Patent Publication Number: US-2003235856-A1

Title: Oligonucleotide for detection and identification of mycobacteria

Description:
CROSS REFERENCE TO RELATED APPLICATIONS  
     [0001] This application is a divisional application of U.S. patent application Ser. No. 09/980,052, filed on Nov. 28, 2001, which claims priority to PCT/KR00/00477, filed on May 16, 2000 and is herein incorporated by reference in its entirety. 
    
    
     
       BACKGROUND OF THE INVENTION  
       [0002] 1. Field of the Invention  
       [0003] The present invention relates to oligonucleotides that can be used for detection and identification of mycobacteria. Most particularly, the present invention identifies the nucleotide sequence of ITS (Internal Transcribed Spacer region) of non-tuberculosis mycobacteria,  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi , and using the nucleotide sequences, it provides oligonucleotide primers or probes used for detection and identification of mycobacteria.  
       [0004] 2. Description of the Related Art  
       [0005] Even though the number of patients of tuberculosis has steadily decreased in these days, about 8 million patients have come out and about 3 million patients died of tuberculosis in a year. Moreover, in underdeveloped countries, inadequate treatment and lack of drugs for tuberculosis increase chronic carriers of drug-resistant bacteria. In 1980&#39;s, spread of AIDS has increased patients of tuberculosis even in advanced countries. In this condition, it is expected that about twelve million patents of tuberculosis would newly come out in the year 2000 (J. P. Natain, M. C. Raviglione, and A. Kochi,  Tubercle and Lung Disease,  73; 311-321, 1992; Murray CJL. And Lopez AD. The global burden of disease.  Global burden of disease and injury series . Vol. 1. Cambridge, Mass: Harvard University Press, 1996, p349-350; Global Tuberculosis Programme: Anti-tuberculosis drug resistance, WHO Report 1997: World Health Organization. 1997).  
       [0006] In 1950&#39;s, it was reported that non-tuberculosis mycobacteria (NTM) has been able to cause diseases in human. After the report that  M. avium  complex (MAC) would bring about systemic disease in the patients of AIDS in 1980, non-tuberculosis mycobacteria have taken an interest. Diseases caused by non-tuberculosis mycobacteria are similar to tuberculosis in clinical condition and general pathological view. Non-tuberculosis mycobacteria are distributed in a wide range of living environment, and it is difficult to judge whether they have pathogenicity or not in clinical test sample. Further, since they have resistance to a number of drugs for tuberculosis, the infection is hard to treat and the recurrence rate is high. The infection of non-tuberculosis mycobacteria should be treated by other means than for tuberculosis, and therefore, accurate and fast method of detecting and identifying non-tuberculosis mycobacteria is required. The accurate and fast method of detecting and identifying both TB complex and NTM is also needed for effective treatment and management of tuberculosis.  
       [0007] Many a method has been developed to diagnose mycobacterial infection and to detect and identify mycobacteria strains. Among them, the following methods are used at present:  
       [0008] The first is a microbiological method, that is, smearing, staining and culturing test. However, this method is not suitable for mycobacteria, since they have long generation term and need long culturing time. Further such pathogenic microorganism as mycobacteria is dangerous to infect the personnel in culture room.  
       [0009] The second is a PCR (Polymerase Chain Reaction) method. It is highly sensitive and specific to the mycobacteria and very useful to detect mycobacteria which have a long culturing time. Especially, it does not require a culturing process but uses a small amount of DNA to be amplified, therefore, only a small amount of pathogens in test sample is enough to detect and identify mycobacteria. Many a PCR process has been introduced with different target DNAs each other, and IS6110 and 16S rRNA are often used as the target (Bauer J, Andersen AB, Kremer K, and Miorner H, Usefulness of spoligotyping to discriminate IS6110 low-copy-number Mycobacterium tuberculosis complex strains cultured in Denmark, 1999,  J. Clin Microbiol,  37: 2602-2606; Troesch, A., H. Nguyen, C. G. Miyada, S. Desvarenne, T. R. Gingeras, P. M. Kaplan, P. Cros and C. Mabilat. 1999, Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays,  J. Clin. Microbiol.  37: 49-55);  
       [0010] The third is a physico-chemical process, in which lipid component in mycobacteria has been detected by HPLC, GC or mass spectrophotometer. This method is very specific but requires expensive equipments;  
       [0011] The fourth is a method of detecting mycobacteria composition by serological method. This method uses a coagulation reaction of latex particles or blood corpuscles adsorbed with antibody to mycobacterial antigen or enzyme-linked immunological method in which enzyme is linked with antibody. It is, however, very sensitive only to be proceeded within a limited place. Further, it is difficult for this method to distinguish present infection from previous infection;  
       [0012] The next method to detect mycobacteria consists of infecting mycobacteria with mycobacteriophage L5 inserted with luciferase gene, and inspecting luminescence by luciferin in medium (W. R. Jacobs, R. G. Barletta, R. Udani, J. Chan, G. Kalkut, G. Sosne, T. Kieser, G. J. Sarkis, G. F. Hatful, and B. R. Bloom. 1993, Science 260: 819-822); and  
       [0013] The last is a method of detecting and identifying mycobacteria by hybridization of oligonucleotide (A. Troesch, H. Nguyen, C. G. Miyada, S. Desvarenne, T. R. Gingeras, P. M. Kaplan, P. Cros and C. Mabilat. 1999.  J. Clin. Microbiol.  37: 49-55).  
       [0014] Besides  Mycobacterium avium  complex (MAC) described above,  M. fortuitum, M. chelonae complex, M. terrae  and  M. vaccae  are also known as non-tuberculosis mycobacteria. Among them,  M. chelonae  complex are classified into  M. chelonae  and  M. abscessus , and there is no means to distinguish one from the other at present.  
       SUMMARY OF THE INVENTION  
       [0015] To solve the problems in the prior method of detection and identification of mycobacteria, it is an objective of the present invention to provide specific oligonucleotides as probes or primers for PCR which can be used to detect mycobacteria, to distinguish TB complex from NTM, and to identify species of mycobacteria with an accuracy and effectiveness.  
       [0016] To accomplish the above objective, the present invention provides a DNA of ITS (Internal Transcribed Spacer region) of  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae,    Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi  genes set forth in SEQ ID Nos: 1 to 9.  
       [0017] Further, the present invention provides, as a primer for PCR or a probe for hybridization, an oligonucleotide for detection of mycobacteria set in forth in one of SEQ ID Nos: 10 to 14;  
       [0018] an oligonucleotide for distinction of TB complex from NTB among mycobacteria set in forth in one of SEQ ID NOs: 15 to 23;  
       [0019] an oligonucleotide for detection and identification of MAC ( Mycobacterium avium  and  Mycobacterium intracellulare ) set in forth in one of SEQ ID NOs: 24 to 27;  
       [0020] an oligonucleotide for detection and identification of  Mycobacterium fortuitum  set forth in one of SEQ ID NOs: 28 to 38;  
       [0021] an oligonucleotide for detection and identification of  Mycobacterium chelonae  set forth in one of SEQ ID NOs: 39 to 46;  
       [0022] an oligonucleotide for detection and identification of  Mycobacterium abscessus  set forth in one of SEQ ID NOs: 47 to 52;  
       [0023] an oligonucleotide for detection and identification of  Mycobacterium vaccac  set forth in one of SEQ ID NOs: 53 to 64;  
       [0024] an oligonucleotide for detection and identification of  Mycobacterium flavescens  set forth in one of SEQ ID NOs: 65 to 72;  
       [0025] an oligonucleotide for detection and identification of  Mycobacterium gordonae  set forth in one of SEQ ID NOs: 73 to 77;  
       [0026] an oligonucleotide for detection and identification of  Mycobacterium terrae  set forth in one of SEQ ID NOs: 78 to 100;  
       [0027] an oligonucleotide for detection and identification of  Mycobacterium scrofulaceum  set forth in one of SEQ ID NOs: 101 to 108;  
       [0028] an oligonucleotide for detection and identification of  Mycobacterium kansasii  set forth in one of SEQ ID NOs: 109 to 112;  
       [0029] an oligonucleotide for detection and identification of  Mycobacterium szulgai  set forth in one of SEQ ID NOs: 113 to 116;  
       [0030] an oligonucleotide for detection and identification of  Mycobacterium marinum  and  Mycobacterium ulcerans  set in forth in one of SEQ ID NOs: 117 to 119;  
       [0031] an oligonucleotide for detection and identification of  Mycobacterium gastri  set forth in one of SEQ ID NOs: 120 to 123;  
       [0032] an oligonucleotide for detection and identification of  Mycobacterium xenopi  set forth in one of SEQ ID NOs: 124 to 133;  
       [0033] an oligonucleotide for detection and identification of  Mycobacterium genavense  set forth in one of SEQ ID NOs: 134 to 141;  
       [0034] an oligonucleotide for detection and identification of  Mycobacterium malmoense  set forth in one of SEQ ID NOs: 142 to 146;  
       [0035] an oligonucleotide for detection and identification of  Mycobacterium simiae  set forth in one of SEQ ID NOs: 147 to 153;  
       [0036] an oligonucleotide for detection and identification of  Mycobacterium smegmatis  set forth in one of SEQ ID NOs: 154 to 165;  
       [0037] an oligonucleotide for detection and identification of  Mycobacterium shimoidei  set forth in one of SEQ ID NOs: 166 to 172;  
       [0038] an oligonucleotide for detection and identification of  Mycobacterium habana  set forth in one of SEQ ID NOs: 173 to 180;  
       [0039] an oligonucleotide for detection and identification of  Mycobacterium farcinogen  set forth in one of SEQ ID NOs: 181 to 189;  
       [0040] an oligonucleotide for detection and identification of  Mycobacterium asiaticum  set forth in one of SEQ ID NOs: 190 to 193;  
       [0041] an oligonucleotide for detection and identification of  Mycobacterium porcinum  set forth in one of SEQ ID NOs: 194 to 205;  
       [0042] an oligonucleotide for detection and identification of  Mycobacterium acapulcensis  set forth in one of SEQ ID NOs: 206 to 215;  
       [0043] an oligonucleotide for detection and identification of  Mycobacterium diernhoferi  set forth in one of SEQ ID NOs: 216 to 227;  
       [0044] an oligonucleotide for detection and identification of  Mycobacterium paratuberculosis  set in forth in one of SEQ ID NOs: 228 to 240; and  
       [0045] an oligonucleotide for detection of Mycobacteria sp. set forth in SEQ ID NO: 241.  
       [0046] In the prior method of detecting and identifying mycobacteria using PCR, only one or two strains can be detected. According to the present invention, however, almost all of mycobacteria strains can be detected and identified, since primers and probes of the present invention have been designed from DNA sequences of ITS of mycobacteria. ITS has more polymorphic region than 16S rRNA has and ITS also has conserved region, therefore, it is highly effective as a target DNA for distinction of genotype (Gurtler, V., A. Stanisich, 1996, New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region.  Microbiol.  142: 3-16).  
       [0047] The inventors identified DNA sequences of ITS of non-tuberculosis mycobacteria whose DNA had not yet been sequenced, such as  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi . Using the DNA sequences, oligonucleotides for primers or probes have been designed for detecting and identifying the mycobacteria. Further, referring to the information on DNA sequence of other mycobacteria disclosed in GenBank, and analyzing the information with multi-alignment and blast, distinctive regions of polymorphism were selected to design oligonucleotides for primers or probes to detect and identify mycobacteria. The oligonucleotides have been confirmed to detect and identify mycobacteria by specific hybridization and amplification with species-specific and genus-specific primers of PCR.  
       [0048] That is, the oligonucleotide probes of the present invention, attached to solid substrate, are hybridized only with nucleotide sequence in ITS of specific mycobacteria, and therefore, they can detect and identify the specific mycobacteria sensitively. Further, the oligonucleotide primers of identical nucleotide sequence with the above probes can also detect and identify the specific mycobacteria by amplification in PCR. Using the oligonucleotide primers or probes made from ITS of mycobacteria, it is possible to detect mycobacteria, distinguish TB complex from NTM, and identify mycobacteria species accurately and effectively. 
     
