Patent Publication Number: US-2006009406-A1

Title: Vectors having both isoforms of beta-hexosaminidase

Description:
I. ACKNOWLEDGMENTS  
      This application claims priority to U.S. Provisional Application No. 60/377,503 filed on May 2, 2002 for Vectors Having Both Isoforms of β-hexosamidase, and this application is a continuation of International Application No. PCT/US03/13672, filed May 2, 2003. Both of these applications are herein incorporated by reference in their entirety. 
    
    
     II. BACKGROUND OF THE INVENTION  
      Lysosomal storage disorders are disorders that typically arise from the aberrant or non-existent proteins involved in degradation function within the lysosomes. This causes a decrease in the lysosomal activity, which in turn causes an accumulation of unwanted materials in the cell. These unwanted materials can cause severe cellular toxicity and can impair, for example, neuronal function. These diseases severely impair the quality of life of those who have them, and can even result in death. Two diseases, Tay-Sachs and Sandoffs, are related to the functional impairment of the lysosomal protein β-hexosaminidase. β-hexosaminidase is a hetero or homo dimer made up of two subunits arising from two separate genes, HexA and HexB. Mutation of the HexA gene, causing functional problems with the HEX-α (HexA/HexB) polypeptide, results in Tay-Sachs disease, whereas mutation of the HexB gene, causing functional problems in the HEX-α (HexA/HexB) and HEX-β (HexB/HexB) polypeptides, results in Sandhoff&#39;s disease. Clinically, it is not uncommon for patients to display only mild features at infancy, but due to increasing lysosomal storage over time, progress to severe forms of the disease by adolescence.  
      Current treatments include bone marrow transplantation, which has been employed in some cases of individuals during childhood but with modest outcomes. A significant problem with the bone marrow transplantation approach is that it may address the lack of specific metabolic activity in peripheral tissues, but due to the presence of the blood-brain-barrier it fails to avert disease progression in the central nervous system. Hence patients often continue to clinically deteriorate due to central nervous system involvement with subsequent development of neurodegeneration, blindness, mental retardation, paralysis and dementia.  
      Enzyme replacement strategies targeting peripheral and central nervous system tissues utilizing gene therapy is a logical approach for treating inherited metabolic disorders. In a study by Akli et al. (1996), the authors report successful restoration of β-hexosaminidase in fibroblasts derived from patients with HexA deficiency via adenoviral-mediated gene transfer in vitro. Likewise a HexA transgene and a HexB transgene was successfully introduced into neural progenitor cells utilizing retroviral vectors (Lacorazza et al., “Expression of human beta-hexosaminidase alpha-subunit gene (the gene defect of Tay-Sachs disease) in mouse brains upon engraftment of transduced progenitor cells”  Nat Med  2(4):424-9 (1996 April).  
      Disclosed herein are vectors and methods which solve the problems associated with enzyme replacement therapies directed to β-hexosaminidase deficiencies.  
     III. SUMMARY OF THE INVENTION  
      In accordance with the purposes of this invention, as embodied and broadly described herein, this invention, in one aspect, relates to vector constructs that comprise sequence encoding the HEX-β polypeptide. Also disclosed are vector constructs comprising sequence encoding the HEX-β and the HEX-α polypeptides. Also disclosed are vectors for perinatal gene delivery, including delivery of HEX-α and HEX-β, which can be used for inherited lysosomal disorders such as Tay-Sachs and Sandoffs disease.  
      Additional advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The advantages of the invention will be realized and attained by means of the elements and combinations particularly pointed out in the appended claims. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed. 
    
    
     IV. BRIEF DESCRIPTION OF THE DRAWINGS  
      The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate several embodiments of the invention and together with the description, serve to explain the principles of the invention.  
       FIG. 1  shows that HEXlacZ encodes for both isoforms of human β-hexosaminidase, HexA &amp; HexB.  FIG. 1 (A) shows pHEXlacZ vector. BHK HexlacZ  are developed by stable HexlacZ transduction.  FIG. 3 (B) shows cells stain positively by X-gal histochemistry.  FIG. 3 (C) shows HexA &amp; HexB mRNA is detected by RT-PCR in total RNA extracts.  FIG. 3 (D 1 ) shows human HEXA &amp;  FIG. 3 (E 1 ) shows human HEXB proteins are detected in BHK HexlacZ  by imunocytochemistry.  FIG. 3 (F 1 ) shows HEXA &amp; HEXA+HEXB activity is measured by 4MUGS &amp; 4MUG fluorometry, respectively. FIG. (G) β-hexosaminidase detection by Fast Garnet histochemistry. (D 2 ,E 2 , G 2  are controls for D 1 ,E 1 ,G 1 , respectively).  
       FIG. 2  shows that the β-Hex therapeutic gene cross-corrects. An important property of the β-Hex transgene is the products hHEXA &amp; hHEXB have the ability to cross-correct, specifically, to be released extracellularly and then to be absorbed via paracrine pathways by other cells whereby they contribute to β-hexosaminidase activity. For this purpose, BHK HexlacZ  cells were cultured and the supernatant was collected (conditioned medium), filtered (0.45 mm) and applied on normal mouse kidney fibroblasts in culture. Forty-eight hours later, the cells were washed thoroughly with phosphate buffered saline, and briefly treated with a trypsin solution to remove extracellular proteins from the cell surfaces. Following trypsin inactivation with Tris/EDTA buffer, the cells were fixed with 4% paraformaldehyde solution and processed by Fast Garnet histochemistry for β-hexosaminidase activity. Fast Garnet histochemistry of murine fibroblasts exposed to (A) conditioned medium collected from BHK HexlacZ  cells compared to cells exposed to medium from normal parent BHK-21 cells (B). These results demonstrate that hHEXA &amp; hHEXB, products of the β-Hex transgene, are released into the extracellular medium and can be absorbed by other cells via paracrine pathways resulting in induction of the cellular β-hexosaminidase.  
       FIG. 3  shows a representation of a lentiviral system containing the HexA and HexB genes. The 3-vector FIV(Hex) system The FIV(Hex) lentiviral system is comprised of 3 vectors: Packaging vector providing the packaging instructions in trans-VSV-G envelop vector providing the envelop instructions in trans, -FIV(Hex) vector containing the therapeutic bicistronic gene.  
       FIG. 4  shows a representation of a Fiv(Hex) vector. Backbone FIV vector constructed by Proeschla et al. (1998)  
       FIG. 5  shows restriction fragment pattern of Feline immunodeficiency viral vector comprising a β-Hex construct. A maxi prep of FIV(Hex) clone 6.2 in 500 TB with 3× solution run through 2 columns. Yield of DNA was 1.095 mg. Final concentration is 1 microg/microl. Restriction enzyme digest with ScaI, not 1, Sal1, and Xho1. The bands are as expected.  
       FIG. 6  shows fibroblast infection by FIV( Hex) in vitro.  
       FIG. 7  shows an FIV(Hex) titration experiment.  
       FIG. 8  shows FIV(lacZ) administration to adult mice. FIV(lacZ) infection of murine fibroblasts (CrfK&#39;s) in vitro, as well as of liver cells following direct transdermal intra-hepatic injection. Liver, brain and spleen sections stained for β-galactosidase following intraperitoneal injection to 3 month old mice. lacZ expression was detected by X-gal staining (blue stain) and immunocytochemistry (ICC; black stain) on fixed tissue sections harvested 1 month post-treatment.  
       FIG. 9  shows FIV(lacZ) administration to P4 mice. Liver, brain, spleen and kidney sections stained for β-galactosidase following intraperitoneal injection to mice of perinatal age (4 days old). lacZ expression was detected by X-gal staining (blue stain) on fixed tissue sections harvested 3 months post-treatment.  
       FIG. 10  shows dose response of IP injections. Young adult mice (6 weeks old) were injected intra-peritoneally with different doses of FIV(lacZ) {0.1 mL, 0.5 mL, 1.0 mL and 2.0 mL of 103 infectious particles per mL} viral solution. One month following treatment the animals were sacrificed and lacZ reporter gene expression was measured. It was found that increasing doses of FIV result in increasing levels of gene therapy efficacy. In the clinical, human disease arena, this would optimally translate into intravenous administration of 10 −5 -10 −6  infectious FIV particles to ensure similar efficacy levels of gene therapy.  
       FIG. 11  shows diagrams of the vectors used to make the constructs discussed in Examples I and 2. FIV(Hex) is constructed by ligating the backbone part of FIV(LacZ), and the fragment of HexB-IRES-HexA from pHexLacZ. FIV(LacZ) is 12750 bp, after cut with SstII and NotI (generate 4500 bp and 8250 bp bands). Purify the 8250 bp band which contains the FIV backbone with CMV promoter. pHexlacZ is a construct of 10150 bp. Cut with NheI and NotI, there are 4700 bp and 5450 bp fragments. The 4700 bp band contains the structure of HexB-IES-HexA, which doesn&#39;t have CMV.  
       FIG. 12  shows how the structure of FIV(Hex) was confirmed. The constructs were digested with different restriction enzymes: (Result see  FIG. 5 ). Scal: cut once in the FIV backbone (generated one band 13 Kb). NotI: the site of ligation, and it is the only site (generated one band 13 Kb). Sal I: one site in HexB-IRFS-HexA and 3 sites in FIV backbone (generated one band t 8.5 Kb, one wide band with 2184 bp and 2400 bp, one band 34 bp which is invisible). Xho I: there is one site in HexB-IRFS-HexA and six sites in the FIV backbone (FIV(LacZ): at 502, 1410, 1453, 7559, 7883 and 9949 bp). These generated 6 bands (908 bp, 43 bp(invisible), 1.7Kb, 324 bp, 2066 bp, 3.3 Kb, and 2.8 Kb).  
       FIG. 13  shows a transcription termination cassette (STOP) flanked by 2 loxP sites was inserted between the promoter CMV and the therapeutic gene HexB-IRES-HexA. This results in inhibition of gene expression, until the STOP cassette is exsionally removed via the action of cre recombinase. The termination stop can consist of for example, a neomycin gene, whose termination signal acts as a termination signal for the rest of the transcript. Any reporter gene could be inserted and used in this way.  
       FIG. 14  shows a dually regulated inducible cre-recombinase system which was constructed. The activity of this construct is regulated exogenously by RU486. Furthermore, a stable cell line for this system was developed, whereby addition of RU486 in the culture media results in activation of cre-recombinase and subsequently excisional recombination of DNA, such as a transcription termination cassette flanked by 2 loxP sites.  
       FIG. 15  shows an example of the function of stable cell line, named GLVP/CrePr cell line, described in  FIG. 14 . In this case, the dual reporter vector CMV-lox-Luc-lox-AP was transiently transfected into the cell line. Alkaline phospatase (AP) activity was evaluated in vitro after the addition of RU486 to the culture media by an AP histochemical staining method.  
       FIG. 16A  shows the excisionally activated β-hexosaminidase gene Hex XAT  was constructed by placing a floxed transcription termination cassette (STOP) upstream to the first open reading frame: CMV-loxP-STOP-loxP-HexB-IRES-HexA.  FIG. 16B  shows Hex XAT  was transiently transfected into our inducible cre cell line. Activation of cre-recombinase resulted in loxP directed DNA recombination and excision of the STOP cassette.  FIG. 16C  Cre-mediated activation of HexXAT resulted in HexA and HexB upregulation (column 1). RU486 stimulation of GLVP/CrePr results in site-directed recombination and subsequent activation of a dormant transcriptional unit. A. shows the p Hex XAT , a bicistronic transgene comprised of a “floxed” transcription-termination cassette (STOP), and both isoforms of the human β-hexosaminidase, was transiently transfected into the GLVP/CrePr cell line. B. RU-486 administration resulted in loxP-directed excisional recombination, C. resulting in transcriptional activation and synthesis of HexA and HexB mRNA.  
       FIG. 17  shows the semi-quantitative analysis for HexA and HexB showed induction of gene transcription following HexXAT activation at (A) the mRNA level, (B) enzyme activity level in vitro, as well as (C) histochemical level in situ. RU486 significantly induces β-hexosaminidase expression in the GLVP/CrePr cell line. β-hexosaminidase activity was found significantly upregulated in p Hex XAT -transfected GLVP/CrePr cells 4 days after RU486 administration at the (A) HexA &amp; HexB mRNA, (B) enzyme activity in vitro, as well as (C) in fixed monolayers in situ, as assessed by RTPCR, 4-MUG fluorescence and X-Hex histochemistry, respectively.  
       FIG. 18  shows Hex XAT  was stably expressed in fibroblasts derived from a patient with Tay-Sachs disease (TSD). Gene activation was mediated by infection of the cells with a HSV aplicon viral vector capable of transducing cells with the cre recombinase. This figure demonstrates that activation of the Hex gene results in protection of the TSD cells from death following GM 2  substrate challenge.  
       FIG. 19  shows that the virus produced in  FIG. 3  above can resolve GM2 storage in TSD cells cultured in vitro.  
       FIG. 20  shows the Hex gene was cloned in the FIV backbone as shown in  FIG. 3  producing the virus FIV(Hex), which was then used to infect TSD cells challenged with GM 2  substrate. This figures shows that delivery of our Hex gene with FIV(Hex) in TSD cells in vitro confers protection to cell death following GM 2  administration.  
       FIG. 21  shows HexB −/−  knock out pups (2 days) were injected 100 uL of FIV(Hex) virus intraperitoneally. The animals were monitored weekly while they assumed growth until sacrificed (16-18 weeks of age).  
       FIG. 22  shows expression of HEXB protein in adult mice that were injected with the FIV(Hex) virus as infants 2 days after birth. HEXB protein expression was detected by immunocytochemistry in the liver and brain of these mice.  
       FIG. 23  shows locomotive performance in relation to age (in weeks) of 6 mice that were treated 2 days after birth: 3 mice were injected with FIV(Hex) and 3 with FIV(lacZ) and served as controls. At 16 weeks of age, the “classic” stage that the hexB knockout mice display the disease, there was significant disease difference between the two groups. 
    
