Patent Publication Number: US-11638738-B2

Title: Process to enhance the bioactivity of Ashwagandha extracts

Description:
This Application is a continuation of U.S. application Ser. No. 16/265,440 filed Feb. 1, 2019, which is a divisional of U.S. application Ser. No. 15/326,450 filed Jan. 13, 2017, which is a 371 of International Application Ser. No. PCT/IN2016/050354 filed Oct. 18, 2016, which claims priority to Indian Provisional Appl. Ser. No. 5691/CHE/2015 filed Oct. 22, 2015, which are all incorporated in entirety by reference. 
    
    
     FIELD 
     Methods to enhance the bioactivity of Ashwagandha extract is provided. Compositions of Ashwagandha extract enriched with withanolide glycosides and saponins after removing alkaloids, withanolide aglycones and oligosaccharides are disclosed. Processes for manufacturing extracts of Ashwagandha enriched with withanolide glycosides, saponins and for removal of alkaloids, withanolide aglycones and oligosaccharides are provided. Some embodiments provide a process of extraction of Ashwagandha and enrichment mainly with withanolide glycosides to obtain a percentage of withanolide glycosides in a range of 0.5% and above and saponins in a range of 0.1 and above. The withanolide glycosides include withanoside I to VII and sitoindosides I to X. Methods of extraction of Ashwagandha extract enriched in withanolide glycosides and saponins but having alkaloids below 0.1%, withanolide aglycones below 0.1% and oligosaccharides below 0.1% are provided. 
     Some embodiments provide an enteric coated Ashwagandha extract composition to protect the withanolide glycosides from hydrolysis under acidic condition, preventing conversion of withanolide glycosides to aglycones and delivery of withanolide glycoside in neutral/alkaline pH in gastrointestinal tract (GIT). Some embodiments provide an enteric coated Ashwagandha extract composition to increase the bioactivity of withanolide glycosides by preventing conversion of withanolide glycosides to withanolide aglycones in acidic environment. 
     Enteric coated Ashwagandha extracts showed antistress activity, immunomodulatory activity, antidiabetic activity and anti-inflammatory activity even at a lower dosage level compared to extracts of Ashwagandha not coated with enteric coating. Methods of increasing the bioavailability and bioactivity of withanolide glycosides after administering enteric coated Ashwagandha extract composition at a lower dosage level than extracts of Ashwagandha that are not coated with enteric coating are provided. Suitable dosage forms of enteric coated Ashwagandha extract composition are provided. 
     Method of increasing the endurance after administering enteric coated Ashwagandha extract in combination with Amaranth extract at a lower dosage level is provided. 
     BACKGROUND 
       Withania somnifera  a popular Indian medicinal plant also known as Ashwagandha, Indian ginseng, and winter cherry belongs to the Solanaceae or nightshade family. It has been an important herb in the ayurvedic and indigenous medical system for over 3000 years. 
     It is an erect, greyish, subshrub with inconspicuous yellow or greenish flowers followed by small, spherical, orangish-red berries containing yellow, kidney-shaped seeds. It grows three-to-five feet tall, mainly on waste land, but is cultivated widely as the whole plant; most commonly the root and leaf are used medicinally. The fruits from several of its species are edible, and some are used in traditional medicine. 
     The species name  somnifera  means “sleep-inducing” in Latin. The common name comes from the Sanskrit ashwagandha, i.e ashwa for horse, and gandha for smell, hence the common idea that the name means “smells like a horse.” The species is widely distributed in the northwestern Indian states of Gujarat, Madhya Pradesh, Maharashtra, Rajasthan, Uttar Pradesh, and the Punjab plains extending to the mountainous regions of Himachal Pradesh and Jammu. It also is cultivated in parts of Madhya Pradesh and Rajasthan. Northwest of India, its habitat extends into the Pakistani provinces of Sindh and Baluchistan, and on into Afghanistan. To the southeast of India, it occurs in Sri Lanka. In China, it is reported to grow in the western provinces of Gansu and Yunnan. 
     Ashwagandha is one of the rasayana herbs in Ayurveda—one of the herbs that purportedly promotes youth and longevity and alleviates suffering. It is thought to be especially rejuvenative for men; to strengthen bone marrow, muscle, and semen; and to imbue the user with intellectual facility, in addition to long life and youthful vitality. However, it also is believed to be quite helpful to the elderly by providing energy and relieving pain, inflammation, and nervous debility. 
     The roots of this plant have been used as an adaptogen and to treat arthritis, asthma, dyspepsia, hypertension, rheumatism, and syphilis. Earlier pharmacological investigations of  Withania somnifera  have revealed its antiinflammatory, antioxidant, immunomodulatory, and tumor cell proliferation inhibitory activities. 
     The leaves are used to expel worms and are combined with warm castor oil ( Ricinis communis , Euphorbiaceae) on carbuncles, inflammations, and swellings. The Masai use the leaf juice for conjunctivitis. The fresh bruised berries are used on ringworm. The fruits or seeds are used to coagulate milk. The seeds also are used as a masticatory. A bark infusion is used in Lesotho internally for asthma and externally for bedsores. The tender shoots are eaten as a vegetable in India. 
     The dried root and the whole plant are used in the traditional medicine systems of Ayurveda, Siddha, Sowa-Rigpa (Amchi), and Unani, as well as in Indian folk medicine. The materials of commerce are obtained from both cultivated and wild-collected sources, mainly in India. 
     In countries where the Ayurvedic system of medicine is officially recognized and practiced (e.g., India, Bangladesh, Bhutan, Malaysia, Nepal, and Sri Lanka), the powdered dried root of Ashwagandha is used, as a component of preparations, for treating inflammatory disorders, phthisis (any wasting or atrophic disease, weakness, diseases due to vata dosha), and male impotence. 
     In countries where the Unani system of medicine is officially recognized and practiced (e.g., Bangladesh, India, Malaysia, Pakistan, and Sri Lanka), the dried mature root, referred to as “asgand,” is used as a component of medicinal formulations to treat leucorrhoea, spermatorrhoea, decreased viscosity of semen, sexual debility, lumbago, and arthritis. 
     In Siddha medicine—a Dravidian system of medicine originating in the southeastern Indian state of Tamil Nadu, now also practiced in the neighboring states of Karnataka, Kerala, and Andhra Pradesh, as well as in parts of Malaysia, Singapore, and Sri Lanka—the dried root (purified before use), referred to as amukkara, is used as a component of formulations indicated for treatment of conditions including oligospermia, lancinating pain, loss of body strength, anemia, convulsions/seizures/fits, disordered humor, eczema, edema/swelling, and tuberculosis. 
     Dhuley et al found that the root extract of Ashwagandha prevented the rise of experimentally induced LPO in rabbits &amp; mice. Withaferin A and Sitoindoside VIII-X exhibits fairly potent anti-arthritic, anti-inflammatory, antioxidant &amp; immuno modulant activities, they also increase in the levels of SOD, CAT, GPX in brain &amp; the steroidal lactone W.A (Bhattacharya et al, 1997). The administration of Ashwagandha Rasayana significantly reduced the lung tumor nodule formation and also reduced leucopenia induced by cyclophosphamide treated experimental animals, indicating its usefulness in cancer therapy (Menon L, 1997 and Davis L, 1998).  Withania  increase the WBC count, reduce leucopenia. They also increased bone marrow cellularity &amp; normalised the ratio of hormachromatic erythrocytes &amp; polychromatic erythrocytes (Davis L, 1998). A methanolic &amp; 80% ethanolic extract of  Withania somnifera  displayed significant anti-inflammatory activity on carrageenan-induced paw edema (Hindawi-al MKIH et al, 1989). 
     The main constituents of Aswagandha are alkaloids and steroidal lactones. Among the various alkaloids, withanine is the main constituent. The other alkaloids are somniferine, somnine, somniferinine, withananine, pseudowithanine, tropine, pseudotropine, 3agloyloxytropane, choline, cuscohygrine, isopelletierine, anaferine and anahydrine. Two acyl steryl glucoside viz. sitoindoside VII and sitoindoside VIII have been isolated from root. The leaves contain steroidal lactones, which are commonly called withanolides. The withanolides have C28 steroidal nucleus with C9 side chain, having six membered lactone rings. Withaferin A a steroidal lactone is the most important withanolide isolated from the extract of the leaves and dried roots of  Withania somnifera.    
     Qamar et al in 2012 reported the active phytochemical and pharmacological components of  Withania somnifera . The roots are reported to contain alkaloids, amino acids, steroids, volatile oil, starch, reducing sugars, glycosides, hentriacontane, dulcitol, withaniol, an acid and a neutral compound. Many biochemically heterogeneous alkaloids have been reported in the roots. Basic alkaloids include cuscohygrine, anahygrine, tropine, pseudotropine, anaferine, isopelletierine, withananine, withananinine, pseudo-withanine, somnine, somniferine, somniferinine. Neutral alkaloids include 3-tropyltigloate and an unidentified alkaloid. Other alkaloids include withanine, withasomnine, and visamine. The leaves of the plant (Indian chemotype) are reported to contain 12 withanolides, 5 unidentified alkaloids, many free amino acids, chlorogenic acid, glycosides, glucose, condensed tannins, and flavonoids (Khare, 2007). The stem of the plant contains condensed tannins and flavonoids. The bark contains a number of free amino acids (Anonymous, 1982). 
     Patel et al. (2009) extracted powdered root of  W. somnifera  with ethylacetate and developed fingerprint profile and analysis of withaferin A in young and old root samples. Chatterjee et al. (2010) studied the metabolic fingerprinting of  W. somnifera  leaf and root extracts by using serial exhaustive extraction using hexane, 90% methanol, 70% methanol, chloroform and butanol. Rao et al. (2012) used methanol to extract withanolides from the roots of the  W. somnifera  and the extract was subjected to HPLC for detection. Singh et al. (2011) performed TLC to identify the different constituents present in different extracts of aswagandha roots. Different solvent systems such as acetonitrile:water (75:25) and toluene:ethyl acetate:acetic acid (65:33:2). HPTLC method has been developed by Sharma et al. (2007) for the estimation of withaferin-A and withanolide-A in different plant parts such as leaf, root, stem and fruit of two morphotypes of  W. somnifera.    
     Ghosal et al, US patent publication 2004/0166184, reveals composition of  Withania somnifera  from roots and leaves containing 8-25% withanolide glycosides and sitoindosides, about 25-75% Oligosaccharides and less than 2% of free withanferin A (aglycone). Ghosal et al U.S. Pat. No. 6,153,198 discloses a high purity  Withania Somnifera  extract composition from root of Ashwagandha containing at least 3% withanolide glycoside and sitoindoside, at least 3% oligosaccharides and less than 0.5% of cytotoxic withferin A (aglycones) in the form of a high purity stable powder for producing an enhanced cognition effect for the use and to augment the learning facility. U.S. Pat. No. 7,108,870, Sangwan et al, reported an improved process of analytical and quantitative isolation of withaferin A from  Withania somnifera . Patent publication US 20140087009, McNeary et al, discloses a composition including combinations of β-glucan and  Withania Somnifera  for increasing the immune activity of certain target cytokines ad phagocytosis and reducing cortisol or corticosterone. Sumithradevi et al reported a simple method to purify withanolide A from the roots of  Withania somnifera . Uddin et al reported the phytochemical and pharmacological profile of  Withania somnifera . WO2012160569, Jayesh Panalal et al discloses a process for extraction of Withanoside IV and V from Ashwagandha roots and its composition. 
     SUMMARY 
     A method to enhance the bioactivity of extracts of Ashwagandha is provided. Disclosure provides a stable composition obtained from the extract of Ashwagandha. Disclosure provides an extract of Ashwagandha enriched with withanolide glycosides and saponins from which alkaloids, withanolide aglycones and oligosaccharides are decreased or removed. Some embodiments of the extract of Ashwagandha contain withanolide glycoside in a range of about 0.5% and above, saponins in a range of 0.1 and above, alkaloids less than about 0.1%, withanolide aglycones less than about 0.1% and oligosaccharides less than about 0.1%. 
     The disclosure provides an enteric coated Ashwagandha extract in suitable dosage forms to obtain a stable composition. The disclosure provides methods for producing the Ashwagandha extract. The disclosure provides methods for producing the Ashwagandha extract enriched with withanolide glycosides content, saponin content, after removal of alkaloids, withanolide aglycones and oligosaccharides. The extract of Ashwagandha can be prepared from root, stems, leaves and whole plant of Ashwagandha. Disclosure provides a method of increasing the bioactivity of withanolide glycosides after administering enteric coated Ashwagandha extract composition even at a lower dosage level. Disclosure further provides an enteric coated Ashwagandha extract composition is found to have antistress activity, immunomodulatory activity, antidiabetic activity, anti-inflammatory activity etc even at a lower dosage level. 
     The disclosure provides an enteric coated Ashwagandha extract in combination with Amaranth extract for enhancing the endurance. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG.  1    provides method of preparation of dichloromethane extract of ammonia treated Ashwagandha root. 
         FIG.  2    provides method of preparation of 60% methanol extract of ammonia treated Ashwagandha root. 
         FIG.  3    provides method of preparation of 50% methanol extract of Ashwagandha root. 
         FIG.  4    provides method of preparation of purified 50% methanol extract of ammonia treated Ashwagandha root. 
         FIG.  5    and  FIG.  5   (CONT.) provide method of preparation of column purified 50% methanol elute of ammonia treated Ashwagandha root. 
         FIG.  6    provides method of preparation of chloroform extract of 60% methanol extract of Ashwagandha root. 
         FIG.  7    provides a method of preparation of 20% ethanol extract of ammonia treated Ashwagandha root. 
         FIG.  8    provides a method of preparation of purified water extract of Amaranth. 
         FIG.  9    provides a method of preparation of combination of enteric coated Ashwagandha extract and purified Amaranth extract. 
     
    
    
