Patent Publication Number: US-2020291131-A1

Title: Bispecific her2 antibodies and methods of use

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application is a Division of U.S. patent application Ser. No. 15/186,098, filed Jun. 17, 2016, which is a Continuation of International Patent Application No. PCT/EP2014/078375, filed Dec. 18, 2014, now publication WO 2015/091738, which claims the benefit of priority to European Patent Application No. 13198819.8, filed Dec. 20, 2013, each of which is incorporated herein by reference in its entirety. 
    
    
     SEQUENCE LISTING 
     The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jan. 3, 2020, is named P31865-US-1_Sequence_Listing.txt and is 253,352 bytes in size. 
     FIELD OF THE INVENTION 
     The present invention relates to bispecific HER2 antibodies, novel HER2 variants, methods for their production, pharmaceutical compositions containing said antibodies, and uses thereof. 
     BACKGROUND 
     Antibodies specific for tumor-associated antigens are a valuable approach in cancer therapy because they mediate selective destruction of tumor cells, while leaving healthy cells and tissues undamaged. 
     Members of the ErbB family of receptor tyrosine kinases are important mediators of cell growth, differentiation and survival. The receptor family includes four distinct members, including epidermal growth factor receptor (EGFR or ErbB1), HER2 (ErbB2 or pl85“e”), HER3 (ErbB3) and HER4 (ErbB4 or tyro2). HER2 is a transmembrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is a promising target for treatment of breast cancer as it was found to be overexpressed in about one-quarter of breast cancer patients (Bange et al, 2001, Nature Medicine 7:548). 
     The murine monoclonal antibody 4D5 is targeting HER2 specifically in HER2 overexpressing cancer cells, while having no effect on cells expressing physiological levels of HER2. The humanized (4D5) monoclonal antibody (hu4D5) is commercially known as the drug Herceptin® (trastuzumab, rhuMAb HER2, U.S. Pat. No. 5,821,337), which gained FDA marketing approval in late 1998. 
     Herceptin was the first monoclonal antibody developed for the treatment of HER2-positive breast cancer and has increased survival times for patients so that they are now the same as for patients with HER2-negative breast cancer. Before Herceptin treatment, shorter survival outcomes were expected for patients diagnosed with HER2-positive breast cancer, compared to patients with HER2-negative disease. In the CLEOPATRA study, PERJETA in combination with Herceptin and chemotherapy has shown the extension of survival times for patients with this aggressive disease even further than Herceptin. 
     Pertuzumab (PERJETA™, rhuMab 2C4, U.S. Pat. No. 7,862,817) is a humanized monoclonal antibody, which is designed specifically to prevent the HER2 receptor from pairing (dimerising) with other HER receptors (EGFR/HER1, HER3 and HER4) on the surface of cells, a process that is believed to play a role in tumor growth and survival. The combination of PERJETA, Herceptin and chemotherapy is thought to provide a more comprehensive blockade of HER signaling pathways. PERJETA is approved in combination with Herceptin (trastuzumab) and docetaxel in adult patients with HER2-positive metastatic or locally recurrent unresectable breast cancer and gained FDA approval for neoadjuvant breast cancer treatment in September 2013. Pertuzumab binds to domain II of HER2, essential for dimerization, while Ttrastuzumab binds to extracellular domain IV of HER2. 
     Li et al (Cancer Research. 2013) describe bispecific, bivalent antibodies to ErbB2 that overcome trastuzumab resistance. The bispecific, bivalent antibodies described therein are based on the native Trastuzumab and Pertuzumab sequences. 
     Surprisingly the inventors of the present application found that optimizing the native Trastuzumab and Pertuzumab sequences and combining these optimized variants in two different improved bispecific, monovalent antibody formats leads to improved properties as compared to the combination of the monospecific antibodies rhuMab 2C4 and hu 4D5. Further the antibodies are superior to the bivalent antibody formats disclosed in Li et al, as they are monovalent and have the same molecular weight as the two monospecific antibodies Pertuzumab and Trastuzumab. Hence the new bispecific format combines the superior characteristics of the bispecific HER2 antibodies known in the art with the advantages of a classical monospecific antibody: The novel bispecific HER2 antibodies of the present invention are monovalent for the two different HER2 epitopes, resulting in the same avidity effect as the bivalent parental antibodies. In contrast, tetravalent antibodies may differ in their avidity for HER2 on cells. The avidity effect of the novel bispecific HER2 antibodies may result in a superior safety window on cell types with low HER2 expression such as in normal tissues or cardiac tissues where inhibition of HER2 and/or ADCC may not be desired. 
     Furthermore, the bispecific antibodies described herein have the same diffusion constants as the bivalent parental antibodies due to their natural IgG architecture that matches to the diffusion constant of the parental 150 KD IgG1 antibody. Due to the natural IgG architecture furthermore the risk for immunogenicity and the formation of anti-drug antibodies can be expected to be reduced. Last but not least as compared to tetravalent bispecific antibodies the risk for the formation of immune complexes with shed HER2 extracellular domain is reduced by being monovalent and comparable to the parental antibodies. Immune complexes may result in the enhanced immunogenicity of the complex taken up by antigen presenting cells and ultimately can induce kidney toxicity if immune complexes are deposited in the kidney. 
     In one aspect of the invention a monovalent bispecific antibody is provided, wherein one of the Fab fragments of an IgG molecule is replaced by a crossover Fab fragment. Crossover Fab fragments are Fab fragments wherein either the variable regions or the constant regions of the heavy and light chain are exchanged. Bispecific antibody formats comprising crossover Fab fragments have been described, for example, in WO2009080252, WO2009080253, WO2009080251, WO2009080254, WO2010/136172, WO2010/145792 and WO2013/026831. The native Trastuzumab sequences has been optimized in their CDRs to improve the stability of the antibody CDRs against spontaneous chemical modification, the resulting sequences framework-grafted to avoid mispairing, and the bispecific antibody glycoengineered, resulting in highly potent bispecific antibodies that specifically bind to HER2 with enhanced FcgRIII binding resulting in enhanced recruitment of immune effector cells such as NK cells or monocytes/macrophages; finally they can be produced with high yield and only low percentage of side products comparable to the conventional parental Her2 antibodies. In the case of the HER2 bispecific CrossMAb antibody chain mispairing of light chains resulting from the fact that both pertuzumab and trastuzumab are based on a comparable framework region has been overcome by grafting the CDRs on a completely novel antibody framework. 
     In another aspect of the invention monovalent bispecific antibodies specifically binding to the extracellular domains IV and II of HER2 are provided wherein the two binding moieties comprise identical light chains based on a consensus of the parental trastuzumab and pertuzumab light chains and the corresponding pertuzumab heavy chain has been remodeled. The use of this so-called ‘common light chain’ principle, i.e. combining two binders that share one light chain but still have separate specificities, prevents light chain mispairing and in this particular case retains the epitope specificity of the parental antibodies. As a consequence, there are less side products during production, facilitating the homogenous preparation of HER2 bispecific antigen binding molecules at high yields. Surprisingly the inventors of the present invention found that the bispecific HER2 antibodies in the monovalent common light chain format have an increased affinity to the pertuzumab epitope, and show superior inhibitory effects on cell proliferation and induction of cell dependent cytotoxicity (CDC) as compared to the combination of the parental antibodies. Complement dependent cytotoxicity (CDC) is very important for the optimal therapeutic monoclonal antibodies (mAb) function and is totally conserved even after a chemotherapy treatment. However, this activity is generated by some antibodies but not all of them. 
     SUMMARY 
     The present invention relates to bispecific antibodies specifically binding to HER2 comprising a first antigen binding site specific for the extracellular domain II of HER2 and a second antigen binding site specific for the extracellular domain IV of HER2, wherein the bispecific antibody is monovalent for both the extracellular domain II and IV of HER2. In one embodiment the bispecific antibody induces complement-dependent cytotoxicity (CDC) to a higher degree than the combination of Pertuzumab or Trastuzumab. In one such embodiment the complement dependent cytotoxicity of the bispecific antibody is determined by a LDH assay or a complement assay and compared to the complement dependent cytotoxicity of the combination of Pertuzumab and Trastuzumab as determined by the same assay. In one embodiment the complement dependent cytotoxicity is determined in vitro on cancer cells, preferably on breast cancer cells. In one aspect the bispecific antibody specifically binding to HER2, comprises a first Fab molecule capable of specific binding to extracellular domain II of HER2 and a second Fab molecule capable of specific binding to extracellular domain IV of HER2, wherein the sequence of the variable light chain of the first Fab molecule is identical to the sequence of the variable light chain of the second Fab molecule. In one aspect the bispecific antibody specifically binding to HER2 comprises (a) a first heavy chain comprising a heavy chain CDR1 selected from the group consisting of SEQ ID NO: 55, SEQ ID NO: 58 and SEQ ID NO: 14; a heavy chain CDR 2 selected from the group consisting of SEQ ID NO: 77; SEQ ID NO: 15 and SEQ ID NO: 60 and a heavy chain CDR 3 selected from the group consisting of SEQ ID NO: 56 or SEQ ID NO: 59 and SEQ ID NO: 16, and (b) a second heavy chain comprising a heavy chain CDR1 of SEQ ID NO: 20, a heavy chain CDR2 of SEQ ID NO: 29 and a heavy chain CDR3 selected from the group consisting of SEQ ID NO: 30 and SEQ ID NO: 79; and (c) a first and a second light chain, wherein the variable light chains of the first and second light chain comprise the CDRs of SEQ ID NO: 89, SEQ ID NO: 90 and SEQ ID NO: 19. In one aspect the bispecific antibody specifically binding to HER2 comprises two variable light chains comprising an amino acid sequence of SEQ ID NO: 54, a first heavy chain comprising a variable heavy chain comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 64, SEQ ID NO: 70 and SEQ ID NO: 68, and a second heavy chain comprising a variable heavy chain comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 92 and SEQ ID NO: 117. In one aspect the bispecific antibody specifically binding to HER2 comprises a first Fab molecule capable of specific binding to extracellular domain II of HER2 and a second Fab molecule capable of specific binding to extracellular domain IV of HER2, wherein either the variable regions or the constant regions of the heavy and light chain of at least one Fab fragment are exchanged. In one aspect the bispecific antibody specifically binding to HER2 comprises a first Fab molecule comprising a heavy chain CDR1 of SEQ ID NO: 14, a heavy chain CDR2 of SEQ ID NO: 15 and a heavy chain CDR3 of SEQ ID NO: 16; and a light chain CDR1 of SEQ ID NO: 11; a light chain CDR2 of SEQ ID NO: 12 and a light chain CDR3 of SEQ ID NO: 13, and a second Fab molecule comprising a heavy chain CDR1 of SEQ ID NO: 20; a heavy chain CDR2 of SEQ ID NO: 108; a heavy chain CDR3 of SEQ ID NO: 79; and a light chain CDR1 of SEQ ID NO: 107, a light chain CDR2 of SEQ ID NO: 18 and a light chain CDR3 of SEQ ID NO: 19. In one aspect the bispecific antibody specifically binding to HER2 comprises a first Fab molecule comprising a heavy chain CDR1 of SEQ ID NO: 14, a heavy chain CDR2 of SEQ ID NO: 15 and a heavy chain CDR3 of SEQ ID NO: 16; and a light chain CDR1 of SEQ ID NO: 11; a light chain CDR2 of SEQ ID NO: 12 and a light chain CDR3 of SEQ ID NO: 13, and a second Fab molecule comprising a heavy chain CDR1 of SEQ ID NO: 20, a heavy chain CDR2 of SEQ ID NO: 29, and a heavy chain CDR3 selected from the group consisting of SEQ ID NO: 79, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 87, SEQ ID NO: 88; and a light chain CDR1 selected from the group consisting of SEQ ID NO: 104, SEQ ID NO: 103 and SEQ ID NO: 158; a light chain CDR2 of SEQ ID NO: 18 and a light chain CDR3 of SEQ ID NO: 19. In one aspect the bispecific antibody specifically binding to HER2 comprises a first Fab molecule comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 22 and a variable light chain comprising an amino acid sequence of SEQ ID NO: 24 and wherein a second Fab molecule comprising an amino acid sequence of SEQ ID NO: 105 and a light chain variable region comprising an amino acid sequence of SEQ ID NO: 106. 
     In a second object the present invention relates to a pharmaceutical composition comprising a bispecific antibody of the present invention. 
     In a third object the present invention relates to a bispecific antibody of the present invention for the treatment of cancer. In another embodiment, use of the bispecific antibody as a medicament is provided. Preferably said use is for the treatment of cancer. 
     In further objects the present invention relates to a nucleic acid sequence comprising a sequence encoding a heavy chain of a bispecific antibody of the present invention, a nucleic acid sequence comprising a sequence encoding a light chain of a bispecific antibody of the present invention, an expression vector comprising a nucleic acid sequence of the present invention and to a prokaryotic or eukaryotic host cell comprising a vector of the present invention. In addition a method of producing an antibody comprising culturing the host cell so that the antibody is produced is provided. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIGS. 1A-1D : Schematic drawing of Trastuzumab and Pertuzumab bispecific antibodies in a 2+2 IgG-scFv format. The antibodies are bivalent for each antigen binding site and are able to bind two different paratopes in the ErbB2/HER2 receptor (antigen1=trastuzumab specificity, i.e. extracellular domain IV of HER2; antigen2=pertuzumab specificity extracellular domain II of HER2) ( 1 A): The single chain Fv (scFv) is fused C-terminally to the heavy chain in the order VH-VL (TvAB12, SEQ ID NOs 123 and 124). ( 1 B): The single chain Fv (scFv) is fused N-terminally to the light chain in the order VL-VH (TvAB13, SEQ ID NOs 125 and 126). ( 1 C) The single chain Fv (scFv) is fused C-terminally to the light chain in the order VL-VH (TvAB16: SEQ ID NOs 127 and 128, TvAB20: SEQ ID NOs 131 and 132). ( 1 D): The single chain Fv (scFv) is fused C-terminally to the heavy chain in the order VL-VH (TvAB17: SEQ ID NOs 129 and 130). 
         FIGS. 2A and 2B : Purification of Trastuzumab and Pertuzumab bispecific antibodies in a 2+2 IgG-scFv format. ( 2 A): Size-exclusion purification of TvAb12 (SEQ ID NOs 123 and 124) on a 26/60 Superdex 200 column. ( 2 B): SDS-Page analysis of main peak fraction originating from size-exclusion chromatography (NR=non-reducing, R=reducing conditions). 
         FIGS. 3A and 3B : Purification of Trastuzumab and Pertuzumab bispecific antibodies in a 2+2 IgG-scFv format. ( 3 A): Size-exclusion purification of TvAb16 (SEQ ID NOs 127 and 128) on a 26/60 Superdex 200 column. ( 3 B): SDS-Page analysis of main peak fraction originating from size-exclusion chromatography (NR=non-reducing, R=reducing conditions). 
         FIGS. 4A and 4B : Purification of Trastuzumab and Pertuzumab bispecific antibodies in a 2+2 IgG-scFv format. ( 4 A): Size-exclusion purification of TvAb20 (SEQ ID NOs 131 and 132) on a 26/60 Superdex 200 column. Main product peak marked with “1”. ( 4 B) SDS-Page analysis of main peak fraction originating from size-exclusion chromatography (NR=non-reducing, R=reducing conditions). 
         FIGS. 5A and 5B : Off-rates of Trastuzumab variants as determined by SPR method (ProteOn instrument) after incubating the samples for 1, 2, or 3 months at 40° in buffer 40 mM Histidin, 150 mM NaCl, pH5.0. The off rates of the variant does not change over the investigated time period. “602”: D98E mutation in heavy chain and T31V mutation in light chain. 
         FIGS. 6A-6C : Off-rates of Trastuzumab variants as determined by SPR method (ProteOn instrument) after incubating the samples for 1, 2, or 3 months at 40° C. in 40 mM Histidin, 150 mM NaCl, at different pH. The off rates of the N30S variant were very slow, and therefore contain a high degree of uncertainty. “602”: D98E mutation in heavy chain and T31V mutation in light chain, “N30T”: D98E mutation in heavy chain and N30T mutation in light chain, “N30S”: D98E mutation in heavy chain and N30S mutation in light chain. ( 6 A): pH5.0. ( 6 B): pH6.0, ( 6 C): pH7.4. 
         FIGS. 7A and 7B : Binding of Trastuzumab and Trastuzumab stabilization variants after stress to KPL-4 cells. Trastuzumab and 3 different stabilized Trastuzumab variants were incubated for one, two and three month in buffer with different pH values at 40° C. The stressed antibodies were tested compared to the antibody at time point zero for binding to KPL-4 cells by flow cytometry. “602”: D98E mutation in heavy chain and T31V mutation in light chain, “N30T”: D98E mutation in heavy chain and N30T mutation in light chain, “N30S”: D98E mutation in heavy chain and N30S mutation in light chain. 
         FIG. 8 : Binding of Trastuzumab and Trastuzumab stabilization variants after stress to KPL-4 cells. Trastuzumab and the 2 stabilization variants GA602 (D98E mutation in heavy chain and T31V mutation in light chain) and GA603 (D98E mutation in heavy chain and T31V mutation in light chain and FcRN mutation T307Q and N434A) were incubated for one, two and three month in buffer 1 (40 mM Histidin 150 mM NaCl, pH5.0) or buffer 2 (2. 40 mM Histidin 150 mM NaCl, pH6.0) at 40° C. The stressed antibodies were tested compared to the antibody at time point zero for binding to KPL-4 cells by flow cytometry. 
         FIG. 9 : ADCC induction with Trastuzumab, GA602 and GA603 after stress on KPL-4 cells. Trastuzumab and the 2 stabilization variants GA602 (D98E mutation in heavy chain and T31V mutation in light chain) and GA603 (D98E mutation in heavy chain and T31V mutation in light chain and FcRN mutation T307Q and N434A) were incubated for one, two and three month in buffer 1 (40 mM Histidin 150 mM NaCl, pH 5.0) or buffer 2 (2. 40 mM Histidin 150 mM NaCl, pH6.0) at 40° C. The stressed antibodies were tested compared to the antibody at time point zero for ADCC induction after 4 h on KPL-4 cells. 
         FIGS. 10A and 10B : Schematic drawing of Trastuzumab and Pertuzumab bispecific antibodies in a 1+1 format. ( 10 A): single chain Fab (scFab) based molecules ( 10 B): cross-over Fab (xFab) based molecules. 
         FIGS. 11A and 11B : Purification of CrossMab-XPer (SEQ ID NOs 109, 110, 96, 86). ( 11 A): SDS-PAGE showing the purified antibody molecule under reduced and non-reduced conditions. ( 11 B): HP-SEC analysis of purified CrossMab-XPer. 
         FIG. 12 : Q-TOF mass spectrometry comparison of the spectra of CrossMab-XTra (top, SEQ ID NOs 119, 120, 121, 122) and CrossMab-CDRG (bottom, SEQ ID NOs 109, 110, 111, 112) estimating the integrity and purity of the antibody molecules. 
         FIGS. 13A and 13B : Proliferation inhibition by non-glycoengineered HER2 CrossMab (SEQ ID NOs 119, 120, 121, 122) after 5 days of incubation as measured in an AlamarBlue® assay. ( 13 A) BT474 cells ( 13 B) N87 cells. 
         FIGS. 14A-14C : ADCC induced by different HER2 specific antibodies using ( 14 A) KPL-4, ( 14 B) T47D and ( 14 C) Calu-3 as target cells (E:T=25:1, effectors human PBMCs, incubation time 4 h). “HER2 crossmab wt”: SEQ ID NOs 119, 120, 121, 122, non glycoengineered; “HER2 crossmab g2”: SEQ ID NOs 119, 120, 121, 122, glycoengineered. 
         FIGS. 15A-15C : Antitumor activity of different anti-Her2 antibodies in the Calu3 non-small cell lung cancer xenograft (Experiment: BispecHer2_PZ_Calu3_001). SCID beige mice with Calu3 xenograft tumors were treated i.p. once weekly at the indicated dosages for 7 weeks. Xolair a humanized IgG1 antibody targeting human IgE was used as a control. Statistical analysis based on medians at endpoint (day 85) reveals that compared to Xolair the bispecific HER2 antibodies suppressed tumor growth by 87.5% (s.); OmniE (SEQ ID NOs 145, 146) by 43.7% (n.s.); Crossmab 003 (SEQ ID NOs 119, 120, 121, 122, non glycoengineered) by 92.1% (s.); TvAb12 (SEQ ID NOs 123 and 124) by 59.8% (n.s.) and TvAb20 (SEQ ID NOs 131 and 132) by 12.6% (n.s.). Tumor growth curves are depicted as mean+/−SEM (n=8 in each group). 
         FIGS. 16A-16C : Antitumor activity of different anti-Her2 antibodies in the KPL-4 breast cancer xenograft (Experiment: Bispec.Her2_PZ_KPL-4_002). SCID beige mice with KPL-4 xenograft tumors were treated i.p. once weekly at the indicated dosages for 5 weeks. Xolair a humanized IgG1 antibody targeting human IgE was used as a control. Statistical analysis based on medians at endpoint (day 59) reveals that compared to Xolair the bispecific HER2 antibodies suppressed tumor growth by 120.8% (s.); Crossmab_003 (SEQ ID NOs 119, 120, 121, 122, non glycoengineered) by 120.6% (s.); TvAb12 (SEQ ID NOs 123 and 124) by 70.1% (s.); TvAb20 (SEQ ID NOs 131 and 132) by 83.4% (s.). OmniE (SEQ ID NOs 145, 146) had no significant effect on tumor growth. Tumor growth curves are depicted as mean+/−SEM (n=9 in each group). 
         FIGS. 17A-17C : Antitumor activity of anti-Her2_005 crossmab antibody (SEQ ID NOs 119, 120, 121, 122, non glycoengineered) in the KPL-4 breast cancer xenograft (Experiment: Bispec.Her2_PZ_KPL-4_003). SCID beige mice with KPL-4 xenograft tumors were treated i.p. once weekly with escalating dosages of the crossmab ranging from 1 to 20 mg/kg for 5 weeks. Xolair a humanized IgG1 antibody targeting human IgE was used as a control. Statistical analysis based on medians at endpoint (day 70) reveals that compared to Xolair the bispecific HER2 antibodies suppressed tumor growth by 121.8% (s.); The Her2 crossmab_005 suppressed tumor growth at a dosage of 1 mg/kg by 25.1% (n.s.); at 5 mg/kg by 112.3% (s.); at 10 mg/kg by 109.5% (s.) and by 20 mg/kg by 121.8% (s.). Tumor growth curves are depicted as mean+/−SEM (n=10 in each group). 
         FIGS. 18A-18C : Antitumor activity of different anti-Her2 antibodies in the KPL-4 breast cancer xenograft (Experiment: Bispec.Her2_PZ_KPL-4_009). SCID beige mice with KPL-4 xenograft tumors were treated i.p. once weekly with the different compounds for 4 weeks. Xolair a humanized IgG1 antibody targeting human IgE was used as a control. Statistical analysis based on medians at endpoint (day 70) reveals that compared to Xolair the bispecific HER2 antibodies (each dosed at 5 mg/kg) suppressed tumor growth by 83.2% (s.) and both given at a dosage of 10 mg/kg each by 109.5% (s.). TvAb 16 (SEQ ID NOs 127 and 128) given at two different dosages (5 mg/kg and 10 mg/kg) had no significant anti-tumoral effect. TvAb20 (SEQ ID NOs 131 and 132), at a dosage of 5 mg/kg, suppressed tumor growth by 75.3% (s.) and at a dosage of 10 mg/kg by 59.8% (n.s.). Tumor growth curves are depicted as mean+/−SEM (n=10 in each group). 
         FIG. 19 : SPR analysis of initial Pertuzumab/Trastuzumab hybrid light chains. SPR-based kinetic analyses of Pertuzumab, Trastuzumab, and sequence combinations with the initial Pertuzumab hybrid LCs harboring amino acid residues of the Trastuzumab LCDR3 region. Smooth lines represent a global fit of the data to a 1:1 interaction model. PertuzumabTrasL3: SEQ ID No: 26, PertuzumabTras Y91H: SEQ ID No: 28. 
         FIG. 20 : SPR analysis of the Pertuzumab and Trastuzumab HCs in combination with the newly identified common light chain Pertuzumab (Tras.L3)(QM), SEQ ID No: 54. Shown is the binding of both antibodies to Her2 at different concentrations. Smooth lines represent a global fit of the data to a 1:1 interaction model. 
         FIG. 21 : Characterization of the affinity-matured Pertuzumab clones identified by phage display. SPR analysis of the identified affinity-matured clones. Shown is the binding of bacterial Fabs to Her2 at different concentrations. Smooth lines represent a global fit of the data to a 1:1 interaction model. B2: SEQ ID No: 66, D1: SEQ ID No: 62, E1: SEQ ID No: 68, C8: SEQ ID No: 72, G2: SEQ ID No: 70, A1: SEQ ID No: 74. 
         FIG. 22 : Schematic drawing of the bi-specific HER2 antibodies with a common light chain. 
         FIGS. 23A-23C : Purification and analytical characterization of the bi-specific HER2 antibodies with a common light chain. The purification method involved an affinity step (protein A) followed by size exclusion chromatography (Superdex 200, GE Healthcare). The final product was analyzed and characterized by analytical size exclusion chromatography (Superdex 200 column). ( 23 A): comprising D1der (SEQ ID NO: 64), ( 23 B): comprising G2 (SEQ ID NO: 70), ( 23 C): comprising E1 (SEQ ID NO: 68). 
         FIG. 24 : SPR analysis of the Her2 knock-out variants. Shown are the sensograms of Trastuzumab and Pertuzumab binding to both knock-out variants. Smooth lines represent a global fit of the data to a 1:1 interaction model. 
         FIG. 25 : Binding of bi-specific HER2 antibodies with a common light chain clone variants to KPL-4 cells. KPL-4 cells were stained with increasing concentrations of the indicated antibodies. The antibodies were detected with a FITC labeled anti-human secondary and the fluorescence was determined by flow cytometry. “Herceptarg CLC D1-der”: SEQ ID NOs 64, 54, 92, “Herceptarg CLC G2/2”: SEQ ID NOs 70, 54, 92, “Herceptarg CLC E1/1”: SEQ ID NOs 68, 54, 92; “GA 604”: SEQ ID NOs 109, 110, 111, 112. 
         FIGS. 26A-26C : Proliferation inhibition of BT474, N87, and SkBr3 cells with bi-specific HER2 antibodies with common light chain clone variants. BT474 ( 26 A), N87 ( 26 B), and SkBr3 ( 26 C) cells were treated with the three different Herceptarg variants. As controls Trastuzumab, Pertuzumab and the combination of both were included. After 5 days, proliferation inhibition was determined with CellTiter Glo. “Herceptarg CLC D1-der”: SEQ ID NOs 64, 54, 92, “Herceptarg CLC G2/2”: SEQ ID NOs 70, 54, 92, “Herceptarg CLC E1/1”: SEQ ID NOs 68, 54, 92; “GA 604”: SEQ ID NOs 109, 110, 111, 112. 
         FIGS. 27A and 27B : Killing of KPL-4 cells and MDA-MB 231 with bi-specific HER2 antibodies with common light chain variants. ( 27 A) Antibody dependent killing of KPL-4 cells with PBMCs (E:T 25:1) or was determined by measuring LDH release after 4 h. ( 27 B) Antibody dependent killing of MDA-MB 231 cells with PBMCs (E:T 5:1) was determined by measuring LDH release after 24 h. “Herceptarg CLC D1-der”: SEQ ID NOs 64, 54, 92, “Herceptarg CLC G2/2”: SEQ ID NOs 70, 54, 92, “Herceptarg CLC E1/1”: SEQ ID NOs 68, 54, 92; “GA 604”: SEQ ID NOs 109, 110, 111, 112. 
         FIG. 28 : Proliferation inhibition of BT474 cells with bi-specific HER2 antibodies with common light chain clone variants. BT474 cells were treated with the different Herceptarg variants. As controls Trastuzumab, Pertuzumab and the combination of both were included. After 6 days, proliferation inhibition was determined with CellTiter Glo. “Herceptarg CLC D1-der wt”: SEQ ID NOs 64, 54, 92, Herceptarg CLC D1-der G2”: SEQ ID NOs 64, 54, 92 (glycoengineered variant) “Herceptarg CrossMab”: SEQ ID NOs 109, 110, 111, 112. 
         FIG. 29 : C1q binding of Her2 antibodies on BT-474 cells. BT474 cells were incubated with the three Herceptarg variants. As controls Trastuzumab, Pertuzumab and the combination of both were included. “Herceptarg CLC D1-der wt”: SEQ ID NOs 64, 54, 92, Herceptarg CLC D1-der G2”: SEQ ID NOs 64, 54, 92 (glycoengineered variant) “Herceptarg CrossMab”: SEQ ID NOs 109, 110, 111, 112. 
         FIG. 30 : CDC activation on BT-474 cells (LDH release). BT474 cells were incubated with the three Herceptarg variants. As controls Trastuzumab, Pertuzumab and the combination of both were included. “Herceptarg CLC D1-der wt”: SEQ ID NOs 64, 54, 92, Herceptarg CLC D1-der G2”: SEQ ID NOs 64, 54, 92 (glycoengineered variant) “Herceptarg CrossMab”: SEQ ID NOs 109, 110, 111, 112. 
         FIGS. 31A and 31B : CDC mediated killing of BT-474 cells (ACEA). BT474 cells were incubated with the three Herceptarg variants. As controls Trastuzumab, Pertuzumab and the combination of both were included. “Herceptarg CLC D1-der wt”: SEQ ID NOs 64, 54, 92, Herceptarg CLC D1-der G2”: SEQ ID NOs 64, 54, 92 (glycoengineered variant) “Herceptarg CrossMab”: SEQ ID NOs 109, 110, 111, 112. 
         FIG. 32 : In vivo activity of bispecific antibodies. Tumor volume in mouse xenograft models after treatment with different Her2 bispecific molecules (10 mg/kg) was compared to treatment with Trastuzumab, Pertuzumab and the combination of both. “Herceptarg”: SEQ ID NOs 64, 54, 92. “Control”: Xolair, a non Her2 binding antibody. 
     
    
    
     DETAILED DESCRIPTION OF EMBODIMENTS OF THE INVENTION 
     I. Definitions 
     Throughout the disclosure, the terms “ErbB2”, “ErbB2 receptor”, “c-Erb-B2”, and “HER2” are used interchangeably, and, unless otherwise indicated, refer to a native sequence ErbB2 human polypeptide, or a functional derivative thereof “ber2”, “erbB2” and “c-erb-B2” refer to the corresponding human gene. 
     An “acceptor human framework” for the purposes herein is a framework comprising the amino acid sequence of a light chain variable domain (VL) framework or a heavy chain variable domain (VH) framework derived from a human immunoglobulin framework or a human consensus framework, as defined below. An acceptor human framework “derived from” a human immunoglobulin framework or a human consensus framework may comprise the same amino acid sequence thereof, or it may contain amino acid sequence changes. In some embodiments, the number of amino acid changes are 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, or 2 or less. In some embodiments, the VL acceptor human framework is identical in sequence to the VL human immunoglobulin framework sequence or human consensus framework sequence. 
     “Affinity” refers to the strength of the sum total of noncovalent interactions between a single binding site of a molecule (e.g., an antibody) and its binding partner (e.g., an antigen). Unless indicated otherwise, as used herein, “binding affinity” refers to intrinsic binding affinity which reflects a 1:1 interaction between members of a binding pair (e.g., antibody and antigen). The affinity of a molecule X for its partner Y can generally be represented by the dissociation constant (Kd). Affinity can be measured by common methods known in the art, including those described herein. Specific illustrative and exemplary embodiments for measuring binding affinity are described in the following. 
     An “affinity matured” antibody refers to an antibody with one or more alterations in one or more hypervariable regions (HVRs), compared to a parent antibody which does not possess such alterations, such alterations resulting in an improvement in the affinity of the antibody for antigen. 
     The terms “a bispecific HER2 antibody” and “a bispecific antibody that specifically binds to HER2” are used interchangeably and refer to a bispecific antibody that is capable of binding HER2 on both extracellular domains II and IV, respectively, with sufficient affinity such that the antibody is useful as a diagnostic and/or therapeutic agent in targeting cells expressing HER2. In one embodiment, the extent of binding of a bispecific antibody that specifically binds to HER2 on both extracellular domains II and IV to an unrelated, non-HER2 protein is less than about 10% of the binding of the antibody to HER2 as measured, e.g., by a Enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR) based assays (e.g. Biacore) or flow cytometry (FACS). In certain embodiments, a bispecific antibody that specifically binds to HER2 has a dissociation constant (Kd) of ≤1 μM, ≤100 nM, ≤10 nM, ≤1 nM, ≤0.1 nM, ≤0.01 nM, or ≤0.001 nM (e.g. 10 −8  M or less, e.g. from 10 −8  M to 10 −13  M, e.g., from 10 −9  M to 10 −13  M). 
     The term “antibody” herein is used in the broadest sense and encompasses various antibody structures, including but not limited to monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies), and antibody fragments so long as they exhibit the desired antigen-binding activity. 
     An “antibody fragment” refers to a molecule other than an intact antibody that comprises a portion of an intact antibody that binds the antigen to which the intact antibody binds. Examples of antibody fragments include but are not limited to Fv, Fab, Fab′, Fab′-SH, F(ab′) 2 ; diabodies, cross-Fab fragments; linear antibodies; single-chain antibody molecules (e.g. scFv); and multispecific antibodies formed from antibody fragments. scFv antibodies are, e.g. described in Houston, J. S., Methods in Enzymol. 203 (1991) 46-96). In addition, antibody fragments comprise single chain polypeptides having the characteristics of a VH domain, namely being able to assemble together with a VL domain, or of a VL domain, namely being able to assemble together with a VH domain to a functional antigen binding site and thereby providing the antigen binding property of full length antibodies. 
     As used herein, “Fab fragment” refers to an antibody fragment comprising a light chain fragment comprising a VL domain and a constant domain of a light chain (CL), and a VH domain and a first constant domain (CH1) of a heavy chain. In one embodiment the bispecific antibodies of the invention comprise at least one Fab fragment, wherein either the variable regions or the constant regions of the heavy and light chain are exchanged. Due to the exchange of either the variable regions or the constant regions, said Fab fragment is also referred to as “cross-Fab fragment” or “xFab fragment” or “crossover Fab fragment”. Two different chain compositions of a crossover Fab molecule are possible and comprised in the bispecific antibodies of the invention: On the one hand, the variable regions of the Fab heavy and light chain are exchanged, i.e. the crossover Fab molecule comprises a peptide chain composed of the light chain variable region (VL) and the heavy chain constant region (CH1), and a peptide chain composed of the heavy chain variable region (VH) and the light chain constant region (CL). This crossover Fab molecule is also referred to as CrossFab (vLvH). On the other hand, when the constant regions of the Fab heavy and light chain are exchanged, the crossover Fab molecule comprises a peptide chain composed of the heavy chain variable region (VH) and the light chain constant region (CL), and a peptide chain composed of the light chain variable region (VL) and the heavy chain constant region (CH1). This crossover Fab molecule is also referred to as CrossFab (CLCH1) . 
     A “single chain Fab fragment” or “scFab” is a polypeptide consisting of an antibody heavy chain variable domain (VH), an antibody constant domain 1 (CH1), an antibody light chain variable domain (VL), an antibody light chain constant domain (CL) and a linker, wherein said antibody domains and said linker have one of the following orders in N-terminal to C-terminal direction: 
     a) VH-CH1-linker-VL-CL, b) VL-CL-linker-VH-CH1, c) VH-CL-linker-VL-CH1 or d) VL-CH1-linker-VH-CL; and wherein said linker is a polypeptide of at least 30 amino acids, preferably between 32 and 50 amino acids. Said single chain Fab fragments a) VH-CH1-linker-VL-CL, b) VL-CL-linker-VH-CH1, c) VH-CL-linker-VL-CH1 and d) VL-CH1-linker-VH-CL, are stabilized via the natural disulfide bond between the CL domain and the CH1 domain. In addition, these single chain Fab molecules might be further stabilized by generation of interchain disulfide bonds via insertion of cysteine residues (e.g. position 44 in the variable heavy chain and position 100 in the variable light chain according to Kabat numbering). The term “N-terminus denotes the last amino acid of the N-terminus. The term “C-terminus denotes the last amino acid of the C-terminus. 
     By “fused” or “connected” is meant that the components (e.g. a Fab molecule and an Fc domain subunit) are linked by peptide bonds, either directly or via one or more peptide linkers. 
     The term “linker” as used herein refers to a peptide linker and is preferably a peptide with an amino acid sequence with a length of at least 5 amino acids, preferably with a length of 5 to 100, more preferably of 10 to 50 amino acids. In one embodiment said peptide linker is (GxS)n or (GxS)nGm with G=glycine, S=serine, and (x=3, n=3, 4, 5 or 6, and m=0, 1, 2 or 3) or (x=4, n=2, 3, 4 or 5 and m=0, 1, 2 or 3), preferably x=4 and n=2 or 3, more preferably with x=4, n=2. In one embodiment said peptide linker is (G 4 S) 2 . 
     The term “immunoglobulin molecule” refers to a protein having the structure of a naturally occurring antibody. For example, immunoglobulins of the IgG class are heterotetrameric glycoproteins of about 150,000 daltons, composed of two light chains and two heavy chains that are disulfide-bonded. From N- to C-terminus, each heavy chain has a variable region (VH), also called a variable heavy domain or a heavy chain variable domain, followed by three constant domains (CH1, CH2, and CH3), also called a heavy chain constant region. Similarly, from N- to C-terminus, each light chain has a variable region (VL), also called a variable light domain or a light chain variable domain, followed by a constant light (CL) domain, also called a light chain constant region. The heavy chain of an immunoglobulin may be assigned to one of five types, called α (IgA), δ (IgD), ε (IgE), γ (IgG), or μ (IgM), some of which may be further divided into subtypes, e.g. γ 1  (IgG 1 ), γ 2  (IgG 2 ), γ 3  (IgG 3 ), γ 4  (IgG 4 ), α 1  (IgA 1 ) and α 2  (IgA 2 ). The light chain of an immunoglobulin may be assigned to one of two types, called kappa (κ) and lambda (λ), based on the amino acid sequence of its constant domain. An immunoglobulin essentially consists of two Fab molecules and an Fc domain, linked via the immunoglobulin hinge region. 
     An “antibody that binds to the same epitope” as a reference antibody refers to an antibody that blocks binding of the reference antibody to its antigen in a competition assay by 50% or more, and conversely, the reference antibody blocks binding of the antibody to its antigen in a competition assay by 50% or more. An exemplary competition assay is provided herein. 
     The term “antigen binding domain” refers to the part of an antigen binding molecule that comprises the area which specifically binds to and is complementary to part or all of an antigen. Where an antigen is large, an antigen binding molecule may only bind to a particular part of the antigen, which part is termed an epitope. An antigen binding domain may be provided by, for example, one or more antibody variable domains (also called antibody variable regions). Preferably, an antigen binding domain comprises an antibody light chain variable region (VL) and an antibody heavy chain variable region (VH). 
     The term “chimeric” antibody refers to an antibody in which a portion of the heavy and/or light chain is derived from a particular source or species, while the remainder of the heavy and/or light chain is derived from a different source or species, usually prepared by recombinant DNA techniques. Chimeric antibodies comprising a rabbit variable region and a human constant region are preferred. Other preferred forms of “chimeric antibodies” encompassed by the present invention are those in which the constant region has been modified or changed from that of the original antibody to generate the properties according to the invention, especially in regard to C1q binding and/or Fc receptor (FcR) binding. Such chimeric antibodies are also referred to as “class-switched antibodies”. Chimeric antibodies are the product of expressed immunoglobulin genes comprising DNA segments encoding immunoglobulin variable regions and DNA segments encoding immunoglobulin constant regions. Methods for producing chimeric antibodies involve conventional recombinant DNA and gene transfection techniques are well known in the art. See e.g. Morrison, S. L., et al., Proc. Natl. Acad. Sci. USA 81 (1984) 6851-6855; U.S. Pat. Nos. 5,202,238 and 5,204,244. 
     The term “cytotoxic agent” as used herein refers to a substance that inhibits or prevents a cellular function and/or causes cell death or destruction. Cytotoxic agents include, but are not limited to, radioactive isotopes (e.g., At 211 , I 131 , I 125 , Y 90 , Re 186 , Re 188 , Sm 153 , Bi 212 , P 32 , Pb 212  and radioactive isotopes of Lu); chemotherapeutic agents or drugs (e.g., methotrexate, adriamicin, vinca alkaloids (vincristine, vinblastine, etoposide), doxorubicin, melphalan, mitomycin C, chlorambucil, daunorubicin or other intercalating agents); growth inhibitory agents; enzymes and fragments thereof such as nucleolytic enzymes; antibiotics; toxins such as small molecule toxins or enzymatically active toxins of bacterial, fungal, plant or animal origin, including fragments and/or variants thereof; and the various antitumor or anticancer agents disclosed below. 
     “Effector functions” refer to those biological activities attributable to the Fc region of an antibody, which vary with the antibody isotype. Examples of antibody effector functions include: C1q binding and complement dependent cytotoxicity (CDC); Fc receptor binding; antibody-dependent cell-mediated cytotoxicity (ADCC); antibody-dependent cellular phagocytosis (ADCP), cytokine secretion, immune complex-mediated antigen uptake by antigen presenting cells; down regulation of cell surface receptors (e.g. B cell receptor); and B cell activation. 
     As used herein, the terms “engineer, engineered, engineering”, are considered to include any manipulation of the peptide backbone or the post-translational modifications of a naturally occurring or recombinant polypeptide or fragment thereof. Engineering includes modifications of the amino acid sequence, of the glycosylation pattern, or of the side chain group of individual amino acids, as well as combinations of these approaches. 
     The term “amino acid mutation” as used herein is meant to encompass amino acid substitutions, deletions, insertions, and modifications. Any combination of substitution, deletion, insertion, and modification can be made to arrive at the final construct, provided that the final construct possesses the desired characteristics, e.g., reduced binding to an Fc receptor, or increased association with another peptide. Amino acid sequence deletions and insertions include amino- and/or carboxy-terminal deletions and insertions of amino acids. Particular amino acid mutations are amino acid substitutions. For the purpose of altering e.g. the binding characteristics of an Fc region, non-conservative amino acid substitutions, i.e. replacing one amino acid with another amino acid having different structural and/or chemical properties, are particularly preferred. Amino acid substitutions include replacement by non-naturally occurring amino acids or by naturally occurring amino acid derivatives of the twenty standard amino acids (e.g. 4-hydroxyproline, 3-methylhistidine, ornithine, homoserine, 5-hydroxylysine). Amino acid mutations can be generated using genetic or chemical methods well known in the art. Genetic methods may include site-directed mutagenesis, PCR, gene synthesis and the like. It is contemplated that methods of altering the side chain group of an amino acid by methods other than genetic engineering, such as chemical modification, may also be useful. Various designations may be used herein to indicate the same amino acid mutation. For example, a substitution from proline at position 329 of the Fc domain to glycine can be indicated as 329G, G329, G 329 , P329G, or Pro329Gly. 
     An “effective amount” of an agent, e.g., a pharmaceutical formulation, refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired therapeutic or prophylactic result. 
     The term “Fc domain” or “Fc region” herein is used to define a C-terminal region of an immunoglobulin heavy chain that contains at least a portion of the constant region. The term includes native sequence Fc regions and variant Fc regions. Although the boundaries of the Fc region of an IgG heavy chain might vary slightly, the human IgG heavy chain Fc region is usually defined to extend from Cys226, or from Pro230, to the carboxyl-terminus of the heavy chain. However, the C-terminal lysine (Lys447) of the Fc region may or may not be present. Unless otherwise specified herein, numbering of amino acid residues in the Fc region or constant region is according to the EU numbering system, also called the EU index, as described in Kabat et al., Sequences of Proteins of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md., 1991. A “subunit” of an Fc domain as used herein refers to one of the two polypeptides forming the dimeric Fc domain, i.e. a polypeptide comprising C-terminal constant regions of an immunoglobulin heavy chain, capable of stable self-association. For example, a subunit of an IgG Fc domain comprises an IgG CH2 and an IgG CH3 constant domain. 
     A “modification promoting the association of the first and the second subunit of the Fc domain” is a manipulation of the peptide backbone or the post-translational modifications of an Fc domain subunit that reduces or prevents the association of a polypeptide comprising the Fc domain subunit with an identical polypeptide to form a homodimer. A modification promoting association as used herein particularly includes separate modifications made to each of the two Fc domain subunits desired to associate (i.e. the first and the second subunit of the Fc domain), wherein the modifications are complementary to each other so as to promote association of the two Fc domain subunits. For example, a modification promoting association may alter the structure or charge of one or both of the Fc domain subunits so as to make their association sterically or electrostatically favorable, respectively. Thus, (hetero)dimerization occurs between a polypeptide comprising the first Fc domain subunit and a polypeptide comprising the second Fc domain subunit, which might be non-identical in the sense that further components fused to each of the subunits (e.g. antigen binding moieties) are not the same. In some embodiments the modification promoting association comprises an amino acid mutation in the Fc domain, specifically an amino acid substitution. In a particular embodiment, the modification promoting association comprises a separate amino acid mutation, specifically an amino acid substitution, in each of the two subunits of the Fc domain. 
     “Framework” or “FR” refers to variable domain residues other than hypervariable region (HVR) residues. The FR of a variable domain generally consists of four FR domains: FR1, FR2, FR3, and FR4. Accordingly, the HVR and FR sequences generally appear in the following sequence in VH (or VL): FR1-H1(L1)-FR2-H2(L2)-FR3-H3(L3)-FR4. 
     The terms “full length antibody,” “intact antibody,” and “whole antibody” are used herein interchangeably to refer to an antibody having a structure substantially similar to a native antibody structure or having heavy chains that contain an Fc region as defined herein. 
     The terms “host cell,” “host cell line,” and “host cell culture” are used interchangeably and refer to cells into which exogenous nucleic acid has been introduced, including the progeny of such cells. Host cells include “transformants” and “transformed cells,” which include the primary transformed cell and progeny derived therefrom without regard to the number of passages. Progeny may not be completely identical in nucleic acid content to a parent cell, but may contain mutations. Mutant progeny that have the same function or biological activity as screened or selected for in the originally transformed cell are included herein. 
     A “human antibody” is one which possesses an amino acid sequence which corresponds to that of an antibody produced by a human or a human cell or derived from a non-human source that utilizes human antibody repertoires or other human antibody-encoding sequences. This definition of a human antibody specifically excludes a humanized antibody comprising non-human antigen-binding residues. As also mentioned for chimeric and humanized antibodies according to the invention the term “human antibody” as used herein also comprises such antibodies which are modified in the constant region to generate the properties according to the invention, especially in regard to C1q binding and/or FcR binding, e.g. by “class switching” i.e. change or mutation of Fc parts (e.g. from IgG1 to IgG4 and/or IgG1/IgG4 mutation.) 
     The term “recombinant human antibody”, as used herein, is intended to include all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as antibodies isolated from a host cell such as a NS0 or CHO cell or from an animal (e.g. a mouse) that is transgenic for human immunoglobulin genes or antibodies expressed using a recombinant expression vector transfected into a host cell. Such recombinant human antibodies have variable and constant regions in a rearranged form. The recombinant human antibodies according to the invention have been subjected to in vivo somatic hypermutation. Thus, the amino acid sequences of the VH and VL regions of the recombinant antibodies are sequences that, while derived from and related to human germ line VH and VL sequences, may not naturally exist within the human antibody germ line repertoire in vivo. 
     A “human consensus framework” is a framework which represents the most commonly occurring amino acid residues in a selection of human immunoglobulin VL or VH framework sequences. Generally, the selection of human immunoglobulin VL or VH sequences is from a subgroup of variable domain sequences. Generally, the subgroup of sequences is a subgroup as in Kabat et al.,  Sequences of Proteins of Immunological Interest , Fifth Edition, NIH Publication 91-3242, Bethesda Md. (1991), vols. 1-3. In one embodiment, for the VL, the subgroup is subgroup kappa I as in Kabat et al., supra. In one embodiment, for the VH, the subgroup is subgroup III as in Kabat et al., supra. 
     A “humanized” antibody refers to a chimeric antibody comprising amino acid residues from non-human HVRs and amino acid residues from human FRs. In certain embodiments, a humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the HVRs (e.g., CDRs) correspond to those of a non-human antibody, and all or substantially all of the FRs correspond to those of a human antibody. A humanized antibody optionally may comprise at least a portion of an antibody constant region derived from a human antibody. A “humanized form” of an antibody, e.g., a non-human antibody, refers to an antibody that has undergone humanization. Other forms of “humanized antibodies” encompassed by the present invention are those in which the constant region has been additionally modified or changed from that of the original antibody to generate the properties according to the invention, especially in regard to C1q binding and/or Fc receptor (FcR) binding. 
     The term “hypervariable region” or “HVR,” as used herein refers to each of the regions of an antibody variable domain which are hypervariable in sequence and/or form structurally defined loops (“hypervariable loops”). Generally, native four-chain antibodies comprise six HVRs; three in the VH (H1, H2, H3), and three in the VL (L1, L2, L3). HVRs generally comprise amino acid residues from the hypervariable loops and/or from the “complementarity determining regions” (CDRs), the latter being of highest sequence variability and/or involved in antigen recognition. Exemplary hypervariable loops occur at amino acid residues 26-32 (L1), 50-52 (L2), 91-96 (L3), 26-32 (H1), 53-55 (H2), and 96-101 (H3). (Chothia and Lesk,  J. Mol. Biol.  196:901-917 (1987).) Exemplary CDRs (CDR-L1, CDR-L2, CDR-L3, CDR-H1, CDR-H2, and CDR-H3) occur at amino acid residues 24-34 of L1, 50-56 of L2, 89-97 of L3, 31-35B of H1, 50-65 of H2, and 95-102 of H3. (Kabat et al.,  Sequences of Proteins of Immunological Interest,  5th Ed. Public Health Service, National Institutes of Health, Bethesda, Md. (1991).) Hypervariable regions (HVRs) are also referred to as complementarity determining regions (CDRs), and these terms are used herein interchangeably in reference to portions of the variable region that form the antigen binding regions. This particular region has been described by Kabat et al., U.S. Dept. of Health and Human Services, “Sequences of Proteins of Immunological Interest” (1983) and by Chothia et al.,  J. Mol. Biol.  196:901-917 (1987), where the definitions include overlapping or subsets of amino acid residues when compared against each other. Nevertheless, application of either definition to refer to a CDR of an antibody or variants thereof is intended to be within the scope of the term as defined and used herein. The appropriate amino acid residues which encompass the CDRs as defined by each of the above cited references are set forth below in Table A as a comparison. The exact residue numbers which encompass a particular CDR will vary depending on the sequence and size of the CDR. Those skilled in the art can routinely determine which residues comprise a particular CDR given the variable region amino acid sequence of the antibody. 
     
       
         
           
               
             
               
                 TABLE A 
               
             
            
               
                   
               
               
                 CDR Definitions 1   
               
            
           
           
               
               
               
               
               
            
               
                   
                 CDR 
                 Kabat 
                 Chothia 
                 AbM 2   
               
               
                   
                   
               
               
                   
                 V H  CDR1 
                 31-35 
                 26-32 
                 26-35 
               
               
                   
                 V H  CDR2 
                 50-65 
                 52-58 
                 50-58 
               
               
                   
                 V H  CDR3 
                  95-102 
                  95-102 
                  95-102 
               
               
                   
                 V L  CDR1 
                 24-34 
                 26-32 
                 24-34 
               
               
                   
                 V L  CDR2 
                 50-56 
                 50-52 
                 50-56 
               
               
                   
                 V L  CDR3 
                 89-97 
                 91-96 
                 89-97 
               
               
                   
                   
               
               
                   
                   1 Numbering of all CDR definitions in Table A is according to the numbering conventions set forth by Kabat et al. (see below). 
               
