Patent Publication Number: US-5290688-A

Title: Method for intensive, in vitro culture of Babesia divergens strains

Description:
The present invention was made at the Cell Biology Laboratory of POITIERS University, Unite de Recherche Associee au Centre National de la Recherche Scientifique (National Centre for Scientific Research Associated Research Unit), no. 290, and at the Parasitology Laboratory of Rhone Merieux. 
     The present invention relates to a process for the intensive in vitro culture of Babesia divergens and for preparing exoantigens and parasite proteins, and to their use as a vaccine. 
     Babesioses, also known as piroplasmoses, are intraerythrocytic parasitoses which are common in domestic and wild animals (cattle, dogs, horses, rodents, in particular) and rarer in man (16 clinical cases in Europe and approximately 200 in the USA have been described since 1957). They are transmitted by haematophagous acarines (ticks) and, in relevant cases, by blood transfusion in man. 
     There is a large number of Babesia species. 
     Among the various animal babesioses, bovine babesiosis caused by B. bovis (=B. argentina), B. bigemina, B. major and B. divergens is the most important from an economic standpoint. 
     In Europe, bovine babesiosis due to B. divergens causes limited mortality as a result of effective chemotherapy (for example imidocarb). However, cases of chemoresistance are beginning to be observed, especially in France (National Veterinary School, Nantes). On the other hand, the morbidity of the infection is a source of substantial economic losses. 
     In the developing countries, bovine babesiosis represents a major obstacle to increasing the productivity of cattle rearing and one of the main causes of economic losses recorded in some regions of Africa. 
     There are several methods of controling Babesia: 
     control of the arthropod vectors (ticks), 
     curative or preventive antiparasitic chemotherapy, 
     vaccination of animals against Babesia: at the present time, only vaccines against bovine babesiosis due to B. bovis and to B. bigemina (Australia, Latin America, Israel, South Africa) and against canine babesiosis due to B. canis (France) are used on a large scale; there is still no vaccine on the market against B. divergens and B. major, the species responsible for bovine babesiosis in Europe; only Taylor has proposed a vaccine obtained from blood of parasitised cattle (GB-A-2,200,638). Among anti-Babesia vaccines described, there are two major types: live vaccines and inactivated vaccines (their advantages and drawbacks are summarised in Table 1). 
     The parasite exoantigens collected in culture supernatants have already been used for vaccination against bovine babesiosis due to B. bovis and is B. bigemina (EP-0,018,579 B1) and canine babesiosis due to B. canis (EP-0,220,988 A1). 
     
                                           TABLE 1                                 
__________________________________________________________________________
                            Risk of                                       
                                   Risk of                                
                 Ease of                                                  
                       Potential                                          
                            rekindling                                    
                                   erythrocyte                            
                                         Efficacy                         
            Repro-                                                        
                 commercial                                               
                       risk of                                            
                            the virulence                                 
                                   isoimmuni-                             
                                         of the                           
Vaccine Type                                                              
            ducibility                                                    
                 exploitation                                             
                       infection                                          
                            of the strain                                 
                                   sation                                 
                                         vaccine                          
__________________________________________________________________________
LIVE VACCINES:                                                            
a)                                                                        
  &#34;Premunisation&#34;                                                         
            poor no    yes  yes    low   good                             
  (infection followed                                                     
  by chemotherapy)                                                        
b)                                                                        
  Irradiated parasites                                                    
            poor no    ?    ?      low   good                             
c)                                                                        
  Attenuated parasites                                                    
            variable                                                      
                 no    yes  yes    low   good                             
  (rapid passages in                                                      
  animals)                                                                
INACTIVATED VACCINES:                                                     
Prepared from infected                                                    
cattle:                                                                   
a)                                                                        
  Particulate antigens                                                    
            poor no    no   no     high  low                              
b)                                                                        
  Soluble antigens                                                        
            poor no    no   no     high  low                              
  extracted from para-                                                    
  citised red cells                                                       
  lysis supernatants                                                      
            good yes   no   no     low   good                             
c)                                                                        
  soluble plasma                                                          
            poor no    no   no     low   low                              
  antigens                                                                
Prepared from in vitro                                                    
cultures:                                                                 
Soluble exoantigens                                                       
            good yes   no   no     low   good                             
isolated from culture                                                     
supernatants                                                              
__________________________________________________________________________
 