     DETAILED DESCRIPTION 
     Though Ashwagandha extracts are known to have beneficial properties, we found during our studies that the alkaloids and fraction enriched with alkaloid components was having toxic effects in animals. The non alkaloid fraction was not showing toxicity but retained the beneficial effects of Ashwagandha and was found to be superior than the whole extract. The compounds identified from the Ashwagandha root extract which had toxicity included alkaloid compounds, withaferin A, withanine, withanolide aglycones. We removed toxic components from Ashwagandha through a novel extraction and purification process. At the same time percentage of active components like withanolide glycosides and saponins in the extract was enriched through the novel extraction and purification process. 
     Activity of different Ashwagandha extracts or powders in different pH was studied. Withanolide glycosides undergo hydrolysis in acidic pH and gets converted to withanolide aglycones. The withanolide aglycones have toxic effects. This conversion will happen in the acidic environment of the stomach after oral intake of regular Ashwagandha extract. An enteric coating can will prevent the contact of withanolide glycosides with acidic pH in stomach and release the contents in areas with neutral or mildly alkaline pH in the gastro intestinal tract from where it is absorbed. 
     An extract of Ashwagandha with enteric coating will release the actives (withanolide glycosides) in the small intestine without releasing in stomach thereby preventing the hydrolysis to aglycones and enhancing the absorption and oral bioavailability of withanolide glycoside in the body. 
     Methods to enhance the bioactivity of Ashwagandha extract are provided. A highly stable composition of Ashwagandha extract enriched with withanolide glycosides and saponins after removal of alkaloids, withanolide aglycones and oligosaccharides is provided. The disclosure also provides processes for manufacturing enriched extracts of Ashwagandha after the removal of alkaloids and oligosaccharides. Disclosure also relates enteric coated Ashwagandha extracts. The extracts are enriched with withanolide glycosides and saponins after the removal of alkaloids, withanolide aglycones and oligosaccharides. 
     An enteric coated Ashwagandha extract composition is provided. An enteric coating protects the withanolide glycosides from hydrolysis under acidic condition, preventing conversion of withanolide glycosides to aglycones and delivery of withanolide glycoside in neutral/alkaline pH in gastrointestinal tract (GIT). Some embodiments provide an enteric coated Ashwagandha extract composition to increase the bioavailability of withanolide glycosides at lower dosage level by preventing conversion of withanolide glycosides to withanolide aglycones in acidic environment. Enteric coated Ashwagandha extract composition is found to have antistress activity, immunomodulatory activity, antidiabetic activity, anti-inflammatory activity, to improve sleep quality etc. Methods of increasing the bioavailability and bioactivity of withanolide glycosides after administering enteric coated Ashwagandha extract composition are provided. Method of enhancing the endurance after administering enteric coated Ashwagandha extract in combination with Amaranth extract is provided. 
     The disclosure provides a method to enhance the bioactivity of Ashwagandha extract. Disclosure provides a stable Ashwagandha composition prepared by a unique method of extraction. The disclosed processes provide an enteric coated Ashwagandha extract enriched with withanolide glycosides include withanoside I to VII and sitoindoside I to X after removal of alkaloid, withanolide aglycones and oligosaccharides. 
     The disclosure relates to a composition of Ashwagandha root extract. The composition enriched with withanolide glycosides content (withanoside I to VII, sitoindosides I to X) after removal of alkaloid, withanolide aglycones and oligosaccharides. 
     Ashwagandha root extract is analysed by HPLC and found to contain withanolide glycosides (withanoside I to VII), sitoindosides (sitoindosides I to X) and the presence is confirmed by LCMS analysis. Ashwagandha extract contain saponins and it is analysed by UV method. 
     The disclosure provides Ashwagandha powder, Ashwagandha powder and/or extract and formulation. The disclosure also provides enteric coated powder or granules of extract of Ashwagandha powder. The enteric coated Ashwagandha extract was found to have antistress activity, immunomodulatory activity, antidiabetic activity, anti-inflammatory activity, to improve sleep quality etc. Ashwagandha extract without coating was also found to have anti stress activity, immunomodulatory activity, antidiabetic activity, anti-inflammatory activity, to improve sleep quality etc. 
     In some embodiments, Ashwagandha powder and/or extract is blended with a second extract. The second extract is selected from the group consisting of amla extract, turmeric extract, grape seed extract, green tea extract, pomegranate extract, Amaranth extract, costus extract, cocoa extract, coconut root extract, rosemary extract, mint leaf extract, star anise, sweet basil extract, cinnamon extract/clove extract, ginger extract, cumin seed extract, black pepper extract, fenugreek extract, or combinations thereof. 
     In another embodiment Ashwagandha powder and/or extract is blended with a second extract and the combination is coated with an enteric coating material. The enteric coated Ashwagandha powder and/or extract blended with a second extract is found to have antistress activity, immunomodulatory activity, antidiabetic activity, anti-inflammatory activity, to improve sleep quality etc. 
     In some embodiments, Ashwagandha extract can be fortified by adding Amaranth extract. Some embodiments provide combination of Ashwagandha extract and Amaranth extract for enhancing the endurance. Some embodiments provide combination of Ashwagandha extract and Amaranth extract for enhancing the endurance wherein the ratio of Ashwagandha extract to Amaranth extract is 1:1. In some embodiments, the weight ratio of Ashwagandha extract to Amaranth extract is 3:1. In another embodiment, the ratio of Ashwagandha extract to Amaranth extract is 1:3. 
     Some embodiments provides an enteric coated extract of Ashwagandha combined with Amaranth extract in different ratios. In some embodiments, the weight ratio of coated Ashwagandha extract to Amaranth extract is 3:1. In some embodiments the weight ratio of coated Ashwagandha extract to Amaranth extract is 1:1. In another embodiment, the ratio of coated Ashwagandha extract to Amaranth extract is 1:3. 
     The disclosure provides methods for producing the Amaranth extract. The extract of Amaranth can be prepared from fresh or dried leaves and stem of Amaranth. 
     In some embodiments of the extract of Amaranth, the Amaranth is selected from the group consisting of  Amaranthus caudatus, Amaranthus cruentus, Amaranthus tricolor, Amaranthus blitum, Amaranthus viridis, Amaranthus dubis  or combinations thereof. 
     In some embodiments of the extract of Amaranth, nitrates ranges from about 0.1% to about 3%. In some embodiments of the extract, nitrates ranges from about 1% to about 10%. In some embodiments of the extract, nitrates ranges from about 10% to about 20%. In some embodiments of the extract, nitrates ranges from about 3% to about 20%. In some embodiments of the extract, nitrates ranges from about 0.1% to about 80%. 
     In some embodiments the nitrate rich vegetables include juice, extract, powder and the like of Amaranth, Cabbage, Spinach, Beetroot, Artichoke, Asparagus, Broad Bean, Eggplant, holy basil, gymnema sylvestre, Garlic, Onion, gingko, Green Bean, Green tea, hawthorn berry, kelp, Mushroom, Pea, Pepper, Potato, Summer Squash, Sweet Potato, salvia, Tomato, tribulus, Watermelon, Broccoli, Carrot, Cauliflower, Cucumber, Pumpkin, Chicory, Dill, Turnip, Savoy Cabbage, Celeriac, Chinese Cabbage, Endive, Fennel, Kohlrabi, Leek, Parsley, Celery, Cress, Chervil, Lettuce, Rocket (Rucola), and the like. 
     In another embodiment, the nitrite salt is selected from the group consisting of sodium nitrite, potassium nitrite, magnesium nitrite, calcium nitrite, and mixtures thereof. In a particular embodiment, the nitrite salt is selected from the group consisting of sodium nitrite, potassium nitrite, and mixtures thereof. 
     The Ashwagandha extract or powder can be obtained from Ashwagandha whole plant or fresh root or dried root, stem or leaves of Ashwagandha or their combinations. 
     A regular extract of Ashwagandha contain a composition of withaferin A, withanolides, alkaloids, aminoacids, steroids, glycosides, condensed tannins, flavonoids and oligosaccharides. The components alkaloids and withaferin A (withanolides or withanolide aglycones) are found to be toxic. So we purified the extract by fractionation to remove the toxic components. This unique extraction process results an extract of Ashwagandha enriched with active components like withanolide glycosides and saponins, wherein withanolide glycoside include withanoside I-VII and sitoindosides I to X. 
     We found during our studies that the alkaloids and fraction enriched with alkaloid components was having toxic effects in animals. Animals fed with alkaloidal rich fraction has decrease in spontaneous movements, sluggish response to stimuli and diminished muscle tone. Whereas there is no toxic symptoms in animals fed with alkaloidal free purified Ashwagandha root extract with 80% withanolide glycosides, enteric coated alkaloidal free purified Ashwagandha root extract with 80% withanolide glycosides. 
     Animal fed with Ashwagandha root extract with minimum 5% withanolide glycosides or alkaloidal rich fraction shows loss of appetite whereas the animals fed with alkaloids free purified Ashwagandha root extract with 80% withanolide glycosides and enteric coated alkaloids free purified Ashwagandha root extract with 80% withanolide glycosides shows normal appetite. In our study we can observe that some animals fed with Ashwagandha root extract with minimum 5% withanolide glycosides and alkaloidal rich fraction died due to clonic convulsions and respiratory depression. 
     Some embodiments provide a composition obtained from the extract of Ashwagandha from roots of Ashwagandha. The extract has enriched withanolide glycoside and saponin content after removal of alkaloid content, withaferin A, withanolide aglycones wherein withanolide glycosides are present in the extract in a range of 30% and above, saponins are present in the range of 10% and above, alkaloids are present in the range of about 0.001% to about 0.1%, withanolide aglycones are present in the range of about 0.001% to about 0.1% and oligosaccharides are present in the range of less than 0.1%. 
     In some embodiments of the extract, withanolide glycosides range about 5% and above, saponins range about 2% and above, alkaloids are less than about 0.1%, withanolide aglycones range less than about 0.1%, and oligosaccharides are less than 0.1%. In some embodiments of extracts prepared from root and stem of Ashwagandha, withanolide glycosides are about 6%, oligosaccharides about 5% and withanolide aglycones about 0.2%, and a weight ratio of withanolide glycoside to aglycones is about 30:1, and, a weight ratio of withanolide glycoside to oligosaccharides is about 6:5. 
     In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides ranges from about 0.5% to about 80%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides range from about 0.5% to about 3.5%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides range from about 0.5% to about 5%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides range from about 3.5% to about 5%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides range from about 0.5% to about 35%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides range from about 3.5% to about 35%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides range from about 5% to about 35%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides ranges from about 30% to about 40%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides ranges from about 30% to about 50%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides ranges from about 30% to about 80%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides are at least about 0.5%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides are at least about 3.5%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides are at least about 5%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides are at least about 35%. In some embodiments of the Ashwagandha extract or enteric coated Ashwagandha compositions, withanolide glycosides are at least about 80%. In some embodiments, saponins ranges from about 0.1% to 2%. In some embodiments, saponins ranges from about 0.1% to 10%. In some embodiments, saponins ranges from about 2% to 30%. In some embodiments, alkaloids ranges from about 0.001% to about 0.05%. In some embodiments, alkaloids ranges from about 0.001% to about 0.1%. In some embodiments, withanolide aglycones ranges from about 0.001% to about 0.05%. In some embodiments, withanolide aglycones ranges from about 0.001% to about 0.1%. In some embodiments, oligosaccharides ranges from about 0.001% to about 0.05%. In some embodiments, oligosaccharides ranges from about 0.001% to about 0.1%. 
     Some embodiments provide a composition comprising an extract of root of  Withania somnifera . The extract of root of  Withania somnifera  has at least about 35% withanolide glycosides and 10% saponins. The extract also has about 0.4% alkaloids. Withanolide aglycones and oligosaccharides were undetectable in the extract by HPLC method. The withanolide glycosides included sitoindoside I to X, withanoside I to VII and withanmides. 
     Some embodiments provide a composition of an extract of root of  Withania somnifera . The extract has at least about 80% withanolide glycosides. The extract has about 15% saponins. The extract has about 0.001% alkaloids. Withanolide aglycones and oligosaccharides were undetectable in the extract by HPLC method. The withanolide glycosides included sitoindoside I to X, withanoside I to VII and withanmides. 
     Some embodiments provide an enteric coated composition of an extract of  Withania somnifera . The extract of  Withania somnifera  includes withanolide glycosides. The enteric coated composition has an enteric coating material. The enteric coating material can be poly (methacrylic acid-co-methyl methacrylate), esters of aleurtic acid, cellulose acetate phthalate, cellulose acetate trimellitate, poly (vinyl acetate phthalate), hydroxypropyl methyl cellulose, hydroxypropyl methylcellulose phthalate, hydroxy propyl methyl cellulose acetate succinate, acetaldehyde dimethyl cellulose acetate, chitosan, zein, fatty acids, waxes, shellac, plastics, plant fibers, or, a combination of modified ethyl cellulose and sodium alginate. The extract of  Withania somnifera  in the enteric coated composition can include at least about 0.5% withanolide glycosides. In some embodiments of the enteric coated composition, the extract of  Withania somnifera  has at least about 3.5% withanolide glycosides. In some embodiments of the enteric coated composition, the extract of  Withania somnifera  has at least about 35% withanolide glycosides. In some embodiments of the enteric coated composition, the extract of  Withania somnifera  has at least about 80% withanolide glycosides. In some embodiments, the extract of  Withania somnifera  in the enteric coated composition has at least about 0.1% saponins; about 0.1% alkaloids; about 0.1% withanolide aglycones; and, about 0.1% oligosaccharides. 
     In some embodiments, the extract of  Withania somnifera  in the enteric coated composition has about 2.5% saponins; about 0.06% alkaloids; about 0.15% withanolide aglycones, and, about 3% oligosaccharides. In some embodiments, the extract of  Withania somnifera  in the enteric coated composition has about 10% saponins; about 0.4% alkaloids; and, withanolide aglycones and oligosaccharides were undetectable by HPLC method. In some embodiments, the extract of  Withania somnifera  in the enteric coated composition has about 15% saponins; about 0.001% alkaloids. In some embodiments, withanolide aglycones and oligosaccharides were undetectable by HPLC method. In some embodiments, the withanolide glycosides included sitoindoside I to X, withanoside I to VII and withanmides. 
     Some embodiments provide a composition having a combination of an extract of Amaranth and an enteric coated extract of  Withania somnifera . Some embodiments of the combination of Amaranth extract and enteric coated extract of  Withania somnifera  provide improved endurance. 
     Some embodiments provide a method of preparing an extract of  Withania somnifera . The method includes cleaning roots of  Withania somnifera , followed by treating cleaned fresh roots of  Withania somnifera  with ammonia. Then extracting the cleaned fresh roots with methylene dichloride to obtain a filtrate and a residue. The residue is washed with water to obtain a wash having a pH ranging from about 6 to about 7. The washed residue is dried at about 90° C. to about 100° C. to obtain a dried residue. The dried residue is powdered to obtain an extract of roots of  Withania somnifera . The extract of roots of  Withania somnifera  is a powder. In some embodiments the method further includes, preparing granules of the powder followed by spraying the granules with an enteric coating material to obtain an enteric coated composition. The extract of root of  Withania somnifera  has at least about 3% withanolide glycosides and at least about 1% saponins. Some embodiments provide an enteric coated extract of roots of  Withania somnifera , wherein the extract of root of  Withania somnifera  has at least about 3% withanolide glycosides and at least about 1% saponins. 
     Some embodiments provide a method of preparing an extract of  Withania somnifera . The method includes cleaning roots of  Withania somnifera , followed by treating cleaned fresh roots of  Withania somnifera  with ammonia. Then extracting the cleaned fresh roots with methylene dichloride to obtain a filterate and a residue. The residue is washed with water to obtain a wash having a pH ranging from about 6 to about 7. The washed residue is dried at about 90° C. to about 100° C. to obtain a dried residue. The dried residue is powdered to obtain an extract of roots of  Withania somnifera . The extract of roots of  Withania somnifera  is a powder. Some embodiments of the method further include extracting the powder with methanol at about 60° C. to about 70° C. to obtain a supernatant and a residue. Then concentrating the supernatant to obtain a concentrate. Next, drying the concentrate to obtain dried methanol extract in the form of a powder. In some embodiments the method further includes, preparing granules of the powder followed by spraying the granules with an enteric coating material to obtain an enteric coated composition. The extract of root of  Withania somnifera  has at least about 5% withanolide glycosides and at least about 1.5% saponins. Some embodiments provide an enteric coated extract of roots of  Withania somnifera , wherein the extract of root of  Withania somnifera  has at least about 5% withanolide glycosides and at least about 1.5% saponins. 
     Some embodiments provide a method of preparing an extract of  Withania somnifera . The method includes cleaning roots of  Withania somnifera , followed by treating cleaned fresh roots of  Withania somnifera  with ammonia. Then extracting the cleaned fresh roots with methylene dichloride to obtain a filterate and a residue. The residue is washed with water to obtain a wash having a pH ranging from about 6 to about 7. The washed residue is dried at about 90° C. to about 100° C. to obtain a dried residue. The dried residue is powdered to obtain an extract of roots of  Withania somnifera . The extract of roots of  Withania somnifera  is a powder. Some embodiments of the method further include extracting the powder with methanol at about 60° C. to about 70° C. to obtain a supernatant and a residue. Then concentrating the supernatant to obtain a concentrate. Next, drying the concentrate to obtain dried methanol extract in the form of a powder. The method further includes dissolving the dried methanol extract in water and clarifying the resulting product to obtain a supernatant and a residue. Then loading the supernatant onto an adsorbing column. Next, eluting the adsorbing column with water and then with 50% methanol. Next, concentrating and drying the 50% methanol elute to obtain a dried methanol extract of roots of  Withania somnifera . The dried methanol extract is a powder. The adsorbing column can be silica, SP700, HP20, HP2MGL, SA10A, WA10, CRB03, CRB05, CR20, XAD 7HP, FP66, SK1B, or SP825L. In some embodiments the method further includes, preparing granules of the powder followed by spraying the granules with an enteric coating material to obtain an enteric coated composition. The extract of root of  Withania somnifera  has at least about 35% withanolide glycosides and at least about 10% saponins. Some embodiments provide an enteric coated extract of roots of  Withania somnifera , wherein the extract of root of  Withania somnifera  has at least about 35% withanolide glycosides and at least about 10% saponins. 
     Some embodiments provide a method of preparing an extract of  Withania somnifera . The method includes cleaning roots of  Withania somnifera , followed by treating cleaned fresh roots of  Withania somnifera  with ammonia. Then extracting the cleaned fresh roots with methylene dichloride to obtain a filterate and a residue. The residue is washed with water to obtain a wash having a pH ranging from about 6 to about 7. The washed residue is dried at about 90° C. to about 100° C. to obtain a dried residue. The dried residue is powdered to obtain an extract of roots of  Withania somnifera . The extract of roots of  Withania somnifera  is a powder. Some embodiments of the method further include extracting the powder with methanol at about 60° C. to about 70° C. to obtain a supernatant and a residue. Then concentrating the supernatant to obtain a concentrate. Next, drying the concentrate to obtain dried methanol extract in the form of a powder. The method further includes dissolving the dried methanol extract in water and clarifying the resulting product to obtain a supernatant and a residue. Then loading the supernatant onto an adsorbing column. Next, eluting the adsorbing column with water and then with 50% methanol. Next, concentrating and drying the 50% methanol elute to obtain a dried methanol extract of roots of  Withania somnifera . The dried methanol extract is a powder. The adsorbing column can be silica, SP700, HP20, HP2MGL, SA10A, WA10, CRB03, CRB05, CR20, XAD 7HP, FP66, SK1B, or SP825L. The method further includes dissolving the powder of the dried methanol extract in water, followed by centrifuging to obtain a supernatant and a residue. Next, loading the supernatant onto an adsorbing column. The adsorbing column of can be silica, SP700, HP20, HP2MGL, SA10A, WA10, CRB03, CRB05, CR20, XAD 7HP, FP66, SK1B, and SP825L. Next the adsorbing column is eluted with 10% methanol followed by eluting with 50% methanol to obtain a 50% methanol eluate. The 50% methanol eluate is dissolved in methanol, followed by concentrating the dissolved product. Next, acetone is added to the concentrated product to obtain a precipitate. The precipitate is filtered and dried to obtain a powder of the extract of roots of  Withania somnifera . In some embodiments the method further includes, preparing granules of the powder followed by spraying the granules with an enteric coating material to obtain an enteric coated composition. The extract of root of  Withania somnifera  has at least about 80% withanolide glycosides and at least about 15% saponins. Some embodiments provide an enteric coated extract of roots of  Withania somnifera , wherein the extract of root of  Withania somnifera  has at least about 80% withanolide glycosides and at least about 15% saponins. 
     Some embodiments provide a method of preparing an extract of  Withania somnifera . The method includes cleaning roots of  Withania somnifera , followed by treating cleaned fresh roots of  Withania somnifera  with ammonia. Then extracting the cleaned fresh roots with methylene dichloride to obtain a filterate and a residue. The residue is washed with water to obtain a wash having a pH ranging from about 6 to about 7. The washed residue is dried at about 90° C. to about 100° C. to obtain a dried residue. The dried residue is powdered to obtain an extract of roots of  Withania somnifera . The extract of roots of  Withania somnifera  is a powder. The method further includes, extracting the powder with 20% ethanol at about 75° C. to about 80° C. to obtain a supernatant and a residue. Then concentrating the supernatant to obtain a concentrated ethanol extract. Followed by drying the concentrated ethanol extract to obtain a powder of dried ethanol extract of roots of  Withania somnifera . In some embodiments the method further includes, preparing granules of the powder followed by spraying the granules with an enteric coating material to obtain an enteric coated composition. The extract of root of  Withania somnifera  has at least about 3.5% withanolide glycosides and at least about 2.5% saponins. Some embodiments provide an enteric coated extract of roots of  Withania somnifera , wherein the extract of root of  Withania somnifera  has at least about 3.5% withanolide glycosides and at least about 2.5% saponins. 
     Some embodiments provide a method of enhancing bioactivity of an extract of  Withania somnifera  by administering an enteric coated composition of the extract of  Withania somnifera . Some embodiments provide a method of enhancing bioavailability of withanolide glycosides from an extract of  Withania somnifera  by administering an enteric coated composition of the extract of  Withania somnifera . Some embodiments provide a method of delivering an extract of  Withania somnifera  into a medium having a pH ranging from about 6 to about 9 by administering an enteric coated composition of the extract of  Withania somnifera . The extract of  Withania somnifera  is released from the enteric coated composition of the extract of  Withania somnifera  when the pH of the medium ranges from about 6 to about 9. Some embodiments provide a method of treatment by administering an extract of  Withania somnifera . The conditions treated include improving immunomodulatory activity, improving anti inflammatory activity, improving anti stress activity, and treating diabetes. Immunomodulating activity includes an increase in bone marrow cells, an increase in the number of α-esterase positive cells, an increase in IgG and IgM antibody titre value, enhancement of the number of antibody producing cells. The improvement in anti stress activity is observed as enhanced anoxia stress tolerance time and enhanced swimming endurance time. Some embodiments provide a method of treatment by administering an enteric coated extract of  Withania somnifera . The treatment method conditions include improving immunomodulatory activity, improving anti inflammatory activity, improving anti stress activity and treating diabetes. Improvement in immunomodulating activity includes an increase in bone marrow cells, an increase in the number of α-esterase positive cells, an increase in IgG and IgM antibody titre value, enhancement of the number of antibody producing cells. The improvement in anti stress activity includes enhanced anoxia stress tolerance time and enhanced swimming endurance time. Some embodiments provide a dosage form of the extract of  Withania somnifera  including capsule, tablet, mini tablet, granule, sachet, powder, paste, infusion, ampoule, solution, suspension, emulsion, pills or cream. Some embodiments include a dosage form of enteric coated extract of  Withania somnifera . The dosage form of the enteric coated composition includes capsule, tablet, mini tablet, granule, sachet, powder, paste, infusion, ampoule, solution, suspension, emulsion, pills or cream. A dosage of the extract of  Withania somnifera  in the dosage form of compositions from about 200 mg to about 2000 mg. A dosage of the enteric coated extract of  Withania somnifera  in the dosage form of compositions from about 200 mg to about 2000 mg. 
     Activity of different Ashwagandha extract/powder in different pH was studied. Oral administration of Ashwagandha root powder (200 mg/kg) at pH 1, 2, 3 and 5 after reserpine injection was not much effective and immobility time was recorded as 245, 245, 230 and 220 seconds respectively. Administration of Ashwagandha root powders (200 mg/kg) at pH 7 and 7.4 after reserpine injection was slightly effective and immobility time was 205 seconds. Similarly, administration of Ashwagandha extract with 3.5% withanolide glycosides (60 mg/kg) at pH 1, 2, 3 and 5 after reserpine injection was slightly effective and immobility time was 235, 230, 210 and 189 seconds respectively. Whereas, administration of Ashwagandha extract with 3.5% withanolide glycosides (60 mg/kg) at pH 7 and 7.4 after reserpine injection was more effective and immobility time was recorded as 152 and 150 seconds respectively. Administration of Ashwagandha extract with 35% withanolide glycosides (20 mg/kg) at pH 1, 2, 3 and 5 after reserpine injection was effective and immobility time were 220, 220, 202 and 180 seconds respectively. Whereas, administration of Ashwagandha extract with 35% withanolide glycosides (20 mg/kg) at pH 7 and 7.4 after reserpine injection was more effective and immobility time were about 145 and 139 seconds respectively. In case of administration of extracts containing very high (80%) withanolide glycosides (20 mg/kg) at pH 1, 2, 3 and 5 after reserpine injection is effective and immobility time was about 212, 211, 194 and 165 seconds respectively. Whereas, administration of Ashwagandha extract with 80% withanolide glycosides (20 mg/kg) at pH 7 and 7.4 after reserpine injection was most effective and immobility time was about 110 and 105 seconds respectively. In case of fluoxetine standard at 10 mg/kg (Group XVII), the immobility time was only 122 seconds which was almost similar to normal control animals. The activity of Ashwagandha extract subjected to acidic buffer showed lesser activity compared to extract in subjected to neutral or basic buffer. The extract in neutral or basic pH shows higher activity. 
     From the above study we found that in acidic pH active component of Ashwagandha (Withanolide glycosides) undergo hydrolysis and get converted to withanolide aglycones. The withanolide aglycones have toxic effects. In order to protect the Ashwagandha extract in acidic medium different types of coating is applied to Ashwagandha extract and its activity is studied. Oral administration of Ashwagandha root powder with normal HPC coating (200 mg/kg) after reserpine injection was not much effective and immobility time was 245 seconds. Administration of Ashwagandha root powder with enteric coating (200 mg/kg) after reserpine injection was more effective than HPC coating and immobility time was recorded as 205 seconds. Administration of Ashwagandha root powder with delayed release coating (200 mg/kg) after reserpine injection was less effective than enteric coating and immobility time was recorded as 220 seconds. 
     Similarly, oral administration of Ashwagandha granules with 3.5% withanolide glycosides with normal HPC coating (60 mg/kg) after reserpine injection was not much effective and immobility time was recorded as 215 seconds. Administration of Ashwagandha granules with 3.5% withanolide glycosides with enteric coating (60 mg/kg) after reserpine injection was more effective than HPC coating and immobility time was recorded as 141 seconds. Administration of Ashwagandha granules with 3.5% withanolide glycosides with delayed release coating (60 mg/kg) after reserpine injection was less effective than enteric coating and immobility time was recorded as 186 seconds. 
     Oral administration of Ashwagandha granules with 35% withanolide glycosides with normal HPC coating (20 mg/kg) after reserpine injection is slightly effective and immobility time is recorded as 192 seconds (Group IX). Administration of Ashwagandha granules with 35% withanolide glycosides with enteric coating (20 mg/kg) after reserpine injection was more effective than HPC coating and immobility time is recorded as 138 seconds. Administration of Ashwagandha granules with 35% withanolide glycosides with delayed release coating (20 mg/kg) after reserpine injection is less effective than enteric coating and immobility time is recorded as 162 seconds. 
     Oral administration of Ashwagandha granules with 80% withanolide glycosides with normal HPC coating (20 mg/kg) after reserpine injection is effective and immobility time was recorded as 173 seconds. Administration of Ashwagandha granules with 80% withanolide glycosides with enteric coating (20 mg/kg) after reserpine injection is most effective than HPC coating and immobility time is recorded as 109 seconds. Administration of Ashwagandha granules with 80% withanolide glycosides with delayed release coating (20 mg/kg) after reserpine injection is less effective than enteric coating and immobility time is recorded as 132 seconds. In case of fluoxetine standard at 10 mg/kg, the immobility time is 120 seconds which is similar to normal control animals. 
     From the above study of Ashwagandha extract with different types of coating it is clear that enteric coated extract is found to be more stable in acidic medium. So in our next study we found the activity of Ashwagandha extract with different percentages of enteric coating (0.5% to 15%). Oral administration of Ashwagandha root powder with 0.5%, 1% and 3% enteric coating (200 mg/kg) after reserpine injection is not much effective and immobility time is recorded as 245, 235 and 223 seconds respectively. Administration of Ashwagandha root powder with 5 and 7% enteric coating (200 mg/kg) after reserpine injection is more effective than lower percentage of coatings and immobility time is recorded as 216 and 207 seconds respectively. Administration of Ashwagandha root powder with 10, 12 and 15% enteric coating (200 mg/kg) after reserpine injection is most effective and immobility time is recorded as 201, 200 and 200 seconds respectively. In case of fluoxetine standard at 10 mg/kg, the immobility time was 120 seconds which is similar to normal control animals. 
     Oral administration of Ashwagandha extract with 3.5% withanolide glycosides with 0.5%, 1% and 3% enteric coating (60 mg/kg) after reserpine injection is not much effective and immobility time is recorded as 225, 220 and 206 seconds respectively. Administration of Ashwagandha extract with 3.5% withanolide glycosides with 5 and 7% enteric coating (60 mg/kg) after reserpine injection is more effective than lower percentage of coatings and immobility time is recorded as 195 and 180 seconds respectively. Administration of Ashwagandha extract with 3.5% withanolide glycosides with 10, 12 and 15% enteric coating (60 mg/kg) after reserpine injection is most effective and immobility time is recorded as 160, 142 and 140 seconds respectively. In case of fluoxetine standard at 10 mg/kg, the immobility time is 120 seconds which is similar to normal control animals. 
     Oral administration of Ashwagandha extract with 35% withanolide glycosides with 0.5%, 1% and 3% enteric coating (20 mg/kg) after reserpine injection is not much effective and immobility time is recorded as 200, 188 and 180 seconds respectively. Administration of Ashwagandha extract with 35% withanolide glycosides with 5 and 7% enteric coating (20 mg/kg) after reserpine injection is more effective than lower percentage of coatings and immobility time is recorded as 171 and 160 seconds respectively. Administration of Ashwagandha extract with 35% withanolide glycosides with 10, 12 and 15% enteric coating (20 mg/kg) after reserpine injection is most effective and immobility time is recorded as 153, 139 and 136 seconds respectively. In case of fluoxetine standard at 10 mg/kg, the immobility time is 120 seconds which is similar to normal control animals. 
     Oral administration of Ashwagandha extract with 80% withanolide glycosides with 0.5%, 1% and 3% enteric coating (20 mg/kg) after reserpine injection is not much effective and immobility time is recorded as 195, 183 and 173 seconds respectively. Administration of Ashwagandha extract with 80% withanolide glycosides with 5 and 7% enteric coating (20 mg/kg) after reserpine injection is more effective than lower percentage of coatings and immobility time is recorded as 160 and 145 seconds respectively. Administration of Ashwagandha extract with 80% withanolide glycosides with 10, 12 and 15% enteric coating (20 mg/kg) after reserpine injection is most effective and immobility time is recorded as 122, 111 and 109 seconds respectively. In case of fluoxetine standard at 10 mg/kg, the immobility time was 120 seconds which is similar to normal control animals. 
     Some embodiments provide a composition obtained from the extract of Ashwagandha, encapsulating the composition by a polymeric enteric coating. Enteric coating protects a capsule contents in the highly acidic environment of the stomach. “Enteric” indicates small intestine; therefore enteric coatings prevent release of medication before it reaches the small intestine. Most enteric coatings work by presenting a coated surface that is stable at the highly acidic pH found in the stomach, but breaks down rapidly at a less acidic (relatively more basic) pH. The polymeric enteric coating material include Poly (methacrylic acid-co-methyl methacrylate) also known as Eudragit, Shellac (esters of aleurtic acid), CAP (Cellulose acetate phthalate), CAT (Cellulose acetate trimellitate), PVAP (Poly(vinyl acetate phthalate)), HPMCP (Hydroxypropyl methylcellulose phthalate), Hydroxy propyl methyl cellulose acetate succinate, Acetaldehyde dimethyl cellulose acetate, Chitosan, Zein, fatty acids, waxes, plastics, plant fibers and Modified ethyl cellulose and sodium alginate combination (Nutrateric) and their combinations in any possible ratios. 
     Some embodiments provide a composition obtained from the extract of Ashwagandha, encapsulating the composition by a simple coating (Film coating). The film coating material include Hydroxy propyl methyl cellulose, Hydroxy propyl cellulose, Methyl cellulose, Pectin, Starch, Hydrolyzed starch, Poly vinyl acetate (PVA), Poly vinyl pyrollidone (PVP), Poly vinyl alcohol, Cellulose acetate, Ethyl cellulose, Poly ethylene glycols (PEG), Glyceryl behenate, Hypromellose, Sodium alginate, Sodium propionate, Methacrylate amino ester copolymers, Methyl hydroxyl ethyl cellulose, Sodium carboxy methyl cellulose, Hydroxy ethyl cellulose, Sodium acetate, Sodium metaborate, Maltodextrin, Propylene glycoalginate, Polyvinylpyrrolidone-vinyl acetate copolymer, Polycaprolactones, Acrylic acid copolymer and their combinations in any possible ratios. 
     In one embodiment Ashwagandha root extract and purified Ashwagandha root extract is coated with enteric or nonenteric coating material. 
     Ashwagandha root extract or purified Ashwagandha root extract is loaded into the bowl of the fluid bed extractor (FBE). Hot, filtered air up to 90° C. is passed at high velocity from the bottom of the FBE bowl through the feed material (Ashwagandha root extract or purified root extract) and feed material is fluidised. 
     Meantime, any enteric or non enteric coating material is dissolved in suitable solvent. Coating solution is sprayed into fluidised material by using a spraying devise attached to the FBE. Through the process of fluid bed coating, fluidized particles are continuously sprayed with coating solution, depositing layers (films) of material to the surface of the particles, and yielding an even layer thickness. 
     The activity study shows that after administration of Ashwagandha root extract with minimum 6% withanolide glycosides at 100 mg/Kg dose and 20 mg/Kg dose, Ashwagandha root extract with minimum 6% withanolide glycosides coated with pectin after reserpine injection was not much effective and immobility time was recorded as above 240 seconds. Ashwagandha extract with 6% withanolide glycosides coated with Eudragit after reserpine injection, the immobility time was recorded as 195 seconds. When animals treated with Ashwagandha root extract containing minimum 1% withanolide glycosides, it is not effective and immobility time is found as 242 seconds. When animals treated with Ashwagandha root extract containing minimum 20% withanolide glycosides at 100 and 20 mg/kg dose the immobility time was 235 and 239 seconds respectively. But Ashwagandha root extract with 20% withanolide glycosides coated with Eudragit at 20 mg/kg dose, immobility time was reduced to 190 seconds. The immobility time is found as 175 and 210 seconds when animals treated with purified Ashwagandha extract with 35% withanolide glycosides at 100 and 20 mg/kg dose. When animals treated with purified Ashwagandha extract with 35% withanolide glycosides coated with pectin, HPMC and PVA at 20 mg/kg dose, the immobility time is 205, 207 and 204 seconds respectively which indicates very less effectiveness of such non-enteric coatings. In spite of nonenteric coatings, when animals treated with Ashwagandha extract with 35% withanolide glycosides coated with enteric materials shellac, CAP or Eudragit at 20 mg/kg dose, the immobility time is reduced to 162, 164 and 160 seconds respectively indicating the effectiveness of enteric coating. Animals treated with purified Ashwagandha extract with 80% withanolide glycoside at 100 and 20 mg/kg dose showed immobility time as 166 and 192 seconds respectively. When animals are treated with purified Ashwagandha extract with 80% withanolide glycoside coated with pectin, the immobility time is 189 seconds whereas when same extract is coated with Eudragit, the immobility time is reduced to 122 seconds only which shows the effectiveness of enteric coating. 
     An extract of Ashwagandha without enteric coating will release the actives (withanolide glycosides and sitoindosides) in the stomach. In the acidic environment of stomach withanolide glycosides will convert to withanolide aglycones, which is toxic. 
     But an extract of Ashwagandha with enteric coating will release the actives (withanolide glycosides and sitoindosides) in the small intestine without releasing the actives in the stomach. Enteric coatings in the extract prevent release of actives before it reaches the small intestine. 
     The conversion of withanolide glycosides into aglycon moiety and sugar is studied in simulated gastric fluid. It is found that at 15 minutes in acidic medium withanolide glycosides in tablet without any coating containing purified Ashwagandha root extract is converted into withanolide aglycones (33.8%), but only 1.2 percent of withanolide aglycones are formed following the administration of enteric coated tablet of alkaloid free purified Ashwagandha root extract. At 2 hours 74.8 percent of withanolide aglycones are formed in the acidic environment following the administration of tablet without any coating containing Ashwagandha root extract. After administration of enteric coated tablet of alkaloid free purified Ashwagandha root extract only 4.8 percent of withanolide aglycones are formed in the acidic condition. 
     An enteric coated Ashwagandha extract composition increases the bioavailability of withanolide glycosides. After administration of Ashwagandha extract with 6% withanolide glycosides or Ashwagandha root extract with 6% withanolide glycosides coated with pectin withanolide glycosides are not detected in the plasma. Animals fed with Ashwagandha root extract with 6% withanolide glycosides coated with Eudragit at 20 mg/kg, the withanolide glycoside level was found as 15.2 ng/ml. Withanolide glycosides are not detected in the plasma of animals fed with Ashwagandha extract with 3.5% withanolide glycoside at 100 and 20 mg/kg dose, whereas Ashwagandha root extract with 3.5% withanolide glycosides coated with Eudragit at 20 mg/kg, the withanolide glycoside level is found as 10.2 ng/ml. Animals fed with Ashwagandha extract with minimum 20% withanolide glycosides at 100 and 20 mg/Kg showed withnolide glycoside level in plasma as 1.9 and 0.3 ng/ml respectively. Animals fed with Ashwagandha extract with minimum 1%, and 20% withanolide glycoside coated with Eudragit at 20 mg/kg showed withnolide glycoside level in plasma as 1.6 and 4.2 ng/ml showing the effectiveness of enteric coating in preserving the withanolide glycosides from hydrolysis in the stomach. Animals fed with purified Ashwagandha extract with 35% withanolide glycoside at 100 and 20 mg/kg dose, the withanolide glycoside level in plasma is found as 60.3 and 12.4 ng/ml respectively. Animals fed with purified Ashwagandha extract with 35% withanolide glycoside coated with pectin, HPMC or PVA at 20 mg/kg dose showed plasma level of withanolide glycosides as 13.8, 12.9 and 13.2 ng/ml respectively. When the same extract, i.e. purified Ashwagandha extract with 35% withanolide glycoside is coated with enteric materials shellac, CAP or Eudragit, the plasma level of withanolide glycosides is increased to 100.3, 101.9 and 102.3 ng/ml respectively, indicating the effectiveness of enteric coating. When animals fed with purified Ashwagandha extract with 80% withanolide glycoside at 100 and 20 mg/kg dose, the plasma withanolide glycoside level is found as 85.2 and 18.5 ng/ml respectively. When the same extract (purified Ashwagandha extract with 80% withanolide glycoside) is coated with pectin and fed at only 20 mg/kg dose, the withanolide glycoside level is found as 17.3 ng/ml only showing the noneffectiveness of nonenteric coating. Whereas when animals fed with purified Ashwagandha extract with 80% withanolide glycoside coated with Eudragit at 20 mg/kg dose, the plasma withanolide glycoside level is found as 149.8 ng/ml showing the protective nature of enteric coating. 
     In one embodiment the extract of Ashwagandha can be administered as a minitablet form. In another embodiment the minitablets are prepared by mixing the Ashwagandha extract with binder and lubricant. The mixture is fed in tableting machine having 3 mm dies and punches to get the 3 mm diameter mini tablets with 9 Kg/cm′ hardness. The binder and lubricant used is microcrystalline cellulose and Magnesium stearate. 
     In one embodiment mini tablets are coated by a coating material of 5-20%. In another embodiment minitablets are filled into a capsule for easy administration. 
     In some embodiments, the adsorbing material used for purifying Ashwagandha root extract are silica, SP700, HP20, HP2MGL, SA10A, WA10, CRB03, CRB05, CR20, XAD 7HP, FP66, SK1B, SP825L. 
     The disclosure provides different extracts of Ashwagandha extracted by using solvents like water, methanol, ethanol, chloroform, methylenedichloride, ethylene dichloride, isopropanol, n-butanol, methyl acetate, ethyl acetate, propyl acetate, n-butyl acetate, acetone, and combinations thereof. 
     Low molecular weight alcohols that can be used in preparation of the extract include methanol, ethanol, isopropanol, n-butanol and combinations thereof. Esters that can be used for extract preparation include methyl acetate, ethyl acetate, propyl acetate, n-butyl acetate and combinations thereof. Alkanes that can be used for preparation of the extract include pentane, hexane, heptane, isooctane, and combinations thereof. 
     In some embodiments, a dosage form of the Ashwagandha extract composition for oral administration in suitable dosage forms like capsule, tablet, mini tablet, granule, sachet, powder, paste, infusion, injection, ampoule, solution, suspension, emulsion, pills, cream etc is provided. 
     The disclosure provides an enteric coated Ashwagandha extract/Ashwagandha powder composition in suitable dosage forms like capsule, tablet, mini tablet, granule, sachet, powder, paste, infusion, ampoule, pills, cream etc. 
     These delivery systems may require excipients selected from the group consisting of a disintegrant, diluents, binders, fillers, a carrier, adsorbents, emulsifiers, lubricants, stabilizing agents, antiadherents, galidants, antioxidants and mixtures thereof. 
     Further a dosage form of an Ashwagandha extract is disclosed for administering in a dosage ranging from about 200 mg to about 2000 mg to a human subject. 
     The disclosure provides an enteric coated Ashwagandha extract enriched with withanolide glycosides, saponins and wherein alkaloids, withanolide aglycones and oligosaccharides were removed from the extract. The enteric coated Ashwagandha extract has antistress activity, immunomodulatory activity, antidiabetic, anti-inflammatory activity etc. The enteric coated Ashwagandha extract composition is used to improve sleep quality, maximal aerobic capacity (VO2 max), respiratory exchange ratio (RER), rate of perceived exertion (RPE), heart rate (HR), average absolute and relative power, total time to reach maximum exhaustion, rate pressure product (RPP), respiratory exchange ratio (pCO2/pO2), exercise Intensity, performance and endurance, cardiac output. 
     The disclosure also provides an Ashwagandha raw powder with enteric coating. An enteric coated Ashwagandha raw powder shows antistress activity, immunomodulatory activity, antidiabetic, anti-inflammatory activity, used to improve sleep quality etc. 
