Patent Publication Number: US-2023134869-A1

Title: Crystal form of free alkali of nitrogen-containing aromatic derivatives

Description:
FIELD OF THE INVENTION 
     The present invention belongs to the field of biomedicine, and in particular relates to a crystal form of free base of nitrogen-containing heteroaromatic derivative, a method for preparing the same, and a use thereof. 
     BACKGROUND OF THE INVENTION 
     Src homology-2 domain-containing phosphatase 2 (SHP-2), also known as tyrosine-protein phosphatase non-receptor type 11 (PTPN11), is encoded by PTPN11 gene, and belongs to the protein tyrosine phosphatase (PTP) family. As a downstream signal molecule of cytokines, growth factors and other extracellular stimulating factors, SHP-2 is widely expressed in various tissues and cells of the body, participates in cell signal transduction, and regulates cell growth, differentiation, migration, metabolism, gene transcription, immune response and the like. 
     SHP-2 has three main structural parts: SH-2 domain (N-SH2 and C-SH2), PTP active domain, and C-terminal (having a tyrosine phosphorylation site). The SH2 domain is highly conserved, which is a phosphotyrosine binding site and mediates the binding of the PTP domain to its ligand. 
     SHP-2 has two main states in vivo: inactivated state and activated state. In the inactivated state, N-SH2 in SHP-2 binds to the PTP domain, because the PTP domain is occupied, SHP-2 is inactivated. When N-SH2 specifically binds to the phosphorylated tyrosine residue ligand, the PTP domain is re-exposed and SHP-2 resumes its activity. Latest studies show that SHP-2 can also form dimers in vivo, which can also lead to SHP-2 inactivation. 
     SHP-2 mainly functions by regulating signal pathways such as ERK/MAPK, JAK-STAT, PI3K/AKT, Hippo, Wnt/β-catenin, so as to maintain biological development and homeostasis. Specific studies show that SHP-2 participates in the activation of the ERK/MAPK pathway by directly binding to receptor tyrosine kinase (RTK) or scaffold. In addition, activated SHP-2 can also recruit GRB2/SOS, and indirectly promote the activation of the RAS signaling pathway. Moreover, SHP-2 is also involved in signal transduction that inhibits immune response. For example, SHP-2 and SHP-1 can bind to and activate immunosuppressive receptors (such as PD-1), and block T cell activation. 
     As an important cell signaling factor, SHP-2 mutations are closely related to many diseases. Studies show that SHP-2 mutations are found in neuroblastoma, acute myeloid leukemia (AML, 4%), breast cancer, non-small cell lung cancer (NSCLC, 10%), lung adenocarcinoma (30%), esophageal cancer, head and neck tumor, melanoma and gastric cancer. 
     The mutation sites of SHP-2 mostly occur in N-SH2 and PTP active regions. The mutations reduce the mutual inhibition of N-CH2/PTP domains, and lead to highly active SHP-2, for example, Cys459Ser mutant, E76K mutant and the like will affect the activity of SHP-2. Studies show that highly active SHP-2 is closely related to inflammation, liver cirrhosis, the toxin CagA secreted by  Helicobacter pylori  and the like. Highly active SHP-2 can lead to tumor regeneration and development, and is equivalent to a proto-oncogene. With the deepening of the understanding of SHP-2, SHP-2 has been used as a tumor treatment target for drug development. 
     At present, several SHP-2 allosteric inhibitors have entered the clinical research phase. For example, TNO-155 developed by Novartis, RMC-4630 developed by Revolution Medicine, and JAB-3068 developed by Beijing Jacobio have all entered the phase I clinical research phase. However, there is no SHP-2 inhibitor on the market for the treatment of Noonan syndrome, leopard syndrome, leukemia, neuroblastoma, melanoma, breast cancer, esophageal cancer, head and neck tumor, lung cancer and colon cancer. Therefore, there is an urgent need to develop a class of SHP-2 inhibitor drugs with good pharmaceutical properties. 
     The PCT patent application (application number: PCT/CN2019/110314) discloses the structure of a series of nitrogen-containing heteroaromatic derivative inhibitor. In the subsequent research and development, in order to obtain a product that can be readily processed, filtered and dried and to achieve features such as convenient storage, long-term stability and high bioavailability, the present invention has conducted a comprehensive study on the free base of the above substances, and is dedicated to obtaining the most suitable crystal forms. 
     SUMMARY OF THE INVENTION 
     All contents involved in the patent application PCT/CN2019/110314 are incorporated into the present invention by way of reference. 
     The objective of the present invention is to provide a crystal form of a compound of formula (I), the structure of which is shown in formula (I): 
     
       
         
         
             
             
         
       
     
     wherein: 
     R 1  is selected from the group consisting of hydrogen, halogen, C 1-6  alkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl, wherein the C 1-6  alkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen, amino, nitro, hydroxy, cyano, oxo, C 1-6  alkyl, C 1-6  deuterated alkyl, C 1-6  haloalkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 1-6  haloalkoxy, C 2-6  alkenyl, C 2-6  alkynyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl; 
     R 2  is selected from the group consisting of hydrogen, amino and C 1-6  alkyl; 
     R 3  is selected from the group consisting of hydrogen, halogen, amino, cyano, C 1-6  alkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 3-8  cycloalkyl and 3 to 12 membered heterocyclyl, wherein the C 1-6  alkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 3-8  cycloalkyl and 3 to 12 membered heterocyclyl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen, amino, nitro, hydroxy, cyano, oxo, C 1-6  alkyl, C 1-6  deuterated alkyl, C 1-6  haloalkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 1-6  haloalkoxy, C 2-6  alkenyl, C 2-6  alkynyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl; 
     R 4  is selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, C 1-6  alkyl, C 1-6  deuterated alkyl, C 1-6  haloalkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 1-6  haloalkoxy, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl; 
     R 5  and R 6  are each independently selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, C 1-6  alkyl, C 1-6  deuterated alkyl, C 1-6  haloalkyl, C 1-6  alkoxy, C 1-6  haloalkoxy, C 1-6  hydroxyalkyl, C 2-6  alkenyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl, wherein the C 1-6  alkyl, C 1-6  deuterated alkyl, C 1-6  haloalkyl, C 1-6  alkoxy, C 1-6  haloalkoxy, C 1-6  hydroxyalkyl, C 2-6  alkenyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen, amino, nitro, hydroxy, cyano, oxo, C 1-6  alkyl, C 1-6  deuterated alkyl, C 1-6  haloalkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 1-6  haloalkoxy, C 2-6  alkenyl, C 2-6  alkynyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl; 
     or, R 5  and R 6  are bonded to form a C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl or 5 to 12 membered heteroaryl, wherein the C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl are each optionally further substituted by one or more substituent(s) selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, oxo, C 1-6  alkyl, C 1-6  deuterated alkyl, C 1-6  haloalkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 1-6  haloalkoxy, C 2-6  alkenyl, C 2-6  alkynyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl. 
     In a further preferred embodiment of the present invention, R 1  is selected from the group consisting of hydrogen, halogen, C 1-6  alkyl, C 1-6  hydroxyalkyl, C 3-8  cycloalkyl and 3 to 12 membered heterocyclyl, wherein the C 1-6  alkyl, C 1-6  hydroxyalkyl, C 3-8  cycloalkyl and 3 to 12 membered heterocyclyl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen, hydroxy, oxo, C 1-6  alkyl and C 3-8  cycloalkyl; more preferably selected from the group consisting of hydrogen, halogen, C 1-3  alkyl, C 1-3  hydroxyalkyl, C 3-6  cycloalkyl and 3 to 6 membered heterocyclyl, wherein the C 1-3  alkyl, C 1-3  hydroxyalkyl, C 3-6  cycloalkyl and 3 to 6 membered heterocyclyl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen, hydroxy, oxo, C 1-3  alkyl and C 3-6  cycloalkyl; and further preferably selected from the group consisting of hydrogen, fluorine, bromine, methyl, hydroxyethyl, 
     
       
         
         
             
             
         
       
     
     In a further preferred embodiment of the present invention, R 2  is selected from the group consisting of hydrogen, amino and C 1-6  alkyl; preferably selected from the group consisting of hydrogen, amino and C 1-3  alkyl; more preferably selected from the group consisting of hydrogen, amino, methyl, ethyl and propyl; and further preferably selected from the group consisting of hydrogen, amino and methyl. 
     In a further preferred embodiment of the present invention, R 3  is selected from the group consisting of hydrogen, halogen, amino, C 1-6  alkyl, C 3-8  cycloalkyl and 3 to 12 membered heterocyclyl, wherein the C 1-6  alkyl, C 3-8  cycloalkyl and 3 to 12 membered heterocyclyl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen and oxo; preferably selected from the group consisting of hydrogen, halogen, amino, C 1-3  alkyl, C 3-6  cycloalkyl and 3 to 6 membered heterocyclyl, wherein the C 1-3  alkyl, C 3-6  cycloalkyl and 3 to 6 membered heterocyclyl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen and oxo; and further preferably selected from the group consisting of amino, chlorine, cyclopropyl, azacyclobutyl, tetrahydropyrrolyl, morpholinyl, 
     
       
         
         
             
             
         
       
     
     In a further preferred embodiment of the present invention, R 4  is selected from the group consisting of hydrogen, halogen, C 1-6  alkyl and C 3-8  cycloalkyl; preferably selected from the group consisting of hydrogen, halogen, C 1-3  alkyl and C 3-6  cycloalkyl; and further preferably selected from the group consisting of hydrogen, fluorine, chlorine, methyl and cyclopropyl. 
     In a further preferred embodiment of the present invention, R 5  and R 6  are each independently selected from the group consisting of hydrogen, C 1-6  alkyl, C 1-6  haloalkyl, C 2-6  alkenyl and C 3-8  cycloalkyl, wherein the C 1-6  alkyl, C 1-6  haloalkyl, C 2-6  alkenyl and C 3-8  cycloalkyl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen and C 1-6  alkyl; preferably selected from the group consisting of hydrogen, C 1-3  alkyl, C 2-4  alkenyl and C 3-6  cycloalkyl, wherein the C 1-3  alkyl, C 2-4  alkenyl and C 3-6  cycloalkyl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen and C 1-3  alkyl; and further preferably selected from the group consisting of hydrogen, methyl, ethyl, isopropyl, vinyl, cyclopropyl, 
     
       
         
         
             
             
         
       
     
     or, R 5  and R 6  are bonded to form a C 3-8  cycloalkyl, C 6-12  aryl or 5 to 12 membered heteroaryl, wherein the C 3-8  cycloalkyl, C 6-12  aryl and 5 to 12 membered heteroaryl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen and C 1-6  alkyl; preferably form a C 3-6  cycloalkyl, phenyl or 5 to 6 membered heteroaryl, wherein the C 3-6  cycloalkyl, phenyl and 5 to 6 membered heteroaryl are each optionally further substituted by one or more substituent(s) selected from the group consisting of deuterium, halogen and C 1-3  alkyl; and further preferably form 
     
       
         
         
             
             
         
       
     
     In a further preferred embodiment of the present invention, the structure of the compound is as shown in formula (II): 
     
       
         
         
             
             
         
       
     
     wherein: 
     ring A is selected from the group consisting of C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-10  aryl and 5 to 12 membered heteroaryl; 
     R a  is selected from the group consisting of hydrogen, deuterium, halogen, amino, nitro, hydroxy, cyano, C 1-6  alkyl, C 1-6  deuterated alkyl, C 1-6  haloalkyl, C 1-6  alkoxy, C 1-6  hydroxyalkyl, C 1-6  haloalkoxy, C 2-6  alkenyl, C 2-6  alkynyl, C 3-8  cycloalkyl, 3 to 12 membered heterocyclyl, C 6-12  aryl and 5 to 12 membered heteroaryl; and 
     x is 0, 1, 2 or 3. 
     In a further preferred embodiment of the present invention, ring A is phenyl. 
     The present invention also provides a method for preparing a crystal form of the compound of formula (I), specifically comprising the following steps of: 
     1) weighing an appropriate amount of free base, and suspending it in a poor solvent to obtain a suspension; the suspension density is preferably 50 to 200 mg/mL; 
     2) shaking the suspension obtained in step 1) at 0 to 40° C. for 1 to 10 days; 
     3) rapidly centrifuging the suspension obtained in step 2), removing the supernatant, and drying the remaining solid in a vacuum drying oven at 40° C. to constant weight to obtain the target product; 
     wherein: 
     the poor solvent in step 1) is selected from the group consisting of acetone, ethyl acetate, acetonitrile, ethanol, 88% acetone, tetrahydrofuran, dichloromethane, 1,4-dioxane, benzene, toluene, isopropanol, n-butanol, isobutanol, N,N-dimethylformamide, N,N-dimethylacetamide, n-propanol, tert-butanol, 2-butanone and 3-pentanone; and preferably selected from the group consisting of 3-pentanone, acetonitrile, dichloromethane and 1,4-dioxane. 
     The present invention also provides a method for preparing a crystal form of the compound of formula (I), specifically comprising the following steps of: 
     1) weighing an appropriate amount of free base, and dissolving it in a good solvent; 
     2) adding an anti-solvent to the solution obtained in step 1) at 0 to 25° C., and stirring the solution until a solid is precipitated; 
     3) rapidly centrifuging the suspension obtained in step 2), removing the supernatant, and drying the remaining solid in a vacuum drying oven at 40° C. to constant weight to obtain the target product; 
     wherein: 
     the good solvent in step 1) is selected from the group consisting of methanol, acetone, ethyl acetate, acetonitrile, ethanol, 8800 acetone, tetrahydrofuran, dichloromethane, 1,4-dioxane, benzene, toluene, isopropanol, n-butanol, isobutanol, N,N-dimethylformamide, N,N-dimethylacetamide, n-propanol, tert-butanol, 2-butanone and 3-pentanone; and preferably selected from the group consisting of N,N-dimethylformamide and acetonitrile. 
     The anti-solvent in step 2) is selected from the group consisting of heptane, water, methyl tert-butyl ether, toluene and isopropyl ether; and preferably water. 
     In a further preferred embodiment of the present invention, the crystal form is crystal form A of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the X-ray powder diffraction pattern thereof has diffraction peaks at 2θ of 9.0°, 15.2°, 20.2° and 23.0°; further has diffraction peaks at 2θ of 0.7°, 12.3°, 15.4°, 19.8°, 23.5° and 27.1°; and more further has diffraction peaks at 2θ of 4.5°, 13.7°, 14.6°, 16.8°, 18.1°, 21.5°, 27.7° and 28.2°. 
     Using Cu—Kα radiation, the characteristic X-ray diffraction peaks represented by 2θ angle and interplanar spacing d value are shown in Table 1. 
     
       
         
           
               
               
             
               
                   
                 TABLE 1 
               
             
            
               
                   
                   
               
               
                   
                 XRPD diffraction data of crystal form A 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 2θ 
                 d 
                 Peak 
                 Proportion 
                   
                 Proportion 
               
               
                 No. 
                 (±0.2°) 
                 value 
                 height 
                 (I %) 
                 Area 
                 (I %) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 4.46 
                 19.7967 
                 336 
                 59.9 
                 2777 
                 29.9 
               
               
                 2 
                 8.95 
                 9.8727 
                 553 
                 98.6 
                 6611 
                 71.3 
               
               
                 3 
                 9.677 
                 9.1323 
                 292 
                 52 
                 2672 
                 28.8 
               
               
                 4 
                 12.348 
                 7.1619 
                 257 
                 45.8 
                 3318 
                 35.8 
               
               
                 5 
                 13.431 
                 6.587 
                 90 
                 16 
                 2814 
                 30.3 
               
               
                 6 
                 13.732 
                 6.4435 
                 131 
                 23.4 
                 2807 
                 30.3 
               
               
                 7 
                 14.58 
                 6.0703 
                 129 
                 23 
                 2026 
                 21.8 
               
               
                 8 
                 15.213 
                 5.8193 
                 561 
                 100 
                 9274 
                 100 
               
               
                 9 
                 15.435 
                 5.7362 
                 364 
                 64.9 
                 4743 
                 51.1 
               
               
                 10 
                 16.75 
                 5.2884 
                 194 
                 34.6 
                 3530 
                 38.1 
               
               
                 11 
                 17.072 
                 5.1895 
                 81 
                 14.4 
                 1497 
                 16.1 
               
               
                 12 
                 18.129 
                 4.8892 
                 90 
                 16 
                 1303 
                 14.1 
               
               
                 13 
                 19.838 
                 4.4718 
                 234 
                 41.7 
                 5413 
                 58.4 
               
               
                 14 
                 20.199 
                 4.3926 
                 337 
                 60.1 
                 5137 
                 55.4 
               
               
                 15 
                 21.454 
                 4.1383 
                 106 
                 18.9 
                 1754 
                 18.9 
               
               
                 16 
                 22.974 
                 3.868 
                 537 
                 95.7 
                 8148 
                 87.9 
               
               
                 17 
                 23.463 
                 3.7884 
                 335 
                 59.7 
                 6702 
                 72.3 
               
               
                 18 
                 27.05 
                 3.2936 
                 183 
                 32.6 
                 2983 
                 32.2 
               
               
                 19 
                 27.741 
                 3.2132 
                 175 
                 31.2 
                 3116 
                 33.6 
               
               
                 20 
                 28.169 
                 3.1653 
                 93 
                 16.6 
                 1534 
                 16.5 
               
               
                   
               
            
           
         
       
     
     The crystal form of the present invention is crystal form A of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the DSC spectrum thereof is substantially as shown in  FIG.  1   ; and the TGA spectrum thereof is substantially as shown in  FIG.  2   . 
     In a further preferred embodiment of the present invention, the crystal form is crystal form B of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the X-ray powder diffraction pattern thereof has diffraction peaks at 2θ of 7.9°, 14.6° and 16.2°; also has diffraction peaks at 2θ of 18.1°, 19.2°, 20.2°, 24.7° and 26.8°; further has diffraction peaks at 2θ of 14.9°, 27.2°, 28.1° and 30.5°; more further has diffraction peaks at 2θ of 13.9°, 15.6°, 16.9° and 19.8°; and most preferably, the XRPD pattern thereof is substantially as shown in  FIG.  3   . 
     Using Cu—Kα radiation, the characteristic X-ray diffraction peaks represented by 2θ angle and interplanar spacing d value are shown in Table 2. 
     
       
         
           
               
               
             
               
                   
                 TABLE 2 
               
             
            
               
                   
                   
               
               
                   
                 XRPD diffraction data of crystal form B 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 2θ 
                 d 
                 Peak 
                 Proportion 
                   
                 Proportion 
               
               
                 No. 
                 (±0.2°) 
                 value 
                 height 
                 (I %) 
                 Area 
                 (I %) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 6.31 
                 13.9956 
                 80 
                 8 
                 396 
                 2.5 
               
               
                 2 
                 7.853 
                 11.2482 
                 690 
                 68.7 
                 5922 
                 37.1 
               
               
                 3 
                 9.923 
                 8.9068 
                 61 
                 6.1 
                 834 
                 5.2 
               
               
                 4 
                 13.911 
                 6.3607 
                 221 
                 22 
                 1254 
                 7.9 
               
               
                 5 
                 14.564 
                 6.0771 
                 733 
                 72.9 
                 9648 
                 60.5 
               
               
                 6 
                 14.925 
                 5.9309 
                 547 
                 54.4 
                 6387 
                 40 
               
               
                 7 
                 15.637 
                 5.6625 
                 105 
                 10.4 
                 1085 
                 6.8 
               
               
                 8 
                 16.166 
                 5.4781 
                 1005 
                 100 
                 13053 
                 81.8 
               
               
                 9 
                 16.92 
                 5.2359 
                 139 
                 13.8 
                 1536 
                 9.6 
               
               
                 10 
                 18.11 
                 4.8942 
                 948 
                 94.3 
                 14765 
                 92.6 
               
               
                 11 
                 19.183 
                 4.6229 
                 707 
                 70.3 
                 6787 
                 42.5 
               
               
                 12 
                 19.831 
                 4.4733 
                 317 
                 31.5 
                 5379 
                 33.7 
               
               
                 13 
                 20.219 
                 4.3883 
                 838 
                 83.4 
                 14241 
                 89.3 
               
               
                 14 
                 21.899 
                 4.0553 
                 126 
                 12.5 
                 1340 
                 8.4 
               
               
                 15 
                 23.135 
                 3.8413 
                 126 
                 12.5 
                 697 
                 4.4 
               
               
                 16 
                 24.679 
                 3.6044 
                 447 
                 44.5 
                 7296 
                 45.7 
               
               
                 17 
                 26.828 
                 3.3204 
                 820 
                 81.6 
                 15952 
                 100 
               
               
                 18 
                 27.211 
                 3.2745 
                 232 
                 23.1 
                 8341 
                 52.3 
               
               
                 19 
                 27.843 
                 3.2016 
                 219 
                 21.8 
                 6157 
                 38.6 
               
               
                 20 
                 28.123 
                 3.1703 
                 265 
                 26.4 
                 6160 
                 38.6 
               
               
                 21 
                 29.136 
                 3.0624 
                 131 
                 13 
                 1588 
                 10 
               
               
                 22 
                 30.459 
                 2.9323 
                 227 
                 22.6 
                 4827 
                 30.3 
               
               
                 23 
                 31.428 
                 2.8441 
                 131 
                 13 
                 2164 
                 13.6 
               
               
                 24 
                 31.833 
                 2.8088 
                 80 
                 8 
                 470 
                 2.9 
               
               
                 25 
                 32.483 
                 2.7541 
                 67 
                 6.7 
                 851 
                 5.3 
               
               
                 26 
                 33.25 
                 2.6923 
                 78 
                 7.8 
                 709 
                 4.4 
               
               
                 27 
                 34.473 
                 2.5996 
                 55 
                 5.5 
                 1185 
                 7.4 
               
               
                 28 
                 34.915 
                 2.5676 
                 63 
                 6.3 
                 2273 
                 14.2 
               
               
                 29 
                 38.185 
                 2.3549 
                 55 
                 5.5 
                 3052 
                 19.1 
               
               
                 30 
                 38.787 
                 2.3197 
                 69 
                 6.9 
                 1824 
                 11.4 
               
               
                   
               
            
           
         
       
     
     The crystal form of the present invention is crystal form B of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the DSC spectrum thereof is substantially as shown in  FIG.  4   ; and the TGA spectrum thereof is substantially as shown in  FIG.  5   . 
     In a further preferred embodiment of the present invention, the crystal form is crystal form C of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the X-ray powder diffraction pattern thereof has diffraction peaks at 2θ of 9.4°, 14.5° and 20.3°; also has diffraction peaks at 2θ of 22.2°, 22.7°, 22.9° and 26.2°; further has diffraction peaks at 2θ of 8.2°, 14.9°, 23.8°, 27.5°, 28.1°, 29.3°, 30.2° and 31.7°; and more further has diffraction peaks at 2θ of 12.0°, 16.4°, 25.5°, 28.6°, 34.8° and 35.4°. 
     Using Cu—Kα radiation, the characteristic X-ray diffraction peaks represented by 2θ angle and interplanar spacing d value are shown in Table 3. 
     
       
         
           
               
               
             
               
                   
                 TABLE 3 
               
             
            
               
                   
                   
               
               
                   
                 XRPD diffraction data of crystal form C 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 2θ 
                 d 
                 Peak 
                 Proportion 
                   
                 Proportion 
               
               
                 No. 
                 (±0.2°) 
                 value 
                 height 
                 (I %) 
                 Area 
                 (I %) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 8.155 
                 10.8327 
                 137 
                 20.9 
                 1410 
                 12.1 
               
               
                 2 
                 9.412 
                 9.3886 
                 231 
                 35.3 
                 2719 
                 23.2 
               
               
                 3 
                 12.023 
                 7.3549 
                 108 
                 16.5 
                 578 
                 4.9 
               
               
                 4 
                 14.522 
                 6.0944 
                 654 
                 100 
                 9549 
                 81.6 
               
               
                 5 
                 14.926 
                 5.9303 
                 380 
                 58.1 
                 6492 
                 55.5 
               
               
                 6 
                 16.351 
                 5.4166 
                 181 
                 27.7 
                 1568 
                 13.4 
               
               
                 7 
                 19.076 
                 4.6485 
                 87 
                 13.3 
                 2344 
                 20 
               
               
                 8 
                 19.63 
                 4.5187 
                 77 
                 11.8 
                 1134 
                 9.7 
               
               
                 9 
                 20.32 
                 4.3666 
                 604 
                 92.4 
                 6581 
                 56.2 
               
               
                 10 
                 22.206 
                 4 
                 414 
                 63.3 
                 5017 
                 42.9 
               
               
                 11 
                 22.652 
                 3.9222 
                 384 
                 58.7 
                 11700 
                 100 
               
               
                 12 
                 22.933 
                 3.8748 
                 465 
                 71.1 
                 10729 
                 91.7 
               
               
                 13 
                 23.849 
                 3.7279 
                 277 
                 42.4 
                 3805 
                 32.5 
               
               
                 14 
                 25.493 
                 3.4912 
                 116 
                 17.7 
                 2306 
                 19.7 
               
               
                 15 
                 26.198 
                 3.3987 
                 367 
                 56.1 
                 6160 
                 52.6 
               
               
                 16 
                 27.517 
                 3.2388 
                 258 
                 39.4 
                 3216 
                 27.5 
               
               
                 17 
                 28.081 
                 3.175 
                 266 
                 40.7 
                 3946 
                 33.7 
               
               
                 18 
                 28.55 
                 3.1239 
                 143 
                 21.9 
                 2439 
                 20.8 
               
               
                 19 
                 29.341 
                 3.0415 
                 149 
                 22.8 
                 1702 
                 14.5 
               
               
                 20 
                 30.153 
                 2.9614 
                 207 
                 31.7 
                 3160 
                 27 
               
               
                 21 
                 31.673 
                 2.8226 
                 178 
                 27.2 
                 3637 
                 31.1 
               
               
                 22 
                 33.013 
                 2.7111 
                 61 
                 9.3 
                 1617 
                 13.8 
               
               
                 23 
                 34.816 
                 2.5747 
                 119 
                 18.2 
                 1530 
                 13.1 
               
               
                 24 
                 35.384 
                 2.5346 
                 112 
                 17.1 
                 2291 
                 19.6 
               
               
                   
               
            
           
         
       
     
     The crystal form of the present invention is crystal form C of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the DSC spectrum thereof is substantially as shown in  FIG.  6   ; and the TGA spectrum thereof is substantially as shown in  FIG.  7   . 
     In a further preferred embodiment of the present invention, the crystal form is crystal form D of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the X-ray powder diffraction pattern thereof has diffraction peaks at 2θ of 5.1°, 13.4° and 18.3°; also has diffraction peaks at 2θ of 18.0°, 19.9°, 20.7° and 22.6°; further has diffraction peaks at 2θ of 13.7°, 16.8°, 17.7°, 19.0°, 22.3° and 27.5°; and more further has diffraction peaks at 2θ of 16.1°, 22.9°, 24.9°, 25.5° and 28.9°. 
     Using Cu—Kα radiation, the characteristic X-ray diffraction peaks represented by 2θ angle and interplanar spacing d value are shown in Table 4. 
     
       
         
           
               
               
             
               
                   
                 TABLE 4 
               
             
            
               
                   
                   
               
               
                   
                 XRPD diffraction data of crystal form D 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 2θ 
                 d 
                 Peak 
                 Proportion 
                   
                 Proportion 
               
               
                 No. 
                 (±0.2°) 
                 value 
                 height 
                 (I %) 
                 Area 
                 (I %) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 5.077 
                 17.3927 
                 634 
                 100 
                 4794 
                 30.6 
               
               
                 2 
                 13.369 
                 6.6176 
                 377 
                 59.5 
                 4314 
                 27.5 
               
               
                 3 
                 13.652 
                 6.481 
                 250 
                 39.4 
                 2141 
                 13.7 
               
               
                 4 
                 16.137 
                 5.4881 
                 107 
                 16.9 
                 914 
                 5.8 
               
               
                 5 
                 16.756 
                 5.2866 
                 300 
                 47.3 
                 2486 
                 15.9 
               
               
                 6 
                 17.728 
                 4.999 
                 206 
                 32.5 
                 5075 
                 32.4 
               
               
                 7 
                 17.928 
                 4.9437 
                 437 
                 68.9 
                 8117 
                 51.8 
               
               
                 8 
                 18.251 
                 4.8567 
                 608 
                 95.9 
                 8751 
                 55.8 
               
               
                 9 
                 19.025 
                 4.6608 
                 214 
                 33.8 
                 1681 
                 10.7 
               
               
                 10 
                 19.855 
                 4.468 
                 346 
                 54.6 
                 4664 
                 29.8 
               
               
                 11 
                 20.704 
                 4.2866 
                 434 
                 68.5 
                 5726 
                 36.5 
               
               
                 12 
                 22.285 
                 3.9859 
                 372 
                 58.7 
                 12254 
                 78.2 
               
               
                 13 
                 22.609 
                 3.9295 
                 467 
                 73.7 
                 15673 
                 100 
               
               
                 14 
                 22.893 
                 3.8815 
                 196 
                 30.9 
                 3486 
                 22.2 
               
               
                 15 
                 24.92 
                 3.57 
                 111 
                 17.5 
                 2065 
                 13.2 
               
               
                 16 
                 25.468 
                 3.4946 
                 128 
                 20.2 
                 2543 
                 16.2 
               
               
                 17 
                 27.517 
                 3.2388 
                 199 
                 31.4 
                 4405 
                 28.1 
               
               
                 18 
                 28.486 
                 3.1308 
                 74 
                 11.7 
                 1554 
                 9.9 
               
               
                 19 
                 28.898 
                 3.0871 
                 170 
                 26.8 
                 3959 
                 25.3 
               
               
                 20 
                 32.646 
                 2.7407 
                 77 
                 12.1 
                 684 
                 4.4 
               
               
                 21 
                 33.941 
                 2.6391 
                 74 
                 11.7 
                 1278 
                 8.2 
               
               
                   
               
            
           
         
       
     
     The crystal form of the present invention is crystal form D of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine the DSC spectrum thereof is substantially as shown in  FIG.  8   ; and the TGA spectrum thereof is substantially as shown in  FIG.  9   . 
     In a further preferred embodiment of the present invention, the crystal form is crystal form E of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the X-ray powder diffraction pattern thereof has diffraction peaks at 2θ of 8.4°, 15.3° and 18.3°; also has diffraction peaks at 2θ of 20.7°, 22.5°, 26.7° and 27.6°; further has diffraction peaks at 2θ of 13.5°, 14.6°, 15.8°, 16.0°, 16.9°, 19.2°, 20.4°, 24.0°, 24.4°, 25.5°, 28.2°, 28.9°, 31.8° and 32.2°; and more further has diffraction peaks at 2θ of 5.2°, 9.5°, 10.4°, 17.2°, 17.7°, 19.5°, 20.0°, 26.3θ°, 28.7θ°, 30.5θ°, 31.0° and 34.1°. 
     Using Cu—Kα radiation, the characteristic X-ray diffraction peaks represented by 2θ angle and interplanar spacing d value are shown in Table 5. 
     
