Patent Publication Number: US-2023140941-A1

Title: Non-atp/catalytic site p38 mitogen activated protein kinase inhibitors

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application claims priority to, and the benefit of, U.S. Provisional Application No. 62/989,470, filed Mar. 13, 2020. The contents of this application are incorporated by reference herein in its entirety. 
    
    
     FIELD 
     The invention relates generally to compounds that are inhibitors of p38 Mitogen-Activated Protein Kinases (MAPKs) proteins, and more particularly, but not exclusively, to compounds that inhibit p38α MAPK protein by binding to a pocket near the ED substrate-docking site of p38α MAPK, and methods of using such compounds as treatments for disease. 
     BACKGROUND 
     p38 Mitogen-Activated Protein Kinases (MAPKs) contribute to pathogenesis of many diseases, but the currently available p38 catalytic inhibitors (e.g., SB203580) are poorly effective and cause toxicity possibly due to activity against non-inflammatory p38 isoforms (e.g., p38ß) and loss of p38α-dependent counter regulatory responses (e.g., MSK1/2). Accordingly, new therapeutics and methods of treatment are needed in the field both to address selective inhibition of p38α MAPK and to selectively block certain p38α MAPK functions to preserve critical counterregulatory and homeostatic functions with application for the treatment of inflammatory and oncologic diseases. 
     SUMMARY 
     The disclosure provides a compound of Formula B, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof: 
     
       
         
         
             
             
         
       
     
     wherein in Formula B: Ar and Ar 1  are independently selected from a mono- or polycyclic optionally substituted cycloalkyl, mono- or polycyclic optionally substituted heterocycloalkyl, mono- or polycyclic optionally substituted aryl, mono- or polycyclic optionally substituted arylalkyl, mono- or polycyclic optionally substituted heteroaryl, and mono- or polycyclic optionally substituted heteroarylalkyl; R 1  and R 2  are independently selected from hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted alkylheteroaryl, wherein R 1  and R 2  can optionally be joined to form a carbo- or heterocycle; L 1  and L 2  are linkers comprising independently one or more of a bond, —NR a —, —S—, —S(O)—, —S(O) 2 —, —O—, —CR a   2 —, —C(O)O—, —OC(O)—, —C(O)S—, —SC(O)—, —C(O)NR a —, —NR a C(O)—, —C(O)NR a SO 2 —, —SO 2 NR a  C(O)—, —OC(O)O—, —OC(O)S—, —SC(O)O—, —OC(O)NR a —, —NR a C(O)O—, —S(O) t N(R a )— (where t is 1 or 2), —N(R a )S(O) t — (where t is 1 or 2), disubstituted alkyl, disubstituted heteroalkyl, disubstituted alkenyl, disubstituted alkynyl, disubstituted cycloalkyl, disubstituted heterocycloalkyl, disubstituted aryl, disubstituted arylalkyl, disubstituted heteroaryl, and disubstituted heteroarylalkyl; wherein any optional substituent is independently selected at each occurrence from optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted arylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —OC(O)—R a , —SC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —C(O)SR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , —N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O)R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), and PO 3 (R a ) 2 ; and R a  is independently selected at each occurrence from hydrogen, optionally substituted alkyl, optionally substituted fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocycloalkyl, optionally substituted heterocycloalkylalkyl, optionally substituted heteroaryl, and optionally substituted heteroarylalkyl. 
     In some embodiments, an optional substituent is independently selected from R 3 , R 4 , RV, and R 6 , which are independently selected from hydrogen, halo, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted alkylheteroaryl, wherein any two of R 1 , R 2 , R 3 , R 4 , R 5 , and R 6  can optionally be joined to form a carbo- or heterocycle. In some embodiments, an optional substituent is independently selected from halogen, —OH, —OR a , —NH 2 , —NHR a , —NR a   2 , —CO 2 H, —CO 2 R a , —CO 2 NH 2 , —CO 2 NHR a , —CONH 2 , —CONHR a , —CONR a   2 , —CO 2 R a   2 , —SO 2 H, —SO 2 R a , —SO 2 NH 2 , —SO 2 NHR a , and —SO 2 NR a   2 ; and R a  is independently selected at each occurrence from hydrogen, optionally substituted alkyl, optionally substituted fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocycloalkyl, optionally substituted heterocycloalkylalkyl, optionally substituted heteroaryl, and optionally substituted heteroarylalkyl 
     In some embodiments, Ar 1  is an optionally substituted mono- or polycyclic aryl or optionally substituted mono- or polycyclic heteroaryl ring. In some embodiments, Ar 1  is disubstituted benzene or disubstituted naphthalene. In some embodiments, Ar is a mono- or polycyclic optionally substituted aryl, a mono- or polycyclic optionally substituted arylalkyl, a mono- or polycyclic optionally substituted heteroaryl, or a mono- or polycyclic optionally substituted heteroarylalkyl. In some embodiments, Ar is a 5 or 6 membered optionally substituted aryl or a 5 or 6 membered optionally substituted heteroaryl, wherein the L 1  and L 2  are connected to Ar in a 1,2, 1,3, or 1,4 substitution pattern. In some embodiments, Ar is 1,2 disubstituted, 1,3 disubstituted, or 1,4 disubstituted benzene, pyridine, pyrimidine, pyrazine, pyridazine, or triazine. In some embodiments, Ar is 1,2 disubstituted benzene, 1,3 disubstituted benzene, or 1,4 disubstituted benzene. In some embodiments, Ar is 2,3 disubstituted, 2,4 disubstituted, 2,5 disubstituted, 2,6 disubstituted, 3,4 disubstituted, or 3,5 disubstituted pyridine. In some embodiments, Ar is 2,4 disubstituted, 2,5 disubstituted, 4,5 disubstituted, or 4,6 disubstituted pyrimidine. In some embodiments, Ar is 1,2 disubstituted or 1,3 disubstituted furan, thiophene, pyrrole, thiazole, isothiazole, 1,2,3-thiadiazole, imidazole, oxazole, isoxazole, 1,2,3-oxadiazole, 1,3,4-oxadiazole, 1,2,4-oxadiazole, 1,2,3-triazole, 1,2,4-triazole, or pyrazole. In some embodiments, Ar is 2,4 disubstituted, 2,5 disubstituted, or 4,5 disubstituted thiazole. In some embodiments, Ar is 2,3 disubstituted, 2,4 disubstituted, 2,5 disubstituted, or 3,4 disubstituted thiophene. In some embodiments, Ar is 1,2 disubstituted, 1,3 disubstituted, 1,4 disubstituted, 1,5 disubstituted, 1,6 disubstituted, 1,7 disubstituted, or 1,8 disubstituted naphthalene, quinoline, isoquinoline, cinnoline, quinoxaline, phtalazine, pyridopyrazine, pteridine, pyridopyridazine, naphtyridine, carbazole, dibenzofuran, or quinazoline. In some embodiments, Ar is 1,2 disubstituted, 1,3 disubstituted, 1,4 disubstituted, 1,5 disubstituted, 1,6 disubstituted, or 1,7 disubstituted indole, benzoxazole, benzothiophene, benzimidazole, indazole, benzotriazole, pyrrolopyridine, triazolopyridine, purine, indolizine, pyrrolopyrimidine, pyrrolopyrazine, pyrrolopyrimine, pyrrolopyridazine, imidazopyridine, pyrazolopyridine, imidazopyridazine, or imidazopyrimidine. 
     In some embodiments, L 1  is a linker selected from —CH 2 —, —C(CH 3 ) 2 —, and —C(CH 2 CH 2 )—. In some embodiments, L 1  is —CH 2 —. In some embodiments, L 2  is a linker selected from —NHCH 2 —, —CH 2 NH—, —NHCO—, —CONH—, —SO 2 NH—, and —NHSO 2 —. 
     In some embodiments, —NR 1 R 2  is selected from: 
     
       
         
         
             
             
         
       
     
     In some embodiments, R 7  is hydrogen or an optionally substituted alkyl. 
     In some embodiments, Ar 2  is a heterocycle. 
     In some embodiments, —NR 1 R 2  is 
     
       
         
         
             
             
         
       
     
     In some embodiments, Ar 1  is substituted by a —NR 8 R 9  group, wherein R 8  and R 9  are independently selected from hydrogen and optionally substituted alkyl. In some embodiments, R 8  and R 9  are independently methyl. 
     In some embodiments, Ar 1  is substituted by a halo substituent. 
     The disclosure also provides a compound of any one of Formulas I-a to XIII-a, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof: 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     wherein in Formulas I-a to XIII-a: each of R 1 , R 2 , R 3 , R 4 , R 5 , and R 6  is independently selected from hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted alkylheteroaryl, wherein R 1  and R 2  can optionally be joined to form a carbo- or heterocycle; L 1  is a linker selected from —CH 2 —, —C(CH 3 ) 2 —, and —C(CH 2 CH 2 )—; L 2  is a linker selected from —NHCH 2 —, —CH 2 NH—, —NHCO—, —CONH—, —SO 2 NH—, and —NHSO 2 —; and Ar 1  is an optionally substituted aryl or optionally substituted heteroaryl ring. In some embodiments, the —NR 1 R 2  group in any one of Formulas I-a to XIII-a is selected from: 
     
       
         
         
             
             
         
       
     
     wherein R 7  is hydrogen or an optionally substituted alkyl, and Ar 2  is a heterocycle. In some embodiments, the —NR 1 R 2  group in any one of Formulas I-a to XIII-a is selected from: 
     
       
         
         
             
             
         
       
     
     In some embodiments, the —NR 1 R 2  group in any one of Formulas I-a to XIII-a is 
     
       
         
         
             
             
         
       
     
     In some embodiments, L 1  is —CH 2 —. In some embodiments, each of R 3 , R 4 , R 5 , and R 6  are independently selected from hydrogen, halo, and haloalkyl. In some embodiments, R 3 , R 4 , R 5 , and R 6  are hydrogens. In some embodiments, L 2  is selected from —NHCH 2 —, —NHCO—, and —NHSO 2 —. In some embodiments, Ar 1  is disubstituted benzene or disubstituted naphthalene. In some embodiments, Ar 1  is selected from 
     
       
         
         
             
             
         
       
     
     In some embodiments, Ar 1  is selected from 
     
       
         
         
             
             
         
       
     
     The disclosure also provides a compound of any one of Formulas 2001 to 2007: 
     
       
         
         
             
             
         
       
     
     The disclosure also provides a compound of any one of Formulas 2008 to 2020: 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     The disclosure provides a compound of any of formula B, formula I-a to XIII-a, or formula 2001 to 2020, wherein the compound is a p38α MAPK inhibitor. In some embodiments, the p38α MAPK inhibitor is capable of binding to a pocket near the ED substrate-docking site of p38α MAPK and defined at least by residues R49, H107, L108, and K165 in p38α MAPK. In some embodiments, the binding pocket is defined by residues R49, H107, L108, M109, G110, A157, V158, E163, L164, and K165 in p38α MAPK. In some embodiments, the p38α MAPK inhibitor is a p38α MAPK selective inhibitor. 
     The disclosure also provides a pharmaceutical composition comprising a compound of any of formula B, formula I-a to XIII-a, or formula 2001 to 2020, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, and a physiologically compatible carrier medium, wherein the amount of compound in the composition is a therapeutically effective amount for the treatment or prevention of a disease alleviated by inhibiting p38α MAPK activity in a patient in need thereof. In some embodiments, the disease is cancer or an inflammatory disease. In some embodiments, the disease is selected from the group consisting of rheumatoid arthritis, a cardiovascular disease, multiple sclerosis, inflammatory bowel disease, chronic obstructive pulmonary disease (COPD), asthma, acute respiratory distress syndrome (ARDS), and acute lung injury (ALI). In some embodiments, the cancer is selected from the group consisting of acoustic neuroma, adenocarcinoma, angiosarcoma, astrocytoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, brain cancer, breast cancer, bronchogenic carcinoma, cervical cancer, chordoma, choriocarcinoma, colon cancer, colorectal cancer, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliocarcinoma, ependymoma, epithelial carcinoma, esophageal cancer, Ewing&#39;s tumor, fibrosarcoma, gastric cancer, glioblastoma multiforme, glioma, head and neck cancer, hemangioblastoma, hepatoma, kidney cancer, leiomyosarcoma, liposarcoma, lung cancer, lymphangioendotheliosarcoma, lymphangiosarcoma, medullary carcinoma, medulloblastoma, melanoma, meningioma, mesothelioma, myxosarcoma, nasal cancer, neuroblastoma, oligodendroglioma, oral cancer, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinoma, papillary carcinoma, pinealoma, prostate cancer, rabdomyosarcoma, rectal cancer, renal cell carcinoma, retinoblastoma, sarcoma, sebaceous gland carcinoma, seminoma, skin cancer, squamous cell carcinoma, stomach cancer, sweat gland carcinoma, synovioma, testicular cancer, small cell lung carcinoma, throat cancer, uterine cancer, Wilm&#39;s tumor, blood cancer, acute erythroleukemic leukemia, acute lymphoblastic B-cell leukemia, acute lymphoblastic T-cell leukemia, acute lymphoblastic leukemia, acute megakaryoblastic leukemia, acute monoblastic leukemia, acute myeloblastic leukemia, acute myelomonocytic leukemia, acute nonlymphocytic leukemia, acute promyelocytic leukemia, acute undifferentiated leukemia, chronic lymphocytic leukemia, chronic myelocytic leukemia, hairy cell leukemia, multiple myeloma, heavy chain disease, Hodgkin&#39;s disease, multiple myeloma, non-Hodgkin&#39;s lymphoma, polycythemia vera, and Waldenström&#39;s macroglobulinemia. 
     The disclosure also provides a method of treating or preventing a disease alleviated by inhibiting the p38α MAPK protein in a patient in need thereof, comprising administering to the patient a therapeutically effective amount of a p38α MAPK inhibitor, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, wherein the p38α MAPK inhibitor is a compound of any of formula B, formula I-a to XIII-a, or formula 2001 to 2020. The disclosure also provides a method of treating or preventing a disease alleviated by inhibiting the p38α MAPK protein in a patient in need thereof, comprising administering to the patient a therapeutically effective amount of a pharmaceutical composition comprising p38α MAPK inhibitor, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, wherein the p38α MAPK inhibitor is a compound of any of formula B, formula I-a to XIII-a, or formula 2001 to 2020, and a physiologically compatible carrier medium. In some embodiments, the p38α MAPK inhibitor is administered in a dosage unit form. In some embodiments, the dosage unit comprises a physiologically compatible carrier medium. In some embodiments, the disease is cancer or an inflammatory disease. In some embodiments, the disease is selected from the group consisting of rheumatoid arthritis, a cardiovascular disease, multiple sclerosis, inflammatory bowel disease, chronic obstructive pulmonary disease (COPD), asthma, acute respiratory distress syndrome (ARDS), and acute lung injury (ALI). In some embodiments, the cancer is selected from the group consisting of acoustic neuroma, adenocarcinoma, angiosarcoma, astrocytoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, brain cancer, breast cancer, bronchogenic carcinoma, cervical cancer, chordoma, choriocarcinoma, colon cancer, colorectal cancer, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliocarcinoma, ependymoma, epithelial carcinoma, esophageal cancer, Ewing&#39;s tumor, fibrosarcoma, gastric cancer, glioblastoma multiforme, glioma, head and neck cancer, hemangioblastoma, hepatoma, kidney cancer, leiomyosarcoma, liposarcoma, lung cancer, lymphangioendotheliosarcoma, lymphangiosarcoma, medullary carcinoma, medulloblastoma, melanoma, meningioma, mesothelioma, myxosarcoma, nasal cancer, neuroblastoma, oligodendroglioma, oral cancer, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinoma, papillary carcinoma, pinealoma, prostate cancer, rabdomyosarcoma, rectal cancer, renal cell carcinoma, retinoblastoma, sarcoma, sebaceous gland carcinoma, seminoma, skin cancer, squamous cell carcinoma, stomach cancer, sweat gland carcinoma, synovioma, testicular cancer, small cell lung carcinoma, throat cancer, uterine cancer, Wilm&#39;s tumor, blood cancer, acute erythroleukemic leukemia, acute lymphoblastic B-cell leukemia, acute lymphoblastic T-cell leukemia, acute lymphoblastic leukemia, acute megakaryoblastic leukemia, acute monoblastic leukemia, acute myeloblastic leukemia, acute myelomonocytic leukemia, acute nonlymphocytic leukemia, acute promyelocytic leukemia, acute undifferentiated leukemia, chronic lymphocytic leukemia, chronic myelocytic leukemia, hairy cell leukemia, multiple myeloma, heavy chain disease, Hodgkin&#39;s disease, multiple myeloma, non-Hodgkin&#39;s lymphoma, polycythemia vera, and Waldenström&#39;s macroglobulinemia. In some embodiments, the compound selectively inhibits p38α MAPK. In some embodiments, inhibition of p38α MAPK does not result in loss of a p38α-dependent counter-regulatory response. In some embodiments, the p38α-dependent counter regulatory-response relates to mitogen- and stress-activated protein kinase-1 (MSK1), or MSK2. In some embodiments, inhibiting p38α MAPK stabilizes an endothelial or epithelial barrier function. In some embodiments, inhibiting p38α MAPK reduces inflammation. In some embodiments, inhibiting p38α MAPK mitigates LPS-induced lung injury. In some embodiments, inhibiting p38α MAPK regulates leukocyte trafficking. In some embodiments, inhibiting p38α MAPK regulates cytokine expression. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       The foregoing summary, as well as the following detailed description of embodiments of the disclosure, will be better understood when read in conjunction with the appended drawings and figures. 
         FIG.  1    illustrates the design of substrate-selective p38 inhibitors.  FIG.  1   a    illustrates the structure of p38α showing CD, ED, DEF and activation site.  FIG.  1   b    illustrates the comparison between the p38α and β structures; CD and ED sites are colored red and blue, and the CADD target yellow. Sequence comprising the CADD target on p38α and corresponding site on p38β differ in only three of ten amino acids (highlighted yellow).  FIG.  1   c    illustrates the overlap of CADD target structure in apo- (PDB:1P38; green) and dual-phosphorylated (PDB:3PY3; yellow) mouse p38α.  FIG.  1   d    illustrates the overview of CADD screening strategy.  FIG.  1   e    illustrates the DSF screening of compounds added at 10, 25, 50, or 100 μM to recombinant p38α or ERK2 with binding indicated by increase in melting temperature. Compounds binding ERK2 and p38 α highlighted yellow. Those only binding to p38 α are highlighted in blue. FIG. if illustrates the chemical structure of UM60, UM101, and SB203580. 
         FIG.  2    illustrates the biological effects of p38 inhibitors.  FIG.  2   a    and  FIG.  2   b    illustrate the effect of 10 μM SB203580 (SB), or indicated concentration of UM60 or 101 on HMVECL permeability ( FIG.  2   a   ) and capacity for IL-8-directed neutrophil TEM ( FIG.  2   b   ). Cells were pretreated with DMSO or compounds for 1 h, then incubated with 10 ng/ml TNFα for 6 h prior to permeability assay ( FIG.  2   a   ) or at 39.5° C. without additional stimulus for 6 h prior to TEM assay ( FIG.  2   b   ). Mean±SE. * denotes p&lt;0.0001 vs. DMSO, † p&lt;0.0001 vs. SB, ‡ p&lt;0.005 vs. 37° C.  FIG.  2   c    and  FIG.  2   d   . Male CD1 mice were pretreated with 1 mg SB or 0.1-1 mg UM101 prior to i.t. instillation of 50 μg LPS and hyperthermia exposure. * denotes p&lt;0.05 vs. DMSO. 
         FIG.  3    illustrates the biochemical effects of substrate-selective p38 inhibitors.  FIG.  3   a    and  FIG.  3   b    illustrate the heat maps from RNASeq showing IPA pathways inhibited by SB203580 alone or SB203580 and UM101 or ( FIG.  3   a   ) and those only inhibited by UM101 ( FIG.  3   b   ).  FIG.  3   c    illustrate the biochemical effects of substrate-selective p38 inhibitors on HeLa cells pre-treated with 50 μM UM101 or 10 μM SB203580 (SB) for 30 min, then treated with anisomycin for 10-60 min, and immunoblotted for phospho-MK2, Stat-1 and total p38.  FIG.  3   d    illustrates the DSF analysis of UM101 and SB203580 (SB) binding to recombinant p38α and p38β. Mean±SE of 4 experiments. *, †, and § denote p&lt;0.0001 vs. p38α with DMSO, p38β with DMSO, and p38β with SB203580, respectively. P&lt;0.0001 for difference between UM101 binding to p38α and p38β by MANOVA.  FIG.  3   e    illustrates the DSF analysis of UM101 and SB203580 (SB) binding to recombinant wild-type p38α and a p38α mutant with 4 mutations in CADD-targeted pocket. Mean±SE of 4 experiments. * and †, denote p&lt;0.0001 vs. wild-type with DMSO and mutant with DMSO, respectively. P&lt;0.0001 for difference between UM101 binding to wild-type and mutant p38α by MANOVA.  FIG.  3   f - k    illustrate STD-NMR performed with UM101 and p38α ( FIG.  3   f    and  FIG.  3   g   ), p38β ( FIG.  3   h    and  FIG.  3   i   ), and the p38α mutant ( FIG.  3   j    and  FIG.  3   k   ). The 1D ( FIG.  3   f   ,  FIG.  3   h   , and  FIG.  3   j   ) and STD spectra ( FIG.  3   g   ,  FIG.  3   i   , and  FIG.  3   k   ) from the same sample are shown. The tentative peak assignments are indicated in  FIG.  3   f   . The structure of UM101 with the protons labeled is shown in the insert. 
         FIG.  4    illustrates the preliminary analysis of IL-8 and IL-18 mRNA by qRT-PCR prior to RNASeq. HMVECLs were preincubated with 0.4% DMSO, 10 μM SB203580, or 100 μM UM101 for 1 h, then stimulated with 10 ng/ml TNFα for 4 h and total RNA was collected, reverse transcribed, analyzed by qRT-PCR and fold change relative to unstimulated control cells calculated using the delta-delta method and GAPDH as the housekeeping gene. 
         FIG.  5    is a quadrant map of RNASeq analysis. Genes with at least 10 reads in one sample per set and at least 2-fold increase with TNFα are shown. The key refers to the direction of change in UM101-treated cells/SB203580-treated cells vs. DMSO-treated cells. 
         FIG.  6    is the SILCS FragMap of p38α. Nonpolar maps (green) indicate putative binding pockets, with the location of the ED site indicated. H-bond donor (blue) and acceptor (red) maps are shown. 
         FIG.  7    illustrates compound UM101 overlaid on SILCS FragMaps shown in wireframe at contours of −1.0 kcal/mol for aromatic (purple), aliphatic (green), positive (cyan), H-bond acceptor (red) and H-bond donor (blue) functional groups on the p38α backbone, with sidechains of the four residues mutated in our CADD-site-disrupted mutant indicated. Spatial distributions of FragMaps indicate where respective functional groups make favorable contributions to binding. 
         FIG.  8    illustrates that UM101 and analogues inhibit thrombin-induced cell permeability. Endothelial cells were pretreated with or without 10 or 15 mM of UM101, SF-6-222 (6222), or SF-7-009 (7009). Transepithelial/transendothelial electrical resistance (TEER) was used to measure cell permeability. 
         FIG.  9    illustrates that UM101 and analogues inhibit acute lung injury in a mouse model. Mice were pretreated i.p. with 1 mg of test compounds and then treated with 100 mg LPS i.t. at 39° C. to induce inflammation. Lung injury was assessed by measuring total protein in the lung lavage fluid. 
         FIG.  10    illustrates that UM101 and analogues inhibit acute lung injury in a mouse model. All mice received 50 μg LPS i.t. and were kept 24 h at 39° C.; all treatments 1 mg in 0.5 ml DMSO given 6 h post-LPS; mean±SM, n=5, * p=0.03 vs DMSO #1; † p=0.16 vs DMSO #2. Lung injury was assessed by measuring total protein in the lung lavage fluid. 
     
    
    