     Inflammation is a complex biological response of vascular tissues and immune cells to harmful stimuli, such as pathogens, damaged cells, or irritants. It is characterized by five signs: redness, increased heat, swelling, pain, and/or loss of function. Inflammation can be classified as either acute or chronic. Acute inflammation is the initial response of the body to harmful stimuli and is achieved by the increased movement of plasma and leukocytes (especially granulocytes) from the blood into the injured tissues. A cascade of biochemical events propagates and matures the inflammatory response, involving the local vascular system, the immune system, and various cells within the injured tissue. Prolonged inflammation, known as chronic inflammation, leads to a progressive shift in the type of cells present at the site of inflammation and is characterized by simultaneous destruction and healing of the tissue from the inflammatory process. At present, the majority of medicines widely used as anti-inflammatory agents are nonsteroid anti-inflammatory drugs (NSAIDs) that have, as the mechanism of action, an inhibitory action on cyclooxygenases (COXs) that is involved in the biosynthesis of prostanoids. However, since prostanoid synthesis activity is present in various tissues in the living body and governs the homeostasis thereof, various side effects are induced when NSAID is administered. One test for inflammation is the C-reactive protein (CRP) measurement. Some clinicians are advocating including it routinely. The CRP test detects any inflammation, no matter where it is. A skinned knee, flu, arthritis and infections are common causes of elevated CRP. Anti-inflammatory activity of extracts/drugs can be evaluated in small animals like rats and mice. A classic model is carrageenan induced paw oedema model in rats. Carrageenan is an irritant and produces inflammation in rats paw after injecting a small volume into sub-plantar region. The volume of paw increases within 30 minutes of injecting carrageenan which can be measured by a plethysmometer. Volume of paw should be measured at various time points after carrageenan and test drug administration and should be compared with control. 
     An anti-inflammatory activity of enteric coated Ashwagandha root extract enriched with withanolide glycosides and saponins, wherein the alkaloids, withanolide aglycones and oligosaccharides were removed from the extract, showed higher percentage inhibition of inflammation. A higher value of percentage inhibition indicates more anti-inflammatory activity. Ashwagandha root extract with 3.5% withanolide glycoside coated with Eudragit is also found to be effective in reducing the inflammation in rat paw. 
     Stress is simply a reaction to a stimulus that disturbs the physical or mental equilibrium. It is considered to be any condition which results in perturbation of the body&#39;s homeostasis. Within seconds of an acutely stressful event, norepinephrine is released from nerve endings in preparation for a rapid response, and the adrenal glands release epinephrine and norepinephrine into the bloodstream, resulting in the familiar fight or flight response. Within minutes of a stressful event (and possibly lasting for several hours), a much more complex interaction between the nervous and endocrine systems and other forms of internal communication occurs, resulting in an intricate stress adaptation response. During this time the adrenal glands release extra cortisol into the circulation. 
     Several other endocrine glands are critical to the stress response. The hypothalamus, the “master gland” in the brain, responds to stress by releasing corticotropin-releasing factor (CRF). This hormone signals the pituitary gland to release adrenocorticotropic hormone (ACTH), which stimulates the adrenal glands to release cortisol. With the rise in stress hormones, a complex mechanism of feedback controls is set in motion, eventually signaling the hypothalamus to stop producing CRF. A wide range of events or conditions is considered physiologically stressful because the adrenals are stimulated to release stress hormones. These occurrences include calorie restriction, surgery, sleep deprivation, excessive exercise, and various mental states—all of which can result in elevated cortisol and catecholamine stress hormones. 
     Stress exerts a disruptive influence on normal circadian release of cortisol. A study conducted on military cadets subjected to a five-day training course of heavy physical exercise and food and sleep deprivation found cortisol levels went up and performance deteriorated due to the stressful nature of the training. The researchers also found, “the circadian rhythm was extinguished.” Even after 4-5 days of rest, circadian rhythms had not completely normalized. This and other research demonstrates the physiological and psychological consequences of acute and chronic stress can persist well past cessation of a stressful event. 
     Stress is a factor in many illnesses—from headaches to heart disease, and immune deficiencies to digestive problems. A substantial contributor to stress-induced decline in health appears to be an increased production of stress hormones and subsequently decreased immune function. Research indicates a bout of acute stress of any kind will cause a temporary decrease in immune system functioning, while chronic stress will result in continued decline in immunity. 
     Overwhelming evidence demonstrates virtually any type of stress has a detrimental effect on the ability to maintain optimal levels of natural killer (NK) cell cytotoxic activity. A severe life stress may be associated with up to a 50-percent reduction of NK-cell activity. Since NK-cell activity plays a vital role in immune system surveillance against viruses and cancer cells, a sustained decrease in this aspect of immune performance can have serious consequences. 
     A high degree of stress predicted a lowered ability of NK cells to destroy cancer cells and significantly predicted a poorer response to interventions aimed at improving NK-cell activity. Chronic stress preceding an acutely stressful event can significantly impact NK-cell activity. Chronic stress may result in a greater sense of subjective distress, higher peak levels of epinephrine, a more pronounced immediate reduction in NK-cell activity, and a protracted decline of NK-cell activity in the individuals. 
     The ability to produce secretory IgA (sIgA) also appears to be influenced by stress. sIgA may be the single-most important aspect of humoral immunity in the mucus secretions of the digestive system, mouth, lungs, urinary tract, and other body cavities, and any decline in its levels can decrease resistance to microbial pathogens. Higher levels of the catecholamine stress hormone epinephrine are significantly associated with lower sIgA concentrations. Daily problems, lack of a sense of humor, and negative emotions can decrease sIgA levels. 
     Stress has a significant influence on the balance of intestinal microflora. In a study it was noted that, the composition of the flora was not significantly affected by drastic changes in diet, but statistically significant shifts in the proportions of some species were noted in individuals under conditions of anger or fear stress. 
     Anti-stress activity of enteric coated purified Ashwagandha root extract enriched with withanolide glycosides and saponins, wherein the alkaloids, withanolide aglycones and oligosaccharides were removed from the extract, showed enhanced anoxia stress tolerance time. An enhanced anoxia stress tolerance time indicates more antistress activity. Anoxia stress tolerance time was enhanced in group after administering Ashwagandha root extract with 3.5% withanolide glycosides coated with Eudragit. 
     Enteric coated purified Ashwagandha root extract enriched with withanolide glycosides and saponins showed significant enhanced swimming endurance time. Alkaloids, withanolide aglycones and oligosaccharides were removed from purified Ashwagandha root extract enriched with withanolide glycosides and saponins. Enhancement in swimming endurance time is also found in groups after administering Ashwagandha root extract with 3.5% withanolide glycoside (50 mg/kg) and coated Ashwagandha root extract with 3.5% withanolide glycoside (50 mg/kg). Alkaloidal rich fraction of Ashwagandha root extract has very little effect on increasing the swimming time. 
     Enteric coated purified Ashwagandha root extract enriched with withanolide glycosides and saponins, and from which alkaloids, withanolide aglycones and oligosaccharides were removed, showed a significant increase in the bone marrow cells compared to control animals. Moreover, the number of α-esterase positive cells are also found to be increased significantly in the enteric coated purified Ashwagandha root extract treated groups. Ashwagandha extract with 3.5% withanolide glycoside and Ashwagandha extract with 3.5% withanolide glycoside coated with Eudragit showed increase in the bone marrow cells compared to control animals. Ashwagandha extract with 3.5% withanolide glycoside with and without coating also showed increase in number of α-esterase positive cells compared to controls. 
     Maximum antibody titre value is observed with enteric coated purified Ashwagandha root extract treated groups. Ashwagandha root extract with 3.5% withanolide glycoside coated with Eudragit also showed a high antibody titre value. The maximum number of plaque forming cells (PFC) is found after administering enteric coated Ashwagandha root extract enriched with withanolide glycosides. Alkaloids, withanolide aglycones and oligosaccharides were removed from the purified Ashwagandha root extract enriched with withanolide glycosides. Ashwagandha root extract with 3.5% withanolide glycosides also showed an increased number of plaque forming cells (PFC). 
     Anti-diabetic activity of Ashwagandha root extract enriched with withanolide glycosides and saponins with or without coating, wherein the alkaloids, withanolide aglycones and oligosaccharides were removed from the extract, in streptozotocin induced diabetic rats shows that enteric coated Ashwagandha extract has more antidiabetic activity at lower dosages compared to Ashwagandha extract without coating. Treatment with Ashwagandha extract with minimum 1% withanolide glycosides at 100 mg/kg dose reduced the FBG from 423 to 302 mg/dl whereas the same extract after coating with Eudragit reduced the FBG from 428 to 300 mg/dl. Ashwagandha root extract with 5% withanolide glycosides at 100 mg/kg reduced the FBG from 425 to 224 mg/dl whereas coated product reduced the level from 423 to 222 mg/dl. Ashwagandha extract with minimum 3.5% withanolide glycosides at 100 mg/kg and its coated product at 20 mg/kg dose reduced the FBG from 425 to 163 and 426 to 162 mg/dl respectively. The purified Ashwagandha root extract with 35% withanolide glycoside given at 100 mg/kg reduced the FBG level from 424 to 120 mg/dl whereas purified Ashwagandha root extract with 35% withanolide glycoside coated with Eudragit given at 20 mg/kg for 28 days reduced the FBG from 424 to 119 mg/dl. Purified Ashwagandha root extract with 80% withanolide glycoside at 100 mg/kg and its coated product at 20 mg/kg are most effective and reduced the FBG level from 426 to 73 and 425 to 72 mg/dl respectively. 
     Antidiabetic activity of coated Ashwagandha extracts in different doses are studied in streptozotocin induced diabetic rats. Ashwagandha extract with 3.5% withanolide glycosides coated with Eudragit at 1, 5, 10, 20 and 40 mg/kg daily dosage for 28 days reduced the FBG level to 301, 250, 202, 164 and 145 mg/dl respectively. Purified Ashwagandha root extract with 35% withanolide glycoside coated with Eudragit was the most effective in reducing the FBG levels in rats. This product at 1, 5, 10, 20 and 40 mg/kg daily dosage reduced the FBG level to 250, 200, 160, 120 and 80 mg/dl respectively. 
     Method of enhancing the endurance after administering enteric coated Ashwagandha extract in combination with Amaranth extract is provided. After feeding with Ashwagandha extract with 3.5% withanolide glycosides coated with Eudragit at 20 mg/kg increased the swimming endurance and immobility time got reduced to 77.33 seconds. The immobility time in rats fed with Amaranth extract with 9% nitrate content at 50 mg/kg was recorded as 66.67 seconds. 
     Feeding of rats with a 1:1 combination of Ashwagandha extract with 3.5% withanolide glycosides coated with Eudragit and Amaranth extract with 9% nitrate content is most effective and it reduced the immobility time to 52.67 seconds. 
     In another embodiment Ashwagandha extract is tested in human subjects and found to have useful for sleep disorders, enhancing endurance, old age rejuvenation, immunomodulatory effects and it is described by the following studies. 
     Anti Infertility Study: 
     A multi-center randomized, parallel-group, double-blind, placebo-controlled study was done in 60 healthy stressed adults suffering from Infertility for 60 days. 
     Male volunteers with symptoms of anxiety (Total baseline score of 17 or greater on Hamilton Anxiety Rating Scale), low sperm concentration (sperm count; sperm/ml)&lt;15 millions/ml; less sperm total motility was included in the study. Ashwagandha extract at a dose of 250 mg/day in 30 days improved the semen volume, improved Sperm motility with progressive sperm motility, increased total number of spermatozoa per ejaculate, improved sperm morphology, improved viability of spermatozoa, increased serum levels of total and free testosterone, reduced anxiety measured by HAM-A score and increased the number of spontaneous pregnancies. 
     Immunomodulatory Study: 
     A double-blinded randomized controlled trial for immunomodulatory effects of Ashwagandha ( Withania somnifera ) extract on 24 adult healthy volunteers was done at a dose of 250 mg/day for 60 days. There was a significant increase in the Th1 and Th2 cytokines after 30 days of Ashwagandha extract administration along with significant increase in T-helper cells and NK-cells. 
     Testosterone Study: 
     A double-blinded randomized placebo controlled trial to evaluate the effect of Ashwagandha extract 250 mg/day for 60 days on physical, sexual, vitality function and levels of testosterone was done in healthy adults. Volunteer were having serum testosterone levels that averaged less than 275 ng per deciliter. They also had self-reported decreased libido and a score of 20 or less on the sexual-desire domain (range, 0 to 33, with higher scores indicating greater desire) of the Derogatis Interview for Sexual Functioning in Men-II (DISF-M-II). They also reported difficulty walking or climbing stairs, reported low vitality and a score of less than 40 on the Functional Assessment of Chronic Illness Therapy (FACIT) Fatigue scale (range, 0 to 52, with higher scores indicating less fatigue). The volunteers on Ashwagandha extract showed improvement in Sexual Function shown as an improvement in total score on the Psychosexual Daily Questionnaire (PDQ-Q4); Improvement in physical function showed by increase in the percentage of men who had an increase of at least 50 m in the distance walked during the 6-minute walk test (6-MWT); Improvement in Vitality Function showed as increase in the percentage of men who had an increase of at least 4 points in the score on the Functional Assessment of Chronic Illness Therapy (FACIT)—Fatigue scale; Improvement in the erectile-function domain (range, 0 to 30, with higher scores indicating better function) of the International Index of Erectile Function (IIEF); Improvement in score on the sexual-desire domain of the DISF; 
     Increase in Change in serum Testosterone and Free-testosterone levels within 30 days of treatment. 
     Anti Anxiety Study: 
     A randomized, double-blind, placebo-controlled study evaluating the efficacy, safety, tolerability, and pharmacological actions of an Ashwagandha ( Withania somnifera ) extract was done in healthy, stressed adult volunteers. 60 healthy subjects with a total score between 6 to 17 on the Hamilton Rating Scale of Anxiety was given 250 mg/day of Ashwagandha extract for 60 days. There was significant improvement in total score on Hamilton Anxiety Rating Scale (HAM-A), improvement in total score and subscale scores on the Depression Anxiety Stress Scale (DASS-21), reduction in blood cortisol and DHEA-s levels and improvement in testosterone levels within 30 days. 
     Various methods for the preparation of the extract enriched with withanolide glycosides content and without alkaloid content prepared by the extraction of root of Ashwagandha are as under: 
       FIG.  1    describes a method of preparation of Ashwagandha root powder after ammonia treatment and MDC extraction. Fresh root of Ashwagandha is treated with 2% ammonia solution in ratio of 1:2 (2% ammonia:root) for 4 hrs. The ammonia treated Ashwagandha root is extracted with methylene dichloride (dichloro methane) at a temperature of 70-80° C. in a Soxhlet extractor for 10 hrs to form residue and filtrate. The filtrate and residue is separated. The residue is washed with water till the pH become neutral. Then dried the residue in vacuum oven at 90-100° C. After drying the residue (root of Ashwagandha) is powdered to form a powder of root of Ashwagandha. 
     The filtrate is concentrated in an Agitated thin film evaporator (ATFE) at a temperature of 40-50° C. to form concentrated dichloro methane (MDC) extract. Concentrated MDC extract is dried under vacuum at above 500 mm of mercury to form powder of “dichloromethane extract” of Ashwagandha. 
       FIG.  2    describes a method of preparation of 60% methanol extract of Ashwagandha root extract after ammonia treatment and MDC extraction. Fresh root of Ashwagandha is treated with 2% ammonia solution in ratio of 1:2 (2% ammonia:root) for 4 hrs. The ammonia treated Ashwagandha root is extracted with methylene dichloride (dichloro methane) at a temperature of 70-80° C. in a Soxhlet extractor for 10 hrs to form residue and filtrate. The filtrate and residue is separated. The residue is washed with water till the pH become neutral. Then dried the residue in vacuum oven at 90-100° C. After drying the residue (root of Ashwagandha) is powdered to form a powder of root of Ashwagandha. 
     Powder of root of Ashwagandha is extracted with 60% methanol for one hour. The root and methanol part (supernatant) obtained are separated. Supernatant is concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract is dried under vacuum at above 500 mm of mercury to get powder of 60% methanol extract of Ashwagandha. 
       FIG.  3    describes a method of preparation of 50% methanol extract of Ashwagandha root extract. Root of Ashwagandha is extracted with 50% methanol for one hour. The root and methanol part (supernatant) obtained are separated. Supernatant is concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract is dried under vacuum at above 500 mm of mercury to get powder of 50% methanol extract of Ashwagandha. 
     Powder of 50% methanol extract of Ashwagandha is dissolved in minimum amount of methanol. The dissolved methanol part is concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. Acetone is added dropwise to the concentrated methanol extract until the precipitation is complete. Then, the precipitate was filtered and dried under vacuum at above 500 mm of mercury to get powder of Ashwagandha extract. 
       FIG.  6    describes a method of preparation of chloroform insoluble extract of 60% methanol extract of Ashwagandha root extract. Roots of Ashwagandha is extracted with 60% methanol for one hour. The root and methanol part (supernatant) obtained are separated. Supernatant is concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract is dried under vacuum at above 500 mm of mercury to get powder of 60% methanol extract of Ashwagandha. 
     Powder of 60% methanol extract of Ashwagandha is refluxed with chloroform at the boiling temperature (70-80° C.) of chloroform for half an hour. The residue (chloroform insoluble) and supernatant (chloroform soluble) are separated. The residue is dried under vacuum at above 500 mm of mercury to get powder of chloroform extract of 60% methanol extract of Ashwagandha. 
       FIG.  4    describes a method of preparation of purified methanol extract of Ashwagandha root extract after ammonia treatment and MDC extraction. Fresh root of Ashwagandha is treated with 2% ammonia solution in ratio of 1:2 (2% ammonia:root) for 4 hrs. The ammonia treated Ashwagandha root is extracted with methylene dichloride (dichloro methane) at a temperature of 70-80° C. in a Soxhlet extractor for 10 hrs to form residue and filtrate. The filtrate and residue is separated. The residue (Ashwagandha root) is washed with water till the pH become neutral. Then dried the residue (Ashwagandha root) in vacuum oven at 90-100° C. After drying the residue (root of Ashwagandha) is powdered to form a powder of root of Ashwagandha. (Sample 1). 
     Powder of roots of Ashwagandha (sample 1) is extracted with 60% methanol for one hour. The residue and supernatants obtained are separated. Supernatant is concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract is dried under vacuum at above 500 mm of mercury to get powder of 60% methanol extract of Ashwagandha. (Sample 2) 
     Sample 2 is dissolved in water and clarify. The supernatant obtained after clarification is loaded on a HP20 resin column. Column is initially eluted with water followed by 50% methanol. 50% methanol fraction is collected and concentrated in an Agitated thin film evaporator (ATFE) to form concentrated methanol extract. Concentrate methanol fraction is fed into vacuum stripper and dried under vacuum at above 500 mm of mercury to get powder of 50% methanol extract (sample 3) of Ashwagandha. 
       FIG.  5    describes a method of preparation of purified 60% methanol extract of Ashwagandha root extract after ammonia treatment and MDC extraction. Fresh root of Ashwagandha is treated with 2% ammonia solution in ratio of 1:2 (2% ammonia:root) for 4 hrs. The ammonia treated Ashwagandha root is extracted with methylene dichloride (dichloro methane) at a temperature of 70-80° C. in a Soxhlet extractor for 10 hrs to form residue and filtrate. The filtrate and residue is separated. The residue (Ashwagandha root) is washed with water till the pH become neutral. Then dried the residue (Ashwagandha root) in vacuum oven at 90-100° C. After drying the residue (root of Ashwagandha) is powdered to form a powder of root of Ashwagandha. (Sample 1) 
     Powder of roots of Ashwagandha (sample 1) is extracted with 60% methanol for one hour. The residue and supernatants obtained are separated. Supernatant is concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract is dried under vacuum at above 500 mm of mercury to get powder of 60% methanol extract of Ashwagandha. (Sample 2) 
     Sample 2 is dissolved in water and clarify. The supernatant obtained after clarification is loaded on a HP20 resin column. Column is initially eluted with water followed by 50% methanol. 50% methanol fraction is collected and concentrated in an Agitated thin film evaporator (ATFE) to form concentrated methanol extract. Concentrate methanol fraction is fed into vacuum stripper and dried under vacuum at above 500 mm of mercury to get powder of 50% methanol extract (sample 3) of Ashwagandha. 
     Sample 3 is dissolved in water and centrifuged at 10,000 rpm for 10 minutes to form supernatant and residue. Supernatant is loaded on the C18 column. After passing the supernatant through the pre conditioned column, column is eluted with 10% methanol, 50% methanol and 100% methanol. All the methanol parts were collected. Each collected part is separately concentrated in an Agitated thin film evaporator (ATFE) to form concentrated extract of each part. Concentrate of each part is fed into vacuum stripper and dried separately under vacuum at above 500 mm of mercury to form powder of purified 10% methanol elute, 50% methanol elute (sample 4) and 100% methanol elute of Ashwagandha root. 
     Sample 4 is dissolved in minimum amount of methanol. The dissolved sample 4 in methanol was concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract of sample 4. Acetone is added dropwise to the concentrated methanol extract of sample 4 until the precipitation is complete. Then, the precipitate is filtered and dried under vacuum at above 500 mm of mercury to get powder of Ashwagandha extract (Sample 5). 
     In some embodiments, chloroform extract of Ashwagandha is provided. Another embodiments provides 100% methanol extract, 80% methanol extract and chloroform-methanol extract of Ashwagandha. 
     In another embodiment, root of Ashwagandha is extracted with 100% methanol for one hour. The root and methanol part (supernatant) obtained are separated. Supernatant is concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract is dried under vacuum at above 500 mm of mercury to get powder of 100% methanol extract of Ashwagandha. 
       FIG.  7    describes a method of preparation of 20% ethanol extract of Ashwagandha root extract after ammonia treatment and MDC extraction. Fresh root of Ashwagandha is treated with 2% ammonia solution in ratio of 1:2 (2% ammonia:root) for 4 hrs. The ammonia treated Ashwagandha root is extracted with methylene dichloride (dichloro methane) at a temperature of 70-80° C. in a Soxhlet extractor for 10 hrs to form residue and filtrate. The filtrate and residue is separated. The residue is washed with water till the pH become neutral. Then dried the residue in vacuum oven at 90-100° C. After drying the residue (root of Ashwagandha) is powdered to form a powder of root of Ashwagandha. 
     Powder of root of Ashwagandha is extracted with 20% ethanol for one hour. The ethanol part (supernatant) obtained is concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated ethanol extract. The concentrated methanol extract is filtered to form a filtrate. 35% maltodextrin is added to the filtrate and spray dried to get powder of 20% ethanol extract of Ashwagandha. 
     In one embodiment fresh roots of Ashwagandha are cleaned. Cleaned roots are dried and pulverized to get powder of root Ashwagandha. 
       FIG.  8    describes a method of preparation of purified Amaranth extract. Fresh leaves and stem of Amaranth is cleaned, crushed and extracted for about 1 hr using water in an extractor with reflux condenser to obtain residue and supernatant. The residue and supernatant is separated by draining out the supernatant from the extractor bottom through the filter cloth. The resultant supernatant is concentrated in an Agitated thin film evaporator (ATFE) at a temperature of 85° C. to form concentrated extract. Concentrated water extract is passed through a carbon column to obtain a filtrate. Filtrate is concentrated and clarified to form a supernatant and residue. Supernatant is dried under vacuum at above 500 mm of mercury to get powder of purified water extract of fresh Amaranth. 
       FIG.  9    describes a method of preparation of combination of Enteric coated Ashwagandha extract and Amaranth extract. Enteric coated Ashwagandha extract and Amaranth extract is blended in 1:1 ratio by using Double Cone Blender. 
     In another embodiment Ashwagandha extract or powder in different pH is prepared by adding Ashwagandha powder/extract to different buffer (pH 1, 2, 3, 5, 7 and 7.4) in 1:20 ratio of Ashwagandha powder/extract:buffer. The extract-buffer solution is kept in a water bath at 37° C. for 2 hrs. The solution is neutralised by adding base or acid solution. The neutralised solution is concentrated and dried. 
     Details of some of the trials/experiments carried out and findings are explained below by way of examples. 
     Example 1 
     Fresh roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were treated with 2% ammonia solution in a ratio of 1:2 solvent:roots of Ashwagandha for 4 hrs. Ammonia treated Ashwagandha roots were filled in the Soxhlet extractor and extracted with dichloromethane or methylene dichloride (MDC) (300 L). The extraction was carried out for 10 hrs at a temperature of about 70° C. After the completion of extraction, the supernatant and residue were separated by filtration. Residue (roots of Ashwagandha) after MDC extraction was washed with water till the pH become neutral. Then dried the root in a vacuum oven at 90-100° C. After drying the roots of Ashwagandha was powdered to form a powder of roots of Ashwagandha (sample 1) (Yield 88%). 
     The supernatant was concentrated in an Agitated thin film evaporator (ATFE) at a temperature of 70° C. to form concentrated MDC extract. Concentrated MDC extract was dried under vacuum at above 500 mm of mercury to get powder of MDC extract of Ashwagandha after ammonia treatment (sample 2) (Yield 0.6%). 
     See also extract preparation in  FIG.  1   . 
     The alkaloid content in powder of root of Ashwagandha after ammonia treatment (sample 1) was found to be 0.6% by gravimetry method. The withanolide content in powder of root of Ashwagandha after ammonia treatment (sample 1) was about 0.7% by HPLC method. The withanolide glycoside content in powder of root of Ashwagandha after ammonia treatment (sample 1) was about 4% by HPLC method. Saponin content in powder of root of Ashwagandha after ammonia treatment (sample 1) was about 1% by UV method. The alkaloid content in powder of MDC extract of ammonia treated Ashwagandha root (sample 2) was about 60% by gravimetry method. 
     Example 2 
     Fresh roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were treated with 2% ammonia solution in a ratio of 1:2 solvent:roots of Ashwagandha for 4 hrs. Ammonia treated Ashwagandha roots were filled in the Soxhlet extractor and extracted with dichloromethane or methylene dichloride (MDC) (300 L). The extraction was carried out for 10 hrs at a temperature of about 70° C. After the completion of extraction, the supernatant and residue were separated by filtration. Residue (roots of Ashwagandha) after MDC extraction was washed with water till the pH become neutral. Then dried the root in a vacuum oven at 90-100° C. After drying the roots of Ashwagandha was powdered to form a powder of roots of Ashwagandha (sample 1) (88%). 
     88 Kg of powder of roots of Ashwagandha (sample 1) was extracted with 60% methanol. Sample 1 was refluxed with 60% methanol (300 L) at the boiling temperature (60-70° C.) of methanol for one hour to obtain a second residue and second supernatant. The second residue was then further extracted two more times with three times the quantity of methanol at each time. The residue and supernatants were separated. All the supernatants were pooled and concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract was dried under vacuum at above 500 mm of mercury to get powder of 60% methanol extract of Ashwagandha. The yield was about 10% (Sample 2). 
     See also extract preparation in  FIG.  2   . 
     The withanolide glycoside content in powder of ammonia treated 60% methanol extract of Ashwagandha was about 6% by HPLC. Saponin content in powder of ammonia treated 60% methanol extract of Ashwagandha was about 1.5% by UV method. Alkaloid content in powder of ammonia treated 60% methanol extract of Ashwagandha was about 0.1% by gravimetry method. Withanolide aglycones content in powder of ammonia treated 60% methanol extract of Ashwagandha was about 0.2% by HPLC. Oligosaccharide content in in powder of ammonia treated 60% Methanol extract of Ashwagandha was about 5% by HPLC. 
     Example 3 
     Roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were extracted with 50% methanol. Roots were refluxed with 50% methanol at the boiling temperature (60-70° C.) of methanol for one hour to obtain a first residue and first supernatant. The first residue was then further extracted two more times with four times the quantity of methanol at each time. The residue and supernatants were separated. All the supernatants were pooled and concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract was dried under vacuum at above 500 mm of mercury to get powder of 50% methanol extract of Ashwagandha. (yield 22%) (Sample 1). 
     Powder of 50% methanol extract of Ashwagandha was dissolved in minimum amount of methanol. The dissolved methanol part was concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. Acetone was added dropwise to the concentrated methanol extract until the precipitation was complete. Then, the precipitate was filtered and dried under vacuum at above 500 mm of mercury to get powder of Ashwagandha extract (yield of about 3%) (Sample 2). 
     See also extract preparation in  FIG.  3   . 
     Withanolide glycoside content in powder of 50% methanol extract of Ashwagandha (Sample 2) was 20% by HPLC. Saponin content in powder of 50% methanol extract of Ashwagandha (Sample 2) was 3.5% by UV method. Alkaloid content in powder of 50% methanol extract of Ashwagandha (Sample 2) was 1.2% by gravimetry method. Withanolide aglycones content in powder of 50% methanol extract of Ashwagandha (Sample 2) was about 1% by HPLC method. 
     Example 4 
     Roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were treated with 2% ammonia solution in a ratio of 1:2 solvent:roots of Ashwagandha for 4 hrs. Ammonia treated Ashwagandha roots were filled in the Soxhlet extractor and extracted with dichloromethane or methylene dichloride (MDC) (300 L). The extraction was carried out for 10 hrs at a temperature of about 70° C. After the completion of extraction, the first supernatant and first residue (roots of Ashwagandha) formed was separated by filtration. First residue (roots of Ashwagandha after MDC extraction) was washed with water till the pH become neutral. Then dried the first residue (root of Ashwagandha) in a vacuum oven at 90-100° C. After drying the first residue (roots of Ashwagandha) was powdered to form a powder of roots of Ashwagandha (Yield 88%) after ammonia treatment (sample 1). 
     88 Kg of powder of roots of Ashwagandha (sample 1) was extracted with 60% methanol. Sample 1 was refluxed with 60% methanol (300 L) at the boiling temperature (60-70° C.) of methanol for one hour to obtain a second residue and second supernatant. The second residue was then further extracted two more times with three times the quantity of methanol at each time. The residue and supernatants were separated. All the supernatants were pooled and concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract was dried under vacuum at above 500 mm of mercury to get powder of 60% methanol extract of Ashwagandha. (yield 10%) (Sample 2). 
     10 Kg of sample 2 was dissolved in water and clarified to form supernatant and residue. Supernatant was loaded on a HP20 resin column. After passing the supernatant through the column, column was initially eluted with water followed by 50% methanol. 50% methanol elute was collected. 50% methanol fraction was concentrated in an Agitated thin film evaporator (ATFE) to form concentrated methanol extract. Concentrate fraction was fed into vacuum stripper and dried under vacuum at above 500 mm of mercury to get powder of 50% methanol extract (sample 3)(yield 8%) of Ashwagandha. The powder of 50% methanol fraction of Ashwagandha contain withanolide glycosides (35%). 
     See also extract preparation in  FIG.  4   . 
     The withanolide glycoside content in powder of 50% methanol elute of Ashwagandha after column treatment (sample 3) was found to be 35% by HPLC. Saponin content in powder of 50% methanol elute of Ashwagandha after column treatment (sample 3) was 10% by UV method. The alkaloid content in powder of 50% methanol elute of Ashwagandha after column treatment (sample 3) was found to be 0.04% by gravimetry method. The withanolide aglycone content in powder of 50% methanol elute of Ashwagandha after column treatment (sample 3) was not detectable by HPLC. Oligosaccharides in powder of 50% methanol elute of Ashwagandha after column treatment (sample 3) were not detectable. 
     Example 5 
     Roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were treated with 2% ammonia solution in a ratio of 1:2 solvent:roots of Ashwagandha for 4 hrs. Ammonia treated Ashwagandha roots were filled in the Soxhlet extractor and extracted with dichloromethane or methylene dichloride (MDC) (300 L). The extraction was carried out for 10 hrs at a temperature of about 70° C. After the completion of extraction, the first supernatant and first residue was separated by filtration. First residue (roots of Ashwagandha after MDC extraction) was washed with water till the pH become neutral. Then dried the first residue (roots of Ashwagandha) in a vacuum oven at 90-100° C. After drying the first residue (roots of Ashwagandha) was powdered to form a powder of roots of Ashwagandha (Yield 88%) after ammonia treatment (sample 1). 
     88 Kg of powder of roots of Ashwagandha (sample 1) was extracted with 60% methanol. Sample 1 was refluxed with 60% methanol (300 L) at the boiling temperature (60-70° C.) of methanol for one hour to obtain a second residue and second supernatant. The second residue was then further extracted two more times with three times the quantity of methanol at each time. The residue and supernatants were separated. All the supernatants were pooled and concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract was dried under vacuum at above 500 mm of mercury to get powder of 60% methanol extract of Ashwagandha. (yield 10%) (Sample 2). 
     10 Kg of sample 2 was dissolved in water and clarified to form supernatant and residue. Supernatant was loaded on a HP20 resin column. After passing the supernatant through the column, column was initially eluted with water followed by 50% methanol. 50% methanol elute was collected. 50% methanol elute was concentrated in an Agitated thin film evaporator (ATFE) to form concentrated methanol extract. Concentrate fraction was fed into vacuum stripper and dried under vacuum at above 500 mm of mercury to get powder of 50% methanol extract (sample 3)(yield 8%) of Ashwagandha. The powder of 50% methanol fraction of Ashwagandha contain withanolide glycosides (35%). 
     Powder of 50% methanol fraction (sample 3) was dissolved in water and centrifuged at 10,000 rpm for 10 minutes to form supernatant and residue. Supernatant was loaded on the C18 column. Before passing through column, the column was conditioned with 10% methanol. After passing the supernatant through the pre conditioned column, column was eluted with 10% methanol, 50% methanol and 100% methanol. All the methanol parts were collected. Each collected part was separately concentrated in an Agitated thin film evaporator (ATFE) to form concentrated extract of each part. Concentrate of each part was fed into vacuum stripper and dried separately under vacuum at above 500 mm of mercury to form powder of purified 10% methanol elute (Yield 7%), 50% methanol elute (Yield 3.5%) (sample 4) and 100% methanol elute (Yield 1.5%) of Ashwagandha root. 
     The powder of 50% methanol fraction of Ashwagandha contain withanolide glycosides (50%), Alkaloids (0.01%) and withanolide aglycones (0.03%). 
     Powder of 50% methanol elute of root of Ashwagandha (sample 4) was dissolved in minimum amount of methanol. The dissolved sample 4 in methanol was concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract of sample 4. Acetone was added dropwise to the concentrated methanol extract of sample 4 until the precipitation was complete. Then, the precipitate was filtered and dried under vacuum at above 500 mm of mercury to get powder of Ashwagandha extract (Sample 5) (yield 1.8%). 
     See also extract preparation in  FIG.  5   . 
     The withanolide glycoside content in powder of column purified Ashwagandha extract (sample 5) was found to be 80% by HPLC. Saponin content in powder of column purified Ashwagandha extract (sample 5) was found to be 15% by UV method. The alkaloid content in powder of column purified Ashwagandha extract (sample 5) was found to be 0.001% by gravimetry method. The withanolide aglycone content in powder of column purified Ashwagandha extract (sample 5) was not detected by HPLC. Oligosaccharide content in powder of column purified Ashwagandha extract (sample 5) was not detected. 
     HPLC and LCMS Analysis of Withanolide Glycosides and Withanolide Aglycones 
     Extract of Ashwagandha (sample 5) was analysed by HPLC and found to contain withanolide glycosides (withanoside I to VII), sitoindosides (sitoindosides I to X) and withanamides and the presence was confirmed by LCMS analysis. 
     HPLC analysis of extract of Ashwagandha detects withanoside I to VII (withanolide glycosides), sitoindosides I to X and withanamides and negligible amount of withanolide aglycones (withaferin A, 12-deoxywithastramonolide, withanolide A) at 227 nm. 
     The extract of Ashwagandha was subjected to liquid chromatography-mass spectrometry (LCMS) analysis and the presence of active components like withanoside I to VII, sitoindosides I to X and withanamides were confirmed by LCMS data obtained. From LCMS data we can also confirmed the presence of negligible amount of inactive components like withanolide aglycones in the Ashwagandha extract. 
     Acid Hydrolysis 
     Ashwagandha extract (sample 5) was subjected to acid hydrolysis. 2.5 Kg of Sample 5 was dissolved in 1N hydrochloric acid by continuous stirring up to 4 hours. Then transferred the mixture in to a separating funnel and extracted with chloroform. The two layers were separated. Acid layer was removed and chloroform layer was collected. The chloroform layer was again washed with water in a separating funnel until pH become neutral. The chloroform layer was collected and filtered through a filter paper containing sodium sulphate. Filtered chloroform layer was concentrated and dried under vacuum at above 500 mm of mercury to get powder of Ashwagandha extract (sample 6) (yield 1.5%). 
     The extract after hydrolysis was analysed by HPLC. From the HPLC chromatogram at 227 nm the major peak obtained represents the inactive aglycone part. Only a small percentage of active glycosidic part was present. After hydrolysis of extract of Ashwagandha, the withanolide glycosides present in the extract was converted to aglycones and it was confirmed by LCMS. The extract of Ashwagandha after hydrolysis was subjected to liquid chromatography-mass spectrometry (LCMS) analysis and the presence of inactive components like withaferin A, 12-deoxywithastramonolide, withanolide A (molecular mass 471.54, 471, 471.64 respectively) were confirmed by LCMS. So it was again confirmed the presence of withanolide glycosides, sitoindosides and withanamides in the extract which was converted into aglycone part after hydrolysis. 
     Example 6 
     Roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were extracted with 60% methanol. Roots were refluxed with 60% methanol at the boiling temperature (60-70° C.) of methanol for one hour to obtain a first residue and first supernatant. The first residue was then further extracted two more times with four times the quantity of methanol at each time. The residue and supernatants were separated. All the supernatants were pooled and concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract was dried under vacuum at above 500 mm of mercury to get powder of 60% methanol extract of Ashwagandha. (Sample 1) (yield 18%). 
     18 Kg of sample 1 was refluxed with chloroform at the boiling temperature (70-80° C.) of chloroform for half an hour to obtain second residue and second supernatant. The second residue was then further extracted two more times with four times the quantity of chloroform at each time. The residue and supernatants were separated. The residue was dried under vacuum at above 500 mm of mercury to get powder of chloroform extract of methanol extract of Ashwagandha (Sample 2) (Yield 16%). 
     See also extract preparation in  FIG.  6   . 
     The withanolide content in powder of chloroform extract of 60% methanol extract of Ashwagandha (sample 2) was found to be 0.7% by HPLC method. Alkaloid content in powder of chloroform extract of 60% methanol extract of Ashwagandha (sample 2) was found to be 1.5% by gravimetry method. Withanolide glycoside content in powder of chloroform extract of 60% methanol extract of Ashwagandha (sample 2) was found to be 5% by HPLC method. Saponin content in powder of chloroform extract of 60% methanol extract of Ashwagandha (sample 2) was found to be 1.6% by UV method. Oligosaccharide content in in powder of chloroform extract of 60% methanol extract of Ashwagandha (sample 2) was found to be 20% by HPLC method. 
     Example 7 
     Analysis of Oligosaccharides, Withanolide Glycosides and Aglycones in Different Ashwagandha Extracts 
     Different extracts of Ashwagandha were analysed for the presence of oligosaccharides, withanolide glycosides and aglycones. 
     Extracts of Ashwagandha analysed were 
     1. 60% methanol extract of Ashwagandha root prepared as per Example 6; 
     2. Powder of ammonia treated 60% methanol extract of Ashwagandha prepared as per example 2; 
     3. Powder of 50% methanol fraction of Ashwagandha after passing through HP20 resin column prepared as per example 4; 
     4. Powder of column purified Ashwagandha extract prepared as per example 5. 
     HPTLC Analysis of Oligosaccharides and Withanolide Glycosides and Aglycones 
     All the above extracts were analysed by HPTLC for the presence of actives in the corresponding extract. 
     HPTLC was performed on precoated TLC plates. Standard solution of glycowithanolides (Sitoindosides IV and V), withanolide aglycones (withaferin A, 12-deoxywithastramonolide, withanolide A) and oligosaccharides (Sucrose and maltose) along with extracts of Ashwagandha roots were applied. The plates were developed using n-butanol-acetic acid-water 4:1:2 (v/v/v) as mobile phase. Densitometric evaluation of the plates was performed at λ=225 nm. 
     After the analysis of different Ashwagandha extracts, Powder of 50% methanol fraction of Ashwagandha after passing through HP20 resin column prepared as per example 4 and Powder of column purified Ashwagandha extract prepared as per example 5 showed the presence of withanolide glycosides (R f  0.43-0.78). But there is no spot observed for the presence of withanolide aglycones and oligosaccharides. But in powder of ammonia treated 60% Methanol extract of Ashwagandha prepared as per example 2, the HPTLC pattern showed the presence of oligosaccharides (R f  0.24-0.38). In the HPTLC pattern of 60% methanol extract of Ashwagandha extract, the intensity of spot between R f  0.24-0.38 corresponds to oligosaccharides and other two spots corresponds to withanolide glycosides (R f  0.43-0.78) and withanolide aglycones (R f  0.82-0.94). 
     HPLC Analysis of Oligosaccharides and Withanolide Glycosides and Aglycones 
     Oligosaccharides were determined using Waters HPLC system with a RI detector and Empower software with a carbohydrate analysis column [Waters] 300×3.9 mm; acetonitrile:water—80:20 (v/v) was used as the mobile phase; the run time was 10 minutes and flow rate was 2 ml/min in an isocratic mode. 
     The withanolide glycosides/withanolide aglycones were analyzed by high performance liquid chromatography (HPLC) on a RP C18 column (250×4.6 mm Shimadzu Co., Japan.) using 0.01M anhydrous potassium dihydrogen orthophosphate and Acetonitrile as the mobile phase and UV detection at 227 nm. The eluent flow rate was 1 ml/min. 
     HPLC analysis of 60% methanol extract of Ashwagandha root showed about 5% withanolide glycosides, 0.7% withanolide aglycones and 20% oligosaccharides. Powder of ammonia treated 60% methanol extract of Ashwagandha contained about 6% withanolide glycosides, about 0.2% withanolide aglycones and about 5% oligosaccharides. The weight ratio of withanolide glycosides to withanolide aglycones was 30:1 and weight ratio of withanolide glycosides to oligosaccharide was 6:5. Oligosaccharides were not detected in the enriched extracts of Ashwagandha prepared as per Example 4 (about 35% withanolide glycosides). Oligosaccharides were not detected in the enriched extracts of Ashwagandha prepared as per Example 5 (about 80% withanolide glycosides). 
     Example 8 
     Method of Making Enteric Coated Extract 
     Specific quantity of purified Ashwagandha root extract/other Ashwagandha root extracts were loaded into the bowl of the fluid bed extractor (pam glatt pharma technologies). The bowl has a fine Stainless steel mesh at the bottom. The air used for drying/fluidizing was successively filtered through HEPA (High-efficiency particulate arrestance) filters (EU 13 grade, 0.3 micron rating, 99.997% efficiency). 
     Hot, filtered air up to 90° C. was passed at high velocity from the bottom of the FBE bowl through the feed material (Purified Ashwagandha root extract/Ashwagandha root extract) and feed material was fluidised. 
     Meantime, enteric/nonenteric coating material was dissolved in specific quantity of solvents depends on the nature of enteric/nonenteric coating material. Enteric/nonenteric coating solution was sprayed into fluidised material by using a spraying devise attached to the FBE (spray speed 2 L in 1 Hr, pump rpm range 10-12). Through the process of fluid bed coating, fluidized particles are continuously sprayed with coating solution, depositing layers (films) of material to the surface of the particles, and yielding an even layer with particular thickness. 
     Example 9 
     The tablets were prepared on an automated 16 station tablet punch machine. The tablets were coated by Pan coating method. In brief, 5 Kg tablets were introduced into the Pan and rotated at 20 rpm. Coating material was sprayed on to the tablets via a spray gun as 30% aqueous dispersion. Simultaneously hot blower (110 degree) was started for fast drying of the tablets. Tablets were coated till a weight gain of 5-6%. After coating, tablets were further kept in hot air stream for 15 minutes to ensure complete drying. 
     Example 10 
     Method of Analysis of Withanolide Glycoside and Withanolide Aglycones 
     125 mg of Ashwagandha extract was accurately weighed and transferred into a 25 ml standard flask and made up to a 25 ml solution with methanol. Filtered through 0.2 μm membrane filter before injection. Standard was prepared by weighing accurately 5 mg standard [standards from Chromadex] and transferred into a 5 ml standard flask and made up to a 5 ml solution with methanol. From this pipette out 200 μl into 10 ml standard flask (20 ppm) and made up to a 10 ml solution with methanol. Filter through 0.2 μm membrane filter before injection. 
     The withanolide glycosides/withanolide aglycones were analyzed by high performance liquid chromatography (HPLC) on a RP C18 column (250×4.6 mm Shimadzu Co., Japan.) using 0.01M anhydrous potassium dihydrogen orthophosphate (pH was adjusted to 2.58 using 10% orthophosphoric acid solution) and acetonitrile as the mobile phase and UV detection at 227 nm. The eluent flow rate was 1 ml/min. 
     By comparing the area of standard and sample, the percentage of withanolide glycosides/withanolide aglycones were calculated using the formula 
     
