Patent Publication Number: US-2021162348-A1

Title: Membrane structure body having  matrix structure and biomolecule filter using same

Description:
FIELD OF THE DISCLOSURE 
     The present disclosure relates to a membrane structure having a biomolecule filter and a biomolecule filter using the same, and more specifically, to a membrane structure having a plurality of window cells arranged in a matrix shape and a biomolecule filter for filtering biomolecules included in a sample using the same. 
     RELATED ART 
     The present disclosure claims priority to Korean Patent Application No. 10-2018-0010006 filed on Jan. 26, 2018, and the entire specification is a reference of the present disclosure. 
     In general, a biomolecule refers to a substance constituting a living body (e.g., nucleic acids, proteins, microvesicles, etc.) or a substance derived from a living body. Recently, as it is known that diseases such as cancer and Alzheimer&#39;s disease can be diagnosed using exosomes (i.e., signaling substances between cells), interests and studies are rapidly increasing with respect to technologies for efficient separation of specific biomolecules (e.g., exosomes). 
     However, the existing technology for separating vesicles in body fluids by fixing antibodies, which bind to vesicle proteins to microchip, requires that a centrifugation process be performed as a pretreatment process and that expensive equipment be used for the fixation of antibodies. Therefore, there were problems in that biomolecules may be damaged and much time and cost are required for the separation of biomolecules. 
     Additionally, as disclosed in Korean Patent Application Publication No. 10-0550515, the existing technology for separating biomolecules using a porous membrane employs a simple film type dry film resist (DFR) film or polycarbonate film, there is a problem in that the porous membrane has poor durability and it is difficult to handle and install the porous membrane. Moreover, according to the existing technology, biomaterials are filtered in a static state where a sample is filled into channel on which a porous membrane is installed, there is a problem in that a porous membrane is blocked by other substances included in the sample, thus rapidly deteriorating its filtering efficiency. 
     SUMMARY OF THE DISCLOSURE 
     A technical problem to solve in the present disclosure is to provide a membrane structure having a matrix structure, which in the process of separating biomolecules included in a sample, not only prevents damage to a biomolecule and saves time and money, but also improves the durability of a membrane structure and prevents blockage of the membrane structure by substances other than biomolecules to be filtered and subsequent deterioration in its filtering efficiency; and a biomolecule filter using the same. 
     A membrane structure having a matrix structure according to an embodiment of the present disclosure includes a filtering part, which includes a window region in which a plurality of window cells are formed in a matrix shape; and a blocking region in which the window cells are not formed, and which filters biomolecules from a sample moving along the window region; and a support part, which extends from the filtering part so as to support the filtering part, wherein each of the window cells formed in the window region of the filtering part is configured to have micro-holes allowing the biomolecules having a predetermined size or less to pass therethrough, and thus filters biomolecules included in the sample. 
     In an embodiment, the filtering part may include a plurality of window regions, and the plurality of window regions be configured to be placed side by side by being spaced apart one after the other with the blocking region placed therebetween. 
     In an embodiment, the membrane structure the membrane structure may have a matrix structure with a laminated structure, which includes a substrate on which through holes constituting the window cells are formed; and a porous membrane which is laminated on the substrate and covers one side opening of the through holes. 
     In an embodiment, the substrate may include a silicon substrate. 
     In an embodiment, the substrate may further include a silicon oxide (SiO 2 ) layer laminated on the silicon substrate. 
     In an embodiment, the porous membrane may consist of silicon nitride (Si 3 N 4 ). 
     A biomolecule filter according to an embodiment of the present disclosure is a filter for filtering biomolecules included in a sample using the membrane structure according to any of the embodiments described above, which includes a first housing, which is located on a side of one surface of the membrane structure and receives a sample and transports the sample along the window region of the membrane structure; and a second housing, which is located on a side of the other surface of the membrane structure and collects biomolecules coming out through the other surface of the membrane structure. 
