Patent Publication Number: US-2021171611-A1

Title: Antibody that binds to envelope glycoprotein of sever fever with thrombocytopenia syndrome virus and use for same

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a divisional application of U.S. application Ser. No. 16/086,761, filed on Sep. 20, 2018, which is a national phase application of PCT Application No. PCT/KR2017/003156, filed on Mar. 23, 2017, which claims the benefit and priority to Korean patent application No. 10-2016-0034727, filed on Mar. 23, 2016. The entire disclosures of the applications identified in this paragraph are incorporated herein by references. 
    
    
     TECHNICAL FIELD 
     The present invention relates to an antibody which specifically binds to the envelope glycoprotein of severe fever with thrombocytopenia syndrome virus (SFTSV), the pathogen of severe fever with thrombocytopenia syndrome (SFTS), and is used in order to detect or diagnosis SFTSV and treat SFTS. 
     BACKGROUND 
     Severe Fever with Thrombocytopenia Syndrome (SFTS) is a new kind of mite-mediated infectious disease, and is mostly occurred by Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) mediated by  Haemaphysalis longicornis  or  Amblyomma testudinarium . SFTS was firstly reported in China in 2009, and the disease and virus was reported in Japan and Korea in 2012. The main symptoms of SFTS are fever, abdominal pain, nausea, vomiting, thrombocytopenia or leukopenia, etc., and in case of serious case, multiple organ failure may occur and result in death. SFTS has consistently occurred in China, Japan or Korea every year, and the fatality rate caused thereby is very high, and it mostly occurs in the period between spring and summer. A black-stripped field mouse is probable as the wild host of SFTSV, and it was presumed that domestic animals can play a role of host, since the serum antibody was found at the high ratio in domestic animals such as goat, cow, dog or chicken, etc. in the major outbreak areas of China. It has been reported that the infection from person to person occurred by mediating a body fluid of an infected person, but there is no approved therapeutic agent or prevention method to effectively treat SFTS until now. 
     There is a method of confirming an anti-SFTSV antibody titer in blood to confirm SFTS infection. Then the anti-SFTSV antibody titer is mostly measured with an antibody for N protein of SFTSV. The antibody is an antibody for SFTSV internal protein exposed when SFTSV becomes extinct. Thus, the conventional diagnosis by confirming the anti-SFTSV antibody titer has limitation that the existence of virus which is alive and actively acts cannot be accurately figured out. As another method of diagnosing SFTS, the method for detecting the RNA sequence of SFTSV in a subject derived from a human body has been known as having high accuracy, but it has a difficulty to isolate virus RNA of good quality from the subject. 
     On the other hand, International patent publication No. 2015/053455 (WO2015/053455A1) discloses the method for detecting an antibody for SFTSV, but specifically it does not disclose to which antigen of SFTSV the antibody binds and the neutralization activity of the antibody at all. 
     Thus, the development of an antibody or method which can effectively detect, isolate or purify SFTSV by recovering limitations of an inaccurate virus titer measurement method of conventional enzyme immunoreaction diagnosis method detecting the amount of killed SFTSV protein, or conventional low purity of virus RNA isolation method in blood is need. 
     DISCLOSURE 
     Technical Problem 
     The problem to be solved by the present invention is to provide an antibody which can effectively detect or diagnose SFTSV and treat SFTS. In addition, the other problem to be solved by the present invention is to provide an antibody which specifically binds to SFTSV, particularly an envelope glycoprotein of SFTSV. 
     Technical Solution 
     To solve the technical problems, the present invention provides a novel antibody which specifically binds to SFTSV, particularly its envelope glycoprotein. In addition, the present invention provides a method for effectively detecting, isolating or purifying SFTSV using the antibody. In addition, the present invention, a method for effectively preventing or treating SFTS using the antibody. 
     As the result that the present inventors have made extensive efforts to overcome the limitations of conventional diagnosis methods of SFTSV, they found a novel antibody which specifically binds to an envelope glycoprotein of SFTSV, particularly Gc or Gn, and found that SFTSV can be effectively detected using it, to complete the present invention. 
     SFTSV is a minus single strand RNA virus, and belongs to Bunyaviridae family, phlebovirus species. The virus is a globular virus of 80-100 nm diameter and uses  Haemaphysalis longicornis  as a mediator. The genome of the virus consists of large (L), Medium (M) and small (S) segments, and these encode 6 proteins of RNA dependent RNA polymerase (RdRp), glycoprotein precursor (M), glycoprotein N (Gn), glycoprotein C (Gc), nucleocapsid protein (NP) and non-structural protein (NSs). 
     In the present invention, an “antibody” may include whole antibodies and any antigen binding portion or single chains thereof. A naturally occurring “antibody” is a glycoprotein comprising at least two heavy chains (H) and two light chains (L) interconnected by disulfide bonds. Each heavy chain consists of a heavy chain variable region (VH) and a heavy chain constant region (CH). The heavy chain constant region consists of three domains, CH1, CH2 and CH3. Each light chain consists of a light chain variable region (VL) and a light chain constant region (CL). The light chain constant region consists of one domain, CL. The VH and VL regions may be further subdivided into regions of hypervariability, referred to as complementarity determining regions (CDR), interspersed with regions that are more conserved, referred to as framework regions (FR). Each VH and VL consists of three CDRs and four FRs arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen. 
     The present invention provides an antibody which specifically binds to an envelope glycoprotein of SFTSV, particularly an envelope glycoprotein of SFTSV, Gc or Gn. Preferably, the antibody may be comprise a specific amino acid sequence as follows or consists of them. In addition, certain modifications which are obvious in constant regions of heavy chains and light chains are included in the scope of the present invention in the range having same or similar binding specificity. Furthermore, as each of those antibodies can bind to the envelope glycoprotein of SFTSV, an antibody binding to other envelope glycoproteins of SFTSV of the present invention can be produced by mixing and matching VH, VL, full length light chain and full length heavy chain sequences (amino acid sequences and nucleotide sequences encoding the amino acid sequences). 
     In one example, the amino acid sequences of antibody clones (Ab1-5) which binds to Gc envelope glycoprotein of the present invention are shown in the following Tables 1-8. 
     Amino acid sequences of light chains and heavy chains binding to Gc envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 SEQ 
                   
                   
               
               
                 ID 
                 Antibody 
                   
               
               
                 NO 
                 and site 
                 Sequence 
               
               
                   
               
             
            
               
                  1 
                 light chain 
                 ELTLTQSPATLSLSPGETATLSCGASQSVSTNYLA 
               
               
                   
                 of Ab1 
                 WYQQKPGLAPRLLIY DASSRAT 
               
               
                   
                   
                 GIPDRFSGSGSGTDFTLTISRLAPEDSAVYYC QQYGSSPLT 
               
               
                   
                   
                 FGGGTKLEIK 
               
               
                   
               
               
                  2 
                 light chain 
                 ELVVTQPPSVSGAPGQRVTISC SGSSSNIGNNTVN 
               
               
                   
                 of Ab2 
                 WYQQLPGTAPKLLIY SNNQRPS 
               
               
                   
                   
                 GVPDRFSGSKSGTSASLAITGLQADDEADYYC QSFDSSLNDWV 
               
               
                   
                   
                 FGGGTKLTVL 
               
               
                   
               
               
                  3 
                 light chain 
                 ELELTQPPSVSGAPGQRVTISC TGSSSNIGAGYDVH 
               
               
                   
                 of Ab3 
                 WYQQLPGTAPKLLIY GNSNRPS 
               
               
                   
                   
                 GVPDRFSGSKSDTSASLAISGLRSEDEADYYC AAWDDSLNGQVV 
               
               
                   
                   
                 FGGGTKLTVL 
               
               
                   
               
               
                  4 
                 light chain 
                 ELVLTQPPSASGTPGQRVTISC SGSSSNIGSNTVN 
               
               
                   
                 of Ab4 
                 WYQQLPGTAPKLLIY SNNQRPP 
               
               
                   
                   
                 GVPDRFSGSKSGTSASLAISGLQSEDEADYYC QSYDSSLSYV 
               
               
                   
                   
                 FGTGTKVTVL 
               
               
                   
               
               
                  5 
                 light chain 
                 ELVVTQEPSLTVPPGGTVTLTC GSSTGPVTTTQYPY 
               
               
                   
                 of Ab5 
                 WFQQKPGQAPRTLIY DTNNRHP 
               
               
                   
                   
                 WTPARFSGSLLGGKAALTLSGAQPEDDA-YYC LLTSASAPWV 
               
               
                   
                   
                 FGGGTKLTVL 
               
               
                   
               
               
                  6 
                 heavy chain 
                 QVQLVQSGPEVKKPGSSVKVSCKAS GGTFSTYAIS 
               
               
                   
                 of Ab1 
                 WVRQAPGQGLEWMG GIIPISGTANYAQKFQG 
               
               
                   
                   
                 RVTITADESTSTAYMELSSLRSEDTAVYYCA VPV--------- 
               
               
                   
                   
                 VPAASGPFDYWG QGTLVTVSS 
               
               
                   
               
               
                  7 
                 heavy chain 
                 EVQLVESGGGLVKPGGSLRLSCAAS GFTFSSYSMN 
               
               
                   
                 of Ab2 
                 WVRQAPGKGLEWVS SISSSSRYIFYADSVKG 
               
               
                   
                   
                 RFTISRDNAKNSLYLQMNSLRAEDTAVYYCA 
               
               
                   
                   
                 SLGYCSGGSCYGFPEGGNAFDIWG QGTMVTVSS 
               
               
                   
               
               
                  8 
                 heavy chain 
                 QVQLQESGPGLVKPSETLSLTCTVS GGSFSGYYWS 
               
               
                   
                 of Ab3 
                 WIRQPPGKGLEWIG EIIHSGSTNYNPSLKS 
               
               
                   
                   
                 RVTISVDTSKNQFSLKLSSVTAADTAVYYCA RGDYYD--------- 
               
               
                   
                   
                 SSGAFDYWG QGTLVTVSS 
               
               
                   
               
               
                  9 
                 heavy chain 
                 EVQLVESGGGLVQPGGSLRLSCAAS GFTFSSYSMN 
               
               
                   
                 of Ab4 
                 WVRQAPGKGLEWVS SISSSSRYIFYADSVKG 
               
               
                   
                   
                 RFTISRDNAKNSLYLQMNSLRAEDTAVYY-- 
               
               
                   
                   
                 SLGYCSGGSCYGFPEGGNAFDIWG QGTMVTVSS 
               
               
                   
               
               
                 10 
                 heavy chain 
                 QVQLVQSGGGLVQPGGSLRLSCSAS GFTFSSYAMH 
               
               
                   
                 of Ab5 
                 WVRQAPGKGLEYVS AISSDGGSTYYADSVKG 
               
               
                   
