Patent Publication Number: US-2022213196-A1

Title: Antigen-binding proteins targeting shared antigens

Description:
CROSS REFERENCE 
     This application claims the benefit of U.S. Provisional Application No. 62/719,565, filed Aug. 17, 2018, U.S. Provisional Application No. 62/808,775, filed Feb. 21, 2019, and U.S. Provisional Application No. 62/869,923, filed Jul. 2, 2019, which applications are hereby incorporated by reference in their entirety. 
    
    
     RELATED APPLICATIONS 
     This application is related to PCT/US2018/046997, filed on Aug. 17, 2018, and to PCT/US2018/06793, filed on Dec. 28, 2018, which applications are incorporated by reference in their entirety. 
     SEQUENCE LISTING 
     [insert] 
     BACKGROUND 
     The immune system employs two types of adaptive immune responses to provide antigen specific protection from pathogens; humoral immune responses, and cellular immune responses, which involve specific recognition of pathogen antigens via B lymphocytes and T lymphocytes, respectively. 
     T lymphocytes, by virtue of being the antigen specific effectors of cellular immunity, play a central role in the body&#39;s defense against diseases mediated by intracellular pathogens, such as viruses, intracellular bacteria, mycoplasmas, and intracellular parasites, and against cancer cells by directly cytolysing the affected cells. The specificity of T lymphocyte responses is conferred by, and activated through T-cell receptors (TCRs) binding to (major histocompatibility complex) MHC molecules on the surface of affected cells. T-cell receptors are antigen specific receptors clonally distributed on individual T lymphocytes whose repertoire of antigenic specificity is generated via somatic gene rearrangement mechanisms analogous to those involved in generating the antibody gene repertoire. T-cell receptors include a heterodimer of transmembrane molecules, the main type being composed of an alpha-beta polypeptide dimer and a smaller subset of a gamma-delta polypeptide dimer. T lymphocyte receptor subunits comprise a variable and constant region similar to immunoglobulins in the extracellular domain, a short hinge region with cysteine that promotes alpha and beta chain pairing, a transmembrane and a short cytoplasmic region. Signal transduction triggered by TCRs is indirectly mediated via CD3-zeta, an associated multi-subunit complex comprising signal transducing subunits. 
     T lymphocyte receptors do not generally recognize native antigens but rather recognize cell-surface displayed complexes comprising an intracellularly processed fragment of an antigen in association with a major histocompatibility complex (MHC) for presentation of peptide antigens. Major histocompatibility complex genes are highly polymorphic across species populations, comprising multiple common alleles for each individual gene. In humans, MHC is referred to as human leukocyte antigen (HLA). 
     Major histocompatibility complex class I molecules are expressed on the surface of virtually all nucleated cells in the body and are dimeric molecules comprising a transmembrane heavy chain, comprising the peptide antigen binding cleft, and a smaller extracellular chain termed beta2-microglobulin. MHC class I molecules present peptides derived from the degradation of cytosolic proteins by the proteasome, a multi-unit structure in the cytoplasm, (Niedermann G., 2002. Curr Top Microbiol Immunol. 268:91-136; for processing of bacterial antigens, refer to Wick M J, and Ljunggren H G., 1999. Immunol Rev. 172:153-62). Cleaved peptides are transported into the lumen of the endoplasmic reticulum (ER) by the transporter associated with antigen processing (TAP) where they are bound to the groove of the assembled class I molecule, and the resultant MHC/peptide complex is transported to the cell membrane to enable antigen presentation to T lymphocytes (Yewdell J W., 2001. Trends Cell Biol. 11:294-7; Yewdell J W. and Bennink J R., 2001. Curr Opin Immunol. 13:13-8). Alternatively, cleaved peptides can be loaded onto MHC class I molecules in a TAP-independent manner and can also present extracellularly-derived proteins through a process of cross-presentation. As such, a given MHC/peptide complex presents a novel protein structure on the cell surface that can be targeted by a novel antigen-binding protein (e.g., antibodies or TCRs) once the identity of the complex&#39;s structure (peptide sequence and MHC subtype) is determined. 
     Tumor cells can express antigens and may display such antigens on the surface of the tumor cell. Such tumor-associated antigens can be used for development of novel immunotherapeutic reagents for the specific targeting of tumor cells. For example, tumor-associated antigens can be used to identify therapeutic antigen binding proteins, e.g., TCRs, antibodies, or antigen-binding fragments. Such tumor-associated antigens may also be utilized in pharmaceutical compositions, e.g., vaccines. 
     SUMMARY 
     Provided herein is an isolated antigen binding protein (ABP) that specifically binds to a human leukocyte antigen (HLA)-PEPTIDE target, wherein the HLA-PEPTIDE target comprises an HLA-restricted peptide complexed with an HLA Class I molecule, wherein the HLA-restricted peptide is located in the peptide binding groove of an α1/α2 heterodimer portion of the HLA Class I molecule, wherein the HLA Class I molecule is HLA subtype B*35:01 (reference sequence: MGSHSMRYFYTAMSRPGRGEPRFIAVGYVDDTQFVRFDSDAASPRTEPRAPWIEQE GPEYWDRNTQIFKTNTQTYRESLRNLRGYYNQSEAGSHIIQRMYGCDLGPDGRLLR GHDQSAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAARVAEQLRAYLEGLCVE WLRRYLENGKETLQRADPPKTHVTHHPVSDHEATLRCWALGFYPAEITLTWQRDGE DQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLR) and the HLA-restricted peptide comprises the sequence EVDPIGHVY, and wherein the ABP binds to any one or more of: (a) any one or more of amino acid positions 2-9 of the restricted peptide EVDPIGHVY; (b) any one or more of amino acid positions 50, 54, 55, 57, 61, 62, 74, 81, 82 and 85 of the α1 helix of HLA subtype B*35:01; and (c) any one or more of amino acid positions 147 and 148 of the α2 helix of HLA subtype B*35:01. Note that recited ranges include terminal residues. For example, an ABP that binds to any one or more of positions 2-9 of the restricted peptide EVDPIGHVY contacts at least one of residues 2, 3, 4, 5, 6, 7, 8, and 9 of the restricted peptide EVDPIGHVY. 
     In some embodiments, the ABP binds to any one or more of amino acid positions 2-8 of the restricted peptide EVDPIGHVY 
     In some embodiments, the ABP binds to any one or more of amino acid positions 5-9 of the restricted peptide EVDPIGHVY. 
     In some embodiments, the HLA Class I molecule is HLA subtype B*35:01 and the HLA-restricted peptide consists of the sequence EVDPIGHVY 
     In some embodiments, the ABP comprises a CDR-H3 comprising a sequence selected from: CARDGVRYYGMDVW, CARGVRGYDRSAGYW, CASHDYGDYGEYFQHW, CARVSWYCSSTSCGVNWFDPW, CAKVNWNDGPYFDYW, CATPTNSGYYGPYYYYGMDVW, CARDVMDVW, CAREGYGMDVW, CARDNGVGVDYW, CARGIADSGSYYGNGRDYYYGMDVW, CARGDYYFDYW, CARDGTRYYGMDVW, CARDVVANFDYW, CARGHSSGWYYYYGMDVW, CAKDLGSYGGYYW, CARSWFGGFNYHYYGMDVW, CARELPIGYGMDVW, and CARGGSYYYYGMDVW. 
     In some embodiments, the ABP comprises a CDR-L3 comprising a sequence selected from: CMQGLQTPITF, CMQALQTPPTF, CQQAISFPLTF, CQQANSFPLTF, CQQANSFPLTF, CQQSYSIPLTF, CQQTYMMPYTF, CQQSYITPWTF, CQQSYITPYTF, CQQYYTTPYTF, CQQSYSTPLTF, CMQALQTPLTF, CQQYGSWPRTF, CQQSYSTPVTF, CMQALQTPYTF, CQQANSFPFTF, CMQALQTPLTF, and CQQSYSTPLTF. 
     In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G5_P7_E7, G5_P7_B3, G5_P7_A5, G5_P7_F6, G5-P1B12, G5-P1C12, G5-P1-E05, G5-P3G01, G5-P3G08, G5-P4B02, G5-P4E04, G5R4-P1D06, G5R4-P1H11, G5R4-P2B10, G5R4-P2H8, G5R4-P3G05, G5R4-P4A07, or G5R4-P4B01. 
     In some embodiments, the ABP comprises all three heavy chain CDRs and all three light chain CDRs from the scFv designated G5_P7_E7, G5_P7_B3, G5_P7_A5, G5_P7_F6, G5-P1B12, G5-P1C12, G5-P1-E05, G5-P3G01, G5-P3G08, G5-P4B02, G5-P4E04, G5R4-P1D06, G5R4-P1H11, G5R4-P2B10, G5R4-P2H8, G5R4-P3G05, G5R4-P4A07, or G5R4-P4B01. 
     In some embodiments, the ABP comprises a VH sequence selected from 
     
       
         
           
               
            
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYDINWVRQAPGQGLEWMGI 
               
               
                   
               
               
                 INPRSGSTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDG 
               
               
                   
               
               
                 VRYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGYTFTSHDINWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 MNPNSGDTGYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARGV 
               
               
                   
               
               
                 RGYDRSAGYWGQGTLVIVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVKPGGSLRLSCAASGFSFSSYWMSWVRQAPGKGLEWISY 
               
               
                   
               
               
                 ISGDSGYTNYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCASHD 
               
               
                   
               
               
                 YGDYGEYFQHWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLQSGGGLVQPGGSLRLSCAASGFTFSNSDMNWVRQAPGKGLEWVAY 
               
               
                   
               
               
                 ISSGSSTIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARVS 
               
               
                   
               
               
                 WYCSSTSCGVNWFDPWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFSNSDMNWVRQAPGKGLEWVAS 
               
               
                   
               
               
                 ISSSGGYINYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKVN 
               
               
                   
               
               
                 WNDGPYFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGGTFSNFGVSWLRQAPGQGLEWMGG 
               
               
                   
               
               
                 IIPILGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCATPT 
               
               
                   
               
               
                 NSGYYGPYYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYNMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDV 
               
               
                   
               
               
                 MDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSGYLVSWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPNSGGTNTAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREG 
               
               
                   
               
               
                 YGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYIFRNYPMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPDSGGTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDN 
               
               
                   
               
               
                 GVGVDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 MNPNIGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGI 
               
               
                   
               
               
                 ADSGSYYGNGRDYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYGISWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPNSGVTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGD 
               
               
                   
               
               
                 YYFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYDINWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPNSGDTKYSQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDG 
               
               
                   
               
               
                 TRYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVKPGGSLRLSCAASGFTFSDYYMSWVRQAPGKGLEWVSY 
               
               
                   
               
               
                 ISSSSSYTNYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCARDV 
               
               
                   
               
               
                 VANFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 MNPDSGSTGYAQRFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGH 
               
               
                   
               
               
                 SSGWYYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFTSYSMHWVRQAPGKGLEWVSS 
               
               
                   
               
               
                 ITSFTNTMYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKDL 
               
               
                   
               
               
                 GSYGGYYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYMHWVRQAPGQGLEWMGI 
               
               
                   
               
               
                 INPSGGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARSW 
               
               
                   
               
               
                 FGGFNYHYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 MNPNSGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREL 
               
               
                   
               
               
                 PIGYGMDVWGQGTTVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGG 
               
               
                   
               
               
                 IIPIVGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARGG 
               
               
                   
               
               
                 SYYYYGMDVWGQGTTVTVSS. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises a VL sequence selected from 
     
       
         
           
               
            
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSYRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGLQTP 
               
               
                   
               
               
                 ITFGQGTRLEIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSSRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 PTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQAISFPLTFGQ 
               
               
                   
               
               
                 STKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYS 
               
               
                   
               
               
                 ASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSIPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLNWYQQKPGKAPKLLIYY 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQTYMMPYTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKWYGAS 
               
               
                   
               
               
                 SLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYITPWTFGQGT 
               
               
                   
               
               
                 KVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYITPYTFGQ 
               
               
                   
               
               
                 GTKLEIK, 
               
               
                   
               
               
                 DIVMTQSPDSLAVSLGERATINCKTSQSVLYRPNNENYLAWYQQKPGQPP 
               
               
                   
               
               
                 KLLIYQASIREPGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYTT 
               
               
                   
               
               
                 PYTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRFLNWYQQKPGKAPKLLIYG 
               
               
                   
               
               
                 ASRPQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSHRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 LTFGGGTKVEIK, 
               
               
                   
               
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVSSNLAWYQQKPGQAPRLLIYA 
               
               
                   
               
               
                 ASARASGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYGSWPRTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKWYGAS 
               
               
                   
               
               
                 RLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPVTFGQGT 
               
               
                   
               
               
                 KVEIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 YTFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASEDISNHLNWYQQKPGKAPKWYDAL 
               
               
                   
               
               
                 SLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPFTFGPGT 
               
               
                   
               
               
                 KVDIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 LTFGQGTKVEIK, 
               
               
                 and 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGG 
               
               
                   
               
               
                 GTKVEIK. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises the VH sequence and VL sequence from the scFv designated G5_P7_E7, G5_P7_B3, G5_P7_A5, G5_P7_F6, G5-P1B12, G5-P1C12, G5-P1-E05, G5-P3G01, G5-P3G08, G5-P4B02, G5-P4E04, G5R4-P1D06, G5R4-P1H11, G5R4-P2B10, G5R4-P2H8, G5R4-P3G05, G5R4-P4A07, and G5R4-P4B01. 
     Also provided herein is an isolated antigen binding protein (ABP) that specifically binds to a human leukocyte antigen (HLA)-PEPTIDE target, wherein the HLA-PEPTIDE target comprises an HLA-restricted peptide complexed with an HLA Class I molecule, wherein the HLA-restricted peptide is located in the peptide binding groove of an α1/α2 heterodimer portion of the HLA Class I molecule, the HLA Class I molecule is HLA subtype A*01:01 (reference sequence: MGSHSMRYFF TSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEG PEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLR GYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCV DGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGE DQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLR), and the HLA-restricted peptide comprises the sequence NTDNNLAVY, and wherein the ABP binds to any one or more of: (a) any one or more of residues 3-9 of the restricted peptide NTDNNLAVY, (b) any one or more of residues 70-85 of the of the alpha 1 helix of HLA subtype allele A*01:01, and (c) any one or more of residues 140-160 of the alpha 2 helix of HLA subtype allele A*01:01. 
     In some embodiments, the ABP binds to any one or more of residues 6-9 of the restricted peptide NTDNNLAVY 
     In some embodiments, the ABP binds to any one or more of residues 7-8 of the restricted peptide NTDNNLAVY 
     In some embodiments, the ABP binds to one or more of residues 157-160 of the alpha 2 helix of HLA subtype allele A*01:01. 
     In some embodiments, the ABP binds to one or more of residues 6-9 of the restricted peptide NTDNNLAVY and one or more of residues 157-160 of the alpha 2 helix of the HLA subtype allele A*01:01. 
     In some embodiments, the HLA Class I molecule is HLA subtype A*01:01 and the HLA-restricted peptide consists of the sequence NTDNNLAVY 
     In some embodiments, the ABP comprises a CDR-H3 comprising a sequence selected from: CAATEWLGVW, CARANWLDYW, CARANWLDYW, CARDWVLDYW, CARGEWLDYW, CARGWELGYW, CARDFVGYDDW, CARDYGDLDYW, CARGSYGMDVW, CARDGYSGLDVW, CARDSGVGMDVW, CARDGVAVASDYW, CARGVNVDDFDYW, CARGDYTGNWYFDLW, CARANWLDYW, CARDQFYGGNSGGHDYW, CAREEDYW, CARGDWFDPW, CARGDWFDPW, CARGEWFDPW, CARSDWFDPW, CARDSGSYFDYW, CARDYGGYVDYW, CAREGPAALDVW, CARERRSGMDVW, CARVLQEGMDVW, CASERELPFDIW, CAKGGGGYGMDVW, CAAMGIAVAGGMDVW, CARNWNLDYW, CATYDDGMDVW, CARGGGGALDYW, CALSGNYYGMDVW, CARGNPWELRLDYW, and CARDKNYYGMDVW. 
     In some embodiments, the ABP comprises a CDR-L3 comprising a sequence selected from: CQQSYNTPYTF, CQQSYSTPYTF, CQQSYSTPYSF, CQQSYSTPFTF, CQQSYGVPYTF, CQQSYSAPYTF, CQQSYSAPYTF, CQQSYSAPYSF, CQQSYSTPYTF, CQQSYSVPYSF, CQQSYSAPYTF, CQQSYSVPYSF, CQQSYSTPQTF, CQQLDSYPFTF, CQQSYSSPYTF, CQQSYSTPLTF, CQQSYSTPYSF, CQQSYSTPYTF, CQQSYSTPYTF, CQQSYSTPFTF, CQQSYSTPTF, CQQTYAIPLTF, CQQSYSTPYTF, CQQSYIAPFTF, CQQSYSIPLTF, CQQSYSNPTF, CQQSYSTPYSF, CQQSYSDQWTF, CQQSYLPPYSF, CQQSYSSPYTF, CQQSYTTPWTF, CQQSYLPPYSF, CQEGITYTF, CQQYYSYPFTF, and CQHYGYSPVTF. 
     In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G2-P1H11, G2-P2E07, G2-P2E03, G2-P2A11, G2-P2C06, G2-P1G01, G2-P1C02, G2-P1H01, G2-P1B12, G2-P1B06, G2-P2H10, G2-P1H10, G2-P2C11, G2-P1C09, G2-P1A10, G2-P1B10, G2-P1D07, G2-P1E05, G2-P1D03, G2-P1G12, G2-P2H11, G2-P1C03, G2-P1G07, G2-P1F12, G2-P1G03, G2-P2B08, G2-P2A10, G2-P2D04, G2-P1C06, G2-P2A09, G2-P1B08, G2-P1E03, G2-P2A03, G2-P2F01, or G2-P1D06. 
     In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G2-P1H11. 
     In some embodiments, the ABP comprises all three heavy chain CDRs and all three light chain CDRs from the scFv designated G2-P1H11, G2-P2E07, G2-P2E03, G2-P2A11, G2-P2C06, G2-P1G01, G2-P1C02, G2-P1H01, G2-P1B12, G2-P1B06, G2-P2H10, G2-P1H10, G2-P2C11, G2-P1C09, G2-P1A10, G2-P1B10, G2-P1D07, G2-P1E05, G2-P1D03, G2-P1G12, G2-P2H11, G2-P1C03, G2-P1G07, G2-P1F12, G2-P1G03, G2-P2B08, G2-P2A10, G2-P2D04, G2-P1C06, G2-P2A09, G2-P1B08, G2-P1E03, G2-P2A03, G2-P2F01, or G2-P1D06. 
     In some embodiments, the ABP comprises all three heavy chain CDRs and all three light chain CDRs from the scFv designated G2-P1H11. 
     In some embodiments, the ABP comprises a VH sequence selected from 
     
       
         
           
               
            
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYMEIWVRQAPGQGLEWM 
               
               
                   
               
               
                 GMINPSGGGTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCA 
               
               
                   
               
               
                 RGNPWELRLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSATISWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WIYPNSGGTVYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAA 
               
               
                   
               
               
                 TEWLGVWGQGTTVTVSS, 
               
               
                   
               
               
                 EVQLLQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTISAPNFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 ANWLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGAEVKKPGASVKVSCKASGYTFTTYDLAWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 ANWLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKSSGYSFDSYVVNWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DWVLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WMNPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GEWLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GWELGYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTRYTINWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DFVGYDDWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGITWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DYGDLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSNYILSWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPDSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GSYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYSFTRYNMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DGYSGLDVWGKGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYAISWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNNGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DSGVGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFNNYAFSWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DGVAVASDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSSYNMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINGNTGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GVNVDDFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYAFSWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPDTGYTRYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GDYTGNWYFDLWGRGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPYSGGTNYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 ANWLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WISAYNGYTNYAQNLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DQFYGGNSGGHDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYNMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WMNPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 E-EDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTRYTINWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGANYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GDWFDPWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYLMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WISPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GDWFDPWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSDYYVHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GEWFDPWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTTYYMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 SDWFDPWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSNYAINWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WISPYSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DSGSYFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYYMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WIYPNTGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DYGGYVDYWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGAEVKKPGASVKVSCKASGYTFTSYAMNWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WMNPNSGGTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 EGPAALDVWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTLTSHLIHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 ERRSGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 EVQLLESGAEVKKPGASVKVSCKASGYSFTDYIVHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPYSGGTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 VLQEGMDVWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSNFLINWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAS 
               
               
                   
               
               
                 ERELPFDIWGQGTMVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYQMFWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAK 
               
               
                   
               
               
                 GGGGYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYAISWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAA 
               
               
                   
               
               
                 MGIAVAGGMDVWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYHMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WIHPDSGGTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 NWNLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WMNPNSGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAT 
               
               
                   
               
               
                 YDDGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYTVNWVRQAPGQGLEWMG 
               
               
                   
               
               
                 WINPNSGGTKYAQNFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 GGGGALDWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 MINPRDDTTDYARDFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAL 
               
               
                   
               
               
                 SGNYYGMDVWGQGTTVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGYTFTSQYMHWVRQAPGQGLEWMG 
               
               
                   
               
               
                 RIIPLLGIVNYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAR 
               
               
                   
               
               
                 DKNYYGMDVWGQGTTVTVSS. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises a VL sequence selected from 
     
       
         
           
               
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSG 
                   
               
               
                   
               
               
                 TDFTLTISSLQPEDFATYYCQQYYSYPFTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISTWLAWYQQKPGKAPKLLIYAASSLRSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYNTPYTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIYAASTVQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQDISRWLAWYQQKPGKAPKLLIYAASRLQAGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYSFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQTISSWLAWYQQKPGKAPKLLIYAASSLQSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQTISSWLAWYQQKPGKAPKLLIYAASSLQSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYGVPYTFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISNWLAWYQQKPGKAPKLLIYAASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSVGNWLAWYQQKPGKAPKLLIYGASSLQTGVP 
               
               
                   
               
               
                 SRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQNIGNWLAWYQQKPGKAPKLLIYAASTLQTGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYSFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIYAASSLQSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYGASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSVPYSFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISKWLAWYQQKPGKAPKLLIYAASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIYAASTLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSVPYSFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQTISNYLNWYQQKPGKAPKLLIYAASNLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPQTFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASRDIGRAVGWYQQKPGKAPKLLIYAASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQLDSYPFTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYAASTLQSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYSSPYTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYAASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGGGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSIGRWLAWYQQKPGKAPKLLIYAASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYSFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISTWLAWYQQKPGKAPKLLIYAASTLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTFAQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYGASRLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYAASTLQSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSVSNWLAWYQQKPGKAPKLLIYAASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYAASTLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQTYAIPLTFGGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDIGSWLAWYQQKPGKAPKLLIYATSSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISRWLAWYQQKPGKAPKLLIYAASTLQPGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYIAPFTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIYAASRLESGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSIPLTFGGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKWYGVSSLQSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYSNPTFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWVAWYQQKPGKAPKLLIYGASNLESGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYSFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIYAASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSDQWTFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIYAASSLQSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYLPPYSFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISNWLAWYQQKPGKAPKLLIYAASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTYFTLTISSLQPEDFATYYCQQSYSSPYTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISHYLNWYQQKPGKAPKLLIYGASSLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTTPWTFGQGTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYAASTLQSGVPSR 
               
               
                   
               
               
                 FSGSGSGTDFTLTISSLQPEDFATYYCQQSYLPPYSFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYGASRLQSGVPS 
               
               
                   
               
               
                 RFSGSGSGTDFTLTISSLQPEDFATYYCQEGITYTFGQGTKVEIK, 
               
               
                 and 
               
               
                   
               
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVSRNLAWYQQKPGQAPRLLIYGASTRATGIPAR 
               
               
                   
               
               
                 FSGSGSGTEFTLTISSLQSEDFAVYYCQHYGYSPVTFGQGTKLEIK. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises the VH sequence and the VL sequence from the scFv designated G2-P1H11, G2-P2E07, G2-P2E03, G2-P2A11, G2-P2C06, G2-P1G01, G2-P1C02, G2-P1H01, G2-P1B12, G2-P1B06, G2-P2H10, G2-P1H10, G2-P2C11, G2-P1C09, G2-P1A10, G2-P1B10, G2-P1D07, G2-P1E05, G2-P1D03, G2-P1G12, G2-P2H11, G2-P1C03, G2-P1G07, G2-P1F12, G2-P1G03, G2-P2B08, G2-P2A10, G2-P2D04, G2-P1C06, G2-P2A09, G2-P1B08, G2-P1E03, G2-P2A03, G2-P2F01, or G2-P1D06. 
     In some embodiments, the ABP comprises the VH sequence and the VL sequence from the scFv designated G2-P1H11. 
     Also provided herein is an isolated antigen binding protein (ABP) that specifically binds to a human leukocyte antigen (HLA)-PEPTIDE target, wherein the HLA-PEPTIDE target comprises an HLA-restricted peptide complexed with an HLA Class I molecule, wherein the HLA-restricted peptide is located in the peptide binding groove of an α1/α2 heterodimer portion of the HLA Class I molecule, wherein the HLA Class I molecule is HLA subtype A*02:01 (reference sequence: 
                    MGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVREDSDAASQRMEPRA               PWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMY               GCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEA               AHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEA               TLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVV               PSGQEQRYTCHVQHEGLPKPLTLR),            
and the HLA-restricted peptide comprises the sequence AIFPGAVPAA, and wherein the ABP binds to any one or more of: (a) any one or more of amino acid positions 1-6 of the restricted peptide AIFPGAVPAA, (b) any one or more of amino acid positions 46, 49, 55, 61, 74, 76, 77, 78, 81 and 84 of the α1 helix of HLA subtype A*02:01, (c) any one or more of amino acid positions 45-60, 66, 67, and 73 of the α1 helix of HLA subtype A*02:01, (d) any one or more of amino acid positions 138, 145, 147, 152-156, 164, 167 of the α2 helix of HLA subtype A*02:01, and (e) any one or more of any one or more of amino acid positions 56, 59, 60, 63, 64, 66, 67, 70, 73, 74, 132, 150-153, 155, 156, 158-160, 162-164, 166-168, 170, and 171 of HLA subtype A*02:01.
 
     In some embodiments, the ABP binds to any one or more of amino acid positions 1-5 of the restricted peptide AIFPGAVPAA. 
     In some embodiments, the ABP binds to one or both of amino acid positions 4 and 5 of the restricted peptide AIFPGAVPAA. 
     In some embodiments, the ABP binds to one or both of amino acid positions 5 and 6 of the restricted peptide AIFPGAVPAA. 
     In some embodiments, the ABP binds to amino acid position 6 of the restricted peptide AIFPGAVPAA. 
     In some embodiments, the ABP binds to any one or more of amino acid positions 46, 49, 55, 66, 67, and 73 of the α1 helix of HLA subtype A*02:01. 
     In some embodiments, the ABP comprises a VH region comprising a paratope comprising at least one, two, three, or four of residues Tyr32, Gly99, Asp100, and Tyr100A of the VH region shown in the sequence QVQLVQSGAEVKKPGASVKVSCKASGGTLSSYPINWVRQAPGQGLEWMGWISTYS GHADYAQKLQGRVTMTRDTSTSTVYMEL SSLRSEDTAVYYCARSYDYGDYLNFDY WGQGTLVTVSS, as numbered by the Kabat numbering system. 
     In some embodiments, the ABP comprises a VH region comprising a paratope comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22 of residues Thr28, Leu 29, Ser 30, Ser 31, Tyr 32, Pro 33, Trp 47, Trp 50, Ser 52, Tyr 53, Ser 54, His 56, Asp 58, Tyr 59, Gln 61, Gln 64, Asp 97, Tyr 98, Gly 99, Asp100, Tyr100A, Leu100B, and Asn100C of the VH region shown in the sequence QVQLVQSGAEVKKPGASVKVSCKASGGTLSSYPINWVRQAPGQGLEWMGWISTYS GHADYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARSYDYGDYLNFDY WGQGTLVTVSS, as numbered by the Kabat numbering system. 
     In some embodiments, the paratope comprises at least 1, 2, 3, 4, 5, 6, or 7 of residues Ser 30, Ser 31, Tyr 32, Tyr 98, Gly 99, Asp 100, and Tyr 100A of the VH region, as numbered by the Kabat numbering system. 
     In some embodiments, the ABP comprises a VL region comprising a paratope comprising at least one, two, or three of residues Tyr32, Ser 91, and Tyr 92 of the VL region shown in the sequence DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYAASSLQSGV PSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSIPPTFGGGTKVDIK, as numbered by the Kabat numbering system. 
     In some embodiments, the ABP comprises a VL region comprising a paratope comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13 of residues Asp1, Ser30, Asn31, Tyr32, Tyr49, Ala50, Ser53, Ser67, Ser91, Tyr92, Ser93, Ile94, and Pro95 of the VL region shown in the sequence DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYAASSLQSGV PSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSIPPTFGGGTKVDIK, as numbered by the Kabat numbering system. 
     In some embodiments, the paratope comprises at least 1, 2, 3, 4, 5, or 6 of residues Asp1, Asn31, Tyr32, Ser91, Tyr92, and Ile94 of the VL region, as numbered by the Kabat numbering system. 
     In some embodiments, the HLA Class I molecule is HLA subtype A*02:01 and the HLA-restricted peptide consists of the sequence AIFPGAVPAA. 
     In some embodiments, the ABP comprises a CDR-H3 comprising a sequence selected from: CARDDYGDYVAYFQHW, CARDLSYYYGMDVW, CARVYDFWSVLSGFDIW, CARVEQGYDIYYYYYMDVW, CARSYDYGDYLNFDYW, CARASGSGYYYYYGMDVW, CAASTWIQPFDYW, CASNGNYYGSGSYYNYW, CARAVYYDFWSGPFDYW, CAKGGIYYGSGSYPSW, CARGLYYMDVW, CARGLYGDYFLYYGMDVW, CARGLLGFGEFLTYGMDVW, CARDRDSSWTYYYYGMDVW, CARGLYGDYFLYYGMDVW, CARGDYYDSSGYYFPVYFDYW, and CAKDPFWSGHYYYYGMDVW. 
     In some embodiments, the ABP comprises a CDR-L3 comprising a sequence selected from: CQQNYNSVTF, CQQSYNTPWTF, CGQSYSTPPTF, CQQSYSAPYTF, CQQSYSIPPTF, CQQSYSAPYTF, CQQHNSYPPTF, CQQYSTYPITI, CQQANSFPWTF, CQQSHSTPQTF, CQQSYSTPLTF, CQQSYSTPLTF, CQQTYSTPWTF, CQQYGSSPYTF, CQQSHSTPLTF, CQQANGFPLTF, and CQQSYSTPLTF. 
     In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G8-P1A03, G8-P1A04, G8-P1A06, G8-P1B03, G8-P1C11, G8-P1D02, G8-P1H08, G8-P2B05, G8-P2E06, R3G8-P2C10, R3G8-P2E04, R3G8-P4F05, R3G8-P5C03, R3G8-P5F02, R3G8-P5G08, G8-P1C01, or G8-P2C11. 
     In some embodiments, the ABP comprises all three heavy chain CDRs and all three light chain CDRs from the scFv designated G8-P1A03, G8-P1A04, G8-P1A06, G8-P1B03, G8-P1C11, G8-P1D02, G8-P1H08, G8-P2B05, G8-P2E06, R3G8-P2C10, R3G8-P2E04, R3G8-P4F05, R3G8-P5C03, R3G8-P5F02, R3G8-P5G08, G8-P1C01, or G8-P2C11. 
     In some embodiments, the ABP comprises a VH sequence selected from: 
     
       
         
           
               
               
            
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSRSAITWVRQAPGQGLEWMGWINPNSGAT 
                   
               
               
                   
               
               
                 NYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDDYGDYVAYFQHWGQGT 
               
               
                   
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYPFIGQYLHWVRQAPGQGLEWMGIINPSGDSA 
               
               
                   
               
               
                 TYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDLSYYYGMDVWGQGTTV 
               
               
                   
               
               
                 TVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYMHWVRQAPGQGLEWMGWMNPIG 
               
               
                   
               
               
                 GGTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARVYDFWSVLSGFDIWG 
               
               
                   
               
               
                 QGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFSDYYMSWVRQAPGKGLEWVSGINWNGGST 
               
               
                   
               
               
                 GYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARVEQGYDIYYYYYMDVWG 
               
               
                   
               
               
                 KGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTLSSYPINWVRQAPGQGLEWMGWISTYSGH 
               
               
                   
               
               
                 ADYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARSYDYGDYLNFDYWGQG 
               
               
                   
               
               
                 TLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVSSISGRGDNT 
               
               
                   
               
               
                 YYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARASGSGYYYYYGMDVWGQ 
               
               
                   
               
               
                 GTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFGNYFMHWVRQAPGQGLEWMGMVNPSGG 
               
               
                   
               
               
                 SETFAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAASTWIQPFDYWGQGTLVT 
               
               
                   
               
               
                 VSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFDFSIYSMNWVRQAPGKGLEWVSAISGSGGSTY 
               
               
                   
               
               
                 YADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCASNGNYYGSGSYYNYWGQGTL 
               
               
                   
               
               
                 VTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTLTTYYMHWVRQAPGQGLEWMGWINPNSG 
               
               
                   
               
               
                 GTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARAVYYDFWSGPFDYWGQ 
               
               
                   
               
               
                 GTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGWINPYSG 
               
               
                   
               
               
                 GTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAKGGIYYGSGSYPSWGQG 
               
               
                   
               
               
                 TLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYGVSWVRQAPGQGLEWMGWISPYSGN 
               
               
                   
               
               
                 TDYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARGLYYMDVWGKGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSNMYLHWVRQAPGQGLEWMGWINPNTG 
               
               
                   
               
               
                 DTNYAQTFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGLYGDYFLYYGMDVW 
               
               
                   
               
               
                 GQGTKVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGWMNPNS 
               
               
                   
               
               
                 GNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGLLGFGEFLTYGMDV 
               
               
                   
               
               
                 WGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYIHWVRQAPGQGLEWMGVINPSGGS 
               
               
                   
               
               
                 TTYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDRDSSWTYYYYGMDVW 
               
               
                   
               
               
                 GQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSNYMHWVRQAPGQGLEWMGWMNPNS 
               
               
                   
               
               
                 GNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGLYGDYFLYYGMDV 
               
               
                   
               
               
                 WGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSHAISWVRQAPGQGLEWMGVIIPSGGTS 
               
               
                   
               
               
                 YTQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGDYYDSSGYYFPVYFDYWG 
               
               
                   
               
               
                 QGTLVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYAMNWVRQAPGQGLEWMGWINPNSG 
               
               
                   
               
               
                 GTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDPFWSGHYYYYGMDV 
               
               
                   
               
               
                 WGQGTTVTVSS. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises a VL sequence selected from: 
     
       
         
           
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSITSYLNWYQQKPGKAPKWYDAS 
               
               
                   
               
               
                 NLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQNYNSVTFGQGTK 
               
               
                   
               
               
                 LEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCWASQGISSYLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYNTPWTFGP 
               
               
                   
               
               
                 GTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQAISNSLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCGQSYSTPPTFGQ 
               
               
                   
               
               
                 GTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYK 
               
               
                   
               
               
                 ASSLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTFGP 
               
               
                   
               
               
                 GTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSIPPTFGG 
               
               
                   
               
               
                 GTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGINSYLAWYQQKPGKAPKWYDAS 
               
               
                   
               
               
                 NLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHNSYPPTFGQGT 
               
               
                   
               
               
                 KLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYSTYPITIGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNSLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPWTFGQ 
               
               
                   
               
               
                 GTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQDVSTWLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSHSTPQTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYD 
               
               
                   
               
               
                 ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGG 
               
               
                   
               
               
                 GTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNWLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQTYSTPWTFGQ 
               
               
                   
               
               
                 GTKLEIK, 
               
               
                   
               
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVGNSLAWYQQKPGQAPRLLIYG 
               
               
                   
               
               
                 ASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYGSSPYTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISGYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSHSTPLTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQNIYTYLNWYQQKPGKAPKLLIYD 
               
               
                   
               
               
                 ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANGFPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                 and 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGG 
               
               
                   
               
               
                 GTKVEIK. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises the VH sequence and VL sequence from the scFv designated G8-P1A03, G8-P1A04, G8-P1A06, G8-P1B03, G8-P1C11, G8-P1D02, G8-P1H08, G8-P2B05, G8-P2E06, R3G8-P2C10, R3G8-P2E04, R3G8-P4F05, R3G8-P5C03, R3G8-P5F02, R3G8-P5G08, G8-P1C01, or G8-P2C11. 
     Also provided herein is an isolated antigen binding protein (ABP) that specifically binds to a human leukocyte antigen (HLA)-PEPTIDE target, wherein the HLA-PEPTIDE target comprises an HLA-restricted peptide complexed with an HLA Class I molecule, wherein the HLA-restricted peptide is located in the peptide binding groove of an α1/α2 heterodimer portion of the HLA Class I molecule, wherein the HLA Class I molecule is HLA subtype A*01:01 (reference sequence: MGSHSMRYFF TSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEG PEYWDQETRNMKAHSQTDRANLGTLRGYYNQ SEDGSHTIQIMYGCDVGPDGRFLR GYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCV DGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGE DQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLR), and the HLA-restricted peptide comprises the sequence ASSLPTTMNY, and wherein the ABP binds to any one or more of: (a) any one or more of amino acid positions 4, 6, 7, 8, and 9 of the restricted peptide ASSLPTTMNY, (b) any one or more of amino acid positions 49-56 of HLA subtype A*01:01, (c) any one or more of amino acid positions 59-66 of HLA subtype A*01:01, (d) any one or more of amino acid positions 136-147 of HLA subtype A*01:01, and (e) any one or more of amino acid positions 157-160 of HLA subtype A*01:01. 
     In some embodiments, the ABP binds to any one or more of amino acid positions 6-9 of the restricted peptide ASSLPTTMNY 
     In some embodiments, the ABP binds to any one or more of amino acid positions 6-7 of the restricted peptide ASSLPTTMNY 
     In some embodiments, the ABP binds to amino acid positions 6 of the restricted peptide ASSLPTTMNY. 
     In some embodiments, the ABP binds to: (a) any one or more of amino acid positions 52-54 of HLA subtype A*01:01, (b) any one or more of amino acid positions 136-139 of HLA subtype A*01:01, (c) any one or more of amino acid positions 141-147 of HLA subtype A*01:01, or (d) any one or more of amino acid positions 136-139 and any one or more of amino acid positions 141-147 of HLA subtype A*01:01. 
     In some embodiments of the ABP comprising an antibody or antigen-binding fragment thereof, the HLA Class I molecule is HLA subtype A*01:01 and the HLA-restricted peptide consists of the sequence ASSLPTTMNY 
     In some embodiments, the ABP comprises a CDR-H3 comprising a sequence selected from: CARDQDTIFGVVITWFDPW, CARDKVYGDGFDPW, CAREDDSMDVW, CARDSSGLDPW, CARGVGNLDYW, CARDAHQYYDFWSGYYSGTYYYGMDVW, CAREQWPSYWYFDLW, CARDRGYSYGYFDYW, CARGSGDPNYYYYYGLDVW, CARDTGDHFDYW, CARAENGMDVW, CARDPGGYMDVW, CARDGDAFDIW, CARDMGDAFDIW, CAREEDGMDVW, CARDTGDHFDYW, CARGEYSSGFFFVGWFDLW, and CARETGDDAFDIW. 
     In some embodiments, the ABP comprises a CDR-L3 comprising a sequence selected from: CQQYFTTPYTF, CQQAEAFPYTF, CQQSYSTPITF, CQQSYIIPYTF, CHQTYSTPLTF, CQQAYSFPWTF, CQQGYSTPLTF, CQQANSFPRTF, CQQANSLPYTF, CQQSYSTPFTF, CQQSYSTPFTF, CQQSYGVPTF, CQQSYSTPLTF, CQQSYSTPLTF, CQQYYSYPWTF, CQQSYSTPFTF, CMQTLKTPLSF, and CQQSYSTPLTF. 
     In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated R3G10-P1A07, R3G10-P1B07, R3G10-P1E12, R3G10-P1F06, R3G10-P1H01, R3G10-P1H08, R3G10-P2C04, R3G10-P2G11, R3G10-P3E04, R3G10-P4A02, R3G10-P4C05, R3G10-P4D04, R3G10-P4D10, R3G10-P4E07, R3G10-P4E12, R3G10-P4G06, R3G10-P5A08, or R3G10-P5C08. 
     In some embodiments, the ABP comprises all three heavy chain CDRs and all three light chain CDRs from the scFv designated R3G10-P1A07, R3G10-P1B07, R3G10-P1E12, R3G10-P1F06, R3G10-P1H01, R3G10-P1H08, R3G10-P2C04, R3G10-P2G11, R3G10-P3E04, R3G10-P4A02, R3G10-P4C05, R3G10-P4D04, R3G10-P4D10, R3G10-P4E07, R3G10-P4E12, R3G10-P4G06, R3G10-P5A08, or R3G10-P5C08. 
     In some embodiments, the ABP comprises a VH sequence selected from: 
     
       
         
           
               
               
            
               
                 EVQLLESGGGLVKPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVSGISARSGRT 
                   
               
               
                   
               
               
                 YYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCARDQDTIFGVVITWFDPWGQG 
               
               
                   
               
               
                 TLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGIIHPGGGT 
               
               
                   
               
               
                 TSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDKVYGDGFDPWGQGTLV 
               
               
                   
               
               
                 TVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYIFTGYYMHWVRQAPGQGLEWMGMIGPSDGS 
               
               
                   
               
               
                 TSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREDDSMDVWGKGTTVTVS 
               
               
                   
               
               
                 S, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFIGYYMHWVRQAPGQGLEWMGMIGPSDGS 
               
               
                   
               
               
                 TSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDSSGLDPWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGMIGPSDG 
               
               
                   
               
               
                 STSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGVGNLDYWGQGTLVTV 
               
               
                   
               
               
                 SS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGVTFSTSAISWVRQAPGQGLEWMGWISPYNGNT 
               
               
                   
               
               
                 DYAQMLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDAHQYYDFWSGYYSGTY 
               
               
                   
               
               
                 YYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSNSIINWVRQAPGQGLEWMGWMNPNSGN 
               
               
                   
               
               
                 TNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREQWPSYWYFDLWGRGTL 
               
               
                   
               
               
                 VTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSTHDINWVRQAPGQGLEWMGVINPSGGS 
               
               
                   
               
               
                 AIYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDRGYSYGYFDYWGQGTL 
               
               
                   
               
               
                 VTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGNTFIGYYVHWVRQAPGQGLEWVGIINPNGGSI 
               
               
                   
               
               
                 SYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGSGDPNYYYYYGLDVWG 
               
               
                   
               
               
                 QGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTLSYYYMHWVRQAPGQGLEWMGMIGPSDG 
               
               
                   
               
               
                 STSYAQRFQGRVTMTRDTSTGTVYMELSSLRSEDTAVYYCARDTGDHFDYWGQGTLVT 
               
               
                   
               
               
                 VSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGIIGPSDGS 
               
               
                   
               
               
                 TTYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARAENGMDVWGQGTTVTV 
               
               
                   
               
               
                 SS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYVHWVRQAPGQGLEWMGIIAPSDGS 
               
               
                   
               
               
                 TNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDPGGYMDVWGKGTTVT 
               
               
                   
               
               
                 VSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYLHWVRQAPGQGLEWMGMIGPSDGS 
               
               
                   
               
               
                 TSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDGDAFDIWGQGTMVTVS 
               
               
                   
               
               
                 S, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGRISPSDGS 
               
               
                   
               
               
                 TTYAPKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARDMGDAFDIWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGMIGPSDG 
               
               
                   
               
               
                 STSYAQRFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREEDGMDVWGQGTTVTV 
               
               
                   
               
               
                 SS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTLSYYYMEIWVRQAPGQGLEWMGMIGPSDG 
               
               
                   
               
               
                 STSYAQRFQGRVTMTRDTSTGTVYMELSSLRSEDTAVYYCARDTGDHFDYWGQGTLVT 
               
               
                   
               
               
                 VSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGGTFNNFAISWVRQAPGQGLEWMGGIIPIFDATN 
               
               
                   
               
               
                 YAQKFQGRVTFTADESTSTAYMELSSLRSEDTAVYYCARGEYSSGFFFVGWFDLWGRGT 
               
               
                   
               
               
                 QVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYNFTGYYMEIWVRQAPGQGLEWMGIIAPSDGS 
               
               
                   
               
               
                 TNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARETGDDAFDIWGQGTMVT 
               
               
                   
               
               
                 VSS.  
               
            
           
         
       
     
     In some embodiments, the ABP comprises a VL sequence selected: 
     
       
         
           
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQGGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYFTTPYTFGQ 
               
               
                   
               
               
                 GTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIFD 
               
               
                   
               
               
                 ASRLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQAEAFPYTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPITFGQ 
               
               
                   
               
               
                 GTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISNYLNWYQQKPGKAPKLLIYK 
               
               
                   
               
               
                 ASSLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYIIPYTFGQ 
               
               
                   
               
               
                 GTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISNYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQTYSTPLTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKWYSAS 
               
               
                   
               
               
                 NLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQAYSFPWTFGQGT 
               
               
                   
               
               
                 KVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQNISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGYSTPLTFGQ 
               
               
                   
               
               
                 GTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQDISRYLAWYQQKPGKAPKLLIYD 
               
               
                   
               
               
                 ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPRTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSLPYTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASTLQNGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTFGP 
               
               
                   
               
               
                 GTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQRISSYLNWYQQKPGKAPKWYSAS 
               
               
                   
               
               
                 TLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTFGPGT 
               
               
                   
               
               
                 KVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLAWYQQKPGKAPKLLIYD 
               
               
                   
               
               
                 ASKLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYGVPTFGQG 
               
               
                   
               
               
                 TKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYD 
               
               
                   
               
               
                 ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISTYLAWYQQKPGKAPKLLIYD 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYSYPWTFGQ 
               
               
                   
               
               
                 GTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASTLQNGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTFGP 
               
               
                   
               
               
                 GTKVDIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQTLKTP 
               
               
                   
               
               
                 LSFGGGTKVEIK, 
               
               
                 and 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGG 
               
               
                   
               
               
                 GTKVEIK. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises the VH sequence and VL sequence from the scFv designated R3G10-P1A07, R3G10-P1B07, R3G10-P1E12, R3G10-P1F06, R3G10-P1H01, R3G10-P1H08, R3G10-P2C04, R3G10-P2G11, R3G10-P3E04, R3G10-P4A02, R3G10-P4C05, R3G10-P4D04, R3G10-P4D10, R3G10-P4E07, R3G10-P4E12, R3G10-P4G06, R3G10-P5A08, or R3G10-P5C08. 
     Also provided herein is an isolated antigen binding protein (ABP) that specifically binds to a human leukocyte antigen (HLA)-PEPTIDE target, wherein the HLA-PEPTIDE target comprises an HLA-restricted peptide complexed with an HLA Class I molecule, wherein the HLA-restricted peptide is located in the peptide binding groove of an α1/α2 heterodimer portion of the HLA Class I molecule, wherein the HLA Class I molecule is HLA subtype A*02:01 (reference sequence: 
                    MGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVREDSDAASQRMEPRA               PWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMY               GCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEA               AHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEA               TLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVV               PSGQEQRYTCHVQHEGLPKPLTLR),            
and the HLA-restricted peptide comprises the sequence LLASSILCA, and wherein the ABP binds to any one or more of: (a) any one or more of residues 1-5 of the restricted peptide LLASSILCA, (b) any one or more of residues 49-85 of the HLA-A*02:01 alpha 1 helix, and (c) any one or more of residues 57-67 of the HLA-A*02:01 alpha 1 helix.
 
