Patent Publication Number: US-2021170404-A1

Title: Test container for examination

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     The present application claims priority under 35 U.S.C. § 119 to Japanese Patent Application No. 2019-222329 filed on Dec. 9, 2019. The above application is hereby expressly incorporated by reference, in its entirety, into the present application. 
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The technology of the present disclosure relates to a test container. 
     2. Description of the Related Art 
     Test containers such as a test cartridge, an analysis chip, and the like used for performing various analyses with respect to a specimen extracted from a biological sample are known. 
     JP2007-101428A discloses a cartridge for a chemical treatment having a plurality of wells (liquid accommodation portions) accommodating a liquid and configured by stacking an elastic member having a plurality of recesses on one surface on a substrate so that the recesses face the substrate side, and a flow path connecting between the wells. JP2007-101428A discloses a method for rotating a roller while pressing the elastic member of a cartridge for elastic deformation of the elastic member, to cause pressing of a liquid in the elastically deformed well to move to an adjacent well via the flow path connected to the well. 
     JP2003-166910A discloses a liquid feeding mechanism which feeds a liquid filled in a liquid tank to a flow path connected to the liquid tank by changing a volume of the liquid tank (liquid accommodation portion) formed to surround a wall, and an analysis device having the liquid feeding mechanism. 
     SUMMARY OF THE INVENTION 
     However, in JP2007-101428A and JP2003-166910A, a liquid is fed by deforming an elastic member, but there is no specific description about physical properties suitable as the elastic member. In addition, in the test container disclosed in both JP2007-101428A and JP2003-166910A, the flow path connecting the liquid accommodation portions is disposed to connect lower ends of the liquid accommodation portions, and accordingly, even in a case where an external force is not applied, the liquid may pass the flow path and flow into the adjacent accommodation portion due to a capillary force or the like. 
     Therefore, it is necessary to provide a test container which does not allow a flow of a liquid from an accommodation portion that holds the liquid to an adjacent accommodation portion, in a case where no external force is applied, and which has excellent liquid feeding properties, in a case of feeding the liquid by applying the external force. 
     The technology of the present disclosure is made in view of the above circumstance, and an object thereof is to provide a test container, comprising at least two accommodation portions capable of accommodating a liquid and having excellent liquid feeding properties. 
     There is provided a test container of the present disclosure comprising: at least two accommodation portions each capable of accommodating a liquid and internally provided; a flow path connecting the two accommodation portions to each other at respective upper end positions thereof and internally provided; and a flexible film deformable inwards of at least one accommodation portion at a portion forming an upper wall surface of the one accommodation portion, in which the liquid accommodated in the one accommodation portion is fed to the other accommodation portion via the flow path due to deformation of the flexible film towards the one accommodation portion, and
         a breaking elongation of the flexible film is 100% to 600%.       

     In the test container of the present disclosure,
         it is preferable that, in a case where a thickness of the flexible film is t μm, a modulus of elasticity of the flexible film is α MPa, and a depth of the one accommodation portion is d μm,   relationships of 0.03≤t/d≤2.5 and   2,000≤α×t≤250,000 are satisfied.       

     In the test container of the present disclosure,
         it is preferable that relationships of 0.03≤t/d≤1.8 and   2,000≤α×t≤110,000 are satisfied.       

     In the test container of the present disclosure,
         it is preferable that relationships of 0.08≤t/d≤1.0 and   2,000≤α×t≤50,000 are satisfied.       

     In the test container of the present disclosure,
         it is preferable that relationships of 0.2≤t/d≤0.4 and   4,000≤α×t≤20,000 are satisfied.       

     In the test container of the present disclosure, it is preferable that the breaking elongation is 200% to 500%. 
     It is preferable that the test container of the present disclosure further comprises: a container main body portion in which a portion forming each of the at least two accommodation portions and the flow path is open; and an upper lid member including the flexible film, and the at least two accommodation portions and the flow path are formed by covering the opening of the container main body portion with the upper lid member. 
     In the test container of the present disclosure, the upper lid member may have flexibility over an entire area. 
     In the test container of the present disclosure, it is preferable that the flexible film consist of any of a silicone resin, a fluororesin, polyolefin, and polycarbonate. 
     The test container of the present disclosure may further comprise a first accommodation portion; a second accommodation portion as the one accommodation portion; a third accommodation portion as the other accommodation portion; a first flow path connecting the first accommodation portion and the second accommodation portion to each other at respective upper end positions thereof; and a second flow path connecting the second accommodation portion and the third accommodation portion to each other at respective upper end positions thereof. 
     The test container of the present disclosure may further comprise a liquid return prevention structure which prevents a backflow of the liquid to the first accommodation portion, in a case where the liquid accommodated in the second accommodation portion is fed to the third accommodation portion via the second flow path due to deformation of the flexible film toward the second accommodation portion. 
     In the test container of the present disclosure, the liquid return prevention structure may have a structure in which a height from an inner bottom surface of the second accommodation portion to an inner bottom surface of the first flow path is higher than a height from the inner bottom surface of the second accommodation portion to an inner bottom surface of the second flow path. 
     In the test container of the present disclosure, the liquid return prevention structure may have a structure of the first flow path and the second flow path in which a water contact angle of an inner surface of the first flow path is set to be greater than a water contact angle of an inner surface of the second flow path. 
     In the test container of the present disclosure, the liquid return prevention structure may have a structure of a stepped portion which is provided between the first flow path and the second accommodation portion and which includes two or more steps from an inner bottom surface of the second accommodation portion. 
     The test container of the present disclosure may further include a chromatographic carrier for performing a nucleic acid test, and a carrier accommodation portion accommodating the chromatographic carrier. 
     In the test container of the present disclosure, the first accommodation portion may accommodate a first liquid containing magnetic particles, the second accommodation portion may accommodate separated magnetic particles separated from the first liquid, and the first flow path may allow the separated magnetic particles to pass. 
     According to the technology of the present disclosure, it is possible to obtain excellent liquid feeding properties in a test container comprising at least two accommodation portions each capable of accommodating a liquid. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a perspective view showing a schematic configuration of a test container  60 . 
         FIG. 2  is a cross-sectional view showing a schematic configuration of the test container  60 . 
         FIG. 3  is a diagram showing a liquid feeding method of the test container  60 . 
         FIG. 4  is an exploded perspective view showing a schematic configuration of a test container  1 . 
         FIG. 5  is a cross-sectional view showing a schematic configuration of the test container  1 . 
         FIG. 6  is a cross-sectional view showing a schematic configuration of the test container  2 . 
         FIG. 7  is a cross-sectional view showing a schematic configuration of the test container  3 . 
         FIG. 8  is a cross-sectional view showing a schematic configuration of the test container  4 . 
         FIG. 9  is a cross-sectional view showing a schematic configuration of the test container  5 . 
         FIG. 10  is a cross-sectional view showing a schematic configuration of the test container  6 . 
         FIG. 11  is a schematic configuration diagram of a nucleic acid extraction test device  100 . 
         FIG. 12  is an exploded perspective view of a test container and a diagram showing a main part of a dispenser. 
         FIG. 13  is a diagram showing a cross-sectional view of a test container and a magnet. 
         FIG. 14  is a diagram showing a cross-sectional view of a test container and a main part of a pressing machine. 
         FIG. 15  is a plan view of a main body portion of the test container of examples and comparative examples. 
         FIG. 16  is a diagram for explaining a measuring method for evaluating liquid feeding properties. 
         FIG. 17  is a diagram showing a relationship between upper lid member thickness/accommodation portion depth, modulus of elasticity×upper lid member thickness, and liquid feeding properties. 
     
    
    