                   
                 RFTISRDNSKNTLYLQMSSLRAEDTAVYYCV NDG------------ 
               
               
                   
                   
                 SSNHFDYWG QGTLVTVSS 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain framework region 1 (LFR1 or HFR1) of the antibody binding to Gc envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                 SEQ ID 
                 Antibody 
                   
               
               
                 NO 
                 and site 
                 Sequence 
               
               
                   
               
             
            
               
                 11 
                 LFR1 of Ab1 
                 ELTLTQSPATLSLSPGETATLSC 
               
               
                   
               
               
                 12 
                 LFR1 of Ab2 
                 ELVVTQPPSVSGAPGQRVTISC 
               
               
                   
               
               
                 13 
                 LFR1 of Ab3 
                 ELELTQPPSVSGAPGQRVTISC 
               
               
                   
               
               
                 14 
                 LFR1 of Ab4 
                 ELVLTQPPSASGTPGQRVTISC 
               
               
                   
               
               
                 15 
                 LFR1 of Ab5 
                 ELVVTQEPSLTVPPGGTVTLTC 
               
               
                   
               
               
                 16 
                 HFR1 of Ab1 
                 QVQLVQSGPEVKKPGSSVKVSCKAS 
               
               
                   
               
               
                 17 
                 HFR1 of Ab2 
                 EVQLVESGGGLVKPGGSLRLSCAAS 
               
               
                   
               
               
                 18 
                 HFR1 of Ab3 
                 QVQLQESGPGLVKPSETLSLTCTVS 
               
               
                   
               
               
                 19 
                 HFR1 of Ab4 
                 EVQLVESGGGLVQPGGSLRLSCAAS 
               
               
                   
               
               
                 20 
                 HFR1 of Ab5 
                 QVQLVQSGGGLVQPGGSLRLSCSAS 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain complementarity determining region 1 (LCDR1 or HCDR1) of the antibody binding to Gc envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 21 
                 LCDR1 of Ab1 
                 GASQSVSTNYLA 
               
               
                   
               
               
                 22 
                 LCDR1 of Ab2 
                 SGSSSNIGNNTVN 
               
               
                   
               
               
                 23 
                 LCDR1 of Ab3 
                 TGSSSNIGAGYDVH 
               
               
                   
               
               
                 24 
                 LCDR1 of Ab4 
                 SGSSSNIGSNTVN 
               
               
                   
               
               
                 25 
                 LCDR1 of Ab5 
                 GSSTGPVTTTQYPY 
               
               
                   
               
               
                 26 
                 HCDR1 of Ab1 
                 GGTFSTYAIS 
               
               
                   
               
               
                 27 
                 HCDR1 of Ab2 
                 GFTFSSYSMN 
               
               
                   
               
               
                 28 
                 HCDR1 of Ab3 
                 GGSFSGYYWS 
               
               
                   
               
               
                 29 
                 HCDR1 of Ab4 
                 GFTFSSYSMN 
               
               
                   
               
               
                 30 
                 HCDR1 of Ab5 
                 GFTFSSYAMH 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain framework region 2 (LFR2 or HFR2) of the antibody binding to Gc envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 4 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 31 
                 LFR2 of Ab1 
                 WYQQKPGLAPRLLIY 
               
               
                   
               
               
                 32 
                 LFR2 of Ab2 
                 WYQQLPGTAPKLLIY 
               
               
                   
               
               
                 33 
                 LFR2 of Ab3 
                 WYQQLPGTAPKLLIY 
               
               
                   
               
               
                 34 
                 LFR2 of Ab4 
                 WYQQLPGTAPKLLIY 
               
               
                   
               
               
                 35 
                 LFR2 of Ab5 
                 WFQQKPGQAPRTLIY 
               
               
                   
               
               
                 36 
                 HFR2 of Ab1 
                 WVRQAPGQGLEWMG 
               
               
                   
               
               
                 37 
                 HFR2 of Ab2 
                 WVRQAPGKGLEWVS 
               
               
                   
               
               
                 38 
                 HFR2 of Ab3 
                 WIRQPPGKGLEWIG 
               
               
                   
               
               
                 39 
                 HFR2 of Ab4 
                 WVRQAPGKGLEWVS 
               
               
                   
               
               
                 40 
                 HFR2 of Ab5 
                 WVRQAPGKGLEYVS 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain complementarity determining region 2 (LCDR2 or HCDR2) of the antibody binding to Gc envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 5 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 41 
                 LCDR2 of Ab1 
                 DASSRAT 
               
               
                   
               
               
                 42 
                 LCDR2 of Ab2 
                 SNNQRPS 
               
               
                   
               
               
                 43 
                 LCDR2 of Ab3 
                 GNSNRPS 
               
               
                   
               
               
                 44 
                 LCDR2 of Ab4 
                 SNNQRPP 
               
               
                   
               
               
                 45 
                 LCDR2 of Ab5 
                 DTNNRHP 
               
               
                   
               
               
                 46 
                 HCDR2 of Ab1 
                 GIIPISGTANYAQKFQG 
               
               
                   
               
               
                 47 
                 HCDR2 of Ab2 
                 SISSSSRYIFYADSVKG 
               
               
                   
               
               
                 48 
                 HCDR2 of Ab3 
                 EIIHSGSTNYNPSLKS 
               
               
                   
               
               
                 49 
                 HCDR2 of Ab4 
                 SISSSSRYIFYADSVKG 
               
               
                   
               
               
                 50 
                 HCDR2 of Ab5 
                 AISSDGGSTYYADSVKG 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain framework region 3 (LFR3 or HFR3) of the antibody binding to Gc envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 6 
               
               
                   
               
               
                 SEQ 
                   
                   
               
               
                 ID 
                 Antibody 
                   
               
               
                 NO 
                 and site 
                 Sequence 
               
               
                   
               
             
            
               
                 51 
                 LFR3 of Ab1 
                 GIPDRFSGSGSGTDFTLTISRLAPEDSAVYYC 
               
               
                   
               
               
                 52 
                 LFR3 of Ab2 
                 GVPDRFSGSKSGTSASLAITGLQADDEADYYC 
               
               
                   
               
               
                 53 
                 LFR3 of Ab3 
                 GVPDRFSGSKSDTSASLAISGLRSEDEADYYC 
               
               
                   
               
               
                 54 
                 LFR3 of Ab4 
                 GVPDRFSGSKSGTSASLAISGLQSEDEADYYC 
               
               
                   
               
               
                 55 
                 LFR3 of Ab5 
                 WTPARFSGSLLGGKAALTLSGAQPEDDA-YYC 
               
               
                   
               
               
                 56 
                 HFR3 of Ab1 
                 RVTITADESTSTAYMELSSLRSEDTAVYYCA 
               
               
                   
               
               
                 57 
                 HFR3 of Ab2 
                 RFTISRDNAKNSLYLQMNSLRAEDTAVYYCA 
               
               
                   
               
               
                 58 
                 HFR3 of Ab3 
                 RVTISVDTSKNQFSLKLSSVTAADTAVYYCA 
               
               
                   
               
               
                 59 
                 HFR3 of Ab4 
                 RFTISRDNAKNSLYLQMNSLRAEDTAVYY-- 
               
               
                   
               
               
                 60 
                 HFR3 of Ab5 
                 RFTISRDNSKNTLYLQMSSLRAEDTAVYYCV 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain complementarity determining region 3 (LCDR3 or HCDR3) of the antibody binding to Gc envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 7 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 61 
                 LCDR3 of Ab1 
                 QQYGSSPLT 
               
               
                   
               
               
                 62 
                 LCDR3 of Ab2 
                 QSFDSSLNDWV 
               
               
                   
               
               
                 63 
                 LCDR3 of Ab3 
                 AAWDDSLNGQVV 
               
               
                   
               
               
                 64 
                 LCDR3 of Ab4 
                 QSYDSSLSYV 
               
               
                   
               
               
                 65 
                 LCDR3 of Ab5 
                 LLTSASAPWV 
               
               
                   
               
               
                 66 
                 HCDR3 of Ab1 
                 VPV---------VPAASGPFDYWG 
               
               
                   
               
               
                 67 
                 HCDR3 of Ab2 
                 SLGYCSGGSCYGFPEGGNAFDIWG 
               
               
                   
               
               
                 68 
                 HCDR3 of Ab3 
                 RGDYYD---------SSGAFDYWG 
               
               
                   
               
               
                 69 
                 HCDR3 of Ab4 
                 SLGYCSGGSCYGFPEGGNAFDIWG 
               
               
                   
               
               
                 70 
                 HCDR3 of Ab5 
                 NDG------------SSNHFDYWG 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain framework region 4 (LFR4 or HFR4) of the antibody binding to Gc envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 8 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 71 
                 LFR4 of Ab1 
                 FGGGTKLEIK 
               
               
                   
               
               
                 72 
                 LFR4 of Ab2 
                 FGGGTKLTVL 
               
               
                   
               
               
                 73 
                 LFR4 of Ab3 
                 FGGGTKLTVL 
               
               
                   
               
               
                 74 
                 LFR4 of Ab4 
                 FGTGTKVTVL 
               
               
                   
               
               
                 75 
                 LFR4 of Ab5 
                 FGGGTKLTVL 
               
               
                   
               
               
                 76 
                 HFR4 of Ab1 
                 QGTLVTVSS 
               
               
                   
               
               
                 77 
                 HFR4 of Ab2 
                 QGTMVTVSS 
               
               
                   
               
               
                 78 
                 HFR4 of Ab3 
                 QGTLVTVSS 
               
               
                   
               
               
                 79 
                 HFR4 of Ab4 
                 QGTMVTVSS 
               
               
                   
               
               
                 80 
                 HFR4 of Ab5 
                 QGTLVTVSS 
               
               
                   
               
            
           
         
       
     
