Patent Publication Number: US-8524872-B2

Title: Nucleic acids and corresponding proteins entitled 158P3D2 useful in treatment and detection of cancer

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a divisional of U.S. patent application Ser. No. 12/899,433, filed Oct. 6, 2010, now U.S. Pat. No. 8,212,017, which is a continuation of U.S. patent application Ser. No. 10/994,106, filed Nov. 19, 2004, now U.S. Pat. No. 7,811,575, which is a continuation-in-part of U.S. patent application Ser. No. 10/107,532, filed Mar. 25, 2002, now abandoned, which claims priority to U.S. Provisional Patent Application No. 60/283,112, filed Apr. 10, 2001, and U.S. Provisional Patent Application No. 60/286,630, filed Apr. 25, 2001. The contents of the applications listed in this paragraph are fully incorporated by reference herein. 
    
    
     STATEMENT OF RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH 
     Not applicable. 
     REFERENCE TO SEQUENCE LISTING SUBMITTED VIA EFS-WEB 
     The entire content of the following electronic submission of the sequence listing via the USPTO EFS-WEB server, as authorized and set forth in MPEP §1730 II.B.2(a)(A), is incorporated herein by reference in its entirety for all purposes. The sequence listing is identified on the electronically filed text file as follows: 
     
       
         
           
               
               
               
             
               
                   
               
               
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                 511582006410Seqlist.txt 
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     FIELD OF THE INVENTION 
     The invention described herein relates to genes and their encoded proteins, termed 158P3D2 and variants thereof, expressed in certain cancers, and to diagnostic and therapeutic methods and compositions useful in the management of cancers that express 158P3D2. 
     BACKGROUND OF THE INVENTION 
     Cancer is the second leading cause of human death next to coronary disease. Worldwide, millions of people die from cancer every year. In the United States alone, as reported by the American Cancer Society, cancer causes the death of well over a half-million people annually, with over 1.2 million new cases diagnosed per year. While deaths from heart disease have been declining significantly, those resulting from cancer generally are on the rise. In the early part of the next century, cancer is predicted to become the leading cause of death. 
     Worldwide, several cancers stand out as the leading killers. In particular, carcinomas of the lung, prostate, breast, colon, pancreas, and ovary represent the primary causes of cancer death. These and virtually all other carcinomas share a common lethal feature. With very few exceptions, metastatic disease from a carcinoma is fatal. Moreover, even for those cancer patients who initially survive their primary cancers, common experience has shown that their lives are dramatically altered. Many cancer patients experience strong anxieties driven by the awareness of the potential for recurrence or treatment failure. Many cancer patients experience physical debilitations following treatment. Furthermore, many cancer patients experience a recurrence. 
     Worldwide, prostate cancer is the fourth most prevalent cancer in men. In North America and Northern Europe, it is by far the most common cancer in males and is the second leading cause of cancer death in men. In the United States alone, well over 30,000 men die annually of this disease—second only to lung cancer. Despite the magnitude of these figures, there is still no effective treatment for metastatic prostate cancer. Surgical prostatectomy, radiation therapy, hormone ablation therapy, surgical castration and chemotherapy continue to be the main treatment modalities. Unfortunately, these treatments are ineffective for many and are often associated with undesirable consequences. 
     On the diagnostic front, the lack of a prostate tumor marker that can accurately detect early-stage, localized tumors remains a significant limitation in the diagnosis and management of this disease. Although the serum prostate specific antigen (PSA) assay has been a very useful tool, however its specificity and general utility is widely regarded as lacking in several important respects. 
     Progress in identifying additional specific markers for prostate cancer has been improved by the generation of prostate cancer xenografts that can recapitulate different stages of the disease in mice. The LAPC (Los Angeles Prostate Cancer) xenografts are prostate cancer xenografts that have survived passage in severe combined immune deficient (SCID) mice and have exhibited the capacity to mimic the transition from androgen dependence to androgen independence (Klein et al., 1997, Nat. Med. 3:402). More recently identified prostate cancer markers include PCTA-1 (Su et al., 1996, Proc. Natl. Acad. Sci. USA 93: 7252), prostate-specific membrane (PSM) antigen (Pinto et al., Clin Cancer Res 1996 Sep. 2 (9): 1445-51), STEAP (Hubert, et al., Proc Natl Acad Sci USA. 1999 Dec. 7; 96(25): 14523-8) and prostate stem cell antigen (PSCA) (Reiter et al., 1998, Proc. Natl. Acad. Sci. USA 95: 1735). 
     While previously identified markers such as PSA, PSM, PCTA and PSCA have facilitated efforts to diagnose and treat prostate cancer, there is need for the identification of additional markers and therapeutic targets for prostate and related cancers in order to further improve diagnosis and therapy. 
     Renal cell carcinoma (RCC) accounts for approximately 3 percent of adult malignancies. Once adenomas reach a diameter of 2 to 3 cm, malignant potential exists. In the adult, the two principal malignant renal tumors are renal cell adenocarcinoma and transitional cell carcinoma of the renal pelvis or urethras. The incidence of renal cell adenocarcinoma is estimated at more than 29,000 cases in the United States, and more than 11,600 patients died of this disease in 1998. Transitional cell carcinoma is less frequent, with an incidence of approximately 500 cases per year in the United States. 
     Surgery has been the primary therapy for renal cell adenocarcinoma for many decades. Until recently, metastatic disease has been refractory to any systemic therapy. With recent developments in systemic therapies, particularly immunotherapies, metastatic renal cell carcinoma may be approached aggressively in appropriate patients with a possibility of durable responses. Nevertheless, there is a remaining need for effective therapies for these patients. 
     Of all new cases of cancer in the United States, bladder cancer represents approximately 5 percent in men (fifth most common neoplasm) and 3 percent in women (eighth most common neoplasm). The incidence is increasing slowly, concurrent with an increasing older population. In 1998, there was an estimated 54,500 cases, including 39,500 in men and 15,000 in women. The age-adjusted incidence in the United States is 32 per 100,000 for men and eight per 100,000 in women. The historic male/female ratio of 3:1 may be decreasing related to smoking patterns in women. There were an estimated 11,000 deaths from bladder cancer in 1998 (7,800 in men and 3,900 in women). Bladder cancer incidence and mortality strongly increase with age and will be an increasing problem as the population becomes more elderly. 
     Most bladder cancers recur in the bladder. Bladder cancer is managed with a combination of transurethral resection of the bladder (TUR) and intravesical chemotherapy or immunotherapy. The multifocal and recurrent nature of bladder cancer points out the limitations of TUR. Most muscle-invasive cancers are not cured by TUR alone. Radical cystectomy and urinary diversion is the most effective means to eliminate the cancer but carry an undeniable impact on urinary and sexual function. There continues to be a significant need for treatment modalities that are beneficial for bladder cancer patients. 
     An estimated 130,200 cases of colorectal cancer occurred in 2000 in the United States, including 93,800 cases of colon cancer and 36,400 of rectal cancer. Colorectal cancers are the third most common cancers in men and women. Incidence rates declined significantly during 1992-1996 (−2.1% per year). Research suggests that these declines have been due to increased screening and polyp removal, preventing progression of polyps to invasive cancers. There were an estimated 56,300 deaths (47,700 from colon cancer, 8,600 from rectal cancer) in 2000, accounting for about 11% of all U.S. cancer deaths. 
     At present, surgery is the most common form of therapy for colorectal cancer, and for cancers that have not spread, it is frequently curative. Chemotherapy, or chemotherapy plus radiation, is given before or after surgery to most patients whose cancer has deeply perforated the bowel wall or has spread to the lymph nodes. A permanent colostomy (creation of an abdominal opening for elimination of body wastes) is occasionally needed for colon cancer and is infrequently required for rectal cancer. There continues to be a need for effective diagnostic and treatment modalities for colorectal cancer. 
     There were an estimated 164,100 new cases of lung and bronchial cancer in 2000, accounting for 14% of all U.S. cancer diagnoses. The incidence rate of lung and bronchial cancer is declining significantly in men, from a high of 86.5 per 100,000 in 1984 to 70.0 in 1996. In the 1990s, the rate of increase among women began to slow. In 1996, the incidence rate in women was 42.3 per 100,000. 
     Lung and bronchial cancer caused an estimated 156,900 deaths in 2000, accounting for 28% of all cancer deaths. During 1992-1996, mortality from lung cancer declined significantly among men (−1.7% per year) while rates for women were still significantly increasing (0.9% per year). Since 1987, more women have died each year of lung cancer than breast cancer, which, for over 40 years, was the major cause of cancer death in women. Decreasing lung cancer incidence and mortality rates most likely resulted from decreased smoking rates over the previous 30 years; however, decreasing smoking patterns among women lag behind those of men. Of concern, although the declines in adult tobacco use have slowed, tobacco use in youth is increasing again. 
     Treatment options for lung and bronchial cancer are determined by the type and stage of the cancer and include surgery, radiation therapy, and chemotherapy. For many localized cancers, surgery is usually the treatment of choice. Because the disease has usually spread by the time it is discovered, radiation therapy and chemotherapy are often needed in combination with surgery. Chemotherapy alone or combined with radiation is the treatment of choice for small cell lung cancer; on this regimen, a large percentage of patients experience remission, which in some cases is long lasting. There is however, an ongoing need for effective treatment and diagnostic approaches for lung and bronchial cancers. 
     An estimated 182,800 new invasive cases of breast cancer were expected to occur among women in the United States during 2000. Additionally, about 1,400 new cases of breast cancer were expected to be diagnosed in men in 2000. After increasing about 4% per year in the 1980s, breast cancer incidence rates in women have leveled off in the 1990s to about 110.6 cases per 100,000. 
     In the U.S. alone, there were an estimated 41,200 deaths (40,800 women, 400 men) in 2000 due to breast cancer. Breast cancer ranks second among cancer deaths in women. According to the most recent data, mortality rates declined significantly during 1992-1996 with the largest decreases in younger women, both white and black. These decreases were probably the result of earlier detection and improved treatment. 
     Taking into account the medical circumstances and the patient&#39;s preferences, treatment of breast cancer may involve lumpectomy (local removal of the tumor) and removal of the lymph nodes under the arm; mastectomy (surgical removal of the breast) and removal of the lymph nodes under the arm; radiation therapy; chemotherapy; or hormone therapy. Often, two or more methods are used in combination. Numerous studies have shown that, for early stage disease, long-term survival rates after lumpectomy plus radiotherapy are similar to survival rates after modified radical mastectomy. Significant advances in reconstruction techniques provide several options for breast reconstruction after mastectomy. Recently, such reconstruction has been done at the same time as the mastectomy. 
     Local excision of ductal carcinoma in situ (DCIS) with adequate amounts of surrounding normal breast tissue may prevent the local recurrence of the DCIS. Radiation to the breast and/or tamoxifen may reduce the chance of DCIS occurring in the remaining breast tissue. This is important because DCIS, if left untreated, may develop into invasive breast cancer. Nevertheless, there are serious side effects or sequelae to these treatments. There is, therefore, a need for efficacious breast cancer treatments. 
     There were an estimated 23,100 new cases of ovarian cancer in the United States in 2000. It accounts for 4% of all cancers among women and ranks second among gynecologic cancers. During 1992-1996, ovarian cancer incidence rates were significantly declining. Consequent to ovarian cancer, there were an estimated 14,000 deaths in 2000. Ovarian cancer causes more deaths than any other cancer of the female reproductive system. 
     Surgery, radiation therapy, and chemotherapy are treatment options for ovarian cancer. Surgery usually includes the removal of one or both ovaries, the fallopian tubes (salpingo-oophorectomy), and the uterus (hysterectomy). In some very early tumors, only the involved ovary will be removed, especially in young women who wish to have children. In advanced disease, an attempt is made to remove all intra-abdominal disease to enhance the effect of chemotherapy. There continues to be an important need for effective treatment options for ovarian cancer. 
     There were an estimated 28,300 new cases of pancreatic cancer in the United States in 2000. Over the past 20 years, rates of pancreatic cancer have declined in men. Rates among women have remained approximately constant but may be beginning to decline. Pancreatic cancer caused an estimated 28,200 deaths in 2000 in the United States. Over the past 20 years, there has been a slight but significant decrease in mortality rates among men (about −0.9% per year) while rates have increased slightly among women. 
     Surgery, radiation therapy, and chemotherapy are treatment options for pancreatic cancer. These treatment options can extend survival and/or relieve symptoms in many patients but are not likely to produce a cure for most. There is a significant need for additional therapeutic and diagnostic options for pancreatic cancer. 
     SUMMARY OF THE INVENTION 
     The present invention relates to a gene, designated 158P3D2, that has now been found to be over-expressed in the cancer(s) listed in Table I. Northern blot expression analysis of 158P3D2 gene expression in normal tissues shows a restricted expression pattern in adult tissues. The nucleotide ( FIG. 2 ) and amino acid ( FIG. 2 , and  FIG. 3 ) sequences of 158P3D2 are provided. The tissue-related profile of 158P3D2 in normal adult tissues, combined with the over-expression observed in the tissues listed in Table I, shows that 158P3D2 is aberrantly over-expressed in at least some cancers, and thus serves as a useful diagnostic, prophylactic, prognostic, and/or therapeutic target for cancers of the tissue(s) such as those listed in Table I. 
     The invention provides polynucleotides corresponding or complementary to all or part of the 158P3D2 genes, mRNAs, and/or coding sequences, preferably in isolated form, including polynucleotides encoding 158P3D2-related proteins and fragments of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, or more than 25 contiguous amino acids; at least 30, 35, 40, 45, 50, 55, 60, 65, 70, 80, 85, 90, 95, 100 or more than 100 contiguous amino acids of a 158P3D2-related protein, as well as the peptides/proteins themselves; DNA, RNA, DNA/RNA hybrids, and related molecules, polynucleotides or oligonucleotides complementary or having at least a 90% homology to the 158P3D2 genes or mRNA sequences or parts thereof, and polynucleotides or oligonucleotides that hybridize to the 158P3D2 genes, mRNAs, or to 158P3D2-encoding polynucleotides. Also provided are means for isolating cDNAs and the genes encoding 158P3D2. Recombinant DNA molecules containing 158P3D2 polynucleotides, cells transformed or transduced with such molecules, and host-vector systems for the expression of 158P3D2 gene products are also provided. The invention further provides antibodies that bind to 158P3D2 proteins and polypeptide fragments thereof, including polyclonal and monoclonal antibodies, murine and other mammalian antibodies, chimeric antibodies, humanized and fully human antibodies, and antibodies labeled with a detectable marker or therapeutic agent. In certain embodiments, there is a proviso that the entire nucleic acid sequence of  FIG. 2  is not encoded and/or the entire amino acid sequence of  FIG. 2  is not prepared. In certain embodiments, the entire nucleic acid sequence of  FIG. 2  is encoded and/or the entire amino acid sequence of  FIG. 2  is prepared, either of which are in respective human unit dose forms. 
     The invention further provides methods for detecting the presence and status of 158P3D2 polynucleotides and proteins in various biological samples, as well as methods for identifying cells that express 158P3D2. A typical embodiment of this invention provides methods for monitoring 158P3D2 gene products in a tissue or hematology sample having or suspected of having some form of growth dysregulation such as cancer. 
     The invention further provides various immunogenic or therapeutic compositions and strategies for treating cancers that express 158P3D2 such as cancers of tissues listed in Table I, including therapies aimed at inhibiting the transcription, translation, processing or function of 158P3D2 as well as cancer vaccines. In one aspect, the invention provides compositions, and methods comprising them, for treating a cancer that expresses 158P3D2 in a human subject wherein the composition comprises a carrier suitable for human use and a human unit dose of one or more than one agent that inhibits the production or function of 158P3D2. Preferably, the carrier is a uniquely human carrier. In another aspect of the invention, the agent is a moiety that is immunoreactive with 158P3D2 protein. Non-limiting examples of such moieties include, but are not limited to, antibodies (such as single chain, monoclonal, polyclonal, humanized, chimeric, or human antibodies), functional equivalents thereof (whether naturally occurring or synthetic), and combinations thereof. The antibodies can be conjugated to a diagnostic or therapeutic moiety. In another aspect, the agent is a small molecule as defined herein. 
     In another aspect, the agent comprises one or more than one peptide which comprises a cytotoxic T lymphocyte (CTL) epitope that binds an HLA class I molecule in a human to elicit a CTL response to 158P3D2 and/or one or more than one peptide which comprises a helper T lymphocyte (HTL) epitope which binds an HLA class II molecule in a human to elicit an HTL response. The peptides of the invention may be on the same or on one or more separate polypeptide molecules. In a further aspect of the invention, the agent comprises one or more than one nucleic acid molecule that expresses one or more than one of the CTL or HTL response stimulating peptides as described above. In yet another aspect of the invention, the one or more than one nucleic acid molecule may express a moiety that is immunologically reactive with 158P3D2 as described above. The one or more than one nucleic acid molecule may also be, or encodes, a molecule that inhibits production of 158P3D2. Non-limiting examples of such molecules include, but are not limited to, those complementary to a nucleotide sequence essential for production of 158P3D2 (e.g. antisense sequences or molecules that form a triple helix with a nucleotide double helix essential for 158P3D2 production) or a ribozyme effective to lyse 158P3D2 mRNA. 
     Note that to determine the starting position of any peptide set forth in Tables VIII-XXI and XXII to XLIX (collectively HLA Peptide Tables) respective to its parental protein, e.g., variant 1, variant 2, etc., reference is made to three factors: the particular variant, the length of the peptide in an HLA Peptide Table, and the Search Peptides in Table VII. Generally, a unique Search Peptide is used to obtain HLA peptides of a particular for a particular variant. The position of each Search Peptide relative to its respective parent molecule is listed in Table VII. Accordingly, if a Search Peptide begins at position “X”, one must add the value “X−1” to each position in Tables VIII-XXI and XXII to XLIX to obtain the actual position of the HLA peptides in their parental molecule. For example, if a particular Search Peptide begins at position 150 of its parental molecule, one must add 150−1, i.e., 149 to each HLA peptide amino acid position to calculate the position of that amino acid in the parent molecule. 
     One embodiment of the invention comprises an HLA peptide, that occurs at least twice in Tables VIII-XXI and XXII to XLIX collectively, or an oligonucleotide that encodes the HLA peptide. Another embodiment of the invention comprises an HLA peptide that occurs at least once in Tables VIII-XXI and at least once in tables XXII to XLIX, or an oligonucleotide that encodes the HLA peptide. 
     Another embodiment of the invention is antibody epitopes, which comprise a peptide regions, or an oligonucleotide encoding the peptide region, that has one two, three, four, or five of the following characteristics: 
     i) a peptide region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or greater than 0.5, 0.6, 0.7, 0.8, 0.9, or having a value equal to 1.0, in the Hydrophilicity profile of  FIG. 5 ; 
     ii) a peptide region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or less than 0.5, 0.4, 0.3, 0.2, 0.1, or having a value equal to 0.0, in the Hydropathicity profile of  FIG. 6 ; 
     iii) a peptide region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or greater than 0.5, 0.6, 0.7, 0.8, 0.9, or having a value equal to 1.0, in the Percent Accessible Residues profile of  FIG. 7 ; 
     iv) a peptide region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or greater than 0.5, 0.6, 0.7, 0.8, 0.9, or having a value equal to 1.0, in the Average Flexibility profile of  FIG. 8 ; or 
     v) a peptide region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or greater than 0.5, 0.6, 0.7, 0.8, 0.9, or having a value equal to 1.0, in the Beta-turn profile of  FIG. 9 . [!!!The Figure descriptions need to be revised when using this as a template] 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1 . The 158P3D2 SSH sequence of 312 nucleotides. 
         FIG. 2A ) The cDNA and amino acid sequence of 158P3D2 variant 1 clone 158P3D2-BCP-1 (also called “158P3D2 v.1” or “158P3D2 variant 1”) is shown in  FIG. 2A . The start methionine is underlined. The open reading frame extends from nucleic acid 849-1835 including the stop codon. 
         FIG. 2B ) The cDNA and amino acid sequence of 158P3D2 variant 2A (also called “158P3D2 v.2”) is shown in  FIG. 2B . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 117-827 including the stop codon. 
         FIG. 2C ) The cDNA and amino acid sequence of 158P3D2 variant 2B (also called “158P3D2 v.2”) is shown in  FIG. 2C . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 2249-2794 including the stop codon. 
         FIG. 2D ) The cDNA and amino acid sequence of 158P3D2 variant 3 (also called “158P3D2 v.3”) is shown in  FIG. 2D . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 849-1835 including the stop codon. 
         FIG. 2E ) The cDNA and amino acid sequence of 158P3D2 variant 4 (also called “158P3D2 v.4”) is shown in  FIG. 2E . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 849-1835 including the stop codon. 
         FIG. 2F ) The cDNA and amino acid sequence of 158P3D2 variant 5A (also called “158P3D2 v.5”) is shown in  FIG. 2F . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 849-1385 including the stop codon. 
         FIG. 2G ) The cDNA and amino acid sequence of 158P3D2 variant 5B (also called “158P3D2 v.5”) is shown in  FIG. 2G . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 1289-1834 including the stop codon. 
         FIG. 2H ) The cDNA and amino acid sequence of 158P3D2 variant 6 (also called “158P3D2 v.6”) is shown in  FIG. 2H . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 849-1835 including the stop codon. 
         FIG. 2I ) The cDNA and amino acid sequence of 158P3D2 variant 7 (also called “158P3D2 v.7”) is shown in  FIG. 2I . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 849-1835 including the stop codon. 
         FIG. 2J ) The cDNA and amino acid sequence of 158P3D2 variant 8 (also called “158P3D2 v.8”) is shown in  FIG. 2J . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 849-1835 including the stop codon. 
         FIG. 2K ) The cDNA and amino acid sequence of 158P3D2 variant 14 (also called “158P3D2 v.14”) is shown in  FIG. 2K . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 65-4246 including the stop codon. 
         FIG. 2L ) The cDNA and amino acid sequence of 158P3D2 variant 15 (also called “158P3D2 v.15”) is shown in  FIG. 2L . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 65-3502 including the stop codon. 
         FIG. 2M ) The cDNA and amino acid sequence of 158P3D2 variant 16 (also called “158P3D2 v.16”) is shown in  FIG. 2M . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 65-6037 including the stop codon. 
         FIG. 2N ) The cDNA and amino acid sequence of 158P3D2 variant 17 (also called “158P3D2 v.17”) is shown in  FIG. 2N . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 65-6175 including the stop codon. 
         FIG. 2O ) The cDNA and amino acid sequence of 158P3D2 variant 18 (also called “158P3D2 v.18”) is shown in  FIG. 2O . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 2932-4764 including the stop codon. 
         FIG. 2P ) The cDNA and amino acid sequence of 158P3D2 variant 19 (also called “158P3D2 v.19”) is shown in  FIG. 2P . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 65-6001 including the stop codon. 
         FIG. 2Q ) The cDNA and amino acid sequence of 158P3D2 variant 20 (also called “158P3D2 v.20”) is shown in  FIG. 2Q . The codon for the start methionine is underlined. The open reading frame extends from nucleic acid 65-6121 including the stop codon. 
         FIG. 2R ) 158P3D2 v.9 through v.13, SNP variants of 158P3D2 v.1. The 158P3D2 v.9 through v.13 proteins have 1072 amino acids. Variants 158P3D2 v.4 through v.20 are variants with single nucleotide difference from 158P3D2 v.1. 158P3D2 v.10, v.12 and v.13 proteins differ from 158P3D2 v.1 by one amino acid. 158P3D2 v.9 and v.11 proteins code for the same protein as v.1. Though these SNP variants are shown separately, they can also occur in any combinations and in any of the transcript variants listed above in  FIGS. 2A-2Q . 
         FIG. 3A ) The amino acid sequence of 158P3D2 v.1 clone 158P3D2-BCP-1 is shown in  FIG. 3A ; it has 328 amino acids. 
         FIG. 3B ) The amino acid sequence of 158P3D2 v.2A is shown in  FIG. 3B ; it has 236 amino acids. 
         FIG. 3C ) The amino acid sequence of 158P3D2 v.2B is shown in  FIG. 3C ; it has 181 amino acids. 
         FIG. 3D ) The amino acid sequence of 158P3D2 v.3 is shown in  FIG. 3D ; it has 328 amino acids. 
         FIG. 3E ) The amino acid sequence of 158P3D2 v.4 is shown in  FIG. 3E ; it has 328 amino acids. 
         FIG. 3F ) The amino acid sequence of 158P3D2 v.5A is shown in  FIG. 3F ; it has 178 amino acids. 
         FIG. 3G ) The amino acid sequence of 158P3D2 v.5B is shown in  FIG. 3G ; it has 181 amino acids. 
         FIG. 3H ) The amino acid sequence of 158P3D2 v.10 is shown in  FIG. 3H ; it has 328 amino acids. 
         FIG. 3I ) The amino acid sequence of 158P3D2 v.11 is shown in  FIG. 3I ; it has 328 amino acids. 
         FIG. 3J ) The amino acid sequence of 158P3D2 v.12 is shown in  FIG. 3J ; it has 328 amino acids. 
         FIG. 3K ) The amino acid sequence of 158P3D2 v.13 is shown in  FIG. 3K ; it has 328 amino acids. 
         FIG. 3L ) The amino acid sequence of 158P3D2 v.14 is shown in  FIG. 3L ; it has 1393 amino acids. 
         FIG. 3M ) The amino acid sequence of 158P3D2 v.15 is shown in  FIG. 3M ; it has 1145 amino acids. 
         FIG. 3N ) The amino acid sequence of 158P3D2 v.16 is shown in  FIG. 3N ; it has 1990 amino acids. 
         FIG. 3O ) The amino acid sequence of 158P3D2 v.17 is shown in  FIG. 3O ; it has 2036 amino acids. 
         FIG. 3P ) The amino acid sequence of 158P3D2 v.18 is shown in  FIG. 3P ; it has 610 amino acids. 
         FIG. 3Q ) The amino acid sequence of 158P3D2 v.19 is shown in  FIG. 3Q ; it has 1978 amino acids. 
         FIG. 3R ) The amino acid sequence of 158P3D2 v.20 is shown in  FIG. 3R ; it has 2018 amino acids. 
       As used herein, a reference to 158P3D2 includes all variants thereof, including those shown in  FIGS. 2 ,  3 ,  10 ,  11 , and  12  unless the context clearly indicates otherwise. 
         FIG. 4 . Effect of 158P3D2 RNAi on cell proliferation. SCaBER cells or Cos-1 cells were transfected with Lipofectamine 2000 reagent (LF2K) alone, or with negative control Luc4 oligo (20 nM), positive control Eg5 oligo (20 nM) or 158P3D2.b oligo (20 nM). After 48 hours, the media was replaced and the cells were incubated for 24 hrs, pulsed with  3 H-thymidine at 1.5 μCi/ml for 14 hrs, harvested onto a filtermat and counted in scintillation cocktail on a Microbeta trilux counter. Percentage cell proliferation relative to the LF2k control (100%) is shown. The reduction in 158P3D2 levels by the 158P3D2.b siRNA oligo correlated with diminished cell proliferation in the SCaBER cells, but no effect was observed in the 158P3D2-negative cell line Cos-1. 
         FIG. 5(   a )-( i ). Hydrophilicity amino acid profile of 158P3D2 v.1, v.2a, v.2b, v.5a, v.14, v.15, v.16, v.17, and v.18 determined by computer algorithm sequence analysis using the method of Hopp and Woods (Hopp T. P., Woods K. R., 1981. Proc. Natl. Acad. Sci. U.S.A. 78:3824-3828) accessed on the Protscale website located on the World Wide Web at (expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server. 
         FIG. 6(   a )-( i ). Hydropathicity amino acid profile of 158P3D2 v.1, v.2a, v.2b, v.5a, v.14, v.15, v.16, v.17, and v.18 determined by computer algorithm sequence analysis using the method of Kyte and Doolittle (Kyte J., Doolittle R. F., 1982. J. Mol. Biol. 157:105-132) accessed on the ProtScale website located on the World Wide Web at (.expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server. 
         FIG. 7(   a )-( i ). Percent accessible residues amino acid profile of 158P3D2 v.1, v.2a, v.2b, v.5a, v.14, v.15, v.16, v.17, and v.18 determined by computer algorithm sequence analysis using the method of Janin (Janin J., 1979 Nature 277:491-492) accessed on the ProtScale website located on the World Wide Web at (.expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server. 
         FIG. 8(   a )-( i ). Average flexibility amino acid profile of 158P3D2 v.1, v.2a, v.2b, v.5a, v.14, v.15, v.16, v.17, and v.18 determined by computer algorithm sequence analysis using the method of Bhaskaran and Ponnuswamy (Bhaskaran R., and Ponnuswamy P. K., 1988. Int. J. Pept. Protein Res. 32:242-255) accessed on the ProtScale website located on the World Wide Web at (.expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server. 
         FIG. 9(   a )-( i ). Beta-turn amino acid profile of 158P3D2 v.1, v.2a, v.2b, v.5a, v.14, v.15, v.16, v.17, and v.18 determined by computer algorithm sequence analysis using the method of Deleage and Roux (Deleage, G., Roux B. 1987 Protein Engineering 1:289-294) accessed on the ProtScale website located on the World Wide Web at (.expasy.ch/cgi-bin/protscale.pl) through the ExPasy molecular biology server. 
         FIG. 10 . Exon compositions of transcript variants of 158P3D2. Variant 158P3D2 v.2, v.14 through v.20 are transcript variants. Compared with 158P3D2 v.1; v.2 had six additional exons to the 5′ end, an exon 7 longer than exon 1 of 158P3D2 v.1 and an exon 10 shorter than exon 4 of 158P3D2 v.1. Exons 2, 3, 5, 6 and 7 of 158P3D2 v.1 are the same as exons 8, 9, 11, 12 and 13 of 158P3D2 v.2, respectively. Other variants had different exon compositions as shown above. Numbers in “( )” underneath the box correspond to those of 158P3D2 v.1. Black boxes show the same sequence as 158P3D2 v.1. Length of introns are not proportional. 
         FIG. 11 . Schematic display of protein variants of 158P3D2. Nucleotide variant 158P3D2 v.2 and 158P3D2 v.5 potentially coded for two different proteins, designated as variants 158P3D2 v.2A and 158P3D2 v.2B, 158P3D2 v.5A and 158P3D2 v.5B, respectively. Variant 158P3D2 v.5B shares the same amino acid sequence as variant 158P3D2 v.2B. Variants 158P3D2 v.3 and v.4 were variants with single amino acid variations. Black box shows the same sequence as 158P3D2 v.1. Numbers in “( )” underneath the black boxes correspond to those of 158P3D2 v.1 and those underneath the “brick” boxes correspond to those of v.17. Single amino acid differences are indicated above the box. 
         FIG. 12 . Schematic display of SNP variants of 158P3D2. Variant 158P3D2 v.3 through v.13 are variants with a single nucleotide difference from v.1. Though these alternative SNP alleles were shown separately, they could occur in any transcript variants in any combination (called haplotype). Numbers in “( )” underneath the box correspond to those of 158P3D2 v.1. ‘-’ indicate single nucleotide deletion. Black boxes show the same sequence as 158P3D2 v.1. SNPs are indicated above the box. 
         FIG. 13 . Secondary structure and transmembrane domains prediction for 158P3D2 protein variants. 
         FIG. 13A  (SEQ ID NO:54),  FIG. 13B  (SEQ ID NO:55),  FIG. 13C  (SEQ ID NO:56),  FIG. 13D  (SEQ ID NO:57),  FIG. 13E  (SEQ ID NO:58),  FIG. 13F  (SEQ ID NO:59),  FIG. 13G  (SEQ ID NO:60),  FIG. 13H  (SEQ ID NO:61),  FIG. 13I  (SEQ ID NO:62): The secondary structures of 158P3D2 protein variants 1, 2a, 2b, 5a, 14, 15, 16, 17, 18 respectively, were predicted using the HNN—Hierarchical Neural Network method (NPS@: Network Protein Sequence Analysis TIBS 2000 March Vol. 25, No 3 [291]:147-150 Combet C., Blanchet C., Geourjon C. and Deleage G., accessed from the ExPasy molecular biology server. This method predicts the presence and location of alpha helices, extended strands, and random coils from the primary protein sequence. The percent of the protein variant in a given secondary structure is also listed. 
         FIG. 13J ,  FIG. 13L ,  FIG. 13N ,  FIG. 13P ,  FIG. 13R ,  FIG. 13T ,  FIG. 13V ,  FIG. 13X , and  FIG. 13Z : Schematic representation of the probability of existence of transmembrane regions of 158P3D2 protein variants 1, 2a, 2b, 5a, 14, 15, 16, 17, 18 respectively, based on the TMpred algorithm of Hofmann and Stoffel which utilizes TMBASE (K. Hofmann, W. Stoffel. TMBASE—A database of membrane spanning protein segments Biol. Chem. Hoppe-Seyler 374:166, 1993).  FIG. 13K ,  FIG. 13M ,  FIG. 13O ,  FIG. 13Q ,  FIG. 13S ,  FIG. 13U ,  FIG. 13W ,  FIG. 13Y , FIG.  13 AA: Schematic representation of the probability of the existence of transmembrane regions of 158P3D2 variants 1, 2a, 2b, 5a, 14, 15, 16, 17, 18 respectively, based on the TMHMM algorithm of Sonnhammer, von Heijne, and Krogh (Erik L. L. Sonnhammer, Gunnar von Heijne, and Anders Krogh: A hidden Markov model for predicting transmembrane helices in protein sequences. In Proc. of Sixth Int. Conf. on Intelligent Systems for Molecular Biology, p 175-182 Ed J. Glasgow, T. Littlejohn, F. Major, R. Lathrop, D. Sankoff, and C. Sensen Menlo Park, Calif.: AAAI Press, 1998). The TMpred and TMHMM algorithms are accessed from the ExPasy molecular biology server. 
         FIG. 14 . 158P3D2 Expression in Normal and Cancer Tissue Specimens. First strand cDNA was prepared from a panel of 13 normal tissues (brain, heart, kidney, liver, lung, spleen, skeletal muscle, testis, pancreas, colon, stomach) and pools of 4-7 patients from the following cancer indications: bladder, kidney, colon, lung, pancreas, stomach, ovary, breast, multiple cancer metastasis, cervix, lymphoma as well as from a pool of patient-derived xenografts (prostate cancer, bladder cancer and kidney cancer). Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Samples were run on an agarose gel, and PCR products were quantitated using the AlphaImager software. Results show strong expression of 158P3D2 in cancers of the bladder, kidney, colon, lung, pancreas, stomach, ovary, breast, cervix, and lymphoma. Low expression was detected in all normal tissues tested except in normal stomach. Strong expression was also observed in the cancer metastasis pool. 
         FIG. 15 . 158P3D2 Expression in bladder cancer patient specimens. First strand cDNA was prepared from normal bladder, bladder cancer cell lines (UM-UC-3, TCCSUP, J82) and a panel of bladder cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show expression of 158P3D2 in the majority of bladder cancer patient specimens tested. Very low expression was detected in normal tissues, but no expression was seen in the cell lines tested. 
         FIG. 16 . 158P3D2 Expression in bladder cancer patient specimens by northern blotting. RNA was extracted from normal bladder, bladder cancer cell lines (UM-UC-3, J82, SCaBER), bladder cancer patient tumors (T) and their normal adjacent tissues (NAT). Northern blot with 10 ug of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor tissues, but not in normal nor NAT tissues. 
         FIG. 17 . 158P3D2 Expression in lung cancer patient specimens. First strand cDNA was prepared from normal lung, cancer cell line A427 and a panel of lung cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). 158P3D2 is expressed at varying levels in 35/39 (90%) of lung cancer specimens, but not in all 3 normal lung tissues tested. 
         FIG. 18 . 158P3D2 Expression in lung cancer patient specimens by northern blotting. RNA was extracted from normal lung, A427 lung cancer cell line, and a panel of lung cancer patient specimens. Northern blot with 10 ug of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor specimens but not in normal tissues. 
         FIG. 19 . 158P3D2 Expression in cancer metastasis patient specimens. First strand cDNA was prepared from normal colon, kidney, liver, lung, pancreas, stomach and from a panel of cancer metastasis patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show expression of 158P3D2 in the majority of patient cancer metastasis specimens tested but not in normal tissues. 
         FIG. 20 . 158P3D2 Expression in cervical cancer patient specimens. First strand cDNA was prepared from normal cervix, cervical cancer cell line HeLa, and a panel of cervical cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show expression of 158P3D2 in all 14 cervical cancer patient specimens tested. No expression was detected in normal cervix nor in the cell line tested. 
         FIG. 21 . 158P3D2 Expression in cervical cancer patient specimens by northern blotting. RNA was extracted from normal cervix, cervical cancer cell line HeLa, and a panel of cervical cancer patient specimens. Northern blot with 10 ug of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor tissues, but not in normal cervix nor in the cell line. 
         FIG. 22 . 158P3D2 Expression in kidney cancer patient specimens. First strand cDNA was prepared from normal kidney, kidney cancer cell lines (769-P, A-498, CAM-1), and a panel of kidney cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). 158P3D2 is expressed at varying levels in the majority of kidney cancer patient specimens, but not in all 3 normal kidney tissues tested. Low expression was detected in 2 of 3 cell lines tested. 
         FIG. 23 . 158P3D2 Expression in kidney cancer patient specimens by northern blotting. RNA was extracted from normal kidney and a panel of kidney cancer patient specimens. Northern blot with 10 ug of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor specimens but not in the normal tissue. 
         FIG. 24 . 158P3D2 Expression in stomach cancer patient specimens. First strand cDNA was prepared from normal stomach, and a panel of stomach cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). 158P3D2 is expressed at varying levels in the majority of stomach cancer patient specimens. Weak expression was detected in the 2 normal stomach, and only in 1 of the 2 NAT tissues tested. 
         FIG. 25 . 158P3D2 Expression in stomach cancer patient specimens by northern blotting. RNA was extracted from normal stomach and a panel of stomach cancer patient specimens. Northern blot with 10 ug of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor specimens but not in the normal tissue. 
         FIG. 26 . 158P3D2 Expression in colon cancer patient specimens. First strand cDNA was prepared from normal colon, colon cancer cell lines (LoVo, CaCO-2, SK CO 1, Colo 205, T284), and a panel of colon cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). 158P3D2 is expressed at varying levels in the majority of colon cancer patient specimens. But it was weakly expressed in just 2 of 3 normal tissues, and 3 of 5 cell lines tested. 
         FIG. 27 . 158P3D2 Expression in uterus cancer patient specimens. First strand cDNA was prepared from normal uterus and a panel of uterus cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show 158P3D2 is expressed at varying levels in the majority of uterus cancer patient specimens, but not in normal uterus. 
         FIG. 28 . 158P3D2 Expression in breast cancer patient specimens. First strand cDNA was prepared from normal breast, breast cancer cell lines (MD-MBA-435S, DU4475, MCF-7, CAMA-1, MCF10A), and a panel of breast cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show 158P3D2 is expressed at varying levels in the majority of breast cancer patient specimens. But it was weakly expressed in just 2 of 3 normal tissues, and 2 of 5 cell lines tested. 
         FIG. 29 . Serum titer of mice immunized with KLH-peptide encoding amino acids 315-328 of 158P3D2. Serial dilutions of serum taken from immunized mice were incubated on an ELISA plate coated with the 158P3 D2 peptide conjugated to ovalbumin Specific bound antibody was then detected by incubation goat anti-mouse IgG-HRP conjugate and then visualized and quantitated by development with TMB substrate and optical density determination. 
         FIG. 30 . Validation of 158P3D2 siRNA oligo. Cos-1 cells were transfected with 1 μg pcDNA3-158P3D2, which encodes a full-length 158P3D2 protein fusion with a Myc/His tag on the C-terminus, simultaneously with Lipofectamine 2000 reagent (LF2k) alone, or with control CT1 oligo (20 nM), 158P3D2.b oligo (20 nM), or no DNA or oligo (No DNA). After 72 hours, the cells were lysed in 1% Triton buffer, and 50 μg of total soluble cell lysate was analyzed by Western blotting. The upper panel was blotted with anti-Myc (1:1000) to detect the 158P3D2-Myc/His fusion protein and the lower panel was developed with anti-actin. The level of 158P3D2 was diminished by the 158P3D2 siRNA oligo, whereas no change was observed with the control (LF2k) or siRNA oligo CT1. In contrast, no change in the level of actin was noted in the cell lysates, indicating that the loading was equivalent in all lanes. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Outline of Sections 
     I.) Definitions 
     II.) 158P3D2 Polynucleotides 
     II.A.) Uses of 158P3D2 Polynucleotides 
     II.A.1.) Monitoring of Genetic Abnormalities 
     II.A.2.) Antisense Embodiments 
     II.A.3.) Primers and Primer Pairs 
     II.A.4.) Isolation of 158P3D2-Encoding Nucleic Acid Molecules 
     II.A.5.) Recombinant Nucleic Acid Molecules and Host-Vector Systems 
     III.) 158P3D2-related Proteins 
     III.A.) Motif-bearing Protein Embodiments 
     III.B.) Expression of 158P3D2-related Proteins 
     III.C.) Modifications of 158P3D2-related Proteins 
     III.D.) Uses of 158P3D2-related Proteins 
     IV.) 158P3D2 Antibodies 
     V.) 158P3D2 Cellular Immune Responses 
     VI.) 158P3D2 Transgenic Animals 
     VII.) Methods for the Detection of 158P3D2 
     VIII.) Methods for Monitoring the Status of 158P3D2-related Genes and Their Products 
     IX.) Identification of Molecules That Interact With 158P3D2 
     X.) Therapeutic Methods and Compositions 
     X.A.) Anti-Cancer Vaccines 
     X.B.) 158P3D2 as a Target for Antibody-Based Therapy 
     X.C.) 158P3D2 as a Target for Cellular Immune Responses 
     X.C.1 Minigene Vaccines 
     X.C.2. Combinations of CTL Peptides with Helper Peptides 
     X.C.3. Combinations of CTL Peptides with T Cell Priming Agents 
     X.C.4. Vaccine Compositions Comprising DC Pulsed with CTL and/or HTL Peptides 
     X.D.) Adoptive Immunotherapy 
     X.E.) Administration of Vaccines for Therapeutic or Prophylactic Purposes 
     XI.) Diagnostic and Prognostic Embodiments of 158P3D2. 
     XII.) Inhibition of 158P3D2 Protein Function 
     XII.A.) Inhibition of 158P3D2 With Intracellular Antibodies 
     XII.B.) Inhibition of 158P3D2 with Recombinant Proteins 
     XII.C.) Inhibition of 158P3D2 Transcription or Translation 
     XII.D.) General Considerations for Therapeutic Strategies 
     XIII.) Identification, Characterization and Use of Modulators of 109P1D1 
     XIII.A.) Methods to Identify and Use Modulators 
     XIII.B.) Gene Expression-related Assays 
     XIII.C.) Expression Monitoring to Identify Compounds that Modify Gene Expression 
     XIII.D.) Biological Activity-related Assays 
     XIII.E.) High Throughput Screening to Identify Modulators 
     XIII.F.) Use of Soft Agar Growth and Colony Formation to Identify and Characterize Modulators 
     XIII.G.) Evaluation of Contact Inhibition and Growth Density Limitation to Identify and Characterize Modulators 
     XIII.H.) Evaluation of Growth Factor or Serum Dependence to Identify and Characterize Modulators 
     XIII.I.) Use of Tumor-specific Marker Levels to Identify and Characterize Modulators 
     XIII.J.) Invasiveness into Matrigel to Identify and Characterize Modulators 
     XIII.K.) Evaluation of Tumor Growth In Vivo to Identify and Characterize Modulators 
     XIII.L.) In Vitro Assays to Identify and Characterize Modulators 
     XIII.M.) Binding Assays to Identify and Characterize Modulators 
     XIII.N.) Competitive Binding to Identify and Characterize Modulators 
     XIII.O.) Use of Polynucleotides to Down-regulate or Inhibit a Protein of the Invention. 
     XIII.P.) Inhibitory and Antisense Nucleotides 
     XIII.Q.) Ribozymes 
     XIII.R.) Use of Modulators in Phenotypic Screening 
     XIII.S.) Use of Modulators to Affect Peptides of the Invention 
     XIII.T.) Methods of Identifying Characterizing Cancer-associated Sequences 
     XIV.) KITS/Articles of Manufacture 
     I.) Definitions 
     Unless otherwise defined, all terms of art, notations and other scientific terms or terminology used herein are intended to have the meanings commonly understood by those of skill in the art to which this invention pertains. In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference, and the inclusion of such definitions herein should not necessarily be construed to represent a substantial difference over what is generally understood in the art. Many of the techniques and procedures described or referenced herein are well understood and commonly employed using conventional methodology by those skilled in the art, such as, for example, the widely utilized molecular cloning methodologies described in Sambrook et al., Molecular Cloning: A Laboratory Manual 2nd. edition (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. As appropriate, procedures involving the use of commercially available kits and reagents are generally carried out in accordance with manufacturer defined protocols and/or parameters unless otherwise noted. 
     The terms “advanced prostate cancer”, “locally advanced prostate cancer”, “advanced disease” and “locally advanced disease” mean prostate cancers that have extended through the prostate capsule, and are meant to include stage C disease under the American Urological Association (AUA) system, stage C1-C2 disease under the Whitmore-Jewett system, and stage T3-T4 and N+ disease under the TNM (tumor, node, metastasis) system. In general, surgery is not recommended for patients with locally advanced disease, and these patients have substantially less favorable outcomes compared to patients having clinically localized (organ-confined) prostate cancer. Locally advanced disease is clinically identified by palpable evidence of induration beyond the lateral border of the prostate, or asymmetry or induration above the prostate base. Locally advanced prostate cancer is presently diagnosed pathologically following radical prostatectomy if the tumor invades or penetrates the prostatic capsule, extends into the surgical margin, or invades the seminal vesicles. 
     “Altering the native glycosylation pattern” is intended for purposes herein to mean deleting one or more carbohydrate moieties found in native sequence 158P3D2 (either by removing the underlying glycosylation site or by deleting the glycosylation by chemical and/or enzymatic means), and/or adding one or more glycosylation sites that are not present in the native sequence 158P3D2. In addition, the phrase includes qualitative changes in the glycosylation of the native proteins, involving a change in the nature and proportions of the various carbohydrate moieties present. 
     The term “analog” refers to a molecule which is structurally similar or shares similar or corresponding attributes with another molecule (e.g. a 158P3D2-related protein). For example, an analog of a 158P3D2 protein can be specifically bound by an antibody or T cell that specifically binds to 158P3D2. 
     The term “antibody” is used in the broadest sense. Therefore, an “antibody” can be naturally occurring or man-made such as monoclonal antibodies produced by conventional hybridoma technology. Anti-158P3D2 antibodies comprise monoclonal and polyclonal antibodies as well as fragments containing the antigen-binding domain and/or one or more complementarity determining regions of these antibodies. 
     An “antibody fragment” is defined as at least a portion of the variable region of the immunoglobulin molecule that binds to its target, i.e., the antigen-binding region. In one embodiment it specifically covers single anti-158P3D2 antibodies and clones thereof (including agonist, antagonist and neutralizing antibodies) and anti-158P3D2 antibody compositions with polyepitopic specificity. 
     The term “codon optimized sequences” refers to nucleotide sequences that have been optimized for a particular host species by replacing any codons having a usage frequency of less than about 20%. Nucleotide sequences that have been optimized for expression in a given host species by elimination of spurious polyadenylation sequences, elimination of exon/intron splicing signals, elimination of transposon-like repeats and/or optimization of GC content in addition to codon optimization are referred to herein as an “expression enhanced sequences.” 
     A “combinatorial library” is a collection of diverse chemical compounds generated by either chemical synthesis or biological synthesis by combining a number of chemical “building blocks” such as reagents. For example, a linear combinatorial chemical library, such as a polypeptide (e.g., mutein) library, is formed by combining a set of chemical building blocks called amino acids in every possible way for a given compound length (i.e., the number of amino acids in a polypeptide compound). Numerous chemical compounds are synthesized through such combinatorial mixing of chemical building blocks (Gallop et al., J. Med. Chem. 37(9): 1233-1251 (1994)). 
     Preparation and screening of combinatorial libraries is well known to those of skill in the art. Such combinatorial chemical libraries include, but are not limited to, peptide libraries (see, e.g., U.S. Pat. No. 5,010,175, Furka, Pept. Prot. Res. 37:487-493 (1991), Houghton et al., Nature, 354:84-88 (1991)), peptoids (PCT Publication No WO 91/19735), encoded peptides (PCT Publication WO 93/20242), random bio-oligomers (PCT Publication WO 92/00091), benzodiazepines (U.S. Pat. No. 5,288,514), diversomers such as hydantoins, benzodiazepines and dipeptides (Hobbs et al., Proc. Nat. Acad. Sci. USA 90:6909-6913 (1993)), vinylogous polypeptides (Hagihara et al., J. Amer. Chem. Soc. 114:6568 (1992)), nonpeptidal peptidomimetics with a Beta-D-Glucose scaffolding (Hirschmann et al., J. Amer. Chem. Soc. 114:9217-9218 (1992)), analogous organic syntheses of small compound libraries (Chen et al., J. Amer. Chem. Soc. 116:2661 (1994)), oligocarbamates (Cho, et al., Science 261:1303 (1993)), and/or peptidyl phosphonates (Campbell et al., J. Org. Chem. 59:658 (1994)). See, generally, Gordon et al., J. Med. Chem. 37:1385 (1994), nucleic acid libraries (see, e.g., Stratagene, Corp.), peptide nucleic acid libraries (see, e.g., U.S. Pat. No. 5,539,083), antibody libraries (see, e.g., Vaughn et al., Nature Biotechnology 14(3): 309-314 (1996), and PCT/US96/10287), carbohydrate libraries (see, e.g., Liang et al., Science 274:1520-1522 (1996), and U.S. Pat. No. 5,593,853), and small organic molecule libraries (see, e.g., benzodiazepines, Baum, C&amp;EN, January 18, page 33 (1993); isoprenoids, U.S. Pat. No. 5,569,588; thiazolidinones and metathiazanones, U.S. Pat. No. 5,549,974; pyrrolidines, U.S. Pat. Nos. 5,525,735 and 5,519,134; morpholino compounds, U.S. Pat. No. 5,506,337; benzodiazepines, U.S. Pat. No. 5,288,514; and the like). 
     Devices for the preparation of combinatorial libraries are commercially available (see, e.g., 357 NIPS, 390 NIPS, Advanced Chem Tech, Louisville Ky.; Symphony, Rainin, Woburn, Mass.; 433A, Applied Biosystems, Foster City, Calif.; 9050, Plus, Millipore, Bedford, NIA). A number of well-known robotic systems have also been developed for solution phase chemistries. These systems include automated workstations such as the automated synthesis apparatus developed by Takeda Chemical Industries, LTD. (Osaka, Japan) and many robotic systems utilizing robotic arms (Zymate H, Zymark Corporation, Hopkinton, Mass.; Orca, Hewlett-Packard, Palo Alto, Calif.), which mimic the manual synthetic operations performed by a chemist. Any of the above devices are suitable for use with the present invention. The nature and implementation of modifications to these devices (if any) so that they can operate as discussed herein will be apparent to persons skilled in the relevant art. In addition, numerous combinatorial libraries are themselves commercially available (see, e.g., ComGenex, Princeton, N.J.; Asinex, Moscow, RU; Tripos, Inc., St. Louis, Mo.; ChemStar, Ltd, Moscow, RU; 3D Pharmaceuticals, Exton, Pa.; Martek Biosciences, Columbia, Md.; etc.). 
     The term “cytotoxic agent” refers to a substance that inhibits or prevents the expression activity of cells, function of cells and/or causes destruction of cells. The term is intended to include radioactive isotopes chemotherapeutic agents, and toxins such as small molecule toxins or enzymatically active toxins of bacterial, fungal, plant or animal origin, including fragments and/or variants thereof. Examples of cytotoxic agents include, but are not limited to auristatins, auromycins, maytansinoids, yttrium, bismuth, ricin, ricin A-chain, combrestatin, duocarmycins, dolostatins, doxorubicin, daunorubicin, taxol, cisplatin, cc1065, ethidium bromide, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicine, dihydroxy anthracin dione, actinomycin, diphtheria toxin,  Pseudomonas  exotoxin (PE) A, PE40, abrin, abrin A chain, modeccin A chain, alpha-sarcin, gelonin, mitogellin, retstrictocin, phenomycin, enomycin, curicin, crotin, calicheamicin, Sapaonaria officinalis inhibitor, and glucocorticoid and other chemotherapeutic agents, as well as radioisotopes such as At211, I131, I125, Y90, Rr186, Re188, Sm153, Bi212 or 213, P32 and radioactive isotopes of Lu including Lu177. Antibodies may also be conjugated to an anti-cancer pro-drug activating enzyme capable of converting the pro-drug to its active form. 
     The “gene product” is sometimes referred to herein as a protein or mRNA. For example, a “gene product of the invention” is sometimes referred to herein as a “cancer amino acid sequence”, “cancer protein”, “protein of a cancer listed in Table I”, a “cancer mRNA”, “mRNA of a cancer listed in Table I”, etc. In one embodiment, the cancer protein is encoded by a nucleic acid of  FIG. 2 . The cancer protein can be a fragment, or alternatively, be the full-length protein to the fragment encoded by the nucleic acids of  FIG. 2 . In one embodiment, a cancer amino acid sequence is used to determine sequence identity or similarity. In another embodiment, the sequences are naturally occurring allelic variants of a protein encoded by a nucleic acid of  FIG. 2 . In another embodiment, the sequences are sequence variants as further described herein. 
     “High throughput screening” assays for the presence, absence, quantification, or other properties of particular nucleic acids or protein products are well known to those of skill in the art. Similarly, binding assays and reporter gene assays are similarly well known. Thus, e.g., U.S. Pat. No. 5,559,410 discloses high throughput screening methods for proteins; U.S. Pat. No. 5,585,639 discloses high throughput screening methods for nucleic acid binding (i.e., in arrays); while U.S. Pat. Nos. 5,576,220 and 5,541,061 disclose high throughput methods of screening for ligand/antibody binding. 
     In addition, high throughput screening systems are commercially available (see, e.g., Amersham Biosciences, Piscataway, N.J.; Zymark Corp., Hopkinton, Mass.; Air Technical Industries, Mentor, Ohio; Beckman Instruments, Inc. Fullerton, Calif.; Precision Systems, Inc., Natick, Mass.; etc.). These systems typically automate entire procedures, including all sample and reagent pipetting, liquid dispensing, timed incubations, and final readings of the microplate in detector(s) appropriate for the assay. These configurable systems provide high throughput and rapid start up as well as a high degree of flexibility and customization. The manufacturers of such systems provide detailed protocols for various high throughput systems. Thus, e.g., Zymark Corp. provides technical bulletins describing screening systems for detecting the modulation of gene transcription, ligand binding, and the like. 
     The term “homolog” refers to a molecule which exhibits homology to another molecule, by for example, having sequences of chemical residues that are the same or similar at corresponding positions. 
     “Human Leukocyte Antigen” or “HLA” is a human class I or class II Major Histocompatibility Complex (MHC) protein (see, e.g., Stites, et al., Immunology, 8th Ed., Lange Publishing, Los Altos, Calif. (1994). 
     The terms “hybridize”, “hybridizing”, “hybridizes” and the like, used in the context of polynucleotides, are meant to refer to conventional hybridization conditions, preferably such as hybridization in 50% formamide/6×SSC/0.1% SDS/100 μg/ml ssDNA, in which temperatures for hybridization are above 37 degrees C. and temperatures for washing in 0.1×SSC/0.1% SDS are above 55 degrees C. 
     The phrases “isolated” or “biologically pure” refer to material which is substantially or essentially free from components which normally accompany the material as it is found in its native state. Thus, isolated peptides in accordance with the invention preferably do not contain materials normally associated with the peptides in their in situ environment. For example, a polynucleotide is said to be “isolated” when it is substantially separated from contaminant polynucleotides that correspond or are complementary to genes other than the 158P3D2 genes or that encode polypeptides other than 158P3D2 gene product or fragments thereof. A skilled artisan can readily employ nucleic acid isolation procedures to obtain an isolated 158P3D2 polynucleotide. A protein is said to be “isolated,” for example, when physical, mechanical or chemical methods are employed to remove the 158P3D2 proteins from cellular constituents that are normally associated with the protein. A skilled artisan can readily employ standard purification methods to obtain an isolated 158P3D2 protein. Alternatively, an isolated protein can be prepared by chemical means. 
     The term “mammal” refers to any organism classified as a mammal, including mice, rats, rabbits, dogs, cats, cows, horses and humans. In one embodiment of the invention, the mammal is a mouse. In another embodiment of the invention, the mammal is a human. 
     The terms “metastatic prostate cancer” and “metastatic disease” mean prostate cancers that have spread to regional lymph nodes or to distant sites, and are meant to include stage D disease under the AUA system and stage T×N×M+ under the TNM system. As is the case with locally advanced prostate cancer, surgery is generally not indicated for patients with metastatic disease, and hormonal (androgen ablation) therapy is a preferred treatment modality. Patients with metastatic prostate cancer eventually develop an androgen-refractory state within 12 to 18 months of treatment initiation. Approximately half of these androgen-refractory patients die within 6 months after developing that status. The most common site for prostate cancer metastasis is bone. Prostate cancer bone metastases are often osteoblastic rather than osteolytic (i.e., resulting in net bone formation). Bone metastases are found most frequently in the spine, followed by the femur, pelvis, rib cage, skull and humerus. Other common sites for metastasis include lymph nodes, lung, liver and brain. Metastatic prostate cancer is typically diagnosed by open or laparoscopic pelvic lymphadenectomy, whole body radionuclide scans, skeletal radiography, and/or bone lesion biopsy. 
     The term “modulator” or “test compound” or “drug candidate” or grammatical equivalents as used herein describe any molecule, e.g., protein, oligopeptide, small organic molecule, polysaccharide, polynucleotide, etc., to be tested for the capacity to directly or indirectly alter the cancer phenotype or the expression of a cancer sequence, e.g., a nucleic acid or protein sequences, or effects of cancer sequences (e.g., signaling, gene expression, protein interaction, etc.) In one aspect, a modulator will neutralize the effect of a cancer protein of the invention. By “neutralize” is meant that an activity of a protein is inhibited or blocked, along with the consequent effect on the cell. In another aspect, a modulator will neutralize the effect of a gene, and its corresponding protein, of the invention by normalizing levels of said protein. In preferred embodiments, modulators alter expression profiles, or expression profile nucleic acids or proteins provided herein, or downstream effector pathways. In one embodiment, the modulator suppresses a cancer phenotype, e.g. to a normal tissue fingerprint. In another embodiment, a modulator induced a cancer phenotype. Generally, a plurality of assay mixtures is run in parallel with different agent concentrations to obtain a differential response to the various concentrations. Typically, one of these concentrations serves as a negative control, i.e., at zero concentration or below the level of detection. 
     Modulators, drug candidates or test compounds encompass numerous chemical classes, though typically they are organic molecules, preferably small organic compounds having a molecular weight of more than 100 and less than about 2,500 Daltons. Preferred small molecules are less than 2000, or less than 1500 or less than 1000 or less than 500 D. Candidate agents comprise functional groups necessary for structural interaction with proteins, particularly hydrogen bonding, and typically include at least an amine, carbonyl, hydroxyl or carboxyl group, preferably at least two of the functional chemical groups. The candidate agents often comprise cyclical carbon or heterocyclic structures and/or aromatic or polyaromatic structures substituted with one or more of the above functional groups. Modulators also comprise biomolecules such as peptides, saccharides, fatty acids, steroids, purines, pyrimidines, derivatives, structural analogs or combinations thereof. Particularly preferred are peptides. One class of modulators are peptides, for example of from about five to about 35 amino acids, with from about five to about 20 amino acids being preferred, and from about 7 to about 15 being particularly preferred. Preferably, the cancer modulatory protein is soluble, includes a non-transmembrane region, and/or, has an N-terminal Cys to aid in solubility. In one embodiment, the C-terminus of the fragment is kept as a free acid and the N-terminus is a free amine to aid in coupling, i.e., to cysteine. In one embodiment, a cancer protein of the invention is conjugated to an immunogenic agent as discussed herein. In one embodiment, the cancer protein is conjugated to BSA. The peptides of the invention, e.g., of preferred lengths, can be linked to each other or to other amino acids to create a longer peptide/protein. The modulatory peptides can be digests of naturally occurring proteins as is outlined above, random peptides, or “biased” random peptides. In a preferred embodiment, peptide/protein-based modulators are antibodies, and fragments thereof, as defined herein. 
     Modulators of cancer can also be nucleic acids. Nucleic acid modulating agents can be naturally occurring nucleic acids, random nucleic acids, or “biased” random nucleic acids. For example, digests of prokaryotic or eukaryotic genomes can be used in an approach analogous to that outlined above for proteins. 
     The term “monoclonal antibody” refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the antibodies comprising the population are identical except for possible naturally occurring mutations that are present in minor amounts. 
     A “motif”, as in biological motif of a 158P3D2-related protein, refers to any pattern of amino acids forming part of the primary sequence of a protein, that is associated with a particular function (e.g. protein-protein interaction, protein-DNA interaction, etc) or modification (e.g. that is phosphorylated, glycosylated or amidated), or localization (e.g. secretory sequence, nuclear localization sequence, etc.) or a sequence that is correlated with being immunogenic, either humorally or cellularly. A motif can be either contiguous or capable of being aligned to certain positions that are generally correlated with a certain function or property. In the context of HLA motifs, “motif” refers to the pattern of residues in a peptide of defined length, usually a peptide of from about 8 to about 13 amino acids for a class I HLA motif and from about 6 to about 25 amino acids for a class II HLA motif, which is recognized by a particular HLA molecule. Peptide motifs for HLA binding are typically different for each protein encoded by each human HLA allele and differ in the pattern of the primary and secondary anchor residues. 
     A “pharmaceutical excipient” comprises a material such as an adjuvant, a carrier, pH-adjusting and buffering agents, tonicity adjusting agents, wetting agents, preservative, and the like. 
     “Pharmaceutically acceptable” refers to a non-toxic, inert, and/or composition that is physiologically compatible with humans or other mammals. 
     The term “polynucleotide” means a polymeric form of nucleotides of at least 10 bases or base pairs in length, either ribonucleotides or deoxynucleotides or a modified form of either type of nucleotide, and is meant to include single and double stranded forms of DNA and/or RNA. In the art, this term if often used interchangeably with “oligonucleotide”. A polynucleotide can comprise a nucleotide sequence disclosed herein wherein thymidine (T), as shown for example in  FIG. 2 , can also be uracil (U); this definition pertains to the differences between the chemical structures of DNA and RNA, in particular the observation that one of the four major bases in RNA is uracil (U) instead of thymidine (T). 
     The term “polypeptide” means a polymer of at least about 4, 5, 6, 7, or 8 amino acids. Throughout the specification, standard three letter or single letter designations for amino acids are used. In the art, this term is often used interchangeably with “peptide” or “protein”. 
     An HLA “primary anchor residue” is an amino acid at a specific position along a peptide sequence which is understood to provide a contact point between the immunogenic peptide and the HLA molecule. One to three, usually two, primary anchor residues within a peptide of defined length generally defines a “motif” for an immunogenic peptide. These residues are understood to fit in close contact with peptide binding groove of an HLA molecule, with their side chains buried in specific pockets of the binding groove. In one embodiment, for example, the primary anchor residues for an HLA class I molecule are located at position 2 (from the amino terminal position) and at the carboxyl terminal position of a 8, 9, 10, 11, or 12 residue peptide epitope in accordance with the invention. Alternatively, in another embodiment, the primary anchor residues of a peptide binds an HLA class II molecule are spaced relative to each other, rather than to the termini of a peptide, where the peptide is generally of at least 9 amino acids in length. The primary anchor positions for each motif and supermotif are set forth in Table IV. For example, analog peptides can be created by altering the presence or absence of particular residues in the primary and/or secondary anchor positions shown in Table IV. Such analogs are used to modulate the binding affinity and/or population coverage of a peptide comprising a particular HLA motif or supermotif. 
     “Radioisotopes” include, but are not limited to the following (non-limiting exemplary uses are also set forth): 
     Examples of Medical Isotopes 
     
       
         
           
               
               
             
               
                   
               
               
                 Isotope 
                 Description of use 
               
               
                   
               
             
            
               
                 Actinium-225 
                 See Thorium-229 (Th-229) 
               
               
                 (AC-225) 
                   
               
               
                 Actinium-227 
                 Parent of Radium-223 (Ra-223) which is an alpha emitter used to 
               
               
                 (AC-227) 
                 treat metastases in the skeleton resulting from cancer (i.e., 
               
               
                   
                 breast and prostate cancers), and cancer radioimmunotherapy 
               
               
                 Bismuth-212 
                 See Thorium-228 (Th-228) 
               
               
                 (Bi-212) 
                   
               
               
                 Bismuth-213 
                 See Thorium-229 (Th-229) 
               
               
                 (Bi-213) 
                   
               
               
                 Cadmium-109 
                 Cancer detection 
               
               
                 (Cd-109) 
                   
               
               
                 Cobalt-60 
                 Radiation source for radiotherapy of cancer, for food irradiators, and 
               
               
                 (Co-60) 
                 for sterilization of medical supplies 
               
               
                 Copper-64 
                 A positron emitter used for cancer therapy and SPECT imaging 
               
               
                 (Cu-64) 
                   
               
               
                 Copper-67 
                 Beta/gamma emitter used in cancer radioimmunotherapy and 
               
               
                 (Cu-67) 
                 diagnostic studies (i.e., breast and colon cancers, and lymphoma) 
               
               
                 Dysprosium-166 
                 Cancer radioimmunotherapy 
               
               
                 (Dy-166) 
                   
               
               
                 Erbium-169 
                 Rheumatoid arthritis treatment, particularly for the small joints 
               
               
                 (Er-169) 
                 associated with fingers and toes 
               
               
                 Europium-152 
                 Radiation source for food irradiation and for sterilization of medical 
               
               
                 (Eu-152) 
                 supplies 
               
               
                 Europium-154 
                 Radiation source for food irradiation and for sterilization of medical 
               
               
                 (Eu-154) 
                 supplies 
               
               
                 Gadolinium-153 
                 Osteoporosis detection and nuclear medical quality assurance devices 
               
               
                 (Gd-153) 
                   
               
               
                 Gold-198 
                 Implant and intracavity therapy of ovarian, prostate, and brain 
               
               
                 (Au-198) 
                 cancers 
               
               
                 Holmium-166 
                 Multiple myeloma treatment in targeted skeletal therapy, cancer 
               
               
                 (Ho-166) 
                 radioimmunotherapy, bone marrow ablation, and rheumatoid arthritis 
               
               
                   
                 treatment 
               
               
                 Iodine-125 
                 Osteoporosis detection, diagnostic imaging, tracer drugs, brain cancer 
               
               
                 (I-125) 
                 treatment, radiolabeling, tumor imaging, mapping of receptors in the 
               
               
                   
                 brain, interstitial radiation therapy, brachytherapy for treatment of 
               
               
                   
                 prostate cancer, determination of glomerular filtration rate (GFR), 
               
               
                   
                 determination of plasma volume, detection of deep vein thrombosis 
               
               
                   
                 of the legs 
               
               
                 Iodine-131 
                 Thyroid function evaluation, thyroid disease detection, treatment of 
               
               
                 (I-131) 
                 thyroid cancer as well as other non-malignant thyroid diseases (i.e., 
               
               
                   
                 Graves disease, goiters, and hyperthyroidism), treatment of leukemia, 
               
               
                   
                 lymphoma, and other forms of cancer (e.g., breast cancer) using 
               
               
                   
                 radioimmunotherapy 
               
               
                 Iridium-192 
                 Brachytherapy, brain and spinal cord tumor treatment, treatment of 
               
               
                 (Ir-192) 
                 blocked arteries (i.e., arteriosclerosis and restenosis), and implants for 
               
               
                   
                 breast and prostate tumors 
               
               
                 Lutetium-177 
                 Cancer radioimmunotherapy and treatment of blocked arteries (i.e., 
               
               
                 (Lu-177) 
                 arteriosclerosis and restenosis) 
               
               
                 Molybdenum-99 
                 Parent of Technetium-99m (Tc-99m) which is used for imaging the 
               
               
                 (Mo-99) 
                 brain, liver, lungs, heart, and other organs. Currently, Tc-99m is the 
               
               
                   
                 most widely used radioisotope used for diagnostic imaging of various 
               
               
                   
                 cancers and diseases involving the brain, heart, liver, lungs; also used 
               
               
                   
                 in detection of deep vein thrombosis of the legs 
               
               
                 Osmium-194 
                 Cancer radioimmunotherapy 
               
               
                 (Os-194) 
                   
               
               
                 Palladium-103 
                 Prostate cancer treatment 
               
               
                 (Pd-103) 
                   
               
               
                 Platinum-195m 
                 Studies on biodistribution and metabolism of cisplatin, a 
               
               
                 (Pt-195m) 
                 chemotherapeutic drug 
               
               
                 Phosphorus-32 
                 Polycythemia rubra vera (blood cell disease) and leukemia treatment, 
               
               
                 (P-32) 
                 bone cancer diagnosis/treatment; colon, pancreatic, and liver cancer 
               
               
                   
                 treatment; radiolabeling nucleic acids for in vitro research, diagnosis 
               
               
                   
                 of superficial tumors, treatment of blocked arteries (i.e., 
               
               
                   
                 arteriosclerosis and restenosis), and intracavity therapy 
               
               
                 Phosphorus-33 
                 Leukemia treatment, bone disease diagnosis/treatment, radiolabeling, 
               
               
                 (P-33) 
                 and treatment of blocked arteries (i.e., arteriosclerosis and restenosis) 
               
               
                 Radium-223 
                 See Actinium-227 (Ac-227) 
               
               
                 (Ra-223) 
                   
               
               
                 Rhenium-186 
                 Bone cancer pain relief, rheumatoid arthritis treatment, and diagnosis 
               
               
                 (Re-186) 
                 and treatment of lymphoma and bone, breast, colon, and liver cancers 
               
               
                   
                 using radioimmunotherapy 
               
               
                 Rhenium-188 
                 Cancer diagnosis and treatment using radioimmunotherapy, bone 
               
               
                 (Re-188) 
                 cancer pain relief, treatment of rheumatoid arthritis, and treatment of 
               
               
                   
                 prostate cancer 
               
               
                 Rhodium-105 
                 Cancer radioimmunotherapy 
               
               
                 (Rh-105) 
                   
               
               
                 Samarium-145 
                 Ocular cancer treatment 
               
               
                 (Sm-145) 
                   
               
               
                 Samarium-153 
                 Cancer radioimmunotherapy and bone cancer pain relief 
               
               
                 (Sm-153) 
                   
               
               
                 Scandium-47 
                 Cancer radioimmunotherapy and bone cancer pain relief 
               
               
                 (Sc-47) 
                   
               
               
                 Selenium-75 
                 Radiotracer used in brain studies, imaging of adrenal cortex 
               
               
                 (Se-75) 
                 by gamma-scintigraphy, lateral locations of steroid secreting tumors, 
               
               
                   
                 pancreatic scanning, detection of hyperactive parathyroid glands, 
               
               
                   
                 measure rate of bile acid loss from the endogenous pool 
               
               
                 Strontium-85 
                 Bone cancer detection and brain scans 
               
               
                 (Sr-85) 
                   
               
               
                 Strontium-89 
                 Bone cancer pain relief, multiple myeloma treatment, and osteoblastic 
               
               
                 (Sr-89) 
                 therapy 
               
               
                 Technetium-99m 
                 See Molybdenum-99 (Mo-99) 
               
               
                 (Tc-99m) 
                   
               
               
                 Thorium-228 
                 Parent of Bismuth-212 (Bi-212) which is an alpha emitter used in 
               
               
                 (Th-228) 
                 cancer radioimmunotherapy 
               
               
                 Thorium-229 
                 Parent of Actinium-225 (Ac-225) and grandparent of Bismuth-213 
               
               
                 (Th-229) 
                 (Bi-213) which are alpha emitters used in cancer 
               
               
                   
                 radioimmunotherapy 
               
               
                 Thulium-170 
                 Gamma source for blood irradiators, energy source for implanted 
               
               
                 (Tm-170) 
                 medical devices 
               
               
                 Tin-117m 
                 Cancer immunotherapy and bone cancer pain relief 
               
               
                 (Sn-117m) 
                   
               
               
                 Tungsten-188 
                 Parent for Rhenium-188 (Re-188) which is used for cancer 
               
               
                 (W-188) 
                 diagnostics/treatment, bone cancer pain relief, rheumatoid arthritis 
               
               
                   
                 treatment, and treatment of blocked arteries (i.e., arteriosclerosis and 
               
               
                   
                 restenosis) 
               
               
                 Xenon-127 
                 Neuroimaging of brain disorders, high resolution SPECT studies, 
               
               
                 (Xe-127) 
                 pulmonary function tests, and cerebral blood flow studies 
               
               
                 Ytterbium-175 
                 Cancer radioimmunotherapy 
               
               
                 (Yb-175) 
                   
               
               
                 Yttrium-90 
                 Microseeds obtained from irradiating Yttrium-89 (Y-89) for liver 
               
               
                 (Y-90) 
                 cancer treatment 
               
               
                 Yttrium-91 
                 A gamma-emitting label for Yttrium-90 (Y-90) which is used for 
               
               
                 (Y-91) 
                 cancer radioimmunotherapy (i.e., lymphoma, breast, colon, kidney, 
               
               
                   
                 lung, ovarian, prostate, pancreatic, and inoperable liver cancers) 
               
               
                   
               
            
           
         
       
     
     By “randomized” or grammatical equivalents as herein applied to nucleic acids and proteins is meant that each nucleic acid and peptide consists of essentially random nucleotides and amino acids, respectively. These random peptides (or nucleic acids, discussed herein) can incorporate any nucleotide or amino acid at any position. The synthetic process can be designed to generate randomized proteins or nucleic acids, to allow the formation of all or most of the possible combinations over the length of the sequence, thus forming a library of randomized candidate bioactive proteinaceous agents. 
     In one embodiment, a library is “fully randomized,” with no sequence preferences or constants at any position. In another embodiment, the library is a “biased random” library. That is, some positions within the sequence either are held constant, or are selected from a limited number of possibilities. For example, the nucleotides or amino acid residues are randomized within a defined class, e.g., of hydrophobic amino acids, hydrophilic residues, sterically biased (either small or large) residues, towards the creation of nucleic acid binding domains, the creation of cysteines, for cross-linking, prolines for SH-3 domains, serines, threonines, tyrosines or histidines for phosphorylation sites, etc., or to purines, etc. 
     A “recombinant” DNA or RNA molecule is a DNA or RNA molecule that has been subjected to molecular manipulation in vitro. 
     Non-limiting examples of small molecules include compounds that bind or interact with 158P3D2, ligands including hormones, neuropeptides, chemokines, odorants, phospholipids, and functional equivalents thereof that bind and preferably inhibit 158P3D2 protein function. Such non-limiting small molecules preferably have a molecular weight of less than about 10 kDa, more preferably below about 9, about 8, about 7, about 6, about 5 or about 4 kDa. In certain embodiments, small molecules physically associate with, or bind, 158P3D2 protein; are not found in naturally occurring metabolic pathways; and/or are more soluble in aqueous than non-aqueous solutions 
     “Stringency” of hybridization reactions is readily determinable by one of ordinary skill in the art, and generally is an empirical calculation dependent upon probe length, washing temperature, and salt concentration. In general, longer probes require higher temperatures for proper annealing, while shorter probes need lower temperatures. Hybridization generally depends on the ability of denatured nucleic acid sequences to reanneal when complementary strands are present in an environment below their melting temperature. The higher the degree of desired homology between the probe and hybridizable sequence, the higher the relative temperature that can be used. As a result, it follows that higher relative temperatures would tend to make the reaction conditions more stringent, while lower temperatures less so. For additional details and explanation of stringency of hybridization reactions, see Ausubel et al., Current Protocols in Molecular Biology, Wiley Interscience Publishers, (1995). 
     “Stringent conditions” or “high stringency conditions”, as defined herein, are identified by, but not limited to, those that: (1) employ low ionic strength and high temperature for washing, for example 0.015 M sodium chloride/0.0015 M sodium citrate/0.1% sodium dodecyl sulfate at 50° C.; (2) employ during hybridization a denaturing agent, such as formamide, for example, 50% (v/v) formamide with 0.1% bovine serum albumin/0.1% Ficoll/0.1% polyvinylpyrrolidone/50 mM sodium phosphate buffer at pH 6.5 with 750 mM sodium chloride, 75 mM sodium citrate at 42° C.; or (3) employ 50% formamide, 5×SSC (0.75 M NaCl, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5×Denhardt&#39;s solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC (sodium chloride/sodium. citrate) and 50% formamide at 55° C., followed by a high-stringency wash consisting of 0.1×SSC containing EDTA at 55° C. “Moderately stringent conditions” are described by, but not limited to, those in Sambrook et al., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Press, 1989, and include the use of washing solution and hybridization conditions (e.g., temperature, ionic strength and % SDS) less stringent than those described above. An example of moderately stringent conditions is overnight incubation at 37° C. in a solution comprising: 20% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt&#39;s solution, 10% dextran sulfate, and 20 mg/mL denatured sheared salmon sperm DNA, followed by washing the filters in 1×SSC at about 37-50° C. The skilled artisan will recognize how to adjust the temperature, ionic strength, etc. as necessary to accommodate factors such as probe length and the like. 
     An HLA “supermotif” is a peptide binding specificity shared by HLA molecules encoded by two or more HLA alleles. Overall phenotypic frequencies of HLA-supertypes in different ethnic populations are set forth in Table IV (F). The non-limiting constituents of various supertypes are as follows:
         A2: A*0201, A*0202, A*0203, A*0204, A*0205, A*0206, A*6802, A*6901, A*0207   A3: A3, All, A31, A*3301, A*6801, A*0301, A*1101, A*3101   B7: B7, B*3501-03, B*51, B*5301, B*5401, B*5501, B*5502, B*5601, B*6701, B*7801, B*0702, B*5101, B*5602   B44: B*3701, B*4402, B*4403, B*60 (B*4001), B61 (B*4006)   A1: A*0102, A*2604, A*3601, A*4301, A*8001   A24: A*24, A*30, A*2403, A*2404, A*3002, A*3003   B27: B*1401-02, B*1503, B*1509, B*1510, B*1518, B*3801-02, B*3901, B*3902, B*3903-04, B*4801-02, B*7301, B*2701-08   B58: B*1516, B*1517, B*5701, B*5702, B58   B62: B*4601, B52, B*1501 (B62), B*1502 (B75), B*1513 (B77)       