    
     V. DETAILED DESCRIPTION  
      The present invention may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the Examples included therein and to the Figures and their previous and following description.  
      Before the present compounds, compositions, articles, devices, and/or methods are disclosed and described, it is to be understood that this invention is not limited to specific synthetic methods, specific recombinant biotechnology methods unless otherwise specified, or to particular reagents unless otherwise specified, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting.  
      Disclosed are the components to be used to prepare the disclosed compositions as well as the compositions themselves to be used within the methods disclosed herein. These and other materials are disclosed herein, and it is understood that when combinations, subsets, interactions, groups, etc. of these materials are disclosed that while specific reference of each various individual and collective combinations and permutation of these compounds may not be explicitly disclosed, each is specifically contemplated and described herein. For example, if a particular β-Hex vector is disclosed and discussed and a number of modifications that can be made to a number of molecules including the β-Hex vector are discussed, specifically contemplated is each and every combination and permutation of the β-Hex vector and the modifications that are possible unless specifically indicated to the contrary. Thus, if a class of molecules A, B, and C are disclosed as well as a class of molecules D, E, and F and an example of a combination molecule, A-D is disclosed, then even if each is not individually recited each is individually and collectively contemplated meaning combinations, A-E, A-F, B-D, B-E, B-F, C-D, C-E, and C-F are considered disclosed. Likewise, any subset or combination of these is also disclosed. Thus, for example, the sub-group of A-E, B-F, and C-E would be considered disclosed. This concept applies to all aspects of this application including, but not limited to, steps in methods of making and using the disclosed compositions. Thus, if there are a variety of additional steps that can be performed it is understood that each of these additional steps can be performed with any specific embodiment or combination of embodiments of the disclosed methods.  
     A. DEFINITIONS  
      As used in the specification and the appended claims, the singular forms “a,” “an” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a pharmaceutical carrier” includes mixtures of two or more such carriers, and the like.  
      Ranges may be expressed herein as from “about” one particular value, and/or to “about” another particular value. When such a range is expressed, another embodiment includes from the one particular value and/or to the other particular value. Similarly, when values are expressed as approximations, by use of the antecedent “about,” it will be understood that the particular value forms another embodiment. It will be further understood that the endpoints of each of the ranges are significant both in relation to the other endpoint, and independently of the other endpoint. It also understood that for every value disclosed, “about” that value is also disclosed. For example, if the value “10” is disclosed, then “about 10” is also disclosed, even if not specifically recited out as “about 10.” 
      In this specification and in the claims which follow, reference will be made to a number of terms which shall be defined to have the following meanings:  
      “Optional” or “optionally” means that the subsequently described event or circumstance may or may not occur, and that the description includes instances where said event or circumstance occurs and instances where it does not.  
      “Primers” are a subset of probes which are capable of supporting some type of enzymatic manipulation and which can hybridize with a target nucleic acid such that the enzymatic manipulation can occur. A primer can be made from any combination of nucleotides or nucleotide derivatives or analogs available in the art which do not interfere with the enzymatic manipulation.  
      “Probes” are molecules capable of interacting with a target nucleic acid, typically in a sequence specific manner, for example through hybridization. The hybridization of nucleic acids is well understood in the art and discussed herein. Typically a probe can be made from any combination of nucleotides or nucleotide derivatives or analogs available in the art.  
      Throughout this application, various publications are referenced. The disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains. The references disclosed are also individually and specifically incorporated by reference herein for the material contained in them that is discussed in the sentence in which the reference is relied upon.  
     B. Compositions and Methods  
     1. Lysosomal Disorders  
      Lysosomal storage disorders are a group of closely related metabolic diseases resulting from deficiency in enzymes essential for the degradation of gangliosides, mucopolysaccharides, as well as other complex macromolecules. With the dysfunction of a lysosomal enzyme, catabolism of correlate substrates remains incomplete, leading to accumulation of insoluble complex macromolecules within the lysosomes. For example, β-hexosaminidase defects result in lysosomal storage of GM 2  gangliosides leading to the development of Tay-Sachs or Sandhoffs disease. Similarly, mucopolysaccharidoses (MPS) are a group of closely related metabolic disorders that result from deficiencies in lysosomal enzymes involved in glycosaminoglycan metabolism, leading to lysosomal mucopolysaccharide storage. Affected patients, depending on the specific disorder and clinical severity, may present with neurodegeneration, mental retardation, paralysis, dementia and blindness, dysostosis multiplex, craniofacial malformations and facial dysfiguration. Below, some of the most common conditions of this family of diseases are summarized.  
     Representative Examples of Common Lysosomal Storage Disorders  
     
       
         
           
               
               
               
             
               
                   
               
               
                   
               
               
                 Disease 
                 Enzyme Deficiency 
                 Storage Metabolite 
               
               
                   
               
             
            
               
                 Glycogenosis-Type 
                 α-1,4-Glucosidase 
                 Glycogen 
               
               
                 2 
               
               
                 Gangliosidoses 
               
               
                 GM 1  Gangliosidosis 
                 GM 1  ganglioside β-galactosidase 
                 GM 1  ganglioside 
               
               
                 Tay-Sachs disease 
                 Hexosaminidase - α subunit 
                 GM 2  ganglioside 
               
               
                 Sandhoff disease 
                 Hexosaminidase - β subunit 
                 GM 2  ganglioside 
               
               
                 Sulfatidoses 
               
               
                 Krabbe disease 
                 Galactosylceramidase 
                 galactocerebroside 
               
               
                 Fabry disease 
                 α-Galactosidase A 
                 ceramide trihexoside 
               
               
                 Gaucher disease 
                 Glucocerebrosidase 
                 glucocerebroside 
               
               
                 Niemann-Pick - 
                 Sphingomyelinase 
                 sphingomyelin 
               
               
                 types A &amp; B 
               
               
                 Mucopolysaccharidoses 
               
               
                 Hurler&#39;s syndrome 
                 α-L-Iduronidase 
                 dermatan/heparan sulfate 
               
               
                 Hunter&#39;s syndrome 
                 L-Iduronosulfate sulfatase 
               
               
                 Mucolipidoses 
               
               
                 Mucolipidosis - II 
                 Mannose-6-phosphate kinases 
                 mucopolysaccharide/ 
               
               
                 Pseudo-Hurler&#39;s 
                   
                 glycolipid 
               
               
                 Fucosidosis 
                 α-Fucosidase 
                 Glycoproteins 
               
               
                 Mannosidosis 
                 a-Mannosidase 
                 oligosaccharides 
               
               
                 Wolman Disease 
                 Acid Lipase 
                 triglycerides 
               
               
                   
               
            
           
         
       
     
