Patent Publication Number: US-2020289039-A1

Title: Fluid control devices and methods of using the same

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application claims priority to and the benefit of U.S. Provisional Patent Application Ser. No. 62/816,477 entitled, “Fluid Control Devices and Methods of Using the Same,” filed Mar. 11, 2019, the disclosure of which is incorporated herein by reference in its entirety. 
    
    
     BACKGROUND 
     Embodiments described herein relate generally to the procurement of bodily fluid samples, and more particularly to fluid diversion, sequestration, and/or isolation devices and methods for procuring bodily fluid samples with reduced contaminants such as dermally residing microbes and/or other contaminants exterior to the bodily fluid source. 
     Health care practitioners routinely perform various types of microbial as well as other broad diagnostic tests on patients using parenterally obtained bodily fluids. As advanced diagnostic technologies evolve and improve, the speed, accuracy (both sensitivity and specificity), and value of information that can be provided to clinicians continues to improve. Maintaining the integrity of the bodily fluid sample during and/or after collection ensures that analytical diagnostic results are representative of the in vivo conditions of a patient. Examples of diagnostic technologies that are reliant on high quality, non-contaminated, and/or unadulterated bodily fluid samples include but are not limited to microbial detection, molecular diagnostics, genetic sequencing (e.g., deoxyribonucleic acid (DNA), ribonucleic acid (RNA), next-generation sequencing (NGS), etc.), biomarker identification, and the like. 
     Inaccurate results from such testing, can result from the presence of biological matter—including cells external to the intended sample source and/or other external contaminants—that inadvertently are included in the bodily fluid sample being analyzed. In short, when the purity of the bodily fluid sample is compromised during the specimen procurement process, resultant analytical test results may be inaccurate, distorted, adulterated, falsely positive, falsely negative, and/or otherwise not representative of the actual condition of the patient. In turn, these results can lead to faulty, inaccurate, confused, unsure, low confidence, and/or otherwise undesired clinical decision-making. 
     In some instances, devices and/or systems can be used to reduce the likelihood of contamination, adulteration, and/or the like of bodily fluid samples for testing. For example, some known devices can be configured to collect, divert, separate, and/or sequester (e.g., isolate) an initial volume of bodily fluid that may be more likely to contain contaminants such as dermally residing microbes or the like. Some such devices, however, can be cumbersome, non-intuitive, perceived as difficult to use, inappropriate or unusable for a target patient population, etc. In addition, some such devices can require training, user observation, intervention by more than one user, and/or can otherwise present challenges that can lead to limited efficacy. In some instances, these and/or other challenges can complicate the collection of consistently high quality samples that are non-contaminated, sterile, unadulterated, etc., which in turn, can influence the validity of test result outcomes. 
     Some known devices and/or systems may be configured to limit an amount of user intervention by passively diverting an initial volume of bodily fluid, however, some such devices and/or systems may fail to adequately divert, sequester, and/or isolate a clinically desired and/or efficacious initial volume of bodily fluid (e.g., a pre-sample volume). Moreover, in some instances, the operation of some known devices and/or systems is dependent on a positive pressure applied or supplied by a bodily fluid source (e.g., a patient&#39;s blood pressure). In some such instances, however, the positive pressure may be insufficient to result in desirable flow dynamics and/or flow rates that make the use of such devices practical in various clinical settings such as, for example, emergency rooms and other intensive settings. 
     As such, a need exists for fluid control and/or diversion devices and methods for procuring bodily fluid samples with reduced contaminants such as dermally residing microbes and/or other contaminants exterior to the bodily fluid source that result in consistent bodily fluid collection (e.g., from a general patient population and/or a challenging patient population). In addition, a need exists for devices and methods that include, for example, bodily fluid collection with the assistance of various sources of external energy and/or negative pressure. 
     SUMMARY 
     Devices and methods for procuring bodily fluid samples with reduced contaminants such as dermally residing microbes and/or other contaminants exterior to the bodily fluid source are described herein. In some embodiments an apparatus for procuring bodily fluid samples with reduced contamination includes a housing, an actuator, and a flow controller. The housing forms at least a portion of a sequestration chamber, and has an inlet configured to be fluidically coupled to a bodily fluid source, and an outlet configured to be fluidically coupled to a fluid collection device. The fluid collection device exerts a suction force in at least a portion of the housing when fluidically coupled to the outlet. The actuator is coupled to the housing and has a first configuration in which the inlet is in fluid communication with the sequestration chamber, and a second configuration in which the inlet is in fluid communication with the outlet and is fluidically isolated from the sequestration chamber. The flow controller is disposed in the housing and defines a portion of the sequestration chamber. The flow controller can assume a first state in which the portion of the sequestration chamber has a first volume, and a second state in which the portion of the sequestration chamber has a second volume greater than the first volume. When the actuator is in the first configuration, the flow controller is configured to transition from the first state to the second state in response to the suction force to draw an initial volume of bodily fluid into the portion of the sequestration chamber. The actuator is configured to be transitioned to the second configuration after the initial volume of bodily fluid is drawn into the sequestration chamber to (1) sequester the sequestration chamber from the inlet, and (2) allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a schematic illustration of a fluid control device according to an embodiment. 
         FIGS. 2 and 3  are a front perspective view and a rear perspective view, respectively, of a fluid control device according to an embodiment. 
         FIGS. 4 and 5  are a side view and a top view, respectively, of the fluid control device of  FIG. 2 . 
         FIG. 6  is an exploded perspective view of the fluid control device of  FIG. 2 . 
         FIGS. 7 and 8  are each a cross-sectional view of the fluid control device of  FIG. 2  taken along the line  7 - 7  in  FIG. 4  and the line  8 - 8  in  FIG. 5 , respectively, shown in a first state. 
         FIGS. 9 and 10  are each a cross-sectional view of the fluid control device of  FIG. 2  taken along the line  7 - 7  in  FIG. 4  and the line  8 - 8  in  FIG. 5 , respectively, shown in a second state. 
         FIG. 11  is a partial cross-sectional view of the fluid control device of  FIG. 2  taken along the line  8 - 8  in  FIG. 5 , shown in the second state. 
         FIGS. 12 and 13  are a front perspective view and a rear perspective view, respectively, of a fluid control device according to an embodiment. 
         FIGS. 14 and 15  are a side view and a top view, respectively, of the fluid control device of  FIG. 12 . 
         FIG. 16  is an exploded perspective view of the fluid control device of  FIG. 12 . 
         FIGS. 17 and 18  are each a cross-sectional view of the fluid control device of  FIG. 12  taken along the line  17 - 17  in  FIG. 14  and the line  18 - 18  in  FIG. 15 , shown in a first state. 
         FIGS. 19 and 20  are each a cross-sectional view of the fluid control device of  FIG. 12  taken along the line  17 - 17  in  FIG. 14  and the line  18 - 18  in  FIG. 15 , shown in a second state. 
         FIG. 21  is a partial cross-sectional view of the fluid control device of  FIG. 12  taken along the line  18 - 18  in  FIG. 15 , shown in the second state. 
         FIGS. 22 and 23  are a front perspective view and a rear perspective view, respectively, of a fluid control device according to an embodiment. 
         FIGS. 24 and 25  are each a cross-sectional view of the fluid control device of  FIG. 22  taken along the line  24 - 24  and shown in a first state. 
         FIGS. 26 and 27  are each a cross-sectional view of the fluid control device of  FIG. 22  taken along the line  24 - 24  and shown in a second state. 
         FIG. 28  is a flowchart illustrating a method of using a fluid control device according to an embodiment. 
     
    
    
     DETAILED DESCRIPTION 
     Devices and methods for collecting, diverting, sequestering, isolating, etc. an initial volume of bodily fluid to reduce contamination in subsequently procured bodily fluid samples are described herein. Any of the fluid control devices described herein can be configured to receive, procure, and/or transfer a flow, bolus, volume, etc., of bodily fluid. A first reservoir, channel, flow path, or portion of the device can receive an initial amount of the bodily fluid flow, which then can be substantially or fully sequestered therein (e.g., contained or retained, circumvented, isolated, segregated, vapor-locked, separated, and/or the like). In some instances, contaminants such as dermally residing microbes or the like can be included and/or entrained in the initial amount of the bodily fluid and likewise are sequestered in or by the first reservoir or first portion of the device. Once the initial amount is sequestered, any subsequent amount of the bodily fluid flow can be diverted, channeled, directed, and/or otherwise allowed to flow to or through a second portion of the device, and/or any additional flow path(s). Based at least in part on the initial amount being sequestered, the subsequent amount(s) of bodily fluid can be substantially free from contaminants that may otherwise produce inaccurate, distorted, adulterated, and/or false results in some diagnostics and/or testing. In some instances, the initial amount of bodily fluid also can be used, for example, in other testing such as those less affected by the presence of contaminants, can be discarded as a waste volume, can be infused back into the patient, and/or can be used for any other suitable clinical application. 
     In some embodiments, a feature of the fluid control devices and/or methods described herein is the use of an external negative pressure source (e.g., provided by a fluid collection device or any other suitable means) that can (1) overcome physical patient challenges which can limit and/or prevent a sufficient pressure differential to fully engage the sequestration chamber and/or to transition fluid flow to the fluid collection device (e.g., a differential in blood pressure to ambient air pressure); (2) result in proper filling of the sequestration chamber with a clinically validated and/or desirable volume of bodily fluid; (3) result in efficient, timely, and/or user-accepted consistency with the bodily fluid collection process; and/or (4) provide a means of transitioning fluid flow (e.g., automatically or by manipulation to move any number of physical components of the system or by changing, switching, engaging, and/or otherwise providing desired fluid flow dynamics) to enable sequestration and/or isolation of the initial amount (e.g., a pre-sample) and collection of a subsequent sample. 
     In some embodiments, for example, an apparatus for procuring bodily fluid samples with reduced contamination includes a housing, an actuator, and a flow controller. The housing forms at least a portion of a sequestration chamber, and has an inlet configured to be fluidically coupled to a bodily fluid source, and an outlet configured to be fluidically coupled to a fluid collection device. The fluid collection device exerts a suction force in at least a portion of the housing when fluidically coupled to the outlet. The actuator is coupled to the housing and has a first configuration in which the inlet is in fluid communication with the sequestration chamber, and a second configuration in which the inlet is in fluid communication with the outlet and is fluidically isolated from the sequestration chamber. The flow controller is disposed in the housing and defines a portion of the sequestration chamber. The flow controller has a first state in which the portion of the sequestration chamber has a first volume, and a second state in which the portion of the sequestration chamber has a second volume greater than the first volume. When the actuator is in the first configuration, the flow controller transitions from the first state to the second state in response to the suction force to draw an initial volume of bodily fluid into the portion of the sequestration chamber. The actuator is configured to be transitioned to the second configuration after the initial volume of bodily fluid is drawn into the sequestration chamber to (1) sequester the sequestration chamber from the inlet, and (2) allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force. 
     In some embodiments, an apparatus for procuring bodily fluid samples with reduced contamination includes a housing, an actuator, and a flow controller. The housing forms at least a portion of a sequestration chamber, and has an inlet configured to be fluidically coupled to a bodily fluid source, and an outlet configured to be fluidically coupled to a fluid collection device. The fluid collection device exerts a suction force in at least a portion of the housing when fluidically coupled to the outlet. The actuator is coupled to the housing and has a first configuration in which the inlet is in fluid communication with the sequestration chamber, and a second configuration in which the inlet is in fluid communication with the outlet and is fluidically isolated from the sequestration chamber. The flow controller is disposed in the housing and defines a portion of the sequestration chamber. The flow controller has a first a first state in which a first side of the flow controller is in contact with at least a portion of a first surface of the sequestration chamber, and a second state in which a second side of the flow controller is in contact with at least a portion of a second surface of the sequestration chamber, opposite the first surface. The flow controller transitions from the first state to the second state when the actuator is in the first configuration, as a result of the suction force being exerted on the second side of the flow controller to draw an initial volume of bodily fluid into a portion of the sequestration chamber defined between the first surface and the first side of the flow controller. The actuator is configured to be transitioned to the second configuration after the initial volume of bodily fluid is drawn into the sequestration chamber to (1) sequester the sequestration chamber from the inlet, and (2) allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force. 
     In some embodiments, a fluid control device can include a housing, a flow controller, and an actuator. The housing has an inlet and an outlet, and forms a sequestration chamber. The inlet is configured to be placed in fluid communication with a bodily fluid source. The outlet is configured to be placed in fluid communication with a fluid collection device configured to exert a suction force within at least a portion of the housing. The actuator is coupled to the housing and is configured to establish fluid communication between the inlet and the sequestration chamber when in a first state and to establish fluid communication between the inlet and the outlet when placed in a second state. The flow controller is disposed in the sequestration chamber and is configured to transition from a first state to a second state in response to the suction force when the actuator is in its first state to allow an initial volume of bodily fluid to flow into a portion of the sequestration chamber. The portion of the sequestration chamber has a first volume when the flow controller is in the first state and a second volume greater than the first volume when the flow controller is in the second state. The actuator is configured to be transitioned to its second state after the initial volume of bodily fluid is received in the portion of the sequestration chamber to (1) sequester the sequestration chamber, and (2) allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force. 
     In some embodiments, a method for procuring bodily fluid samples with reduced contamination using a fluid control device having a housing, an actuator, and a flow controller includes establishing fluid communication between a bodily fluid source and an inlet of the housing. A fluid collection device is coupled to an outlet of the housing and exerts a suction force within at least a portion of the housing when coupled to the outlet. The flow controller is transitioned from a first state to a second state in response to the suction force, increasing a volume of a sequestration chamber collectively defined by the flow controller and a portion of the housing. In response to the increase in volume, a first portion of the sequestration chamber receives a volume of air contained in a flow path defined between the bodily fluid source and the sequestration chamber, and a second portion of the sequestration chamber receives an initial volume of bodily fluid. The actuator is transitioned from a first configuration to a second configuration after receiving the initial volume of bodily fluid in the second portion of the sequestration chamber to (1) sequester the sequestration chamber and (2) allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force. 
     In some embodiments, a method for procuring a bodily fluid sample with reduced contamination using a fluid control device having a housing, a flow controller, and an actuator can include, for example, establishing fluid communication between a bodily fluid source and an inlet of the housing. A fluid collection device is fluidically coupled to an outlet of the housing. The flow controller is transitioned from a first state to a second state in response to a suction force exerted by the fluid collection device to increase a volume of a first portion of the sequestration chamber and a second portion of the sequestration chamber. The first portion of the sequestration chamber receives a volume of air contained in a flow path defined between the bodily fluid source and the sequestration chamber in response to the increase in the volume of the first portion of the sequestration chamber the second portion of the sequestration chamber. The second portion of the sequestration chamber receives an initial volume of bodily fluid in response to the increase in the volume of the first portion of sequestration chamber and the second portion of the sequestration chamber. After receiving the initial volume of bodily fluid in the second portion of the sequestration chamber, the actuator is transitioned from a first state to a second state to (1) sequester the sequestration chamber and (2) allow a subsequent volume of bodily fluid (e.g., the bodily fluid sample) to flow from the inlet to the outlet in response to the suction force. 
     Any of the embodiments and/or methods described herein can be used in the procurement of clean or substantially unadulterated bodily fluid samples such as, for example, blood samples. In some instances, bodily fluid samples (e.g., blood samples) can be tested for the presence of one or more potentially undesirable microbes, such as bacteria (e.g., Gram-Positive bacteria and/or Gram-Negative bacteria), fungi, yeast (e.g.,  Candida ), and/or the like. Various technologies can be employed to assist in the detection of the presence of microbes as well as other types of biological matter, specific types of cells, biomarkers, proteins, antigens, enzymes, blood components, and/or the like during diagnostic testing. Examples include but are not limited to molecular polymerase chain reaction (PCR), magnetic resonance and other magnetic analytical platforms, automated microscopy, spatial clone isolation, flow cytometry, whole blood (“culture free”) specimen analysis (e.g., NGS) and associated technologies, morphokinetic cellular analysis, and/or other common or evolving and advanced technologies to characterize patient specimens and/or to detect, identify, type, categorize, and/or characterize specific organisms, antibiotic susceptibilities, and/or the like. 
     For example, in some instances, microbial testing can include incubating patient samples in one or more vessels that may contain culture media (e.g., a nutrient rich and/or environmentally controlled medium to promote growth, and/or other suitable medium(s)), common additives, and/or other types of solutions conducive to microbial growth. Any microbes and/or organisms present in the patient sample flourish and/or grow over time in the culture medium (e.g., a variable amount of time from less than an hour to more than several days—which can be longer or shorter depending on the diagnostic technology employed). The presence of the microbes and/or organisms can be detected (e.g., by observing carbon dioxide levels and/or other detection methods) using automated, continuous monitoring, and/or other methods specific to the analytical platform or technology used for detection, identification, and/or the like. The presence of microbes and/or organisms in the culture medium suggests the presence of the same microbes and/or organisms in the patient sample, which in turn, suggests the presence of the same microbes and/or organisms in the bodily fluid of the patient from whom the sample was obtained. In other instances, a bodily fluid sample may be analyzed directly (i.e., not incubated) for the presence of microbes and/or organisms. When the presence of microbes is identified in the sample used for testing, the patient may be diagnosed and prescribed one or more antibiotics or other treatments specifically designed to treat or otherwise remove the undesired microbes and/or organisms from the patient. 
     Patient samples, however, can become contaminated during procurement and/or otherwise can be susceptible to false results. For example, microbes from a bodily surface (e.g., dermally residing microbes) that are dislodged during the specimen procurement process (e.g., either directly or indirectly via tissue fragments, hair follicles, sweat glands, and other skin adnexal structures) can be subsequently transferred to a culture medium, test vial, or other suitable specimen collection or transfer vessel with the patient sample and/or otherwise included in the specimen that is to be analyzed. Another possible source of contamination is from the person drawing the patient sample. For example, equipment, supplies, and/or devices used during a patient sample procurement process often include multiple fluidic interfaces (e.g., patient to needle, needle to transfer adapter, transfer adapter to sample vessel, catheter hub to syringe, syringe to transfer adapter, needle/tubing to sample vessels, and/or any other fluidic interface or any combination(s) thereof), each of which can introduce points of potential contamination. In some instances, such contaminants may thrive in a culture medium and/or may be otherwise identified, thereby increasing a risk or likelihood of a false positive microbial test result, which may inaccurately reflect the presence or lack of such microbes within the patient (i.e., in vivo). 
     Such inaccurate results because of contamination and/or adulteration are a concern when attempting to diagnose or treat a wide range of suspected illnesses, diseases, infections, patient conditions, and/or other maladies. For example, false results from microbial tests may lead to a patient being unnecessarily subjected to one or more anti-microbial therapies, and/or may lead to misdiagnosis and/or delayed treatment of a patient illness, any of which may cause serious side effects or consequences for the patient including, for example, death. As such, false results can produce an unnecessary burden and expense on the health care system due to extended length of patient stay and/or other complications associated with erroneous treatments. The use of diagnostic imaging equipment to arrive at these false results is also a concern from both a cost perspective and a patient safety perspective as unnecessary exposure to concentrated radiation associated with a variety of imaging procedures (e.g., CT scans) has many known adverse effects on long-term patient health. 
     As used in this specification and/or any claims included herein, the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, the term “a member” is intended to mean a single member or a combination of members, “a material” is intended to mean one or more materials, and/or the like. 
     As used herein, “bodily fluid” can include any fluid obtained directly or indirectly from a body of a patient. For example, “bodily fluid” includes, but is not limited to, blood, cerebrospinal fluid, urine, bile, lymph, saliva, synovial fluid, serous fluid, pleural fluid, amniotic fluid, mucus, sputum, vitreous, air, and/or the like, or any combination thereof. 
     As used herein, the words “proximal” and “distal” refer to the direction closer to and away from, respectively, a user who would place a device into contact with a patient. Thus, for example, the end of a device first touching the body of a patient would be a distal end of the device, while the opposite end of the device (e.g., the end of the device being manipulated by the user) would be a proximal end of the device. 
     As used herein, the terms “about,” “approximately,” and/or “substantially” when used in connection with stated value(s) and/or geometric structure(s) or relationship(s) is intended to convey that the value or characteristic so defined is nominally the value stated or characteristic described. In some instances, the terms “about,” “approximately,” and/or “substantially” can generally mean and/or can generally contemplate a value or characteristic stated within a desirable tolerance (e.g., plus or minus 10% of the value or characteristic stated). For example, a value of about 0.01 can include 0.009 and 0.011, a value of about 0.5 can include 0.45 and 0.55, a value of about 10 can include 9 to 11, and a value of about 1000 can include 900 to 1100. Similarly, a first surface may be described as being substantially parallel to a second surface when the surfaces are nominally parallel. While a value, structure, and/or relationship stated may be desirable, it should be understood that some variance may occur as a result of, for example, manufacturing tolerances or other practical considerations (such as, for example, the pressure or force applied through a portion of a device, conduit, lumen, etc.). Accordingly, the terms “about,” “approximately,” and/or “substantially” can be used herein to account for such tolerances and/or considerations. 