     In an embodiment, the first housing may include an inlet, through which the sample is flowed in; a first outlet, which discharges a remaining sample from which at least some of the biomolecules are separated; and a first flow path, which transports the sample flowed in through the inlet along the window region of the membrane structure and then delivers the sample to the first outlet. 
     In an embodiment, the first flow path may include a flow path groove, which is formed along the window region of the membrane structure and connects the inlet and the first outlet; and a diaphragm, which is formed around the flow path groove and prevents leakage of a sample. 
     In an embodiment, the first housing may further include a first adhesive part to which an adhesive is applied, which is adhered to a support part of the membrane structure; and a first vent hole, which allows the first adhesive part to communicate with the outside of the first housing. 
     In an embodiment, the second housing may include a second outlet, which discharges biomolecules separated through the membrane structure; and a second flow path, which transports biomolecules coming out through the other surface of the membrane structure and delivers the biomolecules to the second outlet. 
     In an embodiment, the second flow path may include a second flow path groove, which includes a plurality of guide grooves that are configured to extend side by side at mutually spaced intervals to guide the movement of the biomolecules coming out from the other surface of the membrane structure in a certain direction; a collecting groove, which collects the biomolecules guided and moved by the plurality of guide grooves and delivers the biomolecules to the second outlet; and a second diaphragm, which is formed around the second flow path groove and prevents leakage of biomolecules. 
     In an embodiment, the second housing may further include a second adhesive part to which an adhesive is applied, which is adhered to a support part of the membrane structure; and a second vent hole, which allows the second adhesive part to communicate with the outside of the second housing. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a front perspective view showing a biomolecule filter according to an embodiment of the present disclosure. 
         FIG. 2  is a rear perspective view showing the biomolecule filter shown in  FIG. 1 . 
         FIG. 3  is an exploded perspective view showing the biomolecule filter shown in  FIG. 1 . 
         FIG. 4  is a plan view showing a membrane structure having a matrix structure according to an embodiment of the present disclosure. 
         FIG. 5  is an enlarged view showing a window cell portion of the membrane structure shown in  FIG. 4 . 
         FIG. 6  is a vertical cross-sectional view showing part A-A′ shown in  FIG. 5 . 
         FIG. 7  is a perspective view showing the inner surface of the first housing shown in  FIG. 3 . 
         FIG. 8  is a perspective view showing the inner surface of the second housing shown in  FIG. 3   
     
    
    
     DESCRIPTION OF EXEMPLARY EMBODIMENTS 
     Hereinafter, embodiments of the present disclosure will be described in detail with reference to the accompanying drawings in order to clarify solutions to the technical problems of the present disclosure. However, in explaining the present disclosure, a description of related publicly known technology will be omitted if it makes the gist of the present disclosure unclear. In addition, the terms to be described later are terms defined in consideration of functions in the present disclosure, which may vary according to intentions or practices of a designer, a manufacturer, etc. Therefore, the definition should be made based on the contents throughout this specification. 
     In the present specification, the term “biomolecule” refers to not only a substance constituting a living body (nucleic acids, proteins, microvesicles, etc.) and a substance derived from a living body, but also all the substances formed by binding, decomposition, modification, or mutation of these substances. 
       FIG. 1  shows the biomolecule filter  100  according to an embodiment of the present disclosure is shown in a front perspective view. 
     As shown in  FIG. 1 , the biomolecule filter  100  according to an embodiment of the present disclosure may include a first housing  110 , a second housing  120 , and a membrane structure (not shown) installed between the first housing  110  and the second housing  120 . 
     The first housing  110  is located on a side of one surface of a membrane structure and is configured to receive a sample and bring the sample into contact with the membrane structure. For this purpose, the first housing  110  may include a first outlet  111 , an inlet  112 , and a first flow path (not shown). 
     The inlet  112  is configured such that a sample being supplied from the outside of the first housing  110  flows into the inside of the first housing  110 . For example, the inlet  112  may be configured to include a protrusion for inserting a sample supply pipe formed on an outer surface of the first housing  110 , and a through hole extending from an end of the protrusion to an inner surface of the first housing  110 . 
