Patent Publication Number: US-2007111230-A1

Title: Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport

Description:
RELATED APPLICATIONS  
      This application is a divisional of U.S. application Ser. No. 10/627,476, filed Jul. 25, 2003, which is a continuation of U.S. application Ser. No. 09/602,787, filed Jun. 23, 2000, which, in turn, claims priority to prior filed U.S. Provisional Patent Application Ser. No. 60/141031, filed Jun. 25, 1999. This application also claims priority to German Patent Application No. 19931454.3, filed Jul. 8, 1999, German Patent Application No. 19931478.0, filed Jul. 8, 1999, German Patent Application No. 19931563.9, filed Jul. 8, 1999, German Patent Application No. 19932122.1, filed Jul. 9, 1999, German Patent Application No. 19932124.8, filed Jul. 9, 1999, German Patent Application No. 19932125.6, filed Jul. 9, 1999, German Patent Application No. 19932128.0, filed Jul. 9, 1999, German Patent Application No. 19932180.9, filed Jul. 9, 1999, German Patent Application No. 19932182.5, filed Jul. 9, 1999, German Patent Application No. 19932190.6, filed Jul. 9, 1999, German Patent Application No. 19932191.4, filed Jul. 9, 1999, German Patent Application No. 19932209.0, filed Jul. 9, 1999, German Patent Application No. 19932212.0, filed Jul. 9, 1999, German Patent Application No. 19932227.9, filed Jul. 9, 1999, German Patent Application No. 19932228.7, filed Jul. 9, 1999, German Patent Application No. 19932229.5, filed Jul. 9, 1999, German Patent Application No. 19932230.9, filed Jul. 9, 1999, German Patent Application No. 19932927.3, filed Jul. 14, 1999, German Patent Application No. 19933005.0, filed Jul. 14, 1999, German Patent Application No. 19933006.9, filed Jul. 14, 1999, German Patent Application No. 19940764.9, filed Aug. 27, 1999, German Patent Application No. 19940765.7, filed Aug. 27, 1999, German Patent Application No. 19940766.5, filed Aug. 27, 1999, German Patent Application No. 19940830.0, filed Aug. 27, 1999, German Patent Application No. 19940831.9, filed Aug. 27, 1999, German Patent Application No. 19940832.7, filed Aug. 27, 1999, German Patent Application No. 19940833.5, filed Aug. 27, 1999, German Patent Application No. 19941378.9 filed Aug. 31, 1999, German Patent Application No. 19941379.7, filed Aug. 31, 1999, German Patent Application No. 19941395.9, filed Aug. 31, 1999, German Patent Application No. 19942077.7, filed Sep. 3, 1999, German Patent Application No. 19942078.5, filed September 3, 1999, German Patent Application No. 19942079.3, filed Sep. 3, 1999, and German Patent Application No. 19942088.2, filed Sep. 3, 1999. The entire contents of all of the above referenced applications are hereby expressly incorporated herein by this reference.  
    
