Patent Publication Number: US-5633427-A

Title: Inbred corn line PHHB

Description:
REFERENCE TO RELATED APPLICATION 
     This is a continuation of prior application Ser. No. 08/189,004, filed Jan. 24, 1994, now U.S. Pat. No. 5,444,178. 
    
    
     FIELD OF THE INVENTION 
     This invention is in the field of corn breeding, specifically relating to an inbred corn line designated PHHB4. 
     BACKGROUND OF THE INVENTION 
     Plant Breeding 
     Field crops are bred through techniques that take advantage of the plant&#39;s method of pollination. A plant is self-pollinated if pollen from one flower is transferred to the same or another flower of the same plant. A plant is cross-pollinated if the pollen comes from a flower on a different plant. 
     Corn plants (Zea mays L.) can be bred by both self-pollination and cross-pollination techniques. Corn has separate male and female flowers on the same plant, located on the tassel and the ear, respectively. Natural pollination occurs in corn when wind blows pollen from the tassels to the silks that protrude from the tops of the incipient ears. 
     The development of a hybrid corn variety involves three steps: (1) the selection of plants from various germplasm pools; (2) the selfing of the selected plants for several generations to produce a series of inbred lines, which, although different from each other, breed true and are highly uniform; and (3) crossing the selected inbred lines with unrelated inbred lines to produce the hybrid progeny (F 1 ). During the inbreeding process in corn, the vigor of the lines decreases. Vigor is restored when two unrelated inbred lines are crossed to produce the hybrid progeny. An important consequence of the homozygosity and homogeneity of the inbred lines is that the hybrid between any two inbreds will always be the same. Once the inbreds that give a superior hybrid have been identified, the hybrid seed can be reproduced indefinitely as long as the homogeneity of the inbred parents is maintained. 
     The objective of commercial maize inbred line development programs is to develop new inbred lines that combine to produce high grain yields and superior agronomic performance in hybrid combination. The primary trait breeders seek is yield. However, other major agronomic traits are of importance in hybrid combination and have an impact on yield or otherwise provide superior performance in hybrid combinations. Such traits include percent grain moisture at harvest, relative maturity, resistance to stalk breakage, resistance to root lodging, grain quality, and disease and insect resistance. In addition the lines per se must have acceptable performance for parental traits such as seed yields, kernel sizes, pollen production, all of which affect ability to provide parental lines in sufficient quantity and quality for hybridization. Traits have been shown to be under genetic control and many if not all of the traits are affected by multiple genes. Thus, to be selected as an inbred line, the inbred must be able to combine such that the desired traits are passed to the hybrid and also be able to satisfy production requirements as a parental line. 
     Pedigree Breeding 
     The pedigree method of breeding is the mostly widely used methodology for new inbred line development. 
     In general terms this procedure consists of crossing two inbred lines to produce the non-segregating F 1  generation, and self pollination of the F 1  generation to produce the F 2  generation that segregates for all factors for which the inbred parents differ. An example of this process is set forth below. Variations of this generalized pedigree method are used, but all these variations produce a segregating generation which contains a range of variation for the traits of interest. 
     Example 1 
     Hypothetical Example of Pedigree Breeding Program 
     Consider a cross between two inbred lines that differ for alleles at five loci. 
     The parental genotypes are: 
     
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Parent 1        A b C d e F/A b C d e F                                   
Parent 2        a B c D E f/a B c D E f                                   
______________________________________                                    
 
    
     the F 1  from a cross between these two parents is: 
     
         ______________________________________                                    
F.sub.1        A b C d e F/a B c D E f                                    
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     Selfing F 1  will produce an F 2  generation including the following genotypes: 
     
         ______________________________________                                    
         A B c D E f/a b C d e F                                          
         A B c D e f/a b C d E F                                          
         A B c D e f/a b C d e F                                          
.                                                                         
.                                                                         
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     The number of genotypes in the F 2  is 3 6  for six segregating loci (729) and will produce (2 6 )-2 possible new inbreds, (62 for six segregating loci). 
     Each inbred parent which is used in breeding crosses represents a unique combination of genes, and the combined effects of the genes define the performance of the inbred and its performance in hybrid combination. There is published evidence (Smith, O. S., J. S. C. Smith, S. L. Bowen, R. A. Tenborg and S. J. Wall, TAG 80:833-840 (1990)) that each of these lines are different and can be uniquely identified on the basis of genetically-controlled molecular markers. 
     It has been shown (Hallauer, Arnel R. and Miranda, J. B. Fo. Quantitative Genetics in Maize Breeding, Iowa State University Press, Ames Iowa (1981)) that most traits of economic value in maize are under the genetic control of multiple genetic loci, and that there are a large number of unique combinations of these genes present in elite maize germplasm. If not, genetic progress using elite inbred lines would no longer be possible. Studies by Duvick and Russell (Duvick, D. N. Maydica 37:69-79 (1992); Russell, W. A. Maydica XXIX:375-390 (1983)) have shown that over the last 50 years the rate of genetic progress in commercial hybrids has been between 1 and 2% per year. 
     The number of genes affecting the trait of primary economic importance in maize, grain yield, has been estimated to be in the range of 10-1000. Inbred lines which are used as parents for breeding crosses differ in the number and combination of these genes. These factors make the plant breeder&#39;s task more difficult. Compounding this is evidence that no one line contains the favorable allele at all loci, and that different alleles have different economic values depending on the genetic background and field environment in which the hybrid is grown. Fifty years of breeding experience shows that there are many genes affecting grain yield and each of these has a relatively small effect on this trait. The effects are small compared to breeders&#39; ability to measure grain yield differences in evaluation trials. Therefore, the parents of the breeding cross must differ at several of these loci so that the genetic differences in the progeny will be large enough that breeders can develop a line that increases the economic worth of its hybrids over that of hybrids made with either parent. 
     If the number of loci segregating in a cross between two inbred lines is n, the number of unique genotypes in the F 2  generation is 3 n  (Example 2) and the number of unique inbred lines from this cross is {(2 n )-2}. Only a very limited number of these combinations are useful. Only about 1 in 10,000 of the progeny from F 2  &#39;s are commercially useful. 
     By way of example, if it is assumed that the number of segregating loci in F 2  is somewhere between 20 and 50, and that each parent is fixed for half the favorable alleles, it is then possible to calculate approximate probabilities of finding an inbred that has the favorable allele at {(n/2)+m} loci, where n/2 is the number of favorable alleles in each of the parents and m is the number of additional favorable alleles in the new inbred. See Example 2 below. The number m is assumed to be greater than three because each allele has so small an effect that evaluation techniques are not sensitive enough to detect differences due to three or less favorable alleles. The probabilities in Example 2 are on the order of 10 -5  or smaller and they are the probabilities that at least one genotype with (n/2)+m favorable alleles will exist. 
     To put this in perspective the number of plants grown on 60 million acres (approximate U.S. corn acreage) at 25000 plants/acre is 1.5×10 12 . 
     Example 2 
     Probability of Finding an Inbred with m of n Favorable Alleles 
     Assume each parent has n/2 of the favorable alleles and only  1/2 of the combinations of loci are economically useful. 
     