     In some exemplary embodiments, the antibody specifically binding to the envelope glycoprotein of SFTSV, Gc may comprise a light chain comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 1, 2, 3, 4 and 5, and a heavy chain comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 6, 7, 8, 9 and 10. The antibody consisting of these specific sequences can specifically and effectively bind to the envelope glycoprotein, Gc, and thus can be very usefully used for detection of SFTSV. 
     In another exemplary embodiment, preferably, the antibody which specifically binds to the envelope glycoprotein of SFTSV, Gc of the present invention can be provided as an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 1 and a heavy chain comprising an amino acid of SEQ ID NO 6, an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 2 and a heavy chain comprising an amino acid of SEQ ID NO 7, an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 3 and a heavy chain comprising an amino acid of SEQ ID NO 8, an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 4 and a heavy chain comprising an amino acid of SEQ ID NO 9, and an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 5 and a heavy chain comprising an amino acid of SEQ ID NO 10. 
     In another exemplary embodiment, the antibody which specifically binds to the envelope glycoprotein of SFTSV, Gc of the present invention can comprise a light chain complementarity determining region 1 (LCDR1) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 21, 22, 23, 24 and 25, a light chain complementarity determining region 2 (LCDR2) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 41, 42, 43, 44 and 45, a light chain complementarity determining region 3 (LCDR3) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 61, 62, 63, 64 and 65, a heavy chain complementarity determining region 1 (HCDR1) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 26, 27, 28, 29 and 30, a heavy chain complementarity determining region 2 (HCDR2) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 46, 47, 48, 49 and 50, and a heavy chain complementarity determining region 3 (HCDR3) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 66, 67, 68, 69 and 70. 
     In another exemplary embodiment, the antibody which specifically binds to the envelope glycoprotein of SFTSV, Gc of the present invention can be provided as an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 21, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 41, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 61, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 26, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 46, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 66; an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 22, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 42, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 62, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 27, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 47, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 67; an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 23, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 43, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 63, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 28, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 48, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 68; an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 24, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 44, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 64, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 29, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 49, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 69; or an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 25, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 45, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 65, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 30, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 50, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 70. 
     In one example, the amino acid sequences of antibody clones (Ab6-10) which binds to Gn envelope glycoprotein of the present invention are shown in the following Tables 9-16. 
     Amino acid sequences of light chains and heavy chains binding to Gn envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 9 
               
               
                   
               
               
                 SEQ 
                   
                   
               
               
                 ID 
                 Antibody 
                   
               
               
                 NO 
                 and site 
                 Sequence 
               
               
                   
               
             
            
               
                 81 
                 light chain 
                 ELALTQPPSVSVAPGKTAKITC GGDDIGSKTVQ 
               
               
                   
                 of Ab6 
                 WYQQTSGQAPVLVVY DDSDRPS 
               
               
                   
                   
                 GIPERFSGANSGNTATLTISRVEAGDEADYYC QVWDGRSDHVV 
               
               
                   
                   
                 FGGGTKLTVL 
               
               
                   
               
               
                 82 
                 light chain 
                 ELVLTQPPSVSAAPGQKVTISC SGSSSNIGNNVVS 
               
               
                   
                 of Ab7 
                 WYQQLPGTAPKLLIY DDNRRPS 
               
               
                   
                   
                 GIPDRFSGSKSGTSATLDITGLQTGDEADYYC 
               
               
                   
                   
                 ATWDGSLTAGRVL FGSGTKLTVL 
               
               
                   
               
               
                 83 
                 light chain 
                 ELALTQPPSVSVAPAMTAKITC GGDDIGSTTVQ 
               
               
                   
                 of Ab8 
                 WYQQTSGQAPVLVVY DDSDRPS 
               
               
                   
                   
                 GIPERFSGANSGNTATLTISRVEAGDEADYYC QVWDGRSDHVV 
               
               
                   
                   
                 FGGGTKLTVL 
               
               
                   
               
               
                 84 
                 light chain 
                 ELELTQPPSVSGTPGKRVSMSC SGSRSNIGGNVVN 
               
               
                   
                 of Ab9 
                 WYQQLPGKAPKLFIY NNDQRPS 
               
               
                   
                   
                 GVPDRVSGSKSGTSVSVAISGLQPEDEADYYC AAWDDILNGVV 
               
               
                   
                   
                 FGGGTQLTVL 
               
               
                   
               
               
                 85 
                 light chain 
                 ELVMTQSPSSLSASVGDTVTITC RASQSIYTYLN 
               
               
                   
                 of Ab10 
                 WYHQTPGKAPKLLIS AASSLQS 
               
               
                   
                   
                 GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQYADVPVT 
               
               
                   
                   
                 FGGGTKLEIK 
               
               
                   
               
               
                 86 
                 heavy chain 
                 QVQLVQSGAEVKKPGESLKISCKGS GYIFTNYWIG 
               
               
                   
                 of Ab6 
                 WVRQMPGKGLEWM GIIYPGDSDTRYSPSFQG 
               
               
                   
                   
                 QVTISADRSISTAYLQWSSLKASDTAMYYCA 
               
               
                   
                   
                 RLKLRGFSGGYGSGRRYFDYWG QGTLVTVSS 
               
               
                   
               
               
                 87 
                 heavy chain 
                 QVQLVQSGAEVKKPGESLKISCKGS GYSFTSYWIG 
               
               
                   
                 of Ab7 
                 WVRQMPGKGLEWM GIIYPGDSDTRYSPSFQG 
               
               
                   
                   
                 QVTISADKSISTAYLQWSSLKASDTAMYYCA 
               
               
                   
                   
                 RLKLRGFSGGYGSGSRYFDYWG QGTLVTVSS 
               
               
                   
               
               
                 88 
                 heavy chain 
                 QVQLVQSGAEVKKPGESLKISCKGS GYIFTNYWIG 
               
               
                   
                 of Ab8 
                 WVRQMPGKGLEWM GIIYPGDSDTRYSPSFQG 
               
               
                   
                   
                 QVTISADRSISTANLQWSSLKASDTALYYCA 
               
               
                   
                   
                 RLKLRGFSGGYGSGRRYFDYWG QGTLVTVSS 
               
               
                   
               
               
                 89 
                 heavy chain 
                 QVQLVQSGAEVKKPGESLKISCKGS GYNFTNYWIG 
               
               
                   
                 of Ab9 
                 WVRQLPGKGLEWM GIIYPGDSDTRYSPSFQG 
               
               
                   
                   
                 QVTISADKSISTAYLQWSSLKASDTAMYYCA RIRVIGFYD-- 
               
               
                   
                   
                 SSPPPLFDYWG QGTLVTVSS 
               
               
                   
               
               
                 90 
                 heavy chain 
                 EVQLVESGGGVVQPGRSLRLSCAAS GFTFSGYGIH 
               
               
                   
                 of Ab10 
                 WVRQAPGKGLEWV ALISYDGSNKYYADSVKG 
               
               
                   
                   
                 RFTISRDNSKNTLYLQMNSLRAEDTAVYYCA KDR-----DYFGSG-- 
               
               
                   
                   
                 FFDYWG QGTLVTVSS 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain framework region 1 (LFR1 or HFR1) of the antibody binding to Gn envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 10 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 91 
                 LFR1 of Ab6 
                 ELALTQPPSVSVAPGKTAKITC 
               
               
                   
               
               
                 92 
                 LFR1 of Ab7 
                 ELVLTQPPSVSAAPGQKVTISC 
               
               
                   
               
               
                 93 
                 LFR1 of Ab8 
                 ELALTQPPSVSVAPAMTAKITC 
               
               
                   
               
               
                 94 
                 LFR1 of Ab9 
                 ELELTQPPSVSGTPGKRVSMSC 
               
               
                   
               
               
                 95 
                 LFR1 of Ab10 
                 ELVMTQSPSSLSASVGDTVTITC 
               
               
                   
               
               
                 96 
                 HFR1 of Ab6 
                 QVQLVQSGAEVKKPGESLKISCKGS 
               
               
                   
               
               
                 97 
                 HFR1 of Ab7 
                 QVQLVQSGAEVKKPGESLKISCKGS 
               
               
                   
               
               
                 98 
                 HFR1 of Ab8 
                 QVQLVQSGAEVKKPGESLKISCKGS 
               
               
                   
               
               
                 99 
                 HFR1 of Ab9 
                 QVQLVQSGAEVKKPGESLKISCKGS 
               
               
                   
               
               
                 100 
                 HFR1 of Ab10 
                 EVQLVESGGGVVQPGRSLRLSCAAS 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain complementarity determining region 1 (LCDR1 or HCDR1) of the antibody binding to Gn envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 11 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 101 
                 LCDR1 of Ab6 
                 GGDDIGSKTVQ 
               
               
                   
               
               
                 102 
                 LCDR1 of Ab7 
                 SGSSSNIGNNVVS 
               
               
                   
               
               
                 103 
                 LCDR1 of Ab8 
                 GGDDIGSTTVQ 
               
               
                   
               
               
                 104 
                 LCDR1 of Ab9 
                 SGSRSNIGGNVVN 
               
               
                   
               
               
                 105 
                 LCDR1 of Ab10 
                 RASQSIYTYLN 
               
               
                   
               
               
                 106 
                 HCDR1 of Ab6 
                 GYIFTNYWIG 
               
               
                   
               
               
                 107 
                 HCDR1 of Ab7 
                 GYSFTSYWIG 
               
               
                   
               
               
                 108 
                 HCDR1 of Ab8 
                 GYIFTNYWIG 
               
               
                   
               
               
                 109 
                 HCDR1 of Ab9 
                 GYNFTNYWIG 
               
               
                   
               
               
                 110 
                 HCDR1 of Ab10 
                 GFTFSGYGIH 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain framework region 2 (LFR2 or HFR2) of the antibody binding to Gn envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 12 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 111 
                 LFR2 of Ab6 
                 WYQQTSGQAPVLVVY 
               
               
                   
               
               
                 112 
                 LFR2 of Ab7 
                 WYQQLPGTAPKLLIY 
               
               
                   
               
               
                 113 
                 LFR2 of Ab8 
                 WYQQTSGQAPVLVVY 
               
               
                   
               
               
                 114 
                 LFR2 of Ab9 
                 WYQQLPGKAPKLFIY 
               
               
                   
               
               
                 115 
                 LFR2 of Ab10 
                 WYHQTPGKAPKLLIS 
               
               
                   
               
               
                 116 
                 HFR2 of Ab6 
                 WVRQMPGKGLEWM 
               
               
                   
               
               
                 117 
                 HFR2 of Ab7 
                 WVRQMPGKGLEWM 
               
               
                   
               
               
                 118 
                 HFR2 of Ab8 
                 WVRQMPGKGLEWM 
               
               
                   
               
               
                 119 
                 HFR2 of Ab9 
                 WVRQLPGKGLEWM 
               
               
                   
               
               
                 120 
                 HFR2 of Ab10 
                 WVRQAPGKGLEWV 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain complementarity determining region 2 (LCDR2 or HCDR2) of the antibody binding to Gn envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 13 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 121 
                 LCDR2 of Ab6 
                 DDSDRPS 
               
               
                   
               
               
                 122 
                 LCDR2 of Ab7 
                 DDNRRPS 
               
               
                   
               
               
                 123 
                 LCDR2 of Ab8 
                 DDSDRPS 
               
               
                   
               
               
                 124 
                 LCDR2 of Ab9 
                 NNDQRPS 
               
               
                   
               
               
                 125 
                 LCDR2 of Ab10 
                 AASSLQS 
               
               
                   
               
               