               
                   
                   2 “AbM” with a lowercase “b” as used in Table A refers to the CDRs as defined by Oxford Molecular’s “AbM” antibody modeling software. 
               
            
           
         
       
     
     Kabat et al. also defined a numbering system for variable region sequences that is applicable to any antibody. One of ordinary skill in the art can unambiguously assign this system of “Kabat numbering” to any variable region sequence, without reliance on any experimental data beyond the sequence itself. As used herein, “Kabat numbering” refers to the numbering system set forth by Kabat et al., U.S. Dept. of Health and Human Services, “Sequence of Proteins of Immunological Interest” (1983). Unless otherwise specified, references to the numbering of specific amino acid residue positions in an antibody variable region are according to the Kabat numbering system. 
     With the exception of CDR1 in VH, CDRs generally comprise the amino acid residues that form the hypervariable loops. CDRs also comprise “specificity determining residues,” or “SDRs,” which are residues that contact antigen. SDRs are contained within regions of the CDRs called abbreviated-CDRs, or a-CDRs. Exemplary a-CDRs (a-CDR-L1, a-CDR-L2, a-CDR-L3, a-CDR-H1, a-CDR-H2, and a-CDR-H3) occur at amino acid residues 31-34 of L1, 50-55 of L2, 89-96 of L3, 31-35B of H1, 50-58 of H2, and 95-102 of H3. (See Almagro and Fransson,  Front. Biosci.  13:1619-1633 (2008).) Unless otherwise indicated, HVR residues and other residues in the variable domain (e.g., FR residues) are numbered herein according to Kabat et al., supra. 
     An “immunoconjugate” is an antibody conjugated to one or more heterologous molecule(s), including but not limited to a cytotoxic agent. 
     An “individual” or “subject” is a mammal. Mammals include, but are not limited to, domesticated animals (e.g., cows, sheep, cats, dogs, and horses), primates (e.g., humans and non-human primates such as monkeys), rabbits, and rodents (e.g., mice and rats). In certain embodiments, the individual or subject is a human. 
     An “isolated” antibody is one which has been separated from a component of its natural environment. In some embodiments, an antibody is purified to greater than 95% or 99% purity as determined by, for example, electrophoretic (e.g., SDS-PAGE, isoelectric focusing (IEF), capillary electrophoresis) or chromatographic (e.g., ion exchange or reverse phase HPLC). For review of methods for assessment of antibody purity, see, e.g., Flatman et al.,  J. Chromatogr. B  848:79-87 (2007). 
     An “isolated” nucleic acid refers to a nucleic acid molecule that has been separated from a component of its natural environment. An isolated nucleic acid includes a nucleic acid molecule contained in cells that ordinarily contain the nucleic acid molecule, but the nucleic acid molecule is present extrachromosomally or at a chromosomal location that is different from its natural chromosomal location. 
     “Isolated nucleic acid encoding a bispecific antibody that specifically binds HER2” refers to one or more nucleic acid molecules encoding antibody heavy and light chains (or fragments thereof), including such nucleic acid molecule(s) in a single vector or separate vectors, and such nucleic acid molecule(s) present at one or more locations in a host cell. 
     The term “monoclonal antibody” as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical and/or bind the same epitope, except for possible variant antibodies, e.g., containing naturally occurring mutations or arising during production of a monoclonal antibody preparation, such variants generally being present in minor amounts. In contrast to polyclonal antibody preparations, which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody of a monoclonal antibody preparation is directed against a single determinant on an antigen. Thus, the modifier “monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method. For example, the monoclonal antibodies to be used in accordance with the present invention may be made by a variety of techniques, including but not limited to the hybridoma method, recombinant DNA methods, phage-display methods, and methods utilizing transgenic animals containing all or part of the human immunoglobulin loci, such methods and other exemplary methods for making monoclonal antibodies being described herein. 
     A “naked antibody” refers to an antibody that is not conjugated to a heterologous moiety (e.g., a cytotoxic moiety) or radiolabel. The naked antibody may be present in a pharmaceutical formulation. 
     “Native antibodies” refer to naturally occurring immunoglobulin molecules with varying structures. For example, native IgG antibodies are heterotetrameric glycoproteins of about 150,000 daltons, composed of two identical light chains and two identical heavy chains that are disulfide-bonded. From N- to C-terminus, each heavy chain has a variable region (VH), also called a variable heavy domain or a heavy chain variable domain, followed by three constant domains (CH1, CH2, and CH3). Similarly, from N- to C-terminus, each light chain has a variable region (VL), also called a variable light domain or a light chain variable domain, followed by a constant light (CL) domain. The light chain of an antibody may be assigned to one of two types, called kappa (κ) and lambda (λ), based on the amino acid sequence of its constant domain. 
     The term “package insert” is used to refer to instructions customarily included in commercial packages of therapeutic products, that contain information about the indications, usage, dosage, administration, combination therapy, contraindications and/or warnings concerning the use of such therapeutic products. 
     “No substantial cross-reactivity” means that a molecule (e.g., an antibody) does not recognize or specifically bind an antigen different from the actual target antigen of the molecule (e.g. an antigen closely related to the target antigen), particularly when compared to that target antigen. For example, an antibody may bind less than about 10% to less than about 5% to an antigen different from the actual target antigen, or may bind said antigen different from the actual target antigen at an amount consisting of less than about 10%, 9%, 8% 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, 0.2%, or 0.1%, preferably less than about 2%, 1%, or 0.5%, and most preferably less than about 0.2% or 0.1% antigen different from the actual target antigen. 
     “Percent (%) amino acid sequence identity” with respect to a reference polypeptide sequence is defined as the percentage of amino acid residues in a candidate sequence that are identical with the amino acid residues in the reference polypeptide sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, and not considering any conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways that are within the skill in the art, for instance, using publicly available computer software such as BLAST, BLAST-2, ALIGN or Megalign (DNASTAR) software. Those skilled in the art can determine appropriate parameters for aligning sequences, including any algorithms needed to achieve maximal alignment over the full length of the sequences being compared. For purposes herein, however, % amino acid sequence identity values are generated using the sequence comparison computer program ALIGN-2. The ALIGN-2 sequence comparison computer program was authored by Genentech, Inc., and the source code has been filed with user documentation in the U.S. Copyright Office, Washington D.C., 20559, where it is registered under U.S. Copyright Registration No. TXU510087. The ALIGN-2 program is publicly available from Genentech, Inc., South San Francisco, Calif., or may be compiled from the source code. The ALIGN-2 program should be compiled for use on a UNIX operating system, including digital UNIX V4.0D. All sequence comparison parameters are set by the ALIGN-2 program and do not vary. 
     In situations where ALIGN-2 is employed for amino acid sequence comparisons, the % amino acid sequence identity of a given amino acid sequence A to, with, or against a given amino acid sequence B (which can alternatively be phrased as a given amino acid sequence A that has or comprises a certain % amino acid sequence identity to, with, or against a given amino acid sequence B) is calculated as follows: 
       100 times the fraction X/Y 
     where X is the number of amino acid residues scored as identical matches by the sequence alignment program ALIGN-2 in that program&#39;s alignment of A and B, and where Y is the total number of amino acid residues in B. It will be appreciated that where the length of amino acid sequence A is not equal to the length of amino acid sequence B, the % amino acid sequence identity of A to B will not equal the % amino acid sequence identity of B to A. Unless specifically stated otherwise, all % amino acid sequence identity values used herein are obtained as described in the immediately preceding paragraph using the ALIGN-2 computer program. 
     The term “pharmaceutical formulation” refers to a preparation which is in such form as to permit the biological activity of an active ingredient contained therein to be effective, and which contains no additional components which are unacceptably toxic to a subject to which the formulation would be administered. 
     A “pharmaceutically acceptable carrier” refers to an ingredient in a pharmaceutical formulation, other than an active ingredient, which is nontoxic to a subject. A pharmaceutically acceptable carrier includes, but is not limited to, a buffer, excipient, stabilizer, or preservative. 
     As used herein, “treatment” (and grammatical variations thereof such as “treat” or “treating”) refers to clinical intervention in an attempt to alter the natural course of the individual being treated, and can be performed either for prophylaxis or during the course of clinical pathology. Desirable effects of treatment include, but are not limited to, preventing occurrence or recurrence of disease, alleviation of symptoms, diminishment of any direct or indirect pathological consequences of the disease, preventing metastasis, decreasing the rate of disease progression, amelioration or palliation of the disease state, and remission or improved prognosis. In some embodiments, antibodies of the invention are used to delay development of a disease or to slow the progression of a disease. 
     The term cancer as used herein refers to proliferative diseases, such as lymphomas, lymphocytic leukemias, lung cancer, non small cell lung (NSCL) cancer, bronchioloalviolar cell lung cancer, bone cancer, pancreatic cancer, skin cancer, cancer of the head or neck, cutaneous or intraocular melanoma, uterine cancer, ovarian cancer, rectal cancer, cancer of the anal region, stomach cancer, gastric cancer, colon cancer, breast cancer, uterine cancer, carcinoma of the fallopian tubes, carcinoma of the endometrium, carcinoma of the cervix, carcinoma of the vagina, carcinoma of the vulva, Hodgkin&#39;s Disease, cancer of the esophagus, cancer of the small intestine, cancer of the endocrine system, cancer of the thyroid gland, cancer of the parathyroid gland, cancer of the adrenal gland, sarcoma of soft tissue, cancer of the urethra, cancer of the penis, prostate cancer, cancer of the bladder, cancer of the kidney or ureter, renal cell carcinoma, carcinoma of the renal pelvis, mesothelioma, hepatocellular cancer, biliary cancer, neoplasms of the central nervous system (CNS), spinal axis tumors, brain stem glioma, glioblastoma multiforme, astrocytomas, schwanomas, ependymonas, medulloblastomas, meningiomas, squamous cell carcinomas, pituitary adenoma and Ewings sarcoma, including refractory versions of any of the above cancers, or a combination of one or more of the above cancers. 
     The term “variable region” or “variable domain” refers to the domain of an antibody heavy or light chain that is involved in binding the antibody to antigen. The variable domains of the heavy chain and light chain (VH and VL, respectively) of a native antibody generally have similar structures, with each domain comprising four conserved framework regions (FRs) and three hypervariable regions (HVRs). (See, e.g., Kindt et al.  Kuby Immunology,  6 th  ed., W.H. Freeman and Co., page 91 (2007).) A single VH or VL domain may be sufficient to confer antigen-binding specificity. Furthermore, antibodies that bind a particular antigen may be isolated using a VH or VL domain from an antibody that binds the antigen to screen a library of complementary VL or VH domains, respectively. See, e.g., Portolano et al.,  J. Immunol.  150:880-887 (1993); Clarkson et al.,  Nature  352:624-628 (1991). 
     The term “antigen-binding site of an antibody” when used herein refer to the amino acid residues of an antibody which are responsible for antigen-binding. The antigen-binding portion of an antibody comprises amino acid residues from the “complementary determining regions” or “CDRs”. “Framework” or “FR” regions are those variable domain regions other than the hypervariable region residues as herein defined. Therefore, the light and heavy chain variable domains of an antibody comprise from N- to C-terminus the domains FR1, CDR1, FR2, CDR2, FR3, CDR3, and FR4. Especially, CDR3 of the heavy chain is the region which contributes most to antigen binding and defines the antibody&#39;s properties. CDR and FR regions are determined according to the standard definition of Kabat et al., Sequences of Proteins of Immunological Interest, 5th ed., Public Health Service, National Institutes of Health, Bethesda, Md. (1991) and/or those residues from a “hypervariable loop”. 
     Antibody specificity refers to selective recognition of the antibody for a particular epitope of an antigen. Natural antibodies, for example, are monospecific. The term “monospecific” antibody as used herein denotes an antibody that has one or more binding sites each of which bind to the same epitope of the same antigen. 
     “Bispecific antibodies” according to the invention are antibodies which have two different antigen-binding specificities. Antibodies of the present invention are specific for two different epitopes of HER2, i.e. the extracellular domains II and IV of HER2. The term “bispecific” antibody as used herein denotes an antibody that has at least two binding sites each of which bind to different epitopes of the same antigen. 
     Bispecific antibodies may also be used to localize cytotoxic agents to cells which express HER2. Bispecific antibodies can be prepared as full length antibodies or antibody fragments. 
     The term “valent” as used within the current application denotes the presence of a specified number of binding sites in an antibody molecule. As such, the terms “bivalent”, “tetravalent”, and “hexavalent” denote the presence of two binding sites, four binding sites, and six binding sites, respectively, in an antibody molecule. The bispecific antibodies according to the invention are at least “bivalent” and may be “trivalent” or “multivalent” (e.g. “tetravalent” or “hexavalent”). 
     Antibodies of the present invention have two or more binding sites and are bispecific. That is, the antibodies may be bispecific even in cases where there are more than two binding sites (i.e. that the antibody is trivalent or multivalent). Bispecific antibodies of the invention include, for example, multivalent single chain antibodies, diabodies and triabodies, as well as antibodies having the constant domain structure of full length antibodies to which further antigen-binding sites (e.g., single chain Fv, a VH domain and/or a VL domain, Fab, or (Fab)2) are linked via one or more peptide-linkers. The antibodies can be full length from a single species, or be chimerized or humanized. 
     The term “vector,” as used herein, refers to a nucleic acid molecule capable of propagating another nucleic acid to which it is linked. The term includes the vector as a self-replicating nucleic acid structure as well as the vector incorporated into the genome of a host cell into which it has been introduced. Certain vectors are capable of directing the expression of nucleic acids to which they are operatively linked. Such vectors are referred to herein as “expression vectors.” 
     The term “amino acid” as used within this application denotes the group of naturally occurring carboxy α-amino acids comprising alanine (three letter code: ala, one letter code: A), arginine (arg, R), asparagine (asn, N), aspartic acid (asp, D), cysteine (cys, C), glutamine (gln, Q), glutamic acid (glu, E), glycine (gly, G), histidine (his, H), isoleucine (ile, I), leucine (leu, L), lysine (lys, K), methionine (met, M), phenylalanine (phe, F), proline (pro, P), serine (ser, S), threonine (thr, T), tryptophan (trp, W), tyrosine (tyr, Y), and valine (val, V). 
     As used herein, the expressions “cell”, “cell line”, and “cell culture” are used interchangeably and all such designations include progeny. Thus, the words “transfectants” and “transfected cells” include the primary subject cell and cultures derived there from without regard for the number of transfers. It is also understood that all progeny may not be precisely identical in DNA content, due to deliberate or inadvertent mutations. Variant progeny that have the same function or biological activity as screened for in the originally transformed cell are included. 
     “Affinity” refers to the strength of the sum total of noncovalent interactions between a single binding site of a molecule (e.g., an antibody) and its binding partner (e.g., an antigen). Unless indicated otherwise, as used herein, “binding affinity” refers to intrinsic binding affinity which reflects a 1:1 interaction between members of a binding pair (e.g., antibody and antigen). The affinity of a molecule X for its partner Y can generally be represented by the dissociation constant (Kd). Affinity can be measured by common methods known in the art, including those described herein. Specific illustrative and exemplary embodiments for measuring binding affinity are described in the following. 
     As used herein, the term “binding” or “specifically binding” refers to the binding of the antibody to an epitope of the antigen in an in-vitro assay, preferably in a surface plasmon resonance assay (SPR, BIAcore, GE-Healthcare Uppsala, Sweden). The affinity of the binding is defined by the terms ka (rate constant for the association of the antibody from the antibody/antigen complex), kD (dissociation constant), and KD (kD/ka). Binding or specifically binding means a binding affinity (KD) of 10-8 mol/l or less, preferably 10-9 M to 10-13 mol/l. 
     Binding of the antibody to the death receptor can be investigated by a BIAcore assay (GE-Healthcare Uppsala, Sweden). The affinity of the binding is defined by the terms ka (rate constant for the association of the antibody from the antibody/antigen complex), kD (dissociation constant), and KD (kD/ka) 
     The term “epitope” includes any polypeptide determinant capable of specific binding to an antibody. In certain embodiments, epitope determinant include chemically active surface groupings of molecules such as amino acids, sugar side chains, phosphoryl, or sulfonyl, and, in certain embodiments, may have specific three dimensional structural characteristics, and or specific charge characteristics. An epitope is a region of an antigen that is bound by an antibody. 
     As used herein, the terms “engineer, engineered, engineering,” particularly with the prefix “glyco-,” as well as the term “glycosylation engineering” are considered to include any manipulation of the glycosylation pattern of a naturally occurring or recombinant polypeptide or fragment thereof. Glycosylation engineering includes metabolic engineering of the glycosylation machinery of a cell, including genetic manipulations of the oligosaccharide synthesis pathways to achieve altered glycosylation of glycoproteins expressed in cells. Furthermore, glycosylation engineering includes the effects of mutations and cell environment on glycosylation. In one embodiment, the glycosylation engineering is an alteration in glycosyltransferase activity. In a particular embodiment, the engineering results in altered glucosaminyltransferase activity and/or fucosyltransferase activity. 
     II. Compositions and Methods 
     In one aspect, the invention is based on bispecific antibodies specifically binding to HER2. Antibodies of the invention are useful, e.g., for the treatment or diagnosis of cancer. 
     A. Exemplary Bispecific Antibodies Specifically Binding to HER2 with a Common Light Chain 
     In one aspect of the invention, a bispecific antibody specifically binding to HER2 is provided, wherein the antibody comprises a first binding moiety that specifically binds the extracellular domain II of HER2 and a second binding moiety that specifically binds the extracellular domain IV of HER2. The inventors of the present invention surprisingly generated a monovalent bispecific antibody wherein both binding moieties share a common light chain that retains the efficacy of the parent monospecific antibodies in terms of inhibition of tumor cell proliferation and has an increased affinity to the extracellular domain II of HER2. The generation of a bispecific molecule with a common light chain that retains the binding properties of both of the parent antibodies is not straight-forward as the common CDRs of the hybrid light chain have to retain the binding specificity for both the extracellular domains II and IV of HER2. The use of this so-called ‘common light chain’ principle, i.e. combining two binders that share one light chain but still have separate specificities, prevents light chain mispairing and in this particular case retains the epitope specificity of the parental antibodies. As a consequence, there are less side products during production, facilitating the homogenous preparation of HER2 bispecific antigen binding molecules at high yields. The heavy chain of Pertuzumab was further optimized, resulting in a more potent molecule in terms of affinity to the extracellular domain II of HER2. In addition, the Trastuzumab heavy chain has been stabilized by introducing certain mutations into the CDRs. The resulting molecule is superior to the parent Pertuzumab and Trastuzumab monospecific antibodies. The bispecific HER2 antibodies in the monovalent common light chain format have an increased affinity to the pertuzumab epitope, and show superior inhibitory effects on cell proliferation as compared to the combination of the parental antibodies. 
     In one embodiment a bispecific antibody is provided, comprising a first Fab molecule capable of specific binding to extracellular domain II of HER2 and a second Fab molecule capable of specific binding to extracellular domain IV of HER2, wherein the sequence of the variable light chain of the first Fab molecule is identical to the sequence of the variable light chain of the second Fab molecule (i.e. the first and the second Fab molecule comprise a common light chain). 
     In one embodiment, the invention provides a bispecific antibody that specifically binds to extracellular domains II and IV of HER2 comprising 
     a first heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 55;
 
(b) a heavy chain CDR2 of SEQ ID NO: 77;
 
(c) a heavy chain CDR3 of SEQ ID NO: 56;
 
     and a second heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 20;
 
(b) a heavy chain CDR2 of SEQ ID NO: 29;
 
(c) a heavy chain CDR3 of SEQ ID NO: 30;
 
     and a first and a second light chain comprising 
     (a) a light chain CDR1 of SEQ ID NO: 89;
 
(b) a light chain CDR2 of SEQ ID NO: 90;
 
(c) a light chain CDR3 of SEQ ID NO: 19.
 
     In one embodiment, the invention provides a bispecific antibody that specifically binds to extracellular domains II and IV of HER2 comprising 
     a first heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 14;
 
(b) a heavy chain CDR2 of SEQ ID NO: 60;
 
(c) a heavy chain CDR3 of SEQ ID NO: 16;
 
     and a second heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 20;
 
(b) a heavy chain CDR2 of SEQ ID NO: 29;
 
(c) a heavy chain CDR3 of SEQ ID NO: 30;
 
     and a first and a second light chain comprising 
     (a) a light chain CDR1 of SEQ ID NO: 89;
 
(b) a light chain CDR2 of SEQ ID NO: 90;
 
(c) a light chain CDR3 of SEQ ID NO: 19.
 
     In one embodiment, the invention provides a bispecific antibody that specifically binds to extracellular domains II and IV of HER2 comprising 
     a first heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 58;
 
(b) a heavy chain CDR2 of SEQ ID NO: 15;
 
(c) a heavy chain CDR3 of SEQ ID NO: 59;
 
     and a second heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 20;
 
(b) a heavy chain CDR2 of SEQ ID NO: 29;
 
(c) a heavy chain CDR3 of SEQ ID NO: 30;
 
     and a first and a second light chain comprising 
     (a) a light chain CDR1 of SEQ ID NO: 89;
 
(b) a light chain CDR2 of SEQ ID NO: 90;
 
(c) a light chain CDR3 of SEQ ID NO: 19.
 
     In one embodiment the second heavy chain of any of the embodiments above bears at least one modification in the amino acid sequence that confers higher chemical stability to the CDR, resulting in retained binding to HER2 under stress conditions. Modifications useful herein are e.g. D98E, D98N, D98T, G99A or G99S. Surprisingly the inventors found that some modifications of the CDRs did not only improve the stability of the molecule but also improved the binding affinity to HER2. 
     Hence in one embodiment, the invention provides a bispecific antibody that specifically binds to extracellular domains II and IV of HER2 comprising 
     a first heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 55;
 
(b) a heavy chain CDR2 of SEQ ID NO: 77;
 
(c) a heavy chain CDR3 of SEQ ID NO: 56;
 
     and a second heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 20;
 
(b) a heavy chain CDR2 of SEQ ID NO: 29;
 
(c) a heavy chain CDR3 of SEQ ID NO: 79;
 
     and a first and a second light chain comprising 
     (a) a light chain CDR1 of SEQ ID NO: 89;
 
(b) a light chain CDR2 of SEQ ID NO: 90;
 
(c) a light chain CDR3 of SEQ ID NO: 19.
 
     In one embodiment, the invention provides a bispecific antibody that specifically binds to extracellular domains II and IV of HER2 comprising 
     a first heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 14;
 
(b) a heavy chain CDR2 of SEQ ID NO: 60;
 
(c) a heavy chain CDR3 of SEQ ID NO: 16;
 
     and a second heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 20;
 
(b) a heavy chain CDR2 of SEQ ID NO: 29;
 
(c) a heavy chain CDR3 of SEQ ID NO: 79;
 
     and a first and a second light chain comprising 
     (a) a light chain CDR1 of SEQ ID NO: 89;
 
(b) a light chain CDR2 of SEQ ID NO: 90;
 
(c) a light chain CDR3 of SEQ ID NO: 19.
 
     In one embodiment, the invention provides a bispecific antibody that specifically binds to extracellular domains II and IV of HER2 comprising a first heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 58;
 
(b) a heavy chain CDR2 of SEQ ID NO: 15;
 
(c) a heavy chain CDR3 of SEQ ID NO: 59;
 
     and a second heavy chain comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 20;
 
(b) a heavy chain CDR2 of SEQ ID NO: 29;
 
(c) a heavy chain CDR3 of SEQ ID NO: 79;
 
     and a first and a second light chain comprising 
     (a) a light chain CDR1 of SEQ ID NO: 89;
 
(b) a light chain CDR2 of SEQ ID NO: 90;
 
(c) a light chain CDR3 of SEQ ID NO: 19.
 
     In one embodiment, the bispecific antibody comprises two variable light chains comprising an amino acid sequence of SEQ ID NO 54 (i.e. a common light chain), a first heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 64, and a second heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO 92. 
     In one embodiment, the bispecific antibody comprises two variable light chains comprising an amino acid sequence of SEQ ID NO 54 (i.e. a common light chain), a first heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 70, and a second heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO 92. 
     In one embodiment, the bispecific antibody comprises two variable light chains comprising an amino acid sequence of SEQ ID NO 54 (i.e. a common light chain), a first heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 68, and a second heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 92. 
     In one embodiment the second heavy chain of any of the embodiments above bears at least one modification in the amino acid sequence that confers stability to the CDRs and the binding to the target, e.g. D98E, D98N, D98T, G99A or G99S. 
     Hence in one embodiment, the bispecific antibody comprises two variable light chains comprising an amino acid sequence of SEQ ID NO: 54 (i.e. a common light chain), a first heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 64, and a second heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 117. 
     In one embodiment, the bispecific antibody comprises two variable light chains comprising an amino acid sequence of SEQ ID NO:54 (i.e. a common light chain), a first heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 70, and a second heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 117. 
     In one embodiment, the bispecific antibody comprises two variable light chains comprising an amino acid sequence of SEQ ID NO:54 (i.e. a common light chain), a first heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 68, and a second heavy chain comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 117. 
     In one embodiment, the bispecific antibody of the invention comprises a first heavy chain constant region comprising the amino acid sequence of SEQ ID NO: 114. 
     In one embodiment, the bispecific antibody of the invention comprises a second heavy chain constant region comprising the amino acid sequence of SEQ ID NO: 115. 
     In another embodiment the bispecific antibody of the invention comprises a first light chain constant region comprising the amino acid sequence of SEQ ID NO: 113. 
     In another embodiment the bispecific antibody of the invention comprises a second light chain constant region comprising the amino acid sequence of SEQ ID NO: 116. 
     In one embodiment a bispecific antibody is provided comprising SEQ ID NOs: 54, 113, 64, 114, 82, 116, 92 and 115. 
     In one embodiment a bispecific antibody is provided comprising SEQ ID NOs: 54, 113, 64, 114, 82, 116, 96 and 115. 
     In one embodiment the bispecific antibody of any of the above embodiments is glycoengineered, as outlined in section F below. In one embodiment the bispecific antibody of any of the above embodiments comprises a Fc domain modification that promotes heterodimerization as outlined in section D below. 
     B. Exemplary HER2 Bispecific Antibodies Comprising a Crossover Fab Fragment 
     In one embodiment of the invention, a bispecific antibody specifically binding to HER2 is provided, wherein the antibody comprises a first binding moiety that specifically binds to the extracellular domain II of HER2 and a second binding moiety that specifically binds to the extracellular domain IV of HER2. The inventors of the present invention generated a second monovalent bispecific antibody format wherein one of the binding moieties is a crossover Fab fragment. In one aspect of the invention a monovalent bispecific antibody is provided, wherein one of the Fab fragments of an IgG molecule is replaced by a crossover Fab fragment. Crossover Fab fragments are Fab fragments wherein either the variable regions or the constant regions of the heavy and light chain are exchanged. Bispecific antibody formats comprising crossover Fab fragments have been described, for example, in WO2009080252, WO2009080253, WO2009080251, WO2009080254, WO2010/136172, WO2010/145792 and WO2013/026831. The native Trastuzumab sequence has been optimized by introducing modifications into the CDRs of both the variable heavy chain and the variable light chain to improve stability and affinity, the resulting sequences framework-grafted to avoid mispairing of the light chains in the bispecific molecule, and the bispecific antibody glycoengineered, resulting in highly potent bispecific antibodies that target HER2 that can be produced with high yield and only low percentage of side products. In addition it shows superior inhibition of tumor cell proliferation as compared to the combination of the respective parental antibodies. 
     In one embodiment, the invention provides a bispecific antibody specifically binding to HER2 comprising 
     a first antigen binding site specific for extracellular domain II of HER2, comprising
 
(a) a heavy chain CDR1 of SEQ ID NO: 14;
 
(b) a heavy chain CDR2 of SEQ ID NO: 15;
 
(c) a heavy chain CDR3 of SEQ ID NO: 16;
 
(d) a light chain CDR1 of SEQ ID NO: 11;
 
(e) a light chain CDR2 of SEQ ID NO: 12;
 
(f) a light chain CDR3 of SEQ ID NO: 13;
 
     And a second antigen binding site specific for extracellular domain IV of HER2 comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 20;
 
(b) a heavy chain CDR2 of SEQ ID NO: 108;
 
(c) a heavy chain CDR3 of SEQ ID NO: 79;
 
(d) a light chain CDR1 of SEQ ID NO: 107;
 
(e) a light chain CDR2 of SEQ ID NO: 18;
 
(f) a light chain CDR3 of SEQ ID NO: 19;
 
     In one embodiment, the bispecific antibody comprises a first antigen binding site specific for the extracellular domain II of HER2 comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 22 and a variable light chain comprising an amino acid sequence of SEQ ID NO: 24; and a second antigen binding site specific for the extracellular domain IV of HER2, comprising a heavy chain variable region comprising an amino acid sequence of SEQ ID NO: 105 and a light chain variable region comprising an amino acid sequence of SEQ ID NO: 106. 
     In one embodiment, the invention provides a bispecific antibody that specifically binds to HER2 comprising 
     a first antigen binding site specific for extracellular domain II of HER2 comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 14; 
     (b) a heavy chain CDR2 of SEQ ID NO: 15; 
     (c) a heavy chain CDR3 of SEQ ID NO: 16; 
     (d) a light chain CDR1 of SEQ ID NO: 11; 
     (e) a light chain CDR2 of SEQ ID NO: 12; 
     (f) a light chain CDR3 of SEQ ID NO: 13. 
     and a second antigen binding site specific for extracellular domain IV of HER2, comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 20; 
     (b) a heavy chain CDR2 of SEQ ID NO: 29; 
     (c) a heavy chain CDR3 of SEQ ID NO: 79; 
     (d) a light chain CDR1 of SEQ ID NO: 104; 
     (e) a light chain CDR2 of SEQ ID NO: 18; 
     (f) a light chain CDR3 of SEQ ID NO: 19. 
     In one embodiment, the bispecific antibody comprises a first antigen binding site specific for the extracellular domain II of HER2 comprising a variable heavy chain comprising an amino acid sequence of SEQ ID NO: 22 and a variable light chain comprising an amino acid sequence of SEQ ID NO: 24; and a second antigen binding site specific for the extracellular domain IV of HER2, comprising a heavy chain variable region comprising an amino acid sequence of SEQ ID NO: 117 and a light chain variable region comprising an amino acid sequence of SEQ ID NO: 118. 
     In one embodiment, the invention provides a bispecific antibody that specifically binds to HER2 comprising 
     a first antigen binding site specific for extracellular domain II of HER2 comprising 
     (a) a heavy chain CDR1 of SEQ ID NO: 14; 
     (b) a heavy chain CDR2 of SEQ ID NO: 15; 
     (c) a heavy chain CDR3 of SEQ ID NO: 16; 
     (d) a light chain CDR1 of SEQ ID NO: 11; 
     (e) a light chain CDR2 of SEQ ID NO: 12; 
     (f) a light chain CDR3 of SEQ ID NO: 13. 
     and a second antigen binding site specific for extracellular domain IV of HER2, comprising
 
(a) a heavy chain CDR1 of SEQ ID NO: 20;
 
(b) a heavy chain CDR2 of SEQ ID NO: 29;
 
(c) a heavy chain CDR3 selected from the group consisting of SEQ ID NO: 79, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 87, SEQ ID NO: 88;
 
(d) a light chain CDR1 selected from the group consisting of SEQ ID NO: 104, SEQ ID NO: 103 and SEQ ID NO: 158;
 
(e) a light chain CDR2 of SEQ ID NO: 18;
 
(f) a light chain CDR3 of SEQ ID NO: 19;
 
     In one embodiment, the bispecific antibody of the invention comprises a first heavy chain constant region comprising the amino acid sequence of SEQ ID NO: 114. 
     In one embodiment, the bispecific antibody of the invention comprises a first heavy chain constant region comprising the amino acid sequence of SEQ ID NO: 114, wherein the C-terminal Lysine has been removed. 
     In one embodiment, the bispecific antibody of the invention comprises a second heavy chain constant region comprising the amino acid sequence of SEQ ID NO: 115. 
     In one embodiment, the bispecific antibody of the invention comprises a second heavy chain constant region comprising the amino acid sequence of SEQ ID NO: 115, wherein the C-terminal Lysine has been removed. 
     In another embodiment the bispecific antibody of the invention comprises a first light chain constant region comprising the amino acid sequence of SEQ ID NO: 113. 
     In another embodiment the bispecific antibody of the invention comprises a second light chain constant region comprising the amino acid sequence of SEQ ID NO: 116. 
     In one embodiment a bispecific antibody is provided comprising SEQ ID NOs: 109, 110, 111 and 112. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises 
     an Fc domain, 
     one Fab fragment comprising a first antigen binding site specific for the extracellular domain II of HER2, 
     and one Fab fragment comprising a second antigen binding site specific for the extracellular domain IV of HER2, 
     wherein either the variable regions or the constant regions of the heavy and light chain of at least one Fab fragment are exchanged. 
     Since the above bispecific antibody is bivalent with one binding site for the extracellular domain II of HER2 and one binding site for the extracellular domain IV of HER2, this format is also referred to as “1+1” format. Hence the bispecific antibodies described in this section are monovalent for the extracellular domain II of HER2 and monovalent for the extracellular domain IV of HER2. An exemplary structure of a bispecific antibody with a 1+1 format is depicted in  FIGS. 10A and 10B . Due to the exchange of either the variable regions or the constant regions, the Fab fragment above is also referred to as “cross-Fab fragment” or “xFab fragment” or “crossover Fab fragment”. The IgG molecule in a 1+1 format is also referred to as Crossmab format (see Schaefer et al. Proc Natl Acad Sci USA 2011; 108:11187-92). 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises 
     an Fc domain, 
     one Fab fragment comprising an antigen binding site specific for the extracellular domain II of HER2, wherein either the variable regions or the constant regions of the heavy and light chain are exchanged. 
     and one Fab fragment comprising an antigen binding site specific for the extracellular domain IV of HER2. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises 
     an Fc domain, 
     one Fab fragment comprising an antigen binding site specific for the extracellular domain II of HER2, 
     and one Fab fragment comprising an antigen binding site specific for the extracellular domain IV of HER2, wherein either the variable regions or the constant regions of the heavy and light chain are exchanged. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises 
     an Fc domain, 
     one Fab fragment comprising an antigen binding site specific for the extracellular domain II of HER2, 
     and one Fab fragment comprising an antigen binding site specific for the extracellular domain IV of HER2, wherein the variable regions of the heavy and light chain of the Fab fragment are exchanged. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises 
     an Fc domain, 
     one Fab fragments comprising an antigen binding site specific for the extracellular domain II of HER2, 
     and one Fab fragment comprising an antigen binding site specific for the extracellular domain IV of HER2, wherein the constant regions of the heavy and light chain are exchanged. 
     In one embodiment said bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises an Fc domain to which two Fab fragments are fused to the N-terminus, wherein either the variable regions or the constant regions of the heavy and light chain of at least one Fab fragment are exchanged. In one embodiment the two Fab fragments are fused to the N-terminus of the Fc domain through an immunoglobulin hinge region. In one embodiment, the immunoglobulin hinge region is a human IgG1 hinge region. In one embodiment the Fab fragment comprising an antigen binding site specific for the extracellular domain II of HER2, the Fab fragment comprising an antigen binding site specific for the extracellular domain IV of HER2 and the Fc domain are part of an immunoglobulin molecule. In a particular embodiment the immunoglobulin molecule is an IgG class immunoglobulin. In an even more particular embodiment the immunoglobulin is an IgG1 subclass immunoglobulin. In another embodiment the immunoglobulin is an IgG4 subclass immunoglobulin. In a further particular embodiment the immunoglobulin is a human immunoglobulin. In other embodiments the immunoglobulin is a chimeric immunoglobulin or a humanized immunoglobulin. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises an Immunoglobulin G (IgG) molecule with one binding site specific for the extracellular domain II of HER2 and one binding site specific for the extracellular domain IV of HER2, wherein either the variable regions or the constant regions of the heavy and light chain of one arm (Fab fragment) of the IgG molecule are exchanged. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises an Immunoglobulin G (IgG) molecule with one binding site specific for the extracellular domain II of HER2 and one binding site specific for the extracellular domain IV of HER2, wherein the variable regions of the heavy and light chain of one arm (Fab fragment) of the IgG molecule are exchanged. This antibody format is also referred to as CrossMab (VHVL) . 
     In one embodiment the variable regions of the heavy and light chain of the one arm (Fab fragment) of the IgG molecule which comprises the binding site specific for the extracellular domain IV of HER2 are exchanged. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises an Immunoglobulin G (IgG) molecule with one binding site specific for the extracellular domain II of HER2 and one binding site specific for the extracellular domain IV of HER2, wherein the constant regions of the heavy and light chain of one arm (Fab fragment) of the IgG molecule are exchanged. This antibody format is also referred to as CrossMab (CH1CL) . 
     In one embodiment the constant regions of the heavy and light chain of the one arm (Fab fragment) of the IgG molecule which comprises the binding site specific for the extracellular domain IV of HER2 are exchanged. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises an Immunoglobulin G (IgG) molecule with one binding site specific for the extracellular domain II of HER2 and one binding site specific for the extracellular domain IV of HER2, wherein the complete VH-CH1 and VL-CL domains of one arm (Fab fragment) of the IgG molecule are exchanged. This means that at least one of the Fab fragments is fused to the N-terminus of the Fc domain via the light chain (VLCL). In one embodiment the other Fab fragment is fused to the the N-terminus of the Fc domain via the heavy chain (VHCH1). 
     This antibody format is also referred to as CrossMab Fab . In one embodiment both Fab fragments are are fused to the N-terminus of the Fc domain through an immunoglobulin hinge region. 
     In one embodiment the bispecific antibody of any of the above embodiments is glycoengineered, as outlined in section F below. In one embodiment the bispecific antibody of any of the above embodiments comprises a Fc domain modification that promotes heterodimerization as outlined in section D below. 
     C. Fc Domain Modifications Promoting Heterodimerization 
     The bispecific HER2 antibodies of the invention comprise different antigen binding moieties, fused to one or the other of the two subunits of the Fc domain, thus the two subunits of the Fc domain are typically comprised in two non-identical polypeptide chains. Recombinant co-expression of these polypeptides and subsequent dimerization leads to several possible combinations of the two polypeptides. To improve the yield and purity of the bispecific antibodies of the invention in recombinant production, it will thus be advantageous to introduce in the Fc domain of the bispecific antibodies of the invention a modification promoting the association of the desired polypeptides. 
     Accordingly, in particular embodiments the Fc domain of the bispecific antibodies of the invention comprises a modification promoting the association of the first and the second subunit of the Fc domain. The site of most extensive protein-protein interaction between the two subunits of a human IgG Fc domain is in the CH3 domain of the Fc domain. Thus, in one embodiment said modification is in the CH3 domain of the Fc domain. 
     In a specific embodiment said modification is a so-called “knob-into-hole” modification, comprising a “knob” modification in one of the two subunits of the Fc domain and a “hole” modification in the other one of the two subunits of the Fc domain. 
     The knob-into-hole technology is described e.g. in U.S. Pat. Nos. 5,731,168; 7,695,936; Ridgway et al., Prot Eng 9, 617-621 (1996) and Carter, J Immunol Meth 248, 7-15 (2001). Generally, the method involves introducing a protuberance (“knob”) at the interface of a first polypeptide and a corresponding cavity (“hole”) in the interface of a second polypeptide, such that the protuberance can be positioned in the cavity so as to promote heterodimer formation and hinder homodimer formation. Protuberances are constructed by replacing small amino acid side chains from the interface of the first polypeptide with larger side chains (e.g. tyrosine or tryptophan). Compensatory cavities of identical or similar size to the protuberances are created in the interface of the second polypeptide by replacing large amino acid side chains with smaller ones (e.g. alanine or threonine). 
     Accordingly, in a particular embodiment, in the CH3 domain of the first subunit of the Fc domain of the bispecific antibodies of the invention an amino acid residue is replaced with an amino acid residue having a larger side chain volume, thereby generating a protuberance within the CH3 domain of the first subunit which is positionable in a cavity within the CH3 domain of the second subunit, and in the CH3 domain of the second subunit of the Fc domain an amino acid residue is replaced with an amino acid residue having a smaller side chain volume, thereby generating a cavity within the CH3 domain of the second subunit within which the protuberance within the CH3 domain of the first subunit is positionable. 
     The protuberance and cavity can be made by altering the nucleic acid encoding the polypeptides, e.g. by site-specific mutagenesis, or by peptide synthesis. 
     In a specific embodiment, in the CH3 domain of the first subunit of the Fc domain the threonine residue at position 366 is replaced with a tryptophan residue (T366W), and in the CH3 domain of the second subunit of the Fc domain the tyrosine residue at position 407 is replaced with a valine residue (Y407V). In one embodiment, in the second subunit of the Fc domain additionally the threonine residue at position 366 is replaced with a serine residue (T366S) and the leucine residue at position 368 is replaced with an alanine residue (L368A). 
     In yet a further embodiment, in the first subunit of the Fc domain additionally the serine residue at position 354 is replaced with a cysteine residue (S354C), and in the second subunit of the Fc domain additionally the tyrosine residue at position 349 is replaced by a cysteine residue (Y349C). Introduction of these two cysteine residues results in formation of a disulfide bridge between the two subunits of the Fc domain, further stabilizing the dimer (Carter, J Immunol Methods 248, 7-15 (2001)). 
     In an alternative embodiment a modification promoting association of the first and the second subunit of the Fc domain comprises a modification mediating electrostatic steering effects, e.g. as described in PCT publication WO 2009/089004. Generally, this method involves replacement of one or more amino acid residues at the interface of the two Fc domain subunits by charged amino acid residues so that homodimer formation becomes electrostatically unfavorable but heterodimerization electrostatically favorable. 
     In one embodiment a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises an Immunoglobulin G (IgG) molecule comprising a first antigen binding site specific for extracellular domain II of HER2 and a second antigen binding site specific for extracellular domain IV of HER2, wherein the Fc part of the first heavy chain comprises a first dimerization module and the Fc part of the second heavy chain comprises a second dimerization module allowing a heterodimerization of the two heavy chains of the IgG molecule. 
     In a further preferred embodiment, the first dimerization module comprises knobs and the second dimerization module comprises holes according to the knobs into holes strategy (see Carter P.; Ridgway J. B. B.; Presta L. G.: Immunotechnology, Volume 2, Number 1, February 1996, pp. 73-73(1)). 
     D. Nucleic Acid Sequences, Vectors and Methods of 
     The invention further provides isolated polynucleotides encoding a bispecific antibody specifically binding to HER2 as described herein or a fragment thereof. The polynucleotides encoding bispecific antibodies of the invention may be expressed as a single polynucleotide that encodes the entire bispecific antigen binding molecule or as multiple (e.g., two or more) polynucleotides that are co-expressed. Polypeptides encoded by polynucleotides that are co-expressed may associate through, e.g., disulfide bonds or other means to form a functional bispecific antibody. For example, the light chain portion of a Fab fragment may be encoded by a separate polynucleotide from the portion of the bispecific antibody comprising the heavy chain portion of the Fab fragment, an Fc domain subunit and optionally (part of) another Fab fragment. When co-expressed, the heavy chain polypeptides will associate with the light chain polypeptides to form the Fab fragment. In another example, the portion of the bispecific antibody provided therein comprising one of the two Fc domain subunits and optionally (part of) one or more Fab fragments could be encoded by a separate polynucleotide from the portion of the bispecific antibody provided therein comprising the other of the two Fc domain subunits and optionally (part of) a Fab fragment. When co-expressed, the Fc domain subunits will associate to form the Fc domain. 
     In one embodiment, the present invention is directed to an isolated polynucleotide encoding a bispecific antibody of the invention or a fragment thereof, wherein the polynucleotide comprises a sequence that encodes a first variable heavy chain sequence as shown in SEQ ID NOs 63, 67 and 69. In one embodiment, the present invention is directed to an isolated polynucleotide encoding a bispecific antibody of the invention or a fragment thereof, wherein the polynucleotide comprises a sequence that encodes a second variable heavy chain sequence as shown in SEQ ID NOs 91 and 133. 
     In one embodiment, the present invention is directed to an isolated polynucleotide encoding a bispecific antibody of the invention or a fragment thereof, wherein the polynucleotide comprises a sequence that encodes a variable light chain sequence as shown in SEQ ID NO: 53. 
     In another embodiment, the present invention is directed to an isolated polynucleotide encoding a bispecific antibody or fragment thereof, wherein the polynucleotide comprises a sequence that encodes a polypeptide sequence as shown in SEQ ID NOs 83, 85, 91, 93, 95, 97, 99, 101, 63, 67, 69, 53, 21 and 23. 
     In another embodiment, the invention is directed to an isolated polynucleotide encoding a bispecific antibody of the invention or a fragment thereof, wherein the polynucleotide comprises a sequence that encodes a first variable heavy chain sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identical to an amino acid sequence in SEQ ID NOs 63, 67 and 69. 
     In another embodiment, the invention is directed to an isolated polynucleotide encoding a bispecific antibody of the invention or a fragment thereof, wherein the polynucleotide comprises a sequence that encodes a second variable heavy chain sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identical to an amino acid sequence in SEQ ID NOs 91 and 133. 
     In another embodiment, the invention is directed to an isolated polynucleotide encoding a bispecific antibody of the invention or a fragment thereof, wherein the polynucleotide comprises a sequence that encodes a variable light chain sequence that is at least about 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identical to an amino acid sequence in SEQ ID NO: 53. 
     In certain embodiments the polynucleotide or nucleic acid is DNA. In other embodiments, a polynucleotide of the present invention is RNA, for example, in the form of messenger RNA (mRNA). RNA of the present invention may be single stranded or double stranded. 
     In further objects the present invention relates to an expression vector comprising a nucleic acid sequence of the present invention and to a prokaryotic or eukaryotic host cell comprising a vector of the present invention. In addition a method of producing an antibody comprising culturing the host cell so that the antibody is produced is provided. 
     E. Fc Domain Modifications Reducing Fc Receptor Binding and/or Effector Function 
     In one aspect a bispecific antibody that specifically binds to HER2 according to any of the above embodiments comprises an Immunoglobulin G (IgG) molecule wherein the Fc part is modified. The modified Fc part has a reduced binding affinity for the Fcγ receptors compared to a wildtype Fc part. 
     The Fc domain of the bispecific antibodies of the invention consists of a pair of polypeptide chains comprising heavy chain domains of an immunoglobulin molecule. For example, the Fc domain of an immunoglobulin G (IgG) molecule is a dimer, each subunit of which comprises the CH2 and CH3 IgG heavy chain constant domains. The two subunits of the Fc domain are capable of stable association with each other. 
     In one embodiment according the invention the Fc domain of the bispecific antibodies of the invention is an IgG Fc domain. In a particular embodiment the Fc domain is an IgG 1  Fc domain. In another embodiment the Fc domain is an IgG 4  Fc domain. In a more specific embodiment, the Fc domain is an IgG 4  Fc domain comprising an amino acid substitution at position S228 (Kabat numbering), particularly the amino acid substitution S228P. In a more specific embodiment, the Fc domain is an IgG 4  Fc domain comprising amino acid substitutions L235E and S228P and P329G. This amino acid substitution reduces in vivo Fab arm exchange of IgG 4  antibodies (see Stubenrauch et al., Drug Metabolism and Disposition 38, 84-91 (2010)). In a further particular embodiment the Fc domain is human. 
     The Fc domain confers favorable pharmacokinetic properties to the bispecific antibodies of the invention, including a long serum half-life which contributes to good accumulation in the target tissue and a favorable tissue-blood distribution ratio. At the same time it may, however, lead to undesirable targeting of the bispecific antibodies of the invention to cells expressing Fc receptors rather than to the preferred antigen-bearing cells. Accordingly, in particular embodiments the Fc domain of the the bispecific antibodies of the invention exhibits reduced binding affinity to an Fc receptor and/or reduced effector function, as compared to a native IgG 1  Fc domain. In one such embodiment the Fc domain (or the bispecific antibodies of the invention comprising said Fc domain) exhibits less than 50%, preferably less than 20%, more preferably less than 10% and most preferably less than 5% of the binding affinity to an Fc receptor, as compared to a native IgG 1  Fc domain (or a bispecific antibodies of the invention comprising a native IgG 1  Fc domain), and/or less than 50%, preferably less than 20%, more preferably less than 10% and most preferably less than 5% of the effector function, as compared to a native IgG 1  Fc domain domain (or a bispecific antibodies of the invention comprising a native IgG 1  Fc domain). In one embodiment, the Fc domain (or the bispecific antibodies of the invention comprising said Fc domain) does not substantially bind to an Fc receptor and/or induce effector function. In a particular embodiment the Fc receptor is an Fcγ receptor. In one embodiment the Fc receptor is a human Fc receptor. In one embodiment the Fc receptor is an activating Fc receptor. In a specific embodiment the Fc receptor is an activating human Fcγ receptor, more specifically human FcγRIIIa, FcγRI or FcγRIIa, most specifically human FcγRIIIa. In one embodiment the Fc receptor is an inhibitory Fc receptor. In a specific embodiment the Fc receptor is an inhibitory human Fcγ receptor, more specifically human FcgRIIB. In one embodiment the effector function is one or more of CDC, ADCC, ADCP, and cytokine secretion. In a particular embodiment the effector function is ADCC. In one embodiment the Fc domain domain exhibits substantially similar binding affinity to neonatal Fc receptor (FcRn), as compared to a native IgG 1  Fc domain domain. Substantially similar binding to FcRn is achieved when the Fc domain (or the bispecific antibodies of the invention comprising said Fc domain) exhibits greater than about 70%, particularly greater than about 80%, more particularly greater than about 90% of the binding affinity of a native IgG 1  Fc domain (or the bispecific antibodies of the invention comprising a native IgG 1  Fc domain) to FcRn. 
     In certain embodiments the Fc domain is engineered to have reduced binding affinity to an Fc receptor and/or reduced effector function, as compared to a non-engineered Fc domain. In particular embodiments, the Fc domain of the bispecific antibodies of the invention comprises one or more amino acid mutation that reduces the binding affinity of the Fc domain to an Fc receptor and/or effector function. Typically, the same one or more amino acid mutation is present in each of the two subunits of the Fc domain. In one embodiment the amino acid mutation reduces the binding affinity of the Fc domain to an Fc receptor. In one embodiment the amino acid mutation reduces the binding affinity of the Fc domain to an Fc receptor by at least 2-fold, at least 5-fold, or at least 10-fold. In embodiments where there is more than one amino acid mutation that reduces the binding affinity of the Fc domain to the Fc receptor, the combination of these amino acid mutations may reduce the binding affinity of the Fc domain to an Fc receptor by at least 10-fold, at least 20-fold, or even at least 50-fold. In one embodiment the bispecific antibodies of the invention comprising an engineered Fc domain exhibits less than 20%, particularly less than 10%, more particularly less than 5% of the binding affinity to an Fc receptor as compared to a bispecific antibodies of the invention comprising a non-engineered Fc domain. In a particular embodiment the Fc receptor is an Fcγ receptor. In some embodiments the Fc receptor is a human Fc receptor. In one embodiment the Fc receptor is an inhibitory Fc receptor. In a specific embodiment the Fc receptor is an inhibitory human Fcγ receptor, more specifically human FcgRIIB. In some embodiments the Fc receptor is an activating Fc receptor. In a specific embodiment the Fc receptor is an activating human Fcγ receptor, more specifically human FcγRIIIa, FcγRI or FcγRIIa, most specifically human FcγRIIIa. Preferably, binding to each of these receptors is reduced. In some embodiments binding affinity to a complement component, specifically binding affinity to C1q, is also reduced. In one embodiment binding affinity to neonatal Fc receptor (FcRn) is not reduced. Substantially similar binding to FcRn, i.e. preservation of the binding affinity of the Fc domain to said receptor, is achieved when the Fc domain (or the bispecific antibodies of the invention comprising said Fc domain) exhibits greater than about 70% of the binding affinity of a non-engineered form of the Fc domain (or the bispecific antibodies of the invention comprising said non-engineered form of the Fc domain) to FcRn. The Fc domain, or the bispecific antibodies of the invention of the invention comprising said Fc domain, may exhibit greater than about 80% and even greater than about 90% of such affinity. In certain embodiments the Fc domain of the bispecific antibodies of the invention is engineered to have reduced effector function, as compared to a non-engineered Fc domain. The reduced effector function can include, but is not limited to, one or more of the following: reduced complement dependent cytotoxicity (CDC), reduced antibody-dependent cell-mediated cytotoxicity (ADCC), reduced antibody-dependent cellular phagocytosis (ADCP), reduced cytokine secretion, reduced immune complex-mediated antigen uptake by antigen-presenting cells, reduced binding to NK cells, reduced binding to macrophages, reduced binding to monocytes, reduced binding to polymorphonuclear cells, reduced direct signaling inducing apoptosis, reduced dendritic cell maturation, or reduced T cell priming. In one embodiment the reduced effector function is one or more of reduced CDC, reduced ADCC, reduced ADCP, and reduced cytokine secretion. In a particular embodiment the reduced effector function is reduced ADCC. In one embodiment the reduced ADCC is less than 20% of the ADCC induced by a non-engineered Fc domain (or a bispecific antibody of the invention comprising a non-engineered Fc domain). 
     In one embodiment the amino acid mutation that reduces the binding affinity of the Fc domain to an Fc receptor and/or effector function is an amino acid substitution. In one embodiment the Fc domain comprises an amino acid substitution at a position of E233, L234, L235, N297, P331 and P329. In a more specific embodiment the Fc domain comprises an amino acid substitution at a position of L234, L235 and P329. In some embodiments the Fc domain comprises the amino acid substitutions L234A and L235A. In one such embodiment, the Fc domain is an IgG 1  Fc domain, particularly a human IgG 1  Fc domain. In one embodiment the Fc domain comprises an amino acid substitution at position P329. In a more specific embodiment the amino acid substitution is P329A or P329G, particularly P329G. In one embodiment the Fc domain comprises an amino acid substitution at position P329 and a further amino acid substitution at a position selected from E233, L234, L235, N297 and P331. In a more specific embodiment the further amino acid substitution is E233P, L234A, L235A, L235E, N297A, N297D or P331S. In particular embodiments the Fc domain comprises amino acid substitutions at positions P329, L234 and L235. In more particular embodiments the Fc domain comprises the amino acid mutations L234A, L235A and P329G (“P329G LALA”). In one such embodiment, the Fc domain is an IgG 1  Fc domain, particularly a human IgG 1  Fc domain. The “P329G LALA” combination of amino acid substitutions almost completely abolishes Fcγ receptor binding of a human IgG 1  Fc domain, as described in PCT patent application no. PCT/EP2012/055393, incorporated herein by reference in its entirety. PCT/EP2012/055393 also describes methods of preparing such mutant Fc domains and methods for determining its properties such as Fc receptor binding or effector functions. 
     IgG 4  antibodies exhibit reduced binding affinity to Fc receptors and reduced effector functions as compared to IgG 1  antibodies. Hence, in some embodiments the Fc domain of the bispecific antibodies of the invention is an IgG 4  Fc domain, particularly a human IgG 4  Fc domain. In one embodiment the IgG 4  Fc domain comprises amino acid substitutions at position S228, specifically the amino acid substitution S228P. To further reduce its binding affinity to an Fc receptor and/or its effector function, in one embodiment the IgG 4  Fc domain comprises an amino acid substitution at position L235, specifically the amino acid substitution L235E. In another embodiment, the IgG 4  Fc domain comprises an amino acid substitution at position P329, specifically the amino acid substitution P329G. In a particular embodiment, the IgG 4  Fc domain comprises amino acid substitutions at positions S228, L235 and P329, specifically amino acid substitutions S228P, L235E and P329G. Such IgG 4  Fc domain mutants and their Fcγ receptor binding properties are described in PCT patent application no. PCT/EP2012/055393, incorporated herein by reference in its entirety. 
     In a particular embodiment the Fc domain exhibiting reduced binding affinity to an Fc receptor and/or reduced effector function, as compared to a native IgG 1  Fc domain, is a human IgG 1  Fc domain comprising the amino acid substitutions L234A, L235A and optionally P329G, or a human IgG 4  Fc domain comprising the amino acid substitutions S228P, L235E and optionally P329G. 
     In certain embodiments N-glycosylation of the Fc domain has been eliminated. In one such embodiment the Fc domain comprises an amino acid mutation at position N297, particularly an amino acid substitution replacing asparagine by alanine (N297A) or aspartic acid (N297D). 
     In addition to the Fc domains described hereinabove and in PCT patent application no. PCT/EP2012/055393, Fc domains with reduced Fc receptor binding and/or effector function also include those with substitution of one or more of Fc domain residues 238, 265, 269, 270, 297, 327 and 329 (U.S. Pat. No. 6,737,056). Such Fc mutants include Fc mutants with substitutions at two or more of amino acid positions 265, 269, 270, 297 and 327, including the so-called “DANA” Fc mutant with substitution of residues 265 and 297 to alanine (U.S. Pat. No. 7,332,581). 
     Mutant Fc domains can be prepared by amino acid deletion, substitution, insertion or modification using genetic or chemical methods well known in the art. Genetic methods may include site-specific mutagenesis of the encoding DNA sequence, PCR, gene synthesis, and the like. The correct nucleotide changes can be verified for example by sequencing. 
     Binding to Fc receptors can be easily determined e.g. by ELISA, or by Surface Plasmon Resonance (SPR) using standard instrumentation such as a BIAcore instrument (GE Healthcare), and Fc receptors such as may be obtained by recombinant expression. A suitable such binding assay is described herein. Alternatively, binding affinity of Fc domains or cell activating bispecific antigen binding molecules comprising an Fc domain for Fc receptors may be evaluated using cell lines known to express particular Fc receptors, such as human NK cells expressing FcγIIIa receptor. 
     Effector function of an Fc domain, or bispecific antibodies of the invention comprising an Fc domain, can be measured by methods known in the art. A suitable assay for measuring ADCC is described herein. Other examples of in vitro assays to assess ADCC activity of a molecule of interest are described in U.S. Pat. No. 5,500,362; Hellstrom et al. Proc Natl Acad Sci USA 83, 7059-7063 (1986) and Hellstrom et al., Proc Natl Acad Sci USA 82, 1499-1502 (1985); U.S. Pat. No. 5,821,337; Bruggemann et al., J Exp Med 166, 1351-1361 (1987). Alternatively, non-radioactive assays methods may be employed (see, for example, ACTI™ non-radioactive cytotoxicity assay for flow cytometry (CellTechnology, Inc. Mountain View, Calif.); and CytoTox 96® non-radioactive cytotoxicity assay (Promega, Madison, Wis.)). Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and Natural Killer (NK) cells. Alternatively, or additionally, ADCC activity of the molecule of interest may be assessed in vivo, e.g. in a animal model such as that disclosed in Clynes et al., Proc Natl Acad Sci USA 95, 652-656 (1998). 
     In some embodiments, binding of the Fc domain to a complement component, specifically to C1q, is reduced. Accordingly, in some embodiments wherein the Fc domain is engineered to have reduced effector function, said reduced effector function includes reduced CDC. C1q binding assays may be carried out to determine whether the bispecific antibodies of the invention is able to bind C1q and hence has CDC activity. See e.g., C1q and C3c binding ELISA in WO 2006/029879 and WO 2005/100402. To assess complement activation, a CDC assay may be performed (see, for example, Gazzano-Santoro et al., J Immunol Methods 202, 163 (1996); Cragg et al., Blood 101, 1045-1052 (2003); and Cragg and Glennie, Blood 103, 2738-2743 (2004)). 
     The following section describes preferred embodiments of the bispecific antibodies of the invention comprising Fc domain modifications reducing Fc receptor binding and/or effector function. 
     F. Antibody Variants 
     In certain embodiments, amino acid sequence variants of the bispecific antibodies provided herein are contemplated, in addition to those described above. For example, it may be desirable to improve the binding affinity and/or other biological properties of the bispecific antibody. Amino acid sequence variants of a bispecific antibody may be prepared by introducing appropriate modifications into the nucleotide sequence encoding the bispecific antibody, or by peptide synthesis. Such modifications include, for example, deletions from, and/or insertions into and/or substitutions of residues within the amino acid sequences of the antibody. Any combination of deletion, insertion, and substitution can be made to arrive at the final construct, provided that the final construct possesses the desired characteristics, e.g., antigen-binding. 
     1. Substitution, Insertion, and Deletion Variants 
     In certain embodiments, antibody variants having one or more amino acid substitutions are provided. Sites of interest for substitutional mutagenesis include the HVRs and FRs. Conservative substitutions are shown in Table B under the heading of “conservative substitutions.” More substantial changes are provided in Table B under the heading of “exemplary substitutions,” and as further described below in reference to amino acid side chain classes. Amino acid substitutions may be introduced into an antibody of interest and the products screened for a desired activity, e.g., retained/improved antigen binding, decreased immunogenicity, or improved ADCC or CDC. 
                             TABLE B               Original   Exemplary   Preferred       Residue   Substitutions   Substitutions                  Ala (A)   Val; Leu; Ile   Val       Arg (R)   Lys; Gln; Asn   Lys       Asn (N)   Gln; His; Asp, Lys; Arg   Gln       Asp (D)   Glu; Asn   Glu       Cys (C)   Ser; Ala   Ser       Gln (Q)   Asn; Glu   Asn       Glu (E)   Asp; Gln   Asp       Gly (G)   Ala   Ala       His (H)   Asn; Gln; Lys; Arg   Arg       Ile (I)   Leu; Val; Met; Ala; Phe; Norleucine   Leu       Leu (L)   Norleucine; Ile; Val; Met; Ala; Phe   Ile       Lys (K)   Arg; Gln; Asn   Arg       Met (M)   Leu; Phe; Ile   Leu       Phe (F)   Trp; Leu; Val; Ile; Ala; Tyr   Tyr       Pro (P)   Ala   Ala       Ser (S)   Thr   Thr       Thr (T)   Val; Ser   Ser       Trp (W)   Tyr; Phe   Tyr       Tyr (Y)   Trp; Phe; Thr; Ser   Phe       Val (V)   Ile; Leu; Met; Phe; Ala; Norleucine   Leu                    
Amino acids may be grouped according to common side-chain properties:
 