    
     INCORPORATION OF MATERIAL SUBMITTED ON COMPACT DISCS  
      This application incorporates herein by reference the material contained on the compact discs submitted herewith as part of this application. Specifically, the file “seqlistcorr2” (2.43 MB) contained on each of Copy 1, Copy 2 and the CRF copy of the Sequence Listing is hereby incorporated herein by reference. This file was created on Jul. 31, 2006. In addition, the files “Appendix A” (399 KB) and “Appendix B” (140 KB) contained on each of the compact disks entitled “Appendices Copy 1” and “Appendices Copy 2” are hereby incorporated herein by reference. Each of these files were created on Jul. 31, 2006.  
     BACKGROUND OF THE INVENTION  
      Certain products and by-products of naturally-occurring metabolic processes in cells have utility in a wide array of industries, including the food, feed, cosmetics, and pharmaceutical industries. These molecules, collectively termed ‘fine chemicals’, include organic acids, both proteinogenic and non-proteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and enzymes. Their production is most conveniently performed through the large-scale culture of bacteria developed to produce and secrete large quantities of one or more desired molecules. One particularly useful organism for this purpose is  Corynebacterium glutamicum , a gram positive, nonpathogenic bacterium. Through strain selection, a number of mutant strains have been developed which produce an array of desirable compounds. However, selection of strains improved for the production of a particular molecule is a time-consuming and difficult process.  
     SUMMARY OF THE INVENTION  
      The invention provides novel bacterial nucleic acid molecules which have a variety of uses. These uses include the identification of microorganisms which can be used to produce fine chemicals, the modulation of fine chemical production in  C. glutamicum  or related bacteria, the typing or identification of  C. glutamicum  or related bacteria, as reference points for mapping the  C. glutamicum  genome, and as markers for transformation. These novel nucleic acid molecules encode proteins, referred to herein as membrane construction and membrane transport (MCT) proteins.  
       C. glutamicum  is a gram positive, aerobic bacterium which is commonly used in industry for the large-scale production of a variety of fine chemicals, and also for the degradation of hydrocarbons (such as in petroleum spills) and for the oxidation of terpenoids. The MCT nucleic acid molecules of the invention, therefore, can be used to identify microorganisms which can be used to produce fine chemicals, e.g., by fermentation processes. Modulation of the expression of the MCT nucleic acids of the invention, or modification of the sequence of the MCT nucleic acid molecules of the invention, can be used to modulate the production of one or more fine chemicals from a microorganism (e.g., to improve the yield or production of one or more fine chemicals from a Corynebacterium or Brevibacterium species).  
      The MCT nucleic acids of the invention may also be used to identify an organism as being  Corynebacterium glutamicum  or a close relative thereof, or to identify the presence of  C. glutamicum  or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of  C. glutamicum  genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a  C. glutamicum  gene which is unique to this organism, one can ascertain whether this organism is present. Although  Corynebacterium glutamicum  itself is nonpathogenic, it is related to species pathogenic in humans, such as  Corynebacterium diphtheriae  (the causative agent of diphtheria); the detection of such organisms is of significant clinical relevance.  
      The MCT nucleic acid molecules of the invention may also serve as reference points for mapping of the  C. glutamicum  genome, or of genomes of related organisms. Similarly, these molecules, or variants or portions thereof, may serve as markers for genetically engineered Corynebacterium or Brevibacterium species. e.g.e.g. The MCT proteins encoded by the novel nucleic acid molecules of the invention are capable of, for example, performing a function involved in the metabolism (e.g., the biosynthesis or degradation) of compounds necessary for membrane biosynthesis, or of assisting in the transmembrane transport of one or more compounds either into or out of the cell. Given the availability of cloning vectors for use in  Corynebacterium glutamicum,  such as those disclosed in Sinskey et al., U.S. Pat. No. 4,649,119, and techniques for genetic manipulation of  C. glutamicum  and the related  Brevibacterium  species (e.g.,  lactofermentum ) (Yoshihama et al,  J. Bacteriol.  162: 591-597 (1985); Katsumata et al.,  J. Bacteriol.  159: 306-311 (1984); and Santamaria et al.,  J. Gen. Microbiol.  130: 2237-2246 (1984)), the nucleic acid molecules of the invention may be utilized in the genetic engineering of this organism to make it a better or more efficient producer of one or more fine chemicals. This improved production or efficiency of production of a fine chemical may be due to a direct effect of manipulation of a gene of the invention, or it may be due to an indirect effect of such manipulation.  
      There are a number of mechanisms by which the alteration of an MCT protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a  C. glutamicum  strain incorporating such an altered protein. Those MCT proteins involved in the export of fine chemical molecules from the cell may be increased in number or activity such that greater quantities of these compounds are secreted to the extracellular medium, from which they are more readily recovered. Similarly, those MCT proteins involved in the import of nutrients necessary for the biosynthesis of one or more fine chemicals (e.g., phosphate, sulfate, nitrogen compounds, etc.) may be increased in number or activity such that these precursors, cofactors, or intermediate compounds are increased in concentration within the cell. Further, fatty acids and lipids themselves are desirable fine chemicals; by optimizing the activity or increasing the number of one or more MCT proteins of the invention which participate in the biosynthesis of these compounds, or by impairing the activity of one or more MCT proteins which are involved in the degradation of these compounds, it may be possible to increase the yield, production, and/or efficiency of production of fatty acid and lipid molecules from  C. glutamicum.    
      The mutagenesis of one or more MCT genes of the invention may also result in MCT proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from  C. glutamicum . For example, MCT proteins of the invention involved in the export of waste products may be increased in number or activity such that the normal metabolic wastes of the cell (possibly increased in quantity due to the overproduction of the desired fine chemical) are efficiently exported before they are able to damage nucleotides and proteins within the cell (which would decrease the viability of the cell) or to interfere with fine chemical biosynthetic pathways (which would decrease the yield, production, or efficiency of production of the desired fine chemical). Further, the relatively large intracellular quantities of the desired fine chemical may in itself be toxic to the cell, so by increasing the activity or number of transporters able to export this compound from the cell, one may increase the viability of the cell in culture, in turn leading to a greater number of cells in the culture producing the desired fine chemical. The MCT proteins of the invention may also be manipulated such that the relative amounts of different lipid and fatty acid molecules are produced. This may have a profound effect on the lipid composition of the membrane of the cell. Since each type of lipid has different physical properties, an alteration in the lipid composition of a membrane may significantly alter membrane fluidity. Changes in membrane fluidity can impact the transport of molecules across the membrane, as well as the integrity of the cell, both of which have a profound effect on the production of fine chemicals from  C. glutamicum  in large-scale fermentative culture.  
      The invention provides novel nucleic acid molecules which encode proteins, referred to herein as MCT proteins, which are capable of, for example, participating in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum,  or in the transport of molecules across these membranes. Nucleic acid molecules encoding an MCT protein are referred to herein as MCT nucleic acid molecules. In a preferred embodiment, the MCT protein participates in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes. Examples of such proteins include those encoded by the genes set forth in Table 1.  
      Accordingly, one aspect of the invention pertains to isolated nucleic acid molecules (e.g., cDNAs, DNAs, or RNAs) comprising a nucleotide sequence encoding an MCT protein or biologically active portions thereof, as well as nucleic acid fragments suitable as primers or hybridization probes for the detection or amplification of MCT-encoding nucleic acid (e.g., DNA or mRNA). In particularly preferred embodiments, the isolated nucleic acid molecule comprises one of the nucleotide sequences set forth in Appendix A or the coding region or a complement thereof of one of these nucleotide sequences. In other particularly preferred embodiments, the isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes to or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80% or 90%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence set forth in Appendix A, or a portion thereof. In other preferred embodiments, the isolated nucleic acid molecule encodes one of the amino acid sequences set forth in Appendix B. The preferred MCT proteins of the present invention also preferably possess at least one of the MCT activities described herein.  
      In another embodiment, the isolated nucleic acid molecule encodes a protein or portion thereof wherein the protein or portion thereof includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of Appendix B, e.g., sufficiently homologous to an amino acid sequence of Appendix B such that the protein or portion thereof maintains an MCT activity. Preferably, the protein or portion thereof encoded by the nucleic acid molecule maintains the ability to participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes. In one embodiment, the protein encoded by the nucleic acid molecule is at least about 50%, preferably at least about 60%, and more preferably at least about 70%, 80%, or 90% and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an amino acid sequence of Appendix B (e.g., an entire amino acid sequence selected from those sequences set forth in Appendix B). In another preferred embodiment, the protein is a full length  C. glutamicum  protein which is substantially homologous to an entire amino acid sequence of Appendix B (encoded by an open reading frame shown in Appendix A).  
      In another preferred embodiment, the isolated nucleic acid molecule is derived from  C. glutamicum  and encodes a protein (e.g., an MCT fusion protein) which includes a biologically active domain which is at least about 50% or more homologous to one of the amino acid sequences of Appendix B and is able to participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes, or has one or more of the activities set forth in Table 1, and which also includes heterologous nucleic acid sequences encoding a heterologous polypeptide or regulatory regions.  
      In another embodiment, the isolated nucleic acid molecule is at least 15 nucleotides in length and hybridizes under stringent conditions to a nucleic acid molecule comprising a nucleotide sequence of Appendix A. Preferably, the isolated nucleic acid molecule corresponds to a naturally-occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes a naturally-occurring  C. glutamicum  MCT protein, or a biologically active portion thereof.  
      Another aspect of the invention pertains to vectors, e.g., recombinant expression vectors, containing the nucleic acid molecules of the invention, and host cells into which such vectors have been introduced. In one embodiment, such a host cell is used to produce an MCT protein by culturing the host cell in a suitable medium. The MCT protein can be then isolated from the medium or the host cell.  
      Yet another aspect of the invention pertains to a genetically altered microorganism in which an MCT gene has been introduced or altered. In one embodiment, the genome of the microorganism has been altered by introduction of a nucleic acid molecule of the invention encoding wild-type or mutated MCT sequence as a transgene. In another embodiment, an endogenous MCT gene within the genome of the microorganism has been altered, e.g., functionally disrupted, by homologous recombination with an altered MCT gene. In another embodiment, an endogenous or introduced MCT gene in a microorganism has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional MCT protein. In still another embodiment, one or more of the regulatory regions (e.g., a promoter, repressor, or inducer) of an MCT gene in a microorganism has been altered (e.g., by deletion, truncation, inversion, or point mutation) such that the expression of the MCT gene is modulated. In a preferred embodiment, the microorganism belongs to the genus  Corynebacterium  or  Brevibacterium,  with  Corynebacterium glutamicum  being particularly preferred. In a preferred embodiment, the microorganism is also utilized for the production of a desired compound, such as an amino acid, with lysine being particularly preferred.  
      In another aspect, the invention provides a method of identifying the presence or activity of  Cornyebacterium diphtheriae  in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention (e.g., the sequences set forth in Appendix A or Appendix B) in a subject, thereby detecting the presence or activity of  Corynebacterium diphtheriae  in the subject.  
      Still another aspect of the invention pertains to an isolated MCT protein or a portion, e.g., a biologically active portion, thereof. In a preferred embodiment, the isolated MCT protein or portion thereof can participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes. In another preferred embodiment, the isolated MCT protein or portion thereof is sufficiently homologous to an amino acid sequence of Appendix B such that the protein or portion thereof maintains the ability to participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes.  
      The invention also provides an isolated preparation of an MCT protein. In preferred embodiments, the MCT protein comprises an amino acid sequence of Appendix B. In another preferred embodiment, the invention pertains to an isolated full length protein which is substantially homologous to an entire amino acid sequence of Appendix B (encoded by an open reading frame set forth in Appendix A). In yet another embodiment, the protein is at least about 50%, preferably at least about 60%, and more preferably at least about 70%, 80%, or 90%, and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an entire amino acid sequence of Appendix B. In other embodiments, the isolated MCT protein comprises an amino acid sequence which is at least about 50% or more homologous to one of the amino acid sequences of Appendix B and is able to participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes, or has one or more of the activities set forth in Table 1.  
      Alternatively, the isolated MCT protein can comprise an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80%, or 90%, and even more preferably at least about 95%, 96%, 97%, 98,%, or 99% or more homologous, to a nucleotide sequence of Appendix B. It is also preferred that the preferred forms of MCT proteins also have one or more of the MCT bioactivities described herein.  
      The MCT polypeptide, or a biologically active portion thereof, can be operatively linked to a non-MCT polypeptide to form a fusion protein. In preferred embodiments, this fusion protein has an activity which differs from that of the MCT protein alone. In other preferred embodiments, this fusion protein participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum,  or in the transport of molecules across these membranes. In particularly preferred embodiments, integration of this fusion protein into a host cell modulates production of a desired compound from the cell.  
      In another aspect, the invention provides methods for screening molecules which modulate the activity of an MCT protein, either by interacting with the protein itself or a substrate or binding partner of the MCT protein, or by modulating the transcription or translation of an MCT nucleic acid molecule of the invention.  
      Another aspect of the invention pertains to a method for producing a fine chemical. This method involves the culturing of a cell containing a vector directing the expression of an MCT nucleic acid molecule of the invention, such that a fine chemical is produced. In a preferred embodiment, this method further includes the step of obtaining a cell containing such a vector, in which a cell is transfected with a vector directing the expression of an MCT nucleic acid. In another preferred embodiment, this method further includes the step of recovering the fine chemical from the culture. In a particularly preferred embodiment, the cell is from the genus  Corynebacterium  or  Brevibacterium,  or is selected from those strains set forth in Table 3.  
      Another aspect of the invention pertains to methods for modulating production of a molecule from a microorganism. Such methods include contacting the cell with an agent which modulates MCT protein activity or MCT nucleic acid expression such that a cell associated activity is altered relative to this same activity in the absence of the agent. In a preferred embodiment, the cell is modulated for one or more  C. glutamicum  metabolic pathways for cell membrane components or is modulated for the transport of compounds across such membranes, such that the yields or rate of production of a desired fine chemical by this microorganism is improved. The agent which modulates MCT protein activity can be an agent which stimulates MCT protein activity or MCT nucleic acid expression. Examples of agents which stimulate MCT protein activity or MCT nucleic acid expression include small molecules, active MCT proteins, and nucleic acids encoding MCT proteins that have been introduced into the cell. Examples of agents which inhibit MCT activity or expression include small molecules and antisense MCT nucleic acid molecules.  
      Another aspect of the invention pertains to methods for modulating yields of a desired compound from a cell, involving the introduction of a wild-type or mutant MCT gene into a cell, either maintained on a separate plasmid or integrated into the genome of the host cell. If integrated into the genome, such integration can be random, or it can take place by homologous recombination such that the native gene is replaced by the introduced copy, causing the production of the desired compound from the cell to be modulated. In a preferred embodiment, said yields are increased. In another preferred embodiment, said chemical is a fine chemical. In a particularly preferred embodiment, said fine chemical is an amino acid. In especially preferred embodiments, said amino acid is L-lysine. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION  
      The present invention provides MCT nucleic acid and protein molecules which are involved in the metabolism of cellular membrane components in  C. glutamicum  or in the transport of compounds across such membranes. The molecules of the invention may be utilized in the modulation of production of fine chemicals from microorganisms, such as  C. glutamicum , either directly (e.g., where overexpression or optimization of a fatty acid biosynthesis protein has a direct impact on the yield, production, and/or efficiency of production of the fatty acid from modified  C. glutamicum ), or may have an indirect impact which nonetheless results in an increase of yield, production, and/or efficiency of production of the desired compound (e.g., where modulation of the metabolism of cell membrane components results in alterations in the yield, production, and/or efficiency of production or the composition of the cell membrane, which in turn may impact the production of one or more fine chemicals). Aspects of the invention are further explicated below.  
      I. Fine Chemicals  
      The term ‘fine chemical’ is art-recognized and includes molecules produced by an organism which have applications in various industries, such as, but not limited to, the pharmaceutical, agriculture, and cosmetics industries. Such compounds include organic acids, such as tartaric acid, itaconic acid, and diaminopimelic acid, both proteinogenic and non-proteinogenic amino acids, purine and pyrimidine bases, nucleosides, and nucleotides (as described e.g. in Kuninaka, A. (1996) Nucleotides and related compounds, p. 561-612, in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, and references contained therein), lipids, both saturated and unsaturated fatty acids (e.g., arachidonic acid), diols (e.g., propane diol, and butane diol), carbohydrates (e.g, hyaluronic acid and trehalose), aromatic compounds (e.g, aromatic amines, vanillin, and indigo), vitamins and cofactors (as described in Ullmann&#39;s Encyclopedia of Industrial Chemistry, vol. A27, “Vitamins”, p. 443-613 (1996) VCH: Weinheim and references therein; and Ong, A.S., Niki, E. &amp; Packer, L. (1995) “Nutrition, Lipids, Health, and Disease” Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research—Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)), enzymes, polyketides (Cane et al. (1998)  Science  282: 63-68), and all other chemicals described in Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and references therein. The metabolism and uses of certain of these fine chemicals are further explicated below.  
      A. Amino Acid Metabolism and Uses  
      Amino acids comprise the basic structural units of all proteins, and as such are essential for normal cellular functioning in all organisms. The term “amino acid” is art-recognized. The proteinogenic amino acids, of which there are 20 species, serve as structural units for proteins, in which they are linked by peptide bonds, while the nonproteinogenic amino acids (hundreds of which are known) are not normally found in proteins (see Ulmann&#39;s Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: Weinheim (1985)). Amino acids may be in the D- or L-optical configuration, though L-amino acids are generally the only type found in naturally-occurring proteins. Biosynthetic and degradative pathways of each of the 20 proteinogenic amino acids have been well characterized in both prokaryotic and eukaryotic cells (see, for example, Stryer, L. Biochemistry, 3 rd  edition, pages 578-590 (1988)). The ‘essential’ amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine), so named because they are generally a nutritional requirement due to the complexity of their biosyntheses, are readily converted by simple biosynthetic pathways to the remaining 11 ‘nonessential’ amino acids (alanine, arginine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine, and tyrosine). Higher animals do retain the ability to synthesize some of these amino acids, but the essential amino acids must be supplied from the diet in order for normal protein synthesis to occur.  
      Aside from their function in protein biosynthesis, these amino acids are interesting chemicals in their own right, and many have been found to have various applications in the food, feed, chemical, cosmetics, agriculture, and pharmaceutical industries. Lysine is an important amino acid in the nutrition not only of humans, but also of monogastric animals such as poultry and swine. Glutamate is most commonly used as a flavor additive (mono-sodium glutamate, MSG) and is widely used throughout the food industry, as are aspartate, phenylalanine, glycine, and cysteine. Glycine, L-methionine and tryptophan are all utilized in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are of use in both the pharmaceutical and cosmetics industries. Threonine, tryptophan, and D/ L-methionine are common feed additives. (Leuchtenberger, W. (1996) Amino aids—technical production and use, p. 466-502 in Rehm et al. (eds.) Biotechnology vol. 6, chapter 14a, VCH: Weinheim). Additionally, these amino acids have been found to be useful as precursors for the synthesis of synthetic amino acids and proteins, such as N-acetylcysteine, S-carboxymethyl-L-cysteine, (S)-5-hydroxytryptophan, and others described in Ulmann&#39;s Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: Weinheim, 1985.  
      The biosynthesis of these natural amino acids in organisms capable of producing them, such as bacteria, has been well characterized (for review of bacterial amino acid biosynthesis and regulation thereof, see Umbarger, H. E.(1978)  Ann. Rev. Biochem.  47: 533-606). Glutamate is synthesized by the reductive amination of α-ketoglutarate, an intermediate in the citric acid cycle. Glutamine, proline, and arginine are each subsequently produced from glutamate. The biosynthesis of serine is a three-step process beginning with 3-phosphoglycerate (an intermediate in glycolysis), and resulting in this amino acid after oxidation, transamination, and hydrolysis steps. Both cysteine and glycine are produced from serine; the former by the condensation of homocysteine with serine, and the latter by the transferal of the side-chain β-carbon atom to tetrahydrofolate, in a reaction catalyzed by serine transhydroxymethylase. Phenylalanine, and tyrosine are synthesized from the glycolytic and pentose phosphate pathway precursors erythrose 4-phosphate and phosphoenolpyruvate in a 9-step biosynthetic pathway that differ only at the final two steps after synthesis of prephenate. Tryptophan is also produced from these two initial molecules, but its synthesis is an 11-step pathway. Tyrosine may also be synthesized from phenylalanine, in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine, and leucine are all biosynthetic products of pyruvate, the final product of glycolysis. Aspartate is formed from oxaloacetate, an intermediate of the citric acid cycle. Asparagine, methionine, threonine, and lysine are each produced by the conversion of aspartate. Isoleucine is formed from threonine. A complex 9-step pathway results in the production of histidine from 5-phosphoribosyl-1-pyrophosphate, an activated sugar.  
      Amino acids in excess of the protein synthesis needs of the cell cannot be stored, and are instead degraded to provide intermediates for the major metabolic pathways of the cell (for review see Stryer, L. Biochemistry  3   rd  ed. Ch. 21 “Amino Acid Degradation and the Urea Cycle” p. 495-516 (1988)). Although the cell is able to convert unwanted amino acids into useful metabolic intermediates, amino acid production is costly in terms of energy, precursor molecules, and the enzymes necessary to synthesize them. Thus it is not surprising that amino acid biosynthesis is regulated by feedback inhibition, in which the presence of a particular amino acid serves to slow or entirely stop its own production (for overview of feedback mechanisms in amino acid biosynthetic pathways, see Stryer, L. Biochemistry, 3 rd  ed. Ch. 24: “Biosynthesis of Amino Acids and Heme” p. 575-600 (1988)). Thus, the output of any particular amino acid is limited by the amount of that amino acid present in the cell.  
      B. Vitamin, Cofactor, and Nutraceutical Metabolism and Uses  
      Vitamins, cofactors, and nutraceuticals comprise another group of molecules which the higher animals have lost the ability to synthesize and so must ingest, although they are readily synthesized by other organisms such as bacteria. These molecules are either bioactive substances themselves, or are precursors of biologically active substances which may serve as electron carriers or intermediates in a variety of metabolic pathways. Aside from their nutritive value, these compounds also have significant industrial value as coloring agents, antioxidants, and catalysts or other processing aids. (For an overview of the structure, activity, and industrial applications of these compounds, see, for example, Ullman&#39;s Encyclopedia of Industrial Chemistry, “Vitamins” vol. A27, p. 443-613, VCH: Weinheim, 1996.) The term “vitamin” is art-recognized, and includes nutrients which are required by an organism for normal functioning, but which that organism cannot synthesize by itself. The group of vitamins may encompass cofactors and nutraceutical compounds. The language “cofactor” includes nonproteinaceous compounds required for a normal enzymatic activity to occur. Such compounds may be organic or inorganic; the cofactor molecules of the invention are preferably organic. The term “nutraceutical” includes dietary supplements having health benefits in plants and animals, particularly humans. Examples of such molecules are vitamins, antioxidants, and also certain lipids (e.g., polyunsaturated fatty acids).  
      The biosynthesis of these molecules in organisms capable of producing them, such as bacteria, has been largely characterized (Ullman&#39;s Encyclopedia of Industrial Chemistry, “Vitamins” vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley &amp; Sons; Ong, A.S., Niki, E. &amp; Packer, L. (1995) “Nutrition, Lipids, Health, and Disease” Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research—Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).  
      Thiamin (vitamin B 1 ) is produced by the chemical coupling of pyrimidine and thiazole moieties. Riboflavin (vitamin B 2 ) is synthesized from guanosine-5′-triphosphate (GTP) and ribose-5′-phosphate. Riboflavin, in turn, is utilized for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The family of compounds collectively termed ‘vitamin B 6 ’ (e.g., pyridoxine, pyridoxamine, pyridoxa-5′-phosphate, and the commercially used pyridoxin hydrochloride) are all derivatives of the common structural unit, 5-hydroxy-6-methylpyridine. Pantothenate (pantothenic acid, (R)-(+)-N-(2,4-dihydroxy-3,3-dimethyl-1-oxobutyl)-β-alanine) can be produced either by chemical synthesis or by fermentation. The final steps in pantothenate biosynthesis consist of the ATP-driven condensation of β-alanine and pantoic acid. The enzymes responsible for the biosynthesis steps for the conversion to pantoic acid, to β-alanine and for the condensation to panthotenic acid are known. The metabolically active form of pantothenate is Coenzyme A, for which the biosynthesis proceeds in 5 enzymatic steps. Pantothenate, pyridoxal-5′-phosphate, cysteine and ATP are the precursors of Coenzyme A. These enzymes not only catalyze the formation of panthothante, but also the production of (R)-pantoic acid, (R)-pantolacton, (R)-panthenol (provitamin B 5 ), pantetheine (and its derivatives) and coenzyme A.  
      Biotin biosynthesis from the precursor molecule pimeloyl-CoA in microorganisms has been studied in detail and several of the genes involved have been identified. Many of the corresponding proteins have been found to also be involved in Fe-cluster synthesis and are members of the nifS class of proteins. Lipoic acid is derived from octanoic acid, and serves as a coenzyme in energy metabolism, where it becomes part of the pyruvate dehydrogenase complex and the α-ketoglutarate dehydrogenase complex. The folates are a group of substances which are all derivatives of folic acid, which is turn is derived from L-glutamic acid, p-amino-benzoic acid and 6-methylpterin. The biosynthesis of folic acid and its derivatives, starting from the metabolism intermediates guanosine-5′-triphosphate (GTP), L-glutamic acid and p-amino-benzoic acid has been studied in detail in certain microorganisms.  
      Corrinoids (such as the cobalamines and particularly vitamin B 12 ) and porphyrines belong to a group of chemicals characterized by a tetrapyrole ring system. The biosynthesis of vitamin B 12  is sufficiently complex that it has not yet been completely characterized, but many of the enzymes and substrates involved are now known. Nicotinic acid (nicotinate), and nicotinamide are pyridine derivatives which are also termed ‘niacin’. Niacin is the precursor of the important coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate) and their reduced forms.  
      The large-scale production of these compounds has largely relied on cell-free chemical syntheses, though some of these chemicals have also been produced by large-scale culture of microorganisms, such as riboflavin, Vitamin B 6 , pantothenate, and biotin. Only Vitamin B 12  is produced solely by fermentation, due to the complexity of its synthesis. In vitro methodologies require significant inputs of materials and time, often at great cost.  
      C. Purine, Pyrimidine, Nucleoside and Nucleotide Metabolism and Uses  
      Purine and pyrimidine metabolism genes and their corresponding proteins are important targets for the therapy of tumor diseases and viral infections. The language “purine” or “pyrimidine” includes the nitrogenous bases which are constituents of nucleic acids, co-enzymes, and nucleotides. The term “nucleotide” includes the basic structural units of nucleic acid molecules, which are comprised of a nitrogenous base, a pentose sugar (in the case of RNA, the sugar is ribose; in the case of DNA, the sugar is D-deoxyribose), and phosphoric acid. The language “nucleoside” includes molecules which serve as precursors to nucleotides, but which are lacking the phosphoric acid moiety that nucleotides possess. By inhibiting the biosynthesis of these molecules, or their mobilization to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; by inhibiting this activity in a fashion targeted to cancerous cells, the ability of tumor cells to divide and replicate may be inhibited. Additionally, there are nucleotides which do not form nucleic acid molecules, but rather serve as energy stores (i.e., AMP) or as coenzymes (i.e., FAD and NAD).  
      Several publications have described the use of these chemicals for these medical indications, by influencing purine and/or pyrimidine metabolism (e.g Christopherson, R. I. and Lyons, S. D. (1990) “Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents.”  Med. Res. Reviews  10: 505-548). Studies of enzymes involved in purine and pyrimidine metabolism have been focused on the development of new drugs which can be used, for example, as immunosuppressants or anti-proliferants (Smith, J. L., (1995) “Enzymes in nucleotide synthesis.”  Curr. Opin. Struct. Biol.  5: 752-757; (1995)  Biochem Soc. Transact.  23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides have other utilities: as intermediates in the biosynthesis of several fine chemicals (e.g., thiamine, S-adenosyl-methionine, folates, or riboflavin), as energy carriers for the cell (e.g., ATP or GTP), and for chemicals themselves, commonly used as flavor enhancers (e.g., IMP or GMP) or for several medicinal applications (see, for example, Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, p. 561-612). Also, enzymes involved in purine, pyrimidine, nucleoside, or nucleotide metabolism are increasingly serving as targets against which chemicals for crop protection, including fungicides, herbicides and insecticides, are developed.  
      The metabolism of these compounds in bacteria has been characterized (for reviews see, for example, Zalkin, H. and Dixon, J. E. (1992) “de novo purine nucleotide biosynthesis”, in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press:, p. 259-287; and Michal, G. (I 999) “Nucleotides and Nucleosides”, Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York). Purine metabolism has been the subject of intensive research, and is essential to the normal functioning of the cell. Impaired purine metabolism in higher animals can cause severe disease, such as gout. Purine nucleotides are synthesized from ribose-5-phosphate, in a series of steps through the intermediate compound inosine-5′-phosphate (IMP), resulting in the production of guanosine-5′-monophosphate (GMP) or adenosine-5′-monophosphate (AMP), from which the triphosphate forms utilized as nucleotides are readily formed. These compounds are also utilized as energy stores, so their degradation provides energy for many different biochemical processes in the cell. Pyrimidine biosynthesis proceeds by the formation of uridine-5′-monophosphate (UMP) from ribose-5-phosphate. UMP, in turn, is converted to cytidine-5′-triphosphate (CTP). The deoxy- forms of all of these nucleotides are produced in a one step reduction reaction from the diphosphate ribose form of the nucleotide to the diphosphate deoxyribose form of the nucleotide. Upon phosphorylation, these molecules are able to participate in DNA synthesis.  
      D. Trehalose Metabolism and Uses  
      Trehalose consists of two glucose molecules, bound in α, α-1,1 linkage. It is commonly used in the food industry as a sweetener, an additive for dried or frozen foods, and in beverages. However, it also has applications in the pharmaceutical, cosmetics and biotechnology industries (see, for example, Nishimoto et al., (1998) U.S. Pat. No. 5,759,610; Singer, M. A. and Lindquist, S. (1998)  Trends Biotech.  16: 460-467; Paiva, C. L. A. and Panek, A. D. (1996)  Biotech. Ann. Rev.  2: 293-314; and Shiosaka, M. (1997) J. Japan 172: 97-102). Trehalose is produced by enzymes from many microorganisms and is naturally released into the surrounding medium, from which it can be collected using methods known in the art.  
      II. Membrane Biosynthesis and Transmembrane Transport  
      Cellular membranes serve a variety of functions in a cell. First and foremost, a membrane differentiates the contents of a cell from the surrounding environment, thus giving integrity to the cell. Membranes may also serve as barriers to the influx of hazardous or unwanted compounds, and also to the efflux of desired compounds. Cellular membranes are by nature impervious to the unfacilitated diffusion of hydrophilic compounds such as proteins, water molecules and ions due to their structure: a bilayer of lipid molecules in which the polar head groups face outwards (towards the exterior and interior of the cell, respectively) and the nonpolar tails face inwards at the center of the bilayer, forming a hydrophobic core (for a general review of membrane structure and function, see Gennis, R. B. (1989) Biomembranes, Molecular Structure and Function, Springer: Heidelberg). This barrier enables cells to maintain a relatively higher concentration of desired compounds and a relatively lower concentration of undesired compounds than are contained within the surrounding medium, since the diffusion of these compounds is effectively blocked by the membrane. However, the membrane also presents an effective barrier to the import of desired compounds and the export of waste molecules. To overcome this difficulty, cellular membranes incorporate many kinds of transporter proteins which are able to facilitate the transmembrane transport of different kinds of compounds. There are two general classes of these transport proteins: pores or channels and transporters. The former are integral membrane proteins, sometimes complexes of proteins, which form a regulated hole through the membrane. This regulation, or ‘gating’ is generally specific to the molecules to be transported by the pore or channel, rendering these transmembrane constructs selectively permeable to a specific class of substrates; for example, a potassium channel is constructed such that only ions having a like charge and size to that of potassium may pass through. Channel and pore proteins tend to have discrete hydrophobic and hydrophilic domains, such that the hydrophobic face of the protein may associate with the interior of the membrane while the hydrophilic face lines the interior of the channel, thus providing a sheltered hydrophilic environment through which the selected hydrophilic molecule may pass. Many such pores/channels are known in the art, including those for potassium, calcium, sodium, and chloride ions.  
      This pore and channel-mediated system of facilitated diffusion is limited to very small molecules, such as ions, because pores or channels large enough to permit the passage of whole proteins by facilitated diffusion would be unable to prevent the passage of smaller hydrophilic molecules as well. Transport of molecules by this process is sometimes termed ‘facilitated diffusion’ since the driving force of a concentration gradient is required for the transport to occur. Permeases also permit facilitated diffusion of larger molecules, such as glucose or other sugars, into the cell when the concentration of these molecules on one side of the membrane is greater than that on the other (also called ‘uniport’). In contrast to pores or channels, these integral membrane proteins (often having between 6-14 membrane-spanning β-helices) do not form open channels through the membrane, but rather bind to the target molecule at the surface of the membrane and then undergo a conformational shift such that the target molecule is released on the opposite side of the membrane.  
      However, cells frequently require the import or export of molecules against the existing concentration gradient (‘active transport’), a situation in which facilitated diffusion cannot occur. There are two general mechanisms used by cells for such membrane transport: symport or antiport, and energy-coupled transport such as that mediated by the ABC transporters. Symport and antiport systems couple the movement of two different molecules across the membrane (via permeases having two separate binding sites for the two different molecules); in symport, both molecules are transported in the same direction, while in antiport, one molecule is imported while the other is exported. This is possible energetically because one of the two molecules moves in accordance with a concentration gradient, and this energetically favorable event is permitted only upon concomitant movement of a desired compound against the prevailing concentration gradient. Single molecules may be transported across the membrane against the concentration gradient in an energy-driven process, such as that utilized by the ABC transporters. In this system, the transport protein located in the membrane has an ATP-binding cassette; upon binding of the target molecule, the ATP is converted to ADP+Pi, and the resulting release of energy is used to drive the movement of the target molecule to the opposite face of the membrane, facilitated by the transporter. For more detailed descriptions of all of these transport systems, see: Bamberg, E. et al., (1993) “Charge transport of ion pumps on lipid bilayer membranes”,  Q. Rev. Biophys.  26: 1-25; Findlay, J. B. C. (1991) “Structure and function in membrane transport systems”,  Curr. Opin. Struct. Biol.  1:804-810; Higgins, C. F. (1992) “ABC transporters from microorganisms to man”,  Ann. Rev. Cell Biol.  8: 67-113; Gennis, R. B. (1989) “Pores, Channels and Transporters”, in: Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 270-322; and Nikaido, H. and Saier, H. (1992) “Transport proteins in bacteria: common themes in their design”,  Science  258: 936-942, and references contained within each of these references.  
      The synthesis of membranes is a well-characterized process involving a number of components, the most important of which are lipid molecules. Lipid synthesis may be divided into two parts: the synthesis of fatty acids and their attachment to sn-glycerol-3-phosphate, and the addition or modification of a polar head group. Typical lipids utilized in bacterial membranes include phospholipids, glycolipids, sphingolipids, and phosphoglycerides. Fatty acid synthesis begins with the conversion of acetyl CoA either to malonyl CoA by acetyl CoA carboxylase, or to acetyl-ACP by acetyltransacylase. Following a condensation reaction, these two product molecules together form acetoacetyl-ACP, which is converted by a series of condensation, reduction and dehydration reactions to yield a saturated fatty acid molecule having a desired chain length. The production of unsaturated fatty acids from such molecules is catalyzed by specific desaturases either aerobically, with the help of molecular oxygen, or anaerobically (for reference on fatty acid synthesis, see F. C. Neidhardt et al. (1996)  E. coli  and Salmonella. ASM Press: Washington, D.C., p. 612-636 and references contained therein; Lengeler et al. (eds) (1999) Biology of Procaryotes. Thieme: Stuttgart, New York, and references contained therein; and Magnuson, K. et al., (1993)  Microbiological Reviews  57: 522-542, and references contained therein). The cyclopropane fatty acids (CFA) are synthesized by a specific CFA-synthase using SAM as a cosubstrate. Branched chain fatty acids are synthesized from branched chain amino acids that are deaminated to yield branched chain 2-oxo-acids (see Lengeler et al., eds. (1999) Biology of Procaryotes. Thieme: Stuttgart, New York, and references contained therein). Another essential step in lipid synthesis is the transfer of fatty acids onto the polar head groups by, for example, glycerol-phosphate-acyltransferases. The combination of various precursor molecules and biosynthetic enzymes results in the production of different fatty acid molecules, which has a profound effect on the composition of the membrane.  
      III. Elements and Methods of the Invention  
      The present invention is based, at least in part, on the discovery of novel molecules, referred to herein as MCT nucleic acid and protein molecules, which control the production of cellular membranes in  C. glutamicum  and govern the movement of molecules across such membranes. In one embodiment, the MCT molecules participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum,  or in the transport of molecules across these membranes. In a preferred embodiment, the activity of the MCT molecules of the present invention to regulate membrane component production and membrane transport has an impact on the production of a desired fine chemical by this organism. In a particularly preferred embodiment, the MCT molecules of the invention are modulated in activity, such that the  C. glutamicum  metabolic pathways which the MCT proteins of the invention regulate are modulated in yield, production, and/or efficiency of production and the transport of compounds through the membranes is altered in efficiency, which either directly or indirectly modulates the yield, production, and/or efficiency of production of a desired fine chemical by  C. glutamicum.    
      The language, “MCT protein” or “MCT polypeptide” includes proteins which participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes. Examples of MCT proteins include those encoded by the MCT genes set forth in Table 1 and Appendix A. The terms “MCT gene” or “MCT nucleic acid sequence” include nucleic acid sequences encoding an MCT protein, which consist of a coding region and also corresponding untranslated 5′ and 3′ sequence regions. Examples of MCT genes include those set forth in Table 1. The terms “production” or “productivity” are art-recognized and include the concentration of the fermentation product (for example, the desired fine chemical) formed within a given time and a given fermentation volume (e.g., kg product per hour per liter). The term “efficiency of production” includes the time required for a particular level of production to be achieved (for example, how long it takes for the cell to attain a particular rate of output of a fine chemical). The term “yield” or “product/carbon yield” is art-recognized and includes the efficiency of the conversion of the carbon source into the product (i.e., fine chemical). This is generally written as, for example, kg product per kg carbon source. By increasing the yield or production of the compound, the quantity of recovered molecules, or of useful recovered molecules of that compound in a given amount of culture over a given amount of time is increased. The terms “biosynthesis” or a “biosynthetic pathway” are art-recognized and include the synthesis of a compound, preferably an organic compound, by a cell from intermediate compounds in what may be a multistep and highly regulated process. The terms “degradation” or a “degradation pathway” are art-recognized and include the breakdown of a compound, preferably an organic compound, by a cell to degradation products (generally speaking, smaller or less complex molecules) in what may be a multistep and highly regulated process. The language “metabolism” is art-recognized and includes the totality of the biochemical reactions that take place in an organism. The metabolism of a particular compound, then, (e.g., the metabolism of an amino acid such as glycine) comprises the overall biosynthetic, modification, and degradation pathways in the cell related to this compound.  
      In another embodiment, the MCT molecules of the invention are capable of modulating the production of a desired molecule, such as a fine chemical, in a microorganism such as  C. glutamicum . There are a number of mechanisms by which the alteration of an MCT protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a  C. glutamicum  strain incorporating such an altered protein. Those MCT proteins involved in the export of fine chemical molecules from the cell may be increased in number or activity such that greater quantities of these compounds are secreted to the extracellular medium, from which they are more readily recovered. Similarly, those MCT proteins involved in the import of nutrients necessary for the biosynthesis of one or more fine chemicals (e.g., phosphate, sulfate, nitrogen compounds, etc.) may be increased in number or activity such that these precursor, cofactor, or intermediate compounds are increased in concentration within the cell. Further, fatty acids and lipids themselves are desirable fine chemicals; by optimizing the activity or increasing the number of one or more MCT proteins of the invention which participate in the biosynthesis of these compounds, or by impairing the activity of one or more MCT proteins which are involved in the degradation of these compounds, it may be possible to increase the yield, production, and/or efficiency of production of fatty acid and lipid molecules from  C. glutamicum.    
      The mutagenesis of one or more MCT genes of the invention may also result in MCT proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from  C. glutamicum . For example, MCT proteins of the invention involved in the export of waste products may be increased in number or activity such that the normal metabolic wastes of the cell (possibly increased in quantity due to the overproduction of the desired fine chemical) are efficiently exported before they are able to damage nucleotides and proteins within the cell (which would decrease the viability of the cell) or to interfere with fine chemical biosynthetic pathways (which would decrease the yield, production, or efficiency of production of the desired fine chemical). Further, the relatively large intracellular quantities of the desired fine chemical may in itself be toxic to the cell, so by increasing the activity or number of transporters able to export this compound from the cell, one may increase the viability of the cell in culture, in turn leading to a greater number of cells in the culture producing the desired fine chemical. The MCT proteins of the invention may also be manipulated such that the relative amounts of different lipid and fatty acid molecules are produced. This may have a profound effect on the lipid composition of the membrane of the cell. Since each type of lipid has different physical properties, an alteration in the lipid composition of a membrane may significantly alter membrane fluidity. Changes in membrane fluidity can impact the transport of molecules across the membrane, as well as the integrity of the cell, both of which have a profound effect on the production of fine chemicals from  C. glutamicum  in large-scale fermentative culture.  
      The isolated nucleic acid sequences of the invention are contained within the genome of a  Corynebacterium glutamicum  strain available through the American Type Culture Collection, given designation ATCC 13032. The nucleotide sequence of the isolated  C. glutamicum  MCT DNAs and the predicted amino acid sequences of the  C. glutamicum  MCT proteins are shown in Appendices A and B, respectively. Computational analyses were performed which classified and/or identified these nucleotide sequences as sequences which encode proteins involved in the metabolism of cellular membrane components or proteins involved in the transport of compounds across such membranes.  
      The present invention also pertains to proteins which have an amino acid sequence which is substantially homologous to an amino acid sequence of Appendix B. As used herein, a protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence is least about 50% homologous to the selected amino acid sequence, e.g., the entire selected amino acid sequence. A protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence can also be least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to the selected amino acid sequence.  
      The MCT protein or a biologically active portion or fragment thereof of the invention can participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes, or have one or more of the activities set forth in Table 1.  
      Various aspects of the invention are described in further detail in the following subsections:  
      A. Isolated Nucleic Acid Molecules  
      One aspect of the invention pertains to isolated nucleic acid molecules that encode MCT polypeptides or biologically active portions thereof, as well as nucleic acid fragments sufficient for use as hybridization probes or primers for the identification or amplification of MCT-encoding nucleic acid (e.g., MCT DNA). As used herein, the term “nucleic acid molecule” is intended to include DNA molecules (e.g., cDNA or genomic DNA) and RNA molecules (e.g., mRNA) and analogs of the DNA or RNA generated using nucleotide analogs. This term also encompasses untranslated sequence located at both the 3′ and 5′ ends of the coding region of the gene: at least about 100 nucleotides of sequence upstream from the 5′ end of the coding region and at least about 20 nucleotides of sequence downstream from the 3′ end of the coding region of the gene. The nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA. An “isolated” nucleic acid molecule is one which is separated from other nucleic acid molecules which are present in the natural source of the nucleic acid. Preferably, an “isolated” nucleic acid is free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5′ and 3′ ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated MCT nucleic acid molecule can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived (e.g, a  C. glutamicum  cell). Moreover, an “isolated” nucleic acid molecule, such as a DNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized.  
      A nucleic acid molecule of the present invention, e.g., a nucleic acid molecule having a nucleotide sequence of Appendix A, or a portion thereof, can be isolated using standard molecular biology techniques and the sequence information provided herein. For example, a  C. glutamicum  MCT DNA can be isolated from a  C. glutamicum  library using all or portion of one of the sequences of Appendix A as a hybridization probe and standard hybridization techniques (e.g., as described in Sambrook, J., Fritsh, E. F., and Maniatis, T.  Molecular Cloning: A Laboratory Manual.  2 nd, ed., Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989). Moreover, a nucleic acid molecule encompassing all or a portion of one of the sequences of Appendix A can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this sequence (e.g., a nucleic acid molecule encompassing all or a portion of one of the sequences of Appendix A can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this same sequence of Appendix A). For example, mRNA can be isolated from normal endothelial cells (e.g., by the guanidinium-thiocyanate extraction procedure of Chirgwin et al. (1979)  Biochemistry  18: 5294-5299) and DNA can be prepared using reverse transcriptase (e.g., Moloney MLV reverse transcriptase, available from Gibco/BRL, Bethesda, Md.; or AMV reverse transcriptase, available from Seikagaku America, Inc., St. Petersburg, Fla.). Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based upon one of the nucleotide sequences shown in Appendix A. A nucleic acid of the invention can be amplified using cDNA or, alternatively, genomic DNA, as a template and appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to an MCT nucleotide sequence can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.  
      In a preferred embodiment, an isolated nucleic acid molecule of the invention comprises one of the nucleotide sequences shown in Appendix A. The sequences of Appendix A correspond to the  Corynebacterium glutamicum  MCT DNAs of the invention. This DNA comprises sequences encoding MCT proteins (i.e., the “coding region”, indicated in each sequence in Appendix A), as well as 5′ untranslated sequences and 3′ untranslated sequences, also indicated in Appendix A. Alternatively, the nucleic acid molecule can comprise only the coding region of any of the sequences in Appendix A.  
      For the purposes of this application, it will be understood that each of the sequences set forth in Appendix A has an identifying RXA, RXN, RXS, or RXC number having the designation “RXA”, “RXN”, “RXS” or “RXC” followed by 5 digits (i.e., RXA02099, RXN03097, RXS00148, or RXC01748). Each of these sequences comprises up to three parts: a 5′ upstream region, a coding region, and a downstream region. Each of these three regions is identified by the same RXA, RXN, RXS, or RXC designation to eliminate confusion. The recitation “one of the sequences in Appendix A”, then, refers to any of the sequences in Appendix A, which may be distinguished by their differing RXA, RXN, RXS, or RXC designations. The coding region of each of these sequences is translated into a corresponding amino acid sequence, which is set forth in Appendix B. The sequences of Appendix B are identified by the same RXA, RXN, RXS, or RXC designations as Appendix A, such that they can be readily correlated. For example, the amino acid sequences in Appendix B designated RXA02099, RXN03097, RXS00148, and RXC01748 are translations of the coding region of the nucleotide sequences of nucleic acid molecules RXA02099, RXN03097, RXS00148, and RXC01748, respectively, in Appendix A. Each of the RXA, RXN, RXS, and RXC nucleotide and amino acid sequences of the invention has also been assigned a SEQ ID NO, as indicated in Table 1. For example, as set forth in Table 1, the nucleotide sequence of RXA00104 is SEQ ID NO:5, and the amino acid sequence of RXA00104 is SEQ ID NO:6.  
      Several of the genes of the invention are “F-designated genes”. An F-designated gene includes those genes set forth in Table 1 which have an ‘F’ in front of the RXA, RXN, RXS, or RXC designation. For example, SEQ ID NO:11, designated, as indicated on Table 1, as “F RXA02581”, is an F-designated gene, as are SEQ ID NOs: 31, 33, and 43 (designated on Table 1 as “F RXA02487”, “F RXA02490”, and “F RXA02809”, respectively).  
      In one embodiment, the nucleic acid molecules of the present invention are not intended to include those compiled in Table 2. In the case of the dapD gene, a sequence for this gene was published in Wehrmann, A., et al. (1998)  J. Bacteriol.  180(12): 3159-3165. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.  
      In another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule which is a complement of one of the nucleotide sequences shown in Appendix A, or a portion thereof. A nucleic acid molecule which is complementary to one of the nucleotide sequences shown in Appendix A is one which is sufficiently complementary to one of the nucleotide sequences shown in Appendix A such that it can hybridize to one of the nucleotide sequences shown in Appendix A, thereby forming a stable duplex.  
      In still another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence shown in Appendix A, or a portion thereof. Ranges and identity values intermediate to the above-recited ranges, (e.g., 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In an additional preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, to one of the nucleotide sequences shown in Appendix A, or a portion thereof.  
      Moreover, the nucleic acid molecule of the invention can comprise only a portion of the coding region of one of the sequences in Appendix A, for example a fragment which can be used as a probe or primer or a fragment encoding a biologically active portion of an MCT protein. The nucleotide sequences determined from the cloning of the MCT genes from  C. glutamicum  allows for the generation of probes and primers designed for use in identifying and/or cloning MCT homologues in other cell types and organisms, as well as MCT homologues from other  Corynebacteria  or related species. The probe/primer typically comprises substantially purified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, preferably about 25, more preferably about 40, 50 or 75 consecutive nucleotides of a sense strand of one of the sequences set forth in Appendix A, an anti-sense sequence of one of the sequences set forth in Appendix A, or naturally occurring mutants thereof. Primers based on a nucleotide sequence of Appendix A can be used in PCR reactions to clone MCT homologues. Probes based on the MCT nucleotide sequences can be used to detect transcripts or genomic sequences encoding the same or homologous proteins. In preferred embodiments, the probe further comprises a label group attached thereto, e.g. the label group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme co-factor. Such probes can be used as a part of a diagnostic test kit for identifying cells which misexpress an MCT protein, such as by measuring a level of an MCT-encoding nucleic acid in a sample of cells, e.g., detecting MCT mRNA levels or determining whether a genomic MCT gene has been mutated or deleted.  
      In one embodiment, the nucleic acid molecule of the invention encodes a protein or portion thereof which includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of Appendix B such that the protein or portion thereof maintains the ability to participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes. As used herein, the language “sufficiently homologous” refers to proteins or portions thereof which have amino acid sequences which include a minimum number of identical or equivalent (e.g., an amino acid residue which has a similar side chain as an amino acid residue in one of the sequences of Appendix B) amino acid residues to an amino acid sequence of Appendix B such that the protein or portion thereof is able to participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes. Protein members of such membrane component metabolic pathways or membrane transport systems, as described herein, may play a role in the production and secretion of one or more fine chemicals. Examples of such activities are also described herein. Thus, “the function of an MCT protein” contributes either directly or indirectly to the yield, production, and/or efficiency of production of one or more fine chemicals. Examples of MCT protein activities are set forth in Table 1.  
      In another embodiment, the protein is at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of Appendix B.  
      Portions of proteins encoded by the MCT nucleic acid molecules of the invention are preferably biologically active portions of one of the MCT proteins. As used herein, the term “biologically active portion of an MCT protein” is intended to include a portion, e.g., a domain/motif, of an MCT protein that participates in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes, or has an activity as set forth in Table 1. To determine whether an MCT protein or a biologically active portion thereof can participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes, an assay of enzymatic activity may be performed. Such assay methods are well known to those of ordinary skill in the art, as detailed in Example 8 of the Exemplification.  
      Additional nucleic acid fragments encoding biologically active portions of an MCT protein can be prepared by isolating a portion of one of the sequences in Appendix B, expressing the encoded portion of the MCT protein or peptide (e.g., by recombinant expression in vitro) and assessing the activity of the encoded portion of the MCT protein or peptide.  
      The invention further encompasses nucleic acid molecules that differ from one of the nucleotide sequences shown in Appendix A (and portions thereof) due to degeneracy of the genetic code and thus encode the same MCT protein as that encoded by the nucleotide sequences shown in Appendix A. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown in Appendix B. In a still further embodiment, the nucleic acid molecule of the invention encodes a full length  C. glutamicum  protein which is substantially homologous to an amino acid sequence of Appendix B (encoded by an open reading frame shown in Appendix A).  
      It will be understood by one of ordinary skill in the art that in one embodiment the sequences of the invention are not meant to include the sequences of the prior art, such as those Genbank sequences set forth in Tables 2 or 4 which were available prior to the present invention. In one embodiment, the invention includes nucleotide and amino acid sequences having a percent identity to a nucleotide or amino acid sequence of the invention which is greater than that of a sequence of the prior art (e.g., a Genbank sequence (or the protein encoded by such a sequence) set forth in Tables 2 or 4). For example, the invention includes a nucleotide sequence which is greater than and/or at least 38% identical to the nucleotide sequence designated RXA01420 (SEQ ID NO:7), a nucleotide sequence which is greater than and/or at least 43% identical to the nucleotide sequence designated RXA00104 (SEQ ID NO:5), and a nucleotide sequence which is greater than and/or at least 45% identical to the nucleotide sequence designated RXA02173 (SEQ ID NO:25). One of ordinary skill in the art would be able to calculate the lower threshold of percent identity for any given sequence of the invention by examining the GAP-calculated percent identity scores set forth in Table 4 for each of the three top hits for the given sequence, and by subtracting the highest GAP-calculated percent identity from 100 percent. One of ordinary skill in the art will also appreciate that nucleic acid and amino acid sequences having percent identities greater than the lower threshold so calculated (e.g., at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more identical) are also encompassed by the invention.  
      In addition to the  C. glutamicum  MCT nucleotide sequences shown in Appendix A, it will be appreciated by one of ordinary skill in the art that DNA sequence polymorphisms that lead to changes in the amino acid sequences of MCT proteins may exist within a population (e.g., the  C. glutamicum  population). Such genetic polymorphism in the MCT gene may exist among individuals within a population due to natural variation. As used herein, the terms “gene” and “recombinant gene” refer to nucleic acid molecules comprising an open reading frame encoding an MCT protein, preferably a  C. glutamicum  MCT protein. Such natural variations can typically result in 1-5% variance in the nucleotide sequence of the MCT gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in MCT that are the result of natural variation and that do not alter the functional activity of MCT proteins are intended to be within the scope of the invention.  
      Nucleic acid molecules corresponding to natural variants and non- C. glutamicum  homologues of the  C. glutamicum  MCT DNA of the invention can be isolated based on their homology to the  C. glutamicum  MCT nucleic acid disclosed herein using the  C. glutamicum  DNA, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions. Accordingly, in another embodiment, an isolated nucleic acid molecule of the invention is at least 15 nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising a nucleotide sequence of Appendix A. In other embodiments, the nucleic acid is at least 30, 50, 100, 250 or more nucleotides in length. As used herein, the term “hybridizes under stringent conditions” is intended to describe conditions for hybridization and washing under which nucleotide sequences at least 60% homologous to each other typically remain hybridized to each other. Preferably, the conditions are such that sequences at least about 65%, more preferably at least about 70%, and even more preferably at least about 75% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those of ordinary skill in the art and can be found in  Current Protocols in Molecular Biology , John Wiley &amp; Sons, N.Y. (1989), 6.3.1-6.3.6. A preferred, non-limiting example of stringent hybridization conditions are hybridization in 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50-65° C. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a sequence of Appendix A corresponds to a naturally-occurring nucleic acid molecule. As used herein, a “naturally-occurring” nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (e.g., encodes a natural protein). In one embodiment, the nucleic acid encodes a natural  C. glutamicum  MCT protein.  
      In addition to naturally-occurring variants of the MCT sequence that may exist in the population, one of ordinary skill in the art will further appreciate that changes can be introduced by mutation into a nucleotide sequence of Appendix A, thereby leading to changes in the amino acid sequence of the encoded MCT protein, without altering the functional ability of the MCT protein. For example, nucleotide substitutions leading to amino acid substitutions at “non-essential” amino acid residues can be made in a sequence of Appendix A. A “non-essential” amino acid residue is a residue that can be altered from the wild-type sequence of one of the MCT proteins (Appendix B) without altering the activity of said MCT protein, whereas an “essential” amino acid residue is required for MCT protein activity. Other amino acid residues, however, (e.g., those that are not conserved or only semi-conserved in the domain having MCT activity) may not be essential for activity and thus are likely to be amenable to alteration without altering MCT activity.  
      Accordingly, another aspect of the invention pertains to nucleic acid molecules encoding MCT proteins that contain changes in amino acid residues that are not essential for MCT activity. Such MCT proteins differ in amino acid sequence from a sequence contained in Appendix B yet retain at least one of the MCT activities described herein. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 50% homologous to an amino acid sequence of Appendix B and is capable of participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes, or has one or more activities set forth in Table 1. Preferably, the protein encoded by the nucleic acid molecule is at least about 50-60% homologous to one of the sequences in Appendix B, more preferably at least about 60-70% homologous to one of the sequences in Appendix B, even more preferably at least about 70-80%, 80-90%, 90-95% homologous to one of the sequences in Appendix B, and most preferably at least about 96%, 97%, 98%, or 99% homologous to one of the sequences in Appendix B.  
      To determine the percent homology of two amino acid sequences (e.g., one of the sequences of Appendix B and a mutant form thereof) or of two nucleic acids, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of one protein or nucleic acid for optimal alignment with the other protein or nucleic acid). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in one sequence (e.g., one of the sequences of Appendix B) is occupied by the same amino acid residue or nucleotide as the corresponding position in the other sequence (e.g., a mutant form of the sequence selected from Appendix B), then the molecules are homologous at that position (i.e., as used herein amino acid or nucleic acid “homology” is equivalent to amino acid or nucleic acid “identity”). The percent homology between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % homology=# of identical positions/total# of positions×100).  
      An isolated nucleic acid molecule encoding an MCT protein homologous to a protein sequence of Appendix B can be created by introducing one or more nucleotide substitutions, additions or deletions into a nucleotide sequence of Appendix A such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein. Mutations can be introduced into one of the sequences of Appendix A by standard techniques, such as site-directed mutagenesis and PCR-mediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A “conservative amino acid substitution” is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in an MCT protein is preferably replaced with another amino acid residue from the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly along all or part of an MCT coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for an MCT activity described herein to identify mutants that retain MCT activity. Following mutagenesis of one of the sequences of Appendix A, the encoded protein can be expressed recombinantly and the activity of the protein can be determined using, for example, assays described herein (see Example 8 of the Exemplification).  
      In addition to the nucleic acid molecules encoding MCT proteins described above, another aspect of the invention pertains to isolated nucleic acid molecules which are antisense thereto. An “antisense” nucleic acid comprises a nucleotide sequence which is complementary to a “sense” nucleic acid encoding a protein, e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mRNA sequence. Accordingly, an antisense nucleic acid can hydrogen bond to a sense nucleic acid. The antisense nucleic acid can be complementary to an entire MCT coding strand, or to only a portion thereof. In one embodiment, an antisense nucleic acid molecule is antisense to a “coding region” of the coding strand of a nucleotide sequence encoding an MCT protein. The term “coding region” refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues (e.g., the entire coding region of SEQ ID NO:5 (RXA00104 in Appendix A) comprises nucleotides 1 to 756). In another embodiment, the antisense nucleic acid molecule is antisense to a “noncoding region” of the coding strand of a nucleotide sequence encoding MCT. The term “noncoding region” refers to 5′ and 3′ sequences which flank the coding region that are not translated into amino acids (i.e., also referred to as 5′ and 3′ untranslated regions).  
      Given the coding strand sequences encoding MCT disclosed herein (e.g., the sequences set forth in Appendix A), antisense nucleic acids of the invention can be designed according to the rules of Watson and Crick base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of MCT mRNA, but more preferably is an oligonucleotide which is antisense to only a portion of the coding or noncoding region of MCT mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of MCT mRNA. An antisense oligonucleotide can be, for example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis and enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid (e.g., an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, e.g., phosphorothioate derivatives and acridine substituted nucleotides can be used. Examples of modified nucleotides which can be used to generate the antisense nucleic acid include 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl-2-thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5′-methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v), 5-methyl-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation (i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).  
      The antisense nucleic acid molecules of the invention are typically administered to a cell or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding an MCT protein to thereby inhibit expression of the protein, e.g., by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule which binds to DNA duplexes, through specific interactions in the major groove of the double helix. The antisense molecule can be modified such that it specifically binds to a receptor or an antigen expressed on a selected cell surface, e.g., by linking the antisense nucleic acid molecule to a peptide or an antibody which binds to a cell surface receptor or antigen. The antisense nucleic acid molecule can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of the antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong prokaryotic, viral, or eukaryotic promoter are preferred.  
      In yet another embodiment, the antisense nucleic acid molecule of the invention is an α-anomeric nucleic acid molecule. An α-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual β-units, the strands run parallel to each other (Gaultier et al. (1987)  Nucleic Acids. Res.  15:6625-6641). The antisense nucleic acid molecule can also comprise a 2′-o-methylribonucleotide (Inoue et al. (1987)  Nucleic Acids Res.  15:6131-6148) or a chimeric RNA-DNA analogue (Inoue et al. (1987)  FEBS Lett.  215:327-330).  
      In still another embodiment, an antisense nucleic acid of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes (e.g., hammerhead ribozymes (described in Haselhoff and Gerlach (1988)  Nature  334:585-591)) can be used to catalytically cleave MCT mRNA transcripts to thereby inhibit translation of MCT mRNA. A ribozyme having specificity for an MCT-encoding nucleic acid can be designed based upon the nucleotide sequence of an MCT DNA disclosed herein (i.e., SEQ ID NO. 5 (RXA00104) in Appendix A)). For example, a derivative of a  Tetrahymena L- 19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in an MCT-encoding mRNA. See, e.g., Cech et al. U.S. Pat. No. 4,987,071 and Cech et al. U.S. Pat. No. 5,116,742. Alternatively, MCT mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, e.g., Bartel, D. and Szostak, J. W. (1993)  Science  261:1411-1418.  
      Alternatively, MCT gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of an MCT nucleotide sequence (e.g., an MCT promoter and/or enhancers) to form triple helical structures that prevent transcription of an MCT gene in target cells. See generally, Helene, C. (1991)  Anticancer Drug Des.  6(6):569-84; Helene, C. et al. (1992)  Ann. N.Y. Acad. Sci.  660:27-36; and Maher, L. J. (1992)  Bioassays  14(12):807-15.  
      B. Recombinant Expression Vectors and Host Cells  
      Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding an MCT protein (or a portion thereof). As used herein, the term “vector” refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a “plasmid”, which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as “expression vectors”. In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, “plasmid” and “vector” can be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.  
      The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, “operably linked” is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence (e.g., in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell). The term “regulatory sequence” is intended to include promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel;  Gene Expression Technology: Methods in Enzymology  185, Academic Press, San Diego, Calif. (1990). Regulatory sequences include those which direct constitutive expression of a nucleotide sequence in many types of host cell and those which direct expression of the nucleotide sequence only in certain host cells. Preferred regulatory sequences are, for example, promoters such as cos-, tac-, trp-, tet-, trp-tet-, lpp-, lac-, lpp-lac-, lacI q -, T7-, T5-, T3-, gal-, trc-, ara-, SP6-, amy, SPO2, λ-P R -or λ P L , which are used preferably in bacteria. Additional regulatory sequences are, for example, promoters from yeasts and fungi, such as ADC1, MFα, AC, P-60, CYC1, GAPDH, TEF, rp28, ADH, promoters from plants such as CaMV/35S, SSU, OCS, lib4, usp, STLS1, B33, nos or ubiquitin- or phaseolin-promoters. It is also possible to use artificial promoters. It will be appreciated by one of ordinary skill in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein (e.g., MCT proteins, mutant forms of MCT proteins, fusion proteins, etc.).  
      The recombinant expression vectors of the invention can be designed for expression of MCT proteins in prokaryotic or eukaryotic cells. For example, MCT genes can be expressed in bacterial cells such as  C. glutamicum , insect cells (using baculovirus expression vectors), yeast and other fungal cells (see Romanos, M. A. et al. (1992) “Foreign gene expression in yeast: a review”,  Yeast  8: 423-488; van den Hondel, C.A.M.J.J. et al. (1991) “Heterologous gene expression in filamentous fungi” in: More Gene Manipulations in Fungi, J. W. Bennet &amp; L. L. Lasure, eds., p. 396-428: Academic Press: San Diego; and van den Hondel, C.A.M.J.J. &amp; Punt, P. J. (1991) “Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, J. F. et al., eds., p. 1-28, Cambridge University Press: Cambridge), algae and multicellular plant cells (see Schmidt, R. and Willmitzer, L. (1988) High efficiency  Agrobacterium tumefaciens —mediated transformation of  Arabidopsis thaliana  leaf and cotyledon explants”  Plant Cell Rep.:  583-586), or mammalian cells. Suitable host cells are discussed further in Goeddel,  Gene Expression Technology: Methods in Enzymology  185, Academic Press, San Diego, Calif. (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.  
      Expression of proteins in prokaryotes is most often carried out with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein but also to the C-terminus or fused within suitable regions in the proteins. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant protein; 2) to increase the solubility of the recombinant protein; and 3) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase.  
      Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith, D. B. and Johnson, K. S. (1988)  Gene  67:31-40), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. In one embodiment, the coding sequence of the MCT protein is cloned into a pGEX expression vector to create a vector encoding a fusion protein comprising, from the N-terminus to the C-terminus, GST-thrombin cleavage site-X protein. The fusion protein can be purified by affinity chromatography using glutathione-agarose resin. Recombinant MCT protein unfused to GST can be recovered by cleavage of the fusion protein with thrombin.  
      Examples of suitable inducible non-fusion  E. coli  expression vectors include pTrc (Amann et al., (1988)  Gene  69:301-315) pLG338, pACYC184, pBR322, pUC18, pUC19, pKC30, pRep4, pHS1, pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN-III113-B1, λgt11, pBdC1, and pET 11d (Studier et al,  Gene Expression Technology: Methods in Enzymology  185, Academic Press, San Diego, Calif. (1990) 60-89 ; and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018). Target gene expression from the pTrc vector relies on host RNA polymerase transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pET 11d vector relies on transcription from a T7 gn10-lac fusion promoter mediated by a coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21(DE3) or HMS174(DE3) from a resident λ prophage harboring a T7 gn1 gene under the transcriptional control of the lacUV 5 promoter. For transformation of other varieties of bacteria, appropriate vectors may be selected. For example, the plasmids pIJ101, pIJ364, pIJ702 and pIJ361 are known to be useful in transforming Streptomyces, while plasmids pUB110, pC194, or pBD214 are suited for transformation of Bacillus species. Several plasmids of use in the transfer of genetic information into Corynebacterium include pHM1519, pBL1, pSA77, or pAJ667 (Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: N.Y. IBSN 0 444 904018).  
      One strategy to maximize recombinant protein expression is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, S.,  Gene Expression Technology: Methods in Enzymology  185, Academic Press, San Diego, Calif. (1990) 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in the bacterium chosen for expression, such as  C. glutamicum  (Wada et al. (1992)  Nucleic Acids Res.  20:2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.  
      In another embodiment, the MCT protein expression vector is a yeast expression vector. Examples of vectors for expression in yeast  S. cerevisiae  include pYepSec1 (Baldari, et al., (1987)  Embo J.  6:229-234), 2μ, pAG-1, Yep6, Yep13, pEMBLYe23, pMFa (Kurjan and Herskowitz, (1982)  Cell  30:933-943), pJRY88 (Schultz et al., (1987)  Gene  54:113-123), and pYES2 (Invitrogen Corporation, San Diego, Calif.). Vectors and methods for the construction of vectors appropriate for use in other fungi, such as the filamentous fungi, include those detailed in: van den Hondel, C.A.M.J.J. &amp; Punt, P. J. (1991) “Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, J. F. Peberdy, et al., eds., p. 1-28, Cambridge University Press: Cambridge, and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: N.Y. (IBSN 0 444 904018).  
      Alternatively, the MCT proteins of the invention can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., Sf9 cells) include the pAc series (Smith et al. (1983)  Mol. Cell Biol.  3:2156-2165) and the pVL series (Lucklow and Summers (1989)  Virology  170:31-39).  
      In another embodiment, the MCT proteins of the invention may be expressed in unicellular plant cells (such as algae) or in plant cells from higher plants (e.g., the spermatophytes, such as crop plants). Examples of plant expression vectors include those detailed in: Becker, D., Kemper, E., Schell, J. and Masterson, R. (1992) “New plant binary vectors with selectable markers located proximal to the left border”,  Plant Mol. Biol.  20: 1195-1197; and Bevan, M. W. (1984) “Binary  Agrobacterium  vectors for plant transformation”,  Nuc. Acid Res.  12: 8711-8721, and include pLGV23, pGHlac+, pBIN19, pAK2004, and pDH51 (Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: N.Y. IBSN 0 444 904018).  
      In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, B. (1987)  Nature  329:840) and pMT2PC (Kaufman et al. (1987)  EMBO J.  6:187-195). When used in mammalian cells, the expression vector&#39;s control functions are often provided by viral regulatory elements. For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see chapters 16 and 17 of Sambrook, J., Fritsh, E. F., and Maniatis, T.  Molecular Cloning: A Laboratory Manual.  2 nd, ed., Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989.  
      In another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type (e.g., tissue-specific regulatory elements are used to express the nucleic acid). Tissue-specific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific; Pinkert et al. (1987)  Genes Dev.  1:268-277), lymphoid-specific promoters (Calame and Eaton (1988)  Adv. Immunol.  43:235-275), in particular promoters of T cell receptors (Winoto and Baltimore (1989)  EMBO J.  8:729-733) and immunoglobulins (Banerji et al. (1983)  Cell  33:729-740; Queen and Baltimore (1983)  Cell  33:741-748), neuron-specific promoters (e.g., the neurofilament promoter; Byrne and Ruddle (1989)  PNAS  86:5473-5477), pancreas-specific promoters (Edlund et al. (1985)  Science  230:912-916), and mammary gland-specific promoters (e.g., milk whey promoter; U.S. Pat. No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, for example the murine hox promoters (Kessel and Gruss (1990)  Science  249:374-379) and the α-fetoprotein promoter (Campes and Tilghman (1989)  Genes Dev.  3:537-546).  
      The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operatively linked to a regulatory sequence in a manner which allows for expression (by transcription of the DNA molecule) of an RNA molecule which is antisense to MCT mRNA. Regulatory sequences operatively linked to a nucleic acid cloned in the antisense orientation can be chosen which direct the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen which direct constitutive, tissue specific or cell type specific expression of antisense RNA. The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity of which can be determined by the cell type into which the vector is introduced. For a discussion of the regulation of gene expression using antisense genes see Weintraub, H. et al., Antisense RNA as a molecular tool for genetic analysis,  Reviews—Trends in Genetics, Vol.  1(1) 1986.  
      Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms “host cell” and “recombinant host cell” are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.  
      A host cell can be any prokaryotic or eukaryotic cell. For example, an MCT protein can be expressed in bacterial cells such as  C. glutamicum , insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to one of ordinary skill in the art. Microorganisms related to  Corynebacterium glutamicum  which may be conveniently used as host cells for the nucleic acid and protein molecules of the invention are set forth in Table 3.  
      Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms “transformation” and “transfection”, “conjugation” and “transduction” are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid (e.g., linear DNA or RNA (e.g., a linearized vector or a gene construct alone without a vector) or nucleic acid in the form of a vector (e.g., a plasmid, phage, phasmid, phagemid, transposon or other DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, natural competence, chemical-mediated transfer, or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. ( Molecular Cloning: A Laboratory Manual.  2 nd, ed., Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989), and other laboratory manuals.  
      For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene that encodes a selectable marker (e.g., resistance to antibiotics) is generally introduced into the host cells along with the gene of interest. Preferred selectable markers include those which confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding an MCT protein or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by, for example, drug selection (e.g., cells that have incorporated the selectable marker gene will survive, while the other cells die).  
      To create a homologous recombinant microorganism, a vector is prepared which contains at least a portion of an MCT gene into which a deletion, addition or substitution has been introduced to thereby alter, e.g., functionally disrupt, the MCT gene. Preferably, this MCT gene is a  Corynebacterium glutamicum  MCT gene, but it can be a homologue from a related bacterium or even from a mammalian, yeast, or insect source. In a preferred embodiment, the vector is designed such that, upon homologous recombination, the endogenous MCT gene is functionally disrupted (i.e., no longer encodes a functional protein; also referred to as a “knock out” vector). Alternatively, the vector can be designed such that, upon homologous recombination, the endogenous MCT gene is mutated or otherwise altered but still encodes functional protein (e.g., the upstream regulatory region can be altered to thereby alter the expression of the endogenous MCT protein). In the homologous recombination vector, the altered portion of the MCT gene is flanked at its 5′ and 3′ ends by additional nucleic acid of the MCT gene to allow for homologous recombination to occur between the exogenous MCT gene carried by the vector and an endogenous MCT gene in a microorganism. The additional flanking MCT nucleic acid is of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5′ and 3′ ends) are included in the vector (see e.g., Thomas, K. R., and Capecchi, M. R. (1987) Cell 51: 503 for a description of homologous recombination vectors). The vector is introduced into a microorganism (e.g., by electroporation) and cells in which the introduced MCT gene has homologously recombined with the endogenous MCT gene are selected, using art-known techniques.  
      In another embodiment, recombinant microorganisms can be produced which contain selected systems which allow for regulated expression of the introduced gene. For example, inclusion of an MCT gene on a vector placing it under control of the lac operon permits expression of the MCT gene only in the presence of IPTG. Such regulatory systems are well known in the art.  
      In another embodiment, an endogenous MCT gene in a host cell is disrupted (e.g., by homologous recombination or other genetic means known in the art) such that expression of its protein product does not occur. In another embodiment, an endogenous or introduced MCT gene in a host cell has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional MCT protein. In still another embodiment, one or more of the regulatory regions (e.g., a promoter, repressor, or inducer) of an MCT gene in a microorganism has been altered (e.g., by deletion, truncation, inversion, or point mutation) such that the expression of the MCT gene is modulated. One of ordinary skill in the art will appreciate that host cells containing more than one of the described MCT gene and protein modifications may be readily produced using the methods of the invention, and are meant to be included in the present invention.  
      A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce (i.e., express) an MCT protein. Accordingly, the invention further provides methods for producing MCT proteins using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding an MCT protein has been introduced, or into which genome has been introduced a gene encoding a wild-type or altered MCT protein) in a suitable medium until MCT protein is produced. In another embodiment, the method further comprises isolating MCT proteins from the medium or the host cell.  
