Patent Publication Number: US-2016222068-A1

Title: Constructs for expressing biological molecules that integrate into bacterial microcompartments

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is divisional application of U.S. patent application Ser. No. 13/564,676, which is a continuation-in-part application of International Patent Application No. PCT/US2011/023416, filed on Feb. 1, 2011, which claims priority to U.S. Provisional Application No. 61/300,338, filed on Feb. 1, 2010, all of which are hereby incorporated by reference in their entirety. This application is related to and incorporates by reference U.S. patent application Ser. No. 13/367,260, filed on Feb. 6, 2012 in its entirety for all purposes. 
    
    
     STATEMENT OF GOVERNMENTAL SUPPORT 
     This invention was made with government support under Contract No. DE-AC02-05CH11231 awarded by the U.S. Department of Energy. The government has certain rights in the invention. 
    
    
     REFERENCE TO SEQUENCE LISTING AND TABLES 
     This application also incorporates by reference the attached sequence listings which is also found in computer-readable form in a *.txt file entitled, “2785US_sequencelisting_asfiled_ST25.txt”, created on Apr. 20, 2016. 
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates to synthetic biology, especially using targeting signals for integrating biomolecules and molecules into bacterial microcompartments or for attaching molecules or biomolecules to bacterial microcompartment shell proteins. 
     2. Related Art 
     Bacterial microcompartments (BMCs) encapsulate functionally related reactions. BMC shell proteins and the components they encapsulate are typically found in gene clusters (putative operons). The shells of BMCs are generally comprised of multiple paralogs of proteins containing the BMC domain (e.g., Pfam 00936) and presumably a relatively small number of proteins containing the Pfam03319 domain. There is recognizable sequence homology among the &gt;2000 BMC domain-containing proteins now in the sequence databases, suggesting that despite functional diversity and some differences in the morphology of a specific BMC type, there are conserved structural determinants for targeting and binding of the enzymes and auxiliary proteins that are encapsulated in BMCs. 
     Carboxysomes are the foremost example of the polyhedral subcellular inclusions that have been termed bacterial microcompartments, self-assembling protein shells that encapsulate enzymes and other functionally related proteins. In addition to carboxysomes, two other types of bacterial microcompartments (BMCs) are relatively well characterized by others; they function in propane-diol utilization (encoded by the pdu operon) and ethanolamine utilization (encoded by the eut operon) in heterotrophic bacteria. Carboxysomes have been observed in all cyanobacteria and in many chemoautotrophs. 
     BRIEF SUMMARY OF THE INVENTION 
     The present invention describes a common motif (peptide) found in a subset of proteins presumed to be encapsulated in functionally diverse bacterial microcompartments (BMCs). This common motif and adjacent linker region were identified as important for targeting proteins to BMCs. All BMC targeting peptides share general properties such as a region predicted to have an alpha helical conformation, adjacent to poorly conserved segment(s) of primary structure enriched in proline and glycine; for each type of encapsulated protein, for each functionally distinct BMC. Amino acid properties are conserved in many of the positions within these peptides. We have also identified a consensus amino acid sequence for the targeting peptide specific to various BMC types. 
     The present invention also provides for an isolated polypeptide comprising a sequence selected from SEQ ID NOS: 1-349 or a fragment thereof. An expression cassette comprising a polynucleotide encoding a peptide selected from SEQ ID NOS: 1-349 or a fragment thereof can be made. The expression cassette further comprising a cluster of microcompartment genes isolated from a bacteria, wherein the cluster comprising a set of microcompartment genes necessary for the expression of a microcompartment. 
     The expression cassette can be used to provide a cell comprising in its genome at least one stably incorporated expression cassette, where the expression cassette comprising a heterologous nucleotide sequence of any of SEQ ID NOS: 1-349 or a fragment thereof operably linked to a promoter that drives expression in the cell. 
     Also provided are methods for enhancing metabolic activity in an organism. In one method, comprising introducing into an organism at least one expression cassette operably linked to a promoter that drives expression in the organism, where the expression cassette comprising a cluster of microcompartment genes isolated from a bacteria, wherein the cluster comprising a set microcompartment genes necessary for the expression of a microcompartment that has metabolic, wherein the microcompartment genes further comprise a polynucleotide expressing a peptide of SEQ ID NOS: 1-349 or a fragment thereof. 
     BRIEF DESCRIPTION OF THE SEQUENCES 
     SEQ ID NOS: 1-22 are actual localization peptide sequences from proxy organisms and shown in Table 2. 
     SEQ ID NOS: 23-44 are consensus peptide sequences for specific BMC-associated pathway enzymes and proteins as shown in Table 3. 
     SEQ ID NO: 45 is the consensus peptide motif as described in  FIG. 3C . 
     SEQ ID NO: 46 is a consensus peptide sequence derived from the conserved C-termini in carboxysomal protein, CcmN, in  Synechocystis  sp. PCC6803 and  Synechococcus elongatus  PCC7942. 
     SEQ ID NOS: 47-69 are peptide sequences obtained from GenBank for organisms listed in  FIGS. 1, 2   a , and  2   b.    
     SEQ ID NOS: 70-82 are peptide sequences obtained from GenBank for organisms listed in  FIG. 5   a.    
     SEQ ID NOS: 83-94 are peptide sequences obtained from GenBank for organisms listed in  FIG. 6 . 
     SEQ ID NOS: 95-117 are peptide sequences obtained from GenBank for organisms listed in  FIG. 8 . 
     SEQ ID NOS: 118-129 are peptide sequences obtained from GenBank for organisms listed in  FIG. 10 . 
     SEQ ID NOS: 130-144 are peptide sequences obtained from GenBank for organisms listed in  FIG. 11   a.    
     SEQ ID NOS: 145-190 peptide sequences used for helical wheel projection of the predicted alpha helix of various regions of CcmN in various organisms from  FIGS. 4 a , 4 b , 5 b , 5 c , 7 a , 7 b , 9 a  9 b      12 - 24 . 
     SEQ ID NOS: 191-193 are various parts of the CcmN protein sequences used in transformation in Examples 2 and 3. 
     SEQ ID NOS: 194-205 are peptide sequences of the N-terminal region of a pdu-associated aldehyde dehydrogenase (PduP) from 12 microorganisms from  FIG. 3   a.    
     SEQ ID NOS: 206-228 are sequences for CcmN protein of various cyanobacteria from  FIG. 1 . 
     SEQ ID NOS: 229-251 are peptide sequences of the conserved N-terminal domain and variable regions of the CcmN protein of various organisms from  FIG. 2   a.    
     SEQ ID NOS: 252-274 are peptide sequences for the targeting peptide region of the CcmN protein of various organisms from  FIG. 2   b.    
     SEQ ID NOS: 275-287  FIG. 5 a    are peptide sequences for the N-terminal region of Diol dehydratase medium subunit (PduD) from 13 microorganisms from  FIG. 5   a.    
     SEQ ID NOS: 288-299 are peptide sequences for the N-terminal region of diol dehydratase small subunit (PduE) from 11 microorganisms from  FIG. 6 . 
     SEQ ID NOS: 300-322 are peptide sequences for the N-terminal region of the EutC (Ammonia lyase light chain) N-terminal region from 23 microorganisms from  FIG. 8 . 
     SEQ ID NOS: 323-334 are peptide sequences for B12-independent diol dehydratase interdomain peptide of various organisms from  FIG. 10 . 
     SEQ ID NOS: 335-349 are peptide sequences for L-Fuculose phosphate aldolase C-terminal region of various organisms from  FIG. 11 a      
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS AND TABLES 
         FIG. 1  is an alignment of the primary structure of CcmN, a protein encapsulated in the carboxysome, from various cyanobacteria with secondary structure prediction. SEQ ID NO: 206 is  Synechococcus _sp._JA-3-3Ab, SEQ ID NO: 207 is  Synechococcus _sp._JA-2-3B′a(2-13), SEQ ID NO: 208 is  Trichodesmium _ erythraeum,  SEQ ID NO: 209 is  Synechococcus _sp_PCC7002, SEQ ID NO: 210 is  Cyanothece _sp_PCC8801, SEQ ID NO: 211 is  Cyanothece _sp_PCC8802, SEQ ID NO: 212 is  Crocosphaera _ watsonii,  SEQ ID NO: 213 is  Cyanothece _sp_CCY0110, SEQ ID NO: 214 is  Cyanothece _sp_ATCC51142, SEQ ID NO: 215 is  Acaryochloris _ marina _MBIC11017, SEQ ID NO: 216 is  Cynotece _sp_PCC7822, SEQ ID NO: 217 is  Microcystis _ aeruginosa,  SEQ ID NO: 218 is  Synechocytis _sp_PCC6803, SEQ ID NO: 219 is  Gloeobacter _ violaceus,  SEQ ID NO: 220 is  Lyngbya _sp_PCC8106, SEQ ID NO: 221 is  Nostoc _sp._PCC7120, SEQ ID NO: 222 is  Anabaena _ variabilis,  SEQ ID NO: 223 is  Nodularia _ spumigena,  SEQ ID NO: 224 is  Nostoc _ punctiforme,  SEQ ID NO: 225 is  Cyanothece _sp_PCC7425, SEQ ID NO: 226 is  Thermosynechococcus _ elongatus,  SEQ ID NO: 227 is  Synechococcus _ elongatus _PCC6301 and SEQ ID NO: 228 is  Synechococcus _ elongatus _PCC7942. 
         FIGS. 2 a  and 2 b    are close-ups of the alignment and secondary structure prediction of the C-terminal region of the CcmN protein in various organisms.  FIG. 2A  shows the CcmN, C-terminal alignment and secondary structure predictions of the conserved N-terminal domain and variable regions of various organisms. SEQ ID NO: 229 is  Synechococcus _sp._JA-3-3Ab, SEQ ID NO: 230 is Synechococcus_sp._JA-2-3B′a(2-13), SEQ ID NO: 231 is  Trichodesmium _ erythraeum _IMS101, SEQ ID NO: 232 is  Synechococcus _sp. 7002, SEQ ID NO: 233 is  Cyanothece _sp._PCC8801, SEQ ID NO: 234 is  Cyanothece _sp._PCC8802, SEQ ID NO: 235 is  Crocosphaera _ watsonii _WH8501, SEQ ID NO: 236 is  Cyanothece _sp._CCY0110, SEQ ID NO: 237 is  Cyanothece _sp._ATCC51142, SEQ ID NO: 238 is  Acaryochloris _ marina _MBIC11017, SEQ ID NO: 239 is  Cynotece _sp._PCC7822, SEQ ID NO: 240 is  Microcystis _ aeruginosa,  SEQ ID NO: 241 is  Synechocytis _sp._PCC6803, SEQ ID NO: 242 is  Gloeobacter _violaceus, SEQ ID NO: 243 is  Lyngbya _sp._PCC8106, SEQ ID NO: 244 is  Nostoc _sp._PCC7120, SEQ ID NO: 245 is  Anabaena _ variabilis _ATCC29413, SEQ ID NO: 246 is  Nodularia _ spumigena, SEQ ID NO:  247 is  Nostoc _ punctiform,  SEQ ID NO: 248 is  Cyanothece _sp._PCC7425, SEQ ID NO: 249 is  Thermosynechococcus _ elongatus  BP1, SEQ ID NO: 250 is  Synechococcus _ elongatus _PCC6301 and SEQ ID NO: 251 is  Synechococcus _ elongatus _PCC7942.  FIG. 2B  shows the CcmN, C-terminal alignment and secondary structure prediction of the targeting peptide region of the CcmN protein. SEQ ID NOs: 252-274 correspond to the targeting peptide region of the CcmN protein from  Acaryochloris marina  MBIC11017 (SEQ ID NO: 252),  Trichodesmium erythraeum  (SEQ ID NO: 253),  Synechococcus elongatus  PCC 6301 (SEQ ID NO: 254),  Synechococcus elongatus  PCC 794 (SEQ ID NO: 255),  Gloeobacter violaceus  (SEQ ID NO: 256),  Synechococcus  sp. JA-3-3Ab (SEQ ID NO: 257),  Synechococcus  sp. JA-2-3B′a(2-13) (SEQ ID NO: 258),  Nodularia spumigena  (SEQ ID NO: 259),  Nostoc punctiforme  (SEQ ID NO: 260),  Anabaena variabilis  (SEQ ID NO: 261),  Nostoc  sp PCC 7120 (SEQ ID NO: 262),  Lyngbya  sp PCC 8106 (SEQ ID NO: 263),  Synechococcus  sp PCC7002 (SEQ ID NO: 264),  Microcystis aeruginosa  (SEQ ID NO: 265),  Cyanothece  sp PCC8801 (SEQ ID NO: 266),  Cyanothece  sp PCC8802 (SEQ ID NO: 267),  Cyanothece  sp CCY0110 (SEQ ID NO: 268),  Cyanothece  sp ATCC51142 (SEQ ID NO: 269),  Crocosphaera watsonii  (SEQ ID NO: 270),  Synechocystis  sp PCC 6803 (SEQ ID NO: 271),  Cyanothece  sp PCC 7822 (SEQ ID NO: 272),  Thermosynechococcus elongatus  (SEQ ID NO: 273) and  Cyanothece  sp PCC 7425 (SEQ ID NO: 274). 