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
     [0049] The above and other objects, features, and advantages of the present invention will be apparent from the following detailed description of the preferred embodiment of the invention in conjunction with the accompanying drawings, in which:  
     [0050]FIG. 1 is a schematic diagram showing ITS of mycobacteria and primers used to amplify the ITS by PCR;  
     [0051]FIG. 2 is a photograph showing the result of electrophoresis after PCR using several mycobacteria strains and a pair of primers for ITS amplification including a part of 16S rRNA and 23S rRNA of mycobacteria;  
     [0052]FIG. 3 is a photograph showing the result of electrophoresis after PCR using several mycobacteria strains and a pair of primers (ITSF and MYC2) for detecting mycobacteria;  
     [0053]FIG. 4 is a schematic diagram of multiplex PCR for detecting mycobacteria and simultaneously distinguishing TB complex from NTM;  
     [0054]FIG. 5 is a photograph showing the result of electrophoresis after multiplex PCR using a pair of primers (ITSF and MYC2) for detecting mycobacteria and a pair of primers (MTB2 and MYC2) for distinguishing TB complex from NTM;  
     [0055]FIG. 6 is a photograph showing the result of electrophoresis after PCR using each mycobacteria and each pair of species-specific primers designed from nucleotide sequence of polymorphic region of each NTM; and  
     [0056]FIG. 7 is a photograph showing the result of electrophoresis after PCR using several mycobacteria and each pair of species-specific primers designed from nucleotide sequence of polymorphic region of each NTM. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION  
     [0057] Now, preferred embodiments of the present invention will be described with reference to the accompanying drawings.  
     EXAMPLE 1  
     Culture of Mycobacteria and Separation of Genome DNA  
     [0058] Standard strains of mycobacteria were obtained from KCTC (Korean Collection for Type Culture) and ATCC (American Type Culture Collection), clinical strains were obtained from the Korean National Tuberculosis Association, National Masan Tuberculosis Hospital and Pusan National University Hospital. The strains were stored and cultured under the management of clinical microbiology specialist in Pusan National University Hospital. The mycobacteria and pathogens used in the examples are shown in Table 8.  
     [0059] Mycobacteria DNA was extracted by the following processes: Mycobacteria were cultured on Ogawa medium. A loopful of cultured strain was put in eppendorf tube, mixed with 200 μl of InstaGene matrix (Bio-Rad Co.) and incubated at 56° C. for 30 minutes. After 10 minutes&#39; vortex missing, the mixture was placed at 100° C. for 8 minutes. After another 10 minutes&#39; vortex mixing, the mixture was centrifuged at 12,000 rpm for 3 minutes. The supernatant was moved to another tube and stored at −20° C. 2 μl of the DNA solution of each strain was used in the succeeding PCR.  
     EXAMPLE 2  
     Manufacture of Primers for Amplifying ITS of Mycobacteria  
     [0060] The ITS of mycobacteria and primers used to amplify the ITS by PCR were illustrated in FIG. 1. The primers for amplifying ITS of mycobacteria were constructed from several section of conserved regions of 16S rRNA and 23S rRNA of mycobacteria. DNA sequences of 16S and 23S rRNA of mycobacteria disclosed in GenBank were analyzed with multialignment and blast search. The primers were designed to amplify selectively about 500 bp of ITS region with a part of 16S rRNA and 23S rRNA and have sequences set in forth in SEQ ID NOs: 242 (ITSF) and 243 (ITSR). All the primers used in Examples were manufacture by the concentration of 50 nmol with Perkin-Elmer DNA Synthesizer by BioBasic (Canada).  
     EXAMPLE 3  
     PCR and Identification of Products  
     [0061] 2 μl of the DNA solution obtained in Example 1 was used in PCR. Reaction solution included: 500 mM KCl, 100 mM Tris HCl (pH 9.0), 1% Triton X-100, 0.2 mM dNTP (dATP, dGTP, dTTP and dCTP), 1.5 mM MgCl 2 , 1 pmol of primer, 1U of Taq DNA polymerase (Bio Basis Inc.). After denaturated at 94° C. for 5 minutes, the solution was reacted 30 cycles of denaturation at 94° C. for 1 minute, annealing at 60° C. for 1 minute, and elongation at 72° C. for 1 minute. The samples were incubated further for 10 minutes at 72° C. for complete elongation. After the reaction, the PCR products were identified by electrophoresis on 1.5% agarose gel. As expected from the information of GenBank, the ITS amplified using the conserved primers of 16S rRNA and 23S rRNA was about 500 bp.  
     [0062]FIG. 2 is a photograph showing the result of electrophoresis after PCR using several mycobacteria strains and a pair of primers for ITS amplification including a part of 16S rRNA and 23S rRNA of mycobacteria. In this figure, M means molecular weight mark having intervals of 100 bp, C is a control group, lanes 1 to 9 indicate, in turn,  M. fortuitum, M chelonae, M. intracellularae, M. avium, M. tuberculosis, M. agri, M. kansasii, M. gordonae, and M. tuberculosis  H37Rv. It can be noted that all mycobacteria of lanes 1 to 9 have amplification of ITS gene except control group C.  
     EXAMPLE 4  
     Determination of DNA Sequence of Mycobacteria ITS  
     [0063] After the identification of PCR products, the reactants of  M. fortuitum, M. chelonae, M. abscessus, M. vaccae, M. fiavescens, M. asiaticum, M. porcinum, M. acapulcensis  and  M. diernhoferi , whose DNA sequences of ITS have not yet been determined, were amplified by PCR. The PCR products were used directly in determining DNA sequence of ITS.  
     [0064] DNA was used by the concentration of 200 μmol. DNA sequence was determined by dye terminator method using universal primer M13 with DNA auto sequencer (Perkin-Elmer ABI prim 377 sequencer).  
     [0065] SEQ ID NOs 1 to 9 indicate DNA sequences of ITS of  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi , in turn.  
     EXAMPLE 5  
     Design and Synthesis of Oligonucleotide Primer  
     [0066] Each primer of about 20 bp was designed from DNA sequences of ITS of  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi , obtained in Example 4, and compared with DNA sequences of ITS of other mycobacteria obtained from GenBank by multi-alignment and blast.  
     [0067] Based on the result, conservative DNA sequences of mycobacteria ITS were designed as primers or probes for detection of mycobacteria, and DNA sequences of high polymorphism were designed as species-specific primers or probes. Tables 1 to 7 depict the designed primers or probes with their position and SEQ ID NOs.  
                           TABLE 1                       Target Strain   Probe Name   Position in ITS   SEQ ID NO.                  Mycobacteria   MYC1   Variable to the   10           MYC2   species   11           MYC3       12           MYC4       13           MYC5       14       TB complex   MTB1   166-185   15           MTB2   65-84   16           MTB3   20-39   17           MTB4   41-60   18           MTB5   60-79   19           MTB6    81-100   20           MTB7   125-144   21           MTB8   139-158   22           MTB9   203-222   23         M. avium-M.     MAC1   241-260   24         intracellularae     MAC2   142-161   25       (MAC)   MAC3    92-111   26           MAC4   117-136   27         M. fortuitum     FOR1   40-59   28           FOR2   44-63   29           FOR3   64-83   30           FOR4   78-97   31           FOR5    89-108   32           FOR6   109-128   33           FOR7   114-133   34           FOR8   134-153   35           FOR9   157-176   36           FOR10   246-265   37           FOR11   289-308   38         M. chelonae     CHE1   11-30   39           CHE2   29-48   40           CHE3   58-77   41           CHE4   78-97   42           CHE5   109-128   43           CHE6   132-151   44           CHE7   171-190   45           CHE8   246-265   46                  
 