    
     The present invention relates more especially to a process for preparing exoantigens and proteins enabling a vaccine to be obtained against bovine babesiosis due to B. divergens, and which may be extended to other Babesia species. 
     The process for the culture of Babesia is characterised in that the Babesia strain is maintained in culture on a culture medium free from serum protein but containing lipoproteins as well as red cells. 
     The lipoproteins which are usable according to the present invention are preferably of natural origin, in particular of human origin. 
     Among these lipoproteins, high density lipoprotein, hereinafter designated &#34;HDL&#34;, should be mentioned more especially. 
     The indices of multiplication of Babesia divergens in culture in vitro in semi-defined medium are comparable to those obtained with complex media containing from 5 to 10% of human serum. 
     This new technique may be adapted to the in vitro culture of other Babesia species such as B. bovis, B. bigemina, B. canis, B. equi, B. major and B. microti. 
     The use of semi-defined medium enables the exoantigens present in the medium to be purified and has advantages for the culture of Babesia: 
     reduced risk of contamination of the laboratory workers by the possible presence of viruses in the sera (hepatitis virus, HIV); 
     good reproducibility of the growth of the parasites, which is no longer dependent on the variability of serum batches; and 
     elimination of the problems of serum/red cell incompatibility and possibility of using all red cells of the ABO system. 
     This semi-defined medium based on lipoproteins preferably contains HDL fractions at a concentration of between 0.2 and 5 mg in lipid/ml, especially 0.5 and 3 mg in lipid/ml with the addition or otherwise of LDL at a concentration of between 0.5 and 1.5 mg in lipid/ml. This medium can also contain growth factors such as bovine insulin at a concentration of between 0.5 and 10 μg/ml, human transferrin at a concentration of 10 to 200 μg/ml, selenium and betacyclodextrin. 
     These culture media free from serum protein are known. An example of such media is RPMI 1640, used for cell cultures. These media are in general supplemented with serum proteins, in particular with foetal calf serum; in the present case, the supplement consists of lipoproteins. 
     These media are known to those skilled in the art and will not be described further. The terminology of equivalents to RPMI 1640 medium will sometimes be used for these media. 
     This culture process enables Babesia exoantigens, in particular, to be prepared, the exoantigens being isolated from the culture medium. 
     B. divergens produces exoantigens in the culture supernatant, some of which induce immunoprotection in vaccinated animals. The use of semi-defined medium enables parasite antigens which are candidates for the stimulation of humoral and/or cell-mediated immunity to be collected, by eliminating the problems linked to the presence of major plasma proteins (albumin, immunoglobulin) in the culture medium. The process enables a higher concentration of the antigen to be produced while good solubility of the latter is preserved. It likewise improves the purification of the protective serum factors. 
     In the case of B. divergens and B. canis, the collection of antigens hitherto met with the difficulties of maintaining continuous cultures by the methods previously described. Thus, during the culture of B. canis used for the Pirodog vaccine (Rhone Merieux), the parasitaemia level falls below 1% in the course of three weeks (Moreau Y. and Laurent N., In: &#34;Malaria and Babesiosis&#34;, Ristic M., Ambroise-Thomas P., Kreier J. P. (eds), Martinus Nijhoff, Dordrecht, Holland, 1984, 132). This situation hence gave rise to the need to initiate further cultures regularly with red cells of parasitised dogs. 
     The lower levels of parasitaemia, described in the literature, obtained during the culture of B. divergens in bovine red cells and the use of high concentrations of bovine serum do not permit either the accumulation of large amounts of parasite antigens in the culture supernatants or a ready separation of the vaccinating protein fraction; these antigens are present in very small amounts in the supernatant compared with the bovine serum proteins. 
     The B. divergens strain cultured according to the process of the invention, and in particular on human red cells, since June 1987,does not require a daily change of medium for parasitaemia levels below 5%. Since the culture in question is of the continuous type, it is not necessary to initiate cultures regularly with blood of parasitised cattle of gerbils. 
     The B. divergens exoantigens obtained by these culture processes may be purified, concentrated and analysed after labelling with radioactive amino acids. The interference caused by serum proteins during the separation of the parasite proteins can thus be demonstrated. 
     Analysis by gel filtration (Superose 12 column, FPLC, Pharmacia) enables three groups of radiolabelled parasite proteins to be detected in the supernatants originating from semi-defined media, whereas the same experiment carried out in the presence of complete medium (10% serum) shows a single major peak of radioactivity due to an accumulation caused by the presence of serum proteins. 
     The soluble proteins of the B. divergens supernatant which confer protection against infection have the following relative molecular masses: 31-33 kDa (doublet), 35-37 kDa (doublet), 43±2 kDa, 46±3 kDa, 50±2 kDa, 66-68 kDa (doublet), 70±3 kDa, 80±3 kDa, 90±3 kDa, 110±3 kDa, 130±3 kDa, 140±5 kDa and 150 kDa. 
     The present invention relates, in addition, to the fractions of B. divergens exoantigens having a KaV: 
     fraction 1) of between 0 and 0.27, 
     fraction 2) of between 0.27 and 0.57, 
     fraction 3) of between 0.57 and 0.78, 
     fraction 4) of between 0.78 and 0.95, 
     and especially fractions 2 and 4. 
     The invention also relates to the antigenic proteins of which these fractions are composed, and in particular the protein of molecular weight 17,000 ±2,000 Da appearing in fraction 4. 
     This is a protein which is found in both B. divergens and B. canis. It is an exoantigen for which the corresponding antibody has an inhibitory power of 100% at a concentration of 50 μg/ml. This protein is present on the surface of mitochondrial type structures as well as on the membranes. 
     The p35 protein of molecular weight 35,000±2,000 Da is a palmitoylated exoantigen glycoprotein which does not cross with B. canis and which occurs in fractions 3 and 4. 
     The p50 protein of molecular weight 50,000±3,000 Da in fractions F3 and F4 is recognised by the serum of protected animals, cattle and gerbils. 
     The culture technique according to the invention in human red cells or in bovine red cells is usable for an industrial production in semi-automated systems employing large volumes of culture media. The exoantigens originating from the supernatant of these cultures in semi-defined medium are usable for obtaining vaccinating fractions. 
     These fractions may be used mixed with immunity adjuvants, in particular adjuvants of the lipid type and/or saponins, for example. 
     Although it is more especially advantageous to use the fractions described above in the vaccinating strategy, in particular fractions 3 and 4, it is possible to use one or more purified proteins or alternatively proteins obtained by other methods, in particular by genetic recombination. 
     Various antigens can be isolated and used to produce immunosera and/or monoclonal antibodies. 
     These antibody fractions may be used in therapy or alternatively for diagnostic purposes. The monoclonal antibodies may also be used to fractionate the culture supernatants and purify the exoantigens by known processes. 
     The present invention also relates to these various antigen and antibody fractions for use both in therapy and in prevention and/or diagnosis. 
     Other features and advantages of the present invention will become apparent on reading the examples below. 
     In the drawings appended to the present description, 
     FIGS. 1A and 1B show the autoradiograph of the proteins of the HDL (FIG. 1A) and LDL (FIG. 1B) fractions labelled with iodine-125 according to McFarlane (1958); 
     A: in the HDL fraction, the apoproteins A4, E, A1 and A2 are detected, human serum albumin (HSA) is present in variable amounts depending on the preparation of the HDL fraction; 
     B: in the LDL fraction, the apoprotein B-100 is preponderant; 
     FIG. 2A shows the SDS-PAGE (10% acrylamide) electrophoresis of the supernatant of in vitro culture of B. divergens labelled metabolically with [ 35  S]methioninee, 
     1: molecular weight kit 
     2: supernatant of culture in semi-defined medium (H+L) 
     3: supernatant of culture in serum-containing medium (10% human serum); 
     FIG. 2B shows the immunoprecipitation of in vitro culture of [ 35  S]methioninee-labelled B. divergens with an immune human serum, 
     1: molecular weight kit 
     2: supernatant of culture in serum-containing medium (10% human serum) 
     3: supernatant of culture in semi-defined medium (H+L) 
     4: B. divergens total antigens in serum-containing medium (10% human serum) 
     5: B. divergens total antigens in semi-defined medium (H+L); 
     FIGS. 3A and 3B show the separation by gel filtration (Superose 12 column, Pharmacia) of the parasite antigens present in the culture supernatant of [ 35  S]methioninee-labelled B. divergens; 
     FIG. 4 illustrates the immunoprecipitation of B. divergens cultures labelled metabolically with [ 35  S]methioninee. 
     FIG. 5 illustrates the growing of B. divergens in a semi-defined medium containing various concentrations in lipoproteins (HDL or LDL) compared with growing observed in a medium containing 10% of human serum (growing 100%). 
    