         FIG. 3A  shows the alignment and secondary structure prediction of the N-terminal region of a pdu-associated aldehyde dehydrogenase (PduP) from 12 microorganisms including an ortholog of PduP (from  Propionibacterium acnes ) that is not associated with bacterial microcompartments and does not contain a targeting peptide. The N-terminal peptide of the Salmonella typhimurium LT2 PduP has been shown to target a pdu-type bacterial microcompartment in Fan et al. 2010. The helical wheel representation for this peptide is shown in  FIG. 3C (II). The first sequence of the alignment is an ortholog of PduP that is not associated with bacterial microcompartments and therefore does not contain a targeting peptide. SEQ ID NOs: 194-205 correspond to  Propionibacterium acnes  J139 (SEQ ID NO: 194),  Fusobacterium ulcerans  ATCC 49185 (SEQ ID NO: 195),  Escherichia coli  CFT073 (SEQ ID NO: 196),  Pectobacterium wasabiae  WPP163 (SEQ ID NO: 197),  Listeria monocytogenes  104035 (SEQ ID NO: 198),  Shewanella  sp W3-18-1 (SEQ ID NO: 199),  Tolumonas aurensis  DSM 9187(SEQ ID NO: 200),  Yersinia frederiksenii  ATCC 33641 (SEQ ID NO: 201),  Klebsiella pneumoniae  342 (SEQ ID NO: 202),  Salmonella typhimurium  LT2 (SEQ ID NO: 203),  Salmonella enterica  Paratyphi B str. Sp87 (SEQ ID NO: 204) and  Citrobacter koseri  ATCC BAA 895 (SEQ ID NO: 205). 
         FIG. 3B  shows an alignment overview of all BMC targeting peptides (305 unique sequences of N- and C-terminal and inter-domain peptides). All unique BMC targeting peptides are colored based on amino acid property with positional amino acid variations indicated as percentages and consensus amino acid properties at each position indicated. The position of the consensus predicted helix is indicated by the thick, black bar under residues 3-13. 
       Helical wheel representations of the targeting peptides in various organisms are shown in the figures. In the helical wheel representations of the predicted alpha helix on the left panel represents the predicted helical targeting peptide for the organism protein listed. Hydrophobic residues are represented as diamonds where the color scale is from dark gray, for most hydrophobic, with amount of gray decreasing proportionally to the hydrophobicity, to light gray. Hydrophilic residues are represented as circles where the color scale is from black, for most hydrophilic, with amount of black decreasing proportionally to the hydrophilicity, to light gray. Potential negatively charged residues are represented as triangles colored light gray. Potential positively charged residues are represented as pentagons colored light gray. 
       In the helical wheel representations shown in the figures, the alpha helix on the right panel of each figure represents the portion of the predicted helical targeting peptide for the organism as mapped onto the consensus helical wheel prediction for all targeting peptides shown in  FIG. 3C  and using the scheme shown in  FIG. 3D . Hydrophobic residues are represented as diamonds. Hydrophilic residues are represented as circles where light gray shading represents polar uncharged residues and dark gray shading represents positively or negatively charged residues. In the consensus helical wheel representations, positions with variable amino acid composition are denoted with a triangle. 
         FIG. 3C  shows the consensus peptide motif Majority amino acid percentages at each well-aligned position were calculated in Jalview. Amino acid property at each position was given based on the majority amino acid property (H=hydrophobic, C=charged, P=polar) at each aligned position. Positions 5 and 15 were highly variable based on identity and property and no consensus property denoted by an X. 
         FIG. 3D  describes mapping of consensus residues and known PduP targeting sequence onto consensus helix prediction. Panel I shows a portion of the consensus sequence of the CcmN C-terminal peptide mapped onto a helical wheel diagram based on a consensus helix prediction for all BMC targeting peptides. Panel II shows a portion of the known targeting peptide sequence from PduP (Fan et al. 2010) mapped onto the consensus helix and the consensus amino acid property at each position based on the alignment of all BMC targeting peptides ( FIG. 3B ) mapped on the consensus helix. The numbering is based on the 17 well-aligned residues shown in the motif in  FIG. 3C . Panel III shows the consensus helix based on properties of all aligned targeting sequences. 
         FIGS. 4A  and B show helical wheel projection of the predicted alpha helix of the C-terminal region of CcmN of  Synechococcus elongatus  PCC7942 (SEQ ID NO: 145 and 146) and  Synechocystis  PCC 6803 (SEQ ID NO: 147-148). 
         FIG. 5A  shows the alignment and secondary structure prediction of the N-terminal region of Diol dehydratase medium subunit (PduD) from 13 microorganisms. SEQ ID NOs: 275-287 correspond to  Lactobacillus brevis  (SEQ ID NO: 275),  Desulfatibacillum alkenivorans  (SEQ ID NO: 276),  Sebaldella termitidis  (SEQ ID NO: 277),  Thermoanaerobacter  sp. X514 (SEQ ID NO: 278),  Thermosediminibacter oceani  (SEQ ID NO: 279),  Dethiosulfovibrio peptidovorans  (SEQ ID NO: 280),  Yersinia bercovieri  (SEQ ID NO: 281),  Klebsiella pneumoniae  (SEQ ID NO: 282),  Shigella sonnei  (SEQ ID NO: 283),  Escherichia coli  (SEQ ID NO: 284),  Citrobacter koseri  (SEQ ID NO: 285),  Salmonella typhimurium  (SEQ ID NO: 286) and  Salmonella enterica  (SEQ ID NO: 287).  FIGS. 5B and 5C  shows helical wheel projections of a peptide from the diol dehydratase medium subunit (PduD) N-terminal region in  Salmonella typhimurium  (SEQ ID NO: 149-152) and  Lactobacillus brevis  (SEQ ID NO: 153-154). The peptides shown fall within the protein sequences are shown boxed in  FIG. 5A . The peptides on the right panels in  FIGS. 5B and 5C  are helical wheel projections of the portion of the predicted peptide that map onto the consensus helical wheel prediction for all peptides. 
         FIG. 6  shows the alignment and secondary structure prediction of the N-terminal region of diol dehydratase small subunit (PduE) from 11 microorganisms. SEQ ID NOs: 288-299 correspond to:  Lactobacillus brevis  (SEQ ID NO: 288),  Sebaldella termitidis  (SEQ ID NO: 289),  Dethiosulfovibrio peptidovorans  (SEQ ID NO: 290),  Thermoanaerobacter  sp. X514 (SEQ ID NO: 291),  Thermosediminibacter oceani  (SEQ ID NO: 292),  Yersinia bercovieri  (SEQ ID NO: 293),  Klebsiella pneumoniae  (SEQ ID NO: 294),  Shigella sonnei  (SEQ ID NO: 295),  Escherichia coli  (SEQ ID NO: 296),  Salmonella enterica  (SEQ ID NO: 297),  Salmonella typhimurium  (SEQ ID NO: 298) and  Citrobacter koseri  (SEQ ID NO: 299). 
         FIGS. 7A and 7B  shows the helical wheel projections of the N-terminal region (boxed in  FIG. 6 ) from the diol dehydratase small subunit (PduE) in  S. typhimurium  (SEQ ID NO: 155-156),  S. termitidis  (SEQ ID NO: 157-158) and  L. brevis  (SEQ ID NO: 159 and 160) on the left hand side of the figures. The region of the peptides within the protein sequences are shown boxed in  FIG. 6 . The peptides on the right panels in  FIGS. 7A and 7B  are helical wheel projections of the portion of the predicted peptide that map onto the consensus helical wheel prediction for all peptides. 
         FIG. 8  shows the alignment and secondary structure prediction of the N-terminal region of the EutC (Ammonia lyase light chain) N-terminal region from 23 microorganisms. SEQ ID NOs : 300-322 corresponds to:  Bacillus  sp. B14905 (SEQ ID NO: 300),  Nocardioides  sp. JS614 (SEQ ID NO: 301),  Alkaliphilus metalliredigens  QYMF (SEQ ID NO: 302),  Leptotrichia buccalis  C-1013-b (SEQ ID NO: 303),  Sebaldella termitidis  ATCC 33386 (SEQ ID NO: 304),  Fusobacterium nucleatum  ATCC 25586 (SEQ ID NO: 305),  Bacteroides capillosus  ATCC 29799 (SEQ ID NO: 306),  Clostridium phytofermentans  ISDg (SEQ ID NO: 307),  Streptococcus sanguinis  SK36 (SEQ ID NO: 308),  Thermanaerovibrio acidaminovorans  Su883 (SEQ ID NO: 309),  Enterococcus faecalis  V583 (SEQ ID NO: 310),  Alkaliphilus oremlandii  OhILAs (SEQ ID NO: 311),  Clostridium difficile  630 (SEQ ID NO: 312),  Listeria monocytogenes  10403S (SEQ ID NO: 313),  Marinobacter aquaeolei  VT8 (SEQ ID NO: 314),  Yersinia intermedia  ATCC 29909 (SEQ ID NO: 315),  Klebsiella pneumoniae  (SEQ ID NO: 316),  Citrobacter koseri  (SEQ ID NO: 317),  Escherichia coli  HS (SEQ ID NO: 318),  Salmonella Typhimurium  LT2 (SEQ ID NO: 319),  Salmonella enterica  Paratyphi A ATCC 9150 (SEQ ID NO: 320),  Photobacterium profundum  3TCK (SEQ ID NO: 321) and  Shewanella benthica  KT99 (SEQ ID NO: 322). 
         FIGS. 9A and 9B  shows the helical wheel projections of targeting peptides from the EutC N-terminal helix region in  S. typhimurium  (SEQ ID NO: 161-162), and  S. termitidis  (SEQ ID NO: 163-164). The region of the peptides in the native sequence is shown boxed in  FIG. 8  and the predicted helical targeting peptides are shown on the left panels. The peptides on the right panels in  FIGS. 9A and 9B  are helical wheel projections of the portion of the predicted peptide that map onto the consensus helical wheel prediction for all peptides 
         FIG. 10  shows the alignment and secondary structure prediction of B12-independent diol dehydratase showing interdomain peptide (Group 4). SEQ ID NOs: 323-334 correspond to ANHYDRO_00930 (SEQ ID NO: 323), PepasDRAFT_0461 (SEQ ID NO: 324), c4537 (SEQ ID NO: 325), AECO1_2293 (SEQ ID NO: 326), ecoli_01002098 (SEQ ID NO: 327), Rru_A0903 (SEQ ID NO: 328), Rpc_1163 (SEQ ID NO: 329), cbei_4061 (SEQ ID NO: 330), clobol_08236 (SEQ ID NO: 331), NT01CX_0498 (SEQ ID NO: 332), sputw3181_0427 (SEQ ID NO: 333) and SPUTCN32_0208 (SEQ ID NO: 334). 
         FIG. 11A  shows the alignment and secondary structure prediction of L-Fuculose phosphate aldolase C-terminal region (peptide) presumed to be encapsulated in BMCs of some Planctomycetes and selection of Firmicutes. SEQ ID NOs: 335-359 corresponds to CLOSTASPAR_02209 (SEQ ID NO: 335), BselDRAFT_1650 (SEQ ID NO: 336), ANACOL_01089 (SEQ ID NO: 337), CLOSTMETH_00022 (SEQ ID NO: 338), GCWU000342_00652 (SEQ ID NO: 339), ROSEINA2194_01705 (SEQ ID NO: 340), RUMOBE_00095 (SEQ ID NO: 341), Cphy_1177 (SEQ ID NO: 342), RUMGNA_01020 (SEQ ID NO: 343), IsopDRAFT_2610 (SEQ ID NO: 344), PM8797T_14741 (SEQ ID NO: 345), Plim_1747 (SEQ ID NO: 346), RB2568 (SEQ ID NO: 347), DSM3645_04920 (SEQ ID NO: 348) and Psta_3288 (SEQ ID NO: 349). 
         FIGS. 12-24  shows the helical wheel projection for various peptides from various organisms The helical wheel representative peptide on the right panel in  FIGS. 12-24  are the fragments of the larger peptide shown in the left, mapped onto the consensus peptide motif shown in  FIGS. 3B  and C according to the scheme described in  FIG. 3D . 
         FIG. 12  shows the EutE homologue from  C. phytofermentans  C-terminal peptide helical wheel representative peptides (SEQ ID NO: 165 and 166). 
         FIG. 13  shows the B12-independent propanediol dehydratase from  R. palustris  BisB18 Interdomain-linker peptide helical wheel representations (SEQ ID NO: 167 and 168). 
         FIG. 14  shows the B12-independent propanediol dehydratase from  C. phytofermentans  Interdomain-linker peptide helical wheel representations (SEQ ID NO: 169 and 170). 
         FIG. 15  shows the Fuculose phosphate aldolase from  C. phytofermentans  C-terminal peptide helical wheel representations (SEQ ID NO: 171 and 172). 
         FIG. 16  shows the Aldehyde dehydrogenase from  C. kluyveri  C-terminal peptide helical wheel representations (SEQ ID NO: 173 and 174). 
         FIG. 17  shows the Fuculose phosphate aldolase from  P. limnophilus  C-terminal peptide helical wheel representations (SEQ ID NO: 175 and 176). 
         FIG. 18  shows the Fuculose/rhamnose phosphate aldolase from  O. terrae  PB90-1 C-terminal peptide helical wheel representations (SEQ ID NO: 177 and 178). 
         FIG. 19  shows the Aldehyde dehydrogenase from  O. terrae  PB90-1 N-terminal peptide helical wheel representations (SEQ ID NO: 179 and 180). 
         FIG. 20  shows the Aldehyde dehydrogenase (Cphy_1416) from  C. phytofermentans  C-terminal peptide helical wheel representations (SEQ ID NO: 181 and 182). 
         FIG. 21  shows the Aldehyde dehydrogenase (Cphy_1428) from  C. phytofermentans  C-terminal peptide helical wheel representations (SEQ ID NO: 183 and 184). 
         FIG. 22  shows the Unknown glycyl radical enyzme (Cphy_1417) from  C. phytofermentans  N-terminal peptide helical wheel representations (SEQ ID NO: 185 and 186). 
         FIG. 23  shows the Aldehyde dehydrogenase from  M. smegmatis  C-terminal peptide helical wheel representations (SEQ ID NO: 187 and 188). 
         FIG. 24  shows the Aldehyde dehydrogenase from  H. ochraceum  N-terminal peptide helical wheel representations (SEQ ID NO: 189 and 190). 
     