       
         
           
               
               
             
               
                   
                 TABLE 5 
               
             
            
               
                   
                   
               
               
                   
                 XRPD diffraction data of crystal form E 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                 2θ 
                 d 
                 Peak 
                 Proportion 
                   
                 Proportion 
               
               
                 No. 
                 (±0.2°) 
                 value 
                 height 
                 (I %) 
                 Area 
                 (I %) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 1 
                 5.213 
                 16.9394 
                 142 
                 14.1 
                 1046 
                 7.9 
               
               
                 2 
                 8.399 
                 10.5189 
                 557 
                 55.4 
                 6484 
                 48.7 
               
               
                 3 
                 9.536 
                 9.2669 
                 131 
                 13 
                 1025 
                 7.7 
               
               
                 4 
                 10.431 
                 8.4736 
                 119 
                 11.8 
                 1062 
                 8 
               
               
                 5 
                 13.525 
                 6.5415 
                 203 
                 20.2 
                 1975 
                 14.8 
               
               
                 6 
                 14.586 
                 6.0678 
                 280 
                 27.9 
                 2862 
                 21.5 
               
               
                 7 
                 15.333 
                 5.7739 
                 1005 
                 100 
                 8117 
                 60.9 
               
               
                 8 
                 15.756 
                 5.6198 
                 369 
                 36.7 
                 5128 
                 38.5 
               
               
                 9 
                 16.022 
                 5.5271 
                 309 
                 30.7 
                 4761 
                 35.7 
               
               
                 10 
                 16.873 
                 5.2504 
                 284 
                 28.3 
                 2124 
                 15.9 
               
               
                 11 
                 17.158 
                 5.1637 
                 138 
                 13.7 
                 1260 
                 9.5 
               
               
                 12 
                 17.724 
                 5.0002 
                 180 
                 17.9 
                 2794 
                 21 
               
               
                 13 
                 18.331 
                 4.8358 
                 959 
                 95.4 
                 11686 
                 87.7 
               
               
                 14 
                 19.183 
                 4.623 
                 393 
                 39.1 
                 3758 
                 28.2 
               
               
                 15 
                 19.527 
                 4.5423 
                 139 
                 13.8 
                 1435 
                 10.8 
               
               
                 16 
                 20.055 
                 4.4239 
                 250 
                 24.9 
                 3421 
                 25.7 
               
               
                 17 
                 20.463 
                 4.3364 
                 316 
                 31.4 
                 8881 
                 66.7 
               
               
                 18 
                 20.745 
                 4.2781 
                 755 
                 75.1 
                 8937 
                 67.1 
               
               
                 19 
                 22.528 
                 3.9435 
                 461 
                 45.9 
                 4722 
                 35.4 
               
               
                 20 
                 23.927 
                 3.716 
                 304 
                 30.2 
                 6493 
                 48.7 
               
               
                 21 
                 24.438 
                 3.6394 
                 328 
                 32.6 
                 3799 
                 28.5 
               
               
                 22 
                 25.471 
                 3.4942 
                 227 
                 22.6 
                 2255 
                 16.9 
               
               
                 23 
                 26.28 
                 3.3883 
                 113 
                 11.2 
                 589 
                 4.4 
               
               
                 24 
                 26.667 
                 3.3401 
                 472 
                 47 
                 4830 
                 36.3 
               
               
                 25 
                 27.558 
                 3.234 
                 624 
                 62.1 
                 13324 
                 100 
               
               
                 26 
                 28.206 
                 3.1612 
                 187 
                 18.6 
                 1824 
                 13.7 
               
               
                 27 
                 28.671 
                 3.111 
                 154 
                 15.3 
                 1821 
                 13.7 
               
               
                 28 
                 28.939 
                 3.0828 
                 289 
                 28.8 
                 3092 
                 23.2 
               
               
                 29 
                 30.498 
                 2.9287 
                 175 
                 17.4 
                 1729 
                 13 
               
               
                 30 
                 31.023 
                 2.8803 
                 157 
                 15.6 
                 1960 
                 14.7 
               
               
                 31 
                 31.756 
                 2.8154 
                 168 
                 16.7 
                 5043 
                 37.8 
               
               
                 32 
                 32.221 
                 2.7759 
                 228 
                 22.7 
                 3442 
                 25.8 
               
               
                 33 
                 34.124 
                 2.6253 
                 151 
                 15 
                 1585 
                 11.9 
               
               
                 34 
                 36.496 
                 2.4599 
                 77 
                 7.7 
                 1877 
                 14.1 
               
               
                 35 
                 38.162 
                 2.3563 
                 71 
                 7.1 
                 1082 
                 8.1 
               
               
                   
               
            
           
         
       
     
     The crystal form of the present invention is crystal form E of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, the DSC spectrum thereof is substantially as shown in  FIG.  10   ; and the TGA spectrum thereof is substantially as shown in  FIG.  11   . 
     The objective of the present invention is also to provide a crystal form of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. 
     In a further preferred embodiment of the present invention, crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is provided. 
     The X-ray powder diffraction pattern of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine has a diffraction peak at 2θ of 20.0±0.2°; or has a diffraction peak at 19.4±0.2°; or has a diffraction peak at 17.1±0.2°; or has a diffraction peak at 22.6±0.2°; or has a diffraction peak at 10.7±0.2°; or has a diffraction peak at 25.6±0.2°; or has a diffraction peak at 12.7±0.2°; or has a diffraction peak at 24.5±0.2°; or has a diffraction peak at 19.1±0.2°; or has a diffraction peak at 18.4±0.2°; preferably comprises any 2 to 5, or 3 to 5, or 3 to 6, or 3 to 8, or 5 to 8, or 6 to 8 of the above diffraction peaks; and more preferably comprises any 6, 7, or 8 of the above diffraction peaks. 
     The X-ray powder diffraction pattern of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine at least comprises one or more diffraction peaks at 2θ of 20.0±0.2°, 19.4±0.2° and 17.1±0.2°, preferably comprises 2 of the above diffraction peaks, and more preferably comprises 3 of the above diffraction peaks; optionally, can further comprises at least one diffraction peak at 2θ of 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2, and preferably comprises 2, 3, 4, or 5 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form A has diffraction peaks at 2θ of the following positions: 
     20.0±0.2° and 19.4±0.2°;
 
or, 19.4±0.2° and 17.1±0.2°;
 
or, 20.0±0.2° and 17.1±0.2°;
 
or, 20.0±0.2° and 22.6±0.2°;
 
or, 22.6±0.2° and 10.7±0.2°;
 
or, 20.0±0.2° and 10.7±0.2°;
 
or, 10.7±0.2° and 25.6±0.2°;
 
or, 10.7±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2° and 17.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2° and 22.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2° and 25.6±0.2°;
 
or, 19.4±0.2°, 17.1±0.2° and 25.6±0.2°;
 
or, 19.4±0.2°, 17.1±0.2° and 22.6±0.2°;
 
or, 19.4±0.2°, 17.1±0.2° and 10.7±0.2°;
 
or, 17.1±0.2°, 22.6±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 10.7±0.2° and 22.6±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 10.7±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 10.7±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 10.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 25.6±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 25.6±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and
 
24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 25.6±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 17.1±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 10.7±0.2°.
 
     The X-ray powder diffraction pattern of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine optionally also comprises one or more diffraction peaks at 2θ of 19.1±0.2°, 18.4±0.2°, 6.2±0.2°, 31.5±0.2°, 29.2±0.2°, 20.6±0.2° and 21.4±0.2°; preferably at least comprises any 2 to 3, or 4 to 5, or 6 to 7 of the above diffraction peaks; and further preferably comprises any 2, 3, 4, 5, 6, or 7 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form A has diffraction peaks at 2θ of the following positions: 
     20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 24.5±0.2°, 18.4±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 12.7±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 24.5±0.2°, 18.4±0.2° and
 
19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 18.4±0.2° and
 
19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 18.4±0.2° and
 
19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°,
 
19.1±0.2° and 18.4±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and
 
19.1±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 29.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 19.1±0.2° and 18.4±0.2°.
 
     The X-ray powder diffraction pattern of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine comprises one or more diffraction peaks at 2θ of 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2, 19.1±0.2°, 18.4±0.2°, 6.2±0.2°, 31.5±0.2°, 29.2±0.2°, 20.6±0.2° and 21.4±0.2°; and preferably comprises any 4, 5, 6, 8, or 10 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form A has diffraction peaks at 2θ of the following positions: 
     20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 22.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2° and 31.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 10.7±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 25.6±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 19.1±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 18.4±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 6.2±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2° and 31.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 25.6±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 12.7±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 24.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 19.1±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 18.4±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 6.2±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 24.5±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and 19.1±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2° and 18.4±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 18.4±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 18.4±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 6.2±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 18.4±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 19.1±0.2°, 18.4±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 19.1±0.2°, 18.4±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 19.1±0.2°, 6.2±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 19.1±0.2°, 18.4±0.2°, 6.2±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 6.2±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2°, 6.2±0.2° and 31.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2° and 6.2±0.2°;
 
or, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2°, 6.2±0.2° and 31.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2°, 6.2±0.2° and 31.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2°, 6.2±0.2° and 31.5±0.2°.
 
     The X-ray powder diffraction pattern of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine comprises one or more diffraction peaks at 2θ of 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2, 19.1±0.2°, 18.4±0.2°, 6.2±0.2°, 31.5±0.2°, 29.2±0.2°, 20.6±0.2°, 21.4±0.2°, 21.1±0.2°, 15.8±0.2°, 27.4±0.2°, 33.1±0.2° and 14.5±0.2°; preferably at least comprises any 2 to 3, or 4 to 5, or 7 to 8, or 10 to 12, or 15 to 18 of the above diffraction peaks; and further preferably comprises any 2, 3, 4, 5, 6, 8, 10, 12, 16, 18 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form A has diffraction peaks at 2θ of the following positions: 
     20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 22.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 10.7±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 25.6±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 25.6±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 10.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 25.6±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 10.7±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 12.7±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 21.1±0.2°, 15.8±0.2° and 24.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 24.5±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 24.5±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 19.1±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 19.1±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 21.1±0.2°, 15.8±0.2° and 19.1±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 25.6±0.2°, 24.5±0.2°, 19.1±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 21.1±0.2°, 15.8±0.2° and 18.4±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 18.4±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 18.4±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 6.2±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 24.5±0.2°, 18.4±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 19.1±0.2°, 18.4±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 19.1±0.2°, 18.4±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 12.7±0.2°, 19.1±0.2°, 6.2±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 25.6±0.2°, 19.1±0.2°, 18.4±0.2°, 6.2±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2°, 21.1±0.2°, 15.8±0.2° and 6.2±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 12.7±0.2°, 24.5±0.2°, 19.1±0.2°, 6.2±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°;
 
or, 20.0±0.2°, 19.4±0.2°, 17.1±0.2°, 22.6±0.2°, 10.7±0.2°, 24.5±0.2°, 19.1±0.2°, 18.4±0.2°, 6.2±0.2°, 21.1±0.2°, 15.8±0.2° and 31.5±0.2°.
 
     Most preferably, using Cu—Kα radiation, the characteristic X-ray diffraction peaks represented by 2θ angle and interplanar spacing d value are shown in Table 6. 
     
       
         
           
               
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                   
                 XRPD diffraction data of crystal form A 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Peak  
                 Proportion  
               
               
                 No. 
                 2θ (±0.2°) 
                 d value 
                 height 
                 (I %) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 1 
                 6.205 
                 14.2327 
                 335 
                 15.2 
               
               
                 2 
                 10.747 
                 8.2255 
                 727 
                 33 
               
               
                 3 
                 12.715 
                 6.9565 
                 630 
                 28.6 
               
               
                 4 
                 14.545 
                 6.0847 
                 184 
                 8.4 
               
               
                 5 
                 15.761 
                 5.6181 
                 218 
                 9.9 
               
               
                 6 
                 17.116 
                 5.1763 
                 1073 
                 48.7 
               
               
                 7 
                 18.374 
                 4.8247 
                 365 
                 16.6 
               
               
                 8 
                 19.061 
                 4.6521 
                 424 
                 19.3 
               
               
                 9 
                 19.428 
                 4.5651 
                 1271 
                 57.7 
               
               
                 10 
                 19.976 
                 4.4411 
                 2202 
                 100 
               
               
                 11 
                 20.606 
                 4.3067 
                 278 
                 12.6 
               
               
                 12 
                 21.111 
                 4.2048 
                 229 
                 10.4 
               
               
                 13 
                 21.44 
                 4.141 
                 264 
                 12 
               
               
                 14 
                 22.551 
                 3.9396 
                 838 
                 38.1 
               
               
                 15 
                 24.532 
                 3.6257 
                 470 
                 21.3 
               
               
                 16 
                 25.612 
                 3.4751 
                 717 
                 32.6 
               
               
                 17 
                 27.379 
                 3.2548 
                 196 
                 8.9 
               
               
                 18 
                 29.22 
                 3.0537 
                 318 
                 14.4 
               
               
                 19 
                 31.514 
                 2.8365 
                 333 
                 15.1 
               
               
                 20 
                 33.054 
                 2.7078 
                 191 
                 8.7 
               
               
                   
               
            
           
         
       
     
     The crystal form of the present invention is crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, the X-ray powder diffraction pattern thereof is substantially as shown in  FIG.  12   ; the DSC spectrum thereof is as shown in  FIG.  13   ; and the TGA spectrum thereof is as shown in  FIG.  14   . 
     In a preferred embodiment of the present invention, crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a solvent-containing crystal form or a solvent-free crystal form, wherein the solvent is one or more selected from the group consisting of water, methanol, acetone, ethyl acetate, acetonitrile, ethanol, 8800 acetone, tetrahydrofuran, 2-methyl-tetrahydrofuran, dichloromethane, 1,4-dioxane, benzene, toluene, isopropanol, n-butanol, isobutanol, N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidone, dimethyl sulfoxide, n-propanol, tert-butanol, 2-butanone, 3-pentanone, n-heptane, heptane, ethyl formate, isopropyl acetate, cyclohexane, methyl tert-butyl ether and isopropyl ether. 
     In a preferred embodiment of the present invention, the number of solvent molecule contained in crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is 0.2 to 3, preferably 0.2, 0.5, 1, 1.5, 2, 2.5 or 3, and more preferably 0.5, 1, 2 or 3. 
     In a preferred embodiment of the present invention, crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a solvent-free crystal form, and preferably an anhydrous crystal form. 
     In a preferred embodiment of the present invention, crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a hydrate crystal form, and the number of water molecule is 0.2 to 3, preferably 0.2, 0.5, 1, 1.5, 2, 2.5 or 3, and more preferably 0.5, 1, 2 or 3. 
     In a further preferred embodiment of the present invention, crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is provided. 
     The X-ray powder diffraction pattern of crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine has a diffraction peak at 2θ of 20.2±0.2°; or has a diffraction peak at 22.2±0.2°; or has a diffraction peak at 12.2±0.2°; or has a diffraction peak at 25.1±0.2°; or has a diffraction peak at 21.5±0.2°; or has a diffraction peak at 18.5±0.2°; or has a diffraction peak at 17.8±0.2°; or has a diffraction peak at 20.0±0.2°; or has a diffraction peak at 28.6±0.2°; or has a diffraction peak at 5.1±0.2°; preferably comprises any 2 to 5, or 3 to 5, or 3 to 6, or 3 to 8, or 5 to 8, or 6 to 8 of the above diffraction peaks; and more preferably comprises any 6, 7, or 8 of the above diffraction peaks; 
     the X-ray powder diffraction pattern of crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine at least comprises one or more diffraction peaks at 2θ of 20.2±0.2°, 22.2±0.2° and 12.2±0.2°, preferably comprises 2 of the above diffraction peaks, and more preferably comprises 3 of the above diffraction peaks; optionally, can further comprises at least one diffraction peak at 2θ of 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, and preferably comprises 2, 3, 4, or 5 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form B has diffraction peaks at 2θ of the following positions: 
     20.2±0.2° and 22.2±0.2°;
 
or, 20.2±0.2° and 12.2±0.2°;
 
or, 22.2±0.2° and 12.2±0.2°;
 
or, 22.2±0.2° and 25.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2° and 25.1±0.2°;
 
or, 22.2±0.2°, 25.1±0.2° and 21.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2° and 21.5±0.2°;
 
or, 20.2±0.2°, 12.2±0.2° and 25.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2° and 25.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 25.1±0.2° and 21.5±0.2°;
 
or, 20.2±0.2°, 12.2±0.2°, 25.1±0.2° and 21.5±0.2°;
 
or, 22.2±0.2°, 25.1±0.2°, 21.5±0.2° and 18.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2° and 21.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 25.1±0.2°, 21.5±0.2° and 18.5±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 18.5±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 18.5±0.2° and 17.8±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 18.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2° and 17.8±0.2°;
 
or, 20.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2° and 17.8±0.2°;
 
or, 12.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 18.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 17.8±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2° and 20.0±0.2°;
 
or, 20.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2° and 20.0±0.2°;
 
or, 12.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 17.8±0.2°.
 
     The X-ray powder diffraction pattern of crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine optionally also comprises one or more diffraction peaks at 2θ of 28.6±0.2°, 5.1±0.2°, 14.3±0.2°, 24.5±0.2°, 13.2±0.2°, 12.4±0.2° and 29.2±0.2°; preferably at least comprises any 2 to 3, or 4 to 5, or 6 to 7 of the above diffraction peaks; and further preferably comprises any 2, 3, 4, 5, 6, or 7 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form B has diffraction peaks at 2θ of the following positions: 
     20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2° and 5.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 28.6±0.2°, 5.1±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 28.6±0.2°, 5.1±0.2°, 14.3±0.2° and 24.5±0.2°;
 
20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 28.6±0.2°, 5.1±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 28.6±0.2°, 5.1±0.2°, 14.3±0.2° and 13.2±0.2°;
 
20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 28.6±0.2°, 5.1±0.2° and 13.2±0.2°;
 
or, 20.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 28.6±0.2°, 5.1±0.2°, 14.3±0.2° and 12.4±0.2°.
 
     The X-ray powder diffraction pattern of crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine comprises one or more diffraction peaks at 2θ of 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2°, 5.1±0.2°, 14.3±0.2°, 24.5±0.2°, 13.2±0.2°, 12.4±0.2° and 29.2±0.2°; and preferably comprises any 4, 5, 6, 8, or 10 of the above diffraction peaks;
         for example, the X-ray powder diffraction pattern of crystal form B has diffraction peaks at 2θ of the following positions:
 
20.2±0.2°, 22.2±0.2°, 12.2±0.2° and 25.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2° and 21.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2° and 18.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2° and 17.8±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2° and 20.0±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2° and 22.2±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2° and 21.5±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 21.5±0.2° and 18.5±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 18.5±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 18.5±0.2° and 28.6±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 28.6±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 21.5±0.2°, 18.5±0.2° and 28.6±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 5.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 21.5±0.2°, 18.5±0.2° and 5.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 21.5±0.2°, 18.5±0.2° and 14.3±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2° and 28.6±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 28.6±0.2° and 14.3±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2° and 14.3±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 5.1±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 5.1±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2° and 13.2±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 5.1±0.2° and 13.2±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2 and 28.6±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 28.6±0.2° and 14.3±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2° and 14.3±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 5.1±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 5.1±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2° and 13.2±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 5.1±0.2° and 13.2±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2°, 5.1±0.2° and 13.2±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2°, 5.1±0.2° and 14.3±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2°, 5.1±0.2°, 14.3±0.2° and 13.2±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2°, 5.1±0.2°, 14.3±0.2° and 24.5±0.2°.
       

     The X-ray powder diffraction pattern of crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine comprises one or more diffraction peaks at 2θ of 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2, 28.6±0.2°, 5.1±0.2°, 14.3±0.2°, 24.5±0.2°, 13.2±0.2°, 12.4±0.2°, 29.2±0.2°, 10.8±0.2°, 9.9±0.2°, 16.9±0.2°, 30.5±0.2° and 15.5±0.2°; preferably at least comprises any 2 to 3, or 4 to 5, or 7 to 8, or 10 to 12, or 15 to 18 of the above diffraction peaks; and further preferably comprises any 2, 3, 4, 5, 6, 8, 10, 12, 16, 18 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form B has diffraction peaks at 2θ of the following positions: 
     20.2±0.2°, 22.2±0.2°, 12.2±0.2° and 25.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 10.8±0.2°, 9.9±0.2° and 21.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 10.8±0.2°, 9.9±0.2° and 18.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 10.8±0.2°, 9.9±0.2° and 17.8±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 10.8±0.2°, 9.9±0.2° and 20.0±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 10.8±0.2°, 9.9±0.2°, 10.8±0.2° and 9.9±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 10.8±0.2°, 10.8±0.2°, 9.9±0.2° and 22.2±0.2°;
 
or, 12.2±0.2°, 10.8±0.2°, 9.9±0.2°, 10.8±0.2°, 9.9±0.2° and 22.2±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 10.8±0.2°, 9.9±0.2° and 28.6±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 10.8±0.2°, 9.9±0.2°, 10.8±0.2°, 9.9±0.2° and
 
22.2±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 10.8±0.2°, 9.9±0.2° and 28.6±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 21.5±0.2°, 18.5±0.2°, 10.8±0.2°, 9.9±0.2° and 28.6±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 10.8±0.2°, 9.9±0.2° and 5.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 21.5±0.2°, 18.5±0.2°, 10.8±0.2°, 9.9±0.2° and 5.1±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 10.8±0.2°, 9.9±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 21.5±0.2°, 18.5±0.2°, 10.8±0.2°, 9.9±0.2° and 14.3±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2°, 10.8±0.2° and 9.9±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2°, 9.9±0.2° and 14.3±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2°, 10.8±0.2°, 9.9±0.2° and 14.3±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2°, 10.8±0.2°, 9.9±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2°, 10.8±0.2°, 9.9±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 5.1±0.2°, 10.8±0.2°, 9.9±0.2° and 14.3±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2°, 10.8±0.2°, 9.9±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 5.1±0.2°, 10.8±0.2°, 9.9±0.2° and 24.5±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 28.6±0.2°, 10.8±0.2°, 9.9±0.2° and 13.2±0.2°;
 
or, 20.2±0.2°, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 5.1±0.2°, 10.8±0.2°, 9.9±0.2° and 13.2±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 28.6±0.2°, 10.8±0.2°, 9.9±0.2° and 13.2±0.2°;
 
or, 22.2±0.2°, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2°, 10.8±0.2°, 9.9±0.2° and 13.2±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2°, 28.6±0.2°, 10.8±0.2°, 9.9±0.2° and 13.2±0.2°;
 
or, 12.2±0.2°, 25.1±0.2°, 21.5±0.2°, 18.5±0.2°, 17.8±0.2°, 20.0±0.2°, 28.6±0.2°, 5.1±0.2°, 9.9±0.2° and 13.2±0.2°.
 
     Most preferably, using Cu—Kα radiation, the characteristic X-ray diffraction peaks represented by 2θ angle and interplanar spacing d value are shown in Table 7. 
     
       
         
           
               
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                   
                 XRPD diffraction data of crystal form B 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Peak  
                 Proportion  
               
               
                 No. 
                 2θ (±0.2°) 
                 d value 
                 height 
                 (I %) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 1 
                 5.095 
                 17.3287 
                 325 
                 22 
               
               
                 2 
                 9.939 
                 8.892 
                 189 
                 12.8 
               
               
                 3 
                 10.831 
                 8.162 
                 191 
                 12.9 
               
               
                 4 
                 12.188 
                 7.256 
                 815 
                 55.2 
               
               
                 5 
                 12.414 
                 7.1243 
                 231 
                 15.7 
               
               
                 6 
                 13.162 
                 6.7211 
                 265 
                 18 
               
               
                 7 
                 14.316 
                 6.1817 
                 306 
                 20.7 
               
               
                 8 
                 15.493 
                 5.7145 
                 173 
                 11.7 
               
               
                 9 
                 16.854 
                 5.2563 
                 179 
                 12.1 
               
               
                 10 
                 17.788 
                 4.9822 
                 371 
                 25.1 
               
               
                 11 
                 18.452 
                 4.8045 
                 440 
                 29.8 
               
               
                 12 
                 19.976 
                 4.4412 
                 359 
                 24.3 
               
               
                 13 
                 20.238 
                 4.3842 
                 1476 
                 100 
               
               
                 14 
                 21.455 
                 4.1382 
                 502 
                 34 
               
               
                 15 
                 22.185 
                 4.0037 
                 1166 
                 79 
               
               
                 16 
                 24.496 
                 3.631 
                 295 
                 20 
               
               
                 17 
                 25.081 
                 3.5476 
                 634 
                 43 
               
               
                 18 
                 28.551 
                 3.1238 
                 327 
                 22.2 
               
               
                 19 
                 29.239 
                 3.0518 
                 216 
                 14.6 
               
               
                 20 
                 30.478 
                 2.9305 
                 177 
                 12 
               
               
                   
               
            
           
         
       
     
     The crystal form of the present invention is crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, the X-ray powder diffraction pattern thereof is substantially as shown in  FIG.  15   ; and the DSC spectrum thereof is as shown in  FIG.  16   . 
     In a preferred embodiment of the present invention, crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a solvent-containing crystal form or a solvent-free crystal form, wherein the solvent is one or more selected from the group consisting of water, methanol, acetone, ethyl acetate, acetonitrile, ethanol, 88% acetone, tetrahydrofuran, 2-methyl-tetrahydrofuran, dichloromethane, 1,4-dioxane, benzene, toluene, isopropanol, n-butanol, isobutanol, N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidone, dimethyl sulfoxide, n-propanol, tert-butanol, 2-butanone, 3-pentanone, n-heptane, heptane, ethyl formate, isopropyl acetate, cyclohexane, methyl tert-butyl ether and isopropyl ether. 
     In a preferred embodiment of the present invention, the number of solvent molecule contained in crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is 0.2 to 3, preferably 0.2, 0.5, 1, 1.5, 2, 2.5 or 3, and more preferably 0.5, 1, 2 or 3. 
     In a preferred embodiment of the present invention, crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a solvent-free crystal form, and preferably an anhydrous crystal form. 
     In a preferred embodiment of the present invention, crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a hydrate crystal form, and the number of water molecule is 0.2 to 3, preferably 0.2, 0.5, 1, 1.5, 2, 2.5 or 3, and more preferably 0.5, 1, 2 or 3. 
     In a further preferred embodiment of the present invention, crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is provided. 
     The X-ray powder diffraction pattern of crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine has a diffraction peak at 2θ of 17.8±0.2°; or has a diffraction peak at 19.8±0.2°; or has a diffraction peak at 26.1±0.2°; or has a diffraction peak at 18.2±0.2°; or has a diffraction peak at 24.0±0.2°; or has a diffraction peak at 22.8±0.2°; or has a diffraction peak at 10.5±0.2°; or has a diffraction peak at 21.5±0.2°; or has a diffraction peak at 17.5±0.2°; or has a diffraction peak at 21.8±0.2°; preferably comprises any 2 to 5, or 3 to 5, or 3 to 6, or 3 to 8, or 5 to 8, or 6 to 8 of the above diffraction peaks; and more preferably comprises any 6, 7, or 8 of the above diffraction peaks; 
     the X-ray powder diffraction pattern of crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine at least comprises one or more diffraction peaks at 2θ of 17.8±0.2°, 19.8±0.2° and 26.1±0.2°, preferably comprises 2 of the above diffraction peaks, and more preferably comprises 3 of the above diffraction peaks; optionally, can further comprises at least one diffraction peak at 2θ of 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, and preferably comprises 2, 3, 4, or 5 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form C has diffraction peaks at 2θ of the following positions: 
     17.8±0.2° and 19.8±0.2°;
 
or, 17.8±0.2° and 26.1±0.2°;
 
or, 19.8±0.2° and 26.1±0.2°;
 
or, 26.1±0.2° and 18.2±0.2°;
 
or, 17.8±0.2°, 19.8±0.2° and 26.1±0.2°;
 
or, 17.8±0.2°, 26.1±0.2° and 18.2±0.2°;
 
or, 19.8±0.2°, 26.1±0.2° and 18.2±0.2°;
 
or, 26.1±0.2°, 18.2±0.2° and 24.0±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2° and 18.2±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 18.2±0.2° and 24.0±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2° and 24.0±0.2°;
 
or, 26.1±0.2°, 18.2±0.2°, 24.0±0.2° and 22.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2° and 24.0±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2° and 22.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 18.2±0.2°, 24.0±0.2° and 10.5±0.2°;
 
or, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2° and 10.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2° and 22.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2° and 10.5±0.2°;
 
or, 17.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2° and 10.5±0.2°;
 
or, 26.1±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2° and 22.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2° and 10.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2° and 21.5±0.2°;
 
or, 17.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2° and 21.5±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2° and 10.5±0.2°.
 
     The X-ray powder diffraction pattern of crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine optionally also comprises one or more diffraction peaks at 2θ of 17.5±0.2°, 21.8±0.2°, 19.5±0.2°, 22.3±0.2°, 24.3±0.2°, 14.5±0.2° and 30.1±0.2°; preferably at least comprises any 2 to 3, or 4 to 5, or 6 to 7 of the above diffraction peaks; and further preferably comprises any 2, 3, 4, 5, 6, or 7 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form C has diffraction peaks at 2θ of the following positions: 
     17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2° and 21.8±0.2°;
 
or, 19.8±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2° and 19.5±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2° and 22.3±0.2°;
 
or, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2°, 19.5±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2°, 19.5±0.2° and 22.3±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2°, 19.5±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2° and 19.5±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2° and 22.3±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2°, 19.5±0.2° and 24.3±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2°, 19.5±0.2° and 24.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2° and 22.3±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2°, 21.8±0.2°, and 22.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2° and 24.3±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2° and 24.3±0.2°;
 
or, 17.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2°, 19.5±0.2° and 14.5±0.2°;
 
or, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 17.5±0.2°, 21.8±0.2°, 19.5±0.2° and 14.5±0.2°.
 
     The X-ray powder diffraction pattern of crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine comprises one or more diffraction peaks at 2θ of 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, 17.5±0.2°, 21.8±0.2°, 19.5±0.2°, 22.3±0.2°, 24.3±0.2°, 14.5±0.2° and 30.1±0.2°; and preferably comprises any 4, 5, 6, 8, or 10 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form C has diffraction peaks at 2θ of the following positions: 
     17.8±0.2°, 19.8±0.2°, 26.1±0.2° and 18.2±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2° and 24.0±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2° and 22.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2° and 10.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2° and 21.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2° and 17.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2° and 17.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 24.0±0.2°, 22.8±0.2° and 17.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2° and 21.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 24.0±0.2°, 22.8±0.2° and 21.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 24.0±0.2°, 22.8±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 21.8±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 21.8±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2° and 24.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 21.8±0.2° and 24.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, 17.5±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, 21.8±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, 17.5±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, 21.8±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, 17.5±0.2° and 24.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, 21.8±0.2° and 24.3±0.2°.
 
     The X-ray powder diffraction pattern of crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine comprises one or more diffraction peaks at 2θ of 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 10.5±0.2°, 21.5±0.2, 17.5±0.2°, 21.8±0.2°, 19.5±0.2°, 22.3±0.2°, 24.3±0.2°, 14.5±0.2°, 30.1±0.2°, 23.4±0.2°, 12.0±0.2°, 24.8±0.2°, 13.2±0.2° and 12.5±0.2°; preferably at least comprises any 2 to 3, or 4 to 5, or 7 to 8, or 10 to 12, or 15 to 18 of the above diffraction peaks; and further preferably comprises any 2, 3, 4, 5, 6, 8, 10, 12, 16, 18 of the above diffraction peaks; 
     for example, the X-ray powder diffraction pattern of crystal form C has diffraction peaks at 2θ of the following positions: 
     17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 23.4±0.2°, 12.0±0.2° and 18.2±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 23.4±0.2°, 12.0±0.2° and 24.0±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 23.4±0.2°, 12.0±0.2° and 22.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 23.4±0.2°, 12.0±0.2° and 10.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 23.4±0.2°, 12.0±0.2° and 21.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 23.4±0.2°, 12.0±0.2° and 17.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 23.4±0.2°, 12.0±0.2° and
 
17.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 24.0±0.2°, 22.8±0.2°, 23.4±0.2°, 12.0±0.2° and 17.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 23.4±0.2°, 12.0±0.2° and 21.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 24.0±0.2°, 22.8±0.2°, 23.4±0.2°, 12.0±0.2° and 21.8±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 23.4±0.2°, 12.0±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 24.0±0.2°, 22.8±0.2°, 23.4±0.2°, 12.0±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2°, 23.4±0.2°, 12.0±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 21.8±0.2°, 23.4±0.2°, 12.0±0.2° and 19.5±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2°, 23.4±0.2°, 12.0±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 21.8±0.2°, 23.4±0.2°, 12.0±0.2° and 22.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 17.5±0.2°, 23.4±0.2°, 12.0±0.2° and 24.3±0.2°;
 
or, 17.8±0.2°, 19.8±0.2°, 26.1±0.2°, 18.2±0.2°, 24.0±0.2°, 22.8±0.2°, 21.8±0.2°, 23.4±0.2°, 12.0±0.2° and 24.3±0.2°.
 
     Most preferably, using Cu—Kα radiation, the characteristic X-ray diffraction peaks represented by 2θ angle and interplanar spacing d value are shown in Table 8. 
     