         ______________________________________                                    
no. of  no. favorable                                                     
                    no. additional                                        
                                 Probability                              
segregating                                                               
        alleles in  favorable alleles                                     
                                 that genotype                            
loci (n)                                                                  
        Parents (n/2)                                                     
                    in new inbred                                         
                                 occurs*                                  
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20      10          14           3 × 10.sup.-5                      
24      12          16           2 × 10.sup.-5                      
28      14          18           1 × 10.sup.-5                      
32      16          20           8 × 10.sup.-6                      
36      18          22           5 × 10.sup.-6                      
40      20          24           3 × 10.sup.-6                      
44      22          26           2 × 10.sup.-6                      
48      24          28           1 × 10.sup.-6                      
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 *Probability that a useful combination exists, does not include the      
 probability of identifying this combination if it does exist.            
 
    
     The possibility of having a usably high probability of being able to identify this genotype based on replicated field testing would be most likely smaller than this, and is a function of how large a population of genotypes is tested and how testing resources are allocated in the testing program. 
     At Pioneer Hi-Bred International, a typical corn research station has a staff of four, and 20 acres of breeding nursery. Those researchers plant those 20 acres with 25,000 nursery rows, 15,000 yield test plots in 10-15 yield test sites, and one or two disease-screening nurseries. Employing a temporary crew of 20 to 30 pollinators, the station makes about 65,000 hand pollinations per growing season. Thus, one of the largest plant breeding programs in the world does not have a sufficiently large breeding population to be able to rely upon &#34;playing the numbers&#34; to obtain successful research results. Nevertheless, Pioneer&#39;s breeders at each station produce from three to ten new inbreds which are proposed for commercial use each year. Over the 32 Pioneer research stations in North America, this amounts to from about 100 to 300 new inbreds proposed for use, and less than 50 and more commonly less than 30 of these inbreds that actually satisfy the performance criteria for commercial use. 
     This is a result of plant breeders using their skills, experience and intuitive ability to select inbreds having the necessary qualities. 
     SUMMARY OF THE INVENTION 
     According to the invention, there is provided a novel inbred corn line, designated PHHB4. This invention thus relates to the seeds of inbred corn line PHHB4, to the plants of inbred corn line PHHB4, and to methods for producing a corn plant produced by crossing the inbred line PHHB4 with itself or another corn line. This invention further relates to hybrid corn seeds and plants produced by crossing the inbred line PHHB4 with another corn line. 
     DEFINITIONS 
     In the description and examples that follow, a number of terms are used herein. In order to provide a clear and consistent understanding of the specification and claims, including the scope to be given such terms, the following definitions are provided. ABS is in absolute terms and % MN is percent of the mean for the experiments in which the inbred or hybrid was grown. 
     BAR PLT=BARREN PLANTS. The percent of plants per plot that were not barren (lack ears). 
     BRT STK=BRITTLE STALKS. This is a measure of the stalk breakage near the time of pollination, and is an indication of whether a hybrid or inbred would snap or break near the time of flowering under severe winds. Data are presented as percentage of plants that did not snap. 
     BU ACR=YIELD (BUSHELS/ACRE). Actual yield of the grain at harvest in bushels per acre adjusted to 15.5% moisture. 
     DRP EAR=DROPPED EARS. A measure of the number of dropped ears per plot and represents the percentage of plants that did not drop ears prior to harvest. 
     EAR HT=EAR HEIGHT. The ear height is a measure from the ground to the highest placed developed ear node attachment and is measured in inches. 
     EAR SZ=EAR SIZE. A 1 to 9 visual rating of ear size. The higher the rating the larger the ear size. 
     EST CNT=EARLY STAND COUNT. This is a measure of the stand establishment in the spring and represents the number of plants that emerge on a per plot basis for the inbred or hybrid. 
     GDU SHD=GDU TO SHED. The number of growing degree units (GDUs) or heat units required for an inbred line or hybrid to have approximately 50 percent of the plants shedding pollen and is measured from the time of planting. Growing degree units are calculated by the Barger Method, where the heat units for a 24-hour period are: ##EQU1## 
     The highest maximum temperature used is 86° F. and the lowest minimum temperature used is 50° F. For each inbred or hybrid it takes a certain number of GDUs to reach various stages of plant development. 
     GDU SLK=GDU TO SILK. The number of growing degree units required for an inbred line or hybrid to have approximately 50 percent of the plants with silk emergence from time of planting. Growing degree units are calculated by the Barger Method as given in GDU SHD definition. 
     GRN APP=GRAIN APPEARANCE. This is a 1 to 9 rating for the general appearance of the shelled grain as it is harvested based on such factors as the color of the harvested grain, any mold on the grain, and any cracked grain. High scores indicate good grain quality. 
     MST=HARVEST MOISTURE. The moisture is the actual percentage moisture of the grain at harvest. 
     PLT HT=PLANT HEIGHT. This is a measure of the height of the plant from the ground to the tip of the tassel in inches. 
     POL SC=POLLEN SCORE. A 1 to 9 visual rating indicating the amount of pollen shed. The higher the score the more pollen shed. 
     POL WT=POLLEN WEIGHT. This is calculated by dry weight of tassels collected as shedding commences minus dry weight from similar tassels harvested after shedding is complete. 
     It should be understood that the inbred can, through routine manipulation of cytoplasmic factors, be produced in a cytoplasmic male-sterile form which is otherwise phenotypically identical to the male-fertile form. 
     PRM=PREDICTED RM. This trait, predicted relative maturity (RM), is based on the harvest moisture of the grain. The relative maturity rating is based on a known set of checks and utilizes standard linear regression analyses and is referred to as the Comparative Relative Maturity Rating System which is similar to the Minnesota Relative Maturity Rating System. 
     RT LDG=ROOT LODGING. Root lodging is the percentage of plants that do not root lodge; plants that lean from the vertical axis at an approximately 30° angle or greater would be counted as root lodged. 
     SCT GRN=SCATTER GRAIN. A 1 to 9 visual rating indicating the amount of scatter grain (lack of pollination or kernel abortion) on the ear. The higher the score the less scatter grain. 
     SDG VGR=SEEDLING VIGOR. This is the visual rating (1 to 9) of the amount of vegetative growth after emergence at the seedling stage (approximately five leaves). A higher score indicates better vigor. 
     SEL IND=SELECTION INDEX. The selection index gives a single measure of the hybrid&#39;s worth based on information for up to five traits. A corn breeder may utilize his or her own set of traits for the selection index. One of the traits that is almost always included is yield. The selection index data presented in the tables represent the mean value averaged across testing stations. 
     STA GRN=STAY GREEN. Stay green is the measure of plant health near the time of black layer formation (physiological maturity). A high score indicates better late-season plant health. 
     STK CNT=NUMBER OF PLANTS. This is the final stand or number of plants per plot. 
     STK LDG=STALK LODGING. This is the percentage of plants that did not stalk lodge (stalk breakage) as measured by either natural lodging or pushing the stalks and determining the percentage of plants that break below the ear. 
     TAS BLS =TASSEL BLAST. A 1 to 9 visual rating was used to measure the degree of blasting (necrosis due to heat stress) of the tassel at time of flowering. A 1 would indicate a very high level of blasting at time of flowering, while a 9 would have no tassel blasting. 
     TAS SZ=TASSEL SIZE. A 1 to 9 visual rating was used to indicate the relative size of the tassel. The higher the rating the larger the tassel. 
     TAS WT=TASSEL WEIGHT. This is the average weight of a tassel (grams) just prior to pollen shed. 
     TEX EAR=EAR TEXTURE. A 1 to 9 visual rating was used to indicate the relative hardness (smoothness of crown) of mature grain. A 1 would be very soft (extreme dent) while a 9 would be very hard (flinty or very smooth crown). 
     TILLER=TILLERS. A count of the number of tillers per plot that could possibly shed pollen was taken. Data is given as percentage of tillers: number of tillers per plot divided by number of plants per plot. 
     TST WT=TEST WEIGHT (UNADJUSTED). The measure of the weight of the grain in pounds for a given volume (bushel). 
     TST WTA=TEST WEIGHT ADJUSTED. The measure of the weight of the grain in pounds for a given volume (bushel) adjusted for percent moisture. 
     YLD=YIELD. It is the same as BU ACR ABS. 
     YLD SC=YIELD SCORE. A 1 to 9 visual rating was used to give a relative rating for yield based on plot ear piles. The higher the rating the greater visual yield appearance. 
     MDM CPX=Maize Dwarf Mosaic Complex (MDMV=Maize Dwarf Mosaic Virus &amp; MCDV=Maize Chlorotic Dwarf Virus): Visual rating (1-9 score) where a &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. 
     SLF BLT=Southern Leaf Blight (Bipolaris maydis, Helminthosporium maydis): Visual rating (1-9 score) where a &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. 
     NLF BLT=Northern Leaf Blight (Exserohilum turcicum, H. turcicum): Visual rating (1-9 score) where a &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. 
     COM RST=Common Rust (Puccinia sorghi): Visual rating (1-9 score) where a &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. 
     GLF SPT=Gray Leaf Spot (Cercospora zeae-maydis): Visual rating (1-9 score) where a &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. 
     STW WLT=Stewart&#39;s Wilt (Erwinia stewartii): Visual rating (1-9 score) where a &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. 
     HD SMT=Head Smut (Sphacelotheca reiliana): Percentage of plants that did not have infection. 
     EAR MLD=General Ear Mold: Visual rating (1-9 score) where a &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. This is based on overall rating for ear mold of mature ears without determining specific mold organism, and may not be predictive for a specific ear mold. 
     ECB DPE=Dropped ears due to European Corn Borer (Ostrinia nubilalis): Percentage of plants that did not drop ears under second brood corn borer infestation. 
     ECB 2SC=European Corn Borer Second Brood (Ostrinia nubilalis): Visual rating (1-9 score) of post flowering damage due to infestation by European Corn Borer. A &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. 
     ECB 1LF=European Corn Borer First Brood (Ostrinia nubilalis): Visual rating (1-9 score) of pre-flowering leaf feeding by European Corn Borer. A &#34;1&#34; is very susceptible and a &#34;9&#34; is very resistant. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIGS. 1-3 show data for the trait Bushels Per Acre. The results of FIGS. 1-3 compare PHHB4 to PHW52, PHP38, and PHR61, respectively. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     PHHB4 brings together more yield in hybrids than either parental line. In hybrid combination PHHB4 has desirable plant and ear height, resistance to brittle stalk, and resistance to common rust. Hybrids of PHHB4 do well in both dry and wet years compared to the closest prior art, but are especially better in wet years. 
     Inbred corn line PHHB4 is a yellow, dent corn inbred and provides an acceptable female parental line in crosses for producing first generation F1 corn hybrids. PHHB4 is adapted to most regions of the United States but does best in Nebraska, Iowa, Illinois and Indiana. 
     The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. Most of the data in the Variety Description information was collected at Johnston, Iowa. The inbred has been self-pollinated and ear-rowed a sufficient number of generations with careful attention paid to uniformity of plant type to ensure homozygosity and phenotypic stability. The line has been increased both by hand and in isolated fields with continued observation for uniformity. No variant traits have been observed or are expected in PHHB4. 
     Inbred corn line PHHB4, being substantially homozygous, can be reproduced by planting seeds of the line, growing the resulting corn plants under self-pollinating or sib-pollinating conditions with adequate isolation, and harvesting the resulting seed, using techniques familiar to the agricultural arts. 
     