     As used herein, the terms “pre-sample,” “first,” and/or “initial,” can be used interchangeably to describe an amount, portion, or volume of bodily fluid that is collected and/or sequestered prior to procuring a “sample” volume. A “pre-sample,” “first,” and/or “initial” volume can be a predetermined, defined, desired, and/or given amount of bodily fluid. For example, a predetermined and/or desired pre-sample volume of bodily fluid can be a drop of bodily fluid, a few drops of bodily fluid, a volume of about 0.1 milliliter (mL), about 0.2 mL, about 0.3 mL, about 0.4 mL, about 0.5 mL, about 1.0 mL, about 2.0 mL, about 3.0 mL, about 4.0 mL, about 5.0 mL, about 10.0 mL, about 20.0 mL, about 50.0 mL, and/or any volume or fraction of a volume therebetween. In other embodiments, a pre-sample volume can be greater than 50 mL or less than 0.1 mL. In some specific embodiments, a predetermined and/or desired pre-sample volume can be between about 0.1 mL and about 5.0 mL. In other embodiments, a pre-sample volume can be, for example, a combined volume of any number of lumen (e.g., lumen that form at least a portion of a flow path from the bodily fluid source to an initial collection chamber, portion, reservoir, etc.). 
     As used herein, the terms “sample,” “second,” and/or “subsequent” can be used interchangeably to describe an amount, portion, or volume of bodily fluid that is used, for example, in one or more sample or diagnostic tests. A “sample” volume can be either a random volume or a predetermined or desired volume of bodily fluid collected after collecting, sequestering, and/or isolating a pre-sample volume of bodily fluid. In some embodiments, a desired sample volume of bodily fluid can be about 10 mL to about 60 mL. In other embodiments, a desired sample volume of bodily fluid can be less than 10 mL or greater than 60 mL. In some embodiments, for example, a sample volume can be at least partially based on one or more tests, assays, analyses, and/or processes to be performed on the sample volume. 
     The embodiments described herein can be configured to transfer bodily fluid substantially free of contaminants to one or more fluid collection device(s). In some embodiments, a fluid collection device can include, but is not limited to, any suitable vessel, container, reservoir, bottle, adapter, dish, vial, syringe, device, diagnostic and/or testing machine, and/or the like. In some embodiments, a fluid collection device can be substantially similar to or the same as known sample containers such as, for example, a Vacutainer® (manufactured by Becton Dickinson and Company (BD)), a BacT/ALERT® SN or BacT/ALERT® FA (manufactured by Biomerieux, Inc.), and/or any suitable reservoir, vial, microvial, microliter vial, nanoliter vial, container, microcontainer, nanocontainer, and/or the like. In some embodiments, a fluid collection device can be substantially similar to or the same as any of the sample reservoirs described in U.S. Pat. No. 8,197,420 entitled, “Systems and Methods for Parenterally Procuring Bodily-Fluid Samples with Reduced Contamination,” filed Dec. 13, 2007 (“the 420 Patent”), the disclosure of which is incorporated herein by reference in its entirety. 
     In some embodiments, a fluid collection device can be devoid of contents prior to receiving a sample volume of bodily fluid. For example, in some embodiments, a fluid collection device or reservoir can define and/or can be configured to define or produce a vacuum or suction such as, for example, a vacuum-based collection tube (e.g., a Vacutainer®), a syringe, and/or the like. In other embodiments, a fluid collection device can include any suitable additives, culture media, substances, enzymes, oils, fluids, and/or the like. For example, a fluid collection device can be a sample or culture bottle including, for example, an aerobic or anaerobic culture medium. The sample or culture bottle can be configured to receive a bodily fluid sample, which can then be tested (e.g., after incubation via in vitro diagnostic (IVD) tests, and/or any other suitable test) for the presence of, for example, Gram-Positive bacteria, Gram-Negative bacteria, yeast, fungi, and/or any other organism. In some instances, if such a test of the culture medium yields a positive result, the culture medium can be subsequently tested using a PCR-based system to identify a specific organism. In some embodiments, a sample reservoir can include, for example, any suitable additive or the like in addition to or instead of a culture medium. Such additives can include, for example, heparin, citrate, ethylenediaminetetraacetic acid (EDTA), oxalate, sodium polyanethol sulfonate (SPS), and/or the like. In some embodiments, a fluid collection device can include any suitable additive or culture media and can be evacuated and/or otherwise devoid of air. 
     While the term “culture medium” can be used to describe a substance configured to react with organisms in a bodily fluid (e.g., microorganisms such as bacteria) and the term “additive” can be used to describe a substance configured to react with portions of the bodily fluid (e.g., constituent cells of blood, serum, synovial fluid, etc.), it should be understood that a sample reservoir can include any suitable substance, liquid, solid, powder, lyophilized compound, gas, etc. Moreover, when referring to an “additive” within a sample reservoir, it should be understood that the additive could be a culture medium, such as an aerobic culture medium and/or an anaerobic culture medium contained in a culture bottle, an additive and/or any other suitable substance or combination of substances contained in a culture bottle and/or any other suitable reservoir such as those described above. That is to say, the embodiments described herein can be used with any suitable fluid reservoir or the like containing any suitable substance or combination of substances. 
     The embodiments described herein and/or portions thereof can be formed or constructed of one or more biocompatible materials. In some embodiments, the biocompatible materials can be selected based on one or more properties of the constituent material such as, for example, stiffness, toughness, durometer, bioreactivity, etc. Examples of suitable biocompatible materials include metals, glasses, ceramics, or polymers. Examples of suitable metals include pharmaceutical grade stainless steel, gold, titanium, nickel, iron, platinum, tin, chromium, copper, and/or alloys thereof. A polymer material may be biodegradable or non-biodegradable. Examples of suitable biodegradable polymers include polylactides, polyglycolides, polylactide-co-glycolides (PLGA), polyanhydrides, polyorthoesters, polyetheresters, polycaprolactones, polyesteramides, poly(butyric acid), poly(valeric acid), polyurethanes, and/or blends and copolymers thereof. Examples of non-biodegradable polymers include nylons, polyesters, polycarbonates, polyacrylates, polysiloxanes (silicones), polymers of ethylene-vinyl acetates and other acyl substituted cellulose acetates, non-degradable polyurethanes, polystyrenes, polyvinyl chloride, polyvinyl fluoride, poly(vinyl imidazole), chlorosulphonate polyolefins, polyethylene oxide, and/or blends and copolymers thereof. 
     The embodiments described herein and/or portions thereof can include components formed of one or more parts, features, structures, etc. When referring to such components it should be understood that the components can be formed by a singular part having any number of sections, regions, portions, and/or characteristics, or can be formed by multiple parts or features. For example, when referring to a structure such as a wall or chamber, the structure can be considered as a single structure with multiple portions, or as multiple, distinct substructures or the like coupled to form the structure. Thus, a monolithically constructed structure can include, for example, a set of substructures. Such a set of substructures may include multiple portions that are either continuous or discontinuous from each other. A set of substructures can also be fabricated from multiple items or components that are produced separately and are later joined together (e.g., via a weld, an adhesive, or any suitable method). 
     While some of the embodiments are described herein as being used for procuring bodily fluid for one or more culture sample testing, it should be understood that the embodiments are not limited to such a use. Any of the embodiments and/or methods described herein can be used to transfer a flow of bodily fluid to any suitable device that is placed in fluid communication therewith. Thus, while specific examples are described herein, the devices, methods, and/or concepts are not intended to be limited to such specific examples. 
     Referring now to the drawings,  FIG. 1  is a schematic illustration of a fluid control device  100  according to an embodiment. Generally, the fluid control device  100  (also referred to herein as “control device” or “device”) is configured to withdraw bodily fluid from a patient. A first portion or amount (e.g., an initial amount) of the withdrawn bodily fluid is sequestered from a second portion or amount (e.g., a subsequent amount) of the withdrawn bodily fluid. In some instances, contaminants or the like can be sequestered within the first portion or amount, leaving the second portion or amount substantially free of contaminants. The second portion or amount of bodily fluid can then be used as a biological sample in one or more tests (e.g., a blood culture test or the like), as described in more detail herein. The first portion or amount of bodily fluid can be discarded as waste, reinfused into the patient, or used in any suitable test that is less likely to produce false, inaccurate, distorted, inconsistent, and unreliable results as a result of potential contaminants contained therein. 
     The control device  100  includes a housing  110 , a flow controller  140 , and an actuator  150 . The housing  110  of the device  100  can be any suitable shape, size, and/or configuration. For example, in some embodiments, the housing  110  can have a size that is at least partially based on an initial amount or volume of bodily fluid configured to be transferred into and/or sequestered within a portion of the housing  110 . In some embodiments, the housing  110  can have a size and/or shape configured to increase the ergonomics and/or ease of use associated with the device  100 . Moreover, in some embodiments, one or more portions of the housing  110  can be formed of a relatively transparent material configured to allow a user to visually inspect and/or verify a flow of bodily fluid through at least a portion of the housing  110 . 
     The housing  110  has and/or forms an inlet  113 , an outlet  114 , and a sequestration chamber  130 . The inlet  113  is configured to fluidically couple to a lumen-containing device, which in turn, can place the housing  110  in fluid communication with a bodily fluid source. For example, the housing  110  can be coupled to and/or can include a lumen-containing device that is in fluid communication with the inlet  113  and that is configured to be percutaneously disposed in a patient (e.g., a butterfly needle, intravenous (IV) catheter, peripherally inserted central catheter (PICC), intermediary lumen-containing device, and/or the like). Thus, bodily fluid can be transferred from the patient and/or other bodily fluid source to the housing  110  via the inlet  113 , as described in further detail herein. The outlet  114  can be placed in fluid communication with a fluid collection device  180  (e.g., a fluid or sample reservoir, syringe, evacuated container, culture bottle, etc.). As described in further detail herein, the control device  100  can be used and/or manipulated to selectively transfer a volume of bodily fluid from a bodily fluid source, through the inlet  113 , the housing  110 , and the outlet  114  to the fluid collection device  180 . 
     The housing  110  can define at least a portion of any number of fluid flow paths. For example, as shown in  FIG. 1 , the housing  110  defines one or more fluid flow paths  115  between the inlet  113  and the sequestration chamber  130  and/or one or more fluid flow paths  116  between the inlet  113  and the outlet  114 . As described in further detail herein, the control device  100  and/or the housing  110  can be configured to transition between any number of states, operating modes, and/or configurations to selectively control bodily fluid flow through at least one of the fluid flow paths  115  and/or  116 . Moreover, the control device  100  and/or the housing  110  can be configured to transition automatically (e.g., based on pressure differential, time, electronically, saturation of a membrane, an absorbent and/or barrier material, etc.) or via intervention (e.g., user intervention, mechanical intervention, or the like). 
     The sequestration chamber  130  is at least temporarily placed in fluid communication with the inlet  113  via the fluid flow path(s)  115 . As described in further detail herein, the sequestration chamber  130  is configured to (1) receive a flow and/or volume of bodily fluid from the inlet  113  and (2) sequester (e.g., separate, segregate, contain, retain, isolate, etc.) the flow and/or volume of bodily fluid therein. The sequestration chamber  130  can have any suitable arrangement such as, for example, those described herein with respect to specific embodiments. It should be understood, however, that the control device  100  and/or the housing  110  can have a sequestration chamber  130  arranged in any suitable manner and therefore, the sequestration chamber  130  is not intended to be limited to those shown and described herein. For example, in some embodiments, the sequestration chamber  130  can be at least partially formed by the housing  110 . In other embodiments, the sequestration chamber  130  can be a reservoir placed and/or disposed within a portion of the housing  110 . In other embodiments, the sequestration chamber  130  can be formed and/or defined by a portion of the fluid flow path  115 . That is to say, the housing  110  can define one or more lumens and/or can include one or more lumen defining device(s) configured to receive an initial flow or volume of bodily fluid from the inlet  113 , thereby forming and/or functioning as the sequestration chamber  130 . 
     The sequestration chamber  130  can have any suitable volume and/or fluid capacity. For example, in some embodiments, the sequestration chamber  130  can have a volume and/or fluid capacity between about 0.1 mL and about 5.0 mL. In some embodiments, the sequestration chamber  130  can have a volume measured in terms of an amount of bodily fluid (e.g., the initial or first amount of bodily fluid) configured to be transferred in the sequestration chamber  130 . For example, in some embodiments, the sequestration chamber  130  can have a volume sufficient to receive an initial volume of bodily fluid as small as a microliter or less of bodily fluid (e.g., a volume as small as 20 drops of bodily fluid, 10 drops of bodily fluid, 5 drops of bodily fluid, a single drop of bodily fluid, or any suitable volume therebetween). In other embodiments, the sequestration chamber  130  can have a volume sufficient to receive an initial volume of bodily fluid up to, for example, about 5.0 mL, 10.0 mL, 15.0 mL, 10.0 mL, 30.0 mL, 40.0 mL, 50.0 mL, or more. In some embodiments, the sequestration chamber  130  can have a volume that is equal to at least some of the volumes of one or more lumen(s) placing the sequestration chamber  130  in fluid communication with the bodily fluid source (e.g., a combined volume of a lumen of a needle, the inlet  113 , and at least a portion of the fluid flow path  115 ). 
     The outlet  114  of the housing  110  is in fluid communication with and/or is configured to be placed in fluid communication with the fluid flow paths  115  and/or  116 . The outlet  114  can be any suitable outlet, opening, port, stopcock, lock (e.g., a luer lock), seal, coupler, valve (e.g. one-way, check valve, duckbill valve, umbrella valve, and/or the like), etc. and is configured to be physically and/or fluidically coupled to the fluid collection device  180 . In some embodiments, the outlet  114  can be monolithically formed with the fluid collection device  180 . In other embodiments, the outlet  114  can be at least temporarily coupled to the fluid collection device  180  via an adhesive, a resistance fit, a mechanical fastener, a threaded coupling, a piercing or puncturing arrangement, a number of mating recesses, and/or any other suitable coupling or combination thereof. In still other embodiments, the outlet  114  can be operably coupled to the fluid collection device  180  via an intervening structure (not shown in  FIG. 1 ), such as sterile tubing and/or the like. In some embodiments, the arrangement of the outlet  114  can be such that the outlet  114  is physically and/or fluidically sealed prior to coupling to the fluid collection device  180 . In some embodiments, the outlet  114  can be transitioned from a sealed configuration to an unsealed configuration in response to being coupled to the fluid collection device  180  and/or in response to a negative pressure differential between an environment within the outlet  114  and/or housing  110  and an environment within the fluid collection device  180 . 
     Although the outlet  114  of the control device  100  and/or the housing  110  is described above as being fluidically coupled to and/or otherwise placed in fluid communication with the fluid collection device  180 , in other embodiments, the device  100  can be used in conjunction with any suitable bodily fluid collection device, system, adapter, and/or the like. For example, in some embodiments, the device  100  can be used in or with any suitable fluid transfer device and/or adapter such as those described in U.S. Pat. No. 10,123,783 entitled, “Apparatus and Methods for Disinfection of a Specimen Container,” filed Mar. 3, 2015 (referred to herein as “the &#39;783 patent”) and/or U.S. Patent Publication No. 2015/0342510 entitled, “Sterile Bodily-Fluid Collection Device and Methods,” filed Jun. 2, 2015 (referred to herein as “the &#39;510 publication”), the disclosure of each of which is incorporated herein by reference in its entirety. 
     The fluid collection device  180  can be any suitable device for at least temporarily containing a bodily fluid, such as, for example, any of those described in detail above (e.g., an evacuated container, a sample reservoir, a syringe, a culture bottle, etc.). In some embodiments, the fluid collection device  180  can be a sample reservoir that includes a vacuum seal that maintains negative pressure conditions (vacuum conditions) inside the sample reservoir, which in turn, can facilitate withdrawal of bodily fluid from the patient, through the control device  100 , and into the sample reservoir, via a vacuum or suction force. In embodiments in which the fluid collection device  180  is an evacuated container or the like, the user can couple the fluid collection device  180  to the outlet  114  to initiate a flow of bodily fluid from the patient and into the device  100  such that a first or initial portion of the flow of bodily fluid is transferred into and sequestered by the sequestration chamber  130 , and a second or subsequent portion of the flow of bodily fluid bypasses and/or is otherwise diverted away from the sequestration chamber  130  and into the fluid collection device  180  (e.g., via the outlet  114 ), as described in further detail herein. 
     The flow controller  140  of the device  100  is at least partially disposed within the housing  110  and is configured to control, direct, and/or otherwise facilitate a selective flow of fluid through at least a portion of the housing  110 . More particularly, in some embodiments, the flow controller  140  can be disposed within and/or can at least partially define a portion of the sequestration chamber  130  and/or an inner volume of the sequestration chamber  130  that receives the initial flow or amount of bodily fluid. In some embodiments, the flow controller  140  can be disposed within the housing  110  such that one or more surfaces of the flow controller  140  and one or more inner surfaces of the housing  110  collectively define the sequestration chamber  130 . Said another way, the flow controller  140  can be disposed within the sequestration chamber  130  such that an inner surface of the housing  110  at least partially defining the sequestration chamber  130  and one or more surfaces of the flow controller  140  collectively define a portion of the sequestration portion  130  and/or a volume within the sequestration chamber  130 . In some embodiments, the flow controller  140  can form a barrier and/or otherwise can fluidically isolate at least a portion of the fluid flow path  115  from at least a portion of the fluid flow path  116 . For example, the flow controller  140  can be disposed in the housing  110  such that a first side and/or surface of the flow controller  140  is selectively in fluid communication with the at least a portion of the fluid flow path  115  and/or the inlet  113 , and a second side and/or surface of the flow controller  140  is selectively in fluid communication with at least a portion of the fluid flow path  116  and/or the outlet  114 . 
     The flow controller  140  can be any suitable shape, size, and/or configuration. For example, the flow controller  140  can be, for example, a membrane, a diaphragm, a bladder, a plunger, a piston, a bag, a pouch, and/or any other suitable member having a desired stiffness, flexibility, and/or durometer. In some embodiments, the flow controller  140  can be configured to transition from a first state to a second state in response to a negative pressure differential and/or suction force exerted on at least a portion of the flow controller  140 . For example, in some embodiments, the flow controller  140  can be a bladder configured to transition or “flip” from a first state to a second state in response to a negative pressure differential and/or suction force exerted on a surface of the bladder, as described in further detail herein with reference to specific embodiments. 
     The flow controller  140  can be in a first state prior to using the device  100  (e.g., a storage or non-use state) and in response to the outlet  114  be fluidically coupled to the fluid collection device  180  (e.g., a collection device defining or configured to define a negative pressure and/or suction force), the flow controller  140  can be transitioned to a second state. In some embodiments, the flow controller  140  can define at least a portion of the sequestration chamber  130  when the flow controller  140  is in the second state. In some embodiments, the arrangement of the flow controller  140  is such that the sequestration chamber  130  defines and/or has a first volume when the flow controller  140  is in the first state and a second volume, greater than the first volume, when the flow controller  140  is placed in the second state. As described in further detail herein, the increase in the volume of the sequestration chamber  130  can result in a suction force operable to draw the initial volume of bodily fluid into the sequestration chamber  130 . Moreover, in some embodiments, the flow controller  140  can have a size, shape, and/or configuration that allows the sequestration chamber  130  to receive a volume of air or gas (e.g., a volume of air disposed in the flow path between the bodily fluid source and the sequestration portion) and the initial amount or volume of bodily fluid. In such embodiments, the flow controller  140  can be configured to define any number of portions, volumes, channels, etc., that can receive and/or contain at least one of a volume of air or the initial volume of bodily fluid. 
     In some embodiments, a size, shape, arrangement, and/or constituent material of the flow controller  140  can be configured and/or otherwise selected such that the flow controller  140  transitions from the first state to the second state in a predetermined manner and/or with a predetermined or desired rate. In some instances, controlling a rate at which the flow controller  140  transitions from the first state to the second state can, in turn, control and/or modulate a rate of bodily fluid flow into the sequestration chamber  130  and/or a magnitude of a suction force generated in the sequestration chamber  130  that is operable in drawing the initial volume of bodily fluid into the sequestration chamber  130 . Although not shown in  FIG. 1 , in some embodiments, the housing  110  can include a valve, a membrane, a porous material, a restrictor, an orifice, and/or any other suitable member, device, and/or feature configured to modulate a suction force exerted on a surface of the flow controller  140 , which in turn, can modulate the rate at which the flow controller  140  transitions from the first state to the second state. 