    
     EXAMPLE 1 
     Conditions of Continuous Culture of B. divergens in Human Red Cells 
     a) B. divergens strains 
     The B. divergens strain used in the present vaccination strategy is a human strain collected at Rouen in June 1987, and maintained since then in culture in vitro on human red cells. It will be designated B. divergens strain Rouen 1987. Naturally, it is possible to use other strains, this one being mentioned only by way of a specific example. 
     Any bovine strain of B. divergens may be cultured in human red cells by initially preparing a mixed culture comprising bovine red cells/human red cells or gerbil red cells/human red cells. In the latter case, the parasitised gerbil blood originates from gerbils infected either with fresh (or cryopreserved) parasitised bovine blood or, equally well, with fresh (or cryopreserved) parasitised gerbil blood. 
     Several hundred bovine strains collected in France in the regions affected by B. divergens, cryopreserved in liquid nitrogen after prior passages in gerbils, have been gathered at Nantes National Veterinary School (A. Marchand). 
     b) Continuous culture medium 
     Its composition is as follows: 
     
         ______________________________________                                    
RPMI 1640:            10.4   g                                            
NaHCO.sub.3 :         2.1    g/l                                          
HEPES:                8.3    g/l                                          
H.sub.2 O q.s.        900    ml                                           
______________________________________                                    
 
    
     The pH is adjusted to 7.3 (7.2-7.4). 
     100 ml of human serum decomplemented by heating to 56° C. for 30 minutes, and optionally gentamicin (0.1 to 1 mg/l), are then added. 
     DMEM, MEM or any equivalent synthetic medium may be used in place of RPMI 1640. 
     The medium is then filtered through a 0.22 μm filter. The human serum used is a mixture of sera of healthy subjects of different or identical blood groups in the ABO system (serologically virus-negative). 
     Decomplemented bovine sera, selected beforehand for their non-agglutination of 0 +   red cells, may also be used. 
     This is the continuous culture medium (RPMI+10% human serum) in which the human strains isolated at Rouen and at Le Mans have been maintained since June 1987 and May 1988, respectively. 
     c) Red cells 
     The red calls used are human red cells, usually of group O + , but culturing of B. divergens can also be carried out in red cells of any other blood group of the ABO system. In the latter case, while it is preferable to use a compatible serum, the use of incompatible sera is nevertheless possible since agglutination of the red cells does not inhibit the growth of the parasite. 
     The human blood samples, collected under sterile conditions on anticoagulants of the ACD (citric acid, sodium citrate, dextrose) or equivalent type, may be used for the culture for a maximum period of 7 days. Before culturing, the red cells are washed twice in RPMI 1640, then once in RPMI 1640+10% serum+gentamicin. The final centrifugation pellet of washed red cells is used for the culture. 
     d) Culture condition 
     Irrespective of the size of the culture dishes, the haematocrit is between 2 and 5%. Care should be taken, in addition, that the volume of the red cell culture is suited to the area of the culture dish; by way of example, 5 ml of culture having a 2-5% haematocrit for a conventional 25 cm 2  culture dish. 
     Culturing of B. divergens is performed at 37° C.: 
     dish closed after gassing with a ternary gaseous mixture: N 2  : 91%, O 2  : 6%, CO 2  : 3%. 
     dish open in a bell jar: CO 2  : of the order of 5% 
     dish open in a CO 2  incubator: 5%. 
     Starting with a 1% parasitaemia level, these culture conditions enable parasitaemia levels of 30 to 80% to be attained regularly after 3 to 5 days of culture, respectively. The true rate of multiplication per 24 hours is in the region of 5 with strain B. divergens strain Rouen 1987. 
     No change of medium is necessary before the parasitaemia level reaches 5%. A change is performed every 24 hours for parasitaemia levels of between 5 and 30%. Two changes of medium every 24 hours are carried out when the parasitaemia level reaches 30%. The culture supernatants having a parasitaemia level of between 20 and 40% are collected: they form the basis of the proposed vaccine. 
     A subculturing of the human strain (Rouen 1987) of B. divergens every 5 days has enabled it to be maintained in continuous culture since June 1987. 
     EXAMPLE 2 
     In vitro culture of B. divergens in semi-defined media based on lipoproteins and growth factors 
     a) Preparation of the lipoproteins and growth factors 
     The lipoproteins are prepared, starting with human serum from healthy donors, by differential ultracentrifugation according to the technique described by Havel et al., (1955, J. Clin. Exp. Invest., 34, 1345-1353). The density of the serum separated after simple coagulation of the blood without an additive (blood harvested in dry bottles) is adjusted successively to 1.019, 1.063 and 1.210 g/ml by adding KBr in the presence of EDTA (0.1% final). Three successive centrifugations of 22 hours at 120,000 g will enable 3 fractions to be isolated: 
     