     The first outlet  111  is configured to discharge a remaining sample, in which at least some the biomolecules are separated from the sample flowed into the inlet  112 . For example, the first outlet  111  may be configured to include a protrusion for inserting a sample collection tube formed on the outer surface of a first housing  110  and a through hole extending from the inner surface of the first housing  110  to the distal end of the sample collection tube. 
     As will be described again below, a first flow path of the first housing  110  transports the sample flowed in through the inlet  112  along a certain pathway on one surface thereof while bringing the sample into contact with one surface of the membrane structure, and delivers the sample to the first outlet  111 . 
     Additionally, the first housing  110  may further include a first vent hole  116  which allows the inside of the first housing  110  to communicate with the outside of the first housing  110 . 
       FIG. 2  shows the biomolecule filter  100  shown in  FIG. 1  in a rear perspective view. 
     As shown in  FIG. 2 , the second housing  120  of the biomolecule filter  100  according to an embodiment of the present disclosure is located on a side of the other surface of the membrane structure corresponding to a first housing  110  and is configured to collect and discharge biomolecules coming out through the other surface of the membrane structure. For this purpose, the second housing  120  may include a second outlet  121  and a second flow path (not shown). 
     The second outlet  121  is configured to discharge biomolecules separated through the membrane structure. For example, the second outlet  121  may be configured to include a protrusion for inserting biomolecule collection tube formed on an outer surface of the second housing  120  and a through hole extending from an inner surface of the second housing  120  to a distal end of the protrusion for inserting the biomolecule collection tube. 
     As will be described again below, the second flow path of the second housing  120  is configured to collect biomolecules coming out through the other surface of the membrane structure and deliver the biomolecules to the second outlet. 
     Additionally, the second housing  120  may further include a second vent hole  126  which allows the inside of the second housing  120  to communicate with the outside of the second housing  120 . 
       FIG. 3  shows the biomolecule filter  100  shown in  FIG. 1  in an exploded perspective view. 
     As shown in  FIG. 3 , the biomolecule filter  100  includes a membrane structure  130  and filters biomolecules included in a sample using the membrane structure  130 . That is, the first housing  110  is located on a side of one surface of the membrane structure  130  and is coupled to one surface of the membrane structure  130 , and the second housing  120  is located on a side of the other surface of the membrane structure  130  and is coupled to the other surface of the membrane structure  130 . According to an embodiment, the first housing  110  and the second housing  120  may be configured to accommodate the membrane structure  130 . For example, the first housing  110  and the second housing  120  may accommodate the membrane structure  130  in the inner space formed by a mutual binding, and as described above, the first housing  110  may be coupled to one surface of the membrane structure  130 , and the second housing  120  may be coupled to the other surface of the membrane structure  130 . 
       FIG. 4  shows the membrane structure  130  having a matrix structure according to an embodiment of the present disclosure in a plan view. 
     As shown in  FIG. 4 , the membrane structure  130  according to an embodiment of the present disclosure is a membrane-shaped structure configured to filter biomolecules included in a sample, and it includes a filtering part  132  and a support part  134 . 
     The filtering part  132  is a part in which filtering of biomolecules is performed, and it includes a window region (Dw), where the window cells (w) are formed, and a blocking region (Db) where the window cells (w) are not formed. 
     The window region (Dw) of the filtering part  132  is a region in which a plurality of window cells (w) are configured to be formed in a matrix form along the moving path of a sample so as to contact the sample. The first housing  110  being coupled to the membrane structure  130  forms a flow path along the window region (Dw) of the filtering part  132  and transports the sample through the corresponding flow path. The filtering part  132 , as described above, filters biomolecules from the sample moving along the window region (Dw). For this purpose, each of the window cells (w) formed in the window region (Dw) has a plurality of micro-holes allowing the biomolecules having a predetermined size or less to pass therethrough. 
