Patent Publication Number: US-2020278528-A1

Title: Holding a substrate within a secondary device

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application claims the benefit of Provisional Application No. 62/812,695, filed Mar. 1, 2019. 
    
    
     INCORPORATION BY REFERENCE 
     All publications and patent applications mentioned in this specification are incorporated herein by reference in their entirety to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. 
     FIELD 
     This disclosure relates generally to an apparatus for holding a substrate and, in particular, to an apparatus for securely holding a microscope slide on or within an imaging device. 
     BACKGROUND 
     When a substrate is not consistently secured in a repeatable, predictable manner regardless of substrate inconsistencies or deformities, the images may vary from substrate to substrate and it may be difficult to determine proper surface locations. As a result, practitioners, researchers, and those imaging samples on substrates continue to seek an apparatus for consistently, repeatably, and predictably securing substrates of varying inconsistencies or deformities. 
     SUMMARY OF THE DISCLOSURE 
     In a first aspect, a device is provided. The device comprises a frame comprising a first arm, a second arm, and at least two securing blocks; and a secure bar comprising at least two securing blocks, wherein the at least two securing blocks of the frame and the at least two securing blocks of the secure bar are on opposing sides of a cavity, wherein (1) one end of the secure bar is adjustable relative to the other end of the secure bar, or (2) one end of the second arm is adjustable relative to the other end of the second arm, wherein at least one of the securing blocks is at the adjustable end of the secure bar or at the adjustable end of the second arm, and wherein the adjustable end of the secure bar or the adjustable end of the second arm is adjustable along essentially a z-axis. 
     In some embodiments, the devices further comprises a flexure to permit adjustment of the adjustable end of the secure bar or the adjustable end of the second arm. The flexure can be at least one of a cut-out, a track, a groove, a notch, or one or more flexible materials. In some embodiments, the flexure comprises a clasp configured to adjust at least one of the position, angle, or location of the adjustable end of the secure bar or the adjustable end of the second arm. Each of the securing blocks can comprise a stopper and a ramp. In some embodiments, the secure bar is moveable relative to the frame. The secure bar can be connected to the first arm of the frame with at least one bearing, the bearing configured to permit the secure bar to move relative to the frame. The device can comprise a mount, wherein the mount connects the device to a secondary device. In some embodiments, the secondary device is a scanner, an imaging microscope, or a fluorescence microscope. The device can comprise a slider adjoined to the secure bar and a connector adjoined to the mount, wherein a portion of the slider fits within a track of the connector. In some embodiments, the device comprises a motor to move the secure bar relative to the frame. The first arm can comprise a platform configured to support a substrate. In some embodiments, the second arm comprises a pedestal configured to support a substrate. 
     In another aspect, a device is provided. The devices comprises a frame comprising a first arm and a second arm and at least two securing blocks; a secure bar comprising at least two securing blocks; and a flexure positioned between the at least two securing blocks of the first arm or between the at least two securing blocks of the secure bar, wherein the secure bar is movable relative to the frame, and wherein the at least two securing blocks of the frame and the at least two securing blocks of the secure bar are on opposing sides of a cavity. 
     In some embodiments, the flexure is positioned between the at least two securing blocks of the secure bar. The flexure can be configured to adjust at least one of the position, angle, or location of at least one of the at least two securing blocks of the secure bar or at least one of the at least two securing blocks of the first arm. In some embodiments, the flexure is configured to adjust at least one of the position, angle, or location of one of the at least two securing blocks of the secure bar independently of the other one of the at least two securing blocks of the secure bar. The flexure can be configured to adjust at least one of the position, angle, or location of one of the at least two securing blocks of the secure bar independently of the other one of the at least two securing blocks of the secure bar and independently of the at least two securing blocks of the frame. The flexure can comprise a clasp. In some embodiments, the flexure is at least one of one or more flexible materials, a cut-out, a track, a groove, or a notch. The secure bar can be movable to an open position to permit the insertion of the substrate, and movable to a closed position to permit the holding of the substrate. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIGS. 1A-1B  show an example securing block. 
         FIGS. 2A-2D  show an example secure bar. 
         FIGS. 3A-3B  show an example secure bar. 
