Patent Publication Number: US-2020297047-A1

Title: Non-chemical, mosquito bite-resistant garments

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a Continuation of U.S. application Ser. No. 16/039,628, having the title, “NON-CHEMICAL, MOSQUITO BITE-RESISTANT GARMENTS”, filed on Jul. 19, 2018, which application claims the benefit of and priority to U.S. Provisional Application Ser. No. 62/534,663, having the title “NON-CHEMICAL, MOSQUITO BITE-RESISTANT GARMENTS”, filed on Jul. 19, 2017, the disclosure of each which is incorporated herein by reference in their entireties. 
    
    
     TECHNICAL FIELD 
     The present disclosure generally relates to garments, and in particular to undergarments that are mosquito bite-resistant. 
     BACKGROUND 
     A great number of protective garment teachings or particular combinations of protective features which have been developed over a long period of years. One example is U.S. Pat. No. 4,685,152 which teaches a heavy, tightly sealed outer garment intended for insect protection, and this type of protective garment also includes absolute protection about the head and face as it includes an isolating visor and head closure member. This device is an outer garment wherein tightness of closure is a prime feature. U.S. Pat. No. 3,496,572 provides teaching of another form of outer garment that provides tight closure in protection of the human body. In this case, the suit is intended for dust-proof maintenance of the wearer. This patent too is directed to a very bulky outer garment with a plurality of straps and cinches about selected body areas and including a visor and full head protector. Wrist and ankle security is further protected by multiple wraps of isolation material. 
     U.S. Pat. No. 4,601,066 teaches a more form-fitting type of outer garment of the type that is intended for use by dancers, gymnasts and acrobats. Here again, the wrists and ankles are wrapped with multiple folds in order to provide warming at the extremities and neck of the wearer. 
     Prior-art protective undergarments do not prevent mosquito bites. There remains a need for improved undergarments that overcome these deficiencies. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       Further aspects of the present disclosure will be readily appreciated upon review of the detailed description of its various embodiments, described below, when taken in conjunction with the accompanying drawings. 
         FIGS. 1A-B  are views of exemplary undergarments. 
         FIG. 2  is a section view of a waist band for top and bottom of an exemplary undergarment showing an exemplary means of joinder. 
         FIG. 3  is a section view of a waist band for top and bottom of an exemplary undergarment showing an exemplary means of joinder. 
         FIG. 4  is a section view of a selected portion for top and bottom members of an exemplary undergarment including a cotton liner feature. 
         FIGS. 5A-5D  are sectional views of an exemplary top ( FIGS. 5A, 5C ) and exemplary bottom ( FIGS. 5B, 5D ) demonstrating embodiments including reinforcement applied to the elbow and knee respectively. 
         FIGS. 6A-6I  show components of in vitro blood feeding device: ( FIG. 6A ) body of the device showing the blood reservoir and stainless steel heating coils, ( FIG. 6B ) collagen film, ( FIG. 6C ) cork gasket, ( FIG. 6D ) plastic frame, ( FIGS. 6E-6G ) valves and stem, ( FIG. 6H ) clips, and ( FIG. 6I ) PVC collar and clamp for holding the device. 
         FIGS. 7A-7B  show blood-feeding device with components assembled ( FIG. 7A ) and inserted into a bioassay cage ( FIG. 7B ). 
     
    
    
     DETAILED DESCRIPTION 
     In various aspects, form-fitting insect-proof protective undergarments are provided. The undergarments can include a synthetic knit material made with ultrafine fibers and providing mosquito bite resistance. For example, the synthetic knit materials can provide a mosquito bite resistance of about 99% or more or about 100% when measured according to the in vivo feeding bioassay described herein. 
     Before the present disclosure is described in greater detail, it is to be understood that this disclosure is not limited to particular embodiments described, and as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting. The skilled artisan will recognize many variants and adaptations of the embodiments described herein. These variants and adaptations are intended to be included in the teachings of this disclosure and to be encompassed by the claims herein. 
     All publications and patents cited in this specification are cited to disclose and describe the methods and/or materials in connection with which the publications are cited. All such publications and patents are herein incorporated by references as if each individual publication or patent were specifically and individually indicated to be incorporated by reference. Such incorporation by reference is expressly limited to the methods and/or materials described in the cited publications and patents and does not extend to any lexicographical definitions from the cited publications and patents. Any lexicographical definition in the publications and patents cited that is not also expressly repeated in the instant specification should not be treated as such and should not be read as defining any terms appearing in the accompanying claims. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present disclosure is not entitled to antedate such publication by virtue of prior disclosure. Further, the dates of publication provided could be different from the actual publication dates that may need to be independently confirmed. 
     Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present disclosure, the preferred methods and materials are now described. Functions or constructions well-known in the art may not be described in detail for brevity and/or clarity. Embodiments of the present disclosure will employ, unless otherwise indicated, techniques of material science, textiles engineering and the like, which are within the skill of the art. Such techniques are explained fully in the literature. 
     It should be noted that ratios, concentrations, amounts, and other numerical data can be expressed herein in a range format. It is to be understood that such a range format is used for convenience and brevity, and thus, should be interpreted in a flexible manner to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited. To illustrate, a numerical range of “about 0.1% to about 5%” should be interpreted to include not only the explicitly recited values of about 0.1% to about 5%, but also include individual values (e.g., 1%, 2%, 3%, and 4%) and the sub-ranges (e.g., 0.5%, 1.1%, 2.2%, 3.3%, and 4.4%) within the indicated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the disclosure, e.g. the phrase “x to y” includes the range from ‘x’ to ‘y’ as well as the range greater than ‘x’ and less than ‘y’. The range can also be expressed as an upper limit, e.g. ‘about x, y, z, or less’ and should be interpreted to include the specific ranges of ‘about x’, ‘about y’, and ‘about z’ as well as the ranges of ‘less than x’, less than y′, and ‘less than z’. Likewise, the phrase ‘about x, y, z, or greater’ should be interpreted to include the specific ranges of ‘about x’, ‘about y’, and ‘about z’ as well as the ranges of ‘greater than x’, greater than y′, and ‘greater than z’. In some embodiments, the term “about” can include traditional rounding according to significant figures of the numerical value. In addition, the phrase “about ‘x’ to ‘y’”, where ‘x’ and ‘y’ are numerical values, includes “about ‘x’ to about ‘y’”. 
     Definitions 
     Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the specification and relevant art and should not be interpreted in an idealized or overly formal sense unless expressly defined herein. 
     The articles “a” and “an,” as used herein, mean one or more when applied to any feature in embodiments of the present invention described in the specification and claims. The use of “a” and “an” does not limit the meaning to a single feature unless such a limit is specifically stated. The article “the” preceding singular or plural nouns or noun phrases denotes a particular specified feature or particular specified features and may have a singular or plural connotation depending upon the context in which it is used. 
     The term “form-fitting,” as used herein, refers to materials and garments which are compliant and which will readily conform to the general shape and contours of the wearer&#39;s body. 
     The term “undergarment,” as used herein, refers to a garment designed to be worn next to the body or a portion thereof and regardless of whether additional garments are worn on top thereof. 
     Undergarments 
     A variety of undergarments are provided. The undergarments can include one or both of an upper body form-fitting garment and a lower body form-fitting garment. The garments can be insect proof and/or protective. The garments can provide for mosquito bite resistance, especially when worn together. 
     The undergarment can include an upper body, form-fitting garment (also described as an upper body portion). The upper body form-fitting garment can have a torso section having an elastic neck cuff secured to define a neck opening of the torso section and an elastic waist cuff secured to define a waist band around the torso section. The neck can include a collar such as, e.g. a high, turtle-type knit collar, or crew neck. The upper body form-fitting garment can have a left arm section and a right arm section, each of the arm sections having an inner terminus attached to the torso section and an outer terminus having an elastic wrist cuff. The arm sections can be tubularly seamed in conventional manner and joined to a top part of the torso section by opposite diagonal shoulder seams. 
     The undergarment can include a lower body, form-fitting garment (also described as a lower body portion). The lower body, form-fitting garment can include a waist section having an elastic waist cuff secured to define a waist band around the waist section. The lower body, form-fitting garment can include a left leg section and a right leg section, each of the leg sections having an inner terminus attached to the waist section and an outer terminus having an elastic ankle cuff. The lower body, form-fitting garment can include a fly flap disposed vertically in the waist section with a fastener means aligned therealong to effect closure. The lower body, form-fitting garment can include a fastener means for securing the elastic waist cuff of the upper body, form-fitting garment and the elastic waist cuff of lower body, form-fitting garment to secure the torso section waist band to the waist section waist band. 
     The upper body, form-fitting garment and the lower body, form-fitting garment can be configured to be joined and/or to overlap when being worn so as to provide a continuous protection from insects and mosquito bites. For example, the upper body form-fitting garment can have a shirt tail extending from the upper garment torso section in extension below the elastic waist cuff so that the elastic waist cuffs of the upper and lower body portions interlock over the shirt tail. 
