Patent Publication Number: US-2016237120-A1

Title: Polyomavirus peptide sequences

Description:
The current invention relates to the identification of B-cell epitopes (as linear peptides) from human polyoma virus proteins and their use in an immune diagnostic assay. 
     Progressive multifocal leukoencephalopathy (PML) is a rare but often fatal brain disease caused by reactivation of the polyomavirus JC. The monoclonal antibodies natalizumab, efalizumab, and rituximab—used for the treatment of multiple sclerosis, psoriasis, hematological malignancies, Crohn&#39;s disease, and rheumatic diseases—have been associated with PML. Worldwide 181 (as of November 2011) cases of natalizumab-associated PML have been reported. International studies and standardization of methods are urgently needed to devise strategies to mitigate the risk of PML in natalizumab-treated patients. 
     A new set of assay developments could lead to a better understanding of the virus reactivation, and that could lead to safe use of immune modulating agents (e.g. a Tysabri® (natalizumab)) and an optimized treatment algorithm. 
     BACKGROUND 
     The human neurotropic polyomavirus JCV is a non-enveloped DNA virus belonging to the group of polyomaviruses. JCV is the etiologic agent of progressive multifocal leukoencephalopathy (PML). Other members of this viral family are BK virus (mainly infecting the kidneys), and the non-human SV40 virus. JC and BK viruses have been named using the initials of the first patients discovered with the diseases. 
     Epidemiological studies showed that in certain populations, the seroprevalence of close to 90% by age 20. In those healthy immunocompetent individuals, JCV is establishing a lifelong sub-clinical infection. 
     The initial site of infection may be the tonsils, or possibly the gastrointestinal tract. The virus remains latent and/or can infect the tubular epithelial cells in the kidneys where it continues to reproduce, thereby shedding virus particles in the urine. JCV can cross the blood-brain barrier, and enters into the central nervous system where it infects oligodendrocytes and astrocytes. 
     Immunodeficiency or immuno-suppression allows JCV to reactivate. In the brain, this will cause the usually fatal PML by destroying oligodendrocytes. 
     Therefore, PML is a demyleating disease affecting the white matter, but is in process different from multiple sclerosis (MS), in which the myelin itself is destroyed. Whether the process behind PML is caused by the reactivation of JCV within the CNS or seeding of newly reactivated JCV via blood or lymphatics is unknown. PML progresses much more quickly than MS. 
     There are case reports of PML being induced by pharmacological agents (efalizumab, rituximab, infliximab, natalizumab . . . ) but the process how JCV interacts with these mAbs and cause PML is again not clearly understood. 
     PML is diagnosed by testing for JC virus DNA in cerebrosinal fluid, or in brain biopsy specimens. In addition, brain damage caused by PML has been detected on MRI images. 
     As of today, there is no known cure for PML, but the disease can be slowed or stopped, dependent on improvement of the patient&#39;s immune restoration (e.g. HAART in AIDS patients). A rare complication of immune reconstitution is known as “immune reconstitution inflammatory syndrome (IRIS), in which increased immune system activity increases the damage caused by the infection. IRIS can be managed by pharmacological intervention, but it is extremely fatal if it occurs in PML. 
     Access to Clinical Isolates 
     In order to study the correlates of JCV and PML, a large collection of clinical samples is needed, inclusive with the individual&#39;s clinical background. 
     JCV replicates in several different types of tissues (tonsils, gastro-intestinal tract, kidney, brain). In order to obtain a representative set of genetic variants and the corresponding serological markers, it is aimed to start with the collection of a large sample set from urine, blood, CSF, bone marrow, and paraffin embedded brain biopsy material, and potentially tonsil biopsy. Blood cells can be separated into different compartments (FACS). PML is a rare disease present only in immune suppressed individuals, and access to these precious materials is foreseen to be limited. Most of the study objectives for assay design can be completed on samples from infected healthy individuals. 
     The Genetic Variability of JCV (Genotypes and Variants) and Tropism 
     Sequencing of the JCV genome indicates at least seven major genotypes and numerous subtypes. The type distribution was found to be as follows: Type 1: in Europeans; Types 2 and 7: in Asians; Types 3 and 6: in Africans; Type 4: in the United States, the whole genome of Type 4 strains was found to be most closely related to Type 1; and Type 5: a single natural occurring recombinant strain of Type 6 in VP1 gene with Type 2B in the early region. These genotypes and subtypes have been defined in three ways: namely by i) a 610 bp region spanning the 3′ ends of the VP1 and T-antigen genes, ii) a 215 bp region of the 5′ end of the VP1 gene and iii) based on the sequence of the entire coding region of the genome (5130 bp in strain MAD-1, Accession number: PLYCG MAD-1) including untranslated regions except the archetypal regulatory region to the late side of ori. 
     Besides the genotypic variations, the regulatory domain and the VP1 region contains mutations that are found more frequently in PML patients. From the frequency of observation, it is thought that these mutations are positively selected, and are not just present by chance. Analysis of the VP1 sequences isolated from PML patients were compared to control samples from healthy individuals showing that the mutated residues are located within the sialic add binding site, a JC virus receptor for cell infection. It is therefore likely that a more virulent PML-causing phenotype of JC virus is acquired via adaptive evolution that changes viral specificity for its cellular receptor(s). 
     On the other hand, on the basis of the survival time (less or more than 6 months) from the onset of the disease, patients were grouped in slow and fast PML progressors (SP and FP PML). It was suggested that VP1 outer loops can contain polymorphic residues restricted to four positions (aa 74, 75, 117 and 128) in patients with slow PML progression, VP1 loop mutations are associated with a favorable prognosis for PML. 
     The genomic organization and variability of JCV in the transcriptional control region (TCR), a 400 base pare non-coding regulatory region, were described by Jensen (2001). In addition, distinctive point mutations or deletions in the regulatory region also provide useful information to supplement coding region typing. 
     Rearranged JCV regulatory regions (RR), including tandem repeat patterns found in the central nervous system (CNS) of PML patients, have been associated with neurovirulence. 
     In HIV-infected patients with virologically confirmed PML, highly active antiretroviral therapy (HAART) leads to a partial immune-mediated control of JCV replication in CSF. However, the virus may tend to escape through the selection of rearrangements in the RR, some associated with enhanced viral replication efficiency, other resulting in multiplication of binding sites for cellular transcription factors (Macrophage Chemoattractant Protein MCP-1, cellular transcription factor NF-1). In a case of PML in an HIV-1 infected individual that did not respond to HAART therapy, there was a simultaneous presence of JCV strains with four different TCR structures in urine, peripheral blood cells, serum, and CSF samples, for which the authors suggested that the archetype TCR is restricted to urine, while the degree of the rearrangement varies and increases from the peripheral blood to CSF. 
     It is currently not clear if PML is more frequently found within certain genotypes, or if certain genotypes are excluded from PML. Also the genetic polymorphisms in VP1 and the RR need further analysis in the context of the different genotypes, tissue distribution, and presence/absence of PML. 
     While infection is very common in most human populations, this is usually subclinical since the virus is readily controlled by the immune system. After the initial infection is resolved, JCV nonetheless persists in the body and enters a state of latency which is poorly understood. However, under circumstances in which the immune system becomes impaired, e.g., AIDS, the virus reactivates and replicates in the central nervous system (CNS) to cause PML. The mechanisms involved in this reactivation are not known but it is possible that changes in the levels of cytokines and immunomodulators, such as TNF-α, MIP-1α and TGF-β, that are associated with immunosuppression, elicit changes in intracellular signal transduction pathways that, in turn, modulate the activities of transcription factors (e.g. Sp1 and Egr-1) that bound to the GG(A/C)-rich sequences in the TCR. These transcription factors are involved in regulating the expression of JCV genes. 
     JCV DNA is frequently, but intermittently detected in peripheral blood, supporting the hypothesis of viral reservoirs. In addition, mRNAs were seldom associated with DNA, suggesting that JCV reactivation does not take place in peripheral blood. JCV might remain latent in the peripheral reservoir, and immune suppression might enable reactivation, thereby facilitating the detection of JCV DNA in blood. However, circulating virus might have no link to the emergence of PML. 
     JCV Natural History 
     Antibody titers to JCV were measured in the past with hemagglutination inhibition (HI) assays. Nowadays, hemagglutination- and HI-assays are only used to study modifications in Vp1 and the effect of these mutations on receptor recognition. HI assays are replaced by antibody detection technologies. The detected antibodies to JCV are against Vp1 epitopes, the protein that makes up 75% of the total virion protein. 
     Recently, in addition to the previously characterized viruses BK and JO, three new human polyomaviruses have been identified: KIV (respiratory tract infection), WUV (respiratory tract infection), and MCV (merkel cell carcinoma). It was determined that initial exposure to KIV, WUV, and MCV occurs in childhood, similar to that for the known human polyomaviruses BKV and JCV, and that their prevalence is high. In order to study exposure to these viruses in humans, recombinant polyomavirus VP1 capsid proteins were expressed in  E. coli  in an ELISA assay. 
     Sera of 1501 adult individuals were tested for the presence of 7 polyomaviruses (including SV40=primate virus, in humans through the SV40-contaminated polio vaccine: and LPV=lymphotropic polyoma virus in African green monkeys) and the authors indicated that there may be an age-related waning of BKV VP1 specific antibodies, but not for the other 6 polyomaviruses tested. Also, a difference in sero-prevalence with respect to gender for any of the 7 polyomaviruses tested was not found (Kean et al., 2009). Of the 195 samples exhibiting initial SV40 seroreactivity, only 7 (3%) were cross reactive with JCV Vp1 protein. No other cross reactivity with JCV Vp1 was observed. 
     Since there is a causal relationship of reactivation of JCV in CSF and the development of PML, knowing the JCV serological status of individuals with decreased immunological status is crucial. Theoretically, uninfected individuals (seronegative) should not be at risk for developing PML, while seropositive individuals are. There are case reports of PML being caused by pharmacological agents, although there is some speculation this could be due in part to the existing impaired immune response or ‘drug combination therapies’ rather than individual drugs. These include efalizumab, rituximab, belatacept, infliximab, natalizumab, chemotherapy, corticosteroids, and various transplant drugs such as tacrolimus. 
     Epidemiological studies suggest that the JCV infection occurs primarily in childhood, but the infection in adults is not excluded. Seronegative individuals undergoing immunosuppression and/or therapy should in generally not at risk, but they might be in the seroconversion window where antibodies are not yet properly available. Hence this population would require further attention and analysis by molecular diagnostic means. The sensitivity and specificity of a JC virus serology assay is of substantial interest because such an assay is now being considered as a means to assess the risk of PML in patients treated with natalizumab. 
     Current available immune-assays are based on VP1 only, expressed in a baculovirus expression system, in an  E. coli  expression system or in a yeast expression system. No other viral proteins are available in such an assay meaning that only so-called conformational epitopes, but not linear epitopes present in the three dimensional structure of the virus, are part of the immune assay. As a consequence thereof human samples potentially containing antibodies directed against the missing part as such, will not be detected. 
     As a final Tysabri treatment algorithm would require the knowledge of the infection status, there is a high unmet medical need to:
         design serological assays for JCV anti-IgG and anti-IgM, and confirm the serological specificity of the JCV assay against other polyomaviruses.   compare the serological assay results to a ‘gold standard’ molecular assay with detection limit of ˜50 viral copies/ml generating information on sensitivity, specificity, positive and negative predictive values.   convert the serological assay to a point of care technology,   explore the serological status in a large collection of healthy individuals and in different groups of patients.   compare the serology assay with the cellular immune response assay.       

     The current invention therefore relates to human polyoma virus peptide sequences possessing an immune activity towards human antibodies in human samples. 
     More specifically the current invention makes it unexpectedly possible to use the human polyoma viral small T antigen for immune response diagnostic purposes. 
     The 63 specific sequences identified in Table 9 are considered human polyoma viral immune-dominant epitopes as indicated for the several polyoma viruses and can be used for immune diagnostic purposes accordingly. 
     In addition the human polyoma virus peptide sequences can be used for B-cell epitope studies i.e. the identification of linear peptides present in the three dimensional structure of the virus involved. In addition the human polyoma virus peptide sequences can be used for B-cell stimulation and/or B-cell functionality studies. 
     The human polyoma virus peptide sequences of the invention can also be part of a device or kit further containing means for measuring antibodies in a human test sample, like serum, plasma or whole blood. 
     In addition, the human polyoma virus peptide sequences mentioned in Table 9 can be used, directly or indirectly, for the manufacture of a medicament to treat progressive multifocal leukoencephalopathy (PML). 
    
    
     EXPERIMENTAL SECTION 
     A peptide array representing human polyoma virus proteins has been prepared. The following proteins are covered by the peptide array: agnoprotein, small T antigen, large T antigen, VP1, VP2, VP3 and VP4 of the viruses BK, JC, KI, WU, MC and SV40. In addition, the VP1 protein of the viruses HPyV6, HPyV7, HPyV9, IPPyV and TSV are also included in this study. In total 4284 15-mer peptides overlapping by 11 residues are displayed in triplicates on one single array chip. 
     In order to prepare the peptide microarrays, polyoma virus protein sequences were retrieved from the NCBI (National Center for Biotechnology) database. The best covering sequence for each of the proteins of each virus was calculated. Then, each sequence was divided in all possible 15-mer peptides and coverage of related sequences by the peptides was calculated. The protein sequence providing the best covering peptides was determined. Mosaic sequences, which further increase the coverage of related sequences, were generated as well. The mosaic algorithm assembles artificial best covering sequences for a given sequence pool. The number of sequences that were retrieved from the NCBI database is given in Table 1 and Table 2. 
     For the design of the 15-mer peptides, the following proteins were included:
         Agnoprotein: 3 best covering sequences, one from each of the viruses BK, JC, SV40 and 6 mosaic sequences   large T antigen: 6 best covering sequences, one from each of the viruses: BK, JC, KI, MC, SV40, WU and 2 mosaic sequences   small T antigen: 6 best covering sequences, one from each of the viruses: BK, JC, KI, MC, SV40, WU and 2 mosaic sequences   VP1: All available sequences from the viruses: BK, JC, KI, MC, SV40, WU, HPyV6, HPyV7, HPyV9, IPPyV and TSV   VP2: 6 best covering sequences, one from each of the viruses: BK, JC, KI, MC, SV40, WU and 2 mosaic sequences   VP3: 6 best covering sequences, one from each of the viruses: BK, JC, KI, MC, SV40, WU and 2 mosaic sequences   VP4: The one available sequence from SV40       