                       TABLE 1                                                     
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VARIETY DESCRIPTION INFORMATION                                           
INBRED = PHHB4                                                            
Type: Dent    Region Best Adapted: Most Regions                           
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A.   Maturity: Average across maturity zones. Zone: 0                     
     Heat Unit Shed: 1510                                                 
     Heat Unit Silk: 1540                                                 
     No. Reps: 50                                                         
      ##STR1##                                                            
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 *If maximum is greater than 86 degrees fahrenheit, then 86 is used and if
 minimum is less than 50, then 50 is used. Heat units accumulated daily an
 can not be less than 0.                                                  
 
    
     B. Plant Characteristics 
     Plant height (to tassel tip): 227 cm 
     Length of top ear internode: 11 cm 
     Number of ears per stalk: Single 
     Ear height (to base of top ear): 69 cm 
     Number of tillers: None 
     Cytoplasm type: Normal 
     C. Leaf 
     Color: (WF9) Medium Green 
     Angle from Stalk: 30-60 degrees 
     Marginal Waves: (HY) None 
     Number of Leaves (mature plants): 21 
     Sheath Pubescence: (W22) Light 
     Longitudinal Creases: (OH56A) Few 
     Length (Ear node leaf): 73 cm 
     Width (widest point, ear node leaf): 9 cm 
     D. Tassel 
     Number lateral branches: 1 
     Branch Angle from central spike: &gt;45 degrees 
     Pollen Shed: Light based on Pollen Yield Test (26% of experiment means) 
     Peduncle Length (top leaf to basal branches): 19 cm 
     Anther Color: Pink 
     Glume Color: Green 
     E. Ear (Husked Ear Data Except When Stated Otherwise) 
     Length: 16 cm 
     Weight: 151 gm 
     Mid-point Diameter: 48 mm 
     Silk Color: Green 
     Husk Extension (Harvest stage): Medium (barely covering ear) 
     Taper of Ear: Slight 
     Position of Shank (dry husks): Upright 
     Kernel Rows: Straight, Distinct Number=14 
     Husk Color (fresh): Light Green 
     Husk Color (dry): Buff 
     Shank Length: 11 cm 
     Shank (No. of internodes): 8 
     F. Kernel (Dried) 
     Size (from ear mid-point) 
     Length: 12 mm 
     Width: 9 mm 
     Thick: 5 mm 
     Shape Grade (% rounds): 40-60 (42% medium round based on Parent Test Data) 
     Pericarp Color: Colorless 
     Aleurone Color: Homozygous Yellow 
     Endosperm Color: Yellow 
     Endosperm Type: Normal Starch 
     Gm Wt/100 Seeds (unsized): 37 gm 
     G. Cob 
     Diameter at mid-point: 27 mm 
     Strength: Strong 
     Color: Red 
     H. Diseases 
     Corn Lethal Necrosis (MCMV=Maize Chlorotic Mottle Virus and MDMV=Maize Dwarf 
     Mosaic Virus): Intermediate 
     Maize Dwarf Mosaic Complex (MDMV &amp; MCDV=Maize Dwarf Virus): Susceptible 
     Anthracnose Stalk Rot ((C. graminicola): Intermediate 
     S. Leaf Blight (B. maydis): Resistant 
     Carbonum Leaf Blight (H. carbonum): Intermediate 
     N. Leaf Blight (E. turcicum): Intermediate 
     Common Rust (P. sorghi): Resistant 
     Gray Leaf Spot (C. zeae): Susceptible 
     Stewart&#39;s Wilt (E. stewartii): Resistant 
     Common Smut (U. maydis): Highly Resistant 
     Head Smut (S. reiliana): Highly Resistant 
     Fusarium Ear Mold (F. moniliforme): Intermediate 
     Gibberella Ear Rot (G. zeae): Intermediate 
     I. Insects 
     European Corn Borer-1 Leaf Damage (Pre-flowering): Susceptible 
     European Corn Borer-2 (Post-flowering): Susceptible 
     The above descriptions are based on a scale of 1-9, 1 being highly susceptible, 9 being highly resistant. 
     S (Susceptible): Would generally represent a score of 1-3. 
     I (Intermediate): Would generally represent a score of 4-5. 
     R (Resistant): Would generally represent a score of 6-7. 
     H (Highly Resistant): Would generally represent a score of 8-9. Highly resistant does not imply the inbred isimmune. 
     