     In some embodiments of the ABP comprising an antibody or antigen-binding fragment thereof, the HLA Class I molecule is HLA subtype A*02:01 and the HLA-restricted peptide consists of the sequence LLASSILCA. 
     In some embodiments, the ABP comprises a CDR-H3 comprising a sequence selected from: CARDGYDFWSGYTSDDYW, CASDYGDYR, CARDLMTTVVTPGDYGMDVW, CARQDGGAFAFDIW, CARELGYYYGMDVW, CARALIFGVPLLPYGMDVW, CAKDLATVGEPYYYYGMDVW, and CARLWFGELHYYYYYGMDVW. 
     In some embodiments, the ABP comprises a CDR-L3 comprising a sequence selected from: CHHYGRSHTF, CQQANAFPPTF, CQQYYSIPLTF, CQQSYSTPPTF, CQQSYSFPYTF, CMQALQTPLTF, CQQGNTFPLTF, and CMQGSHWPPSF. 
     In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G7R3-P1C6, G7R3-P1G10, 1-G7R3-P1B4, 2-G7R4-P2C2, 3-G7R4-P1A3, 4-G7R4-B5-P2E9, 5-G7R4-B10-P1F8, or B7 (G7R3-P3A9). 
     In some embodiments, the ABP comprises all three heavy chain CDRs and all three light chain CDRs from the scFv designated G7R3-P1C6, G7R3-P1G10, 1-G7R3-P1B4, 2-G7R4-P2C2, 3-G7R4-P1A3, 4-G7R4-B5-P2E9, 5-G7R4-B10-P1F8, or B7 (G7R3-P3A9). 
     In some embodiments, the ABP comprises a VH sequence selected from 
     
       
         
           
               
            
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSNYGISWVRQAPGQGLEWMGI 
               
               
                   
               
               
                 INPGGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDGY 
               
               
                   
               
               
                 DFWSGYTSDDYWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMHWVRQAPGKGLEWVSG 
               
               
                   
               
               
                 ISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCASDY 
               
               
                   
               
               
                 GDYRGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSNYYIHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 LNPNSGNTGYAQRFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDL 
               
               
                   
               
               
                 MTTVVTPGDYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASMKVSCKASGYTFTTDGISWVRQAPGQGLEWMGR 
               
               
                   
               
               
                 IYPHSGYTEYAKKFKGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARQD 
               
               
                   
               
               
                 GGAFAFDIWGQGTMVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSQYMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 ISPNNGDTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREL 
               
               
                   
               
               
                 GYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASRYTFTSYDINWVRQAPGQGLEWMGR 
               
               
                   
               
               
                 IIPMLNIANYAPKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARAL 
               
               
                   
               
               
                 IFGVPLLPYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 EVQLLQSGGGLVQPGGSLRLSCAASGFTFSSSWMHWVRQAPGKGLEWVSF 
               
               
                   
               
               
                 ISTSSGYIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKDL 
               
               
                   
               
               
                 ATVGEPYYYYGMDVWGQGTTVTVSS, 
               
               
                 and 
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGDTFNTYALSWVRQAPGQGLEWMGW 
               
               
                 MNPNSGNAGYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARLW 
               
               
                 FGELHYYYYYGMDVWGQGTMVTVSS. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises a VL sequence selected from 
     
       
         
           
               
            
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVSSSNLAWYQQKPGQAPRLLIY 
               
               
                   
               
               
                 GASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCHHYGRSHTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQDIRNDLGWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANAFPPTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIVMTQSPDSLAVSLGERATINCKSSQSVFYSSNNKNQLAWYQQKPGQPP 
               
               
                   
               
               
                 KWYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSIPL 
               
               
                   
               
               
                 TFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDIFKYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPPTFGQ 
               
               
                   
               
               
                 GTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISTWLAWYQQKPGKAPKLLIYY 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSFPYTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCSSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 LTFGGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYS 
               
               
                   
               
               
                 ASNLRSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGNTFPLTFGQ 
               
               
                   
               
               
                 GTKVEIK,  
               
               
                 and 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGSHWP 
               
               
                   
               
               
                 PSFGQGTRLEIK. 
               
            
           
         
       
     
     In some embodiments, the ABP comprises the VH sequence and the VL sequence from the scFv designated G7R3-P1C6, G7R3-P1G10, 1-G7R3-P1B4, 2-G7R4-P2C2, 3-G7R4-P1A3, 4-G7R4-B5-P2E9, 5-G7R4-B10-P1F8, or B7 (G7R3-P3A9). 
     In some embodiments, the ABP comprises an antibody or antigen-binding fragment thereof. In some embodiments, the antigen binding protein is linked to a scaffold, optionally the scaffold comprises serum albumin or Fc, optionally wherein Fc is human Fc and is an IgG (IgG1, IgG2, IgG3, IgG4), an IgA (IgA1, IgA2), an IgD, an IgE, or an IgM isotype Fc. In some embodiments, the antigen binding protein is linked to a scaffold via a linker, optionally the linker is a peptide linker, optionally the peptide linker is a hinge region of a human antibody. In some embodiments, the antigen binding protein comprises an Fv fragment, a Fab fragment, a F(ab′)2 fragment, a Fab′ fragment, an scFv fragment, an scFv-Fc fragment, and/or a single-domain antibody or antigen binding fragment thereof. In some embodiments, the antigen binding protein comprises an scFv fragment. In some embodiments, the antigen binding protein comprises one or more antibody complementarity determining regions (CDRs), optionally six antibody CDRs. In some embodiments, the antigen binding protein comprises an antibody. In some embodiments, the antigen binding protein is a monoclonal antibody. In some embodiments, the antigen binding protein is a humanized, human, or chimeric antibody. In some embodiments, the antigen binding protein is multispecific, optionally bispecific. In some embodiments, the antigen binding protein binds greater than one antigen or greater than one epitope on a single antigen. In some embodiments, the antigen binding protein comprises a heavy chain constant region of a class selected from IgG, IgA, IgD, IgE, and IgM. In some embodiments, the antigen binding protein comprises a heavy chain constant region of the class human IgG and a subclass selected from IgG1, IgG4, IgG2, and IgG3. In some embodiments, the antigen binding protein comprises one or more modifications that extend half-life. In some embodiments, the antigen binding protein comprises a modified Fc, optionally the modified Fc comprises one or more mutations that extend half-life, optionally the one or more mutations that extend half-life is YTE. 
     In some embodiments of the isolated ABP, the ABP comprises a T cell receptor (TCR) or an antigen-binding portion thereof. In some embodiments, the TCR or antigen-binding portion thereof comprises a TCR variable region. In some embodiments, the TCR or antigen-binding portion thereof comprises one or more TCR complementarity determining regions (CDRs). 
     In some embodiments, the TCR comprises an alpha chain and a beta chain. In some embodiments, the TCR comprises a gamma chain and a delta chain. 
     In some embodiments, the antigen binding protein is a portion of a chimeric antigen receptor (CAR) comprising: an extracellular portion comprising the antigen binding protein; and an intracellular signaling domain. In some embodiments, the antigen binding protein comprises an scFv and the intracellular signaling domain comprises an immunoreceptor tyrosine-based activation motif (ITAM). In some embodiments, the intracellular signaling domain comprises a signaling domain of a zeta chain of a CD3-zeta (CD3) chain. 
     In some embodiments, the ABP further comprises a transmembrane domain linking the extracellular domain and the intracellular signaling domain. In some embodiments, the transmembrane domain comprises a transmembrane portion of CD28. 
     In some embodiments, the ABP further comprises an intracellular signaling domain of a T cell costimulatory molecule. In some embodiments, the T cell costimulatory molecule is CD28, 4-1BB, OX-40, ICOS, or any combination thereof. 
     Also provided herein is an isolated polynucleotide encoding an isolated ABP as described herein. 
     In some embodiments of the ABP, the antigen binding protein binds to the HLA-PEPTIDE target through a contact point with the HLA Class I molecule and through a contact point with the HLA-restricted peptide of the HLA-PEPTIDE target. In some embodiments of the ABP, the binding of the ABP to the amino acid positions on the restricted peptide or HLA subtype, or the contact points or residues that impact binding, directly or indirectly, of the HLA-PEPTIDE target with the ABP are determined via positional scanning, hydrogen-deuterium exchange, or protein crystallography. 
     In some embodiments, the ABP may be for use as a medicament. In some embodiments, the ABP may be for use in treatment of cancer, optionally wherein the cancer expresses or is predicted to express the HLA-PEPTIDE target. In some embodiments, the ABP may be for use in treatment of cancer, wherein the cancer is selected from a solid tumor and a hematological tumor. 
     Also provided herein is an ABP which is a conservatively modified variant of the ABP as described herein. Also provided herein is an antigen binding protein (ABP) that competes for binding with the antigen binding protein as described herein. Also provided herein is an antigen binding protein (ABP) that binds the same HLA-PEPTIDE epitope bound by the antigen binding protein as described herein. 
     Also provided herein is an engineered cell expressing a receptor comprising the antigen binding protein as described herein. In some embodiments, the engineered cell is a T cell, optionally a cytotoxic T cell (CTL). In some embodiments of the engineered cell, the antigen binding protein is expressed from a heterologous promoter. 
     Also provided herein is an isolated polynucleotide or set of polynucleotides encoding the antigen binding protein described herein or an antigen-binding portion thereof. 
     Also provided herein is an isolated polynucleotide or set of polynucleotides encoding the HLA/peptide targets described herein. 
     Also provided herein is a vector or set of vectors comprising the polynucleotide or set of polynucleotides described herein. 
     Also provided herein is a host cell comprising the polynucleotide or set of polynucleotides a described herein, or the vector or set of vectors described herein, optionally wherein the host cell is CHO or HEK293, or optionally wherein the host cell is a T cell. 
     Also provided herein is a method of producing an antigen binding protein comprising expressing the antigen binding protein with the host cell described herein and isolating the expressed antigen binding protein. 
     Also provided herein is a pharmaceutical composition comprising the antigen binding protein as described herein and a pharmaceutically acceptable excipient. 
     Also provided herein is a method of treating cancer in a subject, comprising administering to the subject an effective amount of the antigen binding protein as described herein or a pharmaceutical composition described herein, optionally wherein the cancer is selected from a solid tumor and a hematological tumor. In some embodiments, the cancer expresses or is predicted to express the HLA-PEPTIDE target. 
     Also provided herein is a kit comprising the antigen binding protein described herein or a pharmaceutical composition described herein and instructions for use. 
     Also provided herein is a composition comprising at least one HLA-PEPTIDE target described herein and an adjuvant. 
     Also provided herein is a composition comprising at least one HLA-PEPTIDE target described herein and a pharmaceutically acceptable excipient. 
     Also provided herein is a composition comprising an amino acid sequence comprising a polypeptide of at least one HLA-PEPTIDE target disclosed in Table A, Table A1, or Table A2, optionally the amino acid sequence consisting essentially of or consisting of the polypeptide. 
     Also provided herein is a virus comprising the isolated polynucleotide or set of polynucleotides as described herein. In some embodiments, the virus is a filamentous phage. 
     Also provided herein is a yeast cell comprising the isolated polynucleotide or set of polynucleotides as described herein. 
    
    
     
       BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS 
       These and other features, aspects, and advantages of the present invention will become better understood with regard to the following description, and accompanying drawings, where: 
         FIG. 1  shows the general structure of a Human Leukocyte Antigen (HLA) Class I molecule. By User atropos235 on en.wikipedia—Own work, CC BY 2.5, https://commons.wikimedia.org/w/index.php?curid=1805424 
         FIG. 2  depicts exemplary construct elements for cloning TCRs into expression systems for therapy development. 
         FIG. 3  shows the target and minipool negative control design for HLA-PEPTIDE target “G5”. 
         FIG. 4  shows the target and minipool negative control design for HLA-PEPTIDE targets “G8” and “G10”. 
         FIGS. 5A and 5B  show HLA stability results for the G5 counterscreen “minipool” and G5 target. 
         FIGS. 6A-6E  show HLA stability results for the G5 “complete” pool counterscreen peptides. 
         FIGS. 7A and 7B  show HLA stability results for counterscreen peptides and G8 target. 
         FIGS. 8A and 8B  show HLA stability results for the G10 counterscreen “minipool” and G10 target. 
         FIGS. 9A-9D  show HLA stability results for the additional G8 and G10 “complete” pool counterscreen peptides. 
         FIGS. 10A-10C  show phage supernatant ELISA results, indicating progressive enrichment of G5-, G8 and G10 binding phage with successive panning rounds. 
         FIG. 11  shows a flow chart describing the antibody selection process, including criteria and intended application for the scFv, Fab, and IgG formats. 
         FIGS. 12A, 12B, and 12C  depict bio-layer interferometry (BLI) results for Fab clone G5-P7A05 to HLA-PEPTIDE target B*35:01-EVDPIGHVY, Fab clones R3G8-P2C10 and G8-P1C11 to HLA-PEPTIDE target A*02:01-AIFPGAVPAA, and Fab clone R3G10-P1B07 to HLA-PEPTIDE target A*01:01-ASSLPTTMNY. 
         FIG. 13  shows a general experimental design for the positional scanning experiments. 
         FIG. 14A  shows stability results for the G5 positional variant-HLAs. 
         FIG. 14B  shows binding affinity of Fab clone G5-P7A05 to the G5 positional variant-HLAs. 
         FIG. 15A  shows stability results for the G8 positional variant-HLAs. 
         FIG. 15B  shows binding affinity of Fab clone G8-P2C10 to the G8 positional variant-HLAs. 
         FIG. 16A  shows stability results for the G10 positional variant-HLAs. 
         FIG. 16B  shows binding affinity of Fab clone G10-P1B07 to the G10 positional variant-HLAs. 
         FIGS. 17A, 17B, and 17C  show representative examples of antibody binding to either G5-, G8- or G10-presenting K562 cells, as detected by flow cytometry. 
         FIGS. 18A-18C  show histogram plots of K562 cell binding to generated target-specific antibodies. 
         FIGS. 19A-19C  show histogram plots of cell binding assays using tumor cell lines which express HLA subtypes and target genes of selected HLA-PEPTIDE targets. 
         FIGS. 20A and 20B  shows number of target-specific T cells (A) and number of target-specific unique TCR clonotypes (B) from tested donors. 
         FIG. 21A  shows an exemplary heatmap for scFv G8-P1H08, visualized across the HLA portion of HLA-PEPTIDE target G8 in its entirety using a consolidated perturbation view.  FIG. 21B  shows an example of HDX data from scFv G8-P1H08 plotted on a crystal structure 1jf1.pdb, available at http://www.rcsb.org/structure/1JF1. 
         FIG. 22A  shows heat maps across the HLA α1 helix for all ABPs tested for HLA-PEPTIDE target G8 (HLA-A*02:01_AIFPGAVPAA).  FIG. 22B  shows heat maps across the HLA α2 helix for all ABPs tested for HLA-PEPTIDE target G8 (HLA-A*02:01_AIFPGAVPAA.  FIG. 22C  shows resulting heat maps across the restricted peptide AIFPGAVPAA for all ABPs tested. 
         FIG. 23A  shows an exemplary heatmap for scFv R3G10-P2G11, visualized across the HLA portion of HLA-PEPTIDE target G10 in its entirety using a consolidated perturbation view. 
         FIG. 23B  shows an example of HDX data from scFv R3G10-P2G11 plotted on a crystal structure PDB5bs0. 
         FIG. 23C  shows an example of HDX data from scFv G10-P5A08 plotted on a crystal structure PDB5bs0. 
         FIG. 24A  shows resulting heat maps across the HLA α1 helix for all ABPs tested for HLA-PEPTIDE target G10 (HLA-A*01:01_ASSLPTTMNY).  FIG. 24B  shows resulting heat maps across the HLA α2 helix for all ABPs tested for HLA-PEPTIDE target G10 (HLA-A*01:01_ASSLPTTMNY).  FIG. 24C  shows resulting heat maps across the restricted peptide ASSLPTTMNY for all ABPs tested. 
         FIG. 25  depicts exemplary spectral data for peptide EVDPIGHVY. The figure contains the peptide fragmentation information as well as information related to the patient sample, including HLA types. 
         FIG. 26  depicts exemplary spectral data for peptide AIFPGAVPAA. The figure contains the peptide fragmentation information as well as information related to the patient sample, including HLA types. 
         FIG. 27  depicts exemplary spectral data for peptide ASSLPTTMNY. The figure contains the peptide fragmentation information as well as information related to the patient sample, including HLA types. 
         FIGS. 28A and 28B  depict size exclusion chromatography fractions (A) and SDS-PAGE analysis of the chromatography fractions under reducing conditions (B). 
         FIG. 29  depicts photomicrographs of an exemplary crystal of a complex comprising Fab clone G8-P1C11 and HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 30  depicts the overall structure of a complex formed by binding of Fab clone G8-P1C11 to HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 31  depicts a refinement electron density region of the crystal structure of Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”), the region depicted corresponding to the restricted peptide AIFPGAVPAA. 
         FIG. 32  depicts a LigPlot of the interactions between the HLA and restricted peptide. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 33  depicts a plot of interacting residues between the Fab VH and VL chains and the restricted peptide. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 34  depicts a LigPlot of the interactions between the restricted peptide and Fab chains. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 35  depicts a LigPlot of the interactions between the Fab VH chain and the HLA. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 36  depicts a LigPlot of the interactions between the Fab VL chain and the HLA. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 37  depicts the interface summary of a Pisa analysis of interactions between HLA and restricted peptide. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 38  depicts Pisa analysis of the interacting residues between the HLA and restricted peptide. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 39  depicts Pisa analysis of the interacting residues between the Fab VH chain and the restricted peptide. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 40  depicts Pisa analysis of the interacting residues between the Fab VL chain and the restricted peptide. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 41  depicts the interface summary of a Pisa analysis of interactions between the Fab VH chain and HLA. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 42  depicts Pisa analysis of the interacting residues between the Fab VH chain and HLA. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 43  depicts the interface summary of a Pisa analysis of interactions between the Fab VL chain and HLA. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 44  depicts Pisa analysis of the interacting residues between the Fab VL chain and HLA. The crystal structure corresponds to Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 45A  depicts an exemplary heatmap of the HLA portion of the G8 HLA-PEPTIDE complex when incubated with scFv clone G8-P1C11, visualized in its entirety using a consolidated perturbation view. 
         FIG. 45B  depicts an example of the HDX data from scFv G8-P1C11 plotted on a crystal structure of Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 46  depicts binding affinity of Fab clone G8-P1C11 to the G8 positional variant-HLAs. 
         FIG. 47  shows histogram plots of K562 cell binding to G8-P1C11, a target-specific antibody to HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 48  depicts an exemplary construct backbone sequence for cloning TCRs into expression systems for therapy development. 
         FIG. 49  depicts an exemplary construct sequence for cloning a TCR specific for A*0201_LLASSILCA into expression systems for therapy development. 
         FIG. 50  depicts an exemplary construct sequence for cloning a TCR specific for A*0101_EVDPIGHLY into expression systems for therapy development. 
         FIG. 51  shows spectra data for peptide EVDPIGHLY. The figure contains the peptide fragmentation information as well as information related to the patient sample, including HLA types. 
         FIG. 52  shows spectra data for peptide GVHGGILNK. The figure contains the peptide fragmentation information as well as information related to the patient sample, including HLA types. 
         FIG. 53  shows spectra data for peptide GVYDGEEHSV. 
         FIG. 54  shows spectra data for peptide NTDNNLAVY. 
         FIGS. 55-63  show spectra data for additional peptides disclosed in Table A. 
         FIG. 64  shows the design of target screen 1 for the G2 target HLA-A*01:01 NTDNNLAVY. 
         FIG. 65A  shows the target and minipool negative control design for the G2 target. 
         FIG. 65B  shows stability ELISA results for the G2 counterscreen “minipool” and G2 targets. 
         FIG. 66  shows stability ELISA results for the additional G2 “complete” pool counterscreen peptides. 
         FIG. 67  shows the design of target screen 2 for the G7 target HLA-A*02:01_LLASSILCA. 
         FIG. 68  shows stability ELISA results for the additional G7 “complete pool” counterscreen peptides. 
         FIG. 69A  shows the target and minipool negative control design for the G7 target. 
         FIG. 69B  shows stability ELISA results for the G7 counterscreen “minipool” and G7 targets. 
         FIGS. 70A and 70B  show phage panning results for the G2 and G7 targets, respectively. 
         FIGS. 71A and 71B  show biolayer interferometry (BLI) results for G2 target Fab clone G-2P1H11 and G7 target G7R4-B5-P2E9, respectively. 
         FIG. 72  shows a map of the amino acid substitutions for the positional scanning experiment described herein. 
         FIG. 73A  shows a stability heat map for the G2 positional variant-HLAs. 
         FIG. 73B  shows an affinity heat map for Fab clone G2-P1H11. 
         FIG. 74A  shows a stability heat map for the G7 positional variants. 
         FIG. 74B  shows an affinity heat map for Fab clone G7R4-B5-P2E9. 
         FIG. 75  shows cell binding results for Fab clones G2-P1H11 and G7R4-B5-P2E9 to HLA-transduced K562 cells pulsed with target or negative control peptides. 
         FIG. 76  shows cell binding results for Fab clones G2-P1H11 and G7R4-B5-P2E9 to HLA-transduced K562 cells pulsed with target or negative control peptides. 
         FIG. 77  shows an example of hydrogen-deuterium exchange (HDX) data plotted on a crystal structure PDB 5bs0. 
         FIG. 78  shows an exemplary HDX heatmap for scFv clone G2-P1G07 visualized in its entirety using a consolidated perturbation view. 
         FIG. 79  shows HDX heat maps across the HLA α1 and α2 helices for the tested G2 scFv and Fab clones. 
         FIG. 80  shows an HDX heat map across the restricted peptide NTDNNLAVY for the tested G2 scFv and Fab clones. 
         FIG. 81  depicts an experimental workflow by which TCRs which specifically bind HLA-PEPTIDE targets were isolated. 
         FIG. 82  shows a flow cytometry sorting procedure for sorting MHC-target-specific CD8+ T cells. 
         FIG. 83  shows flow cytometry results for exemplary HLA-PEPTIDE targets B*44:02_GEMSSNSTAL and A*01:01_EVDPIGHLY. 
         FIG. 84  shows flow cytometry results for the HLA-PETPIDE target A*03:01_GVHGGILNK. 
         FIG. 85A  shows total number of isolated CD8+ T cells per HLA-PEPTIDE target summed across all donors tested. 
         FIG. 85B  shows frequency of isolated CD8+ T cells per HLA-PEPTIDE target summed across all donors tested. 
         FIG. 86A  depicts the number of unique TCR clonotypes per HLA-PEPTIDE target for each tested donor. 
         FIG. 86B  depicts the total number of unique clonotypes per HLA-PEPTIDE target, summed across all donors tested. 
         FIG. 87  shows examples of Jurkat cells expressing A*0201_LLASSILCA, A*0201_GVYDGEEHSV, B*4402_GEMSSNSTAL, and A*0101_EVDPIGHLY-specific TCRs binding to their respective HLA-PEPTIDE targets but not to the control peptide tetramer. 
         FIG. 88  shows the gating strategy and flow data demonstrating that human CD8+ cells transduced with TCRs identified herein bind to their specific HLA-PEPTIDE target. 
         FIG. 89  shows an exemplary lentiviral vector useful for transducing recipient cells with a TCR disclosed herein. 
         FIG. 90  shows BLI results for G2 target Fab clone G2-P2C06. 
         FIG. 91A  depicts stability results from a second experiment for the G2 positional variant-HLAs. 
         FIG. 91B  depicts binding affinity of Fab clone G2-P2C06 to the G2 positional variant-HLAs. 
         FIG. 92  shows HDX heat maps from a second round of HDX experiments across the HLA α1 helix, the HLA α2 helix, and the restricted peptide ASSLPTTMNY for various G10 ABPs tested. 
         FIG. 93  shows HDX heat maps from a second round of HDX experiments across the HLA α1 helix, the HLA α2 helix, and the restricted peptide NTDNNLAVY for G2 ABPs tested. 
         FIG. 94  shows an example of HDX data from scFv G2-P2C11 plotted on a crystal structure PDB 5bs0. 
         FIG. 95  shows high resolution HDX data plotted on a crystal structure PDB 5bs0. Data for G2 bound to four different scFvs were obtained by fragmenting peptides by Electron Transfer Dissociation (ETD) as described in the Experimental Procedures. The peptide fragments with high-resolution data (at approximately single amino-acid resolution) and residues 157-160 are encircled. 
         FIG. 96  shows color heat maps from HDX experiments across the HLA α1 helix, the HLA α2 helix, and restricted peptide EVDPIGHVY for all ABPs tested for HLA-PEPTIDE target G5 (HLA-B*35:01_EVDPIGHVY). 
         FIG. 97  shows a numerical representation of the color heat map of  FIG. 96 . 
         FIG. 98  shows an example of data from scFv clone G5-P1C12 plotted on crystal structure of HLA-B*35:01 (5xos.pdb; https://www.rcsb.org/structure/5XOS). 
         FIG. 99  shows color heat maps from a second round of HDX experiments across the HLA α1 helix, the HLA α2 helix, and restricted peptide AIFPGAVPAA for all ABPs tested for HLA-PEPTIDE target G8 (HLA-A*02:01_AIFPGAVPAA). 
         FIG. 100  shows a numerical representation of the color heat maps of  FIG. 99 . 
         FIG. 101  shows an example of high-resolution HDX data from scFv G8-P1H08 plotted on a crystal structure of Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”). 
         FIG. 102  shows results from a flow cytometry experiment wherein HLA-B*35:01-transduced K562 cells were pulsed with 50 μM of target peptide EVDPIGHVY (“EVD”) or negative control peptide IPSINVHHY (“IPS”), and pHLA-specific antibodies were detected by flow cytometry. 
         FIG. 103  shows results from a flow cytometry experiment wherein HLA-A*02:01-transduced K562 cells were pulsed with 50 μM of target peptide AIFPGAVPAA (“AIF”) or negative control peptide FLLTRILTI (“FLL”), and pHLA-specific antibodies were detected by flow cytometry. 
         FIG. 104  shows results from a flow cytometry experiment wherein HLA-A*01:01-transduced K562 cells were pulsed with 50 μM of target peptide ASSLPTTMNY (“ASSL”) or negative control peptide ATDALMTGY (“ATDA”), and pHLA-specific antibodies were detected by flow cytometry. 
         FIG. 105  shows BLI results for G8 target Fab clones G8-P4F05, G8-P1B03, and G8-P5G08 to HLA-PEPTIDE target A*02:01-AIFPGAVPAA; as well as BLI results for G5 target Fab clone G5-P1C12 to HLA-PEPTIDE target B*35:01-EVDPIGHVY. 
     
    
    
     DETAILED DESCRIPTION 
     Unless otherwise defined, all terms of art, notations and other scientific terminology used herein are intended to have the meanings commonly understood by those of skill in the art. In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference, and the inclusion of such definitions herein should not necessarily be construed to represent a difference over what is generally understood in the art. The techniques and procedures described or referenced herein are generally well understood and commonly employed using conventional methodologies by those skilled in the art, such as, for example, the widely utilized molecular cloning methodologies described in Sambrook et al., Molecular Cloning: A Laboratory Manual 4th ed. (2012) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. As appropriate, procedures involving the use of commercially available kits and reagents are generally carried out in accordance with manufacturer-defined protocols and conditions unless otherwise noted. 
     As used herein, the singular forms “a,” “an,” and “the” include the plural referents unless the context clearly indicates otherwise. The terms “include,” “such as,” and the like are intended to convey inclusion without limitation, unless otherwise specifically indicated. 
     As used herein, the term “comprising” also specifically includes embodiments “consisting of” and “consisting essentially of” the recited elements, unless specifically indicated otherwise. For example, a multispecific ABP “comprising a diabody” includes a multispecific ABP “consisting of a diabody” and a multispecific ABP “consisting essentially of a diabody.” 
     The term “about” indicates and encompasses an indicated value and a range above and below that value. In certain embodiments, the term “about” indicates the designated value+10%, +5%, or +1%. In certain embodiments, where applicable, the term “about” indicates the designated value(s)±one standard deviation of that value(s). 
     The term “immunoglobulin” refers to a class of structurally related proteins generally comprising two pairs of polypeptide chains: one pair of light (L) chains and one pair of heavy (H) chains. In an “intact immunoglobulin,” all four of these chains are interconnected by disulfide bonds. The structure of immunoglobulins has been well characterized. See, e.g., Paul,  Fundamental Immunology  7th ed., Ch. 5 (2013) Lippincott Williams &amp; Wilkins, Philadelphia, Pa. Briefly, each heavy chain typically comprises a heavy chain variable region (VH) and a heavy chain constant region (CH). The heavy chain constant region typically comprises three domains, abbreviated C H1 , C H2 , and C H3 . Each light chain typically comprises a light chain variable region (V L ) and a light chain constant region. The light chain constant region typically comprises one domain, abbreviated C L . 
     The term “antigen binding protein” or “ABP” is used herein in its broadest sense and includes certain types of molecules comprising one or more antigen-binding domains that specifically bind to an antigen or epitope. 
     In some embodiments, the ABP comprises an antibody. In some embodiments, the ABP consists of an antibody. In some embodiments, the ABP consists essentially of an antibody. An ABP specifically includes intact antibodies (e.g., intact immunoglobulins), antibody fragments, ABP fragments, and multi-specific antibodies. In some embodiments, the ABP comprises an alternative scaffold. In some embodiments, the ABP consists of an alternative scaffold. In some embodiments, the ABP consists essentially of an alternative scaffold. In some embodiments, the ABP comprises an antibody fragment. In some embodiments, the ABP consists of an antibody fragment. In some embodiments, the ABP consists essentially of an antibody fragment. In some embodiments, the ABP comprises a TCR or antigen binding portion thereof. In some embodiments, the ABP consists of a TCR or antigen binding portion thereof. In some embodiments, the ABP consists essentially of a TCR or antigen binding portion thereof. In some embodiments, a CAR comprises an ABP. An “HLA-PEPTIDE ABP,” “anti-HLA-PEPTIDE ABP,” or “HLA-PEPTIDE-specific ABP” is an ABP, as provided herein, which specifically binds to the antigen HLA-PEPTIDE. An ABP includes proteins comprising one or more antigen-binding domains that specifically bind to an antigen or epitope via a variable region, such as a variable region derived from a B cell (e.g., antibody) or T cell (e.g., TCR). 
     The term “antibody” herein is used in the broadest sense and includes polyclonal and monoclonal antibodies, including intact antibodies and functional (antigen-binding) antibody fragments, including fragment antigen binding (Fab) fragments, F(ab′)2 fragments, Fab′ fragments, Fv fragments, recombinant IgG (rIgG) fragments, variable heavy chain (VH) regions capable of specifically binding the antigen, single chain antibody fragments, including single chain variable fragments (scFv), and single domain antibodies (e.g., sdAb, sdFv, nanobody) fragments. The term encompasses genetically engineered and/or otherwise modified forms of immunoglobulins, such as intrabodies, peptibodies, chimeric antibodies, fully human antibodies, humanized antibodies, and heteroconjugate antibodies, multispecific, e.g., bispecific, antibodies, diabodies, triabodies, and tetrabodies, tandem di-scFv, tandem tri-scFv. Unless otherwise stated, the term “antibody” should be understood to encompass functional antibody fragments thereof. The term also encompasses intact or full-length antibodies, including antibodies of any class or sub-class, including IgG and sub-classes thereof, IgM, IgE, IgA, and IgD. 
     As used herein, “variable region” refers to a variable nucleotide sequence that arises from a recombination event, for example, it can include a V, J, and/or D region of an immunoglobulin or T cell receptor (TCR) sequence from a B cell or T cell, such as an activated T cell or an activated B cell. 
     The term “antigen-binding domain” means the portion of an ABP that is capable of specifically binding to an antigen or epitope. One example of an antigen-binding domain is an antigen-binding domain formed by an antibody V H -V L  dimer of an ABP. Another example of an antigen-binding domain is an antigen-binding domain formed by diversification of certain loops from the tenth fibronectin type III domain of an Adnectin. An antigen-binding domain can include antibody CDRs 1, 2, and 3 from a heavy chain in that order; and antibody CDRs 1, 2, and 3 from a light chain in that order. An antigen-binding domain can include TCR CDRs, e.g., αCDR1, αCDR2, αCDR3, βCDR1, βCDR2, and βCDR3. TCR CDRs are described herein. 
     The antibody V H  and V L  regions may be further subdivided into regions of hypervariability (“hypervariable regions (HVRs);” also called “complementarity determining regions” (CDRs)) interspersed with regions that are more conserved. The more conserved regions are called framework regions (FRs). Each V H  and V L  generally comprises three antibody CDRs and four FRs, arranged in the following order (from N-terminus to C-terminus): FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4. The antibody CDRs are involved in antigen binding, and influence antigen specificity and binding affinity of the ABP. See Kabat et al.,  Sequences of Proteins of Immunological Interest  5th ed. (1991) Public Health Service, National Institutes of Health, Bethesda, Md., incorporated by reference in its entirety. 
     The light chain from any vertebrate species can be assigned to one of two types, called kappa (κ) and lambda (λ), based on the sequence of its constant domain. 
     The heavy chain from any vertebrate species can be assigned to one of five different classes (or isotypes): IgA, IgD, IgE, IgG, and IgM. These classes are also designated α, δ, ε, γ, and μ, respectively. The IgG and IgA classes are further divided into subclasses on the basis of differences in sequence and function. Humans express the following subclasses: IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2. 
     The amino acid sequence boundaries of an antibody CDR can be determined by one of skill in the art using any of a number of known numbering schemes, including those described by Kabat et al., supra (“Kabat” numbering scheme); Al-Lazikani et al., 1997 , J Mol. Biol.,  273:927-948 (“Chothia” numbering scheme); MacCallum et al., 1996 , J. Mol. Biol.  262:732-745 (“Contact” numbering scheme); Lefranc et al.,  Dev. Comp. Immunol.,  2003, 27:55-77 (“IMGT” numbering scheme); and Honegge and Plückthun,  J. Mol. Biol.,  2001, 309:657-70 (“AHo” numbering scheme); each of which is incorporated by reference in its entirety. 
     Table 20 provides the positions of antibody CDR-L1, CDR-L2, CDR-L3, CDR-H1, CDR-H2, and CDR-H3 as identified by the Kabat and Chothia schemes. For CDR-H1, residue numbering is provided using both the Kabat and Chothia numbering schemes. 
     Antibody CDRs may be assigned, for example, using ABP numbering software, such as Abnum, available at www.bioinf.org.uk/abs/abnum/, and described in Abhinandan and Martin, Immunology, 2008, 45:3832-3839, incorporated by reference in its entirety. 
     
       
         
           
               
             
               
                 TABLE 20 
               
             
            
               
                   
               
               
                 Residues in CDRs according to Kabat and Chothia numbering schemes 
               
            
           
           
               
               
               
            
               
                 CDR 
                 Kabat 
                 Chothia 
               
               
                   
               
               
                 L1 
                 L24-L34 
                 L24-L34 
               
               
                 L2 
                 L50-L56 
                 L50-L56 
               
               
                 L3 
                 L89-L97 
                 L89-L97 
               
               
                 H1 (Kabat Numbering) 
                 H31-H35B 
                 H26-H32 or H34* 
               
               
                 H1 (Chothia Numbering) 
                 H31-H35 
                 H26-H32 
               
               
                 H2 
                 H50-H65 
                 H52-H56 
               
               
                 H3 
                 H95-H102 
                 H95-H102 
               
               
                   
               
               
                 *The C-terminus of CDR-H1, when numbered using the Kabat numbering convention, varies between H32 and H34, depending on the length of the CDR. 
               
            
           
         
       
     
     The “EU numbering scheme” is generally used when referring to a residue in an ABP heavy chain constant region (e.g., as reported in Kabat et al., supra). Unless stated otherwise, the EU numbering scheme is used to refer to residues in ABP heavy chain constant regions described herein. 
     The terms “full length antibody,” “intact antibody,” and “whole antibody” are used herein interchangeably to refer to an antibody having a structure substantially similar to a naturally occurring antibody structure and having heavy chains that comprise an Fc region. For example, when used to refer to an IgG molecule, a “full length antibody” is an antibody that comprises two heavy chains and two light chains. 
     The amino acid sequence boundaries of a TCR CDR can be determined by one of skill in the art using any of a number of known numbering schemes, including but not limited to the IMGT unique numbering, as described by LeFranc, M.-P, Immunol Today. 1997 November; 18(11):509; Lefranc, M.-P., “IMGT Locus on Focus: A new section of Experimental and Clinical Immunogenetics”, Exp. Clin. Immunogenet., 15, 1-7 (1998); Lefranc and Lefranc, The T Cell Receptor FactsBook; and M.-P. Lefranc/Developmental and Comparative Immunology 27 (2003) 55-77, all of which are incorporated by reference. 
     An “ABP fragment” comprises a portion of an intact ABP, such as the antigen-binding or variable region of an intact ABP. ABP fragments include, for example, Fv fragments, Fab fragments, F(ab′)2 fragments, Fab′ fragments, scFv (sFv) fragments, and scFv-Fc fragments. ABP fragments include antibody fragments. Antibody fragments can include Fv fragments, Fab fragments, F(ab′)2 fragments, Fab′ fragments, scFv (sFv) fragments, scFv-Fc fragments, and TCR fragments. 
     “Fv” fragments comprise a non-covalently-linked dimer of one heavy chain variable domain and one light chain variable domain. 
     “Fab” fragments comprise, in addition to the heavy and light chain variable domains, the constant domain of the light chain and the first constant domain (CHI) of the heavy chain. Fab fragments may be generated, for example, by recombinant methods or by papain digestion of a full-length ABP. 
     “F(ab′) 2 ” fragments contain two Fab′ fragments joined, near the hinge region, by disulfide bonds. F(ab′) 2  fragments may be generated, for example, by recombinant methods or by pepsin digestion of an intact ABP. The F(ab′) fragments can be dissociated, for example, by treatment with β-mercaptoethanol. 
     “Single-chain Fv” or “sFv” or “scFv” fragments comprise a VH domain and a VL domain in a single polypeptide chain. The VH and VL are generally linked by a peptide linker. See Plückthun A. (1994). Any suitable linker may be used. In some embodiments, the linker is a (GGGGS) n . In some embodiments, n=1, 2, 3, 4, 5, or 6. See ABPs from  Escherichia coli . In Rosenberg M. &amp; Moore G. P. (Eds.),  The Pharmacology of Monoclonal ABPs  vol. 113 (pp. 269-315). Springer-Verlag, New York, incorporated by reference in its entirety. 
     “scFv-Fc” fragments comprise an scFv attached to an Fc domain. For example, an Fc domain may be attached to the C-terminal of the scFv. The Fc domain may follow the V H  or V L , depending on the orientation of the variable domains in the scFv (i.e., V H -V L  or V L -V H ). Any suitable Fc domain known in the art or described herein may be used. In some cases, the Fc domain comprises an IgG4 Fc domain. 
     The term “single domain antibody” refers to a molecule in which one variable domain of an ABP specifically binds to an antigen without the presence of the other variable domain. Single domain ABPs, and fragments thereof, are described in Arabi Ghahroudi et al.,  FEBS Letters,  1998, 414:521-526 and Muyldermans et al.,  Trends in Biochem. Sci.,  2001, 26:230-245, each of which is incorporated by reference in its entirety. Single domain ABPs are also known as sdAbs or nanobodies. 
     The term “Fe region” or “Fc” means the C-terminal region of an immunoglobulin heavy chain that, in naturally occurring antibodies, interacts with Fc receptors and certain proteins of the complement system. The structures of the Fc regions of various immunoglobulins, and the glycosylation sites contained therein, are known in the art. See Schroeder and Cavacini,  J. Allergy Clin. Immunol.,  2010, 125:S41-52, incorporated by reference in its entirety. The Fc region may be a naturally occurring Fc region, or an Fc region modified as described in the art or elsewhere in this disclosure. 
     The term “alternative scaffold” refers to a molecule in which one or more regions may be diversified to produce one or more antigen-binding domains that specifically bind to an antigen or epitope. In some embodiments, the antigen-binding domain binds the antigen or epitope with specificity and affinity similar to that of an ABP. Exemplary alternative scaffolds include those derived from fibronectin (e.g., Adnectins™), the β-sandwich (e.g., iMab), lipocalin (e.g., Anticalins®), EETI-II/AGRP, BPTI/LACI-D1/ITI-D2 (e.g., Kunitz domains), thioredoxin peptide aptamers, protein A (e.g., Affibody®), ankyrin repeats (e.g., DARPins), gamma-B-crystallin/ubiquitin (e.g., Affilins), CTLD3 (e.g., Tetranectins), Fynomers, and (LDLR-A module) (e.g., Avimers). Additional information on alternative scaffolds is provided in Binz et al.,  Nat. Biotechnol.,  2005 23:1257-1268; Skerra,  Current Opin. in Biotech.,  2007 18:295-304; and Silacci et al.,  J. Biol. Chem.,  2014, 289:14392-14398; each of which is incorporated by reference in its entirety. An alternative scaffold is one type of ABP. 
     A “multispecific ABP” is an ABP that comprises two or more different antigen-binding domains that collectively specifically bind two or more different epitopes. The two or more different epitopes may be epitopes on the same antigen (e.g., a single HLA-PEPTIDE molecule expressed by a cell) or on different antigens (e.g., different HLA-PEPTIDE molecules expressed by the same cell, or a HLA-PEPTIDE molecule and a non-HLA-PEPTIDE molecule). In some aspects, a multi-specific ABP binds two different epitopes (i.e., a “bispecific ABP”). In some aspects, a multi-specific ABP binds three different epitopes (i.e., a “trispecific ABP”). 
     A “monospecific ABP” is an ABP that comprises one or more binding sites that specifically bind to a single epitope. An example of a monospecific ABP is a naturally occurring IgG molecule which, while divalent (i.e., having two antigen-binding domains), recognizes the same epitope at each of the two antigen-binding domains. The binding specificity may be present in any suitable valency. 
     The term “monoclonal antibody” refers to an antibody from a population of substantially homogeneous antibodies. A population of substantially homogeneous antibodies comprises antibodies that are substantially similar and that bind the same epitope(s), except for variants that may normally arise during production of the monoclonal antibody. Such variants are generally present in only minor amounts. A monoclonal antibody is typically obtained by a process that includes the selection of a single antibody from a plurality of antibodies. For example, the selection process can be the selection of a unique clone from a plurality of clones, such as a pool of hybridoma clones, phage clones, yeast clones, bacterial clones, or other recombinant DNA clones. The selected antibody can be further altered, for example, to improve affinity for the target (“affinity maturation”), to humanize the antibody, to improve its production in cell culture, and/or to reduce its immunogenicity in a subject. 
     The term “chimeric antibody” refers to an antibody in which a portion of the heavy and/or light chain is derived from a particular source or species, while the remainder of the heavy and/or light chain is derived from a different source or species. 
     “Humanized” forms of non-human antibodies are chimeric antibodies that contain minimal sequence derived from the non-human antibody. A humanized antibody is generally a human antibody (recipient antibody) in which residues from one or more CDRs are replaced by residues from one or more CDRs of a non-human antibody (donor antibody). The donor antibody can be any suitable non-human antibody, such as a mouse, rat, rabbit, chicken, or non-human primate antibody having a desired specificity, affinity, or biological effect. In some instances, selected framework region residues of the recipient antibody are replaced by the corresponding framework region residues from the donor antibody. Humanized antibodies may also comprise residues that are not found in either the recipient antibody or the donor antibody. Such modifications may be made to further refine antibody function. For further details, see Jones et al.,  Nature,  1986, 321:522-525; Riechmann et al.,  Nature,  1988, 332:323-329; and Presta,  Curr. Op. Struct. Biol.,  1992, 2:593-596, each of which is incorporated by reference in its entirety. 
     A “human antibody” is one which possesses an amino acid sequence corresponding to that of an antibody produced by a human or a human cell, or derived from a non-human source that utilizes a human antibody repertoire or human antibody-encoding sequences (e.g., obtained from human sources or designed de novo). Human antibodies specifically exclude humanized antibodies. 
     “Affinity” refers to the strength of the sum total of non-covalent interactions between a single binding site of a molecule (e.g., an ABP) and its binding partner (e.g., an antigen or epitope). Unless indicated otherwise, as used herein, “affinity” refers to intrinsic binding affinity, which reflects a 1:1 interaction between members of a binding pair (e.g., ABP and antigen or epitope). The affinity of a molecule X for its partner Y can be represented by the dissociation equilibrium constant (K D ). The kinetic components that contribute to the dissociation equilibrium constant are described in more detail below. Affinity can be measured by common methods known in the art, including those described herein, such as surface plasmon resonance (SPR) technology (e.g., BIACORE®) or biolayer interferometry (e.g., FORTEBIO®). 
     With regard to the binding of an ABP to a target molecule, the terms “bind,” “specific binding,” “specifically binds to,” “specific for,” “selectively binds,” and “selective for” a particular antigen (e.g., a polypeptide target) or an epitope on a particular antigen mean binding that is measurably different from a non-specific or non-selective interaction (e.g., with a non-target molecule). Specific binding can be measured, for example, by measuring binding to a target molecule and comparing it to binding to a non-target molecule. Specific binding can also be determined by competition with a control molecule that mimics the epitope recognized on the target molecule. In that case, specific binding is indicated if the binding of the ABP to the target molecule is competitively inhibited by the control molecule. In some aspects, the affinity of a HLA-PEPTIDE ABP for a non-target molecule is less than about 50% of the affinity for HLA-PEPTIDE. In some aspects, the affinity of a HLA-PEPTIDE ABP for a non-target molecule is less than about 40% of the affinity for HLA-PEPTIDE. In some aspects, the affinity of a HLA-PEPTIDE ABP for a non-target molecule is less than about 30% of the affinity for HLA-PEPTIDE. In some aspects, the affinity of a HLA-PEPTIDE ABP for a non-target molecule is less than about 20% of the affinity for HLA-PEPTIDE. In some aspects, the affinity of a HLA-PEPTIDE ABP for a non-target molecule is less than about 10% of the affinity for HLA-PEPTIDE. In some aspects, the affinity of a HLA-PEPTIDE ABP for a non-target molecule is less than about 1% of the affinity for HLA-PEPTIDE. In some aspects, the affinity of a HLA-PEPTIDE ABP for a non-target molecule is less than about 0.1% of the affinity for HLA-PEPTIDE. 
     The term “k d ” (sec −1 ), as used herein, refers to the dissociation rate constant of a particular ABP-antigen interaction. This value is also referred to as the k off  value. 
     The term “k d ” (M −1 ×sec −1 ), as used herein, refers to the association rate constant of a particular ABP-antigen interaction. This value is also referred to as the k on  value. 
     The term “K D ” (M), as used herein, refers to the dissociation equilibrium constant of a particular ABP-antigen interaction. K D =k d /k a . In some embodiments, the affinity of an ABP is described in terms of the K D  for an interaction between such ABP and its antigen. For clarity, as known in the art, a smaller K D  value indicates a higher affinity interaction, while a larger K D  value indicates a lower affinity interaction. 
     The term “K A ” (M −1 ), as used herein, refers to the association equilibrium constant of a particular ABP-antigen interaction. K A =k a /k d . 
     An “immunoconjugate” is an ABP conjugated to one or more heterologous molecule(s), such as a therapeutic (cytokine, for example) or diagnostic agent. 
     “Fc effector functions” refer to those biological activities mediated by the Fc region of an ABP having an Fc region, which activities may vary depending on isotype. Examples of ABP effector functions include C1q binding to activate complement dependent cytotoxicity (CDC), Fc receptor binding to activate ABP-dependent cellular cytotoxicity (ADCC), and ABP dependent cellular phagocytosis (ADCP). 
     When used herein in the context of two or more ABPs, the term “competes with” or “cross-competes with” indicates that the two or more ABPs compete for binding to an antigen (e.g., HLA-PEPTIDE). In one exemplary assay, HLA-PEPTIDE is coated on a surface and contacted with a first HLA-PEPTIDE ABP, after which a second HLA-PEPTIDE ABP is added. In another exemplary assay, a first HLA-PEPTIDE ABP is coated on a surface and contacted with HLA-PEPTIDE, and then a second HLA-PEPTIDE ABP is added. If the presence of the first HLA-PEPTIDE ABP reduces binding of the second HLA-PEPTIDE ABP, in either assay, then the ABPs compete with each other. The term “competes with” also includes combinations of ABPs where one ABP reduces binding of another ABP, but where no competition is observed when the ABPs are added in the reverse order. However, in some embodiments, the first and second ABPs inhibit binding of each other, regardless of the order in which they are added. In some embodiments, one ABP reduces binding of another ABP to its antigen by at least 25%, at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, or at least 95%. A skilled artisan can select the concentrations of the ABPs used in the competition assays based on the affinities of the ABPs for HLA-PEPTIDE and the valency of the ABPs. The assays described in this definition are illustrative, and a skilled artisan can utilize any suitable assay to determine if ABPs compete with each other. Suitable assays are described, for example, in Cox et al., “Immunoassay Methods,” in  Assay Guidance Manual [ Internet ], Updated Dec. 24, 2014 (www.ncbi.nlm.nih.gov/books/NBK92434/; accessed Sep. 29, 2015); Silman et al.,  Cytometry,  2001, 44:30-37; and Finco et al.,  J. Pharm. Biomed. Anal.,  2011, 54:351-358; each of which is incorporated by reference in its entirety. 
     The term “epitope” means a portion of an antigen that specifically binds to an ABP. Epitopes frequently consist of surface-accessible amino acid residues and/or sugar side chains and may have specific three dimensional structural characteristics, as well as specific charge characteristics. Conformational and non-conformational epitopes are distinguished in that the binding to the former but not the latter may be lost in the presence of denaturing solvents. An epitope may comprise amino acid residues that are directly involved in the binding, and other amino acid residues, which are not directly involved in the binding. The epitope to which an ABP binds can be determined using known techniques for epitope determination such as, for example, testing for ABP binding to HLA-PEPTIDE variants with different point-mutations, or to chimeric HLA-PEPTIDE variants. 
     Percent “identity” between a polypeptide sequence and a reference sequence, is defined as the percentage of amino acid residues in the polypeptide sequence that are identical to the amino acid residues in the reference sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved in various ways that are within the skill in the art, for instance, using publicly available computer software such as BLAST, BLAST-2, ALIGN, MEGALIGN (DNASTAR), CLUSTALW, CLUSTAL OMEGA, or MUSCLE software. Those skilled in the art can determine appropriate parameters for aligning sequences, including any algorithms needed to achieve maximal alignment over the full length of the sequences being compared. 
     A “conservative substitution” or a “conservative amino acid substitution,” refers to the substitution an amino acid with a chemically or functionally similar amino acid. Conservative substitution tables providing similar amino acids are well known in the art. By way of example, the groups of amino acids provided in Tables 21-23 are, in some embodiments, considered conservative substitutions for one another. 
     