       
         
           
             
               
                 
                   
                     % 
                     ⁢ 
                     
                       
                           
                       
                       ⁢ 
                       
                           
                       
                     
                     ⁢ 
                     of 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     withanolide 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     
                       glycosides 
                       / 
                     
                   
                 
               
               
                 
                   
                     withanolide 
                     ⁢ 
                     
                         
                     
                     ⁢ 
                     aglycones 
                   
                 
               
             
             = 
             
               
                 
                   
                     
                       Area 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       of 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       sample 
                       × 
                       amount 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       of 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       std 
                       × 
                     
                   
                 
                 
                   
                     
                       Purity 
                       ⁢ 
                       
                         
                             
                         
                         ⁢ 
                         
                             
                         
                       
                       ⁢ 
                       of 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       std 
                     
                   
                 
               
               
                 Area 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 of 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 Std 
                 × 
                 weight 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 of 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 the 
                 ⁢ 
                 
                     
                 
                 ⁢ 
                 sample 
               
             
           
         
       
     
     Example 11 
     Method of Analysis of Alkaloids 
     3 g of dried extract of Ashwagandha (W1) was poured into the conical flask. 5 ml ammonia was added to the extract in conical flask and shaken for 15 minutes. Then mixture of 75 ml of ether and 25 ml of alcohol was added to the flask. This solution was shaken continuously for 1 hour. The solution was filtered in the separator through filter paper. The residue was washed from conical flask with mixture of 75 ml of ether and 25 ml of alcohol. The total solution was extracted with 25 ml dilute sulphuric acid in a separating funnel. The two layers were separated. Ether layer was removed and acid aqueous layer was collected. Acid aqueous layer was washed with 25 ml of methylene dichloride (MDC) in a separating funnel. Two layers were separated and acid aqueous layer was collected and adjusted to alkaline media with ammonia. This aqueous solution was extracted with 25 ml chloroform in a separating funnel. Two layers formed were separated. Chloroform layer was collected and 25 ml chloroform was again added to aqueous layer. This process was repeated two more times and all the chloroform layers were collected. Chloroform part was washed with water in a separating funnel. After separation of two layers, chloroform layer was collected and filtered into a weighed beaker (W2). Evaporated to dryness and then weighed the beaker with dried sample (W3). 
     Calculation 
     
       
         
           
             
               % 
               ⁢ 
               
                   
               
               ⁢ 
               of 
               ⁢ 
               
                   
               
               ⁢ 
               alkaloid 
             
             = 
             
               
                 
                   ( 
                   
                     
                       W 
                       ⁢ 
                       3 
                     
                     - 
                     
                       W 
                       ⁢ 
                       2 
                     
                   
                   ) 
                 
                 × 
                 100 
               
               
                 W 
                 ⁢ 
                 1 
               
             
           
         
       
     
     (See, Anonymous. (1996). Indian Pharmacopoeia, Vol. II, Government of India, Ministry of Health and Family Welfare, New Delhi, A-81-83, 95, 736.) 
     Example 12 
     Dissolution Study 
     The release pattern of study drug was tested in simulated gastric fluid (pH 1.2) and simulated intestinal fluid from (pH 6.8) by in vitro USP dissolution apparatus (LABINDIA DS 8000). The dissolution medium (simulated gastric fluid without enzyme, pH 1.2), free from dissolved air, was filled into the vessel of the dissolution apparatus. Apparatus was assembled and dissolution medium was heated to 36.5° to 37.5°. The enteric coated tablet containing alkaloid free purified Ashwagandha root extract was sunk to the bottom of one vessel prior to the rotation of the paddle. In another vessel tablet of alkaloid free purified Ashwagandha root extract without enteric coating was sunk to the bottom of vessel prior to the rotation of the paddle. A suitable device such as a wire of glass helix was used to keep horizontal at the bottom of the vessel tablets that would otherwise float. 
     After two hours of operation, an aliquot of the liquid was collected from two vessels and the dissolution medium was changed to simulated intestinal fluid with pH 6.8 and repeated the above process. Samples were collected at 3, 4 and 5 hours. 
     The samples from two vessels at each time point were transferred into a liquid-liquid extractor and extracted with chloroform-methanol mixture (80:20). Acidic and chloroform-methanol phases were separated. Chloroform-methanol phase was collected and acidic phase was again extracted with chloroform-methanol two more times. All the chloroform-methanol phases were pooled and extracted with water. Aqueous and chloroform-methanol phases were separated and chloroform-methanol phase was collected. chloroform-methanol phase was concentrated and dried to form powder of chloroform-methanol extract. 
     chloroform-methanol extract is dissolved in methanol and analysed by HPLC. 
     
       
         
           
               
               
            
               
                   
               
               
                   
                 Percentage of withanolide glycosides 
               
            
           
           
               
               
               
            
               
                   
                 Enteric coated tablet of alkaloid 
                 Tablet of purified Ashwagandha 
               
               
                 Time 
                 free purified Ashwagandha  
                 root extract without  
               
               
                 (Hrs ) 
                 root extract 
                 enteric coating 
               
               
                   
               
            
           
           
               
               
               
            
               
                 0 
                 0 
                 0 
               
               
                 2 
                 20 
                 75 
               
               
                 3 
                 40 
                 10 
               
               
                 4 
                 30 
                 5 
               
               
                 5 
                 25 
                 2 
               
               
                   
               
            
           
         
       
     
     It was found that in first 2 hours only 20 percent of the drug was released following the administration of enteric coated tablet of alkaloid free purified Ashwagandha root extract. But after 2 hours 40 percent of the drug was released and results at 4 and 5 hours showed complete release of enteric coated tablet of alkaloid free purified Ashwagandha root extract. 
     Example 13 
     Efficacy Study of Different Ashwagandha Extracts. 
     44 rats were divided into eleven groups having 4 rats in each group. The animals were trained for swimming test (15 minutes) as pre-test session and then fasted overnight. To the fasted animals, reserpine (6 mg/kg) was injected i.p. to depress the animals. After 1 hour of reserpine injection, the test samples/standard was fed orally as designated dose. After 1 hour of test sample/standard, the rats were tested using forced swim test and duration of immobility in the 5 minutes test session was recorded. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Segregation of rats for experimental study 
               
            
           
           
               
               
            
               
                 Groups 
                 Treatment 
               
               
                   
               
               
                 Group I 
                 Normal control (Received only vehicle; 1% Tween 80 
               
               
                   
                 v/v p.o.). 
               
               
                 Group II 
                 Reserpine (6 mg/kg i.p) + Vehicle (1% Tween 80 v/v p.o.). 
               
               
                 Group III 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum  
               
               
                   
                 5% withanolides (Dosage 100 mg/kg p.o). 
               
               
                 Group IV 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum  
               
               
                   
                 2.5% withanolides (Dosage 100 mg/kg p.o). 
               
               
                 Group V 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum  
               
               
                   
                 1.8% withanolides and minimum 2.5% alkaloids  
               
               
                   
                 (Dosage 100 mg/kg p.o). 
               
               
                 Group VI 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum  
               
               
                   
                 1.1% withanolides (Dosage 100 mg/kg p.o). 
               
               
                 GroupVII 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum  
               
               
                   
                 0.7% withanolides (sample 2 prepared as per example 6)  
               
               
                   
                 (Dosage 100 mg/kg p.o). 
               
               
                 Group VIII 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum  
               
               
                   
                 20% withanolide glycosides (sample 2 prepared as per  
               
               
                   
                 example 3) (Dosage 100 mg/kg p.o). 
               
               
                 Group IX 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha extract with  
               
               
                   
                 35% withanolide glycosides (sample 3 prepared as per  
               
               
                   
                 example 4) (Dosage: 100 mg/kg p.o.). 
               
               
                 Group X 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha extract with  
               
               
                   
                 80% withanolide glycosides (sample 5 prepared as per  
               
               
                   
                 example 5) (Dosage: 100 mg/kg p.o.). 
               
               
                 Group XI 
                 Reserpine (6 mg/kg i.p) + Fluoxetine (10 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Effect of different Ashwagandha extracts on Immobility time in rats. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Immobility 
               
               
                   
                   
                 time in 
               
               
                 Groups 
                 Treatment 
                 seconds 
               
               
                   
               
               
                 Group I 
                 Normal control (Received only vehicle;  
                 120 
               
               
                   
                 1% Tween 80 v/v p.o.). 
                   
               
               
                 Group II 
                 Reserpine (6 mg/kg i.p) + Vehicle  
                 210 
               
               
                   
                 (1% Tween 80 v/v p.o.). 
                   
               
               
                 Group III 
                 Reserpine (6 mg/kg i.p) + Ashwagandha  
                 200 
               
               
                   
                 extract with minimum 
                   
               
               
                   
                 5% withanolides (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group IV 
                 Reserpine (6 mg/kg i.p) + Ashwagandha  
                 205 
               
               
                   
                 extract with minimum 
                   
               
               
                   
                 2.5% withanolides (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group V 
                 Reserpine (6 mg/kg i.p) + Ashwagandha  
                 200 
               
               
                   
                 extract with minimum 
                   
               
               
                   
                 1.8% withanolides and minimum 2.5% alkaloids  
                   
               
               
                   
                 (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group VI 
                 Reserpine (6 mg/kg i.p) + Ashwagandha  
                 195 
               
               
                   
                 extract with minimum 
                   
               
               
                   
                 1.1% withanolides (Dosage 100 mg/kg p.o). 
                   
               
               
                 GroupVII 
                 Reserpine (6 mg/kg i.p) + Ashwagandha 
                 190 
               
               
                   
                 extract with minimum 
                   
               
               
                   
                 0.7% withanolides (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group  
                 Reserpine (6 mg/kg i.p) + Ashwagandha  
                 180 
               
               
                 VIII 
                 extract with minimum 
                   
               
               
                   
                 20% withanolide glycosides (Dosage  
                   
               
               
                   
                 100 mg/kg p.o). 
                   
               
               
                 Group IX 
                 Reserpine(6 mg/kgi. p) + Purified  
                 145 
               
               
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides (Dosage:  
                   
               
               
                   
                 100 mg/kg p.o.). 
                   
               
               
                 Group X 
                 Reserpine(6 mg/kgi. p) + Purified  
                 135 
               
               
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                 withanolide glycosides (Dosage:  
                   
               
               
                   
                 100 mg/kg p.o.). 
                   
               
               
                 Group XI 
                 Reserpine(6 mg/kg i.p) + Fluoxetine  
                 122 
               
               
                   
                 (10 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     As shown in the Table 2, immobility time for normal control animals was 120 seconds. When reserpine was injected i.p., the animals got depressed and immobility time was increased to 210 seconds (Group II). Oral administration of Ashwagandha root extract with minimum 5% of withanolides (100 mg/kg) after reserpine injection was not much effective and immobility time was recorded as 200 seconds (Group III). Similarly, rats treated with Ashwagandha extract with minimum 2.5%, 1.8%, 1.1% and 0.7% of withanolides (100 mg/kg) were also not effective and immobility time was recorded near to 200 seconds (Group IV to Group VII). When animals were treated with Ashwagandha extract with 20%, 35% and 80% of withanolide glycosides (100 mg/kg), the immobility time was reduced significantly to 180, 145 and 135 seconds respectively (Group VIII to Group X). In case of fluoxetine standard at 10 mg/kg (Group XI), the immobility time was only 122 seconds which is almost similar to normal control animals. 
     Example 14 
     Effect of Different Enteric and Nonenteric Coated Ashwagandha Extracts on Immobility Time in Rats. 
     92 rats were divided into twenty three groups. Each group had 4 rats. The animals were trained for swimming test (15 minutes) as pre-test session and then fasted overnight. To the fasted animals, reserpine (6 mg/kg) was injected i.p. to depress the animals. After 1 hour of reserpine injection, the test samples/standard was fed orally as designated dose. After 1 hour of test sample/standard, the rats were tested using forced swim test and duration of immobility in the 5 minutes test session was recorded. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Segregation of rats for experimental study. 
               
            
           
           
               
               
            
               
                 Groups 
                 Treatment 
               
               
                   
               
               
                 Group I 
                 Normal control (Received only vehicle; 1% Tween 80 v/v p.o.). 
               
               
                 Group II 
                 Reserpine (6 mg/kg i.p) + Vehicle (1% Tween 80 v/v p.o.). 
               
               
                 Group III 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 6% withanolide 
               
               
                   
                 glycosides (sample 2 prepared as per example 2) (Dosage 100 mg/kg p.o). 
               
               
                 Group IV 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 6% withanolide 
               
               
                   
                 glycosides (sample 2 prepared as per example 2) (Dosage 20 mg/kg p.o). 
               
               
                 Group V 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 6% withanolide 
               
               
                   
                 glycosides (sample 2 prepared as per example 2) coated with pectin (prepared as 
               
               
                   
                 per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group VI 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with 6% minimum withanolide 
               
               
                   
                 glycosides (sample 2 prepared as per example 2) coated with Eudragit (prepared 
               
               
                   
                 as per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 1% withanolide 
               
               
                 VII 
                 glycoside coated with Eudragit (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 20% withanolide 
               
               
                 VIII 
                 glycoside (sample 2 prepared as per example 3) (Dosage 100 mg/kg p.o). 
               
               
                 Group IX 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 20% withanolide 
               
               
                   
                 glycoside (sample 2 prepared as per example 3) (Dosage 20 mg/kg p.o). 
               
               
                 Group X 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 20% withanolide 
               
               
                   
                 glycoside(sample 2 prepared as per example 3) coated with Eudragit (prepared as 
               
               
                   
                 per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group XI 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 35% 
               
               
                   
                 withanolide glycoside (sample 3 prepared as per example 4) (Dosage 100 mg/kg 
               
               
                   
                 p.o). 
               
               
                 Group XII 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 35% 
               
               
                   
                 withanolide glycoside (sample 3 prepared as per example 4) (Dosage 20 mg/kg 
               
               
                   
                 p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 35% 
               
               
                 XIII 
                 withanolide glycoside (sample 3 prepared as per example 4) coated with pectin 
               
               
                   
                 (prepared as per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 35% 
               
               
                 XIV 
                 withanolide glycoside (sample 3 prepared as per example 4) coated with Hydroxy 
               
               
                   
                 propyl methyl cellulose (prepared as per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 35% 
               
               
                 XV 
                 withanolide glycoside (sample 3 prepared as per example 4) coated with Poly 
               
               
                   
                 vinyl acetate (PVA) (prepared as per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 35% 
               
               
                 XVI 
                 withanolide glycoside (sample 3 prepared as per example 4) coated with Shellac 
               
               
                   
                 (prepared as per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 35% 
               
               
                 XVII 
                 withanolide glycoside (sample 3 prepared as per example 4) coated with 
               
               
                   
                 Cellulose acetate phthalate (CAP) (prepared as per example 8) (Dosage 20 mg/kg 
               
               
                   
                 p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 35% 
               
               
                 XVIII 
                 withanolide glycoside (sample 3 prepared as per example 4) coated with Eudragit 
               
               
                   
                 (prepared as per example 8). (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 80% 
               
               
                 XIX 
                 withanolide glycoside (sample 5 prepared as per example 5) (Dosage 100 mg/kg 
               
               
                   
                 p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 80% 
               
               
                 XX 
                 withanolide glycoside (sample 5 prepared as per example 5) (Dosage 20 mg/kg 
               
               
                   
                 p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 80% 
               
               
                 XXI 
                 withanolide glycoside (sample 5 prepared as per example 5) coated with pectin 
               
               
                   
                 (prepared as per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract with 80% 
               
               
                 XXII 
                 withanolide glycoside (sample 5 prepared as per example 5) coated with 
               
               
                   
                 Eudragit (prepared as per example 8). (Dosage 20 mg/kg p.o). 
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Fluoxetine (10 mg/kg p.o.). 
               
               
                 XXIII 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Effect of enteric and nonenteric coated purified Ashwagandha extracts on 
               
               
                 Immobility time in rats. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Immobility 
               
               
                   
                   
                 time in 
               
               
                 Groups 
                 Treatment 
                 seconds 
               
               
                   
               
            
           
           
               
               
               
            
               
                 Group I 
                 Normal control (Received only vehicle; 1% Tween 80 v/v p.o.).  
                 120 
               
               
                 Group II 
                 Reserpine (6 mg/kg i.p) + Vehicle (1% Tween 80 v/v p.o.). 
                 250 
               
               
                 Group III 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 
                 240 
               
               
                   
                 6% withanolide glycoside (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group IV 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 
                 248 
               
               
                   
                 6% withanolide glycoside (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group V 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 
                 245 
               
               
                   
                 6% withanolide glycoside coated with pectin (Dosage 20 mg/kg 
                   
               
               
                   
                 p.o). 
                   
