Patent Publication Number: US-2021171941-A1

Title: A primer for next generation sequencer and a method for producing the same, a dna library obtained through the use of a primer for next generation sequencer and a method for producing the same, and a dna analyzing method using a dna library

Description:
TECHNICAL FIELD 
     The present invention relates to a primer used for a next-generation sequencer having an index that enables simultaneous analysis of a plurality of analytes, a method for producing the same, a DNA library using the primer used for a next-generation sequencer, a method for producing the same, and a method of genomic DNA analysis using the DNA library. 
     BACKGROUND ART 
     A next-generation sequencer (NGS) is an apparatus that can read nucleotide sequences of many DNA fragments in parallel. For example, for the use of the next-generation sequencer (Illumina), adaptors are ligated to both ends of each of tens of millions to hundreds of millions of DNA fragments that have been randomly cleaved and 5′ terminuses thereof are immobilized on the flow cell via adaptors. Subsequently, the 5′ terminal adaptor that has been immobilized on the flow cell in advance is annealed to the 3′ terminal adaptor sequence of the DNA fragment to form a bridged DNA fragment. A nucleic acid amplification reaction is carried out with the aid of a DNA polymerase in that state, so that many single-stranded DNA fragments can be topically amplified and immobilized. The next-generation sequencer performs sequencing with the use of the resulting single-stranded DNA as a template. Thus, sequence information as enormous as 40 to 200 Gb can be obtained via single analysis. 
     Sequencing with the use of a next-generation sequencer is carried out by a method in which fluorescence-labeled dNTP uptake is analyzed under a fluorescence microscope. Specifically, dNTP that is blocked with a protective group and fluorescence-labeled at the 3′ terminus is used. dNTP complementary to the single-stranded DNA fragment is incorporated with the aid of DNA polymerase, dNTP is excited with a laser beam, and the fluorescence is read under a fluorescence microscope. The protective group is removed from dNTP, and the subsequent nucleotide is then analyzed in the same manner. Thus, the next-generation sequencer continuously analyzes each nucleotide of the single-stranded DNA immobilized on the flow cell. 
     In particular, according to the next-generation sequencer, an index (it is also referred to as an “index sequence” or a “barcode sequence”) is provided to an adaptor to be ligated to the analyte DNA fragment, so that DNA fragments derived from a plurality of samples can be distinguished from each other. As described above, specifically, enormous sequence information can be obtained via a single analysis, and the origin of the sample from which the sequence information concerning the DNA fragment of interest is derived can be identified with the use of the index sequence included in the sequence information as the indicator. 
     As described in Non-Patent Literature 1, however, analysis with the use of the next-generation sequencer utilizing the index sequence was disadvantageous in terms of a significant variation in the number of reads depending on the index sequence. In Non-Patent Literature 1, however, a difference concerning properties of the index sequence is not systemically analyzed, and accuracy of analysis with the use of the next-generation sequencer using the index sequence was insufficient, disadvantageously. 
     In the past, a method in which universal tail sequences each comprising a different nucleotide sequence is added to each of a pair of primers, multiplex PCR is carried out with the use of the pair of primers, and a group of amplicons applied to the next-generation sequencer (i.e., amplicons with the same index sequence) is obtained has been known (Patent Literature 1). For the purpose of improving the efficiency of analysis of large quantities of samples, in addition, a method of using a pair of primers each containing an adaptor, an index, and a target DNA-specific sequence to prepare a DNA library for the next-generation sequencer has been known (Patent Literature 2). The primer disclosed in Patent Literature 2 is an integrated primer composed of a primer that specifically binds to the target DNA such as a hyper variable region of human mitochondrial DNA, an adaptor primer that is necessary for production of an NGS library, an index primer, and a sequencing primer. 
     CITATION LIST 
     Non Patent Literature 
     NPL 1: David W. Craig et al., Nat. Methods, October 2008; 5 (10): 887-893 
     PATENT LITERATURE 
     PTL 1: US 2016/0326572 A1 
     PTL 2: JP 2017-97935 A 
     SUMMARY OF INVENTION 
     Technical Problem 
     When using a primer used for a next-generation sequencer comprising an index, however, a problem concerning the significantly decreased number of reads depending on the index sequence remains unsolved, and no technique that can dissolve such problem has been known at present. Under the above circumstances, the present invention provides a primer used for a next-generation sequencer that can achieve a large number of reads and a method for producing such primer by elucidating the correlation between the nucleotide sequence of the index and the number of reads. The present invention also provides a DNA library using a primer used for a next-generation sequencer that can achieve a large number of reads, a method for producing such DNA library, and a method of genomic DNA analysis using such DNA library. 
     Solution to Problem 
     The present inventors have conducted concentrated studies in order to solve the above problems. As a result, they elucidated the correlation between the index sequence and the number of reads of the primer used for a next-generation sequencer comprising the index sequence and demonstrated that the number of reads could be deduced based on the nucleotide sequence of the index sequence. They discovered that a primer used for a next-generation sequencer that could achieve a large number of reads can be produced by designing an index sequence that can achieve the putative number of reads exceeding a given level. This has led to the completion of the present invention. 
     The present invention includes the following. 
     (1) A primer used for a next-generation sequencer comprising a nucleotide sequence of 5′-CAAGCAGAAGACGGCATACGAGAT (SEQ ID NO: 1)-N 5 to 15 -GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG (SEQ ID NO: 2)-3′, wherein N 5 to 15  indicates an index sequence of 5 to 15 nucleotides and the index sequence designed as a nucleotide sequence exhibiting a putative number of reads exceeding a given level, which is calculated based on an estimation formula that designates the number of reads as a purpose variable and a type of nucleotide in the index sequence as an explanatory variable. 
     (2) The primer used for a next-generation sequencer according to (1), wherein the index sequence is composed of 8 nucleotides (N: 8; SEQ ID NO: 67). 
     (3) The primer used for a next-generation sequencer according to (1), wherein the estimation formula includes items comprising a type of a nucleotide and a coefficient in accordance therewith concerning the N number of nucleotides constituting the index sequence. 
     (4) The primer used for a next-generation sequencer according to (1), wherein the given level is from 15,000 to 25,000. 
     (5) The primer used for a next-generation sequencer according to (1), which comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 262 to 963. 
     (6) A method for producing a primer used for a next-generation sequencer comprising: a step of calculating, for the primer used for a next-generation sequencer comprising a nucleotide sequence of 5′-CAAGCAGAAGACGGCATACGAGAT (SEQ ID NO: 
     1)-N 5 to 15 -GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG (SEQ ID NO: 
     2)-3′, wherein N 5 to 15  indicates an index sequence of 5 to 15 nucleotides, a putative number of reads based on a nucleotide sequence of the index sequence in accordance with an estimation formula that designates the number of reads as a purpose variable and a type of a nucleotide in the index sequence as an explanatory variable to design a nucleotide sequence as a nucleotide sequence of the index sequence where the calculated putative number of reads exceeds a given level; and a step of synthesizing a nucleotide sequence comprising the index sequence designed in the above step. 
     (7) The method for producing the primer used for a next-generation sequencer according to (6), wherein the index sequence is composed of 8 nucleotides (N: 8; SEQ ID NO: 67). 
     (8) The method for producing the primer used for a next-generation sequencer according to (6), wherein the estimation formula includes items comprising a type of nucleotide and a coefficient in accordance therewith concerning the N number of nucleotides constituting the index sequence. 
     (9) The method for producing the primer used for a next-generation sequencer according to (6), wherein the given level is from 15,000 to 25,000. 
     (10) The method for producing the primer used for a next-generation sequencer according to (6), wherein a sequence of nucleotides 25 to 32 in a nucleotide sequence selected from the group consisting of SEQ ID NOs: 262 to 963 is designed as a nucleotide sequence of the index sequence. 
     (11) A DNA library comprising a DNA fragment comprising, at one end of the analyte DNA, a nucleotide sequence of the primer used for a next-generation sequencer according to any of (1) to (5). 
     (12) The DNA library according to (11), wherein the analyte DNA is a fragment obtained via nucleic acid amplification or a fragment obtained via genomic DNA fragmentation. 
     (13) The DNA library according to (11), wherein the analyte DNA is a fragment obtained by performing a nucleic acid amplification reaction in a reaction solution containing genomic DNA and a random primer at high concentration, and the random primer comprises, at the 5′ terminus, a nucleotide sequence complementary to a nucleotide sequence at the 3′ terminus of the primer used for a next-generation sequencer. 
     (14) A method for producing a DNA library comprising a step of performing a nucleic acid amplification reaction with the use of the primer used for a next-generation sequencer according to any of (1) to (5) above and analyte DNA comprising, at the 5′ terminus, a nucleotide sequence complementary to a nucleotide sequence at the 3′ terminus of the primer used for a next-generation sequencer. 
     (15) The method for producing a DNA library according to (14), wherein the analyte DNA is a fragment obtained via nucleic acid amplification or a fragment obtained via genomic DNA fragmentation. 
     (16) The method for producing a DNA library according to (14), wherein the analyte DNA is a fragment obtained by performing a nucleic acid amplification reaction in a reaction solution containing genomic DNA and a random primer at high concentration, and the random primer comprises, at the 5′ terminus, a nucleotide sequence complementary to a nucleotide sequence at the 3′ terminus of the primer used for a next-generation sequencer. 
     (17) A method of DNA analysis comprising analyzing the DNA library according to any of (11) to (13) using a next-generation sequencer and determining a nucleotide sequence of a DNA fragment contained in the DNA library. 
     Advantageous Effects of Invention 
     The present invention provides a primer used for a next-generation sequencer that can avoid inconvenience, such as a decreased number of reads caused by the index sequence, and provide a large number of reads, a method for producing the same, a DNA library produced with the use of the primer used for a next-generation sequencer, and a method for producing the same. 
     With the use of the DNA library produced using the primer used for a next-generation sequencer according to the present invention, the amount of data (i.e., the number of reads) varying among samples can be prevented, and DNA analysis can be performed with high accuracy. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG. 1  shows a characteristic diagram schematically demonstrating a scheme of an analysis performed with the use of a next-generation sequencer using a primer used for a next-generation sequencer. 
         FIG. 2  shows a characteristic diagram demonstrating a correlation between a type of the index sequence of the primer P7 used for a next-generation sequencer and the number of reads. 
         FIG. 3  shows a characteristic diagram demonstrating a correlation between a type of the index sequence of the primer P5 used for a next-generation sequencer and the number of reads. 
         FIG. 4  shows a characteristic diagram demonstrating a correlation between the putative number of reads determined in accordance with an estimation formula based on types of nucleotides constituting the index sequence of the primer P7 used for a next-generation sequencer and the measured number of reads. 
         FIG. 5  shows a characteristic diagram demonstrating a correlation between the putative number of reads determined in accordance with an estimation formula based on types of nucleotides constituting the index sequence of the primer P5 used for a next-generation sequencer and the measured number of reads. 
     
    
    
     DESCRIPTION OF EMBODIMENTS 
     Hereafter, the present invention is described in greater detail. 
     The primer used for a next-generation sequencer according to the present invention comprises a nucleotide sequence of 5′-CAAGCAGAAGACGGCATACGAGAT (SEQ ID NO: 1)-N 5 to 15 -GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG (SEQ ID NO: 2)-3′, wherein N 5 to 15  indicates an index sequence of 5 to 15 nucleotides. In the nucleotide sequence indicated above, the index sequence indicated as “N 5 to 15 ” is a sequence designed as described in detail below. For example, such sequence can be used as an index for distinguishing samples from each other. Specifically, any index sequence may be used, as long as it is designed in the manner as described below. For example, a different index sequence may be designed for each of a plurality of samples. Thus, sample attribution of the nucleotide sequence analyzed with the use of the next-generation sequencer can be clearly identified on the basis of the index sequence (i.e., multiplex analysis). 
       FIG. 1  schematically shows a scheme of an analysis performed with the use of a next-generation sequencer using a primer used for a next-generation sequencer. The primer used for a next-generation sequencer according to the present invention is based on a primer that is referred to as P7 of the next-generation sequencer (NGS) provided by Illumina. When preparing a DNA library applied to the next-generation sequencer, PCR is carried out using the primer P7 used for a next-generation sequencer and the primer P5 used for a next-generation sequencer. While the primer P5 used for a next-generation sequencer shown in  FIG. 1  comprises an index sequence, the primer may not comprise an index sequence. 
     As shown in  FIG. 1 , a DNA fragment comprising the analyte DNA between the primer P7 used for a next-generation sequencer and the primer P5 used for a next-generation sequencer can be obtained by PCR using the primer P7 used for a next-generation sequencer and the primer P5 used for a next-generation sequencer. A group of DNA fragments obtained by PCR is referred to as a DNA library or a DNA library used for the next-generation sequencer. 
     As shown in  FIG. 1 , the DNA library obtained in the manner described below is analyzed using the next-generation sequencer (Illumina). The next-generation sequencer outputs data of nucleotide sequences concerning many reads as a result of analysis of the DNA library (i.e., the data of nucleotide sequences including the nucleotide sequence of the analyte DNA). 
     Analyte DNA is not particularly limited, and it can be, for example, a DNA fragment derived from genomic DNA derived from a target organism, the genome of which is to be analyzed, DNA prepared for epigenome analysis or DNA prepared for transcript analysis, or an amplification product obtained with the use of genomic DNA as a template (i.e., an amplicon). 
     Index Sequence Design 
     The index sequence of the primer used for a next-generation sequencer according to the present invention is designed in a manner such that a sufficiently large number of reads can be obtained when analyzing the nucleotide sequences of the DNA library with the use of the next-generation sequencer. When designing an index sequence, specifically, an estimation formula that designates the number of reads as a purpose variable and types of nucleotides in the index sequence as an explanatory variable is prepared. In order to prepare such estimation formula, at the outset, a primer used for a next-generation sequencer having an index sequence comprising a particular nucleotide sequence is synthesized, and the number of reads attained with the use of the primer used for a next-generation sequencer is analyzed. Thus, the estimation formula can be calculated on the basis of the nucleotide sequence of the index sequence and the number of reads. 
     When calculating an estimation formula, specifically, various types of algorithms that are generally used for correlational analysis can be adopted, although algorithm is not limited thereto. More specifically, a method of sparse modeling, such as the least absolute shrinkage and selection operator (Lasso), can be adopted. According to the method of L1 regularized estimation among the sparse modeling methods, a coefficient of insignificant parameters can be deduced to be 0, and an estimation formula consisting of adequate parameters can be obtained. 
     For example, an estimation formula includes items including coefficients determined for each nucleotide at a given position in the index sequence and an intercept. By designating particular nucleotides at all the positions in the index sequence, accordingly, the putative number of reads can be determined. With the application of the sparse modeling method, such as LASSO, an estimation formula that designates the coefficient of insignificant parameters; i.e., a nucleotide that is not significantly involved in an increase/decrease in the number of reads, as 0 can be calculated. 
     When designing an index sequence composed of, for example, an index sequence of 8 nucleotides (N: 8); i.e., an index sequence composed of nucleotides 1 to 8 in the 5′ to 3′ direction, more specifically, an estimation formula can be designed to lead to a decreased putative number of reads when nucleotide 1 is adenine or guanine and an increased putative number of reads when it is cytosine. An estimation formula can also be designed to lead to an increased putative number of reads when nucleotide 2 is adenine or thymine and a decreased putative number of reads when nucleotide 2 is guanine. Further, an estimation formula can also be designed to lead to an increased putative number of reads when nucleotide 3 is adenine or thymine and a decreased putative number of reads when nucleotide 3 is guanine. Further, an estimation formula can also be designed to lead to an increased putative number of reads when nucleotide 4 is adenine and a decreased putative number of reads when nucleotide 4 is cytosine or guanine. Further, an estimation formula can also be designed to lead to an increased putative number of reads when nucleotide 5 is adenine and a decreased putative number of reads when nucleotide 5 is guanine. Further, an estimation formula can also be designed to lead to an increased putative number of reads when nucleotide 6 is cytosine and a decreased putative number of reads when nucleotide 6 is thymine. Further, an estimation formula can also be designed to lead to a decreased putative number of reads when nucleotide 7 is adenine and an increased putative number of reads when nucleotide 7 is guanine. Further, an estimation formula can also be designed to lead to a decreased putative number of reads when nucleotide 8 is guanine and an increased putative number of reads when nucleotide 8 is thymine. 
     In particular, an estimation formula can be designed to result in a putative number of reads decreased to a significant extent when nucleotide 1 is adenine or guanine, nucleotide 2 is guanine, and nucleotide 8 is guanine. In addition, an estimation formula can be designed to result in a putative number of reads increased to a significant extent when nucleotide 2 is adenine or thymine, nucleotide 3 is adenine, and nucleotide 5 is adenine. 
     More specifically, a coefficient for each type of a nucleotide in the index sequence and an intercept can be designated as shown in the table below. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 1 
               