     In some instances, controlling a rate at which the flow controller  140  transitions and/or a magnitude of a pressure differential and/or suction force generated within the sequestration chamber  130  can reduce, for example, hemolysis of a blood sample and/or a likelihood of collapsing a vein (e.g., which is particularly important when procuring bodily fluid samples from fragile patients). In some instances, modulating the transitioning of the flow controller  140  and/or the pressure differential generated in the sequestration chamber  130  can at least partially control an amount or volume of bodily fluid transferred into the sequestration chamber  130  (i.e., can control a volume of the initial amount of bodily fluid). 
     The actuator  150  of the device  100  is at least partially disposed within the housing  110  and is configured to control, direct, and/or otherwise facilitate a selective flow of fluid through at least a portion of the housing  110 . The actuator  150  can be any suitable shape, size, and/or configuration. For example, in some embodiments, the actuator  150  can be any suitable member or device configured to transition between a first state and a second state. In some embodiments, for example, the actuator  150  can be a valve, plunger, seal, membrane, bladder, flap, plate, rod, switch, and/or the like. In some embodiments, the actuator  150  can include one or more seals configured to selectively establish fluid communication between the fluid flow channels  113  and  116  when the actuator  150  is transitioned from a first state to a second state. 
     The actuator  150  can be actuated and/or transitioned between the first state and the second state in any suitable manner. For example, in some embodiments, transitioning the actuator  150  can include activating, pressing, moving, translating, rotating, switching, sliding, opening, closing, and/or otherwise reconfiguring the actuator  150 . In some instances, the actuator  150  can transition between the first and the second state in response to a manual actuation by the user (e.g., manually exerting a force on a button, slider, plunger, switch, valve, rotational member, conduit, etc.). In other embodiments, the actuator  150  can be configured to automatically transition between the first state and the second state in response to a pressure differential (or lack thereof), a change in potential or kinetic energy, a change in composition or configuration (e.g., a portion of an actuator could at least partially dissolve or transform), and/or the like. In still other embodiments, the actuator  150  can be mechanically and/or electrically actuated or transitioned (e.g., via a motor and/or the like) based on a predetermined time, volume of bodily fluid received, volumetric flow rate of a flow of bodily fluid, flow velocity of a flow of bodily fluid, etc. While examples of actuators and/or ways in which an actuator can transition are provided, it should be understood that they have been presented by way of example only and not limitation. 
     In some embodiments, the actuator  150  can be configured to isolate, sequester, separate, and/or otherwise prevent fluid communication between at least a portion of the fluid flow path  115  and at least a portion of the fluid flow path  116  when in the first state and can be configured to place the fluid flow path  115  (or at least a portion thereof) in fluid communication with the fluid flow path  116  (or at least a portion thereof) when in the second state. In addition, the actuator  150  can be configured to sequester, separate, isolate, and/or otherwise prevent fluid communication between the sequestration chamber  130  and the inlet  113 , the outlet  114 , and/or at least a portion of the fluid flow paths  115  and  116 . Accordingly, when the actuator  150  is placed in its second state, the sequestration chamber  130  can be sequestered and/or fluidically isolated from other flow paths or portions of the housing  110  and the inlet  113  can be placed in fluid communication with the outlet  114 . As such, the actuator  150  can allow a subsequent volume of bodily fluid (e.g., a volume of bodily fluid after the initial volume of bodily fluid) to be transferred to the fluid collection device  180  fluidically coupled to the outlet  114 , as described in further detail herein. 
     As described above, the device  100  can be used to procure a bodily fluid sample having reduced contamination from microbes such as, for example, dermally residing microbes, and/or the like. For example, in some instances, a user such as a doctor, physician, nurse, phlebotomist, technician, etc. can manipulate the device  100  to establish fluid communication between the inlet  113  and the bodily fluid source (e.g., a vein of a patient, cerebral spinal fluid (CSF) from the spinal cavity, urine collection, and/or the like). As a specific example, in some instances, the inlet  113  can be coupled to and/or can include a needle or the like that can be manipulated to puncture the skin of the patient and to insert at least a portion of the needle in the vein of the patient, thereby placing the inlet  113  in fluid communication with the bodily fluid source (e.g., the vein, an IV catheter, a PICC, etc.). 
     In some embodiments, once the inlet  113  is placed in fluid communication with the bodily fluid source (e.g., the portion of the patient), the outlet  114  can be fluidically coupled to the fluid collection device  180 . As described above, in some embodiments, the fluid collection device  180  can be any suitable reservoir, container, and/or device configured to receive a volume of bodily fluid. For example, the fluid collection device  180  can be an evacuated reservoir or container that defines a negative pressure and/or can be a syringe that can be manipulated to produce a negative pressure. In some instances, coupling the outlet  114  to the fluid collection device  180  selectively exposes at least a portion of the fluid flow path  116  to the negative pressure and/or suction force within the fluid collection device  180 . As described above, a portion and/or surface of the flow controller  140  can be in fluid communication with the fluid flow path  116  and, as such, the negative pressure and/or suction force can be exerted on the portion and/or surface of the flow controller  140 . The negative pressure and/or suction force, in turn, can be operable to transition the flow controller  140  from its first state, in which the sequestration chamber  130  has the first volume, to its second state, in which the sequestration chamber  130  has the second volume, greater than the first volume. As such, an initial volume of bodily fluid can be drawn into the sequestration chamber  130  in response to the transitioning of the flow controller  140  (e.g., the increase in volume of the sequestration chamber  130  as a result of the flow controller  140  transitioning from the first state to the second state). 
     In some embodiments, for example, the flow controller  140  can be a bladder or the like configured to transition or “flip” in response to the negative pressure. The flow controller  140  can be configured to transition in a predetermined manner and/or with a predetermined rate, which in turn, can control, modulate, and/or otherwise determine one or more characteristics associated with a flow of an initial volume of bodily fluid into the sequestration chamber  130 . In some embodiments, the flow controller  140  and, for example, one or more inner surfaces of the housing  110  can collective define a number of different portions of the sequestration chamber  130 . In such embodiments, at least one of the portions of the sequestration chamber  130  can be configured to contain a volume of air that was drawn into the sequestration chamber  130  immediately before the initial volume of bodily fluid, as described in detail above. Thus, the transitioning of the flow controller  140  from the first state to the second state can result in the initial portion of the volume of bodily fluid (also referred to herein as an “initial volume” or a “first volume”) flowing from the inlet  113 , through at least a portion of the fluid flow path  115 , and into the sequestration chamber  130 . In some embodiments, transitioning the flow controller  140  from the first state to the second state can transition the control device  100  from a first or initial state or configuration to a second state or configuration in which the initial portion or volume of bodily fluid can flow in or through at least a portion the fluid flow path  115  and into the sequestration chamber  130 . 
     The initial volume of bodily fluid can be any suitable volume of bodily fluid, as described above. For example, in some instances, the control device  100  can remain in the second state or configuration until a predetermined and/or desired volume (e.g., the initial volume) of bodily fluid is transferred to the sequestration chamber  130 . In some embodiments, the initial volume can be associated with and/or at least partially based on a volume of the sequestration chamber  130  or a portion thereof (e.g., a volume sufficient to fill the sequestration chamber  130  or a desired portion of the sequestration chamber  130 ). In other embodiments, the initial volume of bodily fluid can be associated with and/or at least partially based on an amount or volume of bodily fluid that is equal to or greater than a volume associated with the fluid flow path defined between the bodily fluid source and the sequestration chamber  130 . In still other embodiments, the control device  100  can be configured to transfer a flow of bodily fluid (e.g., the initial volume) into the sequestration chamber  130  until a pressure differential between the sequestration chamber  130  and the fluid flow path  115  and/or the bodily fluid source is brought into substantial equilibrium and/or is otherwise reduced below a desired threshold. 
     After the initial volume of bodily fluid is transferred and/or diverted into the sequestration chamber  130 , the control device  100  can be transitioned from the second state or configuration to a third state or configuration. For example, in some embodiments, the actuator  150  can be transitioned from its first state to its second state when the initial volume of bodily fluid is transferred into the sequestration chamber  130 , which in turn, places the control device  100  in its third state. More particularly, in some embodiments, the arrangement of the control device  100  and/or the sequestration chamber  130  can be such that a flow of bodily fluid into the sequestration chamber  130  substantially stops or slows in response to receiving the initial volume. In some embodiments, for example, the sequestration chamber  130  can receive the flow of bodily fluid (e.g., the initial volume of bodily fluid) until a pressure differential equalizes within the sequestration chamber  130  and/or between the sequestration chamber  130  and the fluid flow path  115  and/or the bodily fluid source. In some instances, the user can visually inspect a portion of the device  100  and/or housing  110  to determine that the initial volume of bodily fluid is disposed in the sequestration chamber  130  and/or that the flow of bodily fluid into the sequestration chamber  130  has slowed or substantially stopped. In some embodiments, the user can exert a force on the actuator  150  and/or can otherwise actuate the actuator  150  to transition the actuator  150  from its first state to its second state. In other embodiments, the actuator  150  can be transitioned automatically (e.g., without user intervention). 
     The transitioning of the actuator  150  from its first state to its second state (e.g., placing the control device  100  in its third state or configuration) can sequester, isolate, separate, and/or retain the initial volume of the bodily fluid in the sequestration chamber  130 . As described in further detail herein, in some instances, contaminants such as, for example, dermally residing microbes or the like dislodged during the venipuncture event, other external sources of contamination, colonization of catheters and PICC lines that are used to collect samples, and/or the like can be entrained and/or included in the initial volume of the bodily fluid. Thus, such contaminants are sequestered in the sequestration chamber  130  when the initial volume is sequestered therein. 
     In addition to sequestering the initial volume of bodily fluid in the sequestration chamber  130 , placing the actuator  150  in its second state can also establish fluid communication between at least a portion of the fluid flow paths  115  and  116  such that a subsequent volume(s) of bodily fluid can flow through at least a portion the fluid flow paths  115  and/or  116  from the inlet  113  to the outlet  114 . For example, in some embodiments, transitioning the actuator  150  from its first state to its second state can, for example, open or close a port or valve, move one or more seals, move or remove one or more obstructions, define one or more portions of a flow path, and/or the like. With the fluid collection device  180  fluidically coupled to the outlet  114  and with the control device  100  being in the third state or configuration, the negative pressure differential and/or the suction force otherwise exerted on the flow controller  140  can be exerted on or through at least a portion of the fluid flow paths  115  and  116 . Thus, any subsequent volume(s) of the bodily fluid can flow from the inlet  113 , through at least a portion of the fluid flow paths  115  and  116 , through the outlet  114 , and into the fluid collection device  180 . As described above, sequestering the initial volume of bodily fluid in the sequestration chamber  130  prior to collecting or procuring one or more sample volumes of bodily fluid (e.g., in the fluid collection device  180 ) reduces and/or substantially eliminates an amount of contaminants in the one or more sample volumes. Moreover, in some embodiments, the arrangement of the control device  100  can be such that the control device  100  cannot transition to the third state prior to collecting and sequestering the initial volume in the sequestration chamber  130 . 
       FIGS. 2-11  illustrate a fluid control device  200  according to another embodiment. The fluid control device  200  (also referred to herein as “control device” or “device”) can be similar in at least form and/or function to the device  100  described above with reference to  FIG. 1 . For example, as described above with reference to the device  100 , in response to being placed in fluid communication with a negative pressure source (e.g., a suction or vacuum source), the device  200  can be configured to (1) withdraw bodily fluid from a bodily fluid source into the device  200 , (2) divert and sequester a first portion or amount (e.g., an initial volume) of the bodily fluid in a portion of the device  200 , and (3) allow a second portion or amount (e.g., a subsequent volume) of the bodily fluid to flow through the device  200 —bypassing the sequestered initial volume—and into a fluid collection device fluidically coupled to the device  200 . As such, contaminants or the like can be sequestered in or with the initial volume of bodily fluid, leaving the subsequent volume of bodily fluid substantially free of contaminants. 
     The fluid control device  200  (also referred to herein as “control device” or “device”) includes a housing  210 , a flow controller  240 , and an actuator  250 . In some embodiments, the control device  200  or at least a portion of the control device  200  can be arranged in a modular configuration in which one or more portions of the housing  210  and/or the actuator  250  can be physically and fluidically coupled (e.g., by an end user) to collectively form the control device  200 . Similarly, in some embodiments, the control device  200  can be packaged, shipped, and/or stored independent of a fluid collection device (e.g., a sample reservoir, syringe, etc.) and/or an inlet device (e.g., a needle, catheter, peripheral intravenous line (M), peripherally inserted central catheter (PICC), etc.), which a user can couple to the control device  200  before or during use. In other embodiments, the control device  200  need not be modular. For example, in some embodiments, the control device  200  can be assembled during manufacturing and delivered to a supplier and/or end user as an assembled device. In some embodiments, the control device  200  can include and/or can be pre-coupled (e.g., during manufacturing and/or prior to being delivered to an end user) to a fluid collection device such as any of those described above. Similarly, in some embodiments, the control device  200  can include and/or can be pre-coupled to an inlet device such as any of those described herein. 
     The housing  210  of the control device  200  can be any suitable shape, size, and/or configuration. The housing  210  includes an actuator portion  212  and a sequestration portion  220 . The actuator portion  212  of the housing  210  receives at least a portion of the actuator  250 . The sequestration portion  220  of the housing  210  is coupled to a cover  235  and includes, receives, houses, and/or at least partially defines a sequestration chamber  230 . As described in further detail herein, the housing  210  can include and/or can define a first port  217  and a second port  218 , each of which establishes fluid communication between the actuator portion  212  and the sequestration portion  220  of the housing  210  to selectively control and/or allow a flow of fluid through one or more portions of the housing  210 . 
     As shown in  FIGS. 2-6 , the actuator portion  212  of the housing  210  includes an inlet  213  and an outlet  214 . The inlet  213  is configured to be placed in fluid communication with a bodily fluid source to receive a flow of bodily fluid therefrom, as described in detail above. For example, the inlet  213  can be coupled directly or indirectly to a lumen-containing device such as a needle, IV catheter, PICC line, and/or the like, which in turn, is in fluid communication with the bodily fluid source (e.g., inserted into a patient). The outlet  214  is configured to be fluidically coupled to a fluid collection device such as any of those described above. For example, the fluid collection device can be a sample reservoir, a syringe, an intermediary bodily fluid transfer device, adapter, or vessel (e.g., a transfer adapter similar to those described in the &#39;783 patent), and/or the like. Moreover, the fluid collection device can define and/or can be manipulated to define a vacuum within the fluid collection device such that coupling the fluid collection device to the outlet  214  generates a negative pressure differential between one or more portions of the housing  210 , as described in further detail herein. 
     As shown, for example, in  FIGS. 7-11 , the actuator portion  212  defines a fluid flow path  215  in fluid communication with the inlet  213  and a fluid flow path  216  in fluid communication with the outlet  214 . More particularly, the fluid flow path  215  (e.g., a first fluid flow path) is configured to selectively place the inlet  213  in fluid communication with the first port  217  and the fluid flow path  216  (e.g., a second fluid flow path) is configured to selectively place the outlet  214  in fluid communication with the second port  218 . In addition, after an initial volume of bodily fluid has been transferred into the sequestration chamber  230 , fluid communication can be established between the fluid flow paths  215  and  216 , thereby allowing a subsequent volume of bodily fluid to flow from the inlet  213 , through at least a portion of the fluid flow paths  215  and  216 , and to the outlet  214  (and/or to a fluid collection device coupled to the outlet  214 ), as described in further detail herein. 
     The sequestration portion  220  of the housing  210  can be any suitable shape, size, and/or configuration. As shown, for example, in  FIGS. 6-8 , the sequestration portion  220  includes and/or forms an inner surface, a portion of which is arranged and/or configured to form a first contoured surface  221 . At least a portion of the first contoured surface  221  can form and/or define a portion of the sequestration chamber  230 , as described in further detail herein. Furthermore, the first port  217  and the second port  218  are configured to form and/or extend through a portion of the first contoured surface  221  to selectively place the sequestration chamber  230  in fluid communication with the fluid flow paths  215  and  216 , as described in further detail here. 
     The sequestration portion  220  is configured to include, form, and/or house, a contour member  225  and the flow controller  240 . More particularly, as shown in  FIGS. 6-8 , the sequestration portion  220  receives and/or is coupled to the contour member  225  such that the flow controller  240  is disposed therebetween. In some embodiments, the contour member  225  can be fixedly coupled to the sequestration portion  220  via an adhesive, ultrasonic welding, and/or any other suitable coupling method. In some embodiments, the contour member  225 , the sequestration portion  220 , and the flow controller  240  can collectively form a substantially fluid tight and/or hermetic seal that isolates the sequestration portion  220  from a volume outside of the sequestration portion  220 . 
     As shown, a cover  235  is configured to be disposed about the contour member  225  such that the cover  235  and the sequestration portion  220  of the housing  210  enclose and/or house the contour member  225  and the flow controller  240 . In some embodiments, the cover  235  can be coupled to the contour member  225  and/or the sequestration portion  220  via an adhesive, ultrasonic welding, one or more mechanical fasteners, a friction fit, a snap fit, a threaded coupling, and/or any other suitable manner of coupling. In some embodiments, the cover  235  can define an opening, window, slot, etc. configured to allow visualization of at least a portion of the sequestration chamber  230 . While the contour member  225  and the cover  235  are described above as being separate pieces and/or components, in other embodiments, the contour member  225  can be integrated and/or monolithically formed with the cover  235 . 
     The contour member  225  includes and/or forms a second contoured surface  226 . The arrangement of the contour member  225  and the sequestration portion  220  of the housing  210  can be such that at least a portion of the first contoured surface  221  is aligned with and/or opposite a corresponding portion of the second contoured surface  226  of the contour member  225  (see e.g.,  FIG. 8 ). As such, a space, volume, opening, void, chamber, and/or the like defined between the first contoured surface  221  and the second contoured surface  226  forms and/or defines the sequestration chamber  230 . Moreover, the flow controller  240  is disposed between the first contoured surface  221  and the second contoured surface  226  and can be configured to transition between a first state and a second state in response to a negative pressure differential and/or suction force applied to at least a portion of the sequestration chamber  230 , as described in further detail herein. 
     The ports  217  and  218  of the housing  210  can be any suitable shape, size, and/or configuration. As described above, the first port  217  is in fluid communication with the sequestration chamber  230  and can selectively establish fluid communication between the sequestration chamber  230  and the fluid flow path  215  and/or the inlet  213 . More specifically, the first port  217  is in fluid communication with a first portion of the sequestration chamber  230  defined between the second contoured surface  226  and a first side of the flow controller  240 . As described in further detail herein, the first port  217  can be configured to provide and/or transfer a flow of bodily fluid from the inlet  213  and the fluid flow path  215  and into the first portion of the sequestration chamber  230  defined between the second contoured surface  226  and the first side of the flow controller  240  in response to the flow controller  240  transitioning from a first state to a second state. 
     The second port  218  is in fluid communication with the sequestration chamber  230  and can selectively establish fluid communication between the sequestration chamber  230  and the fluid flow path  216  and/or the outlet  214 . More specifically, the second port  218  is in fluid communication with a second portion of the sequestration chamber  230  defined between the first contoured surface  221  and a second side of the flow controller  240  (e.g., opposite the first side). As described in further detail herein, the second port  218  can be configured to expose the second portion of the sequestration chamber  230  defined between the first contoured surface  221  and the second side of the flow controller  240  to a negative pressure differential and/or suction force resulting from the fluid collection device (e.g., an evacuated container, a culture bottle, a syringe, and/or the like) being fluidically coupled to the outlet  214 . In turn, the negative pressure differential and/or suction force can be operable to transition the flow controller  240  from its first state to its second state. In some instances, it may be desirable to modulate and/or control a magnitude of the negative pressure differential. As such, the second port  218  can include and/or can be coupled to a restrictor  219 . The restrictor  219  can be configured to limit and/or restrict a flow of fluid (e.g., air or gas) between the second portion of the sequestration chamber  230  and the fluid flow path  216 , thereby modulating and/or controlling a magnitude of a pressure differential and/or suction force applied on or experienced by the flow controller  240 , as described in further detail herein. 
     The flow controller  240  is disposed within the housing  210  between the sequestration portion  220  and the contour member  225  (e.g., within the sequestration chamber  230 ). The flow controller  240  can be any suitable shape, size, and/or configuration. Similarly, the flow controller  240  can be formed of any suitable material (e.g., any suitable biocompatible material such as those described herein and/or any other suitable material). For example, the flow controller  240  can be a fluid impermeable bladder, membrane, diaphragm, and/or the like configured to be transitioned from a first state and/or configuration to a second state and/or configuration. In some embodiments, the flow controller  240  (e.g., bladder) can include any number of relatively thin and flexible portions configured to deform in response to a pressure differential across the flow controller  240 . For example, in some embodiments, the flow controller  240  can be formed of or from any suitable medical-grade elastomer and/or any of the biocompatible materials described above. In some embodiments, the flow controller  240  can have a durometer between about 5 Shore A and about 70 Shore A, between about 10 Shore A and about 60 Shore A, between about 20 Shore A and about 50 Shore A, between about 30 Shore A and about 40 Shore A, and/or any other suitable durometer. In some embodiments, the flow controller  240  can be formed of or from silicone having a durometer between about 20 Shore A and about 50 Shore A. More particularly, in some such embodiments, the flow controller  240  can be formed of or from silicone having a durometer of about 30 Shore A. In some embodiments, the flow controller  240  can include relatively thin and flexible portions having a thickness between about 0.001″ and about 0.1″. In other embodiments, the relatively thin and flexible portions can have a thickness that is less than 0.001″ or greater than 0.1″. 