     Clinical Samples Used: 
     A total of 49 plasma samples from healthy volunteers (HV) have been tested on the peptide microarrays. 
     Analysis: 
     Peptides from the microarray that were reactive against antibodies present in the HV plasma samples were aligned against consensus sequences retrieved from the NCBI database. Table 3 provides the accession numbers for the sequences used in the analysis. For analysis purposes, the different proteins for the different organisms were labeled with a unique code (ID). Table 4 gives an overview of these unique identifiers. 
     Results 
     Overview of the Hybridization Results. 
     A total of 49 clinical samples were tested on the peptide microarrays in triplicate (each peptide array contains 3 identical subarrays of 4284 peptides). Data from the subarrays were pooled, and only the median value (in case of 3 valid subarray data points), or the average of 2 data points (in case one of the subarray data points was excluded for quality reasons) were retained for further analysis. This will result in 209,916 data points (4284×49). 
     As a negative control, hybridization buffer without addition of human plasma was run alongside. Analysis of these 4284 control data points showed the following boxplot parameters:
         Minimum=507 fluorescent units (FU; relative measure, equipment dependent)   25th quartile=590 FU   Median=614 FU   75th quartile=642 FU   Maximum=15859 FU       

     For further analysis, the value of the 75th quartile is used as a cut-off, because it is reasonable to assume that from that moment onwards meaningful biological data might be available with the HV samples. 
     The following arbitrary classes of signal intensity were generated and represented in Table 5:
         a. FU signal &gt;642, but &lt;=10,000   b. FU signal &gt;10,000, but &lt;=20,000   c. FU signal &gt;20,000, but &lt;=30,000   d. FU signal &gt;30,000       

     The most important results are found in the FU group of &gt;30,000, with a total of 1,148 data points. However, the presentation of this result does not educate on the number of peptides that are responsible for this hybridization signal. Therefore, a further analysis of these data points was needed (given in Table 6). 
     A total of 635 peptides are responsible for the 1148 data points with an FU value &gt;30,000. The 635 peptides are distributed over different classes of organisms and genes, with strong response to small T antigen peptides being the most prevalent for KIV, WUV, MCV, and JCV, followed by large T antigen and VP1, and a strong signal is the least prevalently found in VP2, VP3, and Agnoprotein. The sequence of these 635 peptides is given in Table 19. For interpretation of the origin of the peptides see Table 20 
     IDs given in table 19 which are not defined in table 20 do not represent further specified polyoma virus peptide sequences. 
     Immunodominancy 
     Subsequently, an analysis towards the immuno-dominancy of these peptides was conducted. Therefore, for each of the 4284 peptides the number of hits was searched for with a FU of &gt;10,000 in each of the 49 HV samples. 
     The analysis retrieved the following result: 2424 peptides had at least “one out of the 49” HV samples a FU-value &gt;10,000. As a consequence, 1860 peptides were having FU values below the arbitrary cut-off of 10,000 for all the samples tested (Note: this does not mean that for certain disease states these peptides might not show reaction with available antibodies). In addition, subgroups of prevalence were defined in blocks of 5 HV (Table 7). For the purpose of this exercise, we considered reaction on a peptide as immunodominant from &gt;21 reactions (out of 49 HV) onwards. 
     A total of 63 peptides were identified for which the label of immunodominant epitope would be applicable (according to the above assumptions) (Table 8). The sequence of these 63 immuno dominant peptides is given in Table 9. 
     Detection of Peptides with Average FU Values &gt;10000 Across the 49 HV 
     The dataset of 209,916 data points was analyzed for average values per peptide. This means that for each peptide, the average of FU values was calculated across the 49 HV reaction patterns. A total of 106 peptides were retrieved with values &gt;10,000. The distribution of these peptides per organism is given in Table 10. In Table 11 to 18 the peptide sequences per organism are given. 
     Summary 
     Peptide arrays (15-mer peptides) were prepared covering all proteins of human polyoma viruses including BK virus, JC virus, KI virus, WU virus, MC virus, SV40, HPyV6, HPyV7, HPyV9, IPPyV and TSV. 
     Serum samples from 49 healthy volunteers were tested for the presence of antibodies against these peptides. As a result a set of potential B-cell epitopes were identified as described above. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Number of protein sequences retrieved from the  
               
               
                 NCBI database for the indicated viruses 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 agno 
                 large T 
                 small T 
                 VP1 
                 VP2 
                 VP3 
                 VP4 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 BKV 
                 305 
                 381 
                 339 
                 1338 
                 295 
                 289 
                   
               
               
                 JCV 
                 710 
                 1993 
                 638 
                 2481 
                 642 
                 638 
                   
               
               
                 KIV 
                   
                 13 
                 30 
                 53 
                 12 
                 9 
                   
               
               
                 MCV 
                   
                 110 
                 65 
                 60 
                 34 
                 11 
                   
               
               
                 SV40 
                 51 
                 149 
                 53 
                 60 
                 51 
                 29 
                 1 
               
               
                 WUV 
                   
                 90 
                 85 
                 84 
                 223 
                 73 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Number of protein sequences retrieved from  
               
               
                 NCBI database for other polyoma viruses 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 agno 
                 large T 
                 small T 
                 VP1 
                 VP2 
                 VP3 
                 VP4 
               
               
                   
               
               
                 HPyV6 
                   
                 7 
                 7 
                 7 
                 7 
                 7 
                   
               
               
                 HPyV7 
                   
                 7 
                 7 
                 7 
                 7 
                 7 
                   
               
               
                 HPyV9 
                   
                 2 
                 2 
                 2 
                 2 
                 2 
                   
               
               
                 IPPyV 
                   
                 1 
                 1 
                 1 
                 1 
                 1 
                   
               
               
                 TSV 
                   
                 2 
                 2 
                 2 
                 2 
                 2 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 NCBI database accession numbers for polyomavirus proteins used in 
               
               
                 the peptide analysis. 
               
            
           
           
               
               
            
               
                   
                 ACCESSION NUMBER  
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 BKV 
                 JCV 
                 KIV 
                 MCV 
                 SV40 
                 WUV 
                 HPyV6 
                 HPyV7 
               
               
                   
               
               
                 VP1 
                 CAA24299 
                 AAA82101 
                 ACB12026 
                 AEM01098 
                 YP_003708381  
                 ACB12036 
                 YP_003848918 
                 YP_003848923 
               
               
                 VP2 
                   
                 AAA82099 
                 ACB12024 
                 AEM01099 
                 YP_003708379 
                 ACB12034 
                   
                   
               
               
                 large T 
                 CAA24300 
                 AAA82102 
                 ACB12028 
                 AEM01097 
                 YP_003708382 
                 ACB12038 
                   
                   
               
               
                 small T 
                 CAA24301 
                 AAA82103 
                 ACB12027 
                 AEM01096 
                 YP_003708383  
                 ACB12037 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Protein and organism identifier (ID) 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 ID 
                 ID 
                 ID 
                 ID 
                 ID 
                 ID 
                 ID 
                 ID 
               
               
                   
                 BKV 
                 JCV 
                 KIV 
                 MCV 
                 SV40 
                 WUV 
                 HPyV6 
                 HPyV7 
               
               
                   
               
               
                 VP1 
                 _1_01 
                 _1_02 
                 _1_03 
                 _1_04 
                 _1_05 
                 _1_06 
                 _1_09 
                 _1_10 
               
               
                 VP2 
                 _2_01 
                 _2_02 
                 _2_03 
                 _2_04 
                 _2_05 
                 _2_06 
                 _2_09 
                 _2_10 
               
               
                 large T 
                 _4_01 
                 _4_02 
                 _4_03 
                 _4_04 
                 _4_05 
                 _4_06 
                 _4_09 
                 _4_10 
               
               
                 small T 
                 _5_01 
                 _5_02 
                 _5_03 
                 _5_04 
                 _5_05 
                 _5_06 
                 _5_09 
                 _5_10 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Overview of the different FU classes per organism and per viral protein. 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 Fluorescent units 
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                 n 
                 &gt;642  
                 &gt;10000 
                 &gt;20000 
                   
                   
               
               
                 Organism 
                 gene 
                 ID 
                 peptides 
                 &lt;10000 
                 &lt;20000 
                 &lt;30000 
                 &gt;30000 
                 total 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 other 
                 0_05 
                 3 
                 146 
                 — 
                 — 
                 1 
                 147 
               
               
                 other 
                 VP1 
                 1_00 
                 467 
                 22,056 
                 622 
                 112 
                 93 
                 22,883 
               
               
                 BKV 
                 VP1 
                 1_01 
                 423 
                 20,146 
                 461 
                 60 
                 60 
                 20,727 
               
               
                 JCV 
                 VP1 
                 1_02 
                 758 
                 35,160 
                 1,518 
                 264 
                 200 
                 37,142 
               
               
                 KIV 
                 VP1 
                 1_03 
                 69 
                 3,295 
                 70 
                 8 
                 8 
                 3,381 
               
               
                 MCV 
                 VP1 
                 1_04 
                 165 
                 7,774 
                 246 
                 34 
                 31 
                 8,085 
               
               
                 SV40 
                 VP1 
                 1_05 
                 69 
                 3,300 
                 59 
                 9 
                 13 
                 3,381 
               
               
                 WUV 
                 VP1 
                 1_06 
                 83 
                 3,980 
                 69 
                 11 
                 7 
                 4,067 
               
               
                 IPPyV 
                 VP1 
                 1_07 
                 89 
                 4,147 
                 166 
                 26 
                 22 
                 4,361 
               
               
                 TSV 
                 VP1 
                 1_08 
                 89 
                 4,105 
                 180 
                 39 
                 37 
                 4,361 
               
               
                 HPyV6 
                 VP1 
                 1_09 
                 97 
                 4,482 
                 180 
                 40 
                 51 
                 4,753 
               
               
                 HPyV7 
                 VP1 
                 1_10 
                 115 
                 5,391 
                 191 
                 31 
                 22 
                 5,635 
               
               
                 BKV 
                 VP2 
                 2_01 
                 81 
                 3,860 
                 91 
                 10 
                 8 
                 3,969 
               
               
                 JCV 
                 VP2 
                 2_02 
                 71 
                 3,276 
                 155 
                 19 
                 29 
                 3,479 
               
               
                 KIV 
                 VP2 
                 2_03 
                 96 
                 4,564 
                 99 
                 22 
                 19 
                 4,704 
               
               
                 MCV 
                 VP2 
                 2_04 
                 57 
                 2,703 
                 66 
                 9 
                 15 
                 2,793 
               
               
                 SV40 
                 VP2 
                 2_05 
                 74 
                 3,473 
                 119 
                 20 
                 14 
                 3,626 
               
               
                 WUV 
                 VP2 
                 2_06 
                 92 
                 4,356 
                 105 
                 23 
                 24 
                 4,508 
               
               
                 mosaic 
                 VP2 
                 2_12 
                 68 
                 3,200 
                 65 
                 19 
                 48 
                 3,332 
               
               
                 JCV 
                 VP3 
                 3_02 
                 3 
                 147 
                 — 
                 — 
                 — 
                 147 
               
               
                 MCV 
                 VP3 
                 3_04 
                 6 
                 292 
                 1 
                 — 
                 1 
                 294 
               
               
                 BKV 
                 large T 
                 4_01 
                 162 
                 7,624 
                 223 
                 54 
                 37 
                 7,938 
               
               
                 JCV 
                 large T 
                 4_02 
                 136 
                 6,323 
                 277 
                 36 
                 28 
                 6,664 
               
               
                 KIV 
                 large T 
                 4_03 
                 157 
                 7,194 
                 374 
                 70 
                 55 
                 7,693 
               
               
                 MCV 
                 large T 
                 4_04 
                 202 
                 9,423 
                 345 
                 64 
                 66 
                 9,898 
               
               
                 SV40 
                 large T 
                 4_05 
                 155 
                 7,119 
                 357 
                 60 
                 59 
                 7,595 
               
               
                 WUV 
                 large T 
                 4_06 
                 155 
                 7,040 
                 384 
                 96 
                 75 
                 7,595 
               
               
                 mosaic 
                 large T 
                 4_12 
                 75 
                 3,487 
                 149 
                 20 
                 19 
                 3,675 
               
               
                 BKV 
                 small T 
                 5_01 
                 21 
                 925 
                 83 
                 13 
                 8 
                 1,029 
               
               
                 7CV 
                 small T 
                 5_02 
                 22 
                 935 
                 103 
                 25 
                 15 
                 1,078 
               
               
                 KIV 
                 small T 
                 5_03 
                 27 
                 1,068 
                 202 
                 35 
                 18 
                 1,323 
               
               
                 MCV 
                 small T 
                 5_04  
                 27 
                 1,163 
                 124 
                 24 
                 12 
                 1,323 
               
               
                 SV40 
                 small T 
                 5_05 
                 24 
                 1,067 
                 90 
                 14 
                 5 
                 1,176 
               
               
                 WUV 
                 small T 
                 5_06 
                 28 
                 1,153 
                 167 
                 35 
                 17 
                 1,372 
               
               
                 mosaic 
                 small T 
                 5_12 
                 24 
                 939 
                 170 
                 42 
                 25 
                 1,176 
               
               
                 BKV 
                 agno 
                 6_01 
                 13 
                 624 
                 10 
                 1 
                 2 
                 637 
               
               
                 JCV 
                 agno 
                 6_02 
                 15 
                 725 
                 9 
                 1 
                 — 
                 735 
               
               
                 SV40 
                 agno 
                 6_05 
                 13 
                 611 
                 25 
                 — 
                 1 
                 637 
               
               
                 mosaic 
                 agno 
                 6_12  
                 53 
                 2,561 
                 26 
                 7 
                 3 
                 2,597 
               
               
                   
                   
                 TOTAL 
                 4284 
                 199,834 
                 7,581 
                 1,353 
                 1,148 
                 209,916 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Identification of organism_gene peptides with FU value &gt;30,000. 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                 number of 
                 n peptides 
                 % peptides 
               
               
                   
                   
                   
                   
                 hits with 
                 with 
                 with 
               
               
                   
                   
                   
                 n 
                 FU value 
                 FU value 
                 FU value  
               
               
                 organism  
                 gene 
                 ID 
                 peptides 
                 &gt;30000 
                 &gt;30000 
                 &gt;30000 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 JCV 
                 VP3 
                 3_02 
                 3 
                 0 
                 0 
                 0 
               
               
                 JCV 
                 agno 
                 6_02 
                 15 
                 0 
                 0 
                 0 
               
               
                 BKV 
                 VP2 
                 2_01 
                 81 
                 8 
                 4 
                 5 
               
               
                 mosaic 
                 agno 
                 6_12 
                 53 
                 3 
                 3 
                 6 
               
               
                 WUV 
                 VP1 
                 1_06  
                 83 
                 7 
                 5 
                 6 
               
               
                 SV40 
                 agno 
                 6_05 
                 13 
                 1 
                 1 
                 8 
               
               
                 SV40 
                 VP1 
                 1_05 
                 69 
                 13 
                 6 
                 9 
               
               
                 BKV 
                 VP1 
                 1_01 
                 423 
                 60 
                 40 
                 9 
               
               
                 HPyV7 
                 VP1 
                 1_10 
                 115 
                 22 
                 11 
                 10 
               
