3 H)  1.77-1.85 (m, 1 H)  1.92-2.06 (m, 4 H)  2.41-2.56 (m, 2 H)  4.06-4.16 (m, 2 H)  4.25-4.34 (m, 1 H)  4.36-4.41 (m, 2 H)  4.75-4.83 (m, 1 H) 6.00  (br. s., 2 H) 6.55-6.60 (m,  2 H) 6.93-7.01 (m, 1 H)  7.21-7.29 (m, 2 H) 7.99  (d, J = 3 Hz, 1 H) 8.18 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                 100 
                 5-cyclohexyl-7-[3- (piperidin-1- yl)azetidine-1- sulfonyl]-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 1-(azetidin-3- yl)piperidine (CAS 138022- 86-3) 
                 1 
                 E 
                 419 
                 (400 MHz, DMSO-d 6 )  1.13-1.34 (m, 7 H)  1.37-1.52 (m, 2 H)  1.65-1.80 (m, 3 H)  1.83-1.97 (m, 6 H)  2.54-2.60 (m, 2 H)  2.84-2.95 (m, 1 H)  3.69-3.76 (m, 2 H)  3.76-3.83 (m, 2 H)  4.37-4.50 (m, 1 H) 7.32  (s, 2 H) 7.93 (d, J = 3 Hz,  1 H) 8.11 (d, J = 3 Hz, 1  H) 
               
               
                   
               
               
                 101 
                 5-cyclohexyl-7-[3-(1H- pyrazol-1-yl)azetidine- 1-sulfonyl]-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 1-(azetidin-3- yl)-1H- pyrazole (CAS  1107627-16-6) 
                 1 
                 E 
                 402 
                 (400 MHz, DMSO-d 6 )  1.24-1.35 (m, 1 H)  1.37-1.52 (m, 2 H)  1.65-1.94 (m, 5 H)  2.53-2.62 (m, 2 H) 4.22  (d, J = 7 Hz, 4 H)  4.38-4.51 (m, 1 H)  4.97-5.09 (m, 1 H)  6.06-6.14 (m, 1 H)  7.11-7.19 (m, 1 H) 7.35  (s, 2 H) 7.46-7.56 (m, 1  H) 7.91 (d, J = 3 Hz, 1 H)  8.05 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                 102 
                 5-cyclohexyl-7-(3- methylpiperidine-1- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 3-methyl- piperidine (CAS 626-56- 2) 
                 1 
                 F 
                 378 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 0.80-0.98 (m, 4 H)  1.33-1.67 (m, 4 H)  1.68-1.86 (m, 4 H)  1.90-2.05 (m, 4 H)  2.18-2.29 (m, 1 H)  2.40-2.54 (m, 2 H)  2.54-2.63 (m, 1 H)  3.64-3.77 (m, 2 H)  4.24-4.38 (m, 1 H) 6.00  (br. s., 2 H) 7.95 (d, J = 3  Hz, 1 H) 8.19 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                 103 
                 6-amino-5-cyclohexyl- N-[2-(1,3-thiazol-2- yl)ethyl]-5H- pyrrolo[2,3- b]pyrazine-7- sulfonamide 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(thiazol-2- yl)-ethanamine (CAS 18453- 07-1) 
                 1 
                 D 
                 407 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.29-1.56 (m, 3 H)  1.76-1.85 (m, 1 H)  1.89-2.04 (m, 4 H)  2.38-2.54 (m, 2 H)  3.17-3.26 (m, 2 H)  3.34-3.46 (m, 2 H)  4.19-4.35 (m, 1 H)  5.82-6.05 (m, 3 H) 7.20  (d, J = 3 Hz, 1 H) 7.64 (d,  J = 3 Hz, 1 H) 7.95 (d,  J = 3 Hz, 1 H) 8.12 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
               