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     Hereinafter, an example of an embodiment according to the present invention will be described with reference to the drawings. A front direction, a rear direction, an upward direction, a downward direction, a left direction, and a right direction used in the description below correspond to “FR”, “RR”, “UP”, “DO”, “LH”, and “RH”, respectively, in the each drawing. Since these directions are defined for convenience of description, a device configuration is not limited to these directions. The FR side is an upstream side and the RR side is a downstream side in the use of a container. In addition, the scales and the like of the respective constituent elements in the drawings are suitably changed from the actual scales for the sake of easy visual recognition. 
     Test Container of One Embodiment 
     A test container  60  according to one embodiment will be described.  FIG. 1  is an exploded perspective view showing a schematic configuration of the test container  1 .  FIG. 2  is a cross-sectional view showing a schematic configuration of the test container  60 . 
     The test container  60  is internally provided with at least two accommodation portions  65  and  66  each capable of accommodating a liquid and a flow path  68  connecting the two accommodation portions  65  and  66  to each other at respective upper end positions thereof, and a portion  64 A forming an upper wall surface  65   b  of the at least one accommodation portion  65  consists of a flexible film that is deformable inwards of the accommodation portion  65 . The test container  60  feeds the liquid accommodated in the one accommodation portion  65  to the other accommodation portion  66  via the flow path  68  by deforming the flexible film toward the one accommodation portion  65 . 
     Here, a breaking elongation of the flexible film is 100% to 600%. 
     In this example, the test container  60  includes a main body portion  62  and an upper lid member  64 . The main body portion  62  has an opening at a portion forming each of the two accommodation portions  65  and  66  and the flow path  68 . The test container has a configuration in which the two accommodation portions  65  and  66  and the flow path  68  are formed therein by covering the opening of the main body portion  62  with the upper lid member  64 . That is, the main body portion  62  configures inner bottom surfaces  65   a  and  66   a  and side wall surfaces of the accommodation portions  65  and  66 , and an inner bottom surface  68   a  and a side wall surface of the flow path  68 , and the upper lid member  64  configures upper wall surfaces  65   b  and  66   b  of the accommodation portions  65  and  66  and an upper wall surface  68   b  of the flow path  68 . However, the present invention is not limited to this configuration, as long as it has a configuration of including each accommodation portion and each flow path therein. 
     In this example, the upper lid member  64  has flexibility throughout. However, the entire upper lid member  64  may not have to be flexible, as long as the portion  64 A configuring at least the upper wall surface  65   b  of the at least one accommodation portion  65  of the test container  60 , that is, the portion  64 A of the upper lid member  64  has a flexible portion deformable in a direction toward the accommodation portion  65 . 
     The test container  60  includes the flow path  68  at the upper end position of the two accommodation portions  65  and  66 . Accordingly, the liquid accommodated in the accommodation portion is difficult to flow into the flow path, compared to a case where the flow path is included at a lower end or in the middle. Therefore, it is possible to prevent a passage of the liquid into the flow path due to a capillary phenomenon or the like without applying an external force. Meanwhile, since the portion  64 A deformable toward the inside of the accommodation portion  65  is included at the upper portion of the one accommodation portion  65 , the portion  64 A is deformed toward the inside of the accommodation portion  65  to reduce a volume of the accommodation portion  65 , thereby simply realizing liquid feeding to the other accommodation portion  66  by pushing the liquid accommodated in the accommodation portion  65 . 
     A method for feeding a liquid of the test container  60  will be described together with a schematic configuration of a liquid feeding device  70  including the test container  60 .  FIG. 3  is a diagram for explaining a schematic configuration of the liquid feeding device  70  and a liquid feeding method. The liquid feeding device  70  includes a test container  60  and a pressing machine  50  including a plunger  52  as a pressing portion. 
     The pressing machine  50  presses the portion  64 A forming the upper wall surface  65   b  of the one accommodation portion  65  of the test container  60  toward the inside of the accommodation portion  65  using the plunger  52 . In this example, the pressing machine  50  includes a cylinder  54  which guides the plunger  52  during the pressing operation. 
     As shown in the lower diagram of  FIG. 3 , the pressing machine  50  presses the portion  64 A of the upper lid member  64  toward the inside of the accommodation portion  65 , so that the flexible portion  64 A is deformed to the accommodation portion  65  side. Accordingly, the volume of the accommodation portion  65  can be reduced and a liquid L in the accommodation portion  65  can be fed to the other accommodation portion  66 . The pressing portion included in the pressing machine  50  is not limited to the plunger as long as it can press the portion  64 A toward the inside of the accommodation portion  65 , and a rod-shaped pressing indenter, a cylinder, or the like can be selected. In addition, as for a tip shape, it is possible to appropriately select a shape such as a cylinder, a prism, a hemisphere, a cone, a polygonal pyramid, a flat shape, or a wedge shape. 
     Since at least the portion  64 A of the test container  60  is a flexible film having a breaking elongation of 100% to 600%, the portion  64 A is pressed to extend from the outside towards the inside of the accommodation portion  65 , to be deformed towards the inside of the accommodation portion  65 , and accordingly, the liquid can be fed. In a case where the breaking elongation of the flexible film is 100% or more, the flexible film can be deformed without being broken and excellent liquid feeding can be performed. In addition, in a case where the breaking elongation of the flexible film is 600% or less, the flexible film is prevented from being bent in a case of manufacturing the test container, and a manufacturing yield is improved. 
     The breaking elongation of the flexible film is 100% to 600%, more preferably 200% to 500%, and even more preferably 200% to 400%. 
     In a case where a thickness of the flexible film is t μm, a modulus of elasticity of the flexible film is α MPa, and a depth of the one accommodation portion  65  is d μm,
         relationships of 0.03≤t/d≤2.5 and 2,000≤α×t≤250,000   are preferably satisfied,   relationships of 0.03≤t/d≤1.8 and 2,000≤α×t≤110,000 are more preferably satisfied,   relationships of 0.08≤t/d≤1.0 and 2,000≤α×t≤50,000   are even more preferably satisfied, and   relationships of 0.2≤t/d≤0.4 and 4,000≤α×t≤20,000   are particularly preferably satisfied.       

