Patent Publication Number: US-2022226473-A1

Title: Nucleic acid nanocarrier drug and preparation method thereof, pharmaceutical composition and application thereof

Description:
TECHNICAL FIELD 
     The present application relates to the field of medicines, and in particular to a nucleic acid nanocarrier drug, a preparation method thereof, a pharmaceutical composition and an application thereof. 
     BACKGROUND 
     In order to alleviate side effects caused by poor targeting of active ingredients of a drug, a drug delivery carrier is generated at the right moment, and a function thereof is to carry the active ingredients of the drug, and deliver the active ingredients into blood or tissue cells so as to treat diseases. There are already a variety of methods to achieve the targeted delivery of the different drugs. It may be achieved with instruments or devices, such as a gene gun, and an electroporator. These methods do not need to use a gene carrier, but the transfection efficiency is generally very low, the operation is complicated, and the damage to a tissue is relatively large. It is also mediated by viral carriers, such as an adenovirus, and a lentivirus. Although the viral carriers have the higher transfection activity in vitro, the immunogenicity and disadvantages thereof which may easily cause mutations bring huge safety risks to in vivo delivery. There are also non-viral carriers, especially a biodegradable polymer material, to achieve the targeted delivery of the drug. A main advantage of the non-viral carriers is that the immunogenicity and many inflammatory reactions brought by the viral carriers may be greatly reduced under a condition of guaranteeing the expected transfection activity. 
     In the above multiple targeted delivery modes, more studies are focused on the field of the non-viral carriers at present, and it is generally a plurality of the following carrier designs: (a) a cationic liposome; and (b) a polycationic gene carrier. At present, the more studies focus on modification of the polycationic gene carrier and the cationic liposome, so that it is suitable for the targeted delivery of the genetic substances. The cationic liposome has the higher transfection activity in vivo and in vitro. However, because normal distribution thereof in vivo is affected by positive charge on a surface, at the same time, the cationic liposome may cause the immunogenicity and the inflammatory responses in animal experiments. The development of the polycationic gene carrier is relatively mature at present, but it is difficult to guarantee that a targeting group is on a surface of a structure in a structural design, and there is an own design contradiction between the toxicity and the transfection activity, at the same time, connection thereof is difficult to achieve non-toxic degradation in vivo. 
     Therefore, how to improve the delivery reliability of an existing small molecular drug is one of the difficulties in solving a limited clinical application of the existing drug. 
     SUMMARY 
     A main purpose of the present application is to provide a nucleic acid nanocarrier drug, a preparation method thereof, a pharmaceutical composition and an application thereof, as to improve the delivery reliability of a drug. 
     In order to achieve the above purpose, according to one aspect of the present application, a nucleic acid nanocarrier drug is provided, and includes a nucleic acid nanoparticle and a drug loaded on the nucleic acid nanoparticle, and the drug includes one or more of tacrine, epirubicin, methotrexate, pirarubicin, daunorubicin, pentafluorouracil, 10-hydroxycamptothecin, aspirin and gemcitabine; the nucleic acid nanoparticle includes a nucleic acid domain, the nucleic acid domain includes a sequence a, a sequence band a sequence c, the sequence a includes a sequence a1 or a sequence obtained by insertion, deletion or substitution of at least one base in the sequence a1, the sequence b comprises a sequence b1 or a sequence obtained by insertion, deletion or substitution of at least one base in the sequence b1, and the sequence c comprises a sequence c1 or a sequence obtained by insertion, deletion or substitution of at least one base in the sequence c1, herein, the sequence a1 is SEQ ID NO:1: 5′-CCAGCGUUCC-3′ or SEQ ID NO:2: 5′-CCAGCGTTCC-3′; the sequence b1 is SEQ ID NO:3: 5′-GGUUCGCCG-3′ or SEQ ID NO:4: 5′-GGTTCGCCG-3′; and the sequence c1 is SEQ ID NO:5: 5′-CGGCCAUAGCGG-3′ or SEQ ID NO:6: 5′-CGGCCATAGCGG-3′. 
     Further, when the sequence a1 is the SEQ ID NO:1, the sequence b1 is the SEQ ID NO:3, and the sequence c1 is the SEQ ID NO:5, at least one sequence of the sequence a, the second b and the sequence c includes a sequence obtained by insertion, deletion or substitution of at least one base in which at least one base is inserted, deleted or substituted within thereof. 
     Further, the insertion, deletion or substitution of at least one base is generated: 
     (1) on 1, 2, 4 or 5-th base starting from a 5′-end of the sequence shown in the SEQ ID NO:1 or the SEQ ID NO:2; and/or 
     (2) between 8-th and 10-th bases starting from the 5′-end of the sequence shown in the SEQ ID NO:1 or the SEQ ID NO:2; and/or 
     (3) between 1-th and 3-th base&#39;s starting from a 5′-end of the sequence shown in the SEQ ID NO:3 or the SEQ ID NO:4; and/or 
     (4) between 6-th and 9-th bases starting from the 5′-end of the sequence shown in the SEQ ID NO:3 or the SEQ ID NO:4; and/or 
     (5) between 1-th and 4-th bases starting from a 5′-end of the sequence shown in the SEQ ID NO:5 or the SEQ ID NO:6; and/or 
     (6) between 9-th and 12-th bases starting from the 5′-end of the sequence shown in the SEQ ID NO:5 or the SEQ ID NO:6. 
     Further, the sequence a, the sequence b and the sequence c are self-assembled into a structure shown in Formula (1) 
     
       
         
           
               
               
            
               
                   
                 Formula (1) 
               
               
                   
                 a 5′WWNWWNNNWW3′ 
               
               
                   
                   3′ CC CC N′N′CC5′ b 
               
               
                   
                          N 
               
               
                   
                          N N′ 
               
               
                   
                          N 
               
               
                   
                          N 
               
               
                   
                          W C 
               
               
                   
                          W C 
               
               
                   
                          W C 
               
               
                   
                          W C 
               
               
                   
                          5′ 3′ 
               
               
                   
                          c. 
               
            
           
         
       
     
     Herein, W-C represents a Watson-Crick pairing, N and N′ represent a non-Watson-Crick pairing, the W-C in any one position is independently selected from C-G or G-C; in the sequence a, the first N from the 5′-end is A, the second N is G, the third N is U or T, and the fourth N is any one of U, T, A, C or G; in the sequence b, the first N′ from the 5′-end is any one of U, T, A, C or G, the second N′ is U or T, and the third N′ is C; and in the sequence c, a sequence NNNN along a direction from the 5′-end to the 3-end is CAUA or CATA. 
     Further, the sequence a, the sequence b and the sequence c are any one of the following groups: (1) sequence a: 5′-GGAGCGUUGG-3′, sequence b: 5′-CCUUCGCCG-3′, sequence c: 5′-CGGCCAUAGCCC-3′; (2) sequence a: 5′-GCAGCGUUCG-3′, sequence b: 5′-CGUUCGCCG-3′, sequence c: 5′-CGGCCAUAGCGC-3′; (3) sequence a: 5′-CGAGCGUUGC-3′, sequence b: 5′-GCUUCGCCG-3′, sequence c: 5′-CGGCCAUAGCCG-3′: (4) sequence a: 5′-GGAGCGUUGG-3′, sequence b: 5′-CCUUCGGGG-3′, sequence c: 5′-CCCCCAUAGCCC-3′; (5) sequence a: 5′-GCAGCGUUCG-3′, sequence b: 5′-CGUUCGGCG-3′, sequence c: 5′-CGCCCAUAGCGC-3′; (6) sequence a: 5′-GCAGCGUUCG-3, sequence b: 5′-CGUUCGGCC-3′, sequence c: 5′-GGCCCAUAGCGC-3′; (7) sequence a: 5′-CGAGCGUUGC-3, sequence b: 5-GCUUCGGCG-3′, sequence c: 5′-CGCCCAUAGCCG-3′; (8) sequence a: 5-GGAGCGTTGG-3′, sequence b: 5′-CCTTCGCCG-3′, sequence c: 6-CGGCCATAGCCC-3′; (9) sequence a: 5′-GCAGCGTTCG-3′, sequence b: 5′-CGTTCGCCG-3, sequence c: 6-CGGCCATAGCGC-3; (10) sequence a: 5′-CGAGCGTTGC-′, sequence b: 5′-GCTTCGCCG-3, sequence c: 5′-CGGCCATAGCCG-3′; (11) sequence a: 5′-GGAGCGTTGG-3′, sequence b: 5-CCTTCGGGG-3, sequence c: 5′-CCCCCATAGCCC-3′; (12) sequence a: 5′-GCAGCGTTCG-3′, sequence b: 5′-CGTTCGGCG-3, sequence c: 5′-CGCCCATAGCGC-3′; (13) sequence a: 5′-GCAGCGTTCG-3′, sequence b: 5′-CGTTCGGCC-3, sequence c: 5′-GGCCCATAGCGC-3′: and (14) sequence a: 5′-CGAGCGTTGC-3′, sequence b: 5′-GCTTCGGCG-3′, sequence c: 5′-CGCCCATAGCCG-3. 
     Further, the nucleic acid domain further includes a first extension fragment, the first extension fragment is an extension fragment of the Watson-Crick pairing, and the first extension fragment is positioned at the 5′-end and/or the 3′-end of any one sequence of the sequence a, the sequence b or the sequence c; preferably, the first extension fragment is selected from any one of the following groups: (1): a-strand 5′-end: 5′-CCCA-3′, c-strand 3′-end: S-UGGG-3′; (2): a-strand 3′-end: 5′-GGG-3′, b-strand 5′-end: 5′-CCC-3′; (3): b-strand 3′-end: 5′-CCA-3′, c-strand 5-end: 5′-UGG-3′; (4): a-strand 5′-end: 5′-CCCG-3′, c-strand 3′-end: 5′-CGGG-3; (5): a-strand 5′-end: 5′-CCCC-3′, c-strand 3′-end: 5′-GGGG-3′; (6): b-strand 3′-end: 5′-CCC-3′, c-strand 5′-end: 5-GGG-3′; (7): b-strand 3′-end: 5′-CCG-3′, c-strand 5′-end: 5′-CGG-3′; (8): a-strand 5′-end: 5′-CCCA-3′, c-strand 3′-end: 5′-TGGG-3′; and (9): b-strand 3′-end: 5′-CCA-3′, c-strand 5′-end: 5′-TGG-3′. 
     Further, the nucleic acid domain further includes a second extension fragment, the second extension fragment is positioned at the 5′-end and/or the 3-end of any one sequence of the sequence a, the sequence b, or the sequence c, and the second extension fragment is an extension fragment of the Watson-Crick pairing; preferably, the second extension fragment is an extension sequence of a CG base pair; and more preferably, the second extension fragment is an extension sequence of 1-10 CG base pairs. 
     Further, the nucleic acid domain further includes at least one group of the following second extension fragments: first group: a-strand 5′-end: 5′-CGCGCG-3′, c-strand 3′-end: 5′-CGCGCG-3′; second group: a-strand 3′-end: 5′-CGCCGC-3′, b-strand 5′-end: 5′-GCGGCG-3′; and third group: b-strand 3′-end: 5-GGCGGC-3′, c-strand 5′-end: 5′-GCCGCC-3′. 
     Further, the second extension fragment is an extension sequence containing both CG base pair and AT/AU base pair, and preferably the second extension fragment is an extension sequence of 2-50 base pairs. 
     Further, the second extension fragment is an extension sequence in which sequences of 2-8 continuous CG base pairs and sequences of 2-8 continuous AT/AU base pairs are alternately arranged; or the second extension fragment is an extension sequence in which a sequence of 1 CG base pair and a sequence of 1 AT/AU base pair are alternately arranged. 
     Further, a base, a ribose and a phosphate in the sequence a, the sequence b and the sequence c have at least one modifiable site, and any one of the modifiable sites is modified by any one of the following modification adapters: —F, a methyl, an amino, a disulfide, a carbonyl, a carboxyl, a sulfhydryl and a formyl; and preferably, the base C or U in the sequence a, the sequence b and the sequence c has 2′-F modification. 
     Further, the drug is loaded on the nucleic acid nanoparticle in modes of physical linkage and/or covalent linkage, and a molar ratio between the drug and the nucleic acid nanoparticle is 2-300:1, preferably 10-50:1, and more preferably 15-25:1. 
     Further, the nucleic acid nanoparticle further include a bioactive substance, the bioactive substance is linked with the nucleic acid domain, and the bioactive substance is one or more of a target head, a fluorescein, an Interfering nucleic acid siRNA, a miRNA, a ribozyme, a riboswitch, an aptamer, a RNA antibody, a protein, a polypeptide, a flavonoid, a glucose, a natural salicylic acid, a monoclonal antibody, a vitamin, a phenol, a lecithin, and a small molecular drug, the small molecular drug is a small molecular drug except the tacrine, the epirubicin, the methotrexate, the pirarubicin, the daunorubicin, the pentafluorouracil, the 10-hydroxycamptothecin, the aspirin and the gemcitabine. 
     Further, a relative molecular weight of the nucleic acid domain is marked as N 1 , and a total relative molecular weight of the drug and the bioactive substance is marked as N 2 , N 1 /N 2 ≥1:1. 
     Further, the bioactive substance is one or more of the target head, the fluorescein and the miRNA, herein the target head is positioned on any one sequence of the sequences a, b and c, preferably the 5′-end or the 3′-end of any one sequence of the sequences a, b and c, or inserted between GC bonds of the nucleic acid domain, the miRNA is an anti-miRNA, the fluorescein is modified at 5′-end or 3-end of the anti-miRNA, and the miRNA is positioned in any one or more positions in the 3′-end of the sequence a, and the 5′-end and the 3′-end of the sequence c, and preferably, the target head is a folic acid or a biotin, the fluorescein is any one or more of FAM, CY5 and CY3, and the anti-miRNA is anti-miR-21. 
     Further, the small molecular drug is a drug containing any one or more of the following groups: an amino group, a hydroxyl group, a carboxyl group, a mercapto group, a benzene ring group and an acetamido group. 
     Further, the protein is one or more of SOD, survivin, hTERT, EGFR and PSMA; the vitamin is L-V C  and/or esterified V C ; and the phenol is a tea polyphenol and/or a grape polyphenol. 
     Further, a particle size of the nucleic acid nanoparticle is 1-100 nm, preferably 5-50 nm; more preferably 10-30 nm; and further preferably 10-15 nm. 
     According to another aspect of the present application, a preparation method for a nucleic acid nanocarrier drug is provided, and the method includes the following steps: the above nucleic acid nanoparticle is provided; and the drug is loaded on the nucleic acid nanoparticle in a physical linkage mode and/or a covalent linkage mode, to obtain the nucleic acid nanocarrier drug. 
     Further, the step of loading the drug in the physical linkage mode includes the drug, the nucleic acid nanoparticle and a first solvent are mixed and stirred, to obtain a premixed system; and the premixed system is precipitated, to obtain the nucleic acid nanocarrier drug; preferably, the first solvent is selected from one or more of DCM, DCC, DMAP, Py, DMSO, PBS and glacial acetic acid; preferably, the step of precipitating the premixed system, to obtain the nucleic acid nanocarrier drug includes the premixed system is precipitated, to obtain a precipitation; and the precipitation is washed to remove impurities, as to obtain the nucleic acid nanocarrier drug; more preferably, the premixed system is mixed with absolute ethyl alcohol, and precipitated at a temperature condition lower than 10 DEG C., to obtain the precipitation, namely the nucleic acid nanocarrier drug; and more preferably, the precipitation is obtained by precipitating at a temperature condition of 0-5 DEG C. More preferably, the precipitation is washed to remove the impurities with 6-12 times of the absolute ethyl alcohol in volume, as to obtain the nucleic acid nanocarrier drug. 
     Further, the step of loading the drug in the covalent linkage mode includes drug solution is prepared; the drug solution reacts with the G-exocyclic amino of the nucleic acid nanoparticle under a mediating effect of the formaldehyde, to obtain a reaction system; and the reaction system is purified, to obtain the nucleic acid nanocarrier drug; preferably, the reaction step includes the drug solution is mixed with paraformaldehyde solution and the nucleic acid nanoparticle, and it is reacted in a dark condition, to obtain the reaction system; herein the concentration of the paraformaldehyde solution is preferably 3.7-4 wt %, and the paraformaldehyde solution is preferably solution formed by mixing paraformaldehyde and a second solvent, and the second solvent is one or more of DCM, DCC, DMAP, Py, DMSO, PBS and glacial acetic acid. 
     Further, the preparation method further includes a step of preparing the nucleic add nanoparticle, the step includes a single strand corresponding to the above nucleic acid domain is self-assembled, to obtain the nucleic acid domain; preferably, after the nucleic acid domain is obtained, the preparation method further includes the above bioactive substance is loaded on the nucleic acid domain in the modes of physical linkage and/or covalent linkage, to obtain the nucleic acid nanoparticle. 
     Further, in a process of loading the bioactive substance in the covalent linkage mode, the loading is performed through solvent covalent linkage, linker covalent linkage or click-linkage; preferably, a third solvent used in the solvent covalent linkage is served as a linkage medium, and the third solvent is selected from one or more of paraformaldehyde, DCM, DCC, DMAP, Py, DMSO, PBS and glacial acetic acid; preferably, the linker is selected from a disulfide bond, a p-phenylazide, bromopropyne or PEG; preferably, the click-linkage is that alkynyl or azide modification is simultaneously performed on a bioactive substance precursor and the nucleic acid domain, and then they are linked through the click-linkage. 
     Further, when the bioactive substance is linked with the nucleic acid domain in the click-linkage mode, a site, for performing the alkynyl or azide modification, of the bioactive substance precursor is selected from a 2′-hydroxyl, a carboxyl or an amino, and a site, for performing the alkynyl or azide modification, of the nucleic acid domain is selected from a G-exocyclic amino, a 2′-hydroxyl, an A-amino or a 2′-hydroxyl. 
     According to a third aspect of the present application, a pharmaceutical composition is further provided, and the pharmaceutical composition includes any one of the above nucleic acid nanocarrier drugs. 
     According to a fourth aspect of the present application, an application of any one of the above nucleic acid nanocarrier drugs in preparing a drug for treating an Alzheimer&#39;s disease, a tumor, an autoimmune disease or a heart disease is further provided. 
     Further, the tumor is one or more of the followings: pancreatic cancer, ovarian cancer, breast cancer, bladder cancer, cervical cancer, liver cancer, biliary tract cancer, nasopharyngeal cancer, testicular cancer, lymphoma, mesothelioma, head and neck cancer, gastric cancer, leukemia, colon cancer, rectal cancer, chorionic epithelioma, malignant hydatidiform mole, skin cancer, lung cancer, ureteral cancer, renal pelvis cancer, chorionic epithelioma, bone tumor, leukemia meningeal spinal cord infiltration, Wilms tumor, soft tissue sarcoma and medullary thyroid carcinoma; the autoimmune disease is refractory psoriasis, systemic lupus erythematosus, mandatory spondylitis or dermatomyositis. 
     Further, the leukemia is acute leukemia, more preferably the acute leukemia is acute lymphocytic leukemia or myeloid leukemia. 
     Further, the lung cancer includes bronchial lung cancer or non-small cell lung cancer. 
     Further, the liver cancer includes primary hepatocellular carcinoma or metastatic liver cancer. 
     According to a fifth aspect of the present application, a method for preventing and/or treating an Alzheimer&#39;s disease, a tumor, an autoimmune disease or a heart disease is further provided, and the method includes any one of the above nucleic acid nanocarrier drugs or pharmaceutical compositions is provided; a corresponding effective dose of the above nucleic acid nanocarrier drug or pharmaceutical composition is administered to a patient. 
     The nucleic acid nanocarrier drug provided by the present application includes the nucleic add nanoparticle and the drug, and the drug is located on the nucleic acid nanoparticle in the modes of the physical linkage and/or the covalent linkage. The nucleic acid nanoparticle, through including the three sequences provided by the present application or the variant sequences thereof, not only may be self-assembled to form the nucleic acid domain, but also may be served as a carrier to link the drug at the arbitrary 5′-end and/or 3-end of the three strands, or enables the drug to be stably inserted between the strands of the nucleic acid domain. The present application is capable of, through loading the drug on the nucleic acid nanoparticle, using internal hydrophobicity, external hydrophilicity and a base stacking effect of the nucleic acid nanoparticle to have a “coating effect” on the drug, and the drug is not dissolved within a certain period of time through the coating effect or the covalent linkage, so the delivery stability is improved. In addition, when the nucleic acid domain is modified by the target head, it may have the better targeting property, and may deliver the drug stably, the reliability is very high; at the same time, it may reduce a chance of the drug in contact with non-target cells or tissues, toxic side effects are reduced. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       Drawings of the description for constituting a part of the present application are used to provide further understanding of the disclosure, exemplary embodiments of the disclosure and descriptions thereof are used to explain the present application, and do not constitute improper limitation to the present application. In the drawings: 
         FIG. 1  shows an electrophoresis detection result of RNA nanoparticles formed by self-assembly in Embodiment 1 of the present application. 
         FIG. 2  shows an electrophoresis detection result of DNA nanoparticles formed by self-assembly in Embodiment 1 of the present application. 
         FIG. 3  shows a 2% agarose gel electrophoresis detection result of 7 groups of short-sequence RNA nanoparticles formed by self-assembly in Embodiment 2 of the present application. 
         FIG. 4  shows a 4% agarose gel electrophoresis detection result of 7 groups of short-sequence RNA nanoparticles formed by self-assembly in Embodiment 2 of the present application. 
         FIG. 5  shows a 2% agarose gel electrophoresis detection result of 7 groups of conventional sequence RNA nanoparticles formed by self-assembly in Embodiment 3 of the present application. 
         FIG. 6  shows a 4% agarose gel electrophoresis detection result of 7 groups of conventional sequence RNA nanoparticles formed by self-assembly in Embodiment 3 of the present application. 
         FIG. 7  shows a 2% agarose gel electrophoresis detection result of 7 groups of conventional sequence DNA nanoparticles formed by self-assembly in Embodiment 4 of the present application. 
         FIG. 8  shows a 4% agarose gel electrophoresis detection result of 7 groups of conventional sequence DNA nanoparticles formed by self-assembly in Embodiment 4 of the present application. 
         FIG. 9  shows a transmission electron microscope picture of conventional sequence DNA nanoparticles D-7 formed by self-assembly in Embodiment 4 of the present application. 
         FIG. 10  shows a standard curve of a tacrine absorbance in a loading rate detection process in Embodiment 5 of the present application. 
         FIG. 11  shows a microscopic observation result of binding and internalization of RNAh-Biotin-quasar670 nanoparticles and RNAh-Biotin-quasar670-tacrine nanoparticles with SH-SY5Y cells in Embodiment 6 of the present application. 
         FIG. 12  shows an electrophoresis detection result of the RNAh-Biotin-quasar670-tacrine nanoparticles, after being incubated in serum for different times, under a Coomassie Blue program in Embodiment 7 of the present application. 
         FIG. 13  shows an electrophoresis detection result of the RNAh-Biotin-quasar670-tacrine nanoparticles, after being incubated in serum for different times, under a Stain Free Gel program in Embodiment 7 of the present application. 
         FIG. 14  shows a detection result of the small molecular drug tacrine and the RNAh-Biotin-quasar670-tacrine nanoparticles for inhibiting proliferation of the SH-SY5Y cells in Embodiment 8 of the present application. 
         FIG. 15  shows a detection result of a fluorescence-targeted carrier Bio-Cy5-RNAh for inhibiting the proliferation of the SH-SY5Y cells in Embodiment 8 of the present application. 
         FIG. 16  shows non-denaturing PAGE gel electrophoresis detection results of 7 groups of extension fragment deformation+core short-sequence RNA self-assembly products in Embodiment 9 of the disclosure. 
         FIG. 17  shows a solubility curve of RNA nanoparticles R-15 in Embodiment 9 of the disclosure. 
         FIG. 18  shows a solubility curve of RNA nanoparticles R-16 in Embodiment 9 of the disclosure. 
         FIG. 19  shows a solubility curve of RNA nanoparticles R-17 in Embodiment 9 of the disclosure. 
         FIG. 20  shows a solubility curve of RNA nanoparticles R-18 in Embodiment 9 of the disclosure. 
         FIG. 21  shows a solubility curve of RNA nanoparticles R-19 in Embodiment 9 of the disclosure. 
         FIG. 22  shows a solubility curve of RNA nanoparticles R-20 in Embodiment 9 of the disclosure. 
         FIG. 23  shows a solubility curve of RNA nanoparticles R-21 in Embodiment 9 of the disclosure. 
         FIG. 24  shows non-denaturing PAGE gel electrophoresis detection results of 7 groups of extension fragment deformation+core short-sequence DNA self-assembly products in Embodiment 10 of the disclosure. 
         FIG. 25  shows a solubility curve of DNA nanoparticles D-8 in Embodiment 10 of the disclosure. 
         FIG. 26  shows a solubility curve of DNA nanoparticles D-9 in Embodiment 10 of the disclosure. 
         FIG. 27  shows a solubility curve of DNA nanoparticles D-10 in Embodiment 10 of the disclosure. 
         FIG. 28  shows a solubility curve of DNA nanoparticles D-11 in Embodiment 10 of the disclosure. 
         FIG. 29  shows a solubility curve of DNA nanoparticles D-12 in Embodiment 10 of the disclosure. 
         FIG. 30  shows a solubility curve of DNA nanoparticles D-13 in Embodiment 10 of the disclosure. 
         FIG. 31  shows a solubility curve of DNA nanoparticles D-14 in Embodiment 10 of the disclosure. 
         FIG. 32  shows an electrophoresis detection result of RNA nanoparticles R-15 after being incubated in serum for different times in Embodiment 11 of the disclosure. 
         FIG. 33  shows an electrophoresis detection result of RNA nanoparticles R-16 after being incubated in serum for different times in Embodiment 11 of the disclosure. 
         FIG. 34  shows an electrophoresis detection result of RNA nanoparticles R-17 after being incubated in serum for different times in Embodiment 11 of the disclosure. 
         FIG. 35  shows an electrophoresis detection result of RNA nanoparticles R-18 after being incubated in serum for different times in Embodiment 11 of the disclosure. 
         FIG. 36  shows an electrophoresis detection result of RNA nanoparticles R-19 after being incubated in serum for different times in Embodiment 11 of the disclosure. 
         FIG. 37  shows an electrophoresis detection result of RNA nanoparticles R-20 after being incubated in serum for different times in Embodiment 11 of the disclosure. 
         FIG. 38  shows an electrophoresis detection result of RNA nanoparticles R-21 after being incubated in serum for different times in Embodiment 11 of the disclosure. 
         FIG. 39  shows an electrophoresis detection result of DNA nanoparticles D-8 after being incubated in serum for different times in Embodiment 12 of the disclosure. 
         FIG. 40  shows an electrophoresis detection result of DNA nanoparticles D-9 after being incubated in serum for different times in Embodiment 12 of the disclosure. 
         FIG. 41  shows an electrophoresis detection result of DNA nanoparticles D-10 after being incubated in serum for different times in Embodiment 12 of the disclosure. 
         FIG. 42  shows an electrophoresis detection result of DNA nanoparticles D-11 after being incubated in serum for different times in Embodiment 12 of the disclosure. 
         FIG. 43  shows an electrophoresis detection result of DNA nanoparticles D-12 after being incubated in serum for different times in Embodiment 12 of the disclosure. 
         FIG. 44  shows an electrophoresis detection result of DNA nanoparticles D-13 after being incubated in serum for different times in Embodiment 12 of the disclosure. 
         FIG. 45  shows an electrophoresis detection result of DNA nanoparticles D-14 after being incubated in serum for different times in Embodiment 12 of the disclosure. 
         FIG. 46 a   ,  FIG. 46 b   ,  FIG. 46 c   ,  FIG. 46 d   ,  FIG. 46 e   ,  FIG. 46 f   ,  FIG. 46 g   , and  FIG. 46 h    respectively show cell survival rate curves corresponding to DMSO and original drug doxorubicin, D-8 and D-8-doxorubicin, D-9 and D-9-doxorubicin, D-10 and D-10-doxorubicin, D-11 and D-11-doxorubicin, D-12 and D-12-doxorubicin, D-13 and D-13-doxorubicin, and D-14 and D-14-doxorubicin in Embodiment 15 of the disclosure. 
         FIG. 47  shows a standard curve of a daunorubicin absorbance used in a loading rate detection process of Embodiment 16. 
         FIG. 48 a    and  FIG. 48 b   ,  FIG. 49 ,  FIG. 50   sa  and  FIG. 50 b   ,  FIG. 51 ,  FIG. 52 ,  FIG. 53 ,  FIG. 54 a    and  FIG. 54 b   , and  FIG. 55  successively show standard curves of absorbance of epirubicin, methotrexate, pirarubicin, daunorubicin, pentafluorouracil, 10-hydroxycamptothecin, aspirin and gemcitabine in the loading rate detection process in Embodiment 17 of the present application. 
         FIG. 56  to  FIG. 63  respectively show binding and internalization ability of the nucleic acid nanoparticles loaded with the epirubicin, the methotrexate, the pirarubicin, the daunorubicin, the pentafluorouracil, the 10-hydroxycamptothecin, the aspirin and the gemcitabine to cells. 
         FIG. 64  to  FIG. 81  respectively show stability of the nucleic acid nanoparticles loaded with the epirubicin ( FIG. 64  to  FIG. 66 ), the methotrexate ( FIG. 67  to  FIG. 68 ), the pirarubicin ( FIGS. 69, 70 and 71 ), the daunorubicin ( FIGS. 72 and 73 ), the pentafluorouracil ( FIGS. 74 and 75 ), the 10-hydroxycamptothecin ( FIGS. 76 and 77 ), the aspirin ( FIGS. 78 and 79 ) and the gemcitabine ( FIGS. 80 and 81 ) in serum. 
         FIG. 82  to  FIG. 101  respectively show toxicity of the nucleic acid nanoparticles loaded with the epirubicin ( FIG. 82 ,  FIG. 83 ,  FIG. 84 a  to 84 d    and  FIG. 85 a  to 85 d   ), the methotrexate ( FIGS. 86 and 87 ), the pirarubicin ( FIGS. 88, 89, and 91   a  to  91   d ), the daunorubicin ( FIGS. 92 and 93 ), the pentafluorouracil ( FIGS. 94 and 95 ), the 10-hydroxycamptothecin ( FIGS. 96 and 97 ), the aspirin ( FIGS. 98 and 99 ) and the gemcitabine ( FIGS. 100 and 101 ) to the cells. 
     