     [0068]                           TABLE 2                       Target Strain   Probe Name   Position in ITS   SEQ ID NO.                    M. abscessus     ABC1   37-56   47           ABC2   55-74   48           ABC3   247-266   49           ABC4   263-282   50           ABC5   270-289   51           ABC6   261-280   52         M. vaccae     VAC1   18-37   53           VAC2   38-57   54           VAC3   58-77   55           VAC4   118-137   56           VAC5   138-157   57           VAC6   158-177   58           VAC7   178-197   59           VAC8   199-218   60           VAC9   219-238   61           VAC10   265-284   62           VAC11   298-317   63           VAC12   321-340   64         M. flavescens     FLA1   12-31   65           FLA2   32-51   66           FLA3   52-71   67           FLA4   72-91   68           FLA5   105-124   69           FLA6   125-144   70           FLA7   173-192   71           FLA8   278-297   72         M. gordonae     GOR1    84-103   73           GOR2   249-268   74           GOR3   216-235   75           GOR4   201-220   76           GOR5   223-242   77                    
     [0069]                           TABLE 3                       Target Strain   Probe Name   Position in ITS   SEQ ID NO.                    M. terrae     TER1   178-197   78           TER2   237-256   79           TER3   24-43   80           TER4   70-89   81           TER5    89-108   82           TER6   102-121   83           TER7   122-141   84           TER8   142-161   85           TER9   162-181   86           TER10   182-201   87           TER11   202-221   88           TER12   222-241   89           TER13   238-257   90           TER14   307-326   91           TER15   322-341   92           TER16   342-361   93           TER17   362-381   94           TER18   382-401   95           TER19   12-21   96           TER20   31-50   97           TER21   52-71   98           TER22   72-91   99           TER23    82-101   100          M. scrofulaceum     SCO1   118-137   101            SCO2   131-150   102            SCO3   210-229   103            SCO4    84-103   104            SCO5   152-171   105            SCO6   200-219   106            SCO7   221-240   107            SCO8   241-260   108          M. kansasii     KAN1   35-54   109            KAN2   238-257   110            KAN3    83-102   111            KAN4   214-233   112                     
     [0070]                           TABLE 4                       Target Strain   Probe Name   Position in ITS   SEQ ID NO.                    M. szulgai     SZU1   124-143   113           SZU2   209-228   114           SZU3   227-246   115           SZU4   247-166   116         M. marinum  and   MAR-ULC1    85-104   117         M ulcerans     MAR-ULC2   128-147   118           MAR-ULC3   224-243   119         M. gastri     GAS1    85-104   120           GAS2   145-164   121           GAS3   133-152   122           GAS4   239-258   123         M. xenopi     XEN1   190-209   124           XEN2    1-20   125           XEN3   21-40   126           XEN4   41-60   127           XEN5   61-80   128           XEN6    81-100   129           XEN7   121-140   130           XEN8   141-160   131           XEN9   201-220   132           XEN10   221-240   133         M. genavense     GEN1   190-209   134           GEN2    85-104   135           GEN3   131-150   136           GEN4   147-166   137           GEN5   186-205   138           GEN6   206-225   139           GEN7   226-245   140           GEN8   240-265   141         M. malmoense     MAL1   203-222   142           MAL2   29-48   143           MAL3   136-155   144           MAL4   222-241   145           MAL5   242-261   146         M. simiae     SIM1    83-102   147           SIM2   129-148   148           SIM3   209-227   149           SIM4   22-41   150           SIM5   80-99   151           SIM6   136-155   152           SIM7   241-260   153                    
     [0071]                           TABLE 5                       Target Strain   Probe Name   Position in ITS   SEQ ID NO.                    M. smegmatis     SMEG1   17-36   154           SMEG2   37-56   155           SMEG3   57-76   156           SMEG4   77-96   157           SMEG5    97-116   158           SMEG6   117-136   159           SMEG7   137-156   160           SMEG8   157-176   161           SMEG9   177-196   162           SMEG10   193-212   163           SMEG11   60-80   164           SMEG12   112-131   165         M. shimoidei     SHI1    89-108   166           SHI2   20-39   167           SHI3   70-89   168           SHI4    97-116   169           SHI5   135-154   170           SHI6   224-243   171           SHI7   244-263   172         M. habana     HAB1    86-105   173           HAB2   17-36   174           HAB3   51-70   175           HAB4    81-100   176           HAB5   134-153   177           HAB6   175-194   178           HAB7   200-219   179           HAB8   242-261   180         M. farcinogen     FAR1   122-141   181           FAR2   111-130   182           FAR3   22-41   183           FAR4   48-67   184           FAR5   76-95   185           FAR6   108-127   186           FAR7   114-133   187           FAR8   275-294   188           FAR9   295-314   189                    
     [0072]                           TABLE 6                       Target Strain   Probe Name   Position in ITS   SEQ ID NO.                    M. asiaticum     ASI1    82-101   190           ASI2   145-164   191           ASI3   189-208   192           ASI4   274-293   193         M. porcinum     POR1   45-64   194           POR2   13-32   195           POR3   67-86   196           POR4    91-110   197           POR5   115-134   198           POR6   137-156   199           POR7   164-183   200           POR8   194-213   201           POR9   221-240   202           POR10   273-292   203           POR11   298-317   204           POR12   325-344   205         M. acapulcensis     ACA1   66-85   206           ACA2   112-131   207           ACA3   132-151   208           ACA4   178-197   209           ACA5   198-217   210           ACA6   219-238   211           ACA7   242-261   212           ACA8   262-281   213           ACA9   318-337   214           ACA10   350-369   215         M. diernhoferi     DIE1   16-35   216           DIE2   36-55   217           DIE3   62-81   218           DIE4   103-122   219           DIE5   154-173   220           DIE6   175-194   221           DIE7   195-214   222           DIE8   232-251   223           DIE9   261-280   224           DIE10   282-301   225           DIE11   304-343   226           DIE12   344-363   227                    
     [0073]                           TABLE 7                       Target Strain   Probe Name   Position in ITS   SEQ ID NO.                    M. para-tuberculosis     PARA1    7-26   228           PARA2   30-49   229           PARA3   40-59   230           PARA4   50-59   231           PARA5   71-90   232           PARA6    83-102   233           PARA7   103-122   234           PARA8   135-154   235           PARA9   157-176   236           PARA10   178-197   237           PARA11   198-217   238           PARA12   219-238   239           PARA13   241-260   240       M. sp   SP1   225-244   241                    
     EXAMPLE 6  
     Result of PCR using Primers for detecting Mycobacteria  
     [0074] Genus-specific primers, designed from conserved DNA sequence in mycobacteria, were used for detecting mycobacteria. Among the primers manufactured from the ITS sequence of 270-350 bp, a pair of primers, ITSF (SEQ ID NO. 242) and MYC2 (SEQ ID NO. 11) were used to proceed PCR. As a result, amplified nucleotides of about 350 bp were obtained in mycobacteria strains, while no amplification was occurred in  Staphylococcus aureus, Enterococcus faecium  and  Serratia marcescens . Therefore, it is understood that the primers could be used for detecting mycobacteria.  
     [0075]FIG. 3 is a photograph showing the result of electrophoresis after PCR using several mycobacteria strains and a pair of primers (ITSF and MYC2) for detecting mycobacteria. In this figure, M indicates a size marker of 100 bp ladder; C indicates a negative control; lane 1 indicates  M. abscessus  ATCC 19977; lane 2,  M. agri  ATCC 27406; lane 3,  M. asiaticum  ATCC 25276; lane 4,  M. austroafricanum  ATCC 33464; lane 5,  M. avium  ATCC 25291; lane 6,  M. bovis  ATCC 19210; lane 7,  M. chelonae  ATCC 35752; lane 8,  M. flavescens  ATCC 14474; lane 9,  M. fortuitum  ATCC 6841; lane 10,  M. gordonae  ATCC 14470; lane 11,  M. intracellularae  ATCC 13950; lane 12,  M. kansasii  ATCC 12478; lane 13,  M. phlei  ATCC 354; lane 14,  M. scrofulaceum  ATCC 19981; lane 15,  M. smegmatis  ATCC 21701; lane 16,  M. szulgai  ATCC 35799; lane 17,  M. terrae  ATCC 15755; lane 18,  M. triviale  ATCC 23292; lane 19,  M. tuberculosis  H37Rv; and lane 20,  M. vaccae  ATCC 15483. It can be seen that all mycobacteria of lanes 1 to 20 show ITS amplification except negative control C.  
     [0076] Table 8 shows the results of PCR using a pair of primers (ITSF and MYC2) for detecting mycobacteria. In this table, +indicates amplification occurred and −indicates no amplification. It can be seen that no amplification has occurred in order strains than mycobacteria.  
                           TABLE 8                       Name of Strain   result   Name of Strain   result                    M. tuberculosis  H37Rv   +     M. Phlei  ATCC 354   +         M. bovis  ATCC 19210   +     Aeromonas hydrophila     −         M. avium  ATCC 25291   +     Burkholderia cepacia     −         M intracellulare  ATCC 13950   +     Candida albicans     −         M. abscessus  ATCC 19977   +     Citrobacter freundii     −         M. chelonae  ATCC 35752   +     Enterobacter aerogenes     −         M. flavescens  ATCC 14474   +     Enterobacter cloacae     −         M. fortuitum  ATCC 6841   +     Enterobacter faecalis     −         M. gastri  ATCC 15754   +     Enterobacter faecium     −         M. genavense  ATCC 51233   +     Enterobacter raffinosis     −         M. gordonae  ATCC 14470   +     Escherichia coli     −         M. kansasii  ATCC 12478   +     Klebsiella pneumoniae     −         M. malmoense  ATCC 29571   +     Plesiomonas shigelloides     −         M. scrofulaceum  ATCC   +     Proteus mirabilis     −       19981         M. simiae  ATCC 25275   +     Proteus vulgaris     −         M. smegmatis  ATCC 21701   +     Providencia rettgeri     −         M. szulgai  ATCC 35799   +     Pseudomonas aeruginosa     −         M. terrae  ATCC 15755   +     Rohnella aquatilis     −         M. vaccae  ATCC 15483   +     Salmonella spp.     −         M. xenopi  ATCC 19250   +     Serratia marcescens     −         M. marinum  ATCC 927   +     Shewanella putrefaciens     −         M. ulcerance  ATCC 19423   +     Shigella flexneri     −         M. porcinum  ATCC 33776   +     Shigella sonnei     −         M. asiaticum  ATCC 25276   +     Staphylococcus epidermidis     −         M. acapulcensis  ATCC 14473   +     Staphylococcus aureus     −         M. diernhoferi  ATCC 19340   +     Streptococcus agalactiae     −                  
 