     DETAILED DESCRIPTION 
     Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this disclosure belongs. All patents and publications referred to herein are incorporated by reference in their entireties. 
     Definitions 
     As used herein, the terms “administer,” “administration” or “administering” refer to (1) providing, giving, dosing, and/or prescribing by either a health practitioner or his authorized agent or under his or her direction according to the disclosure; and/or (2) putting into, taking or consuming by the mammal, according to the disclosure. 
     The terms “co-administration,” “co-administering,” “administered in combination with,” “administering in combination with,” “simultaneous,” and “concurrent,” as used herein, encompass administration of two or more active pharmaceutical ingredients to a subject so that both active pharmaceutical ingredients and/or their metabolites are present in the subject at the same time. Co-administration includes simultaneous administration in separate compositions, administration at different times in separate compositions, or administration in a composition in which two or more active pharmaceutical ingredients are present. Simultaneous administration in separate compositions and administration in a composition in which both agents are present are preferred. 
     The terms “active pharmaceutical ingredient” and “drug” include the p38α MAPK inhibitors described herein and, more specifically, the p38α MAPK inhibitors described by formulas A, B, I, II, I-a to XIII-a, 1001-1180, e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044), and formulas 2001-2020. The terms “active pharmaceutical ingredient” and “drug” may also include those compounds described herein that bind p38α MAPK protein and thereby modulate p38α MAPK protein activity. 
     The term “isostere” refers to a group or molecule whose chemical and/or physical properties are similar to those of another group or molecule. A “bioisostere” is a type of isostere and refers to a group or molecule whose biological properties are similar to those of another group or molecule. For example, for the p38α MAPK inhibitors described herein, a carboxylic acid may be replaced by one of the following bioisosteres for carboxylic acids, including, without limitation, alkyl esters (COOR), acylsulfonamides (CONR—SO 2 R), hydroxamic acids (CONR—OH), hydroxamates (CONR—OR), tetrazoles, hydroxyisoxazoles, isoxazol-3-ones, and sulfonamides (SO 2 NR), where each R may independently represent hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     The term “in vivo” refers to an event that takes place in a subject&#39;s body. 
     The term “in vitro” refers to an event that takes places outside of a subject&#39;s body. In vitro assays encompass cell-based assays in which cells alive or dead are employed and may also encompass a cell-free assay in which no intact cells are employed. 
     The term “effective amount” or “therapeutically effective amount” refers to that amount of a compound or combination of compounds as described herein that is sufficient to effect the intended application including, but not limited to, disease treatment. A therapeutically effective amount may vary depending upon the intended application (in vitro or in vivo), or the subject and disease condition being treated (e.g., the weight, age and gender of the subject), the severity of the disease condition, the manner of administration, etc., which can readily be determined by one of ordinary skill in the art. The term also applies to a dose that will induce a particular response in target cells (e.g., the reduction of platelet adhesion and/or cell migration). The specific dose will vary depending on the particular compounds chosen, the dosing regimen to be followed, whether the compound is administered in combination with other compounds, timing of administration, the tissue to which it is administered, and the physical delivery system in which the compound is carried. 
     A “therapeutic effect” as that term is used herein, encompasses a therapeutic benefit and/or a prophylactic benefit. A prophylactic effect includes delaying or eliminating the appearance of a disease or condition, delaying or eliminating the onset of symptoms of a disease or condition, slowing, halting, or reversing the progression of a disease or condition, or any combination thereof. 
     As used herein, the terms “treat,” “treatment,” and/or “treating” may refer to the management of a disease, disorder, or pathological condition, or symptom thereof with the intent to cure, ameliorate, stabilize, and/or control the disease, disorder, pathological condition or symptom thereof. Regarding control of the disease, disorder, or pathological condition more specifically, “control” may include the absence of condition progression, as assessed by the response to the methods recited herein, where such response may be complete (e.g., placing the disease in remission) or partial (e.g., lessening or ameliorating any symptoms associated with the condition). As used herein, the terms “prevent,” “preventing,” and/or “prevention” may refer to reducing the risk of developing a disease, disorder, or pathological condition. 
     As used herein, the terms “modulate” and “modulation” refer to a change in biological activity for a biological molecule (e.g., a protein, gene, peptide, antibody, and the like), where such change may relate to an increase in biological activity (e.g., increased activity, agonism, activation, expression, upregulation, and/or increased expression) or decrease in biological activity (e.g., decreased activity, antagonism, suppression, deactivation, downregulation, and/or decreased expression) for the biological molecule. For example, the compounds described herein may modulate (i.e., inhibit) p38α MAPK protein. In some embodiments, the compounds described herein may selectively modulate (i.e., selectively inhibit) p38α MAPK protein as compared to other MAPK or p38 MAPK proteins. In some embodiments, the compounds described herein may selectively modulate (i.e., selectively inhibit) p38α MAPK protein as compared to other MAPK or p38 MAPK proteins. 
     The terms “QD,” “qd,” or “q.d.” mean quaque die, once a day, or once daily. The terms “BID,” “bid,” or “b.i.d.” mean bis in die, twice a day, or twice daily. The terms “TID,” “tid,” or “t.i.d.” mean ter in die, three times a day, or three times daily. The terms “QID,” “qid,” or “q.i.d.” mean quater in die, four times a day, or four times daily. 
     The term “pharmaceutically acceptable salt” refers to salts derived from a variety of organic and inorganic counter ions known in the art. Pharmaceutically acceptable acid addition salts can be formed with inorganic acids and organic acids. Preferred inorganic acids from which salts can be derived include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid and phosphoric acid. Preferred organic acids from which salts can be derived include, for example, acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid and salicylic acid. Pharmaceutically acceptable base addition salts can be formed with inorganic and organic bases. Inorganic bases from which salts can be derived include, for example, sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese and aluminum. Organic bases from which salts can be derived include, for example, primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins. Specific examples include isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, and ethanolamine. In some embodiments, the pharmaceutically acceptable base addition salt is chosen from ammonium, potassium, sodium, calcium, and magnesium salts. The term “cocrystal” refers to a molecular complex derived from a number of cocrystal formers known in the art. Unlike a salt, a cocrystal typically does not involve hydrogen transfer between the cocrystal and the drug, and instead involves intermolecular interactions, such as hydrogen bonding, aromatic ring stacking, or dispersive forces, between the cocrystal former and the drug in the crystal structure. 
     “Pharmaceutically acceptable carrier” or “pharmaceutically acceptable excipient” or “physiologically compatible” carrier or carrier medium is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and inert ingredients. The use of such pharmaceutically acceptable carriers or pharmaceutically acceptable excipients for active pharmaceutical ingredients is well known in the art. Except insofar as any conventional pharmaceutically acceptable carrier or pharmaceutically acceptable excipient is incompatible with the active pharmaceutical ingredient, its use in the therapeutic compositions of the disclosure is contemplated. Additional active pharmaceutical ingredients, such as other drugs, can also be incorporated into the described compositions and methods. 
     A “prodrug” refers to a derivative of a compound described herein, the pharmacologic action of which results from the conversion by chemical or metabolic processes in vivo to the active compound. Prodrugs include compounds wherein an amino acid residue, or a polypeptide chain of two or more (e.g., two, three or four) amino acid residues is covalently joined through an amide or ester bond to a free amino, hydroxyl or carboxylic acid group of a compound of any one of formulas A, B, I, II, I-a to XIII-a, 1001-1180, e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044), and formulas 2001-2020. The amino acid residues include but are not limited to the 20 naturally occurring amino acids commonly designated by one or three letter symbols but also include, for example, 4-hydroxyproline, hydroxylysine, desmosine, isodesmosine, 3-methylhistidine, beta-alanine, gamma-aminobutyric acid, citrulline, homocysteine, homoserine, omithine and methionine sulfone. Additional types of prodrugs are also encompassed. For instance, free carboxyl groups can be derivatized as amides or alkyl esters (e.g., methyl esters and acetoxy methyl esters). Prodrug esters as employed herein includes esters and carbonates formed by reacting one or more hydroxyls of compounds of the method of the disclosure with alkyl, alkoxy, or aryl substituted acylating agents employing procedures known to those skilled in the art to generate acetates, pivalates, methylcarbonates, benzoates and the like. As further examples, free hydroxyl groups may be derivatized using groups including but not limited to hemisuccinates, phosphate esters, dimethylaminoacetates, and phosphoryloxymethyloxycarbonyls, as outlined in Advanced Drug Delivery Reviews, 1996, 19, 115. Carbamate prodrugs of hydroxyl and amino groups are also included, as are carbonate prodrugs, sulfonate prodrugs, sulfonate esters and sulfate esters of hydroxyl groups. Free amines can also be derivatized to amides, sulfonamides or phosphonamides. All of the stated prodrug moieties may incorporate groups including but not limited to ether, amine and carboxylic acid functionalities. Moreover, any compound that can be converted in vivo to provide the bioactive agent (e.g., a compound of any one of formulas A, B, I, II, I-a to XIII-a, 1001-1180, e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044), and formulas 2001-2020) is a prodrug within the scope of the disclosure. Various forms of prodrugs are well known in the art. A comprehensive description of pro drugs and prodrug derivatives are described in: (a) The Practice of Medicinal Chemistry, Camille G. Wermuth et al., (Academic Press, 1996); (b) Design of Prodrugs, edited by H. Bundgaard, (Elsevier, 1985); (c) A Textbook of Drug Design and Development, P. Krogsgaard-Larson and H. Bundgaard, eds., (Harwood Academic Publishers, 1991). In general, prodrugs may be designed to improve the penetration of a drug across biological membranes in order to obtain improved drug absorption, to prolong duration of action of a drug (slow release of the parent drug from a prodrug, decreased first-pass metabolism of the drug), to target the drug action (e.g., organ or tumor-targeting, lymphocyte targeting), to modify or improve aqueous solubility of a drug (e.g., i.v. preparations and eyedrops), to improve topical drug delivery (e.g., dermal and ocular drug delivery), to improve the chemical/enzymatic stability of a drug, or to decrease off-target drug effects, and more generally in order to improve the therapeutic efficacy of the compounds utilized in the disclosure. 
     Unless otherwise stated, the chemical structures depicted herein are intended to include compounds which differ only in the presence of one or more isotopically enriched atoms. For example, compounds where one or more hydrogen atoms is replaced by deuterium or tritium, or wherein one or more carbon atoms is replaced by  13 C- or  14 C-enriched carbons, are within the scope of this disclosure. 
     When ranges are used herein to describe, for example, physical or chemical properties such as molecular weight or chemical formulae, all combinations and subcombinations of ranges and specific embodiments therein are intended to be included. Use of the term “about” when referring to a number or a numerical range means that the number or numerical range referred to is an approximation within experimental variability (or within statistical experimental error), and thus the number or numerical range may vary. The variation is typically from 0% to 15%, or from 0% to 10%, or from 0% to 5% of the stated number or numerical range. The term “comprising” (and related terms such as “comprise” or “comprises” or “having” or “including”) includes those embodiments such as, for example, an embodiment of any composition of matter, method or process that “consist of” or “consist essentially of” the described features. 
     “Alkyl” refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, containing no unsaturation, having from one to ten carbon atoms (e.g., (C 1-10 )alkyl or C 1-10  alkyl). Whenever it appears herein, a numerical range such as “i to 10” refers to each integer in the given range, e.g., “i to 10 carbon atoms” means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc., up to and including 10 carbon atoms, although the definition is also intended to cover the occurrence of the term “alkyl” where no numerical range is specifically designated. Typical alkyl groups include, but are in no way limited to, methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, sec-butyl isobutyl, tertiary butyl, pentyl, isopentyl, neopentyl, hexyl, septyl, octyl, nonyl and decyl. The alkyl moiety may be attached to the rest of the molecule by a single bond, such as for example, methyl (Me), ethyl (Et), n-propyl (Pr), 1-methylethyl (isopropyl), n-butyl, n-pentyl, 1,1-dimethylethyl (t-butyl) and 3-methylhexyl. Unless stated otherwise specifically in the specification, an alkyl group is optionally substituted by one or more of substituents which are independently heteroalkyl, acylsulfonamido, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2  where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Alkylaryl” refers to an -(alkyl)aryl radical where aryl and alkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for aryl and alkyl respectively. 
     “Alkylhetaryl” refers to an -(alkyl)hetaryl radical where hetaryl and alkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for aryl and alkyl respectively. 
     “Alkylheterocycloalkyl” refers to an -(alkyl) heterocyclic radical where alkyl and heterocycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heterocycloalkyl and alkyl respectively. 
     An “alkene” moiety refers to a group consisting of at least two carbon atoms and at least one carbon-carbon double bond, and an “alkyne” moiety refers to a group consisting of at least two carbon atoms and at least one carbon-carbon triple bond. The alkyl moiety, whether saturated or unsaturated, may be branched, straight chain, or cyclic. 
     “Alkenyl” refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one double bond, and having from two to ten carbon atoms (i.e., (C 2-10 )alkenyl or C 2-10  alkenyl). Whenever it appears herein, a numerical range such as “2 to 10” refers to each integer in the given range—e.g., “2 to 10 carbon atoms” means that the alkenyl group may consist of 2 carbon atoms, 3 carbon atoms, etc., up to and including 10 carbon atoms. The alkenyl moiety may be attached to the rest of the molecule by a single bond, such as for example, ethenyl (i.e., vinyl), prop-1-enyl (i.e., allyl), but-1-enyl, pent-1-enyl and penta-1,4-dienyl. Unless stated otherwise specifically in the specification, an alkenyl group is optionally substituted by one or more substituents which are independently alkyl, heteroalkyl, acylsulfonamido, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Alkenyl-cycloalkyl” refers to an -(alkenyl)cycloalkyl radical where alkenyl and cycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for alkenyl and cycloalkyl respectively. 
     “Alkynyl” refers to a straight or branched hydrocarbon chain radical group consisting solely of carbon and hydrogen atoms, containing at least one triple bond, having from two to ten carbon atoms (i.e., (C 2-10 )alkynyl or C 2-10  alkynyl). Whenever it appears herein, a numerical range such as “2 to 10” refers to each integer in the given range—e.g., “2 to 10 carbon atoms” means that the alkynyl group may consist of 2 carbon atoms, 3 carbon atoms, etc., up to and including 10 carbon atoms. The alkynyl may be attached to the rest of the molecule by a single bond, for example, ethynyl, propynyl, butynyl, pentynyl and hexynyl. Unless stated otherwise specifically in the specification, an alkynyl group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, acylsulfonamido, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Alkynyl-cycloalkyl” refers to an -(alkynyl)cycloalkyl radical where alkynyl and cycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for alkynyl and cycloalkyl respectively. 
     “Acylsulfonamide” refers to the group —C(═O)NR a —S(═O) 2 R a , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl, or heteroarylalkyl. 
     “Carboxaldehyde” refers to a —(C═O)H radical. 
     “Carbonyl” refers to the group —C(═O)—. Carbonyl groups may be substituted with the following exemplary substituents: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, acylsulfonamido, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —NR a —OR a —, —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Carboxyl” refers to a —(C═O)OH radical. 
     “Cyano” refers to a —CN radical. 
     “Cycloalkyl” refers to a monocyclic or polycyclic radical that contains only carbon and hydrogen, and may be saturated, or partially unsaturated. Cycloalkyl groups include groups having from 3 to 10 ring atoms (i.e., (C 3-10 )cycloalkyl or C 3-10  cycloalkyl). Whenever it appears herein, a numerical range such as “3 to 10” refers to each integer in the given range—e.g., “3 to 10 carbon atoms” means that the cycloalkyl group may consist of 3 carbon atoms, etc., up to and including 10 carbon atoms. Illustrative examples of cycloalkyl groups include, but are not limited to the following moieties: cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, norbomyl, and the like. Unless stated otherwise specifically in the specification, a cycloalkyl group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, acylsulfonamido, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —S(O)R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O)R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Cycloalkyl-alkenyl” refers to a -(cycloalkyl)alkenyl radical where cycloalkyl and alkenyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for cycloalkyl and alkenyl, respectively. 
     “Cycloalkyl-heterocycloalkyl” refers to a -(cycloalkyl)heterocycloalkyl radical where cycloalkyl and heterocycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for cycloalkyl and heterocycloalkyl, respectively. 
     “Cycloalkyl-heteroaryl” refers to a -(cycloalkyl)heteroaryl radical where cycloalkyl and heteroaryl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for cycloalkyl and heteroaryl, respectively. 
     The term “alkoxy” refers to the group —O-alkyl, including from 1 to 8 carbon atoms of a straight, branched, cyclic configuration and combinations thereof attached to the parent structure through an oxygen. Examples include, but are not limited to, methoxy, ethoxy, propoxy, isopropoxy, cyclopropyloxy and cyclohexyloxy. “Lower alkoxy” refers to alkoxy groups containing one to six carbons. 
     The term “substituted alkoxy” refers to alkoxy wherein the alkyl constituent is substituted (i.e., —O-(substituted alkyl)). Unless stated otherwise specifically in the specification, the alkyl moiety of an alkoxy group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, acylsulfonamido, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     The term “alkoxycarbonyl” refers to a group of the formula (alkoxy)(C═O)-attached through the carbonyl carbon wherein the alkoxy group has the indicated number of carbon atoms. Thus a (C 1-6 )alkoxycarbonyl group is an alkoxy group having from 1 to 6 carbon atoms attached through its oxygen to a carbonyl linker. “Lower alkoxycarbonyl” refers to an alkoxycarbonyl group wherein the alkoxy group is a lower alkoxy group. 
     The term “substituted alkoxycarbonyl” refers to the group (substituted alkyl)-O—C(O)— wherein the group is attached to the parent structure through the carbonyl functionality. Unless stated otherwise specifically in the specification, the alkyl moiety of an alkoxycarbonyl group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, acylsulfonamido, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O)R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Acyl” refers to the groups (alkyl)-C(O)—, (aryl)-C(O)—, (heteroaryl)-C(O)—, (heteroalkyl)-C(O)— and (heterocycloalkyl)-C(O)—, wherein the group is attached to the parent structure through the carbonyl functionality. If the R radical is heteroaryl or heterocycloalkyl, the hetero ring or chain atoms contribute to the total number of chain or ring atoms. Unless stated otherwise specifically in the specification, the alkyl, aryl or heteroaryl moiety of the acyl group is optionally substituted by one or more substituents which are independently alkyl, heteroalkyl, acylsulfonamido, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O)R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Acyloxy” refers to a R(C═O)O— radical wherein R is alkyl, aryl, heteroaryl, heteroalkyl or heterocycloalkyl, which are as described herein. If the R radical is heteroaryl or heterocycloalkyl, the hetero ring or chain atoms contribute to the total number of chain or ring atoms. Unless stated otherwise specifically in the specification, the R of an acyloxy group is optionally substituted by one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O)R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Amino” or “amine” refers to a —N(R a ) 2  radical group, where each R is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl, unless stated otherwise specifically in the specification. When a —N(R a ) 2  group has two R a  substituents other than hydrogen, they can be combined with the nitrogen atom to form a 4-, 5-, 6- or 7-membered ring. For example, —N(R a ) 2  is intended to include, but is not limited to, 1-pyrrolidinyl and 4-morpholinyl. Unless stated otherwise specifically in the specification, an amino group is optionally substituted by one or more substituents which independently are: alkyl, acylsulfonamido, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O)R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     The term “substituted amino” also refers to N-oxides of the groups —NHR d , and NR d R d  each as described above. N-oxides can be prepared by treatment of the corresponding amino group with, for example, hydrogen peroxide or m-chloroperoxybenzoic acid. 
     “Amide” or “amido” refers to a chemical moiety with formula —C(O)NR a R b  or —NR a C(O)R b , where R a  and R b  are selected from the group consisting of hydrogen, alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon), each of which moiety may itself be optionally substituted. The R a  and R b  of —C(O)N R a R b  amide may optionally be taken together with the nitrogen to which they are attached to form a 4-, 5-, 6- or 7-membered ring. Unless stated otherwise specifically in the specification, an amido group is optionally substituted independently by one or more of the substituents as described herein for alkyl, amino, cycloalkyl, aryl, heteroaryl, or heterocycloalkyl. An amide may be an amino acid or a peptide molecule attached to a compound disclosed herein, thereby forming a prodrug. The procedures and specific groups to make such amides are known to those of skill in the art and can readily be found in seminal sources such as Greene and Wuts,  Protective Groups in Organic Synthesis,  3 rd  Ed., John Wiley &amp; Sons, New York, N.Y., 1999, which is incorporated herein by reference in its entirety. 
     “Aromatic” or “aryl” or “Ar” refers to an aromatic radical with six to ten ring atoms (e.g., C 6 -C 10  aromatic or C 6 -C 10  aryl) which has at least one ring having a conjugated pi electron system which is carbocyclic (e.g., phenyl, fluorenyl, and naphthyl). Bivalent radicals formed from substituted benzene derivatives and having the free valences at ring atoms are named as substituted phenylene radicals. Bivalent radicals derived from univalent polycyclic hydrocarbon radicals whose names end in “-yl” by removal of one hydrogen atom from the carbon atom with the free valence are named by adding “-idene” to the name of the corresponding univalent radical, e.g., a naphthyl group with two points of attachment is termed naphthylidene. Whenever it appears herein, a numerical range such as “6 to 10” refers to each integer in the given range; e.g., “6 to 10 ring atoms” means that the aryl group may consist of 6 ring atoms, 7 ring atoms, etc., up to and including 10 ring atoms. The term includes monocyclic or fused-ring polycyclic (i.e., rings which share adjacent pairs of ring atoms) groups. Unless stated otherwise specifically in the specification, an aryl moiety is optionally substituted by one or more substituents which are independently alkyl, heteroalkyl, acylsulfonamido, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(O)R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Aralkyl” or “arylalkyl” refers to an (aryl)alkyl-radical where aryl and alkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for aryl and alkyl respectively. 
     “Ester” refers to a chemical radical of formula —COOR, where R is selected from the group consisting of alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon). The procedures and specific groups to make esters are known to those of skill in the art and can readily be found in seminal sources such as Greene and Wuts, Protective Groups in Organic Synthesis, 3 rd  Ed., John Wiley &amp; Sons, New York, N.Y., 1999, which is incorporated herein by reference in its entirety. Unless stated otherwise specifically in the specification, an ester group is optionally substituted by one or more substituents which independently are: alkyl, acylsulfonamido, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —S(OW)—R a  (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Fluoroalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more fluoro radicals, as defined above, for example, trifluoromethyl, difluoromethyl, 2,2,2-trifluoroethyl, 1-fluoromethyl-2-fluoroethyl, and the like. The alkyl part of the fluoroalkyl radical may be optionally substituted as defined above for an alkyl group. 
     “Halo,” “halide,” or, alternatively, “halogen” is intended to mean fluoro, chloro, bromo or iodo. The terms “haloalkyl,” “haloalkenyl,” “haloalkynyl,” and “haloalkoxy” include alkyl, alkenyl, alkynyl and alkoxy structures that are substituted with one or more halo groups or with combinations thereof. For example, the terms “fluoroalkyl” and “fluoroalkoxy” include haloalkyl and haloalkoxy groups, respectively, in which the halo is fluorine. 
     “Heteroalkyl,” “heteroalkenyl,” and “heteroalkynyl” refer to optionally substituted alkyl, alkenyl and alkynyl radicals and which have one or more skeletal chain atoms selected from an atom other than carbon, e.g., oxygen, nitrogen, sulfur, phosphorus or combinations thereof. A numerical range may be given—e.g., C 1 -C 4  heteroalkyl which refers to the chain length in total, which in this example is 4 atoms long. A heteroalkyl group may be substituted with one or more substituents which independently are: alkyl, heteroalkyl, alkenyl, alkynyl, acylsulfonamido, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, nitro, oxo, thioxo, trimethylsilanyl, —OR a , —SR a , —S(O) t R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR) 2 , where each R is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Heteroalkylaryl” refers to an -(heteroalkyl)aryl radical where heteroalkyl and aryl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heteroalkyl and aryl, respectively. 
     “Heteroalkylheteroaryl” refers to an -(heteroalkyl)heteroaryl radical where heteroalkyl and heteroaryl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heteroalkyl and heteroaryl, respectively. 
     “Heteroalkylheterocycloalkyl” refers to an -(heteroalkyl)heterocycloalkyl radical where heteroalkyl and heterocycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heteroalkyl and heterocycloalkyl, respectively. 
     “Heteroalkylcycloalkyl” refers to an -(heteroalkyl)cycloalkyl radical where heteroalkyl and cycloalkyl are as disclosed herein and which are optionally substituted by one or more of the substituents described as suitable substituents for heteroalkyl and cycloalkyl, respectively. 
     “Heteroaryl” or “heteroaromatic” or “HetAr” refers to a 5- to 18-membered aromatic radical (e.g., C5-C13 heteroaryl) that includes one or more ring heteroatoms selected from nitrogen, oxygen and sulfur, and which may be a monocyclic, bicyclic, tricyclic or tetracyclic ring system. Whenever it appears herein, a numerical range such as “5 to 18” refers to each integer in the given range—e.g., “5 to 18 ring atoms” means that the heteroaryl group may consist of 5 ring atoms, 6 ring atoms, etc., up to and including 18 ring atoms. Bivalent radicals derived from univalent heteroaryl radicals whose names end in “-yl” by removal of one hydrogen atom from the atom with the free valence are named by adding “-idene” to the name of the corresponding univalent radical—e.g., a pyridyl group with two points of attachment is a pyridylidene. A N-containing “heteroaromatic” or “heteroaryl” moiety refers to an aromatic group in which at least one of the skeletal atoms of the ring is a nitrogen atom. The polycyclic heteroaryl group may be fused or non-fused. The heteroatom(s) in the heteroaryl radical are optionally oxidized. One or more nitrogen atoms, if present, are optionally quaternized. The heteroaryl may be attached to the rest of the molecule through any atom of the ring(s). Examples of heteroaryls include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzindolyl, 1,3-benzodioxolyl, benzofuranyl, benzooxazolyl, benzo[d]thiazolyl, benzothiadiazolyl, benzo[b][1,4]dioxepinyl, benzo[b][1,4]oxazinyl, 1,4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzoxazolyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranonyl, benzofurazanyl, benzothiazolyl, benzothienyl(benzothiophenyl), benzothieno[3,2-d]pyrimidinyl, benzotriazolyl, benzo[4,6]imidazo[1,2-a]pyridinyl, carbazolyl, cinnolinyl, cyclopenta[d]pyrimidinyl, 6,7-dihydro-5H-cyclopenta[4,5]thieno[2,3-d]pyrimidinyl, 5,6-dihydrobenzo[h]quinazolinyl, 5,6-dihydrobenzo[h]cinnolinyl, 6,7-dihydro-5H-benzo[6,7]cyclohepta[1,2-c]pyridazinyl, dibenzofuranyl, dibenzothiophenyl, furanyl, furazanyl, furanonyl, furo[3,2-c]pyridinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyrimidinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyridazinyl, 5,6,7,8,9,10-hexahydrocycloocta[d]pyridinyl, isothiazolyl, imidazolyl, indazolyl, indolyl, indazolyl, isoindolyl, indolinyl, isoindolinyl, isoquinolyl, indolizinyl, isoxazolyl, isoxazol-3-one, 5,8-methano-5,6,7,8-tetrahydroquinazolinyl, naphthyridinyl, 1,6-naphthyridinonyl, oxadiazolyl, 2-oxoazepinyl, oxazolyl, oxiranyl, 5,6,6a,7,8,9,10,10a-octahydrobenzo[h]quinazolinyl, 1-phenyl-1H-pyrrolyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, pyranyl, pyrrolyl, pyrazolyl, pyrazolo[3,4-d]pyrimidinyl, pyridinyl, pyrido[3,2-d]pyrimidinyl, pyrido[3,4-d]pyrimidinyl, pyrazinyl, pyrimidinyl, pyridazinyl, pyrrolyl, quinazolinyl, quinoxalinyl, quinolinyl, isoquinolinyl, tetrahydroquinolinyl, 5,6,7,8-tetrahydroquinazolinyl, 5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidinyl, 6,7,8,9-tetrahydro-5H-cyclohepta[4,5]thieno[2,3-d]pyrimidinyl, 5,6,7,8-tetrahydropyrido[4,5-c]pyridazinyl, thiazolyl, thiadiazolyl, thiapyranyl, triazolyl, tetrazolyl, triazinyl, thieno[2,3-d]pyrimidinyl, thieno[3,2-d]pyrimidinyl, thieno[2,3-c]pyridinyl, and thiophenyl (i.e. thienyl). Unless stated otherwise specifically in the specification, a heteroaryl moiety is optionally substituted by one or more substituents which are independently: alkyl, acylsulfonamido, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, nitro, oxo, thioxo, trimethylsilanyl, —OR a , —SR a , —S(O)R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O) t OR (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     Substituted heteroaryl also includes ring systems substituted with one or more oxide (—O—) substituents, such as, for example, pyridinyl N-oxides. 
     “Heteroarylalkyl” refers to a moiety having an aryl moiety, as described herein, connected to an alkylene moiety, as described herein, wherein the connection to the remainder of the molecule is through the alkylene group. 
     “Heterocycloalkyl” refers to a stable 3- to 18-membered non-aromatic ring radical that comprises two to twelve carbon atoms and from one to six heteroatoms selected from nitrogen, oxygen and sulfur. Whenever it appears herein, a numerical range such as “3 to 18” refers to each integer in the given range—e.g., “3 to 18 ring atoms” means that the heterocycloalkyl group may consist of 3 ring atoms, 4 ring atoms, etc., up to and including 18 ring atoms. Unless stated otherwise specifically in the specification, the heterocycloalkyl radical is a monocyclic, bicyclic, tricyclic or tetracyclic ring system, which may include fused or bridged ring systems. The heteroatoms in the heterocycloalkyl radical may be optionally oxidized. One or more nitrogen atoms, if present, are optionally quaternized. The heterocycloalkyl radical is partially or fully saturated. The heterocycloalkyl may be attached to the rest of the molecule through any atom of the ring(s). Examples of such heterocycloalkyl radicals include, but are not limited to, dioxolanyl, thienyl[1,3]dithianyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, octahydroindolyl, octahydroisoindolyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, oxazolidinyl, piperidinyl, piperazinyl, 4-piperidonyl, pyrrolidinyl, pyrazolidinyl, quinuclidinyl, thiazolidinyl, tetrahydrofuryl, trithianyl, tetrahydropyranyl, thiomorpholinyl, thiamorpholinyl, 1-oxo-thiomorpholinyl, and 1,1-dioxo-thiomorpholinyl. Unless stated otherwise specifically in the specification, a heterocycloalkyl moiety is optionally substituted by one or more substituents which independently are: alkyl, acylsulfonamido, heteroalkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, hydroxamate, aryl, arylalkyl, heteroaryl, heteroarylalkyl, hydroxy, halo, cyano, nitro, oxo, thioxo, trimethylsilanyl, —OR a , —SR a , —S(O)R a — (where t is 1 or 2), —OC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O) t R a  (where t is 1 or 2), —S(O) t R a  (where t is 1 or 2), —S(O)ORV (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), or PO(OR a ) 2 , where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Heterocycloalkyl” also includes bicyclic ring systems wherein one non-aromatic ring, usually with 3 to 7 ring atoms, contains at least 2 carbon atoms in addition to 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen, as well as combinations comprising at least one of the foregoing heteroatoms; and the other ring, usually with 3 to 7 ring atoms, optionally contains 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen and is not aromatic. 
     “Hydroxamate” refers to the —C(O)NR a OR a  moiety, where each R a  is independently hydrogen, alkyl, fluoroalkyl, carbocyclyl, carbocyclylalkyl, aryl, aralkyl, heterocycloalkyl, heterocycloalkylalkyl, heteroaryl or heteroarylalkyl. 
     “Nitro” refers to the —NO 2  radical. 
     “Oxa” refers to the —O— radical. 
     “Oxo” refers to the ═O radical. 
     “Isomers” are different compounds that have the same molecular formula. “Stereoisomers” are isomers that differ only in the way the atoms are arranged in space—i.e., having a different stereochemical configuration. “Enantiomers” are a pair of stereoisomers that are non-superimposable mirror images of each other. A 1:1 mixture of a pair of enantiomers is a “racemic” mixture. The term “(f)” is used to designate a racemic mixture where appropriate. “Diastereoisomers” are stereoisomers that have at least two asymmetric atoms, but which are not mirror-images of each other. The absolute stereochemistry is specified according to the Cahn-Ingold-Prelog R-S system. When a compound is a pure enantiomer the stereochemistry at each chiral carbon can be specified by either (R) or (S). Resolved compounds whose absolute configuration is unknown can be designated (+) or (−) depending on the direction (dextro- or levorotatory) which they rotate plane polarized light at the wavelength of the sodium D line. Certain of the compounds described herein contain one or more asymmetric centers and can thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that can be defined, in terms of absolute stereochemistry, as (R) or (S). The present chemical entities, pharmaceutical compositions and methods are meant to include all such possible isomers, including racemic mixtures, optically pure forms and intermediate mixtures. Optically active (R)- and (S)-isomers can be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques. When the compounds described herein contain olefinic double bonds or other centers of geometric asymmetry, and unless specified otherwise, it is intended that the compounds include both E and Z geometric isomers. 
     “Enantiomeric purity” as used herein refers to the relative amounts, expressed as a percentage, of the presence of a specific enantiomer relative to the other enantiomer. For example, if a compound, which may potentially have an (R)- or an (S)-isomeric configuration, is present as a racemic mixture, the enantiomeric purity is about 50% with respect to either the (R)- or (S)-isomer. If that compound has one isomeric form predominant over the other, for example, 80% (S)-isomer and 20% (R)-isomer, the enantiomeric purity of the compound with respect to the (S)-isomeric form is 80%. The enantiomeric purity of a compound can be determined in a number of ways known in the art, including but not limited to chromatography using a chiral support, polarimetric measurement of the rotation of polarized light, nuclear magnetic resonance spectroscopy using chiral shift reagents which include but are not limited to lanthanide containing chiral complexes or Pirkle&#39;s reagents, or derivatization of a compounds using a chiral compound such as Mosher&#39;s acid followed by chromatography or nuclear magnetic resonance spectroscopy. 
     In some embodiments, the enantiomerically enriched composition has a higher potency with respect to therapeutic utility per unit mass than does the racemic mixture of that composition. Enantiomers can be isolated from mixtures by methods known to those skilled in the art, including chiral high pressure liquid chromatography (HPLC) and the formation and crystallization of chiral salts; or preferred enantiomers can be prepared by asymmetric syntheses. See, for example, Jacques, et al.,  Enantiomers. Racemates and Resolutions , Wiley Interscience, New York (1981); E. L. Eliel,  Stereochemistry of Carbon Compounds , McGraw-Hill, New York (1962); and E. L. Eliel and S. H. Wilen,  Stereochemistry of Organic Compounds , Wiley-Interscience, New York (1994). 
     The terms “enantiomerically enriched” and “non-racemic,” as used herein, refer to compositions in which the percent by weight of one enantiomer is greater than the amount of that one enantiomer in a control mixture of the racemic composition (e.g., greater than 1:1 by weight). For example, an enantiomerically enriched preparation of the (S)-enantiomer, means a preparation of the compound having greater than 50% by weight of the (S)-enantiomer relative to the (R)-enantiomer, such as at least 75% by weight, or such as at least 80% by weight. In some embodiments, the enrichment can be significantly greater than 80% by weight, providing a “substantially enantiomerically enriched” or a “substantially non-racemic” preparation, which refers to preparations of compositions which have at least 85% by weight of one enantiomer relative to other enantiomer, such as at least 90% by weight, or such as at least 95% by weight. The terms “enantiomerically pure” or “substantially enantiomerically pure” refers to a composition that comprises at least 98% of a single enantiomer and less than 2% of the opposite enantiomer. 
     “Moiety” refers to a specific segment or functional group of a molecule. Chemical moieties are often recognized chemical entities embedded in or appended to a molecule. 
     “Tautomers” are structurally distinct isomers that interconvert by tautomerization. “Tautomerization” is a form of isomerization and includes prototropic or proton-shift tautomerization, which is considered a subset of acid-base chemistry. “Prototropic tautomerization” or “proton-shift tautomerization” involves the migration of a proton accompanied by changes in bond order, often the interchange of a single bond with an adjacent double bond. Where tautomerization is possible (e.g., in solution), a chemical equilibrium of tautomers can be reached. An example of tautomerization is keto-enol tautomerization. A specific example of keto-enol tautomerization is the interconversion of pentane-2,4-dione and 4-hydroxypent-3-en-2-one tautomers. Another example of tautomerization is phenol-keto tautomerization. A specific example of phenol-keto tautomerization is the interconversion of pyridin-4-ol and pyridin-4(1H)-one tautomers. 
     A “leaving group or atom” is any group or atom that will, under selected reaction conditions, cleave from the starting material, thus promoting reaction at a specified site. Examples of such groups, unless otherwise specified, include halogen atoms and mesyloxy, p-nitrobenzensulphonyloxy and tosyloxy groups. 
     “Protecting group” is intended to mean a group that selectively blocks one or more reactive sites in a multifunctional compound such that a chemical reaction can be carried out selectively on another unprotected reactive site and the group can then be readily removed or deprotected after the selective reaction is complete. A variety of protecting groups are disclosed, for example, in T. H. Greene and P. G. M. Wuts,  Protective Groups in Organic Synthesis,  3rd Edition, John Wiley &amp; Sons, New York (1999). 
     “Solvate” refers to a compound in physical association with one or more molecules of a pharmaceutically acceptable solvent. 
     “Substituted” means that the referenced group may have attached one or more additional groups, radicals or moieties individually and independently selected from, for example, acyl, alkyl, alkylaryl, cycloalkyl, aralkyl, aryl, carbohydrate, carbonate, heteroaryl, heterocycloalkyl, hydroxamate, hydroxy, alkoxy, aryloxy, mercapto, alkylthio, arylthio, cyano, halo, carbonyl, ester, thiocarbonyl, isocyanato, thiocyanato, isothiocyanato, nitro, oxo, perhaloalkyl, perfluoroalkyl, phosphate, silyl, sulfinyl, sulfonyl, sulfonamidyl, sulfoxyl, sulfonate, urea, and amino, including mono- and di-substituted amino groups, and protected derivatives thereof. The substituents themselves may be substituted, for example, a cycloalkyl substituent may itself have a halide substituent at one or more of its ring carbons. The term “optionally substituted” means optional substitution with the specified groups, radicals or moieties. 
     “Sulfanyl” refers to groups that include —S-(optionally substituted alkyl), —S-(optionally substituted aryl), —S-(optionally substituted heteroaryl) and —S-(optionally substituted heterocycloalkyl). 
     “Sulfinyl” refers to groups that include —S(O)—H, —S(O)-(optionally substituted alkyl), —S(O)-(optionally substituted amino), —S(O)-(optionally substituted aryl), —S(O)-(optionally substituted heteroaryl) and —S(O)-(optionally substituted heterocycloalkyl). 
     “Sulfonyl” refers to groups that include —S(O 2 )—H, —S(O 2 )-(optionally substituted alkyl), —S(O 2 )-(optionally substituted amino), —S(O 2 )-(optionally substituted aryl), —S(O 2 )-(optionally substituted heteroaryl), and —S(O 2 )-(optionally substituted heterocycloalkyl). 
     “Sulfonamidyl” or “sulfonamido” refers to a —S(═O) 2 —NRR radical, where each R is selected independently from the group consisting of hydrogen, alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon). The R groups in —NRR of the —S(═O) 2 —NRR radical may be taken together with the nitrogen to which it is attached to form a 4-, 5-, 6- or 7-membered ring. A sulfonamido group is optionally substituted by one or more of the substituents described for alkyl, cycloalkyl, aryl, heteroaryl, respectively. 
     “Sulfoxyl” refers to a —S(═O) 2 OH radical. 
     “Sulfonate” refers to a —S(═O) 2 —OR radical, where R is selected from the group consisting of alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon). A sulfonate group is optionally substituted on R by one or more of the substituents described for alkyl, cycloalkyl, aryl, heteroaryl, respectively. 
     Compounds of the disclosure also include crystalline and amorphous forms of those compounds, including, for example, polymorphs, pseudopolymorphs, solvates, hydrates, unsolvated polymorphs (including anhydrates), conformational polymorphs, and amorphous forms of the compounds, as well as mixtures thereof. “Crystalline form” and “polymorph” are intended to include all crystalline and amorphous forms of the compound, including, for example, polymorphs, pseudopolymorphs, solvates, hydrates, unsolvated polymorphs (including anhydrates), conformational polymorphs, and amorphous forms, as well as mixtures thereof, unless a particular crystalline or amorphous form is referred to. 
     p38 Mitogen-Activated Protein Kinases (MAPKs), Inhibition Thereof, and p38α Selective Inhibition 
     The p38 mitogen-activated protein kinase (MAPKs) family of stress- and cytokine-activated kinases contribute to the pathogenesis of many human diseases, including cancer, rheumatoid arthritis, cardiovascular disease, multiple sclerosis, inflammatory bowel disease, chronic obstructive pulmonary disease (COPD), asthma, acute respiratory distress syndrome (ARDS), and acute lung injury (ALI). Among the many important biological processes regulated by p38 MAPKs, regulation of endothelial and epithelial barrier function, leukocyte trafficking, and cytokine expression are central to the pathogenesis of acute and chronic inflammatory disorders. While preclinical studies support pharmacologic inhibition of p38 as promising treatment for inflammatory diseases, p38 inhibitors have had very limited success in clinical testing because of dose-limiting toxicity and lack of efficacy. Of the 36 Phase II clinical trials of p38 inhibitors listed in www.clinicaltrials.gov, results of only 8 studies have been published or listed in ClinicalTrials.gov and showed little clinical benefit and moderate toxicity. 
     All available p38 inhibitors block catalytic activity either by directly competing for ATP binding or by allosterically causing conformational changes that preclude access of ATP to the catalytic site. Davidson et al. identified a purported p38α substrate-selective inhibitor, CMPD1, which selectively inhibited MK2 phosphorylation in in vitro kinase assays, but it bound near the p38α active site and was subsequently shown to lack substrate-selectivity in cells. Almost all available inhibitors are active against both p38α and p38ß, and some are active against additional isoforms. Yet genetic and pharmacologic studies have identified p38α as the proinflammatory isoform, while other studies have demonstrated p38β signaling to be cytoprotective. Therefore, inhibition of p38β may contribute to both lack of efficacy and toxicity of non-isoform-selective p38 inhibitors. However, the extensive structural conservation of the catalytic module across most protein kinases presents a challenge to developing catalytic inhibitors with high selectivity, especially for individual p38 isoforms. 
     Without wishing to be bound by any particular theory, it is believed that even if the catalytic inhibitors were absolutely selective for p38α, these compounds by design would block all p38α signaling events, many of which are essential for reestablishing and maintaining homeostasis. For example, p38α not only activates expression of proinflammatory cytokines, it also activates anti-inflammatory cytokines and counterregulatory dual specificity protein phosphatase-2 (DUSP2) through the p38α substrate, MSK1/2. The transient decrease and subsequent rebound of serum C-reactive protein (CRP) levels seen in clinical trials of p38 catalytic inhibitors might be caused by loss of the MSK1/2-dependent anti-inflammatory signaling. 
     As an alternative to the catalytic inhibitors, in some embodiments, the compounds and methods of the disclosure target the substrate binding groove of p38α, which stretches between two acidic patches, the CD and ED domains, and is distinct from the DEF substrate-binding pocket. Downstream substrates, upstream activating kinases, and possibly scaffolding molecules, all interact with p38 through these sites. In some embodiments, computer-aided drug design (CADD) and/or rational design were used in order to target low molecular weight compounds to a pocket near the p38α ED substrate binding site, which is required for phosphorylation of MAPK-activated protein kinase-2 (MAPKAPK2; MK2), a p38α substrate known to mediate endothelial permeability and neutrophil transendothelial migration (TEM) in vitro, and pulmonary edema in a mouse lung injury model; whereas anti-inflammatory MSK1/2 binds to the CD site. Using this algorithm, p38α-binding compounds with high efficiency were identified, compounds able to: stabilize endothelial barrier function in human lung microvascular endothelial cells (HMVECLs), inhibit LPS-induced proinflammatory gene expression in THP1 cells, and be well tolerated and more potent than SB203580 in mitigating experimental ALI. 
     Acute Lung Injury Treated by the p38α MAPK Inhibitors and Methods Described Herein 
     Acute respiratory distress syndrome (ARDS) is a common cause of respiratory failure, which has 30-40% mortality and no effective therapeutic options. p38 signaling is important in ARDS pathogenesis, but clinical trials of p38 inhibitors, all of which inactivate the p38 catalytic site and block phosphorylation of all p38 substrates, have been disappointing due to dose-limiting toxicity. 
     Acute respiratory distress syndrome (ARDS) is characterized by acute onset of non-hydrostatic pulmonary edema caused predominantly by neutrophil-mediated injury to the alveolar epithelium and capillary endothelial barrier dysfunction. A complex network of pro- and anti-inflammatory mediators activated in association with ARDS is critical to the pathogenesis of acute lung injury (ALI) as well as the multiple organ failure that often accompanies ARDS. However, therapeutic agents that target proinflammatory mediators have proven to be ineffective in ARDS. Injury to lung parenchyma causes reduced compliance, intrapulmonary shunting, and mismatched ventilation-perfusion that usually necessitates mechanical ventilation. However, the cyclical recruitment/de-recruitment of alveoli and overdistension caused by mechanical ventilation can itself cause neutrophil-dependent inflammation and lung injury even to previously normal lungs. Appreciation of this mechanism led to a Phase III randomized clinical trial demonstrating that mechanical ventilation with low tidal volumes improves survival in patients with ARDS. Two additional supportive maneuvers have been shown to improve mortality in patients with severe ARDS, neuromuscular blockade and prone positioning. A third intervention, conservative fluid management, was shown to decrease duration of mechanical ventilation and ICU length of stay, but not mortality. Despite these improvements in supportive care, mortality in patients with ARDS remains 30-40% with about 74,500 deaths per year in the United States, underscoring the importance of developing new therapeutics that target the relevant pathogenic mechanisms. 
     The p38 mitogen-activated protein kinases (MAPKs) are a family of stress- and cytokine-activated kinases that are activated by many of the pathogenic signals associated with ARDS, including inflammatory mediators, febrile-range hyperthermia (FRH), and cyclic stretch. Since p38 MAPK is activated in patients at-risk for ARDS and, as discussed below, p38 MAPK participates in multiple processes that contribute to the pathogenesis of ARDS, this family of MAPKs presents an intriguing therapeutic target in ARDS. As proof of this concept, the prototypical pyridinyl imidazole compound SB203580, which inhibits kinase activity of p38α and β, but not p38γ or δ has been shown to block multiple processes that contribute to the pathogenesis of ARDS. Endothelial p38 signaling is activated by neutrophil binding and required for neutrophil transendothelial migration (TEM). Cross-linking ICAM-1 on human umbilical vein endothelial cells (HUVECs) stimulates p38α activation, HSP27 phosphorylation, F-actin rearrangement, ICAM-1 aggregation, and cell stiffening, and increases migration of neutrophils to HUVEC intercellular junctions, all of which is blocked by SB203580. Cross-linking E-selectin on HUVECs activates p38 and p38-dependent cytoskeleton rearrangement, stress fiber formation and neutrophil TEM. Similarly, ICAM-1 ligation of B2 integrins on neutrophils activates neutrophil p38 and p38-dependent chemokinesis and chemotaxis. Pretreatment with p38 inhibitor was protective in a mouse model of ventilator-induced lung injury, complement-induced lung injury, and lung injury associated with a cecal-ligation and puncture model of sepsis, but not hemorrhage and endotoxemia-induced lung injury. It was shown that the exaggerated endothelial barrier dysfunction caused by FRH (2-3° C. increase in core temperature) in experimental ALI is associated with p38 activation and blocked by SB203580. The effectiveness of SB203580 in mitigating multiple pathogenic processes that contribute to acute lung injury and the relatively high expression of p38α and β in human lung supports a central role for these two p38 isoforms in the pathogenesis of ARDS. 
     Despite these persuasive preclinical data there has been only one clinical trial that begins to evaluate p38 inhibition as a therapeutic strategy in ARDS. This early phase Ha trial of SB-681323/dilmapimod in patients at-risk for ARDS (clinicaltrials.gov no. NCT00996840) showed dilmapimod was safe at the doses administered and modestly reduced serum C-reactive protein (CRP) levels, but was not powered to analyze effects on ARDS incidence or severity. There are currently a total of 74 clinical trials of p38 inhibitors listed in www.clinicaltrials.gov, including 26 Phase I, 47 Phase II, and one Phase III trial. Phase II and III trials tested safety and efficacy of ten different p38 catalytic inhibitors for 13 different disease/indications including analgesia (6 trials), osteoarthritis (2 trials), rheumatoid arthritis (13 trials), Alzheimer&#39;s disease (2 trials), ankylosing spondylitis (1 trial), cardiomyopathy (1 trial), psoriasis (2 trials), atherosclerosis (5 trials), depression (2 trials), COPD (8 trials), at-risk ARDS (1 trial), cancer (4 trials), and glomerulosclerosis (1 trial). Although only a portion of the data is in the public domain, the failure of most of these drugs seems to have been due to adverse side-effect profiles or lack of effectiveness at the doses used. Of the 48 Phase II and III trials 36 have been completed and 3 terminated early, but results of only 8 studies have been published or listed in ClinicalTrials.gov. Two trials of VX-702 in rheumatoid arthritis showed small increases in proportion of treated subjects with ACR20 symptom score vs. placebo. Of two published studies of p38 inhibitors for pain, one reported modest reduction in pain and the other no effect. Of two published studies of p38 inhibitors in COPD, one showed no effect and the other showed a 100 ml increase in FEV1 and a decrease in serum CRP levels in the treatment group, but with associated toxicity (rash, pharyngitis, prolonged QTc). GW85655 (losmapimod), improved vascular relaxation and reduced serum CRP in patients with hypercholesterolemia. In a ninth clinical trial, which was not listed in clinicaltrials.gov, BIRB 796 (doramapimod) had no clinical effect in patients with Crohn&#39;s disease but transiently reduced serum CRP levels. Collectively, these studies demonstrate the therapeutic potential of p38 inhibition in a broad range of human disease, but underscore the limited efficacy of the currently available p38 inhibitors at doses that can be safely administered to humans. 
     The p38 MAPKs, like most protein kinases, share a conserved bi-lobed structure and a catalytic site, with its hydrophobic ATP-binding pocket, located between the N-terminal and C-terminal lobes. Most available protein kinase inhibitors compete with ATP for binding to the ATP-binding pocket of the catalytic site, but the extensive structural conservation of the catalytic module across most protein kinases presents a challenge to developing catalytic p38 inhibitors with high specificity. Since the pyridinyl imidazole inhibitor SB203580 binds the ATP-binding site of p38α and β, but its access to the ATP binding site of p38γ and δ is blocked by a bulky methionine, it is used as a specific inhibitor of p38α and β. However, proteomic analysis identified several additional kinases that were inhibited by SB203580 with sub-micromolar IC 50 , including Rip-like interacting caspase-like apoptosis-regulatory protein kinase (RICK/Rip2), casein kinase (CK)-1δ, and cyclin G-associated kinase (GAK). 
     A new class of diaryl urea compounds was discovered in a high throughput biochemical screen for p38 inhibitors. Rather than bind directly to the ATP binding pocket, these compounds bind to an allosteric site that induces a conformational change in p38 that precludes access of ATP to its binding pocket in the catalytic site. Three allosteric p38 inhibitors, BIRB 796/dormapimod, GW856553/losmapimod, and SB-681323/dilmapimod have entered clinical testing, but like the ATP-competitive, have not progressed beyond phase II testing except for the LATITUDE study, an ongoing Phase III trial of losmapimod in patients with acute coronary syndrome (clinicaltrials.gov no. NCT02145468). Since the allosteric inhibitors are not affected by the presence of the gatekeeper methionine, these compounds inhibit all four p38 isoforms, but BIRB 796 also potently inhibits Jnk2α2 with IC 50  of 0.1 μM and c-Raf-1 with IC 50  1.4 μM. The lack of specificity of the ATP-competitive and allosteric p38 inhibitors is likely a major source of off-target toxicity. 
     An equally important source of p38 inhibitor toxicity likely derives from the broad range of functions of each p38 MAPK isoform. Since both types of inhibitors block the p38 catalytic site, the ATP-competitive and allosteric inhibitors block all p38 phosphorylation events. Since p38 phosphorylates at least 66 recognized substrates with important biological activity, dose-limiting toxicity may be unavoidable with these agents. 
     MAPK p38 and ERK family members share a structural feature, a substrate binding groove located on the C-terminal lobe of the protein on the side opposite the catalytic domain. The binding groove stretches between two acidic patches, the CD and ED domains. This region of p38 not only binds p38 substrates but also binds upstream kinases and scaffolding proteins. Our group has previously developed a new class of ERK1/2 MAPK inhibitors with improved toxicity profile by using computer-aided drug design (CADD) to identify small molecules that target the substrate binding groove rather than the catalytic module of ERK2. As described herein, a similar strategy may be employed to identify low molecular weight compounds targeting a pocket near the p38α ED substrate binding site, which is required for phosphorylation of MK2, a p38 substrate known to mediate pulmonary endothelial permeability in vitro and pulmonary edema in a mouse lung injury model. Using CADD to search a database of commercially available compounds, 150 low molecular weight compounds predicted to bind to the targeted pocket near the ED binding site of p38α target have been identified. Twenty structurally distinct compounds from this list were obtained, screened for selective binding to p38α but not ERK2 by differential scanning fluorimetry (DSF), then analyzed for capacity to reduce pathogenic endothelial barrier changes in human lung microvascular endothelial cells (HMVECLs) and cytokine expression in THP1 monocytes in vitro, and to mitigate ALI induced in mice. Of the 20 CADD-selected compounds tested, five bound to p38α with sufficient affinity to detect by DSF, two bound selectively to p38α but not ERK2 and were more effective than SB203580 in stabilizing endothelial barrier function in vitro, and one of these compounds was well tolerated and more potent than SB203580 in mitigating experimental ALI. 
     In certain embodiments, the p38α MAPK inhibitors described herein may be used in the treatment of acute respiratory distress syndrome (ARDS) and/or acute lung injury (ALI). 
     p38α MAPK Inhibitors and Methods of Inhibiting p38α MAPK 
     In an embodiment, the disclosure includes compounds that may be p38α MAPK inhibitors and/or modulators of p38α MAPK protein activity, for example compounds capable of binding to a pocket near the ED substrate-docking site of p38α MAPK, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof. In one embodiment, the p38α MAPK inhibitor is a p38α MAPK selective inhibitor. In an embodiment, the p38α MAPK inhibitor binds p38α MAPK near the substrate binding groove of p38α MAPK, which stretches between two acidic patches, the CD and ED domains. In other embodiments, the p38α MAPK inhibitor causes inhibition of MK2 phosphorylation. 
     In one embodiment, a lead p38α MAPK inhibitor compound has been identified, that has favorable biological effects in human cell culture models and in a mouse model of inflammatory lung injury. In one embodiment, a p38α MAPK inhibitor has been identified by means of a CADD strategy. The CADD-targeted pocket in p38α differed from the corresponding pocket in p38β in 3 of 10 amino acids, which provided an opportunity for p38α-selectivity. In some embodiments, the sequence of the targeted pocket at least includes amino acids R49, H107, L108, and K165 in p38α MAPK. In some embodiments, the sequence of the targeted pocket is R49, H107, L108, M109, G110, A157, V158, E163, L164, and K165 in p38α MAPK. In one embodiment, a p38α MAPK inhibitor has been identified by means of a rational design strategy. 
     In some embodiments, the p38α MAPK inhibitor is a compound is a compound of Formula A, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof: 
     