     (1) hydrophobic: Norleucine, Met, Ala, Val, Leu, Ile; 
     (2) neutral hydrophilic: Cys, Ser, Thr, Asn, Gln; 
     (3) acidic: Asp, Glu; 
     (4) basic: His, Lys, Arg; 
     (5) residues that influence chain orientation: Gly, Pro; 
     (6) aromatic: Trp, Tyr, Phe. 
     Non-conservative substitutions will entail exchanging a member of one of these classes for another class. 
     One type of substitutional variant involves substituting one or more hypervariable region residues of a parent antibody (e.g. a humanized or human antibody). Generally, the resulting variant(s) selected for further study will have modifications (e.g., improvements) in certain biological properties (e.g., increased affinity, reduced immunogenicity) relative to the parent antibody and/or will have substantially retained certain biological properties of the parent antibody. An exemplary substitutional variant is an affinity matured antibody, which may be conveniently generated, e.g., using phage display-based affinity maturation techniques such as those described herein. Briefly, one or more HVR residues are mutated and the variant antibodies displayed on phage and screened for a particular biological activity (e.g. binding affinity). 
     Alterations (e.g., substitutions) may be made in HVRs, e.g., to improve antibody affinity. Such alterations may be made in HVR “hotspots,” i.e., residues encoded by codons that undergo mutation at high frequency during the somatic maturation process (see, e.g., Chowdhury,  Methods Mol. Biol.  207:179-196 (2008)), and/or SDRs (a-CDRs), with the resulting variant VH or VL being tested for binding affinity. Affinity maturation by constructing and reselecting from secondary libraries has been described, e.g., in Hoogenboom et al. in  Methods in Molecular Biology  178:1-37 (O&#39;Brien et al., ed., Human Press, Totowa, N.J., (2001).) In some embodiments of affinity maturation, diversity is introduced into the variable genes chosen for maturation by any of a variety of methods (e.g., error-prone PCR, chain shuffling, or oligonucleotide-directed mutagenesis). A secondary library is then created. The library is then screened to identify any antibody variants with the desired affinity. Another method to introduce diversity involves HVR-directed approaches, in which several HVR residues (e.g., 4-6 residues at a time) are randomized. HVR residues involved in antigen binding may be specifically identified, e.g., using alanine scanning mutagenesis or modeling. CDR-H3 and CDR-L3 in particular are often targeted. 
     In certain embodiments, substitutions, insertions, or deletions may occur within one or more HVRs so long as such alterations do not substantially reduce the ability of the antibody to bind antigen. For example, conservative alterations (e.g., conservative substitutions as provided herein) that do not substantially reduce binding affinity may be made in HVRs. Such alterations may be outside of HVR “hotspots” or SDRs. In certain embodiments of the variant VH and VL sequences provided above, each HVR either is unaltered, or contains no more than one, two or three amino acid substitutions. 
     A useful method for identification of residues or regions of an antibody that may be targeted for mutagenesis is called “alanine scanning mutagenesis” as described by Cunningham and Wells (1989)  Science,  244:1081-1085. In this method, a residue or group of target residues (e.g., charged residues such as arg, asp, his, lys, and glu) are identified and replaced by a neutral or negatively charged amino acid (e.g., alanine or polyalanine) to determine whether the interaction of the antibody with antigen is affected. Further substitutions may be introduced at the amino acid locations demonstrating functional sensitivity to the initial substitutions. Alternatively, or additionally, a crystal structure of an antigen-antibody complex to identify contact points between the antibody and antigen. Such contact residues and neighboring residues may be targeted or eliminated as candidates for substitution. Variants may be screened to determine whether they contain the desired properties. 
     Amino acid sequence insertions include amino- and/or carboxyl-terminal fusions ranging in length from one residue to polypeptides containing a hundred or more residues, as well as intrasequence insertions of single or multiple amino acid residues. Examples of terminal insertions include an antibody with an N-terminal methionyl residue. Other insertional variants of the antibody molecule include the fusion to the N- or C-terminus of the antibody to an enzyme (e.g. for ADEPT) or a polypeptide which increases the serum half-life of the antibody. 
     2. Glycosylation Variants 
     In certain embodiments, a bispecific antibody provided herein is altered to increase or decrease the extent to which the antibody is glycosylated. Addition or deletion of glycosylation sites to an antibody may be conveniently accomplished by altering the amino acid sequence such that one or more glycosylation sites is created or removed. 
     Where the bispecific antibody comprises an Fc region, the carbohydrate attached thereto may be altered. Native antibodies produced by mammalian cells typically comprise a branched, biantennary oligosaccharide that is generally attached by an N-linkage to Asn297 of the CH2 domain of the Fc region. See, e.g., Wright et al.  TIBTECH  15:26-32 (1997). The oligosaccharide may include various carbohydrates, e.g., mannose, N-acetyl glucosamine (GlcNAc), galactose, and sialic acid, as well as a fucose attached to a GlcNAc in the “stem” of the biantennary oligosaccharide structure. In some embodiments, modifications of the oligosaccharide in a bispecific antibody of the invention may be made in order to create antibody variants with certain improved properties. 
     In one embodiment, bispecific antibody variants are provided having a carbohydrate structure that lacks fucose attached (directly or indirectly) to an Fc region. For example, the amount of fucose in such antibody may be from 1% to 80%, from 1% to 65%, from 5% to 65% or from 20% to 40%. The amount of fucose is determined by calculating the average amount of fucose within the sugar chain at Asn297, relative to the sum of all glycostructures attached to Asn 297 (e. g. complex, hybrid and high mannose structures) as measured by MALDI-TOF mass spectrometry, as described in WO 2008/077546, for example. Asn297 refers to the asparagine residue located at about position 297 in the Fc region (Eu numbering of Fc region residues); however, Asn297 may also be located about ±3 amino acids upstream or downstream of position 297, i.e., between positions 294 and 300, due to minor sequence variations in antibodies. Such fucosylation variants may have improved ADCC function. See, e.g., US Patent Publication Nos. US 2003/0157108 (Presta, L.); US 2004/0093621 (Kyowa Hakko Kogyo Co., Ltd). Examples of publications related to “defucosylated” or “fucose-deficient” antibody variants include: US 2003/0157108; WO 2000/61739; WO 2001/29246; US 2003/0115614; US 2002/0164328; US 2004/0093621; US 2004/0132140; US 2004/0110704; US 2004/0110282; US 2004/0109865; WO 2003/085119; WO 2003/084570; WO 2005/035586; WO 2005/035778; WO2005/053742; WO2002/031140; Okazaki et al.  J. Mol. Biol.  336:1239-1249 (2004); Yamane-Ohnuki et al.  Biotech. Bioeng.  87: 614 (2004). Examples of cell lines capable of producing defucosylated antibodies include Lec13 CHO cells deficient in protein fucosylation (Ripka et al.  Arch. Biochem. Biophys.  249:533-545 (1986); US Pat Appl No US 2003/0157108 A1, Presta, L; and WO 2004/056312 A1, Adams et al., especially at Example 11), and knockout cell lines, such as alpha-1,6-fucosyltransferase gene, FUT8, knockout CHO cells (see, e.g., Yamane-Ohnuki et al.  Biotech. Bioeng.  87: 614 (2004); Kanda, Y. et al.,  Biotechnol. Bioeng.,  94(4):680-688 (2006); and WO2003/085107). 
     Bispecific antibodies variants are further provided with bisected oligosaccharides, e.g., in which a biantennary oligosaccharide attached to the Fc region of the bispecific antibody is bisected by GlcNAc. Such bispecific antibody variants may have reduced fucosylation and/or improved ADCC function. Examples of such antibody variants are described, e.g., in WO 2003/011878 (Jean-Mairet et al.); U.S. Pat. No. 6,602,684 (Umana et al.); and US 2005/0123546 (Umana et al.). Antibody variants with at least one galactose residue in the oligosaccharide attached to the Fc region are also provided. Such antibody variants may have improved CDC function. Such antibody variants are described, e.g., in WO 1997/30087 (Patel et al.); WO 1998/58964 (Raju, S.); and WO 1999/22764 (Raju, S.). 
     3. Cysteine Engineered Antibody Variants 
     In certain embodiments, it may be desirable to create cysteine engineered bispecific antibodies, e.g., “thioMAbs,” in which one or more residues of a bispecific antibody are substituted with cysteine residues. In particular embodiments, the substituted residues occur at accessible sites of the bispecific antibody. By substituting those residues with cysteine, reactive thiol groups are thereby positioned at accessible sites of the antibody and may be used to conjugate the antibody to other moieties, such as drug moieties or linker-drug moieties, to create an immunoconjugate. In certain embodiments, any one or more of the following residues may be substituted with cysteine: V205 (Kabat numbering) of the light chain; A118 (EU numbering) of the heavy chain; and S400 (EU numbering) of the heavy chain Fc region. Cysteine engineered antibodies may be generated as described, e.g., in U.S. Pat. No. 7,521,541. 
     G. Recombinant Methods and Compositions 
     Bispecific antibodies of the invention may be obtained, for example, by solid-state peptide synthesis (e.g. Merrifield solid phase synthesis) or recombinant production. For recombinant production one or more polynucleotide encoding the bispecific antibodies (or fragments), e.g., as described above, is isolated and inserted into one or more vectors for further cloning and/or expression in a host cell. Such polynucleotide may be readily isolated and sequenced using conventional procedures. In one embodiment a vector, preferably an expression vector, comprising one or more of the polynucleotides of the invention is provided. Methods which are well known to those skilled in the art can be used to construct expression vectors containing the coding sequence of a bispecific antibody (fragment) along with appropriate transcriptional/translational control signals. These methods include in vitro recombinant DNA techniques, synthetic techniques and in vivo recombination/genetic recombination. See, for example, the techniques described in Maniatis et al., M OLECULAR  C LONING: A  L ABORATORY  M ANUAL , Cold Spring Harbor Laboratory, N.Y. (1989); and Ausubel et al., C URRENT  P ROTOCOLS IN  M OLECULAR  B IOLOGY , Greene Publishing Associates and Wiley Interscience, N.Y (1989). The expression vector can be part of a plasmid, virus, or may be a nucleic acid fragment. The expression vector includes an expression cassette into which the polynucleotide encoding the bispecific antibody (fragment) (i.e. the coding region) is cloned in operable association with a promoter and/or other transcription or translation control elements. As used herein, a “coding region” is a portion of nucleic acid which consists of codons translated into amino acids. Although a “stop codon” (TAG, TGA, or TAA) is not translated into an amino acid, it may be considered to be part of a coding region, if present, but any flanking sequences, for example promoters, ribosome binding sites, transcriptional terminators, introns, 5′ and 3′ untranslated regions, and the like, are not part of a coding region. Two or more coding regions can be present in a single polynucleotide construct, e.g. on a single vector, or in separate polynucleotide constructs, e.g. on separate (different) vectors. Furthermore, any vector may contain a single coding region, or may comprise two or more coding regions, e.g. a vector of the present invention may encode one or more polypeptides, which are post- or co-translationally separated into the final proteins via proteolytic cleavage. In addition, a vector, polynucleotide, or nucleic acid of the invention may encode heterologous coding regions, either fused or unfused to a polynucleotide encoding the bispecific antibody (fragment) of the invention, or variant or derivative thereof. Heterologous coding regions include without limitation specialized elements or motifs, such as a secretory signal peptide or a heterologous functional domain. An operable association is when a coding region for a gene product, e.g. a polypeptide, is associated with one or more regulatory sequences in such a way as to place expression of the gene product under the influence or control of the regulatory sequence(s). Two DNA fragments (such as a polypeptide coding region and a promoter associated therewith) are “operably associated” if induction of promoter function results in the transcription of mRNA encoding the desired gene product and if the nature of the linkage between the two DNA fragments does not interfere with the ability of the expression regulatory sequences to direct the expression of the gene product or interfere with the ability of the DNA template to be transcribed. Thus, a promoter region would be operably associated with a nucleic acid encoding a polypeptide if the promoter was capable of effecting transcription of that nucleic acid. The promoter may be a cell-specific promoter that directs substantial transcription of the DNA only in predetermined cells. Other transcription control elements, besides a promoter, for example enhancers, operators, repressors, and transcription termination signals, can be operably associated with the polynucleotide to direct cell-specific transcription. Suitable promoters and other transcription control regions are disclosed herein. A variety of transcription control regions are known to those skilled in the art. These include, without limitation, transcription control regions, which function in vertebrate cells, such as, but not limited to, promoter and enhancer segments from cytomegaloviruses (e.g. the immediate early promoter, in conjunction with intron-A), simian virus 40 (e.g. the early promoter), and retroviruses (such as, e.g. Rous sarcoma virus). Other transcription control regions include those derived from vertebrate genes such as actin, heat shock protein, bovine growth hormone and rabbit â-globin, as well as other sequences capable of controlling gene expression in eukaryotic cells. Additional suitable transcription control regions include tissue-specific promoters and enhancers as well as inducible promoters (e.g. promoters inducible tetracyclins). Similarly, a variety of translation control elements are known to those of ordinary skill in the art. These include, but are not limited to ribosome binding sites, translation initiation and termination codons, and elements derived from viral systems (particularly an internal ribosome entry site, or IRES, also referred to as a CITE sequence). The expression cassette may also include other features such as an origin of replication, and/or chromosome integration elements such as retroviral long terminal repeats (LTRs), or adeno-associated viral (AAV) inverted terminal repeats (ITRs). 
     Polynucleotide and nucleic acid coding regions of the present invention may be associated with additional coding regions which encode secretory or signal peptides, which direct the secretion of a polypeptide encoded by a polynucleotide of the present invention. For example, if secretion of the bispecific antibody is desired, DNA encoding a signal sequence may be placed upstream of the nucleic acid encoding a bispecific antibody of the invention or a fragment thereof. According to the signal hypothesis, proteins secreted by mammalian cells have a signal peptide or secretory leader sequence which is cleaved from the mature protein once export of the growing protein chain across the rough endoplasmic reticulum has been initiated. Those of ordinary skill in the art are aware that polypeptides secreted by vertebrate cells generally have a signal peptide fused to the N-terminus of the polypeptide, which is cleaved from the translated polypeptide to produce a secreted or “mature” form of the polypeptide. In certain embodiments, the native signal peptide, e.g. an immunoglobulin heavy chain or light chain signal peptide is used, or a functional derivative of that sequence that retains the ability to direct the secretion of the polypeptide that is operably associated with it. Alternatively, a heterologous mammalian signal peptide, or a functional derivative thereof, may be used. For example, the wild-type leader sequence may be substituted with the leader sequence of human tissue plasminogen activator (TPA) or mouse β-glucuronidase. 
     DNA encoding a short protein sequence that could be used to facilitate later purification (e.g. a histidine tag) or assist in labeling the bispecific antibody may be included within or at the ends of the bispecific antibody (fragment) encoding polynucleotide. 
     In a further embodiment, a host cell comprising one or more polynucleotides of the invention is provided. In certain embodiments a host cell comprising one or more vectors of the invention is provided. The polynucleotides and vectors may incorporate any of the features, singly or in combination, described herein in relation to polynucleotides and vectors, respectively. In one such embodiment a host cell comprises (e.g. has been transformed or transfected with) a vector comprising a polynucleotide that encodes (part of) a bispecific antibody of the invention. As used herein, the term “host cell” refers to any kind of cellular system which can be engineered to generate the bispecific antibodies of the invention or fragments thereof. Host cells suitable for replicating and for supporting expression of bispecific antibodies are well known in the art. Such cells may be transfected or transduced as appropriate with the particular expression vector and large quantities of vector containing cells can be grown for seeding large scale fermenters to obtain sufficient quantities of the bispecific antibody for clinical applications. Suitable host cells include prokaryotic microorganisms, such as  E. coli , or various eukaryotic cells, such as Chinese hamster ovary cells (CHO), insect cells, or the like. For example, polypeptides may be produced in bacteria in particular when glycosylation is not needed. After expression, the polypeptide may be isolated from the bacterial cell paste in a soluble fraction and can be further purified. In addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast are suitable cloning or expression hosts for polypeptide-encoding vectors, including fungi and yeast strains whose glycosylation pathways have been “humanized”, resulting in the production of a polypeptide with a partially or fully human glycosylation pattern. See Gerngross, Nat Biotech 22, 1409-1414 (2004), and Li et al., Nat Biotech 24, 210-215 (2006). Suitable host cells for the expression of (glycosylated) polypeptides are also derived from multicellular organisms (invertebrates and vertebrates). Examples of invertebrate cells include plant and insect cells. Numerous baculoviral strains have been identified which may be used in conjunction with insect cells, particularly for transfection of  Spodoptera frugiperda  cells. Plant cell cultures can also be utilized as hosts. See e.g. U.S. Pat. Nos. 5,959,177, 6,040,498, 6,420,548, 7,125,978, and 6,417,429 (describing PLANTIBODIES™ technology for producing antibodies in transgenic plants). Vertebrate cells may also be used as hosts. For example, mammalian cell lines that are adapted to grow in suspension may be useful. Other examples of useful mammalian host cell lines are monkey kidney CV1 line transformed by SV40 (COS-7); human embryonic kidney line (293 or 293T cells as described, e.g., in Graham et al., J Gen Virol 36, 59 (1977)), baby hamster kidney cells (BHK), mouse sertoli cells (TM4 cells as described, e.g., in Mather, Biol Reprod 23, 243-251 (1980)), monkey kidney cells (CV1), African green monkey kidney cells (VERO-76), human cervical carcinoma cells (HELA), canine kidney cells (MDCK), buffalo rat liver cells (BRL 3A), human lung cells (W138), human liver cells (Hep G2), mouse mammary tumor cells (MMT 060562), TRI cells (as described, e.g., in Mather et al., Annals N.Y. Acad Sci 383, 44-68 (1982)), MRC 5 cells, and FS4 cells. Other useful mammalian host cell lines include Chinese hamster ovary (CHO) cells, including dhfr −  CHO cells (Urlaub et al., Proc Natl Acad Sci USA 77, 4216 (1980)); and myeloma cell lines such as YO, NS0, P3X63 and Sp2/0. For a review of certain mammalian host cell lines suitable for protein production, see, e.g., Yazaki and Wu, Methods in Molecular Biology, Vol. 248 (B. K. C. Lo, ed., Humana Press, Totowa, N.J.), pp. 255-268 (2003). Host cells include cultured cells, e.g., mammalian cultured cells, yeast cells, insect cells, bacterial cells and plant cells, to name only a few, but also cells comprised within a transgenic animal, transgenic plant or cultured plant or animal tissue. In one embodiment, the host cell is a eukaryotic cell, preferably a mammalian cell, such as a Chinese Hamster Ovary (CHO) cell, a human embryonic kidney (HEK) cell or a lymphoid cell (e.g., Y0, NS0, Sp20 cell). 
     Standard technologies are known in the art to express foreign genes in these systems. Cells expressing a polypeptide comprising either the heavy or the light chain of an antigen binding domain such as an antibody, may be engineered so as to also express the other of the antibody chains such that the expressed product is an antibody that has both a heavy and a light chain. 
     In one embodiment, a method of producing a bispecific antibody according to the invention is provided, wherein the method comprises culturing a host cell comprising a polynucleotide encoding the bispecific antibody, as provided herein, under conditions suitable for expression of the bispecific antibody, and recovering the bispecific antibody from the host cell (or host cell culture medium). 
     The components of the bispecific antibody are genetically fused to each other. Bispecific antibodies can be designed such that its components are fused directly to each other or indirectly through a linker sequence. The composition and length of the linker may be determined in accordance with methods well known in the art and may be tested for efficacy. Examples of linker sequences between different components of bispecific antibodies are found in the sequences provided herein. Additional sequences may also be included to incorporate a cleavage site to separate the individual components of the fusion if desired, for example an endopeptidase recognition sequence. 
     In certain embodiments the Fab fragments forming part of the bispecific antibody comprise at least an antibody variable region capable of binding an antigenic determinant. Variable regions can form part of and be derived from naturally or non-naturally occurring antibodies and fragments thereof. Methods to produce polyclonal antibodies and monoclonal antibodies are well known in the art (see e.g. Harlow and Lane, “Antibodies, a laboratory manual”, Cold Spring Harbor Laboratory, 1988). Non-naturally occurring antibodies can be constructed using solid phase-peptide synthesis, can be produced recombinantly (e.g. as described in U.S. Pat. No. 4,186,567) or can be obtained, for example, by screening combinatorial libraries comprising variable heavy chains and variable light chains (see e.g. U.S. Pat. No. 5,969,108 to McCafferty). 
     Any animal species of antibody, antibody fragment, antigen binding domain or variable region can be used in the bispecific antibodies of the invention. Non-limiting antibodies, antibody fragments, antigen binding domains or variable regions useful in the present invention can be of murine, primate, or human origin. If the bispecific antibody is intended for human use, a chimeric form of antibody may be used wherein the constant regions of the antibody are from a human. A humanized or fully human form of the antibody can also be prepared in accordance with methods well known in the art (see e. g. U.S. Pat. No. 5,565,332 to Winter). Humanization may be achieved by various methods including, but not limited to (a) grafting the non-human (e.g., donor antibody) CDRs onto human (e.g. recipient antibody) framework and constant regions with or without retention of critical framework residues (e.g. those that are important for retaining good antigen binding affinity or antibody functions), (b) grafting only the non-human specificity-determining regions (SDRs or a-CDRs; the residues critical for the antibody-antigen interaction) onto human framework and constant regions, or (c) transplanting the entire non-human variable domains, but “cloaking” them with a human-like section by replacement of surface residues. Humanized antibodies and methods of making them are reviewed, e.g., in Almagro and Fransson, Front Biosci 13, 1619-1633 (2008), and are further described, e.g., in Riechmann et al., Nature 332, 323-329 (1988); Queen et al., Proc Natl Acad Sci USA 86, 10029-10033 (1989); U.S. Pat. Nos. 5,821,337, 7,527,791, 6,982,321, and 7,087,409; Jones et al., Nature 321, 522-525 (1986); Morrison et al., Proc Natl Acad Sci 81, 6851-6855 (1984); Morrison and Oi, Adv Immunol 44, 65-92 (1988); Verhoeyen et al., Science 239, 1534-1536 (1988); Padlan, Molec Immun 31(3), 169-217 (1994); Kashmiri et al., Methods 36, 25-34 (2005) (describing SDR (a-CDR) grafting); Padlan, Mol Immunol 28, 489-498 (1991) (describing “resurfacing”); Dall&#39;Acqua et al., Methods 36, 43-60 (2005) (describing “FR shuffling”); and Osbourn et al., Methods 36, 61-68 (2005) and Klimka et al., Br J Cancer 83, 252-260 (2000) (describing the “guided selection” approach to FR shuffling). Human antibodies and human variable regions can be produced using various techniques known in the art. Human antibodies are described generally in van Dijk and van de Winkel, Curr Opin Pharmacol 5, 368-74 (2001) and Lonberg, Curr Opin Immunol 20, 450-459 (2008). Human variable regions can form part of and be derived from human monoclonal antibodies made by the hybridoma method (see e.g. Monoclonal Antibody Production Techniques and Applications, pp. 51-63 (Marcel Dekker, Inc., New York, 1987)). Human antibodies and human variable regions may also be prepared by administering an immunogen to a transgenic animal that has been modified to produce intact human antibodies or intact antibodies with human variable regions in response to antigenic challenge (see e.g. Lonberg, Nat Biotech 23, 1117-1125 (2005). Human antibodies and human variable regions may also be generated by isolating Fv clone variable region sequences selected from human-derived phage display libraries (see e.g., Hoogenboom et al. in Methods in Molecular Biology 178, 1-37 (O&#39;Brien et al., ed., Human Press, Totowa, N.J., 2001); and McCafferty et al., Nature 348, 552-554; Clackson et al., Nature 352, 624-628 (1991)). Phage typically display antibody fragments, either as single-chain Fv (scFv) fragments or as Fab fragments. 
     In certain embodiments, the Fab fragments useful in the present invention are engineered to have enhanced binding affinity according to, for example, the methods disclosed in U.S. Pat. Appl. Publ. No. 2004/0132066, the entire contents of which are hereby incorporated by reference. The ability of the bispecific antibody of the invention to bind to a specific antigenic determinant can be measured either through an enzyme-linked immunosorbent assay (ELISA) or other techniques familiar to one of skill in the art, e.g. surface plasmon resonance technique (analyzed on a BIACORE T100 system) (Liljeblad, et al., Glyco J 17, 323-329 (2000)), and traditional binding assays (Heeley, Endocr Res 28, 217-229 (2002)). Competition assays may be used to identify an antibody, antibody fragment, antigen binding domain or variable domain that competes with a reference antibody for binding to a particular antigen. In certain embodiments, such a competing antibody binds to the same epitope (e.g. a linear or a conformational epitope) that is bound by the reference antibody. Detailed exemplary methods for mapping an epitope to which an antibody binds are provided in Morris (1996) “Epitope Mapping Protocols,” in Methods in Molecular Biology vol. 66 (Humana Press, Totowa, N.J.). In an exemplary competition assay, immobilized antigen is incubated in a solution comprising a first labeled antibody that binds to the antigen and a second unlabeled antibody that is being tested for its ability to compete with the first antibody for binding to the antigen. The second antibody may be present in a hybridoma supernatant. As a control, immobilized antigen is incubated in a solution comprising the first labeled antibody but not the second unlabeled antibody. After incubation under conditions permissive for binding of the first antibody to the antigen, excess unbound antibody is removed, and the amount of label associated with immobilized antigen is measured. If the amount of label associated with immobilized antigen is substantially reduced in the test sample relative to the control sample, then that indicates that the second antibody is competing with the first antibody for binding to the antigen. See Harlow and Lane (1988) Antibodies: A Laboratory Manual ch. 14 (Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.). 
     Bispecific antibodies prepared as described herein may be purified by art-known techniques such as high performance liquid chromatography, ion exchange chromatography, gel electrophoresis, affinity chromatography, size exclusion chromatography, and the like. The actual conditions used to purify a particular protein will depend, in part, on factors such as net charge, hydrophobicity, hydrophilicity etc., and will be apparent to those having skill in the art. For affinity chromatography purification an antibody, ligand, receptor or antigen can be used to which the bispecific antibody binds. For example, for affinity chromatography purification of bispecific antibodies of the invention, a matrix with protein A or protein G may be used. Sequential Protein A or G affinity chromatography and size exclusion chromatography can be used to isolate a bispecific antibody essentially as described in the Examples. The purity of the bispecific antibody can be determined by any of a variety of well known analytical methods including gel electrophoresis, high pressure liquid chromatography, and the like. 
     H. Assays 
     Bispecific antibodies that specifically bind to HER2 provided herein may be identified, screened for, or characterized for their physical/chemical properties and/or biological activities by various assays known in the art. 
     1. Affinity Assays 
     The affinity of the bispecific antibody specifically binding to HER2 can be determined in accordance with the methods set forth in the Examples by surface plasmon resonance (SPR), using standard instrumentation such as a BIAcore instrument (GE Healthcare), and receptors or target proteins such as may be obtained by recombinant expression. Alternatively, binding of bispecific antibody provided therein to HER2 may be evaluated using cell lines expressing the particular receptor or target antigen, for example by flow cytometry (FACS). A specific illustrative and exemplary embodiment for measuring binding affinity is described in the following and in the Examples below. 
     According to one embodiment, K D  is measured by surface plasmon resonance using a BIACORE® T100 machine (GE Healthcare) at 25° C. 
     To analyze the interaction between the Fc-portion and Fc receptors, His-tagged recombinant Fc-receptor is captured by an anti-Penta His antibody (Qiagen) immobilized on CM5 chips and the bispecific constructs are used as analytes. Briefly, carboxymethylated dextran biosensor chips (CM5, GE Healthcare) are activated with N-ethyl-N′-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) according to the supplier&#39;s instructions. Anti Penta-His antibody is diluted with 10 mM sodium acetate, pH 5.0, to 40 μg/ml before injection at a flow rate of 5 μl/min to achieve approximately 6500 response units (RU) of coupled protein. Following the injection of the ligand, 1 M ethanolamine is injected to block unreacted groups. Subsequently the Fc-receptor is captured for 60 s at 4 or 10 nM. For kinetic measurements, four-fold serial dilutions of the bispecific construct (range between 500 nM and 4000 nM) are injected in HBS-EP (GE Healthcare, 10 mM HEPES, 150 mM NaCl, 3 mM EDTA, 0.05% Surfactant P20, pH 7.4) at 25° C. at a flow rate of 30 μl/min for 120 s. 
     To determine the affinity to the target antigen, bispecific constructs are captured by an anti human Fab specific antibody (GE Healthcare) that is immobilized on an activated CM5-sensor chip surface as described for the anti Penta-His antibody. The final amount of coupled protein is is approximately 12000 RU. The bispecific constructs are captured for 90 s at 300 nM. The target antigens are passed through the flow cells for 180 s at a concentration range from 250 to 1000 nM with a flowrate of 30 μl/min. The dissociation is monitored for 180 s. 
     Bulk refractive index differences are corrected for by subtracting the response obtained on reference flow cell. The steady state response was used to derive the dissociation constant K D  by non-linear curve fitting of the Langmuir binding isotherm. Association rates (k on ) and dissociation rates (k off ) are calculated using a simple one-to-one Langmuir binding model (BIACORE® T100 Evaluation Software version 1.1.1) by simultaneously fitting the association and dissociation sensorgrams. The equilibrium dissociation constant (K D ) is calculated as the ratio k off /k on . See, e.g., Chen et al., J Mol Biol 293, 865-881 (1999). 
     2. Binding Assays and Other Assays 
     In one aspect, a bispecific antibody of the invention is tested for its antigen binding activity, e.g., by known methods such as ELISA, Western blot, etc. 
     In another aspect, competition assays may be used to identify an antibody that competes with a specific anti-HER2 for binding to HER2. In certain embodiments, such a competing antibody binds to the same epitope (e.g., a linear or a conformational epitope) that is bound by a specific anti-HER2 antibody. Detailed exemplary methods for mapping an epitope to which an antibody binds are provided in Morris (1996) “Epitope Mapping Protocols,” in  Methods in Molecular Biology  vol. 66 (Humana Press, Totowa, N.J.). Further methods are described in the example section. 
     3. Activity Assays 
     In one aspect, assays are provided for identifying bispecific antibodies that bind to HER2 thereof having biological activity. Biological activity may include, e.g., DNA fragmentation, induction of apoptosis and lysis of targeted cells. Antibodies having such biological activity in vivo and/or in vitro are also provided. In one embodiment said activity is induction of complement-dependent cytotoxicity (CDC). In one embodiment said bispecific antibodies induce CDC to a higher degree than pertuzumab or trastuzumab alone. In one embodiment said bispecific antibodies induce CDC to at least an about 10 times higher degree, an about 20 times higher degree or an about 30 times higher degree than pertuzumab or trastuzumab alone. In another embodiment said bispecific antibodies induce CDC to a higher degree than the combination of pertuzumab or trastuzumab. In another embodiment said bispecific antibodies induce CDC to about a 30%, 40%, 50% or 60%, or at least a 30% to 70%, or at least a 40% to 60% higher degree than the combination of pertuzumab or trastuzumab. The complement-dependent cytotoxicity (CDC) assay can be performed with non heat-treated serum or commercially available complement fractions (see e.g. Lazar, G. A. et al. Engineered antibody Fc variants with enhanced effector function. Proc. Natl Acad. Sci. USA 103, 4005-4010 (2006)). Target cell killing can be assessed by several cell viability reagents such as Alamar Blue (Lazar, G. A. et al. Engineered antibody Fc variants with enhanced effector function. Proc. Natl Acad. Sci. USA 103, 4005-4010 (2006), Idusogie, E. E. et al. Engineered antibodies with increased activity to recruit complement. J. Immunol. 166, 2571-2575 (2001)), CellTiter-Glo (see e.g. Zhao, X. et al. Targeting C-type lectin-like molecule-1 for antibody-mediated immunotherapy in acute myeloid leukemia. Haematologica 95, 71-78 (2009)), LDH release (see e.g. Konishi, E., Kitai, Y. &amp; Kondo, T. Utilization of complement-dependent cytotoxicity to measure low levels of antibodies: application to nonstructural protein 1 in a model of Japanese encephalitis virus. Clin. Vaccine Immunol. 15, 88-94 (2008) and the examples disclosed herein) or calcein-AM release. In some embodiments said degree of induction of CDC is determined by a LDH release assay or a complement assay measuring binding of complement protein C1q to the antibodies of the invention bound to a cellular antigen. The CDC induction of the bispecific antibody is then compared to the CDC induction of either Pertuzumab or Trastuzumab alone, or the combination of Pertuzumab and Trastuzumab, with all values for CDC induction being assayed in the same assay, with the same cell line and the same respective antibody concentration. If performed in a microtiterplate, the capability of the bispecific antibodies and the controls (either Pertuzumab or Trastuzumab alone, or the combination of Pertuzumab and Trastuzumab) to induce CDC are preferably measured in the same microtiterplate using the same assay. Exemplary assays are disclosed, e.g. in example 18 or 19. In one embodiment said induction of CDC is determined on cancer cells, e.g. breast cancer cells. 
     In certain embodiments, a bispecific antibody of the invention is tested for such biological activity. Assays for detecting cell lysis (e.g. by measurement of LDH release) or apoptosis (e.g. using the TUNEL assay) are well known in the art. Assays for measuring ADCC or CDC are also described in WO 2004/065540 (see Example 1 therein), the entire content of which is incorporated herein by reference. 
     I. Pharmaceutical Formulations 
     Pharmaceutical formulations of a bispecific antibody that specifically binds to HER2 as described herein are prepared by mixing such bispecific antibody having the desired degree of purity with one or more optional pharmaceutically acceptable carriers ( Remington&#39;s Pharmaceutical Sciences  16th edition, Osol, A. Ed. (1980)), in the form of lyophilized formulations or aqueous solutions. Pharmaceutically acceptable carriers are generally nontoxic to recipients at the dosages and concentrations employed, and include, but are not limited to: buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride; benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose or sorbitol; salt-forming counter-ions such as sodium; metal complexes (e.g. Zn-protein complexes); and/or non-ionic surfactants such as polyethylene glycol (PEG). Exemplary pharmaceutically acceptable carriers herein further include insterstitial drug dispersion agents such as soluble neutral-active hyaluronidase glycoproteins (sHASEGP), for example, human soluble PH-20 hyaluronidase glycoproteins, such as rHuPH20 (HYLENEX®, Baxter International, Inc.). Certain exemplary sHASEGPs and methods of use, including rHuPH20, are described in US Patent Publication Nos. 2005/0260186 and 2006/0104968. In one aspect, a sHASEGP is combined with one or more additional glycosaminoglycanases such as chondroitinases. 
     Exemplary lyophilized antibody formulations are described in U.S. Pat. No. 6,267,958. Aqueous antibody formulations include those described in U.S. Pat. No. 6,171,586 and WO2006/044908, the latter formulations including a histidine-acetate buffer. 
     The formulation herein may also contain more than one active ingredients as necessary for the particular indication being treated, preferably those with complementary activities that do not adversely affect each other. Such active ingredients are suitably present in combination in amounts that are effective for the purpose intended. 
     Active ingredients may be entrapped in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly-(methylmethacylate) microcapsules, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules) or in macroemulsions. Such techniques are disclosed in  Remington&#39;s Pharmaceutical Sciences  16th edition, Osol, A. Ed. (1980). 
     Sustained-release preparations may be prepared. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the antibody, which matrices are in the form of shaped articles, e.g. films, or microcapsules. 
     The formulations to be used for in vivo administration are generally sterile. Sterility may be readily accomplished, e.g., by filtration through sterile filtration membranes. 
     J. Therapeutic Methods and Compositions 
     Any of the bispecific antibodies that bind to HER2 provided herein may be used in therapeutic methods. 
     In one aspect, a bispecific antibody that specifically binds to HER2 for use as a medicament is provided. In further aspects, a bispecific antibody that specifically binds to HER2 for use in treating cancer is provided. In certain embodiments, a bispecific antibody that specifically binds to HER2 for use in a method of treatment is provided. In certain embodiments, the invention provides a bispecific antibody that specifically binds to HER2 for use in a method of treating an individual having cancer comprising administering to the individual an effective amount of the bispecific antibody that specifically binds to HER2. In one such embodiment, the method further comprises administering to the individual an effective amount of at least one additional therapeutic agent, e.g., as described below. An “individual” according to any of the above embodiments is preferably a human. 
     In a further aspect, the invention provides for the use of a bispecific antibody that specifically binds to HER2 in the manufacture or preparation of a medicament. In one embodiment, the medicament is for treatment of cancer. In a further embodiment, the medicament is for use in a method of treating cancer comprising administering to an individual having cancer an effective amount of the medicament. In one such embodiment, the method further comprises administering to the individual an effective amount of at least one additional therapeutic agent, e.g., as described below. An “individual” according to any of the above embodiments may be a human. 
     In a further aspect, the invention provides a method for treating cancer. In one embodiment, the method comprises administering to an individual having cancer an effective amount of a bispecific antibody that binds to HER2. In one such embodiment, the method further comprises administering to the individual an effective amount of at least one additional therapeutic agent, as described below. An “individual” according to any of the above embodiments may be a human. 
     In a further aspect, the invention provides pharmaceutical formulations comprising any of the bispecific antibodies that bind to HER2 provided herein, e.g., for use in any of the above therapeutic methods. In one embodiment, a pharmaceutical formulation comprises any of the bispecific antibodies that bind to HER2 provided herein and a pharmaceutically acceptable carrier. In another embodiment, a pharmaceutical formulation comprises any of the bispecific antibodies that bind to HER2 provided herein and at least one additional therapeutic agent, e.g., as described below. 
     A bispecific antibody of the invention can be administered by any suitable means, including parenteral, intrapulmonary, and intranasal, and, if desired for local treatment, intralesional administration. Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration. Dosing can be by any suitable route, e.g. by injections, such as intravenous or subcutaneous injections, depending in part on whether the administration is brief or chronic. Various dosing schedules including but not limited to single or multiple administrations over various time-points, bolus administration, and pulse infusion are contemplated herein. 
     Bispecific antibodies of the invention would be formulated, dosed, and administered in a fashion consistent with good medical practice. Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners. The bispecific antibody need not be, but is optionally formulated with one or more agents currently used to prevent or treat the disorder in question. The effective amount of such other agents depends on the amount of antibody present in the formulation, the type of disorder or treatment, and other factors discussed above. These are generally used in the same dosages and with administration routes as described herein, or about from 1 to 99% of the dosages described herein, or in any dosage and by any route that is empirically/clinically determined to be appropriate. 
     For the prevention or treatment of disease, the appropriate dosage of a bispecific antibody of the invention will depend on the type of disease to be treated, the type of antibody, the severity and course of the disease, whether the bispecific antibody is administered for preventive or therapeutic purposes, previous therapy, the patient&#39;s clinical history and response to the bispecific antibody, and the discretion of the attending physician. The bispecific antibody is suitably administered to the patient at one time or over a series of treatments. Depending on the type and severity of the disease, about 1 μg/kg to 15 mg/kg (e.g. 0.1 mg/kg-10 mg/kg) of the bispecific antibody can be an initial candidate dosage for administration to the patient, whether, for example, by one or more separate administrations, or by continuous infusion. One typical daily dosage might range from about 1 μg/kg to 100 mg/kg or more, depending on the factors mentioned above. For repeated administrations over several days or longer, depending on the condition, the treatment would generally be sustained until a desired suppression of disease symptoms occurs. One exemplary dosage of the bispecific antibody would be in the range from about 0.05 mg/kg to about 10 mg/kg. Thus, one or more doses of about 0.5 mg/kg, 2.0 mg/kg, 4.0 mg/kg or 10 mg/kg may be administered to the patient. Such doses may be administered intermittently, e.g. every week or every three weeks (e.g. such that the patient receives from about two to about twenty, or e.g. about six doses of the bispecific antibody). An initial higher loading dose, followed by one or more lower doses may be administered. However, other dosage regimens may be useful. The progress of this therapy is easily monitored by conventional techniques and assays. 
     It is understood that any of the above formulations or therapeutic methods may be carried out using an immunoconjugate of the invention in place of or in addition to a bispecific antibody that specifically binds to HER2 of the invention. 
     K. Articles of Manufacture 
     In another aspect of the invention, an article of manufacture containing materials useful for the treatment, prevention and/or diagnosis of the disorders described above is provided. The article of manufacture comprises a container and a label or package insert on or associated with the container. Suitable containers include, for example, bottles, vials, syringes, IV solution bags, etc. The containers may be formed from a variety of materials such as glass or plastic. The container holds a composition which is by itself or combined with another composition effective for treating, preventing and/or diagnosing the condition and may have a sterile access port (for example the container may be an intravenous solution bag or a vial having a stopper pierceable by a hypodermic injection needle). At least one active agent in the composition is a bispecific antibody of the invention. The label or package insert indicates that the composition is used for treating the condition of choice. Moreover, the article of manufacture may comprise (a) a first container with a composition contained therein, wherein the composition comprises a bispecific antibody of the invention; and (b) a second container with a composition contained therein, wherein the composition comprises a further cytotoxic or otherwise therapeutic agent. The article of manufacture in this embodiment of the invention may further comprise a package insert indicating that the compositions can be used to treat a particular condition. Alternatively, or additionally, the article of manufacture may further comprise a second (or third) container comprising a pharmaceutically-acceptable buffer, such as bacteriostatic water for injection (BWFI), phosphate-buffered saline, Ringer&#39;s solution and dextrose solution. It may further include other materials desirable from a commercial and user standpoint, including other buffers, diluents, filters, needles, and syringes. 
     It is understood that any of the above articles of manufacture may include an immunoconjugate of the invention in place of or in addition to a bispecific antibody that specifically binds to HER2 of the invention. 
     L. Immunoconjugates 
     The invention also provides immunoconjugates comprising an bispecific antibody that specifically binds to HER2 herein conjugated to one or more cytotoxic agents, such as chemotherapeutic agents or drugs, growth inhibitory agents, toxins (e.g., protein toxins, enzymatically active toxins of bacterial, fungal, plant, or animal origin, or fragments thereof), or radioactive isotopes. 
     In one embodiment, an immunoconjugate is an antibody-drug conjugate (ADC) in which an antibody is conjugated to one or more drugs, including but not limited to a maytansinoid (see U.S. Pat. Nos. 5,208,020, 5,416,064 and European Patent EP 0 425 235 B1); an auristatin such as monomethylauristatin drug moieties DE and DF (MMAE and MMAF) (see U.S. Pat. Nos. 5,635,483 and 5,780,588, and 7,498,298); a dolastatin; a calicheamicin or derivative thereof (see U.S. Pat. Nos. 5,712,374, 5,714,586, 5,739,116, 5,767,285, 5,770,701, 5,770,710, 5,773,001, and 5,877,296; Hinman et al.,  Cancer Res.  53:3336-3342 (1993); and Lode et al.,  Cancer Res.  58:2925-2928 (1998)); an anthracycline such as daunomycin or doxorubicin (see Kratz et al.,  Current Med Chem.  13:477-523 (2006); Jeffrey et al.,  Bioorganic  &amp;  Med. Chem. Letters  16:358-362 (2006); Torgov et al.,  Bioconj. Chem.  16:717-721 (2005); Nagy et al.,  Proc. Natl. Acad. Sci. USA  97:829-834 (2000); Dubowchik et al.,  Bioorg . &amp;  Med. Chem. Letters  12:1529-1532 (2002); King et al.,  J. Med. Chem.  45:4336-4343 (2002); and U.S. Pat. No. 6,630,579); methotrexate; vindesine; a taxane such as docetaxel, paclitaxel, larotaxel, tesetaxel, and ortataxel; a trichothecene; and CC1065. 
     In another embodiment, an immunoconjugate comprises an antibody as described herein conjugated to an enzymatically active toxin or fragment thereof, including but not limited to diphtheria A chain, nonbinding active fragments of diphtheria toxin, exotoxin A chain (from  Pseudomonas aeruginosa ), ricin A chain, abrin A chain, modeccin A chain, alpha-sarcin,  Aleurites fordii  proteins, dianthin proteins,  Phytolaca americana  proteins (PAPI, PAPII, and PAP-S),  Momordica charantia  inhibitor, curcin, crotin,  Sapaonaria officinalis  inhibitor, gelonin, mitogellin, restrictocin, phenomycin, enomycin, and the tricothecenes. 
     In another embodiment, an immunoconjugate comprises an antibody as described herein conjugated to a radioactive atom to form a radioconjugate. A variety of radioactive isotopes are available for the production of radioconjugates. Examples include At 211 , I 131 , I 125 , Y 90 , Re 186 , Re 188 , Sm 153 , Bi 212 , P 32 , Pb 212  and radioactive isotopes of Lu. When the radioconjugate is used for detection, it may comprise a radioactive atom for scintigraphic studies, for example tc99m or I123, or a spin label for nuclear magnetic resonance (NMR) imaging (also known as magnetic resonance imaging, mri), such as iodine-123 again, iodine-131, indium-111, fluorine-19, carbon-13, nitrogen-15, oxygen-17, gadolinium, manganese or iron. 
     Conjugates of an antibody and cytotoxic agent may be made using a variety of bifunctional protein coupling agents such as N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP), succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate HCl), active esters (such as disuccinimidyl suberate), aldehydes (such as glutaraldehyde), bis-azido compounds (such as bis (p-azidobenzoyl) hexanediamine), bis-diazonium derivatives (such as bis-(p-diazoniumbenzoyl)-ethylenediamine), diisocyanates (such as toluene 2,6-diisocyanate), and bis-active fluorine compounds (such as 1,5-difluoro-2,4-dinitrobenzene). For example, a ricin immunotoxin can be prepared as described in Vitetta et al.,  Science  238:1098 (1987). Carbon-14-labeled 1-isothiocyanatobenzyl-3-methyldiethylene triaminepentaacetic acid (MX-DTPA) is an exemplary chelating agent for conjugation of radionucleotide to the antibody. See WO94/11026. The linker may be a “cleavable linker” facilitating release of a cytotoxic drug in the cell. For example, an acid-labile linker, peptidase-sensitive linker, photolabile linker, dimethyl linker or disulfide-containing linker (Chari et al.,  Cancer Res.  52:127-131 (1992); U.S. Pat. No. 5,208,020) may be used. 
     The immunuoconjugates or ADCs herein expressly contemplate, but are not limited to such conjugates prepared with cross-linker reagents including, but not limited to, BMPS, EMCS, GMBS, HBVS, LC-SMCC, MBS, MPBH, SBAP, SIA, SIAB, SMCC, SMPB, SMPH, sulfo-EMCS, sulfo-GMBS, sulfo-KMUS, sulfo-MBS, sulfo-SIAB, sulfo-SMCC, and sulfo-SMPB, and SVSB (succinimidyl-(4-vinylsulfone)benzoate) which are commercially available (e.g., from Pierce Biotechnology, Inc., Rockford, Ill., U.S.A). 
     III. Examples 
     The following are examples of methods and compositions of the invention. It is understood that various other embodiments may be practiced, given the general description provided above. 
     Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, the descriptions and examples should not be construed as limiting the scope of the invention. The disclosures of all patent and scientific literature cited herein are expressly incorporated in their entirety by reference. 
     Example 1: Materials and Methods 
     Unless stated otherwise the following general methods have been applied: 
     Recombinant DNA Techniques 
     Standard methods were used to manipulate DNA as described in Sambrook, J. et al., Molecular cloning: A laboratory manual; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989. The molecular biological reagents were used according to the manufacturer&#39;s instructions. 
     DNA and Protein Sequence Analysis and Sequence Data Management 
     General information regarding the nucleotide sequences of human immunoglobulins light and heavy chains is given in: Kabat, E., A., et al., (1991) Sequences of Proteins of Immunological Interest, Fifth Ed., NIH Publication No 91-3242. Amino acids of antibody chains are numbered according to EU numbering (Edelman, G. M., et al., PNAS 63 (1969) 78-85; Kabat, E. A., et al., (1991) Sequences of Proteins of Immunological Interest, Fifth Ed., NIH Publication No 91-3242). The GCG&#39;s (Genetics Computer Group, Madison, Wis.) software package version 10.2 and Infomax&#39;s Vector NTI Advance suite version 8.0 was used for sequence creation, mapping, analysis, annotation and illustration. 
     DNA Sequencing 
     DNA sequences were determined by double strand sequencing performed at SequiServe (Vaterstetten, Germany) and Geneart AG (Regensburg, Germany). 
     Example 2: Generation of Trastuzumab and Pertuzumab Bispecific Antibodies in a 2+2 IgG-scFv format 
     Gene Synthesis 
     Desired gene segments were prepared by Geneart AG (Regensburg, Germany) from synthetic oligonucleotides and PCR products by automated gene synthesis. The gene segments which are flanked by singular restriction endonuclease cleavage sites were cloned into pGA18 (ampR) plasmids. The plasmid DNA was purified from transformed bacteria and concentration determined by UV spectroscopy. The DNA sequence of the subcloned gene fragments was confirmed by DNA sequencing. 
     Construction of the Expression Plasmids 
     The following expression vector was used for the construction of all heavy and light chain encoding expression plasmids. The vector is composed of the following elements:
         a hygromycin resistance gene as a selection marker,   an origin of replication, oriP, of Epstein-Barr virus (EBV),   an origin of replication from the vector pUC18 which allows replication of this plasmid in  E. coli      a beta-lactamase gene which confers ampicillin resistance in  E. coli,      the immediate early enhancer and promoter from the human cytomegalovirus (HCMV),   the human 1-immunoglobulin polyadenylation (“poly A”) signal sequence, and       