                 104 
                 8-{6-amino-5- cyclohexyl-5H- pyrrolo[2,3- b]pyrazine-7- sulfonyl}-8- azabicyclo[3.2.1]octan- 3-ol 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 8-azabicyclo- [3.2.1]octan-3- ol 
                 1 
                 D 
                 406 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.33-1.58 (m, 6 H)  1.76-1.85 (m, 3 H)  1.86-1.94 (m, 2 H)  1.95-2.05 (m, 4 H)  2.17-2.25 (m, 2 H)  2.37-2.53 (m, 2 H)  4.05-4.15 (m, 1 H)  4.24-4.40 (m, 3 H) 5.96  (br. s., 2 H) 7.95 (d, J = 3  Hz, 1 H) 8.22 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                 105 
                 5-cyclohexyl-7-[4- (2,2,2-trifluoroethyl)- piperazine-1-sulfonyl]- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 1-(2,2,2- trifluoroethyl)- piperazine (CAS 13349- 90-1) 
                 1 
                 Reverse  phase C18  5-95% water  (+0.05%  NH 3 )/MeOH 
                 447 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.34-1.55 (m, 3 H)  1.78-1.86 (m, 1 H)  1.89-2.05 (m, 4 H)  2.39-2.52 (m, 2 H)  2.73-2.83 (m, 4 H)  2.94-3.05 (m, 2 H)  3.19-3.31 (m, 4 H)  4.23-4.37 (m, 1 H) 5.94  (br. s., 2 H) 7.97 (d, J = 3  Hz, 1 H) 8.19 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                 106 
                 (1-{6-amino-5- cyclohexyl-5H- pyrrolo[2,3- b]pyrazine-7- sulfonyl}piperidin-4- yl)methanol 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 piperidin-4-yl- methanol (CAS 6457-49- 4) 
                 1 
                 Reverse  phase C18  5-95% water  (+0.05%  NH 3 )/MeOH 
                 394 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) 1.31-1.52 (m, 6 H)  1.77-1.84 (m, 3 H)  1.90-2.04 (m, 4 H)  2.38-2.53 (m, 2 H)  2.56-2.66 (m, 3 H)  3.42-3.51 (m, 2 H)  3.83-3.91 (m, 2 H)  4.23-4.35 (m, 1 H) 5.92  (br. s., 2 H) 7.95 (d, J = 3  Hz, 1 H) 8.19 (d, J = 3  Hz, 1 H) 
               
               
                   
               
               
                 107 
                 5-cyclohexyl-7-[4- (cyclopropylmethoxy)  piperidine-1-sulfonyl]- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (cyclopropyl- methoxy)- piperidine (CAS 865106- 51-0) 
                 1 
                 Reverse  phase C18  5-95% water  (+0.05%  NH 3 )/MeOH 
                 434 
                 (400 MHz,  DICHLOROMETHANE- d 2 ) −0.05-0.07 (m, 2 H)  0.27-0.37 (m, 2 H)  0.77-0.90 (m, 1 H)  1.21-1.44 (m, 3 H)  1.50-1.59 (m, 2 H)  1.65-1.72 (m, 1 H)  1.74-1.90 (m, 6 H)  2.25-2.39 (m, 2 H)  2.87-2.94 (m, 2 H)  3.06-3.12 (m, 2 H)  3.20-3.29 (m, 1 H)  3.30-3.41 (m, 2 H)  4.12-4.25 (m, 1 H)  5.78-5.97 (m, 2 H) 7.84  (d, J = 3 Hz, 1 H) 8.06 (d,  J = 3 Hz, 1 H) 
               
               
                   
               
            
           
         
       
     
     Examples 108 to 118 (see Table 4 following) were prepared using the general procedure 6 described below. 
     Procedure 6 
     A solution of 2-(5-cyclohexyl-7-((4-methoxyphenyl)sulfonyl)-5H-pyrrolo[2,3-b]pyrazin-6-yl)isoindoline-1,3-dione (Intermediate 44; 56 mg, 0.108 mmol) and hydrazine monohydrate (11 μL, 0.22 mmol) in ethanol (1 mL) was stirred at 70° C. in a sealed tube for 1 h. The mixture was allowed to cool then (except where noted otherwise) diluted with DCM and filtered. The filtrate was concentrated in vacuo and the crude product was purified by column chromatography on silica with the indicated eluent, or by preparative reverse phase HPLC as indicated in the table to afford the title compound. 
     