     In various embodiments, the upper body, form-fitting garment and the lower body, form-fitting garments can be configured to be joined by a fastener means e.g. hook and loop, zippers, snaps, buttons, ties, drawstrings, or other clothing fasteners known in the art. For example, the undergarments can include a hook and loop fastener for securing, for example by having a first strip of hook fastener secured around the outer surface of the waist band around the torso section and a second strip of loop fastener secured around the outer surface of the waist band around the waist section, whereby the first and second strips are configured to be joined together. 
     The upper body, form-fitting garment and the lower body, form-fitting garment can be integrated as a single garment so as to provide a continuous protection from insects and mosquito bites. For example, the upper body, form-fitting garment and the lower body, form-fitting garment can be part of a continuous garment (e.g. a union suit). The single form-fitting garment can include a fly flap and/or a rear flap. The single form-fitting garment can also include a vertical closure for ease of donning and removing. Each of the flaps and closures can be secured by fastener means such as hook and loop configurations, zippers, snaps, buttons, ties, drawstrings, or other clothing fasteners and combinations thereof known in the art. 
     The upper body, form-fitting garment and the lower body, form-fitting garment include a variety of elastic cuffs. The cuffs can provide enhanced insect protection and prevent entry of insects there through when in use by the wearer. Each of the cuffs can be suitably formed as by knitting to include sufficient elasticity to assure firm fitting without any discomfort. The undergarment or portions thereof can further include a liner material, although not necessary, to ensure bite protection, and that can provide improved comfort in some aspects. For example, a cotton liner material can be provided in one or more sections of the undergarment. The undergarment can further include reinforcing materials in the joint areas. For example, the undergarment can include reinforcing material added by stitching to the elbows of the upper garment part arm sections and/or the lower garment part leg sections. 
     One or both, preferably both, of the upper body, form-fitting garment and the lower body, form-fitting garment can be made entirely or partially from a synthetic knit material designed to provide enhanced mosquito bite resistance. The synthetic knit material can include about 70% to 90%, about 75% to 80%, or about 80% by weight of a polyamide fiber based upon a total weight of the synthetic knit material. The polyamide fiber can have a fiber thickness of about 15 to 25, about 18 to 22, or about 20 denier count. The synthetic knit material can include about 10% to 30%, about 15% to 25%, or about 20% of an elastane fiber based upon the total weight of the synthetic knit material. The elastane fiber can have a fiber thickness of about 10 to 20, about 12 to 18, or about 15 denier count. 
     The synthetic knit material can be lightweight and/or stretchable. For example, the synthetic knit material can have a weight of about 2.5 to 3.0, about 2.6 to 2.9, or about 2.8 ounces per square yard. The synthetic knit material can have a stretch in a width of the synthetic knit material of about 200% to 260%, about 220% to 260%, about 230% to 250%, or about 240%. The synthetic knit material can have a stretch in a length of the synthetic knit material of about 130% to 190%, about 140% to 190%, about 140% to 180%, or about 160%. The synthetic knit material can have a jersey plated knit structure having about 80-90, about 82-86, or about 84 wales and about 110-120, about 110-114, or about 112 courses per inch, and an average pore size of about 20 μm to 40 μm, about 25 μm to 30 μm, or about 27 μm to 34 μm. 
     The synthetic knit materials and undergarments made therefrom can provide an enhanced level of mosquito bite resistance. The mosquito bite resistance of the synthetic knit materials and undergarments made therefrom can be tested using in vivo and arm-in-cage test methods described herein or variations thereof. The bite resistance can be reported as a percentage bite resistance as described herein. In some aspects, the synthetic knit materials provide a mosquito bite resistance of about 95%, about 98%, about 99%, about 99.5%, or more when measured according to the in vivo feeding bioassay described herein. In some aspects, the synthetic knit materials provide a mosquito bite resistance of about 100% when measured according to the in vivo feeding bioassay described herein. The synthetic knit materials and undergarments made therefrom can provide a mosquito bite resistance of about 95%, about 98%, about 99%, about 99.5%, or more when measured according to the arm-in-cage test described herein. In some aspects, the synthetic knit materials and undergarments made therefrom provide a mosquito bite resistance of about 100% when measured according to the arm-in-cage test described herein. 