         FIGS. 4A-4C  show an example substrate holder. 
         FIGS. 5A-5C  show an example substrate holder. 
         FIGS. 6A-6D  show an example substrate holder. 
         FIGS. 7A-7B  show an example substrate holder attached to a mount. 
     
    
    
     DETAILED DESCRIPTION 
     This disclosure is directed to an apparatus for securely holding a substrate, such as a microscope slide. Suitable devices, systems, and/or methods for holding a substrate may include those described in one or more of the following U.S. patents and published applications, each of which is hereby incorporated by reference in its entirety: U.S. Pat. No. 9,857,580; 2017/0315340; 2017/0363850; 2017/0322407; 2017/0261735; 2018/0003939; 2018/0003940. 
     Securing Block 
       FIGS. 1A-1B  show a securing block  100 . The securing block  100  includes a main body  102  with a stopper  104  and a ramp  106 , both of which extend from the same side of the main body  102 . The securing block  100  can be configured and/or oriented to cause a substrate to travel vertically, downwardly, horizontally, orthogonally, or combinations thereof. Therefore, the securing block  100  can be inverted, rotated, or adjusted in any desired manner to cause the substrate to travel in at least one desired direction. In one embodiment, the securing block  100  provides a controlled references surface for a substrate during imaging, processing, holding, or the like. In one embodiment, the securing block  100  provides a flat substrate surface during imaging, processing, holding, or the like when a substrate has a non-flat (e.g., curved, twisted, warped, or the like) surface. 
     In one embodiment, the main body  102  can be a single piece. In one embodiment, the main body  102  can be multiple pieces, including at least two pieces. When the main body  102  is at least two pieces, a first piece includes the stopper  104  and a second piece includes the ramp  106 . 
     In one embodiment, the main body  102  includes at least one hole (not shown) to accept at least one fastener  108  for attachment to a frame or a secure bar. In one embodiment, the securing block  100  includes at least one height adjuster (not shown), such as a shim, to adjust the height of the securing block  100  relative to the frame or the secure bar. 
     In one embodiment, the stopper  104  can be any appropriate shape, including, but not limited to, triangular, hemispherical, parabolic, trapezoidal, rectangular, cubical, or the like. In one embodiment, the ramp  106  can be any appropriate shape, including, but not limited to, triangular, hemispherical, parabolic, trapezoidal, or the like. 
     Secure Bar 
       FIGS. 2A-2D  show a secure bar  200 . The secure bar  200  includes a bracket  202  and a strut  204 . The strut  204  includes a proximal end including a first securing block  100  and a distal end  212  including a second securing block  100 . In one embodiment, the bracket  202  and the strut  204  are a single piece. In one embodiment, the bracket  202  and the strut  204  are separate pieces. When the bracket  202  and the strut  204  are separate pieces, the bracket  202  and the strut  204  can be attached, adjoined, or adhered to each other by screws, dowels, rods, rivets, at least one dovetail joint, at least one tongue-and-groove joint, an adhesive, an epoxy, at least one magnet, or by any other appropriate manner in which to attach, adjoin, or adhere two or more pieces. 
     In one embodiment, the bracket  202  can include at least one bearing  208  to permit rotation of the bracket  202  relative to a substrate (not shown) or at least one other component, including, without limitation, the strut  204  or a frame (not shown). In one embodiment, the bracket  202  can include two or more bearings  208 . 
     In one embodiment, the bracket  202  includes a projection  206  extending outwardly from the bracket  202 . In one embodiment, the bracket  202  includes two projections  206  extending outwardly, in the same direction, from the bracket  202 . In one embodiment, the bracket  202  includes a flange  214  extending outwardly from the bracket  202  in the same direction as the projection  206 . 