     Calculated population coverage afforded by different HLA-supertype combinations are set forth in Table IV (G). 
     As used herein “to treat” or “therapeutic” and grammatically related terms, refer to any improvement of any consequence of disease, such as prolonged survival, less morbidity, and/or a lessening of side effects which are the byproducts of an alternative therapeutic modality; full eradication of disease is not required. 
     A “transgenic animal” (e.g., a mouse or rat) is an animal having cells that contain a transgene, which transgene was introduced into the animal or an ancestor of the animal at a prenatal, e.g., an embryonic stage. A “transgene” is a DNA that is integrated into the genome of a cell from which a transgenic animal develops. 
     As used herein, an HLA or cellular immune response “vaccine” is a composition that contains or encodes one or more peptides of the invention. There are numerous embodiments of such vaccines, such as a cocktail of one or more individual peptides; one or more peptides of the invention comprised by a polyepitopic peptide; or nucleic acids that encode such individual peptides or polypeptides, e.g., a minigene that encodes a polyepitopic peptide. The “one or more peptides” can include any whole unit integer from 1-150 or more, e.g., at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, or 150 or more peptides of the invention. The peptides or polypeptides can optionally be modified, such as by lipidation, addition of targeting or other sequences. HLA class I peptides of the invention can be admixed with, or linked to, HLA class II peptides, to facilitate activation of both cytotoxic T lymphocytes and helper T lymphocytes. HLA vaccines can also comprise peptide-pulsed antigen presenting cells, e.g., dendritic cells. 
     The term “variant” refers to a molecule that exhibits a variation from a described type or norm, such as a protein that has one or more different amino acid residues in the corresponding position(s) of a specifically described protein (e.g. the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 . An analog is an example of a variant protein. Splice isoforms and single nucleotides polymorphisms (SNPs) are further examples of variants. 
     The “158P3D2-related proteins” of the invention include those specifically identified herein, as well as allelic variants, conservative substitution variants, analogs and homologs that can be isolated/generated and characterized without undue experimentation following the methods outlined herein or readily available in the art. Fusion proteins that combine parts of different 158P3D2 proteins or fragments thereof, as well as fusion proteins of a 158P3D2 protein and a heterologous polypeptide are also included. Such 158P3D2 proteins are collectively referred to as the 158P3D2-related proteins, the proteins of the invention, or 158P3D2. The term “158P3D2-related protein” refers to a polypeptide fragment or a 158P3D2 protein sequence of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, or more than 25 amino acids; or, at least 30, 35, 40, 45, 50, 55, 60, 65, 70, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 525, 550, 575, or 576 or more amino acids. 
     II.) 158P3D2 Polynucleotides 
     One aspect of the invention provides polynucleotides corresponding or complementary to all or part of a 158P3D2 gene, mRNA, and/or coding sequence, preferably in isolated form, including polynucleotides encoding a 158P3D2-related protein and fragments thereof, DNA, RNA, DNA/RNA hybrid, and related molecules, polynucleotides or oligonucleotides complementary to a 158P3D2 gene or mRNA sequence or a part thereof, and polynucleotides or oligonucleotides that hybridize to a 158P3D2 gene, mRNA, or to a 158P3D2 encoding polynucleotide (collectively, “158P3D2 polynucleotides”). In all instances when referred to in this section, T can also be U in  FIG. 2 . 
     Embodiments of a 158P3D2 polynucleotide include: a 158P3D2 polynucleotide having the sequence shown in  FIG. 2 , the nucleotide sequence of 158P3D2 as shown in  FIG. 2  wherein T is U; at least 10 contiguous nucleotides of a polynucleotide having the sequence as shown in  FIG. 2 ; or, at least 10 contiguous nucleotides of a polynucleotide having the sequence as shown in  FIG. 2  where T is U. For example, embodiments of 158P3D2 nucleotides comprise, without limitation: 
     (I) a polynucleotide comprising, consisting essentially of, or consisting of a sequence as shown in  FIG. 2 , wherein T can also be U; 
     (II) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2A , from nucleotide residue number 849 through nucleotide residue number 1835, including the stop codon, wherein T can also be U; 
     (III) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2B , from nucleotide residue number 117 through nucleotide residue number 827, including the stop codon, wherein T can also be U; 
     (IV) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2C , from nucleotide residue number 2249 through nucleotide residue number 2794, including the a stop codon, wherein T can also be U; 
     (V) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2D , from nucleotide residue number 849 through nucleotide residue number 1835, including the stop codon, wherein T can also be U; 
     (VI) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2E , from nucleotide residue number 849 through nucleotide residue number 1835, including the stop codon, wherein T can also be U; 
     (VII) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2F , from nucleotide residue number 849 through nucleotide residue number 1835, including the stop codon, wherein T can also be U; 
     (VIII) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2G , from nucleotide residue number 1289 through nucleotide residue number 1834, including the stop codon, wherein T can also be U; 
     (IX) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2H , from nucleotide residue number 849 through nucleotide residue number 1835, including the stop codon, wherein T can also be U; 
     (X) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2I , from nucleotide residue number 849 through nucleotide residue number 1835, including the stop codon, wherein T can also be U; 
     (XI) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2J , from nucleotide residue number 849 through nucleotide residue number 1835, including the stop codon, wherein T can also be U; 
     (XII) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2K , from nucleotide residue number 65 through nucleotide residue number 4246, including the stop codon, wherein T can also be U; 
     (XIII) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2L , from nucleotide residue number 65 through nucleotide residue number 3502, including the stop codon, wherein T can also be U; 
     (XIV) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2M , from nucleotide residue number 65 through nucleotide residue number 6037, including the stop codon, wherein T can also be U; 
     (XV) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2N , from nucleotide residue number 65 through nucleotide residue number 6175, including the stop codon, wherein T can also be U; 
     (XVI) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2O , from nucleotide residue number 2932 through nucleotide residue number 4764, including the stop codon, wherein T can also be U; 
     (XVII) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2P , from nucleotide residue number 65 through nucleotide residue number 6001, including the stop codon, wherein T can also be U; 
     (XVIII) a polynucleotide comprising, consisting essentially of, or consisting of the sequence as shown in  FIG. 2Q , from nucleotide residue number 65 through nucleotide residue number 6121, including the stop codon, wherein T can also be U; 
     (XIX) a polynucleotide that encodes a 158P3D2-related protein that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% homologous to an entire amino acid sequence shown in  FIG. 2A-Q ; 
     (XX) a polynucleotide that encodes a 158P3D2-related protein that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identical to an entire amino acid sequence shown in  FIG. 2A-Q ; 
     (XXI) a polynucleotide that encodes at least one peptide set forth in Tables VIII-XXI and XXII-XLIX; 
     (XXII) a polynucleotide that encodes a peptide region of at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a peptide of  FIGS. 3A-3R  in any whole number increment up to 328, 236, 181, 178, 181, 1393, 1145, 1990, 2036, 610, 1978, and 2018 that includes at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value greater than 0.5 in the Hydrophilicity profile of  FIG. 5 ; 
     (XXIII) a polynucleotide that encodes a peptide region of at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a peptide of  FIGS. 3A-3R  in any whole number increment up to 328, 236, 181, 178, 181, 1393, 1145, 1990, 2036, 610, 1978, and 2018 that includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value less than 0.5 in the Hydropathicity profile of  FIG. 6 ; 
     (XXIV) a polynucleotide that encodes a peptide region of at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a peptide of  FIGS. 3A-3R  in any whole number increment up to 328, 236, 181, 178, 181, 1393, 1145, 1990, 2036, 610, 1978, and 2018 that includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value greater than 0.5 in the Percent Accessible Residues profile of  FIG. 7 ; 
     (XXV) a polynucleotide that encodes a peptide region of at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a peptide of  FIG. 3A-3R  in any whole number increment up to 328, 236, 181, 178, 181, 1393, 1145, 1990, 2036, 610, 1978, and 2018 that includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value greater than 0.5 in the Average Flexibility profile of  FIG. 8 ; 
     (XXVI) a polynucleotide that encodes a peptide region of at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a peptide of  FIG. 3A-3R  in any whole number increment up to 328, 236, 181, 178, 181, 1393, 1145, 1990, 2036, 610, 1978, and 2018 that includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value greater than 0.5 in the Beta-turn profile of  FIG. 9 ; 
     (XXVII) a polynucleotide that is fully complementary to a polynucleotide of any one of (I)-(XXVI); 
     (XXVIII) a polynucleotide that is fully complementary to a polynucleotide of any one of (I)-(XXVII); 
     (XXIX) a peptide that is encoded by any of (I) to (XXVIII); and; 
     (XXX) a composition comprising a polynucleotide of any of (I)-(XXVIII) or peptide of (XXIX) together with a pharmaceutical excipient and/or in a human unit dose form; 
     (XXXI) a method of using a polynucleotide of any (I)-(XXVIII) or peptide of (XXIX) or a composition of (XXX) in a method to modulate a cell expressing 158P3D2; 
     (XXXII) a method of using a polynucleotide of any (I)-(XXVIII) or peptide of (XXIX) or a composition of (XXX) in a method to diagnose, prophylax, prognose, or treat an individual who bears a cell expressing 158P3D2; 
     (XXIII) a method of using a polynucleotide of any (I)-(XXVIII) or peptide of (XXIX) or a composition of (XXX) in a method to diagnose, prophylax, prognose, or treat an individual who bears a cell expressing 158P3D2, said cell from a cancer of a tissue listed in Table I; 
     (XXXIV) a method of using a polynucleotide of any (I)-(XXVIII) or peptide of (XXIX) or a composition of (XXX) in a method to diagnose, prophylax, prognose, or treat a cancer; 
     (XXXV) a method of using a polynucleotide of any (I)-(XXVIII) or peptide of (XXIX) or a composition of (XXX) in a method to diagnose, prophylax, prognose, or treat a cancer of a tissue listed in Table I; and; 
     (XXXVI) a method of using a polynucleotide of any (I)-(XXVIII) or peptide of (XXIX) or a composition of (XXX) in a method to identify or characterize a modulator of a cell expressing 158P3D2. 
     As used herein, a range is understood to disclose specifically all whole unit positions thereof. 
     Typical embodiments of the invention disclosed herein include 158P3D2 polynucleotides that encode specific portions of 158P3D2 mRNA sequences (and those which are complementary to such sequences) such as those that encode the proteins and/or fragments thereof, for example: 
     (a) 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 225, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310, 315, 320, 325 and 328 or more contiguous amino acids of 158P3D2 variant 1; the maximal lengths relevant for other variants are shown in  FIGS. 2A-2Q  and  3 A- 3 R respectively. 
     For example, representative embodiments of the invention disclosed herein include: polynucleotides and their encoded peptides themselves encoding about amino acid 1 to about amino acid 10 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 10 to about amino acid 20 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 20 to about amino acid 30 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 30 to about amino acid 40 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 40 to about amino acid 50 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 50 to about amino acid 60 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 60 to about amino acid 70 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 70 to about amino acid 80 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 80 to about amino acid 90 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polynucleotides encoding about amino acid 90 to about amino acid 100 of the 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , in increments of about 10 amino acids, ending at the carboxyl terminal amino acid set forth in  FIG. 2  or  FIG. 3 . Accordingly, polynucleotides encoding portions of the amino acid sequence (of about 10 amino acids), of amino acids, 100 through the carboxyl terminal amino acid of the 158P3D2 protein are embodiments of the invention. Wherein it is understood that each particular amino acid position discloses that position plus or minus five amino acid residues. 
     Polynucleotides encoding relatively long portions of a 158P3D2 protein are also within the scope of the invention. For example, polynucleotides encoding from about amino acid 1 (or 20 or 30 or 40 etc.) to about amino acid 20, (or 30, or 40 or 50 etc.) of the 158P3D2 protein “or variant” shown in  FIG. 2  or  FIG. 3  can be generated by a variety of techniques well known in the art. These polynucleotide fragments can include any portion of the 158P3D2 sequence as shown in  FIG. 2 . 
     Additional illustrative embodiments of the invention disclosed herein include 158P3D2 polynucleotide fragments encoding one or more of the biological motifs contained within a 158P3D2 protein “or variant” sequence, including one or more of the motif-bearing subsequences of a 158P3D2 protein “or variant” set forth in Tables VIII-XXI and XXII-XLIX. In another embodiment, typical polynucleotide fragments of the invention encode one or more of the regions of 158P3D2 protein or variant that exhibit homology to a known molecule. In another embodiment of the invention, typical polynucleotide fragments can encode one or more of the 158P3D2 protein or variant N-glycosylation sites, cAMP and cGMP-dependent protein kinase phosphorylation sites, casein kinase II phosphorylation sites or N-myristoylation site and amidation sites. 
     Note that to determine the starting position of any peptide set forth in Tables VIII-XXI and Tables XXII to XLIX (collectively HLA Peptide Tables) respective to its parental protein, e.g., variant 1, variant 2, etc., reference is made to three factors: the particular variant, the length of the peptide in an HLA Peptide Table, and the Search Peptides listed in Table VII. Generally, a unique Search Peptide is used to obtain HLA peptides for a particular variant. The position of each Search Peptide relative to its respective parent molecule is listed in Table VII. Accordingly, if a Search Peptide begins at position “X”, one must add the value “X minus 1” to each position in Tables VIII-XXI and Tables XXII-IL to obtain the actual position of the HLA peptides in their parental molecule. For example if a particular Search Peptide begins at position 150 of its parental molecule, one must add 150−1, i.e., 149 to each HLA peptide amino acid position to calculate the position of that amino acid in the parent molecule. 
     II.A.) Uses of 158P3D2 Polynucleotides 
     II.A.1. Monitoring of Genetic Abnormalities 
     The polynucleotides of the preceding paragraphs have a number of different specific uses. The human 158P3D2 gene maps to the chromosomal location set forth in the Example entitled “Chromosomal Mapping of 158P3D2.” For example, because the 158P3D2 gene maps to this chromosome, polynucleotides that encode different regions of the 158P3D2 proteins are used to characterize cytogenetic abnormalities of this chromosomal locale, such as abnormalities that are identified as being associated with various cancers. In certain genes, a variety of chromosomal abnormalities including rearrangements have been identified as frequent cytogenetic abnormalities in a number of different cancers (see e.g. Krajinovic et al., Mutat. Res. 382(3-4): 81-83 (1998); Johansson et al., Blood 86(10): 3905-3914 (1995) and Finger et al., P.N.A.S. 85(23): 9158-9162 (1988)). Thus, polynucleotides encoding specific regions of the 158P3D2 proteins provide new tools that can be used to delineate, with greater precision than previously possible, cytogenetic abnormalities in the chromosomal region that encodes 158P3D2 that may contribute to the malignant phenotype. In this context, these polynucleotides satisfy a need in the art for expanding the sensitivity of chromosomal screening in order to identify more subtle and less common chromosomal abnormalities (see e.g. Evans et al., Am. J. Obstet. Gynecol 171(4): 1055-1057 (1994)). 
     Furthermore, as 158P3D2 was shown to be highly expressed in prostate and other cancers, 158P3D2 polynucleotides are used in methods assessing the status of 158P3D2 gene products in normal versus cancerous tissues. Typically, polynucleotides that encode specific regions of the 158P3D2 proteins are used to assess the presence of perturbations (such as deletions, insertions, point mutations, or alterations resulting in a loss of an antigen etc.) in specific regions of the 158P3D2 gene, such as regions containing one or more motifs. Exemplary assays include both RT-PCR assays as well as single-strand conformation polymorphism (SSCP) analysis (see, e.g., Marrogi et al., J. Cutan. Pathol. 26(8): 369-378 (1999), both of which utilize polynucleotides encoding specific regions of a protein to examine these regions within the protein. 
     II.A.2. Antisense Embodiments 
     Other specifically contemplated nucleic acid related embodiments of the invention disclosed herein are genomic DNA, cDNAs, ribozymes, and antisense molecules, as well as nucleic acid molecules based on an alternative backbone, or including alternative bases, whether derived from natural sources or synthesized, and include molecules capable of inhibiting the RNA or protein expression of 158P3D2. For example, antisense molecules can be RNAs or other molecules, including peptide nucleic acids (PNAs) or non-nucleic acid molecules such as phosphorothioate derivatives that specifically bind DNA or RNA in a base pair-dependent manner. A skilled artisan can readily obtain these classes of nucleic acid molecules using the 158P3D2 polynucleotides and polynucleotide sequences disclosed herein. 
     Antisense technology entails the administration of exogenous oligonucleotides that bind to a target polynucleotide located within the cells. The term “antisense” refers to the fact that such oligonucleotides are complementary to their intracellular targets, e.g., 158P3D2. See for example, Jack Cohen, Oligodeoxynucleotides, Antisense Inhibitors of Gene Expression, CRC Press, 1989; and Synthesis 1:1-5 (1988). The 158P3D2 antisense oligonucleotides of the present invention include derivatives such as S-oligonucleotides (phosphorothioate derivatives or S-oligos, see, Jack Cohen, supra), which exhibit enhanced cancer cell growth inhibitory action. S-oligos (nucleoside phosphorothioates) are isoelectronic analogs of an oligonucleotide (O-oligo) in which a nonbridging oxygen atom of the phosphate group is replaced by a sulfur atom. The S-oligos of the present invention can be prepared by treatment of the corresponding O-oligos with 3H-1,2-benzodithiol-3-one-1,1-dioxide, which is a sulfur transfer reagent. See, e.g., Iyer, R. P. et al., J. Org. Chem. 55:4693-4698 (1990); and Iyer, R. P. et al., J. Am. Chem. Soc. 112:1253-1254 (1990). Additional 158P3D2 antisense oligonucleotides of the present invention include morpholino antisense oligonucleotides known in the art (see, e.g., Partridge et al., 1996, Antisense &amp; Nucleic Acid Drug Development 6: 169-175). 
     The 158P3D2 antisense oligonucleotides of the present invention typically can be RNA or DNA that is complementary to and stably hybridizes with the first 100 5′ codons or last 100 3′ codons of a 158P3D2 genomic sequence or the corresponding mRNA. Absolute complementarity is not required, although high degrees of complementarity are preferred. Use of an oligonucleotide complementary to this region allows for the selective hybridization to 158P3D2 mRNA and not to mRNA specifying other regulatory subunits of protein kinase. In one embodiment, 158P3D2 antisense oligonucleotides of the present invention are 15 to 30-mer fragments of the antisense DNA molecule that have a sequence that hybridizes to 158P3D2 mRNA. Optionally, 158P3D2 antisense oligonucleotide is a 30-mer oligonucleotide that is complementary to a region in the first 10 5′ codons or last 10 3′ codons of 158P3D2. Alternatively, the antisense molecules are modified to employ ribozymes in the inhibition of 158P3D2 expression, see, e.g., L. A. Couture &amp; D. T. Stinchcomb; Trends Genet. 12: 510-515 (1996). 
     II.A.3. Primers and Primer Pairs 
     Further specific embodiments of these nucleotides of the invention include primers and primer pairs, which allow the specific amplification of polynucleotides of the invention or of any specific parts thereof, and probes that selectively or specifically hybridize to nucleic acid molecules of the invention or to any part thereof. Probes can be labeled with a detectable marker, such as, for example, a radioisotope, fluorescent compound, bioluminescent compound, a chemiluminescent compound, metal chelator or enzyme. Such probes and primers are used to detect the presence of a 158P3D2 polynucleotide in a sample and as a means for detecting a cell expressing a 158P3D2 protein. 
     Examples of such probes include polypeptides comprising all or part of the human 158P3D2 cDNA sequence shown in  FIG. 2 . Examples of primer pairs capable of specifically amplifying 158P3D2 mRNAs are also described in the Examples. As will be understood by the skilled artisan, a great many different primers and probes can be prepared based on the sequences provided herein and used effectively to amplify and/or detect a 158P3D2 mRNA. 
     The 158P3D2 polynucleotides of the invention are useful for a variety of purposes, including but not limited to their use as probes and primers for the amplification and/or detection of the 158P3D2 gene(s), mRNA(s), or fragments thereof; as reagents for the diagnosis and/or prognosis of prostate cancer and other cancers; as coding sequences capable of directing the expression of 158P3D2 polypeptides; as tools for modulating or inhibiting the expression of the 158P3D2 gene(s) and/or translation of the 158P3D2 transcript(s); and as therapeutic agents. 
     The present invention includes the use of any probe as described herein to identify and isolate a 158P3D2 or 158P3D2 related nucleic acid sequence from a naturally occurring source, such as humans or other mammals, as well as the isolated nucleic acid sequence per se, which would comprise all or most of the sequences found in the probe used. 
     II.A.4. Isolation of 158P3D2-Encoding Nucleic Acid Molecules 
     The 158P3D2 cDNA sequences described herein enable the isolation of other polynucleotides encoding 158P3D2 gene product(s), as well as the isolation of polynucleotides encoding 158P3D2 gene product homologs, alternatively spliced isoforms, allelic variants, and mutant forms of a 158P3D2 gene product as well as polynucleotides that encode analogs of 158P3D2-related proteins. Various molecular cloning methods that can be employed to isolate full length cDNAs encoding a 158P3D2 gene are well known (see, for example, Sambrook, J. et al., Molecular Cloning: A Laboratory Manual, 2d edition, Cold Spring Harbor Press, New York, 1989; Current Protocols in Molecular Biology. Ausubel et al., Eds., Wiley and Sons, 1995). For example, lambda phage cloning methodologies can be conveniently employed, using commercially available cloning systems (e.g., Lambda ZAP Express, Stratagene). Phage clones containing 158P3D2 gene cDNAs can be identified by probing with a labeled 158P3D2 cDNA or a fragment thereof. For example, in one embodiment, a 158P3D2 cDNA (e.g.,  FIG. 2 ) or a portion thereof can be synthesized and used as a probe to retrieve overlapping and full-length cDNAs corresponding to a 158P3D2 gene. A 158P3D2 gene itself can be isolated by screening genomic DNA libraries, bacterial artificial chromosome libraries (BACs), yeast artificial chromosome libraries (YACs), and the like, with 158P3D2 DNA probes or primers. 
     II.A.5. Recombinant Nucleic Acid Molecules and Host-Vector Systems 
     The invention also provides recombinant DNA or RNA molecules containing a 158P3D2 polynucleotide, a fragment, analog or homologue thereof, including but not limited to phages, plasmids, phagemids, cosmids, YACs, BACs, as well as various viral and non-viral vectors well known in the art, and cells transformed or transfected with such recombinant DNA or RNA molecules. Methods for generating such molecules are well known (see, for example, Sambrook et al., 1989, supra). 
     The invention further provides a host-vector system comprising a recombinant DNA molecule containing a 158P3D2 polynucleotide, fragment, analog or homologue thereof within a suitable prokaryotic or eukaryotic host cell. Examples of suitable eukaryotic host cells include a yeast cell, a plant cell, or an animal cell, such as a mammalian cell or an insect cell (e.g., a baculovirus-infectible cell such as an Sf9 or HighFive cell). Examples of suitable mammalian cells include various prostate cancer cell lines such as DU145 and TsuPr1, other transfectable or transducible prostate cancer cell lines, primary cells (PrEC), as well as a number of mammalian cells routinely used for the expression of recombinant proteins (e.g., COS, CHO, 293, 293T cells). More particularly, a polynucleotide comprising the coding sequence of 158P3D2 or a fragment, analog or homolog thereof can be used to generate 158P3D2 proteins or fragments thereof using any number of host-vector systems routinely used and widely known in the art. 
     A wide range of host-vector systems suitable for the expression of 158P3D2 proteins or fragments thereof are available, see for example, Sambrook et al., 1989, supra; Current Protocols in Molecular Biology, 1995, supra). Preferred vectors for mammalian expression include but are not limited to pcDNA 3.1 myc-His-tag (Invitrogen) and the retroviral vector pSRαtkneo (Muller et al., 1991, MCB 11:1785). Using these expression vectors, 158P3D2 can be expressed in several prostate cancer and non-prostate cell lines, including for example 293, 293T, rat-1, NIH 3T3 and TsuPr1. The host-vector systems of the invention are useful for the production of a 158P3D2 protein or fragment thereof. Such host-vector systems can be employed to study the functional properties of 158P3D2 and 158P3D2 mutations or analogs. 
     Recombinant human 158P3D2 protein or an analog or homolog or fragment thereof can be produced by mammalian cells transfected with a construct encoding a 158P3D2-related nucleotide. For example, 293T cells can be transfected with an expression plasmid encoding 158P3D2 or fragment, analog or homolog thereof, a 158P3D2-related protein is expressed in the 293T cells, and the recombinant 158P3D2 protein is isolated using standard purification methods (e.g., affinity purification using anti-158P3D2 antibodies). In another embodiment, a 158P3D2 coding sequence is subcloned into the retroviral vector pSRaMSVtkneo and used to infect various mammalian cell lines, such as NIH 3T3, TsuPr1, 293 and rat-1 in order to establish 158P3D2 expressing cell lines. Various other expression systems well known in the art can also be employed. Expression constructs encoding a leader peptide joined in frame to a 158P3D2 coding sequence can be used for the generation of a secreted form of recombinant 158P3D2 protein. 
     As discussed herein, redundancy in the genetic code permits variation in 158P3D2 gene sequences. In particular, it is known in the art that specific host species often have specific codon preferences, and thus one can adapt the disclosed sequence as preferred for a desired host. For example, preferred analog codon sequences typically have rare codons (i.e., codons having a usage frequency of less than about 20% in known sequences of the desired host) replaced with higher frequency codons. Codon preferences for a specific species are calculated, for example, by utilizing codon usage tables available on the INTERNET such as at URL dna.affrc.go.jp/˜nakamura/codon.html. 
     Additional sequence modifications are known to enhance protein expression in a cellular host. These include elimination of sequences encoding spurious polyadenylation signals, exon/intron splice site signals, transposon-like repeats, and/or other such well-characterized sequences that are deleterious to gene expression. The GC content of the sequence is adjusted to levels average for a given cellular host, as calculated by reference to known genes expressed in the host cell. Where possible, the sequence is modified to avoid predicted hairpin secondary mRNA structures. Other useful modifications include the addition of a translational initiation consensus sequence at the start of the open reading frame, as described in Kozak, Mol. Cell Biol., 9:5073-5080 (1989). Skilled artisans understand that the general rule that eukaryotic ribosomes initiate translation exclusively at the 5′ proximal AUG codon is abrogated only under rare conditions (see, e.g., Kozak PNAS 92(7): 2662-2666, (1995) and Kozak NAR 15(20): 8125-8148 (1987)). 
     III.) 158P3D2-Related Proteins 
     Another aspect of the present invention provides 158P3D2-related proteins. Specific embodiments of 158P3D2 proteins comprise a polypeptide having all or part of the amino acid sequence of human 158P3D2 as shown in  FIG. 2  or  FIG. 3 . Alternatively, embodiments of 158P3D2 proteins comprise variant, homolog or analog polypeptides that have alterations in the amino acid sequence of 158P3D2 shown in  FIG. 2  or  FIG. 3 . 
     Embodiments of a 158P3D2 polypeptide include: a 158P3D2 polypeptide having a sequence shown in  FIG. 2 , a peptide sequence of a 158P3D2 as shown in  FIG. 2  wherein T is U; at least 10 contiguous nucleotides of a polypeptide having the sequence as shown in  FIG. 2 ; or, at least 10 contiguous peptides of a polypeptide having the sequence as shown in  FIG. 2  where T is U. For example, embodiments of 158P3D2 peptides comprise, without limitation: 
     (I) a protein comprising, consisting essentially of, or consisting of an amino acid sequence as shown in  FIG. 2A-Q  or  FIG. 3A-3R ; 
     (II) a 158P3D2-related protein that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% homologous to an entire amino acid sequence shown in  FIG. 2A-Q  or  3 A-R; 
     (III) a 158P3D2-related protein that is at least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identical to an entire amino acid sequence shown in  FIG. 2A-Q  or  3 A-R; 
     (IV) a protein that comprises at least one peptide set forth in Tables VIII to XLIX, optionally with a proviso that it is not an entire protein of  FIG. 2 ; 
     (V) a protein that comprises at least one peptide set forth in Tables VIII-XXI, collectively, which peptide is also set forth in Tables XXII to XLIX, collectively, optionally with a proviso that it is not an entire protein of  FIG. 2 ; 
     (VI) a protein that comprises at least two peptides selected from the peptides set forth in Tables VIII-XLIX, optionally with a proviso that it is not an entire protein of  FIG. 2 ; 
     (VII) a protein that comprises at least two peptides selected from the peptides set forth in Tables VIII to XLIX collectively, with a proviso that the protein is not a contiguous sequence from an amino acid sequence of  FIG. 2 ; 
     (VIII) a protein that comprises at least one peptide selected from the peptides set forth in Tables VIII-XXI; and at least one peptide selected from the peptides set forth in Tables XXII to XLIX, with a proviso that the protein is not a contiguous sequence from an amino acid sequence of  FIG. 2 ; 
     (IX) a polypeptide comprising at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a protein of  FIG. 3A-3R  in any whole number increment up to 328, 236, 181, 178, 1393, 1145, 1990, 2036, 610, 1978, and 2018 respectively that includes at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value greater than 0.5 in the Hydrophilicity profile of  FIG. 5 ; 
     (X) a polypeptide comprising at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a protein of  FIG. 3A-3R  in any whole number increment up to 328, 236, 181, 178, 1393, 1145, 1990, 2036, 610, 1978, and 2018 respectively that includes at least at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value less than 0.5 in the Hydropathicity profile of  FIG. 6 ; 
     (XI) a polypeptide comprising at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a protein of  FIG. 3A-3R , in any whole number increment up to 328, 236, 181, 178, 1393, 1145, 1990, 2036, 610, 1978, and 2018 respectively that includes at least at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value greater than 0.5 in the Percent Accessible Residues profile of  FIG. 7 ; 
     (XII) a polypeptide comprising at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acids of a protein of  FIG. 3A-3R , in any whole number increment up to 328, 236, 181, 178, 1393, 1145, 1990, 2036, 610, 1978, and 2018 respectively that includes at least at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value greater than 0.5 in the Average Flexibility profile of  FIG. 8 ; 
     (XIII) a polypeptide comprising at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, amino acids of a protein of  FIG. 3A-3R  in any whole number increment up to 328, 236, 181, 178, 1393, 1145, 1990, 2036, 610, 1978, and 2018 respectively that includes at least at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 amino acid position(s) having a value greater than 0.5 in the Beta-turn profile of  FIG. 9 ; 
     (XIV) a peptide that occurs at least twice in Tables VIII-XXI and XXII to XLIX, collectively; 
     (XV) a peptide that occurs at least three times in Tables VIII-XXI and XXII to XLIX, collectively; 
     (XVI) a peptide that occurs at least four times in Tables VIII-XXI and XXII to XLIX, collectively; 
     (XVII) a peptide that occurs at least five times in Tables VIII-XXI and XXII to XLIX, collectively; 
     (XVIII) a peptide that occurs at least once in Tables VIII-XXI, and at least once in tables XXII to XLIX; 
     (XIX) a peptide that occurs at least once in Tables VIII-XXI, and at least twice in tables XXII to XLIX; 
     (XX) a peptide that occurs at least twice in Tables VIII-XXI, and at least once in tables XXII to XLIX; 
     (XXI) a peptide that occurs at least twice in Tables VIII-XXI, and at least twice in tables XXII to XLIX; 
     (XXII) a peptide which comprises one two, three, four, or five of the following characteristics, or an oligonucleotide encoding such peptide: 
     i) a region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or greater than 0.5, 0.6, 0.7, 0.8, 0.9, or having a value equal to 1.0, in the Hydrophilicity profile of  FIG. 5 ; 
     ii) a region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or less than 0.5, 0.4, 0.3, 0.2, 0.1, or having a value equal to 0.0, in the Hydropathicity profile of  FIG. 6 ; 
     iii) a region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or greater than 0.5, 0.6, 0.7, 0.8, 0.9, or having a value equal to 1.0, in the Percent Accessible Residues profile of  FIG. 7 ; 
     iv) a region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or greater than 0.5, 0.6, 0.7, 0.8, 0.9, or having a value equal to 1.0, in the Average Flexibility profile of  FIG. 8 ; or, 
     v) a region of at least 5 amino acids of a particular peptide of  FIG. 3 , in any whole number increment up to the full length of that protein in  FIG. 3 , that includes an amino acid position having a value equal to or greater than 0.5, 0.6, 0.7, 0.8, 0.9, or having a value equal to 1.0, in the Beta-turn profile of  FIG. 9 ; 
     (XXIII) a composition comprising a peptide of (I)-(XXII) or an antibody or binding region thereof together with a pharmaceutical excipient and/or in a human unit dose form. 
     (XXIV) a method of using a peptide of (I)-(XXII), or an antibody or binding region thereof or a composition of (XXIII) in a method to modulate a cell expressing 158P3D2; 
     (XXV) a method of using a peptide of (I)-(XXII) or an antibody or binding region thereof or a composition of (XXIII) in a method to diagnose, prophylax, prognose, or treat an individual who bears a cell expressing 158P3D2; 
     (XXVI) a method of using a peptide of (I)-(XXII) or an antibody or binding region thereof or a composition (XXIII) in a method to diagnose, prophylax, prognose, or treat an individual who bears a cell expressing 158P3D2, said cell from a cancer of a tissue listed in Table I; 
     (XXVII) a method of using a peptide of (I)-(XXII) or an antibody or binding region thereof or a composition of (XXIII) in a method to diagnose, prophylax, prognose, or treat a cancer; 
     (XXVIII) a method of using a peptide of (I)-(XXII) or an antibody or binding region thereof or a composition of (XXIII) in a method to diagnose, prophylax, prognose, or treat a cancer of a tissue listed in Table I; 
     (XXIX) a method of using a peptide of (I)-(XXII) or an antibody or binding region thereof or a composition and; 
     (XXIII) in a method to identify or characterize a modulator of a cell expressing 158P3D2. 
     As used herein, a range is understood to specifically disclose all whole unit positions thereof. 
     Typical embodiments of the invention disclosed herein include 158P3D2 polynucleotides that encode specific portions of 158P3D2 mRNA sequences (and those which are complementary to such sequences) such as those that encode the proteins and/or fragments thereof, for example: 
     (a) 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 180, 185, 190, 195, 200, 225, 250, 255, 260, 265, 270, 275, 280, 285, 290, 295, 300, 305, 310, 315, 320, 325, and 328 or more contiguous amino acids of 158P3D2 variant 1; the maximal lengths relevant for other variants are shown in  FIGS. 2A-2Q  and  3 A- 3 R. 
     In general, naturally occurring allelic variants of human 158P3D2 share a high degree of structural identity and homology (e.g., 90% or more homology). Typically, allelic variants of a 158P3D2 protein contain conservative amino acid substitutions within the 158P3D2 sequences described herein or contain a substitution of an amino acid from a corresponding position in a homologue of 158P3D2. One class of 158P3D2 allelic variants are proteins that share a high degree of homology with at least a small region of a particular 158P3D2 amino acid sequence, but further contain a radical departure from the sequence, such as a non-conservative substitution, truncation, insertion or frame shift. In comparisons of protein sequences, the terms, similarity, identity, and homology each have a distinct meaning as appreciated in the field of genetics. Moreover, orthology and paralogy can be important concepts describing the relationship of members of a given protein family in one organism to the members of the same family in other organisms. 
     Amino acid abbreviations are provided in Table II. Conservative amino acid substitutions can frequently be made in a protein without altering either the conformation or the function of the protein. Proteins of the invention can comprise 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 conservative substitutions. Such changes include substituting any of isoleucine (I), valine (V), and leucine (L) for any other of these hydrophobic amino acids; aspartic acid (D) for glutamic acid (E) and vice versa; glutamine (Q) for asparagine (N) and vice versa; and serine (S) for threonine (T) and vice versa. Other substitutions can also be considered conservative, depending on the environment of the particular amino acid and its role in the three-dimensional structure of the protein. For example, glycine (G) and alanine (A) can frequently be interchangeable, as can alanine (A) and valine (V). Methionine (M), which is relatively hydrophobic, can frequently be interchanged with leucine and isoleucine, and sometimes with valine. Lysine (K) and arginine (R) are frequently interchangeable in locations in which the significant feature of the amino acid residue is its charge and the differing pK&#39;s of these two amino acid residues are not significant. Still other changes can be considered “conservative” in particular environments (see, e.g. Table III herein; pages 13-15 “Biochemistry” 2nd ED. Lubert Stryer ed (Stanford University); Henikoff et al., PNAS 1992 Vol 89 10915-10919; Lei et al., J Biol Chem 1995 May 19; 270(20):11882-6). 
     Embodiments of the invention disclosed herein include a wide variety of art-accepted variants or analogs of 158P3D2 proteins such as polypeptides having amino acid insertions, deletions and substitutions. 158P3D2 variants can be made using methods known in the art such as site-directed mutagenesis, alanine scanning, and PCR mutagenesis. Site-directed mutagenesis (Carter et al., Nucl. Acids Res., 13:4331 (1986); Zoller et al., Nucl. Acids Res., 10:6487 (1987)), cassette mutagenesis (Wells et al., Gene, 34:315 (1985)), restriction selection mutagenesis (Wells et al., Philos. Trans. R. Soc. London SerA, 317:415 (1986)) or other known techniques can be performed on the cloned DNA to produce the 158P3D2 variant DNA. 
     Scanning amino acid analysis can also be employed to identify one or more amino acids along a contiguous sequence that is involved in a specific biological activity such as a protein-protein interaction. Among the preferred scanning amino acids are relatively small, neutral amino acids. Such amino acids include alanine, glycine, serine, and cysteine. Alanine is typically a preferred scanning amino acid among this group because it eliminates the side-chain beyond the beta-carbon and is less likely to alter the main-chain conformation of the variant. Alanine is also typically preferred because it is the most common amino acid. Further, it is frequently found in both buried and exposed positions (Creighton, The Proteins, (W.H. Freeman &amp; Co., N.Y.); Chothia, J. Mol. Biol., 150:1 (1976)). If alanine substitution does not yield adequate amounts of variant, an isosteric amino acid can be used. 
     As defined herein, 158P3D2 variants, analogs or homologs, have the distinguishing attribute of having at least one epitope that is “cross reactive” with a 158P3D2 protein having an amino acid sequence of  FIG. 3 . As used in this sentence, “cross reactive” means that an antibody or T cell that specifically binds to a 158P3D2 variant also specifically binds to a 158P3D2 protein having an amino acid sequence set forth in  FIG. 3 . A polypeptide ceases to be a variant of a protein shown in  FIG. 3 , when it no longer contains any epitope capable of being recognized by an antibody or T cell that specifically binds to the starting 158P3D2 protein. Those skilled in the art understand that antibodies that recognize proteins bind to epitopes of varying size, and a grouping of the order of about four or five amino acids, contiguous or not, is regarded as a typical number of amino acids in a minimal epitope. See, e.g., Nair et al., J. Immunol. 2000 165(12): 6949-6955; Hebbes et al., Mol Immunol (1989) 26(9):865-73; Schwartz et al., J Immunol (1985) 135(4):2598-608. 
     Other classes of 158P3D2-related protein variants share 70%, 75%, 80%, 85% or 90% or more similarity with an amino acid sequence of  FIG. 3 , or a fragment thereof. Another specific class of 158P3D2 protein variants or analogs comprises one or more of the 158P3D2 biological motifs described herein or presently known in the art. Thus, encompassed by the present invention are analogs of 158P3D2 fragments (nucleic or amino acid) that have altered functional (e.g. immunogenic) properties relative to the starting fragment. It is to be appreciated that motifs now or which become part of the art are to be applied to the nucleic or amino acid sequences of  FIG. 2  or  FIG. 3 . 
     As discussed herein, embodiments of the claimed invention include polypeptides containing less than the full amino acid sequence of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 . For example, representative embodiments of the invention comprise peptides/proteins having any 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more contiguous amino acids of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 . 
     Moreover, representative embodiments of the invention disclosed herein include polypeptides consisting of about amino acid 1 to about amino acid 10 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 10 to about amino acid 20 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 20 to about amino acid 30 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 30 to about amino acid 40 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 40 to about amino acid 50 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 50 to about amino acid 60 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 60 to about amino acid 70 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 70 to about amino acid 80 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 80 to about amino acid 90 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , polypeptides consisting of about amino acid 90 to about amino acid 100 of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3 , etc. throughout the entirety of a 158P3D2 amino acid sequence. Moreover, polypeptides consisting of about amino acid 1 (or 20 or 30 or 40 etc.) to about amino acid 20, (or 130, or 140 or 150 etc.) of a 158P3D2 protein shown in  FIG. 2  or  FIG. 3  are embodiments of the invention. It is to be appreciated that the starting and stopping positions in this paragraph refer to the specified position as well as that position plus or minus 5 residues. 
     158P3D2-related proteins are generated using standard peptide synthesis technology or using chemical cleavage methods well known in the art. Alternatively, recombinant methods can be used to generate nucleic acid molecules that encode a 158P3D2-related protein. In one embodiment, nucleic acid molecules provide a means to generate defined fragments of a 158P3D2 protein (or variants, homologs or analogs thereof). 
     III.A.) Motif-Bearing Protein Embodiments 
     Additional illustrative embodiments of the invention disclosed herein include 158P3D2 polypeptides comprising the amino acid residues of one or more of the biological motifs contained within a 158P3D2 polypeptide sequence set forth in  FIG. 2  or  FIG. 3 . Various motifs are known in the art, and a protein can be evaluated for the presence of such motifs by a number of publicly available Internet sites (see, e.g., URL addresses: pfam.wustl.edu/; searchlauncher.bcm.tmc.edu/seq-search/struc-predict.html; psort.ims.u-tokyo.ac.jp/; cbs.dtu.dk/; ebi.ac.uk/interpro/scan.html; expasy.ch/tools/scnpsit1.html; Epimatrix™ and Epimer™, Brown University, brown.edu/Research/TB-HIV_Lab/epimatrix/epimatrix.html; and BIMAS, bimas.dcrt.nih.gov/.). 
     Motif bearing subsequences of all 158P3D2 variant proteins are set forth and identified in Tables VIII-XXI and XXII-XLIX. 
     Table V sets forth several frequently occurring motifs based on pfam searches (see URL address pfam.wustl.edu/). The columns of Table V list (1) motif name abbreviation, (2) percent identity found amongst the different member of the motif family, (3) motif name or description and (4) most common function; location information is included if the motif is relevant for location. 
     Polypeptides comprising one or more of the 158P3D2 motifs discussed above are useful in elucidating the specific characteristics of a malignant phenotype in view of the observation that the 158P3D2 motifs discussed above are associated with growth dysregulation and because 158P3D2 is overexpressed in certain cancers (See, e.g., Table I). Casein kinase II, cAMP and camp-dependent protein kinase, and Protein Kinase C, for example, are enzymes known to be associated with the development of the malignant phenotype (see e.g. Chen et al., Lab Invest., 78(2): 165-174 (1998); Gaiddon et al., Endocrinology 136(10): 4331-4338 (1995); Hall et al., Nucleic Acids Research 24(6): 1119-1126 (1996); Peterziel et al., Oncogene 18(46): 6322-6329 (1999) and O&#39;Brian, Oncol. Rep. 5(2): 305-309 (1998)). Moreover, both glycosylation and myristoylation are protein modifications also associated with cancer and cancer progression (see e.g. Dennis et al., Biochem. Biophys. Acta 1473(1):21-34 (1999); Raju et al., Exp. Cell Res. 235(1): 145-154 (1997)). Amidation is another protein modification also associated with cancer and cancer progression (see e.g. Treston et al., J. Natl. Cancer Inst. Monogr. (13): 169-175 (1992)). 
     In another embodiment, proteins of the invention comprise one or more of the immunoreactive epitopes identified in accordance with art-accepted methods, such as the peptides set forth in Tables VIII-XXI and XXII-XLIX. CTL epitopes can be determined using specific algorithms to identify peptides within a 158P3D2 protein that are capable of optimally binding to specified HLA alleles (e.g., Table IV; Epimatrix™ and Epimer™, Brown University, URL brown.edu/Research/TB-HIV_Lab/epimatrix/epimatrix.html; and BIMAS, URL bimas.dcrt.nih.gov/.) Moreover, processes for identifying peptides that have sufficient binding affinity for HLA molecules and which are correlated with being immunogenic epitopes, are well known in the art, and are carried out without undue experimentation. In addition, processes for identifying peptides that are immunogenic epitopes, are well known in the art, and are carried out without undue experimentation either in vitro or in vivo. 
     Also known in the art are principles for creating analogs of such epitopes in order to modulate immunogenicity. For example, one begins with an epitope that bears a CTL or HTL motif (see, e.g., the HLA Class I and HLA Class II motifs/supermotifs of Table IV). The epitope is analoged by substituting out an amino acid at one of the specified positions, and replacing it with another amino acid specified for that position. For example, on the basis of residues defined in Table IV, one can substitute out a deleterious residue in favor of any other residue, such as a preferred residue; substitute a less-preferred residue with a preferred residue; or substitute an originally-occurring preferred residue with another preferred residue. Substitutions can occur at primary anchor positions or at other positions in a peptide; see, e.g., Table IV. 
     A variety of references reflect the art regarding the identification and generation of epitopes in a protein of interest as well as analogs thereof. See, for example, WO 97/33602 to Chesnut et al.; Sette, Immunogenetics 1999 50(3-4): 201-212; Sette et al., J. Immunol. 2001 166(2): 1389-1397; Sidney et al., Hum. Immunol. 1997 58(1): 12-20; Kondo et al., Immunogenetics 1997 45(4): 249-258; Sidney et al., J. Immunol. 1996 157(8): 3480-90; and Falk et al., Nature 351: 290-6 (1991); Hunt et al., Science 255:1261-3 (1992); Parker et al., J. Immunol. 149:3580-7 (1992); Parker et al., J. Immunol. 152:163-75 (1994)); Kast et al., 1994 152(8): 3904-12; Borras-Cuesta et al., Hum. Immunol. 2000 61(3): 266-278; Alexander et al., J. Immunol. 2000 164(3); 164(3): 1625-1633; Alexander et al., PMID: 7895164, UI: 95202582; O&#39;Sullivan et al., J. Immunol. 1991 147(8): 2663-2669; Alexander et al., Immunity 1994 1(9): 751-761 and Alexander et al., Immunol. Res. 1998 18(2): 79-92. 
     Related embodiments of the invention include polypeptides comprising combinations of the different motifs set forth in Table VI, and/or, one or more of the predicted CTL epitopes of Tables VIII-XXI and XXII-XLIX, and/or, one or more of the predicted HTL epitopes of Tables XLVI-XLIX, and/or, one or more of the T cell binding motifs known in the art. Preferred embodiments contain no insertions, deletions or substitutions either within the motifs or within the intervening sequences of the polypeptides. In addition, embodiments which include a number of either N-terminal and/or C-terminal amino acid residues on either side of these motifs may be desirable (to, for example, include a greater portion of the polypeptide architecture in which the motif is located). Typically, the number of N-terminal and/or C-terminal amino acid residues on either side of a motif is between about 1 to about 100 amino acid residues, preferably 5 to about 50 amino acid residues. 
     158P3D2-related proteins are embodied in many forms, preferably in isolated form. A purified 158P3D2 protein molecule will be substantially free of other proteins or molecules that impair the binding of 158P3D2 to antibody, T cell or other ligand. The nature and degree of isolation and purification will depend on the intended use. Embodiments of a 158P3D2-related proteins include purified 158P3D2-related proteins and functional, soluble 158P3D2-related proteins. In one embodiment, a functional, soluble 158P3D2 protein or fragment thereof retains the ability to be bound by antibody, T cell or other ligand. 
     The invention also provides 158P3D2 proteins comprising biologically active fragments of a 158P3D2 amino acid sequence shown in  FIG. 2  or  FIG. 3 . Such proteins exhibit properties of the starting 158P3D2 protein, such as the ability to elicit the generation of antibodies that specifically bind an epitope associated with the starting 158P3D2 protein; to be bound by such antibodies; to elicit the activation of HTL or CTL; and/or, to be recognized by HTL or CTL that also specifically bind to the starting protein. 
     158P3D2-related polypeptides that contain particularly interesting structures can be predicted and/or identified using various analytical techniques well known in the art, including, for example, the methods of Chou-Fasman, Garnier-Robson, Kyte-Doolittle, Eisenberg, Karplus-Schultz or Jameson-Wolf analysis, or based on immunogenicity. Fragments that contain such structures are particularly useful in generating subunit-specific anti-158P3D2 antibodies or T cells or in identifying cellular factors that bind to 158P3D2. For example, hydrophilicity profiles can be generated, and immunogenic peptide fragments identified, using the method of Hopp, T. P. and Woods, K. R., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:3824-3828. Hydropathicity profiles can be generated, and immunogenic peptide fragments identified, using the method of Kyte, J. and Doolittle, R. F., 1982, J. Mol. Biol. 157:105-132. Percent (%) Accessible Residues profiles can be generated, and immunogenic peptide fragments identified, using the method of Janin J., 1979, Nature 277:491-492. Average Flexibility profiles can be generated, and immunogenic peptide fragments identified, using the method of Bhaskaran R., Ponnuswamy P. K., 1988, Int. J. Pept. Protein Res. 32:242-255. Beta-turn profiles can be generated, and immunogenic peptide fragments identified, using the method of Deleage, G., Roux B., 1987, Protein Engineering 1:289-294. 
     CTL epitopes can be determined using specific algorithms to identify peptides within a 158P3D2 protein that are capable of optimally binding to specified HLA alleles (e.g., by using the SYFPEITHI site at World Wide Web URL syfpeithi.bmi-heidelberg.com/; the listings in Table IV(A)-(E); Epimatrix™ and Epimer™, Brown University, URL (brown.edu/Research/TB-HIV_Lab/epimatrix/epimatrix.html); and BIMAS, URL bimas.dcrt.nih.gov/). Illustrating this, peptide epitopes from 158P3D2 that are presented in the context of human MHC Class I molecules, e.g., HLA-A1, A2, A3, All, A24, B7 and B35 were predicted (see, e.g., Tables VIII-XXI, XXII-XLIX). Specifically, the complete amino acid sequence of the 158P3D2 protein and relevant portions of other variants, i.e., for HLA Class I predictions 9 flanking residues on either side of a point mutation or exon juction, and for HLA Class II predictions 14 flanking residues on either side of a point mutation or exon junction corresponding to that variant, were entered into the HLA Peptide Motif Search algorithm found in the Bioinformatics and Molecular Analysis Section (BIMAS) web site listed above; in addition to the site SYFPEITHI, at URL syfpeithi.bmi-heidelberg.com/. 
     The HLA peptide motif search algorithm was developed by Dr. Ken Parker based on binding of specific peptide sequences in the groove of HLA Class I molecules, in particular HLA-A2 (see, e.g., Falk et al., Nature 351: 290-6 (1991); Hunt et al., Science 255:1261-3 (1992); Parker et al., J. Immunol. 149:3580-7 (1992); Parker et al., J. Immunol. 152:163-75 (1994)). This algorithm allows location and ranking of 8-mer, 9-mer, and 10-mer peptides from a complete protein sequence for predicted binding to HLA-A2 as well as numerous other HLA Class I molecules. Many HLA class I binding peptides are 8-, 9-, 10 or 11-mers. For example, for Class I HLA-A2, the epitopes preferably contain a leucine (L) or methionine (M) at position 2 and a valine (V) or leucine (L) at the C-terminus (see, e.g., Parker et al., J. Immunol. 149:3580-7 (1992)). Selected results of 158P3D2 predicted binding peptides are shown in Tables VIII-XXI and XXII-XLIX herein. In Tables VIII-XXI and XXII-XLVII, selected candidates, 9-mers and 10-mers, for each family member are shown along with their location, the amino acid sequence of each specific peptide, and an estimated binding score. In Tables XLVI-XLIX, selected candidates, 15-mers, for each family member are shown along with their location, the amino acid sequence of each specific peptide, and an estimated binding score. The binding score corresponds to the estimated half time of dissociation of complexes containing the peptide at 37° C. at pH 6.5. Peptides with the highest binding score are predicted to be the most tightly bound to HLA Class I on the cell surface for the greatest period of time and thus represent the best immunogenic targets for T-cell recognition. 
     Actual binding of peptides to an HLA allele can be evaluated by stabilization of HLA expression on the antigen-processing defective cell line T2 (see, e.g., Xue et al., Prostate 30:73-8 (1997) and Peshwa et al., Prostate 36:129-38 (1998)) Immunogenicity of specific peptides can be evaluated in vitro by stimulation of CD8+ cytotoxic T lymphocytes (CTL) in the presence of antigen presenting cells such as dendritic cells. 
     It is to be appreciated that every epitope predicted by the BIMAS site, Epimer™ and Epimatrix™ sites, or specified by the HLA class I or class II motifs available in the art or which become part of the art such as set forth in Table IV (or determined using World Wide Web site URL syfpeithi.bmi-heidelberg.com/, or BIMAS, bimas.dcrt.nih.gov/) are to be “applied” to a 158P3D2 protein in accordance with the invention. As used in this context “applied” means that a 158P3D2 protein is evaluated, e.g., visually or by computer-based patterns finding methods, as appreciated by those of skill in the relevant art. Every subsequence of a 158P3D2 protein of 8, 9, 10, or 11 amino acid residues that bears an HLA Class I motif, or a subsequence of 9 or more amino acid residues that bear an HLA Class II motif are within the scope of the invention. 
     III.B.) Expression of 158P3D2-Related Proteins 
     In an embodiment described in the examples that follow, 158P3D2 can be conveniently expressed in cells (such as 293T cells) transfected with a commercially available expression vector such as a CMV-driven expression vector encoding 158P3D2 with a C-terminal 6×His and MYC tag (pcDNA3.1/mycHIS, Invitrogen or TagS, GenHunter Corporation, Nashville Tenn.). The TagS vector provides an IgGK secretion signal that can be used to facilitate the production of a secreted 158P3D2 protein in transfected cells. The secreted HIS-tagged 158P3D2 in the culture media can be purified, e.g., using a nickel column using standard techniques. 
     III.C.) Modifications of 158P3D2-Related Proteins 
     Modifications of 158P3D2-related proteins such as covalent modifications are included within the scope of this invention. One type of covalent modification includes reacting targeted amino acid residues of a 158P3D2 polypeptide with an organic derivatizing agent that is capable of reacting with selected side chains or the N- or C-terminal residues of a 158P3D2 protein. Another type of covalent modification of a 158P3D2 polypeptide included within the scope of this invention comprises altering the native glycosylation pattern of a protein of the invention. Another type of covalent modification of 158P3D2 comprises linking a 158P3D2 polypeptide to one of a variety of nonproteinaceous polymers, e.g., polyethylene glycol (PEG), polypropylene glycol, or polyoxyalkylenes, in the manner set forth in U.S. Pat. No. 4,640,835; 4,496,689; 4,301,144; 4,670,417; 4,791,192 or 4,179,337. 
     The 158P3D2-related proteins of the present invention can also be modified to form a chimeric molecule comprising 158P3D2 fused to another, heterologous polypeptide or amino acid sequence. Such a chimeric molecule can be synthesized chemically or recombinantly. A chimeric molecule can have a protein of the invention fused to another tumor-associated antigen or fragment thereof. Alternatively, a protein in accordance with the invention can comprise a fusion of fragments of a 158P3D2 sequence (amino or nucleic acid) such that a molecule is created that is not, through its length, directly homologous to the amino or nucleic acid sequences shown in  FIG. 2  or  FIG. 3 . Such a chimeric molecule can comprise multiples of the same subsequence of 158P3D2. A chimeric molecule can comprise a fusion of a 158P3D2-related protein with a polyhistidine epitope tag, which provides an epitope to which immobilized nickel can selectively bind, with cytokines or with growth factors. The epitope tag is generally placed at the amino- or carboxyl-terminus of a 158P3D2 protein. In an alternative embodiment, the chimeric molecule can comprise a fusion of a 158P3D2-related protein with an immunoglobulin or a particular region of an immunoglobulin. For a bivalent form of the chimeric molecule (also referred to as an “immunoadhesin”), such a fusion could be to the Fc region of an IgG molecule. The Ig fusions preferably include the substitution of a soluble (transmembrane domain deleted or inactivated) form of a 158P3D2 polypeptide in place of at least one variable region within an Ig molecule. In a preferred embodiment, the immunoglobulin fusion includes the hinge, CH2 and CH3, or the hinge, CH1, CH2 and CH3 regions of an IgGI molecule. For the production of immunoglobulin fusions see, e.g., U.S. Pat. No. 5,428,130 issued Jun. 27, 1995. 
     III.D.) Uses of 158P3D2-Related Proteins 
     The proteins of the invention have a number of different specific uses. As 158P3D2 is highly expressed in prostate and other cancers, 158P3D2-related proteins are used in methods that assess the status of 158P3D2 gene products in normal versus cancerous tissues, thereby elucidating the malignant phenotype. Typically, polypeptides from specific regions of a 158P3D2 protein are used to assess the presence of perturbations (such as deletions, insertions, point mutations etc.) in those regions (such as regions containing one or more motifs). Exemplary assays utilize antibodies or T cells targeting 158P3D2-related proteins comprising the amino acid residues of one or more of the biological motifs contained within a 158P3D2 polypeptide sequence in order to evaluate the characteristics of this region in normal versus cancerous tissues or to elicit an immune response to the epitope. Alternatively, 158P3D2-related proteins that contain the amino acid residues of one or more of the biological motifs in a 158P3D2 protein are used to screen for factors that interact with that region of 158P3D2. 
     158P3D2 protein fragments/subsequences are particularly useful in generating and characterizing domain-specific antibodies (e.g., antibodies recognizing an extracellular or intracellular epitope of a 158P3D2 protein), for identifying agents or cellular factors that bind to 158P3D2 or a particular structural domain thereof, and in various therapeutic and diagnostic contexts, including but not limited to diagnostic assays, cancer vaccines and methods of preparing such vaccines. 
     Proteins encoded by the 158P3D2 genes, or by analogs, homologs or fragments thereof, have a variety of uses, including but not limited to generating antibodies and in methods for identifying ligands and other agents and cellular constituents that bind to a 158P3D2 gene product. Antibodies raised against a 158P3D2 protein or fragment thereof are useful in diagnostic and prognostic assays, and imaging methodologies in the management of human cancers characterized by expression of 158P3D2 protein, such as those listed in Table I. Such antibodies can be expressed intracellularly and used in methods of treating patients with such cancers. 158P3D2-related nucleic acids or proteins are also used in generating HTL or CTL responses. 
     Various immunological assays useful for the detection of 158P3D2 proteins are used, including but not limited to various types of radioimmunoassays, enzyme-linked immunosorbent assays (ELISA), enzyme-linked immunofluorescent assays (ELIFA), immunocytochemical methods, and the like. Antibodies can be labeled and used as immunological imaging reagents capable of detecting 158P3D2-expressing cells (e.g., in radioscintigraphic imaging methods). 158P3D2 proteins are also particularly useful in generating cancer vaccines, as further described herein. 
     IV.) 158P3D2 Antibodies 
     Another aspect of the invention provides antibodies that bind to 158P3D2-related proteins. Preferred antibodies specifically bind to a 158P3D2-related protein and do not bind (or bind weakly) to peptides or proteins that are not 158P3D2-related proteins under physiological conditions. In this context, examples of physiological conditions include: 1) phosphate buffered saline; 2) Tris-buffered saline containing 25 mM Tris and 150 mM NaCl; or normal saline (0.9% NaCl); 4) animal serum such as human serum; or, 5) a combination of any of 1) through 4); these reactions preferably taking place at pH 7.5, alternatively in a range of pH 7.0 to 8.0, or alternatively in a range of pH 6.5 to 8.5; also, these reactions taking place at a temperature between 4° C. to 37° C. For example, antibodies that bind 158P3D2 can bind 158P3D2-related proteins such as the homologs or analogs thereof. 
     158P3D2 antibodies of the invention are particularly useful in cancer (see, e.g., Table I) diagnostic and prognostic assays, and imaging methodologies. Similarly, such antibodies are useful in the treatment, diagnosis, and/or prognosis of other cancers, to the extent 158P3D2 is also expressed or overexpressed in these other cancers. Moreover, intracellularly expressed antibodies (e.g., single chain antibodies) are therapeutically useful in treating cancers in which the expression of 158P3D2 is involved, such as advanced or metastatic prostate cancers. 
     The invention also provides various immunological assays useful for the detection and quantification of 158P3D2 and mutant 158P3D2-related proteins. Such assays can comprise one or more 158P3D2 antibodies capable of recognizing and binding a 158P3D2-related protein, as appropriate. These assays are performed within various immunological assay formats well known in the art, including but not limited to various types of radioimmunoassays, enzyme-linked immunosorbent assays (ELISA), enzyme-linked immunofluorescent assays (ELIFA), and the like. 
     Immunological non-antibody assays of the invention also comprise T cell immunogenicity assays (inhibitory or stimulatory) as well as major histocompatibility complex (MHC) binding assays. 
     In addition, immunological imaging methods capable of detecting prostate cancer and other cancers expressing 158P3D2 are also provided by the invention, including but not limited to radioscintigraphic imaging methods using labeled 158P3D2 antibodies. Such assays are clinically useful in the detection, monitoring, and prognosis of 158P3D2 expressing cancers such as prostate cancer. 
     158P3D2 antibodies are also used in methods for purifying a 158P3D2-related protein and for isolating 158P3D2 homologues and related molecules. For example, a method of purifying a 158P3D2-related protein comprises incubating a 158P3D2 antibody, which has been coupled to a solid matrix, with a lysate or other solution containing a 158P3D2-related protein under conditions that permit the 158P3D2 antibody to bind to the 158P3D2-related protein; washing the solid matrix to eliminate impurities; and eluting the 158P3D2-related protein from the coupled antibody. Other uses of 158P3D2 antibodies in accordance with the invention include generating anti-idiotypic antibodies that mimic a 158P3D2 protein. 
     Various methods for the preparation of antibodies are well known in the art. For example, antibodies can be prepared by immunizing a suitable mammalian host using a 158P3D2-related protein, peptide, or fragment, in isolated or immunoconjugated form (Antibodies: A Laboratory Manual, CSH Press, Eds., Harlow, and Lane (1988); Harlow, Antibodies, Cold Spring Harbor Press, NY (1989)). In addition, fusion proteins of 158P3D2 can also be used, such as a 158P3D2 GST-fusion protein. In a particular embodiment, a GST fusion protein comprising all or most of the amino acid sequence of  FIG. 2  or  FIG. 3  is produced, then used as an immunogen to generate appropriate antibodies. In another embodiment, a 158P3D2-related protein is synthesized and used as an immunogen. 
     In addition, naked DNA immunization techniques known in the art are used (with or without purified 158P3D2-related protein or 158P3D2 expressing cells) to generate an immune response to the encoded immunogen (for review, see Donnelly et al., 1997, Ann. Rev. Immunol. 15: 617-648). 
     The amino acid sequence of a 158P3D2 protein as shown in  FIG. 2  or  FIG. 3  can be analyzed to select specific regions of the 158P3D2 protein for generating antibodies. For example, hydrophobicity and hydrophilicity analyses of a 158P3D2 amino acid sequence are used to identify hydrophilic regions in the 158P3D2 structure. Regions of a 158P3D2 protein that show immunogenic structure, as well as other regions and domains, can readily be identified using various other methods known in the art, such as Chou-Fasman, Garnier-Robson, Kyte-Doolittle, Eisenberg, Karplus-Schultz or Jameson-Wolf analysis. Hydrophilicity profiles can be generated using the method of Hopp, T. P. and Woods, K. R., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:3824-3828. Hydropathicity profiles can be generated using the method of Kyte, J. and Doolittle, R. F., 1982, J. Mol. Biol. 157:105-132. Percent (%) Accessible Residues profiles can be generated using the method of Janin J., 1979, Nature 277:491-492. Average Flexibility profiles can be generated using the method of Bhaskaran R., Ponnuswamy P. K., 1988, Int. J. Pept. Protein Res. 32:242-255. Beta-turn profiles can be generated using the method of Deleage, G., Roux B., 1987, Protein Engineering 1:289-294. Thus, each region identified by any of these programs or methods is within the scope of the present invention. Methods for the generation of 158P3D2 antibodies are further illustrated by way of the examples provided herein. Methods for preparing a protein or polypeptide for use as an immunogen are well known in the art. Also well known in the art are methods for preparing immunogenic conjugates of a protein with a carrier, such as BSA, KLH or other carrier protein. In some circumstances, direct conjugation using, for example, carbodiimide reagents are used; in other instances linking reagents such as those supplied by Pierce Chemical Co., Rockford, Ill., are effective. Administration of a 158P3D2 immunogen is often conducted by injection over a suitable time period and with use of a suitable adjuvant, as is understood in the art. During the immunization schedule, titers of antibodies can be taken to determine adequacy of antibody formation. 
     158P3D2 monoclonal antibodies can be produced by various means well known in the art. For example, immortalized cell lines that secrete a desired monoclonal antibody are prepared using the standard hybridoma technology of Kohler and Milstein or modifications that immortalize antibody-producing B cells, as is generally known Immortalized cell lines that secrete the desired antibodies are screened by immunoassay in which the antigen is a 158P3D2-related protein. When the appropriate immortalized cell culture is identified, the cells can be expanded and antibodies produced either from in vitro cultures or from ascites fluid. 
     The antibodies or fragments of the invention can also be produced, by recombinant means. Regions that bind specifically to the desired regions of a 158P3D2 protein can also be produced in the context of chimeric or complementarity-determining region (CDR) grafted antibodies of multiple species origin. Humanized or human 158P3D2 antibodies can also be produced, and are preferred for use in therapeutic contexts. Methods for humanizing murine and other non-human antibodies, by substituting one or more of the non-human antibody CDRs for corresponding human antibody sequences, are well known (see for example, Jones et al., 1986, Nature 321: 522-525; Riechmann et al., 1988, Nature 332: 323-327; Verhoeyen et al., 1988, Science 239: 1534-1536). See also, Carter et al., 1993, Proc. Natl. Acad. Sci. USA 89: 4285 and Sims et al., 1993, J. Immunol. 151: 2296. 
     Methods for producing fully human monoclonal antibodies include phage display and transgenic methods (for review, see Vaughan et al., 1998, Nature Biotechnology 16: 535-539). Fully human 158P3D2 monoclonal antibodies can be generated using cloning technologies employing large human Ig gene combinatorial libraries (i.e., phage display) (Griffiths and Hoogenboom, Building an in vitro immune system: human antibodies from phage display libraries. In: Protein Engineering of Antibody Molecules for Prophylactic and Therapeutic Applications in Man, Clark, M. (Ed.), Nottingham Academic, pp 45-64 (1993); Burton and Barbas, Human Antibodies from combinatorial libraries. Id., pp 65-82). Fully human 158P3D2 monoclonal antibodies can also be produced using transgenic mice engineered to contain human immunoglobulin gene loci as described in PCT Patent Application WO98/24893, Kucherlapati and Jakobovits et al., published Dec. 3, 1997 (see also, Jakobovits, 1998, Exp. Opin. Invest. Drugs 7(4): 607-614; U.S. Pat. No. 6,162,963 issued 19 Dec. 2000; U.S. Pat. No. 6,150,584 issued 12 Nov. 2000; and, U.S. Pat. No. 6,114,598 issued 5 Sep. 2000). This method avoids the in vitro manipulation required with phage display technology and efficiently produces high affinity authentic human antibodies. 
     Reactivity of 158P3D2 antibodies with a 158P3D2-related protein can be established by a number of well known means, including Western blot, immunoprecipitation, ELISA, and FACS analyses using, as appropriate, 158P3D2-related proteins, 158P3D2-expressing cells or extracts thereof. A 158P3D2 antibody or fragment thereof can be labeled with a detectable marker or conjugated to a second molecule. Suitable detectable markers include, but are not limited to, a radioisotope, a fluorescent compound, a bioluminescent compound, chemiluminescent compound, a metal chelator or an enzyme. Further, bi-specific antibodies specific for two or more 158P3D2 epitopes are generated using methods generally known in the art. Homodimeric antibodies can also be generated by cross-linking techniques known in the art (e.g., Wolff et al., Cancer Res. 53: 2560-2565). 
     V.) 158P3D2 Cellular Immune Responses 
     The mechanism by which T cells recognize antigens has been delineated. Efficacious peptide epitope vaccine compositions of the invention induce a therapeutic or prophylactic immune responses in very broad segments of the world-wide population. For an understanding of the value and efficacy of compositions of the invention that induce cellular immune responses, a brief review of immunology-related technology is provided. 
     A complex of an HLA molecule and a peptidic antigen acts as the ligand recognized by HLA-restricted T cells (Buus, S. et al., Cell 47:1071, 1986; Babbitt, B. P. et al., Nature 317:359, 1985; Townsend, A. and Bodmer, H., Annu. Rev. Immunol. 7:601, 1989; Germain, R. N., Annu. Rev. Immunol. 11:403, 1993). Through the study of single amino acid substituted antigen analogs and the sequencing of endogenously bound, naturally processed peptides, critical residues that correspond to motifs required for specific binding to HLA antigen molecules have been identified and are set forth in Table IV (see also, e.g., Southwood, et al., J. Immunol. 160:3363, 1998; Rammensee, et al., Immunogenetics 41:178, 1995; Rammensee et al., SYFPEITHI, access via World Wide Web at URL (134.2.96.221/scripts.hlaserver.dll/home.htm); Sette, A. and Sidney, J. Curr. Opin. Immunol. 10:478, 1998; Engelhard, V. H., Curr. Opin. Immunol. 6:13, 1994; Sette, A. and Grey, H. M., Curr. Opin. Immunol. 4:79, 1992; Sinigaglia, F. and Hammer, J. Curr. Biol. 6:52, 1994; Ruppert et al., Cell 74:929-937, 1993; Kondo et al., J. Immunol. 155:4307-4312, 1995; Sidney et al., J. Immunol. 157:3480-3490, 1996; Sidney et al., Human Immunol. 45:79-93, 1996; Sette, A. and Sidney, J. Immunogenetics 1999 November; 50(3-4):201-12, Review). 
     Furthermore, x-ray crystallographic analyses of HLA-peptide complexes have revealed pockets within the peptide binding cleft/groove of HLA molecules which accommodate, in an allele-specific mode, residues borne by peptide ligands; these residues in turn determine the HLA binding capacity of the peptides in which they are present. (See, e.g., Madden, D. R. Annu. Rev. Immunol. 13:587, 1995; Smith, et al., Immunity 4:203, 1996; Fremont et al., Immunity 8:305, 1998; Stern et al., Structure 2:245, 1994; Jones, E. Y. Curr. Opin. Immunol. 9:75, 1997; Brown, J. H. et al., Nature 364:33, 1993; Guo, H. C. et al., Proc. Natl. Acad. Sci. USA 90:8053, 1993; Guo, H. C. et al., Nature 360:364, 1992; Silver, M. L. et al., Nature 360:367, 1992; Matsumura, M. et al., Science 257:927, 1992; Madden et al., Cell 70:1035, 1992; Fremont, D. H. et al., Science 257:919, 1992; Saper, M. A., Bjorkman, P. J. and Wiley, D.C., J. Mol. Biol. 219:277, 1991.) 
     Accordingly, the definition of class I and class II allele-specific HLA binding motifs, or class I or class II supermotifs allows identification of regions within a protein that are correlated with binding to particular HLA antigen(s). 
     Thus, by a process of HLA motif identification, candidates for epitope-based vaccines have been identified; such candidates can be further evaluated by HLA-peptide binding assays to determine binding affinity and/or the time period of association of the epitope and its corresponding HLA molecule. Additional confirmatory work can be performed to select, amongst these vaccine candidates, epitopes with preferred characteristics in terms of population coverage, and/or immunogenicity. 
     Various strategies can be utilized to evaluate cellular immunogenicity, including: 
     1) Evaluation of primary T cell cultures from normal individuals (see, e.g., Wentworth, P. A. et al., Mol. Immunol. 32:603, 1995; Celis, E. et al., Proc. Natl. Acad. Sci. USA 91:2105, 1994; Tsai, V. et al., J. Immunol. 158:1796, 1997; Kawashima, I. et al., Human Immunol. 59:1, 1998). This procedure involves the stimulation of peripheral blood lymphocytes (PBL) from normal subjects with a test peptide in the presence of antigen presenting cells in vitro over a period of several weeks. T cells specific for the peptide become activated during this time and are detected using, e.g., a lymphokine- or 51Cr-release assay involving peptide sensitized target cells. 
     2) Immunization of HLA transgenic mice (see, e.g., Wentworth, P. A. et al., J. Immunol. 26:97, 1996; Wentworth, P. A. et al., Int. Immunol. 8:651, 1996; Alexander, J. et al., J. Immunol. 159:4753, 1997). For example, in such methods peptides in incomplete Freund&#39;s adjuvant are administered subcutaneously to HLA transgenic mice. Several weeks following immunization, splenocytes are removed and cultured in vitro in the presence of test peptide for approximately one week. Peptide-specific T cells are detected using, e.g., a 51Cr-release assay involving peptide sensitized target cells and target cells expressing endogenously generated antigen. 
     3) Demonstration of recall T cell responses from immune individuals who have been either effectively vaccinated and/or from chronically ill patients (see, e.g., Rehermann, B. et al., J. Exp. Med. 181:1047, 1995; Doolan, D. L. et al., Immunity 7:97, 1997; Bertoni, R. et al., J. Clin. Invest. 100:503, 1997; Threlkeld, S. C. et al., J. Immunol. 159:1648, 1997; Diepolder, H. M. et al., J. Virol. 71:6011, 1997). Accordingly, recall responses are detected by culturing PBL from subjects that have been exposed to the antigen due to disease and thus have generated an immune response “naturally”, or from patients who were vaccinated against the antigen. PBL from subjects are cultured in vitro for 1-2 weeks in the presence of test peptide plus antigen presenting cells (APC) to allow activation of “memory” T cells, as compared to “naive” T cells. At the end of the culture period, T cell activity is detected using assays including 51Cr release involving peptide-sensitized targets, T cell proliferation, or lymphokine release. 
     VI.) 158P3D2 Transgenic Animals 
     Nucleic acids that encode a 158P3D2-related protein can also be used to generate either transgenic animals or “knock out” animals that, in turn, are useful in the development and screening of therapeutically useful reagents. In accordance with established techniques, cDNA encoding 158P3D2 can be used to clone genomic DNA that encodes 158P3D2. The cloned genomic sequences can then be used to generate transgenic animals containing cells that express DNA that encode 158P3D2. Methods for generating transgenic animals, particularly animals such as mice or rats, have become conventional in the art and are described, for example, in U.S. Pat. No. 4,736,866 issued 12 Apr. 1988, and U.S. Pat. No. 4,870,009 issued 26 Sep. 1989. Typically, particular cells would be targeted for 158P3D2 transgene incorporation with tissue-specific enhancers. 
     Transgenic animals that include a copy of a transgene encoding 158P3D2 can be used to examine the effect of increased expression of DNA that encodes 158P3D2. Such animals can be used as tester animals for reagents thought to confer protection from, for example, pathological conditions associated with its overexpression. In accordance with this aspect of the invention, an animal is treated with a reagent and a reduced incidence of a pathological condition, compared to untreated animals that bear the transgene, would indicate a potential therapeutic intervention for the pathological condition. 
     Alternatively, non-human homologues of 158P3D2 can be used to construct a 158P3D2 “knock out” animal that has a defective or altered gene encoding 158P3D2 as a result of homologous recombination between the endogenous gene encoding 158P3D2 and altered genomic DNA encoding 158P3D2 introduced into an embryonic cell of the animal. For example, cDNA that encodes 158P3D2 can be used to clone genomic DNA encoding 158P3D2 in accordance with established techniques. A portion of the genomic DNA encoding 158P3D2 can be deleted or replaced with another gene, such as a gene encoding a selectable marker that can be used to monitor integration. Typically, several kilobases of unaltered flanking DNA (both at the 5′ and 3′ ends) are included in the vector (see, e.g., Thomas and Capecchi, Cell, 51:503 (1987) for a description of homologous recombination vectors). The vector is introduced into an embryonic stem cell line (e.g., by electroporation) and cells in which the introduced DNA has homologously recombined with the endogenous DNA are selected (see, e.g., Li et al., Cell, 69:915 (1992)). The selected cells are then injected into a blastocyst of an animal (e.g., a mouse or rat) to form aggregation chimeras (see, e.g., Bradley, in Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, E. J. Robertson, ed. (IRL, Oxford, 1987), pp. 113-152). A chimeric embryo can then be implanted into a suitable pseudopregnant female foster animal, and the embryo brought to term to create a “knock out” animal. Progeny harboring the homologously recombined DNA in their germ cells can be identified by standard techniques and used to breed animals in which all cells of the animal contain the homologously recombined DNA. Knock out animals can be characterized, for example, for their ability to defend against certain pathological conditions or for their development of pathological conditions due to absence of a 158P3D2 polypeptide. 
     VII.) Methods for the Detection of 158P3D2 
     Another aspect of the present invention relates to methods for detecting 158P3D2 polynucleotides and 158P3D2-related proteins, as well as methods for identifying a cell that expresses 158P3D2. The expression profile of 158P3D2 makes it a diagnostic marker for metastasized disease. Accordingly, the status of 158P3D2 gene products provides information useful for predicting a variety of factors including susceptibility to advanced stage disease, rate of progression, and/or tumor aggressiveness. As discussed in detail herein, the status of 158P3D2 gene products in patient samples can be analyzed by a variety protocols that are well known in the art including immunohistochemical analysis, the variety of Northern blotting techniques including in situ hybridization, RT-PCR analysis (for example on laser capture micro-dissected samples), Western blot analysis and tissue array analysis. 
     More particularly, the invention provides assays for the detection of 158P3D2 polynucleotides in a biological sample, such as serum, bone, prostate, and other tissues, urine, semen, cell preparations, and the like. Detectable 158P3D2 polynucleotides include, for example, a 158P3D2 gene or fragment thereof, 158P3D2 mRNA, alternative splice variant 158P3D2 mRNAs, and recombinant DNA or RNA molecules that contain a 158P3D2 polynucleotide. A number of methods for amplifying and/or detecting the presence of 158P3D2 polynucleotides are well known in the art and can be employed in the practice of this aspect of the invention. 
     In one embodiment, a method for detecting a 158P3D2 mRNA in a biological sample comprises producing cDNA from the sample by reverse transcription using at least one primer; amplifying the cDNA so produced using a 158P3D2 polynucleotides as sense and antisense primers to amplify 158P3D2 cDNAs therein; and detecting the presence of the amplified 158P3D2 cDNA. Optionally, the sequence of the amplified 158P3D2 cDNA can be determined 
     In another embodiment, a method of detecting a 158P3D2 gene in a biological sample comprises first isolating genomic DNA from the sample; amplifying the isolated genomic DNA using 158P3D2 polynucleotides as sense and antisense primers; and detecting the presence of the amplified 158P3D2 gene. Any number of appropriate sense and antisense probe combinations can be designed from a 158P3D2 nucleotide sequence (see, e.g.,  FIG. 2 ) and used for this purpose. 
     The invention also provides assays for detecting the presence of a 158P3D2 protein in a tissue or other biological sample such as serum, semen, bone, prostate, urine, cell preparations, and the like. Methods for detecting a 158P3D2-related protein are also well known and include, for example, immunoprecipitation, immunohistochemical analysis, Western blot analysis, molecular binding assays, ELISA, ELIFA and the like. For example, a method of detecting the presence of a 158P3D2-related protein in a biological sample comprises first contacting the sample with a 158P3D2 antibody, a 158P3D2-reactive fragment thereof, or a recombinant protein containing an antigen-binding region of a 158P3D2 antibody; and then detecting the binding of 158P3D2-related protein in the sample. 
     Methods for identifying a cell that expresses 158P3D2 are also within the scope of the invention. In one embodiment, an assay for identifying a cell that expresses a 158P3D2 gene comprises detecting the presence of 158P3D2 mRNA in the cell. Methods for the detection of particular mRNAs in cells are well known and include, for example, hybridization assays using complementary DNA probes (such as in situ hybridization using labeled 158P3D2 riboprobes, Northern blot and related techniques) and various nucleic acid amplification assays (such as RT-PCR using complementary primers specific for 158P3D2, and other amplification type detection methods, such as, for example, branched DNA, SISBA, TMA and the like). Alternatively, an assay for identifying a cell that expresses a 158P3D2 gene comprises detecting the presence of 158P3D2-related protein in the cell or secreted by the cell. Various methods for the detection of proteins are well known in the art and are employed for the detection of 158P3D2-related proteins and cells that express 158P3D2-related proteins. 
     158P3D2 expression analysis is also useful as a tool for identifying and evaluating agents that modulate 158P3D2 gene expression. For example, 158P3D2 expression is significantly upregulated in prostate cancer, and is expressed in cancers of the tissues listed in Table I. Identification of a molecule or biological agent that inhibits 158P3D2 expression or over-expression in cancer cells is of therapeutic value. For example, such an agent can be identified by using a screen that quantifies 158P3D2 expression by RT-PCR, nucleic acid hybridization or antibody binding. 
     VIII.) Methods for Monitoring the Status of 158P3D2-Related Genes and their Products 
     Oncogenesis is known to be a multistep process where cellular growth becomes progressively dysregulated and cells progress from a normal physiological state to precancerous and then cancerous states (see, e.g., Alers et al., Lab Invest. 77(5): 437-438 (1997) and Isaacs et al., Cancer Surv. 23: 19-32 (1995)). In this context, examining a biological sample for evidence of dysregulated cell growth (such as aberrant 158P3D2 expression in cancers) allows for early detection of such aberrant physiology, before a pathologic state such as cancer has progressed to a stage that therapeutic options are more limited and or the prognosis is worse. In such examinations, the status of 158P3D2 in a biological sample of interest can be compared, for example, to the status of 158P3D2 in a corresponding normal sample (e.g. a sample from that individual or alternatively another individual that is not affected by a pathology). An alteration in the status of 158P3D2 in the biological sample (as compared to the normal sample) provides evidence of dysregulated cellular growth. In addition to using a biological sample that is not affected by a pathology as a normal sample, one can also use a predetermined normative value such as a predetermined normal level of mRNA expression (see, e.g., Greyer et al., J. Comp. Neurol. 1996 Dec. 9; 376(2): 306-14 and U.S. Pat. No. 5,837,501) to compare 158P3D2 status in a sample. 
     The term “status” in this context is used according to its art accepted meaning and refers to the condition or state of a gene and its products. Typically, skilled artisans use a number of parameters to evaluate the condition or state of a gene and its products. These include, but are not limited to the location of expressed gene products (including the location of 158P3D2 expressing cells) as well as the level, and biological activity of expressed gene products (such as 158P3D2 mRNA, polynucleotides and polypeptides). Typically, an alteration in the status of 158P3D2 comprises a change in the location of 158P3D2 and/or 158P3D2 expressing cells and/or an increase in 158P3D2 mRNA and/or protein expression. 
     158P3D2 status in a sample can be analyzed by a number of means well known in the art, including without limitation, immunohistochemical analysis, in situ hybridization, RT-PCR analysis on laser capture micro-dissected samples, Western blot analysis, and tissue array analysis. Typical protocols for evaluating the status of a 158P3D2 gene and gene products are found, for example in Ausubel et al. eds., 1995, Current Protocols In Molecular Biology, Units 2 (Northern Blotting), 4 (Southern Blotting), 15 (Immunoblotting) and 18 (PCR Analysis). Thus, the status of 158P3D2 in a biological sample is evaluated by various methods utilized by skilled artisans including, but not limited to genomic Southern analysis (to examine, for example perturbations in a 158P3D2 gene), Northern analysis and/or PCR analysis of 158P3D2 mRNA (to examine, for example alterations in the polynucleotide sequences or expression levels of 158P3D2 mRNAs), and, Western and/or immunohistochemical analysis (to examine, for example alterations in polypeptide sequences, alterations in polypeptide localization within a sample, alterations in expression levels of 158P3D2 proteins and/or associations of 158P3D2 proteins with polypeptide binding partners). Detectable 158P3D2 polynucleotides include, for example, a 158P3D2 gene or fragment thereof, 158P3D2 mRNA, alternative splice variants, 158P3D2 mRNAs, and recombinant DNA or RNA molecules containing a 158P3D2 polynucleotide. 
     The expression profile of 158P3D2 makes it a diagnostic marker for local and/or metastasized disease, and provides information on the growth or oncogenic potential of a biological sample. In particular, the status of 158P3D2 provides information useful for predicting susceptibility to particular disease stages, progression, and/or tumor aggressiveness. The invention provides methods and assays for determining 158P3D2 status and diagnosing cancers that express 158P3D2, such as cancers of the tissues listed in Table I. For example, because 158P3D2 mRNA is so highly expressed in prostate and other cancers relative to normal prostate tissue, assays that evaluate the levels of 158P3D2 mRNA transcripts or proteins in a biological sample can be used to diagnose a disease associated with 158P3D2 dysregulation, and can provide prognostic information useful in defining appropriate therapeutic options. 
     The expression status of 158P3D2 provides information including the presence, stage and location of dysplastic, precancerous and cancerous cells, predicting susceptibility to various stages of disease, and/or for gauging tumor aggressiveness. Moreover, the expression profile makes it useful as an imaging reagent for metastasized disease. Consequently, an aspect of the invention is directed to the various molecular prognostic and diagnostic methods for examining the status of 158P3D2 in biological samples such as those from individuals suffering from, or suspected of suffering from a pathology characterized by dysregulated cellular growth, such as cancer. 
     As described above, the status of 158P3D2 in a biological sample can be examined by a number of well-known procedures in the art. For example, the status of 158P3D2 in a biological sample taken from a specific location in the body can be examined by evaluating the sample for the presence or absence of 158P3D2 expressing cells (e.g. those that express 158P3D2 mRNAs or proteins). This examination can provide evidence of dysregulated cellular growth, for example, when 158P3D2-expressing cells are found in a biological sample that does not normally contain such cells (such as a lymph node), because such alterations in the status of 158P3D2 in a biological sample are often associated with dysregulated cellular growth. Specifically, one indicator of dysregulated cellular growth is the metastases of cancer cells from an organ of origin (such as the prostate) to a different area of the body (such as a lymph node). In this context, evidence of dysregulated cellular growth is important for example because occult lymph node metastases can be detected in a substantial proportion of patients with prostate cancer, and such metastases are associated with known predictors of disease progression (see, e.g., Murphy et al., Prostate 42(4): 315-317 (2000); Su et al., Semin Surg. Oncol. 18(1): 17-28 (2000) and Freeman et al., J Urol 1995 August 154(2 Pt 1):474-8). 
     In one aspect, the invention provides methods for monitoring 158P3D2 gene products by determining the status of 158P3D2 gene products expressed by cells from an individual suspected of having a disease associated with dysregulated cell growth (such as hyperplasia or cancer) and then comparing the status so determined to the status of 158P3D2 gene products in a corresponding normal sample. The presence of aberrant 158P3D2 gene products in the test sample relative to the normal sample provides an indication of the presence of dysregulated cell growth within the cells of the individual. 
     In another aspect, the invention provides assays useful in determining the presence of cancer in an individual, comprising detecting a significant increase in 158P3D2 mRNA or protein expression in a test cell or tissue sample relative to expression levels in the corresponding normal cell or tissue. The presence of 158P3D2 mRNA can, for example, be evaluated in tissues including but not limited to those listed in Table I. The presence of significant 158P3D2 expression in any of these tissues is useful to indicate the emergence, presence and/or severity of a cancer, since the corresponding normal tissues do not express 158P3D2 mRNA or express it at lower levels. 
     In a related embodiment, 158P3D2 status is determined at the protein level rather than at the nucleic acid level. For example, such a method comprises determining the level of 158P3D2 protein expressed by cells in a test tissue sample and comparing the level so determined to the level of 158P3D2 expressed in a corresponding normal sample. In one embodiment, the presence of 158P3D2 protein is evaluated, for example, using immunohistochemical methods. 158P3D2 antibodies or binding partners capable of detecting 158P3D2 protein expression are used in a variety of assay formats well known in the art for this purpose. 
     In a further embodiment, one can evaluate the status of 158P3D2 nucleotide and amino acid sequences in a biological sample in order to identify perturbations in the structure of these molecules. These perturbations can include insertions, deletions, substitutions and the like. Such evaluations are useful because perturbations in the nucleotide and amino acid sequences are observed in a large number of proteins associated with a growth dysregulated phenotype (see, e.g., Marrogi et al., 1999, J. Cutan. Pathol. 26(8):369-378). For example, a mutation in the sequence of 158P3D2 may be indicative of the presence or promotion of a tumor. Such assays therefore have diagnostic and predictive value where a mutation in 158P3D2 indicates a potential loss of function or increase in tumor growth. 
     A wide variety of assays for observing perturbations in nucleotide and amino acid sequences are well known in the art. For example, the size and structure of nucleic acid or amino acid sequences of 158P3D2 gene products are observed by the Northern, Southern, Western, PCR and DNA sequencing protocols discussed herein. In addition, other methods for observing perturbations in nucleotide and amino acid sequences such as single strand conformation polymorphism analysis are well known in the art (see, e.g., U.S. Pat. No. 5,382,510 issued 7 Sep. 1999, and U.S. Pat. No. 5,952,170 issued 17 Jan. 1995). 
     Additionally, one can examine the methylation status of a 158P3D2 gene in a biological sample. Aberrant demethylation and/or hypermethylation of CpG islands in gene 5′ regulatory regions frequently occurs in immortalized and transformed cells, and can result in altered expression of various genes. For example, promoter hypermethylation of the pi-class glutathione S-transferase (a protein expressed in normal prostate but not expressed in &gt;90% of prostate carcinomas) appears to permanently silence transcription of this gene and is the most frequently detected genomic alteration in prostate carcinomas (De Marzo et al., Am. J. Pathol. 155(6): 1985-1992 (1999)). In addition, this alteration is present in at least 70% of cases of high-grade prostatic intraepithelial neoplasia (PIN) (Brooks et al., Cancer Epidemiol. Biomarkers Prey., 1998, 7:531-536). In another example, expression of the LAGE-I tumor specific gene (which is not expressed in normal prostate but is expressed in 25-50% of prostate cancers) is induced by deoxy-azacytidine in lymphoblastoid cells, suggesting that tumoral expression is due to demethylation (Lethe et al., Int. J. Cancer 76(6): 903-908 (1998)). A variety of assays for examining methylation status of a gene are well known in the art. For example, one can utilize, in Southern hybridization approaches, methylation-sensitive restriction enzymes that cannot cleave sequences that contain methylated CpG sites to assess the methylation status of CpG islands. In addition, MSP (methylation specific PCR) can rapidly profile the methylation status of all the CpG sites present in a CpG island of a given gene. This procedure involves initial modification of DNA by sodium bisulfite (which will convert all unmethylated cytosines to uracil) followed by amplification using primers specific for methylated versus unmethylated DNA. Protocols involving methylation interference can also be found for example in Current Protocols In Molecular Biology, Unit 12, Frederick M. Ausubel et al. eds., 1995. 
     Gene amplification is an additional method for assessing the status of 158P3D2. Gene amplification is measured in a sample directly, for example, by conventional Southern blotting or Northern blotting to quantitate the transcription of mRNA (Thomas, 1980, Proc. Natl. Acad. Sci. USA, 77:5201-5205), dot blotting (DNA analysis), or in situ hybridization, using an appropriately labeled probe, based on the sequences provided herein. Alternatively, antibodies are employed that recognize specific duplexes, including DNA duplexes, RNA duplexes, and DNA-RNA hybrid duplexes or DNA-protein duplexes. The antibodies in turn are labeled and the assay carried out where the duplex is bound to a surface, so that upon the formation of duplex on the surface, the presence of antibody bound to the duplex can be detected. 
     Biopsied tissue or peripheral blood can be conveniently assayed for the presence of cancer cells using for example, Northern, dot blot or RT-PCR analysis to detect 158P3D2 expression. The presence of RT-PCR amplifiable 158P3D2 mRNA provides an indication of the presence of cancer. RT-PCR assays are well known in the art. RT-PCR detection assays for tumor cells in peripheral blood are currently being evaluated for use in the diagnosis and management of a number of human solid tumors. In the prostate cancer field, these include RT-PCR assays for the detection of cells expressing PSA and PSM (Verkaik et al., 1997, Urol. Res. 25:373-384; Ghossein et al., 1995, J. Clin. Oncol. 13:1195-2000; Heston et al., 1995, Clin. Chem. 41:1687-1688). 
     A further aspect of the invention is an assessment of the susceptibility that an individual has for developing cancer. In one embodiment, a method for predicting susceptibility to cancer comprises detecting 158P3D2 mRNA or 158P3D2 protein in a tissue sample, its presence indicating susceptibility to cancer, wherein the degree of 158P3D2 mRNA expression correlates to the degree of susceptibility. In a specific embodiment, the presence of 158P3D2 in prostate or other tissue is examined, with the presence of 158P3D2 in the sample providing an indication of prostate cancer susceptibility (or the emergence or existence of a prostate tumor). Similarly, one can evaluate the integrity 158P3D2 nucleotide and amino acid sequences in a biological sample, in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like. The presence of one or more perturbations in 158P3D2 gene products in the sample is an indication of cancer susceptibility (or the emergence or existence of a tumor). 
     The invention also comprises methods for gauging tumor aggressiveness. In one embodiment, a method for gauging aggressiveness of a tumor comprises determining the level of 158P3D2 mRNA or 158P3D2 protein expressed by tumor cells, comparing the level so determined to the level of 158P3D2 mRNA or 158P3D2 protein expressed in a corresponding normal tissue taken from the same individual or a normal tissue reference sample, wherein the degree of 158P3D2 mRNA or 158P3D2 protein expression in the tumor sample relative to the normal sample indicates the degree of aggressiveness. In a specific embodiment, aggressiveness of a tumor is evaluated by determining the extent to which 158P3D2 is expressed in the tumor cells, with higher expression levels indicating more aggressive tumors. Another embodiment is the evaluation of the integrity of 158P3D2 nucleotide and amino acid sequences in a biological sample, in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like. The presence of one or more perturbations indicates more aggressive tumors. 
     Another embodiment of the invention is directed to methods for observing the progression of a malignancy in an individual over time. In one embodiment, methods for observing the progression of a malignancy in an individual over time comprise determining the level of 158P3D2 mRNA or 158P3D2 protein expressed by cells in a sample of the tumor, comparing the level so determined to the level of 158P3D2 mRNA or 158P3D2 protein expressed in an equivalent tissue sample taken from the same individual at a different time, wherein the degree of 158P3D2 mRNA or 158P3D2 protein expression in the tumor sample over time provides information on the progression of the cancer. In a specific embodiment, the progression of a cancer is evaluated by determining 158P3D2 expression in the tumor cells over time, where increased expression over time indicates a progression of the cancer. Also, one can evaluate the integrity 158P3D2 nucleotide and amino acid sequences in a biological sample in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like, where the presence of one or more perturbations indicates a progression of the cancer. 
     The above diagnostic approaches can be combined with any one of a wide variety of prognostic and diagnostic protocols known in the art. For example, another embodiment of the invention is directed to methods for observing a coincidence between the expression of 158P3D2 gene and 158P3D2 gene products (or perturbations in 158P3D2 gene and 158P3D2 gene products) and a factor that is associated with malignancy, as a means for diagnosing and prognosticating the status of a tissue sample. A wide variety of factors associated with malignancy can be utilized, such as the expression of genes associated with malignancy (e.g. PSA, PSCA and PSM expression for prostate cancer etc.) as well as gross cytological observations (see, e.g., Bocking et al., 1984, Anal. Quant. Cytol. 6(2):74-88; Epstein, 1995, Hum. Pathol. 26(2):223-9; Thorson et al., 1998, Mod. Pathol. 11(6):543-51; Baisden et al., 1999, Am. J. Surg. Pathol. 23(8):918-24). Methods for observing a coincidence between the expression of 158P3D2 gene and 158P3D2 gene products (or perturbations in 158P3D2 gene and 158P3D2 gene products) and another factor that is associated with malignancy are useful, for example, because the presence of a set of specific factors that coincide with disease provides information crucial for diagnosing and prognosticating the status of a tissue sample. 
     In one embodiment, methods for observing a coincidence between the expression of 158P3D2 gene and 158P3D2 gene products (or perturbations in 158P3D2 gene and 158P3D2 gene products) and another factor associated with malignancy entails detecting the overexpression of 158P3D2 mRNA or protein in a tissue sample, detecting the overexpression of PSA mRNA or protein in a tissue sample (or PSCA or PSM expression), and observing a coincidence of 158P3D2 mRNA or protein and PSA mRNA or protein overexpression (or PSCA or PSM expression). In a specific embodiment, the expression of 158P3D2 and PSA mRNA in prostate tissue is examined, where the coincidence of 158P3D2 and PSA mRNA overexpression in the sample indicates the existence of prostate cancer, prostate cancer susceptibility or the emergence or status of a prostate tumor. 
     Methods for detecting and quantifying the expression of 158P3D2 mRNA or protein are described herein, and standard nucleic acid and protein detection and quantification technologies are well known in the art. Standard methods for the detection and quantification of 158P3D2 mRNA include in situ hybridization using labeled 158P3D2 riboprobes, Northern blot and related techniques using 158P3D2 polynucleotide probes, RT-PCR analysis using primers specific for 158P3D2, and other amplification type detection methods, such as, for example, branched DNA, SISBA, TMA and the like. In a specific embodiment, semi-quantitative RT-PCR is used to detect and quantify 158P3D2 mRNA expression. Any number of primers capable of amplifying 158P3D2 can be used for this purpose, including but not limited to the various primer sets specifically described herein. In a specific embodiment, polyclonal or monoclonal antibodies specifically reactive with the wild-type 158P3D2 protein can be used in an immunohistochemical assay of biopsied tissue. 
     IX.) Identification of Molecules that Interact with 158P3D2 
     The 158P3D2 protein and nucleic acid sequences disclosed herein allow a skilled artisan to identify proteins, small molecules and other agents that interact with 158P3D2, as well as pathways activated by 158P3D2 via any one of a variety of art accepted protocols. For example, one can utilize one of the so-called interaction trap systems (also referred to as the “two-hybrid assay”). In such systems, molecules interact and reconstitute a transcription factor which directs expression of a reporter gene, whereupon the expression of the reporter gene is assayed. Other systems identify protein-protein interactions in vivo through reconstitution of a eukaryotic transcriptional activator, see, e.g., U.S. Pat. No. 5,955,280 issued 21 Sep. 1999, U.S. Pat. No. 5,925,523 issued 20 Jul. 1999, U.S. Pat. No. 5,846,722 issued 8 Dec. 1998 and U.S. Pat. No. 6,004,746 issued 21 Dec. 1999. Algorithms are also available in the art for genome-based predictions of protein function (see, e.g., Marcotte, et al., Nature 402: 4 Nov. 1999, 83-86). 
     Alternatively one can screen peptide libraries to identify molecules that interact with 158P3D2 protein sequences. In such methods, peptides that bind to 158P3D2 are identified by screening libraries that encode a random or controlled collection of amino acids. Peptides encoded by the libraries are expressed as fusion proteins of bacteriophage coat proteins, the bacteriophage particles are then screened against the 158P3D2 protein(s). 
     Accordingly, peptides having a wide variety of uses, such as therapeutic, prognostic or diagnostic reagents, are thus identified without any prior information on the structure of the expected ligand or receptor molecule. Typical peptide libraries and screening methods that can be used to identify molecules that interact with 158P3D2 protein sequences are disclosed for example in U.S. Pat. No. 5,723,286 issued 3 Mar. 1998 and U.S. Pat. No. 5,733,731 issued 31 Mar. 1998. 
     Alternatively, cell lines that express 158P3D2 are used to identify protein-protein interactions mediated by 158P3D2. Such interactions can be examined using immunoprecipitation techniques (see, e.g., Hamilton B. J., et al. Biochem. Biophys. Res. Commun 1999, 261:646-51). 158P3D2 protein can be immunoprecipitated from 158P3D2-expressing cell lines using anti-158P3D2 antibodies. Alternatively, antibodies against His-tag can be used in a cell line engineered to express fusions of 158P3D2 and a His-tag (vectors mentioned above). The immunoprecipitated complex can be examined for protein association by procedures such as Western blotting, 35S-methionine labeling of proteins, protein microsequencing, silver staining and two-dimensional gel electrophoresis. 
     Small molecules and ligands that interact with 158P3D2 can be identified through related embodiments of such screening assays. For example, small molecules can be identified that interfere with protein function, including molecules that interfere with 158P3D2&#39;s ability to mediate phosphorylation and de-phosphorylation, interaction with DNA or RNA molecules as an indication of regulation of cell cycles, second messenger signaling or tumorigenesis. Similarly, small molecules that modulate 158P3D2-related ion channel, protein pump, or cell communication functions are identified and used to treat patients that have a cancer that expresses 158P3D2 (see, e.g., Hille, B., Ionic Channels of Excitable Membranes 2nd Ed., Sinauer Assoc., Sunderland, Mass., 1992). Moreover, ligands that regulate 158P3D2 function can be identified based on their ability to bind 158P3D2 and activate a reporter construct. Typical methods are discussed for example in U.S. Pat. No. 5,928,868 issued 27 Jul. 1999, and include methods for forming hybrid ligands in which at least one ligand is a small molecule. In an illustrative embodiment, cells engineered to express a fusion protein of 158P3D2 and a DNA-binding protein are used to co-express a fusion protein of a hybrid ligand/small molecule and a cDNA library transcriptional activator protein. The cells further contain a reporter gene, the expression of which is conditioned on the proximity of the first and second fusion proteins to each other, an event that occurs only if the hybrid ligand binds to target sites on both hybrid proteins. Those cells that express the reporter gene are selected and the unknown small molecule or the unknown ligand is identified. This method provides a means of identifying modulators, which activate or inhibit 158P3D2. 
     An embodiment of this invention comprises a method of screening for a molecule that interacts with a 158P3D2 amino acid sequence shown in  FIG. 2  or  FIG. 3 , comprising the steps of contacting a population of molecules with a 158P3D2 amino acid sequence, allowing the population of molecules and the 158P3D2 amino acid sequence to interact under conditions that facilitate an interaction, determining the presence of a molecule that interacts with the 158P3D2 amino acid sequence, and then separating molecules that do not interact with the 158P3D2 amino acid sequence from molecules that do. In a specific embodiment, the method further comprises purifying, characterizing and identifying a molecule that interacts with the 158P3D2 amino acid sequence. The identified molecule can be used to modulate a function performed by 158P3D2. In a preferred embodiment, the 158P3D2 amino acid sequence is contacted with a library of peptides. 
     X.) Therapeutic Methods and Compositions 
     The identification of 158P3D2 as a protein that is normally expressed in a restricted set of tissues, but which is also expressed in cancers such as those listed in Table I, opens a number of therapeutic approaches to the treatment of such cancers. 
     Of note, targeted antitumor therapies have been useful even when the targeted protein is expressed on normal tissues, even vital normal organ tissues. A vital organ is one that is necessary to sustain life, such as the heart or colon. A non-vital organ is one that can be removed whereupon the individual is still able to survive. Examples of non-vital organs are ovary, breast, and prostate. 
     For example, Herceptin® is an FDA approved pharmaceutical that has as its active ingredient an antibody which is immunoreactive with the protein variously known as HER2, HER2/neu, and erb-b-2. It is marketed by Genentech and has been a commercially successful antitumor agent. Herceptin sales reached almost $400 million in 2002. Herceptin is a treatment for HER2 positive metastatic breast cancer. However, the expression of HER2 is not limited to such tumors. The same protein is expressed in a number of normal tissues. In particular, it is known that HER2/neu is present in normal kidney and heart, thus these tissues are present in all human recipients of Herceptin. The presence of HER2/neu in normal kidney is also confirmed by Latif, Z., et al., B.J.U. International (2002) 89:5-9. As shown in this article (which evaluated whether renal cell carcinoma should be a preferred indication for anti-HER2 antibodies such as Herceptin) both protein and mRNA are produced in benign renal tissues. Notably, HER2/neu protein was strongly overexpressed in benign renal tissue. 
     Despite the fact that HER2/neu is expressed in such vital tissues as heart and kidney, Herceptin is a very useful, FDA approved, and commercially successful drug. The effect of Herceptin on cardiac tissue, i.e., “cardiotoxicity,” has merely been a side effect to treatment. When patients were treated with Herceptin alone, significant cardiotoxicity occurred in a very low percentage of patients. 
     Of particular note, although kidney tissue is indicated to exhibit normal expression, possibly even higher expression than cardiac tissue, kidney has no appreciable Herceptin side effect whatsoever. Moreover, of the diverse array of normal tissues in which HER2 is expressed, there is very little occurrence of any side effect. Only cardiac tissue has manifested any appreciable side effect at all. A tissue such as kidney, where HER2/neu expression is especially notable, has not been the basis for any side effect. 
     Furthermore, favorable therapeutic effects have been found for antitumor therapies that target epidermal growth factor receptor (EGFR). EGFR is also expressed in numerous normal tissues. There have been very limited side effects in normal tissues following use of anti-EGFR therapeutics. 
     Thus, expression of a target protein in normal tissue, even vital normal tissue, does not defeat the utility of a targeting agent for the protein as a therapeutic for certain tumors in which the protein is also overexpressed. 
     Accordingly, therapeutic approaches that inhibit the activity of a 158P3D2 protein are useful for patients suffering from a cancer that expresses 158P3D2. These therapeutic approaches generally fall into two classes. One class comprises various methods for inhibiting the binding or association of a 158P3D2 protein with its binding partner or with other proteins. Another class comprises a variety of methods for inhibiting the transcription of a 158P3D2 gene or translation of 158P3D2 mRNA. 
     X.A.) Anti-Cancer Vaccines 
     The invention provides cancer vaccines comprising a 158P3D2-related protein or 158P3D2-related nucleic acid. In view of the expression of 158P3D2, cancer vaccines prevent and/or treat 158P3D2-expressing cancers with minimal or no effects on non-target tissues. The use of a tumor antigen in a vaccine that generates humoral and/or cell-mediated immune responses as anti-cancer therapy is well known in the art and has been employed in prostate cancer using human PSMA and rodent PAP immunogens (Hodge et al., 1995, Int. J. Cancer 63:231-237; Fong et al., 1997, J. Immunol. 159:3113-3117). 
     Such methods can be readily practiced by employing a 158P3D2-related protein, or a 158P3D2-encoding nucleic acid molecule and recombinant vectors capable of expressing and presenting the 158P3D2 immunogen (which typically comprises a number of antibody or T cell epitopes). Skilled artisans understand that a wide variety of vaccine systems for delivery of immunoreactive epitopes are known in the art (see, e.g., Heryln et al., Ann Med 1999 February 31(1):66-78; Maruyama et al., Cancer Immunol Immunother 2000 June 49(3):123-32) Briefly, such methods of generating an immune response (e.g. humoral and/or cell-mediated) in a mammal, comprise the steps of: exposing the mammal&#39;s immune system to an immunoreactive epitope (e.g. an epitope present in a 158P3D2 protein shown in  FIG. 3  or analog or homolog thereof) so that the mammal generates an immune response that is specific for that epitope (e.g. generates antibodies that specifically recognize that epitope). In a preferred method, a 158P3D2 immunogen contains a biological motif, see e.g., Tables VIII-XXI and XXII-XLIX, or a peptide of a size range from 158P3D2 indicated in  FIG. 5 ,  FIG. 6 ,  FIG. 7 ,  FIG. 8 , and  FIG. 9 . 
     The entire 158P3D2 protein, immunogenic regions or epitopes thereof can be combined and delivered by various means. Such vaccine compositions can include, for example, lipopeptides (e.g., Vitiello, A. et al., J. Clin. Invest. 95:341, 1995), peptide compositions encapsulated in poly(DL-lactide-co-glycolide) (“PLG”) microspheres (see, e.g., Eldridge, et al., Molec. Immunol. 28:287-294, 1991: Alonso et al., Vaccine 12:299-306, 1994; Jones et al., Vaccine 13:675-681, 1995), peptide compositions contained in immune stimulating complexes (ISCOMS) (see, e.g., Takahashi et al., Nature 344:873-875, 1990; Hu et al., Clin Exp Immunol. 113:235-243, 1998), multiple antigen peptide systems (MAPs) (see e.g., Tam, J. P., Proc. Natl. Acad. Sci. U.S.A. 85:5409-5413, 1988; Tam, J.P., J. Immunol. Methods 196:17-32, 1996), peptides formulated as multivalent peptides; peptides for use in ballistic delivery systems, typically crystallized peptides, viral delivery vectors (Perkus, M. E. et al., In: Concepts in vaccine development, Kaufmann, S. H. E., ed., p. 379, 1996; Chakrabarti, S. et al., Nature 320:535, 1986; Hu, S. L. et al., Nature 320:537, 1986; Kieny, M.-P. et al., AIDS Bio/Technology 4:790, 1986; Top, F. H. et al., J. Infect. Dis. 124:148, 1971; Chanda, P. K. et al., Virology 175:535, 1990), particles of viral or synthetic origin (e.g., Kofler, N. et al., J. Immunol. Methods. 192:25, 1996; Eldridge, J. H. et al., Sem. Hematol. 30:16, 1993; Falo, L. D., Jr. et al., Nature Med. 7:649, 1995), adjuvants (Warren, H. S., Vogel, F. R., and Chedid, L. A. Annu. Rev. Immunol. 4:369, 1986; Gupta, R. K. et al., Vaccine 11:293, 1993), liposomes (Reddy, R. et al., J. Immunol. 148:1585, 1992; Rock, K. L., Immunol. Today 17:131, 1996), or, naked or particle absorbed cDNA (Ulmer, J. B. et al., Science 259:1745, 1993; Robinson, H. L., Hunt, L. A., and Webster, R. G., Vaccine 11:957, 1993; Shiver, J. W. et al., In: Concepts in vaccine development, Kaufmann, S. H. E., ed., p. 423, 1996; Cease, K. B., and Berzofsky, J. A., Annu. Rev. Immunol. 12:923, 1994 and Eldridge, J. H. et al., Sem. Hematol. 30:16, 1993). Toxin-targeted delivery technologies, also known as receptor mediated targeting, such as those of Avant Immunotherapeutics, Inc. (Needham, Mass.) may also be used. 
     In patients with 158P3D2-associated cancer, the vaccine compositions of the invention can also be used in conjunction with other treatments used for cancer, e.g., surgery, chemotherapy, drug therapies, radiation therapies, etc. including use in combination with immune adjuvants such as IL-2, IL-12, GM-CSF, and the like. 
     X.A.1. Cellular Vaccines 
     CTL epitopes can be determined using specific algorithms to identify peptides within 158P3D2 protein that bind corresponding HLA alleles (see e.g., Table IV; Epimer™ and Epimatrix™, Brown University (URL brown.edu/Research/TB-HIV_Lab/epimatrix/epimatrix.html); and, BIMAS, (URL bimas.dcrt.nih.gov/; SYFPEITHI at URL syfpeithi.bmi-heidelberg.com/). In a preferred embodiment, a 158P3D2 immunogen contains one or more amino acid sequences identified using techniques well known in the art, such as the sequences shown in Tables VIII-XXI and XXII-XLIX or a peptide of 8, 9, 10 or 11 amino acids specified by an HLA Class I motif/supermotif (e.g., Table IV (A), Table IV (D), or Table IV (E)) and/or a peptide of at least 9 amino acids that comprises an HLA Class II motif/supermotif (e.g., Table IV (B) or Table IV (C)). As is appreciated in the art, the HLA Class I binding groove is essentially closed ended so that peptides of only a particular size range can fit into the groove and be bound, generally HLA Class I epitopes are 8, 9, 10, or 11 amino acids long. In contrast, the HLA Class II binding groove is essentially open ended; therefore a peptide of about 9 or more amino acids can be bound by an HLA Class II molecule. Due to the binding groove differences between HLA Class I and II, HLA Class I motifs are length specific, i.e., position two of a Class I motif is the second amino acid in an amino to carboxyl direction of the peptide. The amino acid positions in a Class II motif are relative only to each other, not the overall peptide, i.e., additional amino acids can be attached to the amino and/or carboxyl termini of a motif-bearing sequence. HLA Class II epitopes are often 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 amino acids long, or longer than 25 amino acids. 
     X.A.2. Antibody-Based Vaccines 
     A wide variety of methods for generating an immune response in a mammal are known in the art (for example as the first step in the generation of hybridomas). Methods of generating an immune response in a mammal comprise exposing the mammal&#39;s immune system to an immunogenic epitope on a protein (e.g. a 158P3D2 protein) so that an immune response is generated. A typical embodiment consists of a method for generating an immune response to 158P3D2 in a host, by contacting the host with a sufficient amount of at least one 158P3D2 B cell or cytotoxic T-cell epitope or analog thereof; and at least one periodic interval thereafter re-contacting the host with the 158P3D2 B cell or cytotoxic T-cell epitope or analog thereof. A specific embodiment consists of a method of generating an immune response against a 158P3D2-related protein or a man-made multiepitopic peptide comprising: administering 158P3D2 immunogen (e.g. a 158P3D2 protein or a peptide fragment thereof, a 158P3D2 fusion protein or analog etc.) in a vaccine preparation to a human or another mammal Typically, such vaccine preparations further contain a suitable adjuvant (see, e.g., U.S. Pat. No. 6,146,635) or a universal helper epitope such as a PADRE™ peptide (Epimmune Inc., San Diego, Calif.; see, e.g., Alexander et al., J. Immunol. 2000 164(3); 164(3): 1625-1633; Alexander et al., Immunity 1994 1(9): 751-761 and Alexander et al., Immunol. Res. 1998 18(2): 79-92). An alternative method comprises generating an immune response in an individual against a 158P3D2 immunogen by: administering in vivo to muscle or skin of the individual&#39;s body a DNA molecule that comprises a DNA sequence that encodes a 158P3D2 immunogen, the DNA sequence operatively linked to regulatory sequences which control the expression of the DNA sequence; wherein the DNA molecule is taken up by cells, the DNA sequence is expressed in the cells and an immune response is generated against the immunogen (see, e.g., U.S. Pat. No. 5,962,428). Optionally a genetic vaccine facilitator such as anionic lipids; saponins; lectins; estrogenic compounds; hydroxylated lower alkyls; dimethyl sulfoxide; and urea is also administered. In addition, an antiidiotypic antibody can be administered that mimics 158P3D2, in order to generate a response to the target antigen. 
     X.A.3. Nucleic Acid Vaccines: 
     Vaccine compositions of the invention include nucleic acid-mediated modalities. DNA or RNA that encode protein(s) of the invention can be administered to a patient. Genetic immunization methods can be employed to generate prophylactic or therapeutic humoral and cellular immune responses directed against cancer cells expressing 158P3D2. Constructs comprising DNA encoding a 158P3D2-related protein/immunogen and appropriate regulatory sequences can be injected directly into muscle or skin of an individual, such that the cells of the muscle or skin take-up the construct and express the encoded 158P3D2 protein/immunogen. Alternatively, a vaccine comprises a 158P3D2-related protein. Expression of the 158P3D2-related protein immunogen results in the generation of prophylactic or therapeutic humoral and cellular immunity against cells that bear a 158P3D2 protein. Various prophylactic and therapeutic genetic immunization techniques known in the art can be used (for review, see information and references published at Internet address genweb.com). Nucleic acid-based delivery is described, for instance, in Wolff et. al., Science 247:1465 (1990) as well as U.S. Pat. Nos. 5,580,859; 5,589,466; 5,804,566; 5,739,118; 5,736,524; 5,679,647; WO 98/04720. Examples of DNA-based delivery technologies include “naked DNA”, facilitated (bupivicaine, polymers, peptide-mediated) delivery, cationic lipid complexes, and particle-mediated (“gene gun”) or pressure-mediated delivery (see, e.g., U.S. Pat. No. 5,922,687). 
     For therapeutic or prophylactic immunization purposes, proteins of the invention can be expressed via viral or bacterial vectors. Various viral gene delivery systems that can be used in the practice of the invention include, but are not limited to, vaccinia, fowlpox, canarypox, adenovirus, influenza, poliovirus, adeno-associated virus, lentivirus, and sindbis virus (see, e.g., Restifo, 1996, Curr. Opin. Immunol. 8:658-663; Tsang et al. J. Natl. Cancer Inst. 87:982-990 (1995)). Non-viral delivery systems can also be employed by introducing naked DNA encoding a 158P3D2-related protein into the patient (e.g., intramuscularly or intradermally) to induce an anti-tumor response. 
     Vaccinia virus is used, for example, as a vector to express nucleotide sequences that encode the peptides of the invention. Upon introduction into a host, the recombinant vaccinia virus expresses the protein immunogenic peptide, and thereby elicits a host immune response. Vaccinia vectors and methods useful in immunization protocols are described in, e.g., U.S. Pat. No. 4,722,848. Another vector is BCG (Bacille Calmette Guerin). BCG vectors are described in Stover et al., Nature 351:456-460 (1991). A wide variety of other vectors useful for therapeutic administration or immunization of the peptides of the invention, e.g. adeno and adeno-associated virus vectors, retroviral vectors,  Salmonella typhi  vectors, detoxified anthrax toxin vectors, and the like, will be apparent to those skilled in the art from the description herein. 
     Thus, gene delivery systems are used to deliver a 158P3D2-related nucleic acid molecule. In one embodiment, the full-length human 158P3D2 cDNA is employed. In another embodiment, 158P3D2 nucleic acid molecules encoding specific cytotoxic T lymphocyte (CTL) and/or antibody epitopes are employed. 
     X.A.4. Ex Vivo Vaccines 
     Various ex vivo strategies can also be employed to generate an immune response. One approach involves the use of antigen presenting cells (APCs) such as dendritic cells (DC) to present 158P3D2 antigen to a patient&#39;s immune system. Dendritic cells express MHC class I and II molecules, B7 co-stimulator, and IL-12, and are thus highly specialized antigen presenting cells. In prostate cancer, autologous dendritic cells pulsed with peptides of the prostate-specific membrane antigen (PSMA) are being used in a Phase I clinical trial to stimulate prostate cancer patients&#39; immune systems (Tjoa et al., 1996, Prostate 28:65-69; Murphy et al., 1996, Prostate 29:371-380). Thus, dendritic cells can be used to present 158P3D2 peptides to T cells in the context of MHC class I or II molecules. In one embodiment, autologous dendritic cells are pulsed with 158P3D2 peptides capable of binding to MHC class I and/or class II molecules. In another embodiment, dendritic cells are pulsed with the complete 158P3D2 protein. Yet another embodiment involves engineering the overexpression of a 158P3D2 gene in dendritic cells using various implementing vectors known in the art, such as adenovirus (Arthur et al., 1997, Cancer Gene Ther. 4:17-25), retrovirus (Henderson et al., 1996, Cancer Res. 56:3763-3770), lentivirus, adeno-associated virus, DNA transfection (Ribas et al., 1997, Cancer Res. 57:2865-2869), or tumor-derived RNA transfection (Ashley et al., 1997, J. Exp. Med. 186:1177-1182). Cells that express 158P3D2 can also be engineered to express immune modulators, such as GM-CSF, and used as immunizing agents. 
     X.B.) 158P3D2 as a Target for Antibody-Based Therapy 
     158P3D2 is an attractive target for antibody-based therapeutic strategies. A number of antibody strategies are known in the art for targeting both extracellular and intracellular molecules (see, e.g., complement and ADCC mediated killing as well as the use of intrabodies). Because 158P3D2 is expressed by cancer cells of various lineages relative to corresponding normal cells, systemic administration of 158P3D2-immunoreactive compositions are prepared that exhibit excellent sensitivity without toxic, non-specific and/or non-target effects caused by binding of the immunoreactive composition to non-target organs and tissues. Antibodies specifically reactive with domains of 158P3D2 are useful to treat 158P3D2-expressing cancers systemically, either as conjugates with a toxin or therapeutic agent, or as naked antibodies capable of inhibiting cell proliferation or function. 
     158P3D2 antibodies can be introduced into a patient such that the antibody binds to 158P3D2 and modulates a function, such as an interaction with a binding partner, and consequently mediates destruction of the tumor cells and/or inhibits the growth of the tumor cells. Mechanisms by which such antibodies exert a therapeutic effect can include complement-mediated cytolysis, antibody-dependent cellular cytotoxicity, modulation of the physiological function of 158P3D2, inhibition of ligand binding or signal transduction pathways, modulation of tumor cell differentiation, alteration of tumor angiogenesis factor profiles, and/or apoptosis. 
     Those skilled in the art understand that antibodies can be used to specifically target and bind immunogenic molecules such as an immunogenic region of a 158P3D2 sequence shown in  FIG. 2  or  FIG. 3 . In addition, skilled artisans understand that it is routine to conjugate antibodies to cytotoxic agents (see, e.g., Sievers et al. Blood 93:11 3678-3684 (Jun. 1, 1999)). When cytotoxic and/or therapeutic agents are delivered directly to cells, such as by conjugating them to antibodies specific for a molecule expressed by that cell (e.g. 158P3D2), the cytotoxic agent will exert its known biological effect (i.e. cytotoxicity) on those cells. 
     A wide variety of compositions and methods for using antibody-cytotoxic agent conjugates to kill cells are known in the art. In the context of cancers, typical methods entail administering to an animal having a tumor a biologically effective amount of a conjugate comprising a selected cytotoxic and/or therapeutic agent linked to a targeting agent (e.g. an anti-158P3D2 antibody) that binds to a marker (e.g. 158P3D2) expressed, accessible to binding or localized on the cell surfaces. A typical embodiment is a method of delivering a cytotoxic and/or therapeutic agent to a cell expressing 158P3D2, comprising conjugating the cytotoxic agent to an antibody that immunospecifically binds to a 158P3D2 epitope, and, exposing the cell to the antibody-agent conjugate. Another illustrative embodiment is a method of treating an individual suspected of suffering from metastasized cancer, comprising a step of administering parenterally to said individual a pharmaceutical composition comprising a therapeutically effective amount of an antibody conjugated to a cytotoxic and/or therapeutic agent. 
     Cancer immunotherapy using anti-158P3D2 antibodies can be done in accordance with various approaches that have been successfully employed in the treatment of other types of cancer, including but not limited to colon cancer (Arlen et al., 1998, Crit. Rev. Immunol. 18:133-138), multiple myeloma (Ozaki et al., 1997, Blood 90:3179-3186, Tsunenari et al., 1997, Blood 90:2437-2444), gastric cancer (Kasprzyk et al., 1992, Cancer Res. 52:2771-2776), B-cell lymphoma (Funakoshi et al., 1996, J. Immunother. Emphasis Tumor Immunol. 19:93-101), leukemia (Zhong et al., 1996, Leuk. Res. 20:581-589), colorectal cancer (Moun et al., 1994, Cancer Res. 54:6160-6166; Velders et al., 1995, Cancer Res. 55:4398-4403), and breast cancer (Shepard et al., 1991, J. Clin. Immunol. 11:117-127). Some therapeutic approaches involve conjugation of naked antibody to a toxin or radioisotope, such as the conjugation of Y91 or I131 to anti-CD20 antibodies (e.g., Zevalin™, IDEC Pharmaceuticals Corp. or Bexxar™, Coulter Pharmaceuticals), while others involve co-administration of antibodies and other therapeutic agents, such as Herceptin™ (trastuzumab) with paclitaxel (Genentech, Inc.). The antibodies can be conjugated to a therapeutic agent. To treat prostate cancer, for example, 158P3D2 antibodies can be administered in conjunction with radiation, chemotherapy or hormone ablation. Also, antibodies can be conjugated to a toxin such as calicheamicin (e.g., Mylotarg™ Wyeth-Ayerst, Madison, N.J., a recombinant humanized IgG4 kappa antibody conjugated to antitumor antibiotic calicheamicin) or a maytansinoid (e.g., taxane-based Tumor-Activated Prodrug, TAP, platform, ImmunoGen, Cambridge, Mass., also see e.g., U.S. Pat. No. 5,416,064). 
     Although 158P3D2 antibody therapy is useful for all stages of cancer, antibody therapy can be particularly appropriate in advanced or metastatic cancers. Treatment with the antibody therapy of the invention is indicated for patients who have received one or more rounds of chemotherapy. Alternatively, antibody therapy of the invention is combined with a chemotherapeutic or radiation regimen for patients who have not received chemotherapeutic treatment. Additionally, antibody therapy can enable the use of reduced dosages of concomitant chemotherapy, particularly for patients who do not tolerate the toxicity of the chemotherapeutic agent very well. Fan et al. (Cancer Res. 53:4637-4642, 1993), Prewett et al. (International J. of Onco. 9:217-224, 1996), and Hancock et al. (Cancer Res. 51:4575-4580, 1991) describe the use of various antibodies together with chemotherapeutic agents. 
     Although 158P3D2 antibody therapy is useful for all stages of cancer, antibody therapy can be particularly appropriate in advanced or metastatic cancers. Treatment with the antibody therapy of the invention is indicated for patients who have received one or more rounds of chemotherapy. Alternatively, antibody therapy of the invention is combined with a chemotherapeutic or radiation regimen for patients who have not received chemotherapeutic treatment. Additionally, antibody therapy can enable the use of reduced dosages of concomitant chemotherapy, particularly for patients who do not tolerate the toxicity of the chemotherapeutic agent very well. 
     Cancer patients can be evaluated for the presence and level of 158P3D2 expression, preferably using immunohistochemical assessments of tumor tissue, quantitative 158P3D2 imaging, or other techniques that reliably indicate the presence and degree of 158P3D2 expression Immunohistochemical analysis of tumor biopsies or surgical specimens is preferred for this purpose. Methods for immunohistochemical analysis of tumor tissues are well known in the art. 
     Anti-158P3D2 monoclonal antibodies that treat prostate and other cancers include those that initiate a potent immune response against the tumor or those that are directly cytotoxic. In this regard, anti-158P3D2 monoclonal antibodies (mAbs) can elicit tumor cell lysis by either complement-mediated or antibody-dependent cell cytotoxicity (ADCC) mechanisms, both of which require an intact Fc portion of the immunoglobulin molecule for interaction with effector cell Fc receptor sites on complement proteins. In addition, anti-158P3D2 mAbs that exert a direct biological effect on tumor growth are useful to treat cancers that express 158P3D2. Mechanisms by which directly cytotoxic mAbs act include: inhibition of cell growth, modulation of cellular differentiation, modulation of tumor angiogenesis factor profiles, and the induction of apoptosis. The mechanism(s) by which a particular anti-158P3D2 mAb exerts an anti-tumor effect is evaluated using any number of in vitro assays that evaluate cell death such as ADCC, ADMMC, complement-mediated cell lysis, and so forth, as is generally known in the art. 
     In some patients, the use of murine or other non-human monoclonal antibodies, or human/mouse chimeric mAbs can induce moderate to strong immune responses against the non-human antibody. This can result in clearance of the antibody from circulation and reduced efficacy. In the most severe cases, such an immune response can lead to the extensive formation of immune complexes which, potentially, can cause renal failure. Accordingly, preferred monoclonal antibodies used in the therapeutic methods of the invention are those that are either fully human or humanized and that bind specifically to the target 158P3D2 antigen with high affinity but exhibit low or no antigenicity in the patient. 
     Therapeutic methods of the invention contemplate the administration of single anti-158P3D2 mAbs as well as combinations, or cocktails, of different mAbs. Such mAb cocktails can have certain advantages inasmuch as they contain mAbs that target different epitopes, exploit different effector mechanisms or combine directly cytotoxic mAbs with mAbs that rely on immune effector functionality. Such mAbs in combination can exhibit synergistic therapeutic effects. In addition, anti-158P3D2 mAbs can be administered concomitantly with other therapeutic modalities, including but not limited to various chemotherapeutic agents, androgen-blockers, immune modulators (e.g., IL-2, GM-CSF), surgery or radiation. The anti-158P3D2 mAbs are administered in their “naked” or unconjugated form, or can have a therapeutic agent(s) conjugated to them. 
     Anti-158P3D2 antibody formulations are administered via any route capable of delivering the antibodies to a tumor cell. Routes of administration include, but are not limited to, intravenous, intraperitoneal, intramuscular, intratumor, intradermal, and the like. Treatment generally involves repeated administration of the anti-158P3D2 antibody preparation, via an acceptable route of administration such as intravenous injection (IV), typically at a dose in the range of about 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, or 25 mg/kg body weight. In general, doses in the range of 10-1000 mg mAb per week are effective and well tolerated. 
     Based on clinical experience with the Herceptin™ mAb in the treatment of metastatic breast cancer, an initial loading dose of approximately 4 mg/kg patient body weight IV, followed by weekly doses of about 2 mg/kg IV of the anti-158P3D2 mAb preparation represents an acceptable dosing regimen. Preferably, the initial loading dose is administered as a 90-minute or longer infusion. The periodic maintenance dose is administered as a 30 minute or longer infusion, provided the initial dose was well tolerated. As appreciated by those of skill in the art, various factors can influence the ideal dose regimen in a particular case. Such factors include, for example, the binding affinity and half life of the Ab or mAbs used, the degree of 158P3D2 expression in the patient, the extent of circulating shed 158P3D2 antigen, the desired steady-state antibody concentration level, frequency of treatment, and the influence of chemotherapeutic or other agents used in combination with the treatment method of the invention, as well as the health status of a particular patient. 
     Optionally, patients should be evaluated for the levels of 158P3D2 in a given sample (e.g. the levels of circulating 158P3D2 antigen and/or 158P3D2 expressing cells) in order to assist in the determination of the most effective dosing regimen, etc. Such evaluations are also used for monitoring purposes throughout therapy, and are useful to gauge therapeutic success in combination with the evaluation of other parameters (for example, urine cytology and/or ImmunoCyt levels in bladder cancer therapy, or by analogy, serum PSA levels in prostate cancer therapy). 
     Anti-idiotypic anti-158P3D2 antibodies can also be used in anti-cancer therapy as a vaccine for inducing an immune response to cells expressing a 158P3D2-related protein. In particular, the generation of anti-idiotypic antibodies is well known in the art; this methodology can readily be adapted to generate anti-idiotypic anti-158P3D2 antibodies that mimic an epitope on a 158P3D2-related protein (see, for example, Wagner et al., 1997, Hybridoma 16: 33-40; Foon et al., 1995, J. Clin. Invest. 96:334-342; Herlyn et al., 1996, Cancer Immunol. Immunother. 43:65-76). Such an anti-idiotypic antibody can be used in cancer vaccine strategies. 
     X.C.) 158P3D2 as a Target for Cellular Immune Responses 
     Vaccines and methods of preparing vaccines that contain an immunogenically effective amount of one or more HLA-binding peptides as described herein are further embodiments of the invention. Furthermore, vaccines in accordance with the invention encompass compositions of one or more of the claimed peptides. A peptide can be present in a vaccine individually. Alternatively, the peptide can exist as a homopolymer comprising multiple copies of the same peptide, or as a heteropolymer of various peptides. Polymers have the advantage of increased immunological reaction and, where different peptide epitopes are used to make up the polymer, the additional ability to induce antibodies and/or CTLs that react with different antigenic determinants of the pathogenic organism or tumor-related peptide targeted for an immune response. The composition can be a naturally occurring region of an antigen or can be prepared, e.g., recombinantly or by chemical synthesis. 
     Carriers that can be used with vaccines of the invention are well known in the art, and include, e.g., thyroglobulin, albumins such as human serum albumin, tetanus toxoid, polyamino acids such as poly 1-lysine, poly 1-glutamic acid, influenza, hepatitis B virus core protein, and the like. The vaccines can contain a physiologically tolerable (i.e., acceptable) diluent such as water, or saline, preferably phosphate buffered saline. The vaccines also typically include an adjuvant. Adjuvants such as incomplete Freund&#39;s adjuvant, aluminum phosphate, aluminum hydroxide, or alum are examples of materials well known in the art. Additionally, as disclosed herein, CTL responses can be primed by conjugating peptides of the invention to lipids, such as tripalmitoyl-S-glycerylcysteinlyseryl-serine (P3CSS). Moreover, an adjuvant such as a synthetic cytosine-phosphorothiolated-guanine-containing (CpG) oligonucleotides has been found to increase CTL responses 10- to 100-fold. (see, e.g. Davila and Celis, J. Immunol. 165:539-547 (2000)). 
     Upon immunization with a peptide composition in accordance with the invention, via injection, aerosol, oral, transdermal, transmucosal, intrapleural, intrathecal, or other suitable routes, the immune system of the host responds to the vaccine by producing large amounts of CTLs and/or HTLs specific for the desired antigen. Consequently, the host becomes at least partially immune to later development of cells that express or overexpress 158P3D2 antigen, or derives at least some therapeutic benefit when the antigen was tumor-associated. 
     In some embodiments, it may be desirable to combine the class I peptide components with components that induce or facilitate neutralizing antibody and or helper T cell responses directed to the target antigen. A preferred embodiment of such a composition comprises class I and class II epitopes in accordance with the invention. An alternative embodiment of such a composition comprises a class I and/or class II epitope in accordance with the invention, along with a cross reactive HTL epitope such as PADRE™ (Epimmune, San Diego, Calif.) molecule (described e.g., in U.S. Pat. No. 5,736,142). 
     A vaccine of the invention can also include antigen-presenting cells (APC), such as dendritic cells (DC), as a vehicle to present peptides of the invention. Vaccine compositions can be created in vitro, following dendritic cell mobilization and harvesting, whereby loading of dendritic cells occurs in vitro. For example, dendritic cells are transfected, e.g., with a minigene in accordance with the invention, or are pulsed with peptides. The dendritic cell can then be administered to a patient to elicit immune responses in vivo. Vaccine compositions, either DNA- or peptide-based, can also be administered in vivo in combination with dendritic cell mobilization whereby loading of dendritic cells occurs in vivo. 
     Preferably, the following principles are utilized when selecting an array of epitopes for inclusion in a polyepitopic composition for use in a vaccine, or for selecting discrete epitopes to be included in a vaccine and/or to be encoded by nucleic acids such as a minigene. It is preferred that each of the following principles be balanced in order to make the selection. The multiple epitopes to be incorporated in a given vaccine composition may be, but need not be, contiguous in sequence in the native antigen from which the epitopes are derived. 
     1.) Epitopes are selected which, upon administration, mimic immune responses that have been observed to be correlated with tumor clearance. For HLA Class I this includes 3-4 epitopes that come from at least one tumor associated antigen (TAA). For HLA Class II a similar rationale is employed; again 3-4 epitopes are selected from at least one TAA (see, e.g., Rosenberg et al., Science 278:1447-1450). Epitopes from one TAA may be used in combination with epitopes from one or more additional TAAs to produce a vaccine that targets tumors with varying expression patterns of frequently-expressed TAAs. 
     2.) Epitopes are selected that have the requisite binding affinity established to be correlated with immunogenicity: for HLA Class I an IC50 of 500 nM or less, often 200 nM or less; and for Class II an IC50 of 1000 nM or less. 
     3.) Sufficient supermotif bearing-peptides, or a sufficient array of allele-specific motif-bearing peptides, are selected to give broad population coverage. For example, it is preferable to have at least 80% population coverage. A Monte Carlo analysis, a statistical evaluation known in the art, can be employed to assess the breadth, or redundancy of, population coverage. 
     4.) When selecting epitopes from cancer-related antigens it is often useful to select analogs because the patient may have developed tolerance to the native epitope. 
     5.) Of particular relevance are epitopes referred to as “nested epitopes.” Nested epitopes occur where at least two epitopes overlap in a given peptide sequence. A nested peptide sequence can comprise B cell, HLA class I and/or HLA class II epitopes. When providing nested epitopes, a general objective is to provide the greatest number of epitopes per sequence. Thus, an aspect is to avoid providing a peptide that is any longer than the amino terminus of the amino terminal epitope and the carboxyl terminus of the carboxyl terminal epitope in the peptide. When providing a multi-epitopic sequence, such as a sequence comprising nested epitopes, it is generally important to screen the sequence in order to insure that it does not have pathological or other deleterious biological properties. 
     6.) If a polyepitopic protein is created, or when creating a minigene, an objective is to generate the smallest peptide that encompasses the epitopes of interest. This principle is similar, if not the same as that employed when selecting a peptide comprising nested epitopes. However, with an artificial polyepitopic peptide, the size minimization objective is balanced against the need to integrate any spacer sequences between epitopes in the polyepitopic protein. Spacer amino acid residues can, for example, be introduced to avoid junctional epitopes (an epitope recognized by the immune system, not present in the target antigen, and only created by the man-made juxtaposition of epitopes), or to facilitate cleavage between epitopes and thereby enhance epitope presentation. Junctional epitopes are generally to be avoided because the recipient may generate an immune response to that non-native epitope. Of particular concern is a junctional epitope that is a “dominant epitope.” A dominant epitope may lead to such a zealous response that immune responses to other epitopes are diminished or suppressed. 
     7.) Where the sequences of multiple variants of the same target protein are present, potential peptide epitopes can also be selected on the basis of their conservancy. For example, a criterion for conservancy may define that the entire sequence of an HLA class I binding peptide or the entire 9-mer core of a class II binding peptide be conserved in a designated percentage of the sequences evaluated for a specific protein antigen. 
     X.C.1. Minigene Vaccines 
     A number of different approaches are available which allow simultaneous delivery of multiple epitopes. Nucleic acids encoding the peptides of the invention are a particularly useful embodiment of the invention. Epitopes for inclusion in a minigene are preferably selected according to the guidelines set forth in the previous section. A preferred means of administering nucleic acids encoding the peptides of the invention uses minigene constructs encoding a peptide comprising one or multiple epitopes of the invention. 
     The use of multi-epitope minigenes is described below and in, Ishioka et al., J. Immunol. 162:3915-3925, 1999; An, L. and Whitton, J. L., J. Virol. 71:2292, 1997; Thomson, S. A. et al., J. Immunol. 157:822, 1996; Whitton, J. L. et al., J. Virol. 67:348, 1993; Hanke, R. et al., Vaccine 16:426, 1998. For example, a multi-epitope DNA plasmid encoding supermotif- and/or motif-bearing epitopes derived 158P3D2, the PADRE® universal helper T cell epitope or multiple HTL epitopes from 158P3D2 (see e.g., Tables VIII-XXI and XXII to XLIX), and an endoplasmic reticulum-translocating signal sequence can be engineered. A vaccine may also comprise epitopes that are derived from other TAAs. 
     The immunogenicity of a multi-epitopic minigene can be confirmed in transgenic mice to evaluate the magnitude of CTL induction responses against the epitopes tested. Further, the immunogenicity of DNA-encoded epitopes in vivo can be correlated with the in vitro responses of specific CTL lines against target cells transfected with the DNA plasmid. Thus, these experiments can show that the minigene serves to both: 1.) generate a CTL response and 2.) that the induced CTLs recognized cells expressing the encoded epitopes. 
     For example, to create a DNA sequence encoding the selected epitopes (minigene) for expression in human cells, the amino acid sequences of the epitopes may be reverse translated. A human codon usage table can be used to guide the codon choice for each amino acid. These epitope-encoding DNA sequences may be directly adjoined, so that when translated, a continuous polypeptide sequence is created. To optimize expression and/or immunogenicity, additional elements can be incorporated into the minigene design. Examples of amino acid sequences that can be reverse translated and included in the minigene sequence include: HLA class I epitopes, HLA class II epitopes, antibody epitopes, a ubiquitination signal sequence, and/or an endoplasmic reticulum targeting signal. In addition, HLA presentation of CTL and HTL epitopes may be improved by including synthetic (e.g. poly-alanine) or naturally-occurring flanking sequences adjacent to the CTL or HTL epitopes; these larger peptides comprising the epitope(s) are within the scope of the invention. 
     The minigene sequence may be converted to DNA by assembling oligonucleotides that encode the plus and minus strands of the minigene. Overlapping oligonucleotides (30-100 bases long) may be synthesized, phosphorylated, purified and annealed under appropriate conditions using well known techniques. The ends of the oligonucleotides can be joined, for example, using T4 DNA ligase. This synthetic minigene, encoding the epitope polypeptide, can then be cloned into a desired expression vector. 
     Standard regulatory sequences well known to those of skill in the art are preferably included in the vector to ensure expression in the target cells. Several vector elements are desirable: a promoter with a down-stream cloning site for minigene insertion; a polyadenylation signal for efficient transcription termination; an  E. coli  origin of replication; and an  E. coli  selectable marker (e.g. ampicillin or kanamycin resistance). Numerous promoters can be used for this purpose, e.g., the human cytomegalovirus (hCMV) promoter. See, e.g., U.S. Pat. Nos. 5,580,859 and 5,589,466 for other suitable promoter sequences. 
     Additional vector modifications may be desired to optimize minigene expression and immunogenicity. In some cases, introns are required for efficient gene expression, and one or more synthetic or naturally-occurring introns could be incorporated into the transcribed region of the minigene. The inclusion of mRNA stabilization sequences and sequences for replication in mammalian cells may also be considered for increasing minigene expression. 
     Once an expression vector is selected, the minigene is cloned into the polylinker region downstream of the promoter. This plasmid is transformed into an appropriate  E. coli  strain, and DNA is prepared using standard techniques. The orientation and DNA sequence of the minigene, as well as all other elements included in the vector, are confirmed using restriction mapping and DNA sequence analysis. Bacterial cells harboring the correct plasmid can be stored as a master cell bank and a working cell bank. 
     In addition, immunostimulatory sequences (ISSs or CpGs) appear to play a role in the immunogenicity of DNA vaccines. These sequences may be included in the vector, outside the minigene coding sequence, if desired to enhance immunogenicity. 
     In some embodiments, a bi-cistronic expression vector which allows production of both the minigene-encoded epitopes and a second protein (included to enhance or decrease immunogenicity) can be used. Examples of proteins or polypeptides that could beneficially enhance the immune response if co-expressed include cytokines (e.g., IL-2, IL-12, GM-CSF), cytokine-inducing molecules (e.g., LeIF), costimulatory molecules, or for HTL responses, pan-DR binding proteins (PADRE™, Epimmune, San Diego, Calif.). Helper (HTL) epitopes can be joined to intracellular targeting signals and expressed separately from expressed CTL epitopes; this allows direction of the HTL epitopes to a cell compartment different than that of the CTL epitopes. If required, this could facilitate more efficient entry of HTL epitopes into the HLA class II pathway, thereby improving HTL induction. In contrast to HTL or CTL induction, specifically decreasing the immune response by co-expression of immunosuppressive molecules (e.g. TGF-β) may be beneficial in certain diseases. 
     Therapeutic quantities of plasmid DNA can be produced for example, by fermentation in  E. coli , followed by purification. Aliquots from the working cell bank are used to inoculate growth medium, and grown to saturation in shaker flasks or a bioreactor according to well-known techniques. Plasmid DNA can be purified using standard bioseparation technologies such as solid phase anion-exchange resins supplied by QIAGEN, Inc. (Valencia, Calif.). If required, supercoiled DNA can be isolated from the open circular and linear forms using gel electrophoresis or other methods. 
     Purified plasmid DNA can be prepared for injection using a variety of formulations. The simplest of these is reconstitution of lyophilized DNA in sterile phosphate-buffer saline (PBS). This approach, known as “naked DNA,” is currently being used for intramuscular (IM) administration in clinical trials. To maximize the immunotherapeutic effects of minigene DNA vaccines, an alternative method for formulating purified plasmid DNA may be desirable. A variety of methods have been described, and new techniques may become available. Cationic lipids, glycolipids, and fusogenic liposomes can also be used in the formulation (see, e.g., as described by WO 93/24640; Mannino &amp; Gould-Fogerite, BioTechniques 6(7): 682 (1988); U.S. Pat. No. 5,279,833; WO 91/06309; and Feigner, et al., Proc. Nat&#39;l Acad. Sci. USA 84:7413 (1987). In addition, peptides and compounds referred to collectively as protective, interactive, non-condensing compounds (PINC) could also be complexed to purified plasmid DNA to influence variables such as stability, intramuscular dispersion, or trafficking to specific organs or cell types. 
     Target cell sensitization can be used as a functional assay for expression and HLA class I presentation of minigene-encoded CTL epitopes. For example, the plasmid DNA is introduced into a mammalian cell line that is suitable as a target for standard CTL chromium release assays. The transfection method used will be dependent on the final formulation. Electroporation can be used for “naked” DNA, whereas cationic lipids allow direct in vitro transfection. A plasmid expressing green fluorescent protein (GFP) can be co-transfected to allow enrichment of transfected cells using fluorescence activated cell sorting (FACS). These cells are then chromium-51 (51Cr) labeled and used as target cells for epitope-specific CTL lines; cytolysis, detected by 51Cr release, indicates both production of, and HLA presentation of, minigene-encoded CTL epitopes. Expression of HTL epitopes may be evaluated in an analogous manner using assays to assess HTL activity. 
     In vivo immunogenicity is a second approach for functional testing of minigene DNA formulations. Transgenic mice expressing appropriate human HLA proteins are immunized with the DNA product. The dose and route of administration are formulation dependent (e.g., IM for DNA in PBS, intraperitoneal (i.p.) for lipid-complexed DNA). Twenty-one days after immunization, splenocytes are harvested and restimulated for one week in the presence of peptides encoding each epitope being tested. Thereafter, for CTL effector cells, assays are conducted for cytolysis of peptide-loaded, 51Cr-labeled target cells using standard techniques. Lysis of target cells that were sensitized by HLA loaded with peptide epitopes, corresponding to minigene-encoded epitopes, demonstrates DNA vaccine function for in vivo induction of CTLs Immunogenicity of HTL epitopes is confirmed in transgenic mice in an analogous manner. 
     Alternatively, the nucleic acids can be administered using ballistic delivery as described, for instance, in U.S. Pat. No. 5,204,253. Using this technique, particles comprised solely of DNA are administered. In a further alternative embodiment, DNA can be adhered to particles, such as gold particles. 
     Minigenes can also be delivered using other bacterial or viral delivery systems well known in the art, e.g., an expression construct encoding epitopes of the invention can be incorporated into a viral vector such as vaccinia. 
     X.C.2. Combinations of CTL Peptides with Helper Peptides 
     Vaccine compositions comprising CTL peptides of the invention can be modified, e.g., analoged, to provide desired attributes, such as improved serum half life, broadened population coverage or enhanced immunogenicity. 
     For instance, the ability of a peptide to induce CTL activity can be enhanced by linking the peptide to a sequence which contains at least one epitope that is capable of inducing a T helper cell response. Although a CTL peptide can be directly linked to a T helper peptide, often CTL epitope/HTL epitope conjugates are linked by a spacer molecule. The spacer is typically comprised of relatively small, neutral molecules, such as amino acids or amino acid mimetics, which are substantially uncharged under physiological conditions. The spacers are typically selected from, e.g., Ala, Gly, or other neutral spacers of nonpolar amino acids or neutral polar amino acids. It will be understood that the optionally present spacer need not be comprised of the same residues and thus may be a hetero- or homo-oligomer. When present, the spacer will usually be at least one or two residues, more usually three to six residues and sometimes 10 or more residues. The CTL peptide epitope can be linked to the T helper peptide epitope either directly or via a spacer either at the amino or carboxy terminus of the CTL peptide. The amino terminus of either the immunogenic peptide or the T helper peptide may be acylated. 
     In certain embodiments, the T helper peptide is one that is recognized by T helper cells present in a majority of a genetically diverse population. This can be accomplished by selecting peptides that bind to many, most, or all of the HLA class II molecules. Examples of such amino acid bind many HLA Class II molecules include sequences from antigens such as tetanus toxoid at positions 830-843 QYIKANSKFIGITE; (SEQ ID NO:63),  Plasmodium falciparum  circumsporozoite (CS) protein at positions 378-398 DIEKKIAKMEKASSVFNVVNS; (SEQ ID NO:64), and  Streptococcus  18 kD protein at positions 116-131 GAVDSILGGVATYGAA; (SEQ ID NO:65). Other examples include peptides bearing a DR 1-4-7 supermotif, or either of the DR3 motifs. 
     Alternatively, it is possible to prepare synthetic peptides capable of stimulating T helper lymphocytes, in a loosely HLA-restricted fashion, using amino acid sequences not found in nature (see, e.g., PCT publication WO 95/07707). These synthetic compounds called Pan-DR-binding epitopes (e.g., PADRE™, Epimmune, Inc., San Diego, Calif.) are designed, most preferably, to bind most HLA-DR (human HLA class II) molecules. For instance, a pan-DR-binding epitope peptide having the formula: xKXVAAWTLKAAx (SEQ ID NO:66), where “X” is either cyclohexylalanine, phenylalanine, or tyrosine, and a is either d-alanine or 1-alanine, has been found to bind to most HLA-DR alleles, and to stimulate the response of T helper lymphocytes from most individuals, regardless of their HLA type. An alternative of a pan-DR binding epitope comprises all “L” natural amino acids and can be provided in the form of nucleic acids that encode the epitope. 
     HTL peptide epitopes can also be modified to alter their biological properties. For example, they can be modified to include d-amino acids to increase their resistance to proteases and thus extend their serum half life, or they can be conjugated to other molecules such as lipids, proteins, carbohydrates, and the like to increase their biological activity. For example, a T helper peptide can be conjugated to one or more palmitic acid chains at either the amino or carboxyl termini. 
     X.C.3. Combinations of CTL Peptides with T Cell Priming Agents 
     In some embodiments it may be desirable to include in the pharmaceutical compositions of the invention at least one component which primes B lymphocytes or T lymphocytes. Lipids have been identified as agents capable of priming CTL in vivo. For example, palmitic acid residues can be attached to the ε- and α-amino groups of a lysine residue and then linked, e.g., via one or more linking residues such as Gly, Gly-Gly-, Ser, Ser-Ser, or the like, to an immunogenic peptide. The lipidated peptide can then be administered either directly in a micelle or particle, incorporated into a liposome, or emulsified in an adjuvant, e.g., incomplete Freund&#39;s adjuvant. In a preferred embodiment, a particularly effective immunogenic composition comprises palmitic acid attached to ε- and α-amino groups of Lys, which is attached via linkage, e.g., Ser-Ser, to the amino terminus of the immunogenic peptide. 
     As another example of lipid priming of CTL responses,  E. coli  lipoproteins, such as tripalmitoyl-S-glycerylcysteinlyseryl-serine (P3CSS) can be used to prime virus specific CTL when covalently attached to an appropriate peptide (see, e.g., Deres, et al., Nature 342:561, 1989). Peptides of the invention can be coupled to P3CSS, for example, and the lipopeptide administered to an individual to prime specifically an immune response to the target antigen. Moreover, because the induction of neutralizing antibodies can also be primed with P3CSS-conjugated epitopes, two such compositions can be combined to more effectively elicit both humoral and cell-mediated responses. 
     X.C.4. Vaccine Compositions Comprising DC Pulsed with CTL and/or HTL Peptides 
     An embodiment of a vaccine composition in accordance with the invention comprises ex vivo administration of a cocktail of epitope-bearing peptides to PBMC, or isolated DC therefrom, from the patient&#39;s blood. A pharmaceutical to facilitate harvesting of DC can be used, such as Progenipoietin™ (Pharmacia-Monsanto, St. Louis, Mo.) or GM-CSF/IL-4. After pulsing the DC with peptides and prior to reinfusion into patients, the DC are washed to remove unbound peptides. In this embodiment, a vaccine comprises peptide-pulsed DCs which present the pulsed peptide epitopes complexed with HLA molecules on their surfaces. 
     The DC can be pulsed ex vivo with a cocktail of peptides, some of which stimulate CTL responses to 158P3D2. Optionally, a helper T cell (HTL) peptide, such as a natural or artificial loosely restricted HLA Class II peptide, can be included to facilitate the CTL response. Thus, a vaccine in accordance with the invention is used to treat a cancer which expresses or overexpresses 158P3D2. 
     X.D.) Adoptive Immunotherapy 
     Antigenic 158P3D2-related peptides are used to elicit a CTL and/or HTL response ex vivo, as well. The resulting CTL or HTL cells, can be used to treat tumors in patients that do not respond to other conventional forms of therapy, or will not respond to a therapeutic vaccine peptide or nucleic acid in accordance with the invention. Ex vivo CTL or HTL responses to a particular antigen are induced by incubating in tissue culture the patient&#39;s, or genetically compatible, CTL or HTL precursor cells together with a source of antigen-presenting cells (APC), such as dendritic cells, and the appropriate immunogenic peptide. After an appropriate incubation time (typically about 7-28 days), in which the precursor cells are activated and expanded into effector cells, the cells are infused back into the patient, where they will destroy (CTL) or facilitate destruction (HTL) of their specific target cell (e.g., a tumor cell). Transfected dendritic cells may also be used as antigen presenting cells. 
     X.E.) Administration of Vaccines for Therapeutic or Prophylactic Purposes 
     Pharmaceutical and vaccine compositions of the invention are typically used to treat and/or prevent a cancer that expresses or overexpresses 158P3D2. In therapeutic applications, peptide and/or nucleic acid compositions are administered to a patient in an amount sufficient to elicit an effective B cell, CTL and/or HTL response to the antigen and to cure or at least partially arrest or slow symptoms and/or complications. An amount adequate to accomplish this is defined as “therapeutically effective dose.” Amounts effective for this use will depend on, e.g., the particular composition administered, the manner of administration, the stage and severity of the disease being treated, the weight and general state of health of the patient, and the judgment of the prescribing physician. 
     For pharmaceutical compositions, the immunogenic peptides of the invention, or DNA encoding them, are generally administered to an individual already bearing a tumor that expresses 158P3D2. The peptides or DNA encoding them can be administered individually or as fusions of one or more peptide sequences. Patients can be treated with the immunogenic peptides separately or in conjunction with other treatments, such as surgery, as appropriate. 
     For therapeutic use, administration should generally begin at the first diagnosis of 158P3D2-associated cancer. This is followed by boosting doses until at least symptoms are substantially abated and for a period thereafter. The embodiment of the vaccine composition (i.e., including, but not limited to embodiments such as peptide cocktails, polyepitopic polypeptides, minigenes, or TAA-specific CTLs or pulsed dendritic cells) delivered to the patient may vary according to the stage of the disease or the patient&#39;s health status. For example, in a patient with a tumor that expresses 158P3D2, a vaccine comprising 158P3D2-specific CTL may be more efficacious in killing tumor cells in patient with advanced disease than alternative embodiments. 
     It is generally important to provide an amount of the peptide epitope delivered by a mode of administration sufficient to stimulate effectively a cytotoxic T cell response; compositions which stimulate helper T cell responses can also be given in accordance with this embodiment of the invention. 
     The dosage for an initial therapeutic immunization generally occurs in a unit dosage range where the lower value is about 1, 5, 50, 500, or 1,000 μg and the higher value is about 10,000; 20,000; 30,000; or 50,000 μg. Dosage values for a human typically range from about 500 μg to about 50,000 μg per 70 kilogram patient. Boosting dosages of between about 1.0 μg to about 50,000 μg of peptide pursuant to a boosting regimen over weeks to months may be administered depending upon the patient&#39;s response and condition as determined by measuring the specific activity of CTL and HTL obtained from the patient&#39;s blood. Administration should continue until at least clinical symptoms or laboratory tests indicate that the neoplasia, has been eliminated or reduced and for a period thereafter. The dosages, routes of administration, and dose schedules are adjusted in accordance with methodologies known in the art. 
     In certain embodiments, the peptides and compositions of the present invention are employed in serious disease states, that is, life-threatening or potentially life threatening situations. In such cases, as a result of the minimal amounts of extraneous substances and the relative nontoxic nature of the peptides in preferred compositions of the invention, it is possible and may be felt desirable by the treating physician to administer substantial excesses of these peptide compositions relative to these stated dosage amounts. 
     The vaccine compositions of the invention can also be used purely as prophylactic agents. Generally the dosage for an initial prophylactic immunization generally occurs in a unit dosage range where the lower value is about 1, 5, 50, 500, or 1000 μg and the higher value is about 10,000; 20,000; 30,000; or 50,000 μg. Dosage values for a human typically range from about 500 μg to about 50,000 μg per 70 kilogram patient. This is followed by boosting dosages of between about 1.0 μg to about 50,000 μg of peptide administered at defined intervals from about four weeks to six months after the initial administration of vaccine. The immunogenicity of the vaccine can be assessed by measuring the specific activity of CTL and HTL obtained from a sample of the patient&#39;s blood. 
     The pharmaceutical compositions for therapeutic treatment are intended for parenteral, topical, oral, nasal, intrathecal, or local (e.g. as a cream or topical ointment) administration. Preferably, the pharmaceutical compositions are administered parentally, e.g., intravenously, subcutaneously, intradermally, or intramuscularly. Thus, the invention provides compositions for parenteral administration which comprise a solution of the immunogenic peptides dissolved or suspended in an acceptable carrier, preferably an aqueous carrier. 
     A variety of aqueous carriers may be used, e.g., water, buffered water, 0.8% saline, 0.3% glycine, hyaluronic acid and the like. These compositions may be sterilized by conventional, well-known sterilization techniques, or may be sterile filtered. The resulting aqueous solutions may be packaged for use as is, or lyophilized, the lyophilized preparation being combined with a sterile solution prior to administration. 
     The compositions may contain pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions, such as pH-adjusting and buffering agents, tonicity adjusting agents, wetting agents, preservatives, and the like, for example, sodium acetate, sodium lactate, sodium chloride, potassium chloride, calcium chloride, sorbitan monolaurate, triethanolamine oleate, etc. 
     The concentration of peptides of the invention in the pharmaceutical formulations can vary widely, i.e., from less than about 0.1%, usually at or at least about 2% to as much as 20% to 50% or more by weight, and will be selected primarily by fluid volumes, viscosities, etc., in accordance with the particular mode of administration selected. 
     A human unit dose form of a composition is typically included in a pharmaceutical composition that comprises a human unit dose of an acceptable carrier, in one embodiment an aqueous carrier, and is administered in a volume/quantity that is known by those of skill in the art to be used for administration of such compositions to humans (see, e.g., Remington&#39;s Pharmaceutical Sciences, 17th Edition, A. Gennaro, Editor, Mack Publishing Co., Easton, Pa., 1985). For example a peptide dose for initial immunization can be from about 1 to about 50,000 μg, generally 100-5,000 μg, for a 70 kg patient. For example, for nucleic acids an initial immunization may be performed using an expression vector in the form of naked nucleic acid administered IM (or SC or ID) in the amounts of 0.5-5 mg at multiple sites. The nucleic acid (0.1 to 1000 μg) can also be administered using a gene gun. Following an incubation period of 3-4 weeks, a booster dose is then administered. The booster can be recombinant fowlpox virus administered at a dose of 5-107 to 5×109 pfu. 
     For antibodies, a treatment generally involves repeated administration of the anti-158P3D2 antibody preparation, via an acceptable route of administration such as intravenous injection (IV), typically at a dose in the range of about 0.1 to about 10 mg/kg body weight. In general, doses in the range of 10-500 mg mAb per week are effective and well tolerated. Moreover, an initial loading dose of approximately 4 mg/kg patient body weight IV, followed by weekly doses of about 2 mg/kg IV of the anti-158P3D2 mAb preparation represents an acceptable dosing regimen. As appreciated by those of skill in the art, various factors can influence the ideal dose in a particular case. Such factors include, for example, half life of a composition, the binding affinity of an Ab, the immunogenicity of a substance, the degree of 158P3D2 expression in the patient, the extent of circulating shed 158P3D2 antigen, the desired steady-state concentration level, frequency of treatment, and the influence of chemotherapeutic or other agents used in combination with the treatment method of the invention, as well as the health status of a particular patient. Non-limiting preferred human unit doses are, for example, 500 μg-1 mg, 1 mg-50 mg, 50 mg-100 mg, 100 mg-200 mg, 200 mg-300 mg, 400 mg-500 mg, 500 mg-600 mg, 600 mg-700 mg, 700 mg-800 mg, 800 mg-900 mg, 900 mg-1 g, or 1 mg-700 mg. In certain embodiments, the dose is in a range of 2-5 mg/kg body weight, e.g., with follow on weekly doses of 1-3 mg/kg; 0.5 mg, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 mg/kg body weight followed, e.g., in two, three or four weeks by weekly doses; 0.5-10 mg/kg body weight, e.g., followed in two, three or four weeks by weekly doses; 225, 250, 275, 300, 325, 350, 375, 400 mg m2 of body area weekly; 1-600 mg m2 of body area weekly; 225-400 mg m2 of body area weekly; these does can be followed by weekly doses for 2, 3, 4, 5, 6, 7, 8, 9, 19, 11, 12 or more weeks. 
     In one embodiment, human unit dose forms of polynucleotides comprise a suitable dosage range or effective amount that provides any therapeutic effect. As appreciated by one of ordinary skill in the art a therapeutic effect depends on a number of factors, including the sequence of the polynucleotide, molecular weight of the polynucleotide and route of administration. Dosages are generally selected by the physician or other health care professional in accordance with a variety of parameters known in the art, such as severity of symptoms, history of the patient and the like. Generally, for a polynucleotide of about 20 bases, a dosage range may be selected from, for example, an independently selected lower limit such as about 0.1, 0.25, 0.5, 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400 or 500 mg/kg up to an independently selected upper limit, greater than the lower limit, of about 60, 80, 100, 200, 300, 400, 500, 750, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000 or 10,000 mg/kg. For example, a dose may be about any of the following: 0.1 to 100 mg/kg, 0.1 to 50 mg/kg, 0.1 to 25 mg/kg, 0.1 to 10 mg/kg, 1 to 500 mg/kg, 100 to 400 mg/kg, 200 to 300 mg/kg, 1 to 100 mg/kg, 100 to 200 mg/kg, 300 to 400 mg/kg, 400 to 500 mg/kg, 500 to 1000 mg/kg, 500 to 5000 mg/kg, or 500 to 10,000 mg/kg. Generally, parenteral routes of administration may require higher doses of polynucleotide compared to more direct application to the nucleotide to diseased tissue, as do polynucleotides of increasing length. 
     In one embodiment, human unit dose forms of T-cells comprise a suitable dosage range or effective amount that provides any therapeutic effect. As appreciated by one of ordinary skill in the art, a therapeutic effect depends on a number of factors. Dosages are generally selected by the physician or other health care professional in accordance with a variety of parameters known in the art, such as severity of symptoms, history of the patient and the like. A dose may be about 104 cells to about 106 cells, about 106 cells to about 108 cells, about 108 to about 1011 cells, or about 108 to about 5×1010 cells. A dose may also about 106 cells/m2 to about 1010 cells/m2, or about 106 cells/m2 to about 108 cells/m2. 
     Proteins(s) of the invention, and/or nucleic acids encoding the protein(s), can also be administered via liposomes, which may also serve to: 1) target the proteins(s) to a particular tissue, such as lymphoid tissue; 2) to target selectively to diseases cells; or, 3) to increase the half-life of the peptide composition. Liposomes include emulsions, foams, micelles, insoluble monolayers, liquid crystals, phospholipid dispersions, lamellar layers and the like. In these preparations, the peptide to be delivered is incorporated as part of a liposome, alone or in conjunction with a molecule which binds to a receptor prevalent among lymphoid cells, such as monoclonal antibodies which bind to the CD45 antigen, or with other therapeutic or immunogenic compositions. Thus, liposomes either filled or decorated with a desired peptide of the invention can be directed to the site of lymphoid cells, where the liposomes then deliver the peptide compositions. Liposomes for use in accordance with the invention are formed from standard vesicle-forming lipids, which generally include neutral and negatively charged phospholipids and a sterol, such as cholesterol. The selection of lipids is generally guided by consideration of, e.g., liposome size, acid lability and stability of the liposomes in the blood stream. A variety of methods are available for preparing liposomes, as described in, e.g., Szoka, et al., Ann Rev. Biophys. Bioeng. 9:467 (1980), and U.S. Pat. Nos. 4,235,871, 4,501,728, 4,837,028, and 5,019,369. 
     For targeting cells of the immune system, a ligand to be incorporated into the liposome can include, e.g., antibodies or fragments thereof specific for cell surface determinants of the desired immune system cells. A liposome suspension containing a peptide may be administered intravenously, locally, topically, etc. in a dose which varies according to, inter alia, the manner of administration, the peptide being delivered, and the stage of the disease being treated. 
     For solid compositions, conventional nontoxic solid carriers may be used which include, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharin, talcum, cellulose, glucose, sucrose, magnesium carbonate, and the like. For oral administration, a pharmaceutically acceptable nontoxic composition is formed by incorporating any of the normally employed excipients, such as those carriers previously listed, and generally 10-95% of active ingredient, that is, one or more peptides of the invention, and more preferably at a concentration of 25%-75%. 
     For aerosol administration, immunogenic peptides are preferably supplied in finely divided form along with a surfactant and propellant. Typical percentages of peptides are about 0.01%-20% by weight, preferably about 1%-10%. The surfactant must, of course, be nontoxic, and preferably soluble in the propellant. Representative of such agents are the esters or partial esters of fatty acids containing from about 6 to 22 carbon atoms, such as caproic, octanoic, lauric, palmitic, stearic, linoleic, linolenic, olesteric and oleic acids with an aliphatic polyhydric alcohol or its cyclic anhydride. Mixed esters, such as mixed or natural glycerides may be employed. The surfactant may constitute about 0.1%-20% by weight of the composition, preferably about 0.25-5%. The balance of the composition is ordinarily propellant. A carrier can also be included, as desired, as with, e.g., lecithin for intranasal delivery. 
     XI.) Diagnostic and Prognostic Embodiments of 158P3D2 
     As disclosed herein, 158P3D2 polynucleotides, polypeptides, reactive cytotoxic T cells (CTL), reactive helper T cells (HTL) and anti-polypeptide antibodies are used in well known diagnostic, prognostic and therapeutic assays that examine conditions associated with dysregulated cell growth such as cancer, in particular the cancers listed in Table I (see, e.g., both its specific pattern of tissue expression as well as its overexpression in certain cancers as described for example in the Example entitled “Expression analysis of 158P3D2 in normal tissues, and patient specimens”). 
     158P3D2 can be analogized to a prostate associated antigen PSA, the archetypal marker that has been used by medical practitioners for years to identify and monitor the presence of prostate cancer (see, e.g., Merrill et al., J. Urol. 163(2): 503-5120 (2000); Polascik et al., J. Urol. August; 162(2):293-306 (1999) and Fortier et al., J. Nat. Cancer Inst. 91(19): 1635-1640 (1999)). A variety of other diagnostic markers are also used in similar contexts including p53 and K-ras (see, e.g., Tulchinsky et al., Int J Mol Med 1999 Jul. 4(1):99-102 and Minimoto et al., Cancer Detect Prey 2000; 24(1):1-12). Therefore, this disclosure of 158P3D2 polynucleotides and polypeptides (as well as 158P3D2 polynucleotide probes and anti-158P3D2 antibodies used to identify the presence of these molecules) and their properties allows skilled artisans to utilize these molecules in methods that are analogous to those used, for example, in a variety of diagnostic assays directed to examining conditions associated with cancer. 
     Typical embodiments of diagnostic methods which utilize the 158P3D2 polynucleotides, polypeptides, reactive T cells and antibodies are analogous to those methods from well-established diagnostic assays, which employ, e.g., PSA polynucleotides, polypeptides, reactive T cells and antibodies. For example, just as PSA polynucleotides are used as probes (for example in Northern analysis, see, e.g., Sharief et al., Biochem. Mol. Biol. Int. 33(3):567-74 (1994)) and primers (for example in PCR analysis, see, e.g., Okegawa et al., J. Urol. 163(4): 1189-1190 (2000)) to observe the presence and/or the level of PSA mRNAs in methods of monitoring PSA overexpression or the metastasis of prostate cancers, the 158P3D2 polynucleotides described herein can be utilized in the same way to detect 158P3D2 overexpression or the metastasis of prostate and other cancers expressing this gene. Alternatively, just as PSA polypeptides are used to generate antibodies specific for PSA which can then be used to observe the presence and/or the level of PSA proteins in methods to monitor PSA protein overexpression (see, e.g., Stephan et al., Urology 55(4):560-3 (2000)) or the metastasis of prostate cells (see, e.g., Alanen et al., Pathol. Res. Pract. 192(3):233-7 (1996)), the 158P3D2 polypeptides described herein can be utilized to generate antibodies for use in detecting 158P3D2 overexpression or the metastasis of prostate cells and cells of other cancers expressing this gene. 
     Specifically, because metastases involves the movement of cancer cells from an organ of origin (such as the lung or prostate gland etc.) to a different area of the body (such as a lymph node), assays which examine a biological sample for the presence of cells expressing 158P3D2 polynucleotides and/or polypeptides can be used to provide evidence of metastasis. For example, when a biological sample from tissue that does not normally contain 158P3D2-expressing cells (lymph node) is found to contain 158P3D2-expressing cells such as the 158P3D2 expression seen in LAPC4 and LAPC9, xenografts isolated from lymph node and bone metastasis, respectively, this finding is indicative of metastasis. 
     Alternatively 158P3D2 polynucleotides and/or polypeptides can be used to provide evidence of cancer, for example, when cells in a biological sample that do not normally express 158P3D2 or express 158P3D2 at a different level are found to express 158P3D2 or have an increased expression of 158P3D2 (see, e.g., the 158P3D2 expression in the cancers listed in Table I and in patient samples etc. shown in the accompanying Figures). In such assays, artisans may further wish to generate supplementary evidence of metastasis by testing the biological sample for the presence of a second tissue restricted marker (in addition to 158P3D2) such as PSA, PSCA etc. (see, e.g., Alanen et al., Pathol. Res. Pract. 192(3): 233-237 (1996)). 
     The use of immunohistochemistry to identify the presence of a 158P3D2 polypeptide within a tissue section can indicate an altered state of certain cells within that tissue. It is well understood in the art that the ability of an antibody to localize to a polypeptide that is expressed in cancer cells is a way of diagnosing presence of disease, disease stage, progression and/or tumor aggressiveness. Such an antibody can also detect an altered distribution of the polypeptide within the cancer cells, as compared to corresponding non-malignant tissue. 
     The 158P3D2 polypeptide and immunogenic compositions are also useful in view of the phenomena of altered subcellular protein localization in disease states. Alteration of cells from normal to diseased state causes changes in cellular morphology and is often associated with changes in subcellular protein localization/distribution. For example, cell membrane proteins that are expressed in a polarized manner in normal cells can be altered in disease, resulting in distribution of the protein in a non-polar manner over the whole cell surface. 
     The phenomenon of altered subcellular protein localization in a disease state has been demonstrated with MUC1 and Her2 protein expression by use of immunohistochemical means. Normal epithelial cells have a typical apical distribution of MUC1, in addition to some supranuclear localization of the glycoprotein, whereas malignant lesions often demonstrate an apolar staining pattern (Diaz et al, The Breast Journal, 7; 40-45 (2001); Zhang et al, Clinical Cancer Research, 4; 2669-2676 (1998): Cao, et al, The Journal of Histochemistry and Cytochemistry, 45: 1547-1557 (1997)). In addition, normal breast epithelium is either negative for Her2 protein or exhibits only a basolateral distribution whereas malignant cells can express the protein over the whole cell surface (De Potter, et al, International Journal of Cancer, 44; 969-974 (1989): McCormick, et al, 117; 935-943 (2002)). Alternatively, distribution of the protein may be altered from a surface only localization to include diffuse cytoplasmic expression in the diseased state. Such an example can be seen with MUC1 (Diaz, et al, The Breast Journal, 7: 40-45 (2001)). 
     Alteration in the localization/distribution of a protein in the cell, as detected by immunohistochemical methods, can also provide valuable information concerning the favorability of certain treatment modalities. This last point is illustrated by a situation where a protein may be intracellular in normal tissue, but cell surface in malignant cells; the cell surface location makes the cells favorably amenable to antibody-based diagnostic and treatment regimens. When such an alteration of protein localization occurs for 158P3D2, the 158P3D2 protein and immune responses related thereto are very useful. Accordingly, the ability to determine whether alteration of subcellular protein localization occurred for 24P4C12 make the 158P3D2 protein and immune responses related thereto very useful. Use of the 158P3D2 compositions allows those skilled in the art to make important diagnostic and therapeutic decisions. 
     Immunohistochemical reagents specific to 158P3D2 are also useful to detect metastases of tumors expressing 158P3D2 when the polypeptide appears in tissues where 158P3D2 is not normally produced. 
     Thus, 158P3D2 polypeptides and antibodies resulting from immune responses thereto are useful in a variety of important contexts such as diagnostic, prognostic, preventative and/or therapeutic purposes known to those skilled in the art. 
     Just as PSA polynucleotide fragments and polynucleotide variants are employed by skilled artisans for use in methods of monitoring PSA, 158P3D2 polynucleotide fragments and polynucleotide variants are used in an analogous manner. In particular, typical PSA polynucleotides used in methods of monitoring PSA are probes or primers which consist of fragments of the PSA cDNA sequence. Illustrating this, primers used to PCR amplify a PSA polynucleotide must include less than the whole PSA sequence to function in the polymerase chain reaction. In the context of such PCR reactions, skilled artisans generally create a variety of different polynucleotide fragments that can be used as primers in order to amplify different portions of a polynucleotide of interest or to optimize amplification reactions (see, e.g., Caetano-Anolles, G. Biotechniques 25(3): 472-476, 478-480 (1998); Robertson et al., Methods Mol. Biol. 98:121-154 (1998)). An additional illustration of the use of such fragments is provided in the Example entitled “Expression analysis of 158P3D2 in normal tissues, and patient specimens,” where a 158P3D2 polynucleotide fragment is used as a probe to show the expression of 158P3D2 RNAs in cancer cells. In addition, variant polynucleotide sequences are typically used as primers and probes for the corresponding mRNAs in PCR and Northern analyses (see, e.g., Sawai et al., Fetal Diagn. Ther. 1996 November-December 11(6):407-13 and Current Protocols In Molecular Biology, Volume 2, Unit 2, Frederick M. Ausubel et al. eds., 1995)). Polynucleotide fragments and variants are useful in this context where they are capable of binding to a target polynucleotide sequence (e.g., a 158P3D2 polynucleotide shown in  FIG. 2  or variant thereof) under conditions of high stringency. 
     Furthermore, PSA polypeptides which contain an epitope that can be recognized by an antibody or T cell that specifically binds to that epitope are used in methods of monitoring PSA. 158P3D2 polypeptide fragments and polypeptide analogs or variants can also be used in an analogous manner. This practice of using polypeptide fragments or polypeptide variants to generate antibodies (such as anti-PSA antibodies or T cells) is typical in the art with a wide variety of systems such as fusion proteins being used by practitioners (see, e.g., Current Protocols In Molecular Biology, Volume 2, Unit 16, Frederick M. Ausubel et al. eds., 1995). In this context, each epitope(s) functions to provide the architecture with which an antibody or T cell is reactive. Typically, skilled artisans create a variety of different polypeptide fragments that can be used in order to generate immune responses specific for different portions of a polypeptide of interest (see, e.g., U.S. Pat. No. 5,840,501 and U.S. Pat. No. 5,939,533). For example it may be preferable to utilize a polypeptide comprising one of the 158P3D2 biological motifs discussed herein or a motif-bearing subsequence which is readily identified by one of skill in the art based on motifs available in the art. Polypeptide fragments, variants or analogs are typically useful in this context as long as they comprise an epitope capable of generating an antibody or T cell specific for a target polypeptide sequence (e.g. a 158P3D2 polypeptide shown in  FIG. 3 ). 
     As shown herein, the 158P3D2 polynucleotides and polypeptides (as well as the 158P3D2 polynucleotide probes and anti-158P3D2 antibodies or T cells used to identify the presence of these molecules) exhibit specific properties that make them useful in diagnosing cancers such as those listed in Table I. Diagnostic assays that measure the presence of 158P3D2 gene products, in order to evaluate the presence or onset of a disease condition described herein, such as prostate cancer, are used to identify patients for preventive measures or further monitoring, as has been done so successfully with PSA. Moreover, these materials satisfy a need in the art for molecules having similar or complementary characteristics to PSA in situations where, for example, a definite diagnosis of metastasis of prostatic origin cannot be made on the basis of a test for PSA alone (see, e.g., Alanen et al., Pathol. Res. Pract. 192(3): 233-237 (1996)), and consequently, materials such as 158P3D2 polynucleotides and polypeptides (as well as the 158P3D2 polynucleotide probes and anti-158P3D2 antibodies used to identify the presence of these molecules) need to be employed to confirm a metastases of prostatic origin. 
     Finally, in addition to their use in diagnostic assays, the 158P3D2 polynucleotides disclosed herein have a number of other utilities such as their use in the identification of oncogenetic associated chromosomal abnormalities in the chromosomal region to which the 158P3D2 gene maps (see the Example entitled “Chromosomal Mapping of 158P3D2” below). Moreover, in addition to their use in diagnostic assays, the 158P3D2-related proteins and polynucleotides disclosed herein have other utilities such as their use in the forensic analysis of tissues of unknown origin (see, e.g., Takahama K Forensic Sci Int 1996 Jun. 28; 80(1-2): 63-9). 
     Additionally, 158P3D2-related proteins or polynucleotides of the invention can be used to treat a pathologic condition characterized by the over-expression of 158P3D2. For example, the amino acid or nucleic acid sequence of  FIG. 2  or  FIG. 3 , or fragments of either, can be used to generate an immune response to a 158P3D2 antigen. Antibodies or other molecules that react with 158P3D2 can be used to modulate the function of this molecule, and thereby provide a therapeutic benefit. 
     XII.) Inhibition of 158P3D2 Protein Function 
     The invention includes various methods and compositions for inhibiting the binding of 158P3D2 to its binding partner or its association with other protein(s) as well as methods for inhibiting 158P3D2 function. 
     XII.A.) Inhibition of 158P3D2 With Intracellular Antibodies 
     In one approach, a recombinant vector that encodes single chain antibodies that specifically bind to 158P3D2 are introduced into 158P3D2 expressing cells via gene transfer technologies. Accordingly, the encoded single chain anti-158P3D2 antibody is expressed intracellularly, binds to 158P3D2 protein, and thereby inhibits its function. Methods for engineering such intracellular single chain antibodies are well known. Such intracellular antibodies, also known as “intrabodies”, are specifically targeted to a particular compartment within the cell, providing control over where the inhibitory activity of the treatment is focused. This technology has been successfully applied in the art (for review, see Richardson and Marasco, 1995, TIBTECH vol. 13). Intrabodies have been shown to virtually eliminate the expression of otherwise abundant cell surface receptors (see, e.g., Richardson et al., 1995, Proc. Natl. Acad. Sci. USA 92: 3137-3141; Beerli et al., 1994, J. Biol. Chem. 289: 23931-23936; Deshane et al., 1994, Gene Ther. 1: 332-337). 
     Single chain antibodies comprise the variable domains of the heavy and light chain joined by a flexible linker polypeptide, and are expressed as a single polypeptide. Optionally, single chain antibodies are expressed as a single chain variable region fragment joined to the light chain constant region. Well-known intracellular trafficking signals are engineered into recombinant polynucleotide vectors encoding such single chain antibodies in order to target precisely the intrabody to the desired intracellular compartment. For example, intrabodies targeted to the endoplasmic reticulum (ER) are engineered to incorporate a leader peptide and, optionally, a C-terminal ER retention signal, such as the KDEL amino acid motif. Intrabodies intended to exert activity in the nucleus are engineered to include a nuclear localization signal. Lipid moieties are joined to intrabodies in order to tether the intrabody to the cytosolic side of the plasma membrane. Intrabodies can also be targeted to exert function in the cytosol. For example, cytosolic intrabodies are used to sequester factors within the cytosol, thereby preventing them from being transported to their natural cellular destination. 
     In one embodiment, intrabodies are used to capture 158P3D2 in the nucleus, thereby preventing its activity within the nucleus. Nuclear targeting signals are engineered into such 158P3D2 intrabodies in order to achieve the desired targeting. Such 158P3D2 intrabodies are designed to bind specifically to a particular 158P3D2 domain. In another embodiment, cytosolic intrabodies that specifically bind to a 158P3D2 protein are used to prevent 158P3D2 from gaining access to the nucleus, thereby preventing it from exerting any biological activity within the nucleus (e.g., preventing 158P3D2 from forming transcription complexes with other factors). 
     In order to specifically direct the expression of such intrabodies to particular cells, the transcription of the intrabody is placed under the regulatory control of an appropriate tumor-specific promoter and/or enhancer. In order to target intrabody expression specifically to prostate, for example, the PSA promoter and/or promoter/enhancer can be utilized (See, for example, U.S. Pat. No. 5,919,652 issued 6 Jul. 1999). 
     XII.B.) Inhibition of 158P3D2 with Recombinant Proteins 
     In another approach, recombinant molecules bind to 158P3D2 and thereby inhibit 158P3D2 function. For example, these recombinant molecules prevent or inhibit 158P3D2 from accessing/binding to its binding partner(s) or associating with other protein(s). Such recombinant molecules can, for example, contain the reactive part(s) of a 158P3D2 specific antibody molecule. In a particular embodiment, the 158P3D2 binding domain of a 158P3D2 binding partner is engineered into a dimeric fusion protein, whereby the fusion protein comprises two 158P3D2 ligand binding domains linked to the Fc portion of a human IgG, such as human IgG1. Such IgG portion can contain, for example, the CH2 and CH3 domains and the hinge region, but not the CH1 domain. Such dimeric fusion proteins are administered in soluble form to patients suffering from a cancer associated with the expression of 158P3D2, whereby the dimeric fusion protein specifically binds to 158P3D2 and blocks 158P3D2 interaction with a binding partner. Such dimeric fusion proteins are further combined into multimeric proteins using known antibody linking technologies. 
     XII.C.) Inhibition of 158P3D2 Transcription or Translation 
     The present invention also comprises various methods and compositions for inhibiting the transcription of the 158P3D2 gene. Similarly, the invention also provides methods and compositions for inhibiting the translation of 158P3D2 mRNA into protein. 
     In one approach, a method of inhibiting the transcription of the 158P3D2 gene comprises contacting the 158P3D2 gene with a 158P3D2 antisense polynucleotide. In another approach, a method of inhibiting 158P3D2 mRNA translation comprises contacting a 158P3D2 mRNA with an antisense polynucleotide. In another approach, a 158P3D2 specific ribozyme is used to cleave a 158P3D2 message, thereby inhibiting translation. Such antisense and ribozyme based methods can also be directed to the regulatory regions of the 158P3D2 gene, such as 158P3D2 promoter and/or enhancer elements. Similarly, proteins capable of inhibiting a 158P3D2 gene transcription factor are used to inhibit 158P3D2 mRNA transcription. The various polynucleotides and compositions useful in the aforementioned methods have been described above. The use of antisense and ribozyme molecules to inhibit transcription and translation is well known in the art. 
     Other factors that inhibit the transcription of 158P3D2 by interfering with 158P3D2 transcriptional activation are also useful to treat cancers expressing 158P3D2. Similarly, factors that interfere with 158P3D2 processing are useful to treat cancers that express 158P3D2. Cancer treatment methods utilizing such factors are also within the scope of the invention. 
     XII.D.) General Considerations for Therapeutic Strategies 
     Gene transfer and gene therapy technologies can be used to deliver therapeutic polynucleotide molecules to tumor cells synthesizing 158P3D2 (i.e., antisense, ribozyme, polynucleotides encoding intrabodies and other 158P3D2 inhibitory molecules). A number of gene therapy approaches are known in the art. Recombinant vectors encoding 158P3D2 antisense polynucleotides, ribozymes, factors capable of interfering with 158P3D2 transcription, and so forth, can be delivered to target tumor cells using such gene therapy approaches. 
     The above therapeutic approaches can be combined with any one of a wide variety of surgical, chemotherapy or radiation therapy regimens. The therapeutic approaches of the invention can enable the use of reduced dosages of chemotherapy (or other therapies) and/or less frequent administration, an advantage for all patients and particularly for those that do not tolerate the toxicity of the chemotherapeutic agent well. 
     The anti-tumor activity of a particular composition (e.g., antisense, ribozyme, intrabody), or a combination of such compositions, can be evaluated using various in vitro and in vivo assay systems. In vitro assays that evaluate therapeutic activity include cell growth assays, soft agar assays and other assays indicative of tumor promoting activity, binding assays capable of determining the extent to which a therapeutic composition will inhibit the binding of 158P3D2 to a binding partner, etc. 
     In vivo, the effect of a 158P3D2 therapeutic composition can be evaluated in a suitable animal model. For example, xenogenic prostate cancer models can be used, wherein human prostate cancer explants or passaged xenograft tissues are introduced into immune compromised animals, such as nude or SCID mice (Klein et al., 1997, Nature Medicine 3: 402-408). For example, PCT Patent Application WO98/16628 and U.S. Pat. No. 6,107,540 describe various xenograft models of human prostate cancer capable of recapitulating the development of primary tumors, micrometastasis, and the formation of osteoblastic metastases characteristic of late stage disease. Efficacy can be predicted using assays that measure inhibition of tumor formation, tumor regression or metastasis, and the like. 
     In vivo assays that evaluate the promotion of apoptosis are useful in evaluating therapeutic compositions. In one embodiment, xenografts from tumor bearing mice treated with the therapeutic composition can be examined for the presence of apoptotic foci and compared to untreated control xenograft-bearing mice. The extent to which apoptotic foci are found in the tumors of the treated mice provides an indication of the therapeutic efficacy of the composition. 
     The therapeutic compositions used in the practice of the foregoing methods can be formulated into pharmaceutical compositions comprising a carrier suitable for the desired delivery method. Suitable carriers include any material that when combined with the therapeutic composition retains the anti-tumor function of the therapeutic composition and is generally non-reactive with the patient&#39;s immune system. Examples include, but are not limited to, any of a number of standard pharmaceutical carriers such as sterile phosphate buffered saline solutions, bacteriostatic water, and the like (see, generally, Remington&#39;s Pharmaceutical Sciences 16th Edition, A. Osal., Ed., 1980). 
     Therapeutic formulations can be solubilized and administered via any route capable of delivering the therapeutic composition to the tumor site. Potentially effective routes of administration include, but are not limited to, intravenous, parenteral, intraperitoneal, intramuscular, intratumor, intradermal, intraorgan, orthotopic, and the like. A preferred formulation for intravenous injection comprises the therapeutic composition in a solution of preserved bacteriostatic water, sterile unpreserved water, and/or diluted in polyvinylchloride or polyethylene bags containing 0.9% sterile Sodium Chloride for Injection, USP. Therapeutic protein preparations can be lyophilized and stored as sterile powders, preferably under vacuum, and then reconstituted in bacteriostatic water (containing for example, benzyl alcohol preservative) or in sterile water prior to injection. 
     Dosages and administration protocols for the treatment of cancers using the foregoing methods will vary with the method and the target cancer, and will generally depend on a number of other factors appreciated in the art. 
     XIII.) Identification, Characterization and Use of Modulators of 158P3D2 
     XIII.A.) Methods to Identify and Use Modulators 
     In one embodiment, screening is performed to identify modulators that induce or suppress a particular expression profile, suppress or induce specific pathways, preferably generating the associated phenotype thereby. In another embodiment, having identified differentially expressed genes important in a particular state; screens are performed to identify modulators that alter expression of individual genes, either increase or decrease. In another embodiment, screening is performed to identify modulators that alter a biological function of the expression product of a differentially expressed gene. Again, having identified the importance of a gene in a particular state, screens are performed to identify agents that bind and/or modulate the biological activity of the gene product. 
     In addition, screens are done for genes that are induced in response to a candidate agent. After identifying a modulator (one that suppresses a cancer expression pattern leading to a normal expression pattern, or a modulator of a cancer gene that leads to expression of the gene as in normal tissue) a screen is performed to identify genes that are specifically modulated in response to the agent. Comparing expression profiles between normal tissue and agent-treated cancer tissue reveals genes that are not expressed in normal tissue or cancer tissue, but are expressed in agent treated tissue, and vice versa. These agent-specific sequences are identified and used by methods described herein for cancer genes or proteins. In particular these sequences and the proteins they encode are used in marking or identifying agent-treated cells. In addition, antibodies are raised against the agent-induced proteins and used to target novel therapeutics to the treated cancer tissue sample. 
     XIII.B.) Gene Expression-related Assays 
     Proteins, nucleic acids, and antibodies of the invention are used in screening assays. The cancer-associated proteins, antibodies, nucleic acids, modified proteins and cells containing these sequences are used in screening assays, such as evaluating the effect of drug candidates on a “gene expression profile,” expression profile of polypeptides or alteration of biological function. In one embodiment, the expression profiles are used, preferably in conjunction with high throughput screening techniques to allow monitoring for expression profile genes after treatment with a candidate agent (e.g., Davis, G F, et al, J Biol Screen 7:69 (2002); Zlokamik, et al., Science 279:84-8 (1998); Heid, Genome Res 6:986-94, 1996). 
     The cancer proteins, antibodies, nucleic acids, modified proteins and cells containing the native or modified cancer proteins or genes are used in screening assays. That is, the present invention comprises methods for screening for compositions which modulate the cancer phenotype or a physiological function of a cancer protein of the invention. This is done on a gene itself or by evaluating the effect of drug candidates on a “gene expression profile” or biological function. In one embodiment, expression profiles are used, preferably in conjunction with high throughput screening techniques to allow monitoring after treatment with a candidate agent, see Zlokarnik, supra. 
     A variety of assays are executed directed to the genes and proteins of the invention. Assays are run on an individual nucleic acid or protein level. That is, having identified a particular gene as up regulated in cancer, test compounds are screened for the ability to modulate gene expression or for binding to the cancer protein of the invention. “Modulation” in this context includes an increase or a decrease in gene expression. The preferred amount of modulation will depend on the original change of the gene expression in normal versus tissue undergoing cancer, with changes of at least 10%, preferably 50%, more preferably 100-300%, and in some embodiments 300-1000% or greater. Thus, if a gene exhibits a 4-fold increase in cancer tissue compared to normal tissue, a decrease of about four-fold is often desired; similarly, a 10-fold decrease in cancer tissue compared to normal tissue a target value of a 10-fold increase in expression by the test compound is often desired. Modulators that exacerbate the type of gene expression seen in cancer are also useful, e.g., as an upregulated target in further analyses. 
     The amount of gene expression is monitored using nucleic acid probes and the quantification of gene expression levels, or, alternatively, a gene product itself is monitored, e.g., through the use of antibodies to the cancer protein and standard immunoassays. Proteomics and separation techniques also allow for quantification of expression. 
     XIII.C.) Expression Monitoring to Identify Compounds that Modify Gene Expression 
     In one embodiment, gene expression monitoring, i.e., an expression profile, is monitored simultaneously for a number of entities. Such profiles will typically involve one or more of the genes of  FIG. 2 . In this embodiment, e.g., cancer nucleic acid probes are attached to biochips to detect and quantify cancer sequences in a particular cell. Alternatively, PCR can be used. Thus, a series, e.g., wells of a microtiter plate, can be used with dispensed primers in desired wells. A PCR reaction can then be performed and analyzed for each well. 
     Expression monitoring is performed to identify compounds that modify the expression of one or more cancer-associated sequences, e.g., a polynucleotide sequence set out in  FIG. 2 . Generally, a test modulator is added to the cells prior to analysis. Moreover, screens are also provided to identify agents that modulate cancer, modulate cancer proteins of the invention, bind to a cancer protein of the invention, or interfere with the binding of a cancer protein of the invention and an antibody or other binding partner. 
     In one embodiment, high throughput screening methods involve providing a library containing a large number of potential therapeutic compounds (candidate compounds). Such “combinatorial chemical libraries” are then screened in one or more assays to identify those library members (particular chemical species or subclasses) that display a desired characteristic activity. The compounds thus identified can serve as conventional “lead compounds,” as compounds for screening, or as therapeutics. 
     In certain embodiments, combinatorial libraries of potential modulators are screened for an ability to bind to a cancer polypeptide or to modulate activity. Conventionally, new chemical entities with useful properties are generated by identifying a chemical compound (called a “lead compound”) with some desirable property or activity, e.g., inhibiting activity, creating variants of the lead compound, and evaluating the property and activity of those variant compounds. Often, high throughput screening (HTS) methods are employed for such an analysis. 
     As noted above, gene expression monitoring is conveniently used to test candidate modulators (e.g., protein, nucleic acid or small molecule). After the candidate agent has been added and the cells allowed to incubate for a period, the sample containing a target sequence to be analyzed is, e.g., added to a biochip. 
     If required, the target sequence is prepared using known techniques. For example, a sample is treated to lyse the cells, using known lysis buffers, electroporation, etc., with purification and/or amplification such as PCR performed as appropriate. For example, an in vitro transcription with labels covalently attached to the nucleotides is performed. Generally, the nucleic acids are labeled with biotin-FITC or PE, or with cy3 or cy5. 
     The target sequence can be labeled with, e.g., a fluorescent, a chemiluminescent, a chemical, or a radioactive signal, to provide a means of detecting the target sequence&#39;s specific binding to a probe. The label also can be an enzyme, such as alkaline phosphatase or horseradish peroxidase, which when provided with an appropriate substrate produces a product that is detected. Alternatively, the label is a labeled compound or small molecule, such as an enzyme inhibitor, that binds but is not catalyzed or altered by the enzyme. The label also can be a moiety or compound, such as, an epitope tag or biotin which specifically binds to streptavidin. For the example of biotin, the streptavidin is labeled as described above, thereby, providing a detectable signal for the bound target sequence. Unbound labeled streptavidin is typically removed prior to analysis. 
     As will be appreciated by those in the art, these assays can be direct hybridization assays or can comprise “sandwich assays”, which include the use of multiple probes, as is generally outlined in U.S. Pat. Nos. 5,681,702; 5,597,909; 5,545,730; 5,594,117; 5,591,584; 5,571,670; 5,580,731; 5,571,670; 5,591,584; 5,624,802; 5,635,352; 5,594,118; 5,359,100; 5,124,246; and 5,681,697. In this embodiment, in general, the target nucleic acid is prepared as outlined above, and then added to the biochip comprising a plurality of nucleic acid probes, under conditions that allow the formation of a hybridization complex. 
     A variety of hybridization conditions are used in the present invention, including high, moderate and low stringency conditions as outlined above. The assays are generally run under stringency conditions which allow formation of the label probe hybridization complex only in the presence of target. Stringency can be controlled by altering a step parameter that is a thermodynamic variable, including, but not limited to, temperature, formamide concentration, salt concentration, chaotropic salt concentration pH, organic solvent concentration, etc. These parameters may also be used to control non-specific binding, as is generally outlined in U.S. Pat. No. 5,681,697. Thus, it can be desirable to perform certain steps at higher stringency conditions to reduce non-specific binding. 
     The reactions outlined herein can be accomplished in a variety of ways. Components of the reaction can be added simultaneously, or sequentially, in different orders, with preferred embodiments outlined below. In addition, the reaction may include a variety of other reagents. These include salts, buffers, neutral proteins, e.g. albumin, detergents, etc. which can be used to facilitate optimal hybridization and detection, and/or reduce nonspecific or background interactions. Reagents that otherwise improve the efficiency of the assay, such as protease inhibitors, nuclease inhibitors, anti-microbial agents, etc., may also be used as appropriate, depending on the sample preparation methods and purity of the target. The assay data are analyzed to determine the expression levels of individual genes, and changes in expression levels as between states, forming a gene expression profile. 
     XIII.D.) Biological Activity-Related Assays 
     The invention provides methods identify or screen for a compound that modulates the activity of a cancer-related gene or protein of the invention. The methods comprise adding a test compound, as defined above, to a cell comprising a cancer protein of the invention. The cells contain a recombinant nucleic acid that encodes a cancer protein of the invention. In another embodiment, a library of candidate agents is tested on a plurality of cells. 
     In one aspect, the assays are evaluated in the presence or absence or previous or subsequent exposure of physiological signals, e.g. hormones, antibodies, peptides, antigens, cytokines, growth factors, action potentials, pharmacological agents including chemotherapeutics, radiation, carcinogenics, or other cells (i.e., cell-cell contacts). In another example, the determinations are made at different stages of the cell cycle process. In this way, compounds that modulate genes or proteins of the invention are identified. Compounds with pharmacological activity are able to enhance or interfere with the activity of the cancer protein of the invention. Once identified, similar structures are evaluated to identify critical structural features of the compound. 
     In one embodiment, a method of modulating (e.g., inhibiting) cancer cell division is provided; the method comprises administration of a cancer modulator. In another embodiment, a method of modulating (e.g., inhibiting) cancer is provided; the method comprises administration of a cancer modulator. In a further embodiment, methods of treating cells or individuals with cancer are provided; the method comprises administration of a cancer modulator. 
     In one embodiment, a method for modulating the status of a cell that expresses a gene of the invention is provided. As used herein status comprises such art-accepted parameters such as growth, proliferation, survival, function, apoptosis, senescence, location, enzymatic activity, signal transduction, etc. of a cell. In one embodiment, a cancer inhibitor is an antibody as discussed above. In another embodiment, the cancer inhibitor is an antisense molecule. A variety of cell growth, proliferation, and metastasis assays are known to those of skill in the art, as described herein. 
     XIII.E.) High Throughput Screening to Identify Modulators 
     The assays to identify suitable modulators are amenable to high throughput screening. Preferred assays thus detect enhancement or inhibition of cancer gene transcription, inhibition or enhancement of polypeptide expression, and inhibition or enhancement of polypeptide activity. 
     In one embodiment, modulators evaluated in high throughput screening methods are proteins, often naturally occurring proteins or fragments of naturally occurring proteins. Thus, e.g., cellular extracts containing proteins, or random or directed digests of proteinaceous cellular extracts, are used. In this way, libraries of proteins are made for screening in the methods of the invention. Particularly preferred in this embodiment are libraries of bacterial, fungal, viral, and mammalian proteins, with the latter being preferred, and human proteins being especially preferred. Particularly useful test compound will be directed to the class of proteins to which the target belongs, e.g., substrates for enzymes, or ligands and receptors. 
     XIII.F.) Use of Soft Agar Growth and Colony Formation to Identify and Characterize Modulators 
     Normal cells require a solid substrate to attach and grow. When cells are transformed, they lose this phenotype and grow detached from the substrate. For example, transformed cells can grow in stirred suspension culture or suspended in semi-solid media, such as semi-solid or soft agar. The transformed cells, when transfected with tumor suppressor genes, can regenerate normal phenotype and once again require a solid substrate to attach to and grow. Soft agar growth or colony formation in assays are used to identify modulators of cancer sequences, which when expressed in host cells, inhibit abnormal cellular proliferation and transformation. A modulator reduces or eliminates the host cells&#39; ability to grow suspended in solid or semisolid media, such as agar. 
     Techniques for soft agar growth or colony formation in suspension assays are described in Freshney, Culture of Animal Cells a Manual of Basic Technique (3rd ed., 1994). See also, the methods section of Garkavtsev et al. (1996), supra. 
     XIII.G.) Evaluation of Contact Inhibition and Growth Density Limitation to Identify and Characterize Modulators 
     Normal cells typically grow in a flat and organized pattern in cell culture until they touch other cells. When the cells touch one another, they are contact inhibited and stop growing. Transformed cells, however, are not contact inhibited and continue to grow to high densities in disorganized foci. Thus, transformed cells grow to a higher saturation density than corresponding normal cells. This is detected morphologically by the formation of a disoriented monolayer of cells or cells in foci. Alternatively, labeling index with (3H)-thymidine at saturation density is used to measure density limitation of growth, similarly an MTT or Alamar blue assay will reveal proliferation capacity of cells and the ability of modulators to affect same. See Freshney (1994), supra. Transformed cells, when transfected with tumor suppressor genes, can regenerate a normal phenotype and become contact inhibited and would grow to a lower density. 
     In this assay, labeling index with 3H)-thymidine at saturation density is a preferred method of measuring density limitation of growth. Transformed host cells are transfected with a cancer-associated sequence and are grown for 24 hours at saturation density in non-limiting medium conditions. The percentage of cells labeling with (3H)-thymidine is determined by incorporated cpm. 
     Contact independent growth is used to identify modulators of cancer sequences, which had led to abnormal cellular proliferation and transformation. A modulator reduces or eliminates contact independent growth, and returns the cells to a normal phenotype. 
     XIII.H.) Evaluation of Growth Factor or Serum Dependence to Identify and Characterize Modulators 
     Transformed cells have lower serum dependence than their normal counterparts (see, e.g., Temin, J. Natl. Cancer Inst. 37:167-175 (1966); Eagle et al., J. Exp. Med. 131:836-879 (1970)); Freshney, supra. This is in part due to release of various growth factors by the transformed cells. The degree of growth factor or serum dependence of transformed host cells can be compared with that of control. For example, growth factor or serum dependence of a cell is monitored in methods to identify and characterize compounds that modulate cancer-associated sequences of the invention. 
     XIII.I.) Use of Tumor-Specific Marker Levels to Identify and Characterize Modulators 
     Tumor cells release an increased amount of certain factors (hereinafter “tumor specific markers”) than their normal counterparts. For example, plasminogen activator (PA) is released from human glioma at a higher level than from normal brain cells (see, e.g., Gullino, Angiogenesis, Tumor Vascularization, and Potential Interference with Tumor Growth, in Biological Responses in Cancer, pp. 178-184 (Mihich (ed.) 1985)). Similarly, Tumor Angiogenesis Factor (TAF) is released at a higher level in tumor cells than their normal counterparts. See, e.g., Folkman, Angiogenesis and Cancer, Sem Cancer Biol. (1992)), while bFGF is released from endothelial tumors (Ensoli, B et al). 
     Various techniques which measure the release of these factors are described in Freshney (1994), supra. Also, see, Unkless et al., J. Biol. Chem. 249:4295-4305 (1974); Strickland &amp; Beers, J. Biol. Chem. 251:5694-5702 (1976); Whur et al., Br. J. Cancer 42:305 312 (1980); Gullino, Angiogenesis, Tumor Vascularization, and Potential Interference with Tumor Growth, in Biological Responses in Cancer, pp. 178-184 (Mihich (ed.) 1985); Freshney, Anticancer Res. 5:111-130 (1985). For example, tumor specific marker levels are monitored in methods to identify and characterize compounds that modulate cancer-associated sequences of the invention. 
     XIII.J.) Invasiveness into Matrigel to Identify and Characterize Modulators 
     The degree of invasiveness into Matrigel or an extracellular matrix constituent can be used as an assay to identify and characterize compounds that modulate cancer associated sequences. Tumor cells exhibit a positive correlation between malignancy and invasiveness of cells into Matrigel or some other extracellular matrix constituent. In this assay, tumorigenic cells are typically used as host cells. Expression of a tumor suppressor gene in these host cells would decrease invasiveness of the host cells. Techniques described in Cancer Res. 1999; 59:6010; Freshney (1994), supra, can be used. Briefly, the level of invasion of host cells is measured by using filters coated with Matrigel or some other extracellular matrix constituent. Penetration into the gel, or through to the distal side of the filter, is rated as invasiveness, and rated histologically by number of cells and distance moved, or by prelabeling the cells with 1251 and counting the radioactivity on the distal side of the filter or bottom of the dish. See, e.g., Freshney (1984), supra. 
     XIII.K.) Evaluation of Tumor Growth In Vivo to Identify and Characterize Modulators 
     Effects of cancer-associated sequences on cell growth are tested in transgenic or immune-suppressed organisms. Transgenic organisms are prepared in a variety of art-accepted ways. For example, knock-out transgenic organisms, e.g., mammals such as mice, are made, in which a cancer gene is disrupted or in which a cancer gene is inserted. Knock-out transgenic mice are made by insertion of a marker gene or other heterologous gene into the endogenous cancer gene site in the mouse genome via homologous recombination. Such mice can also be made by substituting the endogenous cancer gene with a mutated version of the cancer gene, or by mutating the endogenous cancer gene, e.g., by exposure to carcinogens. 
     To prepare transgenic chimeric animals, e.g., mice, a DNA construct is introduced into the nuclei of embryonic stem cells. Cells containing the newly engineered genetic lesion are injected into a host mouse embryo, which is re-implanted into a recipient female. Some of these embryos develop into chimeric mice that possess germ cells some of which are derived from the mutant cell line. Therefore, by breeding the chimeric mice it is possible to obtain a new line of mice containing the introduced genetic lesion (see, e.g., Capecchi et al., Science 244:1288 (1989)). Chimeric mice can be derived according to U.S. Pat. No. 6,365,797, issued 2 Apr. 2002; U.S. Pat. No. 6,107,540 issued 22 Aug. 2000; Hogan et al., Manipulating the Mouse Embryo: A laboratory Manual, Cold Spring Harbor Laboratory (1988) and Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, Robertson, ed., IRL Press, Washington, D.C., (1987). 
     Alternatively, various immune-suppressed or immune-deficient host animals can be used. For example, a genetically athymic “nude” mouse (see, e.g., Giovanella et al., J. Natl. Cancer Inst. 52:921 (1974)), a SCID mouse, a thymectornized mouse, or an irradiated mouse (see, e.g., Bradley et al., Br. J. Cancer 38:263 (1978); Selby et al., Br. J. Cancer 41:52 (1980)) can be used as a host. Transplantable tumor cells (typically about 106 cells) injected into isogenic hosts produce invasive tumors in a high proportion of cases, while normal cells of similar origin will not. In hosts which developed invasive tumors, cells expressing cancer-associated sequences are injected subcutaneously or orthotopically. Mice are then separated into groups, including control groups and treated experimental groups) e.g. treated with a modulator). After a suitable length of time, preferably 4-8 weeks, tumor growth is measured (e.g., by volume or by its two largest dimensions, or weight) and compared to the control. Tumors that have statistically significant reduction (using, e.g., Student&#39;s T test) are said to have inhibited growth. 
     XIII.L.) In Vitro Assays to Identify and Characterize Modulators 
     Assays to identify compounds with modulating activity can be performed in vitro. For example, a cancer polypeptide is first contacted with a potential modulator and incubated for a suitable amount of time, e.g., from 0.5 to 48 hours. In one embodiment, the cancer polypeptide levels are determined in vitro by measuring the level of protein or mRNA. The level of protein is measured using immunoassays such as Western blotting, ELISA and the like with an antibody that selectively binds to the cancer polypeptide or a fragment thereof. For measurement of mRNA, amplification, e.g., using PCR, LCR, or hybridization assays, e.g., Northern hybridization, RNAse protection, dot blotting, are preferred. The level of protein or mRNA is detected using directly or indirectly labeled detection agents, e.g., fluorescently or radioactively labeled nucleic acids, radioactively or enzymatically labeled antibodies, and the like, as described herein. 
     Alternatively, a reporter gene system can be devised using a cancer protein promoter operably linked to a reporter gene such as luciferase, green fluorescent protein, CAT, or P-gal. The reporter construct is typically transfected into a cell. After treatment with a potential modulator, the amount of reporter gene transcription, translation, or activity is measured according to standard techniques known to those of skill in the art (Davis G F, supra; Gonzalez, J. &amp; Negulescu, P. Curr. Opin. Biotechnol. 1998: 9:624). 
     As outlined above, in vitro screens are done on individual genes and gene products. That is, having identified a particular differentially expressed gene as important in a particular state, screening of modulators of the expression of the gene or the gene product itself is performed. 
     In one embodiment, screening for modulators of expression of specific gene(s) is performed. Typically, the expression of only one or a few genes is evaluated. In another embodiment, screens are designed to first find compounds that bind to differentially expressed proteins. These compounds are then evaluated for the ability to modulate differentially expressed activity. Moreover, once initial candidate compounds are identified, variants can be further screened to better evaluate structure activity relationships. 
     XIII.M.) Binding Assays to Identify and Characterize Modulators 
     In binding assays in accordance with the invention, a purified or isolated gene product of the invention is generally used. For example, antibodies are generated to a protein of the invention, and immunoassays are run to determine the amount and/or location of protein. Alternatively, cells comprising the cancer proteins are used in the assays. 
     Thus, the methods comprise combining a cancer protein of the invention and a candidate compound such as a ligand, and determining the binding of the compound to the cancer protein of the invention. Preferred embodiments utilize the human cancer protein; animal models of human disease of can also be developed and used. Also, other analogous mammalian proteins also can be used as appreciated by those of skill in the art. Moreover, in some embodiments variant or derivative cancer proteins are used. 
     Generally, the cancer protein of the invention, or the ligand, is non-diffusibly bound to an insoluble support. The support can, e.g., be one having isolated sample receiving areas (a microtiter plate, an array, etc.). The insoluble supports can be made of any composition to which the compositions can be bound, is readily separated from soluble material, and is otherwise compatible with the overall method of screening. The surface of such supports can be solid or porous and of any convenient shape. 
     Examples of suitable insoluble supports include microtiter plates, arrays, membranes and beads. These are typically made of glass, plastic (e.g., polystyrene), polysaccharide, nylon, nitrocellulose, or Teflon™, etc. Microtiter plates and arrays are especially convenient because a large number of assays can be carried out simultaneously, using small amounts of reagents and samples. The particular manner of binding of the composition to the support is not crucial so long as it is compatible with the reagents and overall methods of the invention, maintains the activity of the composition and is nondiffusable. Preferred methods of binding include the use of antibodies which do not sterically block either the ligand binding site or activation sequence when attaching the protein to the support, direct binding to “sticky” or ionic supports, chemical crosslinking, the synthesis of the protein or agent on the surface, etc. Following binding of the protein or ligand/binding agent to the support, excess unbound material is removed by washing. The sample receiving areas may then be blocked through incubation with bovine serum albumin (BSA), casein or other innocuous protein or other moiety. 
     Once a cancer protein of the invention is bound to the support, and a test compound is added to the assay. Alternatively, the candidate binding agent is bound to the support and the cancer protein of the invention is then added. Binding agents include specific antibodies, non-natural binding agents identified in screens of chemical libraries, peptide analogs, etc. 
     Of particular interest are assays to identify agents that have a low toxicity for human cells. A wide variety of assays can be used for this purpose, including proliferation assays, cAMP assays, labeled in vitro protein-protein binding assays, electrophoretic mobility shift assays, immunoassays for protein binding, functional assays (phosphorylation assays, etc.) and the like. 
     A determination of binding of the test compound (ligand, binding agent, modulator, etc.) to a cancer protein of the invention can be done in a number of ways. The test compound can be labeled, and binding determined directly, e.g., by attaching all or a portion of the cancer protein of the invention to a solid support, adding a labeled candidate compound (e.g., a fluorescent label), washing off excess reagent, and determining whether the label is present on the solid support. Various blocking and washing steps can be utilized as appropriate. 
     In certain embodiments, only one of the components is labeled, e.g., a protein of the invention or ligands labeled. Alternatively, more than one component is labeled with different labels, e.g., I125, for the proteins and a fluorophor for the compound. Proximity reagents, e.g., quenching or energy transfer reagents are also useful. 
     XIII.N.) Competitive Binding to Identify and Characterize Modulators 
     In one embodiment, the binding of the “test compound” is determined by competitive binding assay with a “competitor.” The competitor is a binding moiety that binds to the target molecule (e.g., a cancer protein of the invention). Competitors include compounds such as antibodies, peptides, binding partners, ligands, etc. Under certain circumstances, the competitive binding between the test compound and the competitor displaces the test compound. In one embodiment, the test compound is labeled. Either the test compound, the competitor, or both, is added to the protein for a time sufficient to allow binding. Incubations are performed at a temperature that facilitates optimal activity, typically between four and 40° C. Incubation periods are typically optimized, e.g., to facilitate rapid high throughput screening; typically between zero and one hour will be sufficient. Excess reagent is generally removed or washed away. The second component is then added, and the presence or absence of the labeled component is followed, to indicate binding. 
     In one embodiment, the competitor is added first, followed by the test compound. Displacement of the competitor is an indication that the test compound is binding to the cancer protein and thus is capable of binding to, and potentially modulating, the activity of the cancer protein. In this embodiment, either component can be labeled. Thus, e.g., if the competitor is labeled, the presence of label in the post-test compound wash solution indicates displacement by the test compound. Alternatively, if the test compound is labeled, the presence of the label on the support indicates displacement. 
     In an alternative embodiment, the test compound is added first, with incubation and washing, followed by the competitor. The absence of binding by the competitor indicates that the test compound binds to the cancer protein with higher affinity than the competitor. Thus, if the test compound is labeled, the presence of the label on the support, coupled with a lack of competitor binding, indicates that the test compound binds to and thus potentially modulates the cancer protein of the invention. 
     Accordingly, the competitive binding methods comprise differential screening to identity agents that are capable of modulating the activity of the cancer proteins of the invention. In this embodiment, the methods comprise combining a cancer protein and a competitor in a first sample. A second sample comprises a test compound, the cancer protein, and a competitor. The binding of the competitor is determined for both samples, and a change, or difference in binding between the two samples indicates the presence of an agent capable of binding to the cancer protein and potentially modulating its activity. That is, if the binding of the competitor is different in the second sample relative to the first sample, the agent is capable of binding to the cancer protein. 
     Alternatively, differential screening is used to identify drug candidates that bind to the native cancer protein, but cannot bind to modified cancer proteins. For example the structure of the cancer protein is modeled and used in rational drug design to synthesize agents that interact with that site, agents which generally do not bind to site-modified proteins. Moreover, such drug candidates that affect the activity of a native cancer protein are also identified by screening drugs for the ability to either enhance or reduce the activity of such proteins. 
     Positive controls and negative controls can be used in the assays. Preferably control and test samples are performed in at least triplicate to obtain statistically significant results. Incubation of all samples occurs for a time sufficient to allow for the binding of the agent to the protein. Following incubation, samples are washed free of non-specifically bound material and the amount of bound, generally labeled agent determined For example, where a radiolabel is employed, the samples can be counted in a scintillation counter to determine the amount of bound compound. 
     A variety of other reagents can be included in the screening assays. These include reagents like salts, neutral proteins, e.g. albumin, detergents, etc. which are used to facilitate optimal protein-protein binding and/or reduce non-specific or background interactions. Also reagents that otherwise improve the efficiency of the assay, such as protease inhibitors, nuclease inhibitors, anti-microbial agents, etc., can be used. The mixture of components is added in an order that provides for the requisite binding. 
     XIII.O.) Use of Polynucleotides to Down-regulate or Inhibit a Protein of the Invention. 
     Polynucleotide modulators of cancer can be introduced into a cell containing the target nucleotide sequence by formation of a conjugate with a ligand-binding molecule, as described in WO 91/04753. Suitable ligand-binding molecules include, but are not limited to, cell surface receptors, growth factors, other cytokines, or other ligands that bind to cell surface receptors. Preferably, conjugation of the ligand binding molecule does not substantially interfere with the ability of the ligand binding molecule to bind to its corresponding molecule or receptor, or block entry of the sense or antisense oligonucleotide or its conjugated version into the cell. Alternatively, a polynucleotide modulator of cancer can be introduced into a cell containing the target nucleic acid sequence, e.g., by formation of a polynucleotide-lipid complex, as described in WO 90/10448. It is understood that the use of antisense molecules or knock out and knock in models may also be used in screening assays as discussed above, in addition to methods of treatment. 
     XIII.P.) Inhibitory and Antisense Nucleotides 
     In certain embodiments, the activity of a cancer-associated protein is down-regulated, or entirely inhibited, by the use of antisense polynucleotide or inhibitory small nuclear RNA (snRNA), i.e., a nucleic acid complementary to, and which can preferably hybridize specifically to, a coding mRNA nucleic acid sequence, e.g., a cancer protein of the invention, mRNA, or a subsequence thereof. Binding of the antisense polynucleotide to the mRNA reduces the translation and/or stability of the mRNA. 
     In the context of this invention, antisense polynucleotides can comprise naturally occurring nucleotides, or synthetic species formed from naturally occurring subunits or their close homologs. Antisense polynucleotides may also have altered sugar moieties or inter-sugar linkages. Exemplary among these are the phosphorothioate and other sulfur containing species which are known for use in the art. Analogs are comprised by this invention so long as they function effectively to hybridize with nucleotides of the invention. See, e.g., Isis Pharmaceuticals, Carlsbad, Calif.; Sequitor, Inc., Natick, Mass. 
     Such antisense polynucleotides can readily be synthesized using recombinant means, or can be synthesized in vitro. Equipment for such synthesis is sold by several vendors, including Applied Biosystems. The preparation of other oligonucleotides such as phosphorothioates and alkylated derivatives is also well known to those of skill in the art. 
     Antisense molecules as used herein include antisense or sense oligonucleotides. Sense oligonucleotides can, e.g., be employed to block transcription by binding to the anti-sense strand. The antisense and sense oligonucleotide comprise a single stranded nucleic acid sequence (either RNA or DNA) capable of binding to target mRNA (sense) or DNA (antisense) sequences for cancer molecules. Antisense or sense oligonucleotides, according to the present invention, comprise a fragment generally at least about 12 nucleotides, preferably from about 12 to 30 nucleotides. The ability to derive an antisense or a sense oligonucleotide, based upon a cDNA sequence encoding a given protein is described in, e.g., Stein &amp;Cohen (Cancer Res. 48:2659 (1988 and van der Krol et al. (BioTechniques 6:958 (1988)). 
     XIII.Q.) Ribozymes 
     In addition to antisense polynucleotides, ribozymes can be used to target and inhibit transcription of cancer-associated nucleotide sequences. A ribozyme is an RNA molecule that catalytically cleaves other RNA molecules. Different kinds of ribozymes have been described, including group I ribozymes, hammerhead ribozymes, hairpin ribozymes, RNase P, and axhead ribozymes (see, e.g., Castanotto et al., Adv. in Pharmacology 25: 289-317 (1994) for a general review of the properties of different ribozymes). 
     The general features of hairpin ribozymes are described, e.g., in Hampel et al., Nucl. Acids Res. 18:299-304 (1990); European Patent Publication No. 0360257; U.S. Pat. No. 5,254,678. Methods of preparing are well known to those of skill in the art (see, e.g., WO 94/26877; Ojwang et al., Proc. Natl. Acad. Sci. USA 90:6340-6344 (1993); Yamada et al., Human Gene Therapy 1:39-45 (1994); Leavitt et al., Proc. Natl. Acad. Sci. USA 92:699-703 (1995); Leavitt et al., Human Gene Therapy 5: 1151-120 (1994); and Yamada et al., Virology 205: 121-126 (1994)). 
     XIII.R.) Use of Modulators in Phenotypic Screening 
     In one embodiment, a test compound is administered to a population of cancer cells, which have an associated cancer expression profile. By “administration” or “contacting” herein is meant that the modulator is added to the cells in such a manner as to allow the modulator to act upon the cell, whether by uptake and intracellular action, or by action at the cell surface. In some embodiments, a nucleic acid encoding a proteinaceous agent (i.e., a peptide) is put into a viral construct such as an adenoviral or retroviral construct, and added to the cell, such that expression of the peptide agent is accomplished, e.g., PCT US97/01019. Regulatable gene therapy systems can also be used. Once the modulator has been administered to the cells, the cells are washed if desired and are allowed to incubate under preferably physiological conditions for some period. The cells are then harvested and a new gene expression profile is generated. Thus, e.g., cancer tissue is screened for agents that modulate, e.g., induce or suppress, the cancer phenotype. A change in at least one gene, preferably many, of the expression profile indicates that the agent has an effect on cancer activity. Similarly, altering a biological function or a signaling pathway is indicative of modulator activity. By defining such a signature for the cancer phenotype, screens for new drugs that alter the phenotype are devised. With this approach, the drug target need not be known and need not be represented in the original gene/protein expression screening platform, nor does the level of transcript for the target protein need to change. The modulator inhibiting function will serve as a surrogate marker. 
     As outlined above, screens are done to assess genes or gene products. That is, having identified a particular differentially expressed gene as important in a particular state, screening of modulators of either the expression of the gene or the gene product itself is performed. 
     XIII.S.) Use of Modulators to Affect Peptides of the Invention 
     Measurements of cancer polypeptide activity, or of the cancer phenotype are performed using a variety of assays. For example, the effects of modulators upon the function of a cancer polypeptide(s) are measured by examining parameters described above. A physiological change that affects activity is used to assess the influence of a test compound on the polypeptides of this invention. When the functional outcomes are determined using intact cells or animals, a variety of effects can be assesses such as, in the case of a cancer associated with solid tumors, tumor growth, tumor metastasis, neovascularization, hormone release, transcriptional changes to both known and uncharacterized genetic markers (e.g., by Northern blots), changes in cell metabolism such as cell growth or pH changes, and changes in intracellular second messengers such as cGNIP. 
     XIII.T.) Methods of Identifying Characterizing Cancer-Associated Sequences 
     Expression of various gene sequences is correlated with cancer. Accordingly, disorders based on mutant or variant cancer genes are determined. In one embodiment, the invention provides methods for identifying cells containing variant cancer genes, e.g., determining the presence of, all or part, the sequence of at least one endogenous cancer gene in a cell. This is accomplished using any number of sequencing techniques. The invention comprises methods of identifying the cancer genotype of an individual, e.g., determining all or part of the sequence of at least one gene of the invention in the individual. This is generally done in at least one tissue of the individual, e.g., a tissue set forth in Table I, and may include the evaluation of a number of tissues or different samples of the same tissue. The method may include comparing the sequence of the sequenced gene to a known cancer gene, i.e., a wild-type gene to determine the presence of family members, homologies, mutations or variants. The sequence of all or part of the gene can then be compared to the sequence of a known cancer gene to determine if any differences exist. This is done using any number of known homology programs, such as BLAST, Bestfit, etc. The presence of a difference in the sequence between the cancer gene of the patient and the known cancer gene correlates with a disease state or a propensity for a disease state, as outlined herein. 
     In a preferred embodiment, the cancer genes are used as probes to determine the number of copies of the cancer gene in the genome. The cancer genes are used as probes to determine the chromosomal localization of the cancer genes. Information such as chromosomal localization finds use in providing a diagnosis or prognosis in particular when chromosomal abnormalities such as translocations, and the like are identified in the cancer gene locus. 
     XIV.) Kits/Articles of Manufacture 
     For use in the laboratory, prognostic, prophylactic, diagnostic and therapeutic applications described herein, kits are within the scope of the invention. Such kits can comprise a carrier, package, or container that is compartmentalized to receive one or more containers such as vials, tubes, and the like, each of the container(s) comprising one of the separate elements to be used in the method, along with a label or insert comprising instructions for use, such as a use described herein. For example, the container(s) can comprise a probe that is or can be detectably labeled. Such probe can be an antibody or polynucleotide specific for a protein or a gene or message of the invention, respectively. Where the method utilizes nucleic acid hybridization to detect the target nucleic acid, the kit can also have containers containing nucleotide(s) for amplification of the target nucleic acid sequence. Kits can comprise a container comprising a reporter, such as a biotin-binding protein, such as avidin or streptavidin, bound to a reporter molecule, such as an enzymatic, fluorescent, or radioisotope label; such a reporter can be used with, e.g., a nucleic acid or antibody. The kit can include all or part of the amino acid sequences in  FIG. 2  or  FIG. 3  or analogs thereof, or a nucleic acid molecule that encodes such amino acid sequences. 
     The kit of the invention will typically comprise the container described above and one or more other containers associated therewith that comprise materials desirable from a commercial and user standpoint, including buffers, diluents, filters, needles, syringes; carrier, package, container, vial and/or tube labels listing contents and/or instructions for use, and package inserts with instructions for use. 
     A label can be present on or with the container to indicate that the composition is used for a specific therapy or non-therapeutic application, such as a prognostic, prophylactic, diagnostic or laboratory application, and can also indicate directions for either in vivo or in vitro use, such as those described herein. Directions and or other information can also be included on an insert(s) or label(s) which is included with or on the kit. The label can be on or associated with the container. A label a can be on a container when letters, numbers or other characters forming the label are molded or etched into the container itself; a label can be associated with a container when it is present within a receptacle or carrier that also holds the container, e.g., as a package insert. The label can indicate that the composition is used for diagnosing, treating, prophylaxing or prognosing a condition, such as a neoplasia of a tissue set forth in Table I. 
     The terms “kit” and “article of manufacture” can be used as synonyms. 
     In another embodiment of the invention, an article(s) of manufacture containing compositions, such as amino acid sequence(s), small molecule(s), nucleic acid sequence(s), and/or antibody(s), e.g., materials useful for the diagnosis, prognosis, prophylaxis and/or treatment of neoplasias of tissues such as those set forth in Table I is provided. The article of manufacture typically comprises at least one container and at least one label. Suitable containers include, for example, bottles, vials, syringes, and test tubes. The containers can be formed from a variety of materials such as glass, metal or plastic. The container can hold amino acid sequence(s), small molecule(s), nucleic acid sequence(s), cell population(s) and/or antibody(s). In one embodiment, the container holds a polynucleotide for use in examining the mRNA expression profile of a cell, together with reagents used for this purpose. In another embodiment a container comprises an antibody, binding fragment thereof or specific binding protein for use in evaluating protein expression of 158P3D2 in cells and tissues, or for relevant laboratory, prognostic, diagnostic, prophylactic and therapeutic purposes; indications and/or directions for such uses can be included on or with such container, as can reagents and other compositions or tools used for these purposes. In another embodiment, a container comprises materials for eliciting a cellular or humoral immune response, together with associated indications and/or directions. In another embodiment, a container comprises materials for adoptive immunotherapy, such as cytotoxic T cells (CTL) or helper T cells (HTL), together with associated indications and/or directions; reagents and other compositions or tools used for such purpose can also be included. 
     The container can alternatively hold a composition that is effective for treating, diagnosis, prognosing or prophylaxing a condition and can have a sterile access port (for example the container can be an intravenous solution bag or a vial having a stopper pierceable by a hypodermic injection needle). The active agents in the composition can be an antibody capable of specifically binding 158P3D2 and modulating the function of 158P3D2. 
     The article of manufacture can further comprise a second container comprising a pharmaceutically-acceptable buffer, such as phosphate-buffered saline, Ringer&#39;s solution and/or dextrose solution. It can further include other materials desirable from a commercial and user standpoint, including other buffers, diluents, filters, stirrers, needles, syringes, and/or package inserts with indications and/or instructions for use. 
     EXAMPLES 
     Various aspects of the invention are further described and illustrated by way of the several examples that follow, none of which is intended to limit the scope of the invention. 
     Example 1 
     SSH-Generated Isolation of a cDNA Fragment of the 158P3D2 Gene 
     To isolate genes that are over-expressed in bladder cancer we used the Suppression Subtractive Hybridization (SSH) procedure using cDNA derived from bladder cancer tissues, including invasive transitional cell carcinoma. The 158P3D2 SSH cDNA sequence was derived from a bladder cancer pool minus normal bladder cDNA subtraction. Included in the driver were also cDNAs derived from 9 other normal tissues. The 158P3D2 cDNA was identified as highly expressed in the bladder cancer tissue pool, with lower expression seen in a restricted set of normal tissues. 
     The SSH DNA sequence of 312 bp ( FIG. 1 ) shows identity to the fer-1-like 4 ( C. elegans ) (FER1L4) mRNA. A 158P3D2 cDNA clone 158P3D2-BCP1 of 1994 bp was isolated from bladder cancer cDNA, revealing an ORF of 328 amino acids ( FIG. 2 ,  FIG. 3 ). 
     Amino acid sequence analysis of 158P3D2 reveals 100% identity over 328 amino acid region to dJ477O4.1.1, a novel protein similar to otoferlin and dysferlin, isoform 1 protein (GenBank Accession CAB89410.1). 
     The 158P3D2 protein has a transmembrane domain of 23 residues between amino acids 292-313 predicted by the SOSUI Signal program. 
     Materials and Methods 
     Human Tissues: 
     The patient cancer and normal tissues were purchased from different sources such as the NDRI (Philadelphia, Pa.). mRNA for some of the normal tissues were purchased from Clontech, Palo Alto, Calif. 
     RNA Isolation: 
     Tissues were homogenized in Trizol reagent (Life Technologies, Gibco BRL) using 10 ml/g tissue isolate total RNA. Poly A RNA was purified from total RNA using Qiagen&#39;s Oligotex mRNA Mini and Midi kits. Total and mRNA were quantified by spectrophotometric analysis (O.D. 260/280 nm) and analyzed by gel electrophoresis. 
     Oligonucleotides: 
     The following HPLC purified oligonucleotides were used. 
     
