Patent Publication Number: US-10768170-B2

Title: Predictive biomarkers for CTLA-4 blockade therapy and for PD-1 blockade therapy

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a continuation of U.S. application Ser. No. 14/399,669, filed on Nov. 7, 2014, entitled “Predictive Biomarkers For CTLA-4 Blockade Therapy And For PD-1 Blockade Therapy,” which is a 371 application of International Application No. PCT/US2013/032254, filed Mar. 15, 2013, which claims benefit of U.S. Provisional Application No. 61/644,004, filed May 8, 2012, and U.S. Provisional Application No. 61/644,988, filed May 9, 2012, the disclosures of which are hereby incorporated by reference in their entirety. 
    
    
     STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT 
     This invention was made with Government Support under Grant No. R01 CA 109307 and Grant No. CA 129594 awarded by the National Institutes of Health (NIH). The Government has certain rights in the invention. 
    
    
     FIELD OF THE INVENTION 
     The invention is generally related to the field of predictive biomarkers, more particularly to biomarkers predictive for CTLA-4 blockade treatment and to biomarkers predictive for PD-1 blockade treatment. 
     BACKGROUND OF THE INVENTION 
     T cells play a pivotal role in anti-tumor responses, in the development of immune tolerance to self, and in autoimmunity. Cytotoxic T Lymphocyte-Associated antigen 4 (CTLA-4) is a surface receptor on T lymphocytes that down-regulates pathways of T-cell activation (Melero I, et al.  Nat Rev Cancer.  7:95-106 (2007)), serving as an immune check point molecule. It is expressed intracellularly in resting T cells, transported to the T cell surface after activation of the T cell receptor (TCR). TCR engagement leads to tyrosine phosphorylation of CTLA-4 via the SRC kinase and releases it from AP50, resulting in its surface expression within 48 hours of T cell activation, leading to T cell tolerance and ancrgy. CTLA-4 expression is associated with decreased proliferation with cell cycle arrest at the G 1 -S interface and diminished cytokine secretion (Chambers C A, et al.  Annu Rev Immunol.  19:565-94 (2001); Greenwald R J, et al.  Immunity.  14:145-55 (2001)). It decreases cell proliferation through the inhibition of mitogen-activated (MAP) kinases but promotes T cell survival through the binding of phosphoinositol-3 kinase and activating protein kinase B (PKB/AKT) resulting in T cell ancrgy and tolerance without T cell death (Schncider H, ct al.  PLoS One.  3:e3842 (2008)). CTLA-4 signals suppress both CD4 +  and CD8 +  T cell responses via a tyrosine-based inhibitory motif (Egen J G, et al.  Immunity.  16:23-35 (2002); Egen J G, et al.  Nat Immunol.  3:611-8 (2002)). 
     CTLA-4 blockade has antitumor activity in mice, and important effects on breaking tolerance (Egen J G, et al.  Nat Immunol.  3:611-8 (2002); Leach D R, et al.  Science.  271:1734-6 (1996); Quezada S A, et al.  J Clin Invest.  116:1935-45 (2006); Chen L, et al.  Cell.  71:1093-102 (1992); van Elsas A, et al.  J Exp Med.  190:355-66 (1999)). In experiments with B16 melanoma, a therapeutic effect induced by CTLA-4 blockade with a vaccine was associated with development of autoimmune vitiligo, suggesting that expansion of T cells recognizing melanocyte lineage antigens was associated with the therapeutic effect. Several autoimmune diseases were associated with single nucleotide polymorphisms in the CTLA-4 gene, including hypothyroidism and type I diabetes (Ueda H, et al.  Nature.  423:506-11 (2003)). In CTLA-4 −/−  knock-out mice, expansion of lymphocytes with diffuse lymphadenopathy and lymphoid infiltration of different organs occurs, consistent with a generalized expansion of T cells (Waterhouse P, et al.  Science.  270:985-8 (1995)). Similarly, both preclinical and clinical data indicate that CTLA-4 blockade results in activation and expansion of total CD4 |  and CD8 |  effector T cells (Wolchok J D, et al.  Oncologist.  13 Suppl 4:2-9 (2008)), and breaking of self-tolerance has been shown in patients, as evidenced by the occurrence of immune-related adverse events (irAEs) observed with two different CTLA-4 antibodies, ipilimumab (Bristol Myers Squibb, Princeton, N.J., USA) (Maker A V, et al.  J Immunol.  175:7746-54 (2005)) and tremelimumab (Pfizer, New York, N.Y., USA) (Ribas A, et al.  Oncologist.  13 Suppl 4:10-5 (2008)). 
     Ipilimumab, a fully human, CTLA-4 blocking lgG 1  monoclonal antibody induces long-lasting clinical responses in a minority of patients with metastatic melanoma (Wolchok J D, et al.  Lancet Oncol.  11:155-64 (2008); Weber J, et al.  Clin Cancer Res.  15:5591-8 (2009); Weber  J. Cancer Immunol Immunother.  58:823-30 (2009); Fong L, et al.  J Clin Oncol.  26:5275-83 (2008); Phan G Q, et al.  Proc Natl Acad Sci US A.  100:8372-7 (2003); O&#39;Day S J, et al.  Ann Oncol.  21:1712-7 (2010)). Ipilimumab, with or without a gp100 peptide vaccine, compared with gp100 vaccine alone, improved overall survival (OS) in patients with previously treated metastatic melanoma (Hodi F S, et al. NEngl  J Med.  363:711-23 (2010)). Ipilimumab combined with dacarbazine improved overall survival in previously untreated patients compared to dacarbazine alone (Robert C, et al.  NEngl J Med.  364:2517-26 (2011)). These were the first randomized Phase III trials to demonstrate a significant survival impact for patients with metastatic melanoma, yet few studies have documented pharmacodynamic markers of the impact of ipilimumab. An increase in the absolute lymphocyte count (ALC) after 2 or 3 doses of the drug at weeks 4 and 7 has been documented (Yuan J, et al.  Cancer Immun.  10:1 (2010)), and may correlate with an improved outcome; increased CD4 +  HLA-DR +  T cells have been shown in several studies to occur after ipilimumab therapy (Sanderson K, et al.  J Clin Oncol.  23:741-50 (2005); Sarnaik A A, et al.  Clin Cancer Res.  17(4):896-906 (2010)); in several small cohort studies of brief duration, ipilimumab treatment increased the frequency of CD4 +  ICOS hi  T cells in tumors and in the circulation, and also induced antibody reactivity against the cancer-testis antigen NY-ESO-1 Carthon B C, et al.  Clin Cancer Res.  16:2861-71 (2010); Chen H, et al.  Proc Natl Acad Sci US A.  106:2729-34 (2009)). CTLA-4 abrogating antibodies do not alter vaccine specific immune responses (Attia P, et al.  J Clin Oncol.  23:6043-53 (2005)) and even when administered with a peptide vaccine, tumor antigen specific responses were only modestly increased (Attia P, et al.  J Clin Oncol.  23:6043-53 (2005); Ribas A, et al.  Clin Cancer Res.  15:390-9 (2009)). Recall responses to viral and other antigens were not altered by ipilimumab. In patients receiving another CTLA-4 abrogating antibody, tremelimumab, the ratio of intratumoral T cells to FoxP3 positive T regulatory cells was increased after treatment only in regressing lesions (Ribas A, et al.  Clin Cancer Res.  15:390-9 (2009)), suggesting a therapeutic impact of CTLA-4 abrogation on T cells infiltrating the tumor. The same investigators also demonstrated that peripheral blood Th17 cells were induced by tremelimumab (von Euw E, et al.  J Transl Med.  7:35 (2009)), and that certain signaling pathways downstream of the TCR and cytokine receptor were also influenced by CTLA-4 blockade, such as increased pp38, pSTAT1 and pSTAT3, and decreased pLck, pERK1/2 and pSTAT5 levels (Comin-Anduix B, et al.  PLoS One.  5:e12711 (2010)). CTLA-4 blockade also induced cell proliferation in the spleen, a secondary lymphoid organ, shown by molecular imaging with the PET probe  18 F-FLT (Ribas A, et al.  J Nucl Med.  51:340-6 (2010)). They also reported significantly increased intratumoral CD8 |  T cell infiltration and CD4 |  T cells infiltration, demonstrated the activation of lymphocytes within tumor sites, as increase of HLA-DR and CD45RO double positive cells in post tremelimumab biopsies (Huang R R, et al.  Clin Cancer Res.  17:4101-9 (2011)) and increased expression of FoxP3. 
     To date, the precise molecular basis and mechanisms of action of ipilimumab have not been documented systematically in vivo. There is a critical need for biomarkers of the effects of ipilimumab as well as predictive biomarkers for clinical outcome and induction of irAE. 
     PD-1 blocking antibody (BMS-936558) therapy has shown antitumor activity and clinical benefit in melanoma patients. The precise molecular basis and mechanisms of PD-1 blockade in vivo have not been documented and there are few biomarker associated with clinical benefit. There is a critical need for biomarkers of the effects of PD-1 blockade treatment as well as predictive biomarkers for clinical outcome. 
     SUMMARY OF THE INVENTION 
     Biomarkers have been identified for predicting the efficacy, risk of relapse, risk of an immune related adverse event (irAE), or combination thereof for a CTLA-4 blockade treatment, such as ipilimumab, in a subject with a cancer, such as melanoma. Methods are therefore disclosed that involve assaying peripheral blood mononuclear cells (PBMCs) from a subject diagnosed with a cancer, such as a melanoma, for expression of one or more of the following biomarkcrs: CD8, Ki67, comcsodcrmin (EOMES), transforming growth factor beta receptor III (TGFβR3), C—C chemokine receptor type 7 (CCR7), CD4, CD71, and CD109. For example, expression can be assayed for one or more of the following biomarkers: CD8, Ki67, eomesodermin (EOMES), transforming growth factor beta receptor III (TGFβR3), and C—C chemokine receptor type 7 (CCR7). For example, the method can involve assaying the PBMCs for the presence of (a) CD8, Ki67, and EOMES; (b) CD8 and EOMES; (c) CD8 and TGFβR3; (d) CD4 and CCR7; (e) CD4, Ki67, and EOMES; (f) CD4 and CD71; or (g) CD8 and CD109. For example, the method can involve assaying the PBMCs for the presence of (a) CD8, Ki67, and EOMES; (b) CD8 and EOMES; (c) CD8 and TGFβR3; (d) CD4 and CCR7; or (e) CD4, Ki67, and EOMES. 
     Also disclosed are methods of treating a subject diagnosed with melanoma with a CTLA-4 blockade treatment if the expression level of one or more of the biomarkers listed in  FIGS. 11, 12, 13, and 14  are at or beyond a threshold level, such as a level that is greater than or less than (depending on the marker) a reference level. For example, the ratio of responder (NED or non-irAE) to nonresponder (Relapse or irAE) can be used as, or to define a threshold level or a reference level. 
     Also disclosed are methods of not treating a subject with a CTLA-4 blockade treatment if the expression level of one or more of the biomarkers listed in Tables 10, 11, 12, and 13 are at or beyond a threshold level, such as a level that is greater than or less than (depending on the marker) a reference level. For example, the ratio of responder (NED or non-irAE) to nonresponder (Relapse or irAE) can be used as, or to define a threshold level or a reference level. 
     In some embodiments, the frequency of Ki67 + EOMES + CD8 +  T cells in CD8 +  T cells in the PBMCs is inversely associated with the risk of relapse after CTLA-4 blockade treatment. For example, a frequency of Ki67 + EOMES + CD8 +  T cells in CD8 +  T cells in the PBMCs less than about 2.11%, preferably less than about 2.0%, more preferably less than about 1.8%, most preferably less than about 1.6%, is an indication that the subject is at risk of relapse after CTLA-4 blockade treatment. Likewise, a frequency of Ki67 + EOMES + CD8 +  T cells in CD8 +  T cells in the PBMCs greater than about 2.2%, preferably greater than about 2.3%, more preferably greater than about 2.4%, most preferably greater than about 2.5%, is an indication that the subject will have relapse free survival after CTLA-4 blockade treatment. 
     In some embodiments, the frequency of EOMES + CD8 +  T cells in CD8 +  T cells in the PBMCs is inversely associated with the risk of relapse after CTLA-4 blockade treatment. For example, a frequency of EOMES + CD8 +  T cells in CD8 +  T cells in the PBMCs less than about 55.6%, preferably less than about 50%, more preferably less than about 45%, most preferably less than about 40%, is an indication that the subject is at risk of relapse after CTLA-4 blockade treatment. Likewise, a frequency of EOMES + CD8 +  T cells in CD8 +  T cells in the PBMCs greater than about 56%, preferably greater than about 57%, more preferably greater than about 59%, most preferably greater than about 61% is an indication that the subject will have relapse free survival after CTLA-4 blockade treatment. 
     In some embodiments, the expression level of CCR7 on CD4 +  T cells in the PBMCs is directly associated with the risk of relapse after CTLA-4 blockade treatment. For example, an expression level of CCR7 on CD4 +  T cells in the PBMCs greater than about 2402, preferably greater than about 2500, more preferably greater than about 2700, most preferably greater than about 2900, is an indication that the subject is at risk of relapse after CTLA-4 blockade treatment. Likewise, an expression level of CCR7 on CD4 +  T cells in the PBMCs less than about 2400, preferably less than about 2300, more preferably less than about 2100, most preferably less than about 1900, is an indication that the subject will have relapse free survival after CTLA-4 blockade treatment. 
     In some embodiments, the frequency of Ki67 + EOMES + CD4 +  T cells in CD4 +  T cells in the PBMCs is inversely associated with the risk of an immune related adverse event (irAE) after CTLA-4 blockade treatment. For example, a frequency of Ki67 + EOMES + CD4 +  T cells in CD4 +  T cells in the PBMCs less than about 0.446%, preferably less than about 0.4%, more preferably less than about 0.35%, most preferably less than about 0.3%, is an indication that the subject is at risk of an immune related adverse event (irAE) after CTLA-4 blockade treatment. Likewise, a frequency of Ki67 + EOMES + CD4 +  T cells in CD4 +  T cells in the PBMCs greater than about 0.45%, preferably greater than about 0.5%, more preferably greater than about 0.6%, most preferably greater than about 0.7%, is an indication that the subject will not have an irAE after CTLA-4 blockade treatment. 
     In some embodiments, the expression level of TGFβR3 on CD8 +  T cells in the PBMCs is directly associated with risk of an immune related adverse event (irAE) after CTLA-4 blockade treatment. For example, an expression level of TGFβR3 on CD8 +  T cells in the PBMCs greater than about 527, preferably greater than about 600, more preferably greater than about 700, most preferably greater than about 1000, is an indication that the subject is at risk of an immune related adverse event (irAE) after CTLA-4 blockade treatment. Likewise, an expression level of TGFβR3 on CD8 +  T cells in the PBMCs less than about 525, preferably less than about 500, more preferably less than about 450, most preferably less than about 400, is an indication that the subject will not have an immune related adverse event (irAE) after CTLA-4 blockade treatment. 
     In some embodiments, the expression level of CD71 on CD4 +  T cells in the PBMCs is inversely associated with risk of an immune related adverse event (irAE) after CTLA-4 blockade treatment. 
     In some embodiments, the frequency of CD109 + CD8 +  T cells in CD8 +  T cells in the PBMCs is directly associated with the risk of relapse after CTLA-4 blockade treatment. For example, a frequency of CD109 + CD8 +  T cells in CD8 +  T cells in the PBMCs greater than about 0.7315%, preferably greater than about 0.8%, more preferably greater than about 1.0%, most preferably greater than about 2.0%, is an indication that the subject is at risk of relapse after CTLA-4 blockade treatment. Likewise, a frequency of CD109 + CD8 +  T cells in CD8 +  T cells in the PBMCs less than about 0.7316%, preferably less than about 0.7%, more preferably less than about 0.6%, most preferably less than about 0.5% is an indication that the subject will have relapse free survival after CTLA-4 blockade treatment. 
     In some embodiments, the frequency of CD71 + CD4 +  T cells in CD4 +  T cells in the PBMCs is inversely associated with the risk of an immune related adverse event (irAE) after CTLA-4 blockade treatment. For example, a frequency of CD71 + CD4 +  T cells in CD4 +  T cells in the PBMCs less than about 2.80%, preferably less than about 2.5%, more preferably less than about 2.0%, most preferably less than about 1.5%, is an indication that the subject is at risk of an immune related adverse event (irAE) after CTLA-4 blockade treatment. Likewise, a frequency of CD71 + CD4 +  T cells in CD4 +  T cells in the PBMCs greater than about 2.8%, preferably greater than about 3.0%, more preferably greater than about 3.5%, most preferably greater than about 4%, is an indication that the subject will not have an irAE after CTLA-4 blockade treatment. 
     The disclosed method can further involve using the biomarkers to select subjects suitable for treatment with a CTLA-4 blockade agent, such as ipilimumab or tremelimumab, or a treatment other than a CTLA-4 blockade treatment, such as a neoplastic agent, or a combination of CTLA-4 blockade agent and another treatment. 
     For example, in some embodiments, the method involves selecting a subject for CTLA-4 blockade treatment, another treatment, or a combination thereof if (a) if the frequency of Ki67 + EOMES + CD8 +  T cells in the subject&#39;s CD8 +  T cells is at least about 2.2%, preferably at least about 2.3%, more preferably at least about 2.4%, most preferably at least about 2.5%; (b) if the frequency of EOMES + CD8 +  T cells in the subject&#39;s CD8 +  T cells is at least 56%, preferably at least about 57%, more preferably at least about 59%, most preferably at least about 61%; (c) if the expression level of CCR7 on CD4 +  T cells in the subject is lower than about 2400, preferably lower than about 2300, more preferably lower than about 2100, most preferably lower than about 1900; (d) if the frequency of Ki67 + EOMES + CD4 +  T cells in the subject&#39;s CD4 +  T cells is at least about 0.45%, preferably at least about 0.5%, more preferably at least about 0.6%, most preferably at least about 0.7%; (e) if the expression level of TGFβR3 on CD8 +  T cells in the subject is lower than about 525; preferably lower than about 500, more preferably lower than about 450, most preferably lower than about 400; (f) the expression level of CD109 on CD8 +  T cells is less than 0.7316%, preferably less than about 0.7%, more preferably less than about 0.6%, most preferably less than about 0.5%; (g) the frequency of CD71 + CD4 +  T cells in CD4 +  T cells in the PBMCs is at least 2.80%, preferably greater than about 3.0%, more preferably greater than about 3.5%, most preferably greater than about 4%; or any combination thereof. 
     Therefore, in these embodiments, the subject is not treated with CTLA-4 blockade treatment, is treated with another treatment, or a combination thereof if (a) the frequency of Ki67 + EOMES + CD8 +  T cells is less than about 2.11%, preferably less than about 2.0%, more preferably less than about 1.8%, most preferably less than about 1.6%; (b) the frequency of EOMES + CD8 +  T cells is less than about 55.6%, preferably less than about 50%, more preferably less than about 45%, most preferably less than about 40%; (c) the expression level of CCR7 on CD4 +  T cells is greater than about 2402, preferably greater than about 2500, more preferably greater than about 2700, most preferably greater than about 2900; (d) the frequency of Ki67 + EOMES + CD4 +  T cells is less than about 0.446%, preferably less than about 0.4%, more preferably less than about 0.35%, most preferably less than about 0.3%; (e) the expression level of TGFβR3 on CD8 +  T cells is greater than about 527, preferably greater than about 600, more preferably greater than about 700, most preferably greater than about 1000; (f) the frequency of CD109 + CD8 +  T cells in CD8 +  T cells is greater than about 0.7315%, preferably greater than about 0.8%, more preferably greater than about 1.0%, most preferably greater than about 2.0%; (g) the frequency of CD71 + CD4 +  T cells in CD4 +  T cells is less than about 2.80%/a, preferably less than about 2.5%, more preferably less than about 2.0%, most preferably less than about 1.5% or any combination thereof. 
     Methods of treating subjects diagnosed with melanoma are also disclosed that involve treating the subject with a CTLA-4 blockade treatment, another treatment, or a combination thereof (a) if the frequency of Ki67 + EOMES + CD8 +  T cells in the subject&#39;s CD8 +  T cells is at least about 2.2%, preferably at least about 2.3%, more preferably at least about 2.4%, most preferably at least about 2.5%; (b) if the frequency of EOMES + CD8 +  T cells in the subject&#39;s CD8 +  T cells is at least 56%, preferably at least about 57%, more preferably at least about 59%; (c) if the expression level of CCR7 on CD4 +  T cells in the subject is lower than about 2400, preferably lower than about 2300, more preferably lower than about 2100, most preferably lower than about 1900; (d) if the frequency of Ki67 + EOMES + CD4 +  T cells in the subject&#39;s CD4 +  T cells is at least about 0.45%, preferably at least about 0.5%, more preferably at lcast about 0.6%, most preferably at least about 0.7%; (e) if the expression level of TGFβR3 on CD8 +  T cells in the subject is lower than about 525; preferably lower than about 500, more preferably lower than about 450, most preferably lower than about 400; (f) the expression level of CD109 on CD8 +  T cells is less than 0.7316%, preferably less than about 0.7%, more preferably less than about 0.6%, most preferably less than about 0.5%; (g) the frequency of CD71 + CD4 +  T cells in CD4 +  T cells in the PBMCs is at least 2.80%, preferably greater than about 3.0%, more preferably greater than about 3.5%, most preferably greater than about 4%; or any combination thereof. 
     Biomarkers have been identified for predicting the efficacy and clinical benefit for a PD-1 blockade treatment, such as a PD-1 blocking antibody, in a subject with a cancer, such as melanoma. Methods are therefore disclosed that involve assaying peripheral blood mononuclear cells (PBMCs) from a subject diagnosed with a cancer, such as a melanoma, for expression of one or more of the biomarkers listed in Tables 8 and 9. 
     Disclosed are methods comprising assaying peripheral blood mononuclear cells (PBMCs) from a subject diagnosed with melanoma for expression of CD8 and one or a combination of markers in Tables 8 and 9, where, depending on the marker, the level of expression of certain markers on CD8 +  T cells in the PBMCs of the subject prior to PD-1 blockade treatment or a change in the expression level of certain markers on CD8 +  T cells in the PBMCs following PD-1 blockade treatment indicate the expected clinical benefit of PD-1 blockade treatment of the subject. 
     Also disclosed are methods of treating a subject diagnosed with melanoma with a PD-1 blockade treatment if the expression level of one or more of the biomarkers listed in Tables 8 and 9 are at or beyond a threshold level, such as a level that is greater than or less than (depending on the marker) a reference level. 
     Also disclosed are methods of treating a subject diagnosed with melanoma with a treatment other than a PD-1 blockade treatment, or with both a PD-1 blockade treatment and a different treatment, if the expression level of one or more of the biomarkers listed in Tables 8 and 9 are at or beyond a threshold level, such as a level that is greater than or less than (depending on the marker) a reference level. 
     In some forms of the methods, the PBMCs can be assayed prior to PD-1 blockade treatment of the subject. Expression levels measured prior to PD-1 blockade treatment can be referred to as baseline expression. In some forms of the methods, the expression prior the PD-1 blockade treatment is compared to a reference level of expression. 
     In some forms of the methods, the PBMCs can also be assayed following PD-1 blockade treatment of the subject. Expression following the PD-1 blockade treatment can be compared to the expression prior to the PD-1 blockade treatment. Depending on the marker, a change in the expression level of certain markers on CD8 +  T cells in the PBMCs following PD-1 blockade treatment indicate the expected clinical benefit of PD-1 blockade treatment of the subject. In some forms, a change in expression level can indicate that PD-1 blockade treatment should be continued with the subject. 
     Also disclosed are methods of selecting a subject for PD-1 blockade treatment if the expression level of one or more of the biomarkers listed in Tables 8 and 9 are at or beyond a threshold level, such as a level that is greater than or less than (depending on the marker) a reference level. 
     Also disclosed are methods of not treating a subject with a PD-1 blockade treatment if the expression level of one or more of the biomarkers listed in Tables 8 and 9 are at or beyond a threshold level, such as a level that is greater than or less than (depending on the marker) a reference level. 
     In some forms of the methods, the PD-1 blockade treatment can be treatment with a PD-1 blocking antibody. In some forms of the methods, the PD-1 blockade treatment can be treatment with PD-1 blocking antibody BMS-936558 or BMS-936559. 
     For example, expression of one or a combination of Phosphatase and tensin homolog (PTEN), GATA zinc finger domain containing 1 (GATAD1), Natural killer-tumor recognition sequence (NKTR), CD276 molecule (B7-H3) (CD276), NOL1/NOP2/Sun domain family, member 6 (NSUN6), Cell division cycle and apoptosis regulator 1 (CCAR1), SET and MYND domain containing 2 (SMYD2), F-box protein 9 (FBXO9), Non-protein coding RNA 153(NCRNA00153), Neutral sphingomyelinase activation associated factor (NSMAF), Pyrimidinergic receptor P2Y, G-protein coupled, 4 (P2RY4), REST corcprcssor 2 (RCOR2), Negative regulator of ubiquitin-like proteins 1 (NUB1), Fc receptor-like 2 (FCRL2), Nuclear casein kinase, cyclin-dependent kinase substrate 1 (NUCKS1), SHC transforming protein 2 (SHC2), Stearoyl-CoA desaturase 5 (SCD5), Ubiquitin specific peptidase 9, Y-linked (USP9Y), Heat shock 60 kDa protein 1 (chaperonin) (HSPD1), Cytokine inducible SH2-containing protein (CISH), Kruppel-like factor 12 (KLF12), Neutrophil cytosolic factor 4, 40 kDa (NCF4), Splicing factor, arginine/serine-rich 7, 35 kDa (SFRS7), CDC14 cell division cycle 14 homolog A ( S. cerevisiae ) (CDC14A), Mitogen-activated protein kinase kinase 5 (MAP2K5), Interleukin 11 receptor, alpha (IL11RA), Myeloid/lymphoid or mixed-lineage leukemia (MLL), HSPB (heat shock 27 kDa) associated protein 1 (HSPBAP1), Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), Helicase, lymphoid-specific (HELLS), C1q and tumor necrosis factor related protein 3 (C1QTNF3), Ubiquitin-conjugating enzyme E2D 1 (UBE2D1), Fas apoptotic inhibitory molecule 3 (FAIM3), Interferon-induced protein 44 (IFI44), H2B histone family, member M (H2BFM), GATA zinc finger domain containing 1 (GATAD1), Caspase 8, apoptosis-related cysteine peptidase (CASP8), Suppressor of cytokine signaling 1 (SOCS1), Programmed cell death 6 (PDCD6), LAG1 homolog, ceramide synthase 6 (LASS6), Growth arrest DNA-damage-inducible, beta (GADD45B), Early growth response 2 (EGR2), Early growth response 1 (EGR1), Growth arrest, DNA-damage-inducible, g (GADD45G), CDC28 protein kinase regulatory subunit 2 (CKS2), Cyclin-dependent kinase inhibitor 1C (CDKN1C), Immediate early response 5 (IER5), Inhibitor of growth family, member 1 (ING1), Interferon induced with helicase C domain 1 (IFIH1), Interferon, gamma (IFNG), Immediate early response 5-like (IER5L), Cancer/testis antigen 1 (NY-ESO-1), and Melanoma antigen recognized by T-cells 1 (MART-1) can be detected, measured, assayed, assessed, etc. 