       
         
           
               
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                   
                 XRPD diffraction data of crystal form C 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 Peak  
                 Proportion  
               
               
                 No. 
                 2θ (±0.2°) 
                 d value 
                 height 
                 (I %) 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 1 
                 10.544 
                 8.3832 
                 296 
                 36.4 
               
               
                 2 
                 12.046 
                 7.3412 
                 138 
                 17 
               
               
                 3 
                 12.476 
                 7.0891 
                 120 
                 14.7 
               
               
                 4 
                 13.182 
                 6.7106 
                 129 
                 15.8 
               
               
                 5 
                 14.461 
                 6.1201 
                 173 
                 21.3 
               
               
                 6 
                 17.522 
                 5.0572 
                 242 
                 29.7 
               
               
                 7 
                 17.807 
                 4.9769 
                 814 
                 100 
               
               
                 8 
                 18.249 
                 4.8573 
                 541 
                 66.5 
               
               
                 9 
                 19.451 
                 4.5598 
                 210 
                 25.8 
               
               
                 10 
                 19.835 
                 4.4725 
                 607 
                 74.6 
               
               
                 11 
                 21.473 
                 4.1349 
                 287 
                 35.3 
               
               
                 12 
                 21.762 
                 4.0806 
                 233 
                 28.6 
               
               
                 13 
                 22.306 
                 3.9822 
                 201 
                 24.7 
               
               
                 14 
                 22.813 
                 3.8949 
                 341 
                 41.9 
               
               
                 15 
                 23.4 
                 3.7984 
                 156 
                 19.2 
               
               
                 16 
                 24.049 
                 3.6974 
                 371 
                 45.6 
               
               
                 17 
                 24.332 
                 3.655 
                 198 
                 24.3 
               
               
                 18 
                 24.798 
                 3.5873 
                 132 
                 16.2 
               
               
                 19 
                 26.117 
                 3.4091 
                 567 
                 69.7 
               
               
                 20 
                 30.11 
                 2.9655 
                 160 
                 19.7 
               
               
                   
               
            
           
         
       
     
     The crystal form of the present invention is crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, the X-ray powder diffraction pattern thereof is substantially as shown in  FIG.  17   ; and the DSC spectrum thereof is as shown in  FIG.  18   . 
     In a preferred embodiment of the present invention, crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a solvent-containing crystal form or a solvent-free crystal form, wherein the solvent is one or more selected from the group consisting of water, methanol, acetone, ethyl acetate, acetonitrile, ethanol, 88% acetone, tetrahydrofuran, 2-methyl-tetrahydrofuran, dichloromethane, 1,4-dioxane, benzene, toluene, isopropanol, n-butanol, isobutanol, N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidone, dimethyl sulfoxide, n-propanol, tert-butanol, 2-butanone, 3-pentanone, n-heptane, heptane, ethyl formate, isopropyl acetate, cyclohexane, methyl tert-butyl ether and isopropyl ether. 
     In a preferred embodiment of the present invention, the number of solvent molecule contained in crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is 0.2 to 3, preferably 0.2, 0.5, 1, 1.5, 2, 2.5 or 3, and more preferably 0.5, 1, 2 or 3. 
     In a preferred embodiment of the present invention, crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a solvent-free crystal form, and preferably an anhydrous crystal form. 
     In a preferred embodiment of the present invention, crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is a hydrate crystal form, and the number of water molecule is 0.2 to 3, preferably 0.2, 0.5, 1, 1.5, 2, 2.5 or 3, and more preferably 0.5, 1, 2 or 3. 
     In a further preferred embodiment of the present invention, in the X-ray powder diffraction patterns of the crystal forms, the 20 error between the diffraction peak position having top-ten relative peak intensity and the reference diffraction peak position is ±0.2° to ±0.5°, preferably ±0.2° to ±0.3°, and most preferably ±0.2°. In a further preferred embodiment of the present invention, in the X-ray powder diffraction patterns of crystal form A, crystal form B and crystal form C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, the 20 error between the diffraction peak having top-ten relative peak intensity and the reference diffraction peak position in  FIG.  1   ,  FIG.  4    and  FIG.  6    respectively is ±0.2° to ±0.5°, preferably ±0.2° to ±0.3°, and most preferably ±0.2°. 
     The present invention also provides a method for preparing the crystal form of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, specifically comprising the following steps of: 
     1) weighing an appropriate amount of free base of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, and suspending it in a poor solvent, the suspension density is preferably 50 to 200 mg/mL; 
     2) shaking the suspension obtained in step 1) at a certain temperature for a certain period of time, the temperature is preferably 0 to 60° C., and the time is preferably 1 to 10 days; 
     3) rapidly centrifuging the suspension obtained in step 2), removing the supernatant, and drying the remaining solid in a vacuum drying oven at 40° C. to constant weight to obtain the target product; 
     wherein: 
     the poor solvent is one or more selected from the group consisting of acetone, ethyl acetate, isopropyl acetate, acetonitrile, ethanol, 88% acetone, tetrahydrofuran, 2-methyltetrahydrofuran, dichloromethane, 1,4-dioxane, benzene, toluene, isopropanol, n-butanol, isobutanol, N,N-dimethylformamide, N,N-dimethylacetamide, n-propanol, tert-butanol, 2-butanone, 3-pentanone, methyl tert-butyl ether and water, and preferably one or more selected from the group consisting of acetone, ethyl acetate, isopropyl acetate, acetonitrile, ethanol, 88% acetone, tetrahydrofuran, 2-methyltetrahydrofuran, dichloromethane, 1,4-dioxane, toluene, isopropanol, 2-butanone, 3-pentanone, methyl tert-butyl ether and water; 
     or, specifically comprising the following steps of: 
     1) weighing an appropriate amount of free base of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, and dissolving it in a good solvent; 
     2) adding an anti-solvent to the solution obtained in step 1) at a certain temperature, and stirring the solution until a solid is precipitated, the temperature is preferably 0 to 25° C.; 
     3) rapidly centrifuging the suspension obtained in step 2), removing the supernatant, and drying the remaining solid in a vacuum drying oven at 40° C. to constant weight to obtain the target product; 
     wherein: 
     the good solvent is one or more selected from the group consisting of methanol, acetone, ethyl acetate, acetonitrile, ethanol, 88% acetone, tetrahydrofuran, 2-methyltetrahydrofuran, dichloromethane, 1,4-dioxane, benzene, toluene, isopropanol, n-butanol, isobutanol, N,N-dimethylformamide, N,N-dimethylacetamide, n-propanol, tert-butanol, 2-butanone, 3-pentanone and N-methylpyrrolidone, and preferably N-methylpyrrolidone; 
     the anti-solvent is selected from the group consisting of heptane, water, methyl tert-butyl ether, toluene and isopropyl ether; 
     or, specifically comprising the following steps of: 
     1) weighing an appropriate amount of free base of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, and dissolving it in a good solvent under heating; 
     2) rapidly placing the solution obtained in step 1) at a low temperature, and stirring it until a solid is precipitated, the temperature is preferably −10 to 5° C.; 
     3) rapidly centrifuging the suspension obtained in step 2), removing the supernatant, and drying the remaining solid in a vacuum drying oven at 40° C. to constant weight to obtain the target product; 
     wherein: 
     the good solvent is one or more selected from the group consisting of methanol, acetone, ethyl acetate, acetonitrile, ethanol, 88% acetone, tetrahydrofuran, 2-methyltetrahydrofuran, dichloromethane, 1,4-dioxane, benzene, toluene, isopropanol, n-butanol, isobutanol, N,N-dimethylformamide, N,N-dimethylacetamide, n-propanol, tert-butanol, 2-butanone, 3-pentanone and N-methylpyrrolidone, and preferably 2-methyltetrahydrofuran. 
     The objective of the present invention is also to provide a pharmaceutical composition comprising a therapeutically effective amount of the crystal form of the compound of formula (I) or the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, and one or more pharmaceutically acceptable carrier(s), diluent(s) or excipient(s). 
     The objective of the present invention is also to provide a pharmaceutical composition comprising a therapeutically effective amount of the crystal form of the compound of formula (I) or the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, and one or more pharmaceutically acceptable carrier(s), diluent(s) or excipient(s). 
     The objective of the present invention is also to provide a use of the crystal form of the compound of formula (I) or the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine or the pharmaceutical composition comprising the same in the preparation of a SHP-2 inhibitor medicament. 
     The objective of the present invention is also to provide a use of the crystal form of the compound of formula (I) or the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine or the pharmaceutical composition comprising the same in the preparation of a SHP-2 inhibitor medicament. 
     In the above use, the use is a use in the preparation of a medicament for treating a disease or condition such as Noonan syndrome, leopard skin syndrome, leukemia, neuroblastoma, melanoma, esophageal cancer, head and neck tumor, breast cancer, lung cancer and colon cancer; and preferably non-small cell lung cancer, esophageal cancer or head and neck tumor. 
    
    
     
       DESCRIPTION OF THE DRAWINGS 
         FIG.  1    is the DSC spectrum of crystal form A of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  2    is the TGA spectrum of crystal form A of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  3    is the XRPD pattern of crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  4    is the DSC spectrum of crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  5    is the TGA spectrum of crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  6    is the DSC spectrum of crystal form C of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  7    is the TGA spectrum of crystal form C of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  8    is the DSC spectrum of crystal form D of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  9    is the TGA spectrum of crystal form D of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  10    is the DSC spectrum of crystal form E of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  11    is the TGA spectrum of crystal form E of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. 
         FIG.  12    is the XRPD pattern of crystal form A of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. 
         FIG.  13    is the DSC spectrum of crystal form A of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. 
         FIG.  14    is the TGA spectrum of crystal form A of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. 
         FIG.  15    is the XRPD pattern of crystal form B of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. 
         FIG.  16    is the DSC spectrum of crystal form B of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. 
         FIG.  17    is the XRPD pattern of crystal form C of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. 
         FIG.  18    is the DSC spectrum of crystal form C of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Unless otherwise stated, the terms used in the specification and claims have the meanings described below. 
     The term “alkyl” refers to a saturated aliphatic hydrocarbon group, which is a straight or branched chain group comprising 1 to 20 carbon atoms, preferably an alkyl having 1 to 8 carbon atoms, more preferably an alkyl having 1 to 6 carbon atoms, and most preferably an alkyl having 1 to 3 carbon atoms. Non-limiting examples include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl, n-pentyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, 2-methylbutyl, 3-methylbutyl, n-hexyl, 1-ethyl-2-methylpropyl, 1,1,2-trimethylpropyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1,3-dimethylbutyl, 2-ethylbutyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 2,3-dimethylbutyl, n-heptyl, 2-methylhexyl, 3-methylhexyl, 4-methylhexyl, 5-methylhexyl, 2,3-dimethylpentyl, 2,4-dimethylpentyl, 2,2-dimethylpentyl, 3,3-dimethylpentyl, 2-ethylpentyl, 3-ethylpentyl, n-octyl, 2,3-dimethylhexyl, 2,4-dimethylhexyl, 2,5-dimethylhexyl, 2,2-dimethylhexyl, 3,3-dimethylhexyl, 4,4-dimethylhexyl, 2-ethylhexyl, 3-ethylhexyl, 4-ethylhexyl, 2-methyl-2-ethylpentyl, 2-methyl-3-ethylpentyl, n-nonyl, 2-methyl-2-ethylhexyl, 2-methyl-3-ethylhexyl, 2,2-diethylpentyl, n-decyl, 3,3-diethylhexyl, 2,2-diethylhexyl, and various branched isomers thereof. The alkyl group can be substituted or unsubstituted. 
     When substituted, the substituent group(s) can be substituted at any available connection point. The substituent group(s) is preferably one or more group(s) independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, thiol, hydroxy, nitro, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkylthio, heterocyclylthio, oxo, carboxy and alkoxycarbonyl. The alkyl of the present invention is preferably selected from the group consisting of methyl, ethyl, isopropyl, tert-butyl, haloalkyl, deuterated alkyl, alkoxy-substituted alkyl, hydroxy-substituted alkyl and cyano-substituted alkyl. 
     The term “cycloalkyl” refers to a saturated or partially unsaturated monocyclic or polycyclic hydrocarbon substituent group having 3 to 20 carbon atoms, preferably 3 to 8 carbon atoms, and more preferably 3 to 6 carbon atoms. Non-limiting examples of monocyclic cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cyclohexadienyl, cycloheptyl, cycloheptatrienyl, cyclooctyl and the like. Polycyclic cycloalkyl includes a cycloalkyl having a spiro ring, fused ring or bridged ring. The cycloalkyl is preferably cyclopropyl, cyclobutyl, cyclohexyl, cyclopentyl or cycloheptyl. The cycloalkyl ring can be fused to the ring of aryl, heteroaryl or heterocyclyl, wherein the ring bound to the parent structure is cycloalkyl. Non-limiting examples include indanyl, tetrahydronaphthyl, benzocycloheptyl and the like. The cycloalkyl can be optionally substituted or unsubstituted. When substituted, the substituent group(s) is preferably one or more group(s) independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, thiol, hydroxy, nitro, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkylthio, heterocyclylthio, oxo, carboxy and alkoxycarbonyl. 
     The term “heterocyclyl” refers to a 3 to 20 membered saturated or partially unsaturated monocyclic or polycyclic hydrocarbon group, wherein one or more ring atoms are heteroatoms selected from the group consisting of nitrogen, oxygen and S(O) m  (wherein m is an integer of 0 to 2), but excluding —O—O—, —O—S— or —S—S— in the ring, with the remaining ring atoms being carbon atoms. Preferably, the heterocyclyl has 3 to 12 ring atoms wherein 1 to 4 atoms are heteroatoms; more preferably, 3 to 8 ring atoms; and most preferably 3 to 8 ring atoms. Non-limiting examples of monocyclic heterocyclyl include oxetanyl, pyrrolidinyl, pyrrolidonyl, imidazolidinyl, tetrahydrofuranyl, tetrahydrothienyl, dihydroimidazolyl, dihydrofuranyl, dihydropyrazolyl, dihydropyrrolyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, homopiperazinyl, pyranyl and the like, preferably oxetanyl, pyrrolidonyl, tetrahydrofuranyl, pyrazolidinyl, morpholinyl, piperazinyl and pyranyl. Polycyclic heterocyclyl includes a heterocyclyl having a spiro ring, fused ring or bridged ring. The heterocyclyl having a spiro ring, fused ring or bridged ring is optionally bonded to other group via a single bond, or further bonded to other cycloalkyl, heterocyclyl, aryl and heteroaryl via any two or more atoms on the ring. The heterocyclyl can be optionally substituted or unsubstituted. When substituted, the substituent group(s) is preferably one or more group(s) independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, thiol, hydroxy, nitro, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkylthio, heterocyclylthio, oxo, carboxy and alkoxycarbonyl. 
     The term “aryl” refers to a 6 to 14 membered all-carbon monocyclic ring or polycyclic fused ring (i.e. each ring in the system shares an adjacent pair of carbon atoms with another ring in the system) having a conjugated π-electron system, preferably a 6 to 10 membered aryl, for example, phenyl and naphthyl. The aryl is more preferably phenyl. The aryl ring can be fused to the ring of heteroaryl, heterocyclyl or cycloalkyl, wherein the ring bound to the parent structure is aryl ring. The aryl can be substituted or unsubstituted. When substituted, the substituent group(s) is preferably one or more group(s) independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, thiol, hydroxy, nitro, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkylthio, heterocyclylthio, carboxy and alkoxycarbonyl. 
     The term “heteroaryl” refers to a 5 to 14 membered heteroaromatic system having 1 to 4 heteroatoms selected from the group consisting of oxygen, sulfur and nitrogen. The heteroaryl is preferably a 5 to 10 membered heteroaryl, and more preferably a 5 or 6 membered heteroaryl, for example imidazolyl, furyl, thienyl, thiazolyl, pyrazolyl, oxazolyl, pyrrolyl, triazolyl, tetrazolyl, pyridyl, pyrimidinyl, thiadiazolyl, oxadiazolyl, pyrazinyl and the like, preferably oxazolyl, oxadiazolyl, tetrazolyl, triazolyl, thienyl, imidazolyl, pyridyl, pyrazolyl, pyrimidinyl and thiazolyl, and more preferably oxazolyl, oxadiazolyl, tetrazolyl, triazolyl, thienyl, pyridyl, thiazolyl and pyrimidinyl. The heteroaryl ring can be fused to the ring of aryl, heterocyclyl or cycloalkyl, wherein the ring bound to the parent structure is heteroaryl ring. The heteroaryl can be optionally substituted or unsubstituted. When substituted, the substituent group(s) is preferably one or more group(s) independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, thiol, hydroxy, nitro, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkylthio, heterocyclylthio, carboxy and alkoxycarbonyl. 
     The term “alkoxy” refers to an —O-(alkyl) or an —O-(unsubstituted cycloalkyl) group, wherein the alkyl is as defined above. The alkoxy is preferably an alkoxy having 1 to 8 carbon atoms, more preferably an alkoxy having 1 to 6 carbon atoms, and most preferably an alkoxy having 1 to 3 carbon atoms. Non-limiting examples of alkoxy include methoxy, ethoxy, propoxy, butoxy, cyclopropyloxy, cyclobutyloxy, cyclopentyloxy, cyclohexyloxy. 
     The alkoxy can be optionally substituted or unsubstituted. When substituted, the substituent group(s) is preferably one or more group(s) independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, thiol, hydroxy, nitro, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkylthio, heterocyclylthio, carboxy and alkoxycarbonyl. 
     “Haloalkyl” refers to an alkyl group substituted by one or more halogen(s), wherein the alkyl is as defined above. 
     “Haloalkoxy” refers to an alkoxy group substituted by one or more halogen(s), wherein the alkoxy is as defined above. 
     “Hydroxyalkyl” refers to an alkyl group substituted by hydroxy(s), wherein the alkyl is as defined above. 
     “Alkenyl” refers to a chain alkenyl, also known as alkene group. The alkenyl can be further substituted by other related group, for example alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, thiol, hydroxy, nitro, cyano, cycloalkyl, heterocyclyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkylthio, heterocyclylthio, carboxy or alkoxycarbonyl. 
     “Hydroxy” refers to an —OH group. 
     “Halogen” refers to fluorine, chlorine, bromine or iodine. 
     “Amino” refers to a —NH 2  group. 
     “Cyano” refers to a —CN group. 
     “Nitro” refers to a —NO 2  group. 
     “THF” refers to tetrahydrofuran. 
     “EtOAc” refers to ethyl acetate. 
     “DMSO” refers to dimethyl sulfoxide. 
     “LDA” refers to lithium diisopropylamide. 
     “DMAP” refers to 4-dimethylaminopyridine. 
     “EtMgBr” refers to ethylmagnesium bromide. 
     “HOSu” refers to N-hydroxysuccinimide. 
     “EDCl” refers to 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride. 
     “IPA” refers to isopropanol. 
     “MeOH” refers to methanol. 
     “EtOH” refers to ethanol. 
     “DMF” refers to N,N-dimethylformamide. 
     “DIPEA” refers to N,N-diisopropylethylamine. 
     “HEPES” refers to 4-hydroxyethylpiperazineethanesulfonic acid. 
     Different expressions such as “X is selected from the group consisting of A, B or C”, “X is selected from the group consisting of A, B and C”, “X is A, B or C”, “X is A, B and C” and the like, express the same meaning, that is, X can be any one or more of A, B and C. 
     “Optional” or “optionally” means that the event or circumstance described subsequently can, but need not, occur, and such a description includes the situation in which the event or circumstance does or does not occur. 
     “Substituted” refers to one or more hydrogen atoms in a group, preferably up to 5, and more preferably 1 to 3 hydrogen atoms, independently substituted by a corresponding number of substituents. It goes without saying that the substituents only exist in their possible chemical position. The person skilled in the art is able to determine whether the substitution is possible or impossible by experiments or theory without excessive efforts. For example, the combination of amino or hydroxy having free hydrogen and carbon atoms having unsaturated bonds (such as olefinic) may be unstable. 
     “Stereoisomerism” includes geometric isomerism (cis-trans isomerism), optical isomerism, and conformational isomerism. 
     The hydrogen atom of the present invention can be substituted by its isotope deuterium. Any of the hydrogen atoms in the compounds of the examples of the present invention can also be substituted by deuterium atom(s). 
     A “pharmaceutical composition” refers to a mixture of one or more of the compounds according to the present invention or physiologically/pharmaceutically acceptable salts or prodrugs thereof with other chemical components, and other components such as physiologically/pharmaceutically acceptable carriers and excipients. The purpose of the pharmaceutical composition is to facilitate administration of a compound to an organism, which is conducive to the absorption of the active ingredient so as to exert biological activity. 
     A “pharmaceutically acceptable salt” refers to a salt of the compound of the present invention, which is safe and effective in mammals and has the desired biological activity. 
     X-ray powder diffraction (XRPD) pattern refers to the experimentally observed diffraction pattern or the parameters derived from it, and the X-ray powder diffraction pattern is characterized by peak position (abscissa) and peak intensity (ordinate). Those skilled in the art will appreciate that the experimental error therein depends on the conditions of the instrument, the preparation of the sample, and the purity of the sample. In particular, it is well known to those skilled in the art that X-ray diffraction pattern generally varies with the conditions of the instrument. Those skilled in the art will appreciate that suitable error tolerances for XRPD may be: 20±0.5°, 20±0.4°, 20±0.3°, 20±0.2°. In particular, it is important to point out that the relative intensity in X-ray diffraction pattern may vary with experimental conditions, so the order of peak intensity cannot be used as the sole or decisive factor. In addition, due to the influence of experimental factors such as the height of the sample, the overall deviation of the peak angle will occur, and a certain deviation is usually allowed. Therefore, those skilled in the art can understand that any crystal form having the same or similar characteristic peaks as the pattern of the present invention falls within the scope of the present invention. 
     “TGA” refers to thermogravimetric analysis (TGA) test. 
     “DSC” refers to differential scanning calorimetry (DSC) test. 
     “HPLC” refers to high performance liquid chromatography (HPLC) test. 
     “PK” refers to pharmacokinetic (PK) test. 
     The present invention will be further described with reference to the following examples, but the examples should not be considered as limiting the scope of the present invention. 
     I. Preparation of the Compounds 
     The structures of the compounds of the present invention were identified by nuclear magnetic resonance (NMR) and/or liquid chromatography-mass spectrometry (LC-MS). NMR shifts (δ) are given in parts per million (ppm). NMR was determined by a Bruker AVANCE-400 machine. The solvents for determination were deuterated-dimethyl sulfoxide (DMSO-d 6 ), deuterated-methanol (CD 3 OD) and deuterated-chloroform (CDCl 3 ), and the internal standard was tetramethylsilane (TMS). 
     Liquid chromatography-mass spectrometry (LC-MS) was determined on an Agilent 1200 Infinity Series mass spectrometer. High performance liquid chromatography (HPLC) was determined on an Agilent 1200DAD high pressure liquid chromatograph (Sunfire C18 150×4.6 mm chromatographic column), and a Waters 2695-2996 high pressure liquid chromatograph (Gimini C18 150×4.6 mm chromatographic column). 
     Yantai Huanghai HSGF254 or Qingdao GF254 silica gel plate was used as the thin-layer silica gel chromatography (TLC) plate. The dimension of the silica gel plate used in TLC was 0.15 mm to 0.2 mm, and the dimension of the silica gel plate used in product purification was 0.4 mm to 0.5 mm. Yantai Huanghai 200 to 300 mesh silica gel was generally used as a carrier for column chromatography. 
     The raw materials used in the examples of the present invention are known and commercially available, or can be synthesized by adopting or according to known methods in the art. 
     Unless otherwise stated, all reactions of the present invention were carried out under continuous magnetic stirring under a dry nitrogen or argon atmosphere, the solvent was dry, and the reaction temperature was in degrees celsius. 
     Example 1 
     Preparation of (S)-1′-(6-amino-5-((2-amino-3-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 2-ethylhexyl 3-((2-amino-3-chloropyridin-4-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     In a microwave reaction tube, 3-chloro-4-iodopyridin-2-amine (800 mg, 3.15 mmol) and 2-ethylhexyl 3-mercaptopropanoate (687 mg, 3.15 mmol) were dissolved in 1,4-dioxane (7 mL), followed by the addition of DIPEA (813 mg, 6.3 mmol), palladium acetate (35 mg, 0.16 mmol) and Xantphos (109 mg, 0.19 mmol). The reaction solution was bubbled with nitrogen for 3 minutes, heated under microwave to 100° C. and reacted for 1 hour. The reaction solution was cooled, diluted with 20 mL of ethyl acetate, and filtered through diatomaceous earth to remove insoluble substance. The filtrate was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography (15 to 25% ethyl acetate/petroleum ether) to obtain the product (1.03 g, yield: 95%) as a brown solid. 
     MS m/z (ESI): 345.1 [M+H] + . 
     Step 2: Preparation of 2-ethylhexyl 3-((2-amino-3-cyclopropylpyridin-4-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     In a sealed tube, 2-ethylhexyl 3-((2-amino-3-chloropyridin-4-yl)thio)propanoate (500 mg, 1.45 mmol) was dissolved in a mixed solvent of toluene and water (toluene/water-10 mL/1 mL), followed by the addition of potassium cyclopropyl trifluoroborate (280 mg, 1.89 mmol), n-butylbis(1-adamantyl)phosphine (52 mg, 0.145 mmol), palladium acetate (16 mg, 0.073 mmol) and cesium carbonate (1.41 g, 4.35 mmol). The reaction solution was bubbled with nitrogen for 3 minutes, heated to 100° C. and reacted for 5 hours. The reaction solution was cooled to room temperature, and then 20 mL of saturated NH 4 Cl solution was added. 
     The reaction solution was extracted with ethyl acetate (20 mL×3). The ethyl acetate layer was washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, and purified by column chromatography (50% ethyl acetate/petroleum ether) to obtain the product (220 mg, yield: 43%) as a brown oil. 
     MS m/z (ESI): 350.1 [M+H] + . 
     Step 3: Preparation of potassium 2-amino-3-cyclopropylpyridine-4-thiolate 
     
       
         
         
             
             
         
       
     
     2-Ethylhexyl 3-((2-amino-3-cyclopropylpyridin-4-yl)thio)propanoate (220 mg, 0.63 mmol) was dissolved in 10 mL of ethanol. Potassium tert-butoxide (74 mg, 0.66 mmol) was added, and the reaction solution was stirred at room temperature for 1 hour. The reaction solution was concentrated to dryness, and the resulting residue was used directly in the next step. 
     MS m/z (ESI): 165.1 [M−H] − . 
     Step 4: Preparation of 3-((2-amino-3-cyclopropylpyridin-4-yl)thio)-6-chloropyrazin-2-amine 
     
       
         
         
             
             
         
       
     
     In a microwave reaction tube, potassium 2-amino-3-cyclopropylpyridine-4-thiolate (128 mg, 0.63 mmol) and 2-amino-3-bromo-6-chloropyrazine (149 mg, 0.72 mmol) were dissolved in 7 mL of 1,4-dioxane, followed by the addition of tris(dibenzylideneacetone)dipalladium (33 mg, 0.036 mmol), Xantphos (42 mg, 0.072 mmol) and DIPEA (279 mg, 2.16 mmol). The reaction solution was bubbled with nitrogen for 3 minutes, heated under microwave to 110° C. and reacted for 1 hour. The reaction solution was cooled to room temperature, diluted with 20 mL of ethyl acetate, and filtered through diatomaceous earth. The filtrate was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography (50 to 70% ethyl acetate/petroleum ether) to obtain the product (50 mg, yield: 24%) as a brown solid. 
     MS m/z (ESI): 294.1 [M+H] + . 
     Step 5: Preparation of (R)—N—((S)-1′-(6-amino-5-((2-amino-3-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     (S)-1-(((R)-tert-butylsulfinyl)amino)-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate (69 mg, 0.17 mmol) was dissolved in 3 mL of dichloromethane. 1 mL of trifluoroacetic acid was added, and the reaction solution was reacted at room temperature for 1 hour. The reaction solution was concentrated to dryness, and dissolved in 5 mL of DMF. Potassium carbonate (352 mg, 2.55 mmol) and 3-((2-amino-3-cyclopropylpyridin-4-yl)thio)-6-chloropyrazin-2-amine (50 mg, 0.17 mmol) were added, and the reaction solution was heated to 100° C. under a nitrogen atmosphere and reacted for 12 hours. The reaction solution was cooled, and then 20 mL of water was added. The reaction solution was extracted with ethyl acetate (20 mL×3). The ethyl acetate layer was washed with saturated sodium chloride solution (20 mL×3), dried over anhydrous sodium sulfate, and purified by column chromatography (75 to 80% ethyl acetate/petroleum ether) to obtain the product (20 mg, yield: 21%) as an oil. 
     MS m/z (ESI): 564.1 [M+H] + . 
     Step 6: Preparation of (S)-1′-(6-amino-5-((2-amino-3-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-1′-(6-Amino-5-((2-amino-3-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (20 mg, 0.035 mmol) was dissolved in 5 mL of dichloromethane. 1 mL of 4M HCl in dioxane was added, and the reaction solution was reacted at room temperature for 1 hour. The reaction solution was concentrated to dryness and dissolved in 5 mL of methanol, and the pH was adjusted to alkaline with 7M NH 3  in methanol. The reaction solution was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography (0 to 10% MeOH in DCM). The resulting crude product was purified by thin layer chromatography (dichloromethane:methanol=10:1) to obtain the product (9.0 mg, yield: 56%) as a brown solid. 
       1 H NMR (400 MHz, Methanol-d 4 ) δ 7.90 (d, J=5.4 Hz, 1H), 7.61 (s, 1H), 7.40-7.33 (m, 1H), 7.27-7.16 (m, 3H), 6.57 (d, J=5.4 Hz, 1H), 4.28 (d, J=13.6 Hz, 2H), 3.96 (s, 1H), 3.30-3.19 (m, 2H), 3.15 (d, J=15.7 Hz, 1H), 2.81 (d, J=15.7 Hz, 1H), 1.90-1.78 (m, 2H), 1.82-1.70 (m, 1H), 1.58 (d, J=13.5 Hz, 1H), 1.43 (d, J=13.1 Hz, 1H), 1.26-1.15 (m, 2H), 0.94-0.85 (m, 2H). 
     MS m/z (ESI): 460.1 [M+H] + . 
     Example 2 
     Preparation of (S)-1′-(6-amino-5-((2-chloro-3-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 2 was prepared by referring to the experimental scheme of Example 1. 
       1 H NMR (400 MHz, Methanol-d 4 ) δ 7.88 (d, J=5.5 Hz, 1H), 7.60 (s, 1H), 7.38-7.30 (m, 1H), 7.25-7.15 (m, 3H), 6.54 (d, J=5.5 Hz, 1H), 4.26 (d, J=13.7 Hz, 2H), 3.95 (s, 1H), 3.28-3.17 (m, 2H), 3.13 (d, J=15.6 Hz, 1H), 2.79 (d, J=15.6 Hz, 1H), 1.88-1.74 (m, 2H), 1.80-1.70 (m, 1H), 1.56 (d, J=13.7 Hz, 1H), 1.43 (d, J=13.1 Hz, 1H), 1.24-1.13 (m, 2H), 0.93-0.83 (m, 2H). 
     MS m/z (ESI): 479.1 [M+H] + , 481.1 [M+2+H] + . 
     Example 3 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 2-ethylhexyl 3-((2,3-dichloropyridin-4-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     In a microwave reaction tube, 2,3-dichloro-4-iodopyridine (1.0 g, 3.65 mmol) and 2-ethylhexyl 3-mercaptopropanoate (0.88 g, 4.03 mmol) were dissolved in 1,4-dioxane (7 mL), followed by the addition of DIPEA (0.95 g, 7.34 mmol), palladium acetate (41 mg, 0.18 mmol) and Xantphos (127 mg, 0.22 mmol). The reaction solution was bubbled with nitrogen for 3 minutes, heated under microwave to 100° C. and reacted for 1 hour. The reaction solution was cooled, diluted with 20 mL of ethyl acetate, and filtered through diatomaceous earth to remove insoluble substance. The filtrate was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography (10 to 15% ethyl acetate/petroleum ether) to obtain a brown oil (1.29 g, yield: 97%). 
     MS m/z (ESI): 364.1 [M+H] + . 
     Step 2: Preparation of 2-ethylhexyl 3-((3-chloro-2-cyclopropylpyridin-4-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     In a sealed tube, 2-ethylhexyl 3-((2,3-dichloropyridin-4-yl)thio)propanoate (1.29 g, 3.54 mmol) was dissolved in a mixed solvent of toluene and water (toluene/water=20 mL/2 mL), followed by the addition of potassium cyclopropyl trifluoroborate (624 mg, 4.25 mmol), n-butylbis(1-adamantyl)phosphine (95 mg, 0.267 mmol), palladium acetate (40 mg, 0.178 mmol) and cesium carbonate (3.43 g, 3.54 mmol). The reaction solution was bubbled with nitrogen for 3 minutes, heated to 100° C. and reacted for 5 hours. The reaction solution was cooled to room temperature, and then 20 mL of saturated NH 4 Cl solution was added. The reaction solution was extracted with ethyl acetate (20 mL×3). The ethyl acetate layer was washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, and purified by column chromatography (5 to 8% ethyl acetate/petroleum ether) to obtain a yellow oil (600 mg, yield: 45%). 
     MS m/z (ESI): 370.1 [M+H] + . 
     Step 3: Preparation of potassium 3-chloro-2-cyclopropylpyridine-4-thiolate 
     
       
         
         
             
             
         
       
     
     2-Ethylhexyl 3-((3-chloro-2-cyclopropylpyridin-4-yl)thio)propanoate (600 mg, 1.62 mmol) was dissolved in 15 mL of ethanol. Potassium tert-butoxide (190 mg, 1.70 mmol) was added, and the reaction solution was stirred at room temperature for 1 hour. The reaction solution was concentrated to dryness, and the resulting residue was used directly in the next step. 
     MS m/z (ESI): 186.1 [M+H] + . 
     Step 4: Preparation of 6-chloro-3-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-amine 
     
