Patent Publication Number: US-PP33182-P3

Title: Hemp plant named ‘Painted Lady’

Description:
FIELD OF THE INVENTION 
     Botanical classification:  Cannabis  hybrid. 
     Variety denomination: ‘Painted Lady’. 
     BACKGROUND OF THE INVENTION 
     The present invention comprises a new and distinct hemp cultivar, hereinafter referred to by the cultivar name, ‘Painted Lady’. It is noted that “hemp” is a term used to describe varieties of  Cannabis  that contain 0.3% or less Tetrahydrocannabinol (THC) content by dry weight. 
     The inventor, Jeremy Klettke, discovered the new cultivar as a result of crossing two stabilized open source (unpatented) parents, hybridized from the species  Cannabis sativa  L. and  Cannabis indica . More specifically, ‘Painted Lady’ was discovered through a series of outdoor field trials carried out in 2016, 2017, and 2018, combined with a number of parallel indoor pollination projects at Mr. Klettke&#39;s breeding facility in LaPine, Oreg. ‘Painted Lady’ was the result of working with more than 12,000 individual variations of this hybrid. 
     Further developed in a closed environment breeding program, carried out indoors, ‘Painted Lady’ is characterized as a high yielding cultivar for Cannabidiol (CBD) production containing very low quantities of THC, Cannabigerol (CBG), Resorcinol, 2-(3,7-dimethyl-2,6-octadienyl)-5-pentyl, Cannabichromenic Acid (CBC-A), 5-hydroxy-2methyl-2-(4-methylpent-3-enyl)-7-pentyl-chromene-6-carboxylic acid. 
     Asexual reproduction of the new cultivar has been done by stem propagation or cutting performed at Mr. Klettke&#39;s breeding facility in LaPine, Oreg. The unique features of this cultivar are stable and reproduced true to type through successive generations. This is a cultivar bred for plant resin production, containing quantifiable amounts of cannabinoids and terpenes with little to no psychoactivity. 
     SUMMARY OF THE INVENTION 
     In the following description, color references are made to The Royal Horticultural Society Colour Chart (R.H.S. Colour Chart), 6 th  edition, 2015, except where general terms of ordinary dictionary significance are used. 
     The term “cultivar” is used herein interchangeably with “variety” “strain,” and/or “clone.” 
     The cultivar ‘Painted Lady’ has not been observed under all possible environmental conditions. The phenotype may vary somewhat with variations in environment such as temperature, day length, and light intensity, without, however, any variance in genotype. 
     The following traits have been repeatedly observed and are determined to be the unique characteristics of ‘Painted Lady’. These characteristics in combination distinguish ‘Painted Lady’ as a new and distinct hemp cultivar:
         1. This plant is stable and quick growing.   2. This plant displays strong pest and disease resistance.   3. This plant is highly frost resistant, and resistant to powdery mildew ( Podosphaera xanthii ), grey mold or botrytis ( Botrytis cinerea ), and white mold ( Sclerotinia sclerotiorum ).   4. This plant contains high quantities of the compound CBD and low quantities of THC or any other intoxicating compound, with a median CBD to THC ratio of 30:1. The range percentage by weight of the CBD content is 7-9%, and the range percentage by weight of the THC content is 0.2-0.3%.   5. This plant contains significantly higher quantities of the terpenes beta-Myrcene, beta-Caryophyllene, Ocimene Isomer II, alpha-Pinene, beta-Pinene, alpha-Humulene, alpha-Bisabolol, Limonene, and endo-fenchyl alcohol than either of its parents. As determined by liquid chromatography, the range percentages by weight of the terpenes are 0.75-3.5%.   6. This plant has low hermaphroditic potential in the female plant.   7. This plant displays strong tendencies to branch without traditional horticultural manipulation techniques.   8. This plant displays a unique non-alternating branching pattern in cloned plant examples.       

    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       Colors in the photographs may appear different from the color values that appear in the detailed botanical description which more accurately describe the new cultivar. 
       The photographs were taken using conventional techniques and, although colors may appear different from actual colors due to light reflectance, it is as accurate as possible by conventional photographic techniques. 
         FIG. 1  is a top view of a plant of ‘Painted Lady’, showing a developed water leaf. 
         FIG. 2  is a side view of a plant of ‘Painted Lady’, showing a cross-section of a cut stem. 
         FIG. 3  is a side view of a plant of ‘Painted Lady’, showing an immature, partially developed flower. 
         FIG. 4  is a side view of a plant of ‘Painted Lady’, showing a mature, developed flower. 
         FIG. 5  is a side view of a plant of ‘Painted Lady’, showing a whole, mature plant. 
     