     The immunoglobulin genes comprising the heavy or light chain were prepared by gene synthesis and cloned into pGA18 (ampR) plasmids as described above. Variable heavy chain constructs were constructed by directional cloning using unique restriction sites. Variable light chain constructs were ordered as gene synthesis comprising VL and CL and constructed by directional cloning using unique restriction sites. The final expression vectors were transformed into  E. coli  cells, expression plasmid DNA was isolated (Miniprep) and subjected to restriction enzyme analysis and DNA sequencing. Correct clones were grown in 150 ml LB-Amp medium, again plasmid DNA was isolated (Maxiprep) and sequence integrity confirmed by DNA sequencing. 
     Transient Expression of Immunoglobulin Variants in HEK293 Cells 
     Recombinant immunoglobulin variants were expressed by transient transfection of human embryonic kidney 293-F cells using the FreeStyle™ 293 Expression System according to the manufacturer&#39;s instruction (Invitrogen, USA). For small scale test expressions 30 ml of 0.5×10 6  HEK293F cells/ml were seeded one day prior to transfection. The next day, plasmid DNA (1 μg DNA per ml culture volume) was mixed with 1.2 ml Opti-MEM® I Reduced Serum Medium (Invitrogen, Carlsbad, Calif., USA) followed by addition of 40 μl of 293Fectin™ Transfection Reagent (Invitrogen, Carlsbad, Calif., USA). The mixture was incubated for 15 min at room temperature and added drop wise to the cells. One day post-transfection each flask was fed with 300 μl L-Glutamine (200 mM, Sigma-Aldrich, Steinheim, Germany) and 600 μl feed7 containing L-asparagine, amino acids, trace elements, ammonium-Fe(III) citrate, ethanolamine, trace elements, D-glucose, FreeStyle medium without RPMI. Three days post-transfection cell concentration, viability and glucose concentration in the medium were determined using an automated cell viability analyzer (Vi-CELL™ XR, Beckman Coulter, Fullerton, Calif., USA) and a glucose meter (Accu-CHEK® Sensor comfort, Roche Diagnostics GmbH, Mannheim, Germany). In addition each flask was fed with 300 μl of L-glutamine, 300 μl non-essential amino acids solution (PAN™ Biotech, Aidenbach, Germany), 300 μl sodium pyruvate (100 mM, Gibco, Invitrogen), 1.2 ml feed7 and ad 5 g/L glucose (D-(+)-Glucose solution 45%, Sigma). Finally, six days post-transfection antibodies were harvested by centrifugation at 3500 rpm in a X3R Multifuge (Heraeus, Buckinghamshire, England) for 15 min at ambient temperature, the supernatant was sterile filtered through a Steriflip filter unit (0.22 mm Millipore Express PLUS PES membrane, Millipore, Bedford, Mass.) and stored at −20° C. until further use. Large scale transfections up to 5 L were scaled linearly. 
     Purification of Bispecific and Control Antibodies 
     Bispecific antibodies were purified from cell culture supernatants by affinity chromatography using Protein A-Sepharose™ (GE Healthcare, Sweden) and Superdex200 size exclusion chromatography. Briefly, sterile filtered cell culture supernatants were applied on a HiTrap ProteinA HP (5 ml) column equilibrated with PBS buffer (10 mM Na 2 HPO 4 , 1 mM KH 2 PO 4 , 137 mM NaCl and 2.7 mM KCl, pH 7.4). Unbound proteins were washed out with equilibration buffer. Antibody and antibody variants were eluted with 0.1 M citrate buffer, pH 2.8, and the protein containing fractions were neutralized with 0.1 ml 1 M Tris, pH 8.5. Eluted protein fractions were pooled, concentrated with an Amicon Ultra centrifugal filter device (MWCO: 30 K, Millipore) to a volume of 3 ml and loaded on a Superdex200 HiLoad 120 ml 16/60 gel filtration column (GE Healthcare, Sweden) equilibrated with 20 mM Histidin, 140 mM NaCl, pH 6.0. Fractions containing purified bispecific and control antibodies with less than 5% high molecular weight aggregates were pooled and stored as 1.0 mg/ml aliquots at −80° C. 
     Protein Quantification 
     Proteins were quantified by affinity chromatography using the automated Ultimate 3000 system (Dionex, Idstein, Germany) with a pre-packed Poros® A protein A column (Applied Biosystems, Foster City, Calif., USA). All samples were loaded in buffer A (0.2 M Na 2 HPO 4 .[2H 2 O], pH 7.4) and eluted in buffer B (0.1 M citric acid, 0.2 M NaCl, pH 2.5). In order to determine the protein concentration an extinction coefficient of 1.62 was used for all samples. 
     Analysis of Purified Proteins 
     The protein concentration of purified protein samples was determined by measuring the optical density (OD) at 280 nm, using the molar extinction coefficient calculated on the basis of the amino acid sequence. Purity and molecular weight of bispecific and control antibodies were analyzed by SDS-PAGE in the presence and absence of a reducing agent (5 mM 1,4-dithiotreitol) and staining with Coomassie brilliant blue. The NuPAGE® Pre-Cast gel system (Invitrogen, USA) was used according to the manufacturer&#39;s instruction (4-20% Tris-Glycine gels). The aggregate content of bispecific and control antibody samples was analyzed by high-performance SEC using a Superdex 200 analytical size-exclusion column (GE Healthcare, Sweden) in 200 mM KH 2 PO 4 , 250 mM KCl, pH 7.0 running buffer at 25° C. 25 μg protein were injected on the column at a flow rate of 0.5 ml/min and eluted isocratic over 50 minutes. Integrity of the amino acid backbone of reduced bispecific antibody light and heavy chains was verified by NanoElectrospray Q-TOF mass spectrometry after removal of N-glycans by enzymatic treatment with Peptide-N-Glycosidase F (Roche Molecular Biochemicals). 
     Analytical HPLC 
     Antibodies were analyzed using a Agilent HPLC 1100 (Agilent Technologies, Palo Alto, Calif., USA) with a TSK-GEL G3000SW gel filtration column (7.5 mm ID×30 cm, TosoHaas Corp., Montgomeryville, Pa., USA). 18 μl of the eluted proteins were loaded onto the column in Buffer A (0.05 M K 2 HPO 4 /KH 2 PO 4  in 300 mM NaCl, pH 7.5) and separated based on size. 
     Reducing and Non-Reducing SDS-PAGE 
     7 μl of the eluted proteins were mixed with 2× sample buffer (NuPAGE® LDS Sample buffer, Invitrogen, Carlsbad, Calif., USA) and another 7 μl were mixed with 2× sample buffer containing 10% reducing agent (NuPAGE® Sample Reducing Agent, Invitrogen, Carlsbad, Calif., USA). Samples were heated to 70° for 10 min and loaded onto a pre-cast NuPAGE® 4-12% BisTris Gel (Invitrogen, Carlsbad, Calif., USA). The gel was run for 45 min at 200V and 125 mA. Afterwards the gel was washed three times with Millipore water and stained with SimplyBlue™ SafeStain (Invitrogen, Carlsbad, Calif., USA). The gel was destained overnight in Millipore water. 
       FIGS. 1A-1D  depict schematically the different variants of Trastuzumab and Pertuzumab bispecific antibodies in a 2+2 IgG-scFv format. All bispecific antibodies are bivalent for each antigen binding site and bind two different paratopes in the ErbB2/HER2 receptor (antigen1=trastuzumab specificity; antigen2=pertuzumab specificity). All bispecific antibodies in a 2+2 IgG-scFv format described herein are non frame-work grafted, non-CDR optimized, not glycoengineered and do not bear any mutation in the Fc part. 
       FIGS. 2A, 2B to 4  show exemplary size-exclusion purification graphs, SDS-PAGE analysis and analytical HPLC of variants of Trastuzumab and Pertuzumab bispecific antibodies in a 2+2 IgG-scFv format. No data shown for TvAB17, TvAB13 and variants Herceptin-scFv_A to E; all variants of Trastuzumab and Pertuzumab bispecific antibodies in a 2+2 IgG-scFv format were produced with the same quality. 
     
       
         
           
               
             
               
                 TABLE 1a 
               
             
            
               
                   
               
               
                 Sequences of Trastuzumab and Pertuzumab bispecific antibodies in a 2 + 2 IgG-scFv 
               
               
                 format 
               
            
           
           
               
               
               
            
               
                 SEQ 
                   
                   
               
               
                 ID 
                   
                   
               
               
                 NO 
                 Name 
                 Sequence 
               
               
                   
               
            
           
           
               
            
               
                 TvAB12_2431_TrastuzumabHCseFvOmnitarg(HC_LC) 
               
            
           
           
               
               
               
            
               
                 123 
                 Light chain 
                 diqmtqspsslsasvgdrvtitcrasqdvntavawyqqkpgkapklliysasflysgvpsrfsgs 
               
               
                   
                 (kappa) 
                 rsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikrtvaapsvfifppsdeqlksgtasvv 
               
               
                   
                 [Trastuzum 
                 cllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyacevt 
               
               
                   
                 ab, 1016] 
                 hqglsspvtksfnrgec 
               
               
                   
               
               
                 124 
                 Heavy 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryadsv 
               
               
                   
                 chain 
                 kgrftisadtskntaylqmnslraedtavyycsrwggdgfyamdywgqgtlvtvssastkgps 
               
               
                   
                 [Trastuzum 
                 vfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvps 
               
               
                   
                 ab + scFv 
                 sslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlmis 
               
               
                   
                 Omnitarg, 
                 rtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsyltvlhqdwlng 
               
               
                   
                 RB40] 
                 keykckvsnkalpapiektiskakgqprepqvytlppsreemtknqvsltclvkgfypsdiave 
               
               
                   
                   
                 wesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslsl 
               
               
                   
                   
                 spgkggggsggggsggggsevqlvesggglvqpggslrlscaasgftftdytmdwvrqapgk 
               
               
                   
                   
                 clewvadvnpnsggsiynqrfkgrftlsvdrskntlylqmnslraedtavyycarnlgpsfyfdy 
               
               
                   
                   
                 wgqgtlvtvssggggsggggsggggsggggsdiqmtqspsslsasvgdrvtitckasqdvsig 
               
               
                   
                   
                 vawyqqkpgkapklliysasyrytgvpsrfsgsgsgtdftltisslqpedfatyycqqyyiypytf 
               
               
                   
                   
                 gcgtkveik 
               
               
                   
               
            
           
           
               
            
               
                 TvAB13 [TvAb13_1330scFvTrastuzumab(LC_HC)OmnitargLC_IntronA_cDNA] 
               
            
           
           
               
               
               
            
               
                 125 
                 Light chain 
                 diqmtqspsslsasvgdrvtitcrasqdvntavawyqqkpgkapklliysasflysgvpsrfsgs 
               
               
                   
                 [scFv 
                 rsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikggggsggggsggggsevqlvesg 
               
               
                   
                 Trastuzuma 
                 gglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryadsvkgrftisad 
               
               
                   
                 b +  
                 tskntaylqmnslraedtavyycsrwggdgfyamdywgqgtlvtvssggggsggggsgggg 
               
               
                   
                 Omnitarg, 
                 sdiqmtqspsslsasvgdrvtitckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsg 
               
               
                   
                 RB34] 
                 sgsgtdftltisslqpedfatyycqqyyiypytfgqgtkveikrtvaapsvfifppsdeqlksgtas 
               
               
                   
                   
                 vvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyace 
               
               
                   
                   
                 vthqglsspvtksfnrgec 
               
               
                   
               
               
                 126 
                 Heavy 
                 evqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkglewvadvnpnsggsiynqr 
               
               
                   
                 chain 
                 fkgrftlsvdrskntlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtvssastkgpsvfp 
               
               
                   
                 (Omnitarg, 
                 lapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvpssslg 
               
               
                   
                 RB33) 
                 tqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlmisrtpe 
               
               
                   
                   
                 vtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlngkey 
               
               
                   
                   
                 kckvsnkalpapiektiskakgqprepqvytlppsrdeltknqvsltclvkgfypsdiavewesn 
               
               
                   
                   
                 gqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspgk 
               
               
                   
               
            
           
           
               
            
               
                 TvAB16 [TVAb16_2330TrastuzumabLCseFvOmnitarg(LC_HC)] 
               
            
           
           
               
               
               
            
               
                 127 
                 Light chain 
                 diqmtqspsslsasvgdrvtitcrasqdvntavawyqqkpgkapklliysasflysgvpsrfsgs 
               
               
                   
                 [Trastuzum 
                 rsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikrtvaapsvfifppsdeqlksgtasvv 
               
               
                   
                 ab +  
                 cllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyacevt 
               
               
                   
                 scFvOmnita 
                 hqglsspvtksfnrgecggggsggggsggggsdiqmtqspsslsasvgdrvtitckasqdvsig 
               
               
                   
                 rg, RB35] 
                 vawyqqkpgkapklliysasyrytgvpsrfsgsgsgtdftltisslqpedfatyycqqyyiypytf 
               
               
                   
                   
                 gqgtkveikggggsggggsggggsevqlvesggglvqpggslrlscaasgftftdytmdwvrq 
               
               
                   
                   
                 apgkglewvadvnpnsggsiynqrfkgrftlsvdrskntlylqmnslraedtavyycarnlgps 
               
               
                   
                   
                 fyfdywgqgtlvtvss 
               
               
                   
               
               
                 128 
                 Heavy 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryadsv 
               
               
                   
                 chain 
                 kgrftisadtskntaylqmnslraedtavyycsrwggdgfyamdywgqgtlvtvssastkgps 
               
               
                   
                 [Trastuzum 
                 vfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvps 
               
               
                   
                 ab, 1036] 
                 sslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlmis 
               
               
                   
                   
                 rtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlng 
               
               
                   
                   
                 keykckvsnkalpapiektiskakgqprepqvytlppsrdeltknqvsltclvkgfypsdiave 
               
               
                   
                   
                 wesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslsl 
               
               
                   
                   
                 spgk 
               
               
                   
               
            
           
           
               
            
               
                 TvAB17 [TvAb17_2431_TrastuzumabHCscFvOmnitarg(LC_HC)] 
               
            
           
           
               
               
               
            
               
                 129 
                 Light chain 
                 diqmtqspsslsasvgdrvtitcrasqdvntavawyqqkpgkapklliysasflysgvpsrfsgs 
               
               
                   
                 (kappa) 
                 rsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikrtvaapsvfifppsdeqlksgtasvv 
               
               
                   
                 [Trastuzum 
                 cllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyacevt 
               
               
                   
                 ab, 1016] 
                 hqglsspvtksfnrgec 
               
               
                   
               
               
                 130 
                 Heavy 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryadsv 
               
               
                   
                 chain 
                 kgrftisadtskntaylqmnslraedtavyycsrwggdgfyamdywgqgtlvtvssastkgps 
               
               
                   
                 [Trastuzum 
                 vfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvps 
               
               
                   
                 ab +  
                 sslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlmis 
               
               
                   
                 scFvOmnita 
                 rtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlng 
               
               
                   
                 rg, RB43] 
                 keykckvsnkalpapiektiskakgqprepqvytlppsreemtknqvsltclvkgfypsdiave 
               
               
                   
                   
                 wesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslsl 
               
               
                   
                   
                 spgkggggsggggsggggsdiqmtqspsslsasvgdrvtitckasqdvsigvawyqqkpgka 
               
               
                   
                   
                 pklliysasyrytgvpsrfsgsgsgtdftltisslqpedfatyycqqyyiypytfgcgtkveikggg 
               
               
                   
                   
                 gsggggsggggsggggsevqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkcle 
               
               
                   
                   
                 wvadvnpnsggsiynqrfkgrftlsvdrskntlylqmnslraedtavyycarnlgpsfyfdywg 
               
               
                   
                   
                 qgtlvtvss 
               
               
                   
               
            
           
           
               
            
               
                 TvAB20 [TVAb20_4441TrastuzumabLCscFvOmnitarg(LC_HC)] 
               
            
           
           
               
               
               
            
               
                 131 
                 Light chain 
                 diqmtqspsslsasvgdrvtitcrasqdvntavawyqqkpgkapklliysasflysgvpsrfsgs 
               
               
                   
                 [Trastuzum 
                 rsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikrtvaapsvfifppsdeqlksgtasvv 
               
               
                   
                 ab + scFv 
                 cllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyacevt 
               
               
                   
                 Onrmitarg, 
                 hqglsspvtksfnrgecggggsggggsggggsggggsdiqmtqspsslsasvgdrvtitckasq 
               
               
                   
                 RB61] 
                 dvsigvawyqqkpgkapklliysasyrytgvpsrfsgsgsgtdftltisslqpedfatyycqqyyi 
               
               
                   
                   
                 ypytfgcgtkveikggggsggggsggggsggggsevqlvesggglvqpggslrlscaasgftft 
               
               
                   
                   
                 dytmdwvrqapgkclewvadvnpnsggsiynqrfkgrftlsvdrskntlylqmnslraedtav 
               
               
                   
                   
                 yycarnlgpsfyfdywgqgtlvtvss 
               
               
                   
               
               
                 132 
                 Heavy 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryadsv 
               
               
                   
                 chain 
                 kgrftisadtskntaylqmnslraedtavyycsrwggdgfyamdywgqgtlvtvssastkgps 
               
               
                   
                 [Trastuzum 
                 vfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvps 
               
               
                   
                 ab, 1036] 
                 sslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlmis 
               
               
                   
                   
                 rtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlng 
               
               
                   
                   
                 keykckvsnkalpapiektiskakgqprepqvytlppsrdeltknqvsltclvkgfypsdiave 
               
               
                   
                   
                 wesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslsl 
               
               
                   
                   
                 spgk 
               
               
                   
               
            
           
           
               
            
               
                 Herceptarg 2 + 2 OmniE 
               
            
           
           
               
               
               
            
               
                 145 
                 Heavy chain 
                 evqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkglewvadvnpnsggsiynqrfkgrftlsvdrskn 
               
               
                   
                 with scFv 
                 tlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtvssastkgpsvfplapsskstsggtaalgclvkdyfpepv 
               
               
                   
                 Trastuzumab 
                 tvswnsgaltsgvhtfpavlqssglyslssvvtvpssslgtqtyicnvnhkpsntkvdkkvepkscdkhtcppcpap 
               
               
                   
                 stabilized with 
                 ellggpsvflfppkpkdtlmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltv 
               
               
                   
                 disulphide 
                 lhqdwlngkeykckvsnkalpapiektiskakgqprepqvytlppsrdeltknqvsltclvkgfypsdiavewesn 
               
               
                   
                 bonding 
                 gqpennykttppvldsdgsfflyskltvdksnvqqgnvfscsvmhealhnhytqkslslspgkggggsggggsev 
               
               
                   
                   
                 qlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryadsvkgrftisadtskntayl 
               
               
                   
                   
                 qmnslraedtavyycstwggegfyamdywgcgtlvtvssggggsggggsggggsdiqmtqspsslsasvgdrvt 
               
               
                   
                   
                 itcrasqdvnvavawyqqkpgkcpklliysasflysgvpsrfsgsrsgtdftltisslqpeclfatyycqqhyttpptfgq 
               
               
                   
                   
                 gtkveik 
               
               
                   
               
               
                 146 
                 Pertuzumab 
                 diqmtqspsslsasvgdrvtitckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsgsgsgtdftltisslqp 
               
               
                   
                 light chain 
                 edfatyycqqyyiypytfgqgtkveikrtvaapsvfifppsdeqlksgtasvvcllnnfypreakvqwkvdnalqsg 
               
               
                   
                   
                 nsqesvteqdskdstyslsstltlskadyekhkvyacevthqglsspvtksfnrgec 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 1 b) 
               
             
            
               
                   
               
               
                 Variants of TvAB12_2431_TrastuzumabHCscFvOmnitarg(HC_LC) with 
               
               
                 a stabilizing disulphide bond in the scFv part to reduce aggregation levels 
               
            
           
           
               
               
               
            
               
                   
                 Name of molecule 
                 Disulphide position (VH-VL) 
               
               
                   
                   
               
               
                   
                 Trastuzumab_scFv_WT 
                 WT 
               
               
                   
                 Trastuzumab_scFv_A 
                  44-100 
               
               
                   
                 Trastuzumab_scFv_B 
                  45-98 
               
               
                   
                 Trastuzumab_scFv_C 
                 101-46 
               
               
                   
                 Trastuzumab_scFv_D 
                 103-44 
               
               
                   
                 Trastuzumab_scFv_E 
                 105-43 
               
               
                   
                   
               
            
           
         
       
     
     Example 3: Proliferation Inhibition Assay with Trastuzumab and Pertuzumab Bispecific Antibodies in a 2+2 IgG-scFv Format 
     Cell Lines 
     MDA-MB 175 VII cells were maintained in DMEM/F12 medium (Gibco) supplemented with 10% fetal calf serum and 2 mL L-glutamine. Propagation of cell lines followed standard cell culture protocols. 
     The ability of the bispecific antibodies to inhibit proliferation was assessed in the cell line MDA-MB-175 VII. MDA-MB-175 VII were cultured in DMEM/F12 medium (Gibco) supplemented with 10% fetal calf serum, 2 mM L-glutamine. Cells in the logarithmic growth phase were detached, counted and 2×10e4 cells were seeded in 100 μL medium per well of a 96-well cell culture plate. Cells were maintained overnight in the incubator and the following day 100 μL of the respective antibodies diluted in medium were added in form of a dilution series to the cells. After a total incubation time of 6 days cell growth was assessed in an Alamar Blue (Invitrogen) assay. The assay was performed as recommended by the manufacturer. 
     Table 2 shows the potency of selected bispecific antibodies in the proliferation assay. 
     
       
         
           
               
               
               
               
             
               
                   
                   
               
               
                   
                 Antibody 
                 SEQ ID NO: 
                 EC50 [nM] 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 Herceptarg WT 
                   
                 1.84 
               
               
                   
                 Herceptarg A 
                   
                 1.89 
               
               
                   
                 Herceptarg B 
                   
                 2.66 
               
               
                   
                 Herceptarg C 
                   
                 2.56 
               
               
                   
                 Herceptarg D 
                   
                 1.63 
               
               
                   
                 Herceptarg E 
                   
                 1.75 
               
               
                   
                 TvAb12 
                 123/124 
                 4.90 
               
               
                   
                 CrossMab 
                 119/120/ 
                 4.75 
               
               
                   
                   
                 121/122 
               
               
                   
                 Pertuzumab 
                   
                 2.11 
               
               
                   
                 Pertuzumab + Herceptin 
                   
                 1.70 
               
               
                   
                 Herceptin 
                   
                 2.92 
               
               
                   
                   
               
            
           
         
       
     
     Example 4: Herceptarg Stabilisation 
     The aspartate isomerization site at position 98 of the heavy chain and the asparagine deamidation site at position 30 of the light chain are stability hotspots of trastuzumab. Those two positions affect the stability and integrity of the antigen binding capacity of the antibody. This problem was overcome by introducing the lyophilized formulations using either sodium succinate or histidine buffer. In order to increase stability and storage half-life we intended to replace those known sources of instability by amino acids, or amino acid stretches that should have a higher intrinsic stability. We tested herefore the replacement of Asp98 by Glu in the heavy chain and the replacement of Asn30 by Ser, as well Thr31 by Val in the light chain. Those mutations were abbreviated D98E, N30S, and T31V. T31V does not directly influence the deamidation of N30, but it was assumed that the residue adjacent on the C-terminal side of an Asparagine would influence the stability properties of a polypeptide chain. 
     The antibody samples were incubated over a period of either 1, 2, or 3 months at 40° C. in one of the three buffers: 40 mM Histidin, 150 mM NaCl, pH5.0, 40 mM Histidin, 150 mM NaCl, pH6.0, or 40 mM Histidin, 150 mM NaCL, pH7.4. The protein concentration during this period was always 1 mg/ml. After the indicated time points, samples were taken and shock frozen in liquid nitrogen, and then kept at −80° C. until further analysis. This analysis was carried out on a ProteOn XPR36 instrument (BioRad). Approximately 700 RU of Her2, respectively, were immobilized on 2 channels of a GLM chip using amine coupling (vertical orientation). Trastuzumab variants were measured in duplicates at 6 different analyte concentrations (100, 50, 25, 12.5, 6.25, 0 nM) by injections in horizontal orientation at 100 μl/min. Association rate were recorded for 180 s, the dissociation rate for 600 s. Regeneration was performed by two pulses of 10 mM glycine pH 1.5 and 50 mM NaOH for 60 s at 150 μl/min (horizontal orientation). In total four different Trastuzumab variants were measured. In the first experiment (Tables 3 and 4), only the unmodified Trastuzumab and variant 602 (D98E of heavy chain and T31V of light chain) were tested. In the second experiment the unmodified Trastuzumab, variant 602 (D98E of heavy chain and T31V of light chain) and variants VH:D98E/VL: N30S VH:D98E/VL: N30T were tested (Table 5, 6, 7). 
     Results: 
     All variants show the same affinity towards recombinant Her2 antigen as the parental Trastuzumab molecule. After exposure to pH5 or pH6 at 40° C., Trastuzumab lost affinity by a factor ˜5, mainly driven by increasing the off-rate and keeping the on-rate unchanged. Variant 602 showed almost undistinguishable affinities before and after pH stress. Variant N30S had a higher affinity from the beginning compared to the parental Trastuzumab, which stayed approximately constant during the stress conditions. The variants D98E (VH) together with either N30S, N30T, or T31V (VL) were used in the further experiments. Results are shown in  FIGS. 5A and 5B and 6A-6C  and the tables below. 
     
       
         
           
               
             
               
                 TABLE 3 
               
               
                   
               
               
                 Kinetic affinity parameters of Trastuzumab variants as determined by SPR 
               
               
                 method (ProteOn instrument) after incubating the samples for 1, 2, or 
               
               
                 3 months at 40° in 40 mM Histidin, 150 mM NaCl, pH 5.0 buffer. Each 
               
               
                 measurement was done in duplicate and both experimental values are shown. 
               
               
                 Tested were the resynthesized Trastuzumab (wt) and compared to the D98E 
               
               
                 T31V variant in the heavy and light chain, respectively (named clone 602). 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 t0 
                 t1 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 pH 5 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 wt 
                 1 
                 2.4E+05 
                 1.1E−04 
                 4.3E−10 
                 3.3E+05 
                 1.6E−04 
                 5.0E−10 
               
               
                   
                 2 
                 2.4E+05 
                 4.4E−05 
                 1.8E−10 
                 2.8E+05 
                 1.9E−04 
                 7.0E−10 
               
               
                 602 
                 1 
                 2.6E+05 
                 1.2E−04 
                 4.6E−10 
                 2.7E+05 
                 2.2E−04 
                 8.0E−10 
               
               
                   
                 2 
                 2.3E+05 
                 1.3E−04 
                 5.4E−10 
                 2.8E+05 
                 1.4E−04 
                 5.2E−10 
               
               
                   
               
            
           
           
               
               
               
            
               
                   
                 t2 
                 t3 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 pH 5 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 wt 
                 1 
                 2.9E+05 
                 2.6E−04 
                 9.2E−10 
                 2.8E+05 
                 3.4E−04 
                 1.2E−09 
               
               
                   
                   
                 2 
                 2.8E+05 
                 2.8E−04 
                 9.9E−10 
                 2.9E+05 
                 3.6E−04 
                 1.3E−09 
               
               
                   
                 602 
                 1 
                 2.9E+05 
                 1.7E−04 
                 5.8E−10 
                 3.0E+05 
                 1.7E−04 
                 5.8E−10 
               
               
                   
                   
                 2 
                 2.9E+05 
                 1.6E−04 
                 5.3E−10 
                 3.1E+05 
                 1.7E−04 
                 5.5E−10 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
               
                   
               
               
                 Kinetic affinity parameters of Trastuzumab variants similar to 
               
               
                 table 3. Here the samples are incubated at 40° in 40 mM 
               
               
                 Histidin, 150 mM NaCl, pH 6.0 for the same time intervals as before. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 t0 
                 t1 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 pH 6 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 wt 
                 1 
                 2.8E+05 
                 9.5E−05 
                 3.4E−10 
                 3.0E+05 
                 1.5E−04 
                 5.1E−10 
               
               
                   
                 2 
                 2.8E+05 
                 8.6E−05 
                 3.1E−10 
                 2.8E+05 
                 2.1E−04 
                 7.7E−10 
               
               
                 602 
                 1 
                 2.9E+05 
                 1.4E−04 
                 4.8E−10 
                 3.0E+05 
                 1.6E−04 
                 5.2E−10 
               
               
                   
                 2 
                 2.8E+05 
                 1.4E−04 
                 5.0E−10 
                 2.9E+05 
                 1.5E−04 
                 5.1E−10 
               
               
                   
               
            
           
           
               
               
               
            
               
                   
                 t2 
                 t3 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 pH 6 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 wt 
                 1 
                 2.5E+05 
                 3.1E−04 
                 1.2E−09 
                 2.3E+05 
                 4.0E−04 
                 1.7E−09 
               
               
                   
                   
                 2 
                 2.6E+05 
                 3.3E−04 
                 1.3E−09 
                 2.4E+05 
                 4.1E−04 
                 1.8E−09 
               
               
                   
                 602 
                 1 
                 3.0E+05 
                 1.6E−04 
                 5.3E−10 
                 3.0E+05 
                 1.8E−04 
                 5.9E−10 
               
               
                   
                   
                 2 
                 2.9E+05 
                 1.6E−04 
                 5.5E−10 
                 2.9E+05 
                 1.7E−04 
                 5.7E−10 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
               
                   
               
               
                 Kinetic affinity parameters of Trastuzumab variants similar to table 3. Here 
               
               
                 the samples are incubated at 40° in 40 mM Histidin, 150 mM NaCl, pH 5.0 
               
               
                 for the same time intervals as before. Samples included are the resynthesized 
               
               
                 Trastuzumab (wt), the D98E T31V variant in the heavy and light chain, respectively 
               
               
                 (named clone 602. This was produced in CHO instead of HEK). Also the light 
               
               
                 chain variants N30S, and N30T (both have the D98E heavy chain variant) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 t0 
                 t1 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 pH 5 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 602 
                 1 
                 2.20E+05 
                 1.40E−04 
                 6.34E−10 
                 2.32E+05 
                 9.14E−05 
                 3.95E−10 
               
               
                 CHO 
                 2 
                 2.32E+05 
                 2.13E−04 
                 9.21E−10 
                 2.10E+05 
                 1.54E−04 
                 7.35E−10 
               
               
                 N30T 
                 1 
                 2.19E+05 
                 2.11E−04 
                 9.64E−10 
                 2.21E+05 
                 1.46E−04 
                 6.61E−10 
               
               
                   
                 2 
                 2.18E+05 
                 2.61E−04 
                 1.20E−09 
                 2.05E+05 
                 1.85E−04 
                 9.02E−10 
               
               
                 N30S * 
                 1 
                 2.59E+05 
                 2.59E−19 
                 1.00E−24 
                 2.54E+05 
                 1.93E−05 
                 7.58E−11 
               
               
                   
                 2 
                 2.44E+05 
                 2.36E−17 
                 9.68E−23 
                 2.42E+05 
                 3.47E−05 
                 1.43E−10 
               
               
                 wt 
                 1 
                 2.51E+05 
                 1.60E−04 
                 6.39E−10 
                 2.30E+05 
                 3.62E−04 
                 1.58E−09 
               
               
                   
                 2 
                 2.29E+05 
                 1.93E−04 
                 8.43E−10 
                 2.13E+05 
                 2.66E−04 
                 1.25E−09 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 t2 
                   
                 t3 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 pH 5 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 602 
                 1 
                 2.32E+05 
                 2.36E−04 
                 1.02E−09 
                 2.01E+05 
                 9.67E−05 
                 4.81E−10 
               
               
                 CHO 
                 2 
                 2.37E+05 
                 2.35E−04 
                 9.89E−10 
                 2.08E+05 
                 1.30E−04 
                 6.25E−10 
               
               
                 N30T 
                 1 
                 2.20E+05 
                 1.77E−04 
                 8.05E−10 
                 2.31E+05 
                 2.87E−04 
                 1.24E−09 
               
               
                   
                 2 
                 2.01E+05 
                 2.02E−04 
                 1.00E−09 
                 2.27E+05 
                 2.82E−04 
                 1.24E−09 
               
               
                 N30S * 
                 1 
                 2.49E+05 
                 1.16E−16 
                 4.64E−22 
                 2.54E+05 
                 4.11E−18 
                 1.62E−23 
               
               
                   
                 2 
                 2.37E+05 
                 3.71E−16 
                 1.57E−21 
                 2.37E+05 
                 6.06E−17 
                 2.55E−22 
               
               
                 wt 
                 1 
                 2.38E+05 
                 6.47E−04 
                 2.72E−09 
                 2.33E+05 
                 7.72E−04 
                 3.32E−09 
               
               
                   
                 2 
                 2.26E+05 
                 6.88E−04 
                 3.05E−09 
                 2.18E+05 
                 7.91E−04 
                 3.62E−09 
               
               
                   
               
               
                 * Due to slow dissociation rates, the off-rates and the dissociation constant contain a high degree of uncertainty. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
               
                   
               
               
                 Kinetic affinity parameters of Trastuzumab variants similar to table 3. Here 
               
               
                 the samples are incubated at 40° in 40 mM Histidin, 150 mM NaCl, pH 6.0 
               
               
                 for the same time intervals as before. Samples included are the resynthesized 
               
               
                 Trastuzumab (wt), the D98E T31V variant in the heavy and light chain, respectively 
               
               
                 (named clone 602. This was produced in CHO instead of HEK). Also the light 
               
               
                 chain variants N30S, and N30T (both have the D98E heavy chain variant) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 t0 
                 t1 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 pH 6 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 602 
                 1 
                 2.07E+05 
                 9.33E−05 
                 4.51E−10 
                 2.30E+05 
                 1.37E−04 
                 5.94E−10 
               
               
                 CHO 
                 2 
                 2.16E+05 
                 1.97E−04 
                 9.12E−10 
                 2.16E+05 
                 2.02E−04 
                 9.32E−10 
               
               
                 N30T 
                 1 
                 2.24E+05 
                 2.45E−04 
                 1.09E−09 
                 2.03E+05 
                 1.24E−04 
                 6.10E−10 
               
               
                   
                 2 
                 2.31E+05 
                 2.71E−04 
                 1.17E−09 
                 1.93E+05 
                 1.57E−04 
                 8.13E−10 
               
               
                 N30S * 
                 1 
                 2.56E+05 
                 1.18E−17 
                 4.62E−23 
                 2.65E+05 
                 2.77E−05 
                 1.04E−10 
               
               
                   
                 2 
                 2.48E+05 
                 8.14E−06 
                 3.28E−11 
                 2.51E+05 
                 4.23E−05 
                 1.68E−10 
               
               
                 wt 
                 1 
                 2.23E+05 
                 7.43E−05 
                 3.33E−10 
                 2.22E+05 
                 4.60E−04 
                 2.07E−09 
               
               
                   
                 2 
                 2.23E+05 
                 7.98E−05 
                 3.59E−10 
                 2.21E+05 
                 4.03E−04 
                 1.82E−09 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 t2 
                   
                 t3 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 pH 6 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 602 
                 1 
                 2.19E+05 
                 1.29E−04 
                 5.87E−10 
                 2.14E+05 
                 1.50E−04 
                 7.01E−10 
               
               
                 CHO 
                 2 
                 2.07E+05 
                 1.52E−04 
                 7.31E−10 
                 2.13E+05 
                 1.60E−04 
                 7.50E−10 
               
               
                 N30T 
                 1 
                 2.11E+05 
                 2.27E−04 
                 1.08E−09 
                 2.08E+05 
                 2.01E−04 
                 9.67E−10 
               
               
                   
                 2 
                 2.05E+05 
                 2.55E−04 
                 1.24E−09 
                 2.03E+05 
                 1.98E−04 
                 9.75E−10 
               
               
                 N30S * 
                 1 
                 2.38E+05 
                 8.97E−19 
                 3.77E−24 
                 2.36E+05 
                 1.84E−05 
                 7.80E−11 
               
               
                   
                 2 
                 2.25E+05 
                 4.30E−17 
                 1.91E−22 
                 2.33E+05 
                 3.41E−05 
                 1.46E−10 
               
               
                 wt 
                 1 
                 2.31E+05 
                 9.24E−04 
                 4.00E−09 
                 1.89E+05 
                 9.91E−04 
                 5.24E−09 
               
               
                   
                 2 
                 2.20E+05 
                 9.31E−04 
                 4.24E−09 
                 1.78E+05 
                 9.95E−04 
                 5.58E−09 
               
               
                   
               
               
                 * Due to slow dissociation rates, the off-rates and the dissociation constant contain a high degree of uncertainty. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
               
                   
               
               
                 Kinetic affinity parameters of Trastuzumab variants similar to table 3. Here 
               
               
                 the samples are incubated at 40° in 40 mM Histidin, 150 mM NaCl, pH 7.4 
               
               
                 for the same time intervals as before. Samples included are the resynthesized 
               
               
                 Trastuzumab (wt), the D98E T31V variant in the heavy and light chain, respectively 
               
               
                 (named clone 602. This was produced in CHO instead of HEK). Also the light 
               
               
                 chain variants N30S, and N30T (both have the D98E heavy chain variant) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 t0 
                 t1 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 pH 7.4 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 602 
                 1 
                 2.15E+05 
                 1.23E−04 
                 5.71E−10 
                 2.32E+05 
                 1.80E−04 
                 7.78E−10 
               
               
                 CHO 
                 2 
                 2.17E+05 
                 2.08E−04 
                 9.55E−10 
                 2.19E+05 
                 2.31E−04 
                 1.06E−09 
               
               
                 N30T 
                 1 
                 2.46E+05 
                 2.19E−04 
                 8.87E−10 
                 2.15E+05 
                 1.86E−04 
                 8.65E−10 
               
               
                   
                 2 
                 2.20E+05 
                 2.30E−04 
                 1.04E−09 
                 2.00E+05 
                 2.03E−04 
                 1.02E−09 
               
               
                 N30S 
                 1 
                 2.58E+05 
                 1.35E−06 
                 5.25E−12 
                 2.60E+05 
                 2.55E−05 
                 9.82E−11 
               
               
                   
                 2 
                 2.36E+05 
                 2.24E−05 
                 9.46E−11 
                 2.49E+05 
                 6.31E−18 
                 2.53E−23 
               
               
                 wt 
                 1 
                 2.25E+05 
                 7.49E−05 
                 3.33E−10 
                 1.99E+05 
                 8.80E−04 
                 4.43E−09 
               
               
                   
                 2 
                 2.12E+05 
                 9.85E−05 
                 4.65E−10 
                 2.00E+05 
                 8.78E−04 
                 4.40E−09 
               
               
                   
               
            
           
           
               
               
               
            
               
                   
                 t2 
                 t3 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 pH 7.4 
                 ka 
                 kd 
                 KD 
                 ka 
                 kd 
                 KD 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 602 
                 1 
                 1.97E+05 
                 1.05E−04 
                 5.34E−10 
                 2.02E+05 
                 2.24E−04 
                 1.11E−09 
               
               
                   
                 CHO 
                 2 
                 1.82E+05 
                 1.33E−04 
                 7.30E−10 
                 2.00E+05 
                 2.14E−04 
                 1.07E−09 
               
               
                   
                 N30T 
                 1 
                 2.12E+05 
                 2.64E−04 
                 1.25E−09 
                 1.94E+05 
                 1.41E−04 
                 7.26E−10 
               
               
                   
                   
                 2 
                 1.98E+05 
                 2.84E−04 
                 1.44E−09 
                 1.73E+05 
                 1.54E−04 
                 8.87E−10 
               
               
                   
                 N30S 
                 1 
                 2.43E+05 
                 7.28E−21 
                 2.99E−26 
                 2.37E+05 
                 6.30E−05 
                 2.66E−10 
               
               
                   
                   
                 2 
                 2.27E+05 
                 1.24E−05 
                 5.46E−11 
                 2.21E+05 
                 6.55E−05 
                 2.97E−10 
               
               
                   
                 wt 
                 1 
                 1.64E+05 
                 1.35E−03 
                 8.27E−09 
                 1.55E+05 
                 1.79E−03 
                 1.15E−08 
               
               
                   
                   
                 2 
                 1.67E+05 
                 1.29E−03 
                 7.72E−09 
                 1.45E+05 
                 1.63E−03 
                 1.12E−08 
               
               
                   
                   
               
               
                   
                 * Due to slow dissociation rates, the off-rates and the dissociation constant contain a high degree of uncertainty. 
               
            
           
         
       
     
     Example 5: Binding of Trastuzumab and Trastuzumab Stabilization Variants after Stress to KPL-4 Cells 
     Binding 
     KPL-4 cells were harvested and resuspended in FACS buffer. 0.2 Mio cells were seeded into a 96 well round bottom plate. The plate was centrifuged at 400 g for 3 min to pellet the cells. The supernatant was removed and the cells were resuspended in 40 μl of the diluted antibodies. The plate was incubated for 30 min at 4° C. to allow binding of the antibodies. To remove unbound antibodies the cells were centrifuged again and washed twice with FACS buffer. To detect the antibodies the cells were resuspended in 12 μl diluted secondary goat anti-human Fc specific FITC-labeled secondary antibody (Jackson ImmunoResearch #109-096-098) and incubated again for 30 min at 4° C. Afterwards the cells were washed twice with FACS buffer, resuspended in 200 μl FACS buffer and the fluorescence was measured with BD CantoII. 
     ADCC 
     Target cells were harvested, washed, stained with calcein (Invitrogen), resuspended in AIM V® medium (Life Technologies), and plated at a concentration of 3×10 4  cells/well. The respective antibody dilutions were added in triplicates to the cells and incubated for 10 min before addition of the effector cells (peripheral blood mononuclear effector cells [PBMCs]). Effector (E) and target (T) cells were then incubated for the indicated time at 37° C. at the indicated E:T ratio (triplicates for all samples). After incubation the cells were washed once with PBS and then lysed with borate buffer. Calcein retention was measured in a Wallac Victor3 1420 Multilabel Counter. ADCC was calculated using the following formula: 
     
       
         
           
             
               Percentage 
                
               
                   
               
                
               ADCC 
             
             = 
             
               
                 ( 
                 
                   [ 
                   
                     
                       
                         sample 
                          
                         
                             
                         
                          
                         release 
                       
                       - 
                       
                         spontaneous 
                          
                         
                             
                         
                          
                         release 
                       
                     
                     
                       
                         maximal 
                          
                         
                             
                         
                          
                         release 
                       
                       - 
                       
                         spontaneous 
                          
                         
                             
                         
                          
                         release 
                       
                     
                   
                   ] 
                 
                 ) 
               
               × 
               100. 
             