         ______________________________________                                    
Very Low Density Lipoprotein (VLDL)                                       
                       1.006-1.019 g/ml                                   
Low Density Lipoprotein (LDL)                                             
                       1.019-1.063 g/ml                                   
High Density Lipoprotein (HDL)                                            
                       1.063-1.210 g/ml                                   
______________________________________                                    
 
    
     The LDL and HDL fractions used for the culture are dialysed at 4° C. for 24 hours, first against saline solution buffered to pH 7.4 with 5 mM Tris-HC1, then against a solution comprising RPMI 1640, 17 mM sodium bicarbonate, 25 MM HEPES (pH 7.4). The solutions obtained are sterilised by filtration through a 0.22#m filter and stored at 4° C. The quantity of lipoproteins is estimated after colorimetric protein assay (Lowry et al. , 1951, J. Biol. Chem., 193, 265-275) multiplying this value by 2 for HDL and by 4 for LDL; it is, in effect accepted that the protein contents of HDL and LDL are 50 and 25%, respectively, of the final mass (Patsch et al., 1974, J. Lip. Res., 15, 356-366). The apoprotein composition of the isolated fractions is monitored by electrophoresis on 15% acrylamide gel (SDS-PAGE). 
     b) Semi-defined medium permitting at least 2 growth cycles 
     The culture medium for the in vitro growth of B. divergens strain Rouen 1987 on human red cells should comprise a mixture of HDL, LDL and growth factors added to RPMI 1640 medium or equivalent media, 25 mM HEPES, 17 mM NaHCO 3  ; the pH is adjusted to 7.4. This base medium may be supplemented by adding antibiotics. 
     The total HDL fraction of human origin (d=1.063-1.210 g/ml) used contains in general the apoproteins AI 28 kDa, AII 17.4 kDa, AIV 45 kDa and C 6.6-8.8 kDa, and serum albumin in an amount which varies according to the preparation of the total HDL fraction. The proteins of the HDL fraction may be labelled specifically with iodine-125 according to the technique of McFarlane (1958, Nature, 182, 53) and detected by SDS-PAGE and autoradiography (FIG. 1A). This HDL fraction is used at concentrations of between 0.5 mg and 2 mg/ml for an initial parasitaemia level of the order of 1%. The apoprotein concentration of the total LDL in the medium is between 0.5 and 1.5 mg/ml, and the above iodine-125 technique according to McFarlane shows a predominant labelling of the apoprotein B-100 (FIG. 1B). 
     Human transferrin and bovine insulin are used at doses of 100 μg/ml (10-200 μg/ml) and 1 μg/ml (0. 5-10 μg/ml) respectively. The index of multiplication, defined as the ratio of the parasitaemia level after reinvasion to the initial parasitaemia level, was determined on the various B. divergens strains cultured in 25cm 2  dishes. The incubations tested related to six stages of reinvasion, which enables it to be ensured that the parasites are still viable at the 6th biological cycle after being set up in culture in semi-defined medium. 
     
                       TABLE 2                                                     
______________________________________                                    
Index of multiplication between the time of setting up                    
in culture (TO) and the 24th hour of culture (T24),                       
and the period T24 to 48 hours (T48)                                      
(H, HDL, L, LDL)                                                          
            INDEX OF                                                      
            MULTIPLICATION                                                
            TO-24      T 24-48                                            
______________________________________                                    
Serum         4            4                                              
HL            4,1          2,8                                            
______________________________________                                    
 