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     This invention relates to a new hemp plant hybrid of  Cannabis sativa  L. and  Cannabis indica  named ‘Painted Lady’. ‘Painted Lady’ is a plant bred for indoor and outdoor production of plant resin containing high quantities of the compound CBD and low quantities of THC or any other intoxicating compound. The present plant was developed over 3.5 years at Mr. Klettke&#39;s breeding facility in LaPine, Oreg., using parents selected from open source unstabilized dioecious seed and propagated cuttings. The plant can be grown in most climates through a normal summer growing season from 38-48 degrees of latitude either North or South of the equator. Although this plant is primarily dependent on the photoperiod for flowering, it can be grown throughout a large part of the year in latitudes within 10 degrees of the equator.
     Parentage:
             Seed .—Unnamed  Cannabis  hybrid ‘Oracle’ x ‘Otto II’ #103.     Pollen .—Unnamed  Cannabis  hybrid ‘Oracle’ x ‘Otto II’ #259.       
       

     The parent plants of ‘Painted Lady’ consist of a mother and a father developed from an elite, open source clone named ‘Oracle’ crossed with an unstabilized open source male named ‘Otto II’ which was selected from seed. ‘Oracle’ is a stable resin producing female with a CBD to THC ratio of 20:1 and has been available in limited circles of  Cannabis  cultivators and breeders. ‘Oracle’ is a short growing bushy plant with 1-1.5 inch internodal spacing with moderately broad leaf structure. ‘Otto II’ was developed and released in seed form as a CBD producing hemp cultivar for outdoor production. ‘Otto II’ contains small but measurable amounts of terpenes and very little THC with ratios of CBD to THC ranging from 15:1 through 25:1. 
     The claimed plant ‘Painted Lady’ has been stabilized and has a median CBD to THC ratio of 30:1. ‘Painted Lady’ also contains significantly higher quantities of terpenes than either of its parents and has been bred to avoid hermaphroditic potential in the female plant. 
     
       
         
           
               
               
             
               
                 TABLE I 
               
               
                   
               
             
            
               
                 LATIN  
                   Cannabis  hybrid 
               
               
                 NAME OF  
                   
               
               
                 THE 
                   
               
               
                 FAMILY,  
                   
               
               
                 GENUS AND 
                   
               
               
                 SPECIES 
                   
               
               
                 VARIETY 
                 ‘Painted Lady’ 
               
               
                 DENOMI- 
                   
               
               
                 NATION 
                   
               
               
                 MARKET  
                 Hemp 
               
               
                 CLASS 
                   
               
               
                 PROPAGA- 
                 Typically propagated asexually via stem cuttings and  
               
               
                 TION 
                 F1 rootstock. Cloning is performed in a propagation  
               
               
                   
                 chamber with low par reduced spectrum lighting.  
               
               
                   
                 The propagation chamber has reduced environmental  
               
               
                   
                 variablity to maintain a relative humidity at or about  
               
               
                   
                 75% and a constant temperature of 72 degrees  
               
               
                   
                 Fahrenheit. 
               
               
                 PLANT  
                 Bush-like structure. This branching of this structure  
               
               
                 DESCRIP- 
                 is further broadened by removing the terminal flower.  
               
               
                 TION 
                 The natural height of the plant at annual harvest  
               
               
                   
                 (maturity) is 4-5 feet, and the width is 5.5-7 feet.  
               
               
                   
                 The plant is primarily self supporting through its  
               
               
                   
                 life cycle. 
               
               
                 STEM  
                 Hollow ribbed stem with some red and purple  
               
               
                 DESCRIP- 
                 striping on the more mature woody parts. Stem  
               
               
                 TION 
                 thickness is medium, stem depth of ribbing is  
               
               
                   
                 medium, and stem pith in cross-section is medium.  
               
               
                   
                 Stems are strong with a tendency to bend rather than  
               
               
                   
                 snap. Texture is coarse due to sessile glands covering 
               
               
                   
                 the surface. Color is wintergreen (RHS 128A)  
               
               
                   
                 changing to emerald green (RHS N134C) at maturity. 
               