       
         
           
               
               
            
               
                   
                 DPNCDN (cDNA synthesis primer): 
               
               
                   
                 (SEQ ID NO: 67) 
               
               
                   
                 5′TTTT GATC AAGCTT 30 3′ 
               
               
                   
                   
               
               
                   
                 Adaptor 1: 
               
               
                   
                 (SEQ ID NO: 68) 
               
               
                   
                 5′CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAG3′ 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 69) 
               
               
                   
                 3′GGCCCGTC CTAG 5′ 
               
               
                   
                   
               
               
                   
                 Adaptor 2: 
               
               
                   
                 (SEQ ID NO: 70) 
               
               
                   
                 5′GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG3′ 
               
               
                   
                   
               
               
                   
                 (SEQ ID NO: 71) 
               
               
                   
                 3′CGGCTC CTAG 5′ 
               
               
                   
                   
               
               
                   
                 PCR primer 1: 
               
               
                   
                 (SEQ ID NO: 72) 
               
               
                   
                 5′CTAATACGACTCACTATAGGGC3′ 
               
               
                   
                   
               
               
                   
                 Nested primer (NP)1: 
               
               
                   
                 (SEQ ID NO: 73) 
               
               
                   
                 5′TCGAGCGGCCGCCCGGGCAG GA 3′ 
               
               
                   
                   
               
               
                   
                 Nested primer (NP)2: 
               
               
                   
                 (SEQ ID NO: 74) 
               
               
                   
                 5′AGCGTGGTCGCGGCCGAG GA 3′ 
               
            
           
         
       
     
     Suppression Subtractive Hybridization: 
     Suppression Subtractive Hybridization (SSH) was used to identify cDNAs corresponding to genes that may be differentially expressed in bladder cancer. The SSH reaction utilized cDNA from bladder cancer and normal tissues. 
     The gene 158P3D2 sequence was derived from a bladder cancer pool minus normal bladder cDNA subtraction. The SSH DNA sequence ( FIG. 1 ) was identified. 
     The cDNA derived from of pool of normal bladder tissues was used as the source of the “driver” cDNA, while the cDNA from a pool of bladder cancer tissues was used as the source of the “tester” cDNA. Double stranded cDNAs corresponding to tester and driver cDNAs were synthesized from 2 μg of poly(A) +  RNA isolated from the relevant xenograft tissue, as described above, using CLONTECH&#39;s PCR-Select cDNA Subtraction Kit and 1 ng of oligonucleotide DPNCDN as primer. First- and second-strand synthesis were carried out as described in the Kit&#39;s user manual protocol (CLONTECH Protocol No. PT1117-1, Catalog No. K1804-1). The resulting cDNA was digested with Dpn II for 3 hrs at 37° C. Digested cDNA was extracted with phenol/chloroform (1:1) and ethanol precipitated. 
     Driver cDNA was generated by combining in a 1:1 ratio Dpn II digested cDNA from the relevant tissue source (see above) with a mix of digested cDNAs derived from the nine normal tissues: stomach, skeletal muscle, lung, brain, liver, kidney, pancreas, small intestine, and heart. 
     Tester cDNA was generated by diluting 1 μl of Dpn II digested cDNA from the relevant tissue source (see above) (400 ng) in 5 μl of water. The diluted cDNA (2 μl, 160 ng) was then ligated to 2 μl of Adaptor 1 and Adaptor 2 (10 μM), in separate ligation reactions, in a total volume of 10 μl at 16° C. overnight, using 400 u of T4 DNA ligase (CLONTECH). Ligation was terminated with 1 μl of 0.2 M EDTA and heating at 72° C. for 5 min 
     The first hybridization was performed by adding 1.5 μl (600 ng) of driver cDNA to each of two tubes containing 1.5 μl (20 ng) Adaptor 1- and Adaptor 2-ligated tester cDNA. In a final volume of 4 μl, the samples were overlaid with mineral oil, denatured in an MJ Research thermal cycler at 98° C. for 1.5 minutes, and then were allowed to hybridize for 8 hrs at 68° C. The two hybridizations were then mixed together with an additional 1 μl of fresh denatured driver cDNA and were allowed to hybridize overnight at 68° C. The second hybridization was then diluted in 200 μl of 20 mM Hepes, pH 8.3, 50 mM NaCl, 0.2 mM EDTA, heated at 70° C. for 7 min. and stored at −20° C. 
     PCR Amplification, Cloning and Sequencing of Gene Fragments Generated from SSH: 
     To amplify gene fragments resulting from SSH reactions, two PCR amplifications were performed. In the primary PCR reaction 1 μl of the diluted final hybridization mix was added to 1 μl of PCR primer 1 (10 μM), 0.5 μl dNTP mix (10 μM), 2.5 μl 10× reaction buffer (CLONTECH) and 0.5 μl 50× Advantage cDNA polymerase Mix (CLONTECH) in a final volume of 254 PCR 1 was conducted using the following conditions: 75° C. for 5 mM, 94° C. for 25 sec., then 27 cycles of 94° C. for 10 sec, 66° C. for 30 sec, 72° C. for 1.5 mM Five separate primary PCR reactions were performed for each experiment. The products were pooled and diluted 1:10 with water. For the secondary PCR reaction, 1 μl from the pooled and diluted primary PCR reaction was added to the same reaction mix as used for PCR 1, except that primers NP1 and NP2 (10 μM) were used instead of PCR primer 1. PCR 2 was performed using 10-12 cycles of 94° C. for 10 sec, 68° C. for 30 sec, and 72° C. for 1.5 minutes. The PCR products were analyzed using 2% agarose gel electrophoresis. 
     The PCR products were inserted into pCR2.1 using the T/A vector cloning kit (Invitrogen). Transformed  E. coli  were subjected to blue/white and ampicillin selection. White colonies were picked and arrayed into 96 well plates and were grown in liquid culture overnight. To identify inserts, PCR amplification was performed on 1 ml of bacterial culture using the conditions of PCR1 and NP1 and NP2 as primers. PCR products were analyzed using 2% agarose gel electrophoresis. 
     Bacterial clones were stored in 20% glycerol in a 96 well format. Plasmid DNA was prepared, sequenced, and subjected to nucleic acid homology searches of the GenBank, dBest, and NCI-CGAP databases. 
     RT-PCR Expression Analysis: 
     First strand cDNAs can be generated from 1 μg of mRNA with oligo (dT)12-18 priming using the Gibco-BRL Superscript Preamplification system. The manufacturer&#39;s protocol was used which included an incubation for 50 mM at 42° C. with reverse transcriptase followed by RNAse H treatment at 37° C. for 20 mM After completing the reaction, the volume can be increased to 200 μl with water prior to normalization. First strand cDNAs from 16 different normal human tissues can be obtained from Clontech. 
     Normalization of the first strand cDNAs from multiple tissues was performed by using the primers 5′ atatcgccgcgctcgtcgtcgacaa3′ (SEQ ID NO:75) and 5′ agccacacgcagctcattgtagaagg 3′ (SEQ ID NO:76) to amplify β-actin. First strand cDNA (5 μl) were amplified in a total volume of 50 μl containing 0.4 μM primers, 0.2 μM each dNTPs, 1×PCR buffer (Clontech, 10 mM Tris-HCL, 1.5 mM MgCl 2 , 50 mM KCl, pH8.3) and 1× Klentaq DNA polymerase (Clontech). Five μl of the PCR reaction can be removed at 18, 20, and 22 cycles and used for agarose gel electrophoresis. PCR was performed using an MJ Research thermal cycler under the following conditions: Initial denaturation can be at 94° C. for 15 sec, followed by a 18, 20, and 22 cycles of 94° C. for 15, 65° C. for 2 mM, 72° C. for 5 sec. A final extension at 72° C. was carried out for 2 mM After agarose gel electrophoresis, the band intensities of the 283 b.p. β-actin bands from multiple tissues were compared by visual inspection. Dilution factors for the first strand cDNAs were calculated to result in equal β-actin band intensities in all tissues after 22 cycles of PCR. Three rounds of normalization can be required to achieve equal band intensities in all tissues after 22 cycles of PCR. 
     To determine expression levels of the 158P3D2 gene, 5 μl of normalized first strand cDNA were analyzed by PCR using 26, and 30 cycles of amplification. Semi-quantitative expression analysis can be achieved by comparing the PCR products at cycle numbers that give light band intensities. The primers used for RT-PCR were designed using the 158P3D2 SSH sequence and are listed below: 
     
       
         
           
               
               
            
               
                   
                 158P3D2.1 
               
               
                   
                 (SEQ ID NO: 77) 
               
               
                   
                 5′ CATCTATGTGAAGAGCTGGGTGAA 3′ 
               
               
                   
                   
               
               
                   
                 158P3D2.2 
               
               
                   
                 (SEQ ID NO: 78) 
               
               
                   
                 5′ AGGTAGTCAAAGCGGAACACAAAG 3′ 
               
            
           
         
       
     
     Additional primers were also designed to test for expression of the different splice variants and these are listed below: 
     
       
         
           
               
               
             
               
                   
               
               
                 Primer Name 
                 Sequence 
               
               
                   
               
             
            
               
                 158P3D2 ex. 17-R 
                 GTCCTCCCAGCAACTCCACACA 
               
               
                   
                 (SEQ ID NO: 79) 
               
               
                   
               
               
                 158P3D2 ex. 26-F 
                 TGTCCCTTCCACCCAACGTGTGC 
               
               
                   
                 (SEQ ID NO: 80) 
               
               
                   
               
               
                 158P3D2 ex. 28-R 
                 TCCTCCATCTCTCCTTCCTCCTCAG 
               
               
                   
                 (SEQ ID NO: 81) 
               
               
                   
               
               
                 158P3D2 ex. 9-F 
                 CAGAAACTGGTGGGAGTCAACA 
               
               
                   
                 (SEQ ID NO: 82) 
               
               
                   
               
               
                 158P3D2 ex.1-F 
                 ATGGCTCTGACGGTAAGCGTGC 
               
               
                   
                 (SEQ ID NO: 83) 
               
               
                   
               
               
                 158P3D2 ex.10-F 
                 ATAGGCACCTTCAGGATGGACC 
               
               
                   
                 (SEQ ID NO: 84) 
               
               
                   
               
               
                 158P3D2 ex.10-R 
                 TCCATCCTGAAGGTGCCTATCC 
               
               
                   
                 (SEQ ID NO: 85) 
               
               
                   
               
               
                 158P3D2 ex.16-F 
                 CAGAGGAGGAGAAAGAGGAGG 
               
               
                   
                 (SEQ ID NO: 86) 
               
               
                   
               
               
                 158P3D2 ex.16-R 
                 TCCTCTTTCTCCTCCTCTGG 
               
               
                   
                 (SEQ ID NO: 87) 
               
               
                   
               
               
                 158P3D2 ex.21-F 
                 AGATCCAGAGTCTAATGCTCACG 
               
               
                   
                 (SEQ ID NO: 88) 
               
               
                   
               
               
                 158P3D2 ex.21-R 
                 CGTGAGCATTAGACTCTGGATC 
               
               
                   
                 (SEQ ID NO: 89) 
               
               
                   
               
               
                 158P3D2 ex.27-F 
                 AAGGTGTGGAGTCTGAGGTC 
               
               
                   
                 (SEQ ID NO: 90) 
               
               
                   
               
               
                 158P3D2 ex.27-R 
                 ACCTCAGACTCCACACCTTGC 
               
               
                   
                 (SEQ ID NO: 91) 
               
               
                   
               
               
                 158P3D2 ex.34-R 
                 ACTCTGACCAGGAGCTTGATG 
               
               
                   
                 (SEQ ID NO: 92) 
               
               
                   
               
               
                 158P3D2 ex.40-F 
                 ACACGGAGGATGTGGTTCTGG 
               
               
                   
                 (SEQ ID NO: 93) 
               
               
                   
               
               
                 158P3D2 ex.43-F 
                 TTGAGCTGCTGACTGTGGAGGAG 
               
               
                   
                 (SEQ ID NO: 94) 
               
               
                   
               
               
                 158P3D2 ex.43-R 
                 TCCTCCACAGTCAGCAGCTC 
               
               
                   
                 (SEQ ID NO: 95) 
               
               
                   
               
               
                 158P3D2 ex.44-R 
                 TGAGTGTCCAAGGTCAGCGAG 
               
               
                   
                 (SEQ ID NO: 96) 
               
               
                   
               
               
                 158P3D2 ex.7-F 
                 AGAGAATGAGCTGGAGCTTGAGC 
               
               
                   
                 (SEQ ID NO: 97) 
               
               
                   
               
               
                 158P3D2 ex.7-R 
                 TCAAGCTCCAGCTCATTCTCTTC 
               
               
                   
                 (SEQ ID NO: 98) 
               
               
                   
               
               
                 AGS-25 long RT PCR-3′ 
                 TAACACCAGAAAGTTCCACGTCAG 
               
               
                   
                 (SEQ ID NO: 99) 
               
               
                   
               
               
                 AGS-25 long RT PCR-5′ 
                 TGACGGTCGCCGTATTTGATC 
               
               
                   
                 (SEQ ID NO: 100) 
               
               
                   
               
               
                 AGS-25 short RT PCR-3′ 
                 GATTGGCTGCCGAGGCTTGA 
               
               
                   
                 (SEQ ID NO: 101) 
               
               
                   
               
               
                 AGS-25 short RT PCR-5′ 
                 TGACGGTCGCCGTATTTGATC 
               
               
                   
                 (SEQ ID NO: 102) 
               
               
                   
               
            
           
         
       
     
     A typical RT-PCR expression analysis is shown in  FIG. 14 . RT-PCR expression analysis was performed on first strand cDNAs generated using pools of tissues from multiple samples. The cDNAs were shown to be normalized using beta-actin PCR. Results show strong expression of 158P3D2 in bladder cancer pool, kidney cancer pool and cancer metastasis pool. Expression of 158P3D2 is also detected in colon cancer pool, lung cancer pool, ovary cancer pool, breast cancer pool, pancreas cancer pool and prostate metastases to lymph node, and vital pool 2, but not vital pool 1. 
     Example 2 
     Full Length Cloning of 158P3D2 
     The 158P3D2 SSH cDNA sequence was derived from a bladder cancer pool minus normal bladder cDNA subtraction. The SSH cDNA sequence ( FIG. 1 ) was designated 158P3D2. The full-length cDNA clone 158P3D2 v.1 clone 158P3D2-BCP1 and 158P3D2-BCP2 ( FIG. 2 ) were cloned from bladder cancer pool cDNA. 
     Additional 158P3D2 splice and SNP variants have been identified and these are listed in  FIG. 2  and  FIG. 3 . 
     Example 3 
     Chromosomal Mapping of 158P3D2 
     Chromosomal localization can implicate genes in disease pathogenesis. Several chromosome mapping approaches are available including fluorescent in situ hybridization (FISH), human/hamster radiation hybrid (RH) panels (Walter et al., 1994; Nature Genetics 7:22; Research Genetics, Huntsville Ala.), human-rodent somatic cell hybrid panels such as is available from the Coriell Institute (Camden, N.J.), and genomic viewers utilizing BLAST homologies to sequenced and mapped genomic clones (NCBI, Bethesda, Md.). 
     158P3D2 maps to chromosome 8, using 158P3D2 sequence and the NCBI BLAST tool located on the World Wide Web at: (ncbi.nlm.nih.gov/genome/seq/page.cgi?F=HsBlast.html&amp;&amp;ORG=Hs). 
     Example 4 
     Expression Analysis of 158P3D2 in Normal Tissues and Patient Specimens 
     Expression analysis by RT-PCR demonstrated that 158P3D2 is strongly expressed in multiple cancer patient specimens, but unrestricted normal tissues ( FIG. 14 ). First strand cDNA was prepared from a panel of 13 normal tissues (brain, heart, kidney, liver, lung, spleen, skeletal muscle, testis, pancreas, colon, stomach) and pools of 4-7 patients from the following cancer indications: bladder, kidney, colon, lung, pancreas, stomach, ovary, breast, multiple cancer metastasis, cervix, lymphoma as well as from a pool of patient-derived xenografts (prostate cancer, bladder cancer and kidney cancer). Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Samples were run on an agarose gel, and PCR products were quantitated using the AlphaImager software. Results show strong expression of 158P3D2 in cancers of the bladder, kidney, colon, lung, pancreas, stomach, ovary, breast, cervix, and lymphoma. Strong expression was also observed in the cancer metastasis pool. Low expression was detected in all normal tissues tested except in normal stomach. 
     Expression of 158P3D2 in bladder cancer patient specimens and human normal tissues is shown in  FIG. 15 . First strand cDNA was prepared from normal bladder, bladder cancer cell lines (UM-UC-3, TCCSUP, J82) and a panel of bladder cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show expression of 158P3D2 in the majority of bladder cancer patient specimens tested. Very low expression was detected in normal tissues, but no expression was seen in the cell lines tested. 
     Northern blot analysis of 158P3D2 in bladder specimens is shown in  FIG. 16 . RNA was extracted from normal bladder, bladder cancer cell lines (UM-UC-3, J82, SCaBER), bladder cancer patient tumors (T) and their normal adjacent tissues (NAT). Northern blot with 10 μg of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor tissues, but not in normal, nor NAT tissues. 
       FIG. 17  shows 158P3D2 expression in lung cancer patient specimens. First strand cDNA was prepared from normal lung, cancer cell line A427 and a panel of lung cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). 158P3D2 is expressed at varying levels in 35/39 (90%) of lung cancer specimens, but not in all 3 normal lung tissues tested. 
     Northern blot analysis of 158P3D2 expression in lung cancer patient specimens is shown in  FIG. 18 . RNA was extracted from normal lung, A427 lung cancer cell line, and a panel of lung cancer patient specimens. Northern blot with 10 μg of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor specimens but not in normal tissues. 
       FIG. 19  shows 158P3D2 expression in cancer metastasis patient specimens. First strand cDNA was prepared from normal colon, kidney, liver, lung, pancreas, stomach and from a panel of cancer metastasis patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show expression of 158P3D2 in the majority of patient cancer metastasis specimens tested but not in normal tissues. 
       FIG. 20  shows 158P3D2 expression in cervical cancer patient specimens. First strand cDNA was prepared from normal cervix, cervical cancer cell line HeLa, and a panel of cervical cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show expression of 158P3D2 in all 14 cervical cancer patient specimens tested. No expression was detected in normal cervix or in the cell line tested. 
     Northern blot analysis of 158P3D2 expression in cervical cancer patient specimens is shown in  FIG. 21 . RNA was extracted from normal cervix, cervical cancer cell line HeLa, and a panel of cervical cancer patient specimens. Northern blot with 10 μg of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor tissues, but not in normal cervix nor in the cell line. 
       FIG. 22  shows 158P3D2 expression in kidney cancer patient specimens. First strand cDNA was prepared from normal kidney, kidney cancer cell lines (769-P, A-498, CAM-1), and a panel of kidney cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). 158P3D2 is expressed at varying levels in the majority of kidney cancer patient specimens, but not in all 3 normal kidney tissues tested. Low expression was detected in 2 of 3 cell lines tested. 
       FIG. 23  shows 158P3D2 expression in kidney cancer patient specimens by northern blotting. RNA was extracted from normal kidney and a panel of kidney cancer patient specimens. Northern blot with 10 μg of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor specimens but not in the normal tissue. 
       FIG. 24  shows 158P3D2 expression in stomach cancer patient specimens. First strand cDNA was prepared from normal stomach, and a panel of stomach cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). 158P3D2 is expressed at varying levels in the majority of stomach cancer patient specimens. Weak expression was detected in the 2 normal stomach, and only in 1 of the 2 NAT tissues tested. 
       FIG. 25  shows 158P3D2 expression in stomach cancer patient specimens by northern blotting. RNA was extracted from normal stomach and a panel of stomach cancer patient specimens. Northern blot with 10 μg of total RNA were probed with the 158P3D2 sequence. Size standards in kilobases are on the side. Results show strong expression of 158P3D2 in tumor specimens but not in the normal tissue. 
       FIG. 26  shows 158P3D2 expression in colon cancer patient specimens. First strand cDNA was prepared from normal colon, colon cancer cell lines (LoVo, CaCO-2, SK CO 1, Colo 205, T284), and a panel of colon cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×s), medium (signal detected at 26×), high (strong signal at 26×). 158P3D2 is expressed at varying levels in the majority of colon cancer patient specimens. But it was weakly expressed in just 2 of 3 normal tissues, and 3 of 5 cell lines tested. 
       FIG. 27  shows 158P3D2 expression in uterus cancer patient specimens. First strand cDNA was prepared from normal uterus and a panel of uterus cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show 158P3D2 is expressed at varying levels in the majority of uterus cancer patient specimens, but not in normal uterus. 
       FIG. 28  shows 158P3D2 expression in breast cancer patient specimens. First strand cDNA was prepared from normal breast, breast cancer cell lines (MD-MBA-435S, DU4475, MCF-7, CAMA-1, MCF10A), and a panel of breast cancer patient specimens. Normalization was performed by PCR using primers to actin and GAPDH. Semi-quantitative PCR, using primers to 158P3D2, was performed at 26 and 30 cycles of amplification. Expression level was recorded as no expression (no signal detected), low (signal detected at 30×), medium (signal detected at 26×), high (strong signal at 26×). Results show 158P3D2 is expressed at varying levels in the majority of breast cancer patient specimens. But it was weakly expressed in just 2 of 3 normal tissues, and 2 of 5 cell lines tested. 
     The restricted expression of 158P3D2 in normal tissues and the expression detected in bladder cancer, kidney cancer, colon cancer, lung cancer, pancreas cancer, stomach cancer, ovary cancer, breast cancer, uterus cancer, cervical cancer and lymphoma suggest that 158P3D2 is a potential therapeutic target and a diagnostic marker for the treatment of human cancers. 
     Example 5 
     Transcript Variants of 158P3D2 
     Transcript variants are variants of mature mRNA from the same gene which arise by alternative transcription or alternative splicing. Alternative transcripts are transcripts from the same gene but start transcription at different points. Splice variants are mRNA variants spliced differently from the same transcript. In eukaryotes, when a multi-exon gene is transcribed from genomic DNA, the initial RNA is spliced to produce functional mRNA, which has only exons and is used for translation into an amino acid sequence. Accordingly, a given gene can have zero to many alternative transcripts and each transcript can have zero to many splice variants. Each transcript variant has a unique exon makeup, and can have different coding and/or non-coding (5′ or 3′ end) portions, from the original transcript. Transcript variants can code for similar or different proteins with the same or a similar function or can encode proteins with different functions, and can be expressed in the same tissue at the same time, or in different tissues at the same time, or in the same tissue at different times, or in different tissues at different times. Proteins encoded by transcript variants can have similar or different cellular or extracellular localizations, e.g., secreted versus intracellular. 
     Transcript variants are identified by a variety of art-accepted methods. For example, alternative transcripts and splice variants are identified by full-length cloning experiment, or by use of full-length transcript and EST sequences. First, all human ESTs were grouped into clusters which show direct or indirect identity with each other. Second, ESTs in the same cluster were further grouped into sub-clusters and assembled into a consensus sequence. The original gene sequence is compared to the consensus sequence(s) or other full-length sequences. Each consensus sequence is a potential splice variant for that gene. Even when a variant is identified that is not a full-length clone, that portion of the variant is very useful for antigen generation and for further cloning of the full-length splice variant, using techniques known in the art. 
     Moreover, computer programs are available in the art that identify transcript variants based on genomic sequences. Genomic-based transcript variant identification programs include FgenesH (A. Salamov and V. Solovyev, “Ab initio gene finding in  Drosophila  genomic DNA,” Genome Research. 2000 April; 10(4):516-22); Grail (URL compbio.ornl.gov/Grail-bin/EmptyGrailForm) and GenScan (URL genes.mit.edu/GENSCAN.html). For a general discussion of splice variant identification protocols see., e.g., Southan, C., A genomic perspective on human proteases, FEBS Lett. 2001 Jun. 8; 498(2-3):214-8; de Souza, S. J., et al., Identification of human chromosome 22 transcribed sequences with ORF expressed sequence tags, Proc. Natl. Acad Sci USA. 2000 Nov. 7; 97(23):12690-3. 
     To further confirm the parameters of a transcript variant, a variety of techniques are available in the art, such as full-length cloning, proteomic validation, PCR-based validation, and 5′ RACE validation, etc. (see e.g., Proteomic Validation: Brennan, S. O., et al., Albumin banks peninsula: a new termination variant characterized by electrospray mass spectrometry, Biochem Biophys Acta. 1999 Aug. 17; 1433(1-2):321-6; Ferranti P, et al., Differential splicing of pre-messenger RNA produces multiple forms of mature caprine alpha(s1)-casein, Eur J. Biochem. 1997 Oct. 1; 249(1):1-7. For PCR-based Validation: Wellmann S, et al., Specific reverse transcription-PCR quantification of vascular endothelial growth factor (VEGF) splice variants by LightCycler technology, Clin Chem. 2001 April; 47(4):654-60; Jia, H. P., et al., Discovery of new human beta-defensins using a genomics-based approach, Gene. 2001 Jan. 24; 263(1-2):211-8. For PCR-based and 5′ RACE Validation: Brigle, K. E., et al., Organization of the murine reduced folate carrier gene and identification of variant splice forms, Biochem Biophys Acta. 1997 Aug. 7; 1353(2): 191-8). 
     It is known in the art that genomic regions are modulated in cancers. When the genomic region to which a gene maps is modulated in a particular cancer, the alternative transcripts or splice variants of the gene are modulated as well. Disclosed herein is that 158P3D2 has a particular expression profile related to cancer. Alternative transcripts and splice variants of 158P3D2 may also be involved in cancers in the same or different tissues, thus serving as tumor-associated markers/antigens. 
     Using the full-length gene and EST sequences, six transcript variants were identified, designated as 158P3D2 v.2, v.14 through v.18. The boundaries of the exon in the original transcript, 158P3D2 v.1 were shown in Table LI. Exon compositions of the variants are shown in  FIG. 10 . Each different combination of exons in spatial order, e.g. exon 1 of v.2 and exons 3, 4, 5 and 6 of v.1, is a potential splice variant. 
     Tables LII(a)-(f) through LV(a)-(f) are set forth on a variant-by-variant bases. Tables LII(a)-(f) show nucleotide sequence of the transcript variants. Tables LIII(a)-(f) show the alignment of the respective transcript variant with nucleic acid sequence of 158P3D2 v.1. Tables LIV(a)-(f) lay out amino acid translation of the transcript variants for the identified reading frame orientation. Tables LV(a)-(f) displays alignments of the amino acid sequence encoded by the splice variant with that of 158P3D2 v.1. 
     Example 6 
     Single Nucleotide Polymorphisms of 158P3D2 
     A Single Nucleotide Polymorphism (SNP) is a single base pair variation in a nucleotide sequence at a specific location. At any given point of the genome, there are four possible nucleotide base pairs: A/T, C/G, G/C and T/A. Genotype refers to the specific base pair sequence of one or more locations in the genome of an individual. Haplotype refers to the base pair sequence of more than one location on the same DNA molecule (or the same chromosome in higher organisms), often in the context of one gene or in the context of several tightly linked genes. SNP that occurs on a cDNA is called cSNP. This cSNP may change amino acids of the protein encoded by the gene and thus change the functions of the protein. Some SNP cause inherited diseases; others contribute to quantitative variations in phenotype and reactions to environmental factors including diet and drugs among individuals. Therefore, SNP and/or combinations of alleles (called haplotypes) have many applications, including diagnosis of inherited diseases, determination of drug reactions and dosage, identification of genes responsible for diseases, and analysis of the genetic relationship between individuals (P. Nowotny, J. M. Kwon and A. M. Goate, “SNP analysis to dissect human traits,” Curr. Opin. Neurobiol. 2001 October; 11(5):637-641; M. Pirmohamed and B. K. Park, “Genetic susceptibility to adverse drug reactions,” Trends Pharmacol. Sci. 2001 June; 22(6):298-305; J. H. Riley, C. J. Allan, E. Lai and A. Roses, “The use of single nucleotide polymorphisms in the isolation of common disease genes,” Pharmacogenomics. 2000 February; 1(1):39-47; R. Judson, J. C. Stephens and A. Windemuth, “The predictive power of haplotypes in clinical response,” Pharmacogenomics. 2000 February; 1(1):15-26). 
     SNP are identified by a variety of art-accepted methods (P. Bean, “The promising voyage of SNP target discovery,” Am. Clin. Lab. 2001 October-November; 20(9):18-20; K. M. Weiss, “In search of human variation,” Genome Res. 1998 July; 8(7):691-697; M. M. She, “Enabling large-scale pharmacogenetic studies by high-throughput mutation detection and genotyping technologies,” Clin. Chem. 2001 February; 47(2):164-172). For example, SNP can be identified by sequencing DNA fragments that show polymorphism by gel-based methods such as restriction fragment length polymorphism (RFLP) and denaturing gradient gel electrophoresis (DGGE). They can also be discovered by direct sequencing of DNA samples pooled from different individuals or by comparing sequences from different DNA samples. With the rapid accumulation of sequence data in public and private databases, one can discover SNP by comparing sequences using computer programs (Z. Gu, L. Hillier and P. Y. Kwok, “Single nucleotide polymorphism hunting in cyberspace,” Hum. Mutat. 1998; 12(4):221-225). SNP can be verified and genotype or haplotype of an individual can be determined by a variety of methods including direct sequencing and high throughput microarrays (P. Y. Kwok, “Methods for genotyping single nucleotide polymorphisms,” Annu. Rev. Genomics Hum. Genet. 2001; 2:235-258; M. Kokoris, K. Dix, K. Moynihan, J. Mathis, B. Erwin, P. Grass, B. Hines and A. Duesterhoeft, “High-throughput SNP genotyping with the Masscode system,” Mol. Diagn. 2000 December; 5(4):329-340). 
     Using the methods described above, twelve SNP were identified in the original transcript, 158P3D2 v.1, at positions 1155 (T/C), 1152 (G/A), 960 (G/T) and 1236 (G/-), 519 (A/G), 440 (T/A), 971 (T/C), 150 (C/G), 1022 (C/A), 1148 (G/A), 1691 (G/T) and 1692 (A/G). The transcripts or proteins with alternative allele were designated as variant 158P3D2 v.3 through v.13, respectively.  FIG. 12  shows the schematic alignment of the SNP variants.  FIG. 11  shows the schematic alignment of protein variants, corresponding to nucleotide variants. Nucleotide variants that code for the same amino acid sequence as v.1 are not shown in  FIG. 11 . These alleles of the SNP, though shown separately here, can occur in different combinations (haplotypes) and in any one of the transcript variants (such as 158P3D2 v.17) that contains the site of the SNP. 
     Example 7 
     Production of Recombinant 158P3D2 in Prokaryotic Systems 
     To express recombinant 158P3D2 and 158P3D2 variants in prokaryotic cells, the full or partial length 158P3D2 and 158P3D2 variant cDNA sequences are cloned into any one of a variety of expression vectors known in the art. One or more of the following regions of 158P3D2 variants are expressed: the full length sequence presented in  FIGS. 2 and 3 , or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2, variants, or analogs thereof. 
     A. In Vitro Transcription and Translation Constructs 
     pCRII: 
     To generate 158P3D2 sense and anti-sense RNA probes for RNA in situ investigations, pCRII constructs (Invitrogen, Carlsbad Calif.) are generated encoding either all or fragments of the 158P3D2 cDNA. The pCRII vector has Sp6 and T7 promoters flanking the insert to drive the transcription of 158P3D2 RNA for use as probes in RNA in situ hybridization experiments. These probes are used to analyze the cell and tissue expression of 158P3D2 at the RNA level. Transcribed 158P3D2 RNA representing the cDNA amino acid coding region of the 158P3D2 gene is used during in vitro translation systems such as the TnT™ Coupled Reticulolysate System (Promega, Corp., Madison, Wis.) to synthesize 158P3D2 protein. 
     B. Bacterial Constructs 
     pGEX Constructs: 
     To generate recombinant 158P3D2 proteins in bacteria that are fused to the Glutathione S-transferase (GST) protein, all or parts of the 158P3D2 cDNA protein coding sequence are cloned into the pGEX family of GST-fusion vectors (Amersham Pharmacia Biotech, Piscataway, N.J.). These constructs allow controlled expression of recombinant 158P3D2 protein sequences with GST fused at the amino-terminus and a six histidine epitope (6×His) at the carboxyl-terminus The GST and 6×His tags permit purification of the recombinant fusion protein from induced bacteria with the appropriate affinity matrix and allow recognition of the fusion protein with anti-GST and anti-His antibodies. The 6×His tag is generated by adding 6 histidine codons to the cloning primer at the 3′ end, e.g., of the open reading frame (ORF). A proteolytic cleavage site, such as the PreScission™ recognition site in pGEX-6P-1, may be employed such that it permits cleavage of the GST tag from 158P3D2-related protein. The ampicillin resistance gene and pBR322 origin permits selection and maintenance of the pGEX plasmids in  E. coli.    
     pMAL Constructs: 
     To generate, in bacteria, recombinant 158P3D2 proteins that are fused to maltose-binding protein (MBP), all or parts of the 158P3D2 cDNA protein coding sequence are fused to the MBP gene by cloning into the pMAL-c2X and pMAL-p2X vectors (New England Biolabs, Beverly, Mass.). These constructs allow controlled expression of recombinant 158P3D2 protein sequences with MBP fused at the amino-terminus and a 6×His epitope tag at the carboxyl-terminus. The MBP and 6×His tags permit purification of the recombinant protein from induced bacteria with the appropriate affinity matrix and allow recognition of the fusion protein with anti-MBP and anti-His antibodies. The 6×His epitope tag is generated by adding 6 histidine codons to the 3′ cloning primer. A Factor Xa recognition site permits cleavage of the pMAL tag from 158P3D2. The pMAL-c2X and pMAL-p2X vectors are optimized to express the recombinant protein in the cytoplasm or periplasm respectively. Periplasm expression enhances folding of proteins with disulfide bonds. 
     pET Constructs: 
     To express 158P3D2 in bacterial cells, all or parts of the 158P3D2 cDNA protein coding sequence are cloned into the pET family of vectors (Novagen, Madison, Wis.). These vectors allow tightly controlled expression of recombinant 158P3D2 protein in bacteria with and without fusion to proteins that enhance solubility, such as NusA and thioredoxin (Trx), and epitope tags, such as 6×His and S-Tag™ that aid purification and detection of the recombinant protein. For example, constructs are made utilizing pET NusA fusion system 43.1 such that regions of the 158P3D2 protein are expressed as amino-terminal fusions to NusA. The cDNA encoding amino acids 155-290 and amino acids 260-328 of 158P3D2 each were cloned into the pET-21b vector. The recombinant proteins can be used to generate rabbit polyclonal antibodies. 
     C. Yeast Constructs: 
     pESC Constructs: 
     To express 158P3D2 in the yeast species  Saccharomyces cerevisiae  for generation of recombinant protein and functional studies, all or parts of the 158P3D2 cDNA protein coding sequence are cloned into the pESC family of vectors each of which contain 1 of 4 selectable markers, HIS3, TRP1, LEU2, and URA3 (Stratagene, La Jolla, Calif.). These vectors allow controlled expression from the same plasmid of up to 2 different genes or cloned sequences containing either Flag™ or Myc epitope tags in the same yeast cell. This system is useful to confirm protein-protein interactions of 158P3D2. In addition, expression in yeast yields similar post-translational modifications, such as glycosylations and phosphorylations, that are found when expressed in eukaryotic cells. 
     pESP Constructs: 
     To express 158P3D2 in the yeast species  Saccharomyces pombe , all or parts of the 158P3D2 cDNA protein coding sequence are cloned into the pESP family of vectors. These vectors allow controlled high level of expression of a 158P3D2 protein sequence that is fused at either the amino terminus or at the carboxyl terminus to GST which aids purification of the recombinant protein. A Flag™ epitope tag allows detection of the recombinant protein with anti-Flag™ antibody. 
     Example 8 
     Production of Recombinant 158P3D2 in Higher Eukaryotic Systems 
     A. Mammalian Constructs: 
     To express recombinant 158P3D2 in eukaryotic cells, the full or partial length 158P3D2 cDNA sequences, or variants thereof, can be cloned into any one of a variety of expression vectors known in the art. One or more of the following regions of 158P3D2 are expressed in these constructs, amino acids 1 to 328, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.1, v.3, v.4, v.10, v.12 and v.13; amino acids 1 to 236, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.2A; amino acids 1 to 181, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.2B or v.5B; amino acids 1 to 178, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.5A; amino acids 1 to 2036, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.17; amino acids 1 to 1990, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.16; amino acids 1 to 1145, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.15; amino acids 1 to 1393, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.14; amino acids 1 to 610, or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 158P3D2 v.18; or any 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more contiguous amino acids from 282P1G3 variants, or analogs thereof. 
     The constructs can be transfected into any one of a wide variety of mammalian cells such as 293T cells. Transfected 293T cell lysates can be probed with the anti-158P3D2 polyclonal serum, described herein. 
     pcDNA4/HisMax Constructs: 
     To express 158P3D2 in mammalian cells, a 158P3D2 ORF, or portions thereof, of 158P3D2 are cloned into pcDNA4/HisMax Version A (Invitrogen, Carlsbad, Calif.). Protein expression is driven from the cytomegalovirus (CMV) promoter and the SP16 translational enhancer. The recombinant protein has Xpress™ and six histidine (6×His) epitopes fused to the amino-terminus. The pcDNA4/HisMax vector also contains the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability along with the SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen. The Zeocin resistance gene allows for selection of mammalian cells expressing the protein and the ampicillin resistance gene and ColE1 origin permits selection and maintenance of the plasmid in  E. coli.    
     pcDNA3.1/MycHis Constructs: 
     To express 158P3D2 in mammalian cells, a 158P3D2 ORF, or portions thereof, of 158P3D2 with a consensus Kozak translation initiation site was cloned into pcDNA3.1/MycHis Version A (Invitrogen, Carlsbad, Calif.). Protein expression is driven from the cytomegalovirus (CMV) promoter. The recombinant proteins have the myc epitope and 6×His epitope fused to the carboxyl-terminus. The pcDNA3.1/MycHis vector also contains the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability, along with the SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen. The Neomycin resistance gene can be used, as it allows for selection of mammalian cells expressing the protein and the ampicillin resistance gene and ColE1 origin permits selection and maintenance of the plasmid in  E. coli .  FIG. 19  shows expression of 158P3D2.pcDNA3.1/mychis in transiently transfected 293T cells. 
     pcDNA3.1/CT-GFP-TOPO Construct: 
     To express 158P3D2 in mammalian cells and to allow detection of the recombinant proteins using fluorescence, a 158P3D2 ORF, or portions thereof, with a consensus Kozak translation initiation site are cloned into pcDNA3.1/CT-GFP-TOPO (Invitrogen, CA). Protein expression is driven from the cytomegalovirus (CMV) promoter. The recombinant proteins have the Green Fluorescent Protein (GFP) fused to the carboxyl-terminus facilitating non-invasive, in vivo detection and cell biology studies. The pcDNA3.1CT-GFP-TOPO vector also contains the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability along with the SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen. The Neomycin resistance gene allows for selection of mammalian cells that express the protein, and the ampicillin resistance gene and ColE1 origin permits selection and maintenance of the plasmid in  E. coli . Additional constructs with an amino-terminal GFP fusion are made in pcDNA3.1/NT-GFP-TOPO spanning the entire length of a 158P3D2 protein. 
     PAPtag: 
     A 158P3D2 ORF, or portions thereof, is cloned into pAPtag-5 (GenHunter Corp. Nashville, Tenn.). This construct generates an alkaline phosphatase fusion at the carboxyl-terminus of a 158P3D2 protein while fusing the IgGκ signal sequence to the amino-terminus. Constructs are also generated in which alkaline phosphatase with an amino-terminal IgGκ signal sequence is fused to the amino-terminus of a 158P3D2 protein. The resulting recombinant 158P3D2 proteins are optimized for secretion into the media of transfected mammalian cells and can be used to identify proteins such as ligands or receptors that interact with 158P3D2 proteins. Protein expression is driven from the CMV promoter and the recombinant proteins also contain myc and 6×His epitopes fused at the carboxyl-terminus that facilitates detection and purification. The Zeocin resistance gene present in the vector allows for selection of mammalian cells expressing the recombinant protein and the ampicillin resistance gene permits selection of the plasmid in  E. coli.    
     pTag5: 
     A 158P3D2 ORF, or portions thereof, is cloned into pTag-5. This vector is similar to pAPtag but without the alkaline phosphatase fusion. This construct generates 158P3D2 protein with an amino-terminal IgGκ signal sequence and myc and 6×His epitope tags at the carboxyl-terminus that facilitate detection and affinity purification. The resulting recombinant 158P3D2 protein is optimized for secretion into the media of transfected mammalian cells, and is used as immunogen or ligand to identify proteins such as ligands or receptors that interact with the 158P3D2 proteins. Protein expression is driven from the CMV promoter. The Zeocin resistance gene present in the vector allows for selection of mammalian cells expressing the protein, and the ampicillin resistance gene permits selection of the plasmid in  E. coli.    
     PsecFc: 
     A 158P3D2 ORF, or portions thereof, is also cloned into psecFc. The psecFc vector was assembled by cloning the human immunoglobulin G1 (IgG) Fc (hinge, CH2, CH3 regions) into pSecTag2 (Invitrogen, California). This construct generates an IgG1 Fc fusion at the carboxyl-terminus of the 158P3D2 proteins, while fusing the IgGK signal sequence to N-terminus. 158P3D2 fusions utilizing the murine IgG1 Fc region are also used. The resulting recombinant 158P3D2 proteins are optimized for secretion into the media of transfected mammalian cells, and can be used as immunogens or to identify proteins such as ligands or receptors that interact with 158P3D2 protein. Protein expression is driven from the CMV promoter. The hygromycin resistance gene present in the vector allows for selection of mammalian cells that express the recombinant protein, and the ampicillin resistance gene permits selection of the plasmid in  E. coli.    
     pSRα Constructs: 
     To generate mammalian cell lines that express 158P3D2 constitutively, 158P3D2 ORF, or portions thereof, of 158P3D2 were cloned into pSRα constructs. Amphotropic and ecotropic retroviruses were generated by transfection of pSRα constructs into the 293T-10A1 packaging line or co-transfection of pSRα and a helper plasmid (containing deleted packaging sequences) into the 293 cells, respectively. The retrovirus is used to infect a variety of mammalian cell lines, resulting in the integration of the cloned gene, 158P3D2, into the host cell-lines. Protein expression is driven from a long terminal repeat (LTR). The Neomycin resistance gene present in the vector allows for selection of mammalian cells that express the protein, and the ampicillin resistance gene and ColE1 origin permit selection and maintenance of the plasmid in  E. coli . The retroviral vectors can thereafter be used for infection and generation of various cell lines using, for example, PC3, NIH 3T3, TsuPr1, 293 or rat-1 cells. 
     Additional pSRα constructs are made that fuse an epitope tag such as the FLAG tag to the carboxyl-terminus of 158P3D2 sequences to allow detection using anti-Flag antibodies. For example, the FLAG sequence 5′ gat tac aag gat gac gac gat aag 3′ (SEQ ID NO:103) is added to cloning primer at the 3′ end of the ORF. Additional pSRα constructs are made to produce both amino-terminal and carboxyl-terminal GFP and myc/6×His fusion proteins of the full-length 158P3D2 proteins. 
     Additional Viral Vectors: 
     Additional constructs are made for viral-mediated delivery and expression of 158P3D2. High virus titer leading to high level expression of 158P3D2 is achieved in viral delivery systems such as adenoviral vectors and herpes amplicon vectors. A 158P3D2 coding sequences or fragments thereof are amplified by PCR and subcloned into the AdEasy shuttle vector (Stratagene). Recombination and virus packaging are performed according to the manufacturer&#39;s instructions to generate adenoviral vectors. Alternatively, 158P3D2 coding sequences or fragments thereof are cloned into the HSV-1 vector (Imgenex) to generate herpes viral vectors. The viral vectors are thereafter used for infection of various cell lines such as PC3, NIH 3T3, 293 or rat-1 cells. 
     Regulated Expression Systems: 
     To control expression of 158P3D2 in mammalian cells, coding sequences of 158P3D2, or portions thereof, are cloned into regulated mammalian expression systems such as the T-Rex System (Invitrogen), the GeneSwitch System (Invitrogen) and the tightly-regulated Ecdysone System (Sratagene). These systems allow the study of the temporal and concentration dependent effects of recombinant 158P3D2. These vectors are thereafter used to control expression of 158P3D2 in various cell lines such as PC3, NIH 3T3, 293 or rat-1 cells. 
     B. Baculovirus Expression Systems 
     To generate recombinant 158P3D2 proteins in a baculovirus expression system, 158P3D2 ORF, or portions thereof, are cloned into the baculovirus transfer vector pBlueBac 4.5 (Invitrogen), which provides a His-tag at the N-terminus. Specifically, pBlueBac-158P3D2 is co-transfected with helper plasmid pBac-N-Blue (Invitrogen) into SF9 ( Spodoptera frugiperda ) insect cells to generate recombinant baculovirus (see Invitrogen instruction manual for details). Baculovirus is then collected from cell supernatant and purified by plaque assay. 
     Recombinant 158P3D2 protein is then generated by infection of HighFive insect cells (Invitrogen) with purified baculovirus. Recombinant 158P3D2 protein can be detected using anti-158P3D2 or anti-His-tag antibody. 158P3D2 protein can be purified and used in various cell-based assays or as immunogen to generate polyclonal and monoclonal antibodies specific for 158P3D2 which are used for diagnostic and therapeutic purposes. 
     Example 9 
     Antigenicity Profiles and Secondary Structure 
       FIG. 5A-I ,  FIG. 6A-I ,  FIG. 7A-I ,  FIG. 8A-I , and  FIG. 9A-I  depict graphically five amino acid profiles of 158P3D2 variants 1, 2a, 2b, 5a, 14, 15, 16, 17, 18, (A) through (I) respectively, each assessment available by accessing the ProtScale website on the ExPasy molecular biology server. 
     These profiles:  FIG. 5 , Hydrophilicity, (Hopp T. P., Woods K. R., 1981. Proc. Natl. Acad. Sci. U.S.A. 78:3824-3828);  FIG. 6 , Hydropathicity, (Kyte J., Doolittle R. F., 1982. J. Mol. Biol. 157:105-132);  FIG. 7 , Percentage Accessible Residues (Janin J., 1979 Nature 277:491-492);  FIG. 8 , Average Flexibility, (Bhaskaran R., and Ponnuswamy P. K., 1988. Int. J. Pept. Protein Res. 32:242-255);  FIG. 9 , Beta-turn (Deleage, G., Roux B. 1987 Protein Engineering 1:289-294); and optionally others available in the art, such as on the ProtScale website, were used to identify antigenic regions of each of the 158P3D2 variant proteins. Each of the above amino acid profiles of 158P3D2 variants were generated using the following ProtScale parameters for analysis: 1) A window size of 9; 2) 100% weight of the window edges compared to the window center; and, 3) amino acid profile values normalized to lie between 0 and 1. 
     Hydrophilicity ( FIG. 5 ), Hydropathicity ( FIG. 6 ) and Percentage Accessible Residues ( FIG. 7 ) profiles were used to determine stretches of hydrophilic amino acids (i.e., values greater than 0.5 on the Hydrophilicity and Percentage Accessible Residues profile, and values less than 0.5 on the Hydropathicity profile). Such regions are likely to be exposed to the aqueous environment, be present on the surface of the protein, and thus available for immune recognition, such as by antibodies. 
     Average Flexibility ( FIG. 8 ) and Beta-turn ( FIG. 9 ) profiles determine stretches of amino acids (i.e., values greater than 0.5 on the Beta-turn profile and the Average Flexibility profile) that are not constrained in secondary structures such as beta sheets and alpha helices. Such regions are also more likely to be exposed on the protein and thus accessible to immune recognition, such as by antibodies. 
     Antigenic sequences of the 158P3D2 variant proteins indicated, e.g., by the profiles set forth in  FIG. 5 ,  FIG. 6 ,  FIG. 7 ,  FIG. 8 , and/or  FIG. 9  are used to prepare immunogens, either peptides or nucleic acids that encode them, to generate therapeutic and diagnostic anti-158P3D2 antibodies. The immunogen can be any 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 35, 40, 45, 50 or more than 50 contiguous amino acids, or the corresponding nucleic acids that encode them, from the 158P3D2 protein variants listed in  FIGS. 2 and 3 . In particular, peptide immunogens of the invention can comprise, a peptide region of at least 5 amino acids of  FIGS. 2 and 3  in any whole number increment that includes an amino acid position having a value greater than 0.5 in the Hydrophilicity profiles of  FIG. 5 ; a peptide region of at least 5 amino acids of  FIGS. 2 and 3  in any whole number increment that includes an amino acid position having a value less than 0.5 in the Hydropathicity profile of  FIG. 6 ; a peptide region of at least 5 amino acids of  FIGS. 2 and 3  in any whole number increment that includes an amino acid position having a value greater than 0.5 in the Percent Accessible Residues profiles of  FIG. 7 ; a peptide region of at least 5 amino acids of  FIGS. 2 and 3  in any whole number increment that includes an amino acid position having a value greater than 0.5 in the Average Flexibility profiles on  FIG. 8 ; and, a peptide region of at least 5 amino acids of  FIGS. 2 and 3  in any whole number increment that includes an amino acid position having a value greater than 0.5 in the Beta-turn profile of  FIG. 9 . Peptide immunogens of the invention can also comprise nucleic acids that encode any of the forgoing. 
     All immunogens of the invention, peptide or nucleic acid, can be embodied in human unit dose form, or comprised by a composition that includes a pharmaceutical excipient compatible with human physiology. 
     The secondary structures of 158P3D2 protein variants 1, 2a, 2b, 5a, 14, 15, 16, 17, and 18, namely the predicted presence and location of alpha helices, extended strands, and random coils, are predicted from their primary amino acid sequences using the HNN—Hierarchical Neural Network method (NPS@: Network Protein Sequence Analysis TIBS 2000 March Vol. 25, No 3 [291]:147-150 Combet C., Blanchet C., Geourjon C. and Deleage G., accessed from the ExPasy molecular biology server. The analysis indicates that 158P3D2 variant 1 is composed of 32.93% alpha helix, 18.29% extended strand, and 48.78% random coil ( FIG. 13A ). 158P3D2 variant 2a is composed of 25.58% alpha helix, 18.22% extended strand, and 55.93% random coil ( FIG. 13B ). 158P3D2 variant 2b is composed of 44.75% alpha helix, 11.60% extended strand, and 43.65% random coil ( FIG. 13C ). 158P3D2 variant 5a is composed of 9.55% alpha helix, 26.40% extended strand, and 64.04% random coil ( FIG. 13D ). 158P3D2 variant 14 is composed of 33.88% alpha helix, 13.42% extended strand, and 52.69% random coil ( FIG. 13E ). 158P3D2 variant 15 is composed of 33.28% alpha helix, 15.11% extended strand, and 51.62% random coil ( FIG. 13F ). 158P3D2 variant 16 is composed of 32.76% alpha helix, 14.47% extended strand, and 52.76% random coil ( FIG. 13G ). 158P3D2 variant 17 is composed of 32.86% alpha helix, 14.69% extended strand, and 52.46% random coil ( FIG. 13H ). 158P3D2 variant 18 is composed of 27.21% alpha helix, 14.75% extended strand, and 58.03% random coil ( FIG. 13I ). 
     Analysis for the potential presence of transmembrane domains in the 158P3D2 variant proteins 1, 2a, 2b, 5a, 14, 15, 16, 17, and 18, was carried out using a variety of transmembrane prediction algorithms accessed from the ExPasy molecular biology server. Shown graphically in  FIGS. 13L ,  13 N,  13 P,  13 R,  13 T,  13 V,  13 X,  13 Z are the results of analysis of variants 1, 2a, 2b, 5a, 14, 15, 16, 17, and 18, respectively, using the TMpred program. Shown graphically in  FIGS. 13K ,  13 M,  13 O,  13 Q,  13 S,  13 U,  13 W,  13 Y,  13 AA are the results of analysis of variants 1, 2a, 2b, 5a, 14, 15, 16, 17, and 18, respectively using the TMHMM program. Both programs predict the presence of 1 transmembrane domain in variant 1, Both programs predict that variants 2a, 2b, 5a, and 18 lack transmembrane domains and are soluble proteins. The TMpred program predicts that variants 14, 15, 16, and 17 have 2 transmembrane domains of which the more carboxy-terminal transmembrane has a higher probability of existence. The TMHMM program predicts that variants 14 and 15 do not encode transmembrane domains and variants 16 and 17 contain 1 transmembrane domain. Analyses of the variants using other structural prediction programs are summarized in Table VI and Table L. 
     Example 10 
     Generation of 158P3D2 Polyclonal Antibodies 
     Polyclonal antibodies can be raised in a mammal, for example, by one or more injections of an immunizing agent and, if desired, an adjuvant. Typically, the immunizing agent and/or adjuvant will be injected in the mammal by multiple subcutaneous or intraperitoneal injections. In addition to immunizing with a full length 158P3D2 protein variant, computer algorithms are employed in design of immunogens that, based on amino acid sequence analysis contain characteristics of being antigenic and available for recognition by the immune system of the immunized host (see the Example entitled “Antigenicity Profiles and Secondary Structure”). Such regions would be predicted to be hydrophilic, flexible, in beta-turn conformations, and be exposed on the surface of the protein (see, e.g.,  FIG. 5 ,  FIG. 6 ,  FIG. 7 ,  FIG. 8 , or  FIG. 9  for amino acid profiles that indicate such regions of 158P3D2 protein variant 1 or other protein variants). 
     For example, recombinant bacterial fusion proteins or peptides containing hydrophilic, flexible, beta-turn regions of 158P3D2 protein variants are used as antigens to generate polyclonal antibodies in New Zealand White rabbits or monoclonal antibodies as described in Example 11 (“Generation of Monoclonal Antibodies”). For example, in 158P3D2 variant 1, such regions include, but are not limited to, amino acids 1-25, amino acids 37-54, amino acids 60-73, amino acids 187-225, and amino acids 235-271. An extracellular epitope peptide encoding amino acids 315 to 328 is also used to generate antibodies that bind to the extracellular region of 158P3D2 protein. It is useful to conjugate the immunizing agent to a protein known to be immunogenic in the mammal being immunized. Examples of such immunogenic proteins include, but are not limited to, keyhole limpet hemocyanin (KLH), serum albumin, bovine thyroglobulin, and soybean trypsin inhibitor. In one embodiment, a peptide encoding amino acids 315-328 of 158P3D2 variant 1 was conjugated to KLH and used to immunize a rabbit. Alternatively the immunizing agent may include all or portions of the 158P3D2 variant proteins, analogs or fusion proteins thereof. For example, the 158P3D2 variant 1 amino acid sequence can be fused using recombinant DNA techniques to any one of a variety of fusion protein partners that are well known in the art, such as glutathione-S-transferase (GST) and HIS tagged fusion proteins. 
     In one embodiment, amino acids 155-290 of 158P3D2 variant 1 were fused to His using recombinant techniques and the pET21b expression vector. In another embodiment, amino acids 260-328 were cloned into the pET21b expression vector. The proteins are then expressed, purified, and used to immunize rabbits. Such fusion proteins are purified from induced bacteria using the appropriate affinity matrix. 
     Other recombinant bacterial fusion proteins that may be employed include maltose binding protein, LacZ, thioredoxin, NusA, or an immunoglobulin constant region (see the section entitled “Production of 158P3D2 in Prokaryotic Systems” and Current Protocols In Molecular Biology, Volume 2, Unit 16, Frederick M. Ausubul et al. eds., 1995; Linsley, P. S., Brady, W., Urnes, M., Grosmaire, L., Damle, N., and Ledbetter, L. (1991) J. Exp. Med. 174, 561-566). 
     In addition to bacterial derived fusion proteins, mammalian expressed protein antigens are also used. These antigens are expressed from mammalian expression vectors such as the TagS and Fc-fusion vectors (see the section entitled “Production of Recombinant 158P3D2 in Eukaryotic Systems”), and retain post-translational modifications such as glycosylations found in native protein. In one embodiment, amino acids 1-236 of 158P3D2 variant 2a is cloned into the TagS mammalian secretion vector, and expressed in 293T cells. The recombinant protein is purified by metal chelate chromatography from tissue culture supernatants of 293T cells stably expressing the recombinant vector. The purified TagS 158P3D2 protein is then used as immunogen. 
     During the immunization protocol, it is useful to mix or emulsify the antigen in adjuvants that enhance the immune response of the host animal. Examples of adjuvants include, but are not limited to, complete Freund&#39;s adjuvant (CFA) and MPL-TDM adjuvant (monophosphoryl Lipid A, synthetic trehalose dicorynomycolate). 
     In a typical protocol, rabbits are initially immunized subcutaneously with up to 200 μg, typically 100-200 μg, of fusion protein or peptide conjugated to KLH mixed in complete Freund&#39;s adjuvant (CFA). Rabbits are then injected subcutaneously every two weeks with up to 200 μg, typically 100-200 μg, of the immunogen in incomplete Freund&#39;s adjuvant (IFA). Test bleeds are taken approximately 7-10 days following each immunization and used to monitor the titer of the antiserum by ELISA. 
     To test reactivity and specificity of immune serum, such a rabbit serum derived from immunization with the His-fusion of 158P3D2 variant 1 protein, the full-length 158P3D2 variant 1 cDNA was cloned into pcDNA 3.1 myc-his expression vector (Invitrogen, see the Example entitled “Production of Recombinant 158P3D2 in Eukaryotic Systems”). After transfection of the constructs into 293T cells, cell lysates are probed with the anti-158P3D2 serum and with anti-His antibody (Santa Cruz Biotechnologies, Santa Cruz, Calif.) to determine specific reactivity to denatured 158P3D2 protein using the Western blot technique. In addition, the immune serum is tested by fluorescence microscopy, flow cytometry and immunoprecipitation against 293T and other recombinant 158P3D2-expressing cells to determine specific recognition of native protein. Western blot, immunoprecipitation, fluorescent microscopy, and flow cytometric techniques using cells that endogenously express 158P3D2 are also carried out to test reactivity and specificity. 
     Anti-serum from rabbits immunized with 158P3D2 variant fusion proteins, such as GST and MBP fusion proteins, are purified by depletion of antibodies reactive to the fusion partner sequence by passage over an affinity column containing the fusion partner either alone or in the context of an irrelevant fusion protein. For example, antiserum derived from a GST-158P3D2 variant 1 fusion protein is first purified by passage over a column of GST protein covalently coupled to AffiGel matrix (BioRad, Hercules, Calif.). The antiserum is then affinity purified by passage over a column composed of a MBP-158P3D2 fusion protein covalently coupled to Affigel matrix. The serum is then further purified by protein G affinity chromatography to isolate the IgG fraction. Sera from other His-tagged antigens and peptide immunized rabbits as well as fusion partner depleted sera are affinity purified by passage over a column matrix composed of the original protein immunogen or free peptide. 
     Example 11 
     Generation of 158P3D2 Monoclonal Antibodies (mAbs) 
     In one embodiment, therapeutic mAbs to 158P3D2 variants comprise those that react with epitopes specific for each variant protein or specific to sequences in common between the variants that would disrupt or modulate the biological function of the 158P3D2 variants, for example those that would disrupt the interaction with ligands and binding partners. Immunogens for generation of such mAbs include those designed to encode or contain the entire 158P3D2 protein variant sequence, regions predicted to contain functional motifs, and regions of the 158P3D2 protein variants predicted to be antigenic from computer analysis of the amino acid sequence (see, e.g.,  FIG. 5 ,  FIG. 6 ,  FIG. 7 ,  FIG. 8 , or  FIG. 9 , and the Example entitled “Antigenicity Profiles and Secondary Structure”) Immunogens include peptides, recombinant bacterial proteins, and mammalian expressed Tag 5 proteins and human and murine IgG FC fusion proteins. In addition, cells engineered to express high levels of a respective 158P3D2 variant, such as Rat1-158P3D2 variant 1 or 300.19-158P3D2 variant 1 murine Pre-B cells, were used to immunize mice. 
     To generate mAbs to a 158P3D2 variant, mice are first immunized intraperitoneally (IP) with, typically, 10-50 μg of protein immunogen or 10 7  158P3D2-expressing cells mixed in complete Freund&#39;s adjuvant. Mice are then subsequently immunized IP every 2-4 weeks with, typically, 10-50 μg of protein immunogen or 10 7  cells mixed in incomplete Freund&#39;s adjuvant. Alternatively, MPL-TDM adjuvant is used in immunizations. In addition to the above protein and cell-based immunization strategies, a DNA-based immunization protocol is employed in which a mammalian expression vector encoding a 158P3D2 variant sequence is used to immunize mice by direct injection of the plasmid DNA. For example, amino acids 1-400 of 158P3D2 of variant 15 is cloned into the TagS mammalian secretion vector and the recombinant vector will then be used as immunogen. In another example the same amino acids are cloned into an Fc-fusion secretion vector in which the 158P3D2 variant 15 sequence is fused at the amino-terminus to an IgK leader sequence and at the carboxyl-terminus to the coding sequence of the human or murine IgG Fc region. This recombinant vector is then used as immunogen. The plasmid immunization protocols are used in combination with purified proteins expressed from the same vector and with cells expressing the respective 158P3D2 variant. 
     During the immunization protocol, test bleeds are taken 7-10 days following an injection to monitor titer and specificity of the immune response. Once appropriate reactivity and specificity is obtained as determined by ELISA, Western blotting, immunoprecipitation, fluorescence microscopy, and flow cytometric analyses, fusion and hybridoma generation is then carried out with established procedures well known in the art (see, e.g., Harlow and Lane, 1988). 
     In one embodiment for generating 158P3D2 monoclonal antibodies, a peptide encoding amino acids 315-328 of 158P3D2 variant 1 was coupled to KLH and use to immunize mice. Balb C mice were immunized with 10 μg of the KLH-peptide mixed in adjuvant. Mice were subsequently immunized over several weeks with the KLH-peptide. ELISA using the peptide coupled to a different carrier, ovalbumin, determined the titer of serum from the immunized mice (See  FIG. 29 ). Reactivity and specificity of the serum to full length 158P3D2 variant 1 protein was monitored by flow cytometry and Western blotting using recombinant 158P3D2 variant 1-expressing cells and cells endogenously expressing 158P3D2 variant 1 protein. Mice showing the strongest reactivity were rested and given a final injection of antigen and sacrificed for fusion. The lymph nodes of the sacrificed mice were harvested and fused to SPO/2 myeloma cells using standard procedures (Harlow and Lane, 1988). Supernatants from HAT selected growth wells were analyzed by flow cytometry to identify specific-158P3D2 surface binding MAbs. Supernatants were also screened by ELISA, Western blot, immunoprecipitation, and fluorescent microscopy to identify 158P3D2 specific antibody-producing clones. 
     In other embodiments, 158P3D2 variant specific MAbs are generated by employing immunogens that encode amino acid sequences unique to each variant or created by unique junctions from alternative splicing of exons. For example, a peptide encoding amino acids 1018-1035 of 158P3D2 variant 15 is coupled to KLH and used to immunize mice. In another example, amino acids 1375-1393 of 158P3D2 variant 14 is coupled to KLH and used to immunize mice. Hybridomas resulting from fusion of the B-cells from the mice are screened on cells expressing the respective 158P3D2 variant protein from which the antigen was derived and cross-screened on cells expressing the other variant proteins to identify variant specific MAbs and MAbs that may recognize more than 1 variant. 
     The binding affinity of 158P3D2 variant specific monoclonal antibodies was determined using standard technologies. Affinity measurements quantify the strength of antibody to epitope binding and are used to help define which 158P3D2 variant monoclonal antibodies preferred for diagnostic or therapeutic use, as appreciated by one of skill in the art. The BIAcore system (Uppsala, Sweden) is a preferred method for determining binding affinity. The BIAcore system uses surface plasmon resonance (SPR, Welford K. 1991, Opt. Quant. Elect. 23:1; Morton and Myszka, 1998, Methods in Enzymology 295: 268) to monitor biomolecular interactions in real time. BIAcore analysis conveniently generates association rate constants, dissociation rate constants, equilibrium dissociation constants, and affinity constants. 
     In addition, equilibrium binding analysis of a dilution series of the MAb was also used to determine affinity defined by the dissociation constant (KD). The KD is determined by non-linear regression of the equilibrium binding data of the concentration series. The KD is defined as the concentration at which half-maximal binding of the MAb to the antigen is attained under equilibrium conditions. 
     Example 12 
     HLA Class I and Class II Binding Assays 
     HLA class I and class II binding assays using purified HLA molecules are performed in accordance with disclosed protocols (e.g., PCT publications WO 94/20127 and WO 94/03205; Sidney et al., Current Protocols in Immunology 18.3.1 (1998); Sidney, et al., J. Immunol. 154:247 (1995); Sette, et al., Mol. Immunol. 31:813 (1994)). Briefly, purified MHC molecules (5 to 500 nM) are incubated with various unlabeled peptide inhibitors and 1-10 nM 125I-radiolabeled probe peptides as described. Following incubation, MHC-peptide complexes are separated from free peptide by gel filtration and the fraction of peptide bound is determined. Typically, in preliminary experiments, each MHC preparation is titered in the presence of fixed amounts of radiolabeled peptides to determine the concentration of HLA molecules necessary to bind 10-20% of the total radioactivity. All subsequent inhibition and direct binding assays are performed using these HLA concentrations. 
     Since under these conditions [label]&lt;[HLA] and IC50≧[HLA], the measured IC50 values are reasonable approximations of the true KD values. Peptide inhibitors are typically tested at concentrations ranging from 120 μg/ml to 1.2 ng/ml, and are tested in two to four completely independent experiments. To allow comparison of the data obtained in different experiments, a relative binding figure is calculated for each peptide by dividing the IC50 of a positive control for inhibition by the IC50 for each tested peptide (typically unlabeled versions of the radiolabeled probe peptide). For database purposes, and inter-experiment comparisons, relative binding values are compiled. These values can subsequently be converted back into IC50 nM values by dividing the IC50 nM of the positive controls for inhibition by the relative binding of the peptide of interest. This method of data compilation is accurate and consistent for comparing peptides that have been tested on different days, or with different lots of purified MHC. 
     Binding assays as outlined above may be used to analyze HLA supermotif and/or HLA motif-bearing peptides (see Table IV). 
     Example 13 
     Identification of HLA Supermotif- and Motif-Bearing CTL Candidate Epitopes 
     HLA vaccine compositions of the invention can include multiple epitopes. The multiple epitopes can comprise multiple HLA supermotifs or motifs to achieve broad population coverage. This example illustrates the identification and confirmation of supermotif- and motif-bearing epitopes for the inclusion in such a vaccine composition. Calculation of population coverage is performed using the strategy described below. 
     Computer searches and algorithms for identification of supermotif and/or motif-bearing epitopes 
     The searches performed to identify the motif-bearing peptide sequences in the Example entitled “Antigenicity Profiles” and Tables VIII-XXI and XXII-XLIX employ the protein sequence data from the gene product of 158P3D2 set forth in  FIGS. 2 and 3 , the specific search peptides used to generate the tables are listed in Table VII. 
     Computer searches for epitopes bearing HLA Class I or Class II supermotifs or motifs are performed as follows. All translated 158P3D2 protein sequences are analyzed using a text string search software program to identify potential peptide sequences containing appropriate HLA binding motifs; such programs are readily produced in accordance with information in the art in view of known motif/supermotif disclosures. Furthermore, such calculations can be made mentally. 
     Identified A2-, A3-, and DR-supermotif sequences are scored using polynomial algorithms to predict their capacity to bind to specific HLA-Class I or Class II molecules. These polynomial algorithms account for the impact of different amino acids at different positions, and are essentially based on the premise that the overall affinity (or ΔG) of peptide-HLA molecule interactions can be approximated as a linear polynomial function of the type:
 
“Δ G”=a 1 i×a 2 i×a 3 i . . . ×ani  
 
     where aji is a coefficient which represents the effect of the presence of a given amino acid (j) at a given position (i) along the sequence of a peptide of n amino acids. The crucial assumption of this method is that the effects at each position are essentially independent of each other (i.e., independent binding of individual side-chains). When residue j occurs at position i in the peptide, it is assumed to contribute a constant amount ji to the free energy of binding of the peptide irrespective of the sequence of the rest of the peptide. 
     The method of derivation of specific algorithm coefficients has been described in Gulukota et al., J. Mol. Biol. 267:1258-126, 1997; (see also Sidney et al., Human Immunol. 45:79-93, 1996; and Southwood et al., J. Immunol. 160:3363-3373, 1998). Briefly, for all i positions, anchor and non-anchor alike, the geometric mean of the average relative binding (ARB) of all peptides carrying j is calculated relative to the remainder of the group, and used as the estimate of ji. For Class II peptides, if multiple alignments are possible, only the highest scoring alignment is utilized, following an iterative procedure. To calculate an algorithm score of a given peptide in a test set, the ARB values corresponding to the sequence of the peptide are multiplied. If this product exceeds a chosen threshold, the peptide is predicted to bind. Appropriate thresholds are chosen as a function of the degree of stringency of prediction desired. 
     Selection of HLA-A2 Supertype Cross-Reactive Peptides 
     Protein sequences from 158P3D2 are scanned utilizing motif identification software, to identify 8-, 9-10- and 11-mer sequences containing the HLA-A2-supermotif main anchor specificity. Typically, these sequences are then scored using the protocol described above and the peptides corresponding to the positive-scoring sequences are synthesized and tested for their capacity to bind purified HLA-A*0201 molecules in vitro (HLA-A*0201 is considered a prototype A2 supertype molecule). 
     These peptides are then tested for the capacity to bind to additional A2-supertype molecules (A*0202, A*0203, A*0206, and A*6802). Peptides that bind to at least three of the five A2-supertype alleles tested are typically deemed A2-supertype cross-reactive binders. Preferred peptides bind at an affinity equal to or less than 500 nM to three or more HLA-A2 supertype molecules. 
     Selection of HLA-A3 Supermotif-Bearing Epitopes 
     The 158P3D2 protein sequence(s) scanned above is also examined for the presence of peptides with the HLA-A3-supermotif primary anchors. Peptides corresponding to the HLA A3 supermotif-bearing sequences are then synthesized and tested for binding to HLA-A*0301 and HLA-A*1101 molecules, the molecules encoded by the two most prevalent A3-supertype alleles. The peptides that bind at least one of the two alleles with binding affinities of 500 nM, often 200 nM, are then tested for binding cross-reactivity to the other common A3-supertype alleles (e.g., A*3101, A*3301, and A*6801) to identify those that can bind at least three of the five HLA-A3-supertype molecules tested. 
     Selection of HLA-B7 Supermotif Bearing Epitopes 
     The 158P3D2 protein(s) scanned above is also analyzed for the presence of 8-, 9-10-, or 11-mer peptides with the HLA-B7-supermotif. Corresponding peptides are synthesized and tested for binding to HLA-B*0702, the molecule encoded by the most common B7-supertype allele (i.e., the prototype B7 supertype allele). Peptides binding B*0702 with IC50 of ≦500 nM are identified using standard methods. These peptides are then tested for binding to other common B7-supertype molecules (e.g., B*3501, B*5101, B*5301, and B*5401). Peptides capable of binding to three or more of the five B7-supertype alleles tested are thereby identified. 
     Selection of A1 and A24 Motif-Bearing Epitopes 
     To further increase population coverage, HLA-A1 and -A24 epitopes can also be incorporated into vaccine compositions. An analysis of the 158P3D2 protein can also be performed to identify HLA-A1- and A24-motif-containing sequences. 
     High affinity and/or cross-reactive binding epitopes that bear other motif and/or supermotifs are identified using analogous methodology. 
     Example 14 
     Confirmation of Immunogenicity 
     Cross-reactive candidate CTL A2-supermotif-bearing peptides that are identified as described herein are selected to confirm in vitro immunogenicity. Confirmation is performed using the following methodology: 
     Target Cell Lines for Cellular Screening: 
     The 0.221A2.1 cell line, produced by transferring the HLA-A2.1 gene into the HLA-A, -B, -C null mutant human B-lymphoblastoid cell line 721.221, is used as the peptide-loaded target to measure activity of HLA-A2.1-restricted CTL. This cell line is grown in RPMI-1640 medium supplemented with antibiotics, sodium pyruvate, nonessential amino acids and 10% (v/v) heat inactivated FCS. Cells that express an antigen of interest, or transfectants comprising the gene encoding the antigen of interest, can be used as target cells to confirm the ability of peptide-specific CTLs to recognize endogenous antigen. 
     Primary CTL Induction Cultures: 
     Generation of Dendritic Cells (DC): PBMCs are thawed in RPMI with 30 μg/ml DNAse, washed twice and resuspended in complete medium (RPMI-1640 plus 5% AB human serum, non-essential amino acids, sodium pyruvate, L-glutamine and penicillin/streptomycin). The monocytes are purified by plating 10×106 PBMC/well in a 6-well plate. After 2 hours at 37° C., the non-adherent cells are removed by gently shaking the plates and aspirating the supernatants. The wells are washed a total of three times with 3 ml RPMI to remove most of the non-adherent and loosely adherent cells. Three ml of complete medium containing 50 ng/ml of GM-CSF and 1,000 U/ml of IL-4 are then added to each well. TNFα is added to the DCs on day 6 at 75 ng/ml and the cells are used for CTL induction cultures on day 7. 
     Induction of CTL with DC and Peptide: CD8+ T-cells are isolated by positive selection with Dynal immunomagnetic beads (Dynabeads® M-450) and the Detacha-Bead® reagent. Typically about 200-250×106 PBMC are processed to obtain 24×106 CD8+ T-cells (enough for a 48-well plate culture). Briefly, the PBMCs are thawed in RPMI with 30 μg/ml DNAse, washed once with PBS containing 1% human AB serum and resuspended in PBS/1% AB serum at a concentration of 20×106 cells/ml. The magnetic beads are washed 3 times with PBS/AB serum, added to the cells (140 μl beads/20×106 cells) and incubated for 1 hour at 4° C. with continuous mixing. The beads and cells are washed 4× with PBS/AB serum to remove the nonadherent cells and resuspended at 100×106 cells/ml (based on the original cell number) in PBS/AB serum containing 100 μl/ml Detacha-Bead® reagent and 30 μg/ml DNAse. The mixture is incubated for 1 hour at room temperature with continuous mixing. The beads are washed again with PBS/AB/DNAse to collect the CD8+ T-cells. The DC are collected and centrifuged at 1300 rpm for 5-7 minutes, washed once with PBS with 1% BSA, counted and pulsed with 40 μg/ml of peptide at a cell concentration of 1-2×106/ml in the presence of 3 μg/ml β2-microglobulin for 4 hours at 20° C. The DC are then irradiated (4,200 rads), washed 1 time with medium and counted again. 
     Setting up induction cultures: 0.25 ml cytokine-generated DC (at 1×105 cells/ml) are co-cultured with 0.25 ml of CD8+ T-cells (at 2×106 cell/ml) in each well of a 48-well plate in the presence of 10 ng/ml of IL-7. Recombinant human IL-10 is added the next day at a final concentration of 10 ng/ml and rhuman IL-2 is added 48 hours later at 10 IU/ml. 
     Restimulation of the induction cultures with peptide-pulsed adherent cells: Seven and fourteen days after the primary induction, the cells are restimulated with peptide-pulsed adherent cells. The PBMCs are thawed and washed twice with RPMI and DNAse. The cells are resuspended at 5×106 cells/ml and irradiated at ˜4200 rads. The PBMCs are plated at 2×106 in 0.5 ml complete medium per well and incubated for 2 hours at 37° C. The plates are washed twice with RPMI by tapping the plate gently to remove the nonadherent cells and the adherent cells pulsed with 10 μg/ml of peptide in the presence of 3 μg/ml β2 microglobulin in 0.25 ml RPMI/5% AB per well for 2 hours at 37° C. Peptide solution from each well is aspirated and the wells are washed once with RPMI. Most of the media is aspirated from the induction cultures (CD8+ cells) and brought to 0.5 ml with fresh media. The cells are then transferred to the wells containing the peptide-pulsed adherent cells. Twenty four hours later recombinant human IL-10 is added at a final concentration of 10 ng/ml and recombinant human IL2 is added the next day and again 2-3 days later at 501 U/ml (Tsai et al., Critical Reviews in Immunology 18(1-2):65-75, 1998). Seven days later, the cultures are assayed for CTL activity in a 51Cr release assay. In some experiments the cultures are assayed for peptide-specific recognition in the in situ IFNγ ELISA at the time of the second restimulation followed by assay of endogenous recognition 7 days later. After expansion, activity is measured in both assays for a side-by-side comparison. 
     Measurement of CTL Lytic Activity by 51Cr Release. 
     Seven days after the second restimulation, cytotoxicity is determined in a standard (5 hr) 51Cr release assay by assaying individual wells at a single E:T. Peptide-pulsed targets are prepared by incubating the cells with 10 μg/ml peptide overnight at 37° C. 
     Adherent target cells are removed from culture flasks with trypsin-EDTA. Target cells are labeled with 200 μCi of 51Cr sodium chromate (Dupont, Wilmington, Del.) for 1 hour at 37° C. Labeled target cells are resuspended at 106 per ml and diluted 1:10 with K562 cells at a concentration of 3.3×106/ml (an NK-sensitive erythroblastoma cell line used to reduce non-specific lysis). Target cells (100 μl) and effectors (100 μl) are plated in 96 well round-bottom plates and incubated for 5 hours at 37° C. At that time, 100 μl of supernatant are collected from each well and percent lysis is determined according to the formula:
 
[(cpm of the test sample−cpm of the spontaneous 51Cr release sample)/(cpm of the maximal 51Cr release sample−cpm of the spontaneous 51Cr release sample)]×100.
 