       
         
         
             
             
         
       
     
     wherein in Formula A: Ar and Ar 1  are independently selected from a mono- or polycyclic optionally substituted cycloalkyl, mono- or polycyclic optionally substituted heterocycloalkyl, mono- or polycyclic optionally substituted aryl, mono- or polycyclic optionally substituted arylalkyl, mono- or polycyclic optionally substituted heteroaryl, and mono- or polycyclic optionally substituted heteroarylalkyl; R 1  and R 2  are independently selected from hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted alkylheteroaryl, wherein R 1  and R 2  can optionally be joined to form a carbo- or heterocycle; L 1  and L 2  are linkers comprising independently one or more of a bond, —NR a —, —S—, —S(O)—, —S(O) 2 —, —O—, —CR a   2 —, —C(O)O—, —OC(O)—, —C(O)S—, —SC(O)—, —C(O)NR a —, —NR a C(O)—, —C(O)NR a SO 2 —, —SO 2 NR a  C(O)—, —OC(O)O—, —OC(O)S—, —SC(O)O—, —OC(O)NR a —, —NR a C(O)O—, —S(O) t N(R a )— (where t is 1 or 2), —N(R a )S(O)— (where t is 1 or 2), disubstituted alkyl, disubstituted heteroalkyl, disubstituted alkenyl, disubstituted alkynyl, disubstituted cycloalkyl, disubstituted heterocycloalkyl, disubstituted aryl, disubstituted arylalkyl, disubstituted heteroaryl, and disubstituted heteroarylalkyl; wherein any optional substituent is independently selected at each occurrence from optionally substituted alkyl, optionally substituted heteroalkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted arylalkyl, optionally substituted heteroaryl, optionally substituted heteroarylalkyl, hydroxy, halo, cyano, trifluoromethyl, trifluoromethoxy, nitro, trimethylsilanyl, —OR a , —SR a , —OC(O)—R a , —SC(O)—R a , —N(R a ) 2 , —C(O)R a , —C(O)OR a , —C(O)SR a , —OC(O)N(R a ) 2 , —C(O)N(R a ) 2 , —N(R a )C(O)OR a , —N(R a )C(O)R a , —N(R a )C(O)N(R a ) 2 , —N(R a )C(NR a )N(R a ) 2 , —N(R a )S(O)R a  (where t is 1 or 2), —S(O)R a  (where t is 1 or 2), —S(O) t OR a  (where t is 1 or 2), —S(O) t N(R a ) 2  (where t is 1 or 2), and PO 3 (R a ) 2 ; and R a  is independently selected at each occurrence from hydrogen, optionally substituted alkyl, optionally substituted fluoroalkyl, optionally substituted carbocyclyl, optionally substituted carbocyclylalkyl, optionally substituted aryl, optionally substituted aralkyl, optionally substituted heterocycloalkyl, optionally substituted heterocycloalkylalkyl, optionally substituted heteroaryl, and optionally substituted heteroarylalkyl. In some embodiments, an optional substituent is independently selected from R 3 , R 4 , R 5 , and R 6 , which are independently selected from hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted alkylheteroaryl, wherein any two of R 1 , R 2 , R 3 , R 4 , R 5 , and R 6  can optionally be joined to form a carbo- or heterocycle. In some embodiments, Ar 1  is an optionally substituted aryl or optionally substituted heteroaryl ring. In some embodiments, Ar is a mono- or polycyclic optionally substituted aryl, a mono- or polycyclic optionally substituted arylalkyl, a mono- or polycyclic optionally substituted heteroaryl, or a mono- or polycyclic optionally substituted heteroarylalkyl. In some embodiments, Ar is a 5 or 6 membered optionally substituted aryl or a 5 or 6 membered optionally substituted heteroaryl, wherein the L 1  and L 2  are connected to Ar in a 1,2, 1,3, or 1,4 substitution pattern. In some embodiments, Ar is 1,2 disubstituted, 1,3 disubstituted, or 1,4 disubstituted phenyl, pyridine, pyrimidine, pyrazine, or triazine. In some embodiments, Ar is 1,2 disubstituted or 1,3 disubstituted furan, thiophene, pyrrole, thiazole, imidazole, oxazole, triazole, or pyrazole. In some embodiments, Ar is 1,2 disubstituted, 1,3 disubstituted, 1,4 disubstituted, 1,5 disubstituted, 1,6 disubstituted, 1,7 disubstituted, or 1,8 disubstituted naphthalene, quinoline, isoquinoline, or quinazoline. In some embodiments, Ar is 1,2 disubstituted, 1,3 disubstituted, 1,4 disubstituted, 1,5 disubstituted, 1,6 disubstituted, or 1,7 disubstituted indole or imidazole. In some embodiments, L 1  is a linker selected from —CH 2 —, —C(CH 3 ) 2 —, and —C(CH 2 CH 2 )—; L 2  is a linker selected from —NHCH 2 —, —CH 2 NH—, —NHCO—, —CONH—, —SO 2 NH—, and —NHSO 2 —; and —NR 1 R 2  is selected from: 
     
       
         
         
             
             
         
       
     
     wherein R 7  is hydrogen or an optionally substituted alkyl, and Ar 2  is a heterocycle. 
     In one embodiment, the p38α MAPK inhibitor is a compound of Formula I, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof: 
     
       
         
         
             
             
         
       
     
     wherein in Formula I: each of R 1 , R 2 , R 3 , R 4 , R 5 , and R 6  is independently selected from hydrogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted alkylheteroaryl, wherein R 1  and R 2  can optionally be joined to form a carbo- or heterocycle; L 1  is a linker selected from —CH 2 —, —C(CH 3 ) 2 —, and —C(CH 2 CH 2 )—; L 2  is a linker selected from —NHCH 2 —, —CH 2 NH—, —NHCO—, —CONH—, —SO 2 NH—, and —NHSO 2 —; and Ar 1  is an optionally substituted aryl or optionally substituted heteroaryl ring. In some embodiments, the —NR 1 R 2  group in Formula I is selected from: 
     
       
         
         
             
             
         
       
     
     wherein R 7  is hydrogen or an optionally substituted alkyl, and Ar 2  is a heterocycle. In some embodiments, the —NR 1 R 2  group in Formula I is selected from: 
     
       
         
         
             
             
         
       
     
     In some embodiments, L 1  is —CH 2 —. In some embodiments, R 3 , R 4 , R 5 , and R 6  are hydrogens. In some embodiments, L 2  is selected from —NHCH 2 —, —NHCO—, and —NHSO 2 —. In some embodiments, Ar 1  is selected from: 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     wherein each of R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , and R 13  is independently selected from hydrogen, halogen, —NR 1 R 2 , alkoxy, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted alkoxy, optionally substituted aryl, optionally substituted heteroaryl, and optionally substituted alkylheteroaryl, and wherein any two vicinal of R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , and R 13  can optionally be joined to form a carbo- or heterocycle. In some embodiments, Ar 1  is selected from: 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     In one embodiment, the p38α MAPK inhibitor is a compound of Formula II, and can be selected from any one compound of Formulas 1001-1180 in Table 1: 
     
       
         
         
             
             
         
       
     
     
       
         
           
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Compound # 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 L 2   
                 Ar 1   
               
               
                   
               
             
            
               
                 1001 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1002 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1003 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1004 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1005 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1006 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1007 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1008 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1009 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1010 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1011 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1012 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1013 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1014 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1015 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1016 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1017 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1018 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1019 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1020 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1021 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1022 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1023 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1024 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1025 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1026 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1027 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1028 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1029 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1030 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCH 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1031 (UM101) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1032 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1033 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1034 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1035 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1036 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1037 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1038 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1039 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1040 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1041 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1042 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1043 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1044 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1045 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1046 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1047 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1048 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1049 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1050 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1051 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1052 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1053 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1054 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1055 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1056 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1057 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1058 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1059 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1060 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHCO— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1061 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1062 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1063 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1064 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1065 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1066 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1067 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1068 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1069 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1070 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1071 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1072 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1073 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1074 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1075 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1076 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1077 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1078 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1079 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1080 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1081 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1082 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1083 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1084 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1085 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1086 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1087 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1088 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1089 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1090 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —NHSO 2 — 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1091 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1092 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1093 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1094 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1095 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1096 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1097 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1098 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1099 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1100 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1101 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1102 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1103 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1104 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1105 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1106 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1107 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1108 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1109 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1110 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1111 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1112 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1113 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1114 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1115 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1116 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1117 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1118 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1119 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1120 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CH 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1121 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1122 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1123 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1124 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1125 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1126 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1127 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1128 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1129 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1130 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1131 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1132 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1133 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1134 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1135 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1136 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1137 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1138 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1139 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1140 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1141 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1142 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1143 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1144 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1145 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1146 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1147 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1148 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1149 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1150 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —CONH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1151 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1152 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1153 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1154 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1155 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1156 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1157 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1158 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1159 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1160 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1161 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1162 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1163 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1164 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1165 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1166 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1167 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1168 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1169 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1170 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1171 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1172 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1173 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1174 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1175 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1176 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1177 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1178 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1179 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1180 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —SO 2 NH— 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     In one embodiment, the p38α MAPK inhibitor is a compound of any of Formulas 1001 (SF-6-221), 1032 (SF-7-008), SF-7-009, 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), and 1087 (SF-7-044): 
     
       
         
           
               
               
             
               
                   
               
             
            
               
                 1001 (SF-6-221) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1032 (SF-7-008) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 SF-7-009 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1034 (SF-7-010) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1035 (SF-7-011) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1035-b 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1036 (SF-7-012) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1037 (SF-6-217) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1043 (SF-6-223) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1049 (SF-6-224) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1061 (SF-6-219) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1085 (SF-6-222) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 1087 (SF-7-044) 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
               
                   
               
            
           
         
       