       
         
           
               
             
               
                 TABLE 
               
             
            
               
                   
               
               
                 Reverse phase preparative HPLC methods 
               
            
           
           
               
               
               
            
               
                   
                   
                 Gradient (acetonitrile/water  
               
               
                   
                 Method 
                 (with 0.1% ammonia unless indicated)) 
               
               
                   
                   
               
               
                   
                 A 
                  5-25% 
               
               
                   
                 B 
                  5-40% 
               
               
                   
                 C 
                 10-50% 
               
               
                   
                 D 
                 20-60% 
               
               
                   
                 E 
                 30-70% 
               
               
                   
                 F 
                 40-80% 
               
               
                   
                 G 
                 55-95% 
               
               
                   
                 H 
                 30-70% (0.1% formic acid) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
             
               
                 TABLE 4 
               
               
                   
               
               
                 Ex- 
                   
                   
                   
                 Puri- 
                   
                   
               
               
                 am- 
                   
                   
                   
                 fication 
                 MS 
                   1 H NMR 
               
               
                 ple 
                 Compound Name 
                 Structure 
                 Starting material 
                 Method 
                 ES+ 
                 data δ ppm 
               
               
                   
               
             
            
               
                 108 
                 5-cyclohexyl-7-[(4- methoxybenzene)- sulfonyl]-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7-((4- methoxyphenyl)sulfonyl)- 5H-pyrrolo[2,3-b]pyrazin- 6-yl)isoindoline-1,3-dione (Intermediate 44) 
                 0-100% ethyl acetate/ petrol 
                 387 
                 (400 MHz, METHANOL-d 4 ) 1.32- 1.43 (m, 1 H) 1.45-1.57 (m, 2 H) 1.73- 1.83 (m, 3 H) 1.89-1.98 (m, 2 H) 2.52-2.64 (m, 2 H) 3.84 (s, 3 H) 4.27- 4.39 (m, 1 H) 6.99-7.06 (m, 2 H) 7.90 (d, J = 3 Hz, 1 H) 8.00-8.07 (m, 3 H) 
               
               
                   
               
               
                 109 
                 5-cyclohexyl-7- (cyclopropanesulfonyl)- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7- (cyclopropylsulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione (Intermediate 45) 
                 0-100% ethyl acetate/ petrol 
                 321 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 0.81-0.89 (m, 2 H) 1.20-1.46 (m, 5 H) 1.65-1.74 (m, 1 H) 1.78- 1.93 (m, 4 H) 2.25-2.43 (m, 2 H) 2.67-2.78 (m, 1 H) 4.09-4.23 (m, 1 H) 5.89 (br. s., 2 H) 7.82-7.89 (m, 1 H) 8.06-8.12 (m, 1 H) 
               
               
                   
               
               
                 110 
                 5-cyclohexyl-7-[(3- fluorobenzene)sulfonyl]- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7-((3- fluorophenyl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione (Intermediate 46) 
                 0-100% ethyl acetate/ petrol 
                 375 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.17-1.41 (m, 3 H) 1.61-1.70 (m, 1 H) 1.71-1.89 (m, 4 H) 2.23-2.39 (m, 2 H) 4.05-4.21 (m, 1 H) 6.13 (br. s., 2 H) 7.09-7.19 (m, 1 H) 7.34- 7.42 (m, 1 H) 7.74-7.91 (m, 3 H) 8.06-8.13 (m, 1 H) 
               
               
                   
               
               
                 111 
                 5-cyclohexyl-7-[(2- fluorobenzene)sulfonyl]- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7-((2- fluorophenyl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione (Intermediate 47) 
                 0-100% ethyl acetate/ petrol 
                 375 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.16-1.47 (m, 3 H) 1.64-1.72 (m, 1 H) 1.76-1.94 (m, 4 H) 2.25-2.46 (m, 2 H) 4.09-4.26 (m, 1 H) 6.17 (br. s., 2 H) 6.95-7.05 (m, 1 H) 7.19- 7.30 (m, 1 H) 7.40-7.50 (m, 1 H) 7.76-7.83 (m, 1 H)7.94-8.01 (m, 1 H) 8.07-8.14 (m, 1 H)  
               
               
                   
               
               