                 126 
                 HCDR2 of Ab6 
                 GIIYPGDSDTRYSPSFQG 
               
               
                   
               
               
                 127 
                 HCDR2 of Ab7 
                 GIIYPGDSDTRYSPSFQG 
               
               
                   
               
               
                 128 
                 HCDR2 of Ab8 
                 GIIYPGDSDTRYSPSFQG 
               
               
                   
               
               
                 129 
                 HCDR2 of Ab9 
                 GIIYPGDSDTRYSPSFQG 
               
               
                   
               
               
                 130 
                 HCDR2 of Ab10 
                 ALISYDGSNKYYADSVKG 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain framework region 3 (LFR3 or HFR3) of the antibody binding to Gn envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 14 
               
               
                   
               
               
                 SEQ 
                   
                   
               
               
                 ID 
                 Antibody 
                   
               
               
                 NO 
                 and site 
                 Sequence 
               
               
                   
               
             
            
               
                 131 
                 LFR3 of Ab6 
                 GIPERFSGANSGNTATLTISRVEAGDEADYYC 
               
               
                   
               
               
                 132 
                 LFR3 of Ab7 
                 GIPDRFSGSKSGTSATLDITGLQTGDEADYYC 
               
               
                   
               
               
                 133 
                 LFR3 of Ab8 
                 GIPERFSGANSGNTATLTISRVEAGDEADYYC 
               
               
                   
               
               
                 134 
                 LFR3 of Ab9 
                 GVPDRVSGSKSGTSVSVAISGLQPEDEADYYC 
               
               
                   
               
               
                 135 
                 LFR3 of Ab10 
                 GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
               
               
                 136 
                 HFR3 of Ab6 
                 QVTISADRSISTAYLQWSSLKASDTAMYYCA 
               
               
                   
               
               
                 137 
                 HFR3 of Ab7 
                 QVTISADKSISTAYLQWSSLKASDTAMYYCA 
               
               
                   
               
               
                 138 
                 HFR3 of Ab8 
                 QVTISADRSISTANLQWSSLKASDTALYYCA 
               
               
                   
               
               
                 139 
                 HFR3 of Ab9 
                 QVTISADKSISTAYLQWSSLKASDTAMYYCA 
               
               
                   
               
               
                 140 
                 HFR3 of Ab10 
                 RFTISRDNSKNTLYLQMNSLRAEDTAVYYCA 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain complementarity determining region 3 (LCDR3 or HCDR3) of the antibody binding to Gn envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 15 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 141 
                 LCDR3 of Ab6 
                 QVWDGRSDHVV 
               
               
                   
               
               
                 142 
                 LCDR3 of Ab7 
                 ATWDGSLTAGRVL 
               
               
                   
               
               
                 143 
                 LCDR3 of Ab8 
                 QVWDGRSDHVV 
               
               
                   
               
               
                 144 
                 LCDR3 of Ab9 
                 AAWDDILNGVV 
               
               
                   
               
               
                 145 
                 LCDR3 of Ab10 
                 QQYADVPVT 
               
               
                   
               
               
                 146 
                 HCDR3 of Ab6 
                 RLKLRGFSGGYGSGRRYFDYWG 
               
               
                   
               
               
                 147 
                 HCDR3 of Ab7 
                 RLKLRGFSGGYGSGSRYFDYWG 
               
               
                   
               
               
                 148 
                 HCDR3 of Ab8 
                 RLKLRGFSGGYGSGRRYFDYWG 
               
               
                   
               
               
                 149 
                 HCDR3 of Ab9 
                 RIRVIGFYD--SSPPPLFDYWG 
               
               
                   
               
               
                 150 
                 HCDR3 of Ab10 
                 KDR-----DYFGSG--FFDYWG 
               
               
                   
               
            
           
         
       
     
     Amino acid sequences of light chain or heavy chain framework region 4 (LFR4 or HFR4) of the antibody binding to Gn envelope glycoprotein. 
     
       
         
           
               
               
               
             
               
                 TABLE 16 
               
               
                   
               
               
                 SEQ ID 
                 Antibody and 
                   
               
               
                 NO 
                 site 
                 Sequence 
               
               
                   
               
             
            
               
                 151 
                 LFR4 of Ab6 
                 FGGGTKLTVL 
               
               
                   
               
               
                 152 
                 LFR4 of Ab7 
                 FGSGTKLTVL 
               
               
                   
               
               
                 153 
                 LFR4 of Ab8 
                 FGGGTKLTVL 
               
               
                   
               
               
                 154 
                 LFR4 of Ab9 
                 FGGGTQLTVL 
               
               
                   
               
               
                 155 
                 LFR4 of Ab10 
                 FGGGTKLEIK 
               
               
                   
               
               
                 156 
                 HFR4 of Ab6 
                 QGTLVTVSS 
               
               
                   
               
               
                 157 
                 HFR4 of Ab7 
                 QGTLVTVSS 
               
               
                   
               
               
                 158 
                 HFR4 of Ab8 
                 QGTLVTVSS 
               
               
                   
               
               
                 159 
                 HFR4 of Ab9 
                 QGTLVTVSS 
               
               
                   
               
               
                 160 
                 HFR4 of Ab10 
                 QGTLVTVSS 
               
               
                   
               
            
           
         
       
     
     In one exemplary embodiment, the antibody which specifically binds to the envelope glycoprotein of SFTSV, Gn of the present invention may comprise a light chain comprising any one of amino acid sequences selected from the group consisting of SEQ ID NO 81, 82, 83, 84 and 85, and a heavy chain comprising any one of amino acid sequences selected from the group consisting of SEQ ID NO 86, 87, 88, 89 and 90. The antibody consisting of these specific sequences can specifically and effectively bind to the envelope glycoprotein, Gn, and thus can be very usefully used for detection of SFTSV. 
     In another exemplary embodiment, preferably, the antibody which specifically binds to the envelope glycoprotein of SFTSV, Gn of the present invention can be provided as an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 81 and a heavy chain comprising an amino acid of SEQ ID NO 86, an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 82 and a heavy chain comprising an amino acid of SEQ ID NO 87, an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 83 and a heavy chain comprising an amino acid of SEQ ID NO 88, an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 84 and a heavy chain comprising an amino acid of SEQ ID NO 89, and an antibody comprising a light chain comprising an amino acid sequence of SEQ ID NO 85 and a heavy chain comprising an amino acid of SEQ ID NO 90. 
     In another exemplary embodiment, the antibody which specifically binds to the envelope glycoprotein of SFTSV, Gn of the present invention can comprise a light chain complementarity determining region 1 (LCDR1) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 101, 102, 103, 104 and 105, a light chain complementarity determining region 2 (LCDR2) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 121, 122, 123, 124 and 125, a light chain complementarity determining region 3 (LCDR3) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 141, 142, 143, 144 and 145, a heavy chain complementarity determining region 1 (HCDR1) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 106, 107, 108, 109 and 110, a heavy chain complementarity determining region 2 (HCDR2) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 126, 127, 128, 129 and 130, and a heavy chain complementarity determining region 3 (HCDR3) comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 146, 147, 148, 149 and 150. 
     In another exemplary embodiment, the antibody which specifically binds to the envelope glycoprotein of SFTSV, Gn of the present invention can be provided as an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 101, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 121, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 141, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 106, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 126, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 146; an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 102, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 122, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 142, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 107, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 127, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 147; an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 103, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 123, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 143, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 108, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 128, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 148; an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 104, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 124, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 144, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 109, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 129, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 149; or an antibody comprising a light chain complementarity determining region 1 (LCDR1) of SEQ ID NO 105, a light chain complementarity determining region 2 (LCDR2) of SEQ ID NO 125, a light chain complementarity determining region 3 (LCDR3) of SEQ ID NO 145, a heavy chain complementarity determining region 1 (HCDR1) of SEQ ID NO 110, a heavy chain complementarity determining region 2 (HCDR2) of SEQ ID NO 130, and a heavy chain complementarity determining region 3 (HCDR3) of SEQ ID NO 150. 
     In one exemplary embodiment, the antibody of the present invention may include an antibody comprising an amino acid which is a homologue of an antibody comprising heavy chains and light chains described in the above Table 1 or Table 9. In addition, the antibody of the present invention may comprise a light chain variable region comprising the LCDR1, LCDR2 and LCDR3 sequences, and a heavy chain variable region comprising HCDR1, HCDR2 and HCDR3 sequences, and at least one of these CDR sequences may have the antibody disclosed herein or a specific amino acid sequence based on its conservative modification. In addition, the antibody of the present invention may be an antibody possessing functional properties of antibody binding to the envelope glycoprotein of SFTSV, Gc or Gn, and may be an antibody which binds to a same epitope as an antibody comprising heavy chains and light chains disclosed in Table 1 or Table 9. Furthermore, the antibody of the present invention may be prepared using an antibody having one or more kinds of light chains or antibody sequences suggested herein as a starting material for engineering the modified antibody, and comprise all the antibodies having partially modified properties from the starting antibody. 
     In the present invention, the antibody may comprise a modification to the framework region in the light chain or heavy chain in order to improve properties of the antibody. In addition, the antibody may have at least 1×10 7  M −1 , 1×10 8  M −1 , 1×10 9  M −1 , 1×10 10  M −1  or 1×10 11  M −1  of affinity constant (KA) for the envelope glycoprotein of SFTSV. 
     In addition, the antibody of the present invention may be a complete human antibody which specifically binds to the SFTSV envelope glycoprotein. This can have further reduced antigenicity when administered into a human subject, compared with chimera antibody, etc. The human antibody may comprise a heavy chain or light chain variable region, or a full length of heavy chain or light chain that are products of or one derived from a specific germline sequence, when it is collected from a system using a variable region or full length chain human germ line immunoglobulin gene. Moreover, the antibody of the present invention may be a De-immunized antibody having antigenicity. 
     In addition, in the present invention, the antigen may be a bispecific or a multispecific antibody. The antibody or its antigen-binding fragment of the present invention may be a bispecific molecule binding to two or more of different binding sites or target molecules. 
     In some exemplary embodiments, the antibody of the present invention may be a monoclonal antibody which specifically binds to the envelope glycoprotein of SFTSV. For example, the antibody of the present invention may be a human or humanized monoclonal antibody or chimera antibody which specifically binds to the envelope glycoprotein of SFTSV, and the antibody of the present invention may comprise a human heavy chain constant region and a human light chain constant region. In addition, the antibody of the present invention may be a single chain antibody, and the antibody of the present invention may be a Fab fragment, and may be a scFv (Single-chain variable fragment), and may be an IgG isotype. Preferably, the antibody of the present invention may be the scFv. 
     In the present invention, the monoclonal antibody may be produced by common monoclonal antibody methods, and the synthesized antibody genes can be expressed and purified by inserting them into a vector for antibody expression, preferably pcDNA, pCI, pCMV or pCEP4. In addition, viral or carcinogenic transformation of B lymphocytes may be used, and it may be prepared on the basis of the sequence of murine monoclonal antibody prepared using a murine system. For example, using a standard molecule biology technology, a DNA encoding heavy chain and light chain immunoglobulins is obtained from a murine hybridoma, and a non-murine immunoglobulin sequence can be contained with it. 
     In some exemplary embodiments, the present invention provides an antibody comprising a framework in which an amino acid is substituted with an antibody framework from each human VH or VL germline sequence, or its antigen binding fragment. 
     In another exemplary embodiment, the present invention provides a nucleic acid comprising a nucleotide sequence encoding a polypeptide comprising a light chain comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 1, 2, 3, 4 and 5, and a polypeptide comprising a heavy chain comprising any one of amino acid sequences selected from the group consisting of SEQ ID NOs 6, 7, 8, 9 and 10. In one embodiment, the nucleic acid may be any one of nucleic acid sequences selected from the group consisting of SEQ ID NOs 161, 162, 163, 164, 165, 166, 167, 168, 169 and 170, and this is shown in the following Table 17 (The bolded parts are light chain variable regions (VL), and the underlined parts are heavy chain variable regions (VH)). 
     
