Patent Publication Number: US-11034963-B2

Title: Allergen detection agents and assays

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application is a 35 U.S.C. § 371 U.S. National Stage Entry of International Application No. PCT/US2017/060487 filed Nov. 8, 2017 which claims priority to U.S. Provisional Application Ser. No. 62/418,984, filed on Nov. 8, 2016; U.S. Provisional Application Ser. No. 62/435,106, filed on Dec. 16, 2016; and U.S. Provisional Application Ser. No. 62/512,299, filed on May 30, 2017; the contents of each of which are incorporated herein by reference in their entirety. 
    
    
     REFERENCE TO SEQUECING LISTING 
     The present application is being filed along with a Sequence Listing in electronic format. The Sequence Listing is provided as a file entitled 20661007US371SEQLST.txt, created on May 7, 2019, which is 2,515,112 bytes in size. The information in the electronic format of the sequence listing is incorporated herein by reference in its entirety. 
     FIELD OF THE INVENTION 
     The present invention relates to nucleic acid based signaling polynucleotides (SPNs) for detecting allergen proteins. Detection agents comprising SPNs of the present invention can be used as novel biosensor platforms. 
     BACKGROUND OF THE INVENTION 
     Allergy is a serious medical condition affecting millions of people worldwide, with about 15 million people in the United States, including many children. During an allergic reaction, the immune system mistakenly targets an allergen as a threat and attacks it. The allergic reaction may affect the skin, the digestive system, the gastrointestinal tract, the respiratory system, the circulatory system and the cardiovascular system; in some allergic reactions, multiple organ systems are affected. Allergic reactions range from mild to severe or life-threatening. Severe symptoms may include difficulty in breathing, low blood pressure, chest pain, loss of consciousness, and anaphylaxis. People having allergies currently manage their allergies by avoiding any food that might contain that specific allergen. These restrictions have a major impact on the patients&#39; quality of life and there remains no method for assessing the true allergen content of food. In the United States, food allergy symptoms send someone to the emergency room every three minutes. 
     Though antibody-based immunoassays are commonly used for food allergen detection, given the fact that antibodies to allergens are not easily to be obtained, there is an unmet demand of new platform technology to improve in vitro allergen detection in both clinical and non-clinical settings. Nucleic acids, for example, nucleic acid aptamers are promising alternatives or supplements to antibodies for this purpose. These small RNA/DNA molecules can form secondary and tertiary structures capable of specifically binding proteins or other targets. Aptamers can be developed against a seemingly unlimited range of targets such as small inorganic ions, drugs, organic peptides, proteins and even complex cells. Aptamers are thermally stable, so they can be stored and transported easily. These properties make aptamers good agents for analyte detection in a sample, protein/nucleic acid purification and other aspects of biological researches. 
     Methods using aptamers specific allergen proteins have been reported for detection of several common allergens, for example, gluten (PCT Publication PCT/ES2013/000133 to Amaya-Gonzalez, et al.), and toxic ß-conglutin (Lup an 1) (Nadal, et al.,  PLoS ONE,  2012, 7(4): e35253; Nadal, et al.,  Anal. Bioanal. Chem.  2013, 405: 9343-9349; and Mairal, et al.,  Biosensors and Bioelectronics,  2014, 54: 207-210.) The present inventors have recognized that allergen detection in various matrices of food products can be conveniently performed using aptamer-based detector sequences such as signaling polynucleotides (SPNs), which are particularly well suited for use in a simple and portable sensor that can be used repetitively with high sensitivity and reproducibility at ambient temperature to ensure food safety. Recent patent applications by the inventors disclose allergen specific aptamers, SPNs and their applications in allergen detection, including PCT patent application publication NO.: WO2015/066027; PCT Patent Application Serial NO.: PCT/US 16/29356; and U.S. Patent Application Ser. No. 62/308,377; the contents of each of which are incorporated by reference in their entirety. 
     In addition to sensitive ligands (e.g., nucleic acid aptamers) that bind analytes of interest (e.g., an allergen protein), materials and surfaces that enhance the analytic performance of the ligands and biosensors are also important. Recently A wide variety of materials including magnetic particles, gold or latex particles and electrode surfaces have been used in many settings as strategies for signaling amplification. The properties of magnetic particles, both nano- and micro-size dimensions, have proved to be promising substrates to be coupled with detection ligands for the design of cost-effective biosensing platforms. 
     The present application discloses magnetic particle conjugates comprising SPNs and/or SPN complexes that are suitable for allergen detection with high sensitivity and specificity. Such SPN-magnetic particle conjugates may be used as detection agents for allergen detection in a variety of analyte detection assays, kits, devices and systems. 
     SUMMARY OF THE INVENTION 
     The present invention provides signaling polynucleotides (SPNs), SPN-complement complexes, magnetic particle conjugates, DNA printed glass slides and detection agents for allergen detection, and biosensors, assays and method for detecting allergen proteins in food samples using the compositions and agents discussed in the present disclosure. 
     In one embodiment, SPNs derived from nucleic acid aptamers having nucleic acid sequences that bind allergens with high specificity and affinity are provided. In some aspects, the signaling polynucleotides comprise nucleic acid sequences of SEQ ID NOs.: 1-696, which bind specifically to eight common food allergens. In some aspects, the SPN comprises a nucleic acid sequence that specifically binds to cashew selected from SEQ ID NOs.: 1-12. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to peanut selected from SEQ ID NOs.: 13-24, 96 and 97-496. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to tree nut selected from SEQ ID NOs.: 497-696. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to milk selected from SEQ ID NOs.: 25-34. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to fish allergen selected from SEQ ID NOs.: 35-46. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to egg selected from SEQ ID NOs.: 47-58 and 93-94. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to gluten selected from SEQ ID NOs.: 59-70 and 91-92. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to soy allergen selected from SEQ ID NOs.: 71-80. In other aspects, the SPN may comprise a nucleic acid sequence that specifically binds to crustacean selected from SEQ ID NOs.: 81-90. 
     In one embodiment, SPN-complement complexes are provided in accordance with the present invention. The SPN-complement complex comprises a SPN and a short nucleic acid sequence complementary to a portion of the sequence of the SPN, wherein the SPN and its corresponding complementary sequence are hybridized to form the complex. In one example, the complement is complementary to either the 5′ end or 3′ end sequence of the SPN. In some aspects, the complementary sequence contains about 5-20 nucleotide residues. In one preferred example, the complementary sequence contains 5-10 nucleotide residues. 
     In one embodiment, magnetic particle conjugates are provided in accordance with the present invention. The magnetic particle conjugates comprise SPN-complement complexes covalently immobilized on the surface of the particles. The magnetic particles may be micro-magnetic particles (MMPs) or nano-magnetic particles (NMPs). In some aspects, the 3′ end of the SPN is linked to magnetic particles, for example, through a 3′ amine or 3′ thiol group, or the addition of a poly-A sequence at the end, or a biotin modification. In other aspects, the 5′ end of the SPN is linked to magnetic particles, for example, through a 5′ amine or 5′ thiol group, or a biotin modification. Accordingly, the complementary sequence contains nucleotide sequences complementary to the free end of the SPN. In further other aspects, the SPN is linked to magnetic particles through its complementary sequence which is covalently immobilized on the surface of magnetic particles. In some examples, the complementary sequence may be attached to the surface of magnetic beads through amine, or thiol, or biotin-streptavidin linkage. In some aspects, magnetic particles may be further coated with PEG polymers or ethanolamine-PEG linkers. 
     In another embodiment. SPNs and SPN-complement complexes may be attached to the surface of a solid support; such solid support may be a glass slide, a silicon chip or wafer or a microwell plate. The DNA-printed surface may be used as a biosensing platform in a variety of settings for allergen detection. For example, a DNA printed glass slide may be inserted into a detection sensor for detection of a target allergen. In some aspects, one end of the SPN is attached to the two-dimensional surface of the solid support. The complement and allergen protein compete each other for binding to the attached SPN. In other aspects, the short complement is attached to the two-dimensional surface of the solid support. The SPN is hybridized with the complement to form complexes. 
     The covalent conjugation of nucleic acid molecules of the present invention (e.g., aptamer. SPN and SPN complement) to a surface, either a three-dimensional surface of magnetic particle or a two-dimensional surface of a glass slide can be through a thiol-maleimide mediated covalent chemical reaction, or an amine-mediated reaction, or a biotin-streptavidin linkage. In one example, the surface of a solid substrate (e.g. magnetic particles and glass slides) may be further coated with PEG polymers or ethanolamine-PEG linkers. 
     In some aspects, the nucleic acid sequence may be directly synthesized in situ on the solid substrate. In one embodiment, the short complementary oligonucleotide is directly synthesized on the solid substrate at a low density. A stable non-cleavable linker and optionally a spacer group may be used for in situ synthetic reaction. 
     In one embodiment, detection agents comprising SPNs, SPN-complement complexes, magnetic particle conjugates and/or DNA printed solid glasses are provided in accordance with the present invention. The detection agents may be used in any biosensor platform for capturing and detecting allergen proteins in food samples. In some aspects, the agents may be labeled with a fluorescent marker. The fluorescent marker may be conjugated to the agent in a variety of configurations. In one example, one end of the complementary sequence may be labeled with a fluorophore. In this configuration, a target allergen protein when binding to the SPN, will detach the complementary sequence hybridized with the SPN, resulting in “fluorescent signal of”. In another embodiment, one end of the complementary sequence is labeled with a quencher and the free end of the SPN is labeled with a fluorophore. Therefore, the fluorescent signal is quenched in the SPN-complement complex. The binding of a target allergen will detach the complementary sequence hybridized with the SPN, resulting in the quenched fluorescent signal back on. In further another example, one end of the complementary sequence and the free end of the SPN are labeled with different fluorophores. The binding of a target allergen will detach the complementary sequence hybridized with the SPN, resulting in a change in fluorescent signal. In each signal configuration, changes in fluorescent signals can be used to determine the presence and/or absence of the target allergen in food samples. 
     In accordance with the present invention, kits, biosensors and devices dependent on the present compositions and agents are also provided. 
     In other embodiments, the present invention provides assays and methods for detecting the presence, absence and/or quantity of an allergen of interest in a sample. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG. 1A ,  FIG. 1B  and  FIG. 1C  illustrate configurations of detection agents comprising SPN-complement complexes and magnetic particles.  FIG. 1A  displays a SPN linked to a magnetic particle through a thiol modification at the 5′end.  FIG. 1B  display a SPN linked to a magnetic particle through 3′polyA.  FIG. 1C  displays a SPN linked to a magnetic particle through an amine modification at the 5′end. 
         FIG. 2A  is a representative fluorescent signal measurement of the 5′thiol modification. C1 indicates the complementary sequence is 15 nucleotides in length, while C2 indicates that the complementary sequence is 10 nucleotides in length.  FIG. 2B  and  FIG. 2C  are representative signals of the 5′ thiol modification. C3 indicates that the complementary sequence includes 5 complementary nucleotides and 5nt polyA. 
         FIG. 3  is a diagram showing fluorescence signal off using magnetic beads conjugated with 5′thiol modified Ara H aptamer in foods spiked with different concentrations of peanut. 
         FIG. 4A ,  FIG. 4B  and  FIG. 4C  demonstrate fluorescence off; fluorescence on and dual dye configurations of SPN-complement complexes, respectively. 
         FIG. 5A  illustrates a diagram of SPN-complement complexes conjugated to the surface of a solid substrate through one end of the SPN for exposing allergen proteins;  FIG. 5B  displays a diagram of a complementary sequence conjugated to the surface of a solid substrate for exposing the SPN-allergen complexes. 
         FIG. 6A  demonstrates an attachment assay using the complementary sequence printed glass slide. The SPNs and their allergen proteins are premixed and form SPN-protein complexes. The free SPNs and SPN-protein complexes will compete with each other to attach to the complementary sequences on the surface.  FIG. 6B  demonstrates a detachment assay. The SPNs are first hybridized with the complementary sequences on the surface, forming SPN-complement complexes. Allergen proteins are added and bind to the SPNs. The SPN-protein complexes detach from the surface and will be washed off. 
         FIG. 7A  is a representative histogram of detection of peanut diluted in buffer. Peanut specific SPNs are conjugated to magnetic beads and fluorescent signals are recorded using a lab grade LED based reader. 
         FIG. 7B  is a representative histogram of detection of peanut spiked in cookie. Peanut specific SPNs are conjugated to magnetic beads and fluorescent signals are recorded using a lab grade LED based reader. 
         FIG. 8A  is a representative histogram of signal reduction when comparing food samples with and without peanut. Peanut specific SPNs are conjugated to magnetic beads and fluorescent signals are recorded using a lab grade LED based e reader. 
         FIG. 8B  is representative histogram showing signal reduction in 5 different food samples spiked with peanut at different concentrations. Peanut specific SPNs are conjugated to magnetic beads and fluorescent signals are recorded using a lab grade LED based reader. 
         FIG. 9A  shows peanut concentration curve in buffer measured on the detection assay with nucleic acid coated solid surface (chip) (N=5, LOD=1.25 ppm peanut). 
         FIG. 9B  shows peanut concentration curve in shortbread cookie measured in the detection assay with nucleic acid coated solid surface (chip) (N=3, LOD=1.25 ppm peanut). 
         FIG. 10A  demonstrates the sensitivity of detection assay with nucleic acid coated solid surface on the rig. 
         FIG. 10B  demonstrates the sensitivity of detection assay with nucleic acid solid surface on the lab-grade bench-top chip. 
         FIG. 10C  is a representative histogram showing that the same detection sensitivity is retained in diverse chocolate matrices in nucleic acid coated chip based assay on the rig. 
         FIG. 11A  demonstrates the detection signal read by the lab-grade LED reader in different food matrices in magnetic bead conjugates based assay. The food samples were prepared using GentleMACS homogenizer and processed by centrifugation. 
         FIG. 11B  demonstrates the detection signal read by the designed optic sensor in different food matrices in solid surface (chip) based assay. The food samples were prepared using GentleMACS homogenizer and processed by centrifugation. 
         FIG. 11C  demonstrates the detection signal read by the lab-grade LED based reader in 5 food matrices in magnetic bead conjugates based assay. The food samples were prepared using designed rotor homogenizer and filtered using 0.2 micron PES filter. 
         FIG. 11D  demonstrates the detection signal read by the designed optic sensor in 9 food matrices in solid surface (chip) based assay. The food samples were prepared using designed rotor homogenizer and filtered using 0.1 micron PES filter. 
         FIG. 12  demonstrates the effect of decreased reaction volume on detection sensitivity. 
         FIG. 13A  is a representative diagram of the control panels and detection area (unknown) on the chip.  FIG. 13B  is a representative signal reading of the chip.  FIG. 13C  demonstrate a representative control signal measurement. 
         FIG. 14A  depicts the effect of MgCl2 when added to the extraction buffer.  FIG. 14B  depicts the effect of MgCl2 when added after extraction and filtration.  FIG. 14C  is a representative reading of detection signals with various concentrations of MgCl2. 
         FIG. 15  shows the sensitivity in solid surface assays with optimized conditions and reduced reaction time. 
         FIGS. 16A to 16D  demonstrate the stability of reaction agents including SPNs and extraction buffer. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The details of one or more embodiments of the invention are set forth in the accompanying description below. Although any materials and methods similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred materials and methods are now described. Other features, objects and advantages of the invention will be apparent from the description. In the description, the singular forms also include the plural unless the context clearly dictates otherwise. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the case of conflict, the present description will control. 
     I. Compositions of the Present Invention 
     The present invention provides compositions, conjugates and detection agents for allergen detection. The functional/core component of a detection agent is a nucleic acid aptamer that specifically binds an allergen protein. In accordance with the present invention, an allergen specific aptamer may be modified to form a signal polynucleotide (SPN) (described in detail herein below). The SPN may be used as a free detection agent, or conjugated to a particle or a solid surface as a complex detection agent. In some aspects, the SPNs alone work as both binding ligands and signaling molecules or labels. In other aspects, complexes composed of SPNs and their complementary sequences work as both binding ligands and signaling molecules or labels. Aptamers, SPNs and/or SPN-complement complexes can be conjugated to any particles such as metal nanoparticles, redox compounds and magnetic particles, magnetic microparticles, and other solid surfaces such as glass slides and silicon chips, forming biosensor platforms. 
     Aptamers comprising nucleic acid sequences that bind common food allergens are isolated through SELEX selections. Aptamers are then modified to generate signal polynucleotides (SPNs) which work as capturing and detecting ligands for allergen proteins. In particular, the SPN may comprise a nucleic acid sequence selected from SEQ ID NOs.: 1-696. 
     In some aspects, the SPN comprises a nucleic acid sequence that specifically binds to cashew selected from SEQ ID NOs.: 1-12; the cashew specific SPN has a unique sequence of SEQ ID. NOs.: 1-6. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to peanut selected from SEQ ID NOs.: 13-24, and 96-696; the peanut specific SPN has a unique sequence of SEQ ID NOs.: 13-18 and 97-296. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to tree nut selected from SEQ ID NOs.: 497-696; the tree nut specific SPN has a unique sequence of SEQ ID NOs.: 497-596. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to milk selected from SEQ ID NOs.: 25-34; the milk specific SPN has a unique sequence of SEQ ID NOs.: 25-29. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to fish allergen selected from SEQ ID NOs.: 35-46; the fish specific SPN has a unique sequence of SEQ ID NOs.: 35-40. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to egg selected from SEQ ID NOs.: 47-58 and 93-94; the egg specific SPN has a unique sequence of SEQ ID NOs.: 47-52. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to gluten selected from SEQ ID NOs.: 59-70 and 91-92, the gluten specific SPN has a unique sequence of SEQ ID NOs.: 59-64. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to soy allergen selected from SEQ ID NOs.: 71-80; the soy specific SPN has a unique sequence of SEQ ID NOs.: 71-75. In other aspects, the SPN may comprise a nucleic acid sequence that specifically binds to crustacean selected from SEQ ID NOs.: 81-90; the crustacean specific SPN has a unique sequence of SEQ ID NOs.: 81-85. 
     In some embodiments, a SPN and a short nucleic acid sequence which is complementary to a portion of the sequence of the SPN (e.g., the sequence at either the 5′ end or 3′ end of the SPN) may be hybridized to form a complex (referred to “SPN-complement complex”). The SPN-complement complexes may be attached to the surface of magnetic particles or other solid surfaces through either the SPN or the complementary sequence. 
     In some embodiments, SPNs of the present invention are conjugated to the surface of magnetic particles or a solid substrate (e.g., a glass slide) at one end, e.g. the 5′ end or 3′end (e.g., as shown in  FIGS. 4A, 4B and 4C  and  FIG. 5A ). Nucleic acid molecules can be covalently attached to magnetic particles/beads by methods based on the formation of covalent bonds. Carboxyl and amino groups are the most common reactive groups for attaching ligands to surfaces. In some aspects, a primary amine (—NH2) modifier may be placed to the 5′end, or 3′end of a nucleic acid molecule (e.g., SPN), or internally using an amino-C or amino-T modified base. The amino-modified nucleic acid molecules may be attached to magnetic particles using an acylating reagent, for example Carbodiimide (EDC). In other aspects, a thiol group (—SH) may be attached to the 5′ or 3′ end of a nucleic acid molecule (e.g., a SPN). The thiol (—SH) modifier enables covalent attachment of a nucleic acid molecule to a variety of substrates including magnetic particles, via disulfide bond (—S—S—) or maleimide linkages. In another example, a biotinylated SPN may be conjugated to streptavidin-coated magnetic particles. In another example, PEG polymers may be introduced to the surface of a solid substrate before the conjugation. 
     In this context, the opposite end of the SPN which is free from the attached magnetic particles may be hybridized with the complementary sequence, forming a SPN-complement complex. The short complementary sequences may be labeled with a fluorescent dye, such as Alex 647, Cy5, Cy3-FITC and Texas red. The binding of an allergen to the SPN will detach the complementary sequence from the complex, resulting in a signaling change (as shown in  FIG. 5A ). 
     In some embodiments, the complementary sequences of the SPNs may be conjugated to the surface of magnetic particles or other solid substrates (e.g., printed glass surface) through covalent bindings as discussed herein. In this context, the printed glass surface having such complementary sequences may be exposed to the SPNs and allergen proteins. In this context, either an attachment assay or detachment assay may be developed for detection of allergen proteins ( FIG. 6A  and  FIG. 6B ). For example, in the attachment assay, the SPNs and allergen proteins are premixed before being exposed to the complementary sequences. Only the free SPNs will bind to the complements ( FIG. 5B  and  FIG. 6A ), generating fluorescent signals from the fluorescent dye at either the 5′end or 3′end of the SPN. As non-limiting examples, the fluorophore may be selected from Alex Fluor® fluorophores (such as Alex 514, Alex 532, Alex 546, Alex 555, Alex 568, Alex 594, Alex 610, Alex 633, Alex 635, Alex 647, Alex 660, Alex 680, Alexa 700, Alex 750, Alex 800, Alex 610-R-phycoerythrin (R-PE), Alex 647-R-phycoerythrin (R-PE), Alex 680-R-phycoerythrin (R-PE), and Alex 680-Allophycocyanin (APC)), Allophycocyanin (APC) and its derivatives, Cy fluorophores (e.g., Cy3.5, Cy3-FITC, CY5, CY 5.5, CY7, CY7-APC, CY5.5-APC), Qdots, TRITC, R-PE, Tamara, Rhodamine Red-X, Rox, TruRed, SYPRO red, BODIPY TR, Propidium iodide and Texas red. In some examples, the fluorescent dye is Alex 647, Cy5, CY3-FITC or Texas red. 
     In other embodiments, nucleic acid molecules of the present invention may be attached to solid surfaces by in situ oligonucleotide synthesis. 
     Aptamers, Signaling Polynucleotides (SPNs) and SPN-Complement Complex 
     Aptamers (sometimes also called chemical antibodies) are single-stranded oligonucleotides (RNA or single stranded DNA) that form stable but unique three-dimensional confirmations capable of binding with high affinity and specificity to a variety of molecular targets. Aptamers bind to protein targets in much the same manner as antibodies and modulate protein function. Thus, aptamers are also referred to as “chemical antibodies”. Aptamers have advantages over antibodies in that they are poorly immunogenic, stable, and often bind to a target molecule more strongly than do antibodies. Generally aptamers can be synthesized easily and in large quantities by in vitro transcription, PCR, or chemical synthesis ( Annu. Rev. Med.  2005, 56, 555-583 ; Nat. Rev. Drug Discov.  2006, 5, 123-132), and target-specific aptamers can be selected from random-sequence, single-stranded nucleic acid libraries by an in vitro selection and amplification procedure known as SELEX (systematic evolution of ligands by exponential enrichment). The selected aptamers are small single-stranded nucleic acids that fold into a well-defined three-dimensional structure. They show a high affinity and specificity for their target molecules and inhibit their biological functions. Furthermore, aptamers have important properties that simplify its industrialization. For example, aptamers are thermally stable, so they can be stored and transported easily. Aptamers can be produced or modified in large scale, with minimal batch-to-batch variation, given the well-established chemical synthesis and modification technologies. 
     Aptamers are useful and cost-effective tools for biochemical analyses. Also, they can be developed quickly against a seemingly unlimited range of targets. To date, specific aptamers against diverse targets have been successfully developed, including small inorganic irons, organic peptides, drugs, proteins, lipids and even complex cells. One of the more recent reviews of aptamer-based analysis in context of food safety control indicated that the selection of aptamers for this group of ingredients is emerging (Amaya-Gonzalez et al.,  Sensors  2013, 13: 16292-16311, the contents of which are incorporated herein by reference in its entirety). 
     1. Selection of Aptamers Specific to a Target 
     Aptamers can be artificially generated by a method called systematic evolution of ligands by exponential enrichment (SELEX) (Tuerk and Gold,  Science,  1990, 249, 505-510). More recently, a new improved separation technology for aptamer selection was introduced, capillary electrophoresis (CE)-SELEX (Mosing and Bowser,  Methods Mol Biol.,  2009, 535: 33-43). 
     Aptamers that bind to virtually any particular target can be selected by using an iterative process called SELEX™ (Systemic Evolution of Ligands by Exponential Enrichment). The process is described in, for example U.S. Pat. Nos. 5,270,163 and 5,475,096. The SELEX™ process is based on the unique insight that nucleic acids have sufficient capacity for forming a variety of two- and three-dimensional structures and sufficient chemical versatility available within their monomers to act as ligands (i.e., form specific binding pairs) with virtually any chemical compound, whether monomeric or polymeric. 
     The SELEX™ process relies, as a starting point, upon a large library or pool of single stranded oligonucleotides comprising randomized sequences. The oligonucleotides can be modified or unmodified DNA, RNA, or DNA/RNA hybrids. In some examples, the pool comprises 100% random or partially random oligonucleotides. In other examples, the pool comprises random or partially random oligonucleotides containing at least one fixed sequence and/or conserved sequence incorporated within randomized sequence. In other examples, the pool comprises random or partially random oligonucleotides containing at least one fixed sequence and/or conserved sequence at its 5′ and/or 3′ end which may comprise a sequence shared by all the molecules of the oligonucleotide pool. Fixed sequences are sequences common to oligonucleotides in the pool which are incorporated for a preselected purpose such as, CpG motifs, hybridization sites for PCR primers, promoter sequences for RNA polymerases (e.g., T3, T4, T7, and SP6), restriction sites, or homopolymeric sequences, such as poly A or poly T tracts, catalytic cores, sites for selective binding to affinity columns, and other sequences to facilitate cloning and/or sequencing of an oligonucleotide of interest. Conserved sequences are sequences, other than the previously described fixed sequences, shared by a number of aptamers that bind to the same target. 
     The oligonucleotides of the pool preferably include a randomized sequence portion as well as fixed sequences necessary for efficient amplification. Typically, the oligonucleotides of the starting pool contain fixed 5′ and 3′ terminal sequences which flank an internal region of 30-50 random nucleotides. The randomized nucleotides can be produced in a number of ways including chemical synthesis and size selection from randomly cleaved cellular nucleic acids. Sequence variation in the test nucleic acids can also be introduced or increased by mutagenesis before or during the selection/amplification iterations. 
     The random sequence portion of the oligonucleotide can be of any length and can comprise ribonucleotides and/or deoxyribonucleotides and can include modified or non-natural nucleotides or nucleotide analogs (see for example U.S. Pat. Nos. 5,958,691 and 5,660,985). Random oligonucleotides can be synthesized from phosphodiester-linked nucleotides using solid phase oligonucleotide synthesis techniques well known in the art. Random oligonucleotides can also be synthesized using solution phase methods such as triester synthesis methods. Typical syntheses carried out on automated DNA synthesis equipment yield 10 14 -10 16  individual molecules, a number sufficient for most SELEX™ experiments. 
     The starting library of oligonucleotides may be generated by automated chemical synthesis on a DNA synthesizer. Partially random sequences can be created by adding the four nucleotides in different molar ratios at each addition step. 
     The library of oligonucleotides for aptamer selection may be either RNA or DNA. A RNA library of oligonucleotides is typically generated by transcribing a DNA library of oligonucleotides in vitro using T7 RNA polymerase or modified T7 RNA polymerases and purified. The RNA or DNA library is then mixed with the target under conditions favorable for binding and subjected to step-wise iterations of binding, partitioning and amplification, using the same general selection scheme, to achieve the desired criterion of binding affinity and selectivity as defined in the present application. 
     More specifically, starting with a mixture containing the starting pool of nucleic acids, the SELEX™ method includes steps of: (a) contacting the mixture with the target under conditions favorable for binding; (b) partitioning unbound nucleic acids from those nucleic acids which have bound specifically to target molecules; (c) dissociating the nucleic acid-target complexes; (d) amplifying the nucleic acids dissociated from the nucleic acid-target complexes to yield a ligand-enriched mixture of nucleic acids; and (e) reiterating the steps of binding, partitioning, dissociating and amplifying through as many cycles as desired to yield highly specific, high affinity nucleic acid ligands to the target molecule. Cycles of selection and amplification are repeated until a desired goal is achieved. Generally this is until no significant improvement in binding strength is achieved on repetition of the cycle. Typically, nucleic acid aptamer molecules are selected in a 5 to 20 cycle procedure. 
     A variety of nucleic acid primary, secondary and tertiary structures are known to exist. The structures or motifs that have been shown most commonly to be involved in non-Watson-Crick type interactions are referred to as hairpin loops, symmetric and asymmetric bulges, pseudoknots and myriad combinations of the same. The core SELEX™ method has been modified to achieve a number of specific objectives, such as selection of aptamers with particular secondary structures. Examples of SELEX processes can be found in U.S. Pat. Nos. 5,270,163 and 5,475,096. For example, U.S. Pat. No. 5,707,796 describes the use of SELEX™ in conjunction with gel electrophoresis to select nucleic acid molecules with specific structural characteristics, such as bent DNA. U.S. Pat. No. 5,763,177 describes SELEX™ based methods for selecting nucleic acid ligands containing photo reactive groups capable of binding and/or photo-crosslinking to and/or photo-inactivating a target molecule U.S. Pat. Nos. 5,567,588 and 5,861,254 describe SELEX™ based methods which achieve highly efficient partitioning between oligonucleotides having high and low affinity for a target molecule. U.S. Pat. No. 5,496,938 describes methods for obtaining improved nucleic acid ligands after the SELEX™ process has been performed. U.S. Pat. No. 5,705,337 describes methods for covalently linking a ligand to its target, the contents of each of which are incorporated herein by reference in their entirety. 
     Counter-SELEX™ is a method for improving the specificity of nucleic acid ligands to a target molecule by eliminating nucleic acid ligand sequences with cross-reactivity to one or more non-target molecules. Counter-SELEX™ is comprised of the steps of: (a) preparing a candidate mixture of nucleic acids; (b) contacting the candidate mixture with the target, wherein nucleic acids having an increased affinity to the target relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; (c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; (d) dissociating the increased affinity nucleic acids from the target; (e) contacting the increased affinity nucleic acids with one or more non-target molecules such that nucleic acid ligands with specific affinity for the non-target molecule(s) are removed; and (f) amplifying the nucleic acids with specific affinity only to the target molecule to yield a mixture of nucleic acids enriched for nucleic acid sequences with a relatively higher affinity and specificity for binding to the target molecule. As described above for SELEX™, cycles of selection and amplification are repeated as necessary until a desired goal is achieved. 
     The binding affinity describes the measure of the strength of the binding or affinity of molecules to each other. Binding affinity of the aptamer herein with respect to targets and other molecules is defined in terms of K d . The dissociation constant can be determined by methods known in the art. It has been observed, however, that for some small oligonucleotides, direct determination of K d  is difficult, and can lead to misleadingly high results. Under these circumstances, a competitive binding assay for the target molecule or other candidate substance can be conducted with respect to substances known to bind the target or candidate. The value of the concentration at which 50% inhibition occurs (K,) is, under ideal conditions, equivalent to K d . 
     In accordance with the present invention, a SELEX approach was used to select core binding aptamers that bind 8 major food allergens (i.e. cashew, egg, milk, peanuts, gluten, fish, crustacean and soy). Several aptamers with sequences that can specifically recognize a target allergen were selected and the nucleic acid sequences of selected aptamers were further modified to generate signaling polynucleotides. The aptamers with high selectivity, specificity and stability are selected and further labeled as detection agents. The sequences of the selected aptamers for the 8 major allergens are listed in Table 1. For example, an aptamer having a full sequence (SEQ ID NO.: 7) is one of the SPNs that bind cashew. The full sequence includes the primers used for the screen and the core binding sequence of the aptamer (SEQ ID NO.: 1), the full sequence will be further modified to generate signaling polynucleotides (SPNs) specific to cashew, as discussed herein below. 
     2. Aptamer Modifications 
     In accordance with the present invention, oligonucleotides and aptamers may be further modified to improve their stability. The present invention also includes analogs as described herein and/or additional modifications designed to improve one or more characteristics of aptamers such as protection from nuclease digestion. Oligonucleotide modifications contemplated in the present invention include, but are not limited to, those which provide other chemical groups that incorporate additional charge, polarizability, hydrophobicity, hydrogen bonding, electrostatic interaction, and fluxionality to the nucleic acid ligand bases or to the nucleic acid ligand as a whole. 
     Modifications to generate oligonucleotides which are resistant to nucleases can also include one or more substitute internucleotide linkages, altered sugars, altered bases, or combinations thereof. Such modifications include 2′-position sugar modifications, 5-position pyrimidine modifications, 8-position purine modifications, modifications at exocyclic amines, substitution of 4-thiouridine, substitution of 5-bromo or 5-iodo-uracil, backbone modifications, phosphorothioate or alkyl phosphate modifications, methylations, and unusual base-pairing combinations such as the isobases isocytidine and isoguanosine, 3′ and 5′ modifications such as capping; conjugation to a high molecular weight, non-immunogenic compound; conjugation to a lipophilic compound; and phosphate backbone modification. 
     Nucleic acid aptamers may be ribonucleic acid, deoxyribonucleic acid, or mixed ribonucleic acid and deoxyribonucleic acid. Aptamers may be single stranded ribonucleic acid, deoxyribonucleic acid or mixed ribonucleic acid and deoxyribonucleic acid. 
     Nucleic acid aptamers comprise a series of linked nucleosides or nucleotides. The term “nucleic acid,” in its broadest sense, includes any compound and/or substance that comprise a polymer of nucleotides. These polymers are often referred to as polynucleotides. Exemplary nucleic acid molecules or polynucleotides of the invention include, but are not limited to, either D- or L-nucleic acids, ribonucleic acids (RNAs), deoxyribonucleic acids (DNAs), threose nucleic acids (TNAs), glycol nucleic acids (GNAs), peptide nucleic acids (PNAs), locked nucleic acids (LNAs, including LNA having a β-D-ribo configuration, α-LNA having an α-L-ribo configuration (a diastereomer of LNA), 2′-amino-LNA having a 2′-amino functionalization, and 2′-amino-α-LNA having a 2′-amino functionalization) or hybrids thereof. 
     The skilled artisan will recognize that the term “RNA molecule” or “ribonucleic acid molecule” encompasses not only RNA molecules as expressed or found in nature, but also analogs and derivatives of RNA comprising one or more ribonucleotide/ribonucleoside analogs or derivatives as described herein or as known in the art. Strictly speaking, a “ribonucleoside” includes a nucleoside base and a ribose sugar, and a “ribonucleotide” is a ribonucleoside with one, two or three phosphate moieties. However, the terms “ribonucleoside” and “ribonucleotide” can be considered to be equivalent as used herein. The RNA can be modified in the nucleobase structure, the ribofuranosyl ring or in the ribose-phosphate backbone. 
     In some embodiments, the aptamer comprises at least one chemical modification. In some embodiments, the chemical modification is selected from a chemical substitution of the nucleic acid at a sugar position, a chemical substitution at a phosphate position and a chemical substitution at a base position. In other embodiments, the chemical modification is selected from incorporation of a modified nucleotide; 3′ capping; conjugation to a high molecular weight, non-immunogenic compound; conjugation to a lipophilic compound; and incorporation of phosphorothioate into the phosphate backbone. In a preferred embodiment, the high molecular weight, non-immunogenic compound is polyalkylene glycol, and more preferably is polyethylene glycol (PEG). The process of covalent conjugation of PEG to another molecule, normally a drug or therapeutic protein is known as PEGylation. PEGylation is routinely achieved by incubation of a reactive derivative of PEG with the target molecule. The covalent attachment of PEG to a drug or therapeutic protein can mask the agent from the host&#39;s immune system, thereby providing reduced immunogenicity and antigenicity, and increase the hydrodynamic size (size in solution) of the agent which prolongs its circulatory time by reducing renal clearance. PEGylation can also provide water solubility to hydrophobic drugs and proteins. 
     In another preferred embodiment, the 3′ cap is an inverted deoxythymidine cap. 
     In some embodiments, nucleic acid aptamers are provided in which the P(O)O group is replaced by P(O)S (“thioate”), P(S)S (“dithioate”). P(O)NR2 (“amidate”), P(O)R, P(O)OR′, CO or CH2 (“formacetal”) or 3′-amine (—NH—CH2-CH2-), wherein each R or R′ is independently H or substituted or unsubstituted alkyl. Linkage groups can be attached to adjacent nucleotide through a —O—, —N—, or —S— linkage. Not all linkages in the nucleic acid aptamers are required to be identical. 
     As non-limiting examples, a nucleic acid aptamer can include D-ribose or L-ribose nucleic acid residues and can also include at least one modified ribonucleoside including but not limited to a 2′-O-methyl modified nucleoside, a nucleoside comprising a 5′ phosphorothioate group, a terminal nucleoside linked to a cholesteryl derivative or dodecanoic acid bisdecylamide group, a locked nucleoside, an abasic nucleoside, an inverted deoxynucleoside or inverted ribonucleoside, a 2′-deoxy-2′-fluoro-modified nucleoside, a 2′-amino-modified nucleoside, a 2′-alkyl-modified nucleoside, a morpholino nucleoside, a phosphoramidate or a non-natural base comprising nucleoside, or any combination thereof. Alternatively, a nucleic acid aptamer can comprise at least two modified ribonucleosides, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 15, at least 20 or more modified ribonucleosides, up to the entire length of the molecule. The modifications need not be the same for each of such a plurality of modified deoxy- or ribonucleosides in a nucleic acid molecule. 
     Aptamer may comprise modified nucleobase (often referred to in the art simply as “base”) for increasing the affinity and specificity for their target protein. As used herein, “unmodified” or “natural” nucleobases include the purine bases adenine (A) and guanine (G), and the pyrimidine bases thymine (T), cytosine (C) and uracil (U). For example, the modified base may be a pyrimidine modified by a hydrophobic group, such as benzyl group, a naphthyl group, or a pyrrolebenzyl group, at its 5-position. Modified nucleoside may be exemplified as 5-(N-benzylcarboxyamide)-2′-deoxyuridine (called BzdU), 5-(N-naphthylcarboxyamide)-2′-deoxyuridine (called NapdU), 5-(N-4-pyrrolebenzyl carboxyamide)-2′-deoxyuridine (called 4-PBdU), 5-(N-benzylcarboxyamide)-2′-deoxycytidine (called BzdC), 5-(N-naphthylcarboxyamide)-2′-deoxycytidine (called NapdC), 5-(N-4-pyrrolebenzylcarboxyamide)-2′-deoxycytidine (called 4-PBdC), 5-(N-benzylcarboxyamide)-2′-uridine (called BzU), 5-(N-naphthylcarboxyamide)-2′-uridine (called NapU), 5-(N-4-pyrrolebenzylcarboxyamide)-2′-uridine (called 4-PBU), 5-(N-benzylcarboxyamide)-2′-cytidine (called BzC), 5-(N-naphthylcarboxyamide)-2′-cytidine (called NapC), 5-(N-4-pyrrolebenzyl carboxyamide)-2′-cytidine (called 4-PBC), and the like, but not be limited thereto. Further nucleobases include those disclosed in U.S. Pat. No. 3,687,808, those disclosed in Modified Nucleosides in Biochemistry, Biotechnology and Medicine, Herdewijn, P. ed. Wiley-VCH, 2008; those disclosed in The Concise Encyclopedia Of Polymer Science And Engineering, pages 858-859, Kroschwitz, J. L, ed. John Wiley &amp; Sons, 1990, those disclosed by Englisch et al.,  Angewandte Chemie , International Edition, 1991, 30, 613, and those disclosed by Sanghvi, Y S., Chapter 15 , dsRNA Research and Applications , pages 289-302, Crooke, S. T. and Lebleu, B., Ed., CRC Press, 1993. 
     In accordance with the present invention, a suitable nucleotide length for an aptamer ranges from about 15 to about 100 nucleotides (nt), and in various other preferred embodiments, 15-30 nt, 20-25 nt, 30-100 nt, 30-60 nt, 25-70 nt, 25-60 nt, 40-60 nt, 25-40 nt, 30-40 nt, any of 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 or 40 nt or 40-70 nt in length. In some embodiments, an aptamer may be 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, or 70 nt in length. In other embodiments, an aptamer may 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100 nt in length. However, the sequence can be designed with sufficient flexibility such that it can accommodate interactions of aptamers with two targets at the distances described herein. 
     In some embodiments, the nucleic acid aptamer comprises one or more regions of double-stranded character. Such double stranded regions may arise from internal self-complementarity or complementarity with a second or further aptamers or oligonucleotide molecule. In some embodiments the double stranded region may be from 4-12, 4-10, 4-8 base pairs in length. In some embodiments the double stranded region may be 5, 6, 7, 8, 9, 10, 11 or 12 base pairs. In some embodiments the double stranded region may form a stem region. Such extended stem regions having double stranded character can serve to stabilize the nucleic acid aptamer. As used herein, the term “double stranded character” means that over any length of two nucleic acid molecules, their sequences form base pairings (standard or nonstandard) of more than 50 percent of the length. 
     Aptamers may be further modified to provide protection from nuclease and other enzymatic activities. The aptamer sequence can be modified by any suitable methods known in the art. For example, phosphorothioate can be incorporated into the backbone, and 5′-modified pyrimidine can be included in 5′ end of ssDNA for DNA aptamers. For RNA aptamers, modified nucleotides such as substitutions of the 2′-OH groups of the ribose backbone, e.g., with 2′-deoxy-NTP or 2′-fluoro-NTP, can be incorporated into the RNA molecule using T7 RNA polymerase mutants. The resistance of these modified aptamers to nuclease can be tested by incubating them with either purified nucleases or nuclease from mouse serum, and the integrity of aptamers can be analyzed by gel electrophoresis. 
     In some embodiments, such modified nucleic acid aptamers may be synthesized entirely of modified nucleotides, or with a subset of modified nucleotides. The modifications can be the same or different. All nucleotides may be modified, and all may contain the same modification. All nucleotides may be modified, but contain different modifications, e.g., all nucleotides containing the same base may have one type of modification, while nucleotides containing other bases may have different types of modifications. For example, all purine nucleotides may have one type of modification (or are unmodified), while all pyrimidine nucleotides have another, different type of modification (or are unmodified). In this way, oligonucleotides, or libraries of oligonucleotides are generated using any combination of modifications as disclosed herein. 
     Aptamers may be either monovalent or multivalent. Aptamers may be monomeric, dimeric, trimeric, tetrameric or higher multimeric. Individual aptamer monomers may be linked to form multimeric aptamer fusion molecules. As a non-limiting example, a linking oligonucleotide (i.e., linker) may be designed to contain sequences complementary to both 5′-arm and 3′-arm regions of random aptamers to form dimeric aptamers. For trimeric or tetrameric aptamers, a small trimeric or tetrameric (i.e., a Holiday junction-like) DNA nanostructure will be engineered to include sequences complementary to the 3′-arm region of the random aptamers, therefore creating multimeric aptamer fusion through hybridization. In addition, 3 to 5 or 5 to 10 dT rich nucleotides can be engineered into the linker polynucleotides as a single stranded region between the aptamer-binding motifs, which offers flexibility and freedom of multiple aptamers to coordinate and synergize multivalent interactions with cellular ligands or receptors. Alternatively, multimeric aptamers can also be formed by mixing biotinylated aptamers with streptavidin. 
     As used herein, the term “multimeric aptamer” or “multivalent aptamer” refers to an aptamer that comprises multiple monomeric units, wherein each of the monomeric units can be an aptamer on its own. Multivalent aptamers have multivalent binding characteristics. A multimeric aptamer can be a homomultimer or a heteromultimer. The term “homomultimer” refers to a multimeric aptamer that comprises multiple binding units of the same kind, i.e., each unit binds to the same binding site of the same target molecule. The term “heteromultimer” refers to a multimeric aptamer that comprises multiple binding units of different kinds, i.e., each binding unit binds to a different binding site of the same target molecule, or each binding unit binds to a binding site on different target molecule. Thus, a heteromultimer can refer to a multimeric aptamer that binds to one target molecule at different binding sties or a multimeric aptamer that binds to different target molecules. A heteromultimer that binds to different target molecules can also be referred to as a multi-specific multimer. 
     According to certain embodiments of the present invention, variants and derivatives of aptamers are provided. The term “derivative” is used synonymously with the term “variant” and refers to a molecule that has been modified or changed in any way relative to a reference or starting aptamer. The nucleic acid sequence of aptamer variants may possess substitutions, deletions, and/or insertions at certain positions within the nucleotide sequence, as compared to a reference or starting sequence. Ordinarily, variants will possess at least about 50% identity (homology) to a reference sequence, and preferably, they will be at least about 80%, more preferably at least about 90% identical (homologous) to a reference sequence. 
     In some embodiments, variant mimics of aptamers of the present invention are provided. As used herein, the term “variant mimic” is one which contains one or more nucleic acids which would mimic an activated sequence. The nucleic acid sequences of variant mimics may comprise naturally occurring nucleic acids, or alternatively, non-naturally occurring nucleic acids. 
     3. Signaling Polynucleotides (SPNs) 
     Aptamers selected through the process mentioned above herein may be used as signaling polynucleotides (SPNs) for detection of target allergens. Signaling polynucleotides based on aptamer core/binding sequences are advantageous with respect to the objective of development of simple, yet effective detection assays for biomolecule sensors. In accordance with the present invention, a signaling polynucleotide may be developed from the selected aptamers which specifically bind a target allergen molecule. The polynucleotide sequences are detectable when bound at high affinity and specificity to molecular targets. 
     In some embodiments, signaling polynucleotides (SPNs) of the present invention comprise the core binding sequences which determine the specificity and affinity of SPNs to a target allergen molecule. The full sequence of a selected aptamer can be shortened by deleting the primers used for aptamer selection without impacting the binding sequence to a target allergen. Additional nucleotides may also be added at the 5′terminus and/or the 3′ terminus, without impacting the binding (core) sequence of each aptamer. 3D structures of such SPNs are predicted using standard structure prediction software. The resulting polynucleotide may form a stable 3D structure. In other aspects, nucleotides added at the termini may increase the stability of the polynucleotide and facilitate magnetic particle conjugation, and/or other modifications. For example, a short polyA sequence may be added to the terminus of a SPN to increase the distance between the SPN and magnetic particles (or other solid surface). The length and sequence of additional nucleotides may vary in the context of the core binding sequence of a signaling polynucleotide. SPNs generated from aptamers against common allergens are listed in Tables 1-4. 
     In some aspects, the SPN comprises a nucleic acid sequence that specifically binds to cashew selected from SEQ ID NOs.: 1-12; the cashew specific SPN has a unique sequence of SEQ ID. NOs.: 1-6. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to peanut selected from SEQ ID NOs.: 13-24, and 97-496; the peanut specific SPN has a unique sequence of SEQ ID NOs.: 13-18 and 97-296. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to tree nut selected from SEQ ID NOs.: 497-696; the tree nut specific SPN has a unique sequence of SEQ ID NOs.: 497-596. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to milk selected from SEQ ID NOs.: 25-34; the milk specific SPN has a unique sequence of SEQ ID NOs.: 25-29. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to fish allergen selected from SEQ ID NOs.: 35-46; the fish specific SPN has a unique sequence of SEQ ID NOs.: 35-40. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to egg selected from SEQ ID NOs.: 47-58 and 93-94; the egg specific SPN has a unique sequence of SEQ ID NOs.: 47-52. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to gluten selected from SEQ ID NOs.: 59-70 and 91-92; the gluten specific SPN has a unique sequence of SEQ ID NOs.: 59-64. In some aspects, the SPN may comprise a nucleic acid sequence that specifically binds to soy allergen selected from SEQ ID NOs.: 71-80; the soy specific SPN has a unique sequence of SEQ ID NOs.: 71-75. In other aspects, the SPN may comprise a nucleic acid sequence that specifically binds to crustacean selected from SEQ ID NOs.: 81-90; the crustacean specific SPN has a unique sequence of SEQ ID NOs.: 81-85. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Aptamers and SPNs that bind common allergens 
               
            
           
           
               
               
               
               
            
               
                 SEQ ID NO. 
                 Core sequence (5′-3′) 
                 SEQ ID NO. 
                 Full Sequence (5′-3′) 
               
               
                   
               
            
           
           
               
            
               
                 Cashew 
               
            
           
           
               
               
               
               
            
               
                  1 
                 GCACACCACGTCAAAAATCATTGTCACC 
                  7 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGGC 
               
               
                   
                 ACGAAGC 
                   
                 ACACCACGTCAAAAATCATTGTCACCACGAAGCCG 
               
               
                   
                   
                   
                 AAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                  2 
                 TGCGCAACATAAGTCTCTTGAAAGACCA 
                  8 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTG 
               
               
                   
                 CGTTCAA 
                   
                 CGCAACATAAGTCTCTTGAAAGACCACGTTCAACG 
               
               
                   
                   
                   
                 AAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                  3 
                 CACCCACCATACCAGAAATGTTGACACC 
                  9 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCA 
               
               
                   
                 ACGTGGA 
                   
                 CCCACCATACCAGAAATGTTGACACCACGTGGACG 
               
               
                   
                   
                   
                 AAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                  4 
                 TGCACAATGTAATTATCAAAATACACCA 
                 10 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTG 
               
               
                   
                 CGGTTGC 
                   
                 CACAATGTAATTATCAAAATACACCACGTTGGCCG 
               
               
                   
                   
                   
                 AAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                  5 
                 CCACATCGTGCAATGCCCGAAACATACC 
                 11 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                 ACGTAGA 
                   
                 ACATCGTGCAATGCCCGAAACATACCACGTAGACG 
               
               
                   
                   
                   
                 AAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                  6 
                 CTATGCAGTGATGATTAAAGATACCACC 
                 12 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCT 
               
               
                   
                 ACGTGAG 
                   
                 ATGCAGTGATGATTAAAGATACCACCACGTGAGCG 
               
               
                   
                   
                   
                 AAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                   
               
            
           
           
               
            
               
                 Peanut 
               
            
           
           
               
               
               
               
            
               
                 13 
                 CAAATAGTTACAAACACCACGTAG 
                 19 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCA 
               
               
                   
                   
                   
                 AATAGTTACAAACACCACGTAGCGAAACGTGGTGA 
               
               
                   
                   
                   
                 AAGCCACGTAGCTGCGCC 
               
               
                 14 
                 CCCAACTGTACAGTACACCACGTAG 
                 20 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                   
                   
                 CAACTGTACAGTACACCACGTAGCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 15 
                 CACACACACATTCCACCACGTCACG 
                 21 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCA 
               
               
                   
                   
                   
                 CACACACATTCCACCACGTCACGCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 16 
                 CACACGTTACCACACCACGTTGACG 
                 22 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCA 
               
               
                   
                   
                   
                 CACGTTACCACACCACGTTGACGCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 17 
                 CGTGCCCGAAACACACACCACGATG 
                 23 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCG 
               
               
                   
                   
                   
                 TGCCCGAAACACACACCACGATGCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 18 
                 CTCACCACATACCATGTACCACGTG 
                 24 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCT 
               
               
                   
                   
                   
                 CACCACATACCATGTACCACGTGCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                   
               
            
           
           
               
            
               
                 Milk 
               
            
           
           
               
               
               
               
            
               
                 25 
                 TTCACTGGCTGCACCCACCACCGCGTTC 
                 30 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTT 
               
               
                   
                 CA 
                   
                 CACTGGCTGCACCCACCACCGCGTTCCACGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 26 
                 CATCCACGGTGACGCTAATCCCACGTTC 
                 31 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCA 
               
               
                   
                 GA 
                   
                 TCCACGGTGACGCTAATCCCACGTTCGACGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 27 
                 ACAATGCAGATGCGCCCACCACGGATCA 
                 32 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGAC 
               
               
                   
                 CT 
                   
                 AATGCAGATGCGCCCACCACGGATCACTCGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 28 
                 CAACCAAGCACGCTGCATCACGTTTCAT 
                 33 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCA 
               
               
                   
                 CG 
                   
                 ACCAAGCACGCTGCATCACGTTTCATCGCGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 29 
                 CTCACAGCCCGAAACACATCGCCACGTT 
                 34 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCT 
               
               
                   
                 CA 
                   
                 CACAGCCCGAAACACATCGCCACGTTCACGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                   
               
            
           
           
               
            
               
                 Fish 
               
            
           
           
               
               
               
               
            
               
                 35 
                 CTCAATACTACGTCAATTCACAGATGAT 
                 41 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCT 
               
               
                   
                 AGACACCACGGA 
                   
                 CAATACTACGTCAATTCACAGATGATAGACACCAC 
               
               
                   
                   
                   
                 GGACGAAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 36 
                 TCCAACACCACGTAACGTACACTGCATG 
                 42 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTC 
               
               
                   
                 TGATTGGTGCAA 
                   
                 CAACACCACGTAACGTACACTGCATGTGATTGGTG 
               
               
                   
                   
                   
                 CAACGAAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 37 
                 TGGCGCCGACTGATCAACTAGACATCAC 
                 43 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTG 
               
               
                   
                 GTTAGCATTCCG 
                   
                 GCGCCGACTGATCAACTAGACATCACGTTAGCATT 
               
               
                   
                   
                   
                 CCGCGAAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 38 
                 CCAGCAACCAGGTTACCTCCCATCACGC 
                 44 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                 TTCGTCTCAGGA 
                   
                 AGCAACCAGGTTACCTCCCATCACGCTTCGTCTCA 
               
               
                   
                   
                   
                 GGACGAAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 39 
                 CTGACACCACAAACGATTATGACCACGT 
                 45 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCT 
               
               
                   
                 TATCGTACATAG 
                   
                 GACACCACAAACGATTATGACCACGTTATCGTACA 
               
               
                   
                   
                   
                 TAGCGAAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 40 
                 TAGGTCAAGTGCGCTAAAACACACCGCG 
                 46 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTA 
               
               
                   
                 TTAGTTCACCAA 
                   
                 GGTCAAGTGCGCTAAAACACACCGCGTTAGTTCAC 
               
               
                   
                   
                   
                 CAACGAAACGTGGTGAAAGCCACGTAGCTGCGCC 
               
               
                   
               
            
           
           
               
            
               
                 Egg 
               
            
           
           
               
               
               
               
            
               
                 47 
                 GGCCACCTCACTGTGTTTTGTTGCACAA 
                 53 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGGC 
               
               
                   
                 CATAATATGATGACGTGC 
                   
                 CACCTCACTGTGTTTTGTTGCACAACATAATATGA 
               
               
                   
                   
                   
                 TGACGTGCCGAAACGTGGTGAAAGCCACGTAGCTG 
               
               
                   
                   
                   
                 CGCC 
               
               
                 48 
                 GCGTTCCCCACCGTTGCCCACGCTTAAC 
                 54 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGGC 
               
               
                   
                 TGGACAAAGATGGGCCC 
                   
                 GTTCCCCACCGTTGCCCACGCTTAACTGGACAAAG 
               
               
                   
                   
                   
                 ATGGGCCCCGAAACGTGGTGAAAGCCACGTAGCTG 
               
               
                   
                   
                   
                 CGCC 
               
               
                 49 
                 TCTGTGCACATCACTCGACCTCTACGGC 
                 55 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTC 
               
               
                   
                 TGTATTGATCCTGCATA 
                   
                 TGTGCACATCACTCGACCTCTACGGCTGTATTGAT 
               
               
                   
                   
                   
                 CCTGCATACGAAACGTGGTGAAAGCCACGTAGCTG 
               
               
                   
                   
                   
                 CGCC 
               
               
                 50 
                 CGTCCAACGTTCGATCAGAACCGCGTTC 
                 56 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCG 
               
               
                   
                 AGGCTGATGATTGTACG 
                   
                 TCCAACGTTCGATCAGAACCGCGTTCAGGCTGATG 
               
               
                   
                   
                   
                 ATTGTACGCGAAACGTGGTGAAAGCCACGTAGCTG 
               
               
                   
                   
                   
                 CGCC 
               
               
                 51 
                 CATCAGTGCGTTCTGCCTTTGCAACCAC 
                 57 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCA 
               
               
                   
                 ACAACACACCGTATGAG 
                   
                 TCAGTGCGTTCTGCCTTTGCAACCACACAACACAC 
               
               
                   
                   
                   
                 CGTATGAGCGAAACGTGGTGAAAGCCACGTAGCTG 
               
               
                   
                   
                   
                 CGCC 
               
               
                 52 
                 CCAACTGTGCACACTGTTCGCTTATCGA 
                 58 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                 GCTGTGTACCTCCATAG 
                   
                 AACTGTGCACACTGTTCGCTTATCGAGCTGTGTAC 
               
               
                   
                   
                   
                 CTCCATAGCGAAACGTGGTGAAAGCCACGTAGCTG 
               
               
                   
                   
                   
                 CGCC 
               
               
                   
               
            
           
           
               
            
               
                 Gluten 
               
            
           
           
               
               
               
               
            
               
                 59 
                 CTTGGTCACCTTTCCTGACATTAACACA 
                 65 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCT 
               
               
                   
                 GG 
                   
                 TGGTCACCTTTCCTGACATTAACACAGGCGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 60 
                 TTTTCCCGATACGGCTACGAATTGCGAC 
                 66 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTT 
               
               
                   
                 AA 
                   
                 TTCCCGATACGGCTACGAATTGCGACAACGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 61 
                 GCACCAATTTTACCGATTTTGGTGGACA 
                 67 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGGC 
               
               
                   
                 GC 
                   
                 ACCAATTTTACCGATTTTGGTGGACAGCCGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 62 
                 CGTACAACCCACCACCGTTGTCCACAAA 
                 68 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCG 
               
               
                   
                 TG 
                   
                 TACAACCCACCACCGTTGTCCACAAATGCGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 63 
                 TGCGTCAACGGCCGTCCCGAAACGTGAA 
                 69 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGTG 
               
               
                   
                 TA 
                   
                 CGTCAACGGCCGTCCCGAAACGTGAATACGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                 64 
                 GTTACCCCGAAACGGCCCTAACTGCATC 
                 70 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGGT 
               
               
                   
                 AG 
                   
                 TACCCCGAAACGGCCCTAACTGCATCAGCGAAACG 
               
               
                   
                   
                   
                 TGGTGAAAGCCACGTAGCTGCGCC 
               
               
                   
               
            
           
           
               
            
               
                 Soy 
               
            
           
           
               
               
               
               
            
               
                 71 
                 CCGCATCACCACCCAAACCACCGTT 
                 76 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                   
                   
                 GCATCACCACCCAAACCACCGTTCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 72 
                 CCTGCTCCATCCGCGCCAGCCTCAC 
                 77 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                   
                   
                 TGCTCCATCCGCGCCAGCCTCACCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 73 
                 CCAATCTCCTGCCCACGCCGTTCCA 
                 78 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                   
                   
                 AATCTCCTGCCCACGCCGTTCCACGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 74 
                 CCAATCAAGGACCGCCTTCACCGCT 
                 79 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                   
                   
                 AATCAAGGACCGCCTTCACCGCTCGAAACGTGGTG 
               
               
                   
                   
                   
                 GAAAGCCACGTAGCTGCGCC 
               
               
                 75 
                 ACTCTCGCATCACCAGCCAACTCAC 
                 80 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGAC 
               
               
                   
                   
                   
                 TCTCGCATCACCAGCCAACTCACCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                   
               
            
           
           
               
            
               
                 Crustacean 
               
            
           
           
               
               
               
               
            
               
                 81 
                 CGGTACTCAGATTACAGAGTGACAT 
                 86 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCG 
               
               
                   
                   
                   
                 GTACTCAGATTACAGAGTGACATCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 82 
                 AGACACCACGGATCCGAACTGGAG 
                 87 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGAG 
               
               
                   
                   
                   
                 ACACCACGGATCCGAACTGGAGCGAAACGTGGTGA 
               
               
                   
                   
                   
                 AAGCCACGTAGCTGCGCC 
               
               
                 83 
                 CCTCGCAAGATTGCATACGTTAGAA 
                 88 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                   
                   
                 TCGCAAGATTGCATACGTTAGAACGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 84 
                 CACGTAGGAAACGACCTCTACGGAG 
                 89 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCA 
               
               
                   
                   
                   
                 CGTAGGAAACGACCTCTACGGAGCGAAACGTGGTG 
               
               
                   
                   
                   
                 AAAGCCACGTAGCTGCGCC 
               
               
                 85 
                 CCCGAAACCACCACCGTTGTCCAATA 
                 90 
                 TAATACGACTCACTATAGGCGTAGCCTGATGAGCC 
               
               
                   
                   
                   
                 CGAAACCACCACCGTTGTCCAATACGAAACGTGGT 
               
               
                   
                   
                   
                 GAAAGCCACGTAGCTGCGCC 
               
               
                   
               
            
           
         
       
     
     In some embodiments, signaling polynucleotides of the present invention may be generated by modifying the original allergen binding aptamers disclosed in the literature. The parent sequence of each aptamer against a specific allergen is modified to comprise the shortest sequence without changing the binding specificity and affinity of the aptamer. Some exemplary signaling polynucleotides modified from known parent sequences are listed in Table 2. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 SPNs originated from literature sequences 
               
            
           
           
               
               
               
               
            
               
                 Allergen 
                 Description 
                 SEQ ID NO. 
                 Sequence (5′-3′) 
               
               
                   
               
               
                 Gluten 
                 GLI4- 
                 91 
                 CCAGTCTCCCGTTTACCGCGCCTACACATGT 
               
               
                   
                 aptamersequence 
                   
                 CTGAATGCC 
               
               
                   
                 GLI4- 
                 92 
                 CTAGGCGAAATATAGCTACAACTGTCTGAAG 
               
               
                   
                 aptamersequence 
                   
                 GCACCCAAT 
               
               
                   
               
               
                 Egg 
                 Aptamersequence 
                 93 
                 ATCTACGAATTCATCAGGGCTAAAGAGTGCA 
               
               
                   
                   
                   
                 GAGTTACTTAG 
               
               
                   
                 Aptamer 
                 94 
                 GCAGCTAAGCAGGCGGCTCACAAAACCATTC 
               
               
                   
                 sequence 
                   
                 GCATGCGGC 
               
               
                   
               
               
                 Ellington 
                 apatmer 
                 95 
                 GGUUGUGAAGAUUGGGAGCGUCGUGGCUAC 
               
               
                 and Cox 
                 sequence 
                   
                   
               
               
                   
                 ARAH1-aptamer 
                 96 
                 TCGCACATTCCGCTTCTACCGGGGGGGTCGA 
               
               
                   
                 sequence 
                   
                 GCTGAGTGGATGCGAATCTGTGGGTGGGCCG 
               
               
                   
                   
                   
                 TAAGTCCGTGTGTGCGAA 
               
               
                   
               
            
           
         
       
     