    
     In vitro cultures using HDL and LDL with the addition of 50 μg/ml transferrin, 10 μg/ml selenium and 250 μg/ml beta-cyclodextrin were also prepared. These factors possess properties of stimulating cultures (selenium: peroxidation inhibitor; beta-cyclodextrin: inhibitor of the toxicity of molecules present in the culture medium). Indices of multiplication similar to the above were obtained. 
     For high initial parasitaemia levels (15 to 20%), the indices of multiplication in the HL semi-defined medium are similar to the reference serum-containing medium during the first 20 hours, equivalent to 2 erythrocytic growth cycles of B. divergens (the multiplication cycle of B. divergens cultured in vitro on human red cells is, in effect, of the order of 8 to 10 hours. 
     These culture conditions enable soluble parasite proteins, and in particular exoantigens, to be purified. 
     c) Simplified semi-defined medium for obtaining proteins secreted during a biological cycle 
     The semi-defined medium containing only the total HDL fraction at a concentration of 1 mg in lipid/ml enables the parasite proteins liberated during the different stages of the erythrocytic cycle and at the time of reinvasion to be collected, in particular after metabolic labelling of the parasite using radioactive amino acid(s). 
     This medium hence permits good maturation of B. divergens; it would be possible to collect the antigens produced during this maturation and to concentrate them under favourable conditions characterised by the absence of plasma proteins in the culture medium. 
     EXAMPLE 3 
     Production and purification of B. divergens proteins stimulating humoral immunity and/or cell-mediated immunity 
     a) Metabolic labelling and production of parasite proteins 
     The in vitro cultures of B. divergens on human red cells are incubated with 40 μCi/ml of [ 35  S]-L-methionine (Amersham; more than 1 Ci/μmol) in RPMI 1640 or equivalent medium without methionine, to which the constituents of the semi-defined media are added. Other radioactive amino acids may be used, such as [ 3  H]-L-isoleucine (0.1 Ci/μmol) and [ 3  H]-L-histidine (0.05 Ci/μmol). The culture media containing the parasite proteins secreted into the medium or liberated during reinvasion of the red cells by the merozoites are centrifuged (600 g for 5 min) and then filtered on 0.22 μm Millex filters to remove the particulate constituents. The proteins are then concentrated from 5- to 10-fold by ultrafiltration across membranes (B15 or PM10 Centricon, Amicon). The parasite proteins are analysed by SDS-PAGE electrophoresis followed by autoradiography. 
     b) Separation of the parasite proteins 
     The parasite proteins radiolabelled with one or more amino acids may be fractionated by conventional techniques of liquid chromatography (ion exchange, gel filtration, isoelectric focusing, hydrophobic nature, and the like), combined with a determination of their radioactivity by a counter of the &#34;Flow-one&#34;, model CR (Radiomatic, Tampa USA) type. The purified parasite protein fractions can contain the proteins of the HDL fraction, but are depleted of the many native or modified serum proteins of the host which are liable to interfere with the immune reaction (humoral and/or cell-mediated). 
     c) Identification of the soluble parasite proteins of B. divergens 
     The supernatants of in vitro culture of B. divergens on human red cells in the present of [ 35  S]-L-methionine, concentrated by ultrafiltration, show modifications of electrophoretic migration in complete medium (10% serum) for the proteins from 40 to 70,000 (FIG. 2A). The soluble proteins of the B. divergens culture supernatant which confer protection against infection can be visualised by metabolic labelling with [ 35  S]methionine. They have the following relative molecular masses: 31-33 kDa (doublet), 35-37 kDa (doublet), 43±2 kDa, 46±3 kDa, 50±2 kDa, 66-68 kDa (doublet), 70±3 kDa, 80±3 kDa, 90±3 kDa, 110±3 kDa, 130±3 kDa, 140±5 kDa and 150 kDa. Among the latter, the most immunogenic, visualised by immunoprecipitation in all the isolates tested, have molecular masses varying from 34 to 37 kDa, from 43 to 46 kDa, from 69 to 72 kDa and from 90 to 100 kDa. The interference caused by serum proteins during the separation of the parasite proteins may be confirmed by liquid chromatography. The supernatants of an in vitro culture of B. divergens (8% parasitaemia level), radiolabelled for 12 hours with [ 35  S]-L-methionine, may be separated by gel filtration (Superose 12 column, FPLC, Pharmacia) after filtration (0.22 μm). Three groups of radio labelled parasite proteins are detected in the supernatants originating from semi-defined media with the Flow-one, model CR radioactivity counter (FIG. 3). The same experiment carried out in the presence of complete medium (10% serum) shows a single major peak of radioactivity due to an accumulation caused by the presence of serum proteins (FIG. 3). 
     The antigenic natures of the parasites cultured in normal medium or in semi-defined media are similar, both as regards the total proteins and as regards the soluble proteins of the parasite, as shown by the immunoprecipitation experiments carried out with the same immune serum (FIG. 2B). 
     EXAMPLE 4 
     Identification of the immunogenic proteins of B. divergens from in vitro culture on human red cells, essential to the development of a vaccine, necessitated a study of the immune response to B. divergens in three receptive hosts: ox, man, gerbil. For this study, the following immune sera were used: 
     immune human sera: these were 5 sera originating from French patients, and especially the patient treated successfully at Rouen in June 1987. 
     immune gerbil sera: gerbils were infected experimentally with B. divergens strain Rouen 1987 with 10 8  parsitised red cells/animal, then treated with imidocarb (Carbesia N.D. Lab. Coopers) when the parasitaemia level reached 5% and finally infected with the same strain on several occasions from the following month onwards. The animals thus treated were protected against B. divergens and designated by the name hyperimmune gerbils. 
     immune calf sera: a splenectomised calf (referred to as V1) received successively 10 11  parasitised red cells of the Rouen patient (B. divergens strain Rouen 1987) and then 10 10  red cells of gerbils parasitised by the same strain (the red cells of the cured patient could no longer be used). 
     A splenectomised calf (referred to as V2) received on two occasions 10 10  red cells of gerbils parasitised by the same strain. 
     1) Identification of the immunogenic proteins of B. divergens in lysates of varasitised red cells using immune bovine, gerbil and human sera 
     A metabolic labelling of B. divergens (strain Rouen 1987) cultured in vitro on human red cells was performed according to conventional methods using [ 35  S]methionine with incubation times of 12 hours. The proteins immunoprecipitated by means of the patient&#39;s sera collected from day D4 of the clinical episode up to the 9th month following were analysed by SDS-PAGE electrophoresis in a reducing medium and autoradiographed. 
     Antibodies directed towards the 35 kDa protein group are the first to appear. 
     A communal antigenic identity, in particular with the major protein and minor protein groups, is observed irrespective of the immune serum used. 
     FIG. 4 illustrates the immunoprecipitation of Babesia divergens cultures labelled metabolically ([ 35  S]-methionine) with different immune sera derived from: 
     a: gerbil 
     b: human 
     c: ox 
     (k=molecular weight kit). 
     2. Immunoprecipitations of the parasite proteins resent in B. divergens (strain Rouen 1987) cultured supernatants using three immune sera (human, gerbil, calf) 
     After removal of the serum immunoglobulins by binding to protein A-Staphylococcus or protein A-Sepharose CL 4B (Pharmacia), the culture supernatants were immunoprecipitated using the above three immune sera (human, gerbil, calf). These immunoprecipitations enabled major proteins possessing the same electrophoretic mobility in SDS-PAGE/reducing medium to be demonstrated in the three hosts (30 to 100 kDa), as well as minor proteins possibly possessing a molecular weight above 100 kDa and below 30 kDa. 
     EXAMPLE 5 
     Purification of B. divergens exoantigens 
     The B. divergens Rouen 1987 culture supernatants It (parasitaemia level 30-40%) obtained in semi-defined medium (HDL : 0.5 mg/ml, LDL : 0.125 mg/ml, expressed as mg of the lipoprotein proteins) by the process described above were concentrated 15-fold on Amico CF25 and loaded onto a Superose 12 column (Pharmacia FPLC system). Using 25 mM ammonium acetate buffer, pH 7.3, the various fractions are eluted at a flow rate of 0.3 ml/min. The various fractions obtained are: 
     Fraction I: F1: 6.5 to 11 ml elution volume 
     Fraction II: F2 : 11 to 16.5 ml elution volume 
     Fraction III: F3 : 16.5 to 20 ml elution volume 
     Fraction IV: F4 : 20 to 23 ml elution volume. 
     Table 3 summarises the KaV values of these various fractions, as well as the molecular masses of the antigens contained in each of these fractions, obtained by spreading on SDS-PAGE gel. 
     