    
    
     DETAILED DESCRIPTION OF THE EMBODIMENTS 
     It is to be noted that embodiments in the present application and features in the embodiments may be combined with each other under a condition without conflicting. The present application is described in detail below with reference to the embodiments. 
     Term Explanation 
     Blank carrier: refers to a blank nucleic acid nanoparticle carrier without containing any bioactive substances, such as an RNAh or a DNAh. 
     Targeting carrier: refers to a nucleic acid nanoparticle carrier which contains a target head but does not contain a fluorescent substance, such as a Biotin-RNAh or a Biotin-DNAh. 
     Fluorescent carrier: refers to a nucleic acid nanoparticle carrier which contains the fluorescent substance but does not contain the target head, such as a Cy5-RNAh or a Cy5-DNAh. 
     Targeted fluorescent carrier: refers to a nucleic acid nanoparticle carrier containing the target head and the fluorescent substance, such as a Biotin-Cy5-RNAh or a Biotin-Cy5-DNAh. 
     Targeted drug: refers to a nucleic acid nanoparticle carrier containing the target head, the fluorescent substance and a chemical drug, such as a tacrine-Biotin-Cy5-RNAh or a tacrine-Biotin-Cy5-DNAh. 
     It is to be noted that there is no special format for a naming rule of each carrier or bioactive substance in the present application, and front and rear positions thereof in the expression do not mean that it is at a 5′end or a 3′end of the RNAh or the DNAh, but only mean that the bioactive substance is contained. 
     As mentioned in the background, although there are a variety of drug carriers for improving drug delivery efficiency in the prior art, it is still difficult to solve the problem of the limited clinical applications of the drugs. In order to improve this situation, the inventor of the present application researches on all existing materials which may be used as the drug carriers, and deeply investigates and analyzes the various carriers in aspects, such as cell/tissue targeting of the carrier, stability in a delivery process, activity and efficiency of entering target cells, drug release ability after reaching the target cells and toxicity to cells, it is discovered that an emerging nanostructure formed by self-assembly of DNA and/or RNA molecules, such as a DNA in a self-assembly system of DNA dendrimers, is used to have a significant obstruction effect to degradation of a nuclease, and have a very important application value in the fields of gene therapy and biomedicine. 
     Through analyzing the existing reported nanoparticle formed by the self-assembly of the DNA and RNA, it is discovered that, compared with the relatively rigid DNA nanoparticle, the RNA nanoparticle has larger flexibility and stronger tension because there are a large number of stem-loop structures within or between molecules, so it has more advantages in an aspect as a candidate drug carrier. However, the RNA nanoparticle in a natural state are relatively poor in stability, and the existing improvements based on application aspects of the RNA nanocarriers are mostly focused on improving the stability and reliability thereof. Although research results at present provide the possibility of loading the drug to a certain extent, they are more focused on researching the possibility and effectiveness of loading nucleic acid drugs, especially a siRNA drug or a miRNA drug and the like. There are few reports at present on whether non-nucleic acid drugs are equally effective. In addition, the existing self-assembled nanoparticle, especially the self-assembled nanoparticle used as carriers, are self-assembled by using a RNA strand, and a very few is self-assembled in a mode of a RNA strand and DNA strand combination, but the self-assembly is achieved without using a pure DNA strand. 
     In order to provide a new RNA nanoparticle carrier with good reliability and self-assembly, the existing RNA nanoparticle are compared and improved by the applicant, a series of new RNA nanoparticle are developed, and in view of improving applicability and reducing cost, the self-assembly is further tried to be performed by using the pure DNA strand, it is unexpectedly discovered that after being changed, these DNA single strands may not only self-assemble into the DNA nanoparticle, but also have the same excellent performance as the RNA nanoparticle. In addition, the set-assembly of the DNA nanoparticle also has advantages of low price and easy operation. After being verified by experiments, both the RNA nanoparticle and the DNA nanoparticle improved by the inventor may be loaded with various drugs, and may exist stably in serum; and further verified by the experiments, it may carry the drugs into the cells, and the separate carrier is non-toxic to the cells. However, the drug-carried carrier may have alleviating and treating effects to corresponding diseases. 
     On the basis of the above research result, the applicant provides a technical scheme of the present application. The present application provides a nucleic acid nanocarrier drug, the nucleic acid nanocarrier drug includes nucleic acid nanoparticle and a drug, the drug is loaded on the nucleic acid nanoparticle, and the drug includes one or more of tacrine, epirubicin, methotrexate, pirarubicin, daunorubicin, pentafluorouracil, 10-hydroxycamptothecin, aspirin and gemcitabine; and the nucleic acid nanoparticle includes a nucleic acid domain, the nucleic acid domain includes a sequence a, a sequence b and a sequence c, the sequence a includes a sequence a1 or a sequence in which at least one base is inserted, deleted or substituted in the sequence a1, the sequence b includes a sequence b1 or a sequence in which at least one base is inserted, deleted or substituted in the sequence b1, and the sequence c includes a sequence c1 or a sequence in which at least one base is inserted, deleted or substituted in the sequence c1, herein the sequence a1 is SEQ ID NO:1: 5′-CCAGCGUUCC-3′ or SEQ ID NO:2: 5′-CCAGCGTTCC-3′; the sequence b1 is SEQ ID NO:3: 5′-GGUUCGCCG-3′ or SEQ ID NO:4: 5′-GGTTCGCCG-3′; and the sequence c1 is SEQ ID NO:5: 5′-CGGCCAUAGCGG-3′ or SEQ ID NO:6: 5′-CGGCCATAGCGG-3′. 
     The nucleic acid nanocarrier drug provided by the present application Includes the nucleic acid nanoparticle and the drug, and one or more of the above drugs are loaded on the nucleic acid nanoparticle. The nucleic acid nanoparticle, through including the above three sequences or the variant sequences thereof, not only may be self-assembled to form the nucleic acid domain, but also may be served as a carrier to link the drug at the arbitrary 5′-end and/or 3′-end of the three strands, or enable the drug to be stably inserted between the strands of the nucleic acid domain. 
     The nucleic acid nanocarrier drug provided by the present application Is capable of, through loading the above drug on the nucleic acid nanoparticle, because the nucleic acid nanoparticle are hydrophobic in the interior, hydrophilic in the exterior and have a stacking effect on the base, it is equivalent to a “coating effect” to the drug, and the drug may not be dissolved within a certain period of time through the coating or the covalent linkage, improving the delivery stability. In addition, when the nucleic acid domain is modified by the target head, it may have the better targeting property, and may deliver the drug stably, the reliability is very high; at the same time, it may reduce a chance of the drug in contact with non-target cells or tissues, toxic side effects are reduced. 
     The above self-assembly refers to a technology that basic structural units spontaneously form an ordered structure. In a process of the self-assembly, the basic structural units spontaneously organize or aggregate into a stable structure with a certain regular geometric appearance under an interaction based on a non-covalent bond. The self-assembly process is not a simple superposition of weak interaction forces (herein the “weak interaction forces” refer to a hydrogen bond, a Van der Waals force, an electrostatic force, a hydrophobic action force and the like) between a large number of atoms, ions or molecules, but a tight and orderly whole formed by simultaneously spontaneously parallel connection and aggregation between a plurality of individuals, and is an overall complicated synergistic effect. 
     The production of the self-assembly requires two conditions: self-assembly power and guiding effect. The self-assembly power refers to the synergistic effect of the weak interaction forces between the molecules, and it provides energy for molecular self-assembly. The guiding effect of the self-assembly refers to complementary of the molecules in space, namely the production of the self-assembly needs to meet requirements of molecular rearrangement in size and direction of the space. 
     A DNA nanotechnology is a bottom-up molecular self-assembly mode, a stable structure is spontaneously formed by using a molecular structure as a starting point on the basis of physical and chemical properties of the nucleic acid nanoparticle, and a strict nucleic acid base pairing principle is followed. Multiple DNA fragments are linked together in vitro in a correct sequence, a sub-assembly structure is established through the base complementary pairing principle, and finally a complicated multi-level structure is formed. Unlike the DNA, the structure of the RNA may exceed limitation of double-helix. The RNA may form a series of different base pairs, and at least two hydrogen bonds are formed between the base pairs. The different bases may be divided into two types, including a standard Waston-Crick base pair type and a non-Waston-Crick base pair type, so that the RNA forms a large number and multiple types of cyclic structure modules, and these modules are basic units for forming a folded RNA three-level structure. The RNA nanotechnology may make use of these natural existing 3D modules and predictable interactions thereof, herein, many RNA structures with biological activity may have an atomic-level resolution, such as a ribosome, various ribozymes and a natural RNA aptamer existing in a riboswitch. A superior feature of the RNA nanotechnology is that a structure which is comparable in size and complexity to a natural RNA substance may be designed. A unique assembly property of the RNA in a natural RNA complex may also be utilized. 
     The above nucleic acid nanoparticle of the present application include three sequences shown in sequences SEQ ID NO:1, SEQ ID NO:3 and SEQ ID NO:5 or variant sequences thereof, or include three sequences shown in sequences SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6 or variant sequences thereof, and all of the sequences are subject to an ability to form the nucleic acid nanoparticle through the self-assembly, the specific variant sequence may be obtained on the basis of the sequences of the SEQ ID NO:1, the SEQ ID NO:2, the SEQ ID NO:3, the SEQ ID NO:4, the SEQ ID NO:5 and the SEQ ID NO:6 by rationally selecting a variant site and a variant type thereof, or obtained by extending a suitable fragment. 
     The nanoparticle formed by the self-assembly of the SEQ ID NO:1, the SEQ ID NO:3 and the SEQ ID NO:5 are the RNA nanoparticle, and the nanoparticle formed by the self-assembly of the SEQ ID NO:2, the SEQ ID NO:4 and the SEQ ID NO:6 are the DNA nanoparticle. In a preferred embodiment, when the above nucleic acid nanoparticle are the RNA nanoparticle, and at least one of the sequence a, the sequence b and the sequence c includes the sequence in which at least one base is inserted, deleted or substituted, a specific position and a base type of the variant sequence in the RNA nanoparticle may be modified into the nanoparticle of improving a drug loading amount or improving stability according to the needs under a precondition of achieving the self-assembly. 
     In order to make the prepared nucleic acid nanoparticle have the relatively higher stability, when the base insertion, deletion or substitution is performed on the sequences shown in the above SEQ ID NO:112, SEQ ID NO:314 and SEQ ID NO:5/6, it may be performed on bases in some specific positions of the above sequences. On the one hand, the variant sequence is the same as the original sequence, and may be self-assembled into the nanoparticle, and on the other hand, the variation retains at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%90% or 95% of homology with the original sequence, so that it has the same drug-loading features and similar stability as the nanoparticle formed by the self-assembly of the above sequences, the drug may be loaded and delivered well. 
     In a preferred embodiment, the above base insertion, deletion or substitution is generated: (1) on 1, 2, 4 or 5-th base starting from a 5′-end of the sequence a shown in the SEQ ID NO:1 or 2; and/or (2) between 8-th and 10-th-bases starting from the 5′-end of the sequence a shown in the SEQ ID NO:1 or 2; and/or (3) between 1-th and 3-th bases starting from a 5′-end of the sequence b shown in the SEQ ID NO 3 or 4; and/or (4) between 6-th and 9-th bases starting from the 5′-end of the sequence b shown in the SEQ ID NO:3 or 4; and/or (5) between 1-th and 4-th bases starting from a 5′-end of the sequence c shown in the SEQ ID NO:5 or 6; and/or (B) between 9-th and 12-th bases starting from the 5′-end of the sequence c shown in the SEQ ID NG 5 or 6. 
     In the above preferred embodiment, the defined base position in which the variation happens is a non-classical Watson-Crick paired base position or a bulged unpaired base position in the nanostructure formed by the sequences shown in the SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3. SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6, thus the formation of these bulges or loop structures is not affected, thereby the flexibility and tension of the nanostructure formed by the above sequences are maintained, and it is helpful to maintain the stability thereof as a carrier. 
     In order to further improve the stability of the above nucleic acid nanoparticle, and improve the stability of the drug after drug loading, in a preferred embodiment, the sequence a, the sequence b and the sequence c are self-assembled into a structure shown in Formula (1): 
     
       
         
           
               
               
            
               
                   
                 Formula (1) 
               
               
                   
                 a 5′WWNWWNNNWW3′ 
               
               
                   
                   3′ CC CC N′N′CC5′ b 
               
               
                   
                          N 
               
               
                   
                          N N′ 
               
               
                   
                          N 
               
               
                   
                          N 
               
               
                   
                          W C 
               
               
                   
                          W C 
               
               
                   
                          W C 
               
               
                   
                          W C 
               
               
                   
                          5′ 3′ 
               
               
                   
                          c 
               
            
           
         
       
     
     herein, W-C represents Watson-Crick pairing, N and N′ represent non-Watson-Crick pairing, the A-C in any one position is independently selected from C-G or G-C; in the sequence a, the first N from the 5′-end is A, the second N is G, the third N is U or T, and the fourth N is any one of U, T, A, C or G; in the sequence b, the first N′ from the 5′-end is any one of U, T, A, C or G, the second N′ is U or T, and the third N′ is C; and in the sequence c, a sequence NNNN along a direction from the 5′-end to the 3′-end is CAUA or CATA. 
     In the above preferred embodiment, the sequences a, b and c are self-assembled to form the nucleic acid domain shown in Formula (1), herein, except the non-Watson-Crick paired bases defined by N and N′, the bases in the rest positions all form classical Watson-Crick pairing, and the bases of the above Watson-Crick pairing all choose G-C or C-G base pairs. Because an action force of hydrogen bonds between the G-C or C-G base pairs is greater than an action force of hydrogen bonds between the A-U/T or U/T-A base pairs, the nucleic acid nanostructure is more stable. Rather than the bulges or loop structures formed by the non-Watson-Crick paired bases, the greater tension is brought to the nucleic acid nanocarrier, so that it has the stronger adaptation to a micro-environmental change, thus the stability of the nucleic acid nanoparticle is higher. 
     In the nanoparticle in the above structure of Formula (1), the specific sequence formation of the sequence a, the sequence b and the sequence c is not specialty limited, as long as the above structure may be formed. In view of the self-assembly of the nucleic acid sequence, in order to further improve the efficiency of the self-assembly of the above three sequences into the nanoparticle in the above structure of Formula (1), when the Watson-Crick paired bases are selected, the selection of the bases in the different positions is best to follow the following principles: (1) the sequence a, the sequence b and the sequence c, when a single sequence is selected, it is not complementary-paired by itself to form a two-level structure; and (2) the sequence a, the sequence b and the sequence c, one end of arbitrary two sequences is complementary-paired to form a double-strand, and the other end is not complementary-paired, to form a Y-type or T-type structure. The above principle of the base selection is to most efficiently enable two ends of any one strand to be respectively complementary-paired with two ends of the other two strands, thereby the self-assembly efficiency is improved. Certainly, in addition to the Y-type or T-type structure, it may also be other deformation modes such as a quadrangle rather than a trigeminal shape, as long as it meets the principle that one end of arbitrary two sequences is complementary-paired to form the double-strand, and the other end is not complementary-paired. 
     In the nanoparticle in the above structure of Formula (1), in the non-Watson-Crick paired bases, the fourth N starting from the 5′-end in the sequence a and the first N′ starting from the 5′-end paired in the sequence b may be non-Watson-Crick paired U-U, or may be T. A, C or G improved for following the Watson-Crick pairing principle. A binding force between the strands is relatively improved by the Watson-Crick pairing, the stability is improved, and the nanoparticle are endowed with the larger softness and flexibility by the non-Watson-Crick pairing, in the face of the micro-environmental change, it is also helpful to improve the stability of the nanoparticle. 
     In a preferred embodiment, the sequence a, the sequence b and the sequence c are any one of the following groups: (1) sequence a (SEQ ID NO:7): 5′-GGAGCGUUGG-3′, sequence b (SEQ ID NO:8): 5′-CCUUCGCCG-3, sequence c (SEQ ID NO:9): 5′-CGGCCAUAGCCC-3; (2) sequence a (SEQ ID NO:10): 5-GCAGCGUUCG-3′, sequence b (SEQ ID NO:11): 5′-CGUUCGCCG-3′, sequence c (SEQ ID NO:12): 5-CGGCCAUAGCGC-3′; (3) sequence a (SEQ ID NO:13): 5′-CGAGCGUUGC-3, sequence b (SEQ ID NO:14): 5′-GCUUCGCCG-3, sequence c (SEQ ID NO:15): 5′-CGGCCAUAGCCG-3; (4) sequence a (SEQ ID NO:16): 5-GGAGCGUUGG-3, sequence b (SEQ ID NO:17): 5-CCUUCGGGG-1, sequence c (SEQ ID NO:18): 5′-CCCCCAUAGCCC-3′; (5) sequence a (SEQ ID NO:19): 5′-GCAGCGUUCG-3′, sequence b (SEQ ID NO:20): 5′-CGUUCGGCG-3′, sequence c (SEQ ID NO:21): 5′-CGCCCAUAGCGC-3′; (6) sequence a (SEQ ID NO:22): 5′-GCAGCGUUCG-3′, sequence b (SEQ ID NO:23): 5′-CGUUCGGCC-3, sequence c (SEQ ID NO:24): 5′-GGCCCAUAGCGC-3′; (7) sequence a (SEQ ID NO:25): 5′-CGAGCGUUGC-3′, sequence b (SEQ ID NO:26): 5′-GCUUCGGCG-3′, sequence c (SEQ ID NO:27): 5′-CGCCCAUAGCCG-3; (8) sequence a (SEQ ID NO:28): 5′-GGAGCGTTGG-3′, sequence b (SEQ ID NO:29): 5′-CCTTCGCCG-3′, sequence c (SEQ ID NO:30): 5′-CGGCCATAGCCC-3; (9) sequence a (SEQ ID NO:31): 5′-GCAGCGTTCG-3′, sequence b (SEQ ID NO:32): 5-CGTTCGCCG-3′, sequence c (SEQ ID NO:33): 5′-CGGCCATAGCGC-3′; (10) sequence a (SEQ ID NO:34): 6-CGAGCGTTGC-3′, sequence b (SEQ ID NO:35): 5′-GCTTCGCCG-3′, sequence c (SEQ ID NO:38): 5′-CGGCCATAGCCG-3′; (11) sequence a (SEQ ID NO:37): 5-GGAGCGTTGG-3′, sequence b (SEQ ID NO:38): 5′-CCTTCGGGG-7, sequence c (SEQ ID NO:39): 5′-CCCCCATAGCCC-3; (12) sequence a (SEQ ID NO:40): 5′-GCAGCGTTCG-3′, sequence b (SEQ ID NO:41): 5′-CGTTCGGCG-3′, sequence c (SEQ ID NO:42): 5′-CGCCCATAGCGC-3; (13) sequence a (SEQ ID NO:43): 5′-GCAGCGTTCG-3′, sequence b (SEQ ID NO:44): 5′-CGTTCGGCC-3′, sequence c (SEQ ID NO:45): 5-GGCCCATAGCGC-3′; and (14) sequence a (SEQ ID NO:46): 5′-CGAGCGTTGC-3, sequence b (SEQ ID NO:46): 5′-GCTTCGGCG-3′, sequence c (SEQ ID NO:48): 5′-CGCCCATAGCCG-3′. 
     The nucleic acid nanoparticle formed by the self-assembly of fourteen groups of the above sequences not only have the higher stability, but also have the higher self-assembly efficiency. 
     The nucleic acid nanoparticle mentioned above may not only be self-assembled for forming, but also have the ability to carry or load the drugs. According to the different positions of G-C or C-G base pairs in the above nucleic acid nanoparticle and differences of types or natures of the drugs to be loaded, the amounts of the loaded drugs are also different. 
     In order to enable the above nucleic acid domain to load more drugs and other bioactive substances (the introduction of the bioactive substance is described below), in a preferred embodiment, the above nucleic acid domain further includes a first extension fragment, the first extension fragment is an extension fragment of Watson-Crick pairing, and the first extension fragment is located at the 5′-end and/or 3-end of any one of the sequences a, b, and c. A certain matching relation is required between the carrier and the loaded substance. Mile a molecular weight of the carrier is too small and a molecular weight of the loaded substance is too large, in view of mechanics, carrying or delivering capacity of the carrier to the loaded substance is relatively reduced. Therefore, based on the above nucleic acid nanostructure, through adding the first extension fragment at the 5′-end and/or 3′-end of any one sequence of the sequence a, the sequence b and the sequence c, the carrier matched with the size of the loaded substance may be acquired. 
     A specific length of the above first extension fragment may be determined according to the size of the substance to be loaded. In a preferred embodiment, the first extension fragment is selected from any one of the following groups: (1): a-strand 5′-end: 6-CCCA-3′, v-strand 3′-end: 6-UGGG-3; (2): a-strand 3′-end: 5-GGG-3′, b-strand 5′-end: 5′-CCC-3′; (3): b-strand 3′-end: 5′-CCA-3′, c-strand 5′-end: 5′-UGG-3′; (4): a-strand 5′-end: 5′-CCCG-3′, c-strand 3′-end: 5′-CGGG-3′; (5): a-strand 5′-end: 5′-CCCC-3′, c-strand 3′-end: 5′-GGGG-3′; (6): b-strand 3′-end: 6-CCC-3′, c-strand 5′-end: 5′-GGG-3′; (7): b-strand 3′-end: 5′-CCG-3, c-strand 5′-end: 5′-CGG-3′; (8): a-strand 5′-end: 5′-CCCA-3′, c-strand 3′-end: 5′-TGGG-3′; (9): b-strand 3′-end: 5-CCA-3, c-strand 5′-end: 5-TGG-3′; (10): a-strand 5′-end: 5′-GCGGCGAGCGGCGA-3′(SEQ ID NO:162), c-strand 3′-end: 5′-UCGCCGCUCGCCGC-3′(SEQ ID NO:163); (11): a-strand 3′-end: 5′-GGCCGGAGGCCGG-3′(SEQ ID NO:164), b-strand 5′-end: 5′-CCGGCCUCCGGCC-3′(SEQ ID NO:165); (12): b-strand 3′-end: 5′-CCAGCCGCC-3′(SEQ ID NO:166), c-strand 5′-end: 5′-GGCGGCAGG-3(SEQ ID NO:167); (13): a-strand 5′-end: 5′-GCGGCGAGCGGCGA-3′(SEQ ID NO:168), c-strand 3′-end: 5-TCGCCGCTCGCCGC-3′(SEQ ID NO:169); and (14): a-strand 3′-end: 5′-GGCCGGAGGCCGG-3′(SEQ ID NO:170), b-strand 5′-end: 5′-CCGGCCTCCGGCC-3(SEQ ID NO:171). 
     The above first extension fragment not only increases a length of any one or more of the three sequences for forming the nucleic acid nanostructure, but also the first extension fragment formed by the GC base further improves the stability of the formed nanoparticle. In addition, the first extension fragment formed by the above sequences also enables the sequence a, the sequence b and the sequence c to maintain the higher self-assembly activity and efficiency. 
     In view of the size of the formed nucleic acid nanoparticle and the stability thereof when delivered as a drug delivery carrier in vivo, it is necessary not to be filtered out by kidneys before reaching the target cells when the drugs may be delivered. In a preferred embodiment, the nucleic acid domain further includes a second extension fragment, the second extension fragment is positioned at the 5′-end and/or the 3′-end of any one sequence of the sequence a, the sequence b, or the sequence c, and the second extension fragment is an extension fragment of Watson-Crick pairing; more preferably, the second extension fragment is an extension sequence of a CG base pair; and further preferably, the second extension fragment is an extension sequence of 1-10 CG base pairs. The second extension fragment is the extension fragment further added on the basis of the first extension fragment. 
     In a preferred embodiment, the above nucleic acid domain further includes at least one group of the following second extension fragments: first group: a-strand 5′-end: 5′-CGCGCG-3′, c-strand 3′-end: 5′-CGCGCG-3′; second group: a-strand 3′-end: 5′-CGCCGC-3′, b-strand 5′-end: 5′-GCGGCG-3′; and third group: b-strand 3′-end: 5′-GGCGGC-3′, c-strand 5′-end: 5′-GCCGCC-3′. Such a second extension fragment enables the nanoparticle not to have immunogenicity, and there is not a situation of the two-level structure in which each strand is folded and linked by itself. 
     It is to be noted that the above first extension fragment and/or second extension fragment may also be separated by an unpaired base pair. 
     In order to enable the above nucleic acid nanoparticle to load the bioactive substance (the introduction of the bioactive substance is described below) with the larger molecular weight, increase the drug loading amount and maintain the necessary stability, in a preferred embodiment, the second extension fragment is an extension sequence containing both CG base pair and AT/AU base pair, and preferably the second extension fragment is an extension sequence of 2-60 base pairs. Here, “/” in the “AT/AU base” is a relation of or, specifically, the second extension fragment is an extension sequence containing both CG base pair and AT base pair, or the second extension fragment is an extension sequence containing both CG base pair and AU base pair. 
     More specifically, the sequences a, b and c after the above second extension fragment is added may be the following sequences respectively: 
     The sequence a is (SEQ ID NO:49): 
     
       
         
           
               
            
               
                 5′-CGCGCGAAAAAACGCGCGAAAAAACGCGCGCCCACCAGCGMMCCGGGC 
               
               
                   
               
               
                 GCGCGAAAAAACGCGCGAAAAAACGCGCG-3′. 
               
            
           
         
       
     
     The sequence b is (SEQ ID NO:50): 
     
       
         
           
               
            
               
                 5′-CGCGCGMMMMMMCGCGCGMMMMMMCGCGCGCCCGGMMCGCCGCCAGCC 
               
               
                   
               
               
                 GCCMMMMMMGCCGCCMMMMMMGCCGCC-3′. 
               
            
           
         
       
     
     The sequence c is (SEQ ID NO:51): 
     
       
         
           
               
            
               
                 5′-GGCGGCAAAAAAGGCGGCAAAAAAGGCGGCAGGCGGCAMAGCGGMGGG 
               
               
                   
               
               
                 CGCGCGMMMMMMCGCGCGMMMMMMCGCGCG-3′. 
               
            
           
         
       
     
     M in the above sequence a, sequence b and sequence c is U or T, when the M is the T, a synthetic cost of the above sequences is greatly reduced. 
     In practical applications, specific setting positions of the extension sequences of the above CG base pair and AT/AU base pair may be rationally adjusted according to actual needs. In a more preferred embodiment, the second extension fragment is an extension sequence in which sequences of 2-8 continuous CG base pairs and sequences of 2-8 continuous AT/AU base pairs are alternately arranged; or the second extension fragment is an extension sequence in which a sequence of 1 CG base pair and a sequence of 1 AT/AU base pair are alternately arranged. 
     Specifically, positions of the extension fragment CGCGCG and the extension fragment CGCCGC in the sequence a as shown in the above SEQ ID NO:49 are interchanged with a position of the extension fragment AAAAAA, positions of the extension fragment GCGGCG and the extension fragment GGCGGC in the sequence b shown in the above SEQ ID NO:50 are interchanged with a position of the extension fragment TTTTTT, the extension fragment GCCGCC in the sequence c shown in the above SEQ ID NO:51 is interchanged with the extension fragment AAAAAA, and the extension fragment CGCCGC is interchanged with the extension fragment TTTTTT at the same time. The nucleic acid nanoparticle formed by the self-assembly of the above sequences are suitable for loading bioactive substances in indole molecular structures (indole drug molecules are preferably inked with A). 
     In the past few years, three major challenges of the RNA as a widely used construction material includes 1) sensitivity to RNA enzymatic degradation; 2) sensitivity to dissociation after systemic injection; and 3) toxicity and adverse immune response. At present, the three challenges are overcome to a large extent already: 1) 2′-fluoro(2-F) or 2′-O-methyl(2′-OMe) modification of a ribose-OH group may make the RNA chemically stable in serum; 2) some natural existing inking sequence motifs are thermodynamically stable, and may keep the overall RNA nanoparticle to be integral at an ultra-low concentration; and 3) the immunogenicity of the RNA nanoparticle is sequence and shape-dependent, and may be adjusted, so that the RNA nanoparticle stimulate generation of inflammatory cytokines, or the RNA nanoparticle have non-immunogenicity and non-toxicity when administered by repeated intravenous injection of 30 mg/kg. 
     Therefore, in order to further reduce the sensitivity of the above nucleic acid nanoparticle to the RNA enzymatic degradation, and improve the stability in the delivery process, in a preferred embodiment, a base, a ribose and a phosphate in the sequence a, the sequence b and the sequence c have at least one modifiable site, and any one of the modifiable sites is modified by any one of the following modification linkers: —F, a methyl, an amino, a disulfide, a carbonyl, a carboxyl, a sulfhydryl and a formyl; and preferably, the base C or U in the sequence a, the sequence b and the sequence c has 2′-F modification. While the modification inker is the sulfhydryl, it belongs to a thio modification, modification strength is weaker, and a cost is low. 
     The above drug may be loaded in the modes of the physical linkage and/or the covalent linkage. While the drug is linked with the nucleic acid domain by using two modes of the physical insertion and the covalent linkage simultaneously, the physical insertion is usually inserted between the GC base pairs, the number of the preferred insertion sites is based on the different numbers of the GC base pairs on the the nucleic acid domain, and the insertion is performed according to the ratio of 1 to 100:1. While the covalent linkage mode is used for linkage, the above drug usually chemically reacts with a G-exocyclic amino to form the covalent linkage. More preferably, a molar ratio between the drug and the nucleic acid nanoparticle is 2 to 300:1, preferably 2 to 290:1, more preferably 2 to 29:1, further preferably 10 to 50:1, and most preferably 15 to 25:1. 
     In the nucleic acid nanocarrier drug provided in the present application, the nucleic acid nanoparticle are served as a drug delivery carrier. In addition, according to different drug purposes, in a preferred embodiment, the above nucleic acid nanoparticle also include the bioactive substance, and the bioactive substance is linked with the nucleic acid domain. The bioactive substance is one of more of a target head, a fluorescein, an interfering nucleic acid siRNA, an miRNA, a ribozyme, a riboswitch, an aptamer, a RNA antibody, a protein, a peptide, a flavonoid, a glucose, a natural salicylic acid, a monoclonal antibody, a vitamin, a phenol, a lecithin and a small molecular drug, herein the small molecular drug does not include the tacrine, the epirubicin, the methotrexate, the pirarubicin, the daunorubicin, the pentafluorouracil, the 10-hydroxycamptothecin, the aspirin and the gemcitabine. 
     In order to improve loading efficiency and delivering efficiency of the nucleic acid nanoparticle to the loaded bioactive substance, a relative molecular weight of the the nucleic acid domain and a total relative molecular weight of the drug and the bioactive substance should preferably have a certain matching relation. In a preferred embodiment, the relative molecular weight of the nucleic acid domain is marked as N1, and the total relative molecular weight of the drug and the bioactive substance is marked as N2, N1/N2≥1:1. 
     According to the different types of the bioactive substances loaded specifically, performance optimization effects thereof on the nucleic acid nanoparticle of the present application are not the same. For example, when the bioactive substance is a biotin or a folio acid, a function thereof is to make the nucleic acid nanoparticle have a targeting property, for example, specifically targeted to cancer cells. While the bioactive substance is the fluorescein, a function thereof is to make the nucleic acid nanoparticle have a luminous tracing effect. While the bioactive substance is some siRNA, miRNA, protein, peptide, RNA antibody and small molecular drug, according to the different biological functions, the nucleic acid nanocarrier drug may be made into a new product with a specific therapeutic effect, such as a drug with more excellent performance. In addition, according to the different types of the bioactive substances loaded specifically, the DNA nanoparticle and the RNA nanoparticle are specifically preferably used, and may be rationally selected according to the actual needs. For example, when the bioactive substance is the drug, the DNA nanoparticle and the RNA nanoparticle are preferably used for loading, and there is no special requirement for a length of the single strand of the nanoparticle formed by assembly. 
     In a preferred embodiment, the bioactive substance is the target head, the fluorescein and the miRNA, herein, the target head is positioned on any one sequence of the sequences a, b and c, preferably the 5′-end or the 3′-end of any one sequence of the sequences a, b and c, or inserted between GC bonds of the the nucleic acid domain, the miRNA is an anti-miRNA, the fluorescein is modified at 5′-end or 3-end of the anti-miRNA, and the miRNA is positioned in any one or more positions in the 3′-end of the sequence a, and the 5′-end and the 3′-end of the sequence c; and preferably, the target head is the folic acid or the biotin, the fluorescein is any one or more of FAM, CY5 and CY3, and the anti-miRNA is anti-miR-21. 
     The above target head may be inked on any one sequence of the sequences a, b and c in a mode of linker covalent linkage, the available linker is selected from a disulfide bond, a p-phenylazide, bromopropyne or PEG. Here, the “on any one sequence” refers to on a base in any one position of any one sequence of the sequences a, b and c, and it is more convenient to be linked at the 5′-end or 3′-end, the application is wider. Folic acid modification may be physical insertion mode linkage or physical insertion+covalent linkage. 
     The above fluorescein may be a commonly used fluorescein, and preferably any one of more of FAM, CY5 and CY3. 
     The above miRNA may be a miRNA with a cancer suppression effect, or an anti-miRNA which may suppress a corresponding disease, and it may be rationally selected in practical applications according to medical needs. The above anti-miRNA may be synthesized at any one or more positions of the 3-end of the above sequence a, the 5′-end and 3′-end of the sequence c. While the anti-miRNA is synthesized in the above three positions, the anti-miRNA has a relatively stronger suppression effect on the corresponding miRNA. 
     It is anti-miR-21 preferably, the MiR-21 participates in initiation and progression of multiple types of the cancers, and is a main oncogene for invasion and metastasis. The anti-miR-21 may effectively regulate a wide range of target genes at the same time, and is beneficial to solve a problem of cancer heterogeneity. Therefore, in the above preferred nucleic acid nanoparticle, the target head, such as the folic acid or the biotin, may be specifically targeted to the cancer cells, and after being inked and internalized with the cancer cells, the anti-miR-21 is complemented with a miR-21 base in very high affinity and specificity, thereby the expression of the oncogenic miR-21 is effectively reduced. Therefore, according to the actual needs, the above anti-miR-21 may be synthesized at any one or more positions of the 3′-end of the above sequence a, the 5′-end and the 3′-end of the sequence c. While the anti-miR-21 is synthesized in the above three positions, the anti-miR-21 has a relatively stronger suppression effect on the miR-21. 
     While the above bioactive substance capable of loading is other small molecular drugs except the tacrine, the epirubicin, the methotrexate, the pirarubicin, the daunorubicin, the pentafluorouracil, the 10-hydroxycamptothecin, the aspirin and the gemcitabine, the nucleic acid nanocarrier drug, according to types of diseases which may be treated by the different drugs, includes but not limited to drugs for treating liver cancer, gastric cancer, lung cancer, breast cancer, head and neck cancer, uterine cancer, ovarian cancer, melanoma, leukemia. Alzheimer&#39;s disease, ankylosing spondylitis, malignant lymphoma, bronchial cancer, rheumatoid arthritis, HBV hepatitis B, multiple myeloma, pancreatic cancer, non-small cell lung cancer, prostate cancer, nasopharyngeal cancer, esophageal cancer, oral cancer, lupus erythematosus; and preferably, the head and neck cancer is brain cancer, neuroblastoma or glioblastoma. 
     While the above bioactive substance capable of loading is other small molecular drugs except the above tacrine and the like, according to a difference of molecular structures of the drugs or a difference of characteristic groups which it has, the drug includes but not limited to a drug containing any one or more of the following groups: an amino group, a hydroxyl group, a carboxyl group, a mercapto group, a benzene ring group and an acetamido group. 
     In a preferred embodiment, the above protein is one or more of antibodies or aptamers of superoxide dismutase (SOD), survivin, human telomerase reverse transcriptase (hTERT), epidermal growth factor receptor (EGFR) and prostate-specific membrane antigen (PSMA); the above vitamin is L-V C  and/or esterified V C ; and the above phenol is a tea polyphenol and/or a grape polyphenol. 
     In a preferred embodiment, a particle size of the nucleic acid nanoparticle is 1-100 nm, preferably 5-50 nm; more preferably 10-30 nm; and further preferably 10-15 nm. The size is appropriate within this range, it may not only enter a cell membrane through cell phagocytosis mediated by a cell surface receptor, but also avoid non-specific cell penetration so as to be filtered and removed by the kidneys. Therefore, the favorable particle size is helpful to improve pharmacokinetics, pharmacodynamics, biological distribution and toxicological distribution. 
     According to a second aspect of the present application, a preparation method for the above nucleic acid nanocarrier drug is further provided, and the preparation method includes the following steps: any one of the above nucleic acid nanoparticle is provided; a drug is loaded on the nucleic acid nanoparticle in modes of physical linkage and/or covalent linkage, to obtain the nucleic acid nanocarrier drug. 
     While the physical linkage mode is used, the drug may be usually formed and inserted between GC base pairs in a physical insertion form. While the covalent linkage mode is used for linkage, the drug usually chemically reacts with a G-exocyclic amino to form the covalent linkage. The nucleic acid nanocarrier drug prepared by using the above method may have the better targeting property after it is modified by the target head, the drug may be stably delivered, and the reliability is very high. 
     In a preferred embodiment, the step of loading the drug in the physical linkage mode includes enabling the drug, the nucleic acid nanoparticle and a first solvent to be mixed and stirring, to obtain a premixed system; and precipitating the premixed system, to obtain the nucleic acid nanocarrier drug. Specific dosages of the drug and the nucleic acid nanoparticle may be adjusted according to a change of the loading amount, this may be understood by those skilled in the art, and it is not repeatedly described here. 
     In order to improve the efficiency and the stability of the physical linkage, an amount of the drug added per liter of the first solvent is preferably 0.1 to 1 g. Preferably, the first solvent is selected from one or more of DCM, DCC, DMAP, Py, DMSO, PBS and glacial acetic acid. Preferably, the step of precipitating the premixed system, to obtain the nucleic acid nanocarrier drug includes precipitating the premixed system, to obtain a precipitation; and washing the precipitation to remove impurities, to obtain the nucleic acid nanocarrier drug. More preferably, the premixed system is mixed with absolute ethyl alcohol, and precipitated at a temperature condition lower than 10 DEG C., to obtain the precipitation, further preferably, the precipitation is obtained by precipitating at a temperature condition of 0-5 DEG C. More preferably, the precipitation is washed to remove the impurities with 6-12 times of the absolute ethyl alcohol in volume, as to obtain the nucleic acid nanocarrier drug. 
     In a preferred embodiment, the step of loading the drug in the covalent linkage mode includes drug solution is prepared; the drug solution reacts with the G-exocyclic amino of the nucleic acid nanoparticle under a mediating effect of the formaldehyde, to obtain a reaction system; and the reaction system is purified, to obtain the nucleic acid nanocarrier drug. 
     Through a formaldehyde-mediated form, the following reaction may occur: 
     
       
         
         
             
             
         
       
     