     In some embodiments, the flow controller  240  can have a size and/or shape configured to facilitate, encourage, and/or otherwise result in fluid flow with a desired set of flow characteristics. Similarly, the flow controller  240  can be formed of or from a material having one or more material properties and/or one or more surface finishes configured to facilitate, encourage, and/or otherwise result in fluid flow with the desired set of flow characteristics. As described in further detail herein, the set of flow characteristics can be and/or can include a relatively even or smooth fluid flow, a substantially laminar fluid flow and/or a fluid flow with relatively low turbulence, a fluid flow with a substantially uniform front, a fluid flow that does not readily mix with other fluids (e.g., a flow of bodily fluid that does not mix with a flow or volume of air), and/or the like. 
     In the embodiment shown in  FIG. 2-11 , the flow controller  240  is a bladder (or diaphragm) formed of or from silicone having a durometer of about 30 Shore A. The flow controller  240  (e.g., bladder) includes a first deformable portion  241 , a second deformable portion  242 , and a third deformable portion  243 . In addition, the flow controller  240  defines an opening  244 . As shown, for example, in  FIG. 8 , the flow controller  240  can be positioned within the sequestration portion  220  of the housing  210  such that the first port  217  extends through the opening  244 . In some embodiments, the arrangement of the flow controller  240  is such that a surface of the flow controller  240  defining the opening  244  forms a substantially fluid tight seal with a portion of the inner surface of the sequestration portion  220  of the housing  210  (e.g., the portion defining and/or forming the first port  217 ). Moreover, the flow controller  240  can include one or more portions configured to form one or more seals with and/or between the flow controller  240  and each of the contoured surfaces  221  and  226 , as described in further detail herein. 
     The deformable portions  241 ,  242 , and  243  of the flow controller  240  can be relatively thin and flexible portions configured to deform in response to a pressure differential between the first side of the flow controller  240  and the second side of the flow controller  240 . More particularly, the deformable portions  241 ,  242 , and  243  can each have a thickness of about 0.005″. As shown, for example, in  FIGS. 8 and 10 , the deformable portions  241 ,  242 , and  243  of the flow controller  240  correspond to and/or have substantially the same general shape as at least a portion of the contoured surfaces  221  and/or  226 . As such, the deformable portions  241 ,  242 , and  243  and the corresponding portion of the contoured surfaces  221  and/or  226  can collectively form and/or define one or more channels or the like, which in turn, can receive the initial volume of bodily fluid, as described in further detail herein. 
     As described above, the flow controller  240  is configured to transition between a first state and a second state. For example, when the flow controller  240  is in its first state, the deformable portions  241 ,  242 , and  243  are disposed adjacent to and/or substantially in contact with the second contoured surface  226 , as shown in  FIG. 8 . More specifically, the first deformable portion  241  can be disposed adjacent to and/or substantially in contact with a first recess  227  formed by the second contoured surface  226 , the second deformable portion  242  can be disposed adjacent to and/or substantially in contact with a second recess  228  formed by the second contoured surface  226 , and the third deformable portion  243  can be disposed adjacent to and/or substantially in contact with a third recess  229  formed by the second contoured surface  226 . 
     As such, the first portion of the sequestration chamber  230  (e.g., the portion defined between the second contoured surface  226  and the first surface of the flow controller  240 ) can have a relatively small and/or relatively negligible volume. In contrast, when the flow controller  240  is transitioned from its first state to its second state (e.g., in response to a negative pressure applied and/or transmitted via the second port  218 ), at least the deformable portions  241 ,  242 , and  243  are disposed adjacent to and/or substantially in contact with the first contoured surface  221 . More specifically, the first deformable portion  241  can be disposed adjacent to and/or substantially in contact with a first recess  222  formed by the first contoured surface  221 , the second deformable portion  242  can be disposed adjacent to and/or substantially in contact with a second recess  223  formed by the first contoured surface  221 , and the third deformable portion  243  can be disposed adjacent to and/or substantially in contact with, for example, a non-recessed portion of the first contoured surface  221 . 
     Accordingly, a volume of the first portion of the sequestration chamber  230  is larger when the flow controller  240  is in its second state than when the flow controller is in its first state. In other words, the deformable portions  241 ,  242 , and  243  and the second contoured surface  226  can define one or more channels (e.g., the sequestration chamber  230 ) configured to receive the initial volume of bodily fluid. In some instances, the increase in the volume of the first portion of the sequestration chamber  230  can result in a negative pressure or vacuum therein that can be operable to draw the initial volume of bodily fluid into the sequestration chamber  230 , as described in further detail herein. Moreover, in some embodiments, the arrangement of deformable portions  241 ,  242 , and/or  243  can be such that a volume of air drawn into the sequestration chamber  230  immediately before the flow of bodily fluid can flow into and/or be disposed in a portion of the sequestration chamber  230  corresponding to the first deformable portion  241  and/or the second deformable portion  242 . 
     While the flow controller  240  is particularly described above with reference to  FIGS. 6-11 , in other embodiments, the flow controller  240  and/or the sequestration chamber  230  can have any suitable configuration and/or arrangement. For example, in some embodiments, the contoured surfaces  221  and/or  226  can include more or fewer recesses (e.g., the recesses  222  and  223  and the recesses  227 ,  228 , and  229 , respectively). In other embodiments, a depth of one or more recesses can be modified. Similarly, the flow controller  240  can be modified in any suitable manner to substantially correspond to a shape and/or configuration of the contoured surfaces  221  and/or  226 . In some embodiments, such modifications can, for example, modify one or more characteristics associated with a flow of a gas (e.g., air) and/or fluid (e.g., bodily fluid), one or more characteristics associated with the manner or rate at which the flow controller  240  transitions, and/or the like, as described in further detail herein. 
     While the flow controller  240  is described as being a bladder or the like including a number of deformable portions, in other embodiments, a flow controller can be arranged and/or configured as, for example, a bellows, a flexible pouch, an expandable bag, an expandable chamber, a plunger (e.g., similar to a syringe), and/or any other suitable reconfigurable container or the like. In addition, the sequestration chamber  230  at least partially formed by the flow controller  240  can have any suitable shape, size, and/or configuration. 
     The actuator  250  of the control device  200  can be any suitable shape, size, and/or configuration. At least a portion of the actuator  250  is disposed within the actuator portion  212  of the housing  210  and is configured to be transitioned between a first state, configuration, and/or position and a second state, configuration, and/or position. In the embodiment shown in  FIGS. 2-11 , the actuator  250  is configured as an actuator rod or plunger configured to be moved relative to the actuator portion  212  of the housing  210 . The actuator  250  includes an end portion  251  disposed outside of the housing  210  and configured to be engaged by a user to transition the actuator  250  between its first state and its second state. As shown in  FIGS. 6-11 , a portion of the actuator  250  includes and/or is coupled to a set of seals  255 . The seals  255  can be, for example, o-rings, elastomeric over-molds, proud or raised dimensions or fittings, and/or the like. The arrangement of the actuator  250  and the actuator portion  212  of the housing  210  can be such that an inner portion of the seals  255  forms a fluid tight seal with a surface of the actuator  250  and an outer portion of the seals  255  forms a fluid tight seal with an inner surface of the actuator portion  212  of the body  210 . In other words, the seals  255  form one or more fluid tight seals between the actuator  250  and the inner surface of the actuator portion  212 . As shown in  7 - 11 , the actuator  250  includes and/or is coupled to four seals  255  which can be distributed along the actuator  250  to selectively form and/or define one or more flow paths therebetween. Moreover, the actuator  250  defines a flow channel  252  defined between a pair of seals  255  which can aid and/or facilitate the fluid communication between the fluid flow paths  215  and  216  when the actuator  250  is transitioned to its second state, as described in further detail herein. While the actuator  250  is described above as including four seals  255 , in other embodiments, the actuator  250  can include fewer than four seals  255  or more than four seals  255 . 
     In some embodiments, the actuator portion  212  of the housing  210  and the actuator  250  collectively include and/or collectively form a lock. For example, as shown in  FIGS. 6 and 8 , the actuator portion  212  of the housing  210  can define an opening  238  and the actuator  250  can include a locking member, latch, protrusion, tab, and/or the like (referred to herein as “lock  253 ”) configured to be disposed, at least partially, within the opening  238 . In some embodiments, the lock  253  can be arranged and/or disposed in the opening  238  and can limit and/or substantially prevent the actuator  250  from being removed from the housing  210 . In some embodiments, the lock  253  can be transitioned between a locked state, in which the lock  253  limits and/or substantially prevents the actuator  250  from being moved relative to the housing  210 , and an unlocked state, in which the actuator  250  can be moved, for example, between its first state and/or position and its second state and/or position. In some instances, such an arrangement may limit and/or substantially prevent the actuator  250  from being actuated, for example, prior to transferring the initial volume of bodily fluid in the sequestration chamber  230 . In other embodiments, the lock  253  can transition from the unlocked state to a locked state, for example, after transferring the initial volume of bodily fluid into the sequestration chamber  230 . 
     As shown in  FIGS. 7 and 8 , when the actuator  250  is disposed in its first state and/or position (e.g., prior to using the device  100 ), the fluid flow path  215  can establish fluid communication between the inlet  213  and the first port  217 . More particularly, the actuator  250  can be in a position relative to the housing  210  such that each of the seals  255  is disposed on a side of the inlet  213  opposite to a side of the inlet  213  associated with the first port  217 . In other words, the actuator  250  and/or the seals  255  do not obstruct and/or occlude the fluid flow path  215  when the actuator  250  is in the first state and/or position, as shown in  FIGS. 7 and 8 . As such, when the actuator  250  is in the first state and/or position, a volume of bodily fluid (e.g., an initial volume) can flow from the inlet  213 , through the fluid flow path  215  and the first port  217 , and into the sequestration chamber  230 , as described in further detail herein. 
     As shown in  FIGS. 9-11 , a force can be exerted on the end portion  251  of the actuator  250  to place the actuator  250  in its second state and/or position. When in the second state and/or position, the inlet  213  and the outlet  214  are placed in fluid communication via at least a portion of the fluid flow paths  215  and  216  and/or the flow channel  252 . As shown in  FIGS. 9 and 11 , the actuator  250  can be position such that the inlet  213  and the outlet  214  are each disposed between the same pair of seals  255 , thereby allowing a flow of bodily fluid therethrough. In addition, the flow channel  252  defined by the actuator  250  assists and/or facilitates the flow of bodily fluid (see e.g.,  FIG. 11 ). For example, in some embodiments, the flow channel  252  can establish fluid communication between a portion of the fluid flow path  215  defined by the inlet  213  and a portion of the fluid flow path  216  defined by the outlet  214 . Moreover, the arrangement of the seals  255  is such that the first port  217  and the second port  218  are each sequestered and/or isolated from each of the inlet  213  and the outlet  214 . As such, placing the actuator  250  in the second state and/or position can (1) sequester and/or isolate the sequestration chamber  230  and any volume of bodily fluid disposed therein and (2) establish fluid communication between the inlet  213  and the outlet  214 , thereby allowing a volume of bodily fluid to flow through the device  200  and into a fluid collection device (not shown) fluidically coupled to the outlet  214 . 
     In some embodiments, the set of seals  255  can be configured to sequester, isolate, and/or seal one or more portions of the device  200  prior to establishing fluid communication between other portions of the device  200 . For example, in some embodiments, the actuator  250  can be in a first position relative to the actuator portion  212  of the housing  210  when in the first state, as described above. In such instances, actuating the actuator  250  (e.g., exerting a force of the end portion  251  of the actuator  250 ) can include moving the actuator  250  from the first position relative to the actuator portion  212  to a second position relative to the actuator portion  212 , in which (1) a first seal  255  is disposed between the first port  217  and the inlet  213  and/or a lumen thereof, (2) the inlet  213  and/or the lumen thereof is disposed between the first seal  255  and a second seal  255 , (3) the outlet  214  and/or a lumen thereof is disposed between the second seal  255  and a third seal  255 , and (4) the second port  218  is disposed between the third seal  255  and a fourth seal  255 . In this manner, the inlet  213  is sequestered from the first port  217 , the outlet  214  is sequestered from the second port  218 , and fluid communication has not yet been established between the inlet  213  and the outlet  214  (e.g., the inlet  213  is sequestered from the outlet  214 ). 
     In some instances, actuating the actuator  250  can further include moving the actuator  250  from the second position relative to the actuator portion  212  to a third position relative to the actuator portion  212 , in which the actuator  250  is in the second state. As such, the second seal  255  is disposed between the first port  217  and the inlet  213  and/or the lumen thereof, each of the inlet  213  and the outlet  214  (and/or the lumens thereof) is disposed between the second seal  255  and the third seal  255 , and the second port  218  is disposed between the third seal  255  and the fourth seal  255 . Thus, each of the first port  217  and the second port  218  are sequestered from the inlet  213  and the outlet  214  (and/or the lumens thereof), and fluid communication is established (e.g., via the flow channel  252 ) between the inlet  213  and the outlet (and/or the lumens thereof). 
     While the actuator  250 , in this example, is described as being moved between the first, second, and third positions relative to the actuator portion  212 , it should be understood that transitioning the actuator  250  from the first state to the second state can include moving the actuator  250  in a substantially continuous manner from the first position relative to the actuator portion  212 , through the second position relative to the actuator portion  212 , and to the third position relative to the actuator portion  212 . In other embodiments, the actuator  250  can be actuated, moved, and/or transitioned, in any number of discrete steps. For example, in some instances, the actuator  250  can be transitioned a first predetermined amount to move the actuator  250  from the first position relative to the actuator portion  212  to the second position relative to the actuator portion  212  and can then be transitioned (e.g., in a second and/or discrete step) a second predetermined amount to move the actuator  250  from the second position relative to the actuator portion  212  to the third position relative to the actuator portion  212 . While the actuator  250  is described above as including four seals  255 , in other embodiments, an actuator can be functionally similar to the actuator  250  and can include fewer than four seals (e.g., one seal, two seals, or three seals) or more than four seals (e.g., five seals, six seals, seven seals, or more). 
     As described above, the device  200  can be used to procure a bodily fluid sample having reduced contamination (e.g., contamination from microbes such as, for example, dermally residing microbes, microbes external to the bodily fluid source, and/or the like). For example, prior to use, the device  200  can be in its first, initial, and/or storage state or operating mode, in which each of the flow controller  240  and the actuator  250  is in its respective first or initial state. With the device  200  in the first state, a user such as a doctor, physician, nurse, phlebotomist, technician, etc. can manipulate the device  200  to establish fluid communication between the inlet  213  and the bodily fluid source (e.g., a vein of a patient). Once the inlet  213  is placed in fluid communication with the bodily fluid source, the outlet  214  can be fluidically coupled to a fluid collection device (not shown in  FIGS. 2-11 ). In the embodiment shown in  FIGS. 2-11 , for example, the fluid collection device can be an evacuated container, a culture bottle, a sample reservoir, a syringe, and/or any other suitable container or device configured to define or produce a negative pressure, suction force, vacuum, and/or energy potential. 
     When the actuator  250  is in the first position and/or configuration, the inlet  213  of the housing  210  is in fluid communication with, for example, the fluid flow path  215 , which in turn, is in fluid communication with the first port  217 . The outlet  214  of the of the housing  210  is in fluid communication with the fluid flow path  216 , which in turn, is in fluid communication with the second port  218 . More particularly, one or more of the seals  255  of the actuator  250  can be in a position relative to the actuator portion  212  of the housing  210  that (1) allows and/or establishes fluid communication between the inlet  213 , the fluid flow path  215 , and the first port  217  and (2) fluidically isolates the inlet  213 , the fluid flow path  215 , and the first port  217  from the outlet  214 , the fluid flow path  216 , and the second port  218 , as shown in  FIGS. 7 and 8 . Thus, when the control device  200  is in the first state or operating mode (e.g., when the actuator  250  and the flow controller  240  are each in their first state), fluidically coupling the fluid collection device to the outlet  214  generates and/or otherwise results in a negative pressure differential and/or suction force within at least a portion of the fluid flow path  216  and, in turn, within the portion of the sequestration chamber  230  defined between a surface of the flow controller  240  (e.g., a first surface) and the first contoured surface  221  of the housing  210 . 
     The flow controller  240  is in the first state and/or configuration prior to the fluid collection device being coupled to the outlet  214 . In the embodiment shown in  FIGS. 2-11 , the flow controller  240  is a fluid impermeable bladder, diaphragm, membrane, and/or the like that can have a flipped, inverted, collapsed, and/or empty configuration (e.g., the first state and/or configuration) prior to coupling the fluid collection device to the outlet  214 . For example, as shown in  FIG. 8 , the flow controller  240  can be disposed adjacent to and/or in contact with the second contoured surface  226  when the flow controller  240  is in its first state and/or configuration. Said another way, the first side of the flow controller  240  (opposite the second side) can be disposed adjacent to and/or can be in contact with the second contoured surface  226 . 
     As described above, the flow controller  240  is configured to transition from its first state and/or configuration to its second state and/or configuration in response to the negative pressure differential and/or suction force generated within the portion of the sequestration chamber  230  defined between the flow controller  240  and the first contoured surface  221 . For example, the flow controller  240  can be configured to transition, move, “flip”, and/or otherwise reconfigure to its second state and/or configuration in which the flow controller  240  and/or the second side of the flow controller  240  (opposite the first side) is disposed adjacent to and/or in contact with the first contoured surface  221 , as shown in  FIG. 10 . Said another way, the negative pressure differential and/or suction force draws, pulls, and/or otherwise moves at least a portion of the flow controller  240  toward the first contoured surface  221  and away from the second contoured surface  226 . Moreover, the control device  200  is placed in its second state and/or configuration when the actuator  250  is in its first state and the flow controller  240  is in its second state. 
     The transitioning of the flow controller  240  results in an increase in an inner volume of the portion of the sequestration chamber  230  defined between a surface of the flow controller  240  (e.g., the first side of the flow controller  240 ) and the second contoured surface  226 . The increase in the inner volume can, in turn, result in a negative pressure differential between the portion of the sequestration chamber  230  (defined at least in part by the flow controller  240 ) and, for example, the inlet  213  that is operable in drawing at least a portion of an initial flow, amount, or volume of bodily fluid from the inlet  213 , through the fluid flow path  215  and the first port  217 , and into the portion of the sequestration chamber  230 . In some instances, the initial volume and/or flow of bodily fluid can be transferred into the sequestration chamber  230  until, for example, the flow controller  240  is fully expanded, flipped, and/or transitioned, until the negative pressure differential is reduced and/or equalized, and/or until a desired volume of bodily fluid is disposed within the portion of the sequestration chamber  230 . 
     In some instances, it may be desirable to modulate and/or control a manner in which the flow controller  240  is transitioned and/or a magnitude of the negative pressure differential and/or suction force generated within the sequestration chamber  230  on one or both sides of the flow controller  240 . In the embodiment shown in  FIGS. 2-11 , for example, the second port  218  defines, includes, receives, and/or is otherwise coupled to the restrictor  219  that establishes fluid communication between the fluid flow path  216  and the portion of the sequestration chamber  230  defined between the flow controller  240  and the first contoured surface  221 . 
     In some embodiments, the restrictor  219  can define a lumen or flow path having a relatively small diameter (e.g., relative to a diameter of at least a portion of the fluid flow path  216 ). For example, in some embodiments, the restrictor  219  can have a diameter of about 0.0005″, about 0.001″, about 0.003″, about 0.005″, about 0.01″, about 0.1″, about 0.5″, or more. In other embodiments, the restrictor  219  can have a diameter less than 0.0005″ or greater than 0.5″. In some embodiments, the restrictor  219  can have a predetermined and/or desired length of about 0.01″, about 0.05″, about 0.1″, about 0.15″, about 0.2″, about 0.5″, or more. In other embodiments, the restrictor  219  can have a predetermined and/or desired length that is less than 0.01″ or more than about 0.5″. Moreover, in some embodiments, the restrictor  219  can have any suitable combination of diameter and length to allow for and/or to provide a desired fluid (e.g., air) flow characteristic through at least a portion of the control device  200 . While the restrictor  219  is described above as defining a relatively small lumen and/or flow path, in other embodiments, a restrictor can have any suitable shape, size, and/or configuration. For example, in some embodiments, a restrictor can be a porous material, a semi-permeable member or membrane, a mechanical valve, float, and/or limiter, and/or any other suitable member or device configured to modulate a pressure differential across at least a portion thereof. 