           
         
       
     
     Spontaneous release, corresponding to target cells incubated with effector cells without antibody, was defined as 0% cytotoxicity, and maximal release (target cells lysed with 1% Triton X-100) was defined as 100% cytotoxicity. The average percentage of ADCC and standard deviations of the triplicates of each experiment were calculated. 
     Results are shown in  FIGS. 7A and 7B to 9 . 
     Example 6: Generation of Herceptarg CrossMab and Framework Grafting on Novel LC06 Based Framework to Achieve Less Mispairing 
     Gene Synthesis 
     Desired gene segments were prepared by Geneart AG (Regensburg, Germany) from synthetic oligonucleotides and PCR products by automated gene synthesis. The gene segments encoding heavy or light chains with C-terminal attachment of scFv antibody fragments, “knobs-into-hole” antibody heavy chains carrying S354C and T366W mutations and “knobs-into-hole” heavy chains carrying Y349C, T366S, L368A and Y407V mutations in the CH3 domain in combination with unmodified VH domains, crossed C kappa domains or scFab antibody fragments as well as unmodified antibody light chains or CH1 domain exchanged light chains are flanked by singular restriction endonuclease cleavage sites (BamHI-XbaI, BamHI-XmnI or BamHI-KpnI) and were cloned into pGA18 (ampR) plasmids. The plasmid DNA was purified from transformed bacteria and concentration determined by UV spectroscopy. The DNA sequence of the subcloned gene fragments was confirmed by DNA sequencing. All constructs were designed with a 5′-end DNA sequence coding for a leader peptide (MGWSCIILFLVATATGVHS), which targets proteins for secretion in eukaryotic cells. 
     Construction of the Expression Plasmids 
     The expression vector that was used for the construction of all “knobs-into-hole” heavy chain as well as antibody light chain encoding expression plasmids comprises the following elements:
         a hygromycin resistance gene as a selection marker,   an origin of replication, oriP, of Epstein-Barr virus (EBV),   an origin of replication from the vector pUC18 which allows replication of this plasmid in  E. coli      a beta-lactamase gene which confers ampicillin resistance in  E. coli,      the immediate early enhancer and promoter from the human cytomegalovirus (HCMV),   the human 1-immunoglobulin polyadenylation (“poly A”) signal sequence, and unique BamHI and XbaI restriction sites.       

     The immunoglobulin genes comprising heavy or light chains with C-terminal attachment of scFv antibody fragments, “knobs-into-hole” heavy chains with unmodified VH domains, crossed C kappa domains or scFab fragments as well as unmodified light chains or CH1 domain exchanged light chains were prepared by gene synthesis and cloned into pGA18 (ampR) plasmids as described. The pG18 (ampR) plasmids carrying the synthesized DNA segments and the expression vector were digested with BamHI and XbaI, BamHI and XmnI or BamHI and KpnI restriction enzymes (Roche Molecular Biochemicals) and subjected to agarose gel electrophoresis. Purified heavy or light chains with C-terminal attachment of scFv antibody fragments, “knobs-into-hole” heavy and unmodified or domain exchanged light chain encoding DNA segments were then ligated to the isolated expression vector BamHI/XbaI, BamHI/XmnI or BamHI/KpnI fragment resulting in the final expression vectors. The final expression vectors were transformed into  E. coli  cells, expression plasmid DNA was isolated (Miniprep) and subjected to restriction enzyme analysis and DNA sequencing. Correct clones were grown in 150 ml LB-Amp medium, again plasmid DNA was isolated (Maxiprep) and sequence integrity confirmed by DNA sequencing. 
     Transient Expression of Bispecific Antibodies in HEK293 Cells 
     Recombinant bispecific antibodies were expressed by transient transfection of human embryonic kidney 293-F cells using the FreeStyle™ 293 Expression System according to the manufacturer&#39;s instruction (Invitrogen, USA). Briefly, suspension FreeStyle™ 293-F cells were cultivated in FreeStyle™ 293 Expression medium at 37° C./8% CO 2  and the cells were seeded in fresh medium at a density of 1×10 6  viable cells/ml one day before transfection. For transfection, DNA was prepared in 10 ml Dulbecco&#39;s PBS (PAA, Austria) using 162.5 μl of 293-Free™ Transfection Reagent (Merck, USA) and 125 μg of heavy with N-terminal attachment of scFab encoding DNA in a plasmid ratio of 1:1 with “Knobs-into-hole” heavy chain 1 and 2 and light chain plasmid DNA in a 1:1:1 molar ratio in 250 ml final transfection volume. For transfection of Cross Mabs, a plasmid ratio of 1:1:1:1, 1:1:1:2, 1:1:1:4, 1:1:1:8 of “Knobs-into-hole” heavy chain 1:unmodified light chain:C kappa domain exchanged “Knobs-into-hole” heavy chain 2:CH1 domain exchanged light chain was prepared. The CH1-VL light chain plasmid ration was used at 1×, 2×, 4× and 8× molar ratios to assess the optimization of chain pairing using a combination of CE-SDS and Q-TOF spectrometry. Antibody containing cell culture supernatants were harvested 7 days after transfection by centrifugation at 14000 g for 30 minutes and filtered through a sterile filter (0.22 μm). Supernatants were stored at −20° C. until purification. The sequences of the resulting antibodies are shown below in table 8. 
     Preparation of the Glycoengineered Derivatives of Bispecific &lt;Her2GlyMab&gt; Antibodies 
     Glycoengineered derivatives of bispecific &lt;Her2GlyMab&gt; antibodies were produced by co-transfecting HEK293-EBNA cells with the mammalian antibody heavy and light chain expression vectors using a calcium phosphate-transfection approach. Exponentially growing HEK293-EBNA cells were transfected by the calcium phosphate method. For the production of the glycoengineered antibody, the cells were co-transfected with a plasmid for a fusion GnTIII polypeptide expression and a second plasmid for mannosidase II expression, respectively. Plasmid ratios of bispecific antibodies were added as described in the material and methods section above. Cells were grown as adherent monolayer cultures in T flasks using DMEM culture medium supplemented with 10% FCS, and were transfected when they were between 50 and 80% confluent. For the transfection of a T75 flask, 7.5 (to 8) million cells were seeded 24 hours before transfection in ca 14 ml DMEM culture medium supplemented with FCS (at 10% V/V final), (eventually 250 μg/ml neomycin) and cells were placed at 37° C. in an incubator with a 5% CO2 atmosphere overnight. For each T75 flask to be transfected, a solution of DNA, CaCl2 and water was prepared by mixing 47 μg total plasmid vector DNA, 235 μl of a 1M CaCl2 solution, and adding water to a final volume of 469 μl. To this solution, 469 μl of a 50 mM HEPES, 280 mM NaCl, 1.5 mM Na2HPO4 solution at pH 7.05 were added, mixed immediately for 10 sec and left to stand at room temperature for 20 sec. The suspension was diluted with ca. 12 ml of DMEM supplemented with 2% FCS, and added to the T75 in place of the existing medium. The cells were incubated at 37° C., 5% CO2 for about 17 to 20 hours, then medium was replaced with ca. 12 ml DMEM, 10% FCS. The conditioned culture medium was harvested 5 to 7 days post-transfection centrifuged for 5 min at 210-300*g, sterile filtered through a 0.22 μm filter (or alternatively centrifuged for 5 min at 1200 rpm, followed by a second centrifugation for 10 min at 4000 rpm) and kept at 4° C. 
     Glycoengineered antibodies were purified and formulated as described above for the non-glycoengineered antibodies. The oligosaccharides attached to the Fc region of the antibodies were analysed as described below to determine the amount of fucose. 
     Framework Grafting 
     Rationale: Similar framework of trastuzumab and pertuzumab allows mis-pairing of light chains: Both Trastuzumab and Pertuzumab have a V H III V L kI framework and therefore the light chain interface affinity to both heavy chains is identical. 
     In order to avoid mispairing of light chains in the crossMab Herceptarg bispecific antibody, the Trastuzumab framework of “CrossMabXPer Her2GlyMab” was exchanged with the framework of the non-related antibody LC06. Trastuzumab is related to germlines hVH3_66 and hVK1D_39 whereas the antibody LC06 corresponds to germlines hVbase_VH1_1 germline and hVL_3, respectively. Pertuzumab is related to hVH3_23 and hVK1D_13 showing a very similar framework system compared to Trastuzumab. 
     Both germline acceptor frameworks of LC06 are different from the Trastuzumab framework, especially, the LC06 lambda light chain, compared to the Trastzumab kappa light chain. The antibody Fab crystal structure of Trastuzumab has been superimposed and the compatibility of the framewords have been structurally evaluated. CDRI, II and III of the Trastuzumab light chain were grafted onto the new Lambda framework of LC06. Mutations included were D98E and N30S, the N30S was used in favour of T31V because of the increase in affinity to the Her2 extracellular domain with the N30S modification. It was thought that any reduction of the Kd caused by the CDR grafting could be compensated by the use of the N30S mutation. Some accommodations in the acceptor frameworks were required in order to get the CDRs in their biological active conformation; for example A (LC06 lambda) at the Kabat position 71 of the light chain has been backmutated to F (Trastuzumab kappa). The original VHIII-VLkI (Kappa I family) framework of Pertuzumab was maintained. The resulting bispecific antibody is depicted as “CrossMab-CDRG Her2GlyMab”, with sequences as shown in Table 8 below. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Sequences of Herceptarg CrossMab bispecific antibodies. 
               
            
           
           
               
               
               
            
               
                 Construct 
                 SEQ ID NO 
                 Sequence 
               
               
                   
               
            
           
           
               
            
               
                 OAscFab1 Her2GlyMab 
               
            
           
           
               
               
               
            
               
                 scFab 
                 133 
                 diqmtqspsslsasvgdrvtitcrasqdvnvavawyqqkpgkapklliysasflysgvpsrfsg 
               
               
                 Trastuzumab 
                   
                 srsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikrtvaapsvfifppsdeqlksgtas 
               
               
                 heavy chain 1 
                   
                 vvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyac 
               
               
                   
                   
                 evthqglsspvtksfnrgecggggsggggsggggsggggsggggsggggsggevqlvesgg 
               
               
                   
                   
                 glvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryadsvkgrftisadt 
               
               
                   
                   
                 skntaylqmnslraedtavyycsrwggegfyamdywgqgtlvtvssastkgpsvfplapssk 
               
               
                   
                   
                 stsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvpssslgtqtyi 
               
               
                   
                   
                 cnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlmisrtpevtcv 
               
               
                   
                   
                 vvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlngkeykck 
               
               
                   
                   
                 vsnkalpapiektiskakgqprepqvytlppcrdeltknqvslwclvkgfypsdiavewesng 
               
               
                   
                   
                 qpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspgk 
               
               
                   
               
               
                 Pertuzumab 
                 134 
                 evqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkglewvadvnpnsggsiyn 
               
               
                 heavy chain 2 
                   
                 qrfkgrftlsvdrskntlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtvssastkgps 
               
               
                   
                   
                 vfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvp 
               
               
                   
                   
                 ssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlm 
               
               
                   
                   
                 isrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsyltvlhqdwl 
               
               
                   
                   
                 ngkeykckvsnkalpapiektiskakgqprepqvctlppsrdeltknqvslscavkgfypsdi 
               
               
                   
                   
                 avewesngqpennykttppvldsdgsfflvskltvdksrwqqgnvfscsvmhealhnhytq 
               
               
                   
                   
                 kslslspgk 
               
               
                   
               
               
                 Pertuzumab 
                 135 
                 diqmtqspsslsasvgdrytitckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsg 
               
               
                 light chain 1 
                   
                 sgsgtdftltisslqpedfatyycqqyyiypytfgqgtkveikrtvaapsvfifppsdeqlksgtas 
               
               
                   
                   
                 vvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyac 
               
               
                   
                   
                 evthqglsspytksfnrgec 
               
               
                   
               
            
           
           
               
            
               
                 OAscFab2 Her2GlyMab 
               
            
           
           
               
               
               
            
               
                 Pertuzumab 
                 136 
                 evqlvesggglyqpggslrlscaasgftftdytmdwvrqapgkglewvadynpnsggsiyn 
               
               
                 heavy chain 1 
                   
                 qrfkgrftlsydrskntlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtvssastkgps 
               
               
                   
                   
                 vfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvp 
               
               
                   
                   
                 ssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlm 
               
               
                   
                   
                 isrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwl 
               
               
                   
                   
                 ngkeykckvsnkalpapiektiskakgqprepqvctlppsrdeltknqvslscavkgfypsdi 
               
               
                   
                   
                 avewesngqpennykttppvldsdgsfflvskltvdksrwqqgnvfscsvmhealhnhytq 
               
               
                   
                   
                 kslslspgk 
               
               
                   
               
               
                 Pertuzumab 
                 137 
                 diqmtqspsslsasvgdrvtitckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsg 
               
               
                 light chain 1 
                   
                 sgsgtdftltisslqpedfatyycqqyyiypytfgqgtkveikrtvaapsvfifppsdeqlksgtas 
               
               
                   
                   
                 vvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyac 
               
               
                   
                   
                 evthqglsspvtksfnrgec 
               
               
                   
               
               
                 scFab 
                 138 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryads 
               
               
                 Trastuzumab 
                   
                 vkgrftisadtskntaylqmnslraedtavyycsrwggegfyamdywgqgtlvtvssastkg 
               
               
                 heavy chain 2 
                   
                 psvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvt 
               
               
                   
                   
                 vpssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdt 
               
               
                   
                   
                 lmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhq 
               
               
                   
                   
                 dwlngkeykckvsnkalpapiektiskakgqprepqvytlppcrdeltknqvslwclvkgfy 
               
               
                   
                   
                 psdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnyfscsvmhealhn 
               
               
                   
                   
                 hytqkslslspgkggggsggggsggggsggggsggggsggggsggdiqmtqspsslsasvg 
               
               
                   
                   
                 drvtitcrasqdvnvavawyqqkpgkapklliysasflysgvpsrfsgsrsgtdftltisslqped 
               
               
                   
                   
                 fatyycqqhyttpptfgqgtkveikrtvaapsvfifppsdeqlksgtasvvcllnnfypreakvq 
               
               
                   
                   
                 wkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyacevthqglsspvtksfnr 
               
               
                   
                   
                 gec 
               
               
                   
               
            
           
           
               
            
               
                 OAscFabPer1 Her2GlyMab 
               
            
           
           
               
               
               
            
               
                 scFab 
                 139 
                 evqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkglewvadynpnsggsiyn 
               
               
                 Pertuzumab 
                   
                 qrfkgrftlsvdrskntlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtvssastkgps 
               
               
                 heavy chain 1 
                   
                 vfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtyp 
               
               
                   
                   
                 ssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlm 
               
               
                   
                   
                 isrtpevtcvvvdvshedpevkfnwyydgvevhnaktkpreeqynstyrvvsvltvlhqdwl 
               
               
                   
                   
                 ngkeykckvsnkalpapiektiskakgqprepqvctlppsrdeltknqvslscavkgfypsdi 
               
               
                   
                   
                 avewesngqpennykttppvldsdgsfflvskltvdksrwqqgnvfscsvmhealhnhytq 
               
               
                   
                   
                 kslslspgkggggsggggsggggsggggsggggsggggsggdiqmtqspsslsasvgdrvt 
               
               
                   
                   
                 itckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsgsgsgtdftltisslqpedfaty 
               
               
                   
                   
                 ycqqyyiypytfgqgtkveikrtvaapsvfifppsdeqlksgtasvvcllnnfypreakvqwk 
               
               
                   
                   
                 vdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyaceythqglsspvtksfnrgec 
               
               
                   
               
               
                 Trastuzumab 
                 140 
                 evqlvesggglyqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryads 
               
               
                 heavy chain 2 
                   
                 vkgrftisadtskntaylqmnslraedtavyycsrwggegfyamdywgqgtlvtvssastkg 
               
               
                   
                   
                 psvfplapsskstsggtaalgclykdyfpepytvswnsgaltsgvhtfpavlqssglyslssvvt 
               
               
                   
                   
                 vpssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdt 
               
               
                   
                   
                 lmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhq 
               
               
                   
                   
                 dwlngkeykckvsnkalpapiektiskakgqprepqvytlpperdeltknqvslwclvkgfy 
               
               
                   
                   
                 psdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhn 
               
               
                   
                   
                 hytqkslslspgk 
               
               
                   
               
               
                 Trastuzumab 
                 141 
                 diqmtqspsslsasvgdrvtitcrasqdvnvavawyqqkpgkapklliysasflysgvpsrfsg 
               
               
                 light chain 2 
                   
                 srsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikrtvaapsyfifppsdeqlksgtas 
               
               
                   
                   
                 vvcllnnfypreakvqwkydnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyac 
               
               
                   
                   
                 evthqglsspvtksfnrge 
               
               
                   
               
            
           
           
               
            
               
                 OAscFabPer2 Her2GlyMab 
               
            
           
           
               
               
               
            
               
                 Trastuzumab 
                 142 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryads 
               
               
                 heavy chain 1 
                   
                 vkgrftisadtskntaylqmnslraedtavyycsrwggegfyamdywgqgtlvtvssastkg 
               
               
                   
                   
                 psvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvt 
               
               
                   
                   
                 vpssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdt 
               
               
                   
                   
                 lmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhq 
               
               
                   
                   
                 dwlngkeykckvsnkalpapiektiskakgqprepqvytlpperdeltknqvslwclvkgfy 
               
               
                   
                   
                 psdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhn 
               
               
                   
                   
                 hytqkslslspgk 
               
               
                   
               
               
                 Trastuzumab 
                 143 
                 diqmtqspsslsasvgdrvtitcrasqdvnyavawyqqkpgkapklliysasflysgvpsrfsg 
               
               
                 light chain 1 
                   
                 srsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikrtvaapsvfifppsdeqlksgtas 
               
               
                   
                   
                 vvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyac 
               
               
                   
                   
                 eythqglsspvtksfnrgec 
               
               
                   
               
               
                 scFab 
                 144 
                 evqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkglewvadvnpnsggsiyn 
               
               
                 Pertuzumab 
                   
                 qrfkgrftlsvdrskntlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtvssastkgps 
               
               
                 heavy chain 2 
                   
                 vfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvp 
               
               
                   
                   
                 ssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdtlm 
               
               
                   
                   
                 isrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwl 
               
               
                   
                   
                 ngkeykckvsnkalpapiektiskakgqprepqvctlppsrdeltknqvslscavkgfypsdi 
               
               
                   
                   
                 avewesngqpennykttppvldsdgsfflvskltvdksrwqqgnvfscsvmhealhnhytq 
               
               
                   
                   
                 kslslspgkggggsggggsggggsggggsggggsggggsggdiqmtqspsslsasvgdrvt 
               
               
                   
                   
                 itckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsgsgsgtdftltisslqpedfaty 
               
               
                   
                   
                 ycqqyyiypytfgqgtkveikrtvaapsvfifppsdeqlksgtasvvcllnnfypreakvqwk 
               
               
                   
                   
                 vdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyacevthqglsspvtksfnrgec 
               
               
                   
               
            
           
           
               
            
               
                 CrossMab-XPer Her2GlyMab 
               
            
           
           
               
               
               
            
               
                 XPertuzumab 
                 109 
                 evqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkglewvadvnpnsggsiyn 
               
               
                 heavy chain 
                   
                 qrfkgrftlsvdrskntlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtvssasvaaps 
               
               
                   
                   
                 vfifppsdeqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsst 
               
               
                   
                   
                 ltlskadyekhkvyacevthqglsspvtksfnrgecdkthtcppcpapellggpsvflfppkpk 
               
               
                   
                   
                 dtlmisrtpevtcvvvdvshedpevkfnwyydgvevhnaktkpreeqynstyrvvsvltvlh 
               
               
                   
                   
                 qdwlngkeykckvsnkalpapiektiskakgqprepqvytlppcrdeltknqvslwclvkgf 
               
               
                   
                   
                 ypsdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealh 
               
               
                   
                   
                 nhytqkslslspgk 
               
               
                   
               
               
                 XPertuzumab 
                 110 
                 diqmtqspsslsasvgdrvtitckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsg 
               
               
                 light chain 
                   
                 sgsgtdftltisslqpedfatyycqqyyiypytfgqgtkveikssastkgpsvfplapsskstsggt 
               
               
                   
                   
                 aalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtypssslgtqtyicnvnh 
               
               
                   
                   
                 kpsntkvdkkvepksc 
               
               
                   
               
               
                 Trastuzumab 
                 96 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewyariyptngytryads 
               
               
                 heavy chain 
                   
                 vkgrftisadtskntaylqmnslraedtavyycsrwggegfyamdywgqgtlvtvssastkg 
               
               
                 (VH D98E  CH1) 
                   
                 psvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvt 
               
               
                   
                   
                 vpssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdt 
               
               
                   
                   
                 lmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhq 
               
               
                   
                   
                 dwlngkeykckvsnkalpapiektiskakgqprepqvctlppsrdeltknqvslscavkgfyp 
               
               
                   
                   
                 sdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnh 
               
               
                   
                   
                 ytqkslslspgk 
               
               
                   
               
               
                 Trastuzumab 
                 86 
                 diqmtqspsslsasvgdrvtitcrasqdvnvavawyqqkpgkapklliysasflysgvpsrfsg 
               
               
                 light chain 
                   
                 srsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikrtvaapsvfifppsdeqlksgtas 
               
               
                 (VL T31V  CL) 
                   
                 vvcllnnfypreakvqwkydnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyac 
               
               
                   
                   
                 evthqglsspvtksfnrgec 
               
               
                   
               
            
           
           
               
            
               
                 CrossMab-XTra Her2GlyMab 
               
            
           
           
               
               
               
            
               
                 Pertuzumab 
                 119 
                 evqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkglewvadvnpnsggsiyn 
               
               
                 heavy chain 
                   
                 qrfkgrftlsvdrskntlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtyssasvaaps 
               
               
                   
                   
                 vfifppsdeqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsst 
               
               
                   
                   
                 ltlskadyekhkvyacevthqglsspvtksfurgecdkthtcppcpapellggpsvflfppkpk 
               
               
                   
                   
                 dtlmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlh 
               
               
                   
                   
                 qdwlngkeykckvsnkalpapiektiskakgqprepqvctlppsrdeltknqvslscavkgfy 
               
               
                   
                   
                 psdiavewesngqpennykttppvldsdgsfflvskltvdksrwqqgnvfscsvmhealhn 
               
               
                   
                   
                 hytqkslslspgk 
               
               
                   
               
               
                 Pertuzumab 
                 120 
                 diqmtqspsslsasvgdrvtitckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsg 
               
               
                 light chain 
                   
                 sgsgtdftltisslqpedfatyycqqyyiypytfgqgtkveikrtvaapsvfifppsdeqlksgtas 
               
               
                   
                   
                 vvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyac 
               
               
                   
                   
                 evthqglsspvtksfnrgec 
               
               
                   
               
               
                 XTrastuzuma 
                 121 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryads 
               
               
                 b heavy chain 
                   
                 vkgrftisadtskntaylqmnslraedtavyycsrwggegfyamdywgqgtlvtvssastkg 
               
               
                   
                   
                 psvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvt 
               
               
                   
                   
                 vpssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpkdt 
               
               
                   
                   
                 lmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhq 
               
               
                   
                   
                 dwlngkeykckvsnkalpapiektiskakgqprepqvytlppcrdeltknqvslwclvkgfy 
               
               
                   
                   
                 psdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhn 
               
               
                   
                   
                 hytqkslslspgk 
               
               
                   
               
               
                 XTrastuzuma 
                 122 
                 diqmtqspsslsasvgdrvtitcrasqdvnvavawyqqkpgkapklliysasflysgvpsrfsg 
               
               
                 b light chain 
                   
                 srsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikssastkgpsvfplapsskstsggta 
               
               
                   
                   
                 algclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvpssslgtqtyicnvnhk 
               
               
                   
                   
                 psntkvdkkvepksc 
               
               
                   
               
            
           
           
               
            
               
                 Optimized Trastuzumab sequences: CrossMab-XTra Her2GlyMab and CrossMab-XPer Her2GlyMab 
               
            
           
           
               
               
               
            
               
                 Trastuzumab 
                 117 
                 evqlvesggglvqpggslrlscaasgfnikdtyihwvrqapgkglewvariyptngytryads 
               
               
                 VH (D98E) 
                   
                 vkgrftisadtskntaylqmnslraedtavyycsrwggegfyamdywgqgtlvtyss 
               
               
                   
               
               
                 Trastuzumab 
                 115 
                 astkgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglysl 
               
               
                 CH1 
                   
                 ssvvtypssslgtqtyicnvnhkpsntkvdkkvepkscdkth 
               
               
                   
               
               
                 Trastuzumab 
                 118 
                 diqmtqspsslsasvgdrvtitcrasqdvnvavawyqqkpgkapklliysasflysgvpsrfsg 
               
               
                 VL (T31V) 
                   
                 srsgtdftltisslqpedfatyycqqhyttpptfgqgtkveikr 
               
               
                   
               
               
                 Trastuzumab 
                 116 
                 tvaapsvfifppsdeqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskds 
               
               
                 CL 
                   
                 tyslsstltlskadyekhkvyacevthqglsspvtksfnrgec 
               
               
                   
               
               
                 Trastuzumab 
                 20 
                 gfnikdtyih 
               
               
                 VH CDR1 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 29 
                 riyptngytryadsvkg 
               
               
                 VH CDR2 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 79 
                 wggegfyamdy 
               
               
                 VH CDR3 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 104 
                 rasqdvnvava 
               
               
                 VL CDR1 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 18 
                 sasflys 
               
               
                 VL CDR2 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 19 
                 qqhyttppt 
               
               
                 VL CDR3 
                   
                   
               
               
                   
               
            
           
           
               
            
               
                 CrossMab-CDRG Her2GlyMab 
               
            
           
           
               
               
               
            
               
                 XPertuzumab 
                 109 
                 evqlvesggglvqpggslrlscaasgftftdytmdwvrqapgkglewvadvnpnsggsiyn 
               
               
                 heavy chain 
                   
                 qrfkgrftlsydrskntlylqmnslraedtavyycarnlgpsfyfdywgqgtlvtvssasvaaps 
               
               
                 (VHCL) 
                   
                 vfifppsdeqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsst 
               
               
                   
                   
                 ltlskadyekhkvyacevthqglsspvtksfnrgecdkthtcppcpapellggpsvflfppkpk 
               
               
                   
                   
                 dtlmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlh 
               
               
                   
                   
                 qdwlngkeykckvsnkalpapiektiskakgqprepqvytlpperdeltknqvslwclvkgf 
               
               
                   
                   
                 ypsdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealh 
               
               
                   
                   
                 nhytqkslslspgk 
               
               
                   
               
               
                 Pertuzumab 
                 110 
                 diqmtqspsslsasvgdrvtitckasqdvsigvawyqqkpgkapklliysasyrytgvpsrfsg 
               
               
                 light chain 
                   
                 sgsgtdftltisslqpedfatyycqqyyiypytfgqgtkveikssastkgpsvfplapsskstsggt 
               
               
                 (VLCH1) 
                   
                 aalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvpssslgtqtyicnvnh 
               
               
                   
                   
                 kpsntkvdkkvepksc 
               
               
                   
               
               
                 Trastuzumab 
                 111 
                 qvqlvqsgaevkkpgasvkvsckasgfnikdtyihwvrqapgqglewmgriyptngytrya 
               
               
                 CDRG heavy 
                   
                 qkfqgrvtmtrdtsistaymelsrlrsddtavyycsrwggegfyamdywgqgtmvtvssast 
               
               
                 chain 
                   
                 kgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssv 
               
               
                 (VHCH1) 
                   
                 vtvpssslgtqtyicnvnhkpsntkvdkkvepkscdkthtcppcpapellggpsvflfppkpk 
               
               
                   
                   
                 dtlmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlh 
               
               
                   
                   
                 qdwlngkeykckvsnkalpapiektiskakgqprepqvctlppsrdeltknqvslscavkgfy 
               
               
                   
                   
                 psdiavewesngqpennykttppvldsdgsfflvskltvdksrwqqgnvfscsvmhealhn 
               
               
                   
                   
                 hytqkslslspgk 
               
               
                   
               
               
                 Trastuzumab 
                 112 
                 diqltqppsvsvapgqtaritcgasqdvstavawyqqkpgqapvlvvysasflysgipsrfsgs 
               
               
                 CDRG light 
                   
                 rsgtdftltisrveagdeadyycqqhyttpptfgtgtkvtvlrtvaapsvfifppsdeqlksgtasv 
               
               
                 chain (VLCL) 
                   
                 vcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyace 
               
               
                   
                   
                 vthqglsspvtksfnrgec 
               
               
                   
               
               
                 Trastuzumab 
                 105 
                 evqlvqsgaevkkpgasvkvsckasgfnikdtyihwvrqapgqglewmgriyptngytrya 
               
               
                 CDRG VH 
                   
                 qkfqgrvtmtrdtsistaymelsrlrsddtavyycsrwggegfyamdywgqgtmvtvss 
               
               
                 (D98E, 
                   
                   
               
               
                 CDRG) 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 115 
                 astkgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglysl 
               
               
                 CH1 
                   
                 ssvvtvpssslgtqtyicnvnhkpsntkvdkkvepkscdkth 
               
               
                   
               
               
                 Trastuzumab 
                 106 
                 diqltqppsvsvapgqtaritcgasqdvstavawyqqkpgqapvlvvysasflysgipsrfsgs 
               
               
                 CDRG VL 
                   
                 rsgtdftltisrveagdeadyycqqhyttpptfgtgtkvtvlr 
               
               
                 (N30T, 
                   
                   
               
               
                 CDRG) 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 116 
                 tvaapsvfifppsdeqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskds 
               
               
                 CL 
                   
                 tyslsstltlskadyekhkvyacevthqglsspvtksfnrgec 
               
               
                   
               
               
                 Trastuzumab 
                 20 
                 gfnikdtyih 
               
               
                 CDRG VH 
                   
                   
               
               
                 CDR1 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 108 
                 riyptngytryaqkfqg 
               
               
                 CDRG VH 
                   
                   
               
               
                 CDR2 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 79 
                 wggegfyamdy 
               
               
                 CDRG VH 
                   
                   
               
               
                 CDR3 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 107 
                 gasqdvstava 
               
               
                 CDRG VL 
                   
                   
               
               
                 CDR1 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 18 
                 sasflys 
               
               
                 CDRG VL 
                   
                   
               
               
                 CDR2 
                   
                   
               
               
                   
               
               
                 Trastuzumab 
                 19 
                 qqhyttppt 
               
               
                 CDRG VL 
                   
                   
               
               
                 CDR3 
               
               
                   
               
               
                 Pertuzumab sequences in CrossMab-CDRG Her2GlyMab, CrossMab-XTra Her2GlyMab and CrossMab-XPer Her2GlyMab: see Pertuzumab wt (parent) sequences Table 32 
               
            
           
         
       
     
     Purification of Bispecific Antibodies 
     Bispecific antibodies were purified from cell culture supernatants by affinity chromatography using MabSelectSure-Sepharose™ (GE Healthcare, Sweden) and Superdex 200 size exclusion (GE Healthcare, Sweden) chromatography. Briefly, sterile filtered cell culture supernatants were captured on a MabSelect SuRe resin equilibrated with PBS buffer (10 mM Na 2 HPO 4 , 1 mM KH 2 PO 4 , 137 mM NaCl and 2.7 mM KCl, pH 7.4), washed with equilibration buffer and eluted with 25 mM sodium citrate at pH 3.0. The eluted protein fractions were pooled, neutralized with 2M Tris, pH 9.0 and further purified by size exclusion chromatography using a Superdex 200 26/60 GL (GE Healthcare, Sweden) column equilibrated with 20 mM histidine, 140 mM NaCl, pH 6.0. Size exclusion chromatography fractions were analysed by CE-SDS (Caliper Life Science, USA) and bispecific antibody containing fractions were pooled and stored at −80° C. 
     Analysis of Purified Proteins 
     The protein concentration of purified protein samples was determined by measuring the optical density (OD) at 280 nm, using the molar extinction coefficient calculated on the basis of the amino acid sequence. Purity, antibody integrity and molecular weight of bispecific and control antibodies were analyzed by CE-SDS using microfluidic Labchip technology (Caliper Life Science, USA). 5 μl of protein solution was prepared for CE-SDS analysis using the HT Protein Express Reagent Kit according manufacturer&#39;s instructions and analysed on LabChip GXII system using a HT Protein Express Chip. Data were analyzed using LabChip GX Software version 3.0.618.0. The aggregate content of bispecific and control antibody samples was analyzed by high-performance SEC using a Superdex 200 analytical size-exclusion column (GE Healthcare, Sweden) in 200 mM KH 2 PO 4 , 250 mM KCl, pH 7.0 running buffer at 25° C. 25 μg protein were injected on the column at a flow rate of 0.5 ml/min and eluted isocratic over 50 minutes. 
     Mass Spectrometry 
     The integrity of the amino acid backbone of reduced bispecific antibody light and heavy chains was verified by NanoElectrospray Q-TOF mass spectrometry after removal of N-glycans by enzymatic treatment with Peptide-N-Glycosidase F (Roche Molecular Biochemicals). The amount of heavy and light chain mis-pairing was also quantified. 
     Surface Plasmon Resonance 
     Instrument: BIAacore T100 (GE Healthcare) 
     
         
         
           
             Software: Biacore T100 Control, Version 2.02/2.03
           Biacore T100 Evaluation, Version 2.02/2.03   Biacore B3000 (Biacore)   Software: Biacore B3000 Control, Version 4.1.2
               BIAEvaluation, Version 4.1.1   
               
         
           
         
       
    
     Assayformat Chip: CM5-Chip 
     Kinetic constants and resulting affinities of &lt;Her2GlyMab&gt;-molecules were measured for both “Trastuzumab”- and “Pertuzumab”-functionalities respecitively. These two functionalities were distinguished via pre-complexation of Her2 with either amine coupled parental MAb “Trastuzumab” (FC1/2) or “Pertuzumab” FC3/4). Complex formation of parental MAb and Her2 commensed after injection of Her2 ECD. 
     As a consequence of pre-complexed parental MAb “Trastuzumab”/Her2 all “Trastuzumab”-binding sites are saturated, but all “Pertuzumab”-binding sites are available and vice versa. Finally the binding of the “&lt;Her2GlyMab&gt;”-molecules to be analyzed was measured via injections using increasing concentrations with each cycle. Association and dissociation observed were calculated with a Langmuir 1:1 binding model. To minimize dissociation of Her2 during the measurements, the kinetic constants were measured at T=25° C. 
     Amine Coupling of Capture Molecules 
     Standard amine coupling on flow cells 1 to 4 according to the manufacturer&#39;s instructions: CM5 Chip, T=25° C., running buffer: HBS-N buffer, activation by mixture of EDC/NHS, aimed at 800 RU; the parental Abs “Trastuzumab” or “Pertuzumab” were diluted in coupling buffer sodium acetate, pH 4.5, c=2-3 μg/mL; finally remaining activated carboxyl groups were blocked by injection of 1 M Ethanolamine. 
     Chip surface on flow cell 1 and 3 (with either amine coupled parental mAb “Trastuzumab” or “Pertuzumab”) were used as reference control surface for correction of possible buffer-effects or non-specific binding. 
     Kinetic Characterization of &lt;Her2GlyMab&gt; Molecules at 25° C. 
     Running buffer: PBS 
     All samples were diluted with running buffer+1 mg/mL BSA. 
     Capturing of HER2 ECD on flow cells 2 and 4: c=100 nM, flow 5 μl/min, time 120 sec. 
     Analyte samples: A classical concentration series of the &lt;Her2GlyMab&gt;-molecules were analyzed at five concentrations (c=300, 100, 33.33, 11.11 and 3.7 nM.) at a flow rate of 50 μl/min was injected. Singles for each concentration, one as a duplicate; association time: 180 sec., dissociation time: 900 sec. 
     Final regeneration was performed after each cycle using 10 mM Glycin pH 2.5 for amine coupled Mab “Trastuzumab” and 25 mM NaOH for amine coupled Mab “Pertuzumab”, contact time each 60 sec, flow rate 30 μl/min. 
     Kinetic parameters were calculated by using double referencing (control reference: binding of analyte to Mabs Trastuzumab and Pertuzumab respectively; Flow Cell: 1 respectively 3), concentration “0” used as the blank. Calculations were performed with model ‘Langmuir binding 1:1, RI (refractive index)=0. 
     Results: Expression &amp; Purification Bispecific, Bivalent &lt;Her2GlyMab&gt; Antibody Molecules 
     According the procedures described in the materials and methods above, the bispecific, bivalent &lt;Her2GlyMab&gt; antibody molecules OAscFab1, OAscFab2, OAscFabPer1, OAscFabPer2, CrossMab-XPer, CrossMab-XTra and CrossMab-CDRG were expressed and purified. In each molecule the VH and VL of part are based on optimized Trastuzumab sequences with mutations T31V or N30T in the variable light chain and mutation D98E in the heavy chain and the Pertuzumab parent sequence respectively. The schematic structure of the antibodies is shown in  FIGS. 10A and 10B . The sequences are shown in Table 8 above. 
     Expression of &lt;Her2GlyMab&gt; antibody molecules OAscFab1 Her2 GlyMab, OAscFab2 Her2 GlyMab, OAscFabPer1 Her2 GlyMab, OAscFabPer2 Her2 GlyMab (all glycoengineered, Knobs-into-holes, with the Trastuzumab scFab bearing D98E and T31V mutations), CrossMab-XPer Her2 GlyMab, CrossMab-XTra Her2 GlyMab (both glycoengineered, Knobs-into-holes, with the Trastuzumab cross-Fab bearing D98E and T31V mutations), and CrossMab-CDRG Her2 GlyMab (glycoengineered, Knobs-into-holes, CDR grafted, with the Trastuzumab cross-Fab bearing D98E and N30S mutations) was confirmed by western blotting and HP-SEC ( FIGS. 11A and 11B ). Purification of OAscFab1, OAscFab2, OAscFabPer1, OAscFabPer2, CrossMab-XPer, CrossMab XTra and CrossMab-CDRG led to the yields shown in Table 9. All OAscFab constructs showed less than 90% monomer post purification, the reduced purity of these molecules could not be increased by optimization of the plasmid ratios in expression (data not shown). However, optimization of the plasmid chain ratios in expression proved to increase the monomeric fraction present post purification of the CrossMab antibodies as described below. 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Her2GlyMab Purification - Analysis of the percentage aggregation 
               
               
                 post protein A and SEC purification using HP-SEC and 
               
               
                 the respective protein yields calculated by UV spectroscopy 
               
               
                 A280 of the glycoengineered constructs. 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Purity 
                 Expression 
                 Purity 
                   
               
               
                   
                 post 
                 yield post 
                 post 
                 Expression 
               
               
                   
                 protein A 
                 protein A 
                 SEC 
                 yield post 
               
               
                 Name 
                 (%) 
                 (mg/L) 
                 (%) 
                 SEC (mg/L) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 OAscFab1 
                 48.4 
                 144 
                 90 
                 31.6 
               
               
                 (SEQ ID NOs 
               
               
                 133, 134, 135) 
               
               
                 OAscFab2 
                 41 
                 42.9 
                 93.6 
                 12.6 
               
               
                 (SEQ ID NOs 
               
               
                 136, 137, 138) 
               
               
                 OAscFabPer1 
                 41.6 
                 43.4 
                 88.8 
                 4.6 
               
               
                 (SEQ ID NOs 
               
               
                 139, 140, 141) 
               
               
                 OAscFabPer2 
                 38.2 
                 34.2 
                 86.7 
                 6.6 
               
               
                 (SEQ ID NOs 
               
               
                 142, 143, 144) 
               
               
                 CrossMab-XTra 
                 65.1 
                 28.4 
                 73 
                 10.6 
               
               
                 (SEQ ID NOs 
               
               
                 119, 120, 121, 
               
               
                 122) 
               
               
                 CrossMab-XPer 
                 76.4 
                 30.9 
                 85 
                 17.9 
               
               
                 (SEQ ID NOs 
               
               
                 109, 110, 96, 86) 
               
               
                 CrossMab- 
                 73 
                 31.5 
                 95 
                 11.9 
               
               
                 CDRG 
               
               
                 (SEQ ID NOs 
               
               
                 109, 110, 111, 
               
               
                 112) 
               
               
                   
               
            
           
         
       
     
     Example 7: Expression &amp; Purification Bispecific, Bivalent &lt;Her2GlyMab&gt; Antibody Molecules Optimization of the Plasmid Ratios Used in Expression 
     CrossMab-XTra 
     According the procedures described in the materials and methods above, the bispecific, bivalent &lt;Her2GlyMab&gt; antibody molecule CrossMab-XTra, was expressed with molar plasmid ratios of 1:1:1:1, 1:1:1:2, 1:1:1:4 and 1:1:1:8 and purified. Expression of CrossMab-XTra was confirmed by Western blot. After Protein A purification of cell culture supernatants the construct showed at a 1:1:1:1 equimolar plasmid ratio approximately 73% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS; 11% containing 2× Pertuzumab light chains paired with Pertuzumab and XTrastuzumab heavy chains; 9% intact XTrastuzumab antibodies (both heavy and light chains originating from XTrastuzumab) with the formation of the heavy chain hole-hole association; 4% XTrastuzumab heavy chain hole-hole association combined with Pertuzumab light chains only; and 3% XTrastuzumab heavy chain hole-hole association with 1× XTrastuzumab light chain and 1× Pertuzumab light chain. 
     The 1:1:1:2 plasmid ratio where the molar ratio of the crossed Trastuzumab light chain (XHerLC) was expressed 2-fold, showed approximately 81% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS; 1% containing 2× Pertuzumab light chains paired with Pertuzumab and XTrastuzumab heavy chains; 16% intact XTrastuzumab antibodies (both heavy and light chains originating from XTrastuzumab) with the formation of the heavy chain hole-hole association; XTrastuzumab heavy chain hole-hole association combined with Pertuzumab light chains only were not detected; and 1% XTrastuzumab heavy chain hole-hole association with 1× XTrastuzumab light chain and 1× Pertuzumab light chain. 
     The 1:1:1:4 plasmid ratio where the molar ratio of the crossed Trastuzumab light chain was expressed 4-fold, showed approximately 64% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS; 2× Pertuzumab light chains paired with Pertuzumab and XTrastuzumab heavy chains was not detected, however, 12% 2× XTrastuzumab light chains paired with Pertuzumab and XTrastuzumab heavy chains was detected for the first time at this ratio; 24% intact XTrastuzumab antibodies (both heavy and light chains originating from XTrastuzumab) with the formation of the heavy chain hole-hole association; XTrastuzumab heavy chain hole-hole association combined with Pertuzumab light chains only were not detected; XTrastuzumab heavy chain hole-hole association with 1× XTrastuzumab light chain and 1× Pertuzumab light chain were not detected. 
     The 1:1:1:8 plasmid ratio where the molar ratio of the crossed Trastuzumab light chain was expressed 8-fold, showed approximately 45% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS; 2× Pertuzumab light chains paired with Pertuzumab and XTrastuzumab heavy chains was not detected, however, 28% 2× XTrastuzumab light chains paired with Pertuzumab and XTrastuzumab heavy chains was detected for the first time at this ratio; 27% intact XTrastuzumab antibodies (both heavy and light chains originating from XTrastuzumab) with the formation of the heavy chain hole-hole association; XTrastuzumab heavy chain hole-hole association combined with Pertuzumab light chains only were not detected; XTrastuzumab heavy chain hole-hole association with 1× XTrastuzumab light chain and 1× Pertuzumab light chain were not detected. 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 CrossMab-XTra Her2GlyMab - Q-TOF analysis and quantification 
               
               
                 of the by-product profile of the Her2GlyMab antibody 
               
               
                 constructs comparing the optimization plasmid titration 
               
               
                 of the crossed Trastuzumab light chain (XHer LC). 
               
            
           
           
               
               
               
               
               
            
               
                   
                 CrossMab- 
                 CrossMab- 
                 CrossMab- 
                 CrossMab- 
               
               
                 Detected 
                 XTra 
                 XTra 
                 XTra 
                 XTra 
               
               
                 protein species 
                 Her2GlyMab 
                 Her2GlyMab 
                 Her2GlyMab 
                 Her2GlyMab 
               
               
                 in MS 
                 1:1:1:1 
                 1:1:1:2 
                 1:1:1:4 
                 1:1:1:8 
               
               
                   
               
               
                 1 x XHer HC; 
                 N.D. 
                 N.D. 
                 ~12% 
                 ~28% 
               
               
                 1 x Per HC; 
               
               
                 2 x XHer LC 
               
               
                 2 x XHer HC; 
                 ~9% 
                 ~16%  
                 ~24% 
                 ~27% 
               
               
                 2 x XHer LC 
               
               
                 CrossMab- 
                 ~73%  
                 ~81%  
                 ~64% 
                 ~45% 
               
               
                 XTra 
               
               
                 Her2GlyMab 
               
               
                 (100%) 
               
               
                 2 x XHer HC; 
                 ~3% 
                 ~1% 
                 N.D. 
                 N.D. 
               
               
                 1 x XHer LC; 
               
               
                 1 x Per HC 
               
               
                 1 x XHer HC; 
                 ~11%  
                 ~1% 
                 N.D. 
                 N.D. 
               
               
                 1 x Per HC; 
               
               
                 2 x Per LC 
               
               
                 2 x XHer HC; 
                 ~4% 
                 N.D. 
                 N.D. 
                 N.D. 
               
               
                 2 x Per LC 
               
               
                   
               
               
                 N.D.—Not Detected 
               
            
           
         
       
     
     CrossMab-XPer 
     After Protein A purification of cell culture supernatants the construct showed at a 1:1:1:1 equimolar plasmid ratio approximately 85% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS; 2% containing 2× XPertuzumab light chains paired with XPertuzumab and Trastuzumab heavy chains; 1% intact Trastuzumab antibodies (both heavy and light chains originating from Trastuzumab) with the formation of the heavy chain hole-hole association; 12% 2× Trastuzumab light chains paired with XPertuzumab and Trastuzumab heavy chains. No further species were detected. 
     The 1:1:1:2 plasmid ratio where the molar ratio of the crossed Pertuzumab light chain (XPerLC) was expressed 2-fold, showed approximately 89% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS; 7% containing 2× XPertuzumab light chains paired with XPertuzumab and Trastuzumab heavy chains; intact Trastuzumab antibodies (both heavy and light chains originating from Trastuzumab) with the formation of the heavy chain hole-hole association were not detected; 4% 2× Trastuzumab light chains paired with XPertuzumab and Trastuzumab heavy chains. 
     The 1:1:1:4 plasmid ratio where the molar ratio of the crossed Pertuzumab light chain (XPerLC) was expressed 4-fold, showed approximately 74% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS; 25% containing 2× XPertuzumab light chains paired with XPertuzumab and Trastuzumab heavy chains; intact Trastuzumab antibodies (both heavy and light chains originating from Trastuzumab) with the formation of the heavy chain hole-hole association were not detected; 1% 2× Trastuzumab light chains paired with XPertuzumab and Trastuzumab heavy chains. 
     The 1:1:1:8 plasmid ratio where the molar ratio of the crossed Pertuzumab light chain (XPerLC) was expressed 8-fold, showed approximately 52% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS; 48% containing 2× XPertuzumab light chains paired with XPertuzumab and Trastuzumab heavy chains; intact Trastuzumab antibodies (both heavy and light chains originating from Trastuzumab) with the formation of the heavy chain hole-hole association were not detected; 2× Trastuzumab light chains paired with XPertuzumab and Trastuzumab heavy chains were not detected. 
     CrossMab-CDRG 
     After Protein A purification of cell culture supernatants the construct showed at a 1:1:1:1 equimolar plasmid ratio approximately 95% of bispecific antibody with the expected molecular weight of approximately 148 kDa as detected by Q-TOF MS and 5% containing 2× XPertuzumab light chains paired with XPertuzumab and Trastuzumab (HerCDRG) heavy chains. 
     No further species were detected, therefore, further optimization of the plamid ratios was not performed. The MS spectra performed on the antibodies CrossMab-XHer and CrossMab-CDRG to compare the byproduct profile can be seen in  FIG. 12 . 
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 CrossMab-XPer Her2GlyMab - Q-TOF analysis and quantification 
               
               
                 of the by-product profile of the Her2GlyMab antibody 
               
               
                 constructs comparing the optimization plasmidtitration 
               
               
                 of the crossed Trastuzumab light chain (XPer LC). 
               
            
           
           
               
               
               
               
               
            
               
                 Detected 
                 CrossMab- 
                 CrossMab- 
                 CrossMab- 
                 CrossMab- 
               
               
                 protein 
                 XPer 
                 XPer 
                 XPer 
                 XPer 
               
               
                 species 
                 Her2GlyMab 
                 Her2GlyMab 
                 Her2GlyMab 
                 Her2GlyMab 
               
               
                 in MS 
                 1:1:1:1 
                 1:1:1:2 
                 1:1:1:4 
                 1:1:1:8 
               
               
                   
               
               
                 1 x Her HC; 
                 ~2% 
                 ~7% 
                 ~25% 
                 ~48% 
               
               
                 1 x XPer HC; 
               
               
                 2 x XPer LC 
               
               
                 2 x Her HC; 
                 ~1% 
                 n.d. 
                 n.d. 
                 n.d. 
               
               
                 2 x Her LC 
               
               
                 CrossMab- 
                 ~85% 
                 ~89%  
                 ~74% 
                 ~52% 
               
               
                 XPer 
               
               
                 Her2GlyMab 
               
               
                 (100%) 
               
               
                 1 x Her HC; 
                 ~12% 
                 ~4% 
                  ~1% 
                 n.d. 
               
               
                 1 x XPer HC; 
               
               
                 2 x Her LC 
               
               
                   
               
               
                 N.D.—Not Detected. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 CrossMab-CDRG Her2GlyMab - Q-TOF analysis and quantification of 
               
               
                 the by-product profile of the Her2GlyMab antibody construct detecting 
               
               
                 the presence of mispaired antibody heavy and light chains. 
               
            
           
           
               
               
               
            
               
                   
                   
                 CrossMab- 
               
               
                   
                   
                 CDRG 
               
               
                   
                   
                 Her2GlyMab 
               
               
                   
                 Detected protein species in MS 
                 1:1:1:1 
               
               
                   
                   
               
               
                   
                 2 x XPer HC; 2 x XPer LC 
                 N.D. 
               
               
                   
                 2 x HerCDRG HC; 2 x HerCDRG LC 
                 N.D. 
               
               
                   
                 CrossMab-CDRG Her2GlyMab (100%) 
                 ~95%  
               
               
                   
                 1 x HerCDRG HC; 1 x XPer HC; 2 x XPer LC 
                 ~5% 
               
               
                   
                   
               
               
                   
                 N.D.—Not Detected 
               
            
           
         
       
     
     Example 8: Simultaneous Binding of Bispecific Antibodies to Both Antigens 
     The binding of the bispecific antibody was analyzed via BIAcore as described above. 
     In separate assay format samples (CrossMabXPer as well as for OAscFab1 and OAscFab2) were proven to be functional for Trastuzumab as well as Pertuzumab specificity. CrossMabXPer showed kinetic constants and resulting affinities in the same order of magnitude as the positive controls. Except for a slightly reduced k a -rate constant for the Trastuzumab mediated binding OAscFab1 and OAscFab2 showed kinetic constants and resulting affinities in the same order of magnitude as the positive controls i.e the parental Mabs. 
     Partly bivalent binding of the positive controls-depending on the ligand density on the CM5-Chip may cause the variation of the dissociation rate constants in the two experiments depicted in this example. 
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 SPR analysis of the Her2GlyMab affinities - The association and dissociation rates 
               
               
                 of the antibodies were measure using a BIAcoreT100 with a CM5-Chip at 25° C. 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 T = 25° C. 
                   