     Maximum and spontaneous release are determined by incubating the labeled targets with 1% Triton X-100 and media alone, respectively. A positive culture is defined as one in which the specific lysis (sample−background) is 10% or higher in the case of individual wells and is 15% or more at the two highest E:T ratios when expanded cultures are assayed. 
     In Situ Measurement of Human IFNγ Production as an Indicator of Peptide-Specific and Endogenous Recognition 
     Immulon 2 plates are coated with mouse anti-human IFNγ monoclonal antibody (4 μg/ml 0.1M NaHCO3, pH8.2) overnight at 4° C. The plates are washed with Ca2+, Mg2+-free PBS/0.05% Tween 20 and blocked with PBS/10% FCS for two hours, after which the CTLs (100 μl/well) and targets (100 μl/well) are added to each well, leaving empty wells for the standards and blanks (which received media only). The target cells, either peptide-pulsed or endogenous targets, are used at a concentration of 1×106 cells/ml. The plates are incubated for 48 hours at 37° C. with 5% CO2. 
     Recombinant human IFN-gamma is added to the standard wells starting at 400 pg or 1200 pg/100 microliter/well and the plate incubated for two hours at 37° C. The plates are washed and 100 μl of biotinylated mouse anti-human IFN-gamma monoclonal antibody (2 microgram/ml in PBS/3% FCS/0.05% Tween 20) are added and incubated for 2 hours at room temperature. After washing again, 100 microliter HRP-streptavidin (1:4000) are added and the plates incubated for one hour at room temperature. The plates are then washed 6× with wash buffer, 100 microliter/well developing solution (TMB 1:1) are added, and the plates allowed to develop for 5-15 minutes. The reaction is stopped with 50 microliter/well 1M H3PO4 and read at OD450. A culture is considered positive if it measured at least 50 pg of IFN-gamma/well above background and is twice the background level of expression. 
     CTL Expansion. 
     Those cultures that demonstrate specific lytic activity against peptide-pulsed targets and/or tumor targets are expanded over a two week period with anti-CD3. Briefly, 5×104 CD8+ cells are added to a T25 flask containing the following: 1×106 irradiated (4,200 rad) PBMC (autologous or allogeneic) per ml, 2×105 irradiated (8,000 rad) EBV-transformed cells per ml, and OKT3 (anti-CD3) at 30 ng per ml in RPMI-1640 containing 10% (v/v) human AB serum, non-essential amino acids, sodium pyruvate, 25 μM 2-mercaptoethanol, L-glutamine and penicillin/streptomycin. Recombinant human IL2 is added 24 hours later at a final concentration of 200 IU/ml and every three days thereafter with fresh media at 50 IU/ml. The cells are split if the cell concentration exceeds 1×106/ml and the cultures are assayed between days 13 and 15 at E:T ratios of 30, 10, 3 and 1:1 in the 51Cr release assay or at 1×106/ml in the in situ IFNγ assay using the same targets as before the expansion. 
     Cultures are expanded in the absence of anti-CD3+ as follows. Those cultures that demonstrate specific lytic activity against peptide and endogenous targets are selected and 5×104 CD8+ cells are added to a T25 flask containing the following: 1×106 autologous PBMC per ml which have been peptide-pulsed with 10 μg/ml peptide for two hours at 37° C. and irradiated (4,200 rad); 2×105 irradiated (8,000 rad) EBV-transformed cells per ml RPMI-1640 containing 10% (v/v) human AB serum, non-essential AA, sodium pyruvate, 25 mM 2-ME, L-glutamine and gentamicin. 
     Immunogenicity of A2 Supermotif-Bearing Peptides 
     A2-supermotif cross-reactive binding peptides are tested in the cellular assay for the ability to induce peptide-specific CTL in normal individuals. In this analysis, a peptide is typically considered to be an epitope if it induces peptide-specific CTLs in at least individuals, and preferably, also recognizes the endogenously expressed peptide. 
     Immunogenicity can also be confirmed using PBMCs isolated from patients bearing a tumor that expresses 158P3D2. Briefly, PBMCs are isolated from patients, re-stimulated with peptide-pulsed monocytes and assayed for the ability to recognize peptide-pulsed target cells as well as transfected cells endogenously expressing the antigen. 
     Evaluation of A*03/A11 Immunogenicity 
     HLA-A3 supermotif-bearing cross-reactive binding peptides are also evaluated for immunogenicity using methodology analogous for that used to evaluate the immunogenicity of the HLA-A2 supermotif peptides. 
     Evaluation of B7 Immunogenicity 
     Immunogenicity screening of the B7-supertype cross-reactive binding peptides identified as set forth herein are confirmed in a manner analogous to the confirmation of A2- and A3-supermotif-bearing peptides. 
     Peptides bearing other supermotifs/motifs, e.g., HLA-A1, HLA-A24 etc. are also confirmed using similar methodology 
     Example 15 
     Implementation of the Extended Supermotif to Improve the Binding Capacity of Native Epitopes by Creating Analogs 
     HLA motifs and supermotifs (comprising primary and/or secondary residues) are useful in the identification and preparation of highly cross-reactive native peptides, as demonstrated herein. Moreover, the definition of HLA motifs and supermotifs also allows one to engineer highly cross-reactive epitopes by identifying residues within a native peptide sequence which can be analoged to confer upon the peptide certain characteristics, e.g. greater cross-reactivity within the group of HLA molecules that comprise a supertype, and/or greater binding affinity for some or all of those HLA molecules. Examples of analoging peptides to exhibit modulated binding affinity are set forth in this example. 
     Analoging at Primary Anchor Residues 
     Peptide engineering strategies are implemented to further increase the cross-reactivity of the epitopes. For example, the main anchors of A2-supermotif-bearing peptides are altered, for example, to introduce a preferred L, I, V, or M at position 2, and I or V at the C-terminus. 
     To analyze the cross-reactivity of the analog peptides, each engineered analog is initially tested for binding to the prototype A2 supertype allele A*0201, then, if A*0201 binding capacity is maintained, for A2-supertype cross-reactivity. 
     Alternatively, a peptide is confirmed as binding one or all supertype members and then analoged to modulate binding affinity to any one (or more) of the supertype members to add population coverage. 
     The selection of analogs for immunogenicity in a cellular screening analysis is typically further restricted by the capacity of the parent wild type (WT) peptide to bind at least weakly, i.e., bind at an IC50 of 5000 nM or less, to three of more A2 supertype alleles. The rationale for this requirement is that the WT peptides must be present endogenously in sufficient quantity to be biologically relevant. Analoged peptides have been shown to have increased immunogenicity and cross-reactivity by T cells specific for the parent epitope (see, e.g., Parkhurst et al., J. Immunol. 157:2539, 1996; and Pogue et al., Proc. Natl. Acad. Sci. USA 92:8166, 1995). 
     In the cellular screening of these peptide analogs, it is important to confirm that analog-specific CTLs are also able to recognize the wild-type peptide and, when possible, target cells that endogenously express the epitope. 
     Analoging of HLA-A3 and B7-Supermotif-Bearing Peptides 
     Analogs of HLA-A3 supermotif-bearing epitopes are generated using strategies similar to those employed in analoging HLA-A2 supermotif-bearing peptides. For example, peptides binding to 3/5 of the A3-supertype molecules are engineered at primary anchor residues to possess a preferred residue (V, S, M, or A) at position 2. 
     The analog peptides are then tested for the ability to bind A*03 and A*11 (prototype A3 supertype alleles). Those peptides that demonstrate ≦500 nM binding capacity are then confirmed as having A3-supertype cross-reactivity. 
     Similarly to the A2- and A3-motif bearing peptides, peptides binding 3 or more B7-supertype alleles can be improved, where possible, to achieve increased cross-reactive binding or greater binding affinity or binding half life. B7 supermotif-bearing peptides are, for example, engineered to possess a preferred residue (V, I, L, or F) at the C-terminal primary anchor position, as demonstrated by Sidney et al. (J. Immunol. 157:3480-3490, 1996). 
     Analoging at primary anchor residues of other motif and/or supermotif-bearing epitopes is performed in a like manner. 
     The analog peptides are then be confirmed for immunogenicity, typically in a cellular screening assay. Again, it is generally important to demonstrate that analog-specific CTLs are also able to recognize the wild-type peptide and, when possible, targets that endogenously express the epitope. 
     Analoging at Secondary Anchor Residues 
     Moreover, HLA supermotifs are of value in engineering highly cross-reactive peptides and/or peptides that bind HLA molecules with increased affinity by identifying particular residues at secondary anchor positions that are associated with such properties. For example, the binding capacity of a B7 supermotif-bearing peptide with an F residue at position 1 is analyzed. The peptide is then analoged to, for example, substitute L for F at position 1. The analoged peptide is evaluated for increased binding affinity, binding half life and/or increased cross-reactivity. Such a procedure identifies analoged peptides with enhanced properties. 
     Engineered analogs with sufficiently improved binding capacity or cross-reactivity can also be tested for immunogenicity in HLA-B7-transgenic mice, following for example, IFA immunization or lipopeptide immunization. Analoged peptides are additionally tested for the ability to stimulate a recall response using PBMC from patients with 158P3D2-expressing tumors. 
     Other Analoging Strategies 
     Another form of peptide analoging, unrelated to anchor positions, involves the substitution of a cysteine with α-amino butyric acid. Due to its chemical nature, cysteine has the propensity to form disulfide bridges and sufficiently alter the peptide structurally so as to reduce binding capacity. Substitution of α-amino butyric acid for cysteine not only alleviates this problem, but has been shown to improve binding and crossbinding capabilities in some instances (see, e.g., the review by Sette et al., In: Persistent Viral Infections, Eds. R. Ahmed and I. Chen, John Wiley &amp; Sons, England, 1999). 
     Thus, by the use of single amino acid substitutions, the binding properties and/or cross-reactivity of peptide ligands for HLA supertype molecules can be modulated. 
     Example 16 
     Identification and Confirmation of 158P3D2-Derived Sequences with HLA-DR Binding Motifs 
     Peptide epitopes bearing an HLA class II supermotif or motif are identified and confirmed as outlined below using methodology similar to that described for HLA Class I peptides. 
     Selection of HLA-DR-Supermotif-Bearing Epitopes. 
     To identify 158P3D2-derived, HLA class II HTL epitopes, a 158P3D2 antigen is analyzed for the presence of sequences bearing an HLA-DR-motif or supermotif. Specifically, 15-mer sequences are selected comprising a DR-supermotif, comprising a 9-mer core, and three-residue N- and C-terminal flanking regions (15 amino acids total). 
     Protocols for predicting peptide binding to DR molecules have been developed (Southwood et al., J. Immunol. 160:3363-3373, 1998). These protocols, specific for individual DR molecules, allow the scoring, and ranking, of 9-mer core regions. Each protocol not only scores peptide sequences for the presence of DR-supermotif primary anchors (i.e., at position 1 and position 6) within a 9-mer core, but additionally evaluates sequences for the presence of secondary anchors. Using allele-specific selection tables (see, e.g., Southwood et al., ibid.), it has been found that these protocols efficiently select peptide sequences with a high probability of binding a particular DR molecule. Additionally, it has been found that performing these protocols in tandem, specifically those for DR1, DR4w4, and DR7, can efficiently select DR cross-reactive peptides. 
     The 158P3D2-derived peptides identified above are tested for their binding capacity for various common HLA-DR molecules. All peptides are initially tested for binding to the DR molecules in the primary panel: DR1, DR4w4, and DR7. Peptides binding at least two of these three DR molecules are then tested for binding to DR2w2 β1, DR2w2 β2, DR6w19, and DR9 molecules in secondary assays. Finally, peptides binding at least two of the four secondary panel DR molecules, and thus cumulatively at least four of seven different DR molecules, are screened for binding to DR4w15, DR5w11, and DR8w2 molecules in tertiary assays. Peptides binding at least seven of the ten DR molecules comprising the primary, secondary, and tertiary screening assays are considered cross-reactive DR binders. 158P3D2-derived peptides found to bind common HLA-DR alleles are of particular interest. 
     Selection of DR3 Motif Peptides 
     Because HLA-DR3 is an allele that is prevalent in Caucasian, Black, and Hispanic populations, DR3 binding capacity is a relevant criterion in the selection of HTL epitopes. Thus, peptides shown to be candidates may also be assayed for their DR3 binding capacity. However, in view of the binding specificity of the DR3 motif, peptides binding only to DR3 can also be considered as candidates for inclusion in a vaccine formulation. 
     To efficiently identify peptides that bind DR3, target 158P3D2 antigens are analyzed for sequences carrying one of the two DR3-specific binding motifs reported by Geluk et al. (J. Immunol. 152:5742-5748, 1994). The corresponding peptides are then synthesized and confirmed as having the ability to bind DR3 with an affinity of 1 μM or better, i.e., less than 1 μM. Peptides are found that meet this binding criterion and qualify as HLA class II high affinity binders. 
     DR3 binding epitopes identified in this manner are included in vaccine compositions with DR supermotif-bearing peptide epitopes. 
     Similarly to the case of HLA class I motif-bearing peptides, the class II motif-bearing peptides are analoged to improve affinity or cross-reactivity. For example, aspartic acid at position 4 of the 9-mer core sequence is an optimal residue for DR3 binding, and substitution for that residue often improves DR 3 binding. 
     Example 17 
     Immunogenicity of 158P3D2-Derived HTL Epitopes 
     This example determines immunogenic DR supermotif- and DR3 motif-bearing epitopes among those identified using the methodology set forth herein. 
     Immunogenicity of HTL epitopes are confirmed in a manner analogous to the determination of immunogenicity of CTL epitopes, by assessing the ability to stimulate HTL responses and/or by using appropriate transgenic mouse models Immunogenicity is determined by screening for: 1.) in vitro primary induction using normal PBMC or 2.) recall responses from patients who have 158P3D2-expressing tumors. 
     Example 18 
     Calculation of Phenotypic Frequencies of HLA-Supertypes in Various Ethnic Backgrounds to Determine Breadth of Population Coverage 
     This example illustrates the assessment of the breadth of population coverage of a vaccine composition comprised of multiple epitopes comprising multiple supermotifs and/or motifs. 
     In order to analyze population coverage, gene frequencies of HLA alleles are determined. Gene frequencies for each HLA allele are calculated from antigen or allele frequencies utilizing the binomial distribution formulae gf=1−(SQRT(1−af)) (see, e.g., Sidney et al., Human Immunol. 45:79-93, 1996). To obtain overall phenotypic frequencies, cumulative gene frequencies are calculated, and the cumulative antigen frequencies derived by the use of the inverse formula [af=1−(1−Cgf)2]. 
     Where frequency data is not available at the level of DNA typing, correspondence to the serologically defined antigen frequencies is assumed. To obtain total potential supertype population coverage no linkage disequilibrium is assumed, and only alleles confirmed to belong to each of the supertypes are included (minimal estimates). Estimates of total potential coverage achieved by inter-loci combinations are made by adding to the A coverage the proportion of the non-A covered population that could be expected to be covered by the B alleles considered (e.g., tota1=A+B*(1−A)). Confirmed members of the A3-like supertype are A3, All, A31, A*3301, and A*6801. Although the A3-like supertype may also include A34, A66, and A*7401, these alleles were not included in overall frequency calculations. Likewise, confirmed members of the A2-like supertype family are A*0201, A*0202, A*0203, A*0204, A*0205, A*0206, A*0207, A*6802, and A*6901. Finally, the B7-like supertype-confirmed alleles are: B7, B*3501-03, B51, B*5301, B*5401, B*5501-2, B*5601, B*6701, and B*7801 (potentially also B*1401, B*3504-06, B*4201, and B*5602). 
     Population coverage achieved by combining the A2-, A3- and B7-supertypes is approximately 86% in five major ethnic groups. Coverage may be extended by including peptides bearing the A1 and A24 motifs. On average, A1 is present in 12% and A24 in 29% of the population across five different major ethnic groups (Caucasian, North American Black, Chinese, Japanese, and Hispanic). Together, these alleles are represented with an average frequency of 39% in these same ethnic populations. The total coverage across the major ethnicities when A1 and A24 are combined with the coverage of the A2-, A3- and B7-supertype alleles is &gt;95%, see, e.g., Table IV (G). An analogous approach can be used to estimate population coverage achieved with combinations of class II motif-bearing epitopes. 
     Immunogenicity studies in humans (e.g., Bertoni et al., J. Clin. Invest. 100:503, 1997; Doolan et al., Immunity 7:97, 1997; and Threlkeld et al., J. Immunol. 159:1648, 1997) have shown that highly cross-reactive binding peptides are almost always recognized as epitopes. The use of highly cross-reactive binding peptides is an important selection criterion in identifying candidate epitopes for inclusion in a vaccine that is immunogenic in a diverse population. 
     With a sufficient number of epitopes (as disclosed herein and from the art), an average population coverage is predicted to be greater than 95% in each of five major ethnic populations. The game theory Monte Carlo simulation analysis, which is known in the art (see e.g., Osborne, M. J. and Rubinstein, A. “A course in game theory” MIT Press, 1994), can be used to estimate what percentage of the individuals in a population comprised of the Caucasian, North American Black, Japanese, Chinese, and Hispanic ethnic groups would recognize the vaccine epitopes described herein. A preferred percentage is 90%. A more preferred percentage is 95%. 
     Example 19 
     CTL Recognition Of Endogenously Processed Antigens after Priming 
     This example confirms that CTL induced by native or analoged peptide epitopes identified and selected as described herein recognize endogenously synthesized, i.e., native antigens. 
     Effector cells isolated from transgenic mice that are immunized with peptide epitopes, for example HLA-A2 supermotif-bearing epitopes, are re-stimulated in vitro using peptide-coated stimulator cells. Six days later, effector cells are assayed for cytotoxicity and the cell lines that contain peptide-specific cytotoxic activity are further re-stimulated. An additional six days later, these cell lines are tested for cytotoxic activity on  51 Cr labeled Jurkat-A2.1/K b  target cells in the absence or presence of peptide, and also tested on  51 Cr labeled target cells bearing the endogenously synthesized antigen, i.e. cells that are stably transfected with 158P3D2 expression vectors. 
     The results demonstrate that CTL lines obtained from animals primed with peptide epitope recognize endogenously synthesized 158P3D2 antigen. The choice of transgenic mouse model to be used for such an analysis depends upon the epitope(s) that are being evaluated. In addition to HLA-A*0201/K b  transgenic mice, several other transgenic mouse models including mice with human A11, which may also be used to evaluate A3 epitopes, and B7 alleles have been characterized and others (e.g., transgenic mice for HLA-A1 and A24) are being developed. HLA-DR1 and HLA-DR3 mouse models have also been developed, which may be used to evaluate HTL epitopes. 
     Example 20 
     Activity of CTL-HTL Conjugated Epitopes in Transgenic Mice 
     This example illustrates the induction of CTLs and HTLs in transgenic mice, by use of a 158P3D2-derived CTL and HTL peptide vaccine compositions. The vaccine composition used herein comprise peptides to be administered to a patient with a 158P3D2-expressing tumor. The peptide composition can comprise multiple CTL and/or HTL epitopes. The epitopes are identified using methodology as described herein. This example also illustrates that enhanced immunogenicity can be achieved by inclusion of one or more HTL epitopes in a CTL vaccine composition; such a peptide composition can comprise an HTL epitope conjugated to a CTL epitope. The CTL epitope can be one that binds to multiple HLA family members at an affinity of 500 nM or less, or analogs of that epitope. The peptides may be lipidated, if desired. 
     Immunization Procedures: 
     Immunization of transgenic mice is performed as described (Alexander et al.,  J. Immunol.  159:4753-4761, 1997). For example, A2/K b  mice, which are transgenic for the human HLA A2.1 allele and are used to confirm the immunogenicity of HLA-A*0201 motif- or HLA-A2 supermotif-bearing epitopes, and are primed subcutaneously (base of the tail) with a 0.1 ml of peptide in Incomplete Freund&#39;s Adjuvant, or if the peptide composition is a lipidated CTL/HTL conjugate, in DMSO/saline, or if the peptide composition is a polypeptide, in PBS or Incomplete Freund&#39;s Adjuvant. Seven days after priming, splenocytes obtained from these animals are restimulated with syngenic irradiated LPS-activated lymphoblasts coated with peptide. 
     Cell Lines: 
     Target cells for peptide-specific cytotoxicity assays are Jurkat cells transfected with the HLA-A2.1/K b  chimeric gene (e.g., Vitiello et al.,  J. Exp. Med.  173:1007, 1991) 
     In Vitro CTL Activation: 
     One week after priming, spleen cells (30×10 6  cells/flask) are co-cultured at 37° C. with syngeneic, irradiated (3000 rads), peptide coated lymphoblasts (10×10 6  cells/flask) in 10 ml of culture medium/T25 flask. After six days, effector cells are harvested and assayed for cytotoxic activity. 
     Assay for Cytotoxic Activity: 
     Target cells (1.0 to 1.5×10 6 ) are incubated at 37° C. in the presence of 200 μl of  51 Cr. After 60 minutes, cells are washed three times and resuspended in R10 medium. Peptide is added where required at a concentration of 1 μg/ml. For the assay, 10 4 51 Cr-labeled target cells are added to different concentrations of effector cells (final volume of 200 μl) in U-bottom 96-well plates. After a six hour incubation period at 37° C., a 0.1 ml aliquot of supernatant is removed from each well and radioactivity is determined in a Micromedic automatic gamma counter. The percent specific lysis is determined by the formula: percent specific release=100×(experimental release−spontaneous release)/(maximum release−spontaneous release). To facilitate comparison between separate CTL assays run under the same conditions, %  51 Cr release data is expressed as lytic units/10 6  cells. One lytic unit is arbitrarily defined as the number of effector cells required to achieve 30% lysis of 10,000 target cells in a six hour  51 Cr release assay. To obtain specific lytic units/10 6 , the lytic units/10 6  obtained in the absence of peptide is subtracted from the lytic units/10 6  obtained in the presence of peptide. For example, if 30%  51 Cr release is obtained at the effector (E):target (T) ratio of 50:1 (i.e., 5×10 5  effector cells for 10,000 targets) in the absence of peptide and 5:1 (i.e., 5×10 4  effector cells for 10,000 targets) in the presence of peptide, the specific lytic units would be: R1/50,000)−(1/500,000)1×10 6 =18 LU. 
     The results are analyzed to assess the magnitude of the CTL responses of animals injected with the immunogenic CTL/HTL conjugate vaccine preparation and are compared to the magnitude of the CTL response achieved using, for example, CTL epitopes as outlined above in the Example entitled “Confirmation of Immunogenicity.” Analyses similar to this may be performed to confirm the immunogenicity of peptide conjugates containing multiple CTL epitopes and/or multiple HTL epitopes. In accordance with these procedures, it is found that a CTL response is induced, and concomitantly that an HTL response is induced upon administration of such compositions. 
     Example 21 
     Selection of CTL and HTL Epitopes for Inclusion in a 158P3D2-Specific Vaccine 
     This example illustrates a procedure for selecting peptide epitopes for vaccine compositions of the invention. The peptides in the composition can be in the form of a nucleic acid sequence, either single or one or more sequences (i.e., minigene) that encodes peptide(s), or can be single and/or polyepitopic peptides. 
     The following principles are utilized when selecting a plurality of epitopes for inclusion in a vaccine composition. Each of the following principles is balanced in order to make the selection. 
     Epitopes are selected which, upon administration, mimic immune responses that are correlated with 158P3D2 clearance. The number of epitopes used depends on observations of patients who spontaneously clear 158P3D2. For example, if it has been observed that patients who spontaneously clear 158P3D2-expressing cells generate an immune response to at least three (3) epitopes from 158P3D2 antigen, then at least three epitopes should be included for HLA class I. A similar rationale is used to determine HLA class II epitopes. 
     Epitopes are often selected that have a binding affinity of an IC 50  of 500 nM or less for an HLA class I molecule, or for class II, an IC 50  of 1000 nM or less; or HLA Class I peptides with high binding scores from the BIMAS web site, at URL bimas.dcrt.nih.gov/. 
     In order to achieve broad coverage of the vaccine through out a diverse population, sufficient supermotif bearing peptides, or a sufficient array of allele-specific motif bearing peptides, are selected to give broad population coverage. In one embodiment, epitopes are selected to provide at least 80% population coverage. A Monte Carlo analysis, a statistical evaluation known in the art, can be employed to assess breadth, or redundancy, of population coverage. 
     When creating polyepitopic compositions, or a minigene that encodes same, it is typically desirable to generate the smallest peptide possible that encompasses the epitopes of interest. The principles employed are similar, if not the same, as those employed when selecting a peptide comprising nested epitopes. For example, a protein sequence for the vaccine composition is selected because it has maximal number of epitopes contained within the sequence, i.e., it has a high concentration of epitopes. Epitopes may be nested or overlapping (i.e., frame shifted relative to one another). For example, with overlapping epitopes, two 9-mer epitopes and one 10-mer epitope can be present in a 10 amino acid peptide. Each epitope can be exposed and bound by an HLA molecule upon administration of such a peptide. A multi-epitopic, peptide can be generated synthetically, recombinantly, or via cleavage from the native source. Alternatively, an analog can be made of this native sequence, whereby one or more of the epitopes comprise substitutions that alter the cross-reactivity and/or binding affinity properties of the polyepitopic peptide. Such a vaccine composition is administered for therapeutic or prophylactic purposes. This embodiment provides for the possibility that an as yet undiscovered aspect of immune system processing will apply to the native nested sequence and thereby facilitate the production of therapeutic or prophylactic immune response-inducing vaccine compositions. Additionally such an embodiment provides for the possibility of motif-bearing epitopes for an HLA makeup that is presently unknown. Furthermore, this embodiment (absent the creating of any analogs) directs the immune response to multiple peptide sequences that are actually present in 158P3D2, thus avoiding the need to evaluate any junctional epitopes. Lastly, the embodiment provides an economy of scale when producing nucleic acid vaccine compositions. Related to this embodiment, computer programs can be derived in accordance with principles in the art, which identify in a target sequence, the greatest number of epitopes per sequence length. 
     A vaccine composition comprised of selected peptides, when administered, is safe, efficacious, and elicits an immune response similar in magnitude to an immune response that controls or clears cells that bear or overexpress 158P3D2. 
     Example 22 
     Construction of “Minigene” Multi-Epitope DNA Plasmids 
     This example discusses the construction of a minigene expression plasmid. Minigene plasmids may, of course, contain various configurations of B cell, CTL and/or HTL epitopes or epitope analogs as described herein. 
     A minigene expression plasmid typically includes multiple CTL and HTL peptide epitopes. In the present example, HLA-A2, -A3, -B7 supermotif-bearing peptide epitopes and HLA-A1 and -A24 motif-bearing peptide epitopes are used in conjunction with DR supermotif-bearing epitopes and/or DR3 epitopes. HLA class I supermotif or motif-bearing peptide epitopes derived 158P3D2, are selected such that multiple supermotifs/motifs are represented to ensure broad population coverage. Similarly, HLA class II epitopes are selected from 158P3D2 to provide broad population coverage, i.e. both HLA DR-1-4-7 supermotif-bearing epitopes and HLA DR-3 motif-bearing epitopes are selected for inclusion in the minigene construct. The selected CTL and HTL epitopes are then incorporated into a minigene for expression in an expression vector. 
     Such a construct may additionally include sequences that direct the HTL epitopes to the endoplasmic reticulum. For example, the Ii protein may be fused to one or more HTL epitopes as described in the art, wherein the CLIP sequence of the Ii protein is removed and replaced with an HLA class II epitope sequence so that HLA class II epitope is directed to the endoplasmic reticulum, where the epitope binds to an HLA class II molecules. 
     This example illustrates the methods to be used for construction of a minigene-bearing expression plasmid. Other expression vectors that may be used for minigene compositions are available and known to those of skill in the art. 
     The minigene DNA plasmid of this example contains a consensus Kozak sequence and a consensus murine kappa Ig-light chain signal sequence followed by CTL and/or HTL epitopes selected in accordance with principles disclosed herein. The sequence encodes an open reading frame fused to the Myc and His antibody epitope tag coded for by the pcDNA 3.1 Myc-His vector. 
     Overlapping oligonucleotides that can, for example, average about 70 nucleotides in length with 15 nucleotide overlaps, are synthesized and HPLC-purified. The oligonucleotides encode the selected peptide epitopes as well as appropriate linker nucleotides, Kozak sequence, and signal sequence. The final multiepitope minigene is assembled by extending the overlapping oligonucleotides in three sets of reactions using PCR. A Perkin/Elmer 9600 PCR machine is used and a total of 30 cycles are performed using the following conditions: 95° C. for 15 sec, annealing temperature (5° below the lowest calculated Tm of each primer pair) for 30 sec, and 72° C. for 1 min 
     For example, a minigene is prepared as follows. For a first PCR reaction, 5 μg of each of two oligonucleotides are annealed and extended: In an example using eight oligonucleotides, i.e., four pairs of primers, oligonucleotides 1+2, 3+4, 5+6, and 7+8 are combined in 100 μl reactions containing Pfu polymerase buffer (1×=10 mM KCL, 10 mM (NH4) 2 SO 4 , 20 mM Tris-chloride, pH 8.75, 2 mM MgSO 4 , 0.1% Triton X-100, 100 μg/ml BSA), 0.25 mM each dNTP, and 2.5 U of Pfu polymerase. The full-length dimer products are gel-purified, and two reactions containing the product of 1+2 and 3+4, and the product of 5+6 and 7+8 are mixed, annealed, and extended for 10 cycles. Half of the two reactions are then mixed, and 5 cycles of annealing and extension carried out before flanking primers are added to amplify the full length product. The full-length product is gel-purified and cloned into pCR-blunt (Invitrogen) and individual clones are screened by sequencing. 
     Example 23 
     The Plasmid Construct and the Degree to which it Induces Immunogenicity 
     The degree to which a plasmid construct, for example a plasmid constructed in accordance with the previous Example, is able to induce immunogenicity is confirmed in vitro by determining epitope presentation by APC following transduction or transfection of the APC with an epitope-expressing nucleic acid construct. Such a study determines “antigenicity” and allows the use of human APC. The assay determines the ability of the epitope to be presented by the APC in a context that is recognized by a T cell by quantifying the density of epitope-HLA class I complexes on the cell surface. Quantitation can be performed by directly measuring the amount of peptide eluted from the APC (see, e.g., Sijts et al.,  J. Immunol.  156:683-692, 1996; Demotz et al., Nature 342:682-684, 1989); or the number of peptide-HLA class I complexes can be estimated by measuring the amount of lysis or lymphokine release induced by diseased or transfected target cells, and then determining the concentration of peptide necessary to obtain equivalent levels of lysis or lymphokine release (see, e.g., Kageyama et al.,  J. Immunol.  154:567-576, 1995). 
     Alternatively, immunogenicity is confirmed through in vivo injections into mice and subsequent in vitro assessment of CTL and HTL activity, which are analyzed using cytotoxicity and proliferation assays, respectively, as detailed e.g., in Alexander et al.,  Immunity  1:751-761, 1994. 
     For example, to confirm the capacity of a DNA minigene construct containing at least one HLA-A2 supermotif peptide to induce CTLs in vivo, HLA-A2.1/K b  transgenic mice, for example, are immunized intramuscularly with 100 μg of naked cDNA. As a means of comparing the level of CTLs induced by cDNA immunization, a control group of animals is also immunized with an actual peptide composition that comprises multiple epitopes synthesized as a single polypeptide as they would be encoded by the minigene. 
     Splenocytes from immunized animals are stimulated twice with each of the respective compositions (peptide epitopes encoded in the minigene or the polyepitopic peptide), then assayed for peptide-specific cytotoxic activity in a  51 Cr release assay. The results indicate the magnitude of the CTL response directed against the A2-restricted epitope, thus indicating the in vivo immunogenicity of the minigene vaccine and polyepitopic vaccine. 
     It is, therefore, found that the minigene elicits immune responses directed toward the HLA-A2 supermotif peptide epitopes as does the polyepitopic peptide vaccine. A similar analysis is also performed using other HLA-A3 and HLA-B7 transgenic mouse models to assess CTL induction by HLA-A3 and HLA-B7 motif or supermotif epitopes, whereby it is also found that the minigene elicits appropriate immune responses directed toward the provided epitopes. 
     To confirm the capacity of a class II epitope-encoding minigene to induce HTLs in vivo, DR transgenic mice, or for those epitopes that cross react with the appropriate mouse MHC molecule, I-A b -restricted mice, for example, are immunized intramuscularly with 100 μg of plasmid DNA. As a means of comparing the level of HTLs induced by DNA immunization, a group of control animals is also immunized with an actual peptide composition emulsified in complete Freund&#39;s adjuvant. CD4+ T cells, i.e. HTLs, are purified from splenocytes of immunized animals and stimulated with each of the respective compositions (peptides encoded in the minigene). The HTL response is measured using a  3 H-thymidine incorporation proliferation assay, (see, e.g., Alexander et al. Immunity 1:751-761, 1994). The results indicate the magnitude of the HTL response, thus demonstrating the in vivo immunogenicity of the minigene. 
     DNA minigenes, constructed as described in the previous Example, can also be confirmed as a vaccine in combination with a boosting agent using a prime boost protocol. The boosting agent can consist of recombinant protein (e.g., Barnett et al.,  Aids Res. and Human Retroviruses  14, Supplement 3:S299-S309, 1998) or recombinant vaccinia, for example, expressing a minigene or DNA encoding the complete protein of interest (see, e.g., Hanke et al.,  Vaccine  16:439-445, 1998; Sedegah et al.,  Proc. Natl. Acad. Sci. USA  95:7648-53, 1998; Hanke and McMichael,  Immunol. Letters  66:177-181, 1999; and Robinson et al.,  Nature Med.  5:526-34, 1999). 
     For example, the efficacy of the DNA minigene used in a prime boost protocol is initially evaluated in transgenic mice. In this example, A2.1/K b  transgenic mice are immunized IM with 100 μg of a DNA minigene encoding the immunogenic peptides including at least one 
     HLA-A2 supermotif-bearing peptide. After an incubation period (ranging from 3-9 weeks), the mice are boosted IP with 10 7  pfu/mouse of a recombinant vaccinia virus expressing the same sequence encoded by the DNA minigene. Control mice are immunized with 100 μg of DNA or recombinant vaccinia without the minigene sequence, or with DNA encoding the minigene, but without the vaccinia boost. After an additional incubation period of two weeks, splenocytes from the mice are immediately assayed for peptide-specific activity in an ELISPOT assay. Additionally, splenocytes are stimulated in vitro with the A2-restricted peptide epitopes encoded in the minigene and recombinant vaccinia, then assayed for peptide-specific activity in an alpha, beta and/or gamma IFN ELISA. 
     It is found that the minigene utilized in a prime-boost protocol elicits greater immune responses toward the HLA-A2 supermotif peptides than with DNA alone. Such an analysis can also be performed using HLA-A11 or HLA-B7 transgenic mouse models to assess CTL induction by HLA-A3 or HLA-B7 motif or supermotif epitopes. The use of prime boost protocols in humans is described below in the Example entitled “Induction of CTL Responses Using a Prime Boost Protocol.” 
     Example 24 
     Peptide Compositions for Prophylactic Uses 
     Vaccine compositions of the present invention can be used to prevent 158P3D2 expression in persons who are at risk for tumors that bear this antigen. For example, a polyepitopic peptide epitope composition (or a nucleic acid comprising the same) containing multiple CTL and HTL epitopes such as those selected in the above Examples, which are also selected to target greater than 80% of the population, is administered to individuals at risk for a 158P3D2-associated tumor. 
     For example, a peptide-based composition is provided as a single polypeptide that encompasses multiple epitopes. The vaccine is typically administered in a physiological solution that comprises an adjuvant, such as Incomplete Freunds Adjuvant. The dose of peptide for the initial immunization is from about 1 to about 50,000 μg, generally 100-5,000 μg, for a 70 kg patient. The initial administration of vaccine is followed by booster dosages at 4 weeks followed by evaluation of the magnitude of the immune response in the patient, by techniques that determine the presence of epitope-specific CTL populations in a PBMC sample. Additional booster doses are administered as required. The composition is found to be both safe and efficacious as a prophylaxis against 158P3D2-associated disease. 
     Alternatively, a composition typically comprising transfecting agents is used for the administration of a nucleic acid-based vaccine in accordance with methodologies known in the art and disclosed herein. 
     Example 25 
     Polyepitopic Vaccine Compositions Derived from Native 158P3D2 Sequences 
     A native 158P3D2 polyprotein sequence is analyzed, preferably using computer algorithms defined for each class I and/or class II supermotif or motif, to identify “relatively short” regions of the polyprotein that comprise multiple epitopes. The “relatively short” regions are preferably less in length than an entire native antigen. This relatively short sequence that contains multiple distinct or overlapping, “nested” epitopes can be used to generate a minigene construct. The construct is engineered to express the peptide, which corresponds to the native protein sequence. The “relatively short” peptide is generally less than 250 amino acids in length, often less than 100 amino acids in length, preferably less than 75 amino acids in length, and more preferably less than 50 amino acids in length. The protein sequence of the vaccine composition is selected because it has maximal number of epitopes contained within the sequence, i.e., it has a high concentration of epitopes. As noted herein, epitope motifs may be nested or overlapping (i.e., frame shifted relative to one another). For example, with overlapping epitopes, two 9-mer epitopes and one 10-mer epitope can be present in a 10 amino acid peptide. Such a vaccine composition is administered for therapeutic or prophylactic purposes. 
     The vaccine composition will include, for example, multiple CTL epitopes from 158P3D2 antigen and at least one HTL epitope. This polyepitopic native sequence is administered either as a peptide or as a nucleic acid sequence which encodes the peptide. Alternatively, an analog can be made of this native sequence, whereby one or more of the epitopes comprise substitutions that alter the cross-reactivity and/or binding affinity properties of the polyepitopic peptide. 
     The embodiment of this example provides for the possibility that an as yet undiscovered aspect of immune system processing will apply to the native nested sequence and thereby facilitate the production of therapeutic or prophylactic immune response-inducing vaccine compositions. Additionally, such an embodiment provides for the possibility of motif-bearing epitopes for an HLA makeup(s) that is presently unknown. Furthermore, this embodiment (excluding an analoged embodiment) directs the immune response to multiple peptide sequences that are actually present in native 158P3D2, thus avoiding the need to evaluate any junctional epitopes. Lastly, the embodiment provides an economy of scale when producing peptide or nucleic acid vaccine compositions. 
     Related to this embodiment, computer programs are available in the art which can be used to identify in a target sequence, the greatest number of epitopes per sequence length. 
     Example 26 
     Polyepitopic Vaccine Compositions from Multiple Antigens 
     The 158P3D2 peptide epitopes of the present invention are used in conjunction with epitopes from other target tumor-associated antigens, to create a vaccine composition that is useful for the prevention or treatment of cancer that expresses 158P3D2 and such other antigens. For example, a vaccine composition can be provided as a single polypeptide that incorporates multiple epitopes from 158P3D2 as well as tumor-associated antigens that are often expressed with a target cancer associated with 158P3D2 expression, or can be administered as a composition comprising a cocktail of one or more discrete epitopes. Alternatively, the vaccine can be administered as a minigene construct or as dendritic cells which have been loaded with the peptide epitopes in vitro. 
     Example 27 
     Use of Peptides to Evaluate an Immune Response 
     Peptides of the invention may be used to analyze an immune response for the presence of specific antibodies, CTL or HTL directed to 158P3D2. Such an analysis can be performed in a manner described by Ogg et al.,  Science  279:2103-2106, 1998. In this Example, peptides in accordance with the invention are used as a reagent for diagnostic or prognostic purposes, not as an immunogen. 
     In this example highly sensitive human leukocyte antigen tetrameric complexes (“tetramers”) are used for a cross-sectional analysis of, for example, 158P3D2 HLA-A*0201-specific CTL frequencies from HLA A*0201-positive individuals at different stages of disease or following immunization comprising a 158P3D2 peptide containing an A*0201 motif. Tetrameric complexes are synthesized as described (Musey et al.,  N. Engl. J. Med.  337:1267, 1997). Briefly, purified HLA heavy chain (A*0201 in this example) and β2-microglobulin are synthesized by means of a prokaryotic expression system. The heavy chain is modified by deletion of the transmembrane-cytosolic tail and COOH-terminal addition of a sequence containing a BirA enzymatic biotinylation site. The heavy chain, β2-microglobulin, and peptide are refolded by dilution. The 45-kD refolded product is isolated by fast protein liquid chromatography and then biotinylated by BirA in the presence of biotin (Sigma, St. Louis, Mo.), adenosine 5′ triphosphate and magnesium. Streptavidin-phycoerythrin conjugate is added in a 1:4 molar ratio, and the tetrameric product is concentrated to 1 mg/ml. The resulting product is referred to as tetramer-phycoerythrin. 
     For the analysis of patient blood samples, approximately one million PBMCs are centrifuged at 300 g for 5 minutes and resuspended in 50 μl of cold phosphate-buffered saline. Tri-color analysis is performed with the tetramer-phycoerythrin, along with anti-CD8-Tricolor, and anti-CD38. The PBMCs are incubated with tetramer and antibodies on ice for 30 to 60 min and then washed twice before formaldehyde fixation. Gates are applied to contain &gt;99.98% of control samples. Controls for the tetramers include both A*0201-negative individuals and A*0201-positive non-diseased donors. The percentage of cells stained with the tetramer is then determined by flow cytometry. The results indicate the number of cells in the PBMC sample that contain epitope-restricted CTLs, thereby readily indicating the extent of immune response to the 158P3D2 epitope, and thus the status of exposure to 158P3D2, or exposure to a vaccine that elicits a protective or therapeutic response. 
     Example 28 
     Use of Peptide Epitopes to Evaluate Recall Responses 
     The peptide epitopes of the invention are used as reagents to evaluate T cell responses, such as acute or recall responses, in patients. Such an analysis may be performed on patients who have recovered from 158P3D2-associated disease or who have been vaccinated with a 158P3D2 vaccine. 
     For example, the class I restricted CTL response of persons who have been vaccinated may be analyzed. The vaccine may be any 158P3D2 vaccine. PBMC are collected from vaccinated individuals and HLA typed. Appropriate peptide epitopes of the invention that, optimally, bear supermotifs to provide cross-reactivity with multiple HLA supertype family members, are then used for analysis of samples derived from individuals who bear that HLA type. 
     PBMC from vaccinated individuals are separated on Ficoll-Histopaque density gradients (Sigma Chemical Co., St. Louis, Mo.), washed three times in HBSS (GIBCO Laboratories), resuspended in RPMI-1640 (GIBCO Laboratories) supplemented with L-glutamine (2 mM), penicillin (50 U/ml), streptomycin (50 μg/ml), and Hepes (10 mM) containing 10% heat-inactivated human AB serum (complete RPMI) and plated using microculture formats. A synthetic peptide comprising an epitope of the invention is added at 10 μg/ml to each well and HBV core 128-140 epitope is added at 1 μg/ml to each well as a source of T cell help during the first week of stimulation. 
     In the microculture format, 4×10 5  PBMC are stimulated with peptide in 8 replicate cultures in 96-well round bottom plate in 100 μl/well of complete RPMI. On days 3 and 10, 100 μl of complete RPMI and 20 U/ml final concentration of rIL-2 are added to each well. On day 7 the cultures are transferred into a 96-well flat-bottom plate and restimulated with peptide, rIL-2 and 10 5  irradiated (3,000 rad) autologous feeder cells. The cultures are tested for cytotoxic activity on day 14. A positive CTL response requires two or more of the eight replicate cultures to display greater than 10% specific  51 Cr release, based on comparison with non-diseased control subjects as previously described (Rehermann, et al.,  Nature Med.  2:1104, 1108, 1996; Rehermann et al.,  J. Clin. Invest.  97:1655-1665, 1996; and Rehermann et al.  J. Clin. Invest.  98:1432-1440, 1996). 
     Target cell lines are autologous and allogeneic EBV-transformed B-LCL that are either purchased from the American Society for Histocompatibility and Immunogenetics (ASHI, Boston, Mass.) or established from the pool of patients as described (Guilhot, et al.  J. Virol.  66:2670-2678, 1992). 
     Cytotoxicity assays are performed in the following manner. Target cells consist of either allogeneic HLA-matched or autologous EBV-transformed B lymphoblastoid cell line that are incubated overnight with the synthetic peptide epitope of the invention at 10 μM, and labeled with 100 μCi of  51 Cr (Amersham Corp., Arlington Heights, Ill.) for 1 hour after which they are washed four times with HBSS. 
     Cytolytic activity is determined in a standard 4-h, split well  51 Cr release assay using U-bottomed 96 well plates containing 3,000 targets/well. Stimulated PBMC are tested at effector/target (E/T) ratios of 20-50:1 on day 14. Percent cytotoxicity is determined from the formula: 100×[(experimental release-spontaneous release)/maximum release-spontaneous release)]. Maximum release is determined by lysis of targets by detergent (2% Triton X-100; Sigma Chemical Co., St. Louis, Mo.). Spontaneous release is &lt;25% of maximum release for all experiments. 
     The results of such an analysis indicate the extent to which HLA-restricted CTL populations have been stimulated by previous exposure to 158P3D2 or a 158P3D2 vaccine. 
     Similarly, Class II restricted HTL responses may also be analyzed. Purified PBMC are cultured in a 96-well flat bottom plate at a density of 1.5×10 5  cells/well and are stimulated with 10 μg/ml synthetic peptide of the invention, whole 158P3D2 antigen, or PHA. Cells are routinely plated in replicates of 4-6 wells for each condition. After seven days of culture, the medium is removed and replaced with fresh medium containing 10 U/ml IL-2. Two days later, 1 μCi  3 H-thymidine is added to each well and incubation is continued for an additional 18 hours. Cellular DNA is then harvested on glass fiber mats and analyzed for  3 H-thymidine incorporation. Antigen-specific T cell proliferation is calculated as the ratio of  3 H-thymidine incorporation in the presence of antigen divided by the  3 H-thymidine incorporation in the absence of antigen. 
     Example 29 
     Induction of Specific CTL Response in Humans 
     A human clinical trial for an immunogenic composition comprising CTL and HTL epitopes of the invention is set up as an IND Phase I, dose escalation study and carried out as a randomized, double-blind, placebo-controlled trial. Such a trial is designed, for example, as follows: 
     A total of about 27 individuals are enrolled and divided into 3 groups: 
     Group I: 3 subjects are injected with placebo and 6 subjects are injected with 5 μg of peptide composition; 
     Group II: 3 subjects are injected with placebo and 6 subjects are injected with 50 μg peptide composition; 
     Group III: 3 subjects are injected with placebo and 6 subjects are injected with 500 μg of peptide composition. 
     After 4 weeks following the first injection, all subjects receive a booster inoculation at the same dosage. 
     The endpoints measured in this study relate to the safety and tolerability of the peptide composition as well as its immunogenicity. Cellular immune responses to the peptide composition are an index of the intrinsic activity of this the peptide composition, and can therefore be viewed as a measure of biological efficacy. The following summarize the clinical and laboratory data that relate to safety and efficacy endpoints. 
     Safety: The incidence of adverse events is monitored in the placebo and drug treatment group and assessed in terms of degree and reversibility. 
     Evaluation of Vaccine Efficacy: For evaluation of vaccine efficacy, subjects are bled before and after injection. Peripheral blood mononuclear cells are isolated from fresh heparinized blood by Ficoll-Hypaque density gradient centrifugation, aliquoted in freezing media and stored frozen. Samples are assayed for CTL and HTL activity. 
     The vaccine is found to be both safe and efficacious. 
     Example 30 
     Phase II Trials in Patients Expressing 158P3D2 
     Phase II trials are performed to study the effect of administering the CTL-HTL peptide compositions to patients having cancer that expresses 158P3D2. The main objectives of the trial are to determine an effective dose and regimen for inducing CTLs in cancer patients that express 158P3D2, to establish the safety of inducing a CTL and HTL response in these patients, and to see to what extent activation of CTLs improves the clinical picture of these patients, as manifested, e.g., by the reduction and/or shrinking of lesions. Such a study is designed, for example, as follows: 
     The studies are performed in multiple centers. The trial design is an open-label, uncontrolled, dose escalation protocol wherein the peptide composition is administered as a single dose followed six weeks later by a single booster shot of the same dose. The dosages are 50, 500 and 5,000 micrograms per injection. Drug-associated adverse effects (severity and reversibility) are recorded. 
     There are three patient groupings. The first group is injected with 50 micrograms of the peptide composition and the second and third groups with 500 and 5,000 micrograms of peptide composition, respectively. The patients within each group range in age from 21-65 and represent diverse ethnic backgrounds. All of them have a tumor that expresses 158P3D2. 
     Clinical manifestations or antigen-specific T-cell responses are monitored to assess the effects of administering the peptide compositions. The vaccine composition is found to be both safe and efficacious in the treatment of 158P3D2-associated disease. 
     Example 31 
     Induction of CTL Responses Using a Prime Boost Protocol 
     A prime boost protocol similar in its underlying principle to that used to confirm the efficacy of a DNA vaccine in transgenic mice, such as described above in the Example entitled “The Plasmid Construct and the Degree to Which It Induces Immunogenicity,” can also be used for the administration of the vaccine to humans. Such a vaccine regimen can include an initial administration of, for example, naked DNA followed by a boost using recombinant virus encoding the vaccine, or recombinant protein/polypeptide or a peptide mixture administered in an adjuvant. 
     For example, the initial immunization may be performed using an expression vector, such as that constructed in the Example entitled “Construction of “Minigene” Multi-Epitope DNA Plasmids” in the form of naked nucleic acid administered IM (or SC or ID) in the amounts of 0.5-5 mg at multiple sites. The nucleic acid (0.1 to 1000 μg) can also be administered using a gene gun. Following an incubation period of 3-4 weeks, a booster dose is then administered. The booster can be recombinant fowlpox virus administered at a dose of 5-10 7  to 5×10 9  pfu. An alternative recombinant virus, such as an MVA, canarypox, adenovirus, or adeno-associated virus, can also be used for the booster, or the polyepitopic protein or a mixture of the peptides can be administered. For evaluation of vaccine efficacy, patient blood samples are obtained before immunization as well as at intervals following administration of the initial vaccine and booster doses of the vaccine. Peripheral blood mononuclear cells are isolated from fresh heparinized blood by Ficoll-Hypaque density gradient centrifugation, aliquoted in freezing media and stored frozen. Samples are assayed for CTL and HTL activity. 
     Analysis of the results indicates that a magnitude of response sufficient to achieve a therapeutic or protective immunity against 158P3D2 is generated. 
     Example 32 
     Administration of Vaccine Compositions Using Dendritic Cells (DC) 
     Vaccines comprising peptide epitopes of the invention can be administered using APCs, or “professional” APCs such as DC. In this example, peptide-pulsed DC are administered to a patient to stimulate a CTL response in vivo. In this method, dendritic cells are isolated, expanded, and pulsed with a vaccine comprising peptide CTL and HTL epitopes of the invention. The dendritic cells are infused back into the patient to elicit CTL and HTL responses in vivo. The induced CTL and HTL then destroy or facilitate destruction, respectively, of the target cells that bear the 158P3D2 protein from which the epitopes in the vaccine are derived. 
     For example, a cocktail of epitope-comprising peptides is administered ex vivo to PBMC, or isolated DC therefrom. A pharmaceutical to facilitate harvesting of DC can be used, such as Progenipoietin™ (Monsanto, St. Louis, Mo.) or GM-CSF/IL-4. After pulsing the DC with peptides, and prior to reinfusion into patients, the DC are washed to remove unbound peptides. 
     As appreciated clinically, and readily determined by one of skill based on clinical outcomes, the number of DC reinfused into the patient can vary (see, e.g.,  Nature Med.  4:328, 1998 ; Nature Med.  2:52, 1996 and Prostate 32:272, 1997). Although 2-50×10 6  DC per patient are typically administered, larger number of DC, such as 10 7  or 10 8  can also be provided. Such cell populations typically contain between 50-90% DC. 
     In some embodiments, peptide-loaded PBMC are injected into patients without purification of the DC. For example, PBMC generated after treatment with an agent such as Progenipoietin™ are injected into patients without purification of the DC. The total number of PBMC that are administered often ranges from 10 8  to 10 10 . Generally, the cell doses injected into patients is based on the percentage of DC in the blood of each patient, as determined, for example, by immunofluorescence analysis with specific anti-DC antibodies. Thus, for example, if Progenipoietin™ mobilizes 2% DC in the peripheral blood of a given patient, and that patient is to receive 5×10 6  DC, then the patient will be injected with a total of 2.5×10 8  peptide-loaded PBMC. The percent DC mobilized by an agent such as Progenipoietin™ is typically estimated to be between 2-10%, but can vary as appreciated by one of skill in the art. 
     Ex Vivo Activation of CTL/HTL Responses 
     Alternatively, ex vivo CTL or HTL responses to 158P3D2 antigens can be induced by incubating, in tissue culture, the patient&#39;s, or genetically compatible, CTL or HTL precursor cells together with a source of APC, such as DC, and immunogenic peptides. After an appropriate incubation time (typically about 7-28 days), in which the precursor cells are activated and expanded into effector cells, the cells are infused into the patient, where they will destroy (CTL) or facilitate destruction (HTL) of their specific target cells, i.e., tumor cells. 
     Example 33 
     An Alternative Method of Identifying and Confirming Motif-Bearing Peptides 
     Another method of identifying and confirming motif-bearing peptides is to elute them from cells bearing defined MHC molecules. For example, EBV transformed B cell lines used for tissue typing have been extensively characterized to determine which HLA molecules they express. In certain cases these cells express only a single type of HLA molecule. These cells can be transfected with nucleic acids that express the antigen of interest, e.g. 158P3D2. Peptides produced by endogenous antigen processing of peptides produced as a result of transfection will then bind to HLA molecules within the cell and be transported and displayed on the cell&#39;s surface. Peptides are then eluted from the HLA molecules by exposure to mild acid conditions and their amino acid sequence determined, e.g., by mass spectral analysis (e.g., Kubo et al.,  J. Immunol.  152:3913, 1994). Because the majority of peptides that bind a particular HLA molecule are motif-bearing, this is an alternative modality for obtaining the motif-bearing peptides correlated with the particular HLA molecule expressed on the cell. 
     Alternatively, cell lines that do not express endogenous HLA molecules can be transfected with an expression construct encoding a single HLA allele. These cells can then be used as described, i.e., they can then be transfected with nucleic acids that encode 158P3D2 to isolate peptides corresponding to 158P3D2 that have been presented on the cell surface. Peptides obtained from such an analysis will bear motif(s) that correspond to binding to the single HLA allele that is expressed in the cell. 
     As appreciated by one in the art, one can perform a similar analysis on a cell bearing more than one HLA allele and subsequently determine peptides specific for each HLA allele expressed. Moreover, one of skill would also recognize that means other than transfection, such as loading with a protein antigen, can be used to provide a source of antigen to the cell. 
     Example 34 
     Complementary Polynucleotides 
     Sequences complementary to the 158P3D2-encoding sequences or any parts thereof, are used to detect, decrease, or inhibit expression of naturally occurring 158P3D2. Although use of oligonucleotides comprising from about 15 to 30 base pairs is described, essentially the same procedure is used with smaller or with larger sequence fragments. Appropriate oligonucleotides are designed using, e.g., OLIGO 4.06 software (National Biosciences) and the coding sequence of 158P3D2. To inhibit transcription, a complementary oligonucleotide is designed from the most unique 5′ sequence and used to prevent promoter binding to the coding sequence. To inhibit translation, a complementary oligonucleotide is designed to prevent ribosomal binding to a 158P3D2-encoding transcript. 
     Example 35 
     Purification of Naturally-Occurring or Recombinant 158P3D2 Using 158P3D2-Specific Antibodies 
     Naturally occurring or recombinant 158P3D2 is substantially purified by immunoaffinity chromatography using antibodies specific for 158P3D2. An immunoaffinity column is constructed by covalently coupling anti-158P3D2 antibody to an activated chromatographic resin, such as CNBr-activated SEPHAROSE (Amersham Pharmacia Biotech). After the coupling, the resin is blocked and washed according to the manufacturer&#39;s instructions. 
     Media containing 158P3D2 are passed over the immunoaffinity column, and the column is washed under conditions that allow the preferential absorbance of 158P3D2 (e.g., high ionic strength buffers in the presence of detergent). The column is eluted under conditions that disrupt antibody/158P3D2 binding (e.g., a buffer of pH 2 to pH 3, or a high concentration of a chaotrope, such as urea or thiocyanate ion), and GCR.P is collected. 
     Example 36 
     Identification of Molecules which Interact with 158P3D2 
     158P3D2, or biologically active fragments thereof, are labeled with 121 l Bolton-Hunter reagent. (See, e.g., Bolton et al. (1973) Biochem. J. 133:529.) Candidate molecules previously arrayed in the wells of a multi-well plate are incubated with the labeled 158P3D2, washed, and any wells with labeled 158P3D2 complex are assayed. Data obtained using different concentrations of 158P3D2 are used to calculate values for the number, affinity, and association of 158P3D2 with the candidate molecules. 
     Example 37 
     In Vivo Assay for 158P3D2 Tumor Growth Promotion In Vivo Assay of 3T3 Cell Growth by Recombinant Expression of 158P3D2 
     To address the determination of 158P3D2 to accelerate the growth of non-tumorigenic cells in an in vivo mouse model, non-transformed 3T3 cells are prepared by infection with either a virus containing an empty vector control (Neo gene alone) or with a vector containing the 158P3D2 full-length gene. 3T3 cells are selected for survival in G-418, and expression of 158P3D2 confirmed by Northern blot analysis. To assess the growth of these cells, 1×10 6  158P3D2 expressing 3T3 cells or 1×10 6  Neo control are mixed with Matrigel®, then injected intratibially or subcutaneously in SCID mice and allowed to grow for 30 days. The growth of these cells is assessed on day 30 by visual inspection and by necropsy. The 158P3D2 expressing 3T3 cells show a potent effect in comparison to the 3T3-Neo cells, indicating that the 158P3D2 protein enhanced the growth of the cells in Matrigel®. 158P3D2 promotes the growth of non-tumorigenic cells and provides a growth advantage in vivo that mimics the role of this protein in human malignancies. 
     Example 38 
     158P3D2 Monoclonal Antibody-Mediated Inhibition of Bladder, Lung, Colon and Breast and other Tumors In Vivo 
     The significant expression of 158P3D2 in cancer tissues, together with its restrictive expression in normal tissues makes 158P3D2 a good target for antibody therapy. Similarly, 158P3D2 is a target for T cell-based immunotherapy. Thus, the therapeutic efficacy of anti-158P3D2 MAbs in human bladder cancer xenograft mouse models is evaluated by using recombinant cell lines such as J82-158P3D2 (see, e.g., Kaighn, M. E., et al., Invest Urol, 1979. 17(1): p. 16-23), as well as human bladder xenograft models (SCaBER). 
     Antibody efficacy on tumor growth and metastasis formation is studied, e.g., in a mouse orthotopic bladder cancer xenograft model. The antibodies can be unconjugated, as discussed in this Example, or can be conjugated to a therapeutic modality (see below), as appreciated in the art. Anti-158P3D2 MAbs inhibit formation of bladder xenografts. Anti-158P3D2 MAbs retard the growth of established orthotopic tumors and prolong survival of tumor-bearing mice. MAb effects on tumor growth in mouse models support the utility of anti-158P3D2 MAbs in the treatment of local and advanced stages of bladder cancer (see, e.g., Saffran, D., 2001, et al., PNAS 10:1073-1078). 
     Administration of the anti-158P3D2 MAbs leads to retardation of established orthotopic tumor growth and inhibition of metastasis to distant sites, resulting in a significant prolongation in the survival of tumor-bearing mice. Therefore, 158P3D2 is an attractive target for immunotherapy, and anti-158P3D2 MAbs have therapeutic potential for the treatment of local and metastatic cancer. This example demonstrates that unconjugated 158P3D2 monoclonal antibodies are effective to inhibit the growth of human bladder tumor xenografts grown in SCID mice; accordingly, a combination of such efficacious MAbs is also effective. 
     MAb-Toxin Conjugates: 
     Another embodiment of MAb therapy is through the use of toxin conjugation of MAbs for targeted delivery of cytotoxic agents to cells expressing the protein target. Major advances have been made in the clinical application of MAb toxin conjugates with the development of Mylotarg for acute myeloid leukemia (Bross, P. F., et al., 2001, Clin. Cancer Res. 7:1490-1496). Mylotarg is a humanized MAb directed to CD33 which is conjugated to a highly potent DNA-alkylating agent (calichemicin) via an acid labile hydrazone bond (Hamann, P. R., et al., 2002, Bioconjug. Chem. 13:40-46; ibid., 13:47-58). Additional toxins for MAb conjugation in development include maytansinoid, doxorubicin, taxoids and the potent synthetic dolastatin 10 analogs auristatin E and monomethylauristatin E (Doronina, S. O., et al., 2003, Nature Biotech. 21:778-784; Ross, S., et al., 2002, Cancer Res. 62:2546-2553; Francisco, J. A., et el., 2003, Blood 102 :1458-1465; Mao, W., et al., 2004, Cancer Res. 64:781-788). Such applications have potential to deliver a cytotoxic agent to cells expressing the protein target of the MAb. Internalization of the target protein upon MAb binding is important for toxin delivery, and the mechanism spares the non-targeted tissues from the potentially harmful effects of the cytotoxic agent. 
     158P3D2 MAbs conjugated to toxins are used to induce cell killing in vitro using established protocols for cytotoxicity assays and clonogenic assays (Doronina, S. O., et al., 2003, Nature Biotech. 21:778-784; Mao, W., et al., 2004, Cancer Res. 64:781-788). Toxin conjugated anti-158P3D2 MAbs induce cytotoxicity of cells expressing endogenous 158P3D2 (SCaBER cells) and recombinant 158P3D2 (PC3-158P3D2, 3T3-158P3D2, Rat-1-158P3D2 and B300.19-158P3D2). This methodology allows confirmation that the toxin conjugated MAb is functional against cells expressing the 158P3D2 protein on their surface versus those that do not express the target. 
     The MAb toxin conjugates are tested for their ability to inhibit tumor growth in vivo. Antibody efficacy on tumor growth and metastasis formation is studied, e.g., in a mouse orthotopic bladder cancer xenograft model, a mouse lung cancer xenograft model, or mouse colon or breast cancer xenograft model. Administration of the anti-158P3D2 MAbs led to retardation of established orthotopic tumor growth and inhibition of metastasis to distant sites, resulting in a significant prolongation in the survival of tumor-bearing mice. These studies indicate that 158P3D2 is an attractive target for immunotherapy and demonstrate the therapeutic potential of toxin-conjugated anti-158P3D2 MAbs for the treatment of local and metastatic cancer. This example demonstrates that toxin-conjugated 158P3D2 monoclonal antibodies are effective to inhibit the growth of human bladder, lung, breast and colon tumor xenografts grown in SCID mice; accordingly, a combination of such efficacious MAbs is also effective. The methodology allows the targeted delivery of a cytotoxin using a plasma stable linker in a MAb-toxin conjugate. Such a mechanism of action reduces the potential harmful effects of the toxin on non-targeted tissues. 
     Tumor Inhibition Using Multiple Unconjugated or Toxin-Conjugated 158P3D2 Mabs 
     Materials and Methods 
     158P3D2 Monoclonal Antibodies: 
     Monoclonal antibodies were raised against 158P3D2 as described in the Example entitled “Generation of 158P3D2 Monoclonal Antibodies (MAbs).” The antibodies are characterized by ELISA, Western blot, FACS, and immunoprecipitation for their capacity to bind 158P3D2. Epitope mapping data for the anti-158P3D2 MAbs, as determined by ELISA and Western analysis, recognize epitopes on the 158P3D2 protein Immunohistochemical analysis of bladder cancer tissues and cells with these antibodies is performed. 
     The monoclonal antibodies are purified from ascites or hybridoma tissue culture supernatants by Protein-G Sepharose chromatography, dialyzed against PBS, filter sterilized, and stored at −20° C. Protein determinations are performed by a Bradford assay (Bio-Rad, Hercules, Calif.). A therapeutic monoclonal antibody or a cocktail comprising a mixture of individual monoclonal antibodies is prepared and used for the treatment of mice receiving subcutaneous or orthotopic injections of SCaBER or J82-158P3D2 tumor xenografts. 
     The MAbs to 158P3D2 are conjugated to various different toxins (listed above) using any of a variety of methods described elsewhere in the art (Hamann, P. R., et al., 2002, Bioconjug. Chem. 13:40-46; ibid., 13:47-58; Doronina, S. O., et al., 2003, Nature Biotech. 21:778-784; Ojima, I., et al. 2002, J. Med. Chem. 45:5620-5623; Dubowchik, G. M., et al., 2002, Bioconjug. Chem. 13:855-869; King, H. D., 2002, J. Med. Chem. 45:4336-4343; Ross, S., et al., 2002, Cancer Res. 62:2546-2553; Francisco, J. A., et el., 2003, Blood 102 :1458-1465; Mao, W., et al., 2004, Cancer Res. 64:781-788). 
     Cell Lines 
     The bladder carcinoma cell lines, J82 and SCaBER, as well as the fibroblast line NIH 3T3 (American Type Culture Collection) are maintained in media supplemented with L-glutamine and 10% FBS. J82-158P3D2 and 3T3-158P3D2 cell populations are generated by retroviral gene transfer as described in Hubert, R. S., et al., Proc. Natl. Acad. Sci. USA, 1999, 96(25):14523. 
     Xenograft Mouse Models 
     Subcutaneous (s.c.) tumors are generated by injection of 1×10 6  cancer cells mixed at a 1:1 dilution with Matrigel® (Collaborative Research) in the right flank of male SCID mice. To test antibody efficacy on tumor formation, i.p. antibody injections are started on the same day as tumor-cell injections. As a control, mice are injected with either purified mouse IgG (ICN) or PBS; or a purified monoclonal antibody that recognizes an irrelevant protein not expressed in human cells. Tumor sizes are determined by caliper measurements, and the tumor volume is calculated as: Length×Width×Height. Mice with s.c. tumors greater than 1.5 cm in diameter are sacrificed. 
     Orthotopic injections are performed under anesthesia by using ketamine/xylazine. For bladder orthotopic studies, an incision is made through the abdomen to expose the bladder, and tumor cells (5×10 5 ) mixed with Matrigel® are injected into the bladder wall in a 10-μl volume. To monitor tumor growth, mice are palpated and blood is collected on a weekly basis to measure BTA levels. For prostate orthopotic models, an incision is made through the abdominal muscles to expose the bladder and seminal vesicles, which then are delivered through the incision to expose the dorsal prostate. Tumor cells, e.g. SCaBER cells (5×10 5 ) mixed with Matrigel® are injected into the bladder in a 10-μl volume (Yoshida Y et al, Anticancer Res. 1998, 18:327; Ahn et al, Tumor Biol. 2001, 22:146). The mice are segregated into groups for the appropriate treatments, with anti-158P3D2 or control MAbs being injected i.p. 
     Anti-158P3D2 MAbs Inhibit Growth of 158P3D2-Expressing Xenograft-Cancer Tumors 
     The effect of anti-158P3D2 MAbs on tumor formation is tested on the growth and progression of bladder cancer xenografts using SCaBER and J82-158P3D2 orthotopic models. As compared with the s.c. tumor model, the orthotopic model, which requires injection of tumor cells directly in the mouse bladder, and prostate, respectively, results in a local tumor growth, development of metastasis in distal sites, deterioration of mouse health, and subsequent death (Saffran, D., et al., PNAS supra; Fu, X., et al., Int J Cancer, 1992. 52(6): p. 987-90; Kubota, T., J Cell Biochem., 1994. 56(1): p. 4-8). The features make the orthotopic model more representative of human disease progression and allowed us to follow the therapeutic effect of MAbs on clinically relevant end points. 
     Accordingly, tumor cells are injected into the mouse bladder, or lung, and 2 days later, the mice are segregated into two groups and treated with either: a) 200-500 μg of anti-158P3D2 MAb, or b) PBS three times per week for two to five weeks. 
     A major advantage of the orthotopic cancer models is the ability to study the development of metastases. Formation of metastasis in mice bearing established orthotopic tumors is studies by IHC analysis on lung sections using an antibody against a tumor-specific cell-surface protein such as anti-cytokeratin 20 for bladder cancer models (Lin S et al, Cancer Detect Prey. 2001; 25:202). 
     Mice bearing established orthotopic tumors are administered 1000 μg injections of either anti-158P3D2 MAb or PBS over a 4-week period. Mice in both groups are allowed to establish a high tumor burden, to ensure a high frequency of metastasis formation in mouse lungs. Mice then are killed and their bladders, livers, bone and lungs are analyzed for the presence of tumor cells by IHC analysis. 
     Anti-158P3D2 antibodies inhibit the formation of tumors, retard the growth of already established tumors, and prolong the survival of treated mice. Moreover, anti-158P3D2 MAbs demonstrate a dramatic inhibitory effect on the spread of local bladder tumors to distal sites, even in the presence of a large tumor burden. Thus, anti-158P3D2 MAbs are efficacious on major clinically relevant end points (tumor growth), prolongation of survival, and health. 
     Example 39 
     Therapeutic and Diagnostic Use of Anti-158P3D2 Antibodies in Humans 
     Anti-158P3D2 monoclonal antibodies are safely and effectively used for diagnostic, prophylactic, prognostic and/or therapeutic purposes in humans. Western blot and immunohistochemical analysis of cancer tissues and cancer xenografts with anti-158P3D2 mAb show strong extensive staining in carcinoma but significantly lower or undetectable levels in normal tissues. Detection of 158P3D2 in carcinoma and in metastatic disease demonstrates the usefulness of the mAb as a diagnostic and/or prognostic indicator. Anti-158P3D2 antibodies are therefore used in diagnostic applications such as immunohistochemistry of kidney biopsy specimens to detect cancer from suspect patients. 
     As determined by flow cytometry, anti-158P3D2 mAb specifically binds to carcinoma cells. Thus, anti-158P3D2 antibodies are used in diagnostic whole body imaging applications, such as radioimmunoscintigraphy and radioimmunotherapy, (see, e.g., Potamianos S., et. al. Anticancer Res 20(2A):925-948 (2000)) for the detection of localized and metastatic cancers that exhibit expression of 158P3D2. Shedding or release of an extracellular domain of 158P3D2 into the extracellular milieu, such as that seen for alkaline phosphodiesterase B10 (Meerson, N. R., Hepatology 27:563-568 (1998)), allows diagnostic detection of 158P3D2 by anti-158P3D2 antibodies in serum and/or urine samples from suspect patients. 
     Anti-158P3D2 antibodies that specifically bind 158P3D2 are used in therapeutic applications for the treatment of cancers that express 158P3D2. Anti-158P3D2 antibodies are used as an unconjugated modality and as conjugated form in which the antibodies are attached to one of various therapeutic or imaging modalities well known in the art, such as a prodrugs, enzymes or radioisotopes. In preclinical studies, unconjugated and conjugated anti-158P3D2 antibodies are tested for efficacy of tumor prevention and growth inhibition in the SCID mouse cancer xenograft models, e.g., kidney cancer models AGS-K3 and AGS-K6, (see, e.g., the Example entitled “158P3D2 Monoclonal Antibody-mediated Inhibition of Bladder and Lung Tumors In Vivo”). Either conjugated and unconjugated anti-158P3D2 antibodies are used as a therapeutic modality in human clinical trials either alone or in combination with other treatments as described in following Examples. 
     Example 40 
     Human Clinical Trials for the Treatment and Diagnosis of Human Carcinomas Through Use of Human Anti-158P3D2 Antibodies In Vivo 
     Antibodies are used in accordance with the present invention which recognize an epitope on 158P3D2, and are used in the treatment of certain tumors such as those listed in Table I. Based upon a number of factors, including 158P3D2 expression levels, tumors such as those listed in Table I are presently preferred indications. In connection with each of these indications, three clinical approaches are successfully pursued. 
     Adjunctive Therapy: 
     In adjunctive therapy, patients are treated with anti-158P3D2 antibodies in combination with a chemotherapeutic or antineoplastic agent and/or radiation therapy. Primary cancer targets, such as those listed in Table I, are treated under standard protocols by the addition anti-158P3D2 antibodies to standard first and second line therapy. Protocol designs address effectiveness as assessed by reduction in tumor mass as well as the ability to reduce usual doses of standard chemotherapy. These dosage reductions allow additional and/or prolonged therapy by reducing dose-related toxicity of the chemotherapeutic agent. Anti-158P3D2 antibodies are utilized in several adjunctive clinical trials in combination with the chemotherapeutic or antineoplastic agents adriamycin (advanced prostrate carcinoma), cisplatin (advanced head and neck and lung carcinomas), taxol (breast cancer), and doxorubicin (preclinical). 
     Monotherapy: 
     In connection with the use of the anti-158P3D2 antibodies in monotherapy of tumors, the antibodies are administered to patients without a chemotherapeutic or antineoplastic agent. In one embodiment, monotherapy is conducted clinically in end stage cancer patients with extensive metastatic disease. Patients show some disease stabilization. Trials demonstrate an effect in refractory patients with cancerous tumors. 
     Imaging Agent: 
     Through binding a radionuclide (e.g., iodine or yttrium (I 131 , Y 90 ) to anti-158P3D2 antibodies, the radiolabeled antibodies are utilized as a diagnostic and/or imaging agent. In such a role, the labeled antibodies localize to both solid tumors, as well as, metastatic lesions of cells expressing 158P3D2. In connection with the use of the anti-158P3D2 antibodies as imaging agents, the antibodies are used as an adjunct to surgical treatment of solid tumors, as both a pre-surgical screen as well as a post-operative follow-up to determine what tumor remains and/or returns. In one embodiment, a ( 111 In)-158P3D2 antibody is used as an imaging agent in a Phase I human clinical trial in patients having a carcinoma that expresses 158P3D2 (by analogy see, e.g., Divgi et al.  J. Natl. Cancer Inst.  83:97-104 (1991)). Patients are followed with standard anterior and posterior gamma camera. The results indicate that primary lesions and metastatic lesions are identified. 
     Dose and Route of Administration 
     As appreciated by those of ordinary skill in the art, dosing considerations can be determined through comparison with the analogous products that are in the clinic. Thus, anti-158P3D2 antibodies can be administered with doses in the range of 5 to 400 mg/m 2 , with the lower doses used, e.g., in connection with safety studies. The affinity of anti-158P3D2 antibodies relative to the affinity of a known antibody for its target is one parameter used by those of skill in the art for determining analogous dose regimens. Further, anti-158P3D2 antibodies that are fully human antibodies, as compared to the chimeric antibody, have slower clearance; accordingly, dosing in patients with such fully human anti-158P3D2 antibodies can be lower, perhaps in the range of 50 to 300 mg/m 2 , and still remain efficacious. Dosing in mg/m 2 , as opposed to the conventional measurement of dose in mg/kg, is a measurement based on surface area and is a convenient dosing measurement that is designed to include patients of all sizes from infants to adults. 
     Three distinct delivery approaches are useful for delivery of anti-158P3D2 antibodies. Conventional intravenous delivery is one standard delivery technique for many tumors. However, in connection with tumors in the peritoneal cavity, such as tumors of the ovaries, biliary duct, other ducts, and the like, intraperitoneal administration may prove favorable for obtaining high dose of antibody at the tumor and to also minimize antibody clearance. In a similar manner, certain solid tumors possess vasculature that is appropriate for regional perfusion. Regional perfusion allows for a high dose of antibody at the site of a tumor and minimizes short term clearance of the antibody. 
     Clinical Development Plan (CDP) 
     Overview: The CDP follows and develops treatments of anti-158P3D2 antibodies in connection with adjunctive therapy, monotherapy, and as an imaging agent. Trials initially demonstrate safety and thereafter confirm efficacy in repeat doses. Trails are open label comparing standard chemotherapy with standard therapy plus anti-158P3D2 antibodies. As will be appreciated, one criteria that can be utilized in connection with enrollment of patients is 158P3D2 expression levels in their tumors as determined by biopsy. 
     As with any protein or antibody infusion-based therapeutic, safety concerns are related primarily to (i) cytokine release syndrome, i.e., hypotension, fever, shaking, chills; (ii) the development of an immunogenic response to the material (i.e., development of human antibodies by the patient to the antibody therapeutic, or HAHA response); and, (iii) toxicity to normal cells that express 158P3D2. Standard tests and follow-up are utilized to monitor each of these safety concerns. Anti-158P3D2 antibodies are found to be safe upon human administration. 
     Example 41 
     Human Clinical Trial Adjunctive Therapy with Human Anti-158P3D2 Antibody and Chemotherapeutic Agent 
     A phase I human clinical trial is initiated to assess the safety of six intravenous doses of a human anti-158P3D2 antibody in connection with the treatment of a solid tumor, e.g., a cancer of a tissue listed in Table I. In the study, the safety of single doses of anti-158P3D2 antibodies when utilized as an adjunctive therapy to an antineoplastic or chemotherapeutic agent as defined herein, such as, without limitation: cisplatin, topotecan, doxorubicin, adriamycin, taxol, or the like, is assessed. The trial design includes delivery of six single doses of an anti-158P3D2 antibody with dosage of antibody escalating from approximately about 25 mg/m 2  to about 275 mg/m 2  over the course of the treatment in accordance with the following schedule: 
     
       
         
           
               
               
               
               
               
               
               
             
               
                   
                   
               
               
                   
                 Day 0 
                 Day 7 
                 Day 14 
                 Day 21 
                 Day 28 
                 Day 35 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 mAb Dose 
                 25 
                 75 
                 125 
                 175 
                 225 
                 275 
               
               
                   
                 mg/m 2   
                 mg/m 2   
                 mg/m 2   
                 mg/m 2   
                 mg/m 2   
                 mg/m 2   
               
               
                 Chemotherapy 
                 + 
                 + 
                 + 
                 + 
                 + 
                 + 
               
               
                 (standard dose) 
               
               
                   
               
            
           
         
       
     
     Patients are closely followed for one-week following each administration of antibody and chemotherapy. In particular, patients are assessed for the safety concerns mentioned above: (i) cytokine release syndrome, i.e., hypotension, fever, shaking, chills; (ii) the development of an immunogenic response to the material (i.e., development of human antibodies by the patient to the human antibody therapeutic, or HAHA response); and, (iii) toxicity to normal cells that express 158P3D2. Standard tests and follow-up are utilized to monitor each of these safety concerns. Patients are also assessed for clinical outcome, and particularly reduction in tumor mass as evidenced by MRI or other imaging. 
     The anti-158P3D2 antibodies are demonstrated to be safe and efficacious, Phase II trials confirm the efficacy and refine optimum dosing. 
     Example 42 
     Human Clinical Trial 
     Monotherapy with Human Anti-158P3D2 Antibody 
     Anti-158P3D2 antibodies are safe in connection with the above-discussed adjunctive trial, a Phase II human clinical trial confirms the efficacy and optimum dosing for monotherapy. Such trial is accomplished, and entails the same safety and outcome analyses, to the above-described adjunctive trial with the exception being that patients do not receive chemotherapy concurrently with the receipt of doses of anti-158P3D2 antibodies. 
     Example 43 
     Human Clinical Trial 
     Diagnostic Imaging with Anti-158P3D2 Antibody 
     Once again, as the adjunctive therapy discussed above is safe within the safety criteria discussed above, a human clinical trial is conducted concerning the use of anti-158P3D2 antibodies as a diagnostic imaging agent. The protocol is designed in a substantially similar manner to those described in the art, such as in Divgi et al.  J. Natl. Cancer Inst.  83:97-104 (1991). The antibodies are found to be both safe and efficacious when used as a diagnostic modality. 
     Example 44 
     158P3D2 Functional Assays 
     158P3D2 protein, and variants thereof, is a member of a family of related proteins, the ferlins. This family of membrane proteins is characterized by the presence of intracellular C2 domains, so named by their homology to a conserved protein kinase C (PKC) motif. The canonical C2 domain is a 130 amino acid long Ca 2+  dependent membrane targeting module that is found in proteins involved in signal transduction or membrane trafficking (Rizo, J. and Sudhof, T. C., J. Biol. Chem. 273, 15879-82 (1998)). The function of the C2 domain amongst the &gt;100 proteins identified to date varies between these proteins, however a common feature is that the C2 domain has been shown to bind to phospholipids, particularly phosphatidylserine and phosphatidylcholine. In some cases, the C2 domain may not bind to Ca 2+  or to phospholipids but rather to other proteins (Rizo, J. and Sudhof, T. C., J. Biol. Chem. 273, 15879-82 (1998)). 158P3D2, and variants thereof, are Ca 2+  binding proteins with the capacity to bind to both phospholipids and to proteins. The different variants of 158P3D2, which express different numbers of C2 domains, have different functions with respect to the unique combinations of expressed C2 regions. 
     Dysferlin is a member of this family of C2 containing proteins that has a function in muscle membrane repair. Human mutation of dysferlin leads to specific autosomal recessive muscular dystrophies (limb-girdle MD type 2B and Miyoshi myopathy) (reviewed in Bansal, D. and Campbell, K. P., Trends in Cell Biol. 14, 206-213). Dysferlin is localized in the plasma membrane of cells where it interacts with annexin A1 and A2, and is also found in vesicles. Membrane disruption (for example in muscle) causes an increase of localized Ca 2+  at the wound site and an accumulation of vesicles containing dysferlin. The dysferlin protein facilitates both docking and fusion of the vesicles with the plasma membrane through interaction with the annexins and/or other membrane-associated proteins. Fusion between the repair vesicles and the plasma membrane seals the wound (Bansal, D. and Campbell, K. P., Trends in Cell Biol. 14, 206-213). 
     158P3D2 protein, and variants thereof, functions in a similar fashion as dysferlin by inducing repair of cellular plasma membranes following their disruption. Given the high rate of cell division and stress conditions such as hypoxia and reduced nutrient supply during tumor formation, membrane repair becomes a critical component of tumor survival. Expression of 158P3D2, and variants thereof, on tumors provides an advantage for such cells to grow under stressful conditions such as hypoxia or nutrient deprivation. 
     The C2 domain of the lipid phosphatase/tumor suppressor PTEN is regulated by threonine phosphorylation (Raftopolou, M., et al., 2004, Science, 303, 1179-81). This phosphorylation event inhibits cell migration independent of the lipid phosphatase activity, which may relate to the tumor suppressive activity of PTEN. However, given the regulation of C2-induced function by phosphorylation, the status of that phosphorylation event alters the migratory capacity of the cell. 158P3D2 protein, and variants thereof, reside in the plasma membrane of tumor cells as C2-containing regulators of cell migration due to alterations in the phosphorylation status of 158P3D2. Upon phosphorylation of 158P3D2, the C2 domains influence the migratory capacity of 158P3D2-positive tumor cells, conferring an advantage for them to migrate to distal sites to seek secondary growth (metastasis). 158P3D2, and variants thereof, also bind to signal transduction proteins, providing important signaling cascades for tumor cells that confer a growth advantage and increased capacity for cell migration and adhesion. Such advantages are key elements for increased survival and metastasis for bladder, lung, colon and breast cancer cells. 
     Enhanced proliferation and entry into S-phase of tumor cells relative to normal cells is a hallmark of the cancer cell phenotype. To address the effect of expression of 158P3D2 on the proliferation rate of normal cells, two rodent cell lines (3T3 and Rat-1) are infected with virus containing the 158P3D2 gene and stable cells expressing 158P3D2 antigen are derived, as well as empty vector control cells expressing the selection marker neomycin (Neo). The cells are grown overnight in 0.5% FBS and then compared to cells treated with 10% FBS. The cells are evaluated for proliferation at 18-96 hr post-treatment by a  3 H-thymidine incorporation assay and for cell cycle analysis by a BrdU incorporation/propidium iodide staining assay. Rat-1 cells expressing the 158P3D2 antigen grow effectively in low serum concentrations (0.1%) compared to the Rat-1-Neo cells. Similar data are obtained for the 3T3 cells expressing 158P3D2 versus Neo only. To assess cell proliferation by another methodology, the cells are stained with BrdU and propidium iodide. Briefly, cells are labeled with 10 μM BrdU, washed, trypsinized and fixed in 0.4% paraformaldehyde and 70% ethanol. Anti-BrdU-FITC (Pharmigen) is added to the cells, the cells are washed and then incubated with 10 μg/ml propidium iodide for 20 min prior to washing and analysis for fluorescence at 488 nm. An increase in labeling of cells in S-phase (DNA synthesis phase of the cell cycle) in 3T3 cells that express the 158P3D2 protein is observed relative to control cells. This confirms the results of those measured by  3 H-thymidine incorporation. Accordingly, 158P3D2 expressing cells have increased potential for growth as tumor cells in vivo during stress, including nutrient deprivation, hypoxia or reduced osmolarity. 
     Example 45 
     158P3D2 RNA Interference (RNAi) 
     RNA interference (RNAi) technology is implemented to a variety of cell assays relevant to oncology. RNAi is a post-transcriptional gene silencing mechanism activated by double-stranded RNA (dsRNA). RNAi induces specific mRNA degradation leading to changes in protein expression and subsequently in gene function. In mammalian cells, these dsRNAs called short interfering RNA (siRNA) have the correct composition to activate the RNAi pathway targeting for degradation, specifically some mRNAs. See, Elbashir S. M., et al.,  Duplexes of  21- nucleotide RNAs Mediate RNA interference in Cultured Mammalian Cells , Nature 411(6836):494-8 (2001). Thus, RNAi technology is used successfully in mammalian cells to silence targeted genes. 
     Loss of cell proliferation control is a hallmark of cancerous cells; thus, assessing the role of 158P3D2 in cell survival/proliferation assays is relevant. Accordingly, RNAi was used to investigate the function of the 158P3D2 antigen. To generate siRNA for 158P3D2, algorithms were used that predict oligonucleotides that exhibit the critical molecular parameters (G:C content, melting temperature, etc.) and have the ability to significantly reduce the expression levels of the 158P3D2 protein when introduced into cells. Accordingly, one targeted sequence for the 158P3D2 siRNA is: 5′ CCTCGGCAGCCAATCAGCTAT 3′ (SEQ ID NO:104)(oligo 158P3D2.b). In accordance with this Example, 158P3D2 siRNA compositions are used that comprise siRNA (double stranded, short interfering RNA) that correspond to the nucleic acid ORF sequence of the 158P3D2 protein or subsequences thereof. Thus, siRNA subsequences are used in this manner are generally 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35 or more than 35 contiguous RNA nucleotides in length. These siRNA sequences are complementary and non-complementary to at least a portion of the mRNA coding sequence. In a preferred embodiment, the subsequences are 19-25 nucleotides in length, most preferably 21-23 nucleotides in length. In preferred embodiments, these siRNA achieve knockdown of 158P3D2 antigen in cells expressing the protein and have functional effects as described below. 
     The selected siRNA (158P3D2.b oligo) was tested in numerous cell lines in the thymidine incorporation/proliferation assay (measures  3 H-Thy uptake and incorporation into DNA). Moreover, this 158P3D2.b oligo achieved knockdown of 158P3D2 antigen in cells expressing the protein and had functional effects as described below using the following protocols. 
     Mammalian siRNA Transfections: 
     The day before siRNA transfection, the different cell lines were plated in media (RPMI 1640 with 10% FBS w/o antibiotics) at 2×10 3  cells/well in 80 μl (96 well plate format) for the survival/MTS assay. In parallel with the 158P3D2 specific siRNA oligo, the following sequences were included in every experiment as controls: a) Mock transfected cells with Lipofectamine 2000 (Invitrogen, Carlsbad, Calif.) and annealing buffer (no siRNA); b) Luciferase-4 specific siRNA (targeted sequence: 5′-AAGGGACGAAGACGAACACUUCTT-3′) (SEQ ID NO:105); and, c) Eg5 specific siRNA (targeted sequence: 5′-AACTGAAGACCTGAAGACAATAA-3′) (SEQ ID NO:106). SiRNAs were used at 10 nM and 1 μg/ml Lipofectamine 2000 final concentration. 
     The procedure was as follows: The siRNAs were first diluted in OPTIMEM (serum-free transfection media, Invitrogen) at 0.1 uM μM (10-fold concentrated) and incubated 5-10 min RT. Lipofectamine 2000 was diluted at 10 μg/ml (10-fold concentrated) for the total number transfections and incubated 5-10 minutes at room temperature (RT). Appropriate amounts of diluted 10-fold concentrated Lipofectamine 2000 were mixed 1:1 with diluted 10-fold concentrated siRNA and incubated at RT for 20-30″ (5-fold concentrated transfection solution). 20 μls of the 5-fold concentrated transfection solutions were added to the respective samples and incubated at 37° C. for 96 hours before analysis. 
       3 H-Thymidine Incorporation Assay: 
     The proliferation assay is a  3 H-thymidine incorporation method for determining the proliferation of viable cells by uptake and incorporation of label into DNA. 
     The procedure was as follows: Cells growing in log phase are trypsinized, washed, counted and plated in 96-well plates at 1000-4000 cells/well in 10% FBS. After 4-8 hrs, the media is replaced. The cells are incubated for 24-72 hrs, pulsed with  3 H-Thy at 1.5 μCi/ml for 14 hrs, harvested onto a filtermat and counted in scintillation cocktail on a Microbeta trilux or other counter. 
     To address the validation of the 158P3D2 siRNA in reducing the expression of 158P3D2 protein in cells, Cos-1 cells were transfected with pcDNA.3 vector expressing a Myc/His-tagged version of 158P3D2 alone (LF2k) or together with siRNA for either CT1 (bacterial negative control) or 158P3D2 oligo (158P3D2.b). For additional control, a mock transfection was also included (No DNA). Western blot analysis using an antibody to the Myc tag on 158P3D2 protein showed that the 158P3D2 siRNA significantly reduced the expression level of 158P3D2 protein ( FIG. 30 ). These data show that the specific 158P3D2.b siRNA will have utility to probe the function of 158P3D2 protein in cells. 
     In order to address the function of 158P3D2 in cells, 158P3D2 was silenced by transfecting the endogenously expressing 158P3D2 cell lines (SCaBER, a bladder cancer cell line) with the 158P3D2 specific siRNA (158P3D2.b) along with negative siRNA controls (Luc4, targeted sequence not represented in the human genome) and a positive siRNA control (targeting Eg5) (See  FIG. 4 ). SCaBER cells were shown to express 158P3D2 by Northern blot of total cellular RNA. The results indicated that when these cells were treated with siRNA specifically targeting the 158P3D2 mRNA, the resulting “158P3D2 deficient cells” showed diminished cell proliferation as measured by this assay (see oligo 158P3D2.b treated cells). This effect is likely caused by an active induction of apoptosis. The reduced viability is measured by the decreased uptake of labeled thymidine. 
     As control, Cos-1 cells, a cell line with no detectable expression of 158P3D2 mRNA or protein (by Western blot), was also treated with the panel of siRNAs (including oligo 158P3D2.b) and no phenotype was observed ( FIG. 4 ). This result reflects the fact that the specific protein knockdown in the SCaBER cells is not a function of general toxicity, since the Cos-1 cells did not respond to the 158P3D2.b oligo. The differential response of the two cell lines to the Eg5 control is a reflection of differences in levels of cell transfection and responsiveness of the cell lines to oligo treatment ( FIG. 4 ). 
     Together, these data indicate that 158P3D2, and variants thereof, play important roles in the proliferation of cancer cells and that the lack of 158P3D2 clearly decreases the survival potential of these cells. It is to be noted that 158P3D2 is constitutively expressed in many tumor cell lines. 158P3D2 serves a role in malignancy; it expression is a primary indicator of disease, where such disease is often characterized by high rates of uncontrolled cell proliferation and diminished apoptosis. Correlating cellular phenotype with gene knockdown following RNAi treatments is important, and allows one to draw valid conclusions and rule out toxicity or other non-specific effects of these reagents. To this end, assays to measure the levels of expression of both protein and mRNA for the target after RNAi treatments are important, including Western blotting, FACS staining with antibody, immunoprecipitation, Northern blotting or RT-PCR (Taqman or standard methods). Any phenotypic effect of the siRNAs in these assays should be correlated with the protein and/or mRNA knockdown levels in the same cell lines. Knockdown of 158P3D2 is achieved using the 158P3D2.b oligo as measured by Western blotting and RT-PCR analysis. 
     Another method to analyze 158P3D2 related cell proliferation is performing clonogenic assays. In these assays, a defined number of cells are plated onto the appropriate matrix and the number of colonies formed after a period of growth following siRNA treatment is counted. 
     In 158P3D2 cancer target validation, complementing the cell survival/proliferation analysis with apoptosis and cell cycle profiling studies are considered. The biochemical hallmark of the apoptotic process is genomic DNA fragmentation, an irreversible event that commits the cell to die. A method to observe fragmented DNA in cells is the immunological detection of histone-complexed DNA fragments by an immunoassay (i.e. cell death detection ELISA) which measures the enrichment of histone-complexed DNA fragments (mono- and oligo-nucleosomes) in the cytoplasm of apoptotic cells. This assay does not require pre-labeling of the cells and can detect DNA degradation in cells that do not proliferate in vitro (i.e. freshly isolated tumor cells). 
     The most important effector molecules for triggering apoptotic cell death are caspases. Caspases are proteases that when activated cleave numerous substrates at the carboxy-terminal site of an aspartate residue mediating very early stages of apoptosis upon activation. All caspases are synthesized as pro-enzymes and activation involves cleavage at aspartate residues. In particular, caspase 3 seems to play a central role in the initiation of cellular events of apoptosis. Assays for determination of caspase 3 activation detect early events of apoptosis. Following RNAi treatments, Western blot detection of active caspase 3 presence or proteolytic cleavage of products (i.e. PARP) found in apoptotic cells further support an active induction of apoptosis. Because the cellular mechanisms that result in apoptosis are complex, each has its advantages and limitations. Consideration of other criteria/endpoints such as cellular morphology, chromatin condensation, membrane blebbing, apoptotic bodies help to further support cell death as apoptotic. Since not all the gene targets that regulate cell growth are anti-apoptotic, the DNA content of permeabilized cells is measured to obtain the profile of DNA content or cell cycle profile. Nuclei of apoptotic cells contain less DNA due to the leaking out to the cytoplasm (sub-G1 population). In addition, the use of DNA stains (i.e., propidium iodide) also differentiates between the different phases of the cell cycle in the cell population due to the presence of different quantities of DNA in G0/G1, S and G2/M. In these studies the subpopulations can be quantified. 
     For the 158P3D2 gene, RNAi studies facilitate the understanding of the contribution of the gene product in cancer pathways. Such active RNAi molecules have use in identifying assays to screen for mAbs that are active anti-tumor therapeutics. Further, siRNA are administered as therapeutics to cancer patients for reducing the malignant growth of several cancer types, including those listed in Table 1. When 158P3D2 (and variants) plays a role in cell survival, cell proliferation, tumorigenesis, or apoptosis, it is used as a target for diagnostic, prognostic, preventative and/or therapeutic purposes. 
     Example 46 
     Homology Comparison of 158P3D2 to Known Sequences 
     The 158P3D2 v.17 protein has 2036 amino acids with a calculated molecular weight of 227.6 kDa and a pI of 5.64. 158P3D2 is predicted to be a predominantly cytoplasmic protein with plasma membrane association (PSORT-II). 158P3D2 contains a single transmembrane region from amino acids 2000-2022 with high probability that the amino-terminus resides outside, consistent with the topology of a type I transmembrane protein. Based on the TMpred algorithm of Hofmann and Stoffel which utilizes TMBASE (K. Hofmann, W. Stoffel, TMBASE—A database of membrane spanning protein segments Biol. Chem. Hoppe-Seyler 374:166, 1993), 158P3D2 contains a primary transmembrane region from amino acids 2003-2020 (contiguous amino acids with values greater than 0 on the plot have high probability of being transmembrane regions) with an orientation in which the amino terminus resides inside and the carboxyl terminus outside (type II). Another transmembrane algorithm indicated that 158P3D2 contains a transmembrane domain from amino acids 2003-2022, with the N-terminus oriented intracellularly consistent with a type II topology. The transmembrane prediction algorithms are accessed through the ExPasy molecular biology server. 
     By use of the PubMed website of the N.C.B.I., it was found at the protein level that 158P3D2 v.17 shows 60% homology and 40% identity with human otoferlin, a member of the ferlin family of plasma membrane proteins. Further, 158P3D2 v.17 shows 50% homology and 30% identity with dysferlin, another member of the ferlin family, and 80% homology and 75% identity with the murine gene Fer-1-like 4. 
     The ferlins are a family of transmembrane proteins that have function in membrane trafficking, including the repair of cell membranes. Mutation of human otoferlin leads to a specific form of nonsyndromic autosomal recessive deafness (DFNB9) and mutations in dysferlin lead to two subtypes of muscular dystrophies (reviewed in Bansal, D. and Campbell, K. P., Trends in Cell Biol. 14, 206-213). The major feature of the ferlin family includes multiple C2 domains (conserved PKC homologous region) that function in both Ca 2+  dependent and Ca 2+  independent phospholipid binding, as well as protein binding. The mechanism of action for dysferlin includes the repair of muscle cell membrane disruptions through dysferlin-containing cell vesicles. Such vesicles are tethered to the site of membrane tears (where Ca 2+  concentrations are increased) via dysferlin molecules that interact with plasma membrane associated annexin A1 and annexin A2 molecules. The vesicles provide the lipid bilayer material to seal the wound. 
     158P3D2 associates with cell vesicles and the plasma membrane thereby providing a means for tumor cells to repair membranes during tumor growth and metastasis. Such a functional advantage can be exemplified by the increased stress that tumors experience, including increased hypoxia, decreased nutrition and increases in free radical formation. These stresses can alter membrane integrity, thereby increasing the need for robust plasma membrane repair mechanisms. In addition, the C2 domains of 158P3D2, and variants thereof, regulate migration of tumor cells expressing this protein. The regulation of the C2 domains occurs through the phosphorylation of threonine residues (Raftopolou, M., et al., 2004, Science, 303, 1179-81) and modulates the ability of the expressing cells to migrate. This signal transduction property of the 158P3D2 protein expressed in tumor cells enhances their ability to migrate during metastases, facilitates their homing to distal sites (lymph nodes), and promotes interactions with other cells during the formation of tumor masses. Further, the C2 domains of 158P3D2 play a role in membrane trafficking though interaction with Ca 2+ , phosphatidylserine and phosphatidylcholine (Rizo, J. and Sudhof, T. C., J. Biol. Chem. 273, 15879-82 (1998). These interactions are crucial for subsequent membrane metabolism and interaction. Taken together, the 158P3D2 protein significantly promotes unregulated growth of cancer cells, contributing to their viability and metastatic advantage in vivo. 
     The present invention is not to be limited in scope by the embodiments disclosed herein, which are intended as single illustrations of individual aspects of the invention, and any that are functionally equivalent are within the scope of the invention. Various modifications to the models and methods of the invention, in addition to those described herein, will become apparent to those skilled in the art from the foregoing description and teachings, and are similarly intended to fall within the scope of the invention. Such modifications or other embodiments can be practiced without departing from the true scope and spirit of the invention. 
     Tables 
     
       
         
           
               
             
               
                 TABLE I 
               
               
                   
               
               
                 TISSUES THAT EXPRESS 158P3D2 WHEN MALIGNANT. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                   
                 Prostate 
               
               
                   
                 Bladder 
               
               
                   
                 Kidney 
               
               
                   
                 Colon 
               
               
                   
                 Ovary 
               
               
                   
                 Lung 
               
               
                   
                 Breast 
               
               
                   
                 Pancreas 
               
               
                   
                 Stomach 
               
               
                   
                 Cervix 
               
               
                   
                 Lymph node 
               
               
                   
                 Uterus 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE II 
               
             
            
               
                   
               
               
                 AMINO ACID ABBREVIATIONS 
               
            
           
           
               
               
               
               
            
               
                   
                 SINGLE LETTER 
                 THREE LETTER 
                 FULL NAME 
               
               
                   
               
               
                   
                 F 
                 Phe 
                 phenylalanine 
               
               
                   
                 L 
                 Leu 
                 leucine 
               
               
                   
                 S 
                 Ser 
                 serine 
               
               
                   
                 Y 
                 Tyr 
                 tyrosine 
               
               
                   
                 C 
                 Cys 
                 cysteine 
               
               
                   
                 W 
                 Trp 
                 tryptophan 
               
               
                   
                 P 
                 Pro 
                 proline 
               
               
                   
                 H 
                 His 
                 histidine 
               
               
                   
                 Q 
                 Gln 
                 glutamine 
               
               
                   
                 R 
                 Arg 
                 arginine 
               
               
                   
                 I 
                 Ile 
                 isoleucine 
               
               
                   
                 M 
                 Met 
                 methionine 
               
               
                   
                 T 
                 Thr 
                 threonine 
               
               
                   
                 N 
                 Asn 
                 asparagine 
               
               
                   
                 K 
                 Lys 
                 lysine 
               
               
                   
                 V 
                 Val 
                 valine 
               
               
                   
                 A 
                 Ala 
                 alanine 
               
               
                   
                 D 
                 Asp 
                 aspartic acid 
               
               
                   
                 E 
                 Glu 
                 glutamic acid 
               
               
                   
                 G 
                 Gly 
                 glycine 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE III 
               
             
            
               
                   
               
               
                 Amino Acid Substitution Matrix 
               
               
                 Adapted from the GCG Software 9.0 BLOSUM62 amino acid substitution matrix (block 
               
               
                 substitution matrix). The higher the value, the more likely a substitution is found in related, 
               
               
                 natural proteins. (See world wide web URL ikp.unibe.ch/manual/blosum62.html) 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 A 
                 C 
                 D 
                 E 
                 F 
                 G 
                 H 
                 I 
                 K 
                 L 
                 M 
                 N 
                 P 
                 Q 
                 R 
                 S 
                 T 
                 V 
                 W 
                 Y 
                 . 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 4 
                 0 
                 −2 
                 −1 
                 −2 
                 0 
                 −2 
                 −1 
                 −1 
                 −1 
                 −1 
                 −2 
                 −1 
                 −1 
                 −1 
                 1 
                 0 
                 0 
                 −3 
                 −2 
                 A 
               
               
                   
                 9 
                 −3 
                 −4 
                 −2 
                 −3 
                 −3 
                 −1 
                 −3 
                 −1 
                 −1 
                 −3 
                 −3 
                 −3 
                 −3 
                 −1 
                 −1 
                 −1 
                 −2 
                 −2 
                 C 
               
               
                   
                   
                 6 
                 2 
                 −3 
                 −1 
                 −1 
                 −3 
                 −1 
                 −4 
                 −3 
                 1 
                 −1 
                 0 
                 −2 
                 0 
                 −1 
                 −3 
                 −4 
                 −3 
                 D 
               
               
                   
                   
                   
                 5 
                 −3 
                 −2 
                 0 
                 −3 
                 1 
                 −3 
                 −2 
                 0 
                 −1 
                 2 
                 0 
                 0 
                 −1 
                 −2 
                 −3 
                 −2 
                 E 
               
               
                   
                   
                   
                   
                 6 
                 −3 
                 −1 
                 0 
                 −3 
                 0 
                 0 
                 −3 
                 −4 
                 −3 
                 −3 
                 −2 
                 −2 
                 −1 
                 1 
                 3 
                 F 
               
               
                   
                   
                   
                   
                   
                 6 
                 −2 
                 −4 
                 −2 
                 −4 
                 −3 
                 0 
                 −2 
                 −2 
                 −2 
                 0 
                 −2 
                 −3 
                 −2 
                 −3 
                 G 
               
               
                   
                   
                   
                   
                   
                   
                 8 
                 −3 
                 −1 
                 −3 
                 −2 
                 1 
                 −2 
                 0 
                 0 
                 −1 
                 −2 
                 −3 
                 −2 
                 2 
                 H 
               
               
                   
                   
                   
                   
                   
                   
                   
                 4 
                 −3 
                 2 
                 1 
                 −3 
                 −3 
                 −3 
                 −3 
                 −2 
                 −1 
                 3 
                 −3 
                 −1 
                 I 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                 5 
                 −2 
                 −1 
                 0 
                 −1 
                 1 
                 2 
                 0 
                 −1 
                 −2 
                 −3 
                 −2 
                 K 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 4 
                 2 
                 −3 
                 −3 
                 −2 
                 −2 
                 −2 
                 −1 
                 1 
                 −2 
                 −1 
                 L 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 5 
                 −2 
                 −2 
                 0 
                 −1 
                 −1 
                 −1 
                 1 
                 −1 
                 −1 
                 M 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 6 
                 −2 
                 0 
                 0 
                 1 
                 0 
                 −3 
                 −4 
                 −2 
                 N 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 7 
                 −1 
                 −2 
                 −1 
                 −1 
                 −2 
                 −4 
                 −3 
                 P 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 5 
                 1 
                 0 
                 −1 
                 −2 
                 −2 
                 −1 
                 Q 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 5 
                 −1 
                 −1 
                 −3 
                 −3 
                 −2 
                 R 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 4 
                 1 
                 −2 
                 −3 
                 −2 
                 S 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 5 
                 0 
                 −2 
                 −2 
                 T 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 4 
                 −3 
                 −1 
                 V 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 11 
                 2 
                 W 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 7 
                 Y 
               
               
                   
               
            
           
         
       
     
     Table Iv 
     HLA Class I/II Motifs/Supermotifs 
     
       
         
           
               
             
               
                 TABLE IV (A) 
               
             
            
               
                   
               
               
                 HLA Class I Supermotifs/Motifs 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 POSITION 
               
               
                   
                 POSITION 
                 POSITION 
                 C Terminus 
               
               
                   
                 2 (Primary Anchor) 
                 3 (Primary Anchor) 
                 (Primary Anchor) 
               
               
                   
               
               
                 SUPERMOTIF 
                   
                   
                   
               
               
                 A1 
                 
                   TI 
                   LVMS 
                 
                   
                 
                   FWY 
                 
               
               
                   
               
               
                 A2 
                 
                   LIVM 
                   ATQ 
                 
                   
                 
                   IV 
                   MATL 
                 
               
               
                   
               
               
                 A3 
                 
                   VSMA 
                   TLI 
                 
                   
                 
                   RK 
                 
               
               
                   
               
               
                 A24 
                 
                   YF 
                   WIVLMT 
                 
                   
                 
                   FI 
                   YWLM 
                 
               
               
                   
               
               
                 B7 
                 
                   P 
                 
                   
                 
                   VILF 
                   MWYA 
                 
               
               
                   
               
               
                 B27 
                 
                   RHK 
                 
                   
                 
                   FYL 
                   WMIVA 
                 
               
               
                   
               
               
                 B44 
                 
                   E 
                   D 
                 
                   
                 
                   FWYLIMVA 
                 
               
               
                   
               
               
                 B58 
                 
                   ATS 
                 
                   
                 
                   FWY 
                   LIVMA 
                 
               
               
                   
               
               
                 B62 
                 
                   QL 
                   IVMP 
                 
                   
                 
                   FWY 
                   MIVLA 
                 
               
               
                   
               
               
                 MOTIFS 
                   
                   
                   
               
               
                 A1 
                 
                   TSM 
                 
                   
                 
                   Y 
                 
               
               
                   
               
               
                 A1 
                   
                 
                   DE 
                   AS 
                 
                 
                   Y 
                 
               
               
                   
               
               
                 A2.1 
                 
                   LM 
                   VQIAT 
                 
                   
                 
                   V 
                   LIMAT 
                 
               
               
                   
               
               
                 A3 
                 
                   LMVISATF 
                   CGD 
                 
                   
                 
                   KYR 
                   HFA 
                 
               
               
                   
               
               
                 A11 
                 
                   VTMLISAGN 
                   CDF 
                 
                   
                 
                   K 
                   RYH 
                 
               
               
                   
               
               
                 A24 
                   YF W M   
                   
                 
                   FLIW 
                 
               
               
                   
               
               
                 A*3101 
                 
                   MVT 
                   ALIS 
                 
                   
                 
                   R 
                   K 
                 
               
               
                   
               
               
                 A*3301 
                 
                   MVALF 
                   IST 
                 
                   
                 
                   RK 
                 
               
               
                   
               
               
                 A*6801 
                 
                   AVT 
                   MSLI 
                 
                   
                 
                   RK 
                 
               
               
                   
               
               
                 B*0702 
                 
                   P 
                 
                   
                 
                   LMF 
                   WYAIV 
                 
               
               
                   
               
               
                 B*3501 
                 
                   P 
                 
                   
                 
                   LMFWY 
                   IVA 
                 
               
               
                   
               
               
                 B51 
                 
                   P 
                 
                   
                 
                   LIVF 
                   WYAM 
                 
               
               
                   
               
               
                 B*5301 
                 
                   P 
                 
                   
                 
                   IMFWY 
                   ALV 
                 
               
               
                   
               
               
                 B*5401 
                 
                   P 
                 
                   
                 
                   ATIV 
                   LMFWY 
                 
               
               
                   
               
               
                 Bolded residuess are preferred, italicized residues are less preferred: A peptide is considered motif-bearing if it has primary anchors at each primary anchor position for a motif or supermotif as specified in the above table. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IV (B) 
               
             
            
               
                   
               
               
                 HLA Class II Supermotif 
               
            
           
           
               
               
               
            
               
                 1 
                 6 
                 9 
               
               
                   
               
               
                 W, F, Y, V, .I, L 
                 A, V, I, L, P, 
                 A, V, I, L, C, 
               
               
                   
                 C, S, T 
                 S, T, M, Y 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IV (C) 
               
               
                   
               
               
                 HLA Class II Motifs 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                 1° 
                   
                   
                   
                 1° anchor 
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                 MOTIFS 
                   
                 anchor 1 
                 2 3 
                 4 
                 5 
                 6 
                 7 
                 8 
                 9 
               
               
                   
               
               
                 DR4 
                 preferred 
                 FMY LI   
                 M T 
                   
                 I 
                 VST CPA   
                 M 
                   
                 MH 
               
               
                   
                   
                 
                   VW 
                 
                   
                   
                   
                 
                   LIM 
                 
                 H 
                   
                   
               
               
                   
                 deleterious 
                   
                   
                 W 
                   
                   
                 R 
                   
                 W 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 DE 
               
               
                   
               
               
                 DR1 
                 preferred 
                 MF LIV   
                   
                 PAMQ 
                   
                 VMAT S   
                 M 
                   
                 AV 
               
               
                   
                   
                 
                   WY 
                 
                   
                   
                   
                 
                   PLIC 
                 
                   
                   
                 M 
               
               
                   
                 deleterious 
                   
                 C C 
                 FD 
                 CW 
                   
                 GD 
                 D 
                   
               
               
                   
                   
                   
                 H 
                   
                 D 
                   
                 E 
                   
                   
               
               
                   
               
               
                 DR7 
                 preferred 
                 MF LIV   
                 M W 
                 A 
                   
                 IVMSA C   
                 M 
                   
                 IV 
               
               
                   
                   
                 
                   WY 
                 
                   
                   
                   
                 
                   TPL 
                 
                   
                   
                   
               
               
                   
                 deleterious 
                   
                 C 
                 G 
                   
                   
                 GR 
                 N 
                 G 
               
               
                   
                   
                   
                   
                   
                   
                   
                 D 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                   
                 1° 
                   
                   
                 1° 
                   
                 1° anchor 
               
               
                 DR3 
                 MOTIFS 
                 anchor 1 
                 2 
                 3 
                 anchor 4 
                 5 
                 6 
               
               
                   
               
               
                 Motif a 
                   
                 LIVMF 
                   
                   
                 D 
                   
                   
               
               
                 preferred 
                   
                 Y 
                   
                   
                   
                   
                   
               
               
                   
               
               
                 Motif b 
                   
                 LIVMF 
                   
                   
                 DNQES 
                   
                 KRH 
               
               
                 preferred 
                   
                 AY 
                   
                   
                 T 
                   
                   
               
               
                   
               
               
                 DR 
                   
                 MF LIV   
                   
                   
                   
                   
                 VMSTA 
               
               
                 Supermotif 
                   
                 
                   WY 
                 
                   
                   
                   
                   
                 
                   CPLI 
                 
               
               
                   
               
               
                 Italicized residues indicate less preferred or “tolerated” residues 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IV (D) 
               
             
            
               
                   
               
               
                 HLA Class I Supermotifs 
               
            
           
           
               
               
            
               
                 SUPER- 
                 POSITION: 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 MOTIFS 
                   
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
                 7 
                 8 
                 C-terminus 
               
               
                   
               
               
                 A1 
                   
                   
                 
                   1° Anchor 
                 
                   
                   
                   
                   
                   
                   
                 
                   1° Anchor 
                 
               
               
                   
                   
                   
                 TI LVMS   
                   
                   
                   
                   
                   
                   
                 FWY 
               
               
                   
               
               
                 A2 
                   
                   
                 
                   1° Anchor 
                 
                   
                   
                   
                   
                   
                   
                 
                   1° Anchor 
                 
               
               
                   
                   
                   
                 LIVM ATQ   
                   
                   
                   
                   
                   
                   
                 LIVMAT 
               
               
                   
               
               
                 A3 
                 Preferred 
                   
                 
                   1° Anchor 
                 
                 YFW 
                   
                   
                 YFW 
                 YFW 
                 P 
                 
                   1° Anchor 
                 
               
               
                   
                   
                   
                 VSMA TLI   
                 (4/5) 
                   
                   
                 (3/5) 
                 (4/5) 
                 (4/5) 
                 RK 
               
               
                   
                 deleterious 
                 DE (3/5); 
                   
                 DE 
                   
                   
                   
                   
                   
                   
               
               
                   
                   
                 P (5/5) 
                   
                 (4/5) 
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A24 
                   
                   
                 
                   1° Anchor 
                 
                   
                   
                   
                   
                   
                   
                 
                   1° Anchor 
                 
               
               
                   
                   
                   
                 YF WIVLMT   
                   
                   
                   
                   
                   
                   
                 FIY WLM   
               
               
                   
               
               
                 B7 
                 Preferred 
                 FWY (5/5) 
                 
                   1° Anchor 
                 
                 FWY 
                   
                   
                   
                   
                 FWY 
                 
                   1° Anchor 
                 
               
               
                   
                   
                 LIVM 
                 P 
                 (4/5) 
                   
                   
                   
                   
                 (3/5) 
                 VILF MWYA   
               
               
                   
                   
                 (3/5) 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 deleterious 
                 DE (3/5); 
                   
                   
                   
                 DE 
                 G 
                 QN 
                 DE 
                   
               
               
                   
                   
                 P(5/5); 
                   
                   
                   
                 (3/5) 
                 (4/5) 
                 (4/5) 
                 (4/5) 
                   
               
               
                   
                   
                 G(4/5); 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                   
                 A(3/5); 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                   
                 QN(3/5) 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 B27 
                   
                   
                 
                   1° Anchor 
                 
                   
                   
                   
                   
                   
                   
                 
                   1° Anchor 
                 
               
               
                   
                   
                   
                 RHK 
                   
                   
                   
                   
                   
                   
                 FYL WMIVA   
               
               
                   
               
               
                 B44 
                   
                   
                 
                   1° Anchor 
                 
                   
                   
                   
                   
                   
                   
                 
                   1° Anchor 
                 
               
               
                   
                   
                   
                 E D   
                   
                   
                   
                   
                   
                   
                 FWYLIMVA 
               
               
                   
               
               
                 B58 
                   
                   
                 
                   1° Anchor 
                 
                   
                   
                   
                   
                   
                   
                 
                   1° Anchor 
                 
               
               
                   
                   
                   
                 ATS 
                   
                   
                   
                   
                   
                   
                 FWY LIVMA   
               
               
                   
               
               
                 B62 
                   
                   
                 
                   1° Anchor 
                 
                   
                   
                   
                   
                   
                   
                 
                   1° Anchor 
                 
               
               
                   
                   
                   
                 QL IVMP   
                   
                   
                   
                   
                   
                   
                 FWY MIVLA   
               
               
                   
               
               
                 Italicized residues indicate less preferred or “tolerated” residues 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IV (E) 
               
             
            
               
                   
               
               
                 HLA Class I Motifs 
               
            
           
           
               
               
            
               
                   
                 POSITION 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 9 or C- 
                 C- 
               
               
                   
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
                 7 
                 8 
                 terminus 
                 terminus 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 A1 
                 preferred 
                 GFYW 
                 
                   1° Anchor 
                 
                 DEA 
                 YFW 
                   
                 P 
                 DEQN 
                 YFW 
                 
                   1° Anchor 
                 
                   
               
               
                 9-mer 
                   
                   
                 STM 
                   
                   
                   
                   
                   
                   
                 Y 
                   
               
               
                   
                 deleterious 
                 DE 
                   
                 RHKLIV 
                 A 
                 G 
                 A 
                   
                   
                   
                   
               
               
                   
                   
                   
                   
                 MP 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A1 
                 preferred 
                 GRHK 
                 ASTCLIVM 
                 
                   1° Anchor 
                 
                 GSTC 
                   
                 ASTC 
                 LIVM 
                 DE 
                 
                   1° Anchor 
                 
                   
               
               
                 9-mer 
                   
                   
                   
                 DE AS   
                   
                   
                   
                   
                   
                 Y 
                   
               
               
                   
                 deleterious 
                 A 
                 RHKDEP 
                   
                 DE 
                 PQN 
                 RHK 
                 PG 
                 GP 
                   
                   
               
               
                   
                   
                   
                 YFW 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A1 
                 preferred 
                 YFW 
                 
                   1° Anchor 
                 
                 DEAQN 
                 A 
                 YFWQN 
                   
                 PASTC 
                 GDE 
                 P 
                 
                   1° Anchor 
                 
               
               
                 10-mer 
                   
                   
                 STM 
                   
                   
                   
                   
                   
                   
                   
                 Y 
               
               
                   
                 deleterious 
                 GP 
                   
                 RHKGLI 
                 DE 
                 RHK 
                 QNA 
                 RHKYFW 
                 RHK 
                 A 
                   
               
               
                   
                   
                   
                   
                 VM 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A1 
                 preferred 
                 YFW 
                 STCLIVM 
                 
                   1° Anchor 
                 
                 A 
                 YFW 
                   
                 PG 
                 G 
                 YFW 
                 
                   1° Anchor 
                 
               
               
                 10-mer 
                   
                   
                   
                 DE AS   
                   
                   
                   
                   
                   
                   
                 Y 
               
               
                   
                 deleterious 
                 RHK 
                 RHKDEP 
                   
                   
                 P 
                 G 
                   
                 PRHK 
                 QN 
                   
               
               
                   
                   
                   
                 YFW 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A2.1 
                 preferred 
                 YFW 
                 
                   1° Anchor 
                 
                 YFW 
                 STC 
                 YFW 
                   
                 A 
                 P 
                 
                   1° Anchor 
                 
                   
               
               
                 9-mer 
                   
                   
                 LM IVQAT   
                   
                   
                   
                   
                   
                   
                 V LIMAT   
                   
               
               
                   
                 deleterious 
                 DEP 
                   
                 DERKH 
                   
                   
                 RKH 
                 DERKH 
                   
                   
                   
               
               
                   
               
               
                 A2.1 
                 preferred 
                 AYFW 
                 
                   1° Anchor 
                 
                 LVIM 
                 G 
                   
                 G 
                   
                 FYWL 
                   
                 
                   1° Anchor 
                 
               
               
                 10-mer 
                   
                   
                 LM IVQAT   
                   
                   
                   
                   
                   
                 VIM 
                   
                 V LIMAT   
               
               
                   
                 deleterious 
                 DEP 
                   
                 DE 
                 RKHA 
                 P 
                   
                 RKH 
                 DER 
                 RKH 
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 KH 
                   
                   
               
               
                   
               
               
                 A3 
                 preferred 
                 RHK 
                 
                   1° Anchor 
                 
                 YFW 
                 PRHKY 
                 A 
                 YFW 
                   
                 P 
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                   
                 LMVISA 
                   
                 FW 
                   
                   
                   
                   
                 KYR HFA   
                   
               
               
                   
                   
                   
                 TFCGD 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 deleterious 
                 DEP 
                   
                 DE 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A11 
                 preferred 
                 A 
                 
                   1° Anchor 
                 
                 YFW 
                 YFW 
                 A 
                 YFW 
                 YFW 
                 P 
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                   
                 VTLMIS 
                   
                   
                   
                   
                   
                   
                 K RYH   
                   
               
               
                   
                   
                   
                 AGN CDF   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 deleterious 
                 DEP 
                   
                   
                   
                   
                   
                 A 
                 G 
                   
                   
               
               
                   
               
               
                 A24 
                 preferred 
                 YFWRHK 
                 
                   1° Anchor 
                 
                   
                 STC 
                   
                   
                 YFW 
                 YFW 
                 
                   1° Anchor 
                 
                   
               
               
                 9-mer 
                   
                   
                 YFW M   
                   
                   
                   
                   
                   
                   
                 FLIW 
                   
               
               
                   
                 deleterious 
                 DEG 
                   
                 DE 
                 G 
                 QNP 
                 DER 
                 G 
                 AQN 
                   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 HK 
                   
                   
                   
                   
               
               
                   
               
               
                 A24 
                 preferred 
                   
                 
                   1° Anchor 
                 
                   
                 P 
                 YFWP 
                   
                 P 
                   
                   
                 
                   1° Anchor 
                 
               
               
                 10-mer 
                   
                   
                 YFW M   
                   
                   
                   
                   
                   
                   
                   
                 FLIW 
               
               
                   
                 deleterious 
                   
                   
                 GDE 
                 QN 
                 RHK 
                 DE 
                 A 
                 QN 
                 DEA 
                   
               
               
                   
               
               
                 A3101 
                 preferred 
                 RHK 
                 
                   1° Anchor 
                 
                 YFW 
                 P 
                   
                 YFW 
                 YFW 
                 AP 
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                   
                 MVT ALIS   
                   
                   
                   
                   
                   
                   
                 R K   
                   
               
               
                   
                 deleterious 
                 DEP 
                   
                 DE 
                   
                 ADE 
                 DE 
                 DE 
                 DE 
                   
                   
               
               
                   
               
               
                 A3301 
                 preferred 
                   
                 
                   1° Anchor 
                 
                 YFW 
                   
                   
                   
                 AYFW 
                   
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                   
                 MVALF I   
                   
                   
                   
                   
                   
                   
                 RK 
                   
               
               
                   
                   
                   
                 
                   ST 
                 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
                 deleterious 
                 GP 
                   
                 DE 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A1 
                 preferred 
                 GFYW 
                 
                   1° Anchor 
                 
                 DEA 
                 YFW 
                   
                 P 
                 DEQN 
                 YFW 
                 
                   1° Anchor 
                 
                   
               
               
                 9-mer 
                   
                   
                 STM 
                   
                   
                   
                   
                   
                   
                 Y 
                   
               
               
                   
                 deleterious 
                 DE 
                   
                 RHKLIV 
                 A 
                 G 
                 A 
                   
                   
                   
                   
               
               
                   
                   
                   
                   
                 MP 
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A1 
                 preferred 
                 GRHK 
                 ASTCLIVM 
                 
                   1° Anchor 
                 
                 GSTC 
                   
                 ASTC 
                 LIVM 
                 DE 
                 
                   1° Anchor 
                 
                   
               
               
                 9-mer 
                   
                   
                   
                 DE AS   
                   
                   
                   
                   
                   
                 Y 
                   
               
               
                   
                 deleterious 
                 A 
                 RHKDEP 
                   
                 DE 
                 PQN 
                 RHK 
                 PG 
                 GP 
                   
                   
               
               
                   
                   
                   
                 YFW 
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 A6801 
                 preferred 
                 YFWSTC 
                 
                   1° Anchor 
                 
                   
                   
                 YFWLI 
                   
                 YFW 
                 P 
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                   
                 AVT MSLI   
                   
                   
                 VM 
                   
                   
                   
                 RK 
                   
               
               
                   
                 deleterious 
                 GP 
                   
                 DEG 
                   
                 RHK 
                   
                   
                 A 
                   
                   
               
               
                   
               
               
                 B0702 
                 preferred 
                 RHKFWY 
                 
                   1° Anchor 
                 
                 RHK 
                   
                 RHK 
                 RHK 
                 RHK 
                 PA 
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                   
                 P 
                   
                   
                   
                   
                   
                   
                 LMF WY   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 
                   AIV 
                 
                   
               
               
                   
                 deleterious 
                 DEQNP 
                   
                 DEP 
                 DE 
                 DE 
                 GDE 
                 QN 
                 DE 
                   
                   
               
               
                   
               
               
                 B3501 
                 preferred 
                 FWYLI 
                 
                   1° Anchor 
                 
                 FWY 
                   
                   
                   
                 FWY 
                   
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                 VM 
                 P 
                   
                   
                   
                   
                   
                   
                 LMFWY 
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 
                   IVA 
                 
                   
               
               
                   
                 deleterious 
                 AGP 
                   
                   
                   
                 G 
                 G 
                   
                   
                   
                   
               
               
                   
               
               
                 B51 
                 preferred 
                 LIVMF 
                 
                   1° Anchor 
                 
                 FWY 
                 STC 
                 FWY 
                   
                 G 
                 FWY 
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                 WY 
                 P 
                   
                   
                   
                   
                   
                   
                 LIVFWY 
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 
                   AM 
                 
                   
               
               
                   
                 deleterious 
                 AGPDE 
                   
                   
                   
                 DE 
                 G 
                 DEQN 
                 GDE 
                   
                   
               
               
                   
                   
                 RHKSTC 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                   
               
               
                 B5301 
                 preferred 
                 LIVMF 
                 
                   1° Anchor 
                 
                 FWY 
                 STC 
                 FWY 
                   
                 LIVMF 
                 FWY 
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                 WY 
                 P 
                   
                   
                   
                   
                 WY 
                   
                 IMFWY 
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 
                   ALV 
                 
                   
               
               
                   
                 deleterious 
                 AGPQN 
                   
                   
                   
                   
                 G 
                 RHKQN 
                 DE 
                   
                   
               
               
                   
               
               
                 B5401 
                 preferred 
                 FWY 
                 
                   1° Anchor 
                 
                 FWYLIVM 
                   
                 LIVM 
                   
                 ALIVM 
                 FWY 
                 
                   1° Anchor 
                 
                   
               
               
                   
                   
                   
                 P 
                   
                   
                   
                   
                   
                 AP 
                 ATIV LM   
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 
                   FWY 
                 
                   
               
               
                   
                 deleterious 
                 GPQNDE 
                   
                 GDESTC 
                   
                 RHKDE 
                 DE 
                 QNDGE 
                 DE 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IV (F) 
               
             
            
               
                   
               
               
                 Summary of HLA-supertypes 
               
               
                 Overall phenotypic frequencies of HLA-supertypes in different ethnic populations 
               
            
           
           
               
               
            
               
                 Specificity 
                 Phenotypic frequency 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Supertype 
                 Position 2 
                 C-Terminus 
                 Caucasian 
                 N.A. Black 
                 Japanese 
                 Chinese 
                 Hispanic 
                 Average 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 B7 
                 P 
                 AILMVFWY 
                 43.2 
                 55.1 
                 57.1 
                 43.0 
                 49.3 
                 49.5 
               
               
                 A3 
                 AILMVST 
                 RK 
                 37.5 
                 42.1 
                 45.8 
                 52.7 
                 43.1 
                 44.2 
               
               
                 A2 
                 AILMVT 
                 AILMVT 
                 45.8 
                 39.0 
                 42.4 
                 45.9 
                 43.0 
                 42.2 
               
               
                 A24 
                 YF (WIVLMT) 
                 FI (YWLM) 
                 23.9 
                 38.9 
                 58.6 
                 40.1 
                 38.3 
                 40.0 
               
               
                 B44 
                 E (D) 
                 FWYLIMVA 
                 43.0 
                 21.2 
                 42.9 
                 39.1 
                 39.0 
                 37.0 
               
               
                 A1 
                 TI (LVMS) 
                 FWY 
                 47.1 
                 16.1 
                 21.8 
                 14.7 
                 26.3 
                 25.2 
               
               
                 B27 
                 RHK 
                 FYL (WMI) 
                 28.4 
                 26.1 
                 13.3 
                 13.9 
                 35.3 
                 23.4 
               
               
                 B62 
                 QL (IVMP) 
                 FWY (MIV) 
                 12.6 
                 4.8 
                 36.5 
                 25.4 
                 11.1 
                 18.1 
               
               
                 B58 
                 ATS 
                 FWY (LIV) 
                 10.0 
                 25.1 
                 1.6 
                 9.0 
                 5.9 
                 10.3 
               
               
                   
               
            
           
         
       
     
                     TABLE IV (G)                  Calculated population coverage afforded by different       HLA-supertype combinations                         Phenotypic frequency                                         HLA-   Cau-   N.A           His-           supertypes   casian   Blacks   Japanese   Chinese   panic   Average                                                 A2, A3 and B7   83.0   86.1   87.5   88.4   86.3   86.2       A2, A3, B7,   99.5   98.1   100.0   99.5   99.4   99.3       A24, B44 and       A1       A2, A3, B7,   99.9   99.6   100.0   99.8   99.9   99.8       A24, B44, A1,       B27, B62, and       B 58                    
Motifs indicate the residues defining supertype specificities. The motifs incorporate residues determined on the basis of published data to be recognized by multiple alleles within the supertype. Residues within brackets are additional residues also predicted to be tolerated by multiple alleles within the supertype.
 