       
         
           
               
               
               
             
               
                 TABLE 17 
               
               
                   
               
               
                 SEQ ID 
                   
                   
               
               
                 NO 
                 Antibody 
                 Nucleic acid sequence 
               
               
                   
               
             
            
               
                 161 
                 Ab1 
                 
                   GAGCTCACACTCACGCAGTCTCCAGCCACCCTGTCTTTGTCTCCAG 
                 
               
               
                   
                 scFv 
                 
                   GGGAAACAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTGTTAGCA 
                 
               
               
                   
                   
                 
                   CCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCA 
                 
               
               
                   
                   
                 
                   GGCTCCTCATCTATGATGCATCCAGCAGGGCCACTGGCATCCCAG 
                 
               
               
                   
                   
                 
                   ACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCA 
                 
               
               
                   
                   
                 
                   TCAGCAGACTGGCGCCTGAAGATTCTGCGGTGTATTACTGTCAGC 
                 
               
               
                   
                   
                 
                   AATATGGTAGCTCACCTCTCACTTTCGGCGGAGGGACCAAGCTGG 
                 
               
               
                   
                   
                   AGATCAAA GGTGGTTCCTCTAGATCTTCCTCCTCTGGTGGCGGTGG 
               
               
                   
                   
                 CTCGGGCGGTGGTGGG CAGGTGCAGCTGGTGCAGTCTGGGCCTGA   
               
               
                   
                   
                 
                   GGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCT 
                 
               
               
                   
                   
                 
                   GGAGGCACCTTCAGCACCTATGCTATCAGCTGGGTGCGACAGGCC 
                 
               
               
                   
                   
                 
                   CCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTCTG 
                 
               
               
                   
                   
                 
                   GTACAGCAAACTACGCACAGAAATTCCAGGGCAGAGTCACCATTAC 
                 
               
               
                   
                   
                 
                   CGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCT 
                 
               
               
                   
                   
                 
                   GAGATCTGAGGACACGGCCGTGTATTACTGTGCGGTACCAGTAGTA 
                 
               
               
                   
                   
                 
                   CCAGCTGCCAGCGGCCCTTTTGACTACTGGGGCCAGGGAACCCTG 
                 
               
               
                   
                   
                 
                   GTCACCGTCTCCTCAGCC 
                 
               
               
                   
               
               
                 162 
                 Ab2 
                 
                   GAGCTCGTGGTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGG 
                 
               
               
                   
                 scFv 
                 
                   GCAGAGGGTCACCATCTCCTGTTCTGGAAGCAGCTCCAACATCGG 
                 
               
               
                   
                   
                 
                   AAATAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC 
                 
               
               
                   
                   
                 
                   CAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCT 
                 
               
               
                   
                   
                 
                   GACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCC 
                 
               
               
                   
                   
                 
                   ATCACTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCCAG 
                 
               
               
                   
                   
                 
                   TCCTTTGACAGCAGCCTGAATGATTGGGTGTTCGGCGGGGGCACC 
                 
               
               
                   
                   
                   AAGCTGACCGTCCTAGGC GGTGGTTCCTCTAGATCTTCCTCCTCTG 
               
               
                   
                   
                 GTGGCGGTGGCTCGGGCGGTGGTGGG GAGGTGCAGCTGGTGGAG   
               
               
                   
                   
                 
                   TCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCC 
                 
               
               
                   
                   
                 
                   TGTGCAGCCTCTGGATTCACCTTCAGTAGCTATAGCATGAACTGGGT 
                 
               
               
                   
                   
                 
                   CCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCATCCATTAG 
                 
               
               
                   
                   
                 
                   TAGTAGTAGTCGTTACATATTCTACGCAGACTCAGTGAAGGGCCGAT 
                 
               
               
                   
                   
                 
                   TCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATG 
                 
               
               
                   
                   
                 
                   AACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGC 
                 
               
               
                   
                   
                 
                   CTAGGATATTGTAGTGGTGGTAGCTGCTACGGGTTCCCGGAAGGTG 
                 
               
               
                   
                   
                 
                   GGAATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTC 
                 
               
               
                   
                   
                 
                   TTCA 
                 
               
               
                   
               
               
                 163 
                 Ab3 
                 
                   GAGCTCGAGCTGACTCAGCCACCCTCAGTGTCTGGGGCCCCAGG 
                 
               
               
                   
                 scFv 
                 
                   GCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGG 
                 
               
               
                   
                   
                 
                   GGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC 
                 
               
               
                   
                   
                 
                   CCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGT 
                 
               
               
                   
                   
                 
                   CCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCAGCCTCCCTG 
                 
               
               
                   
                   
                 
                   GCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGT 
                 
               
               
                   
                   
                 
                   GCAGCATGGGATGACAGCCTGAATGGCCAGGTGGTATTCGGCGG 
                 
               
               
                   
                   
                   AGGCACCAAGCTGACCGTCCTAGGC GGTGGTTCCTCTAGATCTTC 
               
               
                   
                   
                 CTCCTCTGGTGGCGGTGGCTCGGGCGGTGGTGGGCAGGTGCAGCT 
               
               
                   
                   
                 GCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTC 
               
               
                   
                   
                 CCTCACCTGCACTGTCTCTGGTGGGTCCTTCAGTGGTTACTACTGG 
               
               
                   
                   
                 AGCTGGATCCGCCAGCCCCCAGGAAAGGGGCTGGAGTGGATTGGG 
               
               
                   
                   
                 GAAATCATTCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGA 
               
               
                   
                   
                 GTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAATTCTCCCTG 
               
               
                   
                   
                 AAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTG 
               
               
                   
                   
                 CGAGAGGTGATTATTATGATAGTAGTGGTGCCTTTGACTACTGGGG 
               
               
                   
                   
                 CCAGGGAACCCTGGTCACCGTCTCCTCA 
               
               
                   
               
               
                 164 
                 Ab4 
                 
                   GAGCTCGTGCTGACTCAGCCACCTTCAGCGTCTGGGACCCCCGGG 
                 
               
               
                   
                 scFv 
                 
                   CAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGA 
                 
               
               
                   
                   
                 
                   AGTAATACTGTAAACTGGTACCAGCAGCTCCCCGGAACGGCCCCC 
                 
               
               
                   
                   
                 
                   AAACTCCTCATCTATAGTAATAATCAGCGGCCCCCAGGGGTCCCT 
                 
               
               
                   
                   
                 
                   GACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCC 
                 
               
               
                   
                   
                 
                   ATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGCCAG 
                 
               
               
                   
                   
                 
                   TCCTATGACAGCAGCCTGAGTTATGTCTTCGGAACTGGCACCAAG 
                 
               
               
                   
                   
                   GTGACCGTCCTAGGC GGTGGTTCCTCTAGATCTTCCTCCTCTGGTG 
               
               
                   
                   
                 GCGGTGGCTCGGGCGGTGGTGGG GAGGTGCAGCTGGTGGAGTCT   
               
               
                   
                   
                 
                   GGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGT 
                 
               
               
                   
                   
                 
                   GCAGCCTCTGGATTCACCTTCAGTAGCTATAGCATGAACTGGGTCC 
                 
               
               
                   
                   
                 
                   GCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCATCCATTAGTA 
                 
               
               
                   
                   
                 
                   GTAGTAGTCGTTACATATTCTACGCAGACTCAGTGAAGGGCCGATTC 
                 
               
               
                   
                   
                 
                   ACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAA 
                 
               
               
                   
                   
                 
                   CAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGCCTA 
                 
               
               
                   
                   
                 
                   GGATATTGTAGTGGTGGTAGCTGCTACGGGTTCCCGGAAGGTGGG 
                 
               
               
                   
                   
                 
                   AATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTC 
                 
               
               
                   
                   
                 
                   A 
                 
               
               
                   
               
               
                 165 
                 Ab5 
                 
                   GAGCTCGTGGTGACCCAGGAGCCCTCACTGACTGTGCCCCCAGGA 
                 
               
               
                   
                 scFv 
                 
                   GGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGACCTGTCACC 
                 
               
               
                   
                   
                 
                   ACTACTCAGTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAGGCC 
                 
               
               
                   
                   
                 
                   CCCAGGACACTCATTTATGATACCAACAACAGACACCCCTGGACA 
                 
               
               
                   
                   
                 
                   CCTGCCCGCTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTG 
                 
               
               
                   
                   
                 
                   ACCCTTTCGGGAGCGCAGCCTGAGGATGACGCTTAGTATTATTGCT 
                 
               
               
                   
                   
                 
                   TGCTCACCTCTGCTAGCGCTCCTTGGGTGTTCGGCGGAGGCACCA 
                 
               
               
                   
                   
                   AGCTGACCGTCCTAGGC GGTGGTTCCTCTAGATCTTCCTCCTCTGG 
               
               
                   
                   
                 TGGCGGTGGCTCGGGCGGTGGTGGGCAGGTGCAGCTGGTGCAGT 
               
               
                   
                   
                 CTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCT 
               
               
                   
                   
                 GTTCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTC 
               
               
                   
                   
                 CGCCAGGCTCCAGGGAAGGGACTGGAATATGTTTCAGCTATTAGTA 
               
               
                   
                   
                 GTGATGGGGGTAGCACATACTACGCAGACTCCGTGAAGGGCAGATT 
               
               
                   
                   
                 CACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTTCAAATGA 
               
               
                   
                   
                 GCAGTCTGAGAGCTGAGGACACGGCTGTATATTACTGTGTGAACGA 
               
               
                   