     Preferably, the above reaction step includes the drug solution is mixed with paraformaldehyde solution and the nucleic acid nanoparticle, and it is reacted in a dark condition, to obtain the reaction system; herein the concentration of the paraformaldehyde solution is preferably 3.7-4 wt %, and the paraformaldehyde solution is preferably solution formed by mixing paraformaldehyde and a second solvent, and the second solvent is one or more of DCM, DCC, DMAP, Py, DMSO, PBS and glacial acetic acid. 
     In the above preparation method, the nucleic acid nanoparticle may be prepared in a mode of self-assembly, for example: (1) enabling the RNA or DNA single strands a, b and c to be simultaneously mixed and dissolved in DEPC water or TMS buffer solution; (2) heating mixed solution to 80/95 DEG C. (herein a RNA assembly temperature is 80 DEG C., and a DNA assembly temperature is 95 DEG C.), after keeping for 5 min, slowly cooling to a room temperature at a rate of 2 DEG C./min; (3) enabling a product to be loaded on 8% (m/v) of non-denaturing PAGE gel and placed in TBM buffer solution under a condition of 4 DEG C., purifying a complex by 100 v of electrophoresis; and (4) cutting a target band and eluting in RNA/DNA elution buffer solution at 37 DEG C., after that, precipitating overnight in ethanol, volatilizing under reduced pressure and low temperature, to obtain a self-assembly product, namely the nucleic acid domain, and then acquiring the nucleic acid nanoparticle. 
     In order to make the above nucleic acid nanocarrier drug have other functions, in a preferred embodiment, after the nucleic acid domain is obtained, the preparation method further includes enabling the bioactive substance as mentioned above to be loaded on the the nucleic acid domain in the modes of physical linkage and/or covalent linkage, to obtain the nucleic acid nanoparticle. The loading mode of the bioactive substance may also be the physical linkage and/or the covalent linkage. The covalent linkage mode includes but not limited to the solvent covalent linkage, the linker covalent linkage or the click-linkage; preferably, a third solvent used in the solvent covalent linkage is served as a linkage medium, and the third solvent is selected from one or more of paraformaldehyde, DCM, DCC, DMAP, Py, DMSO, PBS and glacial acetic acid; preferably, the linker is selected from a disulfide bond, a p-phenylazide, bromopropyne or a PEG; and preferably, the click-linkage is that alkynyl or azide modification is simultaneously performed on a bioactive substance precursor and the the nucleic acid domain, and then they are linked through the click-linkage. 
     It is to be noted that the above classification does not mean that there is only one linkage mode to link a certain bioactive substance with the nucleic acid nanocarrier. However, some bioactive substances may be linked with the nucleic acid nanocarrier in the physical insertion mode, or may be linked with the nucleic acid nanocarrier in the physical insertion and covalent linkage modes, or may be inked by using the click-linkage mode at the same time. However, for a certain specific bioactive substance, there may be only one linkage mode, or there may be multiple linkage modes, but it may be that the efficiency of certain linkage has an advantageous practical value. 
     In the above linkage modes, when the different drugs are linked with the the nucleic acid domain in the physical insertion mode, insertion linkage sites and numbers are also different slightly. For example, when anthracycline and acridine drugs are inserted, they are usually inserted between the GC base pairs, the preferred number of the insertion sites is based on the different numbers of the GC base pairs in the the nucleic acid domain, and the insertion is performed in a ratio of 1 to 100:1. While a naphthylamide drug is inserted, it is usually inserted between the AA base pairs, the preferred number of the insertion sites is based on the different numbers of the AA base pairs in the the nucleic acid domain, and pyridocarbazoles are inserted according to the different numbers of the AA base pairs in a ratio of 1 to 200:1. 
     Specifically, according to the different types of the bioactive substances, the lengths of the sequences a, b and c for forming the the nucleic acid domain in the nucleic acid nanoparticle and the number of GC complementary base pairs thereof, the physical insertion may be performed by rationally selecting a molar ratio of the bioactive substance and the the nucleic acid domain. 
     In a preferred embodiment, when the bioactive substance is inked with the nucleic acid domain in the physical insertion mode and the covalent linkage mode, a molar ratio of the bioactive substance linked in the physical insertion mode and the drug linked in the covalent linkage mode is 1 to 200:1. The linkage mode is suitable for the anthracycline and acridine drugs. A proportion of the drugs linked in the above different linkage modes is not limited to the above range, as long as it may meet a requirement of high-efficient loading, there is a non-toxic effect on the cells, and the effective release of the drug is achieved after reaching a target. 
     While a bioactive substance precursor and the the nucleic acid domain are simultaneously modified by an alkynyl or an azide, and linked in the click-linkage mode, the different click-linkages are selected according to changes of the different structures of the drugs. In addition, along with the different structures of the bioactive substances, the linkage positions may also be changed correspondingly, this may be understood by those skilled in the art. 
     In a preferred embodiment, when the bioactive substance is linked with the the nucleic acid domain in the click-linkage mode, a site, for performing the alkynyl or azide modification, of the bioactive substance precursor is selected from a hydroxyl, a carboxyl, a mercapto or an amino, and a site, for performing the alkynyl or azide modification, of the the nucleic acid domain is selected from the amino, an imino or the hydroxyl. 
     It is to be noted that when the above nucleic acid domain is inked with the drug, the nucleic acid domain is water-soluble, and most of the drugs have poor water solubility. After it is linked with the nucleic acid domain, the water solubility is improved. When the above drugs are anthracyclines, these drugs are covalent-linked with the nucleic acid domain through a —NH bond (under a suitable pH value condition, the activity of the —NH group is hundreds of times greater than the activity of other groups which may be covalent-linked with the drugs) on a nucleotide guanosine, thereby the the nucleic acid domain for loading the drugs is formed. Therefore, according to a size of a specific drug molecule and the number of the GC base pairs on the sequence a, the sequence b and the sequence c in the specifically designed the nucleic acid domain, when linked, a linking reaction is performed theoretically according to 1.1-1.3 times of a supersaturated linking amount, and at most 35 to 45 drugs may be linked in one the nucleic acid domain. While the above drugs are other structures, the loading amount is related to an occupation situation (including but not limited to a molecular structure, a morphology, a shape and a molecular weight) of the specific drug. Therefore, a linkage condition of an activity site of the drug and the —NH bond on the nucleotide guanosine of the the nucleic acid domain is relatively harsh, and it may also be loaded but it is relatively difficult to cause a situation of excessive linkage. According to a third aspect of the present application, a pharmaceutical composition is further provided, and the pharmaceutical composition includes any one of the above nucleic acid nanocarrier drugs. Specifically, suitable combination drugs or auxiliaries may be selected according to actual needs to form a drug combination that has combined efficacy or may improve a certain aspect of the drug properties (such as stability). 
     According to a fourth aspect of the present application, an application of any one of the above nucleic acid nanocarrier drugs in preparing a drug for treating an Alzheimer&#39;s disease, a tumor, an autoimmune disease or a heart disease. For specific applications, a new drug may be obtained by improving the drug itself on the basis of the drug of the present application, or the drug of the present application is served as a main active ingredient and prepared into a preparation in a suitable dosage form and the like. 
     Specifically, according to the different drugs in the nucleic acid nanocarrier drugs, the diseases which may be treated are also different. While the drug in the nucleic acid nanocarrier drug includes the tacrine, it may be used to prepare a drug for Alzheimer&#39;s disease. While the drug includes the epirubicin, the above nucleic acid nanocarrier drug may be used to prepare a drug for the treatment of a tumor, and the tumor may be any one of more of acute leukemia, malignant lymphoma, breast cancer, bronchial lung cancer, ovarian cancer, Wilms tumor, soft tissue sarcoma, primary hepatocellular carcinoma, metastatic liver cancer, and medullary thyroid carcinoma. 
     While the drug includes methotrexate, the above nucleic acid nanocarrier drug may be used to prepare a drug for preventing and/or treating the tumor or the autoimmune disease. Preferably, the tumor targeted is any one of more of acute leukemia, breast cancer, choriocarcinoma, malignant hydatidiform mole, head and neck tumors, bone tumors, leukemia meninges spinal cord infiltration, lung cancer, reproductive system tumors, and liver cancer, and the autoimmune disease is any one or more of refractory psoriasis, systemic lupus erythematosus, mandatory spondylitis and dermatomyositis. 
     While the drug includes the pirarubicin, the above nucleic acid nanocarrier drug may be used to prepare a drug for treating the tumor. Preferably, the tumor is any one or more of the breast cancer, head and neck cancer, bladder cancer, ureteral cancer, renal pelvis cancer, ovarian cancer and cervical cancer. 
     While the drug includes the daunorubicin, the above nucleic acid nanocarrier drug may also be used to prepare a drug for treating the tumor. Preferably, the tumor is the acute lymphocytic leukemia or granulocytic leukemia. 
     While the drug includes the pentafluorouracil, the above nucleic acid nanocarrier drug may also be used to prepare a drug for treating the tumor. Preferably, it may be used to prepare drugs for treating the liver cancer, colon cancer, rectal cancer, stomach cancer, breast cancer, ovarian cancer, choriocarcinoma, malignant hydatidiform mole, head and neck squamous cell carcinoma, skin cancer, lung cancer, cervical cancer, pancreatic cancer or bladder cancer. 
     While the drug is the 10-hydroxycamptothecin, the above nucleic acid nanocarrier drug may also be used to prepare a drug for treating the liver cancer, stomach cancer, head and neck cancer or leukemia. 
     While the drug is the aspirin, the above nucleic acid nanocarrier drug may be used to prepare drugs for antipyretic and analgesic, preventing the heart disease and cerebral thrombosis, anti-inflammatory and anti-rheumatic, treating arthritis, alleviating skin mucosal lymph node syndrome in a patient with a Kawasaki disease, resisting the cancer, and preventing digestive tract tumor. 
     While the drug includes the gemcitabine, the above nucleic acid nanocarrier drug may also be used to prepare a drug for treating the tumor. Preferably, it may be used to prepare drugs for treating the pancreatic cancer, non-small cell lung cancer, ovarian cancer, breast cancer, bladder cancer, cervical cancer, liver cancer, biliary tract cancer, nasopharyngeal cancer, testicular tumor, lymphoma, mesothelioma or head and neck cancer. 
     According to a fifth aspect of the present application, a method for preventing and/or treating an Alzheimer&#39;s disease, a tumor, an autoimmune disease or a heart disease is further provided, the method includes: any one of the above nucleic acid nanocarrier drugs or pharmaceutical compositions is provided; and an effective dosage of the above nucleic acid nanocarrier drug or pharmaceutical composition is administered to a patient with the Alzheimers disease, the tumor, the autoimmune disease or the heart disease. The effective dosage herein includes a prophylactically effective dosage and/or a therapeutically effective dosage. The therapeutically effective dosage refers to a dosage that is effective to achieve a desired therapeutic result, such as a reduction of the Alzheimer&#39;s disease, within a necessary dosage and time period. In a specific implementation mode, the dosage may be adjusted to provide the optimal therapeutic response dosage, and the therapeutically effective dosage may be varied according to the following factors: a disease state, an age, a gender, and a weight of an individual and an ability of a preparation which causes a desired response in the individual. The meaning of the therapeutically effective dosage also includes a dosage of which beneficial effects of treatment exceed its toxic or harmful effects. The prophylactically effective dosage refers to a dosage that is effective to achieve the desired preventive result, such as preventing or inhibiting the occurrence of the Alzheimers disease, within the necessary dosage and time period. The prophylactically effective dosage may be determined according to the above description of the therapeutically effective dosage. For any specific subjects, the specific dosage may be adjusted along with time according to individual needs and the professional judgment of an administering person. 
     It is to be noted that the nucleic acid nanoparticle formed by the se-assembly of the sequences or sequence variations provided by the present application may also be used as basic structural units, and may be further polymerized to form multimers, such as a dimer, a trimer, a tetramer, a pentamer, a hexamer or a heptamers, according to the actual application needs. 
     The beneficial effects of the present application are further described below in combination with specific embodiments. 
     Assembly of Nucleic Acid Nanoparticles 
     Embodiment 1 
     I. RNA and DNA Nanoparticle Carrier: 
     (1) Three polynucleotide base sequences for assembling RNA nanoparticles are specifically shown in Table 1: 
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                 Sequence sense 
                 Base 
                 Molecular 
                 Chemical 
                 Fluorescence 
                 Special 
                 molecular 
               
               
                 Name 
                 (5′-3′) 
                 number 
                 weight 
                 modification 
                 mark 
                 modification 
                 weight 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 a-strand (SEQ ID NO: 52) 
                 cGcGcGcccAccAGc GuuccGGGcGccGc 
                 29 
                 9678.7 
                 C/U base 2′F modification 
                 / 
                 5′Biotin 
                 29524.9 
               
               
                   
               
               
                 b-strand (SEQ ID NO: 53) 
                 GcGGcGcccGGuuc GccGccAGGcGGc 
                 27 
                 9116.8 
                 C/U base 2′F modification 
                 / 
                 5′Biotin 
                   
               
               
                   
               
               
                 c-strand (SEQ ID NO: 54) 
                 GccGccAGGcGGcc AuAGcGGuGGGcG cGcG 
                 31 
                 10729.4 
                 C/U base 2′F modification 
                 5′/CY3 
               
               
                   
               
            
           
         
       
     
     (2) Three polynucleotide base sequences of DNA nanoparticles 
     DNA uses the same sequence as the above RNA, except that U is replaced by T. Herein, a molecular weight of the a-strand is 8802.66, a molecular weight of the b-strand is 8280.33, and a molecular weight of the c-strand is 9605.2. 
     The strands a, b and c of the above RNA nanoparticles and DNA nanoparticles are all commissioned to be synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. 
     II. Set-Assembly Experiment Steps: 
     (1) according to a molar ratio of 1:1:1, enabling the RNA or DNA single strands a, b and c to be simultaneously mixed and dissolved in DEPC treated water or TMS buffer solution; 
     (2) heating mixed solution to 80/95 DEG C. (herein a RNA assembly temperature is 80 DEG C., and a DNA assembly temperature is 95 DEG C.), after keeping for 5 min, slowly cooling to a room temperature at a rate of 2 DEG C./min; 
     (3) enabling a product to be loaded on 8% (m/v) of non-denaturing PAGE gel and placed in TBM buffer solution under a condition of 4 DEG C., purifying a complex by 100 v of electrophoresis; 
     (4) cutting a target band and eluting in RNA/DNA elution buffer solution at 37 DEG C., after that, precipitating overnight in ethanol, volatilizing under reduced pressure and low temperature, to obtain a self-assembly product; and 
     (5) electrophoresis analysis detection and laser scanning observation. 
     III. Self-Assembly Experiment Result 
     Electrophoresis Detection Result 
     An electrophoresis detection result of the RNA self-assembly product is shown in  FIG. 1 . In  FIG. 1 , lanes 1 to 3 from left to right are successively: the a-strand, the b-strand, and the RNA set-assembly product. It may be seen from the figure that although the RNA self-assembly product is slightly diffused, it may be apparently seen that it is a single band. In addition, because the molecular weight is a molecular weight after assembly, it is larger than a single-stranded molecular weight, a band position is behind the a-strand and the b-strand, and the actual situation is consistent with a theory, it is proved that a stable composite structure is formed between the above RNA single strands by the set-assembly, and RNA nanoparticles are formed. 
     An electrophoresis detection result of the DNA self-assembly product is shown in  FIG. 2 . In  FIG. 2 , lanes 1 to 3 from left to right are successively: the a-strand, the b-strand, and the DNA self-assembly product. It may be seen from the figure that a band of the DNA self-assembly product is bright and clear, and is a single band, it is proved that a stable composite structure is formed between the above DNA single strands by the self-assembly, and DNA nanoparticles are formed. 
     In the embodiment, it is verified by gel electrophoresis that the sequences a, b and c including the RNA core sequences SEQ ID NO:1, SEQ ID NO:3 and SEQ ID NO:5 may be successfully self-assembled into the RNA nanoparticles. The sequences a, b and c including the DNA core sequences SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6 may also be successfully self-assembled into the DNA nanoparticles. 
     In addition to the core sequences which form a nucleic acid structural domain, the sequences a, b and c of the above RNA nanoparticles and DNA nanoparticles also have various extension sequences (including a drug loading linking sequence) which promote a loading function of the nucleic acid structural domain and a target head or a fluorescein which is linked with the nucleic acid structural domain. It may be seen that the presence of substances other than these core sequences does not affect the formation of the nucleic acid structural domain and the successful self-assembly of the nucleic acid nanoparticles. The self-assembled nucleic acid nanoparticles may have a targeting property under the guidance of the target head, and the fluorescein may make the nucleic acid nanoparticles have visibility and traceability. 
     Embodiment 2 
     I. 7 Groups of Short-Sequence RNA Nanoparticle Carriers: 
     (1) 7 groups of three polynucleotide base sequences for forming RNA nanoparticles are shown in Table 2 to Table 8: 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 R-1: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                 Sequence sense 
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a-strand (SEQ ID NO: 55) 
                   GG AGcGuu GG   
                 10 
                 c/u base 2′F modification 
                 3262.9 
                 9828.7 
               
               
                   
               
               
                 b-strand (SEQ ID NO: 56) 
                 ccuuc GCCG   
                 9 
                 c/u base 2′F modification 
                 2780.6 
                   
               
               
                   
               
               
                 c-strand (SEQ ID NO: 57) 
                 c GG ccAuAGccc 
                 12 
                 c/u base 2′F modification 
                 3785.2 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 R-2: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                 Sequence sense 
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a-strand (SEQ ID NO: 58) 
                   G cAGcGuuc G   
                 10 
                 c/u base 2′F modification 
                 3186.7 
                 9829.4 
               
               
                   
               
               
                 b-strand (SEQ ID NO: 59) 
                 c G uuc G cc G   
                 9 
                 c/u base 2′F modification 
                 2820.2 
                   
               
               
                   
               
               
                 c-strand (SEQ ID NO: 60) 
                 c GG ccAuAGc G c 
                 12 
                 c/u base 2′F modification 
                 3822.5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 R-3: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                 Sequence sense 
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a-strand (SEQ ID NO: 61) 
                 c G AGcGuu G c 
                 10 
                 c/u base 2′F modification 
                 3187.5 
                  9829.9 
               
               
                   
               
               
                 b-strand (SEQ ID NO: 62) 
                 Gcuuc G cc G   
                 9 
                 c/u base 2′F modification 
                 2819.2 
                   
               
               
                   
               
               
                 c-strand (SEQ ID NO: 63) 
                 c GG ccAuAGcc G   
                 12 
                 c/u base 2′F modification 
                 3823.2 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 R-4: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                 Sequence sense 
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a-strand (SEQ ID NO: 64) 
                   GG AGcGuu GG   
                 10 
                 c/u base 2′F modification 
                 3263.7 
                 9830.9 
               
               
                   
               
               
                 b-strand (SEQ ID NO: 65) 
                 ccuuc GGGG   
                 9 
                 c/u base 2′F modification 
                 2858.2 
                   
               
               
                   
               
               
                 c-strand (SEQ ID NO: 66) 
                 cccccAuAGccc 
                 12 
                 c/u base 2′F modification 
                 3709.0 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 R-5: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                 Sequence sense 
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a-strand (SEQ ID NO: 67) 
                   G cAGcGuuc G   
                 10 
                 c/u base 2′F modification 
                 3187.5 
                 9830.2 
               
               
                   
               
               
                 b-strand (SEQ ID NO: 68) 
                 c G uuc GG c G   
                 9 
                 c/u base 2′F modification 
                 2857.5 
                   
               
               
                   
               
               
                 c-strand (SEQ ID NO: 69) 
                 c G cccAuAGc G c 
                 12 
                 c/u base 2′F modification 
                 3785.2 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 R-6: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                 Sequence sense 
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a-strand (SEQ ID NO: 70) 
                   G cAGcGuuc G   
                 10 
                 c/u base 2′F modification 
                 3187.2 
                 9830.5 
               
               
                   
               
               
                 b-strand (SEQ ID NO: 71) 
                 c G uuc GG cc 
                 9 
                 c/u base 2′F modification 
                 2820.4 
                   
               
               
                   
               
               
                 c-strand (SEQ ID NO: 72) 
                   GG cccAuAGc G c 
                 12 
                 c/u base 2′F modification 
                 3822.9 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 R-7: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                 Sequence sense 
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a-strand (SEQ ID NO: 73) 
                 c G AGcGuu G c 
                 10 
                 c/u base 2′F modification 
                 3187.6 
                 9830.7 
               
               
                   
               
               
                 b-strand (SEQ ID NO: 74) 
                   G cuuc GG c G   
                 9 
                 c/u base 2′F modification 
                 2857.8 
                   
               
               
                   
               
               
                 c-strand (SEQ ID NO: 75) 
                 c G cccAuAGccG 
                 12 
                 c/u base 2′F modification 
                 3785.3 
               
               
                   
               
            
           
         
       
     
     The single strands of the above 7 groups of the short-sequence RNA nanoparticle carriers are all commissioned to be synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. 
     II. Self-Assembly Experiment Steps: 
     (1) according to a molar ratio of 1:1:1, enabling the RNA single strands a, b and c to be simultaneously mixed and dissolved in DEPC treated water or TMS buffer solution; 
     (2) heating mixed solution to 80 DEG C., after keeping for 5 min, slowly cooling to a room temperature at a rate of 2 DEG C./min; 
     (3) enabling a product to be loaded on 8% (m/v) of non-denaturing PAGE gel and placed in TBM buffer solution under a condition of 4 DEG C., purifying a complex by 100 v of electrophoresis; 
     (4) cutting a target band and eluting in RNA elution buffer solution at 37 DEG C., after that, precipitating overnight in ethanol, volatilizing under reduced pressure and low temperature, to obtain a short-sequence RNA self-assembly product; 
     (5) electrophoresis analysis detection and laser scanning observation; and 
     (6) electric potential detection. 
     III. Self-Assembly Experiment Result 
     (1) Electrophoresis Detection Result 
     A 2% agarose gel electrophoresis diagram of the 7 groups of the short-sequence RNA self-assembly products is shown in  FIG. 3 . From left to right, lanes 1 to 7 in  FIG. 3  are successively: short-sequences R-1, R-2, R-3, R-4, R-5, R-6 and R-7. 
     A 4% agarose gel electrophoresis diagram of the 7 groups of the short-sequence RNA self-assembly products is shown in  FIG. 4 . From left to right, lanes 1 to 7 in  FIG. 4  are successively: short-sequences R-1, R-2, R-3, R-4, R-5, R-6 and R-7. 
     It may be clearly seen from results of  FIG. 3  and  FIG. 4  that bends of the R-2, R-3, R-5 and R-7 in the 7 groups of the short-sequence self-assembly products are bright and clear, although the R-1, R-4 and R-6 are relatively diffused, it may still be seen as a single band, it is indicated that the 7 groups of the short-sequences may be self-assembled better into a RNA nanoparticle structure. 
     (2) Electric Potential Measurement 
     Measurement method: preparing a potential sample (the self-assembly product is dissolved in ultrapure water) and putting into a sample pool, opening a sample pool cover of an instrument, and placing the instrument; 
     opening software, clicking a menu MeasUre€ManUal, so a manual measurement parameter setting dialog box appears; 
     setting a software detection parameter; 
     and then clicking Ok to complete the settings, after a measurement dialog box appears, clicking start to start. 
     Measurement result, potential detection results of the 7 groups of the short-sequence RNA nanoparticles are shown below in Table 9 to Table 15: 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 9 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                 Potential  
               
               
                   
                 name 
                 times 
                 ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-1 
                 1 
                 −33.7 
               
               
                   
                   
                 2 
                 −35.6 
               
               
                   
                   
                 3 
                 −34.6 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −34.65 mV. (±0.95 mV) 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 10 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                 Potential  
               
               
                   
                 name 
                 times 
                 ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-2 
                 1 
                 −37.9 
               
               
                   
                   
                 2 
                 −37.3 
               
               
                   
                   
                 3 
                 −36.8 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −37.35 mV. (±0.55 mV) 
               
               
                   
                 result 
                   
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 11 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                 Potential  
               
               
                   
                 name 
                 times 
                 ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-3 
                 1 
                 −31.5 
               
               
                   
                   
                 2 
                 −33 
               
               
                   
                   
                 3 
                 −32.7 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −32.425 mV. (±0.75 mV) 
               
               
                   
                 result 
                   
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 12 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                 Potential  
               
               
                   
                 name 
                 times 
                 ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-4 
                 1 
                 −35.40  
               
               
                   
                   
                 2 
                 −36.00 
               
               
                   
                   
                 3 
                 −35.50 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −35.7 mV. (±0.3 mV) 
               
               
                   
                 result 
                   
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 13 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                 Potential  
               
               
                   
                 name 
                 times 
                 ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-5 
                 1 
                 −34.00 
               
               
                   
                   
                 2 
                 −33.30 
               
               
                   
                   
                 3 
                 −34.90 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −34.1 mV. (±0.8 mV) 
               
               
                   
                 result 
                   
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 14 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                 Potential 
               
               
                   
                 name 
                 times 
                 ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-6 
                 1 
                 −33.10 
               
               
                   
                   
                 2 
                 −36.10 
               
               
                   
                   
                 3 
                 −35.60 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −34.6 mV. (±1.5 mV) 
               
               
                   
                 result 
                   
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 15 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                 Potential  
               
               
                   
                 name 
                 times 
                 ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-7 
                 1 
                 −35.60 
               
               
                   
                   
                 2 
                 −34.80 
               
               
                   
                   
                 3 
                 −34.00 
               
            
           
           
               
               
               
            
               
                   
                 Experiment  
                 −34.80 mV. ±0.8 mV 
               
               
                   
                 result 
                   
               
               
                   
                   
               
            
           
         
       
     
     It may be seen from the above potential detection data that the 7 groups of the short-sequence RNA set-assembly products all have good stability, and it is further indicated that the nanoparticles formed by the self-assembly of each short-sequence RNA have a relatively stable set-assembly structure. 
     It is indicated from the embodiment that: different combinations of the core sequences a, b and c may form the RNA nanoparticles with the nucleic acid structural domain through the self-assembly, and the structure is stable. It may be seen on the basis of Embodiment 1 that the RNA nanoparticles may also be successfully assembled by adding various functional extension fragments or a linkage target head, a fluorescein and the like on the basis of these different core sequence combinations, and have functions such as drug loading, cell targeting, visibility and traceability. 
     In order to further verify these performances, the extension fragment is added on the basis of Embodiment 2, and it is specifically described in Embodiment 3. On the basis of the DNA core sequence corresponding to the RNA core sequence of Embodiment 2, the extension fragment is added, and the target head is connected or unconnected at the same time, it is specifically described in Embodiment 4. 
     Embodiment 3 
     I. 7 Groups of Conventional-Sequence RNA Nanoparticle Carriers: 
     (1) 7 groups of three polynucleotide base sequences for forming RNA nanoparticles are shown in Table 16 to Table 22: 
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 R-8 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 76) 
                 cGcGcGcccA GG AGcGu u GG cGGGcGGcG 
                 29 
                 C/U 2′F modification 
                 9462.78 
                 28084.13 
               
               
                 b (SEQ ID NO: 77) 
                 cGccGcccGccuuc G cc G c cAGccGcc 
                 27 
                 C/U 2′F modification 
                 8522.18 
                   
               
               
                 c (SEQ ID NO: 78) 
                 GGcGGcAGGc GG ccAu AGcccuGGGcGcGcG 
                 31 
                 C/U 2′F modification 
                 10099.17 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 R-9 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 79) 
                 cGcGcGcccA G cAGcGuu c G cGGGcGGcG 
                 29 
                 C/U 2′F modification 
                 9386.73 
                 28084.13 
               
               
                   
               
               
                 b (SEQ: ID NO: 80) 
                 cGccGcccGc G uuc G cc G  ccAGccGcc 
                 27 
                 C/U 2′F modification 
                 8560.20 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 81) 
                 GGcGGcAGGc GG ccAu AGcGcuGGGcGcGcG 
                 31 
                 C/U 2′F modification 
                 10137.20  
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                 R-10 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 82) 
                 cGcGcGccAc G AGcGuu  G cGGGGcGGcG 
                 29 
                 C/U 2′F modification 
                 9424.75 
                 28084.13 
               
               
                   
               
               
                 b (SEQ ID NO: 83) 
                 cGccGcccc G cuuc G cc G c cAGccGcc 
                 27 
                 C/U 2′F modification 
                 8522.18 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 84) 
                 GGcGGcAGGc GG ccAu AGcc G uGGGcGcGcG 
                 31 
                 C/U 2′F modification 
                 10137.20 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 19 
               
             
            
               
                   
               
               
                 R-11 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 85) 
                 cGcGcGcccA GG AGCGu u GG cccGcGGcG 
                 29 
                 C/U 2′F modification 
                 9386.73 
                 28084.13 
               
               
                   
               
               
                 b (SEQ: ID NO: 86) 
                 cGccGcGGGccuuc GGG   G ccAGccGcc 
                 27 
                 C/U 2′F modification 
                 8674.28 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 87) 
                 GGcGGcAGGcccccAuA GcccuGGGcGcGcG 
                 31 
                 C/U 2′F modification 
                 10023.12 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 20 
               
             
            
               
                   
               
               
                 R-12 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
               
                 a (SEQ ID NO: 88) 
                 cGcGcGcccA G cAGcGuu c G ccccGccGc 
                 29 
                 C/U 2′F modification 
                 9234.62 
                 28084.18 
               
               
                   
               
               
                 b (SEQ ID NO: 89) 
                 GcGGcGGGGc G uuc GG  c G GcAGGcGGc 
                 27 
                 C/U 2′F modification 
                 8864.41 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 90) 
                 GccGccAGcc G cccAuAG c G cuGGGcGcGcG 
                 31 
                 C/U 2′F modification 
                 9985.10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 21 
               
             
            
               
                   
               
               
                 R-13 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 91) 
                 cGcGcGcccA G cAGcGuu c G GGGcGccGc 
                 29 
                 C/U 2′F modification 
                 9348.70 
                 28736.53 
               
               
                   
               
               
                 b (SEQ ID NO: 92) 
                 GcGGcGcccc G uuc GG cc GGcAGGcGGc 
                 28 
                 C/U 2′F modification 
                 9057.52 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 93) 
                 GccGccAGcc GG cccAuA Gc G cuGGGcGcGcG 
                 32 
                 C/U 2′F modification 
                 10330.31 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 22 
               
             
            
               
                   
               
               
                 R-14 ( uGAcAGAuAAGGAAccuGcudTdT  in the following 
               
               
                 a-strand is survivin siRNA) 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 94) 
                 cGcGcGc G AGcGuu G cA A uGAcAGAuAAGGAAcc   uGcudTdT   
                 39 
                 C/U 2′F modification 
                 12901.91 
                 31832.42 
               
               
                   
               
               
                 b (SEQ ID NO: 95) 
                   GGcAGGuuccuuAucuGu   cA AA G cuuc GG c G GcAG c 
                 36 
                 C/U 2′F modification 
                 11555.97 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 96) 
                 GcAGcc G cccAuAGcc G c GcGcG 
                 23 
                 C/U 2‘F modification 
                 7374.54 
               
               
                   
               
            
           
         
       
     
     The single strands of the above 7 groups of conventional-sequence RNA nanoparticle carriers are all commissioned to be synthesized by Suzhou Gima Company, herein the sequence a, the sequence b and the sequence c in R-8 to R-14 are respectively extended RNA oligonucleotide sequences formed by adding the extension fragments on the basis of the sequence a, the sequence b and the sequence c in R-1 to R-7, a targeting module fragment is not extended, and C/U base 2′F modification (resistance to enzyme digestion and stability are enhanced) is performed. In addition, a siRNA nucleic acid interference therapeutic fragment of a survivin is modified in the above RNA nanoparticle R-14, specifically a sense strand (see an underlined part of the a-strand) of Survivin siRNA is extended at a-strand 3′-end, and an antisense strand (see an underlined part of the b-strand) is extended and connected at b-strand 5′-end, so base pair complementary is formed. 
     II. Self-Assembly Experiment Steps: 
     (1) according to a molar ratio of 1:1:1, enabling the RNA single strands a, b and c to be simultaneously mixed and dissolved in DEPC treated water or TMS buffer solution; 
     (2) heating mixed solution to 80 DEG C., after keeping for 5 min, slowly cooling to a room temperature at a rate of 2 DEG C./min; 
     (3) enabling a product to be loaded on 8% (m/v) of non-denaturing PAGE gel and placed in TBM buffer solution under a condition of 4 DEG C., purifying a complex by 100 v of electrophoresis; 
     (4) cutting a target bend and eluting in RNA elution buffer solution at 37 DEG C., after that, precipitating overnight in ethanol, volatilizing under reduced pressure and low temperature; 
     (5) electrophoresis analysis detection and laser scanning observation; 
     (6) electric potential detection. 
     III. Self-Assembly Experiment Result 
     (1) Electrophoresis Detection Result 
     A 2% agarose gel electrophoresis diagram of the 7 groups of the conventional-sequence RNA self-assembly products is shown in  FIG. 5 . From left to right, lanes 1 to 7 in  FIG. 5  are successively: conventional-sequence RNA self-assembly products R-8, R-9, R-10, R-11, R-12, R-13 and R-14. 
     A 4% agarose gel electrophoresis diagram of the 7 groups of the conventional-sequence RNA self-assembly products is shown in  FIG. 6 . From left to right, lanes 1 to 7 in  FIG. 6  are successively: conventional-sequence RNA self-assembly products R-8, R-9, R-10, R-11, R-12, R-13 and R-14. 
     It may be dearly seen from results of  FIG. 5  and  FIG. 6  that bands of the 7 groups of the conventional-sequence self-assembly products are bright and clear single bands, it is indicated that the 7 groups of the conventional-sequences may be self-assembled into a nanostructure. Herein after a Survivin siRNA nucleic acid interference therapeutic fragment is modified in the conventional-sequence RNA self-assembly product R-14, it still has the stable self-assembly structure, it is also indicated that the nucleic acid nanoparticles of the disclosure may load a nucleic acid drug, and have a delivery carrier function of the nucleic acid drug. 
     (2) Electric Potential Measurement 
     Measurement method: preparing a potential sample (the self-assembly product is dissolved in ultrapure water) and putting into a sample pool, opening a sample pool cover of an instrument, and placing the instrument; 
     opening software, clicking a menu MeasUre€ManUal, so a manual measurement parameter setting dialog box appears; 
     setting a software detection parameter; 
     and then clicking Ok to complete the settings, after a measurement dialog box appears, clicking Start to start. 
     Measurement result: potential detection results of the 7 groups of the conventional-sequence RNA nanoparticles are shown below in Table 23 to Table 29: 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 23 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                 Potential  
               
               
                   
                 name 
                 times 
                 ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-8 
                 1 
                 −18.00 
               
               
                   
                   
                 2 
                 −16.10 
               
               
                   
                   
                 3 
                 −18.20 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −17.43 mV (±1.33 mV) 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 24 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP 
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 R-9 
                 1 
                 −16.90 
               
               
                   
                 2 
                 −17.50 
               
               
                   
                 3 
                 −20.20 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −18.20 mV (±1.3 mV) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 25 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP 
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 R-10 
                 1 
                 −10.40 
               
               
                   
                 2 
                 −11.80 
               
               
                   
                 3 
                 −10.40 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −10.87 mV (±0.47 mV) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 26 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP 
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 R-11 
                 1 
                 −28.30 
               
               
                   
                 2 
                 −26.00 
               
               
                   
                 3 
                 −33.10 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −29.13 mV (±3.13 mV) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 27 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP 
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 R-12 
                 1 
                 −7.59 
               
               
                   
                 2 
                 −7.80 
               
               
                   
                 3 
                 −17.20 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −10.86 mV (±3.27 mV) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 28 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP  
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 R-13 
                 1 
                 −9.64 
               
               
                   
                 2 
                 −15.60 
               
               
                   
                 3 
                 −21.10 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −15.45 mV (±5.81 mV) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 29 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP  
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 R-14 
                 1 
                 −21.40 
               
               
                   
                 2 
                 −21.20 
               
               
                   
                 3 
                 −28.00 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −23.53 mV. ± 2.33 mV 
               
               
                   
               
            
           
         
       
     
     It may be seen from the above potential detection data that the 7 groups of the conventional-sequence RNA set-assembly products all have good stability, and it is further indicated that the nanoparticles formed by the self-assembly of each conventional-sequence RNA have a relatively stable self-assembly structure. 
     It is indicated from the embodiment that: on the basis of different combinations of the RNA core sequences, the RNA nanoparticles with the stable structure may also be successfully self-assembled by adding the extension fragments. At the same time, the added extension fragments make the RNA nanoparticles have superior drug loading performance (specifically described in Embodiment 5). 
     Embodiment 4 
     I. 7 Groups of Conventional-Sequence DNA Nanoparticle Carriers: 
     (1) 7 groups of three polynucleotide base sequences for forming DNA nanoparticles are shown below in Table 30 to Table 36. 
     An EGFRapt target head or a PSMAapt(A9L) target head is extended in a part of a-strands in the table: 
     
       
         
           
               
               
            
               
                   
                 EGFRapt (SEQ ID NO: 97): 
               
               
                   
                 GCCTTAGTAACGTGCTTTGATGTCGATTCGACAGGAGGC; 
               
               
                   
                   
               
               
                   
                 PSMAapt (A9L, SEQ ID NO: 98): 
               
               
                   
                 GGGCCGAAAAAGACCTGACTTCTATACTAAGTCTACGTCCC. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 30 
               
             
            
               
                   
               
               
                 D-1 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 99) 
                 CGCGCGCCCAGGAGCGTTGGC GGGCGGCG GCCTTAGTAACGTG   CTTTGATGTCGATTCGACAGGA   GGC   
                 68 
                 3 bases before 5′-end and 3′-end, thio modification 
                 21214.63 
                 39092.09 
               
               
                   
               
               
                 b (SEQ ID NO: 100) 
                 CGCCGCCCGCCTTCGCCGCCA GCCGCC 
                 27  
                 3 bases before 5′-end and 3′-end, thio modification 
                 8176.24  
                   