     [0077]                                                M. agri  ATCC 27406   +     Streptococcus intermidius     −         M. austroafricanum  ATCC   +     Streptococcus pneumoniae     −       33464         M. triviale  ATCC 23292   +     Vibrio parahemolyticus     −                    
     EXAMPLE 7  
     Result of PCR using Primers for TB Complex  
     [0078] Test for identifying each strain using the oligonucleotide primers manufactured in the previous Examples was confirmed by amplification in PCR. For identifying TB complex, multiplex PCR was carried using a pair of primers (ITSF and MYC2) for detecting mycobacteria and a pair of primers (MTB 2 and MYC 2; SEQ ID Nos. 16 and 11) for distinguishing TB complex from NTM.  
     [0079]FIG. 4 is a schematic diagram of multiplex PCR for detecting mycobacteria and simultaneously distinguishing TB complex from NTM.  
     [0080]FIG. 5 is a photograph showing the result of electrophoresis after multiplex PCR using a pair of primers (ITSF and MYC2) for detecting mycobacteria and a pair of primers (MTB2 and MYC2) for distinguishing TB complex from NTM. In this figure, M indicates a size marker of 100 bp ladder; C indicates a negative control; lanes 1 and 2 indicate TB complex,  M. tuberculosis  H37Rv and  M. bovis , respectively; and lanes 3 to 10 indicate  M. avium, M. intracellularae, M. fortuitum, M. chelonae, M. gordonae, M. szulgai, M. terrae , and  M. scrofulaceum  ATCC 19981, in turn. In lanes 1 and 2 of TB complex, two bands are formed by double amplification due to primers for detecting mycobacteria and primers for TB complex, while in the order lanes of NTM strains, only one band is formed, which confirms single amplification.  
     EXAMPLE 8  
     Result of PCR using Primers for Identifying NTM Strains  
     [0081] PCR was carried out using species-specific primers manufacture from DNA sequence of polymorphic site of each strain for identifying NTM species.  
     [0082] Specifically, after selecting SEQ ID NOs. 16 and 21 for  M. tuberculosis  H37Rv and  M. bovis , SEC ID NOs: 24 to 27 for  M. avium  and  M. Intracellularae , SEQ ID Nos. 29 and 37 for  M. fortuitum , SEQ ID Nos. 41 and 44 for  M. chelonae , SEQ ID Nos. 48 and 49 for  M. abscessus , SEQ ID Nos. 55 and 63 for  M. vaccac , SEQ ID Nos. 66 and 72 for  M. flavescens , SEQ ID Nos. 73 and 75 for  M. gordonae , SEQ ID Nos. 88 and 96 for  M. terrae , SEQ ID Nos. 102 and 103 for  M. scrofulaceum , SEQ ID Nos. 109 and 110 for  M. kansasii , SEQ ID Nos. 113 and 114 for  M. szulgai , SEQ ID Nos. 117 and 119 for  M. marinum  and  M. ulcerans , SEQ ID Nos. 120 and 123 for  M. gastri , SEQ ID Nos. 128 and 132 for  M. xenopi , SEQ ID Nos. 135 and 141 for  M. genavense , SEQ ID Nos. 143 and 145 for  M. malmoense , SEQ ID Nos. 147 and 149 for  M. simiae , and SEQ ID Nos. 154 and 159 for  M. smegmatis , each mycobacteria was carried out PCR using each pair of primers of which the first has been sense strand and the second has been antisense strand. After the reaction, each resultant was treated by electrophoresis.  
     [0083]FIG. 6 is a photograph showing the result of electrophoresis after PCR using each mycobacteria and each pair of species-specific primers designed from nucleotide sequence of polymorphic region of each NTM. In this figure, M indicates a size marker of 100 bp ladder; C indicates a negative control; lanes 1 and 2 indicate TB complex,  M. tuberculosis  H37Rv and M. bovis ATCC 19210, respectively; and lanes 3 and 4 indicate  M. avium  ATCC 25291 and  M. intracellularae  ATCC 13950, respectively; lane 5 indicates  M. fortuitum  ATCC 6841; lane 6,  M. chelonae  ATCC 35752; lane 7,  M. abscessus  ATCC 19977; lane 8,  M. vaccae  ATCC 15483; lane 9,  M. flavescens  ATCC 14474; lane 10,  M. gordonae  ATCC 14470; lane 11,  M. terrae  ATCC 15755; lane 12,  M. scrofulaceum  ATCC 19981; lane 13,  M. kansasii  ATCC 12478; lane 14,  M. szulgai  ATCC 35799; lane 15,  M. marinum  ATCC 927; lane 16,  M. ulcerans  ATCC 19423; lane 17,  M. gastri  ATCC 15754; lane 18,  M. xenopi  ATCC 19250; lane 19,  M. genavense  ATCC 1233; lane 20,  M. malmoense  ATCC 29571; lane 21,  M. simiae  ATCC 25275; and lane 22 indicates  M. smegmatis  ATCC 21701. Species-specific amplifications can be seen in lanes 1 to 22.  
     [0084]FIG. 7 is a photograph showing the result of electrophoresis after PCR using several mycobacteria and each pair of species-specific primers designed from nucleotide sequence of polymorphic region of each NTM. In this figure, the first photograph using the pair of primers (ITSF and MYC2) specific for TB complex shows amplification in lanes 1 and 2 of  M. tuberculosis  H37Rv and  M. bovis ; the second using the pair of primers (MAC1 and MAC4) specific for  M. avium  and  M. intracellularae  shows amplifications in lanes 3 and 4 of  M. avium  and  M. intracellularae ; the third using the pair of primers (FOR2 and FOR10) specific for  M. fortuitum  shows amplification in lane 5 of  M. fortuitum ; the fourth using the pair of primers (CHE3 and CHE6) specific for  M. chelonae  shows amplification in lane 6 of  M. chelonae ; the fifth using the pair of primers (GOR1 and GOR2) specific for  M. gordonae  shows amplification in lane 7 of  M. gordonae ; the sixth using the pair of primers (SZU1 and SZU2) specific for  M. szulgai  shows amplification in lane 8 of  M. szulgai ; and the seventh using the pair of primers (SCO1 and SCO2) specific  M. scrofulaceum  for shows amplification in lane 10 of  M. scrofulaceum . Lane 9 indicates  M. terrae , which shows no amplification with the above species-specific primer pairs.  
     [0085] Therefore, PCR using each pair of species-specific primers can detect and identify specifically each species of mycobacteria.  
     [0086] As described above, identifying DNA sequences of ITS (Internal Transcribed Spacer region) of non-tuberculosis mycobacteria,  Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium vaccae, Mycobacterium flavescens, Mycobacterium asiaticum, Mycobacterium porcinum, Mycobacterium acapulcensis  and  Mycobacterium diernhoferi , and using the DNA sequences, oligonucleotide primers or probes can been designed for detecting and identifying mycobacteria. Using the primers for PCR or probes for hybridization, it is possible to detect mycobacteria, distinguish TB complex from NTM and identify mycobacteria species with rapidity and effectiveness. Such detection and identification method makes the diagnosis of complex infection effective, and therefore, it is possible to treat accurately mycobacterial infection including tuberculosis.  
     [0087] While this invention has been described in connection with what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiment, but, on the contrary, it is intended to cover various modifications and equivalent arrangements included with the spirit and scope of the appended claims.  
 