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J. Variety Most Closely Resembling:                                       
       Character                                                          
               Inbred                                                     
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       Maturity                                                           
               PHW52                                                      
       Usage   PHW52                                                      
______________________________________                                    
 PHW52(PVP Certificate No. 8800215) is a Pioneer HiBred International, Inc
 proprietary inbred.                                                      
 
    
     Data for Items B, C, D, E, F, and G is based primarily on a maximum of two reps from Johnston, Iowa grown in 1992, plus description information from the maintaining station. 
     ELECTROPHORESIS RESULTS 
     Isozyme Genotypes for PHHB4 
     Isozyme data were generated for inbred corn line PHHB4 according to the procedures described in Stuber, C. W., Wendel, J. F., Goodman, M. M., and Smith, J. S. C., &#34;Techniques and Scoring Procedures for Starch Gel Electrophoresis of Enzymes from Maize (Zea mays L.)&#34;, Technical Bulletin No. 286, North Carolina Agricultural Research Service, North Carolina State University, Raleigh, N.C. (1988). 
     The data in Table 2 compares PHHB4 with its parents, PHW52 and PHV94. 
     
                       TABLE 2                                                     
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ELECTROPHORESIS RESULTS FOR PHHB4                                         
AND ITS PARENTS PHW52 AND PHV94                                           
                 PARENTS                                                  
LOCI     PHHB4         PHW52    PHV94                                     
______________________________________                                    
ACP1     2             2        4                                         
ADH1     4             4        4                                         
CAT3     9             9        9                                         
DIA1     8             8        8                                         
GOT1     4             4        4                                         
GOT2     2             2        4                                         
GOT3     4             4        4                                         
IDH1     4             4        4                                         
IDH2     6             6        6                                         
MDH1     6             6        6                                         
MDH2     6             6        6                                         
MDH3     16            16       16                                        
MDH4     12            12       12                                        
MDH5     12            12       12                                        
MMM      4             4        4                                         
PGM1     9             9        9                                         
PGM2     4             4        4                                         
PGD1     2             2        2                                         
PGD2     5             5        5                                         
PHI1     4             5        4                                         
______________________________________                                    
 
    
     Examples 
     INBRED AND HYBRID PERFORMANCE OF PHHB4 
     In the examples that follow, the traits and characteristics of inbred corn line PHHB4 are given as a line in comparison with other inbreds and in hybrid combination. The data collected on inbred corn line PHHB4 is presented for the key characteristics and traits. 
     Table 3A compares PHHB4 to PHW52. PHHB4 has lower yield and grain harvest moisture but higher test weight than PHW52. PHHB4 is a taller inbred with higher ear placement compared to PHW52. PHHB4 has better seedling vigor and sheds (GDU Shed) later than PHW52. PHHB4 has good ear texture but more scattergrain compared to PHW52. PHHB4 has better resistance to Stewart&#39;s wilt and first brood European corn borer than PHW52. 
     The data in Table 3B shows PHHB4 and PHP38 have similar yield and test weight but PHHB4 has lower grain harvest moisture. PHHB4 has a larger ear and is taller with lower ear placement PHP38. PHHB4 flowers (GDU Shed and GDU Silk) later than PHP38. PHHB4 has better Stewart&#39;s wilt resistance than PHP38. 
     Table 3C compares PHHB4 to PHR61. The data shows PHHB4 has higher yield and lower test weight than PHR61. PHHB4 has a larger ear and is a shorter inbred with lower ear placement than PHR61. PHHB4 flowers later (GDU Shed and GDU Silk) than PHR61. PHHB4 has better staygreen than PHR61. 
     Table 4A compares PHHB4 to PHW52 when both were crossed to the same inbred testers. The PHHB4 hybrids have higher yield and test weight but lower grain harvest moisture compared to the PHW52 hybrids. The hybrids have similar ear placement but the PHHB4 hybrids are taller. 
     Table 4B compares PHHB4 to PHP38 when both were crossed to the same inbred testers. The PHHB4 hybrids have higher yield than the PHP38 hybrids. The PHHB4 hybrids shed (GDU Shed) later than the PHP38 hybrids. 
     Table 4C compares PHHB4 to PHR61 when both were crossed to the same inbred testers. The PHHB4 hybrids have higher yield and grain harvest moisture compared to the PHR61 hybrids. The PHHB4 hybrids shed (GDU Shed) later than the PHR61 hybrids. The PHHB4 hybrids have better grain appearance and are taller with lower ear placement compared to the PHR61 hybrids. 
     Table 5A compares PHHB4 to PHW52 when both were crossed to the same inbred. The data shows the PHHB4 hybrid is higher yielding with lower grain harvest moisture compared to the PHW52 hybrid. The PHHB4 hybrid has better test weight and grain appearance than the PHW52 hybrid. The PHHB4 hybrid is taller with higher ear placement and sheds (GDU Shed) later than the PHW52 hybrid. 
     Table 5B compares PHHB4 to PHR61 when both were crossed to the same inbred. The PHHB4 hybrid has higher yield and grain harvest moisture compared to the PHR61 hybrid. The hybrids have similar plant height but the PHHB4 hybrid has lower ear placement than the PHR61 hybrid. The PHHB4 hybrid sheds (GDU Shed) later than the PHR61 hybrid. 
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 compares the yield of PHHB4 to PHW52. PHHB4 is lower yielding than PHW52 and has below average yield in low yield environments. 
     FIG. 2 compares the yield of PHHB4 and PHP38. PHHB4 has lower yield across all environments compared to PHP38. 
     FIG. 3 compares the yield of PHHB4 and PHR61. PHHB4 has below average yield except in the most extreme high yield environments. Compared to PHR61, PHHB4 is higher yielding in high yield environments but lower yielding in low yield environments. 
     