     In one embodiment, the secure bar  200  can include at least one flexure  216 , such as one or more flexible materials, a cut-out (e.g., a track, a groove, a notch, or the like), a material less stiff than the other material composing the strut  204 , or the like. The at least one flexure  216  allows for movement of the securing block  100  via the proximal end or the distal end  212  of the secure bar  200  to move along a given axis (for example, the z-axis) relative to the other end of the secure bar  200 . The at least one flexure  216  can extend at least partially through a top face of the strut  204 , can extend at least partially through a bottom face of the strut  204 , can extend at least partially through a side face of the strut  204 , or can extend entirely across the strut  204 . The segment including the at least one flexure  216  can also include a clasp  210  to set the position, angle, location, or the like along the given axis (for example, the z-axis) of the segment including the securing block  100  and the at least one flexure  216  once the desired position, angle, location, or the like has been determined. The clasp  210  can include, but is not limited to, screws, dowels, rods, and rivets. The clasp  210  can also be used to adjust position, angle, location, or the like of the segment including the at least one flexure  216 . In one embodiment, the distal end  212  of the strut  204  can be adjusted (i.e., angle, position, location, or the like), such as by translating, flexing, bending, twisting, or moving, relative to, and/or independently of, the proximal end of the strut  204 . The distal end  212  can also include at least one securing block  100 . In one embodiment, the proximal end of the strut  204  can be adjusted (i.e., angle, position, location, or the like), such as by translating, flexing, bending, twisting, or moving, relative to, and/or independently of, the distal end  212  of the strut  204 . The proximal end can also include at least one securing block  100 . 
       FIGS. 3A-3B  show a secure bar  300 . The secure bar  300  is similar to the secure bar  200 , except that secure bar  300  includes a projection  306  at a distal end of a bracket  302 . The secure bar  300  includes the bracket  302  and a strut  304 . The strut  304  includes a proximal end including a first securing block  100  and a distal end  310  including a second securing block  100 . In one embodiment, the bracket  302  and the strut  304  are a single piece. In one embodiment, the bracket  302  and the strut  304  are separate pieces. When the bracket  302  and the strut  304  are separate pieces, the bracket  302  and the strut  304  can be attached, adjoined, or adhered to each other by screws, dowels, rods, at least one dovetail joint, at least one tongue-and-groove joint, an adhesive, an epoxy, at least one magnet, or by any other appropriate manner in which to attach, adjoin, or adhere two or more pieces. 
     In one embodiment, the bracket  302  can include at least one bearing  308  to permit rotation of the bracket  302  relative to a substrate (not shown) or at least one other component, including, without limitation, the strut  304  or a frame (not shown). 
     In one embodiment, the bracket  302  includes a projection  306  extending outwardly from the bracket  302 . In one embodiment, the bracket  302  includes two projections  306  extending outwardly, in the same direction, from the bracket  302 . 
     In one embodiment, the secure bar  300  can include at least one flexure  312 , such as one or more flexible materials, a cut-out (e.g., a track, a groove, a notch, or the like), a material less stiff than the other material composing the strut  304 , or the like. The at least one flexure  312  allows for movement of the securing block  100  via the proximal end or the distal end  310  of the secure bar  300  to move along a given axis (for example, the z-axis) relative to the other end of the secure bar  300 . The at least one flexure  312  can extend at least partially through a top face of the strut  304 , can extend at least partially through a bottom face of the strut  304 , can extend at least partially through a side face of the strut  304 , or can extend entirely across the strut  304 . The segment including the at least one flexure  312  can also include a clasp to set the position, angle, location, or the like along the given axis (for example, the z-axis) of the segment including the securing block  100  and the at least one flexure  312  once the desired position, angle, location, or the like has been determined. The clasp can include, but is not limited to, screws, dowels, rods, and rivets. The clasp can also be used to adjust position, angle, location, or the like of the segment including the at least one flexure  312 . In one embodiment, the distal end  310  of the strut  304  can be adjusted (i.e., angle, position, location, or the like), such as by translating, flexing, bending, twisting, or moving, relative to, and/or independently of, the proximal end of the strut  304 . The distal end  310  can also include at least one securing block  100 . In one embodiment, the proximal end of the strut  304  can be adjusted (i.e., angle, position, location, or the like), such as by translating, flexing, bending, twisting, or moving, relative to, and/or independently of, the distal end  310  of the strut  304 . The proximal end can also include at least one securing block  100 . 