     In the embodiment shown in  FIGS. 2-11 , the relatively small diameter of the restrictor  219  results in a lower magnitude of negative pressure being applied through and/or within the portion of the sequestration chamber  230  than would otherwise be applied with a restrictor have a larger diameter or if the second port  218  did not include or receive a restrictor  219 . For example, in some embodiments, a fluid collection device and/or other suitable negative pressure source may define and/or produce a negative pressure differential having a magnitude (e.g., a negative magnitude) of about 0.5 pounds per square inch (PSI), about 1.0 PSI, about 2.0 PSI, about 3.0 PSI, about 4.0 PSI, about 5.0 PSI, about 10.0 PSI, about 12.5 PSI, or about 14.7 PSI (at or substantially at atmospheric pressure at about sea level). In some embodiments, a fluid collection device such as an evacuated container or the like can have a predetermined negative pressure of about 12.0 PSI. Accordingly, by controlling the diameter and/or length of the restrictor  219 , the amount of negative pressure to which the portion of the sequestration chamber  230  is exposed and/or the rate at which the negative pressure is applied can be controlled, reduced, and/or otherwise modulated. In some instances, the use of the restrictor  219  can result in a delay or ramp up of the negative pressure exerted on or in the portion of the sequestration chamber  230 . 
     Although the pressure modulation is described above as being based on a diameter of the restrictor  219  (i.e., a single restricted flow path), it should be understood that this is presented by way of example only and not limitation. Other means of modulating the magnitude of negative pressure to which the portion of the sequestration chamber  230  is exposed can include, for example, a porous material, a valve, a membrane, a diaphragm, a specific restriction, a vent, a deformable member or flow path, and/or any other suitable means. In other embodiments, a control device can include any suitable number of restricted flow paths, each of which can have substantially the same diameter or can have varied diameters. For example, in some embodiments, a control device can include up to 100 restricted flow paths or more. In such embodiments, each of the restricted flow paths can have a diameter of between about 0.0005″ and about 0.1″, between about 0.0005″ and about 0.05″, or between about 0.0005″ and about 0.01″. In some embodiments, multiple restricted flow paths can be configured to selectively provide a flow path between the outlet  214  and the portion of the sequestration chamber  230  that exposes the portion of the sequestration chamber  230  to the negative pressure differential. 
     In some embodiments, modulating and/or controlling a magnitude of the pressure to which the portion of the sequestration chamber  230  is exposed can, in turn, modulate a rate at which one or more volumes of the sequestration chamber  230  are increased. In some instances, modulating the rate of volume increase (and thus, suction force) can modulate and/or limit a magnitude of pressure exerted on the bodily fluid and/or within a vein of a patient. In some instances, such pressure modulation can reduce, for example, hemolysis of a blood sample and/or a likelihood of collapsing a vein. In some instances, the ability to modulate and/or control an amount or magnitude of negative pressure or suction can allow the control device  200  to be used across a large spectrum of patients that may have physiological challenges whereby negative pressure is often needed to facilitate collection of bodily fluid such as, for example, blood (i.e. pressure differential between atmospheric pressure and a patient&#39;s vascular pressure is not sufficient to facilitate consistent and sufficiently forceful flow) but not so much pressure that a rapid force flattens, collapses, caves-in, and/or otherwise inhibits patency and ability to collect blood. 
     In some embodiments, the shape, size, and/or arrangement of the sequestration chamber  230  and/or the flow controller  240 , the magnitude of the negative pressure differential or suction force, and/or the way in which the negative pressure differential or suction force is exerted can dictate and/or control a rate and/or manner in which the flow controller  240  is transitioned from the first state to the second state. In some instances, controlling the rate, order, and/or manner in which the flow controller  240  is transitioned can result in one or more desired flow characteristics associated with a flow of air, gas, and/or bodily fluid into and/or through at least a portion of the sequestration chamber  230 . 
     For example, the arrangement included in this embodiment can be such that a transitioning and/or flipping of the third deformable portion  243  of the flow controller  240  is completed prior to completion of the transitioning and/or flipping of the first and second deformable portions  241  and  242 . In some instances, this arrangement can be such that a portion of the sequestration chamber  230  collectively defined by the first deformable portion  241  and the first recess  227  of the second contoured surface  226  (e.g., a first volume of the sequestration chamber  230 ) receives at least a portion of a volume of air that was within the fluid flow path between the bodily fluid source and the sequestration chamber  230  prior to the fluid flow path receiving and/or being filled with bodily fluid. Similarly, a portion of the sequestration chamber  230  collectively defined by the second deformable portion  242  and the second recess  228  of the second contoured surface  226  (e.g., a second volume of the sequestration chamber  230 ) can receive at least a portion of the volume of air that was within the fluid flow path. In other words, the transitioning of the flow controller  240  can vent, evacuate, and/or purge air or gas from the fluid flow path between the bodily fluid source and the sequestration chamber  230 , which can then be collected, stored, and/or contained within the first and second volumes of the sequestration chamber  230 . On the other hand, a portion of the sequestration chamber  230  collectively defined by the third deformable portion  243  and the third recess  229  of the second contoured surface  226  (e.g., a third volume of the sequestration chamber  230 ) can receive the initial volume of bodily fluid that flows through the fluid flow path between the bodily fluid source and the sequestration chamber  230  after the air or gas is collected in the first and/or second volumes of the sequestration chamber  230 . 
     In some instances, such an arrangement and/or order of the deformable portions  241 ,  242 , and/or  243  transitioning can result in an even flow of the initial volume of bodily fluid into, for example, the third volume of the sequestration chamber  230 . More particularly, the third deformable portion  243  is configured to complete or substantially complete the transition and/or flip from its first state and/or position prior to a complete or a substantially complete transition and/or flip of the first and/or second deformable portions  241  and/or  242 , respectively, which in turn, can allow the bodily fluid to flow into and/or through at least a portion of the third deformable portion  243  with a substantially uniform front. In this manner, the third deformable portion  243  can be in the second state, configuration, and/or position prior to the flow of bodily fluid entering the sequestration chamber  230 . Thus, the third volume of the sequestration chamber  230  can have and/or can define a relatively consistent and/or uniform cross-sectional shape and/or area as the flow of bodily fluid enters the sequestration chamber  230 , which in turn, can limit wicking of a portion of the bodily fluid flow, inconsistent local flow rates of the bodily fluid flow, and/or an otherwise uneven filling of the third volume of the sequestration chamber  230 . 
     As shown in  FIGS. 8 and 10 , the first contoured surface  221  includes the recesses  222  and  223  that are each deeper than a portion of the first contoured surface  221  aligned and/or otherwise associated with the third deformable portion  243  of the flow controller  240 . Said another way, a distance between the first recess  222  and the second recess  223  of the first contoured surface  221  and the first recess  227  and the second recess  228 , respectively, of the second contoured surface  226  is greater than a distance between the portion of the first contoured surface  221  and the third recess  229  of the second contoured surface  226 . Accordingly, a distance traveled when the first and second deformable portions  241  and  242  transition and/or flip is greater than a distance traveled when the third deformable portion  243  transitions and/or flips. Furthermore, a width of the first and second deformable portions  241  and  242  can be similar to or less than a width of the third deformable portion  243 . In some instances, such an arrangement can allow the third deformable portion  243  to complete or substantially complete its transition and/or flip prior to each of the first and second deformable portions  241  and  242 , respectively, completing or substantially completing its transition and/or flip. In other embodiments, a distance traveled and/or a width of one or more of the deformable portions  241 ,  242 , and/or  243  can be modified (increased or decreased) to modify and/or change a rate, order, and/or sequence associated with the deformable portions  241 ,  242 , and/or  243  transitioning and/or flipping from the first state to the second state. 
     In some embodiments, including fewer deformable portions or including more deformable portions can, for example, modify a relative stiffness of or associated with each deformable portion and/or can otherwise control a rate and/or manner in which each of the deformable portions transitions or flips, which in turn, can control a rate and/or manner in which fluid (e.g., air and/or bodily fluid) flows into the sequestration chamber  230 . For example, in some embodiments, increasing a number of deformable portions can result in a decrease in surface area on which the negative pressure is exerted, which in turn, can increase a pressure differential sufficient to transition and/or flip the deformable portions. While the deformable portions  241 ,  242 , and  243  are shown in  FIGS. 8 and 10  as having substantially the same thickness, in other embodiments, at least one deformable portion can have a thickness that is different from a thickness of the other deformable portions (e.g., the deformable portion  241  can have a different thickness than the thicknesses of the deformable portion  242  and/or the deformable portion  243  (or vice versa or in other combinations). In some instances, increasing a thickness of a deformable portion relative to a thickness of the other deformable portions can increase a stiffness of that deformable portion relative to a stiffness of the other deformable portions. In some such instances, the increase in the stiffness of the thicker deformable portion can, in turn, result in the other deformable portions (e.g., the thinner deformable portions) transitioning and/or flipping prior to the thicker/stiffer deformable portion transitioning and/or flipping. In some embodiments, a deformable portion can have a varied thickness along at least a portion of the deformable portion. 
     In some embodiments, a size, shape, material property, surface finish, etc. of the flow controller  240  and/or the deformable portions  241 ,  242 , and/or  243  can also facilitate, encourage, and/or otherwise result in fluid flow with the substantially uniform front. For example, the third volume of the sequestration chamber  230  (collectively defined by the third deformable portion  243  and the third recess  229  of the second contoured surface  226 ) can have a size, shape, diameter, perimeter, and/or cross-sectional area that can limit and/or substantially prevent mixing of air with the bodily fluid flow (e.g., the front of the flow) due, at least in part, to a surface tension between the flow of bodily fluid and each of the third deformable portion  243  and the third recess  229  of the second contoured surface  226 . In some embodiments, for example, the third volume of the sequestration chamber  230  can have a cross-sectional area between about 0.0001 square inch (in 2 ), and about 0.16 in 2 , between about 0.001 in 2  and about 0.08 in 2 , between about 0.006 in 2  and about 0.06 in 2 , or between about 0.025 in 2  and about 0.04 in 2 . In other embodiments, the third volume of the sequestration chamber  230  can have a cross-sectional area that is less than 0.0001 in 2  or greater than 0.16 in 2 . 
     In some embodiments, the flow controller  240  and/or the contoured member  225  (or at least the second contoured surface  226  thereof) can be formed of or from a material having one or more material properties and/or one or more surface finishes configured to facilitate, encourage, and/or otherwise result in fluid flow with the desired set of flow characteristics. In other embodiments, the flow controller  240  and/or the second contoured surface  226  can have a coating configured to result in the desired set of flow characteristics. For example, in some embodiments, the flow controller  240  and/or the second contoured surface  226  can be formed of and/or can otherwise include a coating of a hydrophobic material or a hydrophilic material. Moreover, the flow controller  240  and at least a portion of the contoured member  225  (or at least the second contoured surface  226  thereof) can be formed of or from the same material and/or can include the same coating or can be formed of or from different materials and/or can include different coatings. Similarly, the flow controller  240  and/or the second contoured surface  226  can include any suitable surface finish which can be substantially the same or different. In some instances, a non-exhaustive list of a desired set of flow characteristics can be and/or can include one or more of a relatively even or smooth fluid flow, a substantially laminar fluid flow and/or a fluid flow with relatively low turbulence, a fluid flow with a substantially uniform front, a fluid flow that does not readily mix with other fluids (e.g., a flow of bodily fluid that does not mix with a flow or volume of air), a flow with a relatively uniform velocity, and/or the like. 
     While certain aspects and/or features of the embodiment shown in  FIGS. 2-11  are described above, along with ways in which to modify and/or “tune” the aspects and/or features, it should be understood that a flow controller and/or a sequestration chamber (or any structure forming a sequestration chamber) can have any suitable arrangement to result in desired rate, manner, and/or order of conveying the initial volume of bodily fluid into one or more portions or volumes of the sequestration chamber  230 . In some embodiments, a flow controller and/or a sequestration chamber can include and/or can incorporate any suitable combination of the aspects and/or features described above. Any number of the aspects and/or features described above can be included in a device and can act in concert or can act cooperatively to result in the desired fluid flow and/or desired fluid flow characteristics through at least a portion of the sequestration chamber. Moreover, it should be understood that the aspects and/or features described above are provided by way of example only and not limitation. 
     Having transferred the initial volume of bodily fluid into the sequestration chamber  230 , a force can be exerted on the end portion  251  of the actuator  250  to transition and/or place the actuator  250  in its second position, state, operating mode, and/or configuration, as described in above. In some instances, prior to exerting the force on the end portion  251  of the actuator  250 , the actuator  250  may be transitioned from a locked configuration or state to an unlocked configuration or state. In the embodiment shown in  FIGS. 2-11 , the transition of the actuator  250  can be achieved by and/or can otherwise result from user interaction and/or manipulation of the actuator  250 . In other embodiments, however, the transition of the actuator  250  can occur automatically in response to negative pressure and/or associated flow dynamics within the device  200 , and/or enacted by or in response to an external energy source that generates one or more dynamics or states that result in the transitioning of the actuator  250 . 
     As shown in  FIGS. 9-11 , the control device  200  is placed in its third state when each of the flow controller  240  and the actuator  250  is in its second state. When the actuator  250  is transitioned to its second state, position, and/or configuration, the inlet  213  and the outlet  214  are placed in fluid communication (e.g., via a portion of the fluid flow paths  215  and  216  and/or the flow channel  252 ) while the first port  217  and the second port  218  are sequestered, isolated, and/or otherwise not in fluid communication with the inlet  213  and/or the outlet  214 . As such, the initial volume of bodily fluid is sequestered in the portion of the sequestration chamber  230  (e.g., the third volume of the sequestration chamber  230 , as described above). Moreover, in some instances, contaminants such as, for example, dermally residing microbes and/or any other contaminants can be entrained and/or included in the initial volume of the bodily fluid and thus, are sequestered in the sequestration chamber  230  when the initial volume is sequestered therein. As such, the negative pressure previously exerted on or through the fluid flow path  216  and through the second port  218  is now exerted on or through the outlet  214  and the inlet  213  via, for example, at least a portion of the fluid flow paths  215  and  216  and/or the flow channel  252  of the actuator  250  ( FIG. 11 ). In response, bodily fluid can flow from the inlet  213 , through the actuator portion  212  of the housing  210 , through the outlet  214 , and into the fluid collection device coupled to the outlet  214 . Accordingly, the device  200  can function in a manner substantially similar to that of the device  100  described in detail above with reference to  FIG. 1 . 
       FIGS. 12-21  illustrate a fluid control device  300  according to another embodiment. The fluid control device  300  (also referred to herein as “control device” or “device”) can be similar in at least form and/or function to the devices  100  and/or  200  described above. For example, as described above with reference to the devices  100  and  200 , in response to being placed in fluid communication with a negative pressure source (e.g., a suction or vacuum source), the device  300  can be configured to (1) withdraw bodily fluid from a bodily fluid source into the device  300 , (2) divert and sequester a first portion or amount (e.g., an initial volume) of the bodily fluid in a portion of the device  300 , and (3) allow a second portion or amount (e.g., a subsequent volume) of the bodily fluid to flow through the device  300 —bypassing the sequestered initial volume—and into a fluid collection device fluidically coupled to the device  300 . As such, contaminants or the like can be sequestered in or with the initial volume of bodily fluid, leaving the subsequent volume of bodily fluid substantially free of contaminants. In some embodiments, portions and/or aspects of the control device  300  can be similar to and/or substantially the same as portions and/or aspects of the control device  200  described above with reference to  FIGS. 2-11 . Accordingly, such similar portions and/or aspects may not be described in further detail herein. 
     The fluid control device  300  (also referred to herein as “control device” or “device”) includes a housing  310 , a flow controller  340 , and an actuator  350 . In some embodiments, the control device  300  or at least a portion of the control device  300  can be arranged in a modular configuration in which one or more portions of the housing  310  and/or the actuator  350  can be physically and fluidically coupled (e.g., by an end user) to collectively form the control device  300 . Similarly, in some embodiments, the control device  300  can be packaged, shipped, and/or stored independent of a fluid collection device (e.g., a sample reservoir, syringe, etc.) and/or an inlet device (e.g., a needle, catheter, PIV, PICC, etc.), which a user can couple to the control device  300  before or during use. In other embodiments, the control device  300  need not be modular. For example, in some embodiments, the control device  300  can be assembled during manufacturing and delivered to a supplier and/or end user as an assembled device. In some embodiments, the control device  300  can include and/or can be pre-coupled (e.g., during manufacturing and/or prior to being delivered to an end user) to a fluid collection device such as any of those described above. Similarly, in some embodiments, the control device  300  can include and/or can be pre-coupled to an inlet device such as any of those described herein. 
     The housing  310  of the control device  300  can be any suitable shape, size, and/or configuration. The housing  310  includes an actuator portion  312  and a sequestration portion  320 . The actuator portion  312  receives at least a portion of the actuator  350 . The sequestration portion  320  is coupled to a cover  335  and includes, receives, houses, and/or at least partially defines a sequestration chamber  330 . As described in further detail herein, the housing  310  can include and/or can define a first port  317  and a second port  318 , each of which establishes fluid communication between the actuator portion  312  and the sequestration portion  320  of the housing  310  to selectively control and/or allow a flow of fluid through one or more portions of the housing  310 . 
     As shown in  FIGS. 12-16 , the actuator portion  312  of the housing  310  includes an inlet  313  and an outlet  314 , and defines a fluid flow path  315  (e.g., a first fluid flow path) that is configured to selectively place the inlet  313  in fluid communication with the first port  317  and a fluid flow path  316  (e.g., a second fluid flow path) that is configured to selectively place the outlet  314  in fluid communication with the second port  318 . The inlet  313  of the housing  310  is configured to be placed in fluid communication with a bodily fluid source (e.g., in fluid communication with a patient via a needle, IV catheter, PICC line, etc.) to receive a flow of bodily fluid therefrom, as described in detail above. The outlet  314  is configured to be fluidically coupled to a fluid collection device such as any of those described above (e.g., a sample reservoir, a syringe, culture bottle, an intermediary bodily fluid transfer device or adapter, and/or the like). The fluid collection device can define and/or can be manipulated to define a vacuum or negative pressure that results in a negative pressure differential between desired portions of the housing  310  when the fluid collection device is coupled to the outlet  314 . In addition, after an initial volume of bodily fluid has been transferred into the sequestration chamber  330 , fluid communication can be established between the fluid flow paths  315  and  316  to allow a subsequent volume of bodily fluid (e.g., a bodily fluid sample) to flow through the device  300  and into the fluid collection device. Accordingly, the actuator portion  312  of the housing  310  can be substantially similar in at least form and/or function to the actuator portion  212  of the housing  210  and thus, is not described in further detail herein. 
     The sequestration portion  320  of the housing  310  can be any suitable shape, size, and/or configuration. As shown, for example, in  FIGS. 16-18 , the sequestration portion  320  includes and/or forms an inner surface, a portion of which is arranged and/or configured to form a first contoured surface  321 . At least a portion of the first contoured surface  321  can form and/or define a portion of the sequestration chamber  330 , as described in further detail herein. Furthermore, the first port  317  and the second port  318  are configured to form and/or extend through a portion of the first contoured surface  321  to selectively place the sequestration chamber  330  in fluid communication with the fluid flow paths  315  and  316 , as described in further detail here. 
     The sequestration portion  320  is configured to include, form, and/or house, a contour member  325  and the flow controller  340 . More particularly, as shown in  FIGS. 16-18 , the sequestration portion  320  receives and/or is coupled to the contour member  325  such that the flow controller  340  is disposed therebetween. In some embodiments, the contour member  325  can be fixedly coupled to the sequestration portion  320  via an adhesive, ultrasonic welding, and/or any other suitable coupling method. In some embodiments, the contour member  325 , the sequestration portion  320 , and the flow controller  340  can collectively form a substantially fluid tight and/or hermetic seal that isolates the sequestration portion  320  for a volume outside of the sequestration portion  320 . 
     As shown, a cover  335  is configured to be disposed about the contour member  325  such that the cover  335  and the sequestration portion  320  of the housing  310  enclose and/or house the contour member  325  and the flow controller  340 . In some embodiments, the cover  335  can be coupled to the contour member  325  and/or the sequestration portion  320  via an adhesive, ultrasonic welding, one or more mechanical fasteners, a friction fit, a snap fit, a threaded coupling, and/or any other suitable manner of coupling. In some embodiments, the cover  335  can define an opening, window, slot, etc. configured to allow visualization of at least a portion of the sequestration chamber  330 . While the contour member  325  and the cover  335  are described above as being separate pieces and/or components, in other embodiments, the contour member  325  can be integrated and/or monolithically formed with the cover  335 . 