     2. Histopathology &amp; Pathophysiology—A Progressive Disorder  
      In storage diseases, the affected cells become distended and display vacuolated cytoplasms, which appear as swollen lysosomes under the electronic microscope. For example, in the central nervous system, the neurons of the brain, trigeminal and spinal root ganglia in patients suffering from GM 2  gangliodisoses display swollen vacuolated perikarya stored with excessive amounts of lysosomal storage. As a result, these organelles become large in size and numbers, interfering with normal cell functions. The formation of meganeurites, axon hillock enlargements accompanied by secondary neuritic sprouting, present as cardinal histopathological feature of gangliosidoses and mucopolysaccharidoses (Purpura and Suzuki, 1976; Walkley et al., 1988). Purpura and Suzuki proposed that meganeurites, and the synapses they develop, contribute to the onset and progression of neuronal dysfunction in storage diseases, by altering electrical properties of neurons and modifying integrative operations of somatodendritic synaptic inputs. In addition, Walkley et al. (1991) suggested that this neuroaxonal dystrophy commonly involved GABAergic neurons, and proposed that the resulting defect in neurotransmission in inhibitory circuits may be an important factor underlying brain dysfunction in lysosomal storage diseases. Consequently, the clinical phenotype often includes neurodegeneration, mental retardation, paralysis, dementia and blindness. In addition, some storage disorders also affect peripheral tissues, such as cartilage and bone, resulting in abnormal growth &amp; development of long bones, vertebrae, ribs and jaws, ultimately leading to anomalies of the skeleton, the cranium and dysfiguration of the face (Mucopolysaccharidoses, and Sandhoff&#39;s disease to some degree).  
      One cardinal characteristic of storage disorders is their progressively worsening (progressive) nature. The deficiency of metabolic enzymes results in accumulation of insoluble metabolites in the lysosomes, which becomes excessive and deleterious over time due to the additive effects of accumulating insoluble metabolite storage. For example, patients suffering from mucopolysaccharidoses (Hurler&#39;s or Hunter&#39;s) display only a mild degree of the disease&#39;s phenotype at infancy, but, due to increasing storage over time, progress to severe forms by adolescence, often leading to death (Gorlin et al., 1990). This provides a window of opportunity in mammalian development during which the pathophysiological process of the disease can be attenuated by restoring lysosomal enzymatic activity early enough in life to prevent the development of a “full-blown” disease and, perhaps, to reverse its progression.  
     3. Tay-Sachs &amp; Sandhoffs Disorders  
      The lysosomal enzyme β-hexosaminidase is comprised of 2 subunits (peptides), HEX-α and HEX-β, encoded by two distinct genes, HexA and HexB, respectively. β-hexosaminidase exists in 3 isoforms (proteins), HEXA (α/β heterodimer), HEXB (β/β homodimer) and HEXS (α/α homodimer). Mutation of the HexA gene, causing functional problems with the HEX-α polypeptide in humans results in Tay Sachs disease, whereas mutation of the HexB gene, causing functional problems in the β-Hex polypeptide, in Sandhoff&#39;s disease. In Tay Sachs disease, HexA mutation results in loss of HEXA isoform (α/β heterodimer), whereas in Sandhoff&#39;s disease, HexB mutation results in loss of both HEXA (α/β heterodimer) and HexB (β/β homodimer) isoforms, leading to a more severe clinical phenotype. Affected patients, depending on the clinical severity, may present with neurodegeneration, mental and motor deteriotation, dysarthria, impaired thermal sensitivity, blindness, as well as facial dysfiguration (doll-like and coarse facies). Histopathologically, the cells of the brain (neurons and glia), spleen and cartilage appear swollen with vaculolated/clear perikarya suggestive of lysosomal storage. Biochemical analysis reveals a complete lack of β-hexosaminidase activity accompanied by lysosomal accumulation of GM 2  gangliosides. As a result, the lysosomes become large in size and numbers, significantly crippling normal cellular function. Clinically, it is not uncommon for patients to display only mild features at infancy, but due to increasing storage over time, progress to severe forms of the disease by adolescence (Gorlin et al., 1990). Similarly, other affected mammals, such as affected mice pups, display only mild anomalies at birth, but quickly develop their distinct abnormal features (1 month of age).  
     4. Blood Brain Barrier Formation  
      The blood-brain barrier (BBB) is a structure unique to the central nervous system and is the result of tight junctions between the brain endothelial cells (Goldstein et al., 1986). Previous work (Risau et al., 1986) on the development of mouse BBB using large protein molecules (horse radish peroxidase) suggested BBB formation during the late days of embryonic life (E17 in mouse). Furthermore, BBB in the adult is not absolute, whereby certain areas of the brain do not develop BBB and thus allow for free exchange of molecules through them. These areas include the median eminence (hypothalamus), pituitary, choroids plexus, pineal gland, subfomical organ, organum vasculosum lamina terminalis and area posterma (Risau &amp; Wolburg, 1990). This allows for the intrusion of FIV(Hex) virions into the brain matter through an incomplete BBB as well as through areas lacking BBB during the first few days after birth as discussed in the examples herein. Disclosed herein a diffuse expression of lacZ throughout the brain of P4 mice injected with FIV(lacZ) versus periventricular only localization following “adult administration” was shown.  
     5. Immune System Development  
      Specific immunity in vertebrates is dependent on the host&#39;s ability to generate a heterogeneous repertoire of antigen-binding structures that are displayed on the surface of lymphocytes. Immunologic competence arises early in mammalian development. Since the expression of β-Hex therapeutic gene in hexA −/− /hexB −/−  mice may be perceived as presentation of “non-self” antigens, one needs to consider the possibility of an immune response against human HEXA and HEXB following gene therapy. In these terms, perinatal administration can offer a unique opportunity in gene therapy application. Specifically, numerous studies have documented that the human and mouse neonate is unable to mount satisfactory responses to various antigenic challenges, which in many instances is delayed well beyond infancy (Schroeder et al., 1995). Therefore, due to this “immature” immunologic state of mice and humans early in their postnatal life, perinatal gene therapy is consistent with adequate “training” of the immune system to recognize HEXA and HEXB as “self” antigens circumventing any potential immunologic rejection. It is understood that the transtherapy can take place in an infant as well.  
      Disclosed are nucleic acids comprising sequence encoding HEX-α and sequence encoding HEX-β. Also disclosed are nucleic acids, wherein the nucleic acid further comprises an IRES sequence, wherein the nucleic acids express more than one IRES sequence, wherein the vectors express an IRES sequence after each Hex nucleics acid, wherein the nucleic acid further comprises a promoter sequence, wherein the nucleic acid further comprises a promoter sequence, wherein the HEX-β has at least 80% identity to the sequence set forth in SEQ ID NO:3 and the HEX-α has at least 80% identity to the sequence set forth in SEQ ID NO:1, wherein the HEX-β has at least 85% identity to the sequence set forth in SEQ ID NO:3 and the HEX-α has at least 80% identity to the sequence set forth in SEQ ID NO:1, wherein the HEX-β has at least 90% identity to the sequence set forth in SEQ ID NO:3 and the HEX-α has at least 80% identity to the sequence set forth in SEQ ID NO:1, wherein the HEX-β has at least 95% identity to the sequence set forth in SEQ ID NO:3 and the HEX-α has at least 80% identity to the sequence set forth in SEQ ID NO:1, wherein the HEX-β has the sequence set forth in SEQ ID NO:3 and the HEX-α has the sequence set forth in SEQ ID NO:1, wherein the sequence encoding the HEX-β is orientated 5′ to the sequence encoding HEX-α, wherein the sequence encoding the HEX-β is orientated 5′ to the RES sequence and the RES sequence is located 5′ to the sequence encoding HEX-α, wherein the promoter is located 5′ to the sequence encoding the HEX-β and the sequence encoding the HEX-β is orientated 5′ to the IRES sequence and the RES sequence is located 5′ to the sequence encoding HEX-α.  
      Also disclosed are vectors comprising the disclosed nucleic acids. Also disclosed are cells comprising the disclosed nucleic acids and vectors.  
      Also disclosed are non-human mammal comprising the disclosed nucleic acids, vectors, and cells disclosed herein.  
      Also disclosed are methods of providing HEX-α in a cell comprising transfecting the cell with the nucleic acids, also disclosed are methods of providing HEX-β in a cell comprising transfecting the cell with the nucleic acids, also disclosed are method of providing HEX-α and HEX-β in a cell comprising transfecting the cell with the nucleic acid of claims  1 - 4 .  
      Also disclosed are method of delivering the disclosed compositions, wherein the transfection occurs in vitro or in vivo.  
      Disclosed are methods of making a transgenic organism comprising administering the disclosed nucleic acids, vectors and/or cells.  
      Disclosed are methods of making a transgenic organism comprising transfecting a lentiviral vector to the organism at during a perinatal stage of the organism&#39;s development.  
      Also disclosed are methods of treating a subject having Tay Sachs disease and/or Sandoff disease comprising administering any of the disclosed compounds and compositions.  
     C. Compositions  
     1. β-Hexosaminidase Transgene (β-Hex)  
      The β-Hexosaminidase protein is a protein comprised of two subunits, one subunit is encoded by the HexA gene and a second subunit encoded by the gene HexB. The human HexA Exon 1 can be found 316 bp upstream of MstII site; chromosome 15q11-15qter. The human HexA gene can be found at human chromosomal region 15q23—q24. The human HexB gene can be found on chromosome 5, map 5q13.  
      Disclosed are constructs capable of expressing both the HexA gene product and the HexB gene product, from a single construct. Any construct capable of expressing both the HexA and HexB gene products is referred to as a β-Hex construct herein. The β-Hex construct allows for synthesis of all β-hexosaminidase protein isoforms, HEXA (α/β heterodimer), HEXB (β/β homodimer) and HEXS (α/α homodimer). Disclosed are nucleic acid constructs comprising a cytomegalovirus (CMV) promoter-driven bicistronic gene (β-Hex) that encodes for both human HexA and HexB genes, which can lead to the synthesis of functional β-hexosaminidase isoenzymes.  
      The β-Hex construct typically comprises four parts: 1) a promoter, 2) the HexA coding sequence, 3) the HexB coding sequence, and 4) an IRES sequence (integrated ribosomal entry site). These four parts can be integrated into any vector delivery system. In preferred embodiments, the orientation of the four parts is 5′-promoter-HexB-IRES-HexA-3′.  
      The promoter can be any promoter, such as those discussed herein. It is understood as discussed herein that there are functional variants of the HexA and HexB which can be made. Furthermore, it is understood that that there are functional variants of the IRES element, for example as discussed herein. Typically the genes to be expressed are placed on either side of the IRES sequence.  
      The IRES element is an internal ribosomal entry sequence which can be iosolated from the encephalomyocarditis crius (ECMV). This element allows multiple genes to be expressed and correctly translated when the genes are on the same construct. IRES sequences are discussed in for example, U.S. Pat. No: 4,937,190 which is herein incorporated by reference at least for material related to IRES sequences and their use.  
      HexA and HexB cDNA can be obtained from the American Tissue Culture Collection. (American Tissue Culture Collection, Manassas, Va. 20110-2209; Hex-α: ATCC# 57206; Hex-β ATCC# 57350) The IRES sequence can be obtained from a number of sources including commercial sources, such as the pIRES expressing vector from Clonetech (Clontech, Palo Alto Calif. 94303-4230).  
      Also disclosed are tricistronic constructs encoding for both isoforms of human β-hexosaminidase, hHexA &amp; hHexB, as well as the β-galactosidase reporter gene (lacZ).  
      Global delivery of the disclosed constructs is also disclosed. Disclosed is a pseudotyped feline immunodeficiency virus (FIV) for global β-Hex delivery. Stable expression of the therapeutic gene aids prolonged restoration of the genetic anomaly enhancing treatment efficacy and contributing to long-term therapeutic outcomes. The backbone FIV system has been shown to effectively incorporate, due to its lentiviral properties, the transgene of interest into the host&#39;s genome, allowing for stable gene expression (Poeschla et al., 1998). Disclosed herein is stable expression of the reporter gene lacZ for over 3 months in mice following perinatal systemic FIV(lacZ) administration.  
      A model system for the study of these vectors is a mouse that is knockout mouse deficient in both HexA and HexB, since the hexA −/− /hexB −/−  mouse is characterized by global disruption of the hexA and hexB genes. Gene disruption in this mouse is global, and therefore, can be used as a model for global replacement. The timing of gene therapy is important as it is closely related to the temporal development of the disorder. HexA −/− /hexB −/−  mice display mild phenotype aberrations at birth and quickly develop craniofacial dysplasia by 4-5 weeks of age. Similarly, it is not uncommon for patients suffering from this class of genetic disorders to display only mild degree of the disease at infancy, and to progress to severe forms by adolescence.  
     2. Delivery of the Compositions to Cells  
      Delivery can be applied, in general, via local or systemic routes of administration. Local administration includes virus injection directly into the region or organ of interest, versus intravenous (IV) or intraperitoneal (IP) injections (systemic) aiming at viral delivery to multiple sites and organs via the blood circulation. Previous research on the effects of local administration demonstrated gene expression limited to the site/organ of the injection, which did not extend to the rest of the body (Daly et al., 1999a; Kordower et al., 1999). Furthermore, previous studies have demonstrated successful global gene transfer to multiple tissues and organs in rodents and primates following viral IV and IP injections (Daly et al., 1999b; Tamtal et al., 2001; McCormack et al., 2001; Lipschutz et al., 2001). Disclosed herein IP injection of FIV(lacZ) in mice of adult (3 months old) as well as of perinatal age (P4) resulted in global transfer and expression of the reporter gene lacZ in brain, liver, spleen and kidney. Also disclosed, the levels of expression achieved via IP injections were superior to those acquired following local administration directly into the liver.  
      There are a number of compositions and methods which can be used to deliver nucleic acids to cells, either in vitro or in vivo. These methods and compositions can largely be broken down into two classes: viral based delivery systems and non-viral based delivery systems. For example, the nucleic acids can be delivered through a number of direct delivery systems such as, electroporation, lipofection, calcium phosphate precipitation, plasmids, viral vectors, viral nucleic acids, phage nucleic acids, phages, cosmids, or via transfer of genetic material in cells or carriers such as cationic liposomes. Appropriate means for transfection, including viral vectors, chemical transfectants, or physico-mechanical methods such as electroporation and direct diffusion of DNA, are described by, for example, Wolff, J. A., et al., Science, 247, 1465-1468, (1990); and Wolff, J. A. Nature, 352, 815-818, (1991)Such methods are well known in the art and readily adaptable for use with the compositions and methods described herein. In certain cases, the methods will be modified to specifically function with large DNA molecules. Further, these methods can be used to target certain diseases and cell populations by using the targeting characteristics of the carrier.  
     a) Nucleic Acid Based Delivery Systems  
      Transfer vectors can be any nucleotide construction used to deliver genes into cells (e.g., a plasmid), or as part of a general strategy to deliver genes, e.g., as part of recombinant retrovirus or adenovirus (Ram et al. Cancer Res. 53:83-88, (1993)).  
      As used herein, plasmid or viral vectors are agents that transport the disclosed nucleic acids, such as the β-Hex construct into the cell without degradation and include a promoter yielding expression of the HexA and HexB encoding sequences in the cells into which it is delivered. In some embodiments the vectors for the β-Hex constructs are derived from either a virus, retrovirus, or lentivirus. Viral vectors can be, for example, Adenovirus, Adeno-associated virus, Herpes virus, Vaccinia virus, Polio virus, AIDS virus, neuronal trophic virus, Sindbis and other RNA viruses, including these viruses with the HIV backbone, and lentiviruses. Also preferred are any viral families which share the properties of these viruses which make them suitable for use as vectors. Retroviruses include Murine Maloney Leukemia virus, MMLV, and retroviruses that express the desirable properties of MMLV as a vector. Retroviral vectors are able to carry a larger genetic payload, i.e., a transgene, such as, the disclosed β-Hex constructs or marker gene, than other viral vectors, and for this reason are a commonly used vector. However, they are not as useful in non-proliferating cells. Adenovirus vectors are relatively stable and easy to work with, have high titers, and can be delivered in aerosol formulation, and can transfect non-dividing cells. Pox viral vectors are large and have several sites for inserting genes, they are thermostable and can be stored at room temperature. A preferred embodiment is a viral vector, which has been engineered so as to suppress the immune response of the host organism, elicited by the viral antigens. Preferred vectors of this type will carry coding regions for Interleukin 8 or 10.  
      Viral vectors can have higher transaction (ability to introduce genes) abilities than chemical or physical methods to introduce genes into cells. Typically, viral vectors contain, nonstructural early genes, structural late genes, an RNA polymerase III transcript, inverted terminal repeats necessary for replication and encapsidation, and promoters to control the transcription and replication of the viral genome. When engineered as vectors, viruses typically have one or more of the early genes removed and a gene or gene/promotor cassette is inserted into the viral genome in place of the removed viral DNA. Constructs of this type can carry up to about 8 kb of foreign genetic material. The necessary functions of the removed early genes are typically supplied by cell lines which have been engineered to express the gene products of the early genes in trans.  
     (1) Retroviral Vectors  
      A retrovirus is an animal virus belonging to the virus family of Retroviridae, including any types, subfamilies, genus, or tropisms. Retroviral vectors, in general, are described by Verma, I. M., Retroviral vectors for gene transfer. In Microbiology-1985, American Society for Microbiology, pp. 229-232, Washington, (1985), which is incorporated by reference herein. Examples of methods for using retroviral vectors for gene therapy are described in U.S. Pat. Nos. 4,868,116 and 4,980,286; PCT applications WO 90/02806 and WO 89/07136; and Mulligan, (Science 260:926-932 (1993)); the teachings of which are incorporated herein by reference.  
      A retrovirus is essentially a package which has packed into it nucleic acid cargo. The nucleic acid cargo carries with it a packaging signal, which ensures that the replicated daughter molecules will be efficiently packaged within the package coat. In addition to the package signal, there are a number of molecules which are needed in cis, for the replication, and packaging of the replicated virus. Typically a retroviral genome, contains the gag, pol, and env genes which are involved in the making of the protein coat. It is the gag, pol, and env genes which are typically replaced by the foreign DNA that it is to be transferred to the target cell. Retrovirus vectors typically contain a packaging signal for incorporation into the package coat, a sequence which signals the start of the gag transcription unit, elements necessary for reverse transcription, including a primer binding site to bind the tRNA primer of reverse transcription, terminal repeat sequences that guide the switch of RNA strands during DNA synthesis, a purine rich sequence 5′ to the 3′ LTR that serve as the priming site for the synthesis of the second strand of DNA synthesis, and specific sequences near the ends of the LTRs that enable the insertion of the DNA state of the retrovirus to insert into the host genome. The removal of the gag, pol, and env genes allows for about 8 kb of foreign sequence to be inserted into the viral genome, become reverse transcribed, and upon replication be packaged into a new retroviral particle. This amount of nucleic acid is sufficient for the delivery of a one to many genes depending on the size of each transcript. It is preferable to include either positive or negative selectable markers along with other genes in the insert.  
      Since the replication machinery and packaging proteins in most retroviral vectors have been removed (gag, pol, and env), the vectors are typically generated by placing them into a packaging cell line. A packaging cell line is a cell line which has been transfected or transformed with a retrovirus that contains the replication and packaging machinery, but lacks any packaging signal. When the vector carrying the DNA of choice is transfected into these cell lines, the vector containing the gene of interest is replicated and packaged into new retroviral particles, by the machinery provided in cis by the helper cell. The genomes for the machinery are not packaged because they lack the necessary signals.  
     (2) Adenoviral Vectors  
      The construction of replication-defective adenoviruses has been described (Berkner et al., J. Virology 61:1213-1220 (1987); Massie et al., Mol. Cell. Biol. 6:2872-2883 (1986); Haj-Ahmad et al., J. Virology 57:267-274 (1986); Davidson et al., J. Virology 61:1226-1239 (1987); Zhang “Generation and identification of recombinant adenovirus by liposome-mediated transfection and PCR analysis” BioTechniques 15:868-872 (1993)). The benefit of the use of these viruses as vectors is that they are limited in the extent to which they can spread to other cell types, since they can replicate within an initial infected cell, but are unable to form new infectious viral particles. Recombinant adenoviruses have been shown to achieve high efficiency gene transfer after direct, in vivo delivery to airway epithelium, hepatocytes, vascular endothelium, CNS parenchyma and a number of other tissue sites (Morsy, J. Clin. Invest. 92:1580-1586 (1993); Kirshenbaum, J. Clin. Invest. 92:381-387 (1993); Roessler, J. Clin. Invest. 92:1085-1092 (1993); Moullier, Nature Genetics 4:154-159 (1993); La Salle, Science 259:988-990 (1993); Gomez-Foix, J. Biol. Chem. 267:25129-25134 (1992); Rich, Human Gene Therapy 4:461-476 (1993); Zabner, Nature Genetics 6:75-83 (1994); Guzman, Circulation Research 73:1201-1207 (1993); Bout, Human Gene Therapy 5:3-10 (1994); Zabner, Cell 75:207-216 (1993); Caillaud, Eur. J. Neuroscience 5:1287-1291 (1993); and Ragot, J. Gen. Virology 74:501-507 (1993)). Recombinant adenoviruses achieve gene transduction by binding to specific cell surface receptors, after which the virus is internalized by receptor-mediated endocytosis, in the same manner as wild type or replication-defective adenovirus (Chardonnet and Dales, Virology 40:462-477 (1970); Brown and Burlingham, J. Virology 12:386-396 (1973); Svensson and Persson, J. Virology 55:442-449 (1985); Seth, et al., J. Virol. 51:650-655 (1984); Seth, et al., Mol. Cell. Biol. 4:1528-1533 (1984); Varga et al., J. Virology 65:6061-6070 (1991); Wickham et al., Cell 73:309-319 (1993)).  