      C. Isolated MCT Proteins  
      Another aspect of the invention pertains to isolated MCT proteins, and biologically active portions thereof. An “isolated” or “purified” protein or biologically active portion thereof is substantially free of cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. The language “substantially free of cellular material” includes preparations of MCT protein in which the protein is separated from cellular components of the cells in which it is naturally or recombinantly produced. In one embodiment, the language “substantially free of cellular material” includes preparations of MCT protein having less than about 30% (by dry weight) of non-MCT protein (also referred to herein as a “contaminating protein”), more preferably less than about 20% of non-MCT protein, still more preferably less than about 10% of non-MCT protein, and most preferably less than about 5% non-MCT protein. When the MCT protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, i.e., culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the protein preparation. The language “substantially free of chemical precursors or other chemicals” includes preparations of MCT protein in which the protein is separated from chemical precursors or other chemicals which are involved in the synthesis of the protein. In one embodiment, the language “substantially free of chemical precursors or other chemicals” includes preparations of MCT protein having less than about 30% (by dry weight) of chemical precursors or non-MCT chemicals, more preferably less than about 20% chemical precursors or non-MCT chemicals, still more preferably less than about 10% chemical precursors or non-MCT chemicals, and most preferably less than about 5% chemical precursors or non-MCT chemicals. In preferred embodiments, isolated proteins or biologically active portions thereof lack contaminating proteins from the same organism from which the MCT protein is derived. Typically, such proteins are produced by recombinant expression of, for example, a  C. glutamicum  MCT protein in a microorganism such as  C. glutamicum.    
      An isolated MCT protein or a portion thereof of the invention can participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes, or has one or more of the activities set forth in Table 1. In preferred embodiments, the protein or portion thereof comprises an amino acid sequence which is sufficiently homologous to an amino acid sequence of Appendix B such that the protein or portion thereof maintains the ability participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes. The portion of the protein is preferably a biologically active portion as described herein. In another preferred embodiment, an MCT protein of the invention has an amino acid sequence shown in Appendix B. In yet another preferred embodiment, the MCT protein has an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, to a nucleotide sequence of Appendix A. In still another preferred embodiment, the MCT protein has an amino acid sequence which is encoded by a nucleotide sequence that is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to one of the nucleic acid sequences of Appendix A, or a portion thereof. Ranges and identity values intermediate to the above-recited values, (e.g., 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. The preferred MCT proteins of the present invention also preferably possess at least one of the MCT activities described herein. For example, a preferred MCT protein of the present invention includes an amino acid sequence encoded by a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, to a nucleotide sequence of Appendix A, and which can participate in the metabolism of compounds necessary for the construction of cellular membranes in  C. glutamicum , or in the transport of molecules across these membranes, or which has one or more of the activities set forth in Table 1.  
      In other embodiments, the MCT protein is substantially homologous to an amino acid sequence of Appendix B and retains the functional activity of the protein of one of the sequences of Appendix B yet differs in amino acid sequence due to natural variation or mutagenesis, as described in detail in subsection I above. Accordingly, in another embodiment, the MCT protein is a protein which comprises an amino acid sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of Appendix B and which has at least one of the MCT activities described herein. Ranges and identity values intermediate to the above-recited values, (e.g., 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In another embodiment, the invention pertains to a full length  C. glutamicum  protein which is substantially homologous to an entire amino acid sequence of Appendix B.  
      Biologically active portions of an MCT protein include peptides comprising amino acid sequences derived from the amino acid sequence of an MCT protein, e.g., the an amino acid sequence shown in Appendix B or the amino acid sequence of a protein homologous to an MCT protein, which include fewer amino acids than a full length MCT protein or the full length protein which is homologous to an MCT protein, and exhibit at least one activity of an MCT protein. Typically, biologically active portions (peptides, e.g., peptides which are, for example, 5, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more amino acids in length) comprise a domain or motif with at least one activity of an MCT protein. Moreover, other biologically active portions, in which other regions of the protein are deleted, can be prepared by recombinant techniques and evaluated for one or more of the activities described herein. Preferably, the biologically active portions of an MCT protein include one or more selected domains/motifs or portions thereof having biological activity.  
      MCT proteins are preferably produced by recombinant DNA techniques. For example, a nucleic acid molecule encoding the protein is cloned into an expression vector (as described above), the expression vector is introduced into a host cell (as described above) and the MCT protein is expressed in the host cell. The MCT protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Alternative to recombinant expression, an MCT protein, polypeptide, or peptide can be synthesized chemically using standard peptide synthesis techniques. Moreover, native MCT protein can be isolated from cells (e.g., endothelial cells), for example using an anti-MCT antibody, which can be produced by standard techniques utilizing an MCT protein or fragment thereof of this invention.  
      The invention also provides MCT chimeric or fusion proteins. As used herein, an MCT “chimeric protein” or “fusion protein” comprises an MCT polypeptide operatively linked to a non-MCT polypeptide. An “MCT polypeptide” refers to a polypeptide having an amino acid sequence corresponding to an MCT protein, whereas a “non-MCT polypeptide” refers to a polypeptide having an amino acid sequence corresponding to a protein which is not substantially homologous to the MCT protein, e.g., a protein which is different from the MCT protein and which is derived from the same or a different organism. Within the fusion protein, the term “operatively linked” is intended to indicate that the MCT polypeptide and the non-MCT polypeptide are fused in-frame to each other. The non-MCT polypeptide can be fused to the N-terminus or C-terminus of the MCT polypeptide. For example, in one embodiment the fusion protein is a GST-MCT fusion protein in which the MCT sequences are fused to the C-terminus of the GST sequences. Such fusion proteins can facilitate the purification of recombinant MCT proteins. In another embodiment, the fusion protein is an MCT protein containing a heterologous signal sequence at its N-terminus. In certain host cells (e.g., mammalian host cells), expression and/or secretion of an MCT protein can be increased through use of a heterologous signal sequence.  
      Preferably, an MCT chimeric or fusion protein of the invention is produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, for example by employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, for example,  Current Protocols in Molecular Biology , eds. Ausubel et al. John Wiley &amp; Sons: 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety (e.g., a GST polypeptide). An MCT-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the MCT protein.  
      Homologues of the MCT protein can be generated by mutagenesis, e.g., discrete point mutation or truncation of the MCT protein. As used herein, the term “homologue” refers to a variant form of the MCT protein which acts as an agonist or antagonist of the activity of the MCT protein. An agonist of the MCT protein can retain substantially the same, or a subset, of the biological activities of the MCT protein. An antagonist of the MCT protein can inhibit one or more of the activities of the naturally occurring form of the MCT protein, by, for example, competitively binding to a downstream or upstream member of the cell membrane component metabolic cascade which includes the MCT protein, or by binding to an MCT protein which mediates transport of compounds across such membranes, thereby preventing translocation from taking place.  
      In an alternative embodiment, homologues of the MCT protein can be identified by screening combinatorial libraries of mutants, e.g., truncation mutants, of the MCT protein for MCT protein agonist or antagonist activity. In one embodiment, a variegated library of MCT variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of MCT variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential MCT sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins (e.g., for phage display) containing the set of MCT sequences therein. There are a variety of methods which can be used to produce libraries of potential MCT homologues from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential MCT sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (see, e.g., Narang, S. A. (1983)  Tetrahedron  39:3; Itakura et al. (1984)  Annu. Rev. Biochem.  53:323; Itakura et al. (1984)  Science  198:1056; Ike et al. (1983)  Nucleic Acid Res.  11:477.  
      In addition, libraries of fragments of the MCT protein coding can be used to generate a variegated population of MCT fragments for screening and subsequent selection of homologues of an MCT protein. In one embodiment, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of an MCT coding sequence with a nuclease under conditions wherein nicking occurs only about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double stranded DNA which can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by treatment with S1 nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes N-terminal, C-terminal and internal fragments of various sizes of the MCT protein.  
      Several techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of MCT homologues. The most widely used techniques, which are amenable to high through-put analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was detected. Recursive ensemble mutagenesis (REM), a new technique which enhances the frequency of functional mutants in the libraries, can be used in combination with the screening assays to identify MCT homologues (Arkin and Yourvan (1992)  PNAS  89:7811-7815; Delgrave et al. (1993)  Protein Engineering  6(3):327-331).  
      In another embodiment, cell based assays can be exploited to analyze a variegated MCT library, using methods well known in the art.  
      D. Uses and Methods of the Invention  
      The nucleic acid molecules, proteins, protein homologues, fusion proteins, primers, vectors, and host cells described herein can be used in one or more of the following methods: identification of  C. glutamicum  and related organisms; mapping of genomes of organisms related to  C. glutamicum  ; identification and localization of  C. glutamicum  sequences of interest; evolutionary studies; determination of MCT protein regions required for function; modulation of an MCT protein activity; modulation of the metabolism of one or more cell membrane components; modulation of the transmembrane transport of one or more compounds; and modulation of cellular production of a desired compound, such as a fine chemical.  
      The MCT nucleic acid molecules of the invention have a variety of uses. First, they may be used to identify an organism as being  Corynebacterium glutamicum  or a close relative thereof. Also, they may be used to identify the presence of  C. glutamicum  or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of  C. glutamicum  genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a  C. glutamicum  gene which is unique to this organism, one can ascertain whether this organism is present.  
      Although  Corynebacterium glutamicum  itself is nonpathogenic, it is related to pathogenic species, such as  Corynebacterium diphtheriae. Corynebacterium diphtheriae  is the causative agent of diphtheria, a rapidly developing, acute, febrile infection which involves both local and systemic pathology. In this disease, a local lesion develops in the upper respiratory tract and involves necrotic injury to epithelial cells; the bacilli secrete toxin which is disseminated through this lesion to distal susceptible tissues of the body. Degenerative changes brought about by the inhibition of protein synthesis in these tissues, which include heart, muscle, peripheral nerves, adrenals, kidneys, liver and spleen, result in the systemic pathology of the disease. Diphtheria continues to have high incidence in many parts of the world, including Africa, Asia, Eastern Europe and the independent states of the former Soviet Union. An ongoing epidemic of diphtheria in the latter two regions has resulted in at least 5,000 deaths since 1990. In one embodiment, the invention provides a method of identifying the presence or activity of  Cornyebacterium diphtheriae  in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention (e.g., the sequences set forth in Appendix A or Appendix B) in a subject, thereby detecting the presence or activity of  Corynebacterium diphtheriae  in the subject.  C. glutamicum  and  C. diphtheriae  are related bacteria, and many of the nucleic acid and protein molecules in  C. glutamicum  are homologous to  C. diphtheriae  nucleic acid and protein molecules, and can therefore be used to detect  C. diphtheriae  in a subject.  
      The nucleic acid and protein molecules of the invention may also serve as markers for specific regions of the genome. This has utility not only in the mapping of the genome, but also for functional studies of  C. glutamicum  proteins. For example, to identify the region of the genome to which a particular  C. glutamicum  DNA-binding protein binds, the  C. glutamicum  genome could be digested, and the fragments incubated with the DNA-binding protein. Those which bind the protein may be additionally probed with the nucleic acid molecules of the invention, preferably with readily detectable labels; binding of such a nucleic acid molecule to the genome fragment enables the localization of the fragment to the genome map of  C. glutamicum , and, when performed multiple times with different enzymes, facilitates a rapid determination of the nucleic acid sequence to which the protein binds. Further, the nucleic acid molecules of the invention may be sufficiently homologous to the sequences of related species such that these nucleic acid molecules may serve as markers for the construction of a genomic map in related bacteria, such as  Brevibacterium lactofermentum.    
      The MCT nucleic acid molecules of the invention are also useful for evolutionary and protein structural studies. The metabolic and transport processes in which the molecules of the invention participate are utilized by a wide variety of prokaryotic and eukaryotic cells; by comparing the sequences of the nucleic acid molecules of the present invention to those encoding similar enzymes from other organisms, the evolutionary relatedness of the organisms can be assessed. Similarly, such a comparison permits an assessment of which regions of the sequence are conserved and which are not, which may aid in determining those regions of the protein which are essential for the functioning of the enzyme. This type of determination is of value for protein engineering studies and may give an indication of what the protein can tolerate in terms of mutagenesis without losing function.  
      Manipulation of the MCT nucleic acid molecules of the invention may result in the production of MCT proteins having functional differences from the wild-type MCT proteins. These proteins may be improved in efficiency or activity, may be present in greater numbers in the cell than is usual, or may be decreased in efficiency or activity.  
      The invention provides methods for screening molecules which modulate the activity of an MCT protein, either by interacting with the protein itself or a substrate or binding partner of the MCT protein, or by modulating the transcription or translation of an MCT nucleic acid molecule of the invention. In such methods, a microorganism expressing one or more MCT proteins of the invention is contacted with one or more test compounds, and the effect of each test compound on the activity or level of expression of the MCT protein is assessed.  
      There are a number of mechanisms by which the alteration of an MCT protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a  C. glutamicum  strain incorporating such an altered protein. Recovery of fine chemical compounds from large-scale cultures of  C. glutamicum  is significantly improved if  C. glutamicum  secretes the desired compounds, since such compounds may be readily purified from the culture medium (as opposed to extracted from the mass of  C. glutamicum  cells). By either increasing the number or the activity of transporter molecules which export fine chemicals from the cell, it may be possible to increase the amount of the produced fine chemical which is present in the extracellular medium, thus permitting greater ease of harvesting and purification. Conversely, in order to efficiently overproduce one or more fine chemicals, increased amounts of the cofactors, precursor molecules, and intermediate compounds for the appropriate biosynthetic pathways are required. Therefore, by increasing the number and/or activity of transporter proteins involved in the import of nutrients, such as carbon sources (i.e., sugars), nitrogen sources (i.e., amino acids, ammonium salts), phosphate, and sulfur, it may be possible to improve the production of a fine chemical, due to the removal of any nutrient supply limitations on the biosynthetic process. Further, fatty acids and lipids are themselves desirable fine chemicals, so by optimizing the activity or increasing the number of one or more MCT proteins of the invention which participate in the biosynthesis of these compounds, or by impairing the activity of one or more MCT proteins which are involved in the degradation of these compounds, it may be possible to increase the yield, production, and/or efficiency of production of fatty acid and lipid molecules from  C. glutamicum.    
      The engineering of one or more MCT genes of the invention may also result in MCT proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from  C. glutamicum . For example, the normal biochemical processes of metabolism result in the production of a variety of waste products (e.g., hydrogen peroxide and other reactive oxygen species) which may actively interfere with these same metabolic processes (for example, peroxynitrite is known to nitrate tyrosine side chains, thereby inactivating some enzymes having tyrosine in the active site (Groves, J. T. (1999)  Curr. Opin. Chem. Biol.  3(2): 226-235). While these waste products are typically excreted, the  C. glutamicum  strains utilized for large-scale fermentative production are optimized for the overproduction of one or more fine chemicals, and thus may produce more waste products than is typical for a wild-type  C. glutamicum.  By optimizing the activity of one or more MCT proteins of the invention which are involved in the export of waste molecules, it may be possible to improve the viability of the cell and to maintain efficient metabolic activity. Also, the presence of high intracellular levels of the desired fine chemical may actually be toxic to the cell, so by increasing the ability of the cell to secrete these compounds, one may improve the viability of the cell.  
      Further, the MCT proteins of the invention may be manipulated such that the relative amounts of various lipid and fatty acid molecules produced are altered. This may have a profound effect on the lipid composition of the membrane of the cell. Since each type of lipid has different physical properties, an alteration in the lipid composition of a membrane may significantly alter membrane fluidity. Changes in membrane fluidity can impact the transport of molecules across the membrane, which, as previously explicated, may modify the export of waste products or the produced fine chemical or the import of necessary nutrients. Such membrane fluidity changes may also profoundly affect the integrity of the cell; cells with relatively weaker membranes are more vulnerable in the large-scale fermentor environment to mechanical stresses which may damage or kill the cell. By manipulating MCT proteins involved in the production of fatty acids and lipids for membrane construction such that the resulting membrane has a membrane composition more amenable to the environmental conditions extant in the cultures utilized to produce fine chemicals, a greater proportion of the  C. glutamicum  cells should survive and multiply. Greater numbers of  C. glutamicum  cells in a culture should translate into greater yields, production, or efficiency of production of the fine chemical from the culture.  
      The aforementioned mutagenesis strategies for MCT proteins to result in increased yields of a fine chemical from  C. glutamicum  are not meant to be limiting; variations on these strategies will be readily apparent to one of ordinary skill in the art. Using such strategies, and incorporating the mechanisms disclosed herein, the nucleic acid and protein molecules of the invention may be utilized to generate  C. glutamicum  or related strains of bacteria expressing mutated MCT nucleic acid and protein molecules such that the yield, production, and/or efficiency of production of a desired compound is improved. This desired compound may be any natural product of  C. glutamicum , which includes the final products of biosynthesis pathways and intermediates of naturally-occurring metabolic pathways, as well as molecules which do not naturally occur in the metabolism of  C. glutamicum , but which are produced by a  C. glutamicum  strain of the invention.  
      This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patent applications, patents, published patent applications, Tables, Appendices, and the sequence listing cited throughout this application are hereby incorporated by reference.  
      Exemplification  
     EXAMPLE 1  
     Preparation of Total Genomic DNA of  Corynebacterium glutamicum  ATCC 13032  
      A culture of  Corynebacterium glutamicum  (ATCC 13032) was grown overnight at 30° C. with vigorous shaking in BHI medium (Difco). The cells were harvested by centrifugation, the supernatant was discarded and the cells were resuspended in 5 ml buffer-I (5% of the original volume of the culture—all indicated volumes have been calculated for 100 ml of culture volume). Composition of buffer-I: 140.34 g/l sucrose, 2.46 g/l MgSO 4 ×7H 2 O, 10 ml/l KH 2 PO 4  solution (100 g/l, adjusted to pH 6.7 with KOH), 50 ml/l M12 concentrate (10 g/l (NH 4 ) 2 SO 4 , 1 g/l NaCl, 2 g/l MgSO 4 ×7 H 2 O, 0.2 g/l CaCl 2 , 0.5 g/l yeast extract (Difco), 10 ml/l trace-elements-mix (200 mg/l FeSO 4 ×H 2 O, 10 mg/l ZnSO 4 ×7 H 2 O, 3 mg/l MnCl 2 ×4 H 2 O, 30 mg/l H 3 BO 3  20 mg/l CoCl 2 ×6 H 2 O, 1 mg/l NiCl 2 ×6 H 2 O, 3 mg/l Na 2 MoO 4 ×2 H 2 O, 500 mg/l complexing agent (EDTA or critic acid), 100 ml/l vitamins-mix (0.2 mg/l biotin, 0.2 mg/l folic acid, 20 mg/l p-amino benzoic acid, 20 mg/l riboflavin, 40 mg/l ca-panthothenate, 140 mg/l nicotinic acid, 40 mg/l pyridoxole hydrochloride, 200 mg/l myo-inositol). Lysozyme was added to the suspension to a final concentration of 2.5 mg/ml. After an approximately 4 h incubation at 37° C., the cell wall was degraded and the resulting protoplasts are harvested by centrifugation. The pellet was washed once with 5 ml buffer-1 and once with 5 ml TE-buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8). The pellet was resuspended in 4 ml TE-buffer and 0.5 ml SDS solution (10%) and 0.5 ml NaCl solution (5 M) are added. After adding of proteinase K to a final concentration of 200 μg/ml, the suspension is incubated for ca. 18 h at 37° C. The DNA was purified by extraction with phenol, phenol-chloroform-isoamylalcohol and chloroform-isoamylalcohol using standard procedures. Then, the DNA was precipitated by adding 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by a 30 min incubation at −20° C. and a 30 min centrifugation at 12,000 rpm in a high speed centrifuge using a SS34 rotor (Sorvall). The DNA was dissolved in 1 ml TE-buffer containing 20 μg/ml RNaseA and dialysed at 4° C. against 1000 ml TE-buffer for at least 3 hours. During this time, the buffer was exchanged 3 times. To aliquots of 0.4 ml of the dialysed DNA solution, 0.4 ml of 2 M LiCl and 0.8 ml of ethanol are added. After a 30 min incubation at −20° C., the DNA was collected by centrifugation (13,000 rpm, Biofuge Fresco, Heraeus, Hanau, Germany). The DNA pellet was dissolved in TE-buffer. DNA prepared by this procedure could be used for all purposes, including southern blotting or construction of genomic libraries.  
     EXAMPLE 2  
     Construction of Genomic Libraries in  Escherichia coli  of  Corynebacterium glutamicum  ATCC13032.  
      Using DNA prepared as described in Example 1, cosmid and plasmid libraries were constructed according to known and well established methods (see e.g., Sambrook, J. et al. (1989) “Molecular Cloning: A Laboratory Manual”, Cold Spring Harbor Laboratory Press, or Ausubel, F. M. et al. (1994) “Current Protocols in Molecular Biology”, John Wiley &amp; Sons.)  
      Any plasmid or cosmid could be used. Of particular use were the plasmids pBR322 (Sutcliffe, J. G. (1979)  Proc. Natl. Acad. Sci. USA,  75:3737-3741); pACYC177 (Change &amp; Cohen (1978)  J. Bacteriol  134:1141-1156), plasmids of the pBS series (pBSSK+, pBSSK− and others; Stratagene, LaJolla, USA), or cosmids as SuperCos1 (Stratagene, LaJolla, USA) or Lorist6 (Gibson, T. J., Rosenthal A. and Waterson, R. H. (1987)  Gene  53:283-286. Gene libraries specifically for use in  C. glutamicum  may be constructed using plasmid pSL109 (Lee, H.-S. and A. J. Sinskey (1994)  J. Microbiol. Biotechnol.  4: 256-263).  
     EXAMPLE 3  
     DNA Sequencing and Computational Functional Analysis  
      Genomic libraries as described in Example 2 were used for DNA sequencing according to standard methods, in particular by the chain termination method using ABI377 sequencing machines (see e.g., Fleischman, R. D. et al. (1995) “Whole-genome Random Sequencing and Assembly of Haemophilus Influenzae Rd.,  Science,  269:496-512). Sequencing primers with the following nucleotide sequences were used: 5′-GGAAACAGTATGACCATG-3′ or 5′-GTAAAACGACGGCCAGT-3′.  
     EXAMPLE 4  
     In vivo Mutagenesis  
      In vivo mutagenesis of  Corynebacterium glutamicum  can be performed by passage of plasmid (or other vector) DNA through  E. coli  or other microorganisms (e.g.  Bacillus  spp. or yeasts such as  Saccharomyces cerevisiae ) which are impaired in their capabilities to maintain the integrity of their genetic information. Typical mutator strains have mutations in the genes for the DNA repair system (e.g., mutHLS, mutD, mutT, etc.; for reference, see Rupp, W. D. (1996) DNA repair mechanisms, in:  Escherichia coli  and  Salmonella , p. 2277-2294, ASM: Washington.) Such strains are well known to those of ordinary skill in the art. The use of such strains is illustrated, for example, in Greener, A. and Callahan, M. (1994)  Strategies  7: 32-34.  
     EXAMPLE 5  
     DNA Transfer Between  Escherichia coli  and  Corynebacterium glutamicum    
      Several  Corynebacterium  and  Brevibacterium  species contain endogenous plasmids (as e.g., pHM1519 or pBL1) which replicate autonomously (for review see, e.g., Martin, J. F. et al. (1987)  Biotechnology,  5:137-146). Shuttle vectors for  Escherichia coli  and  Corynebacterium glutamicum  can be readily constructed by using standard vectors for  E. coli  (Sambrook, J. et al. (1989), “Molecular Cloning: A Laboratory Manual”, Cold Spring Harbor Laboratory Press or Ausubel, F. M. et al. (1994) “Current Protocols in Molecular Biology”, John Wiley &amp; Sons) to which a origin or replication for and a suitable marker from  Corynebacterium glutamicum  is added. Such origins of replication are preferably taken from endogenous plasmids isolated from  Corynebacterium  and  Brevibacterium  species. Of particular use as transformation markers for these species are genes for kanamycin resistance (such as those derived from the Tn5 or Tn903 transposons) or chloramphenicol (Winnacker, E. L. (1987) “From Genes to Clones—Introduction to Gene Technology, VCH, Weinheim). There are numerous examples in the literature of the construction of a wide variety of shuttle vectors which replicate in both  E. coli  and  C. glutamicum , and which can be used for several purposes, including gene over-expression (for reference, see e.g., Yoshihama, M. et al. (1985)  J. Bacteriol.  162:591-597, Martin J. F. et al. (1987)  Biotechnology,  5:137-146 and Eikmanns, B. J. et al. (1991)  Gene,  102:93-98).  
      Using standard methods, it is possible to clone a gene of interest into one of the shuttle vectors described above and to introduce such a hybrid vectors into strains of  Corynebacterium glutamicum.  Transformation of  C. glutamicum  can be achieved by protoplast transformation (Kastsumata, R. et al. (1984)  J. Bacteriol.  159306-311), electroporation (Liebl, E. et al. (1989)  FEMS Microbiol. Letters,  53:399-303) and in cases where special vectors are used, also by conjugation (as described e.g. in Schäfer, A et al. (1990)  J. Bacteriol.  172:1663-1666). It is also possible to transfer the shuttle vectors for  C. glutamicum  to  E. coli  by preparing plasmid DNA from  C. glutamicum  (using standard methods well-known in the art) and transforming it into  E. coli . This transformation step can be performed using standard methods, but it is advantageous to use an Mcr-deficient  E. coli  strain, such as NM522 (Gough &amp; Murray (1983)  J. Mol. Biol.  166:1-19).  
      Genes may be overexpressed in  C. glutamicum  strains using plasmids which comprise pCG1 (U.S. Pat. No. 4,617,267) or fragments thereof, and optionally the gene for kanamycin resistance from TN903 (Grindley, N. D. and Joyce, C. M. (1980)  Proc. Natl. Acad. Sci. USA  77(12): 7176-7180). In addition, genes may be overexpressed in  C. glutamicum  strains using plasmid pSL109 (Lee, H.-S. and A. J. Sinskey (1994)  J. Microbiol. Biotechnol.  4: 256-263).  
      Aside from the use of replicative plasmids, gene overexpression can also be achieved by integration into the genome. Genomic integration in  C. glutamicum  or other  Corynebacterium  or  Brevibacterium  species may be accomplished by well-known methods, such as homologous recombination with genomic region(s), restriction endonuclease mediated integration (REMI) (see, e.g., DE Patent 19823834), or through the use of transposons. It is also possible to modulate the activity of a gene of interest by modifying the regulatory regions (e.g., a promoter, a repressor, and/or an enhancer) by sequence modification, insertion, or deletion using site-directed methods (such as homologous recombination) or methods based on random events (such as transposon mutagenesis or REMI). Nucleic acid sequences which function as transcriptional terminators may also be inserted 3′ to the coding region of one or more genes of the invention; such terminators are well-known in the art and are described, for example, in Winnacker, E. L. (1987) From Genes to Clones—Introduction to Gene Technology. VCH: Weinheim.  
     EXAMPLE 6  
     Assessment of the Expression of the Mutant Protein  
      Observations of the activity of a mutated protein in a transformed host cell rely on the fact that the mutant protein is expressed in a similar fashion and in a similar quantity to that of the wild-type protein. A useful method to ascertain the level of transcription of the mutant gene (an indicator of the amount of mRNA available for translation to the gene product) is to perform a Northern blot (for reference see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: N.Y.), in which a primer designed to bind to the gene of interest is labeled with a detectable tag (usually radioactive or chemiluminescent), such that when the total RNA of a culture of the organism is extracted, run on gel, transferred to a stable matrix and incubated with this probe, the binding and quantity of binding of the probe indicates the presence and also the quantity of mRNA for this gene. This information is evidence of the degree of transcription of the mutant gene. Total cellular RNA can be prepared from  Corynebacterium glutamicum  by several methods, all well-known in the art, such as that described in Bormann, E. R. et al. (1992)  Mol. Microbiol.  6: 317-326.  
      To assess the presence or relative quantity of protein translated from this mRNA, standard techniques, such as a Western blot, may be employed (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: N.Y.). In this process, total cellular proteins are extracted, separated by gel electrophoresis, transferred to a matrix such as nitrocellulose, and incubated with a probe, such as an antibody, which specifically binds to the desired protein. This probe is generally tagged with a chemiluminescent or colorimetric label which may be readily detected. The presence and quantity of label observed indicates the presence and quantity of the desired mutant protein present in the cell.  
     EXAMPLE 7  
     Growth of Genetically Modified  Corynebacterium glutamicum —Media and Culture Conditions  
      Genetically modified  Corynebacteria  are cultured in synthetic or natural growth media. A number of different growth media for  Corynebacteria  are both well-known and readily available (Lieb et al. (1989)  Appl. Microbiol. Biotechnol.,  32:205-210; von der Osten et al. (1998)  Biotechnology Letters,  11:11-16; Patent DE 4,120,867; Liebl (1992) “The Genus  Corynebacterium, in: The Procaryotes, Volume II, Balows, A. et al., eds. Springer-Verlag). These media consist of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars, such as mono-, di-, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. It is also possible to supply sugar to the media via complex compounds such as molasses or other by-products from sugar refinement. It can also be advantageous to supply mixtures of different carbon sources. Other possible carbon sources are alcohols and organic acids, such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are usually organic or inorganic nitrogen compounds, or materials which contain these compounds. Exemplary nitrogen sources include ammonia gas or ammonia salts, such as NH   4 Cl or (NH 4 ) 2 SO 4 , NH 4 OH, nitrates, urea, amino acids or complex nitrogen sources like corn steep liquor, soy bean flour, soy bean protein, yeast extract, meat extract and others.  
      Inorganic salt compounds which may be included in the media include the chloride-, phosphorous- or sulfate- salts of calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc, copper and iron. Chelating compounds can be added to the medium to keep the metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols, like catechol or protocatechuate, or organic acids, such as citric acid. It is typical for the media to also contain other growth factors, such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamin, folic acid, nicotinic acid, pantothenate and pyridoxin. Growth factors and salts frequently originate from complex media components such as yeast extract, molasses, corn steep liquor and others. The exact composition of the media compounds depends strongly on the immediate experiment and is individually decided for each specific case. Information about media optimization is available in the textbook “Applied Microbiol. Physiology, A Practical Approach (eds. P. M. Rhodes, P. F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 3). It is also possible to select growth media from commercial suppliers, like standard 1 (Merck) or BHI (grain heart infusion, DIFCO) or others.  
      All medium components are sterilized, either by heat (20 minutes at 1.5 bar and 121° C.) or by sterile filtration. The components can either be sterilized together or, if necessary, separately. All media components can be present at the beginning of growth, or they can optionally be added continuously or batchwise.  
      Culture conditions are defined separately for each experiment. The temperature should be in a range between 15° C. and 45° C. The temperature can be kept constant or can be altered during the experiment. The pH of the medium should be in the range of 5 to 8.5, preferably around 7.0, and can be maintained by the addition of buffers to the media. An exemplary buffer for this purpose is a potassium phosphate buffer. Synthetic buffers such as MOPS, HEPES, ACES and others can alternatively or simultaneously be used. It is also possible to maintain a constant culture pH through the addition of NaOH or NH 4 OH during growth. If complex medium components such as yeast extract are utilized, the necessity for additional buffers may be reduced, due to the fact that many complex compounds have high buffer capacities. If a fermentor is utilized for culturing the micro-organisms, the pH can also be controlled using gaseous ammonia.  
      The incubation time is usually in a range from several hours to several days. This time is selected in order to permit the maximal amount of product to accumulate in the broth. The disclosed growth experiments can be carried out in a variety of vessels, such as microtiter plates, glass tubes, glass flasks or glass or metal fermentors of different sizes. For screening a large number of clones, the microorganisms should be cultured in microtiter plates, glass tubes or shake flasks, either with or without baffles. Preferably 100 ml shake flasks are used, filled with 10% (by volume) of the required growth medium. The flasks should be shaken on a rotary shaker (amplitude 25 mm) using a speed-range of 100-300 rpm. Evaporation losses can be diminished by the maintenance of a humid atmosphere; alternatively, a mathematical correction for evaporation losses should be performed.  
      If genetically modified clones are tested, an unmodified control clone or a control clone containing the basic plasmid without any insert should also be tested. The medium is inoculated to an OD 600  of 0.5-1.5 using cells grown on agar plates, such as CM plates (10 g/l glucose, 2,5 g/l NaCl, 2 g/l urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l NaCl, 2 g/l urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l agar, pH 6.8 with 2M NaOH) that had been incubated at 30° C. Inoculation of the media is accomplished by either introduction of a saline suspension of  C. glutamicum  cells from CM plates or addition of a liquid preculture of this bacterium.  
     EXAMPLE 8  
     In vitro Analysis of the Function of Mutant Proteins  
      The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one of ordinary skill in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, applications and examples for the determination of many enzyme activities may be found, for example, in the following references: Dixon, M., and Webb, E. C., (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism. Freeman: New York; Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San Francisco; Price, N. C., Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ. Press: Oxford; Boyer, P. D., ed. (1983) The Enzymes, 3 rd  ed. Academic Press: New York; Bisswanger, H., (1994) Enzymkinetik, 2 nd  ed. VCH: Weinheim (ISBN 3527300325); Bergmeyer, H. U., Bergmeyer, J., Gralβl, M., eds. (1983-1986) Methods of Enzymatic Analysis, 3 rd  ed., vol. I-XII, Verlag Chemie: Weinheim; and Ullmann&#39;s Encyclopedia of Industrial Chemistry (1987) vol. A9, “Enzymes”. VCH: Weinheim, p. 352-363.  
      The activity of proteins which bind to DNA can be measured by several well-established methods, such as DNA band-shift assays (also called gel retardation assays). The effect of such proteins on the expression of other molecules can be measured using reporter gene assays (such as that described in Kolmar, H. et al. (1995)  EMBO J.  14: 3895-3904 and references cited therein). Reporter gene test systems are well known and established for applications in both pro- and eukaryotic cells, using enzymes such as beta-galactosidase, green fluorescent protein, and several others.  
      The determination of activity of membrane-transport proteins can be performed according to techniques such as those described in Gennis, R. B. (1989) “Pores, Channels and Transporters”, in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 85-137; 199-234; and 270-322.  
     EXAMPLE 9  
     Analysis of Impact of Mutant Protein on the Production of the Desired Product  
      The effect of the genetic modification in  C. glutamicum  on production of a desired compound (such as an amino acid) can be assessed by growing the modified microorganism under suitable conditions (such as those described above) and analyzing the medium and/or the cellular component for increased production of the desired product (i.e., an amino acid). Such analysis techniques are well known to one of ordinary skill in the art, and include spectroscopy, thin layer chromatography, staining methods of various kinds, enzymatic and microbiological methods, and analytical chromatography such as high performance liquid chromatography (see, for example, Ullman, Encyclopedia of Industrial Chemistry, vol. A2, p. 89-90 and p. 443-613, VCH: Weinheim (1985); Fallon, A. et al., (1987) “Applications of HPLC in Biochemistry” in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17; Rehm et al. (1993) Biotechnology, vol. 3, Chapter III: “Product recovery and purification”, page 469-714, VCH: Weinheim; Belter, P. A. et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons; Kennedy, J. F. and Cabral, J. M. S. (1992) Recovery processes for biological materials, John Wiley and Sons; Shaeiwitz, J. A. and Henry, J. D. (1988) Biochemical separations, in: Ulmann&#39;s Encyclopedia of Industrial Chemistry, vol. B3, Chapter 11, page 1-27, VCH: Weinheim; and Dechow, F. J. (1989) Separation and purification techniques in biotechnology, Noyes Publications.)  
      In addition to the measurement of the final product of fermentation, it is also possible to analyze other components of the metabolic pathways utilized for the production of the desired compound, such as intermediates and side-products, to determine the overall efficiency of production of the compound. Analysis methods include measurements of nutrient levels in the medium (e.g., sugars, hydrocarbons, nitrogen sources, phosphate, and other ions), measurements of biomass composition and growth, analysis of the production of common metabolites of biosynthetic pathways, and measurement of gasses produced during fermentation. Standard methods for these measurements are outlined in Applied Microbial Physiology, A Practical Approach, P. M. Rhodes and P. F. Stanbury, eds., IRL Press, p. 103-129; 131-163; and 165-192 (ISBN: 0199635773) and references cited therein.  
     EXAMPLE 10  
     Purification of the Desired Product from  C. glutamicum  Culture  
      Recovery of the desired product from the  C. glutamicum  cells or supernatant of the above-described culture can be performed by various methods well known in the art. If the desired product is not secreted from the cells, the cells can be harvested from the culture by low-speed centrifugation, the cells can be lysed by standard techniques, such as mechanical force or sonication. The cellular debris is removed by centrifligation, and the supernatant fraction containing the soluble proteins is retained for further purification of the desired compound. If the product is secreted from the  C. glutamicum  cells, then the cells are removed from the culture by low-speed centrifugation, and the supernate fraction is retained for further purification.  
      The supernatant fraction from either purification method is subjected to chromatography with a suitable resin, in which the desired molecule is either retained on a chromatography resin while many of the impurities in the sample are not, or where the impurities are retained by the resin while the sample is not. Such chromatography steps may be repeated as necessary, using the same or different chromatography resins. One of ordinary skill in the art would be well-versed in the selection of appropriate chromatography resins and in their most efficacious application for a particular molecule to be purified. The purified product may be concentrated by filtration or ultrafiltration, and stored at a temperature at which the stability of the product is maximized.  
      There are a wide array of purification methods known to the art and the preceding method of purification is not meant to be limiting. Such purification techniques are described, for example, in Bailey, J. E. &amp; Ollis, D. F. Biochemical Engineering Fundamentals, McGraw-Hill: N.Y. (1986).  
      The identity and purity of the isolated compounds may be assessed by techniques standard in the art. These include high-performance liquid chromatography (HPLC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzymatic assay, or microbiologically. Such analysis methods are reviewed in: Patek et al. (1994)  Appl. Environ. Microbiol.  60: 133-140; Malakhova et al. (1996)  Biotekhnologiya  11: 27-32; and Schmidt et al. (1998)  Bioprocess Engineer.  19: 67-70. Ulmann&#39;s Encyclopedia of Industrial Chemistry, (1996) vol. A27, VCH: Weinheim, p. 89-90, p. 521-540, p. 540-547, p. 559-566, 575-581 and p. 581-587; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al. (1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.  
     EXAMPLE 11  
     Analysis of the Gene Sequences of the Invention  
      The comparison of sequences and determination of percent homology between two sequences are art-known techniques, and can be accomplished using a mathematical algorithm, such as the algorithm of Karlin and Altschul (1990)  Proc. Natl. Acad Sci . USA 87:2264-68, modified as in Karlin and Altschul (1993)  Proc. Natl. Acad. Sci . USA 90:5873-77. Such an algorithm is incorporated into the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990)  J. Mol. Biol.  215:403-10. BLAST nucleotide searches can be performed with the NBLAST program, score=100, wordlength=12 to obtain nucleotide sequences homologous to MCT nucleic acid molecules of the invention. BLAST protein searches can be performed with the XBLAST program, score=50, wordlength=3 to obtain amino acid sequences homologous to MCT protein molecules of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., (1997)  Nucleic Acids Res.  25(17):3389-3402. When utilizing BLAST and Gapped BLAST programs, one of ordinary skill in the art will know how to optimize the parameters of the program (e.g., XBLAST and NBLAST) for the specific sequence being analyzed.  
      Another example of a mathematical algorithm utilized for the comparison of sequences is the algorithm of Meyers and Miller ((1988)  Comput. Appl. Biosci.  4: 11-17). Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM 120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, and include ADVANCE and ADAM. described in Torelli and Robotti (1994)  Comput. Appl. Biosci.  10:3-5; and FASTA, described in Pearson and Lipman (1988)  P.N.A.S.  85:2444-8.  
      The percent homology between two amino acid sequences can also be accomplished using the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blosum 62 matrix or a PAM250 matrix, and a gap weight of 12, 10, 8, 6, or 4 and a length weight of 2, 3, or 4. The percent homology between two nucleic acid sequences can be accomplished using the GAP program in the GCG software package, using standard parameters, such as a gap weight of 50 and a length weight of 3.  
      A comparative analysis of the gene sequences of the invention with those present in Genbank has been performed using techniques known in the art (see, e.g., Bexevanis and Ouellette, eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. John Wiley and Sons: New York). The gene sequences of the invention were compared to genes present in Genbank in a three-step process. In a first step, a BLASTN analysis (e.g., a local alignment analysis) was performed for each of the sequences of the invention against the nucleotide sequences present in Genbank, and the top 500 hits were retained for further analysis. A subsequent FASTA search (e.g., a combined local and global alignment analysis, in which limited regions of the sequences are aligned) was performed on these 500 hits. Each gene sequence of the invention was subsequently globally aligned to each of the top three FASTA hits, using the GAP program in the GCG software package (using standard parameters). In order to obtain correct results, the length of the sequences extracted from Genbank were adjusted to the length of the query sequences by methods well-known in the art. The results of this analysis are set forth in Table 4. The resulting data is identical to that which would have been obtained had a GAP (global) analysis alone been performed on each of the genes of the invention in comparison with each of the references in Genbank, but required significantly reduced computational time as compared to such a database-wide GAP (global) analysis. Sequences of the invention for which no alignments above the cutoff values were obtained are indicated on Table 4 by the absence of alignment information. It will further be understood by one of ordinary skill in the art that the GAP alignment homology percentages set forth in Table 4 under the heading “% homology (GAP)” are listed in the European numerical format, wherein a ‘,’ represents a decimal point. For example, a value of “40,345” in this column represents “40.345%”.  
     EXAMPLE 12  
     Construction and Operation of DNA Microarrays  
      The sequences of the invention may additionally be used in the construction and application of DNA microarrays (the design, methodology, and uses of DNA arrays are well known in the art, and are described, for example, in Schena, M. et al. (1995)  Science  270: 467-470; Wodicka, L. et al. (1997)  Nature Biotechnology  15: 1359-1367; DeSaizieu, A. et al. (1998)  Nature Biotechnology  16: 45-48; and DeRisi, J. L. et al. (1997)  Science  278: 680-686).  
      DNA microarrays are solid or flexible supports consisting of nitrocellulose, nylon, glass, silicone, or other materials. Nucleic acid molecules may be attached to the surface in an ordered manner. After appropriate labeling, other nucleic acids or nucleic acid mixtures can be hybridized to the immobilized nucleic acid molecules, and the label may be used to monitor and measure the individual signal intensities of the hybridized molecules at defined regions. This methodology allows the simultaneous quantification of the relative or absolute amount of all or selected nucleic acids in the applied nucleic acid sample or mixture. DNA microarrays, therefore, permit an analysis of the expression of multiple (as many as 6800 or more) nucleic acids in parallel (see, e.g., Schena, M. (1996)  BioEssays  18(5): 427-431).  
      The sequences of the invention may be used to design oligonucleotide primers which are able to amplify defined regions of one or more  C. glutamicum  genes by a nucleic acid amplification reaction such as the polymerase chain reaction. The choice and design of the 5′ or 3′ oligonucleotide primers or of appropriate linkers allows the covalent attachment of the resulting PCR products to the surface of a support medium described above (and also described, for example, Schena, M. et al. (1995)  Science  270: 467-470).  
      Nucleic acid microarrays may also be constructed by in situ oligonucleotide synthesis as described by Wodicka, L. et al. (1997)  Nature Biotechnology  15: 1359-1367. By photolithographic methods, precisely defined regions of the matrix are exposed to light. Protective groups which are photolabile are thereby activated and undergo nucleotide addition, whereas regions that are masked from light do not undergo any modification. Subsequent cycles of protection and light activation permit the synthesis of different oligonucleotides at defined positions. Small, defined regions of the genes of the invention may be synthesized on microarrays by solid phase oligonucleotide synthesis.  
      The nucleic acid molecules of the invention present in a sample or mixture of nucleotides may be hybridized to the microarrays. These nucleic acid molecules can be labeled according to standard methods. In brief, nucleic acid molecules (e.g., mRNA molecules or DNA molecules) are labeled by the incorporation of isotopically or fluorescently labeled nucleotides, e.g., during reverse transcription or DNA synthesis. Hybridization of labeled nucleic acids to microarrays is described (e.g., in Schena, M. et al. (1995) supra; Wodicka, L. et al. (1997), supra; and DeSaizieu A. et al. (1998), supra). The detection and quantification of the hybridized molecule are tailored to the specific incorporated label. Radioactive labels can be detected, for example, as described in Schena, M. et al. (1995) supra) and fluorescent labels may be detected, for example, by the method of Shalon et al. (1996)  Genome Research  6: 639-645).  
      The application of the sequences of the invention to DNA microarray technology, as described above, permits comparative analyses of different strains of  C. glutamicum  or other  Corynebacteria . For example, studies of inter-strain variations based on individual transcript profiles and the identification of genes that are important for specific and/or desired strain properties such as pathogenicity, productivity and stress tolerance are facilitated by nucleic acid array methodologies. Also, comparisons of the profile of expression of genes of the invention during the course of a fermentation reaction are possible using nucleic acid array technology.  
     EXAMPLE 13  
     Analysis of the Dynamics of Cellular Protein Populations (Proteomics)  
      The genes, compositions, and methods of the invention may be applied to study the interactions and dynamics of populations of proteins, termed ‘proteomics’. Protein populations of interest include, but are not limited to, the total protein population of  C. glutamicum  (e.g., in comparison with the protein populations of other organisms), those proteins which are active under specific environmental or metabolic conditions (e.g., during fermentation, at high or low temperature, or at high or low pH), or those proteins which are active during specific phases of growth and development.  
      Protein populations can be analyzed by various well-known techniques, such as gel electrophoresis. Cellular proteins may be obtained, for example, by lysis or extraction, and may be separated from one another using a variety of electrophoretic techniques. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins largely on the basis of their molecular weight. Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAGE) separates proteins by their isoelectric point (which reflects not only the amino acid sequence but also posttranslational modifications of the protein). Another, more preferred method of protein analysis is the consecutive combination of both IEF-PAGE and SDS-PAGE, known as 2-D-gel electrophoresis (described, for example, in Hermann et al. (1998)  Electrophoresis  19: 3217-3221; Fountoulakis et al. (1998)  Electrophoresis  19: 1193-1202; Langen et al. (1997)  Electrophoresis  18: 1184-1192; Antelmann et al. (1997)  Electrophoresis  18: 1451-1463). Other separation techniques may also be utilized for protein separation, such as capillary gel electrophoresis; such techniques are well known in the art.  
      Proteins separated by these methodologies can be visualized by standard techniques, such as by staining or labeling. Suitable stains are known in the art, and include Coomassie Brilliant Blue, silver stain, or fluorescent dyes such as Sypro Ruby (Molecular Probes). The inclusion of radioactively labeled amino acids or other protein precursors (e.g.,  35 S-methionine,  35 S-cysteine,  14 C-labelled amino acids,  15 N-amino acids,  15 NO 3  or  15 NH 4   +  or  13 C-labelled amino acids) in the medium of  C. glutamicum  permits the labeling of proteins from these cells prior to their separation. Similarly, fluorescent labels may be employed. These labeled proteins can be extracted, isolated and separated according to the previously described techniques.  
      Proteins visualized by these techniques can be further analyzed by measuring the amount of dye or label used. The amount of a given protein can be determined quantitatively using, for example, optical methods and can be compared to the amount of other proteins in the same gel or in other gels. Comparisons of proteins on gels can be made, for example, by optical comparison, by spectroscopy, by image scanning and analysis of gels, or through the use of photographic films and screens. Such techniques are well-known in the art.  
      To determine the identity of any given protein, direct sequencing or other standard techniques may be employed. For example, N- and/or C-terminal amino acid sequencing (such as Edman degradation) may be used, as may mass spectrometry (in particular MALDI or ESI techniques (see, e.g., Langen et al. (1997)  Electrophoresis  18: 1184-1192)). The protein sequences provided herein can be used for the identification of  C. glutamicum  proteins by these techniques.  
      The information obtained by these methods can be used to compare patterns of protein presence, activity, or modification between different samples from various biological conditions (e.g., different organisms, time points of fermentation, media conditions, or different biotopes, among others). Data obtained from such experiments alone, or in combination with other techniques, can be used for various applications, such as to compare the behavior of various organisms in a given (e.g., metabolic) situation, to increase the productivity of strains which produce fine chemicals or to increase the efficiency of the production of fine chemicals.  
      Equivalents  
      Those of ordinary skill in the art will recognize, or will be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.  
               TABLE 1                          GENES IN THE APPLICATION                                         Nucleic   Amino                           Acid   Acid       SEQ   SEQ       ID   ID   Identification       NT   NT       NO   NO   Code   Contig.   Start   Stop   Function                                                 1   2   RXN03097   VV0062   3   557   AMMONIUM TRANSPORT SYSTEM       3   4   RXA02099   GR00630   6198   6470   AMMONIUM TRANSPORT SYSTEM       5   6   RXA00104   GR00014   15895   16650   CYSQ PROTEIN, ammonium transport protein                 Polyketide Synthesis                                         7   8   RXA01420   GR00416   775   17   4″-MYCAROSYL ISOVALERYL-COA TRANSFERASE (EC 2.—.—.—)       9   10   RXN02581   VV0098   30482   28623   POLYKETIDE SYNTHASE       11   12   F RXA02581   GR00741   1   1527   POLYKETIDE SYNTHASE       13   14   RXA02582   GR00741   1890   6719   PROBABLE POLYKETIDE SYNTHASE CY338.20       15   16   RXA01138   GR00318   1656   2072   ACTINORHODIN POLYKETIDE DIMERASE (EC —.—.—.—)       17   18   RXA01980   GR00573   1470   838   POLYKETIDE CYCLASE       19   20   RXN01007   VV0021   2572   866   FRNA       21   22   RXN00784   VV0103   27531   28265   FRNE                 Fatty acid and lipid synthesis                                         23   24   RXA02335   GR00672   550   2322   BIOTIN CARBOXYLASE (EC 6.3.4.14)       25   26   RXA02173   GR00641   7473   8924   ACETYL-COENZYME A CARBOXYLASE CARBOXYL                               TRANSFERASE SUBUNIT BETA (EC 6.4.1.2)       27   28   RXA01764   GR00500   2178   3110   3-OXOACYL-[ACYL-CARRIER PROTEIN] REDUCTASE (EC 1.1.1.100)       29   30   RXN02487   VV0007   6367   4664   LONG-CHAIN-FATTY-ACID—COA LIGASE (EC 6.2.1.3)       31   32   F RXA02487   GR00718   4937   4650   LONG-CHAIN-FATTY-ACID—COA LIGASE (EC 6.2.1.3)       33   34   F RXA02490   GR00720   817   5   LONG-CHAIN-FATTY-ACID—COA LIGASE (EC 6.2.1.3)       35   36   RXA01467   GR00422   920   1210   ACYL CARRIER PROTEIN       37   38   RXA00796   GR00212   202   5   Acyl carrier protein phosphodiesterase       39   40   RXA01897   GR00544   617   1159   Acyl carrier protein phosphodiesterase       41   42   RXN02809   VV0342   380   6   Acyl carrier protein phosphodiesterase       43   44   F RXA02809   GR00790   277   5   Acyl carrier protein phosphodiesterase       45   46   RXN00113   VV0129   103   5724   FATTY ACID SYNTHASE (EC 2.3.1.85) [INCLUDES: EC 2.3.1.38;                               EC 2.3.1.39; EC 2.3.1.41;       47   48   F RXA00113   GR00017   2   3295   FATTY-ACID SYNTHASE (EC 2.3.1.85)       49   50   RXN03111   VV0084   6040   5   FATTY ACID SYNTHASE (EC 2.3.1.85) [INCLUDES: EC 2.3.1.38;                               EC 2.3.1.39; EC 2.3.1.41; EC 1.1.1.100; EC 4.2.1.61;                               EC 1.3.1.10; EC 3.1.2.14]       51   52   F RXA00158   GR00024   2088   4   FATTY ACID SYNTHASE (EC 2.3.1.85)       53   54   F RXA00572   GR00155   2   3832   FATTY ACID SYNTHASE (EC 2.3.1.85)       55   56   RXA02582   GR00741   1890   6719   PROBABLE POLYKETIDE SYNTHASE CY338.20       57   58   RXA02691   GR00754   15347   14541   FATTY ACYL RESPONSIVE REGULATOR       59   60   RXA00880   GR00242   6213   8057   LONG-CHAIN-FATTY-ACID—COA LIGASE (EC 6.2.1.3)       61   62   RXA01060   GR00296   9566   10489   OMEGA-3 FATTY ACID DESATURASE (EC 1.14.99.—)       63   64   RXN01722   VV0036   2938   1214   MEDIUM-CHAIN-FATTY-ACID—COA LIGASE (EC 6.2.1.—)       65   66   F RXA01722   GR00488   5746   4022   MEDIUM-CHAIN-FATTY-ACID—COA LIGASE (EC 6.2.1.—)       67   68   RXA01644   GR00456   9854   8577   CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE (EC 2.1.1.79)       69   70   RXA02029   GR00618   356   1669   CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE (EC 2.1.1.79)       71   72   RXA01801   GR00509   3396   2380   ENOYL-COA HYDRATASE (EC 4.2.1.17)       73   74   RXN02512   VV0171   16147   15185   LIPID A BIOSYNTHESIS LAUROYL ACYLTRANSFERASE (EC 2.3.1.—)       75   76   F RXA02512   GR00721   3303   4259   LIPID A BIOSYNTHESIS LAUROYL ACYLTRANSFERASE (EC 2.3.1.—)       77   78   RXA00899   GR00245   1599   2864   CARDIOLIPIN SYNTHETASE (EC 2.7.8.—)       79   80   RXN00819   VV0054   18127   19455   ACYL-COA DEHYDROGENASE (EC 1.3.99.—)       81   82   F RXA00819   GR00221   18   1007   ACYL-COA DEHYDROGENASE (EC 1.3.99.—)       83   84   F RXA01766   GR00500   4081   4371   ACYL-COA DEHYDROGENASE (EC 1.3.99.—)       85   86   RXN01762   VV0054   15318   13783   LONG-CHAIN-FATTY-ACID—COA LIGASE (EC 6.2.1.3)       87   88   F RXA01762   GR00500   1272   10   LONG-CHAIN-FATTY-ACID—COA LIGASE (EC 6.2.1.3)       89   90   RXA00681   GR00179   3405   2662   3-OXOACYL-[ACYL-CARRIER PROTEIN] REDUCTASE (EC 1.1.1.100)       91   92   RXA00802   GR00214   3803   4516   3-OXOACYL-[ACYL-CARRIER PROTEIN] REDUCTASE (EC 1.1.1.100)       93   94   RXA02133   GR00639   3   308   3-OXOACYL-[ACYL-CARRIER PROTEIN] REDUCTASE (EC 1.1.1.100)       95   96   RXN01114   VV0182   9118   10341   3-KETOACYL-COA THIOLASE (EC 2.3.1.16)       97   98   F RXA01114   GR00308   2   793   3-KETOACYL-COA THIOLASE (EC 2.3.1.16)       99   100   RXA01894   GR00542   1622   2476   PHOSPHATIDATE CYTIDYLYLTRANSFERASE (EC 2.7.7.41)       101   102   RXA02599   GR00742   3179   3655   PHOSPHATIDYLGLYCEROPHOSPHATASE B (EC 3.1.3.27)       103   104   RXN02638   VV0098   54531   53656   1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (EC 2.3.1.51)       105   106   F RXA02638   GR00749   8   511   1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (EC 2.3.1.51)       107   108   RXA00856   GR00232   720   1256   CDP-DIACYLGLYCEROL—GLYCEROL-3-PHOSPHATE 3-                               PHOSPHATIDYLTRANSFERASE (EC 2.7.8.5)       109   110   RXA02511   GR00721   2621   3277   CDP-DIACYLGLYCEROL—GLYCEROL-3-PHOSPHATE 3-                               PHOSPHATIDYLTRANSFERASE (EC 2.7.8.5)       111   112   RXN02836   VV0102   32818   33372   KETOACYL REDUCTASE HETN (EC 1.3.1.—)       113   114   F RXA02836   GR00827   106   411   KETOACYL REDUCTASE HETN (EC 1.3.1.—)       115   116   RXA02578   GR00740   2438   3541   PUTATIVE ACYLTRANSFERASE       117   118   RXA02150   GR00639   18858   19658   1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (EC 2.3.1.51)       119   120   RXA00607   GR00160   1869   2249   POLY(3-HYDROXYALKANOATE) POLYMERASE (EC 2.3.1.—)       121   122   RXA02397   GR00698   1688   2683   POLY-BETA-HYDROXYBUTYRATE POLYMERASE (EC 2.3.1.—)       123   124   RXN03110   VV0083   16568   17929   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       125   126   F RXA00660   GR00171   1027   5   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       127   128   RXA00801   GR00214   3138   3770   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       129   130   RXA00821   GR00221   1469   2311   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       131   132   RXN02966   VV0143   12056   13462   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       133   134   F RXA01833   GR00517   1666   260   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       135   136   RXA01853   GR00525   5561   5010   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       137   138   RXN02424   VV0116   10570   11169   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       139   140   F RXA02424   GR00706   808   428   HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6)       141   142   RXN00419   VV0112   1024   266   ACETOACETYL-COA REDUCTASE (EC 1.1.1.36)       143   144   F RXA00419   GR00095   3   464   ACETOACETYL-COA REDUCTASE (EC 1.1.1.36)       145   146   F RXA00421   GR00096   565   723   ACETOACETYL-COA REDUCTASE (EC 1.1.1.36)       147   148   RXN02923   VV0088   3301   2564   ACETOACETYL-COA REDUCTASE (EC 1.1.1.36)       149   150   RXN02922   VV0321   11407   10328   ACYL-COA DEHYDROGENASE, SHORT-CHAIN SPECIFIC (EC 1.3.99.2)       151   152   RXN03065   VV0038   6237   6629   HOLO-[ACYL-CARRIER PROTEIN] SYNTHASE (EC 2.7.8.7)       153   154   RXN03132   VV0127   39053   39472   POLY-BETA-HYDROXYBUTYRATE POLYMERASE (EC 2.3.1.—)       155   156   RXN03157   VV0188   1607   1170   LIPOPOLYSACCHARIDE CORE BIOSYNTHESIS PROTEIN KDTB       157   158   RXN00934   VV0171   15181   14099   (AE000805) LPS biosynthesis RfbU related protein [Methanobacterium                               thermoautotrophicum]       159   160   RXN00792   VV0321   10328   9132   ACYL-COA DEHYDROGENASE, SHORT-CHAIN SPECIFIC (EC 1.3.99.2)       161   162   RXN00931   VV0171   13011   12166   ACYL-COA THIOESTERASE II (EC 3.1.2.—)       163   164   F RXA00931   GR00253   4959   4114   thioesterase II       165   166   RXN01421   VV0122   16024   15638   ACYLTRANSFERASE (EC 2.3.1.—)       167   168   RXN02342   VV0078   3460   4266   BIOTIN—[ACETYL-COA-CARBOXYLASE] SYNTHETASE (EC 6.3.4.15)       169   170   RXN00563   VV0038   1   2739   FATTY ACID SYNTHASE (EC 2.3.1.85) [INCLUDES: EC 2.3.1.38;                               EC 2.3.1.39; EC 2.3.1.41; EC 1.1.1.100; EC 4.2.1.61; EC 1.3.1.10; EC 3.1.2.14]       171   172   RXN02168   VV0100   2894   81   FATTY ACID SYNTHASE (EC 2.3.1.85) [INCLUDES: EC 2.3.1.38;                               EC 2.3.1.39; EC 2.3.1.41; EC 1.1.1.100; EC 4.2.1.61; EC 1.3.1.10;                               EC 3.1.2.14]       173   174   RXN01090   VV0155   6483   5686   KETOACYL REDUCTASE HETN (EC 1.3.1.—)       175   176   RXN02062   VV0222   3159   1990   Lipopolysaccharide N-acetylglucosaminyltransferase       177   178   RXN02148   VV0300   16561   17703   Lipopolysaccharide N-acetylglucosaminyltransferase       179   180   RXN02595   VV0098   11098   9935   Lipopolysaccharide N-acetylglucosaminyltransferase       181   182   RXS00148   VV0167   9849   12059   METHYLMALONYL-COA MUTASE ALPHA-SUBUNIT (EC 5.4.99.2)       183   184   RXS00149   VV0167   7995   9842   METHYLMALONYL-COA MUTASE BETA-SUBUNIT (EC 5.4.99.2)       185   186   RXS02106   VV0123   22649   21594   LIPOATE-PROTEIN LIGASE A (EC 6.—.—.—)       187   188   RXS01746   VV0185   934   1686   LIPOATE-PROTEIN LIGASE B (EC 6.—.—.—)       189   190   RXS01747   VV0185   1826   2869   LIPOIC ACID SYNTHETASE       191   192   RXC01748   VV0185   3001   3780   protein involved in lipid metabolism       193   194   RXC00354   VV0135   33604   32792   Cytosolic Protein involved in lipid metabolism       195   196   RXC01749   VV0185   3953   5569   Membrane Spanning Protein involved in lipid metabolism                 Fatty acid degradation                                         197   198   RXA02268   GR00655   2182   3081   LIPASE (EC 3.1.1.3)       199   200   RXA02269   GR00655   3094   4065   LIPASE (EC 3.1.1.3)       201   202   RXA01614   GR00449   8219   7197   LYSOPHOSPHOLIPASE L2 (EC 3.1.1.5)       203   204   RXA01983   GR00573   3559   3053   LIPASE (EC 3.1.1.3)       205   206   RXN02947   VV0078   1319   6   PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3)       207   208   F RXA02320   GR00667   593   6   PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3)       209   210   F RXA02851   GR00851   524   6   PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3)       211   212   RXN02321   VV0078   3291   1663   PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3)       213   214   F RXA02321   GR00667   1380   937   PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3)       215   216   F RXA02343   GR00675   1403   1816   PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3)       217   218   F RXA02850   GR00850   2   493   PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3)       219   220   RXA02583   GR00741   6743   8290   PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3)       221   222   RXA00870   GR00239   809   2320   METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE                               (ACYLATING) (EC 1.2.1.27) 2-Methyl-3-oxopropanoate:                               NAD+ oxidoreductase (CoA-propanoylating)       223   224   RXA01260   GR00367   2381   1200   LIPOAMIDE DEHYDROGENASE COMPONENT (E3) OF BRANCHED-CHAIN                               ALPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4)       225   226   RXA01261   GR00367   2607   2437   LIPOAMIDE DEHYDROGENASE COMPONENT (E3) OF BRANCHED-CHAIN                               ALPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4)       227   228   RXA01136   GR00318   685   1116   ISOVALERYL-COA DEHYDROGENASE (EC 1.3.99.10)       229   230   RXN00559   VV0103   7568   6552   PROTEIN VDLD       231   232   F RXA00559   GR00149   218   6   PROTEIN VDLD       233   234   RXA01580   GR00440   707   6   Glycerophosphoryl diester phosphodiesterase       235   236   RXA02677   GR00754   3119   3877   GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE (EC 3.1.4.46)       237   238   RXS01166   VV0117   18142   16838   EXTRACELLULAR LIPASE PRECURSOR (EC 3.1.1.3)                 Terpenoid biosynthesis                                         239   240   RXA00875   GR00241   2423   1857   ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE (EC 5.3.3.2)       241   242   RXA01292   GR00373   1204   2388   PHYTOENE DEHYDROGENASE (EC 1.3.—.—)       243   244   RXA01293   GR00373   2370   2696   PHYTOENE DEHYDROGENASE (EC 1.3.—.—)       245   246   RXA02310   GR00665   1132   2394   GERANYLGERANYL HYDROGENASE       247   248   RXA02718   GR00758   18539   19585   GERANYLGERANYL PYROPHOSPHATE SYNTHASE (EC 2.5.1.1)       249   250   RXA01067   GR00298   1453   2181   undecaprenyl-diphosphate synthase (EC 2.5.1.31)       251   252   RXA01269   GR00367   20334   19894   UNDECAPRENYL-PHOSPHATE GALACTOSEPHOSPHOTRANSFERASE (EC                               2.7.8.6)       253   254   RXA01205   GR00346   3   533   PUTATIVE UNDECAPRENYL-PHOSPHATE ALPHA-N-                               ACETYLGLUCOSAMINYLTRANSFERASE (EC 2.4.1.—)       255   256   RXA01576   GR00438   8053   8811   DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE (EC 2.4.1.117)       257   258   RXN02309   VV0025   28493   29542   OCTAPRENYL-DIPHOSPHATE SYNTHASE (EC 2.5.1.—)       259   260   F RXA02309   GR00665   978   4   OCTAPRENYL-DIPHOSPHATE SYNTHASE (EC 2.5.1.—)       261   262   RXN00477   VV0086   38905   37262   PHYTOENE DEHYDROGENASE (EC 1.3.—.—)       263   264   F RXA00477   GR00119   13187   11544   PHYTOENE DEHYDROGENASE (EC 1.3.—.—)       265   266   RXA00478   GR00119   14020   13190   PHYTOENE SYNTHASE (EC 2.5.1.—)       267   268   RXA01291   GR00373   345   1277   PHYTOENE SYNTHASE (EC 2.5.1.—)       269   270   RXA00480   GR00119   17444   16329   FARNESYL DIPHOSPHATE SYNTHASE (EC 2.5.1.1) (EC 2.5.1.10)       271   272   RXS01879   VV0105   1505   573   isopentenyl-phosphate kinase (EC 2.7.4.—)       273   274   RXS02023   VV0160   3234   4001   P450 cytochrome, isopentenyltransf, ferridox       275   276   RXS00948   VV0107   4266   5384   12-oxophytodienoate reductase (EC 1.3.1.42)       277   278   RXS02228   VV0068   1876   2778   TRNA DELTA(2)-ISOPENTENYLPYROPHOSPHATE                               TRANSFERASE (EC 2.5.1.8)       279   280   RXC01971   VV0105   4545   3715   Metal-Dependent Hydrolase involved in metabolism of terpenoids       281   282   RXC02697   VV0017   31257   32783   membrane protein involved in metabolism of terpenoids                 ABC-Transporter                                         283   284   RXN01946   VV0228   2   1276   Hypothetical ABC Transporter ATP-Binding Protein       285   286   F RXA01946   GR00559   1849   575   (AL021184) ABC transporter ATP binding protein [Mycobacterium tuberculosis]       287   288   RXN00164   VV0232   1782   94   Hypothetical ABC Transporter ATP-Binding Protein       289   290   F RXA00164   GR00025   1782   94   , P, G, R ATPase subunits of ABC transporters       291   292   RXN00243   VV0057   28915   27899   , P, G, R ATPase subunits of ABC transporters       293   294   F RXA00243   GR00037   930   4   , P, G, R ATPase subunits of ABC transporters       295   296   RXA00259   GR00039   8469   6268   , P, G, R ATPase subunits of ABC transporters       297   298   RXN00410   VV0086   51988   51323   GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ       299   300   F RXA00410   GR00092   829   164   , P, G, R ATPase subunits of ABC transporters       301   302   RXN00456   VV0076   6780   8156   , P, G, R ATPase subunits of ABC transporters       303   304   F RXA00456   GR00114   316   5   , P, G, R ATPase subunits of ABC transporters       305   306   F RXA00459   GR00115   1231   245   , P, G, R ATPase subunits of ABC transporters       307   308   RXN01604   VV0137   8117   7470   , P, G, R ATPase subunits of ABC transporters       309   310   F RXA01604   GR00448   2   607   , P, G, R ATPase subunits of ABC transporters       311   312   RXN02547   VV0057   27726   25588   , P, G, R ATPase subunits of ABC transporters       313   314   F RXA02547   GR00726   22055   19932   , P, G, R ATPase subunits of ABC transporters       315   316   RXN02571   VV0101   12331   13359   MALTOSE/MALTODEXTRIN TRANSPORT ATP-BINDING PROTEIN MALK       317   318   F RXA02571   GR00736   1469   2497   , P, G, R ATPase subunits of ABC transporters       319   320   RXN02074   VV0318   12775   11153   TRANSPORT ATP-BINDING PROTEIN CYDD       321   322   F RXA02074   GR00628   5798   4176   , P, G, R ATPase subunits of ABC transporters       323   324   RXA02095   GR00629   14071   15474   , P, G, R ATPase subunits of ABC transporters       325   326   RXA02225   GR00652   3156   2275   , P, G, R ATPase subunits of ABC transporters       327   328   RXA02253   GR00654   20480   21406   , P, G, R ATPase subunits of ABC transporters       329   330   RXN01881   VV0105   529   95   Hypothetical ABC Transporter ATP-Binding Protein       331   332   F RXA01881   GR00537   3092   3532   ATPase components of ABC transporters with duplicated ATPase domains       333   334   RXA00526   GR00136   1353   664   Hypothetical ABC Transporter ATP-Binding Protein       335   336   RXN00733   VV0132   1647   2531   Hypothetical ABC Transporter ATP-Binding Protein       337   338   F RXA00733   GR00197   411   4   Hypothetical ABC Transporter ATP-Binding Protein       339   340   RXA00735   GR00198   849   181   Hypothetical ABC Transporter ATP-Binding Protein       341   342   RXA00878   GR00242   3733   1871   Hypothetical ABC Transporter ATP-Binding Protein       343   344   RXN01191   VV0169   10478   12067   Hypothetical ABC Transporter ATP-Binding Protein       345   346   F RXA01191   GR00341   1571   165   Hypothetical ABC Transporter ATP-Binding Protein       347   348   RXN01212   VV0169   3284   4207   Hypothetical ABC Transporter ATP-Binding Protein       349   350   F RXA01212   GR00350   1   813   Hypothetical ABC Transporter ATP-Binding Protein       351   352   RXA02749   GR00764   4153   5028   Hypothetical ABC Transporter ATP-Binding Protein       353   354   RXA02224   GR00652   2271   475   Hypothetical ABC Transporter ATP-Binding Protein       355   356   RXN01602   VV0229   1109   2638   Hypothetical ABC Transporter ATP-Binding Protein       357   358   RXN02515   VV0087   962   1717   Hypothetical ABC Transporter ATP-Binding Protein       359   360   RXN00525   VV0079   26304   27566   Hypothetical ABC Transporter Permease Protein       361   362   RXN02096   VV0126   20444   22135   Hypothetical ABC Transporter Permease Protein       363   364   RXN00412   VV0086   53923   52844   Hypothetical Amino Acid ABC Transporter ATP-Binding Protein       365   366   RXN00411   VV0086   52844   52170   Hypothetical Amino Acid ABC Transporter Permease Protein       367   368   RXN02614   VV0313   5964   5236   TAURINE TRANSPORT ATP-BINDING PROTEIN TAUB       369   370   RXN02613   VV0313   5223   4267   TAURINE-BINDING PERIPLASMIC PROTEIN PRECURSOR       371   372   RXN00368   VV0226   2300   726   SPERMIDINE/PUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA       373   374   F RXA00368   GR00076   1   579   SPERMIDINE/PUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA       375   376   F RXA00370   GR00077   6   803   SPERMIDINE/PUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA       377   378   RXN01285   VV0215   1780   1055   FERRIC ENTEROBACTIN TRANSPORT ATP-BINDING PROTEIN FEPC       379   380   RXN00523   VV0194   1363   338   FERRIC ENTEROBACTIN TRANSPORT PROTEIN FEPG       381   382   RXN01142   VV0077   5805   6302   NITRATE TRANSPORT ATP-BINDING PROTEIN NRTD       383   384   RXN01141   VV0077   4644   5468   NITRATE TRANSPORT PROTEIN NRTA       385   386   RXN01002   VV0106   8858   8055   PHOSPHONATES TRANSPORT ATP-BINDING PROTEIN PHNC       387   388   RXN01000   VV0106   7252   6407   PHOSPHONATES TRANSPORT SYSTEM PERMEASE PROTEIN PHNE       389   390   RXN01732   VV0106   9944   8895   PHOSPHONATES-BINDING PERIPLASMIC PROTEIN PRECURSOR       391   392   RXN03080   VV0045   1670   2449   FERRIC ENTEROBACTIN TRANSPORT ATP-BINDING PROTEIN FEPC       393   394   RXN03081   VV0045   2476   2934   FERRIENTEROBACTIN-BINDING PERIPLASMIC PROTEIN PRECURSOR       395   396   RXN03082   VV0045   3131   3451   FERRIENTEROBACTIN-BINDING PERIPLASMIC PROTEIN PRECURSOR                 Other transporters                                         397   398   RXA02261   GR00654   30936   32291   AMMONIUM TRANSPORT SYSTEM       399   400   RXA02020   GR00613   1015   5   AROMATIC AMINO ACID TRANSPORT PROTEIN AROP       401   402   RXA00281   GR00043   4721   5404   BACITRACIN TRANSPORT ATP-BINDING PROTEIN BCRA       403   404   RXN00570   VV0147   855   4   BENZOATE MEMBRANE TRANSPORT PROTEIN       405   406   F RXA00570   GR00153   1   498   BENZOATE MEMBRANE TRANSPORT PROTEIN       407   408   RXN00571   VV173   1298   42   BENZOATE MEMBRANE TRANSPORT PROTEIN       409   410   F RXA00571   GR00154   2   1186   BENZOATE MEMBRANE TRANSPORT PROTEIN       411   412   RXA00962   GR00268   2   667   BENZOATE MEMBRANE TRANSPORT PROTEIN       413   414   RXA02811   GR00792   177   560   BENZOATE MEMBRANE TRANSPORT PROTEIN       415   416   RXA02115   GR00635   2   1198   BENZOATE MEMBRANE TRANSPORT PROTEIN       417   418   RXN00590   VV0178   5043   6230   BRANCHED CHAIN AMINO ACID TRANSPORT SYSTEM II                               CARRIER PROTEIN       419   420   F RXA00590   GR00157   178   564   BRANCHED CHAIN AMINO ACID TRANSPORT SYSTEM II                               CARRIER PROTEIN       421   422   F RXA01538   GR00427   5040   5429   BRANCHED CHAIN AMINO ACID TRANSPORT SYSTEM II                               CARRIER PROTEIN       423   424   RXA01727   GR00489   1471   194   BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM                               CARRIER PROTEIN       425   426   RXA00623   GR00163   6525   7862   C4-DICARBOXYLATE TRANSPORT PROTEIN       427   428   RXA01584   GR00441   55   597   CHROMATE TRANSPORT PROTEIN       429   430   RXA00852   GR00231   3137   2448   COBALT TRANSPORT ATP-BINDING PROTEIN CBIO       431   432   RXA00690   GR00181   1213   68   COBALT TRANSPORT PROTEIN CBIQ       433   434   RXA00827   GR00223   1319   567   COBALT TRANSPORT PROTEIN CBIQ       435   436   RXA00851   GR00231   2448   1840   COBALT TRANSPORT PROTEIN CBIQ       437   438   RXS03220               D-XYLOSE-PROTON SYMPORT       439   440   F RXA02762   GR00768   346   630   D-XYLOSE PROTON-SYMPORTER       441   442   RXN00092   VV0129   27509   26844   GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ       443   444   F RXA00092   GR00014   1   204   GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ       445   446   RXN03060   VV0030   6227   5376   GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ       447   448   F RXA02618   GR00745   1914   2351   GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ       449   450   F RXA02900   GR10040   2979   2128   GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ       451   452   RXS03212               GLYCINE BETAINE TRANSPORTER BETP       453   454   F RXA01591   GR00446   3   947   GLYCINE BETAINE TRANSPORTER BETP       455   456   RXN00201   VV0096   197   6   HIGH AFFINITY RIBOSE TRANSPORT PROTEIN RBSD       457   458   F RXA00201   GR00032   191   6   HIGH AFFINITY RIBOSE TRANSPORT PROTEIN RBSD       459   460   RXA01221   GR00354   2108   2833   HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID                               TRANSPORT ATP-BINDING PROTEIN BRAG       461   462   RXA01222   GR00354   2844   3542   HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID                               TRANSPORT ATP-BINDING PROTEIN LIVF       463   464   RXA01219   GR00354   151   1032   HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID                               TRANSPORT PERMEASE PROTEIN LIVH       465   466   RXA01220   GR00354   1032   2108   HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID                               TRANSPORT PERMEASE PROTEIN LIVM       467   468   RXA00091   GR00013   7762   8514   IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE       469   470   RXA00228   GR00032   29232   28642   IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE       471   472   RXA00346   GR00064   1054   1743   IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE       473   474   RXA00524   GR00135   779   1111   IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE       475   476   RXA01823   GR00516   591   1367   IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE       477   478   RXA02767   GR00770   1032   1814   IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE       479   480   RXA02792   GR00777   8581   7829   IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE       481   482   RXN02929   VV0090   36837   37874   IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD       483   484   F RXA01235   GR00358   1165   194   IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD       485   486   RXN02794   VV0134   10625   9552   IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD       487   488   F RXA01419   GR00415   888   1151   IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD       489   490   F RXA02794   GR00777   10172   9552   IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD       491   492   RXN03079   VV0045   644   1660   IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD       493   494   F RXA02865   GR10007   3832   2816   IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD       495   496   RXA00181   GR00028   3954   2383   PROLINE TRANSPORT SYSTEM       497   498   RXA00591   GR00158   229   1581   PROLINE/BETAINE TRANSPORTER       499   500   RXA01629   GR00453   3476   1965   PROLINE/BETAINE TRANSPORTER       501   502   RXA02030   GR00618   3072   1687   PROLINE/BETAINE TRANSPORTER       503   504   RXA00186   GR00028   12242   12988   SHORT-CHAIN FATTY ACIDS TRANSPORTER       505   506   RXA00187   GR00028   13097   13447   SHORT-CHAIN FATTY ACIDS TRANSPORTER       507   508   RXA01667   GR00464   703   1908   SODIUM/GLUTAMATE SYMPORT CARRIER PROTEIN       509   510   RXA02171   GR00641   6571   4919   SODIUM/PROLINE SYMPORTER       511   512   RXA00902   GR00245   4643   5875   SODIUM-DEPENDENT PHOSPHATE TRANSPORT PROTEIN       513   514   RXA00941   GR00257   1999   683   sodium-dependent phosphate transport protein       515   516   RXN00449   VV0112   30992   32572   Sodium-Dicarboxylate Symport Protein       517   518   F RXA00449   GR00109   2040   1036   Sodium-Dicarboxylate Symport Protein       519   520   F RXA01755   GR00498   352   5   Sodium-Dicarboxylate Symport Protein       521   522   RXA00269   GR00041   1826   1038   SPERMIDINE/PUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA       523   524   RXA00369   GR00076   583   1299   SPERMIDINE/PUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA       525   526   RXA02073   GR00628   4176   2647   TRANSPORT ATP-BINDING PROTEIN CYDC       527   528   RXA01399   GR00409   1   1119   TRANSPORT ATP-BINDING PROTEIN CYDD       529   530   RXA01339   GR00389   8408   7164   TYROSINE-SPECIFIC TRANSPORT PROTEIN       531   532   RXA02527   GR00725   5519   6847   2-OXOGLUTARATE/MALATE TRANSLOCATOR PRECURSOR       533   534   RXN00298   VV0176   40228   42072   HIGH-AFFINITY CHOLINE TRANSPORT PROTEIN       535   536   F RXA00298   GR00048   4459   6303   Ectoine/Proline/Glycine betaine carrier ectP       537   538   RXA00596   GR00159   335   787   potassium efflux system protein phaE       539   540   RXA02364   GR00686   841   215   C4-DICARBOXYLATE-BINDING PERIPLASMIC PROTEIN                               PRECURSOR, transport protein       541   542   RXN01411   VV0050   26015   26779   SHIKIMATE TRANSPORTER       543   544   RXN00960   VV0075   1139   105   PROTON/SODIUM-GLUTAMATE SYMPORT PROTEIN       545   546   RXN02447   VV0107   14297   13203   GALACTOSE-PROTON SYMPORT       547   548   RXN02395   VV0176   16747   14858   GLYCINE BETAINE TRANSPORTER BETP       549   550   RXN02348   VV0078   6027   7910   KUP SYSTEM POTASSIUM UPTAKE PROTEIN       551   552   RXN00297   VV0176   38630   39541   Hypothetical Malonate Transporter       553   554   RXN03103   VV0070   845   1087   GLUTAMATE-BINDING PROTEIN PRECURSOR       555   556   RXN02993   VV0071   736   65   GLUTAMINE-BINDING PROTEIN       557   558   RXN00349   VV0135   35187   36653   Hypothetical Trehalose Transport Protein       559   560   RXN03095   VV0057   4056   4424   CADMIUM EFFLUX SYSTEM ACCESSORY PROTEIN HOMOLOG       561   562   RXN03160   VV0189   5150   5617   CHROMATE TRANSPORT PROTEIN       563   564   RXN02955   VV0176   8666   9187   DICARBOXYLATE TRANSPORTER       565   566   RXN03109   VV0082   659   6   HEMIN TRANSPORT SYSTEM PERMEASE PROTEIN HMUU       567   568   RXN02979   VV0149   2150   2383   MERCURIC TRANSPORT PROTEIN PERIPLASMIC                               COMPONENT PRECURSOR       569   570   RXN02987   VV0234   527   294   MERCURIC TRANSPORT PROTEIN PERIPLASMIC                               COMPONENT PRECURSOR       571   572   RXN03084   VV0048   900   1817   IRON(III) DICITRATE-BINDING PERIPLASMIC PROTEIN PRECURSOR       573   574   RXN03183   VV0372   1   417   TREHALOSE/MALTOSE BINDING PROTEIN       575   576   RXN01139   VV0077   2776   1823   CATION EFFLUX SYSTEM PROTEIN CZCD       577   578   RXN00378   VV0223   8027   5418   Cation transport ATPases       579   580   RXN01338   VV0032   2   1903   CATION-TRANSPORTING ATPASE PACS (EC 3.6.1.—)       581   582   RXN00980   VV0149   2635   4428   CATION-TRANSPORTING P-TYPE ATPASE B (EC 3.6.1.—)       583   584   RXN00099   VV0129   18876   17704   CYANATE TRANSPORT PROTEIN CYNX       585   586   RXN02662   VV0315   1461   1724   DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPC       587   588   RXN02442   VV0217   5970   6818   zinc transport system membrane protein       589   590   RXN02443   VV0217   6818   7771   zinc-binding periplasmic protein precursor       591   592   RXN00842   VV0138   8686   7487   BRANCHED CHAIN AMINO ACID TRANSPORT SYSTEM II                               CARRIER PROTEIN       593   594   F RXA00842   GR00228   3208   2009   Permeases       595   596   RXN00832   VV0180   3133   4182   CALCIUM/PROTON ANTIPORTER       597   598   RXN00466   VV0086   63271   64266   Ferrichrome transport proteins       599   600   RXN01936   VV0127   40116   41387   MACROLIDE-EFFLUX PROTEIN       601   602   RXN01995   VV0182   2139   3476   PUTATIVE 3-(3-HYDROXYPHENYL) PROPIONATE TRANSPORT PROTEIN       603   604   RXN00661   VV0142   9718   9029   PNUC PROTEIN                 Permeases                                         605   606   RXN02566   VV0154   11823   13031   NUCLEOSIDE PERMEASE NUPG       607   608   F RXA02561   GR00732   664   5   NUCLEOSIDE PERMEASE NUPG       609   610   F RXA02566   GR00733   782   345   NUCLEOSIDE PERMEASE NUPG       611   612   RXA00051   GR00008   5770   7173   PROLINE-SPECIFIC PERMEASE PROY       613   614   RXA01172   GR00334   2687   4141   SULFATE PERMEASE       615   616   RXA02128   GR00637   2906   4600   SULFATE PERMEASE       617   618   RXA02634   GR00748   6045   7655   SULFATE PERMEASE       619   620   RXN02233   VV0068   6856   8142   URACIL PERMEASE       621   622   F RXA02233   GR00653   6856   8067   URACIL PERMEASE       623   624   RXN02372   VV0213   9311   11197   XANTHINE PERMEASE       625   626   F RXA02372   GR00688   6   560   XANTHINE PERMEASE       627   628   F RXA02377   GR00689   3336   4526   XANTHINE PERMEASE       629   630   RXA02676   GR00754   2697   1309   GLUCONATE PERMEASE       631   632   RXN00432   VV0112   14751   13267   NA(+)-LINKED D-ALANINE GLYCINE PERMEASE       633   634   F RXA00432   GR00100   1   891   NA(+)-LINKED D-ALANINE GLYCINE PERMEASE       635   636   F RXA00436   GR00101   45   569   NA(+)-LINKED D-ALANINE GLYCINE PERMEASE       637   638   RXA00847   GR00230   1829   381   OLIGOPEPTIDE-BINDING PROTEIN APPA PRECURSOR (permease)       639   640   RXN01382   VV0119   8670   9761   OLIGOPEPTIDE-BINDING PROTEIN OPPA PRECURSOR       641   642   F RXA01382   GR00405   1067   6   OLIGOPEPTIDE-BINDING PROTEIN OPPA PRECURSOR (permease)       643   644   RXA02659   GR00753   2   313   OLIGOPEPTIDE-BINDING PROTEIN OPPA PRECURSOR (permease)       645   646   RXN02933   VV0176   30042   29233   DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPC       647   648   RXN02991   VV0072   618   4   GLUTAMINE TRANSPORT SYSTEM PERMEASE PROTEIN GLNP       649   650   RXN02992   VV0072   842   621   GLUTAMINE TRANSPORT SYSTEM PERMEASE PROTEIN GLNP       651   652   RXN02996   VV0069   1980   2648   HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID                               TRANSPORT PERMEASE PROTEIN LIVH       653   654   RXN03126   VV0112   9894   9001   TEICHOIC ACID TRANSLOCATION PERMEASE PROTEIN TAGG       655   656   RXN00443   VV0112   21572   20769   MOLYBDATE-BINDING PERIPLASMIC PROTEIN PRECURSOR       657   658   RXN00444   VV0112   20785   19949   MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB       659   660   RXN00193   VV0371   1   594   POTENTIAL STARCH DEGRADATION PRODUCTS TRANSPORT SYSTEM                               PERMEASE PROTEIN AMYD       661   662   RXN01298   VV0116   2071   1142   POTENTIAL STARCH DEGRADATION PRODUCTS TRANSPORT SYSTEM                               PERMEASE PROTEIN AMYD                 Channel Proteins                                         663   664   RXA01737   GR00493   2913   3971   POTASSIUM CHANNEL PROTEIN       665   666   RXN02348   VV0078   6027   7910   KUP SYSTEM POTASSIUM UPTAKE PROTEIN       667   668   RXA02426   GR00707   2165   633   PROBABLE NA(+)/H(+) ANTIPORTER       669   670   RXN03164   VV0277   1586   2455   POTASSIUM CHANNEL BETA SUBUNIT       671   672   RXN00024   VV0127   64219   63275   POTASSIUM CHANNEL BETA SUBUNIT                 Lipoprotein and Lipopolysaccharide synthesis                                         673   674   RXN01164   VV0117   15894   14260   DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE (EC 2.4.1.83)/                               APOLIPOPROTEIN N-ACYLTRANSFERASE (EC 2.3.1.—)       675   676   RXN01168   VV0117   14224   13415   DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE (EC 2.4.1.83)/                               APOLIPOPROTEIN N-ACYLTRANSFERASE (EC 2.3.1.—)                  
 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                   
               