     The levels of expression of the markers can indicate the efficacy, predicted efficacy, likelihood of clinical benefit, prognosis, etc., of a subject treated with a PD-1 blockade treatment. For example, the expression level of PTEN on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of GATAD1 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NKTR on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CD276 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NSUN6 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CCAR1 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SMYD2 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of FBXO9 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NCRNA00153 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NSMAF on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of P2RY4 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of RCOR2 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NUB1 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of FCRL2 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NUCKS1 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SHC2 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SCD5 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of USP9Y on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of HSPD1 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CISH on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of KLF12 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NCF4 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SFRS7 on CD8 |  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CDC14A on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of MAP2K5 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of IL11RA on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of MLL on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of HSPBAP1 on CD8 |  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of MAP4K4 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of HELLS on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of C1QTNF3 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of UBE2D1 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of FAIM3 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of IFI44 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of H2BFM on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of GATAD1 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CASP8 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SOCS1 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of PDCD6 on CD8 +  T cells in the PBMCs is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NY-ESO-1 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of MART-1 on CD8 +  T cells in the PBMCs is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; an increase in the expression level of LASS6 on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of GADD45B on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of EGR2 on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of EGR1 on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of GADD45G on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of CKS2 on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of CDKN1C on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of IER5 on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of ING1 on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of IFIH1 on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of IFNG on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the expression level of IER5L on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; an increase in the expression level of NY-ESO-1 on CD8 +  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; an increase in the expression level of MART-1 on CD8 |  T cells in the PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the number or frequency of T regulatory cells (Tregs; characterized as FoxP3 + CD127 Low  CD25 + CD4 + ) in CD4 +  T cells in PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; an increase in the number or frequency of T regulatory cells (Tregs; characterized as FoxP3 + CD127 Low CD25 + CD4 + ) in CD4 +  T cells in PBMCs following PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the number or frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells in PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; an increase in the number or frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells in PBMCs following PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; a decrease in the number or frequency of CTLA-4 + CD8 +  cells in CD8 +  T cells in PBMCs following PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; an increase in the number or frequency of CTLA-4 + CD8 +  cells in CD8 +  T cells in PBMCs following PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject. 
     As another example, disclosed are methods of treating a subject diagnosed with melanoma with a PD-1 blockade treatment if the expression level of PTEN on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is more than 1.2 times, preferably more than 1.3 times, most preferably more than 1.4 times the reference level of PTEN; the expression level of GATAD1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.1 times, preferably more than 1.3 times, most preferably more than 1.4 times the reference level of GATAD1; the expression level of NKTR on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.1 times, preferably more than 1.3 times, most preferably more than 1.4 times the reference level of NKTR; the expression level of CD276 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 0.9 times, preferably less than 0.8 times, most preferably less than 0.7 times the reference level of CD276; the expression level of NSUN6 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.4 times, preferably more than 1.7 times, most preferably more than 1.9 times the reference level of NSUN6; the expression level of CCAR1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.2 times, preferably more than 1.5 times, most preferably more than 1.7 times the reference level of CCAR1; the expression level of SMYD2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.2 times, preferably more than 1.4 times, most preferably more than 1.6 times the reference level of SMYD2; the expression level of FBXO9 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.2 times, preferably more than 1.3 times, most preferably more than 1.4 times the reference level of FBXO9; the expression level of NCRNA00153 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.2 times, preferably more than 1.3 times, most preferably more than 1.4 times the reference level of NCRNA00153; the expression level of NSMAF on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.2 times, preferably more than 1.3 times, most preferably more than 1.4 times the reference level of NSMAF; the expression level of P2RY4 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 0.9 times, preferably less than 0.8 times, most preferably less than 0.7 times the reference level of P2RY4; the expression level of RCOR2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 0.9 times, preferably less than 0.8 times, most preferably less than 0.7 times the reference level of RCOR2; the expression level of NUB1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 0.9 times, preferably less than 0.8 times, most preferably less than 0.7 times the reference level of NUB1; the expression level of FCRL2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 0.9 times, preferably less than 0.7 times, most preferably less than 0.5 times the reference level of FCRL2; the expression level of NUCKS1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 0.8 times, preferably less than 0.7 times, most preferably less than 0.6 times the reference level of NUCKS1; the expression level of SHC2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 0.8 times, preferably less than 0.7 times, most preferably less than 0.6 times the reference level of SHC2; the expression level of SCD5 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 0.8 times, preferably less than 0.6 times, most preferably less than 0.4 times the reference level of SCD5; the frequency of NY-ESO-1 + CD8 +  cells in CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is less than 0.5%, preferably less than 0.4%, most preferably less than 0.3%; the frequency of MART-1 + CD8 +  cells in CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is less than 0.5%, preferably less than 0.4%, most preferably less than 0.3%; or a combination. The subject can also expect a clinical benefit from PD-1 blockade treatment at these levels of expression. 
     As another example, disclosed are methods of treating a subject diagnosed with melanoma with a PD-1 blockade treatment if the expression level of LASS6 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is more than 1.2 times, preferably more than 1.3 times, most preferably more than 1.4 times the expression level prior to the PD-1 blockade treatment; the expression level of GADD45B on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.8 times, preferably less than 0.7 times, most preferably less than 0.6 times the expression level prior to the PD-1 blockade treatment; the expression level of EGR2 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.8 times, preferably less than 0.7 times, most preferably less than 0.6 times the expression level prior to the PD-1 blockade treatment; the expression level of EGR1 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.7 times, preferably less than 0.4 times, most preferably less than 0.2 times the expression level prior to the PD-1 blockade treatment; the expression level of GADD45G on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.9 times, preferably less than 0.8 times, most preferably less than 0.7 times the expression level prior to the PD-1 blockade treatment; the expression level of CKS2 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.9 times, preferably less than 0.8 times, most preferably less than 0.7 times the expression level prior to the PD-1 blockade treatment; the expression level of CDKN1C on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.9 times, preferably less than 0.8 times, most preferably less than 0.7 times the expression level prior to the PD-1 blockade treatment; the expression level of IER5 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.9 times, preferably less than 0.8 times, most preferably less than 0.7 times the expression level prior to the PD-1 blockade treatment; the frequency of NY-ESO-1 + CD8 +  cells in CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.7 times, preferably less than 0.6 times, most preferably less than 0.5 times the frequency prior to the PD-1 blockade treatment; the frequency of MART-1 + CD8 +  cells in CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 0.7 times, preferably less than 0.6 times, most preferably less than 0.5 times the frequency prior to the PD-1 blockade treatment; the number or frequency of T regulatory cells (Tregs; characterized as FoxP3 + CD127 Low CD25 + CD4 + ) in CD4 +  T cells in PBMCs following the PD-1 blockade treatment is less than 1.0 times, preferably less than 0.9 times, most preferably less than 0.8 times the number or frequency of Tregs in CD4 +  T cells in PBMCs prior to the PD-1 blockade treatment; the number or frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells in PBMCs following the PD-1 blockade treatment is less than 1.0 times, preferably less than 0.9 times, most preferably less than 0.8 times the number or frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells in PBMCs prior to the PD-1 blockade treatment; the number or frequency of CTLA-4 + CD8 +  cells in CD8 +  T cells in PBMCs following the PD-1 blockade treatment is less than 1.0 times, preferably less than 0.9 times, most preferably less than 0.8 times the number or frequency of CTLA-4 + CD8 +  cells in CD8 +  T cells in PBMCs prior to the PD-1 blockade treatment; or a combination. 
     As another example, disclosed are methods of treating a subject diagnosed with melanoma with a treatment other than a PD-1 blockade treatment, or with both a PD-1 blockade treatment and a different treatment, if the expression level of PTEN on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is less than 1.1 times, preferably less than 1.0 times, most preferably less than 0.9 times the reference level of PTEN; the expression level of GATAD1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 1.0 times, preferably less than 0.9 times, most preferably less than 0.8 times the reference level of GATAD1; the expression level of NKTR on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 1.0 times, preferably less than 0.9 times, most preferably less than 0.8 times the reference level of NKTR; the expression level of CD276 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the reference level of CD276; the expression level of NSUN6 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 1.2 times, preferably less than 1.0 times, most preferably less than 0.8 times the reference level of NSUN6; the expression level of CCAR1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 1.1 times, preferably less than 1.0 times, most preferably less than 0.9 times the reference level of CCAR1; the expression level of SMYD2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 1.1 times, preferably less than 1.0 times, most preferably less than 0.9 times the reference level of SMYD2; the expression level of FBXO9 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 1.1 times, preferably less than 1.0 times, most preferably less than 0.9 times the reference level of FBXO9; the expression level of NCRNA00153 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 1.1 times, preferably less than 1.0 times, most preferably less than 0.9 times the reference level of NCRNA00153; the expression level of NSMAF on CD8 +  T cells in the PBMCs prior to PD-1 blockade is less than 1.1 times, preferably less than 1.0 times, most preferably less than 0.9 times the reference level of NSMAF; the expression level of P2RY4 on CD8 |  T cells in the PBMCs prior to PD-1 blockade is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the reference level of P2RY4; the expression level of RCOR2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the reference level of RCOR2; the expression level of NUB1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the reference level of NUB1; the expression level of FCRL2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the reference level of FCRL2; the expression level of NUCKS1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 0.9 times, preferably more than 1.0 times, most preferably more than 1.1 times the reference level of NUCKS1; the expression level of SHC2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 0.9 times, preferably more than 1.0 times, most preferably more than 1.1 times the reference level of SHC2; the expression level of SCD5 on CD8 +  T cells in the PBMCs prior to PD-1 blockade is more than 0.9 times, preferably more than 1.0 times, most preferably more than 1.1 times the reference level of SCD5; the frequency of NY-ESO-1 + CD8 +  cells in CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is more than 0.5%, preferably more than 0.7%, most preferably more than 0.8%; the frequency of MART-1 + CD8 +  cells in CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is more than 0.5%, preferably more than 0.7/o, most preferably more than 0.8%; the number or frequency of Tregs in CD4 +  T cells in PBMCs following PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the number or frequency of Tregs in CD4 +  T cells in PBMCs prior to the PD-1 blockade treatment; the number or frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells in PBMCs following the PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the number or frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells in PBMCs prior to the PD-1 blockade treatment; the number or frequency of CTLA-4 + CD8 +  cells in CD8 +  T cells in PBMCs following the PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the number or frequency of CTLA-4 + CD8 +  cells in CD8 +  T cells in PBMCs prior to the PD-1 blockade treatment; or a combination. The subject can also expect a clinical benefit from PD-1 blockade treatment at these levels of expression. 
     As another example, disclosed are methods of treating a subject diagnosed with melanoma with a treatment other than a PD-1 blockade treatment, or with both a PD-1 blockade treatment and a different treatment, if the expression level of LASS6 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is less than 1.1 times, preferably less than 1.0 times, most preferably less than 0.9 times the expression level prior to the PD-1 blockade treatment; the expression level of GADD45B on CD8 |  T cells in the PBMCs following the PD-1 blockade treatment is more than 0.9 times, preferably more than 1.0 times, most preferably more than 1.1 times the expression level prior to the PD-1 blockade treatment; the expression level of EGR2 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is more than 0.9 times, preferably more than 1.0 times, most preferably more than 1.1 times the expression level prior to the PD-1 blockade treatment; the expression level of EGR1 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is more than 0.8 times, preferably more than 0.9 times, most preferably more than 1.0 times the expression level prior to the PD-1 blockade treatment; the expression level of GADD45G on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the expression level prior to the PD-1 blockade treatment; the expression level of CKS2 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the expression level prior to the PD-1 blockade treatment; the expression level of CDKN1C on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the expression level prior to the PD-1 blockade treatment; the expression level of IER5 on CD8 +  T cells in the PBMCs following the PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the expression level prior to the PD-1 blockade treatment; the number or frequency of Tregs in CD4 +  T cells in PBMCs following PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the number or frequency of Tregs in CD4 +  T cells in PBMCs prior to the PD-1 blockade treatment; the number or frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells in PBMCs following the PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the number or frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells in PBMCs prior to the PD-1 blockade treatment; the number or frequency of CTLA-4 + CD8 +  cells in CD8 +  T cells in PBMCs following the PD-1 blockade treatment is more than 1.0 times, preferably more than 1.1 times, most preferably more than 1.2 times the number or frequency of CTLA-4 + CD8 +  cells in CD8 +  T cells in PBMCs prior to the PD-1 blockade treatment; or a combination. The subject would not expect a clinical benefit from PD-1 blockade treatment at these levels of expression. 
     In some forms of the method, the expression level of PTEN on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of GATAD1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NKTR on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CD276 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NSUN6 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CCAR1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SMYD2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of FBXO9 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NCRNA00153 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NSMAF on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of P2RY4 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of RCOR2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NUB1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of FCRL2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NUCKS1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SHC2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SCD5 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of USP9Y on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of HSPD1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CISH on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of KLF12 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NCF4 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SFRS7 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CDC14A on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of MAP2K5 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of ILl RA on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of MLL on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of HSPBAP1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of MAP4K4 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of HELLS on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of C1QTNF3 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of UBE2D1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of FAIM3 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of IFI44 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of H2BFM on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of GATAD1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of CASP8 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is positively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of SOCS1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of PDCD6 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; the expression level of NY-ESO-1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject; and the expression level of MART-1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment is negatively associated with the expected clinical benefit of PD-1 blockade treatment of the subject. 
     In some forms of the method, the expression prior the PD-1 blockade treatment is compared to a reference level of expression and an expression level of PTEN on CD8 +  T cells in the PBMCs prior to PD-1 blockade treatment greater than the reference level of PTEN indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of GATAD1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of GATAD1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of NKTR on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of NKTR indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of CD276 on CD8 |  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of CD276 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of NSUN6 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of NSUN6 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of CCAR1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of CCAR1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of SMYD2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of SMYD2 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of FBXO9 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of FBXO9 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of NCRNA00153 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of NCRNA00153 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of NSMAF on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of NSMAF indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of P2RY4 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of P2RY4 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of RCOR2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of RCOR2 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of NUB1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of NUB1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of FCRL2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of FCRL2 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of NUCKS1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of NUCKS1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of SHC2 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of SHC2 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of SCD5 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of SCD5 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of USP9Y on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of USP9Y indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of HSPD1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of HSPD1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of CISH on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of CISH indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of KLF12 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of KLF12 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of NCF4 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of NCF4 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of SFRS7 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of SFRS7 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of CDC14A on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of CDC14A indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of MAP2K5 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of MAP2K5 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of IL11RA on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of IL11RA indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of MLL on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of MLL indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of HSPBAP1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of HSPBAP1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of MAP4K4 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of MAP4K4 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of HELLS on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of HELLS indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of C1QTNF3 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of C1QTNF3 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of UBE2D1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of UBE2D1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of FAIM3 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of FAIM3 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of IFI44 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of IFI44 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of H2BFM on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of H2BFM indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of GATAD1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of GATAD1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of CASP8 on CD8 +  T cells in the PBMCs prior to PD-1 blockade greater than the reference level of CASP8 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; an expression level of SOCS1 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of SOCS1 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment; and an expression level of PDCD6 on CD8 +  T cells in the PBMCs prior to PD-1 blockade lower than the reference level of PDCD6 indicates that the subject can expect a clinical benefit from PD-1 blockade treatment. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIGS. 1A, 1B, and 1C  are Kaplan-Meier relapse-free survival curves showing the proportion of patients with high baseline EOMES + /CD8 +  ( FIG. 1A , dashed line), low EOMES + /CD8 +  ( FIG. 1A , solid line), high baseline Ki67 + EOMES + /CD8 +  ( FIG. 1B , dashed line), low Ki67 + EOMES + /CD8 +  ( FIG. 1B , solid line), high level of CCR7 on CD4 +  cells ( FIG. 1C , dashed line), or low level of CCR7 on CD4 +  cells ( FIG. 1C , solid line) as a function of relapse-free survival (months). 