       
         
           
               
             
               
                 TABLE 21 
               
               
                   
               
               
                 Selected groups of amino acids that are considered conservative 
               
               
                 substitutions for one another, in certain embodiments. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Acidic Residues 
                 D and E 
               
               
                 Basic Residues 
                 K, R, and H 
               
               
                 Hydrophilic Uncharged Residues 
                 S, T, N, and Q 
               
               
                 Aliphatic Uncharged Residues 
                 G, A, V, L, and I 
               
               
                 Non-polar Uncharged Residues 
                 C, M, and P 
               
               
                 Aromatic Residues 
                 F, Y, and W 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 22 
               
               
                   
               
               
                 Additional selected groups of amino acids that are considered conservative 
               
               
                 substitutions for one another, in certain embodiments. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Group 1 
                 A, S, and T 
               
               
                 Group 2 
                 D and E 
               
               
                 Group 3 
                 N and Q 
               
               
                 Group 4 
                 R and K 
               
               
                 Group 5 
                 I, L, and M 
               
               
                 Group 6 
                 F, Y, and W 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 23 
               
               
                   
               
               
                 Further selected groups of amino acids that are considered conservative 
               
               
                 substitutions for one another, in certain embodiments. 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
            
               
                 Group A 
                 A and G 
               
               
                 Group B 
                 D and E 
               
               
                 Group C 
                 N and Q 
               
               
                 Group D 
                 R, K, and H 
               
               
                 Group E 
                 I, L, M, V 
               
               
                 Group F 
                 F, Y, and W 
               
               
                 Group G 
                 S and T 
               
               
                 Group H 
                 C and M 
               
               
                   
               
            
           
         
       
     
     Additional conservative substitutions may be found, for example, in Creighton,  Proteins: Structures and Molecular Properties  2nd ed. (1993) W. H. Freeman &amp; Co., New York, N.Y. An ABP generated by making one or more conservative substitutions of amino acid residues in a parent ABP is referred to as a “conservatively modified variant.” 
     The term “amino acid” refers to the twenty common naturally occurring amino acids. Naturally occurring amino acids include alanine (Ala; A), arginine (Arg; R), asparagine (Asn; N), aspartic acid (Asp; D), cysteine (Cys; C); glutamic acid (Glu; E), glutamine (Gln; Q), Glycine (Gly; G); histidine (His; H), isoleucine (Ile; I), leucine (Leu; L), lysine (Lys; K), methionine (Met; M), phenylalanine (Phe; F), proline (Pro; P), serine (Ser; S), threonine (Thr; T), tryptophan (Trp; W), tyrosine (Tyr; Y), and valine (Val; V). 
     The term “vector,” as used herein, refers to a nucleic acid molecule capable of propagating another nucleic acid to which it is linked. The term includes the vector as a self-replicating nucleic acid structure as well as the vector incorporated into the genome of a host cell into which it has been introduced. Certain vectors are capable of directing the expression of nucleic acids to which they are operatively linked. Such vectors are referred to herein as “expression vectors.” 
     The terms “host cell,” “host cell line,” and “host cell culture” are used interchangeably and refer to cells into which an exogenous nucleic acid has been introduced, and the progeny of such cells. Host cells include “transformants” (or “transformed cells”) and “transfectants” (or “transfected cells”), which each include the primary transformed or transfected cell and progeny derived therefrom. Such progeny may not be completely identical in nucleic acid content to a parent cell, and may contain mutations. 
     The term “treating” (and variations thereof such as “treat” or “treatment”) refers to clinical intervention in an attempt to alter the natural course of a disease or condition in a subject in need thereof. Treatment can be performed both for prophylaxis and during the course of clinical pathology. Desirable effects of treatment include preventing occurrence or recurrence of disease, alleviation of symptoms, diminishment of any direct or indirect pathological consequences of the disease, preventing metastasis, decreasing the rate of disease progression, amelioration or palliation of the disease state, and remission or improved prognosis. 
     As used herein, the term “therapeutically effective amount” or “effective amount” refers to an amount of an ABP or pharmaceutical composition provided herein that, when administered to a subject, is effective to treat a disease or disorder. 
     As used herein, the term “subject” means a mammalian subject. Exemplary subjects include humans, monkeys, dogs, cats, mice, rats, cows, horses, camels, goats, rabbits, and sheep. In certain embodiments, the subject is a human. In some embodiments the subject has a disease or condition that can be treated with an ABP provided herein. In some aspects, the disease or condition is a cancer. In some aspects, the disease or condition is a viral infection. 
     The term “package insert” is used to refer to instructions customarily included in commercial packages of therapeutic or diagnostic products (e.g., kits) that contain information about the indications, usage, dosage, administration, combination therapy, contraindications and/or warnings concerning the use of such therapeutic or diagnostic products. 
     The term “tumor” refers to all neoplastic cell growth and proliferation, whether malignant or benign, and all pre-cancerous and cancerous cells and tissues. The terms “cancer,” “cancerous,” “cell proliferative disorder,” “proliferative disorder” and “tumor” are not mutually exclusive as referred to herein. The terms “cell proliferative disorder” and “proliferative disorder” refer to disorders that are associated with some degree of abnormal cell proliferation. In some embodiments, the cell proliferative disorder is a cancer. In some aspects, the tumor is a solid tumor. In some aspects, the tumor is a hematologic malignancy. 
     The term “pharmaceutical composition” refers to a preparation which is in such form as to permit the biological activity of an active ingredient contained therein to be effective in treating a subject, and which contains no additional components which are unacceptably toxic to the subject in the amounts provided in the pharmaceutical composition. 
     The terms “modulate” and “modulation” refer to reducing or inhibiting or, alternatively, activating or increasing, a recited variable. 
     The terms “increase” and “activate” refer to an increase of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 100%, 2-fold, 3-fold, 4-fold, 5-fold, 10-fold, 20-fold, 50-fold, 100-fold, or greater in a recited variable. 
     The terms “reduce” and “inhibit” refer to a decrease of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 2-fold, 3-fold, 4-fold, 5-fold, 10-fold, 20-fold, 50-fold, 100-fold, or greater in a recited variable. 
     The term “agonize” refers to the activation of receptor signaling to induce a biological response associated with activation of the receptor. An “agonist” is an entity that binds to and agonizes a receptor. 
     The term “antagonize” refers to the inhibition of receptor signaling to inhibit a biological response associated with activation of the receptor. An “antagonist” is an entity that binds to and antagonizes a receptor. 
     The terms “nucleic acids” and “polynucleotides” may be used interchangeably herein to refer to polymeric form of nucleotides of any length, either deoxyribonucleotides or ribonucleotides, or analogs thereof. Polynucleotides can include, but are not limited to coding or non-coding regions of a gene or gene fragment, loci (locus) defined from linkage analysis, exons, introns, messenger RNA (mRNA), cDNA, recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA, isolated RNA, nucleic acid probes, and primers. A polynucleotide may comprise modified nucleotides, such as methylated nucleotides and nucleotide analogs. Exemplary modified nucleotides include, e.g., 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 5-(carboxyhydroxymethyl) uracil, 5-carboxymethylaminomethyl-2-thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-substituted adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5′-methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthioN6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, 3-(3-amino-3-N-2-carboxypropyl) uracil, and 2,6-diaminopurine. 
     Isolated HLA-Peptide Targets 
     The major histocompatibility complex (MHC) is a complex of antigens encoded by a group of linked loci, which are collectively termed H-2 in the mouse and HLA in humans. The two principal classes of the MHC antigens, class I and class II, each comprise a set of cell surface glycoproteins which play a role in determining tissue type and transplant compatibility. In transplantation reactions, cytotoxic T-cells (CTLs) respond mainly against class I glycoproteins, while helper T-cells respond mainly against class II glycoproteins. 
     Human major histocompatibility complex (MHC) class I molecules, referred to interchangeably herein as HLA Class I molecules, are expressed on the surface of nearly all cells. These molecules function in presenting peptides which are mainly derived from endogenously synthesized proteins to, e.g., CD8+ T cells via an interaction with the alpha-beta T-cell receptor. The class I MHC molecule comprises a heterodimer composed of a 46-kDa a chain which is non-covalently associated with the 12-kDa light chain beta-2 microglobulin. The α chain generally comprises α1 and α2 domains which form a groove for presenting an HLA-restricted peptide, and an α3 plasma membrane-spanning domain which interacts with the CD8 co-receptor of T-cells.  FIG. 1  (prior art) depicts the general structure of a Class I HLA molecule. Some TCRs can bind MHC class I independently of CD8 coreceptor (see, e.g., Kerry S E, Buslepp J, Cramer L A, et al. Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Affinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement. Journal of immunology (Baltimore, Md.: 1950). 2003; 171(9):4493-4503.) 
     Class I MHC-restricted peptides (also referred to interchangeably herein as HLA-restricted antigens, HLA-restricted peptides, MHC-restricted antigens, restricted peptides, or peptides) generally bind to the heavy chain alphal-alpha2 groove via about two or three anchor residues that interact with corresponding binding pockets in the MHC molecule. The beta-2 microglobulin chain plays an important role in MHC class I intracellular transport, peptide binding, and conformational stability. For most class I molecules, the formation of a heterotrimeric complex of the MHC class I heavy chain, peptide (self, non-self, and/or antigenic) and beta-2 microglobulin leads to protein maturation and export to the cell-surface. 
     Binding of a given HLA subtype to an HLA-restricted peptide forms a complex with a unique and novel surface that can be specifically recognized by an ABP such as, e.g., a TCR on a T cell or an antibody or antigen-binding fragment thereof. HLA complexed with an HLA-restricted peptide is referred to herein as an HLA-PEPTIDE, a pHLA, or HLA-PEPTIDE target. In some cases the restricted peptide is located in the α1/α2 groove of the HLA molecule. In some cases the restricted peptide is bound to the α1/α2 groove of the HLA molecule via about two or three anchor residues that interact with corresponding binding pockets in the HLA molecule. 
     Accordingly, provided herein are antigens comprising HLA-PEPTIDE targets. The HLA-PEPTIDE targets may comprise a specific HLA-restricted peptide having a defined amino acid sequence complexed with a specific HLA subtype. 
     HLA-PEPTIDE targets identified herein may be useful for cancer immunotherapy. In some embodiments, the HLA-PEPTIDE targets identified herein are presented on the surface of a tumor cell. The HLA-PEPTIDE targets identified herein may be expressed by tumor cells in a human subject. The HLA-PEPTIDE targets identified herein may be expressed by tumor cells in a population of human subjects. For example, the HLA-PEPTIDE targets identified herein may be shared antigens which are commonly expressed in a population of human subjects with cancer. 
     The HLA-PEPTIDE targets identified herein may have a prevalence with an individual tumor type The prevalence with an individual tumor type may be about 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95% 96%, 97%, 98%, 99%, or 100%. The prevalence with an individual tumor type may be about 0.1%-100%, 0.2-50%, 0.5-25%, or 1-10%. 
     Preferably, HLA-PEPTIDE targets are not generally expressed in most normal tissues. For example, the HLA-PEPTIDE targets may in some cases not be expressed in tissues in the Genotype-Tissue Expression (GTEx) Project, or may in some cases be expressed only in immune privileged or non-essential tissues. Exemplary immune privileged or non-essential tissues include testis, minor salivary glands, the endocervix, and the thyroid. In some cases, an HLA-PEPTIDE target may be deemed to not be expressed on essential tissues or non-immune privileged tissues if the median expression of a gene from which the restricted peptide is derived is less than 0.5 RPKM (Reads Per Kilobase of transcript per Million napped reads) across GTEx samples, if the gene is not expressed with greater than 10 RPKM across GTEX samples, if the gene was expressed at &gt;=5 RPKM in no more two samples across all essential tissue samples, or any combination thereof. 
     Exemplary HLA Class I Subtypes of the HLA-PEPTIDE Targets 
     In humans, there are many MHC haplotypes (referred to interchangeably herein as MHC subtypes, HLA subtypes, MHC types, and HLA types). Exemplary HLA subtypes include, by way of example only, HLA-A2, HLA-A1, HLA-A3, HLA-A11, HLA-A23, HLA-A24, HLA-A25, HLA-A26, HLA-A28, HLA-A29, HLA-A30, HLA-A31, HLA-A32, HLA-A33, HLA-A34, HLA-68, HLA-B7, HLA-B8, HLA-B40, HLA-B44, HLA-B13, HLA-B15, HLA-B-18, HLA-B27, HLA-B35, HLA-B37, HLA-B38, HLA-B39, HLA-B45, HLA-B46, HLA-B49, HLA-B51, HLA-B54, HLA-B55, HLA-B56, HLA-B57, HLA-B58, HLA-C*01, HLA-C*02, HLA-C*03, HLA-C*04, HLA-C*05, HLA-C*06, HLA-C*07, HLA-C*12, HLA-C*14, HLA-C*16, HLA-Cw8, HLA-A*01:01, HLA-A*02:01, HLA-A*02:03, HLA-A*02:04, HLA-A*02:07, HLA-A*03:01, HLA-A*03:02, HLA-A*11:01, HLA-A*23:01, HLA-A*24:02, HLA-A*25:01, HLA-A*26:01, HLA-A*29:02, HLA-A*30:01, HLA-A*30:02, HLA-A*31:01, HLA-A*32:01, HLA-A*33:01, HLA-A*33:03, HLA-A*68:01, HLA-A*68:02, HLA-B*07:02, HLA-B*08:01, HLA-B*13:02, HLA-B*15:01, HLA-B*15:03, HLA-B*18:01, HLA-B*27:02, HLA-B*27:05, HLA-B*35:01, HLA-B*35:03, HLA-B*37:01, HLA-B*38:01, HLA-B*39:01, HLA-B*40:01, HLA-B*40:02, HLA-B*44:02, HLA-B*44:03, HLA-B*46:01, HLA-B*49:01, HLA-B*51:01, HLA-B*54:01, HLA-B*55:01, HLA-B*56:01, HLA-B*57:01, HLA-B*58:01, HLA-C*01:02, HLA-C*02:02, HLA-C*03:03, HLA-C*03:04, HLA-C*04:01, HLA-C*05:01, HLA-C*06:02, HLA-C*07:01, HLA-C*07:02, HLA-C*07:04, HLA-C*07:06, HLA-C*12:03, HLA-C*14:02, HLA-C*16:01, HLA-C*16:02, HLA-C*16:04, and all subtypes thereof, including, e.g., 4 digit, 6 digit, and 8 digit subtypes. As is known to those skilled in the art there are allelic variants of the above HLA types, all of which are encompassed by the present invention. A full list of HLA Class Alleles can be found on http://hla.alleles.org/alleles/. For example, a full list of HLA Class I Alleles can be found on http://hla.alleles.org/alleles/class1.html. 
     HLA-Restricted Peptides 
     The HLA-restricted peptides (referred to interchangeably herein) as “restricted peptides” can be peptide fragments of tumor-specific genes, e.g., cancer-specific genes. Preferably, the cancer-specific genes are expressed in cancer samples. Genes which are aberrantly expressed in cancer samples can be identified through a database. Exemplary databases include, by way of example only, The Cancer Genome Atlas (TCGA) Research Network: http://cancergenome.nih.gov/; the International Cancer Genome Consortium: https://dcc.icgc.org/. In some embodiments, the cancer-specific gene has an observed expression of at least 10 RPKM in at least 5 samples from the TCGA database. The cancer-specific gene may have an observable bimodal distribution 
     The cancer-specific gene may have an observed expression of greater than 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 transcripts per million (TPM) in at least one TCGA tumor tissue. In preferred embodiments, the cancer-specific gene has an observed expression of greater than 100 TPM in at least one TCGA tumor tissue. In some cases, the cancer specific gene has an observed bimodal distribution of expression across TCGA samples. Without wishing to be bound by theory, such bimodal expression pattern is consistent with a biological model in which there is minimal expression at baseline in all tumor samples and higher expression in a subset of tumors experiencing epigenetic dysregulation. 
     Preferably, the cancer-specific gene is not generally expressed in most normal tissues. For example, the cancer-specific gene may in some cases not be expressed in tissues in the Genotype-Tissue Expression (GTEx) Project, or may in some cases be expressed in immune privileged or non-essential tissues. Exemplary immune privileged or non-essential tissues include testis, minor salivary glands, the endocervix, and thyroid. In some cases, an cancer-specific gene may be deemed to not be expressed an essential tissues or non-immune privileged tissue if the median expression of the cancer-specific gene is less than 0.5 RPKM (Reads Per Kilobase of transcript per Million napped reads) across GTEx samples, if the gene is not expressed with greater than 10 RPKM across GTEX samples, if the gene was expressed at &gt;=5 RPKM in no more two samples across all essential tissue samples, or any combination thereof. 
     In some embodiments, the cancer-specific gene meets the following criteria by assessment of the GTEx: (1) median GTEx expression in brain, heart, or lung is less than 0.1 transcripts per million (TPM), with no one sample exceeding 5 TPM, (2) median GTEx expression in other essential organs (excluding testis, thyroid, minor salivary gland) is less than 2 TPM with no one sample exceeding 10 TPM. 
     In some embodiments, the cancer-specific gene is not likely expressed in immune cells generally, e.g., is not an interferon family gene, is not an eye-related gene, not an olfactory or taste receptor gene, and is not a gene related to the circadian cycle (e.g., not a CLOCK, PERIOD, CRY gene) 
     The restricted peptide preferably may be presented on the surface of a tumor. 
     The restricted peptides may have a size of about 5, about 6, about 7, about 8, about 9, about 10, about 11, about 12, about 13, about 14, or about 15 amino molecule residues, and any range derivable therein. In particular embodiments, the restricted peptide has a size of about 8, about 9, about 10, about 11, or about 12 amino molecule residues. The restricted peptide may be about 5-15 amino acids in length, preferably may be about 7-12 amino acids in length, or more preferably may be about 8-11 amino acids in length. 
     Exemplary HLA-PEPTIDE Targets 
     Exemplary HLA-PEPTIDE targets are shown in Tables A, A1, and A2. In each row of Tables A, A1, and A2 the HLA allele and corresponding HLA-restricted peptide sequence of each complex is shown. The peptide sequence can consist of the respective sequence shown in any one of the rows of Tables A, A1, or A2. Alternatively the peptide sequence can comprise the respective sequence shown in any one of the rows of Tables A, A1, or A2. Alternatively the peptide sequence can consist essentially of the respective sequence shown in any one of the rows of Tables A, A1, or A2. 
     In some embodiments, the HLA-PEPTIDE target is a target as shown in Table A, A1, or A2. 
     In some embodiments, the HLA-PEPTIDE target is a target shown in Table A, A1, or A2, with the proviso that the isolated HLA-PEPTIDE target is not any one of Target nos. 6364-6369, 6386-6389, 6500, 6521-6524, or 6578 and is not an HLA-PEPTIDE target found in Table B or Table C. 
     In some embodiments, the HLA-restricted peptide is not from a gene selected from WT1 or MART1. 
     HLA Class I molecules which do not associate with a restricted peptide ligand are generally unstable. Accordingly, the association of the restricted peptide with the α1/α2 groove of the HLA molecule may stabilize the non-covalent association of the β2-microglobulin subunit of the HLA subtype with the α-subunit of the HLA subtype. 
     Stability of the non-covalent association of the β2-microglobulin subunit of the HLA subtype with the α-subunit of the HLA subtype can be determined using any suitable means. For example, such stability may be assessed by dissolving insoluble aggregates of HLA molecules in high concentrations of urea (e.g., about 8M urea), and determining the ability of the HLA molecule to refold in the presence of the restricted peptide during urea removal, e.g., urea removal by dialysis. Such refolding approaches are described in, e.g., Proc. Natl. Acad. Sci. USA Vol. 89, pp. 3429-3433, April 1992, hereby incorporated by reference. 
     For other example, such stability may be assessed using conditional HLA Class I ligands. Conditional HLA Class I ligands are generally designed as short restricted peptides which stabilize the association of the β2 and a subunits of the HLA Class I molecule by binding to the α1/α2 groove of the HLA molecule, and which contain one or more amino acid modifications allowing cleavage of the restricted peptide upon exposure to a conditional stimulus. Upon cleavage of the conditional ligand, the β2 and α-subunits of the HLA molecule dissociate, unless such conditional ligand is exchanged for a restricted peptide which binds to the α1/α2 groove and stabilizes the HLA molecule. Conditional ligands can be designed by introducing amino acid modifications in either known HLA peptide ligands or in predicted high-affinity HLA peptide ligands. For HLA alleles for which structural information is available, water-accessibility of side chains may also be used to select positions for introduction of the amino acid modifications. Use of conditional HLA ligands may be advantageous by allowing the batch preparation of stable HLA-peptide complexes which may be used to interrogate test restricted peptides in a high throughput manner. Conditional HLA Class I ligands, and methods of production, are described in, e.g., Proc Natl Acad Sci USA. 2008 Mar. 11; 105(10): 3831-3836; Proc Natl Acad Sci USA. 2008 Mar. 11; 105(10): 3825-3830; J Exp Med. 2018 May 7; 215(5): 1493-1504; Choo, J. A. L. et al. Bioorthogonal cleavage and exchange of major histocompatibility complex ligands by employing azobenzene-containing peptides. Angew Chem Int Ed Engl 53, 13390-13394 (2014); Amore, A. et al. Development of a Hypersensitive Periodate-Cleavable Amino Acid that is Methionine- and Disulfide-Compatible and its Application in MHC Exchange Reagents for T Cell Characterisation. ChemBioChem 14, 123-131 (2012); Rodenko, B. et al. Class I Major Histocompatibility Complexes Loaded by a Periodate Trigger. J Am Chem Soc 131, 12305-12313 (2009); and Chang, C. X. L. et al. Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases. Eur J Immunol 43, 1109-1120 (2013). These references are incorporated by reference in their entirety. 
     Accordingly, in some embodiments, the ability of an HLA-restricted peptide described herein, e.g., described in Table A, A1, or A2, to stabilize the association of the β2- and α-subunits of the HLA molecule, is assessed by performing a conditional ligand mediated-exchange reaction and assay for HLA stability. HLA stability can be assayed using any suitable method, including, e.g., mass spectrometry analysis, immunoassays (e.g., ELISA), size exclusion chromatography, and HLA multimer staining followed by flow cytometry assessment of T cells. 
     Other exemplary methods for assessing stability of the non-covalent association of the β2-microglobulin subunit of the HLA subtype with the α-subunit of the HLA subtype include peptide exchange using dipeptides. Peptide exchange using dipeptides has been described in, e.g., Proc Natl Acad Sci USA. 2013 Sep. 17, 110(38):15383-8; Proc Natl Acad Sci USA. 2015 Jan. 6, 112(1):202-7, which is hereby incorporated by reference. 
     Provided herein are useful antigens comprising an HLA-PEPTIDE target. The HLA-PEPTIDE targets may comprise a specific HLA-restricted peptide having a defined amino acid sequence complexed with a specific HLA subtype allele. 
     The HLA-PEPTIDE target may be isolated and/or in substantially pure form. For example, the HLA-PEPTIDE targets may be isolated from their natural environment, or may be produced by means of a technical process. In some cases, the HLA-PEPTIDE target is provided in a form which is substantially free of other peptides or proteins. 
     THE HLA-PEPTIDE targets may be presented in soluble form, and optionally may be a recombinant HLA-PEPTIDE target complex. The skilled artisan may use any suitable method for producing and purifying recombinant HLA-PEPTIDE targets. Suitable methods include, e.g., use of  E. coli  expression systems, insect cells, and the like. Other methods include synthetic production, e.g., using cell free systems. An exemplary suitable cell free system is described in WO2017089756, which is hereby incorporated by reference in its entirety. 
     Also provided herein are compositions comprising an HLA-PEPTIDE target. 
     In some cases, the composition comprises an HLA-PEPTIDE target attached to a solid support. Exemplary solid supports include, but are not limited to, beads, wells, membranes, tubes, columns, plates, sepharose, magnetic beads, and chips. Exemplary solid supports are described in, e.g., Catalysts 2018, 8, 92; doi:10.3390/cata18020092, which is hereby incorporated by reference in its entirety. 
     The HLA-PEPTIDE target may be attached to the solid support by any suitable methods known in the art. In some cases, the HLA-PEPTIDE target is covalently attached to the solid support. 
     In some cases, the HLA-PEPTIDE target is attached to the solid support by way of an affinity binding pair. Affinity binding pairs generally involved specific interactions between two molecules. A ligand having an affinity for its binding partner molecule can be covalently attached to the solid support, and thus used as bait for immobilizing Common affinity binding pairs include, e.g., streptavidin and biotin, avidin and biotin; polyhistidine tags with metal ions such as copper, nickel, zinc, and cobalt; and the like. 
     The HLA-PEPTIDE target may comprise a detectable label. 
     Pharmaceutical compositions comprising HLA-PEPTIDE targets. 
     The composition comprising an HLA-PEPTIDE target may be a pharmaceutical composition. Such a composition may comprise multiple HLA-PEPTIDE targets. Exemplary pharmaceutical compositions are described herein. The composition may be capable of eliciting an immune response. The composition may comprise an adjuvant. Suitable adjuvants include, but are not limited to 1018 ISS, alum, aluminium salts, Amplivax, AS15, BCG, CP-870,893, CpG7909, CyaA, dSLIM, GM-CSF, IC30, IC31, Imiquimod, ImuFact IMP321, IS Patch, ISS, ISCOMATRIX, JuvImmune, LipoVac, MF59, monophosphoryl lipid A, Montanide IMS 1312, Montanide ISA 206, Montanide ISA 50V, Montanide ISA-51, OK-432, OM-174, OM-197-MP-EC, ONTAK, PepTel vector system, PLG microparticles, resiquimod, SRL172, Virosomes and other Virus-like particles, YF-17D, VEGF trap, R848, beta-glucan, Pam3Cys, Aquila&#39;s QS21 stimulon (Aquila Biotech, Worcester, Mass., USA) which is derived from saponin, mycobacterial extracts and synthetic bacterial cell wall mimics, and other proprietary adjuvants such as Ribi&#39;s Detox. Quil or Superfos. Adjuvants such as incomplete Freund&#39;s or GM-CSF are useful. Several immunological adjuvants (e.g., MF59) specific for dendritic cells and their preparation have been described previously (Dupuis M, et al., Cell Immunol. 1998; 186(1):18-27; Allison A C; Dev Biol Stand. 1998; 92:3-11). Also cytokines can be used. Several cytokines have been directly linked to influencing dendritic cell migration to lymphoid tissues (e.g., TNF-alpha), accelerating the maturation of dendritic cells into efficient antigen-presenting cells for T-lymphocytes (e.g., GM-CSF, IL-1 and IL-4) (U.S. Pat. No. 5,849,589, specifically incorporated herein by reference in its entirety) and acting as immunoadjuvants (e.g., IL-12) (Gabrilovich D I, et al., J Immunother Emphasis Tumor Immunol. 1996 (6):414-418). HLA surface expression and processing of intracellular proteins into peptides to present on HLA can also be enhanced by interferon-gamma (IFN-γ). See, e.g., York I A, Goldberg A L, Mo X Y, Rock K L. Proteolysis and class I major histocompatibility complex antigen presentation. Immunol Rev. 1999; 172:49-66; and Rock K L, Goldberg A L. Degradation of cell proteins and the generation of MHC class I-presented peptides. Ann Rev Immunol. 1999; 17: 12. 739-779, which are incorporated herein by reference in their entirety. 
     HLA-Peptide ABPs 
     Also provided herein are ABPs that specifically bind to HLA-PEPTIDE target as disclosed herein. 
     The HLA-PEPTIDE target may be expressed on the surface of any suitable target cell including a tumor cell. 
     The ABP can specifically bind to a human leukocyte antigen (HLA)-PEPTIDE target, wherein the HLA-PEPTIDE target comprises an HLA-restricted peptide complexed with an HLA Class I molecule, wherein the HLA-restricted peptide is located in the peptide binding groove of an α1/α2 heterodimer portion of the HLA Class I molecule. 
     In some aspects, the ABP does not bind HLA class I in the absence of HLA-restricted peptide. In some aspects, the ABP does not bind HLA-restricted peptide in the absence of human MHC class I. In some aspects, the ABP binds tumor cells presenting human MHC class I being complexed with HLA-restricted peptide, optionally wherein the HLA restricted peptide is a tumor antigen characterizing the cancer. 
     An ABP can bind to each portion of an HLA-PEPTIDE complex (i.e., HLA and peptide representing each portion of the complex), which when bound together form a novel target and protein surface for interaction with and binding by the ABP, distinct from a surface presented by the peptide alone or HLA subtype alone. Generally the novel target and protein surface formed by binding of HLA to peptide does not exist in the absence of each portion of the HLA-PEPTIDE complex. 
     An ABP can be capable of specifically binding a complex comprising HLA and an HLA-restricted peptide (HLA-PEPTIDE), e.g., derived from a tumor. In some aspects, the ABP does not bind HLA in an absence of the HLA-restricted peptide derived from the tumor. In some aspects, the ABP does not bind the HLA-restricted peptide derived from the tumor in an absence of HLA. In some aspects, the ABP binds a complex comprising HLA and HLA-restricted peptide when naturally presented on a cell such as a tumor cell. 
     In some embodiments, an ABP provided herein modulates binding of HLA-PEPTIDE to one or more ligands of HLA-PEPTIDE. 
     The ABP may specifically bind to any one of the HLA-PEPTIDE targets as disclosed in Table A, A1, or A2. In some embodiments, the HLA-restricted peptide is not from a gene selected from WT1 or MART1. In some embodiments, the ABP does not specifically bind to any one of Target nos. 6364-6369, 6386-6389, 6500, 6521-6524, or 6578 and does not specifically bind to an HLA-PEPTIDE target found in Table B or Table C. 
     In more particular embodiments, the ABP specifically binds to an HLA-PEPTIDE target selected from any one of: HLA subtype A*02:01 complexed with an HLA-restricted peptide comprising the sequence LLASSILCA, HLA subtype A*01:01 complexed with an HLA-restricted peptide comprising the sequence EVDPIGHLY, HLA subtype B*44:02 complexed with an HLA-restricted peptide comprising the sequence GEMSSNSTAL, HLA subtype A*02:01 complexed with an HLA-restricted peptide comprising the sequence GVYDGEEHSV, HLA subtype *01:01 complexed with an HLA-restricted peptide comprising the sequence EVDPIGHVY, HLA subtype HLA-A*01:01 complexed with an HLA-restricted peptide comprising the sequence NTDNNLAVY, HLA subtype B*35:01 complexed with an HLA-restricted peptide comprising the sequence EVDPIGHVY, HLA subtype A*02:01 complexed with an HLA-restricted peptide comprising the sequence AIFPGAVPAA, and HLA subtype A*01:01 complexed with an HLA-restricted peptide comprising the sequence ASSLPTTMNY. 
     In more particular embodiments, the ABP specifically binds to an HLA-PEPTIDE target selected from any one of: HLA subtype A*02:01 complexed with an HLA-restricted peptide consisting essentially of the sequence LLASSILCA, HLA subtype A*01:01 complexed with an HLA-restricted peptide consisting essentially of the sequence EVDPIGHLY, HLA subtype B*44:02 complexed with an HLA-restricted peptide consisting essentially of the sequence GEMSSNSTAL, HLA subtype A*02:01 complexed with an HLA-restricted peptide consisting essentially of the sequence GVYDGEEHSV, HLA subtype *01:01 complexed with an HLA-restricted peptide consisting essentially of the sequence EVDPIGHVY, HLA subtype HLA-A*01:01 complexed with an HLA-restricted peptide consisting essentially of the sequence NTDNNLAVY, HLA subtype B*35:01 complexed with an HLA-restricted peptide consisting essentially of the sequence EVDPIGHVY, HLA subtype A*02:01 complexed with an HLA-restricted peptide consisting essentially of the sequence AIFPGAVPAA, and HLA subtype A*01:01 complexed with an HLA-restricted peptide consisting essentially of the sequence ASSLPTTMNY. 
     In more particular embodiments, the ABP specifically binds to an HLA-PEPTIDE target selected from any one of: HLA subtype A*02:01 complexed with an HLA-restricted peptide consisting of the sequence LLASSILCA, HLA subtype A*01:01 complexed with an HLA-restricted peptide consisting of the sequence EVDPIGHLY, HLA subtype B*44:02 complexed with an HLA-restricted peptide consisting of the sequence GEMSSNSTAL, HLA subtype A*02:01 complexed with an HLA-restricted peptide consisting of the sequence GVYDGEEHSV, HLA subtype *01:01 complexed with an HLA-restricted peptide consisting of the sequence EVDPIGHVY, HLA subtype HLA-A*01:01 complexed with an HLA-restricted peptide consisting of the sequence NTDNNLAVY, HLA subtype B*35:01 complexed with an HLA-restricted peptide consisting of the sequence EVDPIGHVY, HLA subtype A*02:01 complexed with an HLA-restricted peptide consisting of the sequence AIFPGAVPAA, and HLA subtype A*01:01 complexed with an HLA-restricted peptide consisting of the sequence ASSLPTTMNY. 
     In some embodiments, an ABP is an ABP that competes with an illustrative ABP provided herein. In some aspects, the ABP that competes with the illustrative ABP provided herein binds the same epitope as an illustrative ABP provided herein. 
     In some embodiments, the ABPs described herein are referred to herein as “variants.” In some embodiments, such variants are derived from a sequence provided herein, for example, by affinity maturation, site directed mutagenesis, random mutagenesis, or any other method known in the art or described herein. In some embodiments, such variants are not derived from a sequence provided herein and may, for example, be isolated de novo according to the methods provided herein for obtaining ABPs. In some embodiments, a variant is derived from any of the sequences provided herein, wherein one or more conservative amino acid substitutions are made. In some embodiments, a variant is derived from any of the sequences provided herein, wherein one or more nonconservative amino acid substitutions are made. Conservative amino acid substitutions are described herein. Exemplary nonconservative amino acid substitutions include those described in J Immunol. 2008 May 1; 180(9):6116-31, which is hereby incorporated by reference in its entirety. In preferred embodiments, the non-conservative amino acid substitution does not interfere with or inhibit the biological activity of the functional variant. In yet more preferred embodiments, the non-conservative amino acid substitution enhances the biological activity of the functional variant, such that the biological activity of the functional variant is increased as compared to the parent ABP. 
     ABPs Comprising an Antibody or Antigen-Binding Fragment Thereof 
     An ABP may comprise an antibody or antigen-binding fragment thereof. 
     In some embodiments, the ABPs provided herein comprise a light chain. In some aspects, the light chain is a kappa light chain. In some aspects, the light chain is a lambda light chain. 
     In some embodiments, the ABPs provided herein comprise a heavy chain. In some aspects, the heavy chain is an IgA. In some aspects, the heavy chain is an IgD. In some aspects, the heavy chain is an IgE. In some aspects, the heavy chain is an IgG. In some aspects, the heavy chain is an IgM. In some aspects, the heavy chain is an IgG1. In some aspects, the heavy chain is an IgG2. In some aspects, the heavy chain is an IgG3. In some aspects, the heavy chain is an IgG4. In some aspects, the heavy chain is an IgA1. In some aspects, the heavy chain is an IgA2. 
     In some embodiments, the ABPs provided herein comprise an antibody fragment. In some embodiments, the ABPs provided herein consist of an antibody fragment. In some embodiments, the ABPs provided herein consist essentially of an antibody fragment. In some aspects, the ABP fragment is an Fv fragment. In some aspects, the ABP fragment is a Fab fragment. In some aspects, the ABP fragment is a F(ab′)2 fragment. In some aspects, the ABP fragment is a Fab′ fragment. In some aspects, the ABP fragment is an scFv (sFv) fragment. In some aspects, the ABP fragment is an scFv-Fc fragment. In some aspects, the ABP fragment is a fragment of a single domain ABP. 
     In some embodiments, an ABP fragment provided herein is derived from an illustrative ABP provided herein. In some embodiments, an ABP fragments provided herein is not derived from an illustrative ABP provided herein and may, for example, be isolated de novo according to the methods provided herein for obtaining ABP fragments. 
     In some embodiments, an ABP fragment provided herein retains the ability to bind the HLA-PEPTIDE target, as measured by one or more assays or biological effects described herein. In some embodiments, an ABP fragment provided herein retains the ability to prevent HLA-PEPTIDE from interacting with one or more of its ligands, as described herein. 
     In some embodiments, the ABPs provided herein are monoclonal ABPs. In some embodiments, the ABPs provided herein are polyclonal ABPs. 
     In some embodiments, the ABPs provided herein comprise a chimeric ABP. In some embodiments, the ABPs provided herein consist of a chimeric ABP. In some embodiments, the ABPs provided herein consist essentially of a chimeric ABP. In some embodiments, the ABPs provided herein comprise a humanized ABP. In some embodiments, the ABPs provided herein consist of a humanized ABP. In some embodiments, the ABPs provided herein consist essentially of a humanized ABP. In some embodiments, the ABPs provided herein comprise a human ABP. In some embodiments, the ABPs provided herein consist of a human ABP. In some embodiments, the ABPs provided herein consist essentially of a human ABP. 
     In some embodiments, the ABPs provided herein comprise an alternative scaffold. In some embodiments, the ABPs provided herein consist of an alternative scaffold. In some embodiments, the ABPs provided herein consist essentially of an alternative scaffold. Any suitable alternative scaffold may be used. In some aspects, the alternative scaffold is selected from an Adnectin™, an iMab, an Anticalin®, an EETI-II/AGRP, a Kunitz domain, a thioredoxin peptide aptamer, an Affibody®, a DARPin, an Affilin, a Tetranectin, a Fynomer, and an Avimer. 
     Also disclosed herein is an isolated humanized, human, or chimeric ABP that competes for binding to an HLA-PEPTIDE with an ABP disclosed herein. 
     Also disclosed herein is an isolated humanized, human, or chimeric ABP that binds an HLA-PEPTIDE epitope bound by an ABP disclosed herein. 
     In certain aspects, an ABP comprises a human Fc region comprising at least one modification that reduces binding to a human Fc receptor. 
     It is known that when an ABP is expressed in cells, the ABP is modified after translation. Examples of the posttranslational modification include cleavage of lysine at the C terminus of the heavy chain by a carboxypeptidase; modification of glutamine or glutamic acid at the N terminus of the heavy chain and the light chain to pyroglutamic acid by pyroglutamylation; glycosylation; oxidation; deamidation; and glycation, and it is known that such posttranslational modifications occur in various ABPs (See Journal of Pharmaceutical Sciences, 2008, Vol. 97, p. 2426-2447, incorporated by reference in its entirety). In some embodiments, an ABP is an ABP or antigen-binding fragment thereof which has undergone posttranslational modification. Examples of an ABP or antigen-binding fragment thereof which have undergone posttranslational modification include an ABP or antigen-binding fragments thereof which have undergone pyroglutamylation at the N terminus of the heavy chain variable region and/or deletion of lysine at the C terminus of the heavy chain. It is known in the art that such posttranslational modification due to pyroglutamylation at the N terminus and deletion of lysine at the C terminus does not have any influence on the activity of the ABP or fragment thereof (Analytical Biochemistry, 2006, Vol. 348, p. 24-39, incorporated by reference in its entirety). 
     Monospecific and Multispecific HLA-PEPTIDE ABPs 
     In some embodiments, the ABPs provided herein are monospecific ABPs. 
     In some embodiments, the ABPs provided herein are multispecific ABPs. 
     In some embodiments, a multispecific ABP provided herein binds more than one antigen. In some embodiments, a multispecific ABP binds 2 antigens. In some embodiments, a multispecific ABP binds 3 antigens. In some embodiments, a multispecific ABP binds 4 antigens. In some embodiments, a multispecific ABP binds 5 antigens. 
     In some embodiments, a multispecific ABP provided herein binds more than one epitope on a HLA-PEPTIDE antigen. In some embodiments, a multispecific ABP binds 2 epitopes on a HLA-PEPTIDE antigen. In some embodiments, a multispecific ABP binds 3 epitopes on a HLA-PEPTIDE antigen. 
     Many multispecific ABP constructs are known in the art, and the ABPs provided herein may be provided in the form of any suitable multispecific suitable construct. 
     In some embodiments, the multispecific ABP comprises an immunoglobulin comprising at least two different heavy chain variable regions each paired with a common light chain variable region (i.e., a “common light chain ABP”). The common light chain variable region forms a distinct antigen-binding domain with each of the two different heavy chain variable regions. See Merchant et al.,  Nature Biotechnol.,  1998, 16:677-681, incorporated by reference in its entirety. 
     In some embodiments, the multispecific ABP comprises an immunoglobulin comprising an ABP or fragment thereof attached to one or more of the N- or C-termini of the heavy or light chains of such immunoglobulin. See Coloma and Morrison, Nature  Biotechnol.,  1997, 15:159-163, incorporated by reference in its entirety. In some aspects, such ABP comprises a tetravalent bispecific ABP. 
     In some embodiments, the multispecific ABP comprises a hybrid immunoglobulin comprising at least two different heavy chain variable regions and at least two different light chain variable regions. See Milstein and Cuello,  Nature,  1983, 305:537-540; and Staerz and Bevan,  Proc. Natl. Acad. Sci. USA,  1986, 83:1453-1457; each of which is incorporated by reference in its entirety. 
     In some embodiments, the multispecific ABP comprises immunoglobulin chains with alterations to reduce the formation of side products that do not have multispecificity. In some aspects, the ABPs comprise one or more “knobs-into-holes” modifications as described in U.S. Pat. No. 5,731,168, incorporated by reference in its entirety. 
     In some embodiments, the multispecific ABP comprises immunoglobulin chains with one or more electrostatic modifications to promote the assembly of Fc hetero-multimers. See WO 2009/089004, incorporated by reference in its entirety. 
     In some embodiments, the multispecific ABP comprises a bispecific single chain molecule. See Traunecker et al.,  EMBO J.,  1991, 10:3655-3659; and Gruber et al., J. Immunol., 1994, 152:5368-5374; each of which is incorporated by reference in its entirety. 
     In some embodiments, the multispecific ABP comprises a heavy chain variable domain and a light chain variable domain connected by a polypeptide linker, where the length of the linker is selected to promote assembly of multispecific ABP with the desired multispecificity. For example, monospecific scFvs generally form when a heavy chain variable domain and light chain variable domain are connected by a polypeptide linker of more than 12 amino acid residues. See U.S. Pat. Nos. 4,946,778 and 5,132,405, each of which is incorporated by reference in its entirety. In some embodiments, reduction of the polypeptide linker length to less than 12 amino acid residues prevents pairing of heavy and light chain variable domains on the same polypeptide chain, thereby allowing pairing of heavy and light chain variable domains from one chain with the complementary domains on another chain. The resulting ABP therefore has multispecificity, with the specificity of each binding site contributed by more than one polypeptide chain. Polypeptide chains comprising heavy and light chain variable domains that are joined by linkers between 3 and 12 amino acid residues form predominantly dimers (termed diabodies). With linkers between 0 and 2 amino acid residues, trimers (termed triabodies) and tetramers (termed tetrabodies) are favored. However, the exact type of oligomerization appears to depend on the amino acid residue composition and the order of the variable domain in each polypeptide chain (e.g., V H -linker-V L  vs. V L -linker-V H ), in addition to the linker length. A skilled person can select the appropriate linker length based on the desired multispecificity. 
     Fc Region and Variants 
     In certain embodiments, an ABP provided herein comprises an Fc region. An Fc region can be wild-type or a variant thereof. In certain embodiments, an ABP provided herein comprises an Fc region with one or more amino acid substitutions, insertions, or deletions in comparison to a naturally occurring Fc region. In some aspects, such substitutions, insertions, or deletions yield ABP with altered stability, glycosylation, or other characteristics. In some aspects, such substitutions, insertions, or deletions yield a glycosylated ABP. 
     A “variant Fc region” or “engineered Fc region” comprises an amino acid sequence that differs from that of a native-sequence Fc region by virtue of at least one amino acid modification, preferably one or more amino acid substitution(s). Preferably, the variant Fc region has at least one amino acid substitution compared to a native-sequence Fc region or to the Fc region of a parent polypeptide, e.g., from about one to about ten amino acid substitutions, and preferably from about one to about five amino acid substitutions in a native-sequence Fc region or in the Fc region of the parent polypeptide. The variant Fc region herein will preferably possess at least about 80% homology with a native-sequence Fc region and/or with an Fc region of a parent polypeptide, and most preferably at least about 90% homology therewith, more preferably at least about 95% homology therewith. 
     The term “Fc-region-comprising ABP” refers to an ABP that comprises an Fc region. The C-terminal lysine (residue 447 according to the EU numbering system) of the Fc region may be removed, for example, during purification of the ABP or by recombinant engineering the nucleic acid encoding the ABP. Accordingly, an ABP having an Fc region can comprise an ABP with or without K447. 
     In some aspects, the Fc region of an ABP provided herein is modified to yield an ABP with altered affinity for an Fc receptor, or an ABP that is more immunologically inert. In some embodiments, the ABP variants provided herein possess some, but not all, effector functions. Such ABPs may be useful, for example, when the half-life of the ABP is important in vivo, but when certain effector functions (e.g., complement activation and ADCC) are unnecessary or deleterious. 
     In some embodiments, the Fc region of an ABP provided herein is a human IgG4 Fc region comprising one or more of the hinge stabilizing mutations S228P and L235E. See Aalberse et al.,  Immunology,  2002, 105:9-19, incorporated by reference in its entirety. In some embodiments, the IgG4 Fc region comprises one or more of the following mutations: E233P, F234V, and L235A. See Armour et al.,  Mol. Immunol.,  2003, 40:585-593, incorporated by reference in its entirety. In some embodiments, the IgG4 Fc region comprises a deletion at position G236. 
     In some embodiments, the Fc region of an ABP provided herein is a human IgG1 Fc region comprising one or more mutations to reduce Fc receptor binding. In some aspects, the one or more mutations are in residues selected from S228 (e.g., S228A), L234 (e.g., L234A), L235 (e.g., L235A), D265 (e.g., D265A), and N297 (e.g., N297A). In some aspects, the ABP comprises a PVA236 mutation. PVA236 means that the amino acid sequence ELLG, from amino acid position 233 to 236 of IgG1 or EFLG of IgG4, is replaced by PVA. See U.S. Pat. No. 9,150,641, incorporated by reference in its entirety. 
     In some embodiments, the Fc region of an ABP provided herein is modified as described in Armour et al.,  Eur. J. Immunol.,  1999, 29:2613-2624; WO 1999/058572; and/or U.K. Pat. App. No. 98099518; each of which is incorporated by reference in its entirety. 
     In some embodiments, the Fc region of an ABP provided herein is a human IgG2 Fc region comprising one or more of mutations A330S and P331S. 
     In some embodiments, the Fc region of an ABP provided herein has an amino acid substitution at one or more positions selected from 238, 265, 269, 270, 297, 327 and 329. See U.S. Pat. No. 6,737,056, incorporated by reference in its entirety. Such Fc mutants include Fc mutants with substitutions at two or more of amino acid positions 265, 269, 270, 297 and 327, including the so-called “DANA” Fc mutant with substitution of residues 265 and 297 with alanine. See U.S. Pat. No. 7,332,581, incorporated by reference in its entirety. In some embodiments, the ABP comprises an alanine at amino acid position 265. In some embodiments, the ABP comprises an alanine at amino acid position 297. 
     In certain embodiments, an ABP provided herein comprises an Fc region with one or more amino acid substitutions which improve ADCC, such as a substitution at one or more of positions 298, 333, and 334 of the Fc region. In some embodiments, an ABP provided herein comprises an Fc region with one or more amino acid substitutions at positions 239, 332, and 330, as described in Lazar et al.,  Proc. Natl. Acad. Sci. USA,  2006, 103:4005-4010, incorporated by reference in its entirety. 
     In some embodiments, an ABP provided herein comprises one or more alterations that improves or diminishes C1q binding and/or CDC. See U.S. Pat. No. 6,194,551; WO 99/51642; and Idusogie et al.,  J. Immunol.,  2000, 164:4178-4184; each of which is incorporated by reference in its entirety. 
     In some embodiments, an ABP provided herein comprises one or more alterations to increase half-life. ABPs with increased half-lives and improved binding to the neonatal Fc receptor (FcRn) are described, for example, in Hinton et al.,  J. Immunol.,  2006, 176:346-356; and U.S. Pat. Pub. No. 2005/0014934; each of which is incorporated by reference in its entirety. Such Fc variants include those with substitutions at one or more of Fc region residues: 238, 250, 256, 265, 272, 286, 303, 305, 307, 311, 312, 314, 317, 340, 356, 360, 362, 376, 378, 380, 382, 413, 424, 428, and 434 of an IgG. In some embodiments, the ABP comprises one or more non-Fc modifications that extend half-life. Exemplary non-Fc modifications that extend half-life are described in, e.g., US20170218078, which is hereby incorporated by reference in its entirety. 
     In some embodiments, an ABP provided herein comprises one or more Fc region variants as described in U.S. Pat. Nos. 7,371,826 5,648,260, and 5,624,821; Duncan and Winter,  Nature,  1988, 322:738-740; and WO 94/29351; each of which is incorporated by reference in its entirety. 
     Antibodies Specific for B*35:01 EVDPIGHVY (HLA-PEPTIDE Target “G5”) 
     In some aspects, provided herein are ABPs comprising antibodies or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype B*35:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises, consists of, or essentially consists of the sequence EVDPIGHVY (“G5”). 
     CDRs 
     The ABP specific for B*35:01_EVDPIGHVY may comprise one or more antibody complementarity determining region (CDR) sequences, e.g., may comprise three heavy chain CDRs (CDR-H1, CDR-H2, CDR-H3) and three light chain CDRs (CDR-L1, CDR-L2, CDR-L3). 
     The ABP specific for B*35:01_EVDPIGHVY may comprise a CDR-H3 sequence. The CDR-H3 sequence may be selected from CARDGVRYYGMDVW, CARGVRGYDRSAGYW, CASHDYGDYGEYFQHW, CARVSWYCSSTSCGVNWFDPW, CAKVNWNDGPYFDYW, CATPTNSGYYGPYYYYGMDVW, CARDVMDVW, CAREGYGMDVW, CARDNGVGVDYW, CARGIADSGSYYGNGRDYYYGMDVW, CARGDYYFDYW, CARDGTRYYGMDVW, CARDVVANFDYW, CARGHSSGWYYYYGMDVW, CAKDLGSYGGYYW, CARSWFGGFNYHYYGMDVW, CARELPIGYGMDVW, and CARGGSYYYYGMDVW. 
     The ABP specific for B*35:01_EVDPIGHVY may comprise a CDR-L3 sequence. The CDR-L3 sequence may be selected from CMQGLQTPITF, CMQALQTPPTF, CQQAISFPLTF, CQQANSFPLTF, CQQANSFPLTF, CQQSYSIPLTF, CQQTYMMPYTF, CQQSYITPWTF, CQQSYITPYTF, CQQYYTTPYTF, CQQSYSTPLTF, CMQALQTPLTF, CQQYGSWPRTF, CQQSYSTPVTF, CMQALQTPYTF, CQQANSFPFTF, CMQALQTPLTF, and CQQSYSTPLTF. 
     The ABP specific for B*35:01_EVDPIGHVY may comprise a particular heavy chain CDR3 (CDR-H3) sequence and a particular light chain CDR3 (CDR-L3) sequence. In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G5_P7_E7, G5_P7_B3, G5_P7_A5, G5_P7_F6, G5-P1B12, G5-P1C12, G5-P1-E05, G5-P3G01, G5-P3G08, G5-P4B02, G5-P4E04, G5R4-P1D06, G5R4-P1H11, G5R4-P2B10, G5R4-P2H8, G5R4-P3G05, G5R4-P4A07, or G5R4-P4B01. CDR sequences of identified scFvs that specifically bind B*35:01_EVDPIGHVY are shown in Table 5. For clarity, each identified scFv hit is designated a clone name, and each row contains the CDR sequences for that particular clone name. For example, the scFv identified by clone name G5_P7_E7 comprises the heavy chain CDR3 sequence CARDGVRYYGMDVW and the light chain CDR3 sequence CMQGLQTPITF. 
     The ABP specific for B*35:01_EVDPIGHVY may comprise all six CDRs from the scFv designated G5_P7_E7, G5_P7_B3, G5_P7_A5, G5_P7_F6, G5-P1B12, G5-P1C12, G5-P1-E05, G5-P3G01, G5-P3G08, G5-P4B02, G5-P4E04, G5R4-P1D06, G5R4-P1H11, G5R4-P2B10, G5R4-P2H8, G5R4-P3G05, G5R4-P4A07, or G5R4-P4B01. 
     VH 
     The ABP specific for B*35:01_EVDPIGHVY may comprise a VH sequence. The VH sequence may be selected from 
     