     
       
         
           
               
             
               
                 TABLE V 
               
             
            
               
                   
               
               
                 Frequently Occurring Motifs 
               
            
           
           
               
               
               
               
            
               
                   
                 avrg. % 
                   
                   
               
               
                 Name 
                 identity 
                 Description 
                 Potential Function 
               
               
                   
               
               
                 zf-C2H2 
                 34% 
                 Zinc finger, C2H2 type 
                 Nucleic acid-binding protein functions as 
               
               
                   
                   
                   
                 transcription factor, nuclear location probable 
               
               
                 cytochrome_b_N 
                 68% 
                 Cytochrome b(N- 
                 membrane bound oxidase, generate superoxide 
               
               
                   
                   
                 terminal)/b6/petB 
                   
               
               
                 Ig 
                 19% 
                 Immunoglobulin domain 
                 domains are one hundred amino acids long and 
               
               
                   
                   
                   
                 include a conserved intradomain disulfide bond. 
               
               
                 WD40 
                 18% 
                 WD domain, G-beta repeat 
                 tandem repeats of about 40 residues, each 
               
               
                   
                   
                   
                 containing a Trp-Asp motif. Function in signal 
               
               
                   
                   
                   
                 transduction and protein interaction 
               
               
                 PDZ 
                 23% 
                 PDZ domain 
                 may function in targeting signaling molecules to 
               
               
                   
                   
                   
                 sub-membranous sites 
               
               
                 LRR 
                 28% 
                 Leucine Rich Repeat 
                 short sequence motifs involved in protein-protein 
               
               
                   
                   
                   
                 interactions 
               
               
                 Pkinase 
                 23% 
                 Protein kinase domain 
                 conserved catalytic core common to both 
               
               
                   
                   
                   
                 serine/threonine and tyrosine protein kinases 
               
               
                   
                   
                   
                 containing an ATP binding site and a catalytic 
               
               
                   
                   
                   
                 site 
               
               
                 PH 
                 16% 
                 PH domain 
                 pleckstrin homology involved in intracellular 
               
               
                   
                   
                   
                 signaling or as constituents of the cytoskeleton 
               
               
                 EGF 
                 34% 
                 EGF-like domain 
                 30-40 amino-acid long found in the extracellular 
               
               
                   
                   
                   
                 domain of membrane-bound proteins or in 
               
               
                   
                   
                   
                 secreted proteins 
               
               
                 Rvt 
                 49% 
                 Reverse transcriptase 
                   
               
               
                   
                   
                 (RNA-dependent DNA 
                   
               
               
                   
                   
                 polymerase) 
                   
               
               
                 Ank 
                 25% 
                 Ank repeat 
                 Cytoplasmic protein, associates integral 
               
               
                   
                   
                   
                 membrane proteins to the cytoskeleton 
               
               
                 Oxidored_q1 
                 32% 
                 NADH- 
                 membrane associated. Involved in proton 
               
               
                   
                   
                 Ubiquinone/plastoquinone 
                 translocation across the membrane 
               
               
                   
                   
                 (complex I), various chains 
                   
               
               
                 Efhand 
                 24% 
                 EF hand 
                 calcium-binding domain, consists of a12 residue 
               
               
                   
                   
                   
                 loop flanked on both sides by a 12 residue alpha- 
               
               
                   
                   
                   
                 helical domain 
               
               
                 Rvp 
                 79% 
                 Retroviral aspartyl 
                 Aspartyl or acid proteases, centered on a 
               
               
                   
                   
                 protease 
                 catalytic aspartyl residue 
               
               
                 Collagen 
                 42% 
                 Collagen triple helix repeat 
                 extracellular structural proteins involved in 
               
               
                   
                   
                 (20 copies) 
                 formation of connective tissue. The sequence 
               
               
                   
                   
                   
                 consists of the G-X-Y and the polypeptide chains 
               
               
                   
                   
                   
                 forms a triple helix. 
               
               
                 Fn3 
                 20% 
                 Fibronectin type III domain 
                 Located in the extracellular ligand-binding 
               
               
                   
                   
                   
                 region of receptors and is about 200 amino acid 
               
               
                   
                   
                   
                 residues long with two pairs of cysteines 
               
               
                   
                   
                   
                 involved in disulfide bonds 
               
               
                 7tm_1 
                 19% 
                 7 transmembrane receptor 
                 seven hydrophobic transmembrane regions, with 
               
               
                   
                   
                 (rhodopsin family) 
                 the N-terminus located extracellularly while the 
               
               
                   
                   
                   
                 C-terminus is cytoplasmic. Signal through G 
               
               
                   
                   
                   
                 proteins 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE VI 
               
               
                   
               
               
                 Motif-bearing subsequences and Post-Translational 
               
               
                 Modifications of 158P3D2 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Phosphorylation sites predicted: 
               
               
                 Phosphoserine predictions 
               
            
           
           
               
               
            
               
                 Pos. 
                 Context 
               
               
                   
               
               
                 73 
                 SRVFSLRPL (SEQ ID NO: 107) 
               
               
                 107 
                 NLQVSPIQV (SEQ ID NO: 108) 
               
               
                 128 
                 TGAWSEEDF (SEQ ID NO: 109) 
               
               
                 173 
                 RLARSLGQQ (SEQ ID NO: 110) 
               
               
                 206 
                 GVMFSPLKS (SEQ ID NO: 111) 
               
               
                 210 
                 SPLKSRARA (SEQ ID NO: 112) 
               
               
                 268 
                 QRGTSCPFY (SEQ ID NO: 113) 
               
               
                 320 
                 LTPPSPKAF (SEQ ID NO: 114) 
               
               
                 380 
                 KVTLSVRAR (SEQ ID NO: 115) 
               
               
                 460 
                 YVRVSFLGQ (SEQ ID NO: 116) 
               
               
                 469 
                 EGETSVSAE (SEQ ID NO: 117) 
               
               
                 471 
                 ETSVSAEAA (SEQ ID NO: 118) 
               
               
                 484 
                 NEQLSFVEL (SEQ ID NO: 119) 
               
               
                 522 
                 LRRISHPGR (SEQ ID NO: 120) 
               
               
                 624 
                 HLDASPGAE (SEQ ID NO: 121) 
               
               
                 679 
                 SQPISFEIS (SEQ ID NO: 122) 
               
               
                 683 
                 SFEISIGRA (SEQ ID NO: 123) 
               
               
                 916 
                 DVLFSVVEE (SEQ ID NO: 124) 
               
               
                 982 
                 GLPSSLHRD (SEQ ID NO: 125) 
               
               
                 1012 
                 DSGLSDPFA (SEQ ID NO: 126) 
               
               
                 1127 
                 ELDYSGRLE (SEQ ID NO: 127) 
               
               
                 1136 
                 PSVPSEVEP (SEQ ID NO: 128) 
               
               
                 1151 
                 VEPHSGRLS (SEQ ID NO: 129) 
               
               
                 1155 
                 SGRLSLPPN (SEQ ID NO: 130) 
               
               
                 1208 
                 EVLASYRES (SEQ ID NO: 131) 
               
               
                 1212 
                 SYRESPNFT (SEQ ID NO: 132) 
               
               
                 1380 
                 DPGDSDGVN (SEQ ID NO: 133) 
               
               
                 1405 
                 KGTVSPKKA (SEQ ID NO: 134) 
               
               
                 1419 
                 IYNRSLKEE (SEQ ID NO: 135) 
               
               
                 1519 
                 YVVVSAGRE (SEQ ID NO: 136) 
               
               
                 1585 
                 NRFYSHHRA (SEQ ID NO: 137) 
               
               
                 1595 
                 CGLASQYEV (SEQ ID NO: 138) 
               
               
                 1652 
                 LPPGSSSPT (SEQ ID NO: 139) 
               
               
                 1654 
                 PGSSSPTVA (SEQ ID NO: 140) 
               
               
                 1699 
                 YHPHSPGLL (SEQ ID NO: 141) 
               
               
                 1733 
                 RQPISYELR (SEQ ID NO: 142) 
               
               
                 1809 
                 EREVSVWRR (SEQ ID NO: 143) 
               
               
                 1814 
                 VWRRSGPFA (SEQ ID NO: 144) 
               
               
                 1841 
                 YDRISANDF (SEQ ID NO: 145) 
               
               
                 1975 
                 LEKPSRPKT (SEQ ID NO: 146) 
               
               
                   
               
            
           
           
               
            
               
                 Phosphothreonine predictions 
               
            
           
           
               
               
            
               
                 29 
                 FRGFTQKTR (SEQ ID NO: 147) 
               
               
                 261 
                 QRRVTATQR (SEQ ID NO: 148) 
               
               
                 263 
                 RVTATQRGT (SEQ ID NO: 149) 
               
               
                 267 
                 TQRGTSCPF (SEQ ID NO: 150) 
               
               
                 338 
                 TRIGTFRMD (SEQ ID NO: 151) 
               
               
                 367 
                 DPRDTRAGT (SEQ ID NO: 152) 
               
               
                 601 
                 LSRLTRKKK (SEQ ID NO: 153) 
               
               
                 613 
                 RRDQTPKAV (SEQ ID NO: 154) 
               
               
                 704 
                 AGEGTEGAA (SEQ ID NO: 155) 
               
               
                 828 
                 RRTMTRPNA (SEQ ID NO: 156) 
               
               
                 1022 
                 VLISTQCQT (SEQ ID NO: 157) 
               
               
                 1026 
                 TQCQTTRVL (SEQ ID NO: 158) 
               
               
                 1033 
                 VLEQTLSPL (SEQ ID NO: 159) 
               
               
                 1223 
                 VRHLTVVFK (SEQ ID NO: 160) 
               
               
                 1412 
                 KAVATLKIY (SEQ ID NO: 161) 
               
               
                 1527 
                 ERQDTKERY (SEQ ID NO: 162) 
               
               
                 1557 
                 ETELTVAVF (SEQ ID NO: 163) 
               
               
                 1643 
                 KVFLTPPET (SEQ ID NO: 164) 
               
               
                 1743 
                 VIWNTEDVV (SEQ ID NO: 165) 
               
               
                 1804 
                 DYLPTEREV (SEQ ID NO: 166) 
               
               
                 1941 
                 VYILTGKVE (SEQ ID NO: 167) 
               
               
                 1952 
                 FELLTVEEA (SEQ ID NO: 168) 
               
               
                 1979 
                 SRPKTSFNW (SEQ ID NO: 169) 
               
               
                   
               
            
           
           
               
            
               
                 Phosphotyrosine predictions 
               
            
           
           
               
               
            
               
                 52 
                 RWPHYGAPL (SEQ ID NO: 170) 
               
               
                 456 
                 LVEPYVRVS (SEQ ID NO: 171) 
               
               
                 542 
                 WVPLYGSPP (SEQ ID NO: 172) 
               
               
                 990 
                 DDFSYFQLR (SEQ ID NO: 173) 
               
               
                 1095 
                 MEDPYQRPE (SEQ ID NO: 174) 
               
               
                 1243 
                 PEQPYLQPP (SEQ ID NO: 175) 
               
               
                 1515 
                 KADPYVVVS (SEQ ID NO: 176) 
               
               
                 1531 
                 TKERYIPKQ (SEQ ID NO: 177) 
               
               
                 1602 
                 EVDGYNAWR (SEQ ID NO: 178) 
               
               
                 1629 
                 PAPEYRAGA (SEQ ID NO: 179) 
               
               
                 1763 
                 SSDIYVKSW (SEQ ID NO: 180) 
               
               
                   
               
            
           
           
               
            
               
                 N-glycosylation sites 
               
            
           
           
               
               
            
               
                 67 
                 NCSR (SEQ ID NO: 181) 
               
               
                 1214 
                 NFTE (SEQ ID NO: 182) 
               
               
                 1417 
                 NRSL (SEQ ID NO: 183) 
               
               
                   
               
            
           
           
               
            
               
                 O-glycosylation sites 
               
            
           
           
               
               
            
               
                 1403 
                 T 
               
               
                 1647 
                 T 
               
               
                 1656 
                 T 
               
               
                   
               
            
           
           
               
            
               
                 Dileucine motifs 
               
               
                 LL at 290 
               
               
                 LL at 291 
               
               
                 LL at 406 
               
               
                 LL at 407 
               
               
                 LL at 438 
               
               
                 LL at 571 
               
               
                 LL at 572 
               
               
                 LL at 643 
               
               
                 LL at 658 
               
               
                 LL at 714 
               
               
                 LL at 840 
               
               
                 LL at 847 
               
               
                 LL at 855 
               
               
                 LL at 874 
               
               
                 LL at 971 
               
               
                 LL at 1041 
               
               
                 LL at 1184 
               
               
                 LL at 1320 
               
               
                 LL at 1333 
               
               
                 LL at 1491 
               
               
                   
               
               
                 C2 domains 
               
               
                 aa2-96 
               
               
                 aa230-355 
               
               
                 aa420-530 
               
               
                 aa992-1100 
               
               
                 aa1492-1591 
               
               
                 aa1735-1865 
               
               
                   
               
               
                 Coiled-coil regions 
               
               
                 aa168-186 
               
               
                 aa1895-1922 
               
               
                   
               
               
                 Interaction motifs and specific kinase 
               
               
                 phosphorylation sites 
               
               
                 proteins interaction motif 2 
               
               
                 RRTMTRP (SEQ ID NO: 184) 
               
               
                   
               
               
                 FHA domain interaction motif 1, threonine 
               
               
                 phosphorylation is required 
               
               
                 TPKA (SEQ ID NO: 185) 
               
               
                 TEGA (SEQ ID NO: 186) 
               
               
                 TWRL (SEQ ID NO: 187) 
               
               
                 TSEL (SEQ ID NO: 188) 
               
               
                 TAPL (SEQ ID NO: 189) 
               
               
                 TLKI (SEQ ID NO: 190) 
               
               
                 TRPL (SEQ ID NO: 191) 
               
               
                   
               
               
                 Motif found in p53 family members which confers 
               
               
                 binding to the N-terminal domain of MDM2 
               
               
                 FGPAWVPL (SEQ ID NO:192) 
               
               
                   
               
               
                 Class III PDZ domains binding motif 
               
               
                 DENL (SEQ ID NO: 193) 
               
               
                 FELI (SEQ ID NO: 194) 
               
               
                 RDSL (SEQ ID NO: 195) 
               
               
                 NEGV (SEQ ID NO: 196) 
               
               
                 VEEL (SEQ ID NO: 197) 
               
               
                 EELL (SEQ ID NO: 198) 
               
               
                 PENV (SEQ ID NO: 199) 
               
               
                 EDFL (SEQ ID NO: 200) 
               
               
                 EEQL (SEQ ID NO: 201) 
               
               
                 EEEL (SEQ ID NO: 202) 
               
               
                 AERL (SEQ ID NO: 203) 
               
               
                 VERL (SEQ ID NO: 204) 
               
               
                 LEVL (SEQ ID NO: 205) 
               
               
                 PDVL (SEQ ID NO: 206) 
               
               
                 QDVL (SEQ ID NO: 207) 
               
               
                 PDLL (SEQ ID NO: 208) 
               
               
                 GELI (SEQ ID NO: 209) 
               
               
                 VEVL (SEQ ID NO: 210) 
               
               
                 SEVL (SEQ ID NO: 211) 
               
               
                 TELV (SEQ ID NO: 212) 
               
               
                 EEDI (SEQ ID NO: 213) 
               
               
                 PEEL (SEQ ID NO: 214) 
               
               
                 SDGV (SEQ ID NO: 215) 
               
               
                 EDWL (SEQ ID NO: 216) 
               
               
                 SEAV (SEQ ID NO: 217) 
               
               
                 PETL (SEQ ID NO: 218) 
               
               
                 PEHV (SEQ ID NO: 219) 
               
               
                 PEDL (SEQ ID NO: 220) 
               
               
                 FELL (SEQ ID NO: 221) 
               
               
                 PEPL (SEQ ID NO: 222) 
               
               
                   
               
               
                 Src-family Src Homology 2 (SH2) domains binding 
               
               
                 motif. 
               
               
                 YQPP (SEQ ID NO: 223) 
               
               
                   
               
               
                 This is the motif recognized by class I SH3 
               
               
                 domains 
               
               
                 RAEPEPP (SEQ ID NO: 224) 
               
               
                 RLEPSVP (SEQ ID NO: 225) 
               
               
                   
               
               
                 This is the motif recognized by those SH3 domains 
               
               
                 with a non-canonical class I recognition 
               
               
                 specificity 
               
               
                 LPPPMLP (SEQ ID NO: 226) 
               
               
                 PMLPPAP (SEQ ID NO: 227) 
               
               
                 RAEPEPP (SEQ ID NO: 228) 
               
               
                 AEGPEIP (SEQ ID NO: 229) 
               
               
                 PENVLAP (SEQ ID NO: 230) 
               
               
                 AEEPQPP (SEQ ID NO: 231) 
               
               
                 EPQPPLP (SEQ ID NO: 232) 
               
               
                 RLEPSVP (SEQ ID NO: 233) 
               
               
                 LLEVEQP (SEQ ID NO: 234) 
               
               
                 QDLPEQP (SEQ ID NO: 235) 
               
               
                 DMMPKGP (SEQ ID NO: 236) 
               
               
                 LYHPHSP (SEQ ID NO: 237) 
               
               
                 PQDVPAP (SEQ ID NO: 238) 
               
               
                 QDVPAPP (SEQ ID NO: 239) 
               
               
                 DIKPRQP (SEQ ID NO: 240) 
               
               
                 LEKPSRP (SEQ ID NO: 241) 
               
               
                   
               
               
                 This is the motif recognized by those SH3 domains 
               
               
                 with a non-canonical class II recognition 
               
               
                 specificity 
               
               
                 KPPLKKLP (SEQ ID NO: 242) 
               
               
                   
               
               
                 Major TRAF2-binding consensus motif. Members of 
               
               
                 the tumor necrosis factor receptor (TNFR) 
               
               
                 superfamily initiate intracellular signaling by 
               
               
                 recruiting the C-domain of the TNFR-associated 
               
               
                 factors (TRAFs) through their cytoplasmic tails. 
               
               
                 PEEE (SEQ ID NO: 243) 
               
               
                 PPEE (SEQ ID NO: 244) 
               
               
                 PEEE (SEQ ID NO: 245) 
               
               
                 SLQE (SEQ ID NO: 246) 
               
               
                 TVEE (SEQ ID NO: 247) 
               
               
                   
               
               
                 TRAF6 binding site. Members of the tumor necrosis 
               
               
                 factor receptor (TNFR) superfamily initiate intra- 
               
               
                 cellular signaling by recruiting the C-domain of 
               
               
                 the TNFR-associated factors (TRAFs) through their 
               
               
                 cytoplasmatic tails. 
               
               
                 GVPAERPWA (SEQ ID NO: 248) 
               
               
                 ARPEEEKEE (SEQ ID NO: 249) 
               
               
                 DPPEEEGEM (SEQ ID NO: 250) 
               
               
                 PDPEELDWG (SEQ ID NO: 251) 
               
               
                   
               
               
                 PPLP is the motif recognized by WW domains of 
               
               
                 Group II 
               
               
                 PPLP (SEQ ID NO: 252) 
               
               
                   
               
               
                 GSK3 phosphorylation recognition site 
               
               
                 GATGAWS (SEQ ID NO: 253) 
               
               
                 MFSPLKS (SEQ ID NO: 254) 
               
               
                 RDTRAGT (SEQ ID NO: 255) 
               
               
                 VASQPIS (SEQ ID NO: 256) 
               
               
                 PISFEIS (SEQ ID NO: 257) 
               
               
                 IQSLMLT (SEQ ID NO: 258) 
               
               
                 PHSGRLS (SEQ ID NO: 259) 
               
               
                 LASYRES (SEQ ID NO: 260) 
               
               
                 RESPNFT (SEQ ID NO: 261)  
               
               
                 FLTPPET (SEQ ID NO: 262) 
               
               
                 PGSSSPT (SEQ ID NO: 263) 
               
               
                 KPSRPKT (SEQ ID NO: 264) 
               
               
                   
               
               
                 PKA phosphorylation site 
               
               
                 RDT 
               
               
                 RDS 
               
               
                 RLT 
               
               
                 RGS 
               
               
                 RRT 
               
               
                 RLS 
               
               
                 RES 
               
               
                 RFT 
               
               
                   
               
               
                 Site phosphorylated by the Polo-like-kinase 
               
               
                 EATM (SEQ ID NO: 265) 
               
               
                 DPTV (SEQ ID NO: 266) 
               
               
                 EISI (SEQ ID NO: 267) 
               
               
                 DHTW (SEQ ID NO: 268) 
               
               
                 EPSA (SEQ ID NO: 269) 
               
               
                 EPSV (SEQ ID NO: 270) 
               
               
                   
               
               
                 Proline-Directed Kinase (e.g. MAPK) phosphoryla-  
               
               
                 tion site in higher eukaryotes. 
               
               
                 LQVSPIQ (SEQ ID NO: 271) 
               
               
                 VMFSPLK (SEQ ID NO: 272) 
               
               
                 LYGSPPG (SEQ ID NO: 273) 
               
               
                 RDQTPKA (SEQ ID NO: 274) 
               
               
                 LDASPGA (SEQ ID NO: 275) 
               
               
                 YRESPNF (SEQ ID NO: 276) 
               
               
                 GTVSPKK (SEQ ID NO: 277) 
               
               
                 VFLTPPE (SEQ ID NO: 278) 
               
               
                 GSSSPTV (SEQ ID NO: 279) 
               
               
                 HPHSPGL (SEQ ID NO: 280) 
               
               
                   
               
               
                 SH-PTP2 and phospholipase C-gamma Src Homology 2  
               
               
                 (SH2) domains binding motif. 
               
               
                 aa249-252 
               
               
                 aa456-459 
               
               
                 aa1515-1518 
               
               
                   
               
               
                 CK1 phosphorylation site 
               
               
                 aa1009-1012 
               
               
                 aa234-237 
               
               
                 aa484-487 
               
               
                 aa760-763 
               
               
                   
               
               
                 CK2 phosphorylation site 
               
               
                 aa1085-1807 
               
               
                   
               
               
                 PKB Phosphorylation site 
               
               
                 aa1522-1528 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE VII 
               
               
                   
               
               
                 Search Peptides 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 158P3D2 v.1, 9-mers, 10-mers and 15-mers (SEQ ID NO: 281) 
               
            
           
           
               
               
            
               
                 MWIDIFPQDV PAPPPVDIKP RQPISYELRV VIWNTEDVVL DDENPLTGEM SSDIYVKSWV 
                 60 
               
               
                 KGLEHDKQET DVHFNSLTGE GNFNWRFVFR FDYLPTEREV SVWRRSGPFA LEEAEFRQPA 
                 120 
               
               
                 VLVLQVWDYD RISANDFLGS LELQLPDMVR GARGPELCSV QLARNGAGPR CNLFRCRRLR 
                 180 
               
               
                 GWWPVVKLKE AEDVEREAQE AQAGKKKRKQ RRRKGRPEDL EFTDMGGNVY ILTGKVEAEF 
                 240 
               
               
                 ELLTVEEAEK RPVGKGRKQP EPLEKPSRPK TSFNWFVNPL KTFVFFIWRR YWRTLVLLLL 
                 300 
               
               
                 VLLTVFLLLV FYTIPGQISQ VIFRPLHK 
                 328 
               
               
                   
               
               
                 158P3D2 v.2A, 9-mers, 10-mers and 15-mers (SEQ ID NO: 282) 
                   
               
               
                 MDDPGDSDGV NLISMVGEIQ DQGEAEVKGT VSPKKAVATL KIYNRSLEEE FNHFEDWLNV 
                 60 
               
               
                 FPLYRGQGGQ DGGGEEEGSG HLVGKFKGSF LIYPESEAVL FSEPQISRGI PQNRPIKLLV 
                 120 
               
               
                 RVYVVKATNL APADPNGKAD PYVVVSAGRE RQDTKERYIP KQLNPIFGEI LELSISLPAE 
                 180 
               
               
                 TELTVAVFEH DLVGSDDLIG ETHIDLENRF YSHHRANCGL ASQYEVWVQQ GPQEPF 
                 236 
               
               
                   
               
               
                 158P3D2 v.3 
                   
               
               
                 9-mers aa 95-111 
                   
               
               
                 PTEREVSVRRRSGPFAL (SEQ ID NO: 283) 
                   
               
               
                 10-mers aa 94-112 
                   
               
               
                 LPTEREVSVRRRSGPFALE (SEQ ID NO: 284) 
                   
               
               
                 15-mers aa 89-117 
                   
               
               
                 FRFDYLPTEREVSVRRRSGPFALEEAEFR (SEQ ID NO: 285) 
                   
               
               
                   
               
               
                 158P3D2 v.4 
                   
               
               
                 9-mers aa 94-110 
                   
               
               
                 LPTEREVSIWRRSGPFA (SEQ ID NO: 286) 
                   
               
               
                 10-mers aa 93-111 
                   
               
               
                 YLPTEREVSIWRRSGPFAL(SEQ ID NO: 287) 
                   
               
               
                 15-mers aa 88-116 
                   
               
               
                 VFRFDYLPTEREVSIWRRSGPFALEEAEF (SEQ ID NO: 288) 
                   
               
               
                   
               
               
                 158P3D2 v.5A (BCP2A) ORF: 849-1385 
                   
               
               
                 9-mers 
                   
               
               
                 LVLQVWDYTASLPMTSLDPWSCSYQTWCVGPGAPSSALCSWPAMGPGRGAICFAAAA (SEQ ID NO: 289) 
                   
               
               
                 10-mers 
                   
               
               
                 VLVLQVWDYTASLPMTSLDPWSCSYQTWCVGPGAPSSALCSWPAMGPGRGAICFAAAA (SEQ ID NO: 290) 
                   
               
               
                 15-mers 
                   
               
               
                 FRQPAVLVLQVWDYTASLPMTSLDPWSCSYQTWCVGPGAPSSALCSWPAMGPGRGAICFAAAA 
                   
               
               
                 (SEQ ID NO: 291) 
                   
               
               
                   
               
               
                 158P3D2 v.10 
                   
               
               
                 9-mers aa 50-66 
                   
               
               
                 MSSDIYVKRWVKGLEHD (SEQ ID NO: 292) 
                   
               
               
                 10-mers aa 49-67 
                   
               
               
                 EMSSDIYVKRWVKGLEHDK (SEQ ID NO: 293) 
                   
               
               
                 15-mers aa 44-72 
                   
               
               
                 NPLTGEMSSDIYVKRWVKGLEHDKQETDV (SEQ ID NO: 294) 
                   
               
               
                   
               
               
                 158P3D2 v.12 
                   
               
               
                 9-mers aa 273-287 
                   
               
               
                 FNWFVNPLNTFVFFIWR (SEQ ID NO: 295) 
                   
               
               
                 10-mers aa 272-288 
                   
               
               
                 SFNWFVNPLNTFVFFIWRR (SEQ ID NO: 296) 
                   
               
               
                 15-mers aa 267-293 
                   
               
               
                 SRPKTSFNWFVNPLNTFVFFIWRRYWRTL (SEQ ID NO: 297) 
                   
               
               
                   
               
               
                 158P3D2 v.13 
                   
               
               
                 9-mers aa 274-288 
                   
               
               
                 NWFVNPLKAFVFFIWRR (SEQ ID NO: 298) 
                   
               
               
                 10-mers aa 273-287 
                   
               
               
                 FNWFVNPLKAFVFFIWRRY (SEQ ID NO: 299) 
                   
               
               
                 15-mers aa 268-294 
                   
               
               
                 RPKTSFNWFVNPLKAFVFFIWRRYWRTLV (SEQ ID NO: 300) 
                   
               
               
                   
               
               
                 Combination of v12 and v13 
                   
               
            
           
           
               
            
               
                 9-mers aa 273-287 
               
            
           
           
               
               
            
               
                 NWFVNPLNAFVFFIWR (SEQ ID NO: 301) 
                   
               
               
                 10-mers aa 272-288 
                   
               
               
                 FNWFVNPLNAFVFFIWRR (SEQ ID NO: 302) 
                   
               
               
                 15-mers aa 267-293 
                   
               
               
                 RPKTSFNWFVNPLNAFVFFIWRRYWRTL (SEQ ID NO: 303) 
                   
               
               
                   
               
               
                 v.14 ORF:65-4246 Frame +2 
                   
               
               
                 Part A 
                   
               
               
                 9-mers 
                   
               
               
                 ELVRHLTVDLPEQPYL (SEQ ID NO: 304) 
                   
               
               
                 10-mers 
                   
               
               
                 ELVRHLTVDLPEQPYLQ (SEQ ID NO: 305) 
                   
               
               
                 15-mers 
                   
               
               
                 ESPNFTELVRHLTVDLPEQPYLQPPLSI (SEQ ID NO: 306) 
                   
               
               
                   
               
               
                 Part B 
                   
               
               
                 9-mers 
                   
               
               
                 MVGEIQDQDLQQVPEGRI (SEQ ID NO: 307) 
                   
               
               
                 10-mers 
                   
               
               
                 SMVGEIQDQDLQQVPEGRI (SEQ ID NO: 308) 
                   
               
               
                 15-mers 
                   
               
               
                 GVNLISMVGEIQDQDLQQVPEGRI (SEQ ID NO: 309) 
                   
               
               
                   
               
               
                 v.15 ORF: 65-3502 Frame +2 
                   
               
               
                 Part A 
                   
               
               
                 9-mers 
                   
               
               
                 KLRFLAEEHNFDEDEM (SEQ ID NO: 310) 
                   
               
               
                 10-mers 
                   
               
               
                 AKLRFLAEEHNFDEDEM (SEQ ID NO: 311) 
                   
               
               
                 15-mers 
                   
               
               
                 AKKLLAKLRFLAEEHNFDEDEMDDPGDS (SEQ ID NO: 312) 
                   
               
               
                   
               
               
                 Part B 
                   
               
               
                 9-mers 
                   
               
               
                 LVRVYVVKLRNLCKIQGHEDFCLFSAATNLAPAD (SEQ ID NO: 313) 
                   
               
               
                 10-mers 
                   
               
               
                 LLVRVYVVKLRNLCKIQGHEDFCLFSAATNLAPADP (SEQ ID NO: 314) 
                   
               
               
                 15-mers 
                   
               
               
                 NRPIKLLVRVYVVKLRNLCKIQGHEDFCLFSAATNLAPADPNGKAD (SEQ ID NO: 315) 
                   
               
               
                   
               
               
                 Part C 
                   
               
               
                 9-mers 
                   
               
               
                 GLASQYEVWVQQGPQEPF (SEQ ID NO: 316) 
                   
               
               
                 10-mers 
                   
               
               
                 CGLASQYEVWVQQGPQEPF (SEQ ID NO: 317) 
                   
               
               
                 15-mers 
                   
               
               
                 HHRANCGLASQYEVWVQQGPQEPF (SEQ ID NO: 318) 
                   
               
               
                   
               
               
                 v.16 ORF:65-6037 Frame +2 1990 AA 
                   
               
               
                 Part A 
                   
               
               
                 9-mers 
                   
               
               
                 GSKVFLTPPETLPPVASGDPEEAQALLV (SEQ ID NO: 319) 
                   
               
               
                 10-mers 
                   
               
               
                 GSKVFLTPPETLPPVASGDPEEAQALLV (SEQ ID NO: 320) 
                   
               
               
                 15-mers 
                   
               
               
                 GSKVFLTPPETLPPVASGDPEEAQALLV (SEQ ID NO: 321) 
                   
               
               
                   
               
               
                 Part B 
                   
               
               
                 9-mers 
                   
               
            
           
           
               
            
               
                 VKLKEAEDGKVEAEFE (SEQ ID NO: 322) 
               
            
           
           
               
               
            
               
                 10mers 
                   
               
               
                 VVKLKEAEDGKVEAEFEL (SEQ ID NO: 323) 
                   
               
               
                 15-mers 
                   
               
               
                 RGWWPVVKLKEAEDGKVEAEFELLTVEE (SEQ ID NO: 324) 
                   
               
               
                   
               
               
                 v.17 ORF:65-6175 Frame +2 (SEQ ID NO: 325) 
                   
               
               
                 9-mers, 10-mers and 15-mers 
                   
               
               
                 MALTVSVQRL TGLTGTHDRQ VKLTFRGFTQ KTRKIHCGPE ADIGELFRWP HYGAPLAGEC 
                 60 
               
               
                 LSVQVVNCSR VFSLRPLGTL VISLQQLQNA GHLVLREALV DENLQVSPIQ VELDLKYQPP 
                 120 
               
               
                 EGATGAWSEE DFGAPIQDSF ELIIPNVGFQ ELEPGEAQLE RRAVALGRRL ARSLGQQDDE 
                 180 
               
               
                 ENELELELEQ DLDDEPDVEL SGVMFSPLKS RARALAHGDP FQVSRAQDFQ VGVTVLEAQK 
                 240 
               
               
                 LVGVNINPYV AVQVGGQRRV TATQRGTSCP FYNEYFLFEF HDTRLRLQDL LLEITVSGVG 
                 300 
               
               
                 VTSVLQRRGD EKAAGLTPPS PKAFHSQTLP FMATRIGTFR MDLGIILDQP DGQFYQRWVP 
                 360 
               
               
                 LHDPRDTRAG TKGFIKVTLS VRARGDLPPP MLPPAPGHCS DIEKNLLLPR GVPAERPWAR 
                 420 
               
               
                 LRVRLYRAEG LPALRLGLLG SLVRALHDQR VLVEPYVRVS FLGQEGETSV SAEAAAPEWN 
                 480 
               
               
                 EQLSFVELFP PLTRSLRLQL RDDAPLVDAA LATHVPDLRR ISHPGRAAGF NPTFGPAWVP 
                 540 
               
               
                 LYGSPPGAGL RDSLQGLNEG VGQGIWFRGR LLLAVSMQVL EGRAEPEPPQ AQQGSTLSRL 
                 600 
               
               
                 TRKKKKKARR DQTPKAVPQH LDASPGAEGP EIPRAMEVEV EELLPLPENV LAPCEDFLLF 
                 660 
               
               
                 GVLFEATMID PTVASQPISF EISIGRAGRL EEQLGRGSRA GEGTEGAAVE AQPLLGARPE 
                 720 
               
               
                 EEKEEEELGT HAQRPEPMDG SGPYFCLPLC HCKPCMHVWS CWEDHTWRLQ SSNCVRKVAE 
                 780 
               
               
                 RLDQGLQEVE RLQRKPGPGA CAQLKQALEV LVAGSRQFCH GAERRTMTRP NALDRCRGKL 
                 840 
               
               
                 LVHSLNLLAK QGLRLLRGLR RRNVQKKVAL AKKLLAKLRF LAEEPQPPLP DVLVWMLSGQ 
                 900 
               
               
                 RRVAWARIPA QDVLFSVVEE ERGRDCGKIQ SLMLTAPGAA PGEVCAKLEL FLRLGLGKQA 
                 960 
               
               
                 KACTSELPPD LLPEPSAGLP SSLHRDDFSY FQLRAHLYQA RGVLAADDSG LSDPFARVLI 
                 1020 
               
               
                 STQCQTTRVL EQTLSPLWDE LLVFEQLIVD GRREHLQEEP PLVIINVFDH NKFGPPVFLG 
                 1080 
               
               
                 RALAAPRVKL MEDPYQRPEL QFFPLRKGPW AAGELIAAFQ LIELDYSGRL EPSVPSEVEP 
                 1140 
               
               
                 QDLAPLVEPH SGRLSLPPNV CPVLREFRVE VLFWGLRGLG RVHLLEVEQP QVVLEVAGQG 
                 1200 
               
               
                 VESEVLASYR ESPNFTELVR HLTVVFKDTA PLFHPQDLPE QPYLQPPLSI LVIERRAFGH 
                 1260 
               
               
                 TVLVGSHIVP HMLRFTFRGH EDPPEEEGEM EETGDMMPKG PQGQKSLDPF LAEAGISRQL 
                 1320 
               
               
                 LKPPLKKLPL GGLLNQGPGL EEDIPDPEEL DWGSKYYASL QELQGQHNFD EDEMDDPGDS 
                 1380 
               
               
                 DGVNLISMVG EIQDQGEAEV KGTVSPKKAV ATLKIYNRSL KEEFNHFEDW LNVFPLYRGQ 
                 1440 
               
               
                 GGQDGGGEEE GSGHLVGKFK GSFLIYPESE AVLFSEPQIS RGIPQNRPIK LLVRVYVVKA 
                 1500 
               
               
                 TNLAPADPNG KADPYVVVSA GRERQDTKER YIPKQLNPIF GEILELSISL PAETELTVAV 
                 1560 
               
               
                 FDHDLVGSDD LIGETHIDLE NRFYSHHRAN CGLASQYEVD GYNAWRDAFW PSQILAGLCQ 
                 1620 
               
               
                 RCGLPAPEYR AGAVKVGSKV FLTPPETLPP GSSSPTVASG DPEEAQALLV LRRWQEMPGF 
                 1680 
               
               
                 GIQLVPEHVE TRPLYHPHSP GLLQGSLHMW IDIFPQDVPA PPPVDIKPRQ PISYELRVVI 
                 1740 
               
               
                 WNTEDVVLDD ENPLTGEMSS DIYVKSWVKG LEHDKQETDV HFNSLTGEGN FNWRFVFRFD 
                 1800 
               
               
                 YLPTEREVSV WRRSGPFALE EAEFRQPAVL VLQVWDYDRI SANDFLGSLE LQLPDMVRGA 
                 1860 
               
               
                 RGPELCSVQL ARNGAGPRCN LFRCRRLRGW WPVVKLKEAE DVEREAQEAQ AGKKKRKQRR 
                 1920 
               
               
                 RKGRPEDLEF TDMGGNVYIL TGKVEAEFEL LTVEEAEKRP VGKGRKQPEP LEKPSRPKTS 
                 1980 
               
               
                 FNWFVNPLKT FVFFIWRRYW RTLVLLLLVL LTVFLLLVFY TIPGQISQVI FRPLHK 
                 2036 
               
               
                   
               
               
                 v.18 ORF:2932-4764 Frame +1 610 aa 
                   
               
               
                 Part A 
                   
               
               
                 9-mers 
                   
               
               
                 MCKRRWHWPRSSWQNCAFWLRRHPGQPLVRSVPSWSSSCGWAWASKPRPAPLSCPRICCP 
                   
               
               
                 SPQPGCPPAYTGTVLEQTLSP (SEQ ID NO: 326) 
                   
               
               
                 10-mers 
                   
               
               
                 MCKRRWHWPRSSWQNCAFWLRRHPGQPLVRSVPSWSSSCGWAWASKPRPAPLSCPRICCP 
                   
               
               
                 SPQPGCPPAYTGTVLEQTLSPL (SEQ ID NO: 327) 
                   
               
               
                 15-mers 
                   
               
               
                 MCKRRWHWPRSSWQNCAFWLRRHPGQPLVRSVPSWSSSCGWAWASKPRPAPLSCPRICCP 
                   
               
               
                 SPQPGCPPAYTGTVLEQTLSPLWDELL (SEQ ID NO: 328) 
                   
               
               
                   
               
               
                 Part B 
                   
               
               
                 9-mers 
                   
               
               
                 GISRQLLKHNFDEDEM (SEQ ID NO: 329) 
                   
               
               
                 10-mers 
                   
               
               
                 AGISRQLLKHNFDEDEMD (SEQ ID NO: 330) 
                   
               
               
                 15-mers 
                   
               
               
                 PFLAEAGISRQLLKHNFDEDEMDDPGDS (SEQ ID NO: 331) 
                   
               
               
                   
               
               
                 Part C 
                   
               
               
                 9-mers 
                   
               
               
                 GLASQYEVWVQQGPQEPF (SEQ ID NO: 332) 
                   
               
               
                 10-mers 
                   
               
               
                 CGLASQYEVWVQQGPQEPF (SEQ ID NO: 333) 
                   
               
               
                 15-mers 
                   
               
               
                 HHRANCGLASQYEVWVQQGPQEPF (SEQ ID NO: 334) 
               
               
                   
               
            
           
         
       
     
     Tables VIII-XXI 
     
       
         
           
               
             
               
                 TABLE VIII 
               
             
            
               
                   
               
               
                 158P3D2v.1-A1-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position for  
               
               
                 each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 222 
                 FTDMGGNVY 
                 62.500 
               
               
                   
               
               
                 47 
                 TGEMSSDIY 
                 11.250 
               
               
                   
               
               
                 219 
                 DLEFTDMGG 
                 4.500 
               
               
                   
               
               
                 110 
                 ALEEAEFRQ 
                 4.500 
               
               
                   
               
               
                 237 
                 EAEFELLTV 
                 4.500 
               
               
                   
               
               
                 247 
                 EAEKRPVGK 
                 3.600 
               
               
                   
               
               
                 198 
                 AQEAQAGKK 
                 2.700 
               
               
                   
               
               
                 78 
                 TGEGNFNWR 
                 2.250 
               
               
                   
               
               
                 259 
                 QPEPLEKPS 
                 2.250 
               
               
                   
               
               
                 113 
                 EAEFRQPAV 
                 1.800 
               
               
                   
               
               
                 140 
                 SLELQLPDM 
                 1.800 
               
               
                   
               
               
                 281 
                 KTFVFFIWR 
                 1.250 
               
               
                   
               
               
                 303 
                 LTVFLLLVF 
                 1.250 
               
               
                   
               
               
                 145 
                 LPDMVRGAR 
                 1.250 
               
               
                   
               
               
                 312 
                 YTIPGQISQ 
                 1.250 
               
               
                   
               
               
                 69 
                 ETDVHFNSL 
                 1.250 
               
               
                   
               
               
                 34 
                 NTEDVVLDD 
                 1.125 
               
               
                   
               
               
                 320 
                 QVIFRPLHK 
                 1.000 
               
               
                   
               
               
                 166 
                 GAGPRCNLF 
                 1.000 
               
               
                   
               
               
                 304 
                 TVFLLLVFY 
                 1.000 
               
               
                   
               
               
                 39 
                 VLDDENPLT 
                 1.000 
               
               
                   
               
               
                 188 
                 LKEAEDVER 
                 0.900 
               
               
                   
               
               
                 235 
                 KVEAEFELL 
                 0.900 
               
               
                   
               
               
                 190 
                 EAEDVEREA 
                 0.900 
               
               
                   
               
               
                 62 
                 GLEHDKQET 
                 0.900 
               
               
                   
               
               
                 51 
                 SSDIYVKSW 
                 0.750 
               
               
                   
               
               
                 2 
                 WIDIFPQDV 
                 0.500 
               
               
                   
               
               
                 257 
                 RKQPEPLEK 
                 0.500 
               
               
                   
               
               
                 142 
                 ELQLPDMVR 
                 0.500 
               
               
                   
               
               
                 283 
                 FVFFIWRRY 
                 0.500 
               
               
                   
               
               
                 121 
                 VLVLQVWDY 
                 0.500 
               
               
                   
               
               
                 156 
                 ELCSVQLAR 
                 0.500 
               
               
                   
               
               
                 154 
                 GPELCSVQL 
                 0.450 
               
               
                   
               
               
                 97 
                 EREVSVWRR 
                 0.450 
               
               
                   
               
               
                 242 
                 LLTVEEAEK 
                 0.400 
               
               
                   
               
               
                 197 
                 EAQEAQAGK 
                 0.400 
               
               
                   
               
               
                 243 
                 LTVEEAEKR 
                 0.250 
               
               
                   
               
               
                 90 
                 RFDYLPTER 
                 0.250 
               
               
                   
               
               
                 49 
                 EMSSDIYVK 
                 0.200 
               
               
                   
               
               
                 4 
                 DIFPQDVPA 
                 0.200 
               
               
                   
               
               
                 11 
                 PAPPPVDIK 
                 0.200 
               
               
                   
               
               
                 123 
                 VLQVWDYDR 
                 0.200 
               
               
                   
               
               
                 53 
                 DIYVKSWVK 
                 0.200 
               
               
                   
               
               
                 262 
                 PLEKPSRPK 
                 0.180 
               
               
                   
               
               
                 75 
                 NSLTGEGNF 
                 0.150 
               
               
                   
               
               
                 67 
                 KQETDVHFN 
                 0.135 
               
               
                   
               
               
                 126 
                 VWDYDRISA 
                 0.125 
               
               
                   
               
               
                 293 
                 RTLVLLLLV 
                 0.125 
               
               
                   
               
               
                 81 
                 GNFNWRFVF 
                 0.125 
               
               
                   
               
               
                 277 
                 VNPLKTFVF 
                 0.125 
               
               
                   
               
               
                 77 
                 LTGEGNFNW 
                 0.125 
               
               
                   
               
               
                 214 
                 KGRPEDLEF 
                 0.125 
               
               
                   
               
               
                 270 
                 KTSFNWFVN 
                 0.125 
               
               
                   
               
               
                 85 
                 WRFVFRFDY 
                 0.125 
               
               
                   
               
               
                 40 
                 LDDENPLTG 
                 0.125 
               
               
                   
               
               
                 216 
                 RPEDLEFTD 
                 0.113 
               
               
                   
               
               
                 298 
                 LLLVLLTVF 
                 0.100 
               
               
                   
               
               
                 200 
                 EAQAGKKKR 
                 0.100 
               
               
                   
               
               
                 170 
                 RCNLFRCRR 
                 0.100 
               
               
                   
               
               
                 109 
                 FALEEAEFR 
                 0.100 
               
               
                   
               
               
                 276 
                 FVNPLKTFV 
                 0.100 
               
               
                   
               
               
                 244 
                 TVEEAEKRP 
                 0.090 
               
               
                   
               
               
                 25 
                 SYELRVVIW 
                 0.090 
               
               
                   
               
               
                 193 
                 DVEREAQEA 
                 0.090 
               
               
                   
               
               
                 195 
                 EREAQEAQA 
                 0.090 
               
               
                   
               
               
                 132 
                 ISANDFLGS 
                 0.075 
               
               
                   
               
               
                 316 
                 GQISQVIFR 
                 0.075 
               
               
                   
               
               
                 105 
                 RSGPFALEE 
                 0.075 
               
               
                   
               
               
                 10 
                 VPAPPPVDI 
                 0.050 
               
               
                   
               
               
                 71 
                 DVHFNSLTG 
                 0.050 
               
               
                   
               
               
                 300 
                 LVLLTVFLL 
                 0.050 
               
               
                   
               
               
                 137 
                 FLGSLELQL 
                 0.050 
               
               
                   
               
               
                 232 
                 LTGKVEAEF 
                 0.050 
               
               
                   
               
               
                 294 
                 TLVLLLLVL 
                 0.050 
               
               
                   
               
               
                 301 
                 VLLTVFLLL 
                 0.050 
               
               
                   
               
               
                 302 
                 LLTVFLLLV 
                 0.050 
               
               
                   
               
               
                 227 
                 GNVYILTGK 
                 0.050 
               
               
                   
               
               
                 297 
                 LLLLVLLTV 
                 0.050 
               
               
                   
               
               
                 296 
                 VLLLLVLLT 
                 0.050 
               
               
                   
               
               
                 131 
                 RISANDFLG 
                 0.050 
               
               
                   
               
               
                 308 
                 LLVFYTIPG 
                 0.050 
               
               
                   
               
               
                 245 
                 VEEAEKRPV 
                 0.045 
               
               
                   
               
               
                 143 
                 LQLPDMVRG 
                 0.030 
               
               
                   
               
               
                 24 
                 ISYELRVVI 
                 0.030 
               
               
                   
               
               
                 201 
                 AQAGKKKRK 
                 0.030 
               
               
                   
               
               
                 50 
                 MSSDIYVKS 
                 0.030 
               
               
                   
               
               
                 116 
                 FRQPAVLVL 
                 0.025 
               
               
                   
               
               
                 46 
                 LTGEMSSDI 
                 0.025 
               
               
                   
               
               
                 191 
                 AEDVEREAQ 
                 0.025 
               
               
                   
               
               
                 95 
                 PTEREVSVW 
                 0.022 
               
               
                   
               
               
                 59 
                 WVKGLEHDK 
                 0.020 
               
               
                   
               
               
                 179 
                 LRGWWPVVK 
                 0.020 
               
               
                   
               
               
                 306 
                 FLLLVFYTI 
                 0.020 
               
               
                   
               
               
                 157 
                 LCSVQLARN 
                 0.020 
               
               
                   
               
               
                 230 
                 YILTGKVEA 
                 0.020 
               
               
                   
               
               
                 309 
                 LVFYTIPGQ 
                 0.020 
               
               
                   
               
               
                 299 
                 LLVLLTVFL 
                 0.020 
               
               
                   
               
               
                 17 
                 DIKPRQPIS 
                 0.020 
               
               
                   
               
               
                 295 
                 LVLLLLVLL 
                 0.020 
               
               
                   
               
               
                 158 
                 CSVQLARNG 
                 0.015 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IX 
               
             
            
               
                   
               
               
                 158P3D2v.1-A1-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 259 
                 QPEPlEKPSR 
                 45.000 
               
               
                   
               
               
                 276 
                 FVNPlKTFVF 
                 5.000 
               
               
                   
               
               
                 166 
                 GAGPrCNLFR 
                 5.000 
               
               
                   
               
               
                 235 
                 KVEAeFELLT 
                 4.500 
               
               
                   
               
               
                 198 
                 AQEAqAGKKK 
                 2.700 
               
               
                   
               
               
                 39 
                 VLDDeNPLTG 
                 2.500 
               
               
                   
               
               
                 303 
                 LTVFlLLVFY 
                 2.500 
               
               
                   
               
               
                 17 
                 DIKPrQPISY 
                 2.500 
               
               
                   
               
               
                 222 
                 FTDMgGNVYI 
                 2.500 
               
               
                   
               
               
                 78 
                 TGEGnFNWRF 
                 2.250 
               
               
                   
               
               
                 113 
                 EAEFrQPAVL 
                 1.800 
               
               
                   
               
               
                 46 
                 LTGEmSSDIY 
                 1.250 
               
               
                   
               
               
                 69 
                 ETDVhFNSLT 
                 1.250 
               
               
                   
               
               
                 47 
                 TGEMsSDIYV 
                 1.125 
               
               
                   
               
               
                 140 
                 SLELqLPDMV 
                 0.900 
               
               
                   
               
               
                 219 
                 DLEFtDMGGN 
                 0.900 
               
               
                   
               
               
                 190 
                 EAEDvEREAQ 
                 0.900 
               
               
                   
               
               
                 244 
                 TVEEaEKRPV 
                 0.900 
               
               
                   
               
               
                 51 
                 SSDIyVKSWV 
                 0.750 
               
               
                   
               
               
                 67 
                 KQETdVHFNS 
                 0.675 
               
               
                   
               
               
                 134 
                 ANDFlGSLEL 
                 0.625 
               
               
                   
               
               
                 120 
                 AVLVlQVWDY 
                 0.500 
               
               
                   
               
               
                 302 
                 LLTVfLLLVF 
                 0.500 
               
               
                   
               
               
                 10 
                 VPAPpPVDIK 
                 0.500 
               
               
                   
               
               
                 95 
                 PTEReVSVWR 
                 0.450 
               
               
                   
               
               
                 241 
                 ELLTvEEAEK 
                 0.400 
               
               
                   
               
               
                 312 
                 YTIPgQISQV 
                 0.250 
               
               
                   
               
               
                 281 
                 KTFVfFIWRR 
                 0.250 
               
               
                   
               
               
                 145 
                 LPDMvRGARG 
                 0.250 
               
               
                   
               
               
                 77 
                 LTGEgNFNWR 
                 0.250 
               
               
                   
               
               
                 12 
                 APPPvDIKPR 
                 0.250 
               
               
                   
               
               
                 154 
                 GPELcSVQLA 
                 0.225 
               
               
                   
               
               
                 216 
                 RPEDlEFTDM 
                 0.225 
               
               
                   
               
               
                 34 
                 NTEDvVLDDE 
                 0.225 
               
               
                   
               
               
                 25 
                 SYELrVVIWN 
                 0.225 
               
               
                   
               
               
                 122 
                 LVLQvWDYDR 
                 0.200 
               
               
                   
               
               
                 231 
                 ILTGkVEAEF 
                 0.200 
               
               
                   
               
               
                 197 
                 EAQEaQAGKK 
                 0.200 
               
               
                   
               
               
                 200 
                 EAQAgKKKRK 
                 0.200 
               
               
                   
               
               
                 100 
                 VSVWrRSGPF 
                 0.150 
               
               
                   
               
               
                 105 
                 RSGPfALEEA 
                 0.150 
               
               
                   
               
               
                 319 
                 SQVIfRPLHK 
                 0.150 
               
               
                   
               
               
                 80 
                 EGNFnWRFVF 
                 0.125 
               
               
                   
               
               
                 293 
                 RTLVlLLLVL 
                 0.125 
               
               
                   
               
               
                 297 
                 LLLLvLLTVF 
                 0.100 
               
               
                   
               
               
                 144 
                 QLPDmVRGAR 
                 0.100 
               
               
                   
               
               
                 242 
                 LLTVeEAEKR 
                 0.100 
               
               
                   
               
               
                 193 
                 DVEReAQEAQ 
                 0.090 
               
               
                   
               
               
                 247 
                 EAEKrPVGKG 
                 0.090 
               
               
                   
               
               
                 62 
                 GLEHdKQETD 
                 0.090 
               
               
                   
               
               
                 245 
                 VEEAeKRPVG 
                 0.090 
               
               
                   
               
               
                 110 
                 ALEEaEFRQP 
                 0.090 
               
               
                   
               
               
                 237 
                 EAEFeLLTVE 
                 0.090 
               
               
                   
               
               
                 107 
                 GPFAlEEAEF 
                 0.050 
               
               
                   
               
               
                 15 
                 PVDIkPRQPI 
                 0.050 
               
               
                   
               
               
                 304 
                 TVFLlLVFYT 
                 0.050 
               
               
                   
               
               
                 2 
                 WIDIfPQDVP 
                 0.050 
               
               
                   
               
               
                 76 
                 SLTGeGNFNW 
                 0.050 
               
               
                   
               
               
                 307 
                 LLLVfYTIPG 
                 0.050 
               
               
                   
               
               
                 300 
                 LVLLtVFLLL 
                 0.050 
               
               
                   
               
               
                 295 
                 LVLLlLVLLT 
                 0.050 
               
               
                   
               
               
                 301 
                 VLLTvFLLLV 
                 0.050 
               
               
                   
               
               
                 299 
                 LLVLlTVFLL 
                 0.050 
               
               
                   
               
               
                 261 
                 EPLEkPSRPK 
                 0.050 
               
               
                   
               
               
                 277 
                 VNPLkTFVFF 
                 0.050 
               
               
                   
               
               
                 109 
                 FALEeAEFRQ 
                 0.050 
               
               
                   
               
               
                 81 
                 GNFNwRFVFR 
                 0.050 
               
               
                   
               
               
                 296 
                 VLLLlVLLTV 
                 0.050 
               
               
                   
               
               
                 314 
                 IPGQiSQVIF 
                 0.050 
               
               
                   
               
               
                 226 
                 GGNVyILTGK 
                 0.050 
               
               
                   
               
               
                 131 
                 RISAnDFLGS 
                 0.050 
               
               
                   
               
               
                 97 
                 EREVsVWRRS 
                 0.045 
               
               
                   
               
               
                 239 
                 EFELlTVEEA 
                 0.045 
               
               
                   
               
               
                 111 
                 LEEAeFRQPA 
                 0.045 
               
               
                   
               
               
                 41 
                 DDENpLTGEM 
                 0.045 
               
               
                   
               
               
                 195 
                 EREAqEAQAG 
                 0.045 
               
               
                   
               
               
                 178 
                 RLRGwWPVVK 
                 0.040 
               
               
                   
               
               
                 24 
                 ISYElRVVIW 
                 0.030 
               
               
                   
               
               
                 139 
                 GSLElQLPDM 
                 0.030 
               
               
                   
               
               
                 318 
                 ISQViFRPLH 
                 0.030 
               
               
                   
               
               
                 224 
                 DMGGnVYILT 
                 0.025 
               
               
                   
               
               
                 165 
                 NGAGpRCNLF 
                 0.025 
               
               
                   
               
               
                 282 
                 TFVFfIWRRY 
                 0.025 
               
               
                   
               
               
                 280 
                 LKTFvFFIWR 
                 0.025 
               
               
                   
               
               
                 82 
                 NFNWrFVFRF 
                 0.025 
               
               
                   
               
               
                 171 
                 CNLFrCRRLR 
                 0.025 
               
               
                   
               
               
                 126 
                 VWDYdRISAN 
                 0.025 
               
               
                   
               
               
                 128 
                 DYDRiSANDF 
                 0.025 
               
               
                   
               
               
                 141 
                 LELQlPDMVR 
                 0.025 
               
               
                   
               
               
                 35 
                 TEDVvLDDEN 
                 0.025 
               
               
                   
               
               
                 74 
                 FNSLtGEGNF 
                 0.025 
               
               
                   
               
               
                 221 
                 EFTDmGGNVY 
                 0.025 
               
               
                   
               
               
                 294 
                 TLVLlLLVLL 
                 0.020 
               
               
                   
               
               
                 38 
                 VVLDdENPLT 
                 0.020 
               
               
                   
               
               
                 142 
                 ELQLpDMVRG 
                 0.020 
               
               
                   
               
               
                 53 
                 DIYVkSWVKG 
                 0.020 
               
               
                   
               
               
                 246 
                 EEAEkRPVGK 
                 0.020 
               
               
                   
               
               
                 187 
                 KLKEaEDVER 
                 0.020 
               
               
                   
               
               
                 272 
                 SFNWfVNPLK 
                 0.020 
               
               
                   
               
               
                 298 
                 LLLVlLTVFL 
                 0.020 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE X 
               
             
            
               
                   
               
               
                 158P3D2v.1-A0201-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 302 
                 LLTVFLLLV 
                 1033.404 
               
               
                 297 
                 LLLLVLLTV 
                 1006.209 
               
               
                 286 
                 FIWRRYWRT 
                 440.113 
               
               
                 306 
                 FLLLVFYTI 
                 337.376 
               
               
                 301 
                 VLLTVFLLL 
                 255.302 
               
               
                 299 
                 LLVLLTVFL 
                 199.738 
               
               
                 300 
                 LVLLTVFLL 
                 156.843 
               
               
                 276 
                 FVNPLKTFV 
                 153.971 
               
               
                 296 
                 VLLLLVLLT 
                 107.808 
               
               
                 137 
                 FLGSLELQL 
                 98.267 
               
               
                 2 
                 WIDIFPQDV 
                 66.867 
               
               
                 38 
                 VVLDDENPL 
                 48.205 
               
               
                 48 
                 GEMSSDIYV 
                 27.521 
               
               
                 31 
                 VIWNTEDVV 
                 27.109 
               
               
                 295 
                 LVLLLLVLL 
                 27.042 
               
               
                 313 
                 TIPGQISQV 
                 21.996 
               
               
                 39 
                 VLDDENPLT 
                 20.776 
               
               
                 294 
                 TLVLLLLVL 
                 20.145 
               
               
                 230 
                 YILTGKVEA 
                 11.626 
               
               
                 144 
                 QLPDMVRGA 
                 9.370 
               
               
                 293 
                 RTLVLLLLV 
                 8.221 
               
               
                 30 
                 VVIWNTEDV 
                 5.069 
               
               
                 141 
                 LELQLPDMV 
                 4.168 
               
               
                 236 
                 VEAEFELLT 
                 3.838 
               
               
                 178 
                 RLRGWWPVV 
                 3.684 
               
               
                 94 
                 LPTEREVSV 
                 3.165 
               
               
                 180 
                 RGWWPVVKL 
                 2.662 
               
               
                 228 
                 NVYILTGKV 
                 2.532 
               
               
                 305 
                 VFLLLVFYT 
                 2.388 
               
               
                 279 
                 PLKTFVFFI 
                 2.240 
               
               
                 121 
                 VLVLQVWDY 
                 2.185 
               
               
                 240 
                 FELLTVEEA 
                 1.853 
               
               
                 133 
                 SANDFLGSL 
                 1.382 
               
               
                 124 
                 LQVWDYDRI 
                 1.322 
               
               
                 224 
                 DMGGNVYIL 
                 1.091 
               
               
                 118 
                 QPAVLVLQV 
                 1.044 
               
               
                 46 
                 LTGEMSSDI 
                 1.010 
               
               
                 83 
                 FNWRFVFRF 
                 0.941 
               
               
                 27 
                 ELRVVIWNT 
                 0.733 
               
               
                 140 
                 SLELQLPDM 
                 0.731 
               
               
                 234 
                 GKVEAEFEL 
                 0.706 
               
               
                 55 
                 YVKSWVKGL 
                 0.692 
               
               
                 114 
                 AEFRQPAVL 
                 0.630 
               
               
                 24 
                 ISYELRVVI 
                 0.623 
               
               
                 52 
                 SDIYVKSWV 
                 0.531 
               
               
                 62 
                 GLEHDKQET 
                 0.477 
               
               
                 177 
                 RRLRGWWPV 
                 0.456 
               
               
                 22 
                 QPISYELRV 
                 0.454 
               
               
                 298 
                 LLLVLLTVF 
                 0.442 
               
               
                 159 
                 SVQLARNGA 
                 0.435 
               
               
                 76 
                 SLTGEGNFN 
                 0.410 
               
               
                 235 
                 KVEAEFELL 
                 0.390 
               
               
                 183 
                 WPVVKLKEA 
                 0.343 
               
               
                 269 
                 PKTSFNWFV 
                 0.333 
               
               
                 26 
                 YELRVVIWN 
                 0.312 
               
               
                 304 
                 TVFLLLVFY 
                 0.305 
               
               
                 186 
                 VKLKEAEDV 
                 0.298 
               
               
                 223 
                 TDMGGNVYI 
                 0.295 
               
               
                 307 
                 LLLVFYTIP 
                 0.219 
               
               
                 4 
                 DIFPQDVPA 
                 0.190 
               
               
                 165 
                 NGAGPRCNL 
                 0.139 
               
               
                 272 
                 SFNWFVNPL 
                 0.130 
               
               
                 308 
                 LLVFYTIPG 
                 0.127 
               
               
                 225 
                 MGGNVYILT 
                 0.124 
               
               
                 10 
                 VPAPPPVDI 
                 0.116 
               
               
                 112 
                 EEAEFRQPA 
                 0.113 
               
               
                 135 
                 NDFLGSLEL 
                 0.110 
               
               
                 143 
                 LQLPDMVRG 
                 0.109 
               
               
                 281 
                 KTFVFFIWR 
                 0.106 
               
               
                 171 
                 CNLFRCRRL 
                 0.103 
               
               
                 8 
                 QDVPAPPPV 
                 0.097 
               
               
                 318 
                 ISQVIFRPL 
                 0.090 
               
               
                 87 
                 FVFRFDYLP 
                 0.084 
               
               
                 86 
                 RFVFRFDYL 
                 0.076 
               
               
                 93 
                 YLPTEREVS 
                 0.069 
               
               
                 80 
                 EGNFNWRFV 
                 0.064 
               
               
                 131 
                 RISANDFLG 
                 0.059 
               
               
                 290 
                 RYWRTLVLL 
                 0.057 
               
               
                 314 
                 IPGQISQVI 
                 0.047 
               
               
                 77 
                 LTGEGNFNW 
                 0.042 
               
               
                 79 
                 GEGNFNWRF 
                 0.041 
               
               
                 23 
                 PISYELRVV 
                 0.040 
               
               
                 70 
                 TDVHFNSLT 
                 0.039 
               
               
                 109 
                 FALEEAEFR 
                 0.039 
               
               
                 283 
                 FVFFIWRRY 
                 0.038 
               
               
                 122 
                 LVLQVWDYD 
                 0.038 
               
               
                 106 
                 SGPFALEEA 
                 0.037 
               
               
                 68 
                 QETDVHFNS 
                 0.034 
               
               
                 168 
                 GPRCNLFRC 
                 0.033 
               
               
                 292 
                 WRTLVLLLL 
                 0.031 
               
               
                 245 
                 VEEAEKRPV 
                 0.029 
               
               
                 319 
                 SQVIFRPLH 
                 0.029 
               
               
                 231 
                 ILTGKVEAE 
                 0.029 
               
               
                 317 
                 QISQVIFRP 
                 0.027 
               
               
                 120 
                 AVLVLQVWD 
                 0.027 
               
               
                 215 
                 GRPEDLEFT 
                 0.026 
               
               
                 242 
                 LLTVEEAEK 
                 0.025 
               
               
                 123 
                 VLQVWDYDR 
                 0.025 
               
               
                 16 
                 VDIKPRQPI 
                 0.025 
               
               
                 258 
                 KQPEPLEKP 
                 0.024 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XI 
               
             
            
               
                   
               
               
                 158P3D2v.1-A0201-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 301 
                 VLLTvFLLLV 
                 3823.593 
               
               
                 296 
                 VLLLlVLLTV 
                 1006.209 
               
               
                 298 
                 LLLVlLTVFL 
                 739.032 
               
               
                 299 
                 LLVLlTVFLL 
                 484.457 
               
               
                 93 
                 YLPTeREVSV 
                 319.939 
               
               
                 304 
                 TVFLlLVFYT 
                 177.011 
               
               
                 278 
                 NPLKtFVFFI 
                 70.254 
               
               
                 294 
                 TLVLlLLVLL 
                 49.134 
               
               
                 26 
                 YELRvVIWNT 
                 42.542 
               
               
                 286 
                 FIWRrYWRTL 
                 38.130 
               
               
                 300 
                 LVLLtVFLLL 
                 22.339 
               
               
                 236 
                 VEAEfELLTV 
                 21.680 
               
               
                 101 
                 SVWRrSGPFA 
                 19.844 
               
               
                 31 
                 VIWNtEDVVL 
                 16.993 
               
               
                 38 
                 VVLDdENPLT 
                 16.816 
               
               
                 87 
                 FVFRfDYLPT 
                 16.647 
               
               
                 117 
                 RQPAvLVLQV 
                 16.219 
               
               
                 125 
                 QVWDyDRISA 
                 14.793 
               
               
                 123 
                 VLQVwDYDRI 
                 13.036 
               
               
                 312 
                 YTIPgQISQV 
                 10.220 
               
               
                 295 
                 LVLLlLVLLT 
                 9.433 
               
               
                 63 
                 LEHDkQETDV 
                 9.426 
               
               
                 21 
                 RQPIsYELRV 
                 7.052 
               
               
                 114 
                 AEFRqPAVLV 
                 5.004 
               
               
                 271 
                 TSFNwFVNPL 
                 4.510 
               
               
                 68 
                 QETDvHFNSL 
                 3.236 
               
               
                 29 
                 RVVIwNTEDV 
                 2.982 
               
               
                 61 
                 KGLEhDKQET 
                 2.583 
               
               
                 79 
                 GEGNfNWRFV 
                 2.529 
               
               
                 268 
                 RPKTsFNWFV 
                 2.491 
               
               
                 140 
                 SLELqLPDMV 
                 2.181 
               
               
                 30 
                 VVIWnTEDVV 
                 2.078 
               
               
                 273 
                 FNWFvNPLKT 
                 1.857 
               
               
                 222 
                 FTDMgGNVYI 
                 1.466 
               
               
                 143 
                 LQLPdMVRGA 
                 1.457 
               
               
                 275 
                 WFVNpLKTFV 
                 1.222 
               
               
                 139 
                 GSLElQLPDM 
                 1.132 
               
               
                 317 
                 QISQvIFRPL 
                 1.116 
               
               
                 220 
                 LEFTdMGGNV 
                 1.106 
               
               
                 293 
                 RTLVlLLLVL 
                 1.035 
               
               
                 51 
                 SSDIyVKSWV 
                 0.999 
               
               
                 309 
                 LVFYtIPGQI 
                 0.746 
               
               
                 224 
                 DMGGnVYILT 
                 0.605 
               
               
                 306 
                 FLLLvFYTIP 
                 0.593 
               
               
                 313 
                 TIPGqISQVI 
                 0.588 
               
               
                 153 
                 RGPElCSVQL 
                 0.572 
               
               
                 235 
                 KVEAeFELLT 
                 0.555 
               
               
                 307 
                 LLLVfYTIPG 
                 0.469 
               
               
                 297 
                 LLLLvLLTVF 
                 0.442 
               
               
                 167 
                 AGPRcNLFRC 
                 0.433 
               
               
                 76 
                 SLTGeGNFNW 
                 0.432 
               
               
                 120 
                 AVLVlQVWDY 
                 0.416 
               
               
                 112 
                 EEAEfRQPAV 
                 0.416 
               
               
                 244 
                 TVEEaEKRPV 
                 0.319 
               
               
                 91 
                 FDYLpTEREV 
                 0.284 
               
               
                 189 
                 KEAEdVEREA 
                 0.277 
               
               
                 172 
                 NLFRcRRLRG 
                 0.276 
               
               
                 132 
                 ISANdFLGSL 
                 0.269 
               
               
                 285 
                 FFIWrRYWRT 
                 0.268 
               
               
                 85 
                 WRFVfRFDYL 
                 0.259 
               
               
                 1 
                 MWIDiFPQDV 
                 0.256 
               
               
                 148 
                 MVRGaRGPEL 
                 0.242 
               
               
                 45 
                 PLTGeMSSDI 
                 0.230 
               
               
                 39 
                 VLDDeNPLTG 
                 0.208 
               
               
                 185 
                 VVKLkEAEDV 
                 0.177 
               
               
                 281 
                 KTFVfFIWRR 
                 0.176 
               
               
                 151 
                 GARGpELCSV 
                 0.169 
               
               
                 47 
                 TGEMsSDIYV 
                 0.160 
               
               
                 137 
                 FLGSlELQLP 
                 0.158 
               
               
                 37 
                 DVVLdDENPL 
                 0.140 
               
               
                 164 
                 RNGAgPRCNL 
                 0.139 
               
               
                 231 
                 ILTGkVEAEF 
                 0.127 
               
               
                 283 
                 FVFFiWRRYW 
                 0.122 
               
               
                 302 
                 LLTVfLLLVF 
                 0.119 
               
               
                 121 
                 VLVLqVWDYD 
                 0.116 
               
               
                 234 
                 GKVEaEFELL 
                 0.113 
               
               
                 258 
                 KQPEpLEKPS 
                 0.108 
               
               
                 223 
                 TDMGgNVYIL 
                 0.104 
               
               
                 292 
                 WRTLvLLLLV 
                 0.102 
               
               
                 305 
                 VFLLlVFYTI 
                 0.087 
               
               
                 22 
                 QPISyELRVV 
                 0.086 
               
               
                 109 
                 FALEeAEFRQ 
                 0.084 
               
               
                 214 
                 KGRPeDLEFT 
                 0.080 
               
               
                 276 
                 FVNPlKTFVF 
                 0.071 
               
               
                 9 
                 DVPApPPVDI 
                 0.068 
               
               
                 7 
                 PQDVpAPPPV 
                 0.062 
               
               
                 227 
                 GNVYiLTGKV 
                 0.059 
               
               
                 308 
                 LLVFyTIPGQ 
                 0.058 
               
               
                 290 
                 RYWRtLVLLL 
                 0.057 
               
               
                 134 
                 ANDFlGSLEL 
                 0.056 
               
               
                 194 
                 VEREaQEAQA 
                 0.051 
               
               
                 111 
                 LEEAeFRQPA 
                 0.040 
               
               
                 230 
                 YILTgKVEAE 
                 0.039 
               
               
                 19 
                 KPRQpISYEL 
                 0.037 
               
               
                 105 
                 RSGPfALEEA 
                 0.037 
               
               
                 158 
                 CSVQlARNGA 
                 0.032 
               
               
                 233 
                 TGKVeAEFEL 
                 0.028 
               
               
                 129 
                 YDRIsANDFL 
                 0.028 
               
               
                 170 
                 RCNLfRCRRL 
                 0.028 
               
               
                 177 
                 RRLRgWWPVV 
                 0.025 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XII 
               
             
            
               
                   
               
               
                 158P3D2v.1-A3-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 281 
                 KTFVFFIWR 
                 54.000 
               
               
                 121 
                 VLVLQVWDY 
                 54.000 
               
               
                 123 
                 VLQVWDYDR 
                 36.000 
               
               
                 49 
                 EMSSDIYVK 
                 27.000 
               
               
                 242 
                 LLTVEEAEK 
                 20.000 
               
               
                 306 
                 FLLLVFYTI 
                 12.150 
               
               
                 53 
                 DIYVKSWVK 
                 9.000 
               
               
                 301 
                 VLLTVFLLL 
                 8.100 
               
               
                 320 
                 QVIFRPLHK 
                 6.000 
               
               
                 298 
                 LLLVLLTVF 
                 4.500 
               
               
                 142 
                 ELQLPDMVR 
                 3.600 
               
               
                 156 
                 ELCSVQLAR 
                 3.600 
               
               
                 316 
                 GQISQVIFR 
                 3.240 
               
               
                 59 
                 WVKGLEHDK 
                 3.000 
               
               
                 304 
                 TVFLLLVFY 
                 3.000 
               
               
                 294 
                 TLVLLLLVL 
                 2.700 
               
               
                 224 
                 DMGGNVYIL 
                 2.430 
               
               
                 172 
                 NLFRCRRLR 
                 2.000 
               
               
                 302 
                 LLTVFLLLV 
                 1.800 
               
               
                 279 
                 PLKTFVFFI 
                 1.620 
               
               
                 297 
                 LLLLVLLTV 
                 1.350 
               
               
                 137 
                 FLGSLELQL 
                 1.200 
               
               
                 181 
                 GWWPVVKLK 
                 1.013 
               
               
                 299 
                 LLVLLTVFL 
                 0.900 
               
               
                 296 
                 VLLLLVLLT 
                 0.900 
               
               
                 178 
                 RLRGWWPVV 
                 0.900 
               
               
                 300 
                 LVLLTVFLL 
                 0.810 
               
               
                 81 
                 GNFNWRFVF 
                 0.540 
               
               
                 235 
                 KVEAEFELL 
                 0.540 
               
               
                 83 
                 FNWRFVFRF 
                 0.540 
               
               
                 303 
                 LTVFLLLVF 
                 0.450 
               
               
                 243 
                 LTVEEAEKR 
                 0.450 
               
               
                 201 
                 AQAGKKKRK 
                 0.450 
               
               
                 227 
                 GNVYILTGK 
                 0.405 
               
               
                 62 
                 GLEHDKQET 
                 0.300 
               
               
                 273 
                 FNWFVNPLK 
                 0.300 
               
               
                 262 
                 PLEKPSRPK 
                 0.300 
               
               
                 283 
                 FVFFIWRRY 
                 0.300 
               
               
                 101 
                 SVWRRSGPF 
                 0.300 
               
               
                 140 
                 SLELQLPDM 
                 0.300 
               
               
                 55 
                 YVKSWVKGL 
                 0.270 
               
               
                 27 
                 ELRVVIWNT 
                 0.203 
               
               
                 222 
                 FTDMGGNVY 
                 0.200 
               
               
                 85 
                 WRFVFRFDY 
                 0.180 
               
               
                 308 
                 LLVFYTIPG 
                 0.180 
               
               
                 198 
                 AQEAQAGKK 
                 0.180 
               
               
                 79 
                 GEGNFNWRF 
                 0.162 
               
               
                 286 
                 FIWRRYWRT 
                 0.150 
               
               
                 232 
                 LTGKVEAEF 
                 0.150 
               
               
                 295 
                 LVLLLLVLL 
                 0.135 
               
               
                 11 
                 PAPPPVDIK 
                 0.135 
               
               
                 21 
                 RQPISYELR 
                 0.120 
               
               
                 170 
                 RCNLFRCRR 
                 0.120 
               
               
                 31 
                 VIWNTEDVV 
                 0.100 
               
               
                 39 
                 VLDDENPLT 
                 0.100 
               
               
                 278 
                 NPLKTFVFF 
                 0.090 
               
               
                 187 
                 KLKEAEDVE 
                 0.090 
               
               
                 231 
                 ILTGKVEAE 
                 0.090 
               
               
                 265 
                 KPSRPKTSF 
                 0.090 
               
               
                 87 
                 FVFRFDYLP 
                 0.090 
               
               
                 110 
                 ALEEAEFRQ 
                 0.090 
               
               
                 307 
                 LLLVFYTIP 
                 0.090 
               
               
                 38 
                 VVLDDENPL 
                 0.090 
               
               
                 166 
                 GAGPRCNLF 
                 0.090 
               
               
                 109 
                 FALEEAEFR 
                 0.090 
               
               
                 197 
                 EAQEAQAGK 
                 0.090 
               
               
                 282 
                 TFVFFIWRR 
                 0.081 
               
               
                 179 
                 LRGWWPVVK 
                 0.060 
               
               
                 257 
                 RKQPEPLEK 
                 0.060 
               
               
                 144 
                 QLPDMVRGA 
                 0.060 
               
               
                 268 
                 RPKTSFNWF 
                 0.060 
               
               
                 247 
                 EAEKRPVGK 
                 0.060 
               
               
                 2 
                 WIDIFPQDV 
                 0.060 
               
               
                 46 
                 LTGEMSSDI 
                 0.045 
               
               
                 293 
                 RTLVLLLLV 
                 0.045 
               
               
                 4 
                 DIFPQDVPA 
                 0.045 
               
               
                 77 
                 LTGEGNFNW 
                 0.045 
               
               
                 313 
                 TIPGQISQV 
                 0.045 
               
               
                 93 
                 YLPTEREVS 
                 0.040 
               
               
                 230 
                 YILTGKVEA 
                 0.030 
               
               
                 76 
                 SLTGEGNFN 
                 0.030 
               
               
                 228 
                 NVYILTGKV 
                 0.030 
               
               
                 57 
                 KSWVKGLEH 
                 0.030 
               
               
                 276 
                 FVNPLKTFV 
                 0.030 
               
               
                 30 
                 VVIWNTEDV 
                 0.030 
               
               
                 199 
                 QEAQAGKKK 
                 0.030 
               
               
                 69 
                 ETDVHFNSL 
                 0.027 
               
               
                 319 
                 SQVIFRPLH 
                 0.027 
               
               
                 168 
                 GPRCNLFRC 
                 0.027 
               
               
                 124 
                 LQVWDYDRI 
                 0.027 
               
               
                 96 
                 TEREVSVWR 
                 0.027 
               
               
                 24 
                 ISYELRVVI 
                 0.022 
               
               
                 159 
                 SVQLARNGA 
                 0.020 
               
               
                 161 
                 QLARNGAGP 
                 0.020 
               
               
                 285 
                 FFIWRRYWR 
                 0.018 
               
               
                 250 
                 KRPVGKGRK 
                 0.018 
               
               
                 214 
                 KGRPEDLEF 
                 0.018 
               
               
                 78 
                 TGEGNFNWR 
                 0.018 
               
               
                 154 
                 GPELCSVQL 
                 0.018 
               
               
                 22 
                 QPISYELRV 
                 0.018 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XIII 
               
             
            
               
                   
               
               
                 158P3D2v.1-A3-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 178 
                 RLRGwWPVVK 
                 90.000 
               
               
                 281 
                 KTFVfFIWRR 
                 40.500 
               
               
                 187 
                 KLKEaEDVER 
                 18.000 
               
               
                 241 
                 ELLTvEEAEK 
                 9.000 
               
               
                 299 
                 LLVLlTVFLL 
                 8.100 
               
               
                 302 
                 LLTVfLLLVF 
                 6.000 
               
               
                 122 
                 LVLQvWDYDR 
                 5.400 
               
               
                 120 
                 AVLVlQVWDY 
                 5.400 
               
               
                 297 
                 LLLLvLLTVF 
                 4.500 
               
               
                 231 
                 ILTGkVEAEF 
                 4.500 
               
               
                 242 
                 LLTVeEAEKR 
                 4.000 
               
               
                 301 
                 VLLTvFLLLV 
                 2.700 
               
               
                 144 
                 QLPDmVRGAR 
                 1.800 
               
               
                 319 
                 SQVIfRPLHK 
                 1.800 
               
               
                 296 
                 VLLLlVLLTV 
                 1.350 
               
               
                 294 
                 TLVLlLLVLL 
                 1.350 
               
               
                 10 
                 VPAPpPVDIK 
                 1.350 
               
               
                 48 
                 GEMSsDIYVK 
                 1.215 
               
               
                 161 
                 QLARnGAGPR 
                 1.200 
               
               
                 298 
                 LLLVlLTVFL 
                 0.900 
               
               
                 77 
                 LTGEgNFNWR 
                 0.900 
               
               
                 276 
                 FVNPlKTFVF 
                 0.900 
               
               
                 76 
                 SLTGeGNFNW 
                 0.900 
               
               
                 300 
                 LVLLtVFLLL 
                 0.810 
               
               
                 123 
                 VLQVwDYDRI 
                 0.600 
               
               
                 303 
                 LTVFlLLVFY 
                 0.450 
               
               
                 304 
                 TVFLlLVFYT 
                 0.450 
               
               
                 81 
                 GNFNwRFVFR 
                 0.360 
               
               
                 17 
                 DIKPrQPISY 
                 0.360 
               
               
                 166 
                 GAGPrCNLFR 
                 0.360 
               
               
                 31 
                 VIWNtEDVVL 
                 0.300 
               
               
                 107 
                 GPFAlEEAEF 
                 0.300 
               
               
                 46 
                 LTGEmSSDIY 
                 0.300 
               
               
                 198 
                 AQEAqAGKKK 
                 0.300 
               
               
                 279 
                 PLKTfVFFIW 
                 0.270 
               
               
                 278 
                 NPLKtFVFFI 
                 0.243 
               
               
                 180 
                 RGWWpVVKLK 
                 0.225 
               
               
                 93 
                 YLPTeREVSV 
                 0.200 
               
               
                 140 
                 SLELqLPDMV 
                 0.200 
               
               
                 172 
                 NLFRcRRLRG 
                 0.200 
               
               
                 125 
                 QVWDyDRISA 
                 0.200 
               
               
                 307 
                 LLLVfYTIPG 
                 0.180 
               
               
                 235 
                 KVEAeFELLT 
                 0.180 
               
               
                 96 
                 TEREvSVWRR 
                 0.162 
               
               
                 226 
                 GGNVyILTGK 
                 0.135 
               
               
                 293 
                 RTLVlLLLVL 
                 0.135 
               
               
                 309 
                 LVFYtIPGQI 
                 0.135 
               
               
                 224 
                 DMGGnVYILT 
                 0.135 
               
               
                 271 
                 TSFNwFVNPL 
                 0.135 
               
               
                 313 
                 TIPGqISQVI 
                 0.135 
               
               
                 256 
                 GRKQpEPLEK 
                 0.120 
               
               
                 87 
                 FVFRfDYLPT 
                 0.100 
               
               
                 101 
                 SVWRrSGPFA 
                 0.100 
               
               
                 52 
                 SDIYvKSWVK 
                 0.090 
               
               
                 295 
                 LVLLlLVLLT 
                 0.090 
               
               
                 148 
                 MVRGaRGPEL 
                 0.090 
               
               
                 306 
                 FLLLvFYTIP 
                 0.090 
               
               
                 45 
                 PLTGeMSSDI 
                 0.090 
               
               
                 286 
                 FIWRrYWRTL 
                 0.090 
               
               
                 19 
                 KPRQpISYEL 
                 0.081 
               
               
                 280 
                 LKTFvFFIWR 
                 0.072 
               
               
                 62 
                 GLEHdKQETD 
                 0.060 
               
               
                 259 
                 QPEPlEKPSR 
                 0.060 
               
               
                 284 
                 VFFIwRRYWR 
                 0.060 
               
               
                 196 
                 REAQeAQAGK 
                 0.060 
               
               
                 82 
                 NFNWrFVFRF 
                 0.054 
               
               
                 141 
                 LELQlPDMVR 
                 0.054 
               
               
                 121 
                 VLVLqVWDYD 
                 0.045 
               
               
                 308 
                 LLVFyTIPGQ 
                 0.045 
               
               
                 12 
                 APPPvDIKPR 
                 0.045 
               
               
                 39 
                 VLDDeNPLTG 
                 0.040 
               
               
                 84 
                 NWRFvFRFDY 
                 0.036 
               
               
                 168 
                 GPRCnLFRCR 
                 0.036 
               
               
                 117 
                 RQPAvLVLQV 
                 0.036 
               
               
                 21 
                 RQPIsYELRV 
                 0.036 
               
               
                 312 
                 YTIPgQISQV 
                 0.034 
               
               
                 272 
                 SFNWfVNPLK 
                 0.030 
               
               
                 58 
                 SWVKgLEHDK 
                 0.030 
               
               
                 200 
                 EAQAgKKKRK 
                 0.030 
               
               
                 30 
                 VVIWnTEDVV 
                 0.030 
               
               
                 283 
                 FVFFiWRRYW 
                 0.030 
               
               
                 137 
                 FLGSlELQLP 
                 0.030 
               
               
                 222 
                 FTDMgGNVYI 
                 0.030 
               
               
                 29 
                 RVVIwNTEDV 
                 0.030 
               
               
                 95 
                 PTEReVSVWR 
                 0.030 
               
               
                 37 
                 DVVLdDENPL 
                 0.027 
               
               
                 78 
                 TGEGnFNWRF 
                 0.027 
               
               
                 9 
                 DVPApPPVDI 
                 0.027 
               
               
                 317 
                 QISQvIFRPL 
                 0.027 
               
               
                 270 
                 KTSFnWFVNP 
                 0.027 
               
               
                 246 
                 EEAEkRPVGK 
                 0.027 
               
               
                 197 
                 EAQEaQAGKK 
                 0.027 
               
               
                 131 
                 RISAnDFLGS 
                 0.024 
               
               
                 24 
                 ISYElRVVIW 
                 0.022 
               
               
                 261 
                 EPLEkPSRPK 
                 0.020 
               
               
                 314 
                 IPGQiSQVIF 
                 0.020 
               
               
                 202 
                 QAGKkKRKQR 
                 0.020 
               
               
                 89 
                 FRFDyLPTER 
                 0.020 
               
               
                 185 
                 VVKLkEAEDV 
                 0.020 
               
               
                 316 
                 GQISqVIFRP 
                 0.018 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XIV 
               
             
            
               
                   
               
               
                 158P3D2v.1-A1101-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 320 
                 QVIFRPLHK 
                 6.000 
               
               
                 281 
                 KTFVFFIWR 
                 2.400 
               
               
                 59 
                 WVKGLEHDK 
                 2.000 
               
               
                 316 
                 GQISQVIFR 
                 1.080 
               
               
                 198 
                 AQEAQAGKK 
                 0.600 
               
               
                 53 
                 DIYVKSWVK 
                 0.480 
               
               
                 242 
                 LLTVEEAEK 
                 0.400 
               
               
                 21 
                 RQPISYELR 
                 0.360 
               
               
                 243 
                 LTVEEAEKR 
                 0.300 
               
               
                 201 
                 AQAGKKKRK 
                 0.300 
               
               
                 49 
                 EMSSDIYVK 
                 0.240 
               
               
                 227 
                 GNVYILTGK 
                 0.180 
               
               
                 123 
                 VLQVWDYDR 
                 0.160 
               
               
                 257 
                 RKQPEPLEK 
                 0.120 
               
               
                 90 
                 RFDYLPTER 
                 0.120 
               
               
                 282 
                 TFVFFIWRR 
                 0.120 
               
               
                 170 
                 RCNLFRCRR 
                 0.120 
               
               
                 285 
                 FFIWRRYWR 
                 0.120 
               
               
                 293 
                 RTLVLLLLV 
                 0.090 
               
               
                 300 
                 LVLLTVFLL 
                 0.090 
               
               
                 273 
                 FNWFVNPLK 
                 0.080 
               
               
                 181 
                 GWWPVVKLK 
                 0.060 
               
               
                 250 
                 KRPVGKGRK 
                 0.060 
               
               
                 109 
                 FALEEAEFR 
                 0.060 
               
               
                 247 
                 EAEKRPVGK 
                 0.060 
               
               
                 197 
                 EAQEAQAGK 
                 0.060 
               
               
                 235 
                 KVEAEFELL 
                 0.060 
               
               
                 142 
                 ELQLPDMVR 
                 0.048 
               
               
                 156 
                 ELCSVQLAR 
                 0.048 
               
               
                 145 
                 LPDMVRGAR 
                 0.040 
               
               
                 304 
                 TVFLLLVFY 
                 0.040 
               
               
                 82 
                 NFNWRFVFR 
                 0.040 
               
               
                 101 
                 SVWRRSGPF 
                 0.040 
               
               
                 228 
                 NVYILTGKV 
                 0.040 
               
               
                 162 
                 LARNGAGPR 
                 0.040 
               
               
                 295 
                 LVLLLLVLL 
                 0.030 
               
               
                 77 
                 LTGEGNFNW 
                 0.030 
               
               
                 199 
                 QEAQAGKKK 
                 0.030 
               
               
                 303 
                 LTVFLLLVF 
                 0.030 
               
               
                 38 
                 VVLDDENPL 
                 0.030 
               
               
                 30 
                 VVIWNTEDV 
                 0.030 
               
               
                 290 
                 RYWRTLVLL 
                 0.024 
               
               
                 276 
                 FVNPLKTFV 
                 0.020 
               
               
                 179 
                 LRGWWPVVK 
                 0.020 
               
               
                 11 
                 PAPPPVDIK 
                 0.020 
               
               
                 159 
                 SVQLARNGA 
                 0.020 
               
               
                 172 
                 NLFRCRRLR 
                 0.016 
               
               
                 204 
                 GKKKRKQRR 
                 0.012 
               
               
                 306 
                 FLLLVFYTI 
                 0.012 
               
               
                 301 
                 VLLTVFLLL 
                 0.012 
               
               
                 121 
                 VLVLQVWDY 
                 0.012 
               
               
                 96 
                 TEREVSVWR 
                 0.012 
               
               
                 178 
                 RLRGWWPVV 
                 0.012 
               
               
                 297 
                 LLLLVLLTV 
                 0.012 
               
               
                 294 
                 TLVLLLLVL 
                 0.012 
               
               
                 232 
                 LTGKVEAEF 
                 0.010 
               
               
                 222 
                 FTDMGGNVY 
                 0.010 
               
               
                 55 
                 YVKSWVKGL 
                 0.010 
               
               
                 46 
                 LTGEMSSDI 
                 0.010 
               
               
                 29 
                 RVVIWNTED 
                 0.009 
               
               
                 124 
                 LQVWDYDRI 
                 0.009 
               
               
                 270 
                 KTSFNWFVN 
                 0.009 
               
               
                 86 
                 RFVFRFDYL 
                 0.009 
               
               
                 319 
                 SQVIFRPLH 
                 0.009 
               
               
                 302 
                 LLTVFLLLV 
                 0.008 
               
               
                 87 
                 FVFRFDYLP 
                 0.008 
               
               
                 137 
                 FLGSLELQL 
                 0.008 
               
               
                 167 
                 AGPRCNLFR 
                 0.008 
               
               
                 31 
                 VIWNTEDVV 
                 0.008 
               
               
                 81 
                 GNFNWRFVF 
                 0.007 
               
               
                 48 
                 GEMSSDIYV 
                 0.007 
               
               
                 208 
                 RKQRRRKGR 
                 0.006 
               
               
                 206 
                 KKRKQRRRK 
                 0.006 
               
               
                 154 
                 GPELCSVQL 
                 0.006 
               
               
                 230 
                 YILTGKVEA 
                 0.006 
               
               
                 22 
                 QPISYELRV 
                 0.006 
               
               
                 299 
                 LLVLLTVFL 
                 0.006 
               
               
                 193 
                 DVEREAQEA 
                 0.006 
               
               
                 298 
                 LLLVLLTVF 
                 0.006 
               
               
                 265 
                 KPSRPKTSF 
                 0.006 
               
               
                 166 
                 GAGPRCNLF 
                 0.006 
               
               
                 200 
                 EAQAGKKKR 
                 0.006 
               
               
                 175 
                 RCRRLRGWW 
                 0.006 
               
               
                 268 
                 RPKTSFNWF 
                 0.006 
               
               
                 262 
                 PLEKPSRPK 
                 0.004 
               
               
                 25 
                 SYELRVVIW 
                 0.004 
               
               
                 2 
                 WIDIFPQDV 
                 0.004 
               
               
                 78 
                 TGEGNFNWR 
                 0.004 
               
               
                 188 
                 LKEAEDVER 
                 0.004 
               
               
                 309 
                 LVFYTIPGQ 
                 0.004 
               
               
                 118 
                 QPAVLVLQV 
                 0.004 
               
               
                 313 
                 TIPGQISQV 
                 0.004 
               
               
                 283 
                 FVFFIWRRY 
                 0.004 
               
               
                 310 
                 VFYTIPGQI 
                 0.004 
               
               
                 140 
                 SLELQLPDM 
                 0.004 
               
               
                 131 
                 RISANDFLG 
                 0.004 
               
               
                 79 
                 GEGNFNWRF 
                 0.004 
               
               
                 312 
                 YTIPGQISQ 
                 0.003 
               
               
                 278 
                 NPLKTFVFF 
                 0.003 
               
               
                 120 
                 AVLVLQVWD 
                 0.003 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XV 
               
             
            
               
                   
               
               
                 158P3D2v.1-A1101-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 281 
                 KTFVfFIWRR 
                 2.400 
               
               
                 319 
                 SQVIfRPLHK 
                 1.800 
               
               
                 122 
                 LVLQvWDYDR 
                 1.200 
               
               
                 178 
                 RLRGwWPVVK 
                 1.200 
               
               
                 48 
                 GEMSsDIYVK 
                 0.720 
               
               
                 198 
                 AQEAqAGKKK 
                 0.300 
               
               
                 166 
                 GAGPrCNLFR 
                 0.240 
               
               
                 187 
                 KLKEaEDVER 
                 0.240 
               
               
                 272 
                 SFNWfVNPLK 
                 0.200 
               
               
                 10 
                 VPAPpPVDIK 
                 0.200 
               
               
                 77 
                 LTGEgNFNWR 
                 0.200 
               
               
                 241 
                 ELLTvEEAEK 
                 0.180 
               
               
                 196 
                 REAQeAQAGK 
                 0.180 
               
               
                 284 
                 VFFIwRRYWR 
                 0.160 
               
               
                 256 
                 GRKQpEPLEK 
                 0.120 
               
               
                 29 
                 RVVIwNTEDV 
                 0.090 
               
               
                 293 
                 RTLVlLLLVL 
                 0.090 
               
               
                 125 
                 QVWDyDRISA 
                 0.080 
               
               
                 144 
                 QLPDmVRGAR 
                 0.080 
               
               
                 161 
                 QLARnGAGPR 
                 0.080 
               
               
                 242 
                 LLTVeEAEKR 
                 0.080 
               
               
                 226 
                 GGNVyILTGK 
                 0.060 
               
               
                 180 
                 RGWWpVVKLK 
                 0.060 
               
               
                 52 
                 SDIYvKSWVK 
                 0.060 
               
               
                 120 
                 AVLVlQVWDY 
                 0.060 
               
               
                 300 
                 LVLLtVFLLL 
                 0.060 
               
               
                 197 
                 EAQEaQAGKK 
                 0.060 
               
               
                 276 
                 FVNPlKTFVF 
                 0.060 
               
               
                 81 
                 GNFNwRFVFR 
                 0.048 
               
               
                 290 
                 RYWRtLVLLL 
                 0.048 
               
               
                 101 
                 SVWRrSGPFA 
                 0.040 
               
               
                 259 
                 QPEPlEKPSR 
                 0.040 
               
               
                 309 
                 LVFYtIPGQI 
                 0.040 
               
               
                 21 
                 RQPIsYELRV 
                 0.036 
               
               
                 141 
                 LELQlPDMVR 
                 0.036 
               
               
                 117 
                 RQPAvLVLQV 
                 0.036 
               
               
                 58 
                 SWVKgLEHDK 
                 0.030 
               
               
                 200 
                 EAQAgKKKRK 
                 0.030 
               
               
                 30 
                 VVIWnTEDVV 
                 0.030 
               
               
                 96 
                 TEREvSVWRR 
                 0.024 
               
               
                 12 
                 APPPvDIKPR 
                 0.020 
               
               
                 148 
                 MVRGaRGPEL 
                 0.020 
               
               
                 202 
                 QAGKkKRKQR 
                 0.020 
               
               
                 185 
                 VVKLkEAEDV 
                 0.020 
               
               
                 95 
                 PTEReVSVWR 
                 0.020 
               
               
                 299 
                 LLVLlTVFLL 
                 0.018 
               
               
                 246 
                 EEAEkRPVGK 
                 0.018 
               
               
                 303 
                 LTVFlLLVFY 
                 0.015 
               
               
                 312 
                 YTIPgQISQV 
                 0.015 
               
               
                 168 
                 GPRCnLFRCR 
                 0.012 
               
               
                 235 
                 KVEAeFELLT 
                 0.012 
               
               
                 19 
                 KPRQpISYEL 
                 0.012 
               
               
                 304 
                 TVFLlLVFYT 
                 0.012 
               
               
                 296 
                 VLLLlVLLTV 
                 0.012 
               
               
                 107 
                 GPFAlEEAEF 
                 0.012 
               
               
                 76 
                 SLTGeGNFNW 
                 0.012 
               
               
                 301 
                 VLLTvFLLLV 
                 0.012 
               
               
                 268 
                 RPKTsFNWFV 
                 0.012 
               
               
                 222 
                 FTDMgGNVYI 
                 0.010 
               
               
                 46 
                 LTGEmSSDIY 
                 0.010 
               
               
                 37 
                 DVVLdDENPL 
                 0.009 
               
               
                 261 
                 EPLEkPSRPK 
                 0.009 
               
               
                 278 
                 NPLKtFVFFI 
                 0.009 
               
               
                 316 
                 GQISqVIFRP 
                 0.008 
               
               
                 280 
                 LKTFvFFIWR 
                 0.008 
               
               
                 87 
                 FVFRfDYLPT 
                 0.008 
               
               
                 302 
                 LLTVfLLLVF 
                 0.008 
               
               
                 89 
                 FRFDyLPTER 
                 0.008 
               
               
                 31 
                 VIWNtEDVVL 
                 0.008 
               
               
                 207 
                 KRKQrRRKGR 
                 0.006 
               
               
                 205 
                 KKKRkQRRRK 
                 0.006 
               
               
                 216 
                 RPEDlEFTDM 
                 0.006 
               
               
                 249 
                 EKRPvGKGRK 
                 0.006 
               
               
                 294 
                 TLVLlLLVLL 
                 0.006 
               
               
                 305 
                 VFLLlVFYTI 
                 0.006 
               
               
                 82 
                 NFNWrFVFRF 
                 0.006 
               
               
                 297 
                 LLLLvLLTVF 
                 0.006 
               
               
                 199 
                 QEAQaGKKKR 
                 0.006 
               
               
                 295 
                 LVLLlLVLLT 
                 0.006 
               
               
                 154 
                 GPELcSVQLA 
                 0.006 
               
               
                 151 
                 GARGpELCSV 
                 0.006 
               
               
                 9 
                 DVPApPPVDI 
                 0.006 
               
               
                 298 
                 LLLVlLTVFL 
                 0.006 
               
               
                 248 
                 AEKRpVGKGR 
                 0.006 
               
               
                 229 
                 VYILtGKVEA 
                 0.006 
               
               
                 67 
                 KQETdVHFNS 
                 0.005 
               
               
                 123 
                 VLQVwDYDRI 
                 0.004 
               
               
                 93 
                 YLPTeREVSV 
                 0.004 
               
               
                 283 
                 FVFFiWRRYW 
                 0.004 
               
               
                 203 
                 AGKKkRKQRR 
                 0.004 
               
               
                 231 
                 ILTGkVEAEF 
                 0.004 
               
               
                 108 
                 PFALeEAEFR 
                 0.004 
               
               
                 140 
                 SLELqLPDMV 
                 0.004 
               
               
                 313 
                 TIPGqISQVI 
                 0.004 
               
               
                 155 
                 PELCsVQLAR 
                 0.004 
               
               
                 38 
                 VVLDdENPLT 
                 0.003 
               
               
                 275 
                 WFVNpLKTFV 
                 0.003 
               
               
                 270 
                 KTSFnWFVNP 
                 0.003 
               
               
                 54 
                 IYVKsWVKGL 
                 0.003 
               
               
                 131 
                 RISAnDFLGS 
                 0.002 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XVI 
               
             
            
               
                   
               
               
                 158P3D2v.1-A24-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 290 
                 RYWRTLVLL 
                 400.000 
               
               
                 86 
                 RFVFRFDYL 
                 72.000 
               
               
                 272 
                 SFNWFVNPL 
                 36.000 
               
               
                 275 
                 WFVNPLKTF 
                 15.000 
               
               
                 235 
                 KVEAEFELL 
                 14.400 
               
               
                 318 
                 ISQVIFRPL 
                 10.080 
               
               
                 301 
                 VLLTVFLLL 
                 10.080 
               
               
                 92 
                 DYLPTEREV 
                 9.900 
               
               
                 180 
                 RGWWPVVKL 
                 8.800 
               
               
                 38 
                 VVLDDENPL 
                 8.640 
               
               
                 255 
                 KGRKQPEPL 
                 8.000 
               
               
                 25 
                 SYELRVVIW 
                 7.500 
               
               
                 299 
                 LLVLLTVFL 
                 7.200 
               
               
                 294 
                 TLVLLLLVL 
                 7.200 
               
               
                 295 
                 LVLLLLVLL 
                 7.200 
               
               
                 133 
                 SANDFLGSL 
                 7.200 
               
               
                 310 
                 VFYTIPGQI 
                 7.000 
               
               
                 171 
                 CNLFRCRRL 
                 6.000 
               
               
                 32 
                 IWNTEDVVL 
                 6.000 
               
               
                 154 
                 GPELCSVQL 
                 6.000 
               
               
                 311 
                 FYTIPGQIS 
                 6.000 
               
               
                 300 
                 LVLLTVFLL 
                 6.000 
               
               
                 268 
                 RPKTSFNWF 
                 5.760 
               
               
                 69 
                 ETDVHFNSL 
                 5.760 
               
               
                 214 
                 KGRPEDLEF 
                 5.280 
               
               
                 137 
                 FLGSLELQL 
                 4.800 
               
               
                 291 
                 YWRTLVLLL 
                 4.800 
               
               
                 55 
                 YVKSWVKGL 
                 4.000 
               
               
                 224 
                 DMGGNVYIL 
                 4.000 
               
               
                 165 
                 NGAGPRCNL 
                 4.000 
               
               
                 287 
                 IWRRYWRTL 
                 4.000 
               
               
                 265 
                 KPSRPKTSF 
                 4.000 
               
               
                 303 
                 LTVFLLLVF 
                 3.600 
               
               
                 298 
                 LLLVLLTVF 
                 3.600 
               
               
                 278 
                 NPLKTFVFF 
                 3.600 
               
               
                 232 
                 LTGKVEAEF 
                 3.080 
               
               
                 277 
                 VNPLKTFVF 
                 3.000 
               
               
                 75 
                 NSLTGEGNF 
                 3.000 
               
               
                 166 
                 GAGPRCNLF 
                 2.880 
               
               
                 306 
                 FLLLVFYTI 
                 2.520 
               
               
                 83 
                 FNWRFVFRF 
                 2.000 
               
               
                 101 
                 SVWRRSGPF 
                 2.000 
               
               
                 81 
                 GNFNWRFVF 
                 2.000 
               
               
                 314 
                 IPGQISQVI 
                 1.680 
               
               
                 124 
                 LQVWDYDRI 
                 1.500 
               
               
                 24 
                 ISYELRVVI 
                 1.440 
               
               
                 10 
                 VPAPPPVDI 
                 1.200 
               
               
                 46 
                 LTGEMSSDI 
                 1.200 
               
               
                 108 
                 PFALEEAEF 
                 1.100 
               
               
                 305 
                 VFLLLVFYT 
                 0.900 
               
               
                 54 
                 IYVKSWVKG 
                 0.825 
               
               
                 289 
                 RRYWRTLVL 
                 0.800 
               
               
                 212 
                 RRKGRPEDL 
                 0.800 
               
               
                 234 
                 GKVEAEFEL 
                 0.792 
               
               
                 229 
                 VYILTGKVE 
                 0.750 
               
               
                 140 
                 SLELQLPDM 
                 0.750 
               
               
                 116 
                 FRQPAVLVL 
                 0.720 
               
               
                 128 
                 DYDRISAND 
                 0.700 
               
               
                 221 
                 EFTDMGGNV 
                 0.600 
               
               
                 130 
                 DRISANDFL 
                 0.600 
               
               
                 292 
                 WRTLVLLLL 
                 0.560 
               
               
                 115 
                 EFRQPAVLV 
                 0.500 
               
               
                 88 
                 VFRFDYLPT 
                 0.500 
               
               
                 284 
                 VFFIWRRYW 
                 0.500 
               
               
                 149 
                 VRGARGPEL 
                 0.440 
               
               
                 135 
                 NDFLGSLEL 
                 0.440 
               
               
                 114 
                 AEFRQPAVL 
                 0.400 
               
               
                 103 
                 WRRSGPFAL 
                 0.400 
               
               
                 66 
                 DKQETDVHF 
                 0.360 
               
               
                 293 
                 RTLVLLLLV 
                 0.360 
               
               
                 315 
                 PGQISQVIF 
                 0.300 
               
               
                 67 
                 KQETDVHFN 
                 0.300 
               
               
                 190 
                 EAEDVEREA 
                 0.277 
               
               
                 129 
                 YDRISANDF 
                 0.240 
               
               
                 175 
                 RCRRLRGWW 
                 0.240 
               
               
                 259 
                 QPEPLEKPS 
                 0.216 
               
               
                 276 
                 FVNPLKTFV 
                 0.216 
               
               
                 297 
                 LLLLVLLTV 
                 0.210 
               
               
                 150 
                 RGARGPELC 
                 0.200 
               
               
                 270 
                 KTSFNWFVN 
                 0.200 
               
               
                 164 
                 RNGAGPRCN 
                 0.200 
               
               
                 79 
                 GEGNFNWRF 
                 0.200 
               
               
                 178 
                 RLRGWWPVV 
                 0.200 
               
               
                 193 
                 DVEREAQEA 
                 0.198 
               
               
                 62 
                 GLEHDKQET 
                 0.198 
               
               
                 50 
                 MSSDIYVKS 
                 0.185 
               
               
                 16 
                 VDIKPRQPI 
                 0.180 
               
               
                 296 
                 VLLLLVLLT 
                 0.180 
               
               
                 159 
                 SVQLARNGA 
                 0.180 
               
               
                 144 
                 QLPDMVRGA 
                 0.180 
               
               
                 106 
                 SGPFALEEA 
                 0.165 
               
               
                 183 
                 WPVVKLKEA 
                 0.165 
               
               
                 230 
                 YILTGKVEA 
                 0.165 
               
               
                 80 
                 EGNFNWRFV 
                 0.150 
               
               
                 237 
                 EAEFELLTV 
                 0.150 
               
               
                 43 
                 ENPLTGEMS 
                 0.150 
               
               
                 44 
                 NPLTGEMSS 
                 0.150 
               
               
                 30 
                 VVIWNTEDV 
                 0.150 
               
               
                 223 
                 TDMGGNVYI 
                 0.150 
               
               
                 22 
                 QPISYELRV 
                 0.150 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XVII 
               
             
            
               
                   
               
               
                 158P3D2v.1-A24-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 290 
                 RYWRtLVLLL 
                 480.000 
               
               
                 54 
                 IYVKsWVKGL 
                 300.000 
               
               
                 128 
                 DYDRiSANDF 
                 120.000 
               
               
                 136 
                 DFLGsLELQL 
                 36.000 
               
               
                 115 
                 EFRQpAVLVL 
                 20.000 
               
               
                 82 
                 NFNWrFVFRF 
                 15.000 
               
               
                 153 
                 RGPElCSVQL 
                 14.400 
               
               
                 293 
                 RTLVlLLLVL 
                 14.400 
               
               
                 305 
                 VFLLlVFYTI 
                 12.600 
               
               
                 19 
                 KPRQpISYEL 
                 12.320 
               
               
                 170 
                 RCNLfRCRRL 
                 12.000 
               
               
                 25 
                 SYELrVVIWN 
                 10.500 
               
               
                 300 
                 LVLLtVFLLL 
                 10.080 
               
               
                 92 
                 DYLPtEREVS 
                 9.000 
               
               
                 229 
                 VYILtGKVEA 
                 8.250 
               
               
                 164 
                 RNGAgPRCNL 
                 8.000 
               
               
                 37 
                 DVVLdDENPL 
                 7.200 
               
               
                 294 
                 TLVLlLLVLL 
                 7.200 
               
               
                 298 
                 LLLVlLTVFL 
                 7.200 
               
               
                 317 
                 QISQvIFRPL 
                 6.720 
               
               
                 299 
                 LLVLlTVFLL 
                 6.000 
               
               
                 113 
                 EAEFrQPAVL 
                 6.000 
               
               
                 291 
                 YWRTlVLLLL 
                 5.600 
               
               
                 271 
                 TSFNwFVNPL 
                 4.800 
               
               
                 134 
                 ANDFlGSLEL 
                 4.400 
               
               
                 233 
                 TGKVeAEFEL 
                 4.400 
               
               
                 148 
                 MVRGaRGPEL 
                 4.400 
               
               
                 31 
                 VIWNtEDVVL 
                 4.000 
               
               
                 286 
                 FIWRrYWRTL 
                 4.000 
               
               
                 132 
                 ISANdFLGSL 
                 4.000 
               
               
                 102 
                 VWRRsGPFAL 
                 4.000 
               
               
                 277 
                 VNPLkTFVFF 
                 3.600 
               
               
                 276 
                 FVNPlKTFVF 
                 3.600 
               
               
                 297 
                 LLLLvLLTVF 
                 3.600 
               
               
                 231 
                 ILTGkVEAEF 
                 3.080 
               
               
                 80 
                 EGNFnWRFVF 
                 3.000 
               
               
                 78 
                 TGEGnFNWRF 
                 3.000 
               
               
                 100 
                 VSVWrRSGPF 
                 3.000 
               
               
                 313 
                 TIPGqISQVI 
                 2.520 
               
               
                 302 
                 LLTVfLLLVF 
                 2.400 
               
               
                 165 
                 NGAGpRCNLF 
                 2.400 
               
               
                 107 
                 GPFAlEEAEF 
                 2.200 
               
               
                 216 
                 RPEDlEFTDM 
                 2.160 
               
               
                 314 
                 IPGQiSQVIF 
                 2.000 
               
               
                 74 
                 FNSLtGEGNF 
                 2.000 
               
               
                 274 
                 NWFVnPLKTF 
                 2.000 
               
               
                 9 
                 DVPApPPVDI 
                 1.500 
               
               
                 278 
                 NPLKtFVFFI 
                 1.500 
               
               
                 123 
                 VLQVwDYDRI 
                 1.500 
               
               
                 309 
                 LVFYtIPGQI 
                 1.400 
               
               
                 282 
                 TFVFfIWRRY 
                 1.050 
               
               
                 222 
                 FTDMgGNVYI 
                 1.000 
               
               
                 275 
                 WFVNpLKTFV 
                 0.900 
               
               
                 139 
                 GSLElQLPDM 
                 0.900 
               
               
                 234 
                 GKVEaEFELL 
                 0.864 
               
               
                 239 
                 EFELlTVEEA 
                 0.825 
               
               
                 211 
                 RRRKgRPEDL 
                 0.800 
               
               
                 289 
                 RRYWrTLVLL 
                 0.800 
               
               
                 285 
                 FFIWrRYWRT 
                 0.750 
               
               
                 73 
                 HFNSlTGEGN 
                 0.750 
               
               
                 221 
                 EFTDmGGNVY 
                 0.720 
               
               
                 68 
                 QETDvHFNSL 
                 0.691 
               
               
                 223 
                 TDMGgNVYIL 
                 0.600 
               
               
                 310 
                 VFYTiPGQIS 
                 0.600 
               
               
                 311 
                 FYTIpGQISQ 
                 0.500 
               
               
                 173 
                 LFRCrRLRGW 
                 0.500 
               
               
                 85 
                 WRFVfRFDYL 
                 0.480 
               
               
                 61 
                 KGLEhDKQET 
                 0.475 
               
               
                 213 
                 RKGRpEDLEF 
                 0.440 
               
               
                 179 
                 LRGWwPVVKL 
                 0.440 
               
               
                 267 
                 SRPKtSFNWF 
                 0.432 
               
               
                 258 
                 KQPEpLEKPS 
                 0.432 
               
               
                 67 
                 KQETdVHFNS 
                 0.420 
               
               
                 129 
                 YDRIsANDFL 
                 0.400 
               
               
                 254 
                 GKGRkQPEPL 
                 0.400 
               
               
                 288 
                 WRRYwRTLVL 
                 0.400 
               
               
                 117 
                 RQPAvLVLQV 
                 0.360 
               
               
                 29 
                 RVVIwNTEDV 
                 0.300 
               
               
                 235 
                 KVEAeFELLT 
                 0.300 
               
               
                 264 
                 EKPSrPKTSF 
                 0.300 
               
               
                 21 
                 RQPIsYELRV 
                 0.300 
               
               
                 105 
                 RSGPfALEEA 
                 0.264 
               
               
                 214 
                 KGRPeDLEFT 
                 0.240 
               
               
                 131 
                 RISAnDFLGS 
                 0.240 
               
               
                 1 
                 MWIDiFPQDV 
                 0.216 
               
               
                 296 
                 VLLLlVLLTV 
                 0.210 
               
               
                 268 
                 RPKTsFNWFV 
                 0.200 
               
               
                 265 
                 KPSRpKTSFN 
                 0.200 
               
               
                 65 
                 HDKQeTDVHF 
                 0.200 
               
               
                 150 
                 RGARgPELCS 
                 0.200 
               
               
                 227 
                 GNVYiLTGKV 
                 0.198 
               
               
                 154 
                 GPELcSVQLA 
                 0.180 
               
               
                 303 
                 LTVFlLLVFY 
                 0.180 
               
               
                 38 
                 VVLDdENPLT 
                 0.180 
               
               
                 312 
                 YTIPgQISQV 
                 0.180 
               
               
                 158 
                 CSVQlARNGA 
                 0.180 
               
               
                 143 
                 LQLPdMVRGA 
                 0.180 
               
               
                 295 
                 LVLLlLVLLT 
                 0.180 
               
               
                 244 
                 TVEEaEKRPV 
                 0.180 
               
               
                 75 
                 NSLTgEGNFN 
                 0.180 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XVIII 
               
             
            
               
                   
               
               
                 158P3D2v.1-B7-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 255 
                 KGRKQPEPL 
                 40.000 
               