     The blocking region (Db) of the filtering part  132  is a region excluding the window region (Dw) among the entire regions of the filtering part  132 , where the filtering of biomolecules does not occur. In the blocking region (Db), a diaphragm of the first housing  110 , which will be described later, is located, thereby preventing leakage of a sample moving along the window region (Dw). 
     According to an embodiment, the filtering part  132  may include a plurality of window regions (Dw) as shown in  FIG. 4 . In this case, the plurality of window regions (Dw) may be arranged side by side with the blocking region (Db) placed therebetween. 
     Meanwhile, the support part  134  is configured to extended or enlarged from the filtering part  132  so as to support the filtering part  132 . That is, the support part  134  is a part corresponding to a frame that supports a filtering part  132 , by being gripped by a user when transporting the membrane structure  100  or being adhered or inserted into another structure when the membrane structure  100  is installed. 
     When a membrane structure  130  is configured in the form of a square plate as shown in  FIG. 4 , the filtering part  132  may be located at the central portion of the membrane structure  130 , and the support part  134  may be located at the edge portion of the membrane structure  130 . The shape and size of the membrane structure  130 , the locations of the filtering part  132  and the support part  134 , etc. can be variously changed according to the environment to which the membrane structure  100  is applied. 
     One thing to be noted in the present disclosure is that the biomolecules included in the sample to be filtered are not separated by window cells (w) formed in the window region (Dw), but they are separated by micro-holes in each window cell (w). That is, the size and shape of the window cell are independent of the size of the biomolecules to be filtered. 
       FIG. 5  shows an enlarged view of the window cell (w) portion of the membrane structure  130  shown in  FIG. 4   
     As shown in  FIG. 5 , a plurality of fine holes (h) are formed in each window cell (w) formed in the filtering part  132  of the membrane structure  130 . The size of the window cell (w) may be configured, for example, as a 1.2 mm×1.2 mm size when the membrane structure  130  is composed of a 50 mm×50 mm size. That is, the size and shape of the window cell (w) are independent of the size of biomolecules to be filtered, and can be variously changed according to the strength, thickness, etc. of the window cell structure. 
     Meanwhile, the size of the micro-holes (h) formed in the window cell (w) is determined according to the size of biomolecules to be filtered. That is, the micro-holes (h) formed in the window cell (w) may be configured to have a diameter corresponding to a range between 10 nm or more and 300 nm or less. For example, when the biomolecules to be filtered are exosomes, a plurality of micro-holes (h) formed in the window cell (w) may be configured to have a diameter corresponding to a range between 10 nm or more and 300 nm or less. 
       FIG. 6  shows a part A-A′ shown in  FIG. 5  in a vertical sectional view. 
     As shown in  FIG. 6 , the membrane structure  130  may have a laminated structure including a substrate  130   a  and a porous membrane  130   b.    
     The substrate  130   a  corresponds to the basic frame of the membrane structure  130  and it may include a silicon substrate L 1  that establishes a core layer. Additionally, the substrate  130   a  may further include a silicon oxide layer (L 2 ) laminated on the silicon substrate L 1  to prevent deformation or cracking of the substrate due to residual stress. The silicon oxide layer L 2  may be laminated on both the upper and lower surfaces of the silicon substrate L 1  as shown in  FIG. 6 , and it may be laminated on only one of the upper and lower surfaces according to an embodiment. In an embodiment, the silicon oxide layer L 2  may be laminated on the silicon a substrate L 1  through a deposition process of a semiconductor manufacturing technology. 
     Through holes (Hw) for forming the window cell (w) are formed in such a substrate  130   a . In this case, the through holes (Hw) may be formed through a lithography process of a semiconductor manufacturing technology. 
     The porous membrane  130   b , which corresponds to the porous structure of the window cell (w), is supported by being laminated on one surface of the substrate  130   a  with a plurality of micro-holes (h) passing biomolecules of a certain size or less, and it covers one side opening of the through hole (Hw) 
     In an embodiment, the porous membrane  130   b  may be composed of a silicon-based compound. For example, the porous membrane ( 130   b ) may be composed of at least one compound selected from the group consisting of silicon nitride (Si 3 N 4 ), silicon oxide (SiO 2), and silicon carbide (SiC), and in particular silicon nitride (Si 3 N 4 ). 