                       TABLE 3                                                     
______________________________________                                    
Characteristics of the fractions                                          
       F1      F2        F3        F4                                     
______________________________________                                    
Elution  6.5-11      11-16.5 16.5-20.1                                    
                                     20.1-23                              
Volume (ml)                                                               
KaV         0-0.27 0.27-0.57 0.57-0.78                                    
                                     0.78-0.95                            
MM       136       136       189     189                                  
SDS-PAGE 124       124       101     33                                   
(kDa)    97        101       88      28                                   
         92        90        76      25                                   
         86        88        64      24                                   
         69        84        52      18                                   
         66        72        35      15                                   
         57        64        28                                           
         53        63        26                                           
         49        57        25                                           
         37        49        24                                           
         29        43        22                                           
                   35        20                                           
                   33        18                                           
                   26        16                                           
                   24        14                                           
                   21                                                     
                   16                                                     
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 Column: Superose 12 HR 10/30 (Pharmacia)                                 
 Culture supernatant concentrated 50fold on Centriflo CF25 (Amicon)       
 Kav = (Ve - Vt)/(Vt - Vo)                                                
 Ve: elution volume                                                       
 Vt: column volume                                                        
 Vo: dead volume                                                          
 
    
     EXAMPLE 6 
     Anti-B. divergens vaccine 
     1) Vaccinating antigens 
     Vaccination is carried out using as antigens the parasite exoantigens and proteins collected in the supernatant of in vitro culture of B. divergens on human red cells when the parasitaemia level is of the order of 30 to 40%. The supernatant used as a vaccine corresponds to cultures containing approximately 10 8  parasitised red cells per ml of medium. The culture medium containing the parasite antigens is centrifuged at 1,500 g and then filtered under sterile conditions through a 0.22 μm filter. The dose of vaccinating antigen may be adapted to the animal to be vaccinated by means of the usual processes for concentrating proteins. To the dose of parasite proteins contained in 0.2 ml in the case of the gerbil, a corresponding volume of saponin at a concentration of 1 mg/ml of RPMI 1640 is added as an adjuvant to obtain a final saponin concentration of 0.5 mg/ml. The use of other adjuvants is possible. 
     2) Use of semi-defined culture media for the collection of parasite exoantigens and proteins 
     The semi-defined medium described above makes it possible to collect the parasite exoantigens and proteins with a very low contamination with serum proteins, and possesses a definite advantage compared with earlier patents (for example, the culture medium of Ristic (USA) contains 30 to 50% of serum; that of Canning (GB) 40%). 
     This medium will bring about a substantial improvement in the concentration and fractionation of the parasite antigens, which is necessary for the manufacture of a second-generation vaccine. 
     3) Vaccination of gerbils 
     Gerbils, receptive to infection by B. divergens (Lewis D. and Williams H., 1979, Nature, 278, 170-171) without repeated passages modifying the antigenicity or the virulence of the parasite (Murphy T. M., Gray J. S. et al., 1986, Res. Vet. Science, 40, 285-287), constitute a reference model for testing the efficacy of a vaccine against B. divergens. 
     a) Vaccination protocol 1 
     The vaccine is administered subcutaneously at two points of the back of the gerbils (average weight : 52 g) according to the following protocol: 
     Vaccinated animals: 
     