     Holder 
       FIGS. 4A-4C  show a holder  400 . The holder  400  includes a frame  402  and the secure bar  200 . The frame  402  includes a first arm  404  and a second arm  406 . The secure bar  200  can move, rotate, or translate freely and independently of the frame  402 , such as having an open position (i.e., to permit the insertion of a substrate) and a closed position (i.e., to permit the secure holding of the substrate). The holder  400  includes a cavity  412  which is formed by the secure bar  200  and the frame  402 , and which is sized and shaped to fit and accept a substrate (not shown). The first and second arms  404 ,  406  are joined at proximal ends. In one embodiment, the first arm  404  is longer than the second arm  406 . In one embodiment, the second arm  406  is longer than the first arm  404 . In one embodiment, the first and second arms  404 ,  406  are equal in length. In one embodiment, the frame  402  is a single piece. In one embodiment, the frame  402  is at least two pieces, such that the first arm  404  is part of or fully forms a first piece and the second arm  406  is part of or fully forms a second piece. 
     It should be noted that the movement, rotation, or translation of the secure bar  200 ,  300  from the open position to closed position (and vice versa) causes the entirety of the secure bar  200 ,  300  to move, rotate, or translate. However, the movement, rotation, or translation of the secure bar  200 ,  300  is different than the movement or adjustment of the at least one securing block via the proximal or distal ends along a given axis. Therefore, adjustment of at least one securing block  100  along a given axis is such that at least one securing block  100  can be adjusted (i.e., angle, position, location, or the like) by translating, flexing, bending, twisting, or moving the segment of the secure bar  200 ,  300  or the frame  402 ,  502  on which the at least one securing block  100  is located relative to, and/or independently of, other segments of the secure bar  200 ,  300  or the frame  402 ,  502 . In other words, for example, the entirety of the secure bar  200 ,  300  can have a first motion for opening and closing, and then a segment of the secure bar  200 ,  300  can have a second motion for adjusting the at least one securing block  100  located on or at that segment. 
     The secure bar  200  is proximal to the distal end of the first arm  404 . In one embodiment, the first arm  404  is connected to the strut  204  of the secure bar  200 . In one embodiment, the first arm  404  is connected to the bracket  202  of the secure bar  200 . In one embodiment, the first arm  404  is connected to at least one bearing  208  of the secure bar  200 . 
     In one embodiment, an inner surface of the first arm  404  includes a platform  410  at least partially located between the proximal and distal ends. The platform  410  can support the substrate (not shown). 
     The second arm  406  includes at least two securing blocks  100 . In one embodiment, each of the securing blocks  100  of the second arm  406  are directly across the cavity  412  from the corresponding securing blocks  100  of the secure arm  200 . In one embodiment, at least one of the securing blocks  100  is not directly across the cavity  412  from the corresponding securing block  100  of the secure bar  200 . In one embodiment, the distal end of second arm  406  includes a pedestal  408  extending towards the secure bar  200 . The pedestal  200  can support the substrate (not shown). 
     The secure bar  200  has an open position and a closed position. In one embodiment, the secure bar  200  of the holder  400  is moved from the open position to the closed position, and vice versa, by a motor (not shown, see  FIGS. 6A-6D ). The motor (not shown) is adjoined to or connected to the at least one projection  206  and/or the flange  214 . In one embodiment, the motor (not shown) is adjoined to or connected to the at least one projection  206  and/or the flange  214  by a coupling (not shown). In one embodiment, the motor (not shown) is directly adjoined to or connected to the at least one projection  206  and/or the flange  214 . 
     In one embodiment, at least one securing block  100  is adjustable along a given axis (for example, the z-axis), while the other securing blocks  100  are stationary, excluding adjusting the height relative to the frame  402  or the secure bar  200 . In one embodiment, all of the securing blocks  100  are adjustable along a given axis (for example, the z-axis). 