                   
                 TGGCAGCTCGAACCATTTTGACTACTGGGGCCAGGGAACCCTGGTC 
               
               
                   
                   
                 ACCGTCTCCTCA 
               
               
                   
               
               
                 166 
                 Ab6 
                 
                   GAGCTCGCCCTGACTCAGCCTCCCTCCGTGTCAGTGGCCCCAGGA 
                 
               
               
                   
                 scFv 
                 
                   AAGACGGCCAAGATTACCTGTGGGGGTGACGACATTGGAAGTAAA 
                 
               
               
                   
                   
                 
                   ACTGTGCAATGGTACCAACAGACCTCAGGCCAGGCCCCTGTGCTG 
                 
               
               
                   
                   
                 
                   GTCGTCTATGACGATAGCGACCGGCCCTCAGGGATCCCTGAGCGA 
                 
               
               
                   
                   
                 
                   TTCTCCGGCGCCAACTCTGGGAACACGGCCACCCTGACCATCAGC 
                 
               
               
                   
                   
                 
                   AGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTG 
                 
               
               
                   
                   
                 
                   GGACGGCAGAAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCT 
                 
               
               
                   
                   
                   GACCGTCCTAGGC GGTGGTTCCTCTAGATCTTCCTCCTCTGGTGGC 
               
               
                   
                   
                 GGTGGCTCGGGCGGTGGTGGG CAGGTGCAGCTGGTGCAGTCTGG   
               
               
                   
                   
                 
                   AGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTAAG 
                 
               
               
                   
                   
                 
                   GGTTCTGGATACATCTTTACCAACTACTGGATCGGCTGGGTGCGCC 
                 
               
               
                   
                   
                 
                   AGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTG 
                 
               
               
                   
                   
                 
                   GTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCAC 
                 
               
               
                   
                   
                 
                   CATCTCAGCCGACAGGTCCATCAGCACCGCCTACCTGCAGTGGAGC 
                 
               
               
                   
                   
                 
                   AGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACTAA 
                 
               
               
                   
                   
                 
                   AGCTCCGGGGGTTTTCGGGCGGCTATGGTTCAGGGAGACGCTACT 
                 
               
               
                   
                   
                 
                   TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 
                 
               
               
                   
               
               
                 167 
                 Ab7 
                 
                   GAGCTCGTGCTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGG 
                 
               
               
                   
                 scFv 
                 
                   ACTGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCTAACATTGG 
                 
               
               
                   
                   
                 
                   GAATAATGTTGTATCCTGGTACCAGCAACTCCCAGGAACAGCCCC 
                 
               
               
                   
                   
                 
                   CAAACTCCTCATTTATGACGATAACCGGCGACCCTCAGGGATTCCT 
                 
               
               
                   
                   
                 
                   GACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGAC 
                 
               
               
                   
                   
                 
                   ATCACCGGACTCCAGACTGGGGACGAGGCCGATTACTACTGCGCA 
                 
               
               
                   
                   
                 
                   ACATGGGATGGCAGCCTGACTGCTGGCCGTGTGTTGTTCGGCAGT 
                 
               
               
                   
                   
                   GGCACCAAGCTGACCGTCCTAGGT GGTGGTTCCTCTAGATCTTCCT 
               
               
                   
                   
                 CCTCTGGTGGCGGTGGCTCGGGCGGTGGTGGG CAGGTGCAGCTG   
               
               
                   
                   
                 
                   GTGCAGTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAG 
                 
               
               
                   
                   
                 
                   ATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGG 
                 
               
               
                   
                   
                 
                   CTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAT 
                 
               
               
                   
                   
                 
                   CATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAG 
                 
               
               
                   
                   
                 
                   GCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCT 
                 
               
               
                   
                   
                 
                   GCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGT 
                 
               
               
                   
                   
                 
                   GCGAGACTAAAGCTCCGGGGGTTTTCGGGCGGCTATGGTTCAGGG 
                 
               
               
                   
                   
                 
                   AGCCGCTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCT 
                 
               
               
                   
                   
                 
                   CCTCA 
                 
               
               
                   
               
               
                 168 
                 Ab8 
                 
                   GAGCTCGCCCTGACTCAGCCTCCCTCCGTGTCAGTGGCCCCAGCA 
                 
               
               
                   
                 scFv 
                 
                   ATGACGGCCAAGATTACCTGTGGGGGTGACGACATTGGAAGTACT 
                 
               
               
                   
                   
                 
                   ACTGTGCAATGGTACCAACAGACCTCAGGCCAGGCCCCTGTGCTG 
                 
               
               
                   
                   
                 
                   GTCGTCTATGACGATAGCGACCGGCCCTCAGGGATCCCTGAGCGA 
                 
               
               
                   
                   
                 
                   TTCTCCGGCGCCAACTCTGGGAACACGGCCACCCTGACCATCAGC 
                 
               
               
                   
                   
                 
                   AGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTG 
                 
               
               
                   
                   
                 
                   GGACGGCAGAAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCT 
                 
               
               
                   
                   
                   GACCGTCCTAGGC GGTGGTTCCTCTAGATCTTCCTCCTCTGGTGGC 
               
               
                   
                   
                 GGTGGCTCGGGCGGTGGTGGGCAGGTGCAGCTGGTGCAGTCTGG 
               
               
                   
                   
                 AGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCTGTAAG 
               
               
                   
                   
                 GGTTCTGGATACATCTTTACCAACTACTGGATCGGCTGGGTGCGCC 
               
               
                   
                   
                 AGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTG 
               
               
                   
                   
                 GTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCAC 
               
               
                   
                   
                 CATCTCAGCCGACAGGTCCATCAGCACCGCCAACCTGCAGTGGAG 
               
               
                   
                   
                 CAGCCTGAAGGCCTCGGACACCGCCCTGTATTACTGTGCGAGACTA 
               
               
                   
                   
                 AAGCTCCGGGGGTTTTCGGGCGGCTATGGTTCAGGGAGACGCTAC 
               
               
                   
                   
                 TTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 
               
               
                   
               
               
                 169 
                 Ab9 
                 
                   GAGCTCGAGCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGG 
                 
               
               
                   
                 scFv 
                 
                   AAGAGGGTCAGTATGTCTTGTTCTGGAAGTAGGTCCAACATCGGA 
                 
               
               
                   
                   
                 
                   GGTAATGTTGTGAACTGGTACCAGCAGCTCCCAGGAAAGGCCCCC 
                 
               
               
                   
                   
                 
                   AAACTCTTCATCTACAATAATGATCAGCGGCCCTCAGGGGTCCCTG 
                 
               
               
                   
                   
                 
                   ACCGAGTCTCTGGCTCCAAGTCAGGCACCTCAGTCTCCGTGGCCA 
                 
               
               
                   
                   
                 
                   TCAGTGGGCTCCAGCCTGAAGATGAGGCTGATTATTACTGTGCAG 
                 
               
               
                   
                   
                 
                   CTTGGGATGACATCCTGAATGGTGTGGTCTTCGGCGGAGGGACCC 
                 
               
               
                   
                   
                   AGCTGACCGTCCTCGGC GGTGGTTCCTCTAGATCTTCCTCCTCTGG 
               
               
                   
                   
                 TGGCGGTGGCTCGGGCGGTGGTGGG CAGGTGCAGCTGGTGCAGT   
               
               
                   
                   
                 
                   CTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCCT 
                 
               
               
                   
                   
                 
                   GTAAGGGTTCTGGATACAACTTCACCAACTACTGGATCGGGTGGGT 
                 
               
               
                   
                   
                 
                   GCGCCAGCTGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTA 
                 
               
               
                   
                   
                 
                   TCCTGGTGACTCCGACACCAGATATAGCCCGTCCTTCCAAGGCCAG 
                 
               
               
                   
                   
                 
                   GTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGT 
                 
               
               
                   
                   
                 
                   GGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGA 
                 
               
               
                   
                   
                 
                   GAATTCGAGTTATCGGATTCTATGATAGTAGCCCCCCGCCCTTATTT 
                 
               
               
                   
                   
                 
                   GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 
                 
               
               
                   
               
               
                 170 
                 Ab10 
                 
                   GAGCTCGTGATGACTCAGTCTCCATCTTCCCTGTCCGCATCTGTGG 
                 
               
               
                   
                 scFv 
                 
                   GAGACACAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTTACA 
                 
               
               
                   
                   
                 
                   CCTATTTAAATTGGTATCACCAGACACCAGGGAAAGCCCCTAAACT 
                 
               
               
                   
                   
                 
                   CCTGATTTCTGCTGCATCTAGTTTGCAAAGTGGTGTCCCATCAAGG 
                 
               
               
                   
                   
                 
                   TTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCAGC 
                 
               
               
                   
                   
                 
                   AGTCTGCAACCTGAGGATTTTGCAACGTACTACTGTCAACAGTATG 
                 
               
               
                   
                   
                 
                   CGGATGTCCCGGTCACTTTCGGCGGAGGGACCAAGCTGGAGATCA 
                 
               
               
                   
                   
                   AA GGTGGTTCCTCTAGATCTTCCTCCTCTGGTGGCGGTGGCTCGGG 
               
               
                   
                   
                 CGGTGGTGGGGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGG 
               
               
                   
                   
                 TCCAGCCTGG GAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATT   
               
               
                   
                   
                 
                   CACCTTCAGTGGCTATGGCATACACTGGGTCCGCCAGGCTCCAGGC 
                 
               
               
                   
                   
                 
                   AAGGGGCTGGAGTGGGTGGCACTTATATCATATGATGGAAGTAATA 
                 
               
               
                   
                   
                 
                   AATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA 
                 
               
               
                   
                   
                 
                   CAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCT 
                 
               
               
                   
                   
                 
                   GAGGACACGGCTGTGTATTACTGTGCGAAAGATCGGGATTACTTTG 
                 
               
               
                   
                   
                 
                   GTTCAGGGTTCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT 
                 
               
               
                   
                   
                 
                   CTCCTCA 
                 
               
               
                   
               
            
           
         
       