     
     Selective binding of UM101 to p38α was confirmed using complementary technologies. DSF, which detects ligand-induced protein stabilization showed UM101 to cause a concentration-dependent increase in melting temperature of p38α but not p38β ( FIG.  3   d   ). The smaller effect of UM101 compared with SB203580 on p38α melting suggests lower p38α binding affinity of substrate-selective vs. catalytic inhibitors, which is similar substrate-selective ERK inhibitors. The smaller effect of SB203580 on p38β than p38α is consistent with the known ˜10-fold higher binding affinity of SB203580 for p38α. STD-NMR, which measures low affinity protein:ligand binding via non-scalar magnetization transfer from protein to ligand protons, confirmed specific UM101 binding to p38α and localized the interaction to its aromatic rings. UM101 binding to its CADD target was also confirmed by showing that mutating four of ten amino acids in the targeted pocket abrogated UM101 binding while SB203580 binding was preserved. 
     In some embodiments, the p38α MAPK inhibitor causes a concentration-dependent increase in melting temperature of p38α MAPK. The difference in melting temperature ΔTm (° C.) is measured at a p38α MAPK inhibitor concentration of between 1 nM and 1000 μM. In one embodiment, the difference in melting temperature ΔTm (° C.) is measured at a p38α MAPK inhibitor concentration of 100 μM. In one embodiment, ΔTm is between about 0.1 and about 2° C. In one embodiment, ΔTm is between about 0.01 and about 0.05° C. In one embodiment, ΔTm is between about 0.01 and about 0.1° C. In one embodiment, ΔTm is between about 0.03 and about 0.7° C. In one embodiment, ΔTm is between about 0.06 and about 1.5° C. In one embodiment, ΔTm is between about 1° C. and about 2° C. In one embodiment, ΔTm is between about 1.5 and about 2° C. In one embodiment, ΔTm is about 0.1° C. In one embodiment, ΔTm is about 0.2° C. In one embodiment, ΔTm is about 0.3° C. In one embodiment, ΔTm is about 0.4° C. In one embodiment, ΔTm is about 0.5° C. In one embodiment, ΔTm is about 0.6° C. In one embodiment, ΔTm is about 0.7° C. In one embodiment, ΔTm is about 0.8° C. In one embodiment, ΔTm is about 0.9° C. In one embodiment, ΔTm is about 1° C. In one embodiment, ΔTm is about 1.1° C. In one embodiment, ΔTm is about 1.2° C. In one embodiment, ΔTm is about 1.3° C. In one embodiment, ΔTm is about 1.4° C. In one embodiment, ΔTm is about 1.5° C. In one embodiment, ΔTm is about 1.6° C. In one embodiment, ΔTm is about 1.7° C. In one embodiment, ΔTm is about 1.8° C. In one embodiment, ΔTm is about 1.9° C. In one embodiment, ΔTm is about 2° C. In one embodiment, ΔTm is about 0.735° C. In one embodiment, ΔTm is about 0.667° C. 
     In some embodiments, the p38α MAPK inhibitor has a log P between about −5 and about 10. In some embodiments, the p38α MAPK inhibitor has a log P between about −3 and about 8. In some embodiments, the p38α MAPK inhibitor has a log P between about 0 and about 5. In some embodiments, the p38α MAPK inhibitor has a log P between about 0.1 and about 3. log P is a measure of drug solubility, and is defined as the logarithm of the octanol/water partition coefficient of the drug. In one embodiment, the p38α MAPK inhibitor has a log P between about 0.1 and about 1. In one embodiment, the p38α MAPK inhibitor has a log P between about 0.5 and about 1.5. In one embodiment, the p38α MAPK inhibitor has a log P between about 0.75 and about 2. In one embodiment, the p38α MAPK inhibitor has a log P between about 1 and about 2.5. In one embodiment, the p38α MAPK inhibitor has a log P between about 1.75 and about 3. In one embodiment, the p38α MAPK inhibitor has a log P of about 0.1. In one embodiment, the p38α MAPK inhibitor has a log P of about 0.25. In one embodiment, the p38α MAPK inhibitor has a log P of about 0.5. In one embodiment, the p38α MAPK inhibitor has a log P of about 0.75. In one embodiment, the p38α MAPK inhibitor has a log P of about 1. In one embodiment, the p38α MAPK inhibitor has a log P of about 1.25. In one embodiment, the p38α MAPK inhibitor has a log P of about 1.5. In one embodiment, the p38α MAPK inhibitor has a log P of about 1.75. In one embodiment, the p38α MAPK inhibitor has a log P of about 2. In one embodiment, the p38α MAPK inhibitor has a log P of about 2.25. In one embodiment, the p38α MAPK inhibitor has a log P of about 2.5. In one embodiment, the p38α MAPK inhibitor has a log P of about 2.75. In one embodiment, the p38α MAPK inhibitor has a log P of about 3. In one embodiment, the p38α MAPK inhibitor has a log P of about 0.28. In one embodiment, the p38α MAPK inhibitor has a log P of about 2.31. 
     Phosphorylation of MK2 requires binding to the ED site adjacent to the CADD target pocket in p38α MAPK. In some embodiments, the target pocket is at least defined by amino acids R49, H107, L108, and K165 in p38α MAPK. In some embodiments, the target pocket is defined by amino acids selected from the group consisting of R49, H107, L108, M109, G110, A157, V158, E163, L164, and K165 in p38α MAPK, and combinations thereof. In some embodiments, the target pocket is defined by the amino acids R49, H107, L108, M109, G110, A157, V158, E163, L164, and K165 in p38α MAPK. Western blotting confirmed partial inhibition of MK2 phosphorylation in anisomycin-stimulated HeLa cells by UM101, but less compared with 10 μM SB203580. SB203580 at a concentration 200- and 20-fold higher than the IC 50  for p38α and p38β, respectively failed to completely block MK2 phosphorylation, which may reflect a contribution from p38γ or δ as both isoforms are expressed in HeLa cells. 
     In one embodiment, the disclosure relates to a method of inhibiting p38α MAPK where inhibiting p38α MAPK stabilizes an endothelial or epithelial barrier function. Both of the selective p38α binding compounds, UM60 and UM101, exerted SB203580-like endothelial-barrier-stabilizing and macrophage-cytokine-modifying effects, thereby validating the ED-targeting strategy. UM101 more effectively stabilized endothelial barriers than SB203580 ( FIG.  2   a    and  FIG.  2   b   ) despite having less effect on MK2 phosphorylation. In one embodiment, endothelial barrier permeability can be measured by separate or combined exposure to TNFα and hyperthermia, followed by measurement of permeability for 10 kDa dextran. In one embodiment, endothelial barrier stabilization is assessed by pretreating with a compound of the disclosure, preceded and followed by permeability measurements, where stabilization is expressed as a % reduction in the before and after pretreatment permeability increase. Pretreatment with a p38α MAPK inhibitor can be done at various concentrations, for example at 10, 25, 50, or 100 μM. In one embodiment, the permeability increase for 10 kDa dextran can be reduced by between 5% to more than 100%. In one embodiment, the permeability increase is reduced by about 5%. In one embodiment, the permeability increase is reduced by about 10%. In one embodiment, the permeability increase is reduced by about 20%. In one embodiment, the permeability increase is reduced by about 30%. In one embodiment, the permeability increase is reduced by about 40%. In one embodiment, the permeability increase is reduced by about 50%. In one embodiment, the permeability increase is reduced by about 60%. In one embodiment, the permeability increase is reduced by about 70%. In one embodiment, the permeability increase is reduced by about 80%. In one embodiment, the permeability increase is reduced by about 90%. In one embodiment, the permeability increase is reduced by about 100%. In one embodiment, the permeability increase is reduced by about more than 100%. In one embodiment, the permeability increase is reduced by about 71%. In one embodiment, the permeability increase is reduced by about 74%. In one embodiment, the permeability increase is reduced by about 89%. In one embodiment, the permeability increase is reduced by about 100%. 
     Since UM101 more effectively stabilized endothelial barriers than SB203580 ( FIG.  2   a    and  FIG.  2   b   ) despite having less effect on MK2 phosphorylation ( FIG.  3   c   ), additional molecular actions were evaluated by comparing the effects of UM101 and SB203580 on global gene expression using RNASeq in TNFα-treated HMVECLs. TNFα increased expression of 511 genes by ≥2-fold, of which 61 were reduced and 38 increased by pretreatment with 10 μM SB203580. Despite using a concentration of UM101 that was &gt;10-fold higher than required to stabilize HMVECL barrier functions ( FIG.  2   a    and  FIG.  2   b   ), UM101 modified expression of only 38 of the 99 SB203580-modified genes. PathwayNet analysis showed UM101 to block only 7 of the 15 SB203580-blocked transcription factors. MSK1/2 was among those spared by UM101, which is consistent with the targeting strategy for UM101 for the ED site, and in an advantageous way, given the anti-inflammatory actions of MSK1/2. 
     The partial functional overlap of UM101 and SB203580 revealed by RNASeq is consistent with the design of UM101 as a non-catalytic substrate-selective inhibitor, but might also be the result of off-target effects of SB203580, which include Receptor-interacting Protein Kinase-2, cyclin G-associated kinase, and casein kinase-1S. However, none of the SB203580-inhibited transcription factors identified by the PathwayNet analysis are known substrates for these kinases as analyzed using PhosphoNetworks. 
     Although the high concentration of UM101 used in this analysis may have caused some p38-independent actions, the data described herein support a conclusion that UM101 exerts its biological effects predominantly by modifying p38α: (1) DSF and STD-NMR show p38α-specific binding of UM101; (2) p38α binding of UM101 was abrogated by mutating 4 of 10 target pocket amino acids; (3) UM60 and 101 both bind p38α and exert effects on endothelial function similar to SB203580; (4) UM101 partially blocked phosphorylation of the p38 substrates MK2 and Stat-1 in TNFα-stimulated HeLa cells; and (5) UM101 inhibited expression of about half the genes inhibited by SB203580. UM101 may be more effective than SB203580 in stabilizing endothelial barrier because of its selective sparing of potential counter-regulatory genes, such as GM-CSF, MSK1/2-dependent anti-inflammatory genes, and p38ß-dependent pro-survival genes. 
     In one embodiment, the disclosure relates to a method of inhibiting p38α MAPK, including contacting the p38α MAPK with a compound capable of binding to a pocket near the ED substrate-docking site of p38α MAPK, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof. In one embodiment, the compound selectively inhibits p38α MAPK. In an embodiment, the p38α MAPK inhibitor binds p38α MAPK near the substrate binding groove of p38α MAPK, which stretches between two acidic patches, the CD and ED domains. In one embodiment, the binding pocket is defined at least by residues R49, H107, L108, and K165 in p38α MAPK. In one embodiment, the binding pocket is defined by residues R49, H107, L108, M109, G110, A157, V158, E163, L164, and K165 in p38α MAPK. In some embodiments, the p38α MAPK inhibitor causes a concentration-dependent increase in melting temperature of p38α MAPK. In other embodiments, the p38α MAPK inhibitor causes inhibition of MK2 phosphorylation. In one embodiment, the compound is of any of Formulas A, I, II, 1001-1180, in particular of any of Formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044), or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof. 
     In one embodiment, the disclosure relates to a method of inhibiting p38α MAPK where inhibition of p38α MAPK does not result in loss of p38α-dependent counterregulatory responses. In some embodiments, the p38α-dependent counterregulatory response relates to mitogen- and stress-activated protein kinase-1 (MSK1), or MSK2. In targeting a pocket near the ED substrate-docking site of p38α, the inhibitors described herein avoid interfering with CD-specific substrates, including MSK1/2, thus limiting inflammation through expression of IL-10 and DUSP2. Among the effects of MSK1/2 deletion in mice is increased and prolonged LPS-induced expression of the CRP-regulator, IL-6, suggesting a possible mechanism of the rebound in serum CRP observed in some clinical trials of catalytic p38 inhibitors. 
     In one embodiment, the disclosure relates to a method of inhibiting p38α MAPK where inhibiting p38α MAPK reduces inflammation. In one embodiment, the effects of a p38α MAPK inhibitor on inflammatory cytokine expression are compared by pretreating PMA-differentiated THP1 cells with a p38α MAPK inhibitor, then stimulating with LPS, and harvesting RNA a period of time later for analysis by PCR-based cytokine array. In some embodiments, a p38α MAPK inhibitor inhibits expression of various genes, such as IL-1A, IL-8, TNFSF8, CXCL5, CCL7, CCL17, TNFSF9, IL-1B, CXCL1, TNFSF15, CCL5, CCL4, CCL20, CXCL2, TNF, or BMP6. In some embodiments, a p38α MAPK inhibitor inhibits expression of Smad3, which drives differentiation of Foxp3 T regulatory cells and suppresses interferon-gamma. The p38α MAPK inhibitor can be used in any appropriate concentration, for example 10, 25, 50, or 100 μM. In one embodiment, inflammation reduction is measured by comparing the fold change mRNA levels vs. unstimulated PMA-differentiated THP1 cells at various concentrations of p38α MAPK inhibitor. 
     In some embodiments, a p38α MAPK inhibitor modulates TNFα-induced gene expression in HMVECLs, as evidenced using RNASeq. In one embodiment, HMVECLs were pretreated for a period of time with a p38α MAPK inhibitor at an appropriate concentration, for example 10 μM or 100 μM, and then stimulated with TNFα for a period of time. A p38α MAPK inhibitor of the disclosure inhibits genes such as PRRG4, TSLP, CCL17, EXOC3L4, MMP9, IDO1, CXCL10, CD200, SLC15A3, VDR, IL1B, GPR88, CD207, TCHH, HAS3, GBP1P1, MUC4, ELOVL7, CXCL11, GBP4, PLA1A, or CXCL5. 
     In one embodiment, the disclosure relates to a method of inhibiting p38α MAPK where inhibiting p38α MAPK mitigates lung injury in a subject, including, but not limited to, LPS-induced lung injury. In one embodiment, the effectiveness of a p38α MAPK inhibitor in mitigating transalveolar protein and neutrophil extravasation in a mouse model of LPS/hyperthermia-induced ALI was compared ( FIG.  2   c    and  FIG.  2   d   ). In one embodiment, subjects receive intraperitoneal injection(s) of a p38α MAPK inhibitor at concentrations such as 100, 250, 300, 400, 500, 750, 1000 μg, or the like, in an appropriate carrier, for example DMSO, a period of time prior to intratracheal instillation of LPS, and/or transfer to hyperthermic chambers. Lung lavage from subjects are measured for protein and/or neutrophils. Compared with control subjects, lavage protein concentration and neutrophil content in subjects pretreated with a p38α MAPK inhibitor are reduced. In some embodiments, the reduction is between about 5% and about 100%. In one embodiment, the reduction is greater than about 5%. In one embodiment, the reduction is greater than about 10%. In one embodiment, the reduction is greater than about 20%. In one embodiment, the reduction is greater than about 30%. In one embodiment, the reduction is greater than about 40%. In one embodiment, the reduction is greater than about 50%. In one embodiment, the reduction is greater than about 60%. In one embodiment, the reduction is greater than about 70%. In one embodiment, the reduction is greater than about 80%. In one embodiment, the reduction is greater than about 90%. In one embodiment, the reduction is about 100%. In one embodiment, the reduction is less than about 10%. In one embodiment, the reduction is less than about 20%. In one embodiment, the reduction is less than about 30%. In one embodiment, the reduction is less than about 40%. In one embodiment, the reduction is less than about 50%. In one embodiment, the reduction is less than about 60%. In one embodiment, the reduction is less than about 70%. In one embodiment, the reduction is less than about 80%. In one embodiment, the reduction is less than about 90%. In one embodiment, the reduction is about 100%. In one embodiment, the reduction is about 44.1%. In one embodiment, the reduction is about 43.9%. In one embodiment, the reduction is about 92.9%. In one embodiment, the reduction is about 44.4%. In one embodiment, the reduction is about 49.5%. In one embodiment, the reduction is about 55.3%. In one embodiment, the reduction is about 54%. 
     In one embodiment, the disclosure relates to a method of inhibiting p38α MAPK where inhibiting p38α MAPK regulates leukocyte trafficking. 
     In one embodiment, the disclosure relates to a method of inhibiting p38α MAPK where inhibiting p38α MAPK regulates cytokine expression. 
     Methods of Treatment 
     The compounds and compositions described herein can be used in methods for treating diseases. In some embodiments, the compounds and compositions described herein can be used in methods for treating diseases associated with the up- and/or downregulation of the p38α MAPK protein. 
     In one embodiment, the disclosure relates to a method of treating a disease alleviated by inhibiting the p38α MAPK protein in a patient in need thereof, including administering to the patient a therapeutically effective amount of a p38α MAPK inhibitor, wherein the p38α MAPK inhibitor is a compound capable of binding to a pocket near the ED substrate-docking site of p38α MAPK, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof. In one embodiment, the binding pocket is defined at least by residues R49, H107, L108, and K165 in p38α MAPK. In one embodiment, the binding pocket is defined by residues R49, H107, L108, M109, G110, A157, V158, E163, L164, and K165 in p38α MAPK. In one embodiment, the p38α MAPK inhibitor is a compound of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180, e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044), and formulas 2001-2020, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof. In some embodiments, the p38α MAPK inhibitor is a p38α MAPK selective inhibitor. 
     In one embodiment, the disclosure relates to a method of treating a disease alleviated by inhibiting the p38α MAPK protein in a patient in need thereof, including administering to the patient a therapeutically effective amount of a p38α MAPK inhibitor in a dosage unit form. In one embodiment, the dosage unit comprises a physiologically compatible carrier medium. 
     In one embodiment, the disclosure relates to a method of treating a disease alleviated by inhibiting the p38α MAPK protein in a patient in need thereof, including administering to the patient a therapeutically effective amount of a p38α MAPK inhibitor, wherein the disease is cancer or an inflammatory disease. In some embodiments, the disease is rheumatoid arthritis, a cardiovascular disease, multiple sclerosis, inflammatory bowel disease, chronic obstructive pulmonary disease (COPD), asthma, acute respiratory distress syndrome (ARDS), or acute lung injury (ALI). In one embodiment, the disease is a hyperproliferative diseases. In some embodiments, the hyperproliferative disorder is cancer. In some embodiments, the cancer is pancreatic cancer, breast cancer, prostate cancer, lymphoma, skin cancer, colon cancer, melanoma, malignant melanoma, ovarian cancer, brain cancer, primary brain carcinoma, head-neck cancer, glioma, glioblastoma, liver cancer, bladder cancer, non-small cell lung cancer, head or neck carcinoma, breast carcinoma, ovarian carcinoma, lung carcinoma, small-cell lung carcinoma, Wilms&#39; tumor, cervical carcinoma, testicular carcinoma, bladder carcinoma, pancreatic carcinoma, stomach carcinoma, colon carcinoma, prostatic carcinoma, genitourinary carcinoma, thyroid carcinoma, esophageal carcinoma, myeloma, multiple myeloma, adrenal carcinoma, renal cell carcinoma, endometrial carcinoma, adrenal cortex carcinoma, malignant pancreatic insulinoma, malignant carcinoid carcinoma, choriocarcinoma, mycosis fungoides, malignant hypercalcemia, cervical hyperplasia, leukemia, acute lymphocytic leukemia, chronic lymphocytic leukemia, acute myelogenous leukemia, chronic myelogenous leukemia, chronic granulocytic leukemia, acute granulocytic leukemia, hairy cell leukemia, neuroblastoma, rhabdomyosarcoma, Kaposi&#39;s sarcoma, polycythemia vera, essential thrombocytosis, Hodgkin&#39;s disease, non-Hodgkin&#39;s lymphoma, soft-tissue sarcoma, osteogenic sarcoma, primary macroglobulinemia, or retinoblastoma, and the like. In other embodiments, the cancer is acoustic neuroma, adenocarcinoma, angiosarcoma, astrocytoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, brain cancer, breast cancer, bronchogenic carcinoma, cervical cancer, chordoma, choriocarcinoma, colon cancer, colorectal cancer, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliocarcinoma, ependymoma, epithelial carcinoma, esophageal cancer, Ewing&#39;s tumor, fibrosarcoma, gastric cancer, glioblastoma multiforme, glioma, head and neck cancer, hemangioblastoma, hepatoma, kidney cancer, leiomyosarcoma, liposarcoma, lung cancer, lymphangioendotheliosarcoma, lymphangiosarcoma, medullary carcinoma, medulloblastoma, melanoma, meningioma, mesothelioma, myxosarcoma, nasal cancer, neuroblastoma, oligodendroglioma, oral cancer, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinoma, papillary carcinoma, pinealoma, prostate cancer, rabdomyosarcoma, rectal cancer, renal cell carcinoma, retinoblastoma, sarcoma, sebaceous gland carcinoma, seminoma, skin cancer, squamous cell carcinoma, stomach cancer, sweat gland carcinoma, synovioma, testicular cancer, small cell lung carcinoma, throat cancer, uterine cancer, Wilm&#39;s tumor, blood cancer, acute erythroleukemic leukemia, acute lymphoblastic B-cell leukemia, acute lymphoblastic T-cell leukemia, acute lymphoblastic leukemia, acute megakaryoblastic leukemia, acute monoblastic leukemia, acute myeloblastic leukemia, acute myelomonocytic leukemia, acute nonlymphocytic leukemia, acute promyelocytic leukemia, acute undifferentiated leukemia, chronic lymphocytic leukemia, chronic myelocytic leukemia, hairy cell leukemia, multiple myeloma, heavy chain disease, Hodgkin&#39;s disease, multiple myeloma, non-Hodgkin&#39;s lymphoma, polycythemia vera, or Waldenstrom&#39;s macroglobulinemia. 
     In some embodiments, the hyperproliferative disorder (e.g., cancer) treated by the compounds and compositions described herein includes cells having p38α MAPK protein and/or p38α MAPK related protein expression. 
     In one embodiment, the disclosure relates to a method of treating a disease alleviated by inhibiting the p38α MAPK protein in a patient in need thereof, including administering to the patient a therapeutically effective amount of a p38α MAPK inhibitor, wherein the p38α MAPK inhibitor is a compound capable of binding to a pocket near the ED substrate-docking site of p38α MAPK, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, and one or more additional therapeutic agents, including chemotherapeutic and/or immunotherapeutic agents. 
     Efficacy of the compounds and combinations of compounds described herein in treating the indicated diseases or disorders can be tested using various models known in the art, and described herein, which provide guidance for treatment of human disease. Any and all of the described methods of treatment may include medical follow-up to determine the therapeutic or prophylactic effect brought about in the subject undergoing treatment with the compound(s) and/or composition(s) described herein. 
     Pharmaceutical Compositions 
     In an embodiment, an active pharmaceutical ingredient or combination of active pharmaceutical ingredients, such as any of the p38α MAPK inhibitors of the disclosure, is provided as a pharmaceutically acceptable composition. 
     In one embodiment, the disclosure relates to a pharmaceutical composition including a therapeutically effective amount of a p38α MAPK inhibitor for the treatment of a disease alleviated by inhibiting p38α MAPK activity in a patient in need thereof, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof; and a physiologically compatible carrier medium; wherein the p38α MAPK inhibitor is a compound capable of binding to a pocket near the ED substrate-docking site of p38α MAPK. In one embodiment, the binding pocket is defined at least by residues R49, H107, L108, and K165 in p38α MAPK. In one embodiment, the binding pocket is defined by residues R49, H107, L108, M109, G110, A157, V158, E163, L164, and K165 in p38α MAPK. In one embodiment, the p38α MAPK inhibitor is a compound of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof. In some embodiments, the p38α MAPK inhibitor is a p38α MAPK selective inhibitor. 
     In one embodiment, the disclosure relates to a pharmaceutical composition including a therapeutically effective amount of a p38α MAPK inhibitor for the treatment of a disease alleviated by inhibiting p38α MAPK activity in a patient in need thereof, or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof; and a physiologically compatible carrier medium, wherein the disease is cancer or an inflammatory disease. In one embodiment, the disease is rheumatoid arthritis, a cardiovascular disease, multiple sclerosis, inflammatory bowel disease, chronic obstructive pulmonary disease (COPD), asthma, acute respiratory distress syndrome (ARDS), or acute lung injury (ALI). In one embodiment, the diseases is a cancer such as acoustic neuroma, adenocarcinoma, angiosarcoma, astrocytoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, brain cancer, breast cancer, bronchogenic carcinoma, cervical cancer, chordoma, choriocarcinoma, colon cancer, colorectal cancer, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliocarcinoma, ependymoma, epithelial carcinoma, esophageal cancer, Ewing&#39;s tumor, fibrosarcoma, gastric cancer, glioblastoma multiforme, glioma, head and neck cancer, hemangioblastoma, hepatoma, kidney cancer, leiomyosarcoma, liposarcoma, lung cancer, lymphangioendotheliosarcoma, lymphangiosarcoma, medullary carcinoma, medulloblastoma, melanoma, meningioma, mesothelioma, myxosarcoma, nasal cancer, neuroblastoma, oligodendroglioma, oral cancer, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinoma, papillary carcinoma, pinealoma, prostate cancer, rabdomyosarcoma, rectal cancer, renal cell carcinoma, retinoblastoma, sarcoma, sebaceous gland carcinoma, seminoma, skin cancer, squamous cell carcinoma, stomach cancer, sweat gland carcinoma, synovioma, testicular cancer, small cell lung carcinoma, throat cancer, uterine cancer, Wilm&#39;s tumor, blood cancer, acute erythroleukemic leukemia, acute lymphoblastic B-cell leukemia, acute lymphoblastic T-cell leukemia, acute lymphoblastic leukemia, acute megakaryoblastic leukemia, acute monoblastic leukemia, acute myeloblastic leukemia, acute myelomonocytic leukemia, acute nonlymphocytic leukemia, acute promyelocytic leukemia, acute undifferentiated leukemia, chronic lymphocytic leukemia, chronic myelocytic leukemia, hairy cell leukemia, multiple myeloma, heavy chain disease, Hodgkin&#39;s disease, multiple myeloma, non-Hodgkin&#39;s lymphoma, polycythemia vera, or Waldenstrom&#39;s macroglobulinemia. 
     In some embodiments, the concentration of each of the active pharmaceutical ingredients provided in the pharmaceutical compositions of the disclosure, such as any of the p38α MAPK inhibitors of the disclosure, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, is less than, for example, 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 19%, 18%, 17%, 16%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, 0.4%, 0.3%, 0.2%, 0.1%, 0.09%, 0.08%, 0.07%, 0.06%, 0.05%, 0.04%, 0.03%, 0.02%, 0.01%, 0.009%, 0.008%, 0.007%, 0.006%, 0.005%, 0.004%, 0.003%, 0.002%, 0.001%, 0.0009%, 0.0008%, 0.0007%, 0.0006%, 0.0005%, 0.0004%, 0.0003%, 0.0002% or 0.0001% w/w, w/v, or v/v of the pharmaceutical composition. 
     In some embodiments, the concentration of each of the active pharmaceutical ingredients provided in the pharmaceutical compositions of the disclosure, such as any of the p38α MAPK inhibitors of the disclosure, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, is greater than 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 19.75%, 19.50%, 19.25% 19%, 18.75%, 18.50%, 18.25% 18%, 17.75%, 17.50%, 17.25% 17%, 16.75%, 16.50%, 16.25% 16%, 15.75%, 15.50%, 15.25% 15%, 14.75%, 14.50%, 14.25% 14%, 13.75%, 13.50%, 13.25% 13%, 12.75%, 12.50%, 12.25% 12%, 11.75%, 11.50%, 11.25% 11%, 10.75%, 10.50%, 10.25% 10%, 9.75%, 9.50%, 9.25% 9%, 8.75%, 8.50%, 8.25% 8%, 7.75%, 7.50%, 7.25% 7%, 6.75%, 6.50%, 6.25% 6%, 5.75%, 5.50%, 5.25% 5%, 4.75%, 4.50%, 4.25%, 4%, 3.75%, 3.50%, 3.25%, 3%, 2.75%, 2.50%, 2.25%, 2%, 1.75%, 1.50%, 125%, 1%, 0.5%, 0.4%, 0.3%, 0.2%, 0.1%, 0.09%, 0.08%, 0.07%, 0.06%, 0.05%, 0.04%, 0.03%, 0.02%, 0.01%, 0.009%, 0.008%, 0.007%, 0.006%, 0.005%, 0.004%, 0.003%, 0.002%, 0.001%, 0.0009%, 0.0008%, 0.0007%, 0.0006%, 0.0005%, 0.0004%, 0.0003%, 0.0002% or 0.0001% w/w, w/v, or v/v of the pharmaceutical composition. 
     In some embodiments, the concentration of each of the active pharmaceutical ingredients provided in the pharmaceutical compositions of the disclosure, such as any of the p38α MAPK inhibitors of the disclosure, for example any of the compounds of any of Formulas A, I, II, 1001-1180, in particular of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, is in the range from about 0.0001% to about 50%, about 0.001% to about 40%, about 0.01% to about 30%, about 0.02% to about 29%, about 0.03% to about 28%, about 0.04% to about 27%, about 0.05% to about 26%, about 0.06% to about 25%, about 0.07% to about 24%, about 0.08% to about 23%, about 0.09% to about 22%, about 0.1% to about 21%, about 0.2% to about 20%, about 0.3% to about 19%, about 0.4% to about 18%, about 0.5% to about 17%, about 0.6% to about 16%, about 0.7% to about 15%, about 0.8% to about 14%, about 0.9% to about 12% or about 1% to about 10% w/w, w/v, or v/v of the pharmaceutical composition. 
     In some embodiments, the concentration of each of the active pharmaceutical ingredients provided in the pharmaceutical compositions of the disclosure, such as any of the p38α MAPK inhibitors of the disclosure, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, is in the range from about 0.001% to about 10%, about 0.01% to about 5%, about 0.02% to about 4.5%, about 0.03% to about 4%, about 0.04% to about 3.5%, about 0.05% to about 3%, about 0.06% to about 2.5%, about 0.07% to about 2%, about 0.08% to about 1.5%, about 0.09% to about 1%, about 0.1% to about 0.9% w/w, w/v, or v/v of the pharmaceutical composition. 
     In some embodiments, the amount of each of the active pharmaceutical ingredients provided in the pharmaceutical compositions of the disclosure, such as any of the foregoing p38α MAPK inhibitors of the disclosure, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, is equal to or less than 10 g, 9.5 g, 9.0 g, 8.5 g, 8.0 g, 7.5 g, 7.0 g, 6.5 g, 6.0 g, 5.5 g, 5.0 g, 4.5 g, 4.0 g, 3.5 g, 3.0 g, 2.5 g, 2.0 g, 1.5 g, 1.0 g, 0.95 g, 0.9 g, 0.85 g, 0.8 g, 0.75 g, 0.7 g, 0.65 g, 0.6 g, 0.55 g, 0.5 g, 0.45 g, 0.4 g, 0.35 g, 0.3 g, 0.25 g, 0.2 g, 0.15 g, 0.1 g, 0.09 g, 0.08 g, 0.07 g, 0.06 g, 0.05 g, 0.04 g, 0.03 g, 0.02 g, 0.01 g, 0.009 g, 0.008 g, 0.007 g, 0.006 g, 0.005 g, 0.004 g, 0.003 g, 0.002 g, 0.001 g, 0.0009 g, 0.0008 g, 0.0007 g, 0.0006 g, 0.0005 g, 0.0004 g, 0.0003 g, 0.0002 g, or 0.0001 g. 
     In some embodiments, the amount of each of the active pharmaceutical ingredients provided in the pharmaceutical compositions of the disclosure, such as any of the p38α MAPK inhibitors of the disclosure, for example any of the compounds of any of Formulas A, I, II, 1001-1180, in particular of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, is more than 0.0001 g, 0.0002 g, 0.0003 g, 0.0004 g, 0.0005 g, 0.0006 g, 0.0007 g, 0.0008 g, 0.0009 g, 0.001 g, 0.0015 g, 0.002 g, 0.0025 g, 0.003 g, 0.0035 g, 0.004 g, 0.0045 g, 0.005 g, 0.0055 g, 0.006 g, 0.0065 g, 0.007 g, 0.0075 g, 0.008 g, 0.0085 g, 0.009 g, 0.0095 g, 0.01 g, 0.015 g, 0.02 g, 0.025 g, 0.03 g, 0.035 g, 0.04 g, 0.045 g, 0.05 g, 0.055 g, 0.06 g, 0.065 g, 0.07 g, 0.075 g, 0.08 g, 0.085 g, 0.09 g, 0.095 g, 0.1 g, 0.15 g, 0.2 g, 0.25 g, 0.3 g, 0.35 g, 0.4 g, 0.45 g, 0.5 g, 0.55 g, 0.6 g, 0.65 g, 0.7 g, 0.75 g, 0.8 g, 0.85 g, 0.9 g, 0.95 g, 1 g, 1.5 g, 2 g, 2.5, 3 g, 3.5, 4 g, 4.5 g, 5 g, 5.5 g, 6 g, 6.5 g, 7 g, 7.5 g, 8 g, 8.5 g, 9 g, 9.5 g, or 10 g. 
     Each of the active pharmaceutical ingredients according to the disclosure is effective over a wide dosage range. For example, in the treatment of adult humans, dosages independently range from 0.01 to 1000 mg, from 0.5 to 100 mg, from 1 to 50 mg per day, and from 5 to 40 mg per day are examples of dosages that may be used. The exact dosage will depend upon the route of administration, the form in which the compound is administered, the gender and age of the subject to be treated, the body weight of the subject to be treated, and the preference and experience of the attending physician. The clinically-established dosages of the p38α MAPK inhibitors of the disclosure may also be used if appropriate. 
     In an embodiment, the molar ratio of two active pharmaceutical ingredients in the pharmaceutical compositions is in the range from 10:1 to 1:10, preferably from 2.5:1 to 1:2.5, and more preferably about 1:1. In an embodiment, the weight ratio of the molar ratio of two active pharmaceutical ingredients in the pharmaceutical compositions is selected from the group consisting of 20:1, 19:1, 18:1, 17:1, 16:1, 15:1, 14:1, 13:1, 12:1, 11:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, and 1:20. In an embodiment, the weight ratio of the molar ratio of two active pharmaceutical ingredients in the pharmaceutical compositions is selected from the group consisting of 20:1, 19:1, 18:1, 17:1, 16:1, 15:1, 14:1, 13:1, 12:1, 11:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, and 1:20. 
     In an embodiment, the pharmaceutical compositions described herein, such as any of the p38α MAPK inhibitors of the disclosure, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, are for use in the treatment of an inflammatory disease. In an embodiment, the pharmaceutical compositions described herein, such as any of the p38α MAPK inhibitors of the disclosure, are for use in the treatment of rheumatoid arthritis, a cardiovascular disease, multiple sclerosis, inflammatory bowel disease, chronic obstructive pulmonary disease (COPD), asthma, acute respiratory distress syndrome (ARDS), or acute lung injury (ALI). 
     In an embodiment, the pharmaceutical compositions described herein, such as any of the p38α MAPK inhibitors of the disclosure, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, are for use in the treatment of hyperproliferative disorders associated with the overexpression or up- and/or downregulation p38α MAPK protein. In a some embodiments, the pharmaceutical compositions described herein are for use in the treatment of a cancer associated with overexpression or up- and/or downregulation of p38α MAPK protein, such as pancreatic cancer, breast cancer, prostate cancer, lymphoma, skin cancer, colon cancer, melanoma, malignant melanoma, ovarian cancer, brain cancer, primary brain carcinoma, head-neck cancer, glioma, glioblastoma, liver cancer, bladder cancer, non-small cell lung cancer, head or neck carcinoma, breast carcinoma, ovarian carcinoma, lung carcinoma, small-cell lung carcinoma, Wilms&#39; tumor, cervical carcinoma, testicular carcinoma, bladder carcinoma, pancreatic carcinoma, stomach carcinoma, colon carcinoma, prostatic carcinoma, genitourinary carcinoma, thyroid carcinoma, esophageal carcinoma, myeloma, multiple myeloma, adrenal carcinoma, renal cell carcinoma, endometrial carcinoma, adrenal cortex carcinoma, malignant pancreatic insulinoma, malignant carcinoid carcinoma, choriocarcinoma, mycosis fungoides, malignant hypercalcemia, cervical hyperplasia, leukemia, acute lymphocytic leukemia, chronic lymphocytic leukemia, acute myelogenous leukemia, chronic myelogenous leukemia, chronic granulocytic leukemia, acute granulocytic leukemia, hairy cell leukemia, neuroblastoma, rhabdomyosarcoma, Kaposi&#39;s sarcoma, polycythemia vera, essential thrombocytosis, Hodgkin&#39;s disease, non-Hodgkin&#39;s lymphoma, soft-tissue sarcoma, osteogenic sarcoma, primary macroglobulinemia, or retinoblastoma. 
     Described below are non-limiting pharmaceutical compositions and methods for preparing the same. 
     Pharmaceutical Compositions for Oral Administration 
     In an embodiment, the disclosure provides a pharmaceutical composition for oral administration containing the active pharmaceutical ingredient or combination of active pharmaceutical ingredients, such as the p38α MAPK inhibitors described herein, and a pharmaceutical excipient suitable for oral administration. 
     In some embodiments, the disclosure provides a solid pharmaceutical composition for oral administration containing: (i) an effective amount of an active pharmaceutical ingredient or combination of active pharmaceutical ingredients, and (ii) a pharmaceutical excipient suitable for oral administration. In selected embodiments, the composition further contains (iii) an effective amount of a third active pharmaceutical ingredient, and optionally (iv) an effective amount of a fourth active pharmaceutical ingredient. 
     In some embodiments, the pharmaceutical composition may be a liquid pharmaceutical composition suitable for oral consumption. Pharmaceutical compositions of the disclosure suitable for oral administration can be presented as discrete dosage forms, such as capsules, sachets, or tablets, or liquids or aerosol sprays each containing a predetermined amount of an active ingredient as a powder or in granules, a solution, or a suspension in an aqueous or non-aqueous liquid, an oil-in-water emulsion, a water-in-oil liquid emulsion, powders for reconstitution, powders for oral consumptions, bottles (including powders or liquids in a bottle), orally dissolving films, lozenges, pastes, tubes, gums, and packs. Such dosage forms can be prepared by any of the methods of pharmacy, but all methods include the step of bringing the active ingredient(s) into association with the carrier, which constitutes one or more necessary ingredients. In general, the compositions are prepared by uniformly and intimately admixing the active ingredient(s) with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product into the desired presentation. For example, a tablet can be prepared by compression or molding, optionally with one or more accessory ingredients. Compressed tablets can be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as powder or granules, optionally mixed with an excipient such as, but not limited to, a binder, a lubricant, an inert diluent, and/or a surface active or dispersing agent. Molded tablets can be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. 
     The disclosure further encompasses anhydrous pharmaceutical compositions and dosage forms since water can facilitate the degradation of some compounds. For example, water may be added (e.g., 5%) in the pharmaceutical arts as a means of simulating long-term storage in order to determine characteristics such as shelf-life or the stability of formulations over time. Anhydrous pharmaceutical compositions and dosage forms of the disclosure can be prepared using anhydrous or low moisture containing ingredients and low moisture or low humidity conditions. Pharmaceutical compositions and dosage forms of the disclosure which contain lactose can be made anhydrous if substantial contact with moisture and/or humidity during manufacturing, packaging, and/or storage is expected. An anhydrous pharmaceutical composition may be prepared and stored such that its anhydrous nature is maintained. Accordingly, anhydrous compositions may be packaged using materials known to prevent exposure to water such that they can be included in suitable formulary kits. Examples of suitable packaging include, but are not limited to, hermetically sealed foils, plastic or the like, unit dose containers, blister packs, and strip packs. 
     Each of the active pharmaceutical ingredients can be combined in an intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques. The carrier can take a wide variety of forms depending on the form of preparation desired for administration. In preparing the compositions for an oral dosage form, any of the usual pharmaceutical media can be employed as carriers, such as, for example, water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents, and the like in the case of oral liquid preparations (such as suspensions, solutions, and elixirs) or aerosols; or carriers such as starches, sugars, micro-crystalline cellulose, diluents, granulating agents, lubricants, binders, and disintegrating agents can be used in the case of oral solid preparations, in some embodiments without employing the use of lactose. For example, suitable carriers include powders, capsules, and tablets, with the solid oral preparations. If desired, tablets can be coated by standard aqueous or nonaqueous techniques. 
     Binders suitable for use in pharmaceutical compositions and dosage forms include, but are not limited to, corn starch, potato starch, or other starches, gelatin, natural and synthetic gums such as acacia, sodium alginate, alginic acid, other alginates, powdered tragacanth, guar gum, cellulose and its derivatives (e.g., ethyl cellulose, cellulose acetate, carboxymethyl cellulose calcium, sodium carboxymethyl cellulose), polyvinyl pyrrolidone, methyl cellulose, pre-gelatinized starch, hydroxypropyl methyl cellulose, microcrystalline cellulose, and mixtures thereof. 
     Examples of suitable fillers for use in the pharmaceutical compositions and dosage forms disclosed herein include, but are not limited to, talc, calcium carbonate (e.g., granules or powder), microcrystalline cellulose, powdered cellulose, dextrates, kaolin, mannitol, silicic acid, sorbitol, starch, pre-gelatinized starch, and mixtures thereof. 
     Disintegrants may be used in the compositions of the disclosure to provide tablets that disintegrate when exposed to an aqueous environment. Too much of a disintegrant may produce tablets which disintegrate in the bottle. Too little may be insufficient for disintegration to occur, thus altering the rate and extent of release of the active ingredients from the dosage form. Thus, a sufficient amount of disintegrant that is neither too little nor too much to detrimentally alter the release of the active ingredient(s) may be used to form the dosage forms of the compounds disclosed herein. The amount of disintegrant used may vary based upon the type of formulation and mode of administration, and may be readily discernible to those of ordinary skill in the art. About 0.5 to about 15 weight percent of disintegrant, or about 1 to about 5 weight percent of disintegrant, may be used in the pharmaceutical composition. Disintegrants that can be used to form pharmaceutical compositions and dosage forms of the disclosure include, but are not limited to, agar-agar, alginic acid, calcium carbonate, microcrystalline cellulose, croscarmellose sodium, crospovidone, polacrilin potassium, sodium starch glycolate, potato or tapioca starch, other starches, pre-gelatinized starch, other starches, clays, other algins, other celluloses, gums or mixtures thereof. 
     Lubricants which can be used to form pharmaceutical compositions and dosage forms of the disclosure include, but are not limited to, calcium stearate, magnesium stearate, sodium stearyl fumarate, mineral oil, light mineral oil, glycerin, sorbitol, mannitol, polyethylene glycol, other glycols, stearic acid, sodium lauryl sulfate, talc, hydrogenated vegetable oil (e.g., peanut oil, cottonseed oil, sunflower oil, sesame oil, olive oil, corn oil, and soybean oil), zinc stearate, ethyl oleate, ethylaureate, agar, or mixtures thereof. Additional lubricants include, for example, a syloid silica gel, a coagulated aerosol of synthetic silica, silicified microcrystalline cellulose, or mixtures thereof. A lubricant can optionally be added in an amount of less than about 0.5% or less than about 1% (by weight) of the pharmaceutical composition. 
     When aqueous suspensions and/or elixirs are desired for oral administration, the active pharmaceutical ingredient(s) may be combined with various sweetening or flavoring agents, coloring matter or dyes and, if so desired, emulsifying and/or suspending agents, together with such diluents as water, ethanol, propylene glycol, glycerin and various combinations thereof. 
     The tablets can be uncoated or coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate can be employed. Formulations for oral use can also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example, peanut oil, liquid paraffin or olive oil. 
     Surfactants which can be used to form pharmaceutical compositions and dosage forms of the disclosure include, but are not limited to, hydrophilic surfactants, lipophilic surfactants, and mixtures thereof. That is, a mixture of hydrophilic surfactants may be employed, a mixture of lipophilic surfactants may be employed, or a mixture of at least one hydrophilic surfactant and at least one lipophilic surfactant may be employed. 
     A suitable hydrophilic surfactant may generally have an HLB value of at least 10, while suitable lipophilic surfactants may generally have an HLB value of or less than about 10. An empirical parameter used to characterize the relative hydrophilicity and hydrophobicity of non-ionic amphiphilic compounds is the hydrophilic-lipophilic balance (“HLB” value). Surfactants with lower HLB values are more lipophilic or hydrophobic, and have greater solubility in oils, while surfactants with higher HLB values are more hydrophilic, and have greater solubility in aqueous solutions. Hydrophilic surfactants are generally considered to be those compounds having an HLB value greater than about 10, as well as anionic, cationic, or zwitterionic compounds for which the HLB scale is not generally applicable. Similarly, lipophilic (i.e., hydrophobic) surfactants are compounds having an HLB value equal to or less than about 10. However, HLB value of a surfactant is merely a rough guide generally used to enable formulation of industrial, pharmaceutical and cosmetic emulsions. 
     Hydrophilic surfactants may be either ionic or non-ionic. Suitable ionic surfactants include, but are not limited to, alkylammonium salts; fusidic acid salts; fatty acid derivatives of amino acids, oligopeptides, and polypeptides; glyceride derivatives of amino acids, oligopeptides, and polypeptides; lecithins and hydrogenated lecithins; lysolecithins and hydrogenated lysolecithins; phospholipids and derivatives thereof; lysophospholipids and derivatives thereof; camitine fatty acid ester salts; salts of alkylsulfates; fatty acid salts; sodium docusate; acyl-lactylates; mono- and di-acetylated tartaric acid esters of mono- and di-glycerides; succinylated mono- and di-glycerides; citric acid esters of mono- and di-glycerides; and mixtures thereof. 
     Within the aforementioned group, ionic surfactants include, by way of example: lecithins, lysolecithin, phospholipids, lysophospholipids and derivatives thereof; camitine fatty acid ester salts; salts of alkylsulfates; fatty acid salts; sodium docusate; acylactylates; mono- and di-acetylated tartaric acid esters of mono- and di-glycerides; succinylated mono- and di-glycerides; citric acid esters of mono- and di-glycerides; and mixtures thereof. 
     Ionic surfactants may be the ionized forms of lecithin, lysolecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidic acid, phosphatidylserine, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidic acid, lysophosphatidylserine, PEG-phosphatidylethanolamine, PVP-phosphatidylethanolamine, lactylic esters of fatty acids, stearoyl-2-lactylate, stearoyl lactylate, succinylated monoglycerides, mono/diacetylated tartaric acid esters of mono/diglycerides, citric acid esters of mono/diglycerides, cholylsarcosine, caproate, caprylate, caprate, laurate, myristate, palmitate, oleate, ricinoleate, linoleate, linolenate, stearate, lauryl sulfate, teracecyl sulfate, docusate, lauroyl camitines, palmitoyl camitines, myristoyl camitines, and salts and mixtures thereof. 
     Hydrophilic non-ionic surfactants may include, but not limited to, alkylglucosides; alkylmaltosides; alkylthioglucosides; lauryl macrogolglycerides; polyoxyalkylene alkyl ethers such as polyethylene glycol alkyl ethers; polyoxyalkylene alkylphenols such as polyethylene glycol alkyl phenols; polyoxyalkylene alkyl phenol fatty acid esters such as polyethylene glycol fatty acids monoesters and polyethylene glycol fatty acids diesters; polyethylene glycol glycerol fatty acid esters; polyglycerol fatty acid esters; polyoxyalkylene sorbitan fatty acid esters such as polyethylene glycol sorbitan fatty acid esters; hydrophilic transesterification products of a polyol with at least one member of the group consisting of glycerides, vegetable oils, hydrogenated vegetable oils, fatty acids, and sterols; polyoxyethylene sterols, derivatives, and analogs thereof; polyoxyethylated vitamins and derivatives thereof; polyoxyethylene-polyoxypropylene block copolymers; and mixtures thereof; polyethylene glycol sorbitan fatty acid esters and hydrophilic transesterification products of a polyol with at least one member of the group consisting of triglycerides, vegetable oils, and hydrogenated vegetable oils. The polyol may be glycerol, ethylene glycol, polyethylene glycol, sorbitol, propylene glycol, pentaerythritol, or a saccharide. 
     Other hydrophilic-non-ionic surfactants include, without limitation, PEG-10 laurate, PEG-12 laurate, PEG-20 laurate, PEG-32 laurate, PEG-32 dilaurate, PEG-12 oleate, PEG-15 oleate, PEG-20 oleate, PEG-20 dioleate, PEG-32 oleate, PEG-200 oleate, PEG-400 oleate, PEG-15 stearate, PEG-32 distearate, PEG-40 stearate, PEG-100 stearate, PEG-20 dilaurate, PEG-25 glyceryl trioleate, PEG-32 dioleate, PEG-20 glyceryl laurate, PEG-30 glyceryl laurate, PEG-20 glyceryl stearate, PEG-20 glyceryl oleate, PEG-30 glyceryl oleate, PEG-30 glyceryl laurate, PEG-40 glyceryl laurate, PEG-40 palm kernel oil, PEG-50 hydrogenated castor oil, PEG-40 castor oil, PEG-35 castor oil, PEG-60 castor oil, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PEG-60 corn oil, PEG-6 caprate/caprylate glycerides, PEG-8 caprate/caprylate glycerides, polyglyceryl-10 laurate, PEG-30 cholesterol, PEG-25 phyto sterol, PEG-30 soya sterol, PEG-20 trioleate, PEG-40 sorbitan oleate, PEG-80 sorbitan laurate, polysorbate 20, polysorbate 80, POE-9 lauryl ether, POE-23 lauryl ether, POE-10 oleyl ether, POE-20 oleyl ether, POE-20 stearyl ether, tocopheryl PEG-100 succinate, PEG-24 cholesterol, polyglyceryl-10 oleate, Tween 40, Tween 60, sucrose monostearate, sucrose monolaurate, sucrose monopalmitate, PEG 10-100 nonyl phenol series, PEG 15-100 octyl phenol series, and poloxamers. 
     Suitable lipophilic surfactants include, by way of example only: fatty alcohols; glycerol fatty acid esters; acetylated glycerol fatty acid esters; lower alcohol fatty acids esters; propylene glycol fatty acid esters; sorbitan fatty acid esters; polyethylene glycol sorbitan fatty acid esters; sterols and sterol derivatives; polyoxyethylated sterols and sterol derivatives; polyethylene glycol alkyl ethers; sugar esters; sugar ethers; lactic acid derivatives of mono- and di-glycerides; hydrophobic transesterification products of a polyol with at least one member of the group consisting of glycerides, vegetable oils, hydrogenated vegetable oils, fatty acids and sterols; oil-soluble vitamins/vitamin derivatives; and mixtures thereof. Within this group, preferred lipophilic surfactants include glycerol fatty acid esters, propylene glycol fatty acid esters, and mixtures thereof, or are hydrophobic transesterification products of a polyol with at least one member of the group consisting of vegetable oils, hydrogenated vegetable oils, and triglycerides. 
     In an embodiment, the composition may include a solubilizer to ensure good solubilization and/or dissolution of the compound of the present disclosure and to minimize precipitation of the compound of the present disclosure. This can be especially important for compositions for non-oral use—e.g., compositions for injection. A solubilizer may also be added to increase the solubility of the hydrophilic drug and/or other components, such as surfactants, or to maintain the composition as a stable or homogeneous solution or dispersion. 
     Examples of suitable solubilizers include, but are not limited to, the following: alcohols and polyols, such as ethanol, isopropanol, butanol, benzyl alcohol, ethylene glycol, propylene glycol, butanediols and isomers thereof, glycerol, pentaerythritol, sorbitol, mannitol, transcutol, dimethyl isosorbide, polyethylene glycol, polypropylene glycol, polyvinylalcohol, hydroxypropyl methylcellulose and other cellulose derivatives, cyclodextrins and cyclodextrin derivatives; ethers of polyethylene glycols having an average molecular weight of about 200 to about 6000, such as tetrahydrofurfuryl alcohol PEG ether (glycofurol) or methoxy PEG; amides and other nitrogen-containing compounds such as 2-pyrrolidone, 2-piperidone, ε-caprolactam, N-alkylpyrrolidone, N-hydroxyalkylpyrrolidone, N-alkylpiperidone, N-alkylcaprolactam, dimethylacetamide and polyvinylpyrrolidone; esters such as ethyl propionate, tributylcitrate, acetyl triethylcitrate, acetyl tributyl citrate, triethylcitrate, ethyl oleate, ethyl caprylate, ethyl butyrate, triacetin, propylene glycol monoacetate, propylene glycol diacetate, ε-caprolactone and isomers thereof, -valerolactone and isomers thereof, β-butyrolactone and isomers thereof; and other solubilizers known in the art, such as dimethyl acetamide, dimethyl isosorbide, N-methyl pyrrolidones, monooctanoin, diethylene glycol monoethyl ether, and water. 
     Mixtures of solubilizers may also be used. Examples include, but not limited to, triacetin, triethylcitrate, ethyl oleate, ethyl caprylate, dimethylacetamide, N-methylpyrrolidone, N-hydroxyethylpyrrolidone, polyvinylpyrrolidone, hydroxypropyl methylcellulose, hydroxypropyl cyclodextrins, ethanol, polyethylene glycol 200-100, glycofurol, transcutol, propylene glycol, and dimethyl isosorbide. Particularly preferred solubilizers include sorbitol, glycerol, triacetin, ethyl alcohol, PEG-400, glycofurol and propylene glycol. 