     By setting the breaking elongation of the flexible film to be 100% to 600% and satisfying the relationships of 0.03≤t/d≤2.5 and 2,000≤α×t≤250,000, deformability of the upper lid is excellent which leads easy deformation, excellent followability with respect to indentation is obtained, and the liquid feeding properties can be further improved. In addition, by satisfying the relationships of 0.03≤t/d≤1.8 and 2,000≤α×t≤110,000, further satisfying the relationships of 0.08≤t/d≤1.0 and 2,000≤α×t≤50,000, and particularly satisfying the relationships of 0.2≤t/d≤0.4 and 4,000≤α×t≤20,000, the liquid feeding properties can be further improved. 
     As a material of the flexible film, a silicone resin, a fluororesin, polyolefin, polycarbonate, and the like are suitable. 
     A dispensing port for dispensing a liquid may be provided in a portion of the upper lid member  64  that forms each of the upper wall surfaces  65   b  and  66   b  of the accommodation portions  65  and  66 . The dispensing port is opened at the time of dispensing but is preferably sealed at other times. Alternatively, the upper lid member  64  may be provided with no dispensing port, and the upper lid member  64  may be covered and adhered to an upper surface of the main body portion  62  after injecting the liquid to each of the accommodation portions  65  and  66 . 
     As the material of the main body portion  62 , any known resin-molded plastic materials can be used without particular limitation. Examples thereof include an acrylic resin such as a polymethyl methacrylate resin (PMMA), a polyolefin resin such as a polycarbonate resin, polyethylene (PE), polypropylene (PP), an ethylene-vinyl acetate copolymer (EVA), a cycloolefin resin such as a cycloolefin polymer (COP) and a cyclic olefin copolymer (COC), a silicone resin, a fluororesin, a polystyrene resin, a polyvinyl chloride resin, a phenol resin, a urethane resin, a polyester resin, an epoxy resin, and a cellulose resin. Particularly, from viewpoints of heat resistance and transparency, a polycarbonate resin, polypropylene, a cycloolefin resin, a silicone resin, and a fluororesin are preferable. In addition, a copolymer of these resins may be used. 
     A size (volume) of the accommodation portions  65  and  66  is, for example, approximately 1 μL (microliter) to several hundreds μL. 
     The test container  60  of the embodiment of the embodiment includes two accommodation portions, but the test container of the present disclosure may include three or more accommodation portions. 
     In a case where the test container includes a first accommodation portion; a second accommodation portion, a third accommodation portion, a first flow path connecting the first accommodation portion and the second accommodation portion to each other at respective upper end positions thereof, and a second flow path connecting the second accommodation portion and the third accommodation portion to each other at respective upper end positions thereof, it is more preferable to provide the test container including the liquid return prevention structure which prevents a backflow of the liquid to the first accommodation portion, in a case where the liquid accommodated in the second accommodation portion is fed to the third accommodation portion via the second flow path. The test containers  1  to  6  will be described below as an example having a liquid return prevention structure. 
     Test Container  1   
     The test container  1  will be described.  FIG. 5  is a cross-sectional view showing a schematic configuration of the test container  1 . The test container  1  shown in  FIG. 1 ,  FIG. 2 , and  FIG. 3  includes a container main body  10  being internally provided with a first accommodation portion  21 , a second accommodation portion  22 , and a third accommodation portion  23  each capable of accommodating a liquid, a first flow path  31  connecting the first accommodation portion  21  and the second accommodation portion  22  to each other at respective upper end positions thereof, and a second flow path connecting the second accommodation portion  22  and the third accommodation portion  23  to each other at respective upper end positions thereof. The second accommodation portion  22  corresponds to the one accommodation portion, and the third accommodation portion  23  corresponds to the other accommodation portion. The container main body  10  consists of a flexible film deformable inwards of the second accommodation portion  22  on at least a portion  14 A forming an upper wall surface  22   b  of the second accommodation portion  22 . In the test container  1 , the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the flow path  32  due to deformation of the flexible film towards the second accommodation portion  22 . Here, a breaking elongation of the flexible film is 100% to 600%. 
     In this example, the container main body  10  includes a main body portion  12  and an upper lid member  14 . The main body portion  12  has an opening in a portion forming each of the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23 . The container main body  10  has a configuration in which the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23  are formed therein by covering the opening of the main body portion  12  with the upper lid member  14 . In other words, the main body portion  12  configures the inner bottom surfaces  21   a  to  23   a  and the side wall surfaces of the accommodation portions  21  to  23 , and the inner bottom surfaces  31   a  and  32   a  and the side wall surfaces of the flow paths  31  and  32 , and the upper lid member  14  configures the upper wall surfaces  21   b  to  23   b  of the accommodation portions  21  to  23  and the upper wall surfaces  31   b  and  32   b  of the flow paths  31  and  32 . However, the present invention is not limited to this configuration, as long as it has a configuration of including each accommodation portion and each flow path therein. 
     In this example, the upper lid member  14  has flexibility throughout. However, the entire upper lid member  14  does not have to be flexible, as long as the portion  14 A configuring at least the upper wall surface  22   b  of the second accommodation portion  22  of the container main body  10 , that is, the portion  14 A of the upper lid member  14  has a flexible portion deformable in a direction toward the second accommodation portion  22 . Regarding physical properties such as the breaking elongation and the modulus of elasticity of the flexible film, the thickness, and the like, the same as those described in the above embodiment can be used, and the same effect can be obtained. 
     As a liquid return prevention structure, the test container  1  has a structure in which a height h 1  from the inner bottom surface  22   a  of the second accommodation portion  22  to the inner bottom surface  31   a  of the first flow path  31  (hereinafter, referred to as a “height h 1  of the first flow path”) is higher than a height h 2  from the inner bottom surface  22   a  of the second accommodation portion  22  to the inner bottom surface  32   a  of the second flow path  32  (hereinafter, referred to as a “height h 2  of the second flow path”). In the test container  1 , the height h 1  of the inner bottom surface  31   a  of the first flow path  31  from the inner bottom surface  22   a  of the second accommodation portion  22  is defined as a height of a corner of a level difference portion between the first flow path  31  and the second accommodation portion  22  from the inner bottom surface  22   a  of the second accommodation portion  22 . In the same manner, the height h 2  of the inner bottom surface  32   a  of the second flow path  32  from the inner bottom surface  22   a  of the second accommodation portion  22  is defined as a height of a corner of a level difference portion between the second accommodation portion  22  and the second flow path  32  from the inner bottom surface  22   a  of the second accommodation portion  22 . The liquid return prevention structure is a structure for preventing a backflow of the liquid to the first accommodation portion  21 , in a case where the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the second flow path  32  due to the deformation of the portion  14 A forming the upper wall surface  22   b  of the second accommodation portion  22  in a direction toward the second accommodation portion  22 . 
     The test container  1  includes the first flow path  31  at the upper end position of the first accommodation portion  21  and the second accommodation portion  22 , and the second flow path  32  at the upper end position of the second accommodation portion  22  and the third accommodation portion  23 , respectively. Accordingly, the liquid accommodated in the accommodation portion is difficult to flow into the flow path, compared to a case where the flow path is included at a lower end or in the middle in a depth direction. Therefore, it is possible to prevent a passage of the liquid into the flow path due to a capillary phenomenon or the like without applying an external force. Meanwhile, since the portion  14 A deformable toward the inside of the second accommodation portion  22  is included at the upper portion of the second accommodation portion  22 , the portion  14 A is deformed toward the inside of the second accommodation portion  22  to reduce a volume of the second accommodation portion  22 , thereby easily realizing liquid feeding to the third accommodation portion  23  by pushing the liquid accommodated in the second accommodation portion  22 . Here, since the portion  14 A is a flexible film having a breaking elongation of 100% to 600%, the portion  14 A is pressed to extend from the outside towards the inside of the second accommodation portion  22 , to be deformed towards the inside of the second accommodation portion  22 , and accordingly, the liquid can be fed. In a case where the breaking elongation of the flexible film is 100% or more, the flexible film can be deformed without being broken and excellent liquid feeding can be performed. In a case where the breaking elongation of the flexible film is 100% or more, the flexible film can be deformed without being broken and excellent liquid feeding can be performed. The same applies to test containers  2  to  6  below. 
     Since the height h 1  of the first flow path  31  is higher than the height h 2  of the second flow path  32 , in a case where the portion  14 A of the container main body  10  is deformed in the direction toward the second accommodation portion  22  so that the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the second flow path  32 , the liquid pushed from the second accommodation portion  22  is preferentially fed to the second flow path  32  formed at a lower position. Accordingly, the liquid return to the first flow path  31  can be suppressed, and the liquid feeding properties to the third accommodation portion  23  at a downstream side is high. According to this configuration, it is possible to suppress the liquid return to the first flow path  31  and increase the liquid feeding properties to the third accommodation portion  23  with a simple configuration of providing a difference between the heights h 1  and h 2 . 
     A difference h 1 −h 2  between the height h 1  of the first flow path  31  and the height h 2  of the second flow path  32  is preferably 20% or more, more preferably 30% or more, and particularly preferably 50% or more of the height h 2  of the second flow path  32 . As the difference h 1 −h 2  is large, the liquid feeding to the second flow path  32  is further promoted, and the liquid feeding properties to the third accommodation portion  23  can be increased. 
     In the test container  1 , a corner formed by an inner bottom surface  31   a  of the first flow path  31  and an inner side surface  22   c  of the second accommodation portion  22  in a level difference portion between the inner bottom surface  31   a  of the first flow path  31  and the second accommodation portion  22  preferably has an acute angle. By setting the corner of the level difference portion to have an acute angle, it is possible to more effectively suppress the flow of the liquid accommodated in the second accommodation portion  22  to the first flow path  31 , compared to a case where the angle is equal to or greater than 90°. Therefore, it is possible to more preferentially feed the liquid accommodated in the second accommodation portion  22  to the second flow path  32 . 
     Test Container  2   
     The test container  2  will be described.  FIG. 6  is a cross-sectional view showing a schematic configuration of the test container  2 . The test container  2  includes a container main body  10 B being internally provided with the first accommodation portion  21 , the second accommodation portion  22 , and the third accommodation portion  23  each capable of accommodating a liquid, the first flow path  31  connecting the first accommodation portion  21  and the second accommodation portion  22  to each other at respective upper end positions thereof, and the second flow path  32  connecting the second accommodation portion  22  and the third accommodation portion  23  to each other at respective upper end positions thereof. The container main body  10 B has at least the portion  14 A forming the upper wall surface  22   b  of the second accommodation portion  22  having flexibility to be deformable inwards of the second accommodation portion  22 . In the drawings, the same reference numerals are used for the same elements as those of the test container  1 . Elements having the same reference numerals as those of the test container  1  are the same as those described for the test container  1 , and specific description thereof will be omitted. The same applies to the following drawings. 
     In this example, the container main body  10 B includes the main body portion  12 B and the upper lid member  14 . The main body portion  12 B has an opening in a portion forming each of the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23 . The container main body  10 B has a configuration in which the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23  are formed therein by covering the opening of the main body portion  12 B with the upper lid member  14 . In other words, the main body portion  12 B configures the inner bottom surfaces  21   a  to  23   a  and the side wall surfaces of the accommodation portions  21  to  23 , and the inner bottom surfaces  31   a  and  32   a  and the side wall surfaces of the flow paths  31  and  32 , and the upper lid member  14  configures the upper wall surfaces  21   b  to  23   b  of the accommodation portions  21  to  23  and the upper wall surfaces  31   b  and  32   b  of the flow paths  31  and  32 . However, the present invention is not limited to this configuration, as long as it has a configuration of including each accommodation portion and each flow path therein. 
     The test container  2  has a structure of the first flow path  31  and the second flow path  32  in which a water contact angle R 1  of the inner surface of the first flow path  31  is set to be greater than a water contact angle R 2  of the inner surface of the second flow path  32 , as the liquid return prevention structure. In this example, a hydrophobic surface  34  obtained by performing a hydrophobic treatment is formed on the inner surface of the first flow path  31 . 
     In order to generate a difference in a water contact angle between the inner surface of the first flow path  31  and the inner surface of the second flow path  32 , the hydrophobic treatment may be performed on the inner surface of the first flow path  31  as in this example and/or a hydrophilic treatment may be performed on the inner surface of the second flow path  32 . 
     In the test container  2 , the portion  14 A of the container main body  10 B is deformed in the direction toward the second accommodation portion  22 , so that the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the second flow path  32 . In this case, since the water contact angle of the inner surface of the first flow path  31  is greater than the water contact angle of the inner surface of the second flow path  32 , the liquid pushed from the second accommodation portion  22  is preferentially fed to the second flow path  32  having a smaller water contact angle. Accordingly, the liquid return to the first flow path  31  can be suppressed, and the liquid feeding properties to the third accommodation portion  23  at a downstream side is high. According to this configuration, it is possible to suppress the liquid return to the first flow path  31  and increase the liquid feeding properties to the third accommodation portion  23  with a simple process of only the surface treatment. 
     The surface treatment such as the hydrophilic treatment or the hydrophobic treatment is preferably formed on the entire inner surface of each flow path, but a part of the inner surface may not be treated. 
     Examples of the hydrophilic treatment include a surface modification treatment such as a corona treatment, a plasma treatment, an ozone treatment, a treatment of applying a hydrophilic coating agent, and bonding of a hydrophilic film. Examples of the hydrophobic treatment include a treatment of applying a hydrophobic coating agent such as a fluororesin or a hydrophobic silica-containing resin, a silane coupling treatment, and bonding of a water-repellent film. 
     A difference R 1 −R 2  between the water contact angle R 1  of the first flow path  31  and the water contact angle R 2  of the second flow path  32  is preferably 10° or more, more preferably 20° or more, even more preferably 40° or more, and further preferably 60° or more. 
     In the present specification, the water contact angle is a contact angle of pure water. Specifically, 1 μL of pure water is added dropwise to the inner surface of the flow path and the accommodation portion under the condition of an atmosphere temperature of 25° C., the contact angle is measured by the θ/2 method using a fully-automatic contact angle meter (model number: DM-701, Kyowa Interface Science Co., Ltd.), and an arithmetic mean value of values obtained by measuring 5 times is used. 
     Test Container  3   
     The test container  3  will be described.  FIG. 7  is a cross-sectional view showing a schematic configuration of the test container  3 . The test container  3  includes the container main body  10 C being internally provided with the first accommodation portion  21 , the second accommodation portion  22 , and the third accommodation portion  23  each capable of accommodating a liquid, the first flow path  31  connecting the first accommodation portion  21  and the second accommodation portion  22  to each other at respective upper end positions thereof, and the second flow path  32  connecting the second accommodation portion  22  and the third accommodation portion  23  to each other at respective upper end positions thereof. The container main body  10 C has at least the portion  14 A forming the upper wall surface  22   b  of the second accommodation portion  22  having flexibility to be deformable inwards of the second accommodation portion  22 . 
     In this example, the container main body  10 C includes the main body portion  12 C and the upper lid member  14 . The main body portion  12 C has an opening in a portion forming each of the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23 . The container main body  10 C has a configuration in which the first accommodation portion  21 , the first flow path  31 , the second accommodation portion  22 , the second flow path  32 , and the third accommodation portion  23  are formed therein by covering the opening of the main body portion  12 C with the upper lid member  14 . That is, the main body portion  12 C constitutes the inner bottom surfaces  21   a  to  23   a  and the side wall surfaces of the accommodation portions  21  to  23 , and the inner bottom surfaces  31   a  and  32   a  and the side wall surfaces of the flow paths  31  and  32 , respectively. The upper lid member  14  configures the upper wall surfaces  21   b  to  23   b  of the accommodation portions  21  to  23  and the upper wall surfaces  31   b  and  32   b  of the flow paths  31  and  32 . However, the present invention is not limited to this configuration, as long as it has a configuration of including each accommodation portion and each flow path therein. 
     The test container  3  has a structure of a stepped portion  40  which is provided on the second accommodation portion  22  side of the first flow path  31  and which includes two or more steps  41  and  42  from the inner bottom surface  22   a  of the second accommodation portion  22 , as the liquid return prevention structure. On the other hand, the second flow path  32  does not include a stepped portion. In addition, in this example, the stepped portion is provided on the first accommodation portion  21  side of the first flow path  31 , but the stepped portion may not be provided on the first accommodation portion  21  side. 
     In the test container  3 , the portion  14 A of the container main body  10 C is deformed in the direction toward the second accommodation portion  22 , so that the liquid accommodated in the second accommodation portion  22  is fed to the third accommodation portion  23  via the second flow path  32 . In this case, since the first flow path  31  includes the stepped portion  40  having two or more steps, a barrier in a case where the liquid accommodated in the second accommodation portion  22  passes through the first flow path  31  has two or more steps. Accordingly, the invasion of the liquid into the first flow path  31  is suppressed, and the liquid pushed out from the second accommodation portion  22  is preferentially fed to the second flow path  32  having a smaller barrier. Therefore, the liquid return to the first flow path  31  is suppressed, and the liquid feeding properties to the third accommodation portion  23  at a downstream side is high. It is possible to obtain a high effect of preventing the liquid return to the first flow path  31  by providing the stepped portion  40  in the first flow path  31 . 
     The stepped portion  40  includes a first step  41  on the second accommodation portion  22  side and a second step  42 . The stepped portion  40  is not limited to two steps and may have three steps or four or more steps. However, from a viewpoint of avoiding complication of the structure, the stepped portion  40  preferably has two or three steps. 
     The height h 1  of the first step  41  is preferably 25% or more, more preferably 30% or more, and even more preferably 50% or more of d, where d is a height (depth) from the inner bottom surface  22   a  to the upper wall surface  22   b  of the second accommodation portion  22 . 
     A height h 12  of the second step  42  is preferably 50% or more, more preferably 60% or more, and even more preferably 80% or more of the height d of the second accommodation portion  22 . A difference between the height h 12  of the second step  42  and the height h 1  of the first step  41  is preferably 20% or more of the height h 1  of the first step  41 , from a viewpoint of preventing the liquid return. The height h 12  of the second step  42  is defined as a height from the inner bottom surface  22   a  of the second accommodation portion  22  at the corner of the level difference portion with the first step  41 . 
     In the test container  3 , a corner formed by the inner bottom surface and the inner side surface forming at least one step of the stepped portion  40  preferably has an acute angle. By setting the corner of the level difference portion to have an acute angle, it is possible to more effectively suppress the flow of the liquid accommodated in the second accommodation portion  22  to the first flow path  31 , compared to a case where the angle is equal to or greater than 90°. Therefore, it is possible to more preferentially feed the liquid accommodated in the second accommodation portion  22  to the second flow path  32 . 
     As described above, the test container  1  includes a structure in which the height h 1  of the first flow path  31  is higher than the height h 2  of the second flow path  32  (hereinafter, referred to as a liquid return prevention structure  1 ). The test container  2  includes a structure of the first flow path  31  and the second flow path  32  in which the water contact angle of the inner surface of the first flow path  31  is set to be greater than the water contact angle of the inner surface of the second flow path  32  (hereinafter, referred to as a liquid return prevention structure  2 ). The test container  3  has a structure of the stepped portion  40  including two or more steps from the inner bottom surface  22   a  of the second accommodation portion  22  configured on the second accommodation portion  22  side of the first flow path  31  (hereinafter, referred to as a liquid return prevention structure  3 ). 
     It is also preferable to comprise these liquid return prevention structures  1  to  3  in combination. For example, as shown in  FIG. 8 , a test container  4  including the liquid return prevention structure  1  and the liquid return prevention structure  2  may be used. The test container  4  includes a container main body  10 E formed of a main body portion  12 E and the upper lid member  14 . The test container  4  has a structure in which the height h 1  of the first flow path and the height h 2  of the second flow path satisfy a relationship of h 1 &gt;h 2  and includes the hydrophobic surface  34  obtained by performing a hydrophobic treatment on the inner surface of the first flow path  31 , and the water contact angle of the inner surface of the first flow path  31  is higher than the water contact angle of the inner surface of the second flow path  32 . 
     As shown in  FIG. 9 , a test container  5  including the liquid return prevention structure  2  and the liquid return prevention structure  3  may be used. The test container  5  includes a container main body  10 F forming of a main body portion  12 F and the upper lid member  14 . The test container  5  includes the hydrophobic surface  34  obtained by performing a hydrophobic treatment on the inner surface of the first flow path  31  and includes the stepped portion  40  in the first flow path  31 , and the water contact angle of the inner surface of the first flow path  31  is higher than the water contact angle of the inner surface of the second flow path  32 . 
     In addition, the test container may be a test container including the liquid return prevention structure  1  and the liquid return prevention structure  3 , or as shown in  FIG. 10 , a test container  6  including all the liquid return prevention structures  1  to  3 . The test container  6  includes a container main body  10 G formed of a main body portion  12 G and the upper lid member  14 . The test container  6  has a structure in which the height h 1  of the first flow path  31  and the height h 2  of the second flow path  32  satisfy a relationship of h 1 &gt;h 2  and includes the hydrophobic surface  34  obtained by performing a hydrophobic treatment on the inner surface of the first flow path  31 , and the water contact angle of the inner surface of the first flow path  31  is higher than the water contact angle of the inner surface of the second flow path  32 . In addition, the first flow path  31  includes the stepped portion  40 . 
     According to the test container including two or three the liquid return prevention structures  1  to  3  in combination, it is possible to obtain a higher effect of the liquid return prevention, compared to a case of including only the liquid return prevention structure  1 , only the liquid return prevention structure  2 , or only the liquid return prevention structure  3 . 
     In addition, the liquid return prevention structure is not limited to the above example, and the first flow path between the second accommodation portion and the first accommodation portion may have a structure in which the liquid accommodated in the second accommodation portion relatively hardly flows, compared to the second flow path between the second accommodation portion and the third accommodation portion. For example, a structure including a valve may be included in each of the first flow path and the second flow path may be provided as the liquid return prevention structure. In a case where a valve is provided in each of the first flow path and the second flow path, the liquid is fed in a state where the valve of the first flow path is closed and valve of the second flow path is opened, in a case of feeding the liquid from the second accommodation portion to the third accommodation portion, it is possible to effectively prevent the liquid return to the first accommodation portion and improve the liquid feeding properties to the third accommodation portion. 
     Application Example to Nucleic Acid Extraction Test 
     The test container according to the embodiment of the technology of the present disclosure can be applied as, for example, a test cartridge for a nucleic acid extraction test. A nucleic acid extraction test using a test container  101  according to another embodiment of the technology of the present disclosure will be described. 