               
               
                   
               
               
                 c (SEQ ID NO: 101) 
                 GGCGGCAGGCGGCCATAGCCC TGGGCGCGCG 
                 31 
                 3 bases before 5′-end and 3′-end, thio modification 
                 9701.22 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 31 
               
             
            
               
                   
               
               
                 D-2 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 102) 
                 CGCGCGCCCAGCAGCGTTCGC GGGCGGCG GCCTTAGTAACGTG   CTTTGATGTCGATTCGACAGGA   GGC   
                 68 
                 3 bases before 5′-end and 3′-end, thio modification 
                 21134.59 
                 39092.11 
               
               
                   
               
               
                 b (SEQ ID NO: 103) 
                 CGCCGCCCGCGTTCGCCGCCA GCCGCC 
                 27 
                 3 bases before 5′-end and 3′-end, thio modification 
                 8216.27 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 104) 
                 GGCGGCAGGCGGCCATAGCGC TGGGCGCGCG 
                 31 
                 3 bases before 5′-end and 3′-end, thio modification 
                 9741.25 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 32 
               
             
            
               
                   
               
               
                 D-3 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 105) 
                 CGCGCGCCCACGAGCGTTGCG GGGCGGCG GCCTTAGTAACGTG   CTTTGATGTCGATTCGACAGGA   GGC   
                 68 
                 3 bases before 5′-end and 3′-end, thio modification 
                 21174.60 
                 39092.09 
               
               
                   
               
               
                 b (SEQ ID NO: 106) 
                 CGCCGCCCCGCTTCGCCGCCA GCCGCC 
                 27 
                 3 bases before 5′-end and 3′-end, thio modification 
                 8176.24 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 107) 
                 GGCGGCAGGCGGCCATAGCCG TGGGCGCGCG 
                 31 
                 3 bases before 5′-end and 3′-end, thio modification 
                 9741.25 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 33 
               
             
            
               
                   
               
               
                 D-4 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 108) 
                 CGCGCGCCCAGGAGCGTTGGCC CGCGGCGT GGGCCGAAAAAGAC   CTGACTTCTATACTAAGTCTACGT   CCC   
                 71 
                 3 bases before 5′-end and 3′-end, thio modification 
                 21923.12 
                 39780.63 
               
               
                   
               
               
                 b (SEQ  ID NO: 109) 
                 CGCCGCGGGCCTTCGGGGCCAG CCGCC 
                 27 
                 3 bases before 5′-end and 3′-end, thio modification 
                 8236.34 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 110) 
                 GGCGGCAGGCCCCCATAGCCCT GGGCGCGCG 
                 31 
                 3 bases before 5′-end and 3′-end, thio modification 
                 9621.17 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 34 
               
             
            
               
                   
               
               
                 D-5 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 111) 
                 CGCGCGCCCAGCAGCGTTCGCC CCGCCGCT GGGCCGAAAAAGAC   CTGACTTCTATACTAAGTCTACGT   CCC   
                 71 
                 3 bases before 5′-end and 3′-end, thio modification 
                 21763.03 
                 39880.64 
               
               
                   
               
               
                 b (SEQ ID NO: 112) 
                 GCGGCGGGGCGTTCGGCGGCAG GCGGC 
                 27 
                 3 bases before 5′-end and 3′-end, thio modification 
                 8536.46 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 113) 
                 GCCGCCAGCCGCCCATAGCGCT GGGCGCGCG 
                 31 
                 3 bases before 5′-end and 3′-end, thio modification 
                 9581.15 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 35 
               
             
            
               
                   
               
               
                 D-6 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
               
                 a (SEQ ID NO: 114) 
                 CGCGCGCCCAGCAGCG TTCGGGGCGCCGC 
                 29 
                 3 bases before 5′-end and 3′-end, thio modification 
                 8978.76 
                 27594.69 
               
               
                   
               
               
                 b (SEQ ID NO: 115) 
                 GCGGCGCCCCGTTCGG CCGGCAGGCGGC 
                 28 
                 3 bases before 5′-end and 3′-end, thio modification 
                 8705.57 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 116) 
                 GCCGCCAGCCGGCCCA TAGCGCTGGGCGCGCG 
                 32 
                 3 bases before 5′-end and 3′-end, thio modification 
                 9910.36 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 36 
               
             
            
               
                   
               
               
                 D-7 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 117) 
                 CGCGCGCCCACGAGCGTT GCGGGCGCCGC 
                 29 
                 3 bases before 5′-end and 3′-end, thio modification 
                 8978.76 
                 26976.30 
               
               
                   
               
               
                 b (SEQ ID NO: 118) 
                 GCGGCGCCCGCTTCGGCG GCAGGCGGC 
                 27 
                 3 bases before 5′-end and 3′-end, thio modification 
                 8416.39 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 119) 
                 GCCGCCAGCCGCCCATAG CCGTGGGCGCGCG 
                 31 
                 3 bases before 5′-end and 3′-end, thio modification 
                 9581.15 
               
               
                   
               
            
           
         
       
     
     Single strands of the above 7 groups of the conventional-sequence DNA nanoparticles are all commissioned to be synthesized by Suzhou Hongxun Company, herein: 
     D-1 is the conventional-sequence DNA nanoparticles formed after adding an extension sequence containing the EGFRapt target head (see an underlined part) on the basis of the above core sequences (8) (sequence a: 5′-GGAGCGTTGG-3′, sequence b: 5′-CCTTCGCCG-3′, and sequence c: 5′-CGGCCATAGCCC-3); 
     D-2 is the conventional-sequence DNA nanoparticles formed after adding an extension sequence containing the EGFRapt target head (see an underlined part) on the basis of the above core sequences (9) (sequence a: 5′-GCAGCGTTCG-3′, sequence b: 5′-CGTTCGCCG-3′, and sequence c: 5′-CGGCCATAGCGC-3′); 
     D-3 is the conventional-sequence DNA nanoparticles formed after adding an extension sequence containing the EGFRapt target head (see an underlined part) on the basis of the above core sequences (10) (sequence a: 5′-CGAGCGTTGC-3′, sequence b: 5′-GCTTCGCCG-3′, and sequence c: 5′-CGGCCATAGCCG-3′); 
     D-4 is the conventional-sequence DNA nanoparticles formed after adding an extension sequence containing the PSMAapt target head (see an underlined part) on the basis of the above core sequences (11) (sequence a: 5′-GGAGCGTTGG-3′, sequence b: 5′-CCTTCGGGG-3′, and sequence c: 5′-CCCCCATAGCCC-3′); 
     D-5 is the conventional-sequence DNA nanoparticles formed after adding an extension sequence containing the PSMAapt target head (see an underlined part) on the basis of the above core sequences (12) (sequence a: 5′-GCAGCGTTCG-3′, sequence b: 5′-CGTTCGGCG-3′, and sequence c: 5′-CGCCCATAGCGC-3′); 
     D-6 is the conventional-sequence DNA nanoparticles formed after adding an extension sequence without containing a target head structure on the basis of the above core sequences (13) (sequence a: 5′-GCAGCGTTCG-3′, sequence b: 5′-CGTTCGGCC-3′, and sequence c: 5′-GGCCCATAGCGC-3′); and 
     D-7 is the conventional-sequence DNA nanoparticles formed after adding an extension sequence without containing a target head structure on the basis of the above core sequences (14) (sequence a: 5′-CGAGCGTTGC-3′, sequence b: 5′-GCTTCGGCG-3′, and sequence c: 5′-CGCCCATAGCCG-3′). 
     II. Self-Assembly Experiment Steps: 
     (1) According to a molar ratio of 1:1:1, enabling the DNA single strands a, b and c to be simultaneously mixed and dissolved in DEPC treated water or TMS buffer solution; 
     (2) heating mixed solution to 95 DEG C., after keeping for 5 min, slowly cooling to a room temperature at a rate of 2 DEG C./min; 
     (3) enabling a product to be loaded on 8% (m/v) of non-denaturing PAGE gel and placed in TBM buffer solution under a condition of 4 DEG C., purifying a complex by 100 v of electrophoresis; 
     (4) cutting a target band and eluting in DNA elution buffer solution at 37 DEG C., after that, precipitating overnight in ethanol, volatilizing under reduced pressure and low temperature, to obtain a conventional-sequence DNA self-assembly product; 
     (5) electrophoresis analysis detection and laser scanning observation; 
     (6) electric potential detection; 
     (7) particle size measurement; and 
     (8) observing by a transmission electron microscope. 
     III. Self-Assembly Experiment Result 
     (1) Electrophoresis Detection Result 
     A 2% agarose gel electrophoresis diagram of the 7 groups of the conventional-sequence DNA set-assembly products is shown in  FIG. 7 . From left to right, lanes 1 to 7 in  FIG. 7  are successively: conventional-sequence DNA self-assembly products D-1. D-2, D-3, D-4, D-5, D-6 and D-7. 
     A 4% agarose gel electrophoresis diagram of the 7 groups of the conventional-sequence DNA self-assembly products is shown in  FIG. 8 . From left to right, lanes 1 to 7 in  FIG. 8  are successively: conventional-sequence DNA self-assembly products D-1. D-2, D-3, D-4, D-5, D-6 and D-7. 
     It may be dearly seen from results of  FIG. 7  and  FIG. 8  that bands of the 7 groups of the conventional-sequence DNA self-assembly products are bright and clear, it is indicated that the 7 groups of the conventional-sequence DNA strands are all set-assembled to form stable nanoparticle structures. Herein two groups of the self-assembly structures D-6 and D-7 carry the EGFRapt or PSMAapt target head, and the molecular weights are slightly low, positions of bands thereof are apparently in front of the other bands, actual and theoretical conditions are completely consistent, so the stability of the self-assembly structures is further proved. 
     It is indicated from the embodiment on the base of combinations of these different DNA core sequences, when various functional extension fragments are added or the target head is linked at the same time, the DNA nanoparticles may also be successfully assembled, and also have functions such as drug loading, cell targeting, visibility and traceability (specifically described in Embodiment B and Embodiment 8). 
     (2) Electric Potential Measurement 
     Measurement method: preparing a potential sample (the self-assembly product is dissolved in ultrapure water) and putting into a sample pool, opening a sample pool cover of an instrument, and placing the instrument; 
     opening software, clicking a menu MeasUre€ManUal, so a manual measurement parameter setting dialog box appears; 
     setting a software detection parameter; 
     and then clicking Ok to complete the settings, after a measurement dialog box appears, clicking Start to start. 
     Measurement result: potential detection results of the 3 groups of the conventional-sequence DNA nanoparticles are shown below in Table 37 to 39: 
     
       
         
           
               
               
               
             
               
                 TABLE 37 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP  
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 D-2 
                 1 
                 −36.00 
               
               
                   
                 2 
                 −35.80 
               
               
                   
                 3 
                 −37.60 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −36.47 mV (±0.67 mV) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 38 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP  
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 D-6 
                 1 
                 −31.70 
               
               
                   
                 2 
                 −32.10 
               
               
                   
                 3 
                 −31.90 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −31.90 mV (±0.2 mV) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
             
               
                 TABLE 39 
               
               
                   
               
               
                 Sample 
                 Detection 
                 Potential ZP  
               
               
                 name 
                 times 
                 (mV) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 D-7 
                 1 
                 −32.10 
               
               
                   
                 2 
                 −31.70 
               
               
                   
                 3 
                 −32.80 
               
            
           
           
               
               
            
               
                 Experiment result 
                 −32.20 mV (±0.0.5 mV) 
               
               
                   
               
            
           
         
       
     
     It may be seen from the above potential detection data that the 3 groups of the conventional-sequence RNA self-assembly products have good stability, it is further indicated that the nanoparticles formed by the self-assembly of each conventional-sequence RNA have a relatively stable set-assembly structure. 
     (3) Particle Size Measurement 
     1. Preparing a potential sample (conventional-sequence DNA self-assembly product D-7) and putting into a sample pool, opening a sample pool cover of an instrument, and placing the instrument; 
     2. opening software, and clicking a menu, so a manual measurement parameter setting dialog box appears; 
     3. setting a software detection parameter; and then 
     4. clicking Ok to complete the settings, when a measurement dialog box appears, clicking Start to start, a DLS measurement value result of a hydrodynamic size of the self-assembly product D-7 is shown below in Table 40: 
     
       
         
           
               
               
               
             
               
                 TABLE 40 
               
               
                   
               
               
                 Sample  
                 Detection 
                 Particle size 
               
               
                 name 
                 times 
                 Z-Ave (d.nm) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 1 
                 10.76 
               
               
                   
                 2 
                 13.9 
               
               
                   
                 3 
                 10.36 
               
            
           
           
               
               
            
               
                 Experiment result 
                 12.33 d.nm (±1.57 d.nm) 
               
               
                   
               
            
           
         
       
     
     The above conventional-sequence DNA self-assembly product D-7 is irradiated by a transmission electron microscope, and steps are as follows: 
     1. taking a drop of a sample and suspending on 400 meshes of a carbon-coated film copper grid, keeping for 1 min at a room temperature; 
     2. absorbing liquid by filter paper; 
     3. staining for 1 min by 2% uranyl acetate; 
     4. absorbing by the filter paper, and drying at the room temperature; and 
     5. observing and taking a picture by a transmission electron microscope JEM-1400 at 120 kv. 
     The result is as shown in  FIG. 9 , it may be apparently seen from the figure that the above conventional-sequence DNA self-assembly product D-7 is an integral structure, and it may be clearly seen that it has a T-type structure. 
     Embodiment 5 
     Tacrine Loading Experiment 
     Chemical Loading: 
     I. Experiment Material and Experiment Method 
     1. Experiment Materials and Reagents: 
     (1) Nucleic acid nanoparticles: RNA nanoparticles from Embodiment 1. 
     (2) DEPC treated water: Biyuntian Company. 
     (3) PBS buffer solution: cellgro. 
     (4) 4% paraformaldehyde 
     (5) Tacrine. 
     (6) Chloroform: Beijing Beihua Fine Chemicals Co., Ltd. 
     (7) Anhydrous ethanol: Beijing Beihua Fine Chemicals Co., Ltd. 
     2. Experiment Method: 
     (1) Accurately weighing the tacrine (0.32 ng, 1.354 μmol) and dissolving in the DEPC water (1.0 mL) and the PBS buffer solution (1.25 mL), adding 4% paraformaldehyde aqueous solution (0.4 mL) under cooling of an ice-water bath and uniformly mixing, enabling the mixed solution to be totally uniformly mixed with the RNA nanoparticles (I mg, 33.84 nmol), and reacting for 72 hours at 4 DEG C. under a dark condition. 
     (2) Taking 10 μL of reaction solution and diluting for 10 times, using 50 μM of the tacrine aqueous solution and 310 ng/μL of the RNA nanoparticles as controls, and performing HPLC analysis according to equal volume injection. According to a peak area ratio of each component, it may be adjusted that reaction conversion is basically completed. 
     (3) Extracting the reaction solution by the chloroform (10 mL×3), and then adding 25 ml of the anhydrous ethanol, after uniformly mixing, keeping at 4 DEG C. in the dark and enabling a product to be adequately precipitated (4 hours). Centrifuging (40001 min), transferring supernatant, and washing the solid product again by the ethanol (50 mL), evaporating a solvent under reduced pressure and lower temperature, to obtain a loading product. 
     (4) Loading rate calculation: 
     1. preparing a known concentration of tacrine-HCl standard solution (the HCl concentration is 0.1 M): 2 μM, 4 μM, 6 μM, 8 μM, and 10 μM, 100 μL each; 
     2. enabling tacrine-RNAh particles to be dissolved in 100 μL of the PBS; 
     3. putting the standard solution and the tacrine-RNAh particles into a PCR plate, heating at 85 DEG C. for 5 min, and then cooling to the room temperature; 
     4. measuring an absorbance of the tacrine at 240 nm by using a microplate reader, drawing a standard curve (as shown in  FIG. 10 ), and calculating the molar concentration of the tacrine in the loaded product; 
     5. measuring an absorbance of RNA at 260 nm by using a spectrophotometer, to obtain the mass concentration of the tacrine-RNAh particles contained in each sample; and 
     6. according to the tacrine molar concentration and the mass concentration of the RNAh particles obtained by measuring, calculating a loading rate. 
     A specific calculation process is as follows: 
     
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   1 
                 
               
               = 
               
                 76.0 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   μ 
                 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   t 
                   
                     acrine 
                     - 
                     1 
                   
                 
               
               = 
               
                 42.24 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   2 
                 
               
               = 
               
                 52.0 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   μ 
                 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 
                   C 
                   
                     tacrine 
                     - 
                     1 
                   
                 
                 = 
                 
                   24.0 
                   ⁢ 
                   
                       
                   
                   ⁢ 
                   μ 
                   ⁢ 
                   
                       
                   
                   ⁢ 
                   M 
                 
               
               , 
               
                 
                   
                     100 
                     
                         
                     
                   
                   ⁢ 
                   μ 
                   ⁢ 
                   
                       
                   
                   ⁢ 
                   L 
                 
                 ; 
               
             
             ⁢ 
             
                 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 1 
               
               = 
               
                 
                   
                     42.24 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0760 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 16.7 
               
             
             ; 
           
         
       
       
         
           
             
               N 
               ⁢ 
               
                 - 
               
               ⁢ 
               2 
             
             = 
             
               
                 
                   24.0 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                 
                 
                   0.0520 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   
                     6 
                     / 
                     30000 
                   
                 
               
               = 
               
                 13.8 
                 . 
               
             
           
         
       
     
     An average value of N-1 and N-2 is taken so that the loading rate of RNAh-tacrine is about 16, and it means that about 15 tacrine molecules may be loaded on each nucleic acid nanoparticle carrier. 
     In addition, on the basis of the tacrine-loaded RNA nanoparticles, other small molecular drugs may be further loaded for the second time in the same way as the tacrine loading. For example, the present application is further loaded with a folic acid to obtain the RNA nanoparticles co-loaded with two small molecular drugs of the tacrine and the folic acid, and the loading rates of the two drugs may be detected by referring to the above method (values are not shown). 
     It is indicated from Embodiment 5 that the RNA nanoparticles (in Embodiment 1) with the extension fragment, the target head and the fluorescein have a function of loaded drugs, may achieve the loading with the small molecular drug tacrine in a mode of covalent linkage (paraformaldehyde-solvent covalence), and may also achieve the co-loading with other small molecular drugs. 
     Embodiment 6 
     Cell Binding Ability of Drug-Loaded RNA Nanoparticles Detected by Confocal Microscopy Experiment 
     I. Experiment Material and Experiment Method: 
     1. Samples to be tested are as shown in Table 41: 
     
       
         
           
               
               
               
             
               
                 TABLE 41 
               
               
                   
               
               
                 Nanoparticles 
                 MW 
                 Dissolution solvent 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 1 
                 RNAh-Biotin-quasar670 
                 29552.6 
                 PBS 
               
               
                 2 
                 RNAh-Biotin-quasar670-tacrine 
                 36591.738 
                 PBS 
               
               
                   
               
            
           
         
       
     
     Note: the RNAh-Biotine-quasar670 in the table is served as a control, and refers to the nanoparticle formed by performing the biotin modification at the 5′-end of the a-strand and b-strand prepared according to the self-assembly method in Embodiment 1, and performing the quasar670 fluorescein modification at the 3′-end of the c-strand, and the RNAh-Biotin-quasar670-tacrine refers to the nanoparticle formed after further loading the tacrine (loaded according to the chemical method in Embodiment 5). 
     2. Experiment reagents used and sources thereof are as follows: 
     RPMI-1640 medium (Gibco, C11875500BT-500 mL); DMEM (Gibco, C11995500BT-500 mL); Fetal bovine serum (FBS) (ExCell Bio, FNA500-500 mL); Penicillin/Streptomycin (PS) (Gibco, 15140-122-100 mL); PBS buffer solution (Gibco, C20012500BT-500 mL); Trypsin-EDTA (Stemcell, 07901-500 mL); DMSO (Sigma, D5879-1L); Prolong Gold Antifade Mountant (Thermo. P36941-2 mL); and DAPI (Yeasen, 36308ES11-4 mL). 
     3. Experiment instruments used are as follows: 
     Inverted Microscope (Olympus BX53, U-RFL-T); BD Falcon (Corning, 354118); and Cytospin (TXD3). 
     4. Experiment method: 
     (1) Culturing SH-SY5Y cells (neuroblastoma cell fine) in a RPMI1640+10% FBS+1% PS medium under a condition of 37 DEG C. and 5% CO 2 . 
     (2) Trypsin-digesting the SH-SY5Y cells, washing once with the PBS, and adding to a cell culture glass slide in 1×10 3  cells per well. 
     (3) After the cells are adhered to a wall, rinsing the glass slide with the culture medium. 
     (4) Incubating the cells with 200 nM and 400 nM of the RNAh-Biotin-quasar670 and RNAh-Biotin-quasar670-tacrine nanoparticles under 37 DEG C. and 5% CO 2  for 1 h and 4 hrs. 
     (5) After the adherent cells are washed with the PBS, treating with the Prolong Gold Antifade Mountant and keeping overnight at a room temperature. 
     (6) Staining with the DAPI for 5 min at the room temperature, and then sealing the glass side. 
     (7) Taking pictures under the microscope and saving. 
     II. Experiment Result 
     An experiment result is shown in  FIG. 11 . It may be seen from  FIG. 11  that the results of cell binding and internalization experiments show that the RNAh-Biotin-quasar670 and RNAh-Biotin-quasar670-tacrine nanoparticles may be both bound and internalized with the cells because they both carry the target head—Biotin. This result shows that the drug RNAh-Biotin-quasar670-tacrine nanoparticles containing the tacrine have a strong ability to bind and internalize with the SH-SY5Y cells. 
     Embodiment 7 
     Stability Detection of Tacrine-Containing Drug Loaded on Nucleic Acid Nanoparticles in Serum 
     I. Experiment Material and Experiment Method 
     1. Samples to be tested: RNAh-Biotin-quasar670-tacrine nanoparticles prepared in Embodiment 5 dissolved in PBS solution. 
     2. Experiment reagents: 
     RPMI-1640 medium (Gibco, C11875500BT-500 mL); Fetal bovine serum (FBS) (ExCell Bio, FNA500-500 mL); Penicillin/Streptomycin (PS) (Gibco, 15140-122-100 mL); PBS buffer solution (Gibco, C20012500BT-500 ml); Novex™ Tris-Glycine Native Sample Buffer (2×) (Invitrogen, LC2673-20 mL); Novex™ 8% Tris-Glycine Mini Gels (Invitrogen, XP00080BOX-1.0 mm); Tris-Glycine Native Running buffer (10×) (Life science, LC2672-500 mL); and G250 staining solution (Beyotime, P0017-250 mL). 
     3. Experiment instrument 
     Spectrophotometer (Thermo, ND2000C); Mini Gel Tank (Invitrogen, PS0301); and Imaging System (Bio-Rad, ChemiDoc MP). 
     4. Experiment method: 
     (1) Enabling the RNAh-Biotin-quasar670-tacrine nanoparticles to be prepared to 100 μM, and adequately mixing uniformly. 
     (2) Taking 1 μL of solution and placing in 99 μL of a RPMI 1640 medium containing 10% serum and incubating. 
     (3) After being incubated at 37 DEG C. for 10 min, 1 h, 12 h, and 36 h, respectively taking samples. 
     (4) After using NanoDrop for quantification, taking 200 ng of the RNA nanoparticles, adding the same volume of Tris-Glycine SDS sample buffer solution (2×), and adequately mixing uniformly. 
     (5) Taking a piece of Novex™ 8% Tris-Glycine Mini gel, loading the samples in order, setting a program at 200 V, 30 min, and starting electrophoresis. 
     (6) After the electrophoresis is finished, performing G250 staining, placing on a horizontal shaker for 30 min-1 h, taking pictures and imaging. 
     II. Experiment Result 
     
       
         
           
               
             
               
                 TABLE 42 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 200 ng 
                 Buffer 
               
               
                   
                 RNAh-Biotin-quasar670-tacrine 
                 RNAh−Biotin−quasar670−tacrine 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 95.2 
                 2.10 
                 2.10 
               
               
                 10 
                 min 
                 96.0 
                 2.08 
                 2.08 
               
               
                 1  
                 h 
                 95.3 
                 2.10 
                 2.10 
               
               
                 12 
                 h 
                 96.0 
                 2.08 
                 2.08 
               
               
                 36  
                 h 
                 124.8 
                 1.60 
                 1.60 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 12  and  FIG. 13 . Herein,  FIG. 12  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 13  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that 0 min, 10 min, 1 h, 12 h and 36 h, under different time lengths, there is no significant difference between the bands of RNAh-Biotin-quasar670-tacrine nanoparticles, it is indicated that RNAh-Biotin-quasar670-tacrine nanoparticles are relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     Embodiment 8 
     Cytotoxicity research of RNAh-Biotin-quasar670tacrine nanoparticles in SH-SY5Y cells 
     I. Experiment Material and Experiment Method 
     1. Samples to be tested are a DMSO control, a small molecular drug tacrine and RNAh-Biotin-quasar670-tacrine nanoparticles. 
     2. Experiment reagent 
     RPMI-1640 medium (Gibco, C11875500BT-500 mL); DMEM (Gibco, C11995500BT-500 mL); Fetal bovine serum (FBS) (ExCell Bio, FNA500-500 mL); Penicillin/Streptomycin (Penicillin/Streptomycin, PS) (Gibco, 15140-122-100 mL); PBS buffer solution (Gibco, C20012500BT-500 mL); Trypsin-EDTA (Stemcell, 07901-500 ml); DMSO (Sigma, D5879-1 L); Dox (HISUN Pharm, H33021980-10 mg): and Cell Titer-Glo Luminescent Cell Viability Assay kit (CTG) (Promega, G7572-100 mL). 
     3. Experiment instrument: 
     Inverted Microscope (Olympus IX71, TH4-200); and 96-well Plate Reader (Molecular Devices, Flexstation 3). 
     4. Experiment method: 
     (1) Using the RPMI1640+10% FBS+1% PS medium to culture the SH-SY5Y cells at 37 DEG C and 5% CO 2 . 
     (2) Collecting the cells, centrifuging at 800 rpm for 5 minutes, resuspending the medium, adjusting the cell concentration, and adding to the 96-well plate in a volume of 5000 cells per 90 μL. 
     (3) Diluting a sample to be tested with the culture medium on the next day, respectively adding 200 nM to each sample, herein each sample has 4 replicate wells for replication. 
     (4) After being cultured for 72 hours, adding 100 μL of the CTG reagent to each well, shaking for 2 minutes, and standing at the room temperature for 10 minutes, herein a whole process is protected from light. 
     (5) Finally using Soft Max Pro5 software to read. 
     II. Experiment Result 
     
       
         
           
               
             
               
                 TABLE 43 
               
             
            
               
                   
               
               
                 cell proliferation rate (%) 
               
            
           
           
               
               
               
               
            
               
                 Cell  
                 Treatment 
                   
                   
               
               
                 line 
                 time 
                 Tacrine 
                 RNAh-Biotin-quasar670-tacrine 
               
               
                   
               
               
                 SH-SY5Y 
                 72 h 
                 39.75 
                 13.04 
               
               
                   
               
            
           
         
       
     
     The experiment results are shown in Table 43 and  FIG. 14 . It may be seen from Table 43 and  FIG. 14  that 200 nM of the RNA nanoparticle RNAh-Biotin-quasar670-tacrine carrying the tacrine has the apparent cytotoxicity to the SH-SY5Y cells (P&lt;0.0001), and it is unpredictable that: compared with the small molecular drug tacrine on cell proliferation inhibition, 200 nM of the RNAh-Biotin-quasar670-tacrine has the more significant inhibition to the proliferation of the SH-SY5Y cells, and the call proliferation rate is further reduced by at least 213 (to 13.04%) on the basis of 39.75% of the proliferation rate of the cells after being treated with the small molecular drug tacrine. 
     In order to further determine that the RNA nanoparticles without carrying the tacrine have no apparent cytotoxicity to the SH-SY5Y cells, the inventor further designs a toxicity experiment of a targeted fluorescent carrier RNAh-Biotin-Cy5 to SH-SY5Y cells, and uses another small molecular drug Cisplatin as a control (the highest administration concentration of the drug in the experiment is 5 μM). The result thereof are shown in Table 44 and  FIG. 15 . It can be seen from an IC 50  value in Table 44 and  FIG. 15  that the targeted fluorescent carrier without carrying the tacrine has no apparent toxicity to the experimental cells. 
     
       
         
           
               
               
               
             
               
                 TABLE 44 
               
               
                   
               
               
                   
                 Bio-Cy5-RNAh 
                 Cisplatin 
               
               
                   
               
             
            
               
                 IC50 (μM) 
                 &gt;5 
                 0.51 
               
               
                   
               
            
           
         
       
     
     Assembly of Nucleic Acid Nanoparticles 
     Embodiment 9 
     I. 7 Groups of Extension Fragment Deformation+Core Short-Sequence RNA Nanoparticle Carriers: 
     (1) 7 groups of three polynucleoside base sequences for forming extension fragment deformation+core short-sequence RNA nanoparticles: 
     
       
         
           
               
             
               
                 TABLE 45 
               
             
            
               
                   
               
               
                 R-15 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 120) 
                 GCGGCGAGCGGCGA GGAGCGU   UGG GGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio); bases C and U, and 2′F modification 
                 12668.8 
                 33713 
               
               
                   
               
               
                 b (SEQ ID NO: 121) 
                 CCGGCCUCCGGCC   CCUUCGGG       G   CCAGCCGCC 
                 31 
                 bases C and U, and 2′F modification 
                 9866.8 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 122) 
                 GGCGGCAGG   CCCCCAUAGCCC    UCGCCGCUCGCCGC 
                 35 
                 bases C and U, and 2′F modification 
                 11177.4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 46 
               
             
            
               
                   
               
               
                 R-16: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 123) 
                 GCGGCGAGCGGCGAGCAGCGU UCGGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio); bases C and U, and 2′F modification 
                 12591.6 
                 33709.8 
               
               
                   
               
               
                 b (SEQ ID NO: 124) 
                 CCGGCCUCCGGCCCGUUCGCC GCCAGCCGCC 
                 31 
                 bases C and U, and 2′F modification 
                 9827.6 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 125) 
                 GGCGGCAGGCGGCCAUAGCGC UCGCCGCUCGCCGC 
                 35 
                 bases C and U, and 2′F modification 
                 11290.6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 47 
               
             
            
               
                   
               
               
                 R-17: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 126) 
                 GCGGCGAGCGGCGA   GGAGCGU       UGG   GGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio); bases C and U, and 2′F modification 
                 12668.9 
                 33713 
               
               
                   
               
               
                 b (SEQ ID NO: 127) 
                 CCGGCCUCCGGCC   CCUUCGCC       G   CCAGCCGCC 
                 31 
                 bases C and U, and 2’F modification 
                 9790.6 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 128) 
                 GGCGGCAGG   CGGCCAUAGCCC    UCGCCGCUCGCCGC 
                 35 
                 bases C and U, and 2’F modification 
                 11253.5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 48 
               
             
            
               
                   
               
               
                 R-18: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 129) 
                 GCGGCGAGCGGCGAGCAGCGU UCGGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio); bases C and U, and 2′F modification 
                 12591.6 
                 33709.8 
               
               
                   
               
               
                 b (SEQ ID NO: 130) 
                 CCGGCCUCCGGCCCGUUCGGC GCCAGCCGCC 
                 31 
                 bases C and U, and 2′F modification 
                 9865.7 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 131) 
                 GGCGGCAGGCGCCCAUAGCGC UCGCCGCUCGCCGC 
                 35 
                 bases C and U, and 2′F modification 
                 11252.5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 49 
               
             
            
               
                   
               
               
                 R-19: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 132) 
                 GCGGCGAGCGGCGAGCAGCGU UCGGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio); bases C and U, and 2′F modification 
                 12591.6 
                 33709.8 
               
               
                   
               
               
                 b (SEQ ID NO: 133) 
                 CCGGCCUCCGGCCCGUUCGGC CCCAGCCGCC 
                 31 
                 bases C and U, and 2′F modification 
                 9827.6 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 134) 
                 GGCGGCAGGGGCCCAUAGCGC UCGCCGCUCGCCGC 
                 35 
                 bases C and U, and 2′F modification 
                 11290.6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 50 
               
             
            
               
                   
               
               
                 R-20: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 135) 
                 GCGGCGAGCGGCGACGAGCGU UGCGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio); bases C and U, and 2′F modification 
                 12591.6 
                 33709.8 
               
               
                   
               
               
                 b (SEQ ID NO: 136) 
                 CCGGCCUCCGGCCGCUUCGCC GCCAGCCGCC 
                 31 
                 bases C and U, and 2′F modification 
                 9827.6 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 137) 
                 GGCGGCAGGCGGCCAUAGCCG UCGCCGCUCGCCGC 
                 35 
                 bases C and U, and 2′F modification 
                 11290.6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 51 
               
             
            
               
                   
               
               
                 R-21: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 138) 
                 GCGGCGAGCGGCGACGAGCGU UGCGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio); bases C and U, and 2′F modification 
                 11290.6 
                 32408.8 
               
               
                   
               
               
                 b (SEQ ID NO: 139) 
                 CCGGCCUCCGGCCGCUUCGGC GCCAGCCGCC 
                 31 
                 bases C and U, and 2′F modification 
                 9865.7 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 140) 
                 GGCGGCAGGCGCCCAUAGCCG UCGCCGCUCGCCGC 
                 35 
                 bases C and U, and 2′F modification 
                 11252.5 
               
               
                   
               
            
           
         
       
     
     II. Self-Assembly Experiment Steps: 
     (1) according to a molar ratio of 1:1:1, enabling the RNA single strands a, b and c to be simultaneously mixed and dissolved in DEPC treated water or TMS buffer solution, 
     (2) heating nixed solution to 80 DEG C. after keeping for 5 m, slowly cooling to a room temperature at a rate of 2 DEG C./min; 
     (3) enabling a product to be loaded on 8% (m/v) of non-denaturing PAGE gel and placed in TBM buffer solution under a condition of 4 DEG C., purifying a complex by 100 v of electrophoresis; 
     (4) cutting a target bond and eluting in RNA elution buffer solution at 37 DEG C. after that, precipitating overnight in ethanol, and volatilizing under reduced pressure and low temperature, and 
     (5) electrophoresis analysis detection and laser scanning observation. 
     III. Self-Assembly Experiment Result 
     (1) Electrophoresis Detection 
     Main reagents and instruments are as follows: 
     
       
         
           
               
               
               
             
               
                   
               
               
                 Reagent name 
                 Article number 
                 Manufacturer 
               
               
                   
               
             
            
               
                 6 × DNA Loading buffer 
                 TSJ010 
                 Qingke Biotechnology Co., Ltd. 
               