               
               
                 Group VI 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 
                 195 
               
               
                   
                 6% withanolide glycoside coated with Eudragit (Dosage 
                 20 
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group VII 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 
                 242 
               
               
                   
                 1% withanolide glycoside coated with Eudragit (Dosage 
                 20 
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group VIII 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 
                 235 
               
               
                   
                 20% withanolide glycoside (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group IX 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 
                 239 
               
               
                   
                 20% withanolide glycoside (Dosage 20 mg/kg p.o)  
                   
               
               
                 Group X 
                 Reserpine (6 mg/kg i.p) + Ashwagandha extract with minimum 
                 190 
               
               
                   
                 20% withanolide glycoside coated with Eudragit (Dosage 
                 20 
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group XI 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha extract with 
                 175 
               
               
                   
                 35% withanolide glycoside (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group XII 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha extract with 
                 210 
               
               
                   
                 35% withanolide glycoside (Dosage 20 mg/kg p.o)  
                   
               
               
                 Group XIII 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha extract with 
                 205 
               
               
                   
                 35% withanolide glycoside coated with pectin (Dosage 
                 20 
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group XIV 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract 
                 207 
               
               
                   
                 with 35% withanolide glycoside coated with Hydroxy propyl 
                   
               
               
                   
                 methyl cellulose (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group XV 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract 
                 204 
               
               
                   
                 with 35% withanolide glycoside coated with Poly vinyl acetate 
                   
               
               
                   
                 (PVA) (Dosage 20 mg/kg p.o)  
                   
               
               
                 Group XVI 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract 
                 162 
               
               
                   
                 with 35% withanolide glycoside coated with Shellac (Dosage 20 
                   
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract 
                 164 
               
               
                 XVII 
                 with 35% withanolide glycoside coated with Cellulose acetate 
                   
               
               
                   
                 phthalate (CAP) (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha extract with  
                 160 
               
               
                 XVIII 
                 35% withanolide glycoside coated with Eudragit. (Dosage 20 
                   
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group XIX 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha extract with  
                 166 
               
               
                   
                 80% withanolide glycoside (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group XX 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha extract with 
                 192 
               
               
                   
                 80% withanolide glycoside (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group XXI 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract 
                 189 
               
               
                   
                 with 80% withanolide glycoside coated with pectin (Dosage 20 
                   
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Purified Ashwagandha root extract  
                 122 
               
               
                 XXII 
                 with 80% withanolide glycoside coated with Eudragit. (Dosage 
                   
               
               
                   
                 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) + Fluoxetine (10 mg/kg p.o.). 
                 122 
               
               
                 XXIII 
                   
                   
               
               
                   
               
            
           
         
       
     
     As shown in the Table 2, immobility time for normal control animals was 120 seconds. When reserpine was injected i.p., the animals got depressed and immobility time was increased to 250 seconds (Group II). Oral administration of Ashwagandha extract with 6% withanolide glycosides at 100 mg/kg and 20 mg/kg dosage and Ashwagandha extract with 6% withanolide glycosides coated with pectin after reserpine injection was not much effective and immobility time was recorded as above 240 seconds (Group III-V). But in group VI, Ashwagandha extract with 6% withanolide glycosides coated with Eudragit after reserpine injection, the immobility time was recorded as 195 seconds. When animals were treated with Ashwagandha extract containing minimum 1% withanolide glycosides coated with Eudragit at 20 mg/kg dose, it was not effective and immobility time was found as 242 seconds (Group VII). When animals were treated with Ashwagandha root extract containing minimum 20% withanolide glycosides at 100 mg/Kg dosage, immobility time was 235 seconds (Group VIII) and Ashwagandha root extract containing minimum 20% withanolide glycosides at 20 mg/Kg dosage, immobility time was 239 seconds (Group IX). Whereas Ashwagandha root extract with 20% withanolide glycosides coated with Eudragit at 20 mg/kg dose, immobility time was reduced to 190 seconds (Group X). The immobility time was found as 175 seconds and 210 seconds when animals were treated with purified Ashwagandha extract with 35% withanolide glycosides at 100 mg/kg dose (Group IX) and 20 mg/kg dose (Group XII) respectively. When animals were treated with purified Ashwagandha extract with 35% withanolide glycosides coated with pectin, HPMC and PVA at 20 mg/kg dose, the immobility time was 205, 207 and 204 seconds respectively which indicates very less effectiveness of such non-enteric coatings (Group XIII-XV). In spite of nonenteric coatings, when animals were treated with Ashwagandha extract with 35% withanolide glycosides coated with enteric materials shellac, CAP or Eudragit at 20 mg/kg dose, the immobility time was reduced to 162, 164 and 160 seconds respectively (Group XVI-XVIII) indicating the effectiveness of enteric coating. Animals treated with purified Ashwagandha extract with 80% withanolide glycoside at 100 mg/kg dose and 20 mg/Kg dose showed immobility time as 166 seconds (Group XIX) and 192 seconds (Group XX) respectively. When animals were treated with purified Ashwagandha extract with 80% withanolide glycoside coated with pectin, the immobility time was 189 seconds whereas when same extract was coated with Eudragit, the immobility time was reduced to 122 seconds only showing the effectiveness of enteric coating (Group XXI-XXII). In case of fluoxetine standard (Group XXIII), the immobility time was only 122 seconds which is almost similar to normal control animals. 
     Example 15 
     Bioavailability Study Using Different Enteric and Nonenteric Coated Ashwagandha Extracts. 
     NZ white rabbits weighing 2-2.5 Kg of both sexes were used for the study. The rabbits were kept individually in stainless steel cages and maintained in well ventilated room under normal and uniform conditions like 12 hours light and dark cycle and at 25±2° C. Water and feed were given ad-libitum. The animals were divided into twenty four groups. Each group had 3 animals. All the animals underwent fasting for 12 hours before administration of test samples. The animals were divided as shown in table 1. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Segregation of rabbits for experimental study. 
               
            
           
           
               
               
            
               
                 Groups 
                 Treatment 
               
               
                   
               
               
                 Group I 
                 Control (1% Tween 80) (Dose: 10 mL/Kg.body wt.). 
               
               
                 Group II 
                 Ashwagandha extract with minimum 6% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 2) (Dosage 100 mg/kg p.o). 
               
               
                 Group III 
                 Ashwagandha extract with minimum 6% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 2) (Dosage 20 mg/kg p.o). 
               
               
                 Group IV 
                 Ashwagandha extract with minimum 6% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 2) coated with pectin (prepared as per example 8)  
               
               
                   
                 (Dosage 20 mg/kg p.o). 
               
               
                 Group V 
                 Ashwagandha extract with minimum 6% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 2) coated with Eudragit (prepared as per example 8)  
               
               
                   
                 (Dosage 20 mg/kg p.o). 
               
               
                 Group VI 
                 Ashwagandha extract with minimum 1% withanolide glycoside coated with 
               
               
                   
                 Eudragit (Dosage 20 mg/kg p.o). 
               
               
                 Group VII 
                 Ashwagandha extract with minimum 20% withanolide glycoside(sample 2 
               
               
                   
                 prepared as per example 3) (Dosage 100 mg/kg p.o). 
               
               
                 Group VIII 
                 Ashwagandha extract with minimum 20% withanolide glycoside(sample 2 
               
               
                   
                 prepared as per example 3) (Dosage 20 mg/kg p.o). 
               
               
                 Group IX 
                 Ashwagandha extract with minimum 20% withanolide glycoside (sample 2 
               
               
                   
                 prepared as per example 3) coated with Eudragit (prepared as per example 8)  
               
               
                   
                 (Dosage 20 mg/kg p.o). 
               
               
                 Group X 
                 Purified Ashwagandha extract with 35% withanolide glycoside (sample 3 
               
               
                   
                 prepared as per example 4) (Dosage 100 mg/kg p.o). 
               
               
                 Group XI 
                 Purified Ashwagandha extract with 35% withanolide glycoside (sample 3 
               
               
                   
                 prepared as per example 4) (Dosage 20 mg/kg p.o). 
               
               
                 Group XII 
                 Purified Ashwagandha extract with 35% withanolide glycoside (sample 3 
               
               
                   
                 prepared as per example 4) coated with pectin (prepared as per example 8)  
               
               
                   
                 (Dosage 20 mg/kg p.o). 
               
               
                 Group XIII 
                 Purified Ashwagandha root extract with 35% (sample 3 prepared as per 
               
               
                   
                 example 4) coated with Hydroxy propyl methyl cellulose (prepared as per 
               
               
                   
                 example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group XIV 
                 Purified Ashwagandha root extract with 35% withanolide glycoside (sample 3 
               
               
                   
                 prepared as per example 4) coated with Poly vinyl acetate (PVA) (prepared as 
               
               
                   
                 per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group XV 
                 Purified Ashwagandha root extract with 35% withanolide glycoside (sample 3 
               
               
                   
                 prepared as per example 4) coated with Shellac (prepared as per example 8)  
               
               
                   
                 (Dosage 20 mg/kg p.o). 
               
               
                 Group XVI 
                 Purified Ashwagandha root extract with 35% withanolide glycoside (sample 3 
               
               
                   
                 prepared as per example 4) coated with Cellulose acetate phthalate (CAP)  
               
               
                   
                 (prepared as per example 8) (Dosage 20 mg/kg p.o). 
               
               
                 Group XVII 
                 Purified Ashwagandha extract with 35% withanolide glycoside (sample 3 
               
               
                   
                 prepared as per example 4) coated with Eudragit (prepared as per example 8). 
               
               
                   
                 (Dosage 20 mg/kg p.o)  
               
               
                 Group XVIII 
                 Purified Ashwagandha extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) (Dosage 100 mg/kg p.o). 
               
               
                 Group XIX 
                 Purified Ashwagandha extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) (Dosage 20 mg/kg p.o). 
               
               
                 Group XX 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) coated with pectin (prepared as per example 8)  
               
               
                   
                 (Dosage 20 mg/kg p.o). 
               
               
                 Group XXI 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) coated with Eudragit (prepared as per example 8)  
               
               
                   
                 (Dosage 20 mg/kg p.o). 
               
               
                 Group XXII 
                 Ashwagandha extract with 3.5% withanolide glycosides (sample 2 prepared 
               
               
                   
                 as per example 19) (Dosage 100 mg/kg p.o)  
               
               
                 Group XXIII 
                 Ashwagandha extract with 3.5% withanolide glycosides (sample 2 prepared 
               
               
                   
                 as per example 19) (Dosage 20 mg/kg p.o)  
               
               
                 Group XXIV 
                 Ashwagandha extract with 3.5% withanolide glycosides (sample 2 prepared 
               
               
                   
                 as per example 19) coated with Eudragit (prepared as per example 20)  
               
               
                   
                 (Dosage 20 mg/kg p.o) 
               
               
                   
               
            
           
         
       
     
     The animals were able to access drinking water freely. The extracts were prepared in 1% Tween 80 and fed orally. After 2 hr 30 minutes, 2 ml blood was collected from each rabbit via marginal ear vein in EDTA coated vacutainer tubes. The blood samples were centrifuged at 3000 rpm for 15 minutes and plasma was separated carefully. The plasma samples were stored at −80° C. until analysis. The concentration of withanolide glycosides was analyzed by HPLC. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Bioavailability study using different enteric and nonenteric coated Ashwagandha extracts. 
               
            
           
           
               
               
               
            
               
                   
                   
                 withanolide 
               
               
                   
                   
                 glycosides 
               
               
                 Groups 
                 Treatment 
                 (ng/ml) 
               
               
                   
               
               
                 Group I 
                 Control (Received only vehicle; 1% Tween 80 v/v p.o.). 
                 ND 
               
               
                 Group II 
                 Ashwagandha extract with minimum 6% withanolide 
                 ND 
               
               
                   
                 glycosides (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group III 
                 Ashwagandha extract with minimum 6% withanolide 
                 ND 
               
               
                   
                 glycosides (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group IV 
                 Ashwagandha extract with minimum 6% withanolide 
                 ND 
               
               
                   
                 glycosides coated with Pectin (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group V 
                 Ashwagandha extract with minimum 6% withanolide 
                 15.2 
               
               
                   
                 glycosides coated with Eudragit (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group VI 
                 Ashwagandha extract with minimum 1% withanolide 
                 1.6 
               
               
                   
                 glycoside coated with Eudragit (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group VII 
                 Ashwagandha extract with minimum 20% withanolide 
                 1.9 
               
               
                   
                 glycoside (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group 
                 Ashwagandha extract with minimum 20% withanolide 
                 0.3 
               
               
                 VIII 
                 glycoside (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group IX 
                 Ashwagandha extract with minimum 20% withanolide 
                 4.2 
               
               
                   
                 glycoside coated with Eudragit (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group X 
                 Purified Ashwagandha extract with 35% withanolide 
                 60.3 
               
               
                   
                 glycoside (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group XI 
                 Purified Ashwagandha extract with 35% withanolide 
                 12.4 
               
               
                   
                 glycoside (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group XII 
                 Purified Ashwagandha extract with 35% withanolide 
                 13.8 
               
               
                   
                 glycoside coated with pectin (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Purified Ashwagandha root extract with 35% withanolide  
                 12.9 
               
               
                 XIII 
                 glycoside coated with Hydroxy propyl methyl cellulose 
                   
               
               
                   
                 (Dosage20 mg/kg p.o). 
                   
               
               
                 Group 
                 Purified Ashwagandha root extract with 35% withanolide 
                 13.2 
               
               
                 XIV 
                 glycoside coated with Poly vinyl acetate (PVA) (Dosage 20 
                   
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group XV 
                 Purified Ashwagandha root extract with 35% withanolide 
                 100.3 
               
               
                   
                 glycoside coated with Shellac (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Purified Ashwagandha root extract with 35% withanolide 
                 101.9 
               
               
                 XVI 
                 glycoside coated with Cellulose acetate phthalate (CAP)  
                   
               
               
                   
                 (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Purified Ashwagandha extract with 35% withanolide 
                 102.3 
               
               
                 XVII 
                 glycoside coated with Eudragit. (Dosage 20 mg/kg p.o)  
                   
               
               
                 Group 
                 Purified Ashwagandha extract with 80% withanolide 
                 85.2 
               
               
                 XVIII 
                 glycoside (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group 
                 Purified Ashwagandha extract with 80% withanolide 
                 18.5 
               
               
                 XIX 
                 glycoside (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group XX 
                 Purified Ashwagandha root extract with 80% withanolide 
                 17.3 
               
               
                   
                 glycoside coated with pectin (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Purified Ashwagandha root extract with 80% withanolide 
                 149.8 
               
               
                 XXI 
                 glycoside coated with Eudragit (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Ashwagandha extract with 3.5% withanolide glycosides 
                 ND 
               
               
                 XXII 
                 (Dosage 100 mg/kg p.o). 
                   
               
               
                 Group 
                 Ashwagandha extract with 3.5% withanolide glycosides 
                 ND 
               
               
                 XXIII 
                 (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Purified Ashwagandha root extract with 3.5% withanolide  
                 10.2 
               
               
                 XXIV 
                 glycoside coated with Eudragit (Dosage 20 mg/kg p.o). 
                   
               
               
                   
               
            
           
         
       
     
     As shown in Table 2, withanolide glycosides were not detected in the plasma of animals fed with vehicle, Ashwagandha extract with minimum 6% withanolide glycosides or Ashwagandha root extract with minimum 6% withanolide glycosides coated with pectin (Group I-IV). When animals were fed with Ashwagandha root extract with minimum 6% withanolide glycosides coated with Eudragit at 20 mg/kg, the withanolide glycoside level was found as 15.2 ng/ml (Group V). Withanolide glycosides were not detected in the plasma of animals fed with Ashwagandha extract with 3.5% withanolide glycoside at 100 and 20 mg/kg dose (Group XXII-XXIII), whereas Ashwagandha root extract with 3.5% withanolide glycosides coated with Eudragit at 20 mg/kg, the withanolide glycoside level was found as 10.2 ng/ml (Group XXIV). Animals fed with Ashwagandha extract with minimum 20% withanolide glycosides at 100 and 20 mg/Kg showed withnolide glycoside level in plasma as 1.9 and 0.3 ng/ml respectively. Animals fed with Ashwagandha extract with minimum 1%, and 20% withanolide glycoside coated with Eudragit at 20 mg/kg showed withnolide glycoside level in plasma as 1.6 and 4.2 ng/ml showing the effectiveness of enteric coating in preserving the withanolide glycosides from hydrolysis in the stomach (Group VI &amp;IX). When animals were fed with purified Ashwagandha extract with 35% withanolide glycoside at 100 and 20 mg/kg dose, the withanolide glycoside level in plasma was found as 60.3 and 12.3 ng/ml (Group X-XI). Animals fed with purified Ashwagandha extract with 35% withanolide glycoside coated with pectin, HPMC or PVA at 20 mg/kg dose showed plasma level of withanolide glycosides as 13.8, 12.9 and 13.2 ng/ml respectively (Group XII-XIV). When the same extract, i.e. purified Ashwagandha extract with 35% withanolide glycoside was coated with enteric materials shellac, CAP or Eudragit, the plasma level of withanolide glycosides was increased to 100.3, 101.9 and 102.3 ng/ml respectively, indicating the effectiveness of enteric coating (XV-XVII). When animals were fed with purified Ashwagandha extract with 80% withanolide glycoside at 100 and 20 mg/kg dose, the plasma withanolide glycoside level was found as 85.2 and 18.5 ng/ml respectively (Group XVIII-XIX). When the same extract (purified Ashwagandha extract with 80% withanolide glycoside) was coated with pectin and fed at only 20 mg/kg dose, the withanolide glycoside level was found as 17.3 ng/ml only showing the noneffectiveness of nonenteric coating (Group XX). Whereas when animals were fed with purified Ashwagandha extract with 80% withanolide glycoside coated with eudragit at 20 mg/kg dose, the plasma withanolide glycoside level was found as 149.8 ng/ml showing the protective nature of enteric coating (Group XXI). 
     Example 16 
     Efficacy Studies of Ashwagandha Extracts 
     1. Antistress Activity 
     a. Anoxia Stress Tolerance Test 
     Albino Wistar rats of either sex weighing 150-220 g were selected and divided into 11 groups of six each and following treatment was given after one week of acclimatization: 
     
       
         
           
               
             
               
                 TABLE 1 
               
               
                   
               
               
                 Segregation of rats for experimental study 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Group I 
                 Control (Received only vehicle; 1% Tween 80 v/v p.o.)  
               
               
                 Group II 
                 Ashwagandha root extract with 5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 6) (100 mg/kg p.o.). 
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 6) coated with Eudragit (prepared as per example 
               
               
                   
                 8) (100 mg/kg p.o.). 
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root extract (sample 2 prepared as 
               
               
                   
                 per example 1) (50 mg/kg p.o.). 
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) (50 mg/kg p.o.). 
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) coated with Eudragit(prepared as per example 8)  
               
               
                   
                 (50 mg/kg p.o.). 
               
               
                 Group VII 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 19) (50 mg/kg p.o.). 
               
               
                 Group VIII 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 19) coated with Eudragit (prepared as per example 
               
               
                   
                 20) (50 mg/kg p.o.). 
               
               
                 Group IX 
                 Ashwagandha raw powder prepared as per example 24(100 mg/kg p.o.). 
               
               
                 Group X 
                 Ashwagandha raw powder (prepared as per example 24) coated with Eudragit 
               
               
                   
                 (100 mg/kg p.o.). 
               
               
                 Group XI 
                 Diazepam (2 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     Animals were treated as shown above for the 3 weeks. At the end of 1st, 2nd and 3rd week i.e. on 7th, 14th and 21st day 1 h after the treatment stress was induced by placing each animal individually in the hermetic vessel of 1 L capacity to record anoxia tolerance time. The time duration of entry of the animal into the hermetic vessel and the appearance of the first convulsion was taken as time of anoxia. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha extracts on anoxia stress tolerance time in rats. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Duration of anoxia stress 
               
               
                   
                   
                 tolerance in minutes 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                 First 
                 Second 
                 Third 
               
               
                 Groups 
                 Treatment 
                 week 
                 week 
                 week 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 Group I 
                 Control (1% Tween 80 v/v p.o.). 
                 18.50 
                 18.75 
                 19.25 
               
               
                 Group II 
                 Ashwagandha root extract with 5% 
                 21.33 
                 27.67 
                 29.00 
               
               
                   
                 withanolide glycosides (100 mg/kg p.o). 
                   
                   
                   
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide 
                 22.67 
                 29.67 
                 30.33 
               
               
                   
                 glycosides coated with Eudragit (100 mg/kg 
                   
                   
                   
               
               
                   
                 p.o). 
                   
                   
                   
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root 
                 19.33 
                 21.67 
                 21.00 
               
               
                   
                 extract (50 mg/kg p.o.). 
                   
                   
                   
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% 
                 27.50 
                 30.33 
                 32.67 
               
               
                   
                 withanolide glycosides (50 mg/kg p.o.). 
                   
                   
                   
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% 
                 31.67 
                 39.33 
                 48.67 
               
               
                   
                 withanolide glycosides coated with Eudragit 
                   
                   
                   
               
               
                   
                 (50 mg/kg p.o.). 
                   
                   
                   
               
               
                 Group VII 
                 Ashwagandha root extract with 3.5% 
                 24.96 
                 26.47 
                 27.12 
               
               
                   
                 withanolide glycosides (50 mg/kg p.o.). 
                   
                   
                   
               
               
                 Group 
                 Ashwagandha root extract with 3.5% 
                 27.63 
                 32.78 
                 38.98 
               
               
                 VIII 
                 withanolide glycosides coated with Eudragit 
                   
                   
                   
               
               
                   
                 (50 mg/kg p.o.). 
                   
                   
                   
               
               
                 Group IX 
                 Ashwagandha raw powder (100 mg/kg p.o.). 
                 20.43 
                 23.14 
                 24.84 
               
               
                 Group X 
                 Ashwagandha raw powder coated with 
                 21.83 
                 23.79 
                 25.13 
               
               
                   
                 Eudragit (100 mg/kg p.o.). 
                   
                   
                   
               
               
                 Group XI 
                 Diazepam (2 mg/kg p.o.). 
                 34.50 
                 37.33 
                 37.00 
               
               
                   
               
            
           
         
       
     
     The results obtained from the anoxia stress tolerance test were expressed as average of six rats (Table 2). Anoxia stress tolerance time was significantly enhanced on 7th, 14th and 21st day in Purified Ashwagandha root extract with 80% withanolide glycosides (50 mg/kg), Purified Ashwagandha root extract with 80% withanolide glycosides coated with Eudragit (50 mg/kg) and Diazepam (2 mg/kg) treated groups. Anoxia stress tolerance time was enhanced on 7th, 14th and 21st day in Ashwagandha root extract with 3.5% withanolide glycosides (50 mg/kg), Ashwagandha root extract with 3.5% withanolide glycosides coated with Eudragit (50 mg/kg). There was increased anoxia tolerance time also seen after 2nd and 3rd week of Ashwagandha root extract with 5% withanolide glycosides (50 mg/kg) treated group but not statistically significant result was obtained on 7th day. Ashwagandha raw powder and Ashwagandha raw powder with enteric coating showed slight increase in anoxia tolerance time (100 mg/Kg). However the effect of alkaloidal rich fraction of Ashwagandha root extract (50 mg/kg) on tolerance time was not statistically significant at the end of 1st, 2nd and 3rd week of treatment. 
     b. Swimming Endurance Test 
     Swiss mice of either sex weighing 20-25 g were selected and divided into nine groups of six each and following treatment was given after one week of acclimatization: 
     
       
         
           
               
             
               
                 TABLE 3 
               
               
                   
               
               
                 Segregation of mice for experimental study. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Group I 
                 Control (Received only vehicle; 1% Tween 80 v/v p.o.). 
               
               
                 Group II 
                 Ashwagandha root extract with 5% withanolide glycosides (sample 2 prepared as 
               
               
                   
                 per example 6) (100 mg/kg p.o.). 
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide glycosides coated with Eudragit 
               
               
                   
                 (prepared as per example 8) (100 mg/kg p.o.). 
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root extract (sample 2 prepared as per 
               
               
                   
                 example 1) (50 mg/kg p.o.). 
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) (50 mg/kg p.o.). 
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) coated with Eudragit(prepared as per example 8) (50 
               
               
                   
                 mg/kg p.o.). 
               
               
                 Group VII 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 prepared 
               
               
                   
                 as per example 19) (50 mg/kg p.o.). 
               
               
                 Group 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 prepared 
               
               
                 VIII 
                 as per example 19) coated with Eudragit (prepared as per example 20) (50 
               
               
                   
                 mg/kg p.o.). 
               
               
                 Group IX 
                 Diazepam (2 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     Extracts were given to the mice, once daily for period of 7 days. On 8th day the mice were subjected to swimming stress by keeping them in propylene tank of dimension (37×37×30 cm), filled with water to a height of 25 cm. The rats were allowed to swim till complete exhaustion and the endpoint was taken when the animal started drowning. The mean swimming time for each group was calculated. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha extracts on swimming endurance in mice. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Swimming time 
               
               
                 Groups 
                 Treatment 
                 in minutes 
               
               
                   
               
               
                 Group I 
                 Control (1% Tween 80 v/v p.o.). 
                 32.50 
               
               
                 Group II 
                 Ashwagandha root extract with 5% withanolide 
                 39.67 
               
               
                   
                 glycosides (100 mg/kg p.o.). 
                   
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide 
                 40.33 
               
               
                   
                 glycosides coated with Eudragit (100 mg/kg p.o.). 
                   
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root extract (50 
                 33.75 
               
               
                   
                 mg/kg p.o.). 
                   
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% withanolide 
                 42.00 
               
               
                   
                 glycoside (50 mg/kg p.o.). 
                   
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% withanolide 
                 45.50 
               
               
                   
                 glycoside coated with Eudragit (50 mg/kg p.o.). 
                   
               
               
                 Group VII 
                 Ashwagandha root extract with 3.5% withanolide 
                 40.72 
               
               
                   
                 glycosides (50 mg/kg p.o.). 
                   
               
               
                 Group 
                 Ashwagandha root extract with 3.5% withanolide 
                 42.81 
               
               
                 VIII 
                 glycosides coated with Eudragit (50 mg/kg p.o.). 
                   
               
               
                 Group VII 
                 Diazepam (2 mg/kg p.o.). 
                 58.67 
               
               
                   
               
            
           
         
       
     
     The swimming endurance time was significantly enhanced on 8th day in purified Ashwagandha root extract with 80% withanolide glycoside (50 mg/kg), purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit (50 mg/kg) and Diazepam (2 mg/kg) treated groups as compared to the control group. The swimming time was also enhanced in Ashwagandha root extract with 5% withanolide glycosides (100 mg/kg) group but it was less than purified Ashwagandha root extract with 80% withanolide glycoside or purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit groups. The swimming endurance time was enhanced in Ashwagandha root extract with 3.5% withanolide glycoside (50 mg/kg) and Ashwagandha root extract with 3.5% withanolide glycoside coated with Eudragit (50 mg/kg) as compared to the control group. Alkaloidal rich fraction of Ashwagandha root extract had very little effect on increasing the swimming time. 
     2. Immunomodulatory Activity 
     a. Effect of  Withania somnifera  Extracts on the Bone Marrow Cellularity and α-Esterase Positive Cells. 
     Forty two Balb/c mice (20-25 gm) were divided into eight groups with 6 mice in each group. The following treatment was given for 7 days: 
     
       
         
           
               
             
               
                 TABLE 5 
               
               
                   
               
               
                 Segregation of mice for experimental study. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Group I 
                 Control (Received only vehicle; 1% Tween 80 v/v p.o.)  
               
               
                 Group II 
                 Ashwagandha root extract with 5% withanolide glycosides (sample 2 prepared 
               
               
                   
                 as per example 6) (100 mg/kg p.o.). 
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide glycosides coated with 
               
               
                   
                 Eudragit (prepared as per example 8) (100 mg/kg p.o.). 
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root extract (sample 2 prepared as 
               
               
                   
                 per example 1) (50 mg/kg p.o.). 
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) (50 mg/kg p.o.). 
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) coated with Eudragit(prepared as per example 5)  
               
               
                   
                 (50 mg/kg p.o.). 
               
               
                 Group VII 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 19) (50 mg/kg p.o.). 
               
               
                 Group VIII 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 19) coated with Eudragit (prepared as per example 
               
               
                   
                 20) (50 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     Animals were sacrificed after the last dose of drug treatment and bone marrow was collected from femur into medium containing 2% Fetal Calf Serum. The number of bone marrow cells was determined using a haemocytometer and expressed as total live cells/femur. A smear of the bone marrow cells from the above preparation was made on clean glass slides and stained with Para-rosaniline hydrochloride and counter stained with hematoxylin to determine the non-specific α-esterase positive cells. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha extracts on bone marrow cellularity and α-esterase 
               
               
                 activity. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 α-esterase 
               
               
                   
                   
                 Bone 
                 activity 
               
               
                   
                   
                 marrow 
                 (No. of α- 
               
               
                   
                   
                 cellularity 
                 esterase 
               
               
                   
                   
                 (cells/ 
                 positive 
               
               
                 Groups 
                 Treatment 
                 femur)  
                 cells) 
               
               
                   
               
               
                 Group I 
                 Control (1% Tween 80 v/v p.o.). 
                 14 × 10 6   
                 1030 
               
               
                 Group II 
                 Ashwagandha root extract with 5% withanolide 
                 19 × 10 6   
                 1410 
               
               
                   
                 glycosides (100 mg/kg p.o.). 
                   
                   
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide 
                 20 × 10 6   
                 1450 
               
               
                   
                 glycosides coated with Eudragit (100 mg/kg 
                   
                   
               
               
                   
                 P.O.). 
                   
                   
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root 
                 15 × 10 6   
                 1100 
               
               
                   
                 extract (50 mg/kg p.o.). 
                   
                   
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% 
                 24 × 10 6   
                 1740 
               
               
                   
                 withanolide glycoside (50 mg/kg p.o.). 
                   
                   
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% 
                 30 × 10 6   
                 2300 
               
               
                   
                 withanolide glycoside coated with Eudragit (50 
                   
                   
               
               
                   
                 mg/kg p.o.). 
                   
                   
               
               
                 Group VII 
                 Ashwagandha root extract with 3.5% 
                 21 × 10 6   
                 1575 
               
               
                   
                 withanolide glycosides (50 mg/kg p.o.). 
                   
                   
               
               
                 Group 
                 Ashwagandha root extract with 3.5% 
                 23 × 10 6   
                 1840 
               
               
                 VIII 
                 withanolide glycosides coated with Eudragit 
                   
                   
               
               
                   
                 (50 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     The effect of Ashwagandha extracts administration on the bone marrow cellularity and α-esterase positive cells is given in Table 6. Both the purified Ashwagandha root extract with 80% withanolide glycoside and purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit treated groups showed a significant increase in the bone marrow cells compared to control animals. Ashwagandha extract with 3.5% withanolide glycoside and Ashwagandha extract with 3.5% withanolide glycoside coated with Eudragit showed increase in the bone marrow cells compared to control animals. The Ashwagandha root extract with 5% withanolide glycosides treated animals also showed increase in the bone marrow cells, but it was less as compared to purified Ashwagandha root extract with 80% withanolide glycoside and purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit treated groups. There was very slight increase in bone marrow cells in the animals treated with alkaloidal rich fraction of Ashwagandha root extract. Moreover, the number of α-esterase positive cells were also found to be increased significantly in the purified Ashwagandha root extract with 80% withanolide glycoside and purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit treated groups compared to controls. Ashwagandha extract with 3.5% withanolide glycoside with and without coating showed increase in number of α-esterase positive cells compared to controls. 
     b. Effect of Ashwagandha Extracts on Circulating Antibody Titre. 
     Thirty Six Balb/c mice were immunized with SRBC (0.1 ml, i.p.) and divided into eight groups and treated as follows for 10 days: 
     
       
         
           
               
             
               
                 TABLE 7 
               
               
                   
               
               
                 Segregation of mice for experimental study. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Group I 
                 Control (Received only vehicle; 1% Tween 80 v/v p.o.). 
               