               
                 KIV 
                 VP1 
                 1_03 
                 69 
                 8 
                 7 
                 10 
               
               
                 MCV 
                 VP2 
                 2_04  
                 57 
                 15 
                 6 
                 11 
               
               
                 mosaic 
                 VP2 
                 2_12 
                 68 
                 48 
                 8 
                 12 
               
               
                 SV40 
                 VP2 
                 2_05 
                 74 
                 14 
                 9 
                 12 
               
               
                 other 
                 VP1 
                 1_00  
                 467 
                 93 
                 57 
                 12 
               
               
                 KIV 
                 VP2 
                 2_03 
                 96 
                 19 
                 13 
                 14 
               
               
                 JCV 
                 VP2 
                 2_02 
                 71 
                 29 
                 10 
                 14 
               
               
                 MCV 
                 large T 
                 4_04  
                 202 
                 66 
                 29 
                 14 
               
               
                 MCV 
                 VP1 
                 1_04  
                 165 
                 31 
                 25 
                 15 
               
               
                 JCV 
                 VP1 
                 1_02  
                 758 
                 200 
                 116 
                 15 
               
               
                 BKV 
                 agno 
                 6_01 
                 13 
                 2 
                 2 
                 15 
               
               
                 BKV 
                 large T 
                 4_01  
                 162 
                 37 
                 25 
                 15 
               
               
                 JCV 
                 large T 
                 4_02  
                 136 
                 28 
                 21 
                 15 
               
               
                 WUV 
                 VP2 
                 2_06  
                 92 
                 24 
                 15 
                 16 
               
               
                 MCV 
                 VP3 
                 3_04  
                 6 
                 1 
                 1 
                 17 
               
               
                 SV40 
                 small T 
                 5_05 
                 24 
                 5 
                 4 
                 17 
               
               
                 IPPyV 
                 VP1 
                 1_07 
                 89 
                 22 
                 15 
                 17 
               
               
                 HPyV6 
                 VP1 
                 1_09 
                 97 
                 51 
                 18 
                 19 
               
               
                 mosaic 
                 large T 
                 4_12  
                 75 
                 19 
                 14 
                 19 
               
               
                 BKV 
                 small T 
                 5_01  
                 21 
                 8 
                 4 
                 19 
               
               
                 KIV 
                 large T 
                 4_03  
                 157 
                 55 
                 30 
                 19 
               
               
                 SV40 
                 large T 
                 4_05  
                 155 
                 59 
                 31 
                 20 
               
               
                 WUV 
                 large T 
                 4_06 
                 155 
                 75 
                 35 
                 23 
               
               
                 JCV 
                 small T 
                 5_02 
                 22 
                 15 
                 5 
                 23 
               
               
                 TSV 
                 VP1 
                 1_08 
                 89 
                 37 
                 26 
                 29 
               
               
                 MCV 
                 small T 
                 5_04  
                 27 
                 12 
                 8 
                 30 
               
               
                 WUV 
                 small T 
                 5_06  
                 28 
                 17 
                 9 
                 32 
               
               
                   
                 other 
                 0_05  
                 3 
                 1 
                 1 
                 33 
               
               
                 KIV 
                 small T 
                 5_03 
                 27 
                 18 
                 10 
                 37 
               
               
                 mosaic 
                 small T 
                 5_12 
                 24 
                 25 
                 11 
                 46 
               
               
                   
                   
                   
                   
                 1148 
                 635 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Detection of immunodominant epitopes. 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                   
                   
                 Total 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 total 
               
               
                   
                   
                   
                 Pep- 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 &gt;1 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 tides  
                 number of HV samples that show reactivity 
                 sample 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 Organ- 
                   
                   
                 in 
                   
                   
                 &gt;6 
                   &gt;11 
                   &gt;16 
                   &gt;21 
                   &gt;26 
                   &gt;31 
                   &gt;36 
                   &gt;41 
                   &gt;46 
                 reac- 
               
               
                 ism 
                 gene 
                 ID 
                 class 
                 0 
                 &lt;=5 
                 &lt;=10 
                 &lt;=15 
                 &lt;=20 
                 &lt;=25 
                 &lt;=30 
                 &lt;=35 
                 &lt;=40 
                 &lt;=45 
                 &lt;=49 
                 tive 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 other 
                 0_05 
                 3 
                 2 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 1 
               
               
                 other  
                 VP1 
                 1_00 
                 467 
                 245 
                 168 
                 38 
                 8 
                 4 
                 2 
                 0 
                 1 
                 0 
                 1 
                 0 
                 222 
               
               
                 BKV 
                 VP1 
                 1_01 
                 423 
                 206 
                 190 
                 20 
                 5 
                 0 
                 1 
                 0 
                 0 
                 1 
                 0 
                 0 
                 217 
               
               
                 JCV 
                 VP1 
                 1_02 
                 758  
                 327 
                 301 
                 81 
                 22  
                 15 
                 10  
                 1 
                 0 
                 1 
                 0 
                 0 
                 431 
               
               
                 KIV 
                 VP1 
                 1_03 
                 69 
                 33  
                 33 
                 2 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 36 
               
               
                 MCV 
                 VP1 
                 1_04 
                 165  
                 78  
                 65 
                 16 
                 5 
                 0 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 87 
               
               
                 SV40 
                 VP1 
                 1_05 
                 69 
                 41 
                 23 
                 4 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 28 
               
               
                 WUV 
                 VP1 
                 1_06 
                 83 
                 43 
                 37 
                 1 
                 2 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 40 
               
               
                 IPPyV 
                 VP1 
                 1_07 
                 89 
                 20  
                 53 
                 13 
                 2 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 69 
               
               
                 TSV 
                 VP1 
                 1_08 
                 89 
                 18  
                 53 
                 14 
                 3 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 71 
               
               
                 HPyV6  
                 VP1 
                 1_09 
                 97 
                 35 
                 46 
                 10 
                 2 
                 2 
                 2 
                 0 
                 0 
                 0 
                 0 
                 0 
                 62 
               
               
                 HPyV7  
                 VP1 
                 1_10 
                 115  
                 48  
                 51 
                 12 
                 1 
                 2 
                 0 
                 1 
                 0 
                 0 
                 0 
                 0 
                 67 
               
               
                 BKV 
                 VP2 
                 2_01 
                 81 
                 41  
                 34 
                 4 
                 2 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 40 
               
               
                 JCV 
                 VP2 
                 2_02 
                 71 
                 32 
                 25 
                 7 
                 3 
                 3 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 39 
               
               
                 KIV 
                 VP2 
                 2_03 
                 96 
                 58 
                 29 
                 6 
                 1 
                 1 
                 0 
                 1 
                 0 
                 0 
                 0 
                 0 
                 38 
               
               
                 MCV 
                 VP2 
                 2_04 
                 57 
                 36  
                 13 
                 6 
                 1 
                 0 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 21 
               
               
                 SV40 
                 VP2 
                 2_05 
                 74 
                 41 
                 22 
                 8 
                 1 
                 1 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 33 
               
               
                 WUV 
                 VP2 
                 2_06 
                 92 
                 43 
                 42 
                 6 
                 0 
                 0 
                 0 
                 0 
                 1 
                 0 
                 0 
                 0 
                 49 
               
               
                 mosaic 
                 VP2 
                 2_12 
                 68 
                 33  
                 28 
                 3 
                 1 
                 3 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 35 
               
               
                 JCV 
                 VP3 
                 3_02 
                 3 
                 3 
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
               
               
                 MCV 
                 VP3 
                 3_04 
                 6 
                 4 
                 2 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 2 
               
               
                 BKV 
                 large T 
                 4_01 
                 162  
                 67 
                 70 
                 20 
                 3 
                 0 
                 1 
                 0 
                 1 
                 0 
                 0 
                 0 
                 95 
               
               
                 JCV 
                 large T  
                 4_02 
                 136  
                 50  
                 64 
                 14 
                 3 
                 4 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 86 
               
               
                 KIV 
                 large T 
                 4_03 
                 157  
                 49 
                 78 
                 19 
                 5 
                 2 
                 1 
                 2 
                 0 
                 0 
                 1 
                 0 
                 108 
               
               
                 MCV 
                 large T  
                 4_04 
                 202 
                 74 
                 99 
                 19 
                 6 
                 2 
                 1 
                 0 
                 0 
                 0 
                 0 
                 1 
                 128 
               
               
                 SV40 
                 large T 
                 4_05 
                 155  
                 63  
                 61 
                 18 
                 3 
                 5 
                 3 
                 2 
                 0 
                 0 
                 0 
                 0 
                 92 
               
               
                 WUV 
                 large T  
                 4_06 
                 155 
                 54 
                 71 
                 18 
                 2 
                 3 
                 2 
                 2 
                 1 
                 1 
                 1 
                 0 
                 101 
               
               
                 mosaic 
                 large T 
                 4_12 
                 75 
                 27  
                 39 
                 4 
                 2 
                 3 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 48 
               
               
                 BKV 
                 small T  
                 5_01 
                 21 
                 6 
                 5 
                 6 
                 3 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 15 
               
               
                 JCV 
                 small T 
                 5_02 
                 22 
                 7 
                 6 
                 4 
                 2 
                 2 
                 0 
                 0 
                 0 
                 1 
                 0 
                 0 
                 15 
               
               
                 KIV 
                 small T 
                 5_03 
                 27 
                 4 
                 8 
                 6 
                 3 
                 2 
                 0 
                 2 
                 1 
                 1 
                 0 
                 0 
                 23 
               
               
                 MCV 
                 small T 
                 5_04 
                 27 
                 8 
                 8 
                 6 
                 2 
                 0 
                 1 
                 2 
                 0 
                 0 
                 0 
                 0 
                 19 
               
               
                 SV40 
                 small T 
                 5_05 
                 24 
                 3 
                 12 
                 5 
                 3 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 21 
               
               
                 WUV 
                 small T 
                 5_06 
                 28 
                 2 
                 15 
                 3 
                 3 
                 1 
                 2 
                 1 
                 0 
                 0 
                 0 
                 1 
                 26 
               
               
                 mosaic 
                 small T 
                 5_12 
                 24 
                 2 
                 5 
                 6 
                 4 
                 4 
                 2 
                 1 
                 0 
                 0 
                 0 
                 0 
                 22 
               
               
                 BKV 
                 agno 
                 6_01 
                 13 
                 9 
                 3 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 4 
               
               
                 JCV 
                 agno 
                 6_02 
                 15 
                 9 
                 6 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 6 
               
               
                 SV40 
                 agno 
                 6_05 
                 13 
                 5 
                 6 
                 2 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 8 
               
               
                 mosaic 
                 agno 
                 6_12 
                 53 
                 34 
                 18 
                 1 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 19 
               
               
                   
                   
                 To- 
                   
                   
                 1790 
                 403 
                 105 
                 63 
                 33 
                 15 
                 5 
                 5 
                 3 
                 2 
                 2424 
               
               
                   
                   
                 tal 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Distribution of 63 immuno dominant peptides 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 BKV 
                 JCV 
                 KIV 
                 MCV 
                 SV40 
                 WUV  
                 HPyV6  
                 HPyV7 
                 mosaic 
                 else 
                 total 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 VP1 
                 2 
                 12 
                   
                 1 
                   
                   
                 2 
                 1 
                   
                 4 
                 22 
               
               
                 VP2 
                   
                 1 
                 1 
                 1 
                 1 
                 1 
                   
                   
                   
                   
                 5 
               
               
                 large T 
                 2 
                 1 
                 4 
                 2 
                 5 
                 7 
                   
                   
                   
                   
                 21 
               
               
                 small T 
                   
                 1 
                 4 
                 3 
                   
                 7 
                   
                   
                   
                   
                 15 
               
               
                 total 
                 4 
                 15 
                 9 
                 7 
                 6 
                 15 
                 2 
                 1 
                 0 
                 4 
                 63 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Sequences of the 63 immuno dominant peptides 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 peptide 
                 peptide 
               
               
                 ID 
                 organism 
                 gene 
                 number 
                 sequence 
               
               
                   
               
               
                 4_01 
                 BKV 
                 large T 
                 3118 
                 LDSEISMYTFSRMKY 
               
               
                   
               
               
                 4_01 
                 BKV 
                 large T 
                 3119 
                 ISMYTFSRMKYNICM 
               
               
                   
               
               
                 4_02 
                 JCV 
                 large T 
                 3244 
                 TMNEYSVPRTLQARF 
               
               
                   
               
               
                 4_03 
                 KIV 
                 large T 
                 3332 
                 KGVNNPYGLYSRMCR 
               
               
                   
               
               
                 4_03 
                 KIV 
                 large T 
                 3295 
                 IPTYGTPDWDEWWSQ 
               
               
                   
               
               
                 4_03 
                 KIV 
                 large T 
                 3279 
                 MSCWGNLPLMRRQYL 
               
               
                   
               
               
                 4_03 
                 KIV 
                 large T 
                 3333 
                 NPYGLYSRMCRQPFN 
               
               
                   
               
               
                 4_04 
                 MCV 
                 large T 
                 3546 
                 LAHYLDFAKPFPCQK 
               
               
                   
               
               
                 4_04 
                 MCV 
                 large T 
                 3532 
                 MPEMYNNLCKPPYKL 
               
               
                   
               
               
                 4_05 
                 SV40 
                 large T 
                 3637 
                 LGLERSAWGNIPLMR 
               
               
                   
               
               
                 4_05 
                 SV40 
                 large T 
                 3723 
                 MVYNIPKKRYWLFKG 
               
               
                   
               
               
                 4_05 
                 SV40 
                 large T 
                 3638 
                 RSAWGNIPLMRKAYL 
               
               
                   
               
               
                 4_05 
                 SV40 
                 large T 
                 3784 
                 HNQPYHICRGFTCFK 
               
               
                   
               
               
                 4_05 
                 SV40 
                 large T 
                 3653 
                 PTYGTDEWEQWWNAF 
               
               
                   
               
               
                 4_06 
                 WUV 
                 large T 
                 3810 
                 GTPDWDYWWSQFNSY 
               
               
                   
               
               
                 4_06 
                 WUV 
                 large T 
                 3932 
                 LIWCRPVSDFHPCIQ 
               
               
                   
               
               
                 4_06 
                 WUV 
                 large T 
                 3792 
                 LGLDMTCWGNLPLMR 
               
               
                   
               
               
                 4_06 
                 WUV 
                 large T 
                 3919 
                 TMNEYLVPATLAPRF 
               
               
                   
               
               
                 4_06 
                 WUV 
                 large T 
                 3920 
                 YLVPATLAPRFHKTV 
               
               
                   
               
               
                 4_06 
                 WUV 
                 large T 
                 3809 
                 IPTYGTPDWDYWWSQ 
               
               
                   
               
               
                 4_06 
                 WUV 
                 large T 
                 3793 
                 MTCWGNLPLMRTKYL 
               