       FIG. 11  is a configuration diagram showing a schematic configuration of a nucleic acid extraction test device  100  including the test container  101 . The nucleic acid extraction test device  100  includes the test container  101 , the pressing machine  50 , a dispenser  106 , a magnetic field generation and movement unit  107 , and a transfer portion  102  for the test container  101 . 
       FIG. 12  is an exploded perspective view of the test container  101  and a diagram showing a main part of the dispenser  106 .  FIG. 13  is a diagram showing the test container  101  and a magnet M of the magnetic field generation and movement unit  107 .  FIG. 14  is a diagram showing the test container  101  and a main part of the pressing machine  50 .  FIGS. 13 and 14  show cross-sectional views taken along a line  18 - 18  of the test container  101  shown in  FIG. 12 . 
     The test container  101  includes a container main body  110  being internally provided with four accommodation portions  120  to  123  capable of accommodating a liquid, respectively, a chromatographic carrier accommodation portion  125  accommodating a chromatographic carrier  128 , and four flow paths  130 ,  131 ,  132 , and  135  therein. 
     The container main body  110  includes a main body portion  112  and an upper lid member  114 . The main body portion  112  has an opening in a portion forming each of the accommodation portions  120  to  123  and  125  and the flow paths  130 ,  131 ,  132 , and  135 . The container main body  110  has a configuration in which the accommodation portions  120  to  123  and  125  and the flow paths  130 ,  131 ,  132 , and  135  are formed therein by covering the main body portion  112  with the upper lid member  114 . The main body portion  112  configures the side wall surface and the bottom surface of each of the accommodation portions and the flow paths, and the upper lid member  114  configures the upper wall surface of each of the accommodation portions and the flow paths. In this example, the upper lid member  114  is formed of a flexible film. The upper lid member  114  is provided with an injection port (not shown) for injecting the liquid accommodated in each of the accommodation portions  120  to  123 . The tips of syringes  160  to  163  are inserted into the injection ports, respectively, and various liquids can be injected into the corresponding accommodation portions  120  to  123 . 
     The accommodation portion  120  is a magnetic particle collecting chamber (hereinafter, referred to as the magnetism collecting chamber  120 ) which accommodates a specimen solution  150  containing magnetic particles P to which a nucleic acid is adsorbed. The accommodation portion  121  is a cleaning chamber (hereinafter, referred to as a cleaning chamber  121 ) which accommodates a cleaning solution  151  and cleans a substance non-specifically adsorbed to the magnetic particles P. The accommodation portion  122  is a PCR chamber (hereinafter, referred to as a PCR chamber  122 ) which accommodates a polymerase chain reaction (PCR) solution  152 . The accommodation portion  123  is a detection chamber (hereinafter, referred to as a detection chamber  123 ) for mixing an amplified nucleic acid and a development solution  153 . 
     The flow path  130  connects a magnetism collecting chamber  120  and the cleaning chamber  121  to each other at respective upper end positions thereof. The flow path  130  includes a stepped portion on the sides of the magnetism collecting chamber  120  and the cleaning chamber  121 , to suppress the flow of the specimen solution  150  accommodated in the magnetism collecting chamber  120  to the flow path  130  and to prevent the mixing of the specimen solution  150  with the cleaning solution  151  accommodated in the cleaning chamber  121 . 
     The flow path  131  connects the cleaning chamber  121  and the PCR chamber  122  to each other at respective upper end positions thereof and the flow path  132  connects the PCR chamber  122  and the detection chamber  123  to each other at respective upper end positions thereof. The cleaning chamber  121 , the PCR chamber  122 , the detection chamber  123 , and the flow paths  131  and  132  correspond to the first accommodation portion, the second accommodation portion, the third accommodation portion, the first flow path, and the second flow path in the technology of the present disclosure, respectively. In addition, here, the liquid return prevention structure of suppressing the backflow of the liquid to the cleaning chamber  121 , in a case of feeding the liquid accommodated in the PCR chamber  122  to the detection chamber  123  through the flow path  132  may be included. In this example, the liquid return prevention structure  3  is included as the liquid return prevention structure. That is, as the liquid return prevention structure, a structure of a stepped portion including two or more steps from an inner bottom surface  122   a  of the PCR chamber  122 , which is formed on the PCR chamber  122  side of the flow path  131 , is included. 
     The liquid return prevention structure may include a structure (liquid return prevention structure  1 ) in which a height of the first flow path (flow path  131 ) is higher than a height of the second flow path (flow path  132 ). In addition, a structure of the first flow path and the second flow path in which the water contact angle of the inner surface of the first flow path is set to be greater than the water contact angle of the inner surface of the second flow path (liquid return prevention structure  2 ) may be included. Alternatively, two or more of other liquid return prevention structures and liquid return prevention structures  1  to  3  may be provided in combination. 
     The flow path  132  connects the PCR chamber  122  and the detection chamber  123  to each other at respective upper end positions thereof. The flow path  132  may include a valve (not shown), in order to prevent evaporation of the liquid in a case of adjusting a temperature of the PCR chamber. The valve may be any valve that can be opened in a case where liquid is fed from the PCR chamber  122  to the detection chamber  123 . 
     The flow path  135  connects the detection chamber  123  and the chromatographic carrier accommodation portion  125  to each other at a lower end position. 
     The magnetic particles P are particles that are attracted by magnetic force. The magnetic particles P are, for example, magnetic particles processed so as to adsorb a specific sample such as DNA. Specifically, as the magnetic particles P, model number: Magnosphere MX100/Carboxyl and model number: Magnosphere MS160/Tosyl manufactured by JSR Corporation, sicastar manufactured by Corefront, Magrapid manufactured by Sanyo Chemical Industries, Ltd. can be used. 
     As the magnetic particles P, magnetic particles having a particle size in a range of 0.01 μm to 100 μm are used. As the magnetic particles P, magnetic particles having a particle size of approximately 1 μm to 10 μm are preferably used. The magnetic particles P may be included in the magnetism collecting chamber  120  in advance, or may be injected into the magnetism collecting chamber  120  together with the specimen solution  150 . 
     The specimen solution  150  is, for example, a specimen solution containing a nucleic acid extracted from a specimen. The specimen solution  150  may include a surfactant for extracting a nucleic acid such as deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from the specimen and adsorbing the nucleic acid on the surfaces of the magnetic particles P. In addition, as the surfactant, for example, sodium dodecyl sulfate, polyoxyethylene sorbitan monolaurate (Tween 20), Triton X-100, or the like can be used. These surfactants may be used alone or in combination of a plurality thereof. A chaotropic substance such as guanidine hydrochloride may be included in order to promote extraction of nucleic acid from the specimen and surface adsorption to the magnetic particles P. In addition, instead of containing the surfactant, a nucleic acid extracted from a specimen using a column may be contained. In addition, a surfactant for suppressing aggregation of the magnetic particles P may be included. 
     The cleaning solution  151  removes the substance non-specifically adsorbed to the magnetic particles P. As the cleaning solution  151 , water or a buffer solution, an organic solvent such as ethanol and isopropyl alcohol, or the like can be used. In a case where the buffer solution is used as the cleaning solution, salt is not particularly limited, but salt of tris or phosphoric acid is preferably used. In addition, in order to suppress the elution of RNA in the cleaning step, the surfactant such as sodium dodecyl sulfate, Triton X-100, or the like may be contained. 
     The PCR solution  152  is a solution for performing a process for amplifying nucleic acid by PCR. The PCR solution  152  contains, for example, reverse transcriptase, dNTP in which four kinds of deoxyribonucleotide triphosphates are mixed, and a primer for reverse transcriptase. Transcriptase is an enzyme that synthesizes complementary deoxyribonucleic acid (cDNA) using a base sequence of RNA as a template. 
     The chromatographic carrier accommodation portion  125  accommodates the chromatographic carrier  128 . In the chromatographic carrier accommodation portion  125 , the development solution  153  containing the amplified nucleic acid is developed. The chromatographic carrier  128  is a nucleic acid chromatographic carrier and indicates whether or not the target nucleic acid is present in the development solution  153 . 
     The dispenser  106  includes the syringes  160  to  163  for adding various liquids  150  to  153  to the respective accommodation portions  120  to  123  of the test container  101 . 
     The pressing machine  50  includes a plunger  52  is configured to be able to press a region corresponding to the PCR chamber  122  of the container main body  110  (here, the upper lid member  114 ) by the plunger  52 . 
     The magnetic field generation and movement unit  107  includes the magnet M and a movement mechanism  170  that moves the magnet M. 
     The magnet M is, for example, a permanent magnet, but may be an electromagnet. As shown in  FIG. 13 , the magnet M is freely moved between positions A 0  to A 5  of the test container  101  on the upper lid member  114 . The positions A 0 , A 3 , and A 5  are positions where a magnetic force does not act on the magnetic particles P accommodated in the test container  101 , even in a case where the magnet M is disposed. The position A 1  is a position on the magnetism collecting chamber  120  and is a position where a magnetic force acts on the magnetic particles P in the magnetism collecting chamber  120  in a case where the magnet M is disposed. The position A 2  is a position on the cleaning chamber  121  and is a position where magnetic force acts on the magnetic particles P in the cleaning chamber  121  in a case where the magnet M is disposed. The position A 4  is a position on the PCR chamber  122  and is a position where a magnetic force acts on the magnetic particles P in the PCR chamber  122  in a case where the magnet M is disposed. 
     In a case of moving the magnetic particles P from the magnetism collecting chamber  120  to the cleaning chamber  121 , first, the magnet M is disposed at the position A 1 . In a case where the magnet M is disposed at the position A 1 , the magnetic particles P accommodated in the magnetism collecting chamber  120  are collected by the magnetic force of the magnet M and are attracted and collected at the position corresponding to the magnet M with the upper lid member  14  interposed therebetween. In a case where the magnet M is moved to the position A 2  along the upper lid member  14  from this state, the magnetic particles P are separated from the specimen solution  150  and moved to the cleaning chamber  121  according to the movement of the magnet M. Then, in a case where the magnet M is moved to the position A 3 , the magnetic particles P are dispersed in the cleaning solution  151 . 
     In the same manner, in a case of moving the magnetic particles P from the cleaning chamber  121  to the PCR chamber  122 , first, the magnet M is disposed at the position A 2 . In a case where the magnet M is disposed at the position A 2 , the magnetic particles P accommodated in the cleaning chamber  121  are attracted and collected at the position corresponding to the magnet M with the upper lid member  14  interposed therebetween. In a case where the magnet M is moved to the position A 4  along the upper lid member  14  from this state, the magnetic particles P are separated from the cleaning solution  151  and moved to the PCR chamber  122  along the movement of the magnet M. After that, in a case where the magnet M is moved to the position A 5 , the magnetic particles P are dispersed in the PCR solution  152 . 
     The movement mechanism  170  has a function of allowing the magnet M to pass the upper portion of the flow path  130  from the position A 1  on the magnetism collecting chamber  120 , to pass the upper portion of the flow path  131  from the position A 2  on the cleaning chamber  121 , and to freely move to the position A 4  on the PCR chamber  122 . In addition, the movement mechanism  170  moves the magnet M to the positions A 0 , A 3  and A 5  where the magnetic force does not reach the inside of the chambers  120 ,  121  and  122 . 
     The nucleic acid extraction test device  100  further includes a temperature control unit  108  (see  FIG. 13 ). The temperature control unit  108  controls a temperature of the PCR solution in the PCR chamber  122 . The temperature control unit  108  includes a heating unit such as a heater or a Peltier element for heating a solution, and a cooling unit such as a Peltier element, a fan, a heat sink, or a liquid cooling mechanism for cooling a solution. The temperature control unit  108  raises or lowers the temperature of the solution so that the temperature is adjusted to a suitable temperature in each step of a heat denaturation step, an annealing step, and an extension step in PCR. 
     A transportation unit  102  is a device that relatively moves the test container  101  relatively to the dispenser  106 , the magnetic field generation and movement unit  107 , and the pressing machine  50 . The transportation unit  102  may transport only the test container  101 , or move the respective positions of the dispenser  106 , the magnetic field generation and movement unit  107 , and the pressing machine  50  with respect to the test container  101 . 
     Nucleic Acid Extraction Test Method 
     The steps of the nucleic acid extraction test in the nucleic acid extraction test device  100  including the test container  101  will be described. 
     Pretreatment (Adsorption Process) 
     A sample containing RNA is mixed with a solution containing a surfactant that dissolves a cell membrane and the magnetic particles P to adsorb the RNA to the magnetic particles P. The sample containing RNA is not particularly limited, as long as it contains the RNA such as a biological sample and virus. As necessary, impurities may be removed with a filter or the like. 
     Magnetization Collection Process 
     The specimen solution  150  containing the magnetic particles P having RNA adsorbed, which was obtained in the pretreatment, is injected into the magnetism collecting chamber  120  by the syringe  160 . After that, the magnet M is set at the position A 1  on the magnetism collecting chamber  120 . Accordingly, the magnetic particles P accommodated in the magnetism collecting chamber  120  are attracted to the magnet M and are collected at a position corresponding to the magnet M on the upper surface to be in an aggregated state (see  FIG. 13 ). 
     In the magnetism collecting chamber  120 , the adsorption process and the magnetism collection process may be performed in time series. 
     Then, by moving the magnet M along the flow path  130 , the magnetic particles P are separated from the specimen solution  150  and moved to the cleaning chamber  121 . 
     Cleaning Step 
     In the cleaning chamber  121 , the magnetic particles P adsorbed with RNA are cleaned with the cleaning solution  151  accommodated in the cleaning chamber  121 . The cleaning chamber  121  may be filled with the cleaning solution  151  in advance, or the cleaning solution  151  may be injected after the magnetic particles P are moved. The magnet M is moved to the position (position A 3 ) where the magnetic force does not affect the cleaning chamber  121  and the magnetic particles P are dispersed in the cleaning solution  151 , thereby promoting the cleaning. By performing the cleaning, the substances other than RNA that are non-specifically bound to the magnetic particles P are removed. 
     Then, by returning the magnet M to the position A 2  on the cleaning chamber  121 , the magnetic particles P are collected again at the position corresponding to the magnet M on the upper surface, and the magnet M is moved to the position A 4  on the PCR chamber  122  along the flow path  131 , thereby separating the magnetic particles P from the cleaning solution  151  and moving the magnetic particles to the PCR chamber  122 . After that, the magnet M is moved to the position A 5  where the magnetic force does not affect the PCR chamber  122 , so that the magnetic particles P are dispersed in the PCR solution  152 . 
     PCR Process 
     In the PCR chamber  122 , the RNA adsorbed to the magnetic particles P is eluted into the PCR solution  152 , and the DNA amplification by PCR is performed. The cDNA is synthesized from the extracted RNA and the cDNA is amplified by PCR. In this case, the magnetic particles P sink to the inner bottom surface of the PCR chamber  122  due to gravity. 
     Liquid Feeding Process 
     After the PCR step, the solution containing the amplified cDNA in the PCR chamber  122  is fed to the detection chamber  123 . The test container  101  includes the flow path  131  at the upper end position of the cleaning chamber  121  and the PCR chamber  122 , and the flow path  132  at the upper end position of the PCR chamber  122  and the detection chamber  123 , respectively. Accordingly, it is possible to prevent the passage of the solution  152  from the PCR chamber  122  to the flow paths  131  and  132  due to a capillary phenomenon or the like, before this liquid feeding process. 
     As shown in  FIG. 14 , in a case where the liquid is fed, the plunger  52  is positioned on the PCR chamber  122  and the plunger  52  is pushed down along the cylinder  54 . A portion  114 A of the flexible upper lid member  114  is pushed by the plunger  52  and pushed inwards of the PCR chamber  122 . This reduces the volume of the PCR chamber  122 , so that the liquid in the PCR chamber  122  is fed to the detection chamber  123  through the flow path. In this example, the flexible film is used as the upper lid member  114 , and the flexible film has a breaking elongation of 100% to 600%. Accordingly, in a case where the portion  114 A is pressed by the plunger  52 , the flexible film can be deformed without being broken and excellent liquid feeding can be performed. In addition, since the return prevention structure is provided, most of the solution  152  in the PCR chamber  122  does not flow backward to the cleaning chamber  121  side, and a large amount of the solution extruded from the PCR chamber  122  can be fed to the detection chamber  123 . In addition, since the flow path  132  is included at the upper end position of the PCR chamber  122 , a supernatant portion of the PCR solution can be preferentially fed while the magnetic particles P are submerged on the inner bottom surface, and the magnetic particles P can be suppressed from flowing out to the detection chamber  123  side. By suppressing the magnetic particles P from flowing to the detection chamber  123 , it is possible to perform a test with less noise in the next step. 
     Detection Process 
     In the detection chamber  123 , the solution containing cDNA is mixed with the development solution. After that, the mixed liquid passes through the flow path  135  and is developed by the nucleic acid chromatographic carrier (chromatographic carrier  128 ) disposed in the chromatographic carrier accommodation portion  125 . In a case where the RNA to be tested is contained, a positive result is obtained, and in a case where not, a negative result is obtained. 
     The nucleic acid extraction test is performed as described above. 
     Hereinabove, the case where the reverse transcription PCR method is used as the amplification method has been described, but the amplification method is not limited to the reverse transcription PCR method, and well-known amplification methods such as the transcription PCR method, the isothermal amplification method (for example, Nucleic Acid Sequence-Based Amplification (NASBA), Loop-mediated Isothermal Amplification (LAMP), transcription-reverse transcription concerted (TRC), and the like) can be used. In addition, hereinabove, the case where the nucleic acid chromatography method is used as the detection method has been described above, but the detection method is not limited to the nucleic acid chromatography method, and well-known methods such as a fluorescence detection method (intercalator method, probe method, or the like), a light scattering method using gold nanoparticles, a sequence method, an electrochemical method, a piezoelectric method, and detection of a weight or a mechanical change can be used. In these cases, the container does not necessarily comprise the chromatographic carrier  128  and the accommodation portion  125  thereof. On the other hand, the test device may comprise a detection unit suitable for various detection methods of a fluorescence detection unit and the like for detecting fluorescence from the detection chamber  123 . However, the nucleic acid chromatography method is preferable because a high-priced detection system and detection equipment are not necessary and the operation in the analysis is simple. 
     By using the test container  101 , the solution containing the DNA amplified in the PCR chamber  122  can be efficiently fed to the detection chamber  123  while suppressing the backflow to the cleaning chamber  121 , and a sufficient amount of solution to be fed can be realized. Since the backflow can be suppressed to increase the amount of liquid to be fed to the detection chamber  123 , a total amount of DNA that flows into the detection chamber  123  can be increased, which leads to improvement in determination accuracy. 
     In regard to the test container  101 , a set of the test container  101 , the magnetic particles P, and various treatment liquids such as the cleaning solution  151 , the PCR solution  152 , and the development solution  153  can also be provided as a test kit. The test kit may further include other treatment liquid such as a nucleic acid eluate. In addition, as the test kit, it is also possible to provide a set of only the test container  101  and the magnetic particles P. The magnetic particles P may be set in the magnetism collecting chamber  120  of the test container  101  in advance, or may be separately prepared. 
     The technology of the present disclosure is not limited to the embodiment described above, and various modifications, changes, and improvements can be made without departing from the spirit of the invention. For example, the modification examples described above may be appropriately configured in combination. 
     EXAMPLES 
     Hereinafter, more specific examples and comparative examples of the technology of the present disclosure will be described. 
     Examples and comparative examples of containers including two accommodation portions and flow paths connecting those to each other were prepared and evaluated.  FIG. 15  is a plan view showing a main body portion  202  of the test container of examples and comparative examples.  FIG. 16  is a diagram for explaining a measuring method for evaluating liquid feeding properties. The test container  201  of each example includes two accommodation portions  205  and  206 , and a flow path  208  connecting the two accommodation portions  205  and  206  at the upper end. The two accommodation portions  205  and  206  have the same shape, and have a length L of 7.5 mm, a width W of 7.5 mm, and a depth d of 1 mm. A width of the flow path  208  is set as 1 mm and a height thereof is set as 0.2 mm (that is, an inner bottom surface of the flow path  208  is connected at a height of 0.8 mm from an inner bottom surface of the accommodation portion  205 ). 
     The test container  201  is configured with the main body portion  202  and an upper lid member  204 , and the main body portion  202  is configured with a main body portion  202 A forming side wall surfaces of the accommodation portions  205  and  206  and a side wall surface and an inner bottom surface of the flow path  208 , and a bottom surface member  202 B forming the inner bottom surfaces of the accommodation portions  205  and  206 . 
     Polycarbonate (PC) was used as the material of the main body portion  202 . Specifically, the main body portion  202 A was injection-molded using IUPILON EB-3001R manufactured by Mitsubishi Engineering Plastics Co., Ltd. As the bottom surface member  202 B, Technoloy C000 (thickness of 100 μm) manufactured by Sumika Acrylic Sales Co., Ltd. was used. The bottom surface member  202 B was roller-bonded to the bottom surface of the main body portion  202 A using an adhesive #9969 manufactured by  3 M Japan Co., Ltd. to be attached to the main body portion  202 . The upper lid member  204  was roller-bonded to the upper surface of the main body portion  202 A using a silicone adhesive NSD-50 manufactured by Nipper Co., Ltd. to obtain a test container. Here, as shown in  FIG. 16 , the upper lid member  204  was attached to the main body portion  202 A so that the one accommodation portion and the flow path are covered and the other accommodation portion is opened, for evaluation of the liquid feeding test from the one accommodation portion to the other accommodation portion. 
     A material and a thickness of the upper lid member  204  were set as follows for each example and comparative example. 
     Example 1 
     In the test container of Example 1, a polycarbonate material, Sumika Acrylic Sales Co., Ltd.: Technology (thickness of 100 μm) was used as the upper lid member  204 . 
     Example 2 
     In the test container of Example 2, a polyolefin-based material, Toray Industries, Inc.: Trefan BO (thickness of 60 μm) was used as the upper lid member  204 . 
     Example 3 
     In the test container of Example 3, a polyolefin-based material, SunTox Co., Ltd.: SunTox-CP YJ02 (thickness of 30 μm) was used as the upper lid member  204 . 
     Example 4 
     In the test container of Example 4, a film having a thickness of 90 μm manufactured by adhering three sheets of a polyolefin-based material, SunTox Co., Ltd.: SunTox-CP YJ02 (thickness of 30 μm) to a vacuum laminator was used as the upper lid member  204 . 
     Example 5 
     In the test container of Example 5, a polyester-based material, Toyobo Co., Ltd.: Cosmoshine A4300 (thickness of 50 μm) was used as the upper lid member  204 . 
     Example 6 
     In the test container of Example 6, a fluorine-based resin material, Daikin Industries, Ltd.: NEOFLON PFA AF-0100 (thickness of 100 μm) was used as the upper lid member  204 . 
     Example 7 
     In the test container of Example 7, a fluorine-based resin material, Daikin Industries, Ltd.: NEOFLON ETFE EF-0100 (thickness of 100 μm) was used as the upper lid member  204 . 
     Example 8 
     In the test container of Example 8, silicone, Tomita Mateqs Co., Ltd.: GFSC6000 (thickness of 100 μm) was used as the upper lid member  204 . 
     Example 9 
     In the test container of Example 9, silicone, Tomita Mateqs Co., Ltd.: GFSC6000 (thickness of 200 μm) was used as the upper lid member  204 . 
     Example 10 
     In the test container of Example 10, silicone, Tomita Mateqs Co., Ltd.: GFSC6000 (thickness of 300 μm) was used as the upper lid member  204 . 
     Example 11 
     In the test container of Example 11, silicone, Tomita Mateqs Co., Ltd.: GFSC6000 (thickness of 1,000 μm) was used as the upper lid member  204 . 
     Example 12 
     In the test container of Example 12, a polyolefin-based material, UNITIKA Ltd., cast film of Arrowbase SE1013N (thickness of 200 μm) was used as the upper lid member  204 . 
     The cast film was manufactured by the following procedure. 
     Manufacturing of Cast Film 
     A resin composition 1 obtained by mixing the following components was poured into a PFA Petri dish manufactured by Tokyo Materials Co., Ltd. so that a dry film thickness was 200 μm, dried at 30° C. for 10 days, and then heated at 100° C. for 10 minutes. Then, the dry film was peeled from the Petri dish to obtain a cast film. 
     The resin composition 1 in the manufacturing of the cast film was obtained by mixing the following components. 
     Resin Composition 1 
     As the resin composition 1, a mixture of the following components was used.
         Arrowbase SE1013N (UNITIKA Ltd.): 98.00 parts by mass
           Fluorine-based surfactant (sodium=bis(3,3,4,4,5,5,6,6,6-nonafluorohexyl)=2-sulfonite oxysuccinate, manufactured by FUJIFILM Fine Chemicals Co., Ltd., 2% water dilution): 2.00 parts by mass   
               