         ______________________________________                                    
number: 8 4 × 1 dose                                                
                        on D0, D10, D30, D50                              
number: 2 3 × 1 dose                                                
                        on D0, D10, D30                                   
______________________________________                                    
 
    
     Each dose contains 0.2 ml of parasite antigens originating from the concentration of 1.5 ml of culture supernatant from a population of 30%-parasitised human red cells, and contains 0. 2 ml of saponin at a concentration of 1 mg/ml as an adjuvant. 
     Control animals/adjuvant: 4×1 injection 
     number: 2 of 400 μl of saponin on D0, D10, D30, D50 (0.5 mg/ml) 
     Control animals: number: 10 gerbils maintained under the same conditions of nurture as the above animals. 
     b) Vaccination Protocol 2 
     The vaccine is administered subcutaneously at two points of the back of the gerbils (average weight : 60 g) according to the following protocol: 
     vaccinated animals: 
     number: 2 2×1 dose on D0, D20 
     Each dose contains 0.2 ml of parasite antigens originating from the concentration of 1.5 ml of culture supernatant from a population of 30%-parasitised human red cells, and contains 0. 2 ml of saponin at a concentration of 1 mg/ml as an adjuvant. 
     Control animals: 
     number : 2 gerbils maintained under the same conditions of nurture as the above animals. 
     c) Results 
     Immunoprotection against the homologous strain of B. divergens (strain Rouen 1987) (vaccination protocol 1) 
     On D63, the gerbils receive intraperitoneally 5.5×10 4  red cells parasitised by the homologous human strain (Rouen 1987). 
     The 10 unvaccinated gerbils, as well as those which have received only the adjuvant, died 6 to 9 days after contamination. The 10 vaccinated gerbils survive without ever exhibiting the slightest haemoglobinuria (clinical sign of an advanced babesiosis). 
     Immunoprotection against the homologous strain of B. divergens (strain Rouen 1987) (vaccination protocol 2) 
     On D 54, the gerbils receive intraperitoneally 1.6×10 6  red cells parasitised by the homologous human strain (Rouen 1987). 
     The 2 unvaccinated gerbils die 5 days after contamination. The 2 vaccinated gerbils survive without ever exhibiting the slightest haemoglobinuria. 
     Immunoprotection against heterologous bovine strains (protocol 1) 
     The above 8 gerbils which received 4 doses and were protected against the homologous strain (Rouen 1987) were contaminated once more on D79 by the intraperitoneal injection of 2.5×10 7  red cells of gerbils parasitised by heterologous strains, according to the following protocol: 
     
         ______________________________________                                    
B. divergens strains                                                      
                    Vaccinated Control                                    
used for infection  animals    animals                                    
______________________________________                                    
Homologous strain:                                                        
Rouen (Haute Normandie)                                                   
                1987    2          1                                      
Heterologous strains:                                                     
Cote d&#39;Or (Bourgogne)                                                     
                1988    2          1                                      
Indre (Centre)  1988    2          1                                      
Loire-Atlantique                                                          
                1988    2          1                                      
(Pays de Loire)                                                           
______________________________________                                    
 
    
     The four control gerbils die between 78 and 116 hours after contamination. The 8 vaccinated gerbils survive without any haemoglobinuria. 
     EXAMPLE 7 
     Study of the cross-antigenicity between Babesia canis and Babesia divergens 
     a) Indirect immunofluorescence 
     The following anti-B. divergens and anti-B. canis antibodies were used on the B. divergens antigen. 
     gerbil anti-B. divergens serum (1) 
     DG7 (ascites) monoclonal antibody directed towards B. divergens (2) 
     anti-P35 polyclonal antibody directed towards a 35-kDa protein of B. divergens (3) 
     dog anti-B. canis serum (4) 
     monoclonal antibodies directed towards B. canis; 42E3 (5); MA3G1B11 (6) 
     control sera: healthy gerbil serum (7); healthy dog serum (8). 
     (1) and (2) proved positive with a ratio of 1/12,800. 
     (3) and (4) proved positive with a ratio of 1/800. 
     (5), (6), (7) and (8) proved negative with respect to the B. divergens antigen. 
     The following anti-B. divergens and anti-B. canis antibodies were used on the B. canis antigen: 
     dog anti-B. canis serum (1&#39;) 
     MA3G1B11 monoclonal antibody (2&#39;) 
     42E3 monoclonal antibody (3&#39;) 
     DG7 monoclonal antibody (4&#39;) 
     Gerbil anti-B. divergens serum (5&#39;) 
     anti-P35 polyclonal antibody (6&#39;) 
     control sera: healthy gerbil serum (7&#39;); healthy dog serum (8&#39;) 
     The sera or antibodies (1&#39;), (2&#39;), (3&#39;) and (4&#39;) proved positive with a ratio of 1/4,800. 
     (5&#39;) proved positive with a ratio of 1/400. 
     (6&#39;),(7&#39;) and (8&#39;) proved negative on the B. canis antigen. 
     b) Immunoprecipitations 
     The immunoprecipitations performed on [ 35  S]methionine-labelled B. divergens antigens using doganti-B. canis serum and gerbil anti-B. divergens serum demonstrated a large cross-antigenicity between the two species. 
     The major proteins recognised by the two types of immunoserum (dog and gerbil) have the following molecular masses: 
     antigen above 220 kDa 
     doublet of 200 to 210 kDa 
     doublet of 105 to 110 kDa 
     antigen of 85 kDa 
     antigen of 70 kDa 
     antigen of 46 kDa 
     antigen of 37 kDa 
     antigen of 35 kDa 
     EXAMPLE 8 
     Vaccination protocol 
     8.1) Influence of the dose fraction and the number of injections 
     The protocol defined above is modified in the following manner: 
     subcutaneous vaccination 
     injection on D0, D21, D42 
     each dose contains 200 μl of parasite antigens 
     adjuvant: 200 μg of Quil A/injection 
     challenge on D63 by IP injection of 10 6  parasitised red cells 
     The results obtained are collated in Table 4: 
     