               
                   
               
               
                 5_02 
                 JCV 
                 small T 
                 4054 
                 IDCYCFDCFRQWFGC 
               
               
                   
               
               
                 5_03 
                 KIV 
                 small T 
                 4079 
                 KPPVWIECYCYKCYR 
               
               
                   
               
               
                 5_03 
                 KIV 
                 small T 
                 4063 
                 QSSQVYCKDLCCNKF 
               
               
                   
               
               
                 5_03 
                 KIV 
                 small T 
                 4075 
                 HCILSKYHKEKYKIY 
               
               
                   
               
               
                 5_03 
                 KIV 
                 small T 
                 4072 
                 CIHGYNHECQCIHCI 
               
               
                   
               
               
                 5_04 
                 MCV 
                 small T 
                 4105 
                 KQKNCLTWGECFCYQ 
               
               
                   
               
               
                 5_04 
                 MCV 
                 small T 
                 4094 
                 DYMQSGYNARFCRGP 
               
               
                   
               
               
                 5_04 
                 MCV 
                 small T 
                 4095 
                 SGYNARFCRGPGCML 
               
               
                   
               
               
                 5_06 
                 WUV 
                 small T 
                 4159 
                 WIECYCYRCYREWFG 
               
               
                   
               
               
                 5_06 
                 WUV 
                 small T 
                 4154 
                 YCFLDKRHKQKYKIF 
               
               
                   
               
               
                 5_06 
                 WUV 
                 small T 
                 4143 
                 ELCCNFPPRKYRLVG 
               
               
                   
               
               
                 5_06 
                 WUV 
                 small T 
                 4158 
                 KPPMWIECYCYRCYR 
               
               
                   
               
               
                 5_12 
                 WUV 
                 small T 
                 4172 
                 FGTWNSSEVSCDFPP 
               
               
                   
               
               
                 5_12 
                 WUV 
                 small T 
                 4187 
                 PLCPDTLYCKDWPIC 
               
               
                   
               
               
                 5_12 
                 WUV 
                 small T 
                 4190 
                 IDCYCFDCFRQWFGL 
               
               
                   
               
               
                 1_01 
                 BKV 
                 VP1 
                  531 
                 EKKMLPCYSTARIPL 
               
               
                   
               
               
                 1_01 
                 BKV 
                 VP1 
                  791 
                 LPCYSTARIPLPNLY 
               
               
                   
               
               
                 1_09 
                 HPyV6 
                 VP1 
                 2306 
                 AAGAANLFGPPVEKQ 
               
               
                   
               
               
                 1_09 
                 HPyV6 
                 VP1 
                 2289 
                 TVDMMFRQFLQPQKP 
               
               
                   
               
               
                 1_10 
                 HPyV7 
                 VP1 
                 2404 
                 ATTGNFQSRGLPYPM 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                  929 
                 DPDMMRYVDRYGQLQ 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                 1576 
                 FNYRTMYPDGTIFPK 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                 1562 
                 FNYRTTYPHGTIFPK 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                  956 
                 GMFTNRCGSQQWRGL 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                 1177 
                 GMFTNRSGFQQWRGL 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                  958 
                 MRYVDRYGQLQTQML 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                  974 
                 PDMMRYVDRYGQSQT 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                  927 
                 PGDPDMMRYVDRYGQ 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                 1338 
                 PNLNEDLTCGNIPMW 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                 1528 
                 YLYKNKAYPVECWVP 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                  926 
                 LPGDPDMMRYVDRYG 
               
               
                   
               
               
                 1_02 
                 JCV 
                 VP1 
                 1427 
                 GMFTNRSCSQQWRGL 
               
               
                   
               
               
                 1_04 
                 MCV 
                 VP1 
                 1817 
                 AKLDKDGNYPIEVWC 
               
               
                   
               
               
                 1_00 
                 other 
                 VP1 
                   99 
                 PDMMRYVDKYGQLQT 
               
               
                   
               
               
                 1_00 
                 other 
                 VP1 
                  352 
                 WVADPSRNDNCRYFG 
               
               
                   
               
               
                 1_00 
                 other 
                 VP1 
                  237 
                 KAYLDKNNAYPVECW 
               
               
                   
               
               
                 1_00 
                 other 
                 VP1 
                  285 
                 PLEMQGVLMNYRTKY 
               
               
                   
               
               
                 2_02 
                 JCV 
                 VP2 
                 2538 
                 AFVNNIHYLDPRHWG 
               
               
                   
               
               
                 2_03 
                 KIV 
                 VP2 
                 2616 
                 YQLETGIPGIPDWLF 
               
               
                   
               
               
                 2_04 
                 MCV 
                 VP2 
                 2707 
                 MAFSLDPLQWENSLL 
               
               
                   
               
               
                 2_05 
                 SV40 
                 VP2 
                 2754 
                 MAVDLYRPDDYYDIL 
               
               
                   
               
               
                 2_06 
                 WUV 
                 VP2 
                 2837 
                 YNLETGIPGVPDWVF 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Distribution of 106 peptides with average signal &gt;10000 
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                   
                 BKV  
                 JCV  
                 KIV  
                 MCV  
                 SV40 
                 WUV 
                 HPyV6 
                 HPyV7 
                 mosaic  
                 else 
                 total 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 VP1 
                 2 
                 20  
                 0 
                 1 
                 1 
                 0 
                 6 
                 2 
                   
                 6 
                 38 
               
               
                 VP2 
                 0 
                 3 
                 2 
                 1 
                 2 
                 1 
                 0 
                 0 
                 4 
                   
                 13 
               
               
                 large T 
                 2 
                 2 
                 5 
                 3 
                 9 
                 9 
                 0 
                 0 
                 1 
                   
                 31 
               
               
                 small T 
                 0 
                 3 
                 5 
                 3 
                 1 
                 7 
                 0 
                 0 
                 5 
                   
                 24 
               
               
                 total 
                 4 
                 28  
                 12  
                 8 
                 13 
                 17  
                 6 
                 2 
                 10 
                 6 
                 106 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Peptides for JCV 
               
            
           
           
               
               
               
               
               
               
            
               
                 ACCESSION 
                   
                 peptide 
                 aa 
                 peptide 
                   
               
               
                 NUMBER 
                   
                 number 
                 position 
                 sequence 
                 alignment 
               
               
                   
               
               
                 AAA82102 
                 Large 
                 3244 
                 528-542 
                 TMNEYSVPRTLQARF 
                   
               
               
                   
                 T-JCV 
                   
                   
                   
                   
               
               
                   
                 Large 
                 3271 
                 666-680 
                 EHCTYHICKGFQCFK 
                   
               
               
                   
                 T-JCV 
                   
                   
                   
                   
               
               
                   
                   
                   
                   
                   
                 FGTWNSSEVGCDFPP 
               
               
                   
               
               
                 AAA82103 
                 Small 
                 4040 
                 74-88 
                 FGTWNSSEVGCDFPP 
                 -------------- 
               
               
                   
                 T-JCV 
                   
                   
                   
                   
               
               
                   
                 Small 
                 4185 
                 74-88 
                 FGTWNSSEVCADFPL 
                 ---------CA---L 
               
               
                   
                 T- 
                   
                   
                   
                   
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                 Small 
                 4172 
                 74-88 
                 FGTWNSSEVSCDFPP 
                 ---------S----- 
               
               
                   
                 T- 
                   
                   
                   
                   
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                   
                   
                   
                   
                 HCPCLMCMLKLRHKNRKFL 
               
               
                   
               
               
                   
                 Small 
                 4174 
                 108-122 
                 HCPCLMCMLKLRHKN 
                 -------------- 
               
               
                   
                 T- 
                   
                   
                   
                   
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                 Small 
                 4049 
                 112-126 
                 LMCMLKLRHRNRKFL 
                 ---------R----- 
               
               
                   
                 T-JCV 
                   
                   
                   
                   
               
               
                   
                 Small 
                 4175 
                 112-126 
                 LMCMLKLRHKNRKFL 
                 -------------- 
               
               
                   
                 T- 
                   
                   
                   
                   
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                   
                   
                   
                   
                 IDCYCFDCFRQWFGC 
               
               
                   
               
               
                   
                 Small 
                 4054 
                 134-148 
                 IDCYCFDCFRQWFGC 
                 -------------- 
               
               
                   
                 T-JCV 
                   
                   
                   
                   
               
               
                   
                 Small 
                 4190 
                 134-148 
                 IDCYCFDCFRQWFGL 
                 -------------L 
               
               
                   
                 T- 
                   
                   
                   
                   
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                 VP1 
                  209 
                 72-86 
                 SPERKMLPCYSTARI 
                   
               
               
                   
                 else 
                   
                   
                   
                   
               
               
                   
               
               
                 AAA82101 
                 VP1-JCV 
                 1338 
                  89-103 
                 PNLNEDLTCGNIPMW 
                   
               
               
                   
                   
                   
                   
                   
                 ALELQGVVCNYRTKYPDGTIFPK 
               
               
                   
                 VP1-JCV 
                 1445 
                 151-165 
                 ALELQGVVCNYRTKY 
                 -------------- 
               
               
                   
                 VP1- 
                  285 
                 151-165 
                 PLEMQGVLMNYRTKY 
                 P--M---LM------ 
               
               
                   
                 else 
                   
                   
                   
                   
               
               
                   
                 (JCV) 
                   
                   
                   
                   
               
               
                   
                 VP1-JCV 
                 1576 
                 159-173 
                 FNYRTMYPDGTIFPK 
                 F----M--------- 
               
               
                   
                 VP1-JCV 
                 1562 
                 159-173 
                 FNYRTTYPHGTIFPK 
                 F----T--H------ 
               
               
                   
                   
                   
                   
                   
                 KAYLDKNNAYPVECWVP 
               
               
                   
                 VP1- 
                  237 
                 187-201 
                 KAYLDKNNAYPVECW 
                   
               
               
                   
                 else 
                   
                   
                   
                   
               
               
                   
                 VP1-JCV 
                 1568 
                 189-203 
                 YLDENKAYPVECWVP 
                 ---E-K---------- 
               
               
                   
                 VP1-JCV  
                 1646 
                 189-203 
                 YLDRNKAYPVECWVP 
                 ---R-K---------- 
               
               
                   
                 VP1-JCV 
                 1528 
                 189-203 
                 YLYKNKAYPVECWVP 
                 --Y--K---------- 
               
               
                   
                 VP1-JCV 
                 1133 
                 234-248 
                 TTVLLDEFGGGPLCK 
                   
               
               
                   
                 VP1-JCV 
                 1146 
                 234-248 
                 TTVLLDEYGVGPLCK 
                   
               
               
                   
                 VP1-JCV 
                 1015 
                 241-255 
                 FGVGPLCKGANLYLS 
                   
               
               
                   
                   
                   
                   
                   
                 GMFTNRCGSQQWRGL 
               
               
                   
                 VP1-JCV 
                  956 
                 261-275 
                 GMFTNRCGSQQWRGL 
                 --------------- 
               
               
                   
                 VP1-JCV 
                 1427 
                 261-275 
                 GMFTNRSCSQQWRGL 
                 ------SC------- 
               
               
                   
                 VP1-JCV 
                 1177 
                 261-275 
                 GMFTNRSGFQQWRGL 
                 ------SGF------ 
               
               
                   
                   
                   
                   
                   
                 LPGDPDMMRYVDRYGQLQTQML 
               
               
                   
                 VP1-JCV 
                  926 
                 333-347 
                 LPGDPDMMRYVDRYG 
                 --------------- 
               
               
                   
                 VP1-JCV 
                  927 
                 334-348 
                 PGDPDMMRYVDRYGQ 
                 --------------- 
               
               
                   
                 VP1-JCV 
                 1649 
                 335-349 
                 GDPDMMRYVDSCRQK 
                 ----------SCR-K 
               
               
                   
                 VP1-JCV 
                  929 
                 336-350 
                 DPDMMRYVDRYGQLQ 
                 --------------- 
               
               
                   
                 VP1- 
                   99 
                 337-351 
                 PDMMRYVDKYGQLQT 
                 --------K------ 
               
               
                   
                 else 
                   
                   
                   
                   
               
               
                   
                 VP1-JCV 
                  974 
                 337-351 
                 PDMMRYVDRYGQSQT 
                 ------------S-- 
               
               
                   
                 VP1-JCV 
                  957 
                 338-352 
                 DMMRYVDRYGQLQTQ 
                 --------------- 
               
               
                   
                 VP1-JCV 
                  958 
                 340-354 
                 MRYVDRYGQLQTQML 
                 --------------- 
               
               
                   
               
               
                 AAA82099 
                 VP2- 
                 2909 
                 116-130 
                 QQPVMALQLFNPEDY 
                   
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                 VP2-3CV 
                 2538 
                 140-154 
                 AFVNNIHYLDPRHWG 
                   
               
               
                   
                   
                   
                   
                   
                 NLVRDDLPSLTSQEIQRRT 
               
               
                   
                 VP2-JCV 
                 2544 
                 167-181 
                 NLVRDDLPSLTSQEI 
                 --------------- 
               
               
                   
                 VP2- 
                 2911 
                 167-181 
                 NLVRDDLPALTSQEI 
                 --------A------ 
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                 VP2- 
                 2940 
                 167-181 
                 NLVRDDLPSLTSREI 
                 ------------R-- 
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                 VP2- 
                 2941 
                 171-185 
                 DDLPSLTSREIQRRT 
                 --------R------ 
               
               
                   
                 mosaic 
                   
                   
                   
                   
               
               
                   
                 VP2-JCV 
                 2572 
                 286-300 
                 ANQRSAPQWMLPLLL 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 Peptides for BKV 
               
            
           
           
               
               
               
               
               
            
               
                 ACC 
                   
                 aa 
                 peptide 
                 peptide sequence 
               
               
                 NUMBERS 
                   
                 gene 
                 position 
                 sequence aligned 
               
               
                   
               
               
                 CAA24300 
                 Large T-BKV 
                 605-619 
                 LDSEISMYTFSRMKY 
                 LDSEISMYTFSRMKY 
               
               
                   
                 Large T-BKV 
                 609-623 
                 ISMYTFSRMKYNICM 
                 -----------NICM 
               
               
                   
                 Large T-mosaic 
                 231-245 
                 EYLLYSALTRDPYYI 
                   
               
               
                   
                 (Bkvirus) 
                   
                   
                   
               
               
                   
               
               
                 CAA24301 
                 Small T-mosaic 
                 74-88 
                 FGTWNSSEVCADFPL 
                 FGTWNSSEVCADFPL 
               
               
                   
                 Small T-mosaic 
                 74-88 
                 FGTWNSSEVSCDFPP 
                 ---------SC---P 
               
               
                   
                 Small T-mosaic 
                 108-122 
                 HCPCLMCMLKLRHKN 
                   
               
               
                   
                 Small T-mosaic 
                 134-148 
                 IDCYCFDCFRQWFGL 
                   
               
               