     Example 13 
     In the test container of Example 13, a polyolefin-based material, UNITIKA Ltd., cast film of Arrowbase SE1013N (thickness of 400 μm) was used as the upper lid member  204 . The cast film was manufactured by the following procedure. 
     Manufacturing of Cast Film 
     The resin composition 1 obtained in Example 12 was poured into a PFA Petri dish manufactured by Tokyo Materials Co., Ltd. so that a dry film thickness was 400 μm, dried at 30° C. for 10 days, and then heated at 100° C. for 10 minutes. Then, the dry film was peeled from the Petri dish to obtain a cast film. 
     Example 14 
     In the test container of Example 14, a polyolefin-based material, Toho Chemical Industry Co., Ltd, case film of Hitech S3121 (thickness of 600 μm) was used as the upper lid member. 
     The cast film was manufactured by the following procedure. 
     Manufacturing of Cast Film 
     A resin composition 2 obtained by mixing the following components was poured into a PFA Petri dish manufactured by Tokyo Materials Co., Ltd. so that a dry film thickness was 600 μm, dried at 30° C. for 10 days, and then heated at 100° C. for 10 minutes. Then, the dry film was peeled from the Petri dish to obtain a cast film. 
     The resin composition 2 in the manufacturing of the cast film was obtained by mixing the following components. 
     Resin Composition 2 
     As the resin composition 2, a mixture of the following components was used.
         High Tech S3121 (Toho Chemical Industry Co., Ltd.): 97.53 parts by mass
           Fluorine-based surfactant (sodium=bis(3,3,4,4,5,5,6,6,6-nonafluorohexyl)=2-sulfonite oxysuccinate, manufactured by FUJIFILM Fine Chemicals Co., Ltd., 2% water dilution): 2.47 parts by mass   
               