                 112 
                 5-cyclohexyl-7-[(3- methoxybenzene)- sulfonyl]-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7-((3- methoxyphenyl)sulfonyl)- 5H-pyrrolo[2,3-b]pyrazin- 6-yl)isoindoline-1,3-dione (Intermediate 48) 
                 H 
                 387 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.27-1.56 (m, 3 H) 1.75-1.81 (m, 1 H) 1.83-2.02 (m, 4 H) 2.35-2.49 (m, 2 H) 3.87 (s, 3 H) 4.20-4.31 (m, 1 H) 6.21 (br. s., 2 H) 7.05-7.12 (m, 1 H) 7.36-7.45 (m, 1 H) 7.69-7.73 (m, 1 H) 7.78-7.83 (m, 1 H) 7.95 (d, J = 3 Hz, 1 H) 8.22 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                 113 
                 4-{6-amino-5- cyclohexyl-5H- pyrrolo[2,3-b]pyrazine- 7-sulfonyl}benzonitrile 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-((5-cyclohexyl-6-(1,3- dioxoisoindolin-2-yl)-5H- pyrrolo[2,3-b]pyrazin-7- yl)sulfonyl)benzonitrile (Intermediate 49) 
                 H 
                 382 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.32-1.53 (m, 3 H) 1.76-1.83 (m, 1 H) 1.85-1.93 (m, 2 H) 1.94-2.05 (m, 2 H) 2.36-2.49 (m, 2 H) 4.18- 4.32 (m, 1 H) 6.20 (br. s., 2 H) 7.77- 7.84 (m, 2 H) 7.97 (d, J = 3 Hz, 1 H) 8.22 (d, J = 3 Hz, 1 H) 8.27-8.35 (m, 2 H) 
               
               
                   
               
               
                 114 
                 7-[(3-chloro-4- methoxybenzene)- sulfonyl]-5-cyclohexyl- 5H-pyrrolo[2,3- b]pyrazin-6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(7-((3-chloro-4- methoxyphenyl)sulfonyl)-5- cyclohexyl-5H-pyrrolo[2,3- b]pyrazin-6-yl)isoindoline- 1,3-dione (Intermediate 50)  
                 Note 1 
                 421 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.32-1.56 (m, 3 H) 1.73-2.02 (m, 5 H) 2.34-2.51 (m, 2 H) 3.95 (s, 3 H) 4.19-4.35 (m, 1 H) 6.26 (br. s., 2 H) 7.04 (d, J = 9 Hz, 1 H) 7.95 (d, J = 3 Hz, 1 H) 8.09-8.17 (m, 2 H) 8.20 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                 115 
                 5-cyclohexyl-7-(6- methoxypyridine-3- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7-((6- methoxypyridin-3- yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione (Intermediate 51) 
                 0-4% methanol/ DCM 
                 388 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.28-1.55 (m, 3 H) 1.79 (d, J = 12 Hz, 1 H) 1.85-2.03 (m, 4 H) 2.36- 2.52 (m, 2 H) 3.58 (s, 3 H) 4.19-4.33 (m, 1 H) 6.18 (br. s., 2 H) 6.47 (d, J = 10 Hz, 1 H) 7.82-7.90 (m, 1 H) 7.96 (d, J = 3 Hz, 1 H) 8.19 (d, J = 3 Hz, 1 H) 8.41 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                 116 
                 5-cyclohexyl-7-{[4- (trifluoromethoxy)- benzene]sulfonyl}-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7-((4- (trifluoromethoxy)phenyl) sulfonyl)-5H-pyrrolo[2,3- b]pyrazin-6-yl)isoindoline- 1,3-dione (Intermediate 52) 
                 F 
                 441 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.25-1.51 (m, 3 H) 1.71-2.01 (m, 5 H) 2.30-2.48 (m, 2 H) 4.12-4.33 (m, 1 H) 6.15 (br. s., 2 H) 7.31 (d, J = 8 Hz, 2 H) 7.92 (d, J = 3 Hz, 1 H) 8.13- 8.28 (m, 3 H) 
               
               
                   
               
               