               
                 154 
                 GPELCSVQL 
                 24.000 
               
               
                 300 
                 LVLLTVFLL 
                 20.000 
               
               
                 55 
                 YVKSWVKGL 
                 20.000 
               
               
                 168 
                 GPRCNLFRC 
                 20.000 
               
               
                 295 
                 LVLLLLVLL 
                 20.000 
               
               
                 38 
                 VVLDDENPL 
                 20.000 
               
               
                 133 
                 SANDFLGSL 
                 12.000 
               
               
                 10 
                 VPAPPPVDI 
                 12.000 
               
               
                 165 
                 NGAGPRCNL 
                 9.000 
               
               
                 314 
                 IPGQISQVI 
                 8.000 
               
               
                 180 
                 RGWWPVVKL 
                 6.000 
               
               
                 235 
                 KVEAEFELL 
                 6.000 
               
               
                 294 
                 TLVLLLLVL 
                 4.000 
               
               
                 94 
                 LPTEREVSV 
                 4.000 
               
               
                 22 
                 QPISYELRV 
                 4.000 
               
               
                 301 
                 VLLTVFLLL 
                 4.000 
               
               
                 291 
                 YWRTLVLLL 
                 4.000 
               
               
                 318 
                 ISQVIFRPL 
                 4.000 
               
               
                 103 
                 WRRSGPFAL 
                 4.000 
               
               
                 299 
                 LLVLLTVFL 
                 4.000 
               
               
                 118 
                 QPAVLVLQV 
                 4.000 
               
               
                 137 
                 FLGSLELQL 
                 4.000 
               
               
                 287 
                 IWRRYWRTL 
                 4.000 
               
               
                 171 
                 CNLFRCRRL 
                 4.000 
               
               
                 224 
                 DMGGNVYIL 
                 4.000 
               
               
                 19 
                 KPRQPISYE 
                 3.000 
               
               
                 178 
                 RLRGWWPVV 
                 2.000 
               
               
                 183 
                 WPVVKLKEA 
                 2.000 
               
               
                 114 
                 AEFRQPAVL 
                 1.200 
               
               
                 69 
                 ETDVHFNSL 
                 1.200 
               
               
                 276 
                 FVNPLKTFV 
                 1.000 
               
               
                 27 
                 ELRVVIWNT 
                 1.000 
               
               
                 30 
                 VVIWNTEDV 
                 1.000 
               
               
                 228 
                 NVYILTGKV 
                 1.000 
               
               
                 151 
                 GARGPELCS 
                 0.900 
               
               
                 159 
                 SVQLARNGA 
                 0.750 
               
               
                 148 
                 MVRGARGPE 
                 0.750 
               
               
                 24 
                 ISYELRVVI 
                 0.600 
               
               
                 265 
                 KPSRPKTSF 
                 0.600 
               
               
                 12 
                 APPPVDIKP 
                 0.600 
               
               
                 292 
                 WRTLVLLLL 
                 0.400 
               
               
                 32 
                 IWNTEDVVL 
                 0.400 
               
               
                 289 
                 RRYWRTLVL 
                 0.400 
               
               
                 149 
                 VRGARGPEL 
                 0.400 
               
               
                 46 
                 LTGEMSSDI 
                 0.400 
               
               
                 306 
                 FLLLVFYTI 
                 0.400 
               
               
                 272 
                 SFNWFVNPL 
                 0.400 
               
               
                 234 
                 GKVEAEFEL 
                 0.400 
               
               
                 278 
                 NPLKTFVFF 
                 0.400 
               
               
                 130 
                 DRISANDFL 
                 0.400 
               
               
                 86 
                 RFVFRFDYL 
                 0.400 
               
               
                 135 
                 NDFLGSLEL 
                 0.400 
               
               
                 44 
                 NPLTGEMSS 
                 0.400 
               
               
                 212 
                 RRKGRPEDL 
                 0.400 
               
               
                 268 
                 RPKTSFNWF 
                 0.400 
               
               
                 290 
                 RYWRTLVLL 
                 0.400 
               
               
                 116 
                 FRQPAVLVL 
                 0.400 
               
               
                 124 
                 LQVWDYDRI 
                 0.400 
               
               
                 140 
                 SLELQLPDM 
                 0.300 
               
               
                 288 
                 WRRYWRTLV 
                 0.300 
               
               
                 162 
                 LARNGAGPR 
                 0.300 
               
               
                 115 
                 EFRQPAVLV 
                 0.300 
               
               
                 175 
                 RCRRLRGWW 
                 0.300 
               
               
                 214 
                 KGRPEDLEF 
                 0.200 
               
               
                 80 
                 EGNFNWRFV 
                 0.200 
               
               
                 302 
                 LLTVFLLLV 
                 0.200 
               
               
                 297 
                 LLLLVLLTV 
                 0.200 
               
               
                 261 
                 EPLEKPSRP 
                 0.200 
               
               
                 107 
                 GPFALEEAE 
                 0.200 
               
               
                 31 
                 VIWNTEDVV 
                 0.200 
               
               
                 313 
                 TIPGQISQV 
                 0.200 
               
               
                 251 
                 RPVGKGRKQ 
                 0.200 
               
               
                 293 
                 RTLVLLLLV 
                 0.200 
               
               
                 6 
                 FPQDVPAPP 
                 0.200 
               
               
                 237 
                 EAEFELLTV 
                 0.180 
               
               
                 113 
                 EAEFRQPAV 
                 0.180 
               
               
                 193 
                 DVEREAQEA 
                 0.150 
               
               
                 120 
                 AVLVLQVWD 
                 0.150 
               
               
                 259 
                 QPEPLEKPS 
                 0.120 
               
               
                 223 
                 TDMGGNVYI 
                 0.120 
               
               
                 283 
                 FVFFIWRRY 
                 0.100 
               
               
                 106 
                 SGPFALEEA 
                 0.100 
               
               
                 101 
                 SVWRRSGPF 
                 0.100 
               
               
                 150 
                 RGARGPELC 
                 0.100 
               
               
                 304 
                 TVFLLLVFY 
                 0.100 
               
               
                 42 
                 DENPLTGEM 
                 0.100 
               
               
                 296 
                 VLLLLVLLT 
                 0.100 
               
               
                 125 
                 QVWDYDRIS 
                 0.100 
               
               
                 225 
                 MGGNVYILT 
                 0.100 
               
               
                 144 
                 QLPDMVRGA 
                 0.100 
               
               
                 102 
                 VWRRSGPFA 
                 0.100 
               
               
                 4 
                 DIFPQDVPA 
                 0.100 
               
               
                 88 
                 VFRFDYLPT 
                 0.100 
               
               
                 209 
                 KQRRRKGRP 
                 0.100 
               
               
                 286 
                 FIWRRYWRT 
                 0.100 
               
               
                 230 
                 YILTGKVEA 
                 0.100 
               
               
                 16 
                 VDIKPRQPI 
                 0.090 
               
               
                 145 
                 LPDMVRGAR 
                 0.090 
               
               
                 190 
                 EAEDVEREA 
                 0.090 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XIX 
               
             
            
               
                   
               
               
                 158P3D2v.1-B7-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 19 
                 KPRQpISYEL 
                 800.000 
               
               
                 148 
                 MVRGaRGPEL 
                 200.000 
               
               
                 37 
                 DVVLdDENPL 
                 20.000 
               
               
                 300 
                 LVLLtVFLLL 
                 20.000 
               
               
                 164 
                 RNGAgPRCNL 
                 9.000 
               
               
                 278 
                 NPLKtFVFFI 
                 8.000 
               
               
                 151 
                 GARGpELCSV 
                 6.000 
               
               
                 216 
                 RPEDlEFTDM 
                 6.000 
               
               
                 31 
                 VIWNtEDVVL 
                 4.000 
               
               
                 298 
                 LLLVlLTVFL 
                 4.000 
               
               
                 294 
                 TLVLlLLVLL 
                 4.000 
               
               
                 129 
                 YDRIsANDFL 
                 4.000 
               
               
                 132 
                 ISANdFLGSL 
                 4.000 
               
               
                 288 
                 WRRYwRTLVL 
                 4.000 
               
               
                 170 
                 RCNLfRCRRL 
                 4.000 
               
               
                 22 
                 QPISyELRVV 
                 4.000 
               
               
                 115 
                 EFRQpAVLVL 
                 4.000 
               
               
                 153 
                 RGPElCSVQL 
                 4.000 
               
               
                 293 
                 RTLVlLLLVL 
                 4.000 
               
               
                 291 
                 YWRTlVLLLL 
                 4.000 
               
               
                 286 
                 FIWRrYWRTL 
                 4.000 
               
               
                 271 
                 TSFNwFVNPL 
                 4.000 
               
               
                 233 
                 TGKVeAEFEL 
                 4.000 
               
               
                 268 
                 RPKTsFNWFV 
                 4.000 
               
               
                 299 
                 LLVLlTVFLL 
                 4.000 
               
               
                 211 
                 RRRKgRPEDL 
                 4.000 
               
               
                 102 
                 VWRRsGPFAL 
                 4.000 
               
               
                 317 
                 QISQvIFRPL 
                 4.000 
               
               
                 134 
                 ANDFlGSLEL 
                 3.600 
               
               
                 113 
                 EAEFrQPAVL 
                 3.600 
               
               
                 9 
                 DVPApPPVDI 
                 3.000 
               
               
                 162 
                 LARNgAGPRC 
                 3.000 
               
               
                 309 
                 LVFYtIPGQI 
                 2.000 
               
               
                 168 
                 GPRCnLFRCR 
                 2.000 
               
               
                 223 
                 TDMGgNVYIL 
                 1.200 
               
               
                 30 
                 VVIWnTEDVV 
                 1.000 
               
               
                 29 
                 RVVIwNTEDV 
                 1.000 
               
               
                 214 
                 KGRPeDLEFT 
                 1.000 
               
               
                 185 
                 VVKLkEAEDV 
                 1.000 
               
               
                 139 
                 GSLElQLPDM 
                 1.000 
               
               
                 125 
                 QVWDyDRISA 
                 0.750 
               
               
                 12 
                 APPPvDIKPR 
                 0.600 
               
               
                 154 
                 GPELcSVQLA 
                 0.600 
               
               
                 179 
                 LRGWwPVVKL 
                 0.600 
               
               
                 295 
                 LVLLlLVLLT 
                 0.500 
               
               
                 38 
                 VVLDdENPLT 
                 0.500 
               
               
                 87 
                 FVFRfDYLPT 
                 0.500 
               
               
                 101 
                 SVWRrSGPFA 
                 0.500 
               
               
                 304 
                 TVFLlLVFYT 
                 0.500 
               
               
                 54 
                 IYVKsWVKGL 
                 0.400 
               
               
                 313 
                 TIPGqISQVI 
                 0.400 
               
               
                 289 
                 RRYWrTLVLL 
                 0.400 
               
               
                 136 
                 DFLGsLELQL 
                 0.400 
               
               
                 234 
                 GKVEaEFELL 
                 0.400 
               
               
                 254 
                 GKGRkQPEPL 
                 0.400 
               
               
                 118 
                 QPAVlVLQVW 
                 0.400 
               
               
                 314 
                 IPGQiSQVIF 
                 0.400 
               
               
                 68 
                 QETDvHFNSL 
                 0.400 
               
               
                 107 
                 GPFAlEEAEF 
                 0.400 
               
               
                 123 
                 VLQVwDYDRI 
                 0.400 
               
               
                 290 
                 RYWRtLVLLL 
                 0.400 
               
               
                 94 
                 LPTErEVSVW 
                 0.400 
               
               
                 265 
                 KPSRpKTSFN 
                 0.400 
               
               
                 85 
                 WRFVfRFDYL 
                 0.400 
               
               
                 261 
                 EPLEkPSRPK 
                 0.300 
               
               
                 10 
                 VPAPpPVDIK 
                 0.300 
               
               
                 120 
                 AVLVlQVWDY 
                 0.300 
               
               
                 167 
                 AGPRcNLFRC 
                 0.300 
               
               
                 287 
                 IWRRyWRTLV 
                 0.300 
               
               
                 244 
                 TVEEaEKRPV 
                 0.300 
               
               
                 251 
                 RPVGkGRKQP 
                 0.300 
               
               
                 6 
                 FPQDvPAPPP 
                 0.300 
               
               
                 296 
                 VLLLlVLLTV 
                 0.200 
               
               
                 117 
                 RQPAvLVLQV 
                 0.200 
               
               
                 44 
                 NPLTgEMSSD 
                 0.200 
               
               
                 176 
                 CRRLrGWWPV 
                 0.200 
               
               
                 183 
                 WPVVkLKEAE 
                 0.200 
               
               
                 301 
                 VLLTvFLLLV 
                 0.200 
               
               
                 227 
                 GNVYiLTGKV 
                 0.200 
               
               
                 21 
                 RQPIsYELRV 
                 0.200 
               
               
                 312 
                 YTIPgQISQV 
                 0.200 
               
               
                 93 
                 YLPTeREVSV 
                 0.200 
               
               
                 235 
                 KVEAeFELLT 
                 0.150 
               
               
                 158 
                 CSVQlARNGA 
                 0.150 
               
               
                 283 
                 FVFFiWRRYW 
                 0.150 
               
               
                 255 
                 KGRKqPEPLE 
                 0.150 
               
               
                 15 
                 PVDIkPRQPI 
                 0.135 
               
               
                 222 
                 FTDMgGNVYI 
                 0.120 
               
               
                 209 
                 KQRRrKGRPE 
                 0.100 
               
               
                 105 
                 RSGPfALEEA 
                 0.100 
               
               
                 27 
                 ELRVvIWNTE 
                 0.100 
               
               
                 273 
                 FNWFvNPLKT 
                 0.100 
               
               
                 143 
                 LQLPdMVRGA 
                 0.100 
               
               
                 175 
                 RCRRlRGWWP 
                 0.100 
               
               
                 276 
                 FVNPlKTFVF 
                 0.100 
               
               
                 61 
                 KGLEhDKQET 
                 0.100 
               
               
                 224 
                 DMGGnVYILT 
                 0.100 
               
               
                 178 
                 RLRGwWPVVK 
                 0.100 
               
               
                 194 
                 VEREaQEAQA 
                 0.100 
               
               
                 114 
                 AEFRqPAVLV 
                 0.090 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XX 
               
             
            
               
                   
               
               
                 158P3D2v.1-B3501-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 268 
                 RPKTSFNWF 
                 120.000 
               
               
                 265 
                 KPSRPKTSF 
                 40.000 
               
               
                 278 
                 NPLKTFVFF 
                 20.000 
               
               
                 214 
                 KGRPEDLEF 
                 9.000 
               
               
                 314 
                 IPGQISQVI 
                 8.000 
               
               
                 94 
                 LPTEREVSV 
                 8.000 
               
               
                 10 
                 VPAPPPVDI 
                 8.000 
               
               
                 154 
                 GPELCSVQL 
                 6.000 
               
               
                 168 
                 GPRCNLFRC 
                 6.000 
               
               
                 255 
                 KGRKQPEPL 
                 6.000 
               
               
                 133 
                 SANDFLGSL 
                 6.000 
               
               
                 318 
                 ISQVIFRPL 
                 5.000 
               
               
                 75 
                 NSLTGEGNF 
                 5.000 
               
               
                 118 
                 QPAVLVLQV 
                 4.000 
               
               
                 24 
                 ISYELRVVI 
                 4.000 
               
               
                 22 
                 QPISYELRV 
                 4.000 
               
               
                 38 
                 VVLDDENPL 
                 3.000 
               
               
                 55 
                 YVKSWVKGL 
                 3.000 
               
               
                 175 
                 RCRRLRGWW 
                 3.000 
               
               
                 166 
                 GAGPRCNLF 
                 3.000 
               
               
                 180 
                 RGWWPVVKL 
                 2.000 
               
               
                 183 
                 WPVVKLKEA 
                 2.000 
               
               
                 283 
                 FVFFIWRRY 
                 2.000 
               
               
                 304 
                 TVFLLLVFY 
                 2.000 
               
               
                 121 
                 VLVLQVWDY 
                 2.000 
               
               
                 44 
                 NPLTGEMSS 
                 2.000 
               
               
                 19 
                 KPRQPISYE 
                 1.200 
               
               
                 178 
                 RLRGWWPVV 
                 1.200 
               
               
                 299 
                 LLVLLTVFL 
                 1.000 
               
               
                 165 
                 NGAGPRCNL 
                 1.000 
               
               
                 224 
                 DMGGNVYIL 
                 1.000 
               
               
                 277 
                 VNPLKTFVF 
                 1.000 
               
               
                 298 
                 LLLVLLTVF 
                 1.000 
               
               
                 294 
                 TLVLLLLVL 
                 1.000 
               
               
                 137 
                 FLGSLELQL 
                 1.000 
               
               
                 171 
                 CNLFRCRRL 
                 1.000 
               
               
                 101 
                 SVWRRSGPF 
                 1.000 
               
               
                 81 
                 GNFNWRFVF 
                 1.000 
               
               
                 300 
                 LVLLTVFLL 
                 1.000 
               
               
                 50 
                 MSSDIYVKS 
                 1.000 
               
               
                 83 
                 FNWRFVFRF 
                 1.000 
               
               
                 232 
                 LTGKVEAEF 
                 1.000 
               
               
                 303 
                 LTVFLLLVF 
                 1.000 
               
               
                 301 
                 VLLTVFLLL 
                 1.000 
               
               
                 295 
                 LVLLLLVLL 
                 1.000 
               
               
                 77 
                 LTGEGNFNW 
                 1.000 
               
               
                 235 
                 KVEAEFELL 
                 0.900 
               
               
                 151 
                 GARGPELCS 
                 0.900 
               
               
                 46 
                 LTGEMSSDI 
                 0.800 
               
               
                 51 
                 SSDIYVKSW 
                 0.750 
               
               
                 132 
                 ISANDFLGS 
                 0.750 
               
               
                 222 
                 FTDMGGNVY 
                 0.600 
               
               
                 47 
                 TGEMSSDIY 
                 0.600 
               
               
                 259 
                 QPEPLEKPS 
                 0.600 
               
               
                 140 
                 SLELQLPDM 
                 0.600 
               
               
                 212 
                 RRKGRPEDL 
                 0.600 
               
               
                 124 
                 LQVWDYDRI 
                 0.600 
               
               
                 293 
                 RTLVLLLLV 
                 0.400 
               
               
                 306 
                 FLLLVFYTI 
                 0.400 
               
               
                 251 
                 RPVGKGRKQ 
                 0.400 
               
               
                 6 
                 FPQDVPAPP 
                 0.400 
               
               
                 261 
                 EPLEKPSRP 
                 0.400 
               
               
                 129 
                 YDRISANDF 
                 0.300 
               
               
                 291 
                 YWRTLVLLL 
                 0.300 
               
               
                 17 
                 DIKPRQPIS 
                 0.300 
               
               
                 27 
                 ELRVVIWNT 
                 0.300 
               
               
                 287 
                 IWRRYWRTL 
                 0.300 
               
               
                 69 
                 ETDVHFNSL 
                 0.300 
               
               
                 103 
                 WRRSGPFAL 
                 0.300 
               
               
                 237 
                 EAEFELLTV 
                 0.270 
               
               
                 216 
                 RPEDLEFTD 
                 0.240 
               
               
                 164 
                 RNGAGPRCN 
                 0.200 
               
               
                 234 
                 GKVEAEFEL 
                 0.200 
               
               
                 30 
                 VVIWNTEDV 
                 0.200 
               
               
                 313 
                 TIPGQISQV 
                 0.200 
               
               
                 18 
                 IKPRQPISY 
                 0.200 
               
               
                 150 
                 RGARGPELC 
                 0.200 
               
               
                 297 
                 LLLLVLLTV 
                 0.200 
               
               
                 42 
                 DENPLTGEM 
                 0.200 
               
               
                 107 
                 GPFALEEAE 
                 0.200 
               
               
                 290 
                 RYWRTLVLL 
                 0.200 
               
               
                 302 
                 LLTVFLLLV 
                 0.200 
               
               
                 12 
                 APPPVDIKP 
                 0.200 
               
               
                 31 
                 VIWNTEDVV 
                 0.200 
               
               
                 276 
                 FVNPLKTFV 
                 0.200 
               
               
                 228 
                 NVYILTGKV 
                 0.200 
               
               
                 125 
                 QVWDYDRIS 
                 0.200 
               
               
                 86 
                 RFVFRFDYL 
                 0.200 
               
               
                 144 
                 QLPDMVRGA 
                 0.200 
               
               
                 66 
                 DKQETDVHF 
                 0.200 
               
               
                 80 
                 EGNFNWRFV 
                 0.200 
               
               
                 85 
                 WRFVFRFDY 
                 0.200 
               
               
                 289 
                 RRYWRTLVL 
                 0.200 
               
               
                 270 
                 KTSFNWFVN 
                 0.200 
               
               
                 113 
                 EAEFRQPAV 
                 0.180 
               
               
                 190 
                 EAEDVEREA 
                 0.180 
               
               
                 76 
                 SLTGEGNFN 
                 0.150 
               
               
                 266 
                 PSRPKTSFN 
                 0.150 
               
               
                 32 
                 IWNTEDVVL 
                 0.150 
               
               
                 119 
                 PAVLVLQVW 
                 0.150 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXI 
               
             
            
               
                   
               
               
                 158P3D2v.1-B3501-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 19 
                 KPRQpISYEL 
                 120.000 
               
               
                 216 
                 RPEDlEFTDM 
                 72.000 
               
               
                 94 
                 LPTErEVSVW 
                 30.000 
               
               
                 107 
                 GPFAlEEAEF 
                 30.000 
               
               
                 268 
                 RPKTsFNWFV 
                 24.000 
               
               
                 139 
                 GSLElQLPDM 
                 20.000 
               
               
                 314 
                 IPGQiSQVIF 
                 20.000 
               
               
                 118 
                 QPAVlVLQVW 
                 10.000 
               
               
                 278 
                 NPLKtFVFFI 
                 8.000 
               
               
                 17 
                 DIKPrQPISY 
                 6.000 
               
               
                 22 
                 QPISyELRVV 
                 6.000 
               
               
                 271 
                 TSFNwFVNPL 
                 5.000 
               
               
                 24 
                 ISYElRVVIW 
                 5.000 
               
               
                 50 
                 MSSDiYVKSW 
                 5.000 
               
               
                 132 
                 ISANdFLGSL 
                 5.000 
               
               
                 100 
                 VSVWrRSGPF 
                 5.000 
               
               
                 46 
                 LTGEmSSDIY 
                 4.000 
               
               
                 153 
                 RGPElCSVQL 
                 4.000 
               
               
                 265 
                 KPSRpKTSFN 
                 4.000 
               
               
                 148 
                 MVRGaRGPEL 
                 3.000 
               
               
                 233 
                 TGKVeAEFEL 
                 3.000 
               
               
                 151 
                 GARGpELCSV 
                 2.700 
               
               
                 164 
                 RNGAgPRCNL 
                 2.000 
               
               
                 120 
                 AVLVlQVWDY 
                 2.000 
               
               
                 293 
                 RTLVlLLLVL 
                 2.000 
               
               
                 303 
                 LTVFlLLVFY 
                 2.000 
               
               
                 170 
                 RCNLfRCRRL 
                 2.000 
               
               
                 37 
                 DVVLdDENPL 
                 1.500 
               
               
                 31 
                 VIWNtEDVVL 
                 1.500 
               
               
                 298 
                 LLLVlLTVFL 
                 1.000 
               
               
                 317 
                 QISQvIFRPL 
                 1.000 
               
               
                 294 
                 TLVLlLLVLL 
                 1.000 
               
               
                 286 
                 FIWRrYWRTL 
                 1.000 
               
               
                 299 
                 LLVLlTVFLL 
                 1.000 
               
               
                 300 
                 LVLLtVFLLL 
                 1.000 
               
               
                 277 
                 VNPLkTFVFF 
                 1.000 
               
               
                 105 
                 RSGPfALEEA 
                 1.000 
               
               
                 302 
                 LLTVfLLLVF 
                 1.000 
               
               
                 74 
                 FNSLtGEGNF 
                 1.000 
               
               
                 231 
                 ILTGkVEAEF 
                 1.000 
               
               
                 80 
                 EGNFnWRFVF 
                 1.000 
               
               
                 297 
                 LLLLvLLTVF 
                 1.000 
               
               
                 165 
                 NGAGpRCNLF 
                 1.000 
               
               
                 276 
                 FVNPlKTFVF 
                 1.000 
               
               
                 113 
                 EAEFrQPAVL 
                 0.900 
               
               
                 185 
                 VVKLkEAEDV 
                 0.900 
               
               
                 214 
                 KGRPeDLEFT 
                 0.900 
               
               
                 162 
                 LARNgAGPRC 
                 0.900 
               
               
                 75 
                 NSLTgEGNFN 
                 0.750 
               
               
                 266 
                 PSRPkTSFNW 
                 0.750 
               
               
                 123 
                 VLQVwDYDRI 
                 0.600 
               
               
                 154 
                 GPELcSVQLA 
                 0.600 
               
               
                 84 
                 NWRFvFRFDY 
                 0.600 
               
               
                 211 
                 RRRKgRPEDL 
                 0.600 
               
               
                 61 
                 KGLEhDKQET 
                 0.600 
               
               
                 168 
                 GPRCnLFRCR 
                 0.600 
               
               
                 158 
                 CSVQlARNGA 
                 0.500 
               
               
                 283 
                 FVFFiWRRYW 
                 0.500 
               
               
                 76 
                 SLTGeGNFNW 
                 0.500 
               
               
                 9 
                 DVPApPPVDI 
                 0.400 
               
               
                 261 
                 EPLEkPSRPK 
                 0.400 
               
               
                 29 
                 RVVIwNTEDV 
                 0.400 
               
               
                 21 
                 RQPIsYELRV 
                 0.400 
               
               
                 251 
                 RPVGkGRKQP 
                 0.400 
               
               
                 309 
                 LVFYtIPGQI 
                 0.400 
               
               
                 6 
                 FPQDvPAPPP 
                 0.400 
               
               
                 258 
                 KQPEpLEKPS 
                 0.400 
               
               
                 117 
                 RQPAvLVLQV 
                 0.400 
               
               
                 313 
                 TIPGqISQVI 
                 0.400 
               
               
                 221 
                 EFTDmGGNVY 
                 0.400 
               
               
                 213 
                 RKGRpEDLEF 
                 0.300 
               
               
                 125 
                 QVWDyDRISA 
                 0.300 
               
               
                 129 
                 YDRIsANDFL 
                 0.300 
               
               
                 102 
                 VWRRsGPFAL 
                 0.300 
               
               
                 115 
                 EFRQpAVLVL 
                 0.300 
               
               
                 288 
                 WRRYwRTLVL 
                 0.300 
               
               
                 134 
                 ANDFlGSLEL 
                 0.300 
               
               
                 78 
                 TGEGnFNWRF 
                 0.300 
               
               
                 38 
                 VVLDdENPLT 
                 0.300 
               
               
                 234 
                 GKVEaEFELL 
                 0.300 
               
               
                 65 
                 HDKQeTDVHF 
                 0.300 
               
               
                 291 
                 YWRTlVLLLL 
                 0.300 
               
               
                 12 
                 APPPvDIKPR 
                 0.300 
               
               
                 131 
                 RISAnDFLGS 
                 0.300 
               
               
                 44 
                 NPLTgEMSSD 
                 0.300 
               
               
                 51 
                 SSDIyVKSWV 
                 0.300 
               
               
                 290 
                 RYWRtLVLLL 
                 0.200 
               
               
                 282 
                 TFVFfIWRRY 
                 0.200 
               
               
                 183 
                 WPVVkLKEAE 
                 0.200 
               
               
                 10 
                 VPAPpPVDIK 
                 0.200 
               
               
                 68 
                 QETDvHFNSL 
                 0.200 
               
               
                 227 
                 GNVYiLTGKV 
                 0.200 
               
               
                 93 
                 YLPTeREVSV 
                 0.200 
               
               
                 30 
                 VVIWnTEDVV 
                 0.200 
               
               
                 296 
                 VLLLlVLLTV 
                 0.200 
               
               
                 150 
                 RGARgPELCS 
                 0.200 
               
               
                 301 
                 VLLTvFLLLV 
                 0.200 
               
               
                 289 
                 RRYWrTLVLL 
                 0.200 
               
               
                 312 
                 YTIPgQISQV 
                 0.200 
               
               
                 187 
                 KLKEaEDVER 
                 0.180 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE VIII 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A1-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; 
               
               
                 each start position is Specified, the length 
               
               
                 of the peptide is 9 amino acids and the end 
               
               
                 position for each peptide is the start position 
               
               
                 plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1930 
                 FTDMGGNVY 
                 62.500 
               
               
                 626 
                 GAEGPEIPR 
                 45.000 
               
               
                 1553 
                 ETELTVAVF 
                 45.000 
               
               
                 1576 
                 HIDLENRFY 
                 25.000 
               
               
                 40 
                 EADIGELFR 
                 25.000 
               
               
                 217 
                 HGDPFQVSR 
                 25.000 
               
               
                 1349 
                 ELDWGSKYY 
                 25.000 
               
               
                 151 
                 ELEPGEAQL 
                 18.000 
               
               
                 427 
                 RAEGLPALR 
                 18.000 
               
               
                 197 
                 DVELSGVMF 
                 18.000 
               
               
                 583 
                 RAEPEPPQA 
                 18.000 
               
               
                 1474 
                 FSEPQISRG 
                 13.500 
               
               
                 1202 
                 ESEVLASYR 
                 13.500 
               
               
                 272 
                 YNEYFLFEF 
                 11.250 
               
               
                 1755 
                 TGEMSSDIY 
                 11.250 
               
               
                 1306 
                 SLDPFLAEA 
                 10.000 
               
               
                 668 
                 MIDPTVASQ 
                 10.000 
               
               
                 1598 
                 EVDGYNAWR 
                 10.000 
               
               
                 1511 
                 KADPYVVVS 
                 10.000 
               
               
                 452 
                 LVEPYVRVS 
                 9.000 
               
               
                 1146 
                 LVEPHSGRL 
                 9.000 
               
               
                 1121 
                 LIELDYSGR 
                 9.000 
               
               
                 267 
                 TSCPFYNEY 
                 7.500 
               
               
                 1011 
                 LSDPFARVL 
                 7.500 
               
               
                 786 
                 LQEVERLQR 
                 6.750 
               
               
                 1344 
                 IPDPEELDW 
                 6.250 
               
               
                 346 
                 ILDQPDGQF 
                 5.000 
               
               
                 1267 
                 HIVPHMLRF 
                 5.000 
               
               
                 327 
                 QTLPFMATR 
                 5.000 
               
               
                 1927 
                 DLEFTDMGG 
                 4.500 
               
               
                 703 
                 GTEGAAVEA 
                 4.500 
               
               
                 821 
                 GAERRTMTR 
                 4.500 
               
               
                 1466 
                 YPESEAVLF 
                 4.500 
               
               
                 1945 
                 EAEFELLTV 
                 4.500 
               
               
                 1200 
                 GVESEVLAS 
                 4.500 
               
               
                 1688 
                 HVETRPLYH 
                 4.500 
               
               
                 708 
                 AVEAQPLLG 
                 4.500 
               
               
                 1137 
                 EVEPQDLAP 
                 4.500 
               
               
                 585 
                 EPEPPQAQQ 
                 4.500 
               
               
                 235 
                 VLEAQKLVG 
                 4.500 
               
               
                 1818 
                 ALEEAEFRQ 
                 4.500 
               
               
                 1379 
                 DSDGVNLIS 
                 3.750 
               
               
                 1955 
                 EAEKRPVGK 
                 3.600 
               
               
                 1906 
                 AQEAQAGKK 
                 2.700 
               
               
                 316 
                 LTPPSPKAF 
                 2.500 
               
               
                 1659 
                 SGDPEEAQA 
                 2.500 
               
               
                 349 
                 QPDGQFYQR 
                 2.500 
               
               
                 1215 
                 FTELVRHLT 
                 2.250 
               
               
                 1967 
                 QPEPLEKPS 
                 2.250 
               
               
                 1786 
                 TGEGNFNWR 
                 2.250 
               
               
                 734 
                 RPEPMDGSG 
                 2.250 
               
               
                 745 
                 FCLPLCHCK 
                 2.000 
               
               
                 485 
                 FVELFPPLT 
                 1.800 
               
               
                 807 
                 ALEVLVAGS 
                 1.800 
               
               
                 1186 
                 EVEQPQVVL 
                 1.800 
               
               
                 1578 
                 DLENRFYSH 
                 1.800 
               
               
                 1821 
                 EAEFRQPAV 
                 1.800 
               
               
                 471 
                 SAEAAAPEW 
                 1.800 
               
               
                 1129 
                 RLEPSVPSE 
                 1.800 
               
               
                 881 
                 LAEEPQPPL 
                 1.800 
               
               
                 1184 
                 LLEVEQPQV 
                 1.800 
               
               
                 1848 
                 SLELQLPDM 
                 1.800 
               
               
                 543 
                 GSPPGAGLR 
                 1.500 
               
               
                 964 
                 TSELPPDLL 
                 1.350 
               
               
                 827 
                 MTRPNALDR 
                 1.250 
               
               
                 1989 
                 KTFVFFIWR 
                 1.250 
               
               
                 977 
                 AGLPSSLHR 
                 1.250 
               
               
                 1777 
                 ETDVHFNSL 
                 1.250 
               
               
                 1853 
                 LPDMVRGAR 
                 1.250 
               
               
                 512 
                 ATHVPDLRR 
                 1.250 
               
               
                 2020 
                 YTIPGQISQ 
                 1.250 
               
               
                 1073 
                 FGPPVFLGR 
                 1.250 
               
               
                 2011 
                 LTVFLLLVF 
                 1.250 
               
               
                 1097 
                 RPELQFFPL 
                 1.125 
               
               
                 1540 
                 FGEILELSI 
                 1.125 
               
               
                 1742 
                 NTEDVVLDD 
                 1.125 
               
               
                 1874 
                 GAGPRCNLF 
                 1.000 
               
               
                 368 
                 RAGTKGFIK 
                 1.000 
               
               
                 1159 
                 NVCPVLREF 
                 1.000 
               
               
                 2012 
                 TVFLLLVFY 
                 1.000 
               
               
                 2028 
                 QVIFRPLHK 
                 1.000 
               
               
                 1099 
                 ELQFFPLRK 
                 1.000 
               
               
                 506 
                 LVDAALATH 
                 1.000 
               
               
                 1621 
                 RCGLPAPEY 
                 1.000 
               
               
                 710 
                 EAQPLLGAR 
                 1.000 
               
               
                 1747 
                 VLDDENPLT 
                 1.000 
               
               
                 1029 
                 VLEQTLSPL 
                 0.900 
               
               
                 1057 
                 QEEPPLVII 
                 0.900 
               
               
                 1896 
                 LKEAEDVER 
                 0.900 
               
               
                 110 
                 QVELDLKYQ 
                 0.900 
               
               
                 291 
                 LLEITVSGV 
                 0.900 
               
               
                 947 
                 KLELFLRLG 
                 0.900 
               
               
                 689 
                 RLEEQLGRG 
                 0.900 
               
               
                 1397 
                 EAEVKGTVS 
                 0.900 
               
               
                 1193 
                 VLEVAGQGV 
                 0.900 
               
               
                 1943 
                 KVEAEFELL 
                 0.900 
               
               
                 1311 
                 LAEAGISRQ 
                 0.900 
               
               
                 1898 
                 EAEDVEREA 
                 0.900 
               
               
                 1770 
                 GLEHDKQET 
                 0.900 
               
               
                 1168 
                 RVEVLFWGL 
                 0.900 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IX 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A1-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; 
               
               
                 each start position is Specified, the length 
               
               
                 of the peptide is 10 amino acids and the end 
               
               
                 position for each peptide is the start position 
               
               
                 plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 452 
                 LVEPyVRVSF 
                 180.000 
               
               
                 1200 
                 GVESeVLASY 
                 90.000 
               
               
                 485 
                 FVELfPPLTR 
                 45.000 
               
               
                 1967 
                 QPEPlEKPSR 
                 45.000 
               
               
                 346 
                 ILDQpDGQFY 
                 25.000 
               
               
                 43 
                 IGELfRWPHY 
                 22.500 
               
               
                 1137 
                 EVEPqDLAPL 
                 18.000 
               
               
                 1011 
                 LSDPfARVLI 
                 15.000 
               
               
                 1474 
                 FSEPqISRGI 
                 13.500 
               
               
                 972 
                 LPEPsAGLPS 
                 11.250 
               
               
                 1215 
                 FTELvRHLTV 
                 11.250 
               
               
                 476 
                 APEWnEQLSF 
                 11.250 
               
               
                 1511 
                 KADPyVVVSA 
                 10.000 
               
               
                 1626 
                 APEYrAGAVK 
                 9.000 
               
               
                 807 
                 ALEVlVAGSR 
                 9.000 
               
               
                 1168 
                 RVEVlFWGLR 
                 9.000 
               
               
                 583 
                 RAEPePPQAQ 
                 9.000 
               
               
                 1129 
                 RLEPsVPSEV 
                 9.000 
               
               
                 1146 
                 LVEPhSGRLS 
                 9.000 
               
               
                 1306 
                 SLDPfLAEAG 
                 5.000 
               
               
                 1874 
                 GAGPrCNLFR 
                 5.000 
               
               
                 1984 
                 FVNPlKTFVF 
                 5.000 
               
               
                 1346 
                 DPEElDWGSK 
                 4.500 
               
               
                 401 
                 DIEKnLLLPR 
                 4.500 
               
               
                 708 
                 AVEAqPLLGA 
                 4.500 
               
               
                 427 
                 RAEGlPALRL 
                 4.500 
               
               
                 1097 
                 RPELqFFPLR 
                 4.500 
               
               
                 1290 
                 MEETgDMMPK 
                 4.500 
               
               
                 637 
                 EVEVeELLPL 
                 4.500 
               
               
                 1943 
                 KVEAeFELLT 
                 4.500 
               
               
                 1543 
                 ILELsISLPA 
                 4.500 
               
               
                 197 
                 DVELsGVMFS 
                 4.500 
               
               
                 947 
                 KLELfLRLGL 
                 4.500 
               
               
                 1379 
                 DSDGvNLISM 
                 3.750 
               
               
                 981 
                 SSLHrDDFSY 
                 3.750 
               
               
                 399 
                 CSDIeKNLLL 
                 3.750 
               
               
                 521 
                 ISHPgRAAGF 
                 3.000 
               
               
                 1906 
                 AQEAqAGKKK 
                 2.700 
               
               
                 127 
                 WSEEdFGAPI 
                 2.700 
               
               
                 786 
                 LQEVeRLQRK 
                 2.700 
               
               
                 1725 
                 DIKPrQPISY 
                 2.500 
               
               
                 1576 
                 HIDLeNRFYS 
                 2.500 
               
               
                 1747 
                 VLDDeNPLTG 
                 2.500 
               
               
                 2011 
                 LTVFlLLVFY 
                 2.500 
               
               
                 1117 
                 AAFQlIELDY 
                 2.500 
               
               
                 1930 
                 FTDMgGNVYI 
                 2.500 
               
               
                 40 
                 EADIgELFRW 
                 2.500 
               
               
                 193 
                 DDEPdVELSG 
                 2.250 
               
               
                 1786 
                 TGEGnFNWRF 
                 2.250 
               
               
                 703 
                 GTEGaAVEAQ 
                 2.250 
               
               
                 38 
                 GPEAdIGELF 
                 2.250 
               
               
                 585 
                 EPEPpQAQQG 
                 2.250 
               
               
                 1057 
                 QEEPpLVIIN 
                 2.250 
               
               
                 1502 
                 NLAPaDPNGK 
                 2.000 
               
               
                 938 
                 GAAPgEVCAK 
                 2.000 
               
               
                 235 
                 VLEAqKLVGV 
                 1.800 
               
               
                 1029 
                 VLEQtLSPLW 
                 1.800 
               
               
                 1821 
                 EAEFrQPAVL 
                 1.800 
               
               
                 626 
                 GAEGpEIPRA 
                 1.800 
               
               
                 1392 
                 IQDQgEAEVK 
                 1.500 
               
               
                 267 
                 TSCPfYNEYF 
                 1.500 
               
               
                 1360 
                 LQELqGQHNF 
                 1.350 
               
               
                 1754 
                 LTGEmSSDIY 
                 1.250 
               
               
                 340 
                 RMDLgIILDQ 
                 1.250 
               
               
                 1344 
                 IPDPeELDWG 
                 1.250 
               
               
                 266 
                 GTSCpFYNEY 
                 1.250 
               
               
                 217 
                 HGDPfQVSRA 
                 1.250 
               
               
                 1659 
                 SGDPeEAQAL 
                 1.250 
               
               
                 1777 
                 ETDVhFNSLT 
                 1.250 
               
               
                 1572 
                 IGEThIDLEN 
                 1.125 
               
               
                 629 
                 GPEIpRAMEV 
                 1.125 
               
               
                 181 
                 ENELeLELEQ 
                 1.125 
               
               
                 1755 
                 TGEMsSDIYV 
                 1.125 
               
               
                 1466 
                 YPESeAVLFS 
                 1.125 
               
               
                 1661 
                 DPEEaQALLV 
                 1.125 
               
               
                 1112 
                 AGELiAAFQL 
                 1.125 
               
               
                 620 
                 HLDAsPGAEG 
                 1.000 
               
               
                 841 
                 LVHSlNLLAK 
                 1.000 
               
               
                 1313 
                 EAGIsRQLLK 
                 1.000 
               
               
                 1481 
                 RGIPqNRPIK 
                 1.000 
               
               
                 949 
                 ELFLrLGLGK 
                 1.000 
               
               
                 1683 
                 QLVPeHVETR 
                 1.000 
               
               
                 868 
                 VALAkKLLAK 
                 1.000 
               
               
                 191 
                 DLDDePDVEL 
                 1.000 
               
               
                 315 
                 GLTPpSPKAF 
                 1.000 
               
               
                 1159 
                 NVCPvLREFR 
                 1.000 
               
               
                 1349 
                 ELDWgSKYYA 
                 1.000 
               
               
                 1121 
                 LIELdYSGRL 
                 0.900 
               
               
                 917 
                 VVEEeRGRDC 
                 0.900 
               
               
                 1927 
                 DLEFtDMGGN 
                 0.900 
               
               
                 1578 
                 DLENrFYSHH 
                 0.900 
               
               
                 689 
                 RLEEqLGRGS 
                 0.900 
               
               
                 725 
                 EEELgTHAQR 
                 0.900 
               
               
                 1952 
                 TVEEaEKRPV 
                 0.900 
               
               
                 183 
                 ELELeLEQDL 
                 0.900 
               
               
                 291 
                 LLEItVSGVG 
                 0.900 
               
               
                 1184 
                 LLEVeQPQVV 
                 0.900 
               
               
                 471 
                 SAEAaAPEWN 
                 0.900 
               
               
                 1848 
                 SLELqLPDMV 
                 0.900 
               
               
                 1164 
                 LREFrVEVLF 
                 0.900 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE X 
               
             
            
               
                   
               
               
                 158P3D2v.16, ORF: 65-6175, Frame +2, A0201-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 2010 
                 LLTVFLLLV 
                 1033.404 
               
               
                 2005 
                 LLLLVLLTV 
                 1006.209 
               
               
                 1490 
                 KLLVRVYVV 
                 849.359 
               
               
                 451 
                 VLVEPYVRV 
                 727.166 
               
               
                 873 
                 KLLAKLRFL 
                 699.907 
               
               
                 1010 
                 GLSDPFARV 
                 541.810 
               
               
                 1994 
                 FIWRRYWRT 
                 440.113 
               
               
                 571 
                 LLLAVSMQV 
                 437.482 
               
               
                 658 
                 LLFGVLFEA 
                 416.858 
               
               
                 2014 
                 FLLLVFYTI 
                 337.376 
               
               
                 98 
                 ALVDENLQV 
                 285.163 
               
               
                 445 
                 ALHDQRVLV 
                 285.163 
               
               
                 839 
                 KLLVHSLNL 
                 276.643 
               
               
                 2009 
                 VLLTVFLLL 
                 255.302 
               
               
                 2007 
                 LLVLLTVFL 
                 199.738 
               
               
                 895 
                 WMLSGQRRV 
                 170.990 
               
               
                 1155 
                 SLPPNVCPV 
                 159.970 
               
               
                 2008 
                 LVLLTVFLL 
                 156.843 
               
               
                 1984 
                 FVNPLKTFV 
                 153.971 
               
               
                 971 
                 LLPEPSAGL 
                 148.896 
               
               
                 1163 
                 VLREFRVEV 
                 140.004 
               
               
                 2004 
                 VLLLLVLLT 
                 107.808 
               
               
                 286 
                 RLQDLLLEI 
                 98.381 
               
               
                 1845 
                 FLGSLELQL 
                 98.267 
               
               
                 650 
                 VLAPCEDFL 
                 97.872 
               
               
                 1243 
                 YLQPPLSIL 
                 92.666 
               
               
                 234 
                 TVLEAQKLV 
                 92.322 
               
               
                 840 
                 LLVHSLNLL 
                 83.527 
               
               
                 1332 
                 GLLNQGPGL 
                 79.041 
               
               
                 435 
                 RLGLLGSLV 
                 69.552 
               
               
                 86 
                 QLQNAGHLV 
                 69.552 
               
               
                 103 
                 NLQVSPIQV 
                 69.552 
               
               
                 1710 
                 WIDIFPQDV 
                 66.867 
               
               
                 781 
                 RLDQGLQEV 
                 63.988 
               
               
                 1171 
                 VLFWGLRGL 
                 61.810 
               
               
                 2 
                 ALTVSVQRL 
                 49.134 
               
               
                 510 
                 ALATHVPDL 
                 49.134 
               
               
                 79 
                 TLVISLQQL 
                 49.134 
               
               
                 874 
                 LLAKLRFLA 
                 48.984 
               
               
                 1041 
                 LLVFEQLIV 
                 48.478 
               
               
                 1746 
                 VVLDDENPL 
                 48.205 
               
               
                 437 
                 GLLGSLVRA 
                 42.278 
               
               
                 1003 
                 VLAADDSGL 
                 36.316 
               
               
                 847 
                 LLAKQGLRL 
                 36.316 
               
               
                 572 
                 LLAVSMQVL 
                 34.246 
               
               
                 553 
                 SLQGLNEGV 
                 34.080 
               
               
                 406 
                 LLLPRGVPA 
                 31.249 
               
               
                 1635 
                 KVGSKVFLT 
                 30.444 
               
               
                 1756 
                 GEMSSDIYV 
                 27.521 
               
               
                 1739 
                 VIWNTEDVV 
                 27.109 
               
               
                 2003 
                 LVLLLLVLL 
                 27.042 
               
               
                 802 
                 AQLKQALEV 
                 26.092 
               
               
                 1464 
                 LIYPESEAV 
                 25.492 
               
               
                 1463 
                 FLIYPESEA 
                 22.853 
               
               
                 2021 
                 TIPGQISQV 
                 21.996 
               
               
                 499 
                 QLRDDAPLV 
                 21.672 
               
               
                 1082 
                 ALAAPRVKL 
                 21.362 
               
               
                 430 
                 GLPALRLGL 
                 21.362 
               
               
                 1747 
                 VLDDENPLT 
                 20.776 
               
               
                 1571 
                 LIGETHIDL 
                 20.473 
               
               
                 2002 
                 TLVLLLLVL 
                 20.145 
               
               
                 931 
                 SLMLTAPGA 
                 18.382 
               
               
                 1701 
                 GLLQGSLHM 
                 18.382 
               
               
                 1033 
                 TLSPLWDEL 
                 17.795 
               
               
                 291 
                 LLEITVSGV 
                 17.405 
               
               
                 1306 
                 SLDPFLAEA 
                 17.368 
               
               
                 438 
                 LLGSLVRAL 
                 16.705 
               
               
                 549 
                 GLRDSLQGL 
                 15.310 
               
               
                 1531 
                 YIPKQLNPI 
                 15.177 
               
               
                 225 
                 RAQDFQVGV 
                 15.050 
               
               
                 643 
                 LLPLPENVL 
                 14.890 
               
               
                 498 
                 LQLRDDAPL 
                 13.624 
               
               
                 1018 
                 VLISTQCQT 
                 12.668 
               
               
                 1244 
                 LQPPLSILV 
                 11.988 
               
               
                 63 
                 VQVVNCSRV 
                 11.988 
               
               
                 1224 
                 VVFKDTAPL 
                 11.757 
               
               
                 1938 
                 YILTGKVEA 
                 11.626 
               
               
                 60 
                 CLSVQVVNC 
                 11.426 
               
               
                 335 
                 RIGTFRMDL 
                 11.162 
               
               
                 1040 
                 ELLVFEQLI 
                 11.001 
               
               
                 184 
                 LELELEQDL 
                 10.712 
               
               
                 328 
                 TLPFMATRI 
                 10.433 
               
               
                 1703 
                 LQGSLHMWI 
                 9.890 
               
               
                 1055 
                 HLQEEPPLV 
                 9.696 
               
               
                 215 
                 LAHGDPFQV 
                 9.525 
               
               
                 642 
                 ELLPLPENV 
                 9.457 
               
               
                 1852 
                 QLPDMVRGA 
                 9.370 
               
               
                 1261 
                 TVLVGSHIV 
                 9.232 
               
               
                 1113 
                 GELIAAFQL 
                 8.914 
               
               
                 1614 
                 ILAGLCQRC 
                 8.446 
               
               
                 1639 
                 KVFLTPPET 
                 8.444 
               
               
                 2001 
                 RTLVLLLLV 
                 8.221 
               
               
                 1193 
                 VLEVAGQGV 
                 7.567 
               
               
                 1184 
                 LLEVEQPQV 
                 7.567 
               
               
                 1683 
                 QLVPEHVET 
                 7.452 
               
               
                 654 
                 CEDFLLFGV 
                 7.216 
               
               
                 1557 
                 TVAVFDHDL 
                 7.103 
               
               
                 638 
                 VEVEELLPL 
                 6.659 
               
               
                 867 
                 KVALAKKLL 
                 6.542 
               
               
                 888 
                 PLPDVLVWM 
                 5.669 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XI 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A0201-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 290 
                 LLLEiTVSGV 
                 4125.456 
               
               
                 2009 
                 VLLTvFLLLV 
                 3823.593 
               
               
                 276 
                 FLFEfHDTRL 
                 1490.711 
               
               
                 657 
                 FLLFgVLFEA 
                 1127.969 
               
               
                 2004 
                 VLLLlVLLTV 
                 1006.209 
               
               
                 2006 
                 LLLVlLTVFL 
                 739.032 
               
               
                 839 
                 KLLVhSLNLL 
                 636.279 
               
               
                 2007 
                 LLVLlTVFLL 
                 484.457 
               
               
                 214 
                 ALAHgDPFQV 
                 403.402 
               
               
                 880 
                 FLAEePQPPL 
                 402.895 
               
               
                 873 
                 KLLAkLRFLA 
                 373.146 
               
               
                 1243 
                 YLQPpLSILV 
                 319.939 
               
               
                 1801 
                 YLPTeREVSV 
                 319.939 
               
               
                 461 
                 FLGQeGETSV 
                 319.939 
               
               
                 570 
                 RLLLaVSMQV 
                 257.342 
               
               
                 650 
                 VLAPcEDFLL 
                 210.633 
               
               
                 1319 
                 QLLKpPLKKL 
                 181.794 
               
               
                 2012 
                 TVFLlLVFYT 
                 177.011 
               
               
                 1178 
                 GLGRvHLLEV 
                 159.970 
               
               
                 1192 
                 VVLEvAGQGV 
                 156.947 
               
               
                 1463 
                 FLIYpESEAV 
                 156.770 
               
               
                 571 
                 LLLAvSMQVL 
                 126.710 
               
               
                 1702 
                 LLQGsLHMWI 
                 97.547 
               
               
                 846 
                 NLLAkQGLRL 
                 79.041 
               
               
                 1986 
                 NPLKtFVFFI 
                 70.254 
               
               
                 1549 
                 SLPAeTELTV 
                 69.552 
               
               
                 1028 
                 RVLEqTLSPL 
                 65.219 
               
               
                 241 
                 LVGVnINPYV 
                 56.902 
               
               
                 869 
                 ALAKkLLAKL 
                 49.134 
               
               
                 2002 
                 TLVLlLLVLL 
                 49.134 
               
               
                 810 
                 VLVAgSRQFC 
                 46.451 
               
               
                 498 
                 LQLRdDAPLV 
                 44.356 
               
               
                 658 
                 LLFGvLFEAT 
                 43.639 
               
               
                 556 
                 GLNEgVGQGI 
                 42.774 
               
               
                 1734 
                 YELRvVIWNT 
                 42.542 
               
               
                 65 
                 VVNCsRVFSL 
                 42.390 
               
               
                 1994 
                 FIWRrYWRTL 
                 38.130 
               
               
                 437 
                 GLLGsLVRAL 
                 36.359 
               
               
                 1183 
                 HLLEvEQPQV 
                 35.874 
               
               
                 760 
                 SCWEdHTWRL 
                 35.591 
               
               
                 222 
                 QVSRaQDFQV 
                 35.298 
               
               
                 450 
                 RVLVePYVRV 
                 33.776 
               
               
                 888 
                 PLPDvLVWML 
                 33.239 
               
               
                 157 
                 AQLErRAVAL 
                 32.857 
               
               
                 1010 
                 GLSDpFARVL 
                 27.292 
               
               
                 777 
                 KVAErLDQGL 
                 26.823 
               
               
                 85 
                 QQLQnAGHLV 
                 26.092 
               
               
                 564 
                 GIWFrGRLLL 
                 24.380 
               
               
                 203 
                 VMFSpLKSRA 
                 22.569 
               
               
                 2008 
                 LVLLtVFLLL 
                 22.339 
               
               
                 1944 
                 VEAEfELLTV 
                 21.680 
               
               
                 497 
                 RLQLrDDAPL 
                 21.362 
               
               
                 844 
                 SLNLlAKQGL 
                 21.362 
               
               
                 1809 
                 SVWRrSGPFA 
                 19.844 
               
               
                 991 
                 FQLRaHLYQA 
                 19.718 
               
               
                 1491 
                 LLVRvYVVKA 
                 19.425 
               
               
                 649 
                 NVLApCEDFL 
                 18.639 
               
               
                 405 
                 NLLLpRGVPA 
                 18.382 
               
               
                 1455 
                 LVGKfKGSFL 
                 17.477 
               
               
                 235 
                 VLEAqKLVGV 
                 17.405 
               
               
                 1739 
                 VIWNtEDVVL 
                 16.993 
               
               
                 1746 
                 VVLDdENPLT 
                 16.816 
               
               
                 847 
                 LLAKqGLRLL 
                 16.705 
               
               
                 1795 
                 FVFRfDYLPT 
                 16.647 
               
               
                 1535 
                 QLNPiFGEIL 
                 16.308 
               
               
                 1825 
                 RQPAvLVLQV 
                 16.219 
               
               
                 424 
                 RLYRaEGLPA 
                 15.898 
               
               
                 1305 
                 KSLDpFLAEA 
                 15.049 
               
               
                 1833 
                 QVWDyDRISA 
                 14.793 
               
               
                 1668 
                 LLVLrRWQEM 
                 14.358 
               
               
                 286 
                 RLQDlLLEIT 
                 14.118 
               
               
                 295 
                 TVSGvGVTSV 
                 13.997 
               
               
                 12 
                 GLTGtHDRQV 
                 13.910 
               
               
                 352 
                 GQFYqRWVPL 
                 13.624 
               
               
                 1831 
                 VLQVwDYDRI 
                 13.036 
               
               
                 1018 
                 VLIStQCQTT 
                 12.668 
               
               
                 1639 
                 KVFLtPPETL 
                 11.861 
               
               
                 530 
                 FNPTfGPAWV 
                 11.487 
               
               
                 1115 
                 LIAAfQLIEL 
                 11.485 
               
               
                 1082 
                 ALAApRVKLM 
                 11.426 
               
               
                 1033 
                 TLSPlWDELL 
                 10.468 
               
               
                 908 
                 IPAQdVLFSV 
                 10.296 
               
               
                 2020 
                 YTIPgQISQV 
                 10.220 
               
               
                 1427 
                 FEDWlNVFPL 
                 10.196 
               
               
                 1047 
                 LIVDgRREHL 
                 10.032 
               
               
                 245 
                 NINPyVAVQV 
                 9.563 
               
               
                 143 
                 IIPNvGFQEL 
                 9.488 
               
               
                 2003 
                 LVLLlLVLLT 
                 9.433 
               
               
                 1771 
                 LEHDkQETDV 
                 9.426 
               
               
                 243 
                 GVNInPYVAV 
                 9.129 
               
               
                 453 
                 VEPYvRVSFL 
                 8.933 
               
               
                 175 
                 GQQDdEENEL 
                 8.880 
               
               
                 1155 
                 SLPPnVCPVL 
                 8.759 
               
               
                 86 
                 QLQNaGHLVL 
                 8.759 
               
               
                 1676 
                 EMPGfGIQLV 
                 8.665 
               
               
                 832 
                 ALDRcRGKLL 
                 8.545 
               
               
                 643 
                 LLPLpENVLA 
                 8.446 
               
               
                 840 
                 LLVHsLNLLA 
                 8.446 
               
               
                 1040 
                 ELLVfEQLIV 
                 7.913 
               
               
                 72 
                 FSLRpLGTLV 
                 7.727 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XII 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A3-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1319 
                 QLLKPPLKK 
                 90.000 
               
               
                 276 
                 FLFEFHDTR 
                 90.000 
               
               
                 1089 
                 KLMEDPYQR 
                 81.000 
               
               
                 869 
                 ALAKKLLAK 
                 60.000 
               
               
                 858 
                 GLRRRNVQK 
                 60.000 
               
               
                 1829 
                 VLVLQVWDY 
                 54.000 
               
               
                 1989 
                 KTFVFFIWR 
                 54.000 
               
               
                 1491 
                 LLVRVYVVK 
                 45.000 
               
               
                 1099 
                 ELQFFPLRK 
                 36.000 
               
               
                 1831 
                 VLQVWDYDR 
                 36.000 
               
               
                 1757 
                 EMSSDIYVK 
                 27.000 
               
               
                 1430 
                 WLNVFPLYR 
                 24.000 
               
               
                 203 
                 VMFSPLKSR 
                 22.500 
               
               
                 304 
                 VLQRRGDEK 
                 20.000 
               
               
                 953 
                 RLGLGKQAK 
                 20.000 
               
               
                 1950 
                 LLTVEEAEK 
                 20.000 
               
               
                 23 
                 LTFRGFTQK 
                 15.000 
               
               
                 2014 
                 FLLLVFYTI 
                 12.150 
               
               
                 982 
                 SLHRDDFSY 
                 12.000 
               
               
                 1310 
                 FLAEAGISR 
                 12.000 
               
               
                 658 
                 LLFGVLFEA 
                 10.125 
               
               
                 599 
                 RLTRKKKKK 
                 10.000 
               
               
                 1761 
                 DIYVKSWVK 
                 9.000 
               
               
                 1706 
                 SLHMWIDIF 
                 9.000 
               
               
                 1419 
                 SLKEEFNHF 
                 9.000 
               
               
                 1490 
                 KLLVRVYVV 
                 8.100 
               
               
                 2009 
                 VLLTVFLLL 
                 8.100 
               
               
                 2028 
                 QVIFRPLHK 
                 6.000 
               
               
                 1064 
                 IINVFDHNK 
                 6.000 
               
               
                 232 
                 GVTVLEAQK 
                 6.000 
               
               
                 1482 
                 GIPQNRPIK 
                 6.000 
               
               
                 560 
                 GVGQGIWFR 
                 5.400 
               
               
                 839 
                 KLLVHSLNL 
                 5.400 
               
               
                 2006 
                 LLLVLLTVF 
                 4.500 
               
               
                 768 
                 RLQSSNCVR 
                 4.000 
               
               
                 852 
                 GLRLLRGLR 
                 3.600 
               
               
                 1864 
                 ELCSVQLAR 
                 3.600 
               
               
                 1850 
                 ELQLPDMVR 
                 3.600 
               
               
                 430 
                 GLPALRLGL 
                 3.600 
               
               
                 2024 
                 GQISQVIFR 
                 3.240 
               
               
                 1767 
                 WVKGLEHDK 
                 3.000 
               
               
                 1036 
                 PLWDELLVF 
                 3.000 
               
               
                 2012 
                 TVFLLLVFY 
                 3.000 
               
               
                 1399 
                 EVKGTVSPK 
                 2.700 
               
               
                 1623 
                 GLPAPEYRA 
                 2.700 
               
               
                 1332 
                 GLLNQGPGL 
                 2.700 
               
               
                 2002 
                 TLVLLLLVL 
                 2.700 
               
               
                 549 
                 GLRDSLQGL 
                 2.700 
               
               
                 300 
                 GVTSVLQRR 
                 2.700 
               
               
                 1010 
                 GLSDPFARV 
                 2.700 
               
               
                 1932 
                 DMGGNVYIL 
                 2.430 
               
               
                 1247 
                 PLSILVIER 
                 2.400 
               
               
                 595 
                 STLSRLTRK 
                 2.250 
               
               
                 1243 
                 YLQPPLSIL 
                 2.025 
               
               
                 437 
                 GLLGSLVRA 
                 2.025 
               
               
                 327 
                 QTLPFMATR 
                 2.025 
               
               
                 1880 
                 NLFRCRRLR 
                 2.000 
               
               
                 596 
                 TLSRLTRKK 
                 2.000 
               
               
                 2 
                 ALTVSVQRL 
                 1.800 
               
               
                 635 
                 AMEVEVEEL 
                 1.800 
               
               
                 1701 
                 GLLQGSLHM 
                 1.800 
               
               
                 2010 
                 LLTVFLLLV 
                 1.800 
               
               
                 450 
                 RVLVEPYVR 
                 1.800 
               
               
                 846 
                 NLLAKQGLR 
                 1.800 
               
               
                 1684 
                 LVPEHVETR 
                 1.800 
               
               
                 510 
                 ALATHVPDL 
                 1.800 
               
               
                 286 
                 RLQDLLLEI 
                 1.800 
               
               
                 864 
                 VQKKVALAK 
                 1.800 
               
               
                 899 
                 GQRRVAWAR 
                 1.620 
               
               
                 1676 
                 EMPGFGIQL 
                 1.620 
               
               
                 1987 
                 PLKTFVFFI 
                 1.620 
               
               
                 141 
                 ELIIPNVGF 
                 1.350 
               
               
                 315 
                 GLTPPSPKA 
                 1.350 
               
               
                 1267 
                 HIVPHMLRF 
                 1.350 
               
               
                 1145 
                 PLVEPHSGR 
                 1.350 
               
               
                 971 
                 LLPEPSAGL 
                 1.350 
               
               
                 840 
                 LLVHSLNLL 
                 1.350 
               
               
                 79 
                 TLVISLQQL 
                 1.350 
               
               
                 2005 
                 LLLLVLLTV 
                 1.350 
               
               
                 1306 
                 SLDPFLAEA 
                 1.350 
               
               
                 1535 
                 QLNPIFGEI 
                 1.215 
               
               
                 284 
                 RLRLQDLLL 
                 1.200 
               
               
                 1845 
                 FLGSLELQL 
                 1.200 
               
               
                 847 
                 LLAKQGLRL 
                 1.200 
               
               
                 1889 
                 GWWPVVKLK 
                 1.013 
               
               
                 1320 
                 LLKPPLKKL 
                 1.012 
               
               
                 854 
                 RLLRGLRRR 
                 0.900 
               
               
                 1526 
                 DTKERYIPK 
                 0.900 
               
               
                 1029 
                 VLEQTLSPL 
                 0.900 
               
               
                 643 
                 LLPLPENVL 
                 0.900 
               
               
                 420 
                 RLRVRLYRA 
                 0.900 
               
               
                 571 
                 LLLAVSMQV 
                 0.900 
               
               
                 650 
                 VLAPCEDFL 
                 0.900 
               
               
                 1033 
                 TLSPLWDEL 
                 0.900 
               
               
                 939 
                 AAPGEVCAK 
                 0.900 
               
               
                 337 
                 GTFRMDLGI 
                 0.900 
               
               
                 1318 
                 RQLLKPPLK 
                 0.900 
               
               
                 992 
                 QLRAHLYQA 
                 0.900 
               
               
                 996 
                 HLYQARGVL 
                 0.900 
               
               
                 331 
                 FMATRIGTF 
                 0.900 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XIII 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A3-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 858 
                 GLRRrNVQKK 
                 180.000 
               
               
                 22 
                 KLTFrGFTQK 
                 180.000 
               
               
                 1490 
                 KLLVrVYVVK 
                 135.000 
               
               
                 1886 
                 RLRGwWPVVK 
                 90.000 
               
               
                 949 
                 ELFLrLGLGK 
                 60.000 
               
               
                 1472 
                 VLFSePQISR 
                 60.000 
               
               
                 1989 
                 KTFVfFIWRR 
                 40.500 
               
               
                 240 
                 KLVGvNINPY 
                 40.500 
               
               
                 785 
                 GLQEvERLQR 
                 36.000 
               
               
                 1502 
                 NLAPaDPNGK 
                 30.000 
               
               
                 913 
                 VLFSvVEEER 
                 30.000 
               
               
                 714 
                 LLGArPEEEK 
                 30.000 
               
               
                 1683 
                 QLVPeHVETR 
                 27.000 
               
               
                 852 
                 GLRLlRGLRR 
                 24.000 
               
               
                 1412 
                 TLKIyNRSLK 
                 20.000 
               
               
                 768 
                 RLQSsNCVRK 
                 20.000 
               
               
                 1895 
                 KLKEaEDVER 
                 18.000 
               
               
                 596 
                 TLSRlTRKKK 
                 10.000 
               
               
                 1949 
                 ELLTvEEAEK 
                 9.000 
               
               
                 1063 
                 VIINvFDHNK 
                 9.000 
               
               
                 1120 
                 QLIElDYSGR 
                 9.000 
               
               
                 1296 
                 MMPKgPQGQK 
                 9.000 
               
               
                 2007 
                 LLVLlTVFLL 
                 8.100 
               
               
                 826 
                 TMTRpNALDR 
                 8.000 
               
               
                 435 
                 RLGLlGSLVR 
                 8.000 
               
               
                 298 
                 GVGVtSVLQR 
                 7.200 
               
               
                 2010 
                 LLTVfLLLVF 
                 6.000 
               
               
                 510 
                 ALAThVPDLR 
                 6.000 
               
               
                 863 
                 NVQKkVALAK 
                 6.000 
               
               
                 407 
                 LLPRgVPAER 
                 6.000 
               
               
                 1218 
                 LVRHlTVVFK 
                 6.000 
               
               
                 441 
                 SLVRaLHDQR 
                 6.000 
               
               
                 331 
                 FMATrIGTFR 
                 6.000 
               
               
                 841 
                 LVHSlNLLAK 
                 6.000 
               
               
                 982 
                 SLHRdDFSYF 
                 6.000 
               
               
                 1828 
                 AVLVlQVWDY 
                 5.400 
               
               
                 1830 
                 LVLQvWDYDR 
                 5.400 
               
               
                 315 
                 GLTPpSPKAF 
                 4.500 
               
               
                 276 
                 FLFEfHDTRL 
                 4.500 
               
               
                 1939 
                 ILTGkVEAEF 
                 4.500 
               
               
                 2005 
                 LLLLvLLTVF 
                 4.500 
               
               
                 1454 
                 HLVGkFKGSF 
                 4.050 
               
               
                 839 
                 KLLVhSLNLL 
                 4.050 
               
               
                 556 
                 GLNEgVGQGI 
                 4.050 
               
               
                 1950 
                 LLTVeEAEKR 
                 4.000 
               
               
                 1078 
                 FLGRaLAAPR 
                 4.000 
               
               
                 992 
                 QLRAhLYQAR 
                 4.000 
               
               
                 346 
                 ILDQpDGQFY 
                 4.000 
               
               
                 947 
                 KLELfLRLGL 
                 3.600 
               
               
                 411 
                 GVPAeRPWAR 
                 3.600 
               
               
                 303 
                 SVLQrRGDEK 
                 3.000 
               
               
                 2009 
                 VLLTvFLLLV 
                 2.700 
               
               
                 938 
                 GAAPgEVCAK 
                 2.700 
               
               
                 1535 
                 QLNPiFGEIL 
                 2.700 
               
               
                 650 
                 VLAPcEDFLL 
                 2.700 
               
               
                 1491 
                 LLVRvYVVKA 
                 2.700 
               
               
                 1318 
                 RQLLkPPLKK 
                 2.700 
               
               
                 1200 
                 GVESeVLASY 
                 2.700 
               
               
                 873 
                 KLLAkLRFLA 
                 2.700 
               
               
                 1055 
                 HLQEePPLVI 
                 2.700 
               
               
                 1163 
                 VLREfRVEVL 
                 2.700 
               
               
                 1175 
                 GLRGlGRVHL 
                 2.700 
               
               
                 1033 
                 TLSPlWDELL 
                 2.700 
               
               
                 658 
                 LLFGvLFEAT 
                 2.250 
               
               
                 202 
                 GVMFsPLKSR 
                 2.025 
               
               
                 657 
                 FLLFgVLFEA 
                 2.025 
               
               
                 424 
                 RLYRaEGLPA 
                 2.000 
               
               
                 893 
                 LVWMlSGQRR 
                 2.000 
               
               
                 846 
                 NLLAkQGLRL 
                 1.800 
               
               
                 892 
                 VLVWmLSGQR 
                 1.800 
               
               
                 73 
                 SLRPlGTLVI 
                 1.800 
               
               
                 1852 
                 QLPDmVRGAR 
                 1.800 
               
               
                 2027 
                 SQVIfRPLHK 
                 1.800 
               
               
                 266 
                 GTSCpFYNEY 
                 1.800 
               
               
                 1178 
                 GLGRvHLLEV 
                 1.800 
               
               
                 1409 
                 AVATlKIYNR 
                 1.800 
               
               
                 1702 
                 LLQGsLHMWI 
                 1.800 
               
               
                 564 
                 GIWFrGRLLL 
                 1.800 
               
               
                 807 
                 ALEVlVAGSR 
                 1.800 
               
               
                 880 
                 FLAEePQPPL 
                 1.350 
               
               
                 1639 
                 KVFLtPPETL 
                 1.350 
               
               
                 571 
                 LLLAvSMQVL 
                 1.350 
               
               
                 290 
                 LLLEiTVSGV 
                 1.350 
               
               
                 1399 
                 EVKGtVSPKK 
                 1.350 
               
               
                 45 
                 ELFRwPHYGA 
                 1.350 
               
               
                 1701 
                 GLLQgSLHMW 
                 1.350 
               
               
                 1718 
                 VPAPpPVDIK 
                 1.350 
               
               
                 1155 
                 SLPPnVCPVL 
                 1.350 
               
               
                 2004 
                 VLLLlVLLTV 
                 1.350 
               
               
                 2002 
                 TLVLlLLVLL 
                 1.350 
               
               
                 437 
                 GLLGsLVRAL 
                 1.215 
               
               
                 1756 
                 GEMSsDIYVK 
                 1.215 
               
               
                 1617 
                 GLCQrCGLPA 
                 1.200 
               
               
                 1869 
                 QLARnGAGPR 
                 1.200 
               
               
                 485 
                 FVELfPPLTR 
                 1.200 
               
               
                 86 
                 QLQNaGHLVL 
                 1.200 
               
               
                 517 
                 DLRRiSHPGR 
                 1.200 
               
               
                 1319 
                 QLLKpPLKKL 
                 1.012 
               
               
                 1224 
                 VVFKdTAPLF 
                 1.000 
               
               
                 13 
                 LTGThDRQVK 
                 1.000 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XIV 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A1101-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 2028 
                 QVIFRPLHK 
                 6.000 
               
               
                 232 
                 GVTVLEAQK 
                 6.000 
               
               
                 450 
                 RVLVEPYVR 
                 3.600 
               
               
                 1318 
                 RQLLKPPLK 
                 2.700 
               
               
                 560 
                 GVGQGIWFR 
                 2.400 
               
               
                 1989 
                 KTFVFFIWR 
                 2.400 
               
               
                 23 
                 LTFRGFTQK 
                 2.000 
               
               
                 1767 
                 WVKGLEHDK 
                 2.000 
               
               
                 368 
                 RAGTKGFIK 
                 1.800 
               
               
                 595 
                 STLSRLTRK 
                 1.500 
               
               
                 953 
                 RLGLGKQAK 
                 1.200 
               
               
                 858 
                 GLRRRNVQK 
                 1.200 
               
               
                 864 
                 VQKKVALAK 
                 1.200 
               
               
                 1319 
                 QLLKPPLKK 
                 1.200 
               
               
                 300 
                 GVTSVLQRR 
                 1.200 
               
               
                 1482 
                 GIPQNRPIK 
                 1.200 
               
               
                 2024 
                 GQISQVIFR 
                 1.080 
               
               
                 1089 
                 KLMEDPYQR 
                 0.960 
               
               
                 869 
                 ALAKKLLAK 
                 0.800 
               
               
                 893 
                 LVWMLSGQR 
                 0.800 
               
               
                 816 
                 RQFCHGAER 
                 0.720 
               
               
                 899 
                 GQRRVAWAR 
                 0.720 
               
               
                 769 
                 LQSSNCVRK 
                 0.600 
               
               
                 1399 
                 EVKGTVSPK 
                 0.600 
               
               
                 599 
                 RLTRKKKKK 
                 0.600 
               
               
                 950 
                 LFLRLGLGK 
                 0.600 
               
               
                 797 
                 GPGACAQLK 
                 0.600 
               
               
                 607 
                 KARRDQTPK 
                 0.600 
               
               
                 1906 
                 AQEAQAGKK 
                 0.600 
               
               
                 1526 
                 DTKERYIPK 
                 0.600 
               
               
                 1491 
                 LLVRVYVVK 
                 0.600 
               
               
                 1761 
                 DIYVKSWVK 
                 0.480 
               
               
                 512 
                 ATHVPDLRR 
                 0.400 
               
               
                 1684 
                 LVPEHVETR 
                 0.400 
               
               
                 827 
                 MTRPNALDR 
                 0.400 
               
               
                 304 
                 VLQRRGDEK 
                 0.400 
               
               
                 1950 
                 LLTVEEAEK 
                 0.400 
               
               
                 62 
                 SVQVVNCSR 
                 0.400 
               
               
                 1064 
                 IINVFDHNK 
                 0.400 
               
               
                 251 
                 AVQVGGQRR 
                 0.400 
               
               
                 442 
                 LVRALHDQR 
                 0.400 
               
               
                 1729 
                 RQPISYELR 
                 0.360 
               
               
                 327 
                 QTLPFMATR 
                 0.300 
               
               
                 745 
                 FCLPLCHCK 
                 0.300 
               
               
                 1951 
                 LTVEEAEKR 
                 0.300 
               
               
                 1909 
                 AQAGKKKRK 
                 0.300 
               
               
                 1757 
                 EMSSDIYVK 
                 0.240 
               
               
                 786 
                 LQEVERLQR 
                 0.240 
               
               
                 591 
                 AQQGSTLSR 
                 0.240 
               
               
                 1099 
                 ELQFFPLRK 
                 0.240 
               
               
                 852 
                 GLRLLRGLR 
                 0.240 
               
               
                 1665 
                 AQALLVLRR 
                 0.240 
               
               
                 768 
                 RLQSSNCVR 
                 0.240 
               
               
                 821 
                 GAERRTMTR 
                 0.240 
               
               
                 626 
                 GAEGPEIPR 
                 0.240 
               
               
                 939 
                 AAPGEVCAK 
                 0.200 
               
               
                 1297 
                 MPKGPQGQK 
                 0.200 
               
               
                 1503 
                 LAPADPNGK 
                 0.200 
               
               
                 1935 
                 GNVYILTGK 
                 0.180 
               
               
                 1430 
                 WLNVFPLYR 
                 0.160 
               
               
                 1831 
                 VLQVWDYDR 
                 0.160 
               
               
                 1310 
                 FLAEAGISR 
                 0.160 
               
               
                 276 
                 FLFEFHDTR 
                 0.160 
               
               
                 26 
                 RGFTQKTRK 
                 0.120 
               
               
                 1965 
                 RKQPEPLEK 
                 0.120 
               
               
                 337 
                 GTFRMDLGI 
                 0.120 
               
               
                 376 
                 KVTLSVRAR 
                 0.120 
               
               
                 1798 
                 RFDYLPTER 
                 0.120 
               
               
                 1168 
                 RVEVLFWGL 
                 0.120 
               
               
                 427 
                 RAEGLPALR 
                 0.120 
               
               
                 1878 
                 RCNLFRCRR 
                 0.120 
               
               
                 243 
                 GVNINPYVA 
                 0.120 
               
               
                 902 
                 RVAWARIPA 
                 0.120 
               
               
                 1598 
                 EVDGYNAWR 
                 0.120 
               
               
                 945 
                 CAKLELFLR 
                 0.120 
               
               
                 164 
                 VALGRRLAR 
                 0.120 
               
               
                 1990 
                 TFVFFIWRR 
                 0.120 
               
               
                 1993 
                 FFIWRRYWR 
                 0.120 
               
               
                 846 
                 NLLAKQGLR 
                 0.120 
               
               
                 1613 
                 QILAGLCQR 
                 0.120 
               
               
                 661 
                 GVLFEATMI 
                 0.090 
               
               
                 1081 
                 RALAAPRVK 
                 0.090 
               
               
                 2008 
                 LVLLTVFLL 
                 0.090 
               
               
                 1062 
                 LVIINVFDH 
                 0.090 
               
               
                 2001 
                 RTLVLLLLV 
                 0.090 
               
               
                 787 
                 QEVERLQRK 
                 0.090 
               
               
                 1121 
                 LIELDYSGR 
                 0.080 
               
               
                 203 
                 VMFSPLKSR 
                 0.080 
               
               
                 760 
                 SCWEDHTWR 
                 0.080 
               
               
                 412 
                 VPAERPWAR 
                 0.080 
               
               
                 1410 
                 VATLKIYNR 
                 0.080 
               
               
                 1473 
                 LFSEPQISR 
                 0.080 
               
               
                 1212 
                 SPNFTELVR 
                 0.080 
               
               
                 349 
                 QPDGQFYQR 
                 0.080 
               
               
                 374 
                 FIKVTLSVR 
                 0.080 
               
               
                 1981 
                 FNWFVNPLK 
                 0.080 
               
               
                 1958 
                 KRPVGKGRK 
                 0.060 
               
               
                 1314 
                 AGISRQLLK 
                 0.060 
               
               
                 1889 
                 GWWPVVKLK 
                 0.060 
               
               
                 1087 
                 RVKLMEDPY 
                 0.060 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XV 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A1101-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1318 
                 RQLLkPPLKK 
                 5.400 
               
               
                 841 
                 LVHSlNLLAK 
                 4.000 
               
               
                 863 
                 NVQKkVALAK 
                 4.000 
               
               
                 303 
                 SVLQrRGDEK 
                 3.000 
               
               
                 298 
                 GVGVtSVLQR 
                 2.400 
               
               
                 411 
                 GVPAeRPWAR 
                 2.400 
               
               
                 1989 
                 KTFVfFIWRR 
                 2.400 
               
               
                 1218 
                 LVRHlTVVFK 
                 2.000 
               
               
                 2027 
                 SQVIfRPLHK 
                 1.800 
               
               
                 1490 
                 KLLVrVYVVK 
                 1.800 
               
               
                 1886 
                 RLRGwWPVVK 
                 1.200 
               
               
                 202 
                 GVMFsPLKSR 
                 1.200 
               
               
                 768 
                 RLQSsNCVRK 
                 1.200 
               
               
                 1168 
                 RVEVlFWGLR 
                 1.200 
               
               
                 858 
                 GLRRrNVQKK 
                 1.200 
               
               
                 22 
                 KLTFrGFTQK 
                 1.200 
               
               
                 1830 
                 LVLQvWDYDR 
                 1.200 
               
               
                 13 
                 LTGThDRQVK 
                 1.000 
               
               
                 893 
                 LVWMlSGQRR 
                 0.800 
               
               
                 1409 
                 AVATlKIYNR 
                 0.800 
               
               
                 163 
                 AVALgRRLAR 
                 0.800 
               
               
                 485 
                 FVELfPPLTR 
                 0.800 
               
               
                 816 
                 RQFChGAERR 
                 0.720 
               
               
                 1756 
                 GEMSsDIYVK 
                 0.720 
               
               
                 1063 
                 VIINvFDHNK 
                 0.600 
               
               
                 864 
                 VQKKvALAKK 
                 0.600 
               
               
                 938 
                 GAAPgEVCAK 
                 0.600 
               
               
                 868 
                 VALAkKLLAK 
                 0.600 
               
               
                 786 
                 LQEVeRLQRK 
                 0.600 
               
               
                 1392 
                 IQDQgEAEVK 
                 0.600 
               
               
                 1630 
                 RAGAvKVGSK 
                 0.600 
               
               
                 1399 
                 EVKGtVSPKK 
                 0.600 
               
               
                 785 
                 GLQEvERLQR 
                 0.480 
               
               
                 435 
                 RLGLlGSLVR 
                 0.480 
               
               
                 949 
                 ELFLrLGLGK 
                 0.480 
               
               
                 852 
                 GLRLlRGLRR 
                 0.480 
               
               
                 249 
                 YVAVqVGGQR 
                 0.400 
               
               
                 714 
                 LLGArPEEEK 
                 0.400 
               
               
                 1296 
                 MMPKgPQGQK 
                 0.400 
               
               
                 574 
                 AVSMqVLEGR 
                 0.400 
               
               
                 1412 
                 TLKIyNRSLK 
                 0.400 
               
               
                 1159 
                 NVCPvLREFR 
                 0.400 
               
               
                 1502 
                 NLAPaDPNGK 
                 0.400 
               
               
                 395 
                 APGHcSDIEK 
                 0.400 
               
               
                 256 
                 GQRRvTATQR 
                 0.360 
               
               
                 1472 
                 VLFSePQISR 
                 0.320 
               
               
                 1906 
                 AQEAqAGKKK 
                 0.300 
               
               
                 107 
                 SPIQvELDLK 
                 0.300 
               
               
                 1895 
                 KLKEaEDVER 
                 0.240 
               
               
                 1072 
                 KFGPpVFLGR 
                 0.240 
               
               
                 1874 
                 GAGPrCNLFR 
                 0.240 
               
               
                 1980 
                 SFNWfVNPLK 
                 0.200 
               
               
                 10 
                 LTGLtGTHDR 
                 0.200 
               
               
                 492 
                 LTRSlRLQLR 
                 0.200 
               
               
                 596 
                 TLSRlTRKKK 
                 0.200 
               
               
                 744 
                 YFCLpLCHCK 
                 0.200 
               
               
                 1519 
                 SAGReRQDTK 
                 0.200 
               
               
                 1626 
                 APEYrAGAVK 
                 0.200 
               
               
                 313 
                 AAGLtPPSPK 
                 0.200 
               
               
                 1785 
                 LTGEgNFNWR 
                 0.200 
               
               
                 1718 
                 VPAPpPVDIK 
                 0.200 
               
               
                 1405 
                 SPKKaVATLK 
                 0.200 
               
               
                 373 
                 GFIKvTLSVR 
                 0.180 
               
               
                 1612 
                 SQILaGLCQR 
                 0.180 
               
               
                 1949 
                 ELLTvEEAEK 
                 0.180 
               
               
                 1904 
                 REAQeAQAGK 
                 0.180 
               
               
                 1172 
                 LFWGlRGLGR 
                 0.160 
               
               
                 1992 
                 VFFIwRRYWR 
                 0.160 
               
               
                 913 
                 VLFSvVEEER 
                 0.160 
               
               
                 826 
                 TMTRpNALDR 
                 0.160 
               
               
                 595 
                 STLSrLTRKK 
                 0.150 
               
               
                 1964 
                 GRKQpEPLEK 
                 0.120 
               
               
                 944 
                 VCAKlELFLR 
                 0.120 
               
               
                 1290 
                 MEETgDMMPK 
                 0.120 
               
               
                 892 
                 VLVWmLSGQR 
                 0.120 
               
               
                 87 
                 LQNAgHLVLR 
                 0.120 
               
               
                 1621 
                 RCGLpAPEYR 
                 0.120 
               
               
                 867 
                 KVALaKKLLA 
                 0.120 
               
               
                 1494 
                 RVYVvKATNL 
                 0.120 
               
               
                 1313 
                 EAGIsRQLLK 
                 0.120 
               
               
                 1120 
                 QLIElDYSGR 
                 0.120 
               
               
                 326 
                 SQTLpFMATR 
                 0.120 
               
               
                 1639 
                 KVFLtPPETL 
                 0.120 
               
               
                 1097 
                 RPELqFFPLR 
                 0.120 
               
               
                 1683 
                 QLVPeHVETR 
                 0.120 
               
               
                 441 
                 SLVRaLHDQR 
                 0.120 
               
               
                 600 
                 LTRKkKKKAR 
                 0.100 
               
               
                 1481 
                 RGIPqNRPIK 
                 0.090 
               
               
                 857 
                 RGLRrRNVQK 
                 0.090 
               
               
                 2001 
                 RTLVlLLLVL 
                 0.090 
               
               
                 1002 
                 GVLAaDDSGL 
                 0.090 
               
               
                 1737 
                 RVVIwNTEDV 
                 0.090 
               
               
                 1028 
                 RVLEqTLSPL 
                 0.090 
               
               
                 450 
                 RVLVePYVRV 
                 0.090 
               
               
                 1251 
                 LVIErRAFGH 
                 0.090 
               
               
                 1398 
                 AEVKgTVSPK 
                 0.090 
               
               
                 407 
                 LLPRgVPAER 
                 0.080 
               
               
                 1950 
                 LLTVeEAEKR 
                 0.080 
               
               
                 510 
                 ALAThVPDLR 
                 0.080 
               
               
                 331 
                 FMATrIGTFR 
                 0.080 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XVI 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A24-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1355 
                 KYYASLQEL 
                 528.000 
               
               
                 1998 
                 RYWRTLVLL 
                 400.000 
               
               
                 1465 
                 IYPESEAVL 
                 360.000 
               
               
                 1495 
                 VYVVKATNL 
                 300.000 
               
               
                 1694 
                 LYHPHSPGL 
                 240.000 
               
               
                 989 
                 SYFQLRAHL 
                 240.000 
               
               
                 1601 
                 GYNAWRDAF 
                 150.000 
               
               
                 1794 
                 RFVFRFDYL 
                 72.000 
               
               
                 484 
                 SFVELFPPL 
                 51.840 
               
               
                 488 
                 LFPPLTRSL 
                 43.200 
               
               
                 1980 
                 SFNWFVNPL 
                 36.000 
               
               
                 277 
                 LFEFHDTRL 
                 30.000 
               
               
                 1423 
                 EFNHFEDWL 
                 30.000 
               
               
                 1640 
                 VFLTPPETL 
                 30.000 
               
               
                 228 
                 DFQVGVTVL 
                 30.000 
               
               
                 1214 
                 NFTELVRHL 
                 28.800 
               
               
                 533 
                 TFGPAWVPL 
                 24.000 
               
               
                 279 
                 EFHDTRLRL 
                 24.000 
               
               
                 1426 
                 HFEDWLNVF 
                 21.600 
               
               
                 353 
                 QFYQRWVPL 
                 20.000 
               
               
                 116 
                 KYQPPEGAT 
                 18.000 
               
               
                 1168 
                 RVEVLFWGL 
                 17.280 
               
               
                 656 
                 DFLLFGVLF 
                 15.000 
               
               
                 1983 
                 WFVNPLKTF 
                 15.000 
               
               
                 1094 
                 PYQRPELQF 
                 15.000 
               
               
                 162 
                 RAVALGRRL 
                 14.400 
               
               
                 1097 
                 RPELQFFPL 
                 14.400 
               
               
                 825 
                 RTMTRPNAL 
                 14.400 
               
               
                 1943 
                 KVEAEFELL 
                 14.400 
               
               
                 1107 
                 KGPWAAGEL 
                 13.200 
               
               
                 1322 
                 KPPLKKLPL 
                 12.000 
               
               
                 48 
                 RWPHYGAPL 
                 12.000 
               
               
                 873 
                 KLLAKLRFL 
                 12.000 
               
               
                 839 
                 KLLVHSLNL 
                 12.000 
               
               
                 862 
                 RNVQKKVAL 
                 12.000 
               
               
                 1225 
                 VFKDTAPLF 
                 12.000 
               
               
                 1177 
                 RGLGRVHLL 
                 12.000 
               
               
                 444 
                 RALHDQRVL 
                 12.000 
               
               
                 1299 
                 KGPQGQKSL 
                 12.000 
               
               
                 2026 
                 ISQVIFRPL 
                 10.080 
               
               
                 2009 
                 VLLTVFLLL 
                 10.080 
               
               
                 323 
                 AFHSQTLPF 
                 10.000 
               
               
                 1800 
                 DYLPTEREV 
                 9.900 
               
               
                 308 
                 RGDEKAAGL 
                 9.600 
               
               
                 686 
                 RAGRLEEQL 
                 9.600 
               
               
                 837 
                 RGKLLVHSL 
                 9.600 
               
               
                 144 
                 IPNVGFQEL 
                 9.504 
               
               
                 1888 
                 RGWWPVVKL 
                 8.800 
               
               
                 778 
                 VAERLDQGL 
                 8.640 
               
               
                 1746 
                 VVLDDENPL 
                 8.640 
               
               
                 1542 
                 EILELSISL 
                 8.640 
               
               
                 297 
                 SGVGVTSVL 
                 8.400 
               
               
                 107 
                 SPIQVELDL 
                 8.400 
               
               
                 503 
                 DAPLVDAAL 
                 8.400 
               
               
                 1156 
                 LPPNVCPVL 
                 8.400 
               
               
                 384 
                 RGDLPPPML 
                 8.000 
               
               
                 284 
                 RLRLQDLLL 
                 8.000 
               
               
                 867 
                 KVALAKKLL 
                 8.000 
               
               
                 335 
                 RIGTFRMDL 
                 8.000 
               
               
                 1963 
                 KGRKQPEPL 
                 8.000 
               
               
                 1548 
                 ISLPAETEL 
                 7.920 
               
               
                 233 
                 VTVLEAQKL 
                 7.920 
               
               
                 1242 
                 PYLQPPLSI 
                 7.500 
               
               
                 1596 
                 QYEVDGYNA 
                 7.500 
               
               
                 997 
                 LYQARGVLA 
                 7.500 
               
               
                 1733 
                 SYELRVVIW 
                 7.500 
               
               
                 105 
                 QVSPIQVEL 
                 7.392 
               
               
                 940 
                 APGEVCAKL 
                 7.392 
               
               
                 643 
                 LLPLPENVL 
                 7.200 
               
               
                 845 
                 LNLLAKQGL 
                 7.200 
               
               
                 1240 
                 EQPYLQPPL 
                 7.200 
               
               
                 1230 
                 APLFHPQDL 
                 7.200 
               
               
                 1243 
                 YLQPPLSIL 
                 7.200 
               
               
                 2007 
                 LLVLLTVFL 
                 7.200 
               
               
                 1841 
                 SANDFLGSL 
                 7.200 
               
               
                 840 
                 LLVHSLNLL 
                 7.200 
               
               
                 1146 
                 LVEPHSGRL 
                 7.200 
               
               
                 971 
                 LLPEPSAGL 
                 7.200 
               
               
                 2002 
                 TLVLLLLVL 
                 7.200 
               
               
                 851 
                 QGLRLLRGL 
                 7.200 
               
               
                 151 
                 ELEPGEAQL 
                 7.200 
               
               
                 881 
                 LAEEPQPPL 
                 7.200 
               
               
                 1661 
                 DPEEAQALL 
                 7.200 
               
               
                 430 
                 GLPALRLGL 
                 7.200 
               
               
                 1208 
                 SYRESPNFT 
                 7.200 
               
               
                 707 
                 AAVEAQPLL 
                 7.200 
               
               
                 79 
                 TLVISLQQL 
                 7.200 
               
               
                 2003 
                 LVLLLLVLL 
                 7.200 
               
               
                 761 
                 CWEDHTWRL 
                 7.200 
               
               
                 475 
                 AAPEWNEQL 
                 7.200 
               
               
                 2018 
                 VFYTIPGQI 
                 7.000 
               
               
                 398 
                 HCSDIEKNL 
                 6.720 
               
               
                 613 
                 TPKAVPQHL 
                 6.720 
               
               
                 134 
                 APIQDSFEL 
                 6.600 
               
               
                 38 
                 GPEADIGEL 
                 6.600 
               
               
                 1483 
                 IPQNRPIKL 
                 6.600 
               
               
                 635 
                 AMEVEVEEL 
                 6.600 
               
               
                 1033 
                 TLSPLWDEL 
                 6.336 
               
               
                 176 
                 QQDDEENEL 
                 6.336 
               
               
                 2019 
                 FYTIPGQIS 
                 6.000 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XVII 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, A24-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1998 
                 RYWRtLVLLL 
                 480.000 
               
               
                 1762 
                 IYVKsWVKGL 
                 300.000 
               
               
                 1694 
                 LYHPhSPGLL 
                 240.000 
               
               
                 1530 
                 RYIPkQLNPI 
                 216.000 
               
               
                 425 
                 LYRAeGLPAL 
                 200.000 
               
               
                 541 
                 LYGSpPGAGL 
                 200.000 
               
               
                 271 
                 FYNEyFLFEF 
                 198.000 
               
               
                 1415 
                 IYNRsLKEEF 
                 198.000 
               
               
                 1465 
                 IYPEsEAVLF 
                 180.000 
               
               
                 1836 
                 DYDRiSANDF 
                 120.000 
               
               
                 1844 
                 DFLGsLELQL 
                 36.000 
               
               
                 1242 
                 PYLQpPLSIL 
                 30.000 
               
               
                 338 
                 TFRMdLGIIL 
                 24.000 
               
               
                 71 
                 VFSLrPLGTL 
                 20.000 
               
               
                 1823 
                 EFRQpAVLVL 
                 20.000 
               
               
                 1094 
                 PYQRpELQFF 
                 18.000 
               
               
                 634 
                 RAMEvEVEEL 
                 15.840 
               
               
                 1790 
                 NFNWrFVFRF 
                 15.000 
               
               
                 2001 
                 RTLVlLLLVL 
                 14.400 
               
               
                 1861 
                 RGPElCSVQL 
                 14.400 
               
               
                 75 
                 RPLGtLVISL 
                 14.400 
               
               
                 947 
                 KLELfLRLGL 
                 14.400 
               
               
                 839 
                 KLLVhSLNLL 
                 14.400 
               
               
                 1028 
                 RVLEqTLSPL 
                 14.400 
               
               
                 131 
                 DFGApIQDSF 
                 14.000 
               
               
                 777 
                 KVAErLDQGL 
                 13.824 
               
               
                 1081 
                 RALAaPRVKL 
                 13.200 
               
               
                 2013 
                 VFLLlVFYTI 
                 12.600 
               
               
                 1727 
                 KPRQpISYEL 
                 12.320 
               
               
                 1878 
                 RCNLfRCRRL 
                 12.000 
               
               
                 427 
                 RAEGlPALRL 
                 12.000 
               
               
                 497 
                 RLQLrDDAPL 
                 12.000 
               
               
                 1733 
                 SYELrVVIWN 
                 10.500 
               
               
                 612 
                 QTPKaVPQHL 
                 10.080 
               
               
                 2008 
                 LVLLtVFLLL 
                 10.080 
               
               
                 1155 
                 SLPPnVCPVL 
                 10.080 
               
               
                 795 
                 KPGPgACAQL 
                 9.600 
               
               
                 850 
                 KQGLrLLRGL 
                 9.600 
               
               
                 416 
                 RPWArLRVRL 
                 9.600 
               
               
                 175 
                 GQQDdEENEL 
                 9.504 
               
               
                 143 
                 IIPNvGFQEL 
                 9.504 
               
               
                 104 
                 LQVSpIQVEL 
                 9.240 
               
               
                 939 
                 AAPGeVCAKL 
                 9.240 
               
               
                 1800 
                 DYLPtEREVS 
                 9.000 
               
               
                 1495 
                 VYVVkATNLA 
                 9.000 
               
               
                 1596 
                 QYEVdGYNAW 
                 9.000 
               
               
                 958 
                 KQAKaCTSEL 
                 8.800 
               
               
                 429 
                 EGLPaLRLGL 
                 8.640 
               
               
                 1418 
                 RSLKeEFNHF 
                 8.640 
               
               
                 642 
                 ELLPlPENVL 
                 8.640 
               
               
                 183 
                 ELELeLEQDL 
                 8.640 
               
               
                 884 
                 EPQPpLPDVL 
                 8.640 
               
               
                 1609 
                 FWPSqILAGL 
                 8.400 
               
               
                 106 
                 VSPIqVELDL 
                 8.400 
               
               
                 1311 
                 LAEAgISRQL 
                 8.400 
               
               
                 773 
                 NCVRkVAERL 
                 8.400 
               
               
                 635 
                 AMEVeVEELL 
                 8.400 
               
               
                 1937 
                 VYILtGKVEA 
                 8.250 
               
               
                 1494 
                 RVYVvKATNL 
                 8.000 
               
               
                 1872 
                 RNGAgPRCNL 
                 8.000 
               
               
                 1639 
                 KVFLtPPETL 
                 8.000 
               
               
                 422 
                 RVRLyRAEGL 
                 8.000 
               
               
                 479 
                 WNEQlSFVEL 
                 7.920 
               
               
                 1032 
                 QTLSpLWDEL 
                 7.920 
               
               
                 37 
                 CGPEaDIGEL 
                 7.920 
               
               
                 831 
                 NALDrCRGKL 
                 7.920 
               
               
                 1601 
                 GYNAwRDAFW 
                 7.500 
               
               
                 997 
                 LYQArGVLAA 
                 7.500 
               
               
                 1074 
                 GPPVfLGRAL 
                 7.200 
               
               
                 1229 
                 TAPLfHPQDL 
                 7.200 
               
               
                 1539 
                 IFGEiLELSI 
                 7.200 
               
               
                 1047 
                 LIVDgRREHL 
                 7.200 
               
               
                 548 
                 AGLRdSLQGL 
                 7.200 
               
               
                 1137 
                 EVEPqDLAPL 
                 7.200 
               
               
                 1535 
                 QLNPiFGEIL 
                 7.200 
               
               
                 1698 
                 HSPGlLQGSL 
                 7.200 
               
               
                 1302 
                 QGQKsLDPFL 
                 7.200 
               
               
                 1745 
                 DVVLdDENPL 
                 7.200 
               
               
                 571 
                 LLLAvSMQVL 
                 7.200 
               
               
                 1 
                 MALTvSVQRL 
                 7.200 
               
               
                 437 
                 GLLGsLVRAL 
                 7.200 
               
               
                 157 
                 AQLErRAVAL 
                 7.200 
               
               
                 2002 
                 TLVLlLLVLL 
                 7.200 
               
               
                 970 
                 DLLPePSAGL 
                 7.200 
               
               
                 78 
                 GTLViSLQQL 
                 7.200 
               
               
                 2006 
                 LLLVlLTVFL 
                 7.200 
               
               
                 19 
                 RQVKlTFRGF 
                 7.200 
               
               
                 844 
                 SLNLlAKQGL 
                 7.200 
               
               
                 1446 
                 GGEEeGSGHL 
                 7.200 
               
               
                 2025 
                 QISQvIFRPL 
                 6.720 
               
               
                 51 
                 HYGApLAGEC 
                 6.600 
               
               
                 1537 
                 NPIFgEILEL 
                 6.600 
               
               
                 1319 
                 QLLKpPLKKL 
                 6.600 
               
               
                 133 
                 GAPIqDSFEL 
                 6.600 
               
               
                 1482 
                 GIPQnRPIKL 
                 6.600 
               
               
                 1483 
                 IPQNrPIKLL 
                 6.000 
               
               
                 1821 
                 EAEFrQPAVL 
                 6.000 
               
               
                 2007 
                 LLVLlTVFLL 
                 6.000 
               
               
                 1002 
                 GVLAaDDSGL 
                 6.000 
               
               
                 1556 
                 LTVAvFDHDL 
                 6.000 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XVIII 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, B7-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 940 
                 APGEVCAKL 
                 240.000 
               
               
                 134 
                 APIQDSFEL 
                 240.000 
               
               
                 1230 
                 APLFHPQDL 
                 240.000 
               
               
                 774 
                 CVRKVAERL 
                 200.000 
               
               
                 1148 
                 EPHSGRLSL 
                 120.000 
               
               
                 269 
                 CPFYNEYFL 
                 80.000 
               
               
                 107 
                 SPIQVELDL 
                 80.000 
               
               
                 1699 
                 SPGLLQGSL 
                 80.000 
               
               
                 1610 
                 WPSQILAGL 
                 80.000 
               
               
                 431 
                 LPALRLGLL 
                 80.000 
               
               
                 613 
                 TPKAVPQHL 
                 80.000 
               
               
                 454 
                 EPYVRVSFL 
                 80.000 
               
               
                 1483 
                 IPQNRPIKL 
                 80.000 
               
               
                 144 
                 IPNVGFQEL 
                 80.000 
               
               
                 1156 
                 LPPNVCPVL 
                 80.000 
               
               
                 1322 
                 KPPLKKLPL 
                 80.000 
               
               
                 1405 
                 SPKKAVATL 
                 80.000 
               
               
                 492 
                 LTRSLRLQL 
                 60.000 
               
               
                 284 
                 RLRLQDLLL 
                 40.000 
               
               
                 1026 
                 TTRVLEQTL 
                 40.000 
               
               
                 549 
                 GLRDSLQGL 
                 40.000 
               
               
                 282 
                 DTRLRLQDL 
                 40.000 
               
               
                 1963 
                 KGRKQPEPL 
                 40.000 
               
               
                 166 
                 LGRRLARSL 
                 40.000 
               
               
                 632 
                 IPRAMEVEV 
                 40.000 
               
               
                 707 
                 AAVEAQPLL 
                 36.000 
               
               
                 475 
                 AAPEWNEQL 
                 36.000 
               
               
                 333 
                 ATRIGTFRM 
                 30.000 
               
               
                 363 
                 DPRDTRAGT 
                 30.000 
               
               
                 1082 
                 ALAAPRVKL 
                 27.000 
               
               
                 1661 
                 DPEEAQALL 
                 24.000 
               
               
                 1862 
                 GPELCSVQL 
                 24.000 
               
               
                 38 
                 GPEADIGEL 
                 24.000 
               
               
                 889 
                 LPDVLVWML 
                 24.000 
               
               
                 1097 
                 RPELQFFPL 
                 24.000 
               
               
                 1763 
                 YVKSWVKGL 
                 20.000 
               
               
                 1876 
                 GPRCNLFRC 
                 20.000 
               
               
                 867 
                 KVALAKKLL 
                 20.000 
               
               
                 2008 
                 LVLLTVFLL 
                 20.000 
               
               
                 2003 
                 LVLLLLVLL 
                 20.000 
               
               
                 105 
                 QVSPIQVEL 
                 20.000 
               
               
                 1282 
                 DPPEEEGEM 
                 20.000 
               
               
                 1746 
                 VVLDDENPL 
                 20.000 
               
               
                 1557 
                 TVAVFDHDL 
                 20.000 
               
               
                 1224 
                 VVFKDTAPL 
                 20.000 
               
               
                 1313 
                 EAGISRQLL 
                 18.000 
               
               
                 1616 
                 AGLCQRCGL 
                 18.000 
               
               
                 1607 
                 DAFWPSQIL 
                 18.000 
               
               
                 651 
                 LAPCEDFLL 
                 12.000 
               
               
                 800 
                 ACAQLKQAL 
                 12.000 
               
               
                 1841 
                 SANDFLGSL 
                 12.000 
               
               
                 959 
                 QAKACTSEL 
                 12.000 
               
               
                 162 
                 RAVALGRRL 
                 12.000 
               
               
                 870 
                 LAKKLLAKL 
                 12.000 
               
               
                 53 
                 GAPLAGECL 
                 12.000 
               
               
                 936 
                 APGAAPGEV 
                 12.000 
               
               
                 503 
                 DAPLVDAAL 
                 12.000 
               
               
                 706 
                 GAAVEAQPL 
                 12.000 
               
               
                 1116 
                 IAAFQLIEL 
                 12.000 
               
               
                 848 
                 LAKQGLRLL 
                 12.000 
               
               
                 2 
                 ALTVSVQRL 
                 12.000 
               
               
                 686 
                 RAGRLEEQL 
                 12.000 
               
               
                 444 
                 RALHDQRVL 
                 12.000 
               
               
                 825 
                 RTMTRPNAL 
                 12.000 
               
               
                 1718 
                 VPAPPPVDI 
                 12.000 
               
               
                 510 
                 ALATHVPDL 
                 12.000 
               
               
                 1048 
                 IVDGRREHL 
                 9.000 
               
               
                 1873 
                 NGAGPRCNL 
                 9.000 
               
               
                 1504 
                 APADPNGKA 
                 9.000 
               
               
                 1186 
                 EVEQPQVVL 
                 9.000 
               
               
                 905 
                 WARIPAQDV 
                 9.000 
               
               
                 1308 
                 DPFLAEAGI 
                 8.000 
               
               
                 490 
                 PPLTRSLRL 
                 8.000 
               
               
                 676 
                 QPISFEISI 
                 8.000 
               
               
                 670 
                 DPTVASQPI 
                 8.000 
               
               
                 1108 
                 GPWAAGELI 
                 8.000 
               
               
                 1245 
                 QPPLSILVI 
                 8.000 
               
               
                 2022 
                 IPGQISQVI 
                 8.000 
               
               
                 1075 
                 PPVFLGRAL 
                 8.000 
               
               
                 624 
                 SPGAEGPEI 
                 8.000 
               
               
                 1320 
                 LLKPPLKKL 
                 6.000 
               
               
                 1471 
                 AVLFSEPQI 
                 6.000 
               
               
                 643 
                 LLPLPENVL 
                 6.000 
               
               
                 884 
                 EPQPPLPDV 
                 6.000 
               
               
                 996 
                 HLYQARGVL 
                 6.000 
               
               
                 1146 
                 LVEPHSGRL 
                 6.000 
               
               
                 563 
                 QGIWFRGRL 
                 6.000 
               
               
                 430 
                 GLPALRLGL 
                 6.000 
               
               
                 72 
                 FSLRPLGTL 
                 6.000 
               
               
                 542 
                 YGSPPGAGL 
                 6.000 
               
               
                 971 
                 LLPEPSAGL 
                 6.000 
               
               
                 1085 
                 APRVKLMED 
                 6.000 
               
               
                 504 
                 APLVDAALA 
                 6.000 
               
               
                 335 
                 RIGTFRMDL 
                 6.000 
               
               
                 1168 
                 RVEVLFWGL 
                 6.000 
               
               
                 739 
                 DGSGPYFCL 
                 6.000 
               
               
                 379 
                 LSVRARGDL 
                 6.000 
               
               
                 1943 
                 KVEAEFELL 
                 6.000 
               
               
                 1888 
                 RGWWPVVKL 
                 6.000 
               
               
                 886 
                 QPPLPDVLV 
                 6.000 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XIX 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, B7-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1727 
                 KPRQpISYEL 
                 800.000 
               
               
                 1856 
                 MVRGaRGPEL 
                 200.000 
               
               
                 422 
                 RVRLyRAEGL 
                 200.000 
               
               
                 1483 
                 IPQNrPIKLL 
                 120.000 
               
               
                 433 
                 ALRLgLLGSL 
                 120.000 
               
               
                 416 
                 RPWArLRVRL 
                 120.000 
               
               
                 412 
                 VPAErPWARL 
                 120.000 
               
               
                 905 
                 WARIpAQDVL 
                 120.000 
               
               
                 974 
                 EPSAgLPSSL 
                 80.000 
               
               
                 884 
                 EPQPpLPDVL 
                 80.000 
               
               
                 75 
                 RPLGtLVISL 
                 80.000 
               
               
                 1376 
                 DPGDsDGVNL 
                 80.000 
               
               
                 1074 
                 GPPVfLGRAL 
                 80.000 
               
               
                 1134 
                 VPSEvEPQDL 
                 80.000 
               
               
                 1537 
                 NPIFgEILEL 
                 80.000 
               
               
                 320 
                 SPKAfHSQTL 
                 80.000 
               
               
                 489 
                 FPPLtRSLRL 
                 80.000 
               
               
                 206 
                 SPLKsRARAL 
                 80.000 
               
               
                 795 
                 KPGPgACAQL 
                 80.000 
               
               
                 1175 
                 GLRGlGRVHL 
                 60.000 
               
               
                 1633 
                 AVKVgSKVFL 
                 60.000 
               
               
                 1163 
                 VLREfRVEVL 
                 40.000 
               
               
                 282 
                 DTRLrLQDLL 
                 40.000 
               
               
                 1316 
                 ISRQlLKPPL 
                 40.000 
               
               
                 68 
                 CSRVfSLRPL 
                 40.000 
               
               
                 634 
                 RAMEvEVEEL 
                 36.000 
               
               
                 939 
                 AAPGeVCAKL 
                 36.000 
               
               
                 474 
                 AAAPeWNEQL 
                 36.000 
               
               
                 509 
                 AALAtHVPDL 
                 36.000 
               
               
                 382 
                 RARGdLPPPM 
                 30.000 
               
               
                 1639 
                 KVFLtPPETL 
                 30.000 
               
               
                 1081 
                 RALAaPRVKL 
                 27.000 
               
               
                 1685 
                 VPEHvETRPL 
                 24.000 
               
               
                 134 
                 APIQdSFELI 
                 24.000 
               
               
                 1028 
                 RVLEqTLSPL 
                 20.000 
               
               
                 777 
                 KVAErLDQGL 
                 20.000 
               
               
                 649 
                 NVLApCEDFL 
                 20.000 
               
               
                 65 
                 VVNCsRVFSL 
                 20.000 
               
               
                 1223 
                 TVVFkDTAPL 
                 20.000 
               
               
                 2008 
                 LVLLtVFLLL 
                 20.000 
               
               
                 4 
                 TVSVqRLTGL 
                 20.000 
               
               
                 1002 
                 GVLAaDDSGL 
                 20.000 
               
               
                 1745 
                 DVVLdDENPL 
                 20.000 
               
               
                 1494 
                 RVYVvKATNL 
                 20.000 
               
               
                 1170 
                 EVLFwGLRGL 
                 20.000 
               
               
                 747 
                 LPLChCKPCM 
                 20.000 
               
               
                 1455 
                 LVGKfKGSFL 
                 20.000 
               
               
                 232 
                 GVTVlEAQKL 
                 20.000 
               
               
                 943 
                 EVCAkLELFL 
                 20.000 
               
               
                 831 
                 NALDrCRGKL 
                 18.000 
               
               
                 90 
                 AGHLvLREAL 
                 18.000 
               
               
                 1615 
                 LAGLcQRCGL 
                 18.000 
               
               
                 869 
                 ALAKkLLAKL 
                 12.000 
               
               
                 1411 
                 ATLKiYNRSL 
                 12.000 
               
               
                 133 
                 GAPIqDSFEL 
                 12.000 
               
               
                 799 
                 GACAqLKQAL 
                 12.000 
               
               
                 157 
                 AQLErRAVAL 
                 12.000 
               
               
                 591 
                 AQQGsTLSRL 
                 12.000 
               
               
                 165 
                 ALGRrLARSL 
                 12.000 
               
               
                 1241 
                 QPYLqPPLSI 
                 12.000 
               
               
                 706 
                 GAAVeAQPLL 
                 12.000 
               
               
                 1229 
                 TAPLfHPQDL 
                 12.000 
               
               
                 54 
                 APLAgECLSV 
                 12.000 
               
               
                 545 
                 PPGAgLRDSL 
                 12.000 
               
               
                 548 
                 AGLRdSLQGL 
                 12.000 
               
               
                 945 
                 CAKLeLFLRL 
                 12.000 
               
               
                 962 
                 ACTSeLPPDL 
                 12.000 
               
               
                 802 
                 AQLKqALEVL 
                 12.000 
               
               
                 1 
                 MALTvSVQRL 
                 12.000 
               
               
                 1197 
                 AGQGvESEVL 
                 12.000 
               
               
                 442 
                 LVRAlHDQRV 
                 10.000 
               
               
                 568 
                 RGRLlLAVSM 
                 10.000 
               
               
                 936 
                 APGAaPGEVC 
                 9.000 
               
               
                 1872 
                 RNGAgPRCNL 
                 9.000 
               
               
                 588 
                 PPQAqQGSTL 
                 8.000 
               
               
                 1986 
                 NPLKtFVFFI 
                 8.000 
               
               
                 1070 
                 HNKFgPPVFL 
                 6.000 
               
               
                 429 
                 EGLPaLRLGL 
                 6.000 
               
               
                 562 
                 GQGIwFRGRL 
                 6.000 
               
               
                 1859 
                 GARGpELCSV 
                 6.000 
               
               
                 1047 
                 LIVDgRREHL 
                 6.000 
               
               
                 637 
                 EVEVeELLPL 
                 6.000 
               
               
                 963 
                 CTSElPPDLL 
                 6.000 
               
               
                 642 
                 ELLPlPENVL 
                 6.000 
               
               
                 1137 
                 EVEPqDLAPL 
                 6.000 
               
               
                 1319 
                 QLLKpPLKKL 
                 6.000 
               
               
                 970 
                 DLLPePSAGL 
                 6.000 
               
               
                 504 
                 APLVdAALAT 
                 6.000 
               
               
                 988 
                 FSYFqLRAHL 
                 6.000 
               
               
                 378 
                 TLSVrARGDL 
                 6.000 
               
               
                 1085 
                 APRVkLMEDP 
                 6.000 
               
               
                 880 
                 FLAEePQPPL 
                 6.000 
               
               
                 195 
                 EPDVeLSGVM 
                 6.000 
               
               
                 1924 
                 RPEDlEFTDM 
                 6.000 
               
               
                 564 
                 GIWFrGRLLL 
                 6.000 
               
               
                 427 
                 RAEGlPALRL 
                 5.400 
               
               
                 1263 
                 LVGShIVPHM 
                 5.000 
               
               
                 1492 
                 LVRVyVVKAT 
                 5.000 
               
               
                 2001 
                 RTLVlLLLVL 
                 4.000 
               
               
                 1535 
                 QLNPiFGEIL 
                 4.000 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XX 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, B3501-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 9 amino acids and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1282 
                 DPPEEEGEM 
                 120.000 
               
               
                 1976 
                 RPKTSFNWF 
                 120.000 
               
               
                 736 
                 EPMDGSGPY 
                 80.000 
               
               
                 1532 
                 IPKQLNPIF 
                 60.000 
               
               
                 1405 
                 SPKKAVATL 
                 60.000 
               
               
                 613 
                 TPKAVPQHL 
                 60.000 
               
               
                 652 
                 APCEDFLLF 
                 60.000 
               
               
                 1507 
                 DPNGKADPY 
                 40.000 
               
               
                 1322 
                 KPPLKKLPL 
                 40.000 
               
               
                 1973 
                 KPSRPKTSF 
                 40.000 
               
               
                 940 
                 APGEVCAKL 
                 40.000 
               
               
                 134 
                 APIQDSFEL 
                 30.000 
               
               
                 1156 
                 LPPNVCPVL 
                 20.000 
               
               
                 1610 
                 WPSQILAGL 
                 20.000 
               
               
                 454 
                 EPYVRVSFL 
                 20.000 
               
               
                 1230 
                 APLFHPQDL 
                 20.000 
               
               
                 1148 
                 EPHSGRLSL 
                 20.000 
               
               
                 1986 
                 NPLKTFVFF 
                 20.000 
               
               
                 1699 
                 SPGLLQGSL 
                 20.000 
               
               
                 107 
                 SPIQVELDL 
                 20.000 
               
               
                 1269 
                 VPHMLRFTF 
                 20.000 
               
               
                 431 
                 LPALRLGLL 
                 20.000 
               
               
                 269 
                 CPFYNEYFL 
                 20.000 
               
               
                 144 
                 IPNVGFQEL 
                 20.000 
               
               
                 1483 
                 IPQNRPIKL 
                 20.000 
               
               
                 418 
                 WARLRVRLY 
                 18.000 
               
               
                 1594 
                 ASQYEVDGY 
                 15.000 
               
               
                 363 
                 DPRDTRAGT 
                 12.000 
               
               
                 632 
                 IPRAMEVEV 
                 12.000 
               
               
                 1087 
                 RVKLMEDPY 
                 12.000 
               
               
                 624 
                 SPGAEGPEI 
                 12.000 
               
               
                 1408 
                 KAVATLKIY 
                 12.000 
               
               
                 1661 
                 DPEEAQALL 
                 12.000 
               
               
                 1097 
                 RPELQFFPL 
                 12.000 
               
               
                 267 
                 TSCPFYNEY 
                 10.000 
               
               
                 1922 
                 KGRPEDLEF 
                 9.000 
               
               
                 1419 
                 SLKEEFNHF 
                 9.000 
               
               
                 444 
                 RALHDQRVL 
                 9.000 
               
               
                 959 
                 QAKACTSEL 
                 9.000 
               
               
                 38 
                 GPEADIGEL 
                 9.000 
               
               
                 848 
                 LAKQGLRLL 
                 9.000 
               
               
                 870 
                 LAKKLLAKL 
                 9.000 
               
               
                 1466 
                 YPESEAVLF 
                 9.000 
               
               
                 1308 
                 DPFLAEAGI 
                 8.000 
               
               
                 1802 
                 LPTEREVSV 
                 8.000 
               
               
                 1108 
                 GPWAAGELI 
                 8.000 
               
               
                 1245 
                 QPPLSILVI 
                 8.000 
               
               
                 1139 
                 EPQDLAPLV 
                 8.000 
               
               
                 670 
                 DPTVASQPI 
                 8.000 
               
               
                 1718 
                 VPAPPPVDI 
                 8.000 
               
               
                 1487 
                 RPIKLLVRV 
                 8.000 
               
               
                 676 
                 QPISFEISI 
                 8.000 
               
               
                 1550 
                 LPAETELTV 
                 8.000 
               
               
                 2022 
                 IPGQISQVI 
                 8.000 
               
               
                 1207 
                 ASYRESPNF 
                 7.500 
               
               
                 1841 
                 SANDFLGSL 
                 6.000 
               
               
                 889 
                 LPDVLVWML 
                 6.000 
               
               
                 1876 
                 GPRCNLFRC 
                 6.000 
               
               
                 686 
                 RAGRLEEQL 
                 6.000 
               
               
                 333 
                 ATRIGTFRM 
                 6.000 
               
               
                 213 
                 RALAHGDPF 
                 6.000 
               
               
                 1035 
                 SPLWDELLV 
                 6.000 
               
               
                 475 
                 AAPEWNEQL 
                 6.000 
               
               
                 320 
                 SPKAFHSQT 
                 6.000 
               
               
                 1111 
                 AAGELIAAF 
                 6.000 
               
               
                 284 
                 RLRLQDLLL 
                 6.000 
               
               
                 1862 
                 GPELCSVQL 
                 6.000 
               
               
                 1963 
                 KGRKQPEPL 
                 6.000 
               
               
                 526 
                 RAAGFNPTF 
                 6.000 
               
               
                 837 
                 RGKLLVHSL 
                 6.000 
               
               
                 448 
                 DQRVLVEPY 
                 6.000 
               
               
                 162 
                 RAVALGRRL 
                 6.000 
               
               
                 1083 
                 LAAPRVKLM 
                 6.000 
               
               
                 549 
                 GLRDSLQGL 
                 6.000 
               
               
                 707 
                 AAVEAQPLL 
                 6.000 
               
               
                 1265 
                 GSHIVPHML 
                 5.000 
               
               
                 1451 
                 GSGHLVGKF 
                 5.000 
               
               
                 1783 
                 NSLTGEGNF 
                 5.000 
               
               
                 72 
                 FSLRPLGTL 
                 5.000 
               
               
                 200 
                 LSGVMFSPL 
                 5.000 
               
               
                 379 
                 LSVRARGDL 
                 5.000 
               
               
                 1034 
                 LSPLWDELL 
                 5.000 
               
               
                 5 
                 VSVQRLTGL 
                 5.000 
               
               
                 682 
                 ISIGRAGRL 
                 5.000 
               
               
                 2026 
                 ISQVIFRPL 
                 5.000 
               
               
                 1548 
                 ISLPAETEL 
                 5.000 
               
               
                 1095 
                 YQRPELQFF 
                 4.500 
               
               
                 706 
                 GAAVEAQPL 
                 4.500 
               
               
                 651 
                 LAPCEDFLL 
                 4.500 
               
               
                 1344 
                 IPDPEELDW 
                 4.500 
               
               
                 886 
                 QPPLPDVLV 
                 4.000 
               
               
                 1161 
                 CPVLREFRV 
                 4.000 
               
               
                 531 
                 NPTFGPAWV 
                 4.000 
               
               
                 884 
                 EPQPPLPDV 
                 4.000 
               
               
                 936 
                 APGAAPGEV 
                 4.000 
               
               
                 1059 
                 EPPLVIINV 
                 4.000 
               
               
                 1621 
                 RCGLPAPEY 
                 4.000 
               
               
                 109 
                 IQVELDLKY 
                 4.000 
               
               
                 1677 
                 MPGFGIQLV 
                 4.000 
               
               
                 118 
                 QPPEGATGA 
                 4.000 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXI 
               
             
            
               
                   
               
               
                 158P3D2v.17, ORF: 65-6175, Frame +2, B3501-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 29; each 
               
               
                 start position is Specified, the length of the 
               
               
                 peptide is 10 amino acids and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
            
           
           
               
               
               
            
               
                 Pos 
                 Subsequence 
                 Score 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1727 
                 KPRQpISYEL 
                 120.000 
               
               
                 1234 
                 HPQDlPEQPY 
                 80.000 
               
               
                 1487 
                 RPIKlLVRVY 
                 80.000 
               
               
                 1924 
                 RPEDlEFTDM 
                 72.000 
               
               
                 1134 
                 VPSEvEPQDL 
                 60.000 
               
               
                 320 
                 SPKAfHSQTL 
                 60.000 
               
               
                 1376 
                 DPGDsDGVNL 
                 60.000 
               
               
                 75 
                 RPLGtLVISL 
                 40.000 
               
               
                 412 
                 VPAErPWARL 
                 40.000 
               
               
                 747 
                 LPLChCKPCM 
                 40.000 
               
               
                 795 
                 KPGPgACAQL 
                 40.000 
               
               
                 736 
                 EPMDgSGPYF 
                 40.000 
               
               
                 416 
                 RPWArLRVRL 
                 40.000 
               
               
                 382 
                 RARGdLPPPM 
                 36.000 
               
               
                 1035 
                 SPLWdELLVF 
                 30.000 
               
               
                 1802 
                 LPTErEVSVW 
                 30.000 
               
               
                 1815 
                 GPFAlEEAEF 
                 30.000 
               
               
                 1537 
                 NPIFgEILEL 
                 30.000 
               
               
                 269 
                 CPFYnEYFLF 
                 30.000 
               
               
                 1976 
                 RPKTsFNWFV 
                 24.000 
               
               
                 974 
                 EPSAgLPSSL 
                 20.000 
               
               
                 1093 
                 DPYQrPELQF 
                 20.000 
               
               
                 2022 
                 IPGQiSQVIF 
                 20.000 
               
               
                 1059 
                 EPPLvIINVF 
                 20.000 
               
               
                 753 
                 KPCMhVWSCW 
                 20.000 
               
               
                 884 
                 EPQPpLPDVL 
                 20.000 
               
               
                 1483 
                 IPQNrPIKLL 
                 20.000 
               
               
                 1074 
                 GPPVfLGRAL 
                 20.000 
               
               
                 206 
                 SPLKsRARAL 
                 20.000 
               
               
                 118 
                 QPPEgATGAW 
                 20.000 
               
               
                 1847 
                 GSLElQLPDM 
                 20.000 
               
               
                 979 
                 LPSSlHRDDF 
                 20.000 
               
               
                 489 
                 FPPLtRSLRL 
                 20.000 
               
               
                 634 
                 RAMEvEVEEL 
                 18.000 
               
               
                 68 
                 CSRVfSLRPL 
                 15.000 
               
               
                 1316 
                 ISRQlLKPPL 
                 15.000 
               
               
                 886 
                 QPPLpDVLVW 
                 15.000 
               
               
                 981 
                 SSLHrDDFSY 
                 15.000 
               
               
                 1418 
                 RSLKeEFNHF 
                 15.000 
               
               
                 568 
                 RGRLlLAVSM 
                 12.000 
               
               
                 1588 
                 RANCgLASQY 
                 12.000 
               
               
                 195 
                 EPDVeLSGVM 
                 12.000 
               
               
                 1826 
                 QPAVlVLQVW 
                 10.000 
               
               
                 1685 
                 VPEHvETRPL 
                 9.000 
               
               
                 945 
                 CAKLeLFLRL 
                 9.000 
               
               
                 476 
                 APEWnEQLSF 
                 9.000 
               
               
                 905 
                 WARIpAQDVL 
                 9.000 
               
               
                 1593 
                 LASQyEVDGY 
                 9.000 
               
               
                 1986 
                 NPLKtFVFFI 
                 8.000 
               
               
                 1241 
                 QPYLqPPLSI 
                 8.000 
               
               
                 134 
                 APIQdSFELI 
                 8.000 
               
               
                 1725 
                 DIKPrQPISY 
                 6.000 
               
               
                 263 
                 TQRGtSCPFY 
                 6.000 
               
               
                 54 
                 APLAgECLSV 
                 6.000 
               
               
                 1081 
                 RALAaPRVKL 
                 6.000 
               
               
                 1730 
                 QPISyELRVV 
                 6.000 
               
               
                 831 
                 NALDrCRGKL 
                 6.000 
               
               
                 38 
                 GPEAdIGELF 
                 6.000 
               
               
                 1117 
                 AAFQlIELDY 
                 6.000 
               
               
                 1550 
                 LPAEtELTVA 
                 6.000 
               
               
                 322 
                 KAFHsQTLPF 
                 6.000 
               
               
                 332 
                 MATRiGTFRM 
                 6.000 
               
               
                 422 
                 RVRLyRAEGL 
                 6.000 
               
               
                 1297 
                 MPKGpQGQKS 
                 6.000 
               
               
                 1163 
                 VLREfRVEVL 
                 6.000 
               
               
                 1698 
                 HSPGlLQGSL 
                 5.000 
               
               
                 521 
                 ISHPgRAAGF 
                 5.000 
               
               
                 470 
                 VSAEaAAPEW 
                 5.000 
               
               
                 740 
                 GSGPyFCLPL 
                 5.000 
               
               
                 296 
                 VSGVgVTSVL 
                 5.000 
               
               
                 1808 
                 VSVWrRSGPF 
                 5.000 
               
               
                 267 
                 TSCPfYNEYF 
                 5.000 
               
               
                 1758 
                 MSSDiYVKSW 
                 5.000 
               
               
                 1840 
                 ISANdFLGSL 
                 5.000 
               
               
                 1404 
                 VSPKkAVATL 
                 5.000 
               
               
                 1732 
                 ISYElRVVIW 
                 5.000 
               
               
                 483 
                 LSFVeLFPPL 
                 5.000 
               
               
                 106 
                 VSPIqVELDL 
                 5.000 
               
               
                 988 
                 FSYFqLRAHL 
                 5.000 
               
               
                 1979 
                 TSFNwFVNPL 
                 5.000 
               
               
                 1705 
                 GSLHmWIDIF 
                 5.000 
               
               
                 1584 
                 YSHHrANCGL 
                 5.000 
               
               
                 133 
                 GAPIqDSFEL 
                 4.500 
               
               
                 651 
                 LAPCeDFLLF 
                 4.500 
               
               
                 1206 
                 LASYrESPNF 
                 4.500 
               
               
                 1648 
                 LPPGsSSPTV 
                 4.000 
               
               
                 777 
                 KVAErLDQGL 
                 4.000 
               
               
                 1507 
                 DPNGkADPYV 
                 4.000 
               
               
                 1861 
                 RGPElCSVQL 
                 4.000 
               
               
                 1754 
                 LTGEmSSDIY 
                 4.000 
               
               
                 967 
                 LPPDlLPEPS 
                 4.000 
               
               
                 908 
                 IPAQdVLFSV 
                 4.000 
               
               
                 1028 
                 RVLEqTLSPL 
                 4.000 
               
               
                 1973 
                 KPSRpKTSFN 
                 4.000 
               
               
                 887 
                 PPLPdVLVWM 
                 4.000 
               
               
                 240 
                 KLVGvNINPY 
                 4.000 
               
               
                 1643 
                 TPPEtLPPGS 
                 4.000 
               
               
                 398 
                 HCSDiEKNLL 
                 3.000 
               
               
                 1110 
                 WAAGeLIAAF 
                 3.000 
               
               
                 1365 
                 GQHNfDEDEM 
                 3.000 
               
               
                   