      A viral vector can be one based on an adenovirus which has had the E1 gene removed and these virons are generated in a cell line such as the human 293 cell line. In another preferred embodiment both the E1 and E3 genes are removed from the adenovirus genome.  
     (3) Adeno-Asscociated Viral Vectors  
      Another type of viral vector is based on an adeno-associated virus (AAV). This defective parvovirus is a preferred vector because it can infect many cell types and is nonpathogenic to humans. AAV type vectors can transport about 4 to 5 kb and wild type AAV is known to stably insert into chromosome 19. Vectors which contain this site specific integration property are preferred. An especially preferred embodiment of this type of vector is the P4.1 C vector produced by Avigen, San Francisco, Calif., which can contain the herpes simplex virus thymidine kinase gene, HSV-tk, and/or a marker gene, such as the gene encoding the green fluorescent protein, GFP.  
      In another type of AAV virus, the AAV contains a pair of inverted terminal repeats (ITRs) which flank at least one cassette containing a promoter which directs cell-specific expression operably linked to a heterologous gene. Heterologous in this context refers to any nucleotide sequence or gene which is not native to the AAV or B19 parvovirus.  
      Typically the AAV and B19 coding regions have been deleted, resulting in a safe, noncytotoxic vector. The AAV ITRs, or modifications thereof, confer infectivity and site-specific integration, but not cytotoxicity, and the promoter directs cell-specific expression. U.S. Pat. No. 6,261,834 is herein incorproated by reference for material related to the AAV vector.  
      The vectors of the present invention thus provide DNA molecules which are capable of integration into a mammalian chromosome without substantial toxicity.  
      The inserted genes in viral and retroviral usually contain promoters, and/or enhancers to help control the expression of the desired gene product. A promoter is generally a sequence or sequences of DNA that function when in a relatively fixed location in regard to the transcription start site. A promoter contains core elements required for basic interaction of RNA polymerase and transcription factors, and may contain upstream elements and response elements.  
     (4) Lentiviral Vectors  
      The vectors can be lentiviral vectors, including but not limited to, SIV vectors, HIV vectors or a hybrid construct of these vectors, including viruses with the HIV backbone. These vectors also include first, second and third generation lentiviruses. Third generation lentiviruses have lentiviral packaging genes split into at least 3 independent plasmids or constructs. Also vectors can be any viral family that share the properties of these viruses which make them suitable for use as vectors. Lentiviral vectors are a special type of retroviral vector which are typically characterized by having a long incubation period for infection. Furthermore, lentiviral vectors can infect non-dividing cells. Lentiviral vectors are based on the nucleic acid backbone of a virus from the lentiviral family of viruses. Typically, a lentiviral vector contains the 5′ and 3′ LTR regions of a lentivirus, such as SIV and HIV. Lentiviral vectors also typically contain the Rev Responsive Element (RRE) of a lentivirus, such as SIV and HIV.  
     (a) Feline Immunodeficiency Viral Vectors  
      One type of vector that the disclosed constructs can be delivered in is the VSV-G pseudotyped Feline Immunodeficiency Virus system developed by Poeschla et al. (1998). This lentivirus has been shown to efficiently infect dividing, growth arrested as well as post-mitotic cells. Furthermore, due to its lentiviral properties, it allows for incorporation of the transgene into the host&#39;s genome, leading to stable gene expression. This is a 3-vector system, whereby each confers distinct instructions: the FIV vector carries the transgene of interest and lentiviral apparatus with mutated packaging and envelope genes. A vesicular stomatitis virus G-glycoprotein vector (VSV-G; Burns et al., 1993) contributes to the formation of the viral envelope in trans. The third vector confers packaging instructions in trans (Poeschla et al., 1998). FIV production is accomplished in vitro following co-transfection of the aforementioned vectors into 293-T cells. The FIV-rich supernatant is then collected, filtered and can be used directly or following concentration by centrifugation. Titers routinely range between 10 4 -10 7  bfu/ml.  
     (5) Packaging Vectors  
      As discussed above, retroviral vectors are based on retroviruses which contain a number of different sequence elements that control things as diverse as integration of the virus, replication of the integrated virus, replication of un-integrated virus, cellular invasion, and packaging of the virus into infectious particles. While the vectors in theory could contain all of their necessary elements, as well as an exogenous gene element (if the exogenous gene element is small enough) typically many of the necessary elements are removed. Since all of the packaging and replication components have been removed from the typical retroviral, including lentiviral, vectors which will be used within a subject, the vectors need to be packaged into the initial infectious particle through the use of packaging vectors and packaging cell lines. Typically retroviral vectors have been engineered so that the myriad functions of the retrovirus are separated onto at least two vectors, a packaging vector and a delivery vector. This type of system then requires the presence of all of the vectors providing all of the elements in the same cell before an infectious particle can be produced. The packaging vector typically carries the structural and replication genes derived from the retrovirus, and the delivery vector is the vector that carries the exogenous gene element that is preferably expressed in the target cell. These types of systems can split the packaging functions of the packaging vector into multiple vectors, e.g., third-generation lentivirus systems. Dull, T. et al., “A Third-generation lentivirus vector with a conditional packaging system” J. Virol 72(11):8463-71 (1998)  
      Retroviruses typically contain an envelope protein (env). The Env protein is in essence the protein which surrounds the nucleic acid cargo. Furthermore cellular infection specificity is based on the particular Env protein associated with a typical retrovirus. In typical packaging vector/delivery vector systems, the Env protein is expressed from a separate vector than for example the protease (pro) or integrase (in) proteins.  
     (6) Packaging Cell Lines  
      The vectors are typically generated by placing them into a packaging cell line. A packaging cell line is a cell line which has been transfected or transformed with a retrovirus that contains the replication and packaging machinery, but lacks any packaging signal. When the vector carrying the DNA of choice is transfected into these cell lines, the vector containing the gene of interest is replicated and packaged into new retroviral particles, by the machinery provided in cis by the helper cell. The genomes for the machinery are not packaged because they lack the necessary signals. One type of packaging cell line is a 293 cell line.  
     (7) Large Payload Viral Vectors  
      Molecular genetic experiments with large human herpesviruses have provided a means whereby large heterologous DNA fragments can be cloned, propagated and established in cells permissive for infection with herpesviruses (Sun et al., Nature genetics 8: 33-41, 1994; Cotter and Robertson, Curr Opin Mol Ther 5: 633-644, 1999). These large DNA viruses (herpes simplex virus (HSV) and Epstein-Barr virus (EBV), have the potential to deliver fragments of human heterologous DNA &gt;150 kb to specific cells. EBV recombinants can maintain large pieces of DNA in the infected B-cells as episomal DNA. Individual clones carried human genomic inserts up to 330 kb appeared genetically stable The maintenance of these episomes requires a specific EBV nuclear protein, EBNA1, constitutively expressed during infection with EBV. Additionally, these vectors can be used for transfection, where large amounts of protein can be generated transiently in vitro. Herpesvirus amplicon systems are also being used to package pieces of DNA &gt;220 kb and to infect cells that can stably maintain DNA as episomes.  
      Other useful systems include, for example, replicating and host-restricted non-replicating vaccinia virus vectors.  
     b) Non-Nucleic Acid Based Systems  
      The disclosed compositions can be delivered to the target cells in a variety of ways. For example, the compositions can be delivered through electroporation, or through lipofection, or through calcium phosphate precipitation. The delivery mechanism chosen will depend in part on the type of cell targeted and whether the delivery is occurring for example in vivo or in vitro.  
      Thus, the compositions can comprise, in addition to the disclosed constructs or vectors for example, lipids such as liposomes, such as cationic liposomes (e.g., DOTMA, DOPE, DC-cholesterol) or anionic liposomes. Liposomes can further comprise proteins to facilitate targeting a particular cell, if desired. Administration of a composition comprising a compound and a cationic liposome can be administered to the blood afferent to a target organ or inhaled into the respiratory tract to target cells of the respiratory tract. Regarding liposomes, see, e.g., Brigham et al.  Am. J. Resp. Cell. Mol. Biol.  1:95-100 (1989); Felgner et al.  Proc. Natl. Acad. Sci USA  84:7413-7417 (1987); U.S. Pat. No.4,897,355. Furthermore, the compound can be administered as a component of a microcapsule that can be targeted to specific cell types, such as macrophages, or where the diffusion of the compound or delivery of the compound from the microcapsule is designed for a specific rate or dosage.  
      In the methods described above which include the administration and uptake of exogenous DNA into the cells of a subject (i.e., gene transduction or transfection), delivery of the compositions to cells can be via a variety of mechanisms. As one example, delivery can be via a liposome, using commercially available liposome preparations such as LIPOFECTIN, LIPOFECTAMINE (GIBCO-BRL, Inc., Gaithersburg, Md.), SUPERFECT (Qiagen, Inc. Hilden, Germany) and TRANSFECTAM (Promega Biotec, Inc., Madison, Wis.), as well as other liposomes developed according to procedures standard in the art. In addition, the nucleic acid or vector of this invention can be delivered in vivo by electroporation, the technology for which is available from Genetronics, Inc. (San Diego, Calif.) as well as by means of a SONOPORATION machine (ImaRx Pharmaceutical Corp., Tucson, Ariz.).  
      The materials may be in solution, suspension (for example, incorporated into microparticles, liposomes, or cells). These may be targeted to a particular cell type via antibodies, receptors, or receptor ligands. The following references are examples of the use of this technology to target specific proteins to tumor tissue (Senter, et al.,  Bioconjugate Chem.,  2:447-451, (1991); Bagshawe, K. D.,  Br. J. Cancer,  60:275-281, (1989); Bagshawe, et al.,  Br. J. Cancer,  58:700-703, (1988); Senter, et al.,  Bioconjugate Chem.,  4:3-9, (1993); Battelli, et al.,  Cancer Immunol. Immunother.,  35:421-425, (1992); Pietersz and McKenzie,  Inmunolog. Reviews,  129:57-80, (1992); and Roffler, et al.,  Biochem. Pharmacol,  42:2062-2065, (1991)). These techniques can be used for a variety of other specific cell types. Vehicles such as “stealth” and other antibody conjugated liposomes (including lipid mediated drug targeting to colonic carcinoma), receptor mediated targeting of DNA through cell specific ligands, lymphocyte directed tumor targeting, and highly specific therapeutic retroviral targeting of murine glioma cells in vivo. The following references are examples of the use of this technology to target specific proteins to tumor tissue (Hughes et al.,  Cancer Research,  49:6214-6220, (1989); and Litzinger and Huang, Biochimica et  Biophysica Acta,  1104:179-187, (1992)). In general, receptors are involved in pathways of endocytosis, either constitutive or ligand induced. These receptors cluster in clathrin-coated pits, enter the cell via clathrin-coated vesicles, pass through an acidified endosome in which the receptors are sorted, and then either recycle to the cell surface, become stored intracellularly, or are degraded in lysosomes. The internalization pathways serve a variety of functions, such as nutrient uptake, removal of activated proteins, clearance of macromolecules, opportunistic entry of viruses and toxins, dissociation and degradation of ligand, and receptor-level regulation. Many receptors follow more than one intracellular pathway, depending on the cell type, receptor concentration, type of ligand, ligand valency, and ligand concentration. Molecular and cellular mechanisms of receptor-mediated endocytosis has been reviewed (Brown and Greene,  DNA and Cell Biology  10:6, 399-409 (1991)).  
      Nucleic acids that are delivered to cells which are to be integrated into the host cell genome, typically contain integration sequences. These sequences are often viral related sequences, particularly when viral based systems are used. These viral intergration systems can also be incorporated into nucleic acids which are to be delivered using a non-nucleic acid based system of deliver, such as a liposome, so that the nucleic acid contained in the delivery system can be come integrated into the host genome.  
      Other general techniques for integration into the host genome include, for example, systems designed to promote homologous recombination with the host genome. These systems typically rely on sequence flanking the nucleic acid to be expressed that has enough homology with a target sequence within the host cell genome that recombination between the vector nucleic acid and the target nucleic acid takes place, causing the delivered nucleic acid to be integrated into the host genome. These systems and the methods necessary to promote homologous recombination are known to those of skill in the art.  
     c) In Vivo/Ex Vivo  
      As described herein, the compositions can be administered in a pharmaceutically acceptable carrier and can be delivered to the subjects cells in vivo and/or ex vivo by a variety of mechanisms well known in the art (e.g., uptake of naked DNA, liposome fusion, intramuscular injection of DNA via a gene gun, endocytosis and the like).  
      If ex vivo methods are employed, cells or tissues can be removed and maintained outside the body according to standard protocols well known in the art. The compositions can be introduced into the cells via any gene transfer mechanism, such as, for example, calcium phosphate mediated gene delivery, electroporation, microinjection or proteoliposomes. The transduced cells can then be infused (e.g., in a pharmaceutically acceptable carrier) or homotopically transplanted back into the subject per standard methods for the cell or tissue type. Standard methods are known for transplantation or infusion of various cells into a subject.  
      If in vivo delivery methods are performed the methods can be designed to deliver the nucleic acid constructs directly to a particular cell type, via any delivery mechanism, such as intra-peritoneal injection of a vector construct. In this type of delivery situation, the nucleic acid constructs can be delivered to any type of tissue, for example, brain or neural or muscle. The nucleic acid constructs can also be delivered such that they generally deliver the nucleic acid constructs to more than one type of cell. This type of delivery can be accomplished, by for example, injecting the constructs intraperitoneally into the flank of the organism. (See Example 2 and  FIGS. 8-10 ). It in certain delivery methods, the timing of the delivery is monitored. For example, the nucleic acid constructs can be delivered at the perinatal stage of the recipients life or at the adult stage.  
      The disclosed compositions, can be delivered to any type of cell. For example, they can be delivered to any type of mammalian cell. Exemplary types of cells neuron, glia, fibroblast, chondrocyte, osteocyte, endothelial, and hepatocyte.  
     3. Expression Systems  
      The nucleic acids that are delivered to cells typically contain expression controlling systems. For example, the inserted genes in viral and retroviral systems usually contain promoters, and/or enhancers to help control the expression of the desired gene product. A promoter is generally a sequence or sequences of DNA that function when in a relatively fixed location in regard to the transcription start site. A promoter contains core elements required for basic interaction of RNA polymerase and transcription factors, and may contain upstream elements and response elements.  
     a) Viral Promoters and Enhancers  
      Preferred promoters controlling transcription from vectors in mammalian host cells may be obtained from various sources, for example, the genomes of viruses such as: polyoma, Simian Virus 40 (SV40), adenovirus, retroviruses, hepatitis-B virus and most preferably cytomegalovirus, or from heterologous mammalian promoters, e.g. beta actin promoter. The early and late promoters of the SV40 virus are conveniently obtained as an SV40 restriction fragment which also contains the SV40 viral origin of replication (Fiers et al.,  Nature,  273: 113 (1978)). The immediate early promoter of the human cytomegalovirus is conveniently obtained as a HindIII E restriction fragment (Greenway, P. J. et al.,  Gene  18: 355-360 (1982)). Of course, promoters from the host cell or related species also are useful herein.  
      Enhancer generally refers to a sequence of DNA that functions at no fixed distance from the transcription start site and can be either 5′ (Laimins, L. et al.,  Proc. Natl. Acad. Sci.  78: 993 (1981)) or 3′ (Lusky, M. L., et al.,  Mol. Cell Bio.  3: 1108 (1983)) to the transcription unit. Furthermore, enhancers can be within an intron (Banerji, J. L. et al.,  Cell  33: 729 (1983)) as well as within the coding sequence itself (Osborne, T. F., et al.,  Mol. Cell Bio.  4: 1293 (1984)). They are usually between 10 and 300 bp in length, and they function in cis. Enhancers function to increase transcription from nearby promoters. Enhancers also often contain response elements that mediate the regulation of transcription. Promoters can also contain response elements that mediate the regulation of transcription. Enhancers often determine the regulation of expression of a gene. While many enhancer sequences are now known from mammalian genes (globin, elastase, albumin, fetoprotein and insulin), typically one will use an enhancer from a eukaryotic cell virus for general expression. Preferred examples are the SV40 enhancer on the late side of the replication origin (bp 100-270), the cytomegalovirus early promoter enhancer, the polyoma enhancer on the late side of the replication origin, and adenovirus enhancers.  
      The promoter and/or enhancer may be specifically activated either by light or specific chemical events which trigger their function. Systems can be regulated by reagents such as tetracycline and dexamethasone. There are also ways to enhance viral vector gene expression by exposure to irradiation, such as gamma irradiation, or alkylating chemotherapy drugs.  
      In certain embodiments the promoter and/or enhancer region can act as a constitutive promoter and/or enhancer to maximize expression of the region of the transcription unit to be transcribed. In certain constructs the promoter and/or enhancer region be active in all eukaryotic cell types, even if it is only expressed in a particular type of cell at a particular time. A preferred promoter of this type is the CMV promoter (650 bases). Other preferred promoters are SV40 promoters, cytomegalovirus (full length promoter), and retroviral vector LTF.  
      It has been shown that all specific regulatory elements can be cloned and used to construct expression vectors that are selectively expressed in specific cell types such as melanoma cells. The glial fibrillary acetic protein (GFAP) promoter has been used to selectively express genes in cells of glial origin.  
      Expression vectors used in eukaryotic host cells (yeast, fungi, insect, plant, animal, human or nucleated cells) may also contain sequences necessary for the termination of transcription which may affect mRNA expression. These regions are transcribed as polyadenylated segments in the untranslated portion of the mRNA encoding tissue factor protein. The 3′ untranslated regions also include transcription termination sites. It is preferred that the transcription unit also contain a polyadenylation region. One benefit of this region is that it increases the likelihood that the transcribed unit will be processed and transported like mRNA. The identification and use of polyadenylation signals in expression constructs is well established. It is preferred that homologous polyadenylation signals be used in the transgene constructs. In certain transcription units, the polyadenylation region is derived from the SV40 early polyadenylation signal and consists of about 400 bases. It is also preferred that the transcribed units contain other standard sequences alone or in combination with the above sequences improve expression from, or stability of, the construct.  
      In certain embodiments the promoters are constitutive promoters. This can be any promoter that causes transcription regulation in the absence of the addition of other factors. Examples of this type of promoter are the CMV promoter and the beta actin promoter, as well as others dicussed herein. In certain embodiments the promoter can consist of fusions of one or more different types of promoters. For example, the regulatory regions of the CMV promoter and the beta actin promoter are well known and understood, examples, of which are disclosed herein. Parts of these promoters can be fused together to, for example, produce a CMV-beta actin fusion promoter, such as the one shown in SEQ ID NO:23. It is understood that this type of promoter has a CMV component and a beta actin component. These components can function independently as promoters, and thus, are themselves considered beta actin promoters and CMV promoters. A promoter can be any portion of a known promoter that causes promoter activity. It is well understood that many promoters, including the CMV and Beta Actin promoters have functional domains which are understood and that these can be used as a beta actin promoter or CMV promoter. Furthermore, these domains can be determined. For example, SEQ ID NO:s 21-41 display a number of CMV promoters, beta actin promoters, and fusion promoters. These promoters can be compared, and for example, functional regions delineated, as described herein. Furthermore, each of these sequences can function independently or together in any combination to provide a promoter region for the disclosed nucleic acids.  
     b) Markers  