                   
                   
                   
                   
                 SPERKMLPCYSTARIPLPNLY 
               
               
                   
                 VP1-else (BKV) 
                 80-94 
                 SPERKMLPCYSTARI 
                 --------------- 
               
               
                   
               
               
                 CAA24299 
                 VP1-BKV 
                 82-96 
                 EKKMLPCYSTARIPL 
                 -K------------- 
               
               
                   
                 VP1-BKV 
                 86-100 
                 LPCYSTARIPLPNLY 
                 --------------- 
               
               
                   
                 VP1-else  
                   
                   
                   
               
               
                   
                 (BKV) 
                 195-209 
                 KAYLDKNNAYPVECW 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 Peptidesfor KIV 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 aa 
                   
               
               
                 ACC NUMBERS 
                   
                 position 
                 ID 
               
               
                   
               
               
                 ACB12028 
                 Large T-KIV 
                  21-35 
                 MSCWGNLPLMRRQYL 
               
               
                   
                 Large T-KIV 
                  85-99 
                 IPTYGTPDWDEWWSQ 
               
               
                   
                 Large T-KIV 
                 233-247 
                 KGVNNPYGLYSRMCR 
               
               
                   
                 Large T-KIV 
                 237-251 
                 NPYGLYSRMCRQPFN 
               
               
                   
                 Large T-KIV 
                 269-283 
                 EDLFGEPKEPSLSWN 
               
               
                   
               
               
                 ACB12027 
                 Small T-KIV 
                   
                 CIHGYNHECQCIHCI 
               
               
                   
                 Small T-KIV 
                   
                 HCILSKYHKEKYKIY 
               
               
                   
                 Small T-KIV 
                   
                 KPPVWIECYCYKCYR 
               
               
                   
                 Small T-KIV 
                   
                 QSSQVYCKDLCCNKF 
               
               
                   
                 Small T-KIV 
                   
                 VYCKDLCCNKFRLVG 
               
               
                   
               
               
                 ACB12026 
                 VP1-else 
                 112-126 
                 PDIPNQVSECDMLIW 
               
               
                   
                 (WUV, KIV) 
                   
                   
               
               
                   
                 VP1-else  
                 219-233 
                 WVADPSRNDNCRYFG 
               
               
                   
                 (WUV, KIV) 
                   
                   
               
               
                   
               
               
                 ACB12024 
                 VP2-KIV 
                 317-331 
                 TGGTPHYATPDWILY 
               
               
                   
                 VP2-KIV 
                 152-166 
                 YQLETGIPGIPDWLF 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 14 
               
             
            
               
                   
               
               
                 Peptides for MCV 
               
            
           
           
               
               
               
               
            
               
                 acc number 
                   
                 aa position 
                 ID 
               
               
                   
               
               
                 AEM01097 
                 Large T-MCV 
                 405-419 
                 MPEMYNNLCKPPYKL 
               
               
                   
               
               
                 AEM01097 
                 Large T-MCV 
                 413-427 
                 CKPPYKLLQENKPLL 
               
               
                   
               
               
                 AEM01097 
                 Large T-MCV 
                 461-475 
                 LAHYLDFAKPFPCQK 
               
               
                   
               
               
                 AEM01096 
                 Small T-MCV 
                  93-107 
                 DYMQSGYNARFCRGP 
               
               
                   
               
               
                 AEM01096 
                 Small T-MCV 
                 137-151 
                 KQKNCLTWGECFCYQ 
               
               
                   
               
               
                 AEM01096 
                 Small T-MCV 
                  97-111 
                 SGYNARFCRGPGCML 
               
               
                   
               
               
                 AEM01098 
                 VP1-MCV 
                 218-232 
                 AKLDKDGNYPIEVWC 
               
               
                   
               
               
                 AEM01099 
                 VP2-MCV 
                 129-143 
                 MAFSLDPLQWENSLL 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 15 
               
             
            
               
                   
               
               
                 Peptides for WUV 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 aa 
                   
               
               
                 acc number 
                 gene 
                 position 
                 Sequence 
               
               
                   
               
               
                   
                 Large T-WUV 
                  17-31 
                 LGLDMTCWGNLPLMR 
               
               
                   
               
               
                   
                 Large T-WUV 
                  21-35 
                 MTCWGNLPLMRTKYL 
               
               
                   
               
               
                   
                 Large T-WUV 
                  85-99 
                 IPTYGTPDWDYWWSQ 
               
               
                   
               
               
                 ACB12038 
                 Large T-WUV 
                 89-103 
                 GTPDWDYWWSQFNSY 
               
               
                   
                 Large T-WUV 
                 217-231 
                 PFRHRVSAVNNFCKG 
               
               
                   
                 Large T-WUV 
                 429-443 
                 IVENVPKKRYWVFKG 
               
               
                   
                 Large T-WUV 
                 544-558 
                 TMNEYLVPATLAPRF 
               
               
                   
                 Large T-WUV 
                 548-562 
                 YLVPATLAPRFHKTV 
               
               
                   
                 Large T-WUV 
                 596-610 
                 LIWCRPVSDFHPCIQ 
               
               
                   
                 Small T-WUV 
                  81-95 
                 SSSQVECTELCCNFP 
               
               
                   
                 Small T-WUV 
                  89-103 
                 ELCCNFPPRKYRLVG 
               
               
                   
               
               
                 ACB12037 
                 Small T-WUV 
                 129-143 
                 CNCFYCFLDKRHKQK 
               
               
                   
                 Small T-WUV 
                 133-147 
                 YCFLDKRHKQKYKIF 
               
               
                   
                 Small T-WUV 
                 141-155 
                 KQKYKIFRKPPMWIE 
               
               
                   
                 Small T-WUV 
                 149-163 
                 KPPMWIECYCYRCYR 
               
               
                   
                 Small T-WUV 
                 153-167 
                 WIECYCYRCYREWFG 
               
               
                   
               
               
                 ACB12036 
                 VP1-else 
                 103-117 
                 PDIPNQVSECDMLIW 
               
               
                   
                 (WUV and KIV) 
                 211-225 
                 WVADPSRNDNCRYFG 
               
               
                   
                 VP1-else  
                   
                   
               
               
                   
                 (WUV, and 
                   
                   
               
               
                   
                 others) 
                   
                   
               
               
                   
               
               
                 ACB12034 
                 VP2-WUV 
                 152-166 
                 YNLETGIPGVPDWVF 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 16 
               
             
            
               
                   
               
               
                 Peptides for HPyV6 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 aa 
                 peptide 
                 peptide 
               
               
                 acc number 
                 gene 
                 position 
                 sequence 
                 sequnce aligned 
               
               
                   
               
               
                   
                 VP1-HPyV6 
                  77-91 
                 YTLAWNLPEIPEAL 
                   
               
               
                   
               
               
                   
                 VP1-HPyV6 
                 295-309 
                 TVDMMFRQFLQPQKP 
                 TVDMMFRQFLQPQKP 
               
               
                   
               
               
                   
                 VP1-HPyV6 
                 299-313 
                 MFRQFLQPQKPQVQG 
                 -----------QVQG 
               
               
                   
               
               
                   
                 VP1-HPyV6 
                 363-377 
                 AAGAANLFGPPVEKQ 
                 AAGAANLFGPPVEKQ 
               
               
                   
               
               
                 YP_003848918 
                 VP1-HPyV6 
                 367-381 
                 ANLFGPPVEKQTSKE 
                 -----------TSKE 
               
               
                   
               
               
                   
                 VP1-HPyV6 
                 373-387 
                 PVEKQT5KEP5KGEL 
                 ---------PSKGEL 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 17 
               
             
            
               
                   
               
               
                 Peptides for HPyV7 
               
            
           
           
               
               
               
               
               
            
               
                   
                 peptide 
                 aa 
                   
                   
               
               
                 acc number 
                 number 
                 position 
                   
                 ID 
               
               
                   
               
               
                 YP_ 
                 2404 
                 VP1-HPyV7  
                 51-65 
                 ATTGNFQSRGLPYPM 
               
               
                 003848923 
                   
                   
                   
                   
               
               
                   
               
               
                   
                 2324 
                 VP1-HPyV7  
                 51-65 
                 ATTGNFQSRGLPYTM 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 18 
               
             
            
               
                   
               
               
                 Peptides for SV40 
               
            
           
           
               
               
               
               
               
            
               
                   
                 peptide 
                   
                 aa  
                   
               
               
                 ACC NUMBER 
                 number 
                 gene 
                 position 
                 peptide sequence 
               
               
                   
               
               
                 YP_003708382 
                 3661 
                 largeT-SV40 
                 133-147 
                 EDPKDFPSELLSFLS 
               
               
                   
                 3722 
                 largeT-SV40 
                 408-422 
                 FLKCMVYNIPKKRYW 
               
               
                   
                 3784 
                 largeT-SV40 
                 683-697 
                 HNQPYHICRGFTCFK 
               
               
                   
                 3660 
                 largeT-SV40 
                 129-143 
                 KRKVEDPKDFPSELL 
               
               
                   
                 3637 
                 largeT-SV40 
                  17-31 
                 LGLERSAWGNIPLMR 
               
               
                   
                 3723 
                 largeT-SV40 
                 412-426 
                 MVYNIPKKRYWLFKG 
               
               
                   
                 3653 
                 largeT-SV40 
                  84-98 
                 PTYGTDEWEQWWNAF 
               
               
                   
                 3638 
                 largeT-SV40 
                  21-35 
                 RSAWGNIPLMRKAYL 
               
               
                   
                 3783 
                 largeT-SV40 
                 679-693 
                 SVHDHNQPYHICRGF 
               
               
                   
               
               
                 YP_003708383 
                 4126 
                 smallT-SV40 
                 114-128 
                 LLCLLRMKHENRKLY 
               
               
                   
               
               
                 YP_003708381 
                 1900 
                 VP1-SV40 
                   1-15 
                 MKMAPAKRKGSCPGA 
               
               
                   
               
               
                 YP_003708379 
                 2755 
                 VP2-SV40 
                 123-137 
                 LYRPDDYYDILFPGV 
               
               
                   
               
               
                   
                 2754 
                 VP2-SV40 
                 119-133 
                 MAVDLYRPDDYYDIL 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 19  
               
               
                   
               
               
                 The sequences of the 635 peptides mentioned in  
               
               
                 Table 6 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
            
               
                 0_05 
                 PLSYSRSSEEAFLEA 
                 1_00 
                 LDKDNAYPVECWVPD 
               
               
                   
               
               
                 1_00 
                 APKKPKEPVQVPKLL 
                 1_00 
                 LDKNNAYPVECWIPD 
               
               
                   
               
               
                 1_00 
                 ARFFRLHFRQRRVKN 
                 1_00 
                 LDKNNAYPVECWVPD 
               
               
                   
               
               
                 1_00 
                 AVGGEPLELQGVLAN 
                 1_00 
                 LELQGVLANYRTKYP 
               
               
                   
               
               
                 1_00 
                 AVTVQTEVIGITSML 
                 1_00 
                 LMNYRSKYPDGTITP 
               
               
                   
               
               
                 1_00 
                 DKNKAYPVECWVPDP 
                 1_00 
                 LPATVTLQATGPILN 
               
               
                   
               
               
                 1_00 
                 DMKVWELYRMETELL 
                 1_00 
                 LPGDPDMIRYIDKQG 
               
               
                   
               
               
                 1_00 
                 DMLPCYSVARIPLPN 
                 1_00 
                 LPGDPDMIRYIDRQG 
               
               
                   
               
               
                 1_00 
                 DRKMLPCYSTARIPL 
                 1_00 
                 LPGDPDMMRYVDKYG 
               
               
                   
               
               
                 1_00 
                 EETPDADTTVCYSLA 
                 1_00 
                 LSDLINRRTQRVDGQ 
               
               
                   
               
               
                 1_00 
                 ELLVVPLVNALGNTN 
                 1_00 
                 MESQVEEVRVFDGTE 
               
               
                   
               
               
                 1_00 
                 FFAVGGEPLELQGVL 
                 1_00 
                 MQGVLMNYRSKYPDG 
               
               
                   
               
               
                 1_00 
                 FFRLHFRQRRVKNPF 
                 1_00 
                 MSCTPCRPQKRLTRP 
               
               
                   
               
               
                 1_00 
                 FLNPQMGNPDEHQKG 
                 1_00 
                 NQVSECDMLIWELYR 
               
               
                   
               
               
                 1_00 
                 FLTPEMGDPDEHLRG 
                 1_00 
                 PDMIRYIDKQGQLQT 
               
               
                   
               
               
                 1_00 
                 GGIEVLGVKTGVDSF 
                 1_00 
                 PDMMRYVDKYGQLQT 
               
               
                   
               
               
                 1_00 
                 GGVEVLAAVPLSEET 
                 1_00 
                 PLEMQGVLMNYRTKY 
               
               
                   
               
               
                 1_00 
                 KAYLDKNNAYPVECW 
                 1_00 
                 PYPISFLLSDLINRR 
               
               
                   
               
               
                 1_00 
                 KRKGSCPGAAPKKPK 
                 1_00 
                 QLPRTVTLQSQTPLL 
               
               
                   
               
               
                 1_00 
                 QVAPPDIPNQVSECD 
                 1_01 
                 LMREAVTVKTEVMGI 
               
               
                   
               
               
                 1_00 
                 RMETELLVVPLVNAL 
                 1_01 
                 LPCYSTARIPLPNLY 
               
               
                   
               
               
                 1_00 
                 RYFKIRLRKRSVKNP 
                 1_01 
                 LTCGNLLMWEAVTLQ 
               
               
                   
               
               
                 1_00 
                 SPERKMLPCYSTARI 
                 1_01 
                 MLPCYSAARIPLPNL 
               
               
                   
               
               
                 1_00 
                 TFESDSPNRDMLPCY 
                 1_01 
                 MWEAATVKTEVIGIT 
               
               
                   
               
               
                 1_00 
                 TLHVYNSNTPKAKVT 
                 1_01 
                 MWEAVQVQTEVIGIT 
               
               
                   
               
               
                 1_00 
                 TSGTQQWKGLPRYFK 
                 1_01 
                 NLLMWEAVTVQTEVT 
               
               
                   
               
               
                 1_00 
                 VECFLTPEMGDPDEH 
                 1_01 
                 PLEMQGVLLNYRTKY 
               
               
                   
               
               
                 1_00 
                 VMNTEHKAYLDKNKA 
                 1_01 
                 PLEMQGVLMNYWTKY 
               
               
                   
               
               
                 1_00 
                 VPLVNALGNTNGVVH 
                 1_01 
                 PNLNEDLTCENLLMW 
               
               
                   
               
               
                 1_00 
                 VQSQVMNTEHKAYLD 
                 1_01 
                 PNLNEDLTCGNLLMR 
               
               
                   
               
               