     
     In another exemplary embodiment, the antibody of the present invention may comprise an amino acid sequence having at least 90%, 95%, 97%, 98% or 99% sequence identity with any one of amino acid sequences disclosed in the above Tables 1-16, within the range that the antibody specificity to the envelope glycoprotein of SFTSV is maintained. In addition, a nucleic acid which can express the antibody of the present invention may comprise a nucleic acid having at least 90%, 95%, 97%, 98% or 99% sequence identity with any one of nucleic acid sequences disclosed in the above Table 17. 
     In addition, the present invention provides a vector and a host cell comprising the nucleic acid. The vector of the present invention may comprise a nucleic acid encoding an amino acid sequence of the antibody binding to the envelope glycoprotein of SFTSV, Gc, or a nucleic acid encoding an amino acid of the antibody binding to Gn. Otherwise, the vector of the present invention may express a bispecific antibody, by comprising all the two kinds of nucleic acids. 
     In one exemplary embodiment, the present invention provides (1) a first recombinant DNA fragment encoding a heavy chain of the antibody of the present invention, and (2) a second recombinant DNA fragment encoding a light chain of the antibody of the present invention. In another exemplary embodiment, the present invention provides a host cell comprising a recombinant DNA fragment encoding a heavy chain and a light chain of the present invention, respectively. In some exemplary embodiments, the antibody or its antigen binding fragment is a human monoclonal antibody or its antigen binding fragment. 
     To express a polynucleotide encoding the antibody binding to the envelope glycoprotein of SFTSV of the present invention, various expression vectors can be used. To produce an antibody in a mammalian host cell, both of virus-based or non-viral expression vector may be used. For example, vectors such as pcDNA, pCI, pCMV or pCEP4, and the like and host cells such as HEK293, CHO or CHO-DG44, and the like may be used. 
     The host cell possessing and expressing the antibody of the present invention may be a prokaryotic or eukaryotic cell. For example, the host cell may be  E. coli , preferably,  E. coli  ER2738. HB2151, BL21 and the like, and they may be useful for cloning and expressing the polynucleotide of the present invention. In addition, as other microbial hosts,  Bacillus , for example,  Bacillus subtilis  or other intestinal bacteria, for example,  Salmonella  or  Serratia , or various  Pseudomonas  species may be used. To express the antibody of the present invention, other microorganisms, for example, yeasts can be used, and an insect cell combined with a baculovirus vector may be also used. 
     In some preferable exemplary embodiments, a mammalian host cell may be used for expressing and preparing the SFTSV envelope glycoprotein binding polypeptide of the present invention. For example, it may be a hybridoma cell line expressing an endogenous immunoglobulin gene or a mammalian cell line possessing an exogenous expression vector. Further, it may comprise for example, CHO cell line, Cos cell line, HeLa cell, myeloma cell line, HEK cell line, transformed B-cell and hybridoma, as any animal or human cell. In addition, numerous appropriate host cell lines which can secret an immunoglobulin can be used, and preferably, HEK293, CHO or CHO-DG44 may be used. 
     In addition, the present invention provides a composition for diagnosing SFTSV comprising one or more kinds of SFTSV envelope glycoprotein binding molecules (for example, Gc or Gn binding antibody or its antigen binding fragment). The composition for diagnosis of the present invention may be usefully used for detection, isolation or purification of SFTSV. Moreover, the composition may further comprise one or more kinds of other agents appropriate for diagnosing SFTSV. In addition, the present invention provides a method for diagnosing SFTSV using the antibody of the present invention. The method may be used for quantitative or qualitative detection or diagnosis of SFTSV. Specifically, the diagnosis method may comprise a diagnosis examination to determine the expression of envelope glycoprotein and/or nucleic acid of SFTSV and the function of envelope glycoprotein of SFTSV from a biological sample (for example, blood, serum, cell or tissue) or a subject who is suffering from or at risk of developing SFTS. In the present invention, the detection includes quantitative and/or qualitative analysis, and includes detection of existence and absence and detection of virus titer, and this method has been known in the art, and those skilled in the art may select a proper method to conduct the present invention. 
     In the present invention, the detection of diagnosis or diagnosis of SFTSV may be detected by radio immunoassay, western blot, ELISA (Enzyme linked immunosorbent assay) or immune fluorescence assay, etc. which detects an antigen-antibody complex. In the present invention, an antigen may be labeled with a label such as a radioactive material, enzyme or fluorescent material, etc. 
     In one embodiment, the method of diagnosis of the present invention may use a complex in which the antibody to the envelope glycoprotein of SFTSV is conjugated to magnetic beads. Specifically, the method can more effectively detect, isolate or purify SFTSV, using the complex in which the antibody specific to the envelope glycoprotein of SFTSV, Gc or Gn is combined to magnetic beads. The antibody to the SFTSV envelope glycoprotein-magnetic bead complex combines with SFTSV existed in a subject using properties of the antibody and at that time, when the magnetic beads are pulled by magnetic power, viruses and other materials in the subject are separated, thereby effectively purifying the virus. The virus purified in this way is relatively useful for RNA isolation, as impurities are removed, and through this, purification result data of good quality can be obtained. In addition, an immunochemical response using another antibody can be processed for the virus attached to magnetic beads, and through this, SFTSV existed in the subject can be rapidly confirmed. The schematic figure of the diagnosis method was shown in  FIG. 4 . 
     In addition, the present invention provides a kit for diagnosing SFTSV comprising an antibody binding to an envelope glycoprotein of SFTSV. The kit may comprise any one or more aforementioned antibodies and a reagent for detecting an antigen-antibody complex. As the reagent for detecting an antigen-antibody complex, reagents used for radio immunoassay, ELISA (Enzyme linked immunosorbent assay) or immune fluorescence assay and the like may be used. 
     For example, for the detection of the immunoreaction, the detection reagent may be labeled directly or indirectly in the form of sandwich. In case of direct labeling method, a serum sample used for array, etc. may be labeled by a fluorescence label such as Cy3 or Cy5. In case of sandwich method, the detection may be performed by combining a target protein with a labeled detection antibody, after combining a non-labeled serum sample with an array in which a detection reagent is attached in advance. In case of sandwich method, as the sensitivity and specificity can be increased, the detection in the level of pg/mL is possible. Besides that, a radioactive material, a color material, a magnetic particle or a dense electron particle and the like may be used as a labeling material. A confocal microscope may be used for the fluorescence strength, and for example, may be obtained from Affymetrix, Inc. or Agilent Technologies, Inc, etc. 
     The kit of the present invention may further comprise one or more additional components needed for binding analysis, and for example, may further comprise a binding buffer, a reagent needed for sample preparation, a syringe for blood collection or negative and/or positive control. The kit of the present invention which can comprise various detection reagents may be provided for ELISA analysis, dip stick rapid kit analysis, microarray, gene amplification, or immunoassay, etc. according to analysis aspects, and proper detection reagents may be sorted according to the analysis aspects. 
     In addition, the present invention provides a pharmaceutical composition comprising the antibody binding to SFTSV envelope glycoprotein of the present invention. Preferably, the pharmaceutical composition may be used for prevention or treatment of SFTS. The antibody of the present invention can effectively prevent or treat SFTS, by neutralizing SFTSV and blocking proliferation of virus. 
     In the present invention, the composition may further contain one or more kinds of other agents appropriate for treating or preventing an SFTSV related disease. The carrier which can be used for the pharmaceutical composition may enhance the effect of composition, or stabilize the composition, or make preparation of the composition easy. The pharmaceutically acceptable carrier may comprise a physiologically acceptable solvent, a dispersive medium, a coating agent, an anti-bacterial agent, an anti-fungal agent, an isotonic agent or an absorption delaying agent and the like. 
     In the present invention, the pharmaceutical composition may be administered by a variety of methods known in the art, and the administration route and/or method may vary depending on the desired result. The pharmaceutical composition may be administered by administration methods, for example, intravenous, intramuscular, intraperitoneal or subcutaneous, and the like. According to the administration route, the active compound, antibody may be coated with a material protecting the compound from the action of acids and other natural conditions which may inactivate the compound. 
     In the present invention, the composition may be a sterile fluid. To maintain a proper fluidity, for example, a coating material such as lecithin or a surfactant may be used. In addition, the composition may comprise an isotonic agent (for example, sugar, polyalcohol, mannitol, sorbitol, and sodium chloride, etc.) or an absorption delaying agent (aluminum monostearate or gelatin, etc.). 
     In the present invention, the pharmaceutical composition may be prepared according to methods known in the art and commonly conducted, and preferably, may be prepared under GMP condition. The pharmaceutical composition may comprise a therapeutically effective dose or efficacious dose of the SFTSV envelope glycoprotein binding antibody. In addition, the dosage level of active ingredients in the pharmaceutical composition may be enough to achieve a therapeutic effect without toxicity to a patient. 
     In the present invention, the treatment dosage may be titrated to optimize safety and efficacy. When the antibody of the present invention is administered systemically, the range of dosage may be about 0.0001 to 100 mg, more commonly 0.01 to 15 mg per 1 kg of the host body weight. An exemplary treatment method entails systemic administration once per two weeks, or once per one month, or once per three months to 6 months. In some methods of systemic administration, the dosage is, and in some methods, the dosage may be adjusted to achieve the serum antibody concentration of 1 to 1000 μg/mL in some methods of systemic administration and 25 to 500 μg/mL in some methods. Otherwise, when less frequent administration is required, the antibody may be administered by a time-release agent. The dosage and frequency may be differed according to the half-life of the antibody in a patient. In prophylactic purposes, the relatively low dosage may be administered at relatively infrequent intervals for a long period of time. 
     In addition, the present invention provides a method for preventing or treating SFTS using the pharmaceutical composition. The prevention or treatment method may comprise administering the composition comprising the antibody of the present invention in an therapeutically effective amount. The “therapeutically effective amount” indicates an amount of the antibody of the present invention or the composition comprising thereof which is effective for prevention or treatment of SFTS diseases. 
     In addition, the present invention provides a use of an SFTSV envelope glycoprotein binding antibody for preparation of a composition for diagnosis of SFTSV. For the preparation of the composition for diagnosis, the antibody or composition comprising thereof of the present invention may comprise additional components such as an acceptable carrier, etc. 
     Furthermore, the present invention provides a use of an SFTSV envelope glycoprotein binding antibody. The antibody which specifically binds to SFTSV of the present invention may be used for SFTSV diagnosis, and may be used as a diagnosis use determining expression of the envelope glycoprotein and/or nucleic acid of SFTSV and the function of the protein from a subject who is suffering from or at risk of developing SFTS. In addition, the antibody of the present invention may be used as a use of prevention or treatment of SFTS occurred by SFTSV for a who is at risk of developing or suffering from SFTS. 
     Advantageous Effects 
     The antibody of the present invention can specifically bind to envelope glycoprotein of SFTSV, Gc or Gn, and thus SFTSV can be effectively detected or diagnosed and SFTS can be treated, using the antibody of the present invention. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG. 1  shows the amino acid sequences of antibody clones Ab1 to Ab10. 
         FIG. 2  shows the ELISA analysis result of scFv fragment antibody purified for SFTSV envelope glycoprotein Gc and Gn. These data show mean±S.D of 3 times repeated samples. 
         FIGS. 3A and 3B  is (A) the immune fluorescence analysis result and (B) the fluorescence strength measurement of SFTSV infection. In the immune fluorescence analysis result, it was shown that Vero cells infected by SFTSV reacted with the antibody to Gn, and it was shown that Ab10 inhibited the virus infection dose-dependently. Ab10 was significantly excellent in inhibiting virus invasion compared with MAb 4-5. 
         FIG. 4  is a schematic figure showing the method for detecting SFTSV using an antibody-magnetic bead complex. 
     