               
                 BUD  
                 Early flower development and resin production in  
               
               
                 DESCRIP- 
                 flowering, comparatively short broad stigmas that are  
               
               
                 TION 
                 consistently white (RHS N155C) in color. Finished  
               
               
                   
                 buds or pistillate inflorescences range in size from  
               
               
                   
                 16-24 inches in length on the primary branches, with  
               
               
                   
                 broken internodal buds continuing down the  
               
               
                   
                 branches for 3 feet toward the base of the plant with  
               
               
                   
                 increased sparsity. Finished buds range in diameter  
               
               
                   
                 from 2-3 inches at the top of the branch down to 0.5 
               
               
                   
                 inches towards the base of the plant. Finished buds  
               
               
                   
                 range in color from light green (RHS 140C) to  
               
               
                   
                 purple (RHS N74A). 
               
               
                 LEAF  
                 Each leaf is compound and contains either 7 or 9  
               
               
                 DESCRIP- 
                 leaflets with uniform and mild serration. The leaflets  
               
               
                 TION 
                 are narrow with moderate separation at the base. The  
               
               
                   
                 average leaf length is 3 inches, the central leaflet  
               
               
                   
                 average length is 3.5-4 inches, and the central leaflet  
               
               
                   
                 average width is ⅝ of an inch The leaf surface  
               
               
                   
                 texture is rough on the top due to stiff hairs and both  
               
               
                   
                 sides are covered with sessile glands. The leaf  
               
               
                   
                 fragrance is lavender with fruity mango notes. The  
               
               
                   
                 upper leaf surface color is strong yellowish green  
               
               
                   
                 (RHS 134B) and the lower leaf surface color is  
               
               
                   
                 strong yellow green (RHS 142A). The venation  
               
               
                   
                 pattern is primarily palmate, serrated leaflets with  
               
               
                   
                 no double serration only a single vein to each  
               
               
                   
                 serrated tip. The leaf has unique exaggerated  
               
               
                   
                 serrations. 
               
               
                   
                 The petiole has an average length of 2 inches and  
               
               
                   
                 an average diameter of 4 mm. The petiole trichome  
               
               
                   
                 type is a sessile gland with limited to no capitate  
               
               
                   
                 head. The petiole intensity of anthocyanin is weak,  
               
               
                   
                 and the petiole color is brilliant yellowish green  
               
               
                   
                 (RHS 134C). The stipule is 1/16-⅛ of an inch in  
               
               
                   
                 size, and narrow and needle-like in shape. The stipule  
               
               
                   
                 contains no visible trichomes. The stipule color is  
               
               
                   
                 brilliant yellow green (RHS 142B). 
               
               
                 FLOWER  
                 The primary flowers when finished are large at 2.5-3 
               
               
                 DESCRIP- 
                 inches in diameter and 16-24 inches long on average,  
               
               
                 TION 
                 very dense or tight in structure, covered densely in  
               
               
                   
                 trichomes. The flowers have a high calyx to leaf  
               
               
                   
                 ratio which provides stronger cannabinoid production.  
               
               
                   
                 The finished flower is highly resinous and aromatic.  
               
               
                   
                 The appearance of the dried flower is sparkling with  
               
               
                   
                 trichomes. The leafy parts of the flower (primarily  
               
               
                   
                 sugar leaves) retain green as a base color mixed with  
               
               
                   
                 red, purple, and blue hues. The pistillate inflorescence  
               
               
                   
                 is moderate purplish red (RHS 186B) and the stigma  
               
               
                   
                 remains white until maturity. The immature stigma  
               
               
                   
                 color is light yellow green (RHS 144D), and the  
               
               
                   
                 mature stigma color is dark greenish yellow (RHS  
               
               
                   
                 152C). The average length of the stigma is 4 mm.  
               
               
                   
                 The flowering season is May through October. The  
               
               
                   
                 average bract size is 1-1.5 cm, and the bract quantity  
               
               
                   
                 per flower is several dozen (the flower size can vary  
               
               
                   
                 significantly depending upon the flower location on  
               
               
                   
                 the plant as well as the overall size of the plant).  
               
               
                   
                 The bract shape is oval, and the bract trichome type  
               
               
                   
                 is sessile. The bract color is strong yellowish green  
               
               
                   
                 (RHS 141C). The average bracteole size is 2-3 mm,  
               
               
                   
                 and the bracteole quantity per flower is 4-6. The  
               
               
                   
                 bracteole has a sessile trichome type. The bracteole  
               
               
                   
                 shape is arrow-like, and the bracteole color is  
               
               
                   
                 brilliant green (RHS 128A). The average sugar leaf  
               
               
                   
                 size is 10 cm. The sugar leaf has both glandular and  
               
               
                   
                 non-glandular trichome types. The sugar leaf color  
               
               
                   
                 is strong yellowish green (RHS 141C). 
               