     In one embodiment, at least one of the first or second arms  404 ,  406  of the frame  402  includes at least one flexure (not shown), such as one or more flexible materials, a cut-out (e.g., a track, a groove, a notch, or the like), a material less stiff than the other material composing the frame, or the like. For example, the second arm can include the at least one flexure (not shown) to allow for movement of the securing block  100  via the proximal end or the distal end of the second arm  406  (depending on the location of the at least one flexure within the second arm  406 , for example) to move along a given axis (for example, the z-axis) relative to the other end of the second arm  406 . The at least one flexure (not shown) can extend at least partially through a top face of the second arm  406 , can extend at least partially through a bottom face of the second arm  406 , can extend at least partially through a side face of the second arm  406 , or can extend entirely across the second arm  406 . The segment including the at least one flexure (not shown) can also include a clasp (not shown) to set the position, angle, location, or the like along the given axis (for example, the z-axis) of the segment including the securing block  100  and the at least one flexure (not shown) once the desired position, angle, location, or the like has been determined. The clasp (not shown) can include, but is not limited to, screws, dowels, rods, and rivets. The clasp (not shown) can also be used to adjust position, angle, location, or the like of the segment including the at least one flexure (not shown). In one embodiment, the distal end of the second arm  406  can be adjusted (i.e., angle, position, location, or the like), such as by translating, flexing, bending, twisting, or moving, relative to, and/or independently of, the proximal end of the second arm  406 . The distal end can also include at least one securing block  100 . In one embodiment, the proximal end of the second arm  406  can be adjusted (i.e., angle, position, location, or the like), such as by translating, flexing, bending, twisting, or moving, relative to, and/or independently of, the distal end of the second arm  406 . The proximal end can also include at least one securing block  100 . 
     In one embodiment, all of the securing blocks  100  have the same configuration and design. In one embodiment, at least one of the securing blocks  100  has a configuration and/or design different than the other securing blocks  100 . 
       FIGS. 5A-5C  show a holder  500 . The holder  500  is similar to the holder  400 , except that the holder  500  includes the secure bar  300 . The holder  500  includes a frame  502  and the secure bar  300 . The frame  502  includes a first arm  504  and a second arm  506 . The secure bar  300  can move, rotate, or translate freely and independently of the frame  502 . The holder  500  includes a cavity  514  which is formed by the secure bar  300  and the frame  502 , and which is sized and shaped to fit and accept a substrate (not shown). The first and second arms  504 ,  506  are joined at proximal ends. In one embodiment, the first arm  504  is longer than the second arm  506 . In one embodiment, the second arm  506  is longer than the first arm  504 . In one embodiment, the first and second arms  504 ,  506  are equal in length. In one embodiment, the frame  502  is a single piece. In one embodiment, the frame  502  is at least two pieces, such that the first arm  504  is part of or fully forms a first piece and the second arm  506  is part of or fully forms a second piece. 
     The secure bar  300  is proximal to the distal end of the first arm  504 . In one embodiment, the first arm  504  is connected to the strut  304  of the secure bar  300 . In one embodiment, the first arm  504  is connected to the bracket  302  of the secure bar  300 . In one embodiment, the first arm  504  is connected to at least one bearing  208  of the secure bar  300 . 
     In one embodiment, an inner surface of the first arm  504  includes a platform  510  at least partially located between the proximal and distal ends. The platform  510  can support the substrate (not shown). 
     The second arm  504  includes at least two securing blocks  100 . In one embodiment, each of the securing blocks  100  of the second arm  506  are directly across the cavity  514  from the corresponding securing blocks  100  of the secure bar  300 . In one embodiment, at least one of the securing blocks  100  is not directly across the cavity  514  from the corresponding securing block  100  of the secure bar  300 . In one embodiment, the distal end of second arm  506  includes a pedestal  508  extending towards the secure bar  300 . The pedestal  508  can support the substrate (not shown). 
     The secure bar  300  can also include a spring  512  to pre-load the secure bar  300  and/or at least one bearing  308  of the secure bar  300 . In one embodiment, the spring  512  is connected to the first arm  504 . In one embodiment, the spring  512  is connected to another element, such as a mount (not shown, see  FIGS. 7A-7B ). In one embodiment, the spring  512  is connected to the first arm  504  and the mount (not shown, see  FIGS. 7A-7B ). 
     In one embodiment, though the distal end  310  is adjustable, the other securing blocks  100  are stationary, excluding adjusting the height relative to the frame  502  or the secure bar  300 . 