    
    
     DETAILED DESCRIPTION 
     Hereinafter, examples, etc. will be described in detail to facilitate understanding of the present invention. However, the examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The examples of the present invention are provided to describe the present invention more completely to those skilled in the art. 
     Example 1: Preparation of Cells 
     Vero cells derived from African green monkey kidneys were purchased from Korean Cell Line Bank, and cultured at 37° C. under 5% carbon dioxide circumstance with Roswell Park Memorial Institute (RPMI)-1640 medium (Welgene) supplemented with 2% heat inactivated fetal bovine serum (Gibco) and penicillin-streptomycin (Gibco). 
     Example 2: Preparation of Virus Strains 
     The SFTS virus used in the present experiment was KF358691 which was isolated from a serum sample of 63-year-old female patient who was hospitalized in Seoul National University hospital and dead in 2012 [Kim K H, Yi J, Kim G, Choi S J, Jun K I, Kim N H, et al. Severe fever with thrombocytopenia syndrome, South Korea, 2012.  Emerging infectious diseases.  2013; 19(11):1892-4]. The isolated virus was inoculated into a single layer of Vero cells and cultured at 37° C. under 5% carbon dioxide circumstance. The virus was proliferated in Vero cells and all the experiments were performed at the third viral passage of virus culturing. Using Reed-Muench method, 50% tissue culture infection dose (TCID50) was titrated in Vero cells. 
     Example 3: Preparation of Recombinant SFTS Virus Glycoprotein and Single Chain Variable Fragment Antibody Fusion Protein 
     The amino acid sequence of SFTS virus glycoprotein used in the present experiment was previously reported [Kim K H, Yi J, Kim G, Choi S J, Jun K I, Kim N H, et al. Severe fever with thrombocytopenia syndrome, South Korea, 2012. Emerging infectious diseases. 2013; 19(11):1892-4]. To get a DNA strand encoding the SFTS virus glycoprotein, a human codon optimized DNA sequence corresponding to the amino acid sequence of SFTS virus glycoprotein of SEQ ID NO 171 (GenBank Accession No: AGT98506, amino acids 20-452 for Gn glycoprotein, amino acids 563-1035 for Gc glycoprotein) was synthesized (GenScript). 
     To overexpress recombinant SFTS virus glycoprotein Gc and Gn which were fused to human immunoglobulin G1 (IgG1) Fc region (Gc-Fc, Gn-Fc) or fused to human Ig k-chain constant region (Gc-Ck, Gn-Ck), the SFTS glycoprotein-encoding gene was prepared according to the method disclosed in [Park S, Lee D H, Park J G, Lee Y T, Chung J. A sensitive enzyme immunoassay for measuring cotinine in passive smokers.  Clinica chimica acta; international journal of clinical chemistry  2010; 411(17-18): 1238-42], [Lee Y, Kim H, Chung J. An antibody reactive to the Gly63-Lys68 epitope of NT-proBNP exhibits O-glycosylation-independent binding.  Experimental  &amp;  molecular medicine.  2014; 46:e114]. 
     First of all, a DNA sequence obtained by amplifying the Fc region of human IgG1 using 2 kinds of primers (5′-GAGCCCAAATCTTGTGACAAAACTCAC-3′) and (5′-GGATCCTCATTTACCCGGGGACAGGGAG-3′) from human marrow-derived cDNA library (Clontech Laboratories), or the synthesized constant region of human Ig k-chain (UniProtKB/Swiss-Prot: P01834.1) was modified to be positioned at the DNA 3′ side of gene sequence to be added. The gene sequence to be added was cloned in a modified pCEP4 vector (Invitrogen) to enable gene addition by Sfil restriction enzyme. 
     The antibody clone was produced in the form of single chain variable fragment-human IgG1 Fc region fusion protein (scFv-Fc) using scFv coding DNA of each clone. Then, the vector was transfected into HEK293F cell (Invitrogen) using polyethyleneimine (Polysciences), and the transfected cell was cultured in FreeStyle™ 293 expression medium containing 100 U/L penicillin-streptomycin. The overexpressed recombinant SFTS virus glycoprotein fusion protein was purified through an affinity chromatography using A/KappaSelect column and AKTA pure chromatography system (GE Healthcare). 
     Example 4: Antibody Library Construction and Biopanning 
     Peripheral blood monocytes of patient recovered from SFTS were collected using Ficoll-Paque solution (GE Healthcare). The total RNAs were separated using TRIzol reagent (Invitrogen), and cDNA was synthesized from the total RNAs using SuperScript III first strand cDNA synthesis kit with oligo(dT) priming. Using the cDNA, the phage-display library of human single chain variable fragment (scFv) was constructed using pComb3XSS phagemid vector. In addition, to select scFv clone from the library, as disclosed in [Barbas C F, Burton D R, Scott J K, Silverman G J. Phage display: a laboratory manual: CSHL Press; 2004.], 4 rounds of biopanning were performed. 3 μg of recombinant SFTS virus glycoprotein Gc or Gn human IgG1 Fc region fusion protein (Gc-Fc, Gn-Fc) was used for coating 5×106 of magnetic Dynabeads M-270 epoxy beads (Invitrogen) according to the manufacturer&#39;s instruction for each round of biopanning. And then the beads bound with proteins were used for biopanning procedures. 
     Example 5: Screening of Single Chain Variable Fragment Antibody to SFTS Virus 
     To select an individual antibody clone which bound to SFTS virus glycoproteins, the phage clone was selected form the last round of biopanning, and scFv-display phage was prepared for phage enzyme immunoassay. Microtiter plate (Corning) was coated with 100 ng of recombinant Gc, Gn human Ig k-chain constant region fusion proteins (Gc-Ck, Gn-Ck) per well at 4° C. overnight. The well was blocked with 3% (w/v) BSA in 100 μl of PBS at 37° C. for 1 hour, and cultured with 50 μl of culture supernatant containing phage at 37° C. for 2 hours, and washed with 0.05% (v/v) Tween20 in 150 μl of PBS three times. Then, 50 ml of horseradish peroxidase (HRP)-bound anti-M13 antibody distilled in a blocking buffer (1:5000) was added to each well, and then the plate was cultured at 37° C. for 1 hour. After washing with 150 μl of 0.05% PBST, 50 μl of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) substrate solution (Pierce) was added to each well, and cultured at the room temperature for 30 minutes. And then the absorbance of each well was measured at 405 nm using a microplate reader (Labsystems). 
     Example 6: Neutralization Analysis 
     The SFTS virus specific scFv-Fc fusion antibody (100 μl/ml) was serially diluted to be decreased 10 folds each by 0.01 μl/ml. scFvs of each concentration was mixed in an equivalent volume of 100 TCID50 SFTS virus (strain KF358691) and cultured at 37° C. for 1 hours. Then, the virus-antibody mixture was transferred to the single layer of Vero cells in an 8-well confocal microscope chamber and cultured at 37° C. for 1 hour. After removing the virus-antibody mixture, samples were cultured in RPMI-1640 medium containing 2% FBS and antibiotics at 37° C. under 5% carbon dioxide circumstance. Vero cells in the 8-well confocal microscope chamber were used for immune fluorescence assay (IFA). All the experiments were performed three times and the relative neutralization effect was measured by comparing with MAb 4-5 [Xiling Guo et al. A human antibody neutralizing SFTS virus, an emerging hemorrhagic fever virus, 2013. Clin. Vaccine Immunol. 2013; 20(9):1426-32).] as a positive control and anti-newcastle disease virus (NDV) antibody as a negative control 
     Example 7: Immune Fluorescence Analysis (IFA) and Fluorescence Intensity Measurement 
     The relative neutralization effect was measured using immune fluorescence assay (IFA). Cells with or without treatment with virus-antibody mixture having or not having Ab10, MAb 4-5 (positive control), anti-NDV (negative control) were cultured for 2 days. The cells were fixed with 4% paraformaldehyde in phosphate-buffer saline (PBS) for 1 hour. After blocking and penetrating slides with 0.1% triton X-100 in 1% fetal bovine serum (BSA), they were cultured together with anti-SFTS virus glycoprotein Gn clone Ab6 antibody (5 μl/ml) at 4° C. overnight. The cells were washed and cultured with fluorescein isothiocyanate (FITC)-bound anti-human IgG (Pierce) at the room temperature for 1 hour. 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) was used for dying a nucleus. Samples were experimented with a confocal microscope (Leica, Buffalo Grove, Ill., USA). Fluorescence signal strength was measured using computer assisted Leica application suite advanced fluorescence (LAS AF). The microscope photographs were taken in 5 regions of each slide using ×10/0.3 lens, and 3 median values were used for analysis. DAPI signal was set with 405 nm blue diode laser and Alexa 488 was adjusted with an argon ion laser. 
     Example 8: Production of scFv Antibody to SFTS Virus 
     Human scFv library was biopanned for the recombinant SFTS virus glycoprotein. After 4 rounds of panning, the antibody clone was screened by enzyme-linked immunosorbent assay analysis (ELISA). It was shown that 10 clones (Ab1 to 5 for Gc and Ab6 to 10 for Gn) recognized the SFTS virus through ELISA. The ELISA analysis result was shown in  FIG. 2 , and the amino acid sequences of each antibody clone were shown in  FIG. 1 . 
     Example 9: Neutralization Activity of Antibody to SFTS Virus 
     The neutralization activity of scFv-hFc antibody purified for the SFTS virus was experimented in Vero cells. Among 10 clones (Ab1 to Ab10) experimented, Ab10 exhibited the strongest neutralization activity. The Ab10 scFv-hFc antibody (100 μl/ml) was diluted 10 folds and titrated for 100 TCID50 SFTS virus (KF358691 strain). The immune fluorescence analysis result and fluorescence strength measurement result of SFTSV infection were shown in  FIG. 3 . 
     In the immune fluorescence analysis (IFA), the cell treated with Ab10(100 μl/ml) exhibited the least virus infection and its neutralization activity was dose-dependent. In other words, the more the amount of MAb 10 to be treated was, the smaller the number of cells infected by SFTS virus was. Compared with MAb 4-5 (positive control), Ab10 showed significantly high neutralization activity. The negative control antibody did not exhibit the neutralization activity at all. 
     This application contains references to amino acid sequences and/or nucleic acid sequences which have been submitted herewith as the sequence listing text file. The aforementioned sequence listing is hereby incorporated by reference in its entirety pursuant to 37 C.F.R. § 1.52(e).