               
                 20 bp DNA Ladder 
                 3420A 
                 TAKARA 
               
               
                 10000*SolarGelRed nucleic acid dye 
                 E1020 
                 solarbio 
               
               
                 8% non-denaturing PAGE gel 
                 / 
                 Self-made 
               
               
                 1 × TBE Buffer (RNA enzyme-free) 
                 / 
                 Self-made 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 53 
               
               
                   
                   
               
               
                   
                 Name 
                 Model 
                 Manufacturer 
               
               
                   
                   
               
             
            
               
                   
                 Electrophoresis apparatus 
                 PowerPac Basic 
                 Bio-rad 
               
               
                   
                 Vertical electrophoresis cell 
                 Mini PROTEAN 
                 Bio-rad 
               
               
                   
                   
                 Tetra Cell 
                   
               
               
                   
                 Discoloration shaker 
                 TS-3D 
                 orbital shaker 
               
               
                   
                 Gel imager 
                 Tanon 3500 
                 Tanon 
               
               
                   
                   
               
            
           
         
       
     
     Steps: 
     {circle around (1)} The RNA nanoparticles are diluted with ultrapure water by using a method shown below in Table 54. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 54 
               
               
                   
                   
               
               
                   
                   
                 Measured concentration 
               
               
                   
                 Serial number 
                 (μg/mL) 
               
               
                   
                   
               
             
            
               
                   
                 R-15 
                 165.937 
               
               
                   
                 R-16 
                 131.706 
               
               
                   
                 R-17 
                 144.649 
               
               
                   
                 R-18 
                 164.743 
               
               
                   
                 R-19 
                 126.377 
               
               
                   
                 R-20 
                 172.686 
               
               
                   
                 R-21 
                 169.455 
               
               
                   
                   
               
            
           
         
       
     
     {circle around (2)} 10 μL (500 ng) of the processed sample is taken and uniformly mixed with 2 μL of 6×DNA Loading Buffer, it is operated on ice, and a label is made. 
     {circle around (3)} A 8% non-denaturing PAGE gel is taken, a piece of the gel is applied to samples with different incubation times, 12 μL of the processed samples are all loaded, and a program is set to run the gel at 100V for 40 minutes. 
     {circle around (4)} After running the gel, dyeing is performed, it is placed on a horizontal shaker for 30 minutes, and pictures are taken for Imaging. 
     Detection Result: 
     Non-denaturing PAGE gel running results of the 7 groups of extension fragment deformation+core short-sequence RNA self-assembly products are shown in  FIG. 16 . Lanes 1 to 7 in  FIG. 16  from left to right are successively: the 7 groups of extension fragment deformation+core short-sequence RNA self-assembly products R-15, R-16, R-17, R-18, R-19, R-20 and R-21. 
     It may be clearly seen from the results in  FIG. 16  that the bonds of the 7 groups of extension fragment deformation+core short-sequence RNA self-assembly products are bright and clear, it is indicated that the 7 groups of extension fragment deformation+core short-sequence RNA strands are all set-assembled completely, to form a stable nanoparticle structure. 
     (2) Electric Potential Measurement 
     Measurement method: preparing a potential sample (the self-assembly product is dissolved in ultrapure water) and putting into a sample pool, opening a sample pool cover of an instrument, and placing the instrument: 
     opening software, clicking a menu MeasUre€ManUal, so a manual measurement parameter setting dialog box appears; 
     setting a software detection parameter; 
     and then clicking Ok to complete the settings, after a measurement dialog box appears, clicking Start to start. 
     Measurement result potential detection results of the 7 groups of the extension fragment deformation+core short-sequence RNA nanoparticles at 25 DEG C. are as follows: 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 55 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-15 
                 1 
                 −22.50 
               
               
                   
                   
                 2 
                 −23.90 
               
               
                   
                   
                 3 
                 −23.30 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −23.23 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 56 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-16 
                 1 
                 −21 10 
               
               
                   
                   
                 2 
                 −19.80 
               
               
                   
                   
                 3 
                 −21.90 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −20.93 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 57 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-17 
                 1 
                 −24.90 
               
               
                   
                   
                 2 
                 −20.90 
               
               
                   
                   
                 3 
                 −24.70 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −23.50 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 58 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-18 
                 1 
                 −20.80 
               
               
                   
                   
                 2 
                 −21.30 
               
               
                   
                   
                 3 
                 −21.70 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −21.27 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 59 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-19 
                 1 
                 −16.80 
               
               
                   
                   
                 2 
                 −16.90 
               
               
                   
                   
                 3 
                 −21.20 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −18.30 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 60 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-20 
                 1 
                 −16.00 
               
               
                   
                   
                 2 
                 −16.90 
               
               
                   
                   
                 3 
                 −21.20 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −17.90 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 61 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 R-21 
                 1 
                 −15.20 
               
               
                   
                   
                 2 
                 −16.70 
               
               
                   
                   
                 3 
                 −16.40 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −16.10 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     It may be seen from the above potential detection data that: the 7 groups of extension fragment deformation+core short-sequence RNA nanoparticles all have the good stability, and it is further indicated that the nanoparticles formed by the self-assembly of each extension fragment deformation+core short-sequence RNA have a relative stable self-assembly structure. 
     (3) Particle Size Measurement 
     1. Preparing a potential sample (7 groups of extension fragment deformation+core short-sequence RNA) and putting into a sample pool, opening a sample pool cover of an instrument, and placing the instrument; 
     2. opening software, and clicking a menu, so a manual measurement parameter setting dialog box appears; 
     3. setting a software detection parameter; and then 
     4. clicking Ok to complete the settings, when a measurement dialog box appears, clicking Start to start, a DLS measurement value result of a hydrodynamic size of the 7 groups of extension fragment deformation+core short-sequence RNA is as follows: 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 62 
               
               
                   
                   
               
               
                   
                   
                 Mean particle 
               
               
                   
                 Serial number 
                 size (nm) 
               
               
                   
                   
               
             
            
               
                   
                 R-15 
                 6.808 
               
               
                   
                 R-16 
                 6.978 
               
               
                   
                 R-17 
                 7.592 
               
               
                   
                 R-18 
                 7.520 
               
               
                   
                 R-19 
                 6.936 
               
               
                   
                 R-20 
                 7,110 
               
               
                   
                 R-21 
                 6.720 
               
               
                   
                   
               
            
           
         
       
     
     (4) TM Value Detection 
     A solubility curve method is used to detect TM values of the 7 groups of the extension fragment deformation+core short-sequence RNA nanoparticles, and the samples are consistent with the potential samples. 
     Reagents and instruments are as follows: 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 63 
               
               
                   
                   
               
               
                   
                 Reagent name 
                 Article number 
                 Manufacturer 
               
               
                   
                   
               
             
            
               
                   
                 AE buffer 
                 / 
                 Takara 
               
               
                   
                 SYBR Green I Dye 
                 / 
                 Self-made 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 64 
               
               
                   
                   
               
               
                   
                 Name 
                 Model 
                 Manufacturer 
               
               
                   
                   
               
             
            
               
                   
                 Real-Time System 
                 CFX Connect 
                 Bio-rad 
               
               
                   
                 Clean bench 
                 HDL 
                 Beijing Donglian Haer 
               
               
                   
                   
                   
                 Instrument Manufacturing 
               
               
                   
                   
                   
                 Co., Ltd. 
               
               
                   
                   
               
            
           
         
       
     
     Steps: 
     {circle around (1)} After the sample is diluted with ultrapure water, 5 μg of the diluted sample is mixed with 2 μL of SYBR Green I dye (diluted by 1:200), a final volume is 20 μL, and the dilution concentration is as follows: 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 65 
               
               
                   
                   
               
               
                   
                   
                 Measured concentration 
               
               
                   
                 Serial number 
                 (μg/mL) 
               
               
                   
                   
               
             
            
               
                   
                 R-15 
                 773.009 
               
               
                   
                 R-16 
                 782.098 
               
               
                   
                 R-17 
                 740.607 
               
               
                   
                 R-18 
                 806.163 
               
               
                   
                 R-19 
                 829.996 
               
               
                   
                 R-20 
                 723.082 
               
               
                   
                 R-21 
                 721.674 
               
               
                   
                   
               
            
           
         
       
     
     {circle around (2)} It is incubated for 30 min in the dark at a room temperature; and 
     {circle around (3)} On-machine detection is performed, a program is set to start at 20 DEG C., the temperature rises per second from 0.1 DEG C. to 95 DEG C., and reading is performed every 5 s. 
     Detection Result: 
     The TM values of the 7 groups of extension fragment deformation+core short-sequence RNA nanoparticles are as follows, a solubility curve diagram of the R-15 is shown in  FIG. 17 , a solubility curve diagram of the R-16 is shown in  FIG. 18 , a solubility curve diagram of the R-17 is shown in  FIG. 19 , a solubility curve diagram of the R-18 is shown in  FIG. 20 , a solubility curve diagram of the R-19 is shown in  FIG. 21 , a solubility curve diagram of the R-20 is shown in  FIG. 22 , and a solubility curve diagram of the R-21 is shown in  FIG. 23 . Due to the particularity of the RNA samples, a temperature corresponding to ½ RFUmax in a range of 20 to 90 DEG C. is used as a sample Tm value in this test. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 66 
               
               
                   
                   
               
               
                   
                   
                 TM value (° C.) 
               
               
                   
                   
               
             
            
               
                   
                 R-15 
                 57.5° C. 
               
               
                   
                 R-16 
                 53.8° C. 
               
               
                   
                 R-17 
                 55.2° C. 
               
               
                   
                 R-18 
                 54.5° C. 
               
               
                   
                 R-19 
                 54.0° C. 
               
               
                   
                 R-20 
                 59.5° C. 
               
               
                   
                 R-21 
                 65.0° C. 
               
               
                   
                   
               
            
           
         
       
     
     The TM values of the 7 groups of extension fragment deformation+core short-sequence RNA nanoparticles are all high, it is indicated that the self-assembly products have the good structural stability. 
     Embodiment 10 
     I. 7 Groups of Extension Fragment Deformation+Core Short-Sequence DNA Nanoparticle Carriers: 
     (1) 7 groups of three polynucleotide base sequences for forming extension fragment deformation+core short-sequence DNA nanoparticles: 
     
       
         
           
               
             
               
                 TABLE 67 
               
             
            
               
                   
               
               
                 D-8: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 141) 
                 GCGGCGAGCGGCGA   GGAGCGT       TGG   GGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio) 
                 12087.3 
                 32081.8 
               
               
                   
               
               
                 b (SEQ ID NO: 142) 
                 CCGGCCTCCGGCC   CCTTCGGG       G   CCAGCCGCC 
                 31 
                   
                 9375.1 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 143) 
                 GGCGGCAGG   CCCCCATAGCCC    TCGCCGCTCGCCGC 
                 35 
                   
                 10619.4 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 68 
               
             
            
               
                   
               
               
                 D-9: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 144) 
                 GCGGCGAGCGGCGAGCAGCGT TCGGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio) 
                 12000.4 
                 32071.6 
               
               
                   
               
               
                 b (SEQ ID NO: 145) 
                 CCGGCCTCCGGCCCGTTCGCC GCCAGCCGCC 
                 31 
                   
                 9333.2 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 146) 
                 GGCGGCAGGCGGCCATAGCGC TCGCCGCTCGCCGC 
                 35 
                   
                 10738 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 69 
               
             
            
               
                   
               
               
                 D-10: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 147) 
                 GCGGCGAGCGGCGA   GGAGCGT       TGG   GGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio) 
                 12087.7 
                 32081.6 
               
               
                   
               
               
                 b (SEQ ID NO: 148) 
                 CCGGCCTCCGGCC   CCTTCGCC       G   CCAGCCGCC 
                 31 
                   
                 9294.3 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 149) 
                 GGCGGCAGG   CGGCCATAGCCC    TCGCCGCTCGCCGC 
                 35 
                   
                 10699.6 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 70 
               
             
            
               
                   
               
               
                 D-11: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 150) 
                 GCGGCGAGCGGCGAGCAGCGT TCGGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio) 
                 12000.4 
                 32071.6 
               
               
                   
               
               
                 b (SEQ ID NO: 151) 
                 CCGGCCTCCGGCCCGTTCGGC GCCAGCCGCC 
                 31 
                   
                 9333.2 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 152) 
                 GGCGGCAGGCGCCCATAGCGC TCGCCGCTCGCCGC 
                 35 
                   
                 10738 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 71 
               
             
            
               
                   
               
               
                 D-12: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 153) 
                 GCGGCGAGCGGCGAGCAGCGT TCGGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio) 
                 12000.4 
                 32071.6 
               
               
                   
               
               
                 b (SEQ ID NO: 154) 
                 CCGGCCTCCGGCCCGTTCGGC CCCAGCCGCC 
                 31 
                   
                 9333.2 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 155) 
                 GGCGGCAGGGGCCCATAGCGC TCGCCGCTOGCCGC 
                 35 
                   
                 10738 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 72 
               
             
            
               
                   
               
               
                 D-13: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ ID NO: 156) 
                 GCGGCGAGCGGCGACGAGCGT TGCGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with a biotin (Bio) 
                 12000.4 
                 32071.6 
               
               
                   
               
               
                 b (SEQ ID NO: 157) 
                 CCGGCCTCCGGCCGCTTCGCC GCCAGCCGCC 
                 31 
                   
                 9333.2 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 158) 
                 GGCGGCAGGCGGCCATAGCCG TCGCCGCTCGCCGC 
                 35 
                   
                 10738 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 73 
               
             
            
               
                   
               
               
                 D-14: 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Total 
               
               
                   
                   
                 Base 
                 Chemical 
                 Molecular 
                 molecular 
               
               
                 Name 
                 Sequence sense (5′-3′) 
                 number 
                 modification 
                 weight 
                 weight 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 a (SEQ 
                 GCGGCGAGCGGCGACGAGCGT TGCGGCCGGAGGCCGG 
                 37 
                 3′-end is linked with 
                 12000.4 
                 32071.6 
               
               
                 ID NO: 159) 
                   
                   
                 a biotin (Bio) 
                   
                   
               
               
                   
               
               
                 b (SEQ ID NO: 160) 
                 CCGGCCTCCGGCCGCTTCGGC GCCAGCCGCC 
                 31 
                   
                 9373.2 
                   
               
               
                   
               
               
                 c (SEQ ID NO: 161) 
                 GGCGGCAGGCGCCCATAGCCG TCGCCGCTCGCCGC 
                 35 
                   
                 10698 
               
               
                   
               
            
           
         
       
     
     II. Self-Assembly Experiment Steps: 
     (1) according to a molar ratio of 1:1:1, enabling the DNA single strands a, b and c to be simultaneously mixed and dissolved in DEPC treated water or TMS buffer solution; 
     (2) heating mixed solution to 95 DEG C., after keeping for 5 min, slowly cooling to a room temperature at a rate of 2 DEG C./min; 
     (3) enabling a product to be loaded on 8% (m/v) of non-denaturing PAGE gel and placed in TBM buffer solution under a condition of 4 DEG C., purifying a complex by 100 v of electrophoresis; 
     (4) cutting a target band and eluting in DNA elution buffer solution at 37 DEG C., after that, precipitating overnight in ethanol, and volatilizing under reduced pressure and low temperature, to obtain the DNA self-assembly products; 
     (5) electrophoresis analysis detection and laser scanning observation; 
     (6) electric potential detection; 
     (7) particle size detection; and 
     (8) TM value detection. 
     III. Self-Assembly Experiment Result 
     (1) Electrophoresis Detection 
     Main reagents and instruments are as follows: 
     
       
         
           
               
               
               
             
               
                 TABLE 74 
               
               
                   
               
               
                 Reagent name 
                 Article number 
                 Manufacturer 
               
               
                   
               
             
            
               
                 6 × DNA Loading buffer 
                 TSJ010 
                 Qingke Biotechnology 
               
               
                   
                   
                 Co., Ltd 
               
               
                 20 bp DNA Ladder 
                 3420A 
                 TAKARA 
               
               
                 10000*SolarGelRed nucleic 
                 E1020 
                 solarbio 
               
               
                 acid dye 
                   
                   
               
               
                 8% non-denaturing PAGE gel 
                 / 
                 Self-made 
               
               
                 1 × TBE Buffer (RNA 
                 / 
                 Self-made 
               
               
                 enzyme-free) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 75 
               
               
                   
                   
               
               
                   
                 Name 
                 Model 
                 Manufacturer 
               
               
                   
                   
               
             
            
               
                   
                 Electrophoresis apparatus 
                 PowerMac Basic 
                 Bio-rad 
               
               
                   
                 Vertical electrophoresis cell 
                 Mini PROTEAN 
                 Bio-rad 
               
               
                   
                   
                 Tetra Cell 
                   
               
               
                   
                 Discoloration shaker 
                 TS-3D 
                 orbital shaker 
               
               
                   
                 Gel imager 
                 Tanon 3500 
                 Tanon 
               
               
                   
                   
               
            
           
         
       
     
     Steps: 
     {circle around (1)} The DNA nanoparticles are diluted with ultrapure water by using a method shown in Table 76 below. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 76 
               
               
                   
                   
               
               
                   
                   
                 Measured concentration 
               
               
                   
                   
                 (μg/mL) 
               
               
                   
                   
               
             
            
               
                   
                 D-8 
                 2890.932 
               
               
                   
                 D-9 
                 2238.682 
               
               
                   
                 D-10 
                 2075.084 
               
               
                   
                 D-11 
                 3117.389 
               
               
                   
                 D-12 
                 2880.939 
               
               
                   
                 D-13 
                 2704.757 
               
               
                   
                 D-14 
                 3216.917 
               
               
                   
                   
               
            
           
         
       
     
     {circle around (2)} 10 μL (500 ng) of the processed sample is taken and uniformly mixed with 2 μL of 6×DNA Loading Buffer, it is operated on ice, and a label is made. 
     {circle around (3)} A 8% non-denaturing PAGE gel is taken, a piece of the gel is applied to samples with different incubation times, 12 μL of the processed samples are all loaded, and a program is set to run the gel at 100V for 40 minutes. 
     {circle around (4)} After running the gel, dyeing is performed, it is placed on a horizontal shaker for 30 minutes, and pictures are taken for imaging. 
     Detection Result: 
     Non-denaturing PAGE gel running results of the 7 groups of extension fragment deformation+core short-sequence DNA self-assembly products are shown in  FIG. 24 . Lanes 1 to 7 in  FIG. 24  from left to right are successively: the 7 groups of extension fragment deformation+core short-sequence DNA self-assembly products D-8, D-9, D-10, D-11, D-12, D-13 and D-14. 
     It may be clearly seen from the results in  FIG. 24  that the bands of the 7 groups of extension fragment deformation+core short-sequence DNA self-assembly products are bright and clear, it is indicated that the 7 groups of extension fragment deformation+core short-sequence DNA strands are all self-assembled completely, to form a stable nanoparticle structure. 
     (2) Electric Potential Measurement 
     Measurement method: preparing a potential sample (the self-assembly product is dissolved in ultrapure water) and putting into a sample pool, opening a sample pool cover of an instrument, and placing the instrument; 
     opening software, clicking a menu MeasUre€ManUal, so a manual measurement parameter setting dialog box appears; 
     setting a software detection parameter; and then 
     clicking Ok to complete the settings, after a measurement dialog box appears, clicking Start to start. 
     Measurement result potential detection results of the 7 groups of the extension fragment deformation+core short-sequence DNA nanoparticles at 25 DEG C. are as follows: 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 77 
               
               
                   
                   
               
               
                   
                   
                 Detection 
                   
               
               
                   
                 Sample name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 D-8 
                 1 
                 −29.20 
               
               
                   
                   
                 2 
                 −32.90 
               
               
                   
                   
                 3 
                 −28.60 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −30.23 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 78 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 D-9 
                 1 
                 −39.20 
               
               
                   
                   
                 2 
                 −34.20 
               
               
                   
                   
                 3 
                 −31.80 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −35.07 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 79 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 D-10 
                 1 
                 −27.90 
               
               
                   
                   
                 2 
                 −28.30 
               
               
                   
                   
                 3 
                 −21.10 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −25.77 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 80 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 D-11 
                 1 
                 −29.70 
               
               
                   
                   
                 2 
                 −24.80 
               
               
                   
                   
                 3 
                 −27.80 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −27.43 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 81 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 D-12 
                 1 
                 −33.50 
               
               
                   
                   
                 2 
                 −29.80 
               
               
                   
                   
                 3 
                 −34.20 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −32.50 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 82 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 D-13 
                 1 
                 −26.80 
               
               
                   
                   
                 2 
                 −27.80 
               
               
                   
                   
                 3 
                 −26.40 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −27.00 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 83 
               
               
                   
                   
               
               
                   
                 Sample 
                 Detection 
                   
               
               
                   
                 name 
                 times 
                 Potential ZP (mV) 
               
               
                   
                   
               
             
            
               
                   
                 D-14 
                 1 
                 −31.70 
               
               
                   
                   
                 2 
                 −32.10 
               
               
                   
                   
                 3 
                 −22.40 
               
            
           
           
               
               
               
            
               
                   
                 Experiment 
                 −28.73 mV 
               
               
                   
                 result 
               
               
                   
                   
               
            
           
         
       
     
     It may be seen from the above potential detection data that: the 7 groups of extension fragment deformation+core short-sequence DNA nanoparticles all have the good stability, and it is further indicated that the nanoparticles formed by the self-assembly of each extension fragment deformation+core short-sequence DNA have a relative stable self-assembly structure. 
     (3) Particle Size Measurement 
     1. Preparing a potential sample (7 groups of extension fragment deformation+core short-sequence DNA) and putting into a sample pool, opening a sample pool cover of an instrument, and placing the instrument; 
     2. opening software, and clicking a menu, so a manual measurement parameter setting dialog box appears; 
     3. setting a software detection parameter; and then 
     4. clicking Ok to complete the settings, when a measurement dialog box appears, clicking Start to start, a DLS measurement value result of a hydrodynamic size of the 7 groups of extension fragment deformation+core short-sequence DNA is as follows: 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 84 
               
               
                   
                   
               
               
                   
                 Serial number 
                 Mean particle size (nm) 
               
               
                   
                   
               
             
            
               
                   
                 D-8 
                 7.460 
               
               
                   
                 D-9 
                 7.920 
               
               
                   
                 D-10 
                 7.220 
               
               
                   
                 D-11 
                 7.472 
               
               
                   
                 D-12 
                 6.968 
               
               
                   
                 D-13 
                 7.012 
               
               
                   
                 D-14 
                 6.896 
               
               
                   
                   
               
            
           
         
       
     
     (4) TM Value Detection 
     A solubility curve method is used to detect TM values of the 7 groups of the extension fragment deformation+core short-sequence DNA nanoparticles, and the samples are consistent with the potential samples. 
     Reagents and instruments are as follows: 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 85 
               
               
                   
                   
               
               
                   
                 Reagent name 
                 Article number 
                 Manufacturer 
               
               
                   
                   
               
             
            
               
                   
                 AE buffer 
                 / 
                 Takara 
               
               
                   
                 SYBR Green I Dye 
                 / 
                 Self-made 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 86 
               
               
                   
                   
               
               
                   
                 Name 
                 Model 
                 Manufacturer 
               
               
                   
                   
               
             
            
               
                   
                 Real-Time System 
                 CFX Connect 
                 Bio-rad 
               
               
                   
                 Clean bench 
                 HDL 
                 Beijing Donglian Haer 
               
               
                   
                   
                   
                 Instrument Manufacturing 
               
               
                   
                   
                   
                 Co., Ltd. 
               
               
                   
                   
               
            
           
         
       
     
     Steps: 
     {circle around (1)} After the sample is diluted with ultrapure water, 5 μg of the diluted sample is mixed with 2 μL of SYBR Green I dye (diluted by 1:200). a final volume is 20 μL, and the dilution concentration is as follows: 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 87 
               
               
                   
                   
               
               
                   
                   
                 Measured concentration 
               
               
                   
                 Serial number 
                 (μg/mL) 
               
               
                   
                   
               
             
            
               
                   
                 D-8 
                 2890.932 
               
               
                   
                 D-9 
                 2238.682 
               
               
                   
                 D-10 
                 2075.084 
               
               
                   
                 D-11 
                 3117.389 
               
               
                   
                 D-12 
                 2880.939 
               
               
                   
                 D-13 
                 2704.757 
               
               
                   
                 D-14 
                 3216.917 
               
               
                   
                   
               
            
           
         
       
     
     {circle around (2)} It is incubated for 30 min in the dark at a room temperature; and 
     {circle around (3)} On-machine detection is performed, a program is set to start at 20 DEG C., the temperature rises per second from 0.1 DEG C. to 95 DEG C., and reading is performed every 5 s. 
     Detection Result: 
     The TM values of the 7 groups of extension fragment deformation+core short-sequence DNA nanoparticles are as follows, a solubility curve diagram of the D-8 is shown in  FIG. 25 , a solubility curve diagram of the D-9 is shown in  FIG. 26 , a solubility curve diagram of the D-10 is shown in  FIG. 27 , a solubility curve diagram of the D-11 is shown in  FIG. 28 , a solubility curve diagram of the D-12 is shown in  FIG. 29 , a solubility curve diagram of the D-13 is shown in  FIG. 30 , and a solubility curve diagram of the D-14 is shown in  FIG. 31 . 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 88 
               
               
                   
                   
               
               
                   
                 Serial number 
                 TM value (° C.) 
               
               
                   
                   
               
             
            
               
                   
                 D-8 
                 48.5 
               
               
                   
                 D-9 
                 52.5 
               
               
                   
                 D-10 
                 54.5~55.0 
               
               
                   
                 D-11 
                 48.7 
               
               
                   
                 D-12 
                 51.5 
               
               
                   
                 D-13 
                 51.0 
               
               
                   
                 D-14 
                 49.2 
               
               
                   
                   
               
            
           
         
       
     
     It may be seen from  FIG. 25 to 31  that the TM values of the 7 groups of extension fragment deformation+core short-sequence DNA nanoparticles are all high, it is indicated that the self-assembly products have the good structural stability. 
     Stability Detection of Nucleic Acid Nanoparticles in Serum 
     Embodiment 11 
     A non-denaturing PAGE method is used to characterize the stability of 7 groups of extension fragment deformation+core short-sequence RNA nanoparticles in serum. 
     Main reagents and instruments are as follows: 
     
       
         
           
               
               
               
             
               
                 TABLE 89 
               
               
                   
               
               
                 Reagent name 
                 Article number 
                 Manufacturer 
               
               
                   
               
             
            
               
                 6 × DNA Loading buffer 
                 TSJ010 
                 Qingke Biotechnology 
               
               
                   
                   
                 Co., Ltd 
               
               
                 20 bp DNA Ladder 
                 3420A 
                 TAKARA 
               
               
                 10000*SolarGelRed nucleic 
                 E1020 
                 solarbio 
               
               
                 acid dye 
                   
                   
               
               
                 8% non-denaturing PAGE gel 
                 / 
                 Self-made 
               
               
                 1 × TBE Buffer (RNA 
                 / 
                 Self-made 
               
               
                 enzyme-free) 
                   
                   
               
               
                 Serum (FBS) 
                 / 
                 Excel 
               
               
                 RPMI 1640 
                 / 
                 GBICO 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 90 
               
               
                   
               
               
                   
                 Name 
                 Model 
                 Manufacturer 
                   
               
               
                   
               
             
            
               
                   
                 Electrophoresis apparatus 
                 PowerPac Basic 
                 Bio-rad 
                   
               
               
                   
                 Vertical electrophoresis  
                 Mini PROTEAN 
                 Bio-rad 
                   
               
               
                   
                 cell 
                 Tetra Cell 
                   
                   
               
               
                   
                 Discoloration shaker 
                 TS-3D 
                 orbital shaker 
                   
               
               
                   
                 Gel imager 
                 GenoSens1880 
                 Shanghai Qinxiang 
                   
               
               
                   
                   
                   
                 Scientific Instrument 
                   
               
               
                   
                   
                   
                 Co., Ltd. 
               
               
                   
               
            
           
         
       
     
     Steps: 
     {circle around (1)} The RNA nanoparticles are prepared to the concentration in the table below and then the prepared sample is diluted according to a method in the table below. It is diluted by 5 tubes. After being diluted, the sample is placed in a water bath at 37 DEG C. for different times (0, 10 min, 1 h, 12 h, and 38) 
     
       
         
           
               
               
               
               
               
               
             
               
                 TABLE 91 
               
               
                   
               
               
                   
                 Sample 
                   
                   
                   
                 Diluted 
               
               
                 Sample 
                 concentration 
                 Sample 
                 50% FBS- 
                 1640/ 
                 concentration 
               
               
                 name 
                 (μg/mL) 
                 volume 
                 1640/μL 
                 μL 
                 (μg/mL) 
               
               
                   
               
             
            
               
                 R-15 
                 773.009 
                 1.3 
                 4 
                 14.7 
                 50 μg/mL 
               
               
                 R-16 
                 782.098 
                 1.3 
                 4 
                 14.7 
                 50 μg/mL 
               
               
                 R-17 
                 740.607 
                 1.4 
                 4 
                 14.6 
                 50 μg/mL 
               
               
                 R-18 
                 806.163 
                 1.2 
                 4 
                 14.8 
                 50 μg/mL 
               
               
                 R-19 
                 829.996 
                 1.2 
                 4 
                 14.8 
                 50 μg/mL 
               
               
                 R-20 
                 723.082 
                 1.4 
                 4 
                 14.6 
                 50 μg/mL 
               
               
                 R-21 
                 721.674 
                 1.4 
                 4 
                 14.6 
                 50 μg/mL 
               
               
                   
               
            
           
         
       
     
     {circle around (2)} 10 μL of the processed sample is taken and uniformly mixed with 2μL of 6×DNA Loading Buffer, it is operated on ice, and a label is made; 
     {circle around (3)} a 8% non-denaturing PAGE gel is taken, a piece of the gel is applied on the samples with different incubation times, 12 μL of the processed samples are all loaded, and a program is set to run the gel at 100V for 40 min; and 
     {circle around (4)} after running the gel, dyeing is performed, it is placed on a horizontal shaker and shaken slowly for 30 min, pictures are taken for imaging. 
     An electrophoresis detection result of the R-15 is shown in  FIG. 32 , an electrophoresis detection result of the R-16 is shown in  FIG. 33 , an electrophoresis detection result of the R-17 is shown in  FIG. 34 , an electrophoresis detection result of the R-18 is shown in  FIG. 35 , an electrophoresis detection result of the R-19 is shown in  FIG. 36 , an electrophoresis detection result of the R-20 is shown in  FIG. 37 , and an electrophoresis detection result of the R-21 is shown in  FIG. 38 . In  FIG. 32 to 38 , lanes from left to right are respectively M: marker, 1: 36 h; 2: 12 h; 3: 1 h; 4: 10 min; 5: 0 min. It may be seen from the results of the serum stability test that: the results of the non-denaturing gel at 10 min, 1 h, 12 h and 36 h show that there is no significant difference between bands of RNA nanoparticle samples at different times, it is indicated that RNA nanoparticles R-15 to R-21 are relatively stable in a 1640 medium of 50% FBS without apparent degradation. 
     Embodiment 12 
     A non-denaturing PAGE method is used to characterize the stability of 7 groups of extension fragment deformation+core short-sequence DNA nanoparticles in serum. 
     Main reagents and instruments are as follows: 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 92 
               
               
                   
                   
               
               
                   
                 Reagent name 
                 Article number 
                 Manufacturer 
               
               
                   
                   
               
             
            
               
                   
                 6xDNA Loading 
                 TSJ010 
                 Qingke Biotechnology 
               
               
                   
                 buffer 
                   
                 Co., Ltd 
               
               
                   
                 20 bp DNA Ladder 
                 3420A 
                 TAKARA 
               
               
                   
                 10000*SolarGelRed 
                 E1020 
                 solarbio 
               
               
                   
                 nucleic acid dye 
                   
                   
               
               
                   
                 8% non-denaturing  
                 / 
                 Self-made 
               
               
                   
                 PAGE gel 
                   
                   
               
               
                   
                 1x TBE Buffer  
                 / 
                 Self-made 
               
               
                   
                 (RNA enzyme-free) 
                   
                   
               
               
                   
                 Serum (FBS) 
                 / 
                 Excel 
               
               
                   
                 RPMI 1640 
                 / 
                 GBICO 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 93 
               
               
                   
               
               
                   
                 Name 
                 Model 
                 Manufacturer 
                   
               
               
                   
               
             
            
               
                   
                 Electrophoresis apparatus 
                 PowerPac Basic 
                 Bio-rad 
                   
               
               
                   
                 Vertical electrophoresis  
                 Mini PROTEAN 
                 Bio-rad 
                   
               
               
                   
                 cell 
                 Tetra Cell 
                   
                   
               
               
                   
                 Discoloration shaker 
                 TS-3D 
                 orbital shaker 
                   
               
               
                   
                 Gel imager 
                 GenoSens1880 
                 Shanghai Qinxiang 
                   
               
               
                   
                   
                   
                 Scientific Instrument  
                   
               
               
                   
                   
                   
                 Co., Ltd. 
               
               
                   
               
            
           
         
       
     
     Steps: 
     {circle around (1)} The DNA nanoparticles are prepared to the concentration in the table below, and then the prepared sample is diluted according to a method in the table below it is diluted by 5 tubes. After being diluted, the sample is placed in a water bath at 37 DEG C. for different times (0, 10 min, 1 h, 12 h, and 36 h); 
     
       
         
           
               
               
               
               
               
               
             
               
                 TABLE 94 
               
               
                   
               
               
                   
                 Sample 
                   
                 50% FBS- 
                   
                 Diluted 
               
               
                 Sample 
                 concentration 
                 Sample 
                 1640/ 
                 1640/ 
                 concentration 
               
               
                 name 
                 (μg/mL) 
                 volume 
                 μL 
                 μL 
                 (μg/mL) 
               
               
                   
               
             
            
               
                 D-8  
                 2890.932 
                 1.4 
                 8 
                 30.6 
                 100 
               
               
                 D-9  
                 2238.682 
                 1.8 
                 8 
                 30.2 
                 100 
               
               
                 D-10 
                 2075.084 
                 1.9 
                 8 
                 30.1 
                 100 
               
               
                 D-11 
                 3117.389 
                 1.3 
                 8 
                 30.7 
                 100 
               
               
                 D-12 
                 2880.939 
                 1.4 
                 8 
                 30.6 
                 100 
               
               
                 D-13 
                 2704.757 
                 1.5 
                 8 
                 30.5 
                 100 
               
               
                 D-14 
                 3216.917 
                 1.2 
                 8 
                 30.8 
                 100 
               
               
                   
               
            
           
         
       
     
     {circle around (2)} 10 μL of the processed sample is taken and uniformly mixed with 2 μL of 6×DNA Loading Buffer, it is operated on ice, and a label is made; 
     {circle around (3)} a 8% non-denaturing PAGE gel is taken, a piece of the gel is applied on the samples with different incubation times, 6 μL of the processed samples are all loaded, and a program is set to run the gel at 100V for 40 min; and 
     {circle around (4)} after running the gel, dyeing is performed, it is placed on a horizontal shaker and shaken slowly for 30 min, pictures are taken for imaging. 
     An electrophoresis detection result of the D-8 is shown in  FIG. 39 , an electrophoresis detection result of the D-9 is shown in  FIG. 40 , an electrophoresis detection result of the D-10 is shown in  FIG. 41 , an electrophoresis detection result of the D-11 is shown in  FIG. 42 , an electrophoresis detection result of the D-12 is shown in  FIG. 43 , an electrophoresis detection result of the D-13 is shown in  FIG. 44 , and an electrophoresis detection result of the D-14 is shown in  FIG. 45 . In  FIG. 39 to 45 , lanes from left to right are respectively M: marker, 1: 36 h; 2: 12 h; 3: 1 h; 4: 10 min; 5: 0 min. It may be seen from the results of the serum stability test that: the results of the non-denaturing gel at 10 min, 1 h, 12 h and 36 h show that there is no significant difference between bands of DNA nanoparticle samples at different times, it is indicated that DNA nanoparticles D-8 to D-14 are relatively stable in a 1640 medium of 50% FBS without apparent degradation. 
     Drug loading test of nucleic acid nanoparticle 
     Embodiment 13 
     Doxorubicin Loading Experiment: 
     According to the chemical loading method (unless otherwise specified, the method is the same as that of Embodiment 5) of Embodiment 5, the RNA nanoparticles formed by the self-assembly of the previous R-15, R-16, R-17, R-18, R-19, R-20 and R-21 in Embodiment 9, and the DNA nanoparticles formed by the self-assembly of the D-8, D-9, D-10, D-11, D-12, D-13 and D-14 in Embodiment 10 are respectively used as doxorubicin loading carriers, doxorubicin loading rates measured are respectively as follows: 
     The doxorubicin loading rate of RNA nanoparticles R-15 is 20.5. 
     The doxorubicin loading rate of RNA nanoparticles R-16 Is 29.4. 
     The doxorubicin loading rate of RNA nanoparticles R-17 is 30.9. 
     The doxorubicin loading rate of RNA nanoparticles R-18 is 34.1. 
     The doxorubicin loading rate of RNA nanoparticles R-19 is 27.1. 
     The doxorubicin loading rate of RNA nanoparticles R-20 is 30.2. 
     The doxorubicin loading rate of RNA nanoparticles R-21 is 20.1. 
     The doxorubicin loading rate of DNA nanoparticles D-8 is 28.0. 
     The doxorubicin loading rate of DNA nanoparticles D-9 is 27.9. 
     The doxorubicin loading rate of DNA nanoparticles D-10 is 18.9. 
     The doxorubicin loading rate of DNA nanoparticles D-11 is 26.8. 
     The doxorubicin loading rate of DNA nanoparticles D-12 is 27.6. 
     The doxorubicin loading rate of DNA nanoparticles D-13 is 31.8. 
     The doxorubicin loading rate of DNA nanoparticles D-14 is 32. 
     Cell binding ability of DNA nanoparticles and carrier drugs detected by Fluorescence Activated Cell Sorter (FACS) Experiment 
     Embodiment 14 
     I. Cell Information 
     HepG2 (from Concord Cell Bank), a medium is DMEM+10% FBS+1% double antibody (gibco, 15140-122), and culture conditions are 37 DEG C., 5% CO 2  and saturated humidity. 
     II. Substances to be Tested 
     Blank carrier: DNA nanoparticle carriers formed by the self-assembly of the previous D-8, D-9, D-10, D-11, D-12, D-13 and B-14 in Embodiment 12. 
     Carrier drug: according to the chemical loading method (unless otherwise specified, the method is the same as that of Embodiment 5) of Embodiment 5, the DNA nanoparticles formed by the self-assembly of the previous D-8, D-9, D-10, D-11, D-12, D-13 and 0-14 in Embodiment 12 are used to load the doxorubicin, they are respectively marked as D-8-doxorubicin, 0-9-doxorubicin, D-10-doxorubicin, 0-11-doxorubicin, D-12-doxorubicin, D-13-doxorubicin and D-14-doxorubicin. 
     III. Main Devices and Consumables 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 95 
               
               
                   
               
               
                   
                   
                 Manufacturer 
                 Model 
                   
               
               
                   
               
             
            
               
                   
                 Biosafety cabinet 
                 Beijing Donglian  
                 BSC-1360  
                   
               
               
                   
                   
                 Haar Instrument 
                 II A2 
                   
               
               
                   
                   
                 Manufacturing Company 
                   
                   
               
               
                   
                 Low-speed centrifuge 
                 Zhongke Zhongjia  
                 SC-3612 
                   
               
               
                   
                   
                 Instrument Co., Ltd. 
                   