               
                 Group II 
                 Ashwagandha root extract with 5% withanolide glycosides (sample 2 prepared 
               
               
                   
                 as per example 6) (100 mg/kg p.o.). 
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide glycosides (sample 2 prepared 
               
               
                   
                 as per example 6) coated with Eudragit (prepared as per example 8) (100 mg/kg 
               
               
                   
                 P.O.). 
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root extract (sample 2 prepared as per 
               
               
                   
                 example 1) (50 mg/kg p.o.). 
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 5 
               
               
                   
                 prepared as per example 5) (50 mg/kg p.o.). 
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample5 
               
               
                   
                 prepared as per example 5) coated with Eudragit(prepared as per example 8) (50 
               
               
                   
                 mg/kg p.o.). 
               
               
                 Group 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 prepared 
               
               
                 VII 
                 as per example 19) (50 mg/kg p.o.). 
               
               
                 Group 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 prepared 
               
               
                 VIII 
                 as per example 19) coated with Eudragit (prepared as per example 20) (50 
               
               
                   
                 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     Blood was collected from the caudal vein prior to the antigen administration and after the last dose of extracts. Serum was separated and heat inactivated at 56° C. for 30 min. Antibody titre was estimated using SRBC as the antigen. For this, serum was serially diluted with PBS in 96 well round bottom titre plates. Equal volumes of 1% trypsnised SRBC (Ag) were added, mixed gently and incubated at room temperature for 3 h, and the agglutination titers were recorded. 
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha extracts on circulating antibody titre. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Antibody titre 
               
            
           
           
               
               
               
               
            
               
                 Groups 
                 Treatment 
                 Initial 
                 Final 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 Group I 
                 Control (1% Tween 80 v/v p.o.). 
                 22 
                 45 
               
               
                 Group II 
                 Ashwagandha root extract with 5% withanolide 
                 25 
                 242 
               
               
                   
                 glycosides (100 mg/kg p.o.). 
                   
                   
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide 
                 23 
                 253 
               
               
                   
                 glycosides coated with Eudragit (100 mg/kg p.o.). 
                   
                   
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root extract 
                 21 
                 98 
               
               
                   
                 (50 mg/kg p.o.). 
                   
                   
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% 
                 28 
                 480 
               
               
                   
                 withanolide glycoside (50 mg/kg p.o.). 
                   
                   
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% 
                 23 
                 750 
               
               
                   
                 withanolide glycoside coated with Eudragit (50 mg/kg 
                   
                   
               
               
                   
                 p.o.). 
                   
                   
               
               
                 Group VI 
                 Ashwagandha root extract with 3.5% withanolide 
                 26 
                 360 
               
               
                   
                 glycosides (50 mg/kg p.o.). 
                   
                   
               
               
                 Group VI 
                 Ashwagandha root extract with 3.5% withanolide 
                 24 
                 550 
               
               
                   
                 glycosides coated with Eudragit (50 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     The effect of  Withania somnifera  extracts administration on antibody titre is given in Table 8. Maximum antibody titre value of 750 was observed with purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit. Ashwagandha root extract with 3.5% withanolide glycoside coated with Eudragit showed the antibody titre value of 550. Maximum titre value from control animals was found to be 45 only. All other extracts also increased the titre value but these were less than the animals treated with purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit. 
     c. Effect of Ashwagandha Extracts on the Antibody Producing Cells. 
     The number of plaque forming cells from the spleen was determined by Plaque assay. Thirty six Balb/c mice were divided into eight groups and treated as follows for 7 days: 
     
       
         
           
               
             
               
                 TABLE 9 
               
               
                   
               
               
                 Segregation of mice for experimental study. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Group I 
                 Control (Received only vehicle; 1% Tween 80 v/v p.o.). 
               
               
                 Group II 
                 Ashwagandha root extract with 5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 6) (100 mg/kg p.o.). 
               
               
                 Group III 
                 Ashwagandha root extract with 5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 6) coated with Eudragit (prepared as per example 
               
               
                   
                 8) (100 mg/kg p.o.). 
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha root extract (sample 2 prepared as 
               
               
                   
                 per example 1) (50 mg/kg p.o.). 
               
               
                 Group V 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 
               
               
                   
                 5 prepared as per example 5) (50 mg/kg p.o.). 
               
               
                 Group VI 
                 Purified Ashwagandha root extract with 80% withanolide glycoside (sample 
               
               
                   
                 5 prepared as per example 5) coated with Eudragit(prepared as per example 
               
               
                   
                 8) (50 mg/kg p.o.). 
               
               
                 Group VII 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 19) (50 mg/kg p.o.). 
               
               
                 Group VIII 
                 Ashwagandha root extract with 3.5% withanolide glycosides (sample 2 
               
               
                   
                 prepared as per example 19) coated with Eudragit (prepared as per 
               
               
                   
                 example 20) (50 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     On 7 th  day the mice were immunized with SRBC (2.5×10 8 ). The animals were sacrificed after 4 days; spleens were processed into a single cell suspension and the cell number was adjusted to 8×10 6  cells/ml. Fifty microlitres of spleen cell suspension and 50 ml of Ag (SRBC 7%) were mixed with 0.5 ml of 0.5% molten agarose kept at 45° C. and spread on slides. After solidifying the agarose, the gels were incubated in the presence of compliment (rabbit serum at 37° C. for 1 h). Number of plaques was counted using a colony counter. 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha extracts on antibody producing cells. 
               
            
           
           
               
               
               
            
               
                   
                   
                 No. of  
               
               
                   
                   
                 PFC/10 6   
               
               
                 Groups 
                 Treatment 
                 spleen cells 
               
               
                   
               
            
           
           
               
               
               
            
               
                 Group I 
                 Control (1% Tween 80 v/v p.o.). 
                 300 
               
               
                 Group II 
                 Ashwagandha root extract with  
                 540 
               
               
                   
                 5% withanolide glycosides 
                   
               
               
                   
                 (100 mg/kg p.o.). 
                   
               
               
                 Group III 
                 Ashwagandha root extract  
                 560 
               
               
                   
                 with 5% withanolide glycosides 
                   
               
               
                   
                 coated with Eudragit (100 mg/kg p.o.). 
                   
               
               
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha  
                 350 
               
               
                   
                 root extract (50 mg/kg 
                   
               
               
                   
                 p.o.). 
                   
               
               
                 Group V 
                 Purified Ashwagandha root  
                 820 
               
               
                   
                 extract with 80% withanolide 
                   
               
               
                   
                 glycoside ( 50 mg/kg p.o.). 
                   
               
               
                 Group VI 
                 Purified Ashwagandha root  
                 1500 
               
               
                   
                 extract with 80% withanolide 
                   
               
               
                   
                 glycoside coated with Eudragit (50 mg/kg p.o.). 
                   
               
               
                 Group 
                 Ashwagandha root extract with  
                 620 
               
               
                 VII 
                 3.5% withanolide glycosides 
                   
               
               
                   
                 (50 mg/kg p.o.). 
                   
               
               
                 Group 
                 Ashwagandha root extract with  
                 890 
               
               
                 VIII 
                 3.5% withanolide glycosides 
                   
               
               
                   
                 coated with Eudragit (50 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     Ashwagandha extract administration was found to significantly enhance the number of antibody producing cells in spleen. The maximum number of plaque forming cells (PFC) was found in the animals treated with purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit. Ashwagandha root extract with 3.5% withanolide glycosides also showed an increased number of plaque forming cells (PFC). All other extracts also increased the number of plaque forming cells but these were less than the animals treated with purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit. 
     3. Anti-Inflammatory Activity. 
     Anti-inflammatory activity of Ashwagandha extracts was assessed by carrageenan induced paw edema model in rats. Forty five wistar rats were divided into nine groups (n=5). Following treatment was given to the rats as single dose: 
     
       
         
           
               
             
               
                 TABLE 11 
               
               
                   
               
               
                 Segregation of rats for experimental study. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Group I 
                 Control (1% Tween 80 v/v p.o.) 
               
               
                   
                 Group II 
                 Ashwagandha root extract with 5%  
               
               
                   
                   
                 withanolide glycosides (sample 2 
               
               
                   
                   
                 prepared as per example 6) (50 mg/kg p.o.). 
               
               
                   
                 Group III 
                 Ashwagandha root extract with 5%  
               
               
                   
                   
                 withanolide glycosides (sample 2 
               
               
                   
                   
                 prepared as per example 6) coated with  
               
               
                   
                   
                 Eudragit (prepared as per example 
               
               
                   
                   
                 8) (50 mg/kg p.o.). 
               
               
                   
                 Group IV 
                 Alkaloidal rich fraction of Ashwagandha  
               
               
                   
                   
                 root extract (sample 2 prepared as 
               
               
                   
                   
                 per example 1) (50 mg/kg p.o.). 
               
               
                   
                 Group V 
                 Purified Ashwagandha root extract  
               
               
                   
                   
                 with 80% withanolide glycoside (sample 
               
               
                   
                   
                 5 prepared as per example 5) ( 50 mg/kg p.o.). 
               
               
                   
                 Group VI 
                 Purified Ashwagandha root extract with  
               
               
                   
                   
                 80% withanolide glycoside (sample 
               
               
                   
                   
                 5 prepared as per example 5) coated  
               
               
                   
                   
                 with Eudragit(prepared as per example 
               
               
                   
                   
                 8) (50 mg/kg p.o.). 
               
               
                   
                 Group VII 
                 Ashwagandha root extract with 3.5%  
               
               
                   
                   
                 withanolide glycosides (sample 2 
               
               
                   
                   
                 prepared as per example 19) (50 mg/kg p.o.). 
               
               
                   
                 Group VIII 
                 Ashwagandha root extract with 3.5%  
               
               
                   
                   
                 withanolide glycosides (sample 2 
               
               
                   
                   
                 prepared as per example 19) coated  
               
               
                   
                   
                 with Eudragit (prepared as per example 
               
               
                   
                   
                 20) (50 mg/kg p.o.). 
               
               
                   
                 Group IX 
                 Indomethacin (10 mg/kg p.o.). 
               
               
                   
                   
               
            
           
         
       
     
     After 30 minutes of extracts 0.1 mL of carrageenan (1% in normal saline) was injected into the right hind paw of each rats. The paw volume was measured plethysmographically at 0, 0.5, 1, 2, 3, 4 and 5th hour after challenge. The percentage inhibition of edema was calculated for each group with respect to the vehicle received control group of animals. 
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 Percentage inhibition of inflammation in carrageenan  
               
               
                 induced paw oedema model. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Percentage  
               
               
                   
                   
                 inhibition (%) 
               
            
           
           
               
               
               
               
               
               
            
               
                 Groups 
                 Treatment 
                 0.5 hr 
                 1 hr 
                 2 hr 
                 3 hr 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Group I 
                 Control (1% Tween 80 v/v p.o.). 
                 0 
                 0 
                 0 
                 0 
               
               
                 Group II 
                 Ashwagandha root extract with 5% 
                 7 
                 12 
                 15 
                 18 
               
               
                   
                 withanolide glycosides (50 mg/kg 
                   
                   
                   
                   
               
               
                   
                 p.o.). 
                   
                   
                   
                   
               
               
                 Group III 
                 Ashwagandha root extract with 5% 
                 9 
                 16 
                 22 
                 26 
               
               
                   
                 withanolide glycosides coated with 
                   
                   
                   
                   
               
               
                   
                 Eudragit (50 mg/kg p.o.). 
                   
                   
                   
                   
               
               
                 Group IV 
                 Alkaloidal rich fraction of 
                 3 
                 8 
                 10 
                 13 
               
               
                   
                 Ashwagandha root extract  
                   
                   
                   
                   
               
               
                   
                 (50 mg/kg p.o.). 
                   
                   
                   
                   
               
               
                 Group V 
                 Purified Ashwagandha root extract 
                 20 
                 45 
                 57 
                 69 
               
               
                   
                 with 80% withanolide  
                   
                   
                   
                   
               
               
                   
                 glycoside ( 50 mg/kg p.o.). 
                   
                   
                   
                   
               
               
                 Group VI 
                 Purified Ashwagandha root extract 
                 24 
                 68 
                 75 
                 89 
               
               
                   
                 with 80% withanolide glycoside 
                   
                   
                   
                   
               
               
                   
                 coated with Eudragit  
                   
                   
                   
                   
               
               
                   
                 (50 mg/kg p.o.). 
                   
                   
                   
                   
               
               
                 Group VII 
                 Ashwagandha root extract with  
                 13 
                 19 
                 26 
                 33 
               
               
                   
                 3.5% withanolide glycosides  
                   
                   
                   
                   
               
               
                   
                 (50 mg/kg p.o.). 
                   
                   
                   
                   
               
               
                 Group VIII 
                 Ashwagandha root extract with  
                 20 
                 32 
                 40 
                 52 
               
               
                   
                 3.5% withanolide glycosides coated 
                   
                   
                   
                   
               
               
                   
                 with Eudragit (50 mg/kg p.o.). 
                   
                   
                   
                   
               
               
                 Group IX 
                 Indomethacin (10 mg/kg p.o.). 
                 28 
                 65 
                 80 
                 86 
               
               
                   
               
            
           
         
       
     
     The maximum percentage inhibition was shown at 3 hour after carrageenan injection. Maximum percentage inhibition of inflammation was observed in animals fed with indomethacin. Among the extracts treated groups, purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit was found to be most effective in reducing the inflammation in rat paw. 
     Ashwagandha root extract with 3.5% withanolide glycoside coated with Eudragit was also found to be effective in reducing the inflammation in rat paw. 
     Example 17 
     Toxicity Study in Rats 
     Twenty four albino rats (14 males and 14 females) weighing 200-250 g were used for this study. The rats were randomly divided in to six groups having two males and two females in each group. The animals were acclimatized for 7 days before the commencement of extract feeding. Following was the grouping of animals (Table 1): 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Grouping of animals in toxicity study. 
               
            
           
           
               
               
               
            
               
                   
                   
                 No. of 
               
               
                 S. 
                   
                 rats 
               
            
           
           
               
               
               
               
            
               
                 No. 
                 Group 
                 M 
                 F 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                 1. 
                 Ashwagandha root extract with 5% withanolide  
                 2 
                 2 
               
               
                   
                 glycosides (sample 2 prepared as per example 6) 
                   
                   
               
               
                 2. 
                 Ashwagandha root extract with 5% withanolide  
                 2 
                 2 
               
               
                   
                 glycosides coated with Eudragit (prepared as per example 8) 
                   
                   
               
               
                 3. 
                 Alkaloidal rich fraction of Ashwagandha root  
                 2 
                 2 
               
               
                   
                 extract (sample 2 prepared as per example 1) 
                   
                   
               
               
                 4. 
                 Purified Ashwagandha root extract with 80%  
                 2 
                 2 
               
               
                   
                 withanolide glycoside (sample 5 prepared as per example 5) 
                   
                   
               
               
                 5. 
                 Purified Ashwagandha root extract with 80%  
                 2 
                 2 
               
               
                   
                 withanolide glycoside (sample 5 prepared as per example 5)  
                   
                   
               
               
                   
                 coated with Eudragit (prepared as per example 8) 
                   
                   
               
               
                 6. 
                 Control (1% Tween 80) 
                 2 
                 2 
               
               
                   
               
            
           
         
       
     
     The test samples were administered orally at the dose level of 1000 mg/kg body weight daily with the help of a cannula attached with syringe. Similarly, a group of two male and two female rats were treated with vehicle i.e. 1% Tween 80, and were designated as the control group. The animals were observed for a total period of 14 days. Body weight of rats, food consumption, respiration, rectal temperature, general behavior and mortality/severe disability was recorded. 
     Results 
     Ashwagandha root extract with 5% withanolide glycosides, Ashwagandha root extract with 5% withanolide glycosides coated with Eudragit as well as alkaloidal rich fraction of Ashwagandha root extract were found to have toxic effects on rats. The animals were more docile and allowed free handling. Among all the groups, the animals fed with alkaloidal rich fraction had decrease in spontaneous movements, sluggish response to stimuli and diminished muscle tone. Power to maintain righting reflex was present. Whereas there were no toxic symptoms in rats fed with purified Ashwagandha root extract with 80% withanolide glycoside, purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit and control group. 
     Loss of appetite was also observed in the rats fed with Ashwagandha root extract with 5% withanolide glycosides or alkaloidal rich fraction whereas the animals fed with purified Ashwagandha root extract with 80% withanolide glycoside, purified Ashwagandha root extract with 80% withanolide glycoside after hydrolysis and purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit showed normal appetite which was comparable to control animals. Individual body weights were recorded on day 1, day 7 and day 14 of the study. The gain in body weights of Ashwagandha root extract with 5% withanolide glycosides and alkaloidal rich fraction treated animals was very less whereas the gain in body weight of purified Ashwagandha root extract with 80% withanolide glycoside and purified Ashwagandha root extract with 80% withanolide glycoside coated with Eudragit treated group was comparable to the weight gain of control animals. One rat (female) in Ashwagandha root extract group and three rats (two females and one male) in alkaloidal rich fraction group were died due to clonic convulsions and respiratory depression. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Feed consumption data 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 Average daily feed 
               
               
                   
                 S. 
                   
                 consumption (gm) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 No. 
                 Group 
                 Male 
                 Female 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 1. 
                 Ashwagandha root extract with  
                 25.75 
                 22.68 
               
               
                   
                   
                 5% withanolide glycosides 
                   
                   
               
               
                   
                 2. 
                 Ashwagandha root extract with  
                 26.22 
                 24.66 
               
               
                   
                   
                 5% withanolide glycosides 
                   
                   
               
               
                   
                   
                 coated with Eudragit 
                   
                   
               
               
                   
                 3. 
                 Alkaloidal rich fraction of  
                 16.24 
                 13.33 
               
               
                   
                   
                 Ashwagandha root extract 
                   
                   
               
               
                   
                 4. 
                 Purified Ashwagandha root extract  
                 38.15 
                 36.92 
               
               
                   
                   
                 with 80% withanolide glycoside 
                   
                   
               
               
                   
                 6. 
                 Purified Ashwagandha root extract  
                 37.92 
                 37.05 
               
               
                   
                   
                 with 80% withanolide 
                   
                   
               
               
                   
                   
                 glycoside coated with Eudragit 
                   
                   
               
               
                   
                 7. 
                 Control (1% Tween 80) 
                 38.55 
                 37.13 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Body weight (gm) of rats. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Average Body weight (gm) 
               
            
           
           
               
               
               
               
               
            
               
                 S. 
                   
                 Day 1 
                 Day 7 
                 Day 14 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 No. 
                 Group 
                 M 
                 F 
                 M 
                 F 
                 M 
                 F 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 1. 
                 Ashwagandha root extract with 5% 
                 214.5 
                 211.5 
                 210 
                 202 
                 198 
                 189 
               
               
                   
                 withanolide glycosides 
                   
                   
                   
                   
                   
                   
               
               
                 2. 
                 Ashwagandha root extract with 5% 
                 215.5 
                 212 
                 211 
                 210 
                 202.5 
                 195.5 
               
               
                   
                 withanolide glycosides coated with 
                   
                   
                   
                   
                   
                   
               
               
                   
                 Eudragit 
                   
                   
                   
                   
                   
                   
               
               
                 3. 
                 Alkaloidal rich fraction of Ashwagandha 
                 217 
                 210.5 
                 205.5 
                 201 
                 192 
                 187 
               
               
                   
                 root extract 
                   
                   
                   
                   
                   
                   
               
               
                 4. 
                 Purified Ashwagandha root extract with 
                 215.5 
                 207 
                 223 
                 213.5 
                 227 
                 219 
               
               
                   
                 80% withanolide glycoside 
                   
                   
                   
                   
                   
                   
               
               
                 6. 
                 Purified Ashwagandha root extract with 
                 218 
                 209 
                 224 
                 214.5 
                 229 
                 220 
               
               
                   
                 80% withanolide glycoside coated with 
                   
                   
                   
                   
                   
                   
               
               
                   
                 Eudragit 
                   
                   
                   
                   
                   
                   
               
               
                 7. 
                 Control (1% Tween 80) 
                 216 
                 210.5 
                 222.5 
                 216 
                 228 
                 221.5 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Behavioral and clinical parameters. 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 Rectal 
                   
                   
               
               
                 S. 
                 Group 
                 Respiration 
                 temperature 
                 General behavior 
                 Death 
               
               
                   
               
               
                 1. 
                 Ashwagandha  
                 Mild 
                 Decreased 
                 Mild depression  
                 1/4 
               
               
                   
                 root 
                 depression  
                 by 2° C. 
                 of general  
                   
               
               
                   
                 extract with 5% 
                   
                   
                 activity. Prefers 
                   
               
               
                   
                 withanolide  
                   
                   
                 to sit quietly. Very 
                   
               
               
                   
                 glycosides 
                   
                   
                 cooperative. 
                   
               
               
                 2. 
                 Ashwagandha  
                 Mild 
                 Decreased 
                 Sitting quietly. 
                 1/4 
               
               
                   
                 root 
                 depression 
                 by 2° C. 
                 Cooperative while 
                   
               
               
                   
                 extract with 5% 
                   
                   
                 handling 
                   
               
               
                   
                 withanolide 
                   
                   
                   
                   
               
               
                   
                 glycosides 
                   
                   
                   
                   
               
               
                   
                 coated with  
                   
                   
                   
                   
               
               
                   
                 Eudragit 
                   
                   
                   
                   
               
               
                 3. 
                 Alkaloidal  
                 Moderate 
                 Decreased 
                 General activity 
                 3/4 
               
               
                   
                 rich fraction of  
                 depression 
                 by 3° C. 
                 markedly depressed. 
                   
               
               
                   
                 Ashwagandha  
                   
                   
                 Prefers to lie in one 
                   
               
               
                   
                 root 
                   
                   
                 corner undisturbed. 
                   
               
               
                   
                 extract 
                   
                   
                 Sluggish response to 
                   
               
               
                   
                   
                   
                   
                 stimuli and  
                   
               
               
                   
                   
                   
                   
                 diminished 
                   
               
               
                   
                   
                   
                   
                 muscle tone. 
                   
               
               
                 4. 
                 Purified  
                 Normal 
                 No 
                 Normal behavior 
                 0/4 
               
               
                   
                 Ashwagandha 
                 respiration 
                 decrease 
                 comparable  
                   
               
               
                   
                 root extract  
                   
                   
                 to control 
                   
               
               
                   
                 with 80% 
                   
                   
                 rats. 
                   
               
               
                   
                 withanolide  
                   
                   
                   
                   
               
               
                   
                 glycoside 
                   
                   
                   
                   
               
               
                 6. 
                 Purified  
                 Normal 
                 No 
                 Normal behavior 
                 0/4 
               
               
                   
                 Ashwagandha 
                 respiration 
                 decrease 
                 comparable 
                   
               
               
                   
                 root extract  
                   
                   
                 to control 
                   
               
               
                   
                 with 80% 
                   
                   
                 rats. 
                   
               
               
                   
                 withanolide  
                   
                   
                   
                   
               
               
                   
                 glycoside 
                   
                   
                   
                   
               
               
                   
                 coated with  
                   
                   
                   
                   
               
               
                   
                 Eudragit 
                   
                   
                   
                   
               
               
                 7. 
                 Control (1%  
                 Normal 
                 No 
                 Normal  
                 0/4 
               
               
                   
                 Tween 80) 
                 respiration 
                 decrease 
                 behavior 
               
               
                   
               
            
           
         
       
     
     Example 18 
     The conversion of withanolide glycosides into aglycon moiety and sugar was studied in simulated gastric fluid (pH 1.2) by in vitro USP dissolution apparatus (LABINDIA DS 8000). The dissolution medium (simulated gastric fluid without enzyme, pH 1.2), free from dissolved air, was filled into the vessel of the dissolution apparatus. Apparatus was assembled and dissolution medium was heated to 36.5° to 37.5°. The enteric coated tablet (prepared as per example 9) containing alkaloid free purified Ashwagandha root extract (sample 5 prepared as per example 5) was sunk to the bottom of one vessel prior to the rotation of the paddle. In another vessel tablet without enteric coating containing purified Ashwagandha root extract (sample 5 prepared as per example 5) was sunk to the bottom of vessel prior to the rotation of the paddle. A suitable device such as a wire of glass helix was used to keep horizontal at the bottom of the vessel tablet that would otherwise float. 
     An aliquot of the liquid was collected from two vessels at 15, 30, 60, 90 and 120 minutes. The samples were transferred into a liquid-liquid extractor and extracted with chloroform-methanol mixture (80:20). Acidic and chloroform-methanol phases were separated. Chloroform-methanol phase was collected and acidic phase was again extracted with chloroform-methanol two more times. All the chloroform-methanol phases were pooled and extracted with water. Aqueous and chloroform-methanol phases were separated and chloroform-methanol phase was collected. Chloroform-methanol phase was concentrated and dried to form powder of chloroform-methanol extract. Chloroform-methanol extract is dissolved in methanol and analysed by HPLC. 
     
       
         
           
               
               
            
               
                   
               
               
                   
                 Percentage of aglycone withanolide 
               
            
           
           
               
               
               
            
               
                   
                 Enteric coated tablet of 
                 Tablet of purified  
               
               
                   
                 alkaloid free purified 
                 Ashwagandha 
               
               
                 Time 
                 Ashwagandha root 
                 root extract without  
               
               
                 (Min) 
                 extract 
                 enteric coating 
               
               
                   
               
            
           
           
               
               
               
            
               
                 0 
                 0 
                 0 
               
               
                 15 
                 1.2 
                 33.8 
               
               
                 30 
                 2.3 
                 46.7 
               
               
                 60 
                 3.6 
                 59.8 
               
               
                 90 
                 4.1 
                 65.3 
               
               
                 120 
                 4.8 
                 74.8 
               
               
                   
               
            
           
         
       
     
     It was found that at 15 minutes in acidic medium withanolide glycosides in tablet of purified Ashwagandha root extract without enteric coating was converted into withanolide aglycones (33.8%), but only 1.2 percent of withanolide aglycones were formed following the administration of enteric coated tablet of alkaloid free purified Ashwagandha root extract. At 2 hours 74.8 percent of withanolide aglycones were formed in the acidic environment following the administration of tablet of purified Ashwagandha root extract without enteric coating. After administration of enteric coated tablet of alkaloid free purified Ashwagandha root extract only 4.8 percent of withanolide aglycones were formed in the acidic condition. Withanolide glycosides undergo hydrolysis in acidic pH and get converted to withanolide aglycones which is toxic. The enteric coating prevented the hydrolysis of active compound like withanolide glycoside to aglycones in the Ashwagandha root extract in the acidic environment. 
     Example 19 
     Method of Making Ashwagandha Extract with 3.5% Withanolide Glycoside. 
     Roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were treated with 2% ammonia solution in a ratio of 1:2 solvent:roots of Ashwagandha for 4 hrs. Ammonia treated Ashwagandha roots were filled in the Soxhlet extractor and extracted with dichloromethane or methylene dichloride (MDC) (300 L). The extraction was carried out for 10 hrs at a temperature of about 70° C. After the completion of extraction, the supernatant and residue were separated by filtration. Residue (roots of Ashwagandha) after MDC extraction was washed with water till the pH become neutral. Then dried the root in a vacuum oven at 90-100° C. After drying the roots of Ashwagandha was powdered to form a powder of roots of Ashwagandha (sample 1) (88%). 
     88 Kg of powder of roots of Ashwagandha (sample 1) was extracted with 20% ethanol. Sample 1 was refluxed with 20% ethanol (300 L) at the boiling temperature (75-80° C.) of ethanol for one hour to obtain a second residue and second supernatant. The second residue was then further extracted two more times with three times the quantity of ethanol at each time. The residue and supernatants were separated. All the supernatants were pooled and concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated ethanol extract. The concentrated ethanol extract was filtered to get filtrate and third residue. 35% maltodextrin was added to the filtrate and spray dried to get powder of 20% ethanol extract of Ashwagandha. The yield was about 18% (Sample 2). 
     See also extract preparation in  FIG.  7   . 
     The withanolide glycoside content in powder of ammonia treated 20% ethanol extract of Ashwagandha was about 3.5% by HPLC. Saponin content in powder of ammonia treated 20% ethanol extract of Ashwagandha was about 2.5% by UV method. Alkaloid content in powder of ammonia treated 20% methanol extract of Ashwagandha was about 0.06% by gravimetry method. Withanolide aglycones content in powder of ammonia treated 20% ethanol extract of Ashwagandha was about 0.15% by HPLC. Oligosaccharide content in powder of ammonia treated 20% ethanol extract of Ashwagandha was about 3% by HPLC. 
     Example 20 
     Method of Making Enteric Coated Ashwagandha Root Extract Granules with 3.5% Withanolide Glycoside. 
     1 Kg of Ashwagandha root extract powder with 3.5% withanolide glycoside was passed through the Roll Compactor machine. The flakes obtained from the roll compactor were passed through an Oscillating Granulator machine fitted with 16 mesh screen to obtain granules of Ashwagandha root extract. 
     Granules of Ashwagandha root extract was loaded into the bowl of the fluid bed extractor (pam glatt pharma technologies). The bowl has a fine Stainless steel mesh at the bottom. The air used for drying/fluidizing was successively filtered through HEPA (High-efficiency particulate arrestance) filters (EU 13 grade, 0.3 micron rating, 99.997% efficiency). 
     Hot, filtered air up to 90° C. was passed at high velocity from the bottom of the FBE bowl through the feed material (Ashwagandha root extract with 3.5% Withanolide glycoside) and feed material was fluidised. 
     Meantime, 100 g coating material (Poly-methacrylic acid-co-methyl methacrylate (Eudragit) was dissolved in 900 ml water. Coating solution was sprayed into fluidised material by using a spraying devise attached to the FBE (spray speed 0.5 L in 1 Hr, pump rpm range 10-12). Through the process of fluid bed coating, fluidized particles are continuously sprayed with coating solution, depositing layers (films) of material to the surface of the particles, and yielding an even layer, 4% weight gain with 6 mg/cm 2  thickness. 
     Example 21 
     Method of Making Minitablet of Ashwagandha Root Extract with 3.5% Withanolide Glycoside. 
     5 Kg powder of 20% ethanol extract of Ashwagandha was mixed with 19% Micro Crystalline Cellulose (MCC) and 1% Magnesium stearate. So the loading of the original extract was 80%. This mixture was fed in tableting machine having 3 mm dies and punches to get the 3 mm diameter mini tablets with 9 Kg/cm 2  hardness. 
     Example 22 
     Method of Making Enteric Coated Mini Tablet of Ashwagandha Root Extract with 3.5% Withanolide Glycoside. 
     The mini tablets prepared as per example 21 were coated by Pan coating method. In brief, 4 Kg mini tablets with 3 mm diameter were introduced into the Pan and rotated at 20 rpm. Coating material (EUDRAGUARD brand enteric coating polymer from Evonik) was sprayed on to the tablets via a spray gun as 30% aqueous dispersion. Simultaneously hot blower (110 degree) was started for fast drying of the tablets. Tablets were coated till a weight gain of 12%. After coating, tablets were further kept in hot air stream for 15 minutes to ensure complete drying. 
     Example 23 
     Roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were extracted with 100% methanol. Roots were refluxed with 100% methanol at the boiling temperature (60-70° C.) of methanol for one hour to obtain a first residue and first supernatant. The first residue was then further extracted two more times with four times the quantity of methanol at each time. The residue and supernatants were separated. All the supernatants were pooled and concentrated in an Agitated thin film evaporator (ATFE) to form a concentrated methanol extract. The concentrated methanol extract was dried under vacuum at above 500 mm of mercury to get powder of 100% methanol extract of Ashwagandha (yield 6%). 
     Withanolide glycoside content in powder of 100% methanol extract of Ashwagandha was 1% by HPLC 
     Example 24 
     Method of Preparation of Ashwagandha Root Powder. 
     Fresh roots of Ashwagandha were collected (100 Kg). Roots of Ashwagandha were cleaned. Cleaned roots were dried. Dried roots were pulverized to get powder of root Ashwagandha (Yield 70%). 
     Withanolide glycoside content in Ashwagandha powder was 0.6% by HPLC. 
     Example 25 
     Method of Analysis of Saponins by Spectrophotometric Method. 
     50 mg Ashwagandha extract was weighed into a 50 ml standard flask and make up to the volume by adding water. Standard was prepared by weighing accurately 5 mg standard [standard Protodioscin 97.2% purity from Chromadex] and transferred into a 5 ml standard flask and made up to a 5 ml solution with water. 1 ml of sample and 1 ml standard were pipette out into two separate test tubes. 1 ml of anisaldehyde reagent was added to each tube and mixed well. Mixture was kept for 10 minutes. 4 ml of 70% sulphuric acid reagent was added to each tube and mixed. Tubes were kept in water bath with constant temperature of 60° c. After 10 minutes, tubes were cooled and absorbance was taken at 435 nm. Saponins were determined by the formula 
     