     In some embodiments, aptamer sequences that specific bind to peanut allergen and tree nut allergen were selected through SELEX and top 100 sequences with high relative abundance were selected from each SELEX selection. Their unique sequences and full sequences including the primer sequences are listed in Table 3 (peanut) and Table 4 (tree nut). Complementary sequences specific to an aptamer may be synthesized and screened for the best binding specificity to its corresponding SPN and to the solid support. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Aptamers against peanut antigen 
               
            
           
           
               
               
               
               
            
               
                 SEQ ID NO. 
                 Unique Sequence (5′-3′) 
                 SEQ ID NO. 
                 Full sequence including primers (5′-3′) 
               
               
                   
               
               
                  97 
                 GGCCTGCGATGGATGTGTGCGTG 
                 297 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT 
               
               
                   
                 TATTAGC 
                   
                 GTGCGTGTATTAGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                  98 
                 GTACGCGCTCTGGATGTGGTTTG 
                 298 
                 TAGGGAAGAGAAGGACATATGATGTACGCGCTCTGGATG 
               
               
                   
                 TTAGTCT 
                   
                 TGGTTTGTTAGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                  99 
                 GGCCGGCGATGGATGTGGTTGTG 
                 299 
                 TAGGGAAGAGAAGGACATATGATGGCCGGCGATGGATGT 
               
               
                   
                 CTTGTTT 
                   
                 GGTTGTGCTTGTTTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 100 
                 GTGCGACCGACCCTATCAGGTGC 
                 300 
                 TAGGGAAGAGAAGGACATATGATGTGCGACCGACCCTAT 
               
               
                   
                 TCATGTA 
                   
                 CAGGTGCTCATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 101 
                 GGCCGCGTCTGGATGTGGTTTTG 
                 301 
                 TAGGGAAGAGAAGGACATATGATGGCCGCGTCTGGATGT 
               
               
                   
                 TCGCGTC 
                   
                 GGTTTTGTCGCGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 102 
                 GTCCGACGATGGATGTGGATGTA 
                 302 
                 TAGGGAAGAGAAGGACATATGATGTCCGACGATGGATGT 
               
               
                   
                 TTGGTCT 
                   
                 GGATGTATTGGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 103 
                 GGCCTGCGATGGATGTGGGCTAG 
                 303 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT 
               
               
                   
                 TATGGGC 
                   
                 GGGCTAGTATGGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 104 
                 GTTCCGCCGATGGATGTGGGTGT 
                 304 
                 TAGGGAAGAGAAGGACATATGATGTTCCGCCGATGGATG 
               
               
                   
                 AGTTGTC 
                   
                 TGGGTGTAGTTGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 105 
                 GGCATTCGATGGATGGGGTGGTG 
                 305 
                 TAGGGAAGAGAAGGACATATGATGGCATTCGATGGATGG 
               
               
                   
                 TTAGTCT 
                   
                 GGTGGTGTTAGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 106 
                 GTCCGCAGCTGGATGGGGGAGTG 
                 306 
                 TAGGGAAGAGAAGGACATATGATGTCCGCAGCTGGATGG 
               
               
                   
                 TCTGGTT 
                   
                 GGGAGTGTCTGGTTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 107 
                 GTCAGTCGATGGATGTGGGTTGT 
                 307 
                 TAGGGAAGAGAAGGACATATGATGTCAGTCGATGGATGT 
               
               
                   
                 GCTCGTC 
                   
                 GGGTTGTGCTCGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 108 
                 GTACGACGCGGACCTTCAAGTAG 
                 308 
                 TAGGGAAGAGAAGGACATATGATGTACGACGCGGACCTT 
               
               
                   
                 GCGTGTA 
                   
                 CAAGTAGGCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 109 
                 GGCCTCGAAGCCCTTCAAGCGGT 
                 309 
                 TAGGGAAGAGAAGGACATATGATGGCCTCGAAGCCCTTC 
               
               
                   
                 TCATGGA 
                   
                 AAGCGGTTCATGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 110 
                 GGCCGATCTGGATGGGGGCTCGG 
                 310 
                 TAGGGAAGAGAAGGACATATGATGGCCGATCTGGATGGG 
               
               
                   
                 GCGAGTC 
                   
                 GGCTCGGGCGAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 111 
                 GGCCTGCGATGGATGAGGTGCTG 
                 311 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGA 
               
               
                   
                 CACTAGT 
                   
                 GGTGCTGCACTAGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 112 
                 GTACGCGCTCTGGATGTGGTTGG 
                 312 
                 TAGGGAAGAGAAGGACATATGATGTACGCGCTCTGGATG 
               
               
                   
                 TTAGTCT 
                   
                 TGGTTGGTTAGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 113 
                 GGCCGCCGATGGATGTTGGCATT 
                 313 
                 TAGGGAAGAGAAGGACATATGATGGCCGCCGATGGATGT 
               
               
                   
                 GCTGGTC 
                   
                 TGGCATTGCTGGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 114 
                 GGCCGATCTGGATGGGGTATGTG 
                 314 
                 TAGGGAAGAGAAGGACATATGATGGCCGATCTGGATGGG 
               
               
                   
                 TCTAGGC 
                   
                 GTATGTGTCTAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 115 
                 GTCCGTCGATGGATGGGGTTGGG 
                 315 
                 TAGGGAAGAGAAGGACATATGATGTCCGTCGATGGATGG 
               
               
                   
                 TTCAGTC 
                   
                 GGTTGGGTTCAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 116 
                 GTGCTTGGATCCCTATCAGGTGG 
                 316 
                 TAGGGAAGAGAAGGACATATGATGTGCTTGGATCCCTAT 
               
               
                   
                 ACGTGTA 
                   
                 CAGGTGGACGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 117 
                 GTCGTTCGATGGATGTGTGCTGT 
                 317 
                 TAGGGAAGAGAAGGACATATGATGTCGTTCGATGGATGT 
               
               
                   
                 ATGAGTC 
                   
                 GTGCTGTATGAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 118 
                 GTCCGACGATGGGTGGGGGAATG 
                 318 
                 TAGGGAAGAGAAGGACATATGATGTCCGACGATGGGTGG 
               
               
                   
                 CGACGGC 
                   
                 GGGAATGCGACGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 119 
                 GTGCGCCGATGGGTGTGTGTCGT 
                 319 
                 TAGGGAAGAGAAGGACATATGATGTGCGCCGATGGGTGT 
               
               
                   
                 GGCTGGT 
                   
                 GTGTCGTGGCTGGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 120 
                 GTCCTGATCTGGGTGTGGGGGTG 
                 320 
                 TAGGGAAGAGAAGGACATATGATGTCCTGATCTGGGTGT 
               
               
                   
                 TGCAGGC 
                   
                 GGGGGTGTGCAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 121 
                 GGCCTGACTGGGTGTGGGTAGTT 
                 321 
                 TAGGGAAGAGAAGGACATATGATGGCCTGACTGGGTGTG 
               
               
                   
                 ACTTGGC 
                   
                 GGTAGTTACTTGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 122 
                 GGACGCATCGCCCCTTCGAGTGG 
                 322 
                 TAGGGAAGAGAAGGACATATGATGGACGCATCGCCCCTT 
               
               
                   
                 ACAGGTA 
                   
                 CGAGTGGACAGGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 123 
                 GGCCTGACTGGGTGTGGTTAGGA 
                 323 
                 TAGGGAAGAGAAGGACATATGATGGCCTGACTGGGTGTG 
               
               
                   
                 ACGCGTC 
                   
                 GTTAGGAACGCGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 124 
                 GTCATGCGATGGATGGGGGCTGG 
                 324 
                 TAGGGAAGAGAAGGACATATGATGTCATGCGATGGATGG 
               
               
                   
                 CAGTCGT 
                   
                 GGGCTGGCAGTCGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 125 
                 AATGTGGCGGGATCTGGATGTGT 
                 325 
                 TAGGGAAGAGAAGGACATATGATAATGTGGCGGGATCTG 
               
               
                   
                 GCATGTA 
                   
                 GATGTGTGCATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 126 
                 GGCCTGGTCTGGATGGGGGCGGG 
                 326 
                 TAGGGAAGAGAAGGACATATGATGGCCTGGTCTGGATGG 
               
               
                   
                 TATAGGC 
                   
                 GGGCGGGTATAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 127 
                 GGCCTGGGATGGATGTGGTGTAT 
                 327 
                 TAGGGAAGAGAAGGACATATGATGGCCTGGGATGGATGT 
               
               
                   
                 TAGGCTG 
                   
                 GGTGTATTAGGCTGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 128 
                 GCCCGACTCTGGATGGGGGAATG 
                 328 
                 TAGGGAAGAGAAGGACATATGATGCCCGACTCTGGATGG 
               
               
                   
                 CGCAGTC 
                   
                 GGGAATGCGCAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 129 
                 GTCGTGCTCTGGGTGGGGTTGTG 
                 329 
                 TAGGGAAGAGAAGGACATATGATGTCGTGCTCTGGGTGG 
               
               
                   
                 TAGTAGT 
                   
                 GGTTGTGTAGTAGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 130 
                 GTCATTCGCTGGATGTGTTGATG 
                 330 
                 TAGGGAAGAGAAGGACATATGATGTCATTCGCTGGATGT 
               
               
                   
                 TCTGGTC 
                   
                 GTTGATGTCTGGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 131 
                 GGCCGGCGATGGATGTGTATGTG 
                 331 
                 TAGGGAAGAGAAGGACATATGATGGCCGGCGATGGATGT 
               
               
                   
                 GTAGTCG 
                   
                 GTATGTGGTAGTCGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 132 
                 GGACGCGGCTGGATGGGGGCTTG 
                 332 
                 TAGGGAAGAGAAGGACATATGATGGACGCGGCTGGATGG 
               
               
                   
                 CACTGTC 
                   
                 GGGCTTGCACTGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 133 
                 GGCCGGCGATGGATGTGTGCGTG 
                 333 
                 TAGGGAAGAGAAGGACATATGATGGCCGGCGATGGATGT 
               
               
                   
                 TATTAGC 
                   
                 GTGCGTGTATTAGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 134 
                 GGCCGCCGATGGGTGTGGAGGTG 
                 334 
                 TAGGGAAGAGAAGGACATATGATGGCCGCCGATGGGTGT 
               
               
                   
                 TACTTGT 
                   
                 GGAGGTGTACTTGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 135 
                 GGCCTGCGATGGATGTGGTTGTG 
                 335 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT 
               
               
                   
                 CTTGTTT 
                   
                 GGTTGTGCTTGTTTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 136 
                 GGCCGACGATGGCGTAGCGTCTG 
                 336 
                 TAGGGAAGAGAAGGACATATGATGGCCGACGATGGCGTA 
               
               
                   
                 TACTGTT 
                   
                 GCGTCTGTACTGTTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 137 
                 GGCCGTGTCTGGGTGGGGGTATG 
                 337 
                 TAGGGAAGAGAAGGACATATGATGGCCGTGTCTGGGTGG 
               
               
                   
                 CACTGTC 
                   
                 GGGTATGCACTGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 138 
                 GGCCGGCGATGGATGTGAAATGG 
                 338 
                 TAGGGAAGAGAAGGACATATGATGGCCGGCGATGGATGT 
               
               
                   
                 CTTGTCT 
                   
                 GAAATGGCTTGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 139 
                 GGCCGCGTCTGGGTGTTGGTTGT 
                 339 
                 TAGGGAAGAGAAGGACATATGATGGCCGCGTCTGGGTGT 
               
               
                   
                 GTTAGGC 
                   
                 TGGTTGTGTTAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 140 
                 GGACAGCGATGGATGTGGAAATG 
                 340 
                 TAGGGAAGAGAAGGACATATGATGGACAGCGATGGATGT 
               
               
                   
                 CGACGTC 
                   
                 GGAAATGCGACGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 141 
                 GGACTGCGCGTGACCTATCAATG 
                 341 
                 TAGGGAAGAGAAGGACATATGATGGACTGCGCGTGACCT 
               
               
                   
                 GCATGTA 
                   
                 ATCAATGGCATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 142 
                 GGCCAGCTCTGGATGAGGTCTGT 
                 342 
                 TAGGGAAGAGAAGGACATATGATGGCCAGCTCTGGATGA 
               
               
                   
                 GCGAGGC 
                   
                 GGTCTGTGCGAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 143 
                 GTCCGACCGCACCCTTCGAGGGG 
                 343 
                 TAGGGAAGAGAAGGACATATGATGTCCGACCGCACCCTT 
               
               
                   
                 ACATGGA 
                   
                 CGAGGGGACATGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 144 
                 GGCCAATGCGCCCCTTCATGTTG 
                 344 
                 TAGGGAAGAGAAGGACATATGATGGCCAATGCGCCCCTT 
               
               
                   
                 TCGTGTA 
                   
                 CATGTTGTCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 145 
                 GGCCGGCTATACCCTACACGTGA 
                 345 
                 TAGGGAAGAGAAGGACATATGATGGCCGGCTATACCCTA 
               
               
                   
                 TCAGGTA 
                   
                 CACGTGATCAGGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 146 
                 GTGCGCACGATGGATGTGGATGG 
                 346 
                 TAGGGAAGAGAAGGACATATGATGTGCGCACGATGGATG 
               
               
                   
                 CCAGTCT 
                   
                 TGGATGGCCAGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 147 
                 GGCATGCGATGGATGGGGGCTGG 
                 347 
                 TAGGGAAGAGAAGGACATATGATGGCATGCGATGGATGG 
               
               
                   
                 GGCAGTC 
                   
                 GGGCTGGGGCAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 148 
                 GTCATGCGCTGGATGTGGGTTGT 
                 348 
                 TAGGGAAGAGAAGGACATATGATGTCATGCGCTGGATGT 
               
               
                   
                 TATAGGC 
                   
                 GGGTTGTTATAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 149 
                 GTGCGCCTCTGGATGGGGTTTGT 
                 349 
                 TAGGGAAGAGAAGGACATATGATGTGCGCCTCTGGATGG 
               
               
                   
                 CGACGGC 
                   
                 GGTTTGTCGACGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 150 
                 GGCCAGCTCTGGATGTGGCATTG 
                 350 
                 TAGGGAAGAGAAGGACATATGATGGCCAGCTCTGGATGT 
               
               
                   
                 TCTGGTC 
                   
                 GGCATTGTCTGGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 151 
                 GGCCTGAGCTGGCATTGCGCTGG 
                 351 
                 TAGGGAAGAGAAGGACATATGATGGCCTGAGCTGGCATT 
               
               
                   
                 ACATGTA 
                   
                 GCGCTGGACATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 152 
                 GGCCGCCGATGGGTGTGGGATCG 
                 352 
                 TAGGGAAGAGAAGGACATATGATGGCCGCCGATGGGTGT 
               
               
                   
                 TGCGGGC 
                   
                 GGGATCGTGCGGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 153 
                 GGCCGACGCTGGGTGGGGGCGTT 
                 353 
                 TAGGGAAGAGAAGGACATATGATGGCCGACGCTGGGTGG 
               
               
                   
                 GACTACGT 
                   
                 GGGCGTTGACTAGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 154 
                 GGACTACGCTGGATGGGGACATT 
                 354 
                 TAGGGAAGAGAAGGACATATGATGGACTACGCTGGATGG 
               
               
                   
                 GCTAGTT 
                   
                 GGACATTGCTAGTTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 155 
                 GGCATGCGATGGGTGTGTTCTGG 
                 355 
                 TAGGGAAGAGAAGGACATATGATGGCATGCGATGGGTGT 
               
               
                   
                 CGACGGC 
                   
                 GTTCTGGCGACGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 156 
                 GGACTACGCTGGATGTGGGTTGG 
                 356 
                 TAGGGAAGAGAAGGACATATGATGGACTACGCTGGATGT 
               
               
                   
                 GGTAGTC 
                   
                 GGGTTGGGGTAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 157 
                 GGCCGAGCTTACCTGTCAAGTGC 
                 357 
                 TAGGGAAGAGAAGGACATATGATGGCCGAGCTTACCTGT 
               
               
                   
                 GAGTGTA 
                   
                 CAAGTGCGAGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 158 
                 GTGCTTCGATGGATGTGGGCGTT 
                 358 
                 TAGGGAAGAGAAGGACATATGATGTGCTTCGATGGATGT 
               
               
                   
                 GCAAGTC 
                   
                 GGGCGTTGCAAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 159 
                 GTCCGCGGCTGGGTGTGGGAAGT 
                 359 
                 TAGGGAAGAGAAGGACATATGATGTCCGCGGCTGGGTGT 
               
               
                   
                 ACTCGGC 
                   
                 GGGAAGTACTCGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 160 
                 GTCCGCGCGATGGATGAGGACGT 
                 360 
                 TAGGGAAGAGAAGGACATATGATGTCCGCGCGATGGATG 
               
               
                   
                 GAGCTAG 
                   
                 AGGACGTGAGCTAGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 161 
                 GGCCGAGTCGTCACTTCAATTGG 
                 361 
                 TAGGGAAGAGAAGGACATATGATGGCCGAGTCGTCACTT 
               
               
                   
                 GCGTGGA 
                   
                 CAATTGGGCGTGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 162 
                 GTCGGTCGGAGGGATGGTCTCGT 
                 362 
                 TAGGGAAGAGAAGGACATATGATGTCGGTCGGAGGGATG 
               
               
                   
                 TGACGGC 
                   
                 GTCTCGTTGACGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 163 
                 GGCCACACGCTGCCCTAAAGTGT 
                 363 
                 TAGGGAAGAGAAGGACATATGATGGCCACACGCTGCCCT 
               
               
                   
                 TCGTGTA 
                   
                 AAAGTGTTCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 164 
                 GGCCTGCGATGGATGTGTGCGTG 
                 364 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT 
               
               
                   
                 TATTAGT 
                   
                 GTGCGTGTATTAGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 165 
                 GTGCGCTGTCTTGCCTACAAGTG 
                 365 
                 TAGGGAAGAGAAGGACATATGATGTGCGCTGTCTTGCCT 
               
               
                   
                 TCGTGTA 
                   
                 ACAAGTGTCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 166 
                 GGCCGGAACTGGATGGGGGCATT 
                 366 
                 TAGGGAAGAGAAGGACATATGATGGCCGAACTGGATGGG 
               
               
                   
                 ACTGCGGC 
                   
                 GGCATTACTGCGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 167 
                 GGCCGAGCTGGATGGGGTATTGG 
                 367 
                 TAGGGAAGAGAAGGACATATGATGGCCGAGCTGGATGGG 
               
               
                   
                 ATCTAGT 
                   
                 GTATTGGATCTAGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 168 
                 TGGCCGCACTCTGGGTGTGGTGG 
                 368 
                 TAGGGAAGAGAAGGACATATGATTGGCCGCACTCTGGGT 
               
               
                   
                 ACTTGGC 
                   
                 GTGGTGGACTTGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 169 
                 GGCCGGTCTGGATGGGGGCGTAC 
                 369 
                 TAGGGAAGAGAAGGACATATGATGGCCGGTCTGGATGGG 
               
               
                   
                 GTCGTCG 
                   
                 GGCGTACGTCGTCGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 170 
                 GGCCTGCTATGGGTGGGGGCTCG 
                 370 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCTATGGGTGG 
               
               
                   
                 TACTGGC 
                   
                 GGGCTCGTACTGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 171 
                 GTACGCCCGATGGATGTGGATTG 
                 371 
                 TAGGGAAGAGAAGGACATATGATGTACGCCCGATGGATG 
               
               
                   
                 TACTGTT 
                   
                 TGGATTGTACTGTTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 172 
                 GGCCTGCGATGGATGTGGGCCAG 
                 372 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT 
               
               
                   
                 TACGCGC 
                   
                 GGGCCAGTACGCGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 173 
                 GACCTGCGATGGGTGTGGCTCTG 
                 373 
                 TAGGGAAGAGAAGGACATATGATGACCTGCGATGGGTGT 
               
               
                   
                 TCAGTCT 
                   
                 GGCTCTGTCAGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 174 
                 GTCCGAACTGGATGTGGTATTGG 
                 374 
                 TAGGGAAGAGAAGGACATATGATGTCCGAACTGGATGTG 
               
               
                   
                 CCTGTCT 
                   
                 GTATTGGCCTGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 175 
                 GTTCTTGTCTGGATGGGGTTGAT 
                 375 
                 TAGGGAAGAGAAGGACATATGATGTTCTTGTCTGGATGG 
               
               
                   
                 GTCGGTC 
                   
                 GGTTGATGTCGGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 176 
                 GTACTCGATGGGTGTGGGTAGGC 
                 376 
                 TAGGGAAGAGAAGGACATATGATGTACTCGATGGGTGTG 
               
               
                   
                 GCGAGTC 
                   
                 GGTAGGCGCGAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 177 
                 GTCCTCGATGGGTGTGAGATATG 
                 377 
                 TAGGGAAGAGAAGGACATATGATGTCCTCGATGGGTGTG 
               
               
                   
                 TGCTAGC 
                   
                 AGATATGTGCTAGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 178 
                 GGCCGTGCTCTGGGTGTGGGCCT 
                 378 
                 TAGGGAAGAGAAGGACATATGATGGCCGTGCTCTGGGTG 
               
               
                   
                 GCTGGGC 
                   
                 TGGGCCTGCTGGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 179 
                 GTTCGTATCTGGGGTGTGGTGTG 
                 379 
                 TAGGGAAGAGAAGGACATATGATGTTCGTATCTGGGTGT 
               
               
                   
                 TCTGGGCT 
                   
                 GGTGTGTCTGGGCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 180 
                 GGCCGAGTCTGGATGTGTGTCGT 
                 380 
                 TAGGGAAGAGAAGGACATATGATGGCCGAGTCTGGATGT 
               
               
                   
                 ACGTATC 
                   
                 GTGTCGTACGTATCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 181 
                 GGCGTTCACTGGGTGTGGATGTG 
                 381 
                 TAGGGAAGAGAAGGACATATGATGGCGTTCACTGGGTGT 
               
               
                   
                 TGCGGTC 
                   
                 GGATGTGTGCGGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 182 
                 GTCATGCGCTGGGTGTGGCCTGT 
                 382 
                 TAGGGAAGAGAAGGACATATGATGTCATGCGCTGGGTGT 
               
               
                   
                 TGTAGGC 
                   
                 GGCCTGTTGTAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 183 
                 GCTCCGTACGATGGCTGTGCTGG 
                 383 
                 TAGGGAAGAGAAGGACATATGATGCTCCGTACGATGGCT 
               
               
                   
                 TGATGTA 
                   
                 GTGCTGGTGATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 184 
                 GGACTGCGATGGGTGGGGTTATG 
                 384 
                 TAGGGAAGAGAAGGACATATGATGGACTGCGATGGGTGG 
               
               
                   
                 TGCCAGC 
                   
                 GGTTATGTGCCAGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 185 
                 GTGCGATCGTACCTATCAATGGT 
                 385 
                 TAGGGAAGAGAAGGACATATGATGTGCGATCGTACCTAT 
               
               
                   
                 CATCGTA 
                   
                 CAATGGTCATCGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 186 
                 GTTCGTCGAATGGATGTGAGCAT 
                 386 
                 TAGGGAAGAGAAGGACATATGATGTTCGTCGAATGGATG 
               
               
                   
                 GTCTGTC 
                   
                 TGAGCATGTCTGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 187 
                 GGCCGTACGATGGATGTGGGATG 
                 387 
                 TAGGGAAGAGAAGGACATATGATGGCCGTACGATGGATG 
               
               
                   
                 GTCTAGC 
                   
                 TGGGATGGTCTAGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 188 
                 GTCATTAACTGGATGTGGGACTG 
                 388 
                 TAGGGAAGAGAAGGACATATGATGTCATTAACTGGATGT 
               
               
                   
                 TCGGTCT 
                   
                 GGGACTGTCGGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 189 
                 GGACGATCTGGGTGTGGACAGGG 
                 389 
                 TAGGGAAGAGAAGGACATATGATGGACGATCTGGGTGTG 
               
               
                   
                 ATGAGGC 
                   
                 GACAGGGATGAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 190 
                 GGCATGCGATGGATGTGGTGTAC 
                 390 
                 TAGGGAAGAGAAGGACATATGATGGCATGCGATGGATGT 
               
               
                   
                 CCAGTCC 
                   
                 GGTGTACCCAGTCCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 191 
                 GGCCGCGATGGATGTGGAAAGGT 
                 391 
                 TAGGGAAGAGAAGGACATATGATGGCCGCGATGGATGTG 
               
               
                   
                 CTAGTCA 
                   
                 GAAAGGTCTAGTCATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 192 
                 GGCCGACCTGGATGTGAGCATGC 
                 392 
                 TAGGGAAGAGAAGGACATATGATGGCCGACCTGGATGTG 
               
               
                   
                 ATCTAGT 
                   
                 AGCATGCATCTAGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 193 
                 GTACGAGCGGACCGATCAAGTGC 
                 393 
                 TAGGGAAGAGAAGGACATATGATGTACGAGCGGACCGAT 
               
               
                   
                 GTCTGTA 
                   
                 CAAGTGCGTCTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 194 
                 GTACTGCACTGCCCTACACGTGG 
                 394 
                 TAGGGAAGAGAAGGACATATGATGTACTGCACTGCCCTA 
               
               
                   
                 GAATGGA 
                   
                 CACGTGGGAATGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 195 
                 GTCATTCGATGGATGTGGCGTGT 
                 395 
                 TAGGGAAGAGAAGGACATATGATGTCATTCGATGGATGT 
               
               
                   
                 GCGTCAT 
                   
                 GGCGTGTGCGTCATTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 196 
                 GGCGTACGATGGATTGCGTTGTG 
                 396 
                 TAGGGAAGAGAAGGACATATGATGGCGTACGATGGATGT 
               
               
                   
                 TCTGTC 
                   
                 GCGTTGTGTCTGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 197 
                 GGCCTGCGATGGATGTGTGCGTG 
                 397 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT 
               
               
                   
                 TATTAGC 
                   
                 GTGCGTGTATTAGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 198 
                 GTCATTCCGCATCCTACACGTGG 
                 398 
                 TAGGGAAGAGAAGGACATATGATGTCATTCCGCATCCTA 
               
               
                   
                 GCACGTA 
                   
                 CACGTGGGCACGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 199 
                 GGCCAATGCGCCCCTTCATGTTG 
                 399 
                 TAGGGAAGAGAAGGACATATGATGGCCAATGCGCCCCTT 
               
               
                   
                 TCGTGTA 
                   
                 CATGTTGTCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 200 
                 GGCCTGCGATGGATAGGTGCTGC 
                 400 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGA 
               
               
                   
                 ACTAGT 
                   
                 GGTGCTGCACTAGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 201 
                 GTCAGTCGATGGATGTGGGTTGT 
                 401 
                 TAGGGAAGAGAAGGACATATGATGTCAGTCGATGGATGT 
               
               
                   
                 GCTCGTC 
                   
                 GGGTTGTGCTCGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 202 
                 GTCGTGACTGGCTAGCTGGACAT 
                 402 
                 TAGGGAAGAGAAGGACATATGATGTCGTGACTGGCTAGCT 
               
               
                   
                 GCACTGC 
                   
                 GGACATGCACTGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 203 
                 GGCCTGCGATGGATGTGGGCTAG 
                 403 
                 TAGTGGAAGAGAAGGACATATGATGGCCTGCGATGGATG 
               
               
                   
                 TATGGGC 
                   
                 TGGGCTAGTATGGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 204 
                 GTGCATCGATGGCGTATGCTGGT 
                 404 
                 TAGGGAAGAGAAGGACATATGATGTGCATCGATGGCGTAT 
               
               
                   
                 GATGTGC 
                   
                 GCTGGTGATGTGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 205 
                 GCGACAGCGACATACGATCTGCT 
                 405 
                 TAGGGAAGAGAAGGACATATGATGCGACAGCGACATACG 
               
               
                   
                 CTGCGTC 
                   
                 ATCTGCTCTGCGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 206 
                 GTCATGCCATCCCTTCGAGTGTG 
                 406 
                 TAGGGAAGAGAAGGACATATGATGTCATGCCATCCCTTC 
               
               
                   
                 ACAGGTA 
                   
                 GAGTGTGACAGGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 207 
                 GGCCTGACTGGGTGTGGTTAGGA 
                 407 
                 TAGGGAAGAGAAGGACATATGATGGCCTGACTGGGTGTG 
               
               
                   
                 ACGCGTC 
                   
                 GTTAGGAACGCGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 208 
                 GTGCGCACGATGGATGTGGATGG 
                 408 
                 TAGGGAAGAGAAGGACATATGATGTGCGCACGATGGATG 
               
               
                   
                 CCAGTCT 
                   
                 TGGATGGCCAGTCTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 209 
                 GTGCGCCGATGGGTGTGTGTCGT 
                 409 
                 TAGGGAAGAGAAGGACATATGATGTGCGCCGATGGGTGT 
               
               
                   
                 GGCTGGT 
                   
                 GTGTCGTGGCTGGTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 210 
                 GGCATGCGATGGATGTGGTGTAC 
                 410 
                 TAGGGAAGAGAAGGACATATGATGGCATGCGATGGATGT 
               
               
                   
                 CCAGTCC 
                   
                 GGTGTACCCAGTCCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 211 
                 CCATATGGCAGTGCGATGGCTTC 
                 411 
                 TAGGGAAGAGAAGGACATATGATCCATATGGCAGTGCGA 
               
               
                   
                 GCTGGTC 
                   
                 TGGCTTCGCTGGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 212 
                 GCTCCGTACGATGGCTGTGCTGG 
                 412 
                 TAGGGAAGAGAAGGACATATGATGCTCCGTACGATGGCT 
               
               
                   
                 TGATGTA 
                   
                 GTGCTGGTGATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 213 
                 GTGCGACCGACCCTATCAGGTGC 
                 413 
                 TAGGGAAGAGAAGGACATATGATGTGCGACCGACCCTAT 
               
               
                   
                 TCATGTA 
                   
                 CAGGTGCTCATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 214 
                 GTTCCGCCGATGGATGTGGGTGT 
                 414 
                 TAGGGAAGAGAAGGACATATGATGTTCCGCCGATGGATG 
               
               
                   
                 AGTTGTC 
                   
                 TGGGTGTAGTTGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 215 
                 GTACTGCACTGCCCTACACGTGG 
                 415 
                 TAGGGAAGAGAAGGACATATGATGTACTGCACTGCCCTA 
               
               
                   
                 GAATGGA 
                   
                 CACGTGGGAATGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 216 
                 CGCACCGTCGATACGTCATGCAC 
                 416 
                 TAGGGAAGAGAAGGACATATGATCGCACCGTCGATACGTC 
               
               
                   
                 GCTGACA 
                   
                 ATGCACGCTGACATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 217 
                 GTACGCACGATGAGCGCCAAGTG 
                 417 
                 TAGGGAAGAGAAGGACATATGATGTACGCACGATGAGCG 
               
               
                   
                 ACATGGA 
                   
                 CCAAGTGACATGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 218 
                 GTCGGTCGGAGGGATGGTCTCGT 
                 418 
                 TAGGGAAGAGAAGGACATATGATGTCGGTCGGAGGGATG 
               
               
                   
                 TGACGGC 
                   
                 GTCTCGTTGACGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 219 
                 GGCATGCGATGAACGAGGCATGA 
                 419 
                 TAGGGAAGAGAAGGACATATGATGGCATGCGATGAACGA 
               
               
                   
                 TGCGTCA 
                   
                 GGCATGATGCGTCATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 220 
                 GGCGTACGATGGATGTGCGTTGT 
                 420 
                 TAGGGAAGAGAAGGACATATGATGGCGTACGATGGATGT 
               
               
                   
                 GTCTGTC 
                   
                 GCGTTGTGTCTGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 221 
                 GTCACTGTGCCTGACCGTCAAGT 
                 421 
                 TAGGGAAGAGAAGGACATATGATGTCACTGTGCCTGACC 
               
               
                   
                 TGCGGCA 
                   
                 GTCAAGTTGCGGCATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 222 
                 GGACTGCGCGTGACCTATCAATG 
                 422 
                 TAGGGAAGAGAAGGACATATGATGGACTGCGCGTGACCT 
               
               
                   
                 GCATGTA 
                   
                 ATCAATGGCATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 223 
                 GGCCTGAGCTGGCATTGCGCTGG 
                 423 
                 TAGGGAAGAGAAGGACATATGATGGCCTGAGCTGGCATT 
               
               
                   
                 ACATGTA 
                   
                 GCGCTGGACATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 224 
                 GCATTGGACGATCGTGCCCTACA 
                 424 
                 TAGGGAAGAGAAGGACATATGATGCATTGGACGATCGTG 
               
               
                   
                 CGTGGGC 
                   
                 CCCTACACGTGGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 225 
                 GGACGCATCGCCCCTTCGAGTGG 
                 425 
                 TAGGGAAGAGAAGGACATATGATGGACGCATCGCCCCTT 
               
               
                   
                 ACAGGTA 
                   
                 CGAGTGGACACGGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 226 
                 GGCCACACGCTGCCCTAAAGTGT 
                 426 
                 TAGGGAAGAGAAGGACATATGATGGCCACACGCTGCCCT 
               
               
                   
                 TCGTGTA 
                   
                 AAAGTGTTCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 227 
                 GTTCTGACTGGGTGTGGTGCTGC 
                 427 
                 TAGGGAAGAGAAGGACATATGATGTTCTGACTGGGTGTG 
               
               
                   
                 ACTGTCA 
                   
                 GTGCTGCACTGTCATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 228 
                 GGCAACGCACATCGTATCACGCA 
                 428 
                 TAGGGAAGAGAAGGACATATGATGGCAACGCACATCGTA 
               
               
                   
                 TCGGACC 
                   
                 TCACGCATCGGACCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 229 
                 GTCATGCGCGGACATTCAAGTTG 
                 429 
                 TAGGGAAGAGAAGGACATATGATGTCATGCGCGGACATT 
               
               
                   
                 GCGTGGA 
                   
                 CAAGTTGGCGTGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 230 
                 CCGTAGCGACATCAAGCGGTGGT 
                 430 
                 TAGGGAAGAGAAGGACATATGATCCGTAGCGACATCAAG 
               
               
                   
                 GTGCGTG 
                   
                 CGGTGGTGTGCGTGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 231 
                 GTACGACGCGGACCTTCAAGTAG 
                 431 
                 TAGGGAAGAGAAGGACATATGATGTACGACGCGGACCTT 
               
               
                   
                 GCGTGTA 
                   
                 CAAGTAGGCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 232 
                 GACCTGACTGTGCCTATCGAGTG 
                 432 
                 TAGGGAAGAGAAGGACATATGATGACCTGACTGTGCCTA 
               
               
                   
                 CGTGATG 
                   
                 TCGAGTGCGTGATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 233 
                 TGGCCGATCGACCCTATCAAGTG 
                 433 
                 TAGGGAAGAGAAGGACATATGATTGGCCGATCGACCCTA 
               
               
                   
                 CAGCATG 
                   
                 TCAAGTGCAGCATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 234 
                 GTTCCGAGCTGGATGTGGCCTGT 
                 434 
                 TAGGGAAGAGAAGGACATATGATGTTCCGAGCTGGATGT 
               
               
                   
                 GCTATGC 
                   
                 GGCCTGTGCTATGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 235 
                 GGCATGCGATGGATGTGTGCGTG 
                 435 
                 TAGGGAAGAGAAGGACATATGATGGCATGCGATGGATGT 
               
               
                   
                 TATTAGC 
                   
                 GTGCGTGTATTAGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 236 
                 GTACGCATCGTCCCGTCATGTGG 
                 436 
                 TAGGGAAGAGAAGGACATATGATGTACGCATCGTCCCGT 
               
               
                   
                 TTCCGTA 
                   
                 CATGTGGTTCCGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 237 
                 GGCATTGCGCGCCTAGCAAGTTG 
                 437 
                 TAGGGAAGAGAAGGACATATGATGGCATTGCGCGCCTAG 
               
               
                   
                 ACGTGTA 
                   
                 CAAGTTGACGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 238 
                 GGACGCACGCAGACCTTCAAGTC 
                 438 
                 TAGGGAAGAGAAGGACATATGATGGACGCACGCAGACCT 
               
               
                   
                 GGCCATG 
                   
                 TCAAGTCGGCCATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 239 
                 GTGCTGCATGAGCGGTGTGCGTG 
                 439 
                 TAGGGAAGAGAAGGACATATGATGTGCTGCATGAGCGGT 
               
               
                   
                 TACGACG 
                   
                 GTGCGTGTACGACGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 240 
                 GTCATGCTCGACACTATCAGGTG 
                 440 
                 TAGGGAAGAGAAGGACATATGATGTCATGCTCGCACTAT 
               
               
                   
                 TGCATGGA 
                   
                 CAGGTGTGCATGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 241 
                 GTGCGCGGCTTGCCTTCACGTGA 
                 441 
                 TAGGGAAGAGAAGGACATATGATGTGCGCGGCTTGCCTT 
               
               
                   
                 TCGTGTA 
                   
                 CACGTGATCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 242 
                 GTCATACGATGGGTGTGGTATGT 
                 442 
                 TAGGGAAGAGAAGGACATATGATGTCATACGATGGGTGT 
               
               
                   
                 GTACGTA 
                   
                 GGTATGTGTACGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 243 
                 GCATGCGTTGGACTTGTCTGGCT 
                 443 
                 TAGGGAAGAGAAGGACATATGATGCATGCGTTGGACTTG 
               
               
                   
                 GTGGGTG 
                   
                 TCTGGCTGTGGGTGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 244 
                 TGCGTCGTATGTGCGGCTCGGAT 
                 444 
                 TAGGGAAGAGAAGGACATATGATTGCGTCGTATGTGCGG 
               
               
                   
                 GTGTGTC 
                   
                 CTCGGATGTGTGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 245 
                 GGACGCAGCTTGCCTACTGGTGG 
                 445 
                 TAGGGAAGAGAAGGACATATGATGGACGCAGCTTGCCTA 
               
               
                   
                 TCACGTA 
                   
                 CTGGTGGTCACGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 246 
                 GGCCATCGATGGGTGTGGCTGTA 
                 446 
                 TAGGGAAGAGAAGGACATATGATGGCCATCGATGGGTGT 
               
               
                   
                 CTTGACA 
                   
                 GGCTGTACTTGACATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 247 
                 GCGTGTCAGCAATACGTCCTCAT 
                 447 
                 TAGGGAAGAGAAGGACATATGATGCGTGTCAGCAATACG 
               
               
                   
                 CTGCCCG 
                   
                 TCCTCATCTGCCCGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 248 
                 GTGCGATCGTACCTATCAATGGT 
                 448 
                 TAGGGAAGAGAAGGACATATGATGTGCGATCGTACCTAT 
               
               
                   
                 CATCGTA 
                   
                 CAATGGTCATCGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 249 
                 GGCATGCGATGGATGGGGGCTGG 
                 449 
                 TAGGGAAGAGAAGGACATATGATGGCATGCGATGGATGG 
               
               
                   
                 GGCAGTC 
                   
                 GGGCTGGGGCAGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 250 
                 GGCCGACGATGGCGTAGCGTCTG 
                 450 
                 TAGGGAAGAGAAGGACATATGATGGCCGACGATGGCGTA 
               
               
                   
                 TACTGTT 
                   
                 GCGTCTGTACTGTTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 251 
                 TGGCCGATCATCCCTCAAGTTGG 
                 451 
                 TAGGGAAGAGAAGGACATATGATTGGCCGATCATCCCTC 
               
               
                   
                 CGTGTGC 
                   
                 AAGTTGGCGTGTGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 252 
                 GGCCGTACGATGGATGTGGGATG 
                 452 
                 TAGGGAAGAGAAGGACATATGATGGCCGTACGATGGATG 
               
               
                   
                 GTCTAGC 
                   
                 TGGGATGGTCTAGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 253 
                 GGCACAAACGGACCGACAAGTGC 
                 453 
                 TAGGGAAGAGAAGGACATATGATGGCACAAACGGACCGA 
               
               
                   
                 GCATGGA 
                   
                 CAAGTGCGCATGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 254 
                 GTACGGAACGGAACAACAAGGGC 
                 454 
                 TAGGGAAGAGAAGGACATATGATGTACGGAACGGAACAA 
               
               
                   
                 AGGCATG 
                   
                 CAAGGGCAGGCATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 255 
                 GGACGCACATCCCGTTCATGTGT 
                 455 
                 TAGGGAAGAGAAGGACATATGATGGACGCACATCCCGTT 
               
               
                   
                 GCATGTA 
                   
                 CATGTGTGCATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 256 
                 GGCCAAGCTGGATGTGTTCAGGT 
                 456 
                 TAGGGAAGAGAAGGACATATGATGGCCAAGCTGGATGTG 
               
               
                   
                 CACGACG 
                   
                 TTCAGGTCACGACGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 257 
                 GGCCTGCGATGGATGTGTGCGTG 
                 457 
                 TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT 
               
               
                   
                 TA 
                   
                 GTGCGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 258 
                 CGTGCGCGAGACATGTCCATCGG 
                 458 
                 TAGGGAAGAGAAGGACATATGATCGTGCGCGAGACATGG 
               
               
                   
                 TTCGTG 
                   
                 TCCATCGGTTCGTGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 259 
                 GTCATGCGCTGGGTGTGGCCTGT 
                 459 
                 TAGGGAAGAGAAGGACATATGATGTCATGCGCTGGGTGT 
               
               
                   
                 TGTAGGC 
                   
                 GGCCTGTTGTAGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 260 
                 GTCGCGATGAGCTAGCATGTGCG 
                 460 
                 TAGGGAAGAGAAGGACATATGATGTCGCGATGAGCTAGC 
               
               
                   
                 TTGTGTA 
                   
                 ATGTGCGTTGTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 261 
                 GTGCTACGATGGCTGTGGGCGTG 
                 461 
                 TAGGGAAGAGAAGGACATATGATGTGCTACGATGGCTGT 
               
               
                   
                 ATGCGTA 
                   
                 GGGCGTGATGCGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 262 
                 GACGCTGCGTTCCCTATCATGTG 
                 462 
                 TAGGGAAGAGAAGGACATATGATGACGCTGCGTTCCCTA 
               
               
                   
                 CGGCATG 
                   
                 TCATGTGCGGCATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 263 
                 GTTCGCGCGACACCTATCAATGT 
                 463 
                 TAGGAAGAGAAGGACATATGATGTCCGCGCGACACCTAT 
               
               
                   
                 GGACGTG 
                   
                 CAATGTGGACGTGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 264 
                 GCACGACTCGCACCCTATCATGA 
                 464 
                 TAGGGAAGAGAAGGACATATGATGCACGACTCGCACCCT 
               
               
                   
                 GGCCATG 
                   
                 ATCATGAGGCCATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 265 
                 GTCATGAAACGAGCCTACACGTG 
                 465 
                 TAGGGAAGAGAAGGACATATGATGTCATGAAACGAGCCT 
               
               
                   
                 GTGCATG 
                   
                 ACACGTGGTGCATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 266 
                 GGACGCGATGGGCGTGGGTATGC 
                 466 
                 TAGGGAAGAGAAGGACATATGATGGACGCGATGGGCGTG 
               
               
                   
                 ACTTGGC 
                   
                 GGTATGCACTTGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 267 
                 GTACTGCGATGGCTTAGCAAAGT 
                 467 
                 TAGGGAAGAGAAGGACATATGATGTACTGCGATGGCTTA 
               
               
                   
                 GCGACATG 
                   
                 GCAAAGTGCGACATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 268 
                 TGCCCTGGCAATGCGATGTTCGA 
                 468 
                 TAGGGAAGAGAAGGACATATGATTGCCCTGGCAATGCGA 
               
               
                   
                 TGCGACC 
                   
                 TGTTCGATGCGACCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 269 
                 GTACGCGACGTCCCTGAGAGTGT 
                 469 
                 TAGGGAAGAGAAGGACATATGATGTACGCGACGTCCCTG 
               
               
                   
                 GCAGGTA 
                   
                 AGAGTGTGCAGGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 270 
                 GTGCGCCGATATCCCTTCACAGT 
                 470 
                 TAGGGAAGAGAAGGACATATGATGTGCGCCGATATCCCT 
               
               
                   
                 TGGCATG 
                   
                 TCACAGTTGGCATGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 271 
                 GGCCGACGATGGATGGGAGGCAT 
                 471 
                 TAGGGAAGAGAAGGACATATGATGGCCGACGATGGATGG 
               
               
                   
                 GACTGGC 
                   
                 GAGGCATGACTGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 272 
                 GTACGCGCTGGTCCCGTATCATG 
                 472 
                 TAGGGAAGAGAAGGACATATGATGTACGCGCTGGTCCCG 
               
               
                   
                 TGCGTCA 
                   
                 TATCATGTGCGTCATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 273 
                 GGCCTCGATGGATGTGGTGGTGC 
                 473 
                 TAGGGAAGAGAAGGACATATGATGGCCTCGATGGATGTG 
               
               
                   
                 TGTCA 
                   
                 GTGGTGCTGTCATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 274 
                 GGCCGAACTGGATGGGGGCATTA 
                 474 
                 TAGGGAAGAGAAGGACATATGATGGCCGAACTGGATGGG 
               
               
                   
                 CTGCGGC 
                   
                 GGCATTACTGCGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 275 
                 GTCACGGAACGAAGCCTATCAAG 
                 475 
                 TAGGGAAGAGAAGGACATATGATGTCACGGAACGAAGCC 
               
               
                   
                 TGCGACA 
                   
                 TATCAAGTGCGACATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 276 
                 GGCACGGAGGATGGACGTTCTGC 
                 476 
                 TAGGGAAGAGAAGGACATATGATGGCACGGAGGATGGAC 
               
               
                   
                 CTTGGTC 
                   
                 GTTCTGCCTTGGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 277 
                 GCAACTGCACGCATTGGACCGCA 
                 477 
                 TAGGGAAGAGAAGGACATATGATGCAACTGCACGCATTG 
               
               
                   
                 CGTCACA 
                   
                 GACCGCACGTCACATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 278 
                 GTGCTGAGCTGGGAGTGGTGGTG 
                 478 
                 TAGGGAAGAGAAGGACATATGATGTGCTGAGCTGGGAGT 
               
               
                   
                 CTTTGGC 
                   
                 GGTGGTGCTTTGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 279 
                 GTCTGCCGATGGATTGGTGTACG 
                 479 
                 TAGGGAAGAGAAGGACATATGATGTCTGCCGATGGATGT 
               
               
                   
                 CAGACG 
                   
                 GGTGTACGCAGACGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 280 
                 GTACACGATGCACCTTCAAGTTG 
                 480 
                 TAGGGAAGAGAAGGACATATGATGTACACGATGCACCTT 
               
               
                   
                 TGATGTA 
                   
                 CAAGTTGTGATGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 281 
                 GGACGCGTCGACCTTCAAGTGTG 
                 481 
                 TAGGGAAGAGAAGGACATATGATGGACGCGTCGACCTTC 
               
               
                   
                 CCGTGGA 
                   
                 AAGTGTGCCGTGGATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 282 
                 GTCATTCGCTGGATGTGTTGATG 
                 482 
                 TAGGGAAGAGAAGGACATATGATGTCATTCGCTGGATGT 
               
               
                   
                 TCTGGTC 
                   
                 GTTGATGTCTGGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 283 
                 CGCATGGGACGTACTGACCGGAT 
                 483 
                 TAGGGAAGAGAAGGACATATGATCGCATGGGACGTACTG 
               
               
                   
                 CGTGTCA 
                   
                 ACCGGATCGTGTCATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 284 
                 GGCCACGATGGATGAGGACATGA 
                 484 
                 TAGGGAAGAGAAGGACATATGATGGCCACGATGGATGAG 
               
               
                   
                 CTGGTTG 
                   
                 GACATGACTGGTTGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 285 
                 GTGCGACACGTGTTCCCGTTCAA 
                 485 
                 TAGGGAAGAGAAGGACATATGATGTGCGACACGTGTTCC 
               
               
                   
                 GTTGGGC 
                   
                 CGTTCAAGTTGGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 286 
                 CACGACAGCGTTAGCAGGCCATG 
                 486 
                 TAGGGAAGAGAAGGACATATGATCACGACAGCGTTAGCA 
               
               
                   
                 CGACACG 
                   
                 GGCCATGCGACACGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 287 
                 GTCGTGCGTGCCCTATCAAGTCG 
                 487 
                 TAGGGAAGAGAAGGACATATGATGTCGTGCGTGCCCTAT 
               
               
                   
                 GTCTGTA 
                   
                 CAAGTCGGTCTGTATTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 288 
                 GGCATGCGATGGGTGTGTTCTGG 
                 488 
                 TAGGGAAGAGAAGGACATATGATGGCATGCGATGGGTGT 
               
               
                   
                 CGACGGC 
                   
                 GTTCTGGCGACGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 289 
                 GTCTGAGCGCAACCTCGTGGACT 
                 489 
                 TAGGGAAGAGAAGGACATATGATGTCTGAGCGCAACCTC 
               
               
                   
                 GTGCGTG 
                   
                 GTGGACTGTGCGTGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 290 
                 GGCCGCGTCTGGATGTGGTTTTG 
                 490 
                 TAGGGAAGAGAAGGACATATGATGGCCGCGTCTGGATGT 
               
               
                   
                 TCGCGTC 
                   
                 GGTTTTGTCGCGTCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 291 
                 CCGTGTTGCGTGTCCAGTCTCGT 
                 491 
                 TAGGGAAGAGAAGGACATATGATCCGTGTTGCGTGTCCA 
               
               
                   
                 TGCGCG 
                   
                 GTCTCGTTGCGCGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 292 
                 GGCCGGCGATGGATGTGGTTGTG 
                 492 
                 TAGGGAAGAGAAGGACATATGATGGCCGGCGATGGATGT 
               
               
                   
                 CTTGTTT 
                   
                 GGTTGTGCTTGTTTTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 293 
                 GTGCGATACATCCCAACCTCCCG 
                 493 
                 TAGGGAAGAGAAGGACATATGATGTGCGATACATCCCAAC 
               
               
                   
                 TGTGGC 
                   
                 CCTCCCGTGTGGCTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 294 
                 GCCGCAACGACTGAGGGGTGTAT 
                 494 
                 TAGGGAAGAGAAGGACATATGATGCCGCAACGACTGAGG 
               
               
                   
                 GTACGCG 
                   
                 GGTGTATGTACGCGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 295 
                 GGACGCGGATGAGCTTCGAGTGA 
                 495 
                 TAGGGAAGAGAAGGACATATGATGGACGCGGATGAGCTT 
               
               
                   
                 CGTGTAC 
                   
                 CGAGTGACGTGTACTTGACTAGTACATGACCACTTGA 
               
               
                   
               
               
                 296 
                 GTCGTGACTGGCGTAGCTGGTAG 
                 496 
                 TAGGGAAGAGAAGGACATATGATGTCGTGACTGGCGTAG 
               
               
                   
                 TGCTAGG 
                   
                 CTGGTAGTGCTAGGTTGACTAGTACATGACCACTTGA 
               
               
                   
               
            
           
         
       
     
                     TABLE 4                  Aptamers against tree nut antigen                             SEQ ID NO.   Unique Sequence (5′-3′)   SEQ ID NO.   Full sequence including primers (5′-3′)               497   GTCATTCCGCATCCTACACGTGG   597   TAGGGAAGAGAAGGACATATGATGTCATTCCGCATCCTA           GCACGTA       CACGTGGGCACGTATTGACTAGTACATGACCACTTGA               498   GGCATGCACGCACCTACAGTGGG   598   TAGGGAAGAGAAGGACATATGATGGCATGCACGCACCTA           TATGGA       CACGTGGGTATGGATTGACTAGTACATGACCACTTGA               499   GGCCTGCGATGGATGTGTGCGTG   599   TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT           TATTAGC       GTGCGTGTATTAGCTTGACTAGTACATGACCACTTGA               500   GCACGACTCGCACCCTATCATGA   600   TAGGGAAGAGAAGGACATATGATGCACGACTCGCACCCT           GGCCATG       ATCATGAGGCCATGTTGACTAGTACATGACCACTTGA               501   GTCATGCCATCCCTTCGAGTGTG   601   TAGGGAAGAGAAGGACATATGATGTCATGCCATCCCTTC           ACAGGTA       GAGTGTGACAGGTATTGACTAGTACATGACCACTTGA               502   GGCCTGCGATGGATGAGGTGCTG   602   TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGA           CACTAGT       GGTGCTGCACTAGTTTGACTAGTACATGACCACTTGA               503   GGACGCAGCTTGCCTACTGGTGG   603   TAGGGAAGAGAAGGACATATGATGGACGCAGCTTGCCTA           TCACGTA       CTGGTGGTCACGTATTGACTAGTACATGACCACTTGA               504   GGCCAATGCGCCCCTTCATGTTG   604   TAGGGAAGAGAAGGACATATGATGGCCAATGCGCCCCTT           TCGTGTA       CATGTTGTCGTGTATTGACTAGTACATGACCACTTGA               505   GTGCGCACGATGGATGTGGATGG   605   TAGGGAAGAGAAGGACATATGATGTGCGCACGATGGATG           CCAGTCT       TGGATGGCCAGTCTTTGACTAGTACATGACCACTTGA               506   GTCATGCGCGGACATTCAAGTTG   606   TAGGGAAGAGAAGGACATATGATGTCATGCGCGGACATT           GCGTGGA       CAAGTTGGCGTGGATTGACTAGTACATGACCACTTGA               507   CCGCACGTAGCCCTATCAGTGGT   607   TAGGGAAGAGAAGGACATATGATCCGCACGTAGCCCTAT           GCATGCA       CAGTGGTGCATGCATTGACTAGTACATGACCACTTGA               508   GGCATGCGCTGGGTAGTGATCAC   608   TAGGGAAGAGAAGGACATATGATGGCATGCGCTGGGTAG           GTACGGGT       TGATCACGTACGGTTTGACTAGTACATGACCACTTGA               509   GGCCTGCGATGGATGTGGGCTAG   609   TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGATGT           TATGGGC       GGGCTAGTATGGGCTTGACTAGTACATGACCACTTGA               510   GTGCGACCGACCCTATCAGGTGC   610   TAGGGAAGAGAAGGACATATGATGTGCGACCGACCCTAT           TCATGTA       CAGGTGCTCATGTATTGACTAGTACATGACCACTTGA               511   GTACTGCACTGCCCTACACGTGG   611   TAGGGAAGAGAAGGACATATGATGTACTGCACTGCCCTA           GAATGGA       CACGTGGGAATGGATTGACTAGTACATGACCACTTGA               512   GGCCGACCTGGATGTGAGCATGC   612   TAGGGAAGAGAAGGACATATGATGGCCGACCTGGATGTG           ATCTAGT       AGCATGCATCTAGTTTGACTAGTACATGACCACTTGA               513   CGCACCGTCGATACGTCATGCAC   613   TAGGGAAGAGAAGGACATATGATCGCACCGTCGATACGT           GCTGACA       CATGCACGCTGACATTGACTAGTACATGACCACTTGA               514   GGCATGCGATGGATGGGGGCTGG   614   TAGGGAAGAGAAGGACATATGATGGCATGCGATGGATGG           GGCAGTC       GGGCTGGGGCAGTCTTGACTAGTACATGACCACTTGA               515   GACGGTGCGTCCTAAAGTGCTCA   615   TAGGGAAGAGAAGGACATATGATGACGGTGCGTCCTAAA           GTGCGTG       GTGCTCAGTGCGTGTTGACTAGTACATGACCACTTGA               516   GTACGCATCGTCCCGTCATGTGG   616   TAGGGAAGAGAAGGACATATGATGTACGCATCGTCCCGT           TTCCGTA       CATGTGGTTCCGTATTGACTAGTACATGACCACTTGA               517   GTGCGACCTGACCTAGCAAGCGG   617   TAGGGAAGAGAAGGACATATGATGTGCGACCTGACCTAG           TAGTGTA       CAAGCGGTAGTGTATTGACTAGTACATGACCACTTGA               518   GTACGACGCGGACCTTCAAGTAG   618   TAGGGAAGAGAAGGACATATGATGTACGACGCGGACCTT           GCGTGTA       CAAGTAGGCGTGTATTGACTAGTACATGACCACTTGA               519   GGCCAAGCTGACCGTAAAGGCAG   619   TAGGGAAGAGAAGGACATATGATGGCCAAGCTGACCGTA           GCAGTGTA       AAGGCAGGCGTGTATTGACTAGTACATGACCACTTGA               520   GTACGCGACGTCCCTGAGAGTGT   620   TAGGGAAGAGAAGGACATATGATGTACGCGACGTCCCTG           GCAGGTA       AGAGTGTGCAGGTATTGACTAGTACATGACCACTTGA               521   GTCAGTCGATGGATGTGGGTTGT   621   TAGGGAAGAGAAGGACATATGATGTCAGTCGATGGATGT           GCTCGTC       GGGTTGTGCTCGTCTTGACTAGTACATGACCACTTGA               522   CGCACCGTCAAGCGGGAAGGCAC   622   TAGGGAAGAGAAGGACATATGATCGCACCGTCAAGCGGG           TTTGGTG       AAGGCACTTTGGTGTTGACTAGTACATGACCACTTGA               523   GGACGCACGCAGACCTTCAAGTC   623   TAGGGAAGAGAAGGACATATGATGGACGCACGCAGACCT           GGCCATG       TCAAGTCGGCCATGTTGACTAGTACATGACCACTTGA               524   CCGTAGCGACATCAAGCGGTGGT   624   TAGGGAAGAGAAGGACATATGATCCGTAGCGACATCAAG           GTGCGTG       CGGTGGTGTGCGTGTTGACTAGTACATGACCACTTGA               525   GTGCGCGGCTTGCCTTCACGTGA   625   TAGGGAAGAGAAGGACATATGATGTGCGCGGCTTGCCTT           TCGTGTA       CACGTGATCGTGTATTGACTAGTACATGACCACTTGA               526   GGCCTGATCGAACCTAGAGAGTG   626   TAGGGAAGAGAAGGACATATGATGGCCTGATCGAACCTA           GCGTGGA       GAGAGTGGCGTGGATTGACTAGTACATGACCACTTGA               527   GGACGCATCGCCCCTTCGAGTGG   627   TAGGGAAGAGAAGGACATATGATGGACGCATCGCCCCTT           ACAGGTA       CGAGTGGACAGGTATTGACTAGTACATGACCACTTGA               528   GTACGCACGATGAGCGCCAAGTG   628   TAGGGAAGAGAAGGACATATGATGTACGCACGATGAGCG           ACATGGA       CCAAGTGACATGGATTGACTAGTACATGACCACTTGA               529   GGCATGCGATGGATGTGGTGTAC   629   TAGGGAAGAGAAGGACATATGATGGCATGCGATGGATGT           CCAGTCC       GGTGTACCCAGTCCTTGACTAGTACATGACCACTTGA               530   GGACGGAACGTGAGGGCAAGTAC   630   TAGGGAAGAGAAGGACATATGATGGACGGAACGTGAGGG           GTGCTCG       CAAGTACGTGCTCGTTGACTAGTACATGACCACTTGA               531   CCATCGCGTCACATCATGTGTGT   631   TAGGGAAGAGAAGGACATATGATCCATCGCGTCACTATC           CACTGC       ATGTGTGTCACGTATTGACTAGTACATGACCACTTGA               532   GTCGTGACTGGCTAGCTGGACAT   632   TAGGGAAGAGAAGGACATATGATGTCGTGACTGGCTAGC           GCACTGC       TGGACATGCACTGCTTGACTAGTACATGACCACTTGA               533   GGACTGCGCGTGACCTATCAATG   633   TAGGGAAGAGAAGGACATATGATGGACTGCGCGTGACCT           GCATGTA       ATCAATGGCATGTATTGACTAGTACATGACCACTTGA               534   GACCTGACTGTGCCTATCGAGTG   634   TAGGGAAGAGAAGGACATATGATGACCTGACTGTGCCTA           CGTGATG       TCGAGTGCGTGATGTTGACTAGTACATGACCACTTGA               535   AATGCGGCATGAACGGACCTACA   635   TAGGGAAGAGAAGGACATATGATAATGCGGCATGAACGG           CGTGGGC       ACCTACACGTGGGCTTGACTAGTACATGACCACTTGA               536   GCATTGGACGATCGTGCCCTACA   636   TAGGGAAGAGAAGGACATATGATGCATTGGACGATCGTG           CGTGGGC       CCCTACACGTGGGCTTGACTAGTACATGACCACTTGA               537   GGCCACACGCTGCCCTAAAGTGT   637   TAGGGAAGAGAAGGACATATGATGGCCACACGCTGCCCT           TCGTGTA       AAAGTGTTCGTGTATTGACTAGTACATGACCACTTGA               538   GTACGGAACGGAACAACAAGGGC   638   TAGGGAAGAGAAGGACATATGATGTACGGAACGGAACAA           AGGCATG       CAAGGGCAGGCATGTTGACTAGTACATGACCACTTGA               539   GGCCAGATCGACATAGCGAGTGA   639   TAGGGAAGAGAAGGACATATGATGGCCAGATCGACATAG           GAGTGTA       CGAGTGAGAGTGTATTGACTAGTACATGACCACTTGA               540   GTCATGCGCGTACCATCGAGGGG   640   TAGGGAAGAGAAGGACATATGATGTCATGCGCGTACCAT           GCGTGGA       CGAGGGGGCGTGGATTGACTAGTACATGACCACTTGA               541   GCACGCCGATGCCCTCATGTGGC   641   TAGGGAAGAGAAGGACATATGATGCACGCCGATGCCCTC           CGTGGA       ATGTGGCCGTGGATTGACTAGTACATGACCACTTGA               542   GCACTGAGCGTACGTATCAGCGG   642   TAGGGAAGAGAAGGACATATGATGCACTGAGCGTACGTA           GCACGTA       TCAGCGGGCACGTATTGACTAGTACATGACCACTTGA               543   GTGCTGAGCTGGGAGTGGTGGTG   643   TAGGGAAGAGAAGGACATATGATGTGCTGAGCTGGGAGT           CTTTGGC       GGTGGTGCTTTGGCTTGACTAGTACATGACCACTTGA               544   GTGCTGCGATGGATTGGGAGTGT   644   TAGGGAAGAGAAGGACATATGATGTGCTGCGATGGATTG           CTTTGGC       GGAGTGTCTTTGGCTTGACTAGTACATGACCACTTGA               545   GTACGCGGCTGGATACAAGTACG   645   TAGGGAAGAGAAGGACATATGATGTACGCGGCTGGATAC           GCATGGA       AAGTACGGCATGGATTGACTAGTACATGACCACTTGA               546   GGACGCGATGGATGTGGACGGTG   646   TAGGGAAGAGAAGGACATATGATGGACGCGATGGATGTG           TGGTAGT       GACGGTGTGGTAGTTTGACTAGTACATGACCACTTGA               547   GTCATGCACGAACACTATCATGG   647   TAGGGAAGAGAAGGACATATGATGTCATGCACGAACACT           CGGCATG       ATCATGGCGGCATGTTGACTAGTACATGACCACTTGA               548   GTCATGCTCGCACTATCAGGTGT   648   TAGGGAAGAGAAGGACATATGATGTCATGCTCGCACTAT           GCATGGA       CAGGTGTGCATGGATTGACTAGTACATGACCACTTGA               549   GGCCTGACTGGGTGTGGTTAGGA   649   TAGGGAAGAGAAGGACATATGATGGCCTGACTGGGTGTG           ACGCGTC       GTTAGGAACGCGTCTTGACTAGTACATGACCACTTGA               550   GTGCTCGCAAGACCTACACGTGG   650   TAGGGAAGAGAAGGACATATGATGTGCTCGCAAGACCTA           ACGTGGA       CACGTGGACGTGGATTGACTAGTACATGACCACTTGA               551   GGCACAAACGGACCGACAAGTGC   651   TAGGGAAGAGAAGGACATATGATGGCACAAACGGACCGA           GCATGGA       CAAGTGCGCATGGATTGACTAGTACATGACCACTTGA               552   TCGATCGATCCGTCAAGCAGGCA   652   TAGGGAAGAGAAGGACATATGATTCGATCGATCCGTCAA           CGTGTCA       GCAGGCACGTGTCATTGACTAGTACATGACCACTTGA               553   GGCATTGCGCGCCTAGCAAGTTG   653   TAGGGAAGAGAAGGACATATGATGGCATTGCGCGCCTAG           ACGTGTA       CAAGTTGACGTGTATTGACTAGTACATGACCACTTGA               554   GGACGCGTCGACTTCAAGTGTGC   654   TAGGGAAGAGAAGGACATATGATGGACGCGTCGACCTTC           CGTGGA       AAGTGTGCCGTGGATTGACTAGTACATGACCACTTGA               555   CCGCATCGGACCGATCAAGGCAG   655   TAGGGAAGAGAAGGACATATGATCCGCATCGGACCGATC           GCTTGGA       AAGGCAGGCTTGGATTGACTAGTACATGACCACTTGA               556   TGGCCAAGCGACCTAGCAAGTGT   656   TAGGGAAGAGAAGGACATATGATTGGCCAAGCGACCTAG           GCTCATG       CAAGTGTGCTCATGTTGACTAGTACATGACCACTTGA               557   GGCATGCGATGAACGAGGCATGA   657   TAGGGAAGAGAAGGACATATGATGGCATGCGATGAACGA           TGCGTCA       GGCATGATGCGTCATTGACTAGTACATGACCACTTGA               558   GTACGACGCGAGCTAGCAAGGAG   658   TAGGGAAGAGAAGGACATATGATGTACGACGCGAGCTAG           GCGTGTA       CAAGGAGGCGTGTATTGACTAGTACATGACCACTTGA               559   GGCATGCACGCACCTACACGTGG   659   TAGGGAAGAGAAGGACATATGATGGCATGCACGCACCTA           GCACGTA       CACGTGGGCACGTATTGACTAGTACATGACCACTTGA               560   CCATATGGCAGTGCGATGGCTTC   660   TAGGGAAGAGAAGGACATATGATCCATATGGCAGTGCGA           GCTGGTC       TGGCTTCGCTGGTCTTGACTAGTACATGACCACTTGA               561   GTCATGCGCTGGGTGTGGCCTGT   661   TAGGGAAGAGAAGGACATATGATGTCATGCGCTGGGTGT           TGTAGGC       GGCCTGTTGTAGGCTTGACTAGTACATGACCACTTGA               562   GTGCTGCCTGACCCACGTGGACT   662   TAGGGAAGAGAAGGACATATGATGTGCTGCCTGACCCAC           TGCACTA       GTGGACTTGCACTATTGACTAGTACATGACCACTTGA               563   GGACTGACGAGCCGTTCATGTGG   663   TAGGGAAGAGAAGGACATATGATGGACTGACGAGCCGTT           TTGTGGA       CATGTGGTTGTGGATTGACTAGTACATGACCACTTGA               564   CGCAACGGTGACGAGCAGTGAGT   664   TAGGGAAGAGAAGGACATATGATCGCAACGGTGACGAGC           GCATGTA       AGTGAGTGCATGTATTGACTAGTACATGACCACTTGA               565   GGACGGATCGAACCTACAAGTTG   665   TAGGGAAGAGAAGGACATATGATGGACGGATCGAACCTA           TCGTGGA       CAAGTTGTCGTGGATTGACTAGTACATGACCACTTGA               566   GTCGTGAGCTGGAAGGAGAGTGG   666   TAGGGAAGAGAAGGACATATGATGTCGTGAGCTGGAAGG           GTACGTA       AGAGTGGGTACGTATTGACTAGTACATGACCACTTGA               567   GTCATTGTCGGATGTGAGCATGT   667   TAGGGAAGAGAAGGACATATGATGTCATTGTCGGATGTG           TTCTCGGC       AGCATGTTCTCGGCTTGACTAGTACATGACCACTTGA               568   GCGTGTCAGCAATACGTCCTCAT   668   TAGGGAAGAGAAGGACATATGATGCGTGTCAGCAATACG           CTGCCCG       TCCTCATCTGCCCGTTGACTAGTACATGACCACTTGA               569   GTCACGGAACGAAGCCTATCAAG   669   TAGGGAAGAGAAGGACATATGATGTCACGGAACGAAGCC           TGCGACA       TATCAAGTGCGACATTGACTAGTACATGACCACTTGA               570   GCGACAGCGACATACGATCTGCT   670   TAGGGAAGAGAAGGACATATGATGCGACAGCGACATACG           CTGCGTC       ATCTGCTCTGCGTCTTGACTAGTACATGACCACTTGA               571   GTTCGCGCGACACCTATCAATGT   671   TAGGGAAGAGAAGGACATATGATGTTCGCGCGACACCTA           GGACGTG       TCAATGTGGACGTGTTGACTAGTACATGACCACTTGA               572   GACACGCCGATGAGCCTAGCCTG   672   TAGGGAAGAGAAGGACATATGATGACACGCCGATGAGCC           TACGACG       TAGCCTGTACGACGTTGACTAGTACATGACCACTTGA               573   GGCCGAACTGGATGGGGGCATTA   673   TAGGGAAGAGAAGGACATATGATGGCCGAACTGGATGGG           CTGCGGC       GGCATTACTGCGGCTTGACTAGTACATGACCACTTGA               574   GTACGCCTCGGAGCTAGCAGGTG   674   TAGGGAAGAGAAGGACATATGATGTACGCCTCGGAGCTA           GTGTGGA       GCAGGTGGTGTGGATTGACTAGTACATGACCACTTGA               575   GGCCAAGCTGGATGTGTTCAGGT   675   TAGGGAAGAGAAGGACATATGATGGCCAAGCTGGATGTG           CACGACG       TTCAGGTCACGACGTTGACTAGTACATGACCACTTGA               576   GTCATGCTCTGGGTGTGCGAATG   676   TAGGGAAGAGAAGGACATATGATGTCATGCTCTGGGTGT           TGGTAGG       GCGAATGTGGTAGGTTGACTAGTACATGACCACTTGA               577   GTCACTGTGCCTGACCGTCAAGT   677   TAGGGAAGAGAAGGACATATGATGTCACTGTGCCTGACC           TGCGGCA       GTCAAGTTGCGGCATTGACTAGTACATGACCACTTGA               578   CGACGCAATCGGACACTGGACAT   678   TAGGGAAGAGAAGGACATATGATCGACGCAATCGGACAC           GCGCAGA       TGGACATGCGCAGATTGACTAGTACATGACCACTTGA               579   GCACGTACGCCTTGCCTATCTGT   679   TAGGGAAGAGAAGGACATATGATGCACGTACGCCTTGCC           GCTCATG       TATCTGTGCTCATGTTGACTAGTACATGACCACTTGA               580   GTGCGCCGATATCCCTTCACAGT   680   TAGGGAAGAGAAGGACATATGATGTGCGCCGATATCCCT           TGGCATG       TCACAGTTGGCATGTTGACTAGTACATGACCACTTGA               581   CATGTGTCGACTCGCCCTATCAT   681   TAGGGAAGAGAAGGACATATGATCATGTGTCGACTCGCC           GCGGTCA       CTATCATGCGGTCATTGACTAGTACATGACCACTTGA               582   GGACGCACATCCCGTTCATGTGT   682   TAGGGAAGAGAAGGACATATGATGGACGCACATCCCGTT           GCATGTA       CATGTGTGCATGTATTGACTAGTACATGACCACTTGA               583   GGACTCGCGTCACTATCACGGGG   683   TAGGGAAGAGAAGGACATATGATGGACTCGCGTCACTAT           GCAGGTA       CACGGGGGCAGGTATTGACTAGTACATGACCACTTGA               584   GCTTCGATGGATGCTGGGCAGGC   684   TAGGGAAGAGAAGGACATATGATGCTTCGATGGATGCTG           ACGCAGT       GGCAGGCACGCAGTTTGACTAGTACATGACCACTTGA               585   GACATTCGCTGGATGTGGGGATG   685   TAGGGAAGAGAAGGACATATGATGACATTCGCTGGATGT           CACTGTC       GGGGATGCACTGTCTTGACTAGTACATGACCACTTGA               586   TGGCCGATCGACCCTATCAAGTG   686   TAGGGAAGAGAAGGACATATGATTGGCCGATCGACCCTA           CAGCATG       TCAAGTGCAGCATGTTGACTAGTACATGACCACTTGA               587   GTGCGACCGACCCTATCAAGTAC   687   TAGGGAAGAGAAGGACATATGATGTGCGACCGACCCTAT           GTCA       CAAGTACGTCATTGACTAGTACATGACCACTTGA               588   GTGCTGAACGTACCGATTCAAGT   688   TAGGGAAGAGAAGGACATATGATGTGCTGAACGTACCGA           GTGCGTG       TTCAAGTGTGCGTGTTGACTAGTACATGACCACTTGA               589   GGCCTGCGATGGAATGTGCGAAT   689   TAGGGAAGAGAAGGACATATGATGGCCTGCGATGGAATG           GTACGCA       TGCGAATGTACGCATTGACTAGTACATGACCACTTGA               590   CTCATGCGGACCGAACTGGATGT   690   TAGGGAAGAGAAGGACATATGATCTCATGCGGACCGAAC           GTGCATG       TGGATGTGTGCATGTTGACTAGTACATGACCACTTGA               591   GTTCTGACTGGTGTGGTGCTGCA   691   TAGGGAAGAGAAGGACATATGATGTTCTGACTGGGTGTG           CTGTCA       GTGCTGCACTGTCATTGACTAGTACATGACCACTTGA               592   GTCGTGCGTGCCCTATCAAGTCG   692   TAGGGAAGAGAAGGACATATGATGTCGTGCGTGCCCTAT           GTCTGTA       CAAGTCGGTCTGTATTGACTAGTACATGACCACTTGA               593   GGCCGAATGACCGTCTCATGTGA   693               GCATGGA                       594   GGTCCGAACGCACCTCATGTGTG   694               TCGTGTA                       595   GGTCTGCGCGTACCGTCAAGTGC   695               GAATGGA                       596   GTCGTGAGCGGGTGTGGGACTGG   696               ACGCAGT                    
4. SPN-Complement Complexes
 