                   
               
               
                   
                 CO 2  Incubator 
                 Thermo 
                 3111 
                   
               
               
                   
                 Inverted microscope 
                 UOP 
                 DSZ2000X 
                   
               
               
                   
                 Fluorescence activated 
                 BD 
                 BD 
                   
               
               
                   
                 cell sorter 
                   
                 FACSCalibur ™ 
               
               
                   
               
            
           
         
       
     
     IV. Main Reagent 
     
       
         
           
               
               
               
               
               
               
             
               
                 TABLE 96 
               
               
                   
               
               
                   
                 Reagent 
                   
                 Article 
                   
                   
               
               
                   
                 name 
                 Manufacturer 
                 number 
                 Remark 
               
               
                   
               
             
            
               
                   
                 DMEM 
                 Provided by Baiyao Zhidao 
                 YS3160 
                   
                   
               
               
                   
                 (biotin-free) 
                 Nano-biotechnology Co., Ltd. 
                   
                   
                   
               
               
                   
                 1% BSA-PBS 
                 Self-made 
                 — 
               
               
                   
               
            
           
         
       
     
     V. Experiment Method 
     1. A cell state is adjusted to a logarithmic growth phase, the medium is changed into a medium without biotin and folic acid, and it is incubated overnight in an incubator at 37 DEG C. 
     2. After incubation, cell suspension is trypsinized and collected, and centrifuged at 1000 rpm for 5 min, after the concentration is adjusted, a 2×10 6 -5×10 6  cell/EP tube is taken and washed twice with 1 mL/tube of 1% BSA-PBS, and cells at the bottom of the tube is observed to prevent them from being absorbed. 
     3. A substance to be tested is dissolved, and the substance to be tested is diluted to a use concentration. 
     4. Cell supernatant is absorbed, 100 μL of a corresponding sample is added to each tube in order, light is avoided, and it is incubated at 37 DEG C. for 2 h. 
     5. It is washed twice with 1% BSA-PBS; and centrifuged at 1000 rpm for 5 min. 
     6. Finally, it is precipitated with 300 μL of PBS cell resuspension, and flow Cytometric on-machine detection (the blank carrier used in the present embodiment is labeled by Quasar670, and the doxorubicin in the carrier drug has its own fluorescence, so the detection may be performed through FL4-APC and FL2-PE respectively) is performed. 
     7. Data analysis. 
     VI. Experiment Result 
     1. An experiment result is shown in the table below: 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 97 
               
               
                   
               
               
                   
                   
                 Experiment 
                 Positive 
                   
               
               
                   
                 Detected substance 
                 cell 
                 rate 
               
               
                   
               
             
            
               
                   
                 PBS 
                 HepG2 
                  2.11% 
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 D-8-doxorubicin  
                 1 μM 
                 HepG2 
                 93.1% 
                   
               
               
                   
                 (carrier drug) 
                 2 μM 
                 HepG2 
                 96.3% 
                   
               
               
                   
                 D-8 (blank carrier) 
                 1 μM 
                 HepG2 
                 96.9% 
                   
               
               
                   
                   
                 2 μM 
                 HepG2 
                 98.4% 
                   
               
               
                   
                 D-9-doxorubicin  
                 1 μM 
                 HepG2 
                 88.6% 
                   
               
               
                   
                 (carrier drug) 
                 2 μM 
                 HepG2 
                 95.4% 
                   
               
               
                   
                 D-9 (blank carrier) 
                 1 μM 
                 HepG2 
                 96.7% 
                   
               
               
                   
                   
                 2 μM 
                 HepG2 
                 98.1% 
                   
               
               
                   
                 D-10-doxorubicin 
                 1 μM 
                 HepG2 
                 89.4% 
                   
               
               
                   
                 (carrier drug) 
                 2 μM 
                 HepG2 
                 95.5% 
                   
               
               
                   
                 D-10 (blank carrier) 
                 1 μM 
                 HepG2 
                 97.9% 
                   
               
               
                   
                   
                 2 μM 
                 HepG2 
                 98.3% 
                   
               
               
                   
                 D-11 -doxorubicin  
                 1 μM 
                 HepG2 
                 89.3% 
                   
               
               
                   
                 (carrier drug) 
                 2 μM 
                 HepG2 
                 95.5% 
                   
               
               
                   
                 D-11 (blank carrier) 
                 1 μM 
                 HepG2 
                 97.7% 
                   
               
               
                   
                   
                 2 μM 
                 HepG2 
                 97.8% 
                   
               
               
                   
                 D-12-doxorubicin 
                 1 μM 
                 HepG2 
                 94.6% 
                   
               
               
                   
                 (carrier drug) 
                 2 μM 
                 HepG2 
                 95.9% 
                   
               
               
                   
                 D-12 (blank carrier) 
                 1 μM 
                 HepG2 
                 96.9% 
                   
               
               
                   
                   
                 2 μM 
                 HepG2 
                 97.1% 
                   
               
               
                   
                 D-13-doxorubicin  
                 1 μM 
                 HepG2 
                 89.6% 
                   
               
               
                   
                 (carrier drug) 
                 2 μM 
                 HepG2 
                 94.0% 
                   
               
               
                   
                 D-13 (blank carrier) 
                 1 μM 
                 HepG2 
                 97.6% 
                   
               
               
                   
                   
                 2 μM 
                 HepG2 
                 98.2% 
                   
               
               
                   
                 D-14-doxorubicin 
                 1 μM 
                 HepG2 
                 90.3% 
                   
               
               
                   
                 (carrier drug) 
                 2 μM 
                 HepG2 
                 96.1% 
                   
               
               
                   
                 D-14 (blank carrier) 
                 1 μM 
                 HepG2 
                 97.4% 
                   
               
               
                   
                   
                 2 μM 
                 HepG2 
                 98.3% 
               
               
                   
               
            
           
         
       
     
     2. Conclusion 
     1. After the HepG2 cells are incubated with D-8-doxorubicin (carrier drug) and D-8 (blank carrier), the binding rates are very high (93.1%-98.4%). 
     2. After the HepG2 cells are incubated with D-9-doxorubicin (carrier drug) and D-9 (blank carrier), the binding rates are very high (88.6%-98.1%). 
     3. After the HepG2 cells are incubated with D-10-doxorubicin (carrier drug) and D-10 (blank carrier), the binding rates are very high (89.4%-98.3%). 
     4. After the HepG2 cells are incubated with D-11-doxorubicin (carrier drug) and D-11 (blank carrier), the binding rates are very high (89.3%-97.8%). 
     5. After the HepG2 cells are incubated with D-12-doxorubicin (carrier drug) and D-12 (blank carrier), the binding rates are very high (94.6%-97.1%). 
     6. After the HepG2 cells are incubated with D-13-doxorubicin (carrier drug) and D-13 (blank carrier), the binding rates are very high (89.6%-98.2%). 
     7. After the HepG2 cells are incubated with D-14-doxorubicin (carrier drug) and D-14 (blank carrier), the binding rates are very high (90.3%-98.3%). 
     Cytotoxicity research of DNA nanoparticles and carrier drugs in HepG2 cells 
     Embodiment 16 
     A CCK8 method is used to detect the toxicity of DNA nanoparticles and carrier drugs to HepG2. 
     I. Main Reagent 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 98 
               
               
                   
               
               
                   
                 Reagent name 
                 Manufacturer 
                 Article number 
                   
               
               
                   
               
             
            
               
                   
                 PBS 
                   
                   
                   
               
               
                   
                 DMSO 
                 SIGMA 
                 D2650 
                   
               
               
                   
                 DMEM(biotin- 
                 Provided by Baiyao Zhidao  
                 YS3160 
                   
               
               
                   
                 free) 
                 Nano-biotechnology Co., Ltd. 
                   
                   
               
               
                   
                 FBS 
                 Excell Bio 
                 FSP500 
                   
               
               
                   
                 Double-antibody 
                 gibco 
                 15140-122 
                   
               
               
                   
                 pancreatin 
                 gibco 
                 25200-056 
                   
               
               
                   
                 CCK8 kit 
                 Biyuntian Company 
                 C0038 
               
               
                   
               
            
           
         
       
     
     II. Main Consumables and Instruments 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 99 
               
               
                   
               
               
                   
                 Name 
                 Manufacturer 
                 Model 
                   
               
               
                   
               
             
            
               
                   
                 96-well cell 
                 NEST 
                 701001 
                   
               
               
                   
                 culture plate 
                   
                   
                   
               
               
                   
                 Biosafety cabinet 
                 Beijing Donglian Haer Instrument  
                 BSC-1360  
                   
               
               
                   
                   
                 Manufacturing Company 
                 II A2 
                   
               
               
                   
                 Low-speed  
                 Zhongke Zhongjia Instrument  
                 SC-3612 
                   
               
               
                   
                 centrifuge 
                 Co., Ltd. 
                   
                   
               
               
                   
                 CO 2  Incubator 
                 Thermo 
                 3111 
                   
               
               
                   
                 Inverted microscope 
                 UOP 
                 DSZ2000X 
                   
               
               
                   
                 Microplate reader 
                 Shanghai Ouying Experimental  
                 K3 
                   
               
               
                   
                   
                 Equipment Co., Ltd. 
               
               
                   
               
            
           
         
       
     
     III. Cell Information 
     HepG2 (from Concord Cell Bank), a medium is DMEM+10% FBS+1% double antibody (gibco, 15140-122), and culture conditions are 37 DEG C., 5% CO 2  and saturated humidity. 
     IV. Experiment Material 
     1. Samples to be Tested 
     Blank carrier: DNA nanoparticle carriers formed by the self-assembly of the previous D-8, D-9, D-10, D-11, D-12, D-13 and D-14 In Embodiment 10, respectively marked as: D-8, D-9, D-10, D-11, D-12, D-13 and D-14. 
     Carrier drug: according to the chemical loading method (unless otherwise specified, the method is the same as that of Embodiment 5) of Embodiment 5, the DNA nanoparticles formed by the self-assembly of the previous D-8, D-9, D-10, D-11, D-12. D-13 and D-14 in Embodiment 10 are used to load a doxorubicin, they are respectively marked as D-8-doxorubicin, D-9-doxorubicin, D-10-doxorubicin, D-11-doxorubicin. D-12-doxorubicin, D-13-doxorubicin and D-14-doxorubicin. 
     Original drug doxorubicin. 
     DMSO. 
     V. Experiment Procedure 
     1. HepG2 cells in a logarithmic growth phase are taken, trypan blue is used for staining and the cell viability is counted to be 98.3%, plating is performed with 5000 Cell/well, a volume is 100 μL/well, 8 96-well plates are paved with 57 wells per plate, and it is incubated overnight at 37 DEG C. 
     2. A sample to be tested is diluted according to the following table and added: the original culture medium is removed and 100 μL of a culture medium of the sample to be tested with the different concentration is added, and there are 3 replicate wells in each group. 
     
       
         
           
               
               
               
               
               
               
               
               
               
               
             
               
                 TABLE 100 
               
               
                   
               
               
                 Well number 
                 C9 
                 C8 
                 C7 
                 C6 
                 C5 
                 C4 
                 C3 
                 C2 
                 C1 
               
               
                   
               
             
            
               
                 Final concentration 
                 10 μM 
                 3.16 μM 
                  1 μM 
                  316 nM 
                 100 nM 
                 31.6 nM 
                 10 nM 
                  3.16 nM 
                   1 nM 
               
               
                 of loaded drug 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 Final concentration 
                  1 μM 
                  316 nM 
                 100 nM 
                 31.6 nM 
                  10 nM 
                 3.16 nM 
                  1 nM 
                 0.316 nM 
                 0.1 nM 
               
               
                 of blank carrier 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 Final concentration 
                 10 μM 
                 3.16 μM 
                  1 μM 
                  316 nM 
                 100 nM 
                 31.6 nM 
                 10 nM 
                  3.16 nM 
                   1 nM 
               
               
                 of original drug 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 doxorubicin 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 DMSO (%) 
                 0.1 
                 0.0316 
                 0.01 
                 0.00316 
                 0.001 
                 0.00036 
                 0.0001 
                 0.000036 
                 0.00001 
               
               
                   
               
            
           
         
       
     
     In the present embodiment, the loaded drug and the blank carrier are firstly prepared into 100 μM of stock solution using the PBS, and then diluted with the complete culture medium (biotin-free DMEM). The original drug doxorubicin is firstly prepared into 100 NM of stock solution by using the DMSO, and then diluted with the complete culture medium (biotin-free DMEM). The DMSO is directly diluted with the complete medium (biotin-free DMEM). 
     3. After the sample to be tested is added, the 96-well plate is placed in an incubator under 37 DEG C. and 5% CO 2  and incubated for 72 hours. 
     4. A kit is taken out and melted at a room temperature, 10 μL of CCK-8 solution is added to each wall, or the CCK8 solution is nixed with the culture medium at a ratio of 1:9, and then added to the well in an amount of 100 μL/well. 
     5. It is continuously incubated in the cell incubator for 4 hours, and a length of time depends on experimental conditions such as a cell type and a el density. 
     6. A microplate reader is used to measure an absorbance at 450 nm. 
     7. Calculation: cell viability (%)=(OD experimental group−OD blank group)×1000%/(OD control group−OD blank group), IC 50  is calculated by GraphPad Prism 5.0. 
     VI. Experiment Result 
     
       
         
           
               
               
               
             
               
                 TABLE 101 
               
               
                   
               
               
                 Cell line 
                 Detected sample 
                 IC 50  (μM) 
               
               
                   
               
             
            
               
                 HepG2 
                 Original drug doxorubicin 
                 0.2725 
               
               
                   
                 D-8-doxorubicin (loaded drug) 
                 0.05087 
               
               
                   
                 D-8 (blank carrier) 
                 &gt;1 
               
               
                   
                 D-9-doxorubicin (loaded drug) 
                 0.0386 
               
               
                 HepG2 
                 D-9 (blanking carrier) 
                 &gt;1 
               
               
                   
                 D-10-doxorubicin (loaded drug) 
                 0.03955 
               
               
                   
                 D-10 (blank carrier) 
                 &gt;1 
               
               
                   
                 D-11-doxorubicin (loaded drug) 
                 0.04271 
               
               
                 HepG2 
                 D-11 (blank carrier) 
                 &gt;1 
               
               
                   
                 D-12-doxorubicin (loaded drug) 
                 0.02294 
               
               
                   
                 D-12 (blank carrier) 
                 &gt;1 
               
               
                   
                 D-13-doxoru biciri (loaded drug) 
                 0.03017 
               
               
                 HepG2 
                 D-13 (blank carrier) 
                 &gt;1 
               
               
                   
                 D-14-doxorubicin (loaded drug) 
                 0.03458 
               
               
                   
                 D-14 (blank carrier) 
                 &gt;1 
               
               
                   
                 DMSO 
                 &gt;0.1% 
               
               
                   
               
            
           
         
       
     
     Conclusion: 
     It may be seen from the above table and  FIG. 46 a   ,  FIG. 46 b   ,  FIG. 48 c   ,  FIG. 46 d   ,  FIG. 46 e   ,  FIG. 49 .  FIG. 46 g    and  FIG. 46 h    that the IC 50  of the original drug doxorubicin and the loaded drugs D-8-doxorubicin, D-9-doxorubicin, D-10-doxorubicin, D-11-doxorubicin, D-12-doxorubicin, D-13-doxorubicin and D-14-doxorubicin acting on the HepG2 cells is 0.2725 μM, 0.05087 μM, 0.0386, 0.03955, 0.04271, 0.02294, 0.03017 and 0.03458 respectively; the IC 5  of the DMSO acting on the HepG2 cells is &gt;0.1%; the IC N  of the D-8 (blank carrier), D-9 (blank carrier), D-10 (blank carrier), D-11 (blank carrier), D-12 (blank carrier), D-13 (blank carrier) and D-14 (blank carrier) acting on the HepG2 cells is &gt;1 μM. It is indicated that for the HepG2 cell line, compared with the simple blank carriers D-8, D-9, D-10, D-11, D-12, D-13 and D-14, the original drug doxorubicin of small molecular drug and the loaded drugs D-8-doxorubicin, D-9-doxorubicin, D-10-doxorubicin, D-11-doxorubicin, D-12-doxorubicin, D-13-doxorubicin Both 0-14-doxorubicin and D-14-doxorubicin are toxic to the HepG2 cells, and compared with the original drug doxorubicin, the loaded drugs D-8-doxorubicin, D-9-doxorubicin, D-10-doxorubicin, and D-11-doxorubicin, D-12-doxorubicin, D-13-doxorubicin and D-14-doxorubicin have an apparent synergistic effect. 
     Embodiment 16 
     According to the chemical loading method (unless otherwise specified, the method is the same as that of Embodiment 5) of Embodiment 5, the DNA nanoparticles formed by the self-assembly of the previous D-10 and D-14 in Embodiment 10 are used as a daunorubicin loading carrier. A microplate reader is used to measure an absorbance of the daunorubicin at 492 nm, and a standard curve (as shown in  FIG. 47 ) is drawn. 
     Measured loading rates of the daunorubicin are respectively as follows: 
     The daunorubicin loading rate of the DNA nanoparticles D-10 is 24.0. 
     The daunorubicin loading rate of the DNA nanoparticles D-14 is 25.1. 
     Embodiment 17 
     Epirubicin Loading Experiment 
     (I) Loading of RNA Nanoparticles 
     According to a chemical loading method of Embodiment 5 (unless otherwise specified, the method is the same as that of Embodiment 5, the same molar number of the epirubicin is used as a loading amount), nucleic acid nanoparticles (a molecular weight is 29550, it is similar to the RNA nanoparticles in Embodiment 1, and a difference is that a fluorescent label on a c-strand is Cy5) are used as a carrier, and respectively loaded with: epirubicin, methotrexate, pirarubicin, daunorubicin, pentafluorouracil, 10-hydroxycamptothecin, aspirin and gemcitabine. 
     Herein, when a standard curve is drawn, the absorbance of the above drugs on the microplate reader is measured respectively. The absorbance of the epirubicin, methotrexate, pirarubicin, daunorubicin, pentafluorouracil, 10-hydroxycamptothecin, aspirin and gemcitabine is respectively detected at the following wavelengths: 492 nm, 303 nm, 492 nm, 492 nm, 265 nm, 384 nm, 225 nm and 268 nm. The standard curves obtained correspondingly are respectively shown in  FIG. 48 a   ,  FIG. 49 ,  FIG. 50   sa ,  FIG. 51 ,  FIG. 52 ,  FIG. 53 ,  FIG. 54 a    and  FIG. 55 . 
     Each loading rate measured is respectively as follows: 
     
       
         
           
             Epirubicin 
             ⁢ 
             
               : 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   1 
                 
               
               = 
               
                 21.0 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   μ 
                 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   epirubicin 
                   - 
                   1 
                 
               
               = 
               
                 7.158 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   2 
                 
               
               = 
               
                 33.5 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   μ 
                 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   epirubicin 
                   - 
                   2 
                 
               
               = 
               
                 9.263 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 1 
               
               = 
               
                 
                   
                     7.158 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0210 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 10.2 
               
             
             ; 
           
         
       
       
         
           
             
               N 
               ⁢ 
               
                 - 
               
               ⁢ 
               2 
             
             = 
             
               
                 
                   9.263 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                 
                 
                   0.0335 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   
                     6 
                     / 
                     30000 
                   
                 
               
               = 
               
                 8.3 
                 . 
               
             
           
         
       
     
     An average value thereof is taken so that the loading rate of epirubicin-RNAh nucleic acid nanoparticles is about 9.3, and it means that about 9.3 epirubicin molecules may be loaded on each nucleic acid nanoparticle carrier. 
     
       
         
           
             
                 
             
             ⁢ 
             
               Methotrexate 
               ⁢ 
               
                 : 
               
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   1 
                 
               
               = 
               
                 45.0 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   μ 
                 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   methotrexate 
                   - 
                   1 
                 
               
               = 
               
                 16.9 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   2 
                 
               
               = 
               
                 36.0 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   μ 
                 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   methotrexate 
                   - 
                   2 
                 
               
               = 
               
                 10.85 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 2 
               
               = 
               
                 
                   
                     10.85 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0360 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 9.04 
               
             
             ; 
           
         
       
       
         
           
             
               N 
               ⁢ 
               
                 - 
               
               ⁢ 
               1 
             
             = 
             
               
                 
                   16.9 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                 
                 
                   0.0450 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   
                     6 
                     / 
                     30000 
                   
                 
               
               = 
               
                 11.3 
                 . 
               
             
           
         
       
     
     An average value thereof is taken so that the loading rate of methotrexate-RNAh nucleic acid nanoparticles is about 10, and it means that about 10 methotrexate molecules may be loaded on each nucleic acid nanoparticle carrier. 
     
       
         
           
             Pirarubicin 
             ⁢ 
             
               : 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   1 
                 
               
               = 
               
                 23.2 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   pirarubicin 
                   - 
                   1 
                 
               
               = 
               
                 8.500 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   2 
                 
               
               = 
               
                 48.1 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   pirarubicin 
                   - 
                   2 
                 
               
               = 
               
                 19.24 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 1 
               
               = 
               
                 
                   
                     8.500 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0232 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 11 
               
             
             , 
             
               
 
             
             ⁢ 
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 2 
               
               = 
               
                 
                   
                     19.24 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0481 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 12. 
               
             
           
         
       
     
     An average value thereof is taken so that the loading rate of pirarubicin-RNAh nucleic acid nanoparticles is about 11.5, and it means that about 11.5 pirarubicin molecules may be loaded on each nucleic acid nanoparticle carrier. 
     
       
         
           
             Daunorubicin 
             ⁢ 
             
               : 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   1 
                 
               
               = 
               
                 58.8 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   daunorubicin 
                   - 
                   1 
                 
               
               = 
               
                 11.76 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   2 
                 
               
               = 
               
                 39.8 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   daunorubicin 
                   - 
                   2 
                 
               
               = 
               
                 7.506 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 1 
               
               = 
               
                 
                   
                     11.76 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0588 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 6 
               
             
             , 
             
               
 
             
             ⁢ 
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 2 
               
               = 
               
                 
                   
                     7.506 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0398 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 
                   5.7 
                   . 
                 
               
             
           
         
       
     
     An average value thereof is taken so that the loading rate of daunorubicin-RNAh nucleic acid nanoparticles is about 6, and it means that about 6 daunorubicin molecules may be loaded on each nucleic acid nanoparticle carrier. 
     Pentafluorouracil: 
     The loading rate of the RNAh-pentafluorouracil obtained by calculating is about 0.31, and it is represented that about 0.31 pentafluorouracil molecules may be loaded on each nucleic acid nanoparticle carrier. 
     Through changing a relative dosage of the pentafluorouracil and the RNA nanoparticles, the RNAh-pentafluorouracil particles of which the loading rates is 10, 20, 28, and 50 and the like may also be acquired, it is not repeatedly described here. 
     
       
         
           
             10 
             ⁢ 
             
               - 
             
             ⁢ 
             hydroxycamptothecin 
             ⁢ 
             
               : 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   1 
                 
               
               = 
               
                 73.3 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   
                     
                       1 
                       ⁢ 
                       0 
                     
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     hydroxycamptothecin 
                   
                   ⁢ 
                   
                       
                   
                   - 
                   1 
                 
               
               = 
               
                 28.88 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   2 
                 
               
               = 
               
                 65.8 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     hydroxycamptothecin 
                   
                   ⁢ 
                   
                       
                   
                   - 
                   2 
                 
               
               = 
               
                 15.2 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 2 
               
               = 
               
                 
                   
                     15.2 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0658 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 6.9 
               
             
             , 
             
               
 
             
             ⁢ 
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 1 
               
               = 
               
                 
                   
                     28.88 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0733 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 
                   11.8 
                   . 
                 
               
             
           
         
       
     
     An average value thereof is taken so that the loading rate of 10-hydroxycamptothecin-RNAh is about 9, and it means that about 16 10-hydroxycamptothecin molecules may be loaded on each nucleic acid nanoparticle carrier. 
     Through changing a relative dosage of the 10-hydroxycamptothecin and the RNA nanoparticles, the RNAh-10-hydroxycamptothecin particles of which the loading rates is 10, 20, 28, 50, 70, 80, 100, and 200 and the like may also be acquired, it is not repeatedly described here. 
     
       
         
           
             Aspirin 
             ⁢ 
             
               : 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   1 
                 
               
               = 
               
                 68.4 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   
                     a 
                     ⁢ 
                     spirin 
                   
                   - 
                   1 
                 
               
               = 
               
                 52.5 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   2 
                 
               
               = 
               
                 26.8 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   
                     a 
                     ⁢ 
                     spirin 
                   
                   - 
                   2 
                 
               
               = 
               
                 18.4 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 2 
               
               = 
               
                 
                   
                     18.4 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0268 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 20.59 
               
             
             , 
             
               
 
             
             ⁢ 
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 1 
               
               = 
               
                 
                   
                     52.5 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0684 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 
                   23.02 
                   . 
                 
               
             
           
         
       
     
     An average value of the N-1 and N-2 is taken so that the loading rate of aspirin-RNAh is about 22, and it means that about 22 aspirin molecules may be loaded on each nucleic acid nanoparticle carrier. 
     
       
         
           
             Gemcitabine 
             ⁢ 
             
               : 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   1 
                 
               
               = 
               
                 26.9 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   gemcitabine 
                   - 
                   1 
                 
               
               = 
               
                 18.23 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   RNAh 
                   - 
                   2 
                 
               
               = 
               
                 29.8 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 RNAh 
               
               ≈ 
               30000 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   gemcitabine 
                   - 
                   2 
                 
               
               = 
               
                 21.65 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 2 
               
               = 
               
                 
                   
                     21.65 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0298 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 21.8 
               
             
             , 
             
               
 
             
             ⁢ 
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 1 
               
               = 
               
                 
                   
                     18.23 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.0269 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       30000 
                     
                   
                 
                 = 
                 
                   20.4 
                   . 
                 
               
             
           
         
       
     
     An average value of the N-1 and N-2 is taken so that the loading rate of gemcitabine-RNAh is about 21, and it means that about 21 gemcitabine molecules may be loaded on each nucleic acid nanoparticle carrier. 
     (II) Loading Experiment of DNA Nucleic Acid Nanoparticles 
     The loading method and the calculation method of the loading rate are the same as the above RNA nucleic acid nanoparticles. The specific nucleic acid nanoparticles used are: DNAh-Bio-EGFRapt-Cy5, herein the three strands of DNAh are respectively as follows: 
     a-strand: (SEQ ID NO:172:) 5′-CGCGCGCCCACGAGCGTTCCGGGCGCGCCTTAGTAACGTGCTTTGATGTCGATTCGACAG GAGGC-3′; the first three bases at the 5′-end and the last three bases at the 3′-end are thio-modified, and the 5′-end is linked with Biotin, a bolded part is the EGFRapt sequence; 
     b-strand (SEQ ID NO: 173:): 5′-GCGCCCGGTTCGCCGCCAGCCGCCGC-3′, the first three bases at the 5′-end and the last three bases at the 3′-end are thio-modified; and 
     c-strand (SEQ ID NO: 174:): 5′-GCGGCGGCAGGCGGCCATAGCCGTGGGCGCGCG-3′; the first three bases at the 5-end and the last three bases at the 3-end are respectively thio-modified, and the 3′-end is linked with a Cy5 fluorescent label. 
     The standard curve of the above epirubicin-loaded DNA nucleic acid nanoparticles is shown in  FIG. 48 b   , and a specific calculation process is as follows: 
     
       
         
           
             
               
                 C 
                 
                   DNAh 
                   - 
                   1 
                 
               
               = 
               
                 22.19 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 DNAh 
               
               ≈ 
               39500 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   epirubicin 
                   - 
                   1 
                 
               
               = 
               
                 17.06 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   DNAh 
                   - 
                   2 
                 
               
               = 
               
                 32.57 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 DNAh 
               
               ≈ 
               39500 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   epirubicin 
                   - 
                   2 
                 
               
               = 
               
                 20.40 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               N 
               ⁢ 
               
                 - 
               
               ⁢ 
               1 
             
             = 
             
               
                 
                   17.06 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                 
                 
                   0.2219 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   
                     6 
                     / 
                     39500 
                   
                 
               
               ≈ 
               30.4 
             
           
         
       
       
         
           
             
               
                 N 
                 ⁢ 
                 
                   - 
                 
                 ⁢ 
                 2 
               
               = 
               
                 
                   
                     20.40 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     6 
                   
                   
                     0.3257 
                     × 
                     100 
                     × 
                     10 
                     ⁢ 
                     
                       - 
                     
                     ⁢ 
                     
                       6 
                       / 
                       39500 
                     
                   
                 
                 ≈ 
                 24.7 
               
             
             ; 
           
         
       
     
     An average value thereof is taken so that the loading rate of epirubicin-DNAh is about 27.6, and it means that about 27.8 epirubicin molecules may be loaded on each DNA nanoparticle carrier. 
     The standard curve of the above pirarubicin-loaded DNA nucleic acid nanoparticles is shown in  FIG. 50 b   , and a specific calculation process is as follows: 
     
       
         
           
             
               
                 C 
                 
                   DNAh 
                   - 
                   1 
                 
               
               = 
               
                 18.64 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 DNAh 
               
               ≈ 
               39500 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   pirarubicin 
                   - 
                   1 
                 
               
               = 
               
                 11.7 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   DNAh 
                   - 
                   2 
                 
               
               = 
               
                 41.23 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 DNAh 
               
               ≈ 
               39500 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   pirarubicin 
                   - 
                   2 
                 
               
               = 
               
                 19.73 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               N 
               ⁢ 
               
                 - 
               
               ⁢ 
               1 
             
             = 
             
               
                 
                   11.7 
                   ⁢ 
                   
                       
                   
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                 
                 
                   0.1864 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   
                     6 
                     / 
                     39500 
                   
                 
               
               ≈ 
               24.9 
             
           
         
       
       
         
           
             
               N 
               ⁢ 
               
                 - 
               
               ⁢ 
               2 
             
             = 
             
               
                 
                   19.73 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                 
                 
                   0.4123 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   
                     6 
                     / 
                     39500 
                   
                 
               
               ≈ 
               
                 18.9 
                 . 
               
             
           
         
       
     
     An average value thereof is taken so that the loading rate of pirarubicin-DNAh is about 21.9, and it means that about 21.9 pirarubicin molecules may be loaded on each DNA nanoparticle carrier. 
     The standard curve of the above aspirin-loaded DNA nucleic acid nanoparticles is shown in  FIG. 54 b   , and a specific calculation process is as follows: 
     
       
         
           
             
               
                 C 
                 
                   DNAh 
                   - 
                   1 
                 
               
               = 
               
                 10.97 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 DNAh 
               
               ≈ 
               39500 
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   aspirin 
                   - 
                   1 
                 
               
               = 
               
                 3.7 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 
                   100 
                   
                       
                   
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   DNAh 
                   - 
                   2 
                 
               
               = 
               
                 21.56 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 
                   g 
                   / 
                   ml 
                 
               
             
             , 
             
               
                 M 
                 DNAh 
               
               ≈ 
               39500 
             
             , 
             
               
                 100 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               
                 C 
                 
                   aspirin 
                   - 
                   2 
                 
               
               = 
               
                 7.67 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 M 
               
             
             , 
             
               
                 100 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 μ 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 L 
               
               ; 
             
           
         
       
       
         
           
             
               N 
               ⁢ 
               
                 - 
               
               ⁢ 
               1 
             
             = 
             
               
                 
                   3.7 
                   ⁢ 
                   
                       
                   
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                 
                 
                   0.1097 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   
                     6 
                     / 
                     39500 
                   
                 
               
               ≈ 
               13.4 
             
           
         
       
       
         
           
             
               N 
               ⁢ 
               
                 - 
               
               ⁢ 
               2 
             
             = 
             
               
                 
                   7.67 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   6 
                 
                 
                   0.2156 
                   × 
                   100 
                   × 
                   10 
                   ⁢ 
                   
                     - 
                   
                   ⁢ 
                   
                     6 
                     / 
                     39500 
                   
                 
               
               ≈ 
               14.05 
             
           
         
       
     
     An average value thereof is taken so that the loading rate of asprin-DNAh is about 14, and it means that about 14 aspirin molecules may be loaded on each DNA nanoparticle carrier. 
     In addition, on the basis of the above RNA nanoparticles and DNA nanoparticles loaded with each of the above drugs such as the epirubicin, other small molecular drugs may be further loaded for the second time in the same way as the epirubicin loading. For example, the present application is further loaded with a folic acid to obtain the RNA nanoparticles and DNA nanoparticles co-loaded with two small molecular drugs of the epirubicin and the folic acid, and the loading rates of the two drugs may be detected by referring to the above method (values are not shown). 
     It is indicated from the present embodiment that the RNA nanoparticles (in Embodiment 1) and DNA nanoparticles with the extension fragment, the target head and the fluorescein have a function of loaded drugs, may achieve the loading with the small molecular drug epirubicin in a mode of covalent linkage (paraformaldehyde-solvent covalence), and may also achieve the co-loading with other small molecular drugs. 
     Embodiment 18 
     Cell Binding Ability of Drug-Loaded RNA Nanoparticles Detected by Confocal Microscopy Experiment 
     I. Experiment Material and Experiment Method: 
     1. Samples to be tested as shown in Table 102: 
     
       
         
           
               
               
               
               
               
             
               
                   
               
               
                   
                   
                   
                 Dissolution 
                   
               
               
                   
                 Nanoparticles 
                 MW 
                 reagent 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 RNAh-Biotin-quasar670, RNAh-Bio-670  
                 29552.6 
                 PBS 
                   
               
               
                   
                 for short 
                   
                   
                   
               
               
                   
                 RNAh-Biotin-quasar670-EPB, RNAh- 
                 35352.4 
                 PBS 
                   
               
               
                   
                 Bio-670-EPB for short 
                   
                   
                   
               
               
                   
                 RNAh-Biotin-quasar670-MTX, RNAh- 
                 35352.4 
                 PBS 
                   
               
               
                   
                 Bio-670-MTX for short 
                   
                   
                   
               
               
                   
                 RNAh-Biotin-quasar670-THP RNAh- 
                 35352.4 
                 PBS 
                   
               
               
                   
                 Bio-670-THP for short 
                   
                   
                   
               
               
                   
                 RNAh- Biotin-quasar670- DNR, RNAh- 
                 35352.4 
                 PBS 
                   
               
               
                   
                 Bio-670-DNR for short 
                   
                   
                   
               
               
                   
                 RNAh-Biotin-quasar670-flu 
                 32934.68 
                 PBS 
                   
               
               
                   
                 RNAh-Biotin-quasar670-hdcp 
                 33196.1 
                 PBS 
                   
               
               
                   
                 RNAh-Biotin-quasar670-aspirin, RNAh- 
                 34056.35 
                 PBS 
                   
               
               
                   
                 Bio-670-aspirin for short, also called  
                   
                   
                   
               
               
                   
                 RNAh-Bio-aspirin 
                   
                   
                   
               
               
                   
                 RNAh- Biotin-quasar670-gemcitabine, 
                 35079.8 
                 PBS 
                   
               
               
                   
                 RNAh-Bio-670-gemcitabine for short,  
                   
                   
                   
               
               
                   
                 also called RNAh- Bio-gemcitabine 
               
               
                   
               
               
                 Note: 
               
               
                 the RNAh-Bio-670 in the table is served as a control, and refers to the nanoparticles formed by performing the Biotin modification at the 5’-end of the a-strand and b-strand prepared according to the self-assembly method in Embodiment 1, and performing the quasar670 fluorescein modification at the 3’-end of the c-strand, and the RNAh- Bio-670- EPB and the like refer to the nano particles formed after further loading the epirubicin (loaded according to the chemical method in Embodiment 5). 
               