       
         
           
             
               Total 
               ⁢ 
               
                 
                     
                 
                 ⁢ 
                 
                     
                 
               
               ⁢ 
               saponin 
             
             = 
             
               
                 
                   
                     
                       Absorbance 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       of 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       sample 
                       × 
                     
                   
                 
                 
                   
                     
                       Standard 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       concentration 
                       × 
                     
                   
                 
                 
                   
                     
                       purity 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       of 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       Standard 
                     
                   
                 
               
               
                 
                   
                     
                       Absorbance 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       of 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       Standard 
                       × 
                     
                   
                 
                 
                   
                     
                       sample 
                       ⁢ 
                       
                           
                       
                       ⁢ 
                       concentration 
                     
                   
                 
               
             
           
         
       
     
     Example 26 
     Method of Preparation of Different Ashwagandha Powder/Extract in Different pH. 
     Ashwagandha powder/extract was added to different buffer (pH 1, 2, 3, 5, 7 and 7.4) in 1:20 ratio of Ashwagandha powder/extract:buffer. The extract-buffer solution was kept in a waterbath at 37° C. for 2 hrs. The solution was neutralised by adding base or acid solution. The neutralised solution was concentrated and dried. 
     Example 27 
     Method of Making Amaranth Extract with 9% Nitrate. 
     Fresh Amaranth were collected (100 Kg). Leaves and stem of fresh Amaranth were cleaned and crushed. Water in an amount ten times the quantity of crushed material of Amaranth was added to form a mixture. The extraction was performed using an extractor with a reflux condenser. The bottom of the extractor was fitted with a polypropylene (100 microns) filter cloth. The mixture was refluxed for one hour to obtain a first residue and supernatant. The residue and supernatants were separated by draining out the supernatant from the extractor bottom through the polypropylene filter cloth using a centrifugal pump. After the first extraction, the first residue was further extracted with ten times the quantity of water to get second residue and supernatant. The second residue was further extracted with ten times the quantity of water to get third residue and supernatant. All the supernatants were pooled and concentrated in an Agitated thin film evaporator (ATFE) at a temperature of 85° C. to form concentrated water extract (yield 3%). 
     Concentrated water extract was passed through a carbon column to obtain a filtrate. Filtrate was concentrated and clarified to form a supernatant and residue. Supernatant was dried under vacuum at above 500 mm of mercury to get powder of purified water extract of fresh Amaranth (Yield 2.5 Kg). 
     The nitrate content in water extract of fresh Amaranth by ion chromatography was found to be 9%. 
     Example 28 
     Method of Making Combination of Enteric Coated Ashwagandha Root Extract with 3.5% Withanolide Glycoside and Amaranth Extract with 9% Nitrate. 
     2.5 Kg of Enteric coated Ashwagandha extract prepared as per example 20 and 2.5 Kg of Amaranth extract prepared as per Example 25 was blended in 1:1 ratio by using Double Cone Blender (stainless steel SS-316, capacity 2000 litre, manufacturer: Zebra Pharma, Mumbai). 
     Example 29 
     Efficacy Study of Different Ashwagandha Extracts Granules and Powder Treated at Different pH. 
     108 rats were divided into 27 groups comprising of 4 rats in each group. The animals were trained for swimming test (15 minutes) as pre-test session and then fasted overnight. To the fasted animals, reserpine (6 mg/kg) was injected i.p. to depress the animals. After 1 hour of reserpine injection, the test samples treated at different pH/standard were fed orally as designated dose. After 1 hour of test sample/standard, the rats were tested using forced swim test and duration of immobility in the 5 minutes test session was recorded. In this test more immobility time indicate that animal is more depressed or stressed. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Effect of different Ashwagandha powder/extracts granules in  
               
               
                 different pH on Immobility time in rats. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 Immobility 
               
               
                   
                   
                   
                 time in 
               
               
                   
                 Groups 
                 Treatment 
                 seconds 
               
               
                   
                   
               
            
           
           
               
               
               
               
            
               
                   
                 Group I 
                 Normal control (Received only vehicle;  
                 120 
               
               
                   
                   
                 1% Tween 80 v/v p.o.). 
                   
               
               
                   
                 Group II 
                 Reserpine (6 mg/kg i.p) + Vehicle  
                 250 
               
               
                   
                   
                 (1% Tween 80 v/v p.o.) 
                   
               
               
                   
                 Group III 
                 Reserpine (6 mg/kg i.p) +  
                 245 
               
               
                   
                   
                 Ashwagandha root powder (Prepared 
                   
               
               
                   
                   
                 as per example 24) at pH1(Prepared  
                   
               
               
                   
                   
                 as per example 26) 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group IV 
                 Reserpine (6 mg/kg i.p) +  
                 245 
               
               
                   
                   
                 Ashwagandha root powder at pH2 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group V 
                 Reserpine (6 mg/kg i.p) +  
                 230 
               
               
                   
                   
                 Ashwagandha root powder at pH3 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group VI 
                 Reserpine (6 mg/kg i.p) +  
                 220 
               
               
                   
                   
                 Ashwagandha root powder at pH5 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group VII 
                 Reserpine (6 mg/kg i.p) +  
                 205 
               
               
                   
                   
                 Ashwagandha root powder at pH7 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group VIII 
                 Reserpine (6 mg/kg i.p) +  
                 205 
               
               
                   
                   
                 Ashwagandha root powder at pH7.4 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group IX 
                 Reserpine (6 mg/kg i.p) +  
                 235 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides(prepared  
                   
               
               
                   
                   
                 as per example 19) at 
                   
               
               
                   
                   
                 pH1(prepared as per example 26)  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group X 
                 Reserpine (6 mg/kg i.p) +  
                 230 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides at pH2  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group XI 
                 Reserpine (6 mg/kg i.p) +  
                 210 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides at pH 3  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group XII 
                 Reserpine (6 mg/kg i.p) +  
                 189 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides at pH 5  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group XIII 
                 Reserpine (6 mg/kg i.p) +  
                 152 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides at pH 7  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group XIV 
                 Reserpine (6 mg/kg i.p) +  
                 150 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides at pH 7.4  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group XV 
                 Reserpine (6 mg/kg i.p) +  
                 220 
               
               
                   
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                   
                 withanolide glycosides(sample 3  
                   
               
               
                   
                   
                 prepared as per example 4) at 
                   
               
               
                   
                   
                 pH 1(Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group XVI 
                 Reserpine (6 mg/kg i.p) +  
                 220 
               
               
                   
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                   
                 prepared as per example 4)at 
                   
               
               
                   
                   
                 pH 2(prepared as per example  
                   
               
               
                   
                   
                 26(Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group 
                 Reserpine (6 mg/kg i.p) +  
                 202 
               
               
                   
                 XVII 
                 Ashwagandha extract with 35% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 3(Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group 
                 Reserpine 6 mg/kg i.p) +  
                 180 
               
               
                   
                 XVIII 
                 Ashwagandha extract with 35% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 5(Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group XIX 
                 Reserpine 6 mg/kg i.p) +  
                 145 
               
               
                   
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 7 (Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group XX 
                 Reserpine 6 mg/kg i.p) +  
                 139 
               
               
                   
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 7.4 (Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 GroupXI 
                 Reserpine 6 mg/kg i.p) +  
                 212 
               
               
                   
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                   
                 withanolide glycosides(sample 5  
                   
               
               
                   
                   
                 prepared as per example 5) at 
                   
               
               
                   
                   
                 pH 1(prepared as per example  
                   
               
               
                   
                   
                 26)(Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 GroupXII 
                 Reserpine (6 mg/kg i.p) +  
                 211 
               
               
                   
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 2(Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 GroupXIII 
                 Reserpine 6 mg/kg i.p) +  
                 194 
               
               
                   
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 3(Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group XIV 
                 Reserpine 6 mg/kg i.p) +  
                 165 
               
               
                   
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 5(Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group XV 
                 Reserpine 6 mg/kg i.p) +  
                 110 
               
               
                   
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 7 (Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group XVI 
                 Reserpine 6 mg/kg i.p) +  
                 105 
               
               
                   
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                   
                 withanolide glycosides at  
                   
               
               
                   
                   
                 pH 7.4 (Dosage 20 mg/kg p.o). 
                   
               
               
                   
                 Group 
                 Reserpine(6 mg/kg i.p) +  
                 120 
               
               
                   
                 XVII 
                 Fluoxetine (10 mg/kg p.o.). 
               
               
                   
                   
               
            
           
         
       
     
     As shown in the Table 1, immobility time for normal control animals was 120 seconds. When reserpine was injected i.p., the animals got depressed and immobility time increased to 250 seconds (Group II). Oral administration of Ashwagandha root powders (200 mg/kg) at pH 1, 2, 3 and 5 after reserpine injection was not much effective and immobility time was recorded as 245, 245, 230 and 220 seconds respectively (Group III to VI). Administration of Ashwagandha root powders (200 mg/kg) at pH 7 and 7.4 after reserpine injection was slightly effective and immobility time was recorded as 205 seconds (Group VII to VIII). Similarly, administration of Ashwagandha extract with 3.5% withanolide glycosides (60 mg/kg) at pH 1, 2, 3 and 5 after reserpine injection was slightly effective (better than ashwagandha root powder) and immobility time was recorded as 235, 230, 210 and 189 seconds respectively (Group IX to XII). Whereas, administration of Ashwagandha extract with 3.5% withanolide glycosides (60 mg/kg) at pH 7 and 7.4 after reserpine injection was more effective and immobility time was recorded as 152 and 150 seconds respectively (Group XIII to XIV). 
     Administration of Ashwagandha extract with 35% withanolide glycosides (20 mg/kg) at pH 1, 2, 3 and 5 after reserpine injection was effective and immobility time was recorded as 220, 220, 202 and 180 seconds respectively (Group XV to XVIII). Whereas, administration of Ashwagandha extract with 35% withanolide glycosides (20 mg/kg) at pH 7 and 7.4 after reserpine injection was more effective and immobility time was recorded as 145 and 139 seconds respectively (Group XIX to XX). 
     In case of administration of extracts containing very high (80%) withanolide glycosides (20 mg/kg) at pH 1, 2, 3 and 5 after reserpine injection was effective and immobility time was recorded as 212, 211, 194 and 165 seconds respectively (Group XXI to XIV). Whereas, administration of Ashwagandha extract with 80% withanolide glycosides (20 mg/kg) at pH 7 and 7.4 after reserpine injection was most effective and immobility time was recorded as 110 and 105 seconds respectively (Group XV to XVI). In case of fluoxetine standard at 10 mg/kg (Group XVII), the immobility time was only 122 seconds which is almost similar to normal control animals. 
     Example 30 
     Efficacy Study of Different Ashwagandha Extracts Granules with Normal (Film), Enteric and Delayed Release Coating. 
     60 rats were divided into 15 groups comprising of 4 rats in each group. The animals were trained for swimming test (15 minutes) as pre-test session and then fasted overnight. To the fasted animals, reserpine (6 mg/kg) was injected i.p. to depress the animals. Immediately after reserpine injection, the test samples with normal (Hydroxy Propyl Cellulose; HPC), enteric (Eudragit) and delayed release (High percentage of Eudragit) coating/standard were fed orally as designated doses. After 2 hour of test sample/standard, the rats were tested using forced swim test and duration of immobility in the 5 minutes test session was recorded. In this test more immobility time indicate that animal is more depressed or stressed. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Effect of different Ashwagandha powder/granules with normal/ 
               
               
                 enteric/delayed release coating on Immobility time in rats. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Immobility 
               
               
                   
                   
                 time in 
               
               
                 Groups 
                 Treatment 
                 seconds 
               
               
                   
               
               
                 Group I 
                 Normal control (Received only vehicle;  
                 120 
               
               
                   
                 1% Tween 80 v/v p.o.). 
                   
               
               
                 Group II 
                 Reserpine (6 mg/kg i.p) + Vehicle  
                 250 
               
               
                   
                 (1% Tween 80 v/v p.o.). 
                   
               
               
                 Group III 
                 Reserpine (6 mg/kg i.p) +  
                 245 
               
               
                   
                 Ashwagandha root powder 
                   
               
               
                   
                 (Prepared as per example 24)with  
                   
               
               
                   
                 normal HPC coating 
                   
               
               
                   
                 (Dosage 200 mg/kg p.o).. 
                   
               
               
                 Group IV 
                 Reserpine (6 mg/kg i.p) +  
                 205 
               
               
                   
                 Ashwagandha root powder 
                   
               
               
                   
                 (Prepared as per example 24)with  
                   
               
               
                   
                 enteric coating (Dosage 
                   
               
               
                   
                 200 mg/kg p.o). 
                   
               
               
                 Group V 
                 Reserpine (6 mg/kg i.p) +  
                 220 
               
               
                   
                 Ashwagandha root powder 
                   
               
               
                   
                 (Prepared as per example 24)with  
                   
               
               
                   
                 delayed release coating 
                   
               
               
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                 Group VI 
                 Reserpine (6 mg/kg i.p) + Ashwagandha  
                 215 
               
               
                   
                 granules with 3.5% 
                   
               
               
                   
                 withanolide glycosides (sample 2  
                   
               
               
                   
                 prepared as per example 
                   
               
               
                   
                 19) with normal HPC coating  
                   
               
               
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                 Group VII 
                 Reserpine (6 mg/kg i.p) +  
                 141 
               
               
                   
                 Ashwagandha granules with 3.5% 
                   
               
               
                   
                 withanolide glycosides (sample 2  
                   
               
               
                   
                 prepared as per example 
                   
               
               
                   
                 19)with enteric coating(Dosage  
                   
               
               
                   
                 60 mg/kg p.o). 
                   
               
               
                 Group VIII 
                 Reserpine (6 mg/kg i.p) +  
                 186 
               
               
                   
                 Ashwagandha granules with 3.5% 
                   
               
               
                   
                 withanolide glycosides (sample  
                   
               
               
                   
                 2 prepared as per example 
                   
               
               
                   
                 19)with delayed release  
                   
               
               
                   
                 coating(Dosage 60 mg/kg p.o). 
                   
               
               
                 Group IX 
                 Reserpine (6 mg/kg i.p) +  
                 192 
               
               
                   
                 Ashwagandha granules with 35% 
                   
               
               
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                 prepared as per example 
                   
               
               
                   
                 4)with normal HPC coating  
                   
               
               
                   
                 (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group X 
                 Reserpine (6 mg/kg i.p) +  
                 138 
               
               
                   
                 Ashwagandha granules with 35% 
                   
               
               
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                 prepared as per example 
                   
               
               
                   
                 4) with enteric coating  
                   
               
               
                   
                 (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group XI 
                 Reserpine (6 mg/kg i.p) +  
                 162 
               
               
                   
                 Ashwagandha granules with 35% 
                   
               
               
                   
                 withanolide glycosides (sample  
                   
               
               
                   
                 3 prepared as per example 
                   
               
               
                   
                 4) with delayed release coating  
                   
               
               
                   
                 (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group XII 
                 Reserpine (6 mg/kg i.p) +  
                 173 
               
               
                   
                 Ashwagandha granules with 80% 
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 
                   
               
               
                   
                 5) with normal HPC coating  
                   
               
               
                   
                 (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group XIII 
                 Reserpine (6 mg/kg i.p) +  
                 109 
               
               
                   
                 Ashwagandha granules with 80% 
                   
               
               
                   
                 withanolide glycosides(sample 5  
                   
               
               
                   
                 prepared as per example 5) 
                   
               
               
                   
                 with enteric coating (Dosage 20  
                   
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group XIV 
                 Reserpine (6 mg/kg i.p) +  
                 132 
               
               
                   
                 Ashwagandha granules with 80% 
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 
                   
               
               
                   
                 5)with delayed release coating  
                   
               
               
                   
                 (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group XV 
                 Reserpine(6 mg/kg i.p) + Fluoxetine  
                 120 
               
               
                   
                 (10 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     As shown in the Table 1, immobility time for normal control animals was 120 seconds. When reserpine was injected i.p., the animals got depressed and immobility time was increased to 250 seconds (Group II). Oral administration of Ashwagandha root powder with normal HPC coating (200 mg/kg) after reserpine injection was not much effective and immobility time was recorded as 245 seconds (Group III). Administration of Ashwagandha root powder with enteric coating (200 mg/kg) after reserpine injection was more effective than HPC coating and immobility time was recorded as 205 seconds (Group IV). Administration of Ashwagandha root powder with delayed release coating (200 mg/kg) after reserpine injection was less effective than enteric coating and immobility time was recorded as 220 seconds (Group V). 
     Similarly, oral administration of Ashwagandha granules with 3.5% withanolide glycosides with normal HPC coating (60 mg/kg) after reserpine injection was not much effective and immobility time was recorded as 215 seconds (Group VI). Administration of Ashwagandha granules with 3.5% withanolide glycosides with enteric coating (60 mg/kg) after reserpine injection was more effective than HPC coating and immobility time was recorded as 141 seconds (Group VII). Administration of Ashwagandha granules with 3.5% withanolide glycosides with delayed release coating (60 mg/kg) after reserpine injection was less effective than enteric coating and immobility time was recorded as 186 seconds (Group VIII). 
     Oral administration of Ashwagandha granules with 35% withanolide glycosides with normal HPC coating (20 mg/kg) after reserpine injection was slightly effective and immobility time was recorded as 192 seconds (Group IX). Administration of Ashwagandha granules with 35% withanolide glycosides with enteric coating (20 mg/kg) after reserpine injection was more effective than HPC coating and immobility time was recorded as 138 seconds (Group X). Administration of Ashwagandha granules with 35% withanolide glycosides with delayed release coating (20 mg/kg) after reserpine injection was less effective than enteric coating and immobility time was recorded as 162 seconds (Group XI). 
     Oral administration of Ashwagandha granules with 80% withanolide glycosides with normal HPC coating (20 mg/kg) after reserpine injection was effective and immobility time was recorded as 173 seconds (Group XII). Administration of Ashwagandha granules with 80% withanolide glycosides with enteric coating (20 mg/kg) after reserpine injection was most effective than HPC coating and immobility time was recorded as 109 seconds (Group XIII). Administration of Ashwagandha granules with 80% withanolide glycosides with delayed release coating (20 mg/kg) after reserpine injection was less effective than enteric coating and immobility time was recorded as 132 seconds (Group XIV). In case of fluoxetine standard at 10 mg/kg (Group XVII), the immobility time was 120 seconds which is similar to normal control animals. 
     Example 31 
     Efficacy Study of Different Ashwagandha Extracts Granules with Different Percentage of Enteric Coating. 
     44 rats were divided into 11 groups comprising of 4 rats in each group. The animals were trained for swimming test (15 minutes) as pre-test session and then fasted overnight. To the fasted animals, reserpine (6 mg/kg) was injected i.p. to depress the animals. Immediately after the reserpine injection, the test samples (ashwagandha root powder) with different percentage of enteric coating/standard were fed orally as designated dose. After 2 hour of test sample/standard, the rats were tested using forced swim test and duration of immobility in the 5 minutes test session was recorded. In this test more immobility time indicate that animal is more depressed or stressed. 
     The test was repeated with different percentage of coated ashwagandha extract containing 3.5% withanolide glycosides and different percentage of coated ashwagandha extract containing 35% and 80% withanolide glycosides. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha powder with different percentage of  
               
               
                 enteric coating on Immobility time in rats. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 Immobility 
               
               
                   
                   
                   
                 time in 
               
               
                   
                 Groups 
                 Treatment 
                 seconds 
               
               
                   
                   
               
               
                   
                 Group I 
                 Normal control (Received only vehicle;  
                 120 
               
               
                   
                   
                 1% Tween 80 v/v p.o.). 
                   
               
               
                   
                 Group II 
                 Reserpine (6 mg/kg i.p) + Vehicle  
                 250 
               
               
                   
                   
                 (1% Tween 80 v/v p.o.). 
                   
               
               
                   
                 Group III 
                 Reserpine (6 mg/kg i.p) +  
                 245 
               
               
                   
                   
                 Ashwagandha root powder 
                   
               
               
                   
                   
                 (Prepared as per example 24)with  
                   
               
               
                   
                   
                 0.5% enteric coating 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group IV 
                 Reserpine (6 mg/kg i.p) +  
                 235 
               
               
                   
                   
                 Ashwagandha root powder 
                   
               
               
                   
                   
                 (Prepared as per example 24)with  
                   
               
               
                   
                   
                 1% enteric coating 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group V 
                 Reserpine (6 mg/kg i.p) +  
                 223 
               
               
                   
                   
                 Ashwagandha root powder 
                   
               
               
                   
                   
                 (Prepared as per example 24)  
                   
               
               
                   
                   
                 with 3% enteric coating 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group VI 
                 Reserpine (6 mg/kg i.p) +  
                 216 
               
               
                   
                   
                 Ashwagandha root powder 
                   
               
               
                   
                   
                 (Prepared as per example 24)with  
                   
               
               
                   
                   
                 5% enteric coating 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group VII 
                 Reserpine (6 mg/kg i.p) +  
                 207 
               
               
                   
                   
                 Ashwagandha root powder with 
                   
               
               
                   
                   
                 7% enteric coating  
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group VIII 
                 Reserpine (6 mg/kg i.p) +  
                 201 
               
               
                   
                   
                 Ashwagandha root powder 
                   
               
               
                   
                   
                 (Prepared as per example 24)with  
                   
               
               
                   
                   
                 10% enteric coating 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group IX 
                 Reserpine (6 mg/kg i.p) +  
                 200 
               
               
                   
                   
                 Ashwagandha root powder with 
                   
               
               
                   
                   
                 12% enteric coating  
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group X 
                 Reserpine (6 mg/kg i.p) +  
                 200 
               
               
                   
                   
                 Ashwagandha root powder 
                   
               
               
                   
                   
                 (Prepared as per example 24)  
                   
               
               
                   
                   
                 with 15% enteric coating 
                   
               
               
                   
                   
                 (Dosage 200 mg/kg p.o). 
                   
               
               
                   
                 Group XI 
                 Reserpine(6 mg/kg i.p) +  
                 120 
               
               
                   
                   
                 Fluoxetine (10 mg/kg p.o.). 
               
               
                   
                   
               
            
           
         
       
     
     As shown in the Table 1, immobility time for normal control animals was 120 seconds. When reserpine was injected i.p., the animals got depressed and immobility time was increased to 250 seconds (Group II). Oral administration of Ashwagandha root powder with 0.5%, 1% and 3% enteric coating (200 mg/kg) after reserpine injection was not much effective and immobility time was recorded as 245, 235 and 223 seconds respectively (Group III to V). Administration of Ashwagandha root powder with 5 and 7% enteric coating (200 mg/kg) after reserpine injection was more effective than lower percentage of coatings and immobility time was recorded as 216 and 207 seconds respectively (Group VI to VII). Administration of Ashwagandha root powder with 10, 12 and 15% enteric coating (200 mg/kg) after reserpine injection was most effective and immobility time was recorded as 201, 200 and 200 seconds respectively (Group VIII to X). In case of fluoxetine standard at 10 mg/kg (Group XI), the immobility time was 120 seconds which is similar to normal control animals. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha extract with 3.5%  
               
               
                 withanolide glycoside with different 
               
               
                 percentage of enteric coating on Immobility time in rats. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 Immobility 
               
               
                   
                   
                   
                 time in 
               
               
                   
                 Groups 
                 Treatment 
                 seconds 
               
               
                   
                   
               
               
                   
                 Group I 
                 Normal control (Received only vehicle;  
                 120 
               
               
                   
                   
                 1% Tween 80 v/v p.o.). 
                   
               
               
                   
                 Group II 
                 Reserpine (6 mg/kg i.p) +  
                 250 
               
               
                   
                   
                 Vehicle (1% Tween 80 v/v p.o.). 
                   
               
               
                   
                 Group III 
                 Reserpine (6 mg/kg i.p) +  
                 225 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides (sample 2  
                   
               
               
                   
                   
                 prepared as per example 19) 
                   
               
               
                   
                   
                 with 0.5% enteric coating  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group IV 
                 Reserpine (6 mg/kg i.p) +  
                 220 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides (sample  
                   
               
               
                   
                   
                 2 prepared as per example 
                   
               
               
                   
                   
                 19)with 1% enteric coating  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group V 
                 Reserpine (6 mg/kg i.p) +  
                 206 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides (sample 2  
                   
               
               
                   
                   
                 prepared as per example 19) 
                   
               
               
                   
                   
                 with 3% enteric coating  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group VI 
                 Reserpine (6 mg/kg i.p) +  
                 195 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides (sample  
                   
               
               
                   
                   
                 2 prepared as per example 
                   
               
               
                   
                   
                 19)with 5% enteric coating  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group VII 
                 Reserpine (6 mg/kg i.p) +  
                 180 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides (sample  
                   
               
               
                   
                   
                 2 prepared as per example 19) 
                   
               
               
                   
                   
                 with 7% enteric coating  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group VIII 
                 Reserpine (6 mg/kg i.p) +  
                 160 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides with  
                   
               
               
                   
                   
                 10% enteric coating (Dosage 60 
                   
               
               
                   
                   
                 mg/kg p.o). 
                   
               
               
                   
                 Group IX 
                 Reserpine (6 mg/kg i.p) +  
                 142 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides (sample 2  
                   
               
               
                   
                   
                 prepared as per example 19) 
                   
               
               
                   
                   
                 with 12% enteric coating  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 Group X 
                 Reserpine (6 mg/kg i.p) +  
                 140 
               
               
                   
                   
                 Ashwagandha extract with 3.5% 
                   
               
               
                   
                   
                 withanolide glycosides (sample  
                   
               
               
                   
                   
                 2 prepared as per example 
                   
               
               
                   
                   
                 19)with 15% enteric coating  
                   
               
               
                   
                   
                 (Dosage 60 mg/kg p.o). 
                   
               
               
                   
                 GroupXI 
                 Reserpine(6 mg/kg i.p) +  
                 120 
               
               
                   
                   
                 Fluoxetine (10 mg/kg p.o.). 
               
               
                   
                   
               
            
           
         
       
     
     As shown in the Table 2, immobility time for normal control animals was 120 seconds. When reserpine was injected i.p., the animals got depressed and immobility time was increased to 250 seconds (Group II). Oral administration of Ashwagandha extract with 3.5% withanolide glycosides with 0.5%, 1% and 3% enteric coating (60 mg/kg) after reserpine injection was not much effective and immobility time was recorded as 225, 220 and 206 seconds respectively (Group III to V). Administration of Ashwagandha extract with 3.5% withanolide glycosides with 5 and 7% enteric coating (60 mg/kg) after reserpine injection was more effective than lower percentage of coatings and immobility time was recorded as 195 and 180 seconds respectively (Group VI to VII). Administration of Ashwagandha extract with 3.5% withanolide glycosides with 10, 12 and 15% enteric coating (60 mg/kg) after reserpine injection was most effective and immobility time was recorded as 160, 142 and 140 seconds respectively (Group VIII to X). In case of fluoxetine standard at 10 mg/kg (Group XI), the immobility time was 120 seconds which is similar to normal control animals. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha extract with 35%  
               
               
                 withanolide glycoside with different 
               
               
                 percentage of enteric coating on Immobility time in rats. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Immobility 
               
               
                   
                   
                 time in 
               
               
                 Groups 
                 Treatment 
                 seconds 
               
               
                   
               
               
                 Group I 
                 Normal control (Received only vehicle;  
                 120 
               
               
                   
                 1% Tween 80 v/v p.o.). 
                   