     In Vivo Feeding Bioassay 
     The synthetic knit materials can be tested in an in vitro blood-feeding device ( FIGS. 6A-6I and 7A-7B ). The in vitro blood-feeding device ( FIGS. 6A-6I and 7A-7B ) was constructed from Plexiglas®. Briefly, a reservoir for blood (16.5 cm length×3.5 cm width×0.5 cm depth) was produced by a hand-held router in a rectangular piece of Plexiglas (28 cm length×5.5 cm width×1 cm thickness). A hole (4 mm dia.) was drilled at the center of the top and bottom edge through the plastic into the blood reservoir. A tap was used to cut threads into the plastic so that a valve could be screwed into the top and bottom holes. Two holes (each 4 mm dia.) were drilled from the bottom edge of the device through the plastic to the blood reservoir. A loop of stainless steel tubing (3 mm dia.) was placed into the blood reservoir, and the tubing was inserted through the holes so that the cut ends protruded out of the plastic. Epoxy cement was used to seal the tubing in place inside the blood reservoir of the device. The ends of the tubing were connected to a circulating water bath to heat the blood. 
     To setup for a blood feeding bioassay, a transparent collagen film (product code 894010.95; Devro, Inc., Columbia, S.C. USA) was hydrated in distilled water and stretched over the top of the device. A gasket, cut from a sheet of cork-rubber composite (Fel-Pro, part no. 3019; AutoZone, Raleigh, N.C. USA) was placed on top of the collagen film. A rectangular piece of plastic (3 mm thick) the size of the blood-feeding device was then placed on top of the gasket. The central area of both the rubber gasket and plastic frame was removed so that the collagen film is fully exposed. Metal binder clips hold the gasket and frame in place on top of the blood-feeding device, preventing leakage of blood. A 30 mL syringe filled with blood was then attached to the valve that was screwed into the top hole of the blood-feeding device. Wth the device tilted at a slight downward angle the blood was slowly transferred into the reservoir. The valve attached to the bottom of the device opened to allow air displaced by the blood to escape. When the device was filled, both valves were closed, and the circulating water bath was turned on to warm the blood to 35° C. 
     The synthetic knit materials to be evaluated for bite resistance are cut to exactly fit over the collagen film within the plastic frame. Masking tape, placed around the inner edges of the plastic frame, slightly overlaps the cloth. In this way, mosquitoes are prevented from gaining access to the collagen film by probing around the edges of the cloth. The blood-feeding device was inserted into a Plexiglas bioassay cage (30×30 cm on each side) containing mosquitoes. 
     Prior to inserting the blood-feeding device, 25 female mosquitoes were transferred to the bioassay cage.  Aedes aegypti  females were starved overnight (sugar water removed from their rearing cage; females not blood fed) prior to testing, while  Anopheles gambiae  females were starved for only 4 h before each bioassay. Female mosquitoes from 4-10 days of age (post emergence) were used for bioassays. Porcine blood obtained from a local abattoir was used in our bioassays. At the time of collection, sodium citrate was added as an anticoagulant. Just prior to initiating the bioassay, ATP (Sigma) was added to the blood (2.5 mg/mL blood) as a phagostimulant (Benzon and Apperson 1987). Each bioassay was conducted for 10 min., during which the number of times females land and probe the cloth was counted. A single event was recorded if a female landed and then inserted or attempted to insert her proboscis into the cloth, regardless of whether the female probed multiple times after landing. A video recording was made of each bioassay so that the mosquitoes&#39; responses to the surface of each cloth and probing behavior could be studied. At the end of the exposure period, mosquitoes were removed and killed in a freezer. Subsequently, each mosquito was crushed on a sheet of white paper to determine if she was able probe through the cloth and obtain a blood meal. In vitro bioassays were repeated for each candidate fabric a minimum of 3 times with each mosquito species and the appropriate controls (no cloth, a control cloth excised from a military garment with no bite protection, and a control cloth excised from a military garment with bite protection). Calculations for percentage bite resistance and statistical analyses are described later. 
     Arm-In-Cage Studies 
     The synthetic knit materials and undergarments made therefrom can also be tested in arm-in-cage studies and compared to controls. In vivo assays were carried out with informed consent using a protocol for use of human subjects in research that has been approved by the NC State University Institutional Review Board (IRB#2925). A sleeve device was constructed to bioassay textiles that were found to be bite resistant in in vitro assays. The sleeve was constructed from a polyvinyl coated roofing membrane, Sarnafil® (Sika Corp., Canton, Mass. USA). The sleeve was cut into a trapezoidal shape with a 16.5 cm×3.5 cm opening in the center that corresponds to the size and shape of the opening in the in vitro blood-feeding device. A plastic frame was riveted to the sleeve to keep the exposure area from deforming when the sleeve was attached to the forearm of the study participant. 