     In one embodiment, at least one of the first or second arms  504 ,  506  of the frame  502  includes at least one flexure (not shown), such as one or more flexible materials, a cut-out (e.g., a track, a groove, a notch, or the like), a material less stiff than the other material composing the frame, or the like. For example, the second arm can include the at least one flexure (not shown) to allow for movement of the securing block  100  via the proximal end or the distal end of the second arm  506  (depending on the location of the at least one flexure within the second arm  506 , for example) to move along a given axis (for example, the z-axis) relative to the other end of the second arm  506 . The at least one flexure (not shown) can extend at least partially through a top face of the second arm  506 , can extend at least partially through a bottom face of the second arm  506 , can extend at least partially through a side face of the second arm  506 , or can extend entirely across the second arm  506 . The segment including the at least one flexure (not shown) can also include a clasp (not shown) to set the position, angle, location, or the like along the given axis (for example, the z-axis) of the segment including the securing block  100  and the at least one flexure (not shown) once the desired position, angle, location, or the like has been determined. The clasp (not shown) can include, but is not limited to, screws, dowels, rods, and rivets. The clasp (not shown) can also be used to adjust position, angle, location, or the like of the segment including the at least one flexure (not shown). In one embodiment, the distal end of the second arm  506  can be adjusted (i.e., angle, position, location, or the like), such as by translating, flexing, bending, twisting, or moving, relative to, and/or independently of, the proximal end of the second arm  506 . The distal end can also include at least one securing block  100 . In one embodiment, the proximal end of the second arm  506  can be adjusted (i.e., angle, position, location, or the like), such as by translating, flexing, bending, twisting, or moving, relative to, and/or independently of, the distal end of the second arm  506 . The proximal end can also include at least one securing block  100 . 
     In one embodiment, all of the securing blocks  100  have the same configuration and design. In one embodiment, at least one of the securing blocks  100  has a configuration and/or design different than the other securing blocks  100 . 
       FIGS. 6A-6D  show a holder  600 . In one embodiment, as shown in  FIGS. 6A-6D , the holder  600  includes two holders  400 ,  500 . In one embodiment, the holder  600  includes two holders, both of which are the holder  400 . In one embodiment, the holder  600  includes two holders, both of which are the holder  500 . 
     The holder  600  includes a motor  602  and a coupling  608 . Though the motor  602  and the coupling  608  are shown in  FIGS. 6A-6D  for the holder  600 , the motor  602  and the coupling  608  can be used in any embodiment of any holder discussed herein or any equivalent thereof. 
     In one embodiment, the holder  600  includes a slider  604  and a connector  606 . The slider  604  is adjoined or connected to the secure bar  200 . The connector  606  can be adjoined to a mount (not shown). A portion of the slider  604  can be fit within a track or groove of the connector  606 , thereby permitting movement of the slider  604  along a single or given plane, such that the secure bar  200  can translate between the open and closed positions via the motor  602  and the coupling  608 . Though the slider  604  and the connector  606  are shown in  FIGS. 6A-6D  for the holder  600 , the slider  604  and the connector  606  can be used in any embodiment of any holder discussed herein or any equivalent thereof. 
     In one embodiment, a leaf spring (not shown) can be used in place of both the slider  604  and the connector  606 . 
     In one embodiment, the holder  600  can be attached to a secondary device, such as a scanner or an imaging microscope or a fluorescence microscope, for imaging and/or processing by a mount  702  (i.e. a screw, a nail, a peg, a pin, a nut and bolt, a dowel, a staple, a rivet, or the like), by an adhesive, by welding, by clips, by detents, by tongue and groove joint, or the like. The holder  600  and/or the mount  702  can include at least one hole to accommodate an attachment mechanism for proper, secure attachment to the secondary device. Though the mount  702  is shown in  FIGS. 7A-7B  for the holder  600 , the mount  702  can be used in any embodiment of any holder discussed herein or any equivalent thereof. In one embodiment, the holder  600  can be attached to a secondary device for imaging and/or processing by the attachment mechanism (i.e. a screw, a nail, a peg, a pin, a nut and bolt, a dowel, a staple, a rivet, or the like), by an adhesive, by welding, by clips, by detents, by tongue and groove joint, or the like. The holder  600  can include at least one hole to accommodate the attachment mechanism for proper, secure attachment to the secondary device. Though the attachment mechanism is discussed for the holder  600 , the attachment mechanism can be used in any embodiment of any holder discussed herein or any equivalent thereof. 