      The viral vectors can include nucleic acid sequence encoding a marker product. This marker product is used to determine if the gene has been delivered to the cell and once delivered is being expressed. Preferred marker genes are the  E. Coli  lacZ gene, which encodes β-galactosidase, and green fluorescent protein. [105] In some embodiments the marker maybe a selectable marker. Examples of suitable selectable markers for mammalian cells are dihydrofolate reductase (DHFR), thymidine kinase, neomycin, neomycin analog G418, hydromycin, and puromycin. When such selectable markers are successfully transferred into a mammalian host cell, the transformed mammalian host cell can survive if placed under selective pressure. There are two widely used distinct categories of selective regimes. The first category is based on a cell&#39;s metabolism and the use of a mutant cell line which lacks the ability to grow independent of a supplemented media. Two examples are: CHO DHFR-cells and mouse LTK-cells. These cells lack the ability to grow without the addition of such nutrients as thymidine or hypoxanthine. Because these cells lack certain genes necessary for a complete nucleotide synthesis pathway, they cannot survive unless the missing nucleotides are provided in a supplemented media. An alternative to supplementing the media is to introduce an intact DHFR or TK gene into cells lacking the respective genes, thus altering their growth requirements. Individual cells which were not transformed with the DHFR or TK gene will not be capable of survival in non-supplemented media.  
      The second category is dominant selection which refers to a selection scheme used in any cell type and does not require the use of a mutant cell line. These schemes typically use a drug to arrest growth of a host cell. Those cells which have a novel gene would express a protein conveying drug resistance and would survive the selection. Examples of such dominant selection use the drugs neomycin, (Southern P. and Berg, P.,  J. Molec. Appl. Genet.  1: 327 (1982)), mycophenolic acid, (Mulligan, R. C. and Berg, P.  Science  209: 1422 (1980)) or hygromycin, (Sugden, B. et al.,  Mol. Cell. Biol.  5: 410-413 (1985)). The three examples employ bacterial genes under eukaryotic control to convey resistance to the appropriate drug G418 or neomycin (geneticin), xgpt (mycophenolic acid) or hygromycin, respectively. Others include the neomycin analog G418 and puramycin.  
     c) Post Transcriptional Regulatory Elements  
      The disclosed vectors can also contain post-transcriptional regulatory elements. Post-transcriptional regulatory elements can enhance mRNA stability or enhance translation of the transcribed mRNA. An exemplary post-transcriptional regulatory sequence is the WPRE sequence isolated from the woodchuck hepatitis virus. (Zufferey R, et al., “Woodchuck hepatitis virus post-transcriptional regulatory element enhances expression of transgenes delivered by retroviral vectors,”  J Virol;  73:2886-92 (1999)). Post-transcriptional regulatory elements can be positioned both 3′ and 5′ to the exogenous gene, but it is preferred that they are positioned 3′ to the exogenous gene.  
     d) Transduction Efficiency Elements  
      Transduction efficiency elements are sequences that enhance the packaging and transduction of the vector. These elements typically contain polypurine sequences. An example of a transduction efficiency element is the ppt-cts sequence that contains the central polypurine tract (ppt) and central terminal site (cts) from the HIV-1 pSG3 molecular clone (SEQ ID NO:1 bp 4327 to 4483 of HIV-1 pSG3 clone).  
     e) 3′ Untranslated Regions  
      Expression vectors used in eukaryotic host cells (yeast, fungi, insect, plant, animal, human or nucleated cells) may also contain sequences necessary for the termination of transcription which may affect mRNA expression. These 3′ untranslated regions are transcribed as polyadenylated segments in the untranslated portion of the mRNA encoding the exogenous gene. The 3′ untranslated regions also include transcription termination sites. The transcription unit also can contain a polyadenylation region. One benefit of this region is that it increases the likelihood that the transcribed unit will be processed and transported like mRNA. The identification and use of polyadenylation signals in expression constructs is well established. Homologous polyadenylation signals can be used in the transgene constructs. In an embodiment of the transcription unit, the polyadenylation region is derived from the SV40 early polyadenylation signal and consists of about 400 bases. Transcribed units can contain other standard sequences alone or in combination with the above sequences improve expression from, or stability of, the construct.  
     4. Sequence Similarities  
      It is understood that as discussed herein the use of the terms homology and identity mean the same thing as similarity. Thus, for example, if the use of the word homology is used between two non-natural sequences it is understood that this is not necessarily indicating an evolutionary relationship between these two sequences, but rather is looking at the similarity or relatedness between their nucleic acid sequences. Many of the methods for determining homology between two evolutionarily related molecules are routinely applied to any two or more nucleic acids or proteins for the purpose of measuring sequence similarity regardless of whether they are evolutionarily related or not.  
      In general, it is understood that one way to define any known variants and derivatives or those that might arise, of the disclosed genes and proteins herein, is through defining the variants and derivatives in terms of homology to specific known sequences. This identity of particular sequences disclosed herein is also discussed elsewhere herein. In general, variants of genes and proteins herein disclosed typically have at least, about 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99 percent homology to the stated sequence or the native sequence. Those of skill in the art readily understand how to determine the homology of two proteins or nucleic acids, such as genes. For example, the homology can be calculated after aligning the two sequences so that the homology is at its highest level.  
      Another way of calculating homology can be performed by published algorithms. Optimal alignment of sequences for comparison may be conducted by the local homology algorithm of Smith and Waterman Adv. Appl. Math. 2: 482 (1981), by the homology alignment algorithm of Needleman and Wunsch, J. MoL Biol. 48: 443 (1970), by the search for similarity method of Pearson and Lipman, Proc. Natl. Acad. Sci. U.S.A. 85: 2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by inspection.  
      The same types of homology can be obtained for nucleic acids by for example the algorithms disclosed in Zuker, M.  Science  244:48-52, 1989, Jaeger et al.  Proc. Natl. Acad. Sci. USA  86:7706-7710, 1989, Jaeger et al. Methods Enzymol. 183:281-306, 1989 which are herein incorporated by reference for at least material related to nucleic acid alignment. It is understood that any of the methods typically can be used and that in certain instances the results of these various methods may differ, but the skilled artisan understands if identity is found with at least one of these methods, the sequences would be said to have the stated identity, and be disclosed herein.  
      For example, as used herein, a sequence recited as having a particular percent homology to another sequence refers to sequences that have the recited homology as calculated by any one or more of the calculation methods described above. For example, a first sequence has 80 percent homology, as defined herein, to a second sequence if the first sequence is calculated to have 80 percent homology to the second sequence using the Zuker calculation method even if the first sequence does not have 80 percent homology to the second sequence as calculated by any of the other calculation methods. As another example, a first sequence has 80 percent homology, as defined herein, to a second sequence if the first sequence is calculated to have 80 percent homology to the second sequence using both the Zuker calculation method and the Pearson and Lipman calculation method even if the first sequence does not have 80 percent homology to the second sequence as calculated by the Smith and Waterman calculation method, the Needleman and Wunsch calculation method, the Jaeger calculation methods, or any of the other calculation methods. As yet another example, a first sequence has 80 percent homology, as defined herein, to a second sequence if the first sequence is calculated to have 80 percent homology to the second sequence using each of calculation methods (although, in practice, the different calculation methods will often result in different calculated homology percentages).  
     5. Hybridization/Selective Hybridization  
      The term hybridization typically means a sequence driven interaction between at least two nucleic acid molecules, such as a primer or a probe and a gene. Sequence driven interaction means an interaction that occurs between two nucleotides or nucleotide analogs or nucleotide derivatives in a nucleotide specific manner. For example, G interacting with C or A interacting with T are sequence driven interactions. Typically sequence driven interactions occur on the Watson-Crick face or Hoogsteen face of the nucleotide. The hybridization of two nucleic acids is affected by a number of conditions and parameters known to those of skill in the art. For example, the salt concentrations, pH, and temperature of the reaction all affect whether two nucleic acid molecules will hybridize.  
      Parameters for selective hybridization between two nucleic acid molecules are well known to those of skill in the art. For example, in some embodiments selective hybridization conditions can be defined as stringent hybridization conditions. For example, stringency of hybridization is controlled by both temperature and salt concentration of either or both of the hybridization and washing steps. For example, the conditions of hybridization to achieve selective hybridization may involve hybridization in high ionic strength solution (6×SSC or 6×SSPE) at a temperature that is about 12-25° C. below the Tm (the melting temperature at which half of the molecules dissociate from their hybridization partners) followed by washing at a combination of temperature and salt concentration chosen so that the washing temperature is about 5° C. to 20° C. below the Tm. The temperature and salt conditions are readily determined empirically in preliminary experiments in which samples of reference DNA immobilized on filters are hybridized to a labeled nucleic acid of interest and then washed under conditions of different stringencies. Hybridization temperatures are typically higher for DNA-RNA and RNA-RNA hybridizations. The conditions can be used as described above to achieve stringency, or as is known in the art. (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989; Kunkel et al. Methods Enzymol. 1987:154:367, 1987 which is herein incorporated by reference for material at least related to hybridization of nucleic acids). A preferable stringent hybridization condition for a DNA:DNA hybridization can be at about 68° C. (in aqueous solution) in 6×SSC or 6×SSPE followed by washing at 68° C. Stringency of hybridization and washing, if desired, can be reduced accordingly as the degree of complementarity desired is decreased, and further, depending upon the G-C or A-T richness of any area wherein variability is searched for. Likewise, stringency of hybridization and washing, if desired, can be increased accordingly as homology desired is increased, and further, depending upon the G-C or A-T richness of any area wherein high homology is desired, all as known in the art.  
      Another way to define selective hybridization is by looking at the amount (percentage) of one of the nucleic acids bound to the other nucleic acid. For example, in some embodiments selective hybridization conditions would be when at least about, 60, 65, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 percent of the limiting nucleic acid is bound to the non-limiting nucleic acid. Typically, the non-limiting primer is in for example, 10 or 100 or 1000 fold excess. This type of assay can be performed at under conditions where both the limiting and non-limiting primer are for example, 10 fold or 100 fold or 1000 fold below their kd, or where only one of the nucleic acid molecules is 10 fold or 100 fold or 1000 fold or where one or both nucleic acid molecules are above their k d .  
      Another way to define selective hybridization is by looking at the percentage of primer that gets enzymatically manipulated under conditions where hybridization is required to promote the desired enzymatic manipulation. For example, in some embodiments selective hybridization conditions would be when at least about, 60, 65, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 percent of the primer is enzymatically manipulated under conditions which promote the enzymatic manipulation, for example if the enzymatic manipulation is DNA extension, then selective hybridization conditions would be when at least about 60, 65, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 percent of the primer molecules are extended. Preferred conditions also include those suggested by the manufacturer or indicated in the art as being appropriate for the enzyme performing the manipulation.  
      Just as with homology, it is understood that there are a variety of methods herein disclosed for determining the level of hybridization between two nucleic acid molecules. It is understood that these methods and conditions may provide different percentages of hybridization between two nucleic acid molecules, but unless otherwise indicated meeting the parameters of any of the methods would be sufficient. For example if 80% hybridization was required and as long as hybridization occurs within the required parameters in any one of these methods it is considered disclosed herein.  
      It is understood that those of skill in the art understand that if a composition or method meets any one of these criteria for determining hybridization either collectively or singly it is a composition or method that is disclosed herein.  
     6. Nucleic Acids  
      There are a variety of molecules disclosed herein that are nucleic acid based, including for example the nucleic acids that encode, for example HexA and HexB, or functional nucleic acids. The disclosed nucleic acids can be made up of for example, nucleotides, nucleotide analogs, or nucleotide substitutes. Non-limiting examples of these and other molecules are discussed herein. It is understood that for example, when a vector is expressed in a cell, that the expressed mRNA will typically be made up of A, C, G, and U. Likewise, it is understood that if, for example, an antisense molecule is introduced into a cell or cell environment through for example exogenous delivery, it is advantagous that the antisense molecule be made up of nucleotide analogs that reduce the degradation of the antisense molecule in the cellular environment.  
      A nucleotide is a molecule that contains a base moiety, a sugar moiety and a phosphate moiety. Nucleotides can be linked together through their phosphate moieties and sugar moieties creating an internucleoside linkage. The base moiety of a nucleotide can be adenin-9-yl (A), cytosin-1-yl (C), guanin-9-yl (G), uracil-1-yl (U), and thymin-1-yl (T). The sugar moiety of a nucleotide is a ribose or a deoxyribose. The phosphate moiety of a nucleotide is pentavalent phosphate. An non-limiting example of a nucleotide would be 3′-AMP (3′-adenosine monophosphate) or 5′-GMP (5′-guanosine monophosphate).  
      A nucleotide analog is a nucleotide which contains some type of modification to either the base, sugar, or phosphate moieties. Modifications to nucleotides are well known in the art and would include for example, 5-methylcytosine (5-me-C), 5-hydroxymethyl cytosine, xanthine, hypoxanthine, and 2-aminoadenine as well as modifications at the sugar or phosphate moieties.  
      Nucleotide substitutes are molecules having similar functional properties to nucleotides, but which do not contain a phosphate moiety, such as peptide nucleic acid (PNA). Nucleotide substitutes are molecules that will recognize nucleic acids in a Watson-Crick or Hoogsteen manner, but which are linked together through a moiety other than a phosphate moiety. Nucleotide substitutes are able to conform to a double helix type structure when interacting with the appropriate target nucleic acid.  
      It is also possible to link other types of molecules (conjugates) to nucleotides or nucleotide analogs to enhance for example, cellular uptake. Conjugates can be chemically linked to the nucleotide or nucleotide analogs. Such conjugates include but are not limited to lipid moieties such as a cholesterol moiety. (Letsinger et al., Proc. Natl. Acad. Sci. USA, 1989,86, 6553-6556),  
      A Watson-Crick interaction is at least one interaction with the Watson-Crick face of a nucleotide, nucleotide analog, or nucleotide substitute. The Watson-Crick face of a nucleotide, nucleotide analog, or nucleotide substitute includes the C2, N1, and C6 positions of a purine based nucleotide, nucleotide analog, or nucleotide substitute and the C2, N3, C4 positions of a pyrimidine based nucleotide, nucleotide analog, or nucleotide substitute.  
      A Hoogsteen interaction is the interaction that takes place on the Hoogsteen face of a nucleotide or nucleotide analog, which is exposed in the major groove of duplex DNA. The Hoogsteen face includes the N7 position and reactive groups (NH2 or 0) at the C6 position of purine nucleotides.  
     a) Sequences  
      There are a variety of sequences related to the HexA, HexB, IRES sequences, and promoter sequences. For example, the HexA and hexB genes have the following Genbank Accession Numbers: M16411 and NM — 000520 for HexA and NM — 000521 for HexB, these sequences and others are herein incorporated by reference in their entireties as well as for individual subsequences contained therein. It is understood that there are numerous Genbank accession sequences related to HexA and HexB, all of which are incorporated by reference herein.  
      One particular sequence set forth in SEQ ID NO:4 and having Genbank accession number NM — 000521, which is a sequence for human HexB cDNA, is used herein, as an example, to exemplify the disclosed compositions and methods. It is understood that the description related to this sequence is applicable to any sequence related to HexA or HexB unless specifically indicated otherwise. Those of skill in the art understand how to resolve sequence discrepancies and differences and to adjust the compositions and methods relating to a particular sequence to other related sequences. Primers and/or probes can be designed for any of the sequences disclosed herein given the information disclosed herein and that known in the art.  
      It is also understood for example that there are numerous bicistronic vectors that can be used to create the β-Hex construct nucleic acids See for example, Genbank accession no Y11035 and Y11034.  
     b) Primers and Probes  
      Disclosed are compositions including primers and probes, which are capable of interacting with, for example, the β-Hex construct nucleic acids, as disclosed herein. In certain embodiments the primers are used to support DNA amplification reactions. Typically the primers will be capable of being extended in a sequence specific manner. Extension of a primer in a sequence specific manner includes any methods wherein the sequence and/or composition of the nucleic acid molecule to which the primer is hybridized or otherwise associated directs or influences the composition or sequence of the product produced by the extension of the primer. Extension of the primer in a sequence specific manner therefore includes, but is not limited to, PCR, DNA sequencing, DNA extension, DNA polymerization, RNA transcription, or reverse transcription. Techniques and conditions that amplify the primer in a sequence specific manner are preferred. In certain embodiments the primers are used for the DNA amplification reactions, such as PCR or direct sequencing. It is understood that in certain embodiments the primers can also be extended using non-enzymatic techniques, where for example, the nucleotides or oligonucleotides used to extend the primer are modified such that they will chemically react to extend the primer in a sequence specific manner. Typically the disclosed primers hybridize with, for example, the β-Hex construct nucleic acid, or region of the β-Hex construct nucleic acids or they hybridize with the complement of the β-Hex construct nucleic acids or complement of a region of the β-Hex construct nucleic acids.  
     7. Peptides  
     a) Protein Variants  
      As discussed herein there are numerous variants of the HEX-α and HEX-β proteins that are known and herein contemplated. In addition, to the known functional species and allelic variants of HEX-α and HEX-β there are derivatives of the HEX-α and HEX-β proteins which also function in the disclosed methods and compositions. Protein variants and derivatives are well understood to those of skill in the art and in can involve amino acid sequence modifications. For example, amino acid sequence modifications typically fall into one or more of three classes: substitutional, insertional or deletional variants. Insertions include amino and/or carboxyl terminal fusions as well as intrasequence insertions of single or multiple amino acid residues. Insertions ordinarily will be smaller insertions than those of amino or carboxyl terminal fusions, for example, on the order of one to four residues. Immunogenic fusion protein derivatives, such as those described in the examples, are made by fusing a polypeptide sufficiently large to confer immunogenicity to the target sequence by cross-linking in vitro or by recombinant cell culture transformed with DNA encoding the fusion. Deletions are characterized by the removal of one or more amino acid residues from the protein sequence. Typically, no more than about from 2 to 6 residues are deleted at any one site within the protein molecule. These variants ordinarily are prepared by site specific mutagenesis of nucleotides in the DNA encoding the protein, thereby producing DNA encoding the variant, and thereafter expressing the DNA in recombinant cell culture. Techniques for making substitution mutations at predetermined sites in DNA having a known sequence are well known, for example M13 primer mutagenesis and PCR mutagenesis. Amino acid substitutions are typically of single residues, but can occur at a number of different locations at once; insertions usually will be on the order of about from 1 to 10 amino acid residues; and deletions will range about from 1 to 30 residues. Deletions or insertions preferably are made in adjacent pairs, i.e. a deletion of 2 residues or insertion of 2 residues. Substitutions, deletions, insertions or any combination thereof may be combined to arrive at a final construct. The mutations must not place the sequence out of reading frame and preferably will not create complementary regions that could produce secondary mRNA structure. Substitutional variants are those in which at least one residue has been removed and a different residue inserted in its place. Such substitutions generally are made in accordance with the following Tables I and 2 and are referred to as conservative substitutions.  
               TABLE 1                          Amino Acid Abbreviations                             Amino Acid   Abbreviations                       alanine   AlaA           allosoleucine   AIle           arginine   ArgR           asparagine   AsnN           aspartic acid   AspD           cysteine   CysC           glutamic acid   GluE           glutamine   GlnK           glycine   GlyG           histidine   HisH           isolelucine   IleI           leucine   LeuL           lysine   LysK           phenylalanine   PheF           proline   ProP           pyroglutamic acidp   Glu           serine   SerS           threonine   ThrT           tyrosine   TyrY           tryptophan   TrpW           valine   ValV                      
 