     The amount of solubilizer that can be included is not particularly limited. The amount of a given solubilizer may be limited to a bioacceptable amount, which may be readily determined by one of skill in the art. In some circumstances, it may be advantageous to include amounts of solubilizers far in excess of bioacceptable amounts, for example to maximize the concentration of the drug, with excess solubilizer removed prior to providing the composition to a patient using conventional techniques, such as distillation or evaporation. Thus, if present, the solubilizer can be in a weight ratio of 10%, 25%, 50%, 100%, or up to about 200% by weight, based on the combined weight of the drug, and other excipients. If desired, very small amounts of solubilizer may also be used, such as 5%, 2%, 1% or even less. Typically, the solubilizer may be present in an amount of about 1% to about 100%, more typically about 5% to about 25% by weight. 
     The composition can further include one or more pharmaceutically acceptable additives and excipients. Such additives and excipients include, without limitation, detackifiers, anti-foaming agents, buffering agents, polymers, antioxidants, preservatives, chelating agents, viscomodulators, tonicifiers, flavorants, colorants, odorants, opacifiers, suspending agents, binders, fillers, plasticizers, lubricants, and mixtures thereof. 
     In addition, an acid or a base may be incorporated into the composition to facilitate processing, to enhance stability, or for other reasons. Examples of pharmaceutically acceptable bases include amino acids, amino acid esters, ammonium hydroxide, potassium hydroxide, sodium hydroxide, sodium hydrogen carbonate, aluminum hydroxide, calcium carbonate, magnesium hydroxide, magnesium aluminum silicate, synthetic aluminum silicate, synthetic hydrocalcite, magnesium aluminum hydroxide, diisopropylethylamine, ethanolamine, ethylenediamine, triethanolamine, triethylamine, triisopropanolamine, trimethylamine, tris(hydroxymethyl)aminomethane (TRIS) and the like. Also suitable are bases that are salts of a pharmaceutically acceptable acid, such as acetic acid, acrylic acid, adipic acid, alginic acid, alkanesulfonic acid, amino acids, ascorbic acid, benzoic acid, boric acid, butyric acid, carbonic acid, citric acid, fatty acids, formic acid, fumaric acid, gluconic acid, hydroquinosulfonic acid, isoascorbic acid, lactic acid, maleic acid, oxalic acid, para-bromophenylsulfonic acid, propionic acid, p-toluenesulfonic acid, salicylic acid, stearic acid, succinic acid, tannic acid, tartaric acid, thioglycolic acid, toluenesulfonic acid, uric acid, and the like. Salts of polyprotic acids, such as sodium phosphate, disodium hydrogen phosphate, and sodium dihydrogen phosphate can also be used. When the base is a salt, the cation can be any convenient and pharmaceutically acceptable cation, such as ammonium, alkali metals and alkaline earth metals. Example may include, but not limited to, sodium, potassium, lithium, magnesium, calcium and ammonium. 
     Suitable acids are pharmaceutically acceptable organic or inorganic acids. Examples of suitable inorganic acids include hydrochloric acid, hydrobromic acid, hydriodic acid, sulfuric acid, nitric acid, boric acid, phosphoric acid, and the like. Examples of suitable organic acids include acetic acid, acrylic acid, adipic acid, alginic acid, alkanesulfonic acids, amino acids, ascorbic acid, benzoic acid, boric acid, butyric acid, carbonic acid, citric acid, fatty acids, formic acid, fumaric acid, gluconic acid, hydroquinosulfonic acid, isoascorbic acid, lactic acid, maleic acid, methanesulfonic acid, oxalic acid, para-bromophenylsulfonic acid, propionic acid, p-toluenesulfonic acid, salicylic acid, stearic acid, succinic acid, tannic acid, tartaric acid, thioglycolic acid, toluenesulfonic acid and uric acid. 
     Pharmaceutical Compositions for Injection 
     In some embodiments, a pharmaceutical composition is provided for injection containing an active pharmaceutical ingredient or combination of active pharmaceutical ingredients, such as a p38α MAPK inhibitor, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, and a pharmaceutical excipient suitable for injection. 
     The forms in which the compositions of the present disclosure may be incorporated for administration by injection include aqueous or oil suspensions, or emulsions, with sesame oil, corn oil, cottonseed oil, or peanut oil, as well as elixirs, mannitol, dextrose, or a sterile aqueous solution, and similar pharmaceutical vehicles. 
     Aqueous solutions in saline are also conventionally used for injection. Ethanol, glycerol, propylene glycol and liquid polyethylene glycol (and suitable mixtures thereof), cyclodextrin derivatives, and vegetable oils may also be employed. The proper fluidity can be maintained, for example, by the use of a coating, such as lecithin, for the maintenance of the required particle size in the case of dispersion and by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, and thimerosal. 
     Sterile injectable solutions are prepared by incorporating an active pharmaceutical ingredient or combination of active pharmaceutical ingredients in the required amounts in the appropriate solvent with various other ingredients as enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, certain desirable methods of preparation are vacuum-drying and freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. 
     Pharmaceutical Compositions for Topical Delivery 
     In some embodiments, a pharmaceutical composition is provided for transdermal delivery containing an active pharmaceutical ingredient or combination of active pharmaceutical ingredients, such as p38α MAPK inhibitors described herein, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, and a pharmaceutical excipient suitable for transdermal delivery. 
     Compositions of the present disclosure can be formulated into preparations in solid, semi-solid, or liquid forms suitable for local or topical administration, such as gels, water soluble jellies, creams, lotions, suspensions, foams, powders, slurries, ointments, solutions, oils, pastes, suppositories, sprays, emulsions, saline solutions, dimethylsulfoxide (DMSO)-based solutions. In general, carriers with higher densities are capable of providing an area with a prolonged exposure to the active ingredients. In contrast, a solution formulation may provide more immediate exposure of the active ingredient to the chosen area. 
     The pharmaceutical compositions also may comprise suitable solid or gel phase carriers or excipients, which are compounds that allow increased penetration of, or assist in the delivery of, therapeutic molecules across the stratum comeum permeability barrier of the skin. There are many of these penetration-enhancing molecules known to those trained in the art of topical formulation. Examples of such carriers and excipients include, but are not limited to, humectants (e.g., urea), glycols (e.g., propylene glycol), alcohols (e.g., ethanol), fatty acids (e.g., oleic acid), surfactants (e.g., isopropyl myristate and sodium lauryl sulfate), pyrrolidones, glycerol monolaurate, sulfoxides, terpenes (e.g., menthol), amines, amides, alkanes, alkanols, water, calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols. 
     Another exemplary formulation for use in the methods of the present disclosure employs transdermal delivery devices (“patches”). Such transdermal patches may be used to provide continuous or discontinuous infusion of an active pharmaceutical ingredient or combination of active pharmaceutical ingredients in controlled amounts, either with or without another active pharmaceutical ingredient. 
     The construction and use of transdermal patches for the delivery of pharmaceutical agents is well known in the art. See, e.g., U.S. Pat. Nos. 5,023,252; 4,992,445; and 5,001,139, the entirety of which are incorporated herein by reference. Such patches may be constructed for continuous, pulsatile, or on demand delivery of pharmaceutical agents. 
     Pharmaceutical Compositions for Inhalation 
     Compositions for inhalation or insufflation include solutions and suspensions in pharmaceutically acceptable, aqueous or organic solvents, or mixtures thereof, and powders. The liquid or solid compositions may contain suitable pharmaceutically acceptable excipients as described supra and the p38α MAPK inhibitors described herein, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020. Preferably the compositions are administered by the oral or nasal respiratory route for local or systemic effect. Compositions in preferably pharmaceutically acceptable solvents may be nebulized by use of inert gases. Nebulized solutions may be inhaled directly from the nebulizing device or the nebulizing device may be attached to a face mask tent, or intermittent positive pressure breathing machine. Solution, suspension, or powder compositions may be administered, preferably orally or nasally, from devices that deliver the formulation in an appropriate manner. Dry powder inhalers may also be used to provide inhaled delivery of the compositions. 
     Other Pharmaceutical Compositions 
     Pharmaceutical compositions of the p38α, MAPK inhibitors described herein, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, may also be prepared from compositions described herein and one or more pharmaceutically acceptable excipients suitable for sublingual, buccal, rectal, intraosseous, intraocular, intranasal, epidural, or intraspinal administration. Preparations for such pharmaceutical compositions are well-known in the art. See, e.g., Anderson, Philip O.; Knoben, James E.; Troutman, William G, eds., Handbook of Clinical Drug Data, Tenth Edition, McGraw-Hill, 2002; and Pratt and Taylor, eds., Principles of Drug Action, Third Edition, Churchill Livingston, N.Y., 1990, each of which is incorporated by reference herein in its entirety. 
     Administration of an active pharmaceutical ingredient or combination of active pharmaceutical ingredients or a pharmaceutical composition thereof can be effected by any method that enables delivery of the compounds to the site of action. These methods include oral routes, intraduodenal routes, parenteral injection (including intravenous, intraarterial, subcutaneous, intramuscular, intravascular, intraperitoneal or infusion), topical (e.g., transdermal application), rectal administration, via local delivery by catheter or stent or through inhalation. The active pharmaceutical ingredient or combination of active pharmaceutical ingredients can also be administered intraadiposally or intrathecally. 
     Exemplary parenteral administration forms include solutions or suspensions of active compound in sterile aqueous solutions, for example, aqueous propylene glycol or dextrose solutions. Such dosage forms can be suitably buffered, if desired. 
     Kits 
     The disclosure also provides kits. The kits include an active pharmaceutical ingredient or combination of active pharmaceutical ingredients, either alone or in combination in suitable packaging, and written material that can include instructions for use, discussion of clinical studies and listing of side effects. Such kits may also include information, such as scientific literature references, package insert materials, clinical trial results, and/or summaries of these and the like, which indicate or establish the activities and/or advantages of the composition, and/or which describe dosing, administration, side effects, drug interactions, or other information useful to the health care provider. Such information may be based on the results of various studies, for example, studies using experimental animals involving in vivo models and studies based on human clinical trials. The kit may further contain another active pharmaceutical ingredient. In selected embodiments, an active pharmaceutical ingredient or combination of active pharmaceutical ingredients are provided as separate compositions in separate containers within the kit. In selected embodiments, an active pharmaceutical ingredient or combination of active pharmaceutical ingredients are provided as a single composition within a container in the kit. Suitable packaging and additional articles for use (e.g., measuring cup for liquid preparations, foil wrapping to minimize exposure to air, and the like) are known in the art and may be included in the kit. Kits described herein can be provided, marketed and/or promoted to health providers, including physicians, nurses, pharmacists, formulary officials, and the like. Kits may also, in selected embodiments, be marketed directly to the consumer. 
     In some embodiments, the disclosure provides a kit comprising a composition comprising a therapeutically effective amount of an active pharmaceutical ingredient (e.g., a p38α MAPK inhibitor, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, or combination of active pharmaceutical ingredients or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof. These compositions are typically pharmaceutical compositions. The kit is for co-administration of the active pharmaceutical ingredient or combination of active pharmaceutical ingredients, either simultaneously or separately. 
     In some embodiments, the disclosure provides a kit comprising (1) a composition comprising a therapeutically effective amount of an active pharmaceutical ingredient (e.g., a p38α MAPK inhibitor, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, or combination of active pharmaceutical ingredients or a pharmaceutically acceptable salt, solvate, hydrate, cocrystal, or prodrug thereof, and (2) a diagnostic test for determining whether a patient&#39;s cancer is a particular subtype of a cancer. Any of the foregoing diagnostic methods may be utilized in the kit. 
     The kits described above are preferably for use in the treatment of the diseases and conditions described herein. In some embodiments, the kits are for use in the treatment of an inflammatory disease. In some embodiments, the kits are for use in the treatment of rheumatoid arthritis, a cardiovascular disease, multiple sclerosis, inflammatory bowel disease, chronic obstructive pulmonary disease (COPD), asthma, acute respiratory distress syndrome (ARDS), or acute lung injury (ALI). In a particular embodiment, the kits are for use in the treatment of hyperproliferative disorders, such as cancer. 
     In a particular embodiment, the kits described herein are for use in the treatment of cancer. In some embodiments, the kits described herein are for use in the treatment of a cancer selected from the group consisting of pancreatic cancer, breast cancer, prostate cancer, lymphoma, skin cancer, colon cancer, melanoma, malignant melanoma, ovarian cancer, brain cancer, primary brain carcinoma, head-neck cancer, glioma, glioblastoma, liver cancer, bladder cancer, non-small cell lung cancer, head or neck carcinoma, breast carcinoma, ovarian carcinoma, lung carcinoma, small-cell lung carcinoma, Wilms&#39; tumor, cervical carcinoma, testicular carcinoma, bladder carcinoma, pancreatic carcinoma, stomach carcinoma, colon carcinoma, prostatic carcinoma, genitourinary carcinoma, thyroid carcinoma, esophageal carcinoma, myeloma, multiple myeloma, adrenal carcinoma, renal cell carcinoma, endometrial carcinoma, adrenal cortex carcinoma, malignant pancreatic insulinoma, malignant carcinoid carcinoma, choriocarcinoma, mycosis fungoides, malignant hypercalcemia, cervical hyperplasia, leukemia, acute lymphocytic leukemia, chronic lymphocytic leukemia, acute myelogenous leukemia, chronic myelogenous leukemia, chronic granulocytic leukemia, acute granulocytic leukemia, hairy cell leukemia, neuroblastoma, rhabdomyosarcoma, Kaposi&#39;s sarcoma, polycythemia vera, essential thrombocytosis, Hodgkin&#39;s disease, non-Hodgkin&#39;s lymphoma, soft-tissue sarcoma, osteogenic sarcoma, primary macroglobulinemia, and retinoblastoma. In particular embodiments, the kits described herein are for use in the treatment of malignant melanoma. 
     Dosages and Dosing Regimens 
     The amounts of the pharmaceutical compositions administered using the methods herein, such as the dosages of p38α MAPK inhibitors, for example dosages of any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, will be dependent on the human or mammal being treated, the severity of the disorder or condition, the rate of administration, the disposition of the active pharmaceutical ingredients and the discretion of the prescribing physician. However, an effective dosage is in the range of about 0.001 to about 100 mg per kg body weight per day, such as about 1 to about 35 mg/kg/day, in single or divided doses. For a 70 kg human, this would amount to about 0.05 to 7 g/day, such as about 0.05 to about 2.5 g/day. In some instances, dosage levels below the lower limit of the aforesaid range may be more than adequate, while in other cases still larger doses may be employed without causing any harmful side effect—e.g., by dividing such larger doses into several small doses for administration throughout the day. The dosage of the pharmaceutical compositions and active pharmaceutical ingredients may be provided in units of mg/kg of body mass or in mg/m 2  of body surface area. 
     In some embodiments, the disclosure includes methods of treating a cancer in a human subject suffering from the cancer in which cancer cells overexpress p38α MAPK, the method comprising the steps of administering a therapeutically effective dose of an active pharmaceutical ingredient that is a p38α MAPK inhibitor, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, to the human subject. 
     In some embodiments, the disclosure includes methods of treating a cancer in a human subject suffering from the cancer in which cancer cells overexpress p38α MAPK, the method comprising the steps of administering a therapeutically effective dose of an active pharmaceutical ingredient that is a p38α MAPK inhibitor, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, to the human subject to inhibit or decrease the activity of p38α MAPK protein. 
     In some embodiments, a pharmaceutical composition or active pharmaceutical ingredient is administered in a single dose. Such administration may be by injection, e.g., intravenous injection, in order to introduce the active pharmaceutical ingredient quickly. However, other routes, including the preferred oral route, may be used as appropriate. A single dose of a pharmaceutical composition may also be used for treatment of an acute condition. 
     In some embodiments, a pharmaceutical composition or active pharmaceutical ingredient is administered in multiple doses. In an embodiment, a pharmaceutical composition is administered in multiple doses. Dosing may be once, twice, three times, four times, five times, six times, or more than six times per day. Dosing may be once a month, once every two weeks, once a week, or once every other day. In other embodiments, a pharmaceutical composition is administered about once per day to about 6 times per day. In some embodiments, a pharmaceutical composition is administered once daily, while in other embodiments, a pharmaceutical composition is administered twice daily, and in other embodiments a pharmaceutical composition is administered three times daily. 
     Administration of the active pharmaceutical ingredients may continue as long as necessary. In selected embodiments, a pharmaceutical composition is administered for more than 1, 2, 3, 4, 5, 6, 7, 14, or 28 day(s). In some embodiments, a pharmaceutical composition is administered for less than 28, 14, 7, 6, 5, 4, 3, 2, or 1 day(s). In some embodiments, a pharmaceutical composition is administered chronically on an ongoing basis—e.g., for the treatment of chronic effects. In some embodiments, the administration of a pharmaceutical composition continues for less than about 7 days. In yet another embodiment the administration continues for more than about 6, 10, 14, 28 days, two months, six months, or one year. In some cases, continuous dosing is achieved and maintained as long as necessary. 
     In some embodiments, an effective dosage of an active pharmaceutical ingredient disclosed herein, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, is in the range of about 1 mg to about 500 mg, about 10 mg to about 300 mg, about 20 mg to about 250 mg, about 25 mg to about 200 mg, about 10 mg to about 200 mg, about 20 mg to about 150 mg, about 30 mg to about 120 mg, about 10 mg to about 90 mg, about 20 mg to about 80 mg, about 30 mg to about 70 mg, about 40 mg to about 60 mg, about 45 mg to about 55 mg, about 48 mg to about 52 mg, about 50 mg to about 150 mg, about 60 mg to about 140 mg, about 70 mg to about 130 mg, about 80 mg to about 120 mg, about 90 mg to about 110 mg, about 95 mg to about 105 mg, about 150 mg to about 250 mg, about 160 mg to about 240 mg, about 170 mg to about 230 mg, about 180 mg to about 220 mg, about 190 mg to about 210 mg, about 195 mg to about 205 mg, or about 198 to about 202 mg. In some embodiments, an effective dosage of an active pharmaceutical ingredient disclosed herein is less than about 25 mg, less than about 50 mg, less than about 75 mg, less than about 100 mg, less than about 125 mg, less than about 150 mg, less than about 175 mg, less than about 200 mg, less than about 225 mg, or less than about 250 mg. In some embodiments, an effective dosage of an active pharmaceutical ingredient disclosed herein is greater than about 25 mg, greater than about 50 mg, greater than about 75 mg, greater than about 100 mg, greater than about 125 mg, greater than about 150 mg, greater than about 175 mg, greater than about 200 mg, greater than about 225 mg, or greater than about 250 mg. 
     In some embodiments, an effective dosage of an active pharmaceutical ingredient disclosed herein, for example any of the compounds of any of formulas A, B, I, II, I-a to XIII-a, 1001-1180 (e.g., formulas 1001 (SF-6-221), 1032 (SF-7-008), 1034 (SF-7-010), 1035 (SF-7-011), 1036 (SF-7-012), 1037 (SF-6-217), 1043 (SF-6-223), 1049 (SF-6-224), 1061 (SF-6-219), 1085 (SF-6-222), SF-7-009, and 1087 (SF-7-044)), formulas 2001-2020, is in the range of about 0.01 mg/kg to about 200 mg/kg, or about 0.1 to 100 mg/kg, or about 1 to 50 mg/kg. 
     In some embodiments, an active pharmaceutical ingredient is administered at a dosage of 10 to 200 mg BID, including 50, 60, 70, 80, 90, 100, 150, or 200 mg BID. In some embodiments, an active pharmaceutical ingredient is administered at a dosage of 10 to 500 mg BID, including 1, 5, 10, 15, 25, 50, 75, 100, 150, 200, 300, 400, or 500 mg BID. 
     In some instances, dosage levels below the lower limit of the aforesaid ranges may be more than adequate, while in other cases still larger doses may be employed without causing any harmful side effect, e.g., by dividing such larger doses into several small doses for administration throughout the day. Of course, as those skilled in the art will appreciate, the dosage actually administered will depend upon the condition being treated, the age, health and weight of the recipient, the type of concurrent treatment, if any, and the frequency of treatment. Moreover, the effective dosage amount may be determined by one skilled in the art on the basis of routine empirical activity testing to measure the bioactivity of the compound(s) in a bioassay, and thus establish the appropriate dosage to be administered. 
     An effective amount of the combination of the active pharmaceutical ingredient may be administered in either single or multiple doses by any of the accepted modes of administration of agents having similar utilities, including rectal, buccal, intranasal and transdermal routes, by intra-arterial injection, intravenously, intraperitoneally, parenterally, intramuscularly, subcutaneously, orally, topically, or as an inhalant. 
     In some embodiments, the compositions described herein further include controlled-release, sustained release, or extended-release therapeutic dosage forms for administration of the compounds described herein, which involves incorporation of the compounds into a suitable delivery system in the formation of certain compositions. This dosage form controls release of the compound(s) in such a manner that an effective concentration of the compound(s) in the bloodstream may be maintained over an extended period of time, with the concentration in the blood remaining relatively constant, to improve therapeutic results and/or minimize side effects. Additionally, a controlled-release system would provide minimum peak to trough fluctuations in blood plasma levels of the compound. 
     The following examples describe the disclosure in further detail. These examples are provided for illustrative purposes only, and should in no way be considered as limiting the disclosure. 
     EXAMPLES 
     Materials and Methods 
     Chemicals, recombinant proteins and antibodies: Mouse anti-human p38α and rabbit anti-phospho-MK2 (T222) and phospho-Stat-1 (S727) were purchased from Cell Signaling Technology (Danvers, Mass.). The coding sequences for human p38α variant 2 and p38β (with N-terminal HA tag) were amplified by PCR and cloned into pRSetA (Thermo Fisher). Mutations were introduced into p38α using Quikchange (Stratagene) and confirmed by bidirectional sequencing. Plasmids were transformed in  E. coli  BL21 and proteins were purified using cobalt columns (TALON™; Clontech Laboratories; Mountain View, Calif.) and confirmed by SDS-PAGE and Western blotting. The compounds identified in the CADD screen were purchased from Maybridge Chemical Co. (Belgium). 
     CADD identification of lead compounds ( FIG.  1   d   ): Based on the X-ray crystal structure of mouse p38α/MAPK14 (PDB ID: 1P38), a step-wise iterative CADD process was used to screen an in silico database of small molecule compounds available from Maybridge Chemical Screening Collection for the potential to bind in a pocket near the ED substrate binding site ( FIG.  1   a    and  FIG.  1   b   ). In silico preparation of p38α conformation was performed using CHARMM36 and general (CGenFF) force field with the Nanoscale Molecular Dynamics (NAMD) program, to identify local potential ligand-binding pockets. Protein structures were subjected to clustering to identify 20 representative protein conformations to account for protein flexibility. Screening was performed in the following stages: (1) potential inhibitor binding sites were identified; (2) compounds were ranked based on their van der Waals (VDW) and electrostatic interaction energies with the protein binding pockets using the program DOCK with size-based score normalization; (3) the top 50,000 compounds were subjected to a second in silico screen with additional relaxation of the ligands during simulated binding and the top 1,000 compounds were selected based on total interaction energy including score normalization based on ligand size; (4) chemical fingerprint-based cluster analysis of the top scoring compounds using the program MOE (Chemical Computing Group) was performed to identify chemically diverse compounds and the final list of potential p38α-interacting compounds were selected based on a scalar bioavailability metric, 4DBA, that accounts for the physiochemical descriptors in Lipinski&#39;s Rule of Five. 
     Mouse unphosphorylated p38α/MAPK14 variant-1 differs from its human variant-2 by only two amino acids, H48L and A263T and from mouse variant-2 and human variant-1 by only 14 amino acids between residues 230 and 254. Neither these amino differences nor the phosphorylation state of p38α ( FIG.  1   c   ) are predicted to significantly alter the structure of the CD or ED sites or our CADD targeted, thereby validating the use of mouse unphosphorylated p38α variant-1 for the CADD search and unphosphorylated recombinant human p38α variant-2 protein for the DSF screen. 
     Site Identification by Ligand Competitive Saturation (SILCS): An in silico map of all potential ligand-binding pockets in p38α has been completed, including the ED site target, using the Site Identification by Ligand Competitive Saturation (SILCS) method ( FIG.  6   , potential binding sites in green). The SILCS method creates a free energy map (grid free energy; GFE FragMaps) of the functional group interaction pattern of p38α that allows for identification of putative binding sites and rapid free energy estimates of ligand binding to the various p38α sites (ligand GFE or LGFE). The SILCS GFE FragMaps account for protein flexibility, protein desolvation, functional group desolvation, as well as functional group-protein interactions, thereby yielding highly accurate mapping of the protein for use in database screen and lead compound optimization. Each stepwise in silico CADD screen of any compound database starts with the SILCS pharmacophore approach, which takes into account protein flexibility. The secondary screen is based on the MC SILCS approach from which relative free energies of binding are calculated. A final screen based on chemical diversity, physiochemical properties that maximize absorption, distribution, metabolism, and excretion (ADME) characteristics and potential for chemical optimization, generates a list of compounds for testing of selective p38α binding and biological activity. Additional rounds of screening are performed using CADD strategies modified based on the proteomic and structural analyses of lead compounds. Searches of the database for structural analogs of lead compounds from earlier rounds are performed using the program MOE (Chemical Computing Group). 
     Alternative CADD methodology: The program Dock can be used with scoring based on the Dock van der Waals (vdW) interaction energy normalized for molecular weight (MW). This method identifies compounds that sterically fit the binding site while biasing towards low MW compounds. Additional ranking of compounds use generalized linear response methods and include free energy of solvation based on the implicit solvent Generalized Born (GB) model. 
     Alternative p38α targets: the search strategy can be changed to target the CD, or the DEF sites. Since formation of the DEF pocket requires p38α activation, dual-phosphorylated p38α is used for its DSF screen. 
     Differential Scanning Fluorimetry (DSF): Binding of CADD selected compounds to p38α and β isoforms was tested experimentally using DSF, which evaluates changes in the target protein melting temperature (ΔTm) due to interactions with test compound. SYPRO orange (Invitrogen; diluted 1:1000 in 10 mM HEPES, 150 mM NaCl, pH 7.5) and 1 μM unphosphorylated recombinant human p38α were added to 96-well PCR plates, then 50 nM to 200 μM test compound in 100% DMSO (2% final DMSO concentration) was added, the plates mixed, sealed, centrifuged at 1000 rpm for 1 min, and melting curve performed using an Applied Biosystems real time PCR instrument. The melting point was determined from the first derivative curve. In addition, p38β, or target-disrupted p38α mutant are used as well. 
     Although DSF is less sensitive than other assays of ligand:protein binding, it is low-cost and has relatively high throughput. DSF detected p38α binding by 25% of the CADD-identified compounds screened and selective p38α binding by 10%, demonstrating good efficiency of both the CADD and DSF screening strategies. The 10% hit rate of the CADD search for substrate-selective p38α inhibitors was similar to the search for substrate-selective ERK inhibitors, and much greater than the usual 0.1-0.01% hit rate using experimental screening alone. 
     Cell culture: HMVECLs were purchased from Promocell (Heidelberg, Del.), maintained in Endothelial Cell Growth Medium MV2, used at passage 3 to 10, and studied at postconfluence according to the supplier&#39;s protocol. The THP1 human monocyte cell line (American Type Culture Collection/ATCC no. TIB202) was maintained in RPMI 1640 supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES buffer, pH 7.3, penicillin, streptomycin, 0.05 mM β-mercaptoethanol and 10% defined fetal bovine serum (FBS; Gibco, Life Technologies, Grand Island, N.Y.). HeLa cells (ATCC no. CCL-2) were cultured in DMEM with 4.5 g/L glucose, 1 mM sodium pyruvate, 2 mM L-glutamine, penicillin, streptomycin, and 10% FBS. Prior to experimental exposures, THP1 cells were differentiated by treating with 5 ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 24 h, washing with PBS, and culturing at 37° C. in PMA-free media for an additional 24 h. 
     Endothelial permeability assay: Permeability of HMVECL monolayers was assessed by measuring transendothelial flux of 10 kDa dextran conjugated to Cascade blue fluorescent dye for 30 min at 37° C. in Matrigel-coated 3 μm pore size Transwell plates. 
     Cells are treated with test compound at 1-100 M, 10 μM SB203580, or DMSO for 1 h, then with 10 ng/ml rhTNFα at 39.5° C. for 6 h and permeability is assessed by adding 100 μg/ml Cascade-blue-conjugated 10 kDa dextran to the bottom well for 30 min at 37° C. and analyzing fluorescence (400/420 nm) in the upper well. 
     Neutrophil transendothelial migration (TEM) assay: Neutrophils were isolated from heparinized venous blood that was collected from healthy volunteers using a protocol approved by the University of Maryland Institutional Review Board and TEM of calcein-labeled neutrophils through HMVECLs was measured. 
     Cytotoxicity of HMVECL exposed to 10-100 μM of each compound is analyzed by MTS assay (Promega), LDH release (Promega), and immunoblotting for activated caspase-3 (Cell Signaling). 
     Macrophage cytokine expression: The capacity of test compounds to block LPS-induced cytokine expression is assessed in PMA-differentiated THP1 cells using qRT-PCR and Luminex-based immunoassays (UMB Cytokine Core Lab). THP1 cells differentiated with 5 ng/ml PMA for 24 h, are treated with 1-100 μM test compound, 10 μM SB203580, or DMSO for 1 h, then with 100 ng/ml ultrapure  E. coli  0111:B4 LPS (InvivoGen) for 3 h (qRT-PCR; Real Time Primers) or 24 h (supernatants for immunoassays). 
     Mouse acute lung injury Model: Male CD-1 mice weighing 25-30 g were purchased from Charles River and housed in the Baltimore Veterans Administration Medical Center Animal Care Facility under AALAC-approved conditions. All protocols were approved by the University of Maryland Baltimore IACUC. Inhibitors were tested in a mouse i.t. LPS/FRH-induced ALI model. Mice were pretreated with SB203580 or putative p38 inhibitors in ≤2% DMSO via 0.5 ml i.p. injection 1 h prior to i.t. instillation of 50 μg LPS and switch to a 37° C. incubator, which increases core temperature to −39.5° C. Mice were euthanized after 24 h, the lungs lavaged with a total of 2 ml PBS, the cells counted and the cell free lavage fluid analyzed for protein content using the Bradford method (Biorad). 
     Animals are anesthetized with inhaled isoflurane during surgery to implant intraperitoneal thermistors. Mice receive 0.05-0.1 mg/kg buprenorphine analgesia s.c. Q12 h for 2 postoperative days. If significant distress occurs during the ALI model buprenorphine analgesia is administered. LPS is administered in 50 μl PBS via instillation in the posterior oropharynx during anesthesia with isoflurane. p38 inhibitors are administered via i.p. injection with a 25 g needle with the mouse conscious and lightly restrained. 
     The combination of FRH and intratracheal LPS induces robust pulmonary neutrophil influx, cytokine expression, and protein leak by 12-24 h and 50% mortality beginning at 48 h. UM101 was more potent than SB203580 in reducing neutrophil and protein accumulation in BAL in this model. Thus, to minimize the number of mice required for this screen, lung injury, lung and extrapulmonary inflammation, and drug toxicity are measured at a single 24 h time point, including BAL protein, neutrophil, and proinflammatory cytokine content, serum levels of IL-6, creatinine and AST (Abcam), and Cardiac Troponin I (MyBiosource). Novel compounds are tested at doses of 4, 12, and 40 mg/kg and compared with vehicle (DMSO)- and SB203580 (40 mg/kg)-treated controls. All vehicle- and drug-treated mice are exposed to i.t. LPS/FRH and compared with naïve mice. 4 mice per group can be used. 
     Generally, screening is done in prevention models and final candidates are also evaluated in a treatment model. 
     Inhibition of substrate phosphorylation: A functional analysis of UM101 to block p38-dependent phosphorylation of MK2 and Stat-1 was performed in HeLa cells. The cells were pre-treated with SB203580 or UM101 for 30 min and then activated with 10 μM anisomycin for 30 min. Cell extracts prepared in RIPA buffer containing protease and phosphatase inhibitors were resolved by SDS-PAGE, transferred to PVDF membrane, blocked with 5% nonfat dry milk, probed with primary antibodies against phosphorylated MK2 and Stat-1, and total p38α as a loading control. Bands were detected using secondary antibodies conjugated to infrared fluorophores and infrared fluorescence imaging (Odyssey; LICOR). 
     Cytotoxicity assay: Cytotoxicity was monitored in parallel HMVEC-L monolayers established in 96-well culture plates using a colorimetric assay that measured reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) to a formazan dye (CellTiter 96™; Promega; Madison, Wis.) according to the manufacturer&#39;s protocol and quantifying product formation by measuring absorption at 490 nm. 
     Gene Expression: RNA integrity was confirmed by Agilent Bioanalyzer 2100 and all samples were confirmed to have RNA integrity scores (RIN) of 10 prior to further analysis. Poly(A)-enriched samples were reverse transcribed and sequenced using the Illumina HiSeq platform to generate at least 90 million reads per sample. Intergenic sequence accounted for less than 0.7% of all reads indicating minimal genomic DNA contamination. Raw data were analyzed using the TopHat read alignment tool and  Homo sapiens  genomic reference sequence (Ensembl version GRCh38.78). Differential gene expression was analyzed using the DESeq R package (Bioconductor) and the negative binomial model. Criteria for significant differences in gene expression were (1) false discovery rate (FDR) &lt;0.05, (2) expression level &gt;10 th  percentile, and (3) ≥2-fold change. The differential gene expression patterns were further analyzed using the PathwayNet (Troyanskaya Lab, Princeton) and Ingenuity™ Pathway Analysis (Qiagen) tools. Cytokine gene expression in THP1 cells was analyzed by quantitative RT-PCR using primers in a commercially available PCR array (HCA-II array; Real Time Primers; Elkins Park, Pa.) and SYBR-green reaction mix (Biorad), and a BioRad iCycler IQ Optical Module according to the supplier&#39;s protocol. Data were quantified using the Gene Expression Ct Difference method, and standardized to levels of the housekeeping gene, GAPDH, using Ct values automatically determined by the thermocycler. 
     Saturation Transfer Difference Nuclear Magnetic Resonance (STD-NMR): A 40 mM stock solution of UM101 was made in d6-DMSO. STD-NMR samples contained 150 mM NaCl, 50 mM phosphate, pH 7, 200 μM UM101, and 5 μM p38 protein in D20. Spectra were recorded on an Agilent DD2 500-MHz spectrometer equipped with a 5-mm inverse HFCN probe head at 300 K. During each transient, the protein was saturated with a series of 58 GAUSSIAN-shaped pulses (50 ms, 1 ms delay between pulses) using the vendor-supplied STD-ES pulse sequence, for a total saturation time of 3 seconds. The on-resonance irradiation of protein was done at 0.5 ppm and off-resonance irradiation at 30 ppm. The vendor-supplied WATERGATE pulse sequence was used to suppress the water signal in the STD spectrum. The on-resonance and off-resonance pulse sequences were subtracted internally. A total of 16,384 transients were collected for each STD experiment with a 1 second delay between acquisitions, 6000 Hz spectral width, and 1.3 second acquisition time. 
     Comparative proteome and phosphopeptide expression profiling by mass spectrometry (MS): Protein expression and the percentage of phosphorylation of specific proteins is quantified in a label-free manner using mass spectrometry-based techniques. Specifically, the effects of compounds of the disclosure are compared with SB203580 on protein phosphorylation pattern and proteome expression in TNFα-stimulated HMVECLs and LPS-stimulated THP1 cells using LC-MS/MS. Cells are pretreated for 30 min with 10 μM SB203580 or test compound at EC 50  and EC 90  (based on HMVECL permeability and THP1 IL-8 expression assays). For phosphopeptide analysis, cells are stimulated for 0.5, 1.5, and 4 h. Tryptic phosphopeptides are enriched using a commercially available TiO 2  enrichment protocol (Pierce), then analyzed on a nanoUPLC coupled Thermo Orbitrap Fusion Tribrid Mass Spectrometer using three strategies: (1) hybrid Electron-Transfer (ETD)/Higher-Energy Collision (HCD) Dissociation (EThcD); (2) data-dependent decision tree (DDDT) logic; (3) HCD product-dependent ETD (HCD-pd-ETD); and/or (4) nanoUPLC coupled Waters Synapt G2S Mass Spectrometer, using ion mobility linked parallel MS (UDMSC). For comparative proteome expression analysis, cells are stimulated for 4 and 12 h and lysates analyzed on the nanoUPLC coupled Waters Synapt G2S and/or nanoUPLC coupled Thermo Orbitrap Fusion Tribrid using UDMS E  and ADAPT-DDA, respectively. Relative peptide abundance is measured by comparing the MS1 peak area of peptide ions, whose identities and phosphorylation events are confirmed by MS2 sequencing using the different fragmentation strategies described above (EThcD, DDDT, HCD-pd-ETD and UDMS®). An aligned AMRT (accurate mass and retention time) cluster quantification algorithm as described is used for label free quantification. 
     Immunoblot analysis: Changes in total in vivo proteome and the phosphoproteome are confirmed by immunoblotting using commercial antibodies and infrared fluorescence imaging (Odyssey; LICOR). In vitro kinase assays is performed in reactions containing recombinant active p38α and one or more recombinant substrate proteins and analyzed by immunoblotting with phosphospecific antibodies. 
     X-ray crystallography: Higher resolution analysis of compound binding to p38α is provided by x-ray crystallography. The primary approach to growing p38α includes co-crystallization of compound crystals with a 2:1 compound:p38α molar ratio. Alternatively, compounds are soaked into preformed p38α crystals. Diffraction quality protein crystals are grown and screened using an automated system comprising an Alchemist DT screen maker, Gryphon drop setter with LCP module, and Minstrel DT UV/Vis automated visualization system (Rigaku). These structures are solved by molecular replacement methods using known p38α structures and standard crystallographic analysis software (SBGrid). 
     Analysis of p38-binding kinetics: The KD for the compounds of the disclosure are estimated by DSF. ITC is performed to refine the KD calculation of compounds and produce thermodynamic information to facilitate ligand optimization. Data is collected on an Auto ITC HT Microcalorimeter (MicroCal). Recombinant p38α (10 AM) and stock concentration of test compounds (200 μM) are prepared in identical buffers containing low ionization energy (e.g., 50 mM phosphate or citrate with 50 mM NaCl) and degassed. Heat generation/absorption during titration of compound are measured and analyzed with MicroCal software. 
     Pharmacokinetic/pharmacodynamic (PK/PD) analysis of lead compounds: Compounds are comprehensively analyzed for in vivo toxicity and effectiveness as both prevention and treatment in the intratracheal LPS+FRH-induced mouse ALI model. This model is a short-term model of human ARDS, amenable to parenteral dosing of therapeutic agents, characterized by extensive endothelial permeability, neutrophil accumulation, proinflammatory cytokine and chemokine expression, epithelial injury, and -50% mortality beginning after 48 h. The results are generalizable to other inflammatory diseases. Compounds are solubilized in a final concentration of ≤1% DMSO and administered as a single intraperitoneal injection. Maximal tolerated dose (MTD) is determined by monitoring mice for 24 h for signs of distress (including change in locomotor activity, weight loss, reduced grooming, and ruffled fur), creatinine, BUN, aspartate transaminase (AST), and cardiac troponin. Inhibitors are administered either 30 min prior to or 8 h after LPS+FRH as models of prevention or treatment, respectively. 
     Animal number and sex: All testing is performed in CD1 mice, a robust strain in which ALI and pneumonia models have been validated. Dose escalation uses 2 mice per dose and 24 h observation according to published guidelines. Survival differences are tested in groups of 20 mice (to detect differences in survival of 75% vs. 25%; α=0.05; β=0.2). Group sizes of 6 mice for BAL and plasma analysis of injury/inflammation and lung homogenates for analysis of apoptotic signaling and groups of 4 mice for histology are used. Survival experiments are performed in equal numbers of male and female mice and differences compared by 2-way ANOVA. Additional experiments are added to analyze any unexpected gender differences found in drug effects. In some embodiments, experiments will use male mice. 
     Maximal Tolerated Dose of most potent, structurally distinct compounds is determined by measuring toxicity of 20, 40, and 80 mg/kg i.p. in 2 mice per dose, monitored for 24 h and euthanized. Serum is analyzed for markers of hepatic, renal, and cardiac toxicity. Kidney, heart, liver and lung (inflated) are fixed, paraffin-embedded, H&amp;E-stained, and examined for inflammation and injury. Control mice receive vehicle (1% DMSO). Toxic compounds are replaced by the next structurally distinct compound on the list of candidates. 
     Activity of Inhibitors in blocking FRH augmented LPS-induced ALI: Compounds are tested at the MTD in the LPS+FRH-induced ALI model. 
     Effect of pretreatment on survival: Effectiveness of pretreatment with test compounds at the MTD on survival in LPS/FRH-challenged mice is compared with 40 mg/kg SB203580 and vehicle (1% DMSO) in groups of 20 mice. Mice receive pretreatment as a single 0.5 ml injection and 30 min later receive 50 μg LPS via i.t. instillation and placed in 37° C. ambient temperature. This exposure increases core temperature from 36.5° C. to 39.5° C., but is confirmed in some mice using telemetric temperature monitoring (Data Sciences International; St. Paul, Minn.). Mice are monitored for survival using moribundity as a surrogate for death. Those compounds showing survival advantage vs. DMSO are further analyzed for efficacy when given 24 h post-LPS. Ineffective compounds are replaced by the next compound from the candidate list. Effective compounds are further tested at 10% and 30% of the MTD. 
     Effect of post-LPS dosing on survival: Compounds that are effective as pretreatment are analyzed for effectiveness at the same doses using the same protocol except delaying dosing until 8 h after LPS instillation and initiation of FRH. Compounds conferring survival advantage vs. SB203580 are analyzed for biological effects and PK. Ineffective compounds are replaced by the next compound on the list. 
     Effect of compounds on inflammation, lung injury, and permeability: The most effective compounds in the survival experiments are further analyzed for effects on lung injury and inflammation in the LPS+FRH ALI model. Mice are pretreated with each compound at its ED50 based on the survival experiments, 40 mg/kg SB203580, or DMSO 30 min prior to or 8 h-after LPS/FRH challenge and euthanized 24 h post-LPS. In 6 mice per group, BALF is collected and analyzed for neutrophil content by counting modified-Giemsa-stained cytopreps, total protein by Bradford method, and levels of cytokines by Luminex-based immunoassay (UMB Cytokine Core Lab). After lavage, lungs are excised, snap-frozen in liquid nitrogen and homogenates prepared for immunoblotting of candidate p38α substrates to confirm substrate inhibitor effects found in vitro. Lungs from 4 mice per group are inflation/fixed at 20 cm H 2 O with Prefer™, paraffin embedded, H&amp;E stained, or GR-1 immunostained to analyze lung injury and neutrophil infiltration, and TUNEL staining and immunostaining for active caspase-3 to assess apoptosis. Serum IL-6 is measured as an indicator of systemic inflammation. 
     Pharmacokinetics of novel p38 modifiers: PK of effective compounds in mice is characterized. First, a bioanalytical method for each compound is developed and validated according to FDA Guidance. PK studies are then conducted to determine lung uptake and key PK parameters that characterize each compound (i.e., clearance (CL), volume of distribution (Vd), maximum plasma conc. (C max ), time to reach C max  (T max ), area under the plasma concentration curve (AUC) and half-life (tin)). The PK parameters are used to estimate the time needed to reach steady state plasma concentration (equivalent to five half-lives), and to guide dose selection for further PD studies. In addition, these studies help rank the tested p38 modifiers in terms of their lung/plasma conc. ratios. For each study, CD1 mice (n=30) are treated with a single i.p. dose (10-50 mg/kg) of the selected p38 modifier (dose range for each p38 modifier is dependent on the outcome of studies outlined above. In some embodiments, mice (n=3/time point) are euthanized at a pre-dose and at 5, 15, 30, 60, 120, 240, 360, 600, 720 min post dose. Blood and lung samples are analyzed using validated HPLC methods. 
     Data Analysis: Pathways modified by compounds of the disclosure as compared with SB203580 are deduced—by: (1) analyzing comparative proteome expression using Ingenuity Pathway Analysis and PathwayNet, similarly to RNASeq data from UM101; and (2) analyzing the comparative phosphoproteome by quantitative approaches and bioinformatics. The mass spectrometry results are confirmed by analyzing phosphorylation of candidate substrates in cells and in vitro kinase assays by immunoblotting. Off-target binding suggested by the proteomics data is evaluated over a broad concentration range of test compound by DSF and STD-NMR and by phosphoimmunoblotting for specific substrates and confirmed in in vitro kinase reactions. By identifying common pathways modified by multiple lead compounds and their interactions with p38α, the common p38α effects required for their favorable biological activity are deduced and incorporated into the CADD algorithm for subsequent searches and lead compound optimization. 
     Since the objective of this disclosure is, in one embodiment, to identify and characterize the PD/PK properties of novel anti-inflammatory compounds, these compounds are tested in one embodiment, in order of activity based on functional screens, and compounds that fail toxicity or efficacy in survival studies are replaced with the next most potent and structurally dissimilar compound. Compounds are compared with vehicle alone and SB203580 using one-way ANOVA/Fisher PLSD. The PK data is analyzed by the naive averaging method. Compartmental modeling is used to estimate various pharmacokinetic parameters using Phoenix platform (ver. 1.3, Pharsight, Sunnyvale, Calif.). Several compartmental models are evaluated to determine the best-fit model. A variety of weighting schemes are used including equal weight, 1/y, 1/y{circumflex over ( )}, 1/y 2 , and 1/y{circumflex over ( )} 2 , where y is the observed drug conc., and y{circumflex over ( )} is the model-predicted drug conc. In some embodiments, a final model is selected based on goodness-of-fit plots, weighted residual sum of squares, random distribution of residuals, precision of parameter estimates, Akaike&#39;s information criteria, and Schwarz criteria. After the final model is developed, estimates of the PK parameters are reported including plasma CL, Vd, C max , T max , AUC, and t 1/2 . Lung uptake is represented as a lung/plasma (L/P) conc. ratio. 
     Alternative Approaches: If phosphospecific antibodies are not available and phosphorylation does not cause detectable shifts on immunoblots, cell lysates can be enriched using TiO 2  prior to immunoblotting. Incubation times can be adjusted, as needed, based on in vitro and in vivo proteomics and immunoblot results. Low protein abundance could preclude phosphoprotein detection in cell lysates despite maximum starting material or using isolated cell fractions. In this case, the in vivo cellular phosphoproteome analysis can be augmented by using LC-MS-MS to comprehensively analyze the effects of inhibitors on phosphopeptide patterns in p38α in vitro kinase assays using cell lysates as substrate after inactivating endogenous kinases with 5′-4-fluorosulphonylbenzoyladenosine (FSBA). Stable isotope dimethyl labeling can be used in case of ambiguous label-free results. Other back-up technologies include deuterium-hydrogen exchange mass spectroscopy and NMR, and DSF/STD-NMR-assessed binding to wild-type p38α and CADD-target-mutants. Surface plasmon resonance (SPR) (Biacore T200 Core) can be evaluated as an alternative to ITC to reduce the protein/compound requirements. 
     Statistical methods: Data are presented as mean f SE. Differences among &gt;2 groups were analyzed by applying a Tukey Honestly Significant Difference test to a one-way analysis of variance (ANOVA). Differences between dose-response curves was analyzed by multivariate ANOVA (MANOVA) Differences with p&lt;0.05 were considered significant. 
     Example 1: CADD Modeling of p38 MAPK Substrate-Docking Site, Compound Identification, and Screening Compounds for Direct, Selective Interaction with p38α 
     The inhibitors and methods of the disclosure relate to a CADD-based strategy to identify low molecular weight compounds predicted to bind near the ED substrate-docking site of mouse unphosphorylated p38α (MAPK14 variant-1; PDB:IP38), which is &gt;99% identical with human p38α (variant-2) ( FIG.  1   a   ). The ED and CD sites in p38α are located at either end of a substrate-binding cleft located on the opposite side of the protein from the catalytic site ( FIG.  1   a   ). A pocket near the ED binding site comprising 10 amino acids, only 7 of which were identical in p38α and p38β, was identified ( FIG.  1   b   ). Overlay of structures of mouse unphosphorylated (PDB:IP38) and dual-phosphorylated p38α (PDB:3PY3) revealed near-superimposition of the targeted pocket in the two forms ( FIG.  1   c   ). 
     An overview of the CADD screening and compound testing protocols is shown in  FIG.  1   d   . The compounds in the Maybridge Screening Collection were analyzed for binding to the targeted p38α pocket based on Van Der Waals (VDW) and electrostatic interaction energies, chemical diversity by chemical fingerprint-based cluster analysis, solubility, molecular weight and number of hydrogen bonding functional groups that maximize bioavailability. 
     Twenty structurally dissimilar compounds were selected for functional analysis (Table 2), out of a panel of 150 diverse compounds (Table 3) which were selected for potential biological testing. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 CADD-identified p38α ED site-binding candidates 
               