                                           TABLE 3A                                
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PAIRED INBRED COMPARISON DATA                                             
VARIETY #1 - PHHB4                                                        
VARIETY #2 - PHW52                                                        
__________________________________________________________________________
        BU BU  YLD     EAR                                                
                          BAR                                             
                             PLT EAR SDG EST DRP                          
                                                TIL                       
                                                   GDU GDU POL            
    VAR ACR                                                               
           ACR SC  MST SZ PLT                                             
                             HT  HT  VGR CNT EAR                          
                                                LER                       
                                                   SHD SLK SC             
DEPT                                                                      
    #   ABS                                                               
           % MN                                                           
               ABS ABS ABS                                                
                          ABS                                             
                             ABS ABS ABS ABS ABS                          
                                                ABS                       
                                                   ABS ABS ABS            
__________________________________________________________________________
TO- 1   76.3                                                              
           96  5.5 17.9                                                   
                       5.8                                                
                          92.0                                            
                             84.5                                         
                                 28.3                                     
                                     5.5 37.3                             
                                             99.5                         
                                                0.8                       
                                                   1451                   
                                                       1480               
                                                           2.4            
TAL 2   82.9                                                              
           106 6.3 21.2                                                   
                       5.8                                                
                          93.9                                            
                             75.6                                         
                                 26.3                                     
                                     4.9 39.7                             
                                             99.9                         
                                                0.9                       
                                                   1441                   
                                                       1479               
                                                           6.2            
SUM LOCS                                                                  
        30 30  19  43  17 57 53  53  55  78  8  54 55  52  18             
    REPS                                                                  
        78 78  20  92  17 90 92  88  82  145 16 80 65  57  20             
    DIFF                                                                  
        6.6                                                               
           10  0.8 3.4 0.1                                                
                          1.9                                             
                             8.9 2.1 0.6 2.3 0.4                          
                                                0.2                       
                                                   10  01  3.8            
    PROB                                                                  
        .098*                                                             
           .093*                                                          
               .028+                                                      
                   .000#                                                  
                       .842                                               
                          .204                                            
                             .000#                                        
                                 .000#                                    
                                     .003#                                
                                         .000#                            
                                             .209                         
                                                .599                      
                                                   0.55*                  
                                                       .922               
                                                           .000#          
__________________________________________________________________________
           TAS                                                            
              TAS TEX TST GRN SCT STA STK RT  EAR NLF                     
                                                     STW ECB              
                                                            ECB           
       VAR BLS                                                            
              SZ  EAR WT  APP GRN GRN LDG LDG MLD BLT                     
                                                     WLT 1LF              
                                                            2SC           
DEPT   #   ABS                                                            
              ABS ABS ABS ABS ABS ABS ABS ABS ABS ABS                     
                                                     ABS ABS              
                                                            ABS           
__________________________________________________________________________
TOTAL  1   9.0                                                            
              2.8 6.6 58.8                                                
                          6.1 5.6 5.2 94.8                                
                                          98.2                            
                                              7.4 4.3                     
                                                     5.8 3.8              
                                                            4.6           
SUM    2   9.0                                                            
              6.0 5.9 57.0                                                
                          5.6 6.8 5.7 89.0                                
                                          98.1                            
                                              7.5 3.9                     
                                                     4.0 3.1              
                                                            4.1           
       LOCS                                                               
           1  21  14  28  14  19  32  23  14  16  13 4   19 8             
       REPS                                                               
           1  23  14  72  27  20  53  51  25  17  20 4   24 14            
       DIFF                                                               
           0.0                                                            
              3.2 0.7 1.8 0.5 1.2 0.5 5.8 0.1 0.1 0.4                     
                                                     1.8 0.7              
                                                            0.4           
       PROB   .000#                                                       
                  .035+                                                   
                      .000#                                               
                          .312                                            
                              .017+                                       
                                  .153                                    
                                      .012+                               
                                          .883                            
                                              .872                        
                                                  .293                    
                                                     .006#                
                                                         .062*            
                                                            .514          
__________________________________________________________________________
 * = 10% SIG                                                              
 + = 5% SIG                                                               
 # = 1% SIG                                                               
 
    
     
                                           TABLE 3B                                
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA                                             
VARIETY #1 - PHHB4                                                        
VARIETY #2 - PHP38                                                        
__________________________________________________________________________
        BU BU  YLD     EAR                                                
                          BAR                                             
                             PLT EAR SDG EST DRP                          
                                                TIL                       
                                                   GDU GDU POL            
    VAR ACR                                                               
           ACR SC  MST SZ PLT                                             
                             HT  HT  VGR CNT EAR                          
                                                LER                       
                                                   SHD SLK SC             
DEPT                                                                      
    #   ABS                                                               
           % MN                                                           
               ABS ABS ABS                                                
                          ABS                                             
                             ABS ABS ABS ABS ABS                          
                                                ABS                       
                                                   ABS ABS ABS            
__________________________________________________________________________
TO- 1   75.3                                                              
           95  5.4 17.5                                                   
                       5.8                                                
                          92.2                                            
                             84.5                                         
                                 28.3                                     
                                     5.5 37.8                             
                                             99.5                         
                                                0.7                       
                                                   1450                   
                                                       1479               
                                                           2.5            
TAL 2   77.1                                                              
           99  5.3 18.7                                                   
                       5.2                                                
                          91.6                                            
                             81.2                                         
                                 29.3                                     
                                     5.8 40.0                             
                                             99.2                         
                                                1.1                       
                                                   1426                   
                                                       1440               
                                                           5.4            
SUM LOCS                                                                  
        33 33  20  47  17 60 53  53  55  83  8  58 60  57  22             
    REPS                                                                  
        66 66  21  80  19 81 81  81  75  132 16 79 68  62  24             
    DIFF                                                                  
        1.8                                                               
           4   0.1 1.2 0.6                                                
                          0.6                                             
                             3.3 1.0 0.3 2.1 0.3                          
                                                0.3                       
                                                   24  39  2.9            
    PROB                                                                  
        .599                                                              
           .487                                                           
               .785                                                       
                   .000#                                                  
                       .096*                                              
                          .657                                            
                             .000#                                        
                                 .050*                                    
                                     .113                                 
                                         .002#                            
                                             .406                         
                                                .325                      
                                                   .000#                  
                                                       .000#              
                                                           .000#          
__________________________________________________________________________
           TAS                                                            
              TAS TEX TST GRN SCT STA STK RT  EAR NLF                     
                                                     STW ECB              
                                                            ECB           
       VAR BLS                                                            
              SZ  EAR WT  APP GRN GRN LDG LDG MLD BLT                     
                                                     WLT 1LF              
                                                            2SC           
DEPT   #   ABS                                                            
              ABS ABS ABS ABS ABS ABS ABS ABS ABS ABS                     
                                                     ABS ABS              
                                                            ABS           
__________________________________________________________________________
TOTAL  1   9.0                                                            
              2.8 6.6 58.6                                                
                          6.1 5.7 5.2 94.8                                
                                          98.2                            
                                              7.4 4.3                     
                                                     5.8 3.9              
                                                            5.0           
SUM    2   9.0                                                            
              5.2 6.9 58.8                                                
                          6.6 6.0 5.8 93.7                                
                                          99.1                            
                                              8.0 4.2                     
                                                     3.8 3.5              
                                                            4.9           
       LOCS                                                               
           1  22  14  31  15  20  32  23  14  16  13 4   20 7             
       REPS                                                               
           1  24  14  62  29  21  48  44  25  17  20 4   25 11            
       DIFF                                                               
           0.0                                                            
              2.4 0.3 0.2 0.5 0.3 0.7 1.0 0.8 0.6 0.0                     
                                                     2.0 0.4              
                                                            0.1           
       PROB   .000#                                                       
                  .414                                                    
                      .463                                                
                          .056*                                           
                              .406                                        
                                  .096*                                   
                                      .136                                
                                          .444                            
                                              .132                        
                                                  .886                    
                                                     .000#                
                                                         .159             
                                                            .818          
__________________________________________________________________________
 * = 10% SIG                                                              
 + = 5% SIG                                                               
 # = 1% SIG                                                               
 