    
     
       
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                                                SEQUENCE LISTING  
               
               
                   
               
               
                   
               
               
                 &lt;160&gt; NUMBER OF SEQ ID NOS: 243  
               
               
                 &lt;210&gt; SEQ ID NO 1  
               
               
                 &lt;211&gt; LENGTH: 386  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 1  
               
               
                   
               
               
                 aggagcacca cgaaaagggt tgagacactg ggtcttaccc gagccgtgag gaaccggttg     60  
               
               
                   
               
               
                 cctgtagtgg gcacggtttg gtgcacaaca aacttttttg actgccagac acactattgg    120  
               
               
                   
               
               
                 gctttgagac aacaggcccg tgcccctttt ggggggtggc atccggttgc gggtgtcggc    180  
               
               
                   
               
               
                 gtgttgttgc ctcactttgg tggtggggtg tggtgtttga tttgtggata gtggttgcga    240  
               
               
                   
               
               
                 gcatctagca cgcagaatcg tgtggtctca ctccttgtgg gtggggctgg ttttgtgtgt    300  
               
               
                   
               
               
                 tgatgtgcaa tttcttttga aactcatttt tggtttttgt gttgtaagtg tttaagggcg    360  
               
               
                   
               
               
                 catggtggat gccttggcag gatcca                                         386  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 2  
               
               
                 &lt;211&gt; LENGTH: 338  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 2  
               
               
                   
               
               
                 aggagcacca tttcccagcc gaatgagctt gggaacataa agcgagtttc tgtagtggtt     60  
               
               
                   
               
               
                 actcgcttgg tgaatatgtt ttataaatcc tgtccacccc gtggataggt agtcggcaaa    120  
               
               
                   
               
               
                 acgtcggact gtcaatagaa ttgaaacgct ggcacactgt tgggtcctga ggcaacacat    180  
               
               
                   
               
               
                 tgtgttgtca ccctgcttgg tggtggggtg tggtctttga cttatggata gtggttgcga    240  
               
               
                   
               
               
                 gcatctaaca aacctcgctc gtttacgagt gaggttagtt tttgcaattt attagctaag    300  
               
               
                   
               
               
                 ttcttaaggg cacatggtgg atgccttggc aggatcca                            338  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 3  
               
               
                 &lt;211&gt; LENGTH: 317  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium abscessus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 3  
               
               
                   
               
               
                 aaggagcacc atttcccagt cgaatgaact agggaacata aagtaggcat ctgtagtgga     60  
               
               
                   
               
               
                 tatctacttg gtgaatatgt tttgtaaatc ctgtccaccc cgtggatggg tagtcggcaa    120  
               
               
                   
               
               
                 aacgtcggac tgtcataaga attgaaacgc tggcacactg ttgggtcccg aggcaacacg    180  
               
               
                   
               
               
                 ttgtgttgtc accctgcttg gtggtggggt gtggactttg acttctgaat agtggttgcg    240  
               
               
                   
               
               
                 agcatctaaa catagcctcg ctcgttttcg agtggggctg gtttttgcaa ttttattagc    300  
               
               
                   
               
               
                 taagttctta agggcgc                                                   317  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 4  
               
               
                 &lt;211&gt; LENGTH: 429  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 4  
               
               
                   
               
               
                 aaggagcacc acgagaatca ggcccgccca catcgtgtgg gggttcggtg atctgatcga     60  
               
               
                   
               
               
                 ttcgttggat ggcctttcat ctgtagtgga tgggggtctg gtgcacatga caaacttggc    120  
               
               
                   
               
               
                 cgagccggta gggaatgccg gcgagggaaa tcatcagaca cactattggg ctttgagaca    180  
               
               
                   
               
               
                 acaagcccgt gccccttttt ttgggggtgg ctctgcgttg gcagggtcgg cgtgttgttg    240  
               
               
                   
               
               
                 ccccgctttg gtggtggggt gtggtgtttg attcgtggat agtggttgcg agcatctgaa    300  
               
               
                   
               
               
                 tgcacagcgc ttgtggtgtt gtgtgttcgg tgtaatgcaa atttttctga tactcgcatg    360  
               
               
                   
               
               
                 cagtcccttt ttgggggtgt gtgtgggtga ctcatttttt ggttttgtgt tgtaagtgtt    420  
               
               
                   
               
               
                 taagggcgc                                                            429  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 5  
               
               
                 &lt;211&gt; LENGTH: 379  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 5  
               
               
                   
               
               
                 aaggagcacc atttattgtt cccccgtccc cacgtgtgtg ggatcagtgc ggttgggaga     60  
               
               
                   
               
               
                 tcagtgccgg gcctgtagtg ggtttccggt gggtgcacaa caaacgtgag aagtggtgtg    120  
               
               
                   
               
               
                 ggaacactgc tttgaggaat catcagacac actattgggc tttgaggcaa caggcccgtt    180  
               
               
                   
               
               
                 gtttccctgg ccactgtgtg tggtgggggg tctggtgtcg ccctgtcttt ggtggtgggg    240  
               
               
                   
               
               
                 tgtggtgttt gattcgtgga tagtggttgc gagcatctga acaggtggct cccttttggg    300  
               
               
                   
               
               
                 ggttgcttgt tttgataatg caatttttta ttcttccgag aatatttttc tgttttgtgt    360  
               
               
                   
               
               
                 tgtaagtgtt taagggcgc                                                 379  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 6  
               
               
                 &lt;211&gt; LENGTH: 364  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium asiaticum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 6  
               
               
                   
               
               
                 tggatccgac gaagtcgtaa caaggtagcc gtaccggaag gtgcggctgg atcacctcct     60  
               
               
                   
               
               
                 ttctaaggag caccacgaaa gcatcccaat tggtgggatg caggccgtgt ggagttctcg    120  
               
               
                   
               
               
                 tctgtagtgg acgagggctg ggtgcacaac aacaagcaag ccagacacac tattgggtcc    180  
               
               
                   
               
               
                 tgagacaaca ctcgggcgct agcacgaagt gttgtccctc catcttggtg gtggggtgtg    240  
               
               
                   
               
               
                 gtgtttgaga actggatagt ggttgcgagc atcaactgat cgcgtcgccg ttcgcggtgg    300  
               
               
                   
               
               
                 cgtgttcttt tgtgcaattt taaattcttt ggtttttgta gtgtttgtaa gtgtctaagg    360  
               
               
                   
               
               
                 gcgc                                                                 364  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 7  
               
               
                 &lt;211&gt; LENGTH: 402  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 7  
               
               
                   
               
               
                 aaggagcacc gattcgattc ccccgccgtc cgcgtagtcg tgggcagtag tgcggttggg     60  
               
               
                   