     Silicon nitride is a material mainly used as a passivation film to prevent alkali ions from diffusing into the semiconductor surface during the manufacture of semiconductors. In the present disclosure, considering the characteristics of silicon nitride (i.e., high strength (bending strength of 100-140 kg/mm 2  at room temperature), low thermal expansion rate (thermal expansion rate: 3×10 −6 /° C.), and excellent heat shock resistance), silicon nitride is selected as a substance for the porous membrane  130   b.    
     In this case, the porous membrane  130   b  may be configured to have a nano-sized thickness, that is, a thickness corresponding to a range between 50 nm or more and 500 nm or less. When the thickness of the porous membrane  130   b  is less than 50 nm, the strength of the porous membrane  130   b  decreases and is likely to be broken during the process of manufacture or use. In contrast, when the thickness of the porous membrane  130   b  is greater than 500 nm, the time required for the separation of biomolecules becomes longer and the separation efficiency decreases. In order to simultaneously satisfy the membrane strength and the separation efficiency of biomolecules, the porous membrane  130   b  may be configured to have a thickness corresponding to a range between 100 nm or more and 300 nm or less. 
     Meanwhile, the size of the micro-holes (h) formed in the porous membrane  130   b  is determined according to the size of the biomolecules to be filtered. That is, the plurality of the micro-holes (h) formed in the porous membrane  130   b  may be configured to have a diameter corresponding to a range between 10 nm or more and 3,000 nm or less. For example, when the biomolecules to be filtered are exosomes, a plurality of micro-holes (h) formed in the porous membrane  130   b  may be configured to have a diameter corresponding to a range between 10 nm or more and 300 nm or less. In this case, the nano-sized micro-holes formed on the porous membrane  130   b  may be formed through electron beam lithography or X-ray lithography process with high resolution. 
       FIG. 7  shows the inner surface of the first housing  110  shown in  FIG. 3  in a perspective view. 
     As shown in  FIG. 7 , the first housing  110  is located on a side of one surface of the membrane structure  130  and is configured to receive a sample including biomolecules to be filtered and transports the sample along the window region (Dw) of the membrane structure  130 . For this purpose, the first housing  110  includes a first outlet  111 , an inlet  112 , and a first flow path  113 , and according to an embodiment, it may further include a first vent hole  116 , a first diaphragm  117 , etc. 
     As mentioned above, the inlet  112  is configured such that the sample including biomolecules to be filtered flows in from the outside of the first housing  110  to the inside. Additionally, the first outlet  111  is configured to discharge a remaining sample, in which at least some of the biomolecules are separated from the sample flowed in through the inlet  112 . 
     The first flow path  113 , which connects the inlet  112  and the first outlet  111 , is configured to transport the sample flowed in through the inlet  112  along the window region (Dw) while bringing the sample into contact with the window region (Dw) of the membrane structure  130 , and then delivers the sample to the first outlet  111 . In this case, the first flow path  113  may be configured to deliver the entire sample flowed therein to the first outlet  111  after bringing the sample into contact with a plurality of window regions (Dw) of the membrane structure  130 . 
     For example, the first flow path  113  may be configured to form, on one surface of the membrane structure  130 , a moving path of the sample in the form of a meander that connects the inlet  112  and the first outlet  111 . In this case, the first flow path  113  be configured such that it transports the sample flowed in through the inlet  112  along the window region of the membrane structure  130  in a zigzag direction as shown in  FIG. 7  and gradually advances the sample to a side of the first outlet  111 . 
     Additionally, the first flow path  113  may include the first flow path groove  113   a  and the first diaphragm  113   b . The first flow path groove  113   a  is a groove structure connecting the inlet  112  and the first outlet  111 , and it may be configured in a meander form as described above. The first diaphragm  113   b  is formed around the first flow path groove  113   a  and it may be configured to prevent leakage of a sample from the first flow path groove  113   a.    