               
                   
                   
               
             
            
               
                   
                 Intercept 
                 81720.7 
               
               
                   
                 A1 
                 −65033.1 
               
               
                   
                 C1 
                 1326.4 
               
               
                   
                 G1 
                 −16997.0 
               
               
                   
                 T1 
                 0.0 
               
               
                   
                 A2 
                 10936.3 
               
               
                   
                 C2 
                 0.0 
               
               
                   
                 G2 
                 −12399.2 
               
               
                   
                 T2 
                 11712.9 
               
               
                   
                 A3 
                 12112.2 
               
               
                   
                 C3 
                 0.0 
               
               
                   
                 G3 
                 −623.5 
               
               
                   
                 T3 
                 5964.4 
               
               
                   
                 A4 
                 6884.5 
               
               
                   
                 C4 
                 −5664.4 
               
               
                   
                 G4 
                 −6049.9 
               
               
                   
                 T4 
                 0.0 
               
               
                   
                 A5 
                 9257.0 
               
               
                   
                 C5 
                 0.0 
               
               
                   
                 G5 
                 −6210.8 
               
               
                   
                 T5 
                 0.0 
               
               
                   
                 A6 
                 0.0 
               
               
                   
                 C6 
                 −644.0 
               
               
                   
                 G6 
                 0.0 
               
               
                   
                 T6 
                 3.2 
               
               
                   
                 A7 
                 −3575.9 
               
               
                   
                 C7 
                 0.0 
               
               
                   
                 G7 
                 1013.1 
               
               
                   
                 T7 
                 0.0 
               
               
                   
                 A8  
                 0.0 
               
               
                   
                 C8 
                 0.0 
               
               
                   
                 G8 
                 −8607.7 
               
               
                   
                 T8 
                 6490.3 
               
               
                   
                   
               
            
           
         
       