     In some embodiments, SPN-complement complexes are provided. A nucleic acid sequence that is complementary to a portion of the sequence of a signaling polynucleotide (SPN) (e.g., either the 5′end or 3′end sequence of the SPN) is hybridized with the corresponding SPN sequence, forming a SPN-complement complex. In some aspects, the complementary sequence may contain about 5 to 20 nucleotide residues, or about 5 to 10 nucleotide residues, or about 10 to 15 nucleotide residues, or about 10-20 nucleotide residues. In particular, it may contain 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleotide residues. In one embodiment, the complementary sequence contains 5 nucleotide residues. In another embodiment, the complementary sequence contains 10 nucleotide residues. In some aspects, the complementary sequence may be at least 100% complementary to the SPN sequence, or at least 99% complementary to the SPN sequence, or at least 95% complementary to the SPN sequence, or at least 90% complementary to the SPN sequence, or least 80% complementary to the SPN sequence. In another embodiment, the complementary sequence may have additional polyA nucleotides. The short complementary sequence can easily detach from the corresponding SPN, in particular when a target allergen protein competes and binds to the SPN, enabling a highly sensitive detection assay. 
     In some aspects, the complementary sequence is labeled with a detection signal moiety e.g., a fluorophore, at either the 5′end or 3′end of the sequence. As non-limiting examples, the fluorophore may be selected from Alex Fluor® @ fluorophores (such as Alex 514, Alex 532, Alex 546, Alex 555, Alex 568, Alex 594, Alex 610, Alex 633, Alex 635, Alex 647, Alex 660, Alex 680, Alexa 700, Alex 750, Alex 800, Alex 610-R-phycoerythrin (R-PE), Alex 647-R-phycoerythrin (R-PE), Alex 680-R-phycoerythrin (R-PE), and Alex 680-Allophycocyanin (APC)), Allophycocyanin (APC) and its derivatives, Cy fluorophores (e.g., Cy3.5, Cy3-FITC, CY5, CY 5.5, CY7, CY7-APC, CY5.5-APC), Qdots, TRITC, R-PE, Tamara, Rhodamine Red-X, Rox, TruRed, SYPRO red, BODIPY TR, Propidium iodide and Texas red. In some examples, the fluorophore is Alex 647, Cy5, Cy3-FITC or Texas red. 
     Detection Agents 
     Aptamers, signaling polynucleotides (SPNs) and SPN-complement complexes can be used as detection agents in a variety of allergen detection assays, biosensors, detection systems and devices as disclosed in the prior art, either as free agents or conjugates to other support substances. For example, aptamers, SPNs and SPN-complement complexes of the present invention may be used as surface bound affinity molecules that bind the surfaces of solid substrates. The solid surface may be a three-dimensional surface such as micro-spheres (e.g., magnetic beads/particles), or a two-dimensional surface such as the glass or silicon surface. 
     In one embodiment, aptamers, SPNs and SPN-complement complexes of the present invention are immobilized on the surface of magnetic particles to form functionalized magnetic particles which can capture a target analyte (e.g., allergen) in a fluid sample; the particles will be suitable for magnetic manipulations in a detection assay and method. 
     In some aspects, aptamers, SPNs and SPN-complement complexes of the present invention may be covalently immobilized on the surface of magnetic particles. In some aspects, SPNs may be covalently immobilized on the surface of magnetic particles through an amine (—NH2) group, or a thiol group (—SH) at one end the SPN sequence. In other aspects, complementary sequence of SPNs may be covalently immobilized on the surface of magnetic particles, from which the SPNs are attached to the particles. Concentrations of SPNs, complementary sequences and magnetic particles are optimized for the most effective binding to each other. In some aspects, SPNs and their complementary sequences may be at a ratio of 1:5, or at a ratio of 1:4, or at a ratio of 1:3, or at a ratio of 1:2. 
     In another embodiment, aptamers, SPNs and SPN-complement complexes of the present invention may be attached to a two-dimensional solid surface; said two dimensional solid surface may be a glass surface or the surface of a silicon chip. Such surfaces printed/coated with aptamers, SPNs, complements and/or SPN-complement complexes of the present invention may be used as biosensing platforms for a variety of assays and applications. 
     In some embodiments, detection agents of the present invention may be labeled with a fluorescent marker which generates detectable fluorescent signals. Either the SPN or its complementary sequence may be labeled with a fluorophore. In some aspects, the fluorophore is added to one end of the complement sequence, either the 5′end or 3′end, as illustrated in  FIGS. 4A, 4B and 4C , and  FIG. 5A . In other aspects, One end of the SPN may be labeled with a fluorophore, as shown in  FIG. 5B .  FIG. 6A  and  FIG. 6B . As non-limiting examples, the fluorophore may be selected from, Alex Fluor®, fluorophores (such as Alex 514, Alex 532, Alex 546, Alex 555, Alex 568, Alex 594, Alex 610, Alex 633, Alex 635, Alex 647, Alex 660, Alex 680, Alexa 700, Alex 750, Alex 800, Alex 610-R-phycoerythrin (R-PE), Alex 647-R-phycoerythrin (R-PE), Alex 680-R-phycoerythrin (R-PE), and Alex 680-Allophycocyanin (APC)), Allophycocyanin (APC) and its derivatives, Cy fluorophores (e.g., Cy3.5, Cy3-FITC, CY5, CY 5.5, CY7, CY7-APC, CY5.5-APC), Qdots, TRITC, R-PE, Tamara, Rhodamine Red-X, Rox, TruRed, SYPRO red, BODIPY TR, Propidium iodide and Texas red. In some examples, the fluorophore is Alex 647, Cy5, CY3-FITC or Texas red. 
     1. Magnetic Particles/Beads 
     Magnetic particles have several advantages that make them attractive substrates for use as signal transducers, including their biological inertness, physical stability, and the absence of competing magnetic signals in biological materials (Gijs et al.,  Chem Rev.  2010; Vol 110(3), 1518-1563). 
     Magnetic particles may be any particle materials that can be separated by magnetic forces. Magnetic particles for bioresearch may consist of one or more magnetic cores with a coating matrix of polymers, silica or hydroxylapatite with terminal functionalized groups. The magnetic core generally consists either of magnetite (Fe 3 O 4 ) or maghemite (γ-Fe 2 O 3 ) with superparamagnetic or ferromagnetic properties. For example, magnetic cores can be made with magnetic ferrites, such as cobalt ferrite or manganese ferrite. Such magnetic micro- or nanospheres can be separated easily and quickly by magnetic forces and can be used together with bioaffine ligands, e.g. antibodies or aptamers with a high affinity to the target. 
     In one example, magnetic particles or beads may be synthesized by dispersing ferrite crystals in a suspension of styrene, divinylbenzene monomers and polymerizing the cocktails into microparticles. In another example, magnetic particles/beads may be synthesized by coating one or more layers of magnetite onto a polystyrene core. Multiple layers of magnetite may increase the speed by which the particle/bead responds to a magnetic field. The synthesized microparticles are encapsulated with inert polymers such as polystyrene to prevent the iron from interfering with any subsequent biochemical reactions. Polystyrene magnetic beads can be used for with both carboxylic acid and amine surface chemistries. 
     Additional polymers that may be used to prepare magnetic particles/beads include, but are not limited to, alginate, dextran, polyacrylamide, polycaprolactone, polyethylenimine (PEI), polyisopropene, poly(2-cinnamoylethyl methacrylate), poly(acetoacetoxyethyl methacrylate), poly(dimethylsiloxane), poly(ethylene glycol)-poly(aspartic acid) (PEG-PAsp), poly(ethylene oxide), poly(glutamic acid), poly(glycidyl methacrylate), poly(lactide), poly(lactide-co-glycolide), poly(1-lactic acid), poly(l-lactide-co-glycolide), poly(methyl methacrylate-divinylbenzene), poly(N-isopropyl acrylamide) (PNIPA), poly(N-vinylcaprolactam), poly(styrene-acetoacetoxyethylmethacrylate) (PSAAEM), poly(styrene-butyl acrylate-methacrylic acid), poly(styrene-co-acrylamide), poly(styrene-co-acrylic acid), poly(styrene-co-butyl acrylate), poly(styrene-divinylbenzene-glycidyl methacrylate), poly(styrene-methacrylic acidacrylamide), poly(tert-butyl acrylate), poly(vinyl alcohol) (PVA), and any combinations thereof. 
     Magnetic particles may also be coated with streptavidin, maleimide, amino groups or carboxyl groups to optimize aptamer affinity. DNA specificity and non-specific adsorption of DNAs on their surfaces. 
     In addition to chemical modifications on the surface, magnetic particles may be in a size with maximal efficiency and affinity to the conjugated nucleic acids. As used herein, the particle size (or particle diameter) is given as a hydrodynamic diameter, which includes the core diameter and two times the diameter of the cover matrix. The size of the particle determines the surface area available for the attachment of functional groups. Increasing the size reduces the surface-to-volume ratio therefore resulting in a decrease of the available surface area per volume for modification, but speeding up its response in a magnetic field which makes it easier to be manipulated. In some examples, magnetic particles may be in a wide range of average particle sizes, from 100 nm to 5.0 μm in the diameter, having optimized parameters such as sedimentation rate, available binding sites, and magnetic volume. In some aspects, the average magnetic particle size may be from 100 nm to 800 nm, or from 200 nm to 500 nm, or from 0.1 μm to 3.0 μm, the size may be 100 nm, 125 nm, 150 nm, 200 nm, 250 nm, 300 nm, 350 nm, 400 nm, 450 nm, 500 nm, 550 nm, 600 nm, 700 nm, 800 nm, 900 nm, 0.2 μm, 0.3 μm, 0.4 μm, 0.5 μm, 0.6 μm, 0.7 μm, 0.8 μm, 0.9 μm, 1.0 μm, 1.5 μm, 2.0 μm, 2.5 μm, 3.0 μm, 3.5 μm, 4.0 μm, 4.5 μm, or 5.0 μm. 
     In some cases, magnetic particles may have an irregular or rough surface. As curvature can introduce additional surface area, irregular or rough surface increases the overall surface area available for the attachment of nucleic acid molecules. 
     As non-limiting examples, magnetic particles may be fluidMAG particles (which is hydrophilic). SiMAG particles (which are magnetic silica beads with superparamagnetic or ferromagnetic properties and possess either a highly porous or a non-porous silica surface), mHPA-particles (which are non-spherical with hydroxylapatite coated ferromagnetic particles with a diameter of 2 μm, consisting of calcium phosphate), ZeoliteMAG (which are magnetic zeolite particles, which consist of a superparamagnetic iron oxide core and a high-porous aluminosilicate matrix), beadMAG-particles (which are magnetic particles with a diameter of 1 μm, covered with a hydrophilic matrix of crosslinked starch with terminal cation-exchange phosphate groups), and magTosyl-magnetic beads or other appropriately derived magnetic beads for nucleic acid conjugations. In some embodiments, polystyrene magnetic particles may be used. The magnetic particles may also be sepharose magnetic microbeads and agarose magnetic microbeads. 
     In some embodiments, magnetic particles, in the absence of a magnetic field, may exhibit no net magnetization, but within a magnetic field, the magnetic moments of the bead align with the field, making the beads magnetic. 
     2. Other Solid Substrates 
     Aptamers, SPNs and/or SPN-complement complexes may be attached to any two-dimensional solid surfaces such as microtiter plates, silicon chips and printed glass surfaces (e.g. epoxy saline-derived glass surfaces). Other examples of suitable solid substrates (also called solid supports) may include, but are not limited to, those made of silica or silica-based materials, functionalized glass, modified silicon, inorganic glasses, plastics, resins, polysaccharides, carbon, metals, nylon, natural fibers such as silk, wool and cotton, and polymers. Solid substrates may have any useful form including thin films or membranes, beads, microwell plates, dishes, slides, fibers, woven fibers, shaped polymers, particles, chips, wafers and microparticles. Solid substrates may be porous or non-porous. 
     In some embodiments, the two-dimensional surface may be a surface of a glass or silicon slide. Glass is a readily available and inexpensive support medium that has low intrinsic fluorescence. The surface of the glass or silicon slide may be modified or unmodified, although most attachment protocols involve chemically modifying the glass surface to facilitate attachment of the oligonucleotides. Glass has a relatively homogeneous chemical surface whose properties have been well studied and is amenable to chemical modification using very versatile and well developed silanization chemistry. In certain embodiments, the surface of the glass or silicon slide is unmodified. For example, silianized oligonucleotides can be covalently linked to an unmodified glass surface (Kumar et al.,  Nucleic Acids Res.  2000 Jul. 15; 28(14): e71). 
     In certain embodiments, the surface of glass or silicon is chemically modified. Glass surface can be treated with an amino silane to have a uniform layer of primary amines or epoxides. In one example, oligonucleotides modified with an NH2 group can be immobilized onto epoxy silane-derivatized or isothiocyanate coated glass slides. In another example, succinylated oligonucleotides can be coupled to aminophenyl- or aminopropyl-derivitized glass slides by peptide bonds. In yet another example, disulfide-modified oligonucleotides can be immobilized onto a mercaptosilanized glass support by a thiol/disulfide exchange reactions or through chemical cross linkers. 
     In some embodiments, graphene oxide (GO) may be used as signal transducers in replacement of magnetic particles. Graphene oxide (GO) is a single-atom-thick two-dimensional carbon nanomaterial widely used in biosensor applications due to its unique optical electronic, thermal, and long-lasting biocompatibility properties (also known as graphene oxide nanosheet and graphene nanosheet). Most importantly. GO has superior fluorescence quenching capacity and unique adsorption characteristics for biomolecules. The quenching capacity is due to self-assembly of graphene oxide through specific π-π interactions. 
     GO is graphite oxidized to intersperse the carbon layers with oxygen molecules, and then reduced to separate the carbon layers completely into individual or few layer graphene. GO can be synthesized by the oxidative treatment of graphite by one of the principle methods developed by Brodie, Hummers or Staudenmeir in the art. A number of modified methods are also available (Paulchamy, et al.,  J Nanomed Nanotechnol,  2015, 6:1. doi: 10.4172/2157-7439.1000253; Jasim, et al., 2 D Mater.  2016, 3, 014006. doi: 10.1088/2053-1583/3/1/014006; and Shahriary and Athawale,  Int. J. Renew. Energy Environ. Eng,  2014, Vol. 02 (01): 58-63; the contents of each of which are incorporated herein by reference in their entirety.) 
     In some embodiments, SPNs and/or SPN-complement complexes may be attached onto the GO surface through physical adsorption. In this aspect, SPNs or SPN-complement complexes may be labeled with Qdots. When Qdots-aptamer conjugates are attached onto GO, the fluorescence signal is quenched due to non-radioactive electronic excitation energy transfer between the fluorophore and GO. In the presence of a target molecule, the interaction between the target molecule and the aptamer is stronger than that between the aptamer and GO, resulting in the release of the aptamer and therefore recovery of the fluorescence signal. 
     3. Conjugation Chemistry and Methods 
     Aptamers, SPNs and SPN complementary sequences can be conjugated to magnetic particles by any method known in the art which are used to conjugate nucleic acid molecules to solid surfaces. The methods may be irreversible immobilization methods or reversible immobilization methods. As non-limiting examples, methods may include biotin-streptavidin system, and EDC mediated carboxyl-to-amine crosslinking (e.g., covalent binding of amino-modified aptamer (SPNs) to carboxyl-functionalized magnetic particles), Aldehyde-activated sugars; and cross linkers used to modify nucleic acids and solid surfaces for conjugations (Scientific, Thermo (2012),  Crosslinking Technical Handbook , Thermo Fisher Scientific Inc., Waltham, USA). 
     In some embodiments, the short complementary sequences may be bound to a solid surface. In other embodiments, the SPN may be bound to a solid surface. 
     In some embodiments, nucleic acid molecules (e.g. aptamers, SPNs, and SPN complement) of the present invention are conjugated to the surface of magnetic particles at one end, e.g. the 5′ end or 3′end (e.g., as shown in  FIGS. 4A, 4B, 4C  and  FIG. 5B ). Nucleic acids can be covalently attached to magnetic particles/beads by methods based on the formation of covalent bonds. Carboxyl and amino groups are the most common reactive groups for attaching ligands to surfaces. Examples of the reactive groups that can be incorporated on the micro-bead surface and/or the termini of ligands for covalent coupling may include but are not limited to, carboxylic acid (—COOH). Primary aliphatic amine (—RNH 2 ), Aromatic amine (—ArNH 2 ), Chloromethyl (vinyl benzyl chloride) (—ArCH 2 CL), Amide (—CONH 2 ), Hydrazide (—CONHNH 2 ), Aldehyde (—CHO), Hydroxyl (—OH), Thiol (—SH) and Epoxy (—COC—). 
     In some aspects, an amino group may be attached to the 5′ or 3′ end of the nucleic acid sequence. Various amino modifiers such as β-cyanoethyl phosphoramidites can be added to the 5′ end of a nucleic acid molecule. 5′ amino modifiers can be simple amino groups with a six or twelve carbon spacers, a Uni-Link amino modifier, or amino modified thymidine or cytosine. Amino modifiers that can be added to the 3′ end of a nucleic acid molecule (e.g., SPN), may be CPGs. In one example, a primary amine (—NH2) modifier may be placed to the 5′end, or 3′end of a nucleic acid molecule (e.g., SPN), or internally using an amino-C or amino-T modified base. The amino-modified nucleic acid molecules may be attached to magnetic particles using an acylating reagent, including but not limiting to Carbodiimide (EDC), Isothiocyanate, Sulfonyl chloride, and Succinimidyl esters (NHS-ester). In one aspect, amine-modified nucleic acids can then be reacted with carboxylate-modified microparticles with carbodiimide mediated acylation in a one-step coupling, which is fast and inexpensive. In addition, Acylation of a carboxyl group generates a stable carbonyl amide. One example of carbodiimide may be water soluble EDAC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride). Alternatively, amine-modified nucleic acids may be attached to magnetic particles with Isothiocyanates, which form thioureas linkage upon reaction with amines. The thioureas linkage is relatively stable. 
     In other aspects, a thiol group (e.g., thiol meliamide) is attached to the 5′ or 3′ end of a nucleic acid molecule (e.g., a SPN). The thiol (—SH) modifier enables covalent attachment of a nucleic acid molecule to a variety of substrates including magnetic particles, via disulfide bond (—S—S—) or maleimide linkages. The incorporation of a thiol group at the 5′ end of a nucleic acid molecule may be achieved with S-trityl-6-mercaptohexyl derivatives. The 3′-thiol modifier C3 S—S CPG may be used to introduce a thiol group to the 3′-end of a nucleic acid molecule. 
     In one particular example, a short polyA sequence (e.g., 5 nt) may be added at the end of the SPN or the complementary sequence. The polyA tail then is modified with a thiol group. The reaction between thiol-DNA and carboxylated magnetic beads is mediated by maleimide which is attached to Poly(ethylene glycol) (PEG) polymer coated solid substrates. It is believed that the distance created by the addition of polyA residues and PEG, increases the binding between the bead and DNA and the stability of DNA on the surface of magnetic particles. At the same time, PEG linkers may reduce non-specific binding. 
     In some examples, cross linkers may be used to attach thiol, or amine-modified nucleic acids to the surface of magnetic beads. As used herein, the term “cross-linker” means a functional chemical group that covalently binds two distinct chemical groups and generates a physical space between the two entities which provides greater accessibility and or freedom to each of the linked biomolecules. A number of cross-linkers may be used to develop covalent attachment of thiol or amine modified nucleic acids to solid surfaces, such as succinimidyl 4-[maleimidophenyl]butyrate (SMPB). 
     Magnetic particles may also be coated with streptavidin, maleimide, other amino groups or carboxyl groups to optimize aptamer affinity, DNA specificity and non-specific adsorption of DNAs on their surfaces. 
     In one example, the coupling of nucleic acids and surfaces may be through the binding of biotin-labeled nucleic acids to streptavidin-coated beads. The biotin-streptavidin linkage can tether nucleic acid molecules to the solid surface (e.g. magnetic particles). In another example, nucleic acid molecules and/or solid substrates may be coupled by ethanolamine and heterobifunctional poly (ethylene glycol) (PEG) linkers, which allow the covalent binding of nucleic acid molecules to the solid substrates (e.g. magnetic particles and glass slides). Accordingly, magnetic beads (or other solid substrates such as glass slides) are coated with PEG polymers. PEG polymers coated magnetic particles are further modified to add an active agent such as a carboxyl group, an amine group, a maleimide and a neutravidin. The PEG linkers will change the surface force, nucleic acid stability and non-specific binding. A detailed protocol is described by Janissen et al ( Nucleic acid Research,  2014, 42(18): e137; the contents of which are incorporated herein by reference in its entirety). 
     In addition to chemical modifications on the surface, magnetic beads may be in a size with maximal efficiency and affinity to the linked nucleic acids. Magnetic particles or beads may be synthesized by dispersing ferrite crystals in a suspension of styrene, divinylbenzene monomers and polymerizing the cocktails into microparticles. (Polystyrene magnetic beads can be used for with both carboxylic acid and amine surface chemistries.) 
     In some aspects, acid treated magnetic particles containing hydroxyl (OH) groups on the surface can be used to conjugate ligands including aptamers as disclosed in U.S. Patent application publication No.: US2014/0206822, the contents of which are incorporated herein by reference in its entirety. 
     In further another example, molecular spacers may be used to mediate the coupling between aptamers and magnetic particles. The method can avoid interaction between the solid surface and the aptamer conformation. 
     Aptamers, SPNs and SPN-complement complexes may also be conjugated to a glass surface or other two-dimensional surfaces through chemical modifications known in the art. In some aspects, the solid surface treatment may include, but is not limited to epoxy silane coated glass, isothiocyanate coated glass, aminophenyl or aminopropyl-derivatized glass, mercaptosilanized glass support, aldehyde or epoxide treatment. Amine (NH2), succinylation, disulfide and hydrazide (e.g., I-Linker™) may be used to modify oligonucleotide to facilitate the attachment of DNA to different surfaces. 
     A two-dimensional surface may be prepared by treating the glass surface with an amino silane which will result in a uniform layer of primary amine or epoxides. Parameters that affect the binding of oligonucleotides to the surface may be optimized to achieve the greatest specificity and DNA density. A high surface coverage of the oligonucleotide may generate a higher signaling due to higher hybridization between SPNs and their complementary sequences. The fluorescence background, chemical stability, complexity, amenability to chemical modification, surface area, loading capacity, and the degree of non-specific binding of detect molecule are adjusted for choosing appropriate surface support and conjugating chemistry. 
     For example, to increase the loading capacity of oligonucleotides on the planar surface structures of glasses, acrylamide gels can be applied to glasses to construct a—three-dimensional surface which will increase the surface area for DNA attachment. 
     4. In Situ Oligonucleotide Synthesis on Solid Support 
     In addition to attaching a synthesized aptamer, SPN or a SPN-complementary sequence to a solid support, nucleic acid molecules (e.g. aptamers, SPNs, and SPN complementary sequence) of the present invention can also be synthesized in situ on a solid support such as on magnetic particles, glass slides, wafers, microwell plates and silicon chips. Compared to the conventional method which requires the oligonucleotides to be pre-synthesized with specific end modifications to fit the surface attachment chemistry, this method can streamline the process of synthetic preparation by eliminating these steps. 
     The nucleic acid molecule can be synthesized by (a) covalently attaching one or more phosphoramidite linkers to the functional groups on the solid substrate to produce a derivatized support. (b) reacting the derivatized support with a nucleoside phosphoramidite corresponding to the first nucleotide of desired nucleotide sequence, and (c) adding nucleoside phosphoramidite stepwise until the entire oligonucleotide is assembled. Nucleoside phosphoramidites are preferred because naturally occurring nucleotides and their phosphodiester analogs are insufficiently reactive for an expeditious synthesis of oligonucleotides in high yields. Non-nucleoside phosphoramidites may be used to produce modified oligonucleotides. 
     External forces may be used to immobilize the solid substance during synthesis. For example, nucleic acid molecules may be synthesized on paramagnetic beads using the methods described in U.S. patent application Ser. No. 14/280,609 and Jensen et al.,  J Biotechnol,  2013, Sep. 20; 167(4); the contents of which are hereby incorporated by reference. The approach uses an external magnet to hold the paramagnetic beads in place during synthesis. 
     The nucleic acid synthesis can be performed in either the 5′ to 3′ direction or the 3′ to 5′ direction by suitable choice of nucleoside phosphoramidite reagents. For example, 3′-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidites can be used for synthesis in the 3′ to 5′ direction. Alternatively, 5′-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidites (Glen Research) can be used for synthesis in the 5′ to 3′ direction. 
     In certain embodiments, the substrate functional group used for attachment of the linker is a hydroxyl group or an amino group. For example, a hydroxylated substrate, such as hydroxylated polystyrene may be used for attachment of the linker. Phosphoramidite linkers are used to reduce steric hindrance associated with neighboring base-base interactions. In certain embodiments, the phosphoramidite linker is between 30 and 60 atoms in length. Exemplary phosphoramidite linkers include those disclosed in U.S. patent application Ser. No. 14/280,609; the contents of which are incorporated herein in its entirety. 
     5. Optimizing Oligonucleotide Synthesis on a Solid Support 
     In some embodiments, the short oligonucleotide sequences directly synthesized on a solid surface may be further optimized to fit the purpose of detection agents. In accordance with the present invention, a short sequence that is directly synthesized on a solid support (e.g., the surface of magnetic beads) will form a complex with the aptamer. The short oligonucleotide is complementary to one end of the aptamer sequence. The aptamer-complement complexes linked on the solid support are then used as detection agents. To fulfill this purpose, short complement sequences attached on a solid surface may meet the following features: (1) the oligonucleotides cannot be cleaved off from the surface following synthesis; (2) the oligonucleotides are being held away from the surface to allow hybridization with the complementary sequence on the aptamer to form aptamer-complement complexes; and (3) only low to moderate density of the complementary oligonucleotides are synthesized on the solid surface. 
     As described above, a standard 3′ to 5′ phosphoramidite chemical reaction may be used to for oligonucleotide synthesis. Automated synthesizers may be used to synthesize the short oligonucleotides using magnetic beads as supports. In one example, highly cross-linked polystyrene (PS) beads with proper particle sizes and good moisture exclusion properties may be used for efficient oligonucleotide synthesis. The polystyrene polymer may further bind together with magnetic particles (e.g., Fe3O4 particles) to form the solid support for direct oligonucleotide synthesis. 
     To stabilize synthesized oligonucleotide sequences on beads, non-cleavable stable linkers may be used for oligonucleotide synthesis. A non-cleavable linker is a chemical moiety immobilized on a solid support and not substantially cleaved under synthesis conditions (e.g., deprotection conditions). The linker may be covalently bound to a solid support (e.g., functionalized glass and magnetic beads). 
     Exemplary stable linkers may include, but are not limited to, amino linkers such as siloxane linkers, linkers containing alkyl groups such as —(OCH 2 CH 2 ) n — (n=1-20), —(CH 2 ) m —C(O)NH—(CH 2 ) n — and —(OCH 2 CH 2 ) m —C(O)NH—(OCH 2 CH 2 H 2 ) n — (m and n can be the same or different and m and n are from about 1 to about 20), linkers containing amide groups like —R 1 —C(O)NH—R 2 —, monomethoxytrityl (MMT)-protected amino linker, monomethoxytrityl (MMT)-protected amino linker, trifluoroacetyl (TFA)-protected pentyl (C5) amino linker, and trifluoroacetyl (TFA)-protected hexyl (C6) amino linker. In some embodiments, the linker may be a complex linker comprising more than one functional group. Exemplary complex linkers may include those discussed in U.S. Pat. Nos. 7,553,958; 8,053,187; and 9,303,055; the contents of each of which are incorporated herein by reference in their entirety. Other non-cleavable linkers to solid supports may also include these disclosed in U.S. Pat. Nos. 8,569,515; and 8,853,132, the contents of each of which are incorporated herein by reference in their entirety. 
     In other embodiments, a spacer may be used to hold synthesized oligonucleotides away from the beads, therefore allow the short oligonucleotides to bind effectively to its complement on the end of the aptamer. As used herein, the term “spacer” refers to a chemical group connected to a linker that is used to extend the length of the linker moiety and as a site for initiating synthesis of a polymer chain. Examples of spacer include, but are not limited to, ethyleneglycol polymer, alkyl, oligonucleotides, peptides, and peptditomimetics. In other aspects, the spacer may be an anchor group that is attached to one end of the linker. The anchor group may be served as the site of oligonucleotide synthesis. At the end of oligonucleotide synthesis, a 5′DMT (4.4′-dimethoxytrityl) protecting group may be added to the last nucleoside, the role of which is to prevent interaction with the solid supports and between oligonucleotide polymers. 
     In some embodiments, low to moderate density of short oligonucleotides may be synthesized on the beads. The controlled loading allows for independent short oligonucleotides that have space to interact with the complementary sequence on the aptamer. The density of aptamer-complement complexes on the solid support may further be optimized to provide adequate signals when the conjugates are used in a detection assay. 
     In some embodiments, solid supports (e.g., magnetic beads and functionalized glasses) with synthesized oligonucleotides may be separated from the reaction suspension. For example, Magnet force may be used to pull down the beads from the reaction suspension. Separated beads are then read for fluorescent signals. 
     Target Allergens 
     Compositions, SPNs, SPN-complement complexes, magnetic particle conjugates and detection agents of the present invention may work as ligands for any target analyte. As stated below, the target analyte may be an allergen protein or variants thereof. In some embodiments, compositions, SPNs. SPN-complement complexes, magnetic particle conjugates and detection agents of the present invention may be designed to bind or associate with allergen proteins or other biomolecules which themselves associated with the allergen. 
     As used herein, the term “allergen” refers to a substance that can cause allergic reaction. An allergen is then a type of antigen that triggers an abnormally vigorous immune response in body. 
     Allergens include those from food products, the environment such as pollen, or animals such as a domestic pet dander. Food allergens include, but are not limited to proteins in legumes such as peanuts, peas, lentils and beans, tree nuts, wheat, milk, fish, egg white and sea food. Other allergens may be from the environment such as pollens, other animals (e.g., pet), pathogens and medicines. A comprehensive list of allergenic proteins from various sources is discussed below. 
     In some embodiments, allergens are food allergens. Examples of allergenic proteins associated with food include, but are not limited to, Brine shrimp (Art fr 5), Crab (Cha f 1), North Sea Shrimp (Cra c 1. Cra c 2, Cra c 4, Cra c 5, Cra c 6, Cra c 8), American lobster (Hom a 1, Hom a 3, Hom a 6), white shrimp (Lit v 1, Lit v 2, Lit v 3, Lit v4), giant freshwater prawn (Mac r 1), shrimp (Met e 1, Pen a 1, Pen i 1), northern shrimp (Pan b 1), spiny lobster (Pan s 1), black tiger shrimp (Pen m 1, Pen m 2, Pen m 3, Pen m 4, Pen m 6), narrow-clawed crayfish (Pon i 4, Pon i 7), blue swimmer crab (Por p 1), domestic cattle (Bos d 4, Bos d 5, Bos d 6, Bos d 7, Bos d 8, Bos d 9, Bos d 10, Bos d 11, Bos d 12), Atlantic herring (Clu h 1), common carp (Cyp c 1), Baltic cod (Gad c 1). Atlantic cod (Gad m 1, Gad m 2, Gad m 3), cod (Gad c 1), chicken (Gal d 1, Gal d 2, Gal d 3. Gal d 4, Gal d 5), Barramunda (Lat c 1), Lepidorhombus whiffiagonis (Lep w 1), chum salmon (One k 5), Atlantic salmon (Sal s 1, Sal s 2, Sal s 3) rainbow trout (One m 1), Mozambique tilapia (Ore m 4), edible frog (Ran e 1, Ran e 2), pacific pilchard (Sar sa 1), ocean perch (Seb m 1), yellowfin tuna (Thu a 1, Thu a 2, Thu a 3), swordfish (Xip g 1), abalone (Hal m 1), brown garden snail (Hel as 1), Squid (Tod p 1), pineapple (Ana c 1, Ana c 2), asparagus (Aspa o 1), barley (Hor v 12, Hor v 15, Hor v 16, Hor v 17, Hor v 20, Hor v 21), banana (Mus a 1, Mus a 2, Mus a 3, Mus a 4, Mus a 5), banana (Musxp1), rice (Ory s 12), rye (Sec c 20), wheat (Tri a 12, Tri a 14, Tri a 18, Tri a 19, Tri a 25, Tri a 26, Tri a 36, Tri a 37), maize (corn) (Zea m 14, Zea m 25), kiwi fruit (Act c1, Act c 2, Act c 5, Act c 8, Act c 10, Act d 1, Act d 2, Act d 3, Act d 4, Act d 5, Act d 6, Act d 7, Act d 8, Act d 9, Act d 10, Act d 1), cashew (Ana o 1, Ana o 2. Ana o 3), celery (Api g 1, Api g 2, Api g 3, Api g 4, Api g 5, Api g 6), peanut (Ara h 1, Ara h 2, Ara h 3, Ara h 4, Ara h 5, Ara h 6, Ara h 7, Ara h 8, Ara h 9, Ara h 10, Ara h 11, Ara h 12, Ara h 13), brazil nut (Ber e 1, Ber e 2), oriental mustard (Bra j 1), rapeseed (Bra n 1), cabbage (Bra o 3), turnip (Bra r 1, Bra r 2), bell pepper (Cap a 1w, Cap a 2), pecan (Car i 1, Car i 4), chestnut (Cas s 1, Cas s 5, Cas s 8, Cas s 9), lemon (Cit I 3), tangerine (Cit r 3), sweet orange (Cit s 1, Cit s 2, Cit s 3), Hazel (Cor a 1, Cor a 2, Cor a 8, Cor a 9, Cor a 11, Cor a 12, Cor a 13, Cor a 14), muskmelon (Cuc m 1, Cuc m 2, Cuc m 3), carrot (Dau c 1, Dau c 4, Dau c 5), common buckwheat (Fag e 2, Fag e 3), tartarian buckwheat (Fag t 2), strawberry (Fra a 1, Fra a 3, Fra a 4), soybean (Gly m 1, Gly m 2, Gly m 3, Gly m 4, Gly m 5, Gly m 6, Gly m 7, Gly m 8), sunflower (Hel a1, Hel a 2, Hel a 3), black walnut (Jug n 1, Jug n 2), English walnut (Jug r 1, Jug r 2, Jug r 3, Jug r 4), Cultivated lettuce (Lac s 1), Lentil (Len c 1, Len c 2, Len c 3), litchi (Lit c 1), narrow-leaved blue lupin (Lup an 1), apple (Mal d 1, Mal d 2, Mal d 3, Mal d 4), Cassava (Man e 5), mulberry (Mor n 3), avocado (Pers a 1), green bean (Pha v 3), pistachio (Pis v 1, Pis v 2, Pis v 3, Pis v 4, Pis v 5), pea (Pis s 1, Pis s 2), apricot (Pru ar 1, Pru ar 3), sweet cherry (Pru av 1, Pru av 2, Pru av 3, Pru av 4), European plum (Pru d 3), almond (Pru du 3, Pru du 4, Pru du 5, Pru du 6), peach (Pru p 1, Pru p 2, Pru p 3, Pru p 4, Pru p 7), pomegranate (Pun g 1), pear (Pyr c 1, Pyr c 3, Pyr c 4, Pyr c 5), castor bean (Ric c 1), red raspberry (Rub i 1, Rub i 3), Sesame (Ses i 1, Ses i 2, Ses i 3, Ses i 4, Ses i 5, Ses i 6, Ses i 7), yellow mustard (Sin a 1, Sin a 2, Sin a 3, Sin a 4), tomato (Sola I 1, Sola I 2, Sola I 3, Sola I 4), potato (Sola t 1, Sola t 2, Sola t 3, Sola t 4), Mung bean (Vig r 1, Vig r 2, Vig r 3, Vig r 4, Vig r 5, Vig r 6), grape (Vit v 1), Chinese date (Ziz m 1),  Anacardium occidentale  (Ana o 1.0101, Ana o 1.0102),  Apium graveolens  (Api g 1.0101, Api g 1.0201),  Daucus carota  (Dau c1.0101, Dau c1.0102, Dau c1.0103, Dau c1.0104, Dau c1.0105, Dau c1.0201),  Citrus sinensis  (Cit s3.0101, Cit s3.0102),  Glycine max  (Gly m1.0101, Gly m1.0102, Gly m3.0101, Gly m3.0102),  Lens culinaris  (Len c1.0101, Len c1.0102, Len c1.0103),  Pisum sativum  (Pis s1.0101, Pis s1.0102),  Lycopersicon sativum  (Lye e2.0101, Lye e2.0102),  Fragaria ananassa  (Fra a3.0101, Fra a3.0102, Fra a3.0201, Fr a3.0202, Fra a3.0203, Fra a3.0204, Fra a3.0301),  Malus domestica  (Mal d1.0101, Mal d1.0102, Mal d1.0103, Mal d1.0104, Mal d1.0105, Mal d1.0106, Mal d1.0107, Mal d1.0108, Mal d1.0109, Mal d1.0201, Mal d1.0202, Mal d1.0203, Mal d1.0204, Mal d1.0205, Mal d1.0206, Mal d1.0207, Mal d1.0208, Mal d1.0301, Mal d1.0302, Mal d1.0303, Mal d1.0304, Mal d1.0401, Mal d1.0402, Mal d1.0403, Mal d3.0101w, Mal d3.0102w, Mal d3.0201w, Mal d3.0202w, Mal d3.0203w, Mal d4.0101, Mal d4.0102, Mal d4.0201, Mal d4.0202, Mal d4.0301, Mal d4.0302),  Prunus avium  (Pru av1.0101, Pru av1.0201, Pru av 1.0202, Pru av1.0203), and  Prunus persica  (Pru p4.0101, Pru p4.0201); and any variants thereof. The names of allergens associated with food are systematically named and listed according to IUIS Allergen Nomenclature Sub-Committee (see, International Union of Immunological Societies Allergen Nomenclature Sub-Committee, List of isoallergens and variants.) 
     In addition to food allergens, SPNs, magnetic particle conjugates and compositions of the present invention may detect airborne particulates/allergens and other environmental allergens. Samples that contain allergens may be obtained from plants (e.g. weeds, grasses, trees, pollens), animals (e.g., allergens found in the dander, urine, saliva, blood or other bodily fluid of mammals such as cat, dog, cow, pig, sheep, horse, rabbit, rat, guinea pig, mouse and gerbil), fungi/mold, insects (e.g., stinging insects such as bee, wasp, and hornet and chirnomidae (non-biting midges), as well as other insects such as the housefly, fruit fly, sheep blow fly, screw worm fly, grain weevil, silkworm, honeybee, non-biting midge larvae, bee moth larvae, mealworm, cockroach and larvae of  Tenibrio molitor  beetle; spiders and mites such as the house dust mite), rubbers (e.g. latex), metals, chemicals (e.g. drugs, protein detergent additives) and autoallergens and human autoallergens (e.g. Hom s 1, Hom s 2, Hom s 3, Hom s 4, Hom s 5) (see, Allergen Nomenclature: International Union of Immunological Societies Allergen Nomenclature Sub-Committee, List of allergens and Allergen Nomenclature: International Union of Immunological Societies Allergen Nomenclature Sub-Committee, List of isoallergens and variants). 
     Examples of allergenic proteins from plants that can be detected using the compositions of the present invention include, but are not limited to, ash (Fra e 1), Japanese cypress (Cha o1, Cha o 2), sugi (Cry j 1, Cry j 2), cypress (Cup a 1), common cypress (Cup s 1, Cup s 3), mountain cedar (Jun a 1, Jun a 2, Jun a 3, Jun s 1), prickly juniper (Jun o 4), eastern red cedar (Jun v 1, Jun v 3), sweet vernal grass (Ant o 1), saffron crocus (Cro s 1, Cro s 2), Bermuda grass (Cyn d 1, Cyn d 7, Cyn d 12, Cyn d 15, Cyn d 22w, Cyn d 23, Cyn d 24), orchard grass (Dac g 1, Dac g 2, Dac g 3, Dac g 4, Dac g 5), meadow fescue (Fes p 4), velvet grass (Hol I 1, Hol I 5), barley (Hor v 1, Hor v 5), rye grass (Lol p 1, Lol p 2, Lol p 3, Lol p 4, Lol p 11), bahia grass (Pas n 1), canary grass (Pha a 1, Pha a 5), timothy (Phl p 1, Phi p 2, Phi p 4, Phi p 5, Phi p 6, Phl p 7, Phl p 11, Phl p 12, Phi p 13), date palm (Pho d 2), Kentucky blue grass (Poa p 1, Poa p 5), rye (Sec c 1, Sec c 5, Sec c 38), Johnson grass (Sor h 1), wheat (Tri a 15, Tri a 21, Tri a 27, Tri a 28, Tri a29, Tri a30, Tri a 31, Tri a32, Tri a 33, Tri a 34, Tri a 35, Tri a 39), maize (Zea m 1, Zea m 12), alder (Aln g 1, Aln g 4), redroot pigweed (Ama r 2), short ragweed (Amb a 1, Amb a 2, Amb a 3, Amb a 4, Amb a 5, Amb a 6, Amb a 7, Amb a 8, Amb a 9, Amb a 10, Amb a 11), western ragweed (Amb p 5), giant ragweed (Amb t 5), mugwort (Art v 1, Art v 2, Art v 3, Art v 4, Art v 5, Art v 6), sugar beet (Beta v 1, beta v 2), European white birch (Bet v 1, Bet v 2, Bet v 3, Bet v 4, Bet v 6, Bet v 7), turnip (Bra r 5), hornbeam (Car b 1), chestnut (Cas s 1), rosy periwinkle (Cat r 1), lamb&#39;s-quarters, pigweed (Che a 1, Che a 2, Che a 3), Arabian coffee (Cof a 1, Cof a 2, Cof a 3), Hazel (Cor a 6, Cor a 10), Hazel nut (Cor a1.04, Cor a2, Cor a8), European beech (Fag s 1), ash (Fra e 1), sunflower (Hel a 1, Hel a 2), para rubber tree (Hev b 1, Hev b 2, Hev b 3, Hev b 4, Hev b 5, Hev b 6, Hev b 7, Hev b 8, Hev b 9, Hev b 10, Hev b 11, Hev b 12, Hev b 13, Hev b 14). Japanese hop (Hum j 1), privet (Lig v 1),  Mercurialis annua  (Mer a 1), olive (Ole e 1, Ole e 2, Ole e 3, Ole e 4, Ole e 5, Ole e 6, Ole e 7, Ole e 8, Ole e 9, Ole e 10, Ole e 11), European hophornbeam (Ost c 1),  Parietaria judaica  (Par j 1, Par j 2, Par j 3, Par j 4),  Parietaria officinalis  (Par o 1),  Plantago lanceolata  (Pal I 1), London plane tree (Pla a 1, Pla a 2, Pla a 3),  Platanus orientalis  (Pla or 1, Pla or 2, Pla or 3), white oak (Que a 1), Russian thistle (Sal k 1, Sal k 2, Sal k 3, Sal k 4, Sal k 5), tomato (Sola I 5), Lilac (Syr v 1, Syr v 5), Russian-thistle (Sal k 1), English plantain (Pla 11),  Ambrosia artemisiifolia  (Amb a8.0101, Amb a8.0102, Amb a9.0101, Amb a9.0102),  Plantago lanceolata  (Pla 11.0101, Pla 11.0102, Pla 11.0103),  Parietaria judaica  (Par j 3.0102),  Cynodon dactylon  (Cyn d1.0101, Cyn d1.0102, Cyn d1.0103, Cyn d1.0104, Cyn d1.0105, Cyn d1.0106, Cyn d1.0107, Cyn d1.0201, Cyn d1.0202, Cyn d1.0203, Cyn d1.0204),  Holcus lanatus  (Hol 11.0101, Hol 11.0102),  Lolium perenne  (Phl p1.0101, Phl p1.0102, Phl p4.0101, Phl p4.0201, Phl p5.0101, Phl p5.0102, Phl p5.0103, Phi p5.0104, Phl p5.0105, Phl p5.0106, Phl p5.0107, Phl p5.0108, Phl p5.0201, Phl p5.0202),  Secale cereale  (Sec c20.0101. Sec c20.0201),  Betula Verrucosa  (Bet v1.0101, Bet v1.0102, Bet v 1.0103, Bet v 1.0201, Bet v 1.0301, Bet v1.0401, Bet v 1.0402, Bet v 1.0501, Bet v 1.0601, Bet v 1.0602, Bet v1.0701, Bet v1.0801, Bet v1.0901, Bet v1.1001, Bet v1.1101, Bet v1.1201, Bet v 1.1301, Bet v1.1401, Bet v1.1402, Bet v1.1501, Bet v1.1502, Bet v1.1601, Bet v1.1701, Bet v 1.1801, Bet v1.1901, Bet v1.2001, Bet v1.2101, Bet v1.2201, Bet v1.2301, Bet v1.2401, Bet v 1.2501, Bet v1.2601, Bet v1.2701, Bet v1.2801, Bet v1.2901, Bet v1.3001, Bet v1.3101, Bet v 6.0101, Bet v6.0102),  Carpinus betulus  (Car b1.0101, Car b1.0102, Car b1.0103, Car b1.0104, Car b1.0105, Car b1.0106, Car b1.0106, Car b1.0106, Car b1.0106, Car b1.0107, Car b1.0107, Car b1.0108, Car b1.0201, Car b1.0301, Car b1.0302),  Corylus avellana  (Cor a1.0101, Cor a1.0102, Cor a1.0103, Cor a1.0104, Cor a1.0201, Cor a1.0301. Cor a1.0401, Cor a1.0402, Cor a1.0403, Cor a1.0404),  Ligustrum vulgare  (Syr v1.0101, Syr v1.0102, Syr v1.0103),  Cryptomeria japonica  (Cry j2.0101, Cry j2.0102), and  Cupressus sempervirens  (Cup s1.0101, Cup s1.0102, Cup s1.0103, Cup s1.0104, Cup s1.0105), and any variants thereof. 
     Lupin is an herbaceous plant of the leguminous family belonging to the genus  Lupinus . In Europe, lupin flour and seeds are widely used in bread, cookies, pastry, pasta, sauces, as well as in beverages as a substitute for milk or soy, and in gluten-free foods. The International Union of Immunological Societies (IUIS) allergen nomenclature subcommittee recently designated β-conglutin as the Lup an 1 allergen. (Nadal, et al., (2012)  PLoS one,  7(4): e35253), and more recently, a high-affinity 11-mer DNA aptamer against Lup an 1 (β-conglutin) was reported (Nadal, et al., (2013),  Anal. Bioanal. Chem.  405:9343-9349). 
     Examples of allergenic proteins from mites that can be detected using the compositions of the present invention include, but are not limited to, mite (Blo t 1, Blo t 3. Blo t 4, Blo t 5, Blo t 6, Blo t 10, Blo t 11, Blo t 12, Blo t 13, Blo t 19, Blot t 21); American house dust mite (Der f 1, Der f 2, Der f 3, Der f 7, Der f 10, Der f 11, Der f 13, Der f 14, Der f 15, Der f 16, Der f 17, Der f 18, Der f 22, Der f 24);  Dermatophagoides microceras  (house dust mite) (Der m 1); European house dust mite (Der p 1, Der p 2, Der p 3, Der p 4, Der p 5, Der p 6, Der p 7, Der p 8, Der p 9, Der p 10, Der p 11, Der p 14, Der p 15, Der p 20, Der p 21, Der p 23);  Euroglyphus maynei  (House dust mite) (Eur m 2, Eur m 2, Eur m 3, Eur m 4, Eur m 14); storage mite (Aca s 13, Gly d 2, Lep d 2, Lep d 5, Lep d 7, Lep d 10, Lep d 13, Tyr p 2, Tyr p 3, Tyr p 10, Tyr p 13. Tyr p 24),  Dermatophagoides farinae  (Der f1.0101, Der f1.0102, Der f1.0103, Der f1.0104, Der f1.0105, Der f2.0101, Der f2.0102, Der f2.0103, Der f2.0104, Der f2.0105, Der f2.0106, Der f2.0107, Der f2.0108, Der f2.0109, Der f2.0110, Der f2.0111, Der f2.0112, Der f2.0113, Der f2.0114, Der f2.0115, Der f2.0116, Der f2.0117),  Dermatophagoides pteronyssinus  (Der p 1.0101, Der p 1.0102, Der p 1.0103, Der p 1.0104, Der p1.0105, Der p1.0106, Der p1.0107, Der p1.0108, Der p1.0109, Der p1.0110, Der p1.0111. Der p1.0112, Der p1.0113, Der p1.0114, Der p1.0115, Der p1.0116, Der p1.0117, Der p1.0118, Der p1.0119, Der p1.0120, Der p1.0121, Der p1.0122, Der p1.0123, Der p2.0101, Der p2.0102, Der p2.0103, Der p2.0104, Der p2.0105, Der p2.0106, Der p2.0107, Der p2.0108, Der p2.0109, Der p2.0110, Der p2.0111, Der p2.0112, Der p2.0113),  Euroglyphus maynei  (Eur m2.0101, Eur m2.0102),  Lepidoglyphus destructor  (Lep d2.0101, Lep d2.0101, Lep d2.0101, Lep d2.0102, Lep d2.0201, Lep d2.020) and  Glycphagus domesticus  (Gly d2.0101, Gly d2.0201); and any variants thereof. 
     Examples of allergenic proteins from animals that can be detected using the compositions of the present invention include, but are not limited to, domestic cattle (Bos d 2, Bos d 3, Bos d 4, Bos d 5, Bos d 6, Bos d 7, Bos d 8), dog (Can f 1, Can f 2, Can f3, Can f 4, Can f 5, Can f 6), domestic horse (Equ c 1, Equ c 2, Equ c 3, Equ c 4, Equ c 5), cat (Fel d 1, Fel d 2, Fel d 3, Fel d 4, Fel d 5w, Fel d 6w, Fel d 7, Fel d 8), mouse (Mus m 1), guinea pig (Cav p 1, Cav p 2, Cav p 3, Cav p 4, Cav p 6), rabbit (Ory c 1, Ory c 3, Ory c 4) rat (Rat n 1),  Bos domesticus  (Bos d 2.0101, Bos d 2.0102, Bos d 2.0103) and  Equus caballus  (Equ c2.0101, Equ c 2.0102); and any variants thereof 
     Examples of allergenic proteins from insects that can be detected using the compositions of the present invention include, but are not limited to, yellow fever mosquito (Aed a 1, Aed a 2, Aed a 3). Eastern hive bee (Api c 1), giant honeybee (Api d 1), honey bee (Api m 1, Api m 2, Api m 3, Api m 4, Api m 5, Api m 6, Api m 7, Api m 8, Api m 9, Api m 10, Api m 11, Api m 12), pigeon tick (Arg r 1), German cockroach (Bla g 1, Bla g 2, Bla g 3, Bla g 4, Bla g 5, Bla g 6, Bla g 7, Bla g 8, Bla g 11), bumble bee (Bom p 1, Bom p 4, Bom t 1, Bom t 4), silk moth (Bomb m 1), midge (Chi k 10, Chi t 1, Chi t 1.01, Chi t 2, Chit 2. 0101, Chi t 2. 0102, Chit 3, Chit 4, Chit 5, Chit 6, Chi t 6.01, Chit 7, Chi t 8, Chit 9), cat flea (Cte f 1, Cte f 2, Cte f 3), yellow hornet (Dol a 5), white face hornet (Dol m 1, Dol m 2, Dol m 5), biting midge (For t 1, For t 2), Savannah Tsetse fly (Glo m 5), Asian ladybeetle (Har a 1, Har a 2), silverfish (Lep s 1), booklouse (Lip b 1), Australian jumper ant (Myr p 1, Myr p 2, Myr p 3), American cockroach (Per a 1, Per a 3, Per a 6, Per a 7, Per a 9, Per a 10), Indian meal moth (Plo i 1, Plo i 2), wasp (Pol a 1, Pol a 2, Pol a 5, Pole 1, Pol e 4, Pol e 5, Pol f 5, Pol g 1, Pol g 5, Pol m 5, Poly p 1, Poly s 5, Ves vi 5), Mediterranean paper wasp (Pol d 1, Pol d 4, Pol d 5), tropical fire ant (Sol g 2, Sol g 3, Sol g 4),  Solenopsis invicta  (red imported fire ant) (Sol I 1, Sol I 2, Sol I 3, Sol I 4), black fire ant (Sol r 2, Sol r 3), Brazilian fire ant (Sol s 2, Sol s 3), horsefly (Tab y 1, Tab y 2, Tab y 5), pine processionary moth (Tha p 1, Tha p 2), California kissing bug (Tria p 1), European hornet (Vesp c 1, Vesp c 5),  Vespa magnifica  (hornet) (Vesp ma 2, Vesp ma 5),  Vespa mandarinia  (Giant asian hornet) (Vesp m1, Vesp m 5), yellow jacket (Ves f 5, Ves g 5, Ves m 1, Ves m 2, Ves m 5),  Vespula germanica  (yellow jacket) (Ves p 5),  Vespula squamosa  (Yellow jacket) (Ves s 1, Ve s s5),  Vespula vulgaris  (Yellow jacket) (Ves v 1, Ves v 2, Ves v 3, Ves v 4, Ves v 5, Ves v 6),  Blattella germanica  (Bla g 1.0101, Bla g 1.0102, Bla g 1.0103, Bla g 1.02, Bla g 6.0101, Bla g 6.0201, Bla g 6.0301),  Periplaneta Americana  (Per a1.0101, Per a1.0102, Per a1.0103, Per a1.0104, Per a1.02, Per a3.01, Per a3.0201, Per a3.0202, Per a3.0203, Per a7.0101, Per a7.0102),  Vespa crabo  (Ves pc 5.0101, Ves pc 5.0101),  Vespa mandarina  (Vesp m 1.01, Vesp m 1.02); and any variants thereof. 
     Examples of allergenic proteins from fungi/mold that can be detected using the signaling polynucleotides and assays of the present invention include, but are not limited to,  Alternaria alternata  (Alternaria rot fungus) (Alt a 1, Alt a 3, Alt a 4, Alt a 5, Alt a 6, Alt a 7, Alt a 8, Alt a 10, Alt a 12, Alt a 13),  Aspergillus flavus  (fungus) (Asp fl 13),  Aspergillus fumigatus  (fungus) (Asp f 1, Asp f 2, Asp f 3, Asp f 4, Asp f 5, Asp f 6, Asp f 7, Asp f 8, Asp f9, Asp f 10, Asp f 11, Asp f 12, Asp f 13, Asp f 15, Asp f 16, Asp f 17, Asp f 18, Asp f22, Asp f 23, Asp f 27, Asp f 28, Asp f 29, Asp f 34),  Aspergillus niger  (Asp n 14, Asp n 18, Asp n 25),  Aspergillus oryzae  (Asp o 13, Asp o 21),  Aspergillus versicolor  (Asp v 13),  Candida albicans  (Yeast) (Cand a 1, Cand a 3),  Candida boidinii  (Yeast) (Cand b 2),  Cladosporium cladosporioides  (Cla c 9, Cla c 14),  Cladosporium herbarum  (Cla h 2, Cla h 5, Cla h 6, Cla h 7, Cla h 8, Cla h 9, Cla h 10, Cla h 12),  Curvularia lunata  (Synonym:  Cochliobolus lunatus ) (Cur I 1, Cur I 2, Cur I 3, Cur I 4),  Epicoccum purpurascens  (Soil fungus) (Epi p 1),  Fusarium culmorum  (N.A.) (Fus c 1, Fus c 2).  Fusarium proliferatum  (Fus p 4),  Penicillium brevicompactum  (Pen b 13, Pen b 26),  Penicillium chrysogenum  (Pen ch 13, Pen ch 18, Pen ch 20, Pen ch 31, Pen ch 33, Pen ch 35),  Penicillium citrinum  (Pen c 3. Pen c 13, Pen c 19, Pen c 22, Pen c 24, Pen c 30. Pen c 32),  Penicillium crustosum  (Pen cr 26),  Penicillium oxalicum  (Pen o 18),  Stachybotrys chartarum  (Sta c 3),  Trichophyton rubrum  (Tri r 2, Tri r 4),  Trichophyton tonsurans  (Tri t 1, Tri t 4),  Psilocybe cubensis  (Psi c 1, Psi c 2), Shaggy cap (Cop c 1, Cop c 2, Cop c 3, Cop c 5, Cop c 7),  Rhodotorula mucilaginosa  (Rho m 1, Rho m 2),  Malassezia furfur  (Malaf2, Malaf3, Malaf4),  Malassezia sympodialis  (Malas1, Malas5, Malas6, Malas7, Malas8, Malas9, Malas10, Malas11, Malas12, Malas13) and  Alternaria alternate  (Alt a1.0101, Alt a1.0102); and any variants thereof. 
     Examples of additional allergens include, but are not limited to, Nematode (Ani s 1, Ani s 2, Ani s 3, Ani s 4), worm (Asc s 1), soft coral (Den n 1), rubber (Latex) (Hev b 1, Hev b 2, Hev b 3, Hev b 5, Hev b 6, Hev b 7, Hev b 8, Hev b 9, Hev b 10, Hev b 11, Hev b 12, Hev b 13), obeche (Trip s 1) and Heveabrasiliensis (Hev b6.01, Hev b6.0201, Hev b6.0202, Hev b6.03, Hev b8.0101, Hev b8.0102, Hev b8.0201, Hev b8.0202, Hev b8.0203, Hev b8.0204, Hev b10.0101, Hev b10.0102, Hev b10.0103, Hev b11.0101, Hev b11.0102); and any variants thereof. 
     Table 5 provides a list of non-limiting examples of allergenic proteins from various species. In the table, in addition to the species and allergen name, provided are the biochemical name of the allergenic protein and the unique identification numbers from GenBank or UniProt databases. In some embodiments, SPNs, magnetic particle conjugates and compositions of the present invention may be used to detect any of the allergenic proteins listed in Table 5, or any variant, fragment or antigenic portion thereof. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Allergen proteins 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                 Food 
                   