    
     
                                           TABLE 3C                                
__________________________________________________________________________
PAIRED INBRED COMPARISON DATA                                             
VARIETY #1 - PHHB4                                                        
VARIETY #2 - PHR61                                                        
__________________________________________________________________________
        BU BU  YLD     EAR                                                
                          BAR                                             
                             PLT EAR SDG EST DRP                          
                                                TIL                       
                                                   GDU GDU POL            
    VAR ACR                                                               
           ACR SC  MST SZ PLT                                             
                             HT  HT  VGR CNT EAR                          
                                                LER                       
                                                   SHD SLK SC             
DEPT                                                                      
    #   ABS                                                               
           % MN                                                           
               ABS ABS ABS                                                
                          ABS                                             
                             ABS ABS ABS ABS ABS                          
                                                ABS                       
                                                   ABS ABS ABS            
__________________________________________________________________________
TO- 1   84.2                                                              
           98  5.4 17.4                                                   
                       5.5                                                
                          92.8                                            
                             86.6                                         
                                 28.5                                     
                                     5.3 34.6                             
                                             100.0                        
                                                0.8                       
                                                   1439                   
                                                       1470               
                                                           2.4            
TAL 2   77.0                                                              
           92  5.1 17.0                                                   
                       4.7                                                
                          91.8                                            
                             91.2                                         
                                 32.7                                     
                                     5.6 39.1                             
                                             100.0                        
                                                1.1                       
                                                   1420                   
                                                       1431               
                                                           2.5            
SUM LOCS                                                                  
        17 17  14  31  12 43 33  33  38  60  2  42 47  46  17             
    REPS                                                                  
        34 34  15  48  12 54 49  49  48  96  4  53 52  50  19             
    DIFF                                                                  
        7.2                                                               
           6   0.2 0.4 0.8                                                
                          1.1                                             
                             4.7 4.2 0.3 4.6 0.0                          
                                                0.3                       
                                                   19  39  0.1            
    PROB                                                                  
        .119                                                              
           .310                                                           
               .701                                                       
                   .138                                                   
                       .096*                                              
                          .608                                            
                             .000#                                        
                                 .000#                                    
                                     .159                                 
                                         .000#                            
                                             1.00                         
                                                .447                      
                                                   .003#                  
                                                       .000#              
                                                           .835           
__________________________________________________________________________
          TAS                                                             
             TAS TEX TST GRN SCT STA STK RT  EAR NLF STW ECB              
                                                            ECB           
      VAR BLS                                                             
             SZ  EAR WT  APP GRN GRN LDG LDG MLD BLT WLT 1LF              
                                                            2SC           
DEPT  #   ABS                                                             
             ABS ABS ABS ABS ABS ABS ABS ABS ABS ABS ABS ABS              
                                                            ABS           
__________________________________________________________________________
TOTAL 1   9.0                                                             
             2.7 6.7 57.1                                                 
                         5.6 5.4 5.5 94.4                                 
                                         98.4                             
                                             8.0 4.3 5.7 3.6              
                                                            3.7           
SUM   2   9.0                                                             
             1.8 7.3 59.0                                                 
                         6.6 6.7 4.1 91.8                                 
                                         77.1                             
                                             8.1 5.3 3.3 3.7              
                                                            5.7           
      LOCS                                                                
          1  14  10  15  8   15  18  11  4   12  11  3   14 3             
      REPS                                                                
          1  16  10  30  15  16  26  21  7   13  16  3   17 3             
      DIFF                                                                
          0.0                                                             
             0.9 0.6 2.0 0.9 1.3 1.4 2.5 21.3                             
                                             0.1 1.0 2.3 0.1              
                                                            2.0           
      PROB   .017+                                                        
                 .051*                                                    
                     .000#                                                
                         .059*                                            
                             .014+                                        
                                 .009#                                    
                                     .597                                 
                                         .405                             
                                             .838                         
                                                 .026+                    
                                                     .222                 
                                                         .753             
                                                            .074*         
__________________________________________________________________________
 * = 10% SIG                                                              
 + = 5% SIG                                                               
 # = 1% SIG                                                               
 
    
     
                                           TABLE 4A                                
__________________________________________________________________________
AVERAGE INBRED BY TESTER PERFORMANCE COMPARING PHW52 TO PHHB4             
CROSSED TO THE SAME INBRED TESTERS AND GROWN IN THE SAME                  
EXPERIMENTS. ALL VALUES ARE EXPRESSED AS PERCENT OF THE EXPERIMENT        
MEAN EXCEPT PREDICTED RM, SELECTION INDEX, AND YIELD (BU/ACR).            
__________________________________________________________________________
                 SEL                                                      
                    BU       GDU PRM STK RT  STA                          
       INBRED                                                             
             PRM IND                                                      
                    ACR                                                   
                       YLD                                                
                          MST                                             
                             SHD SHD LDG LDG GRN                          
__________________________________________________________________________
TOTAL  REPLIC.                                                            
             65  64 64 64 65 21  8   53  17  29                           
MEAN WTS                                                                  
       PHW52 112 95 181                                                   
                       98 104                                             
                             99  108 101 102 106                          
MEAN WTS                                                                  
       PHHB4 110 103                                                      
                    188                                                   
                       101                                                
                          99 99  108 100 93  100                          
       DIFF. 2   9  7  4  5  0   0   1   9   6                            
__________________________________________________________________________
                   TST GRN SDG EST                                        
                                  STK                                     
                                     PLT                                  
                                        EAR                               
                                           DRP                            
                                              BRT                         
             INBRED                                                       
                   WTA APP VGR CNT                                        
                                  CNT                                     
                                     HT HT EAR                            
                                              STK                         
__________________________________________________________________________
TOTAL        REPLIC.                                                      
                   49  23  39  34 65 36 36 10 7                           
MEAN WTS     PHW52 100 95  100 101                                        
                                  100                                     
                                     97 96 100                            
                                              100                         
MEAN WTS     PHHB4 102 99  107 100                                        
                                  102                                     
                                     100                                  
                                        96 100                            
                                              102                         
             DIFF. 1   4   7   1  1  2  0  0  2                           
__________________________________________________________________________
 