               
                 GENES IDENTIFIED FROM GENBANK 
               
            
           
           
               
               
               
               
            
               
                 GenBank ™ Accession No. 
                 Gene Name 
                 Gene Function 
                 Reference 
               
               
                   
               
               
                 A09073 
                 ppg 
                 Phosphoenol pyruvate carboxylase 
                 Bachmann, B. et al. “DNA fragment coding for phosphoenolpyruvat 
               
               
                   
                   
                   
                 corboxylase, recombinant DNA carrying said fragment, strains carrying the 
               
               
                   
                   
                   
                 recombinant DNA and method for producing L-aminino acids using said strains,” Patent: 
               
               
                   
                   
                   
                 EP 0358940-A 3 Mar. 21, 1990 
               
               
                 A45579, 
                   
                 Threonine dehydratase 
                 Moeckel, B. et al. “Production of L-isoleucine by means of recombinant 
               
               
                 A45581, 
                   
                   
                 micro-organisms with deregulated threonine dehydratase,” Patent: WO 
               
               
                 A45583, 
                   
                   
                 9519442-A 5 Jul. 20, 1995 
               
               
                 A45585 
               
               
                 A45587 
               
               
                 AB003132 
                 murC; ftsQ; ftsZ 
                   
                 Kobayashi, M. et al. “Cloning, sequencing, and characterization of the ftsZ 
               
               
                   
                   
                   
                 gene from  coryneform  bacteria,” Biochem. Biophys. Res. Commun., 
               
               
                   
                   
                   
                 236(2): 383-388 (1997) 
               
               
                 AB015023 
                 murC; ftsQ 
                   
                 Wachi, M. et al. “A murC gene from  Coryneform  bacteria, ” Appl. Microbiol. 
               
               
                   
                   
                   
                 Biotechnol., 51(2): 223-228 (1999) 
               
               
                 AB018530 
                 dtsR 
                   
                 Kimura, E. et al. “Molecular cloning of a novel gene, dtsR, which rescues the 
               
               
                   
                   
                   
                 detergent sensitivity of a mutant derived from  Brevibacterium   
               
               
                   
                   
                   
                   lactofermentum , ” Biosci. Biotechnol. Biochem., 60(10): 1565-1570 (1996) 
               
               
                 AB018531 
                 dtsR1; dtsR2 
               
               
                 AB020624 
                 murI 
                 D-glutamate racemase 
               
               
                 AB023377 
                 tkt 
                 transketolase 
               
               
                 AB024708 
                 gltB; gltD 
                 Glutamine 2-oxoglutarate aminotransferase 
               
               
                   
                   
                 large and small subunits 
               
               
                 AB025424 
                 acn 
                 aconitase 
               
               
                 AB027714 
                 rep 
                 Replication protein 
               
               
                 AB027715 
                 rep; aad 
                 Replication protein; aminoglycoside 
               
               
                   
                   
                 adenyltransferase 
               
               
                 AF005242 
                 argC 
                 N-acetylglutamate-5-semialdehyde 
               
               
                   
                   
                 dehydrogenase 
               
               
                 AF005635 
                 glnA 
                 Glutamine synthetase 
               
               
                 AF030405 
                 hisF 
                 cyclase 
               
               
                 AF030520 
                 argG 
                 Argininosuccinate synthetase 
               
               
                 AF031518 
                 argF 
                 Ornithine carbamolytransferase 
               
               
                 AF036932 
                 aroD 
                 3-dehydroquinate dehydratase 
               
               
                 AF038548 
                 pyc 
                 Pyruvate carboxylase 
               
               
                 AF038651 
                 dciAE; apt; rel 
                 Dipeptide-binding protein; adenine 
                 Wehmeier, L. et al. “The role of the  Corynebacterium glutamicum  rel gene in (p)ppGpp 
               
               
                   
                   
                 phosphoribosyltransferase; GTP 
                 metabolism,” Microbiology, 144: 1853-1862 (1998) 
               
               
                   
                   
                 pyrophosphokinase 
               
               
                 AF041436 
                 argR 
                 Arginine repressor 
               
               
                 AF045998 
                 impA 
                 Inositol monophosphate phosphatase 
               
               
                 AF048764 
                 argH 
                 Argininosuccinate lyase 
               
               
                 AF049897 
                 argC; argJ; argB; 
                 N-acetylglutamylphosphate reductase; 
               
               
                   
                 argD; argF; argR; 
                 ornithine acetyltransferase; N- 
               
               
                   
                 argG; argH 
                 acetylglutamate kinase; acetylornithine 
               
               
                   
                   
                 transminase; ornithine 
               
               
                   
                   
                 carbamoyltransferase; arginine repressor; 
               
               
                   
                   
                 argininosuccinate synthase; 
               
               
                   
                   
                 argininosuccinate lyase 
               
               
                 AF050109 
                 inhA 
                 Enoyl-acyl carrier protein reductase 
               
               
                 AF050166 
                 hisG 
                 ATP phosphoribosyltransferase 
               
               
                 AF051846 
                 hisA 
                 Phosphoribosylformimino-5-amino-1- 
               
               
                   
                   
                 phosphoribosyl-4-imidazolecarboxamide 
               
               
                   
                   
                 isomerase 
               
               
                 AF052652 
                 metA 
                 Homoserine O-acetyltransferase 
                 Park, S. et al. “Isolation and analysis of metA, a methionine biosynthetic gene 
               
               
                   
                   
                   
                 encoding homoserine acetyltransferase in  Corynebacterium glutamicum ,” Mol. 
               
               
                   
                   
                   
                 Cells., 8(3): 286-294 (1998) 
               
               
                 AF053071 
                 aroB 
                 Dehydroquinate synthetase 
               
               
                 AF060558 
                 hisH 
                 Glutamine amidotransferase 
               
               
                 AF086704 
                 hisE 
                 Phosphoribosyl-ATP- 
               
               
                   
                   
                 pyrophosphohydrolase 
               
               
                 AF114233 
                 aroA 
                 5-enolpyruvylshikimate 3-phosphate 
               
               
                   
                   
                 synthase 
               
               
                 AF116184 
                 panD 
                 L-aspartate-alpha-decarboxylase precursor 
                 Dusch, N. et al. “Expression of the  Corynebacterium glutamicum  panD gene 
               
               
                   
                   
                   
                 encoding L-aspartate-alpha-decarboxylase leads to pantothenate 
               
               
                   
                   
                   
                 overproduction in  Escherichia coli ,” Appl. Environ. Microbiol., 65(4)1530-1539 
               
               
                   
                   
                   
                 (1999) 
               
               
                 AF124518 
                 aroD; aroE 
                 3-dehydroquinase; shikimate 
               
               
                   
                   
                 dehydrogenase 
               
               
                 AF124600 
                 aroC; aroK; aroB; 
                 Chorismate synthase; shikimate kinase; 3- 
               
               
                   
                 pepQ 
                 dehydroquinate synthase; putative 
               
               
                   
                   
                 cytoplasmic peptidase 
               
               
                 AF145897 
                 inhA 
               
               
                 AF145898 
                 inhA 
               
               
                 AJ001436 
                 ectP 
                 Transport of ectoine, glycine betaine, 
                 Peter, H. et al. “ Corynebacterium glutamicum  is equipped with four secondary carriers 
               
               
                   
                   
                 proline 
                 for compatible solutes: Identification, sequencing, and characterization 
               
               
                   
                   
                   
                 of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine 
               
               
                   
                   
                   
                 betaine carrier, EctP,” J. Bacteriol., 180(22): 6005-6012 (1998) 
               
               
                 AJ004934 
                 dapD 
                 Tetrahydrodipicolinate succinylase 
                 Wehrmann, A. et al. “Different modes of diaminopimelate synthesis and their 
               
               
                   
                   
                 (incomplete i ) 
                 role in cell wall integrity: A study with  Corynebacterium glutamicum ,” J. 
               
               
                   
                   
                   
                 Bacteriol., 180(12): 3159-3165 (1998) 
               
               
                 AJ007732 
                 ppc; secG; amt; ocd; 
                 Phosphoenolpyruvate-carboxylase; ?; high 
               
               
                   
                 soxA 
                 affinity ammonium uptake protein; putative 
               
               
                   
                   
                 ornithine-cyclodecarboxylase; sarcosine 
               
               
                   
                   
                 oxidase 
               
               
                 AJ010319 
                 ftsY, glnB, glnD; srp; amtP 
                 Involved in cell division; PII protein; 
                 Jakoby, M. et al. “Nitrogen regulation in  Corynebacterium glutamicum  ; 
               
               
                   
                   
                 uridylyltransferase (uridylyl-removing 
                 Isolation of genes involved in biochemical characterization of corresponding 
               
               
                   
                   
                 enzmye); signal recognition particle; low 
                 proteins,” FEMS Microbiol., 173(2): 303-310 (1999) 
               
               
                   
                   
                 affinity ammonium uptake protein 
               
               
                 AJ132968 
                 cat 
                 Chloramphenicol aceteyl transferase 
               
               
                 AJ224946 
                 mqo 
                 L-malate: quinone oxidoreductase 
                 Molenaar, D. et al. “Biochemical and genetic characterization of the 
               
               
                   
                   
                   
                 membrane-associated malate dehydrogenase (acceptor) from  Corynebacterium   
               
               
                   
                   
                   
                   glutamicum ,” Eur. J. Biochem., 254(2): 395-403 (1998) 
               
               
                 AJ238250 
                 ndh 
                 NADH dehydrogenase 
               
               
                 AJ238703 
                 porA 
                 Porin 
                 Lichtinger, T. et al. “Biochemical and biophysical characterization of the cell 
               
               
                   
                   
                   
                 wall porin of  Corynebacterium glutamicum : The channel is formed by a low 
               
               
                   
                   
                   
                 molecular mass polypeptide,” Biochemistry, 37(43): 15024-15032 (1998) 
               
               
                 D17429 
                   
                 Transposable element IS31831 
                 Vertes, A. A. et al. “Isolation and characterization of IS31831, a transposable 
               
               
                   
                   
                   
                 element from  Corynebacterium glutamicum ,” Mol. Microbiol., 11(4): 739-746 
               
               
                   
                   
                   
                 (1994) 
               
               
                 D84102 
                 odhA 
                 2-oxoglutarate dehydrogenase 
                 Usuda, Y. et al. “Molecular cloning of the  Corynebacterium glutamicum    
               
               
                   
                   
                   
                 ( Brevibacterium lactofermentum  AJ12036) odhA gene encoding a novel type 
               
               
                   
                   
                   
                 of 2-oxoglutarate dehydrogenase,” Microbiology, 142: 3347-3354 (1996) 
               
               
                 E01358 
                 hdh; hk 
                 Homoserine dehydrogenase; homoserine 
                 Katsumata, R. et al. “Production of L-thereonine and L-isoleucine,” Patent: JP 
               
               
                   
                   
                 kinase 
                 1987232392-A 1 Oct. 12, 1987 
               
               
                 E01359 
                   
                 Upstream of the start codon of homoserine 
                 Katsumata, R. et al. “Production of L-thereonine and L-isoleucine,” Patent: JP 
               
               
                   
                   
                 kinase gene 
                 1987232392-A 2 Oct. 12, 1987 
               
               
                 E01375 
                   
                 Tryptophan operon 
               
               
                 E01376 
                 trpL; trpE 
                 Leader peptide; anthranilate synthase 
                 Matsui, K. et al. “Tryptophan operon, peptide and protein coded thereby, 
               
               
                   
                   
                   
                 utilization of tryptophan operon gene expression and production of 
               
               
                   
                   
                   
                 tryptophan,” Patent: JP 1987244382-A 1 Oct. 24, 1987 
               
               
                 E01377 
                   
                 Promoter and operator regions of 
                 Matsui, K. et al. “Tryptophan operon, peptide and protein coded thereby, 
               
               
                   
                   
                 tryptophan operon 
                 utilization of tryptophan operon gene expression and production of 
               
               
                   
                   
                   
                 tryptophan,” Patent: JP 1987244382-A 1 Oct. 24, 1987 
               
               
                 E03937 
                   
                 Biotin-synthase 
                 Hatakeyama, K. et al. “DNA fragment containing gene capable of coding 
               
               
                   
                   
                   
                 biotin synthetase and its utilization,” Patent: JP 1992278088-A 1 Oct. 02, 1992 
               
               
                 E04040 
                   
                 Diamino pelargonic acid aminotransferase 
                 Kohama, K. et al. “Gene coding diaminopelargonic acid aminotransferase and 
               
               
                   
                   
                   
                 desthiobiotin synthetase and its utilization,” Patent: JP 1992330284-A 1 
               
               
                   
                   
                   
                 Nov. 18, 1992 
               
               
                 E04041 
                   
                 Desthiobiotinsynthetase 
                 Kohama, K. et al. “Gene coding diaminopelargonic acid aminotransferase and 
               
               
                   
                   
                   
                 desthiobiotin synthetase and its utilization,” Patent: JP 1992330284-A 1 
               
               
                   
                   
                   
                 Nov. 18, 1992 
               
               
                 E04307 
                   
                 Flavum aspartase 
                 Kurusu, Y. et al. “Gene DNA coding aspartase and utilization thereof,” Patent: 
               
               
                   
                   
                   
                 JP 1993030977-A 1 Feb. 09, 1993 
               
               
                 E04376 
                   
                 Isocitric acid lyase 
                 Katsumata, R. et al. “Gene manifestation controlling DNA,” Patent: JP 
               
               
                   
                   
                   
                 1993056782-A 3 Mar. 09, 1993 
               
               
                 A09073 
                 ppg 
                 Phosphoenol pyruvate carboxylase 
                 Bachmann, B. et al. “DNA fragment coding for phosphoenolpyruvat 
               
               
                 E04377 
                   
                 Isocitric acid lyase N-terminal fragment 
                 Katsumata, R. et al. “Gene manifestation controlling DNA,” Patent: JP 
               
               
                   
                   
                   
                 1993056782-A 3 Mar. 09, 1993 
               
               
                 E04484 
                   
                 Prephenate dehydratase 
                 Sotouchi, N. et al. “Production of L-phenylalanine by fermentation,” Patent: JP 
               
               
                   
                   
                   
                 1993076352-A 2 Mar. 30, 1993 
               
               
                 E05108 
                   
                 Aspartokinase 
                 Fugono, N. et al. “Gene DNA coding Aspartokinase and its use,” Patent: JP 
               
               
                   
                   
                   
                 1993184366-A 1 Jul. 27, 1993 
               
               
                 E05112 
                   
                 Dihydro-dipichorinate synthetase 
                 Hatakeyama, K. et al. “Gene DNA coding dihydrodipicolinic acid synthetase 
               
               
                   
                   
                   
                 and its use,” Patent: JP 1993184371-A 1 Jul. 27, 1993 
               
               
                 E05776 
                   
                 Diaminopimelic acid dehydrogenase 
                 Kobayashi, M. et al. “Gene DNA coding Diaminopimelic acid dehydrogenase 
               
               
                   
                   
                   
                 and its use,” Patent: JP 1993284970-A 1 Nov. 02, 1993 
               
               
                 E05779 
                   
                 Threonine synthase 
                 Kohama, K. et al. “Gene DNA coding threonine synthase and its use,” Patent: 
               
               
                   
                   
                   
                 JP 1993284972-A 1 Nov. 02, 1993 
               
               
                 E06110 
                   
                 Prephenate dehydratase 
                 Kikuchi, T. et al. “Production of L-phenylalanine by fermentation method,” 
               
               
                   
                   
                   
                 Patent: JP 1993344881-A 1 Dec. 27, 1993 
               
               
                 E06111 
                   
                 Mutated Prephenate dehydratase 
                 Kikuchi, T. et al. “Production of L-phenylalanine by fermentation method,” 
               
               
                   
                   
                   
                 Patent: JP 1993344881-A 1 Dec. 27, 1993 
               
               
                 E06146 
                   
                 Acetohydroxy acid synthetase 
                 Inui, M. et al. “Gene capable of coding Acetohydroxy acid synthetase and its 
               
               
                   
                   
                   
                 use,” Patent: JP 1993344893-A 1 Dec. 27, 1993 
               
               
                 E06825 
                   
                 Aspartokinase 
                 Sugimoto, M. et al. “Mutant aspartokinase gene,” patent: JP 1994062866-A 1 
               
               
                   
                   
                   
                 Mar. 08, 1994 
               
               
                 E06826 
                   
                 Mutated aspartokinase alpha subunit 
                 Sugimoto, M. et al. “Mutant aspartokinase gene,” patent: JP 1994062866-A 1 
               
               
                   
                   
                   
                 Mar. 08, 1994 
               
               
                 E06827 
                   
                 Mutated aspartokinase alpha subunit 
                 Sugimoto, M. et al. “Mutant aspartokinase gene,” patent: JP 1994062866-A 1 
               
               
                   
                   
                   
                 Mar. 08, 1994 
               
               
                 E07701 
                 secY 
                   
                 Honno, N. et al. “Gene DNA participating in integration of membraneous 
               
               
                   
                   
                   
                 protein to membrane,” Patent: JP 1994169780-A 1 Jun. 21, 1994 
               
               
                 E08177 
                   
                 Aspartokinase 
                 Sato, Y. et al. “Genetic DNA capable of coding Aspartokinase released from 
               
               
                   
                   
                   
                 feedback inhibition and its utilization,” Patent: JP 1994261766-A 1 Sep. 20, 1994 
               
               
                 E08178, 
                   
                 Feedback inhibition-released Aspartokinase 
                 Sato, Y. et al. “Genetic DNA capable of coding Aspartokinase released from 
               
               
                 E08179, 
                   
                   
                 feedback inhibition and its utilization,” Patent: JP 1994261766-A 1 Sep. 20, 1994 
               
               
                 E08180, 
               
               
                 E08181, 
               
               
                 E08182 
               
               
                 E08232 
                   
                 Acetohydroxy-acid isomeroreductase 
                 Inui, M. et al. “Gene DNA coding acetohydroxy acid isomeroreductase,” 
               
               
                   
                   
                   
                 Patent: JP 1994277067-A 1 Oct. 04, 1994 
               
               
                 E08234 
                 secE 
                   
                 Asai, Y. et al. “Gene DNA coding for translocation machinery of protein,” 
               
               
                   
                   
                   
                 Patent: JP 1994277073-A 1 Oct. 04, 1994 
               
               
                 E08643 
                   
                 FT aminotransferase and desthiobiotin 
                 Hatakeyama, K. et al. “DNA fragment having promoter function in 
               
               
                   
                   
                 synthetase promoter region 
                   coryneform bacterium ,” Patent: JP 1995031476-A 1 Feb. 03, 1995 
               
               
                 E08646 
                   
                 Biotin synthetase 
                 Hatakeyama, K. et al. “DNA fragment having promoter function in 
               
               
                   
                   
                   
                   coryneform bacterium ,” Patent: JP 1995031476-A 1 Feb. 03, 1995 
               
               
                 E08649 
                   
                 Aspartase 
                 Kohama, K. et al “DNA fragment having promoter function in  coryneform   
               
               
                   
                   
                   
                   bacterium ,” Patent: JP 1995031478-A 1 Feb. 03, 1995 
               
               
                 E08900 
                   
                 Dihydrodipicolinate reductase 
                 Madori, M. et al. “DNA fragment containing gene coding Dihydrodipicolinate 
               
               
                   
                   
                   
                 acid reductase and utilization thereof,” Patent: JP 1995075578-A 1 Mar. 20, 1995 
               
               
                 E08901 
                   
                 Diaminopimelic acid decarboxylase 
                 Madori, M. et al. “DNA fragment containing gene coding Diaminopimelic acid 
               
               
                   
                   
                   
                 decarboxylase and utilization thereof,” Patent: JP 1995075579-A 1 Mar. 20, 1995 
               
               
                 E12594 
                   
                 Serine hydroxymethyltransferase 
                 Hatakeyama, K. et al. “Production of L-trypophan,” Patent: JP 1997028391-A 
               
               
                   
                   
                   
                 1 Feb. 4, 1997 
               
               
                 E12760, 
                   
                 transposase 
                 Moriya, M. et al. “Amplification of gene using artificial transposon,” Patent: 
               
               
                 E12759, 
                   
                   
                 JP 1997070291-A Mar. 18, 1997 
               
               
                 E12758 
               
               
                 E12764 
                   
                 Arginyl-tRNA synthetase; diaminopimelic 
                 Moriya, M. et al. “Amplification of gene using artificial transposon,” Patent: 
               
               
                   
                   
                 acid decarboxylase 
                 JP 1997070291-A Mar. 18, 1997 
               
               
                 E12767 
                   
                 Dihydrodipicolinic acid synthetase 
                 Moriya, M. et al. “Amplification of gene using artificial transposon,” Patent: 
               
               
                   
                   
                   
                 JP 1997070291-A Mar. 18, 1997 
               
               
                 E12770 
                   
                 aspartokinase 
                 Moriya, M. et al. “Amplification of gene using artificial transposon,” Patent: 
               
               
                   
                   
                   
                 JP 1997070291-A Mar. 18, 1997 
               
               
                 E12773 
                   
                 Dihydrodipicolinic acid reductase 
                 Moriya, M. et al. “Amplification of gene using artificial transposon,” Patent: 
               
               
                   
                   
                   
                 JP 1997070291-A Mar. 18, 1997 
               
               
                 E13655 
                   
                 Glucose-6-phosphate dehydrogenase 
                 Hatakeyama, K. et al. “Glucose-6-phosphate dehydrogenase and DNA capable 
               
               
                   
                   
                   
                 of coding the same,” Patent: JP 1997224661-A 1 Sep. 02, 1997 
               
               
                 L01508 
                 IlvA 
                 Threonine dehydratase 
                 Moeckel, B. et al. “Functional and structural analysis of the threonine 
               
               
                   
                   
                   
                 dehydratase of  Corynebacterium glutamicum ,” J. Bacteriol., 174: 8065-8072 
               
               
                   
                   
                   
                 (1992) 
               
               
                 L07603 
                 EC 4.2.1.15 
                 3-deoxy-D-arabinoheptulosonate-7- 
                 Chen, C. et al. “The cloning and nucleotide sequence of  Corynebacterium   
               
               
                   
                   
                 phosphate synthase 
                   glutamicum  3-deoxy-D-arabinoheptulosonate-7-phosphate synthase gene,” 
               
               
                   
                   
                   
                 FEMS Microbiol. Lett., 107: 223-230 (1993) 
               
               
                 L09232 
                 IlvB; ilvN; ilvC 
                 Acetohydroxy acid synthase large subunit; 
                 Keilhauer, C. et al. “Isoleucine synthesis in  Corynebacterium glutamicum : 
               
               
                   
                   
                 Acetohydroxy acid synthase small subunit; 
                 molecular analysis of the ilvB-ilvN-ilvC operon,” J. Bacteriol., 175(17): 5595-5603 
               
               
                   
                   
                 Acetohydroxy acid isomeroreductase 
                 (1993) 
               
               
                 L18874 
                 PtsM 
                 Phosphoenolpyruvate sugar 
                 Fouet, A et al. “ Bacillus subtilis  sucrose-specific enzyme II of the 
               
               
                   
                   
                 phosphotransferase 
                 phosphotransferase system: expression in  Escherichia coli  and homology to 
               
               
                   
                   
                   
                 enzymes II from enteric bacteria,” PNAS USA, 84(24): 8773-8777 (1987); Lee, J. K. 
               