                   
                   
                   
                   
               
               
                   
                 analyzed 
                   
                 k a   
                 k d   
                 t(½) 
                 K D   
               
               
                 Experiment 
                 function 
                 analyte 
                 [M −1  · s −1 ] 
                 [s −1 ] 
                 [min] 
                 [M] 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 UJ2530 
                 “Trastuzumab” 
                 Trastuzumab 
                 3.9E+05 
                 8.7E−05 
                 132.5 
                 2.2E−10 
               
            
           
           
               
               
               
               
            
               
                 UJ2530 
                 “Trastuzumab” 
                 Pertuzumab 
                 no binding as expected 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 UJ2530 
                 “Trastuzumab” 
                 CrossMabXPer 
                 1.0E+05 
                 7.6E−05 
                 151.9 
                 7.4E−10 
               
            
           
           
               
               
               
               
            
               
                 UJ2530 
                 “Pertuzumab” 
                 Trastuzumab 
                 no binding as expected 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 UJ2530 
                 “Pertuzumab” 
                 Pertuzumab 
                 4.7E+05 
                 9.8E−05 
                 118.1 
                 2.1E−10 
               
               
                 UJ2530 
                 “Pertuzumab” 
                 CrossMabXPer 
                 2.0E+05 
                 1.8E−04 
                 66.0 
                 8.7E−10 
               
               
                 UJ2530_b 
                 “Trastuzumab” 
                 Trastuzumab 
                 7.0E+05 
                 3.0E−05 
                 382.9 
                 4.3E−11 
               
            
           
           
               
               
               
               
            
               
                 UJ2530_b 
                 “Trastuzumab” 
                 Pertuzumab 
                 no binding as expected 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 UJ2530_b 
                 “Trastuzumab” 
                 OAscFab1 
                 1.6E+04 
                 8.7E−05 
                 133.5 
                 5.4E−09 
               
               
                 UJ2530_b 
                 “Trastuzumab” 
                 OAscFab2 
                 1.8E+04 
                 1.1E−04 
                 100.6 
                 6.2E−09 
               
            
           
           
               
               
               
               
            
               
                 UJ2530_b 
                 “Pertuzumab” 
                 Trastuzumab 
                 no binding as expected 
               
            
           
           
               
               
               
               
               
               
               
            
               
                 UJ2530_b 
                 “Pertuzumab” 
                 Pertuzumab 
                 2.4E+05 
                 2.6E−04 
                 44.8 
                 1.1E−09 
               
               
                 UJ2530_b 
                 “Pertuzumab” 
                 OAscFab1 
                 1.2E+05 
                 2.6E−04 
                 44.3 
                 2.2E−09 
               
               
                 UJ2530_b 
                 “Pertuzumab” 
                 OAscFab2 
                 1.7E+05 
                 2.6E−04 
                 44.8 
                 1.5E−09 
               
               
                   
               
            
           
         
       
     
     Example 9: In Vitro Evaluation of 1+1 Herceptarg CrossMAb and Glyocoengineered Herceptarg Crossmab 
     Proliferation Inhibition Assay 
     AlamarBlue® (Invitrogen) was used for the measurement of the metabolic activity and proliferation of (A) BT474 and (B) N87 cells after a 5 day incubation in presence of HER2 CrossMab (CrossMab-XTra Her2GlyMab, SEQ ID NOs 119, 120, 121, 122), Trastuzumab, Pertuzumab or the combination of Trastuzumab/Pertuzumab. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). 
     Target cells were harvested, washed, resuspended in RPMI 1640 (Gibco)+10% FCS+1% GlutaMAX™ (Gibco) and plated at a concentration of 1×10 4  cells/well. Cells were incubated for 3 hours in the cell incubator before respective antibody dilutions were added. Plates were gently shaked and incubated for 5 days in the cell incubator. 
     25 μl/well of Alamar Blue were added to the plate and incubated for 7 h in the incubator. 
     Absorbance was monitored at 584 nm and 612 nm in a Wallac Victor3 1420 Multilabel Counter. 
     For calculation of the percentage of proliferation inhibition, non-treated controls samples were included in the assay and defined as 100% proliferation. The average percentage of proliferation inhibition of the triplicates of each experiment was calculated. 
     Results are shown in  FIGS. 13A and 13B . 
     ADCC Assay 
     ADCC mediated by HER2 CrossMab (CrossMab-XTra Her2GlyMab, SEQ ID NOs 119, 120, 121, 122), Trastuzumab, Pertuzumab or the combo of Trastuzumab/Pertuzumab was assessed on KPL-4 (A), T47D (B) and Calu-3 (C) cells. 
     Target cells were harvested, washed, resuspended in AIM V® medium (Life Technologies), and plated at a concentration of 3×10 4  cells/well. The respective antibody dilutions were added in triplicates to the cells and incubated for 10 min before addition of the effector cells (peripheral blood mononuclear effector cells [PBMCs]). Effector (E) and target (T) cells were then incubated for 4 h at 37° C. at an E:T ratio of 25:1 (triplicates for all samples). Lactate dehydrogenase (LDH) release was measured using the LDH Cytotoxicity Detection Kit (Roche Applied Science). ADCC was calculated using the following formula: 
     
       
         
           
             
               Percentage 
                
               
                   
               
                
               ADCC 
             
             = 
             
               
                 ( 
                 
                   [ 
                   
                     
                       
                         sample 
                          
                         
                             
                         
                          
                         release 
                       
                       - 
                       
                         spontaneous 
                          
                         
                             
                         
                          
                         release 
                       
                     
                     
                       
                         maximal 
                          
                         
                             
                         
                          
                         release 
                       
                       - 
                       
                         spontaneous 
                          
                         
                             
                         
                          
                         release 
                       
                     
                   
                   ] 
                 
                 ) 
               
               × 
               100. 
             
           
         
       
     
     Spontaneous release, corresponding to target cells incubated with effector cells without antibody, was defined as 0% cytotoxicity, with maximal release (target cells lysed with 1% Triton X-100) defined as 100% cytotoxicity. The average percentage of ADCC and standard deviations of the triplicates of each experiment were calculated. Results are shown in  FIGS. 14A-14C . 
     Example 10: In Vivo Characterization of HER2 CrossMab: Effect of Bispecific Antibodies Targeting HER2 on Tumor Growth in Calu3 Lung Cancer and KPL4 Breast Cancer Xenograft 
     In Vitro Cultured Cells—Calu3 
     This human lung adenocarcinoma cancer cell line has been established from a human caucasian male with lung cancer. Cells were obtained from Chugai Pharmaceuticals Co., Ltd. and passaged in house for working cell bank. Tumor cells are routinely cultured in RPMI medium (PAN Biotech, Germany) supplemented with 10% fetal bovine serum glutamine (PAN Biotech, Germany) at 37° C. in a water-saturated atmosphere at 5% CO2. Culture passage is performed with trypsin/EDTA 1× (PAN) splitting twice/week. Cell passage P6 is used for in vivo study. 
     In Vitro Cultured Cells—KPL-4 
     This human breast cancer cell line has been established from the malignant pleural effusion of a breast cancer patient with an inflammatory skin metastasis. Cells have been provided by Professor J. Kurebayashi (Kawasaki Medical School, Kurashiki, Japan). Tumor cells are routinely cultured in DMEM medium (PAN Biotech, Germany) supplemented with 10% fetal bovine serum (PAN Biotech, Germany) and 2 mM L-glutamine (PAN Biotech, Germany) at 37° C. in a water-saturated atmosphere at 5% CO2. Culture passage is performed with trypsin/EDTA 1× (PAN) splitting twice/week. Cell passage P6 is used for in vivo study. 
     Animals 
     Female SCID beige (C.B.-17) mice; age 10-12 weeks; body weight 18-20 g (Charles River Germany, Sulzfeld) or female BALB/C nu/nu mice; age 8-10 weeks; body weight &gt;20 g (Bomholtgard, Denmark) are maintained under specific-pathogen-free condition with daily cycles of 12 h light/12 h darkness according to international guidelines (GV-Solas; Felasa; TierschG). After arrival animals are housed in the quarantine part of the animal facility for one week to get accustomed to new environment and for observation. Continuous health monitoring is carried out on regular basis. Diet food (Alltromin) and water (acidified pH 2.5-3) are provided ad libitum. The experimental study was reviewed and approved by local government; registration no. 55.2-1-54-2531.2-3-08 Scid-beige orthotop rodent breast cancer model and 211-2531.2-16/00. 1.2.2 subcutan tumor model. 
     Tumor Cell Injection 
     At the day of injection tumor cells are harvested (trypsin-EDTA) from culture flasks (Greiner TriFlask) and transferred into 50 ml culture medium, washed once and resuspended in PBS. After an additional washing step with PBS and filtration (cell strainer; Falcon Ø 100 μm) the final cell titer is adjusted to 1.5×108/ml. Tumor cell suspension is carefully mixed with transfer pipette to avoid cell aggregation. Anesthesia is performed using a Stephens inhalation unit for small animals with preincubation chamber (plexiglas), individual mouse nose-mask (silicon) and not flammable or explosive anesthesia compound Isoflurane (Pharmacia-Upjohn, Germany) in a closed circulation system. Two days before injection, coat of the SCID beige mice are shaved. For subcutaneous injection of Calu3 cells, skin of anaesthetized animals is carefully lifted up with an anatomic forceps and 100 μl cell suspension (=5.0×10e6 cells) is injected subcutaneously in the right flank of the animals. Cell suspension is filled into a 1.0 ml tuberculin syringe (Braun, Melsungen) using a wide injection needle (0.45×25 mm). KPL-4 cells (3×10e6 cells) are injected orthotopically in a volume of 20 μl into the right penultimate inguinal mammary fat pad of each anesthetized mouse. For the orthotopic implantation, the cell suspension is injected through the skin under the nipple using a using a Hamilton microliter syringe and a 30 G×½″ needle. 
     Monitoring 
     Animals are controlled daily for detection of clinical symptoms of adverse effects. For monitoring throughout the experiment the body weight of the animals is documented two times weekly and the tumor volume is measured by caliper twice weekly. Tumor volume was calculated according to NCI protocol (Tumor weight=½ab 2 , where “a” and “b” are the long and the short diameters of the tumor, respectively). Termination criteria were the critical tumor mass (up to 1.7 g or Ø&gt;1.5 cm), body weight loss more than 20% from baseline, tumor ulceration or poor general condition of the animals. Study exclusion criteria for the animals are described and approved in the corresponding “Tierversuchsanzeige”. 
     Treatment of Animals 
     Mice were randomized for tumor volume, for KPL-4 a mean of 80 mm 3 , for Calu3 a mean of 100 mm 3 . Mice were treated once weekly with a volume of 10 ml/kg intra peritoneal. For combination treatment Trastuzumab was given first and Pertuzumab was given 24 hrs thereafter. 
     Results are shown in tables 14 to 17 and  FIGS. 15A-15C to 18A-18C . 
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 BispecHer2_Pz_Calu3_001 (FIGS. 15A-15C): CrossMAb_003 non- 
               
               
                 ge: non glycoengineered CrossMab-XTra Her2GlyMab (SEQ ID NOs 119, 120, 
               
               
                 121, 122), negative control: anti-IgE antibody (Omalizumab), Herceptarg 
               
               
                 2 + 2 OmniE: Pertuzumab antibody with the Trastuzumab scFV added 
               
               
                 onto the c-terminus of the heavy chains. The trastuzumab scFv contains 
               
               
                 a stabilizing disulphide bond between VH 105-VL 43 (SEQ ID NOs: 145, 
               
               
                 146), TvAb12 and TvAb20: scFv 2 + 2 HER2 bispecific antibodies, see example 2. 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 Cumulative 
               
               
                   
                 No of 
                   
                 Dose 
                 Route/Mode of 
                 No of 
                 dose 
               
               
                 Group 
                 animals 
                 Compound 
                 (mg/kg) 
                 administration 
                 treatments 
                 (mg/kg) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 8 
                 negative control 
                 10 
                 i.p. once weekly 
                 7 
                 70 
               
               
                 2 
                 8 
                 Trastuzumab + 
                 10 
                 i.p. once weekly 
                 7 
                 70 
               
               
                   
                   
                 Pertuzumab 
                 10 
                 i.p. once weekly 
                 7 
                 70 
               
               
                 3 
                 8 
                 Herceptarg 2 + 2 
                 13.5 
                 i.p. once weekly 
                 7 
                 94.5 
               
               
                   
                   
                 OmniE 
               
               
                 4 
                 8 
                 CrossMAb_003 
                 20 
                 i.p. once weekly 
                 7 
                 140 
               
               
                   
                   
                 non-ge 
               
               
                 5 
                 8 
                 TvAb12 
                 11.3 
                 i.p. once weekly 
                 7 
                 79.1 
               
               
                 6 
                 8 
                 TvAb20 
                 11.4 
                 i.p. once weekly 
                 7 
                 79.8 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 BispecHER2_PZ_KPL-4_002 (FIGS. 16A-16C): CrossMAb_003 non- 
               
               
                 ge: non glycoengineered CrossMab-XTra Her2GlyMab (SEQ ID NOs 119, 120, 
               
               
                 121, 122), negative control: anti-IgE antibody (Omalizumab), Herceptarg 
               
               
                 2 + 2 OmniE: Pertuzumab antibody with the Trastuzumab scFV added 
               
               
                 onto the c-terminal of the heavy chains. The trastuzumab scFv contains 
               
               
                 a stabilizing disulphide bond between VH 105-VL 43 (SEQ ID NOs: 145, 
               
               
                 146), TvAb12 and TvAb20: scFv 2 + 2 HER2 bispecific antibodies, see example 2. 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 Cumulative 
               
               
                   
                 No of 
                   
                 Dose 
                 Route/Mode of 
                 No of 
                 dose 
               
               
                 Group 
                 animals 
                 Compound 
                 (mg/kg) 
                 administration 
                 treatments 
                 (mg/kg) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 9 
                 Negative control 
                 10 
                 i.p. once weekly 
                 5 
                 50 
               
               
                 2 
                 9 
                 Trastuzumab + 
                 10 
                 i.p. once weekly 
                 5 
                 50 
               
               
                   
                   
                 Pertuzumab 
                 10 
                 i.p. once weekly 
                   
                 50 
               
               
                 3 
                 9 
                 Herceptarg 2 + 2 
                 13.5 
                 i.p. once weekly 
                 5 
                 67.5 
               
               
                   
                   
                 OmniE 
               
               
                 4 
                 9 
                 CrossMAb_003 
                 20 
                 i.p. once weekly 
                 5 
                 100 
               
               
                   
                   
                 non-ge 
               
               
                 5 
                 9 
                 TvAb12 
                 11.3 
                 i.p. once weekly 
                 5 
                 56.5 
               
               
                 6 
                 9 
                 TvAb20 
                 11.42 
                 i.p. once weekly 
                 5 
                 57.1 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Bispec.HER2_PZ_KPL-4_003 (FIGS. 17A-17C): 
               
               
                 CrossMAb_005 non glycoengineered CrossMab-XTra Her2GlyMab 
               
               
                 (SEQ ID NOs 119, 120, 121, 122), negative control: anti-IgE antibody (Omalizumab). 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 Cumulative 
               
               
                   
                 No of 
                   
                 Dose 
                 Route/Mode of 
                 No of 
                 dose 
               
               
                 Group 
                 animals 
                 Compound 
                 (mg/kg) 
                 administration 
                 treatments 
                 (mg/kg) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 10 
                 Negative control 
                 10 
                 i.p. once weekly 
                 5 
                 50 
               
               
                 2 
                 10 
                 Trastuzumab + 
                 10 
                 i.p. once weekly 
                 5 
                 50 
               
               
                   
                   
                 Pertuzumab 
                 10 
                 i.p. once weekly 
                   
                 50 
               
               
                 3 
                 10 
                 Her2_Crossmab_005 
                 20 
                 i.p. once weekly 
                 5 
                 100 
               
               
                 4 
                 10 
                 Her2_Crossmab_005 
                 10 
                 i.p. once weekly 
                 5 
                 50 
               
               
                 5 
                 10 
                 Her2_Crossmab_005 
                 5 
                 i.p. once weekly 
                 5 
                 25 
               
               
                 6 
                 10 
                 Her2_Crossmab_005 
                 1 
                 i.p. once weekly 
                 5 
                 5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 Exploratory_PZ_KPL-4_009 (FIGs. 18A-18C): negative 
               
               
                 control: anti-IgE antibody (Omalizumab), TvAb16 and TvAb20: 
               
               
                 scFv 2 + 2 HER2 bispecific antibodies, see example 2. 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                 Cumulative 
               
               
                   
                 No of 
                   
                 Dose 
                 Route/Mode of 
                 No of 
                 dose 
               
               
                 Group 
                 animals 
                 Compound 
                 (mg/kg) 
                 administration 
                 treatments 
                 (mg/kg) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 10 
                 Negative 
                 10 
                 i.p. once weekly 
                 4 
                 40 
               
               
                   
                   
                 control 
               
               
                 2 
                 10 
                 Trastuzumab + 
                 10 
                 i.p. once weekly 
                 4 
                 40 
               
               
                   
                   
                 Pertuzumab 
                 10 
                 i.p. once weekly 
                 4 
                 40 
               
               
                 3 
                 10 
                 Trastuzumab + 
                 5 
                 i.p. once weekly 
                 4 
                 20 
               
               
                   
                   
                 Pertuzumab 
                 5 
                 i.p. once weekly 
                 4 
                 20 
               
               
                 4 
                 10 
                 TvAb16 
                 10 
                 i.p. once weekly 
                 4 
                 40 
               
               
                 5 
                 10 
                 TvAb16 
                 5 
                 i.p. once weekly 
                 4 
                 20 
               
               
                 6 
                 10 
                 TvAb20 
                 10 
                 i.p. once weekly 
                 4 
                 40 
               
               
                 7 
                 10 
                 TvAb20 
                 5 
                 i.p. once weekly 
                 4 
                 20 
               
               
                   
               
            
           
         
       
     
     Example 11: Generation of a Common Light Chain for Trastuzumab and Pertuzumab 
     Gene Synthesis 
     Desired gene segments, where required, were either generated by PCR using appropriate templates or were synthesized at Geneart AG (Regensburg, Germany) from synthetic oligonucleotides and PCR products by automated gene synthesis. In cases where no exact gene sequence was available, oligonucleotide primers were designed based on sequences from closest homologues and the genes were isolated by RT-PCR from RNA originating from the appropriate tissue. The gene segments flanked by singular restriction endonuclease cleavage sites were cloned into standard cloning/sequencing vectors. The plasmid DNA was purified from transformed bacteria and concentration determined by UV spectroscopy. The DNA sequence of the subcloned gene fragments was confirmed by DNA sequencing. Gene segments were designed with suitable restriction sites to allow subcloning into the respective expression vectors. All constructs were designed with a 5′-end DNA sequence coding for a leader peptide which targets proteins for secretion in eukaryotic cells. SEQ ID NOs: 155, 156, and 157 give exemplary leader peptides. 
     Cloning of Antigen Expression Vectors 
     A DNA fragment encoding amino acids 1 to 629 of matured Tyrosine kinase-type cell surface receptor HER2 (Her2, Uniprot: P04626) was cloned in frame into a mammalian recipient vector containing an N-terminal leader sequence. In addition, the construct contains a C-terminal avi-tag allowing specific biotinylation during co-expression with Bir A biotin ligase and a His-tag used for purification by immobilized-metal affinity chromatography (IMAC) (SEQ ID NOs 1 and 2). 
     The antigen expression is generally driven by an MPSV promoter and transcription is terminated by a synthetic polyA signal sequence located downstream of the CDS. In addition to the expression cassette, each vector contains an EBV oriP sequence for autonomous replication in EBV-EBNA expressing cell lines. 
     Production and Purification of Antigens and Antibodies 
     Both antigens and antibodies were transiently transfected into HEK 293 cells, stably expressing the EBV-derived protein EBNA. A simultaneously co-transfected plasmid encoding biotin ligase Bir A allowed avi tag-specific biotinlylation in vivo. The proteins were then purified using a protein A column followed by gel filtration. 
     Design of Trastuzumab/Pertuzumab Common Light Chains 
     For the generation of a common light chain (CLC) for Trastuzumab and Pertuzumab, the individual light chains (LC) were analyzed and compared. Sequence analysis revealed that both variable domains originate from the same germline sequence. Given that the Trastuzumab LCDR3 in general and residue H91 in particular interact specifically with the Trastuzumab-specific epitope on Her2, the first attempt to create a Trastuzumab/Pertuzumab CLC was done as follows: Either the complete LCDR3 region or only residue H91 of Trastuzumab substituted the corresponding positions in the Pertuzumab LC. As a result, a hybrid LC construct was created encoding Pertuzumab-derived LCDR1 and 2 but harboring Trastuzumab-derived LCDR3 amino acid residues. The resulting CLCs, named either “Pertuzumab (Tras.L3) LC” (DNA sequence of variable domains listed as SEQ ID NO: 25) or “Pertuzumab (Tras.Y91H) LC” (SEQ ID NO: 27), were co-expressed with either the Trastuzumab or the Pertuzumab HC. The resulting four antibodies (protein sequence of variable domains listed as SEQ ID NOs: 22 and 26, “Pertuzumab HC”×“Pertuzumab (Tras.L3) LC”; SEQ ID NO: 92 and 26, “Trastuzumab HC”×“Pertuzumab (Tras.L3) LC”; SEQ ID NOs: 22 and 28, “Pertuzumab HC”×“Pertuzumab (Trast.Y91H) LC”; SEQ ID NO: 92 and 28, “Trastuzumab HC”×“Pertuzumab (Tras.Y91H) LC”) were purified from mammalian-derived cell culture supernatant and binding to Her2 was measured and compared with the respectiv parental antibodies (SEQ ID NOs: 22 and 24, “Pertuzumab HC”×“Pertuzumab LC”; SEQ ID NO: 92 and 82, “Trastuzumab HC”×“Trastuzumab LC”) by SPR. 
     Affinity-Determination by SPR Using BioRad&#39;s ProteOn XPR36 Biosensor 
     The Affinity (K D ) of the new antibody chain combinations was measured by surface plasmon resonance using a ProteOn XPR36 instrument (Biorad) at 25° C. In a first step, 6500 RU of anti-human IgG (Sigma 12136, polyclonal goat antibody) recognizing hu IgG (Fc-specific) was immobilized on all 6 channels of a GLM chip by Amine coupling (NaAcetate pH4, 30 μl/min, 300 s) (vertical orientation). 
     Each antibody was diluted with PBST (10 mM phosphate, 150 mM sodium chloride pH 7.4, 0.005% Tween 20) to 2 μg/ml, and then injected for 60 s at 30 μl/minute to achieve immobilization levels of about 400 response units (RU) in vertical orientation. Injection of Her2: For one-shot kinetics measurements, injection direction was changed to horizontal orientation, three-fold dilution series of purified Her2 (varying concentration ranges between 300 and 3.7 nM) were injected simultaneously at 100 μl/min along separate channels 1-5, with association times of 180 s, and dissociation times of 600 s. Buffer (PBST) was injected along the sixth channel to provide an “in-line” blank for referencing. Regeneration was performed by two pulses of 10 mM glycine pH 1.5 and 50 mM NaOH for 30 s at 100 μl/min (horizontal orientation). Association rate constants (k on ) and dissociation rate constants (k off ) were calculated using a simple one-to-one Langmuir binding model in ProteOn Manager v3.1 software by simultaneously fitting the association and dissociation sensorgrams. The equilibrium dissociation constant (K D ) was calculated as the ratio k off /k on . 
     As expected, both control antibodies Trastuzumab and Pertuzumab recognized Her2 with their known affinities in the low nanomolar or sub-nanomolar range. However, while the affinity of Pertuzumab HC in combination with the newly designed “Pertuzumab (Tras.Y91H) LC” was slightly reduced, the same light chain in combination with Trastuzumab HC did not yield any detectable binding. This indicates that a single Trastuzumab-derived point mutation (Y91H) in the Pertuzumab LC is not sufficient translate binding to Her2 in this chain combination. This is in contrast to the second CLC variant “Pertuzumab LC (Trast. L3)” which resulted in weak binding when combined with Trastuzumab HC, while binding was was reduced but clearly visible when co-expressed with the Pertuzumab HC. A summary of the kinetic and thermodynamic measurements is given in  FIG. 19  and table 18. Based on this finding, the CLC “Pertuzumab LC (Trast. L3)” was further modified in order to restore Her2 binding in combination with Trastuzumab HC while affecting the affinity as little as possible when co-expressed with Pertuzumab HC. 
     
       
         
           
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                 Kinetic and Thermodynamic parameters of Trastuzumab, 
               
               
                 Pertuzumab, and hybrid molecules thereof 
               
            
           
           
               
               
               
               
            
               
                 Clone 
                 ka [1/Ms] 
                 kd [1/s] 
                 KD [M] 
               
               
                   
               
               
                 Trastuzumab 
                 1.01E+05 
                 2.24E−04 
                 2.21E−09 
               
               
                 Pertuzumab 
                 8.08E+04 
                 6.69E−05 
                 1.47E−10 
               
               
                 Pertuzumab HC 
                 6.47E+04 
                 1.73E−03 
                 2.67E−08 
               
               
                 Pertuzumab (Tras.L3) LC 
               
               
                 Pertuzumab HC 
                 8.99E+04 
                 1.98E−04 
                 2.21E−09 
               
               
                 Pertuzumab(Tras.Y91H) LC 
               
               
                 Trastuzumab HC 
                 4.19E+04 
                 0.05 
                 1.09E−06 
               
               
                 Pertuzumab(Tras.L3) LC 
               
               
                 Trastuzumab HC 
                 N/A 
                 N/A 
                 N/A 
               
               
                 Pertuzumab(Tras.Y91H) LC 
               
               
                   
               
            
           
         
       
     
     Example 12: Affinity-Improvement of the Common Light Chain 
     Introduction and Characterization of Additional Trastuzumab-Specific LCDR Residues into the Pertuzumab (Tras.L3) LC 
     Based on the results of the previous SPR measurement, binding of the designed CLC “Pertuzumab (Trast. L3) LC” in combination with the Pertuzumab HC led to a reduced but still well detectable binding. In contrast, co-expression of the CLC with Trastuzumab HC resulted in a very weak affinity in the micromolar range and was significantly reduced compared to the parental Trastuzumab antibody. 
     In order to further evolve the generation of a CLC and improve the binding in combination with the Trastuzumab HC, additional Trastuzumab-specific amino acid of the LCDR1, 2, and 3 as well in framework 3 region that are specific for Trastuzumab were individually introduced into the sequence of the previously designed “Pertuzumab (Trast.L3) LC” (DNA sequence of variable domains listed as SEQ ID NO: 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, and 51). The resulting constructs (protein sequence of variable domains listed as SEQ ID NO: 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, and 52) were co-expressed with the Trastuzumab HC (SEQ ID NO: 92) or the Pertuzumab HC (SEQ ID NO: 22) and purified from mammalian-derived cell culture supernatant. Binding to Her2 was measured and compared with the respective parental antibodies. 
     Affinity-Determination of the “Pertuzumab (Trast.L3) LC” Variants by SPR 
     The Affinity (K D ) of the new antibody chain combinations was measured by surface plasmon resonance using a ProteOn XPR36 instrument (Biorad) and was performed as described before. A summary of the kinetic and thermodynamic measurements is given in table 19. 
     Interestingly, none of the additional single mutations in the “Pertuzumab (Tras.L3) LC” variants significantly affected the affinity when combined with the Pertuzumab and the affinity was comparable to “Pertuzumab HC”×“Pertuzumab (Tras.L3) LC”. However, combination of the “Pertuzumab (Tras.L3) LC” variants with the Trastuzumab HC resulted in significant differences. While back mutations to the Pertuzumab sequence in the LCDR3 region (P94Y and T96Y) (protein sequence of variable domains listed as SEQ ID NOs: 50 and 52) completely abolished binding to Her2, introduction of Pertuzumab-specific mutations (SEQ ID NOs: 32, 34, 36, 38, 40, 42, 44, 46, and 48) yielded an equal or improved affinity compared to the initial combination “Trastuzumab HC”×“Pertuzumab (Tras.L3) LC”. In particular, introduction of the mutations I31T, G32A, Y53F, and G66R led to the most significant improvement in binding. 
     Simultaneous Introduction of Most Significant Trastuzumab-Specific Residues into the “Pertuzumab (Tras.L3) LC” 
     Based on the binding measurement described above, introduction of the 4 most relevant Trastuzumab-specific mutations into the “Pertuzumab (Tras.L3) LC” was combined. The protein sequences of the variable domains containing either the individual mutations are listed as SEQ ID NOs: 36, 40, 42, and 48.) The chain containing the “quadruple mutation” was named “Pertuzumab (Tras.L3) (QM) LC” (SEQ ID NOs: 54). 
     Affinity-Determination of the “Pertuzumab (Trast.L3)(QM) LC” by SPR 
     In order to characterize the new “Pertuzumab (Tras.L3) (QM) LC” and to determine the binding affinity when combined with either Pertuzumab HC or Trastuzumab HC, a further SPR experiment was performed. The Affinity (K D ) of the new antibody chain combinations was measured using a ProteOn XPR36 instrument (Biorad) and was performed as described before. A summary of the kinetic and thermodynamic measurements is given in  FIG. 20  and table 20. 
     Similar to the individual mutations that were introduced and characterized before, the affinity of the antibody comprising the Pertuzumab HC and the “Pertuzumab (Tras.L3) (QM) LC” did not significantly decrease compared to the initial chain combination “Pertuzumab HC”×“Pertuzumab (Tras.L3) LC”. For both chain combinations, the affinity was in the range of 26 to 34 nM. Interestingly, the combination of the “Trastuzumab HC” with the “Pertuzumab (Tras.L3) (QM) LC” leads to a very strong improvement of the affinity to Her2. Furthermore, its affinity of 200 pM is even better than the measured affinity of the parental antibody Trastuzumab (2.2 nM). 
     Given that the combination of “Pertuzumab (Tras.L3) (QM) LC” with Trastuzumab HC fully restores (or even exceeds) binding to Her2 and considering that the combination with the Pertuzumab HC diminishes but not abrogates binding to Her2, it was apparent to use the “Pertuzumab (Tras.L3) (QM) LC” as a CLC for both Her2 specificities and restore binding to the Pertuzumab-specific epitope by affinity-maturation of the Pertuzumab HC. 

 
     
       
         
           
               
             
               
                 TABLE 20 
               
             
            
               
                   
               
               
                 Kinetic and thermodynamic parameters of antibodies comprising 
               
               
                 either a Trastuzumab or Pertuzumab HC and the final CLC 
               
            
           
           
               
               
               
               
            
               
                 Clone 
                 ka [1/Ms] 
                 kd [1/s] 
                 KD [M] 
               
               
                   
               
               
                 Pertuzumab HC 
                 9.46E+04 
                 3.24E− 
                 3.42E−08 
               
               
                 Pertuzumab (Tras.L3)(QM) LC 
               
               
                 Trastuzumab HC 
                 2.69E+05 
                 5.42E−05 
                 2.02E−10 
               
               
                 Pertuzumab (Tras.L3)(QM) LC 
               
               
                   
               
            
           
         
       
     
     Example 13: Affinity Maturation of the Pertuzumab Heavy Chain 
     Generation of Pertuzumab-Based H1/H3 and H2 Affinity Maturation Libraries 
     Generation of affinity-matured Pertutzumab-derived heavy chains was carried out by phage display using standard protocols (Silacci et al, 2005). 
     For the generation of Pertuzumab-derived HCs with improved affinity when jointly expressed with “Pertuzumab (Tras.L3) (QM) LC” a maturation library randomized in CDR1 and 3 or in CDR2 was generated. The sequence of the Pertuzumab HC (SEQ ID NO: 22) and of the “Pertuzumab (Tras.L3) (QM) LC” (SEQ ID NO: 54) was cloned into a phagemid and used as a template for the randomization. For the generation of the Pertuzumab HC affinity maturation library randomized in CDR1 and 3, three fragments were assembled by “splicing by overlapping extension” (SOE) PCR and cloned into the phage vector. The following primer combinations were used to generate the library fragments: fragment 1 (LMB3 (SEQ ID NO: 147) and AM_omni_H1_TN-ba (SEQ ID NO: 148), fragment 2 (RJH108 (omni_3′H1_fo) (SEQ ID NO: 149) and RJH109 (omni_5′H3_re) (SEQ ID NO: 150), and fragment 3 (AM_omni_H3_TN_fo (SEQ ID NO: 151) and RJH99 (SEQ ID NO: 152). After assembly of sufficient amounts of full length randomized fragment, it was digested with MunI/NheI alongside with identically treated acceptor phagemid vector. 6 ug of Fab library insert were ligated with 24 ug of phagemid vector. Purified ligations were used for 60 transformations resulting in 6×10 exp 9 transformants. Phagemid particles displaying the Pertuzumab affinity maturation library were rescued and purified by PEG/NaCl purification to be used for selections. 
     The generation of the CDR2-randomized Pertuzumab HC affinity maturation library was done similarly, but only 2 fragments were generated, assembled and cloned into the phagemid using the same restriction enzymes as before. The following primer combinations were used to generate the library fragments: fragment 1 (LMB3 (SEQ ID NO: 147) and RJH110 (omni_5′H2_ba) (SEQ ID NO: 153) and fragment 2 (AM_omni_h2_TN_fo (SEQ ID NO: 154) and RJH99 (SEQ ID NO:152). Purified ligations were used for 60 transformations resulting in 4×10 exp 9 transformants. Phagemid particles displaying the Pertuzumab affinity maturation library were rescued and purified by PEG/NaCl purification to be used for selections. 
     
       
         
           
               
             
               
                 TABLE 21 a) 
               
             
            
               
                   
               
               
                 Primer combinations for the generation of the CDR1 and 
               
               
                 3-randomized Pertuzumab affinity-maturation library 
               
               
                 Pertuzumab HC affinity maturation (CDR1 and 3) 
               
            
           
           
               
               
               
            
               
                 fragment 
                 5′Primer 
                 3′Primer 
               
               
                   
               
               
                 PCR1 
                 LMB3 
                 AM_omni_H1_TN-ba 
               
               
                 PCR2 
                 RJH108 (omni_3′H1_fo) 
                 RJH109 (omni_5′H3_re) 
               
               
                 PCR3 
                 AM_omni_H3_TN_fo 
                 RJH99 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 21 b 
               
             
            
               
                   
               
               
                 Primer sequences for the generation of the CDR1 and 3-randomized Pertuzumab 
               
               
                 affinity-maturation library 
               
               
                 Pertuzumab HC affinity maturation (CDR1 and 3) 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                 Name 
                 Sequence 
               
               
                   
               
               
                 147 
                 LMB3 
                 CAGGAAACAGCTATGACCATGATTAC 
               
               
                   
               
               
                 148 
                 AM_omni_ 
                 CCGGTGCCTGACGAACCCAATCCAT4 3 2 1 
               
               
                   
                 H1_TN-ba 
                 AAAGGTAAAACCGCTTGCTGCACAGCTC 
               
               
                   
                   
                 1 T = 60%, S/G/R/N/D = 20% (4% each), rest = 20% (1.7% each) 
               
               
                   
                   
                 2 D = 60%, S/N/T/A/R/E/Q/G = 30% (3.8% each), rest = 10% (1.1% 
               
               
                   
                   
                 each) 
               
               
                   
                   
                 3 Y = 60%, F/S/H/N/D/T = 30% (5.0% each), rest = 10% (0.9%) 
               
               
                   
                   
                 4 T = 60%, A/G/V/S/P/D/N = 30% (4.3% each), rest = 10% (1.0%) 
               
               
                   
               
               
                 149 
                 RJH108 
                 ATGGATTGGGTTCGTCAGGCACCGGGTAAAGG 
               
               
                   
                 (omni_3′H1 
                   
               
               
                   
                 _fo) 
                   
               
               
                   
               
               
                 150 
                 RJH109 
                 ATTACGTGCACAATAATACACTGCGGTATCCTC 
               
               
                   
                 (omni_5′H3 
                   
               
               
                   
                 _re) 
                   
               
               
                   
               
               
                 151 
                 AM_omni_ 
                 TACCGCAGTGTATTATTGTGCACGT4a 5 5a 6 7 TTC8 
               
               
                   
                 H3_TN_fo 
                 TTTGATTATTGGGGTCAGGGCACCCTGGTTAC 
               
               
                   
                   
                 4a N = 60%, G/D/E/Q/V/S/A/P/R/L/T/Y = 40% (3.3% each) 
               
               
                   
                   
                 5 L = 60%, G/Y/S/A/D/T/R/P/V/N/W/F/I/E = 40% (2.9% each) 
               
               
                   
                   
                 5a G = 60%, Y/S/A/D/T/R/P/L/V/N/W/F/I/E = 40% (2.9% each) 
               
               
                   
                   
                 6 P = 60%, G/Y/S/A/D/T/R/L/V/N/W/F/I/E = 40% (2.9% each) 
               
               
                   
                   
                 7 S = 60%, G/Y/P/A/D/T/R/L/V/N/W/F/I/E = 40% (2.9% each) 
               
               
                   
                   
                 8 Y = 60%, G/A/P/W/S/D/T/F/R/K/H = 40% (3.6% each) 
               
               
                   
               
               
                 152 
                 RJH99 
                 GGCTGAGACTCCTCAAGAGAAGGATTAG 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 22 a) 
               
             
            
               
                   
               
               
                 Primer combinations for the generation of the 
               
               
                 CDR2-randomized Pertuzumab affinity-maturation library 
               
               
                 Pertuzumab HC affinity maturation (CDR2) 
               
            
           
           
               
               
               
               
            
               
                   
                 fragment 
                 5′Primer 
                 3′Primer 
               
               
                   
                   
               
               
                   
                 PCR1 
                 LMB3 
                 RJH110(omni_5′H2_ba) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 Table 22 b 
               
             
            
               
                   
               
               
                 Primer combinations for the generation of the CDR2-randomized Pertuzumab 
               
               
                 affinity-maturation library 
               
               
                 Pertuzumab HCaffinity maturation (CDR2) 
               
            
           
           
               
               
               
            
               
                 SEQ 
                   
                   
               
               
                 ID 
                 Name 
                 Sequence 
               
               
                   
               
               
                 153 
                 RJH110(om 
                 ATTAACATCTGCAACCCATTCCAGACCTTTAC 
               
               
                   
                 ni_5′H2_ba) 
                   
               
               
                   
               
               
                 154 
                 AM_onmi_h 
                 GGTCTGGAATGGGTTGCAGATGTTAAT9 10 11 12 GGT 
               
               
                   
                 2_TN_fo 
                 13 ATT14 AAC15 CGTTTTAAAGGTCGTTTTACCCTGAG 
               
               
                   
                   
                 9 P = 60%, G/A/S/T/D/N/F/Y = 30% (3.8% each), rest = 10% (1.0% 
               
               
                   
                   
                 each) 
               
               
                   
                   
                 10 N = 60%, S/D/G/T/R/A = 30% (5.0% each), rest = 10% (0.8%) 
               
               
                   
                   
                 11 S = 60%, G = 10%, rest = 30% (1.8% each) 
               
               
                   
               
            
           
         
       
     
     Selection of Affinity Matured Pertuzumab HC-Derived Clones 
     Selections against the extracellular domain (ECD) of human Her2 were carried out using HEK293-expressed protein. The antigen was enzymatically biotinylated by co-expression of the biotin ligase Bir A via an N-terminal avi-tag. Panning rounds were performed in solution according to the following pattern: 1. binding of ˜10 12  phagemid particles to 10 nM biotinylated Her2 ECD for 0.5 h in a total volume of 1 ml, 2. capture of biotinylated Her2 ECD and specifically bound phage particles by addition of 5.4×10 7  streptavidin-coated magnetic beads for 10 min, 3. washing of beads using 5×1 ml PBS/Tween20 and 5×1 ml PBS, 4. elution of phage particles by addition of 1 ml 100 mM TEA for 10 min and neutralization by adding 500 μl 1M Tris/HCl pH 7.4, 5. re-infection of exponentially growing  E. coli  TG1 bacteria, and 6.infection with helperphage VCSM13 and subsequent PEG/NaCl precipitation of phagemid particles to be used in subsequent selection rounds. Selections were carried out over 3 rounds using decreasing (from 20×10 −9 M to 1×10 −9 M) antigen concentrations. In round 2 and 3, capture of antigen:phage complexes was performed using neutravidin plates instead of streptavidin beads. In addition, neutravidin plates were washed for 3 h in 21 PBS. Specific binders were identified by ELISA as follows: 100 μl of 30 nM biotinylated Her2 ECD per well were coated on neutravidin plates. Fab-containing bacterial supernatants were added and binding Fabs were detected via their Flag-tags by using an anti-Flag/HRP secondary antibody. ELISA-positive clones were bacterially expressed as soluble Fab fragments in 96-well format and supernatants were subjected to a kinetic screening experiment by SPR-analysis using Proteon XPR36. 
     Affinity-Determination of Affinity-Matured Pertuzumab HC Variants by SPR 
     The Affinity (K D ) of the new Pertuzumab HC variants was measured by surface plasmon resonance. In a first step, 7000 RU of polyclonal anti-human Fab antibody were immobilized on all 6 channels of a GLM chip by Amine coupling (NaAcetate pH4.5, 30 μl/min, 300 s) (vertical orientation). 
     Each antibody-containing bacterial supernatant was filtered and 3-fold diluted with PBS, and then injected for 180 s at 30 μl/minute to achieve immobilization levels of between 100 and 400 response units (RU) in vertical orientation. Injection of Her2: For one-shot kinetics measurements, injection direction was changed to horizontal orientation, two-fold dilution series of purified Her2 (varying concentration ranges between 100 and 6.25 nM) were injected simultaneously at 100 μl/min along separate channels 1-5, with association times of 180 s, and dissociation times of 1000 s. Buffer (PBST) was injected along the sixth channel to provide an “in-line” blank for referencing. Regeneration was performed by two pulses of 10 mM glycine pH 1.5 and 50 mM NaOH for 30 s at 100 μl/min (horizontal orientation). Association rate constants (k on ) and dissociation rate constants (k off ) were calculated using a simple one-to-one Langmuir binding model in ProteOn Manager v3.1 software by simultaneously fitting the association and dissociation sensorgrams. The equilibrium dissociation constant (K D ) was calculated as the ratio k off /k on . Clones expressing Fabs with the highest affinity constants were identified and the heavy chains of the corresponding phagemids were sequenced. The thermodynamic measurement of the most affine Pertuzumab HC variants (protein sequence of variable domains listed as SEQ ID NOs: 62, 66, 68, 70, 72, and 74) is summarized in table 23 and  FIG. 21 . 
     
       
         
           
               
             
               
                 TABLE 23 
               
             
            
               
                   
               
               
                 Affinity of selected affinity matured Pertuzumab clone variants 
               
               
                 in combination with the common light chain (CLC) 
               
            
           
           
               
               
               
            
               
                   
                 Pertuzumab heavy chain 
                 affinity (nM) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 Pertuzumab 
                 34 
               
               
                   
                 Aff. mat. clone D1 
                 1 
               
               
                   
                 Aff. mat. clone B2 
                 1 
               
               
                   
                 Aff. mat. clone E1 
                 0.5 
               
               
                   
                 Aff. mat. clone G2 
                 1 
               
               
                   
                 Aff. mat. clone C8 
                 3 
               
               
                   
                 Aff. mat. clone A1 
                 1 
               
               
                   
                   
               
            
           
         
       
     
     Example 14: Characterization of the Trastuzumab/Pertuzumab Bispecific Antibodies Generation of the Trastuzumab/Pertuzumab Bi-Specific Anti-Her Antibodies with a CLC 
     In the following step, the affinity-matured Pertuzumab HC as well as the Trastuzumab HC were expressed in combination with the CLC named “Pertuzumab (Tras.L3) (QM) LC” in a bispecific antibody format. For the generation of such bispecific antibodies with a CLC, heterodimerization of the 2 different HCs was achieved by application of the knob-into-hole technology. Variable domains of the Pertuzumab affinity-matured clones E1 and G2 (protein sequence of variable domains listed as SEQ ID NOs: 68 and 70) as well as a clone “D1-derived” (D1-der) sequence (SEQ ID NOs: 64) were cloned into a human IgG1 HC containing the “hole” mutations in domain CH3. A schematic overview of the bispecific antibody with a CLC is shown in  FIG. 22 . Affinity-maturation clone “D1-der” combines the CDR1 and 3 mutations of clone D1 (SEQ ID NOs: 62) with additional CDR2 mutations found in other selected clones. The variable domain of the Tratuzumab HC (SEQ ID NO: 92) was cloned into a human IgG1 HC harboring the “knob” mutations in domain CH3. The resulting constructs, called “Herceptarg” constructs, were co-expressed and purified from mammalian-derived cell culture supernatant. A summary of the analytical data for all three bi-specific antibodies is shown clones in  FIGS. 23A-23C  and table 24. 
     
       
         
           
               
             
               
                 TABLE 24 
               
             
            
               
                   
               
               
                 Production of HER2 antibody with common light chain 
               
            
           
           
               
               
               
            
               
                   
                 yield/liter 
                   
               
               
                 Antibody 
                 (mg/l) 
                 % Monomer 
               
               
                   
               
            
           
           
               
               
               
            
               
                 Herceptarg CLC G2 
                 41.3 
                 100 
               
               
                 Herceptarg CLC E1 
                 72.1 
                 96 
               
               
                 Herceptarg CLC D1- 
                 20.1 
                 100 
               
               
                 der. 
               
               
                   
               
            
           
         
       
     
     Generation of Her2 Knock-Out Antigen Variants 
     In order to analyze and characterize binding of the Herceptarg bi-specific antibodies to each of both epitopes on Her2, Her2 knock-out variants were designed. In these variants, either of the two specific epitopes was deleted by mutation of the amino acids that interact with the respective antibody chains. 
     For expression and purification, the respective DNA fragments were fused in frame to an N-terminal leader sequence and a C-terminal human IgG1 Fc coding fragment serving as solubility- and purification tag. An avi-tag at the C-terminal end of the Fc fragment allowed in vivo biotinylation. In order to express the antigen in a monomeric form, these Fc chains contained the “knob” mutations (SEQ ID NOs: 5 and 6, Her2 ECD-Fc(knob); SEQ ID NOs: 7 and 8, Her2 ECD (Pertuzumab KO)-Fc(knob); SEQ ID NOs: 9 and 10, Her2 ECD (Trastuzumab KO)-Fc(knob)) and was co-expressed in combination with an “Fc-hole” counterpart (SEQ ID NOs: 3 and 4). 
     Affinity-Determination of the Herceptarg Bi-Specific Antibodies by SPR 
     The Affinity (K D ) of the new bi-specific antibodies to each of their epitopes in her2 was measured by SPR using a ProteOn XPR36 instrument (Biorad) at 25° C. In a first step, 11000 RU of a polyclonal goat anti-human IgG (Sigma I2136) recognizing human IgG (Fc-specific) was immobilized on all 6 channels of a GLM chip by Amine coupling (NaAcetate pH4, 30 μl/min, 300 s) (vertical orientation). 
     Each antibody was diluted with PBST (10 mM phosphate, 150 mM sodium chloride pH 7.4, 0.005% Tween 20) to 2 μg/ml, and then injected for 60 s at 30 μl/minute to achieve immobilization levels of about 400 response units (RU) in vertical orientation. Injection of Her2: For one-shot kinetics measurements, injection direction was changed to horizontal orientation, two-fold dilution series of purified monovalent Her2-Fc protein constructs (varying concentration ranges between 100 and 6.25 nM) were injected simultaneously at 100 μl/min along separate channels 1-5, with association times of 180 s, and dissociation times of 600 s. Buffer (PBST) was injected along the sixth channel to provide an “in-line” blank for referencing. Regeneration was performed by two pulses of 10 mM glycine pH 1.5 and 50 mM NaOH for 30 s at 100 μl/min (horizontal orientation). Association rate constants (k on ) and dissociation rate constants (k off ) were calculated using a simple one-to-one Langmuir binding model in ProteOn Manager v3.1 software by simultaneously fitting the association and dissociation sensorgrams. The equilibrium dissociation constant (K D ) was calculated as the ratio k off /k on . 
     All antibodies including Trastuzumab, Pertuzumab as well as three bi-specific Herceptarg constructs comprising the affinity-matured Pertuzumab HCs, the Trastuzumab HC, and the CLC “Pertuzumab (Trast.L3) (QM)” were tested for binding to both Her2 knock-out variants. As expected, both Trastuzumab and Pertuzumab bind to their respective Her2 epitope with the excepted affinity. Binding to their corresponding knock-out variant was abolished ( FIG. 24 ). These results demonstrate that the use of Her2 knock-out epitope variants allows dissecting the binding of the bispecific antibodies and analyzing the individual affinity of both specificities. 
     Determination of the individual K D  values of each Herceptarg clone variant revealed a constant binding affinity to the Trastuzumab epitope in the expected range of 0.6 to 1.8 nM. In contrast, binding to the Pertuzumab epitope depends on the affinity-matured Pertuzumab HC. Among the three Herceptarg clone variants, clone “D1-der” was found to have the highest affinity (0.16 nM). A summary of the Thermodynamic data is shown in table 25. In summary, this experiment confirms that we were able to generate a bi-specific antibody with a CLC and specific for the epitopes Trastuzumab and Pertuzumab. 
     Binding Analysis of the Herceptarg Clone Variants to KPL-4 Cells 
     KPL-4 cells were harvested and resuspended in FACS buffer. 0.2 Mio cells were seeded into a 96 well round bottom plate. The plate was centrifuged at 400 g for 3 min to pellet the cells. The supernatant was removed and the cells were resuspended in 40 μl of the diluted antibodies. The plate was incubated for 30 min at 4° C. to allow binding of the antibodies. To remove unbound antibodies the cells were centrifuged again and washed twice with FACS buffer. To detect the antibodies the cells were resuspended in 12 μl diluted secondary goat anti-human Fc specific FITC-labeled secondary antibody (Jackson ImmunoResearch #109-096-098) and incubated again for 30 min at 4° C. Afterwards the cells were washed twice with FACS buffer, resuspended in 200 μl FACS buffer and the fluorescence was measured with BD CantoII. Results are shown in  FIG. 25 . 
     
       
         
           
               
             
               
                 TABLE 25 
               
             
            
               
                   
               
               
                 Affinity of selected bi-specific antibodies affinity matured 
               
               
                 Pertuzumab clone variants in combination with the CLC 
               
            
           
           
               
               
               
            
               
                   
                 KD (nM) Trastuzumab 
                 KD (nM) Pertuzumab 
               
               
                 clone 
                 Epitope 
                 Epitope 
               
               
                   
               
            
           
           
               
               
               
            
               
                 Trastuzumab 
                 2.17 
                 N/A 
               
               
                 Pertuzumab 
                 N/A 
                 0.62 
               
               
                 Herceptarg E1 
                 0.72 
                 1.32 
               
               
                 Herceptarg G2 
                 1.82 
                 9.65 
               
               
                 Herceptarg D1-derived 
                 0.6 
                 0.16 
               
               
                 Herceptarg crossmab 
                 0.8 
                 0.84 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 26 
               
               
                   
               
               
                 Proliferation inhibition of various cell types (IC50 values with confidence interval) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 cell 
                   
                   
                 Trastuzumab + 
                 Herceptarg 
               
               
                 line 
                 Trastuzumab 
                 Pertuzumab 
                 Pertuzumab 
                 CLC E1/1 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 BT474 
                 110.8 
                 (96.7-127.0) 
                 205.4 
                 (156-270.7) 
                 222.3 
                 (176.5-280.1) 
                 114.1 
                 (106-122.7) 
               
               
                 N87 
                 83.13 
                 (59.7-115.7) 
                 205.8 
                 (126.3-335) 
                 312.9 
                 (156.5-625.6) 
                 136.5 
                 (126.6-147) 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 SkBr3 
                 73.73 
                 (43.54-124.8) 
                 nd 
                 55.63 
                 (25.97-119.2) 
                 69.01 
                 (55.78-85.4) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 cell 
                 Herceptarg 
                 Herceptarg 
                   
               
               
                   
                 line 
                 CLC D1-der 
                 CLC G2/2 
                 GA604 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 BT474 
                 78.38 
                 (69.03-89) 
                 89.99 
                 (82.3-98.4) 
                 125.4 
                 (113.6-138.4) 
               
               
                   
                 N87 
                 92.7 
                 (80.7-106.5) 
                 119.1 
                 (107.6-132) 
                 109.6 
                 (98.3-122.2) 
               
               
                   
                 SkBr3 
                 47.54 
                 (26.61-84.9) 
                 92.59 
                 (70.1-122.3) 
                 59.84 
                 (50.2-71.4) 
               
               
                   
                   
               
            
           
         
       
     
     Proliferation Inhibition Mediated by the Herceptarg Binding Variants 
     Target cells were harvested, washed, resuspended in RPMI 1640 (Gibco)+10% FCS+1% GlutaMAX™ (Gibco) and plated at a concentration of 5×10 3  cells/well. Cells were incubated for 4 hours in the cell incubator before respective antibody dilutions were added. Plates were gently shaked and incubated for 5 days in the cell incubator. The plates were equilibrated to room temperature and 100 μl/well of the freshly prepared CellTiter Glo (Promega) substrate were added to each well. Luminescence was measured in a Wallac Victor3 1420 Multilabel Counter. Results are shown in Table 26 and  FIGS. 26A-26C . 
     Herceptarg Clones-Mediated Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) 
     Target cells were harvested, washed, resuspended in AIM V® medium (Life Technologies), and plated at a concentration of 3×10 4  cells/well. The respective antibody dilutions were added in triplicates to the cells and incubated for 10 min before addition of the effector cells (peripheral blood mononuclear effector cells [PBMCs]). Effector (E) and target (T) cells were then incubated for the indicated time at 37° C. at the indicated E:T ratio (triplicates for all samples). Lactate dehydrogenase (LDH) release was measured using the LDH Cytotoxicity Detection Kit (Roche Applied Science). ADCC was calculated using the following formula: 
     
       
         
           
             
               Percentage 
                
               
                   
               
                
               ADCC 
             
             = 
             
               
                 ( 
                 
                   [ 
                   
                     
                       
                         sample 
                          
                         
                             
                         
                          
                         release 
                       
                       - 
                       
                         spontaneous 
                          
                         
                             
                         
                          
                         release 
                       
                     
                     
                       
                         maximal 
                          
                         
                             
                         
                          
                         release 
                       
                       - 
                       
                         spontaneous 
                          
                         
                             
                         
                          
                         release 
                       
                     
                   
                   ] 
                 
                 ) 
               
               × 
               100. 
             