    
    
     Table 4 is a compilation of Tables 1-3 plus additional notes and information. 
     DETAILED DESCRIPTION 
     Introduction 
     Bacterial microcompartments (BMCs) encapsulate functionally related proteins. The bacterial microcompartment shell is composed of multiple paralogs of proteins containing the BMC domain (Pfam 00936) and presumably a relatively small number of proteins containing the Pfam03319 domain. There is recognizable sequence homology among the &gt;2000 BMC domains in the sequence databases, suggesting that despite functional diversity and some differences in the morphology of a specific bacterial microcompartment type, there are conserved structural determinants for targeting and binding of the enzymes and auxiliary proteins that are encapsulated in BMCs. 
     BMC shell proteins and the components they encapsulate are typically found in gene clusters (putative operons). We have identified a common region of primary structure on a subset of the proteins presumed to be encapsulated in functionally diverse BMCs. The common region is ˜20 amino acids long and is located at either the N- or the C-terminus of encapsulated proteins, and in a few cases, in between domains of a single protein. This peptide is separated from the rest of the protein by a poorly conserved linker region that is rich in small amino acids. The peptide and linker are present on numerous proteins presumed to be targeted to the interiors of 11 of the 15 types of BMCs; for the remaining 4 types of BMCs, the identity of the encapsulated proteins remains unknown, however a subset of these proteins are expected to contain a similar peptide for targeting. 
     The similarity among peptides targeted to distinct bacterial types implies that the recognition site for the BMC targeting region is located on the BMC shell rather than on other encapsulated components of the BMCs, because the latter vary among BMC type. Sequence comparison indicates that the most strongly conserved positions among the more 2000 BMC shell proteins currently in the database are found at the edges of the shell proteins. 
     In vitro pull-down assays for interaction used the region found on the C-terminus of the CcmN gene as an isolated peptide (SEQ ID NO:1). The results indicated that the peptide interacted with shell proteins and the CA homolog, CcmM. Fusion of the peptide of SEQ ID NO:1 to YFP appears to result in targeting of the YFP to the carboxysome shell in the cyanobacterium  Synechococcus  PCC7942 (data not shown). 
     Thus the region of primary structure (the peptide) appears to be a universal targeting signal for BMCs (and is herein referred to as the “BMC targeting region”). 
     The secondary structure of the region is predicted to be a single alpha helix flanked on one or both sides by regions predicted to be coil. Most of the predicted alpha helices, which are observed in very different encapsulated proteins, are also predicted to be amphipathic; the helices tend to be characterized by a four (4) residue hydrophobic polar face (positions 10, 6, 9 and 13 in SEQ ID NO:45) opposite a polar face. The conservation of amino acid properties, but lack of absolute sequence identity at each position in the peptide among the targeting/localization regions likely arises from the variability in the amino acid sidechain properties of their cognate shell protein binding partners. However for a given peptide type (e.g. PduP or CcmN) the sequence conservation is strong. 
     Irrespective of its location in the polypeptide chain, the targeting peptide region is always adjacent to poorly conserved region of amino acids that is rich in proline, glycine, and alanine (the linker region). If the targeting region is located at the N-terminus of an encapsulated protein, it is followed by the linker region and subsequently the functional domain(s) of the protein (See  FIGS. 1, 2, 3, 5, 6, 8, 10 and 11 ). If the region is located on the C-terminus of an encapsulated protein, the functional domain of the protein, followed by the linker precedes it ( FIG. 1 ). If the region is in the middle of a protein encapsulated in a BMC it is flanked on both sides by linker regions ( FIG. 10 ). 
     All BMC targeting regions share general properties (predicted alpha helical conformation, adjacent to poorly conserved segment(s) of primary structure); for each type of encapsulated protein, for each functionally distinct BMC, we have also identified a consensus amino acid sequence for the targeting region specific to that BMC (Tables 1-3). 
     Thus, in one embodiment, a common motif found in a subset of proteins presumed to be encapsulated in functionally diverse bacterial microcompartments (BMCs). In another embodiment, targeting peptides which share general properties (predicted alpha helical conformation, flanked by poorly conserved segment(s) of primary structure); for each type of encapsulated protein, for various identified functionally distinct BMC proteins, an identified consensus amino acid sequence for the targeting peptide specific to each of the identified BMCs. 
     Definitions 
     The term “amphipathic alpha helix” or “amphipathic a helix” refers to a polypeptide sequence that can adopt a secondary structure that is helical with one surface, i.e., face, being polar and comprised primarily of hydrophilic amino acids (e.g., Asp, Glu, Lys, Arg, His, Gly, Ser, Thr, Cys, Tyr, Asn and Gln), and the other surface being a nonpolar face that comprises primarily hydrophobic amino acids (e.g., Leu, Ala, Val, Ile, Pro, Phe, Trp and Met) (see, e.g., Kaiser and Kezdy,  Ann. Rev. Biophys. Biophys. Chem.  16: 561 (1987), and  Science  223:249 (1984)). 
     The terms “polypeptide,” “peptide” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical mimetic of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers and non-naturally occurring amino acid polymer. Amino acid polymers may comprise entirely L-amino acids, entirely D-amino acids, or a mixture of L and D amino acids. The use of the term “peptide or peptidomimetic” in the current application merely emphasizes that peptides comprising naturally occurring amino acids as well as modified amino acids are contemplated 
     The terms “isolated,” “purified,” or “biologically pure” refer to material that is substantially or essentially free from components that normally accompany it as found in its native state. Purity and homogeneity are typically determined using analytical chemistry techniques such as polyacrylamide gel electrophoresis or high performance liquid chromatography. A protein that is the predominant species present in a preparation is substantially purified. The term “purified” denotes that a nucleic acid or protein gives rise to essentially one band in an electrophoretic gel. 
     The terms “identical” or percent “identity,” in the context of two or more polypeptide sequences (or two or more nucleic acids), refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same e.g., 60% identity, preferably 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity over a specified region (such as the first 15 out of the 18 amino acids of SEQ ID NO:1), when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. Such sequences are then said to be “substantially identical.” This definition also refers to the compliment of a test sequence. 
     For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters. For sequence comparison of nucleic acids and proteins, the BLAST and BLAST 2.0 algorithms and the default parameters discussed below are typically used. 
     The terms “nucleic acid” and “polynucleotide” are used interchangeably herein to refer to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form. The term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, which are synthetic, naturally occurring, and non-naturally occurring, which have similar binding properties as the reference nucleic acid, and which are metabolized in a manner similar to the reference nucleotides. Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl phosphonates, 2-O-methyl ribonucleotides, polypeptide-nucleic acids (PNAs). Unless otherwise indicated, a particular nucleic acid sequence also encompasses “conservatively modified variants” thereof (e.g., degenerate codon substitutions) and complementary sequences, as well as the sequence explicitly indicated. Specifically, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., Nucleic Acid Res. 19:5081 (1991); Ohtsuka et al.,  J. Biol. Chem.,  260:2605-2608 (1985); Rossolini et al.,  Mol. Cell. Probes,  8:91-98 (1994)). The term nucleic acid can be used interchangeably with gene, cDNA, mRNA, oligonucleotide, and polynucleotide. 
     An “expression vector” is a nucleic acid construct, generated recombinantly or synthetically, with a series of specified nucleic acid elements that permit transcription of a particular nucleic acid in a host cell. The expression vector can be part of a plasmid, virus, or nucleic acid fragment. Typically, the expression vector includes a nucleic acid to be transcribed operably linked to a promoter. 
     By “host cell” is meant a cell that contains an expression vector and supports the replication or expression of the expression vector. Host cells may be prokaryotic cells such as  E. coli,  or eukaryotic cells such as yeast, insect, amphibian, or mammalian cells such as CHO, HeLa and the like, e.g., cultured cells, explants, and cells in vivo. 
     A “label” or “detectable label” is a composition detectable by spectroscopic, photochemical, biochemical, immunochemical, or chemical means. For example, useful labels include radioisotopes (e.g.,  3 H,  35 S,  32 P  51 Cr, or  125 I), fluorescent dyes, electron-dense reagents, enzymes (e.g., alkaline phosphatase, horseradish peroxidase, or others commonly used in an ELISA), biotin, digoxigenin, or haptens and proteins for which antisera or monoclonal antibodies are available (e.g., the polypeptide such as SEQ ID NOS: 1 or 2 can be made detectable, e.g., by incorporating a radiolabel into the polypeptide, and used to detect antibodies specifically reactive with the polypeptide). 
     DESCRIPTIONS OF THE EMBODIMENTS 
     It will be readily understood by those of skill in the art that the foregoing polypeptides are not fully inclusive of the family of polypeptides of the present invention. In fact, using the teachings provided herein, other suitable polypeptides (e.g., conservative variants) can be routinely produced by, for example, conservative or semi-conservative substitutions (e.g., Asp (D) replaced by Glu (E)), extensions, deletions and the like. In addition, it is contemplated that using the motif described, other suitable polypeptides can be found and screened for desired targeting activities. 
     Regarding amphipathic α-helix peptides, hydrophobic amino acids are concentrated on one side of the helix, usually with polar or charged amino acids on the other. Different amino-acid sequences have different propensities for forming α-helical structure. Methionine, alanine, leucine, glutamate, and lysine all have especially high helix-forming propensities, whereas proline, glycine, tyrosine, and serine have relatively poor helix-forming propensities. Proline tends to break or kink helices because it cannot donate an amide hydrogen bond (having no amide hydrogen), and because its side chain interferes sterically. Its ring structure also restricts its backbone dihedral angle to the vicinity of −70°, which is less common in α-helices. One of skill understands that although proline may be present at certain positions in the sequences described herein, e.g., at certain positions in the sequence of SEQ ID NO:10 or 31, the presence of more than three prolines within the sequence would be expected to disrupt the helical structure. Accordingly, the polypeptides of the invention do not have more than three prolines, and commonly do not have more than two prolines, present at positions in the alpha-helix forming sequence. 
     In the presently described peptides and motif, hydrophobic amino acids are considered primarily to include amino acid residues, such as Ile (I), Leu (L), Val (V), Met (M), Phe (F), Tyr (Y), Ala (A), Trp (W). Polar uncharged amino acids are considered primarily to include amino acids such as Gln (Q), Asn (N), Thr (T), Ser (S), and Cys (C). Charged amino acids are considered primarily to include amino acids such as Asp (D), Glu (E), Arg (R), Lys (K), and His (H). When the polar uncharged residues out numbered the charged residues the amino acid property assigned was polar. Proline and glycine are considered neutral amino acids and are not assigned to a specific group. 
     Thus, in one embodiment, the present invention provides an isolated polypeptide comprising an amino acid sequence in the N-terminal or C-terminal region or inter-domain region of an enzyme in a BMC-associated metabolic pathway in a microorganism comprising the peptides of SEQ ID NOS: 1-192. Table 1 shows the BMC-associated pathway, and the protein and organisms where the peptide is used natively. Also shown is the GenBank Accession number of the protein and the confidence level of the functional prediction of the peptide. Also shown are four organisms and/or metabolic pathways where a conserved region for a peptide may be found using the description of the region as described herein. Each of the GenBank Accessions are hereby incorporated by reference. 
     
       
         
           
               
               
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                   
                 Confidence 
                   
                   
                   
                   
               
               
                 BMC-associated 
                 Level of 
                   
                 Peptide-containing 
                   
                 SEQ 
               
               
                 metabolic 
                 Functional 
                 Representative 
                 ORFs with Locus 
                 Accession 
                 ID 
               
               
                 pathway 
                 Prediction 
                 organism 
                 Tag 
                 Number 
                 NO: 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 1 
                 High (exp) 
                 
                   Synechococcus elongatus 
                 
                 CcmN Cterm 
                 YP_400441 
                 1 
               
               
                 Calvin cycle 
                   
                 PCC7942 
                 (Synpcc7942_1424) 
               
               
                 1. 
                 High (exp) 
                 
                   Synechococcus elongatus 
                 
                 CcaA 
                 YP_400464 
                 2 
               
               
                 Calvin cycle 
                   
                 PCC7942 
                 (Synpcc7942_1447) 
               
               
                 2. Ethanolamine 
                 High (exp) 
                 
                   Salmonella typhimurium 
                 
                 EutC Nterm 
                 NP_461392 
                 3 
               
               
                 utilization 
                   
                 LT2 (Proteobacteria) 
                 (STM2457) 
               
               
                   
                   
                 
                   Clostridium 
                 
               
               
                   
                   
                   phytofermentans  ISDg 
               
               
                   
                   
                 (Firmicutes) 
               
               
                 2. 
                 High (exp) 
                 
                   Salmonella typhimurium 
                 
                 EutE Nterm 
                 NP_461398 
                 4 
               
               
                 Ethanolamine 
                   
                 LT2 (Proteobacteria) 
                 (STM2463) 
               
               
                 utilization 
                   
                 
                   Clostridium 
                 
               
               
                   
                   
                   phytofermentans  ISDg 
               
               
                   
                   
                 (Firmicutes) 
               
               
                 2. 
                 High (exp) 
                 
                   Salmonella typhimurium 
                 
                 EutE (Cphy_2642) 
                 YP_001559742 
                 5 
               
               
                 Ethanolamine 
                   
                 LT2 (Proteobacteria) 
               
               
                 utilization 
                   
                 
                   Clostridium 
                 
               
               
                   
                   
                   phytofermentans  ISDg 
               
               
                   
                   
                 (Firmicutes) 
               
               
                 3. 
                 High (exp) 
                 
                   Salmonella typhimurium 
                 
                 PduD Nterm 
                 NP_460986 
                 6 
               
               
                 Propanediol 
                   
                 LT2 
                 (STM2041) 
               
               
                 utilization (B12 
               
               
                 dependent) 
               
               
                 3. Propanediol 
                 High (exp) 
                 
                   Salmonella typhimurium 
                 
                 PduE Nterm 
                 NP_460987 
                 7 
               
               
                 utilization (B12 
                   
                 LT2 
                 (STM2042) 
               
               
                 dependent) 
               
               
                 3. Propanediol 
                 High (exp) 
                 
                   Salmonella typhimurium 
                 
                 PduP Nterm 
                 NP_460996 
                 8 
               
               
                 utilization (B12 
                   
                 LT2 
                 (STM2051) 
               
               
                 dependent) 
               
               
                 4. 
                 High (pred) 
                 
                   Rhodopseudomonas 
                 
                 Putative B12- 
                 YP_531045 
                 9 
               
               
                 1,2-propanediol 
                   
                   palustris  BisB18 
                 independent 
               
               
                 utilization (B12 
                   
                   
                 propanediol 
               
               
                 independent) 
                   
                   
                 dehydratase 
               
               
                 (putative) 
                   
                   
                 (RPC_1163) 
               
               
                 4. 
                 High (pred) 
                 
                   Rhodopseudomonas 
                 
                 Aldehyde 
                 YP_531056 
                 10 
               
               
                 1,2-propanediol 
                   
                   palustris  BisB18 
                 dehydrogenase 
               
               
                 utilization (B12 
                   
                   
                 Nterm (RPC_1174) 
               
               
                 independent) 
               
               
                 (putative) 
               
               
                 5. Dissimilation of 
                 High (exp) 
                 
                   Clostridium 
                 
                 Putative B12- 
                 YP_001558291 
                 11 
               
               
                 fucose and 
                   
                   phytofermentans  ISDg 
                 independeent 
               
               
                 rhamnose to 
                   
                   
                 propanediol 
               
               
                 primary alcohols 
                   
                   
                 dehydratase 
               
               
                 (putative) 
                   
                   
                 (Cphy_1174) 
               
               
                 5. 
                 High (exp) 
                 
                   Clostridium 
                 
                 Fuculose-phosphate 
                 YP_001558294 
                 12 
               
               
                 Dissimilation of 
                   
                   phytofermentans  ISDg 
                 aldolase Cterm 
               
               
                 fucose and 
                   
                   
                 (Cphy_1177) 
               
               
                 rhamnose to 
               
               
                 primary alcohols 
               
               
                 (putative) 
               
               
                 5. 
                 High (exp) 
                 
                   Clostridium 
                 
                 Aldehyde 
                 YP_001558295 
                 13 
               
               
                 Dissimilation of 
                   
                   phytofermentans  ISDg 
                 dehydrogenase 
               
               
                 fucose and 
                   
                   
                 (Cphy_1178) Nterm 
               
               
                 rhamnose to 
               
               
                 primary alcohols 
               
               
                 (putative) 
               
               
                 6. 
                 High (exp) 
                   Clostridium kluyveri  DSM 
                 Aldehyde 
                 YP_001394464 
                 14 
               
               
                 Ethanol utilization 
                   
                 555 
                 dehydrogenases 
                 YP_001394466 
               
               
                   
                   
                   
                 Cterm (Ckl_1074) 
               
               
                   
                   
                   
                 (Ckl_1076) 
               
               
                 7. 
                 Medium 
                 
                   Planctomyces limnophilus 
                 
                 Aldolase Cterm 
                   
                 15 
               
               
                 Fuculose-1- 
                 (pred) 
                 DSM 3776 
                 (Plim_1747) 
               