     
       
         
           
               
             
               
                 TABLE 2 
               
               
                   
               
               
                   
               
               
                 Amino Acid Substitutions 
               
               
                 Original Residue Exemplary Conservative Substitutions, 
               
               
                 others are known in the art. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Ala 
                 ser 
               
               
                   
                 Arg 
                 lys, gln 
               
               
                   
                 Asn 
                 gln; his 
               
               
                   
                 Asp 
                 glu 
               
               
                   
                 Cys 
                 ser 
               
               
                   
                 Gln 
                 asn, lys 
               
               
                   
                 Glu 
                 asp 
               
               
                   
                 Gly 
                 pro 
               
               
                   
                 His 
                 asn; gln 
               
               
                   
                 Ile 
                 leu; val 
               
               
                   
                 Leu 
                 ile; val 
               
               
                   
                 Lys 
                 arg; gln; 
               
               
                   
                 Met 
                 Leu; ile 
               
               
                   
                 Phe 
                 met; leu; tyr 
               
               
                   
                 Ser 
                 thr 
               
               
                   
                 Thr 
                 ser 
               
               
                   
                 Trp 
                 tyr 
               
               
                   
                 Tyr 
                 trp; phe 
               
               
                   
                 Val 
                 ile; leu 
               
               
                   
                   
               
            
           
         
       