           
         
       
     
     Spontaneous release, corresponding to target cells incubated with effector cells without antibody, was defined as 0% cytotoxicity, and maximal release (target cells lysed with 1% Triton X-100) was defined as 100% cytotoxicity. The average percentage of ADCC and standard deviations of the triplicates of each experiment were calculated. Results are shown in  FIGS. 27A and 27B . 
     
       
         
           
               
             
               
                 TABLE 27 
               
             
            
               
                   
               
               
                 Leader Peptides 
               
            
           
           
               
               
            
               
                 SEQ ID 
                 Protein sequence 
               
               
                   
               
               
                 155 
                 MDWTWRILFLVAAATGAHS 
               
               
                   
               
               
                 156 
                 MDMRVPAQLLGLLLLWFPGAR 
               
               
                   
                 C 
               
               
                   
               
               
                 157 
                 MGWSCIILFLVATATGVHS 
               
               
                   
               
            
           
         
       
     
     Example 15: Proliferation Assay 
     1×10 4  BT-474 cells/well were cultured in RPMI/10% FCS in a 96-well flat bottom plate. After 24 hrs growth medium was removed and titrated amounts of indicated antibodies were added (premixed in culture medium) to a final volume of 100 μl. 
     To determine the number of viable cells in culture, the CellTiter-Glo Luminescent Cell Viability Assay was performed by quantifying the present ATP levels as an indicator of metabolically active cells. Thus, after six days of culture, 100 μl CellTiter-Glo Reaction Mix (Promega, cat. no. # G7571) was added to the cells, shook for 2 min before 75 μl of the lysate was transferred to a separate 96-well flat bottom titer plate (Costar, cat. no. #3917). After additional mixing, luminescence was assed according to the manufacturer&#39;s instructions using a Tecan Infinite Reader. 
     Results are shown in table 28 and  FIG. 28 . 
     In the proliferation assay it was shown that the bispecific Her2 antibodies inhibited proliferation of BT-474 cells more potently than Pertuzumab or Trastuzumab alone or in combination. The following bispecific Her2 antibodies were tested: Herceptarg CLC D1-der wt”: SEQ ID NOs 64, 54, 92, Herceptarg CLC D1-der G2”: SEQ ID NOs 64, 54, 92 (glycoengineered variant) “Herceptarg CrossMab”: SEQ ID NOs 109, 110, 111, 112. 
     
       
         
           
               
             
               
                 TABLE 28 
               
             
            
               
                   
               
               
                 IC50 BT474 proliferation assay 
               
            
           
           
               
               
               
            
               
                   
                 Antibody treatment 
                 IC50 Proliferation [nM] 
               
               
                   
                   
               
               
                   
                 Trastuzumab 
                 n.d. 
               
               
                   
                 Pertuzumab 
                 n.d. 
               
               
                   
                 Trastuzumab + Pertuzumab 
                 6.20 
               
               
                   
                 Herceptarg CLC D1-der. wt 
                 3.31 
               
               
                   
                 Herceptarg CLC D1-der. G2 
                 3.93 
               
               
                   
                 Herceptarg CrossMab 
                 4.75 
               
               
                   
                   
               
            
           
         
       
     
     Example 16: C1q FACS Binding Assay 
     3×10 5  BT-474 cells were incubated with 10 μg/ml of indicated antibody on ice. The following bispecific Her2 antibodies were tested: Herceptarg CLC D1-der wt”: SEQ ID NOs 64, 54, 92, Herceptarg CLC D1-der G2”: SEQ ID NOs 64, 54, 92 (glycoengineered variant) “Herceptarg CrossMab”: SEQ ID NOs 109, 110, 111, 112. 
     After 30 min, 10 μg/ml C1q (Sigma, C1740) was added and additionally incubated for 20 min. After washing, cells were counterstained with commercial PE-labeled anti-C1q antibody (Cedarlane, CL7611PE-SP). After further incubation (30 min, ice), cells were washed twice and analyzed on a FACS Canto II. 
     Results are shown in  FIG. 29  and table 29. This C1q assay illustrates the binding of recombinant complement factor C1q to different Her antibodies on BT-474 cells. It was shown that the highest C1q binding resulted upon treatment with the combination of Trastuzumab and Pertuzumab, followed by the two CLC bispecific Her2 antibodies. Treatment with the Crossmab resulted only in a slightly elevated C1q binding. 
     
       
         
           
               
             
               
                 TABLE 29 
               
             
            
               
                   
               
               
                 C1q binding assay 
               
            
           
           
               
               
               
            
               
                   
                   
                 PE-signal 
               
               
                   
                 antibody/antibodies 
                 (geomean) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 trastuzumab 
                 282 
               
               
                   
                 pertuzumab 
                 344 
               
               
                   
                 combination of trastuzumab and pertuzumab 
                 2157 
               
               
                   
                 bispecific anti-HER2 antibody, common light chain 
                 1439 
               
               
                   
                 bispecific anti-HER2 antibody, common light chain, 
                 1036 
               
               
                   
                 glycoengineered 
               
               
                   
                 bispecific anti-HER2 antibody, CrossMab format 
                 489 
               
               
                   
                   
               
            
           
         
       
     
     Example 17: LDH Assay with Baby Rabbit Complement (BRC) 
     CHO-K1 Nxre19 cells (IL15R transfected CHO-K1) were seeded at 10,000 cells/well on 96-well flat bottom cell culture plates (NUNC, 100 μL/well) and cultivated overnight. IL15-Fc fusion polypeptide was added (25 μL/well in 5-fold end-concentration) and incubated for one hour. Thereafter, one vial of Baby Rabbit complement (Cedarlane, Cat. No. CL3441) was reconstituted with 1 mL of Aqua bidest. The complement solution was diluted with medium and 25 μL added to the wells. After four hours the plates were centrifuged at 200 g and 100 μL/well were transferred to another 96-well flat bottom plate. Thereafter 100 μL of LDH reaction mix (Cytotoxicity Detection Kit, Roche Diagnostic GmbH, Mannheim, Germany) was added. After an incubation time of 20 min. at 37° C. optical density (OD) was measured at 492/690 nm on a Tecan Sunrise reader. 
                     TABLE 30                  LDH assay with BRC                             signal [OD]                             sample   BRC 1/40   BRC 1/30                                 9000 ng/ml IL15-Fc-fusion with HUC   11.3   12.3       3000 ng/ml IL15-Fc-fusion with HUC   12.3   17.0       1000 ng/ml IL15-Fc-fusion with HUC   10.2   13.6       333.3 ng/ml IL15-Fc-fusion with HUC   7.8   12.2       111.1 ng/ml IL15-Fc-fusion with HUC   8.3   13.0       37.04 ng/ml IL15-Fc-fusion with HUC   14.9   19.7       12.35 ng/ml IL15-Fc-fusion with HUC   43.2   53.0       4.12 ng/ml IL15-Fc-fusion with HUC   41.5   63.8       0 ng/ml IL15-Fc-fusion with HUC   42.4   48.4                    
It can be seen that BRC has a low background toxicity and shows dose dependent complement toxicity.
 
     Example 18: CDC (Complement Dependent Cytotoxicity) Activation on BT-474 Cells (LDH Release) 
     1×10 4  cells/well were incubated with 10 μg/ml of the indicated antibodies for 30 min at 37° C. in 150 μl. The following bispecific Her2 antibodies were tested: Herceptarg CLC D1-der wt”: SEQ ID NOs 64, 54, 92, Herceptarg CLC D1-der G2”: SEQ ID NOs 64, 54, 92 (glycoengineered variant) “Herceptarg CrossMab”: SEQ ID NOs 109, 110, 111, 112. 
     Then 50 μl Baby Rabbit Complement (Cedarlane, cat. no. CL3441, batch no. 6312) was added and incubated for further 2 hrs. Then, the s/n was transferred and mixed with 50 μl LDH Reaction Mix (Roche) and, after a further incubation of 15 min, extinktion (Ex.) at 490/620 nm was analyzed on a Tecan Sunrise Reader. The specific antibody dependent toxicity (mean+/−SD of n=4) on BT-474 cell was calculated as follows: 
       (Ex. sample−Ex. spontanous lysis/Ex. maximal lysis−spontanous lysis)×100.
 
     Results are shown in  FIG. 30 . 
     This CDC assay shows the release of LDH as a marker for dying/dead cells upon treatment of different anti-Her2 antibodies (formats, combination) in the presence of baby rabbit complement. 
     Here, the combination of Trastuzumab and Pertuzumab resulted in a significant induction of CDC, whereas the parental antibodies alone did not. Surprisingly, both CLC Herceptarg variants provoked even superior CDC effects, whereas the Herceptarg Crossmab treatment results in a CDC reaction less effective than the combination of the parental antibodies. 
     Example 19: CDC (Complement Dependent Cytotoxicity)-Mediated Killing of BT-474 Cells (ACEA) 
     1×10 4  BT-474 cells/well were seeded on 96-well E-Plates (ACEA Biosciences Inc.) and grown overnight in an Xcelligence device. Growth medium was removed and cells were washed once with serum-free AIM-V medium (Gibco). 50 μl/well AIM-V medium and 50 μl antibody in AIM-V (3-fold end concentration) were added and incubated for 20 min. 50 μl Baby Rabbit Complement (Cedarlane) was added and CellIndex (CI; as representative for the viability of the cells) was measured every 5 minutes (see curve). The following bispecific Her2 antibodies were tested: Herceptarg CLC D1-der wt”: SEQ ID NOs 64, 54, 92, Herceptarg CLC D1-der G2”: SEQ ID NOs 64, 54, 92 (glycoengineered variant) “Herceptarg CrossMab”: SEQ ID NOs 109, 110, 111, 112. 
     Specific CDC was calculated according following formula, whereas CI is the normalized cell index: 
     
       
         
           
             
               % 
                
               
                   
               
                
               CDC 
             
             = 
             
               
                 
                   
                     CI 
                      
                     
                         
                     
                      
                     Complement 
                      
                     
                         
                     
                      
                     control 
                   
                   - 
                   
                     CI 
                      
                     
                         
                     
                      
                     sample 
                   
                 
                 
                   CI 
                    
                   
                       
                   
                    
                   Complement 
                    
                   
                       
                   
                    
                   control 
                 
               
               × 
               100 
             
           
         
       
     
     At two representative time points (1 hr and 2 hrs after starting the reaction, specific lysis (=CDC-induced cell death) was calculated and shown in the diagram (mean+/SEM of n=4). 
     Results are shown in  FIGS. 31A and 31B  and table 31. This CDC assay illustrates a change in the cell index as a marker for dying/dead cells upon treatment with different anti-Her2 antibodies (formats, combination) in the presence of baby rabbit complement: Here, the combination of Trastuzumab and Pertuzumab resulted in a significant induction of CDC, whereas the parental antibodies alone did not. Surprisingly, both CLC Herceptarg variants provoked even superior CDC effects, whereas the Herceptarg Crossmab treatment results in a CDC reaction less effective than the combination of the parental antibodies. 
     One possible reason for the superiority of Herceptarg CLC D1-der may be the slightly higher affinity to the Trastuzumab epitope as well as the significantly higher affinity to the Pertuzumab epitope (see also table 25). 
     
       
         
           
               
             
               
                 TABLE 31 
               
             
            
               
                   
               
               
                 CDC (complement dependent cytotoxicity)- 
               
               
                 mediated killing of BT-474 cells (ACEA) 
               
            
           
           
               
               
            
               
                   
                 specific lysis [% cell index 
               
               
                   
                 ACEA] 
               
            
           
           
               
               
               
            
               
                 antibody/antibodies 
                 1 hour 
                 2 hours 
               
               
                   
               
               
                 trastuzumab 
                 −3.5 ± 0.6 
                 −6.5 ± 0.8 
               
               
                 pertuzumab 
                 −5.3 ± 1.0 
                 −8.3 ± 2.1 
               
               
                 combination of trastuzumab and 
                 20.9 ± 6.7 
                 26.3 ± 7.0 
               
               
                 pertuzumab 
               
               
                 bispecific anti-HER2 antibody, common 
                 31.8 ± 3.4 
                 38.9 ± 3.7 
               
               
                 light chain (D1 der) 
               
               
                 bispecific anti-HER2 antibody, common 
                 28.8 ± 2.6 
                 35.8 ± 2.6 
               
               
                 light chain, glycoengineered (D1 der) 
               
               
                 bispecific anti-HER2 antibody, 
                 12.9 ± 1.4 
                 22.7 ± 1.6 
               
               
                 CrossMab format 
               
               
                   
               
            
           
         
       
     
     Example 20: Mouse Xenograft Studies 
     Cell Line KPL4 
     This human breast cancer cell line has been established from the malignant pleural effusion of a breast cancer patient with an inflammatory skin metastasis. Cells have been provided by Professor J. Kurebayashi (Kawasaki Medical School, Kurashiki, Japan). Tumor cells were routinely cultured in DMEM medium (PAN Biotech, Germany) supplemented with 10% fetal bovine serum (PAN Biotech, Germany) and 2 mM L-glutamine (PAN Biotech, Germany) at 37° C. in a water-saturated atmosphere at 5% CO2. Culture passage was performed with trypsin/EDTA 1× (PAN) splitting twice/week. Cell passage P6 was used for in vivo study. 
     Mice 
     Female SCID beige (C.B.-17) mice; age 10-12 weeks; body weight 18-20 g (Charles River Germany, Sulzfeld); body weight &gt;20 g are maintained under specific-pathogen-free condition with daily cycles of 12 h light/12 h darkness according to international guidelines (GV-Solas; Felasa; TierschG). After arrival animals were housed in the quarantine part of the animal facility for one week to get accustomed to new environment and for observation. Continuous health monitoring was carried out on regular basis. Diet food (Alltromin) and water were provided ad libitum. The experimental study was reviewed and approved by local government. 
     Tumor Cell Injection 
     At the day of injection tumor cells were harvested (trypsin-EDTA) from culture flasks (Greiner TriFlask) and transferred into 50 ml culture medium, washed once and resuspended in PBS. After an additional washing step with PBS and filtration (cell strainer; Falcon Ø 100 μm) the final cell titer was adjusted to 1.5×10e8/ml. Tumor cell suspension was carefully mixed with transfer pipette to avoid cell aggregation. Anesthesia was performed using a Stephens inhalation unit for small animals with preincubation chamber (plexiglas), individual mouse nose-mask (silicon) and not flammable or explosive anesthesia compound Isoflurane (Pharmacia-Upjohn, Germany) in a closed circulation system. Two days before injection, coat of the SCID beige mice were shaved and KPL-4 cells (3×10e6 cells) were injected orthotopically in a volume of 20 μl (using a Hamilton microliter syringe and a 30 G×½″ needle) into the right penultimate inguinal mammary fat pad of each anesthetized mouse. The cell suspension was injected through the skin under the nipple. 
     Monitoring 
     Animals were controlled daily for detection of clinical symptoms of adverse effects. For monitoring throughout the experiment the body weight of the animals was documented two times weekly and the tumor volume was measured by caliper twice weekly. Tumor volume was calculated according to NCI protocol (Tumor weight=½ab2, where “a” and “b” are the long and the short diameters of the tumor, respectively). Termination criteria were the critical tumor mass (up to 1.7 g or Ø&gt;1.5 cm), body weight loss more than 20% from baseline, tumor ulceration or poor general condition of the animals. Study exclusion criteria for the animals are described and approved in the corresponding “Tierversuchsanzeige”. 
     Treatment 
     Mice were randomized for tumor volume of 80 mm 3  and subsequently treated once weekly with a volume of 10 ml/kg intra peritoneal. For combination treatment Herceptin was given first and Perjeta was given 24 hrs thereafter. 
     The following bispecific Her2 antibodies was tested: Herceptarg CLC-D1-der: SEQ ID NOs 64, 54, 92. Results are shown in  FIG. 32 . 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                   
                   
                   
                   
                 Route/Mode of 
               
               
                 Group 
                 No of animals 
                 Compound 
                 Dose (mg/kg) 
                 administration 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 1 
                 9 
                 Control (Xolair) 
                 10 
                 i.p. once weekly 
               
               
                 2 
                 9 
                 Herceptin 
                 10 
                 i.p. once weekly 
               
               
                 3 
                 9 
                 Perjeta 
                 10 
                 i.p. once weekly 
               
               
                 4 
                 9 
                 Herceptin plus Perjeta 
                 10 plus 10 
                 i.p. once weekly 
               
               
                 5 
                 8 
                 Herceptarg CLC-D1- 
                 10 
                 i.p. once weekly 
               
               
                   
                   
                 der 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 32 
               
             
            
               
                   
               
               
                 Parent sequences of Pertuzumab and Trastuzumab 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO 
                 Name 
                 Sequence 
               
               
                   
               
            
           
           
               
            
               
                 Pertuzumab wt (parent) sequences 
               
            
           
           
               
               
               
            
               
                 22 
                 Pertuzumab wt 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFTDYTMDWVRQAPG 
               
               
                   
                 VH (10289) 
                 KGLEWVADVNPNSGGSIYNQRFKGRFTLSVDRSKNTLYLQMN 
               
               
                   
                   
                 SLRAEDTAVYYCARNLGPSFYFDYWGQGTLVTVSS 
               
               
                   
               
               
                 21 
                 Pertuzumab wt 
                 GAGGTGCAGCTGGTCGAGTCTGGCGGCGGACTGGTGCAGCC 
               
               
                   
                 VH 
                 TGGCGGCAGCCTGAGACTGAGCTGCGCCGCCAGCGGCTTCA 
               
               
                   
                 (10289)DNA 
                 CCTTCACCGACTACACCATGGACTGGGTGCGGCAGGCCCCT 
               
               
                   
                   
                 GGCAAGGGCCTGGAATGGGTGGCCGACGTGAACCCCAACAG 
               
               
                   
                   
                 CGGCGGCAGCATCTACAACCAGCGGTTCAAGGGCCGGTTCA 
               
               
                   
                   
                 CCCTGAGCGTGGACAGAAGCAAGAACACCCTGTACCTCCAG 
               
               
                   
                   
                 ATGAACAGCCTGCGGGCCGAGGACACCGCCGTGTACTACTG 
               
               
                   
                   
                 CGCCCGGAACCTGGGCCCCAGCTTCTACTTCGACTACTGGGG 
               
               
                   
                   
                 CCAGGGCACCCTGGTGACCGTGAGCAGCGCTAGCACCAAGG 
               
               
                   
                   
                 GCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCT 
               
               
                   
                   
                 CTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTAC 
               
               
                   
                   
                 TTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCT 
               
               
                   
                   
                 GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTC 
               
               
                   
                   
                 AGGACTCTACTCCCTCAGCAGC 
               
               
                   
               
               
                 14 
                 Pertuzumab wt 
                 
                   GFTFTDYTMD 
                 
               
               
                   
                 VH CDR1 
                   
               
               
                   
               
               
                 15 
                 Pertuzumab wt 
                 
                   DVNPNSGGSIYNQRFKG 
                 
               
               
                   
                 VH CDR2 
                   
               
               
                   
               
               
                 16 
                 Pertuzumab wt 
                 
                   NLGPSFYFDY 
                 
               
               
                   
                 VH CDR3 
                   
               
               
                   
               
               
                 114 
                 Pertuzumab wt 
                 
                   ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWN 
                 
               
               
                   
                 CH1 
                 
                   SGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNV 
                 
               
               
                   
                   
                 
                   NHKPSNTKVDKKV 
                 
               
               
                   
               
               
                 24 
                 Pertuzumab wt 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 VL 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10290) 
                 QQYYIYPYTFGQGTKVEIK 
               
               
                   
               
               
                 23 
                 Pertuzumab wt 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 VL 
                 CGTGGGCGACAGAGTGACCATCACCTGCAAGGCCAGCCAGG 
               
               
                   
                 (10290)DNA 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACAGGCGTGCCCAGCCGGTTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGTACTACATCTACCCCTAC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAGATCAAG 
               
               
                   
               
               
                 11 
                 Pertuzumab wt 
                 
                   KASQDVSIGVA 
                 
               
               
                   
                 VL CDR1 
                   
               
               
                   
               
               
                 12 
                 Pertuzumab wt 
                 
                   SASYRYT 
                 
               
               
                   
                 VL CDR2 
                   
               
               
                   
               
               
                 13 
                 Pertuzumab wt 
                 
                   QQYYIYPYT 
                 
               
               
                   
                 VL CDR3 
                   
               
               
                   
               
               
                 113 
                 Pertuzumab wt 
                 
                   RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQW 
                 
               
               
                   
                 CL 
                 
                   KVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKH 
                 
               
               
                   
                   
                 
                   KVYACEVTHQGLSSPVTKSFNRGEC 
                 
               
               
                   
               
            
           
           
               
            
               
                 Trastuzumab wt (parent) sequences 
               
            
           
           
               
               
               
            
               
                 82 
                 Trastuzumab 
                 DIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKA 
               
               
                   
                 VL (4245) 
                 PKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQ 
               
               
                   
                   
                 QHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 81 
                 Trastuzumab 
                 GACATCCAGATGACCCAGAGCCCAAGCTCTCTGTCTGCCTCT 
               
               
                   
                 VL(4245)DNA 
                 GTGGGCGACAGAGTGACCATCACCTGCAGAGCCAGCCAGGA 
               
               
                   
                   
                 CGTGAACACAGCCGTGGCCTGGTATCAGCAGAAGCCAGGCA 
               
               
                   
                   
                 AGGCCCCAAAGCTGCTGATCTACAGCGCCAGCTTCCTGTACA 
               
               
                   
                   
                 GCGGCGTGCCAAGCAGATTCAGCGGCAGCAGAAGCGGCACA 
               
               
                   
                   
                 GACTTCACCCTGACCATCAGCAGCCTGCAGCCAGAGGACTT 
               
               
                   
                   
                 CGCCACCTACTACTGCCAGCAGCACTACACCACCCCACCAA 
               
               
                   
                   
                 CCTTCGGACAGGGCACCAAGGTGGAGATCAAG 
               
               
                   
               
               
                 17 
                 Trastuzumab wt 
                 
                   RASQDVNTAVA 
                 
               
               
                   
                 VL CDR1 
                   
               
               
                   
               
               
                 18 
                 Trastuzumab wt 
                 
                   SASFLYS 
                 
               
               
                   
                 VL CDR2 
                   
               
               
                   
               
               
                 19 
                 Trastuzumab wt 
                 
                   QQHYTTPPT 
                 
               
               
                   
                 VL CDR3 
                   
               
               
                   
               
               
                 116 
                 Trastuzumab wt 
                 
                   RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQW 
                 
               
               
                   
                 CL 
                 
                   KVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKH 
                 
               
               
                   
                   
                 
                   KVYACEVTHQGLSSPVTKSFNRGEC 
                 
               
               
                   
               
               
                 92 
                 Trastuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGK 
               
               
                   
                 VH 
                 GLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSL 
               
               
                   
                 (1345) 
                 RAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSS 
               
               
                   
               
               
                 91 
                 Trastuzumab 
                 GAAGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACC 
               
               
                   
                 VH 
                 GGGCGGCAGCCTGCGTCTGAGCTGCGCGGCCTCCGGATTTA 
               
               
                   
                 (11345)DNA 
                 ACATAAAGGACACATACATCCACTGGGTGCGCCAAGCACCT 
               
               
                   
                   
                 GGGAAGGGTCTCGAGTGGGTGGCTCGGATTTACCCAACAAA 
               
               
                   
                   
                 TGGCTACACCAGGTATGCGGATAGCGTGAAAGGCCGTTTTA 
               
               
                   
                   
                 CCATTTCAGCTGATACTTCGAAGAACACCGCCTATCTGCAAA 
               
               
                   
                   
                 TGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCT 
               
               
                   
                   
                 CGCGTTGGGGAGGAGACGGGTTCTATGCTATGGATTACTGG 
               
               
                   
                   
                 GGCCAAGGCACCCTGGTGACGGTTAGCTCA 
               
               
                   
               
               
                 20 
                 Trastuzumab wt 
                 
                   GFNIKDTYIH 
                 
               
               
                   
                 VH CDR1 
                   
               
               
                   
               
               
                 29 
                 Trastuzumab wt 
                 
                   RIYPTNGYTRYADSVKG 
                 
               
               
                   
                 VH CDR2 
                   
               
               
                   
               
               
                 30 
                 Trastuzumab wt 
                 
                   WGGDGFYAMDY 
                 
               
               
                   
                 VH CDR3 
                   
               
               
                   
               
               
                 115 
                 Trastuzumab wt 
                 
                   ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWN 
                 
               
               
                   
                 CH1 
                 
                   SGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNV 
                 
               
               
                   
                   
                 
                   NHKPSNTKVDKKV 
                 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 33 
               
             
            
               
                   
               
               
                 Sequences of antibodies with common light chain 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO 
                 Name 
                 Sequence 
               
               
                   
               
            
           
           
               
            
               
                 Pertuzumab / Trastuzumab hybrid light chains 
               
            
           
           
               
               
               
            
               
                 26 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Trast. L3) 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10403) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 25 
                 Pertuzumab VL 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Trast. L3) 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (10403)DNA 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 28 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Trast. H91) 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10404) 
                 QQ   H   YIYPYTFGQGTKVEIK 
               
               
                   
               
               
                 27 
                 Pertuzumab VL 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Trast. H91) 
                 CGTGGGCGACAGAGTGACCATCACCTGCAAGGCCAGCCAGG 
               
               
                   
                 (10404)DNA 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACAGGCGTGCCCAGCCGGTTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACATCTACCCCTAC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAGATCAAG 
               
               
                   
               
               
                 32 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITC   R   ASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) K24R 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10949) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 31 
                 Pertuzumab VL 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Tras.L3) K24R 
                 CGTGGGCGACAGAGTGACCATCACATGCCGGGCCAGCCAGG 
               
               
                   
                 (10949)DNA 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 34 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITCKASQDV   N   IGVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) S30N 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10950) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 33 
                 Pertuzumab 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 VL(Tras.L3) 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 S30N 
                 ACGTGAACATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                 (10950)DNA 
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 36 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVS   T   GVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) I31T 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10951) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 35 
                 Pertuzumab VL 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Tras.L3) I31T 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (10951)DNA 
                 ACGTGTCCACCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 38 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVS   V   GVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) I31V 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10952) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 37 
                 Pertuzumab VL 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Tras.L3) I31V 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (10952)DNA 
                 ACGTGTCCGTCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 40 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSI   A   VAWYQQKPGKA 
               
               
                   
                 (Tras.L3) G32A 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10953) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 39 
                 Pertuzumab VL 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Tras.L3) G32A 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (10953)DNA 
                 ACGTGTCCATCGCCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 42 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) Y53F 
                 PKLLIYSAS   F   RYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10954) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 41 
                 Pertuzumab 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 VL(Tras.L3) 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 Y53F 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                 (10954)DNA 
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTTCCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 44 
                 Pertuzumab VL 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) R54L 
                 PKLLIYSASY   L   YTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10955) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 43 
                 Pertuzumab 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Tras.L3) R54L 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (10955)DNA 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCTGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 46 
                 Pertuzumab 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) T56S 
                 PKLLIYSASYRY   S   GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10956) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 45 
                 Pertuzumab 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Tras.L3) T56S 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (10956)DNA 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CAGCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 48 
                 Pertuzumab 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) G66R 
                 PKLLIYSASYRYTGVPSRFSGS   R   SGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10957) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 47 
                 Pertuzumab 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Tras.L3) G66R 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (10957)DNA 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCCGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 50 
                 Pertuzumab 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) T94Y 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10958) 
                 QQHYT Y PPTFGQGTKVEIK 
               
               
                   
               
               
                 49 
                 Pertuzumab 
                 ACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAGC 
               
               
                   
                 (Tras.L3) T94Y 
                 GTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGGA 
               
               
                   
                 (10958)DNA 
                 CGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGCA 
               
               
                   
                   
                 AGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTAC 
               
               
                   
                   
                 ACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCAC 
               
               
                   
                   
                 CGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGACTT 
               
               
                   
                   
                 CGCCACCTACTACTGCCAGCAGCACTACACCTACCCCCCCAC 
               
               
                   
                   
                 CTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 52 
                 Pertuzumab 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKA 
               
               
                   
                 (Tras.L3) P96Y 
                 PKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (10959) 
                 QQHYTTP   Y   TFGQGTKVEIK 
               
               
                   
               
               
                 51 
                 Pertuzumab 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAG 
               
               
                   
                 (Tras.L3) P96Y 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (10959)DNA 
                 ACGTGTCCATCGGCGTGGCCTGGTATCAGCAGAAGCCCGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTACCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCGGCTCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCAGCCTGCAGCCCGAGGAC 
               
               
                   
                   
                 TTCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCTAC 
               
               
                   
                   
                 ACCTTCGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 54 
                 Pertuzumab 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSTAVAWYQQKPGKA 
               
               
                   
                 (Tras.L3)(QM) 
                 PKLLIYSASFRYTGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQ 
               
               
                   
                 (11055) 
                 QHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 53 
                 Pertuzumab 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGTCTGCCAG 
               
               
                   
                 (Tras.L3)(QM) 
                 CGTGGGCGACAGAGTGACCATCACATGCAAGGCCAGCCAGG 
               
               
                   
                 (11055)DNA 
                 ACGTGTCCACAGCCGTGGCCTGGTATCAGCAGAAGCCTGGC 
               
               
                   
                   
                 AAGGCCCCCAAGCTGCTGATCTACAGCGCCAGCTTCCGGTA 
               
               
                   
                   
                 CACCGGCGTGCCCAGCAGATTCAGCGGCAGCAGATCCGGCA 
               
               
                   
                   
                 CCGACTTCACCCTGACCATCAGCTCCCTGCAGCCCGAGGACT 
               
               
                   
                   
                 TCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCCA 
               
               
                   
                   
                 CATTTGGCCAGGGCACCAAGGTGGAAATCAAG 
               
               
                   
               
               
                 89 
                 Pertuzumab 
                 
                   KASQDVSTAVA 
                 
               
               
                   
                 (Tras.L3)(QM)- 
                   
               
               
                   
                 CDR1 
                   
               
               
                   
               
               
                 90 
                 Pertuzumab 
                 
                   SASFRYT 
                 
               
               
                   
                 (Tras.L3)(QM)- 
                   
               
               
                   
                 CDR2 
                   
               
               
                   
               
               
                 19 
                 Pertuzumab 
                 
                   QQHYTTPPT 
                 
               
               
                   
                 (Tras.L3)(QM)- 
                   
               
               
                   
                 CDR3 
                   
               
               
                   
               
            
           
           
               
            
               
                 Pertuzumab / Trastuzumab hybrid light chain affinity matured VH clones 
               
            
           
           
               
               
               
            
               
                 62 
                 Pertuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFNDYTMDWVRQAPG 
               
               
                   
                 aff.mat. clone 
                 KGLEWVADVNPNSGGSIYNQRFKGRFTLSVDRSKNTLYLQMN 
               
               
                   
                 D1 
                 SLRAEDTAVYYCARNLGPFFYFDYWGQGTLVTVSS 
               
               
                   
               
               
                 61 
                 Pertuzumab 
                 GAGGTGCAATTGGTTGAAAGCGGTGGTGGTCTGGTTCAGCC 
               
               
                   
                 aff.mat. clone 
                 TGGTGGTAGCCTGCGTCTGAGCTGTGCAGCAAGCGGTTTTAC 
               
               
                   
                 D1 DNA 
                 CTTTAACGATTATACCATGGATTGGGTTCGTCAGGCACCGGG 
               
               
                   
                   
                 TAAAGGTCTGGAATGGGTTGCAGATGTTAATCCGAATAGCG 
               
               
                   
                   
                 GTGGTAGCATTTATAACCAGCGTTTTAAAGGTCGTTTTACCC 
               
               
                   
                   
                 TGAGCGTTGATCGTAGCAAAAATACCCTGTATCTGCAAATG 
               
               
                   
                   
                 AATAGTCTGCGTGCAGAGGATACCGCAGTGTATTATTGTGCA 
               
               
                   
                   
                 CGTAACCTGGGTCCGTTCTTCTACTTTGATTATTGGGGTCAG 
               
               
                   
                   
                 GGCACCCTGGTTACCGTTAGCAGC 
               
               
                   
               
               
                 55 
                 Pertuzumab 
                 
                   GFTFNDYTMD 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 D1- V H  CDR1 
                   
               
               
                   
               
               
                 15 
                 Pertuzumab 
                 
                   DVNPNSGGSIYNQRFKG 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 D1- V H  CDR2 
                   
               
               
                   
               
               
                 56 
                 Pertuzumab 
                 
                   NLGPFFYFDY 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 D1- V H  CDR3 
                   
               
               
                   
               
               
                 64 
                 Pertuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFNDYTMDWVRQAPG 
               
               
                   
                 aff.mat. clone 
                 KGLEWVADVNPNSGGSIVNRRFKGRFTLSVDRSKNTLYLQMN 
               
               
                   
                 D1-derived 
                 SLRAEDTAVYYCARNLGPFFYFDYWGQGTLVTVSS 
               
               
                   
               
               
                 63 
                 Pertuzumab 
                 GAGGTGCAATTGGTTGAAAGCGGTGGTGGTCTGGTTCAGCC 
               
               
                   
                 aff.mat. clone 
                 TGGTGGTAGCCTGCGTCTGAGCTGTGCAGCAAGCGGTTTTAC 
               
               
                   
                 D1-derived, 
                 CTTTAACGATTATACCATGGATTGGGTTCGTCAGGCACCGGG 
               
               
                   
                 DNA 
                 TAAAGGTCTGGAATGGGTTGCAGATGTTAATCCGAATAGCG 
               
               
                   
                   
                 GTGGTAGCATTGTTAACCGTCGTTTTAAAGGTCGTTTTACCC 
               
               
                   
                   
                 TGAGCGTTGATCGTAGCAAAAATACCCTGTATCTGCAAATG 
               
               
                   
                   
                 AATAGTCTGCGTGCAGAGGATACCGCAGTGTATTATTGTGCA 
               
               
                   
                   
                 CGTAACCTGGGTCCGTTCTTCTACTTTGATTATTGGGGTCAG 
               
               
                   
                   
                 GGCACCCTGGTTACCGTTAGCAGC 
               
               
                   
               
               
                 55 
                 Pertuzumab 
                 
                   GFTFNDYTMD 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 Di-derived V H   
                   
               
               
                   
                 CDR 1 
                   
               
               
                   
               
               
                 77 
                 Pertuzumab 
                 
                   DVNPNSGGSIVNRRFKG 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 Di-derived V H   
                   
               
               
                   
                 CDR2 
                   
               
               
                   
               
               
                 56 
                 Pertuzumab 
                 
                   NLGPFFYFDY 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 D1- derived V H   
                   
               
               
                   
                 CDR3 
                   
               
               
                   
               
               
                 66 
                 Pertuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFNDYTMDWFRQAPG 
               
               
                   
                 aff.mat. clone 
                 KGLEWVADVNPNSGGSIYNQRFKGRFTLSVDRSKNTLYLQMN 
               
               
                   
                 B2 
                 SLRAEDTAVYYCARNLGPNFYFDYWGQGTLVTVSS 
               
               
                   
               
               
                 65 
                 Pertuzumab 
                 GAGGTGCAATTGGTTGAAAGCGGTGGTGGTCTGGTTCAGCC 
               
               
                   
                 aff.mat. clone 
                 TGGTGGTAGCCTGCGTCTGAGCTGTGCAGCAAGCGGTTTTAC 
               
               
                   
                 B2, DNA 
                 CTTTAACGATTATACCATGGATTGGTTTCGTCAGGCACCGGG 
               
               
                   
                   
                 TAAAGGTCTGGAATGGGTTGCAGATGTTAATCCGAATAGCG 
               
               
                   
                   
                 GTGGTAGCATTTATAACCAGCGTTTTAAAGGTCGTTTTACCC 
               
               
                   
                   
                 TGAGCGTTGATCGTAGCAAAAATACCCTGTATCTGCAAATG 
               
               
                   
                   
                 AATAGTCTGCGTGCAGAGGATACCGCAGTGTATTATTGTGCA 
               
               
                   
                   
                 CGTAATCTGGGTCCGAACTTCTACTTTGATTATTGGGGTCAG 
               
               
                   
                   
                 GGCACCCTGGTTACCGTTAGCAGC 
               
               
                   
               
               
                 55 
                 Pertuzumab 
                 
                   GFTFNDYTMD 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 B2- V H  CDR1 
                   
               
               
                   
               
               
                 15 
                 Pertuzumab 
                 
                   DVNPNSGGSIYNQRFKG 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 B2- V H  CDR2 
                   
               
               
                   
               
               
                 57 
                 Pertuzumab 
                 
                   NLGPNFYFDY 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 B2- V H  CDR3 
                   
               
               
                   
               
               
                 68 
                 Pertuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFADYTMDWVRQAPG 
               
               
                   
                 aff.mat. clone 
                 KGLEWVADVNPNSGGSIYNQRFKGRFTLSVDRSKNTLYLQMN 
               
               
                   
                 E1 
                 SLRAEDTAVYYCARNLGPWFYFDYWGQGTLVTVSS 
               
               
                   
               
               
                 67 
                 Pertuzumab 
                 GAGGTGCAATTGGTTGAAAGCGGTGGTGGTCTGGTTCAGCC 
               
               
                   
                 aff.mat. clone 
                 TGGTGGTAGCCTGCGTCTGAGCTGTGCAGCAAGCGGTTTTAC 
               
               
                   
                 E1, DNA 
                 CTTTGCAGATTATACCATGGATTGGGTTCGTCAGGCACCGGG 
               
               
                   
                   
                 TAAAGGTCTGGAATGGGTTGCAGATGTTAATCCGAATAGCG 
               
               
                   
                   
                 GTGGTAGCATTTATAACCAGCGTTTTAAAGGTCGTTTTACCC 
               
               
                   
                   
                 TGAGCGTTGATCGTAGCAAAAATACCCTGTATCTGCAAATG 
               
               
                   
                   
                 AATAGTCTGCGTGCAGAGGATACCGCAGTGTATTATTGTGCA 
               
               
                   
                   
                 CGTAATCTGGGTCCGTGGTTCTACTTTGATTATTGGGGTCAG 
               
               
                   
                   
                 GGCACCCTGGTTACCGTTAGCAGC 
               
               
                   
               
               
                 58 
                 Pertuzumab 
                 
                   GFTFADYTMD 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 E1- V H  CDR1 
                   
               
               
                   
               
               
                 15 
                 Pertuzumab 
                 
                   DVNPNSGGSIYNQRFKG 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 E1- V H  CDR2 
                   
               
               
                   
               
               
                 59 
                 Pertuzumab 
                 
                   NLGPWFYFDY 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 E1- V H  CDR3 
                   
               
               
                   
               
               
                 70 
                 Pertuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFTDYTMDWVRQAPG 
               
               
                   
                 aff.mat. clone 
                 KGLEWVADVNPNSGGYIVNRRFKGRFTLSVDRSKNTLYLQMN 
               
               
                   
                 G2 
                 SLRAEDTAVYYCARNLGPSFYFDYWGQGTLVTVSS 
               
               
                   
               
               
                 69 
                 Pertuzumab 
                 GAGGTGCAATTGGTTGAAAGCGGTGGTGGTCTGGTTCAGCC 
               
               
                   
                 aff.mat. clone 
                 TGGTGGTAGCCTGCGTCTGAGCTGTGCAGCAAGCGGTTTTAC 
               
               
                   
                 G2, DNA 
                 CTTTACCGATTACACAATGGATTGGGTTCGTCAGGCACCGGG 
               
               
                   
                   
                 TAAAGGTCTGGAATGGGTTGCAGATGTTAATCCGAACTCTG 
               
               
                   
                   
                 GTGGTTACATTGTTAACCGTCGTTTTAAAGGTCGTTTTACCCT 
               
               
                   
                   
                 GAGCGTTGATCGTAGCAAAAATACCCTGTATCTGCAAATGA 
               
               
                   
                   
                 ATAGTCTGCGTGCAGAGGATACCGCAGTGTATTATTGTGCAC 
               
               
                   
                   
                 GTAATCTGGGTCCGAGCTTCTATTTTGATTATTGGGGTCAGG 
               
               
                   
                   
                 GCACCCTGGTTACCGTTAGCAGC 
               
               
                   
               
               
                 14 
                 Pertuzumab 
                 
                   GFTFTDYTMD 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 G2- V H  CDR1 
                   
               
               
                   
               
               
                 60 
                 Pertuzumab 
                 
                   DVNPNSGGYIVNRRFKG 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 G2- V H  CDR2 
                   
               
               
                   
               
               
                 16 
                 Pertuzumab 
                 
                   NLGPSFYFDY 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 G2- V H  CDR3 
                   
               
               
                   
               
               
                 72 
                 Pertuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFTDYTMDWVRQAPG 
               
               
                   
                 aff.mat. clone 
                 KGLEWVADVNPNSGGSIMNRRFKGRFTLSVDRSKNTLYLQMN 
               
               
                   
                 C8 
                 SLRAEDTAVYYCARNLGPSFYFDYWGQGTLVTVSS 
               
               
                   
               
               
                 71 
                 Pertuzumab 
                 GAGGTGCAATTGGTTGAAAGCGGTGGTGGTCTGGTTCAGCC 
               
               
                   
                 aff.mat. clone 
                 TGGTGGTAGCCTGCGTCTGAGCTGTGCAGCAAGCGGTTTTAC 
               
               
                   
                 C8, DNA 
                 CTTTACCGATTACACAATGGATTGGGTTCGTCAGGCACCGGG 
               
               
                   
                   
                 TAAAGGTCTGGAATGGGTTGCAGATGTTAATCCGAACTCTG 
               
               
                   
                   
                 GTGGTTCTATTATGAACCGTCGTTTTAAAGGTCGTTTTACCCT 
               
               
                   
                   
                 GAGCGTTGATCGTAGCAAAAATACCCTGTATCTGCAAATGA 
               
               
                   
                   
                 ATAGTCTGCGTGCAGAGGATACCGCAGTGTATTATTGTGCAC 
               
               
                   
                   
                 GTAATCTGGGTCCGAGCTTCTATTTTGATTATTGGGGTCAGG 
               
               
                   
                   
                 GCACCCTGGTTACCGTTAGCAGC 
               
               
                   
               
               
                 14 
                 Pertuzumab 
                 
                   GFTFTDYTMD 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 C8- V H  CDR1 
                   
               
               
                   
               
               
                 75 
                 Pertuzumab 
                 
                   DVNPNSGGSIMNRRFKG 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 C8- V H  CDR2 
                   
               
               
                   
               
               
                 16 
                 Pertuzumab 
                 
                   NLGPSFYFDY 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 C8- V H  CDR3 
                   
               
               
                   
               
               
                 74 
                 Pertuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFTDYTMDWVRQAPG 
               
               
                   
                 aff.mat. clone 
                 KGLEWVADVNPNSGGSIVNQRFKGRFTLSVDRSKNTLYLQMN 
               
               
                   
                 A1 
                 SLRAEDTAVYYCARNLGPWFYFDYWGQGTLVTVSS 
               
               
                   
               
               
                 73 
                 Pertuzumab 
                 GAGGTGCAATTGGTTGAAAGCGGTGGTGGTCTGGTTCAGCC 
               
               
                   
                 aff.mat. clone 
                 TGGTGGTAGCCTGCGTCTGAGCTGTGCAGCAAGCGGTTTTAC 
               
               
                   
                 A1, DNA 
                 CTTTACCGATTACACAATGGATTGGGTTCGTCAGGCACCGGG 
               
               
                   
                   
                 TAAAGGTCTGGAATGGGTTGCAGATGTTAATCCGAACTCTG 
               
               
                   
                   
                 GTGGTTCTATTGTTAACCAGCGTTTTAAAGGTCGTTTTACCCT 
               
               
                   
                   
                 GAGCGTTGATCGTAGCAAAAATACCCTGTATCTGCAAATGA 
               
               
                   
                   
                 ATAGTCTGCGTGCAGAGGATACCGCAGTGTATTATTGTGCAC 
               
               
                   
                   
                 GTAATCTGGGTCCGTGGTTCTACTTTGATTATTGGGGTCAGG 
               
               
                   
                   
                 GCACCCTGGTTACCGTTAGCAGC 
               
               
                   
               
               
                 14 
                 Pertuzumab 
                 
                   GFTFTDYTMD 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 A1- V H  CDR1 
                   
               
               
                   
               
               
                 76 
                 Pertuzumab 
                 
                   DVNPNSGGSIVNQRFKG 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 A1- V H  CDR2 
                   
               
               
                   
               
               
                 59 
                 Pertuzumab 
                 
                   NLGPWFYFDY 
                 
               
               
                   
                 aff.mat. clone 
                   
               
               
                   
                 A1- V H  CDR3 
                   
               
               
                   
               
            
           
           
               
            
               
                 Trastuzumab Stabilization Variants 
               
            
           
           
               
               
               
            
               
                 84 
                 Trastuzumab 
                 DIQMTQSPSSLSASVGDRVTITCRASQDVN   A   AVAWYQQKPGK 
               
               
                   
                 VL T31A 
                 APKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (6641) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 83 
                 Trastuzumab 
                 GATATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 
               
               
                   
                 VL T31A 
                 GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGA 
               
               
                   
                 (6641) 
                 CGTGAACGCCGCTGTAGCGTGGTACCAGCAGAAACCAGGTA 
               
               
                   
                   
                 AGGCACCGAAGCTATTAATTTATAGTGCGAGCTTCCTGTACA 
               
               
                   
                   
                 GTGGGGTCCCGTCGCGTTTTAGCGGCTCTCGATCCGGCACGG 
               
               
                   
                   
                 ATTTTACCCTGACCATTAGCAGCCTGCAGCCTGAAGACTTTG 
               
               
                   
                   
                 CGACATATTATTGCCAACAGCACTACACAACTCCTCCCACCT 
               
               
                   
                   
                 TTGGCCAGGGTACGAAAGTTGAAATTAAA 
               
               
                   
               
               
                 103 
                 Trastuzumab 
                 
                   RASQDVNAAVA 
                 
               
               
                   
                 VL T31A 
                   
               
               
                   
                 (6641)CDR1 
                   
               
               
                   
               
               
                 18 
                 Trastuzumab 
                 
                   SASFLYS 
                 
               
               
                   
                 VL T31A 
                   
               
               
                   
                 (6641)CDR2 
                   
               
               
                   
               
               
                 19 
                 Trastuzumab 
                 
                   QQHYTTPPT 
                 
               
               
                   
                 VL T31A 
                   
               
               
                   
                 (6641)CDR3 
                   
               
               
                   
               
               
                 86 
                 Trastuzumab 
                 DIQMTQSPSSLSASVGDRVTITCRASQDVN   V   AVAWYQQKPGK 
               
               
                   
                 VL T31V 
                 APKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYC 
               
               
                   
                 (6642) 
                 QQHYTTPPTFGQGTKVEIK 
               
               
                   
               
               
                 85 
                 Trastuzumab 
                 GATATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCT 
               
               
                   
                 VL T31V 
                 GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGA 
               
               
                   
                 (6642)DNA 
                 CGTGAACGTGGCTGTAGCGTGGTACCAGCAGAAACCAGGTA 
               
               
                   
                   
                 AGGCACCGAAGCTATTAATTTATAGTGCGAGCTTCCTGTACA 
               
               
                   
                   
                 GTGGGGTCCCGTCGCGTTTTAGCGGCTCTCGATCCGGCACGG 
               
               
                   
                   
                 ATTTTACCCTGACCATTAGCAGCCTGCAGCCTGAAGACTTTG 
               
               
                   
                   
                 CGACATATTATTGCCAACAGCACTACACAACTCCTCCCACCT 
               
               
                   
                   
                 TTGGCCAGGGTACGAAAGTTGAAATTAAAG 
               
               
                   
               
               
                 104 
                 Trastuzumab 
                 
                   RASQDVNVAVA 
                 
               
               
                   
                 VL T31V 
                   
               
               
                   
                 (6642)CDR1 
                   
               
               
                   
               
               
                 18 
                 Trastuzumab 
                 
                   SASFLYS 
                 
               
               
                   
                 VL T31V 
                   
               
               
                   
                 (6642)CDR2 
                   
               
               
                   
               
               
                 19 
                 Trastuzumab 
                 
                   QQHYTTPPT 
                 
               
               
                   
                 VL T31V 
                   
               
               
                   
                 (6642)CDR3 
                   
               
               
                   
               
               
                 158 
                 Trastuzumab 
                 
                   RASQDVSTAVA 
                 
               
               
                   
                 VL N30S CDR1 
                   
               
               
                   
               
               
                 94 
                 Trastuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYTHWVRQAPGK 
               
               
                   
                 VH (D98N) 
                 GLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSL 
               
               
                   
                 (6636) 
                 RAEDTAVYYCSRWGG   N   GFYAMDYWGQGTLVTVSS 
               
               
                   
               
               
                 93 
                 Trastuzumab 
                 GAAGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACC 
               
               
                   
                 VH (D98N) 
                 GGGCGGCAGCCTGCGTCTGAGCTGCGCGGCCTCCGGATTTA 
               
               
                   
                 (6636)DNA 
                 ACATAAAGGACACATACATCCACTGGGTGCGCCAAGCACCT 
               
               
                   
                   
                 GGGAAGGGTCTCGAGTGGGTGGCTCGGATTTACCCAACAAA 
               
               
                   
                   
                 TGGCTACACCAGGTATGCGGATAGCGTGAAAGGCCGTTTTA 
               
               
                   
                   
                 CCATTTCAGCTGATACTTCGAAGAACACCGCCTATCTGCAAA 
               
               
                   
                   
                 TGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCT 
               
               
                   
                   
                 CGCGTTGGGGAGGAAACGGGTTCTATGCTATGGATTACTGG 
               
               
                   
                   
                 GGCCAAGGCACCCTGGTGACGGTTAGCTCA 
               
               
                   
               
               
                 20 
                 Trastuzumab 
                 
                   GFNIKDTYIH 
                 
               
               
                   
                 VH (D98N) 
                   
               
               
                   
                 (6636)CDR1 
                   
               
               
                   
               
               
                 29 
                 Trastuzumab 
                 
                   RIYPTNGYTRYADSVKG 
                 
               
               
                   
                 VH (D98N) 
                   
               
               
                   
                 (6636)CDR2 
                   
               
               