                   
                   
                 SEQ ID 
               
               
                 Species 
                 Allergen 
                 Allergen 
                 Biochemical name 
                 GenBank 
                 Uniprot 
                 NO 
               
               
                   
               
            
           
           
               
            
               
                 Animalia Arthropoda 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   Acarus siro  (Storage 
                   Aca s  13 
                 No 
                 Fatty acid-binding protein 
                 ABL09307.1 
                 B0KZJ6 
                 805 
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  1 
                 No 
                 Apyrase 
                 AAC37218 
                 P50635 
                 806 
               
               
                 fever mosquito) 
                   
                   
                   
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  2 
                 No 
                 Salivary D7 protein 
                 AAA29347 
                 P18153 
                 807 
               
               
                 fever mosquito) 
                   
                   
                   
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  3 
                 No 
                 Undefined 30 kDa salivary 
                 AAB58417 
                 O01949 
                 808 
               
               
                 fever mosquito) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  4 
                 No 
                 α-glucosidase 
                 P13080 
                 / 
                 809 
               
               
                 fever mosquito) 
                   
                   
                   
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  5 
                 No 
                 Sarcoplasmic Ca +  (EF- 
                 XP_001653462.1 
                 Q16XK7 
                 810 
               
               
                 fever mosquito) 
                   
                   
                 hand) binding protein 
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  6 
                 No 
                 Porin3 
                 XP_001654143.1 
                 Q1HR57 
                 811 
               
               
                 fever mosquito) 
                   
                   
                   
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  7 
                 No 
                 Undefined protein 
                 XP_001654291.1 
                 Q16TN9 
                 812 
               
               
                 fever mosquito) 
                   
                   
                   
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  8 
                 No 
                 Heat Shock cognate 
                 ABF18258.1 
                 Q1HR69 
                 813 
               
               
                 fever mosquito) 
                   
                   
                 protein-70 
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                 
                   Aed a 
                 
                 No 
                 Tropomyosin 
                 XP_001655954.1 
                 Q17H75 
                 814 
               
               
                 fever mosquito) 
                 10.0101 
                   
                   
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                 
                   Aed a 
                 
                 No 
                 Tropomyosin 
                 XP_001655948.1 
                 Q17H80 
                 815 
               
               
                 fever mosquito) 
                 10.0102 
                   
                   
                   
                   
                   
               
               
                   Aedes aegypti  (Yellow 
                   Aed a  11 
                 No 
                 Lysosomal aspartic 
                 XP_001657556.1 
                 Q03168 
                 816 
               
               
                 fever mosquito) 
                   
                   
                 protease 
                   
                   
                   
               
               
                   Apis cerana  (Asiatic 
                   Api c  1 
                 No 
                 Phospholipase A2 
                 AAK09361 
                 Q9BMK4 
                 817 
               
               
                 honey bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis dorsata  (Giant 
                   Api d  1 
                 No 
                 Phospholipase A2 
                 Q7M4I5 
                 Q7M4I5 
                 818 
               
               
                 honeybee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  1 
                 No 
                 Phospholipase A2 
                 CAA34681 
                 P00630 
                 819 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  2 
                 No 
                 Hyaluronidase 
                 AAA27730 
                 Q08169 
                 820 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  3 
                 No 
                 Acid phosphatase 
                 AAY57281 
                 Q4TUB9 
                 821 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  4 
                 No 
                 Melittin 
                 CAA26038 
                 P01501 
                 822 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  5 
                 No 
                 Dipeptidylpeptidase IV 
                 NP_001119715 
                 B2D0J4 
                 823 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  6 
                 No 
                 / 
                 NP_001035360 
                 Q27SJ8 
                 824 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  7 
                 No 
                 CUB serine protease 
                 AAN02286 
                 Q8MQS8 
                 825 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  8 
                 No 
                 Carboxylesterase VI 
                 ACB70231 
                 B2D0J5 
                 826 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  9 
                 No 
                 Serine carboxypeptidase 
                 ACN71203 
                 C9WMM5 
                 827 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  10 
                 No 
                 Icarapin variant 2, 
                 ABF21078 
                 Q1HHN7 
                 828 
               
               
                 bee) 
                   
                   
                 carbohydrate-rich protein 
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                 
                   Api m 
                 
                 No 
                 Major royal jelly protein 8, 
                 NP_001011564 
                 B3GM11 
                 829 
               
               
                 bee) 
                 11.0101 
                   
                 variant 1 
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                 
                   Api m 
                 
                 No 
                 Major royal jelly protein 8, 
                 AAY21180 
                 Q4ZJX1 
                 830 
               
               
                 bee) 
                 11.0201 
                   
                 variant 2 
                   
                   
                   
               
               
                   Apis mellifera  (Honey 
                   Api m  12 
                 No 
                 Vitellogenin 
                 CAD56944 
                 Q868N5 
                 831 
               
               
                 bee) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Archaeopotamobius 
                 
                   Arc s  8 
                 Yes 
                 Triosephosphate isomerase 
                 CAD29196 
                 Q8T5G9 
                 832 
               
               
                   sibiriensis  (Crustacean 
                   
                   
                   
                   
                   
                   
               
               
                 species decapod) 
                   
                   
                   
                   
                   
                   
               
               
                   Argas reflexus  (Pigeon 
                   Arg r  1 
                 No 
                 / 
                 CAG26895 
                 Q5GQ85 
                 833 
               
               
                 tick) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Artemia franciscana 
                 
                   Art fr  5 
                 Yes 
                 Myosin, light chain 1 
                 ABS19977 
                 A7L499 
                 834 
               
               
                 (Brine shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                 
                   Bla g 
                 
                 No 
                 / 
                 AAD13530 
                 Q9UAM5 
                 835 
               
               
                 (German cockroach) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                 
                   Bla g 
                 
                 No 
                 / 
                 AAD13531 
                 O96522 
                 836 
               
               
                 (German cockroach) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                   Bla g  2 
                 No 
                 Aspartic protease 
                 AAA86744 
                 P54958 
                 837 
               
               
                 (German cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                   Bla g  3 
                 No 
                 Hemocyanin 
                 ACY40651 
                 D0VNY7 
                 838 
               
               
                 (German cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                   Bla g  4 
                 No 
                 Calycin 
                 AAA87851 
                 P54962 
                 839 
               
               
                 (German cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                   Bla g  5 
                 No 
                 Glutathione S-transferase 
                 AAB72147 
                 O18598 
                 840 
               
               
                 (German cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                 
                   Bla g 
                 
                 No 
                 Troponin C, isoform 1 
                 ABB89296 
                 Q1A7B3 
                 841 
               
               
                 (German cockroach) 
                 6.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                 
                   Bla g 
                 
                 No 
                 Troponin C, isoform 2 
                 ABB89297 
                 Q1A7B2 
                 842 
               
               
                 (German cockroach) 
                 6.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                 
                   Bla g 
                 
                 No 
                 Troponin C, isoform 3 
                 ABB89298 
                 Q1A7B1 
                 843 
               
               
                 (German cockroach) 
                 6.0301 
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                   Bla g  7 
                 No 
                 Tropomyosin 
                 AAF72534 
                 Q9NG56 
                 844 
               
               
                 (German cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                   Bla g  8 
                 No 
                 Myosin, light chain 
                 ABD47458 
                 A0ERA8 
                 845 
               
               
                 (German cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                   Bla g  9 
                 No 
                 Arginine kinase 
                 ABC86902 
                 / 
                 846 
               
               
                 (German cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blattella germanica 
                 
                   Bla g  11 
                 No 
                 Alpha-amylase 
                 ABC68516 
                 Q2L7A6 
                 847 
               
               
                 (German cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                 
                   Blo t 
                 
                 No 
                 Cysteine protease, isoform 
                 AK58415 
                 Q95PJ4 
                 848 
               
               
                 (Storage mite) 
                 1.0101 
                   
                 1 
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                 
                   Blo t 
                 
                 No 
                 Cysteine protease, isoform 
                 AAQ24541 
                 A1KXI0 
                 849 
               
               
                 (Storage mite) 
                 1.0201 
                   
                 2 
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                 
                   Blo t 
                 
                 No 
                 / 
                 AAQ73483 
                 Q1M2P1 
                 850 
               
               
                 (Storage mite) 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                 
                   Blo t 
                 
                 No 
                 / 
                 AAQ73482 
                 Q1M2P2 
                 851 
               
               
                 (Storage mite) 
                 2.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                 
                   Blo t 
                 
                 No 
                 / 
                 AAQ73481 
                 Q1M2P3 
                 852 
               
               
                 (Storage mite) 
                 2.0103 
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  3 
                 No 
                 Trypsin 
                 AAM10779 
                 Q8I916 
                 853 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  4 
                 No 
                 Alpha amylase 
                 AAQ24543 
                 A1KXI2 
                 854 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  5 
                 No 
                 / 
                 AAD10850 
                 O96870 
                 855 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  6 
                 No 
                 Chymotrypsin 
                 AAQ24544 
                 A1KXI3 
                 856 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  7 
                 No 
                 Bactericidal permeability- 
                 ASX95438 
                 / 
                 857 
               
               
                 (Storage mite) 
                   
                   
                 increasing like protein 
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  8 
                 No 
                 Glutathione S-transferase 
                 ACV04860 
                 C8CGT7 
                 858 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  10 
                 No 
                 Tropomyosin 
                 ABU97466 
                 A7XZI4 
                 859 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  11 
                 No 
                 Paramyosin 
                 AAM83103 
                 Q8MUF6 
                 860 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  12 
                 No 
                 / 
                 AAA78904 
                 Q17282 
                 861 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  13 
                 No 
                 Fatty acid-binding protein 
                 AAC80579 
                 Q17284 
                 862 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  19 
                 No 
                 Anti-microbial peptide 
                 AHG97583 
                 W5RZ24 
                 863 
               
               
                 (Storage mite) 
                   
                   
                 homologue 
                   
                   
                   
               
               
                 
                   Blomia tropicalis 
                 
                   Blo t  21 
                 No 
                 / 
                 AAX34047 
                 A7IZE9 
                 864 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bombus 
                 
                   Bom p  1 
                 No 
                 Phospholipase A2 
                 Q7M4I6 
                 Q7M4I6 
                 865 
               
               
                 
                   pennsylvanicus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (Bumble bee) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bombus 
                 
                   Bom p  4 
                 No 
                 Protease 
                 Q7M4I3 
                 Q7M4I3 
                 866 
               
               
                 
                   pennsylvanicus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (Bumble bee) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bombus terrestris 
                 
                   Bom t  1 
                 No 
                 Phospholipase A2 
                 P82971 
                 P82971 
                 867 
               
               
                 (Bumble bee) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bombus terrestris 
                 
                   Bom t  4 
                 No 
                 Protease 
                 P0CH88 
                 P0CH88 
                 868 
               
               
                 (Bumble bee) 
                   
                   
                   
                   
                   
                   
               
               
                   Bombyx mori  (Silk 
                 
                   Bomb m 
                 
                 Yes 
                 Arginine kinase 
                 ABB88514 
                 Q2F5T5 
                 869 
               
               
                 moth) 
                 1 
                   
                   
                   
                   
                   
               
               
                 
                   Charybdis feriatus 
                 
                   Cha f  1 
                 Yes 
                 Tropomyosin 
                 AAF35431 
                 Q9N2R3 
                 870 
               
               
                 (Crab) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Chironomus kiiensis 
                 
                   Chi k  10 
                 No 
                 Tropomyosin 
                 CAA09938 
                 O96764 
                 871 
               
               
                 (Midge) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, component 
                 AAA28249 
                 P02229 
                 872 
               
               
                   thummi  (Midge) 
                 1.0101 
                   
                 III 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, component 
                 AA25438 
                 P02230 
                 873 
               
               
                   thummi  (Midge) 
                 1.0201 
                   
                 IV 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, component 
                 AAA80189 
                 P02221 
                 874 
               
               
                   thummi  (Midge) 
                 2.0101 
                   
                 I/IA 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, component 
                 / 
                 P02221 
                 874 
               
               
                   thummi  (Midge) 
                 2.0201 
                   
                 I/IA 
                   
                 (variant 
                   
               
               
                   
                   
                   
                   
                   
                 A113T) 
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAB58932 
                 P02222 
                 875 
               
               
                   thummi  (Midge) 
                 3.0101 
                   
                 II-beta 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAA69813 
                 P02224 
                 876 
               
               
                   thummi  (Midge) 
                 3.0201 
                   
                 VI 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAB58930 
                 P02226 
                 877 
               
               
                   thummi  (Midge) 
                 3.0301 
                   
                 VIIA 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAB58931 
                 P02223 
                 878 
               
               
                   thummi  (Midge) 
                 3.0401 
                   
                 IX 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAA28260 
                 P12548 
                 879 
               
               
                   thummi  (Midge) 
                 3.0501 
                   
                 VIIB-3 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAA85491 
                 P84296 
                 880 
               
               
                   thummi  (Midge) 
                 3.0601 
                   
                 VIIB-4 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAA69815 
                 P84298 
                 881 
               
               
                   thummi  (Midge) 
                 3.0701 
                   
                 VIIB-9 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAA85486 
                 P12549 
                 882 
               
               
                   thummi  (Midge) 
                 3.0702 
                   
                 VIIB-6 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 AAA85485 
                 P12550 
                 883 
               
               
                   thummi  (Midge) 
                 3.0801 
                   
                 VIIB-7 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                 
                   Chi t 
                 
                 No 
                 Hemoglobin, components 
                 P02227 
                 P02227 
                 884 
               
               
                   thummi  (Midge) 
                 3.0901 
                   
                 VIII 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                   Chi t  4 
                 No 
                 Hemoglobin, component 
                 P02231 
                 P02231 
                 885 
               
               
                   thummi  (Midge) 
                   
                   
                 IIIA 
                   
                   
                   
               
               
                 
                   Chironomus thummi 
                 
                   Chi t  9 
                 No 
                 Hemoglobin, component X 
                 P02228 
                 P02228 
                 886 
               
               
                   thummi  (Midge) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Chortoglyphus arcuatus 
                 
                   Cho a  10 
                 No 
                 Tropomyosin 
                 AEX31649 
                 / 
                 887 
               
               
                 (Storage mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Crangon crangon 
                 
                   Cra c  1 
                 Yes 
                 Tropomyosin 
                 ACR43473 
                 D7F1J4 
                 888 
               
               
                 (North Sea shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Crangon crangon 
                 
                   Cra c  2 
                 Yes 
                 Arginine kinase 
                 ACR43474 
                 D7F1J5 
                 889 
               
               
                 (North Sea shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Crangon crangon 
                 
                   Cra c  4 
                 Yes 
                 Sarcoplasmic calcium- 
                 ACR43475 
                 D7F1P9 
                 890 
               
               
                 (North Sea shrimp) 
                   
                   
                 binding protein 
                   
                   
                   
               
               
                 
                   Crangon crangon 
                 
                   Cra c  5 
                 Yes 
                 Myosin, light chain 1 
                 ACR43477 
                 D7F1Q1 
                 891 
               
               
                 (North Sea shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Crangon crangon 
                 
                   Cra c  6 
                 Yes 
                 Troponin C 
                 ACR43478 
                 D7F1Q2 
                 892 
               
               
                 (North Sea shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Crangon crangon 
                 
                   Cra c  8 
                 Yes 
                 Triosephosphate isomerase 
                 ACR43476 
                 D7F1Q0 
                 893 
               
               
                 (North Sea shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ctenocephalides felis 
                 
                   Cte f  1 
                 No 
                 Salivary antigen 1 
                 AAC69105 
                 Q94424 
                 894 
               
               
                 (Cat flea) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ctenocephalides felis 
                 
                   Cte f  2 
                 No 
                 Salivary allergen 2 
                 AAF65314 
                 Q9NH66 
                 895 
               
               
                 (Cat flea) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ctenocephalides felis 
                 
                   Cte f  3 
                 No 
                 Salivary allergen 3 
                 / 
                 / 
                 / 
               
               
                 (Cat flea) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Cysteine protease 
                 BAC53948 
                 Q58A71 
                 896 
               
               
                   farinae  (American 
                 1.0101 
                   
                 (preproenzyme) 
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Cysteine protease variant 
                 / 
                 Q3HWZ4 
                 897 
               
               
                   farinae  (American 
                 1.0102 
                   
                   
                   
                 (variant) 
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Cysteine protease-1 
                 BAC53948 
                 P16311 
                 898 
               
               
                   farinae  (American 
                 1.0106 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Cysteine protease 
                 ABL84749 
                 A1YW11 
                 899 
               
               
                   farinae  (American 
                 1.0108 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Cysteine protease 
                 ABL84750 
                 A1YW12 
                 900 
               
               
                   farinae  (American 
                 1.0109 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Vitellogenin 
                 ABL84751 
                 A1YW13 
                 901 
               
               
                   farinae  (American 
                 1.0110 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 BAA01239 
                 Q00855 
                 902 
               
               
                   farinae  (American 
                 2.0101 
                   
                   
                   
                 (variant) 
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 BAA01240 
                 Q00855 
                 903 
               
               
                   farinae  (American 
                 2.0102 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 BAA01241 
                 Q00855 
                 903 
               
               
                   farinae  (American 
                 2.0103 
                   
                   
                   
                 (variant) 
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 AAL47677 
                 Q8WQK5 
                 904 
               
               
                   farinae  (American 
                 2.0105 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 CAI05848 
                 Q5TIW2 
                 905 
               
               
                   farinae  (American 
                 2.0106 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 CAI05849 
                 Q5TIW1 
                 906 
               
               
                   farinae  (American 
                 2.0107 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 CAI05850 
                 Q5TIW0 
                 907 
               
               
                   farinae  (American 
                 2.0108 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 ABA39438 
                 Q3HWZ2 
                 908 
               
               
                   farinae  (American 
                 2.0109 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 AAP35073 
                 A1KXH0 
                 909 
               
               
                   farinae  (American 
                 2.0112 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 NPC2 family 
                 / 
                 A3F5F1 
                 910 
               
               
                   farinae  (American 
                 2.0116 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  3 
                 No 
                 Trypsin 
                 BAA09920 
                 P49275 
                 911 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  4 
                 No 
                 alpha-amylase 
                 AHX03180 
                 / 
                 912 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  6 
                 No 
                 Chymotrypsin 
                 AAF28423 
                 P49276 
                 913 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  7 
                 No 
                 Bactericidal permeability- 
                 AAB35977 
                 Q26456 
                 914 
               
               
                   farinae  (American 
                   
                   
                 increasing like protein 
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  8 
                 No 
                 Glutathione S-transferase 
                 AGC56215 
                 / 
                 915 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  10 
                 No 
                 Tropomyosin 
                 BAA04557 
                 Q23939 
                 916 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  11 
                 No 
                 Paramyosin 
                 AAK39511 
                 Q967Z0 
                 917 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  13 
                 No 
                 Fatty acid binding protein 
                 AAP35078 
                 Q1M2P5 
                 918 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  14 
                 No 
                 Apolipophorin 
                 BAA04558 
                 Q94507 
                 919 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  15 
                 No 
                 Chitinase 
                 AAD52672 
                 Q9U6R7 
                 920 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  16 
                 No 
                 Gelsolin/villin 
                 AAM64112 
                 Q8MVU3 
                 921 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  17 
                 No 
                 Calcium binding protein 
                 / 
                 / 
                 / 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  18 
                 No 
                 Chitin-binding protein 
                 AAM19082 
                 Q86R84 
                 922 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 / 
                 AIO08850.1 
                 / 
                 923 
               
               
                   farinae  (American 
                 20.0101 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Arginine kinase 
                 ABU97470.1 
                 A7XZJ2 
                 924 
               
               
                   farinae  (American 
                 20.0201 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  21 
                 No 
                 / 
                 AHC94806 
                 B2GM84 
                 925 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  22 
                 No 
                 / 
                 ABG35122 
                 A5X5X4 
                 926 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  24 
                 No 
                 Ubiquinol-cytochrome c 
                 AGI78542 
                 M9RZ95 
                 927 
               
               
                   farinae  (American 
                   
                   
                 reductase binding protein 
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                 homologue 
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Triosephosphate isomerase 
                 AGC56216 
                 L7UZA7 
                 928 
               
               
                   farinae  (American 
                 25.0101 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Triosephosphate isomerase 
                 AIO08860.1 
                 / 
                 929 
               
               
                   farinae  (American 
                 25.0201 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  26 
                 No 
                 Myosin alkali light chain 
                 AIO08852.1 
                 / 
                 930 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  27 
                 No 
                 Serpin 
                 AIO08851.1 
                 / 
                 931 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Heat Shock Protein 
                 AGC56218.1 
                 L7V065 
                 932 
               
               
                   farinae  (American 
                 28.0101 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der f 
                 
                 No 
                 Heat Shock Protein 
                 AIO08848.1 
                 / 
                 933 
               
               
                   farinae  (American 
                 28.0201 
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  29 
                 No 
                 Peptidyl-prolyl cis-trans 
                 AAP35065 
                 A1KXG2 
                 934 
               
               
                   farinae  (American 
                   
                   
                 isomerase (cyclophilin) 
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  30 
                 No 
                 Ferritin 
                 AGC56219.1 
                 L7UZ91 
                 935 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  31 
                 No 
                 Cofilin 
                 AIO08870.1 
                 / 
                 936 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  32 
                 No 
                 Secreted inorganic 
                 AIO08849.1 
                 / 
                 937 
               
               
                   farinae  (American 
                   
                   
                 pyrophosphatase 
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  33 
                 No 
                 alpha-tubulin 
                 AIO08861 
                 / 
                 938 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  34 
                 No 
                 enamine/imine deaminase 
                 / 
                 / 
                 / 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der f  35 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                   farinae  (American 
                   
                   
                   
                   
                   
                   
               
               
                 house dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der m  1 
                 No 
                 Cysteine protease 
                 P16312 
                 P16312 
                 939 
               
               
                   microceras  (House dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 AAB60215 
                 P08176 
                 940 
               
               
                 
                   pteronyssinus 
                 
                 1.0101 
                   
                   
                 (variant) 
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 AAB60215 
                 P08176 
                 941 
               
               
                 
                   pteronyssinus 
                 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 AAB60215 
                 P08176 
                 940 
               
               
                 
                   pteronyssinus 
                 
                 1.0103 
                   
                   
                 (variant) 
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 941 
               
               
                 
                   pteronyssinus 
                 
                 1.0104 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 941 
               
               
                 
                   pteronyssinus 
                 
                 1.0105 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 941 
               
               
                 
                   pteronyssinus 
                 
                 1.0106 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 941 
               
               
                 
                   pteronyssinus 
                 
                 1.0107 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 941 
               
               
                 
                   pteronyssinus 
                 
                 1.0108 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 940 
               
               
                 
                   pteronyssinus 
                 
                 1.0109 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 941 
               
               
                 
                   pteronyssinus 
                 
                 1.0110 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 940 
               
               
                 
                   pteronyssinus 
                 
                 1.0111 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 P08176 
                 940 
               
               
                 
                   pteronyssinus 
                 
                 1.0112 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 ABA39435 
                 Q3HWZ5 
                 942 
               
               
                 
                   pteronyssinus 
                 
                 1.0113 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 942 
               
               
                 
                   pteronyssinus 
                 
                 1.0114 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 943 
               
               
                 
                   pteronyssinus 
                 
                 1.0115 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 942 
               
               
                 
                   pteronyssinus 
                 
                 1.0116 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 943 
               
               
                 
                   pteronyssinus 
                 
                 1.0117 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 942 
               
               
                 
                   pteronyssinus 
                 
                 1.0118 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 943 
               
               
                 
                   pteronyssinus 
                 
                 1.0119 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 943 
               
               
                 
                   pteronyssinus 
                 
                 1.0120 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 943 
               
               
                 
                   pteronyssinus 
                 
                 1.0121 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 943 
               
               
                 
                   pteronyssinus 
                 
                 1.0122 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 / 
                 Q3HWZ5 
                 943 
               
               
                 
                   pteronyssinus 
                 
                 1.0123 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Cysteine protease 
                 CAQ68250 
                 C7T6L6 
                 944 
               
               
                 
                   pteronyssinus 
                 
                 1.0124 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 AAF86462 
                 P49278 
                 945 
               
               
                 
                   pteronyssinus 
                 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 / 
                 P49278 
                 946 
               
               
                 
                   pteronyssinus 
                 
                 2.0102 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 / 
                 P49278 
                 946 
               
               
                 
                   pteronyssinus 
                 
                 2.0103 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 / 
                 P49278 
                 945 
               
               
                 
                   pteronyssinus 
                 
                 2.0104 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 / 
                 P49278 
                 946 
               
               
                 
                   pteronyssinus 
                 
                 2.0105 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 / 
                 P49278 
                 945 
               
               
                 
                   pteronyssinus 
                 
                 2.0106 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 / 
                 P49278 
                 945 
               
               
                 
                   pteronyssinus 
                 
                 2.0107 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 / 
                 P49278 
                 946 
               
               
                 
                   pteronyssinus 
                 
                 2.0108 
                   
                   
                   
                 (variant) 
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 CAQ68249 
                 C7T6L5 
                 947 
               
               
                 
                   pteronyssinus 
                 
                 2.0110 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 CAK22338 
                 Q1H8P8 
                 948 
               
               
                 
                   pteronyssinus 
                 
                 2.0114 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 NPC2 family 
                 CAQ68249 
                 C7T6L5 
                 947 
               
               
                 
                   pteronyssinus 
                 
                 2.0115 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  3 
                 No 
                 Trypsin 
                 AAA19973 
                 P39675 
                 949 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  4 
                 No 
                 Alpha amylase 
                 AAD38942 
                 Q9Y197 
                 950 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 / 
                 CAA35692 
                 P14004 
                 951 
               
               
                 
                   pteronyssinus 
                 
                 5.0101 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 / 
                 AAB32842 
                 P14004 
                 952 
               
               
                 
                   pteronyssinus 
                 
                 5.0102 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  6 
                 No 
                 Chymotrypsin 
                 P49277 
                 P49277 
                 953 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  7 
                 No 
                 Bactericidal permeability- 
                 AAA80264 
                 P49273 
                 954 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                 increasing like protein 
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  8 
                 No 
                 Glutathione S-transferase 
                 AAB32224 
                 P46419 
                 955 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Collagenolytic serine 
                 AAP57077 
                 Q7Z163 
                 956 
               
               
                 
                   pteronyssinus 
                 
                 9.0101 
                   
                 protease 
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Collagenolytic serine 
                 AAN02511 
                 Q8MWR4 
                 957 
               
               
                 
                   pteronyssinus 
                 
                 9.0102 
                   
                 protease 
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  10 
                 No 
                 Tropomyosin 
                 CAA75141 
                 O18416 
                 958 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  11 
                 No 
                 Paramyosin 
                 AAO73464 
                 Q6Y2F9 
                 959 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  13 
                 No 
                 Cytosolic Fatty Acid 
                 ADK92390 
                 E0A8N8 
                 960 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                 Binding Protein 
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  14 
                 No 
                 Apolipophorin 
                 AAM21322 
                 Q8N0N0 
                 961 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Chitinase-like protein 
                 AAY84565 
                 Q4JK69 
                 962 
               
               
                 
                   pteronyssinus 
                 
                 15.0101 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                 
                   Der p 
                 
                 No 
                 Chitinase-like protein 
                 AAY84564 
                 Q4JK70 
                 963 
               
               
                 
                   pteronyssinus 
                 
                 15.0201 
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  18 
                 No 
                 Chitin-binding protein 
                 AAY84563 
                 Q4JK71 
                 964 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  20 
                 No 
                 Arginine kinase 
                 ACD50950 
                 B2ZSY4 
                 965 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  21 
                 No 
                 / 
                 ABC73706 
                 Q2L7C5 
                 966 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  23 
                 No 
                 Peritrophin-like protein 
                 ACB46292 
                 L7N6F8 
                 967 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                 domain (PF01607) 
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dermatophagoides 
                 
                   Der p  24 
                 No 
                 biquinol-cytochrome c 
                 ALA65345.1 
                 A0A0K2GUJ4 
                 968 
               
               
                 
                   pteronyssinus 
                 
                   
                   
                 reductase binding protein 
                   
                   
                   
               
               
                 (European house dust 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dolichovespula 
                 
                   Dol a  5 
                 No 
                 Antigen 5 
                 AAA28303 
                 Q05108 
                 969 
               
               
                   arenaria  (Yellow 
                   
                   
                   
                   
                   
                   
               
               
                 hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dolichovespula 
                 
                 
                   Dol m 
                 
                 No 
                 Phospholipase A1B 
                 CAA47341 
                 Q06478 
                 970 
               
               
                   maculata  (White face 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dolichovespula 
                 
                 
                   Dol m 
                 
                 No 
                 Phospholipase A1B 
                 P53357 
                 P53357 
                 971 
               
               
                   maculata  (White face 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dolichovespula 
                 
                   Dol m  2 
                 No 
                 Hyaluronidase 
                 AAA68279 
                 P49371 
                 972 
               
               
                   maculata  (White face 
                   
                   
                   
                   
                   
                   
               
               
                 hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dolichovespula 
                 
                 
                   Dol m 
                 
                 No 
                 Venom Antigen 5 
                 AA28301 
                 P10736 
                 973 
               
               
                   maculata  (White face 
                 5.0101 
                   
                   
                   
                   
                   
               
               
                 hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dolichovespula 
                 
                 
                   Dol m 
                 
                 No 
                 Venom Antigen 5 
                 AAA28302 
                 P10737 
                 974 
               
               
                   maculata  (White face 
                 5.0102 
                   
                   
                   
                   
                   
               
               
                 hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Eriocheir sinensis 
                 
                   Eri s  2 
                 Yes 
                 ovary development-related 
                 AAO73305 
                 Q5QKR2 
                 975 
               
               
                 ( Eriocheir sinensis ) 
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   Euroglyphus maynei 
                 
                 
                   Eur m 
                 
                 No 
                 Cysteine protease 
                 AAC82351 
                 P25780 
                 976 
               
               
                 (House dust mite) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Euroglyphus maynei 
                 
                 
                   Eur m 
                 
                 No 
                 Cysteine protease 
                 / 
                 P25780 
                 976 
               
               
                 (House dust mite) 
                 1.0102 
                   
                   
                   
                 (variant) 
                   
               
               
                 
                   Euroglyphus maynei 
                 
                 
                   Eur m 
                 
                 No 
                 NPC2 family 
                 AAC8234 
                 Q9TZZ2 
                 977 
               
               
                 (House dust mite) 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Euroglyphus maynei 
                 
                 
                   Eur m 
                 
                 No 
                 NPC2 family 
                 AC82350 
                 Q9TZZ2 
                 977 
               
               
                 (House dust mite) 
                 2.0102 
                   
                   
                   
                 (variant) 
                   
               
               
                 
                   Euroglyphus maynei 
                 
                   Eur m  3 
                 No 
                 Trypsin 
                 AAD10712 
                 O97370 
                 978 
               
               
                 (House dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Euroglyphus maynei 
                 
                   Eur m  4 
                 No 
                 Alpha-amylase 
                 AAD38943 
                 Q9Y196 
                 979 
               
               
                 (House dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Euroglyphus maynei 
                 
                   Eur m  14 
                 No 
                 Apolipophorin 
                 AAF14270 
                 Q9U785 
                 980 
               
               
                 (House dust mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Forcipomyia taiwana 
                 
                   For t  1 
                 No 
                 Serine/threonine-protein 
                 ACD65080 
                 B2ZPG6 
                 981 
               
               
                 (Biting midge) 
                   
                   
                 kinase 
                   
                   
                   
               
               
                 
                   Forcipomyia taiwana 
                 
                   For t  2 
                 No 
                 Eukaryotic translation 
                 ACD65081 
                 B2ZPG7 
                 982 
               
               
                 (Biting midge) 
                   
                   
                 initiation factor 3 subunit 
                   
                   
                   
               
               
                 
                   Glossina morsitans 
                 
                   Glo m  5 
                 No 
                 Tsetse antigen 5, CAP 
                 AAF82096 
                 Q9NBA6 
                 983 
               
               
                 (Savannah Tsetse Fly) 
                   
                   
                 protein superfamily 
                   
                   
                   
               
               
                   
                   
                   
                 member 
                   
                   
                   
               
               
                 
                   Glycyphagus 
                 
                 
                   Gly d 
                 
                 No 
                 Mite group 2 allergen 
                 CAB5997 
                 Q9U5P7 
                 984 
               
               
                   domesticus  (Storage 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Glycyphagus 
                 
                 
                   Gly d 
                 
                 No 
                 Mite group 2 allergen 
                 CAB76459 
                 Q9NFQ4 
                 985 
               
               
                   domesticus  (Storage 
                 2.0201 
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Harmonia axyridis 
                 
                   Har a  1 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Asian ladybeetle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Harmonia axyridis 
                 
                   Har a  2 
                 No 
                 aldehyde dehydrogenase 
                 / 
                 / 
                 / 
               
               
                 (Asian ladybeetle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Homarus americanus 
                 
                 
                   Hom a 
                 
                 Yes 
                 Tropomyosin 
                 AAC48287 
                 O44119-1 
                 986 
               
               
                 (American lobster) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Homarus americanus 
                 
                 
                   Hom a 
                 
                 Yes 
                 Tropomyosin 
                 AAC48288 
                 O44119-2 
                 987 
               
               
                 (American lobster) 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Homarus americanus 
                 
                   Hom a  3 
                 Yes 
                 Myosin light chain 2 
                 / 
                 / 
                 / 
               
               
                 (American lobster) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Homarus americanus 
                 
                   Hom a  6 
                 Yes 
                 Troponin C 
                 P29291 
                 P29291 
                 988 
               
               
                 (American lobster) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                 
                   Lep d 
                 
                 No 
                 / 
                 CAA61419 
                 P80384 
                 989 
               
               
                   destructor  (Storage 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                 
                   Lep d 
                 
                 No 
                 / 
                 CAD32313 
                 P80384 
                 990 
               
               
                   destructor  (Storage 
                 2.0102 
                   
                   
                   
                 (variant) 
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                 
                   Lep d 
                 
                 No 
                 / 
                 CAA58755 
                 P80384 
                 991 
               
               
                   destructor  (Storage 
                 2.0201 
                   
                   
                   
                 (variant) 
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                 
                   Lep d 
                 
                 No 
                 / 
                 CAD32314 
                 P80384 
                 992 
               
               
                   destructor  (Storage 
                 2.0202 
                   
                   
                   
                 (variant) 
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                 
                   Lep d 
                 
                 No 
                 / 
                 CAB62212 
                 Q9U5P2 
                 993 
               
               
                   destructor  (Storage 
                 5.0101 
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                 
                   Lep d 
                 
                 No 
                 / 
                 AAQ73493 
                 Q1M2N1 
                 994 
               
               
                   destructor  (Storage 
                 5.0102 
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                 
                   Lep d 
                 
                 No 
                 / 
                 AAQ73494 
                 Q1M2N0 
                 995 
               
               
                   destructor  (Storage 
                 5.0103 
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                   Lep d  7 
                 No 
                 Bactericidal permeability- 
                 CAB65963 
                 Q9U1G2 
                 996 
               
               
                   destructor  (Storage 
                   
                   
                 increasing like protein 
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                   Lep d  10 
                 No 
                 Tropomyosin 
                 CAB71342 
                 Q9NFZ4 
                 997 
               
               
                   destructor  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepidoglyphus 
                 
                   Lep d  13 
                 No 
                 Fatty acid-binding protein 
                 CAB62213 
                 Q9U5P1 
                 998 
               
               
                   destructor  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lepisma saccharina 
                 
                   Lep s  1 
                 No 
                 Tropomyosin 
                 CAC84590 
                 Q8T380 
                 999 
               
               
                 (Silverfish) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Liposcelis 
                 
                   Lip b  1 
                 No 
                 unknown function 
                 P86712 
                 P86712 
                 1000 
               
               
                 
                   bostrychophila 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (Booklouse) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Litopenaeus vannamei 
                 
                   Lit v  1 
                 Yes 
                 Tropomyosin 
                 ACB38288 
                 B4YAH6 
                 1001 
               
               
                 (White shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Litopenaeus vannamei 
                 
                   Lit v  2 
                 Yes 
                 Arginine kinase 
                 ABI98020 
                 Q004B5 
                 1002 
               
               
                 (White shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Litopenaeus vannamei 
                 
                   Lit v  3 
                 Yes 
                 Myosin, light chain 2 
                 ACC76803 
                 B7SNI3 
                 1003 
               
               
                 (White shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Litopenaeus vannamei 
                 
                   Lit v  4 
                 Yes 
                 Sarcoplasmic calcium- 
                 ACM89179 
                 C7A639 
                 1004 
               
               
                 (White shrimp) 
                   
                   
                 binding protein 
                   
                   
                   
               
               
                 
                   Macrobrachium 
                 
                   Mac r  1 
                 Yes 
                 tropomyosin 
                 ADC55380 
                 D3XNR9 
                 1005 
               
               
                   rosenbergii  (giant 
                   
                   
                   
                   
                   
                   
               
               
                 freshwater prawn) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Melicertus latisulcatus 
                 
                   Mel l  1 
                 Yes 
                 tropomyosin 
                 AGF86397 
                 M4M2H6 
                 1006 
               
               
                 (King Prawn) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Metapenaeus ensis 
                 
                   Met e  1 
                 Yes 
                 tropomyosin 
                 AAA60330 
                 Q25456 
                 1007 
               
               
                 (Shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Myrmecia pilosula 
                 
                   Myr p  1 
                 No 
                 Pilosulin-1 
                 CAA49760 
                 Q07932 
                 1008 
               
               
                 (Australian jumper ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Myrmecia pilosula 
                 
                   Myr p  2 
                 No 
                 pilosulin-3a 
                 AAB36316 
                 Q26464 
                 1009 
               
               
                 (Australian jumper ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Myrmecia pilosula 
                 
                   Myr p  3 
                 No 
                 pilosulin-4.1 
                 BAD36780 
                 Q68Y22 
                 1010 
               
               
                 (Australian jumper ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Pachycondyla chinensis 
                 
                   Pac c  3 
                 No 
                 Antigen 5 
                 ACA96507.1 
                 C0ITL3 
                 1011 
               
               
                 (Asian needle ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Pandalus borealis 
                 
                   Pan b  1 
                 Yes 
                 Tropomyosin 
                 CBY17558 
                 E5BBS3 
                 1012 
               
               
                 (Northern shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Panulirus stimpsoni 
                 
                   Pan s  1 
                 Yes 
                 Tropomyosin 
                 AAC38996 
                 O61379 
                 1013 
               
               
                 (Spiny lobster) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penaeus aztecus 
                 
                   Pen a  1 
                 Yes 
                 Tropomyosin 
                 AAZ76743 
                 Q3Y8M6 
                 1014 
               
               
                 (Brown shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penaeus indicus 
                 
                   Pen i  1 
                 Yes 
                 Tropomyosin 
                 / 
                 / 
                 / 
               
               
                 (Shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penaeus monodon 
                 
                   Pen m  1 
                 Yes 
                 tropomyosin 
                 ADM34184 
                 A1KYZ2 
                 1014 
               
               
                 (Black tiger shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penaeus monodon 
                 
                   Pen m  2 
                 Yes 
                 arginine kinase 
                 AAO15713 
                 Q8I9P7 
                 1015 
               
               
                 (Black tiger shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penaeus monodon 
                 
                   Pen m  3 
                 Yes 
                 Myosin light chain 2 
                 ADM34185 
                 E1A683 
                 1016 
               
               
                 (Black tiger shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penaeus monodon 
                 
                   Pen m  4 
                 Yes 
                 Sarcoplasmic calcium 
                 ADV17343 
                 E7CGC4 
                 1004 
               
               
                 (Black tiger shrimp) 
                   
                   
                 binding protein 
                   
                   
                   
               
               
                 
                   Penaeus monodon 
                 
                   Pen m  6 
                 Yes 
                 Troponin C 
                 ADV17344 
                 E7CGC5 
                 1017 
               
               
                 (Black tiger shrimp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Cr-PII allergen 
                 AAD13533 
                 Q9TZR6 
                 1018 
               
               
                 (American cockroach) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Cr-PII allergen 
                 AAC34312 
                 O18535 
                 1019 
               
               
                 (American cockroach) 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Cr-PII allergen 
                 AAB82404 
                 O18530 
                 1020 
               
               
                 (American cockroach) 
                 1.0103 
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Cr-PII allergen 
                 AAC34737 
                 O18528 
                 1021 
               
               
                 (American cockroach) 
                 1.0104 
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Cr-PII allergen 
                 AAC34736 
                 O18527 
                 1022 
               
               
                 (American cockroach) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                   Per a  2 
                 No 
                 aspartatic protease-like 
                 ADR82198 
                 E7BQV5 
                 1023 
               
               
                 (American cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Arylphorins/TO 
                 AAB09629 
                 Q25641 
                 1024 
               
               
                 (American cockroach) 
                 3.0101 
                   
                 Arthropod hemocyanins 
                   
                   
                   
               
               
                   
                   
                   
                 (Cr-PI Allergen) 
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Arylphorins/TO 
                 AAB09632 
                 Q94643 
                 1025 
               
               
                 (American cockroach) 
                 3.0201 
                   
                 Arthropod hemocyanins 
                   
                   
                   
               
               
                   
                   
                   
                 (Cr-PI Allergen) 
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Arylphorins/TO 
                 AAB62731 
                 Q25640 
                 1026 
               
               
                 (American cockroach) 
                 3.0202 
                   
                 Arthropod hemocyanins 
                   
                   
                   
               
               
                   
                   
                   
                 (Cr-PI Allergen) 
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                 
                   Per a 
                 
                 No 
                 Arylphorins/TO 
                 AAB63595 
                 Q25639 
                 1027 
               
               
                 (American cockroach) 
                 3.0203 
                   
                 Arthropod hemocyanins 
                   
                   
                   
               
               
                   
                   
                   
                 (Cr-PI Allergen) 
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                   Per a  6 
                 No 
                 Troponin C 
                 AAX33730 
                 Q1M0Y3 
                 1028 
               
               
                 (American cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                   Per a  7 
                 No 
                 Tropomyosin 
                 CAB38086 
                 Q9UB83 
                 1029 
               
               
                 (American cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                   Per a  9 
                 No 
                 Arginine kinase 
                 ACA00204 
                 / 
                 1030 
               
               
                 (American cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                   Per a  10 
                 No 
                 Serine protease 
                 AAX33734 
                 Q1M0X9 
                 1031 
               
               
                 (American cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                   Per a  11 
                 No 
                 alpha amylase 
                 AKH04310 
                 / 
                 1032 
               
               
                 (American cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Periplaneta americana 
                 
                   Per a  12 
                 No 
                 Chitinase 
                 AKH04311 
                 / 
                 1033 
               
               
                 (American cockroach) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Plodia interpunctella 
                 
                   Plo i  1 
                 No 
                 Arginine kinase 
                 CAC85911 
                 Q95PM9 
                 1034 
               
               
                 (Indianmeal moth) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Plodia interpunctella 
                 
                   Plo i  2 
                 No 
                 Thioredoxin 
                 CBW45298 
                 E1XUQ3 
                 1035 
               
               
                 (Indianmeal moth) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes annularis 
                 
                   Pol a  1 
                 No 
                 Phospholipase A1B 
                 AD52615 
                 Q9U6W0 
                 1036 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes annularis 
                 
                   Pol a  2 
                 No 
                 Hyaluronidase 
                 AAD52616 
                 Q9U6V9 
                 1037 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes annularis 
                 
                   Pol a  5 
                 No 
                 Antigen 5 
                 AAA29793 
                 Q05109 
                 1038 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes dominulus 
                 
                 
                   Pol d 
                 
                 No 
                 Phospholipase A1-1 
                 AAS67041 
                 Q6Q252 
                 1039 
               
               
                 (Mediterranean paper 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes dominulus 
                 
                 
                   Pol d 
                 
                 No 
                 Phospholipase A1-2 
                 AAS67042 
                 Q6Q251 
                 1040 
               
               
                 (Mediterranean paper 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes dominulus 
                 
                 
                   Pol d 
                 
                 No 
                 Phospholipase A1-3 
                 AAS67043 
                 Q6Q250 
                 1041 
               
               
                 (Mediterranean paper 
                 1.0103 
                   
                   
                   
                   
                   
               
               
                 wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes dominulus 
                 
                 
                   Pol d 
                 
                 No 
                 Phospholipase A1-4 
                 AAS67044 
                 Q6Q249 
                 1042 
               
               
                 (Mediterranean paper 
                 1.0104 
                   
                   
                   
                   
                   
               
               
                 wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes dominulus 
                 
                   Pol d  4 
                 No 
                 Serine protease 
                 AAP37412 
                 Q7Z269 
                 1043 
               
               
                 (Mediterranean paper 
                   
                   
                   
                   
                   
                   
               
               
                 wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes dominulus 
                 
                   Pol d  5 
                 No 
                 Antigen 5 
                 AAT95010 
                 Q68KJ8 
                 1044 
               
               
                 (Mediterranean paper 
                   
                   
                   
                   
                   
                   
               
               
                 wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes exclamans 
                 
                   Pol e  1 
                 No 
                 Phospholipase A1 
                 / 
                 / 
                 / 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes exclamans 
                 
                   Pol e  4 
                 No 
                 Serine protease 
                 / 
                 / 
                 / 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes exclamans 
                 
                   Pol e  5 
                 No 
                 Antigen 5 
                 AAT95009 
                 Q68KJ9 
                 1045 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Polistes fuscatus 
                 
                   Pol f  5 
                 No 
                 Antigen 5 
                 P35780 
                 P35780 
                 1046 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                   Polistes gallicus  (Wasp) 
                   Pol g  1 
                 No 
                 Phospholipase A1 
                 P83542 
                 P83542 
                 1047 
               
               
                   Polistes gallicus  (Wasp) 
                   Pol g  5 
                 No 
                 Antigen 5 
                 P83377 
                 P83377 
                 1048 
               
               
                 
                   Polistes metricus 
                 
                   Pol m  5 
                 No 
                 Antigen 5 
                 P35780 
                 P35780 
                 1046 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                   Polybia paulista  (Wasp) 
                   Poly p  1 
                 No 
                 Phospholipase A1 
                 ABN13879 
                 A2VBC4 
                 1049 
               
               
                   Polybia paulista  (Wasp) 
                   Poly p  2 
                 No 
                 Hyaluronidase 
                 / 
                 P86687 
                 1050 
               
               
                   Polybia paulista  (Wasp) 
                 
                   Poly p 
                 
                 No 
                 Venom group 5 
                 / 
                 C0HJV9 
                 / 
               
               
                   
                 5.0101 
                   
                   
                   
                   
                   
               
               
                   Polybia paulista  (Wasp) 
                 
                   Poly p 
                 
                 No 
                 Venom group 5 
                 / 
                 P86686 
                 1051 
               
               
                   
                 5.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Polybia scutellaris 
                 
                   Poly s  5 
                 No 
                   
                 / 
                 / 
                 / 
               
               
                 (Wasp) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   tastacus leptodactylus 
                 
                   Pon l  4 
                 Yes 
                 Sarcoplasmic calcium- 
                 P05946 
                 P05946 
                 1052 
               
               
                 (Narrow-clawed 
                   
                   
                 binding protein 
                   
                   
                   
               
               
                 crayfish) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   tastacus leptodactylus 
                 
                   Pon l  7 
                 Yes 
                 Troponin I 
                 P05547 
                 P05547 
                 1053 
               
               
                 (Narrow-clawed 
                   
                   
                   
                   
                   
                   
               
               
                 crayfish) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Portunus pelagicus 
                 
                   Por p  1 
                 Yes 
                 Tropomyosin 
                 AGE44125 
                 M1H607 
                 1054 
               
               
                 (Blue swimmer crab) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solenopsis geminata 
                 
                   Sol g  2 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Tropical fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solenopsis geminata 
                 
                   Sol g  3 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Tropical fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solenopsis geminata 
                 
                   Sol g  4 
                 No 
                 / 
                 AAF65312 
                 Q9NH75 
                 1055 
               
               
                 (Tropical fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                   Solenopsis invicta  (Red 
                   Sol i  1 
                 No 
                 Phospholipase A1B 
                 AAT95008 
                 Q68KK0 
                 1056 
               
               
                 imported fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                   Solenopsis invicta  (Red 
                   Sol i  2 
                 No 
                 / 
                 P35775 
                 P35775 
                 1057 
               
               
                 imported fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                   Solenopsis invicta  (Red 
                   Sol i  3 
                 No 
                 / 
                 AAB65434 
                 P35778 
                 1058 
               
               
                 imported fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                   Solenopsis invicta  (Red 
                   Sol i  4 
                 No 
                 / 
                 AAC97369 
                 P35777 
                 1059 
               
               
                 imported fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solenopsis richteri 
                 
                   Sol r  2 
                 No 
                 / 
                 P35776 
                 P35776 
                 1060 
               
               
                 (Black fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solenopsis richteri 
                 
                   Sol r  3 
                 No 
                 / 
                 P35779 
                 P35779 
                 1061 
               
               
                 (Black fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solenopsis saevissima 
                 
                   Sol s  2 
                 No 
                 / 
                 ABC58726 
                 A5X2H7 
                 1062 
               
               
                 (Brazilian fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solenopsis saevissima 
                 
                   Sol s  3 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Brazilian fire ant) 
                   
                   
                   
                   
                   
                   
               
               
                   Tabanus yao  (Horsefly) 
                   Tab y  1 
                 No 
                 Apyrase 
                 ADX78255 
                 F1JZ10 
                 1063 
               
               
                   Tabanus yao  (Horsefly) 
                   Tab y  2 
                 No 
                 Hyaluronidase 
                 ADM18346 
                 E0XKJ9 
                 1064 
               
               
                   Tabanus yao  (Horsefly) 
                   Tab y  5 
                 No 
                 Antigen 5-related protein, 
                 ADM18345 
                 E0XKJ8 
                 1065 
               
               
                   
                   
                   
                 CAP protein superfamily 
                   
                   
                   
               
               
                   
                   
                   
                 member 
                   
                   
                   
               
               
                 
                   Thaumetopoea 
                 
                   Tha p  1 
                 No 
                 / 
                 Q7M4K8 
                 Q7M4K8 
                 1066 
               
               
                   pityocampa  (Pine 
                   
                   
                   
                   
                   
                   
               
               
                 processionary moth) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Thaumetopoea 
                 
                   Tha p  2 
                 No 
                 / 
                 P86360 
                 P86360 
                 1067 
               
               
                   pityocampa  (Pine 
                   
                   
                   
                   
                   
                   
               
               
                 processionary moth) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triatoma protracta 
                 
                   Tria p  1 
                 No 
                 Procalin 
                 AAF07903 
                 Q9U6R6 
                 1068 
               
               
                 (California kissing bug) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Tyrophagus 
                 
                   Tyr p  2 
                 No 
                 NPC2 family 
                 CAA73221 
                 O02380 
                 1069 
               
               
                   putrescentiae  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Tyrophagus 
                 
                   Tyr p  3 
                 No 
                 Trypsin 
                 ABZ81991 
                 C6ZDB5 
                 1070 
               
               
                   putrescentiae  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Tyrophagus 
                 
                   Tyr p  10 
                 No 
                 Tropomyosin 
                 AAT40866 
                 Q6IUP9 
                 1071 
               
               
                   putrescentiae  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Tyrophagus 
                 
                   Tyr p  13 
                 No 
                 Fatty-acid binding protein 
                 AAU11502 
                 Q66RP5 
                 1072 
               
               
                   putrescentiae  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Tyrophagus 
                 
                   Tyr p  28 
                 No 
                 Heat shock protein 
                 AOD75395.1 
                 / 
                 1073 
               
               
                   putrescentiae  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Tyrophagus 
                 
                   Tyr p  34 
                 No 
                 Troponin C 
                 ACL36923 
                 D2DGW3 
                 1074 
               
               
                   putrescentiae  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Tyrophagus 
                 
                   Tyr p  35 
                 No 
                 Aldehyde dehydrogenase 
                 AOD75396.1 
                 / 
                 1075 
               
               
                   putrescentiae  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Tyrophagus 
                 
                   Tyr p  36 
                 No 
                 Profilin 
                 AOD75399.1 
                 / 
                 1076 
               
               
                   putrescentiae  (Storage 
                   
                   
                   
                   
                   
                   
               
               
                 mite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespa crabro 
                 
                   Vesp c  1 
                 No 
                 Phospholipase A1B 
                 P0CH87 
                 P0CH87 
                 1077 
               
               
                 (European hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespa crabro 
                 
                 
                   Vesp c 
                 
                 No 
                 Antigen 5 
                 P35781 
                 P35781 
                 1078 
               
               
                 (European hornet) 
                 5.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Vespa crabro 
                 
                 
                   Vesp c 
                 
                 No 
                 Antigen 5 
                 P35782 
                 P35782 
                 1079 
               
               
                 (European hornet) 
                 5.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Vespa magnifica 
                 
                 
                   Vesp ma 
                 
                 No 
                 Hyaluronidase 
                 / 
                 / 
                 / 
               
               
                 (Hornet) 
                 2 
                   
                   
                   
                   
                   
               
               
                 
                   Vespa magnifica 
                 
                 
                   Vesp ma 
                 
                 No 
                 Antigen 5, member of PR- 
                 / 
                 / 
                 / 
               
               
                 (Hornet) 
                 5 
                   
                 1 family 
                   
                   
                   
               
               
                 
                   Vespa mandarinia 
                 
                   Vesp m  1 
                 No 
                 Phospholipase A1B 
                 / 
                 / 
                 / 
               
               
                 (Giant asian hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespa mandarinia 
                 
                   Vesp m  5 
                 No 
                 Antigen 5 
                 P81657 
                 P81657 
                 1080 
               
               
                 (Giant asian hornet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula flavopilosa 
                 
                   Ves f  5 
                 No 
                 Antigen 5 
                 P35783 
                 P35783 
                 1081 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula germanica 
                 
                   Ves g  5 
                 No 
                 Antigen 5 
                 CAJ28930 
                 P35784 
                 1082 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula maculifrons 
                 
                   Ves m  1 
                 No 
                 Phospholipase A1B 
                 P51528 
                 P51528 
                 1083 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula maculifrons 
                 
                   Ves m  2 
                 No 
                 Hyaluronidase 
                 P0CH89 
                 P0CH89 
                 1084 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula maculifrons 
                 
                   Ves m  5 
                 No 
                 Antigen 5 
                 P35760 
                 P35760 
                 1085 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula pensylvanica 
                 
                   Ves p  5 
                 No 
                 Antigen 5 
                 P35785 
                 P35785 
                 1086 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula squamosa 
                 
                   Ves s  1 
                 No 
                 Phospholipase A1B 
                 P0CH86 
                 P0CH86 
                 1087 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula squamosa 
                 
                   Ves s  5 
                 No 
                 Antigen 5 
                 P35786 
                 P35786 
                 1088 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                   Vespula vidua  (Wasp) 
                   Ves vi  5 
                 No 
                 Antigen 5 
                 P35787 
                 P35787 
                 1089 
               
               
                 
                   Vespula vulgaris 
                 
                   Ves v  1 
                 No 
                 Phospholipase A1B 
                 AAB48072 
                 P49369 
                 1090 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula vulgaris 
                 
                 
                   Ves v 
                 
                 No 
                 Hyaluronidase 
                 CAI77218 
                 P49370 
                 1091 
               
               
                 (Yellow jacket) 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Vespula vulgaris 
                 
                 
                   Ves v 
                 
                 No 
                 Hyaluronidase 
                 AAX14718 
                 Q5D7H4 
                 1092 
               
               
                 (Yellow jacket) 
                 2.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Vespula vulgaris 
                 
                   Ves v  3 
                 No 
                 Dipeptidylpeptidase IV 
                 ACA00159 
                 B1A4F7 
                 1093 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula vulgaris 
                 