    
     
                                           TABLE 4B                                
__________________________________________________________________________
AVERAGE INBRED BY TESTER PERFORMANCE COMPARING PHP38 TO PHHB4             
CROSSED TO THE SAME INBRED TESTERS AND GROWN IN THE SAME                  
EXPERIMENTS. ALL VALUES ARE EXPRESSED AS PERCENT OF THE EXPERIMENT        
MEAN EXCEPT PREDICTED RM, SELECTION INDEX, AND YIELD (BU/ACR).            
__________________________________________________________________________
                 SEL                                                      
                    BU       GDU PRM STK RT  STA                          
       INBRED                                                             
             PRM IND                                                      
                    ACR                                                   
                       YLD                                                
                          MST                                             
                             SHD SHD LDG LDG GRN                          
__________________________________________________________________________
TOTAL  REPLIC.                                                            
             132 137                                                      
                    137                                                   
                       137                                                
                          140                                             
                             50  12  113 46  55                           
MEAN WTS                                                                  
       PHP38 110 97 178                                                   
                       98 99 99  109 102 102 110                          
MEAN WTS                                                                  
       PHHB4 111 104                                                      
                    185                                                   
                       102                                                
                          99 101 111 100 100 109                          
       DIFF.     6  7  3  1  2   2   2   2   1                            
__________________________________________________________________________
                   TST GRN SDG EST                                        
                                  STK                                     
                                     PLT                                  
                                        EAR                               
                                           DRP                            
                                              BRT                         
             INBRED                                                       
                   WTA APP VGR CNT                                        
                                  CNT                                     
                                     HT HT EAR                            
                                              STK                         
__________________________________________________________________________
TOTAL        REPLIC.                                                      
                   115 54  69  73 147                                     
                                     82 82 37 20                          
MEAN WTS     PHP38 101 105 105 99 100                                     
                                     96 96 100                            
                                              102                         
MEAN WTS     PHHB4 101 99  104 98 99 101                                  
                                        95 100                            
                                              102                         
             DIFF. 1   6   1   2  1  4  1  0  0                           
__________________________________________________________________________
 
    
     
                                           TABLE 4C                                
__________________________________________________________________________
AVERAGE INBRED BY TESTER PERFORMANCE COMPARING PHR61 TO PHHB4             
CROSSED TO THE SAME INBRED TESTERS AND GROWN IN THE SAME                  
EXPERIMENTS. ALL VALUES ARE EXPRESSED AS PERCENT OF THE EXPERIMENT        
MEAN EXCEPT PREDICTED RM, SELECTION INDEX, AND YIELD (BU/ACR).            
__________________________________________________________________________
                 SEL                                                      
                    BU       GDU PRM STK RT  STA                          
       INBRED                                                             
             PRM IND                                                      
                    ACR                                                   
                       YLD                                                
                          MST                                             
                             SHD SHD LDG LDG GRN                          
__________________________________________________________________________
TOTAL  REPLIC.                                                            
             20  20 20 20 20 10  2   16  8   10                           
MEAN WTS                                                                  
       PHR61 106 86 164                                                   
                       92 95 99  109 100 90  107                          
MEAN WTS                                                                  
       PHHB4 108 102                                                      
                    182                                                   
                       102                                                
                          103                                             
                             101 110 99  109 108                          
       DIFF. 2   16 18 10 8  1   1   1   19  1                            
__________________________________________________________________________
                      TST GRN SDG EST                                     
                                     STK                                  
                                        PLT                               
                                           EAR                            
                                              BRT                         
                INBRED                                                    
                      WTA APP VGR CNT                                     
                                     CNT                                  
                                        HT HT STK                         
__________________________________________________________________________
         TOTAL  REPLIC.                                                   
                      18  10  8   7  22 10 10 2                           
         MEAN WTS                                                         
                PHR61 103 109 90  100                                     
                                     102                                  
                                        101                               
                                           100                            
                                              106                         
         MEAN WTS                                                         
                PHHB4 102 113 99  96 100                                  
                                        104                               
                                           98 105                         
                DIFF. 1   4   9   4  2  2  2  1                           
__________________________________________________________________________
 
    
     
                                           TABLE 5A                                
__________________________________________________________________________
VARIETY #1 - PHHB4 HYBRID                                                 
VARIETY #2 - PHW52 HYBRID                                                 
__________________________________________________________________________
                    BU  BU      STK RT  STA TST                           
        VAR     PRM ACR ACR MST LDG LDG GRN WTA                           
DEPT    #   PRM SHD ABS % MN                                              
                            % MN                                          
                                % MN                                      
                                    % MN                                  
                                        % MN                              
                                            % MN                          
__________________________________________________________________________
TOTAL SUM                                                                 
        1   107 109 178.8                                                 
                        105 96  98  100 88  100                           
        2   109 107 172.1                                                 
                        102 101 99  104 105 99                            
        LOCS                                                              
            30  18  133 133 136 115 53  55  126                           
        REPS                                                              
            30  18  174 174 177 145 74  69  159                           
        DIFF                                                              
            2   1   6.7 4   5   2   3   18  0                             
        PROB                                                              
            .000#                                                         
                .001#                                                     
                    .000#                                                 
                        .001#                                             
                            .000#                                         
                                .103                                      
                                    .023+                                 
                                        .000#                             
                                            .061*                         
__________________________________________________________________________
            GRN SDG EST STK PLT EAR DRP BRT GDU                           
        VAR APP VGR CNT CNT HT  HT  EAR STK SHD                           
DEPT    #   % MN                                                          
                % MN                                                      
                    % MN                                                  
                        % MN                                              
                            % MN                                          
                                % MN                                      
                                    % MN                                  
                                        % MN                              
                                            % MN                          
__________________________________________________________________________
TOTAL SUM                                                                 
        1   102 103 98  100 103 92  100 102 100                           
        2   91  103 100 100 95  85  100 102 98                            
        LOCS                                                              
            45  51  87  145 70  70  50  7   50                            
        REPS                                                              
            55  72  107 189 82  82  68  10  60                            
        DIFF                                                              
            11  0   2   0   8   7   0   0   2                             
        PROB                                                              
            .000#                                                         
                .940                                                      
                    .064*                                                 
                        .684                                              
                            .000#                                         
                                .000#                                     
                                    .170                                  
                                        .885                              
                                            .000#                         
__________________________________________________________________________
 * = 10% SIG                                                              
 + = 5% SIG                                                               
 # = 1% SIG                                                               
 
    
     