     Female mosquitoes are transferred to a bioassay cage 10-30 minutes before bioassays are started. A candidate cloth was laid on the underside of a forearm of a study participant. The sleeve was laid on top of the cloth and attached to the participant&#39;s forearm with Velcro® straps. The hand of the participant was then covered with a nitrile glove. The bioassay was started when the participant inserted his/her arm through the sleeve into the bioassay cage. An observer counted the numbers of mosquitoes landing on the cloth and probing during a 10 min. exposure period. After the bioassay was terminated, mosquitoes were examined for blood feeding as previously described. The mosquito conditioning, the numbers of mosquitoes used and level of replication were the same as that described for the in vitro assay. 
     Data Analysis 
     Percentage bite resistance for each test cloth were calculated as follows: (control count-treatment count/control count)×100 for each of the controls, i.e., the bare skin and skin covered by bite permissive cloth. The results were be compared to that for an example of a military textile in use designed for bit protection, i.e., permethrin treated uniform material. Percentage bite resistance data were transformed as needed to achieve approximate normality and then analyzed using a repeated measures ANOVA, with unstructured error covariance. To determine whether statistically significant differences in mean percentage bite resistance (P=0.05) exist between the treatment and controls across all time points and at each time point, probability of difference values were calculated in LSD tests for LSM bit resistance under the hypothesis Ho: LSM(i)=LSM(j) (SAS Institute 2003). 
     The textile can be effective via a physical barrier that can prevent insect bites or a non-toxic treatment that can render the insect harmless without contaminating the environment or posing a potential health risk. These textile materials can mitigate the incidence of insecticide resistance in the vector population and provide an effective alternative to current chemically treated materials. In addition to clothing, there are a number of other cloth items that can benefit from advanced non-chemical insect-resistant treatments, such as mosquito netting, camouflage helmet covers, ground covers, and tentage. 
     EXAMPLES 
     In general, the following Examples describe some additional embodiments of the present disclosure. While embodiments of the present disclosure are described in connection with the following examples and the corresponding text and figures, there is no intent to limit embodiments of the present disclosure to this description. On the contrary, the intent is to cover all alternatives, modifications, and equivalents included within the spirit and scope of embodiments of the present disclosure. 
     Referring to  FIG. 1A , an undergarment  10  includes an upper (shirt) part  12  and a bottom (pants) part  14 , each of which can be formed from a selected synthetic plastics material that has desirable attributes as to weight, stretch and closeness of weave. The shirt part  12  includes right and left arms  16  and  18  which can terminate in respective knit cuffs  20  and  22 . The shirt part  12  can also include a knit collar  24 , which can be a high, turtle-type, and/or a waist cuff  26 . Each of the cuffs can be suitably formed as by knitting to include sufficient elasticity to assure firm fitting without any discomfort. The arms  16  and  18  can be tubularly seamed in a conventional manner and joined to shirt part  12  by opposite diagonal shoulder seams. 
     It is desirable that the material of shirt part  12  be a selected material woven from low denier yarn which exhibits qualities of very lightweight and omnidirectional stretch so that a good, comfortable form-fit is achieved. Generally speaking, any of a number of nylon stretch materials may be used; however, practice has shown that a material known as Glistenette available from Minnetonka Mills, Inc. of Minneapolis, Minn., is quite good for the present use. This material consists of 90 percent ANTRON™ nylon and 10 percent LYCRA™(Spandex) and it is formed in a spandex type weave to exhibit a weight of about 3.5 ounces per square yard. The material has stretch ability in all directions and a close but not totally opaque weave. That is, there is sufficient pass-through space in the weave to permit free air circulation, yet prevent the passage of insects, even minute insects such as the common chigger. 
     The bottom part  14  is formed from the same material to include form-fitting right and left legs  28  and  30  which terminate with respective knit ankle cuffs  32  and  34 . The top part of bottom part  14  terminates in a waist cuff  36  (as will be described in  FIG. 2 ) which is tightly retained beneath outer waist cuff  26  for security purposes. The bottom part  14  also includes a front fly  38  which is suitably closed by means of either a zipper or a VELCRO® pile/hooks fabric combination, a hook and loop fastener. A snap-on hook device (not shown) can be fastened at the point of waist cuff joinder on the front fly  38 . Conventional pant leg seaming may be employed and bottom part  14  can be formed with a diamond panel crotch inset to minimize the possibility of crotch seam splitting. 