                 117 
                 5-cyclohexyl-7-(2,3- dihydro-1,4- benzodioxine-6- sulfonyl)-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7-((2,3- dihydrobenzo[b][1,4]dioxin- 6-yl)sulfonyl)-5H- pyrrolo[2,3-b]pyrazin-6- yl)isoindoline-1,3-dione (Intermediate 53) 
                 E 
                 415 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.24-1.51 (m, 3 H) 1.71-1.80 (m, 1 H) 1.81-1.99 (m, 4 H) 2.30-2.46 (m, 2 H) 4.15-4.30 (m, 5 H) 6.12 (br. s., 2 H) 6.91 (d, J = 8 Hz, 1 H) 7.58- 7.65 (m, 2 H) 7.90 (d, J = 3 Hz, 1 H) 8.17 (d, J = 3 Hz, 1 H) 
               
               
                   
               
               
                 118 
                 5-cyclohexyl-7-{[4- (difluoromethoxy)- benzene]sulfonyl}-5H- pyrrolo[2,3-b]pyrazin- 6-amine 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2-(5-cyclohexyl-7-((4- (difluoromethoxy)phenyl)- sulfonyl)-5H-pyrrolo[2,3- b]pyrazin-6-yl)isoindoline- 1,3-dione (Intermediate 54) 
                 Note 2 
                 443 
                 (400 MHz, DICHLOROMETHANE- d 2 ) 1.26-1.52 (m, 3 H) 1.71-2.00 (m, 5 H) 2.27-2.47 (m, 2 H) 4.12-4.32 (m, 1 H) 6.19 (br. s., 2 H) 6.59 (t, J = 74 Hz, 1 H) 7.19 (d, J = 9 Hz, 2 H) 7.92 (d, J = 3 Hz, 1 H) 8.14-8.23 (m, 3 H) 
               
               
                   
               
               
                 Note 1 
               
               
                 Alternative workup: The mixture was filtered and the precipitate was washed with ethanol. The filtrate was diluted with ether then concentrated in vacuo and the crude material was purified by column chromatography (silica, 10-55% EtOAc/petroleum ether gradient) to afford the title compound. 
               
               
                 Note 2 
               
               
                 Alternative workup: The reaction mixture was evaporated and the residue was dissolved in ethyl acetate then washed with dilute NaOH(aq), water, and saturated brine. The organic phase was dried (H-frit) and concentrated to afford the title compound. 
               
            
           
         
       
     
     3. Biological Efficacy of Compounds of the Invention 
     Screening Protocol: 
     Ca-Flux Functional Assay: Determination of Agonist/Positive Allosteric Modulator (PAM) Activity 
     GPR43 agonist/PAM activity was determined by measuring changes in intracellular calcium levels using a Ca 2+  sensitive fluorescent dye. The changes in fluorescent signal were monitored by FLIPR (manufactured by Molecular Devices). GPR43 mediated increases in intracellular Ca 2+  concentration were readily detected upon activation with sodium acetate. Prior to the assay (24 hours), CHO-K1 Gα16 cells stably expressing human GPR43 were-seeded in cell culture medium in black, clear-bottom 384-well plates (Corning Inc) and grown overnight at 37° C., 5% CO 2 . On the day of the assay, cell culture media was removed and cells were loaded with Calcium 5 Dye (Molecular Devices) diluted in HBSS containing 25 mM HEPES, 2.5 mM Probenecid, 0.1% BSA for 1 hour at 37° C., 5% CO 2 . 10 point half log concentration response curves of sodium acetate from 10 mM were conducted prior to the testing of compounds to calculate the sodium acetate concentration that produces 20% of the maximal response (EC 20 ). Test compounds (at to point half log concentration response curves from 10 μM) were added in the presence of sodium acetate to achieve a final concentration that produces approximately 20% maximal response as calculated from the previous experiment. The changes in fluorescent signal were monitored by FLIPR upon addition of the compound/EC 20  sodium acetate mix. The EC 50  values were determined from ten point concentration response curves. Curves were generated using the average of two wells for each data point. 
     The above assay detects both GPR43 receptor agonists and positive allosteric modulators of the GPR43 receptor, without distinguishing between the two. Activity in either regard is useful in the treatment of conditions associated with GPR43 receptor activity. 
     Results: 
       