     Example 15 
     In the test container of Example 15, a polyolefin-based material, Toho Chemical Industry Co., Ltd, case film of Hitech S3121 (thickness of 800 μm) was used as the upper lid member. 
     The cast film was manufactured in the same manner as in Example 14. That is, a cast film having a dry film thickness of 800 μm was manufactured using the resin composition 2. 
     Example 16 
     In the test container of Example 16, a polyolefin-based material, UNITIKA Ltd., cast film of Arrowbase DA1010 (thickness of 500 μm) was used as the upper lid member. 
     The cast film was manufactured by the following procedure. 
     Manufacturing of Cast Film 
     A resin composition 3 obtained by mixing the following components was poured into a PFA Petri dish manufactured by Tokyo Materials Co., Ltd. so that a dry film thickness was 500 μm, dried at 30° C. for 10 days, and then heated at 100° C. for 10 minutes. Then, the dry film was peeled from the Petri dish to obtain a cast film. 
     The resin composition 3 in the manufacturing of the cast film was obtained by mixing the following components. 
     Resin Composition 3 
     As the resin composition 3, a mixture of the following components was used.
         Arrowbase DA1010 (UNITIKA Ltd.): 97.56 parts by mass
           Fluorine-based surfactant (sodium=bis(3,3,4,4,5,5,6,6,6-nonafluorohexyl)=2-sulfonite oxysuccinate, manufactured by FUJIFILM Fine Chemicals Co., Ltd., 2% water dilution): 2.44 parts by mass   
               