       
         
           
               
            
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYDINWVRQAPGQGLEWMGI 
               
               
                   
               
               
                 INPRSGSTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDG 
               
               
                   
               
               
                 VRYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGYTFTSHDINWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 MNPNSGDTGYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARGV 
               
               
                   
               
               
                 RGYDRSAGYWGQGTLVIVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVKPGGSLRLSCAASGFSFSSYWMSWVRQAPGKGLEWISY 
               
               
                   
               
               
                 ISGDSGYTNYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCASHD 
               
               
                   
               
               
                 YGDYGEYFQHWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLQSGGGLVQPGGSLRLSCAASGFTFSNSDMNWVRQAPGKGLEWVAY 
               
               
                   
               
               
                 ISSGSSTIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARVS 
               
               
                   
               
               
                 WYCSSTSCGVNWFDPWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFSNSDMNWVRQAPGKGLEWVAS 
               
               
                   
               
               
                 ISSSGGYINYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKVN 
               
               
                   
               
               
                 WNDGPYFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGGTFSNFGVSWLRQAPGQGLEWMGG 
               
               
                   
               
               
                 IIPILGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCATPT 
               
               
                   
               
               
                 NSGYYGPYYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYNMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDV 
               
               
                   
               
               
                 MDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSGYLVSWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPNSGGTNTAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREG 
               
               
                   
               
               
                 YGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYIFRNYPMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPDSGGTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDN 
               
               
                   
               
               
                 GVGVDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 MNPNIGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGI 
               
               
                   
               
               
                 ADSGSYYGNGRDYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYGISWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPNSGVTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGD 
               
               
                   
               
               
                 YYFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYDINWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 INPNSGDTKYSQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDG 
               
               
                   
               
               
                 TRYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVKPGGSLRLSCAASGFTFSDYYMSWVRQAPGKGLEWVSY 
               
               
                   
               
               
                 ISSSSSYTNYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCARDV 
               
               
                   
               
               
                 VANFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 MNPDSGSTGYAQRFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGH 
               
               
                   
               
               
                 SSGWYYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFTSYSMHWVRQAPGKGLEWVSS 
               
               
                   
               
               
                 ITSFTNTMYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKDL 
               
               
                   
               
               
                 GSYGGYYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYMHWVRQAPGQGLEWMGI 
               
               
                   
               
               
                 INPSGGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARSW 
               
               
                   
               
               
                 FGGFNYHYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGW 
               
               
                   
               
               
                 MNPNSGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREL 
               
               
                   
               
               
                 PIGYGMDVWGQGTTVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGG 
               
               
                   
               
               
                 IIPIVGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARGG 
               
               
                   
               
               
                 SYYYYGMDVWGQGTTVTVSS. 
               
            
           
         
       
     
     VL 
     The ABP specific for B*35:01_EVDPIGHVY may comprise a VL sequence. The VL sequence may be selected from 
     
       
         
           
               
            
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSYRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGLQTP 
               
               
                   
               
               
                 ITFGQGTRLEIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSSRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 PTFGPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQAISFPLTFGQ 
               
               
                   
               
               
                 STKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYS 
               
               
                   
               
               
                 ASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSIPLTFGG 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLNWYQQKPGKAPKLLIYY 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQTYMMPYTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKWYGAS 
               
               
                   
               
               
                 SLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYITPWTFGQGT 
               
               
                   
               
               
                 KVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYITPYTFGQ 
               
               
                   
               
               
                 GTKLEIK, 
               
               
                   
               
               
                 DIVMTQSPDSLAVSLGERATINCKTSQSVLYRPNNENYLAWYQQKPGQPP 
               
               
                   
               
               
                 KLLIYQASIREPGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYTT 
               
               
                   
               
               
                 PYTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRFLNWYQQKPGKAPKLLIYG 
               
               
                   
               
               
                 ASRPQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSHRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 LTFGGGTKVEIK, 
               
               
                   
               
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVSSNLAWYQQKPGQAPRLLIYA 
               
               
                   
               
               
                 ASARASGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYGSWPRTFGQ 
               
               
                   
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKWYGAS 
               
               
                   
               
               
                 RLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPVTFGQGT 
               
               
                   
               
               
                 KVEIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 YTFGQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASEDISNHLNWYQQKPGKAPKLLIYD 
               
               
                   
               
               
                 ALSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPFTFGP 
               
               
                   
               
               
                 GTKVDIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQTP 
               
               
                   
               
               
                 LTFGQGTKVEIK, 
               
               
                 and 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTFGG 
               
               
                   
               
               
                 GTKVEIK. 
               
            
           
         
       
     
     VH-VL Combinations 
     The ABP specific for B*35:01_EVDPIGHVY may comprise a particular VH sequence and a particular VL sequence. In some embodiments, the ABP specific for B*35:01_EVDPIGHVY comprises a VH sequence and VL sequence from the scFv designated G5_P7_E7, G5_P7_B3, G5_P7_A5, G5_P7_F6, G5-P1B12, G5-P1C12, G5-P1-E05, G5-P3G01, G5-P3G08, G5-P4B02, G5-P4E04, G5R4-P1D06, G5R4-P1H11, G5R4-P2B10, G5R4-P2H8, G5R4-P3G05, G5R4-P4A07, or G5R4-P4B01. The VH and VL sequences of identified scFvs that specifically bind B*35:01_EVDPIGHVY are shown in Table 4. For clarity, each identified scFv hit is designated a clone name, and each row contains the VH and VL sequences for that particular clone name. For example, the scFv identified by clone name G5_P7_E7 comprises the VH sequence 
                    QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYDMWVRQAPGQGLEWMGII               NPRSGSTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDGV               RYYGMDVWGQGTTVTVSS            
and the VL sequence
 
     
       
         
           
               
            
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                   
               
               
                 LLIYLGSYRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGLQTP 
               
               
                   
               
               
                 ITFGQGTRLEIK. 
               
            
           
         
       
     
     Antibodies Specific for a*02:01 AIFPGAVPAA (HLA-PEPTIDE Target “G8”) 
     In some aspects, provided herein are ABPs comprising antibodies or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*02:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises, consists of, or essentially consists of the sequence AIFPGAVPAA (“G8”). 
     CDRs 
     The ABP specific for A*02:01_AIFPGAVPAA may comprise one or more antibody complementarity determining region (CDR) sequences, e.g., may comprise three heavy chain CDRs (CDR-H1, CDR-H2, CDR-H3) and three light chain CDRs (CDR-L1, CDR-L2, CDR-L3). 
     The ABP specific for A*02:01_AIFPGAVPAA may comprise a CDR-H3 sequence. The CDR-H3 sequence may be selected from CARDDYGDYVAYFQHW, CARDLSYYYGMDVW, CARVYDFWSVLSGFDIW, CARVEQGYDIYYYYYMDVW, CARSYDYGDYLNFDYW, CARASGSGYYYYYGMDVW, CAASTWIQPFDYW, CASNGNYYGSGSYYNYW, CARAVYYDFWSGPFDYW, CAKGGIYYGSGSYPSW, CARGLYYMDVW, CARGLYGDYFLYYGMDVW, CARGLLGFGEFLTYGMDVW, CARDRDSSWTYYYYGMDVW, CARGLYGDYFLYYGMDVW, CARGDYYDSSGYYFPVYFDYW, and CAKDPFWSGHYYYYGMDVW. 
     The ABP specific for A*02:01_AIFPGAVPAA may comprise a CDR-L3 sequence. The CDR-L3 sequence may be selected from CQQNYNSVTF, CQQSYNTPWTF, CGQSYSTPPTF, CQQSYSAPYTF, CQQSYSIPPTF, CQQSYSAPYTF, CQQHNSYPPTF, CQQYSTYPITI, CQQANSFPWTF, CQQSHSTPQTF, CQQSYSTPLTF, CQQSYSTPLTF, CQQTYSTPWTF, CQQYGSSPYTF, CQQSHSTPLTF, CQQANGFPLTF, and CQQSYSTPLTF. 
     The ABP specific for A*02:01_AIFPGAVPAA may comprise a particular heavy chain CDR3 (CDR-H3) sequence and a particular light chain CDR3 (CDR-L3) sequence. In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G8-P1A03, G8-P1A04, G8-P1A06, G8-P1B03, G8-P1C11, G8-P1D02, G8-P1H08, G8-P2B05, G8-P2E06, R3G8-P2C10, R3G8-P2E04, R3G8-P4F05, R3G8-P5C03, R3G8-P5F02, R3G8-P5G08, G8-P1C01, or G8-P2C11. CDR sequences of identified scFvs that specifically bind A*02:01_AIFPGAVPAA are shown in Table 7. For clarity, each identified scFv hit is designated a clone name, and each row contains the CDR sequences for that particular clone name. For example, the scFv identified by clone name G8-P1A03 comprises the heavy chain CDR3 sequence CARDDYGDYVAYFQHW and the light chain CDR3 sequence CQQNYNSVTF. 
     The ABP specific for A*02:01_AIFPGAVPAA may comprise all six CDRs from the scFv designated G8-P1A03, G8-P1A04, G8-P1A06, G8-P1B03, G8-P1C11, G8-P1D02, G8-P1H08, G8-P2B05, G8-P2E06, R3G8-P2C10, R3G8-P2E04, R3G8-P4F05, R3G8-P5C03, R3G8-P5F02, R3G8-P5G08, G8-P1C01, or G8-P2C11. 
     VH 
     The ABP specific for A*02:01_AIFPGAVPAA may comprise a VH sequence. The VH sequence may be selected from 
     
       
         
           
               
            
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSRSAITWVRQAPGQGLEWMG 
               
               
                 WINPNSGATNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DDYGDYVAYFQHWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYPFIGQYLHWVRQAPGQGLEWMG 
               
               
                 IINPSGDSATYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DLSYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYMHWVRQAPGQGLEWMG 
               
               
                 WMNPIGGGTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 VYDFWSVLSGFDIWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFSDYYMSWVRQAPGKGLEWVS 
               
               
                 GINWNGGSTGYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAR 
               
               
                 VEQGYDIYYYYYMDVWGKGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTLSSYPINWVRQAPGQGLEWMG 
               
               
                 WISTYSGHADYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 SYDYGDYLNFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVS 
               
               
                 SISGRGDNTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAR 
               
               
                 ASGSGYYYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFGNYFMHWVRQAPGQGLEWMG 
               
               
                 MVNPSGGSETFAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAA 
               
               
                 STWIQPFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFDFSIYSMNWVRQAPGKGLEWVS 
               
               
                 AISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAS 
               
               
                 NGNYYGSGSYYNYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTLTTYYMHWVRQAPGQGLEWMG 
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 AVYYDFWSGPFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMG 
               
               
                 WINPYSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAK 
               
               
                 GGIYYGSGSYPSWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYGVSWVRQAPGQGLEWMG 
               
               
                 WISPYSGNTDYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAR 
               
               
                 GLYYMDVWGKGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSNMYLHWVRQAPGQGLEWMG 
               
               
                 WINPNTGDTNYAQTFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 GLYGDYFLYYGMDVWGQGTKVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMG 
               
               
                 WMNPNSGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 GLLGFGEFLTYGMDVWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYTHWVRQAPGQGLEWMG 
               
               
                 VINPSGGSTTYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DRDSSWTYYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSNYMHWVRQAPGQGLEWMG 
               
               
                 WMNPNSGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 GLYGDYFLYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSHAISWVRQAPGQGLEWMG 
               
               
                 VIIPSGGTSYTQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARG 
               
               
                 DYYDSSGYYFPVYFDYWGQGTLVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYAMNWVRQAPGQGLEWMG 
               
               
                 WINPNSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DPFWSGHYYYYGMDVWGQGTTVTVSS. 
               
            
           
         
       
     
     VL 
     The ABP specific for A*02:01_AIFPGAVPAA may comprise a VL sequence. The VL sequence may be selected from 
     
       
         
           
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSITSYLNWYQQKPGKAPKLLIY 
               
               
                 DASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQNYNSVTFG 
               
               
                 QGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCWASQGISSYLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYNTPWTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQAISNSLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCGQSYSTPPTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIY 
               
               
                 KASSLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSIPPTF 
               
               
                 GGGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTF 
               
               
                 GGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGINSYLAWYQQKPGKAPKLLIY 
               
               
                 DASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHNSYPPTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYSTYPITI 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNSLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPWTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQDVSTWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSHSTPQTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIY 
               
               
                 DASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTF 
               
               
                 GGGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTF 
               
               
                 GGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNWLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQTYSTPWTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVGNSLAWYQQKPGQAPRLLIY 
               
               
                 GASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYGSSPYTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISGYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSHSTPLTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQNIYTYLNWYQQKPGKAPKLLIY 
               
               
                 DASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANGFPLTF 
               
               
                 GGGTKVEIK, 
               
               
                 and 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTF 
               
               
                 GGGTKVEIK. 
               
            
           
         
       
     
     VH-VL Combinations 
     The ABP specific for A*02:01_AIFPGAVPAA may comprise a particular VH sequence and a particular VL sequence. In some embodiments, the ABP specific for A*02:01_AIFPGAVPAA comprises a VH sequence and VL sequence from the scFv designated G8-P1A03, G8-P1A04, G8-P1A06, G8-P1B03, G8-P1C11, G8-P1D02, G8-P1H08, G8-P2B05, G8-P2E06, R3G8-P2C10, R3G8-P2E04, R3G8-P4F05, R3G8-P5C03, R3G8-P5F02, R3G8-P5G08, G8-P1C01, or G8-P2C11. The VH and VL sequences of identified scFvs that specifically bind A*02:01_AIFPGAVPAA are shown in Table 6. For clarity, each identified scFv hit is designated a clone name, and each row contains the VH and VL sequences for that particular clone name. For example, the scFv identified by clone name G8-P1A03 comprises the VH sequence QVQLVQSGAEVKKPGASVKVSCKASGGTFSRSAITWVRQAPGQGLEWMGWINPNS GATNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDDYGDYVAYFQH WGQGTLVTVSS and the VL sequence 
     
       
         
           
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSITSYLNWYQQKPGKAPKWYDAS 
               
               
                   
               
               
                 NLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQNYNSVTFGQGTK 
               
               
                   
               
               
                 LEIK. 
               
            
           
         
       
     
     Antibodies Specific for A*01:01 ASSLPTTMNY (HLA-PEPTIDE Target “G10”) 
     In some aspects, provided herein are ABPs comprising antibodies or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*01:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises, consists of, or essentially consists of the sequence ASSLPTTMNY (“G10”). 
     CDRs 
     The ABP specific for A*01:01_ASSLPTTMNY may comprise one or more antibody complementarity determining region (CDR) sequences, e.g., may comprise three heavy chain CDRs (CDR-H1, CDR-H2, CDR-H3) and three light chain CDRs (CDR-L1, CDR-L2, CDR-L3). 
     The ABP specific for A*01:01_ASSLPTTMNY may comprise a CDR-H3 sequence. The CDR-H3 sequence may be selected from CARDQDTIFGVVITWFDPW, CARDKVYGDGFDPW, CAREDDSMDVW, CARDSSGLDPW, CARGVGNLDYW, CARDAHQYYDFWSGYYSGTYYYGMDVW, CAREQWPSYWYFDLW, CARDRGYSYGYFDYW, CARGSGDPNYYYYYGLDVW, CARDTGDHFDYW, CARAENGMDVW, CARDPGGYMDVW, CARDGDAFDIW, CARDMGDAFDIW, CAREEDGMDVW, CARDTGDHFDYW, CARGEYSSGFFFVGWFDLW, and CARETGDDAFDIW. 
     The ABP specific for A*01:01_ASSLPTTMNY may comprise a CDR-L3 sequence. The CDR-L3 sequence may be selected from CQQYFTTPYTF, CQQAEAFPYTF, CQQSYSTPITF, CQQSYIIPYTF, CHQTYSTPLTF, CQQAYSFPWTF, CQQGYSTPLTF, CQQANSFPRTF, CQQANSLPYTF, CQQSYSTPFTF, CQQSYSTPFTF, CQQSYGVPTF, CQQSYSTPLTF, CQQSYSTPLTF, CQQYYSYPWTF, CQQSYSTPFTF, CMQTLKTPLSF, and CQQSYSTPLTF. 
     The ABP specific for A*01:01_ASSLPTTMNY may comprise a particular heavy chain CDR3 (CDR-H3) sequence and a particular light chain CDR3 (CDR-L3) sequence. In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated R3G10-P1A07, R3G10-P1B07, R3G10-P1E12, R3G10-P1F06, R3G10-P1H01, R3G10-P1H08, R3G10-P2C04, R3G10-P2G11, R3G10-P3E04, R3G10-P4A02, R3G10-P4C05, R3G10-P4D04, R3G10-P4D10, R3G10-P4E07, R3G10-P4E12, R3G10-P4G06, R3G10-P5A08, or R3G10-P5C08. CDR sequences of identified scFvs that specifically bind A*01:01_ASSLPTTMNY are shown in Table 9. For clarity, each identified scFv hit is designated a clone name, and each row contains the CDR sequences for that particular clone name. For example, the scFv identified by clone name R3G10-P1A07 comprises the heavy chain CDR3 sequence CARDQDTIFGVVITWFDPW and the light chain CDR3 sequence CQQYFTTPYTF. 
     The ABP specific for A*01:01_ASSLPTTMNY may comprise all six CDRs from the scFv designated R3G10-P1A07, R3G10-P1B07, R3G10-P1E12, R3G10-P1F06, R3G10-P1H01, R3G10-P1H08, R3G10-P2C04, R3G10-P2G11, R3G10-P3E04, R3G10-P4A02, R3G10-P4C05, R3G10-P4D04, R3G10-P4D10, R3G10-P4E07, R3G10-P4E12, R3G10-P4G06, R3G10-P5A08, or R3G10-P5C08. 
     VH 
     The ABP specific for A*01:01_ASSLPTTMNY may comprise a VH sequence. The VH sequence may be selected from 
     
       
         
           
               
            
               
                 EVQLLESGGGLVKPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVS 
               
               
                 GISARSGRTYYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCAR 
               
               
                 DQDTIFGVVITWFDPWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMG 
               
               
                 IIHPGGGTTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DKVYGDGFDPWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYIFTGYYMHWVRQAPGQGLEWMG 
               
               
                 MIGPSDGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 EDDSMDVWGKGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFIGYYMHWVRQAPGQGLEWMG 
               
               
                 MIGPSDGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DSSGLDPWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMG 
               
               
                 MIGPSDGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 GVGNLDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGVTFSTSAISWVRQAPGQGLEWMG 
               
               
                 WISPYNGNTDYAQMLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DAHQYYDFWSGYYSGTYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSNSIINWVRQAPGQGLEWMG 
               
               
                 WMNPNSGNTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 EQWPSYWYFDLWGRGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSTHDINWVRQAPGQGLEWMG 
               
               
                 VINPSGGSAIYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DRGYSYGYFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGNTFIGYYVHWVRQAPGQGLEWVG 
               
               
                 IINPNGGSISYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 GSGDPNYYYYYGLDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTLSYYYMHWVRQAPGQGLEWMG 
               
               
                 MIGPSDGSTSYAQRFQGRVTMTRDTSTGTVYMELSSLRSEDTAVYYCAR 
               
               
                 DTGDHFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMG 
               
               
                 IIGPSDGSTTYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 AENGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYVHWVRQAPGQGLEWMG 
               
               
                 IIAPSDGSTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DPGGYMDVWGKGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYLHWVRQAPGQGLEWMG 
               
               
                 MIGPSDGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DGDAFDIWGQGTMVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMG 
               
               
                 RISPSDGSTTYAPKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAR 
               
               
                 DMGDAFDIWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMG 
               
               
                 MIGPSDGSTSYAQRFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 EEDGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTLSYYYMHWVRQAPGQGLEWMG 
               
               
                 MIGPSDGSTSYAQRFQGRVTMTRDTSTGTVYMELSSLRSEDTAVYYCAR 
               
               
                 DTGDHFDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGGTFNNFAISWVRQAPGQGLEWMG 
               
               
                 GIIPIFDATNYAQKFQGRVTFTADESTSTAYMELSSLRSEDTAVYYCAR 
               
               
                 GEYSSGFFFVGWFDLWGRGTQVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYNFTGYYMHWVRQAPGQGLEWMG 
               
               
                 IIAPSDGSTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 ETGDDAFDIWGQGTMVTVSS. 
               
            
           
         
       
     
     VL 
     The ABP specific for A*01:01_ASSLPTTMNY may comprise a VL sequence. The VL sequence may be selected from 
     
       
         
           
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQGGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYFTTPYTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIF 
               
               
                 DASRLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQAEAFPYTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPITF 
               
               
                 GQGTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISNYLNWYQQKPGKAPKLLIY 
               
               
                 KASSLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYIIPYTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISNYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQTYSTPLTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIY 
               
               
                 SASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQAYSFPWTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQNISSYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGYSTPLTF 
               
               
                 GQGTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQDISRYLAWYQQKPGKAPKLLIY 
               
               
                 DASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPRTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 AASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSLPYTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIY 
               
               
                 AASTLQNGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQRISSYLNWYQQKPGKAPKLLIY 
               
               
                 SASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLAWYQQKPGKAPKLLIY 
               
               
                 DASKLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYGVPTFG 
               
               
                 QGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIY 
               
               
                 DASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTF 
               
               
                 GGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTF 
               
               
                 GGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISTYLAWYQQKPGKAPKLLIY 
               
               
                 DASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYSYPWTF 
               
               
                 GQGTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGSISSYLNWYQQKPGKAPKLLI 
               
               
                 YAASTLQNGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFT 
               
               
                 FGPGTKVDIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSP 
               
               
                 QLLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQTLK 
               
               
                 TPLSFGGGTKVEIK, 
               
               
                 and 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTF 
               
               
                 GGGTKVEIK. 
               
            
           
         
       
     
     VH-VL Combinations 
     The ABP specific for A*01:01_ASSLPTTMNY may comprise a particular VH sequence and a particular VL sequence. In some embodiments, the ABP specific for A*01:01_ASSLPTTMNY comprises a VH sequence and VL sequence from the scFv designated R3G10-P1A07, R3G10-P1B07, R3G10-P1E12, R3G10-P1F06, R3G10-P1H01, R3G10-P1H08, R3G10-P2C04, R3G10-P2G11, R3G10-P3E04, R3G10-P4A02, R3G10-P4C05, R3G10-P4D04, R3G10-P4D10, R3G10-P4E07, R3G10-P4E12, R3G10-P4G06, R3G10-P5A08, or R3G10-P5C08. The VH and VL sequences of identified scFvs that specifically bind A*01:01_ASSLPTTMNY are shown in Table 8. For clarity, each identified scFv hit is designated a clone name, and each row contains the VH and VL sequences for that particular clone name. For example, the scFv identified by clone name R3G10-P1A07 comprises the VH sequence EVQLLESGGGLVKPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVSGISARSG RTYYADSVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCARDQDTIFGVVITWFDP WGQGTLVTVSS and the VL sequence 
     
       
         
           
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLQGGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYFTTPYTFGQ 
               
               
                   
               
               
                 GTKLEIK. 
               
            
           
         
       
     
     Antibodies Specific for A*02:01 LLASSILCA (G7) 
     In some aspects, provided herein are ABPs comprising antibodies or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*02:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises, consists of, or consists essentially of the sequence LLASSILCA (“G7”). 
     Sequences of G7-Specific Antibodies 
     The ABP specific for A*02:01_LLASSILCA may comprise one or more sequences, as described in further detail. 
     CDRs 
     The ABP specific for A*02:01_LLASSILCA may comprise one or more antibody complementarity determining region (CDR) sequences, e.g., may comprise three heavy chain CDRs (CDR-H1, CDR-H2, CDR-H3) and three light chain CDRs (CDR-L1, CDR-L2, CDR-L3). 
     The ABP specific for A*02:01_LLASSILCA may comprise a CDR-H3 sequence. The CDR-H3 sequence may be selected from CARDGYDFWSGYTSDDYW, CASDYGDYR, CARDLMTTVVTPGDYGMDVW, CARQDGGAFAFDIW, CARELGYYYGMDVW, CARALIFGVPLLPYGMDVW, CAKDLATVGEPYYYYGMDVW, and CARLWFGELHYYYYYGMDVW. 
     The ABP specific for A*02:01_LLASSILCA may comprise a CDR-L3 sequence. The CDR-L3 sequence may be selected from CHHYGRSHTF, CQQANAFPPTF, CQQYYSIPLTF, CQQSYSTPPTF, CQQSYSFPYTF, CMQALQTPLTF, CQQGNTFPLTF, and CMQGSHWPPSF. 
     The ABP specific for A*02:01_LLASSILCA may comprise a particular heavy chain CDR3 (CDR-H3) sequence and a particular light chain CDR3 (CDR-L3) sequence. In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G7R3-P1C6, G7R3-P1G10, 1-G7R3-P1B4, 2-G7R4-P2C2, 3-G7R4-P1A3, 4-G7R4-B5-P2E9, 5-G7R4-B10-P1F8, or B7 (G7R3-P3A9). CDR sequences of identified scFvs that specifically bind A*02:01_LLASSILCA are shown in Table 36. For clarity, each identified scFv hit is designated a clone name, and each row contains the CDR sequences for that particular clone name. For example, the scFv identified by clone name G7R3-P1C6 comprises the heavy chain CDR3 sequence CARDGYDFWSGYTSDDYW and the light chain CDR3 sequence CHHYGRSHTF. 
     The ABP specific for A*02:01_LLASSILCA may comprise all six CDRs from the scFv designated G7R3-P1C6, G7R3-P1G10, 1-G7R3-P1B4, 2-G7R4-P2C2, 3-G7R4-P1A3, 4-G7R4-B5-P2E9, 5-G7R4-B10-P1F8, or B7 (G7R3-P3A9). 
     VL 
     The ABP specific for *02:01_LLASSILCA may comprise a VL sequence. The VL sequence may be selected from 
     
       
         
           
               
            
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVSSSNLAWYQQKPGQAPRLLI 
               
               
                 YGASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCHHYGRSHTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQDIRNDLGWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANAFPPTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIVMTQSPDSLAVSLGERATINCKSSQSVFYSSNNKNQLAWYQQKPGQP 
               
               
                 PKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYY 
               
               
                 SIPLTFGQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDIFKYLNWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPPTF 
               
               
                 GQGTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISTWLAWYQQKPGKAPKLLIY 
               
               
                 YASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSFPYTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCSSSQSLLHSNGYNYLDWYLQKPGQSP 
               
               
                 QLLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQALQ 
               
               
                 TPLTFGGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 SASNLRSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQGNTFPLTF 
               
               
                 GQGTKVEIK, 
               
               
                 and 
               
               
                   
               
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSP 
               
               
                 QLLIYLGSNRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGSH 
               
               
                 WPPSFGQGTRLEIK. 
               
            
           
         
       
     
     VH 
     The ABP specific for *02:01_LLASSILCA may comprise a VH sequence. The VH sequence may be selected from 
     
       
         
           
               
            
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSNYGISWVRQAPGQGLEWMG 
               
               
                 IINPGGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARD 
               
               
                 GYDFWSGYTSDDYWGQGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMHWVRQAPGKGLEWVS 
               
               
                 GISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAS 
               
               
                 DYGDYRGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSNYYIHWVRQAPGQGLEWMG 
               
               
                 WLNPNSGNTGYAQRFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 DLMTTVVTPGDYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASMKVSCKASGYTFTTDGISWVRQAPGQGLEWMG 
               
               
                 RIYPHSGYTEYAKKFKGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 QDGGAFAFDIWGQGTMVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSQYMHWVRQAPGQGLEWMG 
               
               
                 WISPNNGDTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR 
               
               
                 ELGYYYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASRYTFTSYDINWVRQAPGQGLEWMG 
               
               
                 RIIPMLNIANYAPKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAR 
               
               
                 ALIFGVPLLPYGMDVWGQGTTVTVSS, 
               
               
                   
               
               
                 EVQLLQSGGGLVQPGGSLRLSCAASGFTFSSSWMHWVRQAPGKGLEWVS 
               
               
                 FISTSSGYIYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAK 
               
               
                 DLATVGEPYYYYGMDVWGQGTTVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGDTFNTYALSWVRQAPGQGLEWMG 
               
               
                 WMNPNSGNAGYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAR 
               
               
                 LWFGELHYYYYYGMDVWGQGTMVTVSS. 
               
            
           
         
       
     
     VH-VL Combinations 
     The ABP specific for A*02:01_LLASSILCA may comprise a particular VH sequence and a particular VL sequence. In some embodiments, the ABP specific for A*02:01_LLASSILCA comprises a VH sequence and a VL sequence from the scFv designated G7R3-P1C6, G7R3-P1G10, 1-G7R3-P1B4, 2-G7R4-P2C2, 3-G7R4-P1A3, 4-G7R4-B5-P2E9, 5-G7R4-B10-P1F8, or B7 (G7R3-P3A9). The VH and VL sequences of identified scFvs that specifically bind A*02:01_LLASSILCA are shown in Table 35. For clarity, each identified scFv hit is designated a clone name, and each row contains the VH and VL sequences for that particular clone name. For example, the scFv identified by clone name G7R3-P1C6 comprises the VH sequence QVQLVQSGAEVKKPGASVKVSCKASGGTFSNYGISWVRQAPGQGLEWMGIINPGGS TSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDGYDFWSGYTSDDY WGQGTLVTVSS and the VL sequence 
     
       
         
           
               
            
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVSSSNLAWYQQKPGQAPRLLIY 
               
               
                   
               
               
                 GASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCHHYGRSHTFGQ 
               
               
                   
               
               
                 GTKVEIK. 
               
            
           
         
       
     
     Antibodies Specific for A*01:01 NTDNNLAVY (G2) 
     In some aspects, provided herein are ABPs comprising antibodies or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*01:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises, consists of, or consists essentially of the sequence NTDNNLAVY (“G2”). 
     Sequences of G2-Specific Antibodies 
     The ABP specific for A*01:01_NTDNNLAVY may comprise one or more sequences, as described in further detail. 
     CDRs 
     The ABP specific for A*01:01_NTDNNLAVY may comprise one or more antibody complementarity determining region (CDR) sequences, e.g., may comprise three heavy chain CDRs (CDR-H1, CDR-H2, CDR-H3) and three light chain CDRs (CDR-L1, CDR-L2, CDR-L3). 
     The ABP specific for A*01:01_NTDNNLAVY may comprise a CDR-H3 sequence. The CDR-H3 sequence may be selected from CAATEWLGVW, CARANWLDYW, CARANWLDYW, CARDWVLDYW, CARGEWLDYW, CARGWELGYW, CARDFVGYDDW, CARDYGDLDYW, CARGSYGMDVW, CARDGYSGLDVW, CARDSGVGMDVW, CARDGVAVASDYW, CARGVNVDDFDYW, CARGDYTGNWYFDLW, CARANWLDYW, CARDQFYGGNSGGHDYW, CAREEDYW, CARGDWFDPW, CARGDWFDPW, CARGEWFDPW, CARSDWFDPW, CARDSGSYFDYW, CARDYGGYVDYW, CAREGPAALDVW, CARERRSGMDVW, CARVLQEGMDVW, CASERELPFDIW, CAKGGGGYGMDVW, CAAMGIAVAGGMDVW, CARNWNLDYW, CATYDDGMDVW, CARGGGGALDYW, CALSGNYYGMDVW, CARGNPWELRLDYW, and CARDKNYYGMDVW. 
     The ABP specific for A*01:01_NTDNNLAVY may comprise a CDR-L3 sequence. The CDR-L3 sequence may be selected from CQQSYNTPYTF, CQQSYSTPYTF, CQQSYSTPYSF, CQQSYSTPFTF, CQQSYGVPYTF, CQQSYSAPYTF, CQQSYSAPYTF, CQQSYSAPYSF, CQQSYSTPYTF, CQQSYSVPYSF, CQQSYSAPYTF, CQQSYSVPYSF, CQQSYSTPQTF, CQQLDSYPFTF, CQQSYSSPYTF, CQQSYSTPLTF, CQQSYSTPYSF, CQQSYSTPYTF, CQQSYSTPYTF, CQQSYSTPFTF, CQQSYSTPTF, CQQTYAIPLTF, CQQSYSTPYTF, CQQSYIAPFTF, CQQSYSIPLTF, CQQSYSNPTF, CQQSYSTPYSF, CQQSYSDQWTF, CQQSYLPPYSF, CQQSYSSPYTF, CQQSYTTPWTF, CQQSYLPPYSF, CQEGITYTF, CQQYYSYPFTF, and CQHYGYSPVTF. 
     The ABP specific for A*01:01_NTDNNLAVY may comprise a particular heavy chain CDR3 (CDR-H3) sequence and a particular light chain CDR3 (CDR-L3) sequence. In some embodiments, the ABP comprises the CDR-H3 and the CDR-L3 from the scFv designated G2-P2E07, G2-P2E03, G2-P2A11, G2-P2C06, G2-P1G01, G2-P1C02, G2-P1H01, G2-P1B12, G2-P1B06, G2-P2H10, G2-P1H10, G2-P2C11, G2-P1C09, G2-P1A10, G2-P1B10, G2-P1D07, G2-P1E05, G2-P1D03, G2-P1G12, G2-P2H11, G2-P1C03, G2-P1G07, G2-P1F12, G2-P1G03, G2-P2B08, G2-P2A10, G2-P2D04, G2-P1C06, G2-P2A09, G2-P1B08, G2-P1E03, G2-P2A03, G2-P2F01, G2-P1H11, or G2-P1D06. CDR sequences of identified scFvs that specifically bind A*01:01_NTDNNLAVY are found in Table 34. For clarity, each identified scFv hit is designated a clone name, and each row contains the CDR sequences for that particular clone name. For example, the scFv identified by clone name G2-P2E07 comprises the heavy chain CDR3 sequence CAATEWLGVW and the light chain CDR3 sequence CQQSYNTPYTF. 
     The ABP specific for A*01:01_NTDNNLAVY may comprise all six CDRs from the scFv designated G2-P2E07, G2-P2E03, G2-P2A11, G2-P2C06, G2-P1G01, G2-P1C02, G2-P1H01, G2-P1B12, G2-P1B06, G2-P2H10, G2-P1H10, G2-P2C11, G2-P1C09, G2-P1A10, G2-P1B10, G2-P1D07, G2-P1E05, G2-P1D03, G2-P1G12, G2-P2H11, G2-P1C03, G2-P1G07, G2-P1F12, G2-P1G03, G2-P2B08, G2-P2A10, G2-P2D04, G2-P1C06, G2-P2A09, G2-P1B08, G2-P1E03, G2-P2A03, G2-P2F01, G2-P1H11, or G2-P1D06. 
     VL 
     The ABP specific for A*01:01_NTDNNLAVY may comprise a VL sequence. The VL sequence may be selected from 
     
       
         
           
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISTWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLRSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYNTPYTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIY 
               
               
                 AASTVQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQDISRWLAWYQQKPGKAPKLLIY 
               
               
                 AASRLQAGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYSF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQTISSWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQTISSWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYGVPYTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISNWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSVGNWLAWYQQKPGKAPKLLIY 
               
               
                 GASSLQTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQNIGNWLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYSF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIY 
               
               
                 GASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSVPYSF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISKWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSAPYTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSVPYSF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQTISNYLNWYQQKPGKAPKLLIY 
               
               
                 AASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPQTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASRDIGRAVGWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQLDSYPFTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSSPYTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPLTF 
               
               
                 GGGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSIGRWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYSF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISTWLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTF 
               
               
                 AQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 GASRLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPFTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSVSNWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPTFG 
               
               
                 QGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQTYAIPLTF 
               
               
                 GGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDIGSWLAWYQQKPGKAPKLLIY 
               
               
                 ATSSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISRWLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQPGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYIAPFTF 
               
               
                 GPGTKVDIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIY 
               
               
                 AASRLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSIPLTF 
               
               
                 GGGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIY 
               
               
                 GVSSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSNPTFG 
               
               
                 QGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWVAWYQQKPGKAPKLLIY 
               
               
                 GASNLESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTPYSF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSDQWTF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISRWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYLPPYSF 
               
               
                 GQGTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISNWLAWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTYFTLTISSLQPEDFATYYCQQSYSSPYTF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISHYLNWYQQKPGKAPKLLIY 
               
               
                 GASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYTTPWTF 
               
               
                 GQGTRLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIY 
               
               
                 AASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYLPPYSF 
               
               
                 GQGTKLEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 GASRLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQEGITYTFGQ 
               
               
                 GTKVEIK, 
               
               
                   
               
               
                 DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIY 
               
               
                 AASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYSYPFTF 
               
               
                 GPGTKVDIK, 
               
               
                 and 
               
               
                   
               
               
                 EIVMTQSPATLSVSPGERATLSCRASQSVSRNLAWYQQKPGQAPRLLIY 
               
               
                 GASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQHYGYSPVTF 
               
               
                 GQGTKLEIK. 
               