     
     In Table 1, “A1” indicates an embodiment in which nucleotide 1 in the 5′ to 3′ direction of the index sequence is adenine. Each of other notations also indicates a position in the 5′ to 3′ direction of the index sequence and a type of nucleotide. 
     With the use of the estimation formula shown in the table above, for example, the putative number of reads obtained when a particular nucleotide sequence is designated for an index sequence can be determined. The estimation formulae prepared in the manner described above are not limited to those exemplified in Table 1, and the results attained with the formulae are highly correlated with the measured value (correlational coefficient: 0.9 or higher). By calculating the putative number of reads for each nucleotide sequence of an index sequence with the use of the estimation formula and selecting a nucleotide sequence exhibiting a putative number of reads exceeding a given level, a large number of reads can be obtained. 
     When designing an index sequence, the putative number of reads designated as a threshold is not particularly limited, and it can be adequately determined in accordance with a type of data analysis of interest. For example, a threshold of the putative number of reads can be set in a range from 15,000 to 25,000, preferably from 17,000 to 23,000, more preferably from 19,000 to 21,000, and further preferably from 19,500 to 20,500. As described in detail in the examples below, a group of nucleotide sequences with a significantly small number of reads is identified for the nucleotide sequence of the index sequence. The putative number of reads of the group of nucleotide sequences was calculated and found to be about 20,000 and specifically 20,051.8 at a maximum. By adjusting the threshold to about 20,000, for example, 20,052, accordingly, an index sequence that can actually obtain a large number of reads can be designed. 
     With the use of the estimation formula shown in Table 1, the nucleotide sequence of the primer used for a next-generation sequencer comprising an index sequence with the putative number of reads exceeding 20,052 was identified (described in detail in the examples below). A specific example of the primer used for a next-generation sequencer according to the present invention is a nucleotide sequence selected from the group consisting of SEQ ID NOs: 262 to 963. 
     Application to Next-Generation Sequencing 
     A nucleic acid amplification reaction is carried out with the use of the primer used for a next-generation sequencer according to the present invention described above. Thus, a DNA library used for a next-generation sequencer (i.e., a DNA library for a next-generation sequencer) can be prepared. Specifically, PCR is carried out in a reaction solution containing the primer P7 used for a next-generation sequencer according to the present invention, analyte DNA, and the primer P5 used for a next-generation sequencer, as shown in  FIG. 1 . Analyte DNA comprises, at both terminuses, a sequence that is in common with the primer P7 used for a next-generation sequencer and a sequence that is in common with the primer P5 used for a next-generation sequencer, respectively. In the nucleic acid amplification reaction, accordingly, the primers P7 and P5 used for a next-generation sequencer are annealed to the both terminuses of analyte DNA, and a nucleic acid amplification reaction with the use of analyte DNA as a template proceeds. As a result, a group of nucleic acid fragments (a DNA library) comprising the primer P5 used for a next-generation sequencer, analyte DNA, and the primer P7 used for a next-generation sequencer in that order can be obtained, as shown in  FIG. 1 . 
     Since the resulting DNA library comprises, at both terminuses, the primers P5 and P7 used for a next-generation sequencer, it can be applied to the next-generation sequencer (Illumina). In addition, the resulting DNA library comprises the primer P7 used for a next-generation sequencer according to the present invention. Accordingly, a larger number of reads can be analyzed with the use of the next-generation sequencer. Specifically, the number of reads close to the putative number of reads determined in accordance with the estimation formula described above can be analyzed. 
     The next-generation sequencer performs sequencing by amplifying the target DNA on the flow cell via bridge PCR and the sequencing-by-synthesis method while conducting synthesis. 
     A nucleic acid amplification reaction can be performed with the use of a reaction solution containing the primer P7 used for a next-generation sequencer according to the present invention, analyte DNA, and the primer P5 used for a next-generation sequencer without particular limitation. General conditions for a nucleic acid amplification reaction can be adopted. For example, the reaction solution contains analyte DNA as a template, the primers P5 and P7 used for a next-generation sequencer, DNA polymerase, deoxyribonucleotide triphosphate (dNTP; i.e., a mixture of dATP, dCTP, dTTP, and dGTP) as a substrate, and a buffer. 
     In particular, the concentration of the primers P5 and P7 used for a next-generation sequencer can be 0.01 to 5.0 microM, preferably 0.1 to 2.5 microM, and most preferably 0.3 to 0.7 microM. 
     While the amount of analyte DNA used as a template in the nucleic acid amplification reaction is not particularly limited, it is preferably 0.1 to 1000 ng, more preferably 1 to 500 ng, further preferably 5 to 200 ng, and most preferably 10 to 100 ng, when the amount of the reaction solution is 50 microliters. 
     A method for preparing an analyte DNA fragment serving as a template is not particularly limited. A reaction solution after the completion of the nucleic acid amplification reaction using the random primer described in detail below may be used without any processing. Alternatively, analyte DNA may be purified from the reaction solution and used. 
     Also, a type of DNA polymerase, a concentration of deoxyribonucleotide triphosphate as a substrate (dNTP; i.e., a mixture of dATP, dCTP, dTTP, and dGTP), a buffer composition, and thermal cycling conditions adopted for the nucleic acid amplification reaction can be in accordance with the conditions adopted for general nucleic acid amplification reactions. A nucleic acid amplification reaction involving the use of a primer used for a next-generation sequencer may be carried out by the hot start method, or amplified fragments may be obtained via a nucleic acid amplification reaction. 
     As described above, a DNA library applicable to the next-generation sequencing apparatus can be prepared with the use of a first DNA fragment obtained with the use of a random primer as a template and a second DNA fragment amplified with the use of the next-generation sequencer. 
     Analyte DNA 
     An example of analyte DNA is an amplified product (amplicon) obtained from genomic DNA or the like as a template. An amplified product can be obtained by, for example, performing a nucleic acid amplification reaction in a reaction solution containing a primer having an arbitrary nucleotide sequence (hereafter referred to as a “random primer”) at high concentration. When a primer concentration is high herein, such concentration is higher than a concentration of the primer used in a conventional nucleic acid amplification reaction. Specifically, the method involves the use of a random primer at concentration higher than that of the primer used in a conventional nucleic acid amplification reaction. A template contained in the reaction solution can be genomic DNA prepared from an analyte organism. 
     In the method, a target organism species is not limited, and any organism species, such as animals including humans, plants, microorganisms, and viruses, can be targets. According to the method, specifically, many amplified products can be obtained from any organism species as analyte DNAs. 
     According to the method, nucleic acid fragments (a group of nucleic acid fragments) can be amplified with high reproducibility by defining the random primer concentration as described above. The term “reproducibility” used herein refers to a degree of consistency among nucleic acid fragments amplified as a result of nucleic acid amplification reactions performed a plurality of times with the use of the same template and the same random primer. Specifically, the term “high reproducibility” (or when reproducibility is high) herein refers to a high degree of consistency among nucleic acid fragments amplified as a result of nucleic acid amplification reactions performed a plurality of times with the use of the same template and the same random primer. 
     A degree of reproducibility can be determined by, for example, performing nucleic acid amplification reactions a plurality of times with the use of the same template and the same random primer, subjecting the amplified fragments to electrophoresis, calculating the Spearman&#39;s rank correlation coefficient of the obtained fluorescence unit (FU), and evaluating the degree of reproducibility based on the coefficient. The Spearman&#39;s rank correlation coefficient is generally represented by the symbol p (rho). For example, an experiment can be evaluated as reproducible when rho is greater than 0.9. 
     The sequence of a random primer that can be used in the method is not limited. For example, a nucleotide sequence of 9 to 30 nucleotides can be used. In particular, a random primer is composed of an arbitrary sequence of 9 to 30 nucleotides, and a type of nucleotide (a type of sequence) is not particularly limited. A random primer is composed of one or more types of nucleotides, preferably 1 to 10,000 types of nucleotides, more preferably 1 to 1,000 types of nucleotides, further preferably 1 to 100 types of nucleotides, and most preferably 1 to 96 types of nucleotides. With the use of a random primer composed of the number of nucleotides described above (a group of nucleotides), amplified nucleic acid fragments can be obtained with higher reproducibility. When a random primer is composed of a plurality of nucleotide sequences, all the nucleotide sequences are not necessarily composed of the same number of nucleotides (9 to 30 nucleotides), and the sequence may comprise a plurality of nucleotide sequences of different lengths. 
     In order to obtain a particular amplicon by means of the nucleic acid amplification reaction, in general, a nucleotide sequence of a primer is designed in accordance with the amplicon. For example, a pair of primers is designed to sandwich a position corresponding to an amplicon in the template DNA such as genomic DNA. In this case, primers are designed to hybridize to a particular region in the template. Thus, primers can be referred to as “specific primers.” 
     Unlike a primer that is designed to obtain a particular amplicon, in contrast, a random primer is not designed to hybridize to a particular region in template DNA, but it is designed to obtain a random amplicon. A random primer may comprise an arbitrary nucleotide sequence, and it can be involved in random amplicon amplification by incidentally hybridizing to a complementary region in the template DNA. 
     Specifically, a random primer can be composed of an arbitrary nucleotide sequence involved in random amplicon amplification, as described above. An arbitrary sequence is not particularly limited. For example, a nucleotide sequence of nucleotides selected randomly from the group consisting of adenine, guanine, cytosine, and thymine may be designed, or a specific nucleotide sequence may be designed. Examples of specific nucleotide sequences include a nucleotide sequence comprising a restriction enzyme recognition sequence or a nucleotide sequence comprising an adaptor sequence used for the next-generation sequencer. 
     When a plurality of types of nucleotides are designed as random primers, a plurality of nucleotide sequences of given lengths can be designed by randomly selecting nucleotides from the group consisting of adenine, guanine, cytosine, and thymine. When a plurality of types of nucleotides are designed as random primers, a plurality of nucleotide sequences composed of common regions of particular nucleotide sequences and un-common regions of arbitrary nucleotide sequences can be designed. An un-common region may be composed of a nucleotide sequence of nucleotides randomly selected from the group consisting of adenine, guanine, cytosine, and thymine, 4 types of nucleotides in combination (i.e., adenine, guanine, cytosine, and thymine), or some of nucleotides selected from among the 4 types of nucleotides described above in combination. A common region is not particularly limited, and it may be composed of any nucleotide sequence. For example, a common region can be composed of a nucleotide sequence comprising a restriction enzyme recognition sequence, a nucleotide sequence comprising an adaptor sequence applied to the next-generation sequencer, or a nucleotide sequence common among a particular gene family. 
     When a plurality of nucleotide sequences of given lengths are designed as random primers by randomly selecting nucleotides from among the four types of nucleotides, such nucleotide sequences are preferably designed in such a manner that 30% or more, preferably 50% or more, more preferably 70% or more, and further preferably 90% or more of the full-length sequences exhibit 70% or lower, more preferably 60% or lower, further preferably 50% or lower, and most preferably 40% or lower identity. Thus, amplified fragments can be obtained over the entire genomic DNA of the target organism species. Specifically, homogeneity among amplified fragments can be improved. 
     When a plurality of nucleotide sequences each composed of a common region of a particular nucleotide sequence and an un-common region of an arbitrary nucleotide sequence are designed as a plurality of random primers, for example, each sequence can be designed to comprise a 3′ terminal region of several nucleotides as an un-common region and a remaining 5′ terminal region as a common region. By designating a 3′-terminal region of the “n” number of nucleotides as an un-common region, 4 n  types of random primers can be designed. The “n” number can be 1 to 5, preferably 2 to 4, and more preferably 2 or 3. 
     As a random primer comprising a common region and an un-common region, for example, a total of 16 types of random primers each comprising a 5′ terminal region as an adaptor sequence applied to the next-generation sequencer (i.e., a common region) and a 3′-terminal region of 2 nucleotides (i.e., an un-common region) can be designed. If a 3′ terminal region is of 3 nucleotides (an un-common region), a total of 64 types of random primers can be designed. As types of random primers are increased, amplified fragments can be more extensively obtained over the entire genomic DNA of the target organism species. When designing a random primer comprising a common region and an un-common region, accordingly, a 3′ terminal region is preferably composed of 3 nucleotides. 
     After 64 types of nucleotide sequences each composed of a common region and an un-common region of 3 nucleotides are designed, for example, up to 63 types of random primers selected from among the 64 types of nucleotide sequences may be used. In other words, more satisfactory results of analysis may occasionally be obtained via nucleic acid amplification reactions or with the use of the next-generation sequencer with the use of up to 63 types of random primers, in comparison with the use of all the 64 types of random primers. When 64 types of random primers are used, specifically, the number of reads of a particular nucleic acid fragment may occasionally be increased to a significant extent. In such a case, more satisfactory results of analysis can be obtained with the use of up to 63 types of random primers selected from among the 64 types of random primers while excluding one or more random primers involved in amplification of the particular nucleic acid fragment. 
     When 16 types of random primers each composed of a common region and an un-common region of 2 nucleotides are designed, also, more satisfactory results of analysis can occasionally be obtained via nucleic acid amplification reactions or with the use of the next-generation sequencer when up to 15 types of random primers selected from among the 16 types of random primers are used. 
     A nucleotide sequence used as a random primer is preferably designed to adjust GC content within a range of 5% to 95%, more preferably within a range of 10% to 90%, further preferably within a range of 15% to 80%, and most preferably within a range of 20% to 70%. With the use of the nucleotide sequence with GC content within the range described above as a random primer, an amplified nucleic acid fragment can be obtained with higher reproducibility. GC content is a proportion of guanine and cytosine relative to the entire nucleotide chain. 
     A nucleotide sequence used as a random primer is preferably designed to adjust the length of continuous nucleotides to 80% or less, more preferably 70% or less, further preferably 60% or less, and most preferably 50% or less, relative to the full-length sequence. Alternatively, a nucleotide sequence used as a random primer is preferably designed to adjust the number of continuous nucleotides to 8 or less, more preferably 7 or less, further preferably 6 or less, and most preferably 5 or less. With the use of the nucleotide sequence with the number of continuous nucleotides within the range described above as a random primer, an amplified nucleic acid fragment can be obtained with higher reproducibility. 
     A nucleotide sequence used as a random primer is preferably designed to refrain from comprising a complementary region of 6 nucleotides or more, more preferably 5 nucleotides or more, and further preferably 4 nucleotides or more in a molecule. Thus, formation of a double strand in the molecule can be prevented, and an amplified nucleic acid fragment can be obtained with higher reproducibility. 
     When designing a plurality of types of nucleotide sequences as random primers, further, each nucleotide sequence is preferably designed to refrain from comprising a complementary region of 6 nucleotides or more, more preferably 5 nucleotides or more, and further preferably 4 nucleotides or more. Thus, formation of a double strand between nucleotides can be prevented, and an amplified nucleic acid fragment can be obtained with higher reproducibility. 
     When designing a plurality of types of nucleotide sequences as random primers, in addition, it is preferable that each nucleotide sequence is designed in such a manner that 3′-terminal sequences of 6 nucleotides or more, preferably 5 nucleotides or more, and more preferably 4 nucleotides or more refrain from being complementary to each other. Thus, double strand formation between nucleotides can be prevented, and an amplified nucleic acid fragment can be obtained with higher reproducibility. 
     The term “complementary region” or “complementary sequence” refers to, for example, a region or sequence having identity of 80% to 100% (e.g., regions or sequences of 4 or 5 nucleotides in regions of 5 nucleotides are complementary to each other) or a region or sequence having identity of 90% to 100% (e.g., regions or sequences of 5 nucleotides in regions of 5 nucleotides are complementary to each other). 
     A nucleotide sequence used as a random primer is preferably designed to have a Tm value suitable for the thermal cycling conditions in the nucleic acid amplification reaction (the annealing temperature, in particular). A Tm value can be calculated by a known method of calculation, such as nearest-neighbor base paring, the Wallace method, or the GC content percentage method, although the method is not particularly limited thereto. Specifically, a nucleotide sequence used as a random primer is preferably designed to have a Tm value of 10 to 85 degrees C., more preferably 12 to 75 degrees C., further preferably 14 to 70 degrees C., and most preferably 16 to 65 degrees C. Thus, an amplified nucleic acid fragment can be obtained with higher reproducibility under given thermal cycling conditions (a given annealing temperature, in particular) in the nucleic acid amplification reaction. 
     When designing a plurality of types of nucleotide sequences as random primers, nucleotide sequences are preferably designed in such a manner that a variation in Tm values among a plurality of nucleotide sequences is 50 degrees C. or lower, preferably 45 degrees C. or lower, more preferably 40 degrees C. or lower, and most preferably 35 degrees C. or lower. Thus, an amplified nucleic acid fragment can be obtained with higher reproducibility under given thermal cycling conditions (a given annealing temperature, in particular) in the nucleic acid amplification reaction. 
     According to the method, many amplified fragments are obtained via nucleic acid amplification reactions involving the use of the random primers and genomic DNA as a template. In the nucleic acid amplification reaction, in particular, the random primer concentration in the reaction solution is adjusted to be higher than that used in a conventional nucleic acid amplification reaction. Thus, many amplified fragments can be obtained with the use of genomic DNA as a template while achieving high reproducibility. 
     In the nucleic acid amplification reaction, an amplified fragment is synthesized in a reaction solution containing genomic DNA as a template, the random primers described above, DNA polymerase, deoxyribonucleotide triphosphate as a substrate (dNTP; a mixture of dATP, dCTP, dTTP, and dGTP), and a buffer under the given thermal cycling conditions. In the nucleic acid amplification reaction, the reaction solution is required to contain Mg 2+  at a given concentration, and the buffer in the composition described above contains MgCl 2 . When the buffer does not contain MgCl 2 , the reaction solution should contain MgCl 2  in addition to the components described above. 
     In the nucleic acid amplification reaction, it is preferable that the random primer concentration be adequately determined in accordance with the nucleotide length of the random primer. When a plurality of types of nucleotide sequences of different nucleotide lengths are used as random primers, the average nucleotide length may be adopted as the nucleotide length of the random primer (it may be a simple average or weighted average including the amount of nucleotides). 
     Specifically, the nucleic acid amplification reaction is carried out with the use of a random primer of 9 to 30 nucleotides while adjusting the random primer concentration at 4 to 200 microM, and preferably 4 to 100 microM. By conducting the nucleic acid amplification reaction under such conditions, many amplified fragment, and, in particular, many amplified fragments of 100 to 500 nucleotides, can be obtained while achieving high reproducibility. 
     The amount of genomic DNA serving as a template in the nucleic acid amplification reaction is not particularly limited, and it is preferably 0.1 to 1,000 ng, more preferably 1 to 500 ng, further preferably 5 to 200 ng, and most preferably 10 to 100 ng, when the amount of the reaction solution is 50 microliters. Thus, an amplification reaction with the use of a random primer is not inhibited, and many amplified fragments can be obtained while achieving high reproducibility. 
     A method for preparing genomic DNA is not particularly limited, and a conventional method can be adopted. With the use of a commercialized kit, genomic DNA can be easily prepared from the target organism species. Genomic DNA extracted from an organism by a conventional technique or with the use of a commercialized kit may be used without further processing. Alternatively, genomic DNA extracted from an organism and purified or genomic DNA subjected to restriction enzyme treatment or ultrasonic treatment may be used. 
     DNA polymerase used in the nucleic acid amplification reaction is not particularly limited, and an enzyme having DNA polymerase activity under thermal cycling conditions for the nucleic acid amplification reaction can be used. Specifically, thermostable DNA polymerase used for a general nucleic acid amplification reaction can be used. Examples of DNA polymerases include DNA polymerase derived from thermophilic bacteria, such as Taq DNA polymerase, and DNA polymerase derived from ultrathermophilic Archaea, such as KOD DNA polymerase or Pfu DNA polymerase. In the nucleic acid amplification reaction, in particular, use of Pfu DNA polymerase in combination with the random primer is preferable. With the use of such DNA polymerase, many amplified fragments can be obtained with higher certainty while achieving high reproducibility. 
     In the nucleic acid amplification reaction, the concentration of deoxyribonucleotide triphosphate serving as a substrate (dNTP; i.e., a mixture of dATP, dCTP, dTTP, and dGTP) is not particularly limited, and it can be 5 microM to 0.6 mM, preferably 10 microM to 0.4 mM, and more preferably 20 microM to 0.2 mM. Thus, errors caused by erroneous incorporation by DNA polymerase can be prevented, and many amplified fragments can be obtained while achieving high reproducibility. 
     A buffer used in the nucleic acid amplification reaction is not particularly limited, and a buffer contains MgCl 2  as described above. An example thereof is a solution containing Tris-HCl (pH 8.3) and KCl. While the concentration of Mg 2+  is not particularly limited, for example, it can be 0.1 to 4.0 mM, preferably 0.2 to 3.0 mM, more preferably 0.3 to 2.0 mM, and further preferably 0.5 to 1.5 mM. Thus, many amplified fragments can be obtained while achieving high reproducibility. 
     The thermal cycling conditions in the nucleic acid amplification reaction are not particularly limited, and general thermal cycling conditions can be adopted. For example, a thermal cycle is composed of: the initial thermal denaturation by which genomic DNA as a template is dissociated into single strands; repetition of a “thermal denaturation, annealing, and extension” cycle a plurality of times (e.g., 20 to 40 times); and optional extension for a given period of time; followed by storage in the end. 
     Thermal denaturation can be carried out at, for example, 93 to 99 degrees C., preferably 95 to 98 degrees C., and more preferably 97 to 98 degrees C. Annealing temperature varies depending on a Tm value of the random primer, and it can be, for example, 30 to 70 degrees C., preferably 35 to 68 degrees C., and more preferably 37 to 65 degrees C. Extension can be carried out at, for example, 70 to 76 degrees C., preferably 71 to 75 degrees C., and more preferably 72 to 74 degrees C. Storage can be carried out at, for example, 4 degrees C. 
     The initial thermal denaturation can be carried out within the temperature range described above for a period of time of, for example, 5 seconds to 10 minutes, preferably 10 seconds to 5 minutes, and more preferably 30 seconds to 2 minutes. Thermal denaturation in the “thermal denaturation, annealing, and extension” cycle can be carried out within the temperature range described above for a period of time of, for example, 2 seconds to 5 minutes, preferably 5 seconds to 2 minutes, and more preferably 10 seconds to 1 minute. Annealing in the “thermal denaturation, annealing, and extension” cycle can be carried out within the temperature range described above for a period of time of, for example, 1 second to 3 minutes, preferably 3 seconds to 2 minutes, and more preferably 5 seconds to 1 minute. Extension in the “thermal denaturation, annealing, and extension” cycle can be carried out within the temperature range described above for a period of time of, for example, 1 second to 3 minutes, preferably 3 seconds to 2 minutes, and more preferably 5 seconds to 1 minute. 
     In the method, an amplified fragment may be obtained via a nucleic acid amplification reaction involving the hot-start method. The hot-start method avoids a non-specific amplification caused by mispriming or primer-dimer formation before the “thermal denaturation, annealing, and extension” cycle. The hot-start method uses an enzyme with the DNA polymerase activity thereof being suppressed by binding an anti-DNA polymerase antibody to the enzyme or via chemical modification thereof. Thus, DNA polymerase activity is suppressed, and a non-specific reaction before the thermal cycle can be avoided. In the hot-start method, temperature is set at a high level in the first thermal cycle, DNA polymerase activity is thus restored, and the subsequent nucleic acid amplification reaction proceeds. 
     As described above, the nucleic acid amplification reaction is performed with the use of the random primer of 9 to 30 nucleotides by adjusting the random primer concentration to 4 to 200 microM in the reaction solution. Thus, many amplified fragments can be obtained with the use of genomic DNA as a template and the random primer. The nucleic acid amplification reaction can be performed with very high reproducibility when the random primer of 9 to 30 nucleotides is used and the random primer concentration is adjusted to 4 to 200 microM. By performing the nucleic acid amplification reaction described above, specifically, many amplified fragments can be obtained while achieving very high reproducibility. 
     Also, the nucleic acid amplification reaction is performed with the use of the random primer of 9 to 30 nucleotides by adjusting the random primer concentration to 4 to 200 microM in the reaction solution. Thus, in particular, many amplified fragments of about 100 to 500 nucleotides can be obtained with the use of genomic DNA as a template. Such many amplified fragments of about 100 to 500 nucleotides are suitable for mass-analysis of nucleotide sequences with the use of, for example, the next-generation sequencer in terms of size, and sequence information can be obtained with high accuracy. According to the present invention, specifically, DNA fragments of about 100 to 500 nucleotides can be prepared. 
     Further, the nucleic acid amplification reaction is performed with the use of the random primer of 9 to 30 nucleotides by adjusting the random primer concentration to 4 to 200 microM in the reaction solution. Thus, amplified fragments can be obtained uniformly over the entire genomic DNA. In other words, DNA fragments are amplified throughout the genome instead of a given exclusive region of genomic DNA by the nucleic acid amplification reaction using the random primer. 
     After the completion of the nucleic acid amplification reaction with the use of the random primer, the resulting amplified fragments can be subjected to restriction enzyme treatment, size selection treatment, sequence capture treatment, or other treatment. Thus, particular amplified fragments (i.e., a fragment comprising a particular restriction enzyme site, an amplified fragment of a particular size, or an amplified fragment comprising a particular sequence) can be obtained from among the resulting amplified fragments. 
     Method of Genomic DNA Analysis 
     With the use of the amplicon prepared in the manner described above, genomic DNA analysis, such as genotype analysis, can be performed. The amplicon prepared in the manner described above has very high reproducibility, a size thereof is suitable for the next-generation sequencer, and it is uniform throughout the genome. Accordingly, the amplicon prepared in the manner described above can be used as a DNA marker (it is also referred to as a “genetic marker” or “gene marker”). The term “DNA marker” used herein extensively refers to a characteristic nucleotide sequence existing within genomic DNA. A DNA marker can be a nucleotide sequence in the genome that serves as a marker concerning genetic traits. A DNA marker can be used for, for example, genotype identification, linkage mapping, gene mapping, breeding comprising a step of selection using a marker, back cross using a marker, mapping of a quantitative trait locus, bulk segregant analysis, variety identification, or linkage disequilibrium mapping. 
     With the use of the primer used for a next-generation sequencer according to the present invention, in particular, the nucleotide sequence of the amplicon prepared in the manner described above is determined with the use of the next-generation sequencer or the like, and the presence or absence of the DNA marker can be determined on the basis of the resulting nucleotide sequence. 
     For example, the presence or absence of the DNA marker can be determined on the basis of the number of reads of the nucleotide sequence. The next-generation sequencer is not particularly limited, and it is a nucleotide sequencing apparatus that is capable of utilizing the primer used for a next-generation sequencer according to the present invention, that is also referred to as the “second-generation sequence,” and that is capable of simultaneous and parallel determination of nucleotide sequences of tens of millions of DNA fragments. The sequencing principle of the next-generation sequencer is not particularly limited. For example, target DNA is amplified on the flow cell via bridge PCR and sequencing-by-synthesis, and sequencing is performed with synthesis. Specific examples of the next-generation sequencer include MiniSeq, MiSeq, NextSeq, HiSeq, and HiSeq X Series (Illumina). 
     Alternatively, the nucleotide sequence of the amplicon prepared in the manner described above is compared with a reference nucleotide sequence. Thus, the presence or absence of the DNA marker can be determined. A reference nucleotide sequence is a known standard sequence, such as a known sequence stored in a database. Specifically, an amplicon of a given organism is prepared as described above, the nucleotide sequence thereof is determined, and the nucleotide sequence is compared with a reference nucleotide sequence. A nucleotide sequence that is different from the reference nucleotide sequence can be determined as a DNA marker associated with the given organism (i.e., a characteristic nucleotide sequence existing within genomic DNA). The identified DNA marker can be further analyzed in accordance with a conventional technique, and the correlation thereof with a genetic trait (a phenotype) can be determined. Specifically, a DNA marker associated with a phenotype (it is occasionally referred to as a “selection marker”) can be identified from among the DNA markers identified in the manner described above. 
     In addition, the nucleotide sequence of the amplicon prepared in the manner described above is compared with the nucleotide sequence of the amplicon prepared with the use of genomic DNA derived from another organism or another tissue. Thus, the presence or absence of the DNA marker can be determined. Specifically, amplicons of two or more organisms or two different tissues are prepared as described above, nucleotide sequences thereof are determined, and the nucleotide sequences thereof are compared. The nucleotide sequence determined to be different can be designated as a DNA marker (a characteristic nucleotide sequence existing within genomic DNA) associated with the tested organism or tissue. The identified DNA marker can be further analyzed in accordance with a conventional technique, and the correlation thereof with a genetic trait (a phenotype) can be determined. Specifically, a DNA marker associated with a phenotype (it is occasionally referred to as a “selection marker”) can be identified from among the DNA markers identified in the manner described above. 
     The nucleotide sequence information analyzed with the use of the primer used for a next-generation sequencer according to the present invention as described above can be used for various types of analyses, such as metagenomics analysis that investigates diversity of microorganisms, genomic mutation analysis of somatic cells such as tumor tissues, genotype analysis utilizing microarrays, ploidy analysis, calculation of the chromosome number, analysis of increase/decrease in the chromosome number, analysis of partial insertion, deletion, replication, or translocation of the chromosome, analysis of foreign genome inclusion, parentage diagnosis, or hybrid seed purity ex-amination. 
     EXAMPLES 
     Hereafter, the present invention is described in greater detail with reference to the examples, although the technical scope of the present invention is not limited to the following examples. 
     Example 1 
     In this example, the primers P7 and P5 used for the next-generation sequencer (Illumina) were analyzed in terms of the correlation between the index sequence and the amount of the data obtained with the use of the next-generation sequencer, and primers suitable for analysis using the next-generation sequencer were developed. 
     1. Materials 
     In this example, genomic DNA extracted from the rice variety Nipponbare using the DNeasy Plant Mini kit (QIAGEN) and purified was used. 
     2. Method 
     2.1 Design of Random Primer 
     As random primers, 63 types of nucleotide sequences each composed of a total of 13 nucleotides: i.e., 10 nucleotides (GTTACACACG) at the 3′ terminus of the Nextera adapter sequence for the next-generation sequencer (Illumina) and arbitrary 3 nucleotides except for TGC added to the 3′ terminus of the 10-nucleotide sequence, were designed (Table 2). 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 2 
               