                   Ves v  5 
                 No 
                 Antigen 5 
                 AAA30333 
                 Q05110 
                 1094 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Vespula vulgaris 
                 
                   Ves v  6 
                 No 
                 Vitellogenin 
                 AER70365 
                 G8IIT0 
                 1095 
               
               
                 (Yellow jacket) 
                   
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Animalia Chordata 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 
                   Bos domesticus Bos 
                 
                 
                   Bos d 
                 
                 No 
                 Lipocalin 
                 AAB08720 
                 Q28133 
                 1096 
               
               
                   taurus  (domestic cattle) 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                 
                   Bos d 
                 
                 No 
                 Lipocalin 
                 NP_777186 
                 Q28133 
                 1097 
               
               
                   taurus  (domestic cattle) 
                 2.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  3 
                 No 
                 S100 calcium-binding 
                 AAA91101 
                 Q28050 
                 1098 
               
               
                   taurus  (domestic cattle) 
                   
                   
                 protein A7 
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  4 
                 Yes 
                 Alpha-lactalbumin 
                 AAA30615 
                 P00711 
                 1099 
               
               
                   taurus  (domestic cattle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  5 
                 Yes 
                 Beta-lactoglobulin 
                 CAA32835 
                 P02754 
                 1100 
               
               
                   taurus  (domestic cattle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  6 
                 Yes 
                 Serum albumin 
                 AAA51411 
                 P02769 
                 1101 
               
               
                   taurus  (domestic cattle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  7 
                 Yes 
                 Immunoglobulin 
                 / 
                 / 
                 / 
               
               
                   taurus  (domestic cattle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  8 
                 Yes 
                 Caseins (see individual 
                 / 
                 / 
                 / 
               
               
                   taurus  (domestic cattle) 
                   
                   
                 casein 9-12) 
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  9 
                 Yes 
                 alphaS1-casein 
                 NP_851372 
                 P02662 
                 1102 
               
               
                   taurus  (domestic cattle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  10 
                 Yes 
                 alphaS2-casein 
                 NP_776953 
                 P02663 
                 1103 
               
               
                   taurus  (domestic cattle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  11 
                 Yes 
                 beta-casein 
                 XP_005902099 
                 P02666 
                 1104 
               
               
                   taurus  (domestic cattle) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bos domesticus Bos 
                 
                   Bos d  12 
                 Yes 
                 kappa-casein 
                 NP_776719 
                 P02668 
                 1105 
               
               
                   taurus  (domestic cattle) 
                   
                   
                   
                   
                   
                   
               
               
                   Canis familiaris  (dog) 
                   Can f  1 
                 No 
                 Lipocalin 
                 AAC48794 
                 O18873 
                 1106 
               
               
                   Canis familiaris  (dog) 
                   Can f  2 
                 No 
                 Lipocalin 
                 AAC48795 
                 O18874 
                 1107 
               
               
                   Canis familiaris  (dog) 
                   Can f  3 
                 No 
                 Serum albumin 
                 BAC10663 
                 P49822 
                 1108 
               
               
                   Canis familiaris  (dog) 
                   Can f  4 
                 No 
                 Lipocalin 
                 ACY38525 
                 D7PBH4 
                 1109 
               
               
                   Canis familiaris  (dog) 
                   Can f  5 
                 No 
                 Arginine esterase, prostatic 
                 CAA68720 
                 P09582 
                 1110 
               
               
                   
                   
                   
                 kallikrein 
                   
                   
                   
               
               
                   Canis familiaris  (dog) 
                   Can f  6 
                 No 
                 Lipocalin 
                 CCF72371 
                 H2B3G5 
                 1111 
               
               
                   Canis familiaris  (dog) 
                   Can f  7 
                 No 
                 Epididymal Secretory 
                 AAB34263.1 
                 Q28895 
                 1112 
               
               
                   
                   
                   
                 Protein E1, or Niemann 
                   
                   
                   
               
               
                   
                   
                   
                 Pick type C2 protein 
                   
                   
                   
               
               
                   Cavia porcellus  (guinea 
                   Cav p  1 
                 No 
                 Lipocalin 
                 P83507 
                 P83507 
                 1113 
               
               
                 pig) 
                   
                   
                   
                   
                   
                   
               
               
                   Cavia porcellus  (guinea 
                   Cav p  2 
                 No 
                 Lipocalin 
                 CAX62129 
                 F0UZ11 
                 1114 
               
               
                 pig) 
                   
                   
                   
                   
                   
                   
               
               
                   Cavia porcellus  (guinea 
                   Cav p  3 
                 No 
                 Lipocalin 
                 CAX62130 
                 F0UZ12 
                 1115 
               
               
                 pig) 
                   
                   
                   
                   
                   
                   
               
               
                   Cavia porcellus  (guinea 
                   Cav p  4 
                 No 
                 Serum albumin 
                 AAQ20088 
                 Q6WDN9 
                 1116 
               
               
                 pig) 
                   
                   
                   
                   
                   
                   
               
               
                   Cavia porcellus  (guinea 
                   Cav p  6 
                 No 
                 Lipocalin 
                 CAX62131 
                 S0BDX9 
                 1117 
               
               
                 pig) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Clupea harengus 
                 
                 
                   Clu h 
                 
                 Yes 
                 Beta-parvalbumin 
                 CAQ72970 
                 C6GKU6 
                 1118 
               
               
                 (Atlantic herring) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Clupea harengus 
                 
                 
                   Clu h 
                 
                 Yes 
                 Beta-parvalbumin 
                 CAQ72971 
                 C6GKU7 
                 1119 
               
               
                 (Atlantic herring) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Clupea harengus 
                 
                 
                   Clu h 
                 
                 Yes 
                 Beta-parvalbumin 
                 CAQ72972 
                 C6GKU8 
                 1120 
               
               
                 (Atlantic herring) 
                 1.0301 
                   
                   
                   
                   
                   
               
               
                 
                   Cyprinus carpio 
                 
                 
                   Cyp c 
                 
                 Yes 
                 beta-parvalbumin 
                 CAC83658 
                 Q8UUS3 
                 1121 
               
               
                 (Common carp) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Cyprinus carpio 
                 
                 
                   Cyp c 
                 
                 Yes 
                 beta-parvalbumin 
                 CAC83659 
                 Q8UUS2 
                 1122 
               
               
                 (Common carp) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Equus caballus 
                 
                   Equ c  1 
                 No 
                 Lipocalin 
                 AAC48691 
                 Q95182 
                 1123 
               
               
                 (domestic horse) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Equus caballus 
                 
                 
                   Equ c 
                 
                 No 
                 Lipocalin 
                 P81216 
                 P81216 
                 1124 
               
               
                 (domestic horse) 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Equus caballus 
                 
                 
                   Equ c 
                 
                 No 
                 Lipocalin 
                 P81217 
                 P81217 
                 1125 
               
               
                 (domestic horse) 
                 2.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Equus caballus 
                 
                   Equ c  3 
                 No 
                 Serum albumin 
                 CAA52194 
                 P35747 
                 1126 
               
               
                 (domestic horse) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Equus caballus 
                 
                   Equ c  4 
                 No 
                 Latherin 
                 AAM09530 
                 P82615 
                 1127 
               
               
                 (domestic horse) 
                   
                   
                   
                   
                   
                   
               
               
                   Felis domesticus  (cat) 
                   Fel d  1 
                 No 
                 Uteroglobin (chain 1) 
                 AAC37318 
                 P30438 
                 1128 
               
               
                   
                   
                   
                   
                 (chain 1) 
                 (chain 1) 
                   
               
               
                   Felis domesticus  (cat) 
                   Fel d  1 
                 No 
                 Uteroglobin (chain 2) 
                 AAC41616 
                 P30440 
                 1129 
               
               
                   
                   
                   
                   
                 (chain 2) 
                 (chain 2) 
                   
               
               
                   Felis domesticus  (cat) 
                   Fel d  2 
                 No 
                 Serum albumin 
                 CAA59279 
                 P49064 
                 1130 
               
               
                   Felis domesticus  (cat) 
                   Fel d  3 
                 No 
                 Cystatin 
                 AAL49391 
                 Q8WNR9 
                 1131 
               
               
                   Felis domesticus  (cat) 
                   Fel d  4 
                 No 
                 Lipocalin 
                 AAS77253 
                 Q5VFH6 
                 1132 
               
               
                   Felis domesticus  (cat) 
                   Fel d  5w 
                 No 
                 Immunoglobulin A 
                 / 
                 / 
                 / 
               
               
                   Felis domesticus  (cat) 
                   Fel d  6w 
                 No 
                 Immunoglobulin M 
                 / 
                 / 
                 / 
               
               
                   Felis domesticus  (cat) 
                   Fel d  7 
                 No 
                 von Ebner gland protein 
                 ADK56160 
                 E5D2Z5 
                 1133 
               
               
                   Felis domesticus  (cat) 
                   Fel d  8 
                 No 
                 Latherin-like protein 
                 ADM15668 
                 F6K0R4 
                 1134 
               
               
                   Gadus callarias  (Baltic 
                   Gad c  1 
                 Yes 
                 Beta-parvalbumin 
                 P02622 
                 P02622 
                 1135 
               
               
                 cod) 
                   
                   
                   
                   
                   
                   
               
               
                   Gadus morhua  (Atlantic 
                 
                   Gad m 
                 
                 Yes 
                 Beta-parvalbumin 
                 AAK63086 
                 Q90YL0 
                 1136 
               
               
                 cod) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                   Gadus morhua  (Atlantic 
                 
                   Gad m 
                 
                 Yes 
                 Beta-parvalbumin 
                 CAM56785 
                 A5I873 
                 1137 
               
               
                 cod) 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                   Gadus morhua  (Atlantic 
                 
                   Gad m 
                 
                 Yes 
                 Beta-parvalbumin 
                 AAK63087 
                 Q90YK9 
                 1138 
               
               
                 cod) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                   Gadus morhua  (Atlantic 
                 
                   Gad m 
                 
                 Yes 
                 Beta-parvalbumin 
                 CAM56786 
                 A5I874 
                 1139 
               
               
                 cod) 
                 1.0202 
                   
                   
                   
                   
                   
               
               
                   Gadus morhua  (Atlantic 
                   Gad m  2 
                 Yes 
                 Beta-enolase 
                 B3A0L6 
                 B3A0L6 
                 1140 
               
               
                 cod) 
                   
                   
                   
                   
                   
                   
               
               
                   Gadus morhua  (Atlantic 
                   Gad m  3 
                 Yes 
                 Fructose-bisphosphate 
                 P86980 
                 P86980 
                 1141 
               
               
                 cod) 
                   
                   
                 aldolase A 
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  1 
                 Yes 
                 Ovomucoid 
                 P01005 
                 P01005 
                 1142 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  2 
                 Yes 
                 Ovalbumin 
                 CAA23682 
                 P01012 
                 1143 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  3 
                 Yes 
                 Ovotransferrin 
                 CAA26040 
                 P02789 
                 1144 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  4 
                 Yes 
                 Lysozyme C 
                 CAA23711 
                 P00698 
                 1145 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  5 
                 Yes 
                 Serum albumin 
                 CAA43098 
                 P19121 
                 1146 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  6 
                 Yes 
                 YGP42 
                 BAA13973 
                 P87498 
                 1147 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  7 
                 Yes 
                 Myosin light chain 1f 
                 K02610.1 
                 P02604 
                 1148 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  8 
                 Yes 
                 alpha-parvalbumin 
                 CAX32963 
                 C1L370 
                 1149 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  9 
                 Yes 
                 Beta-enolase 
                 NP_990450 
                 P07322 
                 1150 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Gallus domesticus 
                 
                   Gal d  10 
                 Yes 
                 Aldolase 
                 / 
                 / 
                 / 
               
               
                 (chicken) 
                   
                   
                   
                   
                   
                   
               
               
                   Homo sapiens  (human 
                   Hom s  1 
                 No 
                 Squamous cell carcinoma 
                 BAA24056 
                 O43290 
                 1151 
               
               
                 autoallergens) 
                   
                   
                 antigen SART-1 
                   
                   
                   
               
               
                   Homo sapiens  (human 
                   Hom s  2 
                 No 
                 Nascent polypeptide- 
                 AAK57544 
                 Q13765 
                 1152 
               
               
                 autoallergens) 
                   
                   
                 associated complex alpha 
                   
                   
                   
               
               
                   
                   
                   
                 subunit 
                   
                   
                   
               
               
                   Homo sapiens  (human 
                   Hom s  3 
                 No 
                 BCD7B protein 
                 CAA62012 
                 Q13845 
                 1153 
               
               
                 autoallergens) 
                   
                   
                   
                   
                   
                   
               
               
                   Homo sapiens  (human 
                   Hom s  4 
                 No 
                 Atopy related autoantigen 
                 CAA76830 
                 O75785 
                 1154 
               
               
                 autoallergens) 
                   
                   
                 CALC 
                   
                   
                   
               
               
                   Homo sapiens  (human 
                   Hom s  5 
                 No 
                 Keratin, type II 
                 AAH69269 
                 P02538 
                 1155 
               
               
                 autoallergens) 
                   
                   
                 cytoskeletal 6A 
                   
                   
                   
               
               
                 
                   Lates calcarifer 
                 
                 
                   Lat c 
                 
                 Yes 
                 Beta 1-parvalbumin 
                 AHW83198 
                 Q5IRB2 
                 1156 
               
               
                 (Barramundi) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Lates calcarifer 
                 
                 
                   Lat c 
                 
                 Yes 
                 Beta 2-parvalbumin 
                 AAT45383 
                 Q6ITU9 
                 1157 
               
               
                 (Barramundi) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Lepidorhombus 
                 
                   Lep w  1 
                 Yes 
                 Beta-parvalbumin 
                 CAP17694 
                 B5WX08 
                 1158 
               
               
                   whiffiagonis  (Megrim, 
                   
                   
                   
                   
                   
                   
               
               
                 Whiff, Turbot fish) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Mesocricetus auratus 
                 
                   Mes a  1 
                 No 
                 lipocalin 
                 AAD55792 
                 Q9QXU1 
                 1159 
               
               
                 (Golden hamster, 
                   
                   
                   
                   
                   
                   
               
               
                 Syrian hamster) 
                   
                   
                   
                   
                   
                   
               
               
                   Mus musculus  (mouse) 
                 
                   Mus m 
                 
                 No 
                 Lipocalin/urinary 
                 CAA26953 
                 P02762 
                 1160 
               
               
                   
                 1.0101 
                   
                 prealbumin 6 
                   
                   
                   
               
               
                   Mus musculus  (mouse) 
                 
                   Mus m 
                 
                 No 
                 Lipocalin/urinary 
                 AAA39768 
                 P11589 
                 1161 
               
               
                   
                 1.0102 
                   
                 prealbumin 2 
                   
                   
                   
               
               
                 
                   Oncorhynchus keta 
                 
                   Onc k  5 
                 Yes 
                 beta-prime-component of 
                 BAJ07603 
                 D5MU14 
                 1162 
               
               
                 (Chum salmon) 
                   
                   
                 vitellogenin 
                   
                   
                   
               
               
                 
                   Oncorhynchus mykiss 
                 
                 
                   Onc m 
                 
                 Yes 
                 Beta1-parvalbumin 
                 P86431 
                 P86431 
                 1163 
               
               
                 (Rainbow trout) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Oncorhynchus mykiss 
                 
                 
                   Onc m 
                 
                 Yes 
                 Beta2-parvalbumin 
                 P86432 
                 P86432 
                 1164 
               
               
                 (Rainbow trout) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Oreochromis 
                 
                   Ore m  4 
                 Yes 
                 Tropomyosin 
                 AFV53352 
                 K4PEK4 
                 1165 
               
               
                 
                   mossambicus 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (Mozambique tilapia) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Oryctolagus cuniculus 
                 
                   Ory c  1 
                 No 
                 Lipocalin 
                 / 
                 / 
                 / 
               
               
                 (rabbit) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Oryctolagus cuniculus 
                 
                 
                   Ory c 
                 
                 No 
                 Lipophilin CL2 
                 AAG42806 
                 Q9GK63 
                 1166 
               
               
                 (rabbit) 
                 3.A.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Oryctolagus cuniculus 
                 
                 
                   Ory c 
                 
                 No 
                 Lipophilin AL 
                 AAG42802 
                 Q9GK67 
                 1167 
               
               
                 (rabbit) 
                 3.B.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Oryctolagus cuniculus 
                 
                   Ory c  4 
                 No 
                 Lipocalin 
                 CCC15303 
                 U6C8D6 
                 1168 
               
               
                 (rabbit) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phodopus sungorus 
                 
                   Phod s  1 
                 No 
                 Lipocalin 
                 AGT28425 
                 S5ZYD3 
                 1169 
               
               
                 (Siberian hamster) 
                   
                   
                   
                   
                   
                   
               
               
                   Rana esculenta  (edible 
                   Ran e  1 
                 Yes 
                 Alpha-parvalbumin 
                 CAC83046 
                 Q8JIU2 
                 1170 
               
               
                 frog) 
                   
                   
                   
                   
                   
                   
               
               
                   Rana esculenta  (edible 
                   Ran e  2 
                 Yes 
                 Beta-parvalbumin 
                 CAC95152 
                 Q8JIU1 
                 1171 
               
               
                 frog) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Rastrelliger kanagurta 
                 
                   Ras k  1 
                 Yes 
                 Parvalbumin 
                 ANW10058 
                 / 
                 1172 
               
               
                 (Indian Mackerel) 
                   
                   
                   
                   
                   
                   
               
               
                   Rattus norvegicus  (Rat) 
                   Rat n  1 
                 No 
                 Alpha-2u-globulin/ 
                 AAA41198 
                 P02761 
                 1173 
               
               
                   
                   
                   
                 Lipocalin 
                   
                   
                   
               
               
                   Salmo salar  (Atlantic 
                   Sal s  1 
                 Yes 
                 Beta1-parvalbumin 
                 CAA66403 
                 Q91482 
                 1174 
               
               
                 salmon) 
                   
                   
                   
                   
                   
                   
               
               
                   Salmo salar  (Atlantic 
                   Sal s  2 
                 Yes 
                 Beta-Enolase 
                 ACH70932 
                 B5DGQ7 
                 1175 
               
               
                 salmon) 
                   
                   
                   
                   
                   
                   
               
               
                   Salmo salar  (Atlantic 
                   Sal s  3 
                 Yes 
                 Aldolase A 
                 ACH70901 
                 B5DGM7 
                 1176 
               
               
                 salmon) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sardinops sagax 
                 
                   Sar sa  1 
                 Yes 
                 Beta-parvalbumin 
                 CAQ68366 
                 B3WFF7 
                 1177 
               
               
                 (Pacific pilchard) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sebastes marinus 
                 
                 
                   Seb m 
                 
                 Yes 
                 Beta-parvalbumin 
                 CAQ72968 
                 C6GKU4 
                 1178 
               
               
                 (Ocean perch, redfish, 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 snapper) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sebastes marinus 
                 
                 
                   Seb m 
                 
                 Yes 
                 Beta-parvalbumin 
                 CAQ72969 
                 C6GKU5 
                 1179 
               
               
                 (Ocean perch, redfish, 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 snapper) 
                   
                   
                   
                   
                   
                   
               
               
                   Sus scrofa  (Domestic 
                   Sus s  1 
                 Yes 
                 Serum albumin 
                 AAA30988.1 
                 P08835 
                 1180 
               
               
                 pig) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Thunnus albacares 
                 
                   Thu a  1 
                 Yes 
                 Beta-parvalbumin 
                 CAQ72967 
                 C6GKU3 
                 1181 
               
               
                 (Yellowfin tuna) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Thunnus albacares 
                 
                   Thu a  2 
                 Yes 
                 Beta-enolase 
                 P86978 
                 P86978 
                 1182 
               
               
                 (Yellowfin tuna) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Thunnus albacares 
                 
                   Thu a  3 
                 Yes 
                 Aldolase A 
                 P86979 
                 P86979 
                 1183 
               
               
                 (Yellowfin tuna) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Xiphias gladius 
                 
                   Xip g  1 
                 Yes 
                 Beta-parvalbumin 
                 CAR48256 
                 B9W4C2 
                 1184 
               
               
                 (Swordfish) 
                   
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Animalia Cnidaria and Mollusca 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   Dendronephthya  sp. 
                   Den n  1 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Soft Coral) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Crassostrea 
                 
                 
                   Cra g 
                 
                 Yes 
                 tropomyosin 
                 ARX70262.1 
                 / 
                 1185 
               
               
                   gigas  (Pacific Ovster) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Crassostrea 
                 
                 
                   Cra g 
                 
                 Yes 
                 tropomyosin 
                 BAH10152.1 
                 / 
                 1186 
               
               
                   gigas  (Pacific Oyster) 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                   Haliotis laevigata  × 
                   Hal l  1 
                 Yes 
                 Tropomyosin 
                 APG42675 
                 / 
                 1187 
               
               
                   Haliotis rubra  (Jade 
                   
                   
                   
                   
                   
                   
               
               
                 tiger abaolone) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Haliotis midae 
                 
                   Hal m  1 
                 Yes 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Abalone) 
                   
                   
                   
                   
                   
                   
               
               
                   Helix aspersa  (Brown 
                   Hel as  1 
                 Yes 
                 Tropomyosin 
                 CAB3804 
                 O97192 
                 1188 
               
               
                 garden snail) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   odes pacificus 
                 
                   Tod p  1 
                 Yes 
                 Tropomyosin 
                 / 
                 / 
                 / 
               
               
                 (Japanese flying squid) 
                   
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Animalia Nematoda 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   Anisakis  simplex 
                   Ani s  1 
                 Yes 
                 unknown function, similar 
                 BAC77154 
                 Q7Z1K3 
                 1189 
               
               
                 (Herring worm) 
                   
                   
                 to Kunitz serine protease 
                   
                   
                   
               
               
                   
                   
                   
                 inhibitors 
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  2 
                 Yes 
                 Paramyosin 
                 AAF72796 
                 Q9NJA9 
                 1190 
               
               
                 (Herring worm) 
                   
                   
                   
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  3 
                 Yes 
                 Tropomyosin 
                 CAB93501 
                 Q9NAS5 
                 1191 
               
               
                 (Herring worm) 
                   
                   
                   
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  4 
                 Yes 
                 Cysteine protease inhibitor 
                 CAK50389 
                 Q14QT4 
                 1192 
               
               
                 (Herring worm) 
                   
                   
                   
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  5 
                 Yes 
                 SXP/RAL-2 family protein 
                 BAF43534 
                 A1IKL2 
                 1193 
               
               
                 (Herring worm) 
                   
                   
                   
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  6 
                 Yes 
                 Serine protease inhibitor 
                 BAF43535 
                 A1IKL3 
                 1194 
               
               
                 (Herring worm) 
                   
                   
                   
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  7 
                 Yes 
                 UA3-recognized allergen 
                 ABL77410 
                 A9XBJ8 
                 1195 
               
               
                 (Herring worm) 
                   
                   
                   
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  8 
                 Yes 
                 SXP/RAL-2 family 
                 BAF75681 
                 A7M6Q6 
                 1196 
               
               
                 (Herring worm) 
                   
                   
                 protein, isoform 1 
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  9 
                 Yes 
                 SXP/RAL-2 family protein 
                 ABV55106 
                 B2XCP1 
                 1197 
               
               
                 (Herring worm) 
                   
                   
                   
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  10 
                 Yes 
                 Protein with unknown 
                 ACZ95445 
                 D2K835 
                 1198 
               
               
                 (Herring worm) 
                   
                   
                 function 
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  11 
                 Yes 
                 Protein with unknown 
                 BAJ78220 
                 E9RFF3 
                 1199 
               
               
                 (Herring worm) 
                   
                   
                 function 
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  12 
                 Yes 
                 Protein with unknown 
                 BAJ78223 
                 E9RFF6 
                 1200 
               
               
                 (Herring worm) 
                   
                   
                 function 
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  13 
                 Yes 
                 Hemoglobin 
                 AFY98826 
                 K9USK2 
                 1201 
               
               
                 (Herring worm) 
                   
                   
                   
                   
                   
                   
               
               
                   Anisakis  simplex 
                   Ani s  14 
                 Yes 
                 3rd stage larval protein 
                 BAT62430 
                 A0A0S3Q267 
                 1202 
               
               
                 (Herring worm) 
                   
                   
                 unknown function 
                   
                   
                   
               
               
                 
                   Ascaris lumbricoides 
                 
                   Asc l  3 
                 No 
                 Tropomyosin 
                 ACN32322 
                 C0L3K2 
                 1203 
               
               
                 (Common roundworm) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ascaris lumbricoides 
                 
                   Asc l  13 
                 No 
                 lutathione S-transferase 
                 P46436 
                 P46436 
                 1204 
               
               
                 (Common roundworm) 
                   
                   
                 (GST) 
                   
                   
                   
               
               
                   Ascaris suum  (Pig 
                   Asc s  1 
                 No 
                 Polyprotein ABA-1 
                 AAC06015 
                 Q06811 
                 1205 
               
               
                 roundworm) 
                   
                   
                   
                   
                   
                   
               
               
                   Ascaris suum  (Pig 
                   Asc s  13 
                 No 
                 Glutathione transferase 
                 P46436 
                 P46436 
                 1204 
               
               
                 roundworm) 
                   
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Fungi Ascomycota 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 
                   Alternaria alternata 
                 
                 
                   Alt a 
                 
                 No 
                 / (major allergen) 
                 AAB47552 
                 P79085 
                 1206 
               
               
                 ( Alternaria  plant rot 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                 
                   Alt a 
                 
                 No 
                 / (major allergen) 
                 AAS75297 
                 Q6Q128 
                 1207 
               
               
                 ( Alternaria  plant rot 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  3 
                 No 
                 Heat shock protein 70 
                 AB48043 
                 P78983 
                 1208 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  4 
                 No 
                 Disulfide isomerase 
                 CAA58999 
                 Q00002 
                 1209 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  5 
                 No 
                 Ribosomal protein P2 
                 AAB48041 
                 P42037 
                 1210 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  6 
                 No 
                 Enolase 
                 AAG42022 
                 Q9HDT3 
                 1211 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  7 
                 No 
                 YCP4 protein 
                 CAA55069 
                 P42058 
                 1212 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  8 
                 No 
                 Mannitol dehydrogenase 
                 AAO91800 
                 P0C0Y4 
                 1213 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  10 
                 No 
                 Aldehyde dehydrogenase 
                 CAA55071 
                 P42041 
                 1214 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  12 
                 No 
                 Acid ribosomal protein P1 
                 CAA58998 
                 P49148 
                 1215 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  13 
                 No 
                 Glutathione-S-transferase 
                 AAR98813 
                 Q6R4B4 
                 1216 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  14 
                 No 
                 Manganese superoxide 
                 AGS80276 
                 P86254 
                 1217 
               
               
                 ( Alternaria  plant rot 
                   
                   
                 dismutase 
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Alternaria alternata 
                 
                   Alt a  15 
                 No 
                 Serine protease 
                 AHZ97469 
                 A0A0F6N3V8 
                 1218 
               
               
                 ( Alternaria  plant rot 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus flavus 
                 
                   Asp fl  13 
                 No 
                 Alkaline serine protease 
                 / 
                 / 
                 / 
               
               
                 (Cereal mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  1 
                 No 
                 Mitogillin family 
                 AAB07779 
                 P67875 
                 1219 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  2 
                 No 
                 / 
                 AAC69357 
                 P79017 
                 1220 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  3 
                 No 
                 Peroxysomal protein 
                 AAB95638 
                 O43099 
                 1221 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  4 
                 No 
                 / 
                 CAA04959 
                 O60024 
                 1222 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  5 
                 No 
                 Extracellular 
                 CAA83015 
                 P46075 
                 1223 
               
               
                 (Common mold) 
                   
                   
                 metalloproteinase 
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  6 
                 No 
                 Mn superoxide dismutase 
                 AAB60779 
                 Q92450 
                 1224 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  7 
                 No 
                 / 
                 CAA11255 
                 O42799 
                 1225 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  8 
                 No 
                 Ribosomal protein P2 
                 CAB64688 
                 Q9UUZ6 
                 1226 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  9 
                 No 
                 / 
                 CAA11266 
                 O42800 
                 1227 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  10 
                 No 
                 Aspartate protease 
                 CAA59419 
                 Q12547 
                 1228 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  11 
                 No 
                 Peptidyl-prolyl isomerase 
                 CAB44442 
                 Q9Y7F6 
                 1229 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  12 
                 No 
                 Heat shock protein P90 
                 AAB51544 
                 P40292 
                 1230 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  13 
                 No 
                 Alkaline serine protease 
                 CAA77666 
                 P28296 
                 1231 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  15 
                 No 
                 / 
                 CAA05149 
                 O60022 
                 1232 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  16 
                 No 
                 / 
                 CAAC61261 
                 O74682 
                 1233 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  17 
                 No 
                 / 
                 CAA12162 
                 O60025 
                 1234 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  18 
                 No 
                 Vacuolar serine protease 
                 CAA73782 
                 P87184 
                 1235 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  22 
                 No 
                 Enolase 
                 AAK49451 
                 Q96X30 
                 1236 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  23 
                 No 
                 L3 ribosomal protein 
                 AAM43909 
                 Q8NKF4 
                 1237 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  27 
                 No 
                 Cyclophilin 
                 CAI78448 
                 Q4WWX5 
                 1238 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  28 
                 No 
                 Thioredoxin 
                 CAI78449 
                 Q1RQJ1 
                 1239 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  29 
                 No 
                 Thioredoxin 
                 CAI78450 
                 Q4WV97 
                 1240 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus fumigatus 
                 
                   Asp f  34 
                 No 
                 PhiA cell wall protein 
                 CAM54066 
                 A4FSH5 
                 1241 
               
               
                 (Common mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus niger 
                 
                   Asp n  14 
                 No 
                 Beta-xylosidase 
                 AD13106 
                 O93933 
                 1242 
               
               
                 (Black mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus niger 
                 
                   Asp n  18 
                 No 
                 Vacuolar serine protease 
                 / 
                 / 
                 / 
               
               
                 (Black mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus niger 
                 
                   Asp n  25 
                 No 
                 3-phytase B 
                 AAA02934 
                 P34754 
                 1243 
               
               
                 (Black mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus oryzae 
                 
                   Asp o  13 
                 No 
                 Alkaline serine protease 
                 CAA35594 
                 P12547 
                 1244 
               
               
                 (Rice mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus oryzae 
                 
                   Asp o  21 
                 No 
                 TAKA-amylase A 
                 BAA00336 
                 P10529 
                 1245 
               
               
                 (Rice mold) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Aspergillus versicolor 
                 
                   Asp v  13 
                 No 
                 Extracellular alkaline 
                 ADE74975 
                 D5LGB3 
                 1246 
               
               
                   
                   
                   
                 serine protease 
                   
                   
                   
               
               
                 
                   Candida albicans 
                 
                   Cand a  1 
                 No 
                 Alcohol dehydrogenase 
                 CAA57342 
                 P43067 
                 1247 
               
               
                 (Common yeast) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Candida albicans 
                 
                   Cand a  3 
                 No 
                 Peroxysomal protein 
                 AAN11300 
                 Q6YK78 
                 1248 
               
               
                 (Common yeast) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Candida boidinii 
                 
                   Cand b  2 
                 No 
                 Peroxisomal membrane 
                 AAA34357 
                 P14292 
                 1249 
               
               
                 (Yeast) 
                   
                   
                 protein A 
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla c  9 
                 No 
                 Vacuolar serine protease 
                 ABQ59329 
                 B0L807 
                 1250 
               
               
                 
                   cladosporioides 
                 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla c  14 
                 No 
                 Transaldolase 
                 ADK47394 
                 G8Z407 
                 1251 
               
               
                 
                   cladosporioides 
                 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla h  2 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                   herbarum  (Fungus of 
                   
                   
                   
                   
                   
                   
               
               
                 plants) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla h  5 
                 No 
                 Acid ribosomal protein P2 
                 CAA55067 
                 P42039 
                 1252 
               
               
                   herbarum  (Fungus of 
                   
                   
                   
                   
                   
                   
               
               
                 plants) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla h  6 
                 No 
                 Enolase 
                 CAA55070 
                 P42040 
                 1253 
               
               
                   herbarum  (Fungus of 
                   
                   
                   
                   
                   
                   
               
               
                 plants) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla h  7 
                 No 
                 YCP4 protein 
                 CAA55068 
                 P42059 
                 1254 
               
               
                   herbarum  (Fungus of 
                   
                   
                   
                   
                   
                   
               
               
                 plants) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla h  8 
                 No 
                 Mannitol dehydrogenase 
                 AAO91801 
                 P0C0Y5 
                 1255 
               
               
                   herbarum  (Fungus of 
                   
                   
                   
                   
                   
                   
               
               
                 plants) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla h  9 
                 No 
                 Vacuolar serine protease 
                 AAX14379 
                 B7ZK61 
                 1256 
               
               
                   herbarum  (Fungus of 
                   
                   
                   
                   
                   
                   
               
               
                 plants) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla h  10 
                 No 
                 Aldehyde dehydrogenase 
                 CAA55072 
                 P40108 
                 1257 
               
               
                   herbarum  (Fungus of 
                   
                   
                   
                   
                   
                   
               
               
                 plants) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cladosporium 
                 
                   Cla h  12 
                 No 
                 Acid ribosomal protein P1 
                 CAA59463 
                 P50344 
                 1258 
               
               
                   herbarum  (Fungus of 
                   
                   
                   
                   
                   
                   
               
               
                 plants) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Curvularia lunata 
                 
                   Cur l  1 
                 No 
                 Serine protease 
                 / 
                 / 
                 / 
               
               
                 (Synonym: 
                   
                   
                   
                   
                   
                   
               
               
                   Cochliobolus lunatus ) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Curvularia lunata 
                 
                   Cur l  2 
                 No 
                 Enolase 
                 AAK67491 
                 Q96VP4 
                 1259 
               
               
                 (Synonym: 
                   
                   
                   
                   
                   
                   
               
               
                   Cochliobolus lunatus ) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Curvularia lunata 
                 
                   Cur l  3 
                 No 
                 Cytochrome c 
                 AAK67492 
                 Q96VP3 
                 1260 
               
               
                 (Synonym: 
                   
                   
                   
                   
                   
                   
               
               
                   Cochliobolus lunatus ) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Curvularia lunata 
                 
                   Cur l  4 
                 No 
                 Vacuolar serine protease 
                 ACF19589 
                 B3V0K8 
                 1261 
               
               
                 (Synonym: 
                   
                   
                   
                   
                   
                   
               
               
                   Cochliobolus lunatus ) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Epicoccum 
                 
                   Epi p  1 
                 No 
                 Serine protease 
                 P83340 
                 P83340 
                 1262 
               
               
                   purpurascens  (Soil 
                   
                   
                   
                   
                   
                   
               
               
                 fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Fusarium culmorum 
                 
                   Fus c  1 
                 No 
                 Ribosomal protein P2 
                 AAL79930 
                 Q8TFM9 
                 1263 
               
               
                 (N.A.) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Fusarium culmorum 
                 
                   Fus c  2 
                 No 
                 Thioredoxin-like protein 
                 AAL79931 
                 Q8TFM8 
                 1264 
               
               
                 (N.A.) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Fusarium proliferatum 
                 
                   Fus p  4 
                 No 
                 Transaldolase 
                 AHY02994 
                 / 
                 1265 
               
               
                 
                   Fusarium proliferatum 
                 
                   Fus p  9 
                 No 
                 Vacuolar serine protease 
                 AJA79001 
                 A0A0U1Y1N5 
                 1266 
               
               
                 
                   Penicillium 
                 
                   Pen b  13 
                 No 
                 Alkaline serine protease 
                 / 
                 / 
                 / 
               
               
                 
                   brevicompactum 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penicillium 
                 
                   Pen b  26 
                 No 
                 Acidic ribosomal prot. P1 
                 AAX11194 
                 Q49KL9 
                 1267 
               
               
                 
                   brevicompactum 
                 
                   
                   
                   
                   
                   
                   
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penicillium 
                 
                 
                   Pen ch 
                 
                 No 
                 Alkaline serine protease 
                 AAF23726 
                 Q9URR2 
                 1268 
               
               
                 
                   chrysogenum 
                 
                 13 
                   
                   
                   
                   
                   
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penicillium 
                 
                 
                   Pen ch 
                 
                 No 
                 Vacuolar serine protease 
                 AAF71379 
                 Q9P8G3 
                 1269 
               
               
                 
                   chrysogenum 
                 
                 18 
                   
                   
                   
                   
                   
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penicillium 
                 
                 
                   Pen ch 
                 
                 No 
                 N-acetyl-glucosaminidase 
                 AAB34785 
                 Q02352 
                 1270 
               
               
                 
                   chrysogenum 
                 
                 20 
                   
                   
                   
                   
                   
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penicillium 
                 
                 
                   Pen ch 
                 
                 No 
                 Calreticulin 
                 AAX45072 
                 Q2TL59 
                 1271 
               
               
                 
                   chrysogenum 
                 
                 31 
                   
                   
                   
                   
                   
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penicillium 
                 
                 
                   Pen ch 
                 
                 No 
                 / 
                 ABP04053 
                 B0L0W9 
                 1272 
               
               
                 
                   chrysogenum 
                 
                 33 
                   
                   
                   
                   
                   
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penicillium 
                 
                 
                   Pen ch 
                 
                 No 
                 Transaldolase 
                 ADK27483 
                 G8Z408 
                 1273 
               
               
                 
                   chrysogenum 
                 
                 35 
                   
                   
                   
                   
                   
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   lium citrinum 
                 
                   Pen c  3 
                 No 
                 Peroxysomal membrane 
                 AAD42074 
                 Q9Y8B8 
                 1274 
               
               
                 Penicillin) 
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   lium citrinum 
                 
                   Pen c  13 
                 No 
                 Alkaline serine protease 
                 Q9URH1 
                 Q9URH1 
                 1275 
               
               
                 (Penicillin) 
                   
                   
                 (segment 1) 
                   
                   
                   
               
               
                 
                   lium citrinum 
                 
                   Pen c  19 
                 No 
                 Heat shock protein P70 
                 AAB06397 
                 Q92260 
                 1276 
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   lium citrinum 
                 
                   Pen c  22 
                 No 
                 Enolase 
                 AAK51201 
                 Q96X46 
                 1277 
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   lium citrinum 
                 
                   Pen c  24 
                 No 
                 elongation factor 1 beta 
                 AAR17475 
                 Q69BZ7 
                 1278 
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   lium citrinum 
                 
                   Pen c  30 
                 No 
                 Catalase 
                 ABB89950 
                 Q2V6Q5 
                 1279 
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   lium citrinum 
                 
                   Pen c  32 
                 No 
                 Pectate lyase 
                 ABM60783 
                 A217W3 
                 1280 
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Penicillium crustosum 
                 
                   Pen cr  26 
                 No 
                 60S acidic ribosomal 
                 AEX34122 
                 H2E5X2 
                 1281 
               
               
                   
                   
                   
                 phosphoprotein P1 
                   
                   
                   
               
               
                 
                   Penicillium oxalicum 
                 
                   Pen o  18 
                 No 
                 vacuolar serine protease 
                 AAG44478 
                 Q9HF12 
                 1282 
               
               
                 (Penicillin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Stachybotrys chartarum 
                 
                   Sta c  3 
                 No 
                 Extracellular alkaline Mg- 
                 ACT37324 
                 C7E9W0 
                 1283 
               
               
                   
                   
                   
                 dependent 
                   
                   
                   
               
               
                   
                   
                   
                 exodesoxyribonuclease 
                   
                   
                   
               
               
                 
                   Trichophyton rubrum 
                 
                   Tri r  2 
                 No 
                 Putative secreted alkaline 
                 AAD52013 
                 Q9UW97 
                 1284 
               
               
                   
                   
                   
                 protease Alp1 
                   
                   
                   
               
               
                 
                   Trichophyton rubrum 
                 
                   Tri r  4 
                 No 
                 serine protease 
                 AAD52012 
                 Q9UW98 
                 1285 
               
               
                 
                   Trichophyton tonsurans 
                 
                   Tri t  1 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 
                   Trichophyton tonsurans 
                 
                   Tri t  4 
                 No 
                 Serine protease 
                 P80514 
                 P80514 
                 1286 
               
            
           
           
               
            
               
                 Fungi Basidiomycota and Zygomycota 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 
                   Coprinus comatus 
                 
                   Cop c  1 
                 No 
                 Leucine zipper protein 
                 CAB39376 
                 Q9Y7G3 
                 1287 
               
               
                 (Shaggy mane) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Coprinus comatus 
                 
                   Cop c  2 
                 No 
                 Thioredoxin 
                 CAB52130 
                 Q9UW02 
                 1288 
               
               
                 (Shaggy mane) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Coprinus comatus 
                 
                   Cop c  3 
                 No 
                 / 
                 CAB52131 
                 Q9UW01 
                 1289 
               
               
                 (Shaggy mane) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Coprinus comatus 
                 
                   Cop c  5 
                 No 
                 / 
                 CAB52132 
                 Q9UW00 
                 1290 
               
               
                 (Shaggy mane) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Coprinus comatus 
                 
                   Cop c  7 
                 No 
                 / 
                 CAB52133 
                 Q9UVZ9 
                 1291 
               
               
                 (Shaggy mane) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia furfur 
                 
                   Mala f  2 
                 No 
                 Peroxysomal membrane 
                 BAA32435 
                 P56577 
                 1292 
               
               
                 (Pityriasis versicolor 
                   
                   
                 protein 
                   
                   
                   
               
               
                 skin infection) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia furfur 
                 
                   Mala f  3 
                 No 
                 Peroxysomal membrane 
                 BAA32436 
                 P56578 
                 1293 
               
               
                 (Pityriasis versicolor 
                   
                   
                 protein 
                   
                   
                   
               
               
                 skin infection) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia furfur 
                 
                   Mala f  4 
                 No 
                 Mitochondrial malate 
                 AAD25927 
                 Q9Y750 
                 1294 
               
               
                 (Pityriasis versicolor 
                   
                   
                 dehydrogenase 
                   
                   
                   
               
               
                 skin infection) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                   Mala s  1 
                 No 
                 / 
                 CAA65341 
                 Q01940 
                 1295 
               
               
                 (Skin fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                   Mala s  5 
                 No 
                 / 
                 CAA09883 
                 O93969 
                 1296 
               
               
                 (Skin fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                   Mala s  6 
                 No 
                 Cyclophilin 
                 CAA09884 
                 O93970 
                 1297 
               
               
                 (Skin fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                   Mala s  7 
                 No 
                 / 
                 CAA09885 
                 O93971 
                 1298 
               
               
                 (Skin fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                   Mala s  8 
                 No 
                 / 
                 CAA09886 
                 O93972 
                 1299 
               
               
                 (Skin fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                   Mala s  9 
                 No 
                 / 
                 CAA09887 
                 O93973 
                 1300 
               
               
                 (Skin fungus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                 
                   Mala s 
                 
                 No 
                 heat shock protein 70 
                 CAD20981 
                 Q8TGH3 
                 1301 
               
               
                 (Skin fungus) 
                 10 
                   
                   
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                 
                   Mala s 
                 
                 No 
                 manganese superoxide 
                 CAD68071 
                 Q873M4 
                 1302 
               
               
                 (Skin fungus) 
                 11 
                   
                 dismutase 
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                 
                   Mala s 
                 
                 No 
                 glucose-methanol-choline 
                 CAI43283 
                 Q5GMY3 
                 1303 
               
               
                 (Skin fungus) 
                 12 
                   
                 (GMC) oxidoreductase 
                   
                   
                   
               
               
                 
                   Malassezia sympodialis 
                 
                 
                   Mala s 
                 
                 No 
                 Thioredoxin 
                 CAI78451 
                 Q1RQI9 
                 1304 
               
               
                 (Skin fungus) 
                 13 
                   
                   
                   
                   
                   
               
               
                 
                   Psilocybe cubensis 
                 
                   Psi c  1 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Magic mushroom) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Psilocybe cubensis 
                 
                   Psi c  2 
                 No 
                 Cyclophilin 
                 / 
                 / 
                 / 
               
               
                 (Magic mushroom) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Rhodotorula 
                 
                   Rho m  1 
                 No 
                 Enolase 
                 AAP30720 
                 Q870B9 
                 1305 
               
               
                   mucilaginosa  (Yeast) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Rhodotorula 
                 
                   Rho m  2 
                 No 
                 vacuolar serine protease 
                 AAT37679 
                 Q32ZM1 
                 1306 
               
               
                   mucilaginosa  (Yeast) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Schizophyllum 
                 
                   Sch c  1 
                 No 
                 Glucoamylase 
                 XP_003030591 
                 D8Q9M3 
                 1307 
               
               
                 
                   commune 
                 
                   
                   
                   
                   
                   
                   
               
               
                   Rhizopus oryzae  (Bread 
                   Rhi o  1 
                 No 
                 Aspartyl endopeptidase 
                 AIS82657 
                 I1CLC6 
                 1308 
               
               
                 mold) 
                   
                   
                   
                   
                   
                   
               
               
                   Rhizopus oryzae  (Bread 
                   Rhi o  2 
                 No 
                 Cyclophilin 
                 ALM24136 
                 / 
                 1309 
               
               
                 mold) 
                   
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Plantae Liliopsida 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 
                   Ananas comosus 
                 
                   Ana c  1 
                 Yes 
                 Profilin 
                 AAK54835 
                 Q94JN2 
                 1310 
               
               
                 (Pineapple) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ananas comosus 
                 
                   Ana c  2 
                 Yes 
                 Bromelain 
                 BAA21849 
                 O23791 
                 1311 
               
               
                 (Pineapple) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Anthoxanthum 
                 
                   Ant o  1 
                 No 
                 Beta-expansin 
                 Q7M1X6 
                 Q7M1X6 
                 1312 
               
               
                   odoratum  (Sweet vernal 
                   
                   
                   
                   
                   
                   
               
               
                 grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Asparagus officinalis 
                 
                   Aspa o  1 
                 Yes 
                 Non-specific lipid transfer 
                 / 
                 / 
                 / 
               
               
                 (Asparagus) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Cocos nucifera 
                 
                   Coc n  1 
                 No 
                 vicilin-like protein 
                 ALQ56981.1 
                 / 
                 1313 
               
               
                 (Coconut) 
                   
                   
                   
                   
                   
                   
               
               
                   Crocus sativus  (Saffron 
                   Cro s  1 
                 No 
                 / 
                 AAX93750 
                 Q29W25 
                 1314 
               
               
                 crocus) 
                   
                   
                   
                   
                   
                   
               
               
                   Crocus sativus  (Saffron 
                   Cro s  2 
                 No 
                 Profilin 
                 AAW81034 
                 Q5EF31 
                 1315 
               
               
                 crocus) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                 
                   Cyn d 
                 
                 No 
                 Beta-expansin 
                 AAB50734 
                 O04701 
                 1316 
               
               
                 (Bermuda grass) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                 
                   Cyn d 
                 
                 No 
                 Beta-expansin 
                 AAK96255 
                 Q947S7 
                 1317 
               
               
                 (Bermuda grass) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                 
                   Cyn d 
                 
                 No 
                 Beta-expansin 
                 AAL14077 
                 Q947S6 
                 1318 
               
               
                 (Bermuda grass) 
                 10202 
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                 
                   Cyn d 
                 
                 No 
                 Beta-expansin 
                 AAL14079 
                 Q947S4 
                 1319 
               
               
                 (Bermuda grass) 
                 1.0203 
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                 
                   Cyn d 
                 
                 No 
                 Beta-expansin 
                 AAF80379 
                 Q9FVM0 
                 1320 
               
               
                 (Bermuda grass) 
                 1.0204 
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                   Cyn d  7 
                 No 
                 Polcalcin 
                 CAA62634 
                 P94092 
                 1321 
               
               
                 (Bermuda grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                   Cyn d  12 
                 No 
                 Profilin 
                 CAA69670 
                 O04725 
                 1322 
               
               
                 (Bermuda grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                   Cyn d  15 
                 No 
                 pollen allergen(/) 
                 AAP80171 
                 Q7XYF2 
                 1323 
               
               
                 (Bermuda grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                 
                   Cyn d 
                 
                 No 
                 enolase 
                 / 
                 / 
                 / 
               
               
                 (Bermuda grass) 
                 22w 
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                   Cyn d  23 
                 No 
                 / (pollen allergen) 
                 AAP80170 
                 Q7XYF3 
                 1324 
               
               
                 (Bermuda grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cynodon dactylon 
                 
                   Cyn d  24 
                 No 
                 Pathogenesis-related 
                 AAU15051 
                 Q647J6 
                 1325 
               
               
                 (Bermuda grass) 
                   
                   
                 protein PR-1 
                   
                   
                   
               
               
                 
                   Dactylis glomerata 
                 
                   Dac g  1 
                 No 
                 Beta-expansin 
                 Q7M1X8 
                 Q7M1X8 
                 1326 
               
               
                 (Orchard grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dactylis glomerata 
                 
                   Dac g  2 
                 No 
                 / 
                 AAB23303 
                 Q41183 
                 1327 
               
               
                 (Orchard grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dactylis glomerata 
                 
                   Dac g  3 
                 No 
                 / 
                 AAB42200 
                 P93124 
                 1328 
               
               
                 (Orchard grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dactylis glomerata 
                 
                   Dac g  4 
                 No 
                 / 
                 P82946 
                 P82946 
                 1329 
               
               
                 (Orchard grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Dactylis glomerata 
                 
                   Dac g  5 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Orchard grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Festuca pratensis 
                 
                   Fes p  4 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Meadow fescue) 
                   
                   
                   
                   
                   
                   
               
               
                   Holcus lanatus  (Velvet 
                   Hol l  1 
                 No 
                 Beta-expansin 
                 CAA81610 
                 P43216 
                 1330 
               
               
                 grass) 
                   
                   
                   
                   
                   
                   
               
               
                   Holcus lanatus  (Velvet 
                 
                   Hol l 
                 
                 No 
                 Group V allergen 
                 CAB10765 
                 O23972 
                 1331 
               
               
                 grass) 
                 5.0101 
                   
                   
                   
                   
                   
               
               
                   Holcus lanatus  (Velvet 
                 
                   Hol l 
                 
                 No 
                 Group V allergen 
                 CAB10766 
                 O23971 
                 1332 
               
               
                 grass) 
                 5.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Hordeum vulgare 
                 
                   Hor v  5 
                 No 
                 / 
                 AAB41585 
                 O04828 
                 1333 
               
               
                 (Barley) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Hordeum vulgare 
                 
                   Hor v  12 
                 No 
                 Profilin 
                 AAA92503 
                 P52184 
                 1334 
               
               
                 (Barley) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Hordeum vulgare 
                 
                   Hor v  15 
                 No 
                 Alpha-amylase inhibitor 
                 CAA45085 
                 P16968 
                 1335 
               
               
                 (Barley) 
                   
                   
                 BMAI-1 precursor 
                   
                   
                   
               
               
                 
                   Hordeum vulgare 
                 
                   Hor v  16 
                 No 
                 Alpha-amylase 
                 / 
                 / 
                 / 
               
               
                 (Barley) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Hordeum vulgare 
                 
                   Hor v  17 
                 No 
                 Beta-amylase 
                 / 
                 / 
                 / 
               
               
                 (Barley) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Hordeum vulgare 
                 
                   Hor v  20 
                 No 
                 Gamma-hordein 3 
                 CAA51204 
                 P80198 
                 1336 
               
               
                 (Barley) 
                   
                   
                   
                   
                   
                   
               
               
                   Lolium perenne  (Rye 
                 
                   Lol p 
                 
                 No 
                 Beta-expansin 
                 AAA63279 
                 P14946 
                 1337 
               
               
                 grass) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                   Lolium perenne  (Rye 
                 
                   Lol p 
                 
                 No 
                 Beta-expansin 
                 CAB63699 
                 Q9SC98 
                 1338 
               
               
                 grass) 
                 1.0103 
                   
                   
                   
                   
                   
               
               
                   Lolium perenne  (Rye 
                   Lol p  2 
                 No 
                 / 
                 P14947 
                 P14947 
                 1339 
               
               
                 grass) 
                   
                   
                   
                   
                   
                   
               
               
                   Lolium perenne  (Rye 
                   Lol p  3 
                 No 
                 / 
                 P14948 
                 P14948 
                 1340 
               
               
                 grass) 
                   
                   
                   
                   
                   
                   
               
               
                   Lolium perenne  (Rye 
                   Lol p  4 
                 No 
                 / 
                 CAH92637 
                 Q5TIW3 
                 1341 
               
               
                 grass) 
                   
                   
                   
                   
                   
                   
               
               
                   Lolium perenne  (Rye 
                   Lol p  5 
                 No 
                 / 
                 AAA33405 
                 Q40237 
                 1342 
               
               
                 grass) 
                   
                   
                   
                   
                   
                   
               
               
                   Lolium perenne  (Rye 
                   Lol p  11 
                 No 
                 Ole e 1-related protein 
                 Q7M1X5 
                 Q7M1X5 
                 1343 
               
               
                 grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Musa acuminata 
                 
                   Mus a  1 
                 Yes 
                 Profilin 
                 AAK54834 
                 Q94JN3 
                 1344 
               
               
                 (Banana) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Musa acuminata 
                 
                   Mus a  2 
                 Yes 
                 Class 1 chitinase 
                 CAC81811 
                 Q8VXF1 
                 1345 
               
               
                 (Banana) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Musa acuminata 
                 
                   Mus a  3 
                 Yes 
                 Non-specific lipid transfer 
                 P86333 
                 P86333 
                 1346 
               
               
                 (Banana) 
                   
                   
                 protein type 1 (nsLTP1) 
                   
                   
                   
               
               
                 
                   Musa acuminata 
                 
                   Mus a  4 
                 Yes 
                 Thaumatin-like protein 
                 1Z3Q_A 
                 / 
                 1347 
               
               
                 (Banana) 
                   
                   
                 (Chain A) 
                   
                   
                   
               
               
                 
                   Musa acuminata 
                 
                   Mus a  5 
                 Yes 
                 Beta-1,3-glucanase 
                 / 
                 / 
                 / 
               
               
                 (Banana) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Musa acuminata 
                 
                   Mus a  6 
                 Yes 
                 ascorbate peroxidase 
                 / 
                 / 
                 / 
               
               
                 (Banana) 
                   
                   
                   
                   
                   
                   
               
               
                   Oryza sativa  (Rice) 
                   Ory s  1 
                 No 
                 Beta-expansin 
                 AAA86533 
                 Q40638 
                 1348 
               
               
                   Oryza sativa  (Rice) 
                   Ory s  12 
                 Yes 
                 Profilin A 
                 AAG32056 
                 Q9FUD1 
                 1349 
               
               
                 
                   Paspalum notatum 
                 
                   Pas n  1 
                 No 
                 Beta expansin 
                 ACA23876 
                 B8PYF3 
                 1350 
               
               
                 (Bahia grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phalaris aquatica 
                 
                   Pha a  1 
                 No 
                 Beta-expansin 
                 AAB35984 
                 Q41260 
                 1351 
               
               
                 (Canary grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phalaris aquatica 
                 
                   Pha a  5 
                 No 
                 / 
                 P56164 
                 P56164 
                 1352 
               
               
                 (Canary grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 Beta-expansin 
                 CAA81613 
                 Q40967 
                 1353 
               
               
                 (Timothy) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 Beta-expansin 
                 CAA55390 
                 P43213 
                 1354 
               
               
                 (Timothy) 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                   Phl p  2 
                 No 
                 Grass group II/III 
                 CAA53529 
                 P43214 
                 1355 
               
               
                 (Timothy) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 Berberine bridge enzyme 
                 CAD54670 
                 Q5ZQK5 
                 1356 
               
               
                 (Timothy) 
                 4.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 Berberine bridge enzyme 
                 CAD54671 
                 Q5ZQK4 
                 1357 
               
               
                 (Timothy) 
                 4.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 CAA52753 
                 Q40960 
                 1358 
               
               
                 (Timothy) 
                 5.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 CAA50281 
                 Q40962 
                 1359 
               
               
                 (Timothy) 
                 5.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 AAC25994 
                 O81341 
                 1360 
               
               
                 (Timothy) 
                 5.0103 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 CAB05372 
                 P93467 
                 1361 
               
               
                 (Timothy) 
                 5.0104 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 AAC16525 
                 O65318 
                 1362 
               
               
                 (Timothy) 
                 5.0105 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 AAC16526 
                 O65319 
                 1363 
               
               
                 (Timothy) 
                 5.0106 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 AAC16528 
                 O65321 
                 1364 
               
               
                 (Timothy) 
                 5.0108 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 CAD87529 
                 Q84UI2 
                 1365 
               
               
                 (Timothy) 
                 5.0109 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 CAA81609 
                 Q40963 
                 1366 
               
               
                 (Timothy) 
                 5.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 CAB05371 
                 P93466 
                 1367 
               
               
                 (Timothy) 
                 5.0202 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 AAC25995 
                 O81342 
                 1368 
               
               
                 (Timothy) 
                 5.0203 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 AAC25997 
                 O81343 
                 1369 
               
               
                 (Timothy) 
                 5.0206 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 AAC25998 
                 O81344 
                 1370 
               
               
                 (Timothy) 
                 5.0207 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 CAA81608 
                 P43215 
                 1371 
               
               
                 (Timothy) 
                 6.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 / 
                 CAA76556 
                 O65868 
                 1372 
               
               
                 (Timothy) 
                 6.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                   Phl p  7 
                 No 
                 Polcalcin 
                 CAA76887 
                 O82040 
                 1373 
               
               
                 (Timothy) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                   Phl p  11 
                 No 
                 Ole e 1-related protein 
                 AAN32987 
                 Q8H6L7 
                 1374 
               
               
                 (Timothy) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 Profilin-1 
                 CAA54686 
                 P35079 
                 1375 
               
               
                 (Timothy) 
                 12.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 Profilin-2 
                 CAA70608 
                 O24650 
                 1376 
               
               
                 (Timothy) 
                 12.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                 
                   Phl p 
                 
                 No 
                 Profilin-3 
                 CAA70609 
                 O24282 
                 1377 
               
               
                 (Timothy) 
                 12.0103 
                   
                   
                   
                   
                   
               
               
                 
                   Phleum pratense 
                 
                   Phl p  13 
                 No 
                 Polygalacturonase 
                 CAB42886 
                 Q9XG86 
                 1378 
               
               
                 (Timothy) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phoenix dactylifera 
                 
                   Pho d  2 
                 No 
                 Profilin 
                 CAD10390 
                 Q8L5D8 
                 1379 
               
               
                 (Date palm) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Poa pratensis 
                 
                   Poa p  1 
                 No 
                 Beta-expansin 
                 CAA10520 
                 Q9ZP03 
                 1380 
               
               
                 (Kentucky blue grass) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Poa pratensis 
                 
                   Poa p  5 
                 No 
                 / (pollen allergen) 
                 AAG42254 
                 Q9FPR0 
                 1381 
               
               
                 (Kentucky blue grass) 
                   
                   
                   
                   
                   
                   
               
               
                   Secale cereale  (Rye) 
                   Sec c  5 
                 No 
                 Group 5 grass pollen 
                 CBG76811 
                 F4MJM3 
                 1382 
               
               
                   
                   
                   
                 allergen 
                   
                   
                   
               
               
                   Secale cereale  (Rye) 
                 
                   Sec c 
                 
                 Yes 
                 Gamma-70 SECALIN 
                 Q9S8B0 
                 Q9S8B0 
                 1383 
               
               
                   
                 20.0101 
                   
                 isoform S10-12 
                   
                   
                   
               
               
                   
                   
                   
                 (COELIAC 
                   
                   
                   
               
               
                   
                   
                   
                 immunoreactive protein) 
                   
                   
                   
               
               
                   Secale cereale  (Rye) 
                 
                   Sec c 
                 
                 Yes 
                 Gamma-35 SECALIN 
                 Q9S8A7 
                 Q9S8A7 
                 1384 
               
               
                   
                 20.0201 
                   
                 isoform P13-14 
                   
                   
                   
               
               
                   
                   
                   
                 (COELIAC 
                   
                   
                   
               
               
                   
                   
                   
                 immunoreactive protein) 
                   
                   
                   
               
               
                   Secale cereale  (Rye) 
                   Sec c  38 
                 No 
                 Dimeric alpha- 
                 Q9S8H2 
                 Q9S8H2 
                 1385 
               
               
                   
                   
                   
                 amylase/trypsin inhibitor 
                   
                   
                   
               
               
                 
                   Sorghum halepense 
                 
                 
                   Sor h 
                 
                 No 
                 Beta-expansin 
                 AIL01316 
                 C5WMS3 
                 1386 
               
               
                 (Johnson grass) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Sorghum halepense 
                 
                 
                   Sor h 
                 
                 No 
                 Beta-expansin 
                 AIL01317 
                 A0A077B4J2 
                 1387 
               
               
                 (Johnson grass) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Sorghum halepense 
                 
                 
                   Sor h 
                 
                 No 
                 Expansin-like protein; 
                 AIL01318 
                 A0A077B7S9 
                 1388 
               
               
                 (Johnson grass) 
                 2.0101 
                   
                 grass pollen group 2 
                   
                   
                   
               
               
                   
                   
                   
                 allergen 
                   
                   
                   
               
               
                 
                   Sorghum halepense 
                 
                 
                   Sor h 
                 
                 No 
                 Expansin-like protein; 
                 AIL01319 
                 A0A077B2S0 
                 1389 
               
               
                 (Johnson grass) 
                 2.0201 
                   
                 grass pollen group 2 
                   
                   
                   
               
               
                   
                   
                   
                 allergen 
                   
                   
                   
               
               
                 
                   Sorghum halepense 
                 
                 
                   Sor h 
                 
                 No 
                 Exopolygalacturonase 
                 AIL01320 
                 A0A077B155 
                 1390 
               
               
                 (Johnson grass) 
                 13.0101 
                   
                 (Glycosyl hydrolase 28) 
                   
                   
                   
               
               
                 
                   Sorghum halepense 
                 
                 
                   Sor h 
                 
                 No 
                 Exopolygalacturonase 
                 AIL01321 
                 A0A077B569 
                 1391 
               
               
                 (Johnson grass) 
                 13.0201 
                   
                 (Glycosyl hydrolase 28) 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                 
                   Tri a 
                 
                 No 
                 Profilin-1 
                 CAA61943 
                 P49232 
                 1392 
               
               
                 (Wheat) 
                 12.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                 
                   Tri a 
                 
                 No 
                 Profilin-2 
                 CAA61944 
                 P49233 
                 1393 
               
               
                 (Wheat) 
                 12.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                 
                   Tri a 
                 