     Example 17 
     In the test container of Example 17, a polyolefin-based material, Sumitomo Seika Chemicals Co., Ltd., cast film of SEPOLSION VA-407 (thickness of 1,500 μm) was used as the upper lid member. 
     The cast film was manufactured by the following procedure. 
     Manufacturing of Cast Film 
     A resin composition 4 obtained by mixing the following components was poured into a PFA Petri dish manufactured by Tokyo Materials Co., Ltd. so that a dry film thickness was 1,500 μm, dried at 30° C. for 10 days, and then heated at 100° C. for 10 minutes. Then, the dry film was peeled from the Petri dish to obtain a cast film. 
     The resin composition 4 in the manufacturing of the cast film was obtained by mixing the following components. 
     Resin Composition 4 
     As the resin composition 4, a mixture of the following components was used.
         Pure water: 37.71 parts by mass   SEPOLSION VA-407 (Sumitomo Seika Co., Ltd.): 59.70 parts by mass
           Fluorine-based surfactant (sodium=bis(3,3,4,4,5,5,6,6,6-nonafluorohexyl)=2-sulfonite oxysuccinate, manufactured by FUJIFILM Fine Chemicals Co., Ltd., 2% water dilution): 2.99 parts by mass   
               

     Example 18 
     In the test container of Example 18, a polyolefin-based material, Sumitomo Seika Chemicals Co., Ltd., cast film of SEPOLSION VA-407 (thickness of 2,000 μm) was used as the upper lid member. 
     Comparative Example 1 
     In the test container of Comparative Example 1, silicone, Sumika Acrylic Sales Co., Ltd.: Technoloy C000 (thickness of 100 μm) was used as the upper lid member  204 . 
     Comparative Example 2 
     In the test container of Comparative Example 2, silicone, Shin-Etsu Chemical Co., Ltd.: KER-4700-UV (thickness of 100 μm) was used as the upper lid member  204 . KER-4700-UV was applied to Therapy RX manufactured by Toray Co., Ltd. to have a thickness of 100 μm, and then the curing treatment was performed by irradiating with light of a metal halide lamp (MAL625NAL manufactured by GS Yuasa International Ltd.) having an exposure intensity of 300 mJ/cm 2  in a low oxygen atmosphere having an oxygen concentration of 1,000 ppm or less. Finally, the therapy was peeled off to obtain a film having a thickness of 100 μm. 
     Comparative Example 3 
     In the test container of Comparative Example 3, a polystyrene-based material, Mitsubishi Chemical Corporation: Santo Clear AP (thickness of 180 μm) was used as the upper lid member  204 . 
     With respect to the upper lid members of the containers of Examples 1 to 11 and Comparative Examples 1 to 3 obtained as described above, the modulus of elasticity and the breaking elongation were measured. 
     Modulus of Elasticity and Break Elongation 
     The upper lid member of each example was punched to have a width of 10 mm and a length of 50 mm using a punching cutter. A tensile test at a tensile rate of 50 mm/min was performed using Tensileon RTF-1310 manufactured by A&amp;D Co., Ltd. The modulus of elasticity and breaking elongation were obtained from the obtained stress-strain curve. The cast film and the non-stretched film were tested with a film punched in an arbitrary direction, and an average value measured 5 times was used. The stretched film was tested with samples punched in a machine direction (MD) and a transverse direction (TD), and an average value measured 5 times each for MD and TD was used. 
     The modulus of elasticity and breaking elongation for the upper lid member in each example are shown in Table 1. 
     The liquid feeding properties of the containers of Examples 1 to 11 and Comparative Examples 1 to 3 were evaluated by the following method. 
     Evaluation of Liquid Feeding Properties 
     After filling the one accommodation portion  205  in the test container with water, the ball plunger  52  as a pressing portion was pushed to the vicinity of the center of the portion  204 A forming the upper wall surface  205   b  of the accommodation portion  205  by 0.3 mm. Accordingly, the liquid fed to the other accommodation portion  206  was recovered and weighed. An average value measured 5 times was evaluated according to the following criteria. Practically, D or higher is required. In addition, practically, C or higher is preferable, B or higher is more preferable, and A is further preferable. 
     A: 1 mg or more 
     B: less than 1 mg and 0.75 mg or more 
     C: less than 0.75 mg and 0.5 mg or more 
     D: less than 0.5 mg and 0.25 mg or more 
     E: Less than 0.25 mg, or the film was damaged during each measurement, and the measurement could not be performed. 
     Table 1 collectively shows a structure, measurement, and evaluation results of the test container of each example. 
     