               
                   
                   
               
               
                   
                 Random primer 
                 SEQ ID NO: 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 TAAGAGACAGAAA 
                 3 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGAAC 
                 4 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGAAG 
                 5 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGAAT 
                 6 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGACA 
                 7 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGACC 
                 8 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGACG 
                 9 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGACT 
                 10 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGAGA 
                 11 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGAGC 
                 12 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGAGG 
                 13 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGAGT 
                 14 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGATA 
                 15 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGATC 
                 16 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGATG 
                 17 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGATT 
                 18 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCAA 
                 19 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCAC 
                 20 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCAG 
                 21 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCAT 
                 22 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCCA 
                 23 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCCC 
                 24 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCCG 
                 25 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCCT 
                 26 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCGA 
                 27 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCGC 
                 28 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCGG 
                 29 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCGT 
                 30 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCTA 
                 31 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCTC 
                 32 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCTG 
                 33 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGCTT 
                 34 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGAA 
                 35 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGAC 
                 36 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGAG 
                 37 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGAT 
                 38 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGCA 
                 39 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGCC 
                 40 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGCG 
                 41 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGCT 
                 42 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGGA 
                 43 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGGC 
                 44 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGGG 
                 45 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGGT 
                 46 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGTA 
                 47 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGTC 
                 48 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGTG 
                 49 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGGTT 
                 50 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTAA 
                 51 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTAC 
                 52 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTAG 
                 53 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTAT 
                 54 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTCA 
                 55 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTCC 
                 56 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTCG 
                 57 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTCT 
                 58 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTGA 
                 59 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTGG 
                 60 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTGT 
                 61 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTTA 
                 62 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTTC 
                 63 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTTG 
                 64 
               
               
                   
                   
               
               
                   
                 TAAGAGACAGTTT 
                 65 
               
               
                   
                   
               
            
           
         
       
     
     3.2 Preparation of Analyte DNA 
     To the rice-derived genomic DNA (30 ng) described in 1. above (Materials), a 0.2 mM dNTP mixture, 1.0 mM MgCl 2 , 1.25 units of DNA polymerase (PrimeSTAR, TAKARA), and a random primer (final concentration; 40 microM) were added, and a reaction solution was prepared while adjusting the final reaction level to 50 microliters. The resultant was subjected to PCR under thermal cycling conditions comprising 98 degrees C. for 2 minutes and 30 cycles of 98 degrees C. for 10 seconds, 50 degrees C. for 15 seconds, and 72 degrees C. for 20 seconds, followed by storage at 4 degrees C. Thus, many analyte DNAs were prepared with the use of the random primer and the rice-derived genomic DNA as a template. 
     3.3. Preparation of DNA Library for Next-Generation Sequencer 
     To the analyte DNA (1 microliter) prepared in 3.2 above, a 0.2 mM dNTP mixture, 1.0 mM MgCl 2 , 1.25 units of DNA polymerase (PrimeSTAR, TAKARA), and 0.25 microM each of the primers P7 and P5 used for a next-generation sequencer were added, and a reaction solution (50 microliters) was prepared. 
     The primers P7 and P5 used for a next-generation sequencer were prepared based on the sequence information of the Nextera adaptor (Illumina) shown in Table 3. In Table 3 below, 8 nucleotides indicated by asterisks constitute an index sequence. The index sequence of P5 is a reverse complementary sequence of the index sequence of P7. 
     
       
         
           
               
               
               
             
               
                 TABLE 3  
               
               
                   
               
               
                 Primer 
                 Sequence * 
                 SEQ ID NO: 
               
               
                   
               
             
            
               
                 Primer P5 for  
                 AATGATACGGCGACCACCGA 
                 66 
               
               
                 next-generation 
                 GATCTACAC******** 
                   
               
               
                 sequencer 
                 TCGTCGGCAGCGTCAGATGT 
                   
               
               
                   
                 GTATAAGAGACAG 
                   
               
               
                   
               
               
                 Primer P7 for  
                 CAAGCAGAAGACGGCATACG 
                 67 
               
               
                 next-generation 
                 AGAT******** 
                   
               
               
                 sequencer 
                 GTCTCGTGGGCTCGGAGATG 
                   
               
               
                   
                 TGTATAAGAGACAG 
               
               
                   
               
            
           
         
       
     
     More specifically, the primers P7 used for a next-generation sequencer comprising 96 types of index sequences were designed (Table 4). When the primer P7 used for a next-generation sequencer shown in Table 4 was used, the primer P5 used for a next-generation sequencer composed of the sequence: AATGATACGGCGACCACCGA-GATCTACACCGCGCAGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG, was used. A DNA library prepared with the use of the primer P7 used for a next-generation sequencer shown in Table 4 is referred to as “DNA library 1.” 
                                 TABLE 4                       Number   SEQ               of   ID       Primer P7 for next-generation sequencer   Index   reads   NO                                                CAAGCAGAAGACGGCATACGAGATGTGATACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGTATCAC   2698   68               CAAGCAGAAGACGGCATACGAGATGAGCGCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACGCGCTC   11021   69               CAAGCAGAAGACGGCATACGAGATGCATCTCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGAGATGC   102619   70               CAAGCAGAAGACGGCATACGAGATTCGTACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACGTACGA   36922   71               CAAGCAGAAGACGGCATACGAGATTATCGTCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGACGATA   74371   72               CAAGCAGAAGACGGCATACGAGATTGCACAGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TCTGTGCA   77759   73               CAAGCAGAAGACGGCATACGAGATCGTATGACGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GTCATACG   65129   74               CAAGCAGAAGACGGCATACGAGATTCGATCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GAGATCGA   77012   75               CAAGCAGAAGACGGCATACGAGATATATGACAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TGTCATAT   83490   76               CAAGCAGAAGACGGCATACGAGATGCTATCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACGATAGC   21633   77               CAAGCAGAAGACGGCATACGAGATCTGTGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACACACAG   18188   78               CAAGCAGAAGACGGCATACGAGATTATACTGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GCAGTATA   59936   79               CAAGCAGAAGACGGCATACGAGATACTGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACGACAGT   46354   80               CAAGCAGAAGACGGCATACGAGATGAGCTATAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TATAGCTC   103670   81               CAAGCAGAAGACGGCATACGAGATTGTGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACGACACA   22327   82               CAAGCAGAAGACGGCATACGAGATGTGACTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ATAGTCAC   29747   83               CAAGCAGAAGACGGCATACGAGATTATACAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACTGTATA   38852   84               CAAGCAGAAGACGGCATACGAGATATGAGCGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TCGCTCAT   68292   85               CAAGCAGAAGACGGCATACGAGATAGATCAGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TCTGATCT   102628   86               CAAGCAGAAGACGGCATACGAGATAGTCTGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TGCAGACT   79949   87               CAAGCAGAAGACGGCATACGAGATCGCTGTGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TCACAGCG   96884   88               CAAGCAGAAGACGGCATACGAGATGTCTATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACATAGAC   29836   89               CAAGCAGAAGACGGCATACGAGATCTGATGTGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CACATCAG   92829   90               CAAGCAGAAGACGGCATACGAGATCGCACTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ATAGTGCG   16800   91               CAAGCAGAAGACGGCATACGAGATAGTGTGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGCACACT   95836   92               CAAGCAGAAGACGGCATACGAGATCGTATCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GCGATACG   55672   93               CAAGCAGAAGACGGCATACGAGATGCACTCTAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TAGAGTGC   84433   94               CAAGCAGAAGACGGCATACGAGATTACGACACGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GTGTCGTA   69554   95               CAAGCAGAAGACGGCATACGAGATTCTGCTCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TGAGCAGA   78854   96               CAAGCAGAAGACGGCATACGAGATTCGTGACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGTCACGA   902   97               CAAGCAGAAGACGGCATACGAGATTGTATCTAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TAGATACA   106290   98               CAAGCAGAAGACGGCATACGAGATGTGCGTACGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GTACGCAC   84862   99               CAAGCAGAAGACGGCATACGAGATCACTACTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GAGTAGTG   72290   100               CAAGCAGAAGACGGCATACGAGATTGAGCGTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GACGCTCA   59639   101               CAAGCAGAAGACGGCATACGAGATTGACGTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGACGTCA   599   102               CAAGCAGAAGACGGCATACGAGATACAGTGAGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CTCACTGT   113481   103               CAAGCAGAAGACGGCATACGAGATGACTCTCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TGAGAGTC   107121   104               CAAGCAGAAGACGGCATACGAGATAGCGCGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGCGCGCT   3743   105               CAAGCAGAAGACGGCATACGAGATCTGTAGAGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CTCTACAG   94729   106               CAAGGAGAAGACGGCATACGAGATATGCGATAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TATCGCAT   88451   107               CAAGGAGAAGACGGCATACGAGATGAGACATGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CATGTCTC   103682   108               CAAGGAGAAGACGGCATACGAGATGTCATGTGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CACATGAC   108326   109               CAAGCAGAAGACGGCATACGAGATTCATGATAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TATCATGA   100548   110               CAAGGAGAAGACGGCATACGAGATGTCATCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GAGATGAC   95911   111               CAAGCAGAAGACGGCATACGAGATAGTGTCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GCGACACT   63077   112               CAAGCAGAAGACGGCATACGAGATGCTGACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACGTCAGC   36541   113               CAAGCAGAAGACGGCATACGAGATGATCAGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGCTGATC   1524   114               CAAGCAGAAGACGGCATACGAGATTATCTCTAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TAGAGATA   97320   115               CAAGGAGAAGACGGCATACGAGATGCAGAGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGCTCTGC   76234   116               CAAGCAGAAGACGGCATACGAGATTGCTAGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGCTAGCA   69861   117               CAAGCAGAAGACGGCATACGAGATCGTATCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GAGATACG   88530   118               CAAGCAGAAGACGGCATACGAGATCTGATATAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TATATCAG   43949   119               CAAGCAGAAGACGGCATACGAGATTAGTGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GCGCACTA   50049   120               CAAGCAGAAGACGGCATACGAGATCTAGTGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TGCACTAG   88820   121               CAAGCAGAAGACGGCATACGAGATGATGTCACGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GTGACATC   64527   122               CAAGCAGAAGACGGCATACGAGATATAGAGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGCTCTAT   51356   123               CAAGGAGAAGACGGCATACGAGATAGACATATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ATATGTCT   102442   124               CAAGGAGAAGACGGCATACGAGATCGATCATGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CATGATCG   74522   125               CAAGCAGAAGACGGCATACGAGATACATAGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGCTATGT   87460   126               CAAGCAGAAGACGGCATACGAGATATCGACACGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GTGTCGAT   70762   127               CAAGCAGAAGACGGCATACGAGATTACACACGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGTGTGTA   78672   128               CAAGCAGAAGACGGCATACGAGATTACGCATGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CATGCGTA   87658   129               CAAGCAGAAGACGGCATACGAGATCGTGAGTGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CACTCACG   16880   130               CAAGGAGAAGACGGCATACGAGATGTCTGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ATGCAGAC   88654   131               CAAGCAGAAGACGGCATACGAGATGCATATGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TCATATGC   466   132               CAAGGAGAAGACGGCATACGAGATTGCTCTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGAGAGCA   79993   133               CAAGGAGAAGACGGCATACGAGATGACACATGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CATGTGTC   67887   134               CAAGGAGAAGACGGCATACGAGATCTGAGCAGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CTGCTCAG   69388   135               CAAGGAGAAGACGGCATACGAGATGACATGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGCATGTC   85651   136               CAAGCAGAAGACGGGATAGGAGATTCTGACGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TCGTCAGA   18576   137               CAAGCAGAAGACGGCATACGAGATTACAGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ACACTGTA   50570   138               CAAGCAGAAGACGGCATACGAGATGATCGCAGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CTGCGATC   103807   139               CAAGGAGAAGACGGCATACGAGATACATGACGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGTCATGT   88366   140               CAAGGAGAAGACGGCATACGAGATCTAGATGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TCATCTAG   98908   141               CAAGGAGAAGACGGCATACGAGATGCGTCTAGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CTAGACGC   53858   142               CAAGCAGAAGACGGCATACGAGATACTCGTGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GCACGAGT   95266   143               CAAGGAGAAGACGGCATACGAGATICACGCTGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CAGCGTGA   577   144               CAAGCAGAAGACGGCATACGAGATCTAGATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGATCTAG   64106   145               CAAGGAGAAGACGGCATACGAGATCGATAGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TGCTATCG   900   146               CAAGCAGAAGACGGCATACGAGATATCGTACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   AGTACGAT   103699   147               CAAGGAGAAGACGGCATACGAGATTCATGTACGTGTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GTACATGA   107163   148               CAAGCAGAAGACGGCATACGAGATTAGTGACGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGTCACTA   107188   149               CAAGCAGAAGACGGCATACGAGATCACGATAGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CTATCGTG   78472   150               CAAGCAGAAGACGGCATACGAGATACACACTGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CAGTGTGT   63028   151               CAAGCAGAAGACGGCATACGAGATCGTCTAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GCTAGACG   67417   152               CAAGCAGAAGACGGCATACGAGATTAGCTAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GCTAGCTA   62143   153               CAAGCAGAAGACGGCATACGAGATTCGACGTCGTCTCGTGGGCTCGGAGATTIGTATAAGAGACAG   GACGTCGA   104854   154               CAAGCAGAAGACGGCATACGAGATAGCATCACGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GTGATGCT   64287   155               CAAGCAGAAGACGGCATACGAGATCGAGACGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GCGTCTCG   64524   156               CAAGCAGAAGACGGCATACGAGATCGCGAGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TGCTCGCG   100457   157               CAAGCAGAAGACGGCATACGAGATTAGTCGTAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   TACGACTA   25668   158               CAAGCAGAAGACGGCATACGAGATCGTAGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ATACTACG   91426   159               CAAGCAGAAGACGGCATACGAGATTCACGTACGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GTACGTGA   75359   160               CAAGGAGAAGACGGCATACGAGATGCATGGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   CGCGATGC   103505   161               CAAGGAGAAGACGGCATACGAGATATCATGTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   GACATGAT   22828   162               CAAGCAGAAGACGGCATACGAGATCTATGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG   ATGCATAG   85282   163                    
Separately, the primers P5 used for a next-generation sequencer comprising 98 types of index sequences were designed (Table 5). When the primer P5 used for a next-generation sequencer shown in Table 5 was used, the primer P7 used for a next-generation sequencer composed of the sequence: CAAGCAGAAGACGGCATACGA-GATTCGTCAGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG, was used. A DNA library prepared with the use of the primer P5 used for a next-generation sequencer shown in Table 5 is referred to as “DNA library 2.”
 