                 No 
                 Profilin-3 
                 CAA61945 
                 P49234 
                 1394 
               
               
                 (Wheat) 
                 12.0103 
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                 
                   Tri a 
                 
                 No 
                 Profilin-4 
                 CAQ57979 
                 B6EF35 
                 1395 
               
               
                 (Wheat) 
                 12.0104 
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  14 
                 Yes 
                 Non-specific lipid transfer 
                 CAY54133 
                 D2T2K2 
                 1396 
               
               
                 (Wheat) 
                   
                   
                 protein 1 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  15 
                 No 
                 Monomeric alpha-amylase 
                 CBA13560 
                 D2TGC3 
                 1397 
               
               
                 (Wheat) 
                   
                   
                 inhibitor 0.28 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  18 
                 Yes 
                 Agglutinin isolectin 1 
                 AAA34256 
                 P10968 
                 1398 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  19 
                 Yes 
                 Omega-5 gliadin, seed 
                 BAE20328 
                 Q402I5 
                 1399 
               
               
                 (Wheat) 
                   
                   
                 storage protein 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  20 
                 Yes 
                 Gamma gliadin 
                 BAN29066 
                 Q9SYX8 
                 1400 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  21 
                 No 
                 alpha-beta-gliadin 
                 CAY54134 
                 D2T2K3 
                 1401 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  25 
                 Yes 
                 Thioredoxin H 
                 CAB96931 
                 Q9LDX4 
                 1402 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                 
                   Tri a 
                 
                 Yes 
                 High molecular weight 
                 CAA31395 
                 P10388 
                 1403 
               
               
                 (Wheat) 
                 26.0101 
                   
                 glutenin subunit Dx5 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                 
                   Tri a 
                 
                 Yes 
                 High molecular weight 
                 AAZ23584 
                 Q45R38 
                 1404 
               
               
                 (Wheat) 
                 26.0201 
                   
                 glutenin subunit Bx7 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  27 
                 No 
                 Thiol reductase homologue 
                 BAC76688 
                 Q7Y1Z2 
                 1405 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  28 
                 No 
                 Dimeric alpha-amylase 
                 CAI84642 
                 Q4W0V7 
                 1406 
               
               
                 (Wheat) 
                   
                   
                 inhibitor 0.19 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                 
                   Tri a 
                 
                 No 
                 Tetrameric alpha-amylase 
                 CAZ76052 
                 C7C4X0 
                 1407 
               
               
                 (Wheat) 
                 29.0101 
                   
                 inhibitor CM1 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                 
                   Tri a 
                 
                 No 
                 Tetrameric alpha-amylase 
                 CBA13559 
                 D2TGC2 
                 1408 
               
               
                 (Wheat) 
                 29.0201 
                   
                 inhibitor CM2 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  30 
                 No 
                 Tetrameric alpha-amylase 
                 CAA35597 
                 P17314 
                 1409 
               
               
                 (Wheat) 
                   
                   
                 inhibitor CM3 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  31 
                 No 
                 Triosephosphate-isomerase 
                 CAC14917 
                 Q9FS79 
                 1410 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  32 
                 No 
                 1-cys-peroxiredoxin 
                 AAQ74769 
                 Q6W8Q2 
                 1411 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  33 
                 No 
                 Serpin 
                 CAB52710 
                 Q9ST57 
                 1412 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  34 
                 No 
                 Glyceraldehyde-3- 
                 CAZ76054 
                 C7C4X1 
                 1413 
               
               
                 (Wheat) 
                   
                   
                 phosphate-dehydrogenase 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  35 
                 No 
                 Dehydrin 
                 CAY85463 
                 D2TE72 
                 1414 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  36 
                 Yes 
                 Low molecular weight 
                 AEH31546 
                 B2Y2Q7 
                 1415 
               
               
                 (Wheat) 
                   
                   
                 glutenin GluB3-23 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  37 
                 Yes 
                 Alpha purothionin 
                 CAA65313 
                 Q9T0P1 
                 1416 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  39 
                 No 
                 Serine protease inhibitor- 
                 CCK33471 
                 J7QW61 
                 1417 
               
               
                 (Wheat) 
                   
                   
                 like protein 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  40 
                 No 
                 oform/methanol-soluble 
                 CAA42453.1 
                 Q41540 
                 1418 
               
               
                 (Wheat) 
                   
                   
                 (CM) 17 protein [alpha 
                   
                   
                   
               
               
                   
                   
                   
                 amylase inhibitor] 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  41 
                 Yes 
                 Mitochondrial ubiquitin 
                 AKJ77988.1 
                 A0A0G3F2P1 
                 1419 
               
               
                 (Wheat) 
                   
                   
                 ligase activator of NFKB 1 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  42 
                 Yes 
                 Hypothetical protein from 
                 AKJ77986.1 
                 A0A0G3F2F5 
                 1420 
               
               
                 (Wheat) 
                   
                   
                 cDNA 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  43 
                 Yes 
                 Hypothetical protein from 
                 AKJ77987.1 
                 A0A0G3F5F7 
                 1421 
               
               
                 (Wheat) 
                   
                   
                 cDNA 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  44 
                 Yes 
                 Endosperm transfer cell 
                 AKJ77990.1 
                 A0A0G3F720 
                 1422 
               
               
                 (Wheat) 
                   
                   
                 specific PR60 precursor 
                   
                   
                   
               
               
                 
                   Triticum aestivum 
                 
                   Tri a  45 
                 Yes 
                 Elongation factor 1 (EIF1) 
                 AKJ77985.1 
                 A0A0G3F715 
                 1423 
               
               
                 (Wheat) 
                   
                   
                   
                   
                   
                   
               
               
                   Zea mays  (Maize) 
                   Zea m  1 
                 No 
                 Beta-expansin 
                 AAA33496 
                 Q07154 
                 1424 
               
               
                   Zea mays  (Maize) 
                   Zea m  8 
                 Yes 
                 Chitinase 
                 ACX37090 
                 P29022 
                 1425 
               
               
                   Zea mays  (Maize) 
                 
                   Zea m 
                 
                 No 
                 Profilin-1 
                 CAA51718 
                 P35081 
                 1426 
               
               
                   
                 12.0101 
                   
                   
                   
                   
                   
               
               
                   Zea mays  (Maize) 
                 
                   Zea m 
                 
                 No 
                 Profilin-2 
                 CAA51719 
                 P35082 
                 1427 
               
               
                   
                 12.0102 
                   
                   
                   
                   
                   
               
               
                   Zea mays  (Maize) 
                 
                   Zea m 
                 
                 No 
                 Profilin-3 
                 CAA51720 
                 P35083 
                 1428 
               
               
                   
                 12.0103 
                   
                   
                   
                   
                   
               
               
                   Zea mays  (Maize) 
                 
                   Zea m 
                 
                 No 
                 Profilin-4 
                 AAB86960 
                 O22655 
                 1429 
               
               
                   
                 12.0104 
                   
                   
                   
                   
                   
               
               
                   Zea mays  (Maize) 
                 
                   Zea m 
                 
                 No 
                 Profilin-5 
                 AAG35601 
                 Q9FR39 
                 1430 
               
               
                   
                 12.0105 
                   
                   
                   
                   
                   
               
               
                   Zea mays  (Maize) 
                 
                   Zea m 
                 
                 Yes 
                 Non-specific lipid-transfer 
                 AAA33493 
                 P19656-1 
                 1431 
               
               
                   
                 14.0101 
                   
                 protein, isoform 1 
                   
                   
                   
               
               
                   Zea mays  (Maize) 
                 
                   Zea m 
                 
                 Yes 
                 Non-specific lipid-transfer 
                 AAA33494 
                 P19656-2 
                 1432 
               
               
                   
                 14.0102 
                   
                 protein, isoform 2 
                   
                   
                   
               
               
                   Zea mays  (Maize) 
                   Zea m  25 
                 No 
                 thioredoxin 
                 CAI64400 
                 Q4W1F7 
                 1433 
               
            
           
           
               
            
               
                 Plantae Magnoliopsida 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 
                   Acacia farnesiana 
                 
                   Aca f  1 
                 No 
                 Ole e 1-like protein 
                 AKV7266.1 
                 A0A0K1SC24 
                 1434 
               
               
                 ( Vachellia farnesiana ) 
                   
                   
                   
                   
                   
                   
               
               
                 (Needle bush) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Acacia farnesiana 
                 
                   Aca f  2 
                 No 
                 profilin 
                 AIV43662.1 
                 A0A0A0RCW1 
                 1435 
               
               
                 ( Vachellia farnesiana ) 
                   
                   
                   
                   
                   
                   
               
               
                 (Needle bush) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia chinensis 
                 
                 
                   Act c 
                 
                 Yes 
                 Kiwellin 
                 P85261.1 
                 P85261 
                 1436 
               
               
                 (Gold Kiwi fruit) 
                 5.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia chinensis 
                 
                 
                   Act c 
                 
                 Yes 
                 Kiwellin 
                 AGC39168.1 
                 L7TT83 
                 1437 
               
               
                 (Gold Kiwi fruit) 
                 5.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia chinensis 
                 
                   Act c  8 
                 Yes 
                 Pathogenesis-related 
                 CAM31908.1 
                 D1YSM4 
                 1438 
               
               
                 (Gold Kiwi fruit) 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Actinidia chinensis 
                 
                   Act c  10 
                 Yes 
                 Non-specific lipid-transfer 
                 P85204 
                 P85204 
                 1439 
               
               
                 (Gold Kiwi fruit) 
                   
                   
                 protein 1 
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  1 
                 Yes 
                 Cysteine protease 
                 CAA34486 
                 P00785 
                 1440 
               
               
                 (Green Kiwi fruit) 
                   
                   
                 (actinidin) 
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  2 
                 Yes 
                 Thaumatin-like protein 
                 CAI38795 
                 P81370 
                 1441 
               
               
                 (Green Kiwi fruit) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  3 
                 Yes 
                 / 
                 P85063 
                 P85063 
                 1442 
               
               
                 (Green Kiwi fruit) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  4 
                 Yes 
                 Phytocystatin 
                 AAR92223 
                 Q6TPK4 
                 1443 
               
               
                 (Green Kiwi fruit) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  5 
                 Yes 
                 Kiwellin 
                 P84527 
                 P84527 
                 1444 
               
               
                 (Green Kiwi fruit) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  6 
                 Yes 
                 Pectin methylesterase 
                 BAC54964 
                 P83326 
                 1445 
               
               
                 (Green Kiwi fruit) 
                   
                   
                 inhibitor 
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  7 
                 Yes 
                 Pectin methylesterase 
                 P85076 
                 P85076 
                 1446 
               
               
                 (Green Kiwi fruit) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  8 
                 Yes 
                 Pathogenesis-related 
                 CAM31909 
                 D1YSM5 
                 1447 
               
               
                 (Green Kiwi fruit) 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  9 
                 Yes 
                 Profilin 
                 / 
                 / 
                 / 
               
               
                 (Green Kiwi fruit) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                 
                   Act d 
                 
                 Yes 
                 Non-specific lipid-transfer 
                 P86137 
                 P86137 
                 1448 
               
               
                 (Green Kiwi fruit) 
                 10.0101 
                   
                 protein 1 
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                 
                   Act d 
                 
                 Yes 
                 Non-specific lipid-transfer 
                 P85206 
                 P85206 
                 1449 
               
               
                 (Green Kiwi fruit) 
                 10.0201 
                   
                 protein 2 
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  11 
                 Yes 
                 Major latex 
                 P85524 
                 P85524 
                 1450 
               
               
                 (Green Kiwi fruit) 
                   
                   
                 protein/ripening-related 
                   
                   
                   
               
               
                   
                   
                   
                 protein (MLP/RRP),  Bet v   
                   
                   
                   
               
               
                   
                   
                   
                 1 family member 
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                 
                   Act d 
                 
                 Yes 
                 Cupin, 11S globulin 
                 / 
                 C0HJF9.1 
                 1451 
               
               
                 (Green Kiwi fruit) 
                 12.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                 
                   Act d 
                 
                 Yes 
                 Cupin, 11S globulin 
                 ABB77213 
                 / 
                 1452 
               
               
                 (Green Kiwi fruit) 
                 12.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Actinidia deliciosa 
                 
                   Act d  13 
                 Yes 
                 2S albumin 
                 / 
                 / 
                 / 
               
               
                 (Green Kiwi fruit) 
                   
                   
                   
                   
                   
                   
               
               
                   Alnus glutinosa  (Alder) 
                   Aln g  1 
                 No 
                 Pathogenesis-related 
                 AAB24432 
                 P38948 
                 1453 
               
               
                   
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Alnus glutinosa  (Alder) 
                   Aln g  4 
                 No 
                 Polcalcin 
                 CAA76831 
                 O81701 
                 1454 
               
               
                 
                   Amaranthus retroflexus 
                 
                   Ama r  1 
                 No 
                 Ole e 1- like protein 
                 AKV72168 
                 A0A0K1SC10 
                 1455 
               
               
                 (Redroot pigweed) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Amaranthus retroflexus 
                 
                   Ama r  2 
                 No 
                 Profilin 
                 ACP43298 
                 C3W2Q7 
                 1456 
               
               
                 (Redroot pigweed) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 5 
                 AAA32665 
                 P27759 
                 1457 
               
               
                 (Short ragweed) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 1 
                 AAA32666 
                 P27760 
                 1458 
               
               
                 (Short ragweed) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 
                 CBW30987 
                 E1XUL3 
                 1459 
               
               
                 (Short ragweed) 
                 1.0202 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 2 
                 AAA32668 
                 P27761 
                 1460 
               
               
                 (Short ragweed) 
                 1.0301 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 2 
                 / 
                 P27761 
                 1460 
               
               
                 (Short ragweed) 
                 1.0302 
                   
                   
                   
                 (variant 
                   
               
               
                   
                   
                   
                   
                   
                 L48Y) 
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 2 
                 AAA32669 
                 P27761 
                 1460 
               
               
                 (Short ragweed) 
                 1.0303 
                   
                   
                   
                 (variant 
                   
               
               
                   
                   
                   
                   
                   
                 H392R) 
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 
                 CBW30988 
                 E1XUL4 
                 1461 
               
               
                 (Short ragweed) 
                 1.0304 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 
                 CBW30989 
                 E1XUL5 
                 1462 
               
               
                 (Short ragweed) 
                 1.0305 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 3 
                 AAA32670 
                 P28744 
                 1463 
               
               
                 (Short ragweed) 
                 1.0401 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 
                 CBW30993 
                 E1XUL9 
                 1464 
               
               
                 (Short ragweed) 
                 1.0402 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 4 
                 AAA32671 
                 P27762 
                 1465 
               
               
                 (Short ragweed) 
                 1.0501 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Pectate lyase 
                 CBW30995 
                 E1XUM1 
                 1466 
               
               
                 (Short ragweed) 
                 1.0502 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                   Amb a  3 
                 No 
                 Plastocyanine 
                 P00304 
                 P00304 
                 1467 
               
               
                 (Short ragweed) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                   Amb a  4 
                 No 
                 Defensin-like protein 
                 CBK52317 
                 D4IHC0 
                 1468 
               
               
                 (Short ragweed) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                   Amb a  5 
                 No 
                 / 
                 P02878 
                 P02878 
                 1469 
               
               
                 (Short ragweed) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                   Amb a  6 
                 No 
                 Non-specific lipid transfer 
                 AAB51146 
                 O04004 
                 1470 
               
               
                 (Short ragweed) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                   Amb a  7 
                 No 
                 Plastocyanin 
                 / 
                 / 
                 / 
               
               
                 (Short ragweed) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Profilin 
                 AAX77687 
                 Q2KN24 
                 1471 
               
               
                 (Short ragweed) 
                 8.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Profilin 
                 AAX77688 
                 Q2KN23 
                 1472 
               
               
                 (Short ragweed) 
                 8.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Polcalcin 9Calcium- 
                 AAX77684 
                 Q2KN27 
                 1473 
               
               
                 (Short ragweed) 
                 9.0101 
                   
                 binding protein isoallergen 1) 
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Calcium-binding protein 
                 AAX77685 
                 Q2KN26 
                 1474 
               
               
                 (Short ragweed) 
                 9.0102 
                   
                 isoallergen 2 
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Polcalcin-like protein (4 
                 AAX77686 
                 Q2KN25 
                 1475 
               
               
                 (Short ragweed) 
                 10 
                   
                 EF-hands) 
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Cysteine protease 
                 AHA56102 
                 V5LU01 
                 1476 
               
               
                 (Short ragweed) 
                 11 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Enolase 
                 ANZ22901.1 
                 A0A1B2H9Q1 
                 1477 
               
               
                 (Short ragweed) 
                 12.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia artemisiifolia 
                 
                 
                   Amb a 
                 
                 No 
                 Enolase 
                 ANZ22900 
                 A0A1B2H9Q5 
                 1478 
               
               
                 (Short ragweed) 
                 12.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia psilostachya 
                 
                 
                   Amb p 
                 
                 No 
                 / (pollen allergen) 
                 AAA20065 
                 P43174 
                 1479 
               
               
                 (Western ragweed) 
                 5.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Ambrosia psilostachya 
                 
                 
                   Amb p 
                 
                 No 
                 / (pollen Allergen) 
                 AAA20064 
                 P43175 
                 1480 
               
               
                 (Western ragweed) 
                 5.0201 
                   
                   
                   
                   
                   
               
               
                   Ambrosia trifida  (Giant 
                   Amb t  5 
                 No 
                 / (pollen allergen) 
                 CAA39726 
                 P10414 
                 1481 
               
               
                 ragweed) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Anacardium 
                 
                   Ana  0 
                 Yes 
                 Vicilin-like protein 
                 AAM73730 
                 Q8L5L5 
                 1482 
               
               
                   occidentale  (Cashew) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Anacardium 
                 
                 
                   Ana o 
                 
                 Yes 
                 Vicilin-like protein 
                 AAM73729 
                 Q8L5L6 
                 1483 
               
               
                   occidentale  (Cashew) 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Anacardium 
                 
                   Ana o  2 
                 Yes 
                 Legumin-like protein 
                 AAN76862 
                 Q8GZP6 
                 1484 
               
               
                   occidentale  (Cashew) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Anacardium 
                 
                   Ana o  3 
                 Yes 
                 2s albumin 
                 AAL91665 
                 Q8H2B8 
                 1485 
               
               
                   occidentale  (Cashew) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Apium graveolens 
                 
                 
                   Api g 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA88831 
                 P49372 
                 1486 
               
               
                 (Celery) 
                 1.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member, isoallergen 
                   
                   
                   
               
               
                   
                   
                   
                 1 
                   
                   
                   
               
               
                 
                   Apium graveolens 
                 
                 
                   Api g 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA99992 
                 P92918 
                 1487 
               
               
                 (Celery) 
                 1.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member, isoallergen 
                   
                   
                   
               
               
                   
                   
                   
                 2 
                   
                   
                   
               
               
                 
                   Apium graveolens 
                 
                   Api g  2 
                 Yes 
                 Non-specific lipid-transfer 
                 ACV04796 
                 E6Y8S8 
                 1488 
               
               
                 (Celery) 
                   
                   
                 protein, type 1 
                   
                   
                   
               
               
                 
                   Apium graveolens 
                 
                   Api g  3 
                 Yes 
                 Chlorophyll a-b binding 
                 CAA99993 
                 P92919 
                 1489 
               
               
                 (Celery) 
                   
                   
                 protein, chloroplast 
                   
                   
                   
               
               
                 
                   Apium graveolens 
                 
                   Api g  4 
                 Yes 
                 Profilin 
                 AAD29409 
                 Q9XF37 
                 1490 
               
               
                 (Celery) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Apium graveolens 
                 
                   Api g  5 
                 Yes 
                 FAD-containing oxidase 
                 P81943 
                 P81943 
                 1491 
               
               
                 (Celery) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Apium graveolens 
                 
                   Api g  6 
                 Yes 
                 Non-specific lipid transfer 
                 P86809 
                 P86809 
                 1492 
               
               
                 (Celery) 
                   
                   
                 protein type 2 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  1 
                 Yes 
                 Cupin (Vicillin-type, 7S 
                 AAB00861 
                 P43238 
                 1493 
               
               
                 (Peanut, groundnut) 
                   
                   
                 globulin) 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  2 
                 Yes 
                 Conglutin-7 (2S albumin), 
                 AAK96887 
                 Q6PSU2 
                 1494 
               
               
                 (Peanut, groundnut) 
                   
                   
                 isoform 1 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Cupin (Legumin-type, 11S 
                 AAC63045 
                 O82580 
                 1495 
               
               
                 (Peanut, groundnut) 
                 3.0101 
                   
                 globulin, Glycinin) 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Cupin (Legumin-type, 11S 
                 AAD47382 
                 Q9SQH7 
                 1496 
               
               
                 (Peanut, groundnut) 
                 3.0201 
                   
                 globulin, Glycinin) 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  5 
                 Yes 
                 Profilin 
                 AAD55587 
                 Q9SQI9 
                 1497 
               
               
                 (Peanut, groundnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  6 
                 Yes 
                 Conglutin (2S albumin) 
                 AAD56337 
                 Q647G9 
                 1498 
               
               
                 (Peanut, groundnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Conglutin (2S albumin) 
                 AAD56719 
                 Q9SQH1 
                 1499 
               
               
                 (Peanut, groundnut) 
                 7.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Conglutin (2S albumin) 
                 ABW17159 
                 B4XID4 
                 1500 
               
               
                 (Peanut, groundnut) 
                 7.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Pathogenesis-related 
                 AAQ91847 
                 Q6VT83 
                 1501 
               
               
                 (Peanut, groundnut) 
                 8.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Pathogenesis-related 
                 ABP97433 
                 B0YIU5 
                 1502 
               
               
                 (Peanut, groundnut) 
                 8.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Nonspecific lipid-transfer 
                 ABX56711 
                 B6CEX8 
                 1503 
               
               
                 (Peanut, groundnut) 
                 9.0101 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Nonspecific lipid-transfer 
                 ABX75045 
                 B6CG41 
                 1504 
               
               
                 (Peanut, groundnut) 
                 9.0201 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 16 kDa oleosin-1 
                 AAU21499 
                 Q647G5 
                 1505 
               
               
                 (Peanut, groundnut) 
                 10.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 16 kDa oleosin-2 
                 AAU21500 
                 Q647G4 
                 1506 
               
               
                 (Peanut, groundnut) 
                 10.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 14 kDa oleosin-1 
                 AAZ20276 
                 Q45W87 
                 1507 
               
               
                 (Peanut, groundnut) 
                 11.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 14 kDa oleosin-2 
                 AAZ20277 
                 Q45W86 
                 1508 
               
               
                 (Peanut, groundnut) 
                 11.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  12 
                 Yes 
                 Defensin 
                 / 
                 / 
                 / 
               
               
                 (Peanut, groundnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  13 
                 Yes 
                 Defensin 
                 / 
                 / 
                 / 
               
               
                 (Peanut, groundnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Oleosin, variant A 
                 AAK13449 
                 Q9AXI1 
                 1509 
               
               
                 (Peanut, groundnut) 
                 14.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Oleosin, variant B 
                 AAK13450 
                 Q9AXI0 
                 1510 
               
               
                 (Peanut, groundnut) 
                 14.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                 
                   Ara h 
                 
                 Yes 
                 Oleosin 
                 AAT11925 
                 Q6J1J8 
                 1511 
               
               
                 (Peanut, groundnut) 
                 14.0103 
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  15 
                 Yes 
                 Oleosin 
                 AAU21501 
                 Q647G3 
                 1512 
               
               
                 (Peanut, groundnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  16 
                 Yes 
                 non-specific Lipid Transfer 
                 / 
                 / 
                 / 
               
               
                 (Peanut, groundnut) 
                   
                   
                 Protein 2 
                   
                   
                   
               
               
                 
                   Arachis hypogaea 
                 
                   Ara h  17 
                 Yes 
                 non-specific Lipid Transfer 
                 / 
                 / 
                 / 
               
               
                 (Peanut, groundnut) 
                   
                   
                 Protein 1 
                   
                   
                   
               
               
                   Artemisia annua  (Sweet 
                   Art an  7 
                 No 
                 Galactose oxidase 
                 / 
                 / 
                 / 
               
               
                 Wormwood) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                   Art v  1 
                 No 
                 Defensin-like protein 
                 AAO24900 
                 Q84ZX5 
                 1513 
               
               
                 (Mugwort, wormwood) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                   Art v  2 
                 No 
                 Pathogenesis-related 
                 CAK50834 
                 A6GVD5 
                 1514 
               
               
                 (Mugwort, wormwood) 
                   
                   
                 protein PR-1 
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                 
                   Art v 
                 
                 No 
                 Nonspecific lipid transfer 
                 P0C088 
                 P0C088 
                 1515 
               
               
                 (Mugwort, wormwood) 
                 3.0101 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                 
                   Art v 
                 
                 No 
                 Nonspecific lipid transfer 
                 ACE07186 
                 C4MGG9 
                 1516 
               
               
                 (Mugwort, wormwood) 
                 3.0201 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                 
                   Art v 
                 
                 No 
                 Nonspecific lipid transfer 
                 ACE07187 
                 C4MGH0 
                 1517 
               
               
                 (Mugwort, wormwood) 
                 3.0202 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                 
                   Art v 
                 
                 No 
                 Nonspecific lipid transfer 
                 ACE07188 
                 C4MGH1 
                 1518 
               
               
                 (Mugwort, wormwood) 
                 3.0301 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                 
                   Art v 
                 
                 No 
                 Profilin-1 
                 CAD12861 
                 Q8H2C9 
                 1519 
               
               
                 (Mugwort, wormwood) 
                 4.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                 
                   Art v 
                 
                 No 
                 Profilin-2 
                 CAD12862 
                 Q8H2C8 
                 1520 
               
               
                 (Mugwort, wormwood) 
                 4.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                   Art v  5 
                 No 
                 Polcalcin 
                 AAX85389 
                 A0PJ17 
                 1521 
               
               
                 (Mugwort, wormwood) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Artemisia vulgaris 
                 
                   Art v  6 
                 No 
                 Pectate lyase 
                 AAX85388 
                 A0PJ16 
                 1522 
               
               
                 (Mugwort, wormwood) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Bertholletia excelsa 
                 
                   Ber e  1 
                 Yes 
                 2S sulfur-rich seed storage 
                 AAA33010 
                 P04403 
                 1523 
               
               
                 (Brazil nut) 
                   
                   
                 albumin 
                   
                   
                   
               
               
                 
                   Bertholletia excelsa 
                 
                   Ber e  2 
                 Yes 
                 11S globulin seed storage 
                 AAO38859 
                 Q84ND2 
                 1524 
               
               
                 (Brazil nut) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Beta vulgaris  (Sugar 
                   Beta v  1 
                 No 
                 Che a 1/Ole e 1 homolgue 
                 P85983 
                 P85983 
                 1525 
               
               
                 beet) 
                   
                   
                   
                   
                   
                   
               
               
                   Beta vulgaris  (Sugar 
                   Beta v  2 
                 No 
                 Profilin, pollen 
                 P85984 
                 P85984 
                 1526 
               
               
                 beet) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA33887 
                 P15494 
                 1527 
               
               
                 ( Betula pendula ) 
                 1.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-A 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54482 
                 P43177 
                 1528 
               
               
                 ( Betula pendula ) 
                 1.0102 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-D/H 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54483 
                 P43178 
                 1529 
               
               
                 ( Betula pendula ) 
                 1.0103 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-E 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54484 
                 P43179 
                 1530 
               
               
                 ( Betula pendula ) 
                 1.0104 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-F/I 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54485 
                 P43180 
                 1531 
               
               
                 ( Betula pendula ) 
                 1.0105 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-G 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54487 
                 P43183 
                 1532 
               
               
                 ( Betula pendula ) 
                 1.0106 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-J 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54489 
                 P43185 
                 1533 
               
               
                 ( Betula pendula ) 
                 1.0107 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-L 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAB02155 
                 Q96365 
                 1534 
               
               
                 ( Betula pendula ) 
                 1.0108 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAB02156 
                 Q96366 
                 1535 
               
               
                 ( Betula pendula ) 
                 1.0109 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAB02157 
                 Q96367 
                 1536 
               
               
                 ( Betula pendula ) 
                 1.0110 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAB02158 
                 Q96368 
                 1537 
               
               
                 ( Betula pendula ) 
                 1.0111 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAB02159 
                 P15494 
                 1538 
               
               
                 ( Betula pendula ) 
                 1.0112 
                   
                 protein, PR-10,  Bet v  1 
                   
                 (variant 
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-A 
                   
                 F63L) 
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAB02160 
                 Q96370 
                 1539 
               
               
                 ( Betula pendula ) 
                 1.0113 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAB02161 
                 Q96371 
                 1540 
               
               
                 ( Betula pendula ) 
                 1.0114 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAA96547 
                 Q39431 
                 1541 
               
               
                 ( Betula pendula ) 
                 1.0115 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAA04827.1 
                 O23748 
                 1542 
               
               
                 ( Betula pendula ) 
                 1.0116 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen), isoform 
                 CAA07323.1 
                 Q9SCI0 
                 1543 
               
               
                 ( Betula pendula ) 
                 1.0117 
                   
                 at37 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen), isoform 
                 CAA07329.1 
                 Q9SCH6 
                 1544 
               
               
                 ( Betula pendula ) 
                 1.0118 
                   
                 at5 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Major allergen  Bet v  1.01E 
                 ABC41615.1 
                 Q0QLS9 
                 1545 
               
               
                 ( Betula pendula ) 
                 1.0119 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54421 
                 P45431 
                 1546 
               
               
                 ( Betula pendula ) 
                 1.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-B 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54481 
                 P43176 
                 1547 
               
               
                 ( Betula pendula ) 
                 1.0202 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-C 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA54488 
                 P43184 
                 1548 
               
               
                 ( Betula pendula ) 
                 1.0203 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-K 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Pathogenesis-related 
                 CAA57550 
                 P43186 
                 1549 
               
               
                 ( Betula pendula ) 
                 1.0204 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                 family member 1-M/N 
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAA96540.1 
                 Q39427 
                 1550 
               
               
                 ( Betula pendula ) 
                 1.0205 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 / (pollen allergen) 
                 CAA04828.1 
                 O23749 
                 1551 
               
               
                 ( Betula pendula ) 
                 1.0206 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 Major allergen  Bet v  1.02C 
                 ACF75030.1 
                 Q0QLV2 
                 1552 
               
               
                 ( Betula pendula ) 
                 1.0207 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 1 Sc-3 protein 
                 CAA54696.1 
                 Q39415 
                 1553 
               
               
                 ( Betula pendula ) 
                 1.0301 
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                   Bet v  2 
                 No 
                 Profilin 
                 AAA16522 
                 P25816 
                 1554 
               
               
                 ( Betula pendula ) 
                   
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                   Bet v  3 
                 No 
                 Polcalcin-like protein (4 
                 CAA55854 
                 P43187 
                 1555 
               
               
                 ( Betula pendula ) 
                   
                   
                 EF-hand) 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                   Bet v  4 
                 No 
                 Polcalcin 
                 CAA60628 
                 Q39419 
                 1556 
               
               
                 ( Betula pendula ) 
                   
                   
                   
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 PhenylCoumaran benzylic 
                 AAC05116 
                 O65002 
                 1557 
               
               
                 ( Betula pendula ) 
                 6.0101 
                   
                 ether reductase 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                 
                   Bet v 
                 
                 No 
                 PhenylCoumaran benzylic 
                 AAG22740 
                 Q9FUW6 
                 1558 
               
               
                 ( Betula pendula ) 
                 6.0102 
                   
                 ether reductase 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Betula verrucosa 
                 
                   Bet v  7 
                 No 
                 Cyclophilin (Peptidyl- 
                 CAC84116 
                 P81531 
                 1559 
               
               
                 ( Betula pendula ) 
                   
                   
                 prolyl cis-trans isomerase) 
                   
                   
                   
               
               
                 (European white birch) 
                   
                   
                   
                   
                   
                   
               
               
                   Brassica juncea  (Indian 
                   Bra j  1 
                 Yes 
                 2S albumin seed storage 
                 P80207 
                 P80207 
                 1560 
               
               
                 or oriental mustard) 
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   Brassica napus 
                 
                   Bra n  1 
                 Yes 
                 2S albumin seed storage 
                 P80208 
                 P80208 
                 1561 
               
               
                 (Rapeseed) 
                   
                   
                 protein (Napin-3) 
                   
                   
                   
               
               
                 
                   Brassica oleracea 
                 
                   Bra o  3 
                 Yes 
                 non-specific lipid transfer 
                 / 
                 / 
                 / 
               
               
                 (Cabbage and others) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                   Brassica rapa  (Field 
                   Bra r  1 
                 Yes 
                 2S albumin 
                 CAA46782 
                 Q42473 
                 1562 
               
               
                 mustard or Turnip) 
                   
                   
                   
                   
                   
                   
               
               
                   Brassica rapa  (Field 
                   Bra r  2 
                 Yes 
                 Prohevein homologue 
                 / 
                 P81729 
                 1563 
               
               
                 mustard or Turnip) 
                   
                   
                 (Chitin-binding allergen) 
                   
                   
                   
               
               
                   Brassica rapa  (Field 
                   Bra r  5 
                 Yes 
                 Polcalcin 
                 BAA09634 
                 P69197 
                 1564 
               
               
                 mustard or Turnip) 
                   
                   
                   
                   
                   
                   
               
               
                   Cannabis sativa  (Indian 
                   Can s  3 
                 No 
                 Non-specific lipid transfer 
                 CCK33472 
                 W0U0V5 
                 1565 
               
               
                 hemp) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Capsicum annuum 
                 
                   Cap a  1 
                 Yes 
                 Osmotin-like protein 
                 CAC34055 
                 Q9ARG0 
                 1566 
               
               
                 (Chilli or bell pepper) 
                   
                   
                 (thaumatin-like protein) 
                   
                   
                   
               
               
                 
                   Capsicum annuum 
                 
                   Cap a  2 
                 Yes 
                 Profilin 
                 CAD10376 
                 Q93YI9 
                 1567 
               
               
                 (Chilli or bell pepper) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Carpinus betulus 
                 
                 
                   Car b 
                 
                 No 
                 Pathogenesis-related 
                 CAA47366 
                 P38949 
                 1568 
               
               
                 (Hornbeam) 
                 1.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 1A and 1B 
                   
                   
                   
               
               
                 
                   Carpinus betulus 
                 
                 
                   Car b 
                 
                 No 
                 Pathogenesis-related 
                 CAA47357 
                 P38949 
                 1568 
               
               
                 (Hornbeam) 
                 1.0102 
                   
                 protein, PR-10,  Bet v  1 
                   
                 (variant) 
                   
               
               
                   
                   
                   
                 family member 1A and 1B 
                   
                   
                   
               
               
                 
                   Carya illinoinensis 
                 
                   Car i  1 
                 Yes 
                 2S albumin seed storage 
                 AAO32314 
                 Q84XA9 
                 1569 
               
               
                 (Pecan) 
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   Carya illinoinensis 
                 
                   Car i  2 
                 Yes 
                 Vicilin-like protein 
                 ABV49590 
                 B3STU4 
                 1570 
               
               
                 (Pecan) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Carya illinoinensis 
                 
                   Car i  4 
                 Yes 
                 Legumin seed storage 
                 ABW86978 
                 B5KVH4 
                 1571 
               
               
                 (Pecan) 
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   Castanea sativa 
                 
                   Cas s  1 
                 No 
                 Pathogenesis-related 
                 ACJ23861 
                 B7TWE3 
                 1572 
               
               
                 (Chestnut) 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Castanea sativa 
                 
                   Cas s  5 
                 Yes 
                 Chitinase 
                 AAB01895 
                 Q42428 
                 1573 
               
               
                 (Chestnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Castanea sativa 
                 
                   Cas s  8 
                 Yes 
                 Non-specific lipid transfer 
                 / 
                 / 
                 / 
               
               
                 (Chestnut) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Castanea sativa 
                 
                   Cas s  9 
                 Yes 
                 Cytosolic class I small heat 
                 CAE46905 
                 Q9ZS24 
                 1574 
               
               
                 (Chestnut) 
                   
                   
                 shock protein 
                   
                   
                   
               
               
                 
                   Catharanthus roseus 
                 
                   Cat r  1 
                 No 
                 Cyclophilin 9Peptidyl- 
                 CAA59468 
                 I3QBM4 
                 1575 
               
               
                 (Rosy periwinkle) 
                   
                   
                 prolyl cis-trans isomerase) 
                   
                   
                   
               
               
                 
                   Chenopodium album 
                 
                   Che a  1 
                 No 
                 Ole e 1 homologue 
                 AAL07319 
                 Q8LGR0 
                 1576 
               
               
                 (Lambsquarters) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Chenopodium album 
                 
                   Che a  2 
                 No 
                 Profilin 
                 AAL92870 
                 Q84V37 
                 1577 
               
               
                 (Lambsquarters) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Chenopodium album 
                 
                   Che a  3 
                 No 
                 Polcalcin 
                 AAL92871 
                 Q84V36 
                 1578 
               
               
                 (Lambsquarters) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Citrullus lanatus 
                 
                   Citr l  2 
                 Yes 
                 Profilin 
                 AAU43733 
                 Q5XWE1 
                 1579 
               
               
                 (watermellon) 
                   
                   
                   
                   
                   
                   
               
               
                   Citrus limon  (Lemon) 
                   Cit l  3 
                 Yes 
                 Non-specific lipid-transfer 
                 P84160 
                 P84160 
                 1580 
               
               
                   
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   Citrus reticulata 
                 
                   Cit r  3 
                 Yes 
                 Non-specific lipid transfer 
                 P84161 
                 P84161 
                 1581 
               
               
                 (Tangerine) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                   Citrus sinensis  (Sweet 
                   Cit s  1 
                 Yes 
                 Germin-like protein 
                 P84159 
                 P84159 
                 1582 
               
               
                 orange) 
                   
                   
                   
                   
                   
                   
               
               
                   Citrus sinensis  (Sweet 
                   Cit s  2 
                 Yes 
                 Profilin 
                 CAI23765 
                 P84177 
                 1583 
               
               
                 orange) 
                   
                   
                   
                   
                   
                   
               
               
                   Citrus sinensis  (Sweet 
                   Cit s  3 
                 Yes 
                 Non-specific lipid-transfer 
                 CAH03799 
                 Q6EV47 
                 1584 
               
               
                 orange) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                   Citrus sinensis  (Sweet 
                   Cit s  7 
                 Yes 
                 Gibberellin regulated 
                 / 
                 / 
                 / 
               
               
                 orange) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Coffea arabica  (Arabian 
                   Cof a  1 
                 No 
                 Class III chitinase 
                 ADH10372 
                 D7REL9 
                 1585 
               
               
                 coffee) 
                   
                   
                   
                   
                   
                   
               
               
                   Coffea arabica  (Arabian 
                   Cof a  2 
                 No 
                 Metallothionein type 2 
                 AGL34967 
                 AGL34967 
                 1586 
               
               
                 coffee) 
                   
                   
                   
                   
                   
                   
               
               
                   Coffea arabica  (Arabian 
                   Cof a  3 
                 No 
                 Metallothionein type 3 
                 AGL34968 
                 R4MUV4 
                 1587 
               
               
                 coffee) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA50327 
                 Q08407 
                 1588 
               
               
                 (Hazelnut) 
                 1.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member; isoform 5, 
                   
                   
                   
               
               
                   
                   
                   
                 6, 11 and 16 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA96548 
                 Q39453 
                 1589 
               
               
                 (Hazelnut) 
                 1.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA96549 
                 Q39454 
                 1590 
               
               
                 (Hazelnut) 
                 1.0301 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD48405 
                 Q9SWR4 
                 1591 
               
               
                 (Hazelnut) 
                 1.0401 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Pathogenesis-related 
                 AAG40329 
                 Q9FPK4 
                 1592 
               
               
                 (Hazelnut) 
                 1.0402 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Pathogenesis-related 
                 AAG40330 
                 Q9FPK3 
                 1593 
               
               
                 (Hazelnut) 
                 1.0403 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Pathogenesis-related 
                 AAG40331 
                 Q9FPK2 
                 1594 
               
               
                 (Hazelnut) 
                 1.0404 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Profilin 
                 AAK01235 
                 Q9AXH5 
                 1595 
               
               
                 (Hazelnut) 
                 2.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                 
                   Cor a 
                 
                 Yes 
                 Profilin 
                 AAK01236 
                 Q9AXH4 
                 1596 
               
               
                 (Hazelnut) 
                 2.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                   Cor a  6 
                 No 
                 Isoflavone reductase 
                 / 
                 / 
                 / 
               
               
                 (Hazelnut) 
                   
                   
                 homologue 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                   Cor a  8 
                 Yes 
                 Non-specific lipid transfer 
                 AAK28533 
                 Q9ATH2 
                 1597 
               
               
                 (Hazelnut) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                   Cor a  9 
                 Yes 
                 11S seed storage globulin 
                 AAL73404 
                 Q8W1C2 
                 1598 
               
               
                 (Hazelnut) 
                   
                   
                 (legumin-like) 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                   Cor a  10 
                 No 
                 Luminal binding protein 
                 CAC14168 
                 Q9FSY7 
                 1599 
               
               
                 (Hazelnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                   Cor a  11 
                 Yes 
                 7S seed storage globulin 
                 AAL86739 
                 Q8S4P9 
                 1600 
               
               
                 (Hazelnut) 
                   
                   
                 (vicilin-like) 
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                   Cor a  12 
                 Yes 
                 17 kDa oelosin 
                 AAO67349 
                 Q84T21 
                 1601 
               
               
                 (Hazelnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                   Cor a  13 
                 Yes 
                 14-16 kDa oleosin 
                 AAO65960 
                 Q84T91 
                 1602 
               
               
                 (Hazelnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Corylus avellana 
                 
                   Cor a  14 
                 Yes 
                 2S albumin 
                 ACO56333 
                 D0PWG2 
                 1603 
               
               
                 (Hazelnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cucumis melo 
                 
                   Cuc m  1 
                 Yes 
                 Alkaline serine protease 
                 BAA06905 
                 Q39547 
                 1604 
               
               
                 (Muskmelon) 
                   
                   
                 (cucumisin) 
                   
                   
                   
               
               
                 
                   Cucumis melo 
                 
                   Cuc m  2 
                 Yes 
                 Profilin 
                 AAW69549 
                 Q5FX67 
                 1605 
               
               
                 (Muskmelon) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cucumis melo 
                 
                   Cuc m  3 
                 Yes 
                 Pathogenesis-related 
                 P83834 
                 P83834 
                 1606 
               
               
                 (Muskmelon) 
                   
                   
                 protein PR-1 
                   
                   
                   
               
               
                   Daucus carota  (Carrot) 
                 
                   Dau c 
                 
                 Yes 
                 Pathogenesis-related 
                 AAB01092 
                 O04298 
                 1607 
               
               
                   
                 1.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Daucus carota  (Carrot) 
                 
                   Dau c 
                 
                 Yes 
                 Pathogenesis-related 
                 AAL76932 
                 Q8SAE7 
                 1608 
               
               
                   
                 1.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Daucus carota  (Carrot) 
                 
                   Dau c 
                 
                 Yes 
                 PRP-like protein 
                 ADL32660 
                 D9ZHN9 
                 1609 
               
               
                   
                 1.0301 
                   
                   
                   
                   
                   
               
               
                   Daucus carota  (Carrot) 
                   Dau c  4 
                 Yes 
                 Profilin 
                 AAL76933 
                 Q8SAE6 
                 1610 
               
               
                   Daucus carota  (Carrot) 
                   Dau c  5 
                 Yes 
                 Isoflavone reductase-like 
                 AEY79728 
                 H2DF86 
                 1611 
               
               
                   
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   Fagopyrum esculentum 
                 
                   Fag e  2 
                 Yes 
                 2S albumin 
                 ABC18306 
                 Q2PS07 
                 1612 
               
               
                 (Common buckwheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Fagopyrum esculentum 
                 
                   Fag e  3 
                 Yes 
                 Vicilin 
                 ABQ10638 
                 A5HIX6 
                 1613 
               
               
                 (Common buckwheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Fagopyrum esculentum 
                 
                 
                   Fag e 
                 
                 Yes 
                 Antimicrobial Peptide 
                 / 
                 P0DKH7 
                 1614 
               
               
                 (Common buckwheat) 
                 4.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Fagopyrum esculentum 
                 
                 
                   Fag e 
                 
                 Yes 
                 Antimicrobial Peptide 
                 / 
                 P0DKH8 
                 1615 
               
               
                 (Common buckwheat) 
                 4.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Fagopyrum esculentum 
                 
                   Fag e  5 
                 Yes 
                 Vicilin-like protein 
                 AY536051.1 
                 Q6QJL1 
                 1616 
               
               
                 (Common buckwheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Fagopyrum tataricum 
                 
                   Fag t  2 
                 Yes 
                 2S albumin 
                 ADW27428 
                 E9NX73 
                 1617 
               
               
                 (Tartarian buckwheat) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Fagus sylvatica 
                 
                   Fag s  1 
                 No 
                 Pathogenesis-related 
                 ACJ23864 
                 B7TWE6 
                 1618 
               
               
                 (European beech) 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Fragaria ananassa 
                 
                   Fra a  1 
                 Yes 
                 Pathogenesis-related 
                 CAJ29538.1 
                 Q3T923 
                 1619 
               
               
                 (Strawberry) 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Fragaria ananassa 
                 
                 
                   Fra a 
                 
                 Yes 
                 Non-specific lipid transfer 
                 CAC86258 
                 Q8VX12 
                 1620 
               
               
                 (Strawberry) 
                 3.0101 
                   
                 protein type 1 (ltp6) 
                   
                   
                   
               
               
                 
                   Fragaria ananassa 
                 
                 
                   Fra a 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AAY83342 
                 Q4PLT9 
                 1621 
               
               
                 (Strawberry) 
                 3.0102 
                   
                 protein type 1 (ltp2) 
                   
                   
                   
               
               
                 
                   Fragaria ananassa 
                 
                 
                   Fra a 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AAY83341 
                 Q4PLU0 
                 1622 
               
               
                 (Strawberry) 
                 3.0201 
                   
                 protein type 1 (ltp1) 
                   
                   
                   
               
               
                 
                   Fragaria ananassa 
                 
                 
                   Fra a 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AAY83345 
                 Q4PLT6 
                 1623 
               
               
                 (Strawberry) 
                 3.0202 
                   
                 protein type 1 (ltp5) 
                   
                   
                   
               
               
                 
                   Fragaria ananassa 
                 
                   Fra a  4 
                 Yes 
                 Profolin 
                 XP_004287490 
                 P0C0Y3 
                 1624 
               
               
                 (Strawberry) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Fraxinus excelsior 
                 
                 
                   Fra e 
                 
                 No 
                 Ole e 1-like protein family 
                 AAQ08947 
                 Q7XAV4 
                 1625 
               
               
                 (Ash) 
                 1.0101 
                   
                 member 
                   
                   
                   
               
               
                 
                   Fraxinus excelsior 
                 
                 
                   Fra e 
                 
                 No 
                 Ole e 1-like protein family 
                 AAV74343 
                 Q5EXJ6 
                 1626 
               
               
                 (Ash) 
                 1.0102 
                   
                 member 
                   
                   
                   
               
               
                 
                   Fraxinus excelsior 
                 
                 
                   Fra e 
                 
                 No 
                 Ole e 1-like protein family 
                 AAQ83588 
                 Q6U740 
                 1627 
               
               
                 (Ash) 
                 1.0201 
                   
                 member 
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                   Gly m  1 
                 No 
                 Hydrophobic protein from 
                 Q9S8F3 
                 Q9S8F3 
                 1628 
               
               
                   
                   
                   
                 soybean 
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                   Gly m  2 
                 No 
                 Defensin 
                 / 
                 / 
                 / 
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 Profilin-1 
                 CAA11756 
                 O65809 
                 1629 
               
               
                   
                 3.0101 
                   
                   
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 Profilin-2 
                 CAA11755 
                 O65810 
                 1630 
               
               
                   
                 3.0102 
                   
                   
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                   Gly m  4 
                 Yes 
                 Stress-induced protein 
                 CAA42646 
                 P26987 
                 1631 
               
               
                   
                   
                   
                 SAM22 
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 alpha subunit of Beta- 
                 BAA23360 
                 O22120 
                 1632 
               
               
                   
                 5.0101 
                   
                 conglycinin (vicilin, 7S 
                   
                   
                   
               
               
                   
                   
                   
                 globulin) 
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 alpha subunit of Beta- 
                 BAA74452 
                 Q9FZP9 
                 1633 
               
               
                   
                 5.0201 
                   
                 conglycinin (vicilin, 7S 
                   
                   
                   
               
               
                   
                   
                   
                 globulin) 
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 beta chain of Beta- 
                 AAB23463 
                 P25974 
                 1634 
               
               
                   
                 5.0301 
                   
                 conglycinin 
                   
                 (variant 
                   
               
               
                   
                   
                   
                   
                   
                 F36L 
                   
               
               
                   
                   
                   
                   
                   
                 V51G 
                   
               
               
                   
                   
                   
                   
                   
                 F197L) 
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 Glycinin G1 (legumin, 11S 
                 BAC78522 
                 P04776 
                 1635 
               
               
                   
                 6.0101 
                   
                 globulin) 
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 Glycinin G2 
                 BAA00154 
                 P04405 
                 1636 
               
               
                   
                 6.0201 
                   
                   
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 Glycinin G3 
                 CAA33217 
                 P11828 
                 1637 
               
               
                   
                 6.0301 
                   
                   
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 Glycinin 
                 BAA74953 
                 Q9SB11 
                 1638 
               
               
                   
                 6.0401 
                   
                   
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                 
                   Gly m 
                 
                 Yes 
                 Glycinin A3B4 subunit 
                 BAB15802 
                 Q7GC77 
                 1639 
               
               
                   
                 6.0501 
                   
                   
                   
                   
                   
               
               
                   Glycine max  (Soybean) 
                   Gly m  7 
                 Yes 
                 Seed biotinylated protein 
                 ACS49840 
                 C6K8D1 
                 1640 
               
               
                   Glycine max  (Soybean) 
                   Gly m  8 
                 Yes 
                 2S albumin 
                 AAB71140 
                 P19594 
                 1641 
               
               
                 
                   Helianthus annuus 
                 
                   Hel a  1 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Sunflower) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Helianthus annuus 
                 
                   Hel a  2 
                 No 
                 Profilin 
                 CAA75506 
                 O81982 
                 1642 
               
               
                 (Sunflower) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Helianthus annuus 
                 
                   Hel a  3 
                 Yes 
                 Non-specific lipid transfer 
                 AAP47226 
                 Q7X9Q5 
                 1643 
               
               
                 (Sunflower) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  1 
                 No 
                 Rubber elongation factor 
                 CAA39880 
                 P15252 
                 1644 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  2 
                 No 
                 beta-1,3-glucanase, basic 
                 AAA87456 
                 P52407 
                 1645 
               
               
                 rubber tree (latex)) 
                   
                   
                 vacuolar isoform 
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  3 
                 No 
                 Small rubber particle 
                 AAC82355 
                 O82803 
                 1646 
               
               
                 rubber tree (latex)) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  4 
                 No 
                 Lecithinase homologue 
                 AAR98518 
                 Q6T4P0 
                 1647 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  5 
                 No 
                 / 
                 AAC49447 
                 Q39967 
                 1648 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  6 
                 No 
                 Hevein precursor 
                 AAA33357 
                 P02877 
                 1649 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  7 
                 No 
                 Patatin-like protein 
                 AAC27724 
                 O04008 
                 1650 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Profilin-1 
                 CAA75312 
                 O65812 
                 1651 
               
               
                 rubber tree (latex)) 
                 8.0101 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Profilin-2 
                 CAB51914 
                 Q9STB6 
                 1652 
               
               
                 rubber tree (latex)) 
                 8.0102 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Profilin-3 
                 AAF34341 
                 Q9M7N0 
                 1653 
               
               
                 rubber tree (latex)) 
                 8.0201 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Profilin-4 
                 AAF34342 
                 Q9M7M9 
                 1654 
               
               
                 rubber tree (latex)) 
                 8.0202 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Profilin-5 
                 AAF34343 
                 Q9M7M8 
                 1655 
               
               
                 rubber tree (latex)) 
                 8.0203 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Profilin-6 
                 CAB96215 
                 Q9LEI8 
                 1656 
               
               
                 rubber tree (latex)) 
                 8.0204 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  9 
                 No 
                 Enolase 
                 CAC00532 
                 Q9LEJ0 
                 1657 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Superoxide dismutase 
                 AAA16792 
                 P35017 
                 1658 
               
               
                 rubber tree (latex)) 
                 10.0101 
                   
                 (Mn), mitochondrial 
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Superoxide dismutase 
                 CAB53458 
                 Q9STB5 
                 1659 
               
               
                 rubber tree (latex)) 
                 10.0102 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Superoxide dismutase 
                 CAC13961 
                 Q9FSJ2 
                 1660 
               
               
                 rubber tree (latex)) 
                 10.0103 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Class I chitinase 
                 CAC42881 
                 Q949H3 
                 1661 
               
               
                 rubber tree (latex)) 
                 11.0101 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                 
                   Hev b 
                 
                 No 
                 Class I chitinase 
                 CAD24068 
                 Q8GUD7 
                 1662 
               
               
                 rubber tree (latex)) 
                 11.0102 
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  12 
                 No 
                 Non-specific lipid transfer 
                 AAL25839 
                 Q8RYA8 
                 1663 
               
               
                 rubber tree (latex)) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  13 
                 No 
                 Esterase 
                 AAP37470 
                 Q7Y1X1 
                 1664 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  14 
                 No 
                 Hevamine 
                 ADR82196 
                 E7BQV3 
                 1665 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                   Hevea brasiliensis  (Para 
                   Hev b  15 
                 No 
                 Serine protease inhibitor 
                 CCW27997 
                 W0USW9 
                 1666 
               
               
                 rubber tree (latex)) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Humulus japonicus 
                 
                   Hum j  1 
                 No 
                 / 
                 AAP94213 
                 Q7XBE3 
                 1667 
               
               
                 (Japanese hop) 
                   
                   
                   
                   
                   
                   
               
               
                   Juglans nigra  (Black 
                   Jug n  1 
                 Yes 
                 2S albumin seed storage 
                 AAM54365 
                 Q7Y1C2 
                 1668 
               
               
                 walnut) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Juglans nigra  (Black 
                   Jug n  2 
                 Yes 
                 Vicilin seed storage protein 
                 AAM54366 
                 Q7Y1C1 
                 1669 
               
               
                 walnut) 
                   
                   
                   
                   
                   
                   
               
               
                   Juglans nigra  (Black 
                   Jug n  4 
                 Yes 
                 Legumin 
                 / 
                 / 
                 / 
               
               
                 walnut) 
                   
                   
                   
                   
                   
                   
               
               
                   Juglans regia  (English 
                   Jug r  1 
                 Yes 
                 2S albumin seed storage 
                 AAB41308 
                 P93198 
                 1670 
               
               
                 walnut) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Juglans regia  (English 
                   Jug r  2 
                 Yes 
                 Vicilin seed storage protein 
                 AAF18269 
                 Q9SEW4 
                 1671 
               
               
                 walnut) 
                   
                   
                   
                   
                   
                   
               
               
                   Juglans regia  (English 
                   Jug r  3 
                 Yes 
                 Non-specific lipid transfer 
                 ACI47547 
                 C5H617 
                 1672 
               
               
                 walnut) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                   Juglans regia  (English 
                   Jug r  4 
                 Yes 
                 11S globulin seed storage 
                 AAW29810 
                 Q2TPW5 
                 1673 
               
               
                 walnut) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Juglans regia  (English 
                   Jug r  5 
                 Yes 
                 Pathogenesis Related 
                 APD76154.1 
                 / 
                 1674 
               
               
                 walnut) 
                   
                   
                 protein-10 
                   
                   
                   
               
               
                   Juglans regia  (English 
                   Jug r  6 
                 Yes 
                 vicilin-like cupin 
                 / 
                 / 
                 / 
               
               
                 walnut) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Kochia scoparia 
                 
                   Koc s  1 
                 No 
                 Ole e 1-like protein 
                 AKV72169 
                 A0A0K1SC44 
                 1675 
               
               
                 (Burning bush) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Kochia scoparia 
                 
                   Koc s  2 
                 No 
                 profilin 
                 AIV43661.1 
                 / 
                 1676 
               
               
                 (Burning bush) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Lactuca sativa 
                 
                   Lac s  1 
                 Yes 
                 Non-specific lipid transfer 
                 / 
                 / 
                 / 
               
               
                 (Cultivated lettuce) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Lens culinaris  (Lentil) 
                 
                   Len c 
                 
                 Yes 
                 Gamma-vicilin subunit 
                 CAD87730 
                 Q84UI1 
                 1677 
               
               
                   
                 1.0101 
                   
                   
                   
                   
                   
               
               
                   Lens culinaris  (Lentil) 
                 
                   Len c 
                 
                 Yes 
                 Gamma-vicilin subunit 
                 CAD87731 
                 Q84UI0 
                 1678 
               
               
                   
                 1.0102 
                   
                   
                   
                   
                   
               
               
                   Lens culinaris  (Lentil) 
                   Len c  2 
                 Yes 
                 Seed-specific biotinylated 
                 / 
                 / 
                 / 
               
               
                   
                   
                   
                 protein 
                   
                   
                   
               
               
                   Lens culinaris  (Lentil) 
                   Len c  3 
                 Yes 
                 Nonspecific lipid transfer 
                 AAX35807 
                 A0AT29 
                 1679 
               
               
                   
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Ligustrum vulgare 
                 
                   Lig v  1 
                 No 
                 Ole e 1-like protein family 
                 CAA54818 
                 O82015 
                 1680 
               
               
                 (Common privet) 
                   
                   
                 member 
                   
                   
                   
               
               
                 
                   Litchi chinensis 
                 
                   Lit c  1 
                 Yes 
                 Profilin 
                 AAL07320 
                 Q941H7 
                 1681 
               
               
                 (Lychee) 
                   
                   
                   
                   
                   
                   
               
               
                   Lupinus  ( albus  ) 
                   Lup a  5 
                 Yes 
                 Profilin 
                 / 
                 / 
                 / 
               
               
                 
                   Lupinus angustifolius 
                 
                   Lup an  1 
                 Yes 
                 Conglutin beta (7S seed 
                 ACB05815 
                 B8Q5G0 
                 1682 
               
               
                 (Narrow-leaved blue 
                   
                   
                 storage globulin, vicilin) 
                   
                   
                   
               
               
                 lupin) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA58646 
                 P43211 
                 1683 
               
               
                 (Apple) 
                 1.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26546 
                 Q9SYV2 
                 1684 
               
               
                 (Apple) 
                 1.0103 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26552 
                 Q9SYV5 
                 1685 
               
               
                 (Apple) 
                 1.0104 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26553 
                 Q9SYV6 
                 1686 
               
               
                 (Apple) 
                 1.0105 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26554 
                 Q9SYV7 
                 1687 
               
               
                 (Apple) 
                 1.0106 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26555 
                 Q9SYV8 
                 1688 
               
               
                 (Apple) 
                 1.0107 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD29671 
                 Q9SYW3 
                 1689 
               
               
                 (Apple) 
                 1.0108 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAK13029 
                 Q941P6 
                 1690 
               
               
                 (Apple) 
                 1.0109 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAB01362 
                 Q40280 
                 1691 
               
               
                 (Apple) 
                 1.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26545 
                 Q9S7M5 
                 1691 
               
               
                 (Apple) 
                 1.0202 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26547 
                 Q9SYV3 
                 1692 
               
               
                 (Apple) 
                 1.0203 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26548 
                 Q9SYV4 
                 1693 
               
               
                 (Apple) 
                 1.0204 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD26558 
                 Q9SYV9 
                 1694 
               
               
                 (Apple) 
                 1.0205 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAD13683 
                 Q40280 
                 1695 
               
               
                 (Apple) 
                 1.0206 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Ribonuclease-like PR-10a 
                 AAK13030 
                 Q941P5 
                 1696 
               
               
                 (Apple) 
                 1.0207 
                   
                   
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 CAD32318 
                 Q8L6K9 
                 1697 
               
               
                 (Apple) 
                 1.0208 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA96534 
                 Q43549 
                 1698 
               
               
                 (Apple) 
                 1.0301 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAK13027 
                 Q941P8 
                 1699 
               
               
                 (Apple) 
                 1.0302 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAK13028 
                 Q941P7 
                 1700 
               
               
                 (Apple) 
                 1.0303 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 AAO25113 
                 Q84LA7 
                 1701 
               
               
                 (Apple) 
                 1.0304 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA96535 
                 Q43550 
                 1702 
               
               
                 (Apple) 
                 1.0401 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA96536 
                 Q43551 
                 1703 
               
               
                 (Apple) 
                 1.0402 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Pathogenesis-related 
                 CAA96537 
                 Q43552 
                 1704 
               
               
                 (Apple) 
                 1.0403 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                   Mal d  2 
                 Yes 
                 Thaumatin-like protein 
                 AAC36740 
                 Q9FSG7 
                 1705 
               
               
                 (Apple) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AAT80637 
                 Q5J026 
                 1706 
               
               
                 (Apple) 
                 3.0101w 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AAT80649 
                 Q5J011 
                 1707 
               
               
                 (Apple) 
                 3.0102w 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AAT80656 
                 Q5J009 
                 1708 
               
               
                 (Apple) 
                 3.0201w 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AAT80664 
                 Q5IZZ6 
                 1709 
               
               
                 (Apple) 
                 3.0202w 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AAT80665 
                 Q5IZZ5 
                 1710 
               
               
                 (Apple) 
                 3.0203w 
                   
                 protein type 1 
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Profilin-3 
                 AAD29414 
                 Q9XF42 
                 1711 
               
               
                 (Apple) 
                 4.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Profilin (pf-3) 
                 CAD46561 
                 Q84RR5 
                 1712 
               
               
                 (Apple) 
                 4.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Profilin-2 
                 AAD29413 
                 Q9XF41 
                 1713 
               
               
                 (Apple) 
                 4.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Profilin (pf-2) 
                 CAD46560 
                 Q84RR6 
                 1714 
               
               
                 (Apple) 
                 4.0202 
                   
                   
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Profilin-1 
                 AAD29412 
                 Q9XF40 
                 1715 
               
               
                 (Apple) 
                 4.0301 
                   
                   
                   
                   
                   
               
               
                 
                   Malus domestica 
                 
                 
                   Mal d 
                 
                 Yes 
                 Profilin (pf-1) 
                 CAD46559 
                 Q84RR7 
                 1716 
               