               
               
                 atatttcagc caggacctgt agtgggtgtc tggtgggtgc aaatgacaaa cgttgagccg    120  
               
               
                   
               
               
                 gcgcgggaaa gcgttggtga tggaactgct gaacacacta ttgggctttg agacaacagg    180  
               
               
                   
               
               
                 cccgtgcccc tttcgggggg tggcattccg ttgggagtgt cggcgtgttg ttgctccgct    240  
               
               
                   
               
               
                 ttggtggtgg ggtgtggtgt ttgatttgtg gatagtggtt gcgagcatct agcacgcagt    300  
               
               
                   
               
               
                 gtggctgggg gccttcgggt ttccggtctt gttgtgtgtt gatgtgcaat ttcttttgaa    360  
               
               
                   
               
               
                 actcattttt ggtttttgtg ttgtaagtgt ttaagggcgc at                       402  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 8  
               
               
                 &lt;211&gt; LENGTH: 459  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 8  
               
               
                   
               
               
                 aaggagcacc agttattttc ccccgccccc acgcctgtgg gatcagttgc ggttgggata     60  
               
               
                   
               
               
                 tttagtgcca gcacctgtag tgggtgtttc ggccggtgca cagcaaacgt tgatcgtctg    120  
               
               
                   
               
               
                 gtggggaaag ccgggcgtga aagattgcca gacacactat tgggctttga gacaacaagc    180  
               
               
                   
               
               
                 ccgtcgcctc tttgtcccga gtgtgggata tcggagaagg agcaccacga gacctgttgc    240  
               
               
                   
               
               
                 ccgcccacat cgtgtgggag ttcggtgact caggcgattc gggggttggt tgtggttgtc    300  
               
               
                   
               
               
                 gccctgcttt ggtggtgggg tgtggtgttt gatttgtgga tagtggttgc gagcatctga    360  
               
               
                   
               
               
                 acgcagagac ctgtgtgggt ttttgtgttc tgataatgca atttttattc ttccgagaat    420  
               
               
                   
               
               
                 attttttgat ctgttttgtg tgtaagtgtt taagggcgc                           459  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 9  
               
               
                 &lt;211&gt; LENGTH: 418  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE  
               
               
                   
               
               
                 aaggagcacc accctg ttgcccgccc acatcgtgtg ggagttcggt gactcaggcg         60  
               
               
                   
               
               
                 attcgttgga ttcaca cctgtagtgg gtgggggtct ggtgcacaac aaactttgag        120  
               
               
                   
               
               
                 aaactgccag actatt gggctttgag acaacaggcc ctgcggtgcc ggactcgttg        180  
               
               
                   
               
               
                 gagtcctggt tgccgc gagtcccgga agcgattctg gttcggacgg tgtctgttgt        240  
               
               
                   
               
               
                 tgctccatct ttgtgg ggtgtggtgt ttgatttgtg gatagtggtt gcgagcatct        300  
               
               
                   
               
               
                 agcacgcaag agctgg gtttccttcg ggagcccggg tttttgttgt gtgtgtttga        360  
               
               
                   
               
               
                 tgtgcaattt ttttcttcta tttggtttta tctgtgttgt aagtgtttaa gggcgcat      418  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 10  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacteria  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 10  
               
               
                   
               
               
                 tggtggggtg tggtgtttga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 11  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacteria  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 11  
               
               
                   
               
               
                 tggatagtgg ttgcgagcat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 12  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacteria  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 12  
               
               
                   
               
               
                 ccatcttggt ggtggggtgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 13  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacteria  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 13  
               
               
                   
               
               
                 cacactattg ggccctgagg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 14  
               
               
                 &lt;211&gt; LENGTH: 10  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacteria  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 14  
               
               
                   
               
               
                 aaggagcacc                                                            10  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 15  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 15  
               
               
                   
               
               
                 cactcggact tgttccaggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 16  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 16  
               
               
                   
               
               
                 tggtggggcg taggccgtga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 17  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 17  
               
               
                   
               
               
                 ccccaactgg tggggcgtag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 18  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 18  
               
               
                   
               
               
                 ccgtgagggg ttcttgtctg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 19  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 19  
               
               
                   
               
               
                 gtagtgggcg agagccgggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 20  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 20  
               
               
                   
               
               
                 catgacaaca aagttggcca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 21  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 21  
               
               
                   
               
               
                 caacactcgg acttgttcca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 22  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 22  
               
               
                   
               
               
                 gttccaggtg ttgtcccacc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 23  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g TB complex  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 23  
               
               
                   
               
               
                 cgagcatcaa tggatacgct                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 24  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium avium complex(MAC)  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 24  
               
               
                   
               
               
                 gttcatcgaa atgtgtaatt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 25  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium avium complex(MAC)  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 25  
               
               
                   
               
               
                 gtgtggagtc cctccatctt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 26  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium avium complex(MAC)  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 26  
               
               
                   
               
               
                 aaatgattgc cagacacact                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 27  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium avium complex(MAC)  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 27  
               
               
                   
               
               
                 ccctgagaca acactcggtc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 28  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 28  
               
               
                   
               
               
                 cgagccgtga ggaaccggtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 29  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 29  
               
               
                   
               
               
                 ccgtgaggaa ccggttgcct                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 30  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 30  
               
               
                   
               
               
                 gtagtgggca cggtttggtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 31  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 31  
               
               
                   
               
               
                 ttggtgcaca acaaactttt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 32  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 32  
               
               
                   
               
               
                 caaacttttt tgactgccag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 33  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 33  
               
               
                   
               
               
                 acacactatt gggctttgag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 34  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 34  
               
               
                   
               
               
                 ctattgggct ttgagacaac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 35  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 35  
               
               
                   
               
               
                 aggcccgtgc cccttttggg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 36  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 36  
               
               
                   
               
               
                 tggcatccgg ttgcgggtgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 37  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 37  
               
               
                   
               
               
                 tagcacgcag aatcgtgtgg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 38  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium fortuitum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 38  
               
               
                   
               
               
                 ggttttgtgt gttgatgtgc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 39  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 39  
               
               
                   
               
               
                 tttcccagcc gaatgagctt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 40  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 40  
               
               
                   
               
               
                 ttgggaacat aaagcgagtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 41  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 41  
               
               
                   
               
               
                 gttactcgct tggtgaatat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 42  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 42  
               
               
                   
               
               
                 gttttataaa tcctgtccac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 43  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 43  
               
               
                   
               
               
                 gtagtcggca aaacgtcgga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 44  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 44  
               
               
                   
               
               
                 tcaatagaat tgaaacgctg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 45  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 45  
               
               
                   
               
               
                 ggcaacacat tgtgttgtca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 46  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium chelonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 46  
               
               
                   
               
               
                 taacaaacct cgctcgttta                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 47  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium abscessus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 47  
               
               
                   
               
               
                 cataaagtag gcatctgtag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 48  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium abscessus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 48  
               
               
                   
               
               
                 agtggatatc tacttggtga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 49  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium abscessus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 49  
               
               
                   
               
               
                 taaacatagc ctcgctcgtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 50  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium abscessus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 50  
               
               
                   
               
               
                 cgttttcgag tggggctggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 51  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium abscessus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 51  
               
               
                   
               
               
                 gagtggggct ggtttttgca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 52  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium abscessus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 52  
               
               
                   
               
               
                 ctcgttttcg agtggggctg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 53  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 53  
               
               
                   
               
               
                 tcaggcccgc ccacatcgtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 54  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 54  
               
               
                   
               
               
                 tgggggttcg gtgatctgat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 55  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 55  
               
               
                   
               
               
                 cgattcgttg gatggccttt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 56  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 56  
               
               
                   
               
               
                 ggccgagccg gtagggaatg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 57  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 57  
               
               
                   
               
               
                 ccggcgaggg aaatcatcag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 58  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 58  
               
               
                   
               
               
                 acacactatt gggctttgag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 59  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 59  
               
               
                   
               
               
                 acaacaagcc cgtgcccctt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 60  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 60  
               
               
                   
               
               
                 ttttgggggt ggctctgcgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 61  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 61  
               
               
                   
               
               
                 tggcagggtc ggcgtgttgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 62  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 62  
               
               
                   
               
               
                 tgtttgattc gtggatagtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 63  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 63  
               
               
                   
               
               
                 gaatgcacag cgcttgtggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 64  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecti  
               
               
                       ng Mycobacterium vaccae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 64  
               
               
                   
               
               
                 gtgtgttcgg tgtaatgcaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 65  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 65  
               
               
                   
               
               
                 tttattgttc ccccgtcccc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 66  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 66  
               
               
                   
               
               
                 acgtgtgtgg gatcagtgcg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 67  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 67  
               
               
                   
               
               
                 gttgggagat cagtgccggg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 68  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 68  
               
               
                   
               
               
                 cctgtagtgg gtttccggtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 69  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 69  
               
               
                   
               
               
                 cgtgagaagt ggtgtgggaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 70  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 70  
               