     
      Substantial changes in function or immunological identity are made by selecting substitutions that are less conservative than those in Table 2, i.e., selecting residues that differ more significantly in their effect on maintaining (a) the structure of the polypeptide backbone in the area of the substitution, for example as a sheet or helical conformation, (b) the charge or hydrophobicity of the molecule at the target site or (c) the bulk of the side chain. The substitutions which in general are expected to produce the greatest changes in the protein properties will be those in which (a) a hydrophilic residue, e.g. seryl or threonyl, is substituted for (or by) a hydrophobic residue, e.g. leucyl, isoleucyl, phenylalanyl, valyl or alanyl; (b) a cysteine or proline is substituted for (or by) any other residue; (c) a residue having an electropositive side chain, e.g., lysyl, arginyl, or histidyl, is substituted for (or by) an electronegative residue, e.g., glutamyl or aspartyl; or (d) a residue having a bulky side chain, e.g., phenylalanine, is substituted for (or by) one not having a side chain, e.g., glycine, in this case, (e) by increasing the number of sites for sulfation and/or glycosylation.  
      For example, the replacement of one amino acid residue with another that is biologically and/or chemically similar is known to those skilled in the art as a conservative substitution. For example, a conservative substitution would be replacing one hydrophobic residue for another, or one polar residue for another. The substitutions include combinations such as, for example, Gly, Ala; Val, Ile, Leu; Asp, Glu; Asn, Gln; Ser, Thr; Lys, Arg; and Phe, Tyr. Such conservatively substituted variations of each explicitly disclosed sequence are included within the mosaic polypeptides provided herein.  
      Substitutional or deletional mutagenesis can be employed to insert sites for N-glycosylation (Asn-X-Thr/Ser) or O-glycosylation (Ser or Thr). Deletions of cysteine or other labile residues also may be desirable. Deletions or substitutions of potential proteolysis sites, e.g. Arg, is accomplished for example by deleting one of the basic residues or substituting one by glutaminyl or histidyl residues.  
      Certain post-translational derivatizations are the result ofthe action of recombinant host cells on the expressed polypeptide. Glutaminyl and asparaginyl residues are frequently post-translationally deamidated to the corresponding glutamyl and asparyl residues. Alternatively, these residues are deamidated under mildly acidic conditions. Other post-translational modifications include hydroxylation of proline and lysine, phosphorylation of hydroxyl groups of seryl or threonyl residues, methylation of the o-amino groups of lysine, arginine, and histidine side chains (T. E. Creighton, Proteins: Structure and Molecular Properties, W. H. Freeman &amp; Co., San Francisco pp 79-86 [1983]), acetylation of the N-terminal amine and, in some instances, amidation of the C-terminal carboxyl.  
      It is understood that one way to define the variants and derivatives of the disclosed proteins herein is through defining the variants and derivatives in terms of homology/identity to specific known sequences. For example, SEQ ID NO: 1 sets forth a particular sequence of HEX-α and SEQ ID NO:3 sets forth a particular sequence of a HEX-β protein. Specifically disclosed are variants of these and other proteins herein disclosed which have at least, 70% or 75% or 80% or 85% or 90% or 95% homology to the stated sequence. Those of skill in the art readily understand how to determine the homology of two proteins. For example, the homology can be calculated after aligning the two sequences so that the homology is at its highest level.  
      Another way of calculating homology can be performed by published algorithms. Optimal alignment of sequences for comparison may be conducted by the local homology algorithm of Smith and Waterman Adv. Appl. Math. 2: 482 (1981), by the homology alignment algorithm of Needleman and Wunsch, J. MoL Biol. 48: 443 (1970), by the search for similarity method of Pearson and Lipman, Proc. Natl. Acad. Sci. U.S.A. 85: 2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by inspection.  
      The same types of homology can be obtained for nucleic acids by for example the algorithms disclosed in Zuker, M.  Science  244:48-52, 1989, Jaeger et al.  Proc. Natl. Acad. Sci. USA  86:7706-7710, 1989, Jaeger et al.  Methods Enzymol.  183:281-306, 1989 which are herein incorporated by reference for at least material related to nucleic acid alignment.  
      It is understood that the description of conservative mutations and homology can be combined together in any combination, such as embodiments that have at least 70% homology to a particular sequence wherein the variants are conservative mutations.  
      As this specification discusses various proteins and protein sequences it is understood that the nucleic acids that can encode those protein sequences are also disclosed. This would include all degenerate sequences related to a specific protein sequence, i.e. all nucleic acids having a sequence that encodes one particular protein sequence as well as all nucleic acids, including degenerate nucleic acids, encoding the disclosed variants and derivatives of the protein sequences. Thus, while each particular nucleic acid sequence may not be written out herein, it is understood that each and every sequence is in fact disclosed and described herein through the disclosed protein sequence. For example, one of the many nucleic acid sequences that can encode the protein sequence set forth in SEQ ID NO:3 is set forth in SEQ ID NO:4. Another nucleic acid sequence that encodes the same protein sequence set forth in SEQ ID NO:3 is set forth in SEQ ID NO:11. In addition, for example, a disclosed conservative derivative of SEQ ID NO:3 is shown in SEQ ID NO:12, where the valine (V) at position 21 is changed to a isoleucine (I). It is understood that for this mutation all of the nucleic acid sequences that encode this particular derivative of the SEQ ID NO:3 polypeptide are also disclosed. It is also understood that while no amino acid sequence indicates what particular DNA sequence encodes that protein within an organism, where particular variants of a disclosed protein are disclosed herein, the known nucleic acid sequence that encodes that protein in the particular organism from which that protein arises is also known and herein disclosed and described.  
     8. Pharmaceutical Carriers/Delivery of Pharamceutical Products  
      As described above, the compositions can also be administered in vivo in a pharmaceutically acceptable carrier. By “pharmaceutically acceptable” is meant a material that is not biologically or otherwise undesirable, i.e., the material may be administered to a subject, along with the nucleic acid or vector, without causing any undesirable biological effects or interacting in a deleterious manner with any of the other components of the pharmaceutical composition in which it is contained. The carrier would naturally be selected to minimize any degradation of the active ingredient and to minimize any adverse side effects in the subject, as would be well known to one of skill in the art.  
      The compositions may be administered orally, parenterally (e.g., intravenously), by intramuscular injection, by intraperitoneal injection, transdermally, extracorporeally, topically or the like, including topical intranasal administration or administration by inhalant. As used herein, “topical intranasal administration” means delivery of the compositions into the nose and nasal passages through one or both of the nares and can comprise delivery by a spraying mechanism or droplet mechanism, or through aerosolization of the nucleic acid or vector. Administration of the compositions by inhalant can be through the nose or mouth via delivery by a spraying or droplet mechanism. Delivery can also be directly to any area of the respiratory system (e.g., lungs) via intubation. The exact amount of the compositions required will vary from subject to subject, depending on the species, age, weight and general condition of the subject, the severity of the allergic disorder being treated, the particular nucleic acid or vector used, its mode of administration and the like. Thus, it is not possible to specify an exact amount for every composition. However, an appropriate amount can be determined by one of ordinary skill in the art using only routine experimentation given the teachings herein.  
      Parenteral administration of the composition, if used, is generally characterized by injection. Injectables can be prepared in conventional forms, either as liquid solutions or suspensions, solid forms suitable for solution of suspension in liquid prior to injection, or as emulsions. A more recently revised approach for parenteral administration involves use of a slow release or sustained release system such that a constant dosage is maintained. See, e.g., U.S. Pat. No. 3,610,795, which is incorporated by reference herein.  
      The materials may be in solution, suspension (for example, incorporated into microparticles, liposomes, or cells). These may be targeted to a particular cell type via antibodies, receptors, or receptor ligands. The following references are examples of the use of this technology to target specific proteins to tumor tissue (Senter, et al.,  Bioconjugate Chem.,  2:447-451, (1991); Bagshawe, K. D.,  Br. J. Cancer,  60:275-281, (1989); Bagshawe, et al.,  Br. J. Cancer,  58:700-703, (1988); Senter, et al.,  Bioconjugate Chem.,  4:3-9, (1993); Battelli, et al.,  Cancer Immunol. Inmunother.,  35:421-425, (1992); Pietersz and McKenzie,  Immunolog. Reviews,  129:57-80, (1992); and Roffler, et al.,  Biochem. Pharmacol,  42:2062-2065, (1991)). Vehicles such as “stealth” and other antibody conjugated liposomes (including lipid mediated drug targeting to colonic carcinoma), receptor mediated targeting of DNA through cell specific ligands, lymphocyte directed tumor targeting, and highly specific therapeutic retroviral targeting of murine glioma cells in vivo. The following references are examples of the use of this technology to target specific proteins to tumor tissue (Hughes et al.,  Cancer Research,  49:6214-6220, (1989); and Litzinger and Huang,  Biochimica et Biophysica Acta,  1104:179-187, (1992)). In general, receptors are involved in pathways of endocytosis, either constitutive or ligand induced. These receptors cluster in clathrin-coated pits, enter the cell via clathrin-coated vesicles, pass through an acidified endosome in which the receptors are sorted, and then either recycle to the cell surface, become stored intracellularly, or are degraded in lysosomes. The internalization pathways serve a variety of functions, such as nutrient uptake, removal of activated proteins, clearance of macromolecules, opportunistic entry of viruses and toxins, dissociation and degradation of ligand, and receptor-level regulation. Many receptors follow more than one intracellular pathway, depending on the cell type, receptor concentration, type of ligand, ligand valency, and ligand concentration. Molecular and cellular mechanisms of receptor-mediated endocytosis has been reviewed (Brown and Greene,  DNA and Cell Biology  10:6, 399-409 (1991)).  
     a) Pharmaceutically Acceptable Carriers  
      The compositions, including antibodies, can be used therapeutically in combination with a pharmaceutically acceptable carrier.  
      Suitable carriers and their formulations are described in  Remington: The Science and Practice of Pharmacy  (19th ed.) ed. A. R. Gennaro, Mack Publishing Company, Easton, Pa. 1995. Typically, an appropriate amount of a pharmaceutically-acceptable salt is used in the formulation to render the formulation isotonic. Examples of the pharmaceutically-acceptable carrier include, but are not limited to, saline, Ringer&#39;s solution and dextrose solution. The pH of the solution is preferably from about 5 to about 8, and more preferably from about 7 to about 7.5. Further carriers include sustained release preparations such as semipermeable matrices of solid hydrophobic polymers containing the antibody, which matrices are in the form of shaped articles, e.g., films, liposomes or microparticles. It will be apparent to those persons skilled in the art that certain carriers may be more preferable depending upon, for instance, the route of administration and concentration of composition being administered.  
      Pharmaceutical carriers are known to those skilled in the art. These most typically would be standard carriers for administration of drugs to humans, including solutions such as sterile water, saline, and buffered solutions at physiological pH. The compositions can be administered intramuscularly or subcutaneously. Other compounds will be administered according to standard procedures used by those skilled in the art.  
      Pharmaceutical compositions may include carriers, thickeners, diluents, buffers, preservatives, surface active agents and the like in addition to the molecule of choice. Pharmaceutical compositions may also include one or more active ingredients such as antimicrobial agents, antiinflammatory agents, anesthetics, and the like.  
      The pharmaceutical composition may be administered in a number of ways depending on whether local or systemic treatment is desired, and on the area to be treated. Administration may be topically (including ophthalmically, vaginally, rectally, intranasally), orally, by inhalation, or parenterally, for example by intravenous drip, subcutaneous, intraperitoneal or intramuscular injection. The disclosed antibodies can be administered intravenously, intraperitoneally, intramuscularly, subcutaneously, intracavity, or transdermally.  
      Preparations for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of non-aqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media. Parenteral vehicles include sodium chloride solution, Ringer&#39;s dextrose, dextrose and sodium chloride, lactated Ringer&#39;s, or fixed oils. Intravenous vehicles include fluid and nutrient replenishers, electrolyte replenishers (such as those based on Ringer&#39;s dextrose), and the like. Preservatives and other additives may also be present such as, for example, antimicrobials, anti-oxidants, chelating agents, and inert gases and the like.  
      Formulations for topical administration may include ointments, lotions, creams, gels, drops, suppositories, sprays, liquids and powders. Conventional pharmaceutical carriers, aqueous, powder or oily bases, thickeners and the like may be necessary or desirable.  
      Compositions for oral administration include powders or granules, suspensions or solutions in water or non-aqueous media, capsules, sachets, or tablets. Thickeners, flavorings, diluents, emulsifiers, dispersing aids or binders may be desirable.  
      Some of the compositions may potentially be administered as a pharmaceutically acceptable acid- or base- addition salt, formed by reaction with inorganic acids such as hydrochloric acid, hydrobromic acid, perchloric acid, nitric acid, thiocyanic acid, sulfuric acid, and phosphoric acid, and organic acids such as formic acid, acetic acid, propionic acid, glycolic acid, lactic acid, pyruvic acid, oxalic acid, malonic acid, succinic acid, maleic acid, and fumaric acid, or by reaction with an inorganic base such as sodium hydroxide, ammonium hydroxide, potassium hydroxide, and organic bases such as mono-, di-, trialkyl and aryl amines and substituted ethanolamines.  
     9. Chips and Micro Arrays  
      Disclosed are chips where at least one address is the sequences or part of the sequences set forth in any of the nucleic acid sequences disclosed herein. Also disclosed are chips where at least one address is the sequences or portion of sequences set forth in any of the peptide sequences disclosed herein.  
      Also disclosed are chips where at least one address is a variant of the sequences or part of the sequences set forth in any of the nucleic acid sequences disclosed herein. Also disclosed are chips where at least one address is a variant of the sequences or portion of sequences set forth in any of the peptide sequences disclosed herein.  
     10. Computer Readable Mediums  
      It is understood that the disclosed nucleic acids and proteins can be represented as a sequence consisting of the nucleotides of amino acids. There are a variety of ways to display these sequences, for example the nucleotide guanosine can be represented by G or g. Likewise the amino acid valine can be represented by Val or V. Those of skill in the art understand how to display and express any nucleic acid or protein sequence in any of the variety of ways that exist, each of which is considered herein disclosed. Specifically contemplated herein is the display of these sequences on computer readable mediums, such as, commercially available floppy disks, tapes, chips, hard drives, compact disks, and video disks, or other computer readable mediums. Also disclosed are the binary code representations of the disclosed sequences. Those of skill in the art understand what computer readable mediums. Thus, computer readable mediums on which the nucleic acids or protein sequences are recorded, stored, or saved.  
      Disclosed are computer readable mediums comprising the sequences and information regarding the sequences set forth herein.  
     11. Kits  
      Disclosed herein are kits that are drawn to reagents that can be used in practicing the methods disclosed herein. The kits can include any reagent or combination of reagent discussed herein or that would be understood to be required or beneficial in the practice of the disclosed methods. For example, the kits could include primers to perform the amplification reactions discussed in certain embodiments of the methods, as well as the buffers and enzymes required to use the primers as intended.  
     D. Methods of Making the Compositions  
      The compositions disclosed herein and the compositions necessary to perform the disclosed methods can be made using any method known to those of skill in the art for that particular reagent or compound unless otherwise specifically noted.  
      The disclosed viral vectors can be made using standard recombinant molecular biology techniques. Many of these techniques are illustrated in Maniatis (Maniatis et al., “ Molecular Cloning—A Laboratory Manual, ” (Cold Spring Harbor Laboratory, Latest edition) and Sambrook et al.,  Molecular Cloning: A Laboratory Manual,  2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989.  
     1. Nucleic Acid Synthesis  
      For example, the nucleic acids, such as, the oligonucleotides to be used as primers can be made using standard chemical synthesis methods or can be produced using enzymatic methods or any other known method. Such methods can range from standard enzymatic digestion followed by nucleotide fragment isolation (see for example, Sambrook et al.,  Molecular Cloning: A Laboratory Manual,  2nd Edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989) Chapters 5, 6) to purely synthetic methods, for example, by the cyanoethyl phosphoramidite method using a Milligen or Beckman System Plus DNA synthesizer (for example, Model 8700 automated synthesizer of Milligen-Biosearch, Burlington, MA or ABI Model 380B). Synthetic methods useful for making oligonucleotides are also described by Ikuta et al.,  Ann. Rev. Biochem.  53:323-356 (1984), (phosphotriester and phosphite-triester methods), and Narang et al.,  Methods Enzymol.,  65:610-620 (1980), (phosphotriester method). Protein nucleic acid molecules can be made using known methods such as those described by Nielsen et al.,  Bioconjug. Chem.  5:3-7 (1994).  
     2. Peptide Synthesis  
      One method of producing the disclosed proteins is to link two or more peptides or polypeptides together by protein chemistry techniques. For example, peptides or polypeptides can be chemically synthesized using currently available laboratory equipment using either Fmoc (9-fluorenylmethyloxycarbonyl) or Boc (tert -butyloxycarbonoyl) chemistry. (Applied Biosystems, Inc., Foster City, Calif.). One skilled in the art can readily appreciate that a peptide or polypeptide corresponding to the disclosed proteins, for example, can be synthesized by standard chemical reactions. For example, a peptide or polypeptide can be synthesized and not cleaved from its synthesis resin whereas the other fragment of a peptide or protein can be synthesized and subsequently cleaved from the resin, thereby exposing a terminal group which is functionally blocked on the other fragment. By peptide condensation reactions, these two fragments can be covalently joined via a peptide bond at their carboxyl and amino termini, respectively, to form an antibody, or fragment thereof. (Grant G A (1992) Synthetic Peptides: A User Guide. W.H. Freeman and Co., N.Y. (1992); Bodansky M and Trost B., Ed. (1993) Principles of Peptide Synthesis. Springer-Verlag Inc., NY (which is herein incorporated by reference at least for material related to peptide synthesis). Alternatively, the peptide or polypeptide is independently synthesized in vivo as described herein. Once isolated, these independent peptides or polypeptides may be linked to form a peptide or fragment thereof via similar peptide condensation reactions.  
      For example, enzymatic ligation of cloned or synthetic peptide segments allow relatively short peptide fragments to be joined to produce larger peptide fragments, polypeptides or whole protein domains (Abrahmsen L et al., Biochemistry, 30:4151 (1991)). Alternatively, native chemical ligation of synthetic peptides can be utilized to synthetically construct large peptides or polypeptides from shorter peptide fragments. This method consists of a two step chemical reaction (Dawson et al. Synthesis of Proteins by Native Chemical Ligation. Science, 266:776-779 (1994)). The first step is the chemoselective reaction of an unprotected synthetic peptide—thioester with another unprotected peptide segment containing an amino-terminal Cys residue to give a thioester-linked intermediate as the initial covalent product. Without a change in the reaction conditions, this intermediate undergoes spontaneous, rapid intramolecular reaction to form a native peptide bond at the ligation site (Baggiolini M et al. (1992) FEBS Lett. 307:97-101; Clark-Lewis I et al., J.Biol.Chem., 269:16075 (1994); Clark-Lewis I et al., Biochemistry, 30:3128 (1991); Rajarathnam K et al., Biochemistry 33:6623-30 (1994)).  
      Alternatively, unprotected peptide segments are chemically linked where the bond formed between the peptide segments as a result of the chemical ligation is an unnatural (non-peptide) bond (Schnolzer, M et al. Science, 256:221 (1992)). This technique has been used to synthesize analogs of protein domains as well as large amounts of relatively pure proteins with full biological activity (deLisle Milton R C et al., Techniques in Protein Chemistry IV. Academic Press, New York, pp. 257-267 (1992)).  
     3. Processes for Making the Compositions  
      Disclosed are processes for making the compositions as well as making the intermediates leading to the compositions. There are a variety of methods that can be used for making these compositions, such as synthetic chemical methods and standard molecular biology methods. It is understood that the methods of making these and the other disclosed compositions are specifically disclosed.  
      Disclosed are nucleic acid molecules produced by the process comprising linking in an operative way a promoter element, a HexB element, a IRES element, and a HexA element.  
      Disclosed are nucleic acid molecules produced by the process comprising linking in an operative way nucleic acid molecules comprising sequences set forth in SEQ ID NO:10 and SEQ ID NO:4.  
      Also disclosed are nucleic acid molecules produced by the process comprising linking in an operative way nucleic acid molecules comprising sequences having 80% identity to sequences set forth in SEQ ID NO:10 and SEQ ID NO:4.  
      Also disclosed are nucleic acid molecules produced by the process comprising linking in an operative way nucleic acid molecules comprising sequences that hybridizes under stringent hybridization conditions to sequences set forth in SEQ ID NO:10 and SEQ ID NO:4.  
      Disclosed are nucleic acid molecules produced by the process comprising linking in an operative way a nucleic acid molecule comprising a sequence encoding HEX-β and HEX-α peptides and a sequence controlling an expression of the sequence encoding HEX-β and HEX-α.  
      Disclosed are nucleic acid molecules produced by the process comprising linking in an operative way a nucleic acid molecule comprising a sequence encoding HEX-β and HEX-α peptides wherein the HEX-β and HEX-α peptides have 80% identity to the peptides set forth in SEQ ID NO:1 and SEQ ID NO:3 and a sequence controlling expression of the sequences encoding the peptides.  
      Disclosed are nucleic acid molecules produced by the process comprising linking in an operative way a nucleic acid molecule comprising a sequence encoding HEX-β and HEX-α peptides wherein the HEX-β and HEX-α peptides have 80% identity to the peptides set forth in SEQ ID NO:1 and SEQ ID NO:3, wherein any change from the sequences set forth in SEQ ID NO:1 and SEQ ID NO:3 are conservative changes and a sequence controlling expression of the sequences encoding the peptides.  
      Disclosed are cells produced by the process of transforming the cell with any of the disclosed nucleic acids. Disclosed are cells produced by the process of transforming the cell with any of the non-naturally occurring disclosed nucleic acids.  
      Disclosed are any of the disclosed peptides produced by the process of expressing any of the disclosed nucleic acids. Disclosed are any of the non-naturally occurring disclosed peptides produced by the process of expressing any of the disclosed nucleic acids. Disclosed are any of the disclosed peptides produced by the process of expressing any of the non-naturally disclosed nucleic acids.  
      Disclosed are animals produced by the process of transfecting a cell within the animal with any of the nucleic acid molecules disclosed herein. Disclosed are animals produced by the process of transfecting a cell within the animal any of the nucleic acid molecules disclosed herein, wherein the animal is a mammal. Also disclosed are animals produced by the process of transfecting a cell within the animal any of the nucleic acid molecules disclosed herein, wherein the mammal is mouse, rat, rabbit, cow, sheep, pig, or primate. Also disclosed are mammals wherein mammal is a murine, ungulate, or non-human primate.  
      Also disclose are animals produced by the process of adding to the animal any of the cells disclosed herein.  
     E. Methods of Using the Compositions  
     1. Methods of Using the Compositions as Research Tools  
      The disclosed compositions can be used in a variety of ways as research tools. For example, the disclosed compositions, the B-Hex constructs, and other nucleic acids, such as SEQ ID NOs:10 and 4 can be used to produce organisms, such as transgenic or knockout mice, which can be used as model systems for the study of Tay Sachs and Sandoffs disease.  
     2. Methods of Gene Modification and Gene Disruption  
      The disclosed compositions and methods can be used for targeted gene disruption and modification in any animal that can undergo these events. Gene modification and gene disruption refer to the methods, techniques, and compositions that surround the selective removal or alteration of a gene or stretch of chromosome in an animal, such as a mammal, in a way that propagates the modification through the germ line of the mammal. In general, a cell is transformed with a vector which is designed to homologously recombine with a region of a particular chromosome contained within the cell, as for example, described herein. This homologous recombination event can produce a chromosome which has exogenous DNA introduced, for example in frame, with the surrounding DNA. This type of protocol allows for very specific mutations, such as point mutations, to be introduced into the genome contained within the cell. Methods for performing this type of homologous recombination are disclosed herein.  
      One of the preferred characteristics of performing homologous recombination in mammalian cells is that the cells should be able to be cultured, because the desired recombination event occurs at a low frequency.  
      Once the cell is produced through the methods described herein, an animal can be produced from this cell through either stem cell technology or cloning technology. For example, if the cell into which the nucleic acid was transfected was a stem cell for the organism, then this cell, after transfection and culturing, can be used to produce an organism which will contain the gene modification or disruption in germ line cells, which can then in turn be used to produce another animal that possesses the gene modification or disruption in all of its cells. In other methods for production of an animal containing the gene modification or disruption in all of its cells, cloning technologies can be used. These technologies generally take the nucleus of the transfected cell and either through fusion or replacement fuse the transfected nucleus with an oocyte which can then be manipulated to produce an animal. The advantage of procedures that use cloning instead of ES technology is that cells other than ES cells can be transfected. For example, a fibroblast cell, which is very easy to culture can be used as the cell which is transfected and has a gene modification or disruption event take place, and then cells derived from this cell can be used to clone a whole animal.  
     3. Therapeutic Uses  
      Effective dosages and schedules for administering the compositions may be determined empirically, and making such determinations is within the skill in the art. The dosage ranges for the administration of the compositions are those large enough to produce the desired effect in which the symptoms disorder are effected. The dosage should not be so large as to cause adverse side effects, such as unwanted cross-reactions, anaphylactic reactions, and the like. Generally, the dosage will vary with the age, condition, sex and extent of the disease in the patient, route of administration, or whether other drugs are included in the regimen, and can be determined by one of skill in the art. The dosage can be adjusted by the individual physician in the event of any counterindications. Dosage can vary, and can be administered in one or more dose administrations daily, for one or several days. Guidance can be found in the literature for appropriate dosages for given classes of pharmaceutical products.  
      Following administration of a disclosed composition, such as the disclosed constructs, for treating, inhibiting, or preventing Tay Sachs or Sandoffs disease, the efficacy of the therapeutic construct can be assessed in various ways well known to the skilled practitioner. For instance, one of ordinary skill in the art will understand that a composition, such as the disclosed constructs, disclosed herein is efficacious in treating Tay Sachs or Sandoffs disease or inhibiting or reducing the effects of Tay Sachs or Sandoffs disease in a subject by observing that the composition reduces the onset of the conditions associated with these diseases. Furthermore, the amount of protein or transcript produced from the constructs can be analyzed using any diagnostic method. For example, it can be measured using polymerase chain reaction assays to detect the presence of construct nucleic acid or antibody assays to detect the presence of protein produced from the construct in a sample (e.g., but not limited to, blood or other cells, such as neural cells) from a subject or patient.  
     F. EXAMPLES  
      It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope or spirit of the invention. Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the invention being indicated by the following claims.  
      The following examples are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how the compounds, compositions, articles, devices and/or methods claimed herein are made and evaluated, and are intended to be purely exemplary of the invention and are not intended to limit the scope of what the inventors regard as their invention. Efforts have been made to ensure accuracy with respect to numbers (e.g., amounts, temperature, etc.), but some errors and deviations should be accounted for. Unless indicated otherwise, parts are parts by weight, temperature is in ° C or is at ambient temperature, and pressure is at or near atmospheric.  
     1. Example 1  
     Making β-Hex Constructs  
     a) Construction of Bicistronic β-Hex Construct  
      A bicistronic construct encoding for both isoforms of human β-hexosaminidase, hHexA and hHexB was made ( FIG. 1 ). hHexB cDNA was isolated following Xho I digestion of pHexB43 (ATCC, Manassas Va.) and cloned into the Xho I site of pIRES (Clonetech Laboratories, Palo Alto Calif.) downstream of the vector&#39;s cytomegalovirus (CMV) promoter sequence. The HexA cDNA was isolated from pBHA-5 (ATCC, Manassas Va.) by Xho I digestion and was subsequently inserted into the Xba I site of pIRES(HexB) downstream of the vectors IRES cassette by blunt ligation. In this construct, the cytomegalovirus promoter (CMV) drives transgene expression, and the translation of the second open reading frame, HexB, is facilitated by an internal ribosomal entry sequence (IRES).  
     b) Results  
      The HEXlacZ encodes for both isoforms of human β-hexosaminidase, HexA &amp; HexB. ( FIG. 1 ) The vector pHEXlacZ is shown in  FIG. 1 (A). BHK HeXlacZ  are developed by stable HexlacZ transduction.  FIG. 1 (B) shows that the cells transfected with the pHEXlacZ vector stain positively by X-gal histochemistry. Furthermore, HexA &amp; HexB mRNA was detected by RT-PCR in total RNA extracts ( FIG. 1 (C)). Likewise, not only was transcript of pHEXlacZ vector identified, human HEXA and human HEXB proteins were detected in the transfected BHK HexlacZ  cells by imunocytochemistry. ( FIG. 1 (D 1 ) and  1 (E 1 ). This data indicates that the disclosed constructs can be expressed in target cells and that sufficient levels of protein are produced within these cells.  
      The β-Hex therapeutic gene is capable of correcting deficiencies in cells that are not transfected through cross-correction. ( FIG. 2 ) An important property of the β-Hex transgene is the products hHEXA &amp; hHEXB have the ability to cross-correct, specifically, to be released extracellularly and then to be absorbed via paracrine pathways by other cells whereby they contribute to β-hexosaminidase activity. BHK HexlacZ  cells were cultured and the supernatant was collected (conditioned medium), filtered (0.45 mm) and applied on normal mouse kidney fibroblasts in culture. Forty-eight hours later, the cells were washed thoroughly with phosphate buffered saline, and briefly treated with a trypsin solution to remove extracellular proteins from the cell surfaces. Following trypsin inactivation with Tris/EDTA buffer, the cells were fixed with 4% paraformaldehyde solution and processed by Fast Garnet histochemistry for β-hexosaminidase activity. Fast Garnet histochemistry of murine fibroblasts exposed to ( FIG. 2A ) conditioned medium collected from BHK HexlacZ  cells compared to cells exposed to medium from normal parent BHK-21 cells ( FIG. 2B ). These results demonstrate that hHEXA &amp; hHEXB, products of the β-Hex transgene, are released into the extracellular medium and can be absorbed by other cells via paracrine pathways resulting in induction of the cellular β-hexosaminidase.  
     2. Example 2  
     Transfecting Constructs  
     a) Construction of the Tricistronic β-Hex Construct  
      A tricistronic construct encoding for both isoforms of human β-hexosaminidase, hHexA &amp; hHexB, as well as the β-galactosidase reporter gene (lacZ) was also made. hHexB cDNA was isolated following Xho I digestion of pHexB43 (ATCC, Manassas Va.) and cloned into the Xho I site of pIRES (Clonetech Laboratories, Palo Alto Calif.) downstream of the vector&#39;s cytomegalovirus (CMV) promoter sequence. The HexA cDNA was isolated from pBHA-5 (ATCC, Manassas Va.) by Xho I digestion and was subsequently inserted into the Xba I site of pIRES(HexB) downstream of the vector&#39;s IRES cassette by blunt ligation. A IRES-lacZ cassette was obtained from Dr. Howard J. Federoff, University of Rochester School of Medicine and Dentistry, but can be produced using standard recombinant techniques with known reagents and was inserted downstream to HexA into the Sal I site of pHexB-IRES-HexA by blunt ligation. In this construct, the cytomegalovirus promoter (CMV) drives transgene expression, and the translation of the second and third open reading frames (ORF), HexB and lacZ, respectively, are facilitated by an internal ribosomal entry sequence (IRES). The FIV(Hex) vector was constructed by isolating the HexB-IRES-HexA (β-Hex) fragment of pHexlacZ with NheI-NotI digestion is present and it was cloneed into the FIV backbone (Poeschla et al., 1998), derived after excising the lacZ cassette from pFIV(lacZ) with Bpu1102I, leading to the successful construction of pFIV(Hex) (See  FIGS. 3 and 4 ). Restriction fragment analysis indicated that pFIV(Hex) was constructed as designed. ( FIG. 5 ).  
      The viral derived IRES sequence can effectively drive the expression of second genes in bicistronic constructs in vitro and in vivo, (Gurtu et al., 1996; Geschwind et al., 1996; Havenga et al. 1998). Nevertheless, IRES-mediated transcription in bicistronic constructs has been shown to reduce the levels of expression of the second ORF by about 40-50%. Hence, since HexB is necessary in the synthesis of both HEXA (α/β) and HEXB (α/α), it was cloned first in our tricistronic construct. Confirmation of the construct has been achieved by multiple restriction enzyme digestions as well as direct DNA sequencing.  
     b) Results  
      The FIV backbone vector was isolated from the FIV(lacZ) vector following Sst II &amp; Not I digestion. The bicistronic transgene HexB-IRES-HexA was extracted from the pHexlacZ vector following Nhe I &amp; Not I digestion, and was cloned into the FIV backbone by blunt ligation. FIV(Hex) digestion with the restriction enzymes Xho I and Sal I confirmed the cloning. ( FIG. 6 ) FIV(Hex) virus was prepared using established methods and was tested in vitro as follows. Cultured murine fibroblasts (CrfK cell line) were exposed to FIV(Hex) for 12 hours, followed fresh media change. After 48 hours, cellular DNA and RNA extracts were collected. The presence of viral DNA was assessed by PCR with primers sets specifically designed for HexB ( FIG. 6A ). HexB expression was assessed by RT-PCR ( FIG. 6B ). These results demonstrate the ability of FIV(Hex) to transduce mouse fibroblasts with β-Hex, resulting in transgene mRNA expression. ( FIG. 6 ).  
      The tricistronic vector pHEXlacZ was stably expressed in embryonic hamster kidney fibroblasts (BHK-21; ATCC) following standard transfection laboratory techniques using the LIPOFECTAMINE® reagent (Gibco BRL) per manufacturer&#39;s instructions. Forty-eight hours post-transfection, the cells were treated with 800 μg/mL G418 (Gibco BRL) for 10 days, and cell lines were selected, expanded and analyzed for expression of our tricistronic gene as follows. Analysis of the transfected cells showed that cell lines (Crfk, spleen, brain, liver, and kidney) stained positively for X-gal, indicating expression of and translation of the expressed product from the tricistronic vector. ( FIG. 6 )  
     3. Example 4  
     In Vivo Use of FIV HEX Vectors  
      FIV(Hex) was constructed by inserting the bicistronic gene HexB-IRES-HexA in the place of the reporter gene lacZ in the FIV backbone vector using standard mmolecular biology techniques. FIV(Hex) was prepared in vitro by transient co-transfection of the transfer vector along with the packaging and envelop plasmids into 293H cells. The virus-rich supernatant was centrifuged and the viral pellet was reconstituted in normal saline, and was then titered in CrfK cells by the X-Hex histochemical method (10 7 -10 8  infectious particles/ml). The viral solution was injected intraperitoneally to 2 days old HexB −/−  knockout mouse pups, which were allowed to reach the critical age of 16 weeks, when they displayed full signs of the lysosomal storage disease. For control, litermates were injected with the FIV(lacZ) virus, which is identical to FIV(Hex), but instead of carrying the HexB-IRES-HexA gene it carries the reporter gene lacZ. Locomotive performance was evaluated by placing the mice on a wire mesh attached on a clear plexiglass cylinder, and turning the wire mesh up-side-down. The lapse time until the mice fell off the wire mesh was recorded on weekly basis until the mice were terminated. It is important to state that at the critical time point of 16 weeks, the FIV(Hex) injected mice showed statistically better locomotive performance compared to FIV(lacZ) injected mice (controls). Furthermore, the FIV(Hex) mice had an extended life span for at least 2-3 additional weeks, at which point they were also terminated because they were showing signs of the disease.  
     4. Example 3  
     HIV HEX Vectors  
      The HexB-IRES-HexA therapeutic gene was cloned into the Lenti6/V5D-TOPO vector commercially available by Invitrogen (Carlsbad, Calif.), whereby the cytomegalovirus promoter CMV drives gene expression [in a manner similar to FIV(Hex)]. A virus was constructed whereby the expression of HexB-IRES-HexA is driven by a promoter, such as that show in SEQ ID NO:23, which consists of a beta-actin portion and a CMV portion. This type of promoter has high expression in mammalian cells.  
       