               
                 (Apple) 
                 4.0302 
                   
                   
                   
                   
                   
               
               
                 
                   Manihot esculenta 
                 
                   Man e  5 
                 Yes 
                 Glutamic acid rich protein 
                 AEE98392 
                 M1E7Y0 
                 1717 
               
               
                 (Cassava, manioc) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Mercurialis annua 
                 
                   Mer a  1 
                 No 
                 Profilin 
                 CAA73720 
                 O49894 
                 1718 
               
               
                 (Annual mercury) 
                   
                   
                   
                   
                   
                   
               
               
                   Morus nigra  (Mulberry) 
                   Mor n  3 
                 Yes 
                 Non-specific lipid transfer 
                 P85894 
                 P85894 
                 1719 
               
               
                   
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                   Olea europaea  (Olive) 
                   Ole e  1 
                 No 
                 Common olive group 1 
                 AAB32652 
                 P19963 
                 1720 
               
               
                   Olea europaea  (Olive) 
                   Ole e  2 
                 No 
                 Profilin-1 
                 CAA73035 
                 O24169 
                 1721 
               
               
                   Olea europaea  (Olive) 
                   Ole e  3 
                 No 
                 Polcalcin 
                 AAD05375 
                 O81092 
                 1722 
               
               
                   Olea europaea  (Olive) 
                   Ole e  4 
                 No 
                 major pollen allergen 
                 P80741 
                 P80741 
                 1723 
               
               
                   Olea europaea  (Olive) 
                   Ole e  5 
                 No 
                 Superoxide dismutase [Cu—Zn] 
                 P80740 
                 P80740 
                 1724 
               
               
                   Olea europaea  (Olive) 
                   Ole e  6 
                 No 
                 major pollen allergen 
                 AAB66909 
                 O24172 
                 1725 
               
               
                   Olea europaea  (Olive) 
                   Ole e  7 
                 No 
                 putative non-specific lipid 
                 P81430 
                 P81430 
                 1726 
               
               
                   
                   
                   
                 transfer protein 
                   
                   
                   
               
               
                   Olea europaea  (Olive) 
                   Ole e  8 
                 No 
                 Polcalcin-like protein (4 
                 AAF31151 
                 Q9M7R0 
                 1727 
               
               
                   
                   
                   
                 EF-hands) 
                   
                   
                   
               
               
                   Olea europaea  (Olive) 
                   Ole e  9 
                 No 
                 1 3-beta glucanase 
                 AAK58515 
                 Q94G86 
                 1728 
               
               
                   Olea europaea  (Olive) 
                   Ole e  10 
                 No 
                 X8 domain containing 
                 AAL92578 
                 Q84V39 
                 1729 
               
               
                   
                   
                   
                 protein 
                   
                   
                   
               
               
                   Olea europaea  (Olive) 
                   Ole e  11 
                 No 
                 Pectin methylesterase 
                 ACZ57582 
                 D8VPP5 
                 1730 
               
               
                 
                   Ostrya carpinifolia 
                 
                   Ost c  1 
                 No 
                 Pathogenesis-related 
                 ADK39021 
                 E2GL17 
                 1731 
               
               
                 (European 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                 hophornbeam) 
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Probable non-specific 
                 CAA54587 
                 P43217 
                 1732 
               
               
                 (Pellitory-of-the-Wall) 
                 1.0101 
                   
                 lipid-transfer protein 
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Probable non-specific 
                 CAA65123 
                 O04404 
                 1733 
               
               
                 (Pellitory-of-the-Wall) 
                 1.0102 
                   
                 lipid-transfer protein 1 
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Probable non-specific 
                 CAI94601 
                 Q1JTN5 
                 1734 
               
               
                 (Pellitory-of-the-Wall) 
                 1.0103 
                   
                 lipid-transfer protein 
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Probable non-specific 
                 CAA59370 
                 Q40905 
                 1735 
               
               
                 (Pellitory-of-the-Wall) 
                 1.0201 
                   
                 lipid-transfer protein 1 
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Phospholipid transfer 
                 CAA65121 
                 P55958 
                 1736 
               
               
                 (Pellitory-of-the-Wall) 
                 2.0101 
                   
                 protein 
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Phospholipid transfer 
                 CAA65122 
                 O04403 
                 1737 
               
               
                 (Pellitory-of-the-Wall) 
                 2.0102 
                   
                 protein 
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Profilin-1 
                 CAB44256 
                 Q9XG85 
                 1738 
               
               
                 (Pellitory-of-the-Wall) 
                 3.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Profilin-2 
                 CAB44257 
                 Q9T0M8 
                 1739 
               
               
                 (Pellitory-of-the-Wall) 
                 3.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                 
                   Par j 
                 
                 No 
                 Profilin 
                 CCP19647 
                 L8BTD8 
                 1740 
               
               
                 (Pellitory-of-the-Wall) 
                 3.0201 
                   
                   
                   
                   
                   
               
               
                 
                   Parietaria judaica 
                 
                   Par j  4 
                 No 
                 Polcalcin 
                 CAP05019 
                 B5QST3 
                 1741 
               
               
                 (Pellitory-of-the-Wall) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   ietaria officinalis 
                 
                   Par o  1 
                 No 
                 Phospholipid transfer 
                 / 
                 / 
                 / 
               
               
                 (Pellitory) 
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   Parthenium 
                 
                   Par h  1 
                 No 
                 pollen defensin-like 
                 AKF12278 
                 A0A0X9C7K4 
                 1742 
               
               
                 
                   hysterophorus 
                 
                   
                   
                 protein 
                   
                   
                   
               
               
                 (Feverfew) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Persea americana 
                 
                   Pers a  1 
                 Yes 
                 Endochitinase 
                 CAB01591 
                 P93680 
                 1743 
               
               
                 (Avocado) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phaseolus vulgaris 
                 
                 
                   Pha v 
                 
                 Yes 
                 non-specific lipid transfer 
                 ADC80502 
                 D3W146 
                 1744 
               
               
                 (Green bean, French 
                 3.0101 
                   
                 protein type 1 
                   
                   
                   
               
               
                 bean) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Phaseolus vulgaris 
                 
                 
                   Pha v 
                 
                 Yes 
                 non-specific lipid transfer 
                 ADC80503 
                 D3W147 
                 1745 
               
               
                 (Green bean, French 
                 3.0201 
                   
                 protein type 1 
                   
                   
                   
               
               
                 bean) 
                   
                   
                   
                   
                   
                   
               
               
                   Pistacia vera  (Pistachio) 
                   Pis v  1 
                 Yes 
                 2S albumin 
                 ABG73108 
                 B7P072 
                 1746 
               
               
                   Pistacia vera  (Pistachio) 
                 
                   Pis v 
                 
                 Yes 
                 11S globulin subunit 
                 ABG73109 
                 B7P073 
                 1747 
               
               
                   
                 2.0101 
                   
                 presucor 
                   
                   
                   
               
               
                   Pistacia vera  (Pistachio) 
                 
                   Pis v 
                 
                 Yes 
                 11S globulin subunit 
                 ABG73110 
                 B7P074 
                 1748 
               
               
                   
                 2.0201 
                   
                 presucor 
                   
                   
                   
               
               
                   Pistacia vera  (Pistachio) 
                   Pis v  3 
                 Yes 
                 vicillin 
                 ABO36677 
                 B4X640 
                 1749 
               
               
                   Pistacia vera  (Pistachio) 
                   Pis v  4 
                 Yes 
                 manganese superoxide 
                 ABR29644 
                 B2BDZ8 
                 1750 
               
               
                   
                   
                   
                 dismutase 
                   
                   
                   
               
               
                   Pistacia vera  (Pistachio) 
                   Pis v  5 
                 Yes 
                 11S globulin subunit 
                 ACB55490 
                 B7SLJ1 
                 1751 
               
               
                   Pisum sativum  (Pea) 
                 
                   Pis s 
                 
                 Yes 
                 Vicilin 
                 CAF25232 
                 Q702P1 
                 1752 
               
               
                   
                 1.0101 
                   
                   
                   
                   
                   
               
               
                   Pisum sativum  (Pea) 
                 
                   Pis s 
                 
                 Yes 
                 Vicilin 
                 CAF25233 
                 Q702P0 
                 1753 
               
               
                   
                 1.0102 
                   
                   
                   
                   
                   
               
               
                   Pisum sativum  (Pea) 
                   Pis s  2 
                 Yes 
                 Convicilin 
                 CAB82855 
                 P13915 
                 1754 
               
               
                   Pisum sativum  (Pea) 
                   Pis s  3 
                 Yes 
                 Non-specific lipid-transfer 
                 AJG44053 
                 C0HJR7 
                 1755 
               
               
                   
                   
                   
                 protein 1 
                   
                   
                   
               
               
                 
                   Plantago lanceolata 
                 
                 
                   Pla l 
                 
                 No 
                 Ole e 1-related protein 
                 CAC41633 
                 P82242 
                 1756 
               
               
                 (English plantain) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Plantago lanceolata 
                 
                 
                   Pla l 
                 
                 No 
                 Ole e 1-related protein 
                 CAC41634 
                 P82242 
                 1756 
               
               
                 (English plantain) 
                 1.0102 
                   
                   
                   
                 (variant 
                   
               
               
                   
                   
                   
                   
                   
                 D58G) 
                   
               
               
                 
                   Plantago lanceolata 
                 
                 
                   Pla l 
                 
                 No 
                 Ole e 1-related protein 
                 CAC41635 
                 P82242 
                 1756 
               
               
                 (English plantain) 
                 1.0103 
                   
                   
                   
                 (variant 
                   
               
               
                   
                   
                   
                   
                   
                 D58G 
                   
               
               
                   
                   
                   
                   
                   
                 S82G) 
                   
               
               
                 
                   Plantago lanceolata 
                 
                   Pla l  2 
                 No 
                 Profilin 
                 / 
                 C0HJX6 
                 1757 
               
               
                 (English plantain) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Platanus acerifolia 
                 
                   Pla a  1 
                 No 
                 Putative invertase inhibitor 
                 CAD20556 
                 Q8GT41 
                 1758 
               
               
                 (London plane tree) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Platanus acerifolia 
                 
                   Pla a  2 
                 No 
                 Polygalacturonase 
                 CAE52833 
                 Q6H9K0 
                 1759 
               
               
                 (London plane tree) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Platanus acerifolia 
                 
                   Pla a  3 
                 No 
                 Non-specific lipid transfer 
                 / 
                 / 
                 / 
               
               
                 (London plane tree) 
                   
                   
                 protein 1 
                   
                   
                   
               
               
                 
                   Platanus orientalis 
                 
                   Pla or  1 
                 No 
                 Plant invertase/pectin 
                 ABY21305 
                 A9YUH4 
                 1760 
               
               
                 (Oriental plane) 
                   
                   
                 methylesterase inhibitor 
                   
                   
                   
               
               
                 
                   Platanus orientalis 
                 
                   Pla or  2 
                 No 
                 Polygalacturonase 
                 ABY21306 
                 A9YUH5 
                 1761 
               
               
                 (Oriental plane) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Platanus orientalis 
                 
                   Pla or  3 
                 No 
                 Non-specific lipid-transfer 
                 ABY21307 
                 A9YUH6 
                 1762 
               
               
                 (Oriental plane) 
                   
                   
                 protein 1 
                   
                   
                   
               
               
                 
                   Prosopis juliflora 
                 
                   Pro j  1 
                 No 
                 Ole e 1-like protein 
                 AKV72167.1 
                 / 
                 1763 
               
               
                 (Mesquite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Prosopis juliflora 
                 
                   Pro j  2 
                 No 
                 Profilin 
                 AHY24177 
                 A0A023W2L7 
                 1764 
               
               
                 (Mesquite) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Prunus armeniaca 
                 
                   Pru ar  1 
                 Yes 
                 Pathogenesis-related 
                 AAB97141 
                 O50001 
                 1765 
               
               
                 (Apricot) 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Prunus armeniaca 
                 
                   Pru ar  3 
                 Yes 
                 Non-specific lipid transfer 
                 P81651 
                 P81651 
                 1766 
               
               
                 (Apricot) 
                   
                   
                 protein 1 
                   
                   
                   
               
               
                   Prunus avium  (Sweet 
                 
                   Pru av 
                 
                 Yes 
                 Pathogenesis-related 
                 AAC02632 
                 O24248 
                 1767 
               
               
                 cherry) 
                 1.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Prunus avium  (Sweet 
                 
                   Pru av 
                 
                 Yes 
                 Pathogenesis-related 
                 AAS47035 
                 Q6QHU3 
                 1768 
               
               
                 cherry) 
                 1.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Prunus avium  (Sweet 
                 
                   Pru av 
                 
                 Yes 
                 Pathogenesis-related 
                 AAS47036 
                 Q6QHU2 
                 1769 
               
               
                 cherry) 
                 1.0202 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Prunus avium  (Sweet 
                 
                   Pru av 
                 
                 Yes 
                 Pathogenesis-related 
                 AAS47037 
                 Q6QHU1 
                 1770 
               
               
                 cherry) 
                 1.0203 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Prunus avium  (Sweet 
                   Pru av  2 
                 Yes 
                 Thaumatin-like protein 
                 AAB38064 
                 P50694 
                 1771 
               
               
                 cherry) 
                   
                   
                   
                   
                   
                   
               
               
                   Prunus avium  (Sweet 
                   Pru av  3 
                 Yes 
                 Non-specific lipid transfer 
                 AAF26449 
                 Q9M5X8 
                 1772 
               
               
                 cherry) 
                   
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                   Prunus avium  (Sweet 
                   Pru av  4 
                 Yes 
                 Profilin 
                 AAD29411 
                 Q9XF39 
                 1773 
               
               
                 cherry) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Prunus domestica 
                 
                   Pru d  3 
                 Yes 
                 Non-specific lipid transfer 
                 P82534 
                 P82534 
                 1774 
               
               
                 (European plum) 
                   
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                   Prunus dulcis  (Almond) 
                   Pru du  3 
                 Yes 
                 Non-specific lipid transfer 
                 ACN11576 
                 C0L0I5 
                 1775 
               
               
                   
                   
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                   Prunus dulcis  (Almond) 
                   Pru du  4 
                 Yes 
                 Profilin 
                 AAL91662 
                 Q8GSL5 
                 1776 
               
               
                   Prunus dulcis  (Almond) 
                   Pru du  5 
                 Yes 
                 60s acidic ribosomal prot. 
                 ABH03379 
                 Q8H2B9 
                 1777 
               
               
                   
                   
                   
                 P2 
                   
                   
                   
               
               
                   Prunus dulcis  (Almond) 
                 
                   Pru du 
                 
                 Yes 
                 Prunin-1 (Amandin, 11S 
                 ADN39440 
                 E3SH28 
                 1778 
               
               
                   
                 6.0101 
                   
                 globulin legumin-like 
                   
                   
                   
               
               
                   
                   
                   
                 protein) 
                   
                   
                   
               
               
                   Prunus dulcis  (Almond) 
                 
                   Pru du 
                 
                 Yes 
                 Prunin-2 
                 ADN39441 
                 E3SH29 
                 1779 
               
               
                   
                 6.0201 
                   
                   
                   
                   
                   
               
               
                   Prunus mume  (Japanese 
                   Pru m  7 
                 Yes 
                 gibberellin-regulated 
                 XP_016648029.1 
                 / 
                 1780 
               
               
                 apricot) 
                   
                   
                 protein 
                   
                   
                   
               
               
                   Prunus persica  (Peach) 
                   Pru p  1 
                 Yes 
                 Pathogenesis-related 
                 ABB78006 
                 Q2I6V8 
                 1781 
               
               
                   
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Prunus persica  (Peach) 
                 
                   Pru p 
                 
                 Yes 
                 Thaumatin-like protein 
                 ACE80959 
                 B6CQT7 
                 1782 
               
               
                   
                 2.0101 
                   
                   
                   
                   
                   
               
               
                   Prunus persica  (Peach) 
                 
                   Pru p 
                 
                 Yes 
                 Thaumatin-like protein 
                 ACE80957 
                 B6CQT5 
                 1783 
               
               
                   
                 2.0201 
                   
                   
                   
                   
                   
               
               
                   Prunus persica  (Peach) 
                 
                   Pru p 
                 
                 Yes 
                 Thaumatin-like protein 
                 ACE80955 
                 B6CQT3 
                 1784 
               
               
                   
                 2.0301 
                   
                   
                   
                   
                   
               
               
                   Prunus persica  (Peach) 
                 
                   Pru p 
                 
                 Yes 
                 Non-specific lipid transfer 
                 P81402 
                 P81402 
                 1785 
               
               
                   
                 3.0101 
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                   Prunus persica  (Peach) 
                 
                   Pru p 
                 
                 Yes 
                 Non-specific lipid transfer 
                 CAB96876 
                 Q9LED1 
                 1786 
               
               
                   
                 3.0102 
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                   Prunus persica  (Peach) 
                   Pru p  4 
                 Yes 
                 Profilin 
                 CAD37201 
                 Q8GT40 
                 1787 
               
               
                   Prunus persica  (Peach) 
                   Pru p  7 
                 Yes 
                 Gibberellin-regulated 
                 P86888 
                 P86888 
                 1788 
               
               
                   
                   
                   
                 protein 
                   
                   
                   
               
               
                 
                   Punica granatum 
                 
                 
                   Pun g 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AHB19227 
                 A0A059STC4 
                 1789 
               
               
                 (Pomegranate) 
                 1.0101 
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                 
                   Punica granatum 
                 
                 
                   Pun g 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AHB19226 
                 A0A059SSZ0 
                 1790 
               
               
                 (Pomegranate) 
                 1.0201 
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                 
                   Punica granatum 
                 
                 
                   Pun g 
                 
                 Yes 
                 Non-specific lipid transfer 
                 AHB19225 
                 A0A059ST23 
                 1791 
               
               
                 (Pomegranate) 
                 1.0301 
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                   Pyrus communis  (Pear) 
                   Pyr c  1 
                 Yes 
                 Pathogenesis-related 
                 AAC13315 
                 O65200 
                 1792 
               
               
                   
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Pyrus communis  (Pear) 
                   Pyr c  3 
                 Yes 
                 Nonspecific lipid-transfer 
                 AAF26451 
                 Q9M5X6 
                 1793 
               
               
                   
                   
                   
                 protein 1 
                   
                   
                   
               
               
                   Pyrus communis  (Pear) 
                   Pyr c  4 
                 Yes 
                 Profilin 
                 AAD29410 
                 Q9XF38 
                 1794 
               
               
                   Pyrus communis  (Pear) 
                   Pyr c  5 
                 Yes 
                 Isoflavone reductase 
                 AAC24001 
                 O81355 
                 1795 
               
               
                   
                   
                   
                 related protein, putative 
                   
                   
                   
               
               
                   
                   
                   
                 phenylcoumaran benzylic 
                   
                   
                   
               
               
                   
                   
                   
                 ether reductase 
                   
                   
                   
               
               
                   Quercus alba  (White 
                 
                   Que a 
                 
                 No 
                 Pathogenesis-related 
                 ABZ81045 
                 B6RQS1 
                 1796 
               
               
                 oak) 
                 1.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Quercus alba  (White 
                 
                   Que a 
                 
                 No 
                 Pathogenesis-related 
                 ABZ81046 
                 B6RQS2 
                 1797 
               
               
                 oak) 
                 1.0301 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Quercus alba  (White 
                 
                   Que a 
                 
                 No 
                 Pathogenesis-related 
                 ABZ81047 
                 B6RQS3 
                 1798 
               
               
                 oak) 
                 1.0401 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                 
                   Ricinus communis 
                 
                   Ric c  1 
                 Yes 
                 2S albumin 
                 CAA38097 
                 P01089 
                 1799 
               
               
                 (Castor bean) 
                   
                   
                   
                   
                   
                   
               
               
                   Rubus idaeus  (Red 
                   Rub i  1 
                 Yes 
                 Pathogenesis-related 
                 ABG54495 
                 Q0Z8U9 
                 1800 
               
               
                 raspberry) 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Rubus idaeus  (Red 
                   Rub i  3 
                 Yes 
                 Non-specific lipid transfer 
                 ABG54494 
                 Q0Z8V0 
                 1801 
               
               
                 raspberry) 
                   
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                 
                   Sal k 
                 
                 No 
                 Pectin methylesterase 
                 P83181 
                 P83181 
                 1802 
               
               
                 thistle, Saltwort) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                 
                   Sal k 
                 
                 No 
                 Pectinesterase 
                 AAT99258 
                 I6LD58 
                 1803 
               
               
                 thistle, Saltwort) 
                 1.0201 
                   
                   
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                 
                   Sal k 
                 
                 No 
                 Pectinesterase 
                 AAX11262 
                 Q17ST3 
                 1804 
               
               
                 thistle, Saltwort) 
                 1.0301 
                   
                   
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                 
                   Sal k 
                 
                 No 
                 Pectinesterase 
                 AAX11261 
                 Q17ST4 
                 1805 
               
               
                 thistle, Saltwort) 
                 1.0302 
                   
                   
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                   Sal k  2 
                 No 
                 Protein kinase homologue 
                 AAN05083 
                 Q8L5K9 
                 1806 
               
               
                 thistle, Saltwort) 
                   
                   
                   
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                   Sal k  3 
                 No 
                 Cobalamin independent 
                 ACO34814 
                 C1KEU0 
                 1807 
               
               
                 thistle, Saltwort) 
                   
                   
                 methionine synthase 
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                 
                   Sal k 
                 
                 No 
                 Profilin 
                 ACS34771 
                 C6JWH0 
                 1808 
               
               
                 thistle, Saltwort) 
                 4.0101 
                   
                   
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                 
                   Sal k 
                 
                 No 
                 Profilin 
                 ADK22841 
                 E2D0Y9 
                 1809 
               
               
                 thistle, Saltwort) 
                 4.0201 
                   
                   
                   
                   
                   
               
               
                   Salsola kali  (Russian 
                   Sal k  5 
                 No 
                 Ole e 1-like protein 
                 ADK22842 
                 E2D0Z0 
                 1810 
               
               
                 thistle, Saltwort) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sesamum indicum 
                 
                   Ses i  1 
                 Yes 
                 2S albumin 
                 AAK15088 
                 Q9AUD1 
                 1811 
               
               
                 (Sesame) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sesamum indicum 
                 
                   Ses i  2 
                 Yes 
                 2S seed storage protein 1 
                 AAD42943 
                 Q9XHP1 
                 1812 
               
               
                 (Sesame) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sesamum indicum 
                 
                   Ses i  3 
                 Yes 
                 7S vicilin-like globulin 
                 AAK15089 
                 Q9AUD0 
                 1813 
               
               
                 (Sesame) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sesamum indicum 
                 
                   Ses i  4 
                 Yes 
                 oleosin 
                 AAG23840 
                 Q9FUJ9 
                 1814 
               
               
                 (Sesame) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sesamum indicum 
                 
                   Ses i  5 
                 Yes 
                 oleosin 
                 AAD42942 
                 Q9XHP2 
                 1815 
               
               
                 (Sesame) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Sesamum indicum 
                 
                   Ses i  6 
                 Yes 
                 11S globulin seed storage 
                 AAD42944 
                 Q9XHP0 
                 1816 
               
               
                 (Sesame) 
                   
                   
                 protein 2 
                   
                   
                   
               
               
                 
                   Sesamum indicum 
                 
                   Ses i  7 
                 Yes 
                 11S globulin 
                 AAK15087 
                 Q9AUD2 
                 1817 
               
               
                 (Sesame) 
                   
                   
                   
                   
                   
                   
               
               
                   Sinapis alba  (Yellow 
                   Sin a  1 
                 Yes 
                 2S albumin 
                 CAA62909 
                 P15322 
                 1818 
               
               
                 mustard) 
                   
                   
                   
                   
                   
                   
               
               
                   Sinapis alba  (Yellow 
                   Sin a  2 
                 Yes 
                 11S globulin (legumin- 
                 AAX77383 
                 Q2TLW0 
                 1819 
               
               
                 mustard) 
                   
                   
                 like) seed storage protein 
                   
                   
                   
               
               
                   Sinapis alba  (Yellow 
                   Sin a  3 
                 Yes 
                 non-specific lipid transfer 
                 ABU95411 
                 E6Y2L9 
                 1820 
               
               
                 mustard) 
                   
                   
                 protein type 1 
                   
                   
                   
               
               
                   Sinapis alba  (Yellow 
                   Sin a  4 
                 Yes 
                 Profilin 
                 ABU95412 
                 E6Y2M0 
                 1821 
               
               
                 mustard) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                   Sola l  1 
                 Yes 
                 Profilin-2 
                 CAD10377 
                 Q93YG7 
                 1822 
               
               
                 ( Lycopersicon   
                   
                   
                   
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                 
                   Sola l 
                 
                 Yes 
                 Beta-fructofuranosidase 
                 AAL75449 
                 Q547Q0 
                 1823 
               
               
                 ( Lycopersicon   
                 2.0101 
                   
                   
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                 
                   Sola l 
                 
                 Yes 
                 Beta-fructofuranosidase 
                 AAL75450 
                 Q8RVW4 
                 1824 
               
               
                 ( Lycopersicon   
                 2.0201 
                   
                   
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                   Sola l  3 
                 Yes 
                 Non-specific lipid-transfer 
                 AAB42069 
                 P93224 
                 1825 
               
               
                 ( Lycopersicon   
                   
                   
                 protein 2 
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                 
                   Sola l 
                 
                 Yes 
                 Pathogenesis-related 
                 AHC08073 
                 K4CWC5 
                 1826 
               
               
                 ( Lycopersicon   
                 4.0101 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                 family member, TSI-1 
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                 
                   Sola l 
                 
                 Yes 
                 Pathogenesis-related 
                 NP_001275580 
                 K4CWC4 
                 1827 
               
               
                 ( Lycopersicon   
                 4.0201 
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                 family member, TSI-1 
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                   Sola l  5 
                 Yes 
                 Cyclophilin (Peptidyl- 
                 AAA63543 
                 P21568 
                 1828 
               
               
                 ( Lycopersicon   
                   
                   
                 prolyl cis-trans isomerase) 
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                   Sola l  6 
                 Yes 
                 Non-specific lipid transfer 
                 XP_004232333 
                 K4BBD9 
                 1829 
               
               
                 ( Lycopersicon   
                   
                   
                 protein type 2 (nsLTP2) 
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum lycopersicum 
                 
                   Sola l  7 
                 Yes 
                 nsLTP type 1 
                 / 
                 / 
                 / 
               
               
                 ( Lycopersicon   
                   
                   
                   
                   
                   
                   
               
               
                   esculentum ) (Tomato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum tuberosum 
                 
                   Sola t  1 
                 Yes 
                 Patatin 
                 CAA31576 
                 P15476 
                 1830 
               
               
                 (Potato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum tuberosum 
                 
                   Sola t  2 
                 Yes 
                 Cathepsin D inhibitor PDI 
                 P16348 
                 P16348 
                 1831 
               
               
                 (Potato) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Solanum tuberosum 
                 
                 
                   Sola t 
                 
                 Yes 
                 Cysteine protease inhibitor 
                 AAB63099 
                 O24383 
                 1832 
               
               
                 (Potato) 
                 3.0101 
                   
                 10 
                   
                   
                   
               
               
                 
                   Solanum tuberosum 
                 
                 
                   Sola t 
                 
                 Yes 
                 Cysteine protease inhibitor 
                 AAA33845 
                 P20347 
                 1833 
               
               
                 (Potato) 
                 3.0102 
                   
                 1 
                   
                   
                   
               
               
                 
                   Solanum tuberosum 
                 
                   Sola t  4 
                 Yes 
                 Serine protease inhibitor 7 
                 BAA04149 
                 P30941 
                 1834 
               
               
                 (Potato) 
                   
                   
                   
                   
                   
                   
               
               
                   Syringa vulgaris  (Lilac) 
                 
                   Syr v 
                 
                 No 
                 Ole e 1-related protein, 
                 S43243 
                 / 
                 1835 
               
               
                   
                 1.0102 
                   
                 isoform 2 
                   
                   
                   
               
               
                   Syringa vulgaris  (Lilac) 
                 
                   Syr v 
                 
                 No 
                 Olee 1-related protein, 
                 S43244 
                 / 
                 1836 
               
               
                   
                 1.0103 
                   
                 isoform 3 
                   
                   
                   
               
               
                   Syringa vulgaris  (Lilac) 
                   Syr v  3 
                 No 
                 Polcalcin 
                 AAK01144 
                 P58171 
                 1837 
               
               
                 
                   Triplochiton 
                 
                   Trip s  1 
                 No 
                 Endochitinase 
                 / 
                 C0HJM6 
                 1838 
               
               
                   scleroxylon  (Obeche) 
                   
                   
                   
                   
                   
                   
               
               
                   Vigna radiata  (Mung 
                   Vig r  1 
                 Yes 
                 Pathogenesis-related 
                 AAX19889 
                 Q2VU97 
                 1839 
               
               
                 bean) 
                   
                   
                 protein, PR-10,  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Vigna radiata  (Mung 
                 
                   Vig r 
                 
                 Yes 
                 8S Globulin (Vicilin), beta 
                 ABG02262 
                 Q198W3 
                 1840 
               
               
                 bean) 
                 2.0101 
                   
                 isoform 
                   
                   
                   
               
               
                   Vigna radiata  (Mung 
                 
                   Vig r 
                 
                 Yes 
                 8S globulin alpha subunit 
                 ABW23574 
                 B1NPN8 
                 1841 
               
               
                 bean) 
                 2.0201 
                   
                   
                   
                   
                   
               
               
                   Vigna radiata  (Mung 
                   Vig r  4 
                 Yes 
                 Seed albumin 
                 CAA50008 
                 Q43680 
                 1842 
               
               
                 bean) 
                   
                   
                   
                   
                   
                   
               
               
                   Vigna radiata  (Mung 
                   Vig r  6 
                 Yes 
                 Cytokinin-specific binding 
                 BAA74451 
                 Q9ZWP8 
                 1843 
               
               
                 bean) 
                   
                   
                 protein (CSBP),  Bet v  1 
                   
                   
                   
               
               
                   
                   
                   
                 family member 
                   
                   
                   
               
               
                   Vitis vinifera  (Grape) 
                   Vit v  1 
                 Yes 
                 Non-specific lipid transfer 
                 AAO33394 
                 Q850K5 
                 1844 
               
               
                   
                   
                   
                 protein 1 (nsLTP1) 
                   
                   
                   
               
               
                 
                   Ziziphus mauritiana 
                 
                   Ziz m  1 
                 Yes 
                 Class III chitinase 
                 AAX40948 
                 Q2VST0 
                 1845 
               
               
                 (Chinese-date) 
                   
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Plantae Pinopsida 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 
                   Chamaecyparis obtusa 
                 
                   Cha o  1 
                 No 
                 Pectate lyase 
                 BAA08246 
                 Q96385 
                 1846 
               
               
                 (Japanese cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Chamaecyparis obtusa 
                 
                   Cha o  2 
                 No 
                 Polygalacturonase 
                 Q7M1E7 
                 Q7M1E7 
                 1847 
               
               
                 (Japanese cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Chamaecyparis obtusa 
                 
                   Cha o  3 
                 No 
                 Cellulase (glycosyl 
                 / 
                 / 
                 / 
               
               
                 (Japanese cypress) 
                   
                   
                 hydrolase) 
                   
                   
                   
               
               
                 
                   Cryptomeria japonica 
                 
                 
                   Cry j 
                 
                 No 
                 Pectate lyase 
                 BAA05542 
                 P18632 
                 1848 
               
               
                 (Sugi) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Cryptomeria japonica 
                 
                 
                   Cry j 
                 
                 No 
                 Pectate lyase 
                 BAB86286 
                 Q8RUR1 
                 1849 
               
               
                 (Sugi) 
                 1.0103 
                   
                   
                   
                   
                   
               
               
                 
                   Cryptomeria japonica 
                 
                   Cry j  2 
                 No 
                 Polygalacturonase 
                 / 
                 / 
                 / 
               
               
                 (Sugi) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus arizonica 
                 
                   Cup a  1 
                 No 
                 Pectate lyase 
                 BAA06172 
                 P43212 
                 1850 
               
               
                 (Cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus 
                 
                 
                   Cup s 
                 
                 No 
                 Pectate lyase 
                 AAF72625 
                 Q9M4S6 
                 1851 
               
               
                   sempervirens  (Common 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus 
                 
                 
                   Cup s 
                 
                 No 
                 Pectate lyase 
                 AAF72626 
                 Q9M4S5 
                 1852 
               
               
                   sempervirens  (Common 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus 
                 
                 
                   Cup s 
                 
                 No 
                 Pectate lyase 
                 AAF72627 
                 Q9M4S4 
                 1853 
               
               
                   sempervirens  (Common 
                 1.0103 
                   
                   
                   
                   
                   
               
               
                 cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus 
                 
                 
                   Cup s 
                 
                 No 
                 Pectate lyase 
                 AAF72628 
                 Q9M4S3 
                 1854 
               
               
                   sempervirens  (Common 
                 1.0104 
                   
                   
                   
                   
                   
               
               
                 cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus 
                 
                 
                   Cup s 
                 
                 No 
                 Pectate lyase 
                 AAF72629 
                 Q9M4S2 
                 1855 
               
               
                   sempervirens  (Common 
                 1.0105 
                   
                   
                   
                   
                   
               
               
                 cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus 
                 
                   Cup s  2 
                 No 
                 Polygalacturonase 
                 / 
                 C0HKB1 
                 1856 
               
               
                   sempervirens  (Common 
                   
                   
                   
                   
                   
                   
               
               
                 cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus 
                 
                 
                   Cup s 
                 
                 No 
                 Thaumatin-like protein 
                 AAR21073 
                 Q69CS2 
                 1857 
               
               
                   sempervirens  (Common 
                 3.0101 
                   
                   
                   
                   
                   
               
               
                 cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Cupressus 
                 
                 
                   Cup s 
                 
                 No 
                 Thaumatin-like protein 
                 AAR21074 
                 Q69CS3 
                 1858 
               
               
                   sempervirens  (Common 
                 3.0102 
                   
                   
                   
                   
                   
               
               
                 cypress) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Juniperus ashei 
                 
                 
                   Jun a 
                 
                 No 
                 Pectate lyase 
                 AAD03608 
                 P81294 
                 1859 
               
               
                 (Mountain cedar) 
                 1.010101 
                   
                   
                   
                   
                   
               
               
                 
                   Juniperus ashei 
                 
                 
                   Jun a 
                 
                 No 
                 Pectate lyase 
                 AAD03609 
                 P81294 
                 1859 
               
               
                 (Mountain cedar) 
                 1.010102 
                   
                   
                   
                   
                   
               
               
                 
                   Juniperus ashei 
                 
                   Jun a  2 
                 No 
                 Polygalacturonase 
                 CAC05582 
                 Q9FY19 
                 1860 
               
               
                 (Mountain cedar) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Juniperus ashei 
                 
                   Jun a  3 
                 No 
                 Thaumatin-like protein 
                 AAF31759 
                 P81295 
                 1861 
               
               
                 (Mountain cedar) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Juniperus oxycedrus 
                 
                   Jun o  4 
                 No 
                 Polcalcin-like protein (4 
                 AAC15474 
                 O64943 
                 1862 
               
               
                 (Prickly juniper) 
                   
                   
                 EF hand domains) 
                   
                   
                   
               
               
                 
                   Juniperus sabinoides 
                 
                   Jun s  1 
                 No 
                 / 
                 / 
                 / 
                 / 
               
               
                 (Mountain cedar) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Juniperus virginiana 
                 
                 
                   Jun v 
                 
                 No 
                 Pectate lyase-1 
                 AAF80166 
                 Q9LLT2 
                 1863 
               
               
                 (Eastern red cedar) 
                 1.0101 
                   
                   
                   
                   
                   
               
               
                 
                   Juniperus virginiana 
                 
                 
                   Jun v 
                 
                 No 
                 Pectate lyase-1 
                 AAF80164 
                 Q9LLT1 
                 1863 
               
               
                 (Eastern red cedar) 
                 1.0102 
                   
                   
                   
                   
                   
               
               
                 
                   Juniperus virginiana 
                 
                   Jun v  3 
                 No 
                 Thaumatin-like protein 
                 AAF80167 
                 Q9LD79 
                 1864 
               
               
                 (Eastern red cedar) 
                   
                   
                   
                   
                   
                   
               
               
                 
                   Pinus koraiensis 
                 
                   Pin k  2 
                 No 
                 Vicilin 
                 AHC94918 
                 V9VGU0 
                 1865 
               
               
                 (Korean Pine) 
                   
                   
                   
                   
                   
                   
               
               
                   Pinus pinea  (Stone 
                   Pin p  1 
                 No 
                 2S albumin 
                 CTQ87571.1 
                 / 
                 1866 
               
               
                 pine) 
               
               
                   
               
            
           
         
       
     
     In some embodiments, SPNs, magnetic particle conjugates and compositions of the present invention may detect a specific epitope of a target allergen. As used herein, an “epitope” is the part of the allergen that is recognized by the immune system (e.g., antibodies. B cells, or T cells). Generally, the whole allergen is not involved in the immune response, but rather, the epitopes of allergens, which are recognized by a T-cell receptor or an IgE-antibody, contribute to allergic reactions. SPNs, magnetic particle conjugates and compositions of the present invention may bind to an epitope of a target allergen in a similar manner as antibodies or T-cell receptors. 
     Epitopes of an allergen protein may be identified using methods and techniques known in the art. For example, IgE-binding epitopes may be determined by methods such as X-ray co-crystallography of allergens and immunocomplexes, array-based oligo-peptide scanning, mutagenesis mapping, or nuclear magnetic resonance. For example, T-cell epitopes may be identified using short peptide fragments that overlap the entire amino acid sequence of the target allergen and allergen-specific T-cell lines derived from peripheral blood mononuclear cells. Peptides that induce T-cell proliferation contain T-cell epitopes. Additional techniques that may be used to determine epitopes on an allergen protein include crosslinking coupled mass spectrometry, hydrogen-deuterium exchange, and computer-based in silico analysis. Many epitopes of common food allergens have been identified, such as those listed in Tables 1-5 of Matsuo H et al.,  Allergol Int.  2015 October; 64(4):332-43. 
     In some embodiments, SPNs, magnetic particle conjugates and compositions of the present invention may be used in a hospital for clinical food allergy or allergy test and to identify food/allergen(s) to which a patient is allergic. In addition, SPNs, magnetic particle conjugates and compositions of the present invention may be used as a carry-on tester for people who have food/environmental allergy, for example at home to test commercial food, or at restaurant to check dishes they ordered. The food sample could be fresh food, frozen food, cooled food or processed food containing animal derived meat and/or vegetables. 
     In some embodiments, SPNs, magnetic particle conjugates and compositions of the present invention may detect other target molecules, including but not limited to, pathogens from a pathogenic microorganism in a sample, such as bacteria, yeasts, fungi, spores, viruses or prions; disease proteins (e.g., biomarkers for diseases diagnosis and prognosis); pesticides and fertilizers remained in the environment; and toxins. In other embodiments, SPNs and compositions of the present invention may bind to non-protein targets such as minerals and small molecules (e.g., antibiotics), drugs and inorganic ion. 
     II. Kits, Packaging and Biosensors 
     Compositions, SPNs, SPN-complement complexes, magnetic particle conjugates, DNA-printed solid substrates and detection agents of the present invention may be combined with other ingredients or reagents or prepared as components of kits or other retail products for commercial sale or distribution. 
     The kit will contain compositions of the present invention, along with instructions regarding administration and/or use of the kit. The kit may also contain one or more of the following: a syringe, a bag or bottle. 
     Formulations and/or compositions of the present invention can be packaged for use in a variety of pharmaceutically or diagnostically acceptable containers using any acceptable container closure, as the formulations are compatible with PVC-containing and PVC-free containers and container closures. Examples of acceptable containers include, but are not limited to, ampules and pre-filled syringes, cartridges and the like. 
     Alternatively, the formulation may contain SPNs, SPN-complement complexes, magnetic particle conjugates in one compartment of an admix bag and an acceptable solvent in a separate compartment of the admix bag such that the two compartments may be mixed together prior to use. Acceptable containers are well known in the art and commercially available. 
     In some embodiments, biosensors comprising compositions, SPN-complement complexes, magnetic particle conjugates, DNA-printed solid substrates and detection agents of the present invention are provided. The biosensor may be an on-chip magnetic particle biosensor. For example, a plurality of magnetic particle sensors are embedded in the integrated circuit; the magnetic particle sensors are capable of detecting magnetic particles specifically bound to the one or more sensor areas on the surface of the integrated circuit. The affinity molecules on the surface of the sensor area may be SPNs, or SPN-complement complexes of the present invention. 
     In some embodiments, a graphene-oxide (GO) based biosensor is provided, wherein the sensing agents are SPNs conjugated to the graphene-oxide surface. 
     In some aspects, it may be a “signal on” biosensor. The SPN and its complementary sequence may be labeled with a fluorophore and a quencher at one end of the nucleic acid sequence, respectively. The signal produced by the sensor is dependent on the structural changes in the SPNs following the binding of target allergens. The binding of the target allergen to the SPN disrupts the SPN-complement complex and replaces the complementary sequence in the complex, resulting in the quenched fluorescent signal back on ( FIG. 4B ). 
     In some aspects, it may be a “signal off” biosensor, in which the complementary sequence is labeled with a fluorophore at one end of the sequence. The binding of the target allergen to the SPN detaches the complementary sequence in the complex, causing the fluorescent signal off ( FIG. 4A ). 
     In other aspects, it may be a “dual signal” biosensor, in which the SPN and the complementary sequence are labeled with different fluorophores. The competition between the target allergen and the complement will change the fluorescent signals ( FIG. 4C ). 
     III. Detection Methods and Assays 
     In accordance with the present invention, aptamers, SPNs and SPN-complement complexes, magnetic particle conjugates, DNA-printed solid substrates, detection agents and compositions of the present invention may be used to, in a broad concept, detect any molecules in a sample in a large variety of applications, such as food safety, diagnostic and prognostic tests in civilian and battlefield settings, environmental monitoring/control, and military use for detection of biological weapons. Various methods and assays may be used in combination with aptamers, SPNs, SPN-complement complexes, magnetic particle conjugates, DNA-printed solid substrates, detection agents and compositions of the present invention, the choice may depend on the application field. 
     Particularly the present invention provides methods of determining the absence, presence and/or quantity of one or more target allergens in a sample using detection agents comprising SPN-magnetic particle conjugates and printed solid substrates. In some embodiments, the detection assays and methods can be used in a hospital for clinical food allergy or allergy test and to identify food/allergen(s) to which a patient is allergic. Such assays and methods may be used to monitor allergen contamination in food industry. Additionally, they may also be used at home or in a restaurant by a person who has allergy to test the allergen content before he/she consumes the food. 
     Examples of foods are eggs, milk, meat, fishes, crustacea and mollusks, cereals, legumes and nuts, fruits, vegetables, beer yeast, and gelatin; more particularly, egg white and egg yolk of the eggs, milk and cheese of the milk, pork, beef, chicken and mutton of the meat, mackerel, horse mackerel, sardine, tuna, salmon, codfish, flatfish and salmon caviar of the fishes, crab, shrimp, blue mussel, squid, octopus, lobster and abalone of the crustacea and mollusks, wheat, rice, buckwheat, rye, barley, oat, corn, millet, foxtail millet and barnyardgrass of the cereals, soybean, peanut, cacao, pea, kidney bean, hazelnut, Brazil nut, almond, coconut and walnut of the legumes and nuts, apple, banana, orange, peach, kiwi, strawberry, melon, avocado, grapefruit, mango, pear, sesame and mustard of the fruits, tomato, carrot, potato, spinach, onion, garlic, bamboo shoot, pumpkin, sweet potato, celery, parsley, yam and Matsutake mushroom of the vegetables, the foods containing them, and the ingredients thereof (e.g., ovoalbumin, ovomucoid, lysozyme, casein, beta-lactoglobulin, alpha-lactoalbumin, gluten, and alpha-amylase inhibitor). 
     The foods could be fresh foods, frozen foods, cooled foods or processed foods containing animal derived meat and/or vegetables. These foods may be processed by heating, freezing, drying, salting, fermentation, enzymatic processing, etc. 
     In some embodiments, more than one agents may be used, depending on the nature of the food matrixes. Some food contains several allergenic proteins, e.g., at least eight peanut proteins, such as Ara h1 and Ara h2, can potentially cause an immunological response. In such case, more than one SPNs against more than one allergenic protein may be used in a mixed cocktail for detecting the absence or presence of peanut. In other aspects, some food matrixes such as fish, shellfish and mollusks, contain only one major allergenic protein. One or more SPNs that specifically bind to this major allergen protein may be used for allergen detection. 
     In some embodiments, allergen detection assays and methods of the present invention can detect a lower concentration of allergen in a food sample. The sensitivity of nucleic acid aptamers makes it possible to detect the presence of an allergen as low as 0.0001 ppm. In some aspects, the concentration or mass of allergen that can be detected may range from 0.001 ppm to 5 ppm, or from 0.001 ppm to 0.1 ppm, or from 0.1 ppm to 3 ppm, or from 1 ppm to 5 ppm, or from 5 ppm to 10 ppm, or from 10 ppm to 50 ppm, or from 10 ppm to 20 ppm, or from 10 ppm to 100 ppm. In some aspects, the concentration or mass of allergen in a food sample that can be detected may be 0.001 ppm, 0.002 ppm, 0.003 ppm, 0.004 ppm, 0.005 ppm, 0.006 ppm, 0.007 ppm, 0.008 ppm, 0.009 ppm, 0.01 ppm, 0.02 ppm, 0.03 ppm, 0.04 ppm, 0.05 ppm, 0.06 ppm, 0.07 ppm, 0.08 ppm, 0.09 ppm, 0.1 ppm, 0.2 ppm, 0.3 ppm, 0.4 ppm, 0.5 ppm, 0.6 ppm, 0.7 ppm, 0.8 ppm, 0.9 ppm, 1.0 ppm, 1.5 ppm, 2 ppm, 2.5 ppm, 3 ppm, 3.5 ppm, 4 ppm, 4.5 ppm, 5 ppm or 10 ppm. In some embodiments, the concentration or mass of allergen in a food sample that can be detected may be 5 ppm, 1 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm, 50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm, 95 ppm or 100 ppm. In one embodiment, the concentration or mass of allergen in a food sample that can be detected may be at low as 50 ppm. In another embodiment, the concentration or mass of allergen in a food sample that can be detected may be at low as 12.5 ppm 
     In accordance with the present aptamer derived SPN based detection assays, the sensitivity can be achieved regardless of food matrices. The diverse food matrices with different salt, fat and sugar content, different color, texture, and pH can be detected at the present sensitivity. 
     In some embodiments, methods of the present invention for detecting the absence, presence and/or quantity of a target allergen in a test sample comprise (a). obtaining and processing a test sample which is suspected to contain an allergen of interest; (b). contacting the processed test sample with detection agents comprising SPNs conjugated to solid substrates, wherein the SPNs are single-stranded nucleic acid molecules having 20 to 200 nucleotides capable of specifically binding to the target allergen; (c) washing the mixture of the detection agents and the test sample formed in step (b); (d). detecting the allergen-SPN complexes formed during the assay; and (e). processing and analyzing the detection signals to determine whether the target allergen is present in the food sample and/or the quantity of the target allergen in the sample. 
     In some aspects, the detection agents further comprise a nucleic acid molecule complementary to a region of the SPN sequence which binds to the SPN only when the SPN is not bound to its target allergen protein; the SPN and the complementary sequence forms the SPN-complement complex, in which the SPN and the complementary sequence work as binding ligand (to the target allergen) and detection molecule or label, respectively. In some examples, the SPN is covalently immobilized on the surface of the solid substrate through its 5′end or 3′end. In other examples, the complementary sequence is covalently immobilized on the surface of the solid substrate. 
     In some examples, the solid substrate is magnetic particles. In accordance, the magnetic particles may be held using a magnetic field during the wash step. Optionally, the washed reaction mixture may be shaken, prior to detecting the allergen-SPN-magnetic particle complexes formed during the assay. 
     Assays and methods of the present invention may have various forms depending on biosensors, detection kits and detection devices and systems used to implement the assays. SPN/complement-magnetic particle conjugates may be used in any steps of the assays, such as capture agents to capture a target analyte (e.g., a target allergen or a specific epitope of the target allergen) in a sample; or detector agents for signaling detection; or competitive binding agents, or affinity agents to selected a target analyte (e.g., an allergen) bound magnetic particles. 
     In some embodiments, SPNs comprise the nucleic acid sequences of SEQ ID NOs.: 1-696 (See Tables 1-4). The complementary sequences may contain about 5-20 nucleotide residues. The SPN and its complementary sequence may be at a ratio of 1:5, or at a ratio of 1:4, or at a ratio of 1:3, or at a ratio of 1:2. 
     In some embodiments, an attachment assay may be developed using the compositions and agents of the present invention (as shown in  FIG. 6A ). Accordingly, the assay may comprise steps of (a). obtaining and processing a test sample which is suspected to contain an allergen of interest; (b). contacting the processed sample with signaling polynucleotides (SPNs) which specifically bind to the allergen of interest, wherein the SPNs are labeled with a fluorophore at one end of the sequence; (c). exposing the mixture of the processed sample and the SPNs formed in step (b) to the surface of a solid substrate, wherein the surface of the solid substrate comprises nucleic acid sequences that are complementary to a region of the SPN sequence; (d). washing the mixture formed in step (c); (e). detecting a fluorescent signal from the surface of the solid substrate; and (f). processing and analyzing the fluorescent signal to determine the absence, presence, and/or the quantity of the allergen of interest in the test sample. The free SPNs which are not bound to the allergen proteins will bind to the complementary sequences printed on the solid surface and will contribute to the fluorescence reading, while the SPNs which are bound to the allergen proteins will not attach to the solid surface and will be washed away. The fluorescence reading will indicate the absence, presence or quantity of the allergen of interest in the test sample. 
     In other embodiments, a detachment assay may be developed using the compositions and agents of the present invention (as shown in  FIG. 6B ). Accordingly, the assay may comprise steps of (a) obtaining and processing a test sample which is suspected to contain an allergen of interest; (b) exposing the processed sample to the surface of a solid substrate, wherein the surface of the solid substrate comprises SPN-complement complexes which specifically bind the allergen of interest; (c) washing the mixture formed in step (b); (d) detecting a fluorescent signal from the surface of the solid substrate and comparing the fluorescent signal to that detected prior to the exposure of the processed sample; and (e) processing and analyzing the fluorescent signal to determine the absence, presence, and/or the quantity of the allergen of interest in the test sample. In accordance with this detachment assay, the SPN complementary sequences are printed to the surface of the solid substrate. The SPNs which specifically bind to the allergen of interest are incubated with the surface. The mixture will be washed and a fluorescence reading is recorded. The allergen proteins in the test sample, when added to the SPN-complement complexes, will bind to the SPNs and the SPN-protein complexes detach from the complementary sequences and will be washed away. The fluorescence reading from step (d) is compared with the preread fluorescence signal; the difference may be used for determining the absence, presence or quantity of the allergen of interest in the test sample. 
     In some embodiments, SPNs with different sequences but having a high affinity and specificity to the same target allergen may be used in combination. In other aspects, SPNs of the present invention may be used in combination with antibodies that bind the same target allergen in a detection assay. 
     In some embodiments, the detection assay of the present invention can be optimized depending on the testing condition such as food matrix. The reaction buffer used may be decreased in volume. In some aspects. The reaction volume can be limited to less than 5 mL, for example, less than 4 mL, or less than 3 mL, or less than 2 mL, or less than 1 mL. In one example, the volume may be 1.5 mL, 2 mL, 2.5 mL, 3 mL, 3.5 mL, 4.5 mL, and 5.0 mL. 
     In some embodiments, the sample size can be reduced, limiting the requirement of a large size sample. The assay sensitivity can be achieved with a small portion of test sample, for example, less than 5 mg, or less than 4.5 mg, or less than 4 mg, or less than 3.5 mg, or less than 3 mg, or less than 2.5 mg, or less than 2 mg, or less than 1.5 mg. 
     In some embodiments, the detection assay of the present invention can be completed in a short period of time, for example less than 5 minutes. In some aspects, the detection reaction can be completed from 10 seconds to 5 minutes, or from 10 seconds to 60 seconds, or from 1 minute to 2 minutes. In some examples, the detection assay can be completed in about 10 seconds, about 20 seconds, about 30 seconds, about 35 seconds, about 40 seconds, about 45 seconds, about 50 seconds, about 1 minute, about 1.5 minutes, about 2 minutes, about 2.5 minutes, about 3 minutes, about 4.5 minutes, or about 5 minutes. 
     In some embodiments, the detection assays of the present invention can be optimized to achieve the greatest signal intensity. The step optimization may include, but are not limited to, changing the concentration of SPN molecules, controlling the sample and SPN incubation conditions and time, controlling the flow rate and maximizing the exposure of solid surface to SPNs. 
     In some embodiments, the detection assay of the present invention further comprising an internal control signal. The built-in control signal may be measured using pre-incubated free SPNs and/or free food samples without SPN binding. These control signals will reduce the background signal and non-specific reading during the detection reaction. 
     In some embodiments, the detection signals are processed and displayed by a reader device. The reader device may be a computer, an iPad and/or a cellphone, or other processors that can execute one or more methods for detecting the absence, presence and/or quantity of the target allergen. 
     In accordance with the present invention, the detection agents and other reaction agents of the detection assay are stable and can be stored for a long-period of time. In general, short nucleic acid molecules are stable at routine storage condition. In some aspects, the SPNs, SPN-magnetic bead conjugates and nucleic acid coated solid surfaces are stable and can maintain their activity for at least one month, or at least two months, or at least three months, or at least four months, or at least five months, or at least six months. They may be stable from about one month to about one year. The reaction agents such as the extraction buffer can be stable at various temperature for at least nine months, or at least ten months, or at least one year. 
     IV. Definitions 
     At various places in the present specification, substituents of compounds of the present disclosure are disclosed in groups or in ranges. It is specifically intended that the present disclosure include each and every individual sub-combination of the members of such groups and ranges. The following is a non-limiting list of term definitions. 
     Activity: As used herein, the term “activity” refers to the condition in which things are happening or being done. Compositions of the invention may have activity and this activity may involve the binding to a target molecule. 
     Allergen: as used herein, the term “allergen” means a compound, substance or composition that causes, elicits or triggers and immune reaction in a subject. As such, allergens are typically referred to as antigens. An allergen is typically a protein or a polypeptide. 
     Allergen detection agent: As used herein, the term “an allergen detection agent” refers to Any agent which is capable of, or does, interact with and/or bind to one or more allergens in a way that allows detection of such allergen in a sample is referred to herein as an “allergen detection agent” or “detection agent”. 
     Analyte: As used herein, an “analyte” is a target of interest that can specifically interact with (bind to) an aptamer and be detected and/or measured. In the context of the present invention, an analyte may be an allergen. 
     Aptamer: as used herein, the term“aptamer” refers to single stranded nucleic acid. In general, aptamers refer to either an oligonucleotide of a single defined sequence or a mixture of said oligonucleotides, wherein the mixture retains the properties of binding specifically to a target allergen. A RNA aptamer is an aptamer comprising ribonucleoside units. RNA aptamer also meant to encompass RNA analogs as defined herein. A DNA aptamer an aptamer comprising deoxy-ribonucleoside units. DNA aptamer also meant to encompass DNA analogs as defined herein. 
     Binding affinity: as used herein, the term “binding affinity” is intended to refer to the tendency of an aptamer to bind or not bind a target and describes the measure of the strength of the binding or affinity of the aptamer to bind the target. 
     Complementary: As used herein, the term “complementary” or “complement” refer to the natural binding of polynucleotides by base pairing such as A-T(U) and C-G pairs. Two single-stranded molecules may be partially complementary such that only some of the nucleic acids bind, or it may be “complete,” such that total complementarity exists between the single stranded molecules. The degree of complementarity between nucleic acid strands has significant effects on the efficiency and strength of the hybridization between the nucleic acid strands. 
     Detection: As used herein, the term “detection” means an extraction of a particular target protein from a mixture of many non-target proteins, indicating the absence, presence, and/or amount of a target protein from a mixture of many non-target proteins. 
     Hybridization: As used herein, the term “hybridization” refers to the process by which a polynucleotide strand anneals with a complementary strand through base pairing under defined hybridization conditions. Specific hybridization is an indication that two nucleic acid sequences share a high degree of identity such as a SPN of the present invention and a short complementary sequence of the SPN. Specific hybridization complexes form under permissive annealing conditions and remain hybridized after the “washing” step(s). The washing step(s) is particularly important in determining the stringency of the hybridization process, with more stringent conditions allowing less non-specific binding. i.e., binding between pairs of nucleic acid strands that are not perfectly matched. 
     Magnetic particles: As used herein, the term “magnetic particles” refer to ( ). Magnetic particles may include magnetic microbeads and/or nanoparticles. 
     Oligonucleotide: as used herein, the term “oligonucleotide” is generic to polydeoxyribonucleotides (containing 2′-deoxy-D-ribose or modified forms thereof), i.e. DNA, to polyribonucleotides (containing D ribose or modified forms thereof), i.e. RNA, and to any other type of polynucleotide which is an N-glycoside or C-glycoside of a purine or pyrimidine base, or modified purine or pyrimidine base or abasic nucleotides. In the context of the present invention, the “oligonucleotide” includes not only those with conventional bases, sugar residues and internucleotide linkages, but also those that contain modifications of any or all of these three moieties. 
     As used herein, the terms “nucleic acid” “polynucleotide” and “oligonucleotide” are used interchangeable herein and refer to a deoxyribonucleotide or ribonucleotide polymer in either single- or double-stranded form, and unless otherwise limited, encompasses known analogs of natural nucleotides that hybridize to nucleic acids in a manner similar to naturally-occurring nucleotides. Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl phosphonates, 2-O-methyl ribonucleotides, and peptide-nucleic acids (PNAs). 
     Sample: As used herein, the term “sample” refers to any composition that might contain a target of interest to be analyzed including, but not limited to, biological samples obtained from subjects (including humans and animals as detailed below), samples obtained from the environment for example soil samples, water samples, agriculture samples (including plant and crop samples), or food samples. Food samples may be obtained from fresh food, processed/cooked food or frozen food. 
     Sensitivity: As used herein, the term “sensitivity” means the ability of a detection molecule to bind to a target molecule. 
     Specifically binds: as used herein, the term “specifically binds” means that an aptamer reacts or associates more frequently, more rapidly, with greater duration and with greater affinity with a particular target molecule, than it does with alternative target molecules. For example, an aptamer that specifically binds to a target allergen binds that allergen or a structural part or fragment thereof with greater affinity, avidity, more readily, and/or with greater duration than it binds to unrelated allergen protein and/or parts or fragments thereof. It is also understood by reading this definition that, for example, an aptamer that specifically binds to a first target may or may not specifically bind to a second target. As such. “specific binding” does not necessarily require exclusive binding or non-detectable binding of another molecule, this is encompassed by the term “selective binding”. The specificity of binding is defined in terms of the comparative dissociation constants (Kd) of the aptamer for target as compared to the dissociation constant with respect to the aptamer and other materials in the environment or unrelated molecules in general. Typically, the Kd for the aptamer with respect to the target will be 2-fold, 5-fold, or 10-fold less than the Kd with respect to the target and the unrelated molecule or accompanying molecule in the environment. Even more preferably, the Kd will be 50-fold, 100-fold or 200-fold less. 
     Target: as used herein, the term “target” and “target molecule” refers to a molecule which may be found in a tested sample and which is capable of binding to a detection molecule such as an aptamer or an antibody. 
     EQUIVALENTS AND SCOPE 
     Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments in accordance with the invention described herein. The scope of the present invention is not intended to be limited to the above Description, but rather is as set forth in the appended claims. 
     In the claims, articles such as “a,” “an,” and “the” may mean one or more than one unless indicated to the contrary or otherwise evident from the context. Claims or descriptions that include “or” between one or more members of a group are considered satisfied if one, more than one, or all of the group members are present in, employed in, or otherwise relevant to a given product or process unless indicated to the contrary or otherwise evident from the context. The invention includes embodiments in which exactly one member of the group is present in, employed in, or otherwise relevant to a given product or process. The invention includes embodiments in which more than one, or the entire group members are present in, employed in, or otherwise relevant to a given product or process. 
     It is also noted that the term “comprising” is intended to be open and permits but does not require the inclusion of additional elements or steps. When the term “comprising” is used herein, the term “consisting of” is thus also encompassed and disclosed. 
     Where ranges are given, endpoints are included. Furthermore, it is to be understood that unless otherwise indicated or otherwise evident from the context and understanding of one of ordinary skill in the art, values that are expressed as ranges can assume any specific value or subrange within the stated ranges in different embodiments of the invention, to the tenth of the unit of the lower limit of the range, unless the context clearly dictates otherwise. 
     In addition, it is to be understood that any particular embodiment of the present invention that falls within the prior art may be explicitly excluded from any one or more of the claims. Since such embodiments are deemed to be known to one of ordinary skill in the art, they may be excluded even if the exclusion is not set forth explicitly herein. Any particular embodiment of the compositions of the invention (e.g., any antibiotic, therapeutic or active ingredient; any method of production, any method of use; etc.) can be excluded from any one or more claims, for any reason, whether or not related to the existence of prior art. 
     It is to be understood that the words which have been used are words of description rather than limitation, and that changes may be made within the purview of the appended claims without departing from the true scope and spirit of the invention in its broader aspects. 
     While the present invention has been described at some length and with some particularity with respect to the several described embodiments, it is not intended that it should be limited to any such particulars or embodiments or any particular embodiment, but it is to be construed with references to the appended claims so as to provide the broadest possible interpretation of such claims in view of the prior art and, therefore, to effectively encompass the intended scope of the invention. 
     EXAMPLES 
     Example 1: Conjugating Aptamers (Specific to Peanut Allergen Ara H1) to Magnetic Beads 
     An aptamer against the Ara h 1 allergen protein which is described by Tran et al. in  Selection of aptamers against Ara h  1  protein for FO - SPR biosensing of peanut allergens in food matrices . Biosensors and Bioelectronics, 2013, 43, 245-251 (incorporated herein by reference in its entirety), was used to design a signaling polynucleotide and conjugated to magnetic beads. The sequence of this aptamer is shown below (SEQ ID NO: 96). 
     5′TCGCACATTCCGCTTCTACCGGGGGTCGAGCTGAGTGGATGCGAA TCTGTGGGTGGGCCGTAAGTCCGTGTGTGCGAA3′ (SEQ ID NO.: 96) 
     A short nucleic acid sequence containing 5 nucleotide residues was designed to be complementary to the 3′-end residues of the T-modified aptamer of SEQ ID NO: 96. Alternatively, the sequence may be complementary to the 5′-end residues of the T-modified aptamer of SEQ ID NO: 96. One end of the complementary sequence was labeled with a fluorophore, Texas Red. 
     Three reactions were used to conjugate the aptamer to magnetic beads. The sequence was modified with a thiol (—SH) modifier at either the 5′end or 3′end ( FIG. 1A  and  FIG. 1B ). Magnetic beads were then linked to the 5′end (as shown in  FIG. 1A ) or to the 3′end (as shown in  FIG. 1B ) of the aptamer through covalent bonds. In particular, the thiol-modified aptamer, including 5′thiol and 3′thiol, was linked to magnetic beads by maleimide linkage. The Texas Red labeled complementary sequence was annealed to form a double stranded hybrid at the other end of the aptamer conjugated to magnetic beads, generating a magnetic bead conjugated signaling polynucleotide (SPN) (See  FIG. 1A  and  FIG. 1B ). 
     Alternatively, the aptamer was modified with a primary amine group at the 5′end or 3′end. The amine modified aptamer was attached to magnetic beads via carbodiimide chemical reaction. Magnetic carboxylated speedbeads were used (GE Healthcare Life Science, Cat. Log No.: XX). The Texas Red labeled complementary sequence was annealed to the aptamer at the 3′end, forming a magnetic bead conjugated signaling polynucleotide (SPN) (shown in  FIG. 1C ). Alternatively, the 5-nt complementary sequence was labeled with either Cy5 or Alex647 at one end (5′end or 3′end). 
     Amine Reaction 
     Amine-modified DNA molecules at either 5′ end of 3′ end were coupled to magnetic carboxylated speedbeads (GE Healthcare, USA) through carbodiimide chemical reaction. Carboxyl modified beads were first incubated with EDC/NHS (N-hydroxysuccinimide) prior to the addition of amine-modified DNA molecules. After EDC/NHS reaction, the carboxyl groups (—COOH) were activated for direct reaction with primary amines via amide bond formation. Amine-modified DNA molecules were mixed with the beads and the coupling reaction of beads and DNA occurred in water. After the reaction, a TRIS buffer was used to block/occupy the unreacted sites. 
     Thiol Reaction 
     Thiol-modified DNA molecules at either 5′ end of 3′ end were coupled to magnetic carboxylated speedbeads (GE Healthcare. USA) through amino-polyethylene-glycol maleimide linkage (2HN-PEG-Maleimide). The carboxyl groups (—COOH) on the surface of beads were activated first using water soluble EDC (1-ethyl-3-(˜3-dimethylaminopropyl) carbodiimide hydrochloride) and then by 2-HN-methoxypolyethylene glycol (PEG). The beads were incubated with EDC and PEGs in MES (4-morpholinoethanesulfonic acid) reaction buffer for 2 hours (30 min×4 sonicator bath). Activated beads were then mixed with thiol-modified DNA molecules and incubated with DTT (Dithiothreitol) in water. 
     Example 2: Reading of Fluorescence Signaling for Magnetic Beads Conjugated SPN in Detection of a Target Molecule 
     The study tested an experimental procedure for obtaining fluorescence readings and thereby determining the effectiveness of signaling polynucleotides in identifying and quantifying a molecular target such as an allergen protein in a food sample. 
     Peanut was diluted to an appropriate concentration in double-distilled water, ranging from 0 ppm to 500 ppm. SPN-magnetic bead conjugates as described in Example 1 were diluted in solution/buffer either HEPES/Tween, PBS/BSA or T-buffer (Tris pH8 100 mM NaCl, 50 mM KCl, 10 mM MgCl 0.5% Sodium Deoxcholate 0.1% Gelatin 1% Triton 0.1% SDS) at various concentrations. Peanut samples and SPN-magnetic bead conjugates were mixed and incubated for a period of time. The fluorescent signals before and after the reaction were detected and measured. In one study in which magnetic beads and SPN-complement were configured as illustrated in  FIG. 1A , the fluorescent signals from Texas Red dye was decreased after the allergen binding (shown in  FIGS. 2A to 2C ). The relative fluorescent unit (RFU) indicates that Texas Red signal is dropped off after the peanut allergen target binds the SPN in a concentration dependent manner. It also shows that the complementary sequence could affect the signal decrease. As seen in  FIGS. 2A and 2B , the complementary sequence could affect the signaling quencher after the binding of the target allergen. The sequence comprising 5 complementary nucleotides and 5nt polyA displayed a significantly fluorescence off (92% decrease) (C3 in  FIG. 2B ), while the sequence comprising 15 (C1 in  FIG. 2A ) or 10 (C2 in  FIG. 2A ) complementary nucleotides displays a moderate signal decrease. 
     Food samples spiked with peanut including Poptart, Graham crackers, Oreo, Blue Frosting were tested for signal decrease using 5′thiol modified SPN conjugated magnetic beads. As illustrated in  FIG. 3  and  FIG. 4 , 5′ thiol modification displays significant fluorescent signal decrease in all foods tested and at different concentrations of target allergen, peanut ( FIG. 3 ). 
     Comparison of signal detection using magnetic beads conjugated with 5′thiol modified SPNs and magnetic beads conjugated with polyA3′thiol modified SPNs suggest that 5′thiol modification displays more significant decrease than polyA3′thiol modification (See Table 6). About 40% of signal decrease was detected with magnetic beads conjugated with 5′thiol modified SPNs, while only about 20% of signal decrease was detected with magnetic beads conjugated with polyA3′thiol modified SPNs. The fluorescent measure also indicates that thiol modification displays more significant decrease than amine modification (data not shown). 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Fluorescent signal measure (RFU) (5′thiol v. 3′polyA thiol) 
               