     In an embodiment, the first housing  110  may further include a sealing member (not shown) that seals between one surface of the membrane structure  130  and the first diaphragm  113   b  of the first flow path  113 . Such a sealing member may be composed of a polymer synthetic resin having adhesiveness and water resistance. According to an embodiment, the sealing member may include a rubber member which comes in close contact with one surface of the membrane structure  130 . In this case, the first diaphragm  113   b  may include an installation groove  113   c  into which the rubber member is inserted to be installed. 
     Additionally, in an embodiment, the first diaphragm  113   b  may further include a first adhesive blocking groove  113   d  which is formed between the first flow path groove  113   a  and the first adhesive part  114  described below. The first adhesive blocking groove  113   d  is configured to receive the remaining amount of the adhesive flowing out of the corresponding adhesive surface during an adhesion between the first adhesive part  114  and the membrane structure  130 , so that the adhesive can be prevented from flowing into the first flow path groove  113   a.    
     Meanwhile, the first adhesive part  114  is a part to which an adhesive is applied, and it is adhered to one surface of the membrane structure  130 . In this case, the first adhesive part  114  has a height difference with the first diaphragm  113   b  of the first flow path  113  in consideration of the thickness of the adhesive layer being formed by the adhesive and it may be formed to be lower than the first diaphragm  113   b . Additionally, the first adhesive part  114  may be configured as an annular structure to be adhered to the support part  134  corresponding to the edge portion of the membrane structure  130 . 
     The first adhesive receiving groove  115  is a groove formed around the outer periphery of the first adhesive part  114 , and basically, an adhesive may be applied as in the first adhesive part  114 . In this case, the first adhesive receiving groove  115  may be configured to receive a remaining amount of the adhesive flowing out of the corresponding adhesive surface during an adhesion between the first adhesive part  114  and the membrane structure  130  and to be adhered to the membrane structure  130 . When the first adhesive part  114  is configured in an annular structure, the first adhesive receiving groove  115  may be configured to be formed as an annular groove formed along the first adhesive part  114 . 
     The first vent hole  116  is configured to allow the first adhesive part  114  to communicate with the outside of the first housing  110 . The first vent hole  116  can discharge the gas generated during an adhesion between the first adhesive part  114  and the membrane structure  130  or a remaining amount of adhesive thereof to the outside. In this case, the first vent hole  116  may be formed in the first adhesive receiving groove  115 . 
     The first diaphragm  117 , which is the outermost structure of the first housing  110 , is configured to protect the membrane structure  130  by preventing external exposure of the membrane structure  130 . For example, the first diaphragm  117  may be configured to adhere to the outermost edge portion of the membrane structure  130 . In this case, the first diaphragm  117  may be adhered to the membrane structure  130  by the adhesive being flowed therein in the process of the adhesion of the first adhesive part  114  and the first adhesive receiving groove  115  with the membrane structure  130 , even when an adhesive is not applied in advance as is the case with the first adhesive part  114  or the first adhesive receiving groove  115 . 
       FIG. 8  shows the inner surface of the second housing  120  shown in  FIG. 3  in a perspective view. 
     As shown in  FIG. 8 , the second housing  120  is located on a side of the other surface of the membrane structure  130  corresponding to the first housing  110  located on a side of one surface of the membrane structure  130 , and is configured to collect and discharge biomolecules coming out through the other side of the membrane structure  130 . For this purpose, the second housing  120  includes the second outlet  121  and the second flow path  123 , and according to an embodiment, may further include a second adhesive part  124 , a second adhesive receiving groove  125 , a second vent hole  126 , a second diaphragm  127 , etc. 
     As mentioned above, the second outlet  121  is configured to discharge the biomolecules separated through the membrane structure  130 . 