       FIG. 1B  shows another possible embodiment of an undergarment. An undergarment  10  includes an upper (shirt) part  12  and a bottom (pants) part  14  as described in  FIG. 1A . The shirt part  12  includes right and left arms  16  and  18  which can terminate in respective knit cuffs  20  and  22 , which can be elongated to cover part of the hands and may include optional thumbholes. The shirt part  12  can also include a knit collar  24 . The bottom part  14  is formed from the same material to include form-fitting right and left legs  28  and  30  which terminate with respective ankle stirrups  33  and  35 , which can be worn over the user&#39;s feet to minimize exposure of the ankle to insects and the environment. Said ankle stirrups  33  and  35  can be worn under socks or tucked into footwear. The top part of bottom part  14  terminates in a waist cuff  36 . Areas of reinforcement (as described in  FIGS. 5A-D ) such as reinforcing elbow ply  62 , stitching  64 , reinforcement panels  66 , and stitching  68  may be included. Additional areas of reinforcement, such as belly or chest panels (not shown) may also be included. 
       FIG. 2  shows one form of fabric combination for securing the top and bottom parts  12  and  14 . The outer waist cuff  36  of bottom part  14  may include a VELCRO® hook and loop fastener or pile strip  40  sewn completely around the inside of waist cuff  36 . In like manner, a VELCRO® hook strip  42  is sewn completely around the outer side of knit cuff  26  of the upper part  12 . When the upper and bottom parts  12  and  14  are donned by the wearer, s/he can then place the outer knit waist cuff  26  in super-position over lower waist cuff  36  so that the pile strip  40  and hook strip  42  meshes around the circumference thereby to effect an insect-tight joinder of the top and bottom undergarment pieces. 
     Alternative securing structure for joining the top part  12  to bottom part  14  is shown in  FIG. 3 . Thus, the top part  12  may include a slightly lower waist cuff  26   a  which, when tucked in, provides an interlocking relationship to the waist cuff  36 . In addition, the top part  12  is formed to extend additional shirttail material  44  beneath waist cuff  26   a  to assure further that no break occurs between top part  12  and bottom part  14  during extended activity. 
       FIG. 4  shows an alternative form of construction wherein the material of the upper part  12   a  and lower part  14   a  are simply stitched around their respective waist bands. That is, the upper part  12   a  material is folded totally around the waist cuff or elastic member  46  and secured by stitching  48 . In like manner, the material of lower part  14   a  is folded around the waist cuff elastic member  50  and secured by stitching  52 . Other security provisions such as those shown in  FIGS. 2 and 3  may also be included in order to maintain integrity of insect-proof coverage. 
       FIG. 4  also shows the inclusion, in both bottom and top parts  12   a  and  14   a , of a thin cotton material liner  54  and  56 , respectively. The upper cotton liner  54  and lower cotton liner  56  may be extended throughout the entire torso, leg and body portions for the purpose of providing greater comfort in certain conditions. The lining using very light cotton fabric could add considerable heat insulation quality while further assuring insect protection, and it would be particularly desirable in cooler weather. 
       FIGS. 5A-5D  illustrate a method of reinforcement which also may be included. Thus,  FIG. 5A  shows an arm  58  with cuff  60  and also includes a reinforcing elbow ply  62  of material which is secured by stitching  64 . More than one ply  62  may be included in the reinforcement panel if desired. As shown in  FIGS. 5B and 5D , similar reinforcement panels  66  and stitching  68  may be included in the lower parts of the undergarment on leg part  70  for knee protection. As shown in  FIG. 1B , reinforcing elbow ply  62 , stitching  64 , reinforcement panels  66 , and stitching  68  may be included in various embodiments. 
     The foregoing discloses a form of undergarment that provides protection against discomforts that one may encounter during outdoor, field activity. The undergarment is a form-fitting, coverall that is constructed from very lightweight, elastic material, and it functions quite favorably for outdoor activities of such as the military, hunters, fishermen and other outdoor disposed activities. 
     It should be emphasized that the above-described embodiments of the present disclosure are merely possible examples of implementations, and are set forth only for a clear understanding of the principles of the disclosure. Many variations and modifications may be made to the above-described embodiments of the disclosure without departing substantially from the spirit and principles of the disclosure. All such modifications and variations are intended to be included herein within the scope of this disclosure.