     The contour member  325  includes and/or forms a second contoured surface  326 . The arrangement of the contour member  325  and the sequestration portion  320  of the housing  310  can be such that at least a portion of the first contoured surface  321  is aligned with and/or opposite a corresponding portion of the second contoured surface  326  of the contour member  325  (see e.g.,  FIG. 18 ). As such, a space, volume, opening, void, chamber, and/or the like defined between the first contoured surface  321  and the second contoured surface  326  forms and/or defines the sequestration chamber  330 . Moreover, the flow controller  340  is disposed between the first contoured surface  321  and the second contoured surface  326  and can be configured to transition between a first state and a second state in response to a negative pressure differential and/or suction force applied to at least a portion of the sequestration chamber  330 , as described in further detail herein. 
     The ports  317  and  318  of the housing  310  can be any suitable shape, size, and/or configuration. As described in detail above with reference to the first port  217 , the first port  317  is in fluid communication with a first portion of the sequestration chamber  330  defined between the second contoured surface  326  and a first side of the flow controller  340  and is configured to provide and/or transfer a flow of bodily fluid from the inlet  313  and/or the fluid flow path  315  to the first portion of the sequestration chamber  330  in response to the flow controller  340  transitioning from a first state to a second state. As described above with reference to the second port  218 , the second port  318  is in fluid communication with a second portion of the sequestration chamber  330  defined between the first contoured surface  321  and a second side of the flow controller  340  (e.g., opposite the first side). As such, the second port  318  can be configured to expose the second portion of the sequestration chamber  330  to a negative pressure differential and/or suction force resulting from the fluid collection device operable to transition the flow controller  340  from its first state to its second state, as described in detail above with reference to the device  200 . In addition, the second port  318  can include and/or can be coupled to a restrictor  319  configured to limit and/or restrict a flow of fluid (e.g., air or gas) between the second portion of the sequestration chamber  330  and the fluid flow path  316 , thereby modulating and/or controlling a magnitude of a pressure differential and/or suction force applied on or experienced by the flow controller  340 , as described in detail above with reference to the restrictor  219  of the device  200 . 
     The flow controller  340  disposed in the sequestration portion  320  of the housing  310  can be any suitable shape, size, and/or configuration. Similarly, the flow controller  340  can be formed of any suitable material (e.g., any suitable biocompatible material such as those described herein and/or any other suitable material). For example, the flow controller  340  can be a fluid impermeable bladder configured to be transitioned from a first state and/or configuration to a second state and/or configuration. In some embodiments, the flow controller  340  (e.g., bladder) can include any number of relatively thin and flexible portions configured to deform in response to a pressure differential across the flow controller  340 . In some embodiments, the flow controller  340  can be substantially similar in at least form and/or function to the flow controller  240  described in detail above with reference to  FIGS. 2-11 . For example, in some embodiments, the flow controller  340  can be formed of or from any suitable material and/or can have any suitable durometer such as described the materials and/or durometers described above with reference to the flow controller  240 . Similarly, the flow controller  340  can have a size, shape, surface finish, and/or material property(ies) configured to facilitate, encourage, and/or otherwise result in fluid flow with a desired set of flow characteristics, as described above with reference to the flow controller  240 . Accordingly, portions of the flow controller  340  may not be described in further detail herein. 
     In the embodiment shown in  FIG. 12-21 , the flow controller  340  is a bladder formed of or from silicone having a durometer of about 30 Shore A. The flow controller  340  (e.g., bladder) includes a first deformable portion  341  and a second deformable portion  342 . In addition, the flow controller  340  defines an opening  344  configured to receive at least a portion of the first port  317 , as described above with reference to the flow controller  240 . In some embodiments, the flow controller  340  can include one or more portions configured to form one or more seals with and/or between the flow controller  340  and each of the contoured surfaces  321  and  326 , as described in further detail herein. 
     The deformable portions  341  and  342  of the flow controller  340  can be relatively thin and flexible portions configured to deform in response to a pressure differential between the first side of the flow controller  340  and the second side of the flow controller  340 . More particularly, the deformable portions  341  and  342  can each have a thickness of about 0.005″. As shown, for example, in  FIGS. 18 and 20 , the deformable portions  341  and  342  of the flow controller  340  correspond to and/or have substantially the same general shape as at least a portion of the contoured surfaces  321  and/or  326 . As such, the deformable portions  341  and  342  and the corresponding portion(s) of the contoured surfaces  321  and/or  326  can collectively form and/or define one or more channels, volumes, and/or the like, which in turn, can receive the initial volume of bodily fluid, as described in further detail herein. 
     As described above with reference to the flow controller  240 , the flow controller  340  is configured to transition between a first state and a second state. For example, when the flow controller  340  is in its first state, the first deformable portion  341  can be disposed adjacent to and/or substantially in contact with a first recess  327  formed by the second contoured surface  326  and the second deformable portion  342  can be disposed adjacent to and/or substantially in contact with a second recess  328  formed by the second contoured surface  326 . As such, the first portion of the sequestration chamber  330  (e.g., the portion defined between the second contoured surface  326  and the first surface of the flow controller  340 ) can have a relatively small and/or relatively negligible volume. In contrast, when the flow controller  340  is transitioned from its first state to its second state (e.g., in response to a negative pressure applied and/or transmitted via the second port  318 ), the first deformable portion  341  can be disposed adjacent to and/or substantially in contact with a first recess  322  formed by the first contoured surface  321  and the second deformable portion  342  can be disposed adjacent to and/or substantially in contact with a second recess  323  formed by the first contoured surface  321 . Accordingly, a volume of the first portion of the sequestration chamber  330  is larger when the flow controller  340  is in its second state than when the flow controller is in its first state. As described in detail above with reference to the sequestration chamber  230  and flow controller  240 , the increase in the volume of the first portion of the sequestration chamber  330  can result in a negative pressure or vacuum therein that can be operable to draw a volume of air or gas as well as the initial volume of bodily fluid into the sequestration chamber  330 . 
     While the flow controller  340  is particularly shown and described, in other embodiments, the flow controller  340  and/or the sequestration chamber  330  can have any suitable configuration and/or arrangement. For example, in some embodiments, the contoured surfaces  321  and/or  326  can include more or fewer recesses (e.g., the recesses  322  and  323  and the recesses  327  and  328 ). In other embodiments, a depth of one or more recesses can be modified. Similarly, the flow controller  340  can be modified in any suitable manner to substantially correspond to a shape and/or configuration of the contoured surfaces  321  and/or  326 . While the flow controller  340  is described as being a bladder or the like including a number of deformable portions, in other embodiments, a flow controller can be arranged and/or configured as, for example, a bellows, a flexible pouch, an expandable bag, an expandable chamber, a plunger (e.g., similar to a syringe), and/or any other suitable reconfigurable container or the like. In addition, the sequestration chamber  330  at least partially formed by the flow controller  340  can have any suitable shape, size, and/or configuration. 
     The actuator  350  of the control device  300  can be any suitable shape, size, and/or configuration. At least a portion of the actuator  350  is disposed within the actuator portion  312  of the housing  310  and is configured to be transitioned between a first state, configuration, and/or position and a second state, configuration, and/or position. In the embodiment shown in  FIGS. 12-21 , the actuator  350  is configured as an actuator rod or plunger configured to be moved relative to the actuator portion  312  of the housing  310 . The actuator  350  includes a set of seals  355  and defines a flow channel  352 . The actuator  350  further includes an end portion  351  disposed outside of the housing  310  and configured to be engaged by a user to transition the actuator  350  between its first state, in which the fluid flow path  315  can establish fluid communication between the inlet  313  and the first port  317 , and its second state, in which (1) the first port  317  (and thus, the sequestration chamber  330 ) are sequestered and/or fluidically isolated and (2) the inlet  313  and the outlet  314  are placed in fluid communication via at least a portion of the fluid flow paths  315  and  316  and/or the flow channel  352  of the actuator  350 . As such, the actuator  350  is similar in form and/or function to the actuator  250  described above with reference to  FIGS. 2-11 . Thus, the actuator  350  is not described in further detail herein. 
     The device  300  can be used to procure a bodily fluid sample having reduced contamination (e.g., contamination from microbes such as, for example, dermally residing microbes, microbes external to the bodily fluid source, and/or the like) in a manner substantially similar to the manner described above with reference to the device  200 . For example, prior to use, the device  300  can be in its first, initial, and/or storage state or operating mode, in which each of the flow controller  340  and the actuator  350  is in its respective first or initial state. With the device  300  in the first state, a user such as a doctor, physician, nurse, phlebotomist, technician, etc. can manipulate the device  300  to establish fluid communication between the inlet  313  and the bodily fluid source (e.g., a vein of a patient). Once the inlet  313  is placed in fluid communication with the bodily fluid source, the outlet  314  can be fluidically coupled to a fluid collection device (not shown in  FIGS. 12-21 ). In the embodiment shown in  FIGS. 12-21 , for example, the fluid collection device can be an evacuated container, a culture bottle, a sample reservoir, a syringe, and/or any other suitable container or device configured to define or produce a negative pressure, suction force, vacuum, and/or energy potential. 
     When the actuator  350  is in the first position and/or configuration, the inlet  313  of the housing  310  is in fluid communication with, for example, the fluid flow path  315 , which in turn, is in fluid communication with the first port  317  (see e.g.,  FIGS. 17 and 18 ). The outlet  314  of the of the housing  310  is in fluid communication with the fluid flow path  316 , which in turn, is in fluid communication with the second port  318  (see e.g.,  FIGS. 17 and 18 ). As described in detail above, when the control device  300  is in the first state or operating mode (e.g., when the actuator  350  and the flow controller  340  are each in their first state), fluidically coupling the fluid collection device to the outlet  314  generates and/or otherwise results in a negative pressure differential and/or suction force within at least a portion of the fluid flow path  316  and, in turn, within the portion of the sequestration chamber  330  defined between a surface of the flow controller  340  (e.g., a first surface) and the first contoured surface  321  of the housing  310 . 
     The flow controller  340  is in the first state and/or configuration prior to the fluid collection device being coupled to the outlet  314 . In the embodiment shown in  FIGS. 12-21 , the flow controller  340  is a fluid impermeable bladder and/or the like that can have a flipped, inverted, collapsed, and/or empty configuration (e.g., the first state and/or configuration) prior to coupling the fluid collection device to the outlet  314 . For example, as shown in  FIG. 18 , the flow controller  340  can be disposed adjacent to and/or in contact with the second contoured surface  326  when the flow controller  340  is in its first state and/or configuration. 
     As described above, the flow controller  340  is configured to transition from its first state and/or configuration to its second state and/or configuration in response to the negative pressure differential and/or suction force generated within the portion of the sequestration chamber  330  defined between the flow controller  340  and the first contoured surface  321 . For example, the flow controller  340  can be disposed adjacent to and/or in contact with the second contoured surface  326  when the flow controller  340  is in its first state ( FIG. 18 ) and can be transitioned, moved, “flipped”, placed, and/or otherwise reconfigured into its second state in which the flow controller  340  is disposed adjacent to and/or in contact with the first contoured surface  321  ( FIG. 20 ). Moreover, the control device  300  is placed in its second state and/or configuration when the actuator  350  is in its first state and the flow controller  340  is in its second state. 
     The transitioning of the flow controller  340  results in an increase in an inner volume of the portion of the sequestration chamber  330  defined between a surface of the flow controller  340  (e.g., a second surface opposite the first surface) and the second contoured surface  326 . As described in detail above with reference to the device  200 , the increase in the inner volume can, in turn, result in a negative pressure differential between the portion of the sequestration chamber  330  (defined at least in part by the flow controller  340 ) and, for example, the inlet  313  that is operable in drawing at least a portion of an initial flow, amount, or volume of bodily fluid from the inlet  313 , through the fluid flow path  315  and the first port  317 , and into the portion of the sequestration chamber  330 . In some instances, the initial volume and/or flow of bodily fluid can be transferred into the sequestration chamber  330  until, for example, the flow controller  340  is fully expanded, flipped, and/or transitioned, until the negative pressure differential is reduced and/or equalized, and/or until a desired volume of bodily fluid is disposed within the portion of the sequestration chamber  330 . Moreover, the restrictor  319  can be configured to restrict, limit, control, and/or modulate a magnitude of the negative pressure differential and/or suction force generated within the sequestration chamber  330  and/or on a surface of the flow controller  340 , which in turn, can modulate a suction force within one or more flow paths and/or within the bodily fluid source (e.g., the vein of the patient), as described above with reference to the device  200 . In other embodiments, the second port  318  and/or any suitable portion of the device  300  can be configured to modulate a suction force within one or more portions of the sequestration chamber  330  in any suitable manner such as, for example, those described above with reference to the device  200 . 
     In some embodiments, the shape, size, and/or arrangement of the sequestration chamber  330  and/or the flow controller  340 , the magnitude of the negative pressure differential or suction force, and/or the way in which the negative pressure differential or suction force is exerted can dictate and/or control a rate and/or manner in which the flow controller  340  is transitioned from the first state to the second state. For example, while the flow controller  240  is described above as including the first deformable portion  241 , the second deformable portion  242 , and the third deformable portion  243 , the flow controller  340  included in the embodiment shown in  FIGS. 12-21  includes only the first deformable portion  341  and the second deformable portion  342 . Moreover, as shown in  FIGS. 18 and 20 , the recesses  322  and  323  of the first contoured surface  321  have substantially the same depth. In some embodiments, such an arrangement can, for example, limit and/or reduce an amount of negative pressure and/or suction force sufficient to transition and/or flip the first deformable portion  341  relative to the amount of negative pressure and/or suction force sufficient to transition and/or flip the first and second deformable portions  341  and  342  of the flow controller  340 . 
     As described above, in some embodiments, the first deformable portion  341  can have a thickness and/or stiffness that is greater than a thickness and/or stiffness of the second deformable portion  342  such that the second deformable portion  342  completes or substantially completes its transition and/or flip before the first deformable portion  341  completes or substantially completes its transition and/or flip. In other embodiments, the flow controller  340  can include any suitable feature, structure, material property, surface finish, and/or the like, and/or any other portion of the device  300  can include any suitable feature, structure, etc. configured to control an order and/or manner in which the flow controller  340  transitions from the first state to the second state, such as any of those described above with reference to the flow controller  240 . In some embodiments, the arrangement of the flow controller  340  may result in the device  300  being compatible with fluid collection devices having a relatively low amount of negative pressure. In some embodiments, such an arrangement may also facilitate and/or simplify one or more manufacturing processes and/or the like. In some instances, controlling the rate, order, and/or manner can result in one or more desired flow characteristic associated with a flow of air, gas, and/or bodily fluid into and/or through at least a portion of the sequestration chamber  230 . 
     As described above with reference to the deformable portions  241  and  242 , the first deformable portion  341  and the first recess  327  of the second contoured surface  326  (e.g., a first volume of the sequestration chamber  330 ) can be configured to receive a volume of air that was within the fluid flow path between the bodily fluid source and the sequestration chamber  330  prior to the fluid flow path receiving and/or being filled with the flow of bodily fluid. In other words, the transitioning of the flow controller  340  can vent or purge air or gas from the fluid flow path between the bodily fluid source and the sequestration chamber  330 , which can then be stored or contained within the first and second volumes of the sequestration chamber  330 . On the other hand, a portion of the sequestration chamber  330  collectively defined by the second deformable portion  342  and the second recess  328  of the second contoured surface  326  (e.g., a second volume of the sequestration chamber  330 ) can be configured to receive the initial volume of bodily fluid that flows through the fluid flow path between the bodily fluid source and the sequestration chamber  330  after the air or gas is vented and/or purged. Thus, as described above with reference to the device  200 , the initial volume can be transferred into the sequestration chamber  330 . 
     In some instances, the arrangement of the sequestration chamber  330  and/or the flow controller  340  can result in an even flow of the initial volume of bodily fluid into, for example, the second volume of the sequestration chamber  330 . For example, as described in detail above with reference to the device  200 , the sequestration chamber  330  and/or the flow controller  340  can be configured and/or arranged such that bodily fluid flows into and/or through at least a portion of the sequestration chamber  330  (e.g., the second volume of the sequestration chamber  330 ) with a uniform flow front and substantially without mixing with a volume of air in the sequestration chamber  330 . In other embodiments, a flow controller can have any other suitable arrangement to result in desired rate, manner, and/or order of conveying the initial volume of bodily fluid into one or more portions or volumes of the sequestration chamber  330  such as, for example, any of those described above with reference to the device  200 . 
     Having transferred the initial volume of bodily fluid into the sequestration chamber  330 , a force can be exerted on the end portion  351  of the actuator  350  to transition and/or place the actuator  350  in its second position, state, operating mode, and/or configuration, as described in above. In some instances, prior to exerting the force on the end portion  351  of the actuator  350 , the actuator  350  may be transitioned from a locked configuration or state to an unlocked configuration or state. In the embodiment shown in  FIGS. 12-21 , the transition of the actuator  350  can be achieved by and/or can otherwise result from user interaction and/or manipulation of the actuator  350 . In other embodiments, however, the transition of the actuator  350  can occur automatically in response to negative pressure and/or associated flow dynamics within the device  300 , and/or enacted by or in response to an external energy source that generates one or more dynamics or states that result in the transitioning of the actuator  350 . 
     As shown in  FIGS. 19-21 , the control device  300  is placed in its third state when each of the flow controller  340  and the actuator  350  is in its second state. When the actuator  350  is transitioned to its second state, position, and/or configuration, the inlet  313  and the outlet  314  are placed in fluid communication (e.g., via the fluid flow path  316  and/or the flow channel  352 ) while the fluid flow path  315  and/or the first port  317  is/are sequestered, isolated, and/or otherwise not in fluid communication with the inlet  313  and/or the outlet  314 . As such, the initial volume of bodily fluid is sequestered in the portion of the sequestration chamber  330  (e.g., the third volume of the sequestration chamber  330 , as described above). Moreover, in some instances, contaminants such as, for example, dermally residing microbes and/or any other contaminants can be entrained and/or included in the initial volume of the bodily fluid and thus, are sequestered in the sequestration chamber  330  when the initial volume is sequestered therein. As such, the negative pressure otherwise exerted on or through the fluid flow path  316  and through the second port  318  is now exerted on or through the outlet  314  and the inlet  313  via, for example, at least a portion of the fluid flow paths  315  and  316  and/or the flow channel  352  of the actuator  350  ( FIG. 21 ). In response, bodily fluid can flow from the inlet  313 , through the actuator portion  312  of the housing  310 , through the outlet  314 , and into the fluid collection device coupled to the outlet  314 . Accordingly, the device  300  can function in a manner substantially similar to that of the devices  100  and/or  200  described in detail above. 
       FIGS. 22-27  illustrate a fluid control device  400  according to another embodiment. The fluid control device  400  (also referred to herein as “control device” or “device”) can be similar in at least form and/or function to the devices  100 ,  200 , and/or  300  described above. For example, as described above with reference to the devices  100 ,  200 , and/or  300 , in response to being placed in fluid communication with a negative pressure source (e.g., a suction or vacuum source), the device  400  can be configured to (1) withdraw bodily fluid from a bodily fluid source into the device  400 , (2) divert and sequester a first portion or amount (e.g., an initial volume) of the bodily fluid in a portion of the device  400 , and (3) allow a second portion or amount (e.g., a subsequent volume) of the bodily fluid to flow through the device  400 —bypassing the sequestered initial volume—and into a fluid collection device fluidically coupled to the device  400 . As such, contaminants or the like can be sequestered in or with the initial volume of bodily fluid, leaving the subsequent volume of bodily fluid substantially free of contaminants. In some embodiments, portions and/or aspects of the control device  400  can be similar to and/or substantially the same as portions and/or aspects of at least the control device  200  described above with reference to  FIGS. 2-11 . Accordingly, such similar portions and/or aspects may not be described in further detail herein. 
     The fluid control device  400  includes a housing  410 , a flow controller  440 , and an actuator  450 . In some embodiments, the control device  400  or at least a portion of the control device  400  can be arranged in a modular configuration (e.g., including one or more independent or separate components that are later assembled) or can be arranged in an integrated or at least partially integrated configuration (e.g., including one or more components that are pre-assembled or pre-coupled), as described above with reference to the device  200 . For example, in some embodiments, the control device  400  can include and/or can be coupled to a fluid collection device and/or an inlet device such as any of those described above. 
     The housing  410  of the control device  400  can be any suitable shape, size, and/or configuration. In general, the housing  410  can be substantially similar in at least form and/or function to the housing  210 . Accordingly, while certain components, features, aspects, and/or functions of the housing  410  are identified in the drawings and discussed below, such similarities are not described in further detail herein and should be considered similar to the corresponding components, features, aspects, and/or functions described above with reference to the device  200  unless explicitly described to the contrary. 