     The second flow path  123  is configured to transport the biomolecules coming out through the micro-holes of each window cell (w) on the other surface of the membrane structure  130  and deliver them to the second outlet  121 . For this purpose, the second flow path  123  may include the second flow path grooves ( 123   a  and  123   b ) and a second diaphragm  123   c.    
     The second flow path grooves ( 123   a ,  123   b ) are of groove structures which connect the other surface of the membrane structure  130  and the second outlet  121 , and they may include a plurality of guide grooves  123   a  and collection grooves  123   b.    
     The plurality of guide grooves  123   a  are grooves for guiding the movement of the biomolecules coming out through each window cell (w) from the other surface of the membrane structure  130  in a certain direction (i.e., in a direction of the collection groove  123   b ), and they may be composed of elongated grooves extending side by side at regular intervals so as to cover at least the region corresponding to the filtering part  132  of the entire region of the membrane structure  130 . 
     The collection groove  123   b  is a groove which is guided by the plurality of guide grooves  123   a  and collects and delivers biomolecules to the second outlet  121 , and the collection groove  123   b  may be configured to connect each guide groove  123   a  with the second outlet  121 . 
     The second diaphragm  123   c  may be configured to be may be formed around the second flow path grooves ( 123   a ,  123   b ) so as to prevent leakage of biomolecules. 
     In an embodiment, the second housing  120  may further include a sealing member (not shown) for sealing between the other surface of the membrane structure  130  and the second diaphragm  123   c . Such a sealing member may be composed of a polymer synthetic resin having adhesiveness and water resistance. 
     Additionally, in an embodiment, the second diaphragm  123   c  may further include a second adhesive blocking groove  123   d  formed between the second flow path grooves ( 123   a ,  123   b ) and the second adhesive part  124  to be described later. The second adhesive blocking groove  123   d  may be configured to receive the remaining amount of the adhesive flowing out of the corresponding adhesive surface during an adhesion between the second adhesive part  124  and the membrane structure  130 , and thereby block the adhesive from flowing into the second flow path grooves ( 123   a ,  123   b ). 
     Meanwhile, the second adhesive part  124  is a portion to which an adhesive is applied, and it is adhered to the other surface of the membrane structure  130 . In this case, the second adhesive part  124  has a height difference with the second diaphragm  123   c  of the second flow path  123  in consideration of the thickness of the adhesive layer being formed by the adhesive and it may be formed to be lower than the second diaphragm  123   c . Additionally, the second adhesive part  124  may be configured in an annular structure to be adhered to the support part  134  corresponding to the edge portion of the membrane structure  130 . 
     The second adhesive receiving groove  125  is a groove formed around the outer periphery of the second adhesive part  124 , and basically, an adhesive may be applied as in the second adhesive part  124 . In this case, the second adhesive receiving groove  125  may be configured to receive a remaining amount of the adhesive flowing out of the corresponding adhesive surface during an adhesion between the second adhesive part  124  and the membrane structure  130  and to be adhered to the membrane structure  130 . When the second adhesive part  124  is configured in an annular structure, the second adhesive receiving groove  125  may be configured to be formed as an annular groove formed along the second adhesive part  124 . 
     The second vent hole  126  is configured to allow the second adhesive part  124  to communicate with the outside of the second housing  120 . The second vent hole  126  can discharge the gas generated during an adhesion between the second adhesive part  124  and the membrane structure  130  or a remaining amount of adhesive thereof to the outside. In this case, the second vent hole  126  may be formed in the second adhesive receiving groove  125 . 
     The second diaphragm  127 , which is the outermost structure of the second housing  120 , is configured to protect the membrane structure  130  by preventing external exposure of the membrane structure  130 . For this purpose, the second diaphragm  127  may be configured to adhere to the outermost edge portion of the membrane structure  130 . In this case, the second diaphragm  127  may be adhered to the membrane structure  130  by the adhesive being flowed therein in the process of the adhesion of the second adhesive part  124  and the second adhesive receiving groove  125  with the membrane structure  130 , even when an adhesive is not applied in advance as is the case with the second adhesive part  124  or the second adhesive receiving groove  125 . 