               
                   
               
               
                 cactgctttg aggaatcatc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 71  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 71  
               
               
                   
               
               
                 ggcccgttgt ttccctggcc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 72  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium flavescens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 72  
               
               
                   
               
               
                 tgaacaggtg gctccctttt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 73  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gordonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 73  
               
               
                   
               
               
                 cgacaacaag ctaagccaga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 74  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gordonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 74  
               
               
                   
               
               
                 tgttcttttt gtgcaatttt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 75  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gordonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 75  
               
               
                   
               
               
                 gcatcaaaat gtatgcgttg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 76  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gordonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 76  
               
               
                   
               
               
                 aaaatgtatg cgttgtcgtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 77  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gordonae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 77  
               
               
                   
               
               
                 cggcaacgtg ttctttttgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 78  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 78  
               
               
                   
               
               
                 ttgagttgtg gatagtggtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 79  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 79  
               
               
                   
               
               
                 gtcgagtgtt tagagagtaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 80  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 80  
               
               
                   
               
               
                 cgcactgggc gcattccgag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 81  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 81  
               
               
                   
               
               
                 gtgggggctg ggtgcacaac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 82  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 82  
               
               
                   
               
               
                 caacgttgaa aacaagatcg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 83  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 83  
               
               
                   
               
               
                 aattacgaac aacaacaagc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 84  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 84  
               
               
                   
               
               
                 ttgcgagatc atcaactgcc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 85  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 85  
               
               
                   
               
               
                 aggagtcctt gggggtttct                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 86  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 86  
               
               
                   
               
               
                 ggtggccggc ttttgtgctg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 87  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 87  
               
               
                   
               
               
                 ggcacactgt tgggtcctga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 88  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 88  
               
               
                   
               
               
                 ggcaacaggc ccgtttgtgc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 89  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 89  
               
               
                   
               
               
                 ccccgggtgg gggtgggtgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 90  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 90  
               
               
                   
               
               
                 gtgttgttgt cgcctcacac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 91  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 91  
               
               
                   
               
               
                 taacaagcag atttttggtc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 92  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 92  
               
               
                   
               
               
                 tggtctgttt gttttgcaat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 93  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 93  
               
               
                   
               
               
                 ttttgtttct tggtttttgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 94  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 94  
               
               
                   
               
               
                 gtttgtaagt gtttaagggc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 95  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 95  
               
               
                   
               
               
                 gcatggtgga tgccttggca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 96  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 96  
               
               
                   
               
               
                 ctttttcccc cgtgcctcac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 97  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 97  
               
               
                   
               
               
                 atgggtgagg gtttttgcgg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 98  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 98  
               
               
                   
               
               
                 ttgggacagt gtttgccggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 99  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 99  
               
               
                   
               
               
                 gcctgtagtg ggtggccggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 100  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detectin  
               
               
                       g Mycobacterium terrae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 100  
               
               
                   
               
               
                 ggtggccggt ggtgcagagg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 101  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium scrofluceum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 101  
               
               
                   
               
               
                 cctgaggcaa cactcggctc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 102  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium scrofluceum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 102  
               
               
                   
               
               
                 tcggctcgtt ctgagtggtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 103  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium scrofluceum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 103  
               
               
                   
               
               
                 taaacggatg cgtggccgaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 104  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium scrofluceum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 104  
               
               
                   
               
               
                 caacagcaaa tgattgccag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 105  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium scrofluceum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 105  
               
               
                   
               
               
                 ccctccatct tggtggtggg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 106  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium scrofluceum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 106  
               
               
                   
               
               
                 tgcgagcatc taaacggatg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 107  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium scrofluceum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 107  
               
               
                   
               
               
                 gtggccgaac ggtggcgtgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 108  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium scrofluceum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 108  
               
               
                   
               
               
                 tcgttgaaat gtgtaatttc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 109  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium kansasii  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 109  
               
               
                   
               
               
                 gtggggtgca agccgtgagg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 110  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium kansasii  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 110  
               
               
                   
               
               
                 gcgtgttctt ttgtgcaatt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 111  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium kansasii  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 111  
               
               
                   
               
               
                 gcaactgtaa atgaatcacc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 112  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium kansasii  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 112  
               
               
                   
               
               
                 ctggatgcgc tgccgttcgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 113  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium szulgai  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 113  
               
               
                   
               
               
                 aacactcagg cttggccaga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 114  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium szulgai  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 114  
               
               
                   
               
               
                 caattggatg cgctgccctc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 115  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium szulgai  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 115  
               
               
                   
               
               
                 tcgtggtggc gtgttctttt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 116  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium szulgai  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 116  
               
               
                   
               
               
                 gtgcaatttt aattctttgg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 117  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium marinum and Mycobacterium ulcerans  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 117  
               
               
                   
               
               
                 aacaacaagc aagccagaca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 118  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium marinum and Mycobacterium ulcerans  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 118  
               
               
                   
               
               
                 atctctgttg gtttcgggat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 119  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium marinum and Mycobacterium ulcerans  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 119  
               
               
                   
               
               
                 ccttttggtg gcgtgttctg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 120  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gastri  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 120  
               
               
                   
               
               
                 aacagcaagc aagccagaca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 121  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gastri  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 121  
               
               
                   
               
               
                 ctcgtccaag agtgttgtcc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 122  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gastri  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 122  
               
               
                   
               
               
                 gcttgtcttg gactcgtcca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 123  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium gastri  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 123  
               
               
                   
               
               
                 cagggtagcg tgttcttttg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 124  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 124  
               
               
                   
               
               
                 catctggcaa agactgtggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 125  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 125  
               
               
                   
               
               
                 agggagcacc gtaaacgcat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 126  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 126  
               
               
                   
               
               
                 cccgcgtggg gtggggtgtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 127  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 127  
               
               
                   
               
               
                 ggttcggcgt gttgtggcgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 128  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 128  
               
               
                   
               
               
                 cgggccgagg tgttgggcag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 129  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 129  
               
               
                   
               
               
                 caggcagtaa ccgccggcac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 130  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 130  
               
               
                   
               
               
                 caggcagtaa ccgccggcac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 131  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 131  
               
               
                   
               
               
                 tccgcgtggt ggcggggtgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 132  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 132  
               
               
                   
               
               
                 agactgtggt aagcggtttt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 133  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium xenopi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 133  
               
               
                   
               
               
                 tgttgagtgt tttctggtgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 134  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium genavense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 134  
               
               
                   
               
               
                 cattgaatag tggttgcgag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 135  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium genavense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 135  
               
               
                   
               
               
                 aacaacaggc aatcgccgga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 136  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium genavense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 136  
               
               
                   
               
               
                 tcggccgact gaggtcgacg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 137  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium genavense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 137  
               
               
                   
               
               
                 gacgtggtgt ccctccatct                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 138  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium genavense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 138  
               
               
                   
               
               
                 ttgagcattg aatagtggtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 139  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium genavense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 139  
               
               
                   
               
               
                 gcgagcatct agacggatgc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 140  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium genavense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 140  
               
               
                   
               
               
                 gttccccagt ggtgcgcgtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 141  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium genavense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 141  
               
               
                   
               
               
                 cgtcaaaaat gtgtaatttt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 142  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium malmoense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 142  
               
               
                   
               
               
                 agcatctaaa cggatgcgct                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 143  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium malmoense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 143  
               
               
                   
               
               
                 tggtggggtg caagccgtga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 144  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium malmoense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 144  
               
               
                   
               
               
                 ccagtccgcg tggtgtcccc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 145  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium malmoense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 145  
               
               
                   
               
               
                 tgcccgtaga cgcgtattcg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 146  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium malmoense  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 146  
               
               
                   
               
               
                 tttgtgtaat ttcttctttg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 147  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium simiae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 147  
               
               
                   
               
               
                 acaacaacag gcaatcgcca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 148  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium simiae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 148  
               
               
                   
               
               
                 actcggccga cttcggttga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 149  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium simiae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 149  
               
               
                   
               
               
                 gagcatctaa atgaacgcgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 150  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium simiae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 150  
               
               
                   
               
               
                 tccaattggt ggggtgtgag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 151  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium simiae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 151  
               
               
                   
               
               
                 tgcacaacaa caggcaatcg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 152  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium simiae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 152  
               
               
                   
               
               
                 cgacttcggt tgaagtggtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 153  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium simiae  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 153  
               
               
                   
               
               
                 tacgtgttcg ttttgtgtaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 154  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 154  
               
               
                   
               
               
                 gacactctcc gttggggagg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 155  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 155  
               
               
                   
               
               
                 gtgtgagccg tgaggagctg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 156  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 156  
               
               
                   
               
               
                 gagcgctgta gtggcgccgg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 157  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 157  
               
               
                   
               
               
                 cttggtgcac agcaaacgtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 158  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 158  
               
               
                   