               
            
           
         
       
     
     Tables XXII-XLIX 
     
       
         
           
               
             
               
                 TABLE XXII 
               
               
                   
               
               
                 158P3D2v.1-A1-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 8 0 GWWP V VK 
                 1755316491 
               
               
                 188 
                 0 G WWPV V KL 
                 1441796 
               
               
                 314 
                 0 V LLTV F LL 
                 1441795 
               
               
                 62 
                 0 K GLEH D KQ 
                 1441794 
               
               
                 251 
                 0 E LLTV E EA 
                 1441793 
               
               
                 125 
                 0 V LVLQ V WD 
                 1441792 
               
               
                 313 
                 0 0 VLLT V FL 
                 1441792 
               
               
                 250 
                 4 0 ELLT V EE 
                 262154 
               
               
                 312 
                 3 0 0VLL T VF 
                 65803 
               
               
                 230 
                 F T DMGG N VY 
                 36 
               
               
                 34 
                 N T EDVV L DD 
                 25 
               
               
                 47 
                 T G EMSS D IY 
                 25 
               
               
                 18 
                 I K PRQP I SY 
                 21 
               
               
                 126 
                 V L VLQV W DY 
                 20 
               
               
                 71 
                 E T DVHF N SL 
                 19 
               
               
                 51 
                 S S DIYV K SW 
                 18 
               
               
                 97 
                 P T EREV S VW 
                 18 
               
               
                 326 
                 Y T IPGQ I SQ 
                 18 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIII 
               
               
                   
               
               
                 158P3D2v.1-A0201-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWW P VVK 
                 1755316492 
               
               
                 188 
                 0GWWP V VKL 
                 1441812 
               
               
                 314 
                 0VLLT V FLL 
                 1441812 
               
               
                 313 
                 00VLL T VFL 
                 1441807 
               
               
                 251 
                 0ELLT V EEA 
                 1441804 
               
               
                 125 
                 0VLVL Q VWD 
                 1441801 
               
               
                 62 
                 0KGLE H DKQ 
                 1441794 
               
               
                 250 
                 40ELL T VEE 
                 262150 
               
               
                 312 
                 300VL L TVF 
                 65807 
               
               
                 310 
                 LL300 V LLT 
                 29 
               
               
                 309 
                 LLL30 0 VLL 
                 27 
               
               
                 316 
                 LLTVF L LLV 
                 27 
               
               
                 138 
                 SANDF L GSL 
                 25 
               
               
                 232 
                 DMGGN V YIL 
                 24 
               
               
                 307 
                 VLLLL 3 00V 
                 24 
               
               
                 308 
                 LLLL3 0 0VL 
                 24 
               
               
                 315 
                 VLLTV F LLL 
                 24 
               
               
                 320 
                 FLLLV F YTI 
                 24 
               
               
                 327 
                 TIPGQ I SQV 
                 24 
               
               
                 142 
                 FLGSL E LQL 
                 23 
               
               
                 238 
                 YILTG K VEA 
                 22 
               
               
                 31 
                 VIWNT E DVV 
                 20 
               
               
                 145 
                 SLELQ L PDM 
                 20 
               
               
                 149 
                 QLPDM V RGA 
                 20 
               
               
                 157 
                 ARGPE L CSV 
                 20 
               
               
                 236 
                 NVYIL T GKV 
                 20 
               
               
                 248 
                 F240E L LTV 
                 20 
               
               
                 2 
                 WIDIF P QDV 
                 19 
               
               
                 30 
                 VVIWN T EDV 
                 19 
               
               
                 38 
                 VVLDD E NPL 
                 19 
               
               
                 122 
                 A120V L VLQ 
                 19 
               
               
                 239 
                 ILTGK V EAE 
                 19 
               
               
                 283 
                 SFNWF V NPL 
                 19 
               
               
                 287 
                 FVNPL K TFV 
                 19 
               
               
                 290 
                 PLKTF V FFI 
                 19 
               
               
                 311 
                 L300V L LTV 
                 19 
               
               
                 23 
                 PISYE L RVV 
                 18 
               
               
                 118 
                 FRQPA 1 20V 
                 18 
               
               
                 121 
                 PA120 V LVL 
                 18 
               
               
                 302 
                 YWRTL V LLL 
                 18 
               
               
                 335 
                 VIFRP L HK3 
                 18 
               
               
                 39 
                 VLDDE N PLT 
                 17 
               
               
                 96 
                 LPTER E VSV 
                 17 
               
               
                 301 
                 RYWRT L VLL 
                 17 
               
               
                 4 
                 DIFPQ D VPA 
                 16 
               
               
                 10 
                 VPAPP P VDI 
                 16 
               
               
                 24 
                 ISYEL R VVI 
                 16 
               
               
                 46 
                 LTGEM S SDI 
                 16 
               
               
                 64 
                 GLEHD K QET 
                 16 
               
               
                 140 
                 NDFLG S LEL 
                 16 
               
               
                 305 
                 TLVLL L L30 
                 16 
               
               
                 27 
                 ELRVV I WNT 
                 15 
               
               
                 32 
                 IWNTE D VVL 
                 15 
               
               
                 94 
                 DYLPT E REV 
                 15 
               
               
                 126 
                 VLVLQ V WDY 
                 15 
               
               
                 146 
                 LELQL P DMV 
                 15 
               
               
                 166 
                 QLARN G AGP 
                 15 
               
               
                 170 
                 NGAGP R CNL 
                 15 
               
               
                 231 
                 TDMGG N VYI 
                 15 
               
               
                 246 
                 AEF24 0 ELL 
                 15 
               
               
                 253 
                 LLTVE E AEK 
                 15 
               
               
                 298 
                 IWRRY W RTL 
                 15 
               
               
                 321 
                 LLLVF Y TIP 
                 15 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIV 
               
               
                   
               
               
                 158P3D2v.1-A0203-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 124 
                 2 0 VLVL Q VW 
                 1761625411 
               
               
                 249 
                 2 4 0ELL T VE 
                 1761625411 
               
               
                 123 
                 1 2 0VLV L QV 
                 1381322579 
               
               
                 186 
                 1 8 0GWW P VV 
                 1381322579 
               
               
                 62 
                 0 K GLEH D KQ 
                 1346719859 
               
               
                 125 
                 0 V LVLQ V WD 
                 1346719859 
               
               
                 188 
                 0 G WWPV V KL 
                 1346719859 
               
               
                 251 
                 0 E LLTV E EA 
                 1346719859 
               
               
                 313 
                 0 0 VLLT V FL 
                 1346719859 
               
               
                 314 
                 0 V LLTV F LL 
                 1346719859 
               
               
                 312 
                 3 0 0VLL T VF 
                 1315204197 
               
               
                 187 
                 8 0 GWWP V VK 
                 609485069 
               
               
                 250 
                 4 0 ELLT V EE 
                 2911585 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXV 
               
               
                   
               
               
                 158P3D2v.1-A3-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 124 
                 20 V LV LQ VW 
                 1761625417 
               
               
                 249 
                 24 0 EL LT VE 
                 1761625417 
               
               
                 186 
                 18 0 GW WP VV 
                 1381322589 
               
               
                 123 
                 12 0 VL VL QV 
                 1381322585 
               
               
                 125 
                 0V L VL QV WD 
                 1346719876 
               
               
                 314 
                 0V L LT VF LL 
                 1346719872 
               
               
                 313 
                 00 V LL TV FL 
                 1346719864 
               
               
                 188 
                 0G W WP VV KL 
                 1346719863 
               
               
                 251 
                 0E L LT VE EA 
                 1346719862 
               
               
                 62 
                 0K G LE HD KQ 
                 1346719860 
               
               
                 312 
                 30 0 VL LT VF 
                 1315204206 
               
               
                 187 
                 80 G WW PV VK 
                 609485088 
               
               
                 250 
                 40 E LL TV EE 
                 2911590 
               
               
                 334 
                 QV I FR PL HK 
                 31 
               
               
                 183 
                 RL R 18 0G WW 
                 23 
               
               
                 253 
                 LL T VE EA EK 
                 23 
               
               
                 55 
                 YV K SW V6 0K 
                 22 
               
               
                 166 
                 QL A RN GA GP 
                 22 
               
               
                 59 
                 WV 6 0K GL EH 
                 21 
               
               
                 103 
                 SV W RR SG PF 
                 21 
               
               
                 122 
                 A1 2 0V LV LQ 
                 21 
               
               
                 268 
                 RK Q PE PL EK 
                 21 
               
               
                 318 
                 TV F LL LV FY 
                 21 
               
               
                 147 
                 EL Q LP DM VR 
                 20 
               
               
                 273 
                 PL E KP SR PK 
                 20 
               
               
                 161 
                 EL C SV QL AR 
                 19 
               
               
                 195 
                 KL K EA ED VE 
                 19 
               
               
                 206 
                 AQ E AQ AG KK 
                 19 
               
               
                 177 
                 NL F RC RR LR 
                 18 
               
               
                 320 
                 FL L LV FY TI 
                 18 
               
               
                 9 
                 DV P AP PP VD 
                 17 
               
               
                 45 
                 PL T GE MS SD 
                 17 
               
               
                 53 
                 DI Y VK SW V6 
                 17 
               
               
                 73 
                 DV H FN SL TG 
                 17 
               
               
                 126 
                 VL V LQ VW DY 
                 17 
               
               
                 153 
                 MV R GA RG PE 
                 17 
               
               
                 209 
                 AQ A GK KK RK 
                 17 
               
               
                 214 
                 KK R KQ RR RK 
                 17 
               
               
                 258 
                 EA E KR PV GK 
                 17 
               
               
                 300 
                 RR Y WR TL VL 
                 17 
               
               
                 308 
                 LL L L3 00 VL 
                 17 
               
               
                 15 
                 PV D IK PR QP 
                 16 
               
               
                 24 
                 IS Y EL RV VI 
                 16 
               
               
                 29 
                 RV V IW NT ED 
                 16 
               
               
                 78 
                 SL T GE GN FN 
                 16 
               
               
                 142 
                 FL G SL EL QL 
                 16 
               
               
                 193 
                 VV K LK EA ED 
                 16 
               
               
                 201 
                 DV E RE AQ EA 
                 16 
               
               
                 207 
                 QE A QA GK KK 
                 16 
               
               
                 222 
                 KG R PE DL EF 
                 16 
               
               
                 236 
                 NV Y IL TG KV 
                 16 
               
               
                 238 
                 YI L TG KV EA 
                 16 
               
               
                 239 
                 IL T GK VE AE 
                 16 
               
               
                 261 
                 KR P VG KG RK 
                 16 
               
               
                 263 
                 PV G KG RK QP 
                 16 
               
               
                 294 
                 FV F FI WR RY 
                 16 
               
               
                 309 
                 LL L 30 0V LL 
                 16 
               
               
                 315 
                 VL L TV FL LL 
                 16 
               
               
                 327 
                 TI P GQ IS QV 
                 16 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVI 
               
               
                   
               
               
                 158P3D2v.1-A26-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWWPVVK 
                 1755316490 
               
               
                 314 
                 0VLLTVFLL 
                 1441814 
               
               
                 188 
                 0GWWPVVKL 
                 1441805 
               
               
                 313 
                 00VLLTVFL 
                 1441805 
               
               
                 125 
                 0VLVLQVWD 
                 1441803 
               
               
                 251 
                 0ELLTVEEA 
                 1441796 
               
               
                 62 
                 0KGLEHDKQ 
                 1441793 
               
               
                 250 
                 40ELLTVEE 
                 262145 
               
               
                 312 
                 300VLLTVF 
                 65815 
               
               
                 318 
                 TVFLLLVFY 
                 30 
               
               
                 71 
                 ETDVHFNSL 
                 27 
               
               
                 294 
                 FVFFIWRRY 
                 26 
               
               
                 317 
                 LTVFLLLVF 
                 26 
               
               
                 37 
                 DVVLDDENP 
                 22 
               
               
                 73 
                 DVHFNSLTG 
                 21 
               
               
                 101 
                 EVSVWRRSG 
                 21 
               
               
                 135 
                 DRISANDFL 
                 21 
               
               
                 232 
                 DMGGNVYIL 
                 21 
               
               
                 103 
                 SVWRRSGPF 
                 20 
               
               
                 38 
                 VVLDDENPL 
                 19 
               
               
                 230 
                 FTDMGGNVY 
                 19 
               
               
                 245 
                 EAEF240EL 
                 19 
               
               
                 9 
                 DVPAPPPVD 
                 18 
               
               
                 68 
                 DKQETDVHF 
                 18 
               
               
                 126 
                 VLVLQVWDY 
                 18 
               
               
                 201 
                 DVEREAQEA 
                 18 
               
               
                 323 
                 LVFYTIPGQ 
                 18 
               
               
                 4 
                 DIFPQDVPA 
                 17 
               
               
                 200 
                 EDVEREAQE 
                 17 
               
               
                 286 
                 WFVNPLKTF 
                 17 
               
               
                 138 
                 SANDFLGSL 
                 16 
               
               
                 192 
                 PVVKLKEAE 
                 16 
               
               
                 240 
                 LTGKVEAEF 
                 16 
               
               
                 30 
                 VVIWNTEDV 
                 15 
               
               
                 36 
                 EDVVLDDEN 
                 15 
               
               
                 49 
                 EMSSDIYVK 
                 15 
               
               
                 55 
                 YVKSWV60K 
                 15 
               
               
                 254 
                 LTVEEAEKR 
                 15 
               
               
                 304 
                 RTLVLLLL3 
                 15 
               
               
                 306 
                 LVLLLL300 
                 15 
               
               
                 326 
                 YTIPGQISQ 
                 15 
               
               
                 334 
                 QVIFRPLHK 
                 15 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVII 
               
               
                   
               
               
                 158P3D2v.1-B0702-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 249 
                 240ELLTVE 
                 1761625413 
               
               
                 124 
                 20VLVLQVW 
                 1761625412 
               
               
                 186 
                 180GWWPVV 
                 1381322590 
               
               
                 123 
                 120VLVLQV 
                 1381322588 
               
               
                 188 
                 0GWWPVVKL 
                 1346719875 
               
               
                 313 
                 00VLLTVFL 
                 1346719873 
               
               
                 314 
                 0VLLTVFLL 
                 1346719870 
               
               
                 251 
                 0ELLTVEEA 
                 1346719866 
               
               
                 125 
                 0VLVLQVWD 
                 1346719861 
               
               
                 62 
                 0KGLEHDKQ 
                 1346719860 
               
               
                 312 
                 300VLLTVF 
                 1315204205 
               
               
                 187 
                 80GWWPVVK 
                 609485073 
               
               
                 250 
                 40ELLTVEE 
                 2911588 
               
               
                 10 
                 VPAPPPVDI 
                 23 
               
               
                 276 
                 KPSRPKTSF 
                 23 
               
               
                 159 
                 GPELCSVQL 
                 22 
               
               
                 289 
                 NPLKTFVFF 
                 21 
               
               
                 120 
                 QPA120VLV 
                 19 
               
               
                 328 
                 IPGQISQVI 
                 19 
               
               
                 22 
                 QPISYELRV 
                 18 
               
               
                 96 
                 LPTEREVSV 
                 18 
               
               
                 279 
                 RPKTSFNWF 
                 18 
               
               
                 170 
                 NGAGPRCNL 
                 17 
               
               
                 19 
                 KPRQPISYE 
                 16 
               
               
                 191 
                 WPVVKLKEA 
                 16 
               
               
                 266 
                 KGRKQPEPL 
                 15 
               
               
                 300 
                 RRYWRTLVL 
                 15 
               
               
                 302 
                 YWRTLVLLL 
                 15 
               
               
                 32 
                 IWNTEDVVL 
                 14 
               
               
                 119 
                 RQPA120VL 
                 14 
               
               
                 121 
                 PA120VLVL 
                 14 
               
               
                 154 
                 VRGARGPEL 
                 14 
               
               
                 232 
                 DMGGNVYIL 
                 14 
               
               
                 262 
                 RPVGKGRKQ 
                 14 
               
               
                 12 
                 APPPVDIKP 
                 13 
               
               
                 59 
                 WV60KGLEH 
                 13 
               
               
                 71 
                 ETDVHFNSL 
                 13 
               
               
                 105 
                 WRRSGPFAL 
                 13 
               
               
                 142 
                 FLGSLELQL 
                 13 
               
               
                 150 
                 LPDMVRGAR 
                 13 
               
               
                 220 
                 RRKGRPEDL 
                 13 
               
               
                 298 
                 IWRRYWRTL 
                 13 
               
               
                 301 
                 RYWRTLVLL 
                 13 
               
               
                 309 
                 LLL300VLL 
                 13 
               
               
                 315 
                 VLLTVFLLL 
                 13 
               
               
                 332 
                 ISQVIFRPL 
                 13 
               
               
                 6 
                 FPQDVPAPP 
                 12 
               
               
                 16 
                 VDIKPRQPI 
                 12 
               
               
                 57 
                 KSWV60KGL 
                 12 
               
               
                 88 
                 RFVFRFDYL 
                 12 
               
               
                 109 
                 GPFALEEAE 
                 12 
               
               
                 140 
                 NDFLGSLEL 
                 12 
               
               
                 173 
                 GPRCNLFRC 
                 12 
               
               
                 222 
                 KGRPEDLEF 
                 12 
               
               
                 246 
                 AEF240ELL 
                 12 
               
               
                 270 
                 QPEPLEKPS 
                 12 
               
               
                 283 
                 SFNWFVNPL 
                 12 
               
               
                 303 
                 WRTLVLLLL 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVIII 
               
               
                   
               
               
                 158P3D2v.1-B08-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWWPVVK 
                 1755316491 
               
               
                 188 
                 0GWWPVVKL 
                 1441804 
               
               
                 313 
                 00VLLTVFL 
                 1441804 
               
               
                 314 
                 0VLLTVFLL 
                 1441804 
               
               
                 251 
                 0ELLTVEEA 
                 1441795 
               
               
                 125 
                 0VLVLQVWD 
                 1441794 
               
               
                 62 
                 0KGLEHDKQ 
                 1441793 
               
               
                 250 
                 40ELLTVEE 
                 262147 
               
               
                 312 
                 300VLLTVF 
                 65809 
               
               
                 220 
                 RRKGRPEDL 
                 28 
               
               
                 181 
                 CRRLR180G 
                 25 
               
               
                 193 
                 VVKLKEAED 
                 23 
               
               
                 290 
                 PLKTFVFFI 
                 23 
               
               
                 17 
                 DIKPRQPIS 
                 22 
               
               
                 183 
                 RLR180GWW 
                 22 
               
               
                 279 
                 RPKTSFNWF 
                 22 
               
               
                 211 
                 AGKKKRKQR 
                 21 
               
               
                 154 
                 VRGARGPEL 
                 20 
               
               
                 213 
                 KKKRKQRRR 
                 20 
               
               
                 272 
                 EPLEKPSRP 
                 20 
               
               
                 159 
                 GPELCSVQL 
                 19 
               
               
                 171 
                 GAGPRCNLF 
                 19 
               
               
                 191 
                 WPVVKLKEA 
                 19 
               
               
                 212 
                 GKKKRKQRR 
                 19 
               
               
                 239 
                 ILTGKVEAE 
                 19 
               
               
                 264 
                 VGKGRKQPE 
                 19 
               
               
                 88 
                 RFVFRFDYL 
                 18 
               
               
                 120 
                 QPA120VLV 
                 18 
               
               
                 176 
                 CNLFRCRRL 
                 18 
               
               
                 195 
                 KLKEAEDVE 
                 18 
               
               
                 215 
                 KRKQRRRKG 
                 18 
               
               
                 245 
                 EAEF240EL 
                 18 
               
               
                 288 
                 VNPLKTFVF 
                 18 
               
               
                 300 
                 RRYWRTLVL 
                 18 
               
               
                 59 
                 WV60KGLEH 
                 17 
               
               
                 96 
                 LPTEREVSV 
                 17 
               
               
                 105 
                 WRRSGPFAL 
                 17 
               
               
                 142 
                 FLGSLELQL 
                 17 
               
               
                 298 
                 IWRRYWRTL 
                 17 
               
               
                 302 
                 YWRTLVLLL 
                 17 
               
               
                 308 
                 LLLL300VL 
                 17 
               
               
                 309 
                 LLL300VLL 
                 17 
               
               
                 315 
                 VLLTVFLLL 
                 17 
               
               
                 27 
                 ELRVVIWNT 
                 16 
               
               
                 103 
                 SVWRRSGPF 
                 16 
               
               
                 138 
                 SANDFLGSL 
                 16 
               
               
                 218 
                 QRRRKGRPE 
                 16 
               
               
                 262 
                 RPVGKGRKQ 
                 16 
               
               
                 266 
                 KGRKQPEPL 
                 16 
               
               
                 277 
                 PSRPKTSFN 
                 16 
               
               
                 53 
                 DIYVKSWV6 
                 15 
               
               
                 115 
                 EAEFRQPA1 
                 15 
               
               
                 121 
                 PA120VLVL 
                 15 
               
               
                 258 
                 EAEKRPVGK 
                 15 
               
               
                 10 
                 VPAPPPVDI 
                 14 
               
               
                 178 
                 LFRCRRLR1 
                 14 
               
               
                 210 
                 QAGKKKRKQ 
                 14 
               
               
                 217 
                 KQRRRKGRP 
                 14 
               
               
                 257 
                 EEAEKRPVG 
                 14 
               
               
                 320 
                 FLLLVFYTI 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIX 
               
               
                   
               
               
                 158P3D2v.1-B1510-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 249 
                 240ELLTVE 
                 1761625414 
               
               
                 124 
                 20VLVLQVW 
                 1761625413 
               
               
                 186 
                 180GWWPVV 
                 1381322583 
               
               
                 123 
                 120VLVLQV 
                 1381322581 
               
               
                 188 
                 0GWWPVVKL 
                 1346719873 
               
               
                 313 
                 00VLLTVFL 
                 1346719871 
               
               
                 314 
                 0VLLTVFLL 
                 1346719870 
               
               
                 125 
                 0VLVLQVWD 
                 1346719862 
               
               
                 251 
                 0ELLTVEEA 
                 1346719861 
               
               
                 62 
                 0KGLEHDKQ 
                 1346719859 
               
               
                 312 
                 300VLLTVF 
                 1315204206 
               
               
                 187 
                 80GWWPVVK 
                 609485074 
               
               
                 250 
                 40ELLTVEE 
                 2911589 
               
               
                 32 
                 IWNTEDVVL 
                 16 
               
               
                 298 
                 IWRRYWRTL 
                 15 
               
               
                 332 
                 ISQVIFRPL 
                 15 
               
               
                 159 
                 GPELCSVQL 
                 14 
               
               
                 170 
                 NGAGPRCNL 
                 14 
               
               
                 176 
                 CNLFRCRRL 
                 14 
               
               
                 245 
                 EAEF240EL 
                 14 
               
               
                 20 
                 PRQPISYEL 
                 13 
               
               
                 57 
                 KSWV60KGL 
                 13 
               
               
                 105 
                 WRRSGPFAL 
                 13 
               
               
                 119 
                 RQPA120VL 
                 13 
               
               
                 121 
                 PA120VLVL 
                 13 
               
               
                 232 
                 DMGGNVYIL 
                 13 
               
               
                 66 
                 EHDKQETDV 
                 12 
               
               
                 71 
                 ETDVHFNSL 
                 12 
               
               
                 140 
                 NDFLGSLEL 
                 12 
               
               
                 154 
                 VRGARGPEL 
                 12 
               
               
                 220 
                 RRKGRPEDL 
                 12 
               
               
                 266 
                 KGRKQPEPL 
                 12 
               
               
                 300 
                 RRYWRTLVL 
                 12 
               
               
                 301 
                 RYWRTLVLL 
                 12 
               
               
                 302 
                 YWRTLVLLL 
                 12 
               
               
                 308 
                 LLLL300VL 
                 12 
               
               
                 309 
                 LLL300VLL 
                 12 
               
               
                 38 
                 VVLDDENPL 
                 11 
               
               
                 138 
                 SANDFLGSL 
                 11 
               
               
                 283 
                 SFNWFVNPL 
                 11 
               
               
                 74 
                 VHFNSLTGE 
                 10 
               
               
                 83 
                 GNFNWRFVF 
                 10 
               
               
                 88 
                 RFVFRFDYL 
                 10 
               
               
                 135 
                 DRISANDFL 
                 10 
               
               
                 142 
                 FLGSLELQL 
                 10 
               
               
                 246 
                 AEF240ELL 
                 10 
               
               
                 303 
                 WRTLVLLLL 
                 10 
               
               
                 315 
                 VLLTVFLLL 
                 10 
               
               
                 42 
                 DENPLTGEM 
                 9 
               
               
                 68 
                 DKQETDVHF 
                 9 
               
               
                 81 
                 GEGNFNWRF 
                 9 
               
               
                 85 
                 FNWRFVFRF 
                 9 
               
               
                 171 
                 GAGPRCNLF 
                 9 
               
               
                 222 
                 KGRPEDLEF 
                 9 
               
               
                 276 
                 KPSRPKTSF 
                 9 
               
               
                 289 
                 NPLKTFVFF 
                 9 
               
               
                 24 
                 ISYELRVVI 
                 8 
               
               
                 110 
                 PFALEEAEF 
                 8 
               
               
                 145 
                 SLELQLPDM 
                 8 
               
               
                 240 
                 LTGKVEAEF 
                 8 
               
               
                 286 
                 WFVNPLKTF 
                 8 
               
               
                 288 
                 VNPLKTFVF 
                 8 
               
               
                 317 
                 LTVFLLLVF 
                 8 
               
               
                 329 
                 PGQISQVIF 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXX 
               
               
                   
               
               
                 158P3D2v.1-B2705-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 249 
                 240ELLTVE 
                 1761625417 
               
               
                 124 
                 20VLVLQVW 
                 1761625416 
               
               
                 186 
                 180GWWPVV 
                 1381322587 
               
               
                 123 
                 120VLVLQV 
                 1381322583 
               
               
                 188 
                 0GWWPVVKL 
                 1346719874 
               
               
                 313 
                 00VLLTVFL 
                 1346719873 
               
               
                 314 
                 0VLLTVFLL 
                 1346719873 
               
               
                 125 
                 0VLVLQVWD 
                 1346719865 
               
               
                 251 
                 0ELLTVEEA 
                 1346719864 
               
               
                 62 
                 0KGLEHDKQ 
                 1346719863 
               
               
                 312 
                 300VLLTVF 
                 1315204212 
               
               
                 187 
                 80GWWPVVK 
                 609485081 
               
               
                 250 
                 40ELLTVEE 
                 2911589 
               
               
                 300 
                 RRYWRTLVL 
                 28 
               
               
                 261 
                 KRPVGKGRK 
                 27 
               
               
                 220 
                 RRKGRPEDL 
                 26 
               
               
                 20 
                 PRQPISYEL 
                 25 
               
               
                 99 
                 EREVSVWRR 
                 25 
               
               
                 303 
                 WRTLVLLLL 
                 24 
               
               
                 135 
                 DRISANDFL 
                 23 
               
               
                 105 
                 WRRSGPFAL 
                 22 
               
               
                 154 
                 VRGARGPEL 
                 22 
               
               
                 87 
                 WRFVFRFDY 
                 21 
               
               
                 174 
                 PRCNLFRCR 
                 21 
               
               
                 219 
                 RRRKGRPED 
                 20 
               
               
                 140 
                 NDFLGSLEL 
                 19 
               
               
                 185 
                 R180GWWPV 
                 19 
               
               
                 92 
                 RFDYLPTER 
                 18 
               
               
                 106 
                 RRSGPFALE 
                 18 
               
               
                 119 
                 RQPA120VL 
                 18 
               
               
                 182 
                 RRLR180GW 
                 18 
               
               
                 212 
                 GKKKRKQRR 
                 18 
               
               
                 235 
                 GNVYILTGK 
                 18 
               
               
                 268 
                 RKQPEPLEK 
                 18 
               
               
                 81 
                 GEGNFNWRF 
                 17 
               
               
                 83 
                 GNFNWRFVF 
                 17 
               
               
                 159 
                 GPELCSVQL 
                 17 
               
               
                 175 
                 RCNLFRCRR 
                 17 
               
               
                 209 
                 AQAGKKKRK 
                 17 
               
               
                 213 
                 KKKRKQRRR 
                 17 
               
               
                 222 
                 KGRPEDLEF 
                 17 
               
               
                 267 
                 GRKQPEPLE 
                 17 
               
               
                 276 
                 KPSRPKTSF 
                 17 
               
               
                 293 
                 TFVFFIWRR 
                 17 
               
               
                 330 
                 GQISQVIFR 
                 17 
               
               
                 77 
                 NSLTGEGNF 
                 16 
               
               
                 91 
                 FRFDYLPTE 
                 16 
               
               
                 168 
                 ARNGAGPRC 
                 16 
               
               
                 189 
                 GWWPVVKLK 
                 16 
               
               
                 214 
                 KKRKQRRRK 
                 16 
               
               
                 215 
                 KRKQRRRKG 
                 16 
               
               
                 246 
                 AEF240ELL 
                 16 
               
               
                 254 
                 LTVEEAEKR 
                 16 
               
               
                 279 
                 RPKTSFNWF 
                 16 
               
               
                 292 
                 KTFVFFIWR 
                 16 
               
               
                 301 
                 RYWRTLVLL 
                 16 
               
               
                 21 
                 RQPISYELR 
                 15 
               
               
                 32 
                 IWNTEDVVL 
                 15 
               
               
                 49 
                 EMSSDIYVK 
                 15 
               
               
                 88 
                 RFVFRFDYL 
                 15 
               
               
                 111 
                 FALEEAEFR 
                 15 
               
               
                 121 
                 PA120VLVL 
                 15 
               
               
                 147 
                 ELQLPDMVR 
                 15 
               
               
                 157 
                 ARGPELCSV 
                 15 
               
               
                 170 
                 NGAGPRCNL 
                 15 
               
               
                 171 
                 GAGPRCNLF 
                 15 
               
               
                 208 
                 EAQAGKKKR 
                 15 
               
               
                 211 
                 AGKKKRKQR 
                 15 
               
               
                 223 
                 GRPEDLEFT 
                 15 
               
               
                 240 
                 LTGKVEAEF 
                 15 
               
               
                 271 
                 PEPLEKPSR 
                 15 
               
               
                 278 
                 SRPKTSFNW 
                 15 
               
               
                 289 
                 NPLKTFVFF 
                 15 
               
               
                 317 
                 LTVFLLLVF 
                 15 
               
               
                 318 
                 TVFLLLVFY 
                 15 
               
               
                 337 
                 FRPLHK328 
                 15 
               
               
                 28 
                 LRVVIWNTE 
                 14 
               
               
                 98 
                 TEREVSVWR 
                 14 
               
               
                 110 
                 PFALEEAEF 
                 14 
               
               
                 161 
                 ELCSVQLAR 
                 14 
               
               
                 176 
                 CNLFRCRRL 
                 14 
               
               
                 196 
                 LKEAEDVER 
                 14 
               
               
                 205 
                 EAQEAQAGK 
                 14 
               
               
                 206 
                 AQEAQAGKK 
                 14 
               
               
                 207 
                 QEAQAGKKK 
                 14 
               
               
                 216 
                 RKQRRRKGR 
                 14 
               
               
                 232 
                 DMGGNVYIL 
                 14 
               
               
                 266 
                 KGRKQPEPL 
                 14 
               
               
                 273 
                 PLEKPSRPK 
                 14 
               
               
                 286 
                 WFVNPLKTF 
                 14 
               
               
                 294 
                 FVFFIWRRY 
                 14 
               
               
                 308 
                 LLLL300VL 
                 14 
               
               
                 315 
                 VLLTVFLLL 
                 14 
               
               
                 329 
                 PGQISQVIF 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXI 
               
               
                   
               
               
                 158P3D2v.1-B2709-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWWPVVK 
                 1755316491 
               
               
                 314 
                 0VLLTVFLL 
                 1441805 
               
               
                 188 
                 0GWWPVVKL 
                 1441804 
               
               
                 313 
                 00VLLTVFL 
                 1441804 
               
               
                 125 
                 0VLVLQVWD 
                 1441795 
               
               
                 251 
                 0ELLTVEEA 
                 1441795 
               
               
                 62 
                 0KGLEHDKQ 
                 1441792 
               
               
                 250 
                 40ELLTVEE 
                 262145 
               
               
                 312 
                 300VLLTVF 
                 65810 
               
               
                 300 
                 RRYWRTLVL 
                 27 
               
               
                 185 
                 R180GWWPV 
                 24 
               
               
                 220 
                 RRKGRPEDL 
                 24 
               
               
                 20 
                 PRQPISYEL 
                 23 
               
               
                 135 
                 DRISANDFL 
                 22 
               
               
                 303 
                 WRTLVLLLL 
                 22 
               
               
                 105 
                 WRRSGPFAL 
                 21 
               
               
                 154 
                 VRGARGPEL 
                 21 
               
               
                 157 
                 ARGPELCSV 
                 20 
               
               
                 118 
                 FRQPA120V 
                 19 
               
               
                 299 
                 WRRYWRTLV 
                 18 
               
               
                 182 
                 RRLR180GW 
                 16 
               
               
                 88 
                 RFVFRFDYL 
                 15 
               
               
                 159 
                 GPELCSVQL 
                 15 
               
               
                 219 
                 RRRKGRPED 
                 15 
               
               
                 246 
                 AEF240ELL 
                 15 
               
               
                 301 
                 RYWRTLVLL 
                 15 
               
               
                 106 
                 RRSGPFALE 
                 14 
               
               
                 119 
                 RQPA120VL 
                 14 
               
               
                 121 
                 PA120VLVL 
                 14 
               
               
                 223 
                 GRPEDLEFT 
                 14 
               
               
                 267 
                 GRKQPEPLE 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXII 
               
               
                   
               
               
                 158P3D2v.1-B4402-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 124 
                 20VLVLQVW 
                 1761625424 
               
               
                 249 
                 240ELLTVE 
                 1761625414 
               
               
                 123 
                 120VLVLQV 
                 1381322582 
               
               
                 186 
                 180GWWPVV 
                 1381322581 
               
               
                 314 
                 0VLLTVFLL 
                 1346719873 
               
               
                 188 
                 0GWWPVVKL 
                 1346719872 
               
               
                 251 
                 0ELLTVEEA 
                 1346719872 
               
               
                 313 
                 00VLLTVFL 
                 1346719872 
               
               
                 62 
                 0KGLEHDKQ 
                 1346719861 
               
               
                 125 
                 0VLVLQVWD 
                 1346719861 
               
               
                 312 
                 300VLLTVF 
                 1315204209 
               
               
                 187 
                 80GWWPVVK 
                 609485072 
               
               
                 250 
                 40ELLTVEE 
                 2911588 
               
               
                 246 
                 AEF240ELL 
                 28 
               
               
                 81 
                 GEGNFNWRF 
                 21 
               
               
                 116 
                 AEFRQPA12 
                 20 
               
               
                 199 
                 AEDVEREAQ 
                 17 
               
               
                 57 
                 KSWV60KGL 
                 16 
               
               
                 171 
                 GAGPRCNLF 
                 16 
               
               
                 259 
                 AEKRPVGKG 
                 16 
               
               
                 25 
                 SYELRVVIW 
                 15 
               
               
                 51 
                 SSDIYVKSW 
                 15 
               
               
                 71 
                 ETDVHFNSL 
                 15 
               
               
                 83 
                 GNFNWRFVF 
                 15 
               
               
                 140 
                 NDFLGSLEL 
                 15 
               
               
                 222 
                 KGRPEDLEF 
                 15 
               
               
                 274 
                 LEKPSRPKT 
                 15 
               
               
                 286 
                 WFVNPLKTF 
                 15 
               
               
                 315 
                 VLLTVFLLL 
                 15 
               
               
                 318 
                 TVFLLLVFY 
                 15 
               
               
                 18 
                 IKPRQPISY 
                 14 
               
               
                 20 
                 PRQPISYEL 
                 14 
               
               
                 26 
                 YELRVVIWN 
                 14 
               
               
                 42 
                 DENPLTGEM 
                 14 
               
               
                 121 
                 PA120VLVL 
                 14 
               
               
                 245 
                 EAEF240EL 
                 14 
               
               
                 257 
                 EEAEKRPVG 
                 14 
               
               
                 288 
                 VNPLKTFVF 
                 14 
               
               
                 289 
                 NPLKTFVFF 
                 14 
               
               
                 294 
                 FVFFIWRRY 
                 14 
               
               
                 295 
                 VFFIWRRYW 
                 14 
               
               
                 300 
                 RRYWRTLVL 
                 14 
               
               
                 301 
                 RYWRTLVLL 
                 14 
               
               
                 302 
                 YWRTLVLLL 
                 14 
               
               
                 303 
                 WRTLVLLLL 
                 14 
               
               
                 309 
                 LLL300VLL 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIII 
               
               
                   
               
               
                 158P3D2v.1-B5101-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWWPVVK 
                 1755316495 
               
               
                 188 
                 0GWWPVVKL 
                 1441810 
               
               
                 314 
                 0VLLTVFLL 
                 1441803 
               
               
                 313 
                 00VLLTVFL 
                 1441799 
               
               
                 251 
                 0ELLTVEEA 
                 1441797 
               
               
                 125 
                 0VLVLQVWD 
                 1441795 
               
               
                 62 
                 0KGLEHDKQ 
                 1441794 
               
               
                 250 
                 40ELLTVEE 
                 262145 
               
               
                 312 
                 300VLLTVF 
                 65807 
               
               
                 328 
                 IPGQISQVI 
                 25 
               
               
                 121 
                 PA120VLVL 
                 24 
               
               
                 10 
                 VPAPPPVDI 
                 23 
               
               
                 96 
                 LPTEREVSV 
                 23 
               
               
                 24 
                 ISYELRVVI 
                 22 
               
               
                 22 
                 QPISYELRV 
                 21 
               
               
                 120 
                 QPA120VLV 
                 19 
               
               
                 138 
                 SANDFLGSL 
                 18 
               
               
                 320 
                 FLLLVFYTI 
                 18 
               
               
                 159 
                 GPELCSVQL 
                 17 
               
               
                 272 
                 EPLEKPSRP 
                 17 
               
               
                 289 
                 NPLKTFVFF 
                 17 
               
               
                 311 
                 L300VLLTV 
                 17 
               
               
                 324 
                 VFYTIPGQI 
                 17 
               
               
                 12 
                 APPPVDIKP 
                 16 
               
               
                 94 
                 DYLPTEREV 
                 16 
               
               
                 111 
                 FALEEAEFR 
                 16 
               
               
                 245 
                 EAEF240EL 
                 16 
               
               
                 6 
                 FPQDVPAPP 
                 15 
               
               
                 46 
                 LTGEMSSDI 
                 15 
               
               
                 82 
                 EGNFNWRFV 
                 15 
               
               
                 210 
                 QAGKKKRKQ 
                 15 
               
               
                 236 
                 NVYILTGKV 
                 15 
               
               
                 248 
                 F240ELLTV 
                 15 
               
               
                 262 
                 RPVGKGRKQ 
                 15 
               
               
                 31 
                 VIWNTEDVV 
                 14 
               
               
                 44 
                 NPLTGEMSS 
                 14 
               
               
                 150 
                 LPDMVRGAR 
                 14 
               
               
                 170 
                 NGAGPRCNL 
                 14 
               
               
                 198 
                 EAEDVEREA 
                 14 
               
               
                 208 
                 EAQAGKKKR 
                 14 
               
               
                 231 
                 TDMGGNVYI 
                 14 
               
               
                 266 
                 KGRKQPEPL 
                 14 
               
               
                 279 
                 RPKTSFNWF 
                 14 
               
               
                 300 
                 RRYWRTLVL 
                 14 
               
               
                 315 
                 VLLTVFLLL 
                 14 
               
               
                 11 
                 PAPPPVDIK 
                 13 
               
               
                 13 
                 PPPVDIKPR 
                 13 
               
               
                 23 
                 PISYELRVV 
                 13 
               
               
                 32 
                 IWNTEDVVL 
                 13 
               
               
                 129 
                 LQVWDYDRI 
                 13 
               
               
                 146 
                 LELQLPDMV 
                 13 
               
               
                 167 
                 LARNGAGPR 
                 13 
               
               
                 191 
                 WPVVKLKEA 
                 13 
               
               
                 194 
                 VKLKEAEDV 
                 13 
               
               
                 224 
                 RPEDLEFTD 
                 13 
               
               
                 232 
                 DMGGNVYIL 
                 13 
               
               
                 258 
                 EAEKRPVGK 
                 13 
               
               
                 290 
                 PLKTFVFFI 
                 13 
               
               
                 308 
                 LLLL300VL 
                 13 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIV 
               
               
                   
               
               
                 158P3D2v.1-A1-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 124 
                 2 0 VLVL Q VWD 
                 1761625411 
               
               
                 249 
                 2 4 0ELL T VEE 
                 1761625411 
               
               
                 186 
                 1 8 0GWW P VVK 
                 1381322581 
               
               
                 123 
                 1 2 0VLV L QVW 
                 1381322580 
               
               
                 125 
                 0 V LVLQ V WDY 
                 1346719875 
               
               
                 314 
                 0 V LLTV F LLL 
                 1346719870 
               
               
                 188 
                 0 G WWPV V KLK 
                 1346719862 
               
               
                 313 
                 0 0 VLLT V FLL 
                 1346719862 
               
               
                 62 
                 0 K GLEH D KQE 
                 1346719859 
               
               
                 251 
                 0 E LLTV E EAE 
                 1346719859 
               
               
                 312 
                 3 0 0VLL T VFL 
                 1315204197 
               
               
                 187 
                 8 0 GWWP V VKL 
                 609485072 
               
               
                 250 
                 4 0 ELLT V EEA 
                 2911596 
               
               
                 17 
                 D I KPRQ P ISY 
                 23 
               
               
                 46 
                 L T GEMS S DIY 
                 21 
               
               
                 317 
                 L T VFLL L VFY 
                 21 
               
               
                 71 
                 E T DVHF N SLT 
                 19 
               
               
                 39 
                 V L DDEN P LTG 
                 18 
               
               
                 230 
                 F T DMGG N VYI 
                 18 
               
               
                 25 
                 S Y ELRV V IWN 
                 17 
               
               
                 139 
                 A N DFLG S LEL 
                 17 
               
               
                 229 
                 E F TDMG G NVY 
                 17 
               
               
                 34 
                 N T EDVV L DDE 
                 16 
               
               
                 51 
                 S S DIYV K SWV 
                 16 
               
               
                 86 
                 N W RFVF R FDY 
                 16 
               
               
                 97 
                 P T EREV S VWR 
                 16 
               
               
                 112 
                 A L EEAE F RQP 
                 15 
               
               
                 293 
                 T F VFFI W RRY 
                 15 
               
               
                 47 
                 T G EMSS D IYV 
                 14 
               
               
                 145 
                 S L ELQL P DMV 
                 14 
               
               
                 206 
                 A Q EAQA G KKK 
                 14 
               
               
                 270 
                 Q P EPLE K PSR 
                 14 
               
               
                 273 
                 P L EKPS R PKT 
                 14 
               
               
                 159 
                 G P ELCS V QLA 
                 13 
               
               
                 224 
                 R P EDLE F TDM 
                 13 
               
               
                 256 
                 V E EAEK R PVG 
                 13 
               
               
                 258 
                 E A EKRP V GKG 
                 13 
               
               
                 80 
                 T G EGNF N WRF 
                 12 
               
               
                 115 
                 E A EFRQ P A12 
                 12 
               
               
                 189 
                 G W WPVV K LKE 
                 12 
               
               
                 245 
                 E A EF24 0 ELL 
                 12 
               
               
                 304 
                 R T LVLL L L30 
                 12 
               
               
                 326 
                 Y T IPGQ I SQV 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXV 
               
               
                   
               
               
                 158P3D2v.1-A0201-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWW P VVKL 
                 1755316508 
               
               
                 314 
                 0VLLT V FLLL 
                 1441809 
               
               
                 313 
                 00VLL T VFLL 
                 1441806 
               
               
                 125 
                 0VLVL Q VWDY 
                 1441802 
               
               
                 188 
                 0GWWP V VKLK 
                 1441796 
               
               
                 251 
                 0ELLT V EEAE 
                 1441795 
               
               
                 62 
                 0KGLE H DKQE 
                 1441791 
               
               
                 250 
                 40ELL T VEEA 
                 262153 
               
               
                 312 
                 300VL L TVFL 
                 65814 
               
               
                 310 
                 LL300 V LLTV 
                 37 
               
               
                 122 
                 A120V L VLQV 
                 28 
               
               
                 315 
                 VLLTV F LLLV 
                 28 
               
               
                 95 
                 YLPTE R EVSV 
                 26 
               
               
                 308 
                 LLLL3 0 0VLL 
                 26 
               
               
                 247 
                 EF240 E LLTV 
                 25 
               
               
                 309 
                 LLL30 0 VLLT 
                 24 
               
               
                 326 
                 YTIPG Q ISQV 
                 24 
               
               
                 156 
                 GARGP E LCSV 
                 23 
               
               
                 307 
                 VLLLL 3 00VL 
                 23 
               
               
                 31 
                 VIWNT E DVVL 
                 22 
               
               
                 297 
                 FIWRR Y WRTL 
                 22 
               
               
                 145 
                 SLELQ L PDMV 
                 21 
               
               
                 137 
                 ISAND F LGSL 
                 20 
               
               
                 128 
                 VLQVW D YDRI 
                 19 
               
               
                 153 
                 MVRGA R GPEL 
                 19 
               
               
                 231 
                 TDMGG N VYIL 
                 18 
               
               
                 306 
                 LVLLL L 300V 
                 18 
               
               
                 327 
                 TIPGQ I SQVI 
                 18 
               
               
                 331 
                 QISQV I FRPL 
                 18 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVI 
               
               
                   
               
               
                 158P3D2v.1-A0203-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 124 
                 2 0 VLVL Q VWD 
                 1761625411 
               
               
                 249 
                 2 4 0ELL T VEE 
                 1761625411 
               
               
                 123 
                 1 2 0VLV L QVW 
                 1381322579 
               
               
                 186 
                 1 8 0GWW P VVK 
                 1381322579 
               
               
                 251 
                 0 E LLTV E EAE 
                 1346719868 
               
               
                 62 
                 0 K GLEH D KQE 
                 1346719859 
               
               
                 125 
                 0 V LVLQ V WDY 
                 1346719859 
               
               
                 188 
                 0 G WWPV V KLK 
                 1346719859 
               
               
                 313 
                 0 0 VLLT V FLL 
                 1346719859 
               
               
                 314 
                 0 V LLTV F LLL 
                 1346719859 
               
               
                 312 
                 3 0 0VLL T VFL 
                 1315204197 
               
               
                 187 
                 8 0 GWWP V VKL 
                 609485069 
               
               
                 250 
                 4 0 ELLT V EEA 
                 2911595 
               
               
                 202 
                 V E REAQ E AQA 
                 18 
               
               
                 3 
                 I D IFPQ D VPA 
                 10 
               
               
                 103 
                 S V WRRS G PFA 
                 10 
               
               
                 107 
                 R S GPFA L EEA 
                 10 
               
               
                 113 
                 L E EAEF R QPA 
                 10 
               
               
                 130 
                 Q V WDYD R ISA 
                 10 
               
               
                 148 
                 L Q LPDM V RGA 
                 10 
               
               
                 159 
                 G P ELCS V QLA 
                 10 
               
               
                 163 
                 C S VQLA R NGA 
                 10 
               
               
                 190 
                 W W PVVK L KEA 
                 10 
               
               
                 197 
                 K E AEDV E REA 
                 10 
               
               
                 200 
                 E D VERE A QEA 
                 10 
               
               
                 237 
                 V Y ILTG K VEA 
                 10 
               
               
                 4 
                 D I FPQD V PAP 
                 9 
               
               
                 104 
                 V W RRSG P FAL 
                 9 
               
               
                 108 
                 S G PFAL E EAE 
                 9 
               
               
                 114 
                 E E AEFR Q PA1 
                 9 
               
               
                 131 
                 V W DYDR I SAN 
                 9 
               
               
                 149 
                 Q L PDMV R GAR 
                 9 
               
               
                 160 
                 P E LCSV Q LAR 
                 9 
               
               
                 164 
                 S V QLAR N GAG 
                 9 
               
               
                 191 
                 W P VVKL K EAE 
                 9 
               
               
                 198 
                 E A EDVE R EAQ 
                 9 
               
               
                 201 
                 D V EREA Q EAQ 
                 9 
               
               
                 203 
                 E R EAQE A QAG 
                 9 
               
               
                 238 
                 Y I LTGK V EAE 
                 9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVII 
               
               
                   
               
               
                 158P3D2v.1-A3-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 124 
                 20 V LV LQ VWD 
                 1761625417 
               
               
                 249 
                 24 0 EL LT VEE 
                 1761625416 
               
               
                 186 
                 18 0 GW WP VVK 
                 1381322604 
               
               
                 123 
                 12 0 VL VL QVW 
                 1381322583 
               
               
                 125 
                 0V L VL QV WDY 
                 1346719877 
               
               
                 314 
                 0V L LT VF LLL 
                 1346719875 
               
               
                 188 
                 0G W WP VV KLK 
                 1346719869 
               
               
                 62 
                 0K G LE HD KQE 
                 1346719863 
               
               
                 251 
                 0E L LT VE EAE 
                 1346719863 
               
               
                 313 
                 00 V LL TV FLL 
                 1346719860 
               
               
                 312 
                 30 0 VL LT VFL 
                 1315204201 
               
               
                 187 
                 80 G WW PV VKL 
                 609485073 
               
               
                 250 
                 40 E LL TV EEA 
                 2911586 
               
               
                 183 
                 RL R 18 0G WWP 
                 25 
               
               
                 252 
                 EL L TV EE AEK 
                 25 
               
               
                 166 
                 QL A RN GA GPR 
                 24 
               
               
                 195 
                 KL K EA ED VER 
                 23 
               
               
                 122 
                 A1 2 0V LV LQV 
                 22 
               
               
                 236 
                 NV Y IL TG KVE 
                 22 
               
               
                 17 
                 DI K PR QP ISY 
                 21 
               
               
                 239 
                 IL T GK VE AEF 
                 21 
               
               
                 287 
                 FV N PL KT FVF 
                 21 
               
               
                 316 
                 LL T VF LL LVF 
                 21 
               
               
                 149 
                 QL P DM VR GAR 
                 20 
               
               
                 39 
                 VL D DE NP LTG 
                 19 
               
               
                 153 
                 MV R GA RG PEL 
                 18 
               
               
                 204 
                 RE A QE AQ AGK 
                 18 
               
               
                 206 
                 AQ E AQ AG KKK 
                 18 
               
               
                 213 
                 KK K RK QR RRK 
                 18 
               
               
                 9 
                 DV P AP PP VDI 
                 17 
               
               
                 23 
                 PI S YE LR VVI 
                 17 
               
               
                 27 
                 EL R VV IW NTE 
                 17 
               
               
                 30 
                 VV I WN TE DVV 
                 17 
               
               
                 31 
                 VI W NT ED VVL 
                 17 
               
               
                 103 
                 SV W RR SG PFA 
                 17 
               
               
                 112 
                 AL E EA EF RQP 
                 17 
               
               
                 136 
                 RI S AN DF LGS 
                 17 
               
               
                 177 
                 NL F RC RR LR1 
                 17 
               
               
                 247 
                 EF 2 40 EL LTV 
                 17 
               
               
                 253 
                 LL T VE EA EKR 
                 17 
               
               
                 257 
                 EE A EK RP VGK 
                 17 
               
               
                 260 
                 EK R PV GK GRK 
                 17 
               
               
                 307 
                 VL L LL 30 0VL 
                 17 
               
               
                 308 
                 LL L L3 00 VLL 
                 17 
               
               
                 310 
                 LL 3 00 VL LTV 
                 17 
               
               
                 53 
                 DI Y VK SW V60 
                 16 
               
               
                 89 
                 FV F RF DY LPT 
                 16 
               
               
                 95 
                 YL P TE RE VSV 
                 16 
               
               
                 127 
                 LV L QV WD YDR 
                 16 
               
               
                 130 
                 QV W DY DR ISA 
                 16 
               
               
                 333 
                 SQ V IF RP LHK 
                 16 
               
               
                 334 
                 QV I FR PL HK3 
                 16 
               
               
                 29 
                 RV V IW NT EDV 
                 15 
               
               
                 45 
                 PL T GE MS SDI 
                 15 
               
               
                 147 
                 EL Q LP DM VRG 
                 15 
               
               
                 193 
                 VV K LK EA EDV 
                 15 
               
               
                 205 
                 EA Q EA QA GKK 
                 15 
               
               
                 243 
                 KV E AE F2 40E 
                 15 
               
               
                 267 
                 GR K QP EP LEK 
                 15 
               
               
                 297 
                 FI W RR YW RTL 
                 15 
               
               
                 309 
                 LL L 30 0V LLT 
                 15 
               
               
                 315 
                 VL L TV FL LLV 
                 15 
               
               
                 323 
                 LV F YT IP GQI 
                 15 
               
               
                 10 
                 VP A PP PV DIK 
                 14 
               
               
                 48 
                 GE M SS DI YVK 
                 14 
               
               
                 64 
                 GL E HD KQ ETD 
                 14 
               
               
                 101 
                 EV S VW RR SGP 
                 14 
               
               
                 145 
                 SL E LQ LP DMV 
                 14 
               
               
                 164 
                 SV Q LA RN GAG 
                 14 
               
               
                 201 
                 DV E RE AQ EAQ 
                 14 
               
               
                 221 
                 RK G RP ED LEF 
                 14 
               
               
                 229 
                 EF T DM GG NVY 
                 14 
               
               
                 259 
                 AE K RP VG KGR 
                 14 
               
               
                 320 
                 FL L LV FY TIP 
                 14 
               
               
                 327 
                 TI P GQ IS QVI 
                 14 
               
               
                 2 
                 WI D IF PQ DVP 
                 13 
               
               
                 38 
                 VV L DD EN PLT 
                 13 
               
               
                 54 
                 IY V KS WV 60K 
                 13 
               
               
                 78 
                 SL T GE GN FNW 
                 13 
               
               
                 157 
                 AR G PE LC SVQ 
                 13 
               
               
                 161 
                 EL C SV QL ARN 
                 13 
               
               
                 192 
                 PV V KL KE AED 
                 13 
               
               
                 272 
                 EP L EK PS RPK 
                 13 
               
               
                 306 
                 LV L LL L3 00V 
                 13 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVIII 
               
               
                   
               
               
                 158P3D2v.1-A26-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWWPVVKL 
                 1755316503 
               
               
                 125 
                 0VLVLQVWDY 
                 1441816 
               
               
                 314 
                 0VLLTVFLLL 
                 1441811 
               
               
                 313 
                 00VLLTVFLL 
                 1441808 
               
               
                 188 
                 0GWWPVVKLK 
                 1441796 
               
               
                 62 
                 0KGLEHDKQE 
                 1441793 
               
               
                 251 
                 0ELLTVEEAE 
                 1441793 
               
               
                 250 
                 40ELLTVEEA 
                 262148 
               
               
                 312 
                 300VLLTVFL 
                 65810 
               
               
                 37 
                 DVVLDDENPL 
                 30 
               
               
                 317 
                 LTVFLLLVFY 
                 26 
               
               
                 17 
                 DIKPRQPISY 
                 25 
               
               
                 229 
                 EFTDMGGNVY 
                 23 
               
               
                 4 
                 DIFPQDVPAP 
                 22 
               
               
                 73 
                 DVHFNSLTGE 
                 22 
               
               
                 101 
                 EVSVWRRSGP 
                 21 
               
               
                 46 
                 LTGEMSSDIY 
                 20 
               
               
                 275 
                 EKPSRPKTSF 
                 20 
               
               
                 334 
                 QVIFRPLHK3 
                 20 
               
               
                 71 
                 ETDVHFNSLT 
                 19 
               
               
                 153 
                 MVRGARGPEL 
                 19 
               
               
                 201 
                 DVEREAQEAQ 
                 19 
               
               
                 287 
                 FVNPLKTFVF 
                 19 
               
               
                 318 
                 TVFLLLVFYT 
                 19 
               
               
                 326 
                 YTIPGQISQV 
                 19 
               
               
                 9 
                 DVPAPPPVDI 
                 18 
               
               
                 82 
                 EGNFNWRFVF 
                 18 
               
               
                 141 
                 DFLGSLELQL 
                 18 
               
               
                 245 
                 EAEF240ELL 
                 18 
               
               
                 282 
                 TSFNWFVNPL 
                 17 
               
               
                 285 
                 NWFVNPLKTF 
                 17 
               
               
                 133 
                 DYDRISANDF 
                 16 
               
               
                 200 
                 EDVEREAQEA 
                 16 
               
               
                 247 
                 EF240ELLTV 
                 16 
               
               
                 258 
                 EAEKRPVGKG 
                 16 
               
               
                 292 
                 KTFVFFIWRR 
                 16 
               
               
                 323 
                 LVFYTIPGQI 
                 16 
               
               
                 89 
                 FVFRFDYLPT 
                 15 
               
               
                 161 
                 ELCSVQLARN 
                 15 
               
               
                 293 
                 TFVFFIWRRY 
                 15 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIX 
               
               
                   
               
               
                 158P3D2v.1-B0702-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 10 amino acids, and the 
               
               
                 end position for each peptide is the start 
               
               
                 position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 249 
                 240ELLTVEE 
                 1761625414 
               
               
                 124 
                 20VLVLQVWD 
                 1761625413 
               
               
                 186 
                 180GWWPVVK 
                 1381322585 
               
               
                 123 
                 120VLVLQVW 
                 1381322580 
               
               
                 314 
                 0VLLTVFLLL 
                 1346719872 
               
               
                 313 
                 00VLLTVFLL 
                 1346719870 
               
               
                 62 
                 0KGLEHDKQE 
                 1346719860 
               
               
                 125 
                 0VLVLQVWDY 
                 1346719860 
               
               
                 188 
                 0GWWPVVKLK 
                 1346719860 
               
               
                 251 
                 0ELLTVEEAE 
                 1346719860 
               
               
                 312 
                 300VLLTVFL 
                 1315204211 
               
               
                 187 
                 80GWWPVVKL 
                 609485083 
               
               
                 250 
                 40ELLTVEEA 
                 2911592 
               
               
                 120 
                 QPA120VLVL 
                 25 
               
               
                 19 
                 KPRQPISYEL 
                 23 
               
               
                 328 
                 IPGQISQVIF 
                 20 
               
               
                 224 
                 RPEDLEFTDM 
                 19 
               
               
                 289 
                 NPLKTFVFFI 
                 19 
               
               
                 109 
                 GPFALEEAEF 
                 18 
               
               
                 279 
                 RPKTSFNWFV 
                 18 
               
               
                 22 
                 QPISYELRVV 
                 17 
               
               
                 159 
                 GPELCSVQLA 
                 17 
               
               
                 299 
                 WRRYWRTLVL 
                 16 
               
               
                 139 
                 ANDFLGSLEL 
                 15 
               
               
                 153 
                 MVRGARGPEL 
                 15 
               
               
                 276 
                 KPSRPKTSFN 
                 15 
               
               
                 12 
                 APPPVDIKPR 
                 14 
               
               
                 141 
                 DFLGSLELQL 
                 14 
               
               
                 169 
                 RNGAGPRCNL 
                 14 
               
               
                 219 
                 RRRKGRPEDL 
                 14 
               
               
                 231 
                 TDMGGNVYIL 
                 14 
               
               
                 262 
                 RPVGKGRKQP 
                 14 
               
               
                 301 
                 RYWRTLVLLL 
                 14 
               
               
                 302 
                 YWRTLVLLLL 
                 14 
               
               
                 331 
                 QISQVIFRPL 
                 14 
               
               
                 10 
                 VPAPPPVDIK 
                 13 
               
               
                 31 
                 VIWNTEDVVL 
                 13 
               
               
                 104 
                 VWRRSGPFAL 
                 13 
               
               
                 134 
                 YDRISANDFL 
                 13 
               
               
                 158 
                 RGPELCSVQL 
                 13 
               
               
                 173 
                 GPRCNLFRCR 
                 13 
               
               
                 300 
                 RRYWRTLVLL 
                 13 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XL 
               
               
                   
               
               
                 158P3D2v.1-B08-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLI 
               
               
                   
               
               
                 158P3D2v.1-B1510-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLII 
               
               
                   
               
               
                 158P3D2v.1-B2705-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIII 
               
               
                   
               
               
                 158P3D2v.1-B2709-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIV 
               
               
                   
               
               
                 158P3D2v.1-B4402-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 10 amino acids, and the 
               
               
                 end position for each peptide is the start 
               
               
                 position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWWPVVKL 
                 1755316503 
               
               
                 314 
                 0VLLTVFLLL 
                 1441807 
               
               
                 251 
                 0ELLTVEEAE 
                 1441806 
               
               
                 313 
                 00VLLTVFLL 
                 1441805 
               
               
                 125 
                 0VLVLQVWDY 
                 1441804 
               
               
                 62 
                 0KGLEHDKQE 
                 1441796 
               
               
                 188 
                 0GWWPVVKLK 
                 1441796 
               
               
                 250 
                 40ELLTVEEA 
                 262146 
               
               
                 312 
                 300VLLTVFL 
                 65814 
               
               
                 70 
                 QETDVHFNSL 
                 23 
               
               
                 244 
                 VEAEF240EL 
                 20 
               
               
                 246 
                 AEF240ELLT 
                 19 
               
               
                 116 
                 AEFRQPA120 
                 18 
               
               
                 274 
                 LEKPSRPKTS 
                 17 
               
               
                 285 
                 NWFVNPLKTF 
                 17 
               
               
                 259 
                 AEKRPVGKGR 
                 16 
               
               
                 17 
                 DIKPRQPISY 
                 15 
               
               
                 26 
                 YELRVVIWNT 
                 15 
               
               
                 48 
                 GEMSSDIYVK 
                 15 
               
               
                 50 
                 MSSDIYVKSW 
                 15 
               
               
                 114 
                 EEAEFRQPA1 
                 15 
               
               
                 139 
                 ANDFLGSLEL 
                 15 
               
               
                 199 
                 AEDVEREAQE 
                 15 
               
               
                 229 
                 EFTDMGGNVY 
                 15 
               
               
                 282 
                 TSFNWFVNPL 
                 15 
               
               
                 287 
                 FVNPLKTFVF 
                 15 
               
               
                 82 
                 EGNFNWRFVF 
                 14 
               
               
                 104 
                 VWRRSGPFAL 
                 14 
               
               
                 118 
                 FRQPA120VL 
                 14 
               
               
                 120 
                 QPA120VLVL 
                 14 
               
               
                 133 
                 DYDRISANDF 
                 14 
               
               
                 141 
                 DFLGSLELQL 
                 14 
               
               
                 160 
                 PELCSVQLAR 
                 14 
               
               
                 170 
                 NGAGPRCNLF 
                 14 
               
               
                 245 
                 EAEF240ELL 
                 14 
               
               
                 257 
                 EEAEKRPVGK 
                 14 
               
               
                 278 
                 SRPKTSFNWF 
                 14 
               
               
                 288 
                 VNPLKTFVFF 
                 14 
               
               
                 294 
                 FVFFIWRRYW 
                 14 
               
               
                 301 
                 RYWRTLVLLL 
                 14 
               
               
                 302 
                 YWRTLVLLLL 
                 14 
               
               
                 308 
                 LLLL300VLL 
                 14 
               
               
                 31 
                 VIWNTEDVVL 
                 13 
               
               
                 42 
                 DENPLTGEMS 
                 13 
               
               
                 56 
                 VKSWV60KGL 
                 13 
               
               
                 87 
                 WRFVFRFDYL 
                 13 
               
               
                 158 
                 RGPELCSVQL 
                 13 
               
               
                 207 
                 QEAQAGKKKR 
                 13 
               
               
                 225 
                 PEDLEFTDMG 
                 13 
               
               
                 228 
                 LEFTDMGGNV 
                 13 
               
               
                 231 
                 TDMGGNVYIL 
                 13 
               
               
                 271 
                 PEPLEKPSRP 
                 13 
               
               
                 275 
                 EKPSRPKTSF 
                 13 
               
               
                 277 
                 PSRPKTSFNW 
                 13 
               
               
                 297 
                 FIWRRYWRTL 
                 13 
               
               
                 299 
                 WRRYWRTLVL 
                 13 
               
               
                 300 
                 RRYWRTLVLL 
                 13 
               
               
                 307 
                 VLLLL300VL 
                 13 
               
               
                 316 
                 LLTVFLLLVF 
                 13 
               
               
                 331 
                 QISQVIFRPL 
                 13 
               
               
                 12 
                 APPPVDIKPR 
                 12 
               
               
                 23 
                 PISYELRVVI 
                 12 
               
               
                 24 
                 ISYELRVVIW 
                 12 
               
               
                 37 
                 DVVLDDENPL 
                 12 
               
               
                 76 
                 FNSLTGEGNF 
                 12 
               
               
                 78 
                 SLTGEGNFNW 
                 12 
               
               
                 81 
                 GEGNFNWRFV 
                 12 
               
               
                 84 
                 NFNWRFVFRF 
                 12 
               
               
                 86 
                 NWRFVFRFDY 
                 12 
               
               
                 96 
                 LPTEREVSVW 
                 12 
               
               
                 100 
                 REVSVWRRSG 
                 12 
               
               
                 109 
                 GPFALEEAEF 
                 12 
               
               
                 137 
                 ISANDFLGSL 
                 12 
               
               
                 146 
                 LELQLPDMVR 
                 12 
               
               
                 175 
                 RCNLFRCRRL 
                 12 
               
               
                 182 
                 RRLR180GWW 
                 12 
               
               
                 197 
                 KEAEDVEREA 
                 12 
               
               
                 221 
                 RKGRPEDLEF 
                 12 
               
               
                 256 
                 VEEAEKRPVG 
                 12 
               
               
                 265 
                 GKGRKQPEPL 
                 12 
               
               
                 290 
                 PLKTFVFFIW 
                 12 
               
               
                 311 
                 L300VLLTVF 
                 12 
               
               
                 317 
                 LTVFLLLVFY 
                 12 
               
               
                 319 
                 VFLLLVFYTI 
                 12 
               
               
                 323 
                 LVFYTIPGQI 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLV 
               
               
                   
               
               
                 158P3D2v.1-B5101-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVI 
               
               
                   
               
               
                 158P3D2v.1-DRB1 0101-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is the start 
               
               
                 position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 124 
                 20VLVLQVWDYDRIS 
                 1761625427 
               
               
                 249 
                 240ELLTVEEAEKRP 
                 1761625412 
               
               
                 123 
                 120VLVLQVWDYDRI 
                 1381322595 
               
               
                 186 
                 180GWWPVVKLKEAE 
                 1381322580 
               
               
                 188 
                 0GWWPVVKLKEAEDV 
                 1346719884 
               
               
                 313 
                 00VLLTVFLLLVFYT 
                 1346719881 
               
               
                 251 
                 0ELLTVEEAEKRPVG 
                 1346719878 
               
               
                 314 
                 0VLLTVFLLLVFYTI 
                 1346719876 
               
               
                 125 
                 0VLVLQVWDYDRISA 
                 1346719873 
               
               
                 62 
                 0KGLEHDKQETDVHF 
                 1346719867 
               
               
                 312 
                 300VLLTVFLLLVFY 
                 1315204214 
               
               
                 187 
                 80GWWPVVKLKEAED 
                 609485086 
               
               
                 250 
                 40ELLTVEEAEKRPV 
                 2911600 
               
               
                 300 
                 RRYWRTLVLLLL300 
                 36 
               
               
                 131 
                 VWDYDRISANDFLGS 
                 32 
               
               
                 322 
                 LLVFYTIPGQISQVI 
                 32 
               
               
                 285 
                 NWFVNPLKTFVFFIW 
                 31 
               
               
                 305 
                 TLVLLLL300VLLTV 
                 31 
               
               
                 73 
                 DVHFNSLTGEGNFNW 
                 29 
               
               
                 143 
                 LGSLELQLPDMVRGA 
                 29 
               
               
                 234 
                 GGNVYILTGKVEAEF 
                 28 
               
               
                 245 
                 EAEF240ELLTVEEA 
                 28 
               
               
                 325 
                 FYTIPGQISQVIFRP 
                 28 
               
               
                 102 
                 VSVWRRSGPFALEEA 
                 27 
               
               
                 181 
                 CRRLR180GWWPVVK 
                 27 
               
               
                 191 
                 WPVVKLKEAEDVERE 
                 27 
               
               
                 317 
                 LTVFLLLVFYTIPGQ 
                 27 
               
               
                 27 
                 ELRVVIWNTEDVVLD 
                 26 
               
               
                 151 
                 PDMVRGARGPELCSV 
                 26 
               
               
                 227 
                 DLEFTDMGGNVYILT 
                 26 
               
               
                 40 
                 LDDENPLTGEMSSDI 
                 25 
               
               
                 140 
                 NDFLGSLELQLPDMV 
                 25 
               
               
                 237 
                 VYILTGKVEAEF240 
                 25 
               
               
                 304 
                 RTLVLLLL300VLLT 
                 25 
               
               
                 3 
                 IDIFPQDVPAPPPVD 
                 24 
               
               
                 90 
                 VFRFDYLPTEREVSV 
                 24 
               
               
                 101 
                 EVSVWRRSGPFALEE 
                 24 
               
               
                 137 
                 ISANDFLGSLELQLP 
                 24 
               
               
                 308 
                 LLLL300VLLTVFLL 
                 24 
               
               
                 318 
                 TVFLLLVFYTIPGQI 
                 24 
               
               
                 43 
                 ENPLTGEMSSDIYVK 
                 23 
               
               
                 329 
                 PGQISQVIFRPLHK3 
                 23 
               
               
                 2 
                 WIDIFPQDVPAPPPV 
                 22 
               
               
                 4 
                 DIFPQDVPAPPPVDI 
                 22 
               
               
                 7 
                 PQDVPAPPPVDIKPR 
                 22 
               
               
                 12 
                 APPPVDIKPRQPISY 
                 22 
               
               
                 120 
                 QPA120VLVLQVWDY 
                 22 
               
               
                 156 
                 GARGPELCSVQLARN 
                 22 
               
               
                 233 
                 MGGNVYILTGKVEAE 
                 22 
               
               
                 310 
                 LL300VLLTVFLLLV 
                 22 
               
               
                 321 
                 LLLVFYTIPGQISQV 
                 22 
               
               
                 296 
                 FFIWRRYWRTLVLLL 
                 21 
               
               
                 86 
                 NWRFVFRFDYLPTER 
                 20 
               
               
                 108 
                 SGPFALEEAEFRQPA 
                 20 
               
               
                 115 
                 EAEFRQPA120VLVL 
                 20 
               
               
                 149 
                 QLPDMVRGARGPELC 
                 20 
               
               
                 235 
                 GNVYILTGKVEAEF2 
                 20 
               
               
                 284 
                 FNWFVNPLKTFVFFI 
                 20 
               
               
                 13 
                 PPPVDIKPRQPISYE 
                 19 
               
               
                 49 
                 EMSSDIYVKSWV60K 
                 19 
               
               
                 52 
                 SDIYVKSWV60KGLE 
                 19 
               
               
                 56 
                 VKSWV60KGLEHDKQ 
                 19 
               
               
                 82 
                 EGNFNWRFVFRFDYL 
                 19 
               
               
                 84 
                 NFNWRFVFRFDYLPT 
                 19 
               
               
                 92 
                 RFDYLPTEREVSVWR 
                 19 
               
               
                 161 
                 ELCSVQLARNGAGPR 
                 19 
               
               
                 190 
                 WWPVVKLKEAEDVER 
                 19 
               
               
                 283 
                 SFNWFVNPLKTFVFF 
                 19 
               
               
                 35 
                 TEDVVLDDENPLTGE 
                 18 
               
               
                 99 
                 EREVSVWRRSGPFAL 
                 18 
               
               
                 110 
                 PFALEEAEFRQPA12 
                 18 
               
               
                 117 
                 EFRQPA120VLVLQV 
                 18 
               
               
                 134 
                 YDRISANDFLGSLEL 
                 18 
               
               
                 139 
                 ANDFLGSLELQLPDM 
                 18 
               
               
                 163 
                 CSVQLARNGAGPRCN 
                 18 
               
               
                 198 
                 EAEDVEREAQEAQAG 
                 18 
               
               
                 217 
                 KQRRRKGRPEDLEFT 
                 18 
               
               
                 222 
                 KGRPEDLEFTDMGGN 
                 18 
               
               
                 253 
                 LLTVEEAEKRPVGKG 
                 18 
               
               
                 299 
                 WRRYWRTLVLLLL30 
                 18 
               
               
                 303 
                 WRTLVLLLL300VLL 
                 18 
               
               
                 306 
                 LVLLLL300VLLTVF 
                 18 
               
               
                 311 
                 L300VLLTVFLLLVF 
                 18 
               
               
                 331 
                 QISQVIFRPLHK328 
                 18 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVII 
               
               
                   
               
               
                 158P3D2v.1-DRB1 0301-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is the start 
               
               
                 position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWWPVVKLKEAED 
                 1755316499 
               
               
                 62 
                 0KGLEHDKQETDVHF 
                 1441819 
               
               
                 314 
                 0VLLTVFLLLVFYTI 
                 1441813 
               
               
                 313 
                 00VLLTVFLLLVFYT 
                 1441806 
               
               
                 251 
                 0ELLTVEEAEKRPVG 
                 1441805 
               
               
                 125 
                 0VLVLQVWDYDRISA 
                 1441804 
               
               
                 188 
                 0GWWPVVKLKEAEDV 
                 1441793 
               
               
                 250 
                 40ELLTVEEAEKRPV 
                 262164 
               
               
                 312 
                 300VLLTVFLLLVFY 
                 65825 
               
               
                 35 
                 TEDVVLDDENPLTGE 
                 37 
               
               
                 237 
                 VYILTGKVEAEF240 
                 34 
               
               
                 36 
                 EDVVLDDENPLTGEM 
                 30 
               
               
                 139 
                 ANDFLGSLELQLPDM 
                 26 
               
               
                 306 
                 LVLLLL300VLLTVF 
                 24 
               
               
                 47 
                 TGEMSSDIYVKSWV6 
                 23 
               
               
                 305 
                 TLVLLLL300VLLTV 
                 21 
               
               
                 308 
                 LLLL300VLLTVFLL 
                 21 
               
               
                 15 
                 PVDIKPRQPISYELR 
                 20 
               
               
                 29 
                 RVVIWNTEDVVLDDE 
                 20 
               
               
                 245 
                 EAEF240ELLTVEEA 
                 20 
               
               
                 285 
                 NWFVNPLKTFVFFIW 
                 20 
               
               
                 3 
                 IDIFPQDVPAPPPVD 
                 19 
               
               
                 76 
                 FNSLTGEGNFNWRFV 
                 19 
               
               
                 88 
                 RFVFRFDYLPTEREV 
                 19 
               
               
                 151 
                 PDMVRGARGPELCSV 
                 19 
               
               
                 190 
                 WWPVVKLKEAEDVER 
                 19 
               
               
                 227 
                 DLEFTDMGGNVYILT 
                 19 
               
               
                 13 
                 PPPVDIKPRQPISYE 
                 18 
               
               
                 21 
                 RQPISYELRVVIWNT 
                 18 
               
               
                 135 
                 DRISANDFLGSLELQ 
                 18 
               
               
                 162 
                 LCSVQLARNGAGPRC 
                 18 
               
               
                 175 
                 RCNLFRCRRLR180G 
                 18 
               
               
                 221 
                 RKGRPEDLEFTDMGG 
                 18 
               
               
                 253 
                 LLTVEEAEKRPVGKG 
                 18 
               
               
                 271 
                 PEPLEKPSRPKTSFN 
                 18 
               
               
                 295 
                 VFFIWRRYWRTLVLL 
                 18 
               
               
                 329 
                 PGQISQVIFRPLHK3 
                 18 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVIII 
               
               
                   
               
               
                 158P3D2v.1-DRB1 0401-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is the start 
               
               
                 position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 124 
                 20VLVLQVWDYDRIS 
                 1761625419 
               
               
                 249 
                 240ELLTVEEAEKRP 
                 1761625411 
               
               
                 123 
                 120VLVLQVWDYDRI 
                 1381322593 
               
               
                 186 
                 180GWWPVVKLKEAE 
                 1381322585 
               
               
                 188 
                 0GWWPVVKLKEAEDV 
                 1346719881 
               
               
                 62 
                 0KGLEHDKQETDVHF 
                 1346719879 
               
               
                 313 
                 00VLLTVFLLLVFYT 
                 1346719879 
               
               
                 125 
                 0VLVLQVWDYDRISA 
                 1346719873 
               
               
                 251 
                 0ELLTVEEAEKRPVG 
                 1346719873 
               
               
                 314 
                 0VLLTVFLLLVFYTI 
                 1346719873 
               
               
                 312 
                 300VLLTVFLLLVFY 
                 1315204211 
               
               
                 187 
                 80GWWPVVKLKEAED 
                 609485085 
               
               
                 250 
                 40ELLTVEEAEKRPV 
                 2911605 
               
               
                 281 
                 KTSFNWFVNPLKTFV 
                 28 
               
               
                 300 
                 RRYWRTLVLLLL300 
                 28 
               
               
                 21 
                 RQPISYELRVVIWNT 
                 26 
               
               
                 36 
                 EDVVLDDENPLTGEM 
                 26 
               
               
                 43 
                 ENPLTGEMSSDIYVK 
                 26 
               
               
                 199 
                 AEDVEREAQEAQAGK 
                 26 
               
               
                 111 
                 FALEEAEFRQPA120 
                 24 
               
               
                 245 
                 EAEF240ELLTVEEA 
                 24 
               
               
                 73 
                 DVHFNSLTGEGNFNW 
                 22 
               
               
                 84 
                 NFNWRFVFRFDYLPT 
                 22 
               
               
                 90 
                 VFRFDYLPTEREVSV 
                 22 
               
               
                 92 
                 RFDYLPTEREVSVWR 
                 22 
               
               
                 129 
                 LQVWDYDRISANDFL 
                 22 
               
               
                 296 
                 FFIWRRYWRTLVLLL 
                 22 
               
               
                 308 
                 LLLL300VLLTVFLL 
                 22 
               
               
                 317 
                 LTVFLLLVFYTIPGQ 
                 22 
               
               
                 322 
                 LLVFYTIPGQISQVI 
                 22 
               
               
                 323 
                 LVFYTIPGQISQVIF 
                 22 
               
               
                 27 
                 ELRVVIWNTEDVVLD 
                 20 
               
               
                 35 
                 TEDVVLDDENPLTGE 
                 20 
               
               
                 47 
                 TGEMSSDIYVKSWV6 
                 20 
               
               
                 76 
                 FNSLTGEGNFNWRFV 
                 20 
               
               
                 87 
                 WRFVFRFDYLPTERE 
                 20 
               
               
                 93 
                 FDYLPTEREVSVWRR 
                 20 
               
               
                 120 
                 QPA120VLVLQVWDY 
                 20 
               
               
                 128 
                 VLQVWDYDRISANDF 
                 20 
               
               
                 151 
                 PDMVRGARGPELCSV 
                 20 
               
               
                 159 
                 GPELCSVQLARNGAG 
                 20 
               
               
                 162 
                 LCSVQLARNGAGPRC 
                 20 
               
               
                 241 
                 TGKVEAEF240ELLT 
                 20 
               
               
                 253 
                 LLTVEEAEKRPVGKG 
                 20 
               
               
                 271 
                 PEPLEKPSRPKTSFN 
                 20 
               
               
                 285 
                 NWFVNPLKTFVFFIW 
                 20 
               
               
                 292 
                 KTFVFFIWRRYWRTL 
                 20 
               
               
                 304 
                 RTLVLLLL300VLLT 
                 20 
               
               
                 305 
                 TLVLLLL300VLLTV 
                 20 
               
               
                 316 
                 LLTVFLLLVFYTIPG 
                 20 
               
               
                 319 
                 VFLLLVFYTIPGQIS 
                 20 
               
               
                 325 
                 FYTIPGQISQVIFRP 
                 20 
               
               
                 329 
                 PGQISQVIFRPLHK3 
                 20 
               
               
                 59 
                 WV60KGLEHDKQETD 
                 18 
               
               
                 67 
                 HDKQETDVHFNSLTG 
                 18 
               
               
                 112 
                 ALEEAEFRQPA120V 
                 18 
               
               
                 137 
                 ISANDFLGSLELQLP 
                 18 
               
               
                 156 
                 GARGPELCSVQLARN 
                 18 
               
               
                 161 
                 ELCSVQLARNGAGPR 
                 18 
               
               
                 196 
                 LKEAEDVEREAQEAQ 
                 18 
               
               
                 202 
                 VEREAQEAQAGKKKR 
                 18 
               
               
                 233 
                 MGGNVYILTGKVEAE 
                 18 
               
               
                 242 
                 GKVEAEF240ELLTV 
                 18 
               
               
                 310 
                 LL300VLLTVFLLLV 
                 18 
               
               
                 331 
                 QISQVIFRPLHK328 
                 18 
               
               
                 3 
                 IDIFPQDVPAPPPVD 
                 16 
               
               
                 30 
                 VVIWNTEDVVLDDEN 
                 16 
               
               
                 52 
                 SDIYVKSWV60KGLE 
                 16 
               
               
                 88 
                 RFVFRFDYLPTEREV 
                 16 
               
               
                 102 
                 VSVWRRSGPFALEEA 
                 16 
               
               
                 108 
                 SGPFALEEAEFRQPA 
                 16 
               
               
                 115 
                 EAEFRQPA120VLVL 
                 16 
               
               
                 131 
                 VWDYDRISANDFLGS 
                 16 
               
               
                 139 
                 ANDFLGSLELQLPDM 
                 16 
               
               
                 235 
                 GNVYILTGKVEAEF2 
                 16 
               
               
                 284 
                 FNWFVNPLKTFVFFI 
                 16 
               
               
                 291 
                 LKTFVFFIWRRYWRT 
                 16 
               
               
                 293 
                 TFVFFIWRRYWRTLV 
                 16 
               
               
                 299 
                 WRRYWRTLVLLLL30 
                 16 
               
               
                 13 
                 PPPVDIKPRQPISYE 
                 15 
               
               
                 7 
                 PQDVPAPPPVDIKPR 
                 14 
               
               
                 25 
                 SYELRVVIWNTEDVV 
                 14 
               
               
                 28 
                 LRVVIWNTEDVVLDD 
                 14 
               
               
                 29 
                 RVVIWNTEDVVLDDE 
                 14 
               
               
                 37 
                 DVVLDDENPLTGEMS 
                 14 
               
               
                 99 
                 EREVSVWRRSGPFAL 
                 14 
               
               
                 110 
                 PFALEEAEFRQPA12 
                 14 
               
               
                 126 
                 VLVLQVWDYDRISAN 
                 14 
               
               
                 134 
                 YDRISANDFLGSLEL 
                 14 
               
               
                 140 
                 NDFLGSLELQLPDMV 
                 14 
               
               
                 143 
                 LGSLELQLPDMVRGA 
                 14 
               
               
                 147 
                 ELQLPDMVRGARGPE 
                 14 
               
               
                 150 
                 LPDMVRGARGPELCS 
                 14 
               
               
                 175 
                 RCNLFRCRRLR180G 
                 14 
               
               
                 190 
                 WWPVVKLKEAEDVER 
                 14 
               
               
                 193 
                 VVKLKEAEDVEREAQ 
                 14 
               
               
                 225 
                 PEDLEFTDMGGNVYI 
                 14 
               
               
                 230 
                 FTDMGGNVYILTGKV 
                 14 
               
               
                 234 
                 GGNVYILTGKVEAEF 
                 14 
               
               
                 288 
                 VNPLKTFVFFIWRRY 
                 14 
               
               
                 303 
                 WRTLVLLLL300VLL 
                 14 
               
               
                 306 
                 LVLLLL300VLLTVF 
                 14 
               
               
                 307 
                 VLLLL300VLLTVFL 
                 14 
               
               
                 318 
                 TVFLLLVFYTIPGQI 
                 14 
               
               
                 320 
                 FLLLVFYTIPGQISQ 
                 14 
               
               
                 321 
                 LLLVFYTIPGQISQV 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIX 
               
               
                   
               
               
                 158P3D2v.1-DRB1 1101-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 3; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is the start 
               
               
                 position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 187 
                 80GWWPVVKLKEAED 
                 1755316517 
               
               
                 188 
                 0GWWPVVKLKEAEDV 
                 1441815 
               
               
                 313 
                 00VLLTVFLLLVFYT 
                 1441805 
               
               
                 125 
                 0VLVLQVWDYDRISA 
                 1441804 
               
               
                 251 
                 0ELLTVEEAEKRPVG 
                 1441802 
               
               
                 314 
                 0VLLTVFLLLVFYTI 
                 1441802 
               
               
                 62 
                 0KGLEHDKQETDVHF 
                 1441798 
               
               
                 250 
                 40ELLTVEEAEKRPV 
                 262156 
               
               
                 312 
                 300VLLTVFLLLVFY 
                 65811 
               
               
                 92 
                 RFDYLPTEREVSVWR 
                 25 
               
               
                 84 
                 NFNWRFVFRFDYLPT 
                 24 
               
               
                 235 
                 GNVYILTGKVEAEF2 
                 24 
               
               
                 322 
                 LLVFYTIPGQISQVI 
                 23 
               
               
                 147 
                 ELQLPDMVRGARGPE 
                 22 
               
               
                 292 
                 KTFVFFIWRRYWRTL 
                 22 
               
               
                 98 
                 TEREVSVWRRSGPFA 
                 21 
               
               
                 99 
                 EREVSVWRRSGPFAL 
                 21 
               
               
                 128 
                 VLQVWDYDRISANDF 
                 20 
               
               
                 161 
                 ELCSVQLARNGAGPR 
                 20 
               
               
                 178 
                 LFRCRRLR180GWWP 
                 20 
               
               
                 140 
                 NDFLGSLELQLPDMV 
                 19 
               
               
                 227 
                 DLEFTDMGGNVYILT 
                 19 
               
               
                 293 
                 TFVFFIWRRYWRTLV 
                 19 
               
               
                 296 
                 FFIWRRYWRTLVLLL 
                 19 
               
               
                 300 
                 RRYWRTLVLLLL300 
                 19 
               
               
                 305 
                 TLVLLLL300VLLTV 
                 19 
               
               
                 318 
                 TVFLLLVFYTIPGQI 
                 19 
               
               
                 3 
                 IDIFPQDVPAPPPVD 
                 18 
               
               
                 90 
                 VFRFDYLPTEREVSV 
                 18 
               
               
                 175 
                 RCNLFRCRRLR180G 
                 18 
               
               
                 284 
                 FNWFVNPLKTFVFFI 
                 18 
               
               
                 317 
                 LTVFLLLVFYTIPGQ 
                 18 
               
               
                 86 
                 NWRFVFRFDYLPTER 
                 17 
               
               
                 177 
                 NLFRCRRLR180GWW 
                 17 
               
               
                 49 
                 EMSSDIYVKSWV60K 
                 16 
               
               
                 67 
                 HDKQETDVHFNSLTG 
                 16 
               
               
                 73 
                 DVHFNSLTGEGNFNW 
                 16 
               
               
                 131 
                 VWDYDRISANDFLGS 
                 16 
               
               
                 172 
                 AGPRCNLFRCRRLR1 
                 16 
               
               
                 212 
                 GKKKRKQRRRKGRPE 
                 16 
               
               
                 214 
                 KKRKQRRRKGRPEDL 
                 16 
               
               
                 245 
                 EAEF240ELLTVEEA 
                 16 
               
               
                 258 
                 EAEKRPVGKGRKQPE 
                 16 
               
               
                 330 
                 GQISQVIFRPLHK32 
                 16 
               
               
                 21 
                 RQPISYELRVVIWNT 
                 15 
               
               
                 53 
                 DIYVKSWV60KGLEH 
                 15 
               
               
                 59 
                 WV60KGLEHDKQETD 
                 15 
               
               
                 253 
                 LLTVEEAEKRPVGKG 
                 15 
               
               
                 254 
                 LTVEEAEKRPVGKGR 
                 15 
               
               
                 271 
                 PEPLEKPSRPKTSFN 
                 15 
               
               
                 13 
                 PPPVDIKPRQPISYE 
                 14 
               
               
                 111 
                 FALEEAEFRQPA120 
                 14 
               
               
                 145 
                 SLELQLPDMVRGARG 
                 14 
               
               
                 148 
                 LQLPDMVRGARGPEL 
                 14 
               
               
                 150 
                 LPDMVRGARGPELCS 
                 14 
               
               
                 159 
                 GPELCSVQLARNGAG 
                 14 
               
               
                 196 
                 LKEAEDVEREAQEAQ 
                 14 
               
               
                 260 
                 EKRPVGKGRKQPEPL 
                 14 
               
               
                 261 
                 KRPVGKGRKQPEPLE 
                 14 
               
               
                 268 
                 RKQPEPLEKPSRPKT 
                 14 
               
               
                 308 
                 LLLL300VLLTVFLL 
                 14 
               
               
                 2 
                 WIDIFPQDVPAPPPV 
                 13 
               
               
                 12 
                 APPPVDIKPRQPISY 
                 13 
               
               
                 25 
                 SYELRVVIWNTEDVV 
                 13 
               
               
                 34 
                 NTEDVVLDDENPLTG 
                 13 
               
               
                 47 
                 TGEMSSDIYVKSWV6 
                 13 
               
               
                 110 
                 PFALEEAEFRQPA12 
                 13 
               
               
                 176 
                 CNLFRCRRLR180GW 
                 13 
               
               
                 234 
                 GGNVYILTGKVEAEF 
                 13 
               
               
                 281 
                 KTSFNWFVNPLKTFV 
                 13 
               
               
                 285 
                 NWFVNPLKTFVFFIW 
                 13 
               
               
                 291 
                 LKTFVFFIWRRYWRT 
                 13 
               
               
                 316 
                 LLTVFLLLVFYTIPG 
                 13 
               
               
                 319 
                 VFLLLVFYTIPGQIS 
                 13 
               
               
                 329 
                 PGQISQVIFRPLHK3 
                 13 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXII 
               
               
                   
               
               
                 158P3D2v.18, ORF: 2932-4764, Frame +1, 
               
               
                 610 aa, Part A, A1-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length of 
               
               
                 the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 62 
                 P Q PGCP P AY 
                 16 
               
               
                 22 
                 R H PGQP L VR 
                 10 
               
               
                 46 
                 K P RPAP L SC 
                 10 
               
               
                 70 
                 Y T GTVL E QT 
                 10 
               
               
                 44 
                 A S KPRP A PL 
                 9 
               
               
                 69 
                 A Y TGTV L EQ 
                 9 
               
               
                 21 
                 R R HPGQ P LV 
                 8 
               
               
                 30 
                 R S VPSW S SS 
                 8 
               
               
                 36 
                 S S SCGW A WA 
                 8 
               
               
                 52 
                 L S CPRI C CP 
                 8 
               
               
                 61 
                 S P QPGC P PA 
                 8 
               
               
                 72 
                 G T VLEQ T LS 
                 8 
               
               
                 73 
                 T V LEQT L SP 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 
               
               
                 610 aa, Part A, A0201-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 24 
                 PGQPL V RSV 
                 16 
               
               
                 21 
                 RRHPG Q PLV 
                 15 
               
               
                 44 
                 ASKPR P APL 
                 15 
               
               
                 66 
                 CPPAY T GTV 
                 15 
               
               
                 27 
                 PLVRS V PSW 
                 14 
               
               
                 70 
                 YTGTV L EQT 
                 14 
               
               
                 20 
                 LRRHP G QPL 
                 13 
               
               
                 73 
                 TVLEQ T LSP 
                 13 
               
               
                 12 
                 SWQNC A FWL 
                 12 
               
               
                 71 
                 TGTVL E QTL 
                 12 
               
               
                 61 
                 SPQPG C PPA 
                 11 
               
               
                 19 
                 WLRRH P GQP 
                 10 
               
               
                 36 
                 SSSCG W AWA 
                 10 
               
               
                 49 
                 PAPLS C PRI 
                 10 
               
               
                 56 
                 RICCP S PQP 
                 10 
               
               
                 69 
                 AYTGT V LEQ 
                 10 
               
               
                 42 
                 AWASK P RPA 
                 9 
               
               
                 31 
                 SVPSW S SSC 
                 8 
               
               
                 51 
                 PLSCP R ICC 
                 8 
               
               
                 52 
                 LSCPR I CCP 
                 8 
               
               
                 65 
                 GCPPA Y TGT 
                 8 
               
               
                 67 
                 PPAYT G TVL 
                 8 
               
               
                 68 
                 PAYTG T VLE 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part A, A0203-9-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXV 
               
               
                   
               
               
                 158P3D2 v.18, ORF: 2932-4764, Frame +1, 
               
               
                 610 aa, Part A, A3-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 19 
                 WL R RH PG QP 
                 21 
               
               
                 28 
                 LV R SV PS WS 
                 19 
               
               
                 22 
                 RH P GQ PL VR 
                 18 
               
               
                 73 
                 TV L EQ TL SP 
                 18 
               
               
                 31 
                 SV P SW SS SC 
                 16 
               
               
                 38 
                 SC G WA WA SK 
                 16 
               
               
                 27 
                 PL V RS VP SW 
                 15 
               
               
                 46 
                 KP R PA PL SC 
                 15 
               
               
                 56 
                 RI C CP SP QP 
                 15 
               
               
                 44 
                 AS K PR PA PL 
                 13 
               
               
                 30 
                 RS V PS WS SS 
                 12 
               
               
                 48 
                 RP A PL SC PR 
                 12 
               
               
                 14 
                 QN C AF WL RR 
                 11 
               
               
                 51 
                 PL S CP RI CC 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 
               
               
                 Part A, 610 aa, A26, 9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 73 
                 TVLEQTLSP 
                 15 
               
               
                 31 
                 SVPSWSSSC 
                 14 
               
               
                 70 
                 YTGTVLEQT 
                 13 
               
               
                 28 
                 LVRSVPSWS 
                 12 
               
               
                 71 
                 TGTVLEQTL 
                 12 
               
               
                 72 
                 GTVLEQTLS 
                 12 
               
               
                 62 
                 PQPGCPPAY 
                 11 
               
               
                 10 
                 RSSWQNCAF 
                 9 
               
               
                 12 
                 SWQNCAFWL 
                 9 
               
               
                 20 
                 LRRHPGQPL 
                 9 
               
               
                 27 
                 PLVRSVPSW 
                 9 
               
               
                 44 
                 ASKPRPAPL 
                 9 
               
               
                 67 
                 PPAYTGTVL 
                 9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1 
               
               
                 610 aa, Part A, B0702-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 67 
                 PPAYTGTVL 
                 24 
               
               
                 63 
                 QPGCPPAYT 
                 21 
               
               
                 61 
                 SPQPGCPPA 
                 19 
               
               
                 66 
                 CPPAYTGTV 
                 18 
               
               
                 20 
                 LRRHPGQPL 
                 17 
               
               
                 23 
                 HPGQPLVRS 
                 16 
               
               
                 46 
                 KPRPAPLSC 
                 16 
               
               
                 44 
                 ASKPRPAPL 
                 15 
               
               
                 48 
                 RPAPLSCPR 
                 14 
               
               
                 26 
                 QPLVRSVPS 
                 13 
               
               
                 54 
                 CPRICCPSP 
                 13 
               
               
                 59 
                 CPSPQPGCP 
                 13 
               
               
                 8 
                 WPRSSWQNC 
                 12 
               
               
                 32 
                 VPSWSSSCG 
                 12 
               
               
                 50 
                 APLSCPRIC 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1 
               
               
                 610 aa, Part A, B08-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 44 
                 ASKPRPAPL 
                 28 
               
               
                 1 
                 MCKRRWHWP 
                 18 
               
               
                 67 
                 PPAYTGTVL 
                 17 
               
               
                 20 
                 LRRHPGQPL 
                 16 
               
               
                 26 
                 QPLVRSVPS 
                 16 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 
               
               
                 610 aa, Part A, B1510-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 22 
                 RHPGQPLVR 
                 14 
               
               
                 44 
                 ASKPRPAPL 
                 13 
               
               
                 67 
                 PPAYTGTVL 
                 13 
               
               
                 71 
                 TGTVLEQTL 
                 13 
               
               
                 6 
                 WHWPRSSWQ 
                 12 
               
               
                 20 
                 LRRHPGQPL 
                 11 
               
               
                 12 
                 SWQNCAFWL 
                 10 
               
               
                 10 
                 RSSWQNCAF 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXX 
               
               
                   
               
               
                 158P3D2 v.18, ORF: 2932-4764, Frame +1, 
               
               
                 610 aa, Part A, B2705-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 20 
                 LRRHPGQPL 
                 21 
               
               
                 21 
                 RRHPGQPLV 
                 20 
               
               
                 22 
                 RHPGQPLVR 
                 18 
               
               
                 48 
                 RPAPLSCPR 
                 17 
               
               
                 3 
                 KRRWHWPRS 
                 15 
               
               
                 4 
                 RRWHWPRSS 
                 15 
               
               
                 10 
                 RSSWQNCAF 
                 15 
               
               
                 29 
                 VRSVPSWSS 
                 14 
               
               
                 40 
                 GWAWASKPR 
                 14 
               
               
                 9 
                 PRSSWQNCA 
                 13 
               
               
                 14 
                 QNCAFWLRR 
                 13 
               
               
                 44 
                 ASKPRPAPL 
                 13 
               
               
                 47 
                 PRPAPLSCP 
                 13 
               
               
                 55 
                 PRICCPSPQ 
                 13 
               
               
                 67 
                 PPAYTGTVL 
                 13 
               
               
                 38 
                 SCGWAWASK 
                 12 
               
               
                 71 
                 TGTVLEQTL 
                 12 
               
               
                 2 
                 CKRRWHWPR 
                 11 
               
               
                 12 
                 SWQNCAFWL 
                 11 
               
               
                 13 
                 WQNCAFWLR 
                 11 
               
               
                 15 
                 NCAFWLRRH 
                 11 
               
               
                 49 
                 PAPLSCPRI 
                 11 
               
               
                 62 
                 PQPGCPPAY 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1 
               
               
                 610 aa, Part A, B2709-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 21 
                 RRHPGQPLV 
                 24 
               
               
                 20 
                 LRRHPGQPL 
                 20 
               
               
                 3 
                 KRRWHWPRS 
                 14 
               
               
                 4 
                 RRWHWPRSS 
                 14 
               
               
                 10 
                 RSSWQNCAF 
                 12 
               
               
                 44 
                 ASKPRPAPL 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1 610 aa, 
               
               
                 Part A, B4402 9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 44 
                 ASKPRPAPL 
                 18 
               
               
                 62 
                 PQPGCPPAY 
                 15 
               
               
                 10 
                 RSSWQNCAF 
                 13 
               
               
                 12 
                 SWQNCAFWL 
                 13 
               
               
                 35 
                 WSSSCGWAW 
                 13 
               
               
                 11 
                 SSWQNCAFW 
                 12 
               
               
                 27 
                 PLVRSVPSW 
                 12 
               
               
                 5 
                 RWHWPRSSW 
                 11 
               
               
                 67 
                 PPAYTGTVL 
                 11 
               
               
                 71 
                 TGTVLEQTL 
                 11 
               
               
                 20 
                 LRRHPGQPL 
                 10 
               
               
                 33 
                 PSWSSSCGW 
                 10 
               
               
                 49 
                 PAPLSCPRI 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 B5101-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 49 
                 PAPLSCPRI 
                 23 
               
               
                 66 
                 CPPAYTGTV 
                 23 
               
               
                 67 
                 PPAYTGTVL 
                 21 
               
               
                 24 
                 PGQPLVRSV 
                 17 
               
               
                 68 
                 PAYTGTVLE 
                 16 
               
               
                 71 
                 TGTVLEQTL 
                 15 
               
               
                 41 
                 WAWASKPRP 
                 14 
               
               
                 50 
                 APLSCPRIC 
                 14 
               
               
                 16 
                 CAFWLRRHP 
                 13 
               
               
                 23 
                 HPGQPLVRS 
                 13 
               
               
                 26 
                 QPLVRSVPS 
                 13 
               
               
                 32 
                 VPSWSSSCG 
                 12 
               
               
                 46 
                 KPRPAPLSC 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1 610 aa, 
               
               
                 Part A, A1-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 61 
                 S P QPGC P PAY 
                 21 
               
               
                 72 
                 G T VLEQ T LSP 
                 12 
               
               
                 21 
                 R R HPGQ P LVR 
                 11 
               
               
                 44 
                 A S KPRP A PLS 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1 610 aa, 
               
               
                 Part A, A0201 10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 73 
                 TVLEQ T LSPL 
                 22 
               
               
                 19 
                 WLRRH P GQPL 
                 20 
               
               
                 70 
                 YTGTV L EQTL 
                 18 
               
               
                 23 
                 HPGQP L VRSV 
                 16 
               
               
                 43 
                 WASKP R PAPL 
                 16 
               
               
                 65 
                 GCPPA Y TGTV 
                 15 
               
               
                 11 
                 SSWQN C AFWL 
                 13 
               
               
                 20 
                 LRRHP G QPLV 
                 13 
               
               
                 48 
                 RPAPL S CPRI 
                 13 
               
               
                 51 
                 PLSCP R ICCP 
                 11 
               
               
                 69 
                 AYTGT V LEQT 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1 
               
               
                 610 aa, Part A, A020310-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part A, A3-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 19 
                 WL R RH PG QPL 
                 19 
               
               
                 21 
                 RR H PG QP LVR 
                 19 
               
               
                 27 
                 PL V RS VP SWS 
                 18 
               
               
                 37 
                 SS C GW AW ASK 
                 18 
               
               
                 73 
                 TV L EQ TL SPL 
                 16 
               
               
                 28 
                 LV R SV PS WSS 
                 15 
               
               
                 31 
                 SV P SW SS SCG 
                 14 
               
               
                 56 
                 RI C CP SP QPG 
                 13 
               
               
                 30 
                 RS V PS WS SSC 
                 12 
               
               
                 46 
                 KP R PA PL SCP 
                 12 
               
               
                 61 
                 SP Q PG CP PAY 
                 12 
               
               
                 24 
                 PG Q PL VR SVP 
                 11 
               
               
                 4 
                 RR W HW PR SSW 
                 10 
               
               
                 18 
                 FW L RR HP GQP 
                 10 
               
               
                 22 
                 RH P GQ PL VRS 
                 10 
               
               
                 44 
                 AS K PR PA PLS 
                 10 
               
               
                 45 
                 SK P RP AP LSC 
                 10 
               
               
                 51 
                 PL S CP RI CCP 
                 10 
               
               
                 66 
                 CP P AY TG TVL 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1 610 aa, 
               
               
                 Part A, A26-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 73 
                 TVLEQTLSPL 
                 26 
               
               
                 70 
                 YTGTVLEQTL 
                 17 
               
               
                 72 
                 GTVLEQTLSP 
                 14 
               
               
                 31 
                 SVPSWSSSCG 
                 13 
               
               
                 61 
                 SPQPGCPPAY 
                 13 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part A, B0702 10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 66 
                 CPPAYTGTVL 
                 23 
               
               
                 48 
                 RPAPLSCPRI 
                 20 
               
               
                 8 
                 WPRSSWQNCA 
                 18 
               
               
                 23 
                 HPGQPLVRSV 
                 18 
               
               
                 43 
                 WASKPRPAPL 
                 15 
               
               
                 46 
                 KPRPAPLSCP 
                 14 
               
               
                 59 
                 CPSPQPGCPP 
                 14 
               
               
                 67 
                 PPAYTGTVLE 
                 14 
               
               
                 19 
                 WLRRHPGQPL 
                 13 
               
               
                 50 
                 APLSCPRICC 
                 13 
               
               
                 54 
                 CPRICCPSPQ 
                 13 
               
               
                 61 
                 SPQPGCPPAY 
                 13 
               
               
                 26 
                 QPLVRSVPSW 
                 12 
               
               
                 32 
                 VPSWSSSCGW 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XL 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part A, B08-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part A, B1510-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part A, B2705-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part A, B2709-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part A, B4402-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 61 
                 SPQPGCPPAY 
                 17 
               
               
                 34 
                 SWSSSCGWAW 
                 14 
               
               
                 9 
                 PRSSWQNCAF 
                 13 
               
               
                 11 
                 SSWQNCAFWL 
                 12 
               
               
                 26 
                 QPLVRSVPSW 
                 12 
               
               
                 43 
                 WASKPRPAPL 
                 12 
               
               
                 66 
                 CPPAYTGTVL 
                 12 
               
               
                 70 
                 YTGTVLEQTL 
                 12 
               
               
                 73 
                 TVLEQTLSPL 
                 12 
               
               
                 4 
                 RRWHWPRSSW 
                 11 
               
               
                 10 
                 RSSWQNCAFW 
                 11 
               
               
                 19 
                 WLRRHPGQPL 
                 11 
               
               
                 32 
                 VPSWSSSCGW 
                 11 
               
               
                 48 
                 RPAPLSCPRI 
                 11 
               
               
                 44 
                 ASKPRPAPLS 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part A, B5101-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part A, DRB1 0101-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is the 
               
               
                 start position plus fouteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 26 
                 QPLVRSVPSWSSSCG 
                 30 
               
               
                 17 
                 AFWLRRHPGQPLVRS 
                 26 
               
               
                 16 
                 CAFWLRRHPGQPLVR 
                 24 
               
               
                 25 
                 GQPLVRSVPSWSSSC 
                 23 
               
               
                 57 
                 ICCPSPQPGCPPAYT 
                 22 
               
               
                 71 
                 TGTVLEQTLSPLWDE 
                 22 
               
               
                 23 
                 HPGQPLVRSVPSWSS 
                 21 
               
               
                 69 
                 AYTGTVLEQTLSPLW 
                 19 
               
               
                 32 
                 VPSWSSSCGWAWASK 
                 18 
               
               
                 38 
                 SCGWAWASKPRPAPL 
                 18 
               
               
                 40 
                 GWAWASKPRPAPLSC 
                 18 
               
               
                 1 
                 MCKRRWHWPRSSWQN 
                 17 
               
               
                 36 
                 SSSCGWAWASKPRPA 
                 17 
               
               
                 42 
                 AWASKPRPAPLSCPR 
                 17 
               
               
                 64 
                 PGCPPAYTGTVLEQT 
                 17 
               
               
                 3 
                 KRRWHWPRSSWQNCA 
                 16 
               
               
                 9 
                 PRSSWQNCAFWLRRH 
                 16 
               
               
                 22 
                 RHPGQPLVRSVPSWS 
                 16 
               
               
                 34 
                 SWSSSCGWAWASKPR 
                 16 
               
               
                 41 
                 WAWASKPRPAPLSCP 
                 16 
               
               
                 46 
                 KPRPAPLSCPRICCP 
                 16 
               
               
                 49 
                 PAPLSCPRICCPSPQ 
                 16 
               
               
                 70 
                 YTGTVLEQTLSPLWD 
                 16 
               
               
                 39 
                 CGWAWASKPRPAPLS 
                 15 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part A, DRB1 0301-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is the start 
               
               
                 position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 17 
                 AFWLRRHPGQPLVRS 
                 21 
               
               
                 71 
                 TGTVLEQTLSPLWDE 
                 21 
               
               
                 7 
                 HWPRSSWQNCAFWLR 
                 16 
               
               
                 68 
                 PAYTGTVLEQTLSPL 
                 16 
               
               
                 26 
                 QPLVRSVPSWSSSCG 
                 13 
               
               
                 29 
                 VRSVPSWSSSCGWAW 
                 12 
               
               
                 54 
                 CPRICCPSPQPGCPP 
                 12 
               
               
                 72 
                 GTVLEQTLSPLWDEL 
                 12 
               
               
                 25 
                 GQPLVRSVPSWSSSC 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part A, DRB1 0401-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is the 
               
               
                 start position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 26 
                 QPLVRSVPSWSSSCG 
                 26 
               
               
                 3 
                 KRRWHWPRSSWQNCA 
                 22 
               
               
                 23 
                 HPGQPLVRSVPSWSS 
                 18 
               
               
                 63 
                 QPGCPPAYTGTVLEQ 
                 18 
               
               
                 69 
                 AYTGTVLEQTLSPLW 
                 18 
               
               
                 15 
                 NCAFWLRRHPGQPLV 
                 17 
               
               
                 38 
                 SCGWAWASKPRPAPL 
                 16 
               
               
                 67 
                 PPAYTGTVLEQTLSP 
                 16 
               
               
                 17 
                 AFWLRRHPGQPLVRS 
                 14 
               
               
                 25 
                 GQPLVRSVPSWSSSC 
                 14 
               
               
                 29 
                 VRSVPSWSSSCGWAW 
                 14 
               
               
                 71 
                 TGTVLEQTLSPLWDE 
                 14 
               
               
                 72 
                 GTVLEQTLSPLWDEL 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part A, DRB1 1101-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; 
               
               
                 each start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is 
               
               
                 the start position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 38 
                 SCGWAWASKPRPAPL 
                 26 
               
               
                 40 
                 GWAWASKPRPAPLSC 
                 24 
               
               
                 22 
                 RHPGQPLVRSVPSWS 
                 23 
               
               
                 14 
                 QNCAFWLRRHPGQPL 
                 21 
               
               
                 15 
                 NCAFWLRRHPGQPLV 
                 18 
               
               
                 26 
                 QPLVRSVPSWSSSCG 
                 18 
               
               
                 16 
                 CAFWLRRHPGQPLVR 
                 16 
               
               
                 69 
                 AYTGTVLEQTLSPLW 
                 16 
               
               
                 13 
                 WQNCAFWLRRHPGQP 
                 15 
               
               
                 25 
                 GQPLVRSVPSWSSSC 
                 14 
               
               
                 67 
                 PPAYTGTVLEQTLSP 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, A1-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 G I SRQL L KH 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, A0201-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 GISRQ L LKH 
                 16 
               
               
                 6 
                 LLKHN F DED 
                 13 
               
               
                 5 
                 QLLKH N FDE 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part B, A0203-9-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, A3-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 GI S RQ LL KH 
                 18 
               
               
                 5 
                 QL L KH NF DE 
                 14 
               
               
                 6 
                 LL K HN FD ED 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, A26-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 GISRQLLKH 
                 10 
               
               
                 3 
                 SRQLLKHNF 
                 10 
               
               
                 2 
                 ISRQLLKHN 
                 5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B0702-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 8 
                 KHNFDEDEM 
                 7 
               
               
                 3 
                 SRQLLKHNF 
                 6 
               
               
                 1 
                 GISRQLLKH 
                 4 
               
               
                 6 
                 L L K H N F D E D 
                 17 
               
               
                 4 
                 R Q L L K H N F D 
                 11 
               
               
                 3 
                 S R Q L L K H N F 
                 9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B08-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 6 
                 LLKHNFDED 
                 17 
               
               
                 4 
                 RQLLKHNFD 
                 11 
               
               
                 3 
                 SRQLLKHNF 
                 9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B1510-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 8 
                 KHNFDEDEM 
                 17 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B2705-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 3 
                 SRQLLKHNF 
                 25 
               
               
                 1 
                 GISRQLLKH 
                 15 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B2709-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 3 
                 SRQLLKHNF 
                 19 
               
               
                 8 
                 KHNFDEDEM 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B4402-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 3 
                 SRQLLKHNF 
                 13 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B5101 9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 ISRQLLKHN 
                 5 
               
               
                 1 
                 GISRQLLKH 
                 4 
               
               
                 4 
                 RQLLKHNFD 
                 4 
               
               
                 5 
                 QLLKHNFDE 
                 4 
               
               
                 6 
                 LLKHNFDED 
                 3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, A1-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 A G ISRQ L LKH 
                 9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, A0201-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 6 
                 QLLKH N FDED 
                 14 
               
               
                 2 
                 GISRQ L LKHN 
                 12 
               
               
                 1 
                 AGISR Q LLKH 
                 11 
               
               
                 7 
                 LLKHN F DEDE 
                 11 
               
               
                 8 
                 LKHNF D EDEM 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part B, A0203-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, A3-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 6 
                 QL L KH NF DED 
                 14 
               
               
                 1 
                 AG I SR QL LKH 
                 13 
               
               
                 2 
                 GI S RQ LL KHN 
                 11 
               
               
                 7 
                 LL K HN FD EDE 
                 11 
               
               
                 3 
                 IS R QL LK HNF 
                 9 
               
               
                 5 
                 RQ L LK HN FDE 
                 7 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, A26-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 AGISRQLLKH 
                 11 
               
               
                 2 
                 GISRQLLKHN 
                 8 
               
               
                 3 
                 ISRQLLKHNF 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B0702-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 3 
                 ISRQLLKHNF 
                 9 
               
               
                 8 
                 LKHNFDEDEM 
                 6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XL 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part B, B08-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLI 
               
               
                   
               
             
            
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part B, B1510-10-mers 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part B, DRB1 0101-15-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part B, B2705-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part B, B2709-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, B4402-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 3 
                 ISRQLLKHNF 
                 11 
               
               
                 1 
                 AGISRQLLKH 
                 9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part B, B5101-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, DRB1 0101-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 15 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 14 
                 KHNFDEDEMDDPGDS 
                 19 
               
               
                 6 
                 AGISRQLLKHNFDED 
                 18 
               
               
                 1 
                 PFLAEAGISRQLLKH 
                 15 
               
               
                 5 
                 EAGISRQLLKHNFDE 
                 15 
               
               
                 2 
                 FLAEAGISRQLLKHN 
                 14 
               
               
                 9 
                 SRQLLKHNFDEDEMD 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, DRB1 0301-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length 
               
               
                 of the peptide is 15 amino acids, and the 
               
               
                 end position for each peptide is the 
               
               
                 start position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 14 
                 KHNFDEDEMDDPGDS 
                 20 
               
               
                 2 
                 FLAEAGISRQLLKHN 
                 17 
               
               
                 6 
                 AGISRQLLKHNFDED 
                 17 
               
               
                 9 
                 SRQLLKHNFDEDEMD 
                 12 
               
               
                 5 
                 EAGISRQLLKHNFDE 
                 11 
               
               
                 10 
                 RQLLKHNFDEDEMDD 
                 11 
               
               
                 12 
                 LLKHNFDEDEMDDPG 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, DRB1 0401-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 15 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 FLAEAGISRQLLKHN 
                 18 
               
               
                 14 
                 KHNFDEDEMDDPGDS 
                 16 
               
               
                 5 
                 EAGISRQLLKHNFDE 
                 14 
               
               
                 1 
                 PFLAEAGISRQLLKH 
                 12 
               
               
                 3 
                 LAEAGISRQLLKHNF 
                 12 
               
               
                 7 
                 GISRQLLKHNFDEDE 
                 12 
               
               
                 11 
                 QLLKHNFDEDEMDDP 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part B, DRB1 1101-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 15 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 14 
                 KHNFDEDEMDDPGDS 
                 16 
               
               
                 2 
                 FLAEAGISRQLLKHN 
                 15 
               
               
                 6 
                 AGISRQLLKHNFDED 
                 14 
               
               
                 7 
                 GISRQLLKHNFDEDE 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, A1-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 5 
                 Q Y EVWV Q QG 
                 11 
               
               
                 3 
                 A S QYEV W VQ 
                 7 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIII 
               
               
                   
               
               
                 158P3D2-v.18 ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, A0201-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 LASQY E VWV 
                 19 
               
               
                 1 
                 GLASQ Y EVW 
                 13 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIV 
               
               
                   
               
               
                 158P3D2-v.18 ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part C, A0203-9-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, A3-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 GL A SQ YE VW 
                 15 
               
               
                 4 
                 SQ Y EV WV QQ 
                 14 
               
               
                 9 
                 WV Q QG PQ EP 
                 13 
               
               
                 7 
                 EV W VQ QG PQ 
                 11 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, A26-9mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 7 
                 EVWVQQGPQ 
                 21 
               
               
                 9 
                 WVQQGPQEP 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B0702-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 10 
                 VQQGPQEPF 
                 12 
               
               
                 2 
                 LASQYEVWV 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B08-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 GLASQYEVW 
                 7 
               
               
                 10 
                 VQQGPQEPF 
                 7 
               
               
                 2 
                 LASQYEVWV 
                 5 
               
               
                 4 
                 SQYEVWVQQ 
                 5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B1510-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 10 
                 VQQGPQEPF 
                 8 
               
               
                 1 
                 GLASQYEVW 
                 4 
               
               
                 2 
                 LASQYEVWV 
                 4 
               
               
                 9 
                 WVQQGPQEP 
                 4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B2705-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 10 
                 VQQGPQEPF 
                 15 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B2709-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end 
               
               
                 position for each peptide is the start 
               
               
                 position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 LASQYEVWV 
                 9 
               
               
                 10 
                 VQQGPQEPF 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B4402-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 GLASQYEVW 
                 11 
               
               
                 10 
                 VQQGPQEPF 
                 11 
               
               
                 6 
                 YEVWVQQGP 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B5101-9-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 9 amino acids, and the end position 
               
               
                 for each peptide is the start position plus eight. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 LASQYEVWV 
                 22 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, A1-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 6 
                 Q Y EVWV Q QGP 
                 10 
               
               
                 4 
                 A S QYEV W VQQ 
                 5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, A0201-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 GLASQ Y EVWV 
                 25 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part C, A0203-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, A3-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 GL A SQ YE VWV 
                 16 
               
               
                 8 
                 EV W VQ QG PQE 
                 16 
               
               
                 10 
                 WV Q QG PQ EPF 
                 14 
               
               
                 4 
                 AS Q YE VW VQQ 
                 10 
               
               
                 5 
                 SQ Y EV WV QQG 
                 9 
               
               
                 1 
                 CG L AS QY EVW 
                 8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, A26-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 8 
                 EVWVQQGPQE 
                 22 
               
               
                 10 
                 WVQQGPQEPF 
                 18 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XXXIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B0702-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 2 
                 GLASQYEVWV 
                 9 
               
               
                 10 
                 WVQQGPQEPF 
                 7 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XL 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part C, B08-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part C, B1510-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part C, B2705-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part C, B2709-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, B4402-10-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 10 amino acids, and the end position 
               
               
                 for each peptide is the start position plus nine. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 CGLASQYEVW 
                 12 
               
               
                 7 
                 YEVWVQQGPQ 
                 10 
               
               
                 10 
                 WVQQGPQEPF 
                 10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLV 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame + 1, 
               
               
                 610 aa, Part C, B5101-10-mers 
               
               
                   
               
             
            
               
                 No Results Found. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVI 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, DRB1 0101-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 15 amino acids, and the end position 
               
               
                 for each peptide is the start position plus 
               
               
                 fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 5 
                 NCGLASQYEVWVQQG 
                 22 
               
               
                 9 
                 ASQYEVWVQQGPQEP 
                 20 
               
               
                 2 
                 HRANCGLASQYEVWV 
                 14 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, DRB1 0301-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 15 amino acids, and the end position 
               
               
                 for each peptide is the start position plus 
               
               
                 fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 5 
                 NCGLASQYEVWVQQG 
                 18 
               
               
                 1 
                 HHRANCGLASQYEVW 
                 9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLVIII 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, DRB1 0401-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 15 amino acids, and the end position 
               
               
                 for each peptide is the start position plus 
               
               
                 fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 3 
                 RANCGLASQYEVWVQ 
                 18 
               
               
                 10 
                 SQYEVWVQQGPQEPF 
                 18 
               
               
                 9 
                 ASQYEVWVQQGPQEP 
                 16 
               
               
                 5 
                 NCGLASQYEVWVQQG 
                 14 
               
               
                 2 
                 HRANCGLASQYEVWV 
                 12 
               
               
                 7 
                 GLASQYEVWVQQGPQ 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE XLIX 
               
               
                   
               
               
                 158P3D2-v.18, ORF: 2932-4764, Frame +1, 610 aa, 
               
               
                 Part C, DRB1 1101-15-mers 
               
               
                 Each peptide is a portion of SEQ ID NO: 31; each 
               
               
                 start position is specified, the length of the 
               
               
                 peptide is 15 amino acids, and the end position 
               
               
                 for each peptide is the start position plus 
               
               
                 fourteen. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 9 
                 ASQYEVWVQQGPQEP 
                 19 
               
               
                 8 
                 LASQYEVWVQQGPQE 
                 12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE L 
               
             
            
               
                   
               
               
                 Protein Characteristics of 158P3D2 v.17 
               
            
           
           
               
               
               
               
            
               
                   
                 Bioinformatic 
                   
                   
               
               
                 158P3D2 var. 17 
                 Program 
                 URL on World Wide Web 
                 Outcome 
               
               
                   
               
               
                 Protein length 
                   
                   
                 2036 aa 
               
               
                 Transmembrane 
                 TM Pred 
                 .ch.embnet.org/ 
                 1 TM helix 2003-2020; N-terminus intracellular 
               
               
                 region 
                   
                   
                 (type II) 
               
               
                   
                 HMMTop 
                 .enzim.hu/hmmtop/ 
                 1 TM helix 2003-2022; N-terminus intracellular 
               
               
                   
                   
                   
                 (type II) 
               
               
                   
                 Sosui 
                 .genome.ad.jp/SOSui/ 
                 1 TM helix 1999-2021 
               
               
                   
                 TMHMM 
                 .cbs.dtu.dk/services/TMHMM 
                 1 TM helix 2000-2022; N-terminus extracellular 
               
               
                   
                   
                   
                 (type I) 
               
               
                 Signal Peptide 
                 Signal P 
                 .cbs.dtu.dk/services/SignalP/ 
                 None 
               
               
                 pI 
                 pI/MW tool 
                 .expasy.ch/tools/ 
                 5.64 
               
               
                 Molecular weight 
                 pI/MW tool 
                 .expasy.ch/tools/ 
                 227.6 kDa 
               
               
                 Localization 
                 PSORT II 
                 psort.nibb.ac.jp/ 
                 26% cytoplasmic, 17% vesicles of secretory 
               
               
                   
                   
                   
                 system, 13% nuclear, 13% mitochondrial, 8% 
               
               
                   
                   
                   
                 plasma membrane 
               
               
                 Motifs 
                 Pfam 
                 .sanger.ac.uk/Pfam/ 
                 C2 domain; amino acid transporter; 7TM 
               
               
                   
                   
                   
                 chemoreceptor; bradykinin; glycosyl hydrolase 
               
               
                   
                 Prints 
                 .biochem.ucl.ac.uk/ 
                 C2 domain; endolaptase 
               
               
                   
                 Blocks 
                 .blocks.fhcrc.org/ 
                 C2 domain 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LI 
               
             
            
               
                   
               
               
                 Exon composition of transcript 158P3D2 v.1 
               
            
           
           
               
               
               
            
               
                 Exon Number 
                 Start 
                 End 
               
               
                   
               
            
           
           
               
               
               
            
               
                 Exon 1 
                 1 
                 836 
               
               
                 Exon 2 
                 837 
                 922 
               
               
                 Exon 3 
                 923 
                 1021 
               
               
                 Exon 4 
                 1022 
                 1263 
               
               
                 Exon 5 
                 1264 
                 1547 
               
               
                 Exon 6 
                 1548 
                 1648 
               
               
                 Exon 7 
                 1649 
                 1961 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LII(a) 
               
               
                   
               
               
                 Nucleotide sequence of transcript variant 158P3D2 v.2 (SEQ ID NO: 335) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 atcaaggccc tgggctggag gaagacatcc cagatccaga ggagctcgac tgggggtcca 
                 60 
               
               
                   
               
               
                 agtactatgc gtcgctgcag gagctccagg ggcagcacaa ctttgatgaa gatgaaatgg 
                 120 
               
               
                   
               
               
                 atgatcctgg agattcagat ggggtcaacc tcatttctat ggttggggag atccaagacc 
                 180 
               
               
                   
               
               
                 agggtgaggc tgaagtcaaa ggcactgtgt ccccaaaaaa agcagttgcc accctgaaga 
                 240 
               