            
           
         
       
     
     VH 
     The ABP specific for A*01:01_NTDNNLAVY may comprise a VH sequence. The VH sequence may be selected from 
     
       
         
           
               
               
            
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSATISWVRQAPGQGLEWMGWIYPNS 
                   
               
               
                 GGTVYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAATEWLGVWGQGTT 
               
               
                 VTVSS, 
               
               
                   
               
               
                 EVQLLQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGWINPNSG 
               
               
                 GTISAPNFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARANWLDYWGQGTLVT 
               
               
                 VSS, 
               
               
                   
               
               
                 EVQLLESGAEVKKPGASVKVSCKASGYTFTTYDLAWVRQAPGQGLEWMGWINPNS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARANWLDYWGQGT 
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKSSGYSFDSYVVNWVRQAPGQGLEWMGWINPN 
               
               
                 SGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDWVLDYWGQG 
               
               
                 TLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMGWMNPN 
               
               
                 SGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGEWLDYWGQGT 
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMGWINPNS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGWELGYWGQGTL 
               
               
                 VTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTRYTINWVRQAPGQGLEWMGWINPNS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDFVGYDDWGQGT 
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGITWVRQAPGQGLEWMGWINPNS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDYGDLDYWGQGT 
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSNYILSWVRQAPGQGLEWMGWINPDS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGSYGMDVWGQG 
               
               
                 TTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYSFTRYNMHWVRQAPGQGLEWMGWINPN 
               
               
                 SGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDGYSGLDVWGK 
               
               
                 GTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGWINPNN 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDSGVGMDVWGQ 
               
               
                 GTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFNNYAFSWVRQAPGQGLEWMGWINPN 
               
               
                 SGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDGVAVASDYWG 
               
               
                 QGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSSYNMHWVRQAPGQGLEWMGWINGN 
               
               
                 TGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGVNVDDFDYWG 
               
               
                 QGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYAFSWVRQAPGQGLEWMGWINPDT 
               
               
                 GYTRYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGDYTGNWYFDLW 
               
               
                 GRGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMGWINPYSG 
               
               
                 GTNYAQKLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARANWLDYWGQGTL 
               
               
                 VTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMGWISAYN 
               
               
                 GYTNYAQNLQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDQFYGGNSGGHD 
               
               
                 YWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYNMHWVRQAPGQGLEWMGWMNP 
               
               
                 NSGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARE- 
               
               
                 EDYWGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTRYTINWVRQAPGQGLEWMGWINPNS 
               
               
                 GGANYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGDWFDPWGQGTL 
               
               
                 VTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYLMHWVRQAPGQGLEWMGWISPN 
               
               
                 SGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGDWFDPWGQGT 
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSDYYVHWVRQAPGQGLEWMGWINPN 
               
               
                 SGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGEWFDPWGQGT 
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTTYYMHWVRQAPGQGLEWMGWINPN 
               
               
                 SGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARSDWFDPWGQGT 
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSNYAINWVRQAPGQGLEWMGWISPYS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDSGSYFDYWGQG 
               
               
                 TLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYYMHWVRQAPGQGLEWMGWIYPN 
               
               
                 TGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDYGGYVDYWG 
               
               
                 QGTLVTVSS, 
               
               
                   
               
               
                 EVQLLESGAEVKKPGASVKVSCKASGYTFTSYAMNWVRQAPGQGLEWMGWMNPN 
               
               
                 SGGTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAREGPAALDVWGQ 
               
               
                 GTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTLTSHLIHWVRQAPGQGLEWMGWINPNS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARERRSGMDVWGQG 
               
               
                 TTVTVSS, 
               
               
                   
               
               
                 EVQLLESGAEVKKPGASVKVSCKASGYSFTDYIVHWVRQAPGQGLEWMGWINPYS 
               
               
                 GGTKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARVLQEGMDVWGQ 
               
               
                 GTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFSNFLINWVRQAPGQGLEWMGWINPNS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCASERELPFDIWGQGT 
               
               
                 MVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYQMFWVRQAPGQGLEWMGWINPN 
               
               
                 SGGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAKGGGGYGMDVW 
               
               
                 GQGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGWINPNS 
               
               
                 GGTNYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAAMGIAVAGGMDV 
               
               
                 WGQGTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYHMHWVRQAPGQGLEWMGWIHPD 
               
               
                 SGGTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARNWNLDYWGQGT 
               
               
                 LVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGWMNP 
               
               
                 NSGNTGYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCATYDDGMDVWG 
               
               
                 QGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYTVNWVRQAPGQGLEWMGWINPN 
               
               
                 SGGTKYAQNFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGGGGALDYWGQ 
               
               
                 GTLVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGMINPR 
               
               
                 DDTTDYARDFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCALSGNYYGMDVWG 
               
               
                 QGTTVTVSS, 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYYMHWVRQAPGQGLEWMGMINPS 
               
               
                 GGGTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGNPWELRLDYW 
               
               
                 GQGTLVTVSS, 
               
               
                 and 
               
               
                   
               
               
                 QVQLVQSGAEVKKPGSSVKVSCKASGYTFTSQYMHWVRQAPGQGLEWMGRIIPLL 
               
               
                 GIVNYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARDKNYYGMDVWGQ 
               
               
                 GTTVTVSS. 
               
            
           
         
       
     
     VH-VL Combinations 
     The ABP specific for A*01:01_NTDNNLAVY may comprise a particular VH sequence and a particular VL sequence. In some embodiments, the ABP specific for A*01:01_NTDNNLAVY comprises the VH sequence and the VL sequence from the scFv designated G2-P2E07, G2-P2E03, G2-P2A11, G2-P2C06, G2-P1G01, G2-P1C02, G2-P1H01, G2-P1B12, G2-P1B06, G2-P2H10, G2-P1H10, G2-P2C11, G2-P1C09, G2-P1A10, G2-P1B10, G2-P1D07, G2-P1E05, G2-P1D03, G2-P1G12, G2-P2H11, G2-P1C03, G2-P1G07, G2-P1F12, G2-P1G03, G2-P2B08, G2-P2A10, G2-P2D04, G2-P1C06, G2-P2A09, G2-P1B08, G2-P1E03, G2-P2A03, G2-P2F01, G2-P1H11, or G2-P1D06. VH and VL sequences of identified scFvs that specifically bind A*01:01_NTDNNLAVY are found in Table 33. For clarity, each identified scFv hit is designated a clone name, and each row contains the CDR sequences for that particular clone name. For example, the scFv identified by clone name G2-P2E07 comprises the VH sequence QVQLVQSGAEVKKPGASVKVSCKASGGTFSSATISWVRQAPGQGLEWMGWIYPNS GGTVYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAATEWLGVWGQGTT VTVSSAS and the VL sequence 
     
       
         
           
               
            
               
                 DIQMTQSPSSLSASVGDRVTITCRASQSISTWLAWYQQKPGKAPKLLIYA 
               
               
                   
               
               
                 ASSLRSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSYNTPYTFGQ 
               
               
                   
               
               
                 GTKLEIK. 
               
            
           
         
       