               
               
                 Group II 
                 Reserpine (6 mg/kg i.p) +  
                 250 
               
               
                   
                 Vehicle (1% Tween 80 v/v p.o.). 
                   
               
               
                 Group III 
                 Reserpine (6 mg/kg i.p) +  
                 200 
               
               
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                 prepared as per example 4)with 
                   
               
               
                   
                 0.5% enteric coating  
                   
               
               
                   
                 (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group IV 
                 Reserpine (6 mg/kg i.p) +  
                 188 
               
               
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                 prepared as per example 4) with 
                   
               
               
                   
                 1% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group V 
                 Reserpine (6 mg/kg i.p) +  
                 180 
               
               
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                 prepared as per example 4) with 
                   
               
               
                   
                 3% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group VI 
                 Reserpine (6 mg/kg i.p) +  
                 171 
               
               
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                 prepared as per example 4) with 
                   
               
               
                   
                 5% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group VII 
                 Reserpine (6 mg/kg i.p) +  
                 160 
               
               
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides(sample 3  
                   
               
               
                   
                 prepared as per example 4) with 
                   
               
               
                   
                 7% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group 
                 Reserpine (6 mg/kg i.p) +  
                 153 
               
               
                 VIII 
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides with 10%  
                   
               
               
                   
                 enteric coating (Dosage 20 
                   
               
               
                   
                 mg/kg p.o). 
                   
               
               
                 Group IX 
                 Reserpine (6 mg/kg i.p) +  
                 139 
               
               
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                 prepared as per example 4)with 
                   
               
               
                   
                 12% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group X 
                 Reserpine (6 mg/kg i.p) +  
                 136 
               
               
                   
                 Ashwagandha extract with 35% 
                   
               
               
                   
                 withanolide glycosides (sample 3  
                   
               
               
                   
                 prepared as per example 4)with 
                   
               
               
                   
                 15% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 GroupXI 
                 Reserpine(6 mg/kg i.p) + Fluoxetine  
                 120 
               
               
                   
                 (10 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     As shown in the Table 3, immobility time for normal control animals was 120 seconds. When reserpine was injected i.p., the animals got depressed and immobility time was increased to 250 seconds (Group II). Oral administration of Ashwagandha extract with 35% withanolide glycosides with 0.5%, 1% and 3% enteric coating (20 mg/kg) after reserpine injection was not much effective and immobility time was recorded as 200, 188 and 180 seconds respectively (Group III to V). Administration of Ashwagandha extract with 35% withanolide glycosides with 5 and 7% enteric coating (20 mg/kg) after reserpine injection was more effective than lower percentage of coatings and immobility time was recorded as 171 and 160 seconds respectively (Group VI to VII). Administration of Ashwagandha extract with 35% withanolide glycosides with 10, 12 and 15% enteric coating (20 mg/kg) after reserpine injection was most effective and immobility time was recorded as 153, 139 and 136 seconds respectively (Group VIII to X). In case of fluoxetine standard at 10 mg/kg (Group XI), the immobility time was 120 seconds which is similar to normal control animals. 
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Effect of Ashwagandha extract with 80%  
               
               
                 withanolide glycoside with different 
               
               
                 percentage of enteric coating on Immobility time in rats. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Immobility 
               
               
                   
                   
                 time in 
               
               
                 Groups 
                 Treatment 
                 seconds 
               
               
                   
               
               
                 Group I 
                 Normal control (Received only vehicle;  
                 120 
               
               
                   
                 1% Tween 80 v/v p.o.). 
                   
               
               
                 Group II 
                 Reserpine (6 mg/kg i.p) + Vehicle  
                 250 
               
               
                   
                 (1% Tween 80 v/v p.o.). 
                   
               
               
                 Group III 
                 Reserpine (6 mg/kg i.p) +  
                 195 
               
               
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 5)with 
                   
               
               
                   
                 0.5% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group IV 
                 Reserpine (6 mg/kg i.p) +  
                 183 
               
               
                   
                 Ashwagandha extract with 80%  
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 5)with 
                   
               
               
                   
                 1% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group V 
                 Reserpine (6 mg/kg i.p) +  
                 173 
               
               
                   
                 Ashwagandha extract with 80%  
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 5) with 
                   
               
               
                   
                 3% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group VI 
                 Reserpine (6 mg/kg i.p) +  
                 160 
               
               
                   
                 Ashwagandha extract with 80%  
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 5) with 
                   
               
               
                   
                 5% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group VII 
                 Reserpine (6 mg/kg i.p) +  
                 145 
               
               
                   
                 Ashwagandha extract with 80%  
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 5) with 
                   
               
               
                   
                 7% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group VIII 
                 Reserpine (6 mg/kg i.p) +  
                 122 
               
               
                   
                 Ashwagandha extract with 80%  
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 5) with 
                   
               
               
                   
                 10% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group IX 
                 Reserpine (6 mg/kg i.p) +  
                 111 
               
               
                   
                 Ashwagandha extract with 80% 
                   
               
               
                   
                 withanolide glycosides (sample 5  
                   
               
               
                   
                 prepared as per example 5) with 
                   
               
               
                   
                 12% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 Group X 
                 Reserpine (6 mg/kg i.p) +  
                 109 
               
               
                   
                 Ashwagandha extract with 80%  
                   
               
               
                   
                 withanolide glycosides(sample 5  
                   
               
               
                   
                 prepared as per example 5) with 
                   
               
               
                   
                 15% enteric coating (Dosage 20 mg/kg p.o). 
                   
               
               
                 GroupXI 
                 Reserpine(6 mg/kg i.p) + Fluoxetine  
                 120 
               
               
                   
                 (10 mg/kg p.o.). 
               
               
                   
               
            
           
         
       
     
     As shown in the Table 4, immobility time for normal control animals was 120 seconds. When reserpine was injected i.p., the animals got depressed and immobility time was increased to 250 seconds (Group II). Oral administration of Ashwagandha extract with 80% withanolide glycosides with 0.5%, 1% and 3% enteric coating (20 mg/kg) after reserpine injection was not much effective and immobility time was recorded as 195, 183 and 173 seconds respectively (Group III to V). Administration of Ashwagandha extract with 80% withanolide glycosides with 5 and 7% enteric coating (20 mg/kg) after reserpine injection was more effective than lower percentage of coatings and immobility time was recorded as 160 and 145 seconds respectively (Group VI to VII). Administration of Ashwagandha extract with 80% withanolide glycosides with 10, 12 and 15% enteric coating (20 mg/kg) after reserpine injection was most effective and immobility time was recorded as 122, 111 and 109 seconds respectively (Group VIII to X). In case of fluoxetine standard at 10 mg/kg (Group XI), the immobility time was 120 seconds which is similar to normal control animals. 
     Example 32 
     Antidiabetic Activity of Ashwagandha Extract in Streptozotocin Induced Diabetic Rats. 
     Different Ashwagandha extracts with and without coating were evaluated for antidiabetic activity in streptozotocin (STZ) induced diabetic rats. Male/Female albino wistar rats were maintained as per standard guidelines: housed in polypropylene cages, under 12 hour artificial light and dark cycles at a temperature of 24±2° C., given a standard pellet diet and water ad libitum. The animals were acclimatized to the animal house conditions for a week before beginning the experiment. 
     Diabetes was induced by injecting streptozotocin 35 mg/kg dissolved in 0.1M citrate buffer of pH 4.5, intra-peritoneally. Five days after induction of diabetes (day 1 of the study), animals were fasted for 12 hours and the fasting blood glucose level (FBG) was estimated for diagnosing diabetic rats. Animals with FBG above 200 mg/dl were considered diabetic. The diabetic animals were randomly divided into 13 groups of six animals each. One normal group was also included comprising of six normal rats. 
     Following table 1 shows the treatment schedule given to the respective group of animals for 28 days. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Treatment schedule. 
               
            
           
           
               
               
               
            
               
                   
                 Groups 
                 Drugs administered 
               
               
                   
                   
               
               
                   
                 Group I 
                 Vehicle for 28 days (Normal control). 
               
               
                   
                 Group II 
                 STZ + Vehicle for 28 days (Untreated control). 
               
               
                   
                 Group III 
                 STZ + Glibenclamide (0.5 mg/kg)  
               
               
                   
                   
                 for 28 days (Standard). 
               
               
                   
                 Group IV 
                 STZ + Regular Ashwagandha extract  
               
               
                   
                   
                 with minimum 1% withanolide 
               
               
                   
                   
                 glycosides (prepared as per example 23)  
               
               
                   
                   
                 (Dosage 100 mg/kg) for 28 
               
               
                   
                   
                 days. 
               
               
                   
                 Group V 
                 STZ + Regular Ashwagandha  
               
               
                   
                   
                 extract with 1% withanolide 
               
               
                   
                   
                 glycosides (prepared as per  
               
               
                   
                   
                 example 23)coated with Eudragit 
               
               
                   
                   
                 (prepared as per example 8)  
               
               
                   
                   
                 (Dosage 20 mg/kg) for 28 days. 
               
               
                   
                 Group VI 
                 STZ + Ashwagandha root extract  
               
               
                   
                   
                 with 5% withanolide glycosides 
               
               
                   
                   
                 (sample 2 prepared as per example 6)  
               
               
                   
                   
                 (100 mg/kg.) for 28 days. 
               
               
                   
                 Group VII 
                 STZ + Ashwagandha root extract  
               
               
                   
                   
                 with 5% withanolide glycosides 
               
               
                   
                   
                 (sample 2 prepared as per  
               
               
                   
                   
                 example 6) coated with Eudragit 
               
               
                   
                   
                 (prepared as per example 8)  
               
               
                   
                   
                 (20 mg/kg.) for 28 days. 
               
               
                   
                 Group VIII 
                 STZ + Ashwagandha extract  
               
               
                   
                   
                 with minimum 3.5% withanolide 
               
               
                   
                   
                 glycosides (sample 2 prepared  
               
               
                   
                   
                 as per example 19) (Dosage 100 
               
               
                   
                   
                 mg/kg) for 28 days. 
               
               
                   
                 Group IX 
                 STZ + Ashwagandha extract with  
               
               
                   
                   
                 minimum 3.5% withanolide 
               
               
                   
                   
                 glycosides (sample 2 prepared as  
               
               
                   
                   
                 per example 19) coated with 
               
               
                   
                   
                 Eudragit (prepared as per example  
               
               
                   
                   
                 20) (Dosage 20 mg/kg ) for 28 
               
               
                   
                   
                 days. 
               
               
                   
                 Group X 
                 STZ + Purified Ashwagandha root  
               
               
                   
                   
                 extract with 35% withanolide 
               
               
                   
                   
                 glycoside (sample 3 prepared as  
               
               
                   
                   
                 per example 4) (Dosage 100 mg/kg) 
               
               
                   
                   
                 for 28 days. 
               
               
                   
                 Group XI 
                 STZ + Purified Ashwagandha  
               
               
                   
                   
                 root extract with 35% withanolide 
               
               
                   
                   
                 glycoside (sample 3 prepared as per  
               
               
                   
                   
                 example 4) coated with Eudragit 
               
               
                   
                   
                 (prepared as per example 8).  
               
               
                   
                   
                 (Dosage 20 mg/kg) for 28 days. 
               
               
                   
                 Group XII 
                 STZ + Purified Ashwagandha root  
               
               
                   
                   
                 extract with 80% withanolide 
               
               
                   
                   
                 glycoside (sample 5 prepared as per  
               
               
                   
                   
                 example 5) (Dosage 100 mg/kg) 
               
               
                   
                   
                 for 28 days. 
               
               
                   
                 Group XIII 
                 STZ + Purified Ashwagandha root  
               
               
                   
                   
                 extract with 80% withanolide 
               
               
                   
                   
                 glycoside (sample 5 prepared  
               
               
                   
                   
                 as per example 5) coated with 
               
               
                   
                   
                 Eudragit (prepared as per example 8).  
               
               
                   
                   
                 (Dosage 20 mg/kg) for 28 
               
               
                   
                   
                 days. 
               
               
                   
                   
               
            
           
         
       
     
     Fasting blood glucose level and body weight of rats was measured initially and then at Day 7, Day 14, Day 21 and Day 28 of the study. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Fasting blood glucose (FBG) level of  
               
               
                 diabetic rats treated with Ashwagandha extract. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fasting Blood Glucose level (mg/dl) 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                 Day 1 
                 Day 
                 Day 
                 Day 
                 Day 
               
               
                 Groups 
                 Treatment 
                 (Initial) 
                 7 
                 14 
                 21 
                 28 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Group I 
                 Vehicle. 
                 74 
                 75 
                 74 
                 75 
                 75 
               
               
                 Group II 
                 Untreated control. 
                 425 
                 440 
                 428 
                 425 
                 422 
               
               
                 Group III 
                 Glibenclamide. 
                 422 
                 380 
                 245 
                 196 
                 124 
               
               
                 Group IV 
                 Ashwagandha extract with 
                 423 
                 401 
                 374 
                 337 
                 302 
               
               
                   
                 minimum 1% withanolide 
                   
                   
                   
                   
                   
               
               
                   
                 glycosides (Dosage 100 mg/kg). 
                   
                   
                   
                   
                   
               
               
                 Group V 
                 Ashwagandha extract with 1% 
                 428 
                 400 
                 375 
                 336 
                 300 
               
               
                   
                 withanolide glycosides coated with 
                   
                   
                   
                   
                   
               
               
                   
                 Eudragit (Dosage 20 mg/kg). 
                   
                   
                   
                   
                   
               
               
                 Group VI 
                 Ashwagandha root extract with 5% 
                 425 
                 395 
                 324 
                 284 
                 224 
               
               
                   
                 withanolide glycosides (100 
                   
                   
                   
                   
                   
               
               
                   
                 mg/kg.). 
                   
                   
                   
                   
                   
               
               
                 Group 
                 Ashwagandha root extract with 5% 
                 423 
                 394 
                 323 
                 282 
                 222 
               
               
                 VII 
                 withanolide glycosides coated with 
                   
                   
                   
                   
                   
               
               
                   
                 Eudragit (20 mg/kg.). 
                   
                   
                   
                   
                   
               
               
                 Group 
                 Ashwagandha extract with 
                 425 
                 320 
                 272 
                 209 
                 163 
               
               
                 VIII 
                 minimum 3.5% withanolide 
                   
                   
                   
                   
                   
               
               
                   
                 glycosides) (Dosage 100 mg/kg). 
                   
                   
                   
                   
                   
               
               
                 Group IX 
                 Ashwagandha extract with 
                 426 
                 318 
                 270 
                 206 
                 162 
               
               
                   
                 minimum 3.5% withanolide 
                   
                   
                   
                   
                   
               
               
                   
                 glycosides coated with Eudragit 
                   
                   
                   
                   
                   
               
               
                   
                 (Dosage 20 mg/kg). 
                   
                   
                   
                   
                   
               
               
                 Group X 
                 Purified Ashwagandha root extract 
                 424 
                 365 
                 240 
                 164 
                 120 
               
               
                   
                 with 35% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 (Dosage 100 mg/kg). 
                   
                   
                   
                   
                   
               
               
                 Group XI 
                 Purified Ashwagandha root extract 
                 424 
                 363 
                 239 
                 163 
                 119 
               
               
                   
                 with 35% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 coated with Eudragit. (Dosage 20 
                   
                   
                   
                   
                   
               
               
                   
                 mg/kg). 
                   
                   
                   
                   
                   
               
               
                 Group 
                 Purified Ashwagandha root extract 
                 426 
                 320 
                 205 
                 131 
                 73 
               
               
                 XII 
                 with 80% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 (Dosage 100 mg/kg). 
                   
                   
                   
                   
                   
               
               
                 Group 
                 Purified Ashwagandha root extract 
                 425 
                 318 
                 202 
                 128 
                 72 
               
               
                 XIII 
                 with 80% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 coated with Eudragit (Dosage 20 
                   
                   
                   
                   
                   
               
               
                   
                 mg/kg). 
               
               
                   
               
            
           
         
       
     
     The diabetes was successfully induced in rats after STZ injection. The FBG of Group I rats (vehicle control) remained normal till end of the study. The FBG of untreated control group (Group II) remained high (above 400 mg/dl) during 28 days study period. The standard drug Glibenclamide (Group III) was effective in reducing the FBG level and it reduced from 422 to 124 mg/dl in 28 days treatment. Treatment with Ashwagandha extract with minimum 1% withanolide glycosides at 100 mg/kg dose (Group IV) reduced the FBG from 423 to 302 mg/dl whereas the same extract after coating with Eudragit (Group V) reduced the FBG from 428 to 300 mg/dl. Ashwagandha root extract with 5% withanolide glycosides (Group VI) at 100 mg/kg reduced the FBG from 425 to 224 mg/dl whereas coated product (Group VII) reduced the level from 423 to 222 mg/dl. Ashwagandha extract with minimum 3.5% withanolide glycosides (Group VIII) at 100 mg/kg and its coated product (Group IX) at 20 mg/kg dose reduced the FBG from 425 to 163 and 426 to 162 mg/dl respectively. The purified Ashwagandha root extract with 35% withanolide glycoside (Group X) given at 100 mg/kg reduced the FBG level from 424 to 120 mg/dl whereas purified Ashwagandha root extract with 35% withanolide glycoside coated with Eudragit (Group XI) given at 20 mg/kg for 28 days reduced the FBG from 424 to 119 mg/dl. Purified Ashwagandha root extract with 80% withanolide glycoside (Group XII) at 100 mg/kg and its coated product (Group XIII) at 20 mg/kg were most effective and reduced the FBG level from 426 to 73 and 425 to 72 mg/dl respectively. 
     Example 33 
     Antidiabetic Activity of Coated Ashwagandha Extracts in Different Doses in Streptozotocin Induced Diabetic Rats. 
     Different Ashwagandha extracts with coating in different dosages was evaluated for antidiabetic activity in experimental rats. Male/Female albino wistar rats were maintained as per standard guidelines: housed in polypropylene cages, under 12 hour artificial light and dark cycles at a temperature of 24±2° C., given a standard pellet diet and water ad libitum. The animals were acclimatized to the animal house conditions for a week before beginning the experiment. 
     Diabetes was induced by injecting streptozotocin 35 mg/kg dissolved in 0.1M citrate buffer of pH 4.5, intra-peritoneally. Five days after induction of diabetes (day 1 of the study), animals were fasted for 12 hours and the fasting blood glucose level (FBG) was estimated for diagnosing diabetic rats. Animals with FBG above 200 mg/dl were considered diabetic. The diabetic animals were randomly divided into 13 groups of 4 animals each. 
     Following table shows the treatment schedule given to the respective group of animals for 28 days. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Treatment schedule 
               
            
           
           
               
               
               
            
               
                   
                 Groups 
                 Drugs administered 
               
               
                   
                   
               
               
                   
                 Group I 
                 Vehicle for 28 days (Normal control). 
               
               
                   
                 Group II 
                 STZ + Vehicle for 28 days (Untreated control). 
               
               
                   
                 Group III 
                 STZ + Glibenclamide (0.5 mg/kg)  
               
               
                   
                   
                 for 28 days (Standard). 
               
               
                   
                 Group IV 
                 Ashwagandha extract with minimum  
               
               
                   
                   
                 3.5% withanolide glycosides 
               
               
                   
                   
                 (sample 2 prepared as per example19)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 20) (Dosage 1 mg/kg) for 28 days. 
               
               
                   
                 Group V 
                 Ashwagandha extract with 3.5%  
               
               
                   
                   
                 withanolide glycosides (sample 2 
               
               
                   
                   
                 prepared as per example 19 ) coated  
               
               
                   
                   
                 with Eudragit (prepared as per 
               
               
                   
                   
                 example 20) (Dosage 5 mg/kg) for 28 days. 
               
               
                   
                 Group VI 
                 Ashwagandha extract with minimum  
               
               
                   
                   
                 3.5% withanolide glycosides 
               
               
                   
                   
                 (sample 2 prepared as per example19)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 20) (Dosage 10 mg/kg) for 28 days. 
               
               
                   
                 Group VII 
                 Ashwagandha extract with minimum  
               
               
                   
                   
                 3.5% withanolide glycosides 
               
               
                   
                   
                 (sample 2 prepared as per example 19)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 20) (Dosage 20 mg/kg) for 28 days 
               
               
                   
                 Group VIII 
                 Ashwagandha extract with minimum  
               
               
                   
                   
                 3.5% withanolide glycosides 
               
               
                   
                   
                 (sample 2 prepared as per example 19)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 20) (Dosage 40 mg/kg) for 28 days. 
               
               
                   
                 Group IX 
                 Purified Ashwagandha root extract  
               
               
                   
                   
                 with 35% withanolide glycoside 
               
               
                   
                   
                 (sample 3 prepared as per example 4)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 8). (Dosage 1 mg/kg) for 28 days. 
               
               
                   
                 Group X 
                 Purified Ashwagandha root extract  
               
               
                   
                   
                 with 35% withanolide glycoside 
               
               
                   
                   
                 (sample 3 prepared as per example 4)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 8). (Dosage 5 mg/kg) for 28 days. 
               
               
                   
                 Group XI 
                 Purified Ashwagandha root extract  
               
               
                   
                   
                 with 35% withanolide glycoside 
               
               
                   
                   
                 (sample 3 prepared as per example 4)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 8). (Dosage 10 mg/kg) for 28 days. 
               
               
                   
                 Group XII 
                 Purified Ashwagandha root extract  
               
               
                   
                   
                 with 35% withanolide glycoside 
               
               
                   
                   
                 (sample 3 prepared as per example 4)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 8). (Dosage 20 mg/kg) for 28 days. 
               
               
                   
                 Group XIII 
                 Purified Ashwagandha root extract  
               
               
                   
                   
                 with 35% withanolide glycoside 
               
               
                   
                   
                 (sample 3 prepared as per example 4)  
               
               
                   
                   
                 coated with Eudragit (prepared as 
               
               
                   
                   
                 per example 8). (Dosage 40 mg/kg) for 28 days. 
               
               
                   
                   
               
            
           
         
       
     
     Fasting blood glucose level and body weight of rats was measured initially and then at Day 7, Day 14, Day 21 and Day 28 of the study. 
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Fasting blood glucose (FBG) level of diabetic rats treated with  
               
               
                 Ashwagandha extract in different doses. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Fasting Blood Glucose level (mg/dl) 
               
            
           
           
               
               
               
               
               
               
               
            
               
                   
                   
                 Day 1 
                 Day 
                 Day 
                 Day 
                 Day 
               
               
                 Groups 
                 Treatment 
                 (Initial) 
                 7 
                 14 
                 21 
                 28 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
            
               
                 Group I 
                 Vehicle. 
                 73 
                 74 
                 73 
                 74 
                 74 
               
               
                 Group II 
                 Untreated control. 
                 426 
                 441 
                 429 
                 426 
                 423 
               
               
                 Group III 
                 Glibenclamide. 
                 423 
                 381 
                 246 
                 197 
                 125 
               
               
                 Group IV 
                 Ashwagandha extract with 3.5% 
                 426 
                 422 
                 391 
                 345 
                 301 
               
               
                   
                 withanolide glycosides coated 
                   
                   
                   
                   
                   
               
               
                   
                 with Eudragit (Dosage 1 mg/kg) 
                   
                   
                   
                   
                   
               
               
                   
                 for 28 days. 
                   
                   
                   
                   
                   
               
               
                 Group V 
                 Ashwagandha extract with 3.5% 
                 425 
                 418 
                 350 
                 282 
                 250 
               
               
                   
                 withanolide glycosides coated with 
                   
                   
                   
                   
                   
               
               
                   
                 Eudragit (Dosage 5 mg/kg) for 28 
                   
                   
                   
                   
                   
               
               
                   
                 days. 
                   
                   
                   
                   
                   
               
               
                 Group VI 
                 Ashwagandha extract with 3.5% 
                 426 
                 386 
                 318 
                 274 
                 202 
               
               
                   
                 withanolide glycosides coated 
                   
                   
                   
                   
                   
               
               
                   
                 with Eudragit (Dosage 10 mg/kg) 
                   
                   
                   
                   
                   
               
               
                   
                 for 28 days. 
                   
                   
                   
                   
                   
               
               
                 Group VII 
                 Ashwagandha extract with 3.5% 
                 425 
                 322 
                 276 
                 212 
                 164 
               
               
                   
                 withanolide glycosides coated 
                   
                   
                   
                   
                   
               
               
                   
                 with Eudragit (Dosage 20 mg/kg) 
                   
                   
                   
                   
                   
               
               
                   
                 for 28 days. 
                   
                   
                   
                   
                   
               
               
                 Group VIII 
                 Ashwagandha extract with 3.5% 
                 428 
                 301 
                 265 
                 192 
                 145 
               
               
                   
                 withanolide glycosides coated with 
                   
                   
                   
                   
                   
               
               
                   
                 Eudragit (Dosage 40 mg/kg) for 
                   
                   
                   
                   
                   
               
               
                   
                 28 days. 
                   
                   
                   
                   
                   
               
               
                 Group IX 
                 Purified Ashwagandha root extract 
                 426 
                 420 
                 352 
                 284 
                 250 
               
               
                   
                 with 35% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 coated with Eudragit (Dosage 1 
                   
                   
                   
                   
                   
               
               
                   
                 mg/kg) for 28 days. 
                   
                   
                   
                   
                   
               
               
                 Group X 
                 Purified Ashwagandha root extract 
                 425 
                 410 
                 318 
                 241 
                 200 
               
               
                   
                 with 35% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 coated with Eudragit (Dosage 5 
                   
                   
                   
                   
                   
               
               
                   
                 mg/kg) for 28 days. 
                   
                   
                   
                   
                   
               
               
                 Group XI 
                 Purified Ashwagandha root extract 
                 423 
                 400 
                 280 
                 201 
                 160 
               
               
                   
                 with 35% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 coated with Eudragit (Dosage 10 
                   
                   
                   
                   
                   
               
               
                   
                 mg/kg) for 28 days. 
                   
                   
                   
                   
                   
               
               
                 Group XII 
                 Purified Ashwagandha root extract 
                 424 
                 361 
                 242 
                 169 
                 120 
               
               
                   
                 with 35% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 coated with Eudragit. (Dosage 20 
                   
                   
                   
                   
                   
               
               
                   
                 mg/kg) for 28 days. 
                   
                   
                   
                   
                   
               
               
                 Group XIII 
                 Purified Ashwagandha root extract 
                 425 
                 332 
                 215 
                 126 
                 80 
               
               
                   
                 with 35% withanolide glycoside 
                   
                   
                   
                   
                   
               
               
                   
                 coated with Eudragit (Dosage 40 
                   
                   
                   
                   
                   
               
               
                   
                 mg/kg) for 28 days. 
               
               
                   
               
            
           
         
       
     
     The FBG of Group I rats (vehicle control) remained normal till end of the study. The FBG of untreated control group (Group II) remained high (above 400 mg/dl) during 28 days study period. The standard drug Glibenclamide (Group III) was very effective in reducing the FBG level and it reduced from 423 to 125 mg/dl in 28 days treatment. Ashwagandha extract with 3.5% withanolide glycosides coated with Eudragit (Group IV to VIII) at 1, 5, 10, 20 and 40 mg/kg daily dosage for 28 days reduced the FBG level to 301, 250, 202, 164 and 145 mg/dl respectively. Purified Ashwagandha root extract with 35% withanolide glycoside coated with Eudragit was the most effective in reducing the FBG levels in rats. This product at 1, 5, 10, 20 and 40 mg/kg daily dosage (Group IX to XIII) reduced the FBG level to 250, 200, 160, 120 and 80 mg/dl respectively. 
     Example 34 
     Endurance Study. 
     Forced swimming test (FST) in rats was used to evaluate swimming endurance. The test included two exposures to a water tank (height, 40 cm; diameter, 22 cm, containing 25 cm of water at 25° C.) spaced 24 h apart. The first exposure was 10 min long (training) and the second, serving as the test session, was 5 min long. Each animal made vigorous attempts to get out of water bath during first couple of minutes and thereafter surrendered to experimental conditions and assumed a typical immobile posture (which is defined as when no additional activity is observed other than that required to keep the head above the water) with occasional escape attempts. The total duration of immobility was recorded. 
     24 male rats were divided into 4 groups comprising of 6 rats in each group. Experiment was performed as per above procedure and immobility time was recorded. 
     
       
         
           
               
               
               
             
               
                   
               
               
                   
                   
                 Immobility 
               
               
                 Groups 
                 Treatment 
                 time (Sec) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 Group I 
                 Control. 
                 118.67 
               
               
                 Group II 
                 Ashwagandha extract with 3.5%  
                 77.33 
               
               
                   
                 withanolide glycosides coated 
                   
               
               
                   
                 with Eudragit prepared as per  
                   
               
               
                   
                 example 20(Dosage 20 mg/kg). 
                   
               
               
                 Group III 
                 Amaranth extract with 9%  
                 66.67 
               
               
                   
                 nitrate content prepared as per 
                   
               
               
                   
                 example 27(Dosage 50 mg/kg). 
                   
               
               
                 Group IV 
                 Ashwagandha extract with 3.5%  
                 52.67 
               
               
                   
                 withanolide glycosides coated 
                   
               
               
                   
                 with Eudragit + Amaranth extract  
                   
               
               
                   
                 with 9% nitrate content 
                   
               
               
                   
                 blended in 1:1 ratio prepared as  
                   
               
               
                   
                 per example 28 (Dosage50 
                   
               
               
                   
                 mg/kg). 
               
               
                   
               
            
           
         
       
     
     For control group (Group I), the average immobility time for rats in 5 minutes test session was 118.67 seconds whereas after feeding with Ashwagandha extract with 3.5% withanolide glycosides coated with Eudragit (Group II) at 20 mg/kg increased the swimming endurance and immobility time got reduced to 77.33 seconds. The immobility time in rats fed with Amaranth extract with 9% nitrate content (Group III) at 50 mg/kg was recorded as 66.67 seconds. Feeding of rats with a combination of Ashwagandha extract with 3.5% withanolide glycosides coated with Eudragit and Amaranth extract with 9% nitrate content was most effective and it reduced the immobility time to 52.67 seconds. 
     Other modifications and variations to the invention will be apparent to those skilled in the art from the foregoing disclosure and teachings. Thus, while only certain embodiments of the invention have been specifically described herein, it will be apparent that numerous modifications may be made thereto without departing from the spirit and scope of the invention.