            
           
           
               
               
               
            
               
                   
                 Peanut (ppm) 
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 0.0 
                 0.5 
                 5 
                 50 
                 500 
               
               
                   
                   
               
            
           
           
               
            
               
                 Magnetic beads with 5′thiol modified aptamers 
               
            
           
           
               
               
               
               
               
               
            
               
                 100 nM 
                 19394.5 
                 12896.9 
                 9233.6 
                 9376.4 
                 8885.3 
               
               
                  1 μM 
                 34037.8 
                 33582.8 
                 32449.6 
                 31283.4 
                 30118.7 
               
               
                  2.5 μM 
                 36535.8 
                 34252.9 
                 33767.1 
                 33926.5 
                 32535 
               
            
           
           
               
            
               
                 Magnetic beads with PolyA 3′thiol modified aptamers 
               
            
           
           
               
               
               
               
               
               
            
               
                 100 nM 
                 18888.4 
                 15053.6 
                 15249.9 
                 15554.5 
                 15387.1 
               
               
                  1 μM 
                 25213.2 
                 25691.6 
                 25303.1 
                 24456.2 
                 24404.7 
               
               
                  2.5 μM 
                 36515.3 
                 34852.5 
                 34342.5 
                 32904 
                 34064.1 
               
               
                   
               
            
           
         
       
     
     Example 3: Amine-SPN Conjugates 
     As described in Example 1, amine-modified SPNs were annealed with the 5-nt complementary sequences (at a ratio of 1:5). The SPN-complement complex was conjugated to magnetic beads. The complementary sequence was labeled with Cy5 or Alex647, respectively. The SPN-magnetic bead conjugates were incubated with different concentrations of peanut flour (from 0 ppm to 500 ppm) at 37° C. Fluorescent signals were recorded after the incubation. As shown in Table 7, both dyes display significant signal decreases as peanut concentration increases, indicating more allergen proteins bind the SPN and detach the complementary sequences from the SPNs. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Amine-SPN with peanut 
               
            
           
           
               
               
            
               
                   
                 Peanut 
               
            
           
           
               
               
               
               
               
               
            
               
                 SPN 
                 0 ppm 
                 0.5 ppm 
                 5 ppm 
                 50 ppm 
                 500 ppm 
               
               
                   
               
            
           
           
               
            
               
                 Alex647 
               
            
           
           
               
               
               
               
               
               
            
               
                 500 nM 
                 9026 
                 11617 
                 11211 
                 13074 
                 10112 
               
               
                  2.5 nM 
                 22583 
                 17662 
                 21953 
                 21380 
                 18007 
               
            
           
           
               
            
               
                 Cy5 
               
            
           
           
               
               
               
               
               
               
            
               
                 500 nM 
                 8102 
                 6600 
                 7762 
                 9364 
                 7431 
               
               
                  5 nM 
                 40595 
                 39622 
                 38021 
                 32525 
                 30101 
               
               
                   
               
            
           
         
       
     
     Example 4: Thiol-SPN Conjugates 
     As described in Example 1, thiol-modified oligonucleotides were annealed with the 5-nt complementary sequences (at a ratio of 1:5). The SPN-complement complexes were conjugated to magnetic beads. The complementary sequence was labeled with Cy5 or Alex647, respectively. The SPN-magnetic bead conjugates were incubated with different concentrations of peanut flour (from 0 ppm to 500 ppm) at 37° C. Fluorescent signals were recorded after the incubation. As shown in Table 8, both dyes display significant signal decreases as peanut concentration increases, indicating more allergen proteins bind the SPN and detach the complementary sequences from the SPNs. As shown in Table 8, when the SPN is at high concentration, 1000 nM, Cy5 labeled signal decreases as the peanut concentration increases. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Thiol-SPN with peanut 
               
            
           
           
               
               
            
               
                   
                 Peanut 
               
            
           
           
               
               
               
               
               
               
            
               
                 SPN 
                 0 ppm 
                 0.5 ppm 
                 5 ppm 
                 50 ppm 
                 500 ppm 
               
               
                   
               
            
           
           
               
            
               
                 Alex647 
               
            
           
           
               
               
               
               
               
               
            
               
                  500 nM 
                 14806.8 
                 15377.6 
                 15231.7 
                 14362.7 
                 15001.6 
               
               
                 1000 nM 
                 35980.7 
                 37720.1 
                 36920 
                 34901.3 
                 36660.1 
               
            
           
           
               
            
               
                 Cy5 
               
            
           
           
               
               
               
               
               
               
            
               
                  500 nM 
                 20692.3 
                 20838.9 
                 27197.2 
                 28255.5 
                 27519.7 
               
               
                 1000 nM 
                 28789.8 
                 28290.8 
                 20019.6 
                 20162.1 
                 19975 
               
               
                   
               
            
           
         
       
     
     Example 5: Examining the Effect of Washing Step and Reaction Conditions on Signal Detection 
     Various parameters may affect the stability of nucleic acids immobilized on magnetic beads and their interaction with target allergens in a test sample. Tables 9 and 10 illustrate the fluorescent reading in various conditions tested. The data suggests that doubling Maleimide concentration enables signal decrease using a higher concentration of SPN. However the decrease doesn&#39;t retain more than 50% (Table 9). Shaking the SPN solution prior to the signal reading to re-suspend magnetic beads is critical for accurate signal measurement. It can cause about 65% decrease in signal (Table 10). The data also suggests that the step of washing beads after reaction, extraction buffer and annealing conditions affect the signal detection (Table 10). 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Fluorescent signal measure (RFU) (2XMaleimide/40 mg) 
               
               
                 Magnetic beads with 5′thiol modified aptamers 
               
            
           
           
               
               
               
            
               
                   
                 Peanut (ppm) 
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 0.0 
                 5 
                 50 
                 500 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 100 
                 nM 
                 30786.4 
                 15740.5 
                 17604.1 
                 17452.1 
               
               
                 1 
                 μM 
                 27295.6 
                 20574 
                 15591.3 
                 15271.4 
               
               
                 5 
                 μM 
                 47497.8 
                 43324.9 
                 42758.1 
                 38351.5 
               
               
                   
               
            
           
         
       
     
                     TABLE 10                  Fluorescent signal measure (RFU)       Magnetic beads with 5′thiol modified aptamers                         Peanut (ppm)                                         0.0   0.5   5   50   500                                                 Extraction buffer; shaken   6152.8   4635   5170   5713   5304.5       before read       PBS with BSA; shaken   30504.5   10861.3   7620.5   6253.7   7037       before read       Exaction buffer; not   7051   5584.5   7660.8   6642.8   7567.3       shaken       PBS with BSA; not   8966   7685.8   8983   7269   9062.3       shaken                    
Effect of Wash Components on Signal Detection
 
     Reaction mixtures comprising polyA-thiol modified SPNs and peanuts were washed before signal detection using PBS buffer containing BSA (Bovine serum albumin). As compared with the signal after washing with PBS only, noticeable signal decrease is detected as peanut concentration is increased, indicating that BSA can facilitate removal of the complementary sequences after allergen binding (Table 11). 
                     TABLE 11               BSA effect on signal detection                  Peanut diluted in PBS/BSA                             SPN                                                 500 nM   2.5 nM   500 nM   2.5 nM           peanut   (Alex647)   (Alex647)   (Cy5)   (Cy5)                        0 ppm   16,692   18587   14458   33500            5 ppm   16832   18078   13304   34606           500 ppm   16731   17841   12874   37788                             Peanut diluted in PBS only (no BSA)                             SPN                                                 500 nM   2.5 nM   500 nM   2.5 nM               (Alex647)   (Alex647)   (Cy5)   (Cy5)                        0 ppm   19953.9   21356.3   13589.4   39577.4            5 ppm   16865.6   19508   14558.3   41638.4           500 ppm   16135.1   17034   12959.4   33267.1                        
Washing Buffer Containing Detergent
 
     SPN-complement complexes with Texas Red, complementary sequences labeled with Texas red, and fluorophore Texas Red alone were washed with PBS buffer containing Tween-20 at various concentrations. The fluorescent signals (RFU) after washing were detected and compared. 
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 signal (RFU) after PBS/Tween-20 washing 
               
            
           
           
               
               
               
               
            
               
                   
                 Amine- 
                 Complement 
                   
               
               
                   
                 SPN/complement 
                 alone 
                 Texas red 
               
               
                   
                   
               
            
           
           
               
               
               
               
            
               
                 PBS only 
                 13425.4 
                 5554.9 
                 1778.8 
               
               
                 PBS plus 0.05% Tween 
                 11249.8 
                 5133.5 
                 1581.3 
               
               
                 PBS plus 0.1% Tween 
                 5683 
                 2342.9 
                 903 
               
               
                   
               
            
           
         
       
     
     Example 6: Ratio of Oligonucleotides and Complementary Sequences 
     As shown in Examples 3 and 4, the high ratio 1:5 of aptamer oligonucleotides and complementary sequences gives a high fluorescent signal. A ratio 1:2 of aptamer oligonucleotides and complementary sequences was tested for signal detection. 
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 SPN: complementary sequence at a ratio of 1:2 
               
            
           
           
               
               
               
               
            
               
                 SPN 
                   
                 Alex647 
                 Cy5 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 100 
                 nM 
                 2167.5 
                 1809.9 
               
               
                 500 
                 nM 
                 14967 
                 10499.3 
               
               
                 1 
                 μM 
                 29429.2 
                 23651.2 
               
               
                 2.5 
                 μM 
                 30386.4 
                 30418.5 
               
               
                 5 
                 μM 
                 21663.9 
                 39843.3 
               
               
                   
               
            
           
         
       
     
     Magnetic beads conjugated with complexes of amine-SPN and its complementary sequences labeled with Cy5 in which the amine-SPN molecules and CY-5-labeled complementary sequences is at a ratio of 1:2, were incubated with peanut flour at concentrations from 0 to 500 ppm. Signals were detected and as shown in Table 14, signal decreases as peanut concentration increases. 
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Signal detection with peanut flour 
               
            
           
           
               
               
            
               
                   
                 Amine-SPN and complementary sequence labeled with Cy5 
               
            
           
           
               
               
               
            
               
                   
                 10 μl Magnetic 
                 20 μl magnetic 
               
            
           
           
               
               
               
            
               
                 peanut 
                 bead conjugates 
                 bead conjugates 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 0 
                 ppm 
                 23703.4 
                 38756.8 
               
               
                 0.5 
                 ppm 
                 27125.8 
                 38830 
               
               
                 5 
                 ppm 
                 26329.7 
                 38264.1 
               
               
                 50 
                 ppm 
                 25576.2 
                 36271 
               
               
                 500 
                 ppm 
                 19263.4 
                 33445.4 
               
               
                 2500 
                 ppm 
                 8798.1 
                 17685.8 
               
               
                   
               
            
           
         
       
     
     Example 7: Magnetic Beads Absorbance 
     To test if the same amount of beads are analyzed during target detection, the absorbance signals were analyzed in parallel with the fluorescent signal. Magnetic beads conjugated with SPN-complement complexes of amine-SPN and its complementary sequence (5nt in length) labeled with Cy5 in which the amine-SPN molecules and CY-5-labeled complementary sequences is at a ratio of 1:2, were incubated with peanut flour at concentrations from 0 to 500 ppm. Absorbance by beads was measured. The absorbance data indicates a change in absorbance when magnetic beaded conjugated to SPNs are exposed to higher concentrations of peanut flour (See Table 15). 
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 Absorbance by beads with amine-SPNs 
               
            
           
           
               
               
            
               
                   
                 Peanut 
               
            
           
           
               
               
               
               
               
               
            
               
                 Amine-SPN 
                 0 ppm 
                 0.5 ppm 
                 5 ppm 
                 50 ppm 
                 500 ppm 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                  50 nM 
                 454.3 
                 443.2 
                 438.5 
                 405.6 
                 385.9 
               
               
                 250 nM 
                 13796.8 
                 14562.2 
                 15624.6 
                 13546.4 
                 10750.2 
               
               
                 500 nM 
                 30671.6 
                 33112.6 
                 33363.3 
                 31182 
                 25944.6 
               
               
                   
               
            
           
         
       
     
     Similarly, magnetic beads conjugated with SPN-complement complexes of thiol-SPN and its complementary sequence (10 nt in length) labeled with Texas red in which the amine-SPN molecules and Texas red-labeled complementary sequences is at a ratio of 1:2, were incubated with peanut flour at concentrations from 0 to 500 ppm. Absorbance by beads was measured. As shown in Table 16, the absorption data indicates no changes in absorbance with beads conjugated to thiol-SPNs. 
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Absorbance by beads with thioL-SPNs 
               
            
           
           
               
               
            
               
                   
                 Peanut 
               
            
           
           
               
               
               
               
               
               
            
               
                 Thiol-SPN 
                 0 ppm 
                 0.5 ppm 
                 5 ppm 
                 50 ppm 
                 500 ppm 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 250 nM 
                 12357.8 
                 12397.8 
                 12087.6 
                 11863.2 
                 10599.2 
               
               
                 500 nM 
                 35442 
                 36624 
                 35181.6 
                 36071.2 
                 32547.8 
               
               
                   
               
            
           
         
       
     
     Example 8: DNA Printing on the Surface of Glass Slides 
     Optimize the Printing Protocol 
     3 complementary sequences ranging in length between 10-15 nucleotides with a 3′amine modification are used for the test. The DNA sequences are attached and printed on the surface of the glass slides using a variety of DNA loading densities and slide coatings. 
     Screen for Complementary Sequences for a Selected Aptamer 
     A microarray-based screen is used to select complementary DNA sequences that only bind the aptamer when it is not bound to the allergen protein. An aptamer (SEQ ID NO. 353 and SEQ ID NO. 307) specific to peanut allergen protein AraH1 and an aptamer (SEQ ID NO. 564) specific to nut were selected. About 40 short sequences that are complementary to different portions of each aptamer are designed based on the aptamer sequence (See Table 17). These sequences range in length between 10-20 nucleotides. The short sequences (also referred to as anchor or linker sequences) are printed on the surface of the glass slide using the printing protocol optimized previously. 
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 Short complementary sequences 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 SEQ ID NO. 
                 Complement (5′-3′) 
                   
                 SEQ ID NO. 
                 Complement (5′-3′) 
               
               
                   
               
               
                 AraH1 
                  96 
                 TCGCACATTCCGCTTCTACC 
                 P10 
                 307 
                 TAGGGAAGAGAAGGACATATG 
               
               
                   
                   
                 GGGGGGGTCGAGCTGAGTGG 
                   
                   
                 ATGTCAGTCGATGGATGTGGT 
               
               
                   
                   
                 ATGCGAATCTGTGGGTGGGC 
                   
                   
                 TGTGCTCGTCTTGACTAGTAC 
               
               
                   
                   
                 CGTAAGTCCGTGTGTGCGAA 
                   
                   
                 ATGACCACTT 
               
               
                   
               
               
                 A_1 
                 697 
                 TTCGCACACA 
                 P_1 
                 739 
                 AAGTGGTCAT 
               
               
                   
               
               
                 A_2 
                 698 
                 ACACACGGAC 
                 P_2 
                 740 
                 GTCATGTACT 
               
               
                   
               
               
                 A_3 
                 699 
                 CGGACTTACG 
                 P_3 
                 741 
                 GTACTAGTCA 
               
               
                   
               
               
                 A_4 
                 700 
                 TTACGGCCCA 
                 P_4 
                 742 
                 AGTCAAGACG 
               
               
                   
               
               
                 A_5 
                 701 
                 GCCCACCCAC 
                 P_5 
                 743 
                 AGACGAGCAC 
               
               
                   
               
               
                 A_6 
                 702 
                 CCCACAGATT 
                 P_6 
                 744 
                 AGCACAACCC 
               
               
                   
               
               
                 A_7 
                 703 
                 AGATTCGCAT 
                 P_7 
                 745 
                 AACCCACATC 
               
               
                   
               
               
                 A_8 
                 704 
                 CGCATCCACT 
                 P_8 
                 746 
                 ACATCCATCG 
               
               
                   
               
               
                 A_9 
                 705 
                 CCACTCAGCT 
                 P_9 
                 747 
                 CATCGACTGA 
               
               
                   
               
               
                 A_10 
                 706 
                 CAGCTCGACC 
                 P_10 
                 748 
                 ACTGACATCA 
               
               
                   
               
               
                 A_11 
                 707 
                 CGACCCCCCC 
                 P_11 
                 749 
                 CATCATATGT 
               
               
                   
               
               
                 A_12 
                 708 
                 CCCCCGGTAG 
                 P_12 
                 750 
                 TATGTCCTTC 
               
               
                   
               
               
                 A_13 
                 709 
                 GGTAGAAGCG 
                 P_13 
                 751 
                 CCTTCTCTTC 
               
               
                   
               
               
                 A_14 
                 710 
                 AAGCGGAATG 
                 P_14 
                 752 
                 TCTTCCCTA 
               
               
                   
               
               
                 A_15 
                 711 
                 GAATGTGCGA 
                 P_15 
                 753 
                 AAGTGGTCATGTACT 
               
               
                   
               
               
                 A_16 
                 712 
                 TTCGCACACACGGAC 
                 P_16 
                 754 
                 GTCATGTACTAGTCA 
               
               
                   
               
               
                 A_17 
                 713 
                 ACACACGGACTTACG 
                 P_17 
                 755 
                 GTACTAGTCAAGACG 
               
               
                   
               
               
                 A_18 
                 714 
                 CGGACTTACGGCCCA 
                 P_18 
                 756 
                 AGTCAAGACGAGCAC 
               
               
                   
               
               
                 A_19 
                 715 
                 TTACGGCCCACCCAC 
                 P_19 
                 757 
                 AGACGAGCACAACCC 
               
               
                   
               
               
                 A_20 
                 716 
                 GCCCACCCACAGATT 
                 P_20 
                 758 
                 AGCACAACCCACATC 
               
               
                   
               
               
                 A_21 
                 717 
                 CCCACAGATTCGCAT 
                 P_21 
                 759 
                 AACCCACATCCATCG 
               
               
                   
               
               
                 A_22 
                 718 
                 AGATTCGCATCCAT 
                 P_22 
                 760 
                 ACATCCATCGACTGA 
               
               
                   
               
               
                 A_23 
                 719 
                 CGCATCCACTCAGCT 
                 P_23 
                 761 
                 CATCGACTGACATCA 
               
               
                   
               
               
                 A_24 
                 720 
                 CCACTCAGCTCGACC 
                 P_24 
                 762 
                 ACTGACATCATATGT 
               
               
                   
               
               
                 A_25 
                 721 
                 CAGCTCGACCCCCCC 
                 P_25 
                 763 
                 CATCATATGTCCTTC 
               
               
                   
               
               
                 A_26 
                 722 
                 CGACCCCCCCGGTAG 
                 P_26 
                 764 
                 TATGTCCTTCTCTTC 
               
               
                   
               
               
                 A_27 
                 723 
                 CCCCCGGTAGAAGCG 
                 P_27 
                 765 
                 CCTTCTCTTCCCTA 
               
               
                   
               
               
                 A_28 
                 724 
                 GGTAGAAGCGGAATG 
                 P_28 
                 766 
                 AAGTGGTCATGTACTAGTCA 
               
               
                   
               
               
                 A_29 
                 725 
                 AAGCGGAATGTGCGA 
                 P_29 
                 767 
                 GTCATGTACTAGTCAAGACG 
               
               
                   
               
               
                 A_30 
                 726 
                 TTCGC ACACACGGACTTACG 
                 P_30 
                 768 
                 GTACTAGTCAAGACGAGAC 
               
               
                   
               
               
                 A_31 
                 727 
                 ACACACGGACTTACGGCCCA 
                 P_31 
                 769 
                 AGTCAAGACGAGCACAACCC 
               
               
                   
               
               
                 A_32 
                 728 
                 CGGACTTACGGCCCACCCAC 
                 P_32 
                 770 
                 AGACGAGCACAACCCACATC 
               
               
                   
               
               
                 A_33 
                 729 
                 TTACGGCCCACCCACAGATT 
                 P_33 
                 771 
                 AGCACAACCCACATCCATCG 
               
               
                   
               
               
                 A_34 
                 730 
                 GCCCACCCACAGATTCGCAT 
                 P_34 
                 772 
                 AACCCACATCCATCGACTGA 
               
               
                   
               
               
                 A_35 
                 731 
                 CCCACAGATTCGCATCCACT 
                 P_35 
                 773 
                 ACATCCATCGACTGACATCA 
               
               
                   
               
               
                 A_36 
                 732 
                 AGATTCGCATCCACTCAGCT 
                 P_36 
                 774 
                 CATCGACTGACATCATATGT 
               
               
                   
               
               
                 A_37 
                 733 
                 CGCATCCACTCAGCTCGACC 
                 P_37 
                 775 
                 ACTGACATCATATGTCCTTC 
               
               
                   
               
               
                 A_38 
                 734 
                 CCACTCAGCTCGACCCCCCC 
                 P_38 
                 776 
                 CATCATATGTCCTTC TCTTC 
               
               
                   
               
               
                 A_39 
                 735 
                 CAGCTCGACCCCCCCGGTAG 
                 P_39 
                 777 
                 TATGTCCTTCTCTTCCCTA 
               
               
                   
               
               
                 A_40 
                 736 
                 CGACCCCCCCGGTAGAAGCG 
                 P_40 
                   
                   
               
               
                   
               
               
                 A_41 
                 737 
                 CCCCCGGTAGAAGCGGAATG 
                 P_41 
                   
                   
               
               
                   
               
               
                 A_42 
                 738 
                 GGTAGAAGCGGAATGTGCGA 
                 P_42 
               
               
                   
               
            
           
         
       
     
     Each of 16-array slides is coated with 3 copies of the 50 sequences, giving a total of 150 sequences per array (see chip layout in Table 18) using the printing protocol optimized previously. The binding of the short sequences to its target aptamer is tested using either an attachment or detachment protocol detailed below. The aptamer is labeled on the 5′ end with a fluorescent dye Cy5 which is used to quantify the aptamer bound to the plate. The allergenic protein is prepared into a serial dilution of five different concentrations (e.g., 0 ppm, 1 ppm, 10 ppm, 100 ppm, and 1000 ppm). The output of the experiment is relative Cy5 reads from the protein curve as well as the negative controls. Table 17 shows an example of the screen chip layout. The screen consists of three 16-array slides. Each array is designed to have 3 copies of the 50 sequences (150 samples total). For each experimental slide, a 5-point protein curve (×3) and a buffer control are included (a full 16 array slide). 
                     TABLE 18                  Screen chip layout                         Slide    Treat-           No.   ment   Slide setup                                                     1   Attach-   0 ppm   1 ppm   10 ppm   100 ppm   1000 ppm               ment   0 ppm   1 ppm   10 ppm   100 ppm   1000 ppm               protocol   0 ppm   1 ppm   10 ppm   100 ppm   1000 ppm   buffer           (tripli -                                   cates)                               2   Detach-   0 ppm   1 ppm   10 ppm   100 ppm   1000 ppm               ment   0 ppm   1 ppm   10 ppm   100 ppm   1000 ppm               protocol   0 ppm   1 ppm   10 ppm   100 ppm   1000 ppm   buffer           (tripli-                                   cates)                               3   Controls   0 ppm   1 ppm   10 ppm   100 ppm   1000 ppm               (dupli-   0 ppm   1 ppm   10 ppm   100 ppm   1000 ppm               cates)                                       buffer   buffer   aptamer   aptamer   aptamer   aptamer                    
Attachment Assay
 
     For the attachment protocol, a fixed concentration of aptamer is mixed with increasing concentrations of the allergen protein for 1 min to allow the aptamer to bind to the allergen protein. The protein-aptamer mixture is then added to the wells of the plate and incubated for 1 min at room temperature, washed, and measured for fluorescence. The controls for the experiment include determining fluorescence of the microarray wells with either only the aptamer or protein not previously incubated with the aptamer, as well as fluorescence with only the short sequences present. Aptamer that is protein-free attaches to the short sequences immobilized on the plate and contributes to the fluorescence reading. Protein-bound aptamer does not attach to the plate and is washed away. If the short immobilized DNA sequence only binds the protein-free aptamer, decreasing fluorescence signal is observed with an increasing concentration of the protein, as the higher the protein concentration the less protein-free aptamer is available to bind to the short sequence on the microarray. If the short sequence cannot differentiate between protein-bound and unbound aptamer, a similar fluorescence intensity is observed regardless of the concentration of the protein. 
     Detachment Assay 
     For the detachment protocol, the aptamer is added to the wells of the plate and incubated for 1 min at room temperature, washed, and measured for fluorescence. Protein is added to the plate at five different concentrations, incubated for 1 min, washed, and measured for fluorescence again. The controls of the experiment are the same as described for the attachment protocol. If the short sequence only binds the protein-free aptamer, a drop in fluorescence signal is observed when protein is added. The magnitude of the drop increases with an increasing concentration of the protein, as the higher the protein concentration the more aptamers detach from the short immobilized sequence. If the short sequence cannot differentiate between protein-bound and unbound aptamer, a similar fluorescence intensity is observed regardless of the concentration of the protein. 
     The top 3 short sequences are selected based on the screen results and validated using a second microarray. Each one of the 3 sequences is printed in 50 spots per array, giving a total of 150 sequences. The binding to the short sequences is tested using either an attachment or detachment protocol as described above. The validation assay incorporates control proteins and control test matrices (see chip layout in Table 18). Relative Cy5 reads from different protein concentrations as well as the negative controls are compared. The best candidate meets the following criteria: (a) specific binding to the corresponding aptamer, (b) easy detachment from the bound aptamer in the presence of the allergenic protein, and (c) minimal background/non-specific binding. The selected complementary sequence is used to form SPN-complement complexes. 
     Table 19 shows the chip layout for the validation assay. A set of 12, 16-array slides are used for the attachment assay and another 12 are used for the detachment assay. For each slide, a 5-point curve (×3) and a control are included (a full 16 array slide). Each spot of the 16 array slides contains 50 copies of the 3 candidates selected from the screen (150 samples total). 
     
       
         
           
               
             
               
                 TABLE 19 
               
             
            
               
                   
               
               
                 Validation Chip layout 
               
            
           
           
               
               
               
            
               
                 Slide No. 
                 Category 
                 Slide setup 
               
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 Test 
                 protein curve + aptamer in buffer 
               
               
                 2 
                 Test 
                 protein curve + aptamer in food 1 
               
               
                 3 
                 Test 
                 protein curve + aptamer in food 2 
               
               
                 4 
                 Test 
                 protein curve + aptamer in food 3 
               
               
                 5 
                 Control 
                 control protein + aptamer curve 1 
               
               
                 6 
                 Control 
                 control protein + aptamer curve 2 
               
               
                 7 
                 Control 
                 control protein + aptamer curve 3 
               
               
                 8 
                 Control 
                 aptamer, no protein 
               
               
                 9 
                 Control 
                 no aptamer in buffer 
               
               
                 10 
                 Control 
                 no aptamer in food 1 
               
               
                 11 
                 Control 
                 no aptamer in food 2 
               
               
                 12 
                 Control 
                 no aptamer in food 3 
               
               
                   
               
            
           
         
       
     
     Example 9: Validation of Detection Sensitivity of SPN-Magnetic Beads Conjugates 
     As discussed in Example 1, 5′ Thiol modified short complementary sequences were conjugated to magnetic beads and aptamer specific to peanut allergen (SEQ ID NO. 96: 5′ (Texas Red) TCGCACATTCCGCTTCTACCGGGGGGTCGAGCTGAGTGGATGCGAATCTGTGGG TGGGCCGTAAGTCCGTGTGTGCGAA3′) was hybrided with the short linker nucleic acid molecules. The aptamer was labeled with Texas red at the 5′ end. The short linker sequence is AAAAATCAAGTGGTC with 5′ Thiol modification (SEQ ID NO. 778) 
     The SPN-magnetic bead conjugates were used as detection agents to validate the detection sensitivity in detection assays. Peanut was diluted in either buffer or cookie at 0 ppm, 5 ppm, 50 ppm and 500 ppm, respectively. The prepared peanut samples were reacted with SPN-magnetic bead conjugates (loaded in 96-well microplate) and signals were read using lab-grade LED based reader after incubation and wash. Under bench-top reaction conditions, peanut present either in buffer or in cookie can be detected at a low ppm using SPN-magnetic bead conjugates. The recorded signal indicates that the fluorescent signal is correlatively reduced when the concentration of peanut increases. At the concentration of 50 ppm (peanut commodity), a significant fluorescent signal reduction is recorded in buffer ( FIG. 7A ) and cookie ( FIG. 7B ). This observation confirms the 50 ppm detection sensitivity as shown in previous assays. In a similar assay, when peanut was added to other food samples, a significant signal reduction is seen at 50 ppm peanut ( FIG. 8B ). Comparison of food samples with peanut and those containing no peanut, at least 25% of signal reduction is observed in all samples compared ( FIG. 8A ). Food samples were chose based on their general concerns with allergic concerns. For example, cookie, cereal, chocolate and ice cream are commonly consumed; Blue frosting is high food coloring; Pizza is highly processed food which is hard to detect the presence of allergens using available detection assays such as the ELISA assay; and Ranch dressing is high in fat. This selection covers a broad range of matrices. These observations suggest that assays using SPN-magnetic bead conjugates can achieve a 50 ppm sensitivity with all food matrices being tested. 
     In order to further confirm the sensitivity of the SPN-magnetic bead conjugates based assay, over 20 different food matrices were spiked with peanut and tested for signal detection and its sensitivity. The 50 ppm sensitivity was confirmed on representative foods from each category as shown in Table 20. 
     
       
         
           
               
             
               
                 TABLE 20 
               
             
            
               
                   
               
               
                 The detection sensitivity of SPN-magnetic bead conjugates 
               
            
           
           
               
               
            
               
                   
                 Fluorescent signal reduction 
               
            
           
           
               
               
               
               
            
               
                 Food category 
                 Food 
                 0 ppm peanut 
                 50 ppm peanut 
               
               
                   
               
               
                 Baked goods 
                 cookie 
                 100% 
                 71% 
               
               
                   
                 Red Velvet Cake 
                 100% 
                 86% 
               
               
                   
                 Oatmeal Cookie 
                 100% 
                 68% 
               
               
                   
                 Waffer 
                 100% 
                 47% 
               
               
                 Sauses, dressings 
                 Dressing 
                 100% 
                 67% 
               
               
                 and Marinades 
                 salsa 
                 100% 
                 61% 
               
               
                   
                 Tomato sauce 
                 100% 
                 81% 
               
               
                   
                 Hummus 
                 100% 
                 77% 
               
               
                 Entrees 
                 Oatmeal 
                 100% 
                 73% 
               
               
                   
                 Sausage 
                 100% 
                 67% 
               
               
                   
                 Bread 
                 100% 
                 82% 
               
               
                   
                 French Fries 
                 100% 
                 37% 
               
               
                   
                 Pizza 
                 100% 
                 76% 
               
               
                   
                 Chicken 
                 100% 
                 60% 
               
               
                 Desserts 
                 Vanilia Pudding 
                 100% 
                 68% 
               
               
                   
                 Vanilla Oreo 
                 100% 
                 79% 
               
               
                   
                 Meringue 
                 100% 
                 64% 
               
               
                   
                 Blue Frosting 
                 100% 
                 64% 
               
               
                   
                 Sorbet 
                 100% 
                 21% 
               
               
                   
                 Ice Cream 
                 100% 
                 79% 
               
               
                   
                 Blueberry Yogurt 
                 100% 
                 82% 
               
               
                   
                 Grape Jelly 
                 100% 
                 41% 
               
               
                   
               
            
           
         
       
     
     The detection sensitivity of SPN-magnetic bead conjugates was further confirmed using different fluorescent signal readers. The signal reading was recorded and analyzed using a lab-grade LED based reader as well as a designed optical sensor. When the detection assay was carried out using Data shown in Table 21 indicate that the detection sensitivity is not affected when using different signal readers. The designed optical sensor is comparable to the laboratory-standard microplate reader (e.g.,  FIGS. 8A and 8B ; and Table 20) and can differentiate between 0 ppm and 50 ppm peanut in food with 95% confidence. 
     
       
         
           
               
             
               
                 TABLE 21 
               
             
            
               
                   
               
               
                 The detection sensitivity of SPN-magnetic bead conjugates 
               
            
           
           
               
               
               
            
               
                   
                 Fluorescent signal reduction 
                   
               
            
           
           
               
               
               
               
            
               
                   
                 Food 
                 0 ppm peanut 
                 50 ppm peanut 
               
               
                   
                   
               
               
                   
                 Cookie 
                 100% 
                 62% 
               
               
                   
                 Sorbert 
                 100% 
                 28% 
               
               
                   
                 Frosting 
                 100% 
                 72% 
               
               
                   
                 Dressing 
                 100% 
                 67% 
               
               
                   
                 Pizza 
                 100% 
                 72% 
               
               
                   
                 Cracker 
                 100% 
                 53% 
               
               
                   
                 Cookie 
                 100% 
                 65% 
               
               
                   
                 Ice cream 
                 100% 
                 65% 
               
               
                   
                 Biscuit 
                 100% 
                 62% 
               
               
                   
                 Pretzel 
                 100% 
                 57% 
               
               
                   
                 Waffer 
                 100% 
                 47% 
               
               
                   
                 Poptart 
                 100% 
                 45% 
               
               
                   
                   
               
            
           
         
       
     
     Example 10: Detection Assays with Nucleic Acid Coated Chips 
     Chips were coated with short complementary DNA sequences that bind the aptamer only when it is not bound to the allergen protein. These short nucleic acid anchor sequences specific to the aptamers (SEQ ID NO. 307, SEQ ID NO. 404 and SEQ ID NO. 304) which bind to peanut allergen protein were used to coat the glass. These anchor sequences are about 10 nt in length. The short anchor sequences are further modified to contain 5 (polyA) at one end to pull the sequence away from the surface. A 6 carbon or 12 Carbon linker is further added to the sequences to pull them further away from the surface. An amine attachment is added to print the sequences to the surface of the chip. The sequences and modification of the anchor/linker DNA molecules are listed in Table 22. Prepared peanut containing samples were incubated with a fixed concentration of SPNs specific to the peanut allergen and filtered to remove unbound food particles. The aptamers were labeled with Cy5 at the 5′ end. The mixture was incubated with the chip coated with short linker/anchor sequences. After incubation and wash, the fluorescent reading was recorded using a lab grade LED based reader, as well as the designed optical sensor. The anchor sequences that generate the most specific and sensitive signals are selected for further validation. 
     
       
         
           
               
             
               
                 TABLE 22 
               
             
            
               
                   
               
               
                 Sequences used to coat the solid surface 
               
               
                 (chip) and their modifications 
               
            
           
           
               
               
               
               
            
               
                 Name 
                 Sequence 
                 Modification 
                 SEQ ID NO. 
               
               
                   
               
               
                 AP_1 
                 /5AmMC6/TTCGCACACA 
                 5′ 6CAmine 
                 697 
               
               
                   
               
               
                 AP_2 
                 /5AmMC6/ACACACGGAC 
                 5′ 6CAmine 
                 698 
               
               
                   
               
               
                 AP_3 
                 /5AmMC6/CGGACTTACG 
                 5′ 6CAmine 
                 699 
               
               
                   
               
               
                 AP_4 
                 /5AmMC6/TTACGGCCCA 
                 5′ 6CAmine 
                 700 
               
               
                   
               
               
                 AP_5 
                 /5AmMC6/GCCCACCCAC 
                 5′ 6CAmine 
                 701 
               
               
                   
               
               
                 AP_6 
                 /5AmMC6/CCCACAGATT 
                 5′ 6CAmine 
                 702 
               
               
                   
               
               
                 AP_7 
                 /5AmMC6/AGATTCGCAT 
                 5′ 6CAmine 
                 703 
               
               
                   
               
               
                 AP_8 
                 /5AmMC6/CGCATCCACT 
                 5′ 6CAmine 
                 733 
               
               
                   
               
               
                 AP_9 
                 /5AmMC6/CCACTCAGCT 
                 5′ 6CAmine 
                 705 
               
               
                   
               
               
                 AP_10 
                 /5AmMC6/CAGCTCGACC 
                 5′ 6CAmine 
                 706 
               
               
                   
               
               
                 AP_11 
                 /5AmMC6/CGACCCCCCC 
                 5′ 6CAmine 
                 707 
               
               
                   
               
               
                 AP_12 
                 /5AmMC6/CCCCCGGTAG 
                 5′ 6CAmine 
                 708 
               
               
                   
               
               
                 AP_13 
                 /5AmMC6/GGTAGAAGCG 
                 5′ 6CAmine 
                 709 
               
               
                   
               
               
                 AP_14 
                 /5AmMC6/AAGCGGAATG 
                 5′ 6CAmine 
                 710 
               
               
                   
               
               
                 AP_15 
                 /5AmMC6/GAATGTGCGA 
                 5′ 6CAmine 
                 711 
               
               
                   
               
               
                 3_1 
                 /5AmMC6/aaaaaTCAAGTGGTC 
                 5′ 6CAmine 
                 778 
               
               
                   
               
               
                 3_2 
                 /5AmMC6/aaaaaTGGTCATGTA 
                 5′ 6CAmine 
                 779 
               
               
                   
               
               
                 3_3 
                 /5AmMC6/aaaaaTGTACTAGT 
                 5′ 6CAmine 
                 780 
               
               
                   
               
               
                 3_4 
                 /5AmMC6/aaaaaTACTAGTCAA 
                 5′ 6CAmine 
                 781 
               
               
                   
               
               
                 5_1 
                 /5AmMC6/aaaaaATCAT ATGTC 
                 5′ 6CAmine 
                 782 
               
               
                   
               
               
                 5_2 
                 /5AmMC6/aaaaaATGTCCTTCT 
                 5′ 6CAmine 
                 783 
               
               
                   
               
               
                 5_3 
                 /5AmMC6/aaaaaCTTCTCTTCC 
                 5′ 6CAmine 
                 784 
               
               
                   
               
               
                 5_4 
                 /5AmMC6/aaaaaCTCTTCCCTA 
                 5′ 6CAmine 
                 785 
               
               
                   
               
               
                 P1_10 
                 /5AmMC6/aaaaaCATCGACTGACA 
                 5′ 6CAmine 
                 786 
               
               
                   
               
               
                 P2_8 
                 /5AmMC6/aaaaaCCATCGATGC 
                 5′ 6CAmine 
                 787 
               
               
                   
               
               
                 P2_18 
                 /5AmMC6/aaaaaCTACACCCACATC 
                 5′ 6CAmine 
                 788 
               
               
                   
               
               
                 AP_1_PA 
                 /5AmMC6/aaaaaTTCGCACACA 
                 5′ 6CAmine 
                 789 
               
               
                   
               
               
                 AP_2_PA 
                 /5AmMC6/aaaaaACACACGGAC 
                 5′ 6CAmine 
                 790 
               
               
                   
               
               
                 AP_3_PA 
                 /5AmMC6/aaaaaCGGACTTACG 
                 5′ 6CAmine 
                 791 
               
               
                   
               
               
                 AP_4_PA 
                 /5AmMC6/aaaaaTTACGGCCCA 
                 5′ 6CAmine 
                 792 
               
               
                   
               
               
                 AP_5_PA 
                 /5AmMC6/aaaaaGCCCACCCAC 
                 5′ 6CAmine 
                 793 
               
               
                   
               
               
                 AP_6_PA 
                 /5AmMC6/aaaaaCCCACAGATT 
                 5′ 6CAmine 
                 794 
               
               
                   
               
               
                 AP_7_PA 
                 /5AmMC6/aaaaaAGATTCGCAT 
                 5′ 6CAmine 
                 795 
               
               
                   
               
               
                 AP_8_PA 
                 /5AmMC6/aaaaaCGCATCCACT 
                 5′ 6CAmine 
                 796 
               
               
                   
               
               
                 AP_9_PA 
                 /5AmMC6/aaaaaCCACTCAGCT 
                 5′ 6CAmine 
                 797 
               
               
                   
               
               
                 AP_10_PA 
                 /5AmMC6/aaaaaCAGCTCGACC 
                 5′ 6CAmine 
                 798 
               
               
                   
               
               
                 AP_11_PA 
                 /5AmMC6/aaaaaCGACCCCCCC 
                 5′ 6CAmine 
                 799 
               
               
                   
               
               
                 AP_12_PA 
                 /5AmMC6/aaaaaCCCCCGGTAG 
                 5′ 6CAmine 
                 800 
               
               
                   
               
               
                 AP_13_PA 
                 /5AmMC6/aaaaaGGTAGAAGCG 
                 5′ 6CAmine 
                 801 
               
               
                   
               
               
                 AP_14_PA 
                 /5AmMC6/aaaaaAAGCGGAATG 
                 5′ 6CAmine 
                 802 
               
               
                   
               
               
                 AP_15_PA 
                 /5AmMC6/aaaaaGAATGTGCGA 
                 5′ 6CAmine 
                 803 
               
               
                   
               
            
           
         
       
     
     Example 11: Sensitivity of the Detection Assay Based on Nucleic Acid Coated Chip 
     Peanut detection using nucleic acid coated chips was evaluated in different detection assays and with different food matrices. The sensitivity of the detection assay based on nucleic acid coated chips was validated and confirmed. In this study, the anchor/linker sequence (5′ 12C-Amine) AAAAATTCGCACACA (SEQ ID NO. 789) was printed on the surface of the chip. As shown in  FIG. 9A , peanut diluted in buffer at various concentrations was incubated with peanut specific SPN (SEQ ID NO. 96: 5′ (Cy5) TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGTGGG TGGGCCGTAAGTCCGTGTGTGCGAA3′) and run through the DNA coated solid surface of the chip, the fluorescent signal recorded in nucleic acid coated chip based detection assay is correlated with the increased peanut concentration in buffer. The signal change can be detected as a low concentration of peanut (e.g., 5 ppm). Similarly, peanut diluted spiked in shortbread at various concentrations was incubated with peanut specific SPN and run through the DNA coated solid surface of the chip, the fluorescent signal recorded in nucleic acid coated chip based detection assay is correlated with the increased peanut concentration in buffer ( FIG. 9A ). The data also indicate that the SPN detection sensitivity is food dependent. In food matrices, the sensitivity is decreased as compared in simple buffer samples ( FIGS. 9A and 9B ). 
     Detection assays with nucleic acid coated chip were tested both on a designed reaction system (e.g., the rig), and the benchtop reaction and LED read. More than 20 food samples were tested and the detection sensitivity of the SPN in solid surface based assay is repeatable using different solid surfaces (see, e.g.,  FIGS. 10A and 10B ). The designed reaction system contains a test cartridge the hold the extraction buffer and wash buffer (e.g., HEPES buffer and TGK T-Buffer). SPN molecules at various concentrations (5 nM, 10 nM, or 50 nM) are pre-loaded in the extraction buffer. The wash buffer does not have the SPN. The cartridge also holds the DNA coated chip inside a reaction chamber within the cartridge. A driving system is also provided to activate the cartridge, driving the homogenization of the food and controlling the flow of the sample during the reaction. It also has an optical system attached to measure the detection system. In both reaction settings, a comparable detection sensitivity can be achieved ( FIGS. 10A and 10B ). The same sensitivity of 12.5 ppm peanut protein (equal to 50 ppm peanut commodity) is also retained in diverse chocolate matrices as tested on the rig with nucleic acid coated chips ( FIG. 10C ). 
     Example 12. Comparison of Detection Sensitivity Between SPN-Magnetic Bead Conjugates and Nucleic Acid Coated Solid Surface (Chip) 
     Detection assays using SPN-magnetic bead conjugates and nucleic acid coated chips were validated in various conditions using a variety of different food samples. The detection sensitivity is confirmed in different conditions. For assays using SPN-magnetic bead conjugates, food samples without or with 50 ppm peanut were prepared using GentleMACS homogenizer and centrifuged. The processed samples were then incubated with SPN-magnetic bead conjugates and fluorescent signals were recorded using lab-grade LED based reader. The 50 ppm sensitivity was confirmed in 20 food samples tested ( FIG. 11A ). In a parallel study, food samples without or with 50 ppm peanut were prepared using rotor-dependent homogenizer and homogenized samples were filtered using 0.2 mm PES filter. The processed samples were then incubated with SPN-magnetic bead conjugates and fluorescent signals were recorded using lab-grade LED based reader. The 50 ppm sensitivity was confirmed in 5 food samples tested ( FIG. 11C ). 
     Detection assays were also performed using nucleic acid coated chips as described in Example 10. Food samples without or with 50 ppm peanut were prepared using GentleMACS homogenizer and centrifuged, or alternatively prepared using rotor-dependent homogenizer and filtered using 0.1 mm PES filter. The processed samples were then incubated with a fixed concentration of aptamer specific to peanut allergen. The mixed samples were added to the nucleic acid coated chips. After incubation and wash, the fluorescent signals were recorded using designed optical sensors. The 50 ppm sensitivity was achieved in different food samples ( FIG. 11B ) and was further confirmed in 9 food samples tested ( FIG. 11D ). 
     The sensitivity of SPN-magnetic beads and nucleic acid coated chips was also tested in different reaction settings such as standard bench-top reaction chamber and a designed reaction chamber (e.g., the rig). The rig is a benchtop fixture that includes a motor, syringe pump and valving the enables the homogenization of the food sample, filtering through a filter bed and running the sample over the reaction chamber in which the DNA coated chip is located. The rig enables controlled washing. The detailed conditions are listed in Table 23. In all conditions tested, the 50 ppm sensitivity can be achieved. 
     
       
         
           
               
             
               
                 TABLE 23 
               
             
            
               
                   
               
               
                 Validation of assay sensitivity 
               
            
           
           
               
               
            
               
                   
                 Fluorescent signal 
               
               
                   
                 reduction 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 0 ppm 
                 50 ppm 
               
               
                 Assay 
                 Assay conditions 
                 Food 
                 peanut 
                 peanut 
               
               
                   
               
               
                 Magnetic 
                 GentleMACS homogenizer 
                 Lemon 
                 100% 
                 22% 
               
               
                 beads 
                 and centrifugation; Lab- 
                 sobert 
               
               
                 (N = 3) 
                 standard bench-top 
                 Shortbread 
                 100% 
                 67% 
               
               
                   
                 reaction; designed optical 
                 cooike 
               
               
                   
                 sensor 
                 Blue 
                 100% 
                 79% 
               
               
                   
                   
                 frosting 
               
               
                   
                   
                 Pizza 
                 100% 
                 70% 
               
               
                   
                   
                 Dressing 
                 100% 
                 84% 
               
               
                 Nucleic 
                 Designed Rotor- 
                 Lemon 
                 100% 
                 76% 
               
               
                 acid solid 
                 homogenizer and 1 mm 
                 sobert 
               
               
                 surface 
                 PES filter; Lab-standard 
                 Shortbread 
                 100% 
                 69% 
               
               
                 (N = 4) 
                 bench-top reaction; 
                 cooike 
               
               
                   
                 designed optical sensor 
                 Blue 
                 100% 
                 67% 
               
               
                   
                   
                 frosting 
               
               
                   
                   
                 Pizza 
                 100% 
                 76% 
               
               
                   
                   
                 Dressing 
                 100% 
                 73% 
               
               
                 Magnetic 
                 Designed Rotor- 
                 Lemon 
                 100% 
                 36% 
               
               
                 beads 
                 homogenizer and bulk 
                 sobert 
               
               
                 (N = 2) 
                 filters; designed rig 
                 Shortbread 
                 100% 
                 65% 
               
               
                   
                 reaction chamber; designed 
                 cooike 
               
               
                   
                 optical sensor 
                 Blue 
                 100% 
                 72% 
               
               
                   
                   
                 frosting 
               
               
                   
                   
                 Pizza 
                 100% 
                 51% 
               
               
                   
                   
                 Dressing 
                 100% 
                  0% 
               
               
                 Nucleic 
                 Designed Rotor- 
                 Lemon 
                 100% 
                 36% 
               
               
                 acid solid 
                 homogenizer and 1 mm 
                 sobert 
               
               
                 surface 
                 PES filter; designed rig 
                 Shortbread 
                 100% 
                 50% 
               
               
                 (N = 3) 
                 reaction chamber; designed 
                 cooike 
               
               
                   
                 optical sensor 
                 Blue 
                 100% 
                 72% 
               
               
                   
                   
                 frosting 
               
               
                   
                   
                 Pizza 
                 100% 
                 56% 
               
               
                   
                   
                 Dressing 
                 100% 
                 53% 
               
               
                   
               
            
           
         
       
     
     Example 13: Optimizing Detection Assays on Solid Surface 
     Limited Reaction Buffer 
     The detection assay run on nucleic acid coated solid surface (e.g., chip) was optimized in several aspects and its sensitivity was validated in various reaction conditions. The effect of buffer volume on the detection sensitivity was tested in one study. In the nucleic acid chip assay, when the reaction volume is decreased by 50% (2.5 ml) and only half of the sample size (0.25 g per sample) was used, the detection result indicates that limited buffer volume and reduced sample size has minimal effect on the detection sensitivity of SPNs and solid surface assays ( FIG. 12 ). 
     Internal Control 
     To reduce background recording and signals from non-specific bindings, control signals to subtract these non-specific readings are important to optimize the assay. An internal control signal was recorded to correct signal measurement. The two control panels as shown in  FIG. 13A  contain the sequence GAAAAGTGCTCATCTGTGAACTCTAT (SEQ ID NO. 804) that has CY5 bound to on end and amine on the other side. The sequences are printed on the surface of the chip at various patterns (not shown) but keep the similar relevant positions to the detection area where DNA anchor/linker sequences specific to SPNs are printed (as the diagram shown in  FIG. 13A ). In general, control areas are located at various locations outside of the detection area on the chip. After sample incubation and wash, fluorescence signals from the detection area and control areas are read and analysed to indicate the concentration of allergen detected in the sample. 
     Concentration of MgCl2 
     Fluorescent signal is likely be affected by many factors such as salts and buffer concentration, etc. The concentration of MgCl2 in reaction buffer is to optimize the detection assay to achieve high-sensitive and intense signal. 0 mM to 120 mM MgCl2 was tested and MgCl2 was added either to extraction buffer or after food sample extraction and filtration. The results suggest that addition of MgCl2 to extraction buffer completely eliminates sensitivity. The MgCl2 concentration needs to be tightly controlled between 25 mM and 50 mM. Lower or higher concentration of MgCl2 affects both sensitivity and signal intensity ( FIGS. 14A, 14B and 14C ) 
     Optimization of Detection Assay on the Rig 
     In addition to internal control, various parameters that affect the efficiency of detection assay were optimized including the steps of pre-blocking the DNA coated chips, decreasing reaction time and washing. It is found that DNA-chip, the solid surface can be effectively blocked by BSA, which reduce non-specific signal. During the process of assaying, the reaction time can be decreased from 6 minutes to about 64 seconds. Optimized steps include removing air bubbles for both the reaction and wash, decreasing incubation time of food sample and SPNs, transitioning to a slow flow of incubation, increasing volume of reaction to flow over the chip and increasing flow rate of solution to accommodate the volume in 30 seconds. One example of the optimized reaction step and time is shown in Table 24. 
     
       
         
           
               
             
               
                 TABLE 24 
               
             
            
               
                   
               
               
                 Optimization of reaction on the rig (solid surface) 
               
            
           
           
               
               
               
               
            
               
                   
                 Step 
                 Time 
               
               
                   
                   
               
            
           
           
               
               
               
               
            
               
                 Sample 
                 Homogenization of food in 5 ml of buffer 
                 40 
                 Sec 
               
               
                 process 
                 with 20 nM SPN; Filtration 1 micron FES filter 
               
               
                 Reaction 
                 Prime Channels and remove BSA 
                 4 
                 Sec 
               
               
                 process 
                 Adding 500 ul of food and SPN filtrate 
                 30 
                 Sec 
               
               
                   
                 Wash 500 ul 
                 20 
                 Sec 
               
               
                   
                 Air dry 
                 4 
                 Sec 
               
               
                   
                 Read 
                 1 
                 Sec 
               
               
                   
               
            
           
         
       
     
     In this protocol of detection reaction within 64 seconds, the assay sensitivity is retained ( FIG. 15 ). 
     Filtration 
     Different filters were tested and compared for efficiency in removing food particles and its effect on signal read. Three different filters: fish/netting, cotton/glass, and cotton/netting were tested and compared in 6 different food matrices spiked with peanut (Table 25). Dependent of food matrices, a different combination of filters may be used to increase allergen extraction from the sample; and to deplete other components such as fat and other particles. 
     
       
         
           
               
             
               
                 TABLE 25 
               
             
            
               
                   
               
               
                 Filter comparison 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 short- 
                   
                   
                   
                 Ranch 
                   
               
               
                   
                 Bread 
                 Blue 
                 White 
                 Bagle- 
                 Dressing 
                   
               
               
                   
                 Cookie 
                 Frosting 
                 Frosting 
                 Bites 
                 (wishbone) 
                 Sorbert 
               
               
                   
               
               
                 Cotton/glass 
                 100% 
                 100% 
                 100% 
                 100% 
                 100% 
                 100% 
               
               
                 0 ppm peanut 
                 100% 
                 100% 
                 100% 
                 100% 
                 100% 
                 100% 
               
               
                 Cotton/glass 
                  59% 
                  77% 
                  16% 
                  63% 
                  60% 
                  85% 
               
               
                 50 ppm  
                  54% 
                  59% 
                  35% 
                  67% 
                  47% 
                  51% 
               
               
                 peanut 
                   
                   
                   
                   
                   
                   
               
               
                 Fish/netting 
                 100% 
                 100% 
                 100% 
                 100% 
                 100% 
                 100% 
               
               
                 0 ppm peanut 
                 100% 
                 100% 
                 100% 
                 100% 
                 100% 
                 100% 
               
               
                 Fish/netting 
                 / 
                  76% 
                  95% 
                  72% 
                  55% 
                  85% 
               
               
                 50 ppm  
                  73% 
                  54% 
                  53% 
                  83% 
                 / 
                  75% 
               
               
                 peanut 
                   
                   
                   
                   
                   
                   
               
               
                 Cotton/netting 
                 100% 
                 100% 
                 / 
                 100% 
                 100% 
                  0% 
               
               
                 0 ppm peanut 
                 100% 
                 100% 
                 / 
                 100% 
                 100% 
                  0% 
               
               
                 Cotton/netting 
                  42% 
                  49% 
                 / 
                  9% 
                  59% 
                  0% 
               
               
                 50 ppm  
                  33% 
                  60% 
                 / 
                  79% 
                  73% 
                  0% 
               
               
                 peanut 
               
               
                   
               
            
           
         
       
     
     Example 14: Stability of Detection Agents and Other Agents for Detection Assays 
     Components used for the present detection assay need to be stable at ambient temperature. Examples of agents and other parts employed to run an allergen assay, include extraction buffer, wash buffer, Flagged-SPN and DNA chips. The stability test indicates that fluorescent labeled SPN (e.g., Texas-Red labeled SPN) is stable and its activity remains at least for one month at various temperatures. (Table 26) as measured with fluorescent polarization (FP). After 9 month storage, significant fluorescent signal can still be detected ( FIGS. 16A and 16B ). Buffers used for the detection assay can remain stable for at least 9 month ( FIGS. 16C and 16D ). 
     
       
         
           
               
             
               
                 TABLE 26 
               
             
            
               
                   
               
               
                 Stability of Texas-red labeled SPN activity at various temperatures 
               
            
           
           
               
               
            
               
                   
                 Delta signal (mP) 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 Day 0 
                 Day 1 
                 Day 2 
                 Day 4 
                 Week 2 
                 Week 3 
                 Week 4 
               
               
                   
               
            
           
           
               
               
            
               
                   
                 4° C. 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 5000 
                 152 
                 153 
                 143 
                 153 
                 154 
                 148 
                 128 
               
               
                 500 
                 114 
                 109 
                 108 
                 105 
                 106 
                 92 
                 75 
               
               
                 50 
                 36 
                 27 
                 22 
                 37 
                 31 
                 24 
                 21 
               
               
                 5 
                 4 
                 7 
                 (1) 
                 8 
                 6 
                 8 
                 8 
               
            
           
           
               
               
            
               
                   
                 25° C. 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 5000 
                 152 
                 151 
                 148 
                 149 
                 164 
                 158 
                 141 
               
               
                 500 
                 114 
                 99 
                 98 
                 107 
                 115 
                 104 
                 81 
               
               
                 50 
                 36 
                 26 
                 20 
                 35 
                 35 
                 27 
                 21 
               
               
                 5 
                 4 
                 2 
                 2 
                 12 
                 4 
                 6 
                 4 
               
            
           
           
               
               
            
               
                   
                 42° C. 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 5000 
                 152 
                 147 
                 136 
                 152 
                 161 
                 190 
                 160 
               
               
                 500 
                 114 
                 104 
                 119 
                 109 
                 119 
                 121 
                 103 
               
               
                 50 
                 36 
                 22 
                 31 
                 33 
                 34 
                 34 
                 26 
               
               
                 5 
                 4 
                 1 
                 4 
                 10 
                 5 
                 3 
                 3