     
     Receptors 
     Among the provided ABPs, e.g., HLA-PEPTIDE ABPs, are receptors. The receptors can include antigen receptors and other chimeric receptors that specifically bind an HLA-PEPTIDE target disclosed herein. The receptor may be a T cell receptor (TCR). The receptor may be a chimeric antigen receptor (CAR). 
     TCRs can be soluble or membrane-bound. Among the antigen receptors are functional non-TCR antigen receptors, such as chimeric antigen receptors (CARs). Also provided are cells expressing the receptors and uses thereof in adoptive cell therapy, such as treatment of diseases and disorders associated with HLA-PEPTIDE expression, including cancer. 
     Exemplary antigen receptors, including CARs, and methods for engineering and introducing such receptors into cells, include those described, for example, in international patent application publication numbers WO200014257, WO2013126726, WO2012/129514, WO2014031687, WO2013/166321, WO2013/071154, WO2013/123061 U.S. patent application publication numbers US2002131960, US2013287748, US20130149337, U.S. Pat. Nos. 6,451,995, 7,446,190, 8,252,592, 8,339,645, 8,398,282, 7,446,179, 6,410,319, 7,070,995, 7,265,209, 7,354,762, 7,446,191, 8,324,353, and 8,479,118, and European patent application number EP2537416, and/or those described by Sadelain et al., Cancer Discov. 2013 April; 3(4): 388-398; Davila et al. (2013) PLoS ONE 8(4): e61338; Turtle et al., Curr. Opin. Immunol., 2012 October; 24(5): 633-39; Wu et al., Cancer, 2012 Mar. 18(2): 160-75. In some aspects, the antigen receptors include a CAR as described in U.S. Pat. No. 7,446,190, and those described in International Patent Application Publication No.: WO/2014055668 A1. Exemplary of the CARs include CARs as disclosed in any of the aforementioned publications, such as WO2014031687, U.S. Pat. Nos. 8,339,645, 7,446,179, US 2013/0149337, U.S. Pat. Nos. 7,446,190, 8,389,282, e.g., and in which the antigen-binding portion, e.g., scFv, is replaced by an antibody, e.g., as provided herein. 
     Among the chimeric receptors are chimeric antigen receptors (CARs). The chimeric receptors, such as CARs, generally include an extracellular antigen binding domain that includes, is, or is comprised within, one of the provided anti-HLA-PEPTIDE ABPs such as anti-HLA-PEPTIDE antibodies. Thus, the chimeric receptors, e.g., CARs, typically include in their extracellular portions one or more HLA-PEPTIDE-ABPs, such as one or more antigen-binding fragment, domain, or portion, or one or more antibody variable domains, and/or antibody molecules, such as those described herein. In some embodiments, the CAR includes a HLA-PEPTIDE-binding portion or portions of the ABP (e.g., antibody) molecule, such as a variable heavy (VH) chain region and/or variable light (VL) chain region of the antibody, e.g., an scFv antibody fragment. 
     TCRs 
     In an aspect, the ABPs provided herein, e.g., ABPs that specifically bind HLA-PEPTIDE targets disclosed herein, include T cell receptors (TCRs). The TCRs may be isolated and purified. 
     In a majority of T-cells, the TCR is a heterodimer polypeptide having an alpha (α) chain and beta-(β) chain, encoded by TRA and TRB, respectively. The alpha chain generally comprises an alpha variable region, encoded by TRAV, an alpha joining region, encoded by TRAJ, and an alpha constant region, encoded by TRAC. The beta chain generally comprises a beta variable region, encoded by TRBV, a beta diversity region, encoded by TRBD, a beta joining region, encoded by TRBJ, and a beta constant region, encoded by TRBC. The TCR-alpha chain is generated by VJ recombination, and the beta chain receptor is generated by V(D)J recombination. Additional TCR diversity stems from junctional diversity. Several bases may be deleted and others added (called N and P nucleotides) at each of the junctions. In a minority of T-cells, the TCRs include gamma and delta chains. The TCR gamma chain is generated by VJ recombination, and the TCR delta chain is generated by V(D)J recombination (Kenneth Murphy, Paul Travers, and Mark Walport, Janeway&#39;s Immunology 7th edition, Garland Science, 2007, which is herein incorporated by reference in its entirety). The antigen binding site of a TCR generally comprises six complementarity determining regions (CDRs). The alpha chain contributes three CDRs, alpha CDR1, alpha CDR2, and αCDR3. The beta chain also contributes three CDR: beta CDR1, beta CDR2, and βCDR3. The αCDR3 and βCDR3 are the regions most affected by V(D)J recombination and account for most of the variation in a TCR repertoire. 
     TCRs can specifically recognize HLA-PEPTIDE targets, such as an HLA-PEPTIDE target disclosed in Table A, A1, or A2; thus TCRs can be ABPs that specifically bind to HLA-PEPTIDE. TCRs can be soluble, e.g., similar to an antibody secreted by a B cell. TCRs can also be membrane-bound, e.g., on a cell such as a T cell or natural killer (NK) cell. Thus, TCRs can be used in a context that corresponds to soluble antibodies and/or membrane-bound CARs. 
     Any of the TCRs disclosed herein may comprise an alpha variable region, an alpha joining region, optionally an alpha constant region, a beta variable region, optionally a beta diversity region, a beta joining region, and optionally a beta constant region. 
     In some embodiments, the TCR or CAR is a recombinant TCR or CAR. The recombinant TCR or CAR may include any of the TCRs identified herein but include one or more modifications. Exemplary modifications, e.g., amino acid substitutions, are described herein. Amino acid substitutions described herein may be made with reference to IMGT nomenclature and amino acid numbering as found at www.imgt.org. 
     The recombinant TCR or CAR may be a human TCR or CAR, comprising fully human sequences, e.g., natural human sequences. The recombinant TCR or CAR may retain its natural human variable domain sequences but contain modifications to the α constant region, β constant region, or both α and β constant regions. Such modifications to the TCR constant regions may improve TCR assembly and expression for TCR gene therapy by, e.g., driving preferential pairings of the exogenous TCR chains. 
     In some embodiments, the α and β constant regions are modified by substituting the entire human constant region sequences for mouse constant region sequences. Such “murinized” TCRs and methods of making them are described in Cancer Res. 2006 Sep. 1; 66(17):8878-86, which is hereby incorporated by reference in its entirety. 
     In some embodiments, the α and β constant regions are modified by making one or more amino acid substitutions in the human TCR α constant (TRAC) region, the TCR β constant (TRBC) region, or the TRAC and TRAB regions, which swap particular human residues for murine residues (human→murine amino acid exchange). The one or more amino acid substitutions in the TRAC region may include a Ser substitution at residue 90, an Asp substitution at residue 91, a Val substitution at residue 92, a Pro substitution at residue 93, or any combination thereof. The one or more amino acid substitutions in the human TRBC region may include a Lys substitution at residue 18, an Ala substitution at residue 22, an Ile substitution at residue 133, a His substitution at residue 139, or any combination of the above. Such targeted amino acid substitutions are described in J Immunol Jun. 1, 2010, 184 (11) 6223-6231, which is hereby incorporated by reference in its entirety. 
     In some embodiments, the human TRAC contains an Asp substitution at residue 210 and the human TRBC contains a Lys substitution at residue 134. Such substitutions may promote the formation of a salt bridge between the alpha and beta chains and formation of the TCR interchain disulfide bond. These targeted substitutions are described in J Immunol Jun. 1, 2010, 184 (11) 6232-6241, which is hereby incorporated by reference in its entirety. 
     In some embodiments, the human TRAC and human TRBC regions are modified to contain introduced cysteines which may improve preferential pairing of the exogenous TCR chains through formation of an additional disulfide bond. For example, the human TRAC may contain a Cys substitution at residue 48 and the human TRBC may contain a Cys substitution at residue 57, described in Cancer Res. 2007 Apr. 15; 67(8):3898-903 and Blood. 2007 Mar. 15; 109(6):2331-8, which are hereby incorporated by reference in their entirety. 
     The recombinant TCR or CAR may comprise other modifications to the α and β chains. 
     In some embodiments, the α and β chains are modified by linking the extracellular domains of the α and β chains to a complete human CD3ζ (CD3-zeta) molecule. Such modifications are described in J Immunol Jun. 1, 2008, 180 (11) 7736-7746; Gene Ther. 2000 August; 7(16):1369-77; and The Open Gene Therapy Journal, 2011, 4: 11-22, which are hereby incorporated by reference in their entirety. 
     In some embodiments, the α chain is modified by introducing hydrophobic amino acid substitutions in the transmembrane region of the α chain, as described in J Immunol Jun. 1, 2012, 188 (11) 5538-5546; hereby incorporated by reference in their entirety. 
     The alpha or beta chain may be modified by altering any one of the N-glycosylation sites in the amino acid sequence, as described in J Exp Med. 2009 Feb. 16; 206(2): 463-475; hereby incorporated by reference in its entirety. 
     The alpha and beta chain may each comprise a dimerization domain, e.g., a heterologous dimerization domain. Such a heterologous domain may be a leucine zipper, a 5H3 domain or hydrophobic proline rich counter domains, or other similar modalities, as known in the art. In one example, the alpha and beta chains may be modified by introducing 30mer segments to the carboxyl termini of the alpha and beta extracellular domains, wherein the segments selectively associate to form a stable leucine zipper. Such modifications are described in PNAS Nov. 22, 1994. 91 (24) 11408-11412; https://doi.org/10.1073/pnas.91.24.11408; hereby incorporated by reference in its entirety. 
     TCRs identified herein may be modified to include mutations that result in increased affinity or half-life, such as those described in WO2012/013913, hereby incorporated by reference in its entirety. 
     The recombinant TCR or CAR may be a single chain TCR (scTCR). Such scTCR may comprise an α chain variable region sequence fused to the N terminus of a TCR α chain constant region extracellular sequence, a TCR β chain variable region fused to the N terminus of a TCR β chain constant region extracellular sequence, and a linker sequence linking the C terminus of the α segment to the N terminus of the β segment, or vice versa. In some embodiments, the constant region extracellular sequences of the α and β segments of the scTCR are linked by a disulfide bond. In some embodiments, the length of the linker sequence and the position of the disulfide bond being such that the variable region sequences of the α and β segments are mutually orientated substantially as in native αβ T cell receptors. Exemplary scTCRs are described in U.S. Pat. No. 7,569,664, which is hereby incorporated by reference in its entirety. 
     In some cases, the variable regions of the scTCR may be covalently joined by a short peptide linker, such as described in Gene Therapy volume 7, pages 1369-1377 (2000). The short peptide linker may be a serine rich or glycine rich linker. For example, the linker may be (Gly 4 Ser) 3 , as described in Cancer Gene Therapy (2004) 11, 487-496, incorporated by reference in its entirety. 
     The recombinant TCR or antigen binding fragment thereof may be expressed as a fusion protein. For instance, the TCR or antigen binding fragment thereof may be fused with a toxin. Such fusion proteins are described in Cancer Res. 2002 Mar. 15; 62(6):1757-60. The TCR or antigen binding fragment thereof may be fused with an antibody Fc region. Such fusion proteins are described in J Immunol May 1, 2017, 198 (1 Supplement) 120.9. 
     In some embodiments, the recombinant receptor such as a TCR or CAR, such as the antibody portion thereof, further includes a spacer, which may be or include at least a portion of an immunoglobulin constant region or variant or modified version thereof, such as a hinge region, e.g., an IgG4 hinge region, and/or a CH1/CL and/or Fc region. In some embodiments, the constant region or portion is of a human IgG, such as IgG4 or IgG1. In some aspects, the portion of the constant region serves as a spacer region between the antigen-recognition component, e.g., scFv, and transmembrane domain. The spacer can be of a length that provides for increased responsiveness of the cell following antigen binding, as compared to in the absence of the spacer. In some examples, the spacer is at or about 12 amino acids in length or is no more than 12 amino acids in length. Exemplary spacers include those having at least about 10 to 229 amino acids, about 10 to 200 amino acids, about 10 to 175 amino acids, about 10 to 150 amino acids, about 10 to 125 amino acids, about 10 to 100 amino acids, about 10 to 75 amino acids, about 10 to 50 amino acids, about 10 to 40 amino acids, about 10 to 30 amino acids, about 10 to 20 amino acids, or about 10 to 15 amino acids, and including any integer between the endpoints of any of the listed ranges. In some embodiments, a spacer region has about 12 amino acids or less, about 119 amino acids or less, or about 229 amino acids or less. Exemplary spacers include IgG4 hinge alone, IgG4 hinge linked to CH2 and CH3 domains, or IgG4 hinge linked to the CH3 domain. Exemplary spacers include, but are not limited to, those described in Hudecek et al. (2013) Clin. Cancer Res., 19:3153 or international patent application publication number WO2014031687. In some embodiments, the constant region or portion is of IgD. 
     The antigen recognition domain of a receptor such as a TCR or CAR can be linked to one or more intracellular signaling components, such as signaling components that mimic activation through an antigen receptor complex, such as a TCR complex, in the case of a CAR, and/or signal via another cell surface receptor. Thus, in some embodiments, the HLA-PEPTIDE-specific binding component (e.g., ABP such as antibody or TCR) is linked to one or more transmembrane and intracellular signaling domains. In some embodiments, the transmembrane domain is fused to the extracellular domain. In one embodiment, a transmembrane domain that naturally is associated with one of the domains in the receptor, e.g., CAR, is used. In some instances, the transmembrane domain is selected or modified by amino acid substitution to avoid binding of such domains to the transmembrane domains of the same or different surface membrane proteins to minimize interactions with other members of the receptor complex. 
     The transmembrane domain in some embodiments is derived either from a natural or from a synthetic source. Where the source is natural, the domain in some aspects is derived from any membrane-bound or transmembrane protein. Transmembrane regions include those derived from (i.e. comprise at least the transmembrane region(s) of) the alpha, beta or zeta chain of the T-cell receptor, CD28, CD3 epsilon, CD45, CD4, CD5, CDS, CD9, CD 16, CD22, CD33, CD37, CD64, CD80, CD86, CD 134, CD137, and/or CD 154. Alternatively the transmembrane domain in some embodiments is synthetic. In some aspects, the synthetic transmembrane domain comprises predominantly hydrophobic residues such as leucine and valine. In some aspects, a triplet of phenylalanine, tryptophan and valine will be found at each end of a synthetic transmembrane domain. In some embodiments, the linkage is by linkers, spacers, and/or transmembrane domain(s). 
     Among the intracellular signaling domains are those that mimic or approximate a signal through a natural antigen receptor, a signal through such a receptor in combination with a costimulatory receptor, and/or a signal through a costimulatory receptor alone. In some embodiments, a short oligo- or polypeptide linker, for example, a linker of between 2 and 10 amino acids in length, such as one containing glycines and serines, e.g., glycine-serine doublet, is present and forms a linkage between the transmembrane domain and the cytoplasmic signaling domain of the receptor. 
     The receptor, e.g., the TCR or CAR, can include at least one intracellular signaling component or components. In some embodiments, the receptor includes an intracellular component of a TCR complex, such as a TCR CD3 chain that mediates T-cell activation and cytotoxicity, e.g., CD3 zeta chain. Thus, in some aspects, the HLA-PEPTIDE-binding ABP (e.g., antibody) is linked to one or more cell signaling modules. In some embodiments, cell signaling modules include CD3 transmembrane domain, CD3 intracellular signaling domains, and/or other CD transmembrane domains. In some embodiments, the receptor, e.g., CAR, further includes a portion of one or more additional molecules such as Fc receptor-gamma, CD8, CD4, CD25, or CD16. For example, in some aspects, the CAR includes a chimeric molecule between CD3-zeta or Fc receptor-gamma and CD8, CD4, CD25 or CD16. 
     In some embodiments, upon ligation of the TCR or CAR, the cytoplasmic domain or intracellular signaling domain of the receptor activates at least one of the normal effector functions or responses of the immune cell, e.g., T cell engineered to express the receptor. For example, in some contexts, the receptor induces a function of a T cell such as cytolytic activity or T-helper activity, such as secretion of cytokines or other factors. In some embodiments, a truncated portion of an intracellular signaling domain of an antigen receptor component or costimulatory molecule is used in place of an intact immunostimulatory chain, for example, if it transduces the effector function signal. In some embodiments, the intracellular signaling domain or domains include the cytoplasmic sequences of the T cell receptor (TCR), and in some aspects also those of co-receptors that in the natural context act in concert with such receptor to initiate signal transduction following antigen receptor engagement, and/or any derivative or variant of such molecules, and/or any synthetic sequence that has the same functional capability. 
     In the context of a natural TCR, full activation generally uses not only signaling through the TCR, but also a costimulatory signal. Thus, in some embodiments, to promote full activation, a component for generating secondary or co-stimulatory signal is also included in the receptor. In other embodiments, the receptor does not include a component for generating a costimulatory signal. In some aspects, an additional receptor is expressed in the same cell and provides the component for generating the secondary or costimulatory signal. 
     T cell activation is in some aspects described as being mediated by two classes of cytoplasmic signaling sequences: those that initiate antigen-dependent primary activation through the TCR (primary cytoplasmic signaling sequences), and those that act in an antigen-independent manner to provide a secondary or co-stimulatory signal (secondary cytoplasmic signaling sequences). In some aspects, the receptor includes one or both of such signaling components. 
     In some aspects, the receptor includes a primary cytoplasmic signaling sequence that regulates primary activation of the TCR complex. Primary cytoplasmic signaling sequences that act in a stimulatory manner may contain signaling motifs which are known as immunoreceptor tyrosine-based activation motifs or ITAMs. Examples of ITAM containing primary cytoplasmic signaling sequences include those derived from TCR or CD3 zeta, FcR gamma, FcR beta, CD3 gamma, CD3 delta, CD3 epsilon, CDS, CD22, CD79a, CD79b, and CD66d. In some embodiments, cytoplasmic signaling molecule(s) in the CAR contain(s) a cytoplasmic signaling domain, portion thereof, or sequence derived from CD3 zeta. 
     In some embodiments, the receptor includes a signaling domain and/or transmembrane portion of a costimulatory receptor, such as CD28, 4-1BB, OX40, DAP10, and ICOS. In some aspects, the same receptor includes both the activating and costimulatory components. 
     In some embodiments, the activating domain is included within one receptor, whereas the costimulatory component is provided by another receptor recognizing another antigen. In some embodiments, the receptors include activating or stimulatory receptors, and costimulatory receptors, both expressed on the same cell (see WO2014/055668). In some aspects, the HLA-PEPTIDE-targeting receptor is the stimulatory or activating receptor; in other aspects, it is the costimulatory receptor. In some embodiments, the cells further include inhibitory receptors (e.g., iCARs, see Fedorov et al., Sci. Transl. Medicine, 5(215) (December, 2013), such as a receptor recognizing an antigen other than HLA-PEPTIDE, whereby an activating signal delivered through the HLA-PEPTIDE-targeting receptor is diminished or inhibited by binding of the inhibitory receptor to its ligand, e.g., to reduce off-target effects. 
     In certain embodiments, the intracellular signaling domain comprises a CD28 transmembrane and signaling domain linked to a CD3 (e.g., CD3-zeta) intracellular domain. In some embodiments, the intracellular signaling domain comprises a chimeric CD28 and CD137 (4-1BB, TNFRSF9) co-stimulatory domains, linked to a CD3 zeta intracellular domain. 
     In some embodiments, the receptor encompasses one or more, e.g., two or more, costimulatory domains and an activation domain, e.g., primary activation domain, in the cytoplasmic portion. Exemplary receptors include intracellular components of CD3-zeta, CD28, and 4-1BB. 
     In some embodiments, the CAR or other antigen receptor such as a TCR further includes a marker, such as a cell surface marker, which may be used to confirm transduction or engineering of the cell to express the receptor, such as a truncated version of a cell surface receptor, such as truncated EGFR (tEGFR). In some aspects, the marker includes all or part (e.g., truncated form) of CD34, a nerve growth factor receptor (NGFR), or epidermal growth factor receptor (e.g., tEGFR). In some embodiments, the nucleic acid encoding the marker is operably linked to a polynucleotide encoding for a linker sequence, such as a cleavable linker sequence or a ribosomal skip sequence, e.g., T2A. See WO2014031687. In some embodiments, introduction of a construct encoding the CAR and EGFRt separated by a T2A ribosome switch can express two proteins from the same construct, such that the EGFRt can be used as a marker to detect cells expressing such construct. In some embodiments, a marker, and optionally a linker sequence, can be any as disclosed in published patent application No. WO2014031687. For example, the marker can be a truncated EGFR (tEGFR) that is, optionally, linked to a linker sequence, such as a T2A ribosomal skip sequence. 
     In some embodiments, the marker is a molecule, e.g., cell surface protein, not naturally found on T cells or not naturally found on the surface of T cells, or a portion thereof. 
     In some embodiments, the molecule is a non-self molecule, e.g., non-self protein, i.e., one that is not recognized as “self” by the immune system of the host into which the cells will be adoptively transferred. 
     In some embodiments, the marker serves no therapeutic function and/or produces no effect other than to be used as a marker for genetic engineering, e.g., for selecting cells successfully engineered. In other embodiments, the marker may be a therapeutic molecule or molecule otherwise exerting some desired effect, such as a ligand for a cell to be encountered in vivo, such as a costimulatory or immune checkpoint molecule to enhance and/or dampen responses of the cells upon adoptive transfer and encounter with ligand. 
     The TCR or CAR may comprise one or modified synthetic amino acids in place of one or more naturally-occurring amino acids. Exemplary modified amino acids include, but are not limited to, aminocyclohexane carboxylic acid, norleucine, α-amino n-decanoic acid, homoserine, S-acetylaminomethylcysteine, trans-3- and trans-4-hydroxyproline, 4-aminophenylalanine, 4-nitrophenylalanine, 4-chlorophenylalanine, 4-carboxyphenylalanine, (3-phenylserine (3-hydroxyphenylalanine, phenylglycine, α-naphthylalanine, cyclohexylalanine, cyclohexylglycine, indoline-2-carboxylic acid, 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid, aminomalonic acid, aminomalonic acid monoamide, N′-benzyl-N′-methyl-lysine, N′,N′-dibenzyl-lysine, 6-hydroxylysine, ornithine, α-aminocyclopentane carboxylic acid, α-aminocyclohexane carboxylic acid, α-aminocycloheptane carboxylic acid, α-(2-amino-2-norbornane)-carboxylic acid, α,γ-diaminobutyric acid, α,γ-diaminopropionic acid, homophenylalanine, and α-tertbutylglycine. 
     In some cases, CARs are referred to as first, second, and/or third generation CARs. In some aspects, a first generation CAR is one that solely provides a CD3-chain induced signal upon antigen binding; in some aspects, a second-generation CARs is one that provides such a signal and costimulatory signal, such as one including an intracellular signaling domain from a costimulatory receptor such as CD28 or CD137; in some aspects, a third generation CAR in some aspects is one that includes multiple costimulatory domains of different costimulatory receptors. 
     In some embodiments, the chimeric antigen receptor includes an extracellular portion containing an antibody or fragment described herein. In some aspects, the chimeric antigen receptor includes an extracellular portion containing an antibody or fragment described herein and an intracellular signaling domain. In some embodiments, an antibody or fragment includes an scFv or a single-domain VH antibody and the intracellular domain contains an ITAM. In some aspects, the intracellular signaling domain includes a signaling domain of a zeta chain of a CD3-zeta (CD3) chain. In some embodiments, the chimeric antigen receptor includes a transmembrane domain linking the extracellular domain and the intracellular signaling domain. 
     In some aspects, the transmembrane domain contains a transmembrane portion of CD28. The extracellular domain and transmembrane can be linked directly or indirectly. In some embodiments, the extracellular domain and transmembrane are linked by a spacer, such as any described herein. In some embodiments, the chimeric antigen receptor contains an intracellular domain of a T cell costimulatory molecule, such as between the transmembrane domain and intracellular signaling domain. In some aspects, the T cell costimulatory molecule is CD28 or 41BB. 
     In some embodiments, the CAR contains an antibody, e.g., an antibody fragment, a transmembrane domain that is or contains a transmembrane portion of CD28 or a functional variant thereof, and an intracellular signaling domain containing a signaling portion of CD28 or functional variant thereof and a signaling portion of CD3 zeta or functional variant thereof. In some embodiments, the CAR contains an antibody, e.g., antibody fragment, a transmembrane domain that is or contains a transmembrane portion of CD28 or a functional variant thereof, and an intracellular signaling domain containing a signaling portion of a 4-1BB or functional variant thereof and a signaling portion of CD3 zeta or functional variant thereof. In some such embodiments, the receptor further includes a spacer containing a portion of an Ig molecule, such as a human Ig molecule, such as an Ig hinge, e.g. an IgG4 hinge, such as a hinge-only spacer. 
     In some embodiments, the transmembrane domain of the receptor, e.g., the CAR, is a transmembrane domain of human CD28 or variant thereof, e.g., a 27-amino acid transmembrane domain of a human CD28 (Accession No.: P10747.1). 
     In some embodiments, the chimeric antigen receptor contains an intracellular domain of a T cell costimulatory molecule. In some aspects, the T cell costimulatory molecule is CD28 or 41BB. 
     In some embodiments, the intracellular signaling domain comprises an intracellular costimulatory signaling domain of human CD28 or functional variant or portion thereof, such as a 41 amino acid domain thereof and/or such a domain with an LL to GG substitution at positions 186-187 of a native CD28 protein. In some embodiments, the intracellular domain comprises an intracellular costimulatory signaling domain of 41BB or functional variant or portion thereof, such as a 42-amino acid cytoplasmic domain of a human 4-1BB (Accession No. Q07011.1) or functional variant or portion thereof. 
     In some embodiments, the intracellular signaling domain comprises a human CD3 zeta stimulatory signaling domain or functional variant thereof, such as a 112 AA cytoplasmic domain of isoform 3 of human CD3.zeta. (Accession No.: P20963.2) or a CD3 zeta signaling domain as described in U.S. Pat. No. 7,446,190 or 8,911,993. 
     In some aspects, the spacer contains only a hinge region of an IgG, such as only a hinge of IgG4 or IgG1. In other embodiments, the spacer is an Ig hinge, e.g., and IgG4 hinge, linked to a CH2 and/or CH3 domains. In some embodiments, the spacer is an Ig hinge, e.g., an IgG4 hinge, linked to CH2 and CH3 domains. In some embodiments, the spacer is an Ig hinge, e.g., an IgG4 hinge, linked to a CH3 domain only. In some embodiments, the spacer is or comprises a glycine-serine rich sequence or other flexible linker such as known flexible linkers. 
     For example, in some embodiments, the CAR includes an antibody or fragment thereof, such as any of the HLA-PEPTIDE antibodies, including single chain antibodies (sdAbs, e.g. containing only the VH region) and scFvs, described herein, a spacer such as any of the Ig-hinge containing spacers, a CD28 transmembrane domain, a CD28 intracellular signaling domain, and a CD3 zeta signaling domain. In some embodiments, the CAR includes an antibody or fragment, such as any of the HLA-PEPTIDE antibodies, including sdAbs and scFvs described herein, a spacer such as any of the Ig-hinge containing spacers, a CD28 transmembrane domain, a CD28 intracellular signaling domain, and a CD3 zeta signaling domain. 
     Target-Specific TCRs to A*01:01 ASSLPTTMNY [G10] 
     In some aspects, provided herein are ABPs comprising TCRs or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*01:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises the sequence ASSLPTTMNY (“G10”). 
     The TCR specific for A*01:01_ASSLPTTMNY may comprise an αCDR3 sequence. The αCDR3 sequence may be any one of the αCDR3 sequences in Table 15. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. Alpha and beta CDR3 sequences of the identified TCR clonotypes are shown in Table 15. 
     The TCR specific for A*01:01_ASSLPTTMNY may comprise a βCDR3 sequence. The βCDR3 sequence may be any one of the βCDR3 sequences in Table 15. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_ASSLPTTMNY may comprise a particular αCDR3 sequence and a particular βCDR3 sequence. For example, the TCR specific for A*01:01_ASSLPTTMNY may comprise the αCDR3 sequence and βCDR3 sequence from any one of TCRs identified in Table 15. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_ASSLPTTMNY may comprise a TRAV, a TRAJ, a TRBV, optionally a TRBD, and a TRBJ amino acid sequence, optionally a TRAC sequence and optionally a TRBC sequence. For example, the TCR specific for A*01:01_ASSLPTTMNY may comprise the TRAV, TRAJ, TRBV, TRBD, TRBJ amino acid sequence, TRAC sequence and TRBC sequence from any one of the TCRs identified in Table 14. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. For clarity, each identified TCR was assigned a TCR ID number. For example the TCR assigned TCR ID #1 comprises a TRAV25 sequence, a TRAJ37 sequence, a TRAC sequence, a TRBV19 sequence, a TRBD1 sequence, a TRBJ1-5 sequence, and a TRBC1 sequence. 
     The TCR specific for A*01:01_ASSLPTTMNY may comprise an alpha VJ sequence. The alpha VJ sequence may be any one of the alpha VJ sequences in Table 16. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_ASSLPTTMNY may comprise a beta V(D)J sequence. The beta V(D)J sequence may be any one of the beta V(D)J sequences in Table 16. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_ASSLPTTMNY may comprise an alpha VJ sequence and a beta V(D)J sequence. For example, the TCR specific for A*01:01_ASSLPTTMNY may comprise the alpha VJ sequence and the beta V(D)J sequence from any one of the TCRs identified in Table 16. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. Full length alpha V(J) and beta V(D)J sequences of the identified TCR clonotypes are shown in Table 16. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     Target-Specific TCRs to A*01:01 HSEVGLPVY 
     In some aspects, provided herein are ABPs comprising TCRs or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*01:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises the sequence HSEVGLPVY 
     The TCR specific for A*01:01_HSEVGLPVY may comprise an αCDR3 sequence. The αCDR3 sequence may be any one of the αCDR3 sequences in Table 18. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. Alpha and beta CDR3 sequences of the identified TCR clonotypes are shown in Table 18. 
     The TCR specific for A*01:01_HSEVGLPVY may comprise a βCDR3 sequence. The βCDR3 sequence may be any one of the βCDR3 sequences in Table 18. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_HSEVGLPVY may comprise a particular αCDR3 sequence and a particular βCDR3 sequence. For example, the TCR specific for A*01:01_HSEVGLPVY may comprise the αCDR3 sequence and βCDR3 sequence from any one of TCRs identified in Table 18. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_HSEVGLPVY may comprise a TRAV, a TRAJ, a TRBV, optionally a TRBD, and a TRBJ amino acid sequence, optionally a TRAC sequence and optionally a TRBC sequence. For example, the TCR specific for A*01:01_HSEVGLPVY may comprise the TRAV, TRAJ, TRBV, TRBD, TRBJ amino acid sequence, TRAC sequence and TRBC sequence from any one of the TCRs identified in Table 17. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_HSEVGLPVY may comprise an alpha VJ sequence. The alpha VJ sequence may be any one of the alpha VJ sequences in Table 19. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_HSEVGLPVY may comprise a beta V(D)J sequence. The beta V(D)J sequence may be any one of the beta V(D)J sequences in Table 19. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     The TCR specific for A*01:01_HSEVGLPVY may comprise an alpha VJ sequence and a beta V(D)J sequence. For example, the TCR specific for A*01:01_HSEVGLPVY may comprise the alpha VJ sequence and the beta V(D)J sequence from any one of the TCRs identified in Table 19. Refer to PCT/US2018/06793, filed on Dec. 28, 2018, which is hereby incorporated by reference in its entirety. 
     Target-Specific TCRs to A*02:01 LLASSILCA [G7] 
     In some aspects, provided herein are ABPs comprising TCRs or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*02:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises the sequence LLASSILCA. 
     The TCR specific for A*02:01__LLASSILCA may comprise an αCDR3 sequence. Refer to SEQ ID NO: 4277, 4278, 4279, 4280, or 4281 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01__LLASSILCA may comprise a βCDR3 sequence. Refer to SEQ ID NOS 4291-4295 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01__LLASSILCA may comprise a particular αCDR3 sequence and a particular βCDR3 sequence. For particular combinations of αCDR3 and βCDR3 sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01__LLASSILCA may comprise a TRAV, a TRAJ, a TRBV, optionally a TRBD, and a TRBJ amino acid sequence, optionally a TRAC sequence and optionally a TRBC sequence. For particular combinations of TRAV, TRAJ, TRBV, optionally TRBD, TRBJ amino acid sequence, optionally TRAC sequence and optionally TRBC sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01__LLASSILCA may comprise an alpha VJ sequence. Refer to SEQ ID NOS 4306-4310 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01__LLASSILCA may comprise a beta V(D)J sequence. Refer to SEQ ID NOS 4321-4325 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. The TCR specific for A*02:01__LLASSILCA may comprise an alpha VJ sequence and a beta V(D)J sequence. For particular combinations of alpha VJ and beta V(D)J sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     Target-Specific TCRs to A*01:01 EVDPIGHLY 
     In some aspects, provided herein are ABPs comprising TCRs or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*01:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises the sequence EVDPIGHLY. 
     The TCR specific for A*01:01_EVDPIGHLY may comprise an αCDR3 sequence. Refer to SEQ ID NOS 3052-3350 or 4273-4276 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*01:01_EVDPIGHLY may comprise a βCDR3 sequence. Refer to SEQ ID NOS 3351-3655 or 4287-4290 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety 
     The TCR specific for A*01:01_EVDPIGHLY may comprise a particular αCDR3 sequence and a particular βCDR3 sequence. For particular combinations of αCDR3 and βCDR3 sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*01:01_EVDPIGHLY may comprise a TRAV, a TRAJ, a TRBV, optionally a TRBD, and a TRBJ amino acid sequence, optionally a TRAC sequence and optionally a TRBC sequence. For particular combinations of TRAV, TRAJ, TRBV, optionally TRBD, TRBJ amino acid sequence, optionally TRAC sequence and optionally TRBC sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*01:01_EVDPIGHLY may comprise an alpha VJ sequence. Refer to SEQ ID NOS 3656-3961 or 4302-4305 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*01:01_EVDPIGHLY may comprise a beta V(D)J sequence. Refer to SEQ ID NOS 3962-4269 or 4317-4320 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*01:01_EVDPIGHLY may comprise an alpha VJ sequence and a beta V(D)J sequence. For particular combinations of alpha VJ and beta V(D)J sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     Target-Specific TCRs to B*44:02 GEMSSNSTAL 
     In some aspects, provided herein are ABPs comprising TCRs or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype B*44:02 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises the sequence GEMSSNSTAL. 
     The TCR specific for B*44:02_GEMSSNSTAL may comprise an αCDR3 sequence. Refer to SEQ ID NOS 4284-4286 or 3138 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for B*44:02_GEMSSNSTAL may comprise a βCDR3 sequence. Refer to SEQ ID NOS 4298-4301 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for B*44:02_GEMSSNSTAL may comprise a particular αCDR3 sequence and a particular βCDR3 sequence. For particular combinations of αCDR3 and βCDR3 sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for B*44:02_GEMSSNSTAL may comprise a TRAV, a TRAJ, a TRBV, optionally a TRBD, and a TRBJ amino acid sequence, optionally a TRAC sequence and optionally a TRBC sequence. For particular combinations of TRAV, TRAJ, TRBV, optionally TRBD, TRBJ amino acid sequence, optionally TRAC sequence and optionally TRBC sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for B*44:02_GEMSSNSTAL may comprise an alpha VJ sequence. Refer to SEQ ID NOS 4313-4316 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for B*44:02_GEMSSNSTAL may comprise a beta V(D)J sequence. Refer to SEQ ID NOS 4328-4331 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for B*44:02_GEMSSNSTAL may comprise an alpha VJ sequence and a beta V(D)J sequence. For particular combinations of alpha VJ and beta V(D)J sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     Target-Specific TCRs to A*02:01 GVYDGEEHSV 
     In some aspects, provided herein are ABPs comprising TCRs or antigen-binding fragments thereof that specifically bind an HLA-PEPTIDE target, wherein the HLA Class I molecule of the HLA-PEPTIDE target is HLA subtype A*02:01 and the HLA-restricted peptide of the HLA-PEPTIDE target comprises the sequence GVYDGEEHSV. 
     The TCR specific for A*02:01_GVYDGEEHSV may comprise an αCDR3 sequence. Refer to SEQ ID NOS 4282-4283 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01 GVYDGEEHSV may comprise a βCDR3 sequence. Refer to SEQ ID NOS 4296-4297 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01_GVYDGEEHSV may comprise a particular αCDR3 sequence and a particular βCDR3 sequence. For particular combinations of αCDR3 and βCDR3 sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01_GVYDGEEHSV may comprise a TRAV, a TRAJ, a TRBV, optionally a TRBD, and a TRBJ amino acid sequence, optionally a TRAC sequence and optionally a TRBC sequence. For particular combinations of TRAV, TRAJ, TRBV, optionally TRBD, TRBJ amino acid sequence, optionally TRAC sequence and optionally TRBC sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01_GVYDGEEHSV may comprise an alpha VJ sequence. Refer to SEQ ID NOS 4311-4312 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01_GVYDGEEHSV may comprise a beta V(D)J sequence. Refer to SEQ ID NOS 4326-4327 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     The TCR specific for A*02:01_GVYDGEEHSV may comprise an alpha VJ sequence and a beta V(D)J sequence. For particular combinations of alpha VJ and beta V(D)J sequences, refer to PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     Engineered Cells 
     Also provided are cells such as cells that contain an antigen receptor, e.g., that contains an extracellular domain including an anti-HLA-PEPTIDE ABP (e.g., a CAR or TCR), described herein. Also provided are populations of such cells, and compositions containing such cells. In some embodiments, compositions or populations are enriched for such cells, such as in which cells expressing the HLA-PEPTIDE ABP make up at least 1, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or more than 99 percent of the total cells in the composition or cells of a certain type such as T cells or CD8+ or CD4+ cells. In some embodiments, a composition comprises at least one cell containing an antigen receptor disclosed herein. Among the compositions are pharmaceutical compositions and formulations for administration, such as for adoptive cell therapy. Also provided are therapeutic methods for administering the cells and compositions to subjects, e.g., patients. 
     Thus also provided are genetically engineered cells expressing an ABP comprising a receptor, e.g., a TCR or CAR. The cells generally are eukaryotic cells, such as mammalian cells, and typically are human cells. In some embodiments, the cells are derived from the blood, bone marrow, lymph, or lymphoid organs, are cells of the immune system, such as cells of the innate or adaptive immunity, e.g., myeloid or lymphoid cells, including lymphocytes, typically T cells and/or NK cells. Other exemplary cells include stem cells, such as multipotent and pluripotent stem cells, including induced pluripotent stem cells (iPSCs). The cells typically are primary cells, such as those isolated directly from a subject and/or isolated from a subject and frozen. In some embodiments, the cells include one or more subsets of T cells or other cell types, such as whole T cell populations, CD4+ cells, CD8+ cells, and subpopulations thereof, such as those defined by function, activation state, maturity, potential for differentiation, expansion, recirculation, localization, and/or persistence capacities, antigen-specificity, type of antigen receptor, presence in a particular organ or compartment, marker or cytokine secretion profile, and/or degree of differentiation. With reference to the subject to be treated, the cells may be allogeneic and/or autologous. Among the methods include off-the-shelf methods. In some aspects, such as for off-the-shelf technologies, the cells are pluripotent and/or multipotent, such as stem cells, such as induced pluripotent stem cells (iPSCs). In some embodiments, the methods include isolating cells from the subject, preparing, processing, culturing, and/or engineering them, as described herein, and re-introducing them into the same patient, before or after cryopreservation. 
     Among the sub-types and subpopulations of T cells and/or of CD4+ and/or of CD8+ T cells are naive T (TN) cells, effector T cells (TEFF), memory T cells and sub-types thereof, such as stem cell memory T (TSCM), central memory T (TCM), effector memory T (TEM), or terminally differentiated effector memory T cells, tumor-infiltrating lymphocytes (TIL), immature T cells, mature T cells, helper T cells, cytotoxic T cells, mucosa-associated invariant T (MALT) cells, naturally occurring and adaptive regulatory T (Treg) cells, helper T cells, such as TH1 cells, TH2 cells, TH3 cells, TH17 cells, TH9 cells, TH22 cells, follicular helper T cells, alpha/beta T cells, and delta/gamma T cells. 
     In some embodiments, the cells are natural killer (NK) cells. In some embodiments, the cells are monocytes or granulocytes, e.g., myeloid cells, macrophages, neutrophils, dendritic cells, mast cells, eosinophils, and/or basophils. 
     The cells may be genetically modified to reduce expression or knock out endogenous TCRs. Such modifications are described in Mol Ther Nucleic Acids. 2012 December; 1(12): e63; Blood. 2011 Aug. 11; 118(6):1495-503; Blood. 2012 Jun. 14; 119(24): 5697-5705; Torikai, Hiroki et al “HLA and TCR Knockout by Zinc Finger Nucleases: Toward “off-the-Shelf” Allogeneic T-Cell Therapy for CD19+ Malignancies.” Blood 116.21 (2010): 3766; Blood. 2018 Jan. 18; 131(3):311-322. doi: 10.1182/blood-2017-05-787598; and WO2016069283, which are incorporated by reference in their entirety. 
     The cells may be genetically modified to promote cytokine secretion. Such modifications are described in Hsu C, Hughes M S, Zheng Z, Bray R B, Rosenberg S A, Morgan R A. Primary human T lymphocytes engineered with a codon-optimized IL-15 gene resist cytokine withdrawal-induced apoptosis and persist long-term in the absence of exogenous cytokine. J Immunol. 2005; 175:7226-34; Quintarelli C, Vera J F, Savoldo B, Giordano Attianese G M, Pule M, Foster A E, Co-expression of cytokine and suicide genes to enhance the activity and safety of tumor-specific cytotoxic T lymphocytes. Blood. 2007; 110:2793-802; and Hsu C, Jones S A, Cohen C J, Zheng Z, Kerstann K, Zhou J, Cytokine-independent growth and clonal expansion of a primary human CD8+ T-cell clone following retroviral transduction with the IL-15 gene. Blood. 2007; 109:5168-77. 
     Mismatching of chemokine receptors on T cells and tumor-secreted chemokines has been shown to account for the suboptimal trafficking of T cells into the tumor microenvironment. To improve efficacy of therapy, the cells may be genetically modified to increase recognition of chemokines in tumor micro environment. Examples of such modifications are described in Moon et al., Expression of a functional CCR2 receptor enhances tumor localization and tumor eradication by retargeted human T cells expressing a mesothelin-specific chimeric antibody receptor, Clin Cancer Res. 2011; 17: 4719-4730; and Craddock et al., Enhanced tumor trafficking of GD2 chimeric antigen receptor T cells by expression of the chemokine receptor CCR2b. J Immunother. 2010; 33: 780-788. 
     The cells may be genetically modified to enhance expression of costimulatory/enhancing receptors, such as CD28 and 41BB. 
     Adverse effects of T cell therapy can include cytokine release syndrome and prolonged B-cell depletion. Introduction of a suicide/safety switch in the recipient cells may improve the safety profile of a cell-based therapy. Accordingly, the cells may be genetically modified to include a suicide/safety switch. The suicide/safety switch may be a gene that confers sensitivity to an agent, e.g., a drug, upon the cell in which the gene is expressed, and which causes the cell to die when the cell is contacted with or exposed to the agent. Exemplary suicide/safety switches are described in Protein Cell. 2017 August; 8(8): 573-589. The suicide/safety switch may be HSV-TK. The suicide/safety switch may be cytosine deaminase, purine nucleoside phosphorylase, or nitroreductase. The suicide/safety switch may be RapaCIDe™, described in U.S. Patent Application Pub. No. US20170166877A1. The suicide/safety switch system may be CD20/Rituximab, described in Haematologica. 2009 September; 94(9): 1316-1320. These references are incorporated by reference in their entirety. 
     The TCR or CAR may be introduced into the recipient cell as a split receptor which assembles only in the presence of a heterodimerizing small molecule. Such systems are described in Science. 2015 Oct. 16; 350(6258): aab4077, and in U.S. Pat. No. 9,587,020, which are hereby incorporated by reference. 
     In some embodiments, the cells include one or more nucleic acids, e.g., a polynucleotide encoding a TCR or CAR disclosed herein, wherein the polynucleotide is introduced via genetic engineering, and thereby express recombinant or genetically engineered TCRs or CARs as disclosed herein. In some embodiments, the nucleic acids are heterologous, i.e., normally not present in a cell or sample obtained from the cell, such as one obtained from another organism or cell, which for example, is not ordinarily found in the cell being engineered and/or an organism from which such cell is derived. In some embodiments, the nucleic acids are not naturally occurring, such as a nucleic acid not found in nature, including one comprising chimeric combinations of nucleic acids encoding various domains from multiple different cell types. 
     The nucleic acids may include a codon-optimized nucleotide sequence. Without being bound to a particular theory or mechanism, it is believed that codon optimization of the nucleotide sequence increases the translation efficiency of the mRNA transcripts. Codon optimization of the nucleotide sequence may involve substituting a native codon for another codon that encodes the same amino acid, but can be translated by tRNA that is more readily available within a cell, thus increasing translation efficiency. Optimization of the nucleotide sequence may also reduce secondary mRNA structures that would interfere with translation, thus increasing translation efficiency. 
     A construct or vector may be used to introduce the TCR or CAR into the recipient cell. Exemplary constructs are described herein. Polynucleotides encoding the alpha and beta chains of the TCR or CAR may in a single construct or in separate constructs. The polynucleotides encoding the alpha and beta chains may be operably linked to a promoter, e.g., a heterologous promoter. The heterologous promoter may be a strong promoter, e.g., EF1alpha, CMV, PGK1, Ubc, beta actin, CAG promoter, and the like. The heterologous promoter may be a weak promoter. The heterologous promoter may be an inducible promoter. Exemplary inducible promoters include, but are not limited to TRE, NFAT, GAL4, LAC, and the like. Other exemplary inducible expression systems are described in U.S. Pat. Nos. 5,514,578; 6,245,531; 7,091,038 and European Patent No. 0517805, which are incorporated by reference in their entirety. 
     The construct for introducing the TCR or CAR into the recipient cell may also comprise a polynucleotide encoding a signal peptide (signal peptide element). The signal peptide may promote surface trafficking of the introduced TCR or CAR. Exemplary signal peptides include, but are not limited to CD8 signal peptide, immunoglobulin signal peptides, where specific examples include GM-CSF and IgG kappa. Such signal peptides are described in Trends Biochem Sci. 2006 October; 31(10):563-71. Epub 2006 Aug. 21; and An, et al. “Construction of a New Anti-CD19 Chimeric Antigen Receptor and the Anti-Leukemia Function Study of the Transduced T Cells.” Oncotarget 7.9 (2016): 10638-10649. PMC. Web. 16 Aug. 2018; which are hereby incorporated by reference. 
     In some cases, e.g., cases where the alpha and beta chains are expressed from a single construct or open reading frame, or cases wherein a marker gene is included in the construct, the construct may comprise a ribosomal skip sequence. The ribosomal skip sequence may be a 2A peptide, e.g., a P2A or T2A peptide. Exemplary P2A and T2A peptides are described in Scientific Reports volume 7, Article number: 2193 (2017), hereby incorporated by reference in its entirety. In some cases, a FURIN/PACE cleavage site is introduced upstream of the 2A element. FURIN/PACE cleavage sites are described in, e.g., http://www.nuolan.net/substrates.html. The cleavage peptide may also be a factor Xa cleavage site. In cases where the alpha and beta chains are expressed from a single construct or open reading frame, the construct may comprise an internal ribosome entry site (IRES). 
     The construct may further comprise one or more marker genes. Exemplary marker genes include but are not limited to GFP, luciferase, HA, lacZ. The marker may be a selectable marker, such as an antibiotic resistance marker, a heavy metal resistance marker, or a biocide resistant marker, as is known to those of skill in the art. The marker may be a complementation marker for use in an auxotrophic host. Exemplary complementation markers and auxotrophic hosts are described in Gene. 2001 Jan. 24; 263(1-2):159-69. Such markers may be expressed via an IRES, a frameshift sequence, a 2A peptide linker, a fusion with the TCR or CAR, or expressed separately from a separate promoter. 
     Exemplary vectors or systems for introducing TCRs or CARs into recipient cells include, but are not limited to Adeno-associated virus, Adenovirus, Adenovirus+Modified vaccinia, Ankara virus (MVA), Adenovirus+Retrovirus, Adenovirus+Sendai virus, Adenovirus+Vaccinia virus, Alphavirus (VEE) Replicon Vaccine, Antisense oligonucleotide,  Bifidobacterium longum , CRISPR-Cas9,  E. coli , Flavivirus, Gene gun, Herpesviruses, Herpes simplex virus,  Lactococcus lactis , Electroporation, Lentivirus, Lipofection,  Listeria monocytogenes , Measles virus, Modified Vaccinia Ankara virus (MVA), mRNA Electroporation, Naked/Plasmid DNA, Naked/Plasmid DNA+Adenovirus, Naked/Plasmid DNA+Modified Vaccinia Ankara virus (MVA), Naked/Plasmid DNA+RNA transfer, Naked/Plasmid DNA+Vaccinia virus, Naked/Plasmid DNA+Vesicular stomatitis virus, Newcastle disease virus, Non-viral, PiggyBacm (PB) Transposon, nanoparticle-based systems, Poliovirus, Poxvirus, Poxvirus+Vaccinia virus, Retrovirus, RNA transfer, RNA transfer+Naked/Plasmid DNA, RNA virus,  Saccharomyces cerevisiae, Salmonella typhimurium , Semliki forest virus, Sendai virus,  Shigella dysenteriae , Simian virus, siRNA, Sleeping Beauty transposon,  Streptococcus mutans , Vaccinia virus, Venezuelan equine encephalitis virus replicon, Vesicular stomatitis virus, and  Vibrio cholera.    
     In preferred embodiments, the TCR or CAR is introduced into the recipient cell via adeno associated virus (AAV), adenovirus, CRISPR-CAS9, herpesvirus, lentivirus, lipofection, mRNA electroporation, PiggyBacm (PB) Transposon, retrovirus, RNA transfer, or Sleeping Beauty transposon. 
     In some embodiments, a vector for introducing a TCR or CAR into a recipient cell is a viral vector. Exemplary viral vectors include adenoviral vectors, adeno-associated viral (AAV) vectors, lentiviral vectors, herpes viral vectors, retroviral vectors, and the like. Such vectors are described herein. 
     Exemplary embodiments of TCR constructs for introducing a TCR or CAR into recipient cells is shown in  FIG. 2 . In some embodiments, a TCR construct includes, from the 5′-3′ direction, the following polynucleotide sequences: a promoter sequence, a signal peptide sequence, a TCR β variable (TCRβv) sequence, a TCR β constant ((TCRβc) sequence, a cleavage peptide (e.g., P2A), a signal peptide sequence, a TCR α variable (TCRαv) sequence, and a TCR α constant (TCRαc) sequence. In some embodiments, the TCRβc and TCRαc sequences of the construct include one or more murine regions, e.g., full murine constant sequences or human→murine amino acid exchanges as described herein. In some embodiments, the construct further includes, 3′ of the TCRαc sequence, a cleavage peptide sequence (e.g., T2A) followed by a reporter gene. In an embodiment, the construct includes, from the 5′-3′ direction, the following polynucleotide sequences: a promoter sequence, a signal peptide sequence, a TCR β variable (TCRβv) sequence, a TCR β constant ((TCRβc) sequence containing one or more murine regions, a cleavage peptide (e.g., P2A), a signal peptide sequence, a TCR α variable (TCRαv) sequence, and a TCR α constant (TCRαc) sequence containing one or more murine regions, a cleavage peptide (e.g., T2A), and a reporter gene. 
       FIG. 3  depicts an exemplary construct backbone sequence for cloning TCRs into expression systems for therapy development. 
       FIG. 4  depicts an exemplary construct sequence for cloning an identified A*0201_LLASSILCA-specific TCR into expression systems for therapy development. 
       FIG. 5  depicts an exemplary construct sequence for cloning an identified A*0101_EVDPIGHLY-specific TCR into expression systems for therapy development. 
     Nucleotides, Vectors, Host Cells, and Related Methods 
     Also provided are isolated nucleic acids encoding HLA-PEPTIDE ABPs, vectors comprising the nucleic acids, and host cells comprising the vectors and nucleic acids, as well as recombinant techniques for the production of the ABPs. 
     The nucleic acids may be recombinant. The recombinant nucleic acids may be constructed outside living cells by joining natural or synthetic nucleic acid segments to nucleic acid molecules that can replicate in a living cell, or replication products thereof. For purposes herein, the replication can be in vitro replication or in vivo replication. 
     For recombinant production of an ABP, the nucleic acid(s) encoding it may be isolated and inserted into a replicable vector for further cloning (i.e., amplification of the DNA) or expression. In some aspects, the nucleic acid may be produced by homologous recombination, for example as described in U.S. Pat. No. 5,204,244, incorporated by reference in its entirety. 
     Many different vectors are known in the art. The vector components generally include one or more of the following: a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter, and a transcription termination sequence, for example as described in U.S. Pat. No. 5,534,615, incorporated by reference in its entirety. 
     Exemplary vectors or constructs suitable for expressing an ABP, e.g., a TCR, CAR, antibody, or antigen binding fragment thereof, include, e.g., the pUC series (Fermentas Life Sciences), the pBluescript series (Stratagene, LaJolla, Calif.), the pET series (Novagen, Madison, Wis.), the pGEX series (Pharmacia Biotech, Uppsala, Sweden), and the pEX series (Clontech, Palo Alto, Calif.). Bacteriophage vectors, such as AGTIO, AGT1 1, AZapII (Stratagene), AEMBL4, and ANM1 149, are also suitable for expressing an ABP disclosed herein. 
     Illustrative examples of suitable host cells are provided below. These host cells are not meant to be limiting, and any suitable host cell may be used to produce the ABPs provided herein. 
     Suitable host cells include any prokaryotic (e.g., bacterial), lower eukaryotic (e.g., yeast), or higher eukaryotic (e.g., mammalian) cells. Suitable prokaryotes include eubacteria, such as Gram-negative or Gram-positive organisms, for example, Enterobacteriaceae such as  Escherichia  ( E. coli ),  Enterobacter, Erwinia, Klebsiella, Proteus, Salmonella  ( S. typhimurium ),  Serratia  ( S. marcescans ),  Shigella , Bacilli ( B. subtilis  and  B. lichenformis ),  Pseudomonas  ( P. aeruginosa ), and  Streptomyces . One useful  E. coli  cloning host is  E. coli  294, although other strains such as  E. coli  B,  E. coli  X1776, and  E. coli  W3110 are also suitable. 
     In addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast are also suitable cloning or expression hosts for HLA-PEPTIDE ABP-encoding vectors.  Saccharomyces cerevisiae , or common baker&#39;s yeast, is a commonly used lower eukaryotic host microorganism. However, a number of other genera, species, and strains are available and useful, such as  Schizosaccharomyces pombe, Kluyveromyces  ( K. lactis, K. fragilis, K. bulgaricus K. wickeramii, K. waltii, K. drosophilarum, K. thermotolerans , and  K. marxianus ),  Yarrowia, Pichia pastoris, Candida  ( C. albicans ),  Trichoderma reesia, Neurospora crassa, Schwanniomyces  ( S. occidentalis ), and filamentous fungi such as, for example  Penicillium, Tolypocladium , and  Aspergillus  ( A. nidulans  and  A. niger ). 
     Useful mammalian host cells include COS-7 cells, HEK293 cells; baby hamster kidney (BHK) cells; Chinese hamster ovary (CHO); mouse sertoli cells; African green monkey kidney cells (VERO-76), and the like. 
     The host cells used to produce the HLA-PEPTIDE ABP may be cultured in a variety of media. Commercially available media such as, for example, Ham&#39;s F10, Minimal Essential Medium (MEM), RPMI-1640, and Dulbecco&#39;s Modified Eagle&#39;s Medium (DMEM) are suitable for culturing the host cells. In addition, any of the media described in Ham et al.,  Meth. Enz.,  1979, 58:44; Barnes et al.,  Anal. Biochem.,  1980, 102:255; and U.S. Pat. Nos. 4,767,704, 4,657,866, 4,927,762, 4,560,655, and 5,122,469; or WO 90/03430 and WO 87/00195 may be used. Each of the foregoing references is incorporated by reference in its entirety. 
     Any of these media may be supplemented as necessary with hormones and/or other growth factors (such as insulin, transferrin, or epidermal growth factor), salts (such as sodium chloride, calcium, magnesium, and phosphate), buffers (such as HEPES), nucleotides (such as adenosine and thymidine), antibiotics, trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range), and glucose or an equivalent energy source. Any other necessary supplements may also be included at appropriate concentrations that would be known to those skilled in the art. 
     The culture conditions, such as temperature, pH, and the like, are those previously used with the host cell selected for expression, and will be apparent to the ordinarily skilled artisan. 
     When using recombinant techniques, the ABP can be produced intracellularly, in the periplasmic space, or directly secreted into the medium. If the ABP is produced intracellularly, as a first step, the particulate debris, either host cells or lysed fragments, is removed, for example, by centrifugation or ultrafiltration. For example, Carter et al. ( Bio/Technology,  1992, 10:163-167, incorporated by reference in its entirety) describes a procedure for isolating ABPs which are secreted to the periplasmic space of  E. coli . Briefly, cell paste is thawed in the presence of sodium acetate (pH 3.5), EDTA, and phenylmethylsulfonylfluoride (PMSF) over about 30 min. Cell debris can be removed by centrifugation. 
     In some embodiments, the ABP is produced in a cell-free system. In some aspects, the cell-free system is an in vitro transcription and translation system as described in Yin et al.,  mAbs,  2012, 4:217-225, incorporated by reference in its entirety. In some aspects, the cell-free system utilizes a cell-free extract from a eukaryotic cell or from a prokaryotic cell. In some aspects, the prokaryotic cell is  E. coli . Cell-free expression of the ABP may be useful, for example, where the ABP accumulates in a cell as an insoluble aggregate, or where yields from periplasmic expression are low. 
     Where the ABP is secreted into the medium, supernatants from such expression systems are generally first concentrated using a commercially available protein concentration filter, for example, an Amicon® or Millipore® Pellcon® ultrafiltration unit. A protease inhibitor such as PMSF may be included in any of the foregoing steps to inhibit proteolysis and antibiotics may be included to prevent the growth of adventitious contaminants. 
     The ABP composition prepared from the cells can be purified using, for example, hydroxylapatite chromatography, gel electrophoresis, dialysis, and affinity chromatography, with affinity chromatography being a particularly useful purification technique. The suitability of protein A as an affinity ligand depends on the species and isotype of any immunoglobulin Fc domain that is present in the ABP. Protein A can be used to purify ABPs that comprise human γ1, γ2, or γ4 heavy chains (Lindmark et al.,  J. Immunol. Meth.,  1983, 62:1-13, incorporated by reference in its entirety). Protein G is useful for all mouse isotypes and for human 73 (Guss et al.,  EMBO J.,  1986, 5:1567-1575, incorporated by reference in its entirety). 
     The matrix to which the affinity ligand is attached is most often agarose, but other matrices are available. Mechanically stable matrices such as controlled pore glass or poly(styrenedivinyl)benzene allow for faster flow rates and shorter processing times than can be achieved with agarose. Where the ABP comprises a CH3 domain, the BakerBond ABX® resin is useful for purification. 
     Other techniques for protein purification, such as fractionation on an ion-exchange column, ethanol precipitation, Reverse Phase HPLC, chromatography on silica, chromatography on heparin Sepharose®, chromatofocusing, SDS-PAGE, and ammonium sulfate precipitation are also available, and can be applied by one of skill in the art. 
     Following any preliminary purification step(s), the mixture comprising the ABP of interest and contaminants may be subjected to low pH hydrophobic interaction chromatography using an elution buffer at a pH between about 2.5 to about 4.5, generally performed at low salt concentrations (e.g., from about 0 to about 0.25 M salt). 
     Methods of Making HLA-PEPTIDE ABPs 
     HLA-PEPTIDE Antigen Preparation 
     The HLA-PEPTIDE antigen used for isolation or creation of the ABPs provided herein may be intact HLA-PEPTIDE or a fragment of HLA-PEPTIDE. The HLA-PEPTIDE antigen may be, for example, in the form of isolated protein or a protein expressed on the surface of a cell. 
     In some embodiments, the HLA-PEPTIDE antigen is a non-naturally occurring variant of HLA-PEPTIDE, such as a HLA-PEPTIDE protein having an amino acid sequence or post-translational modification that does not occur in nature. 
     In some embodiments, the HLA-PEPTIDE antigen is truncated by removal of, for example, intracellular or membrane-spanning sequences, or signal sequences. In some embodiments, the HLA-PEPTIDE antigen is fused at its C-terminus to a human IgG1 Fc domain or a polyhistidine tag. 
     Methods of Identifying ABPs 
     ABPs that bind HLA-PEPTIDE can be identified using any method known in the art, e.g., phage display or immunization of a subject. 
     One method of identifying an antigen binding protein includes providing at least one HLA-PEPTIDE target; and binding the at least one target with an antigen binding protein, thereby identifying the antigen binding protein. The antigen binding protein can be present in a library comprising a plurality of distinct antigen binding proteins. 
     In some embodiments, the library is a phage display library. The phage display library can be developed so that it is substantially free of antigen binding proteins that non-specifically bind the HLA of the HLA-PEPTIDE target. The antigen binding protein can be present in a yeast display library comprising a plurality of distinct antigen binding proteins. The yeast display library can be developed so that it is substantially free of antigen binding proteins that non-specifically bind the HLA of the HLA-PEPTIDE target. 
     In some embodiments, the library is a yeast display library. 
     In some embodiments, the library is a TCR display library. Exemplary TCR display libraries and methods of using such TCR display libraries are described in WO 98/39482; WO 01/62908; WO 2004/044004; WO2005116646, WO2014018863, WO2015136072, WO2017046198; and Helmut et al, (2000) PNAS 97 (26) 14578-14583, which are hereby incorporated by reference in their entirety. 
     In some aspects, the binding step is performed more than once, optionally at least three times, e.g., at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10×. 
     In addition, the method can also include contacting the antigen binding protein with one or more peptide-HLA complexes that are distinct from the HLA-PEPTIDE target to determine if the antigen binding protein selectively binds the HLA-PEPTIDE target. 
     Another method of identifying an antigen binding protein can include obtaining at least one HLA-PEPTIDE target; administering the HLA-PEPTIDE target to a subject (e.g., a mouse, rabbit or a llama), optionally in combination with an adjuvant; and isolating the antigen binding protein from the subject. Isolating the antigen binding protein can include screening the serum of the subject to identify the antigen binding protein. The method can also include contacting the antigen binding protein with one or more peptide-HLA complexes that are distinct from the HLA-PEPTIDE target, e.g., to determine if the antigen binding protein selectively binds to the HLA-PEPTIDE target. An antigen binding protein that is identified can be humanized. 
     In some aspects, isolating the antigen binding protein comprises isolating a B cell from the subject that expresses the antigen binding protein. The B cell can be used to create a hybridoma. The B cell can also be used for cloning one or more of its CDRs. The B cell can also be immortalized, for example, by using EBV transformation. Sequences encoding an antigen binding protein can be cloned from immortalized B cells or can be cloned directly from B cells isolated from an immunized subject. A library that comprises the antigen binding protein of the B cell can also be created, optionally wherein the library is phage display or yeast display. 
     Another method of identifying an antigen binding protein can include obtaining a cell comprising the antigen binding protein; contacting the cell with an HLA-multimer (e.g., a tetramer) comprising at least one HLA-PEPTIDE target; and identifying the antigen binding protein via binding between the HLA-multimer and the antigen binding protein. 
     The cell can be, e.g., a T cell, optionally a cytotoxic T lymphocyte (CTL), or a natural killer (NK) cell, for example. The method can further include isolating the cell, optionally using flow cytometry, magnetic separation, or single cell separation. The method can further include sequencing the antigen binding protein. 
     Another method of identifying an antigen binding protein can include obtaining one or more cells comprising the antigen binding protein; activating the one or more cells with at least one HLA-PEPTIDE target presented on at least one antigen presenting cell (APC); and identifying the antigen binding protein via selection of one or more cells activated by interaction with at least one HLA-PEPTIDE target. 
     The cell can be, e.g., a T cell, optionally a CTL, or an NK cell, for example. The method can further include isolating the cell, optionally using flow cytometry, magnetic separation, or single cell separation. The method can further include sequencing the antigen binding protein. 
     Methods of Making Monoclonal ABPs 
     Monoclonal ABPs may be obtained, for example, using the hybridoma method first described by Kohler et al., Nature, 1975, 256:495-497 (incorporated by reference in its entirety), and/or by recombinant DNA methods (see e.g., U.S. Pat. No. 4,816,567, incorporated by reference in its entirety). Monoclonal ABPs may also be obtained, for example, using phage or yeast-based libraries. See e.g., U.S. Pat. Nos. 8,258,082 and 8,691,730, each of which is incorporated by reference in its entirety. 
     In the hybridoma method, a mouse or other appropriate host animal is immunized to elicit lymphocytes that produce or are capable of producing ABPs that will specifically bind to the protein used for immunization. Alternatively, lymphocytes may be immunized in vitro. Lymphocytes are then fused with myeloma cells using a suitable fusing agent, such as polyethylene glycol, to form a hybridoma cell. See Goding J. W.,  Monoclonal ABPs: Principles and Practice  3 rd  ed. (1986) Academic Press, San Diego, Calif., incorporated by reference in its entirety. 
     The hybridoma cells are seeded and grown in a suitable culture medium that contains one or more substances that inhibit the growth or survival of the unfused, parental myeloma cells. For example, if the parental myeloma cells lack the enzyme hypoxanthine guanine phosphoribosyl transferase (HGPRT or HPRT), the culture medium for the hybridomas typically will include hypoxanthine, aminopterin, and thymidine (HAT medium), which substances prevent the growth of HGPRT-deficient cells. 
     Useful myeloma cells are those that fuse efficiently, support stable high-level production of ABP by the selected ABP-producing cells, and are sensitive media conditions, such as the presence or absence of HAT medium. Among these, preferred myeloma cell lines are murine myeloma lines, such as those derived from MOPC-21 and MC-11 mouse tumors (available from the Salk Institute Cell Distribution Center, San Diego, Calif.), and SP-2 or X63-Ag8-653 cells (available from the American Type Culture Collection, Rockville, Md.). Human myeloma and mouse-human heteromyeloma cell lines also have been described for the production of human monoclonal ABPs. See e.g., Kozbor,  J. Immunol.,  1984, 133:3001, incorporated by reference in its entirety. 