     The housing  410  includes an actuator portion  412  and a sequestration portion  420 . The actuator portion  412  receives at least a portion of the actuator  450 . The sequestration portion  420  is coupled to a cover  435  and includes, receives, houses, and/or at least partially defines a sequestration chamber  430 . As described in further detail herein, the housing  410  can include and/or can define a first port  417  and a second port  418 , each of which establishes fluid communication between the actuator portion  412  and the sequestration portion  420  of the housing  410  to selectively control and/or allow a flow of fluid through one or more portions of the housing  410 . 
     The actuator portion  412  of the housing  410  includes an inlet  413  and an outlet  414 , and defines a fluid flow path  415  (e.g., a first fluid flow path) that is configured to selectively place the inlet  413  in fluid communication with the first port  417  and a fluid flow path  416  (e.g., a second fluid flow path) that is configured to selectively place the outlet  414  in fluid communication with the second port  418 . The actuator portion  412  of the housing  410  can be substantially similar in at least form and/or function to the actuator portion  212  of the housing  210  and thus, is not described in further detail herein. 
     The sequestration portion  420  of the housing  410  can be any suitable shape, size, and/or configuration. The sequestration portion  420  is configured to include, form, and/or house, a contour member  425  and the flow controller  440 . More specifically, a cover  435  is configured to be disposed about the contour member  425  such that the cover  435  and the sequestration portion  420  of the housing  410  enclose and/or house the contour member  425  and the flow controller  440 . The sequestration portion  420  of the housing  410  and/or components thereof or coupled thereto can be substantially similar in at least form and/or function to the sequestration portion  220  of the housing  210  (and/or components thereof or coupled thereto) and thus, is/are not described in further detail herein. 
     As shown for example, in  FIGS. 24-27 , the sequestration portion  420  includes and/or forms an inner surface, a portion of which is arranged and/or configured to form a first contoured surface  421 . At least a portion of the first contoured surface  421  can form and/or define a portion of the sequestration chamber  430 , as described in further detail herein. Furthermore, the first port  417  and the second port  418  are configured to form and/or extend through a portion of the first contoured surface  421  to selectively place the sequestration chamber  430  in fluid communication with the fluid flow paths  415  and  416 , as described above with reference to the device  200 . 
     The first contoured surface  421  can be any suitable shape, curvature, and/or texture, and can, for example, be substantially similar to the first contoured surface  221  of the housing  220 . For example, the first contoured surface  421  includes and/or forms at least a first recess  422  and a second recess  423 . The first contour surface  421  can differ from the first contoured surface  221 , however, by including any number of ventilation ridges  424 , as shown in  FIGS. 24-27 . The distribution of the ventilation ridges  424  on the first contour surface  421  can include multiple arrangements. For example, the first contour surface  421  can have one ventilation ridge  424 , multiple ventilation ridges  424 , multiple concentric ventilation ridges  424 , etc. disposed within and/or formed by the first recess  422  and/or one ventilation ridge  424 , multiple ventilation ridges  424 , or multiple concentric ventilation ridges  424  disposed within and/or formed by the second recess  423  of the first contour surface  421 , as shown in  FIGS. 25 and 27 . In some implementations, the ventilation ridges  424  are configured to reduce and/or control the ability or the likelihood of the flow controller  440  or portions thereof forming a seal when placed in contact with the first contour surface  421  in response to a negative pressure applied and/or transmitted via the second port  418  (e.g., a negative pressure in a volume between the first contoured surface  421  and the flow controller  440 ). Said another way, the ventilation ridges  424  can form discontinuities along one or more portions of the first contoured surface  421  that, for example, can prevent air from being trapped in localized areas between the flow controller  440  and one or more portions of the first contour surface  421  by allowing air to flow freely between the flow controller  440  and one or more portions of the first contour surface  421 , as described in further detail herein. 
     As shown in  FIGS. 24-27 , the sequestration portion  420  receives and/or is coupled to the contour member  425  such that the flow controller  440  is disposed therebetween. In some embodiments, the contour member  425  can be substantially similar in at least form and/or function to the contour member  225  described above with reference to the device  200 . For example, the contoured member  425  includes and/or forms a second contoured surface  426 . The second contour surface  426  can be any suitable shape, curvature, and/or texture, and can, for example, be substantially similar to the second contoured surface  226  of the housing  220 . For example, the second contoured surface  426  includes and/or forms a first recess  427 , a second recess  428 , and a third recess  429 . The second contour surface  426  can differ from the second contoured surface  226 , however, by including any number of ventilation channels  431 , as shown in  FIGS. 24-27 . The distribution of the ventilation channels  431  on the second contour surface  426  can include multiple arrangements. For example, the second contour surface  426  can be configured to have one ventilation channel  431 , multiple ventilation channels  431 , or multiple concentric ventilation channels  431  disposed within and/or formed by the first recess  427  and/or one ventilation channel  431 , multiple ventilation channels  431 , or multiple concentric ventilation channels  431  disposed within and/or formed by the second recess  428  of the second contour surface  426 , as shown in  FIGS. 25 and 27 . The ventilation channels  431  are configured to reduce and/or control the ability or the likelihood of the flow controller  440  or portions thereof forming a seal when placed in contact with the second contour surface  426  in response to a positive pressure (e.g., in a volume between the first contoured surface  421  and the flow controller  440 ), as described above with reference to the ventilation ridges  424 . 
     While the first contour surface  421  is described above as including the ventilation ridges  424  and the second contour surface  426  is described above as including the ventilation channels  431 , it should be understood that the ventilation ridges  424  and the ventilation channels  431  have been presented by way of example only and not limitation. Various alternatives and/or combinations are contemplated. For example, in some embodiments, the first contour surface  421  can include ventilation channels while the second contour surface  426  can include ventilation ridges. In other embodiments, the first contour surface  421  and/or the second contour surface  426  can include a combination of ventilation channels and ventilation ridges. As such, the contour surfaces  421  and  426  can include one or more discontinuity having any suitable shape, size, and/or configuration that can allow for and/or otherwise ensure that air can flow between the flow controller  440  and the contour surfaces  421  and  426 . Moreover, while each of the contour surfaces  421  and  426  is shown as including a ventilation feature or discontinuity, in other embodiments, the first contour surface  421  can include a ventilation feature or discontinuity while the second contour surface  426  does not, or vice versa. 
     The flow controller  440  disposed in the sequestration portion  420  of the housing  410  can be any suitable shape, size, and/or configuration. Similarly, the flow controller  440  can be formed of any suitable material (e.g., any suitable biocompatible material such as those described herein and/or any other suitable material). For example, the flow controller  440  can be a fluid impermeable bladder configured to be transitioned from a first state and/or configuration to a second state and/or configuration. In some embodiments, the flow controller  440  (e.g., bladder) can include any number of relatively thin and flexible portions configured to deform in response to a pressure differential across the flow controller  440 . In some embodiments, the flow controller  440  can be substantially similar in at least form and/or function to the flow controller  240  described in detail above with reference to  FIGS. 2-11 . For example, in some embodiments, the flow controller  440  can be formed of or from any suitable material and/or can have any suitable durometer such as described the materials and/or durometers described above with reference to the flow controller  240 . Similarly, the flow controller  440  can have a size, shape, surface finish, and/or material property(ies) configured to facilitate, encourage, and/or otherwise result in fluid flow with a desired set of flow characteristics, as described above with reference to the flow controller  240 . Accordingly, portions of the flow controller  440  may not be described in further detail herein. 
     In the embodiment shown in  FIG. 22-27 , the flow controller  440  is a bladder formed of or from silicone having a durometer of about 30 Shore A. The flow controller  440  (e.g., bladder) includes a first deformable portion  441 , a second deformable portion  442 , and a third deformable portion  443 . In addition, the flow controller  440  defines an opening  444  configured to receive at least a portion of the first port  417 , as described above with reference to the flow controller  240 . In some embodiments, the flow controller  440  can include one or more portions configured to form one or more seals with and/or between the flow controller  440  and each of the contoured surfaces  421  and  426 . For example, as shown in  FIGS. 24-27 , the deformable portions  441 ,  442  and  443  of the flow controller  440  correspond to and/or have substantially the same general shape as at least a portion of the contoured surfaces  421  and/or  426 . As such, the deformable portions  441 ,  442  and  443  and the corresponding portion(s) of the contoured surfaces  421  and/or  426  can collectively form and/or define one or more volumes, and/or the like, which in turn, can receive the initial volume of bodily fluid, as described in further detail herein. 
     As described above with reference to the flow controller  240 , the flow controller  440  is configured to transition between a first state and a second state. For example, when the flow controller  440  is in its first state, the first deformable portion  441  can be disposed adjacent to and/or substantially in contact with a first recess  427  formed by the second contoured surface  426 , the second deformable portion  442  can be disposed adjacent to and/or substantially in contact with a second recess  428 , and the third deformable portion  443  can be disposed adjacent to and/or substantially in contact with a second recess  429  formed by the second contoured surface  426 . As such, the first portion of the sequestration chamber  430  (e.g., the portion defined between the second contoured surface  426  and the first surface of the flow controller  440 ) can have a relatively small and/or relatively negligible volume. In contrast, when the flow controller  440  is transitioned from its first state to its second state (e.g., in response to a negative pressure applied and/or transmitted via the second port  418 ), at least the deformable portions  441 ,  442 , and  443  are disposed adjacent to and/or substantially in contact with the first contoured surface  421 . More specifically, the first deformable portion  421  can be disposed adjacent to and/or substantially in contact with a first recess  422  formed by the first contoured surface  421 , the second deformable portion  442  can be disposed adjacent to and/or substantially in contact with a second recess  423  formed by the first contoured surface  421 , and the third deformable portion  243  can be disposed adjacent to and/or substantially in contact with, for example, a non-recessed portion of the first contoured surface  421 , as described above with reference to the flow controller  240 . 
     The actuator  450  of the control device  400  can be any suitable shape, size, and/or configuration. At least a portion of the actuator  450  is disposed within the actuator portion  412  of the housing  410  and is configured to be transitioned between a first state, configuration, and/or position and a second state, configuration, and/or position. In the embodiment shown in  FIGS. 22-27 , the actuator  450  is configured as an actuator rod or plunger configured to be moved relative to the actuator portion  412  of the housing  410 . The actuator  450  includes a set of seals  455  and defines a flow channel  452 . The actuator  450  further includes an end portion  451  disposed outside of the housing  410  and configured to be engaged by a user to transition the actuator  450  between its first state, in which the fluid flow path  415  can establish fluid communication between the inlet  413  and the first port  417 , and its second state, in which (1) the first port  417  (and thus, the sequestration chamber  430 ) are sequestered and/or fluidically isolated and (2) the inlet  413  and the outlet  414  are placed in fluid communication via at least a portion of the fluid flow paths  415  and  416  and/or the flow channel  452  of the actuator  450 . As such, the actuator  450  is similar in form and/or function to the actuator  250  described above with reference to  FIGS. 2-11 . Thus, the actuator  450  is not described in further detail herein. 
     The device  400  can be used to procure a bodily fluid sample having reduced contamination (e.g., contamination from microbes such as, for example, dermally residing microbes, microbes external to the bodily fluid source, and/or the like) in a manner substantially similar to the manner described above with reference to the device  200 . For example, prior to use, the device  400  can be in its first, initial, and/or storage state or operating mode, in which each of the flow controller  440  and the actuator  450  is in its respective first or initial state. With the device  400  in the first state, a user such as a doctor, physician, nurse, phlebotomist, technician, etc. can manipulate the device  400  to establish fluid communication between the inlet  413  and the bodily fluid source (e.g., a vein of a patient). Once the inlet  413  is placed in fluid communication with the bodily fluid source, the outlet  414  can be fluidically coupled to a fluid collection device (not shown in  FIGS. 22-27 ). In the embodiment shown in  FIGS. 22-27 , for example, the fluid collection device can be an evacuated container, a culture bottle, a sample reservoir, a syringe, and/or any other suitable container or device configured to define or produce a negative pressure, suction force, vacuum, and/or energy potential. 
     When the actuator  450  is in the first position and/or configuration, the inlet  413  of the housing  410  is in fluid communication with, for example, the fluid flow path  415 , which in turn, is in fluid communication with the first port  417 . The outlet  414  of the of the housing  410  is in fluid communication with the fluid flow path  416 , which in turn, is in fluid communication with the second port  418  (see e.g.,  FIG. 24 ). As described in detail above, when the control device  400  is in the first state or operating mode (e.g., when the actuator  450  and the flow controller  440  are each in their first state), fluidically coupling the fluid collection device to the outlet  414  generates and/or otherwise results in a negative pressure differential and/or suction force within at least a portion of the fluid flow path  416  and, in turn, within the portion of the sequestration chamber  430  defined between a surface of the flow controller  440  (e.g., a first surface) and the first contoured surface  421  of the housing  410 . 
     The flow controller  440  is in the first state and/or configuration prior to the fluid collection device being coupled to the outlet  414 . In the embodiment shown in  FIGS. 22-27 , the flow controller  440  is a fluid impermeable bladder and/or the like that can have a flipped, inverted, collapsed, and/or empty configuration (e.g., the first state and/or configuration) prior to coupling the fluid collection device to the outlet  414 . For example, as shown in  FIGS. 24 and 25 , the flow controller  440  can be disposed adjacent to and/or in contact with the second contoured surface  426  when the flow controller  440  is in its first state and/or configuration. 
     As described above, the controller  440  is configured to transition from its first state and/or configuration to its second state and/or configuration in response to the negative pressure differential and/or suction force generated within the portion of the sequestration chamber  430  defined between the flow controller  440  and the first contoured surface  421 . For example, the flow controller  440  can be disposed adjacent to and/or in contact with the second contoured surface  426  when the flow controller  440  is in its first state ( FIGS. 24 and 25 ) and can be transitioned, moved, “flipped”, placed, and/or otherwise reconfigured into its second state in which the flow controller  440  is disposed adjacent to and/or in contact with the first contoured surface  421  ( FIGS. 26 and 27 ). Moreover, the ventilation channels  431  formed by the second contour surface  426  can allow air to flow between the second contoured surface  426  and the flow controller  440 , which can, in some instances, reduce a likelihood of pockets of air being trapped between the second contoured surface  426  and the flow controller  440  if and/or when a positive pressure is applied in a volume between the flow controller  440  and the first contoured surface  421  via the port  418  (e.g., a positive pressure that drives and/or urges the flow controller  440  toward the second contoured surface  426  such as during manufacturing, testing, and/or use). 
     The control device  400  is placed in its second state and/or configuration when the actuator  450  is in its first state and the flow controller  440  is in its second state. The transitioning of the flow controller  440  results in an increase in an inner volume of the portion of the sequestration chamber  430  defined between a surface of the flow controller  440  (e.g., a second surface opposite the first surface) and the second contoured surface  426 . As described in detail above with reference to the device  200 , the increase in the inner volume can, in turn, result in a negative pressure differential between the portion of the sequestration chamber  430  (defined at least in part by the flow controller  440 ) and, for example, the inlet  413  that is operable in drawing at least a portion of an initial flow, amount, or volume of bodily fluid from the inlet  413 , through the fluid flow path  415  and the first port  417 , and into the portion of the sequestration chamber  430 . In some instances, the initial volume and/or flow of bodily fluid can be transferred into the sequestration chamber  430  until, for example, the flow controller  440  is fully expanded, flipped, and/or transitioned, until the negative pressure differential is reduced and/or equalized, and/or until a desired volume of bodily fluid is disposed within the portion of the sequestration chamber  430 . Moreover, the restrictor  419  can be configured to restrict, limit, control, and/or modulate a magnitude of the negative pressure differential and/or suction force generated within the sequestration chamber  430  and/or on a surface of the flow controller  440 , which in turn, can modulate a suction force within one or more flow paths and/or within the bodily fluid source (e.g., the vein of the patient), as described above with reference to the device  200 . In other embodiments, the second port  418  and/or any suitable portion of the device  400  can be configured to modulate a suction force within one or more portions of the sequestration chamber  30  in any suitable manner such as, for example, those described above with reference to the device  200 . 
     In some embodiments, the shape, size, and/or arrangement of the sequestration chamber  430  and/or the flow controller  440 , the ventilation channels  431  and/or the ventilation ridges  424 , the magnitude of the negative pressure differential or suction force, and/or the way in which the negative pressure differential or suction force is exerted can dictate and/or control a rate and/or manner in which the flow controller  440  is transitioned from the first state to the second state. In some instances, controlling the rate, order and/or manner in which the flow controller  440  is transitioned can result in one or more desired flow characteristics associated with a flow of air, gas, and/or bodily fluid into and/or through at least a portion of the sequestration chamber. For example, the arrangement included in this embodiment can be such that a transitioning and/or flipping of the third deformable portion  443  of the flow controller  440  is completed prior to completion of the transitioning and/or flipping of the first and second deformable portions  441  and  442 . Moreover, the arrangement of the ventilation ridges  424  along the first contoured surface  421  can increase a likelihood and/or can ensure that the flow controller  440  transitions and/or flips in a desired manner or sequence by preventing potential flow restrictions and/or seals that may otherwise prevent the negative pressure differential or suction force from transitioning and/or flipping a portion of the flow controller  440  disposed on an opposite side of the restriction or seal. 
     This arrangement can be such that a portion of the sequestration chamber  430  collectively defined by the first deformable portion  441  and the first recess  427  of the second contoured surface  426  (e.g., a first volume of the sequestration chamber  430 ) receives at least a portion of a volume of air that was within the fluid flow path between the bodily fluid source and the sequestration chamber  430  prior to the fluid flow path receiving and/or being filled with bodily fluid. Similarly, a portion of the sequestration chamber  430  collectively defined by the second deformable portion  442  and the second recess  428  of the second contoured surface  426  (e.g., a second volume of the sequestration chamber  430 ) can receive at least a portion of the volume of air that was within the fluid flow path. Alternative arrangements of the sequestration chamber  430  and/or the flow controller  440  can be similar in form and function to those described above with reference to the sequestration chamber  230  and/or the flow controller  240 , and thus they are not described in further detail herein. 
     Having transferred the initial volume of bodily fluid into the sequestration chamber  430 , a force can be exerted on the end portion  451  of the actuator  450  to transition and/or place the actuator  450  in its second position, state, operating mode, and/or configuration, as described in above. In some instances, prior to exerting the force on the end portion  451  of the actuator  450 , the actuator  450  may be transitioned from a locked configuration or state to an unlocked configuration or state. In the embodiment shown in  FIGS. 22-27 , the transition of the actuator  450  can be achieved by and/or can otherwise result from user interaction and/or manipulation of the actuator  450 . In other embodiments, however, the transition of the actuator  450  can occur automatically in response to negative pressure and/or associated flow dynamics within the device  400 , and/or enacted by or in response to an external energy source that generates one or more dynamics or states that result in the transitioning of the actuator  450 . 
     As shown in  FIGS. 26 and 27 , the control device  400  is placed in its third state when each of the flow controller  440  and the actuator  450  is in its second state. When the actuator  450  is transitioned to its second state, position, and/or configuration, the inlet  413  and the outlet  414  are placed in fluid communication (e.g., via the fluid flow path  416  and/or the flow channel  452 ) while the fluid flow path  415  and/or the first port  417  is/are sequestered, isolated, and/or otherwise not in fluid communication with the inlet  413  and/or the outlet  414 . As such, the initial volume of bodily fluid is sequestered in the portion of the sequestration chamber  430 . Moreover, in some instances, contaminants such as, for example, dermally residing microbes and/or any other contaminants can be entrained and/or included in the initial volume of the bodily fluid and thus, are sequestered in the sequestration chamber  430  when the initial volume is sequestered therein. As such, the negative pressure otherwise exerted on or through the fluid flow path  416  and through the second port  418  is now exerted on or through the outlet  414  and the inlet  413  via, for example, at least a portion of the fluid flow paths  415  and  416  and/or the flow channel  452  of the actuator  450 . In response, bodily fluid can flow from the inlet  413 , through the actuator portion  412  of the housing  410 , through the outlet  414 , and into the fluid collection device coupled to the outlet  414 . Accordingly, the device  400  can function in a manner substantially similar to that of the devices  100  and/or  200  described in detail above. 
     Referring now to  FIG. 28 , a flowchart is presented illustrating a method  10  of using a fluid control device to obtain a bodily fluid sample with reduced contamination according to an embodiment. The fluid control device can be similar to and/or substantially the same as any of the fluid control devices  100 ,  200 ,  300 , and/or  400  described in detail above. Accordingly, the fluid control device (also referred to herein as “control device” or “device”) can include a housing, a flow controller, and an actuator. The method  10  includes establishing fluid communication between a bodily fluid source and an inlet of the housing, at  11 . For example, in some embodiments, a user can manipulate the fluid control device to physically and/or fluidically couple the inlet to a lumen-containing device (e.g., a needle, IV, PICC line, etc.), which in turn, is in fluid communication with a patient. In other embodiments, the bodily fluid source can be a source of bodily fluid other than a patient (e.g., a reservoir, container, etc.). 