                                 TABLE 5                       Number   SEQ               of   ID       Primer P5 for next-generation sequencer   Index   reads   NO                  AATGATACGGCGACCACCGAGATCTACACCTGCTCGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CTGCTCGT   184097   164               AATGATACGGCGACCACCGAGATCTACACTGCGACGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGCGACGA   148883   165               AATGATACGGCGACCACCGAGATCTACACCACATGCTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CACATGCT   202711   166               AATGATACGGCGACCACCGAGATCTACACATACTCATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ATACTCAT   206240   167               AATGATACGGCGACCACCGAGATCTACACGATGCACGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GATGCACG   184211   168               AATGATACGGCGACCACCGAGATCTACACGTAGTGCTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GTAGTGCT   217994   169               AATGATACGGCGACCACCGAGATCTACACATGATAGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ATGATAGT   208012   170               AATGATACGGCGACCACCGAGATCTACACCTCGCTAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CTCGCTAG   161874   171               AATGATACGGCGACCACCGAGATCTACACGCTGAGAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GCTGAGAG   194463   172               AATGATACGGCGACCACCGAGATCTACACAGCACGAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGCACGAG   216430   173               AATGATACGGCGACCACCGAGATCTACACTGTCAGAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGTCAGAG   117194   174               AATGATACGGCGACCACCGAGATCTACACGCGCAGTATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GCGCAGTA   217454   175               AATGATACGGCGACCACCGAGATCTACACCATCAGCGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CATCAGCG   204045   176               AATGATACGGCGACCACCGAGATCTACACTACGAGCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TACGAGCA   207318   177               AATGATACGGCGACCACCGAGATCTACACAGCAGACTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGCAGACT   199285   178               AATGATACGGCGACCACCGAGATCTACACCAGTACATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CAGTACAT   213283   179               AATGATACGGCGACCACCGAGATCTACACGAGTATGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GAGTATGA   194615   180               AATGATACGGCGACCACCGAGATCTACACTATCACATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TATCACAT   234075   181               AATGATACGGCGACCACCGAGATCTACACCACAGTCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CACAGTCA   146176   182               AATGATACGGCGACCACCGAGATCTACACTGCAGCTATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGCAGCTA   219031   183               AATGATACGGCGACCACCGAGATCTACACGCGAGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GCGAGCAG   199643   184               AATGATACGGCGACCACCGAGATCTACACGACAGCGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GACAGCGT   166387   185               AATGATACGGCGACCACCGAGATCTACACAGCTCGAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGCTCGAG   182808   186               AATGATACGGCGACCACCGAGATCTACACTAGATCATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TAGATCAT   208157   187               AATGATACGGCGACCACCGAGATCTACACCGCAGTGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGCAGTGA   211346   188               AATGATACGGCGACCACCGAGATCTACACCGTACTGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGTACTGA   194225   189               AATGATACGGCGACCACCGAGATCTACACTCAGATGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TCAGATGT   165740   190               AATGATACGGCGACCACCGAGATCTACACCTCTCTGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CTCTCTGA   157184   191               AATGATACGGCGACCACCGAGATCTACACTCAGCATATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TCAGCATA   226142   192               AATGATACGGCGACCACCGAGATCTACACCATACAGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CATACAGA   195115   193               AATGATACGGCGACCACCGAGATCTACACCGAGACGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGAGACGA   186104   194               AATGATACGGCGACCACCGAGATCTACACCTCGACAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CTCGACAG   175716   195               AATGATACGGCGACCACCGAGATCTACACGTAGATGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GTAGATGA   198272   196               AATGATACGGCGACCACCGAGATCTACACCATCTCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CATCTCAG   162985   197               AATGATACGGCGACCACCGAGATCTACACTGAGCTCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGAGCTCA   190928   198               AATGATACGGCGACCACCGAGATCTACACTAGAGCGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TAGAGCGT   213271   199               AATGATACGGCGACCACCGAGATCTACACAGATAGCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGATAGCA   207685   200               AATGATACGGCGACCACCGAGATCTACACAGACTGAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGACTGAG   192521   201               AATGATACGGCGACCACCGAGATCTACACGCTACATATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GCTACATA   221694   202               AATGATACGGCGACCACCGAGATCTACACATAGCTATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ATAGCTAT   241220   203               AATGATACGGCGACCACCGAGATCTACACATCGAGTATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ATCGAGTA   155902   204               AATGATACGGCGACCACCGAGATCTACACGATGTGATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GATGTGAT   224048   205               AATGATACGGCGACCACCGAGATCTACACGATCAGTATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GATCAGTA   191406   206               AATGATACGGCGACCACCGAGATCTACACTATGTACTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TATGTACT   230743   207               AATGATACGGCGACCACCGAGATCTACACTCACTGCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TCACTGCA   226410   208               AATGATACGGCGACCACCGAGATCTACACACACATATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ACACATAT   216612   209               AATGATACGGCGACCACCGAGATCTACACCTCTCTAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CTCTCTAG   173746   210               AATGATACGGCGACCACCGAGATCTACACGTGAGTGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GTGAGTGT   186292   211               AATGATACGGCGACCACCGAGATCTACACCATCGATGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CATCGATG   193175   212               AATGATACGGCGACCACCGAGATCTACACGATGCACTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GATGCACT   219388   213               AATGATACGGCGACCACCGAGATCTACACCGTACTCGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGTACTCG   160838   214               AATGATACGGCGACCACCGAGATCTACACTGAGTGCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGAGTGCA   225841   215               AATGATACGGCGACCACCGAGATCTACACCGCGATGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGCGATGA   186952   216               AATGATACGGCGACCACCGAGATCTACACTGATCGCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGATCGCA   204285   217               AATGATACGGCGACCACCGAGATCTACACCGCGACTATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGCGACTA   193193   218               AATGATACGGCGACCACCGAGATCTACACATACGCATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ATACGCAT   214235   219               AATGATACGGCGACCACCGAGATCTACACCGAGCGCTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGAGCGCT   216910   220               AATGATACGGCGACCACCGAGATCTACACACTACGATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ACTACGAT   213769   221               AATGATACGGCGACCACCGAGATCTACACTGCAGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGCAGCAG   202449   222               AATGATACGGCGACCACCGAGATCTACACAGTACTCGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGTACTCG   169853   223               AATGATACGGCGACCACCGAGATCTACACCTGAGTGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CTGAGTGT   185566   224               AATGATACGGCGACCACCGAGATCTACACGTGAGTGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GTGAGTGA   189950   225               AATGATACGGCGACCACCGAGATCTACACTGTCGTCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGTCGTCA   147602   226               AATGATACGGCGACCACCGAGATCTACACCACGAGCTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CACGAGCT   185881   227               AATGATACGGCGACCACCGAGATCTACACGAGACTCTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GAGACTCT   166991   228               AATGATACGGCGACCACCGAGATCTACACCATGTCACTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CATGTCAC   169567   229               AATGATACGGCGACCACCGAGATCTACACCGTGTACGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGTGTACG   203769   230               AATGATACGGCGACCACCGAGATCTACACTCGTAGATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TCGTAGAT   174792   231               AATGATACGGCGACCACCGAGATCTACACAGCTGACATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGCTGACA   171467   232               AATGATACGGCGACCACCGAGATCTACACCGTCATCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGTCATCA   157255   233               AATGATACGGCGACCACCGAGATCTACACTACTCACGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TACTCACG   207149   234               AATGATACGGCGACCACCGAGATCTACACCAGTAGCGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CAGTAGCG   210288   235               AATGATACGGCGACCACCGAGATCTACACCATGTAGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CATGTAGT   213173   236               AATGATACGGCGACCACCGAGATCTACACTAGAGACGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TAGAGACG   180905   237               AATGATACGGCGACCACCGAGATCTACACCGTCTCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGTCTCAG   151938   238               AATGATACGGCGACCACCGAGATCTACACTACATGCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TACATGCA   210596   239               AATGATACGGCGACCACCGAGATCTACACTACTAGCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TACTAGCA   212182   240               AATGATACGGCGACCACCGAGATCTACACATGACAGATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ATGACAGA   186220   241               AATGATACGGCGACCACCGAGATCTACACACAGCGTATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ACAGCGTA   182287   242               AATGATACGGCGACCACCGAGATCTACACCATGCACTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CATGCACT   235606   243               AATGATACGGCGACCACCGAGATCTACACTCGAGCGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TCGAGCGT   206819   244               AATGATACGGCGACCACCGAGATCTACACCGCACGAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGCACGAG   182693   245               AATGATACGGCGACCACCGAGATCTACACCACAGTATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CACAGTAT   195608   246               AATGATACGGCGACCACCGAGATCTACACAGTCATCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGTCATCA   173057   247               AATGATACGGCGACCACCGAGATCTACACTGACTATATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGACTATA   208986   248               AATGATACGGCGACCACCGAGATCTACACTCGCATATTCGTCGGGAGCGTCAGATGTGTATAAGAGACAG   TCGCATAT   202226   249               AATGATACGGCGACCACCGAGATCTACACATCGATGTTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ATCGATGT   170032   250               AATGATACGGCGACCACCGAGATCTACACCGACAGCGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGACAGCG   204799   251               AATGATACGGCGACCACCGAGATCTACACATCGTGTATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   ATCGTGTA   187161   252               AATGATACGGCGACCACCGAGATCTACACGCATGTAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GCATGTAG   190133   253               AATGATACGGCGACCACCGAGATCTACACAGAGATCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   AGAGATCA   136140   254               AATGATACGGCGACCACCGAGATCTACACCGTCAGTATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   CGTCAGTA   141604   255               AATGATACGGCGACCACCGAGATCTACACGCGTAGATTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GCGTAGAT   172444   256               AATGATACGGCGACCACCGAGATCTACACGTACTACATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GTACTACA   204499   257               AATGATACGGCGACCACCGAGATCTACACGCGAGACATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GCGAGACA   181860   258               AATGATACGGCGACCACCGAGATCTACACGATAGACGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   GATAGACG   211211   259               AATGATACGGCGACCACCGAGATCTACACTATACTAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TATACTAG   230695   260               AATGATACGGCGACCACCGAGATCTACACTGCTCGCATCGTCGGCAGCGTCAGATGTGTATAAGAGACAG   TGCTCGCA   222050   261                    
The nucleic acid amplification reactions to prepare DNA library 1 and DNA library 2 were carried out under thermal cycling conditions comprising 95 degrees C. for 2 minutes, 25 cycles of 98 degrees C. for 15 seconds, 55 degrees C. for 15 seconds, and 72 degrees C. for 20 seconds, and 72 degrees C. for 1 minute, followed by storage at 4 degrees C. The DNA libraries prepared via the nucleic acid amplification reactions were purified with the use of the MinElute PCR Purification Kit (QIAGEN).
 
     3.4 Analysis Using Next-Generation Sequencer 
     With the use of the MiSeq Reagent Kit V2 500 Cycle (Illumina), the DNA libraries 1 and 2 prepared in 3.3 above were analyzed via 100 base paired-end sequencing. The number of reads concerning the DNA libraries 1 and 2 obtained as a result of analysis are shown in Table 4 and Table 5. 
     3.5 Analysis of Index Sequence and Amount of Data 
     On the basis of the number of reads for each primer used for a next-generation sequencer obtained as a result of analysis conducted in 3.4 above, the correlation between 8 nucleotides of the index sequence contained in the primer used for a next-generation sequencer and the amount of data was analyzed by the GLMNET LASSO method, and an estimation formula for calculating the number of reads based on the analyzed correlation and the types of nucleotides included in the index sequence was prepared. The correlational efficient between the putative number of reads calculated with the estimation formula and the measured number of reads was determined. 
     4. Results 
     4.1 Distribution of the Number of Reads of Each Primer Used for a Next-Generation Sequencer 
     In order to evaluate the influence of the primer used for a next-generation sequencer on the amount of data caused by different index sequences, the distribution of the number of reads of each of the primers P7 and P5 used for a next-generation sequencer comprising different index sequences was inspected. In the case of DNA library 1 in which the primer P7 used for a next-generation sequencer has a different index sequence, there were 9 types of index sequences (94%) exhibiting the number of reads of 15,000 or less, relative to the average number of the reads of the whole (i.e., 66,961.7), and the average was 2,492.2, which was 3.7% of the average of the whole ( FIG. 2 ). Also, there were 22 types of index sequences (220%) exhibiting the number of reads of 40,000 or less, and the average was 16,237.6, which was 24.3% of the average of the whole. In the case of DNA library 2 in which the primer P5 used for a next-generation sequencer has a different index sequence, the average number of the reads of the whole was 191,523.1. That is, all the index sequences exhibited the number of reads of 100,000 or less ( FIG. 3 ). 
     4.2. Correlation Between Index Sequence and Number of Reads 
     DNA library 1 and DNA library 2 were separately analyzed with the use of the next-generation sequencer, the correlation between the index sequence and the number of reads was analyzed based on the results of analysis by the GLMNET LASSO method, and the estimation formula that calculates the correlation between the nucleotide type and the number of reads of the index sequence was prepared. Specifically, an estimation formula designating the number of reads as a purpose variable and a nucleotide type of the index sequence as an explanatory variable was prepared.  FIG. 4  shows the correlation between the putative number of reads calculated with the use of the estimation formula prepared for DNA library 1 and the measured number of reads. As shown in  FIG. 4 , the correlational coefficient (r) between the putative number of reads determined with the estimation formula and the measured number of reads based on types of nucleotides constituting the index sequence was 0.94069 (r: 0.94069). As shown in  FIG. 4 , plots of the putative number of reads and the measured number of reads were classified into: Group 1 with a very small number of reads; Group 3 with a large number of reads; and Group 2 with a number of reads therebetween. The maximal putative number of reads determined based on the index sequence of the primer P7 used for a next-generation sequencer of Group 1 was 20,051.8. The putative number of reads determined based on the index sequence of the primer P7 used for a next-generation sequencer of Group 3 was 50,000 or greater. 
     Separately, an estimation formula concerning DNA library 2 was also calculated, the correlational coefficient (r) between the putative number of reads determined based on the types of nucleotides constituting index sequence and the measured number of reads was determined, and the correlational coefficient (r) was 0.57295 ( FIG. 5 ). Specifically, no correlation was observed between the types of nucleotides constituting the index sequence and the number of reads concerning the primer P5 used for a next-generation sequencer. 
     4.3 Design of the Index Sequence of the Primer P7 Used for a Next-Generation Sequencer 
     In the case of DNA library 1 in which the primer P7 used for a next-generation sequencer has a different index sequence, the correlational coefficient between the number of reads and the putative number of reads calculated by the GLMNET LASSO method was 0.9 or higher. On the basis thereof, the estimation formula prepared for DNA library 1 by the GLMNET LASSO method was evaluated to be capable of calculating the putative number of reads with high accuracy based on types of nucleotides constituting the index sequence. Specifically, the estimation formula prepared by the GLMNET LASSO method contains items and segments including a coefficient calculated for each nucleotide at a given position in an index sequence as shown below. 
     Putative number: −65033.1×(A1)+1326.4×(C1)−16997×(G1)+10936.3×(A2)−12399.2×(G2)+11712.9×(T2)+12112.2×(A3)−623.5×(G3)+5964.4×(T3)+6884.5×(A4)−5664.4×(C4)−6049.9×(G4)+9257×(A5)−6210.8×(G5)−644×(C6)+3.2×(T6)−3575.9×(A7)+1013.1×(G7)−8607.7×(G8)+6490.3×(T8)+81720.7 
     In the estimation formula, (A1) is a parameter to which “1” is assigned when the first nucleotide in the 5′ to 3′ direction in the index sequence is adenine and “0” is assigned under other conditions. Other notations also refer to positions in the 5′ to 3′ direction in the index sequence and nucleotide types, which are parameters to which “1” or “0” is assigned. 
     With the use of the estimation formula prepared in the manner described above, the primer P7 used for a next-generation sequencer exhibiting a putative number of 20,052 or higher (which is deduced to be Group 2 or 3) and the primer P7 used for a next-generation sequencer exhibiting a putative number of 50,000 or higher (which is deduced to be Group 3) were selected (Tables 6 and 7). In the nucleotide sequences shown in Tables 6 and 7, “N” represents an arbitrary nucleotide selected from among adenine, cytosine, guanine, and thymine. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Table 6: Primer P7 for next-generation sequencer and index thereof 
               
            
           
           
               
               
               
            
               
                   
                   
                 SEQ 
               
               
                   