               
                 screened for p38α binding 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 p38α 
                 ERK2 
               
               
                   
                   
                   
                   
                 ΔTm (° C.) 
                 ΔTm (° C.) 
               
               
                 CADD no. 
                 Compound ID 1   
                 MW 
                 logP 2   
                 @100 μM 3   
                 @100 μM 3   
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 2 
                 SEW 06373 
                 417 
                 3.19 
                 −0.05 
                 0.412 
               
               
                 3 
                 HTS 02798 
                 415 
                 0.67 
                 0.282 
                 0.337 
               
               
                 4 
                 HTS 13333 
                 312 
                 −1.10 
                 0.065 
                 0.452 
               
               
                 5 
                 SCR 00846 
                 418 
                 2.22 
                 0.808 
                 0.628 
               
               
                 8 
                 AW 00509 
                 317 
                 1.13 
                 −0.07 
                 0.531 
               
               
                 13 
                 SEW 06264 
                 309 
                 0.28 
                 0.005 
                 0.390 
               
               
                 16 
                 SCR 00610 
                 339 
                 1.69 
                 −0.052 
                 0.444 
               
               
                 23 
                 SCR 01200 
                 378 
                 2.79 
                 −0.488 
                 −0.598 
               
               
                 29 
                 BTB 05645 
                 350 
                 3.07 
                 −0.353 
                 0.342 
               
               
                 31 
                 KM 04113 
                 304 
                 1.83 
                 −0.278 
                 0.153 
               
               
                 43 
                 CD 11992 
                 300 
                 1.16 
                 −0.485 
                 0.151 
               
               
                 55 
                 SP 01164 
                 2.11 
                 1.92 
                 −0.506 
                 0.022 
               
               
                 60 
                 BTB 13869 
                 426 
                 0.28 
                 0.735 
                 0.195 
               
               
                 63 
                 PD 00612 
                 294 
                 0.61 
                 −0.287 
                 0.075 
               
               
                 69 
                 KM 00081 
                 345 
                 1.68 
                 −0.233 
                 0.361 
               
               
                 101 
                 HTS 05732 
                 378 
                 2.31 
                 0.667 
                 0.0175 
               
               
                 115 
                 NRB 03986 
                 278 
                 3.88 
                 −0.156 
                 0.246 
               
               
                 141 
                 SEW 02182 
                 318 
                 2.46 
                 0.554 
                 0.238 
               
               
                 146 
                 KM 10445 
                 313 
                 2.55 
                 −1.084 
                 −1.632 
               
               
                 150 
                 HTS 03239 
                 341 
                 1.68 
                 −0.171 
                 0.133 
               
               
                   
               
               
                   1 Compound ID from Maybridge portfolio. 
               
               
                   2 logP is the logarithm of the estimated octanol/water partition coefficient, a measure of compound solubility 
               
               
                   3 Change in melting temperature relative to DMSO control in DSF assay 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Top 150 CADD-identified p38α ED site-binding candidates 
               
            
           
           
               
               
               
               
            
               
                 CADD no. 
                 Compound ID 1   
                 MW 
                 logP 2   
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 1 
                 AW 1221 
                 442 
                 3.84 
               
               
                 2 
                 SEW 06373 
                 417 
                 3.19 
               
               
                 3 
                 HTS 02798 
                 415 
                 0.67 
               
               
                 4 
                 HTS 13333 
                 312 
                 −1.10 
               
               
                 5 
                 SCR 00846 
                 418 
                 2.22 
               
               
                 6 
                 HTS 01830 
                 400 
                 4.15 
               
               
                 7 
                 KM11105 
                 409 
                 1.27 
               
               
                 8 
                 AW 00509 
                 317 
                 1.13 
               
               
                 9 
                 SCR 01457 
                 401 
                 2.12 
               
               
                 10 
                 KM 09878 
                 362 
                 2.45 
               
               
                 11 
                 BTB 10384 
                 434 
                 2.32 
               
               
                 12 
                 HTS 03243 
                 419 
                 3.46 
               
               
                 13 
                 SEW 06264 
                 309 
                 0.28 
               
               
                 14 
                 CD 06142 
                 382 
                 3.29 
               
               
                 15 
                 KM 08516 
                 382 
                 2.03 
               
               
                 16 
                 SCR 00610 
                 339 
                 1.69 
               
               
                 17 
                 KM 09250 
                 364 
                 0.87 
               
               
                 18 
                 SCR 01462 
                 344 
                 −0.25 
               
               
                 19 
                 KM 08262 
                 375 
                 1.34 
               
               
                 20 
                 SCR 01164 
                 430 
                 3.44 
               
               
                 21 
                 HTS 05992 
                 360 
                 2.65 
               
               
                 22 
                 CD 00735 
                 390 
                 1.72 
               
               
                 23 
                 SCR 01200 
                 378 
                 2.79 
               
               
                 24 
                 SCR 01160 
                 390 
                 0.69 
               
               
                 25 
                 SCR 00883 
                 398 
                 2.09 
               
               
                 26 
                 AW 01002 
                 331 
                 1.49 
               
               
                 27 
                 KM 10346 
                 339 
                 1.52 
               
               
                 28 
                 KM 09924 
                 374 
                 2.25 
               
               
                 29 
                 BTB 05645 
                 350 
                 3.07 
               
               
                 30 
                 HTS 01722 
                 401 
                 3.5 
               
               
                 31 
                 KM 04113 
                 304 
                 1.83 
               
               
                 32 
                 SCR 00662 
                 338 
                 2.62 
               
               
                 33 
                 RJC 02765 
                 348 
                 1.21 
               
               
                 34 
                 HTS 08093 
                 330 
                 0.50 
               
               
                 35 
                 KM 09335 
                 352 
                 1.08 
               
               
                 36 
                 HTS 06913 
                 355 
                 1.52 
               
               
                 37 
                 KM 07646 
                 296 
                 0.23 
               
               
                 38 
                 KM 06447 
                 355 
                 2.44 
               
               
                 39 
                 HTS 01903 
                 444 
                 2.51 
               
               
                 40 
                 KM 06789 
                 333 
                 1.38 
               
               
                 41 
                 EN 00285 
                 380 
                 2.34 
               
               
                 42 
                 JFD 01748 
                 321 
                 2.74 
               
               
                 43 
                 CD 11992 
                 300 
                 1.16 
               
               
                 44 
                 KM 03098 
                 455 
                 2.56 
               
               
                 45 
                 RJF 01988 
                 450 
                 3.99 
               
               
                 46 
                 RH 00635 
                 402 
                 4.14 
               
               
                 47 
                 GK 02919 
                 363 
                 1.17 
               
               
                 48 
                 KM 02331 
                 451 
                 3.96 
               
               
                 49 
                 GK 01789 
                 360 
                 2.91 
               
               
                 50 
                 GK 03735 
                 376 
                 1.38 
               
               
                 51 
                 HTS 05862 
                 364 
                 1.97 
               
               
                 52 
                 KM 07197 
                 337 
                 0.40 
               
               
                 53 
                 BTB 02067 
                 305 
                 1.94 
               
               
                 54 
                 JFD 01679 
                 357 
                 3.55 
               
               
                 55 
                 SP 01164 
                 2.11 
                 1.92 
               
               
                 56 
                 KM 00730 
                 450 
                 1.92 
               
               
                 57 
                 HTS 03184 
                 407 
                 3.33 
               
               
                 58 
                 HTS 01701 
                 397 
                 4.06 
               
               
                 59 
                 HTS 11459 
                 409 
                 −1.37 
               
               
                 60 
                 BTB 13869 
                 426 
                 0.28 
               
               
                 61 
                 RJC 00192 
                 360 
                 3.85 
               
               
                 62 
                 HTS 06577 
                 367 
                 3.73 
               
               
                 63 
                 PD 00612 
                 294 
                 0.61 
               
               
                 64 
                 HTS 09813 
                 453 
                 2.98 
               
               
                 65 
                 RJC 02517 
                 404 
                 1.93 
               
               
                 66 
                 DP 01615 
                 356 
                 4.00 
               
               
                 67 
                 DP 01320 
                 385 
                 3.74 
               
               
                 68 
                 JFD 01765 
                 352 
                 3.24 
               
               
                 69 
                 KM 00081 
                 345 
                 1.68 
               
               
                 70 
                 RDR 03171 
                 419 
                 2.14 
               
               
                 71 
                 HTS 04127 
                 398 
                 2.82 
               
               
                 72 
                 AW 00409 
                 403 
                 2.36 
               
               
                 73 
                 BTB 06009 
                 413 
                 2.14 
               
               
                 74 
                 KM 10383 
                 443 
                 2.81 
               
               
                 75 
                 HTS 05233 
                 369 
                 0.82 
               
               
                 76 
                 KM 05297 
                 428 
                 0.00 
               
               
                 77 
                 CD 11533 
                 373 
                 3.22 
               
               
                 78 
                 KM 04839 
                 441 
                 3.01 
               
               
                 79 
                 CD 09639 
                 460 
                 3.00 
               
               
                 80 
                 HTS 04160 
                 414 
                 2.73 
               
               
                 81 
                 KM 07794 
                 358 
                 3.70 
               
               
                 82 
                 CD 04864 
                 420 
                 3.51 
               
               
                 83 
                 RDR 02594 
                 397 
                 3.10 
               
               
                 84 
                 DP 01806 
                 435 
                 3.43 
               
               
                 85 
                 HTS 03190 
                 388 
                 3.29 
               
               
                 86 
                 KM 09808 
                 405 
                 3.70 
               
               
                 87 
                 CD 09308 
                 396 
                 2.27 
               
               
                 88 
                 SPB 01817 
                 416 
                 3.99 
               
               
                 89 
                 KM 07150 
                 411 
                 2.05 
               
               
                 90 
                 KM 09339 
                 381 
                 0.91 
               
               
                 91 
                 RDR 01132 
                 415 
                 3.32 
               
               
                 92 
                 SS 00046 
                 322 
                 3.63 
               
               
                 93 
                 HTS 02914 
                 351 
                 1.98 
               
               
                 94 
                 KM 02270 
                 381 
                 4.08 
               
               
                 95 
                 CD 09636 
                 366 
                 1.15 
               
               
                 96 
                 KBK 00012 
                 364 
                 3.69 
               
               
                 97 
                 HTS 13527 
                 337 
                 0.78 
               
               
                 98 
                 BB 06821 
                 389 
                 3.99 
               
               
                 99 
                 AW 01218 
                 343 
                 2.37 
               
               
                 100 
                 PD 00703 
                 303 
                 0.33 
               
               
                 101 
                 HTS 05732 
                 378 
                 2.31 
               
               
                 102 
                 HTS 03187 
                 357 
                 0.79 
               
               
                 103 
                 HTS 05493 
                 427 
                 1.73 
               
               
                 104 
                 RJF 01945 
                 356 
                 3.81 
               
               
                 105 
                 CD 05416 
                 378 
                 3.30 
               
               
                 106 
                 CD 08365 
                 285 
                 1.37 
               
               
                 107 
                 SPB 02947 
                 372 
                 3.15 
               
               
                 108 
                 SCR 01004 
                 357 
                 0.95 
               
               
                 109 
                 HTS 05491 
                 429 
                 3.03 
               
               
                 110 
                 HTS 02224 
                 372 
                 0.33 
               
               
                 111 
                 KM 05869 
                 421 
                 1.45 
               
               
                 112 
                 KM 02112 
                 388 
                 3.19 
               
               
                 113 
                 KM 07452 
                 347 
                 0.49 
               
               
                 114 
                 RJC 02844 
                 302 
                 2.65 
               
               
                 115 
                 NRB 03986 
                 278 
                 3.88 
               
               
                 116 
                 SEW 06625 
                 373 
                 3.05 
               
               
                 117 
                 SCR 0170 
                 320 
                 −0.70 
               
               
                 118 
                 SPB 06098 
                 373 
                 4.07 
               
               
                 119 
                 FM 00079 
                 382 
                 3.19 
               
               
                 120 
                 BTB 03095 
                 350 
                 1.91 
               
               
                 121 
                 KM 08272 
                 382 
                 1.99 
               
               
                 122 
                 BTB 07326 
                 458 
                 3.97 
               
               
                 123 
                 HTS 10719 
                 386 
                 3.71 
               
               
                 124 
                 JFD 01751 
                 375 
                 1.21 
               
               
                 125 
                 HTS 05737 
                 366 
                 0.34 
               
               
                 126 
                 BTB 02557 
                 300 
                 −0.17 
               
               
                 127 
                 KM 01947 
                 386 
                 3.26 
               
               
                 128 
                 KM 04674 
                 340 
                 2.99 
               
               
                 129 
                 BTB 14836 
                 358 
                 1.88 
               
               
                 130 
                 KM 07275 
                 346 
                 3.43 
               
               
                 131 
                 RH 02254 
                 321 
                 1.27 
               
               
                 132 
                 S 07734 
                 274 
                 2.06 
               
               
                 133 
                 KM 03963 
                 308 
                 2.90 
               
               
                 134 
                 KM 01163 
                 377 
                 2.95 
               
               
                 135 
                 SEW 05535 
                 324 
                 −1.08 
               
               
                 136 
                 RDR 02622 
                 321 
                 2.97 
               
               
                 137 
                 AW 00695 
                 338 
                 −0.37 
               
               
                 138 
                 RJC 03556 
                 323 
                 1.30 
               
               
                 139 
                 SP 00787 
                 415 
                 2.74 
               
               
                 140 
                 JFD 02020 
                 322 
                 0.84 
               
               
                 141 
                 SEW 02182 
                 318 
                 2.46 
               
               
                 142 
                 SEW 00427 
                 350 
                 1.64 
               
               
                 143 
                 HTS 00966 
                 311 
                 3.43 
               
               
                 144 
                 HTS 02841 
                 339 
                 −0.16 
               
               
                 145 
                 KM 06585 
                 371 
                 2.32 
               
               
                 146 
                 KM 10445 
                 313 
                 2.55 
               
               
                 147 
                 KM 03965 
                 356 
                 3.97 
               
               
                 148 
                 AW 00554 
                 345 
                 0.36 
               
               
                 149 
                 HTS 01470 
                 371 
                 2.01 
               
               
                 150 
                 HTS 03239 
                 341 
                 1.68 
               
               
                   
               
               
                   1 Compound ID from Maybridge portfolio. 
               
               
                   2 logP is the logarithm of the octanol/water partition coefficient, a measure of drug solubility. 
               
            
           
         
       
     
     Test compounds at 10-100 μM were screened for binding to recombinant p38α and ERK2 using DSF ( FIG.  1   e   , Table 1). Five compounds caused concentration-dependent stabilization of p38α, indicating binding. Three of these also stabilized ERK2 (3, 5, and 141 highlighted yellow (with “*”)) and two (highlighted blue (with “+”)), UM60 (N2,N7-di(2-hydroxyethyl)-9-oxo-9H-2,7-fluorenedisulfonamiide) and UM101 (4-chloro-N-{4-[(1,1-dioxo-1lambda˜6˜-,4thiazinan-4-yl)methyl]phenyl}benzaniide), stabilized p38α but not ERK2. These two structurally dissimilar compounds ( FIG.  10   ), added at 100 μM increased the melting temperature of p38α by ˜0.7° C., compared with a 6° C. increase with SB203580. 
     MC SILCS docking of UM101 to the ED site and GFE FragMap analysis has identified several structural features that can be modified to improve selectivity and potency ( FIG.  7   ). The modifiable sites on UM101 correspond to those identified as interacting with the p38α in the NMR STD analysis ( FIG.  3   f - 3   k   ). 
     Example 2: Effects of Compounds on Endothelial Barrier Functions 
     The capacity of UM60 and UM101 to stabilize endothelial barriers to macromolecules and neutrophils in TNFα- and hyperthermia-stressed HMVECL monolayers was tested ( FIG.  2   ). Combined exposure to 1 ng/ml TNFα and hyperthermia (39.5° C.) for 6 h increased permeability for 10 kDa dextran 2.8-fold, compared with untreated 37° C. cells. Pretreating with 10 μM SB203580 for 30 min reduced TNFα/hyperthermia-induced permeability by 50% ( FIG.  2   a   ). Pretreatment with UM60 at 10 and 25 μM had no effect on permeability, but 100 μM UM60 reduced the TNFα/hyperthermia-induced permeability increase by 71% while UM101 at 10, 25, and 100 μM reduced the TNFα/hyperthermia-induced permeability increase by 74%, 89% and &gt;100%, respectively. 
     Preincubating HMVECLs at 39.5° C. for 6 h increased subsequent IL-8-directed neutrophil TEM from 22.8±0.45×10 3  to 31.8±0.54×10 3  neutrophils ( FIG.  2   b   ). Pretreatment with 10 μM SB203580 reduced hyperthermia-augmented neutrophil TEM by 84%. UM60 at 10 and 25 μM and UM101 at 10 μM reduced hyperthermia-augmented increase in TEM by 18%, 89%, and 95%. UM60 at 50 μM and UM101 at 25 and 50 μM reduced TEM to less than baseline levels. Neither compound was toxic in LDH release and MTS assays when added to HMVECLs at 100 μM for 48 h. 
     Example 3: Comparing Effectiveness of SB203580 and UM101 in Mouse ALI 
     The effectiveness of UM60, UM101, and SB203580 in mitigating transalveolar protein and neutrophil extravasation in a mouse model of LPS/hyperthermia-induced ALI was compared ( FIG.  2   c    and  FIG.  2   d   ). Mice received a single intraperitoneal injection of 100, 300, 500, or 1000 μg UM101, 1000 μg UM60, or 1000 μg SB203580 in 0.5 ml 2% DMSO 30 min prior to intratracheal instillation of 50 μg LPS and transfer to hyperthermic chambers. Control mice received DMSO. Four of six UM60-treated, one of six SB203580-treated, and one of eleven DMSO-treated control mice died within 24 h. All sixteen UM101-pretreated mice survived. Lung lavage from DMSO-pretreated, LPS/hyperthermia-challenged mice contained 1.09±0.19 mg/ml protein and 3.97±1.07×10 6  neutrophils. Compared with DMSO-treated controls, lavage protein concentration and neutrophil content in mice pretreated with 1000 μg SB203580 were reduced by 42% and 46.8%, respectively. Lavage protein concentration in mice pretreated with 100 μg, 300 μg, 500 μg, and 1000 μg UM101 was reduced by 0, 44.1%, 43.9%, and 92.9%, respectively and lavage neutrophil content was reduced by 44.4%, 49.5%, 55.3 and 54%, respectively. 
     Example 4: Effect of SB203580 and UM101 on LPS-Induced Gene Expression in Human THP1 Promonocytes 
     The effects of UM101 and SB203580 on inflammatory cytokine expression were compared by pretreating PMA-differentiated THP1 cells with 25 μM SB203580 or 10, 25, or 100 μM UM101 for 30 min, then stimulating with 100 ng/ml LPS, and harvesting RNA 4 h later for analysis by PCR-based cytokine array. Of 16 LPS-stimulated genes in the array, SB203580 inhibited expression of seven, IL-1α, IL-8, TNFSF8 (CD30 ligand), TNFSF9 (CD137 ligand), CXCL5, CCL7, and CCL17 (Table 4). UM101 inhibited expression of all SB203580-inhibited genes except TNFSF9, and inhibited four SB203580-insensitive genes, IL-1β, CXCL1, TNFSF15, and CCL5. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Effects of SB203580 and UM101 on LPS-induced cytokine expression in THP1 cells 1   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                 SB203580 
                 P vs. 
                 UM101 
                 P vs. 
                 UM101 
                 P vs. 
                 UM101 
                 P vs. 
               
               
                 Gene 
                 DMSO 2   
                 ANOVA 3   
                 25 μM 
                 LPS 4   
                 10 μM 
                 LPS 4   
                 25 μM 
                 LPS 
                 100 μM 
                 LPS 4   
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
            
               
                 IL-1A 
                 453 ± 24 
                 &lt;0.0001 
                  141 ± 9.2 
                 &lt;0.0001 
                     424 ± 22.6 
                 0.74 
                  339 ± 13.5 
                 0.041 
                     88 ± 3.33 
                 &lt;0.0001 
               
               
                 IL-8 
                 56.5 ± 3.3 
                 0.0026 
                  9.6 ± 0.1 
                 0.002 
                 35.6 ± 0.7 
                 0.40 
                 26.7 ± 4.1  
                 0.564 
                 19.7 ± 1.8  
                 0.015 
               
               
                 TNFSF8 
                 60.5 ± 5.5 
                 0.0073 
                 20.6 ± 8.8 
                 0.024 
                 23.5 ± 8.3 
                 0.37 
                 10.5 ± 3.9  
                 0.006 
                 20.7 ± 9.5  
                 0.025 
               
               
                 CXCL5 
                 49.7 ± 2.9 
                 &lt;0.0001 
                  3.2 ± 1.0 
                 &lt;0.0001 
                 23.2 ± 3.7 
                 0.0002 
                 8.7 ± 2.9 
                 &lt;0.0001 
                 3.1 ± 0.2 
                 &lt;0.0001 
               
               
                 CCL7 
                 12.8 ± 1.2 
                 &lt;0.0001 
                  4.2 ± 0.3 
                 &lt;0.0001 
                  7.7 ± 0.3 
                 0.0036 
                 6.2 ± 0.9 
                 0.0036 
                     4 ± 0.4 
                 &lt;0.0001 
               
               
                 CCL17 
                 56.9 ± 6.1 
                 &lt;0.0001 
                 21.5 ± 3.7 
                 0.001 
                 30.4 ± 4.7 
                 0.008 
                  11 ± 1.0 
                 0.0004 
                  2.5 ± 0.33 
                 &lt;0.0001 
               
               
                 TNFSF9 
                 50.8 ± 6.1 
                 0.0046 
                 20.7 ± 3.1 
                 0.0054 
                     48 ± 2.1 
                 0.99 
                 38.2 ± 6.9  
                 0.334 
                     32 ± 1.12 
                 0.086 
               
               
                 IL-1B 
                  171 ± 9.0 
                 0.0089 
                  187 ± 7.4 
                 0.988 
                 104 ± 21 
                 0.382 
                  88 ± 9.0 
                 0.204 
                 51.6 ± 5.2  
                 0.033 
               
               
                 CXCL1 
                 24.5 ± 0.5 
                 &lt;0.0001 
                 28.2 ± 1.9 
                 0.577 
                 19.8 ± 1.8 
                 0.36 
                 12.8 ± 2.5  
                 0.005 
                 5.2 ± 1.0 
                 &lt;0.0001 
               
               
                 TNFSF15 
                  9.6 ± 1.1 
                 0.0012 
                     10 ± 1.1 
                 0.998 
                  7.6 ± 0.9 
                 0.544 
                 5.4 ± 0.8 
                 0.053 
                 2.9 ± 0.6 
                 0.003 
               
               
                 CCL5 
                  7.6 ± 0.9 
                 0.0045 
                  3.6 ± 0.8 
                 0.26 
                   3 ± 0.5 
                 0.018 
                 2.7 ± 0.2 
                 0.008 
                 2.6 ± 1.2 
                 0.006 
               
               
                 CCL4 
                 188 ± 12 
                 0.9519 
                 188 ± 16 
                 ns 
                 174 ± 41 
                 ns 
                 191 ± 57  
                 ns 
                 217 ± 51  
                 ns 
               
               
                 CCL20 
                  82.5 ± 27.8 
                 0.1189 
                     106 ± 15.1 
                 ns 
                     63 ± 3.1 
                 ns 
                 63.4 ± 1.0  
                 ns 
                 42.7 ± 12.7 
                 ns 
               
               
                 CXCL2 
                     122 ± 11.0 
                 0.9887 
                  125 ± 4.6 
                 ns 
                     128 ± 20.0 
                 ns 
                  132 ± 22.9 
                 ns 
                 130 ± 6.4  
                 ns 
               
               
                 TNF 
                     115 ± 13/1 
                 0.6112 
                 66.4 ± 9.6 
                 ns 
                   87 ± 12.4 
                 ns 
                 95.9 ± 21.2 
                 ns 
                     80 ± 14.5 
                 ns 
               
               
                 BMP6 
                  8.1 ± 1.8 
                 0.1195 
                  4.1 ± 1.1 
                 ns 
                  8.9 ± 1.7 
                 ns 
                 7.8 ± 1.1 
                 ns 
                 3.9 ± 0.5 
                 ns 
               
               
                   
               
               
                   1 All values are fold change mRNA levels vs. unstimulated PMA-differentiated THP1 cells 
               
               
                   2 Cells were preincubated with 0.4% DMSO or inhibitors for 1 h, then stimulated with 100 ng/ml LPS for 2 h. 
               
               
                   3 P-values from one-way ANOVA. 
               
               
                   4 P-values from Tukey Honestly Significant Difference post hoc test. 
               
            
           
         
       
     
     Example 5: Comparing Effects of SB203580 and UM101 on TNFα-Induced Gene Expression in HMVECLs 
     The effects of UM101 and SB203580 on TNFα-induced gene expression in HMVECLs using RNASeq were compared. HMVECLs were pretreated for 1 h with 10 μM SB203580 or 100 μM UM101, and then stimulated with 10 ng/ml TNFα for 3 h. A UM101 concentration 10-fold higher than its biologically effective dose in HMVECL barrier assays was used, to ensure identifying any partial overlap with SB203580. The TNFα concentration and duration of stimulation used were based on previously published studies and confirmed by preliminary qRT-PCR analysis of IL-8 and IL-1b mRNA expression ( FIG.  4   ). After filtering the RNASeq results for genes having 210 reads in at least one sample per experiment, 511 genes that were upregulated and 520 downregulated by 2-fold by TNFα treatment were found (Table 5). 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 RNASeq results filtered for genes with at least 10 reads in one sample per set 
               
            
           
           
               
               
            
               
                   
                 Log (base 2) fold-change 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 UM101 vs. 
                 SB203580 vs. 
                   
                   
                   
               
               
                 Gene 
                 control 
                 control 
                 bin 
                 Gene Symbol 
                 Gene Name 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 ENSG00000006468 
                 1.14697638 
                 1.42107626 
                 1 
                 ETV1 
                 ets variant 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 3490] 
               
               
                 ENSG00000128917 
                 1.25167179 
                 1.33201352 
                 1 
                 DLL4 
                 delta-like 4 ( Drosophila ) 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 2910] 
               
               
                 ENSG00000196872 
                 1.10366409 
                 1.62514778 
                 1 
                 KIAA1211L 
                 KIAA1211-like 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33454] 
               
               
                 ENSG00000108984 
                 1.34524718 
                 1.27752638 
                 1 
                 MAP2K6 
                 mitogen-activated protein 
               
               
                   
                   
                   
                   
                   
                 kinase kinase 6 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6846] 
               
               
                 ENSG00000229953 
                 1.63323083 
                 1.70115175 
                 1 
                 RP11-284F21.7 
               
               
                 ENSG00000255690 
                 1.81451563 
                 1.5932836 
                 1 
                 TRIL 
                 TLR4 interactor with 
               
               
                   
                   
                   
                   
                   
                 leucine-rich repeats 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 22200] 
               
               
                 ENSG00000095739 
                 1.11321658 
                 1.12281058 
                 1 
                 BAMBI 
                 BMP and activin membrane- 
               
               
                   
                   
                   
                   
                   
                 bound inhibitor 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30251] 
               
               
                 ENSG00000137872 
                 2.48510888 
                 1.90449301 
                 1 
                 SEMA6D 
                 sema domain, 
               
               
                   
                   
                   
                   
                   
                 transmembrane domain 
               
               
                   
                   
                   
                   
                   
                 (TM), and cytoplasmic 
               
               
                   
                   
                   
                   
                   
                 domain, (semaphorin) 6D 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 16770] 
               
               
                 ENSG00000184185 
                 1.18112212 
                 1.55055314 
                 1 
                 KCNJ12 
                 potassium channel, inwardly 
               
               
                   
                   
                   
                   
                   
                 rectifying subfamily J, 
               
               
                   
                   
                   
                   
                   
                 member 12 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6258] 
               
               
                 ENSG00000125848 
                 1.25784422 
                 1.46432561 
                 1 
                 FLRT3 
                 fibronectin leucine rich 
               
               
                   
                   
                   
                   
                   
                 transmembrane protein 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 3762] 
               
               
                 ENSG00000196664 
                 −1.5535936 
                 1.21794414 
                 2 
                 TLR7 
                 toll-like receptor 7 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 15631] 
               
               
                 ENSG00000119714 
                 −1.3040616 
                 1.22615088 
                 2 
                 GPR68 
                 G protein-coupled receptor 
               
               
                   
                   
                   
                   
                   
                 68 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4519] 
               
               
                 ENSG00000165379 
                 −1.4439353 
                 2.06145291 
                 2 
                 LRFN5 
                 leucine rich repeat and 
               
               
                   
                   
                   
                   
                   
                 fibronectin type III domain 
               
               
                   
                   
                   
                   
                   
                 containing 5 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20360] 
               
               
                 ENSG00000135378 
                 −1.1984882 
                 −1.78258053 
                 3 
                 PRRG4 
                 proline rich Gla (G- 
               
               
                   
                   
                   
                   
                   
                 carboxyglutamic acid) 4 
               
               
                   
                   
                   
                   
                   
                 (transmembrane) 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30799] 
               
               
                 ENSG00000145777 
                 −1.3366525 
                 −1.65143959 
                 3 
                 TSLP 
                 thymic stromal 
               
               
                   
                   
                   
                   
                   
                 lymphopoietin 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30743] 
               
               
                 ENSG00000102970 
                 −2.7303098 
                 −1.83414345 
                 3 
                 CCL17 
                 chemokine (C-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 17 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10615] 
               
               
                 ENSG00000259717 
                 −1.4952254 
                 −1.6963101 
                 3 
                 LINC00677 
                 long intergenic non-protein 
               
               
                   
                   
                   
                   
                   
                 coding RNA 677 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20121] 
               
               
                 ENSG00000205436 
                 −1.160471 
                 −1.47916318 
                 3 
                 EXOC3L4 
                 exocyst complex component 
               
               
                   
                   
                   
                   
                   
                 3-like 4 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20120] 
               
               
                 ENSG00000100985 
                 −1.0911796 
                 −1.15709135 
                 3 
                 MMP9 
                 matrix metallopeptidase 9 
               
               
                   
                   
                   
                   
                   
                 (gelatinase B, 92 kDa 
               
               
                   
                   
                   
                   
                   
                 gelatinase, 92 kDa type IV 
               
               
                   
                   
                   
                   
                   
                 collagenase) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7176] 
               
               
                 ENSG00000131203 
                 −3.5679874 
                 −3.51063293 
                 3 
                 IDO1 
                 indoleamine 2,3-dioxygenase 
               
               
                   
                   
                   
                   
                   
                 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6059] 
               
               
                 ENSG00000276408 
                 −3.0213604 
                 −2.36477309 
                 3 
                 RP11-490B18.5 
               
               
                 ENSG00000169245 
                 −4.3698367 
                 −3.10091556 
                 3 
                 CXCL10 
                 chemokine (C-X-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 10 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10637] 
               
               
                 ENSG00000091972 
                 −1.5381554 
                 −1.72928565 
                 3 
                 CD200 
                 CD200 molecule 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7203] 
               
               
                 ENSG00000110446 
                 −1.7310589 
                 −1.00338842 
                 3 
                 SLC15A3 
                 solute carrier family 15 
               
               
                   
                   
                   
                   
                   
                 (oligopeptide transporter), 
               
               
                   
                   
                   
                   
                   
                 member 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18068] 
               
               
                 ENSG00000111424 
                 −1.1973169 
                 −1.16718631 
                 3 
                 VDR 
                 vitamin D (1,25- 
               
               
                   
                   
                   
                   
                   
                 dihydroxyvitamin D3) 
               
               
                   
                   
                   
                   
                   
                 receptor [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 12679] 
               
               
                 ENSG00000125538 
                 −1.1725305 
                 −1.40158693 
                 3 
                 IL1B 
                 interleukin 1, beta 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5992] 
               
               
                 ENSG00000279805 
                 −1.4538855 
                 −1.28507313 
                 3 
                 CTA-212A2.1 
               
               
                 ENSG00000202533 
                 −2.8157077 
                 −1.96733528 
                 3 
                 Y_RNA 
                 Y RNA 
               
               
                   
                   
                   
                   
                   
                 [Source: RFAM; Acc: 
               
               
                   
                   
                   
                   
                   
                 RF00019] 
               
               
                 ENSG00000181656 
                 −2.1505992 
                 −1.39708375 
                 3 
                 GPR88 
                 G protein-coupled receptor 
               
               
                   
                   
                   
                   
                   
                 88 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4539] 
               
               
                 ENSG00000116031 
                 −3.3824373 
                 −1.54775729 
                 3 
                 CD207 
                 CD207 molecule, langerin 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17935] 
               
               
                 ENSG00000159450 
                 −1.5476653 
                 −1.50495809 
                 3 
                 TCHH 
                 trichohyalin [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11791] 
               
               
                 ENSG00000103044 
                 −1.1243396 
                 −1.43377734 
                 3 
                 HAS3 
                 hyaluronan synthase 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4820] 
               
               
                 ENSG00000225492 
                 −1.7557061 
                 −1.3632872 
                 3 
                 GBP1P1 
                 guanylate binding protein 1, 
               
               
                   
                   
                   
                   
                   
                 interferon-inducible 
               
               
                   
                   
                   
                   
                   
                 pseudogene 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 39561] 
               
               
                 ENSG00000145113 
                 −1.0573157 
                 −2.85949188 
                 3 
                 MUC4 
                 mucin 4, cell surface 
               
               
                   
                   
                   
                   
                   
                 associated [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7514] 
               
               
                 ENSG00000164181 
                 −1.3810632 
                 −1.34093337 
                 3 
                 ELOVL7 
                 ELOVL fatty acid elongase 7 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 26292] 
               
               
                 ENSG00000169248 
                 −4.1363549 
                 −1.37790594 
                 3 
                 CXCL11 
                 chemokine (C-X-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 11 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10638] 
               
               
                 ENSG00000162654 
                 −2.8359479 
                 −1.25928308 
                 3 
                 GBP4 
                 guanylate binding protein 4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20480] 
               
               
                 ENSG00000144837 
                 −1.5006334 
                 −1.27452433 
                 3 
                 PLA1A 
                 phospholipase A1 member A 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17661] 
               
               
                 ENSG00000222365 
                 −1.7462552 
                 −2.69898993 
                 3 
                 SNORD12B 
                 small nucleolar RNA, C/D 
               
               
                   
                   
                   
                   
                   
                 box 12B [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33573] 
               
               
                 ENSG00000237988 
                 −3.7376706 
                 −1.08751395 
                 3 
                 OR2I1P 
                 olfactory receptor, family 2, 
               
               
                   
                   
                   
                   
                   
                 subfamily I, member 1 
               
               
                   
                   
                   
                   
                   
                 pseudogene [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8258] 
               
               
                 ENSG00000163735 
                 −1.4683077 
                 −1.01742785 
                 3 
                 CXCL5 
                 chemokine (C-X-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 5 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10642] 
               
               
                 ENSG00000277105 
                 1.8720451 
                 −1.66179692 
                 4 
                 FP236383.10 
               