      G. REFERENCES  
      Akli S, Guidotti J-E, Vigne E, Perricaudet M, Sandhoff K, Kahn A, Poenaru L (1996) Restoration of hexosaminidase A activity in human Tay-Sachs fibriblasts via adenoviral vector mediated gene transfer. Gene Therapy 3: 769-774  
      Bowers W J, Howard D F, Federoff H J (2000). Discordance between expression and genome transfer titering of HSV amplicon vectors: recommendation for standardized enumeration. Mol Ther 1:294-9.  
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     H. Sequences  
      1. SEQ ID NO:1  Homo sapiens  hexosaminidase A (alpha polypeptide) (HEXA), Genbank Accession No. XM — 037778  
      2. SEQ ID NO:2  Homo sapiens  hexosaminidase A (alpha polypeptide) (HEXA), Genbank Accession No. XM — 037778  
      3. SEQ ID NO:3  Homo sapiens  hexosaminidase B (beta polypeptide) (HEXB), protein Genbank Accession No XM — 032554  
      4. SEQ ID NO:4  Homo sapiens  hexosaminidase B (beta polypeptide) (HEXB), mRNA Genbank Accession No XM — 032554  
      5. SEQ ID NO:5 IRES sequence U.S. Pat. No.4,937,190 herein incorporated by reference covers entire Vector, and is cited at least for material relating to the pIRES vector)  
      6. SEQ ID NO:6  Mus musculus  hexosaminidase A (Hexa), protein Genbank Accession No, NM — 010421  
      7. SEQ ID NO:7  Mus musculus  hexosaminidase A (Hexa), mRNA Genbank Accession No, NM — 010421  
      8. SEQ ID NO:8 FIV(LacZ) construct 12750 bp  
      9. SEQ ID NO:9: HEX-α polypeptide Genbank accession number NM — 000520 (Proia) beta-hexosaminidase A alpha-subunit to human chromosomal region 15q23—q24  
      10. SEQ ID NO:10 HexA gene Genbank accession number NM — 000520 (Proia)  
      11. SEQ ID NO:11 HexB degenerate cDNA G to A change at position 6  
      12. SEQ ID NO:12: HEX-β polypeptide conservative substitution of Val21 to I21  
      13. SEQ ID NO:13 HEX-α polypeptide Genbank accession number M16411 (Tissue sample from ATCC)  
      14. SEQ ID NO:14 HexA gene Genbank accession number M16411  
      15. SEQ ID NO:15: HEX-β polypeptide Genbank accession number NM — 000521 (Proia) beta-hexosaminidase A alpha-subunit to human chromosomal region chromosome 5 map=“5q13” 
      16. SEQ ID NO:16 HexB gene Genbank accession number NM — 000521 Proia  
      17. SEQ ID NO:17  Mus musculus  hexosaminidase B (Hexb), protein. Genbank Accession No. NM — 010422  
      18. SEQ ID NO:18  Mus musculus  hexosaminidase B (Hexb), mRNA. Genbank Accession No. NM — 010422  
      19. SEQ ID NO:19 Bactin Hex sequence  
      20. SEQ ID NO:20 HIV Hex vector sequence  
      21. SEQ ID NO:21 E02199 DNA encoding chicken beta actin gene promoter.  
      22. SEQ ID NO:22 Chicken Beta Actin promoter  
      23. SEQ ID NO:23 CMV-Beta actin promoter  
      24. SEQ ID NO:24 Fusion promoter-CMV portion  
      25. SEQ ID NO:25 Fusion promoter—beta actin portion  
      26. SEQ ID NO:26 Chicken beta actin promoter  
      27. SEQ ID NO:27 Accession # BD136067. promoter element for sustained gene expression from CMV promoter.  
      28. SEQ ID NO:28 BD136066 Accession # promoter element for sustained gene expression from CMV promoter.  
      29. SQ ID NO:29 BD136065 Accession # promoter element for sustained gene expression from CMV promoter.  
      30. SEQ ID NO:30 BD136064 Accession # promoter element for sustained gene expression from CMV promoter  
      31. SED ID NO:31 L77202 Accession # Murine Cytomegalovirus early (E1) gene, promoter region.  
      32. SEQ ID NO:32 X03922 Accession # Human cytomegalovirus (HCMV) IE1 gene promoter region.  
      33. SEQ ID NO:33 E06566 Accession # Promoter gene of human beta-actin gene.  
      34. SEQ ID NO:34 E02198 Accession # Dna encoding 3′ end region of beta-actin gene promoter.  
      35. SEQ ID NO:35 E02197 Accession # DNA encoding 3′ end region of beta-actin gene promoter.  
      36. SEQ ID NO:36 E02196 Accession # DNA encoding 3′ end region of beta-actin gene promoter.  
      37. SEQID NO:37 E02195 Accession # DNA encoding 3′ end region of beta-actin gene promoter.  
      38. EQ ID NO:38 E02194 Accession # DNA encoding chicken beta-actin gene promoter.  
      39. SEQ ID NO:39 E01452 Accession # Genomic DNA of promoter of human beta-actin.  
      40. SEQ ID NO E03011 Accession # DNA encoding hybrid promoter that is composed of chicken beta-actin gene promoter and rabbit beta-globin gene promoter.  
      41. SEQ ID NO:41 BD015377 Accession# Baculovirus containing minimum CMV promoter.  
      42. Other cytomegalovirus promoter regions  
      Other human cytomegalovirus promoter regions can be found in accession numbers M64940, Human cytomegalovirus IE-1 promoter region, M64944 Human cytomegalovirus IE-1 promoter region, M64943 Human cytomegalovirus IE-1 promoter region, M64942 Human cytomegalovirus IE-1 promoter region, M64941 Human cytomegalovirus IE-1 promoter region (All of which are herein incorporated by reference at least for their sequence and information)