                   
                 ID 
               
               
                 Primer P7 for next-generation sequencer 
                 Index 
                 NO 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAAGNNG 
                 262 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTCGTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAACGAGG 
                 263 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCCGTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAACGGGG 
                 264 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCACGTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAACGTGG 
                 265 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAANANNN 
                 266 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNGNTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAANCNNN 
                 267 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCNTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAANGNNA 
                 268 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCNTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAANGNNC 
                 269 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAANGNNT 
                 270 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNANTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAANTNNN 
                 271 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCATTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAATGNNG 
                 272 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAANNG 
                 273 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACACNNA 
                 274 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACACNNC 
                 275 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACACNNG 
                 276 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAGNGG 
                 277 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAGNNA 
                 278 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAGNNC 
                 279 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAGNNT 
                 280 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACATNNA 
                 281 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACATNNC 
                 282 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACATNNG 
                 283 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCANCG 
                 284 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCANGG 
                 285 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCANTG 
                 286 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCCNCA 
                 287 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCCNCC 
                 288 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCCNGA 
                 289 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCCNGC 
                 290 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCCNTA 
                 291 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCCNTC 
                 292 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCGNCT 
                 293 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCGNGT 
                 294 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCGNTT 
                 295 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCTNCA 
                 296 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCTNCC 
                 297 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCTNGA 
                 298 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCTNGC 
                 299 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCTNTA 
                 300 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAGGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACCTNTC 
                 301 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGANCG 
                 302 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGANGG 
                 303 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGANTG 
                 304 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGCNCA 
                 305 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGCNCC 
                 306 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGCNGA 
                 307 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGCNGC 
                 308 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGCNTA 
                 309 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGCNTC 
                 310 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGGNCT 
                 311 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGGNGT 
                 312 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCCGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGGNTT 
                 313 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNACGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGTNCA 
                 314 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNACGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGTNCC 
                 315 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNACGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGTNGA 
                 316 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNACGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGTNGC 
                 317 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANACGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGTNTA 
                 318 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANACGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACGTNTC 
                 319 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACNANNA 
                 320 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACNANNC 
                 321 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACNANNT 
                 322 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACNCNNT 
                 323 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACNTNNT 
                 324 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTANNG 
                 325 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTCNNA 
                 326 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTCNNC 
                 327 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTGNCA 
                 328 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTGNCC 
                 329 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTGNGA 
                 330 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTGNGC 
                 331 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTGNNT 
                 332 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTGNTA 
                 333 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTGNTC 
                 334 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNAAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTTNNA 
                 335 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNAAGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACTTNNC 
                 336 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGAANNG 
                 337 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGACNNA 
                 338 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGACNNC 
                 339 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGACNNG 
                 340 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTCTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGAGAGG 
                 341 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCCTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGAGGGG 
                 342 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGAGNNA 
                 343 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGAGNNC 
                 344 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGAGNNT 
                 345 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCACTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGAGTGG 
                 346 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGATNNA 
                 347 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGATNNC 
                 348 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGATNNG 
                 349 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCANCG 
                 350 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCANGG 
                 351 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCANTG 
                 352 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCCNCA 
                 353 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCCNCC 
                 354 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCCNGA 
                 355 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCCNGC 
                 356 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCCNTA 
                 357 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCCNTC 
                 358 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCGNCT 
                 359 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCGNGT 
                 360 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCGNTT 
                 361 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCTNCA 
                 362 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCTNCC 
                 363 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCTNGA 
                 364 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCTNGC 
                 365 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCTNTA 
                 366 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAGCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGCTNTC 
                 367 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGANCG 
                 368 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGANGG 
                 369 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGANTG 
                 370 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGCNCA 
                 371 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGCNCC 
                 372 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGCNGA 
                 373 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGCNGC 
                 374 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGCNTA 
                 375 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGCNTC 
                 376 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGGNCT 
                 377 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGGNGT 
                 378 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCCCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGGNTT 
                 379 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNACCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGTNCA 
                 380 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNACCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGTNCC 
                 381 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNACCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGTNGA 
                 382 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNACCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGTNGC 
                 383 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANACCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGTNTA 
                 384 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANACCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGGTNTC 
                 385 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGNANNA 
                 386 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGNANNC 
                 387 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGNANNT 
                 388 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGNCNNT 
                 389 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGNTNNT 
                 390 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTANNG 
                 391 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTCNNA 
                 392 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTCNNC 
                 393 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTGNCA 
                 394 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTGNCC 
                 395 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTGNGA 
                 396 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTGNGC 
                 397 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTGNNT 
                 398 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTGNTA 
                 399 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTGNTC 
                 400 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNAACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTTNNA 
                 401 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNAACTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGTTNNC 
                 402 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATACNNG 
                 403 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATAGNNA 
                 404 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATAGNNC 
                 405 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATAGNNG 
                 406 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATATNNG 
                 407 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTGGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCCAGG 
                 408 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCGGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCCGGG 
                 409 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCAGGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCCTGG 
                 410 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCGNCA 
                 411 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCGNCC 
                 412 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCGNGA 
                 413 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCGNGC 
                 414 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCGNTA 
                 415 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCGNTC 
                 416 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTAGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCTAGG 
                 417 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCAGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCTGGG 
                 418 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCAAGATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATCTTGG 
                 419 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCCATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATGGNCA 
                 420 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCCATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATGGNCC 
                 421 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCCATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATGGNGA 
                 422 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCCATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATGGNGC 
                 423 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCCATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATGGNTA 
                 424 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCCATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATGGNTC 
                 425 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATNANNN 
                 426 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGNATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATNCNNA 
                 427 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGNATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATNCNNC 
                 428 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATNCNNT 
                 429 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATNGNNT 
                 430 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNANATTGTCTCGTGGGGTCGGAGATGTGTATAAGAGACAG 
                 AATNTNNA 
                 431 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNANATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATNTNNC 
                 432 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATNTNNT 
                 433 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGAATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATTCNNG 
                 434 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCAATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATTGNNA 
                 435 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCAATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATTGNNC 
                 436 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNAAATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATTTNNG 
                 437 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAANNG 
                 438 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAACNNA 
                 439 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAACNNC 
                 440 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAACNNG 
                 441 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNCTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAGNCG 
                 442 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAGNGG 
                 443 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAGNNA 
                 444 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAGNNC 
                 445 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAGNNT 
                 446 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANCTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAGNTG 
                 447 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAATNNA 
                 448 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAATNNC 
                 449 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAATNNG 
                 450 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTTTGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACAAAG 
                 451 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTCTGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACAGAG 
                 452 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACANCG 
                 453 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACANGG 
                 454 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACANTG 
                 455 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTATGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACATAG 
                 456 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACCNCA 
                 457 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACCNCC 
                 458 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACCNGA 
                 459 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACCNGC 
                 460 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACCNTA 
                 461 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACCNTC 
                 462 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACGNCT 
                 463 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACGNGT 
                 464 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACGNTT 
                 465 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACTNCA 
                 466 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACTNCC 
                 467 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACTNGA 
                 468 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACTNGC 
                 469 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACTNTA 
                 470 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAGTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACACTNTC 
                 471 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGANCG 
                 472 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGANGG 
                 473 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGANTG 
                 474 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGCNCA 
                 475 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGCNCC 
                 476 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGCNGA 
                 477 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGCNGC 
                 478 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGCNTA 
                 479 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGCNTC 
                 480 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGGNCT 
                 481 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGGNGT 
                 482 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCCTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGGNTT 
                 483 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNACTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGTNCA 
                 484 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNACTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGTNCC 
                 485 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNACTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGTNGA 
                 486 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNACTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGTNGC 
                 487 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANACTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGTNTA 
                 488 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANACTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAGTNTC 
                 489 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACANANNA 
                 490 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACANANNC 
                 491 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACANCNNT 
                 492 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACANTNNT 
                 493 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATANNG 
                 494 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCACG 
                 495 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCATG 
                 496 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCGCG 
                 497 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCGTG 
                 498 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCNGG 
                 499 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCNNA 
                 500 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCNNC 
                 501 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCTCG 
                 502 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAGATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATCTTG 
                 503 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATGNCA 
                 504 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATGNCC 
                 505 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATGNGA 
                 506 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATGNGC 
                 507 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATGNNT 
                 508 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATGNTA 
                 509 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATGNTC 
                 510 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTACG 
                 511 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTATG 
                 512 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTGCG 
                 513 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTGTG 
                 514 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTNGG 
                 515 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTNNA 
                 516 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTNNC 
                 517 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTTCG 
                 518 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAAATGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACATTTTG 
                 519 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTTTTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAAAAG 
                 520 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTCTTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAAGAG 
                 521 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAANCG 
                 522 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAANGG 
                 523 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAANNA 
                 524 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAANNC 
                 525 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAANTG 
                 526 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTATTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAATAG 
                 527 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCACNCA 
                 528 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCACNCC 
                 529 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCACNGA 
                 530 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCACNGC 
                 531 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCACNNT 
                 532 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCACNTA 
                 533 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCACNTC 
                 534 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATTCTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAGAAT 
                 535 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATCCTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAGGAT 
                 536 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAGNCT 
                 537 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAGNGT 
                 538 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAGNTT 
                 539 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATACTGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCAGTAT 
                 540 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNATGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCATNCA 
                 541 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNATGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCATNCC 
                 542 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNATGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCATNGA 
                 543 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNATGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCATNGC 
                 544 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNATGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCATNNT 
                 545 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANATGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCATNTA 
                 546 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANATGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCATNTC 
                 547 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGTTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCAACA 
                 548 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGTTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCAACC 
                 549 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTATTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCAATA 
                 550 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGATTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCAATC 
                 551 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGCTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCAGCA 
                 552 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGCTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCAGCC 
                 553 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTACTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCAGTA 
                 554 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGACTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCAGTC 
                 555 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCANGA 
                 556 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNTGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCANGC 
                 557 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGATGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCATCA 
                 558 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGATGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCATCC 
                 559 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTAATGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCATTA 
                 560 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGAATGGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCCATTC 
                 561 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNTCGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCGANGA 
                 562 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNTCGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCGANGC 
                 563 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTAGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCTANNA 
                 564 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTAGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCTANNC 
                 565 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGAGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCTCNCT 
                 566 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGAGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCTCNGT 
                 567 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGAGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCTCNTT 
                 568 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNAAGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCTTNCT 
                 569 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNAAGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCTTNGT 
                 570 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANAAGGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACCTTNTT 
                 571 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGAANCG 
                 572 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGAANGG 
                 573 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGAANNA 
                 574 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGAANNC 
                 575 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGAANTG 
                 576 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGACNCA 
                 577 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGACNCC 
                 578 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGACNGA 
                 579 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGACNGC 
                 580 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGACNNT 
                 581 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGACNTA 
                 582 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGACNTC 
                 583 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGAGNCT 
                 584 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGAGNGT 
                 585 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCTCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGAGNTT 
                 586 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNATCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGATNCA 
                 587 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNATCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGATNCC 
                 588 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNATCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGATNGA 
                 589 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNATCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGATNGC 
                 590 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNATCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGATNNT 
                 591 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANATCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGATNTA 
                 592 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANATCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGATNTC 
                 593 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCTTGCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGCAAGA 
                 594 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCTTGCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGCAAGC 
                 595 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCCTGCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGCAGGA 
                 596 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCCTGCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGCAGGC 
                 597 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCATGCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGCATGA 
                 598 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCATGCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGCATGC 
                 599 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCTTCCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGGAAGA 
                 600 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCTTCCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGGAAGC 
                 601 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCCTCCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGGAGGA 
                 602 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCCTCCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGGAGGC 
                 603 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCATCCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGGATGA 
                 604 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCATCCGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGGATGC 
                 605 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGTANNA 
                 606 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGTANNC 
                 607 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGTCNCT 
                 608 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGTCNGT 
                 609 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGTCNTT 
                 610 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNAACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGTTNCT 
                 611 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNAACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGTTNGT 
                 612 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANAACGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACGTTNTT 
                 613 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNNGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACNNANNT 
                 614 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTAANNG 
                 615 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNGTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTACNCG 
                 616 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTACNGG 
                 617 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTACNNA 
                 618 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTACNNC 
                 619 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTACNNT 
                 620 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANGTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTACNTG 
                 621 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTAGNCA 
                 622 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTAGNCC 
                 623 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTAGNGA 
                 624 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTAGNGC 
                 625 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTAGNNT 
                 626 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTAGNTA 
                 627 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCTAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTAGNTC 
                 628 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNATAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTATNCG 
                 629 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNATAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTATNGG 
                 630 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTATNNA 
                 631 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTATNNC 
                 632 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNATAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTATNNT 
                 633 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANATAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTATNTG 
                 634 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGGAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTCCNCT 
                 635 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGGAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTCCNGT 
                 636 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGGAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTCCNTT 
                 637 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNAGAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTCTNCT 
                 638 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNAGAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTCTNGT 
                 639 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANAGAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTCTNTT 
                 640 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGCAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTGCNCT 
                 641 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGCAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTGCNGT 
                 642 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGCAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTGCNTT 
                 643 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNACAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTGTNCT 
                 644 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNACAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTGTNGT 
                 645 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANACAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTGTNTT 
                 646 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTNANNA 
                 647 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTNANNC 
                 648 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTANCG 
                 649 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTANGG 
                 650 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTANTG 
                 651 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTCNCA 
                 652 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTCNCC 
                 653 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTCNGA 
                 654 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTCNGC 
                 655 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTCNNT 
                 656 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTCNTA 
                 657 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTCNTC 
                 658 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTGNCT 
                 659 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTGNGT 
                 660 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTGNTT 
                 661 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTTNCA 
                 662 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTTNCC 
                 663 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTTNGA 
                 664 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTTNGC 
                 665 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNAAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTTNNT 
                 666 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTTNTA 
                 667 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAAAGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACTTTNTC 
                 668 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTTTTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAAAAG 
                 669 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTCTTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAAGAG 
                 670 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAANCG 
                 671 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAANGG 
                 672 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAANNA 
                 673 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAANNC 
                 674 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAANTG 
                 675 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTATTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAATAG 
                 676 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAACNCA 
                 677 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAACNCC 
                 678 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAACNGA 
                 679 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAACNGC 
                 680 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAACNNT 
                 681 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAACNTA 
                 682 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAACNTC 
                 683 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAGNCT 
                 684 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAGNGT 
                 685 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATAGGAGATAANCTTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAAGNTT 
                 686 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNATTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAATNCA 
                 687 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNATTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAATNCC 
                 688 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNATTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAATNGA 
                 689 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNATTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAATNGC 
                 690 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNATTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAATNNT 
                 691 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANATTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAATNTA 
                 692 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANATTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAATNTC 
                 693 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNTGTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGACANGA 
                 694 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNTGTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGACANGC 
                 695 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCTTCTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAGAAGA 
                 696 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCTTCTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAGAAGC 
                 697 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCCTCTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAGAGGA 
                 698 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCCTCTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAGAGGC 
                 699 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCATCTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAGATGA 
                 700 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCATCTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGAGATGC 
                 701 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNTCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGANANNT 
                 702 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGATANNA 
                 703 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGATANNC 
                 704 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGATCNCT 
                 705 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGATCNGT 
                 706 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGATCNTT 
                 707 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNAATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGATTNCT 
                 708 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNAATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGATTNGT 
                 709 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANAATCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGATTNTT 
                 710 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGTTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAAACA 
                 711 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGTTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAAACC 
                 712 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTATTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAAATA 
                 713 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGATTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAAATC 
                 714 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGCTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAAGCA 
                 715 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGCTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAAGCC 
                 716 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTACTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAAGTA 
                 717 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGACTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAAGTC 
                 718 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAANGA 
                 719 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAANGC 
                 720 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAANNT 
                 721 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGATTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAATCA 
                 722 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGATTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAATCC 
                 723 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTAATTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAATTA 
                 724 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGAATTGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCAATTC 
                 725 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTAGCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGCTANGT 
                 726 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNTTCCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGGAANGA 
                 727 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNTTCCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGGAANGC 
                 728 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTTCCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGGAANNT 
                 729 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACTTACCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGGTAAGT 
                 730 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACCTACCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGGTAGGT 
                 731 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACATACCTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGGTATGT 
                 732 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTTACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTAANNA 
                 733 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTTACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTAANNC 
                 734 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTTACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTAANNT 
                 735 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGTACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTACNCT 
                 736 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGTACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTACNGT 
                 737 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGTACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTACNTT 
                 738 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATAGTACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTACTAT 
                 739 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNATACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTATNCT 
                 740 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNATACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTATNGT 
                 741 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANATACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTATNTT 
                 742 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATAATACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTATTAT 
                 743 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGTTGACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTCAACT 
                 744 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAATTGACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTCAATT 
                 745 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGCTGACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTCAGCT 
                 746 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAACTGACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTCAGTT 
                 747 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTGACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTCANGT 
                 748 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGATGACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTCATCT 
                 749 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAAATGACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTCATTT 
                 750 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTCACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTGANGT 
                 751 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCTTAACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTTAAGA 
                 752 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCTTAACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTTAAGC 
                 753 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCCTAACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTTAGGA 
                 754 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCCTAACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTTAGGC 
                 755 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTAACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTTANNT 
                 756 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCATAACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTTATGA 
                 757 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCATAACTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AGTTATGC 
                 758 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAAGNNG 
                 759 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATACGNGG 
                 760 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTCCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGGAGG 
                 761 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCCCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGGGGG 
                 762 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCACCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGGTGG 
                 763 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATANANNN 
                 764 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNGNTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATANCNNN 
                 765 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCNTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATANGNNA 
                 766 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCNTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATANGNNC 
                 767 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATANGNNT 
                 768 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNANTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATANTNNN 
                 769 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCATATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATATGNNG 
                 770 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAANNG 
                 771 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCACNNA 
                 772 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCACNNC 
                 773 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCACNNG 
                 774 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTCTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGACG 
                 775 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATCTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGATG 
                 776 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCCTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGGCG 
                 777 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACCTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGGTG 
                 778 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGNGG 
                 779 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGNNA 
                 780 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGNNC 
                 781 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGNNT 
                 782 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGACTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGTCG 
                 783 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAACTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAGTTG 
                 784 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCATNNA 
                 785 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCATNNC 
                 786 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCATNNG 
                 787 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCANCG 
                 788 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCANGG 
                 789 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCANTG 
                 790 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCCNCA 
                 791 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCCNCC 
                 792 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCCNGA 
                 793 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCCNGC 
                 794 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCCNTA 
                 795 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCCNTC 
                 796 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCGNCT 
                 797 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCGNGT 
                 798 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCGNTT 
                 799 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCTNCA 
                 800 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCTNCC 
                 801 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCTNGA 
                 802 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCTNGC 
                 803 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCTNTA 
                 804 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAGGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCCTNTC 
                 805 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGANCG 
                 806 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGANGG 
                 807 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGANTG 
                 808 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGCNCA 
                 809 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGCNCC 
                 810 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGCNGA 
                 811 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGCNGC 
                 812 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGCNTA 
                 813 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGCNTC 
                 814 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGGNCT 
                 815 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGGNGT 
                 816 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCCGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGGNTT 
                 817 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNACGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGTNCA 
                 818 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNACGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGTNCC 
                 819 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNACGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGTNGA 
                 820 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNACGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGTNGC 
                 821 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANACGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGTNTA 
                 822 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANACGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCGTNTC 
                 823 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCNANNA 
                 824 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCNANNC 
                 825 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCNANNT 
                 826 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCNCNNT 
                 827 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCNTNNT 
                 828 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTANNG 
                 829 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTCNGG 
                 830 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTCNNA 
                 831 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTCNNC 
                 832 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTGNCA 
                 833 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTGNCC 
                 834 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTGNGA 
                 835 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTGNGC 
                 836 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTGNNT 
                 837 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTGNTA 
                 838 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTGNTC 
                 839 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNAAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTTNGG 
                 840 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNAAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTTNNA 
                 841 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNAAGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCTTNNC 
                 842 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAANNG 
                 843 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGACNNA 
                 844 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGACNNC 
                 845 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGACNNG 
                 846 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAGNGG 
                 847 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAGNNA 
                 848 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAGNNC 
                 849 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAGNNT 
                 850 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGATNNA 
                 851 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGATNNC 
                 852 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGATNNG 
                 853 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCANCG 
                 854 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCANGG 
                 855 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCANTG 
                 856 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCCNCA 
                 857 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCCNCC 
                 858 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCCNGA 
                 859 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCCNGC 
                 860 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCCNTA 
                 861 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCCNTC 
                 862 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCGNCT 
                 863 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCGNGT 
                 864 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCGNTT 
                 865 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCTNCA 
                 866 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCTNCC 
                 867 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCTNGA 
                 868 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCTNGC 
                 869 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCTNTA 
                 870 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAGCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGCTNTC 
                 871 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGANCG 
                 872 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGANGG 
                 873 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGANTG 
                 874 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGCNCA 
                 875 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGCNCC 
                 876 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGCNGA 
                 877 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGCNGC 
                 878 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGCNTA 
                 879 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGCNTC 
                 880 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGGNCT 
                 881 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGGNGT 
                 882 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCCCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGGNTT 
                 883 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNACCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGTNCA 
                 884 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNACCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGTNCC 
                 885 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNACCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGTNGA 
                 886 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNACCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGTNGC 
                 887 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANACCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGTNTA 
                 888 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANACCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGGTNTC 
                 889 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGNANNA 
                 890 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGNANNC 
                 891 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGNANNT 
                 892 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGNCNNT 
                 893 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGNTNNT 
                 894 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTANNG 
                 895 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTGACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTCAGG 
                 896 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCGACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTCGGG 
                 897 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTCNNA 
                 898 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTCNNC 
                 899 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCAGACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTCTGG 
                 900 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTGNCA 
                 901 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTGNCC 
                 902 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTGNGA 
                 903 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTGNGC 
                 904 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTGNNT 
                 905 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTGNTA 
                 906 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTGNTC 
                 907 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTAACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTTAGG 
                 908 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCAACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTTGGG 
                 909 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNAACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTTNNA 
                 910 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNAACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTTNNC 
                 911 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCAAACATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGTTTGG 
                 912 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTACNNG 
                 913 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAGNNA 
                 914 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAGNNC 
                 915 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAGNNG 
                 916 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTATNNG 
                 917 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTGGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCCACG 
                 918 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATGGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCCATG 
                 919 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCGGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCCGCG 
                 920 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACGGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCCGTG 
                 921 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCCNGG 
                 922 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAGGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCCTCG 
                 923 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAGGAATUTCTCGTGGGCTCGGAGATUTGTATAAGAGACAG 
                 ATTCCTTG 
                 924 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCGNCA 
                 925 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCGNCC 
                 926 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCGNGA 
                 927 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCGNGC 
                 928 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCGNTA 
                 929 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCGNTC 
                 930 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTAGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCTACG 
                 931 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATAGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCTATG 
                 932 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCAGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCTGCG 
                 933 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACAGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCTGTG 
                 934 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNAGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCTNGG 
                 935 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAAGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCTTCG 
                 936 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAAGAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTCTTTG 
                 937 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGCAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTGCNGG 
                 938 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCCAATGTTTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTGGNCA 
                 939 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCCAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTGGNCC 
                 940 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCCAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTGGNGA 
                 941 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCCAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTGGNGC 
                 942 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCCAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTGGNTA 
                 943 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCCAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTGGNTC 
                 944 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNACAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTGTNGG 
                 945 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTNANNN 
                 946 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGNAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTNCNNA 
                 947 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGNAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTNCNNC 
                 948 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTNCNNT 
                 949 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTNGNNT 
                 950 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNANAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTNTNNA 
                 951 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNANAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTNTNNC 
                 952 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTNTNNT 
                 953 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTCNNG 
                 954 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTCAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTGAGG 
                 955 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCCAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTGGGG 
                 956 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTGNNA 
                 957 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTGNNC 
                 958 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCACAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTGTGG 
                 959 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNAAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTTNNG 
                 960 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNNGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CNNNNNNN 
                 961 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNNCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GNNNNNNN 
                 962 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNNAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TNNNNNNN 
                 963 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Table 7: Primer P7 for next-generation sequencer and index thereof 
               