                 1_00 
                 VSECDMKVWELYRME 
                 1_01 
                 PNLNEDLTCGNLLVW 
               
               
                   
               
               
                 1_00 
                 WAPDPSRNDNCRYFG 
                 1_01 
                 PNLNEDLTRGNLLMW 
               
               
                   
               
               
                 1_00 
                 WELYRMETELLVVPL 
                 1_01 
                 PQRKMLPCYSTARIP 
               
               
                   
               
               
                 1_00 
                 WVADPSRNDNCRYFG 
                 1_01 
                 PYPISFSLSDLINRR 
               
               
                   
               
               
                 1_00 
                 YFGRMVGGAATPPVV 
                 1_01 
                 RIPLPNLNEDLTCEN 
               
               
                   
               
               
                 1_00 
                 YFGTLTGGENVPPVL 
                 1_01 
                 SFLLSDLITRRTQRV 
               
               
                   
               
               
                 1_00 
                 YNSNTPKAKVTSERY 
                 1_01 
                 SPERKMLPCYGTARI 
               
               
                   
               
               
                 1_00 
                 YSTARIPLPNLNEDL 
                 1_01 
                 TKYPHGTITPKNPTV 
               
               
                   
               
               
                 1_00 
                 YSVARIPLPNLNEDL 
                 1_01 
                 VSAADICGLFINSSG 
               
               
                   
               
               
                 1_01 
                 CGNLLMREAVTVKTE 
                 1_01 
                 YSAARIPLPNLNEDL 
               
               
                   
               
               
                 1_01 
                 DFSSDSPERKLLPCY 
                 1_01 
                 YSLKLTAENAFDSDS 
               
               
                   
               
               
                 1_01 
                 EHGGGKPIQGSNFHR 
                 1_02 
                 ALELQGVVCNYRTKY 
               
               
                   
               
               
                 1_01 
                 EKKMLPCYSTARIPL 
                 1_02 
                 ALELQGVVFNYGTKY 
               
               
                   
               
               
                 1_01 
                 EMGDSDENLRGFSLK 
                 1_02 
                 ARIPLPILNEDLTCG 
               
               
                   
               
               
                 1_01 
                 ENLRGFSLKLSAEYD 
                 1_02 
                 CGNIPMWEAVTLKTE 
               
               
                   
               
               
                 1_01 
                 EVECFLNPEMGDSDE 
                 1_02 
                 CWVPDPTRNENPRYF 
               
               
                   
               
               
                 1_01 
                 FLNPEMGDSDENLRG 
                 1_02 
                 DEFGVGLLCKGDNLY 
               
               
                   
               
               
                 1_01 
                 IPLPNLYEDLTCGNL 
                 1_02 
                 DKTKAYPVECWVPDP 
               
               
                   
               
               
                 1_01 
                 ITEVECFPNPEMGDP 
                 1_02 
                 DMMRYVDRYGQLQTQ 
               
               
                   
               
               
                 1_01 
                 KLSAKNDFSSDSPDR 
                 1_02 
                 DPDMMRYVDRYGQLQ 
               
               
                   
               
               
                 1_01 
                 KMLPCCSTARIPLPN 
                 1_02 
                 DPDVMRYVDRYGQLQ 
               
               
                   
               
               
                 1_01 
                 KMLPCYGTARIPLPN 
                 1_02 
                 D5IAEVECFLTPEMG 
               
               
                   
               
               
                 1_01 
                 KMLPCYSTTRIPLPN 
                 1_02 
                 DTLPCYSVARIPLPN 
               
               
                   
               
               
                 1_01 
                 KMLPCYSTVRIPLPN 
                 1_02 
                 DVLPCYSVARIPLPN 
               
               
                   
               
               
                 1_01 
                 KPEEPVQVPKLLIKG 
                 1_02 
                 EDLTCGNIPMWEAVT 
               
               
                   
               
               
                 1_01 
                 LARYFKTRLRKRSVK 
                 1_02 
                 EELPEDPDMMRYVDR 
               
               
                   
               
               
                 1_01 
                 LARYFRIRLRKRSVK 
                 1_02 
                 EELPGDPDMIRYVDR 
               
               
                   
               
               
                 1_02 
                 EELPGDPDVMRYVDR 
                 1_02 
                 LLDEFGVGPLCKGVN 
               
               
                   
               
               
                 1_02 
                 EEVRVFEGTEGLPGD 
                 1_02 
                 LLTDLINRRTPKVDG 
               
               
                   
               
               
                 1_02 
                 EHKAYLDRNKAYPVE 
                 1_02 
                 LLTDLINRRTPRIDG 
               
               
                   
               
               
                 1_02 
                 FFLTDLINRRTPRVD 
                 1_02 
                 LPGDPDMMRYVDRYG 
               
               
                   
               
               
                 1_02 
                 FGVGPLCKGANLYLS 
                 1_02 
                 LPILNEDLTCGNILM 
               
               
                   
               
               
                 1_02 
                 FLLADLINRRTPRVD 
                 1_02 
                 MGDPDEHLRGFSKLI 
               
               
                   
               
               
                 1_02 
                 FNYGTKYPDGTIFPK 
                 1_02 
                 MGDPNEHLRGFSKSI 
               
               
                   
               
               
                 1_02 
                 FNYRTKYPDGTIYPK 
                 1_02 
                 MKMAPTKRKGERKDP 
               
               
                   
               
               
                 1_02 
                 FNYRTMYPDGTIFPK 
                 1_02 
                 MMRYVDRYGQLQTKT 
               
               
                   
               
               
                 1_02 
                 FNYRTRYPDGTIFPK 
                 1_02 
                 MMRYVDSCRQKCCNQ 
               
               
                   
               
               
                 1_02 
                 FNYRTTYPDGPIFPK 
                 1_02 
                 MRYVDRYGQLQTQML 
               
               
                   
               
               
                 1_02 
                 FNYRTTYPDGTIFPK 
                 1_02 
                 MRYVDRYGQSQTMML 
               
               
                   
               
               
                 1_02 
                 FNYRTTYPHGTIFPK 
                 1_02 
                 NRSGFQQWRGLSRYF 
               
               
                   
               
               
                 1_02 
                 FPLTDLINRRTPRVD 
                 1_02 
                 NRSGPQQWRGLSRYF 
               
               
                   
               
               
                 1_02 
                 FRYFKVQLRKRRVKN 
                 1_02 
                 NVPPVLHITNTASTV 
               
               
                   
               
               
                 1_02 
                 FTKRSGSQQWRGLSR 
                 1_02 
                 NVPPVLHITNTATTA 
               
               
                   
               
               
                 1_02 
                 GDNLYLSAADVCGMF 
                 1_02 
                 PDMMRYVDRYGQSQT 
               
               
                   
               
               
                 1_02 
                 GDNLYLSAVDVCDMF 
                 1_02 
                 PGDPDMMRYVDRYGQ 
               
               
                   
               
               
                 1_02 
                 GDNLYLSAVDVCGLF 
                 1_02 
                 PNLNEDLTCGNIPMW 
               
               
                   
               
               
                 1_02 
                 GDNLYLSAVDVRGMF 
                 1_02 
                 QPMYGMDAQVKEVRV 
               
               
                   
               
               
                 1_02 
                 GDNLYLSAVDVYGMF 
                 1_02 
                 QSQVMNPEPKGYLDK 
               
               
                   
               
               
                 1_02 
                 GDPDMIRYVDRYGQL 
                 1_02 
                 RKGRVKNPYPISFLL 
               
               
                   
               
               
                 1_02 
                 GDPDMMRYVDRYGQL 
                 1_02 
                 RKRKVKNPYPISFLL 
               
               
                   
               
               
                 1_02 
                 GDPDMMRYVDSCRQK 
                 1_02 
                 RKRRIKNPYPISFLL 
               
               
                   
               
               
                 1_02 
                 GMFTNKSGSQQWRGL 
                 1_02 
                 RKRRVKDPYPISFLL 
               
               
                   
               
               
                 1_02 
                 GMFTNRCGSQQWRGL 
                 1_02 
                 SKDMLPRFSVARIPL 
               
               
                   
               
               
                 1_02 
                 GMFTNRSCSQQWRGL 
                 1_02 
                 SRYFKVELRKRRVKN 
               
               
                   
               
               
                 1_02 
                 IRYVDRYGQLQTKML 
                 1_02 
                 SRYFKVQLRKRKVKN 
               
               
                   
               
               
                 1_02 
                 KNATVQSQVMNTDHK 
                 1_02 
                 SRYFKVQLRKRRVKD 
               
               
                   
               
               
                 1_02 
                 KVELRKRRVKNPYPI 
                 1_02 
                 SRYFKVQPRKRRVKN 
               
               
                   
               
               
                 1_02 
                 KVQLRKRKVKNPYPI 
                 1_02 
                 TEELPGDPDMITYVD 
               
               
                   
               
               
                 1_02 
                 KVQLRKRRVKDPYPI 
                 1_02 
                 TIFPKNATVQSQVVN 
               
               
                   
               
               
                 1_02 
                 LDEFGGGPLCKGDNL 
                 1_02 
                 TTGKLDEFGVGPLCK 
               
               
                   
               
               
                 1_02 
                 LDKNKAYPVECWGPD 
                 1_02 
                 TTVLLDDFGVGPLCK 
               
               
                   
               
               
                 1_02 
                 LDKNKAYPVECWVPN 
                 1_02 
                 TTVLLDEFGAGPLCK 
               
               
                   
               
               
                 1_02 
                 LINIRTPRVDGQPMY 
                 1_02 
                 TTVLLDEFGGGPLCK 
               
               
                   
               
               
                 1_02 
                 LINRRTPGVDGQPMY 
                 1_02 
                 TTVLLDEFGVRPLCK 
               
               
                   
               
               
                 1_02 
                 LINRRTPRVNGQPMY 
                 1_02 
                 TTVLLDELGVGPLCK 
               
               
                   
               
               
                 1_02 
                 TTVLLDEYGVGPLCK 
                 1_04 
                 KASSTCKTPKRQCIP 
               
               
                   
               
               
                 1_02 
                 VARIPLPNINEDLTC 
                 1_04 
                 KRWVKNPYPVVNLIN 
               
               
                   
               
               
                 1_02 
                 VARVPLPNLNEDLTC 
                 1_04 
                 LDENGVGPLCKGDGL 
               
               
                   
               
               
                 1_02 
                 VDSCRQKCCNQKPLL 
                 1_04 
                 LDLQGLVLDYQTQYP 
               
               
                   
               
               
                 1_02 
                 VECFLTPEMGDPDGH 
                 1_04 
                 LRKRWVKNPYPVVNL 
               
               
                   
               
               
                 1_02 
                 VFNYRTKYPDGPIFP 
                 1_04 
                 MFAIGEEPLDLQGLV 
               
               
                   
               
               
                 1_02 
                 VGGEALELQGGAFNY 
                 1_04 
                 MFAIGGEPLDLQGLV 
               
               
                   
               
               
                 1_02 
                 VGGEALELQGVAFNY 
                 1_04 
                 NEDITCDTLQMWEAI 
               
               
                   
               
               
                 1_02 
                 VGGEALELQGVVCNY 
                 1_04 
                 NKDGNYPIEVWCPDP 
               
               
                   
               
               
                 1_02 
                 VGGEALELQGVVFNY 
                 1_04 
                 PGDPDIVRFLDKFGQ 
               
               
                   
               
               
                 1_02 
                 VKNPYPISFPLTDLI 
                 1_04 
                 RVSLPMLNEDITCDT 
               
               
                   
               
               
                 1_02 
                 VMNTEHKAYLDKNKV 
                 1_04 
                 SLINVHYWDMKRVHD 
               
               
                   
               
               
                 1_02 
                 VMNTEHKAYLDRNKA 
                 1_04 
                 SPDLPTTSNWYTYTY 
               
               
                   
               
               
                 1_02 
                 VVNTEHKAYLDKNKA 
                 1_04 
                 TTVLLDENGVGPLCK 
               
               
                   
               
               
                 1_02 
                 WRGLSRYFKVQPRKR 
                 1_04 
                 VGISSLINVHYWVMK 
               
               
                   
               
               
                 1_02 
                 WRGLSRYFRVQLRKR 
                 1_04 
                 VHDYGAGIPVSGVNY 
               
               
                   
               
               
                 1_02 
                 YLDENKAYPVECWVP 
                 1_04 
                 VHYWVMKRVHDYGAG 
               
               
                   
               
               
                 1_02 
                 YLDKNKVYPVECWVP 
                 1_04 
                 YEGSEPLPGDPDIVR 
               
               
                   
               
               
                 1_02 
                 YLDRNKAYPVECWVP 
                 1_05 
                 APKKPKEPVQVPKLV 
               
               
                   
               
               
                 1_02 
                 YLSAVDVCGMFTDRS 
                 1_05 
                 AVVGEPLELQGVLAN 
               
               
                   
               
               
                 1_02 
                 YLYKNKAYPVECWVP 
                 1_05 
                 KMAPAKRKGSCPGAA 
               
               
                   
               
               
                 1_02 
                 YPISFLLADLINRRT 
                 1_05 
                 MKMAPAKRKGSCPGA 
               
               
                   
               
               
                 1_02 
                 YPISFPLTDLINRRT 
                 1_05 
                 MKMAPTKRKGSCPGA 
               
               
                   
               
               
                 1_02 
                 YPITFLLTDLINRRT 
                 1_05 
                 TTVLLDEQGAGPLCK 
               
               
                   
               
               
                 1_03 
                 KVTSERYSVEWAPDP 
                 1_06 
                 GSHMGGVDVLAAVPL 
               
               
                   
               
               
                 1_03 
                 LWLQGRLYITCADML 
                 1_06 
                 KGGVDVLSAVPLSEE 
               
               
                   
               
               
                 1_03 
                 MSCTACRPQKRLTRP 
                 1_06 
                 MACTAKPACTPKPGR 
               
               
                   
               
               
                 1_03 
                 QLPRTVTLQSQAPLL 
                 1_06 
                 NQVSECDMIIWELYR 
               
               
                   
               
               
                 1_03 
                 RMETELLVVPLVNAG 
                 1_06 
                 PDIPNQVSECDMIIW 
               
               
                   
               
               
                 1_03 
                 VVRGAATPPDVSYGN 
                 1_07 
                 AITQIEAYLNPRMGN 
               
               
                   
               
               
                 1_03 
                 YSISSAIHDKESGSI 
                 1_07 
                 ATTPPVMQFTNSVTT 
               
               
                   
               
               
                 1_04 
                 AKLDKDGNYPIEVWC 
                 1_07 
                 DIVGIHTNYSESQNW 
               
               
                   
               
               
                 1_04 
                 CDTLQMWEAISVKTE 
                 1_07 
                 EGLPGDPDLDRYVDK 
               
               
                   
               
               