         FIG. 2  is a graph of the percent of Ki67 + EOMES + /CD8 +  cells in CD8 |  PBMCs for normal, relapsed, and NED subjects (subjects formerly diagnosed with cancer but now showing no evidence of disease). p-values in the graph are from Wilcoxon rank-sum test. Overall p-value=0.038 for comparing the three groups from Kruskal-Wallis test. 
         FIG. 3  is a Venn diagram of gene profiling impacted differentially by PD-1 blockade according to dose. 
         FIG. 4  is a principle component analysis (PCA) of pre and post treatment gene expression of CD8 +  T cells for separate clinical responders and non responders to PD-1 blockade treatment. 
         FIG. 5  is a heat map of baseline gene expression associated with clinical response by PD-1 blockade. 
         FIG. 6  is a heat map of post-treatment gene expression associated with clinical response by PD-1 blockade. 
         FIGS. 7A, 7B, 7C, and 7D  are graphs of marker differences in responders and nonresponders to treatment with BMS-936558. There were a total 25 patients with 12 weeks measurement after BMS-936558 treatment; 13 responders and 12 non-responders.  FIG. 7A  is a graph of the percent of Tregs in CD4 +  T cells measured in responders and nonresponders pre and post treatment with BMS-936558.  FIG. 7B  is a graph of the percent change in Tregs for responders (Yes) and nonresponders (No). Tregs (phenotype as FoxP3+CD127LowCD25+CD4+) decreased in responders and increased in non-responders significantly. The change between two groups is significant, too.  FIG. 7C  is a graph of the percent change in CD4 | /CLTA-4 |  T cells for responders (Yes) and nonresponders (No).  FIG. 7D  is a graph of the percent change in CD8 + /CLTA-4 +  T cells for responders (Yes) and nonresponders (No). CTLA-4+ in CD4 and CD8+ has significant change between responders and non-responders. 
         FIGS. 8A, 8B, 8C, and 8D  are graphs of marker differences in responders and nonresponders to treatment with BMS-936558. 33 patients were measured with baseline of the tetramers; 25 with 12 weeks measurement; 13 responders and 12 non-responders. 8 patients only have baseline who are non-responders.  FIG. 8A  is a graph of the percent of NY-ESO-1 Tetramer | CD8 |  T cells measured in responders and nonresponders before treatment with BMS-936558.  FIG. 8B  is a graph of the percent of MART-1 Tetramer +  CD8 +  T cells measured in responders and nonresponders before treatment with BMS-936558. Both NY-ESO-1 and MART-1 specific CD8 +  T cells had higher baseline. Level in non-responders was compared to responders.  FIG. 8C  is a graph of the percent of NY-ESO-1 Tetramer +  CD8 +  T cells measured in responders and nonresponders after treatment with BMS-936558.  FIG. 8D  is a graph of the percent of MART-1 Tetramer +  CD8 +  T cells measured in responders and nonresponders after treatment with BMS-936558. Responders had significant increase in both NY-ESO-1 and MART-1 specific CD8 +  T cells at 12 weeks after BMS-936558 treatment. 
         FIGS. 9A and 9B  are Box plots of CD71 baseline expression in irAE group and non-irAE group of the patients. The line inside the rectangle indicated the median of the baseline CD71 expression distribution. The upper and lower boundaries of the rectangle indicated the upper quartile and the lower quartile, respectively. Two lines (the whiskers) are drawn from the rectangle to the extreme values (highest and lowest expression level of baseline CD71 expression in CD4+ T cells). 
         FIG. 10  is a graph of Kaplan-Meier curves for association of baseline biomarkers (percentage of CD8 +  T cells that are CD109 + ) with relapse free survival. 
         FIG. 11  is a list of identified baseline biomarkers in CD4+ T cells associated with outcome (Relapse vs. NED). 
         FIG. 12  is a list of identified baseline biomarkers in CD8+ T cells associated with outcome (Relapse vs. NED). 
         FIG. 13  is a list of identified baseline biomarkers in CD4+ T cells associated with the induction of irAE (Yes vs. No). 
         FIG. 14  is a list of identified baseline biomarkers in CD8 +  T cells associated with the induction of irAE (Yes vs. No). 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     I. Definitions 
     The term “individual,” “host,” “subject,” and “patient” are used interchangeably to refer to any individual who is the target of administration or treatment. The subject can be a vertebrate, for example, a mammal. Thus, the subject can be a human or veterinary patient. 
     The term “treatment” refers to the medical management of a patient with the intent to cure, ameliorate, stabilize, or prevent a disease, pathological condition, or disorder. This term includes active treatment, that is, treatment directed specifically toward the improvement of a disease, pathological condition, or disorder, and also includes causal treatment, that is, treatment directed toward removal of the cause of the associated disease, pathological condition, or disorder. In addition, this term includes palliative treatment, that is, treatment designed for the relief of symptoms rather than the curing of the disease, pathological condition, or disorder; preventative treatment, that is, treatment directed to minimizing or partially or completely inhibiting the development of the associated disease, pathological condition, or disorder, and supportive treatment, that is, treatment employed to supplement another specific therapy directed toward the improvement of the associated disease, pathological condition, or disorder. 
     The term “cancer” or “malignant neoplasm” refers to a cell that displays uncontrolled growth, invasion upon adjacent tissues, and often metastasis to other locations of the body. 
     The term “metastasis” refers to the spread of malignant tumor cells from one organ or part to another non-adjacent organ or part. Cancer cells can “break away,” “leak,” or “spill” from a primary tumor, enter lymphatic and blood vessels, circulate through the bloodstream, and settle down to grow within normal tissues elsewhere in the body. When tumor cells metastasize, the new tumor is called a secondary or metastatic cancer or tumor. 
     The term “biomarker” as used herein refers to a peptide, protein, or nucleic acid in or on a cell of a subject whose presence and/or level is indicative of a biological process, pathogenic process, or pharmacologic response to therapeutic intervention. In preferred embodiments, detection of the biomarker may be used to predict therapeutic outcome. 
     The term “relapse” as used herein refers to the return of a cancer after a period of improvement, e.g., remission. 
     The term “immune related adverse event” or “irAE” refers to side effects from CTLA-4 blockade therapy that are inflammatory in nature, including rash, colitis, and hepatitis. 
     The term “frequency” as used herein and in the context of the disclosed methods refers to the percentage of PBMCs expressing a particular biomarker or combination of biomarkers at threshold levels. These levels may be determined empirically using standards and controls. 
     The term “expression level” as used herein refers to quantitative or relative amounts of biomarker in or on a cell that is detected by an assay. 
     The level of expression measured for the disclosed biomarkers can be compared to any suitable control or standard level of expression. For example, a normal level (such as the level of the biomarker in a subject or subjects without cancer), a control level (such as the level of the biomarker in a subject or subjects that do not respond and/or do not get clinical benefit from PD-1 blockade treatment), etc. can be used as a comparison to measured levels of expression of the biomarkers. Such levels used for comparison to measured expression levels of the biomarkers can be referred to as reference levels. 
     The term “response,” in the context of CTLA-4 and PD-1 blockade treatment, refers to a positive clinical response to the treatment. Related to this, the term “responder,” in the context of CTLA-4 and PD-1 blockade treatment, refers to a subject that exhibits a response to the treatment. Similarly, the term “non-response,” in the context of CTLA-4 and PD-1 blockade treatment, refers to a lack of clinical response or a negative clinical response to the treatment; and the term “non-responder,” in the context of CTLA-4 and PD-1 blockade treatment, refers to a subject that exhibits a non-response to the treatment. 
     II. Biomarkers 
     A. CD8 
     In some embodiments, the biomarker is cluster of differentiation 8 (CD8). CD8 is a transmembrane glycoprotein that serves as a co-receptor for the T cell receptor (TCR). Like the TCR, CD8 binds to a major histocompatibility complex (MHC) molecule, but is specific for the class I MHC protein. There are two isoforms of the protein, alpha and beta, each encoded by a different gene. In humans, both genes are located on chromosome 2 in position 2p12. The CD8 co-receptor is predominantly expressed on the surface of cytotoxic T cells, but can also be found on natural killer cells, cortical thymocytes, and dendritic cells. It is expressed in T cell lymphoblastic lymphoma and hypo-pigmented mycosis fungoides, but is frequently lost in other T-cell neoplasms. 
     Antibodies that specifically bind human CD8 are commercially available and include clone 3B5 (Life Technologies), clone SK1 (Stemcell Technologies), clone SP16 (Spring Biosciences), and OKT8 (eBioscience). 
     B. CD4 
     Cluster of differentiation 4 (CD4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. CD4 is a co-receptor that assists the T cell receptor (TCR) with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR by recruiting an enzyme, known as the tyrosine kinase lck, which is essential for activating many molecules involved in the signaling cascade of an activated T cell. CD4 also interacts directly with MHC class II molecules on the surface of the antigen-presenting cell using its extracellular domain. 
     CD4 continues to be expressed in most neoplasms derived from T helper cells. It is therefore possible to use CD4 immunohistochemistry on tissue biopsy samples to identify most forms of peripheral T cell lymphoma and related malignant conditions. The antigen has also been associated with a number of autoimmune diseases such as vitiligo and type I diabetes mellitus. 
     Antibodies that specifically bind human CD4 are commercially available and include clone L120 (Stemcell Technologies), clone SK3 (Stemcell Technologies), clone RPA-T4 (Imgenex), clone Q4120 (Sigma-Aldrich), and clone SP35 (Spring Biosciences). 
     C. EOMES 
     Eomesodermin homolog (EOMES) is a key transcription factor of cytotoxic lymphocyte lineages. Antibodies that specifically bind human EOMES are commercially available and include clone 1A8 (Abnova), clone 2C7 (Abnova), and clone 3E7 (MPBio). The nucleic acid sequence for human EOMES is set forth in Accession No. NP_005433. 
     D. K167 
     The Ki-67 protein (also known as MKI67) is nuclear protein that is associated with and may be necessary for cellular proliferation. During interphase, Ki-67 can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). 
     Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate, brain and the breast. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis. 
     MIB1 is a commonly used monoclonal antibody that detects the Ki-67 antigen. It is used in clinical applications to determine the Ki-67 labeling index. One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see section Original Ki-67 antibody). 
     E. TGFβR3 
     TGFβ receptors are single pass serine/threonine kinase receptors. They exist in several different isoforms that can be homo- or heterodimeric. The number of characterized ligands in the TGFβ superfamily far exceeds the number of known receptors, suggesting the promiscuity that exists between the ligand and receptor interactions. Three TGF-β receptor types can be distinguished by their structural and functional properties. Receptor types I and II have similar ligand-binding affinities and can be distinguished from each other only by peptide mapping. Both receptor types I and II have a high affinity for TGF-01 and low affinity for TGF-β2. TGF-β receptor type III (TGFβR3) has a high affinity for both TGF-β1 and TGF-32. 
     Antibodies that specifically bind human TGFβR3 are commercially available and include clone ZT001 (Invitrogen), clone D11G10 (Cell Signaling Technologies), and clone 20724 (R&amp;D Systems). The nucleic acid sequence for human TGFβR3 is set forth in Accession No. NM_003243. 
     F. CCR7 
     C—C chemokine receptor type 7 (CCR7) is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells home to lymph nodes, as well as stimulate dendritic cell maturation. CCR7 signaling is also known to play a role in lymph node metastasis. The chemokine (C—C motif) ligand 19 (CCL 19/ELC) has been reported to be a specific ligand of this receptor. 
     Antibodies that specifically bind human CCR7 are commercially available and include clone 150503 (R&amp;D Systems), clone FR11-11E8 (Miltenyi Biotec), and clone 3D12 (eBioscience). The nucleic acid sequence for human CCR7 is set forth in Accession No. NM_001838.2. 
     G. CD71 
     CD71, also known as transferrin receptor protein 1 (TfR1), is a protein encoded by the TFRC gene. CD71 is required for iron delivery from transferrin to cells. 
     Antibodies that specifically bind human CD71 are commercially available and include GR08K-100UG, GR09L-100UG, CBL47 (available from EMD Millipore); and clone 29806 (available from R&amp;D Systems). The nucleic acid sequence for human CD71 is set forth in Accession No. NM_001128148. 
     H. CD109 
     CD109 is a GPI-linked cell surface antigen expressed by CD34+ acute myeloid leukemia cell lines, T-cell lines, activated T lymphoblasts, endothelial cells, and activated platelets (Lin et al.,  Blood  99(5): 1683-91 (2002)). CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-β). 
     Antibodies that specifically bind human ICOS are commercially available and include clone 496920 (available from R&amp;D Systems) and clone NBP1-84393 (available from Novus Biologicals). The nucleic acid sequence for human CD109 is set forth in Accession No. NM_001159587. 
     I. MART-1 
     Melanoma antigen recognized by T-cells 1 (MART-1), also known as melan-A, is encoded by the MLANA gene. A fragment of the protein, usually consisting of the nine amino acids 27 to 35, is bound by MHC class I complexes which present it to T cells of the immune system. These complexes can be found on the surface of melanoma cells. Decameric peptides (26-35) are being investigated as cancer vaccines. 
     Antibodies that specifically bind human MART-1 are commercially available and include clone EP1422Y (available from Novus Biologicals) and clone A103 (available from EMD Millipore). The nucleic acid sequence for human MART-1 is set forth in Accession No. NM_005511. 
     J. NY-ESO-1 
     Cancer/testis antigen 1 (NY-ESO-1) is a protein expressed in testis and various cancers. Antibodies that specifically bind human NY-ESO-1 are commercially available and include clone 3F10 (available from OriGene) and clone 2B6 (available from Novus Biologicals). The nucleic acid sequence for human NY-ESO-1 is set forth in Accession No. NM_001327. 
     III. Methods 
     Methods are disclosed for predicting the efficacy, potential for relapse, and irAE in a subject diagnosed with cancer and undergoing treatment. Methods are also disclosed for predicting the efficacy, potential for relapse, and irAE in a subject diagnosed with cancer and undergoing treatment based on the levels of specific biomarkers measured in the subject. Methods are also disclosed for treating subjects diagnosed with cancer based on the predicted efficacy, potential for relapse, and irAE in the subject. Methods are also disclosed for treating specific subjects selected from subjects diagnosed with cancer based on the predicted efficacy, potential for relapse, and irAE in the subject. Methods are also disclosed for treating subjects diagnosed with cancer based on the levels of specific biomarkers measured in the subject. Methods are also disclosed for treating specific subjects selected from subjects diagnosed with cancer based on the levels of specific biomarkers measured in the subject. 
     Methods are also disclosed for not treating subjects diagnosed with cancer with specific therapies based on the predicted efficacy, potential for relapse, and irAE in the subject. Methods are also disclosed for not treating specific subjects selected from subjects diagnosed with cancer with specific therapies based on the predicted efficacy, potential for relapse, and irAE in the subject. Methods are also disclosed for not treating subjects diagnosed with cancer with specific therapies based on the levels of specific biomarkers measured in the subject. Methods are also disclosed for not treating specific subjects selected from subjects diagnosed with cancer with specific therapies based on the levels of specific biomarkers measured in the subject. 
     Methods are disclosed for predicting the efficacy and clinical benefit in a subject diagnosed with cancer and undergoing treatment. Methods are also disclosed for predicting the efficacy and clinical benefit in a subject diagnosed with cancer and undergoing treatment based on the levels of specific biomarkers measured in the subject. Methods are also disclosed for treating subjects diagnosed with cancer based on the predicted efficacy and clinical benefit in the subject. Methods are also disclosed for treating specific subjects selected from subjects diagnosed with cancer based on the predicted efficacy and clinical benefit in the subject. Methods are also disclosed for treating subjects diagnosed with cancer based on the levels of specific biomarkers measured in the subject. Methods are also disclosed for treating specific subjects selected from subjects diagnosed with cancer based on the levels of specific biomarkers measured in the subject. 
     Methods are also disclosed for not treating subjects diagnosed with cancer with specific therapies based on the predicted efficacy and clinical benefit in the subject. Methods are also disclosed for not treating specific subjects selected from subjects diagnosed with cancer with specific therapies based on the predicted efficacy and clinical benefit in the subject. Methods are also disclosed for not treating subjects diagnosed with cancer with specific therapies based on the levels of specific biomarkers measured in the subject. Methods are also disclosed for not treating specific subjects selected from subjects diagnosed with cancer with specific therapies based on the levels of specific biomarkers measured in the subject. 
     The cancer of the disclosed methods can be any cell in a subject undergoing unregulated growth, invasion, or metastasis. In some aspects, the cancer can be any neoplasm or tumor for which radiotherapy is currently used. Alternatively, the cancer can be a neoplasm or tumor that is not sufficiently sensitive to radiotherapy using standard methods. Thus, the cancer can be a sarcoma, lymphoma, leukemia, carcinoma, blastoma, or germ cell tumor. A representative but non-limiting list of cancers that the disclosed compositions can be used to treat include lymphoma, B cell lymphoma, T cell lymphoma, mycosis fungoides, Hodgkin&#39;s Disease, myeloid leukemia, bladder cancer, brain cancer, nervous system cancer, head and neck cancer, squamous cell carcinoma of head and neck, kidney cancer, lung cancers such as small cell lung cancer and non-small cell lung cancer, neuroblastoma/glioblastoma, ovarian cancer, pancreatic cancer, prostate cancer, skin cancer, liver cancer, melanoma, squamous cell carcinomas of the mouth, throat, larynx, and lung, colon cancer, ccrvical cancer, ccrvical carcinoma, breast cancer, epithelial cancer, renal cancer, genitourinary cancer, pulmonary cancer, esophageal carcinoma, head and neck carcinoma, large bowel cancer, hematopoietic cancers; testicular cancer; colon and rectal cancers, prostatic cancer, and pancreatic cancer. 
     In preferred embodiments, the cancer is a melanoma. In these embodiments, the method may be used to predict efficacy of CTLA-4 blockade therapy, treatment with other neoplastic drugs, or treatment with a combination of CTLA-4 blockade therapy and other neoplastic drugs. 
     In preferred embodiments, the cancer is a melanoma. In these embodiments, the method may be used to predict efficacy and clinical benefit of PD-1 blockade therapy, treatment with other neoplastic drugs, or treatment with a combination of PD-1 blockade therapy and other neoplastic drugs. 
     The disclosed methods involve detecting one or more biomarkers in or on cells in the subject. In preferred embodiments, the one or more biomarkers can be detected in or on PBMCs in the subject, T cells in the subject, T cells in the subject&#39;s PBMCs, CD8 +  T cells in the subject, CD4 +  T cells in the subject, CD8 +  T cells in the subject&#39;s PBMCs, CD4 +  T cells in the subject&#39;s PBMCs, or a combination. In most preferred embodiments the one or more biomarkers can be detected in or on PBMCs in the subject. Biomarkers can be detected using standard methods, such as immunoassays to detect proteins in or on the cells, or nucleic acid detection methods to detect gene expression within the cells. 
     A. Immunoassays 
     In some aspects, one or more biomarkers are detected using an immunoassay. Many types and formats of immunoassays are known and all are suitable for detecting the disclosed biomarkers. Examples of immunoassays are enzyme linked immunosorbent assays (ELISAs), radioimmunoassays (RIA), radioimmune precipitation assays (RIPA), immunobead capture assays, Western blotting, dot blotting, gel-shift assays, flow cytometry, protein arrays, multiplexed bead arrays, magnetic capture, in vivo imaging, fluorescence resonance energy transfer (FRET), and fluorescence rccovcry/localization aftcr photoblcaching (FRAP/FLAP). 
     In general, immunoassays involve contacting a sample suspected of containing a molecule of interest (such as the disclosed biomarkers) with an antibody to the molecule of interest or contacting an antibody to a molecule of interest (such as antibodies to the disclosed biomarkers) with a molecule that can be bound by the antibody, as the case may be, under conditions effective to allow the formation of immunocomplexes. Contacting a sample with the antibody to the molecule of interest or with the molecule that can be bound by an antibody to the molecule of interest under conditions effective and for a period of time sufficient to allow the formation of immune complexes (primary immune complexes) is generally a matter of simply bringing into contact the molecule or antibody and the sample and incubating the mixture for a period of time long enough for the antibodies to form immune complexes with, i.e., to bind to, any molecules (e.g., antigens) present to which the antibodies can bind. In many forms of immunoassay, the sample-antibody composition, such as a tissue section, ELISA plate, dot blot or Western blot, can then be washed to remove any non-specifically bound antibody species, allowing only those antibodies specifically bound within the primary immune complexes to be detected. 
     Immunoassays can include methods for detecting or quantifying the amount of a molecule of interest (such as the disclosed biomarkers or their antibodies) in a sample, which methods generally involve the detection or quantitation of any immune complexes formed during the binding process. In general, the detection of immunocomplex formation is well known in the art and can be achieved through the application of numerous approaches. These methods are generally based upon the detection of a label or marker, such as any radioactive, fluorescent, biological or enzymatic tags or any other known label. 
     As used herein, a label can include a fluorescent dye, a member of a binding pair, such as biotin/streptavidin, a metal (e.g., gold), or an epitope tag that can specifically interact with a molecule that can be detected, such as by producing a colored substrate or fluorescence. Substances suitable for detectably labeling proteins include fluorescent dyes (also known herein as fluorochromes and fluorophores) and enzymes that react with colorometric substrates (e.g., horseradish peroxidase). The use of fluorescent dyes is generally preferred in the practice of the invention as they can be detected at very low amounts. Furthermore, in the case where multiple antigens are reacted with a single array, each antigen can be labeled with a distinct fluorescent compound for simultaneous detection. Labeled spots on the array are detected using a fluorimeter, the presence of a signal indicating an antigen bound to a specific antibody. 
     A modifier unit such as a radionuclide can be incorporated into or attached directly to any of the compounds described herein by halogenation. In another aspect, the radionuclide can be attached to a linking group or bound by a chelating group, which is then attached to the compound directly or by means of a linker. Radiolabeling techniques such as these are routinely used in the radiopharmaceutical industry. 