            
           
         
       
     
     2. Experiment reagents used and sources thereof are as follows: 
     RPMI-1640 medium (Gibco, C118755001BT-500 mL); Fetal bovine serum (FBS) (ExCell Blo, FNA500-500 mL); Penicillin/Streptomycin (PS) (Gibco, 15140-122-100 mL); PBS buffer solution (Gibco, C20012500ST-500 mL); Trypsin-EDTA (Stemcell, 07901-500 mL); DMSO (Sigma. D5879-1L); Prolong Gold Antifade Mountant (Thermo, P36941-2 mL); and DAPI (Yeasen, 36308ES11-4 mL). 
     3. Experiment Instruments used are as follows: 
     Inverted Microscope (Olympus BX53, U-RFL-T); BD Falcon (Corning, 354118); and Cytospin (TXD3). 
     4. Experiment method: 
     (1) Respectively placing cells used by each drug in a RPMI1640+10% FBS+1% PS medium, and culturing under a condition of 37 DEG C. and 5% CO 2 ; 
     Epirubicin: HL60 cells, acute leukemia cell line; 
     Methotrexate: MOLT4 cells (human acute lymphoblastic leukemia line) 
     Pirubicin: MCF-7 cells (human breast cancer cell line) 
     Daunorubicin: MCF-7 cells 
     Pentafluorouracil: HepG2 cells; 
     10-hydroxycamptothecin: SGC7901; 
     Aspirin: SH-SY5Y cells; 
     Gemcitabine: BxPC-3 cells, 
     (2) Collecting the cells, washing with the PBS, and adding to the 48-wall plate in 1×10 5  cells per well. 
     (3) Incubating the cells with 200 nM and 400 nM of the RNAh-Bio-670 and RNAh-Bio-670-EPB nanoparticles at 37 DEG C. and 5% CO 2  for 1-2 h and 4 h. 
     (4) After the cells are washed with the PBS, adding the cells to a glass side by a centrifugal picture method, treating with the Prolong Gold Antifade Mountant, and keeping overnight at a room temperature. 
     (5) Staining with DAPI for 5 min at the room temperature, and then sealing the glass slide. 
     (6) Using DAPI and FAM channels of the laser scanning confocal microscope to evaluate the cell binding and internalization, taking pictures under the microscope and saving. 
     II. Experiment Result 
     Experiment results are shown in  FIG. 56  to  FIG. 63 . It may be seen from  FIG. 56  that the RNAh-Bio-670 and RNAh-Bio-670-EPB nanoparticles may be both bound and internalized with the cells because they both carry the target head—Biotin. It may be seen that the drug RNAh-Bio-670-EPB nanoparticles containing the epirubicin have a relatively strong binding and internalization ability to the HL60 cells. 
     It may be seen from  FIG. 57  that the RNAh-Bio-670 and RNAh-Bio-670-MTX nanoparticles may be both bound and internalized with the cells because they both carry the target head-Biotin. This result shows that the drug RNAh-Bio-670-MTX nanoparticles containing the methotrexate have a relatively strong binding and internalization ability to the MOLT4 cells. 
     It may be seen from  FIG. 58  that the cell binding and internalization experiment results show that the RNAh-Bio-670 and RNAh-Bio-670-THP nanoparticles may be both bound and internalized with the cells because they both carry the target head—Biotin. This result shows that the drug RNAh-Bio-670-THP nanoparticles containing the pirarubicin have a relatively strong binding and internalization ability to the MCF-7 cells. 
     It may be seen from  FIG. 59  that the cell binding and internalization experiment results show that the RNAh-Bio-670 and RNAh-Bio-670-DNR nanoparticles may be both bound and internalized with the cogs because they both carry the target head—Biotin. This result shows that the drug RNAh-Bio-670-DNR nanoparticles containing the daunorubicin have a relatively strong binding and internalization ability to the MCF-7 cells. 
     It may be seen from  FIG. 60  that the cell binding and internalization experiment results show that the RNAh-Biotin-quasar670 and RNAh-Biotin-quasar670-flu nanoparticles may be both bound and internalized with the cells because they both carry the target head—Biotin. 
     It may be seen from  FIG. 61  that the cell binding and internalization experiment results show that the RNAh-Biotin-quasar670 and RNAh-Biotin-quasar670-hdcp nanoparticles may be both bound and internalized with the cells because they both carry the target head—Biotin. 
     It may be seen from the cell binding and internalization experiment results in  FIG. 62  and  FIG. 63  that the aspirin-RNA nanoparticles have a relatively strong binding and internalization ability to the SH-SY5Y cells, and the gemcitabine-RNA nanoparticles have a relatively strong binding and internalization ability to the BxPC-3 cells. 
     Embodiment 19 
     (I) Binding Ability of DNAh-Bio-EGFRapt-Cy5-EPB Nanoparticles and Cells Detected by Flow Cytometry Experiment 
     I. Cell Information 
     MCF-7 (source: ATCC, and article number: HTB-22), NCI-H1975 (source: ATCC, and article number: CRL-5008); a culture medium is MEM+10% FBS, and culture conditions are 37 DEG C., 5% CO 2 , and saturated humidity. 
     II. Substances to be Tested 
     Targeted drug: DNAh-Bio-EGFRapt-Cy5-EPB (loaded according to the DNA nanoparticle loading method in Embodiment 5). 
     Targeted fluorescent carrier: DNAh-Bio-EGFRapt-Cy5. 
     III. Devices and Consumables 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 103 
               
               
                   
                   
               
               
                   
                 Name 
                 Brand 
                 Article number/Model 
               
               
                   
                   
               
             
            
               
                   
                 24-well plate 
                 Corning 
                 3526 
               
               
                   
                 Centrifuge 
                 Gineek 
                 LD5-2B 
               
               
                   
                 CO 2  incubator 
                 Thermo 
                 3111 
               
               
                   
                 Microwell plate shaker 
                 QILINBEIER 
                 QB-9001 
               
               
                   
                 Microscope 
                 Olympus 
                 IX53 
               
               
                   
                 Multifunctional micro 
                 Bio Tek 
                 Synergy H1 
               
               
                   
                 plate reader 
                   
                   
               
               
                   
                 Flow cytometer 
                 ACEA 
                 Novo Cyte 
               
               
                   
                   
               
            
           
         
       
     
     IV. Reagent 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 104 
               
               
                   
               
               
                   
                 Name 
                 Brand 
                 Article number 
                   
               
               
                   
               
             
            
               
                   
                 RPMI 1640 medium 
                 Provided by Baiyao 
                 YS3160-500 
                   
               
               
                   
                 (without folic 
                 Zhidao Nano- 
                   
                   
               
               
                   
                 acid and biotin) 
                 biotechnology 
                   
                   
               
               
                   
                   
                 Co., Ltd 
                   
                   
               
               
                   
                 MEM medium 
                 Provided by Baiyao 
                 YS4150-500 
                   
               
               
                   
                 (without folic 
                 Zhidao Nano- 
                   
                   
               
               
                   
                 acid and biotin) 
                 biotechnology 
                   
                   
               
               
                   
                   
                 Co., Ltd 
                   
                   
               
               
                   
                 DMEM medium (without 
                 Provided by Baiyao 
                 YS1200-500 
                   
               
               
                   
                 folic acid and biotin) 
                 Zhidao Nano- 
                   
                   
               
               
                   
                   
                 biotechnology 
                   
                   
               
               
                   
                   
                 Co., Ltd 
                   
                   
               
               
                   
                 FBS 
                 Cegrogen 
                 A0500-3018 
                   
               
               
                   
                 GlutaMax 
                 Gibco 
                 35050-061 
                   
               
               
                   
                 trypsin-EDTA digestive  
                 Solarbib 
                 T1320-100ml 
                   
               
               
                   
                 juice (0.25%) 
                   
                   
                   
               
               
                   
                 Penicillin-streptomycin (100X) 
                 Solarbio 
                 P1400 
                   
               
               
                   
                 CellTiter-Glo ® 2.0 
                 Promega 
                 G9243 
               
               
                   
               
            
           
         
       
     
     V. Experiment Method: 
     1. Adjusting a cell state to a logarithmic growth phase, changing the medium to a medium without biotin and folic acid, and incubating overnight in the incubator at 37 DEG C; 
     2. dissolving the substances to be tested and preparing the to-be-tested substance stock solution; 
     3. digesting and collecting the single-cell suspension and counting, adjusting the cell density to 2×10 5 /mL, planting 1 mL/well into the 24-well plate; 
     4. respectively adding the substances to be tested to the corresponding cell wells, herein the final concentration is 2 μM, shaking and mixing uniformly; 
     5. placing the cell plate in the incubator at 37 DEG C. and incubating for 2 hours; 
     6. after the incubation, pancreatin-digesting and collecting the cell suspension; 
     7. centrifugally collecting a cell precipitation, and washing twice with the PBS; 
     8. finally, resuspending the cell precipitation with 300 μL of the PBS, and performing a flow cytometric on-machine detection; 
     9. fluorescence carrier or epirubicin detection channels: excitation light wavelength: 488 nm, and emission light channel: 560 nm; and 
     10. data analysis. 
     VI. Experiment Result 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 105 
               
               
                   
               
               
                   
                 Cell line 
                 Test sample 
                 Binding rate (%) 
                   
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 MCF-7 
                 DNAh-Bio-EGFRapt-Cy5-EPB 
                 99.89 
                   
               
               
                   
                   
                 DNAh-Bio-EGFRapt-Cy5 
                 99.97 
                   
               
               
                   
                   
                 Blank control (medium only) 
                 0.16 
                   
               
               
                   
                 NCI-H1975 
                 DNAh-Bio-EGFRapt-Cy5-EPB 
                 100 
                   
               
               
                   
                   
                 DNAh-Bio-EGFRapt-Cy5 
                 100 
                   
               
               
                   
                   
                 Blank control (medium only) 
                 0.34 
               
               
                   
               
            
           
         
       
     
     It may be seen from the above table that the epirubicin targeted drug DNAh-Bio-EGFRapt-Cy5-EPB may be bound with the MCF-7 cells and NCI-H1975 cells, and the binding rates are both close to 100%; and the targeted fluorescent carrier DNAh-Bio-Cy5 may also be bound with the MCF-7 cells and NCI-H1975 cells, and the binding rates are also 100%. 
     (II) Cell Binding Ability of RNAh-Biotin-Cy5-DNR Nanoparticles Detected by Flow Cytometer 
     I. Samples to be tested 
     Targeted drug: RNAh-Biotin-Cy5-DNR, herein a preparation method of the RNAh-Biotin-Cy5 is the same as that of the RNAh-Biotin-quasar670, and a difference is that the fluorescent substance is replaced by the Cy5 from the quasar670. The RNAh-Biotin-Cy5-DNR is the nanoparticles formed by further loading the DNR on the RNAh-Biotin-Cy5 (loaded according to the method in Embodiment 5). 
     II. Experiment cells and culture conditions (MCF-7 cells, the details are the same as the above, and it is not repeatedly described here) 
     III. Fluorescence detection 
     Conditions of a fluorescence detection are as follows: 
     Excitation light is 640 nm, emission light is 675 nm, a detection height is 7 mm, measured value/data point-10, detection speed: normal, and extension: 100 ms. 
     IV. Detection result 
     
       
         
           
               
               
               
               
             
               
                 TABLE 106 
               
             
            
               
                   
               
               
                   
                   
                 Binding rate (%) 
                 Binding rate (%) 
               
               
                   
                   
                 Treatment time 1 h 
                 Treatment time 2 h 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Test sample 
                 0.2 μM 
                 0.4 μM 
                 0.2 μM 
                 0.4 μM 
               
               
                   
               
               
                 MCF-7 
                 RNAh-Biotin- 
                 39.41% 
                 84.22% 
                 87.14% 
                 94.53% 
               
               
                   
                 Cy5-DNR 
                   
                   
                   
                   
               
               
                   
                 Blank control  
                  0.45% 
                  0.45% 
                  0.24% 
                  0.24% 
               
               
                   
                 (medium only) 
               
               
                   
               
            
           
         
       
     
     It may be seen from the above table that the binding rate of RNAh-Biotin-Cy5-DNR nanoparticles and MCF-7 cells nay be as high as 84% or more in the case tat the treatment time and concentration are appropriate. Compared with the blank control containing the medium only, the RNA drug-loaded particles have a strong ability to bind and internalize with the MCF-7 cells. 
     (III) Cell Binding Ability of Pirarubicin-Loaded DNA Nanoparticles Detected by Flow Cytometer 
     I. Cell Information 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 107 
               
               
                   
               
               
                   
                   
                 Product 
                   
                   
               
               
                   
                   
                 model or 
                   
                   
               
               
                   
                   
                 article  
                   
                 Culture 
               
               
                 Cell line 
                 Source 
                 number 
                 Medium 
                 condition 
               
               
                   
               
             
            
               
                 SKOV3 
                 ATCC 
                 HTB-77 
                 MEM + 10% FBS 
                 37 DEG C., 
               
               
                   
                   
                   
                   
                 5% CO 2 , 
               
               
                 SGC-7901 
                 Bolise 
                 SGC-7901 
                 DMEM + 10% FBS 
                 Saturation 
               
               
                   
                   
                   
                   
                 humidity 
               
               
                   
               
            
           
         
       
     
     II. Samples to be Tested 
     Pirubicin targeted drug: DNAh-Biotin-EGFRapt-Cy5-THP; (loaded according to the loading method of the DNA nanoparticles in Embodiment 5). 
     Targeted fluorescent carrier. DNAh-Bio-EGFRapt-Cy5. 
     III. Information about Instruments, Devices and Related Reagents (Same as Above) 
     IV. Experiment Method: 
     1). Adjusting a cell state to a logarithmic growth phase, changing the medium to a medium without biotin and folic acid, and incubating overnight in the incubator at 37 DEG C; 
     2). dissolving the substances to be tested and preparing the to-be-tested substance stock solution; 
     3). digesting and collecting the single-cell suspension and counting, adjusting the cell density to 2×10 5 /mL, planting 1 mL/well into the 24-well plate; 
     4). respectively adding the substances to be tested to the corresponding cell wells, herein the final concentration is 2 μM, shaking and mixing uniformly; 
     5). placing the cell plate in the Incubator at 37 DEG C. and Incubating for 2 hours; 
     6). after the incubation, pancreatin-digesting and collecting the cell suspension; 
     7). centrifugally collecting a cell precipitation, and washing twice with the PBS; 
     8). finally, resuspending the cell precipitation with 300 μL of the PBS, and performing a flow cytometric on-machine detection; herein detection channels of fluorescence carrier or pirarubicin targeted drug: excitation light wavelength: 488 nm, and emission light channel: 560 nm; and 
     9). data analysis. An analysis result is shown in the following table. 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 108 
               
               
                   
                   
               
               
                   
                 Cell line 
                 Test sample 
                 Binding rate (%) 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 SGC-7901 
                 DNAh-Bio-EGFRapt-Cy5-THP 
                 100 
               
               
                   
                   
                 DNAh-Bio-EGFRapt-Cy5 
                 99.99 
               
               
                   
                   
                 Blank control (medium only) 
                 0.34 
               
               
                   
                 SKOV3 
                 DNAh-Bio-EGFRapt-Cy5-THP 
                 99.98 
               
               
                   
                   
                 DNAh-Bio-EGFRapt-Cy5 
                 100 
               
               
                   
                   
                 Blank control (medium only) 
                 0.11 
               
               
                   
                   
               
            
           
         
       
     
     It may be seen from the above table that the DNA nucleic acid nanoparticles carrying the target head and the small molecular drug pirarubicin have a high binding rate to the cells, and it may be apparently seen that it may be bound and internalized with the corresponding tumor cell line cells. In addition, it may also be seen from the above table that the DNAh-Bio-EGFRapt-Cy5-THP may not only efficiently bind and internalize with the human gastric cancer cell line SGC-7901 cells, but also may bind and internalize with the human ovarian cancer cell line SKOV3 cells. It may be seen that the DNAh-Bio-EGFRapt-Cy5-THP, a pirarubicin targeted drug, has both application prospects for the treatment of gastric cancer and ovarian cancer. 
     (IV) Cell Binding Ability of RNAh-Biotin-Cy6-THP Nanoparticles Detected by Flow Cytometer 
     I. Samples to be Tested 
     Targeted drug: RNAh-Biotin-Cy5-THP, herein a preparation method of the RNAh-Biotin-Cy5 is the same as that of the RNAh-Biotin-quasar670, and a difference is that the fluorescent substance is replaced by the Cy5 from the quasar670. The RNAh-Biotin-Cy5-THP is the nanoparticles formed by further loading the THP on the RNAh-Biotin-Cy5 (loaded according to the method in Embodiment 5). 
     II. Experiment Cells and Culture Conditions (MCF-7 Cells, the Details are the Same as the Above Confocal Microscopy Experiment in Embodiment 6, and it is not Repeatedly Described Here) 
     III. Fluorescence Detection 
     Conditions of a fluorescence detection are as follows: 
     Excitation light is 640 nm, emission light is 675 nm, a detection height is 7 mm, measured value/data point-10, detection speed: normal, and extension: 100 ms. 
     IV. Detection Result 
     
       
         
           
               
               
               
               
             
               
                 TABLE 109 
               
             
            
               
                   
               
               
                   
                   
                 Binding rate (%) 
                 Binding rate (%) 
               
               
                 Cell  
                   
                 Treatment time 1 h 
                 Treatment time 2 h 
               
            
           
           
               
               
               
               
               
               
            
               
                 line 
                 Test sample 
                 0.2 μM 
                 0.4 μM 
                 0.2 μM 
                 0.4 μM 
               
               
                   
               
               
                 MCF-7 
                 RNAh-Biotin- 
                 98.69% 
                 99.55% 
                 96.65% 
                 99.23% 
               
               
                   
                 Cy5-THP 
                   
                   
                   
                   
               
               
                   
                 Blank control  
                  0.45% 
                  0.45% 
                  0.24% 
                  0.24% 
               
               
                   
                 (medium only) 
               
               
                   
               
            
           
         
       
     
     It may be seen from the above table that the binding rate of RNAh-Biotin-Cy5-THP nanoparticles and MCF-7 cells may be as high as 96% or more. Compared with the blank control containing the medium only, the RNA drug-loaded particles have a strong ability to bind and internalize with the MCF-7 cells. 
     (V) Cell Binding Ability of RNAh-Biotin-Cy5-Gemcitabine Nanoparticles Detected by Flow Cytometer 
     I. Samples to be Tested 
     Targeted drug: RNAh-Biotin-Cy5-gemcitabine, herein a preparation method of the RNAh-Biotin-Cy5 is the same as that of the RNAh-Biotin-quasar670, and a difference is that the fluorescent substance is replaced by the Cy5 from the quasar670. The RNAh-Biotin-Cy5-gemcitabine is the nanoparticles formed by further loading the gemcitabine on the RNAh-Biotin-Cy5 (loaded according to the method in Embodiment 5). 
     II. Experiment Cells and Culture Conditions (BxPC-3 Cells, Same as Above) 
     III. Fluorescence Detection 
     Conditions of a fluorescence detection are as follows: 
     Excitation light is 640 nm, emission fight is 675 nm, a detection height is 7 mm, measured value/data point=10, detection speed: normal, and extension: 100 ms. 
     IV. Detection Result 
     
       
         
           
               
               
               
               
             
               
                 TABLE 110 
               
             
            
               
                   
               
               
                   
                   
                 Binding rate (%) 
                 Binding rate (%) 
               
               
                   
                   
                 Treatment time 1 h 
                 Treatment time 2 h 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Test sample 
                 0.2 μM 
                 0.4 μM 
                 0.2 μM 
                 0.4 μM 
               
               
                   
               
               
                 BxPC-3 
                 RNAh-Biotin- 
                 95.10% 
                 98.51% 
                 98.17% 
                 99.59% 
               
               
                   
                 Cy5-gemcitabine 
                   
                   
                   
                   
               
               
                   
                 Blank control  
                  0.14% 
                  0.14% 
                  0.16% 
                  0.16% 
               
               
                   
                 (medium only) 
               
               
                   
               
            
           
         
       
     
     It may be seen from the above table that the binding rate of RNAh-Biotin-Cy5-gemcitabine nanoparticles and BxPC-3 cells may be as high as 98% or more in the case that the treatment time and concentration are appropriate. Compared with the blank control containing the medium only, the RNA drug-loaded particles have a strong ability to bind and internalize with the BxPC-3 cells. 
     Embodiment 20 
     Stability Detection of Nanoparticles in Serum 
     (I) Stability of RNAh-Bio-670-EPB Nanoparticles in Serum 
     I. Experiment Material and Experiment Method 
     1. Samples to be tested: RNAh-Bio-670-EPB nanoparticles dissolved in PBS solution. 
     2. Experiment reagents: 
     RPMI-1640 medium (Gibco, C11875500BT-500 mL); Fetal bovine serum (FBS) (ExCell Bio, FNA500-500 mL); Penicillin/Streptomycin (PS) (Gibco, 15140-122-100 mL); PBS buffer solution (Gibco, C20012500BT-500 mL); Novex™ Tris-Glycine Native Sample Buffer (2×) (Invitrogen, LC2673-20 mL); Novex™ 8% Tris-Glycine Mini Gels (Invitrogen, XP00080BOX-1.0 mm); Tris-Glycine Native Running buffer (10×) (Life science, LC2672-500 mL); and G250 staining solution (Beyotime, P0017-250 mL). 
     3. Experiment instrument: 
     Spectrophotometer (Thermo, ND2000C): Mini Gel Tank (Invitrogen, PS0301); and Imaging System (Bio-Rad, ChemiDoc MP). 
     4. Experiment method: 
     (1) Taking 10 μL of the 10 μM RNAh-Bio-670-EPB nanoparticles and placing in 90 μL of a RPMI 1640 medium containing 10% serum and incubating. 
     (2) After being incubated at 37 DEG C. for 10 min, 1 h, 12 h, and 36 h, respectively taking samples. 
     (3) After using NanoDrop for quantification, taking 200 ng of the RNA nanoparticles, adding the same volume of Tris-Glycine SDS sample buffer solution (2×), and adequately mixing uniformly. 
     (4) Taking a piece of Novex™ 8% Tris-Glycine Mini gel, loading the samples in order, setting a program at 200 V, 30 min, and starting electrophoresis. 
     (5) After the electrophoresis is finished, performing G250 staining, placing on a horizontal shaker for 30 min, taking pictures and imaging. 
     II. Experiment Result 
     
       
         
           
               
             
               
                 TABLE 111 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                 RNAh-Bio-  
                 200 ng RNAh- 
                 Buffer  
               
               
                   
                 670-EPB 
                 Bio-670-EPB 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 89.7 
                 2.2 
                 2.2 
               
               
                 10 
                 min 
                 91 6 
                 2.2 
                 2 2 
               
               
                 1 
                 h 
                 89.0 
                 2.2 
                 2.2 
               
               
                 12 
                 h 
                 88.6 
                 2.3 
                 2.3 
               
               
                 36 
                 h 
                 89.4 
                 2.2 
                 2.2 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 64  and  FIG. 65 . Herein,  FIG. 64  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 65  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that 0 min, 10 min, 1 h, 12 h and 36 h, under different time lengths, there is no significant difference between the bands of RNAh-Bio-670-EPB nanoparticles, it is indicated that it is relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     (II) Stability of DNAh-Bio-EGFRapt-Cy5-EPB Nanoparticles in Serum 
     I. Experiment Materials, Reagents and Devices 
     1. Experiment Material 
     DNAh-Bio-EGFRapt-Cy5-EPB nanoparticles 
     2. Experiment Reagent 
     6×DNA loading buffer solution (TSJ010, Geosciences), 100 bp DNA molecular marker (TSJ010, Geosciences); 10000*SolarGelRed nucleic acid dye (E1020, solarbio); 8% non-denaturing polyacrylamide gel (self-made); 1×TBE Buffer (without RNA enzyme) (self-made); serum (FBS) (Excel); and RPMI 1640 (GBICO). 
     PowerPac Basic (Blo-rad), Mini PROTEAN Tetra Cell (Bio-rad), orbital shaker (TS-3D), and Tanon (Tanon 3500). 
     II. Experiment Method 
     (1) Taking 6 μL of the DNAh-Bio-EGFRapt-Cy5-EPB nanoparticles, diluting with 6 μL of the RPMI 1640 medium containing 10% serum, herein the concentration may reach 900 μg/ml after dilution, diluting by 5 tubes respectively, and placing the diluted sample at 37 DEG C. of a water bath for different times (0, 10 min, 1 h, 12 h, and 36 h). 
     (2) Taking the treated sample and mixing with the 6×DNA Loading Buffer, operating on ice, and making a label. 
     (3) Taking 8% Native PAGE, applying a piece of the gel to the nanoparticle samples with the different incubation times, herein a loading amount is 20 μL/well/sample, setting a program at 90-100 V, and performing electrophoresis for 50 min. 
     (4) After the electrophoresis, staining, placing in a horizontal shaker for 30 minutes, taking pictures and imaging. 
     III. Experiment Result 
     
       
         
           
               
             
               
                 TABLE 112 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                 RNAh-Bio-  
                 200 ng RNAh- 
                 Buffer  
               
               
                   
                 670-MTX 
                 Bio-670-MTX 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 95.2 
                 2.10 
                 2.10 
               
               
                 10 
                 min 
                 96.0 
                 2.08 
                 2.08 
               
               
                 1 
                 h 
                 95.3 
                 2.10 
                 2.10 
               
               
                 12 
                 h 
                 96.0 
                 2.08 
                 2.08 
               
               
                 36 
                 h 
                 124.8 
                 1.60 
                 11.80 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 67  and  FIG. 68 . Herein,  FIG. 67  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 68  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that 0 min, 10 min, 1 h, 12 h and 36 h, under different time, lengths, there is no significant difference between the bonds of RNAh-Bio-670-MTX nanoparticles, it is indicated that it is relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     (IV) Stability Detection of Targeted Drug RNAh-Bio-670-THP Nanoparticles in Serum 
     I. Except a sample to be tested: RNAh-Bio-670-THP nanoparticles, the rest are the same as (1). 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 113 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                 RNAh-Bio-  
                 200 ng RNAh- 
                 Buffer 
               
               
                   
                 670-THP 
                 Bio-670-THP 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 95.2 
                 2.10 
                 2.10 
               
               
                 10 
                 min 
                 96.0 
                 2.08 
                 2.08 
               
               
                 1 
                 h 
                 95.3 
                 2.10 
                 2.10 
               
               
                 12 
                 h 
                 96.0 
                 2.08 
                 2.08 
               
               
                 36 
                 h 
                 124.8 
                 1.60 
                 1.60 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 69  and  FIG. 70  Herein,  FIG. 69  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 70  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that 0 min, 10 min, 1 h, 12 h and 36 h, under different time lengths, there is no significant difference between the bands of RNAh-Bio-670-THP nanoparticles, it is indicated that it is relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     (V) Stability Detection of DNAh-Bio-EGFRapt-Cy5-THP Nanoparticles in Serum 
     I. Except a sample to be tested: DNAh-Bio-EGFRapt-Cy5-THP, herein the concentration is 1.8 mg/ml, the rest are the same as (1). 
     II. Experiment result 
     The electrophoresis detection result is shown in  FIG. 71 . Herein, 1 represents 0 min, 2 represents 10 min, 3 represents 1 h, 4 represents 12 h, and 5 represents 36 h. The target band of the DNAh-Bio-EGFRapt-Cy5-THP nanoparticles is about 200 bp. It may be seen from  FIG. 71  that the DNAh-Bio-EGFRapt-Cy5-THP nanoparticles are basically stable after being incubated at 37 DEG C. for 36 h. 
     (VI) Stability Detection of Daunorubicin-Containing Drug Loaded on Nucleic Acid Nanoparticles in Serum 
     I. Except a sample to be tested: RNAh-Bio-670-DNR nanoparticles, the rest are the same as (I). 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 114 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                 RNAh-Bio-  
                 200 ng RNAh- 
                 Buffer 
               
               
                   
                 670-DNR 
                 Bio-670-DNR 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 103.6 
                 1.93 
                 1.93 
               
               
                 10 
                 min 
                 105.6 
                 1.89 
                 1.89 
               
               
                 1 
                 h 
                 103.5 
                 1.93 
                 1.93 
               
               
                 12 
                 h 
                 104.5 
                 1.91 
                 1.91 
               
               
                 36 
                 h 
                 135.2 
                 1.48 
                 1.48 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 72  and  FIG. 73 . Herein.  FIG. 72  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 73  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that 0 min, 10 min, 1 h, 12 h and 36 h, under different time lengths, there is no significant difference between the bands of RNAh-Bio-670-DNR nanoparticles, it is indicated that it is relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     (VII) Stability Detection of Pentafluorouracil-Containing Drug Loaded on Nucleic Acid Nanoparticles in Serum 
     I. Except a sample to be tested: RNAh-Biotin-quasar670-flu nanoparticles, the rest are the same as (I). 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 115 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                 RNAh-Biotin- 
                 200 ng RNAh-Biotin- 
                 Buffer 
               
               
                   
                 quasar670-flu 
                 quasar670-flu 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 104.4 
                 1.9 
                 1.9 
               
               
                 10 
                 min 
                 109.7 
                 1.8 
                 1.8 
               
               
                 1 
                 h 
                 93.4 
                 2.1 
                 2.1 
               
               
                 12 
                 h 
                 101.1 
                 2.0 
                 2.0 
               
               
                 36 
                 h 
                 100.2 
                 2.0 
                 2.0 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 74  and  FIG. 75 . Herein,  FIG. 74  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 75  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that: 0 min, 10 min, 1 h, 12 h and 36 h, under different time lengths, there is no significant difference between the bands of RNAh-Biotin-quasar670-flu nanoparticles, it is indicated that the RNAh-Biotin-quasar670-flu nanoparticles are relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     (VIII) Stability Detection of 10-Hydroxycamptothecin-Containing Drug Loaded on Nucleic Acid Nanoparticles in Serum 
     1. Experiment material and experiment method 
     1. Sample to be tested: RNAh-Biotin-quasar670-hdcp nanoparticles prepared in Embodiment 5. 
     2. Experiment reagent same as (I) 
     3. Experiment instrument: same as (I) 
     4. Experiment method: same as (I) 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 116 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                 RNAh-Biotin- 
                 200 ng RNAh-Biotin- 
                 Buffer 
               
               
                   
                 quasar670-hdep 
                 quasar670-hdep 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 99.4 
                 2.0 
                 2.0 
               
               
                 10 
                 min 
                 96.2 
                 2.1 
                 2.1 
               
               
                 1 
                 h 
                 100.1 
                 2.0 
                 2.0 
               
               
                 12 
                 h 
                 106.6 
                 1.9 
                 1.9 
               
               
                 36 
                 h 
                 109.3 
                 1.8 
                 1.8 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 76  and  FIG. 77 . Herein,  FIG. 76  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 77  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that 0 min, 10 min, 1 h, 12 h and 36 h, under different time lengths, there is no significant difference between the bands of RNAh-Biotin-quasar670-hdcp nanoparticles, it is indicated that the RNAh-Biotin-quaser670-hdcp nanoparticles are relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     (IX) Stability Detection of Aspirin-Containing Drug Loaded on Nucleic Acid Nanoparticles in Serum 
     I. Except a sample to be tested: RNAh-Biotin-quasar670-aspirin nanoparticles, the rest are the same as (D). 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 117 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                 RNAh-Biotin- 
                 200 ng RNAh-Biotin- 
                 Buffer 
               
               
                   
                 quasar670-aspirin 
                 quasar670-aspirin 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 113.6 
                 1.8 
                 1.8 
               
               
                 10 
                 min 
                 114.2 
                 1.8 
                 1.8 
               
               
                 1 
                 h 
                 114.2 
                 1.8 
                 1.8 
               
               
                 12 
                 h 
                 114.0 
                 1.8 
                 1.8 
               
               
                 36 
                 h 
                 117.4 
                 1.7 
                 1.7 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 78  and  FIG. 79 . Herein,  FIG. 78  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 79  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that: 0 min, 10 min, 1 h, 12 h and 38 h, under different time lengths, there is no significant difference between the bands of RNAh-Biotin-quasar670-aspirin nanoparticles, it is indicated that the RNAh-Biotin-quasar67-asprin nanoparticles are relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     (X) Stability Detection of Gemcitabine-Containing Drug Loaded on Nucleic Acid Nanoparticles in Serum 
     1. Except a sample to be tested: RNAh-Bio-670-gemcitabine nanoparticles, the rest are the same as (1). 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 118 
               
             
            
               
                   
               
               
                 quantitative result and loading volume 
               
            
           
           
               
               
               
               
            
               
                   
                 RNAh-Bio- 
                 200 ng RNAh-Bio- 
                 Buffer 
               
               
                   
                 670-gemcitabine 
                 670-gemcitabine 
                 solution 
               
               
                 Sample 
                 (ng/μL) 
                 (μL) 
                 (μL) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 0 
                   
                 109.2 
                 1.8 
                 1.8 
               
               
                 10 
                 min 
                 117.0 
                 1.7 
                 1.7 
               
               
                 1 
                 h 
                 108.4 
                 1.8 
                 1.8 
               