            
           
           
               
               
               
            
               
                   
                   
                 SEQ 
               
               
                   
                   
                 ID 
               
               
                 Primer P7 for next-generation sequencer 
                 Index 
                 NO 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTTTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAAANNA 
                  964 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTTTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAAANNC 
                  965 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTTTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAAANNT 
                  966 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGTTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAACNCT 
                  967 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGTTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAACNGT 
                  968 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGTTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAACNTT 
                  969 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNATTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAATNCT 
                  970 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNATTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAATNGT 
                  971 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANATTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAATNTT 
                  972 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTGTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAACANGT 
                  973 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACTTCTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAGAAGT 
                  974 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACCTCTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAGAGGT 
                  975 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACATCTTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAAGATGT 
                  976 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCTTATTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAATAAGA 
                  977 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCTTATTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAATAAGC 
                  978 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCCTATTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAATAGGA 
                  979 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCCTATTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAATAGGC 
                  980 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTATTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAATANNT 
                  981 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCATATTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAATATGA 
                  982 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCATATTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAATATGC 
                  983 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGTTTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAAACT 
                  984 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAATTTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAAATT 
                  985 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGCTTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAAGCT 
                  986 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAACTTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAAGTT 
                  987 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAANGT 
                  988 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGATTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAATCT 
                  989 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAAATTGTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AACAATTT 
                  990 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTTCTTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AAGAANGT 
                  991 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNTTATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATAANGA 
                  992 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNTTATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATAANGC 
                  993 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTTATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATAANNT 
                  994 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACTTAATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATTAAGT 
                  995 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACCTAATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATTAGGT 
                  996 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACATAATTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 AATTATGT 
                  997 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNTTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAANCT 
                  998 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAANGT 
                  999 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANTTTGTGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ACAAANTT 
                 1000 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAAANNA 
                 1001 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAAANNC 
                 1002 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAAANNT 
                 1003 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATTGTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAACAAT 
                 1004 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATCGTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAACGAT 
                 1005 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAACNCT 
                 1006 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAACNGT 
                 1007 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAACNTT 
                 1008 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATAGTTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAACTAT 
                 1009 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATTATTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAATAAT 
                 1010 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATCATTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAATGAT 
                 1011 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNATTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAATNCT 
                 1012 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNATTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAATNGT 
                 1013 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANATTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAATNTT 
                 1014 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATATAATTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAATTAT 
                 1015 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGTTGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATACAACT 
                 1016 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAATTGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATACAATT 
                 1017 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGCTGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATACAGCT 
                 1018 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAACTGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATACAGTT 
                 1019 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATACANGT 
                 1020 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGATGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATACATCT 
                 1021 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAAATGTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATACATTT 
                 1022 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGTTCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGAACT 
                 1023 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAATTCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGAATT 
                 1024 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGCTCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGAGCT 
                 1025 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAACTCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGAGTT 
                 1026 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGANGT 
                 1027 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGATCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGATCT 
                 1028 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAAATCTATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATAGATTT 
                 1029 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNTATATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATATANGA 
                 1030 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNTATATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATATANGC 
                 1031 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTATATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATATANNT 
                 1032 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNTTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAANCT 
                 1033 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAANGT 
                 1034 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANTTGATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATCAANTT 
                 1035 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGTTTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAAACT 
                 1036 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAATTTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAAATT 
                 1037 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGCTTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAAGCT 
                 1038 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAACTTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAAGTT 
                 1039 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAANGT 
                 1040 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGATTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAATCT 
                 1041 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAAATTCATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATGAATTT 
                 1042 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGTTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAAACA 
                 1043 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGTTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAAACC 
                 1044 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTATTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAAATA 
                 1045 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGATTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAAATC 
                 1046 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGCTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAAGCA 
                 1047 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGCTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAAGCC 
                 1048 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTACTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAAGTA 
                 1049 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGACTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAAGTC 
                 1050 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAANGA 
                 1051 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAANGC 
                 1052 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAANNT 
                 1053 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGATTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAATCA 
                 1054 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGATTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAATCC 
                 1055 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTAATTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAATTA 
                 1056 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGAATTAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTAATTC 
                 1057 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGTTAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTAACT 
                 1058 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAATTAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTAATT 
                 1059 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGCTAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTAGCT 
                 1060 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAACTAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTAGTT 
                 1061 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNTAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTANGT 
                 1062 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGATAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTATCT 
                 1063 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAAATAAATGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 ATTTATTT 
                 1064 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNTGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CANNNNNN 
                 1065 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNGGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CCNNNNNN 
                 1066 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNTCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGANNNNN 
                 1067 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCAGNNG 
                 1068 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTCGGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCCGACG 
                 1069 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATCGGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCCGATG 
                 1070 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCCGGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCCGGCG 
                 1071 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACCGGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCCGGTG 
                 1072 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCGGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCCGNGG 
                 1073 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGACGGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCCGTCG 
                 1074 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAACGGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCCGTTG 
                 1075 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCCGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCGGNGG 
                 1076 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCNANNN 
                 1077 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNGNGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCNCNNN 
                 1078 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCNGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCNGNNA 
                 1079 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCNGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCNGNNC 
                 1080 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCNGNNT 
                 1081 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNANGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCNTNNN 
                 1082 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCAGCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGCTGNNG 
                 1083 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGAGNNG 
                 1084 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTCGCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGCGAGG 
                 1085 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCCGCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGCGGGG 
                 1086 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCACGCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGCGTGG 
                 1087 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTCCCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGGGAGG 
                 1088 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCCCCCGGTOTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGGGGGG 
                 1089 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCACCCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGGGTGG 
                 1090 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNCCGGTCTCGTOGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGNANNN 
                 1091 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNGNCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGNCNNN 
                 1092 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCNCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGNGNNA 
                 1093 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCNCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGNGNNC 
                 1094 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGNGNNT 
                 1095 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNANCCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGNTNNN 
                 1096 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCACCGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGGTGNNG 
                 1097 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNACGOTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CGTNNNNN 
                 1098 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNAGGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 CTNNNNNN 
                 1099 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNTTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAANNNNN 
                 1100 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNGTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GACNNNNN 
                 1101 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGAGNNG 
                 1102 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCGCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGCGNNG 
                 1103 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTTCCCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGGGAAG 
                 1104 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTCCCCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGGGGAG 
                 1105 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNCCCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGGGNCG 
                 1106 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCCCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGGGNGG 
                 1107 
               
               
                   
               
               