               
                 ENSG00000259498 
                 1.51730088 
                 0 
                 5 
                 RP11-244F12.3 
               
               
                 ENSG00000079841 
                 1.1627696 
                 0 
                 5 
                 RIMS1 
                 regulating synaptic 
               
               
                   
                   
                   
                   
                   
                 membrane exocytosis 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17282] 
               
               
                 ENSG00000104081 
                 1.87839947 
                 0 
                 5 
                 BMF 
                 Bcl2 modifying factor 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 24132] 
               
               
                 ENSG00000128011 
                 1.21234677 
                 0 
                 5 
                 LRFN1 
                 leucine rich repeat and 
               
               
                   
                   
                   
                   
                   
                 fibronectin type III domain 
               
               
                   
                   
                   
                   
                   
                 containing 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 29290] 
               
               
                 ENSG00000102760 
                 2.39873086 
                 0 
                 5 
                 RGCC 
                 regulator of cell cycle 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20369] 
               
               
                 ENSG00000272918 
                 2.02570082 
                 0 
                 5 
                 CTB-152G17.6 
               
               
                 ENSG00000158715 
                 1.09721113 
                 0 
                 5 
                 SLC45A3 
                 solute carrier family 45, 
               
               
                   
                   
                   
                   
                   
                 member 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8642] 
               
               
                 ENSG00000169247 
                 1.22087519 
                 0 
                 5 
                 SH3TC2 
                 SH3 domain and 
               
               
                   
                   
                   
                   
                   
                 tetratricopeptide repeats 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 29427] 
               
               
                 ENSG00000163235 
                 1.00798575 
                 0 
                 5 
                 TGFA 
                 transforming growth factor, 
               
               
                   
                   
                   
                   
                   
                 alpha [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11765] 
               
               
                 ENSG00000138311 
                 1.68539518 
                 0 
                 5 
                 ZNF365 
                 zinc finger protein 365 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18194] 
               
               
                 ENSG00000263426 
                 1.90751532 
                 0 
                 5 
                 RN7SL471P 
                 RNA, 7SL, cytoplasmic 471, 
               
               
                   
                   
                   
                   
                   
                 pseudogene [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 46487] 
               
               
                 ENSG00000203883 
                 3.1401464 
                 0 
                 5 
                 SOX18 
                 SRY (sex determining region 
               
               
                   
                   
                   
                   
                   
                 Y)-box 18 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11194] 
               
               
                 ENSG00000152213 
                 2.11880858 
                 0 
                 5 
                 ARL11 
                 ADP-ribosylation factor-like 
               
               
                   
                   
                   
                   
                   
                 11 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 24046] 
               
               
                 ENSG00000115641 
                 1.20543525 
                 0 
                 5 
                 FHL2 
                 four and a half LIM domains 
               
               
                   
                   
                   
                   
                   
                 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 3703] 
               
               
                 ENSG00000163884 
                 1.31377126 
                 0 
                 5 
                 KLF15 
                 Kruppel-like factor 15 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14536] 
               
               
                 ENSG00000171223 
                 1.07792014 
                 0 
                 5 
                 JUNB 
                 jun B proto-oncogene 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6205] 
               
               
                 ENSG00000137875 
                 1.24981 
                 0 
                 5 
                 BCL2L10 
                 BCL2-like 10 (apoptosis 
               
               
                   
                   
                   
                   
                   
                 facilitator) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 993] 
               
               
                 ENSG00000119630 
                 1.02473901 
                 0 
                 5 
                 PGF 
                 placental growth factor 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8893] 
               
               
                 ENSG00000157404 
                 1.98981566 
                 0 
                 5 
                 KIT 
                 v-kit Hardy-Zuckerman 4 
               
               
                   
                   
                   
                   
                   
                 feline sarcoma viral 
               
               
                   
                   
                   
                   
                   
                 oncogene homolog 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6342] 
               
               
                 ENSG00000004799 
                 2.74875752 
                 0 
                 5 
                 PDK4 
                 pyruvate dehydrogenase 
               
               
                   
                   
                   
                   
                   
                 kinase, isozyme 4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8812] 
               
               
                 ENSG00000104903 
                 1.17436711 
                 0 
                 5 
                 LYL1 
                 lymphoblastic leukemia 
               
               
                   
                   
                   
                   
                   
                 associated hematopoiesis 
               
               
                   
                   
                   
                   
                   
                 regulator 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6734] 
               
               
                 ENSG00000164683 
                 2.56462289 
                 0 
                 5 
                 HEY1 
                 hes-related family bHLH 
               
               
                   
                   
                   
                   
                   
                 transcription factor with 
               
               
                   
                   
                   
                   
                   
                 YRPW motif 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4880] 
               
               
                 ENSG00000229436 
                 2.97587733 
                 0 
                 5 
                 AC073850.6 
               
               
                 ENSG00000074590 
                 1.86606392 
                 0 
                 5 
                 NUAK1 
                 NUAK family, SNF1-like 
               
               
                   
                   
                   
                   
                   
                 kinase, 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14311] 
               
               
                 ENSG00000163121 
                 1.17777496 
                 0 
                 5 
                 NEURL3 
                 neuralized E3 ubiquitin 
               
               
                   
                   
                   
                   
                   
                 protein ligase 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 25162] 
               
               
                 ENSG00000171435 
                 2.0275154 
                 0 
                 5 
                 KSR2 
                 kinase suppressor of ras 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18610] 
               
               
                 ENSG00000225213 
                 2.78814593 
                 0 
                 5 
                 RP11-197M22.2 
               
               
                 ENSG00000175556 
                 2.1400816 
                 0 
                 5 
                 LONRF3 
                 LON peptidase N-terminal 
               
               
                   
                   
                   
                   
                   
                 domain and ring finger 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 21152] 
               
               
                 ENSG00000172031 
                 1.46275504 
                 0 
                 5 
                 EPHX4 
                 epoxide hydrolase 4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23758] 
               
               
                 ENSG00000164284 
                 1.60799521 
                 0 
                 5 
                 GRPEL2 
                 GrpE-like 2, mitochondrial 
               
               
                   
                   
                   
                   
                   
                 ( E. coli ) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 21060] 
               
               
                 ENSG00000198774 
                 1.92290695 
                 0 
                 5 
                 RASSF9 
                 Ras association 
               
               
                   
                   
                   
                   
                   
                 (RalGDS/AF-6) domain 
               
               
                   
                   
                   
                   
                   
                 family (N-terminal) member 
               
               
                   
                   
                   
                   
                   
                 9 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 15739] 
               
               
                 ENSG00000109452 
                 1.25820227 
                 0 
                 5 
                 INPP4B 
                 inositol polyphosphate-4- 
               
               
                   
                   
                   
                   
                   
                 phosphatase, type II, 105 kDa 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6075] 
               
               
                 ENSG00000071282 
                 1.2458539 
                 0 
                 5 
                 LMCD1 
                 LIM and cysteine-rich 
               
               
                   
                   
                   
                   
                   
                 domains 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6633] 
               
               
                 ENSG00000163545 
                 1.15652631 
                 0 
                 5 
                 NUAK2 
                 NUAK family, SNF1-like 
               
               
                   
                   
                   
                   
                   
                 kinase, 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 29558] 
               
               
                 ENSG00000125968 
                 2.63377664 
                 0 
                 5 
                 ID1 
                 inhibitor of DNA binding 1, 
               
               
                   
                   
                   
                   
                   
                 dominant negative helix- 
               
               
                   
                   
                   
                   
                   
                 loop-helix protein 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5360] 
               
               
                 ENSG00000099260 
                 1.50498454 
                 0 
                 5 
                 PALMD 
                 palmdelphin [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 15846] 
               
               
                 ENSG00000176641 
                 1.2877484 
                 0 
                 5 
                 RNF152 
                 ring finger protein 152 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 26811] 
               
               
                 ENSG00000139874 
                 1.37617951 
                 0 
                 5 
                 SSTR1 
                 somatostatin receptor 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11330] 
               
               
                 ENSG00000137834 
                 2.51438228 
                 0 
                 5 
                 SMAD6 
                 SMAD family member 6 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6772] 
               
               
                 ENSG00000259721 
                 1.0709029 
                 0 
                 5 
                 RP11-758N13.1 
               
               
                 ENSG00000181800 
                 2.42803687 
                 0 
                 5 
                 CELF2-AS1 
                 CELF2 antisense RNA 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23515] 
               
               
                 ENSG00000184523 
                 2.14599043 
                 0 
                 5 
                 PTGER4P2 
                 prostaglandin E receptor 4 
               
               
                   
                   
                   
                   
                   
                 (subtype EP4) pseudogene 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 9598] 
               
               
                 ENSG00000101187 
                 1.12788457 
                 0 
                 5 
                 SLCO4A1 
                 solute carrier organic anion 
               
               
                   
                   
                   
                   
                   
                 transporter family, member 
               
               
                   
                   
                   
                   
                   
                 4A1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10953] 
               
               
                 ENSG00000237512 
                 1.72226772 
                 0 
                 5 
                 UNC5B-AS1 
                 UNC5B antisense RNA 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 45096] 
               
               
                 ENSG00000156463 
                 1.43711148 
                 0 
                 5 
                 SH3RF2 
                 SH3 domain containing ring 
               
               
                   
                   
                   
                   
                   
                 finger 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 26299] 
               
               
                 ENSG00000137672 
                 1.41739616 
                 0 
                 5 
                 TRPC6 
                 transient receptor potential 
               
               
                   
                   
                   
                   
                   
                 cation channel, subfamily C, 
               
               
                   
                   
                   
                   
                   
                 member 6 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 12338] 
               
               
                 ENSG00000138135 
                 2.4356406 
                 0 
                 5 
                 CH25H 
                 cholesterol 25-hydroxylase 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1907] 
               
               
                 ENSG00000183691 
                 1.18113108 
                 0 
                 5 
                 NOG 
                 noggin [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7866] 
               
               
                 ENSG00000139174 
                 2.23155754 
                 0 
                 5 
                 PRICKLE1 
                 prickle homolog 1 
               
               
                   
                   
                   
                   
                   
                 ( Drosophila ) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17019] 
               
               
                 ENSG00000188305 
                 1.46592995 
                 0 
                 5 
                 C19orf35 
                 chromosome 19 open reading 
               
               
                   
                   
                   
                   
                   
                 frame 35 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 24793] 
               
               
                 ENSG00000082497 
                 3.01359039 
                 0 
                 5 
                 SERTAD4 
                 SERTA domain containing 4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 25236] 
               
               
                 ENSG00000134215 
                 1.69084 
                 0 
                 5 
                 VAV3 
                 vav 3 guanine nucleotide 
               
               
                   
                   
                   
                   
                   
                 exchange factor 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 12659] 
               
               
                 ENSG00000242902 
                 1.87508823 
                 0 
                 5 
                 RP11-309L24.2 
               
               
                 ENSG00000027075 
                 1.03323842 
                 0 
                 5 
                 PRKCH 
                 protein kinase C, eta 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 9403] 
               
               
                 ENSG00000203280 
                 1.22563855 
                 0 
                 5 
                 CTA-221G9.12 
               
               
                 ENSG00000006459 
                 1.00029861 
                 0 
                 5 
                 KDM7A 
                 lysine (K)-specific 
               
               
                   
                   
                   
                   
                   
                 demethylase 7A 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 22224] 
               
               
                 ENSG00000171408 
                 3.15161753 
                 0 
                 5 
                 PDE7B 
                 phosphodiesterase 7B 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8792] 
               
               
                 ENSG00000162981 
                 1.53241836 
                 0 
                 5 
                 FAM84A 
                 family with sequence 
               
               
                   
                   
                   
                   
                   
                 similarity 84, member A 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20743] 
               
               
                 ENSG00000118946 
                 1.93243268 
                 0 
                 5 
                 PCDH17 
                 protocadherin 17 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14267] 
               
               
                 ENSG00000146376 
                 1.27126839 
                 0 
                 5 
                 ARHGAP18 
                 Rho GTPase activating 
               
               
                   
                   
                   
                   
                   
                 protein 18 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 21035] 
               
               
                 ENSG00000204086 
                 2.05144911 
                 0 
                 5 
                 RPA4 
                 replication protein A4, 
               
               
                   
                   
                   
                   
                   
                 30 kDa [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30305] 
               
               
                 ENSG00000221887 
                 1.05893107 
                 0 
                 5 
                 HMSD 
                 histocompatibility (minor) 
               
               
                   
                   
                   
                   
                   
                 serpin domain containing 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23037] 
               
               
                 ENSG00000196196 
                 1.26354255 
                 0 
                 5 
                 HRCT1 
                 histidine rich carboxyl 
               
               
                   
                   
                   
                   
                   
                 terminus 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33872] 
               
               
                 ENSG00000172548 
                 2.84971177 
                 0 
                 5 
                 NIPAL4 
                 NIPA-like domain 
               
               
                   
                   
                   
                   
                   
                 containing 4 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 28018] 
               
               
                 ENSG00000156804 
                 2.04515428 
                 0 
                 5 
                 FBXO32 
                 F-box protein 32 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 16731] 
               
               
                 ENSG00000203684 
                 1.5984677 
                 0 
                 5 
                 IBA57-AS1 
                 IBA57 antisense RNA 1 
               
               
                   
                   
                   
                   
                   
                 (head to head) 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 32062] 
               
               
                 ENSG00000205502 
                 1.63750711 
                 0 
                 5 
                 C2CD4B 
                 C2 calcium-dependent 
               
               
                   
                   
                   
                   
                   
                 domain containing 4B 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33628] 
               
               
                 ENSG00000163734 
                 1.26141193 
                 0 
                 5 
                 CXCL3 
                 chemokine (C-X-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4604] 
               
               
                 ENSG00000181444 
                 1.48713949 
                 0 
                 5 
                 ZNF467 
                 zinc finger protein 467 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23154] 
               
               
                 ENSG00000275342 
                 1.45192287 
                 0 
                 5 
                 SGK223 
                 Tyrosine-protein kinase 
               
               
                   
                   
                   
                   
                   
                 SgK223 
               
               
                   
                   
                   
                   
                   
                 [Source: UniProtKB/Swiss- 
               
               
                   
                   
                   
                   
                   
                 Prot; Acc: Q86YV5] 
               
               
                 ENSG00000214944 
                 1.43116765 
                 0 
                 5 
                 ARHGEF28 
                 Rho guanine nucleotide 
               
               
                   
                   
                   
                   
                   
                 exchange factor (GEF) 28 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30322] 
               
               
                 ENSG00000198795 
                 1.37664308 
                 0 
                 5 
                 ZNF521 
                 zinc finger protein 521 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 24605] 
               
               
                 ENSG00000108932 
                 1.95156031 
                 0 
                 5 
                 SLC16A6 
                 solute carrier family 16, 
               
               
                   
                   
                   
                   
                   
                 member 6 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10927] 
               
               
                 ENSG00000145990 
                 1.18764084 
                 0 
                 5 
                 GFOD1 
                 glucose-fructose 
               
               
                   
                   
                   
                   
                   
                 oxidoreductase domain 
               
               
                   
                   
                   
                   
                   
                 containing 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 21096] 
               
               
                 ENSG00000179546 
                 1.7287987 
                 0 
                 5 
                 HTR1D 
                 5-hydroxytryptamine 
               
               
                   
                   
                   
                   
                   
                 (serotonin) receptor 1D, G 
               
               
                   
                   
                   
                   
                   
                 protein-coupled 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5289] 
               
               
                 ENSG00000186472 
                 1.73331066 
                 0 
                 5 
                 PCLO 
                 piccolo presynaptic 
               
               
                   
                   
                   
                   
                   
                 cytomatrix protein 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 13406] 
               
               
                 ENSG00000138678 
                 1.55650245 
                 0 
                 5 
                 AGPAT9 
                 1-acylglycerol-3-phosphate 
               
               
                   
                   
                   
                   
                   
                 O-acyltransferase 9 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 28157] 
               
               
                 ENSG00000225814 
                 1.57236046 
                 0 
                 5 
                 GRPEL2P2 
                 GrpE-like 2, mitochondrial 
               
               
                   
                   
                   
                   
                   
                 ( E. coli ) pseudogene 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 41970] 
               
               
                 ENSG00000172572 
                 1.01708765 
                 0 
                 5 
                 PDE3A 
                 phosphodiesterase 3A, 
               
               
                   
                   
                   
                   
                   
                 cGMP-inhibited 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8778] 
               
               
                 ENSG00000107282 
                 1.0986938 
                 0 
                 5 
                 APBA1 
                 amyloid beta (A4) precursor 
               
               
                   
                   
                   
                   
                   
                 protein-binding, family A, 
               
               
                   
                   
                   
                   
                   
                 member 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 578] 
               
               
                 ENSG00000171877 
                 1.0590391 
                 0 
                 5 
                 FRMD5 
                 FERM domain containing 5 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 28214] 
               
               
                 ENSG00000151623 
                 1.83557493 
                 0 
                 5 
                 NR3C2 
                 nuclear receptor subfamily 3, 
               
               
                   
                   
                   
                   
                   
                 group C, member 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7979] 
               
               
                 ENSG00000189184 
                 1.39874706 
                 0 
                 5 
                 PCDH18 
                 protocadherin 18 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14268] 
               
               
                 ENSG00000187479 
                 1.56424224 
                 0 
                 5 
                 C11orf96 
                 chromosome 11 open reading 
               
               
                   
                   
                   
                   
                   
                 frame 96 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 38675] 
               
               
                 ENSG00000178726 
                 1.31386114 
                 0 
                 5 
                 THBD 
                 thrombomodulin 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11784] 
               
               
                 ENSG00000137193 
                 2.05607477 
                 0 
                 5 
                 PIM1 
                 Pim-1 proto-oncogene, 
               
               
                   
                   
                   
                   
                   
                 serine/threonine kinase 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8986] 
               
               
                 ENSG00000154734 
                 1.08386589 
                 0 
                 5 
                 ADAMTS1 
                 ADAM metallopeptidase 
               
               
                   
                   
                   
                   
                   
                 with thrombospondin type 1 
               
               
                   
                   
                   
                   
                   
                 motif, 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 217] 
               
               
                 ENSG00000143772 
                 1.05692317 
                 0 
                 5 
                 ITPKB 
                 inositol-trisphosphate 3- 
               
               
                   
                   
                   
                   
                   
                 kinase B [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6179] 
               
               
                 ENSG00000140022 
                 1.42852098 
                 0 
                 5 
                 STON2 
                 stonin 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30652] 
               
               
                 ENSG00000181722 
                 1.75771309 
                 0 
                 5 
                 ZBTB20 
                 zinc finger and BTB domain 
               
               
                   
                   
                   
                   
                   
                 containing 20 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 13503] 
               
               
                 ENSG00000184058 
                 2.32518737 
                 0 
                 5 
                 TBX1 
                 T-box 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11592] 
               
               
                 ENSG00000043591 
                 1.38727807 
                 0 
                 5 
                 ADRB1 
                 adrenoceptor beta 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 285] 
               
               
                 ENSG00000126550 
                 2.94676056 
                 0 
                 5 
                 HTN1 
                 histatin 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5283] 
               
               
                 ENSG00000143867 
                 1.2361215 
                 0 
                 5 
                 OSR1 
                 odd-skipped related 
               
               
                   
                   
                   
                   
                   
                 transciption factor 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8111] 
               
               
                 ENSG00000116833 
                 1.34604824 
                 0 
                 5 
                 NR5A2 
                 nuclear receptor subfamily 5, 
               
               
                   
                   
                   
                   
                   
                 group A, member 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7984] 
               
               
                 ENSG00000166292 
                 2.00173044 
                 0 
                 5 
                 TMEM100 
                 transmembrane protein 100 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 25607] 
               
               
                 ENSG00000188487 
                 1.04413181 
                 0 
                 5 
                 INSC 
                 inscuteable homolog 
               
               
                   
                   
                   
                   
                   
                 ( Drosophila ) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33116] 
               
               
                 ENSG00000176697 
                 1.76616859 
                 0 
                 5 
                 BDNF 
                 brain-derived neurotrophic 
               
               
                   
                   
                   
                   
                   
                 factor [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1033] 
               
               
                 ENSG00000079102 
                 1.5770273 
                 0 
                 5 
                 RUNX1T1 
                 runt-related transcription 
               
               
                   
                   
                   
                   
                   
                 factor 1; translocated to, 1 
               
               
                   
                   
                   
                   
                   
                 (cyclin D-related) 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1535] 
               
               
                 ENSG00000162599 
                 1.02633907 
                 0 
                 5 
                 NFIA 
                 nuclear factor I/A 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7784] 
               
               
                 ENSG00000188763 
                 1.52543754 
                 0 
                 5 
                 FZD9 
                 frizzled class receptor 9 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4047] 
               
               
                 ENSG00000154639 
                 1.36734426 
                 0 
                 5 
                 CXADR 
                 coxsackie virus and 
               
               
                   
                   
                   
                   
                   
                 adenovirus receptor 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 2559] 
               
               
                 ENSG00000227946 
                 1.24976159 
                 0 
                 5 
                 AC007383.3 
               
               
                 ENSG00000143341 
                 1.16130281 
                 0 
                 5 
                 HMCN1 
                 hemicentin 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 19194] 
               
               
                 ENSG00000237892 
                 1.07996952 
                 0 
                 5 
                 KLF7-IT1 
                 KLF7 intronic transcript 1 
               
               
                   
                   
                   
                   
                   
                 (non-protein coding) 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 41355] 
               
               
                 ENSG00000103522 
                 1.30364536 
                 0 
                 5 
                 IL21R 
                 interleukin 21 receptor 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6006] 
               
               
                 ENSG00000162630 
                 1.457488 
                 0 
                 5 
                 B3GALT2 
                 UDP-Gal: betaGlcNAc beta 
               
               
                   
                   
                   
                   
                   
                 1,3-galactosyltransferase, 
               
               
                   
                   
                   
                   
                   
                 polypeptide 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 917] 
               
               
                 ENSG00000106069 
                 1.15244395 
                 0 
                 5 
                 CHN2 
                 chimerin 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1944] 
               
               
                 ENSG00000169047 
                 1.03338349 
                 0 
                 5 
                 IRS1 
                 insulin receptor substrate 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6125] 
               
               
                 ENSG00000226476 
                 1.25685284 
                 0 
                 5 
                 RP11-776H12.1 
               
               
                 ENSG00000181016 
                 1.30372056 
                 0 
                 5 
                 LSMEM1 
                 leucine-rich single-pass 
               
               
                   
                   
                   
                   
                   
                 membrane protein 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 22036] 
               
               
                 ENSG00000121966 
                 3.89708969 
                 0 
                 5 
                 CXCR4 
                 chemokine (C-X-C motif) 
               
               
                   
                   
                   
                   
                   
                 receptor 4 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 2561] 
               
               
                 ENSG00000189143 
                 1.4778446 
                 0 
                 5 
                 CLDN4 
                 claudin 4 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 2046] 
               
               
                 ENSG00000257642 
                 2.54048396 
                 0 
                 5 
                 RP11-474B16.1 
               
               
                 ENSG00000250271 
                 2.3827306 
                 0 
                 5 
                 RP11-64D22.5 
               
               
                 ENSG00000188483 
                 1.76997593 
                 0 
                 5 
                 IER5L 
                 immediate early response 5- 
               
               
                   
                   
                   
                   
                   
                 like [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23679] 
               
               
                 ENSG00000183775 
                 1.04020782 
                 0 
                 5 
                 KCTD16 
                 potassium channel 
               
               
                   
                   
                   
                   
                   
                 tetramerization domain 
               
               
                   
                   
                   
                   
                   
                 containing 16 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 29244] 
               
               
                 ENSG00000107984 
                 1.55122779 
                 0 
                 5 
                 DKK1 
                 dickkopf WNT signaling 
               
               
                   
                   
                   
                   
                   
                 pathway inhibitor 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 2891] 
               
               
                 ENSG00000174514 
                 0 
                 1.18746783 
                 6 
                 MFSD4 
                 major facilitator superfamily 
               
               
                   
                   
                   
                   
                   
                 domain containing 4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 25433] 
               
               
                 ENSG00000270379 
                 0 
                 1.09045988 
                 6 
                 HEATR9 
                 HEAT repeat containing 9 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 26548] 
               
               
                 ENSG00000240859 
                 0 
                 1.36188025 
                 6 
                 AC093627.10 
               
               
                 ENSG00000236671 
                 0 
                 1.65509644 
                 6 
                 PRKG1-AS1 
                 PRKG1 antisense RNA 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 45029] 
               
               
                 ENSG00000261707 
                 0 
                 1.15736629 
                 6 
                 RP11-264M12.2 
               
               
                 ENSG00000273669 
                 0 
                 3.69876021 
                 6 
                 RP11-405M12.4 
               
               
                 ENSG00000231345 
                 0 
                 1.35868618 
                 6 
                 BEND3P1 
                 BEN domain containing 3 
               
               
                   
                   
                   
                   
                   
                 pseudogene 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 45014] 
               
               
                 ENSG00000134253 
                 0 
                 1.10224143 
                 6 
                 TRIM45 
                 tripartite motif containing 45 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 19018] 
               
               
                 ENSG00000138336 
                 0 
                 1.76426632 
                 6 
                 TET1 
                 tet methylcytosine 
               
               
                   
                   
                   
                   
                   
                 dioxygenase 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 29484] 
               
               
                 ENSG00000120162 
                 0 
                 1.23378866 
                 6 
                 MOB3B 
                 MOB kinase activator 3B 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23825] 
               
               
                 ENSG00000171860 
                 0 
                 1.01814888 
                 6 
                 C3AR1 
                 complement component 3a 
               
               
                   
                   
                   
                   
                   
                 receptor 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1319] 
               
               
                 ENSG00000167676 
                 0 
                 1.03160807 
                 6 
                 PLIN4 
                 perilipin 4 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 29393] 
               
               
                 ENSG00000237234 
                 0 
                 1.66084244 
                 6 
                 RP1-142L7.5 
               
               
                 ENSG00000164124 
                 0 
                 1.05381028 
                 6 
                 TMEM144 
                 transmembrane protein 144 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 25633] 
               
               
                 ENSG00000118513 
                 0 
                 1.12982301 
                 6 
                 MYB 
                 v-myb avian myeloblastosis 
               
               
                   
                   
                   
                   
                   
                 viral oncogene homolog 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7545] 
               
               
                 ENSG00000091137 
                 0 
                 1.16699806 
                 6 
                 SLC26A4 
                 solute carrier family 26 
               
               
                   
                   
                   
                   
                   
                 (anion exchanger), member 4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8818] 
               
               
                 ENSG00000198483 
                 0 
                 1.43532228 
                 6 
                 ANKRD35 
                 ankyrin repeat domain 35 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 26323] 
               
               
                 ENSG00000237886 
                 0 
                 1.57281997 
                 6 
                 LINC01573 
                 long intergenic non-protein 
               
               
                   
                   
                   
                   
                   
                 coding RNA 1573 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 51192] 
               
               
                 ENSG00000174004 
                 0 
                 2.27602232 
                 6 
                 NRROS 
                 negative regulator of reactive 
               
               
                   
                   
                   
                   
                   
                 oxygen species 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 24613] 
               
               
                 ENSG00000185634 
                 0 
                 1.66433678 
                 6 
                 SHC4 
                 SHC (Src homology 2 
               
               
                   
                   
                   
                   
                   
                 domain containing) family, 
               
               
                   
                   
                   
                   
                   
                 member 4 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 16743] 
               
               
                 ENSG00000259886 
                 0 
                 1.17666464 
                 6 
               
               
                 ENSG00000145358 
                 0 
                 1.32550219 
                 6 
                 DDIT4L 
                 DNA-damage-inducible 
               
               
                   
                   
                   
                   
                   
                 transcript 4-like 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30555] 
               
               
                 ENSG00000269896 
                 0 
                 1.28838255 
                 6 
                 RP4-740C4.5 
               
               
                 ENSG00000135828 
                 0 
                 1.0837094 
                 6 
                 RNASEL 
                 ribonuclease L (2′,5′- 
               
               
                   
                   
                   
                   
                   
                 oligoisoadenylate synthetase- 
               
               
                   
                   
                   
                   
                   
                 dependent) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10050] 
               
               
                 ENSG00000259162 
                 0 
                 1.52667158 
                 6 
                 RP11-203M5.6 
               
               
                 ENSG00000279109 
                 −3.3403535 
                 0 
                 7 
                 AC008641.1 
                 Uncharacterized protein 
               
               
                   
                   
                   
                   
                   
                 {ECO: 0000313|Ensembl: 
               
               
                   
                   
                   
                   
                   
                 ENSP00000485568} 
               
               
                   
                   
                   
                   
                   
                 [Source: UniProtKB/TrEMBL; 
               
               
                   
                   
                   
                   
                   
                 Acc: A0A096LPF4] 
               
               
                 ENSG00000152778 
                 −1.0972226 
                 0 
                 7 
                 IFIT5 
                 interferon-induced protein 
               
               
                   
                   
                   
                   
                   
                 with tetratricopeptide repeats 
               
               
                   
                   
                   
                   
                   
                 5 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 13328] 
               
               
                 ENSG00000128284 
                 −1.3050716 
                 0 
                 7 
                 APOL3 
                 apolipoprotein L, 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14868] 
               
               
                 ENSG00000213886 
                 −4.0396114 
                 0 
                 7 
                 UBD 
                 ubiquitin D [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18795] 
               
               
                 ENSG00000164116 
                 −1.5223055 
                 0 
                 7 
                 GUCY1A3 
                 guanylate cyclase 1, soluble, 
               
               
                   
                   
                   
                   
                   
                 alpha 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4685] 
               
               
                 ENSG00000137462 
                 −1.0020489 
                 0 
                 7 
                 TLR2 
                 toll-like receptor 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11848] 
               
               
                 ENSG00000049249 
                 −2.1008906 
                 0 
                 7 
                 TNFRSF9 
                 tumor necrosis factor 
               
               
                   
                   
                   
                   
                   
                 receptor superfamily, 
               
               
                   
                   
                   
                   
                   
                 member 9 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11924] 
               
               
                 ENSG00000169181 
                 −2.095598 
                 0 
                 7 
                 GSG1L 
                 GSG1-like [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 28283] 
               
               
                 ENSG00000162888 
                 −1.7912286 
                 0 
                 7 
                 C1orf147 
                 chromosome 1 open reading 
               
               
                   
                   
                   
                   
                   
                 frame 147 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 32061] 
               
               
                 ENSG00000107201 
                 −1.2413536 
                 0 
                 7 
                 DDX58 
                 DEAD (Asp-Glu-Ala-Asp) 
               
               
                   
                   
                   
                   
                   
                 box polypeptide 58 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 19102] 
               
               
                 ENSG00000179826 
                 −2.5133806 
                 0 
                 7 
                 MRGPRX3 
                 MAS-related GPR, member 
               
               
                   
                   
                   
                   
                   
                 X3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17980] 
               
               
                 ENSG00000132109 
                 −1.2867007 
                 0 
                 7 
                 TRIM21 
                 tripartite motif containing 21 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11312] 
               
               
                 ENSG00000215007 
                 −1.0078729 
                 0 
                 7 
                 DNAJA1P3 
                 DnaJ (Hsp40) homolog, 
               
               
                   
                   
                   
                   
                   
                 subfamily A, member 1 
               
               
                   
                   
                   
                   
                   
                 pseudogene 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 39339] 
               
               
                 ENSG00000204682 
                 −1.1628447 
                 0 
                 7 
                 CASC10 
                 cancer susceptibility 
               
               
                   
                   
                   
                   
                   
                 candidate 10 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 31448] 
               
               
                 ENSG00000108688 
                 −1.8703428 
                 0 
                 7 
                 CCL7 
                 chemokine (C-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 7 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10634] 
               
               
                 ENSG00000112096 
                 −1.0730214 
                 0 
                 7 
                 SOD2 
                 superoxide dismutase 2, 
               
               
                   
                   
                   
                   
                   
                 mitochondrial 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11180] 
               
               
                 ENSG00000010379 
                 −2.697306 
                 0 
                 7 
                 SLC6A13 
                 solute carrier family 6 
               
               
                   
                   
                   
                   
                   
                 (neurotransmitter 
               
               
                   
                   
                   
                   
                   
                 transporter), member 13 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11046] 
               
               
                 ENSG00000169403 
                 −1.579388 
                 0 
                 7 
                 PTAFR 
                 platelet-activating factor 
               
               
                   
                   
                   
                   
                   
                 receptor [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 9582] 
               
               
                 ENSG00000115604 
                 −3.2590591 
                 0 
                 7 
                 IL18R1 
                 interleukin 18 receptor 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5988] 
               
               
                 ENSG00000133401 
                 −1.024655 
                 0 
                 7 
                 PDZD2 
                 PDZ domain containing 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18486] 
               
               
                 ENSG00000095587 
                 −2.2510173 
                 0 
                 7 
                 TLL2 
                 tolloid-like 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11844] 
               
               
                 ENSG00000134256 
                 −1.3577477 
                 0 
                 7 
                 CD101 
                 CD101 molecule 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5949] 
               
               
                 ENSG00000272463 
                 −1.1413394 
                 0 
                 7 
                 RP11-532F6.3 
               
               
                 ENSG00000102794 
                 −1.5942464 
                 0 
                 7 
                 IRG1 
                 immunoresponsive 1 
               
               
                   
                   
                   
                   
                   
                 homolog (mouse) 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33904] 
               
               
                 ENSG00000223799 
                 −1.6341444 
                 0 
                 7 
                 IL10RB-AS1 
                 IL10RB antisense RNA 1 
               
               
                   
                   
                   
                   
                   
                 (head to head) 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 44303] 
               
               
                 ENSG00000019582 
                 −1.1055825 
                 0 
                 7 
                 CD74 
                 CD74 molecule, major 
               
               
                   
                   
                   
                   
                   
                 histocompatibility complex, 
               
               
                   
                   
                   
                   
                   
                 class II invariant chain 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1697] 
               
               
                 ENSG00000121577 
                 −1.2357302 
                 0 
                 7 
                 POPDC2 
                 popeye domain containing 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17648] 
               
               
                 ENSG00000215268 
                 −1.7373145 
                 0 
                 7 
                 LA16c-60G3.8 
               
               
                 ENSG00000119121 
                 −1.4785767 
                 0 
                 7 
                 TRPM6 
                 transient receptor potential 
               
               
                   
                   
                   
                   
                   
                 cation channel, subfamily M, 
               
               
                   
                   
                   
                   
                   
                 member 6 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17995] 
               
               
                 ENSG00000108576 
                 −1.2299055 
                 0 
                 7 
                 SLC6A4 
                 solute carrier family 6 
               
               
                   
                   
                   
                   
                   
                 (neurotransmitter 
               
               
                   
                   
                   
                   
                   
                 transporter), member 4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11050] 
               
               
                 ENSG00000274818 
                 −1.8854991 
                 0 
                 7 
                 RP1-292L20.3 
               
               
                 ENSG00000198133 
                 −1.9389698 
                 0 
                 7 
                 TMEM229B 
                 transmembrane protein 229B 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20130] 
               
               
                 ENSG00000130477 
                 −1.2541053 
                 0 
                 7 
                 UNC13A 
                 unc-13 homolog A 
               
               
                   
                   
                   
                   
                   
                 ( C. elegans ) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23150] 
               
               
                 ENSG00000266094 
                 −1.0579637 
                 0 
                 7 
                 RASSF5 
                 Ras association 
               
               
                   
                   
                   
                   
                   
                 (RalGDS/AF-6) domain 
               
               
                   
                   
                   
                   
                   
                 family member 5 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17609] 
               
               
                 ENSG00000137571 
                 −1.0904993 
                 0 
                 7 
                 SLCO5A1 
                 solute carrier organic anion 
               
               
                   
                   
                   
                   
                   
                 transporter family, member 
               
               
                   
                   
                   
                   
                   
                 5A1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 19046] 
               
               
                 ENSG00000272512 
                 −1.5915514 
                 0 
                 7 
                 RP11-54O7.17 
               
               
                 ENSG00000124391 
                 −1.7278326 
                 0 
                 7 
                 IL17C 
                 interleukin 17C 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5983] 
               
               
                 ENSG00000136052 
                 −1.3272223 
                 0 
                 7 
                 SLC41A2 
                 solute carrier family 41 
               
               
                   
                   
                   
                   
                   
                 (magnesium transporter), 
               
               
                   
                   
                   
                   
                   
                 member 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 31045] 
               
               
                 ENSG00000185245 
                 −1.9464332 
                 0 
                 7 
                 GP1BA 
                 glycoprotein Ib (platelet), 
               
               
                   
                   
                   
                   
                   
                 alpha polypeptide 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4439] 
               
               
                 ENSG00000203685 
                 −1.8061183 
                 0 
                 7 
                 C1orf95 
                 chromosome 1 open reading 
               
               
                   
                   
                   
                   
                   
                 frame 95 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30491] 
               
               
                 ENSG00000149654 
                 −1.331613 
                 0 
                 7 
                 CDH22 
                 cadherin 22, type 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 13251] 
               
               
                 ENSG00000230943 
                 −1.574129 
                 0 
                 7 
                 RP11-367G18.1 
               
               
                 ENSG00000215277 
                 −3.2333936 
                 0 
                 7 
                 RNF212B 
                 ring finger protein 212B 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20438] 
               
               
                 ENSG00000112139 
                 −1.2861961 
                 0 
                 7 
                 MDGA1 
                 MAM domain containing 
               
               
                   
                   
                   
                   
                   
                 glycosylphosphatidylinositol 
               
               
                   
                   
                   
                   
                   
                 anchor 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 19267] 
               
               
                 ENSG00000143494 
                 −1.5135205 
                 0 
                 7 
                 VASH2 
                 vasohibin 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 25723] 
               
               
                 ENSG00000151883 
                 −1.1760751 
                 0 
                 7 
                 PARP8 
                 poly (ADP-ribose) 
               
               
                   
                   
                   
                   
                   
                 polymerase family, member 
               
               
                   
                   
                   
                   
                   