               
                 12 
                 h 
                 122.3 
                 1.6 
                 1.6 
               
               
                 36 
                 h 
                 132.6 
                 1.5 
                 1.5 
               
               
                   
               
            
           
         
       
     
     The electrophoresis detection results are shown in  FIG. 80  and  FIG. 81 . Herein,  FIG. 80  shows the electrophoresis result of 8% non-denaturing gel (Coomassie Blue program), and  FIG. 81  shows the electrophoresis result of 8% non-denaturing gel (Stain Free Gel program). The results of the serum stability test show that: 0 min, 10 min, 1 h, 12 h and 36 h, under different time lengths, there is no significant difference between the bands of RNAh-Bio-670-gemcitabine nanoparticles, it is indicated that it is relatively stable in the 1640 medium with the 10% FBS without significant degradation. 
     Embodiment 21: Toxicity Research of Drug-Loaded Nanoparticles to Cells 
     (I) Cytotoxicity Research of RNAh-Bio-470-EPB Nanoparticles in HL60 Cells 
     I. Experiment Material and Experiment Method 
     1. Experiment Material: 
     Samples to be tested: small molecular drug EPB and RNAh-Bio-670-EPB nanoparticles. 
     Drug Concentration Preparation: 
     Preparing a freshly prepared reagent into a corresponding volume container, and adding PBS to be quantified to 10 μM. 
     Preparing serial dilution solvents with a culture medium, from 10 μM to 3.33 μM, 1.11 μM, 0.370 μM, 0.124 μM, 0.041 μM, 0.014 μM, 0.0046 μM, 0.0015 μM successively. 
     Transferring 50 μl of the solution to each well to obtain the final concentrations of 5 μM, 1.667 μM, 0.556 μM, 0.185 μM, 0.062 μM, 0.021 μM, 0.0069 μM, and 0.0023 μM, respectively. 
     2. Experiment Reagent: 
     Promega; RPMI-1640 medium (Gibco, C11875500BT-500 mL); Fetal bovine serum (FBS) (ExCell Bio, FNA500-500 mL); Penicillin/Streptomycin (PS) (Gibco, 15140-122-100 mL); PBS buffer solution (Gibco, C20012500BT-500 mL); Trypsin-EDTA (Stemcell, 07901-500 mL); DMSO (Sigma, D5879-1 L); and CellTiter-Glo Luminescent Cell Viability Assay kit (CTG) (Promega, G7572-100 mL). 
     3. Experiment instrument 
     Inverted Microscope (Olympus IX71, No. 112A-1); 96-well Plate Reader (Molecular Devices, Flexstation 3); and Perkin Elmer Envision 2104 Multilabel Reader (No. 01-094-0002). 
     4. Experiment Method: 
     1) Cell Culturing and Plating 
     Cells are cultured at 37 DEG C. and 5% CO 2  in a corresponding basal medium in which 10% FBS and 1% PS are respectively added. The cell density used in the experiment is above 80%. The cells are collected, and centrifuged at 1000 rpm for 4 minutes, the medium is resuspended, the cell concentration is adjusted, and it is added to the 96-well plate in a volume of 3000 cells per 50 μL, and each group has 3 replicate wells. 
     2) Gradient Drug Concentration Preparation and Administration 
     After 24 hours, the compound solution is transferred to each well by 50 μL/well. Finally, the solution of which the final concentrations are: 5 μM, 1.667 μM, 0.556 μM, 0.185 μM, 0.062 μM, 0.021 μM, 0.0069 μM, and 0.0023 μM respectively is obtained; 
     Solvent control=DMSO 
     Medium (untreated) control: only cells without compound treatment 
     Blank control: without cos, used for instrument zero calibration 
     3) Cell Culture after Administration 
     The above cells after administration are cultured for 72 hours under a condition of 37 DEG C and 5% CO 2 . 
     4) Cells Treated by Detection Kit 
     The well plate is brought to a room temperature in advance and stands for 30 minutes, 100 μL of a CellTiter-Glo® reagent is added to each well of the well plate and mixed for 2 minutes on a shaker to promote cell lysis. The Perkin Elmer Envision 2104 Multilabel Reader is used to read values and the values are recorded. 
     5) Experiment Data Acquisition and Processing 
     The acquired experiment data is analyzed and processed by using excel software, and GraphPad Prism 5 software is used for curve fitting analysis. 
     II. Experiment Result: 
     
       
         
           
               
             
               
                 TABLE 119 
               
             
            
               
                   
               
               
                 IC 50  value 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Cell 
                 Treatment 
                 Epirubicin (EPB) 
                 RNAh-Bio-670-EPB 
               
               
                   
                 line 
                 time 
                 IC 50 (μM) 
                 IC 50 (μM) 
               
               
                   
                   
               
               
                   
                 HL60 
                 72 h 
                 0.3015 
                 0.06977 
               
               
                   
                   
               
            
           
         
       
     
     The experiment results are shown in Table 119 and  FIG. 82 , it may be seen from Table 119 and  FIG. 82  that the epirubicin (EPB) and RNAh-Bio-670-EPB nanoparticles have a significant inhibitory effect on the proliferation of the HL60 cells, and it is unpredictable that when the concentration is 5 μM, the inhibition rates of the two drugs on the cells are 99.25% and 99.93%, respectively, and when the inhibition rate of cell proliferation is 50%, the IC 50  is 0.06977 μM and 0.3015 μM, respectively. It may be seen that the RNAh-Bio-670-EPB nanoparticles have the stronger inhibitory activity on the cell proliferation, and the drug concentration of the IC 50  thereof is almost ⅕ of the concentration of the small molecular drug EPB, so it may significantly reduce the dosage of the drug and reduce toxic side effects. 
     Furthermore, in order to determine that the targeted fluorescent carrier itself is not significantly toxic to the HL60 cells, the present application further designs a toxicity experiment of the RNAh-Bio-FAM targeted fluorescent carrier to H L60 cells, the toxicity of a small molecular chemical drug Cisplatin to the HL60 cells is used as a control, and a specific result is shown in  FIG. 83  (herein, the highest administration concentration is 10 μM, at this time, the inhibition rate of the RNAh-Bio-FAM targeting fluorescent carrier to the HL60 cells is 8.75%, when the inhibition rate of the control cisplatin to the cells is 99.96%). It may be seen from  FIG. 83  that the fluorescent carrier itself has no apparent toxicity to the HL60 cells. 
     (II) Respective Cytotoxicity of DNAh-Blo-EGFRapt-Cy5-EPB Nanoparticles in MCF-7 and NCI-H1975 Cells 
     1. Experiment Material 
     1. Cell Information: 
     
       
         
           
               
               
               
               
             
               
                 TABLE 120 
               
               
                   
               
               
                 Name 
                 Source 
                 Medium 
                 Culture condition 
               
               
                   
               
             
            
               
                 NCI-H1975 
                 ATCC 
                 RPMI 1640, 
                 37 DEG C., 5% CO 2 , 
               
               
                   
                   
                 10% FBS 
                 saturation humidity 
               
               
                 MCF-7 
                 ATCC 
                 MEM, 10% FBS 
                 37 DEG C., 5%CO 2 , 
               
               
                   
                   
                   
                 saturation humidity 
               
               
                   
               
            
           
         
       
     
     2. Samples to be Tested 
     
       
         
           
               
               
               
               
               
               
               
             
               
                 TABLE 121 
               
               
                   
               
               
                   
                   
                   
                 Drug 
                   
                   
                   
               
               
                   
                 Carrier 
                 Carrier 
                 loaded 
                 Drug 
                   
                   
               
               
                   
                 mass 
                 molecular 
                 mass 
                 molecular 
                   
                 Preservation 
               
               
                 Name 
                 (mg) 
                 weight 
                 (mg) 
                 weight 
                 Character 
                 condition 
               
               
                   
               
             
            
               
                 DNAh-Bio-EGFRapt-Cy5-EPB 
                 0.5 
                 39493 
                 0.190 
                 543.52 
                 Red Solid 
                 −20° C. 
               
               
                 EPB 
                 — 
                 — 
                 2.900 
                 543.52 
                 Red Solid 
                 −20° C. 
               
               
                 DNAh-Bio-EGFRapt-Cy5 
                 1.25*3 
                 39485.9 
                 — 
                 — 
                 Blue Solid 
                 −20° C. 
               
               
                   
               
            
           
         
       
     
     3. Consumables and Devices 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 123 
               
               
                   
                   
               
               
                   
                 Name 
                 Brand 
                 Article number/Model 
               
               
                   
                   
               
             
            
               
                   
                 96-well plate 
                 Corning 
                 3599 
               
               
                   
                 Centrifuge 
                 Gineek 
                 LD5-2B 
               
               
                   
                 CO 2  incubator 
                 Thermo 
                 3111 
               
               
                   
                 Microwell plate shaker 
                 QILINBEIER 
                 QB-9001 
               
               
                   
                 Microscope 
                 Olympus 
                 IX53 
               
               
                   
                 Multifunctional  
                 Bio Tek 
                 Synergy H1 
               
               
                   
                 microplate reader 
               
               
                   
                   
               
            
           
         
       
     
     4. Reagent 
     
       
         
           
               
               
               
               
             
               
                 TABLE 124 
               
               
                   
               
               
                   
                 Name 
                 Brand 
                 Article number 
               
               
                   
               
             
            
               
                   
                 RPMI 1640 medium (without  
                 Provided by 
                 YS3160-500 
               
               
                   
                 folic acid and biotin) 
                 Baiyao Zhidao 
                   
               
               
                   
                   
                 Nano- 
                   
               
               
                   
                   
                 biotechnology 
                   
               
               
                   
                   
                 Co., Ltd 
                   
               
               
                   
                 MEM medium (without 
                 Provided by 
                 YS4150-500 
               
               
                   
                 folic acid and biotin) 
                 Baiyao Zhidao 
                   
               
               
                   
                   
                 Nano- 
                   
               
               
                   
                   
                 biotechnology 
                   
               
               
                   
                   
                 Co., Ltd 
                   
               
               
                   
                 DMEM medium (without  
                 Provided by 
                 YS1200-500 
               
               
                   
                 folic acid and biotin) 
                 Baiyao Zhidao 
                   
               
               
                   
                   
                 Nano- 
                   
               
               
                   
                   
                 biotechnology 
                   
               
               
                   
                   
                 Co., Ltd 
                   
               
               
                   
                 FBS 
                 Cegrogen 
                 A0500-3018 
               
               
                   
                 GlutaMax 
                 Gibco 
                 35050-061 
               
               
                   
                 PBS 
                 Gibco 
                 C14190500BT 
               
               
                   
                 DMSO 
                 Solarbio 
                 D8371 
               
               
                   
                 trypsin-EDTA digestive  
                 Solarbio 
                 T1320-100ml 
               
               
                   
                 juice (0.25%) 
                   
                   
               
               
                   
                 CellTiter 96 ® AQueous  
                 Promega 
                 G3581 
               
               
                   
                 One Solution 
               
               
                   
               
            
           
         
       
     
     II. Experiment Method: 
     1) Harvesting cells in a logarithmic growth phase, taking a small amount and staining with trypan blue for counting to ensure that the cell viability reaches more than 98%; 
     2) adjusting the cell density to 2.22×10 4 /mL with a growth medium: 
     3) planting 90 μL/well of cell suspension into the 96-well plate, herein the number of cells per well in the plate is 2000; 
     4) placing the planted cell plate in the incubator at 37 DEG C. and incubating overnight; 
     5) performing 3.16-fold gradient dilution on the compound at 9 concentration points; 
     6) taking out the cell culture plate, and adding 10 μL/well of 10× concentration drug working solution to the corresponding wells of the cell culture plate, herein three replicate holes are made for each concentration, and the final drug action concentration is shown in the table below. 
     
       
         
           
               
               
             
               
                 TABLE 125 
               
               
                   
               
               
                 Test sample 
                 Concentration gradient 
               
               
                   
               
             
            
               
                 EPB 
                 100 μM, 31.6 μM, 10 μM, 3.16 μM, 1 μM,  
               
               
                   
                 316 nM, 100 nM, 31.6 nM, 10 nM, 
               
               
                   
                 0 (10% PBS) 
               
               
                 DNAh-Bio-EGFRapt- 
                 100 μM, 31.6 μM, 10 μM, 3.16 μM, 1 μM, 
               
               
                 Cy5-EPB 
                 316 nM, 100 nM, 31.6 nM, 10 nM,  
               
               
                   
                 0 (10% PBS) 
               
               
                 DNAh- Bio-EGFRapt- 
                 10 μM, 3.16 μM, 1 μM, 316 nM, 100 nM, 
               
               
                 Cy5 
                 31.6 nM, 10 nM, 3.16 nM, 1 nM,  
               
               
                   
                 0 (10% PBS) 
               
               
                 DMSO 
                 1%, 0.5%, 0.25%, 0.125%, 0.06%, 0.03%, 0 
               
               
                   
               
            
           
         
       
     
     7) placing the cell culture plate in the incubator and continuously incubating for 96 hours; 
     8) placing the CellTiter 96@D AQueous One Solution reagent at the room temperature and thawing for 90 minutes or thawing in a water bath at 37 DEG C., and then equilibrating at the room temperature for 30 minutes; 
     9) adding 20 μL/well of the CellTiter 96® AQueous One Solution reagent to the cell culture plate; 
     10) placing the cell culture plate in the incubator at 37 DEG C. and continuously incubating for 3 hours; 
     11) using the microplate reader to read a OD490 value of each well in the cell plate; and 
     12) data processing and analysis. 
     GraphPad Prism5.0 software is used to process data graphically. In order to calculate the IC50, “W”-shaped non-linear regression analysis is performed on the data to match a suitable dosage-effect curve. A calculation formula of the cell survival rate is as follows, the IC50 may be automatically calculated in the GraphPad Prism 5.0. 
       Cell survival rate (%)=(OD test well −OD blank control )/(OD negative control −OD blank control )×100%.
 
     III. Experiment result (as shown in Table 126,  FIG. 84 , to  FIG. 84 d    and  FIG. 85 a    to  FIG. 85 d   ) 
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE 126 
               
               
                   
                   
               
               
                   
                   
                 Cell line 
                 Test sample 
                 IC 50 (μM) 
               
               
                   
                   
               
             
            
               
                   
                   
                 MCF-7 
                 EPB 
                 0.08473 
               
               
                   
                   
                   
                 DNAh-Bio-EGFRapt-Cy5-EPB 
                 0.04421 
               
               
                   
                   
                   
                 DNAh- Bio-EGFRapt-Cy5 
                 &gt;1 
               
               
                   
                   
                   
                 DMSO 
                 &gt;1% 
               
               
                   
                   
                 NCI-H1975 
                 EPB 
                 0.03062 
               
               
                   
                   
                   
                 DNAh-Bio-EGFRapt-Cy5-EPB 
                 0.01586 
               
               
                   
                   
                   
                 DNAh-Bio-EGFRapt-Cy5 
                 &gt;0.316 
               
               
                   
                   
                   
                 DMSO 
                 &gt;1% 
               
               
                   
                   
               
            
           
         
       
     
     It may be seen from Table 126 and  FIGS. 84 a , 84 b , 84 c  and 84 d    that for the MCF-7 cell line, compared with the simple DNAh targeted fluorescent carrier, the small molecular drug EPB and DNAh drug-loaded particles DNAh-Blo-EGFRapt-Cy5-EPB are both toxic to the MCF-7 cells, and the IC 50  drug concentration of the DNAh drug-loaded particles DNAh-Bio-EGFRapt-Cy5-EPB is a half of the IC 50  drug concentration of the small molecular drug EPB. Similarly, it may seen from Table 126 and  FIGS. 85 a , 85 b , 85 c  and 85 d    that for the NCI-H1975 cell line, compared with the simple DNAh targeted fluorescent carrier, the small molecular drug EPB and DNAh drug-loaded particles DNAh-Bio-EGFRapt-Cy5-EPB are both toxic to the NCI-H1975 cells, and the IC 50  drug concentration of the DNAh drug-loaded particles DNAh-Bio-EGFRapt-Cy5-EPB is a half of the IC 50  drug concentration of the small molecular drug EPB. 
     It may be seen from the above toxicity experiments that the drug-loaded nanoparticles of the present application have a stronger cell proliferation inhibitory effect than the small molecular drugs, and may reduce the dosage of the drug when the same drug effect is achieved, and at the same time reduce toxic side effects. 
     (III) Cytotoxicity Research of RNAh-Bio-670-MTX Nanoparticles in MOLT4 Cells 
     1. Experiment material: 
     Samples to be tested: small molecular drug MTX and RNAh-Bio-70-MTX nanoparticles. 
     2. Experiment method: 
     1) Using the RPMI1640+10% FBS+1% PS medium to culture the MOLT4 cells at 37 DEG C. and 5% CO 2 . 
     2) Collecting the cells, centrifuging at 800 rpm for 5 minutes, resuspending the medium, adjusting the cell concentration, and adding to the 96-we plate in a volume of 5000 cells per 90 μL. 
     3) Diluting a sample to be tested with the culture medium on the next day, respectively adding 200 nM to each sample, herein each sample has 4 replicate wells for replication. 
     4) After being cultured for 72 hours, adding 100 μL of the CTG reagent to each well, shaking for 2 minutes, and standing at the room temperature for 10 minutes, herein a whole process is protected from light. 
     5) Finally using Soft Max Pro5 software to read. 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 127 
               
             
            
               
                   
               
               
                 cell survival rate (%) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Cell  
                 Treatment  
                 Methotrexate  
                 RNAh-Bio- 
               
               
                   
                 line 
                 time 
                 (MTX) 
                 670-MTX 
               
               
                   
                   
               
               
                   
                 MOLT-4 
                 72 h 
                 2.88 
                 4.72 
               
               
                   
                   
               
            
           
         
       
     
     The experiment results are shown in Table 127 and  FIG. 86 . It may be seen from Table 127 and  FIG. 86  that in the MOLT4 cells in vitro, both methotrexate (MTX) and RNAh-Bio-670-MTX nanoparticles have a significant inhibitory effect on the proliferation of the MOLT4 cells, and there is no significant difference between the effects. 
     It is further proved through the toxicity experiments of the fluorescent targeted carrier Bio-Cy5-RNAh and the small molecular chemical drug Cisplatin to the MOLT4 cells that the fluorescent targeted carrier Bio-Cy5-RNAh has no apparent inhibitory effect on the proliferation of the MOLT4 cells (the details are shown in  FIG. 87 ) (Only when the maximum administration concentration is 5 μm, the inhibition rate of the Bio-Cy5-RNAh to the MOLT4 cell proliferation is 47.38%, at this time, the inhibition rate of the control cisplatin to the cell proliferation is 99.94%). 
     (IV) Cytotoxicity of RNAh-Bio-670-THP Nanoparticles in MCF-7 Cells 
     1. Experiment material and experiment method 
     1. Samples to be tested: small molecular drug THP and RNAh-Bio-670-THP nanoparticles; 
     2. Experiment method: 
     1) Except that it is the MCF-7 cells, the rest are the same as (III). 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 128 
               
             
            
               
                   
               
               
                 cell survival rate (%) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Cell  
                 Treatment  
                 Pirarubicin  
                 RNAh-Bio- 
               
               
                   
                 line 
                 time 
                 (THP) 
                 670-THP 
               
               
                   
                   
               
               
                   
                 MCF-7 
                 72 h 
                 13.85 
                 12.93 
               
               
                   
                   
               
            
           
         
       
     
     The experiment results are shown in Table 128 and  FIG. 88 , it may be seen from Table 128 and  FIG. 88  that the RNAh-Bio-670-THP nanoparticles have a significant inhibitory effect on the proliferation of the MCF-7 cells, and the inhibitory effect is slightly stronger than that of the small molecular drug pirarubicin. (THP). 
     Further, in order to determine that the carrier itself is not significantly toxic to the MCF-7 cells, the present application further designs a toxicity experiment of the RNAh-Bio-FAM targeted fluorescent carrier to the MCF-7 cells, the 10% PBS is used as a negative control and the medium is used as a blank control, a specific result is shown in  FIG. 89 . It may be seen from  FIG. 89  that the targeted fluorescent carrier itself has no apparent toxicity to the MCF-7 cells. 
     (V) Cytotoxicity of DNAh-Biotin-EGFRapt-Cy5-THP Nanoparticles in SGC-7901 and SKOV3 Cells 
     I. Experiment Material and Method 
     1. Cell Information 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 129 
               
               
                   
               
               
                   
                   
                 Serial  
                   
                   
               
               
                 Name 
                 Source 
                 number 
                 Medium 
                 Culture condition 
               
               
                   
               
             
            
               
                 SGG-7901 
                 Bolise Co 
                 SGC-7901 
                 DMEM,  
                 37 DEG C., 5% CO 2    
               
               
                   
                   
                   
                 10% FBS 
                 Saturation humidity 
               
               
                 SKOV3 
                 ATCC 
                 HTB-77 
                 MEM,  
                 37 DEG C., 5% CO 2    
               
               
                   
                   
                   
                 10% FBS 
                 Saturation humidity 
               
               
                   
               
            
           
         
       
     
     2. Samples to be Tested 
     
       
         
           
               
               
               
               
               
               
               
             
               
                 TABLE 130 
               
               
                   
               
               
                   
                 Carrier 
                 Carrier 
                 Drug 
                 Drug 
                   
                   
               
               
                   
                 mass 
                 molecular 
                 loaded 
                 molecular 
                   
                 Preservation 
               
               
                 Name 
                 (mg) 
                 weight 
                 mass (mg) 
                 weight 
                 Character 
                 condition 
               
               
                   
               
             
            
               
                 DNAh-Biotin-EGFRapt-Cy5-THP 
                 0.5 
                 39493 
                 0.174 
                 627.64 
                 Red solid 
                 −20° C. 
               
               
                 THP 
                 — 
                 — 
                 0.800 
                 627.64 
                 Red solid 
                 −20° C. 
               
               
                 DNAh-Biotin-EGFRapt-Cy5 
                 1.25*3 
                 39485.9 
                 — 
                 — 
                 Blue solid 
                 −20° C. 
               
               
                   
               
            
           
         
       
     
     II. Experiment Device and Method Same as (II) 
     III. Experiment Result 
     
       
         
           
               
               
               
               
             
               
                   
                 TABLE 131 
               
               
                   
                   
               
               
                   
                 Cell line 
                 Test sample 
                 IC 50 (μM) 
               
               
                   
                   
               
             
            
               
                   
                 SKOV3 
                 THP 
                 0.02374 
               
               
                   
                   
                 DNAh-Bio-EGFRapt-Cy5-THP 
                 0.08462 
               
               
                   
                   
                 DNAh-Bio-EGFRapt-Cy5 
                 &gt;1 
               
               
                   
                   
                 DMSO 
                 &gt;1% 
               
               
                   
                 SGC-7901 
                 THP 
                 0.02739 
               
               
                   
                   
                 DNAh-Bio-EGFRapt-Cy5-THP 
                 0.1195 
               
               
                   
                   
                 DNAh-Bio-EGFRapt-Cy5 
                 &gt;0.316 
               
               
                   
                   
                 DMSO 
                 &gt;1% 
               
               
                   
                   
               
            
           
         
       
     
     It may seen from Table 131 and  FIGS. 90 a , 90 b , 90 c , and 90 d    that for the SKOV3 cell fine, compared with the simple DNAh targeted fluorescent carrier, the small molecular drug THP and DNAh drug-loaded particles DNAh-Bio-EGFRapt-Cy5-THP are both toxic to the SKOV3 cells. Similarly, it may be seen from Table 131 and  FIGS. 91 a , 91 b , 91 c , and 91 d    that for the SGC-7901 cell line, compared with the simple DNAh targeted fluorescent carrier, the small molecular drug THP and DNAh drug-loaded particles DNAh-Bio-EGFRapt-Cy5-THP are both toxic to the SGC-7901 cells. 
     (VI) Cytotoxicity Research of RNAh-Bio-670-DNR Nanoparticles in MCF-7 Cells 
     I. Samples to be tested: small molecular drug DNR and RNAh-Bio-670-DNR nanoparticles; 
     II. Experiment result: 
     
       
         
           
               
             
               
                 TABLE 132 
               
             
            
               
                   
               
               
                 cell survival rate (%) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Cell  
                 Treatment  
                 Daunorubicin  
                 RNAh-Bio- 
               
               
                   
                 line 
                 time 
                 (DNR) 
                 670-DNR 
               
               
                   
                   
               
               
                   
                 MCF-7 
                 72 h 
                 17.33 
                 15.36 
               
               
                   
                   
               
            
           
         
       
     
     The experiment results are shown in Table 132 and  FIG. 92 , it may be seen from Table 132 and  FIG. 92  that the RNAh-Bio-670-DNR nanoparticles have a significant inhibitory effect on the proliferation of the MCF-7 cells, and the inhibitory effect is slightly stronger than that of the small molecular drug daunorubicin. (DNR). 
     Further, in order to determine that the carrier itself is not significantly toxic to the MCF-7 cells, the present application further designs a toxicity experiment of the RNAh-Bio-FAM targeted fluorescent carrier to the MCF-7 cells, the 10% PBS is used as a negative control and the medium is used as a blank control, a specific result is shown in  FIG. 93 . It may be seen from  FIG. 93  that the targeted fluorescent carrier itself has no apparent toxicity to the MCF-7 cells. 
     (VII) Cytotoxicity Research of RNAh-Biotin-Quasar670-Flu Nanoparticles in HepG2 Cells 
     I. Samples to be tested: small molecular pentafluorouracil chemical drug and RNAh-Biotin-quasar670-flu nanoparticles. 
     II. Experiment result 
     
       
         
           
               
             
               
                 TABLE 133 
               
             
            
               
                   
               
               
                 Cell inhibition rate (%) when administration concentration is 5 μM 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 small molecular 
                   
               
               
                 Cell 
                 Treatment 
                 pentafluorouracil 
                 RNAh- Biotin- 
               
               
                 line 
                 time 
                 chemical drug 
                 quasar670-flu 
               
               
                   
               
               
                 HepG2 
                 72 h 
                 39.02% 
                 52.98% 
               
               
                   
               
            
           
         
       
     
     The experiment results are shown in Table 133 and  FIG. 94 , it may be seen from Table 133 and  FIG. 94  that 5 μM of the RNA nanoparticles RNAh-Biotin-quasar670-flu carrying the pentafluorouracil has apparent cytotoxicity to the HepG2 cells, and it is unpredictable that: compared with the inhibitory effect of the small molecular pentafluorouracil drug to the cell proliferation, the inhibition of 5 μM of the RNAh-Biotin-quasar670-flu to the HepG2 cells is more significant, and on the basis that the cell inhibition rate after the treatment of the small molecular pentafluorouracil drug is 39.02%, the inhibition rate thereof to the cells is further improved by at least 25% (improved to 52.98%) 
     In order to further determine that the RNA nanoparticles without carrying the pentafluorouracil have no apparent cytotoxicity to the HepG2 cells, the inventor further designs a toxicity experiment of the RNAh-Biotin-FAM (FAM is a fluorescent marker), a targeted fluorescent carrier, to the HepG2 cells (a drug administration gradient in the experiment is as follows: 100 μM, 31.6 μM, 10 μM, 3.16 μM, 1 μM, 316 nM, 100 nM, 31.6 nM, 10 nM, and 0 (10% PBS)), the results thereof are shown in Table 134 and  FIG. 95 . It may be seen from the IC50 value of Table 134 and  FIG. 95  that the targeted fluorescent carrier without carrying the pentafluorouracil itself has no apparent toxicity to the experimental cells. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 134 
               
               
                   
                   
               
               
                   
                   
                 RNAh-Biotin-FAM 
               
               
                   
                   
               
             
            
               
                   
                 IC 50  (μM) 
                 &gt;10 μM 
               
               
                   
                   
               
            
           
         
       
     
     (VIII) Cytotoxicity Research of RNAh-Biotin-Quasar670-Hdcp Nanoparticles in SGC7901 Cells 
     I. Samples to be Tested: Small Molecular 10-Hydroxycamptothecin Chemical Drug and RNAh-Biotin-Quasar670-Hdcp Nanoparticles. 
     II. Experiment Result 
     
       
         
           
               
             
               
                 TABLE 135 
               
             
            
               
                   
               
               
                 Cell inhibition rate (%) when administration concentration is 5 μM 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 small molecular 10-  
                 RNAh- Biotin- 
               
               
                 Cell 
                 Treatment 
                 hydroxycamptothecin 
                 quasar670-hdep 
               
               
                 line 
                 time 
                 chemical drug 
               
               
                   
               
               
                 SGC7901 
                 72 h 
                 75.43% 
                 94.52% 
               
               
                   
               
            
           
         
       
     
     The experiment results are shown in Table 135 and  FIG. 96 , it may be seen from Table 135 and  FIG. 96  that 5 μM of the RNA nanoparticles RNAh-Biotin-quasar670-hdcp carrying the 10-hydroxycamptothecin has apparent cytotoxicity to the SGC7901 cells, and it is unpredictable that compared with the inhibitory effect of the small molecular 10-hydroxycamptothecin drug to the cell proliferation, the inhibition of 5 μM of the RNAh-Biotin-quasar670-hdcp to the SGC7901 cells is more significant, and on the basis that the cell inhibition rate after the treatment of the small molecular 10-hydroxycamptothecin drug is 75.43%, the inhibition rate thereof to the cells is further improved by at least 25% (improved to 94.52%) 
     In order to further determine that the RNA nanoparticles without carrying the 10-hydroxycamptothecin have no apparent cytotoxicity to the SGC7901 cells, the inventor further designs a toxicity experiment of the RNAh-Biotin-FAM (FAM is a fluorescent marker), a targeted fluorescent carrier, to the SGC7901 cells (a drug administration gradient in the experiment is as follows: 100 μM, 31.6 μM, 10 μM, 3.16 μM, 1 μM, 316 nM, 100 nM, 31.8 nM, 10 nM, and 0 (10% PBS)), the results thereof are shown in Table 136 and  FIG. 97 . It may be seen from the IC 50  value of Table 136 and  FIG. 97  that the targeted fluorescent carrier without carrying the 10-hydroxycamptothecin itself has no apparent toxicity to the experimental cells. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE 136 
               
               
                   
                   
               
               
                   
                   
                 RNAh-Biotin-FAM 
               
               
                   
                   
               
             
            
               
                   
                 IC50 (μM) 
                 &gt;10 μM 
               
               
                   
                   
               
            
           
         
       
     
     (IX) Cytotoxicity Research of RNAh-Biotin-Quasar670-Aspirin Nanoparticles in SH-SY5Y Cells 
     Experiment purpose: an effect of a compound on proliferation of a target tumor cell line is researched. 
     Experiment design: the compound is diluted in 8 concentration gradients and sequentially added to the target tumor cell line and incubated for 72 hours, the CTG kit is used to detect the effect of the compound on the cell proliferation. 
     I. Samples to be tested: RNAh-Biotin-quasar670-aspirin and aspirin 
     II. Experiment result 
     The IC 50  values are shown in Table 137. 
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE 137 
               
               
                   
                   
               
               
                   
                 Cell  
                 Treatment  
                 RNAh-Biotin-quasar670- 
                 Aspirin  
               
               
                   
                 line 
                 time 
                 aspirin IC 50  (μM) 
                 IC 50  (μM) 
               
               
                   
                   
               
             
            
               
                   
                 SH-SY5Y 
                 72 h 
                 0.2744 
                 1430 
               
               
                   
                   
               
            
           
         
       
     
     The specific experiment results are shown in  FIG. 98  and Table 137. It may be seen from  FIG. 98  and Table 137 that the RNA carrier aspirin-loaded chemical group (RNAh-Biotin-quasar670-aspirin) has a significant inhibitory effect on the proliferation of the SH-SY5Y cells; the aspirin chemical group has no apparent inhibitory effect on the SH-SY5Y cells; when the administration concentration is 5 μM, the cell inhibition rates are 98.77% and 17.72% respectively; and the IC 50  is 0.2744 μM and 1430 μM respectively. 
     Furthermore, in order to determine that the targeted fluorescent carrier itself has no apparent toxicity to the SH-SY5Y cells, the present application further designs a toxicity experiment of the RNAh-Bio-Cy5 targeted fluorescent carrier to the SH-SY5Y cells, the toxicity of the small molecule chemical drug cisplatin (Cisplatin) to the SH-SY5Y cells is used as a control, and a specific result is as shown in  FIG. 99  (herein, the highest administration concentration is 10 μM, at this time, the inhibition rate of the RNAh-Bio-Cy5 (also written as Bio-Cy5-RNAh) targeted fluorescent carrier to the SH-SY5Y cells is 29.34%, when the inhibition rate of the control cisplatin is 99.81%). It may be seen from  FIG. 99  that the fluorescent carrier itself has no apparent toxicity to the SH-SY5Y cells. 
     (X) Cytotoxicity Research of RNAh-Bio-670-Gemcitabine Nanoparticles in BXPC3 Cells 
     I. Samples to be tested: small molecular drug gemcitabine and RNAh-Bio-870-gemcitabine nanoparticles 
     II. Experiment result 
     The IC 50  values are shown in Table 138. 
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE 138 
               
               
                   
                   
               
               
                   
                 Cell  
                 Treatment  
                 RNAh-Bio-670- 
                 Gemcitabine  
               
               
                   
                 line 
                 time 
                 gemcitabine IC 50  (μM) 
                 IC 50  (μM) 
               
               
                   
                   
               
             
            
               
                   
                 BXPG3 
                 72 h 
                 0.5916 
                 0.03418 
               
               
                   
                   
               
            
           
         
       
     
     The experiment results are shown in Table 138 and  FIG. 100 , it may be seen from Table 138 and  FIG. 100  that the RNA carrier gemcitabine-loaded histochemical drug (RNAh-Bio-670-gemcitabine) and the gemcitabine chemical drug group both have a significant inhibitory effect on the proliferation of the BxPC3 cells; when the administration concentration is 5 μM, the cell inhibition rates are 99.68% and 82.96% respectively; and the IC 50  is 0.5916 μM and 0.0341 μM respectively. 
     Furthermore, in order to determine that the carrier itself is not significantly toxic to the BXPC3 cells, the present application further designs a toxicity experiment of the RNAh-Bio-FAM targeted fluorescent carrier to the BXPC3 cells, the 10% PBS is used as a negative control, and the medium is used as a blank control, a specific result is shown in  FIG. 101 . It may be seen from  FIG. 101  that the targeted fluorescent carrier itself has no apparent toxicity to the BXPC3 cells. 
     It may be seen from the above description that the above embodiments of the present application achieve the following technical effects: the present application provides a series of nucleic acid nanoparticle carriers with thermodynamic stability, chemical stability, high loading rate and multivalent combination modules. A unique modular design of this type of the carriers is performed to obtain a core modular structure which not only maintains a naturally compatible affinity, but also has high stability and diverse combination characteristics. This structure may flexibly and efficiently integrate various functional modules, including a targeting module, an imaging and probe module, a treatment module and other composite intelligent modules, so that it may be used for targeted delivery in vivo to achieve precise diagnosis and treatment. 
     Through loading the small molecular drugs such as the tacrine on the nucleic acid nanoparticle carrier provided by the present application to form the nucleic acid nanocarrier drug, not only the delivery stability of the drug may be improved, but also in the case that the nucleic acid nanoparticles carry the target head, on the one hand, the drug is targeted and delivered to the target cells, and the bioavailability of the drug is improved; and on the other hand, the toxic and side effects to the non-target cells or tissues are reduced due to the targeted delivery, and the local drug concentration is reduced, thus the toxic and side effects caused by the high drug concentration are further reduced. 
     The above are only the preferred embodiments of the disclosure, and are not used to limit the disclosure, and various modifications and changes may be made to the disclosure by those skilled in the art. Any modifications, equivalent replacements, improvements and the like made within spirit and principle of the disclosure should be included in the scope of protection of the disclosure.