       
         
         
             
             
         
       
     
     In a microwave reaction tube, potassium 3-chloro-2-cyclopropylpyridine-4-thiolate (120 mg, 0.54 mmol) and 2-amino-3-bromo-6-chloropyrazine (112 mg, 0.54 mmol) were dissolved in 5 mL of 1,4-dioxane, followed by the addition of tris(dibenzylideneacetone)dipalladium (25 mg, 0.027 mmol), Xantphos (31 mg, 0.054 mmol) and DIPEA (209 mg, 1.62 mmol). The reaction solution was bubbled with nitrogen for 3 minutes, heated under microwave to 110° C. and reacted for 1 hour. The reaction solution was cooled to room temperature, diluted with 20 mL of ethyl acetate, and filtered through diatomaceous earth. The filtrate was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography (10 to 20% ethyl acetate/petroleum ether) to obtain the product (135 mg, yield: 80%) as an off-white solid. 
     MS m/z (ESI): 313.1 [M+H] + . 
     Step 5: Preparation of tert-butyl 1-oxo-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     1-Indenone (5.14 g, 38.9 mmol) and tert-butyl N,N-bis(2-chloroethyl)carbamate (9.42 g, 38.9 mmol) were dissolved in 100 mL of DMF. 60% Sodium hydride (3.89 g, 97.3 mmol) was added in batches in an ice bath. The reaction solution was heated to 60° C. in an oil bath under a nitrogen atmosphere and reacted overnight. The reaction solution was cooled, and then 250 mL of saturated sodium chloride solution was added. The reaction solution was extracted with ethyl acetate (150 mL×2). The ethyl acetate layer was washed with saturated sodium chloride solution (100 mL×3), dried over anhydrous MgSO 4 , and purified by column chromatography (10 to 20% ethyl acetate/petroleum ether) to obtain the crude product (2.80 g, yield: 23%) as a brown oil. 
     MS m/z (ESI): 202.1 [M-Boc+H] + . 
     Step 6: Preparation of tert-butyl (R,E)-1-((tert-butylsulfinyl)imino)-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     Tetraethyl titanate (40 mL) was heated to 90° C. Tert-butyl 1-oxo-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate (2.80 g, 9.27 mmol) and (R)-(+)-tert-butylsulfinamide (3.36 g, 27.8 mmol) were added, and the reaction solution was reacted for 24 hours at 90° C. under a nitrogen atmosphere. After completion of the reaction, the reaction solution was poured into 400 mL of ethyl acetate. 400 mL of saturated sodium chloride solution was slowly added under stirring, and the reaction solution was stirred at room temperature for 20 minutes. The reaction solution was filtered through diatomaceous earth to remove the precipitated solid. After the resulting filtrate was separated into two layers, the ethyl acetate layer was dried over anhydrous magnesium sulfate, and purified by column chromatography (20 to 30% ethyl acetate/petroleum ether) to obtain the product (2.20 g, yield: 59%) as a brown oil. 
     MS m/z (ESI): 405.1 [M+H] + . 
     Step 7: Preparation of tert-butyl (S)-1-(((R)-tert-butylsulfinyl)amino)-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     Tert-butyl (R,E)-1-((tert-butyl sulfinyl)imino)-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate (2.20 g, 5.44 mmol) was dissolved in 30 mL of tetrahydrofuran. The solution was cooled to −78° C., and sodium borohydride (308 mg, 8.16 mmol) was added in batches at this temperature. The reaction solution was gradually warmed up to room temperature under a nitrogen atmosphere and stirred overnight. 200 mL of saturated sodium chloride solution was added, and the reaction solution was extracted with ethyl acetate (100 mL×2). The ethyl acetate layer was washed with saturated sodium chloride solution (100 mL), dried over anhydrous sodium sulfate, and purified by column chromatography (20 to 35% ethyl acetate/petroleum ether) to obtain a brown foamy solid (1.21 g, yield: 54%). 
     MS m/z (ESI): 407.1 [M+H] + . 
     Step 8: Preparation of (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     Tert-butyl (S)-1-(((R)-tert-butyl sulfinyl)amino)-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate (100 mg, 0.25 mmol) was dissolved in 3 mL of dichloromethane. 1 mL of trifluoroacetic acid was added, and the reaction solution was reacted at room temperature for 1 hour. The reaction solution was concentrated to dryness, and dissolved in 5 mL of DMF. Potassium carbonate (517 mg, 3.75 mmol) and 6-chloro-3-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-amine (78 mg, 0.25 mmol) were added, and the reaction solution was heated to 100° C. under a nitrogen atmosphere and reacted for 12 hours. The reaction solution was cooled, and then 20 mL of water was added. The reaction solution was extracted with ethyl acetate (20 mL×3). The ethyl acetate layer was washed with saturated sodium chloride solution (20 mL×3), dried over anhydrous sodium sulfate, and purified by column chromatography (50% ethyl acetate/petroleum ether) to obtain the product (26 mg, yield: 18%) as an oil. 
     MS m/z (ESI): 583.1 [M+H] + . 
     Step 9: Preparation of (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-1′-(6-Amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (26 mg, 0.044 mmol) was dissolved in 5 mL of dichloromethane. 1 mL of 4M HCl in dioxane was added, and the reaction solution was reacted at room temperature for 1 hour. The reaction solution was concentrated to dryness and dissolved in 5 mL of methanol, and the pH was adjusted to alkaline with 7M NH 3  in methanol. The reaction solution was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography (5 to 8% MeOH in DCM) to obtain the product (12 mg, yield: 57%) as a light yellow solid. 
       1 H NMR (400 MHz, Chloroform-d) δ 8.03 (d, J=5.2 Hz, 1H), 7.67 (s, 1H), 7.44-7.37 (m, 1H), 7.24 (dd, J=6.4, 3.9 Hz, 3H), 6.39 (d, J=5.2 Hz, 1H), 4.85 (s, 2H), 4.21-4.13 (m, 2H), 4.07 (s, 1H), 3.23-3.21 (m, 2H), 3.13 (d, J=15.8 Hz, 1H), 2.79 (d, J=15.7 Hz, 1H), 2.57-2.48 (m, 1H), 1.88-1.73 (m, 2H), 1.63-1.56 (m, 1H), 1.50-1.42 (m, 1H), 1.09-1.00 (m, 4H). 
     MS m/z (ESI): 479.1 [M+H] + . 
     Example 4 
     Preparation of (2R)-1′-(6-amino-5-((2-amino-3-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)spiro[bicyclo[3.1.0]hexane-3,4′-piperidin]-2-amine 
     
       
         
         
             
             
         
       
     
       1 H NMR (400 MHz, Methanol-d 4 ) δ 7.73-7.67 (m, 1H), 7.65-7.60 (m, 1H), 6.08-6.01 (m, 1H), 4.53-4.41 (m, 2H), 3.63-3.51 (m, 2H), 3.14 (p, J=9.6 Hz, 1H), 2.41-2.33 (m, 1H), 1.56-1.40 (m, 3H), 1.44-1.35 (m, 1H), 1.39-1.19 (m, 4H), 0.89-0.81 (m, 2H), 0.76-0.60 (m, 2H), 0.44-0.28 (m, 2H). 
     MS m/z (ESI): 424.1 [M+H] + . 
     The compound of Example 4 was prepared by referring to the experimental scheme of Example 1. 
     Example 5 
     Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-bromopyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 2-ethylhexyl 3-((3-amino-5-chloropyrazin-2-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     3-Bromo-6-chloropyrazin-2-amine (4 g, 20 mmol), 2-ethylhexyl 3-mercaptopropanoate (5.2 g, 24 mmol), tris(dibenzylideneacetone)dipalladium (916 mg, 1 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (1.16 g, 2 mmol) and N,N-diisopropylethylamine (5.12 g, 40 mmol) were stirred in dioxane (35 mL) at 100° C. for 18 hours. The reaction solution was filtered, and the filter cake was washed with ethyl acetate (30 mL) twice. The filtrate was concentrated, and purified by column chromatography [eluent: petroleum ether˜petroleum ether/ethyl acetate (90:10)] to obtain 2-ethylhexyl 3-((3-amino-5-chloropyrazin-2-yl)thio)propanoate (5.5 g, yield: 82%) as a brown oil. 
     MS m/z (ESI): 146.1 [M+H] + , 148.1 [M+2+H] + . 
     Step 2: Preparation of 3-amino-5-chloropyrazine-2-thiol 
     
       
         
         
             
             
         
       
     
     Potassium tert-butoxide (2.7 g, 23.9 mmol) was added to a solution of 2-ethylhexyl 3-((3-amino-5-chloropyrazin-2-yl)thio)propanoate (5.5 g, 15.9 mmol) in ethanol (100 mL). After completion of the addition, the reaction solution was stirred at room temperature for 3 hours. The reaction solution was concentrated by rotary evaporation to remove about 50 mL of ethanol. The remaining reaction solution was poured into aqueous ammonium chloride solution (100 mL), and extracted with ethyl acetate (100 mL) twice and dichloromethane (100 mL) twice. The organic phase was concentrated, and purified by column chromatography [eluent: dichloromethane˜dichloromethane/methanol (95:5)] to obtain 3-amino-5-chloropyrazine-2-thiol (1.8 g, yield: 70%) as a dark green solid. 
     MS m/z (ESI): 162.0[M+H] + , 164.0 [M+2+H] +   
     Step 3: Preparation of 3-((2-amino-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine 
     
       
         
         
             
             
         
       
     
     3-Amino-5-chloropyrazine-2-thiol (500 mg, 3.1 mmol), 3-chloro-4-iodopyridin-2-amine (789 mg, 3.1 mmol), tris(dibenzylideneacetone)dipalladium (142 mg, 0.16 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (179 mg, 0.31 mmol) and N,N-diisopropylethylamine (1.2 g, 9.3 mmol) were stirred in dioxane (10 mL) at 130° C. under microwave for 1 hour. The reaction solution was concentrated, and purified by column chromatography [eluent: dichloromethane˜dichloromethane/methanol (99:1)] to obtain 1 g of a crude product. The crude product was pulped in ethanol (5 mL), and filtered to obtain 3-((2-amino-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine (580 mg, yield: 65%) as a grey solid. 
     MS m/z (ESI): 288.0 [M+H] + , 290.0 [M+2+H] + . 
     Step 4: Preparation of N—((S)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     Trifluoroacetic acid (1 mL) was added to a solution of tert-butyl (1 S)-1-((tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate (150 mg, 0.37 mmol) in dichloromethane (3 mL). After completion of the addition, the reaction solution was stirred at room temperature for 2 hours. The reaction solution was concentrated to obtain N—((S)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide hydrochloride (150 mg, yield: 100%) as a light yellow solid. 
     MS m/z (ESI): 307.2 [M+H] + . 
     [α] 20   D =1.773. 
     Step 5: Preparation of N—((S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     N—((S)-1,3-Dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide hydrochloride (150 mg, 0.37 mmol), 3-((2-amino-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine (100 mg, 0.35 mmol) and potassium carbonate (335 mg, 2.43 mmol) were stirred in N,N-dimethylformamide (4 mL) at 100° C. for 18 hours. The reaction solution was concentrated, and purified by column chromatography [eluent: dichloromethane˜dichloromethane/methanol (97:3)] to obtain N—((S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (100 mg, yield: 52%) as a purple solid. 
     MS m/z (ESI): 558.1 [M+H] + , 560.2 [M+2+H] + . 
     Step 6: Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-bromopyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     N—((S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (100 mg, 0.18 mmol) and N-bromosuccinimide (64 mg, 0.36 mmol) were stirred in N,N-dimethylformamide (1 mL) at room temperature for 18 hours. The reaction solution was concentrated to obtain a crude product, which was purified by column chromatography [eluent: dichloromethane˜dichloromethane/methanol (95:5)] to obtain (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-bromopyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine (80 mg, yield: 84%) as a khaki solid. 
       1 H NMR (400 MHz, DMSO-d6) δ 7.72-7.64 (m, 1H), 7.36 (d, J=4 Hz, 1H), 7.27-7.16 (m, 3H), 6.58 (s, 2H), 6.33 (s, 2H), 5.80 (d, J=4 Hz, 1H), 3.99 (s, 1H), 3.94-3.83 (m, 2H), 3.17 (d, J=4 Hz, 1H), 3.14-2.98 (m, 3H), 2.77-2.64 (m, 1H), 1.97-1.74 (m, 2H), 1.55 (d, J=12 Hz, 1H). 
     MS m/z (ESI): 532.0 [M+H] + , 534.0 [M+2+H] + . 
     Example 6 
     Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-bromopyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine (30 mg, 0.056 mmol), trimethylboroxine (789 mg, 3.1 mmol), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium (4 mg, 0.0056 mmol) and potassium carbonate (15 mg, 0.11 mmol) were stirred in N,N-dimethylformamide (10 mL) at 130° C. under microwave for 1 hour. The reaction solution was concentrated and purified by high performance liquid chromatography to obtain (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-amine (0.7 mg, yield: 3%) as a grey solid. 
       1 H NMR (400 MHz, DMSO) δ 7.73-7.62 (m, 1H), 7.35 (d, J=4 Hz, 1H), 7.28-7.16 (m, 3H), 6.57 (s, 2H), 6.32 (s, 2H), 5.78 (d, J=4 Hz, 1H), 3.97 (s, 1H), 3.94-3.81 (m, 2H), 3.15 (d, J=4 Hz, 1H), 3.12-2.95 (m, 3H), 2.75-2.64 (m, 1H), 2.41 (s, 3H), 1.99-1.75 (m, 2H), 1.58 (d, J=12 Hz, 1H). 
     MS m/z (ESI): 468.1 [M+H] + , 470.1 [M+2+H] + . 
     Example 7 
     Preparation of (S)-1′-(5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 2-chloro-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazine 
     
       
         
         
             
             
         
       
     
     In a microwave reaction tube, potassium 3-chloro-2-cyclopropylpyridine-4-thiolate (120 mg, 0.54 mmol) and 2-bromo-5-chloropyrazine (104 mg, 0.54 mmol) were dissolved in 5 mL of 1,4-dioxane, followed by the addition of tris(dibenzylideneacetone)dipalladium (25 mg, 0.027 mmol), Xantphos (31 mg, 0.054 mmol) and DIPEA (209 mg, 1.62 mmol). 
     The reaction solution was bubbled with nitrogen for 3 minutes, heated under microwave to 110° C. and reacted for 1 hour. The reaction solution was cooled to room temperature, diluted with 20 mL of ethyl acetate, and filtered through diatomaceous earth. The filtrate was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography (10 to 20% ethyl acetate/petroleum ether) to obtain a light yellow oil (120 mg, yield: 74%). 
     MS m/z (ESI): 298.0 [M+H] + . 
     Step 2: Preparation of (R)—N—((S)-1′-(5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     Tert-butyl (S)-1-(((R)-tert-butylsulfinyl)amino)-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate (81 mg, 0.20 mmol) was dissolved in 3 mL of dichloromethane. 1 mL of trifluoroacetic acid was added, and the reaction solution was reacted at room temperature for 1 hour. The reaction solution was concentrated to dryness, and dissolved in 5 mL of DMF. Potassium carbonate (373 mg, 2.70 mmol) and 2-chloro-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazine (55 mg, 0.18 mmol) were added, and the reaction solution was heated to 100° C. under a nitrogen atmosphere and reacted for 12 hours. The reaction solution was cooled, and then 20 mL of water was added. The reaction solution was extracted with ethyl acetate (20 mL×3). The ethyl acetate layer was washed with saturated sodium chloride solution (20 mL×3), dried over anhydrous sodium sulfate, and purified by column chromatography (50% ethyl acetate/petroleum ether) to obtain the product (48 mg, yield: 47%) as an oil. 
     MS m/z (ESI): 568.1 [M+H] + . 
     Step 3: Preparation of (S)-1′-(5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-1′-(5-((3-Chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (48 mg, 0.085 mmol) was dissolved in 5 mL of dichloromethane. 1 mL of 4M HCl in dioxane was added, and the reaction solution was reacted at room temperature for 1 hour. The reaction solution was concentrated to dryness and dissolved in 5 mL of methanol, and the pH was adjusted to alkaline with 7M NH 3  in methanol. The reaction solution was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography (0 to 10% MeOH in DCM) to obtain the product (23 mg, yield: 58%) as a light yellow solid. 
       1 H NMR (400 MHz, Methanol-d 4 ) δ 8.36 (s, 1H), 8.30 (s, 1H), 7.98 (d, J=5.3 Hz, 1H), 7.42 (d, J=6.8 Hz, 1H), 7.32-7.21 (m, 3H), 6.43 (d, J=5.3 Hz, 1H), 4.44-4.29 (m, 2H), 4.14 (s, 1H), 3.41-3.30 (m, 2H), 3.19 (d, J=15.9 Hz, 1H), 2.96 (d, J=15.9 Hz, 1H), 2.60-2.49 (m, 1H), 1.90-1.76 (m, 2H), 1.67-1.54 (m, 2H), 1.09-0.99 (m, 4H). 
     MS m/z (ESI): 464.1 [M+H] + . 
     Example 8 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of tert-butyl (5-bromo-1,3,4-thiadiazol-2-yl)carbamate 
     
       
         
         
             
             
         
       
     
     Ethyl 2-chlorothiazole-4-carboxylate (9.0 g, 46.97 mmol) was dissolved in 150 mL of methanol and stirred at room temperature. NaBH 4  (7.1 g, added in three batches) was added, and the reaction solution was stirred until the starting materials disappeared. The reaction solution was slowly added to brine under stirring to quench the reaction. The reaction solution was concentrated to remove the solvent, and extracted with ethyl acetate (200 mL) three times. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product, which was purified by column chromatography (ethyl acetate/petroleum ether=3:1) to obtain the target product (2-chlorothiazol-4-yl)methanol (4.2 g, yield: 60%) as a colorless oily liquid. 
       1 H NMR (400 MHz, CDCl 3 ) δ 7.11 (s, 1H), 4.71 (s, 2H), 2.22 (s, 1H). 
     MS m/z (ESI): 150.0 [M+H] + , 152.0 [M+2+H] + . 
     Step 2: Preparation of (2-chlorothiazol-4-yl)methyl methanesulfonate 
     
       
         
         
             
             
         
       
     
     (2-Chlorothiazol-4-yl)methanol (2.6 g, 17.4 mmol) was dissolved in 40 mL of dichloromethane. Methanesulfonyl chloride (1.6 mL, 20.88 mmol, d=1.48 g/mL) was added dropwise under a nitrogen atmosphere in an ice water bath, and the reaction solution was stirred in the ice water bath for 30 minutes. After completion of the reaction, the reaction solution was added dropwise to saturated sodium chloride solution (100 mL), and extracted with dichloromethane (100 mL) three times. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain the target product (2-chlorothiazol-4-yl)methyl methanesulfonate (3.9 g, yield: 97%) as a white solid. 
       1 H NMR (400 MHz, CDCl 3 ) δ 7.35 (s, 1H), 5.25 (s, 2H), 3.07 (s, 3H). 
     Step 3: Preparation of 1-(tert-butyl) 4-ethyl 4-((2-chlorothiazol-4-yl)methyl)piperidine-1,4-dicarboxylate 
     
       
         
         
             
             
         
       
     
     1-(Tert-butyl) 4-ethyl piperidine-1,4-dicarboxylate (4.8 g, 18.59 mmol) was dissolved in THE (40 mL). The solution was cooled to −60° C. under a nitrogen atmosphere, and then lithium diisopropylamide (12.7 mL, 25.35 mmol) was added dropwise. After completion of the addition, the reaction solution was stirred at −60° C. to −50° C. for 30 minutes. A solution of (2-chlorothiazol-4-yl)methyl methanesulfonate in THE (15 mL) was added dropwise. After completion of the addition, the reaction solution was stirred at −60° C. for 30 minutes, then slowly warmed up to room temperature and stirred for 2 hours. After completion of the reaction, the reaction solution was added dropwise to saturated brine (150 mL), and extracted with ethyl acetate (100 mL) three times. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product, which was purified by column chromatography (ethyl acetate/petroleum ether-3:1) to obtain the target product 1-(tert-butyl) 4-ethyl 4-((2-chlorothiazol-4-yl)methyl)piperidine-1,4-dicarboxylate (3.7 g, yield: 56%) as a yellow liquid. 
       1 H NMR (400 MHz, CDCl 3 ) δ 6.79 (s, 1H), 4.14 (dd, J=7.1, 3.1 Hz, 2H), 3.91-3.85 (m, 2H), 2.92 (s, 2H), 2.86 (dd, J=16.3, 13.6 Hz, 2H), 2.10 (d, J=13.5 Hz, 2H), 1.53 (s, 2H), 1.44 (s, 9H), 1.25-1.22 (m, 3H). 
     MS m/z (ESI): 289 [M−100+H] + . 
     Step 4: Preparation of tert-butyl 2-chloro-6-oxo-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     1-(Tert-butyl) 4-ethyl 4-((2-chlorothiazol-4-yl)methyl)piperidine-1,4-dicarboxylate (4.1 g, 10.6 mmol) was dissolved in THF (40 mL). The solution was cooled to −70° C. under a nitrogen atmosphere, and then lithium diisopropylamide (13.4 mL, 26.5 mmol) was added dropwise. After completion of the addition, the reaction solution was stirred at −70° C. for 30 minutes. After completion of the reaction, the reaction solution was added dropwise to saturated brine (150 mL), and extracted with ethyl acetate (100 mL) three times. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product, which was purified by column chromatography (ethyl acetate/petroleum ether=3:1) to obtain the target product tert-butyl 2-chloro-6-oxo-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate (1.2 g, yield: 33%) as a yellow solid. 
       1 H NMR (400 MHz, CDCl 3 ) δ 4.15 (s, 4H), 3.05 (s, 2H), 2.06-1.93 (m, 2H), 1.60 (d, J=12.7 Hz, 2H), 1.48 (s, 9H). 
     MS m/z (ESI): 243 [M−100+H] + . 
     Step 5: Preparation of tert-butyl (R,Z)-6-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     Tert-butyl 2-chloro-6-oxo-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate (1.2 g, 3.5 mmol) was dissolved in THE (10 mL). Ti(OEt) 4  (10 mL) was added, and the reaction solution was heated to 95° C. under a nitrogen atmosphere and stirred for 10 hours. The reaction solution was cooled to room temperature, and diluted with ethyl acetate (200 mL). Water (20 mL) was added under stirring, and the solution was stirred until cloudy. Anhydrous sodium sulfate was added thereto until the solid in the mixture was sandy. The mixture was filtered, and the filtrate was concentrated to obtain a crude product, which was purified by column chromatography (PE/EA=5:1) to obtain the target product tert-butyl (R,Z)-6-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate (1.1 g, yield: 75%) as a yellow solid. 
       1 H NMR (400 MHz, CDCl 3 ) δ 4.13 (dd, J=22.7, 15.6 Hz, 4H), 2.92 (d, J=2.9 Hz, 2H), 2.05 (s, 2H), 1.57 (s, 2H), 1.48 (s, 9H), 1.29-1.22 (m, 9H). 
     MS m/z (ESI): 346 [M−100+H] + . 
     Step 6: Preparation of tert-butyl (S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate and tert-butyl (S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     Tert-butyl (R,Z)-6-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate (600 mg, 1.35 mmol) was dissolved in anhydrous THF (10 mL) in a 50 mL three-neck flask. The solution was cooled to −70° C. under a nitrogen atmosphere, and then BH 3 /THF (4.0 mL, D=1 M) was added dropwise. After completion of the addition, the reaction solution was slowly warmed to room temperature and stirred for 10 hours. The reaction solution was added dropwise to water, and saturated sodium bicarbonate solution was added to adjust the pH to 9. The reaction solution was extracted with ethyl acetate (100 mL) three times. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product, which was purified by column chromatography (PE/EA=1:1) to obtain the target products tert-butyl (S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate (112 mg, yield: 20%) as a yellow liquid and tert-butyl (S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate (56 mg, yield: 9%) as a yellow liquid. 
     MS m/z (ESI): 414.0 [M+H] + ; 
     MS m/z (ESI): 448.1 [M+H] + , 450.1 [M+H+2] + . 
     Step 7: Preparation of (R)—N—((S)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     Tert-butyl (S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate (112 mg, 0.27 mmol) was dissolved in anhydrous CH 2 Cl 2  (5 mL). Trifluoroacetic acid (1.0 mL) was added, and the reaction solution was stirred at room temperature for 2 hours. The reaction solution was concentrated to obtain the crude product (R)—N—((S)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide (67 mg, yield: 76%) as a yellow liquid. 
     MS m/z (ESI): 314.0 [M+H] + . 
     Step 8: Preparation of (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-4,6-Dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide (67 mg, 0.21 mmol), 6-chloro-3-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-amine (78 mg, 0.25 mmol) and DIPEA (0.4 mL) were dissolved in DMF (2.0 mL). The reaction solution was heated to 110° C. and stirred for 10 hours. The reaction solution was dissolved in ethyl acetate (100 mL), washed with water (50 mL) twice, and washed with saturated brine (50 mL) three times. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product, which was purified by column chromatography (EtOAc) to obtain the target product (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide (30 mg, yield: 19%) as a yellow liquid. 
     MS m/z (ESI): 590.0 [M+H] + . 
     Step 9: Preparation of (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-1′-(6-Amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide (30 mg, 0.05 mmol) was dissolved in anhydrous methanol (3.0 mL). Hydrochloric acid/1,4-dioxane (1.0 mL) was added, and the reaction solution was stirred at room temperature for 0.5 hour. The reaction solution was concentrated to obtain a crude product, which was dissolved in water. Saturated sodium bicarbonate solution was added to adjust the pH to 10, and the reaction solution was extracted with dichloromethane (50 mL) twice. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product, which was purified by preparative HPLC to obtain the target product (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine (3.4 mg, yield: 49%) as a yellow solid. 
       1 H NMR (500 MHz, CDCl 3 ) δ 8.77 (s, 1H), 8.48 (s, 1H), 7.45 (d, J=9.7 Hz, 2H), 4.45 (s, 1H), 3.38 (d, J=61.4 Hz, 4H), 2.91 (s, 2H), 2.22 (s, 1H), 1.92 (s, 1H), 1.71 (s, 1H), 1.52 (s, 2H), 1.33-0.92 (m, 8H). 
     MS m/z (ESI): 486 [M+H] + . 
     Example 9 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of (R)—N—((S)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methyl propane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     Tert-butyl (S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidine]-1′-carboxylate (56 mg, 0.13 mmol) was dissolved in anhydrous CH 2 Cl 2  (5 mL). Trifluoroacetic acid (1.0 mL) was added, and the reaction solution was stirred at room temperature for 2 hours. The reaction solution was concentrated to obtain the crude product (R)—N—((S)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methyl propane-2-sulfinamide (40 mg, yield: 100%) as a yellow liquid. 
     MS m/z (ESI): 348.0 [M+H] + . 
     Step 2: Preparation of (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-2-Chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-m ethylpropane-2-sulfinamide (40 mg, 0.1 mmol), 6-chloro-3-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-amine (15 mg, 0.05 mmol) and DIPEA (0.1 mL) were dissolved in DMF (0.8 mL). The reaction solution was heated to 110° C. and stirred for 10 hours. The reaction solution was dissolved in ethyl acetate (100 mL), washed with water (50 mL) twice, and washed with saturated brine (50 mL) three times. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product, which was purified by column chromatography (EtOAc) to obtain the target product (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide (8.2 mg, yield: 26%) as a yellow liquid. 
     MS m/z (ESI): 624.1 [M+H] + . 
     Step 3: Preparation of (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-1′-(6-Amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide (8.2 mg, 0.01 mmol) was dissolved in anhydrous methanol (2.0 mL). Hydrochloric acid/dioxane (0.5 mL) was added, and the reaction solution was stirred at room temperature for 0.5 hour. The reaction solution was concentrated to obtain a crude product, which was dissolved in water. Saturated sodium bicarbonate solution was added to adjust the pH to 10, and the reaction solution was extracted with dichloromethane (50 mL) twice. The organic layers were combined, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product, which was purified by preparative HPLC to obtain the target product (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-2-chloro-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine (1.5 mg, yield: 22%) as a yellow solid. 
       1 H NMR (500 MHz, CDCl 3 ) δ 8.48 (s, 1H), 7.45 (d, J=10.3 Hz, 2H), 4.71 (s, 1H), 3.41 (d, J=74.5 Hz, 4H), 2.92 (s, 2H), 2.16 (d, J=56.0 Hz, 2H), 1.74 (s, 1H), 1.52 (s, 2H), 1.26 (d, J=15.5 Hz, 4H), 1.03 (d, J=39.0 Hz, 4H). 
     MS m/z (ESI): 520.1 [M+H] + . 
     Example 10 
     Preparation of (S)-1′-(6-amino-5-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 2-ethylhexyl 3-((2,3-dichloropyridin-4-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     2,3-Dichloro-4-iodopyridine (2.0 g, 7.31 mmol), 2-ethylhexyl 3-mercaptopropanoate (1.8 g, 8.24 mmol), palladium acetate (82 mg, 0.37 mmol), Xantphos (254 mg, 0.44 mmol) and diisopropylethylamine (1.9 g, 14.70 mmol) were stirred in 15 mL of dioxane under a nitrogen atmosphere at 100° C. for 5 hours. The reaction solution was cooled to room temperature, and then ethyl acetate was added. The reaction solution was filtered, and the filtrate was concentrated to dryness. The resulting residue was purified by column chromatography (petroleum ether/ethyl acetate=3:1) to obtain the target product 2-ethylhexyl 3-((2,3-dichloropyridin-4-yl)thio)propanoate (910 mg, yield: 34%). 
     MS m/z (ESI): 364.1 [M+H] +   
     Step 2: Preparation of 2-ethylhexyl 3-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     2-Ethylhexyl 3-((2,3-dichloropyridin-4-yl)thio)propanoate (200 mg, 0.55 mmol), azetidine (63 mg, 1.10 mmol) and 1,4-dioxane (5 mL) were stirred at 120° C. under microwave for 3 hours. The reaction solution was concentrated to dryness, and then water was added. The solution was extracted with dichloromethane, and the organic phase was concentrated to dryness. The resulting residue was purified by column chromatography (petroleum ether/ethyl acetate=3:1) to obtain the product (130 mg, yield: 62%). 
     MS m/z (ESI): 385.1 [M+H] + . 
     Step 3: Preparation of 2-(azetidin-1-yl)-3-chloropyridine-4-thiol 
     
       
         
         
             
             
         
       
     
     2-Ethylhexyl 3-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)propanoate (130 mg, 0.34 mmol) and potassium tert-butoxide (38 mg, 0.34 mmol) were stirred in anhydrous ethanol (5 mL) at room temperature for 1 hour. The reaction solution was concentrated to dryness, and the resulting residue was used directly in the next step. 
     MS m/z (ESI): 201.0 [M+H] + . 
     Step 4: Preparation of 3-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine 
     
       
         
         
             
             
         
       
     
     2-(Azetidin-1-yl)-3-chloropyridine-4-thiol (obtained in the previous step), 3-bromo-6-chloropyrazin-2-amine (84 mg, 0.40 mmol), Pd 2 (dba) 3  (15 mg, 0.016 mmol), Xantphos (20 mg, 0.035 mmol) and diisopropylethylamine (87 mg, 0.67 mmol) were added to 5 mL of 1,4-dioxane, and the reaction solution was stirred under a nitrogen atmosphere at 100° C. overnight. The reaction solution was cooled to room temperature and concentrated to dryness, and the resulting residue was purified by column chromatography (petroleum ether/ethyl acetate=1:1) to obtain a light yellow solid (71 mg, yield: 64%). 
     MS m/z (ESI): 328.0 [M+H] + . 
     Step 5: Preparation of (R)—N—((S)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     Tert-butyl (S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-1,3-dihydrospiro[indene-2,4′-piperidine]-1′-carboxylate (100 mg, 0.25 mmol) was dissolved in dichloromethane (1 mL). 1 mL of trifluoroacetic acid was added, and the reaction solution was stirred at room temperature for 1 hour. The reaction solution was concentrated to dryness to obtain an oil, which was used directly in the next step. 
     MS m/z (ESI): 307.1 [M+H] + . 
     [α] 20   D =1.773. 
     Step 6: Preparation of (R)—N—((S)-1′-(6-amino-5-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     3-((2-(Azetidin-1-yl)-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine (71 mg, 0.22 mmol), (R)—N—((S)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (obtained in the previous step) and potassium carbonate (299 mg, 2.16 mmol) were stirred in DMF (5 mL) under a nitrogen atmosphere at 100° C. under microwave for 2 hours. Water was added, and the reaction solution was extract with ethyl acetate. The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness to obtain an oil, which was used directly in the next step. 
     MS m/z (ESI): 598.2[M+H] + . 
     Step 7: Preparation of (S)-1′-(6-amino-5-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-1′-(6-Amino-5-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (obtained in the previous step) was dissolved in methanol (5 mL). 4M HCl/1,4-dioxane (5 mL) was added, and the reaction solution was stirred at room temperature for 1 hour. The reaction solution was concentrated to dryness, and the resulting residue was purified by column chromatography to obtain the target product (S)-1′-(6-amino-5-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine (8.7 mg, yield: 8%). 
       1 H NMR (400 MHz, Methanol-d 4 ) δ 7.69 (d, J=5.4 Hz, 1H), 7.58 (s, 1H), 7.42-7.32 (m, 1H), 7.28-7.14 (m, 3H), 5.97 (d, J=5.5 Hz, 1H), 4.35-4.16 (m, 6H), 3.96 (s, 1H), 3.28-3.19 (m, 2H), 3.15 (d, J=15.7 Hz, 1H), 2.81 (d, J=15.7 Hz, 1H), 2.32 (q, J=7.5 Hz, 2H), 1.80 (dtd, J=32.3, 12.6, 4.3 Hz, 2H), 1.58 (d, J=13.4 Hz, 1H), 1.42 (d, J=13.4 Hz, 1H), 1.31 (d, J=16.2 Hz, 1H). 
     MS m/z (ESI): 494.1 [M+H] + . 
     Example 11 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-morpholinopyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 2-ethylhexyl 3-((3-chloro-2-morpholinopyridin-4-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     2-Ethylhexyl 3-((2,3-dichloropyridin-4-yl)thio)propanoate (200 mg, 0.55 mmol), morpholine (96 mg, 1.10 mmol) and 1,4-dioxane (5 mL) were stirred at 120° C. under microwave for 8 hours. The reaction solution was concentrated to dryness, and then water was added. The solution was extracted with dichloromethane, and the organic phase was concentrated to dryness. The resulting residue was purified by column chromatography (petroleum ether/ethyl acetate=3:1) to obtain the product (152 mg, yield: 67%). 
     MS m/z (ESI): 415.2 [M+H] + . 
     Step 2: Preparation of potassium 3-chloro-2-morpholinopyridine-4-thiolate 
     
       
         
         
             
             
         
       
     
     2-Ethylhexyl 3-((3-chloro-2-morpholinopyridin-4-yl)thio)propanoate (100 mg, 0.24 mmol) and potassium tert-butoxide (27 mg, 0.24 mmol) were stirred in anhydrous ethanol (5 mL) at room temperature for 1 hour. The reaction solution was concentrated to dryness to obtain the product, which was used directly in the next step. 
     MS m/z (ESI): 231.0 [M+H] + . 
     Step 3: Preparation of 6-chloro-3-((3-chloro-2-morpholinopyridin-4-yl)thio)pyrazin-2-amine 
     
       
         
         
             
             
         
       
     
     Potassium 3-chloro-2-morpholinopyridine-4-thiolate (obtained in the previous step), 3-bromo-6-chloropyrazin-2-amine (60 mg, 0.29 mmol), Pd 2 (dba) 3  (11 mg, 0.012 mmol), Xantphos (14 mg, 0.024 mmol) and diisopropylethylamine (62 mg, 0.48 mmol) were stirred in 5 mL of dioxane under a nitrogen atmosphere at 100° C. overnight. The reaction solution was cooled to room temperature and concentrated to dryness, and the resulting residue was purified by column chromatography (petroleum ether/ethyl acetate=1:1) to obtain a light yellow solid (60 mg, yield: 70%). 
     MS m/z (ESI): 358.0 [M+H] + . 
     Step 4: Preparation of (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-morpholinopyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     6-Chloro-3-((3-chloro-2-morpholinopyridin-4-yl)thio)pyrazin-2-amine (60 mg, 0.22 mmol), (R)—N—((S)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (76 mg, 0.25 mmol) and potassium carbonate (231 mg, 1.67 mmol) were dissolved in DMF (5 mL), and the reaction solution was stirred under a nitrogen atmosphere at 100° C. under microwave for 2 hours. Water was added, and the reaction solution was extract with ethyl acetate. The organic phase was dried over anhydrous sodium sulfate, and concentrated to dryness to obtain an oil, which was used directly in the next step. 
     MS m/z (ESI): 628.2[M+H] + . 
     Step 5: Preparation of (S)-1′-(6-amino-5-((3-chloro-2-morpholinopyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-morpholinopyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (obtained in the previous step) was dissolved in methanol (5 mL). 4M HCl/1,4-dioxane (5 mL) was added, and the reaction solution was stirred at room temperature for 1 hour. The reaction solution was concentrated to dryness, and the resulting residue was purified by column chromatography to obtain the target product (S)-1′-(6-amino-5-((3-chloro-2-morpholinopyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine (6 mg, yield of two steps: 5%). 
     MS m/z (ESI): 524.1[M+H] + . 
     Example 12 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-(3,3-difluoroazetidin-1-yl)pyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 12 was prepared by referring to the experimental scheme of Example 11. 
     MS m/z (ESI): 530.1 [M+H] + , 532.1[M+2+H] + . 
     Example 13 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-(pyrrolidin-1-yl)pyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 13 was prepared by referring to the experimental scheme of Example 11. 
     MS m/z (ESI): 508.1 [M+H] + , 510.1[M+2+H] + . 
     Example 14 
     Preparation of (S)-1′-(6-amino-5-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 14 was prepared by referring to the experimental scheme of Example 11. 
     MS m/z (ESI): 508.1 [M+H] + , 510.1[M+2+H] + . 
     Example 15 
     Preparation of (S)-1′-(6-amino-5-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1-methyl-4,6-dihydro-1H-spiro[cyclopenta[d]imidazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 15 was prepared by referring to the experimental scheme of Example 11. 
     MS m/z (ESI): 498.1 [M+H] + , 500.1[M+2+H] + . 
     Example 16 
     Preparation of (S)-1′-(6-amino-5-((2-(azetidin-1-yl)-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-methyl-2,6-dihydro-4H-spiro[cyclopenta[c]pyrazole-5,4′-piperidin]-4-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 16 was prepared by referring to the experimental scheme of Example 11. 
     MS m/z (ESI): 498.1 [M+H] + , 500.1[M+2+H] + . 
     Example 17 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-morpholinopyridin-4-yl)thio)-3-methylpyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 17 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 538.1 [M+H] + , 540.1[M+2+H] + . 
     Example 18 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-(pyrrolidin-1-yl)pyridin-4-yl)thio)-3-methylpyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 18 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 522.1 [M+H] + , 524.1[M+2+H] + . 
     Example 19 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-(pyrrolidin-1-yl)pyridin-4-yl)thio)-3-methylpyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 19 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 529.1 [M+H] + , 531.1[M+2+H] + . 
     Example 20 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-(pyrrolidin-1-yl)pyridin-4-yl)thio)-3-methylpyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 20 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 513.1 [M+H] + , 515.1[M+2+H] + . 
     Example 21 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-(pyrrolidin-1-yl)pyridin-4-yl)thio)-3-methylpyrazin-2-yl)-1-methyl-4,6-dihydro-1H-spiro[cyclopenta[d]imidazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 21 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 526.1 [M+H] + , 528.1[M+2+H] + . 
     Example 22 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-(pyrrolidin-1-yl)pyridin-4-yl)thio)-3-methylpyrazin-2-yl)-2-methyl-2,6-dihydro-4H-spiro[cyclopenta[c]pyrazole-5,4′-piperidin]-4-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 22 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 526.1 [M+H] + , 528.1[M+2+H] + . 
     Example 23 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-morpholinopyridin-4-yl)thio)-3-methylpyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 23 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 529.1 [M+H] + , 531.1[M+2+H] + . 
     Example 24 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-morpholinopyridin-4-yl)thio)-3-methylpyrazin-2-yl)-2-methyl-2,6-dihydro-4H-spiro[cyclopenta[c]pyrazole-5,4′-piperidin]-4-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 24 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 542.1 [M+H] + , 544.1[M+2+H] + . 
     Example 25 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)-3-methylpyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 25 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 484.1 [M+H] + , 486.1[M+2+H] + . 
     Example 26 
     Preparation of (S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 26 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 470.1 [M+H] + , 472.1[M+2+H] + . 
     Example 27 
     Preparation of (S)-1′-(6-amino-5-((2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 27 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 436.1 [M+H] + . 
     Example 28 
     Preparation of (S)-1′-(6-amino-5-((2-cyclopropyl-3-methylpyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 28 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 450.1 [M+H] + . 
     Example 29 
     Preparation of (S)-1′-(6-amino-5-((2-cyclopropyl-3-fluoropyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 29 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 454.1 [M+H] + . 
     Example 30 
     Preparation of (S)-1-(4-((3-amino-5-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)pyrazin-2-yl)thio)-3-chloropyridin-2-yl)azetidin-2-one 
     
       
         
         
             
             
         
       
     
     The compound of Example 30 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 508.1 [M+H] + , 510.1[M+2+H] + . 
     Example 31 
     Preparation of (S)-1-(4-((3-amino-5-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)pyrazin-2-yl)thio)-3-chloropyridin-2-yl)pyrrolidin-2-one 
     
       
         
         
             
             
         
       
     
     The compound of Example 31 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 522.1 [M+H] + , 524.1[M+2+H] + . 
     Example 32 
     Preparation of (S)-1-(3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-((2-cyclopropylpyridin-4-yl)thio)-5-methylpyrazin-2-yl)cyclopropan-1-ol 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of (S)-1-(3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-((2-cyclopropylpyridin-4-yl)thio)-5-methylpyrazin-2-yl)cyclopropan-1-ol 
     
       
         
         
             
             
         
       
     
     Ethylmagnesium bromide (0.1 mL, 0.30 mmol, 3M) and tetraisopropyl titanate (43 mg, 0.15 mmol) were added dropwise to a solution of ethyl (S)-3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-(2-cyclopropylpyridin-4-yl)-5-methylpyrazine-2-carboxylate (50 mg, 0.10 mmol) in THE (10 mL) at 0° C. The reaction solution was warmed up to room temperature and stirred for 5 hours. Saturated NH 4 Cl aqueous solution was added to quench the reaction, and the reaction solution was extracted with dichloromethane (3*20 mL). The organic phase was dried over anhydrous sodium sulfate, concentrated and purified by preparative HPLC to obtain (S)-1-(3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-((2-cyclopropylpyridin-4-yl)-5-methylpyrazin-2-yl)cyclopropan-1-ol (5 mg, yield: 10%) as a white solid. 
     MS m/z (ESI): 500.1 [M+H] + . 
     Example 33 
     Preparation of (S)-1-(3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-((3-chloro-2-cyclopropylpyridin-4-yl)thio)-5-methylpyrazin-2-yl)cyclopropan-1-ol 
     
       
         
         
             
             
         
       
     
     The compound of Example 33 was prepared by referring to the experimental scheme of Example 32. 
     MS m/z (ESI): 534.1 [M+H] + , 536.1[M+2+H] + . 
     Example 34 
     Preparation of (S)-1-(3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-((2-amino-3-chloropyridin-4-yl)thio)-5-methylpyrazin-2-yl)cyclopropan-1-ol 
     
       
         
         
             
             
         
       
     
     The compound of Example 34 was prepared by referring to the experimental scheme of Example 32. 
     MS m/z (ESI): 509.1 [M+H] + , 511.1[M+2+H] + . 
     Example 35 
     Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of tert-butyl 4-allyl-4-formylpiperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     Lithium tert-butoxide (13.5 g, 16.9 mmol) was added to a solution of tert-butyl 4-formylpiperidine-1-carboxylate (30 g, 14.1 mmol) in N,N-dimethylformamide (300 mL) at 0° C., and the solution was stirred for 30 minutes after completion of the addition. Allyl bromide (19 g, 16.2 mmol) was added, and the reaction solution was stirred at 0° C. for 2 hours after completion of the addition. The reaction solution was poured into aqueous ammonium chloride solution (1 L), and extracted with ethyl acetate (1 L×2). The ethyl acetate layer was washed with saturated sodium chloride aqueous solution (500 mL), dried over anhydrous sodium sulfate, and purified by column chromatography [eluent: petroleum ether˜ethyl acetate/petroleum ether from 0% to 2%] to obtain the product tert-butyl 4-allyl-4-formylpiperidine-1-carboxylate (15 g, yield: 42%) as a colorless oil. 
     Step 2: Preparation of tert-butyl 4-allyl-4-(1-hydroxyallyl)piperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     Vinyl magnesium chloride (65 mL, 123.5 mmol) was added dropwise to a solution of tert-butyl 4-allyl-4-formylpiperidine-1-carboxylate (25 g, 99 mmol) in tetrahydrofuran (300 mL) at −78° C. After completion of the addition, the reaction solution was slowly warmed up to room temperature and stirred for 30 minutes. The reaction solution was poured into aqueous ammonium chloride solution (1 L), and extracted with ethyl acetate (1 L×2). The ethyl acetate layer was washed with saturated sodium chloride aqueous solution (500 mL), dried over anhydrous sodium sulfate, and purified by column chromatography [eluent: petroleum ether˜ethyl acetate/petroleum ether from 0% to 5%] to obtain the product tert-butyl 4-allyl-4-(1-hydroxyallyl)piperidine-1-carboxylate (25 g, yield: 90%) as a colorless oil. 
     Step 3: Preparation of tert-butyl 4-acryloyl-4-allylpiperidine-1-carboxylate 
     
       
         
         
             
             
         
       
     
     Dess-Martin reagent (41.5 g, 98 mmol) was added to a solution of tert-butyl 4-allyl-4-(1-hydroxyallyl)piperidine-1-carboxylate (25 g, 89 mmol) in dichloromethane (400 mL) at 0° C. After completion of the addition, the reaction solution was stirred at 40° C. for 1 hour. The reaction solution was slowly poured into sodium bicarbonate/sodium sulfite aqueous solution (1/1, 1 L), and extracted with dichloromethane (1 L×2). The dichloromethane layer was washed with saturated sodium chloride aqueous solution (500 mL), dried over anhydrous sodium sulfate, and concentrated to dryness by rotary evaporation. N-heptane (200 mL) was added, and the solution was stirred for 5 minutes. The solution was filtered to remove insoluble substance, and the filtrate was concentrated to obtain the product tert-butyl 4-acryloyl-4-allylpiperidine-1-carboxylate (24.8 g, yield: 100%) as a colorless oil, which was rapidly used in the next step. 
     Step 4: Preparation of tert-butyl 1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate 
     
       
         
         
             
             
         
       
     
     Tert-butyl 4-acryloyl-4-allylpiperidine-1-carboxylate (24.8 g, 89 mmol) and dichloro(2-isopropoxyphenylmethylene)(tricyclohexylphosphine)ruthenium(II) (1.2 g, 1.4 mmol) were stirred in toluene (700 mL) at 90° C. for 2 hours. The reaction solution was cooled, concentrated and purified by column chromatography [eluent: petroleum ether˜ethyl acetate/petroleum ether from 0% to 20%] to obtain the product tert-butyl 1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate (13 g, yield: 58%) as a reddish black solid. 
     MS m/z (ESI): 252.1 [M+H] + . 
     Step 5: Preparation of tert-butyl 2-bromo-1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate 
     
       
         
         
             
             
         
       
     
     Pyridine N-oxide (11 g, 116 mmol) was added to a solution of tert-butyl 1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate (13 g, 51.8 mmol) in acetonitrile (300 mL), followed by the addition of N-bromosuccinimide (28 g, 157 mmol). After completion of the addition, the reaction solution was stirred at 80° C. for 18 hours. The reaction solution was cooled, concentrated and purified by column chromatography [eluent: petroleum ether˜ethyl acetate/petroleum ether from 0% to 20%] to obtain the product tert-butyl 2-bromo-1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate (4.2 g, yield: 25%) as a yellow solid. 
     MS m/z (ESI): 330.1 [M+H] + , 332.1 [M+2+H] + . 
     Step 6: Preparation of tert-butyl 2-cyclopropyl-1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate 
     
       
         
         
             
             
         
       
     
     Tert-butyl 2-bromo-1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate (4.2 g, 12.8 mmol), potassium cyclopropyl trifluoroborate (3.79 g, 25.6 mmol), cesium carbonate (12.5 g, 38.4 mmol) and [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium dichloromethane complex (731 mg, 0.9 mmol) were stirred in dioxane (100 mL) and water (10 mL) at 100° C. for 18 hours. Water (150 mL) was added to quench the reaction, and the reaction solution was extracted with ethyl acetate (150 mL×2). The ethyl acetate layer was washed with saturated sodium chloride aqueous solution (50 mL), dried over anhydrous sodium sulfate, and purified by column chromatography [eluent: petroleum ether˜ethyl acetate/petroleum ether from 0% to 12%] to obtain the product tert-butyl 2-cyclopropyl-1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate (1 g, yield: 27%) as a yellow oil. 
     MS m/z (ESI): 292.2 [M+H] +   
     Step 7: Preparation of tert-butyl (R,Z)-1-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-2-cyclopropyl-8-azaspiro[4.5]dec-2-ene-8-carboxylate 
     
       
         
         
             
             
         
       
     
     Tert-butyl 2-cyclopropyl-1-oxo-8-azaspiro[4.5]dec-2-ene-8-carboxylate (1 g, 3.4 mmol) and (R)-(+)-tert-butylsulfinamide (2 g, 17.2 mmol) were stirred in tetraethyl titanate (30 mL) at 100° C. for 18 hours. The reaction solution was cooled, and then ethyl acetate (100 mL) was added. The reaction solution was poured into water (150 mL) to precipitate a large amount of white solid. The reaction solution was filtered through diatomaceous earth, and the filtrate was separated into two phases. The organic phase was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography [eluent: petroleum ether˜ethyl acetate/petroleum ether from 0% to 50%] obtain the product tert-butyl (R,Z)-1-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-2-cyclopropyl-8-azaspiro[4.5]dec-2-ene-8-carboxylate (190 mg, yield: 14%) as a yellow oil. 
     MS m/z (ESI): 395.2 [M+H] + . 
     Step 8: Preparation of tert-butyl (S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-2-cyclopropyl-8-azaspiro[4.5]dec-2-ene-8-carboxylate 
     
       
         
         
             
             
         
       
     
     Diisobutyl aluminum hydride (0.9 mL, 1 mmol) was added dropwise to a solution of tert-butyl (R,Z)-1-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-2-cyclopropyl-8-azaspiro[4.5]dec-2-ene-8-carboxylate (210 mg, 0.53 mmol) in tetrahydrofuran (10 mL) at −78° C. After completion of the addition, the reaction solution was stirred for 15 minutes. Sodium sulfate decahydrate was added at −78° C. to quench the reaction, and the reaction solution was stirred for 10 minutes. The reaction solution was filtered, and the filtrate was concentrated and purified by column chromatography [eluent: petroleum ether˜ethyl acetate/petroleum ether from 0% to 5%] to obtain the product tert-butyl (S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-2-cyclopropyl-8-azaspiro[4.5]dec-2-ene-8-carboxylate (105 mg, yield: 50%) as a colorless oil. 
     MS m/z (ESI): 397.2 [M+H] + . 
     Step 9: Preparation of (S)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     Hydrochloric acid in dioxane (15 mL, 60 mmol) was added to a solution of tert-butyl (S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-2-cyclopropyl-8-azaspiro[4.5]dec-2-ene-8-carboxylate (105 mg, 0.27 mmol) in methanol (3 mL). After completion of the addition, the reaction solution was stirred at room temperature for 1 hour. The reaction solution was concentrated to dryness by rotary evaporation to obtain the product (S)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine (105 mg, yield: 100%) as a white solid. 
     MS m/z (ESI): 193.2 [M+H] + . 
     Step 10: Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     3-((2-Amino-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine (76 mg, 0.26 mmol), (S)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine (80 mg, 0.2 mmol) and potassium carbonate (167 mg, 1.2 mmol) were stirred in N,N-dimethylformamide (8 mL) at 100° C. for 7 hours. Water (60 mL) was added, and the reaction solution was extracted with ethyl acetate (50 mL×2). The ethyl acetate layer was washed with saturated sodium chloride aqueous solution (20 mL), dried over anhydrous sodium sulfate, and purified by thin layer chromatography [eluent: dichloromethane/methanol=7/1] to obtain the product (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine (37 mg, yield: 41%) as a yellow oil. 
       1 H NMR (400 MHz, MeOD) δ 7.67-7.51 (m, 2H), 5.92 (d, J=8 Hz, 1H), 5.27 (s, 1H), 4.26-4.04 (m, 2H), 3.33 (s, 1H), 3.27-3.08 (m, 2H), 2.44-2.18 (m, 2H), 1.81-1.69 (m, 1H), 1.68-1.47 (m, 2H), 1.42-1.24 (m, 2H), 0.81-0.60 (m, 2H), 0.57-0.28 (m, 2H). 
     MS m/z (ESI): 444.1 [M+H] + , 446.1 [M+2+H] + . 
     Example 36 
     Preparation of (S)-8-(5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 3-chloro-4-((5-chloropyrazin-2-yl)thio)pyridin-2-amine 
     
       
         
         
             
             
         
       
     
     Potassium 2-amino-3-chloropyridine-4-thiolate (1 g, 2.64 mmol), 2-bromo-5-chloropyrazine (597 mg, 3.11 mmol), tris(dibenzylideneacetone)dipalladium (284 mg, 0.311 mmol), chloro[(4,5-bis(diphenylphosphino)-9,9-dimethylxanthene)-2-(2′-amino-1,1′-biphenyl)]palladium(II) (360 mg, 0.622 mmol) and N,N-diisopropylethylamine (1.2 g, 9.33 mmol) were stirred in N,N-dimethylformamide (10 mL) at 90° C. under microwave for 1 hour. The reaction solution was concentrated to dryness by rotary evaporation, and the resulting residue was purified by column chromatography [eluent: petroleum ether˜ethyl acetate/petroleum ether from 0% to 30%] to obtain the product 3-chloro-4-((5-chloropyrazin-2-yl)thio)pyridin-2-amine (250 mg, yield: 35%) as a white solid. 
     MS m/z (ESI): 273.0 [M+H] + , 275.0 [M+2+H] + . 
     Step 2: Preparation of (S)-8-(5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     3-Chloro-4-((5-chloropyrazin-2-yl)thio)pyridin-2-amine (100 mg, 0.368 mmol), (S)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine hydrochloride (105 mg, 0.265 mmol) and potassium carbonate (254 mg, 1.84 mmol) were stirred in N,N-dimethylformamide (8 mL) at 100° C. for 6 hours. Water (40 mL) was added, and the reaction solution was extracted with ethyl acetate (40 mL*2). The organic phase was concentrated, and purified by thin layer chromatography (dichloromethane/methanol=10/1) to obtain the product (S)-8-(5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine (40 mg, yield: 35%) as a yellow solid. 
       1 H NMR (400 MHz, MeOD) δ 8.29 (d, J=16 Hz, 2H), 7.60 (d, J=4 Hz, 1H), 5.93 (d, J=4 Hz, 1H), 5.31 (s, 1H), 4.34-4.14 (m, 2H), 3.43-3.35 (m, 2H), 3.28-3.21 (m, 1H), 2.45-2.21 (m, 2H), 1.86-1.49 (m, 3H), 1.44-1.22 (m, 2H), 0.82-0.61 (m, 2H), 0.58-0.30 (m, 2H). 
     MS m/z (ESI): 429.1 [M+H] + , 431.1 [M+2+H] + . 
     Example 37 
     Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-2-methyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 37 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 432.1 [M+H] + , 434.1[M+2+H] + . 
     Example 38 
     Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-2-ethyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 38 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 446.1 [M+H] + , 448.1[M+2+H] + . 
     Example 39 
     Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-2-isopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 39 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 460.1 [M+H] + , 462.1[M+2+H] + . 
     Example 40 
     Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 40 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 458.1 [M+H] + , 460.1[M+2+H] + . 
     Example 41 
     Preparation of (S)-1-(6-((2-amino-3-chloropyridin-4-yl)thio)-3-(6-amino-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-1′-yl)-5-methylpyrazin-2-yl)cyclopropan-1-ol 
     
       
         
         
             
             
         
       
     
     The compound of Example 41 was prepared by referring to the experimental scheme of Example 32. 
     MS m/z (ESI): 500.1 [M+H] + , 502.1[M+2+H] + . 
     Example 42 
     Preparation of (R)-1-(6-((2-amino-3-chloropyridin-4-yl)thio)-3-(1-amino-8-azaspiro[4.5]dec-2-en-8-yl)-5-methylpyrazin-2-yl)cyclopropan-1-ol 
     
       
         
         
             
             
         
       
     
     The compound of Example 42 was prepared by referring to the experimental scheme of Example 32. 
     MS m/z (ESI): 459.1 [M+H] + , 461.1[M+2+H] + . 
     Example 43 
     Preparation of (S)-1-(3-(1-amino-2-methyl-8-azaspiro[4.5]dec-2-en-8-yl)-6-((2-amino-3-chloropyridin-4-yl)thio)-5-methylpyrazin-2-yl)cyclopropan-1-ol 
     
       
         
         
             
             
         
       
     
     The compound of Example 43 was prepared by referring to the experimental scheme of Example 32. 
     MS m/z (ESI): 473.1 [M+H] + , 475.1[M+2+H] + . 
     Example 44 
     Preparation of (S)-1-(3-(1-amino-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-8-yl)-6-((2-amino-3-chloropyridin-4-yl)thio)-5-methylpyrazin-2-yl)cyclopropan-1-ol 
     
       
         
         
             
             
         
       
     
     The compound of Example 44 was prepared by referring to the experimental scheme of Example 32. 
     MS m/z (ESI): 499.1 [M+H] + , 501.1[M+2+H] + . 
     Example 45 
     Preparation of (S)-(3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-((2-amino-3-chloropyridin-4-yl)thio)-5-methylpyrazin-2-yl)methanol 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 2-ethylhexyl 3-((2-amino-3-chloropyridin-4-yl)thio)propanoate 
     
       
         
         
             
             
         
       
     
     3-Chloro-4-iodopyridin-2-amine (3 g, 11.8 mmol), 2-ethylhexyl 3-mercaptopropanoate (2.9 g, 14.2 mmol), palladium acetate (132 mg, 0.59 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (683 mg, 1.18 mmol) and N,N-diisopropylethylamine (561 mg, 4.3 mmol) were stirred in dioxane (80 mL) at 100° C. for 18 hours. The reaction solution was filtered. The filtrate was concentrated, and purified by column chromatography [eluent: petroleum ether˜petroleum ether/ethyl acetate (60:40)] to obtain 2-ethylhexyl 3-((2-amino-3-chloropyridin-4-yl)thio)propanoate (3.8 g, yield: 94%) as a light yellow solid. 
     MS m/z (ESI): 345.1 [M+H] + , 347.0 [M+2+H] + . 
     Step 2: Preparation of potassium 2-amino-3-chloropyridine-4-thiolate 
     
       
         
         
             
             
         
       
     
     Potassium tert-butoxide (174 mg, 1.53 mmol) was added to a solution of 2-ethylhexyl 3-((2-amino-3-chloropyridin-4-yl)thio)propanoate (500 mg, 1.5 mmol) in ethanol (30 mL). After completion of the addition, the reaction solution was stirred at 40° C. for 2 hours. The reaction solution was concentrated to obtain the crude product potassium 2-amino-3-chloropyridine-4-thiolate (500 mg) as a yellow solid, which was used directly in the next step. 
     MS m/z (ESI): 161.0 [M+H] + , 163.0 [M+2+H] + . 
     Step 3: Preparation of ethyl 6-bromo-3-((S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate 
     
       
         
         
             
             
         
       
     
     Ethyl 6-bromo-3-chloro-5-methylpyrazine-2-carboxylate (840 mg, 3 mmol), (S)—N-(1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)pivalamide trifluoroacetate (1.5 g, 3.6 mmol) and potassium carbonate (1.7 g, 12 mmol) were stirred in acetonitrile (40 mL) at 55° C. for 18 hours. The reaction solution was filtered. The filtrate was concentrated, and purified by column chromatography [eluent: petroleum ether˜petroleum ether/ethyl acetate (60:40)] to obtain ethyl 6-bromo-3-((S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (1.5 g, yield: 91%) as a yellow solid. 
     MS m/z (ESI): 549.2 [M+H] + , 551.2 [M+2+H] + . 
     Step 4: Preparation of ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate 
     
       
         
         
             
             
         
       
     
     Ethyl 6-bromo-3-((S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (798 mg, 1.45 mmol), potassium 2-amino-3-chloropyridine-4-thiolate (500 mg, 1.45 mmol, crude), tris(dibenzylideneacetone)dipalladium (66 mg, 0.073 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (84 mg, 0.145 mmol) and N,N-diisopropylethylamine (561 mg, 4.35 mmol) were stirred in N,N-dimethylformamide (15 mL) at 100° C. under microwave for 1.5 hours. The reaction solution was filtered. The filtrate was concentrated, and purified by column chromatography [eluent: dichloromethane˜dichloromethane/methanol (98.5:1.5)] to obtain ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (500 mg, yield: 55%) as a yellow solid. 
     MS m/z (ESI): 629.1 [M+H] + , 631.1 [M+2+H] + . 
     Step 5: Preparation of (R)—N—((S)-1′-(5-((2-amino-3-chloropyridin-4-yl)thio)-3-(hydroxymethyl)-6-methylpyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     Lithium aluminum hydride (0.7 mL, 2.5 M) was added dropwise to a solution of ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-1-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (500 mg, 0.795 mmol) in tetrahydrofuran (30 mL) at 0° C. The reaction solution was stirred at 0° C. for 30 minutes. Sodium sulfate decahydrate was added to quench the reaction, and the reaction solution was stirred for 10 minutes. The reaction solution was filtered through diatomaceous earth, and the filter cake was washed with tetrahydrofuran (50 mL) twice. The filtrate was concentrated, and purified by column chromatography [eluent: dichloromethane dichloromethane/methanol (95:5)] to obtain (R)—N—((S)-1′-(5-((2-amino-3-chloropyridin-4-yl)thio)-3-(hydroxymethyl)-6-methylpyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (220 mg, yield: 47%) as a yellow solid. 
     MS m/z (ESI): 587.1 [M+H] + , 589.1 [M+2+H] + . 
     Step 6: Preparation of (S)-(3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-((2-amino-3-chloropyridin-4-yl)thio)-5-methylpyrazin-2-yl)methanol 
     
       
         
         
             
             
         
       
     
     Hydrochloric acid in dioxane (20 mL, 4.0 M) was added to a solution of (R)—N—((S)-1′-(5-((2-amino-3-chloropyridin-4-yl)thio)-3-(hydroxymethyl)-6-methylpyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (220 mg, 0.375 mmol) in methanol (3 mL), and the reaction solution was stirred at room temperature for 30 minutes. The reaction solution was concentrated, and dissolved in methanol (10 mL). The reaction solution was adjusted to alkaline with ammonia, and concentrated to dryness by rotary evaporation. The resulting residue was purified by column chromatography [eluent: dichloromethane˜dichloromethane/methanol (1% NH3-H2O) (95:5)] to obtain (S)-(3-(1-amino-1,3-dihydrospiro[indene-2,4′-piperidin]-1′-yl)-6-((2-amino-3-chloropyridin-4-yl)thio)-5-methylpyrazin-2-yl)methanol (115 mg, yield: 63%) as a white solid. 
       1 H NMR (400 MHz, MeOD) δ 7.59 (d, J=4 Hz, 1H), 7.41-7.34 (m, 1H), 7.27-7.16 (m, 3H), 5.89 (d, J=4 Hz, 1H), 4.64 (s, 2H), 4.00 (s, 1H), 3.88 (d, J=12 Hz, 2H), 3.28-3.20 (m, 2H), 3.14 (d, J=16 Hz, 1H), 2.82 (d, J=16 Hz, 1H), 2.48 (s, 3H), 2.01-1.85 (m, 2H), 1.67-1.58 (m, 1H), 1.52-1.43 (m, 1H). 
     MS m/z (ESI): 483.2 [M+H] + , 485.2 [M+2+H] + . 
     Example 46 
     Preparation of (S)-(6-((2-amino-3-chloropyridin-4-yl)thio)-3-(6-amino-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazin-2-yl)methanol 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of ethyl 6-bromo-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-4,6-Dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide (470 mg, 1.5 mmol, 1.0 eq), ethyl 6-bromo-3-chloro-5-methylpyrazine-2-carboxylate (398 mg, 1.43 mmol, 0.95 eq) and potassium carbonate (1.04 g, 7.5 mmol, 5.0 eq) were dissolved in DMF (4.0 mL). The reaction solution was heated to 60° C. under a nitrogen atmosphere and stirred for 3 hours. After completion of the reaction, the reaction solution was concentrated to remove DMF. The resulting crude product was purified by column chromatography (100% EtOAc in Petro ether) to obtain the product ethyl 6-bromo-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (600 mg, yield: 72%) as a yellow solid. 
     MS m/z (ESI): 556.1[M+H] + , 558.1[M+H+2] + . 
     Step 2: Preparation of ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate 
     
       
         
         
             
             
         
       
     
     In a microwave reaction tube, ethyl 6-bromo-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (600 mg, 1.08 mmol, 1.0 eq) and potassium 2-amino-3-chloropyridine-4-thiolate (536 mg, 2.70 mmol, 2.5 eq) were dissolved in 10 mL of DMF, followed by the addition of tris(dibenzylideneacetone)dipalladium (50 mg, 0.054 mmol, 0.05 eq), Xantphos (62 mg, 0.11 mmol, 0.1 eq) and DIPEA (419 mg, 3.24 mmol, 3.0 eq). The reaction solution was bubbled with nitrogen for 3 minutes, heated under microwave to 105° C. and reacted for 75 minutes. The reaction solution was cooled to room temperature, diluted with 20 mL of ethyl acetate, and filtered through diatomaceous earth. The filtrate was concentrated, and the resulting crude product was purified by column chromatography (10% MeOH in CH 2 Cl 2 ) to obtain the product ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (360 mg, yield: 52%) as a yellow solid. 
     MS m/z (ESI): 636.0 [M+H] + , 638.0 [M+H+2] + . 
     Step 3: Preparation of ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate 
     
       
         
         
             
             
         
       
     
     Ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (360 mg, 0.57 mmol, 1.0 eq) was dissolved in 40 mL of anhydrous THF, and the solution was purged with nitrogen. The solution was cooled to 0° C., and then a solution of LiAlH 4  in tetrahydrofuran (0.6 mL, 1.14 mmol, 2.0 eq., D=2M) was added dropwise. After completion of the addition, the reaction solution was slowly warmed up to room temperature and stirred for 3 hours. After completion of the reaction, the reaction solution was diluted with ethyl acetate (100 mL), and cooled to 0° C. 2 mL of water was added dropwise, and 4 mL of 15% sodium hydroxide aqueous solution was added. 6 mL of water was added, and the reaction solution was warmed up to room temperature and stirred for 30 minutes. An appropriate amount of anhydrous sodium sulfate was added, and the reaction solution was stirred for 15 minutes. The reaction solution was filtered to remove the solid, and the filtrate was concentrated. The resulting crude product was purified by column chromatography (10% MeOH in CH 2 Cl 2 ) to obtain the product ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (150 mg, yield: 44%) as a yellow solid. 
     MS m/z (ESI): 594.1[M+H] + , 596.1[M+H+2] + . 
     Step 4: Preparation of (S)-(6-((2-amino-3-chloropyridin-4-yl)thio)-3-(6-amino-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazin-2-yl)methanol 
     
       
         
         
             
             
         
       
     
     Ethyl 6-((2-amino-3-chloropyridin-4-yl)thio)-3-((S)-6-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazine-2-carboxylate (150 mg, 0.25 mmol, 1.0 eq) was dissolved in MeOH (5 mL). A solution of hydrochloric acid in dioxane (1.0 mL, 4M) was added, and the reaction solution was reacted at room temperature for 1 hour. The reaction solution was concentrated to dryness and dissolved in 5 mL of methanol, and the pH was adjusted to alkaline with 7M NH 3  in methanol. The reaction solution was concentrated, and purified by normal-phase column chromatography (10% MeOH in CH 2 Cl 2 ) and reversed-phase column chromatography (30% MeCN in H 2 O (0.1% NH 3 .H 2 O)) successively to obtain the target compound (S)-(6-((2-amino-3-chloropyridin-4-yl)thio)-3-(6-amino-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-1′-yl)-5-methylpyrazin-2-yl)methanol (52 mg, yield: 42%) as a grey solid. 
       1 H NMR (400 MHz, DMSO) δ 8.93 (s, 1H), 7.62 (s, 1H), 6.31 (s, 1H), 5.72 (s, 1H), 5.41 (s, 1H), 4.45 (s, 2H), 3.98 (s, 1H), 3.84 (s, 2H), 2.76 (dd, J=47.2, 16.9 Hz, 4H), 2.37 (s, 3H), 1.60 (dd, J=165.2, 116.4 Hz, 7H). 
     MS m/z (ESI): 490.1 [M+H] + , 492.1 [M+H+2] + . 
     Example 47 
     Preparation of (S)-(6-((2-amino-3-chloropyridin-4-yl)thio)-3-(6-amino-4,6-dihydrospiro[cyclopenta[d]oxazole-5,4′-piperidin]-1′-yl)-5-methylpyrazin-2-yl)methanol 
     
       
         
         
             
             
         
       
     
     The compound of Example 47 was prepared by referring to the experimental scheme of Example 46. 
     MS m/z (ESI): 474.1 [M+H] + , 476.1[M+2+H] + . 
     Example 48 
     Preparation of (R)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-methylene-8-azaspiro[4.5]decan-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 48 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 418.1 [M+H] + , 420.1[M+2+H] + . 
     Example 49 
     Preparation of (S,E)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-ethylidene-8-azaspiro[4.5]decan-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 49 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 432.1 [M+H] + , 434.1[M+2+H] + . 
     Example 50 
     Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-(propan-2-ylidene)-8-azaspiro[4.5]decan-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 50 was prepared by referring to the experimental scheme of Example 1. 
     MS m/z (ESI): 446.1 [M+H] + , 448.1[M+2+H] + . 
     Example 51 
     Preparation of (S,E)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-2-ethylidene-8-azaspiro[4.5]decan-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 51 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 446.1 [M+H] + , 448.1[M+2+H] + . 
     Example 52 
     Preparation of (R)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-methylpyrazin-2-yl)-2-methylene-8-azaspiro[4.5]decan-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 52 was prepared by referring to the experimental scheme of Example 6. 
     MS m/z (ESI): 432.1 [M+H] + , 434.1[M+2+H] + . 
     Example 53 
     Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of (R)—N—((S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     3-((2-Amino-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine (200 mg, 0.694 mmol), (R)—N—((S)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (350 mg, 0.833 mmol, [α] 20   D =1.773) and potassium carbonate (479 mg, 3.47 mmol) were stirred in N,N-dimethylformamide (7 mL) at 100° C. for 8 hours. After cooling, 40 mL of water was added, and the reaction solution was extracted with ethyl acetate (40 mL×2). The ethyl acetate layer was washed with saturated sodium chloride aqueous solution (20 mL), dried over anhydrous sodium sulfate, and purified by column chromatography [eluent: dichloromethane˜dichloromethane/methanol from 0% to 3%] to obtain the product (R)—N—((S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (200 mg, yield: 52%) as an orange solid. 
     MS m/z (ESI): 558.2 [M+H] + . 
     Step 2: Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     Hydrochloric acid in dioxane (20 mL, 80 mmol) was added to a solution of (R)—N—((S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (188 mg, 0.337 mmol) in methanol (5 mL), and the reaction solution was stirred at room temperature for 1 hour. The reaction solution was concentrated to dryness, and dissolved in 5 mL of methanol. The pH was adjusted to alkaline with ammonia. The reaction solution was concentrated and purified by column chromatography [eluent: dichloromethane˜dichloromethane/methanol from 0% to 7.5%] to obtain the product (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine (130 mg, yield: 85%) as a white solid. 
     MS m/z (ESI): 454.2 [M+H] + . 
     Step 3: Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     N-Fluorobisbenzenesulfonamide (69 mg, 0.22 mmol) was added to a solution of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine (100 mg, 0.22 mmol) in acetonitrile (10 mL) at −10° C. After completion of the addition, the reaction solution was slowly warmed up to room temperature and stirred for 7 hours. The reaction solution was concentrated to dryness by rotary evaporation, and the resulting residue was purified by thin layer chromatography (dichloromethane/methanol=10/1) to obtain the product (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine (28 mg, yield: 27%) as a khaki solid. 
       1 H NMR (400 MHz, MeOD) δ 7.62 (d, J=4 Hz, 1H), 7.42-7.33 (m, 1H), 7.26-7.15 (m, 3H), 6.00 (d, J=4 Hz, 1H), 4.67-4.54 (m, 2H), 4.30 (d, J=16 Hz, 2H), 3.97 (s, 1H), 3.16 (d, J=16 Hz, 1H), 2.82 (d, J=16 Hz, 1H), 1.95-1.78 (m, 2H), 1.65-1.53 (m, 1H), 1.48-1.38 (m, 1H). 
     MS m/z (ESI): 472.1 [M+H] + , 474.1 [M+2+H] + . 
     [α] 20   D =−36.893. 
     Example 54 
     (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of 3-bromo-2-(chloromethyl)pyridine 
     
       
         
         
             
             
         
       
     
     (3-Bromopyridin-2-yl)methanol (7.5 g, 40 mmol) was dissolved in dichloromethane (20 mL) and mixed well. SOC 2  (9.5 g, 80 mmol) was added dropwise in an ice water bath, and the reaction solution was stirred for 1 hour. The reaction solution was concentrated under reduced pressure, and the resulting crude product was purified by rapid silica gel column chromatography to obtain the target compound 3-bromo-2-(chloromethyl)pyridine (5 g, yield: 61%) as a colorless liquid. 
     MS m z (ESI): 205.9 [M+H] + . 
     Step 2: Preparation of 1-(tert-butyl) 4-methyl 4-((3-bromopyridin-2-yl)methyl)piperidine-1,4-dicarboxylate 
     
       
         
         
             
             
         
       
     
     1-(Tert-butyl) 4-methyl piperidine-1,4-dicarboxylate (7.7 g, 31.7 mmol) and LDA (18.3 mL, 36.6 mmol) were added to THE (20 mL), and the reaction solution was reacted at −60° C. for 1 hour. 3-Bromo-2-(chloromethyl)pyridine (5 g, 24.4 mmol) was added, and the reaction solution was reacted at −60° C. for 2 hours. The reaction solution was concentrated under reduced pressure, and the resulting crude product was purified by rapid silica gel column chromatography to obtain the target compound 1-(tert-butyl) 4-methyl 4-((3-bromopyridin-2-yl)methyl)piperidine-1,4-dicarboxylate (9 g, yield: 89%) as a light yellow solid. 
     MS m z (ESI): 413.0 [M+H] + . 
     Step 3: Preparation of tert-butyl 5-oxo-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     THF (100 mL) was added to 1-(tert-butyl) 4-methyl 4-((3-bromopyridin-2-yl)methyl)piperidine-1,4-dicarboxylate (9 g, 21.84 mmol), and the solution was cooled to −78° C. N-butyl lithium solution (1.6 N, 17 mL) was slowly added dropwise, and the reaction solution was reacted at −78° C. for 1 hour. Ammonium chloride aqueous solution was added to quench the reaction, and the reaction solution was extracted with ethyl acetate. The organic phase was dried over anhydrous sodium sulfate and filtered, and the filtrate was concentrated under reduced pressure. The resulting crude product was purified by rapid silica gel column chromatography to obtain the target compound tert-butyl 5-oxo-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate (3 g, yield: 45%) as a light yellow solid. 
     MS m z (ESI): 303.1 [M+H] + . 
     Step 4: Preparation of tert-butyl (R,Z)-5-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     Tert-butyl 5-oxo-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate (3 g, 9.93 mmol) and (R)-2-methylpropane-2-sulfinamide (3.6 g, 29.8 mmol) were added to ethyl titanate (50 mL). The reaction solution was stirred well, and reacted at 110° C. for 13 hours. The reaction solution was cooled to room temperature, and water (100 mL) was slowly added dropwise to precipitate a solid. Ethyl acetate (200 mL) was added, and the reaction solution was filtered. The organic phase was concentrated under reduced pressure to obtain the target compound tert-butyl (R,Z)-5-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate (3 g, yield: 75%), which was used directly in the next step. 
     MS m z (ESI): 406.1 [M+H] + . 
     Step 5: Preparation of tert-butyl (S)-5-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate 
     
       
         
         
             
             
         
       
     
     Tert-butyl (R,Z)-5-((tert-butylsulfinyl&lt;sulfenyl&gt;)imino)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate (3 g, 7.41 mmol) was added to THE (40 mL). The solution was cooled to 0° C., and BH 3  (7.5 mL, 2N in THF) was slowly added dropwise. After completion of the addition, the reaction solution was kept at 0° C. and reacted for 2 hours. Methanol (10 mL) was added dropwise, and the reaction solution was concentrated under reduced pressure. The resulting crude product was purified by rapid silica gel column chromatography to obtain the target compound tert-butyl (S)-5-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate (2.5 g, yield: 83%) as a light yellow solid. 
     MS m z (ESI): 408.1 [M+H] + . 
     Step 6: Preparation of (S)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine 
     
       
         
         
             
             
         
       
     
     Tert-butyl (S)-5-(((R)-tert-butylsulfinyl&lt;sulfenyl&gt;)amino)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidine]-1′-carboxylate (2.5 g, 6.14 mmol) and hydrochloric acid in dioxane (10 mL, 4N) were added to CH 2 Cl 2  (40 mL). The reaction solution was stirred at room temperature for 3 hours, and then concentrated under reduced pressure to obtain the target compound (S)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine (1.5 g), which was used directly in the next step. 
     MS m/z (ESI): 204.1 [M+H] + . 
     Step 7: Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine 
     
       
         
         
             
             
         
       
     
     (S)-5,7-Dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine (1.5 g, 7.39 mmol), 3-((2-amino-3-chloropyridin-4-yl)thio)-6-bromopyrazine-2-amine (3.7 g, 11.09 mmol) and cesium carbonate (7.2 g, 22.17 mmol) were added to DMF (50 mL). The reaction solution was reacted at 90° C. for 13 hours. The reaction solution was concentrated under reduced pressure, and the resulting crude product was purified by rapid silica gel column chromatography to obtain the target compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine (1 g, yield: 29%) as a light yellow solid. 
     MS m z (ESI): 455.1 [M+H] + , 457.1 [M+H+2] + . 
     Step 8: Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine 
     
       
         
         
             
             
         
       
     
     (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine (600 mg, 1.32 mmol) and NFSI (830 mg, 2.64 mmol) were added to THE (10 mL). The reaction solution was reacted at room temperature for 3 hours. The reaction solution was concentrated under reduced pressure, and the resulting crude product was purified by rapid silica gel column chromatography to obtain the target compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-5,7-dihydrospiro[cyclopenta[b]pyridine-6,4′-piperidin]-5-amine (90 mg, yield: 14%) as a white solid. 
     MS m z (ESI): 473.0 [M+H] + , 475.1 [M+H+2] + . 
       1 H NMR (400 MHz, MeOH-d 4 ) δ 8.33 (d, J=4.8 Hz, 1H), 7.82 (d, J=7.2 Hz, 1H), 7.62 (d, J=5.6 Hz, 1H), 7.28-7.25 (m, 1H), 6.01 (d, J=5.6 Hz, 1H), 4.35-4.31 (m, 2H), 4.03 (s, 1H), 3.36-3.31 (m, 2H), 3.25-3.21 (m, 1H), 2.92 (d, J=16.8 Hz, 1H), 1.98-1.84 (m, 2H), 1.64-1.60 (m, 1H), 1.42-1.39 (m, 1H). 
     Example 55 
     Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-4,6-Dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-yl)-2-methylpropane-2-sulfinamide (260 mg, 0.83 mmol, 1.0 eq), 3-((2-amino-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine (227 mg, 0.79 mmol, 0.95 eq) and potassium carbonate (574 mg, 4.15 mmol, 5.0 eq) were dissolved in DMF (2 mL). The reaction solution was heated to 105° C. under a nitrogen atmosphere and stirred for 10 hours. After completion of the reaction, the reaction solution was concentrated to remove DMF. The resulting crude product was purified by column chromatography (8% MeOH in CH 2 Cl 2 ) to obtain the product (R)—N—((S)-1′-(6-amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (340 mg, yield: 76%) as a yellow solid. 
     MS m/z (ESI): 565.1[M+H] + , 567.1[M+H+2] + . 
     Step 2: Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     (R)—N—((S)-1′-(6-Amino-5-((3-chloro-2-cyclopropylpyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-yl)-2-methylpropane-2-sulfinamide (340 mg, 0.60 mmol, 1.0 eq) was dissolved in MeOH (5 mL). A solution of HCl in dioxane (1.0 mL, 4M) was added, and the reaction solution was reacted at room temperature for 1 hour. The reaction solution was concentrated to dryness and dissolved in 5 mL of methanol, and the pH was adjusted to alkaline with 7M NH 3  in methanol. The reaction solution was concentrated, and purified by normal-phase column chromatography (10% MeOH in CH 2 Cl 2 ) and reversed-phase column chromatography (40% MeCN in H 2 O (0.1% NH 3 .H 2 O)) successively to obtain the target compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine (220 mg, yield: 80%) as a grey solid. 
     MS m/z (ESI): 461.1 [M+H] + , 463.1 [M+H+2] + . 
     Step 3: Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine 
     
       
         
         
             
             
         
       
     
     (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine (220 mg, 0.48 mmol, 1.0 eq) was dissolved in anhydrous DMF (2 mL) and anhydrous MeCN (10 mL). The reaction solution was purged with nitrogen three times, and the reagent N-fluoro-N-(benzenesulfonyl)benzenesulfonamide (159 mg, 0.5 mmol, 1.05 eq.) was added in batches under a nitrogen atmosphere. After completion of the addition, the reaction solution was stirred at room temperature for 3 hours. The reaction solution was added dropwise to MeOH (50 mL) under stirring, and 1 mL of water was added. The reaction solution was concentrated to obtain a crude product, which was purified by preparative HPLC to obtain the product (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-4,6-dihydrospiro[cyclopenta[d]thiazole-5,4′-piperidin]-6-amine (40 mg, yield: 17%) as a grey solid. 
       1 H NMR (400 MHz, DMSO) δ 8.96 (s, 1H), 7.67 (d, J=5.3 Hz, 1H), 6.28 (s, 2H), 6.17 (s, 2H), 5.86 (d, J=5.4 Hz, 1H), 4.10 (s, 2H), 3.98 (s, 1H), 2.81 (dd, J=52.7, 15.2 Hz, 4H), 1.88 (s, 2H), 1.75-1.52 (m, 4H). 
       19 F NMR (376 MHz, DMSO) δ −88.17 (s). 
     MS m/z (ESI): 479.0 [M+H] + , 481.0 [M+H+2] + . 
     Example 56 
     Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     Step 1: Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     N-fluorobisbenzenesulfonamide (40 mg, 0.128 mmol) was added to a solution of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine (35 mg, 0.085 mmol) in tetrahydrofuran (18 mL) at 25° C. After completion of the addition, the reaction solution was stirred for 1 hour. TLC showed that the reaction was not complete, additional N-fluorobisbenzenesulfonamide (27 mg, 0.085 mmol) was added, and the reaction solution was stirred for another 1 hour. TLC showed that the reaction was not complete, additional N-fluorobisbenzenesulfonamide (27 mg, 0.085 mmol) was added, and the reaction solution was stirred for another 1 hour. TLC showed the presence of impurities. The reaction solution was quenched with hydrochloric acid (40 mL, 0.5 N), and washed with ethyl acetate (20 mL*2). The aqueous phase was adjusted to the pH about 8 with sodium carbonate, and extracted with ethyl acetate (30 mL*2). The organic phase was concentrated to dryness by rotary evaporation, and the resulting residue was purified by preparative chromatography (dichloromethane/methanol=10/1) to obtain (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine (28 mg, yield: 27%) as a light yellow solid. 
       1 H NMR (400 MHz, MeOD) δ 7.61 (d, J=8 Hz, 1H), 5.99 (d, J=8 Hz, 1H), 5.36 (s, 1H), 4.31-4.13 (m, 2H), 3.45 (s, 1H), 3.30-3.15 (m, 2H), 2.43-2.24 (m, 2H), 1.89-1.48 (m, 5H), 0.84-0.65 (m, 2H), 0.57-0.36 (m, 2H). 
     MS m/z (ESI): 462.1 [M+H] + , 474.1 [M+2+H] + . 
     Example 57 
     Preparation of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-2-methyl-8-azaspiro[4.5]dec-2-en-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 57 was prepared by referring to the experimental scheme of Example 53. 
     MS m/z (ESI): 436.1 [M+H] + , 438.1[M+2+H] + . 
     Example 58 
     Preparation of (S)-(3-(1-amino-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-8-yl)-6-((2-amino-3-chloropyridin-4-yl)thio)-5-methylpyrazin-2-yl)methanol 
     
       
         
         
             
             
         
       
     
     The compound of Example 58 was prepared by referring to the experimental scheme of Example 45. 
     MS m/z (ESI): 473.1 [M+H] + , 475.1[M+2+H] + . 
     Example 59 
     Preparation of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     
       
         
         
             
             
         
       
     
     The compound of Example 59 was prepared by referring to the experimental scheme of Example 53. 
       1 H NMR (400 MHz, MeOD) δ 7.66-7.54 (m, 2H), 7.42-7.33 (m, 1H), 7.29-7.16 (m, 3H), 5.93 (d, J=5.5 Hz, 1H), 4.37-4.19 (m, 2H), 3.98 (s, 1H), 3.29-3.20 (m, 2H), 3.15 (d, J=16 Hz, 1H), 2.83 (d, J=16 Hz, 1H), 1.91-1.68 (m, 2H), 1.65-1.53 (m, 1H), 1.50-1.39 (m, 1H). 
     MS m/z (ESI): 454.1 [M+H] + , 456.1[M+2+H] + . 
     II. Biological Assay and Evaluation of the Compounds 
     The present invention is further described below in combination with the following test examples, which are not intended to limit the scope of the present invention. 
     Test Example 1. Determination of the Inhibitory Effect of the Compounds of the Present Invention on SHP-2 Kinase Activity 
     Experimental Objective: 
     The objective of this test is to determine the inhibitory effect of the compounds on SHP-2 full-length protein allosteric activity. 
     Experimental instruments: Centrifuge (5810R, purchased from Eppendorf), pipette (purchased from Eppendorf or Rainin), and microplate reader (Model: SynergyH1 full-function microplate reader, purchased from BioTek, USA). 
     Experimental method: Homogeneous Full Length SHP-2 Assay Kit (BPS Bioscience, #79330) was used to determine the in vitro SHP-2 activity. First, 18 μL of Master Mix was added to a 96-well low adsorption microplate (NUNC, #267342), i.e., the reaction buffer with a final concentration of 1× comprised 0.5 M SHP-2 activating Peptide and 5 mM DTT. After centrifugation, 5 μL of test compound/DMSO was added to each well (the final DMSO content was 1%, V/V, the test compound was dissolved in DMSO to 1 mM, a three-fold serial dilution was carried out to obtain ten concentrations, the final concentration of the reaction system ranged from 1 μM to 0.05 nM). SHP-2 was diluted in the 1× reaction buffer to a final concentration of 0.06 nM, and the resulting solution was added to the reaction microplate (2 μL per well). Full activity control (compound only with DMSO) and full inhibition control (no SHP-2) were set up on the reaction plate. After centrifugation, the reaction mixture was incubated at room temperature for 60 minutes. 
     After completion of the incubation, 25 μL of Substrate solution, which comprised Substrate with a final concentration of 10 μM and 5 mM DTT, was added to each well. After centrifugation, the reaction mixture was incubated at room temperature for 30 minutes. After completion of the reaction, readings were measured on the Synergy H1 full-function microplate reader (BioTek) (excitation wavelength: 340 nm, emission wavelength: 455 nM, gain value: 75). 
     Experimental Data Processing Method: 
     Based on the full activity control and full inhibition control used as the Max and Min values, the percentage inhibition rate data of the wells treated by the compounds was calculated through the positive control well (DMSO control well) and negative control well (no kinase) on the plate {% inhibition rate=100−[(test compound value−Min average)/(Max average−Min average)]×100}. The IC 50  value of the test compounds was calculated by fitting the percentage inhibition rate and data of ten concentrations to a four-parameter nonlinear logic formula with GraphPad prism. 
     Experimental Conclusion: 
     According to the above scheme, the compounds of the examples of the present invention showed the following biological activities in Table 2.1 in the SHP-2 kinase activity test. 
     
       
         
           
               
             
               
                 TABLE 2.1 
               
             
            
               
                   
               
               
                 Relative IC 50  value of the compounds  
               
               
                 on inhibiting SHP-2 kinase activity 
               
            
           
           
               
               
               
            
               
                   
                   
                 SHP-2 
               
               
                   
                 Example No. 
                 IC 50  (nM) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 Example 1 
                 17.8 
               
               
                   
                 Example 2 
                 4.17 
               
               
                   
                 Example 3 
                 4.33 
               
               
                   
                 Example 4 
                 11.7 
               
               
                   
                 Example 5 
                 6.27 
               
               
                   
                 Example 6 
                 9.28 
               
               
                   
                 Example 7 
                 13.2 
               
               
                   
                 Example 8 
                 3.93 
               
               
                   
                 Example 9 
                 29.6 
               
               
                   
                 Example 10 
                 0.98 
               
               
                   
                 Example 11 
                 5.22 
               
               
                   
                 Example 12 
                 3.75 
               
               
                   
                 Example 13 
                 0.66 
               
               
                   
                 Example 35 
                 3.80 
               
               
                   
                 Example 36 
                 1.48 
               
               
                   
                 Example 45 
                 6.81 
               
               
                   
                 Example 46 
                 1.73 
               
               
                   
                 Example 53 
                 1.75 
               
               
                   
                 Example 54 
                 9.29 
               
               
                   
                 Example 55 
                 3.07 
               
               
                   
                 Example 56 
                 3.78 
               
               
                   
                 Example 57 
                 4.25 
               
               
                   
                   
               
            
           
         
       
     
     The above data show that the compounds of the examples of the present invention have a good activity in inhibiting SHP-2 kinase activity. 
     Test Example 2. Determination of the Proliferation Inhibitory Activity of the Compounds of the Present Invention on KYSE520 Cells 
     The objective of this test example is to determine the proliferation inhibitory effect of the compounds of the present invention on tumor cells. The proliferation inhibitory activity of the compounds on tumor cells was determined by the CellTiter-Glo method, and the half inhibitory concentration IC 50  of the compounds on inhibiting cell proliferation was obtained. 2000 cells (90 μL of cell suspension) were inoculated to each well of a 96-well cell culture plate (Corning, #3610). The culture plate was incubated in an incubator overnight (37° C., 5% CO 2 ). On the next day, 10 μL of gradient dilutions of the test compound solution (the final DMSO content was 0.1%, V/V, the final concentration of the reaction system ranged from 100 μM to 45 nM) was added to each well of the culture plate. At the same time, full activity control (compound only with DMSO) and full inhibition control (no cells) were set up on the reaction plate. The culture plate was incubated in the incubator for 3 days (37° C., 5% CO 2 ). 
     After completion of the incubation, 50 μL of CellTiter-Glo reagent was added to each well of the cell culture plate, which was left to stand at room temperature for 10 minutes. The chemiluminescence signal value was measured on the Synergy H1 full-function microplate reader (BioTek). Based on the full activity control and full inhibition control used as the Max and Min values, the percentage inhibition rate data of the wells treated by the compounds was calculated {% inhibition rate=100−[(test compound value−Min average)/(Max average−Min average)]×100}. The IC 50  value of the test compounds was calculated by fitting the percentage inhibition rate and data of eight concentrations to a four-parameter nonlinear logic formula with GraphPad prism. 
     According to the above scheme, the compounds of the present invention showed a biological activity of about 0.1 nM to 200 nM (IC 50 ) in the proliferation inhibitory activity test on KYSE520 cells. 
     In some embodiments, the IC 50  of the compounds of the present invention on inhibiting the proliferation of KYSE520 cells was less than about 200 nM, preferably less than about 100 nM, further preferably less than about 10 nM, more preferably less than about 1 nM, and most preferably less than 1 nM. 
     Test Example 3. Determination of the Proliferation Inhibitory Activity of the Compounds of the Present Invention on NCI-11358 Cells 
     Experimental objective: The objective of this test example is to determine the proliferation inhibitory effect of the compounds of the present invention on tumor cells. 
     Experimental instruments: Centrifuge (5810R, purchased from Eppendorf), carbon dioxide incubator (purchased from Thermo), biological safety cabinet (purchased from Shanghai Boxun Company), pipette (purchased from Eppendorf or Rainin), and microplate reader (Model: SynergyH1 full-function microplate reader, purchased from BioTek, USA). 
     Experimental method: 3000 cells (90 μL of cell suspension) were inoculated to each well of a 96-well cell culture plate (Corning, #3610). The culture plate was incubated in an incubator overnight (37° C., 5% CO 2 ). On the next day, 10 μL of gradient dilutions of the test compound solution (the final DMSO content was 0.2%, V/V, the final concentration of the reaction system ranged from 10 μM to 4.5 nM) was added to each well of the culture plate. At the same time, full activity control (compound only with DMSO) and full inhibition control (no cells) were set up on the reaction plate. The culture plate was incubated in the incubator for 3 days (37° C., 5% CO 2 ). 
     After completion of the incubation, 50 μL of CellTiter-Glo reagent was added to each well of the cell culture plate, which was left to stand at room temperature for 10 minutes. The chemiluminescence signal value was measured in the Synergy H1 full-function microplate reader (BioTek). Based on the full activity control and full inhibition control used as the Max and Min values, the percentage inhibition rate data of the wells treated by the compounds was calculated {% inhibition rate=100−[(test compound value−Min average)/(Max average−Min average)]×100}. The IC 50  value of the test compounds was calculated by fitting the percentage inhibition rate and data of eight concentrations to a four-parameter nonlinear logic formula with GraphPad prism. 
     Experimental Data Processing Method: 
     The percentage inhibition data of the wells treated by the compounds of the examples was calculated through the vehicle control well on the plate {% inhibition rate=100−(test compound value/vehicle control value)×100}. The IC 50  value was calculated by fitting the data of different concentrations and corresponding percentage inhibition rates to a four-parameter nonlinear logic formula with GraphPad prism. 
     Experimental Conclusion: 
     According to the above scheme, the compounds of the examples of the present invention showed the following biological activities in Table 2.2 in the NCI-H358 cell proliferation inhibition test. 
     
       
         
           
               
             
               
                 TABLE 2.2 
               
             
            
               
                   
               
               
                 IC 50  value of the compounds on inhibiting  
               
               
                 the proliferation of NCI-H358 cells 
               
            
           
           
               
               
               
            
               
                   
                   
                 NCI-H358 
               
               
                   
                 Example No. 
                 IC 50  (nM) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 Example 1 
                 37.0 
               
               
                   
                 Example 2 
                 10.5 
               
               
                   
                 Example 3 
                 31.1 
               
               
                   
                 Example 4 
                 184 
               
               
                   
                 Example 5 
                 6.39 
               
               
                   
                 Example 6 
                 13.4 
               
               
                   
                 Example 7 
                 41.87 
               
               
                   
                 Example 8 
                 18.5 
               
               
                   
                 Example 9 
                 32.8 
               
               
                   
                 Example 10 
                 24.5 
               
               
                   
                 Example 11 
                 37.7 
               
               
                   
                 Example 12 
                 42.6 
               
               
                   
                 Example 13 
                 47.0 
               
               
                   
                 Example 35 
                 23.3 
               
               
                   
                 Example 36 
                 9.19 
               
               
                   
                 Example 45 
                 25.9 
               
               
                   
                 Example 46 
                 11.9 
               
               
                   
                 Example 53 
                 20.9 
               
               
                   
                 Example 54 
                 79.9 
               
               
                   
                 Example 55 
                 46.5 
               
               
                   
                 Example 56 
                 78.5 
               
               
                   
                 Example 57 
                 56.5 
               
               
                   
                   
               
            
           
         
       
     
     The above data show that the compounds of the examples of the present invention have a good activity in inhibiting the proliferation of NCI-H358 cells. 
     Test Example 4. Pharmacokinetics Assay in Mice 
     4.1. Study Objective: 
     Balb/c mice were used as test animals. The pharmacokinetic behavior of the compounds of Examples 36, 46, 53, 54 and 55 after oral administration in mouse body (plasma) was studied. 
     4.2. Test Protocol 
     4.2.1 Test Compounds: 
     Compounds of Examples 36, 46, 53, 54 and 55, self-prepared; 
     4.2.2 Test Animals: 
     Male Balb/c mice were purchased from Shanghai Jiesijie Laboratory Animal Co., LTD, with Certificate No.: SCXK (Shanghai) 2013-0006 N0.311620400001794. 
     4.2.3 Preparation of the Test Compounds: 
     5 g of hydroxyethyl cellulose (HEC, CMC-Na, viscosity: 800-1200 Cps) was dissolved in 1000 mL of purified water, and 10 g of Tween 80 was added. The solution was mixed well to form a clear solution. 
     1.2 mg of the compounds of Examples 36, 46, 53, 54 and 55 were respectively added to a 4-mL glass bottle, followed by the addition of 2.4 mL of the above solution. The solution was subjected to ultrasound for 10 minutes to obtain a colorless and clear solution with a concentration of 0.5 mg/mL. 
     4.2.4 Administration: 
     After an overnight fast, male Balb/c mice were administrated orally with the test compounds at an administration dose of 5 mg/kg and an administration volume of 10 mL/kg. 
     4.2.5 Sample Collection: 
     Blood was taken before administration and at 0.25 hour, 0.5 hour, 1 hour, 2 hours, 4 hours, 6 hours, 8 hours and 24 hours after administration. The blood samples were stored in EDTA-2K tubes, and centrifuged for 6 minutes at 6000 rpm, at 4° C. to separate the blood plasma. The plasma samples were stored at −80° C. The mice were fed 4 hours after administration. 
     4.2.6 Test Results: 
     The test results were determined by LCMS/MS method as shown in Table 2.3. 
     
       
         
           
               
             
               
                 TABLE 2.3 
               
             
            
               
                   
               
               
                 Pharmacokinetic parameters of the compounds in mice 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Tmax 
                 Cmax 
                 AUC 0-∞   
                 T 1/2   
                 MRT 
               
               
                 Example No. 
                 (hr) 
                 (ng/mL) 
                 (ng/mL * hr) 
                 (hr) 
                 (hr) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Example 36 
                 1.0 
                 436.0 
                 5390.0 
                 NA 
                 9.08 
               
               
                 Example 46 
                 0.5 
                 474.7 
                 2214.7 
                 3.07 
                 4.34 
               
               
                 Example 53 
                 0.5 
                 179.0 
                 2441.8 
                 5.23 
                 7.92 
               
               
                 Example 54 
                 0.5 
                 996.3 
                 5976.9 
                 3.92 
                 5.15 
               
               
                 Example 55 
                 0.5 
                 1383.3 
                 8156.7 
                 4.63 
                 5.07 
               
               
                   
               
            
           
         
       
     
     The above data show that the compounds of the examples of the present invention have a good metabolic activity at a dose of 5 mg/kg. 
     Test Example 5. Pharmacokinetics Assay in Rats 
     5.1. Study Objective: 
     SD rats were used as test animals. The pharmacokinetic behavior of the compounds of Examples 36, 46, 53, 54, 55 and 59 after oral administration in rat body (plasma) was studied. 
     5.2. Test Protocol 
     5.2.1 Test Compounds: 
     Compounds of Examples 36, 46, 53, 54, 55 and 59, self-prepared; 
     5.2.2 Test Animals: 
     Male SD rats (3 rats per group) were purchased from Shanghai Jiesijie Laboratory Animal Co., LTD, with Certificate No.: SCXK (Shanghai) 2013-0006 N0.311620400001794. 
     5.2.3 Preparation of the Test Compounds: 
     5 g of hydroxyethyl cellulose (HEC, CMC-Na, viscosity: 800-1200 Cps) was dissolved in 1000 mL of purified water, and 10 g of Tween 80 was added. The solution was mixed well to form a clear solution. 
     3.9 mg of the compounds of Examples 36, 46, 53, 54, 55 and 59 were respectively dissolved in the above solution. The solution was shaked well and subjected to ultrasound for 15 minutes to obtain a colorless and clear solution with a concentration of 0.5 mg/mL. 
     5.2.4 Administration: 
     After an overnight fast, male SD rats (3 rats per group) were administrated orally with the test compounds at an administration dose of 5 mg/kg and an administration volume of 10 mL/kg. 
     5.2.5 Sample Collection: 
     0.2 mL of blood was taken from jugular vein before administration and at 0.5 hour, 1.0 hour, 2.0 hours, 4.0 hours, 6.0 hours, 8.0 hours and 24.0 hours after administration. The blood samples were stored in EDTA-2K tubes, and centrifuged for 6 minutes at 6000 rpm, at 4° C. to separate the blood plasma. The plasma samples were stored at −80° C. The rats were fed 4 hours after administration. 
     5.3 Test results: The test results were determined by LCMS/MS method as shown in Table 2.4. 
     
       
         
           
               
             
               
                 TABLE 2.4 
               
             
            
               
                   
               
               
                 Pharmacokinetic parameters of the  
               
               
                 compounds of the present invention in rats 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Tmax 
                 Cmax 
                 AUC 0-∞   
                 T 1/2   
                 MRT 
               
               
                 Example No. 
                 (hr) 
                 (ng/mL) 
                 (ng/mL * hr) 
                 (hr) 
                 (hr) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Example 36 
                 1.0 
                 247.3 
                 3305.3 
                 6.49 
                 9.24 
               
               
                 Example 46 
                 1.0 
                 189.0 
                 1783.0 
                 3.69 
                 5.78 
               
               
                 Example 53 
                 2.0 
                 201.0 
                 3842.0 
                 10.51 
                 15.15 
               
               
                 Example 54 
                 1.0 
                 666.3 
                 6252.6 
                 4.12 
                 5.67 
               
               
                 Example 55 
                 2.0 
                 614.3 
                 5891.6 
                 3.55 
                 5.84 
               
               
                 Example 59 
                 2.0 
                 113.8 
                 1537.4 
                 5.11 
                 7.52 
               
               
                   
               
            
           
         
       
     
     Experimental Conclusion: The data in the table show that the compounds of the examples of the present invention reached a relatively high exposure in rat plasma after oral administration at a dose of 5 mg/kg. In addition, perianal pollution was observed in rats administered with the compound of Example 59, indicating that the compound of Example 59 has a gastrointestinal toxicity. 
     Test Example 6. Tumor Inhibition Test on MiaPaca 2 Xenograft Model 
     6.1 Experimental Objective: 
     Balb/c nude mice were used as test animals. In vivo pharmacodynamic test was carried out on human pancreatic cancer cell MiaPaca 2 xenograft (CDX) model to evaluate the anti-tumor effect of the test compounds. 
     6.2 Experimental Instruments and Reagents: 
     6.2.1 Instruments: 
     Ultra-clean workbench (BSC-130011 A2, Shanghai Boxun Medical Biological Instrument Corp.) 
     CO 2  incubator (Thermo-311, Thermo) 
     Centrifuge (Centrifuge 5720R, Eppendorf) 
     Automatic cell counter (Countess II, Life Technologies) 
     Pipette (10-20 μL, Eppendorf) 
     Microscope (Ts 2, Nikon) 
     Vernier caliper (CD-6″ AX, Mitutoyo, Japan) 
     Cell culture flask (T25/T75/T225, Corning) 
     Constant temperature water tank (HWS12, Shanghai YIHENG Technical Co., Ltd.) 
     6.2.2 Reagents: 
     DMEM (11995-065, Gibco) 
     Fetal Bovine Serum (FBS) (10091-148, Gibco) 
     0.25% Trypsin (25200-056, Gibco) 
     Penicillin-streptomycin double antibiotics (P/S) (SV30010, GE) 
     Phosphate buffered saline (PBS) (10010-023, Gibco) 
     Matrigel (356234, Corning) 
     Gln (25030-081, Gibco) 
     6.3 Experimental Process: 
     MiaPaca 2 cells were taken from the cell bank and thawed. The cells were added to the DMEM medium (containing 10% FBS, 1% Glu and 1% P/S), and incubated in the CO 2  incubator (the temperature of the incubator was 37° C., and the CO 2  concentration was 5%). After the cells covered 80-90% of the bottom of the culture flask, the cells were passaged. After passage, the cells continued to be incubated in the CO 2  incubator. The process was repeated until the number of cells met the inoculation requirement of the in vivo pharmacodynamic test. The cells in logarithmic growth phase were collected, and counted by the automatic cell counter. According to the counting results, the cells were resuspended with PBS and Matrigel (volume ratio: 1:1) to obtain a cell suspension (density: 8×10 7 /ml), which was placed in an ice box for later use. 
     Female Balb/c nude mice (6-8 weeks old, body weight: about 18-22 grams) were used as test animals. The mice were maintained in an environment free of special pathogens and in a single ventilated cage (five mice per cage). All cages, bedding and water were disinfected before use. All animals had free access to standard certified commercial laboratory animal diets. The nude mice were marked with a disposable universal ear tag for rats and mice before the test. The skin of the inoculation site was disinfected with 75% medical alcohol before the inoculation. Each mouse was inoculated with 0.1 ml of MiaPaca 2 tumor cells (containing 8*10 6  cells) subcutaneously on the right back. When the average tumor volume reached 100 to 200 mm 3 , administration was carried out. The test compounds were administered intragastrically daily. The dose, frequency of administration and the efficacy of each group at the end of the test are shown in Table 5. The tumor volume (mm 3 ) was measured with the vernier caliper twice a week. The tumor volume calculation formula: V=0.5*D*d*d, wherein D and d represent the long diameter and short diameter of the tumor, respectively. The anti-tumor efficacy was determined by dividing the average tumor increase volume of the compound-treated animals by the average tumor increase volume of the untreated animals. Tumor growth inhibition rate calculation formula: TGI (%)=1−[(Vt−V0) administration group/(Vt−V0) vehicle control group]*100%. All animals were euthanized after the experiment. 
     6.4 Experimental Results: 
     
       
         
           
               
             
               
                 TABLE 2.5 
               
             
            
               
                   
               
               
                 Pharmacodynamic parameters of the compounds in mice with xenograft 
               
            
           
           
               
               
               
               
            
               
                   
                 Tumor volume 
                 ΔT/ΔC 
                 TGI 
               
               
                   
                 (mm 3 , Mean ± SD) 
                 (%) 
                 (%) 
               
            
           
           
               
               
               
               
               
            
               
                 Group 
                 Day 0 
                 Day 21 
                 Day 21 
                 Day 21 
               
               
                   
               
               
                 Example 6 
                 170 ± 28 
                 183 ± 70 
                 2.24 
                 97.76 
               
               
                 6 mg/kg QD × 3w 
                 (171 ± 25) 
                  (780 ± 248) 
                   
                   
               
               
                 Example 35 
                 166 ± 27 
                 196 ± 62 
                 4.36 
                 95.64 
               
               
                 6 mg/kg QD × 3w 
                 (168 ± 32) 
                  (847 ± 287) 
                   
                   
               
               
                 Example 36 
                 172 ± 26 
                 216 ± 66 
                 4.93 
                 95.07 
               
               
                 3 mg/kg QD × 3w 
                 (173 ± 28) 
                 (1064 ± 236) 
                   
                   
               
               
                 Example 46 
                 167 ± 26 
                 198 ± 70 
                 4.57 
                 95.43 
               
               
                 6 mg/kg QD × 3w 
                 (168 ± 32) 
                  (847 ± 287) 
                   
                   
               
               
                 Example 53 
                 166 ± 28 
                 227 ± 37 
                 9.00 
                 91.00 
               
               
                 6 mg/kg QD × 3w 
                 (168 ± 32) 
                  (847 ± 287) 
                   
                   
               
               
                 Example 55 
                 176 ± 36 
                 240 ± 86 
                 7.06 
                 92.94 
               
               
                 10 mg/kg QD × 3w 
                 (176 ± 39) 
                 (1078 ± 395) 
               
               
                   
               
               
                 Note: 
               
               
                 The data in parentheses refers to the tumor volume of the Vehicle QD × 3w group (i.e. the control group) corresponding to this Example at the corresponding time (i.e. Day 0 and Day 21, respectively). 
               
            
           
         
       
     
     Experimental Conclusion: The above data show that after 21 days of continuous oral administration, the compounds of the examples of the present invention can significantly inhibit the growth of MiaPaca 2 xenograft in nude mice under the administration condition of 3 to 10 mg/kg QD. 
     Test Example 7. Pharmacokinetics Assay in Tumor-Bearing Mice 
     7.1. Study Objective: 
     MiaPaca 2 tumor-bearing mice were used as test animals. The pharmacokinetic behavior of the compound of Example 53 after oral administration at a dose of 6 mg/kg in mouse body (plasma, tumor tissue and intestine) was studied. 
     7.2. Test Protocol 
     7.2.1 Test Compound: 
     Compound of Example 53, self-prepared. 
     7.2.2 Test Animals: 
     Twenty-four female MiaPaca 2 tumor-bearing mice were used. Three mice were used for each time point (0 hour, 1 hour, 2 hours, 4 hours, 6 hours, 8 hours, 16 hours and 24 hours). The mice were purchased from Shanghai Sippr-BK laboratory animal Co. Ltd., with Certificate No.: SCXK (Shanghai) 2018-0006. 
     7.2.3 Preparation of the Test Compound: 
     5 g of hydroxymethyl cellulose was dissolved in 1000 mL of purified water, and 10 g of Tween 80 was added. The solution was mixed well to form a clear solution. 
     4.57 mg of the compound of Example 53, 22.6 mg was dissolved in the above solution. The solution was shaked well, and subjected to ultrasound for 15 minutes to obtain a uniform suspension with a concentration of 0.6 mg/mL. 
     7.2.4 Administration: 
     After fast, MiaPaca 2 tumor-bearing mice were administered orally (p.o.) with the test compound according to body weight at an administration dose of 6 mg/kg and an administration volume of 10 mL/kg (the animals were not administered with the test compound on 0 hour). 
     7.2.5 Sample Collection: 
     The mice were euthanized by CO 2  before administration and after administration, and 0.5 mL of blood was taken from heart. The blood samples were stored in EDTA-2K tubes, and centrifuged for 6 minutes at 6000 rpm, at 4° C. to separate the blood plasma. The plasma samples were stored at −80° C. The tumor tissue was weighed, placed in a 2 mL centrifuge tube, and stored at 80° C. An appropriate length of duodenum, ileum and colon tissues were taken and cut open with scissors, and the contents were removed. The above tissues were washed with PBS twice, and the moisture was absorbed with absorbent paper. The tissues were weighed, placed in a 2 mL centrifuge tube, and stored at −80° C. 
     7.3 Test results: The test results were determined by LCMS/MS method as shown in Table 2.6. 
     
       
         
           
               
             
               
                 TABLE 2.6 
               
             
            
               
                   
               
               
                 Pharmacokinetic parameters of the compound 
               
               
                 of the present invention in mice 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Tmax 
                 Cmax 
                 AUC 0-∞   
                 T 1/2   
                 MRT 
               
               
                 Compound No. 
                 (hr) 
                 (ng/mL) 
                 (ng/mL*hr) 
                 (hr) 
                 (hr) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Example 
                 Plasma 
                 1.0 
                 385.7 
                 2857.0 
                 4.8 
                 7.5 
               
               
                 53 
                 Tumor 
                 8.0 
                 2388.3 
                 51774.2 
                 11.5 
                 19.1 
               
               
                   
                 Duodenum 
                 1.0 
                 5416.7 
                 16252.0 
                 4.8 
                 4.8 
               
               
                   
                 Ileum 
                 2.0 
                 1928.3 
                 10008.3 
                 5.3 
                 6.1 
               
               
                   
                 Colon 
                 4.0 
                 1310.0 
                 9182.5 
                 2.8 
                 5.8 
               
               
                   
               
            
           
         
       
     
     Experimental Conclusion: The data in the table show that at a dose of 6 mg/kg, the exposure of the compound of the example of the present invention in mice tumor reached a very high level, and the exposure in tumor was significantly higher than that in blood. It can be seen from Tmax and MRT that the concentration of the compound in tumor gradually increased and the TI/2 was longer, indicating that the compound will gradually accumulate in tumor and always maintain a higher concentration in tumor, thereby ensuring a better tumor inhibition effect. 
     Test Example 8. Test of Inhibitory Activity on hERG Potassium Channel 
     8.1 Cell Preparation 
     8.1.1 CHO-hERG cells were cultured in a 175 cm 2  culture flask. When the cells were grown to a density of 60 to 80%, the culture solution was removed, and the cells were washed once with 7 mL of PBS, and then 3 mL of Detachin was added for digestion of the cells. 
     8.1.2 After the digestion was complete, the cells were neutralized by adding 7 mL of the culture solution and then centrifuged. The supernatant was removed, and then 5 mL of the culture solution was added for resuspension to ensure a cell density of 2 to 5×10 6  cells/mL. 
     8.2 Solution Preparation 
     
       
         
           
               
             
               
                 TABLE 2.7 
               
             
            
               
                   
               
               
                 Components of intracellular fluid and extracellular fluid 
               
            
           
           
               
               
               
            
               
                 Reagent 
                 Extracellular fluid (mM) 
                 Intracellular fluid (mM) 
               
               
                   
               
            
           
           
               
               
               
            
               
                 CaCl 2   
                 2 
                 5.374 
               
               
                 MgCl 2   
                 1 
                 1.75 
               
               
                 KCl 
                 4 
                 120 
               
               
                 NaCl 
                 145 
                 — 
               
               
                 Glucose 
                 10 
                 — 
               
               
                 HEPES 
                 10 
                 10 
               
               
                 EGTA 
                 — 
                 5 
               
               
                 Na-ATP 
                 — 
                 4 
               
               
                 pH 
                 7.40 (adjusted with NaOH), 
                 7.25 (adjusted with KOH), 
               
               
                   
                 Osmolarity~305 mOsm 
                 Osmolarity~290 mOsm 
               
               
                   
               
            
           
         
       
     
     8.3 Electrophysiological Recording Process 
     The single-cell high-impedance sealing and whole-cell pattern formation processes were both completed automatically by the Qpatch instrument. After the whole-cell recording pattern was obtained, the cells were clamped at −80 millivolts. A pre-voltage of −50 millivolts was applied for 50 milliseconds prior to the application of a depolarizing stimulus of ±40 millivolts for 5 seconds, and then the cells were repolarized to −50 millivolts and maintained for 5 seconds, and then returned back to −80 millivolts. This voltage stimulation was applied every 15 seconds, and data were recorded for 2 minutes, and thereafter the extracellular fluid was administered and data were recorded for 5 minutes, and then the administration process was started. The compound was administered from the lowest test concentration, and 2.5 minutes were given for each test concentration. After all the concentrations of the compound were administered, 3 M Cisapride as a positive control compound was administered. At least three cells (n≥3) were tested for each concentration. 
     8.4 Compound Preparation 
     8.4.1 20 mM compound mother solution was diluted with the extracellular fluid. 5 μL of the 20 mM compound mother solution was diluted 500 times to 40 μM by adding 2495 μL of the extracellular fluid, and then 3-fold serial dilutions were performed sequentially in the extracellular fluid containing 0.2% DMSO to obtain a final concentration to be tested. 
     8.4.2 The highest test concentration was 40 μM, and there were six concentrations in total, i.e., 40, 13.33, 4.44, 1.48, 0.49, and 0.16 μM, respectively. 
     8.4.3 The DMSO content in the final test concentration was not more than 0.2%. This concentration of DMSO had no effect on the hERG potassium channel. 
     8.5 Data Analysis 
     The experimental data were analyzed by XLFit software. 
     8.6 Quality Control 
     Environment: Humidity 20 to 50% and temperature 22 to 25° C. 
     Reagents: The experimental reagents used were purchased from Sigma, with a purity &gt;98%. 
     The experimental data in the report must meet the following criteria: 
     Whole cell sealing impedance &gt;100 MΩ 
     Tail current amplitude &gt;400 pA 
     Pharmacological parameters: 
     The inhibitory effect of multiple concentrations of Cisapride on hERG channel was set as a positive control. 
     8.7 Experimental Results 
     The inhibitory effect of the compounds of the examples at multiple concentrations on hERG current is as follows: 
     
       
         
           
               
             
               
                 TABLE 2.8 
               
             
            
               
                   
               
               
                 Inhibitory effect of the compounds of the examples  
               
               
                 at multiple concentrations on hERG current 
               
            
           
           
               
               
               
            
               
                   
                 Example No. 
                 hERG (uM) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 Example 6 
                 5.24 
               
               
                   
                 Example 35 
                 6.17 
               
               
                   
                 Example 36 
                 2.34 
               
               
                   
                 Example 46 
                 18.78 
               
               
                   
                 Example 53 
                 4.51 
               
               
                   
                 Example 55 
                 21.91 
               
               
                   
                   
               
            
           
         
       
     
     Experimental Conclusion: The inhibition of a drug on the cardiac hERG potassium channel is the main reason that the drug causes QT prolongation syndrome. It can be seen from the experimental results that the compounds of the examples of the present invention have no obvious inhibitory effect on cardiac hERG potassium channel, thereby avoiding cardiotoxic side effects at high doses. 
     Test Example 9. Pharmacokinetics Assay in Mice 
     9.1. Study Objective: 
     Balb/c mice were used as test animals. The pharmacokinetic behavior of the compounds of Examples 53 and 59 after single intravenous injection in mouse body (plasma) was studied. 
     9.2. Test Protocol 
     9.2.1 Test Compounds: 
     Compounds of Examples 53 and 59, self-prepared; 
     9.2.2 Test Animals: 
     Male Balb/c mice were purchased from Shanghai Jiesijie Laboratory Animal Co., LTD, with Certificate No.: SCXK (Shanghai) 2013-0006 N0.311620400001794. 
     9.2.3 Preparation of the Test Compounds: 
     Aqueous solution containing 10% Solutol HS15: 10 g of Solutol HS15 was added to purified water for injection, and dissolved in it under stirring. The solution was diluted to 100 mL with purified water for injection and shaked well to obtain the desired solution. 
     Aqueous solution containing 20% HP-β-CD: 20 g of HP-β-CD was added to purified water for injection, and dissolved in it under stirring. The solution was diluted to 100 mL with purified water for injection and shaked well to obtain the desired solution. 
     0.5 ml of DMSO, 8.5 ml of 20% HP-β-CD aqueous solution and 1 ml of 10% Solutol HS15 aqueous solution were mixed and shaked well to obtain a clear solution. 
     0.234 mg of the compounds of Examples 53 and 59 were weighed and added to 1.17 mL of the above solution. The solution was subjected to ultrasound to obtain a clear solution. The solution was filtered with a 0.22 m water filter, and the filtrate was the formulation for intravenous administration (i.v.). The concentration was 0.2 mg/mL. 
     9.2.4 Administration: 
     After an overnight fast, male Balb/c mice were administered intravenously with the test compounds at an administration dose of 1 mg/kg and an administration volume of 5 mL/kg. 
     9.2.5 Sample Collection: 
     Blood was taken before administration and at 0 minute, 5 minutes, 15 minutes, 0.5 hour, 1.0 hour, 2.0 hours, 4.0 hours, 8.0 hours and 24.0 hours after administration. The blood samples were stored in EDTA-2K tubes, and centrifuged for 6 minutes at 6000 rpm, at 4° C. to separate the blood plasma. The plasma samples were stored at −80° C. The mice were fed 4 hours after administration. 
     9.3 Test results: The test results were determined by LCMS/MS method as shown in Table 2.9. 
     
       
         
           
               
             
               
                 TABLE 2.9 
               
             
            
               
                   
               
               
                 Pharmacokinetic parameters of the compounds in mice 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 C 0   
                 AUC 0-∞   
                   
                   
                 CL 
                 Vss 
                   
               
               
                   
                 (ng/ 
                 (ng/ 
                 T 1/2   
                 MRT 
                 (mL/ 
                 (L/ 
                 F 
               
               
                 Example No. 
                 mL) 
                 mL*hr) 
                 (hr) 
                 (hr) 
                 min/kg) 
                 kg) 
                 (%) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Example 53 
                 264.5 
                 736.0 
                 7.74 
                 9.04 
                 22.6 
                 12.3 
                 71 
               
               
                 Example 59 
                 257.4 
                 404.0 
                 5.81 
                 5.96 
                 41.3 
                 14.7 
                 150 
               
               
                   
               
            
           
         
       
     
     Experimental conclusion: The compounds of the examples of the present invention have good metabolic characteristics in mouse body at a dose of 1 mg/kg. 
     Test Example 10. Pharmacokinetics Assay in Rats 
     10.1. Study Objective: 
     SD rats were used as test animals. The pharmacokinetic behavior of the compounds of Examples 53 and 59 after single intravenous injection in rat body (plasma) was studied. 
     10.2. Test Protocol 
     10.2.1 Test Compounds: 
     Compounds of Examples 53 and 59, self-prepared. 
     10.2.2 Test Animals: 
     Male SD rats (3 rats per group) were purchased from Shanghai Jiesijie Laboratory Animal Co., LTD, with Certificate No.: SCXK (Shanghai) 2013-0006 N0.311620400001794. 
     10.2.3 Preparation of the Test Compounds: 
     Aqueous solution containing 10% Solutol HS15: 10 g of Solutol HS15 was added to purified water for injection, and dissolved in it under stirring. The solution was diluted to 100 mL with purified water for injection and shaked well to obtain the desired solution. 
     Aqueous solution containing 20% HP-β-CD: 20 g of HP-β-CD was added to purified water for injection, and dissolved in it under stirring. The solution was diluted to 100 mL with purified water for injection and shaked well to obtain the desired solution. 
     0.5 ml of DMSO, 8.5 ml of 20% HP-β-CD aqueous solution and 1 ml of 10% Solutol HS15 aqueous solution were mixed and shaked well to obtain a clear solution. 
     0.78 mg of the compounds of Examples 53 and 59 were weighed and added to 3.9 mL of the above solution. The solution was subjected to ultrasound to obtain a clear solution. The solution was filtered with a 0.22 m water filter, and the filtrate was the formulation for intravenous administration (i.v.). The concentration was 0.2 mg/mL. 
     10.2.4 Administration: 
     After an overnight fast, male SD rats (3 rats per group) were administered intravenously with the test compounds at an administration dose of 1 mg/kg and an administration volume of 5 mL/kg. 
     10.2.5 Sample Collection: 
     0.2 mL of blood was taken from jugular vein before administration and at 0 minute, 5 minutes, 15 minutes, 0.5 hour, 1.0 hour, 2.0 hours, 4.0 hours, 8.0 hours and 24.0 hours after administration. The blood samples were stored in EDTA-2K tubes, and centrifuged for 6 minutes at 6000 rpm, at 4° C. to separate the blood plasma. The plasma samples were stored at −80° C. The rats were fed 4 hours after administration. 
     10.3 Test results: The test results were determined by LCMS/MS method as shown in Table 2.10. 
     
       
         
           
               
             
               
                 TABLE 2.10 
               
             
            
               
                   
               
               
                 Pharmacokinetic parameters of the compounds 
               
               
                 of the present invention in rats 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 C 0   
                 AUC 0-∞   
                   
                   
                 CL 
                 Vss 
                   
               
               
                   
                 (ng/ 
                 (ng/ 
                 T 1/2   
                 MRT 
                 (mL/ 
                 (L/ 
                 F 
               
               
                 Example No. 
                 mL) 
                 mL*hr) 
                 (hr) 
                 (hr) 
                 min/kg) 
                 kg) 
                 (%) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Example 53 
                 311.1 
                 612.6 
                 3.35 
                 4.26 
                 27.2 
                 7.0 
                 118 
               
               
                 Example 59 
                 516.5 
                 427.6 
                 1.83 
                 1.79 
                 39.0 
                 4.2 
                 72 
               
               
                   
               
            
           
         
       
     
     Experimental conclusion: The compounds of the examples of the present invention have good metabolic characteristics in rat body at a dose of 1 mg/kg. 
     Test Example 11. Tolerance Assay in Mice 
     11.1. Study Objective: 
     Balb/c mice were used as test animals. The tolerance of the compounds of Examples 53 and 59 after oral administration in female and male mice was studied. 
     11.2. Test Protocol 
     11.2.1 Test Compounds: 
     Compounds of Examples 53 and 59, self-prepared; 
     11.2.2 Test Animals: 
     Balb/c mice (9 male and 9 female) were purchased from Shanghai Jiesijie Laboratory Animal Co., LTD, with Certificate No.: SCXK (Shanghai) 2013-0006 N0.311620400001794. 
     11.2.3 Preparation of the Test Compounds: 
     5 g of hydroxyethyl cellulose (HEC, CMC-Na, viscosity: 800-1200 Cps) was dissolved in 1000 mL of purified water, and 10 g of Tween 80 was added. The solution was mixed well to form a clear solution. 
     41.0 mg of the compound of Example 53 and 25.9 mg of the compound of Example 59 were respectively added to a 100-mL glass bottle, followed by the addition of 41.0 mL and 25.9 mL of the above solution. The solution was subjected to ultrasound for 10 minutes to obtain a colorless and clear solution with a concentration of 1 mg/mL. 
     11.2.4 Administration: 
     After an overnight fast, Balb/c mice (3 male and 3 female) were administrated orally with the test compound at an administration dose of 10 mg/kg and an administration volume of 10 mL/kg. 
     11.2.5 Data Collection: 
     The mice were weighed daily before and after administration, and abnormal responses were observed. The mice were fed 4 hours after administration. 
     11.2.6 Test Results: 
     The body weight was weighed with a balance, and the test results were shown in Table 2.11. 
     
       
         
           
               
             
               
                 TABLE 2.11 
               
             
            
               
                   
               
               
                 Comparison of mouse body weight changes 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 Body weight 
               
               
                   
                   
                   
                 changes 
               
               
                   
                   
                 Body weight 
                 ΔW/W1  
               
               
                   
                   
                 (g, Mean) 
                 (%, Mean) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Group 
                 Day 1 
                 Day 16 
                 Day 16 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 Control group  
                 22.99 
                 27.34 
                 18.98 
               
               
                   
                 QD male 
                   
                   
                   
               
               
                   
                 Example 53 
                 23.01 
                 28.30 
                 23.25 
               
               
                   
                 10 mg/kg QD 
                   
                   
                   
               
               
                   
                 male 
                   
                   
                   
               
               
                   
                 Example 59 
                 23.03 
                 21.57 
                 −8.02 
               
               
                   
                 10 mg/kg QD 
                   
                   
                   
               
               
                   
                 male 
                   
                   
                   
               
               
                   
                 Control group  
                 22.00 
                 23.77 
                 7.99 
               
               
                   
                 QD female 
                   
                   
                   
               
               
                   
                 Example 53 
                 22.07 
                 25.09 
                 13.80 
               
               
                   
                 10 mg/kg QD 
                   
                   
                   
               
               
                   
                 female 
                   
                   
                   
               
               
                   
                 Example 59 
                 22.10 
                 18.74 
                 −15.32 
               
               
                   
                 10 mg/kg QD 
                   
                   
                   
               
               
                   
                 female 
               
               
                   
                   
               
            
           
         
       
     
     Experimental Conclusion: The above data show that the compounds of the examples of the present invention have a good tolerance in mice at a dose of 10 mg/kg. However, the mice administered with the compound of Example 59 showed loose stools, blood in the stool, significant weight loss and the like, indicating that this compound has a poor tolerance. 
     In summary, the present invention provides a series of highly active and selective SHP2 kinase inhibitors with novel structures. These inhibitors show good pharmacokinetic characteristics in both rats and mice, and also show good efficacy on Miapaca2 tumor-bearing mouse model. These inhibitors have great potential to be developed into single drugs or combination drugs for treating a tumor disease. 
     III. Study on the Crystal Forms of (S)-8-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     1.1 Experimental Instruments 
     1.1.1 Some Parameters of Physical and Chemical Testing Instruments 
     
       
         
           
               
               
               
             
               
                   
               
             
            
               
                 XRPD 
                 Instrument model 
                 BRUKER D8 ADVANCE 
               
               
                   
                 Diffraction ray 
                 CuK (40 kV, 25 mA) 
               
               
                   
                 Scan rate 
                 0.02°/S (2θ value) 
               
               
                   
                 Scan range 
                 4° to 40° (2θ value) 
               
               
                 DSC 
                 Instrument model 
                 NETZSCH DSC 214 polyma 
               
               
                   
                 Purge gas 
                 Nitrogen 
               
               
                   
                 Purge rate 
                 40 mL/min 
               
               
                   
                 Heating rate 
                 10° C./min 
               
               
                   
                 Temperature range 
                 25 to 350° C. 
               
               
                   
                 Plate type 
                 Aluminum plate 
               
               
                 TGA 
                 Instrument model 
                 NETZSCH TG 209 Tarsus 
               
               
                   
                 Purge gas 
                 Nitrogen 
               
               
                   
                 Purge rate 
                 40 mL/min 
               
               
                   
                 Heating rate 
                 10° C./min 
               
               
                   
                 Temperature range 
                 Room temperature~400° C. 
               