               
                   
                   
                   
                 et al. “Nucleotide sequence of the gene encoding the  Corynebacterium   
               
               
                   
                   
                   
                 glutamicum mannose enzyme II and analyses of the deduced protein 
               
               
                   
                   
                   
                 sequence,” FEMS Microbiol. Lett., 119(1-2): 137-145 (1994) 
               
               
                 L27123 
                 aceB 
                 Malate synthase 
                 Lee, H-S. et al. “Molecular characterization of aceB, a gene encoding malate 
               
               
                   
                   
                   
                 synthase in  Corynebacterium glutamicum ,” J. Microbiol. Biotechnol., 
               
               
                   
                   
                   
                 4(4): 256-263 (1994) 
               
               
                 L27126 
                   
                 Pyruvate kinase 
                 Jetten, M. S. et al. “Structural and functional analysis of pyruvate kinase from 
               
               
                   
                   
                   
                   Corynebacterium glutamicum ,” Appl. Environ. Microbiol., 60(7): 2501-2507 
               
               
                   
                   
                   
                 (1994) 
               
               
                 L28760 
                 aceA 
                 Isocitrate lyase 
               
               
                 L35906 
                 dtxr 
                 Diphtheria toxin repressor 
                 Oguiza, J. A. et al. “Molecular cloning, DNA sequence analysis, and 
               
               
                   
                   
                   
                 characterization of the  Corynebacterium diphtheriae  dtxR from  Brevibacterium   
               
               
                   
                   
                   
                   lactofermentum ,” J. Bacteriol., 177(2): 465-467 (1995) 
               
               
                 M13774 
                   
                 Prephenate dehydratase 
                 Follettie, M. T. et al. “Molecular cloning and nucleotide sequence of the 
               
               
                   
                   
                   
                   Corynebacterium glutamicum  pheA gene,” J. Bacteriol., 167: 695-702 (1986) 
               
               
                 M16175 
                 5S rRNA 
                   
                 Park, Y-H. et al. “Phylogenetic analysis of the  coryneform  bacteria by 56 
               
               
                   
                   
                   
                 rRNA sequences,” J. Bacteriol., 169: 1801-1806 (1987) 
               
               
                 M16663 
                 trpE 
                 Anthranilate synthase, 5′ end 
                 Sano, K. et al. “Structure and function of the trp operon control regions of 
               
               
                   
                   
                   
                   Brevibacterium lactofermentum  , a glutamic-acid-producing bacterium,” Gene, 
               
               
                   
                   
                   
                 52: 191-200 (1987) 
               
               
                 M16664 
                 trpA 
                 Tryptophan synthase, 3′end 
                 Sano, K. et al. “Structure and function of the trp operon control regions of 
               
               
                   
                   
                   
                   Brevibacterium lactofermentum  , a glutamic-acid-producing bacterium,” Gene, 
               
               
                   
                   
                   
                 52: 191-200 (1987) 
               
               
                 M25819 
                   
                 Phosphoenolpyruvate carboxylase 
                 O&#39;Regan, M. et al. “Cloning and nucleotide sequence of the 
               
               
                   
                   
                   
                 Phosphoenolpyruvate carboxylase-coding gene of  Corynebacterium   
               
               
                   
                   
                   
                   glutamicum  ATCC13032,” Gene, 77(2): 237-251 (1989) 
               
               
                 M85106 
                   
                 23S rRNA gene insertion sequence 
                 Roller, C. et al. “Gram-positive bacteria with a high DNA G + C content are 
               
               
                   
                   
                   
                 characterized by a common insertion within their 23S rRNA genes,” J. Gen. 
               
               
                   
                   
                   
                 Microbiol., 138: 1167-1175 (1992) 
               
               
                 M85107, 
                   
                 23S rRNA gene insertion sequence 
                 Roller, C. et al. “Gram-positive bacteria with a high DNA G + C content are 
               
               
                 M85108 
                   
                   
                 characterized by a common insertion within their 23S rRNA genes,” J. Gen. 
               
               
                   
                   
                   
                 Microbiol., 138: 1167-1175 (1992) 
               
               
                 M89931 
                 aecD; brnQ; yhbw 
                 Beta C-S lyase; branched-chain amino acid 
                 Rossol, I. et al. “The  Corynebacterium glutamicum  aecD gene encodes a C-S 
               
               
                   
                   
                 uptake carrier; hypothetical protein yhbw 
                 lyase with alpha, beta-elimination activity that degrades aminoethylcysteine,” 
               
               
                   
                   
                   
                 J. Bacteriol., 174(9): 2968-2977 (1992); Tauch, A. et al. “Isoleucine uptake in 
               
               
                   
                   
                   
                   Corynebacterium glutamicum  ATCC 13032 is directed by the brnQ gene 
               
               
                   
                   
                   
                 product,” Arch. Microbiol., 169(4): 303-312 (1998) 
               
               
                 S59299 
                 trp 
                 Leader gene (promoter) 
                 Herry, D. M. et al. “Cloning of the trp gene cluster from a tryptophan- 
               
               
                   
                   
                   
                 hyperproducing strain of  Corynebacterium glutamicum : identification of a 
               
               
                   
                   
                   
                 mutation in the trp leader sequence,” Appl. Environ. Microbiol., 59(3): 791-799 
               
               
                   
                   
                   
                 (1993) 
               
               
                 U11545 
                 trpD 
                 Anthranilate phosphoribosyltransferase 
                 O&#39;Gara, J. P. and Dunican, L. K. (1994) Complete nucleotide sequence of the 
               
               
                   
                   
                   
                   Corynebacterium glutamicum  ATCC 21850 tpD gene.” Thesis, Microbiology 
               
               
                   
                   
                   
                 Department, University College Galway, Ireland. 
               
               
                 U13922 
                 cglIM; cglIR; clgIIR 
                 Putative type II 5-cytosoine 
                 Schafer, A. et al. “Cloning and characterization of a DNA region encoding a 
               
               
                   
                   
                 methyltransferase; putative type II 
                 stress-sensitive restriction system from  Corynebacterium glutamicum  ATCC 
               
               
                   
                   
                 restriction endonuclease; putative type I or 
                 13032 and analysis of its role in intergeneric conjugation with  Escherichia   
               
               
                   
                   
                 type III restriction endonuclease 
                   coli ,” J. Bacteriol., 176(23): 7309-7319 (1994); Schafer, A. et al. “The 
               
               
                   
                   
                   
                   Corynebacterium glutamicum  cglIM gene encoding a 5-cytosine in an McrBC- 
               
               
                   
                   
                   
                 deficient  Escherichia coli  strain,” Gene, 203(2): 95-101 (1997) 
               
               
                 U14965 
                 recA 
               
               
                 U31224 
                 ppx 
                   
                 Ankri, S. et al. “Mutations in the  Corynebacterium glutamicum  proline 
               
               
                   
                   
                   
                 biosynthetic pathway: A natural bypass of the proA step,” J. Bacteriol., 
               
               
                   
                   
                   
                 178(15): 4412-4419 (1996) 
               
               
                 U31225 
                 proC 
                 L-proline: NADP+ 5-oxidoreductase 
                 Ankri, S. et al. “Mutations in the  Corynebacterium glutamicum  proline 
               
               
                   
                   
                   
                 biosynthetic pathway: A natural bypass of the proA step,” J. Bacteriol., 
               
               
                   
                   
                   
                 178(15): 4412-4419 (1996) 
               
               
                 U31230 
                 obg; proB; unkdh 
                 ?; gamma glutamyl kinase; similar to D- 
                 Ankri, S. et al. “Mutations in the  Corynebacterium glutamicum  proline 
               
               
                   
                   
                 isomer specific 2-hydroxyacid 
                 biosynthetic pathway: A natural bypass of the proA step,” J. Bacteriol., 
               
               
                   
                   
                 dehydrogenases 
                 178(15): 4412-4419 (1996) 
               
               
                 U31281 
                 bioB 
                 Biotin synthase 
                 Serebriiskii, I. G., “Two new members of the bio B superfamily: Cloning, 
               
               
                   
                   
                   
                 sequencing and expression of bio B genes of Methylobacillus flagellatum and 
               
               
                   
                   
                   
                   Corynebacterium glutamicum ,” Gene, 175: 15-22 (1996) 
               
               
                 U35023 
                 thtR; accBC 
                 Thiosulfate sulfurtransferase; acyl CoA 
                 Jager, W. et al. “A  Corynebacterium glutamicum  gene encoding a two-domain 
               
               
                   
                   
                 carboxylase 
                 protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins,” 
               
               
                   
                   
                   
                 Arch. Microbiol., 166(2); 76-82 (1996) 
               
               
                 U43535 
                 cmr 
                 Multidrug resistance protein 
                 Jager, W. et al. “A  Corynebacterium glutamicum  gene conferring multidrug 
               
               
                   
                   
                   
                 resistance in the heterologous host  Escherichia coli ,” J. Bacteriol., 
               
               
                   
                   
                   
                 179(7): 2449-2451 (1997) 
               
               
                 U43536 
                 clpB 
                 Heat shock ATP-binding protein 
               
               
                 U53587 
                 aphA-3 
                 3′5″-aminoglycoside phosphotransferase 
               
               
                 U89648 
                   
                   Corynebacterium glutamicum  unidentified 
               
               
                   
                   
                 sequence involved in histidine biosynthesis, 
               
               
                   
                   
                 partial sequence 
               
               
                 X04960 
                 trpA; trpB; trpC; trpD; trpE; trpG; trpL 
                 Tryptophan operon 
                 Matsui, K. et al. “Complete nucleotide and deduced amino acid sequences of 
               
               
                   
                   
                   
                 the  Brevibacterium lactofermentum  tryptophan operon,” Nucleic Acids Res., 14(24): 
               
               
                   
                   
                   
                 10113-10114 (1986) 
               
               
                 X07563 
                 lys A 
                 DAP decarboxylase (meso-diaminopimelate 
                 Yeh, P. et al. “Nucleic sequence of the lysA gene of  Corynebacterium   
               
               
                   
                   
                 decarboxylase, EC 4.1.1.20) 
                   glutamicum  and possible mechanisms for modulation of its expression,” Mol. 
               
               
                   
                   
                   
                 Gen. Genet., 212(1): 112-119 (1988) 
               
               
                 X14234 
                 EC 4.1.1.31 
                 Phosphoenolpyruvate carboxylase 
                 Eikmanns, B. J. et al. “The Phosphoenolpyruvate carboxylase gene of 
               
               
                   
                   
                   
                   Corynebacterium glutamicum : Molecular cloning, nucleotide sequence, and 
               
               
                   
                   
                   
                 expression,” Mol. Gen. Genet., 218(2): 330-339 (1989); Lepiniec, L. et al. 
               
               
                   
                   
                   
                 “Sorghum Phosphoenolpyruvate carboxylase gene family: structure, function 
               
               
                   
                   
                   
                 and molecular evolution,” Plant. Mol. Biol., 21 (3): 487-502 (1993) 
               
               
                 X17313 
                 fda 
                 Fructose-bisphosphate aldolase 
                 Von der Osten, C. H. et al. “Molecular cloning, nucleotide sequence and fine- 
               
               
                   
                   
                   
                 structural analysis of the  Corynebacterium glutamicum  fda gene: structural 
               
               
                   
                   
                   
                 comparison of  C. glutamicum  fructose-1,6-biphosphate aldolase to class I and 
               
               
                   
                   
                   
                 class II aldolases,” Mol. Microbiol., 
               
               
                 X53993 
                 dapA 
                 L-2,3-dihydrodipicolinate synthetase (EC 
                 Bonnassie, S. et al. “Nucleic sequence of the dapA gene from 
               
               
                   
                   
                 4.2.1.52) 
                   Corynebacterium glutamicum ,” Nucleic Acids Res., 18(21): 6421 (1990) 
               
               
                 X54223 
                   
                 AttB-related site 
                 Cianciotto, N. et al. “DNA sequence homology between att B-related sites of 
               
               
                   
                   
                   
                   Corynebacterium diphtheriae ,  Corynebacterium ulcerans ,  Corynebacterium   
               
               
                   
                   
                   
                   glutamicum , and the attP site of lambdacorynephage,” FEMS. Microbiol, 
               
               
                   
                   
                   
                 Lett., 66: 299-302 (1990) 
               
               
                 X54740 
                 argS; lysA 
                 Arginyl-tRNA synthetase; Diaminopimelate 
                 Marcel, T. et al. “Nucleotide sequence and organization of the upstream region 
               
               
                   
                   
                 decarboxylase 
                 of the  Corynebacterium glutamicum  lysA gene,” Mol. Microbiol., 4(11): 1819-1830 
               
               
                   
                   
                   
                 (1990) 
               
               
                 X55994 
                 trpL; trpE 
                 Putative leader peptide; anthranilate 
                 Heery, D. M. et al. “Nucleotide sequence of the  Corynebacterium glutamicum    
               
               
                   
                   
                 synthase component 1 
                 trpE gene,” Nucleic Acids Res., 18(23): 7138 (1990) 
               
               
                 X56037 
                 thrC 
                 Threonine synthase 
                 Han, K. S. et al. “The molecular structure of the  Corynebacterium glutamicum    
               
               
                   
                   
                   
                 threonine synthase gene,” Mol. Microbiol., 4(10): 1693-1702 (1990) 
               
               
                 X56075 
                 attB-related site 
                 Attachment site 
                 Cianciotto, N. et al. “DNA sequence homology between att B-related sites of 
               
               
                   
                   
                   
                   Corynebacterium diphtheriae ,  Corynebacterium ulcerans  ,  Corynebacterium   
               
               
                   
                   
                   
                   glutamicum , and the attP site of lambdacorynephage,” FEMS. Microbiol, 
               
               
                   
                   
                   
                 Lett., 66: 299-302 (1990) 
               
               
                 X57226 
                 lysC-alpha; lysC-beta; 
                 Aspartokinase-alpha subunit; 
                 Kalinowski, J. et al. “Genetic and biochemical analysis of the Aspartokinase 
               
               
                   
                 asd 
                 Aspartokinase-beta subunit; aspartate beta 
                 from  Corynebacterium glutamicum ,” Mol. Microbiol., 5(5): 1197-1204 (1991); 
               
               
                   
                   
                 semialdehyde dehydrogenase 
                 Kalinowski, J. et al. “Aspartokinase genes lysC alpha and lysC beta overlap 
               
               
                   
                   
                   
                 and are adjacent to the aspertate beta-semialdehyde dehydrogenase gene asd in 
               
               
                   
                   
                   
                   Corynebacterium glutamicum ,” Mol. Gen. Genet., 224(3): 317-324 (1990) 
               
               
                 X59403 
                 gap; pgk; tpi 
                 Glyceraldehyde-3-phosphate; 
                 Eikmanns, B. J. “Identification, sequence analysis, and expression of a 
               
               
                   
                   
                 phosphoglycerate kinase; triosephosphate 
                   Corynebacterium glutamicum  gene cluster encoding the three glycolytic 
               
               
                   
                   
                 isomerase 
                 enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate 
               
               
                   
                   
                   
                 kinase, and triosephosphate isomeras,” J. Bacteriol., 174(19): 6076-6086 
               
               
                   
                   
                   
                 (1992) 
               
               
                 X59404 
                 gdh 
                 Glutamate dehydrogenase 
                 Bormann, E. R. et al. “Molecular analysis of the  Corynebacterium glutamicum    
               
               
                   
                   
                   
                 gdh gene encoding glutamate dehydrogenase,” Mol. Microbiol., 6(3): 317-326 
               
               
                   
                   
                   
                 (1992) 
               
               
                 X60312 
                 lysI 
                 L-lysine permease 
                 Seep-Feldhaus, A. H. et al. “Molecular analysis of the  Corynebacterium   
               
               
                   
                   
                   
                   glutamicum  lysI gene involved in lysine uptake,” Mol. Microbiol., 5(12): 2995-3005 
               
               
                   
                   
                   
                 (1991) 
               
               
                 X66078 
                 cop1 
                 Ps1 protein 
                 Joliff, G. et al. “Cloning and nucleotide sequence of the csp1 gene encoding 
               
               
                   
                   
                   
                 PS1, one of the two major secreted proteins of  Corynebacterium glutamicum : 
               
               
                   
                   
                   
                 The deduced N-terminal region of PS1 is similar to the Mycobacterium antigen 
               
               
                   
                   
                   
                 85 complex,” Mol. Microbiol., 6(16): 2349-2362 (1992) 
               
               
                 X66112 
                 glt 
                 Citrate synthase 
                 Eikmanns, B. J. et al. “Cloning sequence, expression and transcriptional 
               
               
                   
                   
                   
                 analysis of the  Corynebacterium glutamicum  gltA gene encoding citrate 
               
               
                   
                   
                   
                 synthase,” Microbiol., 140: 1817-1828 (1994) 
               
               
                 X67737 
                 dapB 
                 Dihydrodipicolinate reductase 
               
               
                 X69103 
                 csp2 
                 Surface layer protein PS2 
                 Peyret, J. L. et al. “Characterization of the cspB gene encoding PS2, an ordered 
               
               
                   
                   
                   
                 surface-layer protein in  Corynebacterium glutamicum ,” Mol. Microbiol., 
               
               
                   
                   
                   
                 9(1): 97-109 (1993) 
               
               
                 X69104 
                   
                 IS3 related insertion element 
                 Bonamy, C. et al. “Identification of IS1206, a  Corynebacterium glutamicum    
               
               
                   
                   
                   
                 IS3-related insertion sequence and phylogenetic analysis,” Mol. Microbiol., 
               
               
                   
                   
                   
                 14(3): 571-581 (1994) 
               
               
                 X70959 
                 leuA 
                 Isopropylmalate synthase 
                 Patek, M. et al. “Leucine synthesis in  Corynebacterium glutamicum : enzyme 
               
               
                   
                   
                   
                 activities, structure of leuA, and effect of leuA inactivation on lysine 
               
               
                   
                   
                   
                 synthesis,” Appl. Environ. Microbiol., 60(1): 133-140 (1994) 
               
               
                 X71489 
                 icd 
                 Isocitrate dehydrogenase (NADP+) 
                 Eikmanns, B. J. et al. “Cloning sequence analysis, expression, and inactivation 
               
               
                   
                   
                   
                 of the  Corynebacterium glutamicum  icd gene encoding isocitrate 
               
               
                   
                   
                   
                 dehydrogenase and biochemical characterization of the enzyme,” J. Bacteriol., 
               
               
                   
                   
                   
                 177(3): 774-782 (1995) 
               
               
                 X72855 
                 GDHA 
                 Glutamate dehydrogenase (NADP+) 
               
               
                 X75083, 
                 mtrA 
                 5-methyltryptophan resistance 
                 Heery, D. M. et al. “A sequence from a tryptophan-hyperproducing strain of 
               
               
                 X70584 
                   
                   
                   Corynebacterium glutamicum  encoding resistance to 5-methyltryptophan,” 
               
               
                   
                   
                   
                 Biochem. Biophys. Res. Commun., 201(3): 1255-1262 (1994) 
               
               
                 X75085 
                 recA 
                   
                 Fitzpatrick, R. et al. “Construction and characterization of recA mutant strains 
               
               
                   
                   
                   
                 of  Corynebacterium glutamicum  and  Brevibacterium lactofermentum ,” Appl. 
               
               
                   
                   
                   
                 Microbiol. Biotechnol., 42(4): 575-580 (1994) 
               
               
                 X75504 
                 aceA; thiX 
                 Partial Isocitrate lyase; ? 
                 Reinscheid, D. J. et al. “Characterization of the isocitrate lyase gene from 
               
               
                   
                   
                   
                   Corynebacterium glutamicum  and biochemical analysis of the enzyme,” J. 
               
               
                   
                   
                   
                 Bacteriol., 176(12): 3474-3483 (1994) 
               
               
                 X76875 
                   
                 ATPase beta-subunit 
                 Ludwig, W. et al. “Phylogenetic relationships of bacteria based on comparative 
               
               
                   
                   
                   
                 sequence analysis of elongation factor Tu and ATP-synthase beta-subunit 
               
               
                   
                   
                   
                 genes,” Antonie Van Leeuwenhoek, 64: 285-305 (1993) 
               
               
                 X77034 
                 tuf 
                 Elongation factor Tu 
                 Ludwig, W. et al. “Phylogenetic relationships of bacteria based on comparative 
               
               
                   
                   
                   
                 sequence analysis of elongation factor Tu and ATP-synthase beta-subunit 
               
               
                   
                   
                   
                 genes,” Antonie Van Leeuwenhoek, 64: 285-305 (1993) 
               
               
                 X77384 
                 recA 
                   
                 Billman-Jacobe, H. “Nucleotide sequence of a recA gene from 
               
               
                   
                   
                   
                   Corynebacterium glutamicum ,” DNA Seq., 4(6): 403-404 (1994) 
               
               
                 X78491 
                 aceB 
                 Malate synthase 
                 Reinscheid, D. J. et al. “Malate synthase from  Corynebacterium glutamicum    
               
               
                   
                   
                   
                 pta-ack operon encoding phosphotransacetylase: sequence analysis,” 
               
               
                   
                   
                   
                 Microbiology, 140: 3099-3108 (1994) 
               
               
                 X80629 
                 16S rDNA 
                 16S ribosomal RNA 
                 Rainey, F. A. et al. “Phylogenetic analysis of the genera Rhodococcus and 
               
               
                   
                   
                   
                 Norcardia and evidence for the evolutionary origin of the genus Norcardia 
               
               
                   
                   
                   
                 from within the radiation of Rhodococcus species,” Microbiol., 141: 523-528 
               
               
                   
                   
                   
                 (1995) 
               
               
                 X81191 
                 gluA; gluB; gluC; 
                 Glutamate uptake system 
                 Kronemeyer, W. et al. “Structure of the gluABCD cluster encoding the 
               
               
                   
                 gluD 
                   
                 glutamate uptake system of  Corynebacterium glutamicum ,” J. Bacteriol., 
               
               
                   
                   
                   
                 177(5): 1152-1158 (1995) 
               
               
                 X81379 
                 dapE 
                 Succinyldiaminopimelate desuccinylase 
                 Wehrmann, A. et al. “Analysis of different DNA fragments of 
               
               
                   
                   
                   
                   Corynebacterium glutamicum  complementing dapE of  Escherichia coli ,” 
               
               
                   
                   
                   
                 Microbiology, 40: 3349-56 (1994) 
               
               
                 X82061 
                 16S rDNA 
                 16S ribosomal RNA 
                 Ruimy, R. et al. “Phylogeny of the genus  Corynebacterium  deduced from 
               
               
                   
                   
                   
                 analyses of small-subunit ribosomal DNA sequences,” Int. J. Syst. Bacteriol., 
               
               
                   
                   
                   
                 45(4): 740-746 (1995) 
               
               
                 X82928 
                 asd; lysC 
                 Aspartate-semialdehyde dehydrogenase; ? 
                 Serebrijski, I. et al. “Multicopy suppression by asd gene and osmotic stress- 
               
               
                   
                   
                   
                 dependent complementation by heterologous proA in proA mutants,” J. 
               
               
                   
                   
                   
                 Bacteriol., 177(24): 7255-7260 (1995) 
               
               
                 X82929 
                 proA 
                 Gamma-glutamyl phosphate reductase 
                 Serebrijski, I. et al. “Multicopy suppression by asd gene and osmotic stress- 
               
               
                   
                   
                   
                 dependent complementation by heterologous proA in proA mutants,” J. 
               
               
                   
                   
                   
                 Bacteriol., 177(24): 7255-7260 (1995) 
               
               
                 X84257 
                 16S rDNA 
                 16S ribosomal RNA 
                 Pascual, C. et al. “Phylogenetic analysis of the genus  Corynebacterium  based 
               
               
                   
                   
                   
                 on 16S rRNA gene sequences,” Int. J. Syst. Bacteriol., 45(4): 724-728 (1995) 
               
               
                 X85965 
                 aroP; dapE 
                 Aromatic amino acid permease; ? 
                 Wehrmann, A. et al. “Functional analysis of sequences adjacent to dapE of 
               
               
                   
                   
                   
                   Corynebacterium glutamicum  proline reveals the presence of aroP, which 
               
               
                   
                   
                   
                 encodes the aromatic amino acid transporter,” J. Bacteriol., 177(20): 5991-5993 
               
               
                   
                   
                   
                 (1995) 
               
               
                 X86157 
                 argB; argC; argD; 
                 Acetylglutamate kinase; N-acetyl-gamma- 
                 Sakanyan, V. et al. “Genes and enzymes of the acetyl cycle of arginine 
               
               
                   
                 argF; argJ 
                 glutamyl-phosphate reductase; 
                 biosynthesis in  Corynebacterium glutamicum : enzyme evolution in the early 
               
               
                   
                   
                 acetylornithine aminotransferase; ornithine 
                 steps of the arginine pathway,” Microbiology, 142: 99-108 (1996) 
               
               
                   
                   
                 carbamoyltransferase; glutamate N- 
               
               
                   
                   
                 acetyltransferase 
               
               
                 X89084 
                 pta; ackA 
                 Phosphate acetyltransferase; acetate kinase 
                 Reinscheid, D. J. et al. “Cloning, sequence analysis, expression and inactivation 
               
               
                   
                   
                   
                 of the  Corynebacterium glutamicum  pta-ack operon encoding 
               
               
                   
                   
                   
                 phosphotransacetylase and acetate kinase,” Microbiology, 145: 503-513 (1999) 
               
               
                 X89850 
                 attB 
                 Attachment site 
                 Le Marrec, C. et al. “Genetic characterization of site-specific integration 
               
               
                   
                   
                   
                 functions of phi AAU2 infecting “Arthrobacter aureus C70,” J. Bacteriol., 
               
               
                   
                   
                   
                 178(7): 1996-2004 (1996) 
               
               
                 X90356 
                   
                 Promoter fragment F1 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90357 
                   
                 Promoter fragment F2 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90358 
                   
                 Promoter fragment F10 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90359 
                   
                 Promoter fragment F13 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90360 
                   
                 Promoter fragment F22 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90361 
                   
                 Promoter fragment F34 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90362 
                   
                 Promoter fragment F37 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90363 
                   
                 Promoter fragment F45 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90364 
                   
                 Promoter fragment F64 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90365 
                   
                 Promoter fragment F75 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90366 
                   
                 Promoter fragment PF101 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90367 
                   
                 Promoter fragment PF104 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X90368 
                   
                 Promoter fragment PF109 
                 Patek, M. et al. “Promoters from  Corynebacterium glutamicum : cloning, 
               
               
                   
                   
                   
                 molecular analysis and search for a consensus motif,” Microbiology, 
               
               
                   
                   
                   
                 142: 1297-1309 (1996) 
               
               
                 X93513 
                 amt 
                 Ammonium transport system 
                 Siewe, R. M. et al. “Functional and genetic characterization of the (methyl) 
               
               
                   
                   
                   
                 ammonium uptake carrier of  Corynebacterium glutamicum ,” J. Biol. Chem., 
               
               
                   
                   
                   
                 271(10): 5398-5403 (1996) 
               
               
                 X93514 
                 betP 
                 Glycine betaine transport system 
                 Peter, H. et al. “Isolation, characterization, and expression of the 
               
               
                   
                   
                   
                   Corynebacterium glutamicum  betP gene, encoding the transport system for the 
               
               
                   
                   
                   
                 compatible solute glycine betaine,” J. Bacteriol., 178(17): 5229-5234 (1996) 
               
               
                 X95649 
                 orf4 
                   
                 Patek, M. et al. “Identification and transcriptional analysis of the dapB-ORF2- 
               
               
                   
                   
                   
                 dapA-ORF4 operon of  Corynebacterium glutamicum , encoding two enzymes 
               
               
                   
                   
                   
                 involved in L-lysine synthesis,” Biotechnol. Lett., 19: 1113-1117 (1997) 
               
               
                 X96471 
                 lysE; lysG 
                 Lysine exporter protein; Lysine export 
                 Vrljic, M. et al. “A new type of transporter with a new type of cellular 
               
               
                   
                   
                 regulator protein 
                 function: L-lysine export from  Corynebacterium glutamicum ,” Mol. 
               
               
                   
                   
                   
                 Microbiol., 22(5): 815-826 (1996) 
               
               
                 X96580 
                 panB; panC; xylB 
                 3-methyl-2-oxobutanoate 
                 Sahm, H. et al. “D-pantothenate synthesis in  Corynebacterium glutamicum  and 
               
               
                   
                   
                 hydroxymethyltransferase; pantoate-beta- 
                 use of panBC and genes encoding L-valine synthesis for D-pantothenate 
               
               
                   
                   
                 alanine ligase; xylulokinase 
                 overproduction,” Appl. Environ. Microbiol., 65(5): 1973-1979 (1999) 
               
               
                 X96962 
                   
                 Insertion sequence IS1207 and transposase 
               
               
                 X99289 
                   
                 Elongation factor P 
                 Ramos, A. et al. “Cloning, sequencing and expression of the gene encoding 
               
               
                   
                   
                   
                 elongation factor P in the amino-acid producer  Brevibacterium lactofermentum    
               
               
                   
                   
                   
                 ( Corynebacterium glutamicum  ATCC 13869),” Gene, 198: 217-222 (1997) 
               
               
                 Y00140 
                 thrB 
                 Homoserine kinase 
                 Mateos, L. M. et al. “Nucleotide sequence of the homoserine kinase (thrB) gene 
               
               
                   
                   
                   
                 of the  Brevibacterium lactofermentum ,” Nucleic Acids Res., 15(9): 3922 (1987) 
               
               
                 Y00151 
                 ddh 
                 Meso-diaminopimelate D-dehydrogenase 
                 Ishino, S. et al. “Nucleotide sequence of the meso-diaminopimelate D- 
               
               
                   
                   
                 (EC 1.4.1.16) 
                 dehydrogenase gene from  Corynebacterium glutamicum ,” Nucleic Acids Res., 
               
               
                   
                   
                   
                 15(9): 3917 (1987) 
               
               
                 Y00476 
                 thrA 
                 Homoserine dehydrogenase 
                 Mateos, L. M. et al. “Nucleotide sequence of the homoserine dehydrogenase 
               
               
                   
                   
                   
                 (thrA) gene of the  Brevibacterium lactofermentum ,” Nucleic Acids Res., 
               
               
                   
                   
                   
                 15(24): 10598 (1987) 
               
               
                 Y00546 
                 hom; thrB 
                 Homoserine dehydrogenase; homoserine 
                 Peoples, O. P. et al. “Nucleotide sequence and fine structural analysis of the 
               
               
                   
                   
                 kinase 
                   Corynebacterium glutamicum  hom-thrB operon,” Mol. Microbiol., 2(1): 63-72 
               
               
                   
                   
                   
                 (1988) 
               
               
                 Y08964 
                 murC; ftsQ/divD; ftsZ 
                 UPD-N-acetylmuramate-alanine ligase; 
                 Honrubia, M. P. et al. “Identification, characterization, and chromosomal 
               
               
                   
                   
                 division initiation protein or cell division 
                 organization of the ftsZ gene from  Brevibacterium lactofermentum ,” Mol. Gen. 
               
               
                   
                   
                 protein; cell division protein 
                 Genet., 259(1): 97-104 (1998) 
               
               
                 Y09163 
                 putP 
                 High affinity proline transport system 
                 Peter, H. et al. “Isolation of the putP gene of  Corynebacterium   
               
               
                   
                   
                   
                   glutamicumproline  and characterization of a low-affinity uptake system for 
               
               
                   
                   
                   
                 compatible solutes,” Arch. Microbiol., 168(2): 143-151 (1997) 
               
               
                 Y09548 
                 pyc 
                 Pyruvate carboxylase 
                 Peters-Wendisch, P. G. et al. “Pyruvate carboxylase from  Corynebacterium   
               
               
                   
                   
                   
                   glutamicum : characterization, expression and inactivation of the pyc gene,” 
               
               
                   
                   
                   
                 Microbiology, 144: 915-927 (1998) 
               
               
                 Y09578 
                 leuB 
                 3-isopropylmalate dehydrogenase 
                 Patek, M. et al. “Analysis of the leuB gene from  Corynebacterium   
               
               
                   
                   
                   
                   glutamicum ,” Appl. Microbiol. Biotechnol., 50(1): 42-47 (1998) 
               
               
                 Y12472 
                   
                 Attachment site bacteriophage Phi-16 
                 Moreau, S. et al. “Site-specific integration of corynephage Phi-16: The 
               
               
                   
                   
                   
                 construction of an integration vector,” Microbiol., 145: 539-548 (1999) 
               
               
                 Y12537 
                 proP 
                 Proline/ectoine uptake system protein 
                 Peter, H. et al. “ Corynebacterium glutamicum  is equipped with four secondary 
               
               
                   
                   
                   
                 carriers for compatible solutes: Identification, sequencing, and characterization 
               
               
                   
                   
                   
                 of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine 
               
               
                   
                   
                   
                 betaine carrier, EctP,” J. Bacteriol., 180(22): 6005-6012 (1998) 
               
               
                 Y13221 
                 glnA 
                 Glutamine synthetase I 
                 Jakoby, M. et al. “Isolation of  Corynebacterium glutamicum  glnA gene 
               
               
                   
                   
                   
                 encoding glutamine synthetase I,” FEMS Microbiol. Lett., 154(1): 81-88 (1997) 
               
               
                 Y16642 
                 lpd 
                 Dihydrolipoamide dehydrogenase 
               
               
                 Y18059 
                   
                 Attachment site Corynephage 304L 
                 Moreau, S. et al. “Analysis of the integration functions of &amp;phi; 304L: An 
               
               
                   
                   
                   
                 integrase module among corynephages,” Virology, 255(1): 150-159 (1999) 
               
               
                 Z21501 
                 argS; lysA 
                 Arginyl-tRNA synthetase; diaminopimelate 
                 Oguiza, J. A. et al. “A gene encoding arginyl-tRNA synthetase is located in the 
               
               
                   
                   
                 decarboxylase (partial) 
                 upstream region of the lysA gene in  Brevibacterium lactofermentum : 
               
               
                   
                   
                   
                 Regulation of argS-lysA cluster expression by arginine,” J. Bacteriol., 
               
               
                   
                   
                   
                 175(22): 7356-7362 (1993) 
               
               
                 Z21502 
                 dapA; dapB 
                 Dihydrodipicolinate synthase; 
                 Pisabarro, A. et al. “A cluster of three genes (dapA, orf2, and dapB) of 
               
               
                   
                   
                 dihydrodipicolinate reductase 
                   Brevibacterium lactofermentum  encodes dihydrodipicolinate reductase, and a 
               
               
                   
                   
                   
                 third polypeptide of unknown function,” J. Bacteriol., 175(9): 2743-2749 
               
               
                   
                   
                   
                 (1993) 
               
               
                 Z29563 
                 thrC 
                 Threonine synthase 
                 Malumbres, M. et al. “Analysis and expression of the thrC gene of the encoded 
               
               
                   
                   
                   
                 threonine synthase,” Appl. Environ. Microbiol., 60(7)2209-2219 (1994) 
               
               
                 Z46753 
                 16S rDNA 
                 Gene for 16S ribosomal RNA 
               
               
                 A09073 
                 ppg 
                 Phosphoenol pyruvate carboxylase 
                 Bachmann, B. et al. “DNA fragment coding for phosphoenolpyruvat 
               
               
                 Z49822 
                 sigA 
                 SigA sigma factor 
                 Oguiza, J. A. et al “Multiple sigma factor genes in  Brevibacterium   
               
               
                   
                   
                   
                   lactofermentum : Characterization of sigA and sigB,” J. Bacteriol., 178(2): 550-553 
               
               
                   
                   
                   
                 (1996) 
               
               
                 Z49823 
                 galE; dtxR 
                 Catalytic activity UDP-galactose 4- 
                 Oguiza, J. A. et al “The galE gene encoding the UDP-galactose 4-epimerase of 
               
               
                   
                   
                 epimerase; diphtheria toxin regulatory 
                   Brevibacterium lactofermentum  is coupled transcriptionally to the dmdR 
               
               
                   
                   
                 protein 
                 gene,” Gene, 177: 103-107 (1996) 
               
               
                 Z49824 
                 orfl; sigB 
                 ?; SigB sigma factor 
                 Oguiza, J. A. et al “Multiple sigma factor genes in  Brevibacterium   
               
               
                   
                   
                   
                   lactofermentum : Characterization of sigA and sigB,” J. Bacteriol., 178(2): 550-553 
               
               
                   
                   
                   
                 (1996) 
               
               
                 Z66534 
                   
                 Transposase 
                 Correia, A. et al. “Cloning and characterization of an IS-like element present in 
               
               
                   
                   
                   
                 the genome of  Brevibacterium lactofermentum  ATCC 13869,” Gene, 
               
               
                   
                   
                   
                 170(1): 91-94 (1996) 
               
               
                   
               
               
                     1 A sequence for this gene was published in the indicated reference. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.    
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                   
               
               
                   Corynebacterium  and  Brevibacterium  Strains Which May be Used in the Practice of the Invention 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                 Genus 
                 species 
                 ATCC 
                 FERM 
                 NRRL 
                 CECT 
                 NCIMB 
                 CBS 
                 NCTC 
                 DSMZ 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 21054 
                   
                   
                   
                   
                   
                   
                   
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 19350 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 19351 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 19352 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 19353 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 19354 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 19355 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 19356 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 21055 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 21077 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 21553 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 21580 
               
               
                 
                   Brevibacterium 
                 
                 
                   ammoniagenes 
                 
                 39101 
               
               
                 
                   Brevibacterium 
                 
                 
                   butanicum 
                 
                 21196 
               
               
                 
                   Brevibacterium 
                 
                 
                   divaricatum 
                 
                 21792 
                 P928 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21474 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21129 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21518 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                   
                   
                 B11474 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                   
                   
                 B11472 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21127 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21128 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21427 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21475 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21517 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21528 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21529 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                   
                   
                 B11477 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                   
                   
                 B11478 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                 21127 
               
               
                 
                   Brevibacterium 
                 
                 
                   flavum 
                 
                   
                   
                 B11474 
               
               
                 
                   Brevibacterium 
                 
                 
                   healii 
                 
                 15527 
               
               
                 
                   Brevibacterium 
                 
                 
                   ketoglutamicum 
                 
                 21004 
               
               
                 
                   Brevibacterium 
                 
                 
                   ketoglutamicum 
                 
                 21089 
               
               
                 
                   Brevibacterium 
                 
                 
                   ketosoreductum 
                 
                 21914 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                   
                   
                   
                 70 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                   
                   
                   
                 74 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                   
                   
                   
                 77 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                 21798 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                 21799 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                 21800 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                 21801 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                   
                   
                 B11470 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                   
                   
                 B11471 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                 21086 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                 21420 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                 21086 
               
               
                 
                   Brevibacterium 
                 
                 
                   lactofermentum 
                 
                 31269 
               
               
                 
                   Brevibacterium 
                 
                 
                   linens 
                 
                 9174 
               
               
                 
                   Brevibacterium 
                 
                 
                   linens 
                 
                 19391 
               
               
                 
                   Brevibacterium 
                 
                 
                   linens 
                 
                 8377 
               
               
                 
                   Brevibacterium 
                 
                 
                   paraffinolyticum 
                 
                   
                   
                   
                   
                 11160 
               
               
                 
                   Brevibacterium 
                 
                 spec. 
                   
                   
                   
                   
                   
                 717.73 
               
               
                 
                   Brevibacterium 
                 
                 spec. 
                   
                   
                   
                   
                   
                 717.73 
               
               
                 
                   Brevibacterium 
                 
                 spec. 
                 14604 
               
               
                 
                   Brevibacterium 
                 
                 spec. 
                 21860 
               
               
                 
                   Brevibacterium 
                 
                 spec. 
                 21864 
               
               
                 
                   Brevibacterium 
                 
                 spec. 
                 21865 
               
               
                 
                   Brevibacterium 
                 
                 spec. 
                 21866 
               
               
                 
                   Brevibacterium 
                 
                 spec. 
                 19240 
               
               
                 
                   Corynebacterium 
                 
                 
                   acetoacidophilum 
                 
                 21476 
               
               
                 
                   Corynebacterium 
                 
                 
                   acetoacidophilum 
                 
                 13870 
               
               
                 
                   Corynebacterium 
                 
                 
                   acetoglutamicum 
                 
                   
                   
                 B11473 
               
               
                 
                   Corynebacterium 
                 
                 
                   acetoglutamicum 
                 
                   
                   
                 B11475 
               
               
                 
                   Corynebacterium 
                 
                 
                   acetoglutamicum 
                 
                 15806 
               
               
                 
                   Corynebacterium 
                 
                 
                   acetoglutamicum 
                 
                 21491 
               
               
                 
                   Corynebacterium 
                 
                 
                   acetoglutamicum 
                 
                 31270 
               
               
                 
                   Corynebacterium 
                 
                 
                   acetophilum 
                 
                   
                   
                 B3671 
               
               
                 
                   Corynebacterium 
                 
                 
                   ammoniagenes 
                 
                 6872 
                   
                   
                   
                   
                   
                 2399 
               
               
                 
                   Corynebacterium 
                 
                 
                   ammoniagenes 
                 
                 15511 
               
               
                 
                   Corynebacterium 
                 
                 
                   fujiokense 
                 
                 21496 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 14067 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 39137 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21254 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21255 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 31830 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 13032 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 14305 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 15455 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 13058 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 13059 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 13060 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21492 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21513 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21526 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21543 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 13287 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21851 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21253 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21514 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21516 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21299 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21300 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 39684 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21488 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21649 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21650 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19223 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 13869 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21157 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21158 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21159 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21355 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 31808 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21674 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21562 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21563 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21564 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21565 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21566 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21567 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21568 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21569 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21570 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21571 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21572 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21573 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21579 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19049 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19050 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19051 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19052 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19053 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19054 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19055 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19056 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19057 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19058 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19059 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19060 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 19185 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 13286 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21515 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21527 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21544 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21492 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                   
                   
                 B8183 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                   
                   
                 B8182 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                   
                   
                 B12416 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                   
                   
                 B12417 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                   
                   
                 B12418 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                   
                   
                 B11476 
               
               
                 
                   Corynebacterium 
                 
                 
                   glutamicum 
                 
                 21608 
               
               
                 
                   Corynebacterium 
                 
                 
                   lilium 
                 
                   
                 P973 
               
               
                 
                   Corynebacterium 
                 
                 
                   nitrilophilus 
                 
                 21419 
                   
                   
                   
                 11594 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                   
                 P4445 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                   
                 P4446 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 31088 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 31089 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 31090 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 31090 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 31090 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 15954 
                   
                   
                   
                   
                   
                   
                 20145 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 21857 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 21862 
               
               
                 
                   Corynebacterium 
                 
                 spec. 
                 21863 
               
               
                   
               
               
                   ATCC: American Type Culture Collection, Rockville, MD, USA    
               
               
                   FERM: Fermentation Research Institute, Chiba, Japan    
               
               
                   NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, IL, USA    
               
               
                   CECT: Coleccion Espanola de Cultivos Tipo, Valencia, Spain    
               
               
                   NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, UK    
               
               
                   CBS: Centraalbureau voor Schimmelcultures, Baarn, NL    
               
               
                   NCTC: National Collection of Type Cultures, London, UK    
               
               
                   DSMZ: Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany    
               
               
                   For reference see Sugawara, H. et al. (1993) World directory of collections of cultures of microorganisms: Bacteria, fungi and yeasts (4 th  edn), World federation for culture collections world data center on microorganisms, Saimata, Japen.    
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                   
               
               
                 ALIGNMENT RESULTS 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                   
                   
                 % 
                   
               
               
                   
                 length 
                   
                   
                   
                   
                   
                 homology 
                 Date of 
               
               
                 ID # 
                 (NT) 
                 Genbank Hit 
                 Length 
                 Accession 
                 Name of Genbank Hit 
                 Source of Genbank Hit 
                 (GAP) 
                 Deposit 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 rxa00051 
                 1527 
                 GB_HTG3: AC009685 
                 210031 
                 AC009685 
                   Homo sapiens  chromosome 15 clone 91_E_13 map 15, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 34,247 
                 29-Sep-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 27 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC009685 
                 210031 
                 AC009685 
                   Homo sapiens  chromosome 15 clone 91_E_13 map 15, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 34,247 
                 29-Sep-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 27 unordered pieces. 
               
               
                   
                   
                 GB_HTG7: AC009511 
                 271896 
                 AC009511 
                   Homo sapiens  clone RP11-860B13, *** SEQUENCING IN PROGRESS ***, 59 
                 
                   Homo sapiens 
                 
                 35,033 
                 09-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa00091 
                 876 
                 GB_BA1: D50453 
                 146191 
                 D50453 
                   Bacillus subtilis  DNA for 25-36 degree region containing the amyE-srfA region, 
                 
                   Bacillus subtilis 
                 
                 54,452 
                 10-Feb-99 
               
               
                   
                   
                   
                   
                   
                 complete cds. 
               
               
                   
                   
                 GB_BA1: SCI51 
                 40745 
                 AL109848 
                   Streptomyces coelicolor  cosmid I51. 
                 
                   Streptomyces coelicolor 
                 
                 36,806 
                 16-Aug-99 
               
               
                   
                   
                   
                   
                   
                   
                 A3(2) 
               
               
                   
                   
                 GB_BA1: ECOUW93 
                 338534 
                 U14003 
                   Escherichia coli  K-12 chromosomal region from 92.8 to 00.1 minutes. 
                 
                   Escherichia coli 
                 
                 38,642 
                 17-Apr-96 
               
               
                 rxa00092 
                 789 
                 GB_BA1: SCH35 
                 45396 
                 AL078610 
                   Streptomyces coelicolor  cosmid H35. 
                 
                   Streptomyces coelicolor 
                 
                 49,934 
                 4-Jun-99 
               
               
                   
                   
                 GB_HTG3: AC011498_0 
                 312343 
                 AC011498 
                   Homo sapiens  chromosome 19 clone CIT978SKB_50L17, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 37,117 
                 13-Dec-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 190 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC011498_0 
                 312343 
                 AC011498 
                   Homo sapiens  chromosome 19 clone CIT978SKB_50L17, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 37,117 
                 13-Dec-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 190 unordered pieces. 
               
               
                 rxa00104 
                 879 
                 GB_BA1: MTCY270 
                 37586 
                 Z95388 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 96/162. 
                 
                   Mycobacterium 
                 
                 36,732 
                 10-Feb-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_PL2: T24M8 
                 68251 
                 AF077409 
                   Arabidopsis thaliana  BAC T24M8. 
                 
                   Arabidopsis thaliana 
                 
                 37,150 
                 3-Aug-98 
               
               
                   
                   
                 GB_BA1: MTCY270 
                 37586 
                 Z95388 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 96/162. 
                 
                   Mycobacterium 
                 
                 42,874 
                 10-Feb-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                 rxa00113 
                 5745 
                 GB_BA1: MAFASGEN 
                 10520 
                 X87822 
                   B. ammoniagenes  FAS gene. 
                 
                   Corynebacterium 
                 
                 68,381 
                 03-OCT-1996 
               
               
                   
                   
                   
                   
                   
                   
                 
                   ammoniagenes 
                 
               
               
                   
                   
                 GB_BA1: BAFASAA 
                 10549 
                 X64795 
                   B. ammoniagenes  FAS gene. 
                 
                   Corynebacterium 
                 
                 57,259 
                 14-OCT-1997 
               
               
                   
                   
                   
                   
                   
                   
                 
                   ammoniagenes 
                 
               
               
                   
                   
                 GB_BA1: MTCY159 
                 33818 
                 Z83863 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 111/162. 
                 