               
                 phosphate 
               
               
                 metabolism 
               
               
                 (putative) 
               
               
                 7. 
                 Medium 
                 
                   Planctomyces limnophilus 
                 
                 Aldehyde 
                   
                 16 
               
               
                 Fuculose-1- 
                 (pred) 
                 DSM 3776 
                 dehydrogenase 
               
               
                 phosphate 
                   
                   
                 Nterm (Plim_1751) 
               
               
                 metabolism 
               
               
                 (putative) 
               
               
                 8. 
                 Medium 
                   Opitutus terrae  PB90-1 
                 Aldolase Cterm 
                 YP_001818183 
                 17 
               
               
                 Fuculose-1- 
                 (pred) 
                   
                 (Oter_1298) 
               
               
                 phosphate and 
               
               
                 rhamnulose-1- 
               
               
                 phosphate 
               
               
                 conversion to 
               
               
                 acetate or pyruvate 
               
               
                 (putative) 
               
               
                 8. 
                 Medium 
                   Opitutus terrae  PB90-1 
                 Aldehyde 
                 YP_001818180 
                 18 
               
               
                 Fuculose-1- 
                 (pred) 
                   
                 dehydrogenase 
               
               
                 phosphate and 
                   
                   
                 (Oter_1295) 
               
               
                 rhamnulose-1- 
               
               
                 phosphate 
               
               
                 conversion to 
               
               
                 acetate or pyruvate 
               
               
                 (putative) 
               
               
                 9. 
                 Medium 
                 
                   Clostridium 
                 
                 Aldehyde 
                 YP_001558530 
                 19 
               
               
                 Unknown glycyl 
                 (pred) 
                   phytofermentans  ISDg 
                 dehydrogenase I 
                 YP_001558542 
               
               
                 radical enzyme 
                   
                   
                 (Cphy_1416) Cterm 
               
               
                 (putative) 
                   
                   
                 Aldehyde 
               
               
                   
                   
                   
                 dehydrogenase II 
               
               
                   
                   
                   
                 (Cphy_1428) Cterm 
               
               
                 9. 
                 Medium 
                 
                   Clostridium 
                 
                 unknown glycyl 
                 YP_001558531 
                 20 
               
               
                 Unknown glycyl 
                 (pred) 
                   phytofermentans  ISDg 
                 radical enzyme Nterm 
               
               
                 radical enzyme 
                   
                   
                 (Cphy_1417) 
               
               
                 (putative) 
               
               
                 10. 
                 Med (pred) 
                 
                   Mycobacterium 
                 
                 Aldehyde 
                 YP_884691 
                 21 
               
               
                 Amino alcohol 
                 Urano et al., 
                   smegmatis  MC2 155 
                 dehydrogenase 
               
               
                 metabolism 
                 2011 
                   
                 Cterm 
               
               
                 (putative) 
                   
                   
                 (MSMEG_0276) 
               
               
                 11. 
                 Low (pred) 
                 
                   Haliangium ochraceum 
                 
                 Aldehyde 
                 ZP_03875711 
                 22 
               
               
                 Serine-threonine 
                   
                 SMP-2 
                 dehydrogenase 
               
               
                 metabolism 
                   
                   
                 Nterm 
               
               
                 (putative) 
                   
                   
                 (HochDRAFT_00990) 
               
               
                 12. 
                 Medium 
                 
                   Bacteroides capillosus 
                 
                 unknown 
                 unknown 
               
               
                 Glutamate-arginine 
                 (pred) 
                 ATCC 29799 
               
               
                 metabolism 
               
               
                 (putative) 
               
               
                 13. 
                 Low (pred) 
                 
                   Alkaliphilus 
                 
                 unknown 
                 Unknown 
               
               
                 Anaerobic purine 
                   
                   metalliredigens  QYMF 
               
               
                 metabolism 
               
               
                 (putative) 
               
               
                 14 
                 Low (pred) 
                 
                   Methylibium 
                 
                 unknown 
                 Unknown 
               
               
                 Unknown 
                   
                   petroleiphilum  PM1 
               
               
                 15 
                 Zero 
                 
                   Chloroherpeton 
                 
                 unknown 
                 Unknown 
               
               
                 Unknown 
                   
                   thalassium  ATCC 35110 
               
               
                   
               
            
           
         
       
     
     Table 2 shows the actual isolated peptide sequences from the localization region found in the proxy organisms. The BMC associated metabolic pathway is predicted based on experimental evidence and the annotation (using the Integrated Microbial Genomes database found at the Joint Genomes Institute website) of gene products clustered with BMC shell protein genes on the chromosome. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                   
                   
                   
                 Actual ORF peptide 
               
               
                   
                   
                   
                 sequence from 
               
               
                   
                   
                   
                 proxy organism 
               
               
                   
                   
                   
                 (BOLD = well 
               
               
                   
                   
                   
                 predicted helical 
               
               
                   
                   
                   
                 portion; italics =  
               
               
                 Peptide-containing ORFs 
                 Accession 
                 SEQ 
                 lower confidence in  
               
               
                 with Locus Tag 
                 Number 
                 ID NO: 
                 predicted helical portion) 
               
               
                   
               
             
            
               
                 CcmN Cterm 
                 YP_400441 
                  1 
                 
                   VYGKEQFLRMRQSMFPDR 
                 
               
               
                 (Synpcc7942_1424) 
                   
                   
                   
               
               
                   
               
               
                 CcaA (Synpcc7942_1447) 
                 YP_400464 
                  2 
                 
                   LAPEQQQRIYRGN 
                 
               
               
                   
               
               
                 EutC Nterm (STM2457) 
                 NP_461392 
                  3 
                 
                   MDQKQIEEIVRSVMAS 
                 
               
               
                   
               
               
                 EutE Nterm (STM2463) 
                 NP_461398 
                  4 
                 
                   MNQQDIEQVVKAVLLKM 
                 
               
               
                   
               
               
                 EutE (Cphy_2642) 
                 YP_001559742 
                  5 
                 
                   NTELVEEIVKRIMKQL 
                 
               
               
                   
               
               
                 PduD Nterm (STM2041) 
                 NP_460986 
                  6 
                 
                   MEINEKLLRQIIEDVLRDM 
                 
               
               
                   
               
               
                 PduE Nterm (STM2042) 
                 NP_460987 
                  7 
                 
                   MNTDAIESMVRDVLSRMNS 
                 
               
               
                   
               
               
                 PduP Nterm (STM2051) 
                 NP_460996 
                  8 
                 
                   MNTSELETLIRTILSE 
                 
               
               
                   
               
               
                 Putative B12 independent 
                 YP_531045 
                  9 
                 
                   AGTNYTEEQVFAAVKKVLNSSGSTDV 
                 
               
               
                 propanediol dehydratase 
                   
                   
                   
               
               
                 inter-domain (RPC_1163) 
                   
                   
                   
               
               
                   
               
               
                 Aldehyde dehydrogenase 
                 YP_531056 
                 10 
                 
                   MVAKAIRDHAGTAQPSGNA 
                 
               
               
                 Nterm (RPC_1174) 
                   
                   
                   
               
               
                   
               
               
                 Putative B12-independeent 
                 YP_001558291 
                 11 
                 
                   IDIILAQQITVQIVKELKERG 
                 
               
               
                 propanediol dehydratase 
                   
                   
                   
               
               
                 inter-domain (Cphy_1174) 
                   
                   
                   
               
               
                   
               
               
                 Fuculose-phosphate 
                 YP_001558294 
                 12 
                 
                   DNADLVASITRKVMEQLG 
                 
               
               
                 aldolase Cterm 
                   
                   
                   
               
               
                 (Cphy_1177) 
                   
                   
                   
               
               
                   
               
               
                 Aldehyde dehydrogenase 
                 YP_001558295 
                 13 
                 
                   VNEQLVQDIIKNVVASMQLT 
                 
               
               
                 (Cphy_1178) Nterm 
                   
                   
                   
               
               
                   
               
               
                 Aldehyde dehydrogenases 
                 YP_001394464 
                 14 
                 
                   EPEDNEDVQAIVKAIMAKLNL 
                 
               
               
                 Cterm(Ckl_1074) 
                 YP_001394466 
                   
                   
               
               
                 (Ckl_1076) 
                   
                   
                   
               
               
                   
               
               
                 Aldolase Cterm 
                   
                 15 
                 
                   DTEMLVKMITEQVMAALKK 
                 
               
               
                 (Plim_1747) 
                   
                   
                   
               
               
                   
               
               
                 Aldehyde dehydrogenase 
                   
                 16 
                 
                   MQATEQAIRQVVQEVLAQLN 
                 
               
               
                 Nterm (Plim_1751) 
                   
                   
                   
               
               
                   
               
               
                 Aldolase Cterm 
                 YP_001818183 
                 17 
                 
                   EVEALVQRLTEEILRQLQ 
                 
               
               
                 (Oter_1298) 
                   
                   
                   
               
               
                   
               
               
                 Aldehyde dehydrogenase 
                 YP_001818180 
                 18 
                 
                   IDETLVRSVVEEVVRAF 
                 
               
               
                 (Oter_1295) 
                   
                   
                   
               
               
                   
               
               
                 Aldehyde dehydrogenase I 
                 YP_001558530 
                 19 
                 
                   EDARDLLKQILQALS 
                 
               
               
                 (Cphy_1416) Cterm 
                   
                   
                   
               
               
                 Aldehyde dehydrogenase II 
                 YP_001558542 
                   
                   
               
               
                 (Cphy_1428) Cterm 
                   
                   
                   
               
               
                   
               
               
                 Unknown glycyl radical 
                 YP_001558531 
                 20 
                 
                   MDIREFSNKFVEATKNM 
                 
               
               
                 enzyme Nterm (Cphy_1417) 
                   
                   
                   
               
               
                   
               
               
                 Aldehyde dehydrogenase 
                 YP_884691 
                 21 
                 
                   LDALRAELRALVVEELAQLIKR 
                 
               
               
                 Cterm (MSMEG_0276) 
                   
                   
                   
               
               
                   
               
               
                 Aldehyde dehydrogenase 
                 ZP_03875711 
                 22 
                 
                   MALREDRIAEIVERVLARL 
                 
               
               
                 Nterm (HochDRAFT_00990) 
               
               
                   
               
            
           
         
       
     
     In another embodiment, consensus peptides SEQ ID NOS: 23-45 are provided for specific BMC-associated pathway enzymes and proteins as shown in Table 3. The residues in parentheses and separated by slashes in the consensus peptides represent that the amino acid at that residue position in the peptide can be chosen from any of the amino acids shown in the parenthesis. 
     
       
         
           
               
               
               
             
               
                 TABLE 3 
               
               
                   
               
               
                   
                   
                 Metabolic group 
               
               
                 BMC-associated 
                 SEQ 
                 peptide consensus 
               
               
                 metabolic pathway 
                 ID NO: 
                 (from alignment) 
               
               
                   
               
             
            
               
                 Calvin cycle 
                 23 
                 (V/I)(V/Y)G(Q/K) 
               
               
                   
                   
                 (V/A/G/E)(Y/S/Q) 
               
               
                   
                   
                 (I/V/L/F)(N/Q/S/L) 
               
               
                   
                   
                 (K/Q/R)(M/L) 
               
               
                   
                   
                 (L/M/R)(V/L/C/Q) 
               
               
                   
                   
                 (T/S)(L/M)FP 
               
               
                   
                   
                 (H/D/E)(R/N/Q) 
               
               
                   
               
               
                 Calvin cycle 
                 24 
                 (L/F)(S/P/A)(P/V) 
               
               
                   
                   
                 (E/Q)Q(A/S/Q/W) 
               
               
                   
                   
                 (Q/E/R)RIY(R/Q)G 
               
               
                   
                   
                 (S/N) 
               
               
                   
               
               
                 Ethanolamine 
                 25 
                 M(D/N)(E/Q)(K/Q) 
               
               
                 utilization 
                   
                 (Q/E)(L/I)(K/R/E) 
               
               
                   
                   
                 (E/D)(I/M)(V/I) 
               
               
                   
                   
                 (R/E)(S/Q)(V/I) 
               
               
                   
                   
                 (L/M)A(E/Q/S) 
               
               
                   
               
               
                 Ethanolamine 
                 26 
                 MNQQDIEQVVKAVLLKM 
               
               
                 utilization 
                   
                   
               
               
                   
               
               
                 Ethanolamine 
                 27 
                 (A/K/S)(E/D)(A/E) 
               
               
                 utilization 
                   
                 L(I/V)(E/D/N) 
               
               
                   
                   
                 (L/E/S)(I/L)(V/I) 
               
               
                   
                   
                 (R/K/E/Q)(K/R)VL 
               
               
                   
                   
                 (E/A)(E/K)L 
               
               
                   
               
               
                 Propanediol 
                 28 
                 MEI(N/D/T)E(K/E) 
               
               
                 utilization 
                   
                 (L/V)(L/V)(R/E)Q 
               
               
                 (B12 dependent) 
                   
                 (I/V)(I/V)(E/K/A) 
               
               
                   
                   
                 (D/E)VL(K/S/R/A) 
               
               
                   
                   
                 (E/D)(M/L) 
               
               
                   
               
               
                 Propanediol 
                 29 
                 (M/I)(N/D)(T/E) 
               
               
                 utilization 
                   
                 (D/K)(A/L)(I/L)E 
               
               
                 (B12 dependent) 
                   
                 (S/E)(M/I)V(R/K) 
               
               
                   
                   
                 (D/E/Q)VL(S,N) 
               
               
                   
                   
                 (M/L)(N/E/G)S 
               
               
                   
               
               
                 Propanediol 
                 30 
                 M(N/D/E)(T/S/E) 
               
               
                 utilization 
                   
                 (S/L)E(L/V)E 
               
               
                 (B12 dependent) 
                   
                 (T/Q/K/D)(L/I) 
               
               
                   
                   
                 (I/V)(R/K)(T/N/K) 
               
               
                   
                   
                 (I/V)(L/I) 
               
               
                   
                   
                 (S/L/R/N)E 
               
               
                   
               
               
                 1,2-propanediol 
                 31 
                 (A/P)(K/G)(S/Q) 
               
               
                 utilization (B12 
                   
                 (S/D)(L/A)(T/N)E 
               
               
                 independent) 
                   
                 (E/Q)(D/Q)(I/V)Y 
               
               
                 (putative) 
                   
                 (D/E)AVK(K/R) 
               
               
                   
                   
                 (V/I)(L/I)(E/G) 
               
               
                   
                   
                 (Q/E/S)(H/S)G 
               
               
                   
                   
                 (A/S)LD(P/V) 
               
               
                   
               
               
                 1,2-propanediol 
                 32 
                 MN(D/T)(I/T)(E/Q) 
               
               
                 utilization (B12 
                   
                 (I/L)(A/E)(Q/N) 
               
               
                 independent) 
                   
                 (A/M)(V/I)(S/R/A) 
               
               
                 (putative) 
                   