     In one embodiment, the mount  702  can be adjusted, such as by the attachment mechanisms, to be planar (i.e., parallel to or coplanar with) with an imaging plane (for example, a x-y plane). 
     Method 
     For the sake of convenience, the methods are described with reference to a slide as an example substrate and with the holder  400 . But the methods described below are not intended to be so limited in their scope of application. The methods, in practice, may be used with any kind of substrate (for example, without limitation, a well plate) and with any embodiment of holder, secure bar, or securing block. 
     In one embodiment, a reference slide is inserted into the holder  400 . The reference slide can be placed onto the platform  410  and the pedestal  408  or can be placed in direct contact at least two of the securing blocks  100 . The secure bar  200  is in the open position. The secure bar  200  is moved into the closed position. The ramps  106  of the respective securing blocks  100  lift the reference slide up and the stoppers  104  of the respective securing blocks  100  set the maximum lift distance for the reference slide and constrain the reference slide. When the secure bar  200  is set in the closed position, the force exerted on the reference slide by the securing blocks  100  inhibits translational movement of the reference slide relative to the holder  400 . The securing block  100  at the distal end  212  of the secure bar  200  can be adjusted to account for any warpage or deformations in the reference slide, thereby making the reference slide planar or substantially planar with an imaging plane (for example, the x-y plane). Once the reference slide is secured, such that the planarity of the reference slide is confirmed or observed in any appropriate manner, the secure bar is moved into the open position to release the forces on the slide. The ramps of the respective securing blocks guide the slide down. The reference slide is then removed from the holder and an experimental slide (i.e., a slide include a sample to be imaged) is inserted to restart the process. 
     In one embodiment, the method discussed above excludes a reference slide and is done on a slide-by-slide basis (i.e., inserted, secure bar closed, securing block adjusted, etc.). In one embodiment, the method discussed above excludes a reference slide and is done for the first experimental slide with the adjustment to the securing block  100  being retained for all subsequent experimental slides. 
     When a feature or element is herein referred to as being “on” another feature or element, it can be directly on the other feature or element or intervening features and/or elements may also be present. In contrast, when a feature or element is referred to as being “directly on” another feature or element, there are no intervening features or elements present. It will also be understood that, when a feature or element is referred to as being “connected”, “attached” or “coupled” to another feature or element, it can be directly connected, attached or coupled to the other feature or element or intervening features or elements may be present. In contrast, when a feature or element is referred to as being “directly connected”, “directly attached” or “directly coupled” to another feature or element, there are no intervening features or elements present. Although described or shown with respect to one embodiment, the features and elements so described or shown can apply to other embodiments. It will also be appreciated by those of skill in the art that references to a structure or feature that is disposed “adjacent” another feature may have portions that overlap or underlie the adjacent feature. 
     Terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. For example, as used herein, the singular forms “a”, “an” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms “comprises” and/or “comprising,” when used in this specification, specify the presence of stated features, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, steps, operations, elements, components, and/or groups thereof. As used herein, the term “and/or” includes any and all combinations of one or more of the associated listed items and may be abbreviated as “/”. 
     Spatially relative terms, such as “under”, “below”, “lower”, “over”, “upper” and the like, may be used herein for ease of description to describe one element or feature&#39;s relationship to another element(s) or feature(s) as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is inverted, elements described as “under” or “beneath” other elements or features would then be oriented “over” the other elements or features. Thus, the exemplary term “under” can encompass both an orientation of over and under. The device may be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly. Similarly, the terms “upwardly”, “downwardly”, “vertical”, “horizontal” and the like are used herein for the purpose of explanation only unless specifically indicated otherwise. 
     Although the terms “first” and “second” may be used herein to describe various features/elements (including steps), these features/elements should not be limited by these terms, unless the context indicates otherwise. These terms may be used to distinguish one feature/element from another feature/element. Thus, a first feature/element discussed below could be termed a second feature/element, and similarly, a second feature/element discussed below could be termed a first feature/element without departing from the teachings of the present invention. Additionally, though “first” and “second” are used, the terms are not intended to limit various features/elements to only one or two. Rather, three (i.e., third), four (i.e., fourth), or more may be included or used where appropriate or desirous to do so. 