     The biomolecule filter  100  configured as such can discharge the remaining sample, in which some of the biomolecules are separated, to the first outlet  111  of the first housing  110 , while discharging the filtered biomolecules to the second outlet  121  of the second housing  120 , after filtering the biomolecules included in the sample supplied through the inlet  112  of the first housing  110  while transporting the sample along the window region (Dw) of the membrane structure  130 . Additionally, the biomolecule filter  100  can filter the biomolecules included in the sample by repeatedly cycling a certain amount of the sample along the window region (Dw) of the membrane structure  130  in such a manner that the remaining sample discharged through the first outlet  111  is received again through the inlet  112 . 
     As described above, according to the present disclosure, it is possible to prevent damage to biomolecules and reduce the time and cost required for a biomolecule separation process by separating the biomolecules included in the sample via filtration through a membrane structure with a porous structure. 
     In particular, it is possible to improve the durability of a membrane structure and facilitates easy handling and installation of the membrane structure by forming a plurality of window cells on the membrane structure and by constructing the fragile porous structure via divisions into each window cell unit. 
     Additionally, as window cells filter the biomolecules included in a sample while transporting the sample along the window region of a membrane structure arranged in a matrix form, the blockage of the membrane structure caused by substances other than the biomolecules to be filtered and the subsequent deterioration in filtering efficiency can be prevented. 
     Additionally, the yield of biomolecules can be improved while reducing the amount of a sample required for acquiring the biomolecules, by repeatedly cycling a certain amount of the sample along the window region of a membrane structure and filtering the biomolecules contained in the sample. 
     Further, the embodiments according to the present disclosure can solve various technical problems other than those mentioned in the present specification in related technical fields as well as in the present technical field. 
     Thus far, the present disclosure has been described with reference to specific embodiments. However, those skilled in the art will clearly understand that various modified embodiments can be implemented in the technical scope of the present disclosure. Therefore, the embodiments disclosed above should be considered from an explanatory point of view rather than a limited point of view. That is, the scope of the true technical idea of the present disclosure is indicated in the claims, and all the differences within the scope of the present disclosure should be interpreted as being included in the present disclosure. 
     Effects of the Invention 
     According to the present disclosure, it is possible to prevent damage to biomolecules and reduce the time and cost required for a biomolecule separation process by separating the biomolecules included in the sample via filtration through a membrane structure with a porous structure. 
     In particular, it is possible to improve the durability of a membrane structure and facilitates easy handling and installation of the membrane structure by forming a plurality of window cells on the membrane structure and by constructing the fragile porous structure via divisions into each window cell unit. 
     Additionally, as window cells filter the biomolecules included in a sample while transporting the sample along the window region of a membrane structure arranged in a matrix form, the blockage of the membrane structure caused by substances other than the biomolecules to be filtered and the subsequent deterioration in filtering efficiency can be prevented. 
     Additionally, the yield of biomolecules can be improved while reducing the amount of a sample required for acquiring the biomolecules, by repeatedly cycling a certain amount of the sample along the window region of a membrane structure and filtering the biomolecules contained in the sample. 
     Further, any one with ordinary skill in the art to which the present disclosure belongs will clearly understand from the following description that various embodiments according to the present disclosure can solve various technical problems not mentioned above.
           100  BIOMOLECULE FILTER  110 : A FIRST HOUSING     111  The first outlet has a first outlet ( 112 ) and an outlet ( 112 )     113  FIRST FLOW PATH  114 : A FIRST ADHESIVE PART     115  FIRST ADHESIVE RECEIVING GROOVE  116 : FIRST VENT HOLE     117  OUTER WALL PART  120 : SECOND HOUSING     121  SECOND OUTLET  123 : SECOND FLOW PATH     124  The second adhesive part  125 : the second adhesive receiving groove     126  The second vent hole  127 : the second outer wall part     130  MEMBRANE STRUCTURE  132 : A FILTERING UNIT     134  SUPPORT