                   Mycobacterium 
                 
                 39,870 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                 rxa00164 
                 1812 
                 GB_HTG2: HSJ1153D9 
                 118360 
                 AL109806 
                   Homo sapiens  chromosome 20 clone RP5-1153D9, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 35,714 
                 03-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, in unordered pieces. 
               
               
                   
                   
                 GB_HTG2: HSJ1153D9 
                 118360 
                 AL109806 
                   Homo sapiens  chromosome 20 clone RP5-1153D9, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 35,714 
                 03-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, in unordered pieces. 
               
               
                   
                   
                 GB_HTG2: HSJ1153D9 
                 118360 
                 AL109806 
                   Homo sapiens  chromosome 20 clone RP5-1153D9, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 35,334 
                 03-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, in unordered pieces. 
               
               
                 rxa00181 
                 1695 
                 GB_BA1: CGPUTP 
                 3791 
                 Y09163 
                   C. glutamicum  putP gene. 
                 
                   Corynebacterium 
                 
                 100,000 
                 8-Sep-97 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA2: U32814 
                 10393 
                 U32814 
                   Haemophilus influenzae  Rd section 129 of 163 of the complete genome. 
                 
                   Haemophilus influenzae 
                 
                 36,347 
                 29-MAY-1998 
               
               
                   
                   
                   
                   
                   
                   
                 Rd 
               
               
                   
                   
                 GB_BA1: CGPUTP 
                 3791 
                 Y09163 
                   C. glutamicum  putP gene. 
                 
                   Corynebacterium 
                 
                 37,454 
                 8-Sep-97 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                 rxa00186 
                 870 
                 GB_PR3: AC004843 
                 136655 
                 AC004843 
                   Homo sapiens  PAC clone DJ0612F12 from 7p12-p14, complete sequence. 
                 
                   Homo sapiens 
                 
                 37,315 
                 5-Nov-98 
               
               
                   
                   
                 GB_HTG2: HS745I14 
                 133309 
                 AL033532 
                   Homo sapiens  chromosome 1 clone RP4-745I14 map q23.1-24.3, *** SEQUENCING 
                 
                   Homo sapiens 
                 
                 38,129 
                 03-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 IN PROGRESS ***, in unordered pieces. 
               
               
                   
                   
                 GB_HTG2: HS745I14 
                 133309 
                 AL033532 
                   Homo sapiens  chromosome 1 clone RP4-745I14 map q23.1-24.3, *** SEQUENCING 
                 
                   Homo sapiens 
                 
                 38,129 
                 03-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 IN PROGRESS ***, in unordered pieces. 
               
               
                 rxa00187 
                 474 
                 GB_GSS10: AQ184082 
                 506 
                 AQ184082 
                 HS_3216_A1_G08_T7 CIT Approved Human Genomic Sperm Library D  Homo   
                 
                   Homo sapiens 
                 
                 37,297 
                 1-Nov-98 
               
               
                   
                   
                   
                   
                   
                   sapiens  genomic clone Plate = 3216 Col = 15 Row = M, genomic survey sequence. 
               
               
                   
                   
                 GB_GSS1: CNS008ZZ 
                 1101 
                 AL052951 
                   Drosophila melanogaster  genome survey sequence T7 end of BAC # BACR18L01 of 
                 
                   Drosophila melanogaster 
                 
                 34,120 
                 3-Jun-99 
               
               
                   
                   
                   
                   
                   
                 RPCI-98 library from  Drosophila melanogaster  (fruit fly), genomic survey sequence. 
               
               
                   
                   
                 GB_GSS10: AQ184082 
                 506 
                 AQ184082 
                 HS_3216_A1_G08_T7 CIT Approved Human Genomic Sperm Library D  Homo   
                 
                   Homo sapiens 
                 
                 39,655 
                 1-Nov-98 
               
               
                   
                   
                   
                   
                   
                   sapiens  genomic clone Plate = 3216 Col = 15 Row = M, genomic survey sequence. 
               
               
                 rxa00201 
                 292 
                 GB_PR3: HSJ824F16 
                 139330 
                 AL050325 
                 Human DNA sequence from clone 824F16 on chromosome 20, complete sequence. 
                 
                   Homo sapiens 
                 
                 34,520 
                 23-Nov-99 
               
               
                   
                   
                 GB_BA1: RCSECA 
                 2724 
                 X89411 
                   R. capsulatus  DNA for secA gene. 
                 
                   Rhodobacter capsulatus 
                 
                 38,163 
                 6-Jan-96 
               
               
                   
                   
                 GB_EST34: AV122904 
                 242 
                 AV122904 
                 AV122904  Mus musculus  C57BL/6J 10-day embryo  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 38,889 
                 1-Jul-99 
               
               
                   
                   
                   
                   
                   
                 2610529H07, mRNA sequence. 
               
               
                 rxa00228 
                 714 
                 GB_EST15: AA486042 
                 515 
                 AA486042 
                 ab40c08.r1 Stratagene HeLa cell s3 937216  Homo sapiens  cDNA clone 
                 
                   Homo sapiens 
                 
                 37,500 
                 06-MAR-1998 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 843278 5′, mRNA sequence. 
               
               
                   
                   
                 GB_EST15: AA486042 
                 515 
                 AA486042 
                 ab40c08.r1 Stratagene HeLa cell s3 937216  Homo sapiens  cDNA clone 
                 
                   Homo sapiens 
                 
                 38,816 
                 06-MAR-1998 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 843278 5′, mRNA sequence. 
               
               
                 rxa00243 
                 1140 
                 GB_PR2: CNS01DS5 
                 101584 
                 AL121655 
                 BAC sequence from the SPG4 candidate region at 2p21-2p22, complete sequence. 
                 
                   Homo sapiens 
                 
                 37,001 
                 29-Sep-99 
               
               
                   
                   
                 GB_HTG3: AC011408 
                 79332 
                 AC011408 
                   Homo sapiens  clone CIT978SKB_65D22, 
                 
                   Homo sapiens 
                 
                 38,040 
                 06-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 *** SEQUENCING IN PROGRESS ***, 10 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC011408 
                 79332 
                 AC011408 
                   Homo sapiens  clone CIT978SKB_65D22, 
                 
                   Homo sapiens 
                 
                 38,040 
                 06-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 *** SEQUENCING IN PROGRESS ***, 10 unordered pieces. 
               
               
                 rxa00259 
                 2325 
                 GB_HTG1: CEY62E10 
                 254217 
                 AL031580 
                   Caenorhabditis elegans  chromosome IV clone Y62E10, *** SEQUENCING IN 
                 
                   Caenorhabditis elegans 
                 
                 36,776 
                 6-Sep-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, in unordered pieces. 
               
               
                   
                   
                 GB_HTG1: CEY62E10 
                 254217 
                 AL031580 
                   Caenorhabditis elegans  chromosome IV clone Y62E10, *** SEQUENCING IN 
                 
                   Caenorhabditis elegans 
                 
                 36,776 
                 6-Sep-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, in unordered pieces. 
               
               
                   
                   
                 GB_PL2: YSCCHROMI 
                 41988 
                 L22015 
                   Saccharomyces cerevisiae  chromosome I centromere and right arm sequence. 
                 
                   Saccharomyces 
                 
                 39,260 
                 05-MAR-1998 
               
               
                   
                   
                   
                   
                   
                   
                 
                   cerevisiae 
                 
               
               
                 rxa00269 
                 912 
                 GB_HTG4: AC009974 
                 219565 
                 AC009974 
                   Homo sapiens  chromosome unknown clone NH0459I19, WORKING DRAFT 
                 
                   Homo sapiens 
                 
                 37,358 
                 29-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 SEQUENCE, in unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC009974 
                 219565 
                 AC009974 
                   Homo sapiens  chromosome unknown clone NH0459I19, WORKING DRAFT 
                 
                   Homo sapiens 
                 
                 37,358 
                 29-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 SEQUENCE, in unordered pieces. 
               
               
                   
                   
                 GB_BA1: AB017508 
                 32050 
                 AB017508 
                   Bacillus halodurans  C-125 genomic DNA, 32 kb fragment, complete cds. 
                 
                   Bacillus halodurans 
                 
                 44,622 
                 14-Apr-99 
               
               
                 rxa00281 
                 766 
                 GB_BA1: SCE8 
                 24700 
                 AL035654 
                   Streptomyces coelicolor  cosmid E8. 
                 
                   Streptomyces coelicolor 
                 
                 36,328 
                 11-MAR-1999 
               
               
                   
                   
                 GB_BA1: SCU51332 
                 3216 
                 U51332 
                   Streptomyces coelicolor  histidine kinase homolog (absA1) and response regulator 
                 
                   Streptomyces coelicolor 
                 
                 39,089 
                 14-Sep-96 
               
               
                   
                   
                   
                   
                   
                 homolog (absA2) genes, complete cds. 
               
               
                   
                   
                 GB_HTG4: AC011122 
                 187123 
                 AC011122 
                   Homo sapiens  chromosome 8 clone 23_D_19 map 8, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 38,658 
                 14-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 27 ordered pieces. 
               
               
                 rxa00298 
                 1968 
                 GB_BA1: CGECTP 
                 2719 
                 AJ001436 
                   Corynebacterium glutamicum  ectP gene. 
                 
                   Corynebacterium 
                 
                 100,000 
                 20-Nov-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: CGECTP 
                 2719 
                 AJ001436 
                   Corynebacterium glutamicum  ectP gene. 
                 
                   Corynebacterium 
                 
                 100,000 
                 20-Nov-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_EST24: AI234006 
                 432 
                 AI234006 
                 EST230694 Normalized rat lung, Bento Soares  Rattus  sp. cDNA clone RLUCU01 3′ 
                   Rattus  sp. 
                 46,552 
                 31-Jan-99 
               
               
                   
                   
                   
                   
                   
                 end, mRNA sequence. 
               
               
                 rxa00346 
                 813 
                 GB_BA1: SC2E9 
                 20850 
                 AL021530 
                   Streptomyces coelicolor  cosmid 2E9. 
                 
                   Streptomyces coelicolor 
                 
                 43,267 
                 28-Jan-98 
               
               
                   
                   
                 GB_BA1: SC9B1 
                 24800 
                 AL049727 
                   Streptomyces coelicolor  cosmid 9B1. 
                 
                   Streptomyces coelicolor 
                 
                 44,613 
                 27-Apr-99 
               
               
                   
                   
                 GB_BA1: ECU70214 
                 123171 
                 U70214 
                   Escherichia coli  chromosome minutes 4-6. 
                 
                   Escherichia coli 
                 
                 39,490 
                 21-Sep-96 
               
               
                 rxa00368 
                 1698 
                 GB_BA2: AF065159 
                 35209 
                 AF065159 
                   Bradyrhizobium japonicum  putative arylsulfatase (arsA), putative soluble lytic 
                 
                   Bradyrhizobium 
                 
                 40,409 
                 27-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 transglycosylase precursor (sltA), dihydrodipicolinate synthase (dapA), MscL (mscL), 
                 
                   japonicum 
                 
               
               
                   
                   
                   
                   
                   
                 SmpB (smpB), BcpB (bcpB), RnpO (rnpO), RelA/SpoT homolog (relA), PdxJ (pdxJ), 
               
               
                   
                   
                   
                   
                   
                 and acyl carrier protein synthase AcpS (acpS) genes, complete cds; prokaryotic type 
               
               
                   
                   
                   
                   
                   
                 I signal peptidase SipF (sipF) gene, sipF-sipS allele, complete cds; RNase III (rnc) 
               
               
                   
                   
                   
                   
                   
                 gene, complete cds; GTP-binding protein Era (era) gene, partial cds; and unknown 
               
               
                   
                   
                   
                   
                   
                 genes. 
               
               
                   
                   
                 GB_BA1: AEOCHIT1 
                 6861 
                 D63139 
                   Aeromonas  sp. gene for chitinase, complete and partial cds. 
                   Aeromonas  sp. 10S-24 
                 38,577 
                 13-Feb-99 
               
               
                   
                   
                 GB_EST4: D62996 
                 314 
                 D62996 
                 HUM347G01B Clontech human aorta polyA+ mRNA (#6572)  Homo sapiens  cDNA 
                 
                   Homo sapiens 
                 
                 41,613 
                 29-Aug-95 
               
               
                   
                   
                   
                   
                   
                 clone GEN-347G01 5′, mRNA sequence. 
               
               
                 rxa00369 
                 817 
                 GB_BA1: YP102KB 
                 119443 
                 AL031866 
                   Yersinia pestis  102 kbases unstable region: from 1 to 119443. 
                 
                   Yersinia pestis 
                 
                 35,396 
                 4-Jan-99 
               
               
                   
                   
                 GB_GSS8: AQ012142 
                 501 
                 AQ012142 
                 8750H1A037010398 Cosmid library of chromosome II  Rhodobacter sphaeroides   
                 
                   Rhodobacter sphaeroides 
                 
                 54,800 
                 4-Jun-98 
               
               
                   
                   
                   
                   
                   
                 genomic clone 8750H1A037010398, genomic survey sequence. 
               
               
                   
                   
                 GB_HTG2: AC005081 
                 180096 
                 AC005081 
                   Homo sapiens  clone RG270D13, *** SEQUENCING IN PROGRESS ***, 18 
                 
                   Homo sapiens 
                 
                 45,786 
                 12-Jun-98 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa00410 
                 789 
                 GB_BA1: ATPLOCC 
                 8870 
                 Z30328 
                   A. tumefaciens  Ti plasmid pTiAch5 genes for OccR, OccQ, OccM, OccP, OccT, 
                 
                   Agrobacterium 
                 
                 46,490 
                 10-OCT-1994 
               
               
                   
                   
                   
                   
                   
                 OoxB, OoxA and ornithine cyclodeaminase. 
                 
                   tumefaciens 
                 
               
               
                   
                   
                 GB_BA2: U67591 
                 9829 
                 U67591 
                   Methanococcus jannaschii  section 133 of 150 of the complete genome. 
                 
                   Methanococcus 
                 
                 45,677 
                 28-Jan-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   jannaschii 
                 
               
               
                   
                   
                 GB_BA1: TIPOCCQMPJ 
                 4350 
                 M80607 
                 Plasmid pTiA6 (from  Agribacterium tumefaciens ) periplasmic-type octopine 
                 Plasmid pTiA6 
                 46,490 
                 24-Apr-96 
               
               
                   
                   
                   
                   
                   
                 permease (occR, occQ, occM, occP, and occJ) and lysR-type regulatory protein 
               
               
                   
                   
                   
                   
                   
                 (occR) genes, complete cds. 
               
               
                 rxa00419 
                 882 
                 GB_BA2: MSU46844 
                 16951 
                 U46844 
                   Mycobacterium smegmatis  catalase-peroxidase (katG), putative arabinosyl 
                 
                   Mycobacterium 
                 
                 57,029 
                 12-MAY-1997 
               
               
                   
                   
                   
                   
                   
                 transferase (embC, embA, embB), genes complete cds and putative propionyl-coA 
                 
                   smegmatis 
                 
               
               
                   
                   
                   
                   
                   
                 carboxylase beta chain (pccB) genes, partial cds. 
               
               
                   
                   
                 GB_EST28: AI513245 
                 471 
                 AI513245 
                 GH13311.3prime GH  Drosophila melanogaster  head pOT2  Drosophila melanogaster   
                 
                   Drosophila melanogaster 
                 
                 37,696 
                 16-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 cDNA clone GH13311 3prime, mRNA sequence. 
               
               
                   
                   
                 GB_HTG4: AC010066 
                 187240 
                 AC010066 
                   Drosophila melanogaster  chromosome 3L/72A4 clone RPCI98-25O1, *** 
                 
                   Drosophila melanogaster 
                 
                 39,607 
                 16-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 SEQUENCING IN PROGRESS ***, 70 unordered pieces. 
               
               
                 rxa00432 
                 1608 
                 GB_BA1: BSUB0015 
                 218410 
                 Z99118 
                   Bacillus subtilis  complete genome (section 15 of 21): from 2795131 to 3013540. 
                 
                   Bacillus subtilis 
                 
                 49,810 
                 26-Nov-97 
               
               
                   
                   
                 GB_PL1: CAC35A5 
                 42565 
                 AL033396 
                   C. albicans  cosmid Ca35A5. 
                 
                   Candida albicans 
                 
                 35,041 
                 5-Nov-98 
               
               
                   
                   
                 GB_EST13: AA336266 
                 378 
                 AA336266 
                 EST40981 Endometrial tumor  Homo sapiens  cDNA 5′ end, mRNA sequence. 
                 
                   Homo sapiens 
                 
                 39,733 
                 21-Apr-97 
               
               
                 rxa00449 
                 1704 
                 GB_HTG2: AC008199 
                 124050 
                 AC008199 
                   Drosophila melanogaster  chromosome 3 clone BACR01K08 (D756) RPCI-98 01.K.8 
                 
                   Drosophila melanogaster 
                 
                 38,392 
                 2-Aug-99 
               
               
                   
                   
                   
                   
                   
                 map 94D-94D strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 83 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_HTG2: AC008199 
                 124050 
                 AC008199 
                   Drosophila melanogaster  chromosome 3 clone BACR01K08 (D756) RPCI-98 01.K.8 
                 
                   Drosophila melanogaster 
                 
                 38,392 
                 2-Aug-99 
               
               
                   
                   
                   
                   
                   
                 map 94D-94D strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 83 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_RO: RATLNKP2 
                 177 
                 M22337 
                 Rat link protein gene, exon 2. 
                   Rattus  sp. 
                 40,678 
                 27-Apr-93 
               
               
                 rxa00456 
                 1500 
                 GB_GSS1: FR0030597 
                 476 
                 AL026966 
                   Fugu rubripes  GSS sequence, clone 091C22aF9, genomic survey sequence. 
                 
                   Fugu rubripes 
                 
                 47,407 
                 25-Jun-98 
               
               
                   
                   
                 GB_GSS5: AQ786587 
                 556 
                 AQ786587 
                 HS_3086_B1_H05_MR CIT Approved Human Genomic Sperm Library D  Homo   
                 
                   Homo sapiens 
                 
                 38,406 
                 3-Aug-99 
               
               
                   
                   
                   
                   
                   
                   sapiens  genomic clone Plate = 3086 Col = 9 Row = P, genomic survey sequence. 
               
               
                   
                   
                 GB_GSS14: AQ526586 
                 434 
                 AQ526586 
                 HS_5198_B1_B03_SP6E RPCI-11 Human Male BAC Library Homo sapiens 
                 
                   Homo sapiens 
                 
                 36,951 
                 11-MAY-1999 
               
               
                   
                   
                   
                   
                   
                 genomic clone Plate = 774 Col = 5 Row = D, genomic survey sequence. 
               
               
                 rxa00477 
                 1767 
                 GB_EST17: AA610489 
                 407 
                 AA610489 
                 np93e05.s1 NCI_CGAP_Thy1  Homo sapiens  cDNA clone IMAGE: 1133888 similar 
                 
                   Homo sapiens 
                 
                 41,791 
                 09-DEC-1997 
               
               
                   
                   
                   
                   
                   
                 to gb: M11353 HISTONE H3.3 (HUMAN);, mRNA sequence. 
               
               
                   
                   
                 GB_PR1: HSH33G4 
                 1015 
                 X05857 
                 Human H3.3 gene exon 4. 
                 
                   Homo sapiens 
                 
                 38,182 
                 24-Jan-96 
               
               
                   
                   
                 GB_EST30: AI637667 
                 579 
                 AI637667 
                 tt10g11.x1 NCI_CGAP_GC6  Homo sapiens  cDNA clone IMAGE: 2240420 3′, 
                 
                   Homo sapiens 
                 
                 35,417 
                 27-Apr-99 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                 rxa00478 
                 954 
                 GB_HTG3: AC008708 
                 83932 
                 AC008708 
                   Homo sapiens  chromosome 5 clone CIT978SKB_78F1, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 38,769 
                 3-Aug-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 12 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC008708 
                 83932 
                 AC008708 
                   Homo sapiens  chromosome 5 clone CIT978SKB_78F1, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 38,769 
                 3-Aug-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 12 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC008708 
                 83932 
                 AC008708 
                   Homo sapiens  chromosome 5 clone CIT978SKB_78F1, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 36,797 
                 3-Aug-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 12 unordered pieces. 
               
               
                 rxa00480 
                 1239 
                 GB_HTG1: HSJ575L21 
                 94715 
                 AL096841 
                   Homo sapiens  chromosome 1 clone RP4-575L21, *** SEQUENCING IN PROGRESS 
                 
                   Homo sapiens 
                 
                 38,138 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 ***, In unordered pieces. 
               
               
                   
                   
                 GB_HTG1: HSJ575L21 
                 94715 
                 AL096841 
                   Homo sapiens  chromosome 1 clone RP4-575L21, *** SEQUENCING IN PROGRESS 
                 
                   Homo sapiens 
                 
                 38,138 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 ***, In unordered pieces. 
               
               
                   
                   
                 GB_RO: AC005960 
                 158414 
                 AC005960 
                   Mus musculus  chromosome 17 BAC cltb20h22 from the MHC region, complete 
                 
                   Mus musculus 
                 
                 38,712 
                 01-DEC-1998 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                 rxa00524 
                 433 
                 GB_BA1: SCI51 
                 40745 
                 AL109848 
                   Streptomyces coelicolor  cosmid I51. 
                 
                   Streptomyces coelicolor 
                 
                 40,284 
                 16-Aug-99 
               
               
                   
                   
                   
                   
                   
                   
                 A3(2) 
               
               
                   
                   
                 GB_BA2: AF082879 
                 3434 
                 AF082879 
                   Yersinia enterocolitica  ABC transporter enterochelin/enterobactin gene cluster, 
                 
                   Yersinia enterocolitica 
                 
                 55,634 
                 20-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 complete sequence. 
               
               
                   
                   
                 GB_BA1: BSP132617 
                 5192 
                 AJ132617 
                   Burkholderia  sp. P-transporter operon and flanking genes. 
                   Burkholderia  sp. 
                 40,793 
                 13-Jul-99 
               
               
                 rxa00526 
                 813 
                 GB_BA1: BSUB0008 
                 208230 
                 Z99111 
                   Bacillus subtilis  complete genome (section 8 of 21): from 1394791 to 1603020. 
                 
                   Bacillus subtilis 
                 
                 54,534 
                 26-Nov-97 
               
               
                   
                   
                 GB_BA2: AF012285 
                 46864 
                 AF012285 
                   Bacillus subtilis  mobA-nprE gene region. 
                 
                   Bacillus subtilis 
                 
                 54,534 
                 1-Jul-98 
               
               
                   
                   
                 GB_BA1: D90725 
                 13796 
                 D90725 
                   Escherichia coli  genomic DNA. (19.7-20.0 min). 
                 
                   Escherichia coli 
                 
                 51,481 
                 7-Feb-99 
               
               
                 rxa00559 
                 1140 
                 GB_BA2: CAU77910 
                 3385 
                 U77910 
                   Corynebacterium ammoniagenes  sequence upstream of the 5-phosphoribosyl-1- 
                 
                   Corynebacterium 
                 
                 39,007 
                 1-Jan-98 
               
               
                   
                   
                   
                   
                   
                 pyrophosphate amidotransferase (purF) gene. 
                 
                   ammoniagenes 
                 
               
               
                   
                   
                 GB_EST4: H34952 
                 382 
                 H34952 
                 EST108261 Rat PC-12 cells, untreated  Rattus  sp. cDNA clone RPCCK07 similar to 
                   Rattus  sp. 
                 39,267 
                 2-Apr-98 
               
               
                   
                   
                   
                   
                   
                 NADH-ubiquinone oxidoreductase complex I 23 kDa precursor (iron-sulfur protein), 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                   
                   
                 GB_BA2: AE000963 
                 22014 
                 AE000963 
                   Archaeoglobus fulgidus  section 144 of 172 of the complete genome. 
                 
                   Archaeoglobus fulgidus 
                 
                 38,338 
                 15-DEC-1997 
               
               
                 rxa00570 
                 852 
                 GB_GSS12: AQ422451 
                 563 
                 AQ422451 
                 RPCI-11-185C3.TV RPCI-11  Homo sapiens  genomic clone RPCI-11-185C3, 
                 
                   Homo sapiens 
                 
                 38,767 
                 23-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 genomic survey sequence. 
               
               
                   
                   
                 GB_EST28: AI504741 
                 568 
                 AI504741 
                 vl16c01.x1 Stratagene mouse Tcell 937311  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 37,900 
                 11-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 972384 3′ similar to gb: Z14044  M. musculus  mRNA for valosin-containing 
               
               
                   
                   
                   
                   
                   
                 protein (MOUSE);, mRNA sequence. 
               
               
                   
                   
                 GB_EST18: AA712043 
                 68 
                 AA712043 
                 vu29f10.r1 Barstead mouse myotubes MPLRB5  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 42,647 
                 24-DEC-1997 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 1182091 5′ similar to gb: L05093 60S RIBOSOMAL PROTEIN L18A 
               
               
                   
                   
                   
                   
                   
                 (HUMAN);, mRNA sequence. 
               
               
                 rxa00571 
                 1280 
                 GB_BA1: MTCY78 
                 33818 
                 Z77165 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 145/162. 
                 
                   Mycobacterium 
                 
                 38,468 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_PR3: AC005788 
                 36224 
                 AC005788 
                   Homo sapiens  chromosome 19, cosmid R26652, complete sequence. 
                 
                   Homo sapiens 
                 
                 36,911 
                 06-OCT-1998 
               
               
                   
                   
                 GB_PR3: AC005338 
                 34541 
                 AC005338 
                   Homo sapiens  chromosome 19, cosmid R31646, complete sequence. 
                 
                   Homo sapiens 
                 
                 36,911 
                 30-Jul-98 
               
               
                 rxa00590 
                 1288 
                 GB_HTG6: AC010932 
                 203273 
                 AC010932 
                   Homo sapiens  chromosome 15 clone RP11-296E22 map 15, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 37,242 
                 30-Nov-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 36 unordered pieces. 
               
               
                   
                   
                 GB_HTG6: AC010932 
                 203273 
                 AC010932 
                   Homo sapiens  chromosome 15 clone RP11-296E22 map 15, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 36,485 
                 30-Nov-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 36 unordered pieces. 
               
               
                   
                   
                 GB_BA1: MSGB26CS 
                 37040 
                 L78816 
                   Mycobacterium leprae  cosmid B26 DNA sequence. 
                 
                   Mycobacterium leprae 
                 
                 39,272 
                 15-Jun-96 
               
               
                 rxa00591 
                 1476 
                 GB_IN1: CEK09E9 
                 30098 
                 Z79602 
                   Caenorhabditis elegans  cosmid K09E9, complete sequence. 
                 
                   Caenorhabditis elegans 
                 
                 34,092 
                 2-Sep-99 
               
               
                   
                   
                 GB_PR4: AF135802 
                 4965 
                 AF135802 
                   Homo sapiens  thyroid hormone receptor-associated protein complex component 
                 
                   Homo sapiens 
                 
                 36,310 
                 9-Apr-99 
               
               
                   
                   
                   
                   
                   
                 TRAP170 mRNA, complete cds. 
               
               
                   
                   
                 GB_PR4: AF104256 
                 4365 
                 AF104256 
                   Homo sapiens  transcriptional co-activator CRSP150 (CRSP150) mRNA, complete 
                 
                   Homo sapiens 
                 
                 36,617 
                 4-Feb-99 
               
               
                   
                   
                   
                   
                   
                 cds. 
               
               
                 rxa00596 
                 576 
                 GB_PR3: AC004659 
                 129577 
                 AC004659 
                   Homo sapiens  chromosome 19, CIT-HSP-87m17 BAC clone, complete sequence. 
                 
                   Homo sapiens 
                 
                 34,321 
                 02-MAY-1998 
               
               
                   
                   
                 GB_PR3: AC004659 
                 129577 
                 AC004659 
                   Homo sapiens  chromosome 19, CIT-HSP-87m17 BAC clone, complete sequence. 
                 
                   Homo sapiens 
                 
                 35,739 
                 02-MAY-1998 
               
               
                   
                   
                 GB_PR1: HUMCBP2 
                 2047 
                 D83174 
                 Human mRNA for collagen binding protein 2, complete cds. 
                 
                   Homo sapiens 
                 
                 40,404 
                 6-Feb-99 
               
               
                 rxa00607 
                 504 
                 GB_BA1: MTV010 
                 3400 
                 AL021186 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 119/162. 
                 
                   Mycobacterium 
                 
                 40,862 
                 23-Jun-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: MTV010 
                 3400 
                 AL021186 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 119/162. 
                 
                   Mycobacterium 
                 
                 38,833 
                 23-Jun-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                 rxa00623 
                 1461 
                 GB_BA1: MTCY428 
                 26914 
                 Z81451 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 107/162. 
                 
                   Mycobacterium 
                 
                 60,552 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: RSPNGR234 
                 34010 
                 Z68203 
                   Rhizobium  sp. plasmid NGR234a DNA. 
                   Rhizobium  sp. 
                 51,992 
                 8-Aug-96 
               
               
                   
                   
                 GB_BA2: AE000101 
                 10057 
                 AE000101 
                   Rhizobium  sp, NGR234 plasmid pNGR234a, section 38 of 46 of the complete 
                   Rhizobium  sp. NGR234 
                 51,992 
                 12-DEC-1997 
               
               
                   
                   
                   
                   
                   
                 plasmid sequence. 
               
               
                 rxa00681 
               
               
                 rxa00690 
                 1269 
                 GB_HTG5: AC008338 
                 136685 
                 AC008338 
                   Drosophila melanogaster  chromosome X clone BACR30J04 (D908) RPCI-98 30.J.4 
                 
                   Drosophila melanogaster 
                 
                 35,341 
                 15-Nov-99 
               
               
                   
                   
                   
                   
                   
                 map 19C-19E strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 93 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC009766 
                 170502 
                 AC009766 
                   Homo sapiens  chromosome 11 clone 404_A_03 map 11, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 37,984 
                 19-OCT-1 999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 27 unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC009766 
                 170502 
                 AC009766 
                   Homo sapiens  chromosome 11 clone 404_A_03 map 11, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 37,984 
                 19-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 27 unordered pieces. 
               
               
                 rxa00733 
                 1008 
                 GB_EST30: AU054038 
                 245 
                 AU054038 
                 AU054038  Dictyostelium discoideum  SL ( H. Urushihara )  Dictyostelium discoideum   
                 
                   Dictyostelium discoideum 
                 
                 43,265 
                 28-Apr-99 
               
               
                   
                   
                   
                   
                   
                 cDNA clone SLK472, mRNA sequence. 
               
               
                   
                   
                 GB_EST30: AU054038 
                 245 
                 AU054038 
                 AU054038  Dictyostelium discoideum  SL ( H. Urushihara )  Dictyostelium discoideum   
                 
                   Dictyostelium discoideum 
                 
                 43,265 
                 28-Apr-99 
               
               
                   
                   
                   
                   
                   
                 cDNA clone SLK472, mRNA sequence. 
               
               
                 rxa00735 
                 692 
                 GB_BA1: MTCY50 
                 36030 
                 Z77137 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 55/162. 
                 
                   Mycobacterium 
                 
                 36,819 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: D90904 
                 150894 
                 D90904 
                   Synechocystis  sp. PCC6803 complete genome, 6/27, 630555-781448. 
                   Synechocystis  sp. 
                 52,585 
                 7-Feb-99 
               
               
                   
                   
                 GB_BA1: D90904 
                 150894 
                 D90904 
                   Synechocystis  sp. PCC6803 complete genome, 6/27, 630555-781448. 
                   Synechocystis  sp. 
                 39,699 
                 7-Feb-99 
               
               
                 rxa00796 
                 298 
                 GB_GSS14: AQ579838 
                 651 
                 AQ579838 
                 T135342b shotgun sub-library of BAC clone 31P06 Medicago truncatula genomic 
                 
                   Medicago truncatula 
                 
                 37,153 
                 27-Sep-99 
               
               
                   
                   
                   
                   
                   
                 clone 31-P-06-C-054, genomic survey sequence. 
               
               
                   
                   
                 GB_PR4: AC007625 
                 174701 
                 AC007625 
                 Genomic sequence of  Homo sapiens  clone 2314F2 from chromosome 18, complete 
                 
                   Homo sapiens 
                 
                 38,014 
                 30-Jun-99 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                   
                   
                 GB_EST14: AA427576 
                 580 
                 AA427576 
                 zw54b04.s1 Soares_total_fetus_Nb2HF8_9w  Homo sapiens  cDNA clone 
                 
                   Homo sapiens 
                 
                 42,731 
                 16-OCT-1997 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 773839 3′ similar to gb: M86852 PEROXISOME ASSEMBLY FACTOR-1 
               
               
                   
                   
                   
                   
                   
                 (HUMAN);, mRNA sequence. 
               
               
                 rxa00801 
                 756 
                 GB_BA1: MTV022 
                 13025 
                 AL021925 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 100/162. 
                 
                   Mycobacterium 
                 
                 59,350 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_RO: AC002109 
                 160048 
                 AC002109 
                 Genomic sequence from Mouse 9, complete sequence. 
                 
                   Mus musculus 
                 
                 39,398 
                 9-Sep-97 
               
               
                   
                   
                 GB_BA1: MTV022 
                 13025 
                 AL021925 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 100/162. 
                 
                   Mycobacterium 
                 
                 36,842 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                 rxa00802 
                 837 
                 GB_GSS14: AQ563349 
                 642 
                 AQ563349 
                 HS_5335_B2_A09_T7A RPCI-11 Human Male BAC Library  Homo   
                 
                   Homo sapiens 
                 
                 37,649 
                 29-MAY-1999 
               
               
                   
                   
                   
                   
                   
                   sapiens  genomic clone Plate = 911 Col = 18 Row = B, genomic survey sequence. 
               
               
                   
                   
                 GB_BA1: DIHCLPBA 
                 2441 
                 M32229 
                   B. nodosus  clpB gene encoding a regulatory subunit of ATP-dependent protease. 
                 
                   Dichelobacter nodosus 
                 
                 41,140 
                 26-Apr-93 
               
               
                   
                   
                 GB_GSS3: B61538 
                 698 
                 B61538 
                 T17M17TR TAMU  Arabidopsis thaliana  genomic clone T17M17, genomic survey 
                 
                   Arabidopsis thaliana 
                 
                 36,946 
                 21-Nov-97 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                 rxa00819 
                 1452 
                 GB_HTG3: AC008691_1 
                 110000 
                 AC008691 
                   Homo sapiens  chromosome 5 clone CIT978SKB_63A22, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 38,270 
                 3-Aug-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 253 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC008691_1 
                 110000 
                 AC008691 
                   Homo sapiens  chromosome 5 clone CIT978SKB_63A22, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 38,270 
                 3-Aug-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 253 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC009127 
                 186591 
                 AC009127 
                   Homo sapiens  chromosome 16 clone RPCI-11_498D10, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 38,947 
                 3-Aug-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 49 unordered pieces. 
               
               
                 rxa00821 
                 966 
                 GB_HTG1: HS32B1 
                 271488 
                 AL023693 
                   Homo sapiens  chromosome 6 clone RP1-32B1, *** SEQUENCING IN PROGRESS 
                 
                   Homo sapiens 
                 
                 36,565 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 ***, in unordered pieces. 
               
               
                   
                   
                 GB_HTG1: HS32B1 
                 271488 
                 AL023693 
                   Homo sapiens  chromosome 6 clone RP1-32B1, *** SEQUENCING IN PROGRESS 
                 
                   Homo sapiens 
                 
                 36,565 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 ***, in unordered pieces. 
               
               
                   
                   
                 GB_PR3: AC004919 
                 75547 
                 AC004919 
                   Homo sapiens  PAC clone DJ0895B23 from UL, complete sequence. 
                 
                   Homo sapiens 
                 
                 34,346 
                 19-Sep-98 
               
               
                 rxa00827 
                 876 
                 GB_EST6: W06539 
                 300 
                 W06539 
                 T2367 MVAT4 bloodstream form of serodeme WRATat1.1 Trypanosoma brucei 
                 
                   Trypanosoma brucei 
                 
                 40,000 
                 12-Aug-96 
               
               
                   
                   
                   
                   
                   
                 rhodesiense cDNA 5′, mRNA sequence. 
                 
                   rhodesiense 
                 
               
               
                   
                   
                 GB_PR4: AC008179 
                 181745 
                 AC008179 
                   Homo sapiens  clone NH0576F01, complete sequence. 
                 
                   Homo sapiens 
                 
                 35,903 
                 28-Sep-99 
               
               
                   
                   
                 GB_EST18: AA710415 
                 533 
                 AA710415 
                 vt53f08.r1 Barstead mouse irradiated colon MPLRB7  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 41,562 
                 24-DEC-1997 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 1166823 5′, mRNA sequence. 
               
               
                 rxa00842 
                 1323 
                 GB_PR2: AC002379 
                 118595 
                 AC002379 
                 Human BAC clone GS165I04 from 7q21, complete sequence. 
                 
                   Homo sapiens 
                 
                 36,321 
                 23-Jul-97 
               
               
                   
                   
                 GB_PR2: AC002379 
                 118595 
                 AC002379 
                 Human BAC clone GS165I04 from 7q21, complete sequence. 
                 
                   Homo sapiens 
                 
                 37,284 
                 23-Jul-97 
               
               
                   
                   
                 GB_IN1: CEF02D8 
                 31624 
                 Z78411 
                   Caenorhabditis elegans  cosmid F02D8, complete sequence. 
                 
                   Caenorhabditis elegans 
                 
                 38,163 
                 23-Nov-98 
               
               
                 rxa00847 
                 1572 
                 GB_OV: XELRDS38A 
                 1209 
                 L79915 
                   Xenopus laevis  rds/peripherin (rds38) mRNA, complete cds. 
                 
                   Xenopus laevis 
                 
                 36,044 
                 30-Jul-97 
               
               
                   
                   
                 GB_HTG4: AC007920 
                 234529 
                 AC007920 
                   Homo sapiens  chromosome 3q27 clone RPCI11-208N14, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 33,742 
                 21-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 51 unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC007920 
                 234529 
                 AC007920 
                   Homo sapiens  chromosome 3q27 clone RPCI11-208N14, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 33,742 
                 21-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 51 unordered pieces. 
               
               
                 rxa00851 
                 732 
                 GB_HTG2: AC004064 
                 185000 
                 AC004064 
                   Homo sapiens  chromosome 4, *** SEQUENCING IN PROGRESS ***, 10 unordered 
                 
                   Homo sapiens 
                 
                 39,833 
                 9-Jul-98 
               
               
                   
                   
                   
                   
                   
                 pieces. 
               
               
                   
                   
                 GB_HTG2: AC004064 
                 185000 
                 AC004064 
                   Homo sapiens  chromosome 4, *** SEQUENCING IN PROGRESS ***, 10 unordered 
                 
                   Homo sapiens 
                 
                 39,833 
                 9-Jul-98 
               
               
                   
                   
                   
                   
                   
                 pieces. 
               
               
                   
                   
                 GB_PR3: HSJ824F16 
                 139330 
                 AL050325 
                 Human DNA sequence from clone 824F16 on chromosome 20, complete sequence. 
                 
                   Homo sapiens 
                 
                 39,833 
                 23-Nov-99 
               
               
                 rxa00852 
                 813 
                 GB_HTG3: AC010120 
                 121582 
                 AC010120 
                   Drosophila melanogaster  chromosome 3 clone BACR22N13 (D1061) RPCI-98 
                 
                   Drosophila melanogaster 
                 
                 36,855 
                 24-Sep-99 
               
               
                   
                   
                   
                   
                   
                 22.N.13 map 96F-96F strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 83 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC010120 
                 121582 
                 AC010120 
                   Drosophila melanogaster  chromosome 3 clone BACR22N13 (D1061) RPCI-98 
                 
                   Drosophila melanogaster 
                 
                 36,855 
                 24-Sep-99 
               
               
                   
                   
                   
                   
                   
                 22.N.13 map 96F-96F strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 83 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_HTG2: AC006898 
                 299308 
                 AC006898 
                   Caenorhabditis elegans  clone Y73B6x, *** SEQUENCING IN PROGRESS ***, 9 
                 
                   Caenorhabditis elegans 
                 
                 36,768 
                 24-Feb-99 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa00856 
               
               
                 rxa00870 
                 1635 
                 GB_BA1: STMMSDA 
                 3986 
                 L48550 
                   Streptomyces coelicolor  methylmalonic acid semialdehyde dehydrogenase (msdA) 
                 
                   Streptomyces coelicolor 
                 
                 63,743 
                 09-MAY-1996 
               
               
                   
                   
                   
                   
                   
                 gene, complete cds. 
               
               
                   
                   
                 GB_PAT: I92043 
                 713 
                 I92043 
                 Sequence 10 from patent U.S. Pat. No. 5726299. 
                 Unknown. 
                 38,850 
                 01-DEC-1998 
               
               
                   
                   
                 GB_PAT: I78754 
                 713 
                 I78754 
                 Sequence 10 from patent U.S. Pat. No. 5693781. 
                 Unknown. 
                 38,850 
                 3-Apr-98 
               
               
                 rxa00875 
                 690 
                 GB_BA2: AF119715 
                 549 
                 AF119715 
                   Escherichia coli  isopentyl diphosphate isomerase (idi) gene, complete cds. 
                 
                   Escherichia coli 
                 
                 54,827 
                 22-Apr-99 
               
               
                   
                   
                 GB_BA2: AE000372 
                 12144 
                 AE000372 
                   Escherichia coli  K-12 MG1655 section 262 of 400 of the complete genome. 
                 
                   Escherichia coli 
                 
                 51,416 
                 12-Nov-98 
               
               
                   
                   
                 GB_BA1: ECU28375 
                 55175 
                 U28375 
                   Escherichia coli  K-12 genome; approximately 64 to 65 minutes. 
                 
                   Escherichia coli 
                 
                 51,416 
                 08-DEC-1995 
               
               
                 rxa00878 
                 1986 
                 GB_HTG2: AC007472 
                 114003 
                 AC007472 
                   Drosophila melanogaster  chromosome 2 clone BACR30D19 (D587) RPCI-98 
                 
                   Drosophila melanogaster 
                 
                 36,592 
                 2-Aug-99 
               
               
                   
                   
                   
                   
                   
                 30.D.19 map 49E-49F strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 79 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_HTG2: AC007472 
                 114003 
                 AC007472 
                   Drosophila melanogaster  chromosome 2 clone BACR30D19 (D587) RPCI-98 
                 
                   Drosophila melanogaster 
                 
                 36,592 
                 2-Aug-99 
               
               
                   
                   
                   
                   
                   
                 30.D.19 map 49E-49F strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 79 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_HTG2: AC006798 
                 207370 
                 AC006798 
                   Caenorhabditis elegans  clone Y51F8, *** SEQUENCING IN PROGRESS ***, 30 
                 
                   Caenorhabditis elegans 
                 
                 36,699 
                 25-Feb-99 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa00880 
                 1968 
                 GB_EST4: H22888 
                 468 
                 H22888 
                 ym54e12.r1 Soares infant brain 1NIB  Home sapiens  cDNA clone IMAGE: 52158 5′, 
                 
                   Homo sapiens 
                 
                 37,179 
                 6-Jul-95 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                   
                   
                 GB_GSS13: AQ426858 
                 516 
                 AQ426858 
                 CITBI-E1-2578F1.TF CITBI-E1  Home sapiens  genomic clone 2578F1, genomic 
                 
                   Homo sapiens 
                 
                 38,447 
                 24-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                   
                   
                 GB_PR1: AB002335 
                 6289 
                 AB002335 
                 Human mRNA for KIAA0337 gene, complete cds. 
                 
                   Homo sapiens 
                 
                 35,799 
                 13-Feb-99 
               
               
                 rxa00899 
                 1389 
                 GB_BA1: NGU58849 
                 2401 
                 U58849 
                   Neisseria gonorrhoeae  pilS6 silent pilus locus. 
                 
                   Neisseria gonorrhoeae 
                 
                 40,623 
                 20-Jun-96 
               
               
                   
                   
                 GB_BA1: PLPDHOS 
                 3119 
                 L06822 
                 Plasmid pSa (from  Escherichia coli ) dihydropteroate synthase gene, 3′ end. 
                 
                   Plasmid pSa 
                 
                 38,966 
                 20-MAR-1996 
               
               
                   
                   
                 GB_BA1: PDGINTORF 
                 6747 
                 L06418 
                 Integron In7 (from Plasmid pDGO100 from  Escherichia coli ) integrase (int), 
                 
                   Plasmid pDGO100 
                 
                 38,966 
                 20-MAR-1996 
               
               
                   
                   
                   
                   
                   
                 aminoglycoside adenylyltransferase (aad), quaternary ammonium compound- 
               
               
                   
                   
                   
                   
                   
                 resistance protein, dihydrofolate reductase (dhfrX), and dihydropteroate 
               
               
                   
                   
                   
                   
                   
                 synthase (sull) genes. 
               
               
                 rxa00902 
                 1333 
                 GB_GSS15: AQ606873 
                 581 
                 AQ606873 
                 HS_5404_B2_H05_T7A RPCI-11 Human Male BAC Library  Homo sapiens   
                 
                   Homo sapiens 
                 
                 37,900 
                 10-Jun-99 
               
               
                   
                   
                   
                   
                   
                 genomic clone Plate = 980 Col = 10 Row = P, genomic survey sequence. 
               
               
                   
                   
                 GB_GSS9: AQ163442 
                 658 
                 AQ163442 
                 nbxb0007A07f CUGI Rice BAC Library  Oryza sativa  genomic clone nbxb0007A07f, 
                 
                   Oryza sativa 
                 
                 41,885 
                 12-Sep-98 
               
               
                   
                   
                   
                   
                   
                 genomic survey sequence. 
               
               
                   
                   
                 GB_PL1: PSST70 
                 4974 
                 X69213 
                   P. sativum  Psst70 gene for heat-shock protein. 
                 
                   Pisum sativum 
                 
                 36,866 
                 3-Jul-96 
               
               
                 rxa00931 
                 969 
                 GB_GSS1: FR0025208 
                 612 
                 AL018047 
                   F. rubripes  GSS sequence, clone 145D10aA8, genomic survey sequence. 
                 
                   Fugu rubripes 
                 
                 37,815 
                 10-DEC-1997 
               
               
                   
                   
                 GB_GSS1: FR0021844 
                 252 
                 AL014715 
                   F. rubripes  GSS sequence, clone 069K22aG5, genomic survey sequence. 
                 
                   Fugu rubripes 
                 
                 37,698 
                 10-DEC-1997 
               
               
                   
                   
                 GB_GSS12: AQ403344 
                 593 
                 AQ403344 
                 HS_2257_B1_B03_T7C CIT Approved Human Genomic Sperm Library D Homo 
                 
                   Homo sapiens 
                 
                 31,552 
                 13-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 sapiens genomic clone Plate = 2257 Col = 5 Row = D, genomic survey sequence. 
               
               
                 rxa00941 
                 1440 
                 GB_BA1: MTCY180 
                 44201 
                 Z97193 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 85/162. 
                 
                   Mycobacterium 
                 
                 37,902 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: MTCY180 
                 44201 
                 Z97193 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 85/162. 
                 