     After the identification of hybridoma cells that produce ABPs of the desired specificity, affinity, and/or biological activity, selected clones may be subcloned by limiting dilution procedures and grown by standard methods. See Goding, supra. Suitable culture media for this purpose include, for example, D-MEM or RPMI-1640 medium. In addition, the hybridoma cells may be grown in vivo as ascites tumors in an animal. 
     DNA encoding the monoclonal ABPs may be readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains of the monoclonal ABPs). Thus, the hybridoma cells can serve as a useful source of DNA encoding ABPs with the desired properties. Once isolated, the DNA may be placed into expression vectors, which are then transfected into host cells such as bacteria (e.g.,  E. coli ), yeast (e.g.,  Saccharomyces  or  Pichia  sp.), COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce ABP, to produce the monoclonal ABPs. 
     Methods of Making Chimeric ABPs 
     Illustrative methods of making chimeric ABPs are described, for example, in U.S. Pat. No. 4,816,567; and Morrison et al.,  Proc. Natl. Acad. Sci. USA,  1984, 81:6851-6855; each of which is incorporated by reference in its entirety. In some embodiments, a chimeric ABP is made by using recombinant techniques to combine a non-human variable region (e.g., a variable region derived from a mouse, rat, hamster, rabbit, or non-human primate, such as a monkey) with a human constant region. 
     Methods of Making Humanized ABPs 
     Humanized ABPs may be generated by replacing most, or all, of the structural portions of a non-human monoclonal ABP with corresponding human ABP sequences. Consequently, a hybrid molecule is generated in which only the antigen-specific variable, or CDR, is composed of non-human sequence. Methods to obtain humanized ABPs include those described in, for example, Winter and Milstein,  Nature,  1991, 349:293-299; Rader et al.,  Proc. Nat. Acad. Sci. U.S.A.,  1998, 95:8910-8915; Steinberger et al.,  J. Biol. Chem.,  2000, 275:36073-36078; Queen et al.,  Proc. Natl. Acad. Sci. U.S.A.,  1989, 86:10029-10033; and U.S. Pat. Nos. 5,585,089, 5,693,761, 5,693,762, and 6,180,370; each of which is incorporated by reference in its entirety. 
     Methods of Making Human ABPs 
     Human ABPs can be generated by a variety of techniques known in the art, for example by using transgenic animals (e.g., humanized mice). See, e.g., Jakobovits et al.,  Proc. Natl. Acad. Sci. U.S.A.,  1993, 90:2551; Jakobovits et al.,  Nature,  1993, 362:255-258; Bruggermann et al.,  Year in Immuno.,  1993, 7:33; and U.S. Pat. Nos. 5,591,669, 5,589,369 and 5,545,807; each of which is incorporated by reference in its entirety. Human ABPs can also be derived from phage-display libraries (see e.g., Hoogenboom et al.,  J. Mol. Biol.,  1991, 227:381-388; Marks et al.,  J. Mol. Biol.,  1991, 222:581-597; and U.S. Pat. Nos. 5,565,332 and 5,573,905; each of which is incorporated by reference in its entirety). Human ABPs may also be generated by in vitro activated B cells (see e.g., U.S. Pat. Nos. 5,567,610 and 5,229,275, each of which is incorporated by reference in its entirety). Human ABPs may also be derived from yeast-based libraries (see e.g., U.S. Pat. No. 8,691,730, incorporated by reference in its entirety). 
     Methods of Making ABP Fragments 
     The ABP fragments provided herein may be made by any suitable method, including the illustrative methods described herein or those known in the art. Suitable methods include recombinant techniques and proteolytic digestion of whole ABPs. Illustrative methods of making ABP fragments are described, for example, in Hudson et al.,  Nat. Med.,  2003, 9:129-134, incorporated by reference in its entirety. Methods of making scFv ABPs are described, for example, in Plückthun, in The Pharmacology of Monoclonal ABPs, vol. 113, Rosenburg and Moore eds., Springer-Verlag, New York, pp. 269-315 (1994); WO 93/16185; and U.S. Pat. Nos. 5,571,894 and 5,587,458; each of which is incorporated by reference in its entirety. 
     Methods of Making Alternative Scaffolds 
     The alternative scaffolds provided herein may be made by any suitable method, including the illustrative methods described herein or those known in the art. For example, methods of preparing Adnectins™ are described in Emanuel et al.,  mAbs,  2011, 3:38-48, incorporated by reference in its entirety. Methods of preparing iMabs are described in U.S. Pat. Pub. No. 2003/0215914, incorporated by reference in its entirety. Methods of preparing Anticalins® are described in Vogt and Skerra, Chem.  Biochem.,  2004, 5:191-199, incorporated by reference in its entirety. Methods of preparing Kunitz domains are described in Wagner et al.,  Biochem . &amp;  Biophys. Res. Comm.,  1992, 186:118-1145, incorporated by reference in its entirety. Methods of preparing thioredoxin peptide aptamers are provided in Geyer and Brent,  Meth. Enzymol.,  2000, 328:171-208, incorporated by reference in its entirety. Methods of preparing Affibodies are provided in Fernandez,  Curr. Opinion in Biotech.,  2004, 15:364-373, incorporated by reference in its entirety. Methods of preparing DARPins are provided in Zahnd et al.,  J. Mol. Biol.,  2007, 369:1015-1028, incorporated by reference in its entirety. Methods of preparing Affilins are provided in Ebersbach et al.,  J. Mol. Biol.,  2007, 372:172-185, incorporated by reference in its entirety. Methods of preparing Tetranectins are provided in Graversen et al.,  J. Biol. Chem.,  2000, 275:37390-37396, incorporated by reference in its entirety. Methods of preparing Avimers are provided in Silverman et al.,  Nature Biotech.,  2005, 23:1556-1561, incorporated by reference in its entirety. Methods of preparing Fynomers are provided in Silacci et al.,  J Biol. Chem.,  2014, 289:14392-14398, incorporated by reference in its entirety. Further information on alternative scaffolds is provided in Binz et al.,  Nat. Biotechnol.,  2005 23:1257-1268; and Skerra,  Current Opin. in Biotech.,  2007 18:295-304, each of which is incorporated by reference in its entirety. 
     Methods of Making Multispecific ABPs 
     The multispecific ABPs provided herein may be made by any suitable method, including the illustrative methods described herein or those known in the art. Methods of making common light chain ABPs are described in Merchant et al.,  Nature Biotechnol.,  1998, 16:677-681, incorporated by reference in its entirety. Methods of making tetravalent bispecific ABPs are described in Coloma and Morrison, Nature  Biotechnol.,  1997, 15:159-163, incorporated by reference in its entirety. Methods of making hybrid immunoglobulins are described in Milstein and Cuello,  Nature,  1983, 305:537-540; and Staerz and Bevan,  Proc. Natl. Acad. Sci. USA,  1986, 83:1453-1457; each of which is incorporated by reference in its entirety. Methods of making immunoglobulins with knobs-into-holes modification are described in U.S. Pat. No. 5,731,168, incorporated by reference in its entirety. Methods of making immunoglobulins with electrostatic modifications are provided in WO 2009/089004, incorporated by reference in its entirety. Methods of making bispecific single chain ABPs are described in Traunecker et al.,  EMBO J.,  1991, 10:3655-3659; and Gruber et al.,  J. Immunol.,  1994, 152:5368-5374; each of which is incorporated by reference in its entirety. Methods of making single-chain ABPs, whose linker length may be varied, are described in U.S. Pat. Nos. 4,946,778 and 5,132,405, each of which is incorporated by reference in its entirety. Methods of making diabodies are described in Hollinger et al.,  Proc. Natl. Acad. Sci. USA,  1993, 90:6444-6448, incorporated by reference in its entirety. Methods of making triabodies and tetrabodies are described in Todorovska et al.,  J. Immunol. Methods,  2001, 248:47-66, incorporated by reference in its entirety. Methods of making trispecific F(ab′)3 derivatives are described in Tutt et al.  J. Immunol.,  1991, 147:60-69, incorporated by reference in its entirety. Methods of making cross-linked ABPs are described in U.S. Pat. No. 4,676,980; Brennan et al.,  Science,  1985, 229:81-83; Staerz, et al.  Nature,  1985, 314:628-631; and EP 0453082; each of which is incorporated by reference in its entirety. Methods of making antigen-binding domains assembled by leucine zippers are described in Kostelny et al.,  J. Immunol.,  1992, 148:1547-1553, incorporated by reference in its entirety. Methods of making ABPs via the DNL approach are described in U.S. Pat. Nos. 7,521,056; 7,550,143; 7,534,866; and 7,527,787; each of which is incorporated by reference in its entirety. Methods of making hybrids of ABP and non-ABP molecules are described in WO 93/08829, incorporated by reference in its entirety, for examples of such ABPs. Methods of making DAF ABPs are described in U.S. Pat. Pub. No. 2008/0069820, incorporated by reference in its entirety. Methods of making ABPs via reduction and oxidation are described in Carlring et al.,  PLoS One,  2011, 6:e22533, incorporated by reference in its entirety. Methods of making DVD-Igs&#39; are described in U.S. Pat. No. 7,612,181, incorporated by reference in its entirety. Methods of making DARTs™ are described in Moore et al.,  Blood,  2011, 117:454-451, incorporated by reference in its entirety. Methods of making DuoBodies® are described in Labrijn et al.,  Proc. Natd. Acad. Sci. USA,  2013, 110:5145-5150; Gramer et al., mAbs, 2013, 5:962-972; and Labrijn et al.,  Nature Protocols,  2014, 9:2450-2463; each of which is incorporated by reference in its entirety. Methods of making ABPs comprising scFvs fused to the C-terminus of the CH3 from an IgG are described in Coloma and Morrison, Nature  Biotechnol.,  1997, 15:159-163, incorporated by reference in its entirety. Methods of making ABPs in which a Fab molecule is attached to the constant region of an immunoglobulin are described in Miler et al.,  J. Immunol.,  2003, 170:4854-4861, incorporated by reference in its entirety. Methods of making CovX-Bodies are described in Doppalapudi et al.,  Proc. Natl. Acad. Sci. USA,  2010, 107:22611-22616, incorporated by reference in its entirety. Methods of making Fcab ABPs are described in Wozniak-Knopp et al.,  Protein Eng. Des. Sel.,  2010, 23:289-297, incorporated by reference in its entirety. Methods of making TandAb® ABPs are described in Kipriyanov et al.,  J. Mol. Biol.,  1999, 293:41-56 and Zhukovsky et al.,  Blood,  2013, 122:5116, each of which is incorporated by reference in its entirety. Methods of making tandem Fabs are described in WO 2015/103072, incorporated by reference in its entirety. Methods of making Zybodies&#39; are described in LaFleur et al.,  mAbs,  2013, 5:208-218, incorporated by reference in its entirety. 
     Methods of Making Variants 
     Any suitable method can be used to introduce variability into a polynucleotide sequence(s) encoding an ABP, including error-prone PCR, chain shuffling, and oligonucleotide-directed mutagenesis such as trinucleotide-directed mutagenesis (TRIM). In some aspects, several CDR residues (e.g., 4-6 residues at a time) are randomized. CDR residues involved in antigen binding may be specifically identified, for example, using alanine scanning mutagenesis or modeling. CDR-H3 and CDR-L3 in particular are often targeted for mutation. 
     The introduction of diversity into the variable regions and/or CDRs can be used to produce a secondary library. The secondary library is then screened to identify ABP variants with improved affinity. Affinity maturation by constructing and reselecting from secondary libraries has been described, for example, in Hoogenboom et al.,  Methods in Molecular Biology,  2001, 178:1-37, incorporated by reference in its entirety. 
     Methods for Engineering Cells with ABPs 
     Also provided are methods, nucleic acids, compositions, and kits, for expressing the ABPs, including receptors comprising antibodies, CARs, and TCRs, and for producing genetically engineered cells expressing such ABPs. The genetic engineering generally involves introduction of a nucleic acid encoding the recombinant or engineered component into the cell, such as by retroviral transduction, transfection, or transformation. 
     In some embodiments, gene transfer is accomplished by first stimulating the cell, such as by combining it with a stimulus that induces a response such as proliferation, survival, and/or activation, e.g., as measured by expression of a cytokine or activation marker, followed by transduction of the activated cells, and expansion in culture to numbers sufficient for clinical applications. 
     In some contexts, overexpression of a stimulatory factor (for example, a lymphokine or a cytokine) may be toxic to a subject. Thus, in some contexts, the engineered cells include gene segments that cause the cells to be susceptible to negative selection in vivo, such as upon administration in adoptive immunotherapy. For example in some aspects, the cells are engineered so that they can be eliminated as a result of a change in the in vivo condition of the patient to which they are administered. The negative selectable phenotype may result from the insertion of a gene that confers sensitivity to an administered agent, for example, a compound. Negative selectable genes include the Herpes simplex virus type I thymidine kinase (HSV-I TK) gene (Wigler et al., Cell II: 223, 1977) which confers ganciclovir sensitivity; the cellular hypoxanthine phosphribosyltransferase (HPRT) gene, the cellular adenine phosphoribosyltransferase (APRT) gene, bacterial cytosine deaminase, (Mullen et al., Proc. Natl. Acad. Sci. USA. 89:33 (1992)). 
     In some aspects, the cells further are engineered to promote expression of cytokines or other factors. Various methods for the introduction of genetically engineered components, e.g., antigen receptors, e.g., CARs, are well known and may be used with the provided methods and compositions. Exemplary methods include those for transfer of nucleic acids encoding the receptors, including via viral, e.g., retroviral or lentiviral, transduction, transposons, and electroporation. 
     In some embodiments, recombinant nucleic acids are transferred into cells using recombinant infectious virus particles, such as, e.g., vectors derived from simian virus 40 (SV40), adenoviruses, adeno-associated virus (AAV). In some embodiments, recombinant nucleic acids are transferred into T cells using recombinant lentiviral vectors or retroviral vectors, such as gamma-retroviral vectors (see, e.g., Koste et al. (2014) Gene Therapy 2014 Apr. 3. doi: 10.1038/gt.2014.25; Carlens et al. (2000) Exp Hematol 28(10): 1137-46; Alonso-Camino et al. (2013) Mol Ther Nucl Acids 2, e93; Park et al., Trends Biotechnol. 2011 Nov. 29(11): 550-557. 
     In some embodiments, the retroviral vector has a long terminal repeat sequence (LTR), e.g., a retroviral vector derived from the Moloney murine leukemia virus (MoMLV), myeloproliferative sarcoma virus (MPSV), murine embryonic stem cell virus (MESV), murine stem cell virus (MSCV), spleen focus forming virus (SFFV), or adeno-associated virus (AAV). Most retroviral vectors are derived from murine retroviruses. In some embodiments, the retroviruses include those derived from any avian or mammalian cell source. The retroviruses typically are amphotropic, meaning that they are capable of infecting host cells of several species, including humans. In one embodiment, the gene to be expressed replaces the retroviral gag, pol and/or env sequences. A number of illustrative retroviral systems have been described (e.g., U.S. Pat. Nos. 5,219,740; 6,207,453; 5,219,740; Miller and Rosman (1989) BioTechniques 7:980-990; Miller, A. D. (1990) Human Gene Therapy 1:5-14; Scarpa et al. (1991) Virology 180:849-852; Burns et al. (1993) Proc. Natl. Acad. Sci. USA 90:8033-8037; and Boris-Lawrie and Temin (1993) Cur. Opin. Genet. Develop. 3:102-109. 
     Methods of lentiviral transduction are known. Exemplary methods are described in, e.g., Wang et al. (2012) J. Immunother. 35(9): 689-701; Cooper et al. (2003) Blood. 101:1637-1644; Verhoeyen et al. (2009) Methods Mol Biol. 506: 97-114; and Cavalieri et al. (2003) Blood. 102(2): 497-505. 
     In some embodiments, recombinant nucleic acids are transferred into T cells via electroporation (see, e.g., Chicaybam et al, (2013) PLoS ONE 8(3): e60298; Van Tedeloo et al. (2000) Gene Therapy 7(16): 1431-1437; and Roth et al. (2018) Nature 559:405-409). In some embodiments, recombinant nucleic acids are transferred into T cells via transposition (see, e.g., Manuri et al. (2010) Hum Gene Ther 21(4): 427-437; Sharma et al. (2013) Molec Ther Nucl Acids 2, e74; and Huang et al. (2009) Methods Mol Biol 506: 115-126). Other methods of introducing and expressing genetic material in immune cells include calcium phosphate transfection (e.g., as described in Current Protocols in Molecular Biology, John Wiley &amp; Sons, New York. N.Y.), protoplast fusion, cationic liposome-mediated transfection; tungsten particle-facilitated microparticle bombardment (Johnston, Nature, 346: 776-777 (1990)); and strontium phosphate DNA co-precipitation (Brash et al., Mol. Cell Biol., 7: 2031-2034 (1987)). 
     Other approaches and vectors for transfer of the nucleic acids encoding the recombinant products are those described, e.g., in international patent application, Publication No.: WO2014055668, and U.S. Pat. No. 7,446,190. 
     Among additional nucleic acids, e.g., genes for introduction are those to improve the efficacy of therapy, such as by promoting viability and/or function of transferred cells; genes to provide a genetic marker for selection and/or evaluation of the cells, such as to assess in vivo survival or localization; genes to improve safety, for example, by making the cell susceptible to negative selection in vivo as described by Lupton S. D. et al., Mol. and Cell Biol., 11:6 (1991); and Riddell et al., Human Gene Therapy 3:319-338 (1992); see also the publications of PCT/US91/08442 and PCT/US94/05601 by Lupton et al. describing the use of bifunctional selectable fusion genes derived from fusing a dominant positive selectable marker with a negative selectable marker. See, e.g., Riddell et al., U.S. Pat. No. 6,040,177, at columns 14-17. 
     Preparation of Engineered Cells 
     In some embodiments, preparation of the engineered cells includes one or more culture and/or preparation steps. The cells for introduction of the HLA-PEPTIDE-ABP, e.g., TCR or CAR, can be isolated from a sample, such as a biological sample, e.g., one obtained from or derived from a subject. In some embodiments, the subject from which the cell is isolated is one having the disease or condition or in need of a cell therapy or to which cell therapy will be administered. The subject in some embodiments is a human in need of a particular therapeutic intervention, such as the adoptive cell therapy for which cells are being isolated, processed, and/or engineered. 
     Accordingly, the cells in some embodiments are primary cells, e.g., primary human cells. The samples include tissue, fluid, and other samples taken directly from the subject, as well as samples resulting from one or more processing steps, such as separation, centrifugation, genetic engineering (e.g. transduction with viral vector), washing, and/or incubation. The biological sample can be a sample obtained directly from a biological source or a sample that is processed. Biological samples include, but are not limited to, body fluids, such as blood, plasma, serum, cerebrospinal fluid, synovial fluid, urine and sweat, tissue and organ samples, including processed samples derived therefrom. 
     In some aspects, the sample from which the cells are derived or isolated is blood or a blood-derived sample, or is or is derived from an apheresis or leukapheresis product. Exemplary samples include whole blood, peripheral blood mononuclear cells (PBMCs), leukocytes, bone marrow, thymus, tissue biopsy, tumor, leukemia, lymphoma, lymph node, gut associated lymphoid tissue, mucosa associated lymphoid tissue, spleen, other lymphoid tissues, liver, lung, stomach, intestine, colon, kidney, pancreas, breast, bone, prostate, cervix, testes, ovaries, tonsil, or other organ, and/or cells derived therefrom. Samples include, in the context of cell therapy, e.g., adoptive cell therapy, samples from autologous and allogeneic sources. 
     In some embodiments, the cells are derived from cell lines, e.g., T cell lines. The cells in some embodiments are obtained from a xenogeneic source, for example, from mouse, rat, non-human primate, or pig. 
     In some embodiments, isolation of the cells includes one or more preparation and/or non-affinity based cell separation steps. In some examples, cells are washed, centrifuged, and/or incubated in the presence of one or more reagents, for example, to remove unwanted components, enrich for desired components, lyse or remove cells sensitive to particular reagents. In some examples, cells are separated based on one or more property, such as density, adherent properties, size, sensitivity and/or resistance to particular components. 
     In some examples, cells from the circulating blood of a subject are obtained, e.g., by apheresis or leukapheresis. The samples, in some aspects, contain lymphocytes, including T cells, monocytes, granulocytes, B cells, other nucleated white blood cells, red blood cells, and/or platelets, and in some aspects contains cells other than red blood cells and platelets. 
     In some embodiments, the blood cells collected from the subject are washed, e.g., to remove the plasma fraction and to place the cells in an appropriate buffer or media for subsequent processing steps. In some embodiments, the cells are washed with phosphate buffered saline (PBS). In some embodiments, the wash solution lacks calcium and/or magnesium and/or many or all divalent cations. In some aspects, a washing step is accomplished a semi-automated “flow-through” centrifuge (for example, the Cobe 2991 cell processor, Baxter) according to the manufacturer&#39;s instructions. In some aspects, a washing step is accomplished by tangential flow filtration (TFF) according to the manufacturer&#39;s instructions. In some embodiments, the cells are resuspended in a variety of biocompatible buffers after washing, such as, for example, Ca++/Mg++ free PBS. In certain embodiments, components of a blood cell sample are removed and the cells directly resuspended in culture media. 
     In some embodiments, the methods include density-based cell separation methods, such as the preparation of white blood cells from peripheral blood by lysing the red blood cells and centrifugation through a Percoll or Ficoll gradient. 
     In some embodiments, the isolation methods include the separation of different cell types based on the expression or presence in the cell of one or more specific molecules, such as surface markers, e.g., surface proteins, intracellular markers, or nucleic acid. In some embodiments, any known method for separation based on such markers may be used. In some embodiments, the separation is affinity- or immunoaffinity-based separation. For example, the isolation in some aspects includes separation of cells and cell populations based on the cells&#39; expression or expression level of one or more markers, typically cell surface markers, for example, by incubation with an antibody or binding partner that specifically binds to such markers, followed generally by washing steps and separation of cells having bound the antibody or binding partner, from those cells having not bound to the antibody or binding partner. 
     Such separation steps can be based on positive selection, in which the cells having bound the reagents are retained for further use, and/or negative selection, in which the cells having not bound to the antibody or binding partner are retained. In some examples, both fractions are retained for further use. In some aspects, negative selection can be particularly useful where no antibody is available that specifically identifies a cell type in a heterogeneous population, such that separation is best carried out based on markers expressed by cells other than the desired population. 
     The separation need not result in 100% enrichment or removal of a particular cell population or cells expressing a particular marker. For example, positive selection of or enrichment for cells of a particular type, such as those expressing a marker, refers to increasing the number or percentage of such cells, but need not result in a complete absence of cells not expressing the marker. Likewise, negative selection, removal, or depletion of cells of a particular type, such as those expressing a marker, refers to decreasing the number or percentage of such cells, but need not result in a complete removal of all such cells. 
     In some examples, multiple rounds of separation steps are carried out, where the positively or negatively selected fraction from one step is subjected to another separation step, such as a subsequent positive or negative selection. In some examples, a single separation step can deplete cells expressing multiple markers simultaneously, such as by incubating cells with a plurality of antibodies or binding partners, each specific for a marker targeted for negative selection. Likewise, multiple cell types can simultaneously be positively selected by incubating cells with a plurality of antibodies or binding partners expressed on the various cell types. 
     For example, in some aspects, specific subpopulations of T cells, such as cells positive or expressing high levels of one or more surface markers, e.g., CD28+, CD62L+, CCR7+, CD27+, CD127+, CD4+, CD8+, CD45RA+, and/or CD45RO+ T cells, are isolated by positive or negative selection techniques. 
     For example, CD3+, CD28+ T cells can be positively selected using CD3/CD28 conjugated magnetic beads (e.g., DYNABEADS® M-450 CD3/CD28 T Cell Expander). 
     In some embodiments, isolation is carried out by enrichment for a particular cell population by positive selection, or depletion of a particular cell population, by negative selection. In some embodiments, positive or negative selection is accomplished by incubating cells with one or more antibodies or other binding agent that specifically bind to one or more surface markers expressed or expressed (marker+) at a relatively higher level (marker high ) on the positively or negatively selected cells, respectively. 
     In some embodiments, T cells are separated from a peripheral blood mononuclear cell (PBMC) sample by negative selection of markers expressed on non-T cells, such as B cells, monocytes, or other white blood cells, such as CD14. In some aspects, a CD4+ or CD8+ selection step is used to separate CD4+ helper and CD8+ cytotoxic T cells. Such CD4+ and CD8+ populations can be further sorted into sub-populations by positive or negative selection for markers expressed or expressed to a relatively higher degree on one or more naive, memory, and/or effector T cell subpopulations. 
     In some embodiments, CD8+ cells are further enriched for or depleted of naive, central memory, effector memory, and/or central memory stem cells, such as by positive or negative selection based on surface antigens associated with the respective subpopulation. In some embodiments, enrichment for central memory T (TCM) cells is carried out to increase efficacy, such as to improve long-term survival, expansion, and/or engraftment following administration, which in some aspects is particularly robust in such sub-populations. See Terakura et al. (2012) Blood. 1:72-82; Wang et al. (2012) J Immunother. 35(9):689-701. In some embodiments, combining TCM-enriched CD8+ T cells and CD4+ T cells further enhances efficacy. 
     In embodiments, memory T cells are present in both CD62L+ and CD62L− subsets of CD8+ peripheral blood lymphocytes. Peripheral blood mononuclear cell (PBMC) can be enriched for or depleted of CD62L-CD8+ and/or CD62L+CD8+ fractions, such as using anti-CD8 and anti-CD62L antibodies. 
     In some embodiments, the enrichment for central memory T (TCM) cells is based on positive or high surface expression of CD45RO, CD62L, CCR7, CD28, CD3, and/or CD 127; in some aspects, it is based on negative selection for cells expressing or highly expressing CD45RA and/or granzyme B. In some aspects, isolation of a CD8+ population enriched for TCM cells is carried out by depletion of cells expressing CD4, CD14, CD45RA, and positive selection or enrichment for cells expressing CD62L. In one aspect, enrichment for central memory T (TCM) cells is carried out starting with a negative fraction of cells selected based on CD4 expression, which is subjected to a negative selection based on expression of CD14 and CD45RA, and a positive selection based on CD62L. Such selections in some aspects are carried out simultaneously and in other aspects are carried out sequentially, in either order. In some aspects, the same CD4 expression-based selection step used in preparing the CD8+ cell population or subpopulation, also is used to generate the CD4+ cell population or sub-population, such that both the positive and negative fractions from the CD4-based separation are retained and used in subsequent steps of the methods, optionally following one or more further positive or negative selection steps. 
     In a particular example, a sample of PBMCs or other white blood cell sample is subjected to selection of CD4+ cells, where both the negative and positive fractions are retained. The negative fraction then is subjected to negative selection based on expression of CD14 and CD45RA or ROR1, and positive selection based on a marker characteristic of central memory T cells, such as CD62L or CCR7, where the positive and negative selections are carried out in either order. 
     CD4+ T helper cells are sorted into naive, central memory, and effector cells by identifying cell populations that have cell surface antigens. CD4+ lymphocytes can be obtained by standard methods. In some embodiments, naive CD4+ T lymphocytes are CD45RO−, CD45RA+, CD62L+, CD4+ T cells. In some embodiments, central memory CD4+ cells are CD62L+ and CD45RO+. In some embodiments, effector CD4+ cells are CD62L− and CD45RO−. 
     In one example, to enrich for CD4+ cells by negative selection, a monoclonal antibody cocktail typically includes antibodies to CD14, CD20, CD11b, CD16, HLA-DR, and CD8. In some embodiments, the antibody or binding partner is bound to a solid support or matrix, such as a magnetic bead or paramagnetic bead, to allow for separation of cells for positive and/or negative selection. For example, in some embodiments, the cells and cell populations are separated or isolated using immune-magnetic (or affinity-magnetic) separation techniques (reviewed in Methods in Molecular Medicine, vol. 58: Metastasis Research Protocols, Vol. 2: Cell Behavior In Vitro and In Vivo, p 17-25 Edited by: S. A. Brooks and U. Schumacher Humana Press Inc., Totowa, N.J.). 
     In some aspects, the sample or composition of cells to be separated is incubated with small, magnetizable or magnetically responsive material, such as magnetically responsive particles or microparticles, such as paramagnetic beads (e.g., such as Dynabeads or MACS beads). The magnetically responsive material, e.g., particle, generally is directly or indirectly attached to a binding partner, e.g., an antibody, that specifically binds to a molecule, e.g., surface marker, present on the cell, cells, or population of cells that it is desired to separate, e.g., that it is desired to negatively or positively select. 
     In some embodiments, the magnetic particle or bead comprises a magnetically responsive material bound to a specific binding member, such as an antibody or other binding partner. There are many well-known magnetically responsive materials used in magnetic separation methods. Suitable magnetic particles include those described in Molday, U.S. Pat. No. 4,452,773, and in European Patent Specification EP 452342 B, which are hereby incorporated by reference. Colloidal sized particles, such as those described in Owen U.S. Pat. No. 4,795,698, and Liberti et al., U.S. Pat. No. 5,200,084 are other examples. 
     The incubation generally is carried out under conditions whereby the antibodies or binding partners, or molecules, such as secondary antibodies or other reagents, which specifically bind to such antibodies or binding partners, which are attached to the magnetic particle or bead, specifically bind to cell surface molecules if present on cells within the sample. 
     In some aspects, the sample is placed in a magnetic field, and those cells having magnetically responsive or magnetizable particles attached thereto will be attracted to the magnet and separated from the unlabeled cells. For positive selection, cells that are attracted to the magnet are retained; for negative selection, cells that are not attracted (unlabeled cells) are retained. In some aspects, a combination of positive and negative selection is performed during the same selection step, where the positive and negative fractions are retained and further processed or subject to further separation steps. 
     In certain embodiments, the magnetically responsive particles are coated in primary antibodies or other binding partners, secondary antibodies, lectins, enzymes, or streptavidin. In certain embodiments, the magnetic particles are attached to cells via a coating of primary antibodies specific for one or more markers. In certain embodiments, the cells, rather than the beads, are labeled with a primary antibody or binding partner, and then cell-type specific secondary antibody- or other binding partner (e.g., streptavidin)-coated magnetic particles, are added. In certain embodiments, streptavidin-coated magnetic particles are used in conjunction with biotinylated primary or secondary antibodies. 
     In some embodiments, the magnetically responsive particles are left attached to the cells that are to be subsequently incubated, cultured and/or engineered; in some aspects, the particles are left attached to the cells for administration to a patient. In some embodiments, the magnetizable or magnetically responsive particles are removed from the cells. Methods for removing magnetizable particles from cells are known and include, e.g., the use of competing non-labeled antibodies, magnetizable particles or antibodies conjugated to cleavable linkers, etc. In some embodiments, the magnetizable particles are biodegradable. 
     In some embodiments, the affinity-based selection is via magnetic-activated cell sorting (MACS) (Miltenyi Biotech, Auburn, Calif.). Magnetic Activated Cell Sorting (MACS) systems are capable of high-purity selection of cells having magnetized particles attached thereto. In certain embodiments, MACS operates in a mode wherein the non-target and target species are sequentially eluted after the application of the external magnetic field. That is, the cells attached to magnetized particles are held in place while the unattached species are eluted. Then, after this first elution step is completed, the species that were trapped in the magnetic field and were prevented from being eluted are freed in some manner such that they can be eluted and recovered. In certain embodiments, the non-target cells are labelled and depleted from the heterogeneous population of cells. 
     In certain embodiments, the isolation or separation is carried out using a system, device, or apparatus that carries out one or more of the isolation, cell preparation, separation, processing, incubation, culture, and/or formulation steps of the methods. In some aspects, the system is used to carry out each of these steps in a closed or sterile environment, for example, to minimize error, user handling and/or contamination. In one example, the system is a system as described in International Patent Application, Publication Number WO2009/072003, or US 20110003380 A1. 
     In some embodiments, the system or apparatus carries out one or more, e.g., all, of the isolation, processing, engineering, and formulation steps in an integrated or self-contained system, and/or in an automated or programmable fashion. In some aspects, the system or apparatus includes a computer and/or computer program in communication with the system or apparatus, which allows a user to program, control, assess the outcome of, and/or adjust various aspects of the processing, isolation, engineering, and formulation steps. 
     In some aspects, the separation and/or other steps is carried out using CliniMACS system (Miltenyi Biotec), for example, for automated separation of cells on a clinical-scale level in a closed and sterile system. Components can include an integrated microcomputer, magnetic separation unit, peristaltic pump, and various pinch valves. The integrated computer in some aspects controls all components of the instrument and directs the system to perform repeated procedures in a standardized sequence. The magnetic separation unit in some aspects includes a movable permanent magnet and a holder for the selection column. The peristaltic pump controls the flow rate throughout the tubing set and, together with the pinch valves, ensures the controlled flow of buffer through the system and continual suspension of cells. 
     The CliniMACS system in some aspects uses antibody-coupled magnetizable particles that are supplied in a sterile, non-pyrogenic solution. In some embodiments, after labelling of cells with magnetic particles the cells are washed to remove excess particles. A cell preparation bag is then connected to the tubing set, which in turn is connected to a bag containing buffer and a cell collection bag. The tubing set consists of pre-assembled sterile tubing, including a pre-column and a separation column, and are for single use only. After initiation of the separation program, the system automatically applies the cell sample onto the separation column. Labeled cells are retained within the column, while unlabeled cells are removed by a series of washing steps. In some embodiments, the cell populations for use with the methods described herein are unlabeled and are not retained in the column. In some embodiments, the cell populations for use with the methods described herein are labeled and are retained in the column. In some embodiments, the cell populations for use with the methods described herein are eluted from the column after removal of the magnetic field, and are collected within the cell collection bag. 
     In certain embodiments, separation and/or other steps are carried out using the CliniMACS Prodigy system (Miltenyi Biotec). The CliniMACS Prodigy system in some aspects is equipped with a cell processing unity that permits automated washing and fractionation of cells by centrifugation. The CliniMACS Prodigy system can also include an onboard camera and image recognition software that determines the optimal cell fractionation endpoint by discerning the macroscopic layers of the source cell product. For example, peripheral blood may be automatically separated into erythrocytes, white blood cells and plasma layers. The CliniMACS Prodigy system can also include an integrated cell cultivation chamber which accomplishes cell culture protocols such as, e.g., cell differentiation and expansion, antigen loading, and long-term cell culture. Input ports can allow for the sterile removal and replenishment of media and cells can be monitored using an integrated microscope. See, e.g., Klebanoff et al. (2012) J Immunother. 35(9): 651-660, Terakura et al. (2012) Blood. 1:72-82, and Wang et al. (2012) J Immunother. 35(9):689-701. 
     In some embodiments, a cell population described herein is collected and enriched (or depleted) via flow cytometry, in which cells stained for multiple cell surface markers are carried in a fluidic stream. In some embodiments, a cell population described herein is collected and enriched (or depleted) via preparative scale fluorescence activated cell sorting (FACS). In certain embodiments, a cell population described herein is collected and enriched (or depleted) by use of microelectromechanical systems (MEMS) chips in combination with a FACS-based detection system (see, e.g., WO 2010/033140, Cho et al. (2010) Lab Chip 10, 1567-1573; and Godin et al. (2008) J Biophoton. 1(5):355-376. In both cases, cells can be labeled with multiple markers, allowing for the isolation of well-defined T cell subsets at high purity. 
     In some embodiments, the antibodies or binding partners are labeled with one or more detectable marker, to facilitate separation for positive and/or negative selection. For example, separation may be based on binding to fluorescently labeled antibodies. In some examples, separation of cells based on binding of antibodies or other binding partners specific for one or more cell surface markers are carried in a fluidic stream, such as by fluorescence-activated cell sorting (FACS), including preparative scale (FACS) and/or microelectromechanical systems (MEMS) chips, e.g., in combination with a flow-cytometric detection system. Such methods allow for positive and negative selection based on multiple markers simultaneously. 
     In some embodiments, the preparation methods include steps for freezing, e.g., cryopreserving, the cells, either before or after isolation, incubation, and/or engineering. In some embodiments, the freeze and subsequent thaw step removes granulocytes and, to some extent, monocytes in the cell population. In some embodiments, the cells are suspended in a freezing solution, e.g., following a washing step to remove plasma and platelets. Any of a variety of known freezing solutions and parameters in some aspects may be used. One example involves using PBS containing 20% DMSO and 8% human serum albumin (HSA), or other suitable cell freezing media. This can then be diluted 1:1 with media so that the final concentration of DMSO and HSA are 10% and 4%, respectively. Other examples include Cryostor®, CTL-Cryo™ ABC freezing media, and the like. The cells are then frozen to −80 degrees C. at a rate of 1 degree per minute and stored in the vapor phase of a liquid nitrogen storage tank. 
     In some embodiments, the provided methods include cultivation, incubation, culture, and/or genetic engineering steps. For example, in some embodiments, provided are methods for incubating and/or engineering the depleted cell populations and culture-initiating compositions. 
     Thus, in some embodiments, the cell populations are incubated in a culture-initiating composition. The incubation and/or engineering may be carried out in a culture vessel, such as a unit, chamber, well, column, tube, tubing set, valve, vial, culture dish, bag, or other container for culture or cultivating cells. 
     In some embodiments, the cells are incubated and/or cultured prior to or in connection with genetic engineering. The incubation steps can include culture, cultivation, stimulation, activation, and/or propagation. In some embodiments, the compositions or cells are incubated in the presence of stimulating conditions or a stimulatory agent. Such conditions include those designed to induce proliferation, expansion, activation, and/or survival of cells in the population, to mimic antigen exposure, and/or to prime the cells for genetic engineering, such as for the introduction of a recombinant antigen receptor. 
     The conditions can include one or more of particular media, temperature, oxygen content, carbon dioxide content, time, agents, e.g., nutrients, amino acids, antibiotics, ions, and/or stimulatory factors, such as cytokines, chemokines, antigens, binding partners, fusion proteins, recombinant soluble receptors, and any other agents designed to activate the cells. 
     In some embodiments, the stimulating conditions or agents include one or more agent, e.g., ligand, which is capable of activating an intracellular signaling domain of a TCR complex. In some aspects, the agent turns on or initiates TCR/CD3 intracellular signaling cascade in a T cell. Such agents can include antibodies, such as those specific for a TCR component and/or costimulatory receptor, e.g., anti-CD3, anti-CD28, for example, bound to solid support such as a bead, and/or one or more cytokines. Optionally, the expansion method may further comprise the step of adding anti-CD3 and/or anti CD28 antibody to the culture medium (e.g., at a concentration of at least about 0.5 ng/ml). In some embodiments, the stimulating agents include IL-2 and/or IL-15, for example, an IL-2 concentration of at least about 10 units/mL. 
     In some aspects, incubation is carried out in accordance with techniques such as those described in U.S. Pat. No. 6,040,177 to Riddell et al., Klebanoff et al. (2012) J Immunother. 35(9): 651-660, Terakura et al. (2012) Blood. 1:72-82, and/or Wang et al. (2012) J Immunother. 35(9):689-701. 
     In some embodiments, the T cells are expanded by adding to the culture-initiating composition feeder cells, such as non-dividing peripheral blood mononuclear cells (PBMC), (e.g., such that the resulting population of cells contains at least about 5, 10, 20, or 40 or more PBMC feeder cells for each T lymphocyte in the initial population to be expanded); and incubating the culture (e.g. for a time sufficient to expand the numbers of T cells). In some aspects, the non-dividing feeder cells can comprise gamma-irradiated PBMC feeder cells. In some embodiments, the PBMC are irradiated with gamma rays in the range of about 3000 to 3600 rads to prevent cell division. In some embodiments, the PBMC feeder cells are inactivated with Mytomicin C. In some aspects, the feeder cells are added to culture medium prior to the addition of the populations of T cells. 
     In some embodiments, the stimulating conditions include temperature suitable for the growth of human T lymphocytes, for example, at least about 25 degrees Celsius, generally at least about 30 degrees, and generally at or about 37 degrees Celsius. Optionally, the incubation may further comprise adding non-dividing EBV-transformed lymphoblastoid cells (LCL) as feeder cells. LCL can be irradiated with gamma rays in the range of about 6000 to 10,000 rads. The LCL feeder cells in some aspects is provided in any suitable amount, such as a ratio of LCL feeder cells to initial T lymphocytes of at least about 10:1. 
     In embodiments, antigen-specific T cells, such as antigen-specific CD4+ and/or CD8+ T cells, are obtained by stimulating naive or antigen specific T lymphocytes with antigen. For example, antigen-specific T cell lines or clones can be generated to cytomegalovirus antigens by isolating T cells from infected subjects and stimulating the cells in vitro with the same antigen. 
     Assays 
     A variety of assays known in the art may be used to identify and characterize an HLA-PEPTIDE ABP provided herein. 
     Binding, Competition, and Epitope Mapping Assays 
     Specific antigen-binding activity of an ABP provided herein may be evaluated by any suitable method, including using SPR, BLI, RIA and MSD-SET, as described elsewhere in this disclosure. Additionally, antigen-binding activity may be evaluated by ELISA assays, using flow cytometry, and/or Western blot assays. 
     Assays for measuring competition between two ABPs, or an ABP and another molecule (e.g., one or more ligands of HLA-PEPTIDE such as a TCR) are described elsewhere in this disclosure and, for example, in Harlow and Lane,  ABPs: A Laboratory Manual  ch.14, 1988, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., incorporated by reference in its entirety. 
     Assays for mapping the epitopes to which an ABP provided herein bind are described, for example, in Morris “Epitope Mapping Protocols,” in  Methods in Molecular Biology  vol. 66, 1996, Humana Press, Totowa, N.J., incorporated by reference in its entirety. In some embodiments, the epitope is determined by peptide competition. In some embodiments, the epitope is determined by mass spectrometry. In some embodiments, the epitope is determined by mutagenesis. In some embodiments, the epitope is determined by crystallography. 
     Assays for Effector Functions 
     Effector function following treatment with an ABP and/or cell provided herein may be evaluated using a variety of in vitro and in vivo assays known in the art, including those described in Ravetch and Kinet,  Annu. Rev. Immunol.,  1991, 9:457-492; U.S. Pat. Nos. 5,500,362, 5,821,337; Hellstrom et al.,  Proc. Nat&#39;l Acad. Sci. USA,  1986, 83:7059-7063; Hellstrom et al.,  Proc. Nat&#39;l Acad. Sci. USA,  1985, 82:1499-1502; Bruggemann et al.,  J Exp. Med.,  1987, 166:1351-1361; Clynes et al.,  Proc. Nat&#39;l Acad. Sci. USA,  1998, 95:652-656; WO 2006/029879; WO 2005/100402; Gazzano-Santoro et al.,  J. Immunol. Methods,  1996, 202:163-171; Cragg et al.,  Blood,  2003, 101:1045-1052; Cragg et al.  Blood,  2004, 103:2738-2743; and Petkova et al.,  Int&#39;l. Immunol.,  2006, 18:1759-1769; each of which is incorporated by reference in its entirety. 
     Pharmaceutical Compositions 
     An ABP, cell, or HLA-PEPTIDE target provided herein can be formulated in any appropriate pharmaceutical composition and administered by any suitable route of administration. Suitable routes of administration include, but are not limited to, the intra-arterial, intradermal, intramuscular, intraperitoneal, intravenous, nasal, parenteral, pulmonary, and subcutaneous routes. 
     The pharmaceutical composition may comprise one or more pharmaceutical excipients. Any suitable pharmaceutical excipient may be used, and one of ordinary skill in the art is capable of selecting suitable pharmaceutical excipients. Accordingly, the pharmaceutical excipients provided below are intended to be illustrative, and not limiting. Additional pharmaceutical excipients include, for example, those described in the  Handbook of Pharmaceutical Excipients , Rowe et al. (Eds.) 6th Ed. (2009), incorporated by reference in its entirety. 
     In some embodiments, the pharmaceutical composition comprises an anti-foaming agent. Any suitable anti-foaming agent may be used. In some aspects, the anti-foaming agent is selected from an alcohol, an ether, an oil, a wax, a silicone, a surfactant, and combinations thereof. In some aspects, the anti-foaming agent is selected from a mineral oil, a vegetable oil, ethylene bis stearamide, a paraffin wax, an ester wax, a fatty alcohol wax, a long chain fatty alcohol, a fatty acid soap, a fatty acid ester, a silicon glycol, a fluorosilicone, a polyethylene glycol-polypropylene glycol copolymer, polydimethylsiloxane-silicon dioxide, ether, octyl alcohol, capryl alcohol, sorbitan trioleate, ethyl alcohol, 2-ethyl-hexanol, dimethicone, oleyl alcohol, simethicone, and combinations thereof. 
     In some embodiments, the pharmaceutical composition comprises a co-solvent. Illustrative examples of co-solvents include ethanol, poly(ethylene) glycol, butylene glycol, dimethylacetamide, glycerin, propylene glycol, and combinations thereof. 
     In some embodiments, the pharmaceutical composition comprises a buffer. Illustrative examples of buffers include acetate, borate, carbonate, lactate, malate, phosphate, citrate, hydroxide, diethanolamine, monoethanolamine, glycine, methionine, guar gum, monosodium glutamate, and combinations thereof. 
     In some embodiments, the pharmaceutical composition comprises a carrier or filler. Illustrative examples of carriers or fillers include lactose, maltodextrin, mannitol, sorbitol, chitosan, stearic acid, xanthan gum, guar gum, and combinations thereof. 
     In some embodiments, the pharmaceutical composition comprises a surfactant. Illustrative examples of surfactants include d-alpha tocopherol, benzalkonium chloride, benzethonium chloride, cetrimide, cetylpyridinium chloride, docusate sodium, glyceryl behenate, glyceryl monooleate, lauric acid, macrogol 15 hydroxystearate, myristyl alcohol, phospholipids, polyoxyethylene alkyl ethers, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, polyoxylglycerides, sodium lauryl sulfate, sorbitan esters, vitamin E polyethylene(glycol) succinate, and combinations thereof. 
     In some embodiments, the pharmaceutical composition comprises an anti-caking agent. Illustrative examples of anti-caking agents include calcium phosphate (tribasic), hydroxymethyl cellulose, hydroxypropyl cellulose, magnesium oxide, and combinations thereof. 
     Other excipients that may be used with the pharmaceutical compositions include, for example, albumin, antioxidants, antibacterial agents, antifungal agents, bioabsorbable polymers, chelating agents, controlled release agents, diluents, dispersing agents, dissolution enhancers, emulsifying agents, gelling agents, ointment bases, penetration enhancers, preservatives, solubilizing agents, solvents, stabilizing agents, sugars, and combinations thereof. Specific examples of each of these agents are described, for example, in the  Handbook of Pharmaceutical Excipients , Rowe et al. (Eds.) 6th Ed. (2009), The Pharmaceutical Press, incorporated by reference in its entirety. 
     In some embodiments, the pharmaceutical composition comprises a solvent. In some aspects, the solvent is saline solution, such as a sterile isotonic saline solution or dextrose solution. In some aspects, the solvent is water for injection. 
     In some embodiments, the pharmaceutical compositions are in a particulate form, such as a microparticle or a nanoparticle. Microparticles and nanoparticles may be formed from any suitable material, such as a polymer or a lipid. In some aspects, the microparticles or nanoparticles are micelles, liposomes, or polymersomes. 
     Further provided herein are anhydrous pharmaceutical compositions and dosage forms comprising an ABP, since water can facilitate the degradation of some ABPs. 
     Anhydrous pharmaceutical compositions and dosage forms provided herein can be prepared using anhydrous or low moisture containing ingredients and low moisture or low humidity conditions. Pharmaceutical compositions and dosage forms that comprise lactose and at least one active ingredient that comprises a primary or secondary amine can be anhydrous if substantial contact with moisture and/or humidity during manufacturing, packaging, and/or storage is expected. 
     An anhydrous pharmaceutical composition should be prepared and stored such that its anhydrous nature is maintained. Accordingly, anhydrous compositions can be packaged using materials known to prevent exposure to water such that they can be included in suitable formulary kits. Examples of suitable packaging include, but are not limited to, hermetically sealed foils, plastics, unit dose containers (e.g., vials), blister packs, and strip packs. 
     In certain embodiments, an ABP and/or cell provided herein is formulated as parenteral dosage forms. Parenteral dosage forms can be administered to subjects by various routes including, but not limited to, subcutaneous, intravenous (including infusions and bolus injections), intramuscular, and intra-arterial. Because their administration typically bypasses subjects&#39; natural defenses against contaminants, parenteral dosage forms are typically, sterile or capable of being sterilized prior to administration to a subject. Examples of parenteral dosage forms include, but are not limited to, solutions ready for injection, dry (e.g., lyophilized) products ready to be dissolved or suspended in a pharmaceutically acceptable vehicle for injection, suspensions ready for injection, and emulsions. 
     Suitable vehicles that can be used to provide parenteral dosage forms are well known to those skilled in the art. Examples include, but are not limited to: Water for Injection USP; aqueous vehicles such as, but not limited to, Sodium Chloride Injection, Ringer&#39;s Injection, Dextrose Injection, Dextrose and Sodium Chloride Injection, and Lactated Ringer&#39;s Injection; water miscible vehicles such as, but not limited to, ethyl alcohol, polyethylene glycol, and polypropylene glycol; and non-aqueous vehicles such as, but not limited to, corn oil, cottonseed oil, peanut oil, sesame oil, ethyl oleate, isopropyl myristate, and benzyl benzoate. 
     Excipients that increase the solubility of one or more of the ABPs and/or cells disclosed herein can also be incorporated into the parenteral dosage forms. 
     In some embodiments, the parenteral dosage form is lyophilized. Exemplary lyophilized formulations are described, for example, in U.S. Pat. Nos. 6,267,958 and 6,171,586; and WO 2006/044908; each of which is incorporated by reference in its entirety. 
     In human therapeutics, the doctor will determine the posology which he considers most appropriate according to a preventive or curative treatment and according to the age, weight, condition and other factors specific to the subject to be treated. 
     In certain embodiments, a composition provided herein is a pharmaceutical composition or a single unit dosage form. Pharmaceutical compositions and single unit dosage forms provided herein comprise a prophylactically or therapeutically effective amount of one or more prophylactic or therapeutic ABP. 
     The amount of the ABP, cell, or composition which will be effective in the prevention or treatment of a disorder or one or more symptoms thereof will vary with the nature and severity of the disease or condition, and the route by which the ABP and/or cell is administered. The frequency and dosage will also vary according to factors specific for each subject depending on the specific therapy (e.g., therapeutic or prophylactic agents) administered, the severity of the disorder, disease, or condition, the route of administration, as well as age, body, weight, response, and the past medical history of the subject. Effective doses may be extrapolated from dose-response curves derived from in vitro or animal model test systems. 
     Different therapeutically effective amounts may be applicable for different diseases and conditions, as will be readily known by those of ordinary skill in the art. Similarly, amounts sufficient to prevent, manage, treat or ameliorate such disorders, but insufficient to cause, or sufficient to reduce, adverse effects associated with the ABPs and/or cells provided herein are also encompassed by the dosage amounts and dose frequency schedules provided herein. Further, when a subject is administered multiple dosages of a composition provided herein, not all of the dosages need be the same. For example, the dosage administered to the subject may be increased to improve the prophylactic or therapeutic effect of the composition or it may be decreased to reduce one or more side effects that a particular subject is experiencing. 
     In certain embodiments, treatment or prevention can be initiated with one or more loading doses of an ABP or composition provided herein followed by one or more maintenance doses. 
     In certain embodiments, a dose of an ABP, cell, or composition provided herein can be administered to achieve a steady-state concentration of the ABP and/or cell in blood or serum of the subject. The steady-state concentration can be determined by measurement according to techniques available to those of skill or can be based on the physical characteristics of the subject such as height, weight and age. 
     As discussed in more detail elsewhere in this disclosure, an ABP and/or cell provided herein may optionally be administered with one or more additional agents useful to prevent or treat a disease or disorder. The effective amount of such additional agents may depend on the amount of ABP present in the formulation, the type of disorder or treatment, and the other factors known in the art or described herein. 
     Therapeutic Applications 
     For therapeutic applications, ABPs and/or cells are administered to a mammal, generally a human, in a pharmaceutically acceptable dosage form such as those known in the art and those discussed above. For example, ABPs and/or cells may be administered to a human intravenously as a bolus or by continuous infusion over a period of time, by intramuscular, intraperitoneal, intra-cerebrospinal, subcutaneous, intra-articular, intrasynovial, intrathecal, or intratumoral routes. The ABPs also are suitably administered by peritumoral, intralesional, or perilesional routes, to exert local as well as systemic therapeutic effects. The intraperitoneal route may be particularly useful, for example, in the treatment of ovarian tumors. 
     The ABPs and/or cells provided herein can be useful for the treatment of any disease or condition involving HLA-PEPTIDE. In some embodiments, the disease or condition is a disease or condition that can benefit from treatment with an anti-HLA-PEPTIDE ABP and/or cell. In some embodiments, the disease or condition is a tumor. In some embodiments, the disease or condition is a cell proliferative disorder. In some embodiments, the disease or condition is a cancer. 
     In some embodiments, the ABPs and/or cells provided herein are provided for use as a medicament. In some embodiments, the ABPs and/or cells provided herein are provided for use in the manufacture or preparation of a medicament. In some embodiments, the medicament is for the treatment of a disease or condition that can benefit from an anti-HLA-PEPTIDE ABP and/or cell. In some embodiments, the disease or condition is a tumor. In some embodiments, the disease or condition is a cell proliferative disorder. In some embodiments, the disease or condition is a cancer. 
     In some embodiments, provided herein is a method of treating a disease or condition in a subject in need thereof by administering an effective amount of an ABP and/or cell provided herein to the subject. In some aspects, the disease or condition is a cancer. 
     In some embodiments, provided herein is a method of treating a disease or condition in a subject in need thereof by administering an effective amount of an ABP and/or cell provided herein to the subject, wherein the disease or condition is a cancer, and the cancer is selected from a solid tumor and a hematological tumor. 
     In some embodiments, provided herein is a method of modulating an immune response in a subject in need thereof, comprising administering to the subject an effective amount of an ABP and/or cell or a pharmaceutical composition disclosed herein. 
     Combination Therapies 
     In some embodiments, an ABP and/or cell provided herein is administered with at least one additional therapeutic agent. Any suitable additional therapeutic agent may be administered with an ABP and/or cell provided herein. An additional therapeutic agent can be fused to an ABP. In some aspects, the additional therapeutic agent is selected from radiation, a cytotoxic agent, a toxin, a chemotherapeutic agent, a cytostatic agent, an anti-hormonal agent, an EGFR inhibitor, an immunomodulatory agent, an anti-angiogenic agent, and combinations thereof. In some embodiments, the additional therapeutic agent is an ABP. 
     Diagnostic Methods 
     Also provided are methods for predicting and/or detecting the presence of a given HLA-PEPTIDE on a cell from a subject. Such methods may be used, for example, to predict and evaluate responsiveness to treatment with an ABP and/or cell provided herein. 
     In some embodiments, a blood or tumor sample is obtained from a subject and the fraction of cells expressing HLA-PEPTIDE is determined. In some aspects, the relative amount of HLA-PEPTIDE expressed by such cells is determined. The fraction of cells expressing HLA-PEPTIDE and the relative amount of HLA-PEPTIDE expressed by such cells can be determined by any suitable method. In some embodiments, flow cytometry is used to make such measurements. In some embodiments, fluorescence assisted cell sorting (FACS) is used to make such measurement. See Li et al.,  J. Autoimmunity,  2003, 21:83-92 for methods of evaluating expression of HLA-PEPTIDE in peripheral blood. 
     In some embodiments, detecting the presence of a given HLA-PEPTIDE on a cell from a subject is performed using immunoprecipitation and mass spectrometry. This can be performed by obtaining a tumor sample (e.g., a frozen tumor sample) such as a primary tumor specimen and applying immunoprecipitation to isolate one or more peptides. The HLA alleles of the tumor sample can be determined experimentally or obtained from a third party source. The one or more peptides can be subjected to mass spectrometry (MS) to determine their sequence(s). The spectra from the MS can then be searched against a database. An example is provided in the Examples section below. 
     In some embodiments, predicting the presence of a given HLA-PEPTIDE on a cell from a subject is performed using a computer-based model applied to the peptide sequence and/or RNA measurements of one or more genes comprising that peptide sequence (e.g., RNA seq or RT-PCR, or nanostring) from a tumor sample. The model used can be as described in international patent application no. PCT/US2016/067159, herein incorporated by reference, in its entirety, for all purposes. 
     Kits 
     Also provided are kits comprising an ABP and/or cell provided herein. The kits may be used for the treatment, prevention, and/or diagnosis of a disease or disorder, as described herein. 
     In some embodiments, the kit comprises a container and a label or package insert on or associated with the container. Suitable containers include, for example, bottles, vials, syringes, and IV solution bags. The containers may be formed from a variety of materials, such as glass or plastic. The container holds a composition that is by itself, or when combined with another composition, effective for treating, preventing and/or diagnosing a disease or disorder. The container may have a sterile access port. For example, if the container is an intravenous solution bag or a vial, it may have a port that can be pierced by a needle. At least one active agent in the composition is an ABP provided herein. The label or package insert indicates that the composition is used for treating the selected condition. 
     In some embodiments, the kit comprises (a) a first container with a first composition contained therein, wherein the first composition comprises an ABP and/or cell provided herein; and (b) a second container with a second composition contained therein, wherein the second composition comprises a further therapeutic agent. The kit in this embodiment can further comprise a package insert indicating that the compositions can be used to treat a particular condition, e.g., cancer. 
     Alternatively, or additionally, the kit may further comprise a second (or third) container comprising a pharmaceutically-acceptable excipient. In some aspects, the excipient is a buffer. The kit may further include other materials desirable from a commercial and user standpoint, including filters, needles, and syringes. 
     EXAMPLES 
     Below are examples of specific embodiments for carrying out the present invention. The examples are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way. Efforts have been made to ensure accuracy with respect to numbers used (e.g., amounts, temperatures, etc.), but some experimental error and deviation should, of course, be allowed for. 
     The practice of the present invention will employ, unless otherwise indicated, conventional methods of protein chemistry, biochemistry, recombinant DNA techniques and pharmacology, within the skill of the art. Such techniques are explained fully in the literature. See, e.g., T. E. Creighton,  Proteins: Structures and Molecular Properties  (W.H. Freeman and Company, 1993); A. L. Lehninger,  Biochemistry  (Worth Publishers, Inc., current addition); Sambrook, et al.,  Molecular Cloning: A Laboratory Manual  (2nd Edition, 1989);  Methods In Enzymology  (S. Colowick and N. Kaplan eds., Academic Press, Inc.);  Remington&#39;s Pharmaceutical Sciences,  18th Edition (Easton, Pa.: Mack Publishing Company, 1990); Carey and Sundberg  Advanced Organic Chemistry  3 rd  Ed. (Plenum Press) Vols A and B (1992). 
     Example 1: Identification of Predicted HLA-PEPTIDE Complexes (Table A) 
     We identified two classes of cancer specific HLA-peptide targets: The first class (cancer testis antigens, CTAs) are not expressed or are expressed at minimal levels in most normal tissues and expressed in tumor samples. The second class (tumor associated antigens, TAAs) are expressed highly in tumor samples and may have low expression in normal tissues. 
     We identified gene targets using three computational steps: First, we identified genes with low or no expression in most normal tissues using data available through the Genotype-Tissue Expression (GTEx) Project [1]. We obtained aggregated gene expression data from the Genotype-Tissue Expression (GTEx) Project (version V7p2). This dataset comprised 11,688 post-mortem samples from 714 individuals and fifty-three different tissue types. Expression was measured using RNA-Seq and computationally processed according to the GTEx standard pipeline (https://www.gtexportal.org/home/documentationPage). Gene expression was calculated using the sum of isoform expression that were calculated using RSEM v1.2.22 [2]. 
     Next, we identified which of those genes are aberrantly expressed in cancer samples using data from The Cancer Genome Atlas (TCGA) Research Network: http://cancergenome.nih.gov/. We examined 11,093 samples available from TCGA (Data Release 6.0). Because GTEx and TCGA use different annotations of the human genome in their computational analyses, we only included genes for which there were available ENCODE mappings between the two datasets. 
     Finally, in these genes, we identified which peptides are likely to be presented as cell surface antigens by MHC Class I proteins using a deep learning model trained on HLA presented peptides sequenced by tandem mass spectrometry (MS/MS), as described in international patent application no. PCT/US2016/067159, herein incorporated by reference, in its entirety, for all purposes. 
     Specific criteria for the two classes of genes is given below. 
     CTA Inclusion Criteria 
     To identify the CTAs, we sought to define a criteria to exclude genes that were expressed in normal tissue that was strict enough to ensure tumor specificity, but would not exclude non-zero measurements arising from potential artifacts such as read misalignment. Genes were eligible for inclusion as CTAs if they met the following criteria: The median GTEx expression in each organ that was a part of the brain, heart, or lung was less than 0.1 transcripts per million (TPM) with no one sample exceeding 5 TPM. The median GTEx expression in other essential organs was less than 2 TPM with no one sample exceeding 10 TPM. Expression was ignored for organs classified as non-essential (testis, thyroid, and minor salivary gland). Genes were considered expressed in tumor samples if they had expression in TCGA of greater than 20 TPM in at least 30 samples. 
     We then examined the distribution of the expression of the remaining genes across the TCGA samples. When we examined the known CTAs, e.g. the MAGE family of genes, we observed that the expression these genes in log space was generally characterized by a bimodal distribution. This distribution consisted of a left mode around a lower expression value and a right mode (or thick tail) at a higher expression level. This expression pattern is consistent with a biological model in which some minimal expression is detected at baseline in all samples and higher expression of the gene is observed in a subset of tumors experiencing epigenetic dysregulation. We reviewed the distribution of expression of each gene across TCGA samples and discarded those where we observed only a unimodal distribution with no significant right-hand tail. 
     TAA Inclusion Criteria 
     The TAAs were identified by focusing on genes with much higher expression in tumor tissues than in normal tissue: We first identified genes with a median TPM of less than 10 in all GTEx essential, normal tissues and then selected the subset which had expression of greater than 100 TPM in at least one TCGA tumor tissues. Then, we examined the distribution of each of these genes and selected those with a bimodal distribution of expression, as well as additional evidence of significantly elevated expression in one or more tumor types. 
     Lists were further reviewed to eliminate genes which are known to have expression in tissues not adequately represented in GTEx or which could have originated from immune cell infiltrates within the tumor. These steps left of us with a final list of 56 CTA and 58 TAA genes. 
     We also added peptides from two additional proteins known to be present in cancer. We added the junction peptides from the EGFR-SEPT14 fusion protein [3] and we added peptides from KLK3 (PSA). We also added peptides from two genes from the same gene family as PSA: KLK2 and KLK4. 
     To identify the peptides that are likely to be presented as cell surface antigens by MHC Class I proteins, we used a sliding window to parse each of these proteins into its constituent 8-11 amino acid sequences. We processed these peptides and their flanking sequences with the HLA peptide presentation deep learning model to calculate the likelihood of presentation of each peptide at the max expression level observed for this gene in TCGA. We considered a peptide likely to be presented (i.e., a candidate target) if its quantile normalized probability of presentation calculated by our model was greater than 0.001. 
     The results are shown in Table A. This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     In summary, the example provides a large set of tumor-specific HLA-PEPTIDEs that can be pursued as candidate targets for ABP development. 
     REFERENCES 
     