               
                 FRUIT  
                 The fruit type is pistillate inflorescence. The fruit  
               
               
                 DESCRIP- 
                 shape is a tall and fat pinecone shape. The average  
               
               
                 TION 
                 size of the fruit is 4 inches in diameter and 7 inches  
               
               
                   
                 in length. The fruit color is deep purplish red (RHS  
               
               
                   
                 187D). 
               
               
                 ROOT  
                 Short taproot from seed that is generally not deeper  
               
               
                 DESCRIP- 
                 than 30 cm at maturity. There are dense subsurface 
               
               
                 TION 
                 lateral roots spreading out to 70 cm from the base 
               
               
                   
                 of the plant. 
               
               
                 SEED  
                 The seeds are 2.5-3 mm long with a slightly oval  
               
               
                 DESCRIP- 
                 shape that has a depression at one end and a rounded 
               
               
                 TION 
                 point at the opposite end. The seeds are dark purple 
               
               
                   
                 to black in color. With veins throughout the leaf 
               
               
                   
                 surface when closely observed. The seed color of 
               
               
                   
                 testa is greyish yellow green (RHS 198A), and seed 
               
               
                   
                 marbling is medium. The average weight per 1000 
               
               
                   
                 seeds is low (less than 10 grams). 
               
               
                 FRAGRANCE/ 
                 Sharp astringent tropical fruit odor with notes of  
               
               
                 TASTE 
                 blueberries and anise in the ripe flower/bud. The 
               
               
                   
                 stem also produces a sour aroma with notes of 
               
               
                   
                 blueberry when rubbed. 
               
               
                 GROWTH  
                 Annual, dioecious, erect herb. Unique internodal  
               
               
                 HABIT 
                 spacing consisting of two nodes 1 inch apart  
               
               
                   
                 separated by a 2.5-3 inch gap then followed again 
               
               
                   
                 by 2 nodes separated by 1 inch. 
               
               
                 DISEASE  
                 Naturally pest and disease resistant. Bred from pest,  
               
               
                 RESISTANCE 
                 pathogen, and virus free mother stock to reduce the  
               
               
                   
                 potential for any predisposition for a virus or 
               
               
                   
                 pathogen to influence the genetic material. 
               
               
                   
                 The plant has shown resistance to the pests fall  
               
               
                   
                 armyworm ( Spodoptera ornithogalh ), two spotted 
               
               
                   
                 spider mite ( Tetranychus urticae ), and broad mite 
               
               
                   
                 ( Polyphagotarsonemus latus ). The plant is resistant 
               
               
                   
                 to most common molds, including Botrytis cinerea, 
               
               
                   
                 and powdery mildews, such as  Podosphaera xanthii . 
               
               
                   
                 In addition, the plant has shown some resistance to 
               
               
                   
                 Hyphomycetes such as  Fusarium  spp. 
               
               
                 PRODUC- 
                 The plant is grown annually in a moderate climate  
               
               
                 TIVITY 
                 with a growing season of 5 to 6 months. Over an 
               
               
                   
                 average growing summer, the plant will yield 1.5-2.5 
               
               
                   
                 lbs. of dried plant material. Average time from 
               
               
                   
                 planting to harvest is 4.5-5.5 months. The shipping 
               
               
                   
                 quality is high. The market use is for oil production 
               
               
                   
                 and smokable flower production. The storage life is 
               
               
                   
                 3-6 months depending on storage climate. 
               
               
                 TEMPER- 
                 Frost resistance down to 28° Fahrenheit as a young  
               
               
                 ATURE 
                 sapling. Frost resistance down to 22° Fahrenheit as  
               
               
                 TOLERANCE 
                 a mature flowering plant 
               
               
                 GROWTH  
                 Outdoor: Annual from March to October. Indoor:  
               
               
                 CYCLE 
                 Photoperiod dependent vegetative cycle with an 
               
               
                   
                 approximate 50 day flowering cycle. 
               
               
                   
               
            
           
         
       
     
     COMPARISON TO RELATED KNOWN CULTIVAR 
     ‘Painted Lady’ is distinguished from ‘Eighty Eight’ (U.S. Plant patent application Ser. No. 16/602,563) in that the plant of ‘Painted Lady’ is slightly taller in height than the plant of ‘Eighty Eight’. Further, the flowers of ‘Painted Lady’ have internode spacing that is more spread out, which provides the flowers with a longer appearance than the flowers of ‘Eighty Eight’. In addition, the overall color palette of ‘Painted Lady’ is lighter than the overall color palette of ‘Eighty Eight’.