               
               
                 gagatgcggt gtgggaaacg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 159  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 159  
               
               
                   
               
               
                 ctgtttcgat ggactgccag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 160  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 160  
               
               
                   
               
               
                 acacactatt gggccctgag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 161  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 161  
               
               
                   
               
               
                 acaacaggcc cgcgttcccg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 162  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 162  
               
               
                   
               
               
                 tcccgttggg ggcggggggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 163  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 163  
               
               
                   
               
               
                 gggtgtgttg ttgccctgct                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 164  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 164  
               
               
                   
               
               
                 gctgtagtgg cgccggcttg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 165  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium smegmatis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 165  
               
               
                   
               
               
                 aaacgctgtt tcgatggact                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 166  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium shimoidei  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 166  
               
               
                   
               
               
                 aacaacaagc gagaagccga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 167  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium shimoidei  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 167  
               
               
                   
               
               
                 ggcttccgca gtgggcggaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 168  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium shimoidei  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 168  
               
               
                   
               
               
                 gggcgcgggc tgggtgcaca                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 169  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium shimoidei  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 169  
               
               
                   
               
               
                 gcgagaagcc gagcacactg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 170  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium shimoidei  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 170  
               
               
                   
               
               
                 cccgggccct ttggggttgg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 171  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium shimoidei  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 171  
               
               
                   
               
               
                 aagcaaaact tggttgtttt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 172  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting Myc  
               
               
                       obacterium shimoidei  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 172  
               
               
                   
               
               
                 gtttgtcgag ttgttttctt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 173  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium habana  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 173  
               
               
                   
               
               
                 aacaacaggc aatcgccaga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 174  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium habana  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 174  
               
               
                   
               
               
                 aacactccaa ttgggtgggg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 175  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium habana  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 175  
               
               
                   
               
               
                 ggttctcgtc tgtagtggac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 176  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium habana  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 176  
               
               
                   
               
               
                 tgcacaacaa caggcaatcg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 177  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium habana  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 177  
               
               
                   
               
               
                 ggccgacttc ggttgaagtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 178  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium habana  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 178  
               
               
                   
               
               
                 ggggtgtggt gtttgagtat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 179  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium habana  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 179  
               
               
                   
               
               
                 gagcatctaa atgaacgcgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 180  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium habana  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 180  
               
               
                   
               
               
                 tacgtgttcg ttttgtgtaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 181  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for  
               
               
                       detecting Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 181  
               
               
                   
               
               
                 gttggtgatg gactgccaga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 182  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 182  
               
               
                   
               
               
                 cgcgggaaag cgttggtgat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 183  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 183  
               
               
                   
               
               
                 cgatgtcgtg ggcagtagtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 184  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 184  
               
               
                   
               
               
                 ggatatttca gccagcatct                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 185  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 185  
               
               
                   
               
               
                 tgtctggtgg gtgcaaatga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 186  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 186  
               
               
                   
               
               
                 cggcgcggga aagcgttggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 187  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 187  
               
               
                   
               
               
                 gggaaagcgt tggtgatgga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 188  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 188  
               
               
                   
               
               
                 tctagcacgc agagtgtggc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 189  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium farcinogen  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 189  
               
               
                   
               
               
                 tgggggcctt cgggtttctt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 190  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium asiaticum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 190  
               
               
                   
               
               
                 catcccaatt ggtgggatgc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 191  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium asiaticum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 191  
               
               
                   
               
               
                 cacaacaaca agcaagccag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 192  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium asiaticum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 192  
               
               
                   
               
               
                 cactcgggcg ctagcacgaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 193  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium asiaticum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 193  
               
               
                   
               
               
                 aactgatcgc gtcgccgttc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 194  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 194  
               
               
                   
               
               
                 cagtagtgcg gttgggatat                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 195  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 195  
               
               
                   
               
               
                 ttcgattccc ccgccgtccg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 196  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 196  
               
               
                   
               
               
                 cagccaggac ctgtagtggg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 197  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 197  
               
               
                   
               
               
                 tggtgggtgc aaatgacaaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 198  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 198  
               
               
                   
               
               
                 gagccggcgc gggaaagcgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 199  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 199  
               
               
                   
               
               
                 gtgatggaac tgctgaacac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 200  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 200  
               
               
                   
               
               
                 ggctttgaga caacaggccc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 201  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 201  
               
               
                   
               
               
                 cggggggtgg cattccgttg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 202  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 202  
               
               
                   
               
               
                 cggcgtgttg ttgctccgct                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 203  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 203  
               
               
                   
               
               
                 tagtggttgc gagcatctag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 204  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 204  
               
               
                   
               
               
                 agtgtggctg ggggccttcg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 205  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                        Mycobacterium porcinum  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 205  
               
               
                   
               
               
                 ggtcttgttg tgtgttgatg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 206  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacteriun acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 206  
               
               
                   
               
               
                 tgccagcacc tgtagtgggt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 207  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 207  
               
               
                   
               
               
                 gatcgtctgg tggggaaagc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 208  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 208  
               
               
                   
               
               
                 cgggcgtgaa agattgccag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 209  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 209  
               
               
                   
               
               
                 agcccgtcgc ctctttgtcc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 210  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 210  
               
               
                   
               
               
                 cgagtgtggg atatcggaga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 211  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 211  
               
               
                   
               
               
                 ggagcaccac gagacctgtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 212  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 212  
               
               
                   
               
               
                 cgcccacatc gtgtgggagt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 213  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 213  
               
               
                   
               
               
                 tcggtgactc aggcgattcg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 214  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 214  
               
               
                   
               
               
                 gggtgtggtg tttgatttgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 215  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium acapulcensis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 215  
               
               
                   
               
               
                 cgagcatctg aacgcagaga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 216  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 216  
               
               
                   
               
               
                 acctgttgcc cgcccacatc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 217  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 217  
               
               
                   
               
               
                 gtgtgggagt tcggtgactc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 218  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 218  
               
               
                   
               
               
                 ttcgttggat ggcctcacac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 219  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 219  
               
               
                   
               
               
                 tgcacaacaa actttgagaa                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 220  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 220  
               
               
                   
               
               
                 acaggccctg cggtgccgga                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 221  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 221  
               
               
                   
               
               
                 tcgttggagt cctggttgcc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 222  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 222  
               
               
                   
               
               
                 ggccgcgagt cccggaagcg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 223  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 223  
               
               
                   
               
               
                 gtctgttgtt gctccatctt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 224  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 224  
               
               
                   
               
               
                 ggtgtggtgt ttgatttgtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 225  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 225  
               
               
                   
               
               
                 atagtggttg cgagcatcta                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 226  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 226  
               
               
                   
               
               
                 acgcaagagg agtctgggtt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 227  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium diernhoferi  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 227  
               
               
                   
               
               
                 ttgttgtgtg tgtttgatgt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 228  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 228  
               
               
                   
               
               
                 gagcaccacg aaaagcaccc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 229  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 229  
               
               
                   
               
               
                 ctggtggggt gcgagccgtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 230  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 230  
               
               
                   
               
               
                 gcgagccgtg aggggttccc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 231  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 231  
               
               
                   
               
               
                 aggggttccc gtctgtagtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 232  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 232  
               
               
                   
               
               
                 acgggggccg ggtgcgcaac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 233  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 233  
               
               
                   
               
               
                 caaatgattg ccagacacac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 234  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 234  
               
               
                   
               
               
                 tattgggcct gagacaacac                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 235  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 235  
               
               
                   
               
               
                 cggtccgtcc gtgtggagtc                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 236  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 236  
               
               
                   
               
               
                 tccatcttgg tggtggggtg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 237  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 237  
               
               
                   
               
               
                 ggtgtttgag tattggatag                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 238  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 238  
               
               
                   
               
               
                 tggttgcgag catctagatg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 239  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 239  
               
               
                   
               
               
                 gcgcatggtc tccgtggccg                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 240  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacterium paratuberculosis  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 240  
               
               
                   
               
               
                 gttcatcgaa atgtgtaatt                                                 20  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 241  
               
               
                 &lt;211&gt; LENGTH: 20  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of probe or primer for detecting  
               
               
                       Mycobacteri  
               
               
                 &lt;400&gt; SEQUENCE: 241  
               
               
                   
               
               
                 aaaagctgtt gtttgacggt                                                 20  
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 242  
               
               
                 &lt;211&gt; LENGTH: 25  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of primer for amplifying Mycobacteria  
               
               
                       ITS selected from the sequence of 16S rRNA in Mycobacteria  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 242  
               
               
                   
               
               
                 tggatccgac gaagtcgtaa caagg                                           25  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 243  
               
               
                 &lt;211&gt; LENGTH: 25  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: sequence of primer for amplifying Mycobacteria  
               
               
                       ITS selected from the sequence of 23S rRNA in Mycobacteria  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 243  
               
               
                   
               
               
                 tggatcctgc caaggcatcc accat                                           25