                   
               
               
                 78 
                 Trastuzumab 
                 
                   WGGNGFYAMDY 
                 
               
               
                   
                 VH (D98N) 
                   
               
               
                   
                 (6636)CDR3 
                   
               
               
                   
               
               
                 96 
                 Trastuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGK 
               
               
                   
                 VH (D98E) 
                 GLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSL 
               
               
                   
                 (6637) 
                 RAEDTAVYYCSRWGG   E   GFYAMDYWGQGTLVTVSS 
               
               
                   
               
               
                 95 
                 Trastuzumab 
                 GAAGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACC 
               
               
                   
                 VH (D98E) 
                 GGGCGGCAGCCTGCGTCTGAGCTGCGCGGCCTCCGGATTTA 
               
               
                   
                 (6637)DNA 
                 ACATAAAGGACACATACATCCACTGGGTGCGCCAAGCACCT 
               
               
                   
                   
                 GGGAAGGGTCTCGAGTGGGTGGCTCGGATTTACCCAACAAA 
               
               
                   
                   
                 TGGCTACACCAGGTATGCGGATAGCGTGAAAGGCCGTTTTA 
               
               
                   
                   
                 CCATTTCAGCTGATACTTCGAAGAACACCGCCTATCTGCAAA 
               
               
                   
                   
                 TGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCT 
               
               
                   
                   
                 CGCGTTGGGGAGGAGAGGGGTTCTATGCTATGGATTACTGG 
               
               
                   
                   
                 GGCCAAGGCACCCTGGTGACGGTTAGCTCA 
               
               
                   
               
               
                 20 
                 Trastuzumab 
                 
                   GFNIKDTYIH 
                 
               
               
                   
                 VH (D98E) 
                   
               
               
                   
                 (6637)CDR1 
                   
               
               
                   
               
               
                 29 
                 Trastuzumab 
                 
                   RIYPTNGYTRYADSVKG 
                 
               
               
                   
                 VH (D98E) 
                   
               
               
                   
                 (6637)CDR2 
                   
               
               
                   
               
               
                 79 
                 Trastuzumab 
                 
                   WGGEGFYAMDY 
                 
               
               
                   
                 VH (D98E) 
                   
               
               
                   
                 (6637)CDR3 
                   
               
               
                   
               
               
                 98 
                 Trastuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGK 
               
               
                   
                 VH (D98T) 
                 GLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSL 
               
               
                   
                 (6638) 
                 RAEDTAVYYCSRWGG T GFYAMDYWGQGTLVTVSS 
               
               
                   
               
               
                 97 
                 Trastuzumab 
                 GAAGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACC 
               
               
                   
                 VH (D98T) 
                 GGGCGGCAGCCTGCGTCTGAGCTGCGCGGCCTCCGGATTTA 
               
               
                   
                 (6638)DNA 
                 ACATAAAGGACACATACATCCACTGGGTGCGCCAAGCACCT 
               
               
                   
                   
                 GGGAAGGGTCTCGAGTGGGTGGCTCGGATTTACCCAACAAA 
               
               
                   
                   
                 TGGCTACACCAGGTATGCGGATAGCGTGAAAGGCCGTTTTA 
               
               
                   
                   
                 CCATTTCAGCTGATACTTCGAAGAACACCGCCTATCTGCAAA 
               
               
                   
                   
                 TGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCT 
               
               
                   
                   
                 CGCGTTGGGGAGGAACCGGGTTCTATGCTATGGATTACTGG 
               
               
                   
                   
                 GGCCAAGGCACCCTGGTGACGGTTAGCTCA 
               
               
                   
               
               
                 20 
                 Trastuzumab 
                 
                   GFNIKDTYIH 
                 
               
               
                   
                 VH (D98T) 
                   
               
               
                   
                 (6638) CDR1 
                   
               
               
                   
               
               
                 29 
                 Trastuzumab 
                 
                   RIYPTNGYTRYADSVKG 
                 
               
               
                   
                 VH (D98T) 
                   
               
               
                   
                 (6638) CDR2 
                   
               
               
                   
               
               
                 80 
                 Trastuzumab 
                 
                   WGGTGFYAMDY 
                 
               
               
                   
                 VH (D98T) 
                   
               
               
                   
                 (6638) CDR3 
                   
               
               
                   
               
               
                 100 
                 Trastuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGK 
               
               
                   
                 VH (G99A) 
                 GLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSL 
               
               
                   
                 (6639) 
                 RAEDTAVYYCSRWGGDAFY   A   MDYWGQGTLVTVSS 
               
               
                   
               
               
                 99 
                 Trastuzumab 
                 GAAGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACC 
               
               
                   
                 VH (G99A) 
                 GGGCGGCAGCCTGCGTCTGAGCTGCGCGGCCTCCGGATTTA 
               
               
                   
                 (6639)DNA 
                 ACATAAAGGACACATACATCCACTGGGTGCGCCAAGCACCT 
               
               
                   
                   
                 GGGAAGGGTCTCGAGTGGGTGGCTCGGATTTACCCAACAAA 
               
               
                   
                   
                 TGGCTACACCAGGTATGCGGATAGCGTGAAAGGCCGTTTTA 
               
               
                   
                   
                 CCATTTCAGCTGATACTTCGAAGAACACCGCCTATCTGCAAA 
               
               
                   
                   
                 TGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCT 
               
               
                   
                   
                 CGCGTTGGGGAGGAGACGCCTTCTATGCTATGGATTACTGG 
               
               
                   
                   
                 GGCCAAGGCACCCTGGTGACGGTTAGCTCA 
               
               
                   
               
               
                 20 
                 Trastuzumab 
                 
                   GFNIKDTYIH 
                 
               
               
                   
                 VH (G99A) 
                   
               
               
                   
                 (6639) CDR1 
                   
               
               
                   
               
               
                 29 
                 Trastuzumab 
                 
                   RIYPTNGYTRYADSVKG 
                 
               
               
                   
                 VH (G99A) 
                   
               
               
                   
                 (6639) CDR2 
                   
               
               
                   
               
               
                 87 
                 Trastuzumab 
                 
                   WGGDAFYAMDY 
                 
               
               
                   
                 VH (G99A) 
                   
               
               
                   
                 (6639) CDR3 
                   
               
               
                   
               
               
                 102 
                 Trastuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGK 
               
               
                   
                 VH (G99S) 
                 GLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSL 
               
               
                   
                 (6640) 
                 RAEDTAVYYCSRWGGD   S   FYAMDYWGQGTLVTVSS 
               
               
                   
               
               
                 101 
                 Trastuzumab 
                 GAAGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACC 
               
               
                   
                 VH (G99S) 
                 GGGCGGCAGCCTGCGTCTGAGCTGCGCGGCCTCCGGATTTA 
               
               
                   
                 (6640)DNA 
                 ACATAAAGGACACATACATCCACTGGGTGCGCCAAGCACCT 
               
               
                   
                   
                 GGGAAGGGTCTCGAGTGGGTGGCTCGGATTTACCCAACAAA 
               
               
                   
                   
                 TGGCTACACCAGGTATGCGGATAGCGTGAAAGGCCGTTTTA 
               
               
                   
                   
                 CCATTTCAGCTGATACTTCGAAGAACACCGCCTATCTGCAAA 
               
               
                   
                   
                 TGAACAGCCTGCGTGCGGAAGATACGGCCGTGTATTATTGCT 
               
               
                   
                   
                 CGCGTTGGGGAGGAGACAGCTTCTATGCTATGGATTACTGG 
               
               
                   
                   
                 GGCCAAGGCACCCTGGTGACGGTTAGCTCA 
               
               
                   
               
               
                 20 
                 Trastuzumab 
                 
                   GFNIKDTYIH 
                 
               
               
                   
                 VH (G99S) 
                   
               
               
                   
                 (6640)CDR1 
                   
               
               
                   
               
               
                 29 
                 Trastuzumab 
                 
                   RIYPTNGYTRYADSVKG 
                 
               
               
                   
                 VH (G99S) 
                   
               
               
                   
                 (6640)CDR2 
                   
               
               
                   
               
               
                 88 
                 Trastuzumab 
                 
                   WGGDSFYAMDY 
                 
               
               
                   
                 VH (G99S) 
                   
               
               
                   
                 (6640)CDR3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 34 
               
             
            
               
                   
               
               
                 Antigens 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO 
                 Name 
                 Sequence 
               
               
                   
               
               
                  2 
                 Her2 ECD 
                 MGWSCIILFLVATATGVHSTQVCTGTDMKLRLPASPETHLDML 
               
               
                   
                   
                 RHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHN 
               
               
                   
                   
                 QVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGA 
               
               
                   
                   
                 SPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKN 
               
               
                   
                   
                 NQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTV 
               
               
                   
                   
                 CAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHS 
               
               
                   
                   
                 GICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLS 
               
               
                   
                   
                 TDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGM 
               
               
                   
                   
                 EHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPL 
               
               
                   
                   
                 QPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILH 
               
               
                   
                   
                 NGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVP 
               
               
                   
                   
                 WDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWG 
               
               
                   
                   
                 PGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHP 
               
               
                   
                   
                 ECQPQNGSVTCFGLEADQCVACAHYKDPPFCVARCPSGVKPDL 
               
               
                   
                   
                 SYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLT 
               
               
                   
                   
                 VDEQLYFQGGSGLNDIFEAQKIEWHEARAHHHHHH 
               
               
                   
               
               
                  1 
                 Her2 ECD DNA 
                 ACCCAAGTGTGCACCGGCACAGACATGAAGCTGCGGCTCCC 
               
               
                   
                   
                 TGCCAGTCCCGAGACCCACCTGGACATGCTCCGCCACCTCTA 
               
               
                   
                   
                 CCAGGGCTGCCAGGTGGTGCAGGGAAACCTGGAACTCACCT 
               
               
                   
                   
                 ACCTGCCCACCAATGCCAGCCTGTCCTTCCTGCAGGATATCC 
               
               
                   
                   
                 AGGAGGTGCAGGGCTACGTGCTCATCGCTCACAACCAAGTG 
               
               
                   
                   
                 AGGCAGGTCCCACTGCAGAGGCTGCGGATTGTGCGAGGCAC 
               
               
                   
                   
                 CCAGCTCTTTGAGGACAACTATGCCCTGGCCGTGCTAGACAA 
               
               
                   
                   
                 TGGAGACCCGCTGAACAATACCACCCCTGTCACAGGGGCCT 
               
               
                   
                   
                 CCCCAGGAGGCCTGCGGGAGCTGCAGCTTCGAAGCCTCACA 
               
               
                   
                   
                 GAGATCTTGAAAGGAGGGGTCTTGATCCAGCGGAACCCCCA 
               
               
                   
                   
                 GCTCTGCTACCAGGACACGATTTTGTGGAAGGACATCTTCCA 
               
               
                   
                   
                 CAAGAACAACCAGCTGGCTCTCACACTGATAGACACCAACC 
               
               
                   
                   
                 GCTCTCGGGCCTGCCACCCCTGTTCTCCGATGTGTAAGGGCT 
               
               
                   
                   
                 CCCGCTGCTGGGGAGAGAGTTCTGAGGATTGTCAGAGCCTG 
               
               
                   
                   
                 ACGCGCACTGTCTGTGCCGGTGGCTGTGCCCGCTGCAAGGG 
               
               
                   
                   
                 GCCACTGCCCACTGACTGCTGCCATGAGCAGTGTGCTGCCGG 
               
               
                   
                   
                 CTGCACGGGCCCCAAGCACTCTGACTGCCTGGCCTGCCTCCA 
               
               
                   
                   
                 CTTCAACCACAGTGGCATCTGTGAGCTGCACTGCCCAGCCCT 
               
               
                   
                   
                 GGTCACCTACAACACAGACACGTTTGAGTCCATGCCCAATCC 
               
               
                   
                   
                 CGAGGGCCGGTATACATTCGGCGCCAGCTGTGTGACTGCCT 
               
               
                   
                   
                 GTCCCTACAACTACCTTTCTACGGACGTGGGATCCTGCACCC 
               
               
                   
                   
                 TCGTCTGCCCCCTGCACAACCAAGAGGTGACAGCAGAGGAT 
               
               
                   
                   
                 GGAACACAGCGGTGTGAGAAGTGCAGCAAGCCCTGTGCCCG 
               
               
                   
                   
                 AGTGTGCTATGGTCTGGGCATGGAGCACTTGCGAGAGGTGA 
               
               
                   
                   
                 GGGCAGTTACCAGTGCCAATATCCAGGAGTTTGCTGGCTGC 
               
               
                   
                   
                 AAGAAGATCTTTGGGAGCCTGGCATTTCTGCCGGAGAGCTTT 
               
               
                   
                   
                 GATGGGGACCCAGCCTCCAACACTGCCCCGCTCCAGCCAGA 
               
               
                   
                   
                 GCAGCTCCAAGTGTTTGAGACTCTGGAAGAGATCACAGGTT 
               
               
                   
                   
                 ACCTATACATCTCAGCATGGCCGGACAGCCTGCCTGACCTCA 
               
               
                   
                   
                 GCGTCTTCCAGAACCTGCAAGTAATCCGGGGACGAATTCTG 
               
               
                   
                   
                 CACAATGGCGCCTACTCGCTGACCCTGCAAGGGCTGGGCAT 
               
               
                   
                   
                 CAGCTGGCTGGGGCTGCGCTCACTGAGGGAACTGGGCAGTG 
               
               
                   
                   
                 GACTGGCCCTCATCCACCATAACACCCACCTCTGCTTCGTGC 
               
               
                   
                   
                 ACACGGTGCCCTGGGACCAGCTCTTTCGGAACCCGCACCAA 
               
               
                   
                   
                 GCTCTGCTCCACACTGCCAACCGGCCAGAGGACGAGTGTGT 
               
               
                   
                   
                 GGGCGAGGGCCTGGCCTGCCACCAGCTGTGCGCCCGAGGGC 
               
               
                   
                   
                 ACTGCTGGGGTCCAGGGCCCACCCAGTGTGTCAACTGCAGC 
               
               
                   
                   
                 CAGTTCCTTCGGGGCCAGGAGTGCGTGGAGGAATGCCGAGT 
               
               
                   
                   
                 ACTGCAGGGGCTCCCCAGGGAGTATGTGAATGCCAGGCACT 
               
               
                   
                   
                 GTTTGCCGTGCCACCCTGAGTGTCAGCCCCAGAATGGCTCAG 
               
               
                   
                   
                 TGACCTGTTTTGGACTGGAGGCTGACCAGTGTGTGGCCTGTG 
               
               
                   
                   
                 CCCACTATAAGGACCCTCCCTTCTGCGTGGCCCGCTGCCCCA 
               
               
                   
                   
                 GCGGTGTGAAACCTGACCTCTCCTACATGCCCATCTGGAAGT 
               
               
                   
                   
                 TTCCAGATGAGGAGGGCGCATGCCAGCCTTGCCCCATCAAC 
               
               
                   
                   
                 TGCACCCACTCCTGTGTGGACCTGGATGACAAGGGCTGCCCC 
               
               
                   
                   
                 GCCGAGCAGAGAGCCAGCCCTCTGACGGTCGACGAACAGTT 
               
               
                   
                   
                 ATATTTTCAGGGCGGCTCAGGCCTGAACGACATCTTCGAGGC 
               
               
                   
                   
                 CCAGAAGATCGAGTGGCACGAGGCTCGAGCTCACCACCATC 
               
               
                   
                   
                 ACCATCAC 
               
               
                   
               
               
                  4 
                 Fc(hole) 
                 DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVV 
               
               
                   
                   
                 DVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL 
               
               
                   
                   
                 TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVC 
               
               
                   
                   
                 TLPPSRDELTKNQVSLSCAVKGFYPSDIAVEWESNGQPENNYK 
               
               
                   
                   
                 TTPPVLDSDGSFFLVSKLTVDKSRWQQGNVFSCSVMHEALHNR 
               
               
                   
                   
                 FTQKSLSLSPGK 
               
               
                   
               
               
                  3 
                 Fc(hole)DNA 
                 GACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACT 
               
               
                   
                   
                 CCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAA 
               
               
                   
                   
                 GGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGT 
               
               
                   
                   
                 GGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCA 
               
               
                   
                   
                 ACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACA 
               
               
                   
                   
                 AAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGT 
               
               
                   
                   
                 CAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCA 
               
               
                   
                   
                 AGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCC 
               
               
                   
                   
                 CCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCG 
               
               
                   
                   
                 AGAACCACAGGTGTGCACCCTGCCCCCATCCCGGGATGAGC 
               
               
                   
                   
                 TGACCAAGAACCAGGTCAGCCTCTCGTGCGCAGTCAAAGGC 
               
               
                   
                   
                 TTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGG 
               
               
                   
                   
                 GCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGG 
               
               
                   
                   
                 ACTCCGACGGCTCCTTCTTCCTCGTGAGCAAGCTCACCGTGG 
               
               
                   
                   
                 ACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCC 
               
               
                   
                   
                 GTGATGCATGAGGCTCTGCACAACCGCTTCACGCAGAAGAG 
               
               
                   
                   
                 CCTCTCCCTGTCTCCGGGTAAA 
               
               
                   
               
               
                  6 
                 Her2 ECD- 
                 TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTY 
               
               
                   
                 Fc(knob) 
                 LPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLF 
               
               
                   
                   
                 EDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKG 
               
               
                   
                   
                 GVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPC 
               
               
                   
                   
                 SPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHE 
               
               
                   
                   
                 QCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFES 
               
               
                   
                   
                 MPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTA 
               
               
                   
                   
                 EDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAG 
               
               
                   
                   
                 CKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYI 
               
               
                   
                   
                 SAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLR 
               
               
                   
                   
                 SLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANR 
               
               
                   
                   
                 PEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECV 
               
               
                   
                   
                 EECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGLEADQC 
               
               
                   
                   
                 VACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCP 
               
               
                   
                   
                 INCTHSCVDLDDKGCPAEQRASPLTVDGGSPTPPTPGGGSADKT 
               
               
                   
                   
                 HTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSH 
               
               
                   
                   
                 EDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLH 
               
               
                   
                   
                 QDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPP 
               
               
                   
                   
                 CRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPP 
               
               
                   
                   
                 VLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQ 
               
               
                   
                   
                 KSLSLSPGKSGGLNDIFEAQKIEWHE 
               
               
                   
               
               
                  5 
                 Her2 ECD- 
                 ACCCAAGTGTGCACCGGCACAGACATGAAGCTGCGGCTCCC 
               
               
                   
                 Fc(knob)DNA 
                 TGCCAGTCCCGAGACCCACCTGGACATGCTCCGCCACCTCTA 
               
               
                   
                   
                 CCAGGGCTGCCAGGTGGTGCAGGGAAACCTGGAACTCACCT 
               
               
                   
                   
                 ACCTGCCCACCAATGCCAGCCTGTCCTTCCTGCAGGATATCC 
               
               
                   
                   
                 AGGAGGTGCAGGGCTACGTGCTCATCGCTCACAACCAAGTG 
               
               
                   
                   
                 AGGCAGGTCCCACTGCAGAGGCTGCGGATTGTGCGAGGCAC 
               
               
                   
                   
                 CCAGCTCTTTGAGGACAACTATGCCCTGGCCGTGCTAGACAA 
               
               
                   
                   
                 TGGAGACCCGCTGAACAATACCACCCCTGTCACAGGGGCCT 
               
               
                   
                   
                 CCCCAGGAGGCCTGCGGGAGCTGCAGCTTCGAAGCCTCACA 
               
               
                   
                   
                 GAGATCTTGAAAGGAGGGGTCTTGATCCAGCGGAACCCCCA 
               
               
                   
                   
                 GCTCTGCTACCAGGACACGATTTTGTGGAAGGACATCTTCCA 
               
               
                   
                   
                 CAAGAACAACCAGCTGGCTCTCACACTGATAGACACCAACC 
               
               
                   
                   
                 GCTCTCGGGCCTGCCACCCCTGTTCTCCGATGTGTAAGGGCT 
               
               
                   
                   
                 CCCGCTGCTGGGGAGAGAGTTCTGAGGATTGTCAGAGCCTG 
               
               
                   
                   
                 ACGCGCACTGTCTGTGCCGGTGGCTGTGCCCGCTGCAAGGG 
               
               
                   
                   
                 GCCACTGCCCACTGACTGCTGCCATGAGCAGTGTGCTGCCGG 
               
               
                   
                   
                 CTGCACGGGCCCCAAGCACTCTGACTGCCTGGCCTGCCTCCA 
               
               
                   
                   
                 CTTCAACCACAGTGGCATCTGTGAGCTGCACTGCCCAGCCCT 
               
               
                   
                   
                 GGTCACCTACAACACAGACACGTTTGAGTCCATGCCCAATCC 
               
               
                   
                   
                 CGAGGGCCGGTATACATTCGGCGCCAGCTGTGTGACTGCCT 
               
               
                   
                   
                 GTCCCTACAACTACCTTTCTACGGACGTGGGATCCTGCACCC 
               
               
                   
                   
                 TCGTCTGCCCCCTGCACAACCAAGAGGTGACAGCAGAGGAT 
               
               
                   
                   
                 GGAACACAGCGGTGTGAGAAGTGCAGCAAGCCCTGTGCCCG 
               
               
                   
                   
                 AGTGTGCTATGGTCTGGGCATGGAGCACTTGCGAGAGGTGA 
               
               
                   
                   
                 GGGCAGTTACCAGTGCCAATATCCAGGAGTTTGCTGGCTGC 
               
               
                   
                   
                 AAGAAGATCTTTGGGAGCCTGGCATTTCTGCCGGAGAGCTTT 
               
               
                   
                   
                 GATGGGGACCCAGCCTCCAACACTGCCCCGCTCCAGCCAGA 
               
               
                   
                   
                 GCAGCTCCAAGTGTTTGAGACTCTGGAAGAGATCACAGGTT 
               
               
                   
                   
                 ACCTATACATCTCAGCATGGCCGGACAGCCTGCCTGACCTCA 
               
               
                   
                   
                 GCGTCTTCCAGAACCTGCAAGTAATCCGGGGACGAATTCTG 
               
               
                   
                   
                 CACAATGGCGCCTACTCGCTGACCCTGCAAGGGCTGGGCAT 
               
               
                   
                   
                 CAGCTGGCTGGGGCTGCGCTCACTGAGGGAACTGGGCAGTG 
               
               
                   
                   
                 GACTGGCCCTCATCCACCATAACACCCACCTCTGCTTCGTGC 
               
               
                   
                   
                 ACACGGTGCCCTGGGACCAGCTCTTTCGGAACCCGCACCAA 
               
               
                   
                   
                 GCTCTGCTCCACACTGCCAACCGGCCAGAGGACGAGTGTGT 
               
               
                   
                   
                 GGGCGAGGGCCTGGCCTGCCACCAGCTGTGCGCCCGAGGGC 
               
               
                   
                   
                 ACTGCTGGGGTCCAGGGCCCACCCAGTGTGTCAACTGCAGC 
               
               
                   
                   
                 CAGTTCCTTCGGGGCCAGGAGTGCGTGGAGGAATGCCGAGT 
               
               
                   
                   
                 ACTGCAGGGGCTCCCCAGGGAGTATGTGAATGCCAGGCACT 
               
               
                   
                   
                 GTTTGCCGTGCCACCCTGAGTGTCAGCCCCAGAATGGCTCAG 
               
               
                   
                   
                 TGACCTGTTTTGGACTGGAGGCTGACCAGTGTGTGGCCTGTG 
               
               
                   
                   
                 CCCACTATAAGGACCCTCCCTTCTGCGTGGCCCGCTGCCCCA 
               
               
                   
                   
                 GCGGTGTGAAACCTGACCTCTCCTACATGCCCATCTGGAAGT 
               
               
                   
                   
                 TTCCAGATGAGGAGGGCGCATGCCAGCCTTGCCCCATCAAC 
               
               
                   
                   
                 TGCACCCACTCCTGTGTGGACCTGGATGACAAGGGCTGCCCC 
               
               
                   
                   
                 GCCGAGCAGAGAGCCAGCCCTCTGACGGTCGACGGTGGTAG 
               
               
                   
                   
                 TCCGACACCTCCGACACCCGGGGGTGGTTCTGCAGACAAAA 
               
               
                   
                   
                 CTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGG 
               
               
                   
                   
                 GGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC 
               
               
                   
                   
                 CTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTG 
               
               
                   
                   
                 GACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTA 
               
               
                   
                   
                 CGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGC 
               
               
                   
                   
                 GGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC 
               
               
                   
                   
                 CTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTA 
               
               
                   
                   
                 CAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCG 
               
               
                   
                   
                 AGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCA 
               
               
                   
                   
                 CAGGTGTACACCCTGCCCCCATGCCGGGATGAGCTGACCAA 
               
               
                   
                   
                 GAACCAGGTCAGCCTGTGGTGCCTGGTCAAAGGCTTCTATCC 
               
               
                   
                   
                 CAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGG 
               
               
                   
                   
                 AGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGAC 
               
               
                   
                   
                 GGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGC 
               
               
                   
                   
                 AGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCA 
               
               
                   
                   
                 TGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCC 
               
               
                   
                   
                 TGTCTCCGGGTAAATCCGGAGGCCTGAACGACATCTTCGAG 
               
               
                   
                   
                 GCCCAGAAGATTGAATGGCACGAG 
               
               
                   
               
               
                  8 
                 Her2 
                 TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTY 
               
               
                   
                 ECD(pertuzumab 
                 LPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLF 
               
               
                   
                 KO)-Fc(knob) 
                 EDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKG 
               
               
                   
                   
                 GVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPC 
               
               
                   
                   
                 SPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHE 
               
               
                   
                   
                 QCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTRES 
               
               
                   
                   
                 MPNPEGRYRFGASCVTACPYNYLSTDRGSCTLVCPLANQEVTA 
               
               
                   
                   
                 EDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAG 
               
               
                   
                   
                 CKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYI 
               
               
                   
                   
                 SAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLR 
               
               
                   
                   
                 SLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANR 
               
               
                   
                   
                 PEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECV 
               
               
                   
                   
                 EECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGLEADQC 
               
               
                   
                   
                 VACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCP 
               
               
                   
                   
                 INCTHSCVDLDDKGCPAEQRASPLTVDGGSPTPPTPGGGSADKT 
               
               
                   
                   
                 HTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSH 
               
               
                   
                   
                 EDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLH 
               
               
                   
                   
                 QDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPP 
               
               
                   
                   
                 CRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPP 
               
               
                   
                   
                 VLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQ 
               
               
                   
                   
                 KSLSLSPGKSGGLNDIFEAQKIEWHE 
               
               
                   
               
               
                  7 
                 Her2 
                 ACCCAAGTGTGCACCGGCACAGACATGAAGCTGCGGCTCCC 
               
               
                   
                 ECD(pertuzumab 
                 TGCCAGTCCCGAGACCCACCTGGACATGCTCCGCCACCTCTA 
               
               
                   
                 KO)- 
                 CCAGGGCTGCCAGGTGGTGCAGGGAAACCTGGAACTCACCT 
               
               
                   
                 Fc(knob)DNA 
                 ACCTGCCCACCAATGCCAGCCTGTCCTTCCTGCAGGATATCC 
               
               
                   
                   
                 AGGAGGTGCAGGGCTACGTGCTCATCGCTCACAACCAAGTG 
               
               
                   
                   
                 AGGCAGGTCCCACTGCAGAGGCTGCGGATTGTGCGAGGCAC 
               
               
                   
                   
                 CCAGCTCTTTGAGGACAACTATGCCCTGGCCGTGCTAGACAA 
               
               
                   
                   
                 TGGAGACCCGCTGAACAATACCACCCCTGTCACAGGGGCCT 
               
               
                   
                   
                 CCCCAGGAGGCCTGCGGGAGCTGCAGCTTCGAAGCCTCACA 
               
               
                   
                   
                 GAGATCTTGAAAGGAGGGGTCTTGATCCAGCGGAACCCCCA 
               
               
                   
                   
                 GCTCTGCTACCAGGACACGATTTTGTGGAAGGACATCTTCCA 
               
               
                   
                   
                 CAAGAACAACCAGCTGGCTCTCACACTGATAGACACCAACC 
               
               
                   
                   
                 GCTCTCGGGCCTGCCACCCCTGTTCTCCGATGTGTAAGGGCT 
               
               
                   
                   
                 CCCGCTGCTGGGGAGAGAGTTCTGAGGATTGTCAGAGCCTG 
               
               
                   
                   
                 ACGCGCACTGTCTGTGCCGGTGGCTGTGCCCGCTGCAAGGG 
               
               
                   
                   
                 GCCACTGCCCACTGACTGCTGCCATGAGCAGTGTGCTGCCGG 
               
               
                   
                   
                 CTGCACGGGCCCCAAGCACTCTGACTGCCTGGCCTGCCTCCA 
               
               
                   
                   
                 CTTCAACCACAGTGGCATCTGTGAGCTGCACTGCCCAGCCCT 
               
               
                   
                   
                 GGTCACCTACAACACAGACACGCGGGAGTCCATGCCCAATC 
               
               
                   
                   
                 CCGAGGGCCGGTATAGATTCGGCGCCAGCTGTGTGACTGCC 
               
               
                   
                   
                 TGTCCCTACAACTACCTTTCTACGGACCGGGGATCCTGCACC 
               
               
                   
                   
                 CTCGTCTGCCCCCTGGCCAACCAAGAGGTGACAGCAGAGGA 
               
               
                   
                   
                 TGGAACACAGCGGTGTGAGAAGTGCAGCAAGCCCTGTGCCC 
               
               
                   
                   
                 GAGTGTGCTATGGTCTGGGCATGGAGCACTTGCGAGAGGTG 
               
               
                   
                   
                 AGGGCAGTTACCAGTGCCAATATCCAGGAGTTTGCTGGCTG 
               
               
                   
                   
                 CAAGAAGATCTTTGGGAGCCTGGCATTTCTGCCGGAGAGCTT 
               
               
                   
                   
                 TGATGGGGACCCAGCCTCCAACACTGCCCCGCTCCAGCCAG 
               
               
                   
                   
                 AGCAGCTCCAAGTGTTTGAGACTCTGGAAGAGATCACAGGT 
               
               
                   
                   
                 TACCTATACATCTCAGCATGGCCGGACAGCCTGCCTGACCTC 
               
               
                   
                   
                 AGCGTCTTCCAGAACCTGCAAGTAATCCGGGGACGAATTCT 
               
               
                   
                   
                 GCACAATGGCGCCTACTCGCTGACCCTGCAAGGGCTGGGCA 
               
               
                   
                   
                 TCAGCTGGCTGGGGCTGCGCTCACTGAGGGAACTGGGCAGT 
               
               
                   
                   
                 GGACTGGCCCTCATCCACCATAACACCCACCTCTGCTTCGTG 
               
               
                   
                   
                 CACACGGTGCCCTGGGACCAGCTCTTTCGGAACCCGCACCA 
               
               
                   
                   
                 AGCTCTGCTCCACACTGCCAACCGGCCAGAGGACGAGTGTG 
               
               
                   
                   
                 TGGGCGAGGGCCTGGCCTGCCACCAGCTGTGCGCCCGAGGG 
               
               
                   
                   
                 CACTGCTGGGGTCCAGGGCCCACCCAGTGTGTCAACTGCAG 
               
               
                   
                   
                 CCAGTTCCTTCGGGGCCAGGAGTGCGTGGAGGAATGCCGAG 
               
               
                   
                   
                 TACTGCAGGGGCTCCCCAGGGAGTATGTGAATGCCAGGCAC 
               
               
                   
                   
                 TGTTTGCCGTGCCACCCTGAGTGTCAGCCCCAGAATGGCTCA 
               
               
                   
                   
                 GTGACCTGTTTTGGACTGGAGGCTGACCAGTGTGTGGCCTGT 
               
               
                   
                   
                 GCCCACTATAAGGACCCTCCCTTCTGCGTGGCCCGCTGCCCC 
               
               
                   
                   
                 AGCGGTGTGAAACCTGACCTCTCCTACATGCCCATCTGGAAG 
               
               
                   
                   
                 TTTCCAGATGAGGAGGGCGCATGCCAGCCTTGCCCCATCAA 
               
               
                   
                   
                 CTGCACCCACTCCTGTGTGGACCTGGATGACAAGGGCTGCCC 
               
               
                   
                   
                 CGCCGAGCAGAGAGCCAGCCCTCTGACGGTCGACGGTGGTA 
               
               
                   
                   
                 GTCCGACACCTCCGACACCCGGGGGTGGTTCTGCAGACAAA 
               
               
                   
                   
                 ACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGG 
               
               
                   
                   
                 GGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC 
               
               
                   
                   
                 CCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGT 
               
               
                   
                   
                 GGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGT 
               
               
                   
                   
                 ACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCG 
               
               
                   
                   
                 CGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGT 
               
               
                   
                   
                 CCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGT 
               
               
                   
                   
                 ACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATC 
               
               
                   
                   
                 GAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACC 
               
               
                   
                   
                 ACAGGTGTACACCCTGCCCCCATGCCGGGATGAGCTGACCA 
               
               
                   
                   
                 AGAACCAGGTCAGCCTGTGGTGCCTGGTCAAAGGCTTCTATC 
               
               
                   
                   
                 CCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCG 
               
               
                   
                   
                 GAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGA 
               
               
                   
                   
                 CGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG 
               
               
                   
                   
                 CAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC 
               
               
                   
                   
                 ATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCC 
               
               
                   
                   
                 CTGTCTCCGGGTAAATCCGGAGGCCTGAACGACATCTTCGA 
               
               
                   
                   
                 GGCCCAGAAGATTGAATGGCACGAG 
               
               
                   
               
               
                 10 
                 Her2 
                 TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTY 
               
               
                   
                 ECD(trastuzumab 
                 LPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLF 
               
               
                   
                 KO)- 
                 EDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKG 
               
               
                   
                 Fc(knob) 
                 GVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPC 
               
               
                   
                   
                 SPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHE 
               
               
                   
                   
                 QCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFES 
               
               
                   
                   
                 MPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTA 
               
               
                   
                   
                 EDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAG 
               
               
                   
                   
                 CKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYI 
               
               
                   
                   
                 SAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLR 
               
               
                   
                   
                 SLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANR 
               
               
                   
                   
                 PEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECV 
               
               
                   
                   
                 EECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGLEARQC 
               
               
                   
                   
                 VACAHYKDRRCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPI 
               
               
                   
                   
                 NCTHSCVDLDDKGCPAEQRASPLTVDGGSPTPPTPGGGSADKT 
               
               
                   
                   
                 HTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSH 
               
               
                   
                   
                 EDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLH 
               
               
                   
                   
                 QDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPP 
               
               
                   
                   
                 CRDELTKNQVSLWCLVKGFYPSDIAVEWESNGQPENNYKTTPP 
               
               
                   
                   
                 VLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQ 
               
               
                   
                   
                 KSLSLSPGKSGGLNDIFEAQKIEWHE 
               
               
                   
               
               
                  9 
                 Her2 
                 ACCCAAGTGTGCACCGGCACAGACATGAAGCTGCGGCTCCC 
               
               
                   
                 ECD(trastuzumab 
                 TGCCAGTCCCGAGACCCACCTGGACATGCTCCGCCACCTCTA 
               
               
                   
                 KO)- 
                 CCAGGGCTGCCAGGTGGTGCAGGGAAACCTGGAACTCACCT 
               
               
                   
                 Fc(knob)DNA 
                 ACCTGCCCACCAATGCCAGCCTGTCCTTCCTGCAGGATATCC 
               
               
                   
                   
                 AGGAGGTGCAGGGCTACGTGCTCATCGCTCACAACCAAGTG 
               
               
                   
                   
                 AGGCAGGTCCCACTGCAGAGGCTGCGGATTGTGCGAGGCAC 
               
               
                   
                   
                 CCAGCTCTTTGAGGACAACTATGCCCTGGCCGTGCTAGACAA 
               
               
                   
                   
                 TGGAGACCCGCTGAACAATACCACCCCTGTCACAGGGGCCT 
               
               
                   
                   
                 CCCCAGGAGGCCTGCGGGAGCTGCAGCTTCGAAGCCTCACA 
               
               
                   
                   
                 GAGATCTTGAAAGGAGGGGTCTTGATCCAGCGGAACCCCCA 
               
               
                   
                   
                 GCTCTGCTACCAGGACACGATTTTGTGGAAGGACATCTTCCA 
               
               
                   
                   
                 CAAGAACAACCAGCTGGCTCTCACACTGATAGACACCAACC 
               
               
                   
                   
                 GCTCTCGGGCCTGCCACCCCTGTTCTCCGATGTGTAAGGGCT 
               
               
                   
                   
                 CCCGCTGCTGGGGAGAGAGTTCTGAGGATTGTCAGAGCCTG 
               
               
                   
                   
                 ACGCGCACTGTCTGTGCCGGTGGCTGTGCCCGCTGCAAGGG 
               
               
                   
                   
                 GCCACTGCCCACTGACTGCTGCCATGAGCAGTGTGCTGCCGG 
               
               
                   
                   
                 CTGCACGGGCCCCAAGCACTCTGACTGCCTGGCCTGCCTCCA 
               
               
                   
                   
                 CTTCAACCACAGTGGCATCTGTGAGCTGCACTGCCCAGCCCT 
               
               
                   
                   
                 GGTCACCTACAACACAGACACGTTTGAGTCCATGCCCAATCC 
               
               
                   
                   
                 CGAGGGCCGGTATACATTCGGCGCCAGCTGTGTGACTGCCT 
               
               
                   
                   
                 GTCCCTACAACTACCTTTCTACGGACGTGGGATCCTGCACCC 
               
               
                   
                   
                 TCGTCTGCCCCCTGCACAACCAAGAGGTGACAGCAGAGGAT 
               
               
                   
                   
                 GGAACACAGCGGTGTGAGAAGTGCAGCAAGCCCTGTGCCCG 
               
               
                   
                   
                 AGTGTGCTATGGTCTGGGCATGGAGCACTTGCGAGAGGTGA 
               
               
                   
                   
                 GGGCAGTTACCAGTGCCAATATCCAGGAGTTTGCTGGCTGC 
               
               
                   
                   
                 AAGAAGATCTTTGGGAGCCTGGCATTTCTGCCGGAGAGCTTT 
               
               
                   
                   
                 GATGGGGACCCAGCCTCCAACACTGCCCCGCTCCAGCCAGA 
               
               
                   
                   
                 GCAGCTCCAAGTGTTTGAGACTCTGGAAGAGATCACAGGTT 
               
               
                   
                   
                 ACCTATACATCTCAGCATGGCCGGACAGCCTGCCTGACCTCA 
               
               
                   
                   
                 GCGTCTTCCAGAACCTGCAAGTAATCCGGGGACGAATTCTG 
               
               
                   
                   
                 CACAATGGCGCCTACTCGCTGACCCTGCAAGGGCTGGGCAT 
               
               
                   
                   
                 CAGCTGGCTGGGGCTGCGCTCACTGAGGGAACTGGGCAGTG 
               
               
                   
                   
                 GACTGGCCCTCATCCACCATAACACCCACCTCTGCTTCGTGC 
               
               
                   
                   
                 ACACGGTGCCCTGGGACCAGCTCTTTCGGAACCCGCACCAA 
               
               
                   
                   
                 GCTCTGCTCCACACTGCCAACCGGCCAGAGGACGAGTGTGT 
               
               
                   
                   
                 GGGCGAGGGCCTGGCCTGCCACCAGCTGTGCGCCCGAGGGC 
               
               
                   
                   
                 ACTGCTGGGGTCCAGGGCCCACCCAGTGTGTCAACTGCAGC 
               
               
                   
                   
                 CAGTTCCTTCGGGGCCAGGAGTGCGTGGAGGAATGCCGAGT 
               
               
                   
                   
                 ACTGCAGGGGCTCCCCAGGGAGTATGTGAATGCCAGGCACT 
               
               
                   
                   
                 GTTTGCCGTGCCACCCTGAGTGTCAGCCCCAGAATGGCTCAG 
               
               
                   
                   
                 TGACCTGTTTTGGACTGGAGGCTCGGCAGTGTGTGGCCTGTG 
               
               
                   
                   
                 CCCACTATAAGGACAGACGGTGCGTGGCCCGCTGCCCCAGC 
               
               
                   
                   
                 GGTGTGAAACCTGACCTCTCCTACATGCCCATCTGGAAGTTT 
               
               
                   
                   
                 CCAGATGAGGAGGGCGCATGCCAGCCTTGCCCCATCAACTG 
               
               
                   
                   
                 CACCCACTCCTGTGTGGACCTGGATGACAAGGGCTGCCCCG 
               
               
                   
                   
                 CCGAGCAGAGAGCCAGCCCTCTGACGGTCGACGGTGGTAGT 
               
               
                   
                   
                 CCGACACCTCCGACACCCGGGGGTGGTTCTGCAGACAAAAC 
               
               
                   
                   
                 TCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGG 
               
               
                   
                   
                 GACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCC 
               
               
                   
                   
                 TCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGG 
               
               
                   
                   
                 ACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTAC 
               
               
                   
                   
                 GTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCG 
               
               
                   
                   
                 GGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCC 
               
               
                   
                   
                 TCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTAC 
               
               
                   
                   
                 AAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGA 
               
               
                   
                   
                 GAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCAC 
               
               
                   
                   
                 AGGTGTACACCCTGCCCCCATGCCGGGATGAGCTGACCAAG 
               
               
                   
                   
                 AACCAGGTCAGCCTGTGGTGCCTGGTCAAAGGCTTCTATCCC 
               
               
                   
                   
                 AGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGG 
               
               
                   
                   
                 AGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGAC 
               
               
                   
                   
                 GGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGC 
               
               
                   
                   
                 AGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCA 
               
               
                   
                   
                 TGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCC 
               
               
                   
                   
                 TGTCTCCGGGTAAATCCGGAGGCCTGAACGACATCTTCGAG 
               
               
                   
                   
                 GCCCAGAAGATTGAATGGCACGAG 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 35 
               
             
            
               
                   
               
               
                 Full-length antibody sequences of common light chain antibody ″D1 der″ 
               
            
           
           
               
               
               
            
               
                 SEQ ID 
                   
                   
               
               
                 NO 
                 Name 
                 Sequence 
               
               
                   
               
               
                 159 
                 Trastuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGK 
               
               
                   
                 VHCH1-Fc 
                 GLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSL 
               
               
                   
                 KNOB 
                 RAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPS 
               
               
                   
                   
                 VFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGV 
               
               
                   
                   
                 HTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKV 
               
               
                   
                   
                 DKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRT 
               
               
                   
                   
                 PEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNS 
               
               
                   
                   
                 TYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKG 
               
               
                   
                   
                 QPREPQVYTLPPCRDELTKNQVSLWCLVKGFYPSDIAVEWESN 
               
               
                   
                   
                 GQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSV 
               
               
                   
                   
                 MHEALHNHYTQKSLSLSPGK 
               
               
                   
               
               
                 160 
                 Trastuzumab 
                 GAAGTGCAATTGGTGGAAAGCGGCGGCGGCCTGGTGCAACCGGG 
               
               
                   
                 VHCH1-Fc 
                 CGGCAGCCTGCGTCTGAGCTGCGCGGCCTCCGGATTTAACATAAA 
               
               
                   
                 KNOB DNA 
                 GGACACATACATCCACTGGGTGCGCCAAGCACCTGGGAAGGGTCT 
               
               
                   
                   
                 CGAGTGGGTGGCTCGGATTTACCCAACAAATGGCTACACCAGGTA 
               
               
                   
                   
                 TGCGGATAGCGTGAAAGGCCGTTTTACCATTTCAGCTGATACTTCG 
               
               
                   
                   
                 AAGAACACCGCCTATCTGCAAATGAACAGCCTGCGTGCGGAAGAT 
               
               
                   
                   
                 ACGGCCGTGTATTATTGCTCGCGTTGGGGAGGAGACGGGTTCTAT 
               
               
                   
                   
                 GCTATGGATTACTGGGGCCAAGGCACCCTGGTGACGGTTAGCTCA 
               
               
                   
                   
                 GCTAGCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCA 
               
               
                   
                   
                 AGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGG 
               
               
                   
                   
                 ACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCC 
               
               
                   
                   
                 TGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGG 
               
               
                   
                   
                 ACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTG 
               
               
                   
                   
                 GGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC 
               
               
                   
                   
                 ACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACT 
               
               
                   
                   
                 CACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCG 
               
               
                   
                   
                 TCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCT 
               
               
                   
                   
                 CCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACG 
               
               
                   
                   
                 AAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGG 
               
               
                   
                   
                 TGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGC 
               
               
                   
                   
                 ACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGG 
               
               
                   
                   
                 CTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTC 
               
               
                   
                   
                 CCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCC 
               
               
                   
                   
                 CGAGAACCACAGGTGTACACCCTGCCCCCATGCCGGGATGAGCTG 
               
               
                   
                   
                 ACCAAGAACCAGGTCAGCCTGTGGTGCCTGGTCAAAGGCTTCTAT 
               
               
                   
                   
                 CCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA 
               
               
                   
                   
                 GAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTC 
               
               
                   
                   
                 CTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCA 
               
               
                   
                   
                 GCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCA 
               
               
                   
                   
                 CAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA 
               
               
                   
               
               
                 161 
                 Common light 
                 DIQMTQSPSSLSASVGDRVTITCKASQDVSTAVAWYQQKPGKAPKLL 
               
               
                   
                 chain VLCL 
                 IYSASFRYTGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPT 
               
               
                   
                   
                 FGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAK 
               
               
                   
                   
                 VQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKV 
               
               
                   
                   
                 YACEVTHQGLSSPVTKSFNRGEC 
               
               
                   
               
               
                 162 
                 Common light 
                 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGTCTGCCAGCGTGGGCG 
               
               
                   
                 chain VLCL- 
                 ACAGAGTGACCATCACATGCAAGGCCAGCCAGGACGTGTCCACAGCCGT 
               
               
                   
                 DNA 
                 GGCCTGGTATCAGCAGAAGCCTGGCAAGGCCCCCAAGCTGCTGATCTACA 
               
               
                   
                   
                 GCGCCAGCTTCCGGTACACCGGCGTGCCCAGCAGATTCAGCGGCAGCAG 
               
               
                   
                   
                 ATCCGGCACCGACTTCACCCTGACCATCAGCTCCCTGCAGCCCGAGGACT 
               
               
                   
                   
                 TCGCCACCTACTACTGCCAGCAGCACTACACCACCCCCCCCACATTTGGC 
               
               
                   
                   
                 CAGGGCACCAAGGTGGAAATCAAGCGTACGGTGGCTGCACCATCTGTCTT 
               
               
                   
                   
                 CATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT 
               
               
                   
                   
                 GTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAG 
               
               
                   
                   
                 GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGC 
               
               
                   
                   
                 AGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAG 
               
               
                   
                   
                 CAAAGCAGACTACGAGAAACACAAAGTCTACGccTGCGAAGTCACCCAT 
               
               
                   
                   
                 CAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT 
               
               
                   
               
               
                 163 
                 Pertuzumab 
                 EVQLVESGGGLVQPGGSLRLSCAASGFTFNDYTMDWVRQAPGKGLE 
               
               
                   
                 VHCH1 Fc 
                 WVADVNPNSGGSIVNRRFKGRFTLSVDRSKNTLYLQMNSLRAEDTA 
               
               
                   
                 hole 
                 VYYCARNLGPFFYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSG 
               
               
                   
                   
                 GTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSS 
               
               
                   
                   
                 VVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAP 
               
               
                   
                   
                 ELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYV 
               
               
                   
                   
                 DGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVS 
               
               
                   
                   
                 NKALPAPIEKTISKAKGQPREPQVCTLPPSRDELTKNQVSLSCAVKGF 
               
               
                   
                   
                 YPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLVSKLTVDKSRWQQ 
               
               
                   
                   
                 GNVFSCSVMHEALHNHYTQKSLSLSPGK 
               
               
                   
               
               
                 164 
                 Pertuzumab 
                 GAAGTTCAGCTGGTTGAAAGCGGTGGTGGTCTGGTTCAGCCTGGT 
               
               
                   
                 VHCH1 Fc 
                 GGTAGCCTGCGTCTGAGCTGTGCAGCAAGCGGTTTTACCTTTAACG 
               
               
                   
                 hole DNA 
                 ATTATACCATGGATTGGGTTCGTCAGGCACCGGGTAAAGGTCTGG 
               
               
                   
                   
                 AATGGGTTGCAGATGTTAATCCGAATAGCGGTGGTAGCATTGTTA 
               
               
                   
                   
                 ACCGTCGTTTTAAAGGTCGTTTTACCCTGAGCGTTGATCGTAGCAA 
               
               
                   
                   
                 AAATACCCTGTATCTGCAAATGAATAGTCTGCGTGCAGAGGATAC 
               
               
                   
                   
                 CGCAGTGTATTATTGTGCACGTAACCTGGGTCCGTTCTTCTACTTT 
               
               
                   
                   
                 GATTATTGGGGTCAGGGCACCCTGGTTACCGTTAGCAGCGCTAGC 
               
               
                   
                   
                 ACCAAGGGCCCAAGCGTGTTCCCTCTGGCCCCCAGCAGCAAGAGC 
               
               
                   
                   
                 ACAAGCGGCGGAACAGCCGCCCTGGGCTGCCTGGTCAAGGACTAC 
               
               
                   
                   
                 TTCCCCGAGCCCGTGACAGTGTCCTGGAACAGCGGAGCCCTGACC 
               
               
                   
                   
                 AGCGGCGTGCACACCTTTCCAGCCGTGCTGCAGAGCAGCGGCCTG 
               
               
                   
                   
                 TACAGCCTGAGCAGCGTGGTCACAGTGCCTAGCAGCAGCCTGGGC 
               
               
                   
                   
                 ACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACC 
               
               
                   
                   
                 AAGGTGGACAAGAAGGTGGAGCCCAAGAGCTGCGACAAGACCCA 
               
               
                   
                   
                 CACCTGTCCCCCTTGTCCTGCCCCTGAGCTGCTGGGCGGACCCAGC 
               
               
                   
                   
                 GTGTTCCTGTTCCCCCCAAAGCCCAAGGACACCCTGATGATCAGCC 
               
               
                   
                   
                 GGACCCCCGAAGTGACCTGCGTGGTGGTGGACGTGTCCCACGAGG 
               
               
                   
                   
                 ACCCTGAAGTGAAGTTCAATTGGTACGTGGACGGCGTGGAGGTGC 
               
               
                   
                   
                 ACAATGCCAAGACCAAGCCCCGGGAGGAACAGTACAACAGCACC 
               
               
                   
                   
                 TACCGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTG 
               
               
                   
                   
                 AACGGCAAAGAGTACAAGTGCAAGGTCTCCAACAAGGCCCTGCCT 
               
               
                   
                   
                 GCCCCCATCGAGAAAACCATCAGCAAGGCCAAGGGCCAGCCCAG 
               
               
                   
                   
                 AGAACCCCAGGTGTGCACCCTGCCCCCCAGCAGAGATGAGCTGAC 
               
               
                   
                   
                 CAAGAACCAGGTGTCCCTGAGCTGTGCCGTCAAGGGCTTCTACCC 
               
               
                   
                   
                 CAGCGATATCGCCGTGGAGTGGGAGAGCAACGGCCAGCCTGAGA 
               
               
                   
                   
                 ACAACTACAAGACCACCCCCCCTGTGCTGGACAGCGACGGCAGCT 
               
               
                   
                   
                 TCTTCCTGGTGTCCAAACTGACCGTGGACAAGAGCCGGTGGCAGC 
               
               
                   
                   
                 AGGGCAACGTGTTCAGCTGCAGCGTGATGCACGAGGCCCTGCACA 
               
               
                   
                   
                 ACCACTACACCCAGAAGTCCCTGAGCCTGAGCCCCGGCAAG