                                           TABLE 5B                                
__________________________________________________________________________
VARIETY #1 - PHHB4 HYBRID                                                 
VARIETY #2 - PHR61 HYBRID                                                 
__________________________________________________________________________
                    BU  BU      STK RT  STA TST                           
        VAR     PRM ACR ACR MST LDG LDG GRN WTA                           
DEPT    #   PRM SHD ABS % MN                                              
                            % MN                                          
                                % MN                                      
                                    % MN                                  
                                        % MN                              
                                            % MN                          
__________________________________________________________________________
TOTAL SUM                                                                 
        1   107 108 178.7                                                 
                        105 97  98  100 90  100                           
        2   103 107 165.0                                                 
                        98  88  100 103 85  102                           
        LOCS                                                              
            23  15  105 105 105 87  43  43  95                            
        REPS                                                              
            23  15  137 137 137 110 58  54  119                           
        DIFF                                                              
            4   1   13.7                                                  
                        8   9   2   3   4   2                             
        PROB                                                              
            .000#                                                         
                .005#                                                     
                    .000#                                                 
                        .000#                                             
                            .000#                                         
                                .175                                      
                                    .363                                  
                                        .512                              
                                            .000#                         
__________________________________________________________________________
            GRN SDG EST STK PLT EAR DRP BRT GDU                           
        VAR APP VGR CNT CNT HT  HT  EAR STK SHD                           
DEPT    #   % MN                                                          
                % MN                                                      
                    % MN                                                  
                        % MN                                              
                            % MN                                          
                                % MN                                      
                                    % MN                                  
                                        % MN                              
                                            % MN                          
__________________________________________________________________________
TOTAL SUM                                                                 
        1   103 100 98  100 104 93  100 102 101                           
        2   107 103 102 101 103 97  100 102 100                           
        LOCS                                                              
            41  36  64  107 49  49  34  7   33                            
        REPS                                                              
            50  53  80  142 56  56  47  10  38                            
        DIFF                                                              
            4   4   4   1   1   4   0   0   1                             
        PROB                                                              
            .137                                                          
                .415                                                      
                    .000#                                                 
                        .220                                              
                            .338                                          
                                .013+                                     
                                    .243                                  
                                        .939                              
                                            .000#                         
__________________________________________________________________________
 * = 10% SIG                                                              
 + = 5% SIG                                                               
 # = 1% SIG                                                               
 
    
     INDUSTRIAL APPLICABILITY 
     The foregoing is set forth by way of example and is not intended to limit the scope of the invention. 
     This invention also is directed to methods for producing a corn plant by crossing a first parent corn plant with a second parent corn plant wherein the first or second parent corn plant is an inbred corn plant from the line PHHB4. Further, both first and second parent corn plants can come from the inbred corn line PHHB4. Thus, any such methods using the inbred corn line PHHB4 are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, and the like. It also would include more unconventional methods of combining the inbred with another such as using various culturing techniques known to those skilled in the art. All plants produced using inbred corn line PHHB4 as a parent are within the scope of this invention. Advantageously, the inbred corn line is used in crosses with other, different, corn inbreds to produce first generation (F 1 ) corn hybrid seeds and plants with superior characteristics. 
     As used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which corn plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like. 
     Duncan, Williams, Zehr, and Widholm, Planta, (1985) 165:322-332 reflects that 97% of the plants cultured which produced callus were capable of plant regeneration. Subsequent experiments with both inbreds and hybrids produced 91% regenerable callus which produced plants. In a further study in 1988, Songstad, Duncan &amp; Widholm in Plant Cell Reports (1988), 7:262-265 reports several media additions which enhance regenerability of callus of two inbred lines. Other published reports also indicated that &#34;nontraditional&#34; tissues are capable of producing somatic embryogenesis and plant regeneration. K. P. Rao, et al., Maize Genetics Cooperation Newsletter, 60:64-65 (1986), refers to somatic embryogenesis from glume callus cultures and B. V. Conger, et al., Plant Cell Reports, 6:345-347 (1987) indicates somatic embryogenesis from the tissue cultures of maize leaf segments. Thus, it is clear from the literature that the state of the art is such that these methods of obtaining plants are, and were, &#34;conventional&#34; in the sense that they are routinely used and have a very high rate of success. 
     Tissue culture of corn is described in European Patent Application, publication 160,390, incorporated herein by reference. Corn tissue culture procedures are also described in Green and Rhodes, &#34;Plant Regeneration in Tissue Culture of Maize,&#34; Maize for Biological Research (Plant Molecular Biology Association, Charlottesville, Va. 1982, at 367-372) and in Duncan, et al., &#34;The Production of Callus Capable of Plant Regeneration from Immature Embryos of Numerous Zea Mays Genotypes,&#34; 165 Planta 322-332 (1985). Thus, another aspect of this invention is to provide cells which upon growth and differentiation produce corn plants having the physiological and morphological characteristics of the inbred line PHHB4. 
     Corn is used as human food, livestock feed, and as raw material in industry. The food uses of corn, in addition to human consumption of corn kernels, include both products of dry- and wet-milling industries. The principal products of corn dry milling are grits, meal and flour. The corn wet-milling industry can provide corn starch, corn syrups, and dextrose for food use. Corn oil is recovered from corn germ, which is a by-product of both dry- and wet-milling industries. 
     Corn, including both grain and non-grain portions of the plant, is also used extensively as livestock feed, primarily for beef cattle, dairy cattle, hogs, and poultry. 
     Industrial uses of corn are mainly from corn starch in the wet-milling industry and corn flour in the dry-milling industry. The industrial applications of corn starch and flour are based on functional properties, such as viscosity, film formation, adhesive properties, and ability to suspend particles. The corn starch and flour have application in the paper and textile industries. Other industrial uses include applications in adhesives, building materials, foundry binders, laundry starches, explosives, oil-well muds, and other mining applications. 
     Plant parts other than the grain of corn are also used in industry. Stalks and husks are made into paper and wallboard and cobs are used for fuel and to make charcoal. 
     The seed of inbred corn line PHHB4, the plant produced from the inbred seed, the hybrid corn plant produced from the crossing of the inbred, hybrid seed, and various parts of the hybrid corn plant can be utilized for human food, livestock feed, and as a raw material in industry. 
     Although the foregoing invention has been described in some detail by way of illustration and example for the purposes of clarity and understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the invention, as limited only by the scope of the appended claims. 
     Deposits 
     Applicant has made a deposit of at least 2500 seeds of Inbred Corn Line PHHB4 with the American Type Culture Collection (ATCC), Rockville, Md. 20852 USA, ATCC Deposit No. 97048. The seeds deposited with the ATCC on Feb. 9, 1995 were taken from the deposit maintained by Pioneer Hi-Bred International, Inc., 700 Capital Square, 400 Locust Street, Des Moines, Iowa 50309-2340 since prior to the filing date of this application. The deposit of the Inbred Corn Line PHHB4 will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the enforceable life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Additionally, Applicant has satisfied all the requirements of 27 C.F.R. §§1.801-1.809, including providing an indication of the viability of the sample. Applicant imposes no restrictions on the availability of the deposited material from the ATCC; however, Applicant has no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicant does not waive any infringement of its rights granted under this patent or under the Plant Variety Protection Act (7 USC 2312 et seq.). PHHB4 is a U.S. protected variety under Plant Variety Protection Certificate 9400093.