                   Mycobacterium 
                 
                 39,140 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA2: MSGKATG 
                 1745 
                 L14268 
                   Mycobacterium tuberculosis  ethyl methane sulphonate resistance protein (katG) 
                 
                   Mycobacterium 
                 
                 42,517 
                 26-Aug-99 
               
               
                   
                   
                   
                   
                   
                 gene, 3′end. 
                 
                   tuberculosis 
                 
               
               
                 rxa00962 
                 689 
                 GB_HTG6: AC010998 
                 144338 
                 AC010998 
                   Homo sapiens  clone RP11-95I16, *** SEQUENCING IN PROGRESS ***, 17 
                 
                   Homo sapiens 
                 
                 39,497 
                 08-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_GSS1: GGA340111 
                 990 
                 AJ232089 
                   Gallus gallus  anonymous sequence from Cosmid mapping to chromosome 2 
                 
                   Gallus gallus 
                 
                 37,970 
                 25-Aug-98 
               
               
                   
                   
                   
                   
                   
                 (Cosmid 34 - Contig 15), genomic survey sequence. 
               
               
                   
                   
                 GB_HTG6: AC010998 
                 144338 
                 AC010998 
                   Homo sapiens  clone RP11-95I16, *** SEQUENCING IN PROGRESS ***, 17 
                 
                   Homo sapiens 
                 
                 38,226 
                 08-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa01060 
                 1047 
                 GB_BA1: ECTTN7 
                 2280 
                 AJ001816 
                   Escherichia coli  left end of transposon Tn7 including type 2 Integron. 
                 
                   Escherichia coli 
                 
                 38,822 
                 4-Nov-97 
               
               
                   
                   
                 GB_IN2: AF176377 
                 8220 
                 AF176377 
                   Caenorhabditis briggsae  CES-1 (ces-1) gene, complete cds; and CPN-1 (cpn-1) 
                 
                   Caenorhabditis briggsae 
                 
                 39,921 
                 09-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 gene, partial cds. 
               
               
                   
                   
                 GB_GSS10: AQ196728 
                 429 
                 AQ196728 
                 CIT-HSP-2381F4.TR CIT-HSP  Homo sapiens  genomic clone 2381F4, genomic 
                 
                   Homo sapiens 
                 
                 39,019 
                 16-Sep-98 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                 rxa01067 
                 852 
                 GB_BA1: U00016 
                 42931 
                 U00016 
                   Mycobacterium leprae  cosmid B1937. 
                 
                   Mycobacterium leprae 
                 
                 58,303 
                 01-MAR-1994 
               
               
                   
                   
                 GB_BA1: SYCGROESL 
                 3256 
                 D12677 
                   Synechocystis  sp. groES and groEL genes. 
                   Synechocystis  sp. 
                 34,593 
                 3-Feb-99 
               
               
                   
                   
                 GB_BA1: D90905 
                 139467 
                 D90905 
                   Synechocystis  sp. PCC6803 complete genome, 7/27, 781449-920915. 
                   Synechocystis  sp. 
                 34,593 
                 7-Feb-99 
               
               
                 rxa01114 
                 1347 
                 GB_BA1: PSEFAOAB 
                 3480 
                 D10390 
                   P. fragi  faoA and faoB genes, complete cds. 
                 
                   Pseudomonas fragi 
                 
                 51,919 
                 2-Feb-99 
               
               
                   
                   
                 GB_BA1: AB014757 
                 6057 
                 AB014757 
                   Pseudomonas  sp. 61-3 genes for PhbR, acetoacetyl-CoA reductase, beta- 
                   Pseudomonas  sp. 61-3 
                 50,573 
                 26-DEC-1998 
               
               
                   
                   
                   
                   
                   
                 ketothiolase and PHB synthase, complete cds. 
               
               
                   
                   
                 GB_BA1: SC8D9 
                 38681 
                 AL035569 
                   Streptomyces coelicolor  cosmid 8D9. 
                 
                   Streptomyces coelicolor 
                 
                 42,200 
                 26-Feb-99 
               
               
                 rxa01136 
                 555 
                 GB_EST11: AA244557 
                 379 
                 AA244557 
                 mx07a01.r1 Soares mouse NML  Mus musculus  cDNA clone IMAGE: 679464 5′, 
                 
                   Mus musculus 
                 
                 39,050 
                 10-MAR-1997 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                   
                   
                 GB_EST14: AA407673 
                 306 
                 AA407673 
                 EST01834 Mouse 7.5 dpc embryo ectoplacental cone cDNA library  Mus musculus   
                 
                   Mus musculus 
                 
                 38,562 
                 26-Aug-98 
               
               
                   
                   
                   
                   
                   
                 cDNA clone C0014F02 3′, mRNA sequence. 
               
               
                   
                   
                 GB_EST26: AI390328 
                 604 
                 AI390328 
                 mx07a01.y1 Soares mouse NML  Mus musculus  cDNA clone IMAGE: 679464 5′, 
                 
                   Mus musculus 
                 
                 33,136 
                 2-Feb-99 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                 rxa01138 
                 540 
                 GB_OV: XLXINT1 
                 1278 
                 X13138 
                   Xenopus laevis  int-1 mRNA for int-1 protein. 
                 
                   Xenopus laevis 
                 
                 40,038 
                 31-MAR-1995 
               
               
                   
                   
                 GB_PR4: AC006054 
                 143738 
                 AC006054 
                   Homo sapiens  Xq28 BAC RPCI11-382P7 (Roswell Park Cancer Institute Human 
                 
                   Homo sapiens 
                 
                 37,996 
                 1-Apr-99 
               
               
                   
                   
                   
                   
                   
                 BAC Library) complete sequence. 
               
               
                   
                   
                 GB_PR4: AC006054 
                 143738 
                 AC006054 
                   Homo sapiens  Xq28 BAC RPCI11-382P7 (Roswell Park Cancer Institute Human 
                 
                   Homo sapiens 
                 
                 36,053 
                 1-Apr-99 
               
               
                   
                   
                   
                   
                   
                 BAC Library) complete sequence. 
               
               
                 rxa01172 
                 1578 
                 GB_BA1: SCE39 
                 23550 
                 AL049573 
                   Streptomyces coelicolor  cosmid E39. 
                 
                   Streptomyces coelicolor 
                 
                 62,357 
                 31-MAR-1999 
               
               
                   
                   
                 GB_BA1: MSU50335 
                 5193 
                 U50335 
                   Mycobacterium smegmatis  phage resistance (mpr) gene, complete cds. 
                 
                   Mycobacterium 
                 
                 37,853 
                 1-Feb-97 
               
               
                   
                   
                   
                   
                   
                   
                 
                   smegmatis 
                 
               
               
                   
                   
                 GB_BA1: BACTHRTRNA 
                 15467 
                 D84213 
                   Bacillus subtilis  genome, trnl-feuABC region. 
                 
                   Bacillus subtilis 
                 
                 53,807 
                 6-Feb-99 
               
               
                 rxa01191 
                 1713 
                 GB_PR2: HS1191B2 
                 60828 
                 AL022237 
                 Human DNA sequence from clone 1191B2 on chromosome 22q13.2-13.3. Contains 
                 
                   Homo sapiens 
                 
                 38,366 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 part of the BIK (NBK, BP4, BIP1) gene for BCL2-interacting killer (apoptosis- 
               
               
                   
                   
                   
                   
                   
                 inducing), a 40S Ribososmal Protein S25 pseudogene and part of an 
               
               
                   
                   
                   
                   
                   
                 alternatively spliced novel Acyl Transferase gene similar to  C. elegans  C50D2.7. 
               
               
                   
                   
                   
                   
                   
                 Contains ESTs, STSs, GSSs, two putative CpG islands and genomic marker 
               
               
                   
                   
                   
                   
                   
                 D22S1151, complete sequence. 
               
               
                   
                   
                 GB_PR2: HS1191B2 
                 60828 
                 AL022237 
                 Human DNA sequence from clone 1191B2 on chromosome 22q13.2-13.3. Contains 
                 
                   Homo sapiens 
                 
                 39,595 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 part of the BIK (NBK, BP4, BIP1) gene for BCL2-interacting killer (apoptosis- 
               
               
                   
                   
                   
                   
                   
                 inducing), a 40S Ribososmal Protein S25 pseudogene and part of an 
               
               
                   
                   
                   
                   
                   
                 alternatively spliced novel Acyl Transferase gene similar to  C. elegans  C50D2.7. 
               
               
                   
                   
                   
                   
                   
                 Contains ESTs, STSs, GSSs, two putative CpG islands and genomic marker 
               
               
                   
                   
                   
                   
                   
                 D22S1151, complete sequence. 
               
               
                 rxa01205 
                 554 
                 GB_BA1: MTCY373 
                 35516 
                 Z73419 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 57/162. 
                 
                   Mycobacterium 
                 
                 57,762 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_PL1: ATY12776 
                 38483 
                 Y12776 
                   Arabidopsis thaliana  DNA, 40 kb surrounding ACS1 locus. 
                 
                   Arabidopsis thaliana 
                 
                 32,971 
                 7-Sep-98 
               
               
                   
                   
                 GB_PL2: ATT6K21 
                 99643 
                 AL021889 
                   Arabidopsis thaliana  DNA chromosome 4, BAC clone T6K21 (ESSA project). 
                 
                   Arabidopsis thaliana 
                 
                 35,273 
                 16-Aug-99 
               
               
                 rxa01212 
                 1047 
                 GB_BA2: SCD25 
                 41622 
                 AL118514 
                   Streptomyces coelicolor  cosmid D25. 
                 
                   Streptomyces coelicolor 
                 
                 39,654 
                 21-Sep-99 
               
               
                   
                   
                   
                   
                   
                   
                 A3(2) 
               
               
                   
                   
                 GB_BA1: SLGLYUB 
                 2576 
                 X65556 
                   S. lividans  tRNA-GlyU beta gene. 
                 
                   Streptomyces lividans 
                 
                 54,493 
                 20-DEC-1993 
               
               
                   
                   
                 GB_BA1: SCH10 
                 39524 
                 AL049754 
                   Streptomyces coelicolor  cosmid H10. 
                 
                   Streptomyces coelicolor 
                 
                 44,638 
                 04-MAY-1999 
               
               
                 rxa01219 
                 1005 
                 GB_PAT: A68024 
                 520 
                 A68024 
                 Sequence 19 from Patent WO9743409. 
                 unidentified 
                 42,553 
                 05-MAY-1999 
               
               
                   
                   
                 GB_PAT: A68025 
                 193 
                 A68025 
                 Sequence 20 from Patent WO9743409. 
                 unidentified 
                 43,229 
                 05-MAY-1999 
               
               
                   
                   
                 GB_PAT: A68027 
                 193 
                 A68027 
                 Sequence 22 from Patent WO9743409. 
                 unidentified 
                 38,342 
                 05-MAY-1999 
               
               
                 rxa01220 
                 1200 
                 GB_PR3: HS512B11 
                 64356 
                 AL031058 
                 Human DNA sequence from clone 512B11 on chromosome 6p24-25. Contains the 
                 
                   Homo sapiens 
                 
                 35,478 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 Desmoplakin I (DPI) gene, ESTs, STSs and GSSs, complete sequence. 
               
               
                   
                   
                 GB_EST6: N99239 
                 424 
                 N99239 
                 zb76h11.s1 Soares_senescent_fibroblasts_NbHSF  Homo sapiens  cDNA clone 
                 
                   Homo sapiens 
                 
                 39,623 
                 20-Aug-96 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 309573 3′, mRNA sequence. 
               
               
                   
                   
                 GB_EST16: AA554268 
                 400 
                 AA554268 
                 nk36c09.s1 NCI_CGAP_GC2  Homo sapiens  cDNA clone IMAGE: 1015600 
                 
                   Homo sapiens 
                 
                 36,111 
                 8-Sep-97 
               
               
                   
                   
                   
                   
                   
                 3′ similar to gb: X01677 GLYCERALDEHYDE 3-PHOSPHATE 
               
               
                   
                   
                   
                   
                   
                 DEHYDROGENASE, LIVER (HUMAN);, mRNA sequence. 
               
               
                 rxa01221 
                 849 
                 GB_PR4: AF179633 
                 96371 
                 AF179633 
                   Homo sapiens  chromosome 16 map 16q23.3-q24.1 sequence. 
                 
                   Homo sapiens 
                 
                 40,199 
                 5-Sep-99 
               
               
                   
                   
                 GB_VI: EHVU20824 
                 184427 
                 U20824 
                 Equine herpesvirus 2, complete genome. 
                 Equine herpesvirus 2 
                 37,001 
                 2-Feb-96 
               
               
                   
                   
                 GB_BA2: AE000407 
                 10601 
                 AE000407 
                   Escherichia coli  K-12 MG1655 section 297 of 400 of the complete genome. 
                 
                   Escherichia coli 
                 
                 39,471 
                 12-Nov-98 
               
               
                 rxa01222 
                 822 
                 GB_PAT: AR068625 
                 28804 
                 AR068625 
                 Sequence 1 from patent U.S. Pat. No. 5854034. 
                 Unknown. 
                 40,574 
                 29-Sep-99 
               
               
                   
                   
                 GB_BA2: SSU51197 
                 28804 
                 U51197 
                   Sphingomonas  S88 sphingan polysaccharide synthesis (spsG), (spsS), (spsR), 
                   Sphingomonas  sp. S88 
                 40,574 
                 16-MAY-1996 
               
               
                   
                   
                   
                   
                   
                 glycosyl transferase (spsQ), (spsl), glycosyl transferase (spsK), glycosyl transferase 
               
               
                   
                   
                   
                   
                   
                 (spsL), (spsJ), (spsF), (spsD), (spsC), (spsE), Urf 32, Urf 26, ATP-binding 
               
               
                   
                   
                   
                   
                   
                 cassette transporter (atrD), ATP-binding cassette transporter (atrB), glucosyl- 
               
               
                   
                   
                   
                   
                   
                 isoprenylphosphate transferase (spsB), glucose-1-phosphate thymidylyltransferase 
               
               
                   
                   
                   
                   
                   
                 (rhsA), dTDP-6-deoxy-D-glucose-3,5-epimerase (rhsC) dTDP-D-glucose-4,6- 
               
               
                   
                   
                   
                   
                   
                 dehydratase (rhsB), dTDP-6-deoxy-L-mannose-dehydrogenase (rhsD), Urf 31, and 
               
               
                   
                   
                   
                   
                   
                 Urf 34 genes, complete cds. 
               
               
                   
                   
                 GB_IN1: BBU44918 
                 2791 
                 U44918 
                   Babesia bovis  ATP-binding protein (babc) mRNA, complete cds. 
                 
                   Babesia bovis 
                 
                 39,228 
                 9-Aug-97 
               
               
                 rxa01260 
                 1305 
                 GB_BA1: CGLPD 
                 1800 
                 Y16642 
                   Corynebacterium glutamicum  lpd gene, complete CDS. 
                 
                   Corynebacterium 
                 
                 99,923 
                 1-Feb-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: MTV038 
                 16094 
                 AL021933 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 24/162. 
                 
                   Mycobacterium 
                 
                 59,056 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_PR3: AC005618 
                 176714 
                 AC005618 
                   Homo sapiens  chromosome 5, BAC clone 249h5 (LBNL H149), complete sequence. 
                 
                   Homo sapiens 
                 
                 36,270 
                 5-Sep-98 
               
               
                 rxa01261 
                 294 
                 GB_BA1: CGLPD 
                 1800 
                 Y16642 
                   Corynebacterium glutamicum  lpd gene, complete CDS. 
                 
                   Corynebacterium 
                 
                 100,000 
                 1-Feb-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_HTG4: AC010045 
                 164829 
                 AC010045 
                   Drosophila melanogaster  chromosome 3L/75A1 clone RPCI98-17C17, *** 
                 
                   Drosophila melanogaster 
                 
                 50,512 
                 16-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 SEQUENCING IN PROGRESS ***, 50 unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC010045 
                 164829 
                 AC010045 
                   Drosophila melanogaster  chromosome 3L/75A1 clone RPCI98-17C17, *** 
                 
                   Drosophila melanogaster 
                 
                 50,512 
                 16-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 SEQUENCING IN PROGRESS ***, 50 unordered pieces. 
               
               
                 rxa01269 
                 564 
                 GB_BA2: AF125164 
                 26443 
                 AF125164 
                   Bacteroides fragilis  638R polysaccharide B (PS B2) biosynthesis locus, complete 
                 
                   Bacteroides fragilis 
                 
                 56,071 
                 01-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 sequence; and unknown genes. 
               
               
                   
                   
                 GB_BA1: AB002668 
                 24907 
                 AB002668 
                   Actinobacillus actinomycetemcomitans  DNA for glycosyltransferase, lytic 
                 
                   Actinobacillus 
                 
                 46,679 
                 21-Feb-98 
               
               
                   
                   
                   
                   
                   
                 transglycosylase, dTDP-4-rhamnose reductase, complete cds. 
                 
                   actinomycetemcomitans 
                 
               
               
                   
                   
                 GB_BA1: AB010415 
                 23112 
                 AB010415 
                   Actinobacillus actinomycetemcomitans  gene cluster for 6-deoxy-L-talan synthesis, 
                 
                   Actinobacillus 
                 
                 46,679 
                 13-Feb-99 
               
               
                   
                   
                   
                   
                   
                 complete cds. 
                 
                   actinomycetemcomitans 
                 
               
               
                 rxa01291 
                 1056 
                 GB_STS: AU027820 
                 238 
                 AU027820 
                   Rattus norvegicus , OTSUKA clone, OT78.02/918b07, microsatellite sequence, 
                 
                   Rattus norvegicus 
                 
                 34,874 
                 02-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 sequence tagged site. 
               
               
                   
                   
                 GB_STS: AU027820 
                 238 
                 AU027820 
                   Rattus norvegicus , OTSUKA clone, OT78.02/918b07, microsatellite sequence, 
                 
                   Rattus norvegicus 
                 
                 34,874 
                 02-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 sequence tagged site. 
               
               
                   
                   
                 GB_HTG3: AC006445 
                 174547 
                 AC006445 
                   Homo sapiens  chromosome 4, *** SEQUENCING IN PROGRESS ***, 7 unordered 
                 
                   Homo sapiens 
                 
                 34,812 
                 15-Sep-99 
               
               
                   
                   
                   
                   
                   
                 pieces. 
               
               
                 rxa01292 
                 1308 
                 GB_BA1: BSUB0017 
                 217420 
                 Z99120 
                   Bacillus subtilis  complete genome (section 17 of 21): from 3197001 to 3414420. 
                 
                   Bacillus subtilis 
                 
                 37,802 
                 26-Nov-97 
               
               
                   
                   
                 GB_HTG3: AC010580 
                 121119 
                 AC010580 
                   Drosophila melanogaster  chromosome 3 clone BACR48J06 (D1102) RPCI-98 48.J.6 
                 
                   Drosophila melanogaster 
                 
                 35,637 
                 01-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 map 96F-96F strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 71 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC010580 
                 121119 
                 AC010580 
                   Drosophila melanogaster  chromosome 3 clone BACR48J06 (D1102) RPCI-98 48.J.6 
                 
                   Drosophila melanogaster 
                 
                 35,637 
                 01-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 map 96F-96F strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 71 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa01293 
                 450 
                 GB_GSS8: AQ001809 
                 705 
                 AQ001809 
                 CIT-HSP-2290D17.TF CIT-HSP  Homo sapiens  genomic clone 2290D17, genomic 
                 
                   Homo sapiens 
                 
                 42,021 
                 26-Jun-98 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                   
                   
                 GB_GSS8: AQ001809 
                 705 
                 AQ001809 
                 CIT-HSP-2290D17.TF CIT-HSP  Homo sapiens  genomic clone 2290D17, genomic 
                 
                   Homo sapiens 
                 
                 40,323 
                 26-Jun-98 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                 rxa01339 
                 1111 
                 GB_PL1: MGU60290 
                 4614 
                 U60290 
                   Magnaporthe grisea  nitrogen regulatory protein (NUT1) gene, complete cds. 
                 
                   Magnaporthe grisea 
                 
                 38,707 
                 3-Jul-96 
               
               
                   
                   
                 GB_HTG3: AC011371 
                 189187 
                 AC011371 
                   Homo sapiens  chromosome 5 clone CIT978SKB_107C20, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 39,741 
                 06-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 31 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC011371 
                 189187 
                 AC011371 
                   Homo sapiens  chromosome 5 clone CIT978SKB_107C20, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 39,741 
                 06-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 31 unordered pieces. 
               
               
                 rxa01382 
                 1192 
                 GB_HTG4: AC009892 
                 138122 
                 AC009892 
                   Homo sapiens  chromosome 19 clone CIT978SKB_83J4, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 40,154 
                 31-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 6 ordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC009892 
                 138122 
                 AC009892 
                   Homo sapiens  chromosome 19 clone CIT978SKB_83J4, SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 40,154 
                 31-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 6 ordered pieces. 
               
               
                   
                   
                 GB_PR3: AC002416 
                 128915 
                 AC002416 
                 Human Chromosome X, complete sequence. 
                 
                   Homo sapiens 
                 
                 37,521 
                 29-Jan-98 
               
               
                 rxa01399 
                 1142 
                 GB_EST9: AA096601 
                 524 
                 AA096601 
                 mo03b09.r1 Stratagene mouse lung 937302  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 40,525 
                 15-Feb-97 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 552473 5′ similar to gb: L06505 60S RIBOSOMAL PROTEIN L12 
               
               
                   
                   
                   
                   
                   
                 (HUMAN); gb: L04280  Mus musculus  ribosomal protein (MOUSE);, 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                   
                   
                 GB_EST37: AI982114 
                 626 
                 AI982114 
                 pat.pk0074.e9.f chicken activated T cell cDNA  Gallus gallus  cDNA clone 
                 
                   Gallus gallus 
                 
                 37,785 
                 15-Sep-99 
               
               
                   
                   
                   
                   
                   
                 pat.pk0074.e9.f 5′ similar to H-ATPase B subunit, mRNA sequence. 
               
               
                   
                   
                 GB_OV: GGU20766 
                 1645 
                 U20766 
                   Gallus gallus  vacuolar H+-ATPase B subunit gene, complete cds. 
                 
                   Gallus gallus 
                 
                 38,244 
                 07-DEC-1995 
               
               
                 rxa01420 
                 1065 
                 GB_HTG2: AC005690 
                 193424 
                 AC005690 
                   Homo sapiens  chromosome 4, *** SEQUENCING IN PROGRESS ***, 7 unordered 
                 
                   Homo sapiens 
                 
                 37,464 
                 11-Apr-99 
               
               
                   
                   
                   
                   
                   
                 pieces. 
               
               
                   
                   
                 GB_HTG2: AC005690 
                 193424 
                 AC005690 
                   Homo sapiens  chromosome 4, *** SEQUENCING IN PROGRESS ***, 7 unordered 
                 
                   Homo sapiens 
                 
                 37,464 
                 11-Apr-99 
               
               
                   
                   
                   
                   
                   
                 pieces. 
               
               
                   
                   
                 GB_HTG2: AC006637 
                 22092 
                 AC006637 
                   Caenorhabditis elegans  clone F41B4, *** SEQUENCING IN PROGRESS ***, 1 
                 
                   Caenorhabditis elegans 
                 
                 37,488 
                 23-Feb-99 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa01467 
                 414 
                 GB_HTG1: CEY102G3_21 
                 10000 
                 AL020985 
                   Caenorhabditis elegans  chromosome V clone Y102G3, *** SEQUENCING IN 
                 
                   Caenorhabditis elegans 
                 
                 35,437 
                 3-Dec-98 
               
               
                   
                   
                 GB_HTG1: CEY102G3_21 
                 10000 
                 AL020985 
                   Caenorhabditis elegans  chromosome V clone Y102G3, *** SEQUENCING IN 
                 
                   Caenorhabditis elegans 
                 
                 35,437 
                 3-Dec-98 
               
               
                   
                   
                 GB_HTG1: CEY113G7_41 
                 10000 
                 AL031113 
                   Caenorhabditis elegans  chromosome V clone Y113G7, *** SEQUENCING IN 
                 
                   Caenorhabditis elegans 
                 
                 35,437 
                 12-Jan-99 
               
               
                 rxa01576 
                 882 
                 GB_BA2: AF030975 
                 2511 
                 AF030975 
                   Aeromonas salmonicida  chaperonin GroES and chaperonin GroEL genes, complete 
                 
                   Aeromonas salmonicida 
                 
                 41,516 
                 2-Apr-98 
               
               
                   
                   
                   
                   
                   
                 cds. 
               
               
                   
                   
                 GB_BA2: AF030975 
                 2511 
                 AF030975 
                   Aeromonas salmonicida  chaperonin GroES and chaperonin GroEL genes, complete 
                 
                   Aeromonas salmonicida 
                 
                 38,171 
                 2-Apr-98 
               
               
                   
                   
                   
                   
                   
                 cds. 
               
               
                   
                   
                 GB_EST22: AI068560 
                 965 
                 AI068560 
                 mgae0003aC11f  Magnaporthe grisea  Appressorium Stage cDNA Library Pyricularia 
                 
                   Pyricularia grisea 
                 
                 40,073 
                 09-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 grisea cDNA clone mgae0003aC11f5′, mRNA sequence. 
               
               
                 rxa01580 
                 840 
                 GB_GSS14: AQ554460 
                 681 
                 AQ554460 
                 RPCI-11-419F2.TV RPCI-11  Homo sapiens  genomic clone RPCI-11-419F2, genomic 
                 
                   Homo sapiens 
                 
                 36,522 
                 28-MAY-1999 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                   
                   
                 GB_IN2: AC005449 
                 85518 
                 AC005449 
                   Drosophila melanogaster , chromosome 2R, region 44C4-44C5, P1 clone DS06765, 
                 
                   Drosophila melanogaster 
                 
                 36,609 
                 23-DEC-1998 
               
               
                   
                   
                   
                   
                   
                 complete sequence. 
               
               
                   
                   
                 GB_IN2: AC005449 
                 85518 
                 AC005449 
                   Drosophila melanogaster , chromosome 2R, region 44C4-44C5, P1 clone DS06765, 
                 
                   Drosophila melanogaster 
                 
                 33,612 
                 23-DEC-1998 
               
               
                   
                   
                   
                   
                   
                 complete sequence. 
               
               
                 rxa01584 
               
               
                 rxa01604 
                 771 
                 GB_HTG3: AC011352 
                 160167 
                 AC011352 
                   Homo sapiens  chromosome 5 clone CIT-HSPC_327F10, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 33,688 
                 06-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 15 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC011352 
                 160167 
                 AC011352 
                   Homo sapiens  chromosome 5 clone CIT-HSPC_327F10, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 33,688 
                 06-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 15 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC011402 
                 168868 
                 AC011402 
                   Homo sapiens  chromosome 5 clone CIT978SKB_38B5, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 33,688 
                 06-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 7 unordered pieces. 
               
               
                 rxa01614 
                 1146 
                 GB_BA1: CGA224946 
                 2408 
                 AJ224946 
                   Corynebacterium glutamicum  DNA for L-Malate:quinone oxidoreductase. 
                 
                   Corynebacterium 
                 
                 42,284 
                 11-Aug-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_EST17: AA608825 
                 439 
                 AA608825 
                 af03g07.s1 Soares_testis_NHT  Homo sapiens  cDNA clone IMAGE: 1030620 3′ 
                 
                   Homo sapiens 
                 
                 40,092 
                 02-MAR-1998 
               
               
                   
                   
                   
                   
                   
                 similar to TR: G976083 G976083 HISTONE H2A RELATED.;, mRNA sequence. 
               
               
                   
                   
                 GB_PR4: AC005377 
                 102311 
                 AC005377 
                   Homo sapiens  PAC clone DJ1136G02 from 7q32-q34, complete sequence. 
                 
                   Homo sapiens 
                 
                 37,811 
                 28-Apr-99 
               
               
                 rxa01629 
                 1635 
                 GB_BA1: CGPROPGEN 
                 2936 
                 Y12537 
                   C. glutamicum  proP gene. 
                 
                   Corynebacterium 
                 
                 100,000 
                 17-Nov-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: CGPROPGEN 
                 2936 
                 Y12537 
                   C. glutamicum  proP gene. 
                 
                   Corynebacterium 
                 
                 100,000 
                 17-Nov-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_PR4: AF191071 
                 88481 
                 AF191071 
                   Homo sapiens  chromosome 8 clone BAC 388D06, complete sequence. 
                 
                   Homo sapiens 
                 
                 35,612 
                 11-OCT-1999 
               
               
                 rxa01644 
                 1401 
                 GB_BA1: MSGB577COS 
                 37770 
                 L01263 
                   M. leprae  genomic dna sequence, cosmid b577. 
                 
                   Mycobacterium leprae 
                 
                 55,604 
                 14-Jun-96 
               
               
                   
                   
                 GB_BA1: MLCB2407 
                 35615 
                 AL023596 
                   Mycobacterium leprae  cosmid B2407. 
                 
                   Mycobacterium leprae 
                 
                 36,416 
                 27-Aug-99 
               
               
                   
                   
                 GB_BA1: MTV025 
                 121125 
                 AL022121 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 155/162. 
                 
                   Mycobacterium 
                 
                 55,844 
                 24-Jun-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                 rxa01667 
                 1329 
                 GB_BA1: CGU43536 
                 3464 
                 U43536 
                   Corynebacterium glutamicum  heat shock, ATP-binding protein (clpB) gene, complete 
                 
                   Corynebacterium 
                 
                 100,000 
                 13-MAR-1997 
               
               
                   
                   
                   
                   
                   
                 cds. 
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_HTG4: AC009841 
                 164434 
                 AC009841 
                   Drosophila melanogaster  chromosome 3L/77E1 clone RPCI98-13F11, *** 
                 
                   Drosophila melanogaster 
                 
                 33,205 
                 16-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 SEQUENCING IN PROGRESS ***, 70 unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC009841 
                 164434 
                 AC009841 
                   Drosophila melanogaster  chromosome 3L/77E1 clone RPCI98-13F11, *** 
                 
                   Drosophila melanogaster 
                 
                 33,205 
                 16-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 SEQUENCING IN PROGRESS ***, 70 unordered pieces. 
               
               
                 rxa01722 
                 1848 
                 GB_GSS1: FR0022586 
                 522 
                 AL015452 
                   F. rubripes  GSS sequence, clone 077P23aB10, genomic survey sequence. 
                 
                   Fugu rubripes 
                 
                 40,192 
                 10-DEC-1997 
               
               
                   
                   
                 GB_GSS1: FR0022584 
                 485 
                 AL015450 
                   F. rubripes  GSS sequence, clone 077P23aB11, genomic survey sequence. 
                 
                   Fugu rubripes 
                 
                 35,876 
                 10-DEC-1997 
               
               
                   
                   
                 GB_IN1: CET26H2 
                 37569 
                 Z82055 
                   Caenorhabditis elegans  cosmid T26H2, complete sequence. 
                 
                   Caenorhabditis elegans 
                 
                 34,759 
                 19-Nov-99 
               
               
                 rxa01727 
                 1401 
                 GB_BA2: CORCSLYS 
                 2821 
                 M89931 
                   Corynebacterium glutamicum  beta C-S lyase (aecD) and branched-chain amino acid 
                 
                   Corynebacterium 
                 
                 99,929 
                 4-Jun-98 
               
               
                   
                   
                   
                   
                   
                 uptake carrier (brnQ) genes, complete cds, and hypothetical protein Yhbw (yhbw) 
                 
                   glutamicum 
                 
               
               
                   
                   
                   
                   
                   
                 gene, partial cds. 
               
               
                   
                   
                 GB_HTG6: AC011037 
                 167849 
                 AC011037 
                   Homo sapiens  clone RP11-7F18, WORKING DRAFT SEQUENCE, 19 unordered 
                 
                   Homo sapiens 
                 
                 36,903 
                 30-Nov-99 
               
               
                   
                   
                   
                   
                   
                 pieces. 
               
               
                   
                   
                 GB_HTG6: AC011037 
                 167849 
                 AC011037 
                   Homo sapiens  clone RP11-7F18, WORKING DRAFT SEQUENCE, 19 unordered 
                 
                   Homo sapiens 
                 
                 35,642 
                 30-Nov-99 
               
               
                   
                   
                   
                   
                   
                 pieces. 
               
               
                 rxa01737 
                 1182 
                 GB_BA1: SCGD3 
                 33779 
                 AL096822 
                   Streptomyces coelicolor  cosmid GD3. 
                 
                   Streptomyces coelicolor 
                 
                 38,054 
                 8-Jul-99 
               
               
                   
                   
                 GB_HTG1: CNS01DSB 
                 222193 
                 AL121768 
                   Homo sapiens  chromosome 14 clone R-976B16, *** SEQUENCING IN PROGRESS 
                 
                   Homo sapiens 
                 
                 35,147 
                 05-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 ***, in ordered pieces. 
               
               
                   
                   
                 GB_HTG1: CNS01DSB 
                 222193 
                 AL121768 
                   Homo sapiens  chromosome 14 clone R-976B16, *** SEQUENCING IN PROGRESS 
                 
                   Homo sapiens 
                 
                 35,147 
                 05-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 ***, in ordered pieces. 
               
               
                 rxa01762 
                 1659 
                 GB_BA1: MTCI28 
                 36300 
                 Z97050 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 10/162. 
                 
                   Mycobacterium 
                 
                 49,574 
                 23-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: SC6G10 
                 36734 
                 AL049497 
                   Streptomyces coelicolor  cosmid 6G10. 
                 
                   Streptomyces coelicolor 
                 
                 44,049 
                 24-MAR-1999 
               
               
                   
                   
                 GB_BA1: SCE29 
                 26477 
                 AL035707 
                   Streptomyces coelicolor  cosmid E29. 
                 
                   Streptomyces coelicolor 
                 
                 40,246 
                 12-MAR-1999 
               
               
                 rxa01764 
                 1056 
                 GB_PL2: SPAC343 
                 42947 
                 AL109739 
                   S. pombe  chromosome I cosmid c343. 
                 
                   Schizosaccharomyces 
                 
                 37,084 
                 6-Sep-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   pombe 
                 
               
               
                   
                   
                 GB_PL2: SPAC343 
                 42947 
                 AL109739 
                   S. pombe  chromosome I cosmid c343. 
                 
                   Schizosaccharomyces 
                 
                 34,890 
                 6-Sep-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   pombe 
                 
               
               
                 rxa01801 
                 1140 
                 GB_EST38: AW066306 
                 334 
                 AW066306 
                 687009D03.y1 687 Early embryo from Delaware  Zea mays  cDNA, mRNA 
                 
                   Zea mays 
                 
                 46,108 
                 12-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                   
                   
                 GB_GSS13: AQ484750 
                 375 
                 AQ484750 
                 RPCI-11-248N4.TV RPCI-11  Homo sapiens  genomic clone RPCI-11-248N4, 
                 
                   Homo sapiens 
                 
                 32,000 
                 24-Apr-99 
               
               
                   
                   
                   
                   
                   
                 genomic survey sequence. 
               
               
                   
                   
                 GB_GSS13: AQ489971 
                 252 
                 AQ489971 
                 RPCI-11-247N23.TV RPCI-11  Homo sapiens  genomic clone RPCI-11-247N23, 
                 
                   Homo sapiens 
                 
                 36,111 
                 24-Apr-99 
               
               
                   
                   
                   
                   
                   
                 genomic survey sequence. 
               
               
                 rxa01823 
                 900 
                 GB_BA1: SCI51 
                 40745 
                 AL109848 
                   Streptomyces coelicolor  cosmid I51. 
                 
                   Streptomyces coelicolor 
                 
                 35,779 
                 16-Aug-99 
               
               
                   
                   
                   
                   
                   
                   
                 A3(2) 
               
               
                   
                   
                 GB_BA1: ECU82598 
                 136742 
                 U82598 
                   Escherichia coli  genomic sequence of minutes 9 to 12. 
                 
                   Escherichia coli 
                 
                 39,211 
                 15-Jan-97 
               
               
                   
                   
                 GB_BA1: BSUB0018 
                 209510 
                 Z99121 
                   Bacillus subtilis  complete genome (section 18 of 21): from 3399551 to 3609060. 
                 
                   Bacillus subtilis 
                 
                 36,999 
                 26-Nov-97 
               
               
                 rxa01853 
                 675 
                 GB_BA1: MTCY227 
                 35946 
                 Z77724 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 114/162. 
                 
                   Mycobacterium 
                 
                 37,612 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_HTG3: AC010189 
                 265962 
                 AC010189 
                   Homo sapiens  clone RPCI11-296K13, *** SEQUENCING IN PROGRESS ***, 80 
                 
                   Homo sapiens 
                 
                 39,006 
                 16-Sep-99 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC010189 
                 265962 
                 AC010189 
                   Homo sapiens  clone RPCI11-296K13, *** SEQUENCING IN PROGRESS ***, 80 
                 
                   Homo sapiens 
                 
                 39,006 
                 16-Sep-99 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa01881 
                 558 
                 GB_HTG4: AC011117 
                 148447 
                 AC011117 
                   Homo sapiens  chromosome 4 clone 173_C_09 map 4, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 39,130 
                 14-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 10 ordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC011117 
                 148447 
                 AC011117 
                   Homo sapiens  chromosome 4 clone 173_C_09 map 4, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 39,130 
                 14-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 10 ordered pieces. 
               
               
                   
                   
                 GB_BA1: MTCY2B12 
                 20431 
                 Z81011 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 61/162. 
                 
                   Mycobacterium 
                 
                 37,893 
                 18-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                 rxa01894 
                 978 
                 GB_BA1: MTCY274 
                 39991 
                 Z74024 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 126/162. 
                 
                   Mycobacterium 
                 
                 37,229 
                 19-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_IN1: CELF46H5 
                 38886 
                 U41543 
                   Caenorhabditis elegans  cosmid F46H5. 
                 
                   Caenorhabditis elegans 
                 
                 38,525 
                 29-Nov-96 
               
               
                   
                   
                 GB_HTG3: AC009204 
                 115633 
                 AC009204 
                   Drosophila melanogaster  chromosome 2 clone BACR03E19 (D1033) RPCI-98 
                 
                   Drosophila melanogaster 
                 
                 31,579 
                 18-Aug-99 
               
               
                   
                   
                   
                   
                   
                 03.E.19 map 36E-37C strain y; cn bw sp, *** SEQUENCING IN PROGRESS ***, 94 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa01897 
                 666 
                 GB_HTG1: CEY48B6 
                 293827 
                 AL021151 
                   Caenorhabditis elegans  chromosome II clone Y48B6, *** SEQUENCING IN 
                 
                   Caenorhabditis elegans 
                 
                 34,703 
                 1-Apr-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, in unordered pieces. 
               
               
                   
                   
                 GB_HTG1: CEY48B6 
                 293827 
                 AL021151 
                   Caenorhabditis elegans  chromosome II clone Y48B6, *** SEQUENCING IN 
                 
                   Caenorhabditis elegans 
                 
                 34,703 
                 1-Apr-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, in unordered pieces. 
               
               
                   
                   
                 GB_HTG1: CEY53F4_2 
                 110000 
                 Z92860 
                   Caenorhabditis elegans  chromosome II clone Y53F4, *** SEQUENCING IN 
                 
                   Caenorhabditis elegans 
                 
                 33,333 
                 15-Oct-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, in unordered pieces. 
               
               
                 rxa01946 
                 1298 
                 GB_BA1: MTV007 
                 32806 
                 AL021184 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 64/162. 
                 
                   Mycobacterium 
                 
                 65,560 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: SC5F2A 
                 40105 
                 AL049587 
                   Streptomyces coelicolor  cosmid 5F2A. 
                 
                   Streptomyces coelicolor 
                 
                 50,648 
                 24-MAY-1999 
               
               
                   
                   
                 GB_BA1: SCARD1GN 
                 2321 
                 X84374 
                   S. capreolus  ard1 gene. 
                 
                   Streptomyces capreolus 
                 
                 44,973 
                 23-Aug-95 
               
               
                 rxa01980 
                 756 
                 GB_PL2: AC008262 
                 99698 
                 AC008262 
                 Genomic sequence for  Arabidopsis thaliana  BAC F4N2 from chromosome I, 
                 
                   Arabidopsis thaliana 
                 
                 35,310 
                 21-Aug-99 
               
               
                   
                   
                   
                   
                   
                 complete sequence. 
               
               
                   
                   
                 GB_PL1: AB013388 
                 73428 
                 AB013388 
                   Arabidopsis thaliana  genomic DNA, chromosome 5, TAC clone: K19E1, complete 
                 
                   Arabidopsis thaliana 
                 
                 35,505 
                 20-Nov-99 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                   
                   
                 GB_PL1: AB013388 
                 73428 
                 AB013388 
                   Arabidopsis thaliana  genomic DNA, chromosome 5, TAC clone: K19E1, complete 
                 
                   Arabidopsis thaliana 
                 
                 39,973 
                 20-Nov-99 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                 rxa01983 
                 630 
                 GB_HTG4: AC006467 
                 175695 
                 AC006467 
                   Drosophila melanogaster  chromosome 2 clone BACR03L08 (D532) RPCI-98 03.L.8 
                 
                   Drosophila melanogaster 
                 
                 36,672 
                 27-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 map 40A-40C strain y; cn bw sp, *** SEQUENCING IN 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 9 unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC006467 
                 175695 
                 AC006467 
                   Drosophila melanogaster  chromosome 2 clone BACR03L08 (D532) RPCI-98 03.L.8 
                 
                   Drosophila melanogaster 
                 
                 36,672 
                 27-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 map 40A-40C strain y; cn bw sp, *** SEQUENCING IN 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 9 unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC006467 
                 175695 
                 AC006467 
                   Drosophila melanogaster  chromosome 2 clone BACR03L08 (D532) RPCI-98 03.L.8 
                 
                   Drosophila melanogaster 
                 
                 32,367 
                 27-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 map 40A-40C strain y; cn bw sp, *** SEQUENCING IN PROGRESSo ***, 9 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa02020 
                 1111 
                 GB_BA1: CGDNAAROP 
                 2612 
                 X85965 
                   C. glutamicum  ORF3 and aroP gene. 
                 
                   Corynebacterium 
                 
                 100,000 
                 30-Nov-97 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_PAT: A58887 
                 1612 
                 A58887 
                 Sequence 1 from Patent WO9701637. 
                 unidentified 
                 100,000 
                 06-MAR-1998 
               
               
                   
                   
                 GB_BA1: STYCARABA 
                 4378 
                 M95047 
                   Salmonella typhimurium  transport protein, complete cds, and transfer RNA-Arg. 
                 
                   Salmonella typhimurium 
                 
                 50,547 
                 13-MAR-1996 
               
               
                 rxa02029 
                 1437 
                 GB_HTG2: AC003023 
                 104768 
                 AC003023 
                   Homo sapiens  chromosome 11 clone pDJ363p2, *** SEQUENCING IN PROGRESS 
                 
                   Homo sapiens 
                 
                 35,820 
                 21-OCT-1997 
               
               
                   
                   
                   
                   
                   
                 ****, 22 unordered pieces. 
               
               
                   
                   
                 GB_HTG2: AC003023 
                 104768 
                 AC003023 
                   Homo sapiens  chromosome 11 clone pDJ363p2, *** SEQUENCING IN PROGRESS 
                 
                   Homo sapiens 
                 
                 35,820 
                 21-OCT-1997 
               
               
                   
                   
                   
                   
                   
                 ***, 22 unordered pieces. 
               
               
                   
                   
                 GB_HTG2: HS118B18 
                 104729 
                 AL034344 
                   Homo sapiens  chromosome 6 clone RP1-118B18 map p24.1-25.3, *** 
                 
                   Homo sapiens 
                 
                 34,355 
                 03-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 SEQUENCING IN PROGRESS ***, in unordered pieces. 
               
               
                 rxa02030 
                 1509 
                 GB_PR4: AC007695 
                 63247 
                 AC007695 
                   Homo sapiens  12q24 BAC RPCI11-124N23 (Roswell Park Cancer Institute Human 
                 
                   Homo sapiens 
                 
                 38,681 
                 1-Sep-99 
               
               
                   
                   
                   
                   
                   
                 BAC Library) complete sequence. 
               
               
                   
                   
                 GB_PR4: AC006464 
                 99908 
                 AC006464 
                   Homo sapiens  BAC clone NH0436C12 from 2, complete sequence. 
                 
                   Homo sapiens 
                 
                 35,445 
                 22-OCT-1999 
               
               
                   
                   
                 GB_PR4: AC006464 
                 99908 
                 AC006464 
                   Homo sapiens  BAC clone NH0436C12 from 2, complete sequence. 
                 
                   Homo sapiens 
                 
                 35,968 
                 22-OCT-1999 
               
               
                 rxa02073 
                 1653 
                 GB_BA1: CGGDHA 
                 2037 
                 X72855 
                   C. glutamicum  GDHA gene. 
                 
                   Corynebacterium 
                 
                 39,655 
                 24-MAY-1993 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: CGGDH 
                 2037 
                 X59404 
                   Corynebacterium glutamicum , gdh gen for glutamate dehydrogenase. 
                 
                   Corynebacterium 
                 
                 44,444 
                 30-Jul-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA2: SC2H4 
                 25970 
                 AL031514 
                   Streptomyces coelicolor  cosmid 2H4. 
                 
                   Streptomyces coelicolor 
                 
                 38,452 
                 19-OCT-1999 
               
               
                   
                   
                   
                   
                   
                   
                 A3(2) 
               
               
                 rxa02074 
               
               
                 rxa02095 
                 1527 
                 GB_EST18: AA703380 
                 471 
                 AA703380 
                 zj12b06.s1 Soares_fetal_liver_spleen_1NFLS_S1 Home sapiens cDNA clone 
                 
                   Home sapiens 
                 
                 36,518 
                 24-DEC-1997 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 450035 3′ similar to contains LTR5.t3 LTR5 repetitive element:, mRNA 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                   
                   
                 GB_HTG6: AC009769 
                 122911 
                 AC009769 
                   Homo sapiens  chromosome 8 clone RP11-202I12 map 8, LOW-PASS SEQUENCE 
                 
                   Homo sapiens 
                 
                 35,473 
                 07-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 SAMPLING. 
               
               
                   
                   
                 GB_EST7: W70175 
                 436 
                 W70175 
                 zd52c02.r1 Soares_fetal_heart_NbHH19W  Homo sapiens  cDNA clone 
                 
                   Homo sapiens 
                 
                 34,174 
                 16-OCT-1996 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 344258 5′ similar to contains LTR5.b2 LTR5 repetitive element;, mRNA 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                 rxa02099 
                 373 
                 GB_BA1: CAJ10319 
                 5368 
                 AJ010319 
                   Corynebacterium glutamicum  amtP, glnB, glnD genes and partial ftsY and srp genes. 
                 
                   Corynebacterium 
                 
                 100,000 
                 14-MAY-1999 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_HTG3: AC011509 
                 111353 
                 AC011509 
                   Homo sapiens  chromosome 19 clone CITB-H1_2189E23, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 33,423 
                 07-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 35 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC011509 
                 111353 
                 AC011509 
                   Homo sapiens  chromosome 19 clone CITB-H1_2189E23, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 33,423 
                 07-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 35 unordered pieces. 
               