                 (T/K/N)IL 
               
               
                   
                   
                 (S/A/E/R)(D/K) 
               
               
                   
                   
                 (N/F/Y)(T/L/G)K 
               
               
                   
               
               
                 Dissimilation of 
                 33 
                 LD(A/E)ES(A/V) 
               
               
                 fucose and rhamnose 
                   
                 (A/G)D(M/I)(T/A) 
               
               
                 to primary alcohols 
                   
                 E(M/Q)I(A/L)K 
               
               
                 (putative) 
                   
                 (E/G)(L/M)(K/Q) 
               
               
                   
                   
                 (E/D)AG 
               
               
                   
               
               
                 Dissimilation of 
                 34 
                 (D/P)(D/N)(A/E) 
               
               
                 fucose and rhamnose 
                   
                 (D/E/A)L(V/I)A 
               
               
                 to primary alcohols 
                   
                 (E/A/S)IT(K/R) 
               
               
                 (putative) 
                   
                 (K/R/Q)V(M/L) 
               
               
                   
                   
                 (A/E)QL(G/K) 
               
               
                   
               
               
                 Dissimilation of 
                 35 
                 VNEQ(L/M)VQDIV 
               
               
                 fucose and rhamnose 
                   
                 (Q/R/K)EVVA(K/R) 
               
               
                 to primary alcohols 
                   
                 MQI(S/T) 
               
               
                 (putative) 
                   
                   
               
               
                   
               
               
                 Ethanol utilization 
                 36 
                 EPEDNEDVQAIVKAIM 
               
               
                   
                   
                 AKLNL Aldehyde 
               
               
                   
                   
                 dehydrogenase  
               
               
                   
                   
                 Cterm- unique as 
               
               
                   
                   
                 a group but 
               
               
                   
                   
                 similar to other 
               
               
                   
                   
                 Cterm Aldehyde 
               
               
                   
                   
                 dehydrogenase 
               
               
                   
                   
                 tags 
               
               
                   
               
               
                 Fuculose-1- 
                 37 
                 DQE(A/Q)LV(K/Q) 
               
               
                 phosphate 
                   
                 (A/L)IT(D/E) 
               
               
                 metabolism (putative) 
                   
                 (Q/R/E)VMA(A/E) 
               
               
                   
                   
                 L(K/S)K 
               
               
                   
               
               
                 Fuculose-1- 
                 38 
                 MQ(I/A)(D/T)EE 
               
               
                 phosphate 
                   
                 (L/A)IRSVV(A/Q) 
               
               
                 metabolism (putative) 
                   
                 (Q/E)VL(A/S) 
               
               
                   
                   
                 (E/Q)(V/L)(G/N) 
               
               
                   
               
               
                 Fuculose-1- 
                 39 
                 EVEALVQRLTEEILRQ 
               
               
                 phosphate and 
                   
                 LQ Aldolase 
               
               
                 rhamnulose-1- 
                   
                 Cterm- unique as 
               
               
                 phosphate conversion 
                   
                 a group but 
               
               
                 to acetate or  
                   
                 similar to other 
               
               
                 pyruvate (putative) 
                   
                 Cterm aldolase 
               
               
                   
                   
                 tags 
               
               
                   
               
               
                 Fuculose-1- 
                 40 
                 IDETLVRSWEEWRAF 
               
               
                 phosphate and 
                   
                 Aldehyde 
               
               
                 rhamnulose-1- 
                   
                 dehydrogenase 
               
               
                 phosphate conversion 
                   
                 Nterm- unique as 
               
               
                 to acetate or  
                   
                 a group but 
               
               
                 pyruvate (putative) 
                   
                 similar to other 
               
               
                   
                   
                 Nterm Aldehyde 
               
               
                   
                   
                 dehydrogenase 
               
               
                   
                   
                 tags 
               
               
                   
               
               
                 Unknown glycyl 
                 41 
                 (E/Q/D)(N/E/D) 
               
               
                 radical enzyme 
                   
                 (V/I/L)(E/Q/A) 
               
               
                 (putative) 
                   
                 (R/Q/D)(I/L/V) 
               
               
                   
                   
                 (I/L/V)(K/R/N) 
               
               
                   
                   
                 (E/Q/K)(V/I/L) 
               
               
                   
                   
                 (L/I/V)(E/Q/G) 
               
               
                   
                   
                 (Q/R/A)(L/M) 
               
               
                   
                   
                 (K/G/S) 
               
               
                   
               
               
                 Unknown glycyl 
                 42 
                 M(A/D)(K/I/N/L) 
               
               
                 radical enzyme 
                   
                 (R/Y/)(E/N/S/) 
               
               
                 (putative) 
                   
                 (L/F)(T/S)(P/N) 
               
               
                   
                   
                 (R/K)(V/L/F)(K/A) 
               
               
                   
                   
                 (E/V/M)(L/A)(A/T) 
               
               
                   
                   
                 (E/K)(R/N)(L/M) 
               
               
                   
               
               
                 Arginine or 
                 43 
                 I(E/D/G)ALR 
               
               
                 serine/threonine 
                   
                 (A/E/D)ELR(A/R)L 
               
               
                 metabolism (putative) 
                   
                 (V/I)(V/A)EEL 
               
               
                   
                   
                 (A/R)(Q/E)L 
               
               
                   
                   
                 (I/N/G)(K/R)(R/Q) 
               
               
                   
               
               
                 Serine-threonine 
                 44 
                 MALREDRIAEIVERVLA 
               
               
                 metabolism (putative) 
                   
                 RL unique as  
               
               
                   
                   
                 group but similar 
               
               
                   
                   
                 to other Nterm 
               
               
                   
                   
                 Aldehyde 
               
               
                   
                   
                 dehydrogenase 
               
               
                   
                   
                 tags 
               
               
                   
               
            
           
         
       
     
     Table 4 is a compilation of Tables 1-3 plus additional notes and information. 
     
       
         
           
               
               
               
               
               
               
               
             
               
                 TABLE 4 
               
               
                   
               
             
            
               
                   
                   
                   
                   
                   
                 Actual ORF peptide 
                   
               
               
                   
                   
                   
                   
                   
                 sequence from 
                   
               
               
                   
                   
                   
                   
                   
                 proxy organism 
                   
               
               
                   
                   
                   
                   
                 Peptide- 
                 (BOLD = well 
                   
               
               
                   
                   
                   
                   
                 containing 
                 predicted 
                   
               
               
                   
                 BMC- 
                 Confidence 
                   
                 ORFs with Locus 
                 helical portion.  
                   
               
               
                   
                 associated 
                 Level of 
                   
                 Tag and 
                 RED = not 
                 Metabolic group 
               
               
                 Group 
                 metabolic 
                 Functional 
                 Representative 
                 Accession 
                 well predicted 
                 peptide consensus 
               
               
                 # 
                 pathway 
                 Prediction 
                 organism 
                 Number 
                 helical portion) 
                 (from alignment) 
               
               
                   
               
               
                  1 
                 Calvin cycle 
                 High 
                 
                   Synechococcus 
                 
                 CcmN 
                 CcmN Cterm- 
                 CcmN Cterm- 
               
               
                   
                   
                 (exp) 
                 
                   elongatus  
                 
                 (Synpcc7942_1424) 
                 
                   VYGKEQFLRMRQSMFPDR 
                 
                 (V/I)(V/Y)G(Q/K)(V/A/G/E)(Y/S/Q)(I/V/L/F) 
               
               
                   
                   
                   
                 PCC7942 
                 YP_400441 
                 CcaA Cterm- 
                 (N/Q/S/L)(K/Q/R)(M/L)(L/M/R)(V/L/C/Q) 
               
               
                   
                   
                   
                   
                 CcaA 
                 
                   LAPEQQQRIYRGN 
                 
                 (T/S)(L/M)FP(H/D/E)(R/N/Q) 
               
               
                   
                   
                   
                   
                 (Synpcc7942_1447) 
                   
                 CcaA Cterm- 
               
               
                   
                   
                   
                   
                 YP_400464 
                   
                 (L/F)(S/P/A)(P/V)(E/Q)Q(A/S/Q/W)(Q/E/R) 
               
               
                   
                   
                   
                   
                   
                   
                 RIY(R/Q)G(S/N) 
               
               
                   
               
               
                  2 
                 Ethanolamine 
                 High 
                 
                   Salmonella 
                 
                 EutC (STM2457) 
                 EutC Nterm- 
                 EutC Nterm (firmicute/proteobacteria) 
               
               
                   
                 utilization 
                 (exp) 
                 
                   typhimurium 
                 
                 NP_461392 
                 
                   MDQKQIEEIVRSVMAS 
                 
                 M(D/N)(E/Q)(K/Q)(Q/E)(L/I)(K/R/E)(E/D) 
               
               
                   
                   
                   
                 LT2 
                 EutE (STM2463) 
                 EutE Nterm 
                 (I/M)(V/I)(R/E)(S/Q)(V/I)(L/M)A(E/Q/S) 
               
               
                   
                   
                   
                 (Proteobacteria) 
                 NP_461398 
                 (Proteobacteria)- 
                 EutE Nterm (Proteobacteria)- 
               
               
                   
                   
                   
                 Clostridium 
                 EutE (Cphy_1542) 
                 MNQQDIEQVVKAVLLKM 
                 MNQQDIEQVVKAVLLKM 
               
               
                   
                   
                   
                 phytofermentans 
                 YP_001559742 
                 EutE Cterm 
                 EutE Cterm (Firmicutes)- 
               
               
                   
                   
                   
                 ISDg 
                   
                 (Firmicutes)- 
                 (A/K/S)(E/D)(A/E)L(I/V)(E/D/N)(L/E/S) 
               
               
                   
                   
                   
                 (Firmicutes) 
                   
                 
                   NTELVEEIVKRIMKQL 
                 
                 (I/L)(V/I)(R/K/E/Q)(K/R)VL(E/A)(E/K)L 
               
               
                   
               
               
                  3 
                 Propanediol 
                 High 
                 
                   Salmonella 
                 
                 PduD (STM2041) 
                 PduD Nterm- 
                 PduD Nterm- 
               
               
                   
                 utilization 
                 (exp) 
                   typhimurium _T2 
                 NP_460986 
                 
                   MEINEKLLRQIIEDVLRDM 
                 
                 MEI(N/D/T)E(K/E)(L/V)(L/V)(R/E)Q(I/V) 
               
               
                   
                 (B12 
                   
                   
                 PduE (STM2042) 
                 PduE Nterm- 
                 (I/V)(E/K/A)(D/E)VL(K/S/R/A)(E/D)(M/L) 
               
               
                   
                 dependent) 
                   
                   
                 NP_460987 
                 
                   MNTDAIESMVRDVLSRMNS 
                 
                 PduE Nterm- 
               
               
                   
                   
                   
                   
                 PduP (STM2051) 
                 PduP Nterm- 
                 (M/I)(N/D)(T/E)(D/K)(A/L)(I/L)E(S/E)(M/I) 
               
               
                   
                   
                   
                   
                 NP_460996 
                 MNTSELETLIRTILSE 
                 V(R/K)(D/E/Q)VL(S/N)(M/L)(N/E/G)S 
               
               
                   
                   
                   
                   
                   
                   
                 PduP Nterm- 
               
               
                   
                   
                   
                   
                   
                   
                 M(N/D/E)(T/S/E)(S/L)E(L/V)E(T/Q/K/D)(L/I) 
               
               
                   
                   
                   
                   
                   
                   
                 (I/V)(R/K)(T/N/K)(I/V)(L/I)(S/L/R/N)E 
               
               
                   
               
               
                  4 
                 1,2- 
                 High 
                 
                   Rhodopseudomonas 
                 
                 Putative 
                 Pdu Interdomain  
                 Pdu (B12-independent)- 
               
               
                   
                 propanediol 
                 (pred) 
                   palustris  BisB18 
                 B12-independent 
                 linker- 
                 (A/P)(K/G)(S/Q)(S/D)(L/A)(T/N)E(E/Q)(D/Q) 
               
               
                   
                 utilization 
                   
                   
                 propanediol 
                 
                   AGTNYTEEQVFAAVKKVLNSSGSTDV 
                 
                 (I/V)Y(D/E)AVK(K/R)(V/I)(L/I)(E/G)(Q/E/S) 
               
               
                   
                 (B12 
                   
                   
                 dehydratase 
                 Aldehyde dehydrogenase  
                 (H/S)G(A/S)LD(P/V) 
               
               
                   
                 independent) 
                   
                   
                 (RPC_1163) 
                 Nterm- 
                 Aldehyde dehydrogenase Nterm- 
               
               
                   
                 (putative) 
                   
                   
                 YP_531045 
                 MVAKAIRDHAGTAQPSGNA 
                 MN(D/T)(I/T)(E/Q)(I/L)(A/E)(Q/N)(A/M) 
               
               
                   
                   
                   
                   
                 Aldehyde 
                   
                 (V/I)(S/R/A)(T/K/N)IL(S/A/E/R)(D/K) 
               
               
                   
                   
                   
                   
                 dehydrogenase 
                   
                 (N/F/Y)(T/L/G)K 
               
               
                   
                   
                   
                   
                 (RPC_1174) 
                   
                   
               
               
                   
                   
                   
                   
                 YP_531056 
                   
                   
               
               
                   
               
               
                  5 
                 Dissimitation 
                 High 
                 
                   Clostridium 
                 
                 Putative 
                 Cphy_1174 interdomain  
                 Pdu (B12-independent)- 
               
               
                   
                 of fucose and 
                 (exp) 
                 
                   phytofermentans 
                 
                 B12-independeent 
                 linker- 
                 EVGE(D/K)EIAA9I/V)LXTVLE(A/M)(E/K)LP 
               
               
                   
                 rhamnose to 
                   
                 ISDg 
                 propanediol 
                 
                   EKEIEQILKTVLEAKKENTE 
                 
                 Fuculose-phosphate aldolase Cterm- 
               
               
                   
                 primary 
                   
                   
                 dehydratase 
                 Cphy_1177 Cterm- 
                 (D/P)(D/N)(A/E)(D/E/A)L(V/I)A(E/A/S)IT 
               
               
                   
                 alcohols 
                   
                   
                 (Cphy_1174) 
                 
                   DNADLVASITRKVMEQLG 
                 
                 (K/R)(K/R/Q)V(M/L)(A/E)QL(G/K) 
               
               
                   
                 (putative 
                   
                   
                 YP_001558291 
                 Cphy_1178 Nterm- 
                 Aldehyde dehydrogenase N-term- 
               
               
                   
                   
                   
                   
                 Fuculose- 
                 VNEQLVQDIIKNVVASMQLT 
                 VNEQ(L/M)VQDIV(Q/R/K)EVVA(K/R)MQ(S/T) 
               
               
                   
                   
                   
                   
                 phosphate 
                   
                   
               
               
                   
                   
                   
                   
                 aldolase 
                   
                   
               
               
                   
                   
                   
                   
                 (cphy_1177) 
                   
                   
               
               
                   
                   
                   
                   
                 YP_001558294 
                   
                   
               
               
                   
                   
                   
                   
                 Aldehyde 
                   
                   
               
               
                   
                   
                   
                   
                 dehydrogenase 
                   
                   
               
               
                   
                   
                   
                   
                 (Cphy_1178) 
                   
                   
               
               
                   
                   
                   
                   
                 YP_001558295 
                   
                   
               
               
                   
               
               
                  6 
                 Ethanol 
                 High 
                 
                   Clostridium 
                 
                 Aldehyde 
                 Aldehyde dehydrogenase 
                 Aldehyde dehydrogenase Cterm- 
               
               
                   
                 utilization 
                 (exp) 
                 
                   kluyveri 
                 
                 dehydrogenases 
                 Cterm- 
                 unique as a group but similar to other 
               
               
                   
                   
                   
                 DSM 555 
                 (Ckl_1074) 
                 
                   EPEDNEDVQAIVKAIMAKLNL 
                 
                 Cterm Aldehyde dehydrogenase tags 
               
               
                   
                   
                   
                   
                 YP_001394464 
                   
                   
               
               
                   
                   
                   
                   
                 (Ckl_1076) 
                   
                   
               
               
                   
                   
                   
                   
                 YP_001394466 
                   
                   
               
               
                   
               
               
                  7 
                 Fuculose-1- 
                 Medium 
                 
                   Planctomyces 
                 
                 Aldolase 
                 Plim_1747 Cterm- 
                 Aldolase Cterm- 
               
               
                   
                 phosphate 
                 (pred) 
                 
                   limnophilus 
                 
                 (Plim_1747) 
                 
                   DTEMLVKMITEQVMAALKK 
                 
                 DQE(A/Q)LV(K/Q)(A/L)IT(D/E)(Q/R/E)V 
               
               
                   
                 metabolism 
                   
                 DSM 3776 
                 Aldehyde 
                 Plim_1751 Nterm- 
                 MA(A/E)L(K/S)K 
               
               
                   
                 (putative) 
                   
                   
                 dehydrogenase 
                 MQATEQAIRQVVQEVLAQLN 
                 Aldehyde dehydrogenase Nterm- 
               
               
                   
                   
                   
                   
                 (Plim_1751) 
                   
                 MQ(I/A)(D/T)EE(L/A)IRSVV(A/Q)(Q/E)V 
               
               
                   
                   
                   
                   
                   
                   
                 L(A/S)(E/Q)(V/L)(G/N) 
               
               
                   
               
               
                  8 
                 Fuculose-1- 
                 Medium 
                 
                   Opitutus terrae 
                 
                 Aldolase 
                 Oter_1298 Cterm- 
                 Aldolase Cterm- unique as a group but 
               
               
                   
                 phosphate and 
                 (pred) 
                 PB90-1 
                 (Oter_1298) 
                 
                   EVEALVQRLTEEILRQLQ 
                 
                 similar to other Cterm aldolase tags 
               
               
                   
                 rhamnulose-1- 
                   
                   
                 YP_001818183 
                 Oter_1295 Nterm- 
                 Aldehyde dehydrogenase Nterm- 
               
               
                   
                 phosphate 
                   
                   
                 Aldehyde 
                 
                   IDETLVRSVVEEVVRAF 
                 
                 unique as a group but similar to other 
               
               
                   
                 conversion to 
                   
                   
                 dehydrogenase 
                   
                 Nterm Aldehyde dehydrogenase tags 
               
               
                   
                 acetate or 
                   
                   
                 (Oter_1295) 
                   
                   
               
               
                   
                 pyruvate 
                   
                   
                 YP_001818180 
                   
                   
               
               
                   
                 (putative) 
                   
                   
                   
                   
                   
               
               
                  9 
                 Unknown 
                 Medium 
                 
                   Clostridium 
                 
                 Aldehyde 
                 Cphy_1416 Cterm- 
                 Aldehyde dehydrogenase Cterm- 
               
               
                   
                 glycyl 
                 (pred) 
                 
                   Phytofermentans 
                 
                 dehydrogenase I 
                 
                   EDARDLLKQILQALS 
                 
                 (E/Q/D)(N/E/D)(V/I/L)(E/Q/A)(R/Q/D) 
               
               
                   
                 radical 
                   
                 ISDg 
                 (Cphy_1416) 
                 Cphy_1417 Nterm- 
                 (I/L/V)(K/R/N)(E/Q/K)(V/I/L)(L/I/V) 
               
               
                   
                 enzyme 
                   
                   
                 YP_001558530 
                 
                   MDIREFSNKFVEATKNM 
                 
                 (E/Q/G)(Q/R/A)(L/M)(K/G/S) 
               
               
                   
                 (putative) 
                   
                   
                 Aldehyde 
                   
                 Unknown glycyl radical enzyme Nterm- 
               
               
                   
                   
                   
                   
                 dehydrogenase II 
                   
                 M(A/D)(K/I/N/L)(R/Y/)(E/N/S/)(L/F)(T/S) 
               
               
                   
                   
                   
                   
                 (Cphy_1428) 
                   
                 (P/N)(R/K)(V/L/F)(K/A)(E/V/M)(L/A)(A/T) 
               
               
                   
                   
                   
                   
                 YP_001558542 
                   
                 (E/K)(R/N)(L/M) 
               
               
                   
                   
                   
                   
                 unknown glycyl 
                   
                   
               
               
                   
                   
                   
                   
                 radical enzyme 
                   
                   
               
               
                   
                   
                   
                   
                 (Cphy_1417) 
                   
                   
               
               
                   
                   
                   
                   
                 YP_001558531 
                   
                   
               
               
                   
               
               
                 10 
                 Arginine or 
                 Low 
                 
                   Mycobacterium 
                 
                 Aldehyde 
                 MSMEG_0276 Cterm- 
                 Aldehyde dehydrogenase Cterm- 
               
               
                   
                 serine/ 
                 (pred) 
                 
                   smegmatis 
                 
                 dehydrogenase 
                 
                   LDALRAELRALVVEELAQLIKR 
                 
                 I(E/D/G)ALR(A/E/D)ELR(A/R)L(V/I)(V/A) 
               
               
                   
                 threonine 
                   
                 MC2 155 
                 (MSMEG_0276) 
                   
                 EEL(A/R)(Q/E)L(I/N/G)(K/R)(R/Q) 
               
               
                   
                 metabolism 
                   
                   
                 YP_884691 
                   
                   
               
               
                   
                 (putative) 
                   
                   
                   
                   
                   
               
               
                   
               
               
                 11 
                 Serine- 
                 Low 
                 
                   Haliangium 
                 
                 Aldehyde 
                 HochDRAFT_00990 Nterm- 
                 Aldehyde dehydrogenase Nterm- 
               
               
                   
                 threonine 
                 (pred) 
                 
                   ochraceum 
                 
                 dehydrogenase 
                 
                   MALREDRIAEIVERVLARL 
                 
                 unique as a group but similar to other 
               
               
                   
                 metabolism 
                   
                 SMP-2 
                 (HochDRAFT_00990) 
                   
                 Nterm Aldehyde dehydrogenase tags 
               
               
                   
                 (putative) 
                   
                   
                 ZP_03875711 
                   
                   
               
               
                   
               
               
                 12 
                 Glutamate- 
                 Medium 
                 
                   Bacteroides 
                 
                 unknown 
                 unknown 
                 unknown 
               
               
                   
                 arginine 
                 (pred) 
                 
                   capillosus 
                 
                   
                   
                   
               
               
                   
                 metabolism 
                   
                 ATCC 29799 
                   
                   
                   
               
               
                   
                 (putative) 
                   
                   
                   
                   
                   
               
               
                   
               
               
                 13 
                 Anaerobic 
                 Low 
                 
                   Alkaliphilus 
                 
                 unknown 
                 unknown 
                 unknown 
               
               
                   
                 purine 
                 (pred) 
                 
                   metalliredigens 
                 
                   
                   
                   
               
               
                   
                 metabolism 
                   
                 QYMF 
                   
                   
                   
               
               
                   
                 (putative) 
                   
                   
                   
                   
                   
               
               
                   
               
               
                 14 
                 Unknown 
                 Low 
                 
                   Methylibium 
                 
                 unknown 
                 unknown 
                 unknown 
               
               
                   
                   
                 (pred) 
                 
                   petroleiphilum 
                 
                   
                   
                   
               
               
                   
                   
                   
                 PM1 
                   
                   
                   
               
               
                   
               
               
                 15 
                 Unknown 
                 Zero 
                 
                   Chloroherpeton 
                 
                 unknown 
                 unknown 
                 unknown 
               
               
                   
                   
                   
                 
                   thalassium 
                 
                   
                   
                   
               
               
                   
                   
                   
                 ATCC 35110 
               
               
                   
               
               
                   
                 Potentially 
                   
                   
                   
                   
                   
               
               
                 Group 
                 encapsulated 
                 GOID 
                 Organism 
                   
                 Reason for 
                   
               
               
                 # 
                 reactions 
                 range 
                 phenotypes 
                 Enzymes 
                 encapsulation 
                 Additional Notes 
               
               
                   
               
               
                  1 
                 Bicarbonate → 
                 637799853- 
                 Aerobe 
                 Carbonic 
                 RuBisCO 
                   
               
               
                   
                 carbon 
                 637799857 
                   
                 anhydrase, 
                 inefficiency 
                   
               
               
                   
                 dioxide → 
                   
                   
                 RuBisCO 
                 RuBisCO oxygen 
                   
               
               
                   
                 glycerate3- 
                   
                   
                   
                 sensativity 
                   
               
               
                   
                 phosphate 
                   
                   
                   
                 product 
                   
               
               
                   
                   
                   
                   
                   
                 toxicity 
                   
               
               
                   
               
               
                  2 
                 Ethanolamine → 
                 637213172- 
                 Aerobe 
                 Ethanolamine 
                 Oxygen  
                   
               
               
                   
                 Acetaldehyde → 
                 637213188 
                   
                 ammonia 
                 sensitivity 
                   
               
               
                   
                 Acetyl-CoA 
                   
                   
                 lyase (EutBC), 
                 product 
                   
               
               
                   
                   
                   
                   
                 acetaldehyde 
                 volatility/ 
                   
               
               
                   
                   
                   
                   
                 dehydrogenase 
                 toxicity 
                   
               
               
                   
                   
                   
                   
                 (EutE) 
                   
                   
               
               
                   
               
               
                  3 
                 1,2- 
                 637212757- 
                 Aerobe 
                 1,2- 
                 Oxygen 
                   
               
               
                   
                 propanediol → 
                 637212777 
                   
                 propanediol 
                 sensitivity 
                   
               
               
                   
                 proprionaldehyde → 
                   
                   
                 dehydratase 
                 product 
                   
               
               
                   
                 propanol 
                   
                   
                 (PduCDE), 
                 volatility/ 
                   
               
               
                   
                   
                   
                   
                 B12-dependent 
                 toxicity 
                   
               
               
                   
                   
                   
                   
                 propionaldehyde 
                   
                   
               
               
                   
                   
                   
                   
                 dehydrogenase 
                   
                   
               
               
                   
                   
                   
                   
                 (PduP) 
                   
                   
               
               
                   
               
               
                  4 
                 1,2- 
                 637924274- 
                 Generally 
                 Putative 1,2- 
                 Oxygen 
                   
               
               
                   
                 propanediol → 
                 637924291 
                 anaerobic 
                 propanediol 
                 sensitivity 
                   
               
               
                   
                 proprionaldehyde → 
                   
                 maybe 
                 dehydrogenase, 
                 product 
                   
               
               
                   
                 propanol 
                   
                 facultative 
                 B12-independent 
                 volatility/ 
                   
               
               
                   
                   
                   
                   
                 (GRE), 
                 toxicity 
                   
               
               
                   
                   
                   
                   
                 propionaldehyde 
                   
                   
               
               
                   
                   
                   
                   
                 dehydrogenase 
                   
                   
               
               
                   
                   
                   
                   
                 (PduP) 
                   
                   
               
               
                   
               
               
                  5 
                 Fuculose-1- 
                 641292279- 
                 Anaerobe 
                 Putative 1,2- 
                 Product 
                 A fusion of the B12-independent 
               
               
                   
                 phosphate → 
                 641292292 
                   
                 propanediol 
                 volatility/ 
                 1,2-propandiol dehydrogenase and 
               
               
                   
                 lactaldehyde → 
                   
                   
                 dehydrogenase, 
                 toxicity 
                 fuculose degradation pathways 
               
               
                   
                 1,2- 
                   
                   
                 B12-independent 
                   
                   
               
               
                   
                 propanediol → 
                   
                   
                 (GRE), 
                   
                   
               
               
                   
                 proprionaldehyde → 
                   
                   
                 propionaldehyde 
                   
                   
               
               
                   
                 propanol 
                   
                   
                 dehydrogenase 
                   
                   
               
               
                   
                   
                   
                   
                 (PduP) 
                   
                   
               
               
                   
                   
                   
                   
                 Fuculose-1- 
                   
                   
               
               
                   
                   
                   
                   
                 phosphate 
                   
                   
               
               
                   
                   
                   
                   
                 aldolase 
                   
                   
               
               
                   
                   
                   
                   
                 lactaldehyde 
                   
                   
               
               
                   
                   
                   
                   
                 oxidoreductase 
                   
                   
               
               
                   
               
               
                  6 
                 Ethanol → 
                 640858318- 
                 Anaerove: Can 
                 Aldehyde 
                 Product 
                 No nearby 03319 genes; Alcohol 
               
               
                   
                 Acetaldehyde → 
                 640858324 
                 grow on 
                 dehydrogenase; 
                 volatility/ 
                 dehydrogenases are probably 
               
               
                   
                 Acetyl-CoA 
                   
                 ethanol, 
                 alcohol 
                 toxicity 
                 encapsulated from experimental 
               
               
                   
                   
                   
                 acetate only 
                 dehydrogenase 
                   
                 evidence, but no obvious peptide like 
               
               
                   
                   
                   
                   
                   
                   
                 sequence found 
               
               
                   
               
               
                  7 
                 Fuculose-1- 
                 2501576836- 
                 Aerobe 
                 Fuculose-1- 
                 Product 
                   
               
               
                   
                 phosphate → 
                 2501576848 
                   
                 phosphate 
                 volatility/ 
                   
               
               
                   
                 lactaldehyde → 
                   
                   
                 aldolase 
                 toxicity 
                   
               
               
                   
                 ? 
                   
                   
                   
                   
                   
               
               
                   
               
               
                  8 
                 Fuculose-1- 
                 641690930- 
                 Obligate 
                 Fuculose/ 
                 Product 
                 Nearly identical to the enzymes found 
               
               
                   
                 phosphate or 
                 641690944 
                 anaerobe 
                 rhamnulose-1- 
                 volatility/ 
                 in Planctomycetes but also includes the 
               
               
                   
                 rhamnulose-1- 
                   
                   
                 phosphate 
                 toxicity 
                 rhamnulose degradation pathway  
               
               
                   
                 phosphate → 
                   
                   
                 aldolase; 
                   
                   
               
               
                   
                 lactaldehyde → 
                   
                   
                 aldehyde 
                   
                   
               
               
                   
                 lactate 
                   
                   
                 dehydrogenase 
                   
                   
               
               
                   
               
               
                  9 
                 Unknown; Highest 
                 641292513- 
                 Anaerobe 
                 Unknown glycyl 
                 Oxygen 
                   
               
               
                   
                 homology to 
                 641292533 
                   
                 radical with 
                 sensitivity 
                   
               
               
                   
                 glycerol 
                   
                   
                 homology to 
                 product 
                   
               
               
                   
                 dehydratase, but 
                   
                   
                 glycerol 
                 volatility/ 
                   
               
               
                   
                 not a GD 
                   
                   
                 dehydratase 
                 toxicity 
                   
               
               
                   
               
               
                 10 
                 L-aspartate-4- 
                 639738830- 
                 Aerobe, non 
                 Aldehyde 
                 Product 
                   
               
               
                   
                 semialdehyde or 
                 639738839 
                 pathogenic 
                 dehydrogenase; 
                 volatility/ 
                   
               
               
                   
                 glutamate-5- 
                   
                   
                 aminotransferase 
                 toxicity 
                   
               
               
                   
                 semialdehyde 
                   
                   
                 type III 
                   
                   
               
               
                   
                 based reactions 
                   
                   
                   
                   
                   
               
               
                   
               
               
                 11 
                 Homoserine &lt;--&gt; 
                 644016663- 
                 Aerobe 
                 L-homoserine: 
                 Product 
                   
               
               
                   
                 L-aspartate 4- 
                 644018672 
                   
                 NAD+ 
                 volatility/ 
                   
               
               
                   
                 semialdehyde &lt;--&gt; ? 
                   
                   
                 oxidoreductase 
                 toxicity 
                   
               
               
                   
                   
                   
                   
                 (not in BMC, in 
                   
                   
               
               
                   
                   
                   
                   
                 genome); 
                   
                   
               
               
                   
                   
                   
                   
                 dihydrodipicolinate 
                   
                   
               
               
                   
                   
                   
                   
                 synthase or other 
                   
                   
               
               
                   
                   
                   
                   
                 enzymes that 
                   
                   
               
               
                   
                   
                   
                   
                 function on 
                   
                   
               
               
                   
                   
                   
                   
                 L-aspartate-4- 
                   
                   
               
               
                   
                   
                   
                   
                 semialdehyde (not 
                   
                   
               
               
                   
                   
                   
                   
                 in BMC; in genome) 
                   
                   
               
               
                   
               
               
                 12 
                 N-acetyl- 
                 641050502- 
                 Aerotolerant 
                 N-acetyl- 
                 Product 
                 Contains entire glutamate-arginine 
               
               
                   
                 glutamylphosphate → 
                 641050513 
                 anaerobe; 
                 gammaglutamyl 
                 volatility/ 
                 conversion pathway; 2 00936 proteins, 
               
               
                   
                 N-acetylglutamate 
                   
                 pathogen 
                 phosphate 
                 toxicity 
                 no nearby 03319s 
               
               
                   
                 semialdehyde → 
                   
                   
                 reductase, 
                   
                   
               
               
                   
                 N-acetylornithine 
                   
                   
                 acetylornithine 
                   
                   
               
               
                   
                   
                   
                   
                 aminotransferase 
                   
                   
               
               
                   
               
               
                 13 
                 Hypoxanthine → 
                 640785432- 
                 Anaerobe 
                 Xanthine 
                 Xanthine  
                   
               
               
                   
                 xanthine → 
                 640785453 
                   
                 dehydrogenase; 
                 toxicity 
                   
               
               
                   
                 5-ureido-4- 
                   
                   
                 Xanthine hydrolase 
                   
                   
               
               
                   
                 imidazole 
                   
                   
                   
                   
                   
               
               
                   
                 carboxylate 
                   
                   
                   
                   
                   
               
               
                   
               
               
                 14 
                 Unknown  
                 640092924- 
                 Aerobe 
                 PduP/EutE aldehyde 
                 Product 
                   
               
               
                   
                 aldehyde 
                 640092931 
                   
                 dehydrogenase; 
                 volatility/ 
                   
               
               
                   
                 metabolism 
                   
                   
                 putative 
                 toxicity 
                   
               
               
                   
                   
                   
                   
                 glutathione 
                   
                   
               
               
                   
                   
                   
                   
                 dependent 
                   
                   
               
               
                   
                   
                   
                   
                 formaldeyde 
                   
                   
               
               
                   
                   
                   
                   
                 dehydrogenase 
                   
                   
               
               
                   
               
               
                 15 
                 Unknown 
                   
                 Anaerobic 
                 No readily apparent 
                 Unknown 
                 2 pfam00936, 3 pfam03319 scattered 
               
               
                   
                   
                   
                 photoautotrophic 
                 encapsulated 
                   
                 throughout genome 
               
               
                   
                   
                   
                   
                 enzymes near 
                   
                   
               
               
                   
                   
                   
                   
                 00936/03319 
                   
                   
               
               
                   
                   
                   
                   
                 proteins 
               
               
                   
               
            
           
         
       
     
     Shown another way, the present invention provides isolated consensus polypeptides in Table 3. For example, SEQ ID NO: 23 comprising: 
     
       
         
           
               
               
            
               
                   
                 (SEQ ID NO: 23) 
               
               
                   
                 X 1 X 2 GX 4 X 5 X 6 X 7 X 8 X 9 X 10 X 11 X 12 X 13 X 14 FPX 17 X 18   
               
            
           
         
       
     
     wherein: X 1  is V or I; X 2  is V or Y, X 4  is Q or K, X 5  is V, A, G or E, X 6  is Y, S or Q, X 7  is I, V, L or F, X 8  is N, Q, S or L, X 9  is K, Q or R, X 10  is M or L, X 11  L, M or R, X 12  is V, L, C or Q, X 13 , is T or 5, X 14  is L or M, X 17  is H, D or E and X 18  is R, N or Q. 
     Thus shown in another way, SEQ ID NO:25 is: 
     
       
         
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
             
               
                   
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 1 
                 1 
                 1 
                 1 
                 1 
                 1 
                 1 
               
               
                 Postn 1   
                 1 
                 2  
                 3  
                 4 
                 5 
                 6 
                 7 
                 8  
                 9 
                 0 
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
               
               
                   
               
             
            
               
                 AA(s) 
                 M 
                 D 
                 E 
                 K 
                 Q 
                 L 
                 K 
                 E 
                 I 
                 V 
                 R 
                 S 
                 V 
                 L 
                 A 
                 E 
               
               
                   
                   
                 N 
                 Q 
                 Q 
                 E 
                 I 
                 R 
                 D 
                 M 
                 I 
                 E 
                 Q 
                 I 
                 M 
                   
                 Q 
               
               
                   
                   
                   
                   
                   
                   
                   
                 E 
                   
                   
                   
                   
                   
                   
                   
                   
                 S 
               
               
                   
               
            
           
         
       
     
     In another embodiment, a targeting peptide is designed based on a consensus motif identified in the targeting peptides. Shown in an analysis of an alignment of all bacterial microcompartment targeting peptides ( FIG. 3B ), a distillation of the core amino acid properties (i.e. hydrophobic, polar, or charged) at each aligned position of the peptide was made based on the abundance of residues that fall into certain property groups at that position.  FIG. 3C  shows the amino acid percentage at each of the 17 well-aligned positions in the alignment of 305 unique bacterial microcompartment targeting peptides. Thus a consensus amino acid property can be assigned to each position. In the consensus motif shown in  FIG. 3C , majority amino acid percentages at each well-aligned position were calculated in JALVIEW. 
     Amino acid property at each position in the motif was given based on the majority amino acid property (H=hydrophobic, C=charged, P=polar) at each aligned position. Positions 5 and 15 were highly variable based on identity and property and no consensus property denoted by an X. Thus, the motif can be identified as: 
     Consensus Motif: H P C C X H C P H H C C H H X C H 
     where: 
     H=Hydrophobic Residues (Amino acids I, L, V, M, F, Y, A, W) 
     P=polar uncharged Residues (Amino acids Q, N, T, S, C) 
     C=Charged Residues (Amino acids D, E, R, K, H) 
     X=Any amino acid 
     Thus in one embodiment, the consensus motif allows one to design a targeting polypeptide. When mapped onto a helical wheel projection determined by a consensus of alpha helical secondary structure predictions of the peptides, one can create a consensus amphipathic helix for targeting bacterial microcompartments. For example, SEQ ID NO: 45 comprising: 
                            (SEQ ID NO: 45)           X 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 X 10 X 11 X 12 X 13 X 14 X 15 X 16 X 17              
wherein:
 
     X 1  is I, L, V, M, F, Y, A, or W; 
     X 2  is Q, N, T, S, or C, 
     X 3  is D, E, R, K, or H, 
     X 4  is D, E, R, K, or H, 
     X 5  is any residue, 
     X 6  is I, L, V, M, F, Y, A, or W, 
     X 7  is D, E, R, K, or H, 
     X 8  is Q, N, T, S, or C, 
     X 9  is I, L, V, M, F, Y, A, or W, 
     X 10  is I, L, V, M, F, Y, A, or W, 
     X 11  is D, E, R, K, or H, 
     X 12  is D, E, R, K, or H, 
     X 13 , is I, L, V, M, F, Y, A, or W, 
     X 14  is I, L, V, M, F, Y, A, or W, 
     X 15  is any residue, and 
     X 16  is D, E, R, K, or H, and 
     X 17  is I, L, V, M, F, Y, A, or W. 
     Thus shown in another way, SEQ ID NO:45 is: 
     
       
         
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
             
               
                   
               
               
                 Postn 1   
                 1 
                 2 
                 3 
                 4 
                 5 
                 6 
                 7 
                 8 
                 9 
                 10 
                 11 
                 12 
                 13 
                 14 
                 15 
                 16 
                 17 
               
               
                   
               
             
            
               
                 AA(s) 
                 I 
                 Q 
                 D 
                 D 
                 X 
                 I 
                 D 
                 Q 
                 I 
                 I 
                 D 
                 D 
                 I 
                 I 
                 X 
                 D 
                 I 
               
               
                   
                 L 
                 N 
                 E 
                 E 
                   
                 L 
                 E 
                 N 
                 L 
                 L 
                 E 
                 E 
                 L 
                 L 
                   
                 E 
                 L 
               
               
                   
                 V 
                 T 
                 R 
                 R 
                   
                 V 
                 R 
                 T 
                 V 
                 V 
                 R 
                 R 
                 V 
                 V 
                   
                 R 
                 V 
               
               
                   
                 M 
                 S 
                 K 
                 K 
                   
                 M 
                 K 
                 S 
                 M 
                 M 
                 K 
                 K 
                 M 
                 M 
                   
                 K 
                 M 
               
               
                   
                 F 
                 C 
                 H 
                 H 
                   
                 F 
                 H 
                 C 
                 F 
                 F 
                 H 
                 H 
                 F 
                 F 
                   
                 H 
                 F 
               
               
                   
                 Y 
                   
                   
                   
                   
                 Y 
                   
                   
                 Y 
                 Y 
                   
                   
                 Y 
                 Y 
                   
                   
                 Y 
               
               
                   
                 A 
                   
                   
                   
                   
                 A 
                   
                   
                 A 
                 A 
                   
                   
                 A 
                 A 
                   
                   
                 A 
               
               
                   
                   
                   
                   
                   
                   
                 W 
                   
                   
                 W 
                 W 
                   
                   
                 W 
                 W 
                   
                   
                 W 
               
               
                   
                 W 
               
               
                   
               
            
           
         
       
     