     
       
         
           
               
               
               
               
               
               
             
               
                   
               
               
                 Compound  
                 Mean 
                 Compound  
                 Mean 
                 Compound  
                 Mean 
               
               
                 of Example  
                 EC 50   
                 of Example  
                 EC 50   
                 of Example  
                 EC 50   
               
               
                 No. 
                 (nM) 
                 No. 
                 (nM) 
                 No. 
                 (nM) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 1 
                 261 
                 2 
                 218 
                 3 
                 122 
               
               
                 4 
                 908 
                 5 
                 195 
                 6 
                 301 
               
               
                 7 
                 172 
                 8 
                 118 
                 9 
                 1249 
               
               
                 10 
                 2298 
                 11 
                 753 
                 12 
                 671 
               
               
                 13 
                 129 
                 14 
                 138 
                 15 
                 381 
               
               
                 16 
                 337 
                 17 
                 559 
                   
                   
               
               
                 19 
                 1775 
                 20 
                 2359 
                 21 
                 282 
               
               
                 22 
                 167 
                 23 
                 1052 
                 24 
                 2586 
               
               
                 25 
                 1099 
                 26 
                 79 
                 27 
                 277 
               
               
                 28 
                 707 
                 29 
                 31 
                 30 
                 199 
               
               
                 31 
                 6936 
                 32 
                 689 
                 33 
                 2722 
               
               
                 34 
                 5985 
                 35 
                 1861 
                 36 
                 245 
               
               
                 37 
                 468 
                 38 
                 169 
                 39 
                 657 
               
               
                 40 
                 7377 
                 41 
                 585 
                 42 
                 2296 
               
               
                 43 
                 6985 
                 44 
                 1075 
                 45 
                 2892 
               
               
                 46 
                 5257 
                 47 
                 6486 
                 48 
                 3261 
               
               
                 49 
                 211 
                 50 
                 760 
                 51 
                 5998 
               
               
                 52 
                 822 
                 53 
                 275 
                 54 
                 1536 
               
               
                 55 
                 7169 
                 56 
                 1305 
                 57 
                 259 
               
               
                 58 
                 2235 
                 59 
                 792 
                 60 
                 1408 
               
               
                 61 
                 4316 
                 62 
                 303 
                 63 
                 7946 
               
               
                 64 
                 7131 
                 65 
                 210 
                 66 
                 327 
               
               
                 67 
                 830 
                 68 
                 1465 
                 69 
                 547 
               
               
                 70 
                 3276 
                 71 
                 8378 
                 72 
                 1795 
               
               
                 73 
                 488 
                 74 
                 321 
                 75 
                 1187 
               
               
                 76 
                 1528 
                 77 
                 290 
                 78 
                 337 
               
               
                 79 
                 5968 
                 80 
                 6066 
                 81 
                 1253 
               
               
                 82 
                 821 
                 83 
                 931 
                 84 
                 3288 
               
               
                 85 
                 1060 
                 86 
                 1715 
                 87 
                 1553 
               
               
                 88 
                 3145 
                 89 
                 658 
                 90 
                 6184 
               
               
                 91 
                 8352 
                 92 
                 440 
                 93 
                 5697 
               
               
                 94 
                 308 
                 95 
                 334 
                 96 
                 1955 
               
               
                 97 
                 173 
                 98 
                 2812 
                 99 
                 1551 
               
               
                 100 
                 3931 
                 101 
                 7031 
                 102 
                 258 
               
               
                 103 
                 471 
                 104 
                 3077 
                 105 
                 647 
               
               
                 106 
                 3052 
                 107 
                 320 
                 108 
                 78 
               
               
                 109 
                 2902 
                 110 
                 343 
                 111 
                 209 
               
               
                 112 
                 295 
                 113 
                 486 
                 114 
                 206 
               
               
                 115 
                 4564 
                 116 
                 1082 
                 117 
                 124 
               
               
                 118 
                 177 
               
               
                   
               
            
           
         
       
     
     It will be understood that the present invention has been described above by way of example only. The examples are not intended to limit the scope of the present invention. Various modifications and embodiments can be made without departing from the scope and spirit of the invention, which is defined by the following claims.