       
         
           
               
               
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                   
                 Test container 
                   
               
            
           
           
               
               
               
               
            
               
                   
                 Container  
                 Upper lid member 
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 main body 
                   
                   
                   
                 Physical properties 
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 Accom- 
                   
                   
                   
                   
                   
                   
                 Thickness 
                   
               
               
                   
                 modation 
                   
                   
                   
                 Breaking 
                 Modulus  
                 Modulus of 
                 t/accom- 
                 Evaluation 
               
               
                   
                 portion  
                   
                   
                 Thick- 
                 elon- 
                 of 
                 elasticity α × 
                 modation 
                 Liquid  
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 depth d 
                 Material 
                 ness t 
                 gation 
                 elasticity  
                 thickness t 
                 portion  
                 feeding 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 [μm] 
                 Type 
                 Product number 
                 [μm] 
                 [%] 
                 α [MPa] 
                 [MPa· μm] 
                 depth d 
                 properties 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
            
               
                 Example 1 
                 1000 
                 Polycarbonate-based 
                 Technoloy C000 
                 100 
                 105 
                 1568 
                 156800 
                 0.100 
                 D 
               
               
                 Example 2 
                 1000 
                 Polyolefin-based 
                 Trefan BO 
                 60 
                 110 
                 2032 
                 121920 
                 0.060 
                 D 
               
               
                 Example 3 
                 1000 
                 Polyolefin-based 
                 SunTox-CP YJ02 
                 30 
                 600 
                 70 
                 2100 
                 0.030 
                 C 
               
               
                 Example 4 
                 1000 
                 Polyolefin-based 
                 SunTox-CP YJ02 
                 90 
                 511 
                 68 
                 6120 
                 0.090 
                 B 
               
               
                 Example 5 
                 1000 
                 Polyester-based 
                 Cosmoshine A4300 
                 50 
                 133 
                 4316 
                 215800 
                 0.050 
                 D 
               
               
                 Example 6 
                 1000 
                 Fluorine-based 
                 NEOFLON AF-0100 
                 100 
                 467 
                 732 
                 73200 
                 0.100 
                 C 
               
               
                 Example 7 
                 1000 
                 Fluorine-based 
                 NEOFLON EF-0100 
                 100 
                 494 
                 480 
                 48000 
                 0.100 
                 B 
               
               
                 Example 8 
                 1000 
                 Silicone 
                 GFSC6000 
                 100 
                 165 
                 22 
                 2200 
                 0.100 
                 B 
               
               
                 Example 9 
                 1000 
                 Silicone 
                 GFSC6000 
                 200 
                 203 
                 20 
                 4000 
                 0.200 
                 A 
               
               
                 Example 10 
                 1000 
                 Silicone 
                 GFSC6000 
                 300 
                 262 
                 17 
                 5100 
                 0.300 
                 A 
               
               
                 Example 11 
                 1000 
                 Silicone 
                 GSSC6000 
                 1000 
                 114 
                 14 
                 14000 
                 1.000 
                 B 
               
               
                 Example 12 
                 1000 
                 Polyolefin-based 
                 Arrowbase SE1013N 
                 200 
                 331 
                 100 
                 20000 
                 0.200 
                 A 
               
               
                 Example 13 
                 1000 
                 Polyolefin-based 
                 Arrowbase SE1013N 
                 400 
                 343 
                 124 
                 49600 
                 0.400 
                 B 
               
               
                 Example 14 
                 1000 
                 Polyolefin-based 
                 Hitech S3121 
                 600 
                 322 
                 155 
                 93000 
                 0.600 
                 C 
               
               
                 Example 15 
                 1000 
                 Polyolefin-based 
                 Hitech S3121 
                 800 
                 280 
                 150 
                 120000 
                 0.800 
                 D 
               
               
                 Example 16 
                 1000 
                 Polyolefin-based 
                 Arrowbase DA1010 
                 500 
                 280 
                 364 
                 182000 
                 0.500 
                 D 
               
               
                 Example 17 
                 1000 
                 Polyolefin-based 
                 SEPOLSION VA-407 
                 1500 
                 437 
                 65 
                 97500 
                 1.500 
                 C 
               
               
                 Example 18 
                 1000 
                 Polyolefin-based 
                 SEPOLSION VA-407 
                 2000 
                 403 
                 61 
                 122000 
                 2.000 
                 D 
               
               
                 Comparative 
                 1000 
                 Acryl 
                 Technoloy S000 
                 100 
                 22 
                 1591 
                 159057 
                 0.100 
                 E 
               
               
                 Example 1 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 Comparative 
                 1000 
                 Silicone 
                 KER-4700-UV 
                 100 
                 20 
                 302 
                 30200 
                 0.100 
                 E 
               
               
                 Example 2 
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 Comparative 
                 1000 
                 Polystyrene 
                 Santo Clear SP 
                 180 
                 15 
                 2655 
                 477900 
                 0.180 
                 E 
               
               
                 Example 3 
               
               
                   
               
            
           
         
       
     
     In  FIG. 17 , the graph was plotted by setting the vertical axis as the upper lid member thickness/accommodation portion depth (t/d) and the horizontal axis as the modulus of elasticity×the thickness of the upper lid member (α×t) for the examples, and a range in which it is expected that the liquid feeding properties A to D are obtained based on the evaluation results of each example is shown. In the drawing, the data points of each example are shown with the example number. 
     As shown in Table 1, in Examples 1 to 18 in which the breaking elongation of the upper lid member was in a range of 100% to 600%, the liquid feeding properties are at a level of D or higher and excellent liquid feeding properties were obtained, compared to Comparative Examples 1 to 3 in which the breaking elongation was less than 100%. 
     In Examples 3, 4, 6 to 14, 17, the relationships of 0.03≤t/d≤1.8 and 2,000≤α×t≤110,000 were satisfied and the liquid feeding properties were higher than those in Examples 1, 2, 5, 15, 16, and 18. These were evaluated as the liquid feeding properties at the level of C or higher. 
     Among those, in Examples 4 and 7 to 13, the relationships of 0.08≤t/d≤1.0 and 2,000≤α×t≤50,000 were satisfied and the liquid feeding properties were higher than those in Examples 3, 5, 6, 14, and 17. These were evaluated as the liquid feeding properties at the level of B or higher. 
     Among those, in Examples 9, 10, and 12, the relationships of 0.2≤t/d≤0.4 and 4,000≤α×t≤20,000 were satisfied and the liquid feeding properties were highest among Examples 1 to 11. These were evaluated as the liquid feeding properties at the level of A. 
     EXPLANATION OF REFERENCES 
     
         
         
           
               1 ,  2 ,  3 ,  4 ,  5 ,  6 ,  60 : Test container 
               10 ,  10 B,  10 C,  10 E,  10 F,  10 G: Container main body 
               12 ,  12 B,  12 C,  12 E,  12 F,  12 G: Main body portion 
               14 : Upper lid member 
               14 A: Portion 
               21 : First accommodation portion 
               21   a : Inner bottom surface of first accommodation portion 
               21   b : Upper wall surface of first accommodation portion 
               22 : Second accommodation portion 
               22   a : Inner bottom surface of second accommodation portion 
               22   b : upper wall surface of second accommodation portion 
               22   c : Inner side surface of second accommodation portion 
               23 : Third accommodation portion 
               31 : First flow path 
               31   a : Inner bottom surface of first flow path 
               31   b : Upper wall surface of first flow path 
               32 : Second flow path 
               32   a : Inner wall surface of second flow path 
               32   b : Upper wall surface of first flow path 
               34 : Hydrophobic surface 
               40 : Stepped portion 
               41 ,  42 : Step 
               50 : Pressing machine 
               52 : Plunger 
               54 : Cylinder 
               60 : Test container 
               62 : Main body portion 
               64 : Upper lid member 
               64 A: Portion 
               65 : One accommodation portion 
               65   a : Inner bottom surface of one accommodation portion 
               65   b : Upper wall surface of one accommodation portion 
               66 : Other accommodation portion 
               66   a : Inner bottom surface of other accommodation portion 
               66   b : Upper wall surface of other accommodation portion 
               68 : Flow path 
               68   a : Inner bottom surface of flow path 
               68   b : Upper wall surface of flow path 
               70 : Liquid feeding device 
               100 : nucleic acid extraction test device 
               101 : test container 
               102 : Transportation unit 
               106 : Dispenser 
               107 : Magnetic field generation and movement unit 
               108 : Temperature control unit 
               110 : Container main body 
               112 : Main body portion 
               114 : Upper lid member 
               120 : Magnetism collecting chamber (accommodation portion) 
               121 : Cleaning chamber (first accommodation portion) 
               122 : PCR chamber (second accommodation portion) 
               122   a : Inner bottom surface of PCR chamber 
               123 : Detection chamber (third accommodation portion) 
               125 : Chromatographic carrier accommodation portion 
               128 : Chromatographic carrier 
               130 ,  131 ,  132 ,  135 ,  145 : flow path 
               150 : specimen solution 
               151 : Cleaning solution 
               152 : PCR solution 
               153 : Development solution 
               160 - 163 : Syringe 
               170 : Movement mechanism 
               201 : Test container 
               202 : Main body portion 
               202 A: Main body portion 
               202 B: Bottom surface member 
               204 : Upper lid member 
               204 A: Portion 
               205 : One accommodation portion 
               205   b : Upper wall surface of one accommodation portion 
               206 : Other accommodation portion 
               208 : flow path