     A fluid collection device is coupled to an outlet of the housing, at  12 . The coupling of the fluid collection device to the outlet is configured to result in and/or otherwise generate a negative pressure differential within at least a portion of the fluid control device, as described in detail above with reference to the devices  100 ,  200 ,  300 , and/or  400 . In some embodiments, for example, the fluid collection device can be an evacuated container, a sample or culture bottle that defines a negative pressure, a syringe, and/or the like. The flow controller of the control device is transitioned from a first state to a second state in response to a suction force exerted by the fluid collection device to increase a volume of a sequestration chamber collectively defined by the flow controller and a portion of the housing, at  13 . For example, in some embodiments, the flow controller can be a fluid impermeable bladder or the like—similar to the flow controllers  240 ,  340 , and/or  440  described in detail above—that is disposed within the sequestration chamber. 
     The flow controller (e.g., bladder) can define any number of deformable portions configured to transition, deform, flip, and/or otherwise reconfigure in response to a suction force. In some embodiments, a first portion of the sequestration chamber can be associated with and/or at least partially defined by a first deformable portion of the flow controller and a second portion of the sequestration chamber can be associated with and/or at least partially defined by a second deformable portion of the flow controller. In some embodiments, the arrangement of the flow controller within the sequestration chamber can be such that the first portion and the second portion of the sequestration chamber are on a first side of the flow controller (e.g., fluid impermeable bladder) and a third portion of the sequestration chamber is on a second side of the flow controller opposite the first side. As described above with reference to at least the devices  200 ,  300 , and/or  400 , the arrangement of the housing, flow controller, and actuator can be such that when the actuator is in a first state and/or configuration, the inlet is in fluid communication with the first and/or second portions of the sequestration chamber (e.g., via a port similar to the first ports  217 ,  317 , and/or  417  described above) and the outlet is in fluid communication with the third portion of the sequestration chamber (e.g., via a port similar to the second ports  217 ,  317 , and/or  417  described above). As such, the third portion of the sequestration chamber can be exposed to at least a portion of the suction force generated by the fluid collection device, which in turn, is operable to transition the flow controller from its first state to its second state. 
     The first portion of the sequestration chamber receives a volume of air contained in a flow path defined between the bodily fluid source and the sequestration chamber in response to the increase in the volume of the sequestration chamber, at  14 . For example, in some embodiments, the inlet of the housing can be fluidically coupled to a needle or lumen-containing device that is, in turn, inserted into a portion of the patient. As such, the flow path can be collectively defined by, for example, a lumen of the needle or lumen-containing device, a lumen of the inlet of the housing, and a lumen of one or more flow paths, channels, openings, ports, etc. of the defined by the housing. In other words, the control device can be configured to purge the flow path of air prior to transferring bodily fluid into the sequestration chamber. 
     In some embodiments, the first portion of the sequestration chamber can be, for example, a center or central portion of the sequestration chamber. In some embodiments, the first portion of the sequestration chamber can be collectively formed by any number of regions, volumes, and/or sections (e.g., similar to the sequestration chambers  230  and/or  430  described above). In other embodiments, the first portion of the sequestration chamber can be a single and/or continuous portion (e.g., similar to the sequestration chamber  330  described above). In still other embodiments, the first portion of the sequestration chamber and the second portion of the sequestration chamber can be “inline” such that the entire sequestration chamber or substantially the entire sequestration chamber is a single and/or continuous volume. For example, in some embodiments, the sequestration chamber can have a shape and/or arrangement similar to those described in detail in U.S. Patent Publication Serial No. 2019/0076074 entitled, “Fluid Control Devices and Methods of Using the Same,” filed Sep. 12, 2018 (referred to herein as “the &#39;074 Publication”), the disclosure of which is incorporated herein by reference in its entirety. 
     The second portion of the sequestration chamber receives an initial volume of bodily fluid in response to the increase in the volume of the sequestration chamber, at  15 . More specifically, the second portion of the sequestration chamber can receive the initial volume of bodily fluid after the first portion of the sequestration chamber receives the volume of air. In some embodiments, the initial volume of bodily fluid can be a volume sufficient to substantially fill the second portion of the sequestration chamber. In other embodiments, the initial volume of bodily fluid can be a volume or amount of bodily fluid that flows into the second portion of the sequestration chamber while a negative pressure differential (e.g., resulting from the increase in volume) is below a threshold magnitude or amount. In other embodiments, bodily fluid can flow into the second portion of the sequestration chamber until pressures within the sequestration chamber and/or within the flow path between the bodily fluid source and the sequestration chamber are equalized. In still other embodiments, the initial volume can be any suitable amount or volume of bodily fluid such as any of the amounts or volumes described in detail herein. In some instances, the filling or substantial filling of the second portion of the sequestration chamber can be operable to sequester, retain, and/or fluidically lock the volume of air in the first portion of the sequestration chamber. 
     After receiving the initial volume of bodily fluid, the actuator of the device is transitioned from a first configuration to a second configuration to (1) sequester the sequestration chamber and (2) allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force, at  16 . In some embodiments, the actuator can transition from a first state to a second state to automatically sequester the initial volume of bodily fluid in the sequestration portion. In other embodiments, the actuator can transition from a first state to a second state in response to a force exerted by a user, as described above with reference to the actuators  250 ,  350 , and/or  450 . For example, in some embodiments, the actuator can be a rod or plunger that includes one or more seals or the like that can (1) fluidically isolate at least a portion of a flow path between the inlet and the sequestration chamber, (2) fluidically isolate at least a portion of a flow path between the outlet and the sequestration chamber, and (3) establish fluid communication between the inlet and the outlet to allow the subsequent volume of bodily fluid to flow therebetween. 
     With the fluid collection device fluidically coupled to the outlet of the housing, the subsequent volume of bodily fluid (e.g., one or more sample volumes) can be conveyed into the fluid collection device and used, for example, in any suitable testing such as those described herein. As described in detail above, in some instances, sequestering the initial volume of bodily fluid in the sequestration portion of the device can sequester any contaminants contained in the initial volume. Accordingly, contaminants in the subsequent volume of bodily fluid that may otherwise lead to false or inaccurate results in testing can be reduced or substantially eliminated. 
     While various embodiments have been described above, it should be understood that they have been presented by way of example only, and not limitation. Where schematics and/or embodiments described above indicate certain components arranged in certain orientations or positions, the arrangement of components may be modified. While the embodiments have been particularly shown and described, it will be understood that various changes in form and details may be made. Although various embodiments have been described as having particular features, concepts, and/or combinations of components, other embodiments are possible having any combination or sub-combination of any features, concepts, and/or components from any of the embodiments described herein. 
     In some embodiments, the specific configurations of the various components can also be varied. For example, the size and specific shape of the various components can be different from the embodiments shown, while still providing the functions as described herein. In some embodiments, varying the size and/or shape of such components may reduce an overall size of the device and/or may increase the ergonomics of the device without changing the function of the device. In some embodiments, the size and/or shape of the various components can be specifically selected for a desired or intended usage. For example, in some embodiments, a device such as those described herein can be configured for use with or on seemingly healthy adult patients. In such embodiments, the device can include a sequestration chamber that has a first volume (e.g., about 0.5 ml to about 5.0 ml). In other embodiments, a device such as those described herein can be configured for use with or on, for example, very sick patients and/or pediatric patients. In such embodiments, the device can include a sequestration chamber that has a second volume that is less than the first volume (e.g., less than about 0.5 ml). Thus, it should be understood that the size, shape, and/or arrangement of the embodiments and/or components thereof can be adapted for a given use unless the context explicitly states otherwise. 
     Any of the embodiments described herein can be used in conjunction with any suitable fluid transfer, fluid collection, and/or fluid storage device such as, for example, the fluid reservoirs described in the &#39;420 patent. In some instances, any of the embodiments described herein can be used in conjunction with any suitable transfer adapter, fluid transfer device, fluid collection device, and/or fluid storage devices such as, for example, the devices described in the &#39;783 Patent, the &#39;510 Publication, the &#39;074 Publication, and/or any of the devices described in U.S. Pat. No. 8,535,241 entitled, “Fluid Diversion Mechanism for Bodily-Fluid Sampling,” filed Oct. 22, 2012; U.S. Pat. No. 9,060,724 entitled, “Fluid Diversion Mechanism for Bodily-Fluid Sampling,” filed May 29, 2013; U.S. Pat. No. 9,155,495 entitled, “Syringe-Based Fluid Diversion Mechanism for Bodily-Fluid Sampling,” filed Dec. 2, 2013; U.S. Patent Publication No. 2016/0361006 entitled, “Devices and Methods for Syringe Based Fluid Transfer for Bodily-Fluid Sampling,” filed Jun. 23, 2016; U.S. Patent Publication No. 2018/0140240 entitled, “Systems and Methods for Sample Collection with Reduced Hemolysis,” filed Nov. 20, 2017; and/or U.S. Pat. No. 9,950,084 entitled, “Apparatus and Methods for Maintaining Sterility of a Specimen Container,” filed Sep. 6, 2016, the disclosures of which are incorporated herein by reference in their entireties. 
     While the control devices  100 ,  200 ,  300 , and/or  400  are described as transferring a bodily fluid into the device as a result of a negative pressure within a fluid collection device, in other embodiments, the devices described herein can be used with any suitable device configured to establish a negative pressure differential, suction force, and/or the like such as, for example, a syringe or pump. In other embodiments, a control device can include a pre-charged sequestration chamber, a vented sequestration chamber, a manually activated device configured to produce a negative pressure, an energy source (e.g., a chemical energy source, a kinetic energy source, and/or the like), and/or any other suitable means of defining and/or forming a pressure differential within a portion of the control device. Moreover, a control device can be coupled to such a collection device by a user (e.g., doctor, nurse, technician, physician, etc.) or can be coupled or assembled during manufacturing. In some embodiments, pre-assembling a control device and a collection device (e.g., a sample container or syringe) can, for example, force compliance with a sample procurement protocol that calls for the sequestration of an initial amount of bodily fluid prior to collecting a sample volume of bodily fluid. 
     While some of the embodiments described above include a flow controller and/or an actuator having a particular configuration and/or arrangement, in other embodiments, a fluid control device can include any suitable flow controller and/or actuator configured to selectively control a flow of bodily fluid through one or more portions of the fluid control device. For example, while some embodiments include an actuator having one or more seals arranged as an o-ring or an elastomeric over-mold, which is/are moved with the actuator and relative to a portion of the device (e.g., an inner surface of a housing or the like), in other embodiments, a fluid control device can include one or more seals having any suitable configuration. For example, in some embodiments, a fluid control device can include one or more seals arranged as an elastomeric sheet or the like that is/are fixedly coupled to a portion of the control device. In such embodiments, a portion of an actuator such as a pin or rod can extend through an opening defined in the one or more elastomeric sheets, which in turn, form a substantially fluid tight seal with an outer surface of the pin or rod. As such, at least a portion of the actuator can move relative to the one or more elastomeric sheets, which in turn, remain in a substantially fixed position relative to the portion of the control device. In some embodiments, removal of the portion of the actuator from the opening defined by the one or more elastomeric sheets can allow a flow of fluid through the opening that was otherwise occluded by the portion of the actuator. Accordingly, the one or more elastomeric sheets can function in a similar manner as any of the seals described herein. Moreover, in some embodiments, such an arrangement may, for example, reduce an amount of friction associated with forming the desired fluid tight seals, which in turn, may obviate the use of a lubricant otherwise used to facilitate the movement of the seals within the control device. 
     In some embodiments, a device and/or a flow controller can include one or more vents, membranes, members, semi-permeable barriers, and/or the like configured to at least partially control a flow of fluid through the device, flow controller, and/or actuator. For example, while portions of the sequestration chamber  230  are described above as receiving and retaining a volume of air evacuated, vented, and/or purged from the fluid flow path between the bodily fluid source and the sequestration chamber  230 , in other embodiments, a sequestration chamber  230  can include a vent or selectively permeable member configured to allow the air to exit the sequestration chamber  230 . For example, in some embodiments, a bladder or diaphragm (or portion thereof) can be formed of or from a semi-permeable material that can allow air but not bodily fluid to flow therethrough. In other embodiments, a semi-permeable material can be disposed in or along a fluid flow path between the sequestration chamber and at least one of an outlet or an inlet to selectively allow air and/or bodily fluid to flow therebetween. In some embodiments, a fluid control device can include a semi-permeable member and/or membrane that can be similar in form and/or function to the semi-permeable members and/or membranes (e.g., flow controllers) described in the &#39;074 Publication incorporated by reference hereinabove. 
     While the flow controller  240 ,  340 , and  440  are described above as being bladders configured to transition, move, flip, and/or otherwise reconfigure in response to an amount of negative pressure exerted on a surface of the bladder exceeding a threshold amount of negative pressure, in other embodiments, a fluid control device can include any suitable flow controller, actuator, semi-permeable member (e.g., air permeable and liquid impermeable), and/or the like configured to transition, move, flip, and/or otherwise reconfigure in any suitable manner in response to being exposed to a desired and/or predetermined amount of negative pressure. In other embodiments, a control device can include a bladder (or flow controller) that is configured to “flip” (e.g., relatively quickly and/or substantially uniformly transition) or configured to gradually transition (e.g., unroll, unfold, unfurl, and/or otherwise reconfigure) from the first state to the second state in response to being exposed to a negative pressure differential. In some instances, controlling a rate at which a bladder (or flow controller) is transitioned may allow for a modulation and/or control of a negative pressure differential produced within the sequestration chamber, and in turn, a magnitude of a suction force exerted within a patient&#39;s vein and/or other suitable bodily fluid source. 
     While some of the embodiments described above include a flow controller and/or actuator that physically and/or mechanically sequesters one or more portions of a fluid control device, in other embodiments, a fluid control device need not physically and/or mechanically sequester one or more portions of the fluid control device. For example, in some embodiments, an actuator such as the actuator  250  can be transitioned from a first state in which an initial volume of bodily fluid can flow from an inlet to a sequestration chamber or portion, to a second state in which (1) the sequestration chamber or portion is physically and/or mechanically sequestered and (2) the inlet is in fluid communication with an outlet of the fluid control device. In other embodiments, however, an actuator and/or any other suitable portion of a fluid control device can transition from a first state in which an initial volume of bodily fluid can flow from an inlet to a sequestration chamber or portion, to a second state in which the inlet is placed in fluid communication with the outlet without physically and/or mechanically sequestering (or isolating) the sequestration chamber or portion. When such a control device is in the second state, one or more features and/or geometries of the control device can result in a preferential flow of bodily fluid from the inlet to the outlet and the initial volume of bodily fluid can be retained in the sequestration chamber or portion without physically and/or mechanically being sequestered or isolated. 
     While the restrictor  219  is described above as modulating and/or controlling a magnitude of negative pressure applied on or through at least a portion of the device  200  (e.g., within the sequestration chamber  230  and/or otherwise on the flow controller  240 ), in other embodiments, a control device can include any suitable feature, mechanism, and/or device configured to modulate, create, and/or otherwise control one or more pressure differentials through at least a portion of the control device. For example, in some embodiments, a user can transition and/or move an actuator to change (e.g., reduce or increase) the size of one or more portions of a fluid flow path or fluid flow interface within a portion of the control device to manually modulate and/or otherwise control an amount or magnitude of negative pressure within one or more portions of a control device. 
     Although not shown, any of the devices described herein can include an opening, port, coupler, septum, Luer-Lok, gasket, valve, threaded connecter, standard fluidic interface, etc. (referred to for simplicity as a “port”) in fluid communication with the sequestration chamber. In some such embodiments, the port can be configured to couple to any suitable device, reservoir, pressure source, etc. For example, in some embodiments, the port can be configured to couple to a reservoir, which in turn, can allow a greater volume of bodily fluid to be diverted and/or transferred into the sequestration chamber. In other embodiments, the port can be coupled to a negative pressure source such as an evacuated container, a pump, a syringe, and/or the like to collect a portion or the full volume of the bodily fluid in the sequestration chamber, channel, reservoir, etc. and can use that volume of bodily fluid (e.g., the pre-sample volume) for additional clinical and/or in vitro diagnostic testing purposes. In other embodiments, the port can be configured to receive a probe, sampling tool, testing device, and/or the like that can be used to perform one or more tests (e.g., tests not sensitive to potential contamination) on the initial volume while the initial volume is disposed or sequestered in the sequestration chamber. In still other embodiments, the port can be coupled to any suitable pressure source or infusion device configured to infuse the initial volume of bodily fluid sequestered in the sequestration chamber back into the patient and/or bodily fluid source (e.g., in the case of pediatric patients, very sick patients, patients having a low blood volume, and/or the like). In other embodiments, the sequestration channel, chamber, and/or reservoir can be configured with the addition of other diagnostic testing components integrated into the chamber (e.g., a paper test) such that the initial bodily fluid is used for that test. 
     In still other embodiments, the sequestration chamber, channel, and/or reservoir can be designed, sized, and configured to be removable and compatible with testing equipment and/or specifically accessible for other types of bodily fluid tests commonly performed on patients with suspected conditions. By way of example, a patient with suspected sepsis commonly has blood samples collected for lactate testing, procalcitonin testing, and blood culture testing. All of the fluid control devices described herein can be configured such that the sequestration chamber, channel, reservoir, etc. can be removed (e.g., after receiving the initial volume of bodily fluid) and the bodily fluid contained therein can be used for these additional testing purposes before or after the subsequent sample is collected for microbial testing. 
     Although not shown, in some embodiments, a fluid control device can include one or more lumen, channels, flow paths, etc. configured to selectively allow for a “bypass” flow of bodily fluid, where an initial amount or volume of bodily fluid can flow from the inlet, through the lumen, cannel, flow path, etc. to bypass the sequestration chamber, and into the collection device. In some embodiments, the fluid control device can include an actuator having, for example, at least three states—a first in which bodily fluid can flow from the inlet to the sequestration chamber, a second in which bodily fluid can flow from the inlet to the outlet after the initial volume is sequestered in the sequestration chamber, and a third in which bodily fluid can flow from the inlet, through the bypass flow path, and to the outlet. In other embodiments, the control device can include a first actuator configured to transition the device between a first and second state, as described in detail above with reference to specific embodiments, and can include a second actuator configured to transition the device to a bypass configuration or the like. In still other embodiments, the control device can include any suitable device, feature, component, mechanism, actuator, controller, etc. configured to selectively place the fluid control device in a bypass configuration or state. 
     In some embodiments, a method of using a fluid control device such as those described herein can include the ordered steps of establishing fluid communication between a bodily fluid source (e.g., a vein of a patient or the like) and an inlet of a fluid control device. An outlet of the fluid control device is then placed in fluid communication with and/or otherwise engages a negative pressure source. Such a negative pressure source can be a sample reservoir, a syringe, an evacuated container, an intermediate transfer device, and/or the like. The fluid control device can be in a first state or operating mode when the outlet is coupled to the negative pressure source and, as such, a negative pressure differential is applied through the fluid control device that draws an initial volume of bodily fluid into a sequestration chamber of the fluid control device. For example, a negative pressure within a sample reservoir can be operable in drawing an initial volume of bodily fluid from a patient and into the sequestration chamber. Once the initial volume of bodily fluid is disposed in the sequestration chamber, the fluid control device is transitioned, either automatically or via user intervention, from the first state or operating mode to a second state or operating mode such that (1) the initial volume is sequestered in the sequestration chamber and (2) fluid communication is established between the inlet and the outlet. The sequestration of the initial volume can be such that contaminants entrained in the flow of the initial volume are likewise sequestered within the sequestration chamber. With the initial volume of bodily fluid sequestered in the sequestration chamber and with fluid communication established between the inlet and the outlet, subsequent volumes of bodily fluid that are substantially free of contamination can be collected in one or more sample reservoirs. 
     While the method of using the fluid control device is explicitly described as including the recited ordered steps, in other embodiments, the ordering of certain events and/or procedures in any of the methods or processes described herein may be modified and such modifications are in accordance with the variations of the invention. Additionally, certain events and/or procedures may be performed concurrently in a parallel process when possible, as well as performed sequentially as described above. Certain steps may be partially completed or may be omitted before proceeding to subsequent steps. For example, while the devices are described herein as transitioning from a first state to a second state in a discrete operation or the like, it should be understood that the devices described herein can be configured to automatically and/or passively transition from the first state to the second state and that such a transitioning may occur over a period of time. In other words, the transitioning from the first state to the second state may, in some instances, be relatively gradual such that as a last portion of the initial volume of bodily fluid is being transferred into the sequestration chamber, the housing begins to transition from the first state to the second state. In some instances, the rate of change when transitioning from the first state to the second state can be selectively controlled to achieve one or more desired characteristics associated with the transition. Moreover, in some such instances, the inflow of the last portion of the initial volume can limit and/or substantially prevent bodily fluid already disposed in the sequestration chamber from escaping therefrom. Accordingly, while the transitioning from the first state to the second state may occur over a given amount of time, the sequestration chamber can nonetheless sequester the volume of bodily fluid disposed therein.