     Labeling can be either direct or indirect. In direct labeling, the detecting antibody (the antibody for the molecule of interest) or detecting molecule (the molecule that can be bound by an antibody to the molecule of interest) include a label. Detection of the label indicates the presence of the detecting antibody or detecting molecule, which in turn indicates the presence of the molecule of interest or of an antibody to the molecule of interest, respectively. In indirect labeling, an additional molecule or moiety is brought into contact with, or generated at the site of, the immunocomplex. For example, a signal-generating molecule or moiety such as an enzyme can be attached to or associated with the detecting antibody or detecting molecule. The signal-generating molecule can then generate a detectable signal at the site of the immunocomplex. For example, an enzyme, when supplied with suitable substrate, can produce a visible or detectable product at the site of the immunocomplex. ELISAs use this type of indirect labeling. 
     As another example of indirect labeling, an additional molecule (which can be referred to as a binding agent) that can bind to either the molecule of interest or to the antibody (primary antibody) to the molecule of interest, such as a second antibody to the primary antibody, can be contacted with the immunocomplex. The additional molecule can have a label or signal-generating molecule or moiety. The additional molecule can be an antibody, which can thus be termed a secondary antibody. Binding of a secondary antibody to the primary antibody can form a so-called sandwich with the first (or primary) antibody and the molecule of interest. The immune complexes can be contacted with the labeled, secondary antibody under conditions effective and for a period of time sufficient to allow the formation of secondary immune complexes. The secondary immune complexes can then be generally washed to remove any non-specifically bound labeled secondary antibodies, and the remaining label in the secondary immune complexes can then be detected. The additional molecule can also be or include one of a pair of molecules or moieties that can bind to each other, such as the biotin/avadin pair. In this mode, the detecting antibody or detecting molecule should include the other member of the pair. 
     Other modes of indirect labeling include the detection of primary immune complexes by a two-step approach. For example, a molecule (which can be referred to as a first binding agent), such as an antibody, that has binding affinity for the molecule of interest or corresponding antibody can be used to form secondary immune complexes, as described above. After washing, the secondary immune complexes can be contacted with another molecule (which can be referred to as a second binding agent) that has binding affinity for the first binding agent, again under conditions effective and for a period of time sufficient to allow the formation of immune complexes (thus forming tertiary immune complexes). The second binding agent can be linked to a detectable label or signal-generating molecule or moiety, allowing detection of the tertiary immune complexes thus formed. This system can provide for signal amplification. 
     Immunoassays that involve the detection of as substance, such as a protein or an antibody to a specific protein, include label-free assays, protein separation methods (i.e., electrophoresis), solid support capture assays, or in vivo detection. Label-free assays are generally diagnostic means of determining the presence or absence of a specific protein, or an antibody to a specific protein, in a sample. Protein separation methods are additionally useful for evaluating physical properties of the protein, such as size or net charge. Capture assays are generally more useful for quantitatively evaluating the concentration of a specific protein, or antibody to a specific protein, in a sample. Finally, in vivo detection is useful for evaluating the spatial expression patterns of the substance, i.e., where the substance can be found in a subject, tissue or cell. 
     Flow cytometry is a well-known technique for counting and/or otherwise examining microscopic particles, such as cells and the like, by passing a stream of fluid in which the particles are suspended through a detection apparatus. The detection apparatus typically relies on detecting the optical response produced as the particles pass through an illuminated region of the device. In some forms, for example, individual particles pass through an illumination zone, typically at a rate on the order of 1,000 cells per second, and detectors, gated electronically, measure the magnitude of a pulse representing the light scattered by the cells. The pulse magnitudes (or other properties) may then be processed to characterize the cells by a particular parameter of interest. For example, the angular dependence of scattered light may provide information on the nature of the scattering particles. More importantly, the fluorescent properties of the particles (which may be caused by appropriate fluorophores being added to the suspension) may provide desired parametric information. Exemplary flow cytometry systems are disclosed in U.S. Pat. No. 5,760,900 and U.S. Patent Publication No. 2008/0186479, which are hereby incorporated by reference herein in their entirety. Those of skill in the art are aware of other systems and techniques for flow cytometry, any of which can be adapted for use with the disclosed methods. 
     B. Nucleic Acid Detection 
     In some aspects, one or more biomarkers are nucleic acids, such as mRNA. A number of widely used procedures exist for detecting and determining the abundance of a particular mRNA in a total or poly(A) RNA sample. For example, specific mRNAs can be detected using Northern blot analysis, nuclease protection assays (NPA), in situ hybridization, or reverse transcription-polymerase chain reaction (RT-PCR). 
     In theory, each of these techniques can be used to detect specific RNAs and to precisely determine their expression level. In general, Northern analysis is the only method that provides information about transcript size, whereas NPAs are the easiest way to simultaneously examine multiple messages. In situ hybridization is used to localize expression of a particular gene within a tissue or cell type, and RT-PCR is the most sensitive method for detecting and quantitating gene expression. 
     Northern analysis presents several advantages over the other techniques. The most compelling of these is that it is the easiest method for determining transcript size, and for identifying alternatively spliced transcripts and multigene family members. It can also be used to directly compare the relative abundance of a given message between all the samples on a blot. The Northern blotting procedure is straightforward and provides opportunities to evaluate progress at various points (e.g., intactness of the RNA sample and how efficiently it has transferred to the membrane). RNA samples are first separated by size via electrophoresis in an agarose gel under denaturing conditions. The RNA is then transferred to a membrane, crosslinked and hybridized with a labeled probe. Nonisotopic or high specific activity radiolabeled probes can be used including random-primed, nick-translated, or PCR-generated DNA probes, in vitro transcribed RNA probes, and oligonucleotides. Additionally, sequences with only partial homology (e.g., cDNA from a different species or genomic DNA fragments that might contain an exon) may be used as probes. 
     The Nuclease Protection Assay (NPA) (including both ribonuclease protection assays and SI nuclease assays) is an extremely sensitive method for the detection and quantitation of specific mRNAs. The basis of the NPA is solution hybridization of an antisense probe (radiolabeled or nonisotopic) to an RNA sample. After hybridization, single-stranded, unhybridized probe and RNA are degraded by nucleases. The remaining protected fragments are separated on an acrylamide gel. Solution hybridization is typically more efficient than membrane-based hybridization, and it can accommodate up to 100 μg of sample RNA, compared with the 20-30 μg maximum of blot hybridizations. NPAs are also less sensitive to RNA sample degradation than Northern analysis since cleavage is only detected in the region of overlap with the probe (probes are usually about 100-400 bases in length). NPAs are the method of choice for the simultaneous detection of several RNA species. During solution hybridization and subsequent analysis, individual probe/target interactions are completely independent of one another. Thus, several RNA targets and appropriate controls can be assayed simultaneously (up to twelve have been used in the same reaction), provided that the individual probes are of different lengths. NPAs are also commonly used to precisely map mRNA termini and intron/exon junctions. 
     In situ hybridization (ISH) is a powerful and versatile tool for the localization of specific mRNAs in cells or tissues. Unlike Northern analysis and nuclease protection assays, ISH does not require the isolation or electrophoretic separation of RNA. Hybridization of the probe takes place within the cell or tissue. Since cellular structure is maintained throughout the procedure, ISH provides information about the location of mRNA within the tissue sample. The procedure begins by fixing samples in neutral-buffered formalin, and embedding the tissue in paraffin. The samples are then sliced into thin sections and mounted onto microscope slides. (Alternatively, tissue can be sectioned frozen and post-fixed in paraformaldehyde.) After a series of washes to dewax and rehydrate the sections, a Proteinase K digestion is performed to increase probe accessibility, and a labeled probe is then hybridized to the sample sections. Radiolabeled probes are visualized with liquid film dried onto the slides, while nonisotopically labeled probes are conveniently detected with colorimetric or fluorescent reagents. 
     RT-PCR has revolutionized the study of gene expression. It is now theoretically possible to detect the RNA transcript of any gene, regardless of the scarcity of the starting material or relative abundance of the specific mRNA. In RT-PCR, an RNA template is copied into a complementary DNA (cDNA) using a retroviral reverse transcriptase. The cDNA is then amplified exponentially by PCR. As with NPAs, RT-PCR is somewhat tolerant of degraded RNA. As long as the RNA is intact within the region spanned by the primers, the target will be amplified. 
     Relative quantitative RT-PCR involves amplifying an internal control simultaneously with the gene of interest. The internal control is used to normalize the samples. Once normalized, direct comparisons of relative abundance of a specific mRNA can be made across the samples. It is crucial to choose an internal control with a constant level of expression across all experimental samples (i.e., not affected by experimental treatment). Commonly used internal controls (e.g., GAPDH, tactin, cyclophilin) often vary in expression and, therefore, may not be appropriate internal controls. Additionally, most common internal controls are expressed at much higher levels than the mRNA being studied. For relative RT-PCR results to be meaningful, all products of the PCR reaction must be analyzed in the linear range of amplification. This becomes difficult for transcripts of widely different levels of abundance. 
     Competitive RT-PCR is used for absolute quantitation. This technique involves designing, synthesizing, and accurately quantitating a competitor RNA that can be distinguished from the endogenous target by a small difference in size or sequence. Known amounts of the competitor RNA are added to experimental samples and RT-PCR is performed. Signals from the endogenous target are compared with signals from the competitor to determine the amount of target present in the sample. 
     C. CTLA-4 Blockade Therapies 
     CTLA-4 (cytotoxic T lymphocyte-associated antigen 4) is a molecule on cytotoxic T lymphocytes that is believed to play a critical role in regulating natural immune responses. The absence or presence of CTLA-4 can augment or suppress the immune system&#39;s T-cell response in fighting disease. Antibodies designed to block the activity of CTLA-4 are effective in sustaining an active immune response in its attack on cancer cells. CTLA-4 blockade therapy can, for example, target CTLA-4. The composition or compound used can block the function of the CTLA-4. 
     For example, Ipilimumab is a fully human antibody that binds to CTLA-4. Ipilimumab was approved by the FDA in March 2011 to treat patients with late-stage melanoma that has spread or cannot be removed by surgery. Additionally, ipilimumab is undergoing clinical trials for the treatment of non-small cell lung carcinoma (NSCLC), small cell lung cancer (SCLC) and metastatic hormone-refractory prostate cancer. Tremelimumab is a fully human IgG2 monoclonal antibody that binds to CTLA-4. 
     D. Non CTLA-4 Anti-Neoplastic Therapies 
     Numerous anti-cancer (antineoplastic) drugs are available for treating subjects with cancer. In some embodiments, the disclosed biomarkers may also be used to predict efficacy, relapse, and irAE in a subject treated with one or more of these anti-neoplastic drugs, alone or in combination with CTLA-4 blockade therapy. 
     E. PD-1 Blockade Therapies 
     Programmed Death-1 (PD-1) is a negative regulator of T cell activation and proliferation that mediates suppressive action by binding to its ligands PD-L1 and PD-L2. T cell exhaustion is a state of T cell dysfunction that arises during many chronic infections and cancer. Over the last few years, it has been established that PD-1 plays an active and reversible role in T cell exhaustion. Under physiological conditions, PD-1 is induced after T cell activation and serves as an inhibitory feedback to dampen the TCR signaling cascade and prevent excessive T cell activation. PD-1 plays also an important role in the tolerance to self antigens. Anti-PD-1 antibodies, such as BMS-936558 and BMS-936559, have been identified as potential cancer therapeutic. BMS-936558 is an antibody against PD-1 and is currently in clinical trials. PD-1 blockade therapy can target either or both the PD-1 receptor or the ligand of the PD-1 receptor. The composition or compound used can block the function of the PD-1 receptor. 
     F. Non PD-1 Anti-Neoplastic Therapies 
     Numerous anti-cancer (antineoplastic) drugs are available for treating subjects with cancer. In some embodiments, the disclosed biomarkers may also be used to predict efficacy and clinical benefit in a subject treated with one or more of these anti-neoplastic drugs, alone or in combination with PD-1 blockade therapy. 
     Antineoplastic drugs include Acivicin, Aclarubicin, Acodazole Hydrochloride, AcrQnine, Adozelesin, Aldesleukin, Altretamine, Ambomycin, Ametantrone Acetate, Aminoglutethimide, Amsacrine, Anastrozole, Anthramycin, Asparaginase, Asperlin, Azacitidine, Azetepa, Azotomycin, Batimastat, Benzodepa, Bicalutamide, Bisantrene Hydrochloride, Bisnafide Dimesylate, Bizelesin, Bleomycin Sulfate, Brequinar Sodium, Bropirimine, Busulfan, Cactinomycin, Calusterone, Caraccmide, Carbctimer, Carboplatin, Carmustine, Carubicin Hydrochloride, Carzelesin, Cedefingol, Chlorambucil, Cirolemycin, Cisplatin, Cladribine, Crisnatol Mesylate, Cyclophosphamide, Cytarabine, Dacarbazine, Dactinomycin, Daunorubicin Hydrochloride, Decitabine, Dexormaplatin, Dezaguanine, Dezaguanine Mesylate, Diaziquone, Docetaxel, Doxorubicin, Doxorubicin Hydrochloride, Droloxifene, Droloxifene Citrate, Dromostanolone Propionate, Duazomycin, Edatrexate, Eflomithine Hydrochloride, Elsamitrucin, Enloplatin, Enpromate, Epipropidine, Epirubicin Hydrochloride, Erbulozole, Esorubicin Hydrochloride, Estramustine, Estramustine Phosphate Sodium, Etanidazole, Ethiodized Oil I 131, Etoposide, Etoposide Phosphate, Etoprine, Fadrozole Hydrochloride, Fazarabine, Fenretinide, Floxuridine, Fludarabine Phosphate, Fluorouracil, Flurocitabine, Fosquidone, Fostriecin Sodium, Gemcitabine, Gemcitabine Hydrochloride, Gold Au 198, Hydroxyurea, Idarubicin Hydrochloride, Ifosfamide, Ilmofosine, Interferon Alfa-2a, Interferon Alfa-2b, Interferon Alfa-n1, Interferon Alfa-n3, Interferon Beta-Ia, Interferon Gamma-Ib, Iproplatin, Irinotecan Hydrochloride, Lanreotide Acetate, Letrozole, Leuprolide Acetate, Liarozole Hydrochloride, Lometrexol Sodium, Lomustine, Losoxantrone Hydrochloride, Masoprocol, Maytansine, Mechlorethamine Hydrochloride, Megestrol Acetate, Melengestrol Acetate, Melphalan, Menogaril, Mercaptopurine, Methotrexate, Methotrexate Sodium, Metoprine, Meturedepa, Mitindomide, Mitocarcin, Mitocromin, Mitogillin, Mitomalcin, Mitomycin, Mitosper, Mitotane, Mitoxantrone Hydrochloride, Mycophenolic Acid, Nocodazole, Nogalamycin, Ormaplatin, Oxisuran, Paclitaxel, Pegaspargase, Peliomycin, Pentamustine, Peplomycin Sulfate, Perfosfamide, Pipobroman, Piposulfan, Piroxantrone Hydrochloride, Plicamycin, Plomestane, Porfimer Sodium, Porfiromycin, Prednimustine, Procarbazine Hydrochloride, Puromycin, Puromycin Hydrochloride, Pyrazofurin, Riboprine, Rogletimide, Safmgol, Safingol Hydrochloride, Semustine, Simtrazene, Sparfosate Sodium, Sparsomycin, Spirogermanium Hydrochloride, Spiromustine, Spiroplatin, Streptonigrin, Streptozocin, Strontium Chloride Sr 89, Sulofenur, Talisomycin, Taxane, Taxoid, Tecogalan Sodium, Tegafur, Teloxantrone Hydrochloride, Temoporfin, Teniposide, Teroxirone, Testolactone, Thiamiprine, Thioguanine, Thiotepa, Tiazofurin, Tirapazamine, Topotecan Hydrochloride, Torcmifene Citrate, Trestolone Acetate, Triciribine Phosphate, Trimetrexate, Trimetrexate Glucuronate, Triptorelin, Tubulozole Hydrochloride, Uracil Mustard, Uredepa, Vapreotide, Verteporfin, Vinblastine Sulfate, Vincristine Sulfate, Vindesine, Vindesine Sulfate, Vinepidine Sulfate, Vinglycinate Sulfate, Vinleurosine Sulfate, Vinorelbine Tartrate, Vinrosidine Sulfate, Vinzolidine Sulfate, Vorozole, Zeniplatin, Zinostatin, Zorubicin Hydrochloride. 
     G. Monitoring Treatment 
     Biomarkers have also been discovered that can be used to monitor subjects following treatment to determine the effectiveness of the treatment and/or the prognosis for the subject. For example, certain biomarkers are associated with patients less likely to relapse or an irAE following CTLA-4 blockade therapy. As another example, certain biomarkers are associated with patients more likely to respond to treatment following PD-1 blockade therapy. 
     A decrease of Ki67 + EOMES + CD4 +  cells in CD4 +  T cells, a decrease in expression of CCR7 on CD8 +  T cells, a decrease in the frequency of CCR7 + CD8 + , cells in CD8 +  T cells, an increase in expression of TGFβR3 on CD8 +  T cells, an increase in expression of EOMES in CD8 +  cells, an increase in frequency of EOMES + CD8 +  cells in CD8 +  T cells, an increase in the frequency of GranzymeB + EOMES + CD8 +  cells in CD8 +  T cells, an increase in the frequency of Perforin + EOMES + CD8 +  cells in CD8 +  T cells, an increase in the frequency of CTLA-4 + CD4 +  cells in CD4 +  T cells, or a combination indicates a higher risk of relapse (that is, a poorer prognosis or an indication that therapy is not successful) in subjects with cancer that have been treated with CTLA-4 blockade therapy. A decrease of Ki67 + CD8 +  cells in CD8 +  T cells, a decrease of CD71 + CD8 +  cells in CD8 +  T cells, a decrease of ICOS + CD8 +  cells in CD8 +  T cells, a decrease of PD-1 + CD8 +  cells in CD8 +  T cells, or a combination indicates a higher risk of developing irAE (that is, a poorer prognosis or an indication that therapy is not successful) in subjects with cancer that have been treated with CTLA-4 blockade therapy. Thus, these biomarkers can be used to assess the progress and effectiveness of CTLA-4 blockade treatment. 
     An increase in NY-ESO-1 + CD8 +  cells in CD8 +  T cells or an increase in MART-1 | CD8 |  cells in CD8+ T cells, or a combination indicates a lower risk of relapse (that is, a better prognosis or an indication that therapy is successful) in subjects with cancer that have been treated with PD-1 blockade therapy (see  FIGS. 8C and 8D ). 
     The disclosed methods include the determination, identification, indication, correlation, diagnosis, prognosis, etc. (which can be referred to collectively as “identifications”) of subjects, diseases, conditions, states, etc. based on measurements, detections, comparisons, analyses, assays, screenings, etc. For example, subjects diagnosed with cancer can be identified for specific treatments (or avoidance of specific treatments) based on the levels of specific biomarkers measured in the subject Such identifications are useful for many reasons. For example, and in particular, such identifications allow specific actions to be taken based on, and relevant to, the particular identification made. For example, diagnosis of a particular disease or condition in particular subjects (and the lack of diagnosis of that disease or condition in other subjects) has the very useful effect of identifying subjects that would benefit from treatment, actions, behaviors, etc. based on the diagnosis. For example, treatment for a particular disease or condition in subjects identified is significantly different from treatment of all subjects without making such an identification (or without regard to the identification). Subjects needing or that could benefit from the treatment will receive it and subjects that do not need or would not benefit from the treatment will not receive it. 
     Accordingly, also disclosed herein are methods comprising taking particular actions following and based on the disclosed identifications. For example, disclosed are methods comprising creating a record of an identification (in physical—such as paper, electronic, or other-form, for example). Thus, for example, creating a record of an identification based on the disclosed methods differs physically and tangibly from merely performing a measurement, detection, comparison, analysis, assay, screen, etc. Such a record is particularly substantial and significant in that it allows the identification to be fixed in a tangible form that can be, for example, communicated to others (such as those who could treat, monitor, follow-up, advise, etc. the subject based on the identification); retained for later use or review; used as data to assess sets of subjects, treatment efficacy, accuracy of identifications based on different measurements, detections, comparisons, analyses, assays, screenings, etc., and the like. For example, such uses of records of identifications can be made, for example, by the same individual or entity as, by a different individual or entity than, or a combination of the same individual or entity as and a different individual or entity than, the individual or entity that made the record of the identification. The disclosed methods of creating a record can be combined with any one or more other methods disclosed herein, and in particular, with any one or more steps of the disclosed methods of identification. 
     As another example, disclosed are methods comprising making one or more further identifications based on one or more other identifications. For example, particular treatments, monitorings, follow-ups, advice, etc. can be identified based on the other identification. For example, identification of a subject as having a disease or condition with a high level of a particular component or characteristic can be further identified as a subject that could or should be treated with a therapy based on or directed to the high level component or characteristic. A record of such further identifications can be created (as described above, for example) and can be used in any suitable way. Such further identifications can be based, for example, directly on the other identifications, a record of such other identifications, or a combination. Such further identifications can be made, for example, by the same individual or entity as, by a different individual or entity than, or a combination of the same individual or entity as and a different individual or entity than, the individual or entity that made the other identifications. The disclosed methods of making a further identification can be combined with any one or more other methods disclosed herein, and in particular, with any one or more steps of the disclosed methods of identification. 
     As another example, disclosed are methods comprising treating, monitoring, following-up with, advising, etc., a subject identified in any of the disclosed methods. Also disclosed are methods comprising treating, monitoring, following-up with, advising, etc., a subject for which a record of an identification from any of the disclosed methods has been made. For example, particular treatments, monitorings, follow-ups, advice, etc. can be used based on an identification and/or based on a record of an identification. For example, a subject identified as having a disease or condition with a high level of a particular component or characteristic (and/or a subject for which a record has been made of such an identification) can be treated with a therapy based on or directed to the high level component or characteristic. Such treatments, monitorings, follow-ups, advice, etc. can be based, for example, directly on identifications, a record of such identifications, or a combination. Such treatments, monitorings, follow-ups, advice, etc. can be performed, for example, by the same individual or entity as, by a different individual or entity than, or a combination of the same individual or entity as and a different individual or entity than, the individual or entity that made the identifications and/or record of the identifications. The disclosed methods of treating, monitoring, following-up with, advising, etc. can be combined with any one or more other methods disclosed herein, and in particular, with any one or more steps of the disclosed methods of identification. 
     EXAMPLES 
     Example 1: Biomarkers on Melanoma Patient T Cells Associated with Ipilimumab Therapy 
     Materials and Methods 
     Patients 
     Between June 2004 and December 2008, 75 patients (39 at the University of Southern California, Norris Cancer Center and 36 at the Moffitt Cancer Center) with resected stage IIIC/IV melanoma received ipilimumab with or without a peptide vaccine. The demographic and clinic outcomes of the patients for this study were shown in Table 1, which have been reported previously (Sarnaik A A, et al.  Clin Cancer Res.  17(4):896-906 (2010)) with updated follow-up: three patients died (R DOD); one patient relapsed and was resected with no evidence of disease (R NED) instead of no evidence of disease (NED). Toxicity was assessed by National Cancer Institute Common Terminology Criteria for Adverse Events, version 3.0. The protocol was approved by the University of Southern California/Los Angeles County and University of South Florida Institutional Review Boards, and all patients provided written informed consent. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Demographic data for the total 55 patients 
               
            
           
           
               
               
               
               
            
               
                   
                 Variable 
                 Level 
                 n (%) 
               
               
                   
               
               
                   
                 dosage 
                 10 mg/kg 
                 40 (72.7) 
               
               
                   
                   
                  3 mg/kg 
                 15 (27.3) 
               
               
                   
                 gender 
                 F 
                 21 (38.2) 
               
               
                   
                   
                 M 
                 34 (61.8) 
               
               
                   
                 stage 
                 III 
                 24 (43.6) 
               
               
                   
                   
                 IV 
                 31 (56.4) 
               
               
                   
                 HLA_A2 
                 A2+ 
                 34 (61.8) 
               
               
                   
                   
                 A2− 
                 21 (38.2) 
               
               
                   
                 irAE 
                 N 
                 31 (56.4) 
               
               
                   
                   
                 Y 
                 24 (43.6) 
               
               
                   
                 Outcome 
                 NED 
                 35 (63.6) 
               
               
                   
                   
                 Relapse 
                 20 (36.4) 
               
               
                   
               
            
           
         
       
     
     PBMC Collection, Preparation and T Cells Purification 
     Apheresis with exchange of 5 to 7 liters was performed within 1 week before and 6 months after the initiation of therapy, after four doses of ipilimumab. Heparinized blood samples were collected after 2 doses of ipilimumab, 3 months after the initiation of therapy. PBMC were isolated from pre-, 3-month and 6-month post-ipilimumab treatment specimens by Lymphoprep (Greiner Bio-One, Longwood, Fla.) density gradient centrifugation. PBMC were counted, then frozen in 90% heat inactivated human AB serum (HS, Omega Scientific, Tarzana, Calif.) and 10% dimethyl sulfoxide (DMSO) (Sigma, St Louis, Mo.) and stored in secured liquid nitrogen freezers at −168° C. until use. 
     Cryopreserved pre- and 6-month post-treatment PBMC of each patient were thawed immediately into pre-warmed AIM-V media (Invitrogen Corporation, Grand Island, N.Y.) supplemented with 5% HS. For microarray analysis, PBMCs were sorted as follows: cells were stained with CD3-FITC (BD Bioscience, San Diego, Calif.), CD4-PE (BD Bioscience), CD8-APC (BD Bioscience) and CD19-PECy7 (BD Bioscience) for 30 min at 4° C. After extensive wash, PBMCs were stained with DAPI nucleic acid dye (Invitrogen) to exclude dead and dying cells. After washes with staining buffer (PBS+0.5% HS), PBMC were re-suspended in staining buffer for flow cytometry sorting. CD3 + CD4 + CD8 − , CD3 + CD8 + CD4 −  T cells and CD3 −  CD19 +  B cell populations were collected by Aria (BD Bioscience, San Jose). The purity of the sorted specific cell populations were consistently ≥99%. 
     Microarray Analysis 
     The sorted cells were pelleted and re-suspended in a RLT lysis buffer (Qiagen Science, Valencia, Calif.), and delivered to the Microarray Core (Moffitt Cancer Center, Tampa, Fla.) for expression array analysis. RNA from the sorted cell populations were DNase-treated and extracted using the Qiagen RNeasy mini-prep according to the manufacturer&#39;s protocol (Qiagen). The Nugen Message Amp Premier kit was used to amplify 100 nanograms of total RNA (Nugen Technologies, San Carlos, Calif.). Briefly, the extracted poly-(A) RNA was specifically converted to cDNA, amplified and labeled with biotin following the procedure initially described by Van Gelder et al. (Van Gelder R N, et al.  Proc Natl Acad Sci USA.  87:1663-7 (1990)). Biotin-labeled cDNA was hybridized onto Affymetrix U133 Plus 2.0 microarrays. The hybridization, staining, and scanning of the chips followed the procedures outlined in the Affymetrix technical manual (Liu W M, et al.  Bioinformatics.  18:1593-9 (2002)). Scanned output files were visually inspected for hybridization artifacts and then analyzed by using Affymetrix GeneChip Operating Software (GCOS). Arrays were scaled to an average intensity of 500 and analyzed independently. The GCOS software used a statistical algorithm to determine the signal intensity of a transcript from the behavior of 11 different oligonucleotide probes designed to detect the same gene. Probe sets that yielded a p-value less than 0.002 were identified as changed. Alternatively, signal intensity was calculated by the robust multi-array analysis method (RMA) developed by Irizarry et al (Irizarry R A, et al.  Nucleic Acids Res.  31:e15 (2003)). Gene changes were then selected using the fold-change cut-off available in RMA or using the Significance Analysis of Microarrays (SAM) technique of Tusher et al. (Tusher V G, et al.  Proc Natl Acad Sci USA.  98:5116-21 (2001)). 
     Surface and Intracellular Staining 
     For cell surface staining, 1-2×10 6  PBMC in 100 μl PBS were stained with the Aqua-live/dead fixable dye (Invitrogen) at 4° C. for 30 min. After washing, PBMC were stained with fluorochrome-conjugated antibodies against cell surface markers prepared as a master-mix-solution for 30 min at 4° C. The antibodies for surface markers were purchased from BD Bioscience unless otherwise specified: CD3-AlexFluor 700, CD4-PECy7, CD8-PERCPCy5.5, ICOS-PE, CCR7-FITC (R&amp;D Systems, Minneapolis, Minn.), CCR7-PE (R &amp;D Systems), CXCR3-FITC (R &amp; D Systems), CD109-PE, CD45RA-FITC, CD28-APC (eBioscience, San Diego, Calif.), IL-7R-APC (R&amp;D system), CD25-FITC, TGFβR3-PE (R&amp;D Systems) and MIC-A-APC (R&amp;D Systems). The fluorescent minus one and isotype control was included in each experiment in order to appropriately set the gates. A total of 300,000 live single lymphocytes were acquired on a LSR II flow cytometer (BD Bioscience). Analysis gates were set on single live lymphocytes defined by scatter characteristics and Aqua negative staining. First, the lymphocytes were gated by the forward and sideward scatters, then the single cells were gated by forward and sideward width and height. The single live lymphocytes were observed by gating on aqua negative stained single lymphocytes. In the whole study, we observed the biomarkers on CD4 and CD8 +  T cells. 
     For intracellular staining, 2-3×10 6  PBMC in 100 μl staining buffer were treated as above, then fixed with a freshly prepared Fixation/Permeabilization working solution (eBioscience) at 4° C. for 30 min. After wash with permeabilization buffer (eBioscience), the cells were stained with intracellular markers: Ki67-FITC (Abcam, Cambridge, Mass.), EOMESodermin (EOMES)-Alex Fluor 647 (eBioscience), granzyme B-FITC, perforin-PE, FoxP3-APC (eBioscience), GATA 3-Alex Fluor 647 (eBioscience), cleaved caspase III-PE for 30 min at 4° C. Cells were then washed in the permeabilization buffer and resuspended in staining buffer for flow cytometry acquisition. 
     For the standardization of flow cytometry assays and the consistency of daily performance, a normal PBMC sample was stained and run on flow cytometry in parallel with the subject samples and Spherotech rainbow fluorescent particles (Spherotech, Tnc, Lake Forest, TL) were run before running samples for alignment of the optical system of the flow cytometer in each channel. 
     Flow Cytometry Analysis 
     Flow cytometry data were analyzed using Flowjo software (Version 9.0.2, Tree Star, Inc., Asland, Oreg.). The mean fluorescence intensity (MFI) and frequency (%) of each marker was measured for CD4 +  and CD8 +  T cells. 
     Statistical Analysis 
     Descriptive summary statistics, that is, frequency and percentage for discrete variables and mean (standard deviation: STD), interquartiles and median (range) for continuous variables, were reported. Both point estimates and their confidence intervals (CIs) were provided for parameters of interest, e.g., mean change or odds ratio (OR). Wilcoxon signed-rank test was used to test whether changes in immunological biomarker parameters between pre-, 3-month and 6-month post-ipilimumab were statistically significant. Both absolute change and relative change, i.e., post-pre and (post-pre)/pre*100, were analyzed to account for a potentially better normalization under the log scale for the immunological biomarker parameters under investigation. Confidence intervals were set at the 95% level. Univariable logistic regression models were employed to explore the effect of absolute change as well as relative change of each biomarker on disease outcome (NED vs. relapsed) and dose limiting irAE (Yes vs. No). The CI for OR based on the univariable logistic regression model results were calculated for an increment that equals one half of the interquartile range of the corresponding change variable for the biomarker. A p-value of ≤0.05 was considered statistically significant. 
     Results 
     Changes in Gene Expression Patterns of CD4 +  and CD8 +  T Cells Induced by Ipilimumab 
     To assess the effect of ipilimumab on global gene expression in CD4 +  and CD8 +  T cells in vivo, microarray analysis was conducted on T cells purified by flow cytometry from PBMCs collected from 12 resected melanoma patients pre- and 6-month post-ipilimumab treatment (Table 6a). The genes whose expression were most significantly altered in CD4 +  and CD8 +  T cells by ipilimumab treatment are listed in Tables 2a and 2b, categorized by T cell biology and function. The genes were grouped into cell cycle, cytokine-related, chemokine-related, T cell activation and proliferation signal-related, survival and apoptotic signal-related, kinase-related and heat shock protein family-related genes. It is worthy to note that the genes most impacted by ipilimumab were cell cycle related in both CD4 +  and CD8 +  T cells. Ipilimumab appeared to release the arrested cell cycle in CD4 +  and CD8 +  T cells as evidenced by significantly up-regulated CDC2 (Tables 2a and 2b), and other cell cycle related genes. Fold change was filtered as an increase ≥1.2 or a decrease ≤0.8-fold, and was statistically significantly different with p≤0.03 by paired Student T-test. The reported genes were all consistently changed in ≥66.67% patients&#39; pre and post ipilimumab by Affymetrix present/absent call. 
     
       
         
           
               
             
               
                 TABLE 6a 
               
             
            
               
                   
               
               
                 Patient Demographics for the Microarray cohort (n = 12) 
               
            
           
           
               
               
               
               
            
               
                   
                 Variable 
                 Level 
                 n (%) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 gender 
                 F 
                 4 
                 (33.3) 
               
               
                   
                   
                 M 
                 8 
                 (66.7) 
               
               
                   
                 stage 
                 III 
                 4 
                 (33.3) 
               
               
                   
                   
                 IV 
                 8 
                 (66.7) 
               
               
                   
                 HLA A2 
                 A2+ 
                 7 
                 (58.3) 
               
               
                   
                   
                 A2− 
                 5 
                 (41.7) 
               
               
                   
                 dosage 
                 10 mg/kg 
                 12 
                 (100) 
               
               
                   
                   
                  3 mg/kg 
                 0 
                 (0) 
               
               
                   
                 irAE 
                 N 
                 7 
                 (58.3) 
               
               
                   
                   
                 Y 
                 5 
                 (41.7) 
               
               
                   
                 Outcome 
                 NED 
                 12 
                 (100) 
               
               
                   
                   
                 Relapse 
                 0 
                 (0) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2a 
               
             
            
               
                   
               
               
                 genes impacted by ipilimumab in CD4+ T cells 
               
            
           
           
               
               
               
               
               
               
            
               
                 Gene symbol 
                 Fold 
                   
                   
                   
                   
               
               
                 Gene name 
                 change 
                 p-value 
                 FDR 
                 Pre-Mean ± SD 
                 Post-Mean ± SD 
               
               
                   
               
            
           
           
               
            
               
                 Cell cycle-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 CDC2 
                 1.98, 
                 0.0090, 
                 0.0409, 
                  18.61 ± 4.41, 
                  36.92 ± 21.36, 
               
               
                 cell division cycle 
                 1.76, 
                 0.0245, 
                 0.0723, 
                   7.05 ± 1.44, 
                  12.41 ± 6.42, 
               
               
                 2, G1 to S and G2 
                 1.51 
                 0.0189 
                 0.0568 
                  21.16 ± 6.79 
                  36.37 ± 17.62 
               
               
                 to M 
                   
                   
                   
                   
                   
               
               
                 TYMS 
                 2.78, 
                 0.0142, 
                 0.0432, 
                  66.68 ± 59.31, 
                  185.59 ± 185.36, 
               
               
                 thymidylate 
                 2.77 
                 0.0075 
                 0.0408 
                  129.75 ± 58.10 
                  358.99 ± 254.47 
               
               
                 synthetase 
                   
                   
                   
                   
                   
               
               
                 CCNB2 
                 2.08 
                 0.0142 
                 0.0434 
                  28.17 ± 11.75 
                  58.49 ± 45.13 
               
               
                 cyclin B2 
                   
                   
                   
                   
                   
               
               
                 CDK7 
                 1.26 
                 0.0074 
                 0.0408 
                  214.08 ± 75.01 
                  270.13 ± 106.34 
               
               
                 cyclin-dependent 
                   
                   
                   
                   
                   
               
               
                 kinase 7 
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Cytokines-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 PGDS 
                 2.2 
                 0.0024 
                 0.0393 
                  14.20 ± 5.42 
                  31.30 ± 14.57 
               
               
                 prostaglandin D2 
                   
                   
                   
                   
                   
               
               
                 synthase, 
                   
                   
                   
                   
                   
               
               
                 hematopoietic 
                   
                   
                   
                   
                   
               
               
                 TGFBR3 
                 1.51, 
                 0.0048, 
                 0.04044, 
                  489.68 ± 470.87, 
                  740.39 ± 581.83, 
               
               
                 transforming 
                 1.39 
                 0.0111 
                 0.0411 
                  505.71 ± 304.47 
                  701.22 ± 246.45 
               
               
                 growth factor b 
                   
                   
                   
                   
                   
               
               
                 RIII 
                   
                   
                   
                   
                   
               
               
                 IFNg 
                 1.46 
                 0.0255 
                 0.0751 
                  138.35 ± 104.72 
                  201.55 ± 120.78 
               
               
                 interferon, g 
                   
                   
                   
                   
                   
               
               
                 IL12A 
                 1.33 
                 0.001 
                 0.0375 
                  11.81 ± 2.00 
                  15.73 ± 4.01 
               
               
                 interleukin 12 A 
                   
                   
                   
                   
                   
               
               
                 IL-7 
                 1.28 
                 0.0169 
                 0.0511 
                  15.43 ± 10.42 
                  19.73 ± 8.89 
               
               
                 interleukin 7 
                   
                   
                   
                   
                   
               
               
                 STAT1 
                 1.28 
                 0.0081 
                 0.0409 
                 1141.96 ± 330.11 
                 1460.67 ± 577.64 
               
               
                 signal transducer 
                   
                   
                   
                   
                   
               
               
                 and activator of 
                   
                   
                   
                   
                   
               
               
                 transcription 1 
                   
                   
                   
                   
                   
               
               
                 SCYE1 
                 1.27 
                 0.0103 
                 0.041 
                  278.24 ± 100.74 
                  352.82 ± 149.81 
               
               
                 small inducible 
                   
                   
                   
                   
                   
               
               
                 cytokine 
                   
                   
                   
                   
                   
               
               
                 subfamily E, 
                   
                   
                   
                   
                   
               
               
                 member 1 
                   
                   
                   
                   
                   
               
               
                 IL-7R 
                 0.80, 
                 0.0061, 
                 0.0406, 
                 5219.38 ± 1457.55, 
                 4291.38 ± 1772.32, 
               
               
                 interleukin 7 
                 0.74 
                 0.0011 
                 0.0375 
                 3309.89 ± 1296.32 
                 2467.81 ± 1094.22 
               
               
                 receptor 
                   
                   
                   
                   
                   
               
               
                 IL-2Ra 
                 0.69 
                 0.01811 
                 0.0544 
                  113.56 ± 58.89 
                  79.12 ± 39.28 
               
               
                 interleukin 2 
                   
                   
                   
                   
                   
               
               
                 receptor, a 
                   
                   
                   
                   
                   
               
               
                 IL-15 
                 0.74 
                 0.0032 
                 0.0398 
                  54.76 ± 39.59 
                  40.63 ± 31.09 
               
               
                 interleukin 15 
                   
                   
                   
                   
                   
               
               
                 TNFSF8 
                 0.73 
                 0.0202 
                 0.0605 
                  248.08 ± 148.25 
                  181.53 ± 92.03 
               
               
                 tumor necrosis 
                   
                   
                   
                   
                   
               
               
                 factor 
                   
                   
                   
                   
                   
               
               
                 superfamily, 
                   
                   
                   
                   
                   
               
               
                 member 8 
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Chemokines-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 CCR3 
                 1.52 
                 0.0092 
                 0.041 
                  19.73 ± 5.59 
                  29.92 ± 13.46 
               
               
                 Chemokine (C-C 
                   
                   
                   
                   
                   
               
               
                 motif) receptor 3 
                   
                   
                   
                   
                   
               
               
                 ITGB1 
                 1.29, 
                 0.0055, 
                 0.0405, 
                 1449.91 ± 772.02, 
                 1867.82 ± 802.10, 
               
               
                 integrin, b1 
                 1.20 
                 0.0012 
                 0.0375 
                  396.68 ± 336.59 
                  476.25 ± 376.51 
               
               
                 (antigen CD29) 
                   
                   
                   
                   
                   
               
               
                 CXCR3 
                 1.29 
                 0.0286 
                 0.0825 
                  47.04 ± 23.35 
                  60.53 ± 33.18 
               
               
                 chemokine 
                   
                   
                   
                   
                   
               
               
                 receptor 3 
                   
                   
                   
                   
                   
               
               
                 CXCR7 
                 0.75 
                 0.0205 
                 0.0614 
                  83.62 ± 25.83 
                  63.45 ± 25.78 
               
               
                 chemokine 
                   
                   
                   
                   
                   
               
               
                 receptor 7 
                   
                   
                   
                   
                   
               
               
                 ITGA6 
                 0.72 
                 0.0211 
                   
                   
                   
               
               
                 integrin, a6 
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Activation, proliferation and differentiation-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 MKI67 
                 1.98, 
                 0.0165, 
                 0.0498, 
                  41.58 ± 22.80, 
                  82.16 ± 62.67, 
               
               
                 antigen identified 
                 1.69 
                 0.0104 
                 0.0410 
                  19.37 ± 9.50 
                  32.73 ± 20.37 
               
               
                 by monoclonal 
                   
                   
                   
                   
                   
               
               
                 antibody Ki-67 
                   
                   
                   
                   
                   
               
               
                 ICOS 
                 1.49 
                 0.0011 
                 0.0375 
                  587.76 ± 294.69 
                  874.26 ± 397.84 
               
               
                 inducible T-cell 
                   
                   
                   
                   
                   
               
               
                 costimulator 
                   
                   
                   
                   
                   
               
               
                 GATA3 
                 1.43 
                 0.0124 
                 0.042 
                  284.77 ± 189.82 
                  406.03 ± 268.34 
               
               
                 GATA binding 
                   
                   
                   
                   
                   
               
               
                 protein 3 
                   
                   
                   
                   
                   
               
               
                 CTLA-4 
                 1.37, 
                 0.0023, 
                 0.0392, 
                  163.55 ± 83.74, 
                  224.53 ± 114.73, 
               
               
                 cytotoxic T- 
                 1.37 
                 0.0249 
                 0.0734 
                  511.58 ± 260.28 
                  700.23 ± 359.54 
               
               
                 lymphocyte- 
                   
                   
                   
                   
                   
               
               
                 associated protein 
                   
                   
                   
                   
                   
               
               
                 4 
                   
                   
                   
                   
                   
               
               
                 CD38 
                 1.36 
                 0.0075 
                 0.0408 
                  32.73 ± 13.37 
                  44.62 ± 20.04 
               
               
                 CD38 molecule 
                   
                   
                   
                   
                   
               
               
                 MICA 
                 0.7  
                 0.0015 
                 0.0379 
                  120.33 ± 67.61 
                  84.36 ± 51.72 
               
               
                 MHC class I 
                   
                   
                   
                   
                   
               
               
                 polypeptide- 
                   
                   
                   
                   
                   
               
               
                 related sequence 
                   
                   
                   
                   
                   
               
               
                 A 
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 BCL family-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 Bcl3 
                 1.56 
                 0.0036 
                 0.0400 
                  199.55 ± 112.65 
                  311.36 ± 120.66 
               
               
                 B-cell 
                   
                   
                   
                   
                   
               
               
                 CLL/lymphoma 3 
                   
                   
                   
                   
                   
               
               
                 BAK1 
                 1.26 
                 2.00E−04 
                 0.0365 
                  130.50 ± 27.61 
                  164.92 ± 33.45 
               
               
                 Bcl2- 
                   
                   
                   
                   
                   
               
               
                 antagonist/killer 1 
                   
                   
                   
                   
                   
               
               
                 Bcl2L11 
                 1.24, 
                 0.0067, 
                 0.0407, 
                  314.36 ± 77.54, 
                  388.54 ± 79.60, 
               
               
                 Bcl2-like 11 
                 1.21 
                 0.0369 
                 0.1053 
                  442.48 ± 299.18 
                  536.88 ± 388.57 
               
               
                 BCLAF1 
                 1.21 
                 0.0234 
                 0.0695 
                  61.14 ± 14.91 
                  73.89 ± 26.64 
               
               
                 Bcl2-associated 
                   
                   
                   
                   
                   
               
               
                 transcription 
                   
                   
                   
                   
                   
               
               
                 factor 1 
                   
                   
                   
                   
                   
               
               
                 Bcl2 
                 0.79, 
                 0.0246, 
                 0.0726, 
                  617.68 ± 266.67, 
                  492.80 ± 272.63, 
               
               
                 B-cell 
                 0.72, 
                 0.0158, 
                 0.0479, 
                  189.15 ± 81.00, 
                  137.91 ± 40.11, 
               
               
                 CLL/lymphoma 2 
                 0.63 
                 0.0050 
                 0.0404 
                  427.68 ± 327.43 
                  272.97 ± 207.62 
               
            
           
           
               
            
               
                 Apoptosis MAP kinase and protein kinase-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 ANXA5 
                 1.32 
                 0.0271 
                 0.0794 
                  599.68 ± 269.08 
                  793.89 ± 425.06 
               
               
                 annexin 5 
                   
                   
                   
                   
                   
               
               
                 MAPK6 
                 1.31 
                 0.0261 
                 0.0768 
                  209.49 ± 142.30 
                  273.63 ± 202.36 
               
               
                 mitogen-activated 
                   
                   
                   
                   
                   
               
               
                 protein kinase 6 
                   
                   
                   
                   
                   
               
               
                 PPP1CC 
                 1.23 
                 0.0152 
                 0.0462 
                  998.56 ± 432.59 
                 1225.87 ± 571.01 
               
               
                 protein 
                   
                   
                   
                   
                   
               
               
                 phosphatase 1, 
                   
                   
                   
                   
                   
               
               
                 catalytic subunit, 
                   
                   
                   
                   
                   
               
               
                 g isoform 
                   
                   
                   
                   
                   
               
               
                 CASP7 
                 1.22 
                 0.0145 
                 0.0441 
                  141.11 ± 41.72 
                  171.83 ± 48.77 
               
               
                 caspase 7, 
                   
                   
                   
                   
                   
               
               
                 apoptosis-related 
                   
                   
                   
                   
                   
               
               
                 cysteine 
                   
                   
                   
                   
                   
               
               
                 peptidase 
                   
                   
                   
                   
                   
               
               
                 MAP2K6 
                 0.77 
                 0.0105 
                 0.0410 
                  77.3 ± 41.24 
                  59.77 ± 33.17 
               
               
                 mitogen-activated 
                   
                   
                   
                   
                   
               
               
                 protein kinase 
                   
                   
                   
                   
                   
               
               
                 kinase kinase 6 
                   
                   
                   
                   
                   
               
               
                 ANXA11 
                 0.75 
                 0.0003 
                 0.0368 
                  93.23 ± 20.45 
                  70.21 ± 31.98 
               
               
                 annexin 11 
                   
                   
                   
                   
                   
               
               
                 ATF7 
                 0.75 
                 0.0252 
                 0.0743 
                  170.44 ± 120.64 
                  128.30 ± 107.21 
               
               
                 activating 
                   
                   
                   
                   
                   
               
               
                 transcription 
                   
                   
                   
                   
                   
               
               
                 factor 7 
                   
                   
                   
                   
                   
               
               
                 MAP4K4 
                 0.63 
                 0.0095 
                 0.041 
                  137.49 ± 51.60 
                  86.97 ± 29.96 
               
               
                 mitogen-activated 
                   
                   
                   
                   
                   
               
               
                 protein kinase 
                   
                   
                   
                   
                   
               
               
                 kinase kinase 4 
                   
                   
                   
                   
                   
               
               
                 CASP10 
                 0.62 
                 0.004 
                 0.0401 
                  62.63 ± 27.12 
                  39.11 ± 15.88 
               
               
                 caspase 10, 
                   
                   
                   
                   
                   
               
               
                 apoptosis-related 
                   
                   
                   
                   
                   
               
               
                 cysteine 
                   
                   
                   
                   
                   
               
               
                 peptidase 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2b 
               
             
            
               
                   
               
               
                 genes impacted by ipilimumab in CD8+ T cells 
               
            
           
           
               
               
               
               
               
               
            
               
                 Gene symbol 
                   
                   
                   
                   
                   
               
               
                 Gene name 
                 Fold change 
                 p-value 
                 FDR 
                 Pre-Mean ± SD 
                 Post-Mean ± SD 
               
               
                   
               
            
           
           
               
            
               
                 Cell cycle-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 CDCA2 
                 1.95 
                 0.0189 
                 0.0647 
                  12.86 ± 6.07 
                  25.11 ± 16.20 
               
               
                 cell division cycle 
                   
                   
                   
                   
                   
               
               
                 associated 2 
                   
                   
                   
                   
                   
               
               
                 CDC2 
                 1.81, 
                 0.0300, 
                 0.0897, 
                  18.37 ± 6.09, 
                  33.23 ± 21.15, 
               
               
                 cell division cycle 2,  
                 1.64 
                 0.0274 
                 0.0827 
                  20.92 ± 6.16 
                  34.31 ± 21.02 
               
               
                 G1 to S and G2 to M 
                   
                   
                   
                   
                   
               
               
                 CDCA7 
                 1.67 
                 0.0113 
                 0.0647 
                  87.63 ± 93.37 
                 146.50 ± 111.23 
               
               
                 Cell division cycle 
                   
                   
                   
                   
                   
               
               
                 associated 7 
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Cytokines-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 TNFSF4 
                 0.58 
                 0.0082 
                 0.0647 
                  40.88 ± 27.10 
                  23.93 ± 14.71 
               
               
                 tumor necrosis factor 
                   
                   
                   
                   
                   
               
               
                 superfamily, member 4 
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Chemokines-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 CXCR3 
                 1.25 
                 0.0036 
                 0.0647 
                  54.98 ± 21.50 
                  68.96 ± 21.37 
               
               
                 chemokine (C-X-C 
                   
                   
                   
                   
                   
               
               
                 motif) receptor 3 
                   
                   
                   
                   
                   
               
               
                 ITGAV 
                 0.80 
                 5.00E−04 
                 0.0647 
                  114.65 ± 54.10 
                  91.78 ± 42.91 
               
               
                 integrin, aV (antigen 
                   
                   
                   
                   
                   
               
               
                 CD51) 
                   
                   
                   
                   
                   
               
               
                 CXCR7 
                 0.73 
                 0.0268 
                 0.081 
                  92.65 ± 43.71 
                  68.45 ± 33.28 
               
               
                 chemokine (C-X-C 
                   
                   
                   
                   
                   
               
               
                 motif) receptor 7 
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Activation Differentiation and Interaction-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 HLA-DRB4 
                 1.4 
                 0.0163 
                 0.0647 
                  28.04 ± 26.29 
                  39.18 ± 37.40 
               
               
                 major 
                   
                   
                   
                   
                   
               
               
                 histocompatibility 
                   
                   
                   
                   
                   
               
               
                 complex, class II, DR 
                   
                   
                   
                   
                   
               
               
                 b4 
                   
                   
                   
                   
                   
               
               
                 GATA3 
                 1.39, 
                 0.0100, 
                 0.0647, 
                  178.84 ± 104.07, 
                 248.93 ± 148.36, 
               
               
                 GATA binding protein 
                 1.35 
                 0.0026 
                 0.0647 
                  139.84 ± 44.14 
                 188.83 ± 75.51 
               
               
                 3 
                   
                   
                   
                   
                   
               
               
                 HLA-DRA 
                 1.38 
                 0.0193 
                 0.0647 
                  540.53 ± 402.57 
                 745.47 ± 350.32 
               
               
                 major 
                   
                   
                   
                   
                   
               
               
                 histocompatibility 
                   
                   
                   
                   
                   
               
               
                 complex, class II, DR a 
                   
                   
                   
                   
                   
               
               
                 CD6 
                 1.32 
                 0.0248 
                 0.0754 
                  179.47 ± 95.79 
                 236.58 ± 157.76 
               
               
                 CD6 molecule (CD166 
                   
                   
                   
                   
                   
               
               
                 receptor) 
                   
                   
                   
                   
                   
               
               
                 CD5 
                 1.22 
                 0.0181 
                 0.0647 
                  103.58 ± 38.21 
                 126.47 ± 45.17 
               
               
                 CD5 molecule 
                   
                   
                   
                   
                   
               
               
                 CD209 
                 1.20 
                 0.0288 
                 0.0865 
                  36.73 ± 4.88 
                  44.08 ± 7.60 
               
               
                 CD209 molecule 
                   
                   
                   
                   
                   
               
               
                 EOMES 
                 0.69 
                 0.0292 
                 0.0877 
                 1063.26 ± 642.42 
                 738.05 ± 445.38 
               
               
                 Eomesodermin 
                   
                   
                   
                   
                   
               
            
           
           
               
            
               
                 Apoptosis, MAP Kinases-related 
               
            
           
           
               
               
               
               
               
               
            
               
                 TIAM1 
                 1.68 
                 0.0105 
                 0.0647 
                  83.63 ± 67.00 
                 140.61 ± 116.68 
               
               
                 T-cell lymphoma 
                   
                   
                   
                   
                   
               
               
                 invasion and metastasis 
                   
                   
                   
                   
                   
               
               
                 1 
                   
                   
                   
                   
                   
               
               
                 ANXA5 
                 1.31 
                 0.0114 
                 0.0647 
                  576.93 ± 278.33 
                 747.53 ± 316.78 
               
               
                 annexin 5 
                   
                   
                   
                   
                   
               
               
                 API5 
                 1.24 
                 0.0088 
                 0.0647 
                  187.92 ± 118.48 
                 233.04 ± 151.93 
               
               
                 apoptosis inhibitor 5 
               
               
                   
               
            
           
         
       
     
     Pharmacodynamic Effects of Ipilimumab on T Cells 
     To verify changes in selected molecules from the microarray analysis and further investigate the mechanism of action of ipilimumab, a flow cytometry study was undertaken with the pre-, 3-month and 6-month post-ipilimumab PBMCs from expanded groups of 25 and 37 patients respectively (Table 6b, 6c) overlapping the microarray cohort of 12. In addition to the selected immunological biomarkers, CD4 +  and CD8 +  T cell effector-memory/naive phenotypes were also measured. Biomarkers with MFI and frequency both significantly changed in absolute value and fold change with p≤0.02 by Wilcoxon and also significant by paired Student T-test. 
     
       
         
           
               
             
               
                 TABLE 6b 
               
             
            
               
                   
               
               
                 Demographics of patients with 3-month measurements (n = 25) 
               
            
           
           
               
               
               
               
            
               
                   
                 Variable 
                 Level 
                 n (%) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 gender 
                 F 
                 9 
                 (36.0) 
               
               
                   
                   
                 M 
                 16 
                 (64.0) 
               
               
                   
                 stage 
                 III 
                 10 
                 (40.0) 
               
               
                   
                   
                 IV 
                 15 
                 (60.0) 
               
               
                   
                 HLA A2 
                 A2+ 
                 18 
                 (72.0) 
               
               
                   
                   
                 A2− 
                 7 
                 (28.0) 
               
               
                   
                 dosage 
                 10 mg/kg 
                 16 
                 (64.0) 
               
               
                   
                   
                  3 mg/kg 
                 9 
                 (36.0) 
               
               
                   
                 irAE 
                 N 
                 15 
                 (60.0) 
               
               
                   
                   
                 Y 
                 10 
                 (40.0) 
               
               
                   
                 Outcome 
                 NED 
                 17 
                 (68.0) 
               
               
                   
                   
                 Relapse 
                 8 
                 (32.0) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6c 
               
             
            
               
                   
               
               
                 Demographics of patients with 6-month measurements (n = 37) 
               
            
           
           
               
               
               
               
            
               
                   
                 Variable 
                 Level 
                 n (%) 
               
               
                   
               
               
                   
                 gender 
                 F 
                 12 (32.4) 
               
               
                   
                   
                 M 
                 25 (67.6) 
               
               
                   
                 stage 
                 III 
                 17 (45.9) 
               
               
                   
                   
                 IV 
                 20 (54.1) 
               
               
                   
                 HLA A2 
                 A2+ 
                 20 (54.1) 
               
               
                   
                   
                 A2− 
                 17 (45.9) 
               
               
                   
                 dosage 
                 10 mg/kg 
                 31 (83.8) 
               
               
                   
                   
                  3 mg/kg 
                  6 (16.2) 
               
               
                   
                 irAE 
                 N 
                 23 (62.2) 
               
               
                   
                   
                 Y 
                 14 (37.8) 
               
               
                   
                 Outcome 
                 NED 
                 27 (73.0) 
               
               
                   
                   
                 Relapse 
                 10 (27.0) 
               
               
                   
               
            
           
         
       
     
     Increased Ki67 Expression in, and ICOS on CD4 |  and CD8 |  T Cells 3- and 6-Months after Ipilimumab 
     The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation (Scholzen T, et al.  J Cell Physiol.  182:311-22 (2000)) and cell cycling (Hertoghs K M, et al.  J Clin Invest.  120:4077-90 (2010)) and is an indicator of the growth fraction of a given cell population. As demonstrated in Table 3a and Table 3b, the MFI and frequency of Ki67 in CD4 +  and CD8 +  T cells was significantly increased in both 3-month (Table 3a) and 6-month (Table 3b) post-treatment PBMCs with p≤0.0032 for all by Wilcoxon. 
     
       
         
           
               
             
               
                 TABLE 3a 
               
             
            
               
                   
               
               
                 Statistical analysis of changes in biomarkers at 3 months 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                 Median 
                   
                 Median fold 
                   
               
               
                   
                   
                 change* 
                 Wilcoxon 
                 change* 
                 Wilcoxon 
               
               
                 Biomarker 
                 n 
                 (Q1, Q3) 
                 p 
                 (Q1, Q3) 
                 p 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 MFI-ICOS-CD4 
                 25 
                    2.10.00 
                 &lt;.0001 
                 1.41 
                 &lt;.0001 
               
               
                   
                   
                  (71.00, 273.00) 
                   
                 (0.60, 2.14) 
                   
               
               
                 Frequency-ICOS- 
                 25 
                 13.00 
                 &lt;.0001 
                 1.35 
                 &lt;.0001 
               
               
                 CD4 
                   
                  (1.15, 20.69) 
                   
                 (0.22, 3.62) 
                   
               
               
                 MFI-ICOS-CD8 
                 25 
                 34.90 
                 &lt;.0001 
                 0.56 
                 &lt;.0001 
               
               
                   
                   
                  (4.60, 46.10) 
                   
                 (0.09, 1.12) 
                   
               
               
                 Frequency-ICOS- 
                 25 
                  4.19 
                 &lt;.0001 
                 2.90 
                 &lt;.0001 
               
               
                 CD8 
                   
                 (2.07, 7.75) 
                   
                 (1.25, 6.71) 
                   
               
               
                 MFI-Ki67-CD4 
                 24 
                 779.50  
                 &lt;.0001 
                 0.58 
                 &lt;.0001 
               
               
                   
                   
                  (178.50, 1657.00) 
                   
                 (0.12, 1.43) 
                   
               
               
                 Frequency-Ki67- 
                 24 
                  3.62 
                 &lt;.0001 
                 1.49 
                 &lt;.0001 
               
               
                 CD4 
                   
                 (0.74, 6.94) 
                   
                 (0.18, 2.63) 
                   
               
               
                 MFI-Ki67-CD8 
                 24 
                 480.00  
                 0.0032 
                 0.32 
                 0.0011 
               
               
                   
                   
                   (−6.00, −1268.00) 
                   
                 (−0.00, 1.23)  
                   
               
               
                 Frequency-Ki67- 
                 24 
                  1.49 
                 0.0009 
                 0.54 
                 0.0003 
               
               
                 CD8 
                   
                 (−0.10, 4.86)  
                   
                 (−0.03, 2.20)  
                   
               
               
                 MFI-CCR7-CD8 
                 21 
                 −279.00  
                 0.0003 
                 −0.23  
                 0.0004 
               
               
                   
                   
                 (−645.00, 131.00)  
                   
                 (−0.30, 0.12)  
                   
               
               
                 Frequency-CCR7- 
                 25 
                 −3.98 
                 0.0122 
                 −0.14  
                 0.018 
               
               
                 CD8 
                   
                 (−11.40, 1.30)  
                   
                 (−0.27, 0.04)  
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3b 
               
             
            
               
                   
               
               
                 Statistical analysis of changes in biomarkers at 6 months 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                 Median 
                   
                 Median fold 
                   
               
               
                   
                   
                 change* 
                 Wilcoxon 
                 change* 
                 Wilcoxon 
               
               
                 Biomarker 
                 n 
                 (Q1, Q3) 
                 p 
                 (Q1, Q3) 
                 p 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 MFI-ICOS-CD4 
                 37 
                 111.00  
                 &lt;.0001 
                 0.84 
                 &lt;.0001 
               
               
                   
                   
                  (46.80, 166.00) 
                   
                 (0.41, 1.06) 
                   
               
               
                 Frequency-ICOS- 
                 37 
                 9.45 
                 &lt;.0001 
                 1.66 
                 &lt;.0001 
               
               
                 CD4 
                   
                  (3.13, 14.57) 
                   
                 (0.70, 2.42) 
                   
               
               
                 MFI-ICOS-CD8 
                 37 
                 29.10  
                 &lt;.0001 
                 0.47 
                 &lt;.0001 
               
               
                   
                   
                 (11.80, 44.50) 
                   
                 (0.21, 0.64) 
                   
               
               
                 Frequency-ICOS- 
                 37 
                 3.07 
                 &lt;.0001 
                 1.34 
                 &lt;.0001 
               
               
                 CD8 
                   
                 (1.62, 5.36) 
                   
                 (0.63, 3.30) 
                   
               
               
                 MFI-Ki67-CD4 
                 36 
                 535.50  
                 &lt;.0001 
                 0.49 
                 &lt;.0001 
               
               
                   
                   
                  (207.50, 1517.50) 
                   
                 (0.24, 1.16) 
                   
               
               
                 Frequency-Ki67- 
                 35 
                 2.33 
                 &lt;.0001 
                 0.87 
                 &lt;.0001 
               
               
                 CD4 
                   
                 (1.37, 6.97) 
                   
                 (0.29, 2.12) 
                   
               
               
                 MFI-Ki67-CD8 
                 36 
                 326.00  
                 &lt;.0001 
                 0.30 
                 &lt;.0001 
               
               
                   
                   
                  (85.50, 770.50) 
                   
                 (0.10, 0.70) 
                   
               
               
                 Frequency-Ki67- 
                 36 
                 1.88 
                 0.0004 
                 0.55 
                 &lt;.0001 
               
               
                 CD8 
                   
                 (0.13, 4.91) 
                   
                 (0.04, 1.52) 
                   
               
               
                 MFI-Gata3-CD4 
                 28 
                 78.00  
                 0.004 
                 0.21 
                 0.0038 
               
               
                   
                   
                  (8.00, 189.50) 
                   
                 (−0.02, 0.43)  
                   
               
               
                 Frequency-Gata3- 
                 28 
                 3.76 
                 0.0004 
                 0.94 
                 &lt;.0001 
               
               
                 CD4 
                   
                 (0.28, 8.04) 
                   
                 (−0.01, 1.62)  
                   
               
               
                 MFI-Gata3-CD8 
                 28 
                 76.50  
                 0.0045 
                 0.16 
                 0.0032 
               
               
                   
                   
                  (8.00, 119.50) 
                   
                 (0.03, 0.35) 
                   
               
               
                 Frequency-Gata3- 
                 28 
                 2.07 
                 0.0006 
                 0.59 
                 &lt;.0001 
               
               
                 CD8 
                   
                 (0.59, 4.02) 
                   
                 (0.09, 1.23) 
                   
               
               
                 MFI-CCR7-CD4 
                 37 
                 −290.00   
                 &lt;.0001 
                 −0.14  
                 &lt;.0001 
               
               
                   
                   
                 (−587.00, −113.00) 
                   
                 (−0.21, −0.07) 
                   
               
               
                 Frequency-CCR7- 
                 37 
                 −2.90  
                 0.0017 
                 −0.03  
                 0.006 
               
               
                 CD4 
                   
                 (−6.80, −0.40) 
                   
                 (−0.09, 0.01)  
                   
               
               
                 MFI-IL-7R-CD4 
                 37 
                 −23.00  
                 0.0001 
                 −0.08  
                 &lt;.0001 
               
               
                   
                   
                 (−45.00, −7.00)  
                   
                 (−0.17, 0.02)  
                   
               
               
                 Frequency-IL-7R- 
                 37 
                 −1.70  
                 0.0093 
                 −0.15  
                 0.0271 
               
               
                 CD4 
                   
                 (−3.20, 0.36)  
                   
                 (−0.34, 0.05)  
               
               
                   
               
            
           
         
       
     
     Inducible T-cell co-stimulator (ICOS) is a T cell surface molecule structurally related to CD28 and CTLA-4 (Dong C, et al.  Nature.  409:97-101 (2001)). It is expressed at low levels on resting naïve T cells and is rapidly up-regulated following TCR ligation and CD28 costimulation (McAdam A J, et al.  J Immunol  165:5035-40 (2000)). After ipilimumab treatment, the MFI and frequency of ICOS was increased significantly on CD4 +  and CD8 +  T cells, with higher increases on CD4 +  than on CD8 +  T cells and higher increases at 3-month than at 6-month post treatment (Table 3). 
     Decreased CCR7 on CD8 |  and IL-7R Expression on CD4 +  Tcells after Ipilimumab 
     The MFI and frequency of CCR7 expression on CD8 +  T cells was decreased at 3- and 6-month post-ipilimumab (Table 3). The MFI of IL-7R on CD4 +  T cells was down-regulated at 3-month and 6-month post-ipilimumab treatment, and the frequency of IL-7R on CD4+ T cells was significantly decreased at 6-month post-ipilimumab treatment (Table 3). 
     Increased GATA3 Expression in CD4 +  and CD8 +  T Cells 
     GATA3 is a transcription factor that is a marker for Th2 polarization and is associated with the generation of Th2 cytokines IL-4, IL-5 and IL-10. After treatment with ipilimumab, GATA3 expression as MFI and frequency was increased significantly in CD4 |  and CD8 |  T cells at 6-month post-ipilimumab treatment (Table 3b). 
     Surrogate Biomarkers on/in T Cells Associated with Relapse or irAE 
     In an univariate logistic regression analysis using changes in expression by flow cytometry as a continuous variable one at a time, only absolute decrease in Ki67 + EOMES + CD4 + , in MFI and frequency of CCR7 + CD8 + , and increase in MFI of TGFβR3 on CD8 + , in MFI and frequency of EOMES + CD8 +  and in GranzymeB + EOMES + CD8 +  T cells, Perforin + EOMES + CD8 +  T cells, and CTLA-4 + CD4 +  T cells were associated with a higher likelihood of relapse with p&lt;0.05 (Table 4a). Only absolute decrease of Ki67 + CD8 +  T cells, CD71 + CD8 +  T cells, ICOS + CD8 +  T cells, and PD-1 + CD8 +  T cells were associated with a development of irAE, (Table 4b) with p&lt;0.05. 
     
       
         
           
               
             
               
                 TABLE 4a 
               
             
            
               
                   
               
               
                 Association of changes in biomarkers at 6 months with outcome 
               
            
           
           
               
               
               
               
            
               
                   
                 Variable 
                 Slope 
                 p-value 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                   
                 Ki67 EOMES CD4 (%) 
                 −3.5232 
                 0.0285 
               
               
                   
                 MFI CCR7 CD8 
                 −0.0021 
                 0.0158 
               
               
                   
                 CCR7 CD8 (%) 
                 −0.0947 
                 0.0379 
               
               
                   
                 EOMES CD8 (%) 
                 0.1758 
                 0.0072 
               
               
                   
                 MFI EOMES CD8 
                 0.0025 
                 0.0282 
               
               
                   
                 MFI TGFbRS CD8 
                 0.0049 
                 0.0337 
               
               
                   
                 EOMES Perforin CD8 (%) 
                 0.2109 
                 0.0278 
               
               
                   
                 EOMES Granzyme B CD8 (%) 
                 0.2126 
                 0.0078 
               
               
                   
                 CTLA-4 CD4 (%) 
                 0.4222 
                 0.0178 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4b 
               
             
            
               
                   
               
               
                 Association of changes in biomarkers with irAE (Yes vs. No) 
               
            
           
           
               
               
               
               
            
               
                   
                 Variable 
                 Slope 
                 p-value 
               
               
                   
               
               
                   
                 Ki67 CD8 (%) 
                 −0.3167 
                 0.0217 
               
               
                   
                 CD71 CD8 
                 −0.5475 
                 0.0484 
               
               
                   
                 CD71 CD8 (%) 
                 −0.0075 
                 0.0297 
               
               
                   
                 ICOS CD8 (%) 
                 −0.0049 
                 0.0411 
               
               
                   
                 PD-1 CD8 (%) 
                 −0.0293 
                 0.0476 
               
               
                   
               
               
                 (%) means percentage of CD4 +  or CD8 +  T cells (as indicated) that had the indicated markers. 
               
               
                 MFI means the mean fluorescence intensity of the indicated marker in the indicated type of T cell (CD4 +  or CD8 +).   
               
            
           
         
       
     
     Pre-Treatment Biomarkers on/in T Cells Associated with Outcome and irAE 
     An analysis dichotomized by the median of baseline biomarkers revealed that low Ki67 + EOMES + CD8 + , high MFI of CCR7 + CD4 +  and low EOMES + CD8 +  were significantly associated with relapse (Table 5a) with p values of 0.0008, 0.0190 and 0.0242 respectively. These pre-treatment biomarkers collected from a cohort of 55 patients were also confirmed in a univariable logistic regression analysis. Another analysis of covariate dichotomized by the median value for baseline biomarkers showed that low Ki67 + EOMES + CD4 +  and high MFI of TGFβR3 on CD8 +  T cells were associated with occurrence of irAE (Table 5b) with p=0.0046 and p=0.0218 respectively. 
     
       
         
           
               
             
               
                 TABLE 5a 
               
             
            
               
                   
               
               
                 Association between outcome and dichotomized baseline biomarkers 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Chi- 
               
               
                   
                 Out- 
                 Covariate 
                   
                 Odds Ratio 
                 Square 
               
               
                 Association 
                 come 
                 Level 
                 n (%) 
                 (95% CI) 
                 p-value 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Ki67EOMES CD8 
                 Relapse 
                 ≤2.11 
                 15 
                 (75.0) 
                 11.25 
                 0.0008 
               
               
                 Outcome 
                 NED 
                 ≤2.11 
                 5 
                 (25.0) 
                  (2.52, 50.27) 
                   
               
               
                   
                 Relapse 
                 &gt;2.11 
                 4 
                 (21.1) 
                   
                   
               
               
                   
                 NED 
                 &gt;2.11 
                 15 
                 (78.9) 
                   
                   
               
               
                 MFI CCR7 CD4 
                 Relapse 
                 ≤2402 
                 6 
                 (21.4) 
                 0.25 
                 0.019 
               
               
                 Outcome 
                 NED 
                 ≤2402 
                 22 
                 (78.6) 
                 (0.08, 0.82) 
                   
               
               
                   
                 Relapse 
                 &gt;2402 
                 14 
                 (51.9) 
                   
                   
               
               
                   
                 NED 
                 &gt;2402 
                 13 
                 (48.1) 
                   
                   
               
               
                 Frequency EOMES 
                 Relapse 
                 ≤55.6 
                 14 
                 (51.9) 
                 3.77 
                 0.0242 
               
               
                 CD8 Outcome 
                 NED 
                 ≤55.6 
                 13 
                 (48.1) 
                  (1.16, 12.27) 
                   
               
               
                   
                 Relapse 
                 &gt;55.6 
                 6 
                 (22.2) 
                   
                   
               
               
                   
                 NED 
                 &gt;55.6 
                 21 
                 (77.8) 
                   
                   
               
               
                 Frequency CD109 
                 Relapse 
                 ≤0.7315 
                 5 
                 (19.2) 
                 0.20 
                 0.0201 
               
               
                 CD8 Outcome 
                 NED 
                 ≤0.7315 
                 21 
                 (80.8) 
                 (0.06, 0.71) 
                   
               
               
                   
                 Relapse 
                 &gt;0.7315 
                 14 
                 (53.8) 
                   
                   
               
               
                   
                 NED 
                 &gt;0.7315 
                 12 
                 (46.2) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5b 
               
             
            
               
                   
               
               
                 Association between irAE (Yes/No) and 
               
               
                 baseline dichotomized biomarkers 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 Chi- 
               
               
                   
                 Out- 
                 Covariate 
                   
                 Odds Ratio 
                 Square 
               
               
                 Association 
                 come 
                 Level 
                 n (%) 
                 (95% CI) 
                 p-value 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Ki67EOMES 
                 Y 
                 ≤0.446 
                 12 
                 (60.0) 
                 8.00 
                 0.0046 
               
               
                 CD4 irAE 
                 N 
                 ≤0.446 
                 8 
                 (40.0) 
                 (1.74, 36.70) 
                   
               
               
                   
                 Y 
                 &gt;0.446 
                 3 
                 (15.8) 
                   
                   
               
               
                   
                 N 
                 &gt;0.446 
                 16 
                 (84.2) 
                   
                   
               
               
                 MFI TGFbR3 
                 Y 
                 ≤527 
                 8 
                 (28.6) 
                 0.28 
                 0.0218 
               
               
                 CD8 irAE 
                 N 
                 ≤527 
                 20 
                 (71.4) 
                 (0.09, 0.85)  
                   
               
               
                   
                 Y 
                 &gt;527 
                 16 
                 (59.3) 
                   
                   
               
               
                   
                 N 
                 &gt;527 
                 11 
                 (40.7) 
                   
                   
               
               
                 Frequency 
                 Y 
                 ≤2.79 
                 18 
                 (69.2) 
                 7.50 
                 0.0019 
               
               
                 CD71 
                 N 
                 ≤2.79 
                 8 
                 (30.8) 
                 (2.18, 25.80) 
                   
               
               
                 CD4 irAE 
                 Y 
                 &gt;2.79 
                 6 
                 (23.1) 
                   
                   
               
               
                   
                 N 
                 &gt;2.79 
                 20 
                 (76.9) 
               
               
                   
               
            
           
         
       
     
     This analysis pointed out the potential importance of EOMES, a transcription factor in the T-box family and involved in the regulation of INF-γ, granzyme B and perforin production by CD8 +  T cells (Pearce E L, et al.  Science.  302:1041-3 (2003)). To better understand the potential role of EOMES + CD8 +  T cells in ipilimumab treatment, pre-treatment specimens were stratified by the median frequency of EOMES +  in CD8 +  T cells. High baseline frequency of EOMES +  in CD8 +  T cells were significantly associated with an improved relapse free survival (RFS) compared with a lower basal level of EOMES + CD8 +  T cells (P=0.0183; log-rank test) ( FIG. 1A ). The patients were stratified by the median frequency of Ki67 + EOMES + CD8 + . The patients with a higher proportion of Ki67 + EOMES + CD8 +  T cells had significantly improved RFS compared with those patients with lower frequency of Ki67 + EOMES + CD8 +  cells ( FIG. 1B ), p=0.0004 by the long-rank test. Patients were also stratified by the median MFI of CCR7 on CD4 +  T cells. The patients with lower MFI of CCR7 +  on CD4 +  had a significantly better RFS than the higher expressing patients ( FIG. 1C ), with p value of 0.0278 by the log-rank test. 
     Some of the significant biomarkers were tested on the 11 available normal phereses. Results for the most significant biomarker (EOMES + Ki67 + CD8 + ) on the 11 normals and the melanoma patients (relapse and NED) in this trial is shown in  FIG. 2 . The median of EOMES + Ki67 + CD8 +  T cells for the 11 normal was 1.57, very similar to the median for the relapse group patients which was 1.50. For the NED group, the median of EOMES + Ki67 + CD8 +  T cells was 2.49 significantly higher than median of relapsed patients (p=0.03, student T test). 
     Biomarker levels associated with cancer prognosis and CTLA-4 blockade treatment are shown in Table 7. The range in the last column is the range of values for that biomarker seen in the group of patients. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Biomarker Levels Associated with Cancer 
               
               
                 Prognosis and CTLA-4 Blockade Treatment 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Range for 
               
               
                   
                   
                   
                 Cut off 
                 total 
               
               
                 Biomarkers 
                 Association 
                 Unit 
                 Value 
                 patients 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Ki67+EOMES+CD8+ 
                 outcome-NED 
                 % 
                 &gt;2.11 
                 5.77 
               
               
                 EOMES+CD8+ 
                 outcome-NED 
                 % 
                 &gt;55.6 
                 67.8 
               
               
                 MFI-CCR7-CD4+ 
                 outcome-NED 
                 MFI 
                 ≤2402 
                 3080 
               
               
                 Ki67+EOMES+CD4+ 
                 irAE-No 
                 % 
                 &gt;0.446 
                 1.96 
               
               
                 MFI-TGFbR3-CD8+ 
                 irAE-No 
                 MFI 
                 ≤527 
                 1711 
               
               
                   
               
               
                 * %: Percentage 
               
               
                 * MFI: Mean Fluorescence Intensity 
               
            
           
         
       
     
     Example 2: Gene Profiling of Melanoma Patients CD8+ T Cells Associated with PD-1 Blockade Treatment and Clinical Outcome 
     PD-1 blocking antibody (BMS-936558) therapy has shown antitumor activity and clinical benefit in melanoma patients. The precise molecular basis and mechanisms of PD-1 blockade in vivo have not been documented and there are few biomarker associated with clinical benefit. The purpose of this study is to understand the mechanisms and investigate potential baseline and surrogate biomarkers associated with clinical benefit of PD-1 blockade in stage IV unresectable melanoma patients&#39; T cells in vivo. 
     Materials and Methods 
     Microarray analysis was performed on flow sorted CD8 +  T cells from PBMCs collected pre- and 12 week-post PD-1 blockade treatment at 1, 3 and 10 mg/kg. The pre-treatment sample was collected within 1 week before anti PD-1 treatment. The post-treatment sample was collected 12 weeks after the initial treatment with 6 injections of anti PD-1 antibody. Stage IIIC and stage IV melanoma patients received intravenous infusion of anti PD-1 (BMS-936558) in dose-escalating ten-patient cohorts at 1, 3, 10 mg/kg. 
     Results 
     To address the molecular mechanisms of PD-1 blockade on human CD8 +  T cells, the molecular signature of PD-1 blockade treatment was characterized at 1, 3, 10 mg/kg by comparing the gene-expression profiles of CD8 +  T cells from pre and post treatment T cells. 
     Gene signatures associated with clinical benefit were assessed by comparing 11 clinical responders to 11 non-responders at baseline, post-treatment at 12 weeks and assessed changes of post vs. pre samples. 
     Results: PD-1 blockade differentially impacts gene expression of CD8 +  T cells at 1, 3 and 10 mg/kg. Baseline and post treatment gene profiles of CD8 +  T cells are significantly associated with clinical benefit ( FIG. 4  (PCA),  FIGS. 5 and 6  (Heat Map)). At base line: low CD276 (B7-H3), high NKTR, PTEN and GATAD1 in CD8 +  T cells are associated with clinical response (Table 9). Increased LAG1, decreased GADD45B, EGR1 and EGR2 are associated with clinical benefit at week 12 post-treatment (Table 8). 
     Baseline, post-treatment, and changes in gene expression profiling of CD8 +  T cells are significantly associated with clinical response after PD-1 blockade. This approach revealed key insights into the biology of PD-1 blockade. Breaking CD8 +  T cell anergy is thus associated with clinical benefit. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Post-treatment Changes in Gene Profile Associated with Response 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Mean Post/ 
               
               
                 Symbol 
                 Gene Title 
                 Fold 
                 pValue 
                 Mean Pre 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 LASS6 
                 LAG1 homolog, 
                 1.30 
                 0.0211 
                 263.9/202.6 
               
               
                   
                 ceramide 
                   
                   
                   
               
               
                   
                 synthase 6 
                   
                   
                   
               
               
                 GADD45G 
                 growth arrest, 
                 0.79 
                 0.0414 
                  90.1/114.0 
               
               
                   
                 DNA-damage- 
                   
                   
                   
               
               
                   
                 inducible, g 
                   
                   
                   
               
               
                 ING1 
                 inhibitor of growth 
                 0.78 
                 0.016 
                 100.4/128.0 
               
               
                   
                 family, member 1 
                   
                   
                   
               
               
                 CKS2 
                 CDC28 protein kinase 
                 0.78 
                 0.0101 
                 345.3/445.0 
               
               
                   
                 regulatory subunit 2 
                   
                   
                   
               
               
                 CDKN1C 
                 Cyclin-dependent kinase 
                 0.78 
                 0.0010 
                 65.8/84.5 
               
               
                   
                 inhibitor 1C 
                   
                   
                   
               
               
                 IFIH1 
                 interferon induced with 
                 0.78 
                 0.0305 
                 38.3/48.9 
               
               
                   
                 helicase C domain 1 
                   
                   
                   
               
               
                 GADD45B 
                 Growth arrest 
                 0.67 
                 0.0003 
                 1851.1/2647.7 
               
               
                   
                 DNA-damage- 
                   
                   
                   
               
               
                   
                 inducible, beta 
                   
                   
                   
               
               
                 IER5 
                 immediate early 
                 0.76 
                 0.0045 
                 3906.4/5155.9 
               
               
                   
                 response 5 
                   
                   
                   
               
               
                 IFNG 
                 interferon, gamma 
                 0.73 
                 0.0232 
                 1062.9/1446.8 
               
               
                 IER5L 
                 immediate early 
                 0.67 
                 0.0207 
                 180.9/271.3 
               
               
                   
                 response 5-like 
                   
                   
                   
               
               
                 EGR2 
                 early growth response 2 
                 0.67 
                 0.046 
                  81.9/121.9 
               
               
                 EGR1 
                 Early growth response 1 
                 0.35 
                 0.0156 
                 1119.0/3218.2 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Baseline Gene Expression Associated with Response 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Mean Responders/ 
               
               
                 Symbol 
                 Gene Title 
                 Fold 
                 pValue 
                 Non Responders 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 USP9Y 
                 ubiquitin specific peptidase 9, 
                 2.23 
                 0.0441 
                 255.2/114.3 
               
               
                   
                 Y-linked 
                   
                   
                   
               
               
                 HSPD1 
                 heat shock 60 kDa protein 1 
                 1.92 
                 0.0415 
                 50.8/26.4 
               
               
                   
                 (chaperonin) 
                   
                   
                   
               
               
                 NSUN6 
                 NOL1/NOP2/Sun domain 
                 1.72 
                 3.04E−05 
                 150.5/87.3  
               
               
                   
                 family, member 6 
                   
                   
                   
               
               
                 CISH 
                 cytokine inducible SH2- 
                 1.7 
                 0.0288 
                 248.1/145.5 
               
               
                   
                 containing protein 
                   
                   
                   
               
               
                 KLF12 
                 Kruppel-like factor 12 
                 1.59 
                 0.0142 
                 192.5/121.3 
               
               
                 NCF4 
                 neutrophil cytosolic factor 4, 
                 1.51 
                 0.049 
                 59.8/39.5 
               
               
                   
                 40 kDa 
                   
                   
                   
               
               
                 SFRS7 
                 splicing factor, 
                 1.49 
                 0.0113 
                 1000.1/670.6  
               
               
                   
                 arginine/serine-rich 7, 35 kDa 
                   
                   
                   
               
               
                 CDC14A 
                 CDC14 cell division cycle 14 
                 1.49 
                 0.0412 
                 144.3/97   
               
               
                   
                 homolog A ( S. cerevisiae ) 
                   
                   
                   
               
               
                 MAP2K5 
                 Mitogen-activated protein 
                 1.48 
                 0.017 
                 77.4/52.4 
               
               
                   
                 kinase kinase 5 
                   
                   
                   
               
               
                 CCAR1 
                 Cell division cycle and 
                 1.46 
                 0.0095 
                 241.3/165.2 
               
               
                   
                 apoptosis regulator 1 
                   
                   
                   
               
               
                 IL11RA 
                 interleukin 11 receptor, alpha 
                 1.46 
                 0.0232 
                 604.3/414.7 
               
               
                 MLL 
                 myeloid/lymphoid or mixed- 
                 1.43 
                 0.0391 
                 36.2/25.3 
               
               
                   
                 lineage leukemia 
                   
                   
                   
               
               
                 HSPBAP1 
                 HSPB (heat shock 27 kDa) 
                 1.42 
                 0.0113 
                 319.2/225.2 
               
               
                   
                 associated protein 1 
                   
                   
                   
               
               
                 MAP4K4 
                 mitogen-activated protein 
                 1.41 
                 0.0242 
                 223.2/158.7 
               
               
                   
                 kinase kinase kinase kinase 4 
                   
                   
                   
               
               
                 HELLS 
                 helicase, lymphoid-specific 
                 1.41 
                 0.0351 
                 107.4/76.0  
               
               
                 SMYD2 
                 SET and MYND domain 
                 1.37 
                 0.0009 
                 168.2/122.6 
               
               
                   
                 containing 2 
                   
                   
                   
               
               
                 C1QTNF3 
                 C1q and tumor necrosis 
                 1.36 
                 0.0242 
                 65.6/48.1 
               
               
                   
                 factor related protein 3 
                   
                   
                   
               
               
                 PTEN 
                 phosphatase and tensin 
                 1.34 
                 0.0232 
                 49.9/37.3 
               
               
                   
                 homolog 
                   
                   
                   
               
               
                 UBE2D1 
                 ubiquitin-conjugating enzyme 
                 1.34 
                 0.0418 
                 77.4/57.8 
               
               
                   
                 E2D 1 
                   
                   
                   
               
               
                 FBXO9 
                 F-box protein 9 
                 1.33 
                 0.006 
                 208.1/156.7 
               
               
                 FAIM3 
                 Fas apoptotic inhibitory 
                 1.33 
                 0.0164 
                 885.2/665.9 
               
               
                   
                 molecule 3 
                   
                   
                   
               
               
                 IFI44 
                 Interferon-induced protein 44 
                 1.33 
                 0.043 
                 91.8/68.9 
               
               
                 NCRNA00153 
                 Non-protein coding RNA 153 
                 1.31 
                 0.0031 
                 146.3/111.8 
               
               
                 NSMAF 
                 Neutral sphingomyelinase 
                 1.3 
                 0.0014 
                 149.4/114.8 
               
               
                   
                 activation associated factor 
                   
                   
                   
               
               
                 H2BFM 
                 H2B histone family, member 
                 1.3 
                 0.0412 
                 100.5/77.5  
               
               
                   
                 M 
                   
                   
                   
               
               
                 GATAD1 
                 GATA zinc finger domain 
                 1.29 
                 0.0412 
                 214.7/166.5 
               
               
                   
                 containing 1 
                   
                   
                   
               
               
                 GATAD1 
                 GATA zinc finger domain 
                 1.27 
                 0.0258 
                 385.9/302.9 
               
               
                   
                 containing 1 
                   
                   
                   
               
               
                 NKTR 
                 natural killer-tumor 
                 1.27 
                 0.0263 
                 2486.9/1951.9 
               
               
                   
                 recognition sequence 
                   
                   
                   
               
               
                 CASP8 
                 caspase 8, apoptosis-related 
                 1.27 
                 0.035 
                 2326.9/1839.1 
               
               
                   
                 cysteine peptidase 
                   
                   
                   
               
               
                 SOCS1 
                 suppressor of cytokine 
                 0.8 
                 0.0182 
                  95.7/119.9 
               
               
                   
                 signaling 1 
                   
                   
                   
               
               
                 P2RY4 
                 pyrimidinergic receptor P2Y, 
                 0.8 
                 0.0005 
                 39.4/49.1 
               
               
                   
                 G-protein coupled, 4 
                   
                   
                   
               
               
                 CD276 
                 CD276 molecule (B7-H3) 
                 0.79 
                 0.0003 
                 108.1/136.9 
               
               
                 RCOR2 
                 REST corepressor 2 
                 0.78 
                 0.0011 
                 156.9/201.4 
               
               
                 PDCD6 
                 Programmed cell death 6 
                 0.77 
                 0.0151 
                  921.4/1198.5 
               
               
                 NUB1 
                 negative regulator of 
                 0.75 
                 0.0054 
                  84.1/111.4 
               
               
                   
                 ubiquitin-like proteins 1 
                   
                   
                   
               
               
                 FCRL2 
                 Fc receptor-like 2 
                 0.71 
                 0.0045 
                 62.1/87.8 
               
               
                 NUCKS1 
                 Nuclear casein kinase, cyclin- 
                 0.69 
                 0.0055 
                 24.5/35.4 
               
               
                   
                 dependent kinase substrate 1 
                   
                   
                   
               
               
                 SHC2 
                 SHC transforming protein 2 
                 0.66 
                 0.0036 
                  98.1/149.1 
               
               
                 SCD5 
                 stearoyl-CoA desaturase 5 
                 0.64 
                 0.0037 
                 211.6/328.3 
               
               
                   
               
            
           
         
       
     
     Disclosed are materials, compositions, and components that can be used for, can be used in conjunction with, can be used in preparation for, or are products of the disclosed method and compositions. These and other materials are disclosed herein, and it is understood that when combinations, subsets, interactions, groups, etc. of these materials are disclosed that while specific reference of each various individual and collective combinations and permutation of these compounds may not be explicitly disclosed, each is specifically contemplated and described herein. For example, if a PD-1 blockade therapy is disclosed and discussed and a number of modifications that can be made to a number of molecules including the active molecule are discussed, each and every combination and permutation of the therapy and molecule and the modifications that are possible are specifically contemplated unless specifically indicated to the contrary. Thus, if a class of molecules A, B, and C are disclosed as well as a class of molecules D, E, and F and an example of a combination molecule, A-D is disclosed, then even if each is not individually recited, each is individually and collectively contemplated. Thus, is this example, each of the combinations A-E, A-F, B-D, B-E, B-F, C-D, C-E, and C-F are specifically contemplated and should be considered disclosed from disclosure of A, B, and C; D, E, and F; and the example combination A-D. Likewise, any subset or combination of these is also specifically contemplated and disclosed. Thus, for example, the subgroup of A-E, B-F, and C-E are specifically contemplated and should be considered disclosed from disclosure of A, B, and C; D, E, and F; and the example combination A-D. Further, each of the materials, compositions, components, etc. contemplated and disclosed as above can also be specifically and independently included or excluded from any group, subgroup, list, set, etc. of such materials. These concepts apply to all aspects of this application including, but not limited to, steps in methods of making and using the disclosed compositions. Thus, if there are a variety of additional steps that can be performed it is understood that each of these additional steps can be performed with any specific embodiment or combination of embodiments of the disclosed methods, and that each such combination is specifically contemplated and should be considered disclosed. 
     Ranges may be expressed herein as from “about” one particular value, and/or to “about” another particular value. When such a range is expressed, also specifically contemplated and considered disclosed is the range from the one particular value and/or to the other particular value unless the context specifically indicates otherwise. Similarly, when values are expressed as approximations, by use of the antecedent “about,” it will be understood that the particular value forms another, specifically contemplated embodiment that should be considered disclosed unless the context specifically indicates otherwise. It will be further understood that the endpoints of each of the ranges are significant both in relation to the other endpoint, and independently of the other endpoint unless the context specifically indicates otherwise. Finally, it should be understood that all of the individual values and sub-ranges of values contained within an explicitly disclosed range are also specifically contemplated and should be considered disclosed unless the context specifically indicates otherwise. The foregoing applies regardless of whether in particular cases some or all of these embodiments are explicitly disclosed. 
     Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of skill in the art to which the disclosed method and compositions belong. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present method and compositions, the particularly useful methods, devices, and materials are as described. Publications cited herein and the material for which they are cited are hereby specifically incorporated by reference. Nothing herein is to be construed as an admission that the present invention is not entitled to antedate such disclosure by virtue of prior invention. No admission is made that any reference constitutes prior art. The discussion of references states what their authors assert, and applicants reserve the right to challenge the accuracy and pertinency of the cited documents. It will be clearly understood that, although a number of publications are referred to herein, such reference does not constitute an admission that any of these documents forms part of the common general knowledge in the art. 
     Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.