                       TABLE 4                                                     
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                      THREE                                               
        TWO INJECTIONS                                                    
                      INJECTIONS                                          
                  reciprocal        reciprocal                            
                  of the            of the                                
          mortal- survival    mortal-                                     
                                    survival                              
TREATMENT ity     time        ity   time                                  
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A         0/10    m =     0     0/8   m =  0                              
(1.5 ml           es.sup.3 =                                              
                          0     0/8   es = 0                              
(equivalent).sup.1                                                        
                  n =     10          n =  8                              
B         1/10    m =     0.017 0/8   m =  0                              
(0.3 ml           es =    0.017       es = 0                              
(equivalent).sup.1                                                        
                  n =     10          n =  8                              
C         10/10   m =     0.192  6/10 m =  0.114                          
(0.06 ml          es =    0.014       es = 0.032                          
(equivalent).sup.1                                                        
                  n =     10          n =  10                             
D         8/10    m =     0.133 8/8   m =  0.203                          
(placebo).sup.2   es =    0.027       es = 0.014                          
                  n =     10          n =  10                             
E         10/10   m =     0.230  7/10 m =  0.121                          
controls          es =    0.08        es = 0.029                          
(physiolo-        n =     10          n =  10                             
gical solution)                                                           
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 .sup.1 ml of crude supernatant contained in a vaccinal dose              
 .sup.2 culture supernatant of healthy red cells                          
 .sup.3 confidence interval of the mean                                   
 
    
     It is seen that the supernatant according to the invention is protective at 1.5 and 0.3 ml equivalent. 
     8.2) Influence of the vaccinating antigenic fraction 
     The procedure is as above, but using various fractions corresponding to fractions 1 to 4 described above, a serum-containing supernatant, that is to say a culture with 10% serum, and a lipo supernatant, that is to say a culture containing lipoproteins. 
     Protection is obtained with all of these fractions. 
     The fractions are collated in Table 5. 
     
                       TABLE 5                                                     
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Results with a virulent challenge                                         
(Gerbil)                                                                  
                         Reciprocal                                       
TREAT-    Mortality      survival time                                    
MENTS     Vaccinated                                                      
                    Placebo  Vaccinated                                   
                                     Placebo                              
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Serum-contain-                                                            
          0/10      10/10    m =  0    m =  0.205                         
ing supernatant              n =  10   n =  10                            
(1.5 ml eq.)                 es = 0    es = 0.017                         
Lipo super-                                                               
          0/10      10/10    m =  0    m =  0.222                         
natant                       n =  10   n =  10                            
(1.5 ml eq.)                 es = 0    es = 0.013                         
Fraction no. 1                                                            
          0/10      10/10    m =  0    m =  0.205                         
(1.5 ml eq.)                 n =  10   n =  10                            
                             es = 0    es = 0.013                         
Fraction no. 2                                                            
          0/9        9/10    m =  0    m =  0.186                         
(1.5 ml eq.)                 n =  9    n =  10                            
                             es = 0    es = 0.025                         
Fraction no. 3                                                            
          0/9        8/10    m =  0    m =  0.176                         
(1.5 ml eq.)                 n =  9    n =  10                            
                             es = 0    es = 0.030                         
Fraction no. 4                                                            
          0/10      10/10    m =  0    m =  0.213                         
(1.5 ml eq.)                 n =  10   n =  10                            
                             es = 0    es = 0.010                         
______________________________________                                    
 
    
     8.3) Heterologous tests 
     Test of heterologous protection: 
     subcutaneous vaccination 
     injections on D0, D21 
     each dose contains 200 μl of parasite antigens (1.5 ml of concentrated culture supernatant) 
     adjustment: 200 μg of Quil A/injection 
     challenge on D42 by IP injection of 10 6  parasitised red cells. 
     
                       TABLE 6                                                     
______________________________________                                    
                 Reciprocal of                                            
                 the survival                                             
Mortality        time                                                     
Strain Vaccine  Placebo  Vaccine   Placebo                                
______________________________________                                    
Rouen  0/10     9/10     m =  0      m =  0.170                           
                         n =  10     n =  10                              
                         s.sup.2 =                                        
                              0      s.sup.2 =                            
                                          0.0046                          
German 4/10     10/10    m =  0.069  m =  0.21                            
                         n =  10     n =  10                              
                         s.sup.2 =                                        
                              0.0884 s.sup.2 =                            
                                          0.0025                          
English                                                                   
       2/10     10/10    m =  0.045  m =  0.196                           
                         n =  10     n =  10                              
                         s.sup.2 =                                        
                              0.0091 s.sup.2 =                            
                                          0.0014                          
7107A  0/10     8/10     m =  0      m =  0.129                           
                         n =  10     n =  10                              
                         s.sup.2 =                                        
                              0      s.sup.2 =                            
                                          0.0062                          
Irish  0/10     0/10     m =  0      m =  0                               
                         n =  10     n =  10                              
                         s.sup.2 =                                        
                              0      s.sup.2 =                            
                                          0                               
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     A protection is seen for heterologous strains, although the results remain average for the so-called &#34;German&#34; strain.