     Throughout this specification and the claims which follow, unless the context requires otherwise, the word “comprise”, and variations such as “comprises” and “comprising” means various components can be co-jointly employed in the methods and articles (e.g., compositions and apparatuses including device and methods). For example, the term “comprising” will be understood to imply the inclusion of any stated elements or steps but not the exclusion of any other elements or steps. 
     As used herein in the specification and claims, including as used in the examples and unless otherwise expressly specified, all numbers may be read as if prefaced by the word “about” or “approximately,” even if the term does not expressly appear. The phrase “about” or “approximately” may be used when describing magnitude and/or position to indicate that the value and/or position described is within a reasonable expected range of values and/or positions. For example, a numeric value may have a value that is +/−0.1% of the stated value (or range of values), +/−1% of the stated value (or range of values), +/−2% of the stated value (or range of values), +/−5% of the stated value (or range of values), +/−10% of the stated value (or range of values), etc. Any numerical values given herein should also be understood to include about or approximately that value, unless the context indicates otherwise. For example, if the value “10” is disclosed, then “about 10” is also disclosed. Any numerical range recited herein is intended to include all sub-ranges subsumed therein. It is also understood that when a value is disclosed that “less than or equal to” the value, “greater than or equal to the value” and possible ranges between values are also disclosed, as appropriately understood by the skilled artisan. For example, if the value “X” is disclosed the “less than or equal to X” as well as “greater than or equal to X” (e.g., where X is a numerical value) is also disclosed. It is also understood that the throughout the application, data is provided in a number of different formats, and that this data, represents endpoints and starting points, and ranges for any combination of the data points. For example, if a particular data point “10” and a particular data point “15” are disclosed, it is understood that greater than, greater than or equal to, less than, less than or equal to, and equal to 10 and 15 are considered disclosed as well as between 10 and 15. It is also understood that each unit between two particular units are also disclosed. For example, if 10 and 15 are disclosed, then 11, 12, 13, and 14 are also disclosed. 
     Although various illustrative embodiments are described above, any of a number of changes may be made to various embodiments without departing from the scope of the invention as described by the claims. For example, the order in which various described method steps are performed may often be changed in alternative embodiments, and in other alternative embodiments one or more method steps may be skipped altogether. Optional features of various device and system embodiments may be included in some embodiments and not in others. Therefore, the foregoing description is provided primarily for exemplary purposes and should not be interpreted to limit the scope of the invention as it is set forth in the claims. 
     The examples and illustrations included herein show, by way of illustration and not of limitation, specific embodiments in which the subject matter may be practiced. As mentioned, other embodiments may be utilized and derived there from, such that structural and logical substitutions and changes may be made without departing from the scope of this disclosure. Such embodiments of the inventive subject matter may be referred to herein individually or collectively by the term “invention” merely for convenience and without intending to voluntarily limit the scope of this application to any single invention or inventive concept, if more than one is, in fact, disclosed. Thus, although specific embodiments have been illustrated and described herein, any arrangement calculated to achieve the same purpose may be substituted for the specific embodiments shown. This disclosure is intended to cover any and all adaptations or variations of various embodiments. Combinations of the above embodiments, and other embodiments not specifically described herein, will be apparent to those of skill in the art upon reviewing the above description. 
     The foregoing description, for purposes of explanation, used specific nomenclature to provide a thorough understanding of the disclosure. However, it will be apparent to one skilled in the art that the specific details are not required in order to practice the systems and methods described herein. The foregoing descriptions of specific embodiments are presented by way of examples for purposes of illustration and description. They are not intended to be exhaustive of or to limit this disclosure to the precise forms described. Many modifications and variations are possible in view of the above teachings. The embodiments are shown and described in order to best explain the principles of this disclosure and practical applications, to thereby enable others skilled in the art to best utilize this disclosure and various embodiments with various modifications as are suited to the particular use contemplated. It is intended that the scope of this disclosure be defined by the following claims and their equivalents: