Patent Publication Number: US-PP33183-P2

Title: Hemp plant named ‘C2B’

Description:
Latin name of the genus and species of the plant claimed:  Cannabis  spp. 
     Variety denomination: ‘C2B’. 
     BACKGROUND OF THE INVENTION 
       Cannabis  is the genus of a variety of species— Cannabis sativa, Cannabis ruderalis,  and  Cannabis indica —which is often used as an umbrella term to refer to them all. This misclassification of the different species has made it difficult to properly distinguish between and understand the best ways to utilize the different varieties of these plants. 
     According to the 2018 Farm Bill, Hemp is a variety of  Cannabis sativa  that is distinguished by its low tetrahydrocannabinol (THC) levels of less than 0.3%. THC is the only currently known psychoactive compound found in  Cannabis , however there are many additional cannabinoid compounds that can be utilized in a variety of ways. The exact concentration results based on lab testing of dried flowers will vary depending on growing conditions of the plant, and sampling, preparation, and testing methods used. THC production, for example, is a natural defense mechanism for the plant, meaning in high stress or threatening environments the specific plant will produce higher levels of THC. Because of this inconsistency, many state labs are allowing slightly higher levels of THC in the tests as anything under 1% THC has not been proven to have psychoactive effects. 
     For the purpose of this study on ‘C2B’, the cannabinoid percentages are recorded based on a plant grown in a high-stress environment to determine the maximum concentration of THC that will be produced by this specific strain. The results conclude that the ‘C2B’ is a type-III hemp cultivar meaning it does not possess the allele to ever make more than 0.5% THC (and thus is not suitable for marijuana use). Additionally, ‘C2B’ is a new, unique variety because of its composition of other cannabinoids, terpenes, and flavonoids. Unlike many other  Cannabis  varieties, ‘C2B’ has a 25:9:1 cannabidiol:cannabichromene:tetrahydrocannabinol (CBD:CBC:THC) ratio which has not been identified thus far in other strains. Therefore, ‘C2B’ can be used to extract CBD and CBC for medicinal applications. 
     With the recent legalization of hemp in 2018, the studies on medicinal effects of CBD are only at the beginning. CBD has been proven to alleviate nausea, chronic pain, and spasticity due to MS, to reduce blood pressure, to work as an anticonvulsant, antipsychotic, and anti-inflammatory and so much more. CBC, on the other hand, has only been isolated in such small concentrations that extensive research is required to continue understanding the possibilities for this compound. So far, CBC has been shown to act as an antidepressant and anti-inflammatory, is used to treat acne and hypermotility, and is rumored to kill cancer cells. 
     ‘C2B’ was developed in a selective breeding program by performing controlled fertilization of known, high-performing hemp varieties. It was created by breeding both  Cannabis sativa  and  Cannabis indica  species together to create this unique hybrid. The purpose of the research was to determine what methods would allow for low THC levels in stressful, less-than-ideal environmental conditions (e.g., low-pH soil, high-salinity soil, dry environments, short growing seasons, etc.). The research also was striving to isolate varieties of plants with high concentrations of cannabinoids that have not been isolated in mass. ‘C2B’ was the best-performing individual selected from 200 seed phenotypes with CBC levels nearly four to eight times the industry average. Most  Cannabis  varieties have no detectable level of CBC, and none of the other plants of the 200 seed phenotypes exhibited more than 50% of the ‘C2B’ CBC levels. 
     BRIEF SUMMARY OF THE INVENTION 
     This invention relates to a new and distinctive hemp cultivar designated as ‘C2B’, wherein ‘C2B’ comprises a cannabidiol:cannabichromene:tetrahydrocannabinol ratio of 25:9:1 which is 4-8 times the average amount of cannabichromene found in other  Cannabis  varieties. 
     As used herein, the term “cultivar” is used interchangeably with the terms “variety,” strain,” and/or “clone.” 
     Progenies have been reproduced asexually via apical stem cuttings from vegetative plants. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The accompanying photographs illustrate the new hemp variety: 
         FIG. 1  shows a perspective view of a CC mother on the left next to a ‘C2B’ on the right, both grown from clones and planted in soil at the same time, at 23 days from the time the clones were planted in soil; 
         FIG. 2  shows top views of apical buds of a CC mother on the left and of a ‘C2B’ on the right, at 21 days after the flowering cycle was introduced; 
         FIG. 3  shows a perspective view of a defoliated ‘C2B’ plant on day 26 of the flowering cycle (due to defoliation, branching is not natural); 
         FIG. 4  shows a perspective view of a ‘C2B’ plant on day 25 after the rockwool clone was planted in soil, grown in 24-hour light with no defoliation or manipulation other than initially being planted in a 3-inch pot then being transplanted to a 3-gallon pot; 
         FIG. 5  shows a perspective view of ‘C2B’ taken two weeks prior to harvest; 
         FIG. 6  shows a close-up perspective view of ‘C2B’ stem, illustrating stem shape; and 
         FIG. 7  shows a close-up perspective view of CC mother stem, illustrating stem shape. 
     
    
    
     DETAILED BOTANICAL DESCRIPTION 
     ‘C2B’ has been examined in manipulated conditions grown indoors in Black Point, Calif. The variety has not been grown in all possible conditions, thus different environmental factors alter the appearance or composition of this phenotype. The individual has been reproduced asexually via apical stem cuttings from vegetative plants and remains stable and reproduces true to type in successive generations of asexual reproduction. 
     In the following description, the color determination is in accordance with The Royal Horticultural Society Colour Charts, Fifth Edition, except where general color terms of ordinary dictionary significance are used. 
     
       
         
           
               
             
               
                 TABLE I 
               
               
                   
               
               
                 General 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Characteristics 
                 New Variety 
               
               
                   
               
               
                 Plant Life Form 
                 Herbaceous plant (herb) 
               
               
                 Plant Growth 
                 Upright, dioecious, annual, no hermaphrodite, 
               
               
                 Habit 
                 or monoecious, tendencies without stress 
               
               
                   
                 conditions. 
               
               
                 Plant origin 
                 ‘C2B’ was created in a selective breeding 
               
               
                   
                 program from a cross between the Mother, 
               
               
                   
                 CC, hemp variety and the Father, K, hemp 
               
               
                   
                 variety. 
               
               
                 Plant Propagation 
                 Asexually reproduced via apical stem 
               
               
                   
                 cutting and cloning from mother in 
               
               
                   
                 vegetative state. 
               
               
                 Propagation ease 
                 Easy 
               
               
                 Propagation 
                 80° F., 90% humidity 
               
               
                 Condition 
                   
               
               
                 Height (unit: feet) 
                 Outdoors may reach 15-17 feet at maturity. 
               
               
                   
                 Indoors may reach 3-6 feet at maturity 
               
               
                   
                 depending on growth conditions. 
               
               
                 Width (unit: feet) 
                 Outdoors may reach 10 feet at maturity. 
               
               
                   
                 Indoors may reach 1-2 feet depending on 
               
               
                   
                 growth conditions. This plant is more slender 
               
               
                   
                 than typical  Cannabis  plants having less 
               
               
                   
                 foliage and large internodes. 
               
               
                 Time to Harvest 
                 75 Days from Induction of flowering light cycle 
               
               
                 Resistance to 
                 Exhibits resistance to  Podosphaera   macularis   
               
               
                 Pests or disease 
                 (Powdery Mildew) and  Botrytis cinerea   
               
               
                   
                 (Grey Mold Fungus). Has resistance to  
               
               
                   
                   Phorodon cannabis  (Bhang Aphid),  Myzus   
               
               
                   
                   persicae  (Green Peach Aphid), Aphis fabae 
               
               
                   
                 (Black Bean Aphid),  Tetranychu   surticae  (Two- 
               
               
                   
                 Spotted Spider Mite) and  Panonychus ulmi   
               
               
                   
                 (Red Spider Mite). 
               
               
                 Genetically Modified 
                 No 
               
               
                 Organism? 
               
               
                   
               
               
                   
                 Parental Variety (CC) 
               
               
                 Characteristics 
                 (Female Plant) 
               
               
                   
               
               
                 Plant Life Form 
                 Herbaceous plant (herb) 
               
               
                 Plant Growth Habit 
                 Upright, dioecious, annual, no hermaphrodite 
               
               
                   
                 or monoecious tendencies without stress 
               
               
                   
                 conditions. 
               
               
                 Plant origin 
                 Origin unknown 
               
               
                 Plant Propagation 
                 Asexually reproduced via apical 
               
               
                   
                 stem cutting and cloning from CC 
               
               
                   
                 in vegetative state. 
               
               
                 Propagation ease 
                 Easy 
               
               
                 Propagation 
                 80° F., 90% humidity 
               
               
                 Condition 
                   
               
               
                 Height (unit: feet) 
                 Outdoors may reach 8-10 feet at maturity. 
               
               
                   
                 Indoors may reach 3-4 feet 
               
               
                   
                 depending on growth conditions. 
               
               
                 Width (unit: feet) 
                 Outdoors may reach 6-8 feet. 
               
               
                   
                 Indoors may reach 1-2 feet. 
               
               
                 Time to Harvest 
                 60 Days from Induction of flowering 
               
               
                   
                 light cycle 
               
               
                 Resistance to 
                 More-prone to Podosphaera macularis 
               
               
                 Pests or disease 
                 (Powdery Mildew) 
               
               
                   
                 than ‘C2B’. Has resistance to  Phorodon   
               
               
                   
                   cannabis  (Bhang Aphid),  Myzus persicae   
               
               
                   
                 (Green Peach Aphid),  Aphis   fabae   
               
               
                   
                 (Black Bean Aphid) and  Tetranychus urticae   
               
               
                   
                 (Two-Spotted Spider Mite and Red 
               
               
                   
                 Spider Mite). 
               
               
                 Genetically Modified 
                 No 
               
               
                 Organism? 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE II 
               
               
                   
               
               
                 Leaf/Foliage 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Characteristics 
                 New Variety 
               
               
                   
               
               
                 Leaf Arrangement 
                 Spiral alternate leaf arrangement 
               
               
                   
                 when grown from a cutting. Stem is 
               
               
                   
                 less angled between nodes as 
               
               
                   
                 compared to mother. 
               
               
                 Leaf Shape 
                 Palmately compound with 3-5 leaflets 
               
               
                   
                 in mature growth. 
               
               
                 Leaf Structure 
                 Sharply serrated margins. Skinny 
               
               
                   
                 oval to lanceolate leaflets, sometimes 
               
               
                   
                 appear falcate. Apex and base are 
               
               
                   
                 acuminate, apex longer tapering. 
               
               
                   
                 More narrow leaf 
               
               
                 Leaf Margins 
                 Serrate margins. Outer side of the 
               
               
                   
                 serration is concave to straight. Inner 
               
               
                   
                 side is convex. Tip of each serration 
               
               
                   
                 slightly curls. 
               
               
                 Leaf Hairs 
                 Absent 
               
               
                 Leaf Length with 
                 15-22 cm 
               
               
                 Petiole at 
                   
               
               
                 Maturity 
                   
               
               
                 Petiole Length at 
                 4-8 cm 
               
               
                 Maturity 
                   
               
               
                 Petiole Color 
                 149B to 150A 
               
               
                 (RHS Number) 
                   
               
               
                 Anthocyanin 
                 Green but may show light lavender 
               
               
                 color and 
                   
               
               
                 intensity in 
                   
               
               
                 Petioles 
                   
               
               
                 Stipule length at 
                 1-4 mm 
               
               
                 maturity 
                   
               
               
                 Stipule shape 
                 Very small, lance shape. Broad/rounded 
               
               
                   
                 base with acuminate/long tapering apex. 
               
               
                 Stipule Color 
                 149B to 150A 
               
               
                 (RHS Number) 
                   
               
               
                 Number of 
                 3-5 throughout life cycle 
               
               
                 Leaflets 
                   
               
               
                 Middle Largest 
                 8-15 cm 
               
               
                 (longest leaflet) 
                   
               
               
                 length 
                   
               
               
                 Middle Largest 
                 1.1-2.3 cm 
               
               
                 (longest leaflet) 
                   
               
               
                 width 
                   
               
               
                 Middle Largest 
                 About 7:1 
               
               
                 (longest Leaflet) 
                   
               
               
                 length/width 
                   
               
               
                 Ratio 
                   
               
               
                 Number of teeth 
                 28-32 
               
               
                 of middle leaflet 
                   
               
               
                 Leaf (upper side) 
                 141A and 134A 
               
               
                 color (RHS 
                   
               
               
                 Number) 
                   
               
               
                 Leaf (lower side) 
                 144A-B 
               
               
                 color (RHS 
                   
               
               
                 Number) 
                   
               
               
                 Leaf Glossiness 
                 Not very. More matte, slight gloss reflection but 
               
               
                   
                 not shiny, 
               
               
                 Vein/midrib shape 
                 Midveins branch palmately with the leaflets, 2° 
               
               
                   
                 veins are pinnate and branch towards the 
               
               
                   
                 apex of the serrations, they are relatively straight 
               
               
                   
                 until they near the margins and they may angle 
               
               
                   
                 upwards slightly. 3° veins are palmate slightly 
               
               
                   
                 branching from 2° veins. 3° veins close to 
               
               
                   
                 the margin will branch to the dip between 
               
               
                   
                 serrations. Only 1° and 2° veins are 
               
               
                   
                 visible from the top of the leaf 
               
               
                 Vein/midrib color 
                 149B-C 
               
               
                 (RHS Number) 
                   
               
               
                 Aroma 
                 Flower has an earth aroma with a hint 
               
               
                   
                 of berry wine and pepper. 
               
               
                   
               
               
                   
                 Parental Variety (CC) 
               
               
                 Characteristics 
                 (Female Plant) 
               
               
                   
               
               
                 Leaf Arrangement 
                 Spirally alternate when grown from a cutting. 
               
               
                   
                 Stalk alternates directions back and forth 
               
               
                   
                 between branches. 
               
               
                 Leaf Shape 
                 Palmately compound with 5-7 leaflets throughout 
               
               
                   
                 the life cycle. 
               
               
                 Leaf Structure 
                 Serrated margins. Elliptical leaf with tapering base 
               
               
                   
                 and apex. 
               
               
                   
                 Middle section of leaflets are wide. 
               
               
                 Leaf Margins 
                 Evenly serrated. Point of serration is more 
               
               
                   
                 acuminate/tapering to a point. Inner and outer 
               
               
                   
                 sides of serrations are convex. 
               
               
                 Leaf Hairs 
                 None 
               
               
                 Leaf Length with 
                 13.1-19 cm 
               
               
                 Petiole at 
                   
               
               
                 Maturity 
                   
               
               
                 Petiole Length at 
                 3-5.5 cm 
               
               
                 Maturity 
                   
               
               
                 Petiole Color 
                 150A 
               
               
                 (RHS Number) 
                   
               
               
                 Anthocyanin 
                 Purple to green 
               
               
                 color and 
                   
               
               
                 intensity in 
                   
               
               
                 Petioles 
                   
               
               
                 Stipule length at 
                 1.5-3 mm 
               
               
                 maturity 
                   
               
               
                 Stipule shape 
                 Very small, lance shape. Broad/rounded 
               
               
                   
                 base with acuminate/long tapering apex. 
               
               
                 Stipule Color 
                 150A 
               
               
                 (RHS Number) 
                   
               
               
                 Number of 
                 5-7 throughout life cycle 
               
               
                 Leaflets 
                   
               
               
                 Middle Largest 
                 9.3-13 cm 
               
               
                 (longest leaflet) 
                   
               
               
                 length 
                   
               
               
                 Middle Largest 
                 2-4 cm 
               
               
                 (longest leaflet) 
                   
               
               
                 width 
                   
               
               
                 Middle Largest 
                 About 4:1 
               
               
                 (longest Leaflet) 
                   
               
               
                 length/width 
                   
               
               
                 Ratio 
                   
               
               
                 Number of teeth 
                 23-25 
               
               
                 of middle leaflet 
                   
               
               
                 Leaf (upper side) 
                 N144C 
               
               
                 color (RHS 
                   
               
               
                 Number) 
                   
               
               
                 Leaf (lower side) 
                 149A 
               
               
                 color (RHS 
                   
               
               
                 Number) 
                   
               
               
                 Leaf Glossiness 
                 Bottom is matte, top has slight light 
               
               
                   
                 reflection but not shiny 
               
               
                 Vein/midrib shape 
                 Midvein is palmate with leaflets. 2° 
               
               
                   
                 veins are pinnate and straight. 3° 
               
               
                   
                 veins are also palmate and branch 
               
               
                   
                 from 10 and 2° veins. All three types 
               
               
                   
                 of veins are slightly visible from the 
               
               
                   
                 top. 
               
               
                 Vein/midrib color 
                 149C 
               
               
                 (RHS Number) 
                   
               
               
                 Aroma 
                 Flower has a spicy pepper with a 
               
               
                   
                 sweet floral fragrance. 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE III 
               
             
            
               
                   
               
               
                 Stem 
               
            
           
           
               
               
               
            
               
                   
                   
                 Parental Variety 
               
               
                 Characteristics 
                 New Variety 
                 (CC) (Female Plant) 
               
               
                   
               
               
                 Stem Shape 
                 (See FIG. 6) Round at  
                 (See FIG. 7) At maturity,  
               
               
                   
                 maturity. Immature or new  
                 round and in the bottom/ 
               
               
                   
                 growth has ridges, with a  
                 trunk of the plant, growth  
               
               
                   
                 relatively pentagonal shape. 
                 appears woody. Immature  
               
               
                   
                 Young growth is pubescent,  
                 or new growth has ridges, 
               
               
                   
                 older growth loses the hairs.  
                 approximately pentagonal  
               
               
                   
                 Ridges are slightly furrowed,  
                 in shape. Between nodes,  
               
               
                   
                 more protruding angles  
                 the stem angles slightly  
               
               
                   
                 connected by flat sides.  
                 to give a zig-zag  
               
               
                   
                 Nodes, where branches meet  
                 appearance. 
               
               
                   
                 the stalk, bulge to support  
                   
               
               
                   
                 large flowers. Main stem  
                   
               
               
                   
                 internodes are long compared  
                   
               
               
                   
                 to other  Cannabis  cultivars. 
                   
               
               
                 Stem Diameter  
                 2-10 cm 
                 3-12 cm 
               
               
                 at Base 
                   
                   
               
               
                 Stem Color 
                 144A to 146A 
                 144A to 146A 
               
               
                 Stem Pith Type 
                 Thick to woody 
                 Moderate to thick 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE IV 
               
               
                   
               
               
                 Inflorescence 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Characteristics 
                 New Variety 
               
               
                   
                   
               
               
                   
                 Flowering 
                 Dioecious. Once branching also 
               
               
                   
                 (blooming) habit 
                 occurs at the node, the flower will 
               
               
                   
                   
                 grow &gt;1 cm up from the branch 
               
               
                   
                   
                 before producing a flower. Flowers 
               
               
                   
                   
                 grow tubular with a solid cola. 
               
               
                   
                 Proportion of 
                 Around 50% Very Stable Dioecious 
               
               
                   
                 female plants 
                   
               
               
                   
                 Inflorescence 
                 Above branches at nodes. Slight 
               
               
                   
                 Position 
                 stem grown for bud to be 
               
               
                   
                   
                 above/separate from branch. 
               
               
                   
                 Flower 
                 Overlapping, congested, individual 
               
               
                   
                 arrangement 
                 flowers grow in tubular formation 
               
               
                   
                   
                 and grow separate from others. 
               
               
                   
                   
                 Large internodes keep buds 
               
               
                   
                   
                 separate, even if branching on the 
               
               
                   
                   
                 same node, not stacked. 
               
               
                   
                 Number of 
                 Hundreds to thousands 
               
               
                   
                 Flowers per plant 
                   
               
               
                   
                 Flower shape 
                 Asymmetrical growth. Flower itself 
               
               
                   
                   
                 may have bilateral symmetry. 
               
               
                   
                   
                 Surrounding leaves are sparse and 
               
               
                   
                   
                 do not fully circle the flower as with 
               
               
                   
                   
                 other cannabis strains. The leaves 
               
               
                   
                   
                 are more layered with further 
               
               
                   
                   
                 distance between them so they do 
               
               
                   
                   
                 not appear as clustered around the flower. 
               
               
                   
                 Flower 
                 5-10 mm 
               
               
                   
                 (individual 
                   
               
               
                   
                 pistillate) length 
                   
               
               
                   
                 Flower 
                 2-12 cm 
               
               
                   
                 (compound cyme) 
                   
               
               
                   
                 diameter 
                   
               
               
                   
                 Corolla 
                 No defined corolla 
               
               
                   
                 Corolla Color 
                 N/A 
               
               
                   
                 (RHS number) 
                   
               
               
                   
                 Bract shape 
                 Small, ovate with tapering apex. 
               
               
                   
                   
                 Hidden under the leaves 
               
               
                   
                   
                 surrounding the flower, directly 
               
               
                   
                   
                 below each individual flower. Bracts 
               
               
                   
                   
                 are typically 2-3mm and are covered 
               
               
                   
                   
                 in glandular trichomes. 
               
               
                   
                 Bract color (RHS 
                 149B to 150A 
               
               
                   
                 number) 
                   
               
               
                   
                 Bracteole shape 
                 Same as bract. More within the 
               
               
                   
                 (general 
                 flower cluster. Hard to identify 
               
               
                   
                 description) 
                 without dissection. Roughly 0.25- 
               
               
                   
                   
                 1 mm in size; very small. 
               
               
                   
                 Bracteole color 
                 149B to 150A 
               
               
                   
                 (RHS number) 
                   
               
               
                   
                 Calyx Shape 
                 Triangular conical, about 1-3mm 
               
               
                   
                 (general 
                 and covered in glandular trichomes 
               
               
                   
                 description) 
                   
               
               
                   
                 Calyx color (RHS 
                 143A to 143C 
               
               
                   
                 number) 
                   
               
               
                   
                 Stigma shape 
                 Ovate shape with long tapering apex 
               
               
                   
                   
                 where 2 spindle-like styles protrude. 
               
               
                   
                 Stigma length 
                 3-10 mm 
               
               
                   
                 Stigma color 
                 149C to 149D 
               
               
                   
                 (RHS number) 
                   
               
               
                   
                 Trichome shape 
                 Capitate stalked gland, long stalk 
               
               
                   
                   
                 with smaller bulb on top. In mature 
               
               
                   
                   
                 plants grow on flowers, petioles, 
               
               
                   
                   
                 leaves and veins. 
               
               
                   
                 Cystolithic non- 
                 On leaves farther from bud. Leaves 
               
               
                   
                 glandular 
                 grow trichomes that appear 
               
               
                   
                   
                 glandular when surrounding the 
               
               
                   
                   
                 flowers. 
               
               
                   
                 Trichome color 
                 Immature: 142D 
               
               
                   
                 (RHS number) 
                 Mature (when ready to harvest): 172A 
               
               
                   
                 Terminal Bud 
                 When flowering: More radial 
               
               
                   
                 shape 
                 symmetry from leaves growing 
               
               
                   
                   
                 without obstruction from stem. Buds 
               
               
                   
                   
                 generally have similar appearance 
               
               
                   
                   
                 because they grow protruding from 
               
               
                   
                   
                 their node, rather than hugging the 
               
               
                   
                   
                 stem/branch which allows for more 
               
               
                   
                   
                 equal growth around the 
               
               
                   
                   
                 circumference. 
               
               
                   
                   
                 When vegetative: the bud has 
               
               
                   
                   
                 spindly new growth coming out. 
               
               
                   
                   
                 New leaves are very skinny, almost 
               
               
                   
                   
                 hair-like and do not grow as straight 
               
               
                   
                   
                 as other cannabis plants. 
               
               
                   
                 Terminal Bud 
                 Est. N144D and 150C 
               
               
                   
                 color (RHS 
                   
               
               
                   
                 number) 
                   
               
               
                   
                 Pedicle 
                 Present in varying lengths 
               
               
                   
                   
                 depending on maturity and location 
               
               
                   
                   
                 of bud. Length is in the range of 0.5- 
               
               
                   
                   
                 4 cm and color is 144A. 
               
               
                   
                 Staminate shape 
                 N/A 
               
               
                   
                 Sepal color (RHS 
                 N/A 
               
               
                   
                 number) 
                   
               
               
                   
                 Pollen description 
                 N/A 
               
               
                   
                 Seed description 
                 Light brown, oval, solitary, 3-7 mm 
               
               
                   
                   
                 in length. Typically weigh .01-.03 g. 
               
               
                   
                   
                 Variation observed due to 
               
               
                   
                   
                 environmental conditions. 
               
               
                   
                 Marbling of seed 
                 Minimal. 
               
               
                   
                 Petal description 
                 N/A, Apetalous 
               
               
                   
                   
               
               
                   
                   
                 Parental Variety (CC)  
               
               
                   
                 Characteristics 
                 (Female Plant) 
               
               
                   
                   
               
               
                   
                 Flowering 
                 Dioecious, so grows pistillate 
               
               
                   
                 (blooming) habit 
                 flowers at nodes. Once branching 
               
               
                   
                   
                 also occurs at the node, the flower 
               
               
                   
                   
                 will grow above the branch before 
               
               
                   
                   
                 producing a flower. Flowers grow 
               
               
                   
                   
                 very bulbous, almost spherical. 
               
               
                   
                   
                 Leaves surrounding the buds 
               
               
                   
                   
                 radiate and are more present 
               
               
                   
                   
                 around apical buds. 
               
               
                   
                 Proportion of 
                 50% grown from seed, normal 
               
               
                   
                 female plants 
                 dioecious plant 
               
               
                   
                 Inflorescence 
                 Above 
               
               
                   
                 Position 
                   
               
               
                   
                 Flower 
                 Overlapping, congested, individual 
               
               
                   
                 arrangement 
                 flowers grow in spherical 
               
               
                   
                   
                 formation. Flowers are stacked or 
               
               
                   
                   
                 clustered at maturity. 
               
               
                   
                 Number of 
                 Hundreds to thousands 
               
               
                   
                 Flowers per plant 
                   
               
               
                   
                 Flower shape 
                 Has mostly radial symmetry with 
               
               
                   
                   
                 leaves growing all around the 
               
               
                   
                   
                 flowers. Because of its separation 
               
               
                   
                   
                 from other buds, grows generally 
               
               
                   
                   
                 spherically. 
               
               
                   
                 Flower 
                 7-13 mm 
               
               
                   
                 (individual 
                   
               
               
                   
                 pistillate) length 
                   
               
               
                   
                 Flower 
                 2-13 cm 
               
               
                   
                 (compound cyme) 
                   
               
               
                   
                 diameter 
                   
               
               
                   
                 Corolla 
                 No defined corolla 
               
               
                   
                 Corolla Color 
                 N/A 
               
               
                   
                 (RHS number) 
                   
               
               
                   
                 Bract shape 
                 Broad /rounded base with long, 
               
               
                   
                   
                 tapering apex. Longer and more 
               
               
                   
                   
                 prominent than offspring ‘C2B’. 
               
               
                   
                   
                 Bracts are typically 3-4 mm and are 
               
               
                   
                   
                 covered in glandular trichomes. 
               
               
                   
                 Bract color (RHS 
                 150A 
               
               
                   
                 number) 
                   
               
               
                   
                 Bracteole shape 
                 Same as bract, further enclosed in 
               
               
                   
                 (general 
                 flower so hard to identify without 
               
               
                   
                 description) 
                 dissection. Roughly 0.5-1 mm in 
               
               
                   
                   
                 size; very small. 
               
               
                   
                 Bracteole color 
                 150A 
               
               
                   
                 (RHS number) 
                   
               
               
                   
                 Calyx Shape 
                 Not present 
               
               
                   
                 (general 
                   
               
               
                   
                 description) 
                   
               
               
                   
                 Calyx color (RHS 
                 N/A 
               
               
                   
                 number) 
                   
               
               
                   
                 Stigma shape 
                 Ovate shape with broad base and 
               
               
                   
                   
                 tapering apex with 1-2 styles 
               
               
                   
                   
                 protruding. Generally smaller and 
               
               
                   
                   
                 broader than offspring ‘C2B’. 
               
               
                   
                 Stigma length 
                 5-8 mm 
               
               
                   
                 Stigma color 
                 150A 
               
               
                   
                 (RHS number) 
                   
               
               
                   
                 Trichome shape 
                 Capitate stalked gland, stalk is 
               
               
                   
                   
                 shorter than ‘C2B’. In 
               
               
                   
                   
                 mature plants grow on flowers and 
               
               
                   
                   
                 petioles. 
               
               
                   
                 Cystolithic non- 
                 Present on leaves. 
               
               
                   
                 glandular 
                   
               
               
                   
                 Trichome color 
                 Immature: 142D 
               
               
                   
                 (RHS number) 
                 Mature (when ready to harvest): 
               
               
                   
                   
                 172A 
               
               
                   
                 Terminal Bud 
                 When flowering: More radial 
               
               
                   
                 shape 
                 symmetry from leaves growing 
               
               
                   
                   
                 without obstruction from stem. 
               
               
                   
                 Terminal Bud 
                 145B to 145D 
               
               
                   
                 color (RHS 
                   
               
               
                   
                 number) 
                   
               
               
                   
                 Pedicle 
                 Present but shorter than the 
               
               
                   
                   
                 ‘C2B’. Length is in the 
               
               
                   
                   
                 range of 0.25-2 cm and color is N144C. 
               
               
                   
                 Staminate shape 
                 N/A 
               
               
                   
                 Sepal color (RHS 
                 N/A 
               
               
                   
                 number) 
                   
               
               
                   
                 Pollen description 
                 N/A 
               
               
                   
                 Seed description 
                 Seeds are 2-3 mm in length. The 
               
               
                   
                   
                 seeds have vertical lines along the 
               
               
                   
                   
                 sides of the seed but only 3-5 per 
               
               
                   
                   
                 seed. The seed is a light brown 
               
               
                   
                   
                 with white lines that look like 
               
               
                   
                   
                 veins between some of the vertical lines. 
               
               
                   
                 Marbling of seed 
                 There is no marbling. 
               
               
                   
                 Petal description 
                 N/A 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE V 
               
               
                   
               
               
                 Other Characteristics 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Characteristics 
                 New Variety 
               
               
                   
               
               
                 Time period and 
                 Flower production is initiated when 
               
               
                 condition of 
                 plants are taken from vegetative 
               
               
                 flowering/blooming 
                 growth at 24-hour light and switched 
               
               
                   
                 to 12 hours of light followed by 12 
               
               
                   
                 hours of dark during what would be 
               
               
                   
                 the nocturnal period. Flowers are 
               
               
                   
                 mature typically 75 days after 
               
               
                   
                 flowering light cycle is initiated. 
               
               
                   
                 flowering light cycle is initiated. 
               
               
                 Proportion of 
                 None 
               
               
                 hermaphrodite 
                   
               
               
                 plants 
                   
               
               
                 Hardiness of plant 
                 Is cold tolerant and grows well on 
               
               
                   
                 several different, diverse nutrient 
               
               
                   
                 formulas. Grows in USDA hardiness 
               
               
                   
                 zones 4-11. 
               
               
                 Breaking action 
                 Above average, very sturdy. 
               
               
                 Seed Shattering 
                 Minimal 
               
               
                 Root rate after 
                 Has exhibited 100% success at new 
               
               
                 cutting/cloning 
                 root development after cloning, 
               
               
                   
                 Adventitious roots appear at 10-14 days. 
               
               
                 Total THC and 
                 Total CBD content: 8.24% 
               
               
                 CBD Content at 
                 Total THC content: 0.3% 
               
               
                 harvest maturity 
                 Total CBD:THC Ratio: 25:1 
               
               
                 Total CBC content 
                 Total CBC: 3.64% 
               
               
                 at harvest maturity 
                 (CBC = .22% 
               
               
                   
                 CBD:CBC:THC 
               
               
                   
               
               
                   
                 Parental Variety (CC) 
               
               
                 Characteristics 
                 (Female Plant) 
               
               
                   
               
               
                 Time period and 
                 Flower production is initiated 
               
               
                 condition of 
                 when plants are taken from 
               
               
                 flowering/blooming 
                 vegetative growth at 24-hour light 
               
               
                   
                 and switched to 12 hours of light 
               
               
                   
                 followed by 12 hours of dark 
               
               
                   
                 during what would be the 
               
               
                   
                 nocturnal period. Flowers mature 
               
               
                   
                 typically 75 days after the 
               
               
                 Proportion of 
                 None 
               
               
                 hermaphrodite 
                   
               
               
                 plants 
                   
               
               
                 Hardiness of plant 
                 Tolerant to salty soil and drought 
               
               
                   
                 conditions. Grows in USDA 
               
               
                   
                 hardiness zones 6-11. 
               
               
                 Breaking action 
                 Above average, very sturdy. 
               
               
                 Seed Shattering 
                 Minimal 
               
               
                 Root rate after 
                 Has exhibited 100% success at 
               
               
                 cutting/cloning 
                 new root development after 
               
               
                   
                 cloning. Adventitious roots appear 
               
               
                   
                 at 10-14 days. 
               
               
                 Total THC and 
                 Total CBD content: 15% 
               
               
                 CBD Content at 
                 Total THC content: 0.5% 
               
               
                 harvest maturity 
                 Total CBD:THC Ratio: 30:1 
               
               
                 Total CBC content 
                 Total CBC: ND 
               
               
                 at harvest maturity 
                 and CBCA = 3.9%) 
               
               
                   
                 Ratio = 25:9:1 
               
               
                   
               
            
           
         
       
     
     The botanical descriptions provided are generalizing from plants grown indoors in controlled conditions. Cannabinoids were measured by extracting flower samples in ethanol and running samples on a Shimadzu LC 2030 3D plus with PDA detector (HPLC/PDA) instrument calibrated with cannabinoid standards from Cerilliant Chemical. Total potential cannabinoid content is measured using a formula to account for decarboxylation of the acidic forms to allow for more accurate estimation. The formulas used are provided for convenience:
 
Total THC=THC+(THCA*(0.877));
 
Total CBD=CBD+(CBDA*(0.877));
 
Total CBC=CBC+(CBCA*(0.877)).
 
     The K father, similar to ‘C2B’, has alternate, palmately compound leaves with 3 to 5 leaflets when immature and up to 9 at maturity. The leaflets have jagged serrate margins with the tooth apex angled towards the leaflet apex and about 19 to 29 teeth per leaflet. The leaves with the petiole are about 12 to 24 cm long and the petiole alone is about 4.2 to 8 cm long. The middle largest leaflet is about 7 to 16 cm long and 1.5 to 2 cm wide for a 4:1 or 8:1 length to width ratio. Generally, the K variety has the potential to produce 4% to 6% CBD and 0.13% to 0.27% THC which is lower than ‘C2B’ and K has no detectable levels of CBC. Other physical differences are present between ‘C2B’ and the K father due to the different appearances between male and female  Cannabis  plants. For example, males produce pollen sacs, are more-stout and taller, and have thicker stalks while females produce buds that grow into inflorescences and are usually shorter. 
     When compared to the CC mother, ‘C2B’ is generally a taller, more-slender plant. The ‘C2B’ grows faster with longer internodes which allows for the plant to reach taller heights but does not have as many leaves nor buds. The leaves of ‘C2B’ themselves even show similarities to the growth of the plant, with CC having leaflets that are much broader and with a smaller length:width ratio. The ‘C2B’ leaves more closely resemble the father, K, rather than the mother, CC. ‘C2B’ generally grows much-longer and skinnier leaflets that contribute to its sparse appearance. On the margins, ‘C2B’ has 28-32 serrations whereas CC has only 23-25. Although both plants grow relatively solitary, non-overlapping buds, CC is bushier and has more leaves growing radially around the buds with the apical bud fully surrounded. ‘C2B’ has buds that only have one or two leaves surrounding it and the apical has leaves that radiate in different layers. The distinction in density of leaves is prominent because the ‘C2B’ has long internodes where leaves grow which produce more space between the already-skinny leaves. At nodes where branches protrude from the stem, CC has smooth junctions (see  FIG. 7 ), whereas ‘C2B’ has a bulge at the connection point (see  FIG. 6 ). 
     When compared to another  Cannabis sativa  Hemp variety, CW24, the ‘C2B’ grows much skinnier and with less foliage. The CW24 grows flowers that are clustered and stack on top of one another near the apex of each branch. The leaves are generally radiating around each bud and grow tall and erect with no bending at maturity. ‘C2B’, on the other hand, grows much more sparsely, with buds grown separately and spread out by large internodes.
     Growth conditions:
             Vegetative growth period.— 24 hour light continuously. 78° F. and 60% humidity.     Flower production period.— 12 hour light followed by 12 hour dark cycle repeating. 78° F. and 45% humidity.       
       Outdoor growth: ‘C2B’ was grown outdoors on a farm located in Sonoma County, Calif. The plants were planted as 30-day clones early in the season, Aug. 16, 2019, resulting in an early flowering stage and smaller adult plants. The ‘C2B’ plant has the potential to reach a maximum of 15 to 17 feet tall and 10 feet wide. Compared to indoor growth, the plant will be more robust and have a faster growth rate with wider leaves. When grown outdoors, the father, K, variety will reach 10 to 15 feet tall and 10 feet wide and the mother, CC, variety will reach 8 to 10 feet tall and 6 to 8 feet wide.   Vigor: Exhibits most-vigorous and rapid growth when transitioning between vegetative and flowering growth.   Coloration: Deep bluish green with a lighter, yellowish underside. Changes in coloration occur with nutrient deficiencies or other variation in growing maintenance.