         
         1. Consortium, G. T.,  The Genotype - Tissue Expression  ( GTEx )  project . Nat Genet, 2013. 45(6): p. 580-5. 
         2. Li B, Dewey C N.,  RSEM: accurate transcript quantificationfrom RNA - Seq data with or without a reference genome . BMC Bioinformatics. 2011 Aug. 4; 12:323. 
         3. Frattini V, Trifonov V, Chan J M, Castano A, Lia M, Abate F, Keir S T, Ji A X, Zoppoli P, Niola F, Danussi C, Dolgalev I, Porrati P, Pellegatta S, Heguy A, Gupta G, Pisapia D J, Canoll P, Bruce I N, McLendon R E, Yan H, Aldape K, Finocchiaro G, Mikkelsen T, Prive G G, Bigner D D, Lasorella A, Rabadan R, Iavarone A.  The integrated landscape of driver genomic alterations in glioblastoma . Nat Genet. 2013 October; 45(10):1141-9. 
       
    
     Example 2: Validation of Predicted HLA-PEPTIDE Complexes 
     The presence of peptides from the HLA-PEPTIDE complexes of Tables A, A1, and A2 was determined using mass spectrometry (MS) on tumor samples known to be positive for each given HLA allele from the respective HLA-PEPTIDE complex. 
     Isolation of HLA-peptide molecules was performed using classic immunoprecipitation (IP) methods after lysis and solubilization of the tissue sample (1-4). Fresh frozen tissue was first frozen in liquid nitrogen and pulverized (CryoPrep; Covaris, Woburn, Mass.). One tenth of the sample was aliquoted for genomic sequencing efforts and lysis buffer (1% CHAPS, 20 mM Tris-HCl, 150 mM NaCl, protease and phosphatase inhibitors, pH=8) was added to solubilize the remaining pulverized tissue. The sample lysate was spun at 4° C. for 2 hrs to pellet debris. The clarified lysate was used for the HLA specific IP. 
     Immunoprecipitation was performed using antibodies coupled to beads where the antibody was specific for HLA molecules. For a pan-Class I HLA immunoprecipitation, the antibody W6/32 (5) was used, for Class II HLA-DR, antibody L243 (6) was used. Antibody was covalently attached to NHS-sepharose beads during overnight incubation. After covalent attachment, the beads were washed and aliquoted for IP. Additional methods for IP can be used including but not limited to Protein A/G capture of antibody, magnetic bead isolation, or other methods commonly used for immunoprecipitation. 
     The lysate was added to the antibody beads and rotated at 4° C. overnight for the immunoprecipitation. After immunoprecipitation, the beads were removed from the lysate and the lysate was stored for additional experiments, including additional IPs. The TP beads were washed to remove non-specific binding and the HLA/peptide complex was eluted from the beads with 2N acetic acid. The protein components were removed from the peptides using a molecular weight spin column or C18 cleanup step. The resultant peptides were taken to dryness by SpeedVac evaporation and can be stored at −20° C. prior to MS analysis. 
     Dried peptides were reconstituted in HPLC buffer A and loaded onto a C-18 microcapillary HPLC column for gradient elution in to the mass spectrometer. A gradient of 0-40% B (solvent A—0.1% formic acid, solvent B—0.1% formic acid in 80% acetonitrile) in 180 minutes was used to elute the peptides into the Fusion Lumos mass spectrometer (Thermo). MS1 spectra of peptide mass/charge (m/z) were collected in the Orbitrap detector with 120,000 resolution followed by 20 MS2 scans. Selection of MS2 ions was performed using data dependent acquisition mode and dynamic exclusion of 30 sec after MS2 selection of an ion. Automatic gain control (AGC) for MS1 scans was set to 4×105 and for MS2 scans was set to 1×104. For sequencing HLA peptides, +1, +2 and +3 charge states can be selected for MS2 fragmentation. Alternatively, MS2 spectra can be acquired using mass targeting methods where only masses listed in the inclusion list were selected for isolation and fragmentation. This was commonly referred to as Targeted Mass Spectrometry and was performed in either a qualitative manner or can be quantitative. Quantitation methods require each peptide to be quantitated to be synthesized using heavy labeled amino acids. (Doerr 2013) 
     MS2 spectra from each analysis were searched against a protein database using Comet (7-8) and the peptide identification was scored using Percolator (9-11) or using the integrated de novo sequencing and database search algorithm of PEAKS. Peptides from targeted MS2 experiments were analyzed using Skyline (Lindsay K. Pino et al. 2017) or other method to analyze predicted fragment ions. 
     The presence of multiple peptides from the predicted HLA-PEPTIDE complexes was determined using mass spectrometry (MS) on various tumor samples known to be positive for each given HLA allele from the respective HLA-PEPTIDE complex. 
     Representative spectra data for selected HLA-restricted peptides is shown in  FIGS. 51-63 . Each spectra contains the peptide fragmentation information as well as information related to the patient sample, including HLA types. 
     The spontaneous modification of amino acids can occur to many amino acids. Cysteine was especially susceptible to this modification and can be oxidized or modified with a free cysteine. Additionally N-terminal glutamine amino acids can be converted to pyro-glutamic acid. Since each of these modifications results in a change in mass, they can be definitively assigned in the MS2 spectra. To use these peptides in preparation of ABPs the peptide may need to contain the same modification as seen in the mass spectrometer. These modifications can be created using simple laboratory and peptide synthesis methods (Lee et al.; Ref 14). 
     REFERENCES 
     
         
         (1) Hunt D F, Henderson R A, Shabanowitz J, Sakaguchi K, Michel H, Sevilir N, Cox A L, Appella E, Engelhard V H. Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. Science 1992. 255: 1261-1263. 
         (2) Zarling A L, Polefrone J M, Evans A M, Mikesh L M, Shabanowitz J, Lewis S T, Engelhard V H, Hunt D F. Identification of class I MHC-associated phosphopeptides as targets for cancer immunotherapy._Proc Natl Acad Sci USA. 2006 Oct. 3; 103(40):14889-94. 
         (3) Bassani-Sternberg M, Pletscher-Frankild S, Jensen L J, Mann M. Mass spectrometry of human leukocyte antigen class I peptidomes reveals strong effects of protein abundance and turnover on antigen presentation. Mol Cell Proteomics. 2015 March; 14(3):658-73. doi: 10.1074/mcp.M114.042812. 
         (4) Abelin J G, Trantham P D, Penny S A, Patterson A M, Ward S T, Hildebrand W H, Cobbold M, Bai D L, Shabanowitz J, Hunt D F. Complementary IMAC enrichment methods for HLA-associated phosphopeptide identification by mass spectrometry. Nat Protoc. 2015 September; 10(9):1308-18. doi: 10.1038/nprot.2015.086. Epub 2015 Aug. 6 
         (5) Barnstable C J, Bodmer W F, Brown G, Galfre G, Milstein C, Williams A F, Ziegler A. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978 May; 14(1):9-20. 
         (6) Goldman J M, Hibbin J, Kearney L, Orchard K, Th&#39;ng K H. HLA-D R monoclonal antibodies inhibit the proliferation of normal and chronic granulocytic leukaemia myeloid progenitor cells. Br J Haematol. 1982 November; 52(3):411-20. 
         (7) Eng J K, Jahan T A, Hoopmann M R. Comet: an open-source M S/M S sequence database search tool. Proteomics. 2013 January; 13(1):22-4. doi: 10.1002/pmic.201200439. Epub 2012 Dec. 4. 
         (8) Eng J K, Hoopmann M R, Jahan T A, Egertson J D, Noble W S, MacCoss M J. A deeper look into Comet—implementation and features. J Am Soc Mass Spectrom. 2015 November; 26(11):1865-74. doi: 10.1007/s13361-015-1179-x. Epub 2015 Jun. 27. 
         (9) Lukas Kall, Jesse Canterbury, Jason Weston, William Stafford Noble and Michael J. MacCoss. Semi-supervised learning for peptide identification from shotgun proteomics datasets. Nature Methods 4:923-925, November 2007 
         (10) Lukas Kall, John D. Storey, Michael J. MacCoss and William Stafford Noble. Assigning confidence measures to peptides identified by tandem mass spectrometry. Journal of Proteome Research, 7(1):29-34, January 2008 
         (11) Lukas Kall, John D. Storey and William Stafford Noble. Nonparametric estimation of posterior error probabilities associated with peptides identified by tandem mass spectrometry. Bioinformatics, 24(16):i42-i48, August 2008 
         (12) Doerr, A. (2013) Mass Spectrometry-based targeted proteomics. Nature Methods, 10, 23. 
         (13) Lindsay K. Pino, Brian C. Searle, James G. Bollinger, Brook Nunn, Brendan MacLean &amp; M. J. MacCoss (2017) The Skyline ecosystem: Informatics for quantitative mass spectrometry proteomics. Mass Spectrometry Reviews. 
         (14) Lee W Thompson; Kevin T Hogan; Jennifer A Caldwell; Richard A Pierce; Ronald C Hendrickson; Donna H Deacon; Robert E Settlage; Laurence H Brinckerhoff, Victor H Engelhard; Jeffrey Shabanowitz; Donald F Hunt; Craig L Slingluff. Preventing the spontaneous modification of an HLA-A2-restricted peptide at an N-terminal glutamine or an internal cysteine residue enhances peptide antigenicity. Journal of Immunotherapy (Hagerstown, Md.: 1997). 27(3):177-83, MAY 2004. 
       
    
     Example 3: Identification of Antibodies and Antigen Binding Fragments Thereof that Bind HLA-PEPTIDE Targets HLA-B*35:01 EVDPIGHVY, HLA-A*02:01 AIFPGAVPAA, and HLA-A*01:01 ASSLPTTMNY 
     Overview 
     The following exemplification demonstrates that antibodies (Abs) can be identified that recognize tumor-specific HLA-restricted peptides. The overall epitope that is recognized by such Abs generally comprises a composite surface of both the peptide as well as the HLA protein presenting that particular peptide. Abs that recognize HLA complexes in a peptide-specific manner are often referred to as T cell receptor (TCR)-like Abs or TCR-mimetic Abs. The HLA-PEPTIDE target antigens that were selected for antibody discovery, derived from the tumor-specific gene product MAGEA6, FOXE1, MAGE3/6, were HLA-B*35:01_EVDPIGHVY (HLA-PEPTIDE target “G5”), HLA-A*02:01_AIFPGAVPAA (HLA-PEPTIDE target “G8”), and HLA-A*01:01_ASSLPTTMNY (HLA-PEPTIDE target “G10”), respectively. Cell surface presentation of these HLA-PEPTIDE targets was confirmed by mass spectrometry analysis of HLA complexes obtained from tumor samples as described in Example 2. Representative plots are depicted in  FIGS. 25-27 . 
     HLA-PEPTIDE Target Complexes and Counterscreen Peptide-HLA Complexes 
     The HLA-PEPTIDE targets G5, G8, G10, as well as counterscreen negative control peptide-HLAs, were produced recombinantly using conditional ligands for HLA molecules using established methods. In all, 18 counterscreen HLA-peptides were generated for each of the HLA-PEPTIDE targets. The 18 counterscreen HLA-peptides were designed such that (A) the negative control peptide was known to be presented by the same HLA subtype (i.e. the HLA-related controls) or (B) the negative control peptides were known to be presented by a different HLA subtype. The grouping of the target and the negative control peptide-HLA complexes for screen 1 is shown in  FIG. 3  (with detailed sequence information provided in Table 1), and for screen 2 shown in  FIG. 4  (with detailed sequence information provided in Table 2. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 HLA-PEPTIDE sequence design for Screen 1 negative control peptides and 
               
               
                 ″G5″ target 
               
            
           
           
               
               
               
               
               
            
               
                 Group 
                 HLA 
                 Peptide 
                 Gene 
                 Target 
               
               
                   
               
               
                 G1 
                 HLA-A*02:01 
                 LLFGYPVYV 
                   
                 Neg Ctrl 1 
               
               
                   
                 HLA-A*02:01 
                 GILGFVFTL 
                   
                 Neg Ctrl 2 
               
               
                   
                 HLA-A*02:01 
                 FLLTRILTI 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G2 
                 HLA-A*01:01 
                 YSEHPTFTSQY 
                   
                 Neg Ctrl 1 
               
               
                   
                 HLA-A*01:01 
                 VSDGGPNLY 
                   
                 Neg Ctrl 2 
               
               
                   
                 HLA-A*01:01 
                 ATDALMTGY 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G3 
                 HLA-A*11:01 
                 IVTDFSVIK 
                   
                 Neg Ctrl 1 
               
               
                   
                 HLA-A*11:01 
                 KSMREEYRK 
                   
                 Neg Ctrl 2 
               
               
                   
                 HLA-A*11:01 
                 SSCSSCPLSK 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G4 
                 HLA-A*11:01 
                 ATIGTAMYK 
                   
                 Neg Ctrl 1 
               
               
                   
                 HLA-A*11:01 
                 AVFDRKSDAK 
                   
                 Neg Ctrl 2 
               
               
                   
                 HLA-A*11:01 
                 SIIPSGPLK 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G5 
                 HLA-B*35:01 
                 EVDPIGHVY 
                 MAGEA6 
                 Target 
               
               
                   
                 HLA-B*35:01 
                 IPSINVHHY 
                   
                 Neg Ctrl 1 
               
               
                   
                 HLA-B*35:01 
                 EPLPQGQLTAY 
                   
                 Neg Ctrl 2 
               
               
                   
                 HLA-B*35:01 
                 VPLDEDFRKY 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G6 
                 HLA-A*03:01 
                 RLRAEAQVK 
                   
                 Neg Ctrl 1 
               
               
                   
                 HLA-A*03:01 
                 RLRPGGKKK 
                   
                 Neg Ctrl 2 
               
               
                   
                 HLA-A*03:01 
                 QVPLRPMTYK 
                   
                 Neg Ctrl 3 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 HLA-PEPTIDE sequence design for Screen 2 negative control peptides, G8, 
               
               
                 and G10 targets* 
               
            
           
           
               
               
               
               
               
            
               
                 Group 
                 HLA 
                 Peptide 
                 Gene 
                 Target 
               
               
                   
               
               
                 G7/G8‡ 
                 A*02:01 
                 LLFGYPVYV 
                   
                 Neg Ctrl 1 
               
               
                   
                 A*02:01 
                 GILGFVFTL 
                   
                 Neg Ctrl 2 
               
               
                   
                 A*02:01 
                 FLLTRILTI 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G9 
                 A*24:02 
                 TYGPVFMCL 
                   
                 Neg Ctrl 1 
               
               
                   
                 A*24:02 
                 RYLKDQQLL 
                   
                 Neg Ctrl 2 
               
               
                   
                 A*24:02 
                 PYLFWLAAI 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G10 
                 A*01:01 
                 ASSLPTTMNY 
                 MAGE3/6 
                 Target 
               
               
                   
                 A*01:01 
                 YSEHPTFTSQY 
                   
                 Neg Ctrl 1 
               
               
                   
                 A*01:01 
                 VSDGGPNLY 
                   
                 Neg Ctrl 2 
               
               
                   
                 A*01:01 
                 ATDALMTGY 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G11 (=G3) 
                 A*11:01 
                 IVTDFSVIK 
                   
                 Neg Ctrl 1 
               
               
                   
                 A*11:01 
                 KSMREEYRK 
                   
                 Neg Ctrl 2 
               
               
                   
                 A*11:01 
                 SSCSSCPLSK 
                   
                 Neg Ctrl 3 
               
               
                   
               
               
                 G12 (=G6) 
                 A*03:01 
                 RLRAEAQVK 
                   
                 Neg Ctrl 1 
               
               
                   
                 A*03:01 
                 RLRPGGKKK 
                   
                 Neg Ctrl 2 
               
               
                   
                 A*03:01 
                 QVPLRPMTYK 
                   
                 Neg Ctrl 3 
               
               
                   
               
            
           
         
       
     
     Generation and Stability Analysis of HLA-PEPTIDE Target Complexes and Counterscreen Peptide-HLA Complexes 
     Results for the G5 counterscreen “minipool” and G2 target are shown in  FIG. 5 . All three counterscreen peptides and the G5 peptide rescued the HLA complex from dissociation. 
     Results for the additional G5 “complete” pool counterscreen peptides are shown in  FIG. 6 , demonstrating that they also form stable HLA-peptide complexes. 
     Results for counterscreen peptides and G8 target are shown in  FIG. 7 . All three counterscreen peptides and the G8 peptide rescued the HLA complex from dissociation. 
     Results for the G10 counterscreen “minipool” and G10 target are shown in  FIG. 8 . All three counterscreen peptides and the G10 peptide rescued the HLA complex from dissociation. 
     Results for the additional G8 and G10 “complete” pool counterscreen peptides are shown in  FIG. 9 , demonstrating that they also form stable HLA-peptide complexes. 
     Phage Library Screening 
     The highly diverse SuperHuman 2.0 synthetic naïve scFv library from Distributed Bio Inc was used as input material for phage display, which has a 7.6×10 10  total diversity on ultra-stable and diverse VH/VL scaffolds. For both screen 1 (see  FIG. 3 ) and screen 2 (see  FIG. 4 ) three to four rounds of bead-based phage panning with the target pHLA complex (as shown in Table 3) were conducted using established protocols to identify scFv binders to pHLAs G5, G8 and G10, respectively. For each round of panning, the phage library was initially depleted with 18 pooled negative pHLA complexes prior to the binding step with the target pHLAs. The phage titer was determined at every round of panning to establish removal of non-binding phage. The output phage supernatant was also tested for target binding by ELISA and suggested progressive enrichment of G5-, G8 and G10 binding phage (see  FIG. 10 ). 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Phage library screening strategy 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Antigen  
                   
               
               
                   
                 Round 
                 concentration 
                 Washes 
               
               
                   
                   
               
               
                   
                 R1 
                 100 pmol 
                 3X PBST + 3X PBS (5 min washes) 
               
               
                   
                 R2 
                  25 pmol 
                 5 PBST (2x 30 sec, 3x 5 min) +  
               
               
                   
                   
                   
                 5 PBS (2x 30 sec, 3x 5 min) 
               
               
                   
                 R3 
                  10 pmol 
                 8 PBST (4x 30 sec, 4x 5 min) +  
               
               
                   
                   
                   
                 8 PBS (4x 30 sec, 4x 5 min) 
               
               
                   
                 R4 
                 5 pmol, 10 pmol 
                 30 min PBST + 30 min PBS 
               
               
                   
                   
               
            
           
         
       
     
     Bacterial periplasmic extracts (PPEs) of individual output clones were subsequently generated in 96-well plates using well-established protocols. The PPEs were used to test for binding to the target pHLA antigen by high throughput PPE ELISA. Positive clones were sequenced and re-arrayed to select sequence-unique clones. Sequence unique clones were then tested in a secondary ELISA for binding to target pHLA versus the panel of HLA-matched negative control pHLA complexes, thus establishing target specificity. The G8 negative control HLA complexes (i.e. A*24:02) did not HLA-match with the G8 target HLA complex (i.e. A*02:01). Therefore, HLA-A*02:01 complexes presenting the peptides LLFGYPVYV, GILGFVFTL or FLLTRILTI from G7 were used as HLA-matched minipool of negative controls for G8 in further biochemical and functional characterization assays for the TCR-mimetic Abs retrieved from the scFv library. 
     Isolation of scFv Hits 
     Individual, soluble scFv protein fragments were produced and purified for the scFv clones that were found to be selective when expressed in PPEs. As shown by scFv PPE ELISA, these clones exhibited at least three-fold selective binding to the target pHLA as compared to binding to the minipool of negative control pHLAs. Soluble scFv production allowed for further biochemical and functional characterization. 
     The resulting VH and VL sequences for the scFvs that bind target G5 are shown in Table 4. To clarify the organization of Table 4, and other Tables of scFv sequences, each scFv was assigned a clone name. For all clone names, clone names recite the target (e.g., G5), the plate number (e.g., plate 7), and well number (e.g., well E7) of the 96-well plate from which the clone was originally picked. For example, clone names G5-P7E07, G5-7E7, G5(7E7), G5(7E07) G5_P7_E7, all refer to the same scFv clone. For example, Table 4 indicates that the scFv from clone G5_P7_E7 has the VH sequence QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYDINWVRQAPGQGLEWMGIINPRSG STKYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARDGVRYYGMDVWG QGTTVTVSSAS and the VL sequence 
     
       
         
           
               
            
               
                 DIVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGYNYLDWYLQKPGQSPQ 
               
               
                 LLIYLGSYRASGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCMQGLQTP 
               
               
                 ITFGQGTRLEIK. 
               
            
           
         
       
     
     The resulting CDR sequences for the scFvs that bind target G5 are shown in Table 5. To clarify the organization of Table 5, each scFv was assigned a clone name in Table 5. For example, the scFv from clone G5_P7_E7 has an HCDR1 sequence that is YTFTSYDIN, an HCDR2 sequence that is GIINPRSGSTKYA, an HCDR3 sequence that is CARDGVRYYGMDVW, an LCDR1 sequence that is RSSQSLLHSNGYNYLD, an LCDR2 sequence that is LGSYRAS, and an LCDR3 sequence that is CMQGLQTPITF, according to the Kabat numbering system. 
     The resulting VH and VL sequences for the scFvs that bind target G8 are shown in Table 6. Table 6 is organized similarly to Table 4. 
     The resulting CDR sequences for the scFvs that bind target G8 are shown in Table 7. Table 7 is organized similarly to Table 5. 
     The resulting VH and VL sequences for the scFvs that bind target G10 are shown in Table 8. Table 8 is organized similarly to Table 4. 
     The resulting CDR sequences for the scFvs that bind target G8 are shown in Table 9. Table 9 is organized similarly to Table 5. 
     A number of clones were formatted into scFv, Fab, and IgG to facilitate biochemical, structural, and functional characterization (see Table 10). 
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Hit rate of the screening campaigns. Clones were reformatted  
               
               
                 into (a) IgG for biochemical and functional  
               
               
                 characterization, (b) Fab constructs for protein 
               
               
                 crystallography and HDX mass spectrometry,  
               
               
                 and (c) scFv constructs for HDX mass 
               
               
                 spectrometry. 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Group 
                 G5 
                 G8 
                 G10 
               
               
                   
                   
               
               
                   
                 HLA 
                 B*35:01 
                 A*02:01 
                 A*01:01 
               
               
                   
                 Peptide 
                 MAGEA6 
                 FOXE1 
                 MAGE3/6 
               
               
                   
                 Sequence Unique 
                 81 
                 17 
                 23 
               
               
                   
                 Binders 
                   
                   
                   
               
               
                   
                 Selective Binders 
                 18 
                 17 
                 18 
               
               
                   
                 IgG 
                 18 
                 17 
                 18 
               
               
                   
                 Fab 
                 4 
                 3 
                 2 
               
               
                   
                 scFv 
                 8 
                 7 
                 6 
               
               
                   
                   
               
            
           
         
       
     
       FIG. 11  depicts a flow chart describing the antibody selection process, including criteria and intended application for the scFv, Fab, and IgG formats. Briefly, clones were selected for further characterization based on sequence diversity, binding affinity, selectivity, and CDR3 diversity. 
     To assess sequence diversity, dendrograms were produced using clustal software. The predicted 3D structures of the scFv sequences, based on the VH type, were also taken into consideration. Binding affinity as determined by the equilibrium dissociation constant (K D ) was measured using an Octet HTX (ForteBio). Selectivity for the specific peptide-HLA complexes was determined with an ELISA titration of the purified scFvs as compared to the minipool of negative control pHLA complexes or streptavidin alone. Cutoff values for the K D  and selectivity were determined for each target set based on the range of values obtained for the Fabs within each set. Final clones were selected based on diversity in sequence families and CDR3 sequences. 
     The overall number of hits following phage library screening and scFv isolation are listed in Table 10, above. 
     Materials and Methods 
     HLA Expression and Purification: 
     Recombinant proteins were obtained through bacterial expression using established procedures (Garboczi, Hung, &amp; Wiley, 1992). Briefly, the α chain and β2 microglobulin chain of various human leukocyte antigens (HLA) were expressed separately in BL21 competent  E. Coli  cells (New England Biolabs). Following auto-induction, cells were lysed via sonication in Bugbuster® plus benzonase protein extraction reagent (Novagen). The resulting inclusion bodies were washed and sonicated in wash buffer with and without 0.5% Triton X-100 (50 mM Tris, 100 mM NaCl, 1 mM EDTA). After the final centrifugation, inclusion pellets were dissolved in urea solution (8 M urea, 25 mM MES, 10 mM EDTA, 0.1 mM DTT, pH 6.0). Bradford assay (Biorad) was used to quantify the concentration and the inclusion bodies were stored at −80° C. 
     Refold of pHLA and Purification: 
     HLA complexes were obtained by refolding of recombinantly produced subunits and a synthetically obtained peptide using established procedures. (Garboczi et al., 1992) Briefly, the purified α and β2 microglobulin chains were refolded in refold buffer (100 mM Tris pH 8.0, 400 mM L-Arginine HCl, 2 mM EDTA, 50 mM oxidized glutathione, 5 mM reduced glutathione, protease inhibitor tablet) with either the target peptide or a cleavable ligand. The refold solution was concentrated with a Vivaflow 50 or 50R crossflow cassette (Sartorius Stedim). Three rounds of dialyses in 20 mM Tris pH 8.0 were performed for at least 8 hours each. For the antibody screening and functional assays, the refolded HLA was enzymatically biotinylated using BirA biotin ligase (Avidity). Refolded protein complexes were purified using a HiPrep (16/60 Sephacryl 5200) size exclusion column attached to an AKTA FPLC system. Biotinylation was confirmed in a streptavidin gel-shift assay under non-reducing conditions by incubating the refolded protein with an excess of streptavidin at room temperature for 15 minutes prior to SDS-PAGE. The peptide-HLA complexes were aliquoted and stored at −80° C. 
     Peptide Exchange: 
     HLA-peptide stability was assessed by conditional ligand peptide exchange and stability ELISA assay. Briefly, conditional ligand-HLA complexes were subjected to conditional stimulus in the presence or absence of the counterscreen or test peptides. Exposure to the conditional stimulus cleaves the conditional ligand from the HLA complex, resulting in dissociation of the HLA complex. If the counterscreen or test peptide stably binds the α1/α2 groove of the HLA complex, it “rescues” the HLA complex from disassociation. In short, a mixture of 100 μL of 50 μM of the novel peptide (Genscript) and 0.5 μM recombinantly produced cleavable ligand-loaded HLA in 20 mM Tris HCl and 50 mM NaCl at pH 8 was placed on ice. The mixture was irradiated for 15 min in a UV cross-linker (CL-1000, UVP) equipped with 365-nm UV lamps at ˜10 cm distance. 
     MHC Stability Assay: 
     The MHC stability ELISA was performed using established procedures. (Chew et al., 2011; Rodenko et al., 2006) A 384-well clear flat bottom polystyrene microplate (Corning) was precoated with 50 μl of streptavidin (Invitrogen) at 2 μg/mL in PBS. Following 2 h of incubation at 37° C., the wells were washed with 0.05% Tween 20 in PBS (four times, 50 μL) wash buffer, treated with 50 μl of blocking buffer (2% BSA in PBS), and incubated for 30 min at room temperature. Subsequently, 25 μl of peptide-exchanged samples that were 300× diluted with 20 mM Tris HCl/50 mM NaCl were added in quadruplicate. The samples were incubated for 15 min at RT, washed with 0.05% Tween wash buffer (4×50 L), treated for 15 min with 25 μL of HRP-conjugated anti-02m (1 μg/mL in PBS) at RT, washed with 0.05% Tween wash buffer (4×50 μL), and developed for 10-15 min with 25 L of ABTS-solution (Invitrogen). The reactions were stopped by the addition of 12.5 μL of stop buffer (0.01% sodium azide in 0.1 M citric acid). Absorbance was subsequently measured at 415 nm using a spectrophotometer (SpectraMax i3x; Molecular Devices). 
     Phage Panning: 
     For each round of panning, an aliquot of starting phage was set aside for input titering and the remaining phage was depleted three times against Dynabead M-280 streptavidin beads (Life Technologies) followed by a depletion against Streptavidin beads pre-bound with 100 pmoles of pooled negative peptide-HLA complexes. For the first round of panning, 100 pmoles of peptide-HLA complex bound to streptavidin beads was incubated with depleted phage for 2 hours at room temperature with rotation. Three five-minute washes with 0.5% BSA in 1×PBST (PBS+0.05% Tween-20) followed by three five-minute washes with 0.5% BSA in 1×PBS were utilized to remove any unbound phage to the peptide-HLA complex bound beads. To elute the bound phage from the washed beads, 1 mL 0.1M TEA was added and incubated for 10 minutes at room temperature with rotation. The eluted phage was collected from the beads and neutralized with 0.5 mL 1M Tris-HCl pH 7.5. The neutralized phage was then used to infect log growth TG-1 cells (OD 600 =0.5) and after an hour of infection at 37° C., cells were plated onto 2YT media with 100 μg/mL carbenicillin and 2% glucose (2YTCG) agar plates for output titer and bacterial growth for subsequent panning rounds. For subsequent rounds of panning, selection antigen concentrations were lowered while washes increased by amount and length of wash times at show in Table 3. 
     Input Output Phage Titer: 
     Each round of input titer was serially diluted in 2YT media to 10 10 . Log phase TG-1 cells are infected with diluted phage titers (10 7 -10 10 ) and incubated at 37° C. for 30 minutes without shaking followed by another 30 minutes with gentle shaking. Infected cells are plated onto 2YTCG plates and incubated overnight at 30° C. Individual colonies were counted to determine input titer. Output titers were performed following 1 h infection of eluted phage into TG-1 cells. 1, 0.1, 0.01, and 0.001 μL of infected cells were plated onto 2YTCG platers and incubated overnight at 30° C. Individual colonies were counted to determine output titer. 
     Selective Target Binding of Bacterial Periplasmic Extracts: 
     For scFv PPE ELISAs, 96-well and/or 384-well streptavidin coated plates (Pierce) were coated with 2 μg/mL peptide-HLA complex in HLA buffer and incubated overnight at 4° C. Plates were washed three times between each step with PBST (PBS+0.05% Tween-20). The antigen coated plates were blocked with 3% BSA in PBS (blocking buffer) for 1 hour at room temperature. After washing, scFv PPEs were added to the plates and incubated at room temperature for 1 hour. Following washing, mouse anti-v5 antibody (Invitrogen) in blocking buffer was added to detect scFv and incubated at room temperature for 1 hour. After washing, HRP-goat anti-mouse antibody (Jackson ImmunoResearch) was added and incubated at room temperature for 1 hour. The plates were then washed three times with PBST and 3 times with PBS before HRP activity was detected with TMB 1-component Microwell Peroxidase Substrate (Seracare) and neutralized with 2N sulfuric acid. 
     For negative peptide-HLA complex counterscreening, the scFv PPE ELISAs were performed as described above, except for the coating antigen. Namely, the HLA mini-pools (see Tables 1 and 2) were used that consisted of 2 μg/mL of each of the three negative peptide-HLA complexes pooled and coated onto streptavidin plates for comparison binding to their particular pHLA complex. Alternatively, HLA complete pools consisted of 2 μg/mL of each of all 18 negative peptide-HLA complexes pooled together and coated onto streptavidin plates for comparison binding to their particular pHLA complex. 
     Construction and Production of scFv Protein Fragments: 
     The expression plasmid was transformed into BL21(DE3) strain and co-expressed with a periplasmic chaperone in a 400 mL  E. coli  culture. The cell pellet was reconstituted as follows: 10 mL/1 g biomass with (25 mM HEPES, pH7.4, 0.3M NaCl, 10 mM MgCl2, 10% glycerol, 0.75% CHAPS, 1 mM DTT) plus lysozyme, and benzonase and Lake Pharma protease inhibitor cocktail. The cell suspension was incubated on a shaking platform at RT for 30 minutes. Lysates were clarified by centrifugation at 4° C., 13,000×rpm for 15 min. The clarified lysate was loaded onto 5 mL of Ni NTA resin pre-equilibrated in IMAC Buffer A (20 mM Tris-HCl, Ph7.5; 300 mM NaCl/10% Glycerol/1 mM DTT). The resin was washed with 10 column volumes (CVs) of Buffer A (or until a stable baseline was reached), followed by 10 CVs of 8% IMAC Buffer B (20 mM Tris-HCl, Ph7.5; 300 mM NaCl/10% Glycerol/1 mM DTT/250 mM Imidazole). The target protein was eluted in a 20CV gradient to 100% IMAC Buffer B. The column was washed with 5CVs of 100% IMAC B to ensure complete protein removal. Elution fractions were analyzed by SDS-PAGE and Western blot (anti-His) and pooled accordingly. The pool was dialyzed with the final formulation buffer (20 mM Tris-HCl, Ph7.5; 300 mM NaCl/10% glycerol/1 mM DTT), concentrated to a final protein concentration &gt;0.3 mg/mL, aliquoted into 1 mL vials, and flash frozen in liquid nitrogen. Final QC steps included SDS-PAGE and A280 absorbance measurements. 
     Construction and Production of Fab Protein Fragments: 
     The constructs of selected G5, G8 and G10 Fabs were cloned into a vector optimized for mammalian expression. Each DNA construct was scaled up for transfection and sequences were confirmed. A 100 mL transient production was completed in HEK293 cells (Tuna293™ Process) for each. The proteins were purified by anti-CH1 purification subsequently purified by size exclusion chromatography (SEC) via HiLoad 16/600 Superdex 200. The mobile phase used for SEC-polishing was 20 mM Tris, 50 mM NaCl, pH 7. Final confirmatory CE-SDS analysis was performed. 
     Construction and Production of IgG Proteins: 
     The expression constructs of the G series antibodies were cloned into a vector optimized for mammalian expression. Each DNA construct was scaled up for transfection and sequences were confirmed. A 10 mL transient production was completed in HEK293 cells (Tuna293™ Process) for each. The proteins were purified by Protein A purification and final CE-SDS analysis was performed. 
     Example 4: Affinity of Fab Clones for their Respective HLA-PEPTIDE Targets 
     Fab-formatted antibodies allow for accurate assessment of monomeric binding to their respective HLA-PEPTIDE targets, while avoiding confounding effects of bivalent interactions with the IgG antibody format. Binding affinity was assessed by bio-layer interferometry (BLI) using an Octet Qke (ForteBio). Briefly, biotinylated pHLA complexes in kinetics buffer were loaded onto streptavidin sensors for 300 seconds, at concentrations which gave the optimal nm shift response (approximately 0.6 nm) for each Fab at the highest concentration used. The ligand-loaded tips were subsequently equilibrated in the kinetics buffer for 120 seconds. The ligand-loaded biosensors were then dipped for 200 seconds in the Fab solution titrated into 2-fold dilutions. Starting Fab concentrations ranged from 100 nM to 2 μM, iteratively optimized based on the K D  values of the Fab. The dissociation step in the kinetics buffer was measured for 200 seconds. Data were analyzed using the ForteBio data analysis software using a 1:1 binding model. 
     Results for HLA-PEPTIDE targets HLA-1B*35:01_EVDPIGHVY, HLA-A*02:01 AIFPGAVPAA, and HLA-A*01:01 ASSLPTTMNY are shown in Table 11, below. 
     
       
         
           
               
             
               
                 TABLE 11 
               
             
            
               
                   
               
               
                 Optimized Octet BLI affinity measurements of Fabs  
               
               
                 binding to their target peptide-HLA complex 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 Kon  
                 Kdis  
                 Full  
               
               
                 Target 
                 Fab clone 
                 KD (M) 
                 (1/Ms) 
                 (1/s) 
                 RA{circumflex over ( )}2 
               
               
                   
               
               
                 G5 
                 G5-P7A05 
                 1.19E−07 
                 4.10E+05 
                 4.87E−02 
                 0.997 
               
               
                 G5 
                 G5-P7B3 
                 2.54E−07 
                 4.42E+05 
                 9.09E−02 
                 0.993 
               
               
                 G5 
                 G5-P7E7 
                 2.82E−08 
                 9.02E+05 
                 2.48E−02 
                 0.991 
               
               
                 G5 
                 G5-P7F6 
                 3.37E−08 
                 9.15E+05 
                 3.06E−02 
                 0.995 
               
               
                 G5 
                 G5-P1C12 
                 8.59E−08 
                 4.93E+03 
                 1.58E−02 
                 0.983 
               
               
                 G8 
                 G8-P4F05 
                 9.84E−07 
                 6.40E+04 
                 6.30E−02 
                 0.976 
               
               
                 G8 
                 G8-P1B03 
                 3.07E−07 
                 1.67E+05 
                 5.11E−02 
                 0.996 
               
               
                 G8 
                 G8-P5G08 
                 5.30E−07 
                 9.97E+04 
                 5.28E−02 
                 0.927 
               
               
                 G8 
                 R3G8-P2C10 
                 1.77E−08 
                 7.50E+04 
                 1.30E−03 
                 0.997 
               
               
                 G8 
                 R3G8-P1C11 
                 1.78E−07 
                 1.90E+05 
                 3.38E−02 
                 0.997 
               
               
                 G8 
                 R3G8-P2E04 
                 2.86E−07 
                 5.45E+05 
                 7.89E−02 
                 0.842 
               
               
                 G10 
                 R3G10-P1B07 
                 3.75E−08 
                 1.65E+05 
                 6.15E−03 
                 0.997 
               
               
                 G10 
                 R3G10-P4E07 
                 4.28E−07 
                 4.77E+05 
                 1.11E−01 
                 0.990 
               
               
                   
               
            
           
         
       
     
       FIGS. 12A, 121B, and 12C  depicts BLI results for Fab clone G5-P7A05 to HLA-PEPTIDE target B*35:01-EVDPGHVY ( 12 A), Fab clones R3G8-P2C10 and G8-P1C11 to HLA-PEPTIDE target A*02:01-AFPGAVPAA ( 12 B, P2C10 on left and P3C11 on right), and Fab clone R3G10-P1B07 to HLA-PEPTIDE target A*01:01-ASSLPTTMNY ( 12 C), respectively. 
       FIGS. 71A and 71B  show LI results for G2 target Fab clone G2-P1H11 and for G7 target Fab clone G7R4-B5-P2E9, respectively.  FIG. 90  shows L results for G2 target Fab clone G2-P2C06. 
     Results are shown in the Table below. 
     
       
         
           
               
             
               
                 TABLE 43 
               
             
            
               
                   
               
               
                 Optimized Octet BLI affinity measurements of Fabs binding  
               
               
                 to their target peptide-HLA complex 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 Kon  
                   
                 Full 
               
               
                 Target 
                 Fab clone 
                 KD (M) 
                 (1/Ms) 
                 Kdis (1/s) 
                 R{circumflex over ( )}2 
               
               
                   
               
               
                 G2 
                 G2-P1B06 
                 4.44E−08 
                 1.06E+06 
                 3.23E−02 
                 0.991 
               
               
                 G2 
                 G2-P2A03 
                 1.09E−07 
                 3.32E+05 
                 3.60E−02 
                 0.998 
               
               
                 G2 
                 G2-P1B12 
                 2.28E−08 
                 3.66E+05 
                 7.28E−03 
                 0.980 
               
               
                 G2 
                 G2-P2A11 
                 2.81E−08 
                 6.33E+05 
                 1.72E−02 
                 0.992 
               
               
                 G2 
                 G2-P1H01 
                 1.55E−08 
                 9.52E+05 
                 1.48E−02 
                 0.984 
               
               
                 G2 
                 G2-P1H11 
                 4.99E−08 
                 5.81E+05 
                 2.80E−02 
                 0.994 
               
               
                 G2 
                 G2-P2C06 
                 3.06 E−08 
                 1.14 E+06 
                 3.48 E−02 
                 0.992 
               
               
                 G7 
                 2-G7R4-P2C2 
                 5.31E−07 
                 1.04E+05 
                 5.43E−02 
                 0.986 
               
               
                 G7 
                 3-G7R4-P1A3 
                 5.32E−07 
                 1.97E+05 
                 9.94E−02 
                 0.988 
               
               
                 G7 
                 4-G7R4-B5- 
                 1.18E−08 
                 1.85E+05 
                 2.12E−03 
                 0.992 
               
               
                   
                 P2E9 
               
               
                   
               
            
           
         
       
     
       FIG. 105  shows BLI results for G8 target Fab clones G8-P4F05, G8-P1B03, and G8-P5G08 to HLA-PEPTIDE target A*02:01-AIFPGAVPAA; as well as BLI results for G5 target Fab clone G5-P1C12 to HLA-PEPTIDE target B*35:01-EVDPIGHVY. 
     The Fab-formatted antibodies bind to their respective HLA-PEPTIDE targets with high affinity. 
     Example 5: Positional Scanning of G2, G5, G7, G8, and G10 Restricted Peptide Sequences 
     Positional scanning of the G2, G5, G7, G8, and G10 restricted peptides was carried out to determine the amino acid residues which act as contact points for selected Fab clones or residues that impact, directly or indirectly, the interaction of the HLA-PEPTIDE target with the Fab. 
       FIG. 13  depicts a first experimental design for the positional scanning experiments. Positional scanning libraries of variant G2, G5, G7, G8, and G10 restricted peptides were generated with amino acid substitutions at a single position in the restricted peptide sequence, scanning across all positions. The amino acid substitutions at a given position were either alanine (conservative substitution), arginine (positively charged), or aspartate (negatively charged). 
     Peptide-HLA complexes comprising the positional scanning library members and the HLA subtype allele were generated as described in Example 3. Stability of the resulting complexes was determined using conditional ligand peptide exchange and stability ELISA as described in Example 3. Such stability analysis may identify residues on the restricted peptide which are important for binding and stabilizing the HLA molecule. Binding affinity of the selected Fab clone to the variant peptide-HLA complexes was assessed by BLI as described in Example 4. Positional variants that result in stable HLA complex formation and weakened Fab binding may identify residues that are likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone. For instance, the identified residues may form part of the epitope that binds the ABP, or alternatively may influence the conformational shape or presentation of the epitope. 
       FIG. 14A  depicts stability results for the G5 positional variant-HLAs, indicating that the majority of peptide mutations does not impact binding of those peptides to the relevant pHLA. 
       FIG. 14B  depicts binding affinity of Fab clone G5-P7A05 to the G5 positional variant-HLAs, indicating positions P2-P8 of the restricted peptide as likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone. 
       FIG. 15A  depicts stability results for the G8 positional variant-HLAs, indicating that positions P2, P7 and P10 were not amenable to substitution with the Arg- or Asp-residue and therefore are likely to be important for the peptide to bind the HLA protein. 
       FIG. 15B  depicts binding affinity of Fab clone G8-P2C10 to the G8 positional variant-HLAs, indicating positions P1-P5 of the restricted peptide as likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone. 
       FIG. 46  depicts binding affinity of Fab clone G8-P1C11 to the G8 positional variant-HLAs, indicating positions P3-P6 of the restricted peptide as likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone. 
       FIG. 16A  depicts stability results for the G10 positional variant-HLAs, indicating that positions 2, 5, 8, and 10 were not amenable to amino acid substitution and therefore are likely to be important for the peptide to bind the HLA protein. 
       FIG. 16B  depicts binding affinity of Fab clone G10-P1B07 to the G10 positional variant-HLAs, indicating positions P4, P6, and P7 of the restricted peptide as likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone. 
     A map of the amino acid substitutions for the positional scanning experiments for G2 and G7 restricted peptides is shown in  FIG. 72 . Asterisks denote lack of amino acid substitution. 
       FIG. 73A  depicts stability results for the G2 positional variant-HLAs, indicating that positions 2, 3, and 9 were not amenable to amino acid substitutions and therefore are likely to be important for the peptide to bind the HLA protein. 
       FIG. 73B  depicts binding affinity of Fab clone G2-P1H11 to the G2 positional variant-HLAs, indicating positions 3-9 of the restricted peptide as likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone. 
       FIG. 91A  depicts stability results from a second experiment for the G2 positional variant-HLAs, further confirming that positions 2, 3, and 9 were not amenable to amino acid substitutions and therefore are likely to be important for the peptide to bind the HLA protein. 
       FIG. 91B  depicts binding affinity of Fab clone G2-P2C06 to the G2 positional variant-HLAs, indicating positions 7-8 of the restricted peptide as likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone. 
       FIG. 74A  depicts stability results for the G7 positional variant-HLAs, indicating that positions 1, 2, 6, and 9 were not amenable to amino acid substitutions and therefore are likely to be important for the peptide to bind the HLA protein. 
       FIG. 74B  depicts binding affinity of Fab clone G7R4-B5-P2E9 to the G7 positional variant-HLAs, indicating positions 1-5 of the restricted peptide as likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone. 
     In a second positional scanning experiment, libraries of variant restricted peptides for each HLA-PEPTIDE target were generated, wherein each variant differed from the corresponding HLA-PEPTIDE target by 1-3 amino acids. Binding affinity of the selected Fab clone to the variant peptide-HLA complexes was assessed by BLI as described above. Variants that weakened Fab binding may identify residues that are likely involved, directly or indirectly, in determining the interaction of the peptide-HLA complex with the Fab clone 
     Data from the second positional scanning experiment, assessing binding capability of G2P1H11 ABP to the library of A*01:01_NTDNNLAVY variants, revealed that several single or multiple variants at positions 4-9 were able to eliminate detectable binding by biolayer interferometry (BLI) (data not shown). These data were largely consistent with the first positional scanning experiment described above. 
     Data from the second positional scanning experiment, assessing binding capability of G10P3E04 ABP to the library of A*01:01_ASSLPTTMNY variants, revealed that variant peptides which failed to bind G10P3E04 comprised 2 amino acid differences compared to the target peptide across positions 6-9. These data support the notion that peptide positions 6-9 are important directly or conformationally for binding to this target. 
     Example 6: Antibodies Bind Cells Presenting HLA-PEPTIDE Target Antigens HLA-B*35:01 EVDPIGHVY, HLA-A*02:01 AIFPGAVPAA