                 1_04 
                 EPLPGDPDIVRFLDK 
                 1_07 
                 FTGGATTPPVMQFTN 
               
               
                   
               
               
                 1_04 
                 EVRIYEGSEPLPGDP 
                 1_07 
                 IEAYLNPRMGNNNPT 
               
               
                   
               
               
                 1_04 
                 GAGIPVSGVNYHMFA 
                 1_07 
                 KTCPTPAPVPKLLVK 
               
               
                   
               
               
                 1_04 
                 GKAPLKGPQKASQKE 
                 1_07 
                 LNPRMGNNNPTDELY 
               
               
                   
               
               
                 1_04 
                 GKAPLKGPQQASQKE 
                 1_07 
                 MWEAVSVKTEVMGIS 
               
               
                   
               
               
                 1_07 
                 SDNPNATTLPTYSVA 
                 1_09 
                 GNPTLSDAYSQQRSV 
               
               
                   
               
               
                 1_07 
                 SGLMPQIQGQPMEGT 
                 1_09 
                 MFRQFLQPQKPQVQG 
               
               
                   
               
               
                 1_07 
                 VQGTTLHMFSVGGEP 
                 1_09 
                 MLGMVGYAGNPTLSD 
               
               
                   
               
               
                 1_07 
                 VSVKTEVMGISSLVN 
                 1_09 
                 NQSTTPLVDENGVGI 
               
               
                   
               
               
                 1_07 
                 YPTDMVTIKNMKPVN 
                 1_09 
                 PVEKQTSKEPSKGEL 
               
               
                   
               
               
                 1_07 
                 YSESQNWRGLPRYFN 
                 1_09 
                 QKPQVQGTQPNAVQE 
               
               
                   
               
               
                 1_08 
                 ENTRYYGSYTGGQST 
                 1_09 
                 TAVYQSRGAPYTFTD 
               
               
                   
               
               
                 1_08 
                 GEPLELQFLTGNYRT 
                 1_09 
                 TRKQVTAANFPIEIW 
               
               
                   
               
               
                 1_08 
                 GLPRYFNILLRKRTV 
                 1_09 
                 TVDMMFRQFLQPQKP 
               
               
                   
               
               
                 1_08 
                 GTEGLPGDPDMVRYI 
                 1_09 
                 VTAANFPIEIWSADP 
               
               
                   
               
               
                 1_08 
                 GVSSLVNVHMATKRM 
                 1_09 
                 YKVEAILLPNFASGS 
               
               
                   
               
               
                 1_08 
                 HMATKRMYDDKGIGF 
                 1_09 
                 YTLAVVNLPEIPEAL 
               
               
                   
               
               
                 1_08 
                 IELYLNTRMGQNDES 
                 1_10 
                 AKISVAPKKNTDKKE 
               
               
                   
               
               
                 1_08 
                 IGFPVEGMNFHMFAV 
                 1_10 
                 APTSKFLLQNGELIY 
               
               
                   
               
               
                 1_08 
                 KDGDMQYRGLPRYFN 
                 1_10 
                 ATTGNFQSRGLPYPM 
               
               
                   
               
               
                 1_08 
                 KFGQDKTRPPFPARL 
                 1_10 
                 ATTGNFQSRGLPYTM 
               
               
                   
               
               
                 1_08 
                 KQKLTKDGAFPVECW 
                 1_10 
                 DAMCEDTMIVWEAYR 
               
               
                   
               
               
                 1_08 
                 LPGDPDMVRYIDKFG 
                 1_10 
                 EDTMIVWEAYRLETE 
               
               
                   
               
               
                 1_08 
                 LSTQVEEVRVYDGTE 
                 1_10 
                 FFRVHCRQRRIKHPY 
               
               
                   
               
               
                 1_08 
                 LVNVHMATKRMYDDK 
                 1_10 
                 GPLDVIGINPDPERL 
               
               
                   
               
               
                 1_08 
                 PDMVRYIDKFGQDKT 
                 1_10 
                 ISVAPKKNTDNKKEL 
               
               
                   
               
               
                 1_08 
                 PVLQFTNTVTTVLLD 
                 1_10 
                 RKQVNAANFPVELWV 
               
               
                   
               
               
                 1_08 
                 QSTPPVLQFTNTVTT 
                 1_10 
                 WACGGGPLDVIGINP 
               
               
                   
               
               
                 1_08 
                 RTVRNPYPVSSLLNN 
                 2_01 
                 ANQRTAPQWMLPLLL 
               
               
                   
               
               
                 1_08 
                 RYIDKFGQDKTRPPF 
                 2_01 
                 EYYSDLSPIRPSMVR 
               
               
                   
               
               
                 1_08 
                 TEVVGVSSLVNVHMA 
                 2_01 
                 MALELFNPDEYYDIL 
               
               
                   
               
               
                 1_08 
                 TKDGAFPVECWCPDP 
                 2_01 
                 WHVIRDDIPAITSQE 
               
               
                   
               
               
                 1_08 
                 TQGLNPHYKQKLTKD 
                 2_02 
                 AFVNNIHYLDPRHWG 
               
               
                   
               
               
                 1_08 
                 VEGMNFHMFAVGGEP 
                 2_02 
                 ANQRSAPQWMLPLLL 
               
               
                   
               
               
                 1_08 
                 VSVKTEVVGVSSLVN 
                 2_02 
                 APGGANQRSAPQWML 
               
               
                   
               
               
                 1_08 
                 YRTDYSANDKLVVPP 
                 2_02 
                 EDYYDILFPGVNAFV 
               
               
                   
               
               
                 1_08 
                 YYGSYTGGQSTPPVL 
                 2_02 
                 KVSTVGLFQQPAMAL 
               
               
                   
               
               
                 1_08 
                 AAGAANLFGPPVEKQ 
                 2_02 
                 MALQLFNPEDYYDIL 
               
               
                   
               
               
                 1_09 
                 ANLFGPPVEKQTSKE 
                 2_02 
                 NLVRDDLPSLTSQEI 
               
               
                   
               
               
                 1_09 
                 CGGSPLDVIGINPDP 
                 2_02 
                 PGVNAFVNNIHYLDP 
               
               
                   
               
               
                 1_09 
                 EDTIYKVEAILLPNF 
                 2_02 
                 YLDPRHWGPSLFSTI 
               
               
                   
               
               
                 1_09 
                 ETELIFTPQVGSAGY 
                 2_02 
                 YYSRLSPVRPSMVRQ 
               
               
                   
               
               
                 1_09 
                 FLQPQKPQVQGTQPN 
                 2_03 
                 FNALSEGVHRLGQWI 
               
               
                   
               
               
                 2_03 
                 GLAALGGITEGAALL 
                 2_06 
                 RERELLQIAAGQPVD 
               
               
                   
               
               
                 2_03 
                 KRKQDELHPVSPTKK 
                 2_06 
                 RIAYGIWTSYYNTGR 
               
               
                   
               
               
                 2_03 
                 LPELPSLQDVFNRIA 
                 2_06 
                 VVNRAVSEELQRLLG 
               
               
                   
               
               
                 2_03 
                 LVASYLPELPSLQDV 
                 2_06 
                 YNLETGIPGVPDWVF 
               
               
                   
               
               
                 2_03 
                 MALVPIPEYQLETGI 
                 2_12 
                 ASLATVEGITTTSEA 
               
               
                   
               
               
                 2_03 
                 PIPEYQLETGIPGIP 
                 2_12 
                 DDLPSLTSREIQRRT 
               
               
                   
               
               
                 2_03 
                 PSLQDVFNRIAFGIW 
                 2_12 
                 DYYSNLSPIRPSMVR 
               
               
                   
               
               
                 2_03 
                 PVNAIATQVRSLATT 
                 2_12 
                 IAGFAALIQTVTGVS 
               
               
                   
               
               
                 2_03 
                 TGGTPHYATPDWILY 
                 2_12 
                 LLGLYGTVTPALAAY 
               
               
                   
               
               
                 2_03 
                 VHKPIHAPYSGMALV 
                 2_12 
                 NLVRDDLPALTSQEI 
               
               
                   
               
               
                 2_03 
                 VLSDEIQRLLRDLEY 
                 2_12 
                 NLVRDDLPSLTSREI 
               
               
                   
               
               
                 2_03 
                 YQLETGIPGIPDWLF 
                 2_12 
                 QQPVMALQLFNPEDY 
               
               
                   
               
               
                 2_04 
                 HIGGTLQQQTPDWLL 
                 3_04 
                 TVGVRLSREQVSLVN 
               
               
                   
               
               
                 2_04 
                 LDPLQWENSLLHSVG 
                 4_01 
                 AIDQYMVVFEDVKGT 
               
               
                   
               
               
                 2_04 
                 MAFSLDPLQWENSLL 
                 4_01 
                 CLLPKMDSVIFDFLH 
               
               
                   
               
               
                 2_04 
                 QWENSLLHSVGQDIF 
                 4_01 
                 DFATDIQSRIVEWKE 
               
               
                   
               
               
                 2_04 
                 RHALMAFSLDPLQWE 
                 4_01 
                 DIQSRIVEWKERLDS 
               
               
                   
               
               
                 2_04 
                 TLQQQTPDWLLPLVL 
                 4_01 
                 EELHLCKGFQCFKRP 
               
               
                   
               
               
                 2_05 
                 ADSIQQVTERWEAQS 
                 4_01 
                 ELGVAIDQYMVVFED 
               
               
                   
               
               
                 2_05 
                 APQWMLPLLLGLYGS 
                 4_01 
                 ESMELMDLLGLERAA 
               
               
                   
               
               
                 2_05 
                 DDYYDILFPGVQTFV 
                 4_01 
                 EYLLYSALTRDPYHT 
               
               
                   
               
               
                 2_05 
                 KAYEDGPNKKKRKLS 
                 4_01 
                 FFLTPHRHRVSAINN 
               
               
                   
               
               
                 2_05 
                 MAVDLYRPDDYYDIL 
                 4_01 
                 FLHCIVFNVPKRRYW 
               
               
                   
               
               
                 2_05 
                 PGVQTFVHSVQYLDP 
                 4_01 
                 GGDEDKMKRMNTLYK 
               
               
                   
               
               
                 2_05 
                 QDYYSTLSPIRPTMV 
                 4_01 
                 HGINNLDSLRDYLDG 
               
               
                   
               
               
                 2_05 
                 SVQYLDPRHWGPTLF 
                 4_01 
                 ISMYTFSRMKYNICM 
               
               
                   
               
               
                 2_05 
                 TTWTVINAPVNWYNS 
                 4_01 
                 KRVDTLHMTREEMLT 
               
               
                   
               
               
                 2_06 
                 DVFNRIAYGIWTSYY 
                 4_01 
                 KYSVTFISRHMCAGH 
               
               
                   
               
               
                 2_06 
                 ELQRLLGDLEYGFRT 
                 4_01 
                 LCKGFQCFKRPKTPP 
               
               
                   
               
               
                 2_06 
                 FIASHLPELPSLQDV 
                 4_01 
                 LDSEISMYTFSRMKY 
               
               
                   
               
               
                 2_06 
                 GGIYTALAADRPGDL 
                 4_01 
                 LGLERAAWGNLPLMR 
               
               
                   
               
               
                 2_06 
                 GIWTSYYNTGRTVVN 
                 4_01 
                 LMRKAYLRKCKEFHP 
               
               
                   
               
               
                 2_06 
                 GLAALGGLTESAALL 
                 4_01 
                 LNREESMELMDLLGL 
               
               
                   
               
               
                 2_06 
                 LLGDLEYGFRTALAT 
                 4_01 
                 MAGVAWLHCLLPKMD 
               
               
                   
               
               
                 2_06 
                 MALAPIPEYNLETGI 
                 4_01 
                 MDKVLNREESMELMD 
               
               
                   
               
               
                 2_06 
                 PDWILYVLEELNSDI 
                 4_01 
                 RVSAINNFCQKLCTF 
               
               
                   
               
               
                 2_06 
                 PIPEYNLETGIPGVP 
                 4_01 
                 TFSRMKYNICMGKCI 
               
               
                   
               
               
                 2_06 
                 PSLQDVFNRIAYGIW 
                 4_01 
                 VKVNLEKKHLNKRTQ 
               
               
                   
               
               
                 4_02 
                 CGGKSLNVNMPLERL 
                 4_03 
                 LNINIPSEKLPFELG 
               
               
                   
               
               
                 4_02 
                 EHCTYHICKGFQCFK 
                 4_03 
                 LQKYQCSFISKHAFY 
               
               
                   
               
               
                 4_02 
                 FLKCIVLNIPKKRYW 
                 4_03 
                 NKRSQIFPPGIVTMN 
               
               
                   
               
               
                 4_02 
                 GNIPVMRKAYLKKCK 
                 4_03 
                 NNLDNLRDYLDGCVE 
               
               
                   
               
               
                 4_02 
                 HFNHHEKHYYNAQIF 
                 4_03 
                 NPYGLYSRMCRQPFN 
               
               
                   
               
               
                 4_02 
                 ISNLDCLRDYLDGSV 
                 4_03 
                 NVVYWKEVLDNYIGL 
               
               
                   
               
               
                 4_02 
                 KGFQCFKKPKTPPPK 
                 4_03 
                 PGIVTMNEYCIPETV 
               
               
                   
               
               
                 4_02 
                 LLDLCGGKSLNVNMP 
                 4_03 
                 PHKHRVSAINNFCKG 
               
               
                   
               
               
                 4_02 
                 LMDLLGLDRSAWGNI 
                 4_03 
                 QCSFISKHAFYNTVL 
               
               
                   
               
               
                 4_02 
                 MKANVGMGRPILDFP 
                 4_03 
                 TMNEYCIPETVAVRF 
               
               
                   
               
               
                 4_02 
                 RKHQNKRTQVFPPGI 
                 4_03 
                 VHDLNEEEDNIWQSS 
               
               
                   
               
               
                 4_02 
                 RVSAINNYCQKLCTF 
                 4_03 
                 YKKLLQKYQCSFISK 
               
               
                   
               
               
                 4_02 
                 SGHGISNLDCLRDYL 
                 4_03 
                 YMASIAWYTGLNKKI 
               
               
                   
               
               
                 4_02 
                 TKCEDVFLLMGMYLD 
                 4_04 
                 AIELYDKIEKFKVDF 
               
               
                   
               
               
                 4_02 
                 TMNEYSVPRTLQARF 
                 4_04 
                 AIYTTSDKAIELYDK 
               
               
                   
               
               
                 4_02 
                 VDSIHMTREEMLVER 
                 4_04 
                 AVSLEKKHVNKKHQI 
               
               
                   
               
               
                 4_02 
                 VGMGRPILDFPREED 
                 4_04 
                 CKKFKKHLERLRDLD 
               
               
                   
               
               
                 4_02 
                 VNLERKHQNKRTQVF 
                 4_04 
                 CKPPYKLLQENKPLL 
               
               
                   
               
               