     In another embodiment, using the polypeptides of SEQ ID NOS: 1-192, a mechanism is provided for targeting biological molecules that would benefit from being compartmentalized and/or recombining them with other molecules and biological molecules within a bacterial microcompartment shell. This will enable the engineering of new or enhanced bacterial microcompartments. An example strategy is in one embodiment, a carboxysome shell protein is co-expressed with a fluorescent protein-peptide fusion. These protein-peptide fusions can be transferred among organisms (e.g. bacteria, fungi, plants, algae) using basic molecular techniques, followed by directed evolution to optimize phenotype. Alternatively, the modules are stable in solution or can be engineered to be (e.g., via reversible bonds/crosslinks), stable in solution, thus carrying out catalysis in cell free, non-biological systems. 
     In another embodiment, this allows one to engineer new metabolic modules (essentially organelles of specific function) into bacteria and it provides a new approach to designing and optimizing catalysis in solution. For example, insertion of polynucleotides encoding for the expression of the peptides provided for in SEQ ID NOS: 1-46, 145-190 or for example, at least the localization peptide regions in the polypeptides of SEQ ID NOS: 47-144 or 194-349. 
     In one embodiment, a bacterial microcompartment (BMC) and metabolic pathway is selected to be engineered. The polynucleotide encoding the bacterial compartment and enzymes in the metabolic pathway can be inserted into a host organism and if needed, expressed using an inducible expression system. The polynucleotide sequence encoding the peptides of SEQ ID NOS:1-192, 194-349, or a fragment thereof, can be inserted into the protein(s) in the N-terminus or C-terminus or between functional domains of the proteins, thereby permitting the encapsulation of the protein into the BMC upon expression. When referring to the bacterial compartments or microcompartments, it is meant to include any number of proteins, shell proteins or enzymes (e.g., dehydrogenases, aldolases, lyases, etc.) that comprise or are encapsulated in the compartment 
     In one embodiment, polynucleotides encoding a bacterial microcompartment shell proteins, and proteins containing a localization peptide (SEQ ID NOS: 1-192), are cloned into an appropriate plasmid under an inducible promoter, inserted into vector, and used to transform cells, such as  E. coli,  cyanobacteria, plants, algae, or other photosynthetic organisms. This system maintains the expression of the inserted gene silent unless an inducer molecule (e.g., IPTG) is added to the medium. 
     Bacterial colonies are allowed to grow after induction of gene expression. In one embodiment, the presently described peptides described in SEQ ID NOS: 1-192 are contemplated for use in any of the applications herein described. 
     In another embodiment, an expression vector comprising a nucleic acid sequence for a cluster of bacterial compartment genes and include a polynucleotide sequence which encodes any of the peptides of SEQ ID NOS:1-192 or a fragment thereof, which is then expressed in an organism by addition of an inducer molecule. 
     In some embodiments, expression cassettes comprising a promoter operably linked to a heterologous nucleotide sequence of the invention, i.e., any nucleotide sequence which encodes for a peptide comprising SEQ ID NOS:1-192 or a fragment thereof, that encodes a localization target sequence for microcompartment RNA or polypeptide are further provided. The expression cassettes of the invention find use in generating transformed plants, plant cells, microorganisms algae, fungi, and other eukaryotic organisms as is known in the art and described herein. The expression cassette will include 5′ and 3′ regulatory sequences operably linked to a polynucleotide of the invention. “Operably linked” is intended to mean a functional linkage between two or more elements. For example, an operable linkage between a polynucleotide of interest and a regulatory sequence (i.e., a promoter) is functional link that allows for expression of the polynucleotide of interest. Operably linked elements may be contiguous or non-contiguous. When used to refer to the joining of two protein coding regions, by operably linked is intended that the coding regions are in the same reading frame. The cassette may additionally contain at least one additional gene to be co-transformed into the organism. Alternatively, the additional gene(s) can be provided on multiple expression cassettes. Such an expression cassette is provided with a plurality of restriction sites and/or recombination sites for insertion of the polynucleotide that encodes a microcompartment RNA or polypeptide to be under the transcriptional regulation of the regulatory regions. The expression cassette may additionally contain selectable marker genes. 
     The expression cassette will include in the 5′-3′ direction of transcription, a transcriptional initiation region (i.e., a promoter), translational initiation region, a polynucleotide of the invention, a translational termination region and, optionally, a transcriptional termination region functional in the host organism. The regulatory regions (i.e., promoters, transcriptional regulatory regions, and translational termination regions) and/or the polynucleotide of the invention may be native/analogous to the host cell or to each other. Alternatively, the regulatory regions and/or the polynucleotide of the invention may be heterologous to the host cell or to each other. As used herein, “heterologous” in reference to a sequence that originates from a foreign species, or, if from the same species, is modified from its native form in composition and/or genomic locus by deliberate human intervention. For example, a promoter operably linked to a heterologous polynucleotide is from a species different from the species from which the polynucleotide was derived, or, if from the same/analogous species, one or both are substantially modified from their original form and/or genomic locus, or the promoter is not the native promoter for the operably linked polynucleotide. 
     Where appropriate, the polynucleotides may be optimized for increased expression in the transformed organism. For example, the polynucleotides can be synthesized using preferred codons for improved expression. 
     Additional sequence modifications are known to enhance gene expression in a cellular host. These include elimination of sequences encoding spurious polyadenylation signals, exon-intron splice site signals, transposon-like repeats, and other such well-characterized sequences that may be deleterious to gene expression. The G-C content of the sequence may be adjusted to levels average for a given cellular host, as calculated by reference to known genes expressed in the host cell. When possible, the sequence is modified to avoid predicted hairpin secondary mRNA structures. 
     The expression cassette can also comprise a selectable marker gene for the selection of transformed cells. Selectable marker genes are utilized for the selection of transformed cells or tissues. Marker genes include genes encoding antibiotic resistance, such as those encoding neomycin phosphotransferase II (NEO) and hygromycin phosphotransferase (HPT), as well as genes conferring resistance to herbicidal compounds, such as glufosinate ammonium, bromoxynil, imidazolinones, and 2,4-dichlorophenoxyacetate (2,4-D). Additional selectable markers include phenotypic markers such as β-galactosidase and fluorescent proteins such as green fluorescent protein (GFP) (Su et al. (2004)  Biotechnol Bioeng  85:610-9 and Fetter et al. (2004)  Plant Cell  6:215-28), cyan florescent protein (CYP) (Bolte et al. (2004)  J. Cell Science  117:943-54 and Kato et al. (2002)  Plant Physiol  129:913-42), and yellow florescent protein (PhiYFP™ from Evrogen, see, Bolte et al. (2004)  J. Cell Science  117:943-54). The above list of selectable marker genes is not meant to be limiting. Any selectable marker gene can be used in the present invention. 
     In another embodiment, it may be beneficial to express the gene from an inducible promoter, particularly from an inducible promoter. The gene product may also be co-expressed with a polypeptide comprising SEQ ID NOS: 1-192 or fragment thereof, such that the polypeptide is in the C-terminal or N-terminal region. 
     In one embodiment, an in-vitro transcription/translation system (e.g., Roche RTS 100  E. coli  HY) can be used to produce cell-free microcompartments or expression products which may be targeted by the polypeptides of the current invention. 
     In some embodiments, it is preferred that the microcompartments, comprising the microcompartment nucleic acids, proteins or polypeptides of the present invention described above, should provide an organism enhanced biomass production and CO 2  sequestration abilities, or produce valuable intermediates (Acetyl CoA), or sequester and protect oxygen-sensitive enzymes (engineered or native) or encapsulate reactions that would otherwise be toxic to the cell but however, be non-toxic or have low toxicity levels to humans, animals and plants or other organisms that are not the target. 
     The microcompartment proteins are preferably incorporated into a microorganism or eukaryote (plant, algae, yeast/fungi) to provide new or enhanced metabolic activity. In some embodiments, the microcompartment proteins are incorporated to provide enhanced carbon fixation and sequestration activity in the plant or organism (i.e., addition of a carboxysome) or produce valuable intermediates (Acetyl CoA), or sequester and protect oxygen-sensitive enzymes (engineered or native) or encapsulate reactions that would otherwise be toxic to the cell. 
     In another embodiment, a peptide of SEQ ID NO: 1-192 or fragment thereof, is used to target a biomolecule to a surface or a substrate. The peptides, which are derived from the targeting region of native BMC proteins and enzymes, appear to target the hexameric facets of BMC shell proteins. The biomolecule can be any native or modified protein, enzyme, cofactor, polymer, polysaccharide, polypeptide, or other biomolecule. 
     In another embodiment, when a surface comprising a BMC shell protein is made in vivo or in vitro, a peptide of SEQ ID NO:1-192 or fragment thereof, can be attached to a molecule or material whereby the peptide will localize the molecule or material to the surface of this molecular layer. It is contemplated that peptides SEQ ID NOS:1-192 or fragment thereof, can be used to tether any molecule or material to a substrate comprising a BMC shell protein. The substrate can be any shape or surface, such as a flat surface or molecular scaffold. 
     EXAMPLE 1 
     Identification of Consensus Sequence and Secondary Structure Prediction of Conserved C-Termini in Carboxysomal Protein, CcmN 
     Carboxysome protein, CcmN, and its orthologues from all β-cyanobacterial species were aligned and compared using MUSCLE (Edgar et al. (2004)  Nucleic Acids Research  32: 1792-97). For example, when visualized using Jalview (Waterhouse and Procter et al. (2009)  Bioinformatics  25: 1189-91), the consensus function built into the program produces SEQ ID NO:46, where the black bars represent percent identity. 

 
     The CcmN amino acid sequences from two of the most well studied β-cyanobacterial species,  Synechocystis  sp. PCC6803 and  Synechococcus elongatus  PCC7942, were analyzed using the Jpred 3 server (Cole et al. (2008)  Nucleic Acids Research  36: W197-W201), to determine the predicted secondary structure of the conserved C-termini of the proteins. The secondary structures for each protein are shown below, where the gray line represents a coil or loop motif, the black bar represents an alpha helical motif, and the light gray arrow represents a beta sheet motif. 

 
     EXAMPLE 2 
     Using a Targeting Peptide to Engineer New Metabolic Modules 
     One of the peptides of SEQ ID NOS:1-190 or a fragment thereof can be attached to the N-terminus or C-terminus (depending on where the peptide is natively found) or between domains of a protein to target that protein to shell proteins expressed in bacteria can be engineered, thus providing a new approach to designing and optimizing catalysis in solution. An example of using the CcmN peptide to target a fluorescent protein to the carboxysome in cyanobacteria is described (data not shown). A second example of the strategy for using the peptide to target a fluorescent protein to carboxysome shell proteins heterologously expressed in  E. coli  is also described (data not shown). 
       E. coli  cultures (strain BL21 DE3) were transformed with a plasmid containing the gene for the cyanobacterial carboxysome shell protein CcmK2 from  Synechococcus elongatus  PCC7942 (YP_400438) and co-transformed with a plasmid containing the gene for the cyanobacterial carboxysome shell protein CcmK3 and a plasmid containing a gene for Green Fluorescent Protein conjugated to the conserved targeting peptide sequence from CcmN of  S. elongatus  PCC7942 (18 C-terminal residues VYGKEQFLRMRQSMFPDR (SEQ ID NO: 191) with a GSGSGSGS linker (SEQ ID NO: 193) separating the GFP and peptide sequence). Plasmids were under lac repressor control. The cell cultures were grown to log phase (OD 0.6) at 37° C. and induced at 18° C. with 0.4 mM IPTG to express the shell proteins and GFP-target peptide conjugate. Cells were harvested after overnight induction fixed, embedded, and section using standard electron microscopy techniques. Thin sections were imaged on a Tecnai 12 microscope. High protein density regions were observed in many of the cells (image not shown) which is presumably from the expression of the carboxysome shell protein. The thin sections for the co-transformed culture were subsequently incubated with rabbit α-GFP antibodies as the primary antibody, washed, and then incubated with goat α-rabbit antibodies conjugated with gold particles. The immunolabeled sections were imaged to observed the presence of gold particles in the protein dense regions of the cell to show localization of the presumably shell protein (CcmK3) induced cellular substructure and the GFP-peptide conjugate (image not shown). 
     This is a way of bringing groups of enzymes that are functionally related into an organism or into solution. By delivering the enzymes to be encapsulated in a shell protein module, it is possible to introduce new functions that might otherwise be toxic to the cell, or incompatible with other aspects of cellular metabolism. Based on the design principles of naturally occurring metabolic modules, the naturally occurring assemblies of interior components and shell, we will be able to deliver groups of enzymes that are already (partially) optimized with respect to intermolecular interactions. 
     For example, many of the naturally occurring types of BMCs (Table 1) encapsulate reactions that produce toxic or volatile intermediates or encapsulate enzymes that are oxygen sensitive (e.g. RuBisCO). Other oxygen sensitive enzymes (e.g. nitrogenase) could be encapsulated in a BMC by attachment of the targeting signal to that enzyme and optimizing shell selectivity for nitrogenase-related metabolite flow by site-directed mutagenesis and directed/adaptive evolution. 
     Expression of shell proteins to self assemble into molecular layers and then targeting enzymes to the molecular layers using the peptide provides another example of how the targeting peptide can be used to attach proteins to a scaffold. Co-localization of functionally related enzymes in space, on a layer of shell proteins, can be used to enhance the overall rate of a series of enzymatic reactions. 
     In a second example, enzymes known to be targeted to BMCs could be used as a scaffold for new catalytic functionality. B12-independent diol dehydratase (a BMC encapsulated enzyme) is a homolog of pyruvate formate lyase (an enzyme not known to be encapsulated into a BMC) which produces the valuable metabolite Acetyl CoA. Pyruvate formate lyase is oxygen sensitive. Because of the homology between pyruvate formate lyase and B12-independent diol dehydratase a small number amino substitutions could be used to convert B12-independent diol dehydratase into pyruvate formate lyase. Concomitant modification of the shell selectivity properties could be used to create pyruvate formate lyase-containing BMCs that could be expressed in anaerobic organisms to produce the valuable metabolite acetyl-CoA. 
     EXAMPLE 3 
     Using a Targeting Peptide to Engineer New Metabolic Modules 
       Syenchococcus elongatus  PCC7942 was transformed with Yellow Fluorescent Protein (YFP) conjugated at the C-terminus to full-length CcmN (YP_400441) and under the native alphaphycocyanin promoter (papcA). The culture was grown under chloramphenicol selection at 30° C. in light. This was used as a positive control to show that carboxysome interior component CcmN is labeled with YFP. The image was captured at 100× magnification with a 3 second exposure time (YFP channel 513ex/530em) on a Zeiss AxioSkop 2 and was subsequently background subtracted using ImageJ software (Rasband, W. S., ImageJ, U.S. National Institutes of Health, Bethesda, Md., USA, http://rsb.info.nih.gov/ij/, 1997-2009.) The control indicates that CcmN is associated with the carboxysome gene cluster and contains the conserved peptide targeting sequence at its C-terminus. 
     A control experiment was then performed to show that CcmN and RuBisCO (RbcL) co-localize in a microcompartment.  Synechococcus elogatus  PCC7942 was co-transformed with a YFP-CcmN construct under the apcA promoter and the RuBisCO large subunit (RbcL) conjugated to Cyan Fluorescent Protein (CFP) at its C-terminus and under the ribosomal promoter prplC. The culture was grown under chloramphenicol and spectimnomycin selection at 30° C. in light. Images were captured at 100× magnification with a 3 second exposure time (513ex/530em) on a Zeiss Axioskop 2 and was subsequently background subtracted using ImageJ software, or at 100× magnification using a Applied Precision Deltavision Spectris DV4 deconvolution microscope. Each image was from the same z-plane taken at 500 ms exposure times using the YFP (513ex/530em) and CFP (433ex/475em) channels. The co-localization of fluorescence intensity provides a positive control for the localization of CcmN to the carboxysome since RbcL is known to localize to the carboxysome as well. 
     Syenchococcus elongatus PCC7942 was co-transformed with YFP conjugated with the linker region and the conserved targeting peptide from the C-terminus of CcmN [39 C-terminal residues from CcmN and identified as (132-VSSSEPAGRSPQSSAIAHPTKVYGKEQFLRMRQSMFPDR-160; SEQ ID NO:192)] and RbcL-CFP both under the rplC promoter. The culture was grown at 30° C. in light under chloramphenicol and spectinomycin selection. Images (not shown) were captured at 100× magnification with a 3 second exposure time (YFP channel 513ex/530em) on a Zeiss AxioSkop 2 and subsequently background subtracted using ImageJ software. Punctate fluorescence intensity was visible which is consistent with carboxysomal localization but the fluorescent signal was weak/undetectable in the CFP channel from the RbcL-CFP to provide conclusive evidence based on the co-localization of fluorescent signal. 
     In a second experiment,  Syenchococcus elongatus  PCC7942 was co-transformed with YFP conjugated to the linker region and conserved targeting peptide from the C-terminus of CcmN [39 C-terminal residues from CcmN (132-VSSSEPAGRSPQSSAIAHPTKVYGKEQFLRMRQSMFPDR-160; SEQ ID NO:192)] under the apcA promoter and RbcL-CFP under the rplC promoter. The culture was grown at 30° C. in light under chloramphenicol and spectinomycin selection. The images were captured at 100× magnification with a 3 second exposure time (YFP channel 513ex/530em) on a Zeiss AxioSkop 2 and subsequently background subtracted using ImageJ software. Again, punctate fluorescence intensity was visible which is consistent with carboxysomal localization but the fluorescent signal was weak/undetectable in the CFP channel from the RbcL-CFP to provide conclusive evidence based on the co-localization of fluorescent signal. 
     The above examples are provided to illustrate the invention but not to limit its scope. Other variants of the invention will be readily apparent to one of ordinary skill in the art and are encompassed by the appended claims. All publications, databases, and patents cited herein are hereby incorporated by reference for all purposes.