                 8 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 26124] 
               
               
                 ENSG00000136514 
                 −1.9750242 
                 0 
                 7 
                 RTP4 
                 receptor (chemosensory) 
               
               
                   
                   
                   
                   
                   
                 transporter protein 4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23992] 
               
               
                 ENSG00000106258 
                 −1.012592 
                 0 
                 7 
                 CYP3A5 
                 cytochrome P450, family 3, 
               
               
                   
                   
                   
                   
                   
                 subfamily A, polypeptide 5 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 2638] 
               
               
                 ENSG00000243649 
                 −2.5551714 
                 0 
                 7 
                 CFB 
                 complement factor B 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1037] 
               
               
                 ENSG00000164342 
                 −1.0290951 
                 0 
                 7 
                 TLR3 
                 toll-like receptor 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11849] 
               
               
                 ENSG00000115956 
                 −2.3479537 
                 0 
                 7 
                 PLEK 
                 pleckstrin [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 9070] 
               
               
                 ENSG00000144476 
                 −1.7184658 
                 0 
                 7 
                 ACKR3 
                 atypical chemokine receptor 
               
               
                   
                   
                   
                   
                   
                 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 23692] 
               
               
                 ENSG00000157601 
                 −1.4173764 
                 0 
                 7 
                 MX1 
                 MX dynamin-like GTPase 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7532] 
               
               
                 ENSG00000177409 
                 −1.1465499 
                 0 
                 7 
                 SAMD9L 
                 sterile alpha motif domain 
               
               
                   
                   
                   
                   
                   
                 containing 9-like 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1349] 
               
               
                 ENSG00000119917 
                 −1.8565474 
                 0 
                 7 
                 IFIT3 
                 interferon-induced protein 
               
               
                   
                   
                   
                   
                   
                 with tetratricopeptide repeats 
               
               
                   
                   
                   
                   
                   
                 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5411] 
               
               
                 ENSG00000271503 
                 −1.7683442 
                 0 
                 7 
                 CCL5 
                 chemokine (C-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 5 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10632] 
               
               
                 ENSG00000117226 
                 −1.1447048 
                 0 
                 7 
                 GBP3 
                 guanylate binding protein 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4184] 
               
               
                 ENSG00000163840 
                 −1.3311379 
                 0 
                 7 
                 DTX3L 
                 deltex 3 like, E3 ubiquitin 
               
               
                   
                   
                   
                   
                   
                 ligase [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30323] 
               
               
                 ENSG00000010030 
                 −1.2207673 
                 0 
                 7 
                 ETV7 
                 ets variant 7 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18160] 
               
               
                 ENSG00000261884 
                 −1.3310986 
                 0 
                 7 
                 CTC-479C5.12 
                 Uncharacterized protein 
               
               
                   
                   
                   
                   
                   
                 {ECO: 0000313|Ensembl: 
               
               
                   
                   
                   
                   
                   
                 ENSP00000463376} 
               
               
                   
                   
                   
                   
                   
                 [Source: UniProtKB/TrEMBL; 
               
               
                   
                   
                   
                   
                   
                 Acc: J3QL48] 
               
               
                 ENSG00000152229 
                 −1.0179291 
                 0 
                 7 
                 PSTPIP2 
                 proline-serine-threonine 
               
               
                   
                   
                   
                   
                   
                 phosphatase interacting 
               
               
                   
                   
                   
                   
                   
                 protein 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 9581] 
               
               
                 ENSG00000100678 
                 −2.0514071 
                 0 
                 7 
                 SLC8A3 
                 solute carrier family 8 
               
               
                   
                   
                   
                   
                   
                 (sodium/calcium exchanger), 
               
               
                   
                   
                   
                   
                   
                 member 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11070] 
               
               
                 ENSG00000225194 
                 −2.4201688 
                 0 
                 7 
                 LINC00092 
                 long intergenic non-protein 
               
               
                   
                   
                   
                   
                   
                 coding RNA 92 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 31408] 
               
               
                 ENSG00000140968 
                 −1.1898419 
                 0 
                 7 
                 IRF8 
                 interferon regulatory factor 8 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5358] 
               
               
                 ENSG00000006210 
                 −1.2182721 
                 0 
                 7 
                 CX3CL1 
                 chemokine (C-X3-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10647] 
               
               
                 ENSG00000221963 
                 −1.1392138 
                 0 
                 7 
                 APOL6 
                 apolipoprotein L, 6 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14870] 
               
               
                 ENSG00000130589 
                 −1.0673283 
                 0 
                 7 
                 HELZ2 
                 helicase with zinc finger 2, 
               
               
                   
                   
                   
                   
                   
                 transcriptional coactivator 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30021] 
               
               
                 ENSG00000239713 
                 −1.6622438 
                 0 
                 7 
                 APOBEC3G 
                 apolipoprotein B mRNA 
               
               
                   
                   
                   
                   
                   
                 editing enzyme, catalytic 
               
               
                   
                   
                   
                   
                   
                 polypeptide-like 3G 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17357] 
               
               
                 ENSG00000151023 
                 −1.0139189 
                 0 
                 7 
                 ENKUR 
                 enkurin, TRPC channel 
               
               
                   
                   
                   
                   
                   
                 interacting protein 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 28388] 
               
               
                 ENSG00000187123 
                 −1.3193979 
                 0 
                 7 
                 LYPD6 
                 LY6/PLAUR domain 
               
               
                   
                   
                   
                   
                   
                 containing 6 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 28751] 
               
               
                 ENSG00000253831 
                 −3.0507137 
                 0 
                 7 
                 ETV3L 
                 ets variant 3-like 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33834] 
               
               
                 ENSG00000246130 
                 −3.0351799 
                 0 
                 7 
                 RP11-875O11.2 
               
               
                 ENSG00000128335 
                 −1.8122079 
                 0 
                 7 
                 APOL2 
                 apolipoprotein L, 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 619] 
               
               
                 ENSG00000108702 
                 −4.9466198 
                 0 
                 7 
                 CCL1 
                 chemokine (C-C motif) 
               
               
                   
                   
                   
                   
                   
                 ligand 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 10609] 
               
               
                 ENSG00000105963 
                 −1.2049889 
                 0 
                 7 
                 ADAP1 
                 ArfGAP with dual PH 
               
               
                   
                   
                   
                   
                   
                 domains 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 16486] 
               
               
                 ENSG00000170075 
                 −1.4491156 
                 0 
                 7 
                 GPR37L1 
                 G protein-coupled receptor 
               
               
                   
                   
                   
                   
                   
                 37 like 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14923] 
               
               
                 ENSG00000267607 
                 −1.2021234 
                 0 
                 7 
                 CTD-2369P2.8 
               
               
                 ENSG00000142961 
                 −1.1266143 
                 0 
                 7 
                 MOB3C 
                 MOB kinase activator 3C 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 29800] 
               
               
                 ENSG00000159200 
                 −1.1629133 
                 0 
                 7 
                 RCAN1 
                 regulator of calcineurin 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 3040] 
               
               
                 ENSG00000185291 
                 −1.5031919 
                 0 
                 7 
                 IL3RA 
                 interleukin 3 receptor, alpha 
               
               
                   
                   
                   
                   
                   
                 (low affinity) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6012] 
               
               
                 ENSG00000135917 
                 −1.3131434 
                 0 
                 7 
                 SLC19A3 
                 solute carrier family 19 
               
               
                   
                   
                   
                   
                   
                 (thiamine transporter), 
               
               
                   
                   
                   
                   
                   
                 member 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 16266] 
               
               
                 ENSG00000179817 
                 −1.7239398 
                 0 
                 7 
                 MRGPRX4 
                 MAS-related GPR, member 
               
               
                   
                   
                   
                   
                   
                 X4 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17617] 
               
               
                 ENSG00000173918 
                 −1.1809051 
                 0 
                 7 
                 C1QTNF1 
                 C1q and tumor necrosis 
               
               
                   
                   
                   
                   
                   
                 factor related protein 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14324] 
               
               
                 ENSG00000198879 
                 −1.3578392 
                 0 
                 7 
                 SFMBT2 
                 Scm-like with four mbt 
               
               
                   
                   
                   
                   
                   
                 domains 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20256] 
               
               
                 ENSG00000272078 
                 −1.1495108 
                 0 
                 7 
                 RP4-734G22.3 
               
               
                 ENSG00000269794 
                 −1.7476941 
                 0 
                 7 
                 AC010642.2 
               
               
                 ENSG00000115919 
                 −1.2162129 
                 0 
                 7 
                 KYNU 
                 kynureninase [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 6469] 
               
               
                 ENSG00000255521 
                 −1.8970116 
                 0 
                 7 
                 RP4-607I7.1 
               
               
                 ENSG00000173193 
                 −1.3401257 
                 0 
                 7 
                 PARP14 
                 poly (ADP-ribose) 
               
               
                   
                   
                   
                   
                   
                 polymerase family, member 
               
               
                   
                   
                   
                   
                   
                 14 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 29232] 
               
               
                 ENSG00000183644 
                 −1.6099016 
                 0 
                 7 
                 C11orf88 
                 chromosome 11 open reading 
               
               
                   
                   
                   
                   
                   
                 frame 88 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 25061] 
               
               
                 ENSG00000253522 
                 −1.2658724 
                 0 
                 7 
                 CTC-231O11.1 
               
               
                 ENSG00000236453 
                 −1.8969845 
                 0 
                 7 
                 AC003092.1 
               
               
                 ENSG00000131979 
                 −1.5762138 
                 0 
                 7 
                 GCH1 
                 GTP cyclohydrolase 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4193] 
               
               
                 ENSG00000069493 
                 −2.1742062 
                 0 
                 7 
                 CLEC2D 
                 C-type lectin domain family 
               
               
                   
                   
                   
                   
                   
                 2, member D [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14351] 
               
               
                 ENSG00000069696 
                 −1.1323561 
                 0 
                 7 
                 DRD4 
                 dopamine receptor D4 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 3025] 
               
               
                 ENSG00000175356 
                 −1.3984803 
                 0 
                 7 
                 SCUBE2 
                 signal peptide, CUB domain, 
               
               
                   
                   
                   
                   
                   
                 EGF-like 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30425] 
               
               
                 ENSG00000128165 
                 −1.1779326 
                 0 
                 7 
                 ADM2 
                 adrenomedullin 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 28898] 
               
               
                 ENSG00000166856 
                 −1.1992337 
                 0 
                 7 
                 GPR182 
                 G protein-coupled receptor 
               
               
                   
                   
                   
                   
                   
                 182 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 13708] 
               
               
                 ENSG00000199161 
                 −1.616849 
                 0 
                 7 
                 MIR126 
                 microRNA 126 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 31508] 
               
               
                 ENSG00000050730 
                 −1.9873165 
                 0 
                 7 
                 TNIP3 
                 TNFAIP3 interacting protein 
               
               
                   
                   
                   
                   
                   
                 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 19315] 
               
               
                 ENSG00000255750 
                 −1.8909724 
                 0 
                 7 
                 RP11-283G6.5 
               
               
                 ENSG00000184530 
                 −2.3505853 
                 0 
                 7 
                 C6orf58 
                 chromosome 6 open reading 
               
               
                   
                   
                   
                   
                   
                 frame 58 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20960] 
               
               
                 ENSG00000104883 
                 −1.3860147 
                 0 
                 7 
                 PEX11G 
                 peroxisomal biogenesis 
               
               
                   
                   
                   
                   
                   
                 factor 11 gamma 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 20208] 
               
               
                 ENSG00000129521 
                 −2.7016498 
                 0 
                 7 
                 EGLN3 
                 egl-9 family hypoxia- 
               
               
                   
                   
                   
                   
                   
                 inducible factor 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14661] 
               
               
                 ENSG00000204482 
                 −1.2475769 
                 0 
                 7 
                 LST1 
                 leukocyte specific transcript 
               
               
                   
                   
                   
                   
                   
                 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14189] 
               
               
                 ENSG00000115267 
                 −1.3445539 
                 0 
                 7 
                 IFIH1 
                 interferon induced with 
               
               
                   
                   
                   
                   
                   
                 helicase C domain 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18873] 
               
               
                 ENSG00000162692 
                 −2.1801821 
                 0 
                 7 
                 VCAM1 
                 vascular cell adhesion 
               
               
                   
                   
                   
                   
                   
                 molecule 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 12663] 
               
               
                 ENSG00000261618 
                 −1.2864344 
                 0 
                 7 
                 RP11-79H23.3 
               
               
                 ENSG00000101276 
                 −1.1705916 
                 0 
                 7 
                 SLC52A3 
                 solute carrier family 52 
               
               
                   
                   
                   
                   
                   
                 (riboflavin transporter), 
               
               
                   
                   
                   
                   
                   
                 member 3 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 16187] 
               
               
                 ENSG00000064309 
                 −1.4651234 
                 0 
                 7 
                 CDON 
                 cell adhesion associated, 
               
               
                   
                   
                   
                   
                   
                 oncogene regulated 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17104] 
               
               
                 ENSG00000167371 
                 −1.4282411 
                 0 
                 7 
                 PRRT2 
                 proline-rich transmembrane 
               
               
                   
                   
                   
                   
                   
                 protein 2 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 30500] 
               
               
                 ENSG00000101017 
                 −1.5583663 
                 0 
                 7 
                 CD40 
                 CD40 molecule, TNF 
               
               
                   
                   
                   
                   
                   
                 receptor superfamily member 
               
               
                   
                   
                   
                   
                   
                 5 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11919] 
               
               
                 ENSG00000164400 
                 0 
                 −1.63408281 
                 8 
                 CSF2 
                 colony stimulating factor 2 
               
               
                   
                   
                   
                   
                   
                 (granulocyte-macrophage) 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 2434] 
               
               
                 ENSG00000172602 
                 0 
                 −1.15027449 
                 8 
                 RND1 
                 Rho family GTPase 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18314] 
               
               
                 ENSG00000174502 
                 0 
                 −2.09273487 
                 8 
                 SLC26A9 
                 solute carrier family 26 
               
               
                   
                   
                   
                   
                   
                 (anion exchanger), member 9 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 14469] 
               
               
                 ENSG00000234290 
                 0 
                 −1.00067832 
                 8 
                 AC116366.6 
               
               
                 ENSG00000170961 
                 0 
                 −2.64619793 
                 8 
                 HAS2 
                 hyaluronan synthase 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4819] 
               
               
                 ENSG00000110848 
                 0 
                 −1.46706866 
                 8 
                 CD69 
                 CD69 molecule 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1694] 
               
               
                 ENSG00000164512 
                 0 
                 −1.20441285 
                 8 
                 ANKRD55 
                 ankyrin repeat domain 55 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 25681] 
               
               
                 ENSG00000167034 
                 0 
                 −1.10541745 
                 8 
                 NKX3-1 
                 NK3 homeobox 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 7838] 
               
               
                 ENSG00000105246 
                 0 
                 −1.39394774 
                 8 
                 EBI3 
                 Epstein-Barr virus induced 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 3129] 
               
               
                 ENSG00000145506 
                 0 
                 −1.41815829 
                 8 
                 NKD2 
                 naked cuticle homolog 2 
               
               
                   
                   
                   
                   
                   
                 ( Drosophila ) [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 17046] 
               
               
                 ENSG00000127533 
                 0 
                 −2.64266235 
                 8 
                 F2RL3 
                 coagulation factor II 
               
               
                   
                   
                   
                   
                   
                 (thrombin) receptor-like 3 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 3540] 
               
               
                 ENSG00000115008 
                 0 
                 −2.15272028 
                 8 
                 IL1A 
                 interleukin 1, alpha 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5991] 
               
               
                 ENSG00000073282 
                 0 
                 −1.31215479 
                 8 
                 TP63 
                 tumor protein p63 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 15979] 
               
               
                 ENSG00000113196 
                 0 
                 −1.61798433 
                 8 
                 HAND1 
                 heart and neural crest 
               
               
                   
                   
                   
                   
                   
                 derivatives expressed 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 4807] 
               
               
                 ENSG00000096996 
                 0 
                 −1.40936482 
                 8 
                 IL12RB1 
                 interleukin 12 receptor, beta 
               
               
                   
                   
                   
                   
                   
                 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5971] 
               
               
                 ENSG00000275582 
                 0 
                 −1.05575947 
                 8 
                 RP4-681N20.5 
               
               
                 ENSG00000244476 
                 0 
                 −1.23492596 
                 8 
                 ERVFRD-1 
                 endogenous retrovirus group 
               
               
                   
                   
                   
                   
                   
                 FRD, member 1 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33823] 
               
               
                 ENSG00000165685 
                 0 
                 −1.10987961 
                 8 
                 TMEM52B 
                 transmembrane protein 52B 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 26438] 
               
               
                 ENSG00000172331 
                 0 
                 −1.20450079 
                 8 
                 BPGM 
                 2,3-bisphosphoglycerate 
               
               
                   
                   
                   
                   
                   
                 mutase [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1093] 
               
               
                 ENSG00000198846 
                 0 
                 −1.42254609 
                 8 
                 TOX 
                 thymocyte selection- 
               
               
                   
                   
                   
                   
                   
                 associated high mobility 
               
               
                   
                   
                   
                   
                   
                 group box [Source: GNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 18988] 
               
               
                 ENSG00000258521 
                 0 
                 −1.0229865 
                 8 
                 RP11-638I2.9 
               
               
                 ENSG00000279133 
                 0 
                 −1.46999903 
                 8 
                 RP11-342K2.1 
               
               
                 ENSG00000121905 
                 0 
                 −2.21994573 
                 8 
                 HPCA 
                 hippocalcin [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 5144] 
               
               
                 ENSG00000232810 
                 0 
                 −1.4782116 
                 8 
                 TNF 
                 tumor necrosis factor 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 11892] 
               
               
                 ENSG00000178882 
                 0 
                 −1.71201963 
                 8 
                 FAM101A 
                 family with sequence 
               
               
                   
                   
                   
                   
                   
                 similarity 101, member A 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 27051] 
               
               
                 ENSG00000173391 
                 0 
                 −1.01327133 
                 8 
                 OLR1 
                 oxidized low density 
               
               
                   
                   
                   
                   
                   
                 lipoprotein (lectin-like) 
               
               
                   
                   
                   
                   
                   
                 receptor 1 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 8133] 
               
               
                 ENSG00000257671 
                 0 
                 −1.03664909 
                 8 
                 RP3-416H24.1 
               
               
                 ENSG00000269826 
                 0 
                 −1.64046441 
                 8 
                 RP11-158I3.3 
               
               
                 ENSG00000176907 
                 0 
                 −1.2182476 
                 8 
                 C8orf4 
                 chromosome 8 open reading 
               
               
                   
                   
                   
                   
                   
                 frame 4 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 1357] 
               
               
                 ENSG00000165478 
                 0 
                 −1.21164831 
                 8 
                 HEPACAM 
                 hepatic and glial cell 
               
               
                   
                   
                   
                   
                   
                 adhesion molecule 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 26361] 
               
               
                 ENSG00000175746 
                 0 
                 −1.57742953 
                 8 
                 C15orf54 
                 chromosome 15 open reading 
               
               
                   
                   
                   
                   
                   
                 frame 54 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 33797] 
               
               
                 ENSG00000187848 
                 0 
                 −2.24446361 
                 8 
                 P2RX2 
                 purinergic receptor P2X, 
               
               
                   
                   
                   
                   
                   
                 ligand gated ion channel, 2 
               
               
                   
                   
                   
                   
                   
                 [Source: HGNC 
               
               
                   
                   
                   
                   
                   
                 Symbol; Acc: HGNC: 15459] 
               
               
                   
               
               
                 List of genes modified by SB203580 or UM101 (see also FIG. 6); Bin number refers to gene expression response pattern: 
               
               
                 1 = increased expression with both inhibitors 
               
               
                 5 = increased with UM101, unchanged with SB203580 
               
               
                 2 = decreased with UM101, increased with SB203580 
               
               
                 6 = unchanged with UM101, increased with SB203580 
               
               
                 3 = decreased expression with both inhibitors 
               
               
                 7 = decreased with UM101, unchanged with SB203580 
               
               
                 4 = increased with UM101, decreased with SB203580 
               
               
                 8 = unchanged with UM101, decreased with SB203580 
               
            
           
         
       
     
     SB203580 inhibited expression of 61 TNFα-induced genes, 28 of which were also inhibited by UM101 (Table 6, Table 5,  FIG.  5   ). SB203580 increased expression of 38 genes, 10 of which were also increased by UM101. Of the 28 genes inhibited by both SB203580 and UM101, 22 coded for known proteins, including IL-1B, CCL17, MMP9, IDO1, CXCL5, 10 and 11, hyaluronan synthase-3, MUC4, and PLA2 (Table 6). Of the 33 genes inhibited by SB203580 but not UM101, 24 coded for known proteins, including GM-CSF, IL-1α, TNFα, IL-12 receptor-β1, and hyaluronan synthase-2 (Table 6). 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Effect of SB203580 and UM101 in HMVECLs on TNFα-induced genes  1   
               
            
           
           
               
               
               
               
            
               
                 Gene 
                   
                 LOG fold-change 
                 LOG fold-change 
               
               
                 Symbol 
                 Gene name 
                 SB203580 vs. DMSO 
                 UM101 vs. DMSO 
               
               
                   
               
            
           
           
               
            
               
                 Genes inhibited by both SB203580 and UM101 
               
            
           
           
               
               
               
               
            
               
                 PRRG4 
                 proline rich Gla 4 
                 −1.782580534 
                 −1.198488233 
               
               
                 TSLP 
                 thymic stromal lymphopoietin 
                 −1.651439594 
                 −1.336652511 
               
               
                 CCL17 
                 chemokine (C-C motif) ligand 17 
                 −1.834143455 
                 −2.730309773 
               
               
                 EXOC3L4 
                 exocyst complex component 3-like 4 
                 −1.479163179 
                 −1.160471021 
               
               
                 MMP9 
                 matrix metallopeptidase 9 
                 −1.157091348 
                 −1.091179627 
               
               
                 IDO1 
                 indoleamine 2,3-dioxygenase 1 
                 −3.510632932 
                 −3.567987354 
               
               
                 CXCL10 
                 chemokine (C-X-C motif) ligand 10 
                 −3.100915562 
                 −4.369836708 
               
               
                 CD200 
                 CD200 
                 −1.729285649 
                 −1.538155406 
               
               
                 SLC15A3 
                 solute carrier family 15, member 3 
                 −1.00338842 
                 −1.73105887 
               
               
                 VDR 
                 Vitamin D receptor 
                 −1.16718631 
                 −1.19731694 
               
               
                 IL1B 
                 Interleukin-1β 
                 −1.401586926 
                 −1.172530543 
               
               
                 GPR88 
                 G protein-coupled receptor 88 
                 −1.397083754 
                 −2.150599176 
               
               
                 CD207 
                 CD207 (langerin) 
                 −1.547757288 
                 −3.382437255 
               
               
                 TCHH 
                 trichohyalin 
                 −1.504958085 
                 −1.547665316 
               
               
                 HAS3 
                 hyaluronan synthase 3 
                 −1.43377734 
                 −1.124339564 
               
               
                 GBP1P1 
                 guanylate binding protein 1 
                 −1.363287203 
                 −1.755706078 
               
               
                 MUC4 
                 Mucin-4 
                 −2.859491876 
                 −1.057315692 
               
               
                 ELOVL7 
                 ELOVL fatty acid elongase 7 
                 −1.340933369 
                 −1.381063226 
               
               
                 CXCL11 
                 chemokine (C-X-C motif) ligand 11 
                 −1.377905942 
                 −4.136354868 
               
               
                 GBP4 
                 guanylate binding protein 4 
                 −1.259283076 
                 −2.835947907 
               
               
                 PLA1A 
                 phospholipase A1 member A 
                 −1.27452433 
                 −1.500633356 
               
               
                 CXCL5 
                 chemokine (C-X-C motif) ligand 5 
                 −1.017427849 
                 −1.468307731 
               
            
           
           
               
            
               
                 Genes inhibited by SB203580 but not UM101 
               
            
           
           
               
               
               
               
            
               
                 CSF2 
                 GM-CSF 
                 −1.634082807 
                      ns  2   
               
               
                 RND1 
                 Rho family GTPase 1 
                 −1.15027449 
                 ns 
               
               
                 SLC26A9 
                 solute carrier family 26, member 9 
                 −2.092734866 
                 ns 
               
               
                 HAS2 
                 hyaluronan synthase 2 
                 −2.646197932 
                 ns 
               
               
                 CD69 
                 CD69 
                 −1.467068659 
                 ns 
               
               
                 ANKRD55 
                 ankyrin repeat domain 55 
                 −1.204412851 
                 ns 
               
               
                 NKX3-1 
                 NK3 homeobox 1 
                 −1.105417452 
                 ns 
               
               
                 EBI3 
                 Epstein-Barr virus induced 3 
                 −1.393947741 
                 ns 
               
               
                 NKD2 
                 naked cuticle homolog 2 
                 −1.418158287 
                 ns 
               
               
                 F2RL3 
                 coagulation factor II receptor-like 3 
                 −2.642662346 
                 ns 
               
               
                 IL1A 
                 Interleukin-1alpha 
                 −2.152720278 
                 ns 
               
               
                 TP63 
                 Tumor protein 63 
                 −1.312154792 
                 ns 
               
               
                 HAND1 
                 heart and neural crest derivatives 
                 −1.617984328 
                 ns 
               
               
                   
                 expressed 1 
               
               
                 IL12RB1 
                 interleukin 12 receptor, beta 1 
                 −1.409364824 
                 ns 
               
               
                 ERVFRD-1 
                 endogenous retrovirus group FRD, 
                 −1.234925956 
                 ns 
               
               
                   
                 member 1 
               
               
                 TMEM52B 
                 transmembrane protein 52B 
                 −1.109879612 
                 ns 
               
               
                 BPGM 
                 2,3-bisphosphoglycerate mutase 
                 −1.204500786 
                 ns 
               
               
                 TOX 
                 thymocyte selection-associated high 
                 −1.422546092 
                 ns 
               
               
                   
                 mobility group box 
               
               
                 HPCA 
                 hippocalcin 
                 −2.219945733 
                 ns 
               
               
                 TNF 
                 Tumor necrosis factor-alpha 
                 −1.478211598 
                 ns 
               
               
                 FAM101A 
                 family with sequence similarity 101, 
                 −1.712019627 
                 ns 
               
               
                   
                 member A 
               
               
                 OLR1 
                 oxidized low density lipoprotein receptor 
                 −1.013271327 
                 ns 
               
               
                   
                 1 
               
               
                 HEPACAM 
                 hepatic and glial cell adhesion molecule 
                 −1.211648309 
                 ns 
               
               
                 P2RX2 
                 purinergic receptor P2X 
                 −2.244463614 
                 ns 
               
               
                   
               
               
                   1  HMVECLs were preincubated with either 0.4% DMSO, 10 μM SB20350, or 100 μM UM101 for 1 h, then stimulated with 10 ng/ml TNFα for 4 h and RNASeq performed. 
               
               
                   2  not significant 
               
            
           
         
       
     
     The differentially expressed genes were further analyzed using PathwayNet and Ingenuity™ tools to identify the transcription factors and biological pathways regulated by the two inhibitors. PathwayNet analysis suggested that UM101 inhibits some of the SB203580-inhibited transcription factors (Stat-1, c-Fos, c-Jun, NFκB, p53, PPARγ, and Sp1), but not others (ATF1, ATF2, Elk1, c/EBPβ, USF1, SMAD3, FOXO1, and CREB via MSK1/2). Ingenuity™ analysis suggested that both SB203580 and UM101 inhibit the Dendritic Cell Maturation, Triggering Receptor Expressed on Myeloid cells-1 (TREM1), High Mobility Group Box 1 (HMGB1), and NFκB pathways and both increase Liver X-Receptor/Retinoid X-Receptor (LXR/RXR) activation, while only SB203580 inhibits IL-6, Acute Phase, and Cholecystokinin/Gastrin-mediated pathways ( FIG.  3   a   ). UM101 at 100 μM reduced expression of 115 genes and increased expression of 119 genes that were not modified by SB203580 (Table 5), which Ingenuity™ pathway analysis suggested reduced Toll-like receptor and Wnt/β-catenin signaling and increased Nitric Oxide in Cardiovascular Disease pathways ( FIG.  3   b   ). 
     Example 6: Comparing Effects of SB203580 and UM101 on p38 MAPK Substrate Phosphorylation Profile 
     To assess whether UM101 selectively inhibits phosphorylation consistent with its target, HeLa cells were pretreated for 30 min with 10 μM SB203580, 50 μM UM101, or 0.1% DMSO vehicle control, then with the p38 activator, anisomycin (25 μg/ml) and phosphorylated MK2, and Stat-1 were analyzed by immunoblotting ( FIG.  3   c   ). Anisomycin-stimulated phosphorylation of MK2 and Stat-1 were reduced by both 10 μM SB203580 and 50 μM UM1010, but more so with SB203580. 
     Example 7: Analyzing Specific Binding of UM101 to p38α 
     DSF was used to analyze concentration-specific binding of UM101 to p38α and p38ß. While SB203580 stabilized both p38α and p38β, UM101 only stabilized p38α ( FIG.  3   d   ). To confirm that UM101 bound the CADD-targeted pocket, DSF was used to compare UM101- and SB203580-binding to wild-type p38α and a p38α mutant with four of the ten target pocket amino acids (R49K/HL 107-8TF/K165R) substituted ( FIG.  3   e   ). The mutant exhibited SB203580-binding that was identical to wild-type p38α, but no UM101-binding. 
     Selective binding of UM101 to the CADD-targeted pocket in p38α was confirmed using Saturation Transfer Difference (STD)-NMR. A 1D spectrum of UM101 in the presence of p38α is shown in  FIG.  3   f    and the STD spectrum of the same sample is shown in  FIG.  3   g   . The peaks in the 1D spectrum are labeled according to tentative peak assignments of UM101 in aqueous form, based on assignments of UM101 in 2 mM d6-DMSO, which were obtained from the use of 1D proton and C13 and 2D-HMBC experiments. The shifts of the peaks in the STD spectrum correspond well to those of the 1D spectrum, thus indicating that protons in both aromatic rings of UM101 interact with p38α. In contrast, while the 1D spectra for UM101 with p38β and mutated p38α were similar to that of UM101/p38α ( FIG.  3   h    and  FIG.  3   j   ), the interaction of UM101 with p38β and mutated p38α is much weaker, as indicated by the barely discernible peaks of the aromatic protons in the STD spectrum of UM101 with p38β ( FIG.  3   i   ) and mutated p38α ( FIG.  3   k   ). 
     Example 8: Synthetic Methods for Preparing Exemplary Compounds of the Disclosure 
     General Methods for Chemistry: All air or moisture sensitive reactions were performed under positive pressure of nitrogen with oven-dried glassware. Chemical reagents and anhydrous solvents are obtained from commercial sources and used as-is. 
     The p38α MAPK inhibitors of the disclosure can be prepared by methods generally known in the art. For example, compound UM101 can be prepared as depicted in Scheme 1. UM101 can be prepared in two steps from three commercially available fragments (Scheme 1), which facilitates its optimization. Acylation of 4-aminobenzaldehyde with 4-chlorobenzoyl chloride in the presence of diisopropylamine (DIPEA) generates an intermediate aldehyde. Subsequent reductive amination of the aldehyde with thiomorpholine 1,1-dioxide and Na-triacetoxyborohydride (NaBH(OAc) 3 ) affords UM101. 
     
       
         
         
             
             
         
       
     
     A focused structure-activity relationship (SAR) of UM101 and additional lead compounds is conducted to determine its pharmacophore and the information used to achieve its optimization, for example by feeding this information back into the CADD model to improve its predictability thereby facilitating subsequent design cycles. A summary of the proposed modifications to UM101 is shown in Scheme 2, which is driven by the SILCS molecular modeling, and addresses improvements in binding affinity and specificity, and enhancements in physicochemical properties. Importantly, the STD-NMR analysis of UM101 confirmed that both its aromatic rings interact with the protein, hence modification of these rings will impact binding affinities. First, since the positively-charged (under physiological conditions) piperidine-type nitrogen of UM101 is predicted to interact with negatively-charged residues such as D112 and D168, will be retained in some embodiments. According to the FragMaps, aliphatic (e.g., cyclohexyl) or aromatic (e.g. furan) substituents off the central phenyl ring should enhance binding to the protein via interactions with V30, V38, A51, I84, L108, and L167. In addition, the presence of hydrogen bond acceptor maps overlapping with the aliphatic maps suggest that combined aliphatic/hydrogen bond acceptor groups, such as OEt, are incorporated ortho to the aniline nitrogen in some embodiments. Hydrogen bond donor (e.g. NH 2 , OH) and/or acceptor groups (e.g. OMe, isoxazole) are incorporated into the ortho and meta positions of the peripheral chlorophenyl ring in some embodiments, which may be replaced with a chloropyridyl ring in other embodiments. These changes further increase compound solubility. Although the chlorine of the chlorophenyl ring interacts modestly with the protein as judged by SILCS GFE analysis in some embodiments, this site is also varied with alternative hydrophobic and more polar groups in other embodiments. The trans-amide bond is modified to a rigid E-alkene in some embodiments, and a more flexible sulfonamide in other embodiments. The sulfone S02 group contributes −0.5 kcal/mol to binding based on SILCS GFE analysis, indicating that this region of the molecule can be exploited to optimize the molecule&#39;s physicochemical properties without compromising binding affinity in some embodiments. For example, the S02 group is replaced with a polar oxygen atom in some embodiments, and also an NMe group in other embodiments. 
     
       
         
         
             
             
         
       
     
     Example 9: Novel Non-ATP/Catalytic Site p38 Mitogen Activated Protein Kinase Inhibitors 
     Computer-aided drug design (CADD) was used to identify novel small molecule compounds that target select substrate-binding sites and inhibit p38 mitogen activated protein (MAP) kinase activity that contributes to acute lung injury. These compounds are unique in that they are NON-ATP/catalytic site kinase inhibitors and provide an alternative to the current p38 MAP kinase inhibitors that target ATP binding or catalytic sites and block all enzyme activity. Compound UM101 targets the p38α MAP kinase isoform and is the major p38 MAP kinase isoform associated with chronic inflammation and tissue damage associated with acute lung injury. Current p38 MAP kinase inhibitors target the ATP binding or catalytic site, which blocks all enzyme activity, and lack isoform selectivity. In contrast, the compounds disclosed herein target sites that are unique to protein substrate interactions and block specific p38α MAP kinase functions. 
     Novel UM101 analogues were synthesized (Schemes 3-5) and tested for interactions with p38α MAP kinase and biological activity. 
     
       
         
         
             
             
         
       
     
     
       
         
         
             
             
         
       
     
     
       
         
         
             
             
         
       
     
     Two lines of evidence suggest that the lead compounds can improve lung barrier function and prevent lung injury. First, the compounds can block thrombin-induced cell permeability in human vascular endothelial cells ( FIG.  8   ). Second, mice challenged with LPS to induce lung inflammation had reduced protein leak into the bronchoalveolar lavage fluid in the presence of the parent compound UM101 and analogues SF-6-222 and SF-7-009 ( FIG.  9   ), or analogues SF-6-222, SF-7-009, and SF-7-044 ( FIG.  10   ). As shown in  FIG.  9   , UM101 and analogues inhibit acute lung injury in a mouse model. Mice were pretreated i.p. with 1 mg of test compounds and then treated with 100 mg LPS i.t. at 39° C. to induce inflammation. Lung injury was assessed by measuring total protein in the lung lavage fluid. As shown in  FIG.  10   , UM101 and analogues inhibit acute lung injury in a mouse model. All mice received 50 μg LPS i.t. and were kept 24 h at 39° C.; all treatments 1 mg in 0.5 ml DMSO given 6 h post-LPS; mean±SM, n=5, * p=0.03 vs DMSO #1; † p=0.16 vs DMSO #2. Lung injury was assessed by measuring total protein in the lung lavage fluid. 
     The useful application of this disclosure is to provide new therapies for acute lung injury and other diseases caused by inflammation and endothelial permeability. In addition, the disclosure is aimed at overcoming issues with toxicity and poor efficacy with the current p38 MAP kinase inhibitors used in the clinic to treat inflammation. 
     A number of patent and non-patent publications are cited herein in order to describe the state of the art to which this disclosure pertains. The entire disclosure of each of these publications is incorporated by reference herein. 
     While certain embodiments of the present disclosure have been described and/or exemplified above, various other embodiments will be apparent to those skilled in the art from the foregoing disclosure. The present disclosure is, therefore, not limited to the particular embodiments described and/or exemplified, but is capable of considerable variation and modification without departure from the scope and spirit of the appended claims. 
     Moreover, as used herein, the term “about” means that amounts, sizes, formulations, parameters, shapes and other quantities and characteristics are not and need not be exact, but may be approximate and/or larger or smaller, as desired, reflecting tolerances, conversion factors, rounding off, measurement error and the like, and other factors known to those of skill in the art. In general, an amount, size, formulation, parameter, shape or other quantity or characteristic is “about” or “approximate” whether or not expressly stated to be such. 
     Furthermore, the transitional terms “comprising”, “consisting essentially of” and “consisting of”, when used in the appended claims, in original and amended form, define the claim scope with respect to what unrecited additional claim elements or steps, if any, are excluded from the scope of the claim(s). The term “comprising” is intended to be inclusive or open-ended and does not exclude any additional, unrecited element, method, step or material. The term “consisting of” excludes any element, step or material other than those specified in the claim and, in the latter instance, impurities ordinary associated with the specified material(s). The term “consisting essentially of” limits the scope of a claim to the specified elements, steps or material(s) and those that do not materially affect the basic and novel characteristic(s) of the claimed disclosure. All compounds, compositions, formulations, and methods described herein that embody the present disclosure can, in alternate embodiments, be more specifically defined by any of the transitional terms “comprising,” “consisting essentially of,” and “consisting of.” 
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