                 4_02 
                 VPTYGTDEWESWWNT 
                 4_04 
                 CLIWCLPDTTFKPCL 
               
               
                   
               
               
                 4_02 
                 WESWWNTFNEKWDED 
                 4_04 
                 CLPDTTFKPCLQEEI 
               
               
                   
               
               
                 4_02 
                 WNTFNEKWDEDLFCH 
                 4_04 
                 DLDTIDLLYYMGGVA 
               
               
                   
               
               
                 4_03 
                 ALDQYMVVFEDVKGQ 
                 4_04 
                 EKKLQKIIQLLTENI 
               
               
                   
               
               
                 4_03 
                 ALEFDIDDVYYLLGS 
                 4_04 
                 ENIPKYRNIWFKGPI 
               
               
                   
               
               
                 4_03 
                 CSQATPPKKKHAFDA 
                 4_04 
                 ERLRDLDTIDLLYYM 
               
               
                   
               
               
                 4_03 
                 EDLFGEPKEPSLSWN 
                 4_04 
                 HSQSSSSGYGSFSAS 
               
               
                   
               
               
                 4_03 
                 EFVSHAVFSNKCITC 
                 4_04 
                 KPFPCQKCENRSRLK 
               
               
                   
               
               
                 4_03 
                 EIQSNVVYWKEVLDN 
                 4_04 
                 LAHYLDFAKPFPCQK 
               
               
                   
               
               
                 4_03 
                 ELGVALDQYMVVFED 
                 4_04 
                 LCKLLEIAPNCYGNI 
               
               
                   
               
               
                 4_03 
                 FFLTPHKHRVSAINN 
                 4_04 
                 LEIAPNCYGNIPLMK 
               
               
                   
               
               
                 4_03 
                 FLFCKGVNNPYGLYS 
                 4_04 
                 MDLVLNRKEREALCK 
               
               
                   
               
               
                 4_03 
                 GEPKEPSLSWNQIAN 
                 4_04 
                 MPEMYNNLCKPPYKL 
               
               
                   
               
               
                 4_03 
                 GNGVNNLDNLRDYLD 
                 4_04 
                 NKDLQPGQGINNLDN 
               
               
                   
               
               
                 4_03 
                 HKRVHVQNHENAVLL 
                 4_04 
                 NSSRTDGTWEDLFCD 
               
               
                   
               
               
                 4_03 
                 IPGGLKENEFNPEDL 
                 4_04 
                 PCLQEEIKNWKQILQ 
               
               
                   
               
               
                 4_03 
                 IPTYGTPDWDEWWSQ 
                 4_04 
                 QLLTENIPKYRNIWF 
               
               
                   
               
               
                 4_03 
                 KGVNNPYGLYSRMCR 
                 4_04 
                 SVPRNSSRTDGTWED 
               
               
                   
               
               
                 4_03 
                 LDNYIGLTEFATMQM 
                 4_04 
                 TDGTWEDLFCDESLS 
               
               
                   
               
               
                 4_03 
                 LKENEFNPEDLFGEP 
                 4_04 
                 TPVPTDFPIDLSDYL 
               
               
                   
               
               
                 4_04 
                 TSDKAIELYDKIEKF 
                 4_06 
                 EDLLARRFEKILDKM 
               
               
                   
               
               
                 4_04 
                 VDFKSRHACELGCIL 
                 4_06 
                 EEKMKKLNSLYLKLQ 
               
               
                   
               
               
                 4_04 
                 YKLLQENKPLLNYEF 
                 4_06 
                 EFVSQAVFSNRTLTA 
               
               
                   
               
               
                 4_04 
                 YRSSSFTTPKTPPPF 
                 4_06 
                 EKILDKMDKTIKGEQ 
               
               
                   
               
               
                 4_05 
                 AWLHCLLPKMDSVVY 
                 4_06 
                 ELGVAIDQFTVVFED 
               
               
                   
               
               
                 4_05 
                 CLLPKMDSVVYDFLK 
                 4_06 
                 ESLDKTPELMVKRVL 
               
               
                   
               
               
                 4_05 
                 EDPKDFPSELLSFLS 
                 4_06 
                 FILTPFRHRVSAVNN 
               
               
                   
               
               
                 4_05 
                 EFAQSIQSRIVEWKE 
                 4_06 
                 GEFKDQLNWKALSEF 
               
               
                   
               
               
                 4_05 
                 EKMKKMNTLYKKMED 
                 4_06 
                 GEQDVLLYMAGVAWY 
               
               
                   
               
               
                 4_05 
                 ELGVAIDQFLVVFED 
                 4_06 
                 GLNGKIDELVYRYLK 
               
               
                   
               
               
                 4_05 
                 EYLMYSALTRDPFSV 
                 4_06 
                 GNGMSNLDNLRDYLD 
               
               
                   
               
               
                 4_05 
                 FGGFWDATEIPTYGT 
                 4_06 
                 GNLPLMRTKYLSKCK 
               
               
                   
               
               
                 4_05 
                 FGSTGSADIEEWMAG 
                 4_06 
                 GTPDWDYWWSQFNSY 
               
               
                   
               
               
                 4_05 
                 FLKCMVYNIPKKRYW 
                 4_06 
                 IPTYGTPDWDYWWSQ 
               
               
                   
               
               
                 4_05 
                 GNIPLMRKAYLKKCK 
                 4_06 
                 IVENVPKKRYWVFKG 
               
               
                   
               
               
                 4_05 
                 GQGINNLDNLRDYLD 
                 4_06 
                 KCNFASRHSYYNTAL 
               
               
                   
               
               
                 4_05 
                 HNQPYHICRGFTCFK 
                 4_06 
                 KDNATDASLSFPKEL 
               
               
                   
               
               
                 4_05 
                 KEKAALLYKKIMEKY 
                 4_06 
                 LDKYIGLTEFADMQM 
               
               
                   
               
               
                 4_05 
                 KMNTLYKKMEDGVKY 
                 4_06 
                 LEKKHLNKRSQIFPP 
               
               
                   
               
               
                 4_05 
                 KRKVEDPKDFPSELL 
                 4_06 
                 LGLDMTCWGNLPLMR 
               
               
                   
               
               
                 4_05 
                 LGLERSAWGNIPLMR 
                 4_06 
                 LIWCRPVSDFHPCIQ 
               
               
                   
               
               
                 4_05 
                 LMRKAYLKKCKEFHP 
                 4_06 
                 LKENDFKAEDLYGEF 
               
               
                   
               
               
                 4_05 
                 MDKVLNREESLQLMD 
                 4_06 
                 MTCWGNLPLMRTKYL 
               
               
                   
               
               
                 4_05 
                 MLTNRFNDLLDRMDI 
                 4_06 
                 NAYGLYSRMTRDPFT 
               
               
                   
               
               
                 4_05 
                 MVYNIPKKRYWLFKG 
                 4_06 
                 PFRHRVSAVNNFCKG 
               
               
                   
               
               
                 4_05 
                 PTYGTDEWEQWWNAF 
                 4_06 
                 PGIVTMNEYLVPATL 
               
               
                   
               
               
                 4_05 
                 RSAWGNIPLMRKAYL 
                 4_06 
                 PKKKKDNATDASLSF 
               
               
                   
               
               
                 4_05 
                 RVSAINNYAQKLCTF 
                 4_06 
                 SSSQIPTYGTPDWDY 
               
               
                   
               
               
                 4_05 
                 SFQAPQPSQSSQSVH 
                 4_06 
                 TMNEYLVPATLAPRF 
               
               
                   
               
               
                 4_05 
                 SVHDHNQPYHICRGF 
                 4_06 
                 VIHTTKEKAETLYKK 
               
               
                   
               
               
                 4_05 
                 TREQMLTNRFNDLLD 
                 4_06 
                 VKVNLEKKHLNKRSQ 
               
               
                   
               
               
                 4_05 
                 VKYAHQPDFGGFWDA 
                 4_06 
                 YLVPATLAPRFHKTV 
               
               
                   
               
               
                 4_05 
                 VTEYAMETKCDDVLL 
                 4_12 
                 CGGKSLNVNMPLEKL 
               
               
                   
               
               
                 4_05 
                 WDATEIPTYGTDEWE 
                 4_12 
                 DFAQDIQSRIVEWKE 
               
               
                   
               
               
                 4_05 
                 YHICRGFTCFKKPPT 
                 4_12 
                 DSGHGSSTESQSQCC 
               
               
                   
               
               
                 4_06 
                 AAALLDLCGGKALNI 
                 4_12 
                 EYLLYSALTRDPYYI 
               
               
                   
               
               
                 4_06 
                 AWYLGLNGKIDELVY 
                 4_12 
                 EYLLYSALTREPYHT 
               
               
                   
               
               
                 4_06 
                 CVSTVHQLNEEEDEV 
                 4_12 
                 GSVRVNLERKHQNKR 
               
               
                   
               
               
                 4_12 
                 GVNKEYLLYSALTRE 
                 5_04 
                 SGYNARFCRGPGCML 
               
               
                   
               
               
                 4_12 
                 HMTREEMLVQRFNFL 
                 5_04 
                 WQKTLEETDYCLLHL 
               
               
                   
               
               
                 4_12 
                 KRVDSLHMTREEMLT 
                 5_05 
                 HQPDFGGFWDATEVF 
               
               
                   
               
               
                 4_12 
                 LGLDRSAWGNIPIMR 
                 5_05 
                 LLCLLRMKHENRKLY 
               
               
                   
               
               
                 4_12 
                 MLTDRFNHILDKMDL 
                 5_05 
                 LRMKHENRKLYRKDP 
               
               
                   
               
               
                 4_12 
                 SLHMTREEMLTDRFN 
                 5_05 
                 PGVDAIYCKQWPECA 
               
               
                   
               
               
                 4_12 
                 VDSIHMTREEMLVQR 
                 5_06 
                 CNCFYCFLDKRHKQK 
               
               
                   
               
               
                 4_12 
                 YLRKSLSSSEYLLEK 
                 5_06 
                 ELCCNFPPRKYRLVG 
               
               
                   
               
               
                 5_01 
                 CADFPLCPDTLYCKE 
                 5_06 
                 FYYLCNCFYCFLDKR 
               
               
                   
               
               
                 5_01 
                 LRHLNRKFLRKEPLV 
                 5_06 
                 KIFRKPPMWIECYCY 
               
               
                   
               
               
                 5_01 
                 PDFGTWSSSEVCADF 
                 5_06 
                 KPPMWIECYCYRCYR 
               
               
                   
               
               
                 5_01 
                 SSEVCADFPLCPDTL 
                 5_06 
                 KQKYKIFRKPPMWIE 
               
               
                   
               
               
                 5_02 
                 CDFPPNSDTLYCKEW 
                 5_06 
                 SSSQVECTELCCNFP 
               
               
                   
               
               
                 5_02 
                 FGTWNSSEVGCDFPP 
                 5_06 
                 YCFLDKRHKQKYKIF 
               
               
                   
               
               
                 5_02 
                 IDCYCFDCFRQWFGC 
                 5_06 
                 YCYRCYREWFGFEIS 
               
               
                   
               
               
                 5_02 
                 LMCMLKLRHRNRKFL 
                 5_12 
                 CADFPLCPDTLYCKD 
               
               
                   
               
               
                 5_02 
                 NCATNPSVHCPCLMC 
                 5_12 
                 EKMKKMNTLYKKMEQ 
               
               
                   
               
               
                 5_03 
                 CIHGYNHECQCIHCI 
                 5_12 
                 FGTWNSSEVCADFPL 
               
               
                   
               
               
                 5_03 
                 CYREWFFFPISMQTF 
                 5_12 
                 FGTWNSSEVSCDFPP 
               
               
                   
               
               
                 5_03 
                 FWKVIIFNTEIRAVQ 
                 5_12 
                 GNLSLMRKAYLRKCK 
               
               
                   
               
               
                 5_03 
                 HCILSKYHKEKYKIY 
                 5_12 
                 HCPCLMCMLKLRHKN 
               
               
                   
               
               
                 5_03 
                 KPPVWIECYCYKCYR 
                 5_12 
                 IDCYCFDCFRQWFGL 
               
               
                   
               
               
                 5_03 
                 QSSQVYCKDLCCNKF 
                 5_12 
                 LGLERAAWGNLSLMR 
               
               
                   
               
               
                 5_03 
                 WIECYCYKCYREWFF 
                 5_12 
                 LMCMLKLRHKNRKFL 
               
               
                   
               
               
                 5_03 
                 YCYKCYREWFFFPIS 
                 5_12 
                 PLCPDTLYCKDWPIC 
               
               
                   
               
               
                 5_03 
                 YIMKQWDVCIHGYNH 
                 5_12 
                 RAAWGNLSLMRKAYL 
               
               
                   
               
               
                 5_03 
                 YYEAYIMKQWDVCIH 
                 6_01 
                 LLEFCRGEDSVDGKN 
               
               
                   
               
               
                 5_04 
                 DYMQSGYNARFCRGP 
                 6_01 
                 QASVKVSKTWTGTKK 
               
               
                   
               
               
                 5_04 
                 FGFPPTWESFDWWQK 
                 6_05 
                 KVRRSWTESKKTAQR 
               
               
                   
               
               
                 5_04 
                 FSMFDEVSTKFPWEE 
                 6_12 
                 LLEFCRGKDSVDGKN 
               
               
                   
               
               
                 5_04 
                 GTLKDYMQSGYNARF 
                 6_12 
                 MVLRQLSRQASVKIG 
               
               
                   
               
               
                 5_04 
                 KQKNCLTWGECFCYQ 
                 6_12 
                 QASVKIGKTWTGTKK 
               
               
                   
               
               
                 5_04 
                 RGPGCMLKQLRDSKC 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 20 
               
             
            
               
                   
               
               
                 Gene ID for polyomavirus peptides 
               
            
           
           
               
               
            
               
                   
                 Gene ID for polyomaviruses 
               
            
           
           
               
               
               
               
               
               
               
               
               
            
               
                   
                 BKV 
                 JCV 
                 KIV 
                 MCV 
                 SV40 
                 WUV 
                 HPyV6 
                 HPyV7 
               
               
                   
               
               
                 VP1 
                 _1_01 
                 _1_02 
                 _1_03 
                 _1_04 
                 _1_05 
                 _1_06 
                 _1_09 
                 _1_10 
               
               
                 VP2 
                 _2_01 
                 _2_02 
                 _2_03 
                 _2_04 
                 _2_05 
                 _2_06 
                 _2_09 
                 _2_10 
               
               
                 large T 
                 _4_01 
                 _4_02 
                 _4_03 
                 _4_04 
                 _4_05 
                 _4_06 
                 _4_09 
                 _4_10 
               
               
                 small T 
                 _5_01 
                 _5_02 
                 _5_03 
                 _5_04 
                 _5_05 
                 _5_06 
                 _5_09 
                 _5_10