               
                   
                 Plate type 
                 Al 2 O 3   
               
               
                   
               
            
           
         
       
     
     1.2 Instruments and Liquid Chromatography Conditions 
     1.2.1 Instruments and Devices 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 Instrument name 
                 Model 
               
               
                   
                   
               
             
            
               
                   
                 Analytical Balances 
                 Sartorius BSA224S-CW 
               
               
                   
                 Water purifier 
                 Milli-Q Plus, Millipore 
               
               
                   
                 High performance liquid 
                 Agilent1260 
               
               
                   
                 chromatography 
                   
               
               
                   
                 Pump 
                 Agilent G1311B 
               
               
                   
                 Injector 
                 G1329B 
               
               
                   
                 Column oven 
                 G1316A 
               
               
                   
                 Detector 
                 G1315D 
               
               
                   
                   
               
            
           
         
       
     
     1.2.2 Chromatographic Conditions 
     Column: Waters Xbridge C18 (4.6 mm*150 mm, 3.5 μm) 
     Flow rate: 1 mL/min 
     Column temperature: 40° C. 
     Detection wavelength: 275 nm 
     Injection volume: 5.0 μL 
     Running time: 30 min 
     Diluent: DMSO 
     Mobile phase: A: water (0.05% trifluoroacetic acid); B: acetonitrile (0.05% trifluoroacetic acid) 
     
       
         
           
               
               
               
             
               
                   
               
               
                 T (min) 
                 A (%) 
                 B (%) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 0.00 
                 90 
                 10 
               
               
                 15.00 
                 70 
                 30 
               
               
                 25.00 
                 30 
                 70 
               
               
                 25.01 
                 90 
                 10 
               
               
                 30.00 
                 90 
                 10 
               
               
                   
               
            
           
         
       
     
     2. Preparation of Various Crystal Forms of (S)-8-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     2.1 Preparation of Crystal Form A of (S)-8-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     5 g of (S)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine hydrochloride was added to 75 mL of NMP under a nitrogen atmosphere, and the temperature was adjusted to 20 to 30° C. 3.17 g of NaHCO 3  was added, and the resulting solution was stirred for 10 minutes. 4.35 g of 3-((2-amino-3-chloropyridin-4-yl)thio)-6-chloropyrazin-2-amine and 5.2 g of K 2 CO 3  were added, and the reaction solution was bubbled with nitrogen for 30 minutes. The reaction solution was warmed up to 85° C., and stirred for 8 hours. The reaction solution was cooled to room temperature, and 120 mL of DCM and 150 mL of pure water were added. The solution was stirred and separated into two phases, and the aqueous phase was extracted with 120 mL of DCM once. The organic phases were combined, and washed with pure water (180 mL) five times to remove NMP and 15% aqueous sodium chloride solution (180 mL) once. 0.75 g of activated carbon was added to the DCM phase, which was then stirred for 1 hour and filtered, and the filter cake was rinsed with 30 mL of THF. 1 g of mercapto silica gel was added to the filtrate, which was then stirred for 3 hours and filtered, and the filter cake was rinsed with 30 ml of THF. The filtrate was concentrated to dryness to obtain 9 g of yellow oily solid. 30 mL of EA/MTBE (1/1) was added, and the solution was heated to 50° C. and pulped for 0.5 hour. The solution was naturally cooled to 20° C., stirred for 4 hours, and filtered. The filter cake was rinsed with MTBE, and dried under vacuum to obtain 3.6 g of light yellow solid. According to detection and analysis, it had the XRPD data shown in Table 6, the XRPD pattern shown in  FIG.  12   , the DSC spectrum shown in  FIG.  13   , and the TGA spectrum shown in  FIG.  14   . 
     2.2 Preparation of Crystal Form B of (S)-8-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     About 20 mg of crystal form A of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was weighed, and dissolved in 0.6 mL of 2-methyl-tetrahydrofuran at 70° C. under heating to be a clear solution. The solution was quickly placed into 2° C. condition, and stirred for 12 hours to precipitate a solid. The mixture was centrifuged at high speed, the supernatant was removed, and the solid was vacuum dried at 40° C. to obtain crystal form B of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. According to detection and analysis, it had the XRPD data shown in Table 7, the XRPD pattern shown in  FIG.  15   , and the DSC spectrum shown in  FIG.  16   . 
     2.3 Preparation of Crystal Form C of (S)-8-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine 
     About 20 mg of crystal form A of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was weighed in a 2 mL glass flask, and dissolved in 200 μL of NMP at room temperature under stirring to be a clear solution. 0.6 mL of isopropyl ether was added, and the mixture was stirred at room temperature for 12 hours to precipitate a solid. The mixture was centrifuged at high speed, the supernatant was removed, and the solid was dried under vacuum at 40° C. to obtain crystal form C of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine. According to detection and analysis, it had the XRPD data shown in Table 8, the XRPD pattern shown in  FIG.  17   , and the DSC spectrum shown in  FIG.  18   . 
     3. Screening of Various Crystal Forms of (S)-8-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine and Determination of Stable Crystal Forms 
     3.1 Experimental Objective: 
     The objective is to find stable crystal forms of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine by polymorphic screening. 
     3.2 Experimental Method and Results 
     (1) Transformation Test in Slurry 
     About 20 mg of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was weighed in a 2 mL glass flask, followed by the addition of 10 to 200 μL of relative organic solvents respectively, and the samples were uniformly shaken at 50° C. for 4 days. If the sample was a clear solution, the solution would be placed into room temperature and stirred. The suspension was centrifuged at high speed, the supernatant was removed, and the solid was dried under vacuum at 40° C. The XRPD, DSC and TGA of the resulting solids were determined and compared. Experimental results are shown in Table 3.1. 
     
       
         
           
               
             
               
                 TABLE 3.1 
               
             
            
               
                   
               
               
                 Results of transformation test in slurry 
               
            
           
           
               
               
               
            
               
                   
                 Solvent 
                 Crystal form 
               
               
                   
                   
               
               
                   
                 MeOH 
                 A 
               
               
                   
                 EtOH 
                 A 
               
               
                   
                 ACN 
                 A 
               
               
                   
                 EA 
                 A 
               
               
                   
                 IPA 
                 A 
               
               
                   
                 DCM 
                 A 
               
               
                   
                 H 2 O 
                 A 
               
               
                   
                 Toluene 
                 A 
               
               
                   
                 IPAC 
                 A 
               
               
                   
                   
               
            
           
         
       
     
     The above results show that crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine didn&#39;t undergo crystal form transformation during pulping in the above solvents, crystal form A is thus stable. 
     (2) Cooling Method and Volatilization Method Test 
     A certain amount of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was weighed in a 2 mL glass flask, and dissolved in some good solvents under heating respectively to be a clear solution. The clear solution was cooled, or an anti-solvent was added to the clear solution, and stirred to see if there would be solid precipitation. If there was solid, the solution would be centrifuged at high speed, the supernatant would be removed, and the solid would be vacuum dried at 40° C. If there was no solid, the solution would be kept open at room temperature to evaporate the solvent. The XRPD, DSC and TGA of the resulting solids were determined and compared. Experimental results are shown in Table 3.2. 
     
       
         
           
               
             
               
                 TABLE 3.2 
               
             
            
               
                   
               
               
                 Cooling method and volatilization method test 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Volatilization  
               
               
                   
                 Solvent 
                 Cooling 
                 Anti-solvent 
                 results 
               
               
                   
                   
               
               
                   
                 2-Me-THF 
                 Crystal form 
                 — 
                 — 
               
               
                   
                   
                 B 
                   
                   
               
               
                   
                 MeOH 
                 No solid 
                 — 
                 Crystal form A 
               
               
                   
                   
                 precipitation 
                   
                   
               
               
                   
                 DCM 
                 No solid 
                 — 
                 Crystal form A 
               
               
                   
                   
                 precipitation 
                   
                   
               
               
                   
                 THF 
                 No solid 
                 Still no solid  
                 Crystal form A 
               
               
                   
                   
                 precipitation 
                 precipitation 
                   
               
               
                   
                   
                   
                 after adding  
                   
               
               
                   
                   
                   
                 isopropyl ether 
                   
               
               
                   
                 88%  
                 No solid 
                 — 
                 Crystal form A 
               
               
                   
                 Acetone 
                 precipitation 
               
               
                   
                   
               
            
           
         
       
     
     The above results show that crystal form B of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine can be obtained by the rapid cooling method using 2-Me-THF as the solvent. No solid was precipitated by the rapid cooling method using MeOH, DCM, THE or 88% Acetone as the solvent, and crystal form A was still obtained after volatilization. 
     (3) Solventing-out Crystallization Method Test 
     A certain amount of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was weighed in a 2 mL glass flask, and dissolved in some solvents (as good solvents) respectively to be a clear solution. Some solvents (as anti-solvents) were added dropwise to the solution respectively, which was stirred to see if there would be solid precipitation. If there was solid, the solution would be centrifuged at high speed, the supernatant would be removed, and the solid would be dried under vacuum at 40° C. The XRPD, DSC and TGA of the resulting solids were determined and compared. 
     Experimental results are shown in Table 3.3. 
     
       
         
           
               
             
               
                 TABLE 3.3 
               
             
            
               
                   
               
               
                 Solventing-out crystallization method test 
               
            
           
           
               
               
               
            
               
                 Good solvent 
                 Anti-solvent 
                 Experimental results 
               
               
                   
               
               
                 DMF 
                 H 2 O 
                 A 
               
               
                 DMSO 
                   
                 A 
               
               
                 THF 
                 n-Heptane 
                 A 
               
               
                 NMP 
                 Isopropyl ether 
                 C 
               
               
                   
               
            
           
         
       
     
     The above results show that crystal forms A an C of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine were obtained by the solventing-out crystallization screening. 
     3.3 Experimental Conclusion: 
     Three crystal forms of (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, i.e., crystal forms A, B and C, were obtained by pulping, changing crystallization solvent and crystallization method. By comparing the DSC spectrums of different crystal forms of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, it can be seen that crystal form A has better thermodynamic stability, and is anhydrous. 
     4. Solid Stability Experiment 
     4.1 Experimental Objective: 
     The physicochemical stability of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine under the conditions of high temperature (60° C.), room temperature/high humidity (92.5% RH) and high temperature/high humidity (50° C. and 75% RH) was investigated, so as to provide a basis for the storage of the compound. 
     4.2 Experimental Scheme: 
     About 2 mg of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was investigated under the conditions of high temperature (60° C.), room temperature/high humidity (92.5% RH) and high temperature/high humidity (50° C. and 75% RH) for 5, 10, 20 and 30 days. The content was determined by HPLC using the external standard method. The related substance change was calculated by chromatographic peak area normalization method. 
     4.3 Experimental Results: The stability results are shown in Table 3.4. 
     
       
         
           
               
             
               
                 TABLE 3.4 
               
             
            
               
                   
               
               
                 Solid stability results 
               
            
           
           
               
               
            
               
                   
                 Stability results of crystal form A 
               
               
                   
                 (maximum single impurity increase %) 
               
               
                   
                 Days 
               
            
           
           
               
               
               
               
               
            
               
                 Condition 
                 5 days 
                 10 days 
                 20 days 
                 30 days 
               
               
                   
               
               
                 High temperature (60° C.) 
                 &lt;0.05 
                 &lt;0.05 
                 &lt;0.10 
                 &lt;0.10 
               
               
                 Room temperature/high  
                 &lt;0.05 
                 &lt;0.05 
                 &lt;0.14 
                 &lt;0.12 
               
               
                 humidity (92.5% RH) 
                   
                   
                   
                   
               
               
                 High temperature/ 
                 &lt;0.05 
                 &lt;0.05 
                 &lt;0.13 
                 &lt;0.10 
               
               
                 high humidity 
                   
                   
                   
                   
               
               
                 (50° C. and 75% RH) 
               
               
                   
               
            
           
         
       
     
     4.4 Experimental Conclusion: 
     The above data show that crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine is stable under the conditions of high temperature (60° C.), room temperature/high humidity (92.5% RH) and high temperature/high humidity (50° C. and 75% RH), and the impurity increase is less than 0.15%. 
     5. Hygroscopicity Experiment 
     5.1 Experimental Objective 
     The hygroscopicity of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine under different relative humidity conditions was investigated, so as to provide a basis for the crystal form screening and storage of the compound. 
     5.2 Experimental scheme: 
     Crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was placed in saturated water vapor with different relative humidity to achieve dynamic equilibrium between the compound and water vapor, and the percentage of hygroscopic weight gain of the compound after the equilibrium was calculated. 
     5.3 Experimental Results: 
     Crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine had a hygroscopic weight gain of about 0.544% under RH 80% condition, and had slight hygroscopicity. After one cycle of humidification and dehumidification under 0 to 95% relative humidity condition, the XRPD pattern of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine didn&#39;t change, i.e., the crystal form didn&#39;t transform. 
     6. Solubility Experiment in Different Media 
     6.1 Experimental Objective 
     The solubility of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine in media such as buffers with pH 1 to 8 (hydrochloric acid buffers with pH=1 and 2, phthalic acid buffers with pH=3 and 4, and phosphate buffers with pH=5, 6, 7 and 8), water, Fasted State Simulated Gastric Fluid (FaSGF), Fasted State Simulated Intestinal Fluid (FaSSIF) and Fed State Simulated Intestinal Fluid (FeSSIF) was compared, so as to provide a basis for evaluating the druggability. 
     6.2 Experimental Scheme: 
     About 2 mg of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was suspended in different media for 2 hours, respectively. Thermodynamic solubility of the compound at 37° C. was determined by HPLC using the external standard method. 
     6.3 Experimental results are shown in Table 3.5. 
     
       
         
           
               
             
               
                 TABLE 3.5 
               
             
            
               
                   
               
               
                 Solubility experiment in different media 
               
            
           
           
               
               
               
            
               
                   
                 Media 
                 Solubility of crystal form A (mg/mL) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 pH 1.0 
                 &gt;1.648 
               
               
                   
                 pH 2.0 
                 &gt;1.485 
               
               
                   
                 pH 3.0 
                 0.700 
               
               
                   
                 pH 4.0 
                 0.446 
               
               
                   
                 pH 5.0 
                 0.223 
               
               
                   
                 pH 6.0 
                 0.401 
               
               
                   
                 pH 7.0 
                 0.062 
               
               
                   
                 pH 8.0 
                 0.017 
               
               
                   
                 H 2 O 
                 0.005 
               
               
                   
                 FaSSGF 
                 &gt;1.707 
               
               
                   
                 FaSSIF 
                 0.088 
               
               
                   
                 FeSSIF 
                 0.432 
               
               
                   
                   
               
            
           
         
       
     
     6.4 Experimental Conclusion: 
     By comparing the solubility of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine in different media, it can be seen that crystal form A has better solubility under acidic conditions. 
     7. Crystal Transformation Investigation Experiment 
     7.1 Experimental Objective: 
     The objective is to investigate whether crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine will undergo crystal transformation by pressure crystal transformation and grinding crystal transformation experiments. 
     7.2 Experimental Scheme: 
     A certain amount of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was weighed, and compressed into tablets with a tablet machine. The tablets were mashed to powder and characterized by XRPD. 
     A certain amount of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was weighed, vigorously ground with a mortar for 15 minutes, and characterized by XRPD. 
     7.3 Experimental Conclusion: 
     By comparing the XRPD patterns, it can be seen that crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine didn&#39;t undergo crystal transformation after tabletting and grinding, crystal form A is thus stable. 
     8. Pharmacokinetics Study in Rats 
     8.1 Experimental Objective: 
     The pharmacokinetic behavior of crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine after single oral administration in rat body (plasma) was studied, and the bioavailability for oral administration was calculated. 
     8.2 Experimental Instruments and Reagents: 
     8.2.1 Instruments: 
     
       
         
           
               
               
               
             
               
                   
               
               
                 Instrument 
                 Manufacturer 
                 Model 
               
               
                   
               
             
            
               
                 Analytical 
                 Sartorius Scientific Instruments 
                 SQP 
               
               
                 Balances 
                 (Beijing) Co., Ltd. 
                   
               
               
                 Centrifuge 
                 EPPENDORF 
                 Centrifuge 5415R 
               
               
                 Ultrasonic 
                 Kun Shan Ultrasonic 
                 KQ-100B 
               
               
                 cleaner 
                 Instruments Co., Ltd. 
                   
               
               
                 Pipette 
                 Eppendorf Lab Technologies 
                 YE5K655563(1000 uL) 
               
               
                   
                 (Shanghai) Co., Ltd. 
                 YE5A592012(200 uL) 
               
               
                   
                   
                 YE4A298094(100 uL) 
               
               
                   
                   
                 YE4A405553(10 uL) 
               
               
                 Triple 
                 Thermo Fisher Scientific Inc. 
                 Thermo TSQ Ultra 
               
               
                 quadrupole mass 
                   
                   
               
               
                 spectrometry 
                   
                   
               
               
                 High 
                 Agilent Technologies, Inc. 
                 Agilent 1100 
               
               
                 performance 
                   
                   
               
               
                 liquid 
                   
                   
               
               
                 chromatography 
                   
                   
               
               
                 Ultrasonic cell 
                 Nanjing Shunma Instrument 
                 SM-650D 
               
               
                 disruptor 
                 Equipment Co., Ltd. 
                   
               
               
                 4° C. refrigerator 
                 Hefei Meiling Co., Ltd. 
                 SC-316 
               
               
                 −20° C. 
                 Zhongke Meiling Cryogenics 
                 DW-YL450 
               
               
                 refrigerator 
                 Co., Ltd. 
               
               
                   
               
            
           
         
       
     
     8.2.2 Reagents: 
     
       
         
           
               
               
               
               
             
               
                   
               
               
                 Reagent 
                 Supplier 
                 Batch number 
                 Note 
               
               
                   
               
             
            
               
                 DMSO 
                 Vetech 
                 WXBD0293V 
                   
               
               
                 Acetonitrile 
                 Sigma-Aldrich 
                 WXBD0232V 
                   
               
               
                 Methyl Alcohol 
                 Sigma-Aldrich 
                 WXBC6573V 
                   
               
               
                 Formic Acid 
                 Fisher Scientific 
                 193497 
                   
               
               
                 Dexamethasone 
                 Solarbio 
                 822A0523 
                 Internal 
               
               
                   
                   
                   
                 Standard 
               
               
                 HP-β-CD 
                 Shandong Binzhou Zhiyuan 
                 20190517 
                   
               
               
                   
                 Biotechnology Co., Ltd. 
                   
                   
               
               
                 HPMC K4M 
                 Damas-beta 
                 P1575251 
               
               
                   
               
            
           
         
       
     
     8.3 Test Animals: 
     
       
         
           
               
               
               
             
               
                   
               
               
                 Animal species 
                 Supplier 
                 Certificate No. 
               
               
                   
               
             
            
               
                 SD rats (7 weeks old, body 
                 JOINN (Suzhou)  
                 202009863 
               
               
                 weight 180 to 200 g) 
                 Laboratories Co., Ltd. 
               
               
                   
               
            
           
         
       
     
     8.4 Test Compound: 
     Crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine, self-prepared. 
     8.5 Experimental Scheme: 
     Crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine was suspended evenly in an aqueous solution containing 0.5% HPMC K4M, and then intragastrically administered to rats in triplicate at a dose of 10 mg/kg. 
     8.6 Experimental Results: 
     
       
         
           
               
             
               
                 TABLE 3.6 
               
             
            
               
                   
               
               
                 Results of the pharmacokinetics experiment in rats 
               
            
           
           
               
               
            
               
                   
                 Pharmacokinetics experiment (10 mg/kg) 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                 Area 
                   
                   
                 Average 
                 Bioavail- 
               
               
                   
                 Peak 
                 under curve 
                 plasma 
                 Half 
                 residence 
                 ability 
               
               
                 Test 
                 time 
                 AUC 0-t (ng/ 
                 concentration 
                 life 
                 time 
                 Bio avialibility 
               
               
                 compound 
                 t max (h) 
                 mL*h) 
                 C max (ng/mL) 
                 t 1/2 (h) 
                 MRT 0-∞ (h) 
                 (%) 
               
               
                   
               
               
                 Crystal 
                 1.00 
                 1436.77 
                 359.90 
                 6.99 
                 5.17 
                 76.20 
               
               
                 form A 
               
               
                   
               
            
           
         
       
     
     8.7 Experimental Conclusion: 
     It can be seen from the results of the pharmacokinetics experiment in rats shown in the table that crystal form A of the compound (S)-8-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-2-cyclopropyl-8-azaspiro[4.5]dec-2-en-1-amine of the present invention showed good metabolic property at the dose of 10 mg/kg. 
     IV. Study on the Crystal Forms of (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     Scale-up production of the amorphous form of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine (Example 53) 
     (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)pyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine and THE were added to a reactor, then N-fluorobisbenzenesulfonamide (NFSI) was added, and the reaction was carried out at room temperature for 2 hours. 2 L of 0.5 mol/L hydrochloric acid solution was added, and the reaction solution was extracted with 2 L of EA under stirring and separated into two phases. 3 L of 0.25 mol/L hydrochloric acid solution was added to the organic phase, which was then extracted with 11 L of EA under stirring and separated into two phases. The aqueous phases were combined, and the organic phase was discarded. The aqueous phase was extracted with 1.5 L*2 of EA to remove the impurities, followed by adding 2.65 L of THE and stirring. Solid sodium carbonate was added to adjust pH=9 to 10, and the solution was separated into two phases. The aqueous phase was extracted with 1.5 L of 2-methyltetrahydrofuran. The organic phases were combined, dried, filtered and concentrated to obtain 73.4 g of a tan solid. The solid was dissolved in tetrahydrofuran to be a clear solution, and the resulting product was washed with EA:PE=20%, 60%, EA containing 0.005% triethylamine, and methanol (containing 5% triethylamine):DCM=5% successively. The buffer was concentrated under reduced pressure to obtain 41.1 g of a yellow solid for later use. 
     1.1 Experimental Instruments 
     1.1.1 Some Parameters of Physical and Chemical Testing Instruments 
     
       
         
           
               
               
               
             
               
                   
               
             
            
               
                 XRPD 
                 Instrument model 
                 BRUKER D8 ADVANCE 
               
               
                   
                 Diffraction ray 
                 CuK (40 kV, 25 mA) 
               
               
                   
                 Scan rate 
                 0.02°/S (2θ value) 
               
               
                   
                 Scan range 
                 4° to 40° (2θ value) 
               
               
                 DSC 
                 Instrument model 
                 NETZSCH DSC 214 polyma 
               
               
                   
                 Purge gas 
                 Nitrogen 
               
               
                   
                 Purge rate 
                 40 mL/min 
               
               
                   
                 Heating rate 
                 10° C./min 
               
               
                   
                 Temperature range 
                 25 to 350° C. 
               
               
                   
                 Plate type 
                 Aluminum plate 
               
               
                 TGA 
                 Instrument model 
                 NETZSCH TG 209 Tarsus 
               
               
                   
                 Purge gas 
                 Nitrogen 
               
               
                   
                 Purge rate 
                 40 mL/min 
               
               
                   
                 Heating rate 
                 10° C./min 
               
               
                   
                 Temperature range 
                 Room temperature~400° C. 
               
               
                   
                 Plate type 
                 Al 2 O 3   
               
               
                   
               
            
           
         
       
     
     1.2 Instruments and Liquid Chromatography Conditions 
     1.2.1 Instruments and Devices 
     
       
         
           
               
               
               
             
               
                   
                   
               
               
                   
                 Instrument name 
                 Model 
               
               
                   
                   
               
             
            
               
                   
                 Analytical Balances 
                 Sartorius BSA224S-CW 
               
               
                   
                 Water purifier 
                 Milli-Q Plus, Millipore 
               
               
                   
                 High performance liquid 
                 Agilent1260 
               
               
                   
                 chromatography 
                   
               
               
                   
                 Pump 
                 Agilent G1311B 
               
               
                   
                 Injector 
                 G1329B 
               
               
                   
                 Column oven 
                 G1316A 
               
               
                   
                 Detector 
                 G1315D 
               
               
                   
                   
               
            
           
         
       
     
     1.2.2 Chromatographic Conditions 
     Column: ZORBAX (SB-C8, 3.5 μm, 4.6*75 mm) 
     Flow rate: 1 mL/min 
     Column temperature: 40° C. 
     Detection wavelength: 275 nm 
     Injection volume: 5.0 μL 
     Running time: 13 min 
     Diluent: ACN-water (v/v, 1:1) 
     Mobile phase: A: water (0.05% trifluoroacetic acid); B: acetonitrile (0.05% trifluoroacetic acid) 
     
       
         
           
               
               
               
             
               
                   
               
               
                 T(min) 
                 A (%) 
                 B (%) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 0.00 
                 90 
                 10 
               
               
                 3.00 
                 70 
                 30 
               
               
                 10.00 
                 40 
                 60 
               
               
                 10.10 
                 90 
                 10 
               
               
                 13.00 
                 90 
                 10 
               
               
                   
               
            
           
         
       
     
     1. Preparation of Various Crystal Forms of (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine 
     1.1 Preparation of Crystal Form A of (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-amine 
     10 mg of the amorphous form of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine was weighed in a 2 mL glass flask, followed by the addition of 100 μL of 3-pentanone, and the mixture was stirred at room temperature for 24 hours. The system was clear, and then a solid was precipitated during stirring. The mixture was centrifuged to remove the supernatant, and the remaining solid was dried in a vacuum drying oven at 40° C. to constant weight to obtain crystal form A of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. According to detection and analysis, it had the XRPD data shown in Table 1, the DSC spectrum shown in  FIG.  1   , and the TGA spectrum shown in  FIG.  2   . 
     1.2 Preparation of Crystal Form B of (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-amine 
     20 mg of the amorphous form of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine was weighed in a 2 mL glass flask, and dissolved in 200 μL of acetonitrile at room temperature to be a clear solution. 400 μL of H 2 O was slowly added to the system at room temperature. H 2 O was added under stirring, and a solid was precipitated soon. The mixture was stirred at room temperature for 2 hours, centrifuged to remove the supernatant, and the remaining solid was dried in a vacuum drying oven at 40° C. to constant weight to obtain crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. According to detection and analysis, it had the XRPD data shown in Table 2, the DSC spectrum shown in  FIG.  4   , and the TGA spectrum shown in  FIG.  5   . 
     1.3 Preparation of Crystal Form C of (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-amine 
     20 mg of the amorphous form of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine was weighed in a 2 mL glass flask, and dissolved in 50 L of dichloromethane at room temperature to be a clear solution. The solution was stirred at room temperature for 24 hours, and a solid was precipitated. The mixture was rapidly centrifuged to remove the supernatant, and the remaining solid was dried in a vacuum drying oven at 40° C. to constant weight to obtain crystal form C of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. According to detection and analysis, it had the XRPD data shown in Table 3, the DSC spectrum shown in  FIG.  6   , and the TGA spectrum shown in  FIG.  7   . 
     1.4 Preparation of Crystal Form D of (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-amine 
     20 mg of the amorphous form of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine was weighed in a 2 mL glass flask, and dissolved in 50 L of 1,4-dioxane at room temperature to be a clear solution. The solution was stirred at room temperature for 24 hours, and a solid was precipitated. The mixture was rapidly centrifuged to remove the supernatant, and the remaining solid was dried in a vacuum drying oven at 40° C. to constant weight to obtain crystal form D of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. According to detection and analysis, it had the XRPD data shown in Table 4, the DSC spectrum shown in  FIG.  8   , and the TGA spectrum shown in  FIG.  9   . 
     1.5 Preparation of Crystal Form E of (S)-1′-(6-Amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydro spiro[indene-2,4′-piperidin]-1-amine 
     20 mg of the amorphous form of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine was weighed in a 2 mL glass flask, and dissolved in 100 μL of DMF at room temperature to be a clear solution. 400 μL of H 2 O was slowly added to the system at room temperature. H 2 O was added under stirring, and a solid was precipitated soon. The mixture was stirred at room temperature for 2 hours, centrifuged to remove the supernatant, and the remaining solid was dried in a vacuum drying oven at 40° C. to constant weight to obtain crystal form E of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine. According to detection and analysis, it had the XRPD data shown in Table 5, the DSC spectrum shown in  FIG.  10   , and the TGA spectrum shown in  FIG.  11   . 
     2. Solid Stability Experiment 
     2.1 Experimental Objective: 
     The physicochemical stability of the amorphous form, crystal form A and crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine under light condition was investigated, so as to provide a basis for the crystal form screening and storage of the compound. 
     2.2 Experimental Scheme: 
     About 2 mg of the amorphous form, crystal form A and crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine were placed in a light box (5000 lx±500 lx) respectively to investigate for 5 and 10 days. The content was determined by HPLC using the external standard method. The related substance change was calculated by chromatographic peak area normalization method. 
     2.3 Experimental Results: 
     The physicochemical stability results of different crystal forms of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine are shown in Table 4.1. 
     
       
         
           
               
             
               
                 TABLE 4.1 
               
             
            
               
                   
               
               
                 Physicochemical stability results of different crystal forms 
               
            
           
           
               
               
            
               
                   
                 Light 5000 ± 500 lux (sealed) 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 Time point 
                   
                 Total 
                   
                 Total 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                 Total 
                   
                 Total 
                 impurities 
                   
                 Total 
                 impurities 
               
               
                 Condition 
                 Day 0 
                 impurities 
                 Day 5 
                 impurities 
                 gain 
                 Day 10 
                 impurities 
                 gain 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                 Amorphous 
                 98.09% 
                 1.91% 
                 — 
                 — 
                 — 
                 78.75% 
                 21.25% 
                 19.34% 
               
               
                 form 
               
               
                 Crystal 
                 99.14% 
                 0.86% 
                 98.23% 
                 1.77% 
                 0.91% 
                 97.29% 
                 2.71% 
                 1.85% 
               
               
                 form A 
               
               
                 Crystal 
                 98.34% 
                 1.66% 
                 97.28% 
                 2.72% 
                 1.06% 
                 96.46% 
                 3.54% 
                 1.88% 
               
               
                 form B 
               
               
                   
               
            
           
         
       
     
     The above data show that the 10-day total impurities gain of the amorphous form of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine under light condition is 19.34%, whereas the 10-day total impurities gain of crystal form A and crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine under light condition is less than 2%. 
     2.4 Experimental Conclusion: 
     The stability under light condition can be improved by preparing (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine into a crystal form. 
     3. Hygroscopicity Experiment 
     3.1 Experimental Objective 
     The hygroscopicity of crystal form B of the compound (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine under different relative humidity conditions was investigated, so as to provide a basis for the crystal form screening and storage of the compound. 
     3.2 Experimental Scheme: 
     Crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine was placed in saturated water vapor with different relative humidity to achieve dynamic equilibrium between the compound and water vapor, and the percentage of hygroscopic weight gain of the compound after the equilibrium was calculated. 
     3.3 Experimental Results: 
     Crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine had a hygroscopic weight gain of about 1.639% under RH 80% condition, and had slight hygroscopicity. After one cycle of humidification and dehumidification under 0 to 95% relative humidity condition, the XRPD pattern of crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine didn&#39;t change, i.e., the crystal form didn&#39;t transform. 
     4. Solubility Experiment in Different Media 
     4.1 Experimental Objective 
     The solubility of crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine in media such as water, Simulated Gastric Fluid (SGF), Fasted State Simulated Intestinal Fluid (FaSSIF) and Fed State Simulated Intestinal Fluid (FeSSIF) was compared, so as to provide a basis for evaluating the druggability. 
     4.2 Experimental scheme: 
     About 2 mg of crystal form B of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine was suspended in different media for 24 hours, respectively. Thermodynamic solubility of the compound at 37° C. was determined by HPLC using the external standard method. 
     4.3 Experimental results are shown in Table 4.2. 
     
       
         
           
               
             
               
                 TABLE 4.2 
               
             
            
               
                   
               
               
                 Solubility experiment in different media 
               
            
           
           
               
               
               
            
               
                   
                 Sample name 
                 Solubility of crystal form B (mg/mL) 
               
               
                   
                   
               
               
                   
                 Fa 
                 0.170 
               
               
                   
                 Fe 
                 0.064 
               
               
                   
                 SGF 
                 2.050 
               
               
                   
                 water 
                 0.001 
               
               
                   
                   
               
            
           
         
       
     
     5. Polymorphic Screening Experiment and Determination of Stable Crystal Form 
     5.1 Experimental Objective: 
     The objective is to find stable crystal forms of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine by polymorphic screening. 
     5.2 Experimental scheme: 
     Organic solvents and water with a certain solubility was chosen to suspend (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine in the solvent system, and the mixture was stirred and slurried at room temperature for one week, and then centrifuged. The supernatant was discarded, and the solid was dried under vacuum (−0.1 Mpa) at 40° C. overnight. The XRPD, DSC and TGA of the solid was determined and compared. 
     5.3 Experimental Results: 
     Five crystal forms of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, i.e., crystal forms A, B, C, D and E, were obtained by pulping, changing crystallization solvent and crystallization method. By comparing the DSC spectrums of different crystal forms of (S)-1′-(6-amino-5-((2-amino-3-chloropyridin-4-yl)thio)-3-fluoropyrazin-2-yl)-1,3-dihydrospiro[indene-2,4′-piperidin]-1-amine, it can be seen that crystal form B has the best thermodynamic stability among the five crystal forms.