               
                 rxa02115 
                 1197 
                 GB_HTG5: AC010126 
                 175986 
                 AC010126 
                   Homo sapiens  clone GS502B02, *** SEQUENCING IN PROGRESS ***, 
                 
                   Homo sapiens 
                 
                 36,717 
                 13-Nov-99 
               
               
                   
                   
                   
                   
                   
                 3 unordered pieces. 
               
               
                   
                   
                 GB_HTG5: AC010126 
                 175986 
                 AC010126 
                   Homo sapiens  clone GS502B02, *** SEQUENCING IN PROGRESS ***, 
                 
                   Homo sapiens 
                 
                 36,092 
                 13-Nov-99 
               
               
                   
                   
                   
                   
                   
                 3 unordered pieces. 
               
               
                   
                   
                 GB_PR1: HUMHM145 
                 2214 
                 D10925 
                 Human mRNA for HM145. 
                 
                   Homo sapiens 
                 
                 39,171 
                 3-Feb-99 
               
               
                 rxa02128 
                 1818 
                 GB_BA1: MTCY190 
                 34150 
                 Z70283 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 98/162. 
                 
                   Mycobacterium 
                 
                 38,682 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: MTCY190 
                 34150 
                 Z70283 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 98/162. 
                 
                   Mycobacterium 
                 
                 35,746 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_GSS10: AQ161109 
                 738 
                 AQ161109 
                 nbxb0006D03r CUGI Rice BAC Library  Oryza sativa  genomic clone nbxb0006D03r, 
                 
                   Oryza sativa 
                 
                 38,482 
                 12-Sep-98 
               
               
                   
                   
                   
                   
                   
                 genomic survey sequence. 
               
               
                 rxa02133 
                 329 
                 GB_BA2: MPAE000058 
                 28530 
                 AE000058 
                   Mycoplasma pneumoniae  section 58 of 63 of the complete genome. 
                 
                   Mycoplasma pneumoniae 
                 
                 32,317 
                 18-Nov-96 
               
               
                   
                   
                 GB_HTG4: AC008308 
                 151373 
                 AC008308 
                   Drosophila melanogaster  chromosome 3 clone BACR10M16 (D743) RPCI-98 
                 
                   Drosophila melanogaster 
                 
                 34,579 
                 20-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 10.M.16 map 93C-93D strain y; cn bw sp, 
               
               
                   
                   
                   
                   
                   
                 *** SEQUENCING IN PROGRESS ***, 186 unordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC008308 
                 151373 
                 AC008308 
                   Drosophila melanogaster  chromosome 3 clone BACR10M16 (D743) RPCI-98 
                 
                   Drosophila melanogaster 
                 
                 34,579 
                 20-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 10.M.16 map 93C-93D strain y; cn bw sp, 
               
               
                   
                   
                   
                   
                   
                 *** SEQUENCING IN PROGRESS ***, 186 unordered pieces. 
               
               
                 rxa02150 
                 924 
                 GB_EST37: AW012260 
                 358 
                 AW012260 
                 um06e09.y1 Sugano mouse kidney mkia  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 39,385 
                 10-Sep-99 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 2182312 5′ similar to SW: AMPL_BOVIN P00727 CYTOSOL 
               
               
                   
                   
                   
                   
                   
                 AMINOPEPTIDASE;, mRNA sequence. 
               
               
                   
                   
                 GB_GSS3: B87734 
                 389 
                 B87734 
                 RPCI11-30D24.TP RPCI-11  Homo sapiens  genomic clone RPCI-11-30D24, genomic 
                 
                   Homo sapiens 
                 
                 37,629 
                 9-Apr-99 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                   
                   
                 GB_PR4: AC005042 
                 192218 
                 AC005042 
                   Homo sapiens  clone NH0552E01, complete sequence. 
                 
                   Homo sapiens 
                 
                 36,901 
                 14-Jan-99 
               
               
                 rxa02171 
                 1776 
                 GB_BA2: AF010496 
                 189370 
                 AF010496 
                   Rhodobacter capsulatus  strain SB1003, partial genome. 
                 
                   Rhodobacter capsulatus 
                 
                 53,714 
                 12-MAY-1998 
               
               
                   
                   
                 GB_EST24: AI170522 
                 367 
                 AI170522 
                 EST216450 Normalized rat lung, Bento Soares  Rattus  sp. cDNA clone RLUCO75 3′ 
                   Rattus  sp. 
                 44,186 
                 20-Jan-99 
               
               
                   
                   
                   
                   
                   
                 end, mRNA sequence. 
               
               
                   
                   
                 GB_PL1: PHVDLECA 
                 1441 
                 K03288 
                   P. vulgaris  phytohemagglutinin gene encoding erythroagglutinating 
                 
                   Phaseolus vulgaris 
                 
                 39,103 
                 27-Apr-93 
               
               
                   
                   
                   
                   
                   
                 phytohemagglutinin (PHA-E), complete cds. 
               
               
                 rxa02173 
                 1575 
                 GB_BA1: CGGLTG 
                 3013 
                 X66112 
                   C. glutamicum  glt gene for citrate synthase and ORF. 
                 
                   Corynebacterium 
                 
                 44,118 
                 17-Feb-95 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: CGGLTG 
                 3013 
                 X66112 
                   C. glutamicum  glt gene for citrate synthase and ORF. 
                 
                   Corynebacterium 
                 
                 36,189 
                 17-Feb-95 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA2: AE000104 
                 10146 
                 AE000104 
                   Rhizobium  sp. NGR234 plasmid pNGR234a, section 41 of 46 of the complete 
                   Rhizobium  sp. NGR234 
                 38,487 
                 12-DEC-1997 
               
               
                   
                   
                   
                   
                   
                 plasmid sequence. 
               
               
                 rxa02224 
                 1920 
                 GB_BA2: CXU21300 
                 8990 
                 U21300 
                   Corynebacterium striatum  hypothetical protein YbhB gene, partial cds; ABC 
                 
                   Corynebacterium 
                 
                 37,264 
                 9-Apr-99 
               
               
                   
                   
                   
                   
                   
                 transporter TetB (tetB), ABC transporter TetA (tetA), transposase, 23S rRNA 
                 
                   striatum 
                 
               
               
                   
                   
                   
                   
                   
                 methyltransferase, and transposase genes, complete cds; and unknown 
               
               
                   
                   
                   
                   
                   
                 genes. 
               
               
                   
                   
                 GB_HTG3: AC009185 
                 87184 
                 AC009185 
                   Homo sapiens  chromosome 5 clone CIT-HSPC_248O19, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 36,459 
                 07-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 2 ordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC009185 
                 87184 
                 AC009185 
                   Homo sapiens  chromosome 5 clone CIT-HSPC_248O19, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 36,459 
                 07-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 2 ordered pieces. 
               
               
                 rxa02225 
                 905 
                 GB_BA2: MPAE000058 
                 28530 
                 AE000058 
                   Mycoplasma pneumoniae  section 58 of 63 of the complete genome. 
                 
                   Mycoplasma pneumoniae 
                 
                 35,498 
                 18-Nov-96 
               
               
                   
                   
                 GB_EST26: AI337275 
                 618 
                 AI337275 
                 tb96h11.x1 NCI_CGAP_Co16  Homo sapiens  cDNA clone IMAGE: 
                 
                   Homo sapiens 
                 
                 35,589 
                 18-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 2062245 3′ similar to TR: Q15392 Q15392 ORF, COMPLETE CDS.;, 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                   
                   
                 GB_EST26: AI337275 
                 618 
                 AI337275 
                 tb96h11.x1 NCI_CGAP_Co16  Homo sapiens  cDNA clone IMAGE: 
                 
                   Homo sapiens 
                 
                 42,786 
                 18-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 2062245 3′ similar to TR: Q15392 Q15392 ORF, COMPLETE CDS.;, 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                 rxa02233 
                 1410 
                 GB_BA1: ERWPNLB 
                 1291 
                 M65057 
                   Erwinia carotovora  pectin lyase (pnl) gene, complete cds. 
                 
                   Erwinia carotovora 
                 
                 37,780 
                 26-Apr-93 
               
               
                   
                   
                 GB_EST30: AV021947 
                 313 
                 AV021947 
                 AV021947  Mus musculus  18-day embryo C57BL/6J  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 39,423 
                 28-Aug-99 
               
               
                   
                   
                   
                   
                   
                 1190024M23, mRNA sequence. 
               
               
                   
                   
                 GB_EST33: AV087117 
                 251 
                 AV087117 
                 AV087117  Mus musculus  tongue C57BL/6J adult  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 47,410 
                 25-Jun-99 
               
               
                   
                   
                   
                   
                   
                 2310028C15, mRNA sequence. 
               
               
                 rxa02253 
                 1050 
                 GB_EST11: AA250210 
                 532 
                 AA250210 
                 mx79g10.r1 Soares mouse NML  Mus musculus  cDNA clone IMAGE: 692610 5′ 
                 
                   Mus musculus 
                 
                 36,136 
                 12-MAR-1997 
               
               
                   
                   
                   
                   
                   
                 similar to TR: E236517 E236517 F44G4.1;, mRNA sequence. 
               
               
                   
                   
                 GB_EST11: AA250210 
                 532 
                 AA250210 
                 mx79g10.r1 Soares mouse NML  Mus musculus  cDNA clone IMAGE: 692610 5′ 
                 
                   Mus musculus 
                 
                 36,202 
                 12-MAR-1997 
               
               
                   
                   
                   
                   
                   
                 similar to TR: E236517 E236517 F44G4.1;, mRNA sequence. 
               
               
                 rxa02261 
                 1479 
                 GB_BA1: CGL007732 
                 4460 
                 AJ007732 
                   Corynebacterium glutamicum  3′ ppc gene, secG gene, amt gene, ocd gene and 5′ 
                 
                   Corynebacterium 
                 
                 100,000 
                 7-Jan-99 
               
               
                   
                   
                   
                   
                   
                 soxA gene. 
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: CGAMTGENE 
                 2028 
                 X93513 
                   C. glutamicum  amt gene. 
                 
                   Corynebacterium 
                 
                 100,000 
                 29-MAY-1996 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: CORPEPC 
                 4885 
                 M25819 
                   C. glutamicum  phosphoenolpyruvate carboxylase gene, complete cds. 
                 
                   Corynebacterium 
                 
                 100,000 
                 15-DEC-1995 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                 rxa02268 
                 1023 
                 GB_PL2: AF087130 
                 3478 
                 AF087130 
                   Neurospora crassa  siderophore regulation protein (sre) gene, complete cds. 
                 
                   Neurospora crassa 
                 
                 39,268 
                 22-OCT-1998 
               
               
                   
                   
                 GB_EST30: AI663709 
                 408 
                 AI663709 
                 ud47a06.y1 Soares mouse mammary gland NbMMG  Mus musculus  cDNA clone 
                 
                   Mus musculus 
                 
                 41,523 
                 10-MAY-1999 
               
               
                   
                   
                   
                   
                   
                 IMAGE: 1449010 5′ similar to TR: O75585 O75585 MITOGEN- AND STRESS- 
               
               
                   
                   
                   
                   
                   
                 ACTIVATED PROTEIN KINASE-2;, mRNA sequence. 
               
               
                   
                   
                 GB_RO: AF074714 
                 3120 
                 AF074714 
                   Mus musculus  mitogen- and stress-activated protein kinase-2 (mMSK2) mRNA, 
                 
                   Mus musculus 
                 
                 38,347 
                 24-OCT-1998 
               
               
                   
                   
                   
                   
                   
                 complete cds. 
               
               
                 rxa02269 
                 1095 
                 GB_GSS4: AQ742825 
                 847 
                 AQ742825 
                 HS_5482_B2_A04_T7A RPCI-11 Human Male BAC Library  Homo sapiens   
                 
                   Homo sapiens 
                 
                 37,703 
                 16-Jul-99 
               
               
                   
                   
                   
                   
                   
                 genomic clone Plate = 1058 Col = 8 Row = B, genomic survey sequence. 
               
               
                   
                   
                 GB_HTG3: AC009293 
                 162944 
                 AC009293 
                   Homo sapiens  chromosome 18 clone 53_I_06 map 18, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 37,006 
                 13-Aug-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 15 unordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC009293 
                 162944 
                 AC009293 
                   Homo sapiens  chromosome 18 clone 53_I_06 map 18, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 37,006 
                 13-Aug-99 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 15 unordered pieces. 
               
               
                 rxa02309 
                 1173 
                 GB_BA1: MTY25D10 
                 40838 
                 Z95558 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 28/162. 
                 
                   Mycobacterium 
                 
                 52,344 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: MSGY224 
                 40051 
                 AD000004 
                   Mycobacterium tuberculosis  sequence from clone y224. 
                 
                   Mycobacterium 
                 
                 52,344 
                 03-DEC-1996 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_HTG2: AC007163 
                 186618 
                 AC007163 
                   Homo sapiens  clone NH0091M05, *** SEQUENCING IN PROGRESS ***, 1 
                 
                   Homo sapiens 
                 
                 37,263 
                 23-Apr-99 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa02310 
                 1386 
                 GB_BA1: MTY25D10 
                 40838 
                 Z95558 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 28/162. 
                 
                   Mycobacterium 
                 
                 36,861 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: MSGY224 
                 40051 
                 AD000004 
                   Mycobacterium tuberculosis  sequence from clone y224. 
                 
                   Mycobacterium 
                 
                 36,861 
                 03-DEC-1996 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_PR3: HS279N11 
                 169998 
                 Z98255 
                 Human DNA sequence from PAC 279N11 on chromosome Xq11.2-13.3. 
                 
                   Homo sapiens 
                 
                 34,516 
                 23-Nov-99 
               
               
                 rxa02321 
                 1752 
                 GB_BA1: AB018531 
                 4961 
                 AB018531 
                   Corynebacterium glutamicum  dtsR1 and dtsR2 genes, complete cds. 
                 
                   Corynebacterium 
                 
                 99,030 
                 19-OCT-1998 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_PAT: E17019 
                 4961 
                 E17019 
                   Brevibacterium lactofermentum  dtsR and dtsR2 genes. 
                 
                   Corynebacterium 
                 
                 98,973 
                 28-Jul-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: AB018530 
                 2855 
                 AB018530 
                   Corynebacterium glutamicum  dtsR gene, complete cds. 
                 
                   Corynebacterium 
                 
                 99,030 
                 19-OCT-1998 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                 rxa02335 
                 1896 
                 GB_BA1: CGU35023 
                 3195 
                 U35023 
                   Corynebacterium glutamicum  thiosulfate sulfurtransferase (thtR) gene, partial cds, 
                 
                   Corynebacterium 
                 
                 99,947 
                 16-Jan-97 
               
               
                   
                   
                   
                   
                   
                 acyl CoA carboxylase (accBC) gene, complete cds. 
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: U00012 
                 33312 
                 U00012 
                   Mycobacterium leprae  cosmid B1308. 
                 
                   Mycobacterium leprae 
                 
                 40,247 
                 30-Jan-96 
               
               
                   
                   
                 GB_BA1: MTCY71 
                 42729 
                 Z92771 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 141/162. 
                 
                   Mycobacterium 
                 
                 67,568 
                 10-Feb-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                 rxa02364 
                 750 
                 GB_BA1: AP000006 
                 319000 
                 AP000006 
                   Pyrococcus horikoshii  OT3 genomic DNA, 1166001-1485000 nt. position (6/7). 
                 
                   Pyrococcus horikoshii 
                 
                 36,130 
                 8-Feb-99 
               
               
                   
                   
                 GB_BA1: AP000006 
                 319000 
                 AP000006 
                   Pyrococcus horikoshii  OT3 genomic DNA, 1166001-1485000 nt. position (6/7). 
                 
                   Pyrococcus horikoshii 
                 
                 34,543 
                 8-Feb-99 
               
               
                 rxa02372 
                 2010 
                 GB_HTG3: AC011461 
                 100974 
                 AC011461 
                   Homo sapiens  chromosome 19 clone CIT-HSPC_429L19, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 36,138 
                 07-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 4 ordered pieces. 
               
               
                   
                   
                 GB_HTG3: AC011461 
                 100974 
                 AC011461 
                   Homo sapiens  chromosome 19 clone CIT-HSPC_429L19, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 36,138 
                 07-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 4 ordered pieces. 
               
               
                   
                   
                 GB_EST21: AA992021 
                 279 
                 AA992021 
                 ot36c01.s1 Soares_testis_NHT  Homo sapiens  cDNA clone IMAGE: 1618848 3′, 
                 
                   Homo sapiens 
                 
                 41,219 
                 3-Jun-98 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                 rxa02397 
                 1119 
                 GB_HTG4: AC009273 
                 76175 
                 AC009273 
                   Arabidopsis thaliana  chromosome 1 clone T1N6, *** SEQUENCING IN PROGRESS 
                 
                   Arabidopsis thaliana 
                 
                 38,566 
                 12-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 ***, 2 ordered pieces. 
               
               
                   
                   
                 GB_HTG4: AC009273 
                 76175 
                 AC009273 
                   Arabidopsis thaliana  chromosome 1 clone T1N6, *** SEQUENCING IN PROGRESS 
                 
                   Arabidopsis thaliana 
                 
                 38,566 
                 12-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 ***, 2 ordered pieces. 
               
               
                   
                   
                 GB_BA1: D90826 
                 19493 
                 D90826 
                   E. coli  genomic DNA, Kohara clone #335(40.9-41.3 min.). 
                 
                   Escherichia coli 
                 
                 39,600 
                 21-MAR-1997 
               
               
                 rxa02424 
                 723 
                 GB_EST13: AA334108 
                 275 
                 AA334108 
                 EST38262 Embryo, 9 week  Homo sapiens  cDNA 5′ end, mRNA sequence. 
                 
                   Homo sapiens 
                 
                 38,603 
                 21-Apr-97 
               
               
                   
                   
                 GB_PR3: AC005224 
                 166687 
                 AC005224 
                   Homo sapiens  chromosome 17, clone hRPK.214_O_1, complete sequence. 
                 
                   Homo sapiens 
                 
                 36,111 
                 14-Aug-98 
               
               
                   
                   
                 GB_PR3: AC005224 
                 166687 
                 AC005224 
                   Homo sapiens  chromosome 17, clone hRPK.214_O_1, complete sequence. 
                 
                   Homo sapiens 
                 
                 33,427 
                 14-Aug-98 
               
               
                 rxa02426 
                 1656 
                 GB_PAT: A06664 
                 1350 
                 A06664 
                   B. stearothermophilus  lct gene. 
                 
                   Bacillus 
                 
                 39,936 
                 29-Jul-93 
               
               
                   
                   
                   
                   
                   
                   
                 
                   stearothermophilus 
                 
               
               
                   
                   
                 GB_PAT: A04115 
                 1361 
                 A04115 
                   B. stearothermophilus  recombinant lct gene. 
                 
                   synthetic construct 
                 
                 40,042 
                 17-Feb-97 
               
               
                   
                   
                 GB_BA1: BACLDHL 
                 1361 
                 M14788 
                   B. stearothermophilus  lct gene encoding L-lactate dehydrogenase, complete cds. 
                 
                   Bacillus 
                 
                 40,338 
                 26-Apr-93 
               
               
                   
                   
                   
                   
                   
                   
                 
                   stearothermophilus 
                 
               
               
                 rxa02487 
                 1827 
                 GB_BA2: AF007101 
                 32870 
                 AF007101 
                   Streptomyces hygroscopicus  putative pteridine-dependent dioxygenase, PKS 
                 
                   Streptomyces 
                 
                 43,298 
                 13-Jan-98 
               
               
                   
                   
                   
                   
                   
                 modules 1, 2, 3 and 4, and putative regulatory protein genes, complete cds and 
                 
                   hygroscopicus 
                 
               
               
                   
                   
                   
                   
                   
                 putative hydroxylase gene, partial cds. 
               
               
                   
                   
                 GB_BA1: MTCI364 
                 29540 
                 Z93777 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 52/162. 
                 
                   Mycobacterium 
                 
                 44,352 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA2: AF119621 
                 15986 
                 AF119621 
                   Pseudomonas abietaniphila  BKME-9 Ditl (ditl), dioxygenase DitA oxygenase 
                 
                   Pseudomonas 
                 
                 43,611 
                 28-Apr-99 
               
               
                   
                   
                   
                   
                   
                 component small subunit (ditA2), dioxygenase DitA oxygenase component large 
                 
                   abietaniphila 
                 
               
               
                   
                   
                   
                   
                   
                 subunit (ditA1), DitH (ditH), DitG (ditG), DitF (ditF), DitR (ditR), DitE (ditE), DitD 
               
               
                   
                   
                   
                   
                   
                 (ditD), aromatic diterpenoid extradiol ring-cleavage dioygenase (ditC), DitB (ditB), 
               
               
                   
                   
                   
                   
                   
                 and dioxygenase DitA ferredoxin component (ditA3) genes, complete cds; and 
               
               
                   
                   
                   
                   
                   
                 unknown genes. 
               
               
                 rxa02511 
                 780 
                 GB_PR4: AC002470 
                 235395 
                 AC002470 
                   Homo sapiens  Chromosome 22q11.2 BAC Clone b135h6 in BCRL2-GGT Region, 
                 
                   Homo sapiens 
                 
                 37,971 
                 30-Nov-99 
               
               
                   
                   
                   
                   
                   
                 complete sequence. 
               
               
                   
                   
                 GB_PR4: AC002472 
                 147100 
                 AC002472 
                   Homo sapiens  Chromosome 22q11.2 PAC Clone p_n5 in BCRL2-GGT Region, 
                 
                   Homo sapiens 
                 
                 38,239 
                 13-Sep-99 
               
               
                   
                   
                   
                   
                   
                 complete sequence. 
               
               
                   
                   
                 GB_EST34: AI806938 
                 118 
                 AI806938 
                 wf24b07.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE: 
                 
                   Homo sapiens 
                 
                 38,983 
                 7-Jul-99 
               
               
                   
                   
                   
                   
                   
                 2356501 3′ similar to SW: PLZF_HUMAN Q05516 ZINC FINGER PROTEIN 
               
               
                   
                   
                   
                   
                   
                 PLZF;, mRNA sequence. 
               
               
                 rxa02512 
                 1086 
                 GB_BA1: MTCY1A10 
                 25949 
                 Z95387 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 117/162. 
                 
                   Mycobacterium 
                 
                 37,407 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: MLCL581 
                 36225 
                 Z96801 
                   Mycobacterium leprae  cosmid L581. 
                 
                   Mycobacterium leprae 
                 
                 43,193 
                 24-Jun-97 
               
               
                   
                   
                 GB_OV: GGU43396 
                 2738 
                 U43396 
                   Gallus gallus  tropomyosin receptor kinase A (ctrkA) mRNA, complete cds. 
                 
                   Gallus gallus 
                 
                 38,789 
                 18-Jan-96 
               
               
                 rxa02527 
                 1452 
                 GB_BA2: AF008220 
                 220060 
                 AF008220 
                   Bacillus subtilis  rrnB-dnaB genomic region. 
                 
                   Bacillus subtilis 
                 
                 37,395 
                 4-Feb-98 
               
               
                   
                   
                 GB_BA2: AF008220 
                 220060 
                 AF008220 
                   Bacillus subtilis  rrnB-dnaB genomic region. 
                 
                   Bacillus subtilis 
                 
                 36,218 
                 4-Feb-98 
               
               
                   
                   
                 GB_HTG2: AC005861 
                 112369 
                 AC005861 
                   Arabidopsis thaliana  clone F23B24, *** SEQUENCING IN PROGRESS ***, 6 
                 
                   Arabidopsis thaliana 
                 
                 38,407 
                 29-Apr-99 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa02547 
                 2262 
                 GB_PL1: AB006530 
                 7344 
                 AB006530 
                   Citrullus lanatus  Sat gene for serine acetyltransferase, complete cds and 5′-flanking 
                 
                   Citrullus lanatus 
                 
                 35,449 
                 20-Aug-97 
               
               
                   
                   
                   
                   
                   
                 region. 
               
               
                   
                   
                 GB_PL1: CNASA 
                 5729 
                 D85624 
                   Citrullus vulgaris  serine acetyltransferase (Sat) DNA, complete cds. 
                 
                   Citrullus lanatus 
                 
                 35,449 
                 6-Feb-99 
               
               
                   
                   
                 GB_PL1: AB006530 
                 7344 
                 AB006530 
                   Citrullus lanatus  Sat gene for serine acetyltransferase, complete cds and 5′-flanking 
                 
                   Citrullus lanatus 
                 
                 34,646 
                 20-Aug-97 
               
               
                   
                   
                   
                   
                   
                 region. 
               
               
                 rxa02566 
                 1332 
                 GB_EST32: AI727189 
                 619 
                 AI727189 
                 BNLGHI7498 Six-day Cotton fiber  Gossypium hirsutum  cDNA 5′ similar to 
                 
                   Gossypium hirsutum 
                 
                 35,099 
                 11-Jun-99 
               
               
                   
                   
                   
                   
                   
                 (AB020715) KIAA0908 protein [ Homo sapiens ], mRNA sequence. 
               
               
                   
                   
                 GB_BA1: CGPUTP 
                 3791 
                 Y09163 
                   C. glutamicum  putP gene. 
                 
                   Corynebacterium 
                 
                 38,562 
                 8-Sep-97 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_PL2: SPAC13G6 
                 33481 
                 Z54308 
                   S. pombe  chromosome I cosmid c13G6. 
                 
                   Schizosaccharomyces 
                 
                 35,774 
                 18-OCT-1999 
               
               
                   
                   
                   
                   
                   
                   
                 
                   pombe 
                 
               
               
                 rxa02571 
                 1152 
                 GB_BA1: CGU43535 
                 2531 
                 U43535 
                   Corynebacterium glutamicum  multidrug resistance protein (cmr) gene, complete cds. 
                 
                   Corynebacterium 
                 
                 41,872 
                 9-Apr-97 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_EST35: AI857385 
                 488 
                 AI857385 
                 wl55e03.x1 NCI_CGAP_Brn25  Homo sapiens  cDNA clone IMAGE: 2428828 3′, 
                 
                   Homo sapiens 
                 
                 39,139 
                 26-Aug-99 
               
               
                   
                   
                   
                   
                   
                 mRNA sequence. 
               
               
                   
                   
                 GB_BA1: CGU43535 
                 2531 
                 U43535 
                   Corynebacterium glutamicum  multidrug resistance protein (cmr) gene, complete cds. 
                 
                   Corynebacterium 
                 
                 38,552 
                 9-Apr-97 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                 rxa02578 
                 1227 
                 GB_PL1: AB016871 
                 79109 
                 AB016871 
                   Arabldopsis thaliana  genomic DNA, chromosome 5, TAC clone: K16L22, complete 
                 
                   Arabidopsis thaliana 
                 
                 34,213 
                 20-Nov-99 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                   
                   
                 GB_PL1: AB025602 
                 55790 
                 AB025602 
                   Arabidopsis thaliana  genomic DNA, chromosome 5, BAC clone: F14A1, complete 
                 
                   Arabidopsis thaliana 
                 
                 36,461 
                 20-Nov-99 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                   
                   
                 GB_IN1: CELF36H9 
                 35985 
                 AF016668 
                   Caenorhabditis elegans  cosmid F36H9. 
                 
                   Caenorhabditis elegans 
                 
                 35,977 
                 8-Aug-97 
               
               
                 rxa02581 
                 1983 
                 GB_BA1: MTV005 
                 37840 
                 AL010186 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 51/162. 
                 
                   Mycobacterium 
                 
                 38,517 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: MTV005 
                 37840 
                 AL010186 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 51/162. 
                 
                   Mycobacterium 
                 
                 39,173 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                 rxa02582 
                 4953 
                 GB_BA1: MTV026 
                 23740 
                 AL022076 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 157/162. 
                 
                   Mycobacterium 
                 
                 38,548 
                 24-Jun-99 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: MTCY338 
                 29372 
                 Z74697 
                   Mycobacterium tuberculosis  H37Rv complete genome; segment 127/162. 
                 
                   Mycobacterium 
                 
                 46,263 
                 17-Jun-98 
               
               
                   
                   
                   
                   
                   
                   
                 
                   tuberculosis 
                 
               
               
                   
                   
                 GB_BA1: SEERYABS 
                 20444 
                 X62569 
                   S. erythraea  eryA gene for 6-deoxyerythronolyde B synthase II &amp; III. 
                 
                   Saccharopolyspora 
                 
                 45,053 
                 28-Feb-92 
               
               
                   
                   
                   
                   
                   
                   
                 
                   erythraea 
                 
               
               
                 rxa02583 
                 1671 
                 GB_BA2: AF113605 
                 1593 
                 AF113605 
                   Streptomyces coelicolor  proplonyl-CoA carboxylase complex B subunit (pccB) gene, 
                 
                   Streptomyces coelicolor 
                 
                 58,397 
                 08-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 complete cds. 
               
               
                   
                   
                 GB_BA1: SC1C2 
                 42210 
                 AL031124 
                   Streptomyces coelicolor  cosmid 1C2. 
                 
                   Streptomyces coelicolor 
                 
                 52.916 
                 15-Jan-99 
               
               
                   
                   
                 GB_BA1: AB018531 
                 4961 
                 AB018531 
                   Corynebacterium glutamicum  dtsR1 and dtsR2 genes, complete cds. 
                 
                   Corynebacterium 
                 
                 58,809 
                 19-OCT-1998 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                 rxa02599 
                 600 
                 GB_BA1: AEMML 
                 2585 
                 X99639 
                   Ralstonia eutropha  mmlH, mmlI &amp; mmlJ genes. 
                 
                   Ralstonia eutropha 
                 
                 35,264 
                 22-Jan-98 
               
               
                   
                   
                 GB_EST15: AA508926 
                 422 
                 AA508926 
                 MBAFCW1C08T3  Brugia malayi  adult female cDNA (SAW96MLW-BmAF)  Brugia   
                 
                   Brugia malayi 
                 
                 43,377 
                 8-Jul-97 
               
               
                   
                   
                   
                   
                   
                   malayi  cDNA clone AFCW1C08 5′, mRNA sequence. 
               
               
                   
                   
                 GB_BA1: AEMML 
                 2585 
                 X99639 
                   Ralstonia eutropha  mmlH, mmlI &amp; mmlJ genes. 
                 
                   Ralstonia eutropha 
                 
                 41,148 
                 22-Jan-98 
               
               
                 rxa02634 
                 1734 
                 GB_BA1: SYNPOO 
                 1964 
                 X17439 
                   Synechocystis  ndhC, psbG genes for NDH-C, PSII-G and ORF157. 
                   Synechocystis  PCC6803 
                 38,145 
                 10-Feb-99 
               
               
                   
                   
                 GB_GSS9: AQ101527 
                 184 
                 AQ101527 
                 HS_2265_A1_E11_MF CIT Approved Human Genomic Sperm Library D  Homo   
                 
                   Homo sapiens 
                 
                 38,798 
                 27-Aug-98 
               
               
                   
                   
                   
                   
                   
                   sapiens  genomic clone Plate = 2265 Col = 21 Row = l, genomic survey sequence. 
               
               
                   
                   
                 GB_IN1: MNE133341 
                 399 
                 AJ133341 
                   Melarhaphe neritoides  partial caM gene, exons 1-2. 
                 
                   Melarhaphe neritoides 
                 
                 39,098 
                 2-Jun-99 
               
               
                 rxa02638 
                 999 
                 GB_BA2 AE001756 
                 10938 
                 AE001756 
                   Thermotoga maritima  section 68 of 136 of the complete genome. 
                 
                   Thermotoga maritima 
                 
                 40,104 
                 2-Jun-99 
               
               
                   
                   
                 GB_GSS12: AQ423878 
                 689 
                 AQ423878 
                 CITBI-E1-2575E20.TF CITBI-E1  Homo sapiens  genomic clone 2575E20, genomic 
                 
                   Homo sapiens 
                 
                 36,451 
                 23-MAR-1999 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                   
                   
                 GB_HTG2: AC006765 
                 274498 
                 AC006765 
                   Caenorhabditis elegans  clone Y43H11, *** SEQUENCING IN PROGRESS***, 7 
                 
                   Caenorhabditis elegans 
                 
                 39,072 
                 23-Feb-99 
               
               
                   
                   
                   
                   
                   
                 unordered pieces. 
               
               
                 rxa02659 
                 335 
                 GB_EST36: AI900317 
                 436 
                 AI900317 
                 sc04a02.y1 Gm-c1012  Glycine max  cDNA clone GENOME SYSTEMS CLONE 
                 
                   Glycine max 
                 
                 41,566 
                 06-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 ID: Gm-c1012-1155 5′ similar to SW: PRS6_SOLTU P54778 26S PROTEASE 
               
               
                   
                   
                   
                   
                   
                 REGULATORY SUBUNIT 6B HOMOLOG.;, mRNA sequence. 
               
               
                   
                   
                 GB_GSS12: AQ342831 
                 683 
                 AQ342831 
                 RPCI11-122K17.TJ RPCI-11  Homo sapiens  genomic clone RPCI-11-122K17, 
                 
                   Homo sapiens 
                 
                 34,762 
                 07-MAY-1999 
               
               
                   
                   
                   
                   
                   
                 genomic survey sequence. 
               
               
                   
                   
                 GB_EST36: AI900856 
                 779 
                 AI900856 
                 sb95c11.y1 Gm-c1012  Glycine max  cDNA clone GENOME SYSTEMS CLONE ID: 
                 
                   Glycine max 
                 
                 39,063 
                 06-DEC-1999 
               
               
                   
                   
                   
                   
                   
                 Gm-c1012-429 5′ similar to SW: PRS6_SOLTU P54778 26S PROTEASE 
               
               
                   
                   
                   
                   
                   
                 REGULATORY SUBUNIT 6B HOMOLOG.;, mRNA sequence. 
               
               
                 rxa02676 
                 1512 
                 GB_IN2: CELB0213 
                 39134 
                 AF039050 
                   Caenorhabditis elegans  cosmid B0213. 
                 
                   Caenorhabditis elegans 
                 
                 35,814 
                 2-Jun-99 
               
               
                   
                   
                 GB_GSS1: CNS00PZB 
                 364 
                 AL085157 
                   Arabidopsis thaliana  genome survey sequence SP6 end of BAC F10D11 of IGF 
                 
                   Arabidopsis thaliana 
                 
                 38,462 
                 28-Jun-99 
               
               
                   
                   
                   
                   
                   
                 library from strain Columbia of  Arabidopsis thaliana , genomic survey sequence. 
               
               
                   
                   
                 GB_RO: RNITPR2R 
                 10708 
                 X61677 
                 Rat ITPR2 gene for type 2 inositol triphosphate receptor. 
                 
                   Rattus norvegicus 
                 
                 37,543 
                 21-OCT-1991 
               
               
                 rxa02677 
                 882 
                 GB_RO: D89728 
                 5002 
                 D89728 
                   Mus musculus  mRNA for LOK, complete cds. 
                 
                   Mus musculus 
                 
                 38,829 
                 7-Feb-99 
               
               
                   
                   
                 GB_GSS8: AQ062004 
                 362 
                 AQ062004 
                 CIT-HSP-2346O14, TR CIT-HSP  Homo sapiens  genomic clone 2346O14, genomic 
                 
                   Homo sapiens 
                 
                 36,565 
                 31-Jul-98 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                   
                   
                 GB_GSS14: AQ555818 
                 462 
                 AQ555818 
                 HS_5230_B1_G06_SP6E RPCI-11 Human Male BAC Library Homo sapiens 
                 
                   Homo sapiens 
                 
                 36,534 
                 29-MAY-1999 
               
               
                   
                   
                   
                   
                   
                 genomic clone Plate = 806 Col = 11 Row = N, genomic survey sequence. 
               
               
                 rxa02691 
                 930 
                 GB_IN1: DME9736 
                 7411 
                 AJ009736 
                   Drosophila melanogaster  idefix retroelement: gag, pol and env genes, partial. 
                 
                   Drosophila melanogaster 
                 
                 36,522 
                 19-Jan-99 
               
               
                   
                   
                 GB_PR4: AC004801 
                 193561 
                 AC004801 
                   Homo sapiens  12q13.1 PAC RPCI1-228P16 (Roswell Park Cancer Institute Human 
                 
                   Homo sapiens 
                 
                 39,341 
                 2-Feb-99 
               
               
                   
                   
                   
                   
                   
                 PAC Library) complete sequence. 
               
               
                   
                   
                 GB_PR4: AC004801 
                 193561 
                 AC004801 
                   Homo sapiens  12q13.1 PAC RPCI1-228P16 (Roswell Park Cancer Institute Human 
                 
                   Homo sapiens 
                 
                 37,037 
                 2-Feb-99 
               
               
                   
                   
                   
                   
                   
                 PAC Library) complete sequence. 
               
               
                 rxa02718 
                 1170 
                 GB_EST34: AV132028 
                 258 
                 AV132028 
                 AV132028  Mus musculus  C57BL/6J 11-day embryo Mus musculus cDNA clone 
                 
                   Mus musculus 
                 
                 43,529 
                 1-Jul-99 
               
               
                   
                   
                   
                   
                   
                 2700087F01, mRNA sequence. 
               
               
                   
                   
                 GB_GSS10: AQ240654 
                 452 
                 AQ240654 
                 CIT-HSP-2385D24.TFB.1 CIT-HSP  Homo sapiens  genomic clone 2385D24, genomic 
                 
                   Homo sapiens 
                 
                 40,044 
                 30-Sep-98 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                   
                   
                 GB_GSS11: AQ309500 
                 576 
                 AQ309500 
                 CIT-HSP-2384D24.TFD CIT-HSP  Homo sapiens  genomic clone 2384D24, genomic 
                 
                   Homo sapiens 
                 
                 38,869 
                 22-DEC-1998 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                 rxa02749 
                 999 
                 GB_BA2: AF086791 
                 37867 
                 AF086791 
                   Zymomonas mobilis  strain ZM4 clone 67E10 carbamoylphosphate synthetase small 
                 
                   Zymomonas mobilis 
                 
                 39,024 
                 4-Nov-98 
               
               
                   
                   
                   
                   
                   
                 subunit (carA), carbamoylphosphate synthetase large subunit (carB), transcription 
               
               
                   
                   
                   
                   
                   
                 elongation factor (greA), enolase (eno), pyruvate dehydrogenase alpha 
               
               
                   
                   
                   
                   
                   
                 subunit (pdhA), pyruvate dehydrogenase beta subunit (pdhB), ribonuclease H (mh), 
               
               
                   
                   
                   
                   
                   
                 homoserine kinase homolog, alcohol dehydrogenase II (adhB), and 
               
               
                   
                   
                   
                   
                   
                 excinuclease ABC subunit A (uvrA) genes, complete cds; and unknown genes. 
               
               
                   
                   
                 GB_BA1: SYCSLRB 
                 146271 
                 D64000 
                   Synechocystis  sp. PCC6803 complete genome, 19/27, 2392729-2538999. 
                   Synechocystis  sp. 
                 34,573 
                 13-Feb-99 
               
               
                   
                   
                 GB_BA2: AE001306 
                 13316 
                 AE001306 
                   Chlamydia trachomatis  section 33 of 87 of the complete genome. 
                 
                   Chlamydia trachomatis 
                 
                 38,940 
                 2-Sep-98 
               
               
                 rxa02767 
                 906 
                 GB_BA2: AF126953 
                 1638 
                 AF126953 
                   Corynebacterium glutamicum  cystathionine gamma-synthase (metB) gene, complete 
                 
                   Corynebacterium 
                 
                 100,000 
                 10-Sep-99 
               
               
                   
                   
                   
                   
                   
                 cds. 
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: SCI5 
                 6661 
                 AL079332 
                   Streptomyces coelicolor  cosmid I5. 
                 
                   Streptomyces coelicolor 
                 
                 37,486 
                 16-Jun-99 
               
               
                   
                   
                 GB_PR3: HS90L6 
                 190837 
                 Z97353 
                 Human DNA sequence from clone 90L6 on chromosome 22q11.21-11.23. Contains 
                 
                   Homo sapiens 
                 
                 34,149 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 an RPL15 (60S Ribosomal Protein L15) pseudogene, ESTs, STSs and GSSs, 
               
               
                   
                   
                   
                   
                   
                 complete sequence. 
               
               
                 rxa02792 
                 876 
                 GB_BA2: AF099015 
                 5000 
                 AF099015 
                   Streptomyces coelicolor  strain A3(2) integrase (int), Fe-containing superoxide 
                 
                   Streptomyces coelicolor 
                 
                 36,721 
                 1-Jun-99 
               
               
                   
                   
                   
                   
                   
                 dismutase II (sodF2), Fe uptake system permease (ftrE), and Fe uptake system 
               
               
                   
                   
                   
                   
                   
                 integral membrane protein (ftrD) genes, complete cds. 
               
               
                   
                   
                 GB_BA1: ECOUW93 
                 338534 
                 U14003 
                   Escherichia coli  K-12 chromosomal region from 92.8 to 00.1 minutes. 
                 
                   Escherichia coli 
                 
                 38,787 
                 17-Apr-96 
               
               
                   
                   
                 GB_HTG3: AC011361 
                 186148 
                 AC011361 
                   Homo sapiens  chromosome 5 clone CIT-HSPC_482N19, *** SEQUENCING IN 
                 
                   Homo sapiens 
                 
                 43,577 
                 06-OCT-1999 
               
               
                   
                   
                   
                   
                   
                 PROGRESS ***, 69 unordered pieces. 
               
               
                 rxa02794 
                 1197 
                 GB_PR4: AC005998 
                 96556 
                 AC005998 
                   Homo sapiens  clone DJ0622E21, complete sequence. 
                 
                   Homo sapiens 
                 
                 37,298 
                 29-Jul-99 
               
               
                   
                   
                 GB_PR4: AC006008 
                 57554 
                 AC006008 
                   Homo sapiens  clone DJ0820A21, complete sequence. 
                 
                   Homo sapiens 
                 
                 36,638 
                 17-Jun-99 
               
               
                   
                   
                 GB_PR3: HSDJ73H14 
                 95556 
                 AL080272 
                 Human DNA sequence from clone 73H14 on chromosome Xq26.3-28, complete 
                 
                   Homo sapiens 
                 
                 39,726 
                 23-Nov-99 
               
               
                   
                   
                   
                   
                   
                 sequence. 
               
               
                 rxa02809 
                 375 
                 GB_RO: MUSSPCTLT 
                 3172 
                 M22527 
                 Mouse cytotoxic T lymphocyte-specific serine protease CCPII gene, complete cds. 
                 
                   Mus musculus 
                 
                 47,518 
                 19-Jan-96 
               
               
                   
                   
                 GB_RO: MUSGRC 
                 894 
                 M18459 
                 Mouse granzyme C serine esterase mRNA, complete cds. 
                 
                   Mus musculus 
                 
                 44,939 
                 12-Jun-93 
               
               
                   
                   
                 GB_RO: RNU57062 
                 880 
                 U57062 
                   Rattus norvegicus  natural killer cell protease 4 (RNKP-4) mRNA, complete cds. 
                 
                   Rattus norvegicus 
                 
                 41,554 
                 31-Jul-96 
               
               
                 rxa02811 
                 484 
                 GB_GSS6: AQ832862 
                 476 
                 AQ832862 
                 HS_5261_A2_E10_SP6E RPCI-11 Human Male BAC Library Homo sapiens 
                 
                   Homo sapiens 
                 
                 35,610 
                 27-Aug-99 
               
               
                   
                   
                   
                   
                   
                 genomic clone Plate = 837 Col = 20 Row = I, genomic survey sequence. 
               
               
                   
                   
                 GB_GSS5: AQ784593 
                 515 
                 AQ784593 
                 HS_3248_A2_F02_T7C CIT Approved Human Genomic Sperm Library D Homo 
                 
                   Homo sapiens 
                 
                 38,956 
                 3-Aug-99 
               
               
                   
                   
                   
                   
                   
                 sapiens genomic clone Plate = 3248 Col = 4 Row = K, genomic survey sequence. 
               
               
                   
                   
                 GB_GSS13: AQ473140 
                 397 
                 AQ473140 
                 CITBI-E1-2589G6.TF CITBI-E1  Homo sapiens  genomic clone 2589G6, genomic 
                 
                   Homo sapiens 
                 
                 34,761 
                 23-Apr-99 
               
               
                   
                   
                   
                   
                   
                 survey sequence. 
               
               
                 rxa02836 
                 678 
                 GB_EST18: AA696785 
                 316 
                 AA696785 
                 GM08392.5prime GM  Drosophila melanogaster  ovary BlueScript  Drosophila   
                 
                   Drosophila melanogaster 
                 
                 40,604 
                 28-Nov-98 
               
               
                   
                   
                   
                   
                   
                   melanogaster  cDNA clone GM08392 5prime, mRNA sequence. 
               
               
                   
                   
                 GB_EST18: AA696785 
                 316 
                 AA696785 
                 GM08392.5prime GM  Drosophila melanogaster  ovary BlueScript Drosophila 
                 
                   Drosophila melanogaster 
                 
                 38,281 
                 28-Nov-98 
               
               
                   
                   
                   
                   
                   
                 melanogaster cDNA clone GM08392 5prime, mRNA sequence. 
               
               
                 rxs03212 
                 1452 
                 GB_BA1: CGBETPGEN 
                 2339 
                 X93514 
                   C. glutamicum  betP gene. 
                 
                   Corynebacterium 
                 
                 99,931 
                 8-Sep-97 
               
               
                   
                   
                   
                   
                   
                   
                 
                   glutamicum 
                 
               
               
                   
                   
                 GB_BA1: SC5F2A 
                 40105 
                 AL049587 
                   Streptomyces coelicolor  cosmid 5F2A. 
                 
                   Streptomyces coelicolor 
                 
                 57,557 
                 24-MAY-1999 
               
               
                   
                   
                   
                   
                   
                   
                 A3(2) 
               
               
                   
                   
                 GB_BA2: AF008220 
                 220060 
                 AF008220 
                   Bacillus subtilis  rrnB-dnaB genomic region. 
                 
                   Bacillus subtilis 
                 
                 40,000 
                 4-Feb-98 
               
               
                 rxs03220 
                 725 
                 GB_PL1: CKHUP2 
                 2353 
                 X66855 
                   C. kessleri  HUP2 mRNA. 
                 
                   Chlorella kessleri 
                 
                 45,328 
                 17-Feb-97 
               
               
                   
                   
                 GB_EST38: AW048153 
                 383 
                 AW048153 
                 UI-M-BH1-alq-h-05-0-UI.s1 NIH_BMAP_M_S2  Mus musculus  cDNA 
                 
                   Mus musculus 
                 
                 41,758 
                 18-Sep-99 
               
               
                   
                   
                   
                   
                   
                 clone UI-M-BH1-alq-h-05-0-UI 3′, mRNA sequence. 
               
               
                   
                   
                 GB_PL1: CKHUP2 
                 2353 
                 X66855 
                   C. kessleri  HUP2 mRNA. 
                 
                   Chlorella kessleri 
                 
                 38,106 
                 17-Feb-97