                 CAAGCAGAAGACOGCATACGAGATCANCCCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGGGNTG 
                 1108 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTACCCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGGGTAG 
                 1109 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGNANNN 
                 1110 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNGNCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGNCNNN 
                 1111 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCNCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGNGNNA 
                 1112 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCNCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGNGNNC 
                 1113 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGNGNNT 
                 1114 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNANCTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGNTNNN 
                 1115 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCACTCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GAGTGNNG 
                 1116 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNATCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GATNNNNN 
                 1117 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNTGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCANNNNN 
                 1118 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCACNNG 
                 1119 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCAGNNA 
                 1120 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCAGNNC 
                 1121 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCAGNNG 
                 1122 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCATNNG 
                 1123 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTGGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCCACG 
                 1124 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATGGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCCATG 
                 1125 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCGGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCCGCG 
                 1126 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACGGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCCGTG 
                 1127 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCCNGG 
                 1128 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAGGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCCTCG 
                 1129 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAGGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCCTTG 
                 1130 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCGNCA 
                 1131 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCGNCC 
                 1132 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCGNGA 
                 1133 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCGNGC 
                 1134 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCGNTA 
                 1135 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCGNTC 
                 1136 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTAGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCTACG 
                 1137 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATAGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCTATG 
                 1138 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCAGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCTGCG 
                 1139 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACAGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCTGTG 
                 1140 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNAGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCTNGG 
                 1141 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAAGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCTTCG 
                 1142 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAAGGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCCTTTG 
                 1143 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTGCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGCACG 
                 1144 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATGCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGCATG 
                 1145 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCGCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGCGCG 
                 1146 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACGCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGCGTG 
                 1147 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGCNGG 
                 1148 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAGCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGCTCG 
                 1149 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAGCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGCTTG 
                 1150 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGGNCA 
                 1151 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGGNCC 
                 1152 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGGNGA 
                 1153 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGGNGC 
                 1154 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGGNTA 
                 1155 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCCGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGGNTC 
                 1156 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTACGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGTACG 
                 1157 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATACGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGTATG 
                 1158 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCACGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGTGCG 
                 1159 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACACGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGTGTG 
                 1160 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNACGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGTNGG 
                 1161 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAACGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGTTCG 
                 1162 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAACGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCGTTTG 
                 1163 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCNANNN 
                 1164 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGNGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCNCNNA 
                 1165 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGNGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCNCNNC 
                 1166 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCNCNNT 
                 1167 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCNGNNT 
                 1168 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNANGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCNTNNA 
                 1169 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNANGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCNTNNC 
                 1170 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCNTNNT 
                 1171 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGAGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCTCNNG 
                 1172 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCAGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCTGNGG 
                 1173 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCAGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCTGNNA 
                 1174 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCAGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCTGNNC 
                 1175 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNAAGGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCCTTNNG 
                 1176 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGACNNG 
                 1177 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGAGNNA 
                 1178 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGAGNNC 
                 1179 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGAGNNG 
                 1180 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGATNNG 
                 1181 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGCCNGG 
                 1182 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGCGNCA 
                 1183 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGCGNCC 
                 1184 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGCGNGA 
                 1185 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGCGNGC 
                 1186 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGCGNTA 
                 1187 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGCGNTC 
                 1188 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNAGCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGCTNGG 
                 1189 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTGCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGCAGG 
                 1190 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCGCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGCGGG 
                 1191 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCAGCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGCTGG 
                 1192 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGGNCA 
                 1193 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGGNCC 
                 1194 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGGNGA 
                 1195 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGGNGC 
                 1196 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGGNTA 
                 1197 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCCCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGGNTC 
                 1198 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTACCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGTAGG 
                 1199 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCACCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGTGGG 
                 1200 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCAACCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGGTTGG 
                 1201 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGNANNN 
                 1202 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGNCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGNCNNA 
                 1203 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGNCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGNCNNC 
                 1204 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGNCNNT 
                 1205 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGNGNNT 
                 1206 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNANCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGNTNNA 
                 1207 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNANCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGNTNNC 
                 1208 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANCGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGNTNNT 
                 1209 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGACGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGTCNNG 
                 1210 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTCACGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGTGAGG 
                 1211 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCCACGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGTGGGG 
                 1212 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCACGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGTGNNA 
                 1213 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCACGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGTGNNC 
                 1214 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCACACGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGTGTGG 
                 1215 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNAACGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCGTTNNG 
                 1216 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTAGNNG 
                 1217 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTCGAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTCGACG 
                 1218 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATCGAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTCGATG 
                 1219 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCCGAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTCGGCG 
                 1220 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACCGAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTCGGTG 
                 1221 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCGAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTCGNGG 
                 1222 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGACGAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTCGTCG 
                 1223 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAACGAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTCGTTG 
                 1224 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCCAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTGGNGG 
                 1225 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTNANNN 
                 1226 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNGNAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTNCNNN 
                 1227 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCNAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTNGNNA 
                 1228 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCNAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTNGNNC 
                 1229 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTNGNNT 
                 1230 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNANAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTNTNNN 
                 1231 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCAAGCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GCTTGNNG 
                 1232 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAACNNG 
                 1233 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAAGNNA 
                 1234 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAAGNNC 
                 1235 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAAGNNG 
                 1236 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAATNNG 
                 1237 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTGGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACCACG 
                 1238 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATGGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACCATG 
                 1239 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCGGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACCGCG 
                 1240 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACGGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACCGTG 
                 1241 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACCNGG 
                 1242 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAGGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACCTCG 
                 1243 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAGGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACCTTG 
                 1244 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACGNCA 
                 1245 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACGNCC 
                 1246 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACGNGA 
                 1247 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACGNGC 
                 1248 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACGNTA 
                 1249 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACGNTC 
                 1250 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTAGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACTACG 
                 1251 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATAGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACTATG 
                 1252 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCAGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACTGCG 
                 1253 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACAGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACTGTG 
                 1254 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNAGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACTNGG 
                 1255 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAAGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACTTCG 
                 1256 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAAGTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGACTTTG 
                 1257 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGCTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAGCNGG 
                 1258 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCCTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAGGNCA 
                 1259 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCCTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAGGNCC 
                 1260 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCCTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAGGNGA 
                 1261 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCCTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAGGNGC 
                 1262 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCCTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAGGNTA 
                 1263 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCCTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAGGNTC 
                 1264 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNACTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGAGTNGG 
                 1265 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGANANNN 
                 1266 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGNTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGANCNNA 
                 1267 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGNTCCGTCTCGTGGGCTCGGAGATTTGTATAAGAGACAG 
                 GGANCNNC 
                 1268 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGANCNNT 
                 1269 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGANGNNT 
                 1270 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNANTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGANTNNA 
                 1271 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNANTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGANTNNC 
                 1272 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANTCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGANTNNT 
                 1273 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGATCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGATCNNG 
                 1274 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCTCATCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGATGAGG 
                 1275 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCCCATCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGATGGGG 
                 1276 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCATCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGATGNNA 
                 1277 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCATCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGATGNNC 
                 1278 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCACATCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGATGTGG 
                 1279 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNAATCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGATTNNG 
                 1280 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCAANNG 
                 1281 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACACG 
                 1282 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACATG 
                 1283 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACGCG 
                 1284 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACGTG 
                 1285 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACNGG 
                 1286 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACNNA 
                 1287 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACNNC 
                 1288 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACNNT 
                 1289 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACTCG 
                 1290 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAGTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCACTTG 
                 1291 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGAGAG 
                 GGCAGNCA 
                 1292 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCAGNCC 
                 1293 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCAGNGA 
                 1294 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCAGNGC 
                 1295 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCAGNNT 
                 1296 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCAGNTA 
                 1297 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCTGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCAGNTC 
                 1298 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATACG 
                 1299 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATATG 
                 1300 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATGCG 
                 1301 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATGTG 
                 1302 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATNGG 
                 1303 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATNNA 
                 1304 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATNNC 
                 1305 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATNNT 
                 1306 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGAATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATTCG 
                 1307 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAAATGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCATTTG 
                 1308 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGGGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCCCNCT 
                 1309 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGGGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCCCNGT 
                 1310 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGGGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCCCNTT 
                 1311 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNAGGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCCTNCT 
                 1312 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNAGGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCCTNGT 
                 1313 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANAGGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCCTNTT 
                 1314 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGCGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCGCNCT 
                 1315 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGCGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCGCNGT 
                 1316 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGCGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCGCNTT 
                 1317 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNACGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCGTNCT 
                 1318 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNACGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCGTNGT 
                 1319 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANACGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCGTNTT 
                 1320 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCNANNA 
                 1321 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCNANNC 
                 1322 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCNANNT 
                 1323 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTANCG 
                 1324 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTANGG 
                 1325 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTANTG 
                 1326 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTCNCA 
                 1327 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTCNCC 
                 1328 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTCNGA 
                 1329 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTCNGC 
                 1330 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTCNNT 
                 1331 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTCNTA 
                 1332 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTCNTC 
                 1333 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTGNCT 
                 1334 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTGNGT 
                 1335 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTGNTT 
                 1336 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTTNCA 
                 1337 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTTNCC 
                 1338 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTTNGA 
                 1339 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTTNGC 
                 1340 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNAAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTTNNT 
                 1341 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTTNTA 
                 1342 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAAGCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGCTTNTC 
                 1343 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGAANNG 
                 1344 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGACNGG 
                 1345 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGACNNA 
                 1346 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGACNNC 
                 1347 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGACNNT 
                 1348 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGAGNCA 
                 1349 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGAGNCC 
                 1350 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGAGNGA 
                 1351 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGAGNGC 
                 1352 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGAGNNT 
                 1353 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGAGNTA 
                 1354 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCTCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGAGNTC 
                 1355 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNATCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGATNGG 
                 1356 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGATNNA 
                 1357 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGATNNC 
                 1358 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNATCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGATNNT 
                 1359 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGGCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGCCNCT 
                 1360 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGGCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGCCNGT 
                 1361 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGGCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGCCNTT 
                 1362 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNAGCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGCTNCT 
                 1363 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNAGCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGCTNGT 
                 1364 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANAGCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGCTNTT 
                 1365 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNGCCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGGCNCT 
                 1366 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNGCCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGGCNGT 
                 1367 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANGCCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGGCNTT 
                 1368 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNACCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGGTNCT 
                 1369 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNACCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGGTNGT 
                 1370 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANACCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGGTNTT 
                 1371 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGNANNA 
                 1372 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGNANNC 
                 1373 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNCCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGNANNT 
                 1374 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTANCG 
                 1375 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTANGG 
                 1376 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTANTG 
                 1377 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTCNCA 
                 1378 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTCNCC 
                 1379 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTCNGA 
                 1380 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTCNGC 
                 1381 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTCNNT 
                 1382 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTCNTA 
                 1383 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTCNTC 
                 1384 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTGNCT 
                 1385 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTGNGT 
                 1386 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTGNTT 
                 1387 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTTNCA 
                 1388 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTTNCC 
                 1389 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTTNGA 
                 1390 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTTNGC 
                 1391 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNAACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTTNNT 
                 1392 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTTNTA 
                 1393 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAACCCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGGTTNTC 
                 1394 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAANNG 
                 1395 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTACNNA 
                 1396 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTACNNC 
                 1397 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTACNNG 
                 1398 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGTCTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGACG 
                 1399 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCATCTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGATG 
                 1400 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGCCTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGGCG 
                 1401 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCACCTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGGTG 
                 1402 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGNGG 
                 1403 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGNNA 
                 1404 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGNNC 
                 1405 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGNNT 
                 1406 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGACTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGTCG 
                 1407 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCAACTACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTAGTTG 
                 1408 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNATACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTATNNA 
                 1409 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNATACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTATNNC 
                 1410 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTATNNG 
                 1411 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCANCG 
                 1412 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCANGG 
                 1413 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCANTG 
                 1414 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCCNCA 
                 1415 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCCNCC 
                 1416 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCCNGA 
                 1417 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCCNGC 
                 1418 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCCNTA 
                 1419 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCCNTC 
                 1420 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCGNCT 
                 1421 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCGNGT 
                 1422 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCGNTT 
                 1423 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNAGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCTNCA 
                 1424 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNAGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCTNCC 
                 1425 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNAGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCTNGA 
                 1426 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNAGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCTNGC 
                 1427 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANAGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCTNTA 
                 1428 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANAGACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTCTNTC 
                 1429 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNTCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGANCG 
                 1430 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNTCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGANGG 
                 1431 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANTCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGANTG 
                 1432 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNGCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGCNCA 
                 1433 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNGCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGCNCC 
                 1434 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNGCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGCNGA 
                 1435 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNGCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGCNGC 
                 1436 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANGCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGCNTA 
                 1437 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANGCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGCNTC 
                 1438 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAGNCCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGGNCT 
                 1439 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATACNCCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGGNGT 
                 1440 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATAANCCACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGGNTT 
                 1441 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNACACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGTNCA 
                 1442 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNACACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGTNCC 
                 1443 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNACACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGTNGA 
                 1444 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNACACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGTNGC 
                 1445 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANACACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGTNTA 
                 1446 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANACACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTGTNTC 
                 1447 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNTNACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTNANNA 
                 1448 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNTNACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTNANNC 
                 1449 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNTNACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTNANNT 
                 1450 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTNCNNT 
                 1451 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTNTNNT 
                 1452 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNTAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTANNG 
                 1453 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTCNGG 
                 1454 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTCNNA 
                 1455 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTCNNC 
                 1456 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTGNCAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTGNCA 
                 1457 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGGNCAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTGNCC 
                 1458 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTCNCAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTGNGA 
                 1459 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGCNCAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTGNGC 
                 1460 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTGNNT 
                 1461 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTANCAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTGNTA 
                 1462 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGANCAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTGNTC 
                 1463 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNAAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTTNGG 
                 1464 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNAAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTTNNA 
                 1465 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNAAACCGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GGTTTNNC 
                 1466 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNACGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 GTNNNNNN 
                 1467 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNTAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TANNNNNN 
                 1468 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNGAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TCNNNNNN 
                 1469 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNTCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGANNNNN 
                 1470 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGCAGNNG 
                 1471 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGCNANNN 
                 1472 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNGNGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGCNCNNN 
                 1473 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCNGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGCNGNNA 
                 1474 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCNGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGCNGNNC 
                 1475 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGCNGNNT 
                 1476 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNANGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGCNTNNN 
                 1477 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCAGCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGCTGNNG 
                 1478 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGTCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGACNNG 
                 1479 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNCTCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGAGNNG 
                 1480 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNATCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGATNNG 
                 1481 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGGCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGCCNNG 
                 1482 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNAGCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGCTNNG 
                 1483 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTTGCCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGCAAG 
                 1484 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTCGCCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGCGAG 
                 1485 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNGCCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGCNCG 
                 1486 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNGCCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGCNGG 
                 1487 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANGCCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGCNTG 
                 1488 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTAGCCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGCTAG 
                 1489 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTTACCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGTAAG 
                 1490 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTCACCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGTGAG 
                 1491 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNACCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGTNCG 
                 1492 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNACCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGTNGG 
                 1493 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANACCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGTNTG 
                 1494 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTAACCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGGTTAG 
                 1495 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNTNCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNANNN 
                 1496 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNGNCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNCNNA 
                 1497 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNGNCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNCNNC 
                 1498 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNGNCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNCNNT 
                 1499 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNCNCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNGNNA 
                 1500 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNCNCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNGNNC 
                 1501 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNCNCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNGNNT 
                 1502 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATTNNANCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNTNNA 
                 1503 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATGNNANCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNTNNC 
                 1504 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATANNANCCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGNTNNT 
                 1505 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNGACCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGTCNNG 
                 1506 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTTCACCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGTGAAG 
                 1507 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTCCACCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGTGGAG 
                 1508 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCGNCACCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGTGNCG 
                 1509 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCCNCACCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGTGNGG 
                 1510 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCANCACCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGTGNTG 
                 1511 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCTACACCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGTGTAG 
                 1512 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATCNNAACCAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGGTTNNG 
                 1513 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNACAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TGTNNNNN 
                 1514 
               
               
                   
               
               
                 CAAGCAGAAGACGGCATACGAGATNNNNNNAAGTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG 
                 TTNNNNNN 
                 1515 
               
               
                   
               
            
           
         
       
     
     5. Discussion 
     In the examples, the index sequence and the number of reads of the primer used for a next-generation sequencer (Illumina) were analyzed. As a result, no apparent correlation was observed in the primer P5 used for a next-generation sequencer, but an apparent correlation was observed in the primer P7 used for a next-generation sequencer (the correlational coefficient: 0.9 or higher). By calculating the putative number of reads based on the types of nucleotides constituting the index sequence using the estimation formula prepared by the GLMNET LASSO method, in particular, the primers P7 used for a next-generation sequencer with different index sequences were found to be classified into three groups based on the putative number of reads and the measured number of reads. Specifically, Group 1, which provides the number of reads as small as 15,000 or lower and is considered difficult to be subjected to analysis using the next-generation sequencer, was identified. The maximal putative number of reads of the primers P7 used for a next-generation sequencer of Group 1 was 20,051.8. In the examples, accordingly, all the index sequences providing the putative number of reads, which was calculated with the use of the estimation formula using the types of nucleotides constituting the index sequence as parameters, of 20,052 or higher were selected (Table 6). The primers P7 used for a next-generation sequencer comprising the selected index sequences (Table 6) are considered to be capable of providing a large number of reads when applied to the next-generation sequencer. 
     Since the putative number of reads provided by the primers P7 used for a next-generation sequencer of Group 3 was 50,000 or higher, all the index sequences providing the putative number of reads, which was calculated with the use of the estimation formula using the types of nucleotides constituting the index sequence as parameters, of 50,000 or higher were selected (Table 7). The primers P7 used for a next-generation sequencer comprising the selected index sequences (Table 7) are considered to be capable of providing a larger number of reads when applied to the next-generation sequencer. 
     INDUSTRIAL APPLICABILITY 
     With the use of the primer P7 used for a next-generation sequencer designed in the examples, data can be obtained with the use of the next-generation sequencer with higher stability.