               
                   
               
               
                 tctacaacag gtccctggag gaagaattta accactttga agactggctg aatgtgtttc 
                 300 
               
               
                   
               
               
                 ctctgtaccg agggcaaggg ggccaggatg gaggtggaga agaggaagga tctggacacc 
                 360 
               
               
                   
               
               
                 ttgtgggcaa gttcaagggc tccttcctca tttaccctga atcagaggca gtgttgttct 
                 420 
               
               
                   
               
               
                 ctgagcccca gatctctcgg gggatcccac agaaccggcc catcaagctc ctggtcagag 
                 480 
               
               
                   
               
               
                 tgtatgttgt aaaggctacc aacctggctc ctgcagaccc caatggcaaa gcagaccctt 
                 540 
               
               
                   
               
               
                 acgtggtggt gagcgctggc cgggagcggc aggacaccaa ggaacgctac atccccaagc 
                 600 
               
               
                   
               
               
                 agctcaaccc catctttgga gagatcctgg agctaagcat ctctctccca gctgagacgg 
                 660 
               
               
                   
               
               
                 agctgacggt cgccgtattt gaacatgacc tcgtgggttc tgacgacctc atcggggaga 
                 720 
               
               
                   
               
               
                 cccacattga tctggaaaac cgattctata gccaccacag agcaaactgt gggctggcct 
                 780 
               
               
                   
               
               
                 cccagtatga agtgtgggtc cagcagggcc cacaggagcc attctgagtt tctggccaaa 
                 840 
               
               
                   
               
               
                 cacattcaag ctcacattcc cttttgtgtc tccagatcct atgatttcat ggaaggggac 
                 900 
               
               
                   
               
               
                 cctcccaccc accgccactg ccaaccaaga catagctcag tggtcaagac ttgggcttgg 
                 960 
               
               
                   
               
               
                 gagtcgggat cctgtaacga atgtcacttg accgctttct ttttttatga aacagtctcg 
                 1020 
               
               
                   
               
               
                 ctctgtctcc caggttggag tgcagtggca cgatctcggc tgactgcaac ctccacctcc 
                 1080 
               
               
                   
               
               
                 tgggttcaag cgattctcct gcctcagcct ccccagtagc tgggattaca ggcgtgggcc 
                 1140 
               
               
                   
               
               
                 cccatgtcca gctaattttt atattttcgc tctgtctccc aggttggagt gcagtggcac 
                 1200 
               
               
                   
               
               
                 gatctcggct gactgcaacc tccacctcct gggttcaagc gattctcctg cctcagcctc 
                 1260 
               
               
                   
               
               
                 cccagtagct gggattacag gcgtgggccc ccatgtccag ctaattttta tatttttagt 
                 1320 
               
               
                   
               
               
                 agagacaggg tttcaccatg ttgtccaggc tggtcttgaa cccctgacct caagtgatcc 
                 1380 
               
               
                   
               
               
                 acccacctct gcctcccaaa gtgctgggat tacaggtgtg agccaccatg ccaggccctc 
                 1440 
               
               
                   
               
               
                 ttaacctctt caagtctgtt ttctcatctg caaaacagag gtaataagat cagtatcttc 
                 1500 
               
               
                   
               
               
                 ttaatggaag cacctgggct acattttttt cattcattgt tatcataaat gaggactaac 
                 1560 
               
               
                   
               
               
                 ctgtctcccg ttgggagttt tgaacctaga cctcatgtct tcatgacgtc atcactgccc 
                 1620 
               
               
                   
               
               
                 caggcccagc tgtgtcccta caccagcccc agctgacgca tcttcttttt ctgcctgtag 
                 1680 
               
               
                   
               
               
                 agatggttac aatgcctggc gtgatgcatt ctggccttcg cagatcctgg cggggctgtg 
                 1740 
               
               
                   
               
               
                 ccaacgctgt ggcctccctg cccctgaata ccgagccggt gctgtcaagg tgggcagcaa 
                 1800 
               
               
                   
               
               
                 agtcttcctg acaccaccgg agaccctgcc cccagggatc tcttcacatg tggattgaca 
                 1860 
               
               
                   
               
               
                 tctttcctca agatgtgcct gctccacccc cagttgacat caagcctcgg cagccaatca 
                 1920 
               
               
                   
               
               
                 gctatgagct cagagttgtc atctggaaca cggaggatgt ggttctggat gacgagaatc 
                 1980 
               
               
                   
               
               
                 cactcaccgg agagatgtcg agtgacatct atgtgaagag ctgggtgaag gggttggagc 
                 2040 
               
               
                   
               
               
                 atgacaagca ggagacagac gttcacttca actccctgac tggggagggg aacttcaatt 
                 2100 
               
               
                   
               
               
                 ggcgctttgt gttccgcttt gactacctgc ccacggagcg ggaggtgagc gtctggcgca 
                 2160 
               
               
                   
               
               
                 ggtctggacc ctttgccctg gaggaggcgg agttccggca gcctgcagtg ctggtcctgc 
                 2220 
               
               
                   
               
               
                 aggatccctg gagttgcagc taccagacat ggtgcgtggg gcccggggcc ccgagctctg 
                 2280 
               
               
                   
               
               
                 ctctgtgcag ctggcccgca atggggccgg gccgaggtgc aatctgtttc gctgccgccg 
                 2340 
               
               
                   
               
               
                 cctgaggggc tggtggccgg tagtgaagct gaaggaggca gaggacgtgg agcgggaggc 
                 2400 
               
               
                   
               
               
                 gcaggaggct caggctggca agaagaagcg aaagcagagg aggaggaagg gccggccaga 
                 2460 
               
               
                   
               
               
                 agacctggag ttcacagaca tgggtggcaa tgtgtacatc ctcacgggca aggtggaggc 
                 2520 
               
               
                   
               
               
                 agagtttgag ctgctgactg tggaggaggc cgagaaacgg ccagtgggga aggggcggaa 
                 2580 
               
               
                   
               
               
                 gcagccagag cctctggaga aacccagccg ccccaaaact tccttcaact ggtttgtgaa 
                 2640 
               
               
                   
               
               
                 cccgctgaag acctttgtct tcttcatctg gcgccggtac tggcgcaccc tggtgctgct 
                 2700 
               
               
                   
               
               
                 gctactggtg ctgctcaccg tcttcctcct cctggtcttc tacaccatcc ctggccagat 
                 2760 
               
               
                   
               
               
                 cagccaggtc atcttccgtc ccctccacaa gtgactctcg ctgaccttgg acactcaccc 
                 2820 
               
               
                   
               
               
                 agggtgccaa cccttcaatg cctgctcctg gaagtctttc ttacccatgt gagctacccc 
                 2880 
               
               
                   
               
               
                 agagtctagt gcttcctctg aataaaccta tcacagcc 
                 2918 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIII (a) 
               
               
                   
               
               
                 Nucleotide sequence alignment of 121P1F1 v.1 (SEQ 
               
               
                 ID NO: 336) and 158P3D2 v.2 (SEQ ID NO: 337) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Score = 2348 bits (1221), Expect = 0.0 Identities = 1223/1224 (99%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 Score = 1340 bits (697), Expect = 0.0 Identities = 697/697 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIV(a) 
               
               
                   
               
               
                 Peptide sequences of protein coded by 158P3D2 v.2 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 &gt;158P3D2 v.2A (SEQ ID NO: 338) 
                   
               
               
                 MDDPGDSDGV NLISMVGEIQ DQGEAEVKGT VSPKKAVATL KIYNRSLEEE FNHFEDWLNV 
                 60 
               
               
                   
               
               
                 FPLYRGQGGQ DGGGEEEGSG HLVGKFKGSF LIYPESEAVL FSEPQISRGI PQNRPIKLLV 
                 120 
               
               
                   
               
               
                 RVYVVKATNL APADPNGKAD PYVVVSAGRE RQDTKERYIP KQLNPIFGEI LELSISLPAE 
                 180 
               
               
                   
               
               
                 TELTVAVFEH DLVGSDDLIG ETHIDLENRF YSHHRANCGL ASQYEVWVQQ GPQEPF 
                 236 
               
               
                   
               
               
                 &gt;158P3D2 v.2B (SEQ ID NO: 339) 
                   
               
               
                 MVRGARGPEL CSVQLARNGA GPRCNLFRCR RLRGWWPVVK LKEAEDVERE AQEAQAGKKK 
                 60 
               
               
                   
               
               
                 RKQRRRKGRP EDLEFTDMGG NVYILTGKVE AEFELLTVEE AEKRPVGKGR KQPEPLEKPS 
                 120 
               
               
                   
               
               
                 RPKTSFNWFV NPLKTFVFFI WRRYWRTLVL LLLVLLTVFL LLVFYTIPGQ ISQVIFRPLH 
                 180 
               
               
                   
               
               
                 K 
                 181 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LV(a) 
               
               
                   
               
               
                 Amino acid sequence alignment of 121P1F1 v.1 (SEQ ID NO: 340) and 
               
               
                 158P3D2 v.2 (SEQ ID NO: 341) 
               
               
                 Score = 372 bits (956), Expect = e−103Identities = 181/181 (100%), 
               
               
                 Positives = 181/181 (100%) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 V.1: 
                 148 
                 MVRGARGPELCSVQLARNGAGPRCNLFRCRRLRGWWPVVKLKEAEDVEREAQEAQAGKKK 
                 207 
               
               
                   
                   
                 MVRGARGPELCSVQLARNGAGPRCNLFRCRRLRGWWPVVKLKEAEDVEREAQEAQAGKKK 
                   
               
               
                 V.14: 
                 1 
                 MVRGARGPELCSVQLARNGAGPRCNLFRCRRLRGWWPVVKLKEAEDVEREAQEAQAGKKK 
                 60 
               
               
                   
               
               
                 V.1: 
                 208 
                 RKQRRRKGRPEDLEFTDMGGNVYILTGKVEAEFELLTVEEAEKRPVGKGRKQPEPLEKPS 
                 267 
               
               
                   
                   
                 RKQRRRKGRPEDLEFTDMGGNVYILTGKVEAEFELLTVEEAEKRPVGKGRKQPEPLEKPS 
                   
               
               
                 V.14: 
                 61 
                 RKQRRRKGRPEDLEFTDMGGNVYILTGKVEAEFELLTVEEAEKRPVGKGRKQPEPLEKPS 
                 120 
               
               
                   
               
               
                 V.1: 
                 268 
                 RPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLLVLLTVFLLLVFYTIPGQISQVIFRPLH 
                 327 
               
               
                   
                   
                 RPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLLVLLTVFLLLVFYTIPGQISQVIFRPLH 
                   
               
               
                 V.14: 
                 121 
                 RPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLLVLLTVFLLLVFYTIPGQISQVIFRPLH 
                 180 
               
               
                   
               
               
                 V.1: 
                 328 
                 K 
                 328 
               
               
                   
                   
                 K 
                   
               
               
                 V.14: 
                 181 
                 K 
                 181 
               
               
                   
               
               
                 Note: 
               
               
                 Protein variant 158P3D2 v.2A does not share common sequence with protein 158P3D2 v.1. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LII(b) 
               
               
                   
               
               
                 Nucleotide sequence of transcript variant 158P3D2 v.14 
               
               
                 (SEQ ID NO: 342) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 caggtgggcg ggctggtggg cagaagggca gacgggcaga ggaagtgcca gtgccactgg 
                 60 
               
               
                   
               
               
                 gaccatggct ctgacggtaa gcgtgcaacg actaacaggg ctgaccggca cccacgaccg 
                 120 
               
               
                   
               
               
                 acaagtgaag ctcacctttc gaggctttac ccagaaaaca agaaaaattc actgtggtcc 
                 180 
               
               
                   
               
               
                 agaagcagat atcggtgagc tgttccgatg gccccactat ggggctccac tggctgggga 
                 240 
               
               
                   
               
               
                 gtgtctgtct gtgcaggtgg tcaactgcag ccgtgtattc agccttaggc ctctagggac 
                 300 
               
               
                   
               
               
                 cctggtgatc tccctgcagc agctacagaa tgctgggcat ttggtgctac gggaagccct 
                 360 
               
               
                   
               
               
                 agtggatgag aatcttcaag tgtccccgat ccaggtggag cttgacctga agtaccagcc 
                 420 
               
               
                   
               
               
                 cccagagggc gctactggag cctggtcaga ggaggacttt ggggcaccca tccaggacag 
                 480 
               
               
                   
               
               
                 cttcgagtta atcatcccca atgtgggctt ccaggaactg gagcctgggg aggcccagct 
                 540 
               
               
                   
               
               
                 ggagcggcgg gcagtggctc taggccgcag gctagctcga agtctaggcc agcaggacga 
                 600 
               
               
                   
               
               
                 tgaagagaat gagctggagc ttgagctgga gcaggacctg gatgatgagc ctgacgtgga 
                 660 
               
               
                   
               
               
                 actttctggt gttatgttca gccccctcaa gagccgcgcc agggccctgg cccatgggga 
                 720 
               
               
                   
               
               
                 tcccttccag gtgtccagag ctcaagactt ccaggtggga gtcactgtgc tggaagccca 
                 780 
               
               
                   
               
               
                 gaaactggtg ggagtcaaca ttaaccccta tgtggccgtg caagtggggg ggcagcgccg 
                 840 
               
               
                   
               
               
                 tgtgaccgcc acacagcgtg ggaccagttg ccccttctac aatgagtact tcttgttcga 
                 900 
               
               
                   
               
               
                 atttcatgac acgcggcttc gtctccaaga cttgctgctg gagatcacgg tgagtggggt 
                 960 
               
               
                   
               
               
                 aggggtgacc agtgtccttc agagaagggg ggatgagaaa gctgcaggac taacaccacc 
                 1020 
               
               
                   
               
               
                 ttcccccaag gctttccatt cgcagaccct cccctttatg gccacccgga taggcacctt 
                 1080 
               
               
                   
               
               
                 caggatggac ctgggcatca tcttggacca gccagatggc cagttctacc aaagatgggt 
                 1140 
               
               
                   
               
               
                 tccgctgcat gatccccgag acacccgcgc cgggaccaag ggtttcatta aggtcacctt 
                 1200 
               
               
                   
               
               
                 gtccgtgagg gcgcgcgggg acctgccccc tccaatgcta cccccggccc cagggcactg 
                 1260 
               
               
                   
               
               
                 ttcggacatc gagaagaacc tgctcctgcc gcgcggggtg cccgccgaga ggccatgggc 
                 1320 
               
               
                   
               
               
                 gcggctccgc gtgcgcctgt accgcgccga ggggcttccc gcgctgcgcc tggggctgct 
                 1380 
               
               
                   
               
               
                 gggcagcctg gtccgcgccc tgcacgacca gcgcgtcctg gtggagccct atgtgcgggt 
                 1440 
               
               
                   
               
               
                 gtctttcctg gggcaggagg gcgagacgtc ggtgagcgcc gaggcggcgg cgcccgaatg 
                 1500 
               
               
                   
               
               
                 gaacgagcag ctgagcttcg tggagctctt cccgccgctg acgcgcagcc tccgcctgca 
                 1560 
               
               
                   
               
               
                 gctgcgggac gacgcgcccc tggtcgacgc ggcactcgct acgcacgtgc cggacctgag 
                 1620 
               
               
                   
               
               
                 gcggatctcc catccgggcc gcgcggcggg gtttaaccct accttcggcc cggcctgggt 
                 1680 
               
               
                   
               
               
                 gcccctctat ggctcgcccc ccggcgcggg gctccgggat agtcttcaag gtctcaacga 
                 1740 
               
               
                   
               
               
                 aggcgttggc caaggcattt ggttccgcgg ccgccttctg ctggctgtgt ccatgcaggt 
                 1800 
               
               
                   
               
               
                 gttggaaggg agagctgaac ctgagcctcc ccaggcccag caggggtcca cgttgtcccg 
                 1860 
               
               
                   
               
               
                 gctcacccga aagaagaaaa agaaagccag aagggatcag accccaaagg cggttccgca 
                 1920 
               
               
                   
               
               
                 gcacttggac gccagccccg gtgccgaggg gcctgagatc ccccgtgcca tggaggtgga 
                 1980 
               
               
                   
               
               
                 ggtggaggag ctgctgccgc tgccagagaa tgtcctggcg ccctgtgaag atttcctgct 
                 2040 
               
               
                   
               
               
                 tttcggtgtg ctcttcgagg ccaccatgat cgaccccacc gtggcctccc agcccatcag 
                 2100 
               
               
                   
               
               
                 cttcgagatc tccattggtc gcgcaggccg tctggaggag caattgggcc gagggtccag 
                 2160 
               
               
                   
               
               
                 ggctggggag ggaactgagg gtgcagccgt ggaggctcag cctctgctgg gagccaggcc 
                 2220 
               
               
                   
               
               
                 agaggaggag aaagaggagg aagaactggg gacccatgct cagcggcctg agcccatgga 
                 2280 
               
               
                   
               
               
                 cggcagtggg ccatacttct gcttgcccct ctgtcactgc aagccatgca tgcatgtgtg 
                 2340 
               
               
                   
               
               
                 gagttgctgg gaggaccaca cctggcgcct gcagagcagc aactgcgtgc gcaaagtggc 
                 2400 
               
               
                   
               
               
                 cgagaggctg gaccaggggc tgcaggaggt tgagagactg cagcgcaagc cggggcctgg 
                 2460 
               
               
                   
               
               
                 cgcctgtgca cagctcaagc aggcactgga agtactggtg gctgggagca gacagttttg 
                 2520 
               
               
                   
               
               
                 ccacggtgcc gagcgcagga cgatgacccg gcccaatgcc ctggatcgat gccgagggaa 
                 2580 
               
               
                   
               
               
                 actcctggtg cacagcctga accttttggc taagcaagga ctgcgacttc tacgcggcct 
                 2640 
               
               
                   
               
               
                 gagacggcgc aatgtgcaaa agaaggtggc actggccaag aagctcctgg caaaactgcg 
                 2700 
               
               
                   
               
               
                 ctttctggct gaggagcccc agccacccct ccccgatgtg ctggtctgga tgctcagcgg 
                 2760 
               
               
                   
               
               
                 gcagcgccgt gtggcctggg cccggatccc tgcccaggat gtgctgttct ctgtggttga 
                 2820 
               
               
                   
               
               
                 ggaggaacgg ggccgagact gtgggaagat ccagagtcta atgctcacgg cacccggggc 
                 2880 
               
               
                   
               
               
                 agcccctggt gaggtctgtg ccaagctgga gctcttcctg cggctgggcc tgggcaagca 
                 2940 
               
               
                   
               
               
                 agccaaggcc tgcacctctg agctgccccc ggatttgctg cccgagccct cagccgggct 
                 3000 
               
               
                   
               
               
                 gccctccagc ctacaccggg acgactttag ctacttccaa ctccgggctc acttgtacca 
                 3060 
               
               
                   
               
               
                 ggcccggggt gtgttggctg cagatgacag tggcctctcg gacccctttg ctcgagtcct 
                 3120 
               
               
                   
               
               
                 catctctacc cagtgtcaga ccacacgggt cctggagcag acgctgagcc ctctgtggga 
                 3180 
               
               
                   
               
               
                 tgaactcctg gtatttgagc agttgatcgt ggatgggagg agggagcacc tgcaggagga 
                 3240 
               
               
                   
               
               
                 gcctccatta gtgatcatca atgtatttga ccacaataag tttggccccc ccgtgttcct 
                 3300 
               
               
                   
               
               
                 gggcagggca ctggccgccc caagggtaaa gctgatggag gacccatacc aacgcccaga 
                 3360 
               
               
                   
               
               
                 gttgcagttc ttccccctga ggaagggacc ctgggcagcc ggagagctca ttgccgcctt 
                 3420 
               
               
                   
               
               
                 tcaactcatt gaactagact acagtggccg acttgagccc tcagtgccca gtgaggtgga 
                 3480 
               
               
                   
               
               
                 gccccaggat ctggcacccc tggttgagcc ccactctgga cgcctgtccc ttccacccaa 
                 3540 
               
               
                   
               
               
                 cgtgtgccca gtgctcaggg agttccgtgt tgaggtgctg ttctggggtc ttaggggact 
                 3600 
               
               
                   
               
               
                 tggtcgtgtg catctgctcg aggtggagca gccccaggtt gtactggagg tggctgggca 
                 3660 
               
               
                   
               
               
                 aggtgtggag tctgaggtcc tggccagcta ccgtgagagc cccaatttca ctgagcttgt 
                 3720 
               
               
                   
               
               
                 caggcatctg acagtggact tgccggagca gccttacttg cagcctccac tcagcatctt 
                 3780 
               
               
                   
               
               
                 ggtgattgag cgccgggcct ttggccacac agtccttgtg ggttcccaca ttgtccccca 
                 3840 
               
               
                   
               
               
                 catgctgcga ttcacatttc ggggtcatga ggatcctcct gaggaggaag gagagatgga 
                 3900 
               
               
                   
               
               
                 ggagacaggg gatatgatgc ccaagggacc tcaaggacag aagtccctgg atcccttctt 
                 3960 
               
               
                   
               
               
                 ggctgaagcg ggtatatcca gacagctcct gaagcctcct ctgaagaagc tcccactagg 
                 4020 
               
               
                   
               
               
                 aggcctccta aatcaaggcc ctgggctgga ggaagacatc ccagatccag aggagctcga 
                 4080 
               
               
                   
               
               
                 ctgggggtcc aagtactatg cgtcgctgca ggagctccag gggcagcaca actttgatga 
                 4140 
               
               
                   
               
               
                 agatgaaatg gatgatcctg gagattcaga tggggtcaac ctcatttcta tggttgggga 
                 4200 
               
               
                   
               
               
                 gatccaagac caggatctac aacaggtccc tgaaggaaga atttaaccac tttgaagact 
                 4260 
               
               
                   
               
               
                 ggctgaatgt gtttcctctg taccgagggc aagggggcca ggatggaggt ggagaagagg 
                 4320 
               
               
                   
               
               
                 aaggatctgg acaccttgtg ggcaagttca agggctcctt cctcatttac cctgaatcag 
                 4380 
               
               
                   
               
               
                 aggcagtgtt gttctctgag ccccagatct cccgggggat cccacagaac cggcccatca 
                 4440 
               
               
                   
               
               
                 agctcctggt cagagtgtat gttgtaaagg ctaccaacct ggctcctgca gaccccaatg 
                 4500 
               
               
                   
               
               
                 gcaaagcaga cccttacgtg gtggtgagcg ctggccggga gcggcaggac accaaggaac 
                 4560 
               
               
                   
               
               
                 gctacatccc caagcagctc aaccccatct ttggagagat cctggagcta agcatctctc 
                 4620 
               
               
                   
               
               
                 tcccagctga gacggagctg acggtcgccg tatttgatca tgacctcgtg ggttctgacg 
                 4680 
               
               
                   
               
               
                 acctcatcgg ggagacccac attgatctgg aaaaccgatt ctatagccac cacagagcaa 
                 4740 
               
               
                   
               
               
                 actgtgggct ggcctcccag tatgaagtgt gggtccagca gggcccacag gagccattct 
                 4800 
               
               
                   
               
               
                 gagtttctgg ccaaacacat tcaagctcac attccctttt gtgtctccag atcctatgat 
                 4860 
               
               
                   
               
               
                 ttcatggaag gggaccctcc cacccaccgc cactgccaac caagacatag ctcagtggtc 
                 4920 
               
               
                   
               
               
                 aagacttggg cttgggagtc gggatcctgt aacgaatgtc acttgaccgc tttctttttt 
                 4980 
               
               
                   
               
               
                 tatgaaacag tctcgctctg tctcccaggt tggagtgcag tggcacgatc tcggctgact 
                 5040 
               
               
                   
               
               
                 gcaacctcca cctcctgggt tcaagcgatt ctcctgcctc agcctcccca gtagctggga 
                 5100 
               
               
                   
               
               
                 ttacaggcgt gggcccccat gtccagctaa tttttatatt ttcgctctgt ctcccaggtt 
                 5160 
               
               
                   
               
               
                 ggagtgcagt ggcacgatct cggctgactg caacctccac ctcctgggtt caagcgattc 
                 5220 
               
               
                   
               
               
                 tcctgcctca gcctccccag tagctgggat tacaggcgtg ggcccccatg tccagctaat 
                 5280 
               
               
                   
               
               
                 ttttatattt ttagtagaga cagggtttca ccatgttgtc caggctggtc ttgaacccct 
                 5340 
               
               
                   
               
               
                 gacctcaagt gatccaccca cctctgcctc ccaaagtgct gggattacag gtgtgagcca 
                 5400 
               
               
                   
               
               
                 ccatgccagg ccctcttaac ctcttcaagt ctgttttctc atctgcaaaa cagaggtaat 
                 5460 
               
               
                   
               
               
                 aagatcagta tcttcttaat ggaagcacct ggactacatt tttttcattc attgttatca 
                 5520 
               
               
                   
               
               
                 taaatgagga ctaacctgtc tcccgttggg agttttgaac ctagacctca tgtcttcatg 
                 5580 
               
               
                   
               
               
                 acgtcatcac tgccccaggc ccagctgtgt ccctacacca gccccagctg acgcatcttc 
                 5640 
               
               
                   
               
               
                 tttttctgcc tgtagagatg gttacaatgc ctggcgtgat gcattctggc cttcgcagat 
                 5700 
               
               
                   
               
               
                 cctggcgggg ctgtgccaac gctgtggcct ccctgcccct gaataccgag ccggtgctgt 
                 5760 
               
               
                   
               
               
                 caaggtgggc agcaaagtct tcctgacacc accggagacc ctgcccccag ggatctcttc 
                 5820 
               
               
                   
               
               
                 acatgtggat tgacatcttt cctcaagatg tgcctgctcc acccccagtt gacatcaagc 
                 5880 
               
               
                   
               
               
                 ctcggcagcc aatcagctat gagctcagag ttgtcatctg gaacacggag gatgtggttc 
                 5940 
               
               
                   
               
               
                 tggatgacga gaatccactc accggagaga tgtcgagtga catctatgtg aagaggtagg 
                 6000 
               
               
                   
               
               
                 ctgctggccg ggcggggcaa cggcggtgca ctagggggat tgcaaatggg tgtgggccct 
                 6060 
               
               
                   
               
               
                 cgggctgagt ccagagcccc gaccccaggc cctccgtggt gctgagagcg gggtgaggag 
                 6120 
               
               
                   
               
               
                 tgggttctcc atgtagctcc agccctgacg ctcacccacc ccggccccag ctgggtgaag 
                 6180 
               
               
                   
               
               
                 gggttggagc atgacaagca ggagacagac gttcacttca actccctgac tggggagggg 
                 6240 
               
               
                   
               
               
                 aacttcaatt ggcgctttgt gttccgcttt gactacctgc ccacggagcg ggaggtgagc 
                 6300 
               
               
                   
               
               
                 gtctggcgca ggtctggacc ctttgccctg gaggaggcgg agttccggca gcctgcagtg 
                 6360 
               
               
                   
               
               
                 ctggtcctgc aggtctggga ctatgaccgc atctctgcca atgacttcct tggatccctg 
                 6420 
               
               
                   
               
               
                 gagttgcagc taccagacat ggtgcgtggg gcccggggcc ccgagctctg ctctgtgcag 
                 6480 
               
               
                   
               
               
                 ctggcccgca atggggccgg gccgaggtgc aatctgtttc gctgccgccg cctgaggggc 
                 6540 
               
               
                   
               
               
                 tggtggccgg tagtgaagct gaaggaggca gaggacgtgg agcgggaggc gcaggaggct 
                 6600 
               
               
                   
               
               
                 caggctggca agaagaagcg aaagcagagg aggaggaagg gccggccaga agacctggag 
                 6660 
               
               
                   
               
               
                 ttcacagaca tgggtggcaa tgtgtacatc ctcacgggca aggtggaggc agagtttgag 
                 6720 
               
               
                   
               
               
                 ctgctgactg tggaggaggc cgagaaacgg ccagtgggga aggggcggaa gcagccagag 
                 6780 
               
               
                   
               
               
                 cctctggaga aacccagccg ccccaaaact tccttcaact ggtttgtgaa cccgctgaag 
                 6840 
               
               
                   
               
               
                 acctttgtct tcttcatctg gcgccggtac tggcgcaccc tggtgctgct gctactggtg 
                 6900 
               
               
                   
               
               
                 ctgctcaccg tcttcctcct cctggtcttc tacaccatcc ctggccagat cagccaggtc 
                 6960 
               
               
                   
               
               
                 atcttccgtc ccctccacaa gtgactctcg ctgaccttgg acactcaccc agggtgccaa 
                 7020 
               
               
                   
               
               
                 cccttcaatg cctgctcctg gaagtctttc ttacccatgt gagctacccc agagtctagt 
                 7080 
               
               
                   
               
               
                 gcttcctctg aataaaccta tcacagcc 
                 7108 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIII (b)  
               
               
                   
               
               
                 Nucleotide sequence alignment of 121P1F1 v.1 (SEQ 
               
               
                 ID NO: 343) and 158P3D2 v.14 (SEQ ID NO: 344) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Score = 1946 bits (1012), Expect = 0.0 Identities = 1018/1021 (99%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 Score = 1796 bits (934), Expect = 0.0 Identities = 938/940 (99%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIV(b) 
               
               
                   
               
               
                 Peptide sequences of protein coded by 158P3D2 v.14 (SEQ ID NO: 345) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 MALTVSVQRL TGLTGTHDRQ VKLTFRGFTQ KTRKIHCGPE ADIGELFRWP HYGAPLAGEC 
                 60 
               
               
                   
               
               
                 LSVQVVNCSR VFSLRPLGTL VISLQQLQNA GHLVLREALV DENLQVSPIQ VELDLKYQPP 
                 120 
               
               
                   
               
               
                 EGATGAWSEE DFGAPIQDSF ELIIPNVGFQ ELEPGEAQLE RRAVALGRRL ARSLGQQDDE 
                 180 
               
               
                   
               
               
                 ENELELELEQ DLDDEPDVEL SGVMFSPLKS RARALAHGDP FQVSRAQDFQ VGVTVLEAQK 
                 240 
               
               
                   
               
               
                 LVGVNINPYV AVQVGGQRRV TATQRGTSCP FYNEYFLFEF HDTRLRLQDL LLEITVSGVG 
                 300 
               
               
                   
               
               
                 VTSVLQRRGD EKAAGLTPPS PKAFHSQTLP FMATRIGTFR MDLGIILDQP DGQFYQRWVP 
                 360 
               
               
                   
               
               
                 LHDPRDTRAG TKGFIKVTLS VRARGDLPPP MLPPAPGHCS DIEKNLLLPR GVPAERPWAR 
                 420 
               
               
                   
               
               
                 LRVRLYRAEG LPALRLGLLG SLVRALHDQR VLVEPYVRVS FLGQEGETSV SAEAAAPEWN 
                 480 
               
               
                   
               
               
                 EQLSFVELFP PLTRSLRLQL RDDAPLVDAA LATHVPDLRR ISHPGRAAGF NPTFGPAWVP 
                 540 
               
               
                   
               
               
                 LYGSPPGAGL RDSLQGLNEG VGQGIWFRGR LLLAVSMQVL EGRAEPEPPQ AQQGSTLSRL 
                 600 
               
               
                   
               
               
                 TRKKKKKARR DQTPKAVPQH LDASPGAEGP EIPRAMEVEV EELLPLPENV LAPCEDFLLF 
                 660 
               
               
                   
               
               
                 GVLFEATMID PTVASQPISF EISIGRAGRL EEQLGRGSRA GEGTEGAAVE AQPLLGARPE 
                 720 
               
               
                   
               
               
                 EEKEEEELGT HAQRPEPMDG SGPYFCLPLC HCKPCMHVWS CWEDHTWRLQ SSNCVRKVAE 
                 780 
               
               
                   
               
               
                 RLDQGLQEVE RLQRKPGPGA CAQLKQALEV LVAGSRQFCH GAERRTMTRP NALDRCRGKL 
                 840 
               
               
                   
               
               
                 LVHSLNLLAK QGLRLLRGLR RRNVQKKVAL AKKLLAKLRF LAEEPQPPLP DVLVWMLSGQ 
                 900 
               
               
                   
               
               
                 RRVAWARIPA QDVLFSVVEE ERGRDCGKIQ SLMLTAPGAA PGEVCAKLEL FLRLGLGKQA 
                 960 
               
               
                   
               
               
                 KACTSELPPD LLPEPSAGLP SSLHRDDFSY FQLRAHLYQA RGVLAADDSG LSDPFARVLI 
                 1020 
               
               
                   
               
               
                 STQCQTTRVL EQTLSPLWDE LLVFEQLIVD GRREHLQEEP PLVIINVFDH NKFGPPVFLG 
                 1080 
               
               
                   
               
               
                 RALAAPRVKL MEDPYQRPEL QFFPLRKGPW AAGELIAAFQ LIELDYSGRL EPSVPSEVEP 
                 1140 
               
               
                   
               
               
                 QDLAPLVEPH SGRLSLPPNV CPVLREFRVE VLFWGLRGLG RVHLLEVEQP QVVLEVAGQG 
                 1200 
               
               
                   
               
               
                 VESEVLASYR ESPNFTELVR HLTVDLPEQP YLQPPLSILV IERRAFGHTV LVGSHIVPHM 
                 1260 
               
               
                   
               
               
                 LRFTFRGHED PPEEEGEMEE TGDMMPKGPQ GQKSLDPFLA EAGISRQLLK PPLKKLPLGG 
                 1320 
               
               
                   
               
               
                 LLNQGPGLEE DIPDPEELDW GSKYYASLQE LQGQHNFDED EMDDPGDSDG VNLISMVGEI 
                 1380 
               
               
                   
               
               
                 QDQDLQQVPE GRI 
                 1393 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LV(b) 
               
               
                   
               
               
                 Amino acid sequence alignment of 121P1F1 v.1 and 158P3D2 v.14 
               
               
                   
               
             
            
               
                 No significant similarity 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LII(c) 
               
               
                   
               
               
                 Nucleotide sequence of transcript variant 158P3D2 v.15 
               
               
                 (SEQ ID NO: 346) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 caggtgggcg ggctggtggg cagaagggca gacgggcaga ggaagtgcca gtgccactgg 
                 60 
               
               
                   
               
               
                 gaccatggct ctgacggtaa gcgtgcaacg actaacaggg ctgaccggca cccacgaccg 
                 120 
               
               
                   
               
               
                 acaagtgaag ctcacctttc gaggctttac ccagaaaaca agaaaaattc actgtggtcc 
                 180 
               
               
                   
               
               
                 agaagcagat atcggtgagc tgttccgatg gccccactat ggggctccac tggctgggga 
                 240 
               
               
                   
               
               
                 gtgtctgtct gtgcaggtgg tcaactgcag ccgtgtattc agccttaggc ctctagggac 
                 300 
               
               
                   
               
               
                 cctggtgatc tccctgcagc agctacagaa tgctgggcat ttggtgctac gggaagccct 
                 360 
               
               
                   
               
               
                 agtggatgag aatcttcaag tgtccccgat ccaggtggag cttgacctga agtaccagcc 
                 420 
               
               
                   
               
               
                 cccagagggc gctactggag cctggtcaga ggaggacttt ggggcaccca tccaggacag 
                 480 
               
               
                   
               
               
                 cttcgagtta atcatcccca atgtgggctt ccaggaactg gagcctgggg aggcccagct 
                 540 
               
               
                   
               
               
                 ggagcggcgg gcagtggctc taggccgcag gctagctcga agtctaggcc agcaggacga 
                 600 
               
               
                   
               
               
                 tgaagagaat gagctggagc ttgagctgga gcaggacctg gatgatgagc ctgacgtgga 
                 660 
               
               
                   
               
               
                 actttctggt gttatgttca gccccctcaa gagccgcgcc agggccctgg cccatgggga 
                 720 
               
               
                   
               
               
                 tcccttccag gtgtccagag ctcaagactt ccaggtggga gtcactgtgc tggaagccca 
                 780 
               
               
                   
               
               
                 gaaactggtg ggagtcaaca ttaaccccta tgtggccgtg caagtggggg ggcagcgccg 
                 840 
               
               
                   
               
               
                 tgtgaccgcc acacagcgtg ggaccagttg ccccttctac aatgagtact tcttgttcga 
                 900 
               
               
                   
               
               
                 atttcatgac acgcggcttc gtctccaaga cttgctgctg gagatcacgg tgagtggggt 
                 960 
               
               
                   
               
               
                 aggggtgacc agtgtccttc agagaagggg ggatgagaaa gctgcaggac taacaccacc 
                 1020 
               
               
                   
               
               
                 ttcccccaag gctttccatt cgcagaccct cccctttatg gccacccgga taggcacctt 
                 1080 
               
               
                   
               
               
                 caggatggac ctgggcatca tcttggacca gccagatggc cagttctacc aaagatgggt 
                 1140 
               
               
                   
               
               
                 tccgctgcat gatccccgag acacccgcgc cgggaccaag ggtttcatta aggtcacctt 
                 1200 
               
               
                   
               
               
                 gtccgtgagg gcgcgcgggg acctgccccc tccaatgcta cccccggccc cagggcactg 
                 1260 
               
               
                   
               
               
                 ttcggacatc gagaagaacc tgctcctgcc gcgcggggtg cccgccgaga ggccatgggc 
                 1320 
               
               
                   
               
               
                 gcggctccgc gtgcgcctgt accgcgccga ggggcttccc gcgctgcgcc tggggctgct 
                 1380 
               
               
                   
               
               
                 gggcagcctg gtccgcgccc tgcacgacca gcgcgtcctg gtggagccct atgtgcgggt 
                 1440 
               
               
                   
               
               
                 gtctttcctg gggcaggagg gcgagacgtc ggtgagcgcc gaggcggcgg cgcccgaatg 
                 1500 
               
               
                   
               
               
                 gaacgagcag ctgagcttcg tggagctctt cccgccgctg acgcgcagcc tccgcctgca 
                 1560 
               
               
                   
               
               
                 gctgcgggac gacgcgcccc tggtcgacgc ggcactcgct acgcacgtgc cggacctgag 
                 1620 
               
               
                   
               
               
                 gcggatctcc catccgggcc gcgcggcggg gtttaaccct accttcggcc cggcctgggt 
                 1680 
               
               
                   
               
               
                 gcccctctat ggctcgcccc ccggcgcggg gctccgggat agtcttcaag gtctcaacga 
                 1740 
               
               
                   
               
               
                 aggcgttggc caaggcattt ggttccgcgg ccgccttctg ctggctgtgt ccatgcaggt 
                 1800 
               
               
                   
               
               
                 gttggaaggg agagctgaac ctgagcctcc ccaggcccag caggggtcca cgttgtcccg 
                 1860 
               
               
                   
               
               
                 gctcacccga aagaagaaaa agaaagccag aagggatcag accccaaagg cggttccgca 
                 1920 
               
               
                   
               
               
                 gcacttggac gccagccccg gtgccgaggg gcctgagatc ccccgtgcca tggaggtgga 
                 1980 
               
               
                   
               
               
                 ggtggaggag ctgctgccgc tgccagagaa tgtcctggcg ccctgtgaag atttcctgct 
                 2040 
               
               
                   
               
               
                 tttcggtgtg ctcttcgagg ccaccatgat cgaccccacc gtggcctccc agcccatcag 
                 2100 
               
               
                   
               
               
                 cttcgagatc tccattggtc gcgcaggccg tctggaggag caattgggcc gagggtccag 
                 2160 
               
               
                   
               
               
                 ggctggggag ggaactgagg gtgcagccgt ggaggctcag cctctgctgg gagccaggcc 
                 2220 
               
               
                   
               
               
                 agaggaggag aaagaggagg aagaactggg gacccatgct cagcggcctg agcccatgga 
                 2280 
               
               
                   
               
               
                 cggcagtggg ccatacttct gcttgcccct ctgtcactgc aagccatgca tgcatgtgtg 
                 2340 
               
               
                   
               
               
                 gagttgctgg gaggaccaca cctggcgcct gcagagcagc aactgcgtgc gcaaagtggc 
                 2400 
               
               
                   
               
               
                 cgagaggctg gaccaggggc tgcaggaggt tgagagactg cagcgcaagc cggggcctgg 
                 2460 
               
               
                   
               
               
                 cgcctgtgca cagctcaagc aggcactgga agtactggtg gctgggagca gacagttttg 
                 2520 
               
               
                   
               
               
                 ccacggtgcc gagcgcagga cgatgacccg gcccaatgcc ctggatcgat gccgagggaa 
                 2580 
               
               
                   
               
               
                 actcctggtg cacagcctga accttttggc taagcaagga ctgcgacttc tacgcggcct 
                 2640 
               
               
                   
               
               
                 gagacggcgc aatgtgcaaa agaaggtggc actggccaag aagctcctgg caaaactgcg 
                 2700 
               
               
                   
               
               
                 ctttctggct gaggagcaca actttgatga agatgaaatg gatgatcctg gagattcaga 
                 2760 
               
               
                   
               
               
                 tggggtcaac ctcatttcta tggttgggga gatccaagac cagggtgagg ctgaagtcaa 
                 2820 
               
               
                   
               
               
                 aggcactgtg tccccaaaaa aagcagttgc caccctgaag atctacaaca ggtccctgaa 
                 2880 
               
               
                   
               
               
                 ggaagaattt aaccactttg aagactggct gaatgtgttt cctctgtacc gagggcaagg 
                 2940 
               
               
                   
               
               
                 gggccaggat ggaggtggag aagaggaagg atctggacac cttgtgggca agttcaaggg 
                 3000 
               
               
                   
               
               
                 ctccttcctc atttaccctg aatcagaggc agtgttgttc tctgagcccc agatctcccg 
                 3060 
               
               
                   
               
               
                 ggggatccca cagaaccggc ccatcaagct cctggtcaga gtgtatgttg taaagctaag 
                 3120 
               
               
                   
               
               
                 aaacctttgc aaaatccaag gtcatgaaga cttttgcctg ttttctgctg ctaccaacct 
                 3180 
               
               
                   
               
               
                 ggctcctgca gaccccaatg gcaaagcaga cccttacgtg gtggtgagcg ctggccggga 
                 3240 
               
               
                   
               
               
                 gcggcaggac accaaggaac gctacatccc caagcagctc aaccccatct ttggagagat 
                 3300 
               
               
                   
               
               
                 cctggagcta agcatctctc tcccagctga gacggagctg acggtcgccg tatttgatca 
                 3360 
               
               
                   
               
               
                 tgacctcgtg ggttctgacg acctcatcgg ggagacccac attgatctgg aaaaccgatt 
                 3420 
               
               
                   
               
               
                 ctatagccac cacagagcaa actgtgggct ggcctcccag tatgaagtgt gggtccagca 
                 3480 
               
               
                   
               
               
                 gggcccacag gagccattct gagtttctgg ccaaacacat tcaagctcac attccctttt 
                 3540 
               
               
                   
               
               
                 gtgtctccag atcctatgat ttcatggaag gggaccctcc cacccaccgc cactgccaac 
                 3600 
               
               
                   
               
               
                 caagacatag ctcagtggtc aagacttggg cttgggagtc gggatcctgt aacgaatgtc 
                 3660 
               
               
                   
               
               
                 acttgaccgc tttctttttt tatgaaacag tctcgctctg tctcccaggt tggagtgcag 
                 3720 
               
               
                   
               
               
                 tggcacgatc tcggctgact gcaacctcca cctcctgggt tcaagcgatt ctcctgcctc 
                 3780 
               
               
                   
               
               
                 agcctcccca gtagctggga ttacaggcgt gggcccccat gtccagctaa tttttatatt 
                 3840 
               
               
                   
               
               
                 ttcgctctgt ctcccaggtt ggagtgcagt ggcacgatct cggctgactg caacctccac 
                 3900 
               
               
                   
               
               
                 ctcctgggtt caagcgattc tcctgcctca gcctccccag tagctgggat tacaggcgtg 
                 3960 
               
               
                   
               
               
                 ggcccccatg tccagctaat ttttatattt ttagtagaga cagggtttca ccatgttgtc 
                 4020 
               
               
                   
               
               
                 caggctggtc ttgaacccct gacctcaagt gatccaccca cctctgcctc ccaaagtgct 
                 4080 
               
               
                   
               
               
                 gggattacag gtgtgagcca ccatgccagg ccctcttaac ctcttcaagt ctgttttctc 
                 4140 
               
               
                   
               
               
                 atctgcaaaa cagaggtaat aagatcagta tcttcttaat ggaagcacct ggactacatt 
                 4200 
               
               
                   
               
               
                 tttttcattc attgttatca taaatgagga ctaacctgtc tcccgttggg agttttgaac 
                 4260 
               
               
                   
               
               
                 ctagacctca tgtcttcatg acgtcatcac tgccccaggc ccagctgtgt ccctacacca 
                 4320 
               
               
                   
               
               
                 gccccagctg acgcatcttc tttttctgcc tgtagagatg gttacaatgc ctggcgtgat 
                 4380 
               
               
                   
               
               
                 gcattctggc cttcgcagat cctggcgggg ctgtgccaac gctgtggcct ccctgcccct 
                 4440 
               
               
                   
               
               
                 gaataccgag ccggtgctgt caaggtgggc agcaaagtct tcctgacacc accggagacc 
                 4500 
               
               
                   
               
               
                 ctgcccccag ggatctcttc acatgtggat tgacatcttt cctcaagatg tgcctgctcc 
                 4560 
               
               
                   
               
               
                 acccccagtt gacatcaagc ctcggcagcc aatcagctat gagctcagag ttgtcatctg 
                 4620 
               
               
                   
               
               
                 gaacacggag gatgtggttc tggatgacga gaatccactc accggagaga tgtcgagtga 
                 4680 
               
               
                   
               
               
                 catctatgtg aagaggtagg ctgctggccg ggcggggcaa cggcggtgca ctagggggat 
                 4740 
               
               
                   
               
               
                 tgcaaatggg tgtgggccct cgggctgagt ccagagcccc gaccccaggc cctccgtggt 
                 4800 
               
               
                   
               
               
                 gctgagagcg gggtgaggag tgggttctcc atgtagctcc agccctgacg ctcacccacc 
                 4860 
               
               
                   
               
               
                 ccggccccag ctgggtgaag gggttggagc atgacaagca ggagacagac gttcacttca 
                 4920 
               
               
                   
               
               
                 actccctgac tggggagggg aacttcaatt ggcgctttgt gttccgcttt gactacctgc 
                 4980 
               
               
                   
               
               
                 ccacggagcg ggaggtgagc gtctggcgca ggtctggacc ctttgccctg gaggaggcgg 
                 5040 
               
               
                   
               
               
                 agttccggca gcctgcagtg ctggtcctgc aggtctggga ctatgaccgc atctctgcca 
                 5100 
               
               
                   
               
               
                 atgacttcct tggtattaca atgcttagcc ttccccaccc tcagcccctg cctccagccc 
                 5160 
               
               
                   
               
               
                 tcacctccgc ccctgcctcc agccctcact tccgtccccc agttccctac tctgacccaa 
                 5220 
               
               
                   
               
               
                 ccttgaatct tgggattttg gacccgaggt gtgaaacctt tgctttctgg cctaattact 
                 5280 
               
               
                   
               
               
                 gagttaatta ggcctagacc acagtaacct ccattcccac ccagagtctc tgattcaact 
                 5340 
               
               
                   
               
               
                 ctgatttgac cctagcttgt caccctgaca ccgactccac agcctttggt ccttggcact 
                 5400 
               
               
                   
               
               
                 ctgatcccga cccttggccc tcttccactg ggaagtagca atgggtggac cgctgggctg 
                 5460 
               
               
                   
               
               
                 tggtctgggt ggtctatagc tgtggcctga ccgcacactg caacaacttt caatgcccca 
                 5520 
               
               
                   
               
               
                 atttacaacc ttggtgtgtt gcctcctcac ccctggcaca atgagacttt gatcccatgc 
                 5580 
               
               
                   
               
               
                 ctaatctggt gtgctctgga cttgcaggat ccctggagtt gcagctacca gacatggtgc 
                 5640 
               
               
                   
               
               
                 gtggggcccg gggccccgag ctctgctctg tgcagctggc ccgcaatggg gccgggccga 
                 5700 
               
               
                   
               
               
                 ggtgcaatct gtttcgctgc cgccgcctga ggggctggtg gccggtagtg aagctgaagg 
                 5760 
               
               
                   
               
               
                 aggcagagga cgtggagcgg gaggcgcagg aggctcaggc tggcaagaag aagcgaaagc 
                 5820 
               
               
                   
               
               
                 agaggaggag gaagggccgg ccagaagacc tggagttcac agacatgggt ggcaatgtgt 
                 5880 
               
               
                   
               
               
                 acatcctcac gggcaaggtg gaggcagagt ttgagctgct gactgtggag gaggccgaga 
                 5940 
               
               
                   
               
               
                 aacggccagt ggggaagggg cggaagcagc cagagcctct ggagaaaccc agccgcccca 
                 6000 
               
               
                   
               
               
                 aaacttcctt caactggttt gtgaacccgc tgaagacctt tgtcttcttc atctggcgcc 
                 6060 
               
               
                   
               
               
                 ggtactggcg caccctggtg ctgctgctac tggtgctgct caccgtcttc ctcctcctgg 
                 6120 
               
               
                   
               
               
                 tcttctacac catccctggc cagatcagcc aggtcatctt ccgtcccctc cacaagtgac 
                 6180 
               
               
                   
               
               
                 tctcgctgac cttggacact cacccagggt gccaaccctt caatgcctgc tcctggaagt 
                 6240 
               
               
                   
               
               
                 ctttcttacc catgtgagct accccagagt ctagtgcttc ctctgaataa acctatcaca 
                 6300 
               
               
                   
               
               
                 gcc 
                 6303 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIII (c)  
               
               
                   
               
               
                 Nucleotide sequence alignment of 121P1F1 v.1 (SEQ 
               
               
                 ID NO: 347) and 158P3D2 v.15 (SEQ ID NO: 348) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Score = 1963 bits (1021), Expect = 0.0 Identities = 1021/1021 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 Score = 1340 bits (697), Expect = 0.0 Identities = 697/697 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 Score = 471 bits (245), Expect = e-129 Identities = 245/245 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIV(c) 
               
               
                   
               
               
                 Peptide sequences of protein coded by 158P3D2 v.15 
               
               
                 (SEQ ID NO: 349) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 MALTVSVQRL TGLTGTHDRQ VKLTFRGFTQ KTRKIHCGPE ADIGELFRWP HYGAPLAGEC 
                 60 
               
               
                   
               
               
                 LSVQVVNCSR VFSLRPLGTL VISLQQLQNA GHLVLREALV DENLQVSPIQ VELDLKYQPP 
                 120 
               
               
                   
               
               
                 EGATGAWSEE DFGAPIQDSF ELIIPNVGFQ ELEPGEAQLE RRAVALGRRL ARSLGQQDDE 
                 180 
               
               
                   
               
               
                 ENELELELEQ DLDDEPDVEL SGVMFSPLKS RARALAHGDP FQVSRAQDFQ VGVTVLEAQK 
                 240 
               
               
                   
               
               
                 LVGVNINPYV AVQVGGQRRV TATQRGTSCP FYNEYFLFEF HDTRLRLQDL LLEITVSGVG 
                 300 
               
               
                   
               
               
                 VTSVLQRRGD EKAAGLTPPS PKAFHSQTLP FMATRIGTFR MDLGIILDQP DGQFYQRWVP 
                 360 
               
               
                   
               
               
                 LHDPRDTRAG TKGFIKVTLS VRARGDLPPP MLPPAPGHCS DIEKNLLLPR GVPAERPWAR 
                 420 
               
               
                   
               
               
                 LRVRLYRAEG LPALRLGLLG SLVRALHDQR VLVEPYVRVS FLGQEGETSV SAEAAAPEWN 
                 480 
               
               
                   
               
               
                 EQLSFVELFP PLTRSLRLQL RDDAPLVDAA LATHVPDLRR ISHPGRAAGF NPTFGPAWVP 
                 540 
               
               
                   
               
               
                 LYGSPPGAGL RDSLQGLNEG VGQGIWFRGR LLLAVSMQVL EGRAEPEPPQ AQQGSTLSRL 
                 600 
               
               
                   
               
               
                 TRKKKKKARR DQTPKAVPQH LDASPGAEGP EIPRAMEVEV EELLPLPENV LAPCEDFLLF 
                 660 
               
               
                   
               
               
                 GVLFEATMID PTVASQPISF EISIGRAGRL EEQLGRGSRA GEGTEGAAVE AQPLLGARPE 
                 720 
               
               
                   
               
               
                 EEKEEEELGT HAQRPEPMDG SGPYFCLPLC HCKPCMHVWS CWEDHTWRLQ SSNCVRKVAE 
                 780 
               
               
                   
               
               
                 RLDQGLQEVE RLQRKPGPGA CAQLKQALEV LVAGSRQFCH GAERRTMTRP NALDRCRGKL 
                 840 
               
               
                   
               
               
                 LVHSLNLLAK QGLRLLRGLR RRNVQKKVAL AKKLLAKLRF LAEEHNFDED EMDDPGDSDG 
                 900 
               
               
                   
               
               
                 VNLISMVGEI QDQGEAEVKG TVSPKKAVAT LKIYNRSLKE EFNHFEDWLN VFPLYRGQGG 
                 960 
               
               
                   
               
               
                 QDGGGEEEGS GHLVGKFKGS FLIYPESEAV LFSEPQISRG IPQNRPIKLL VRVYVVKLRN 
                 1020 
               
               
                   
               
               
                 LCKIQGHEDF CLFSAATNLA PADPNGKADP YVVVSAGRER QDTKERYIPK QLNPIFGEIL 
                 1080 
               
               
                   
               
               
                 ELSISLPAET ELTVAVFDHD LVGSDDLIGE THIDLENRFY SHHRANCGLA SQYEVWVQQG 
                 1140 
               
               
                   
               
               
                 PQEPF 
                 1145 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LV(c) 
               
               
                   
               
               
                 Amino acid sequence alignment of 121P1F1 v.1 and 158P3D2 v.15 
               
               
                   
               
             
            
               
                 No significant similarity 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LII(d) 
               
               
                   
               
               
                 Nucleotide sequence of transcript variant 158P3D2 
               
               
                 v.16 (SEQ ID NO: 350) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 caggtgggcg ggctggtggg cagaagggca gacgggcaga ggaagtgcca gtgccactgg 
                 60 
               
               
                   
               
               
                 gaccatggct ctgacggtaa gcgtgcaacg actaacaggg ctgaccggca cccacgaccg 
                 120 
               
               
                   
               
               
                 acaagtgaag ctcacctttc gaggctttac ccagaaaaca agaaaaattc actgtggtcc 
                 180 
               
               
                   
               
               
                 agaagcagat atcggtgagc tgttccgatg gccccactat ggggctccac tggctgggga 
                 240 
               
               
                   
               
               
                 gtgtctgtct gtgcaggtgg tcaactgcag ccgtgtattc agccttaggc ctctagggac 
                 300 
               
               
                   
               
               
                 cctggtgatc tccctgcagc agctacagaa tgctgggcat ttggtgctac gggaagccct 
                 360 
               
               
                   
               
               
                 agtggatgag aatcttcaag tgtccccgat ccaggtggag cttgacctga agtaccagcc 
                 420 
               
               
                   
               
               
                 cccagagggc gctactggag cctggtcaga ggaggacttt ggggcaccca tccaggacag 
                 480 
               
               
                   
               
               
                 cttcgagtta atcatcccca atgtgggctt ccaggaactg gagcctgggg aggcccagct 
                 540 
               
               
                   
               
               
                 ggagcggcgg gcagtggctc taggccgcag gctagctcga agtctaggcc agcaggacga 
                 600 
               
               
                   
               
               
                 tgaagagaat gagctggagc ttgagctgga gcaggacctg gatgatgagc ctgacgtgga 
                 660 
               
               
                   
               
               
                 actttctggt gttatgttca gccccctcaa gagccgcgcc agggccctgg cccatgggga 
                 720 
               
               
                   
               
               
                 tcccttccag gtgtccagag ctcaagactt ccaggtggga gtcactgtgc tggaagccca 
                 780 
               
               
                   
               
               
                 gaaactggtg ggagtcaaca ttaaccccta tgtggccgtg caagtggggg ggcagcgccg 
                 840 
               
               
                   
               
               
                 tgtgaccgcc acacagcgtg ggaccagttg ccccttctac aatgagtact tcttgttcga 
                 900 
               
               
                   
               
               
                 atttcatgac acgcggcttc gtctccaaga cttgctgctg gagatcacgg tgagtggggt 
                 960 
               
               
                   
               
               
                 aggggtgacc agtgtccttc agagaagggg ggatgagaaa gctgcaggac taacaccacc 
                 1020 
               
               
                   
               
               
                 ttcccccaag gctttccatt cgcagaccct cccctttatg gccacccgga taggcacctt 
                 1080 
               
               
                   
               
               
                 caggatggac ctgggcatca tcttggacca gccagatggc cagttctacc aaagatgggt 
                 1140 
               
               
                   
               
               
                 tccgctgcat gatccccgag acacccgcgc cgggaccaag ggtttcatta aggtcacctt 
                 1200 
               
               
                   
               
               
                 gtccgtgagg gcgcgcgggg acctgccccc tccaatgcta cccccggccc cagggcactg 
                 1260 
               
               
                   
               
               
                 ttcggacatc gagaagaacc tgctcctgcc gcgcggggtg cccgccgaga ggccatgggc 
                 1320 
               
               
                   
               
               
                 gcggctccgc gtgcgcctgt accgcgccga ggggcttccc gcgctgcgcc tggggctgct 
                 1380 
               
               
                   
               
               
                 gggcagcctg gtccgcgccc tgcacgacca gcgcgtcctg gtggagccct atgtgcgggt 
                 1440 
               
               
                   
               
               
                 gtctttcctg gggcaggagg gcgagacgtc ggtgagcgcc gaggcggcgg cgcccgaatg 
                 1500 
               
               
                   
               
               
                 gaacgagcag ctgagcttcg tggagctctt cccgccgctg acgcgcagcc tccgcctgca 
                 1560 
               
               
                   
               
               
                 gctgcgggac gacgcgcccc tggtcgacgc ggcactcgct acgcacgtgc cggacctgag 
                 1620 
               
               
                   
               
               
                 gcggatctcc catccgggcc gcgcggcggg gtttaaccct accttcggcc cggcctgggt 
                 1680 
               
               
                   
               
               
                 gcccctctat ggctcgcccc ccggcgcggg gctccgggat agtcttcaag gtctcaacga 
                 1740 
               
               
                   
               
               
                 aggcgttggc caaggcattt ggttccgcgg ccgccttctg ctggctgtgt ccatgcaggt 
                 1800 
               
               
                   
               
               
                 gttggaaggg agagctgaac ctgagcctcc ccaggcccag caggggtcca cgttgtcccg 
                 1860 
               
               
                   
               
               
                 gctcacccga aagaagaaaa agaaagccag aagggatcag accccaaagg cggttccgca 
                 1920 
               
               
                   
               
               
                 gcacttggac gccagccccg gtgccgaggg gcctgagatc ccccgtgcca tggaggtgga 
                 1980 
               
               
                   
               
               
                 ggtggaggag ctgctgccgc tgccagagaa tgtcctggcg ccctgtgaag atttcctgct 
                 2040 
               
               
                   
               
               
                 tttcggtgtg ctcttcgagg ccaccatgat cgaccccacc gtggcctccc agcccatcag 
                 2100 
               
               
                   
               
               
                 cttcgagatc tccattggtc gcgcaggccg tctggaggag caattgggcc gagggtccag 
                 2160 
               
               
                   
               
               
                 ggctggggag ggaactgagg gtgcagccgt ggaggctcag cctctgctgg gagccaggcc 
                 2220 
               
               
                   
               
               
                 agaggaggag aaagaggagg aagaactggg gacccatgct cagcggcctg agcccatgga 
                 2280 
               
               
                   
               
               
                 cggcagtggg ccatacttct gcttgcccct ctgtcactgc aagccatgca tgcatgtgtg 
                 2340 
               
               
                   
               
               
                 gagttgctgg gaggaccaca cctggcgcct gcagagcagc aactgcgtgc gcaaagtggc 
                 2400 
               
               
                   
               
               
                 cgagaggctg gaccaggggc tgcaggaggt tgagagactg cagcgcaagc cggggcctgg 
                 2460 
               
               
                   
               
               
                 cgcctgtgca cagctcaagc aggcactgga agtactggtg gctgggagca gacagttttg 
                 2520 
               
               
                   
               
               
                 ccacggtgcc gagcgcagga cgatgacccg gcccaatgcc ctggatcgat gccgagggaa 
                 2580 
               
               
                   
               
               
                 actcctggtg cacagcctga accttttggc taagcaagga ctgcgacttc tacgcggcct 
                 2640 
               
               
                   
               
               
                 gagacggcgc aatgtgcaaa agaaggtggc actggccaag aagctcctgg caaaactgcg 
                 2700 
               
               
                   
               
               
                 ctttctggct gaggagcccc agccacccct ccccgatgtg ctggtctgga tgctcagcgg 
                 2760 
               
               
                   
               
               
                 gcagcgccgt gtggcctggg cccggatccc tgcccaggat gtgctgttct ctgtggttga 
                 2820 
               
               
                   
               
               
                 ggaggaacgg ggccgagact gtgggaagat ccagagtcta atgctcacgg cacccggggc 
                 2880 
               
               
                   
               
               
                 agcccctggt gaggtctgtg ccaagctgga gctcttcctg cggctgggcc tgggcaagca 
                 2940 
               
               
                   
               
               
                 agccaaggcc tgcacctctg agctgccccc ggatttgctg cccgagccct cagccgggct 
                 3000 
               
               
                   
               
               
                 gccctccagc ctacaccggg acgactttag ctacttccaa ctccgggctc acttgtacca 
                 3060 
               
               
                   
               
               
                 ggcccggggt gtgttggctg cagatgacag tggcctctcg gacccctttg ctcgagtcct 
                 3120 
               
               
                   
               
               
                 catctctacc cagtgtcaga ccacacgggt cctggagcag acgctgagcc ctctgtggga 
                 3180 
               
               
                   
               
               
                 tgaactcctg gtatttgagc agttgatcgt ggatgggagg agggagcacc tgcaggagga 
                 3240 
               
               
                   
               
               
                 gcctccatta gtgatcatca atgtatttga ccacaataag tttggccccc ccgtgttcct 
                 3300 
               
               
                   
               
               
                 gggcagggca ctggccgccc caagggtaaa gctgatggag gacccatacc aacgcccaga 
                 3360 
               
               
                   
               
               
                 gttgcagttc ttccccctga ggaagggacc ctgggcagcc ggagagctca ttgccgcctt 
                 3420 
               
               
                   
               
               
                 tcaactcatt gaactagact acagtggccg acttgagccc tcagtgccca gtgaggtgga 
                 3480 
               
               
                   
               
               
                 gccccaggat ctggcacccc tggttgagcc ccactctgga cgcctgtccc ttccacccaa 
                 3540 
               
               
                   
               
               
                 cgtgtgccca gtgctcaggg agttccgtgt tgaggtgctg ttctggggtc ttaggggact 
                 3600 
               
               
                   
               
               
                 tggtcgtgtg catctgctcg aggtggagca gccccaggtt gtactggagg tggctgggca 
                 3660 
               
               
                   
               
               
                 aggtgtggag tctgaggtcc tggccagcta ccgtgagagc cccaatttca ctgagcttgt 
                 3720 
               
               
                   
               
               
                 caggcatctg acagtggtct tcaaagacac agctcctctc ttccaccccc aggacttgcc 
                 3780 
               
               
                   
               
               
                 ggagcagcct tacttgcagc ctccactcag catcttggtg attgagcgcc gggcctttgg 
                 3840 
               
               
                   
               
               
                 ccacacagtc cttgtgggtt cccacattgt cccccacatg ctgcgattca catttcgggg 
                 3900 
               
               
                   
               
               
                 tcatgaggat cctcctgagg aggaaggaga gatggaggag acaggggata tgatgcccaa 
                 3960 
               
               
                   
               
               
                 gggacctcaa ggacagaagt ccctggatcc cttcttggct gaagcgggta tatccagaca 
                 4020 
               
               
                   
               
               
                 gctcctgaag cctcctctga agaagctccc actaggaggc ctcctaaatc aaggccctgg 
                 4080 
               
               
                   
               
               
                 gctggaggaa gacatcccag atccagagga gctcgactgg gggtccaagt actatgcgtc 
                 4140 
               
               
                   
               
               
                 gctgcaggag ctccaggggc agcacaactt tgatgaagat gaaatggatg atcctggaga 
                 4200 
               
               
                   
               
               
                 ttcagatggg gtcaacctca tttctatggt tggggagatc caagaccagg gtgaggctga 
                 4260 
               
               
                   
               
               
                 agtcaaaggc actgtgtccc caaaaaaagc agttgccacc ctgaagatct acaacaggtc 
                 4320 
               
               
                   
               
               
                 cctgaaggaa gaatttaacc actttgaaga ctggctgaat gtgtttcctc tgtaccgagg 
                 4380 
               
               
                   
               
               
                 gcaagggggc caggatggag gtggagaaga ggaaggatct ggacaccttg tgggcaagtt 
                 4440 
               
               
                   
               
               
                 caagggctcc ttcctcattt accctgaatc agaggcagtg ttgttctctg agccccagat 
                 4500 
               
               
                   
               
               
                 ctcccggggg atcccacaga accggcccat caagctcctg gtcagagtgt atgttgtaaa 
                 4560 
               
               
                   
               
               
                 ggctaccaac ctggctcctg cagaccccaa tggcaaagca gacccttacg tggtggtgag 
                 4620 
               
               
                   
               
               
                 cgctggccgg gagcggcagg acaccaagga acgctacatc cccaagcagc tcaaccccat 
                 4680 
               
               
                   
               
               
                 ctttggagag atcctggagc taagcatctc tctcccagct gagacggagc tgacggtcgc 
                 4740 
               
               
                   
               
               
                 cgtatttgat catgacctcg tgggttctga cgacctcatc ggggagaccc acattgatct 
                 4800 
               
               
                   
               
               
                 ggaaaaccga ttctatagcc accacagagc aaactgtggg ctggcctccc agtatgaagt 
                 4860 
               
               
                   
               
               
                 agatggttac aatgcctggc gtgatgcatt ctggccttcg cagatcctgg cggggctgtg 
                 4920 
               
               
                   
               
               
                 ccaacgctgt ggcctccctg cccctgaata ccgagccggt gctgtcaagg tgggcagcaa 
                 4980 
               
               
                   
               
               
                 agtcttcctg acaccaccgg agaccctgcc cccagtggcg agcggggacc ctgaagaggc 
                 5040 
               
               
                   
               
               
                 ccaggcattg cttgtgctgc ggcgctggca ggaaatgccg ggttttggga tccagctggt 
                 5100 
               
               
                   
               
               
                 acccgagcat gtagaaacca ggcctctcta ccatccccac agcccagggc tgctacaggg 
                 5160 
               
               
                   
               
               
                 atctcttcac atgtggattg acatctttcc tcaagatgtg cctgctccac ccccagttga 
                 5220 
               
               
                   
               
               
                 catcaagcct cggcagccaa tcagctatga gctcagagtt gtcatctgga acacggagga 
                 5280 
               
               
                   
               
               
                 tgtggttctg gatgacgaga atccactcac cggagagatg tcgagtgaca tctatgtgaa 
                 5340 
               
               
                   
               
               
                 gagctgggtg aaggggttgg agcatgacaa gcaggagaca gacgttcact tcaactccct 
                 5400 
               
               
                   
               
               
                 gactggggag gggaacttca attggcgctt tgtgttccgc tttgactacc tgcccacgga 
                 5460 
               
               
                   
               
               
                 gcgggaggtg agcgtctggc gcaggtctgg accctttgcc ctggaggagg cggagttccg 
                 5520 
               
               
                   
               
               
                 gcagcctgca gtgctggtcc tgcaggtctg ggactatgac cgcatctctg ccaatgactt 
                 5580 
               
               
                   
               
               
                 ccttggatcc ctggagttgc agctaccaga catggtgcgt ggggcccggg gccccgagct 
                 5640 
               
               
                   
               
               
                 ctgctctgtg cagctggccc gcaatggggc cgggccgagg tgcaatctgt ttcgctgccg 
                 5700 
               
               
                   
               
               
                 ccgcctgagg ggctggtggc cggtagtgaa gctgaaggag gcagaggacg gcaaggtgga 
                 5760 
               
               
                   
               
               
                 ggcagagttt gagctgctga ctgtggagga ggccgagaaa cggccagtgg ggaaggggcg 
                 5820 
               
               
                   
               
               
                 gaagcagcca gagcctctgg agaaacccag ccgccccaaa acttccttca actggtttgt 
                 5880 
               
               
                   
               
               
                 gaacccgctg aagacctttg tcttcttcat ctggcgccgg tactggcgca ccctggtgct 
                 5940 
               
               
                   
               
               
                 gctgctactg gtgctgctca ccgtcttcct cctcctggtc ttctacacca tccctggcca 
                 6000 
               
               
                   
               
               
                 gatcagccag gtcatcttcc gtcccctcca caagtgactc tcgctgacct tggacactca 
                 6060 
               
               
                   
               
               
                 cccagggtgc caacccttca atgcctgctc ctggaagtct ttcttaccca tgtgagctac 
                 6120 
               
               
                   
               
               
                 cccagagtct agtgcttcct ctgaataaac ctatcacagc c 
                 6161 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIII(d) 
               
               
                   
               
               
                 Nucleotide sequence alignment of 121P1F1 v.1 (SEQ ID NO: 351) and 
               
               
                  158P3D2 v.16 (SEQ ID NO: 352) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Score = 1144 bits (595), Expect = 0.0Identities = 595/595 (100%) 
               
               
                 Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Score = 792 bits (412), Expect = 0.0Identities = 412/412 (100%) 
               
               
                 Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 Score = 298 bits (155), Expect = 3e−77Identities = 155/155 
               
               
                 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIV(d) 
               
               
                   
               
               
                 Peptide sequences of protein coded by 158P3D2 v.16 (SEQ 
               
               
                 ID NO: 353) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 MALTVSVQRL TGLTGTHDRQ VKLTFRGFTQ KTRKIHCGPE ADIGELFRWP HYGAPLAGEC 
                 60 
               
               
                   
               
               
                 LSVQVVNCSR VFSLRPLGTL VISLQQLQNA GHLVLREALV DENLQVSPIQ VELDLKYQPP 
                 120 
               
               
                   
               
               
                 EGATGAWSEE DFGAPIQDSF ELIIPNVGFQ ELEPGEAQLE RRAVALGRRL ARSLGQQDDE 
                 180 
               
               
                   
               
               
                 ENELELELEQ DLDDEPDVEL SGVMFSPLKS RARALAHGDP FQVSRAQDFQ VGVTVLEAQK 
                 240 
               
               
                   
               
               
                 LVGVNINPYV AVQVGGQRRV TATQRGTSCP FYNEYFLFEF HDTRLRLQDL LLEITVSGVG 
                 300 
               
               
                   
               
               
                 VTSVLQRRGD EKAAGLTPPS PKAFHSQTLP FMATRIGTFR MDLGIILDQP DGQFYQRWVP 
                 360 
               
               
                   
               
               
                 LHDPRDTRAG TKGFIKVTLS VRARGDLPPP MLPPAPGHCS DIEKNLLLPR GVPAERPWAR 
                 420 
               
               
                   
               
               
                 LRVRLYRAEG LPALRLGLLG SLVRALHDQR VLVEPYVRVS FLGQEGETSV SAEAAAPEWN 
                 480 
               
               
                   
               
               
                 EQLSFVELFP PLTRSLRLQL RDDAPLVDAA LATHVPDLRR ISHPGRAAGF NPTFGPAWVP 
                 540 
               
               
                   
               
               
                 LYGSPPGAGL RDSLQGLNEG VGQGIWFRGR LLLAVSMQVL EGRAEPEPPQ AQQGSTLSRL 
                 600 
               
               
                   
               
               
                 TRKKKKKARR DQTPKAVPQH LDASPGAEGP EIPRAMEVEV EELLPLPENV LAPCEDFLLF 
                 660 
               
               
                   
               
               
                 GVLFEATMID PTVASQPISF EISIGRAGRL EEQLGRGSRA GEGTEGAAVE AQPLLGARPE 
                 720 
               
               
                   
               
               
                 EEKEEEELGT HAQRPEPMDG SGPYFCLPLC HCKPCMHVWS CWEDHTWRLQ SSNCVRKVAE 
                 780 
               
               
                   
               
               
                 RLDQGLQEVE RLQRKPGPGA CAQLKQALEV LVAGSRQFCH GAERRTMTRP NALDRCRGKL 
                 840 
               
               
                   
               
               
                 LVHSLNLLAK QGLRLLRGLR RRNVQKKVAL AKKLLAKLRF LAEEPQPPLP DVLVWMLSGQ 
                 900 
               
               
                   
               
               
                 RRVAWARIPA QDVLFSVVEE ERGRDCGKIQ SLMLTAPGAA PGEVCAKLEL FLRLGLGKQA 
                 960 
               
               
                   
               
               
                 KACTSELPPD LLPEPSAGLP SSLHRDDFSY FQLRAHLYQA RGVLAADDSG LSDPFARVLI 
                 1020 
               
               
                   
               
               
                 STQCQTTRVL EQTLSPLWDE LLVFEQLIVD GRREHLQEEP PLVIINVFDH NKFGPPVFLG 
                 1080 
               
               
                   
               
               
                 RALAAPRVKL MEDPYQRPEL QFFPLRKGPW AAGELIAAFQ LIELDYSGRL EPSVPSEVEP 
                 1140 
               
               
                   
               
               
                 QDLAPLVEPH SGRLSLPPNV CPVLREFRVE VLFWGLRGLG RVHLLEVEQP QVVLEVAGQG 
                 1200 
               
               
                   
               
               
                 VESEVLASYR ESPNFTELVR HLTVVFKDTA PLFHPQDLPE QPYLQPPLSI LVIERRAFGH 
                 1260 
               
               
                   
               
               
                 TVLVGSHIVP HMLRFTFRGH EDPPEEEGEM EETGDMMPKG PQGQKSLDPF LAEAGISRQL 
                 1320 
               
               
                   
               
               
                 LKPPLKKLPL GGLLNQGPGL EEDIPDPEEL DWGSKYYASL QELQGQHNFD EDEMDDPGDS 
                 1380 
               
               
                   
               
               
                 DGVNLISMVG EIQDQGEAEV KGTVSPKKAV ATLKIYNRSL KEEFNHFEDW LNVFPLYRGQ 
                 1440 
               
               
                   
               
               
                 GGQDGGGEEE GSGHLVGKFK GSFLIYPESE AVLFSEPQIS RGIPQNRPIK LLVRVYVVKA 
                 1500 
               
               
                   
               
               
                 TNLAPADPNG KADPYVVVSA GRERQDTKER YIPKQLNPIF GEILELSISL PAETELTVAV 
                 1560 
               
               
                   
               
               
                 FDHDLVGSDD LIGETHIDLE NRFYSHHRAN CGLASQYEVD GYNAWRDAFW PSQILAGLCQ 
                 1620 
               
               
                   
               
               
                 RCGLPAPEYR AGAVKVGSKV FLTPPETLPP VASGDPEEAQ ALLVLRRWQE MPGFGIQLVP 
                 1680 
               
               
                   
               
               
                 EHVETRPLYH PHSPGLLQGS LHMWIDIFPQ DVPAPPPVDI KPRQPISYEL RVVIWNTEDV 
                 1740 
               
               
                   
               
               
                 VLDDENPLTG EMSSDIYVKS WVKGLEHDKQ ETDVHFNSLT GEGNFNWRFV FRFDYLPTER 
                 1800 
               
               
                   
               
               
                 EVSVWRRSGP FALEEAEFRQ PAVLVLQVWD YDRISANDFL GSLELQLPDM VRGARGPELC 
                 1860 
               
               
                   
               
               
                 SVQLARNGAG PRCNLFRCRR LRGWWPVVKL KEAEDGKVEA EFELLTVEEA EKRPVGKGRK 
                 1920 
               
               
                   
               
               
                 QPEPLEKPSR PKTSFNWFVN PLKTFVFFIW RRYWRTLVLL LLVLLTVFLL LVFYTIPGQI 
                 1980 
               
               
                   
               
               
                 SQVIFRPLHK 
                 1990 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LV(d) 
               
               
                   
               
               
                 Amino acid sequence alignment of 121P1F1 v.1 (SEQ ID 
               
               
                 NO: 354) and 158P3D2 v.16 (SEQ ID NO: 355) 
               
               
                 Score = 580 bits (1496), Expect = e-164Identities = 288/328 
               
               
                 (87%), Positives = 288/328 (87%), Gaps = 40/328 (12%) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 V.1: 
                 1 
                 MWIDIFPQDVPAPPPVDIKPRQPISYELRVVIWNTEDVVLDDENPLTGEMSSDIYVKSWV 
                 60 
               
               
                   
                   
                 MWIDIFPQDVPAPPPVDIKPRQPISYELRVVIWNTEDVVLDDENPLTGEMSSDIYVKSWV 
                   
               
               
                 V.16: 
                 1703 
                 MWIDIFPQDVPAPPPVDIKPRQPISYELRVVIWNTEDVVLDDENPLTGEMSSDIYVKSWV 
                 1762 
               
               
                   
               
               
                 V.1: 
                 61 
                 KGLEHDKQETDVHFNSLTGEGNFNWRFVFRFDYLPTEREVSVWRRSGPFALEEAEFRQPA 
                 120 
               
               
                   
                   
                 KGLEHDKQETDVHFNSLTGEGNFNWRFVFRFDYLPTEREVSVWRRSGPFALEEAEFRQPA 
                   
               
               
                 V.16: 
                 1763 
                 KGLEHDKQETDVHFNSLTGEGNFNWRFVFRFDYLPTEREVSVWRRSGPFALEEAEFRQPA 
                 1822 
               
               
                   
               
               
                 V.1: 
                 121 
                 VLVLQVWDYDRISANDFLGSLELQLPDMVRGARGPELCSVQLARNGAGPRCNLFRCRRLR 
                 180 
               
               
                   
                   
                 VLVLQVWDYDRISANDFLGSLELQLPDMVRGARGPELCSVQLARNGAGPRCNLFRCRRLR 
                   
               
               
                 V.16: 
                 1823 
                 VLVLQVWDYDRISANDFLGSLELQLPDMVRGARGPELCSVQLARNGAGPRCNLFRCRRLR 
                 1882 
               
               
                   
               
               
                 V.1: 
                 181 
                 GWWPVVKLKEAEDVEREAQEAQAGKKKRKQRRRKGRPEDLEFTDMGGNVYILTGKVEAEF 
                 240 
               
               
                   
                   
                 GWWPVVKLKEAED                                        GKVEAEF 
                   
               
               
                 V.16 
                 1883 
                 GWWPVVKLKEAED----------------------------------------GKVEAEF 
                 1902 
               
               
                   
               
               
                 V.1: 
                 241 
                 ELLTVEEAEKRPVGKGRKQPEPLEKPSRPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLL 
                 300 
               
               
                   
                   
                 ELLTVEEAEKRPVGKGRKQPEPLEKPSRPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLL 
                   
               
               
                 V.16: 
                 1903 
                 ELLTVEEAEKRPVGKGRKQPEPLEKPSRPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLL 
                 1962 
               
               
                   
               
               
                 V.1: 
                 301 
                 VLLTVFLLLVFYTIPGQISQVIFRPLHK 
                 328 
               
               
                   
                   
                 VLLTVFLLLVFYTIPGQISQVIFRPLHK 
                   
               
               
                 V.16: 
                 1963 
                 VLLTVFLLLVFYTIPGQISQVIFRPLHK 
                 1990 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LII(e) 
               
               
                   
               
               
                 Nucleotide sequence of transcript variant 158P3D2 
               
               
                 v.17 (SEQ ID NO: 356) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 caggtgggcg ggctggtggg cagaagggca gacgggcaga ggaagtgcca gtgccactgg 
                 60 
               
               
                   
               
               
                 gaccatggct ctgacggtaa gcgtgcaacg actaacaggg ctgaccggca cccacgaccg 
                 120 
               
               
                   
               
               
                 acaagtgaag ctcacctttc gaggctttac ccagaaaaca agaaaaattc actgtggtcc 
                 180 
               
               
                   
               
               
                 agaagcagat atcggtgagc tgttccgatg gccccactat ggggctccac tggctgggga 
                 240 
               
               
                   
               
               
                 gtgtctgtct gtgcaggtgg tcaactgcag ccgtgtattc agccttaggc ctctagggac 
                 300 
               
               
                   
               
               
                 cctggtgatc tccctgcagc agctacagaa tgctgggcat ttggtgctac gggaagccct 
                 360 
               
               
                   
               
               
                 agtggatgag aatcttcaag tgtccccgat ccaggtggag cttgacctga agtaccagcc 
                 420 
               
               
                   
               
               
                 cccagagggc gctactggag cctggtcaga ggaggacttt ggggcaccca tccaggacag 
                 480 
               
               
                   
               
               
                 cttcgagtta atcatcccca atgtgggctt ccaggaactg gagcctgggg aggcccagct 
                 540 
               
               
                   
               
               
                 ggagcggcgg gcagtggctc taggccgcag gctagctcga agtctaggcc agcaggacga 
                 600 
               
               
                   
               
               
                 tgaagagaat gagctggagc ttgagctgga gcaggacctg gatgatgagc ctgacgtgga 
                 660 
               
               
                   
               
               
                 actttctggt gttatgttca gccccctcaa gagccgcgcc agggccctgg cccatgggga 
                 720 
               
               
                   
               
               
                 tcccttccag gtgtccagag ctcaagactt ccaggtggga gtcactgtgc tggaagccca 
                 780 
               
               
                   
               
               
                 gaaactggtg ggagtcaaca ttaaccccta tgtggccgtg caagtggggg ggcagcgccg 
                 840 
               
               
                   
               
               
                 tgtgaccgcc acacagcgtg ggaccagttg ccccttctac aatgagtact tcttgttcga 
                 900 
               
               
                   
               
               
                 atttcatgac acgcggcttc gtctccaaga cttgctgctg gagatcacgg tgagtggggt 
                 960 
               
               
                   
               
               
                 aggggtgacc agtgtccttc agagaagggg ggatgagaaa gctgcaggac taacaccacc 
                 1020 
               
               
                   
               
               
                 ttcccccaag gctttccatt cgcagaccct cccctttatg gccacccgga taggcacctt 
                 1080 
               
               
                   
               
               
                 caggatggac ctgggcatca tcttggacca gccagatggc cagttctacc aaagatgggt 
                 1140 
               
               
                   
               
               
                 tccgctgcat gatccccgag acacccgcgc cgggaccaag ggtttcatta aggtcacctt 
                 1200 
               
               
                   
               
               
                 gtccgtgagg gcgcgcgggg acctgccccc tccaatgcta cccccggccc cagggcactg 
                 1260 
               
               
                   
               
               
                 ttcggacatc gagaagaacc tgctcctgcc gcgcggggtg cccgccgaga ggccatgggc 
                 1320 
               
               
                   
               
               
                 gcggctccgc gtgcgcctgt accgcgccga ggggcttccc gcgctgcgcc tggggctgct 
                 1380 
               
               
                   
               
               
                 gggcagcctg gtccgcgccc tgcacgacca gcgcgtcctg gtggagccct atgtgcgggt 
                 1440 
               
               
                   
               
               
                 gtctttcctg gggcaggagg gcgagacgtc ggtgagcgcc gaggcggcgg cgcccgaatg 
                 1500 
               
               
                   
               
               
                 gaacgagcag ctgagcttcg tggagctctt cccgccgctg acgcgcagcc tccgcctgca 
                 1560 
               
               
                   
               
               
                 gctgcgggac gacgcgcccc tggtcgacgc ggcactcgct acgcacgtgc cggacctgag 
                 1620 
               
               
                   
               
               
                 gcggatctcc catccgggcc gcgcggcggg gtttaaccct accttcggcc cggcctgggt 
                 1680 
               
               
                   
               
               
                 gcccctctat ggctcgcccc ccggcgcggg gctccgggat agtcttcaag gtctcaacga 
                 1740 
               
               
                   
               
               
                 aggcgttggc caaggcattt ggttccgcgg ccgccttctg ctggctgtgt ccatgcaggt 
                 1800 
               
               
                   
               
               
                 gttggaaggg agagctgaac ctgagcctcc ccaggcccag caggggtcca cgttgtcccg 
                 1860 
               
               
                   
               
               
                 gctcacccga aagaagaaaa agaaagccag aagggatcag accccaaagg cggttccgca 
                 1920 
               
               
                   
               
               
                 gcacttggac gccagccccg gtgccgaggg gcctgagatc ccccgtgcca tggaggtgga 
                 1980 
               
               
                   
               
               
                 ggtggaggag ctgctgccgc tgccagagaa tgtcctggcg ccctgtgaag atttcctgct 
                 2040 
               
               
                   
               
               
                 tttcggtgtg ctcttcgagg ccaccatgat cgaccccacc gtggcctccc agcccatcag 
                 2100 
               
               
                   
               
               
                 cttcgagatc tccattggtc gcgcaggccg tctggaggag caattgggcc gagggtccag 
                 2160 
               
               
                   
               
               
                 ggctggggag ggaactgagg gtgcagccgt ggaggctcag cctctgctgg gagccaggcc 
                 2220 
               
               
                   
               
               
                 agaggaggag aaagaggagg aagaactggg gacccatgct cagcggcctg agcccatgga 
                 2280 
               
               
                   
               
               
                 cggcagtggg ccatacttct gcttgcccct ctgtcactgc aagccatgca tgcatgtgtg 
                 2340 
               
               
                   
               
               
                 gagttgctgg gaggaccaca cctggcgcct gcagagcagc aactgcgtgc gcaaagtggc 
                 2400 
               
               
                   
               
               
                 cgagaggctg gaccaggggc tgcaggaggt tgagagactg cagcgcaagc cggggcctgg 
                 2460 
               
               
                   
               
               
                 cgcctgtgca cagctcaagc aggcactgga agtactggtg gctgggagca gacagttttg 
                 2520 
               
               
                   
               
               
                 ccacggtgcc gagcgcagga cgatgacccg gcccaatgcc ctggatcgat gccgagggaa 
                 2580 
               
               
                   
               
               
                 actcctggtg cacagcctga accttttggc taagcaagga ctgcgacttc tacgcggcct 
                 2640 
               
               
                   
               
               
                 gagacggcgc aatgtgcaaa agaaggtggc actggccaag aagctcctgg caaaactgcg 
                 2700 
               
               
                   
               
               
                 ctttctggct gaggagcccc agccacccct ccccgatgtg ctggtctgga tgctcagcgg 
                 2760 
               
               
                   
               
               
                 gcagcgccgt gtggcctggg cccggatccc tgcccaggat gtgctgttct ctgtggttga 
                 2820 
               
               
                   
               
               
                 ggaggaacgg ggccgagact gtgggaagat ccagagtcta atgctcacgg cacccggggc 
                 2880 
               
               
                   
               
               
                 agcccctggt gaggtctgtg ccaagctgga gctcttcctg cggctgggcc tgggcaagca 
                 2940 
               
               
                   
               
               
                 agccaaggcc tgcacctctg agctgccccc ggatttgctg cccgagccct cagccgggct 
                 3000 
               
               
                   
               
               
                 gccctccagc ctacaccggg acgactttag ctacttccaa ctccgggctc acttgtacca 
                 3060 
               
               
                   
               
               
                 ggcccggggt gtgttggctg cagatgacag tggcctctcg gacccctttg ctcgagtcct 
                 3120 
               
               
                   
               
               
                 catctctacc cagtgtcaga ccacacgggt cctggagcag acgctgagcc ctctgtggga 
                 3180 
               
               
                   
               
               
                 tgaactcctg gtatttgagc agttgatcgt ggatgggagg agggagcacc tgcaggagga 
                 3240 
               
               
                   
               
               
                 gcctccatta gtgatcatca atgtatttga ccacaataag tttggccccc ccgtgttcct 
                 3300 
               
               
                   
               
               
                 gggcagggca ctggccgccc caagggtaaa gctgatggag gacccatacc aacgcccaga 
                 3360 
               
               
                   
               
               
                 gttgcagttc ttccccctga ggaagggacc ctgggcagcc ggagagctca ttgccgcctt 
                 3420 
               
               
                   
               
               
                 tcaactcatt gaactagact acagtggccg acttgagccc tcagtgccca gtgaggtgga 
                 3480 
               
               
                   
               
               
                 gccccaggat ctggcacccc tggttgagcc ccactctgga cgcctgtccc ttccacccaa 
                 3540 
               
               
                   
               
               
                 cgtgtgccca gtgctcaggg agttccgtgt tgaggtgctg ttctggggtc ttaggggact 
                 3600 
               
               
                   
               
               
                 tggtcgtgtg catctgctcg aggtggagca gccccaggtt gtactggagg tggctgggca 
                 3660 
               
               
                   
               
               
                 aggtgtggag tctgaggtcc tggccagcta ccgtgagagc cccaatttca ctgagcttgt 
                 3720 
               
               
                   
               
               
                 caggcatctg acagtggtct tcaaagacac agctcctctc ttccaccccc aggacttgcc 
                 3780 
               
               
                   
               
               
                 ggagcagcct tacttgcagc ctccactcag catcttggtg attgagcgcc gggcctttgg 
                 3840 
               
               
                   
               
               
                 ccacacagtc cttgtgggtt cccacattgt cccccacatg ctgcgattca catttcgggg 
                 3900 
               
               
                   
               
               
                 tcatgaggat cctcctgagg aggaaggaga gatggaggag acaggggata tgatgcccaa 
                 3960 
               
               
                   
               
               
                 gggacctcaa ggacagaagt ccctggatcc cttcttggct gaagcgggta tatccagaca 
                 4020 
               
               
                   
               
               
                 gctcctgaag cctcctctga agaagctccc actaggaggc ctcctaaatc aaggccctgg 
                 4080 
               
               
                   
               
               
                 gctggaggaa gacatcccag atccagagga gctcgactgg gggtccaagt actatgcgtc 
                 4140 
               
               
                   
               
               
                 gctgcaggag ctccaggggc agcacaactt tgatgaagat gaaatggatg atcctggaga 
                 4200 
               
               
                   
               
               
                 ttcagatggg gtcaacctca tttctatggt tggggagatc caagaccagg gtgaggctga 
                 4260 
               
               
                   
               
               
                 agtcaaaggc actgtgtccc caaaaaaagc agttgccacc ctgaagatct acaacaggtc 
                 4320 
               
               
                   
               
               
                 cctgaaggaa gaatttaacc actttgaaga ctggctgaat gtgtttcctc tgtaccgagg 
                 4380 
               
               
                   
               
               
                 gcaagggggc caggatggag gtggagaaga ggaaggatct ggacaccttg tgggcaagtt 
                 4440 
               
               
                   
               
               
                 caagggctcc ttcctcattt accctgaatc agaggcagtg ttgttctctg agccccagat 
                 4500 
               
               
                   
               
               
                 ctcccggggg atcccacaga accggcccat caagctcctg gtcagagtgt atgttgtaaa 
                 4560 
               
               
                   
               
               
                 ggctaccaac ctggctcctg cagaccccaa tggcaaagca gacccttacg tggtggtgag 
                 4620 
               
               
                   
               
               
                 cgctggccgg gagcggcagg acaccaagga acgctacatc cccaagcagc tcaaccccat 
                 4680 
               
               
                   
               
               
                 ctttggagag atcctggagc taagcatctc tctcccagct gagacggagc tgacggtcgc 
                 4740 
               
               
                   
               
               
                 cgtatttgat catgacctcg tgggttctga cgacctcatc ggggagaccc acattgatct 
                 4800 
               
               
                   
               
               
                 ggaaaaccga ttctatagcc accacagagc aaactgtggg ctggcctccc agtatgaagt 
                 4860 
               
               
                   
               
               
                 agatggttac aatgcctggc gtgatgcatt ctggccttcg cagatcctgg cggggctgtg 
                 4920 
               
               
                   
               
               
                 ccaacgctgt ggcctccctg cccctgaata ccgagccggt gctgtcaagg tgggcagcaa 
                 4980 
               
               
                   
               
               
                 agtcttcctg acaccaccgg agaccctgcc cccaggcagc agcagcccca cagtggcgag 
                 5040 
               
               
                   
               
               
                 cggggaccct gaagaggccc aggcattgct tgtgctgcgg cgctggcagg aaatgccggg 
                 5100 
               
               
                   
               
               
                 ttttgggatc cagctggtac ccgagcatgt agaaaccagg cctctctacc atccccacag 
                 5160 
               
               
                   
               
               
                 cccagggctg ctacagggat ctcttcacat gtggattgac atctttcctc aagatgtgcc 
                 5220 
               
               
                   
               
               
                 tgctccaccc ccagttgaca tcaagcctcg gcagccaatc agctatgagc tcagagttgt 
                 5280 
               
               
                   
               
               
                 catctggaac acggaggatg tggttctgga tgacgagaat ccactcaccg gagagatgtc 
                 5340 
               
               
                   
               
               
                 gagtgacatc tatgtgaaga gctgggtgaa ggggttggag catgacaagc aggagacaga 
                 5400 
               
               
                   
               
               
                 cgttcacttc aactccctga ctggggaggg gaacttcaat tggcgctttg tgttccgctt 
                 5460 
               
               
                   
               
               
                 tgactacctg cccacggagc gggaggtgag cgtctggcgc aggtctggac cctttgccct 
                 5520 
               
               
                   
               
               
                 ggaggaggcg gagttccggc agcctgcagt gctggtcctg caggtctggg actatgaccg 
                 5580 
               
               
                   
               
               
                 catctctgcc aatgacttcc ttggatccct ggagttgcag ctaccagaca tggtgcgtgg 
                 5640 
               
               
                   
               
               
                 ggcccggggc cccgagctct gctctgtgca gctggcccgc aatggggccg ggccgaggtg 
                 5700 
               
               
                   
               
               
                 caatctgttt cgctgccgcc gcctgagggg ctggtggccg gtagtgaagc tgaaggaggc 
                 5760 
               
               
                   
               
               
                 agaggacgtg gagcgggagg cgcaggaggc tcaggctggc aagaagaagc gaaagcagag 
                 5820 
               
               
                   
               
               
                 gaggaggaag ggccggccag aagacctgga gttcacagac atgggtggca atgtgtacat 
                 5880 
               
               
                   
               
               
                 cctcacgggc aaggtggagg cagagtttga gctgctgact gtggaggagg ccgagaaacg 
                 5940 
               
               
                   
               
               
                 gccagtgggg aaggggcgga agcagccaga gcctctggag aaacccagcc gccccaaaac 
                 6000 
               
               
                   
               
               
                 ttccttcaac tggtttgtga acccgctgaa gacctttgtc ttcttcatct ggcgccggta 
                 6060 
               
               
                   
               
               
                 ctggcgcacc ctggtgctgc tgctactggt gctgctcacc gtcttcctcc tcctggtctt 
                 6120 
               
               
                   
               
               
                 ctacaccatc cctggccaga tcagccaggt catcttccgt cccctccaca agtgactctc 
                 6180 
               
               
                   
               
               
                 gctgaccttg gacactcacc cagggtgcca acccttcaat gcctgctcct ggaagtcttt 
                 6240 
               
               
                   
               
               
                 cttacccatg tgagctaccc cagagtctag tgcttcctct gaataaacct atcacagcc 
                 6299 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIII (e)  
               
               
                   
               
               
                 Nucleotide sequence alignment of 121P1F1 v.1 (SEQ 
               
               
                 ID NO: 357) and 158P3D2 v.17 (SEQ ID NO: 358) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Score = 2165 bits (1126), Expect = 0.0 Identities = 1126/1126 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 Score = 300 bits (156), Expect = 7e−78 Identities = 156/156 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIV(e) 
               
               
                   
               
               
                 Peptide sequences of protein coded by 158P3D2 v.17 
               
               
                 (SEQ ID NO: 359) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 MALTVSVQRL TGLTGTHDRQ VKLTFRGFTQ KTRKIHCGPE ADIGELFRWP HYGAPLAGEC 
                 60 
               
               
                   
               
               
                 LSVQVVNCSR VFSLRPLGTL VISLQQLQNA GHLVLREALV DENLQVSPIQ VELDLKYQPP 
                 120 
               
               
                   
               
               
                 EGATGAWSEE DFGAPIQDSF ELIIPNVGFQ ELEPGEAQLE RRAVALGRRL ARSLGQQDDE 
                 180 
               
               
                   
               
               
                 ENELELELEQ DLDDEPDVEL SGVMFSPLKS RARALAHGDP FQVSRAQDFQ VGVTVLEAQK 
                 240 
               
               
                   
               
               
                 LVGVNINPYV AVQVGGQRRV TATQRGTSCP FYNEYFLFEF HDTRLRLQDL LLEITVSGVG 
                 300 
               
               
                   
               
               
                 VTSVLQRRGD EKAAGLTPPS PKAFHSQTLP FMATRIGTFR MDLGIILDQP DGQFYQRWVP 
                 360 
               
               
                   
               
               
                 LHDPRDTRAG TKGFIKVTLS VRARGDLPPP MLPPAPGHCS DIEKNLLLPR GVPAERPWAR 
                 420 
               
               
                   
               
               
                 LRVRLYRAEG LPALRLGLLG SLVRALHDQR VLVEPYVRVS FLGQEGETSV SAEAAAPEWN 
                 480 
               
               
                   
               
               
                 EQLSFVELFP PLTRSLRLQL RDDAPLVDAA LATHVPDLRR ISHPGRAAGF NPTFGPAWVP 
                 540 
               
               
                   
               
               
                 LYGSPPGAGL RDSLQGLNEG VGQGIWFRGR LLLAVSMQVL EGRAEPEPPQ AQQGSTLSRL 
                 600 
               
               
                   
               
               
                 TRKKKKKARR DQTPKAVPQH LDASPGAEGP EIPRAMEVEV EELLPLPENV LAPCEDFLLF 
                 660 
               
               
                   
               
               
                 GVLFEATMID PTVASQPISF EISIGRAGRL EEQLGRGSRA GEGTEGAAVE AQPLLGARPE 
                 720 
               
               
                   
               
               
                 EEKEEEELGT HAQRPEPMDG SGPYFCLPLC HCKPCMHVWS CWEDHTWRLQ SSNCVRKVAE 
                 780 
               
               
                   
               
               
                 RLDQGLQEVE RLQRKPGPGA CAQLKQALEV LVAGSRQFCH GAERRTMTRP NALDRCRGKL 
                 840 
               
               
                   
               
               
                 LVHSLNLLAK QGLRLLRGLR RRNVQKKVAL AKKLLAKLRF LAEEPQPPLP DVLVWMLSGQ 
                 900 
               
               
                   
               
               
                 RRVAWARIPA QDVLFSVVEE ERGRDCGKIQ SLMLTAPGAA PGEVCAKLEL FLRLGLGKQA 
                 960 
               
               
                   
               
               
                 KACTSELPPD LLPEPSAGLP SSLHRDDFSY FQLRAHLYQA RGVLAADDSG LSDPFARVLI 
                 1020 
               
               
                   
               
               
                 STQCQTTRVL EQTLSPLWDE LLVFEQLIVD GRREHLQEEP PLVIINVFDH NKFGPPVFLG 
                 1080 
               
               
                   
               
               
                 RALAAPRVKL MEDPYQRPEL QFFPLRKGPW AAGELIAAFQ LIELDYSGRL EPSVPSEVEP 
                 1140 
               
               
                   
               
               
                 QDLAPLVEPH SGRLSLPPNV CPVLREFRVE VLFWGLRGLG RVHLLEVEQP QVVLEVAGQG 
                 1200 
               
               
                   
               
               
                 VESEVLASYR ESPNFTELVR HLTVVFKDTA PLFHPQDLPE QPYLQPPLSI LVIERRAFGH 
                 1260 
               
               
                   
               
               
                 TVLVGSHIVP HMLRFTFRGH EDPPEEEGEM EETGDMMPKG PQGQKSLDPF LAEAGISRQL 
                 1320 
               
               
                   
               
               
                 LKPPLKKLPL GGLLNQGPGL EEDIPDPEEL DWGSKYYASL QELQGQHNFD EDEMDDPGDS 
                 1380 
               
               
                   
               
               
                 DGVNLISMVG EIQDQGEAEV KGTVSPKKAV ATLKIYNRSL KEEFNHFEDW LNVFPLYRGQ 
                 1440 
               
               
                   
               
               
                 GGQDGGGEEE GSGHLVGKFK GSFLIYPESE AVLFSEPQIS RGIPQNRPIK LLVRVYVVKA 
                 1500 
               
               
                   
               
               
                 TNLAPADPNG KADPYVVVSA GRERQDTKER YIPKQLNPIF GEILELSISL PAETELTVAV 
                 1560 
               
               
                   
               
               
                 FDHDLVGSDD LIGETHIDLE NRFYSHHRAN CGLASQYEVD GYNAWRDAFW PSQILAGLCQ 
                 1620 
               
               
                   
               
               
                 RCGLPAPEYR AGAVKVGSKV FLTPPETLPP GSSSPTVASG DPEEAQALLV LRRWQEMPGF 
                 1680 
               
               
                   
               
               
                 GIQLVPEHVE TRPLYHPHSP GLLQGSLHMW IDIFPQDVPA PPPVDIKPRQ PISYELRVVI 
                 1740 
               
               
                   
               
               
                 WNTEDVVLDD ENPLTGEMSS DIYVKSWVKG LEHDKQETDV HFNSLTGEGN FNWRFVFRFD 
                 1800 
               
               
                   
               
               
                 YLPTEREVSV WRRSGPFALE EAEFRQPAVL VLQVWDYDRI SANDFLGSLE LQLPDMVRGA 
                 1860 
               
               
                   
               
               
                 RGPELCSVQL ARNGAGPRCN LFRCRRLRGW WPVVKLKEAE DVEREAQEAQ AGKKKRKQRR 
                 1920 
               
               
                   
               
               
                 RKGRPEDLEF TDMGGNVYIL TGKVEAEFEL LTVEEAEKRP VGKGRKQPEP LEKPSRPKTS 
                 1980 
               
               
                   
               
               
                 FNWFVNPLKT FVFFIWRRYW RTLVLLLLVL LTVFLLLVFY TIPGQISQVI FRPLHK 
                 2036 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LV(e) 
               
               
                   
               
               
                 Amino acid sequence alignment of 121P1F1 v.1 (SEQ ID 
               
               
                 NO: 360) and 158P3D2 v.17 (SEQ ID NO: 361) 
               
               
                 Score = 679 bits (1751), Expect = 0.0Identities = 328/328 
               
               
                 (100%), Positives = 328/328 (100%) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 V.1: 
                 1 
                 MWIDIFPQDVPAPPPVDIKPRQPISYELRVVIWNTEDVVLDDENPLTGEMSSDIYVKSWV 
                 60 
               
               
                   
                   
                 MWIDIFPQDVPAPPPVDIKPRQPISYELRVVIWNTEDVVLDDENPLTGEMSSDIYVKSWV 
                   
               
               
                 v.17: 
                 1709 
                 MWIDIFPQDVPAPPPVDIKPRQPISYELRVVIWNTEDVVLDDENPLTGEMSSDIYVKSWV 
                 1768 
               
               
                   
               
               
                 V.1: 
                 61 
                 KGLEHDKQETDVHFNSLTGEGNFNWRFVFRFDYLPTEREVSVWRRSGPFALEEAEFRQPA 
                 120 
               
               
                   
                   
                 KGLEHDKQETDVHFNSLTGEGNFNWRFVFRFDYLPTEREVSVWRRSGPFALEEAEFRQPA 
                   
               
               
                 v.17: 
                 1769 
                 KGLEHDKQETDVHFNSLTGEGNFNWRFVFRFDYLPTEREVSVWRRSGPFALEEAEFRQPA 
                 1828 
               
               
                   
               
               
                 V.1: 
                 121 
                 VLVLQVWDYDRISANDFLGSLELQLPDMVRGARGPELCSVQLARNGAGPRCNLFRCRRLR 
                 180 
               
               
                   
                   
                 VLVLQVWDYDRISANDFLGSLELQLPDMVRGARGPELCSVQLARNGAGPRCNLFRCRRLR 
                   
               
               
                 v.17: 
                 1829 
                 VLVLQVWDYDRISANDFLGSLELQLPDMVRGARGPELCSVQLARNGAGPRCNLFRCRRLR 
                 1888 
               
               
                   
               
               
                 V.1: 
                 181 
                 GWWPVVKLKEAEDVEREAQEAQAGKKKRKQRRRKGRPEDLEFTDMGGNVYILTGKVEAEF 
                 240 
               
               
                   
                   
                 GWWPVVKLKEAEDVEREAQEAQAGKKKRKQRRRKGRPEDLEFTDMGGNVYILTGKVEAEF 
                   
               
               
                 v.17: 
                 1889 
                 GWWPVVKLKEAEDVEREAQEAQAGKKKRKQRRRKGRPEDLEFTDMGGNVYILTGKVEAEF 
                 1948 
               
               
                   
               
               
                 V.1: 
                 241 
                 ELLTVEEAEKRPVGKGRKQPEPLEKPSRPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLL 
                 300 
               
               
                   
                   
                 ELLTVEEAEKRPVGKGRKQPEPLEKPSRPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLL 
                   
               
               
                 v.17: 
                 1949 
                 ELLTVEEAEKRPVGKGRKQPEPLEKPSRPKTSFNWFVNPLKTFVFFIWRRYWRTLVLLLL 
                 2008 
               
               
                   
               
               
                 V.1: 
                 301 
                 VLLTVFLLLVFYTIPGQISQVIFRPLHK 
                 328 
               
               
                   
                   
                 VLLTVFLLLVFYTIPGQISQVIFRPLHK 
                   
               
               
                 v.17: 
                 2009 
                 VLLTVFLLLVFYTIPGQISQVIFRPLHK 
                 2036 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LII(f) 
               
               
                   
               
               
                 Nucleotide sequence of transcript variant 158P3D2 
               
               
                 v.18 (SEQ ID NO: 362) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 agaagaaagc tggtaggggc tgggagaggg taccacaggg gagtatgatc tacttggggg 
                 60 
               
               
                   
               
               
                 ccagagaagg ttccctgagg aaatagtacc tgaacttaga cttgaaggat aacagatgtt 
                 120 
               
               
                   
               
               
                 aactgggagg agagaatgtt ccaggcagag gaaaaggcat atgcaaaagt ccagcgcctt 
                 180 
               
               
                   
               
               
                 gaaggagcac agctggggtg cctggagtga gatggagctg gaaagatcca ggtggagctt 
                 240 
               
               
                   
               
               
                 gacctgaagt accagccccc agagggcgct actggagcct ggtcagagga ggactttggg 
                 300 
               
               
                   
               
               
                 gcacccatcc aggacagctt cgagttaatc atccccaatg tgggcttcca ggaactggag 
                 360 
               
               
                   
               
               
                 cctggggagg cccagctgga gcggcgggca gtggctctag gccgcaggct agctcgaagt 
                 420 
               
               
                   
               
               
                 ctaggccagc aggacgatga agagaatgag ctggagcttg agctggagca ggacctggat 
                 480 
               
               
                   
               
               
                 gatgagcctg acgtggaact ttctggtgtt atgttcagcc ccctcaagag ccgcgccagg 
                 540 
               
               
                   
               
               
                 gccctggccc atggggatcc cttccaggtg tccagagctc aagacttcca ggtgggagtc 
                 600 
               
               
                   
               
               
                 actgtgctgg aagcccagaa actggtggga gtcaacatta acccctatgt ggccgtgcaa 
                 660 
               
               
                   
               
               
                 gtgggggggc agcgccgtgt gaccgccaca cagcgtggga ccagttgccc cttctacaat 
                 720 
               
               
                   
               
               
                 gagtacttct tgttcgaatt tcatgacacg cggcttcgtc tccaagactt gctgctggag 
                 780 
               
               
                   
               
               
                 atcacggtga gtggggtagg ggtgaccagt gtccttcaga gaagggggga tgagaaagct 
                 840 
               
               
                   
               
               
                 gcaggactaa caccaccttc ccccaaggct ttccattcgc agaccctccc ctttatggcc 
                 900 
               
               
                   
               
               
                 acccggatag gcaccttcag gatggacctg ggcatcatct tggaccagcc aggtatggaa 
                 960 
               
               
                   
               
               
                 tcgtcccctt attgagactc tgcacggaca agggccctag agattgaccc tgcagtgact 
                 1020 
               
               
                   
               
               
                 ccgcatggac ccctatacac tcacttcgga gagggccatc tctggcggag gctgaactct 
                 1080 
               
               
                   
               
               
                 tggcacttcc gcccctccct gctgagccag agaagccctg gccattgtcc gtcactccga 
                 1140 
               
               
                   
               
               
                 tagcctcacg gccaccctgt gcgtcccgcc ggtcgcccct tacccctggc tcgccccttc 
                 1200 
               
               
                   
               
               
                 gcccttagat ggccagttct accaaagatg ggttccgctg catgatcccc gagacacccg 
                 1260 
               
               
                   
               
               
                 cgccgggacc aagggtttca ttaaggtcac cttgtccgtg agggcgcgcg gggacctgcc 
                 1320 
               
               
                   
               
               
                 ccctccaatg ctacccccgg ccccagggca ctgttcggac atcgagaagt gagccggggt 
                 1380 
               
               
                   
               
               
                 gaggtgggga ggaggacatg gatccggggg tggccgtggg gcgcggataa ggggaggggc 
                 1440 
               
               
                   
               
               
                 cgagatccca gtttctcccc ccccgctcgg tgccccctcc cctaggaacc tgctcctgcc 
                 1500 
               
               
                   
               
               
                 gcgcggggtg cccgccgaga ggccatgggc gcggctccgc gtgcgcctgt accgcgccga 
                 1560 
               
               
                   
               
               
                 ggggcttccc gcgctgcgcc tggggctgct gggcagcctg gtccgcgccc tgcacgacca 
                 1620 
               
               
                   
               
               
                 gcgcgtcctg gtggagccct atgtgcgggt gtctttcctg gggcaggagg gcgagacgtc 
                 1680 
               
               
                   
               
               
                 ggtgagcgcc gaggcggcgg cgcccgaatg gaacgagcag ctgagcttcg tggagctctt 
                 1740 
               
               
                   
               
               
                 cccgccgctg acgcgcagcc tccgcctgca gctgcgggac gacgcgcccc tggtcgacgc 
                 1800 
               
               
                   
               
               
                 ggcactcgct acgcacgtgc cggacctgag gcggatctcc catccgggcc gcgcggcggg 
                 1860 
               
               
                   
               
               
                 gtttaaccct accttcggcc cggcctgggt gcccctctat ggctcgcccc ccggcgcggg 
                 1920 
               
               
                   
               
               
                 gctccgggat agtcttcaag gtctcaacga aggcgttggc caaggcattt ggttccgcgg 
                 1980 
               
               
                   
               
               
                 ccgccttctg ctggctgtgt ccatgcaggt gttggaaggg agagctgaac ctgagcctcc 
                 2040 
               
               
                   
               
               
                 ccaggcccag caggggtcca cgttgtcccg gctcacccga aagaagaaaa agaaagccag 
                 2100 
               
               
                   
               
               
                 aagggatcag accccaaagg cggttccgca gcacttggac gccagccccg gtgccgaggg 
                 2160 
               
               
                   
               
               
                 gcctgagatc ccccgtgcca tggaggtgga ggtggaggag ctgctgccgc tgccagagaa 
                 2220 
               
               
                   
               
               
                 tgtcctggcg ccctgtgaag atttcctgct tttcggtgtg ctcttcgagg ccaccatgat 
                 2280 
               
               
                   
               
               
                 cgaccccacc gtggcctccc agcccatcag cttcgagatc tccattggtg tgtggcctag 
                 2340 
               
               
                   
               
               
                 ccgaacccct gagtgccatt tcagacctta gaaccctgga aggggtgttg actttcagtc 
                 2400 
               
               
                   
               
               
                 gcgcaggccg tctggaggag caattgggcc gagggtccag ggctggggag ggaactgagg 
                 2460 
               
               
                   
               
               
                 gtgcagccgt ggaggctcag cctctgctgg gagccaggcc agaggaggag aaagaggagg 
                 2520 
               
               
                   
               
               
                 aagaactggg gacccatgct cagcggcctg agcccatgga cggcagtggg ccatacttct 
                 2580 
               
               
                   
               
               
                 gcttgcccct ctgtcactgc aagccatgca tgcatgtgtg gagttgctgg gaggaccaca 
                 2640 
               
               
                   
               
               
                 cctggcgcct gcagagcagc aactgcgtgc gcaaagtggc cgagaggctg gaccaggggc 
                 2700 
               
               
                   
               
               
                 tgcaggaggt tgagagactg cagcgcaagc cggggcctgg cgcctgtgca cagctcaagc 
                 2760 
               
               
                   
               
               
                 aggcactgga agtactggtg gctgggagca gacagttttg ccacggtgcc gagcgcagga 
                 2820 
               
               
                   
               
               
                 cgatgacccg gcccaatgcc ctggatcgat gccgagggaa actcctggtg cacagcctga 
                 2880 
               
               
                   
               
               
                 accttttggc taagcaagga ctgcgacttc tacgcggcct gagacggcgc aatgtgcaaa 
                 2940 
               
               
                   
               
               
                 agaaggtggc actggccaag aagctcctgg caaaactgcg ctttctggct gaggaggcac 
                 3000 
               
               
                   
               
               
                 ccggggcagc ccctggtgag gtctgtgcca agctggagct cttcctgcgg ctgggcctgg 
                 3060 
               
               
                   
               
               
                 gcaagcaagc caaggcctgc acctctgagc tgcccccgga tttgctgccc gagccctcag 
                 3120 
               
               
                   
               
               
                 ccgggctgcc ctccagccta caccgggacg gtcctggagc agacgctgag ccctctgtgg 
                 3180 
               
               
                   
               
               
                 gatgaactcc tggtatttga gcagttgatc gtggatggga ggagggagca cctgcaggag 
                 3240 
               
               
                   
               
               
                 gagcctccat tagtgatcat caatgtattt gaccacaata agtttggccc ccccgtgttc 
                 3300 
               
               
                   
               
               
                 ctgggcaggg cactggccgc cccaagggta aagctgatgg aggacccata ccaacgccca 
                 3360 
               
               
                   
               
               
                 gagttgcagt tcttccccct gaggaaggga ccctgggcag ccggagagct cattgccgcc 
                 3420 
               
               
                   
               
               
                 tttcaactca ttgaactaga ctacagtggc cgacttgagc cctcagtgcc cagtgaggtg 
                 3480 
               
               
                   
               
               
                 gagccccagg atctggcacc cctggttgag ccccactctg gacgcctgtc ccttccaccc 
                 3540 
               
               
                   
               
               
                 aacgtgtgcc cagtgctcag ggagttccgt gttgaggtgc tgttctgggg tcttagggga 
                 3600 
               
               
                   
               
               
                 cttggtcgtg tgcatctgct cgaggtggag cagccccagg ttgtactgga ggtggctggg 
                 3660 
               
               
                   
               
               
                 caaggtgtgg agtctgaggt cctggccagc taccgtgaga gccccaattt cactgagctt 
                 3720 
               
               
                   
               
               
                 gtcaggcatc tgacagtggt cttcaaagac acagctcctc tcttccaccc ccaggacttg 
                 3780 
               
               
                   
               
               
                 ccggagcagc cttacttgca gcctccactc agcatcttgg tgattgagcg ccgggccttt 
                 3840 
               
               
                   
               
               
                 ggccacacag tccttgtggg ttcccacatt gtcccccaca tgctgcgatt cacatttcgg 
                 3900 
               
               
                   
               
               
                 ggtcatgagg atcctcctga ggaggaagga gagatggagg agacagggga tatgatgccc 
                 3960 
               
               
                   
               
               
                 aagggacctc aaggacagaa gtccctggat cccttcttgg ctgaagcggg tatatccaga 
                 4020 
               
               
                   
               
               
                 cagctcctga agcacaactt tgatgaagat gaaatggatg atcctggaga ttcagatggg 
                 4080 
               
               
                   
               
               
                 gtcaacctca tttctatggt tggggagatc caagaccagg gtgaggctga agtcaaaggc 
                 4140 
               
               
                   
               
               
                 actgtgtccc caaaaaaagc agttgccacc ctgaagatct acaacaggtc cctgaaggaa 
                 4200 
               
               
                   
               
               
                 gaatttaacc actttgaaga ctggctgaat gtgtttcctc tgtaccgagg gcaagggggc 
                 4260 
               
               
                   
               
               
                 caggatggag gtggagaaga ggaaggatct ggacaccttg tgggcaagtt caagggctcc 
                 4320 
               
               
                   
               
               
                 ttcctcattt accctgaatc agaggcagtg ttgttctctg agccccagat ctcccggggg 
                 4380 
               
               
                   
               
               
                 atcccacaga accggcccat caagctcctg gtcagagtgt atgttgtaaa ggctaccaac 
                 4440 
               
               
                   
               
               
                 ctggctcctg cagaccccaa tggcaaagca gacccttacg tggtggtgag cgctggccgg 
                 4500 
               
               
                   
               
               
                 gagcggcagg acaccaagga acgctacatc cccaagcagc tcaaccccat ctttggagag 
                 4560 
               
               
                   
               
               
                 atcctggagc taagcatctc tctcccagct gagacggagc tgacggtcgc cgtatttgat 
                 4620 
               
               
                   
               
               
                 catgacctcg tgggttctga cgacctcatc ggggagaccc acattgatct ggaaaaccga 
                 4680 
               
               
                   
               
               
                 ttctatagcc accacagagc aaactgtggg ctggcctccc agtatgaagt gtgggtccag 
                 4740 
               
               
                   
               
               
                 cagggcccac aggagccatt ctgagtttct ggccaaacac attcaagctc acattccctt 
                 4800 
               
               
                   
               
               
                 ttgtgtctcc agatcctatg atttcatgga aggggaccct cccacccacc gccactgcca 
                 4860 
               
               
                   
               
               
                 accaagacat agctcagtgg tcaagacttg ggcttgggag tcgggatcct gtaacgaatg 
                 4920 
               
               
                   
               
               
                 tcacttgacc gctttctttt tttatgaaac agtctcgctc tgtctcccag gttggagtgc 
                 4980 
               
               
                   
               
               
                 agtggcacga tctcggctga ctgcaacctc cacctcctgg gttcaagcga ttctcctgcc 
                 5040 
               
               
                   
               
               
                 tcagcctccc cagtagctgg gattacaggc gtgggccccc atgtccagct aatttttata 
                 5100 
               
               
                   
               
               
                 ttttcgctct gtctcccagg ttggagtgca gtggcacgat ctcggctgac tgcaacctcc 
                 5160 
               
               
                   
               
               
                 acctcctggg ttcaagcgat tctcctgcct cagcctcccc agtagctggg attacaggcg 
                 5220 
               
               
                   
               
               
                 tgggccccca tgtccagcta atttttatat ttttagtaga gacagggttt caccatgttg 
                 5280 
               
               
                   
               
               
                 tccaggctgg tcttgaaccc ctgacctcaa gtgatccacc cacctctgcc tcccaaagtg 
                 5340 
               
               
                   
               
               
                 ctgggattac aggtgtgagc caccatgcca ggccctctta acctcttcaa gtctgttttc 
                 5400 
               
               
                   
               
               
                 tcatctgcaa aacagaggta ataagatcag tatcttctta atggaagcac ctggactaca 
                 5460 
               
               
                   
               
               
                 tttttttcat tcattgttat cataaatgag gactaacctg tctcccgttg ggagttttga 
                 5520 
               
               
                   
               
               
                 acctagacct catgtcttca tgacgtcatc actgccccag gcccagctgt gtccctacac 
                 5580 
               
               
                   
               
               
                 cagccccagc tgacgcatct tctttttctg cctgtagaga tggttacaat gcctggcgtg 
                 5640 
               
               
                   
               
               
                 atgcattctg gccttcgcag atcctggcgg ggctgtgcca acgctgtggc ctccctgccc 
                 5700 
               
               
                   
               
               
                 ctgaataccg agccggtgct gtcaaggtgg gcagcaaagt cttcctgaca ccaccggaga 
                 5760 
               
               
                   
               
               
                 ccctgccccc agggatctct tcacatgtgg attgacatct ttcctcaaga tgtgcctgct 
                 5820 
               
               
                   
               
               
                 ccacccccag ttgacatcaa gcctcggcag ccaatcagct atgagctcag agttgtcatc 
                 5880 
               
               
                   
               
               
                 tggaacacgg aggatgtggt tctggatgac gagaatccac tcaccggaga gatgtcgagt 
                 5940 
               
               
                   
               
               
                 gacatctatg tgaagagctg ggtgaagggg ttggagcatg acaagcagga gacagacgtt 
                 6000 
               
               
                   
               
               
                 cacttcaact ccctgactgg ggaggggaac ttcaattggc gctttgtgtt ccgctttgac 
                 6060 
               
               
                   
               
               
                 tacctgccca cggagcggga ggtgagcgtc tggcgcaggt ctggaccctt tgccctggag 
                 6120 
               
               
                   
               
               
                 gaggcggagt tccggcagcc tgcagtgctg gtcctgcagg atccctggag ttgcagctac 
                 6180 
               
               
                   
               
               
                 cagacatggt gcgtggggcc cggggccccg agctctgctc tgtgcagctg gcccgcaatg 
                 6240 
               
               
                   
               
               
                 gggccgggcc gaggtgcaat ctgtttcgct gccgccgcct gaggggctgg tggccggtag 
                 6300 
               
               
                   
               
               
                 tgaagctgaa ggaggcagag gacgtggagc gggaggcgca ggaggctcag gctggcaaga 
                 6360 
               
               
                   
               
               
                 agaagcgaaa gcagaggagg aggaagggcc ggccagaaga cctggagttc acagacatgg 
                 6420 
               
               
                   
               
               
                 gtggcaatgt gtacatcctc acgggcaagg tggaggcaga gtttgagctg ctgactgtgg 
                 6480 
               
               
                   
               
               
                 aggaggccga gaaacggcca gtggggaagg ggcggaagca gccagagcct ctggagaaac 
                 6540 
               
               
                   
               
               
                 ccagccgccc caaaacttcc ttcaactggt ttgtgaaccc gctgaagacc tttgtcttct 
                 6600 
               
               
                   
               
               
                 tcatctggcg ccggtactgg cgcaccctgg tgctgctgct actggtgctg ctcaccgtct 
                 6660 
               
               
                   
               
               
                 tcctcctcct ggtcttctac accatccctg gccagatcag ccaggtcatc ttccgtcccc 
                 6720 
               
               
                   
               
               
                 tccacaagtg actctcgctg accttggaca ctcacccagg gtgccaaccc ttcaatgcct 
                 6780 
               
               
                   
               
               
                 gctcctggaa gtctttctta cccatgtgag ctaccccaga gtctagtgct tcctctgaat 
                 6840 
               
               
                   
               
               
                 aaacctatca cagcc 
                 6855 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIII (f)  
               
               
                   
               
               
                 Nucleotide sequence alignment of 121P1F1 v.1 (SEQ 
               
               
                 ID NO: 363) and 158P3D2 v.18 (SEQ ID NO: 364) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Score = 2354 bits (1224), Expect = 0.0 Identities = 1224/1224 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 Score = 1340 bits (697), Expect = 0.0 Identities = 697/697 (100%) Strand = Plus/Plus 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LIV(f) 
               
               
                   
               
               
                 Peptide sequences of protein coded by 158P3D2 v.18 
               
               
                 (SEQ ID NO: 365) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 MCKRRWHWPR SSWQNCAFWL RRHPGQPLVR SVPSWSSSCG WAWASKPRPA PLSCPRICCP 
                 60 
               
               
                   
               
               
                 SPQPGCPPAY TGTVLEQTLS PLWDELLVFE QLIVDGRREH LQEEPPLVII NVFDHNKFGP 
                 120 
               
               
                   
               
               
                 PVFLGRALAA PRVKLMEDPY QRPELQFFPL RKGPWAAGEL IAAFQLIELD YSGRLEPSVP 
                 180 
               
               
                   
               
               
                 SEVEPQDLAP LVEPHSGRLS LPPNVCPVLR EFRVEVLFWG LRGLGRVHLL EVEQPQVVLE 
                 240 
               
               
                   
               
               
                 VAGQGVESEV LASYRESPNF TELVRHLTVV FKDTAPLFHP QDLPEQPYLQ PPLSILVIER 
                 300 
               
               
                   
               
               
                 RAFGHTVLVG SHIVPHMLRF TFRGHEDPPE EEGEMEETGD MMPKGPQGQK SLDPFLAEAG 
                 360 
               
               
                   
               
               
                 ISRQLLKHNF DEDEMDDPGD SDGVNLISMV GEIQDQGEAE VKGTVSPKKA VATLKIYNRS 
                 420 
               
               
                   
               
               
                 LKEEFNHFED WLNVFPLYRG QGGQDGGGEE EGSGHLVGKF KGSFLIYPES EAVLFSEPQI 
                 480 
               
               
                   
               
               
                 SRGIPQNRPI KLLVRVYVVK ATNLAPADPN GKADPYVVVS AGRERQDTKE RYIPKQLNPI 
                 540 
               
               
                   
               
               
                 FGEILELSIS LPAETELTVA VFDHDLVGSD DLIGETHIDL ENRFYSHHRA NCGLASQYEV 
                 600 
               
               
                   
               
               
                 WVQQGPQEPF 
                 610 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE LV(f) 
               
               
                   
               
               
                 Amino acid sequence alignment of 121P1F1 v.1 and 158P3D2 v.18 
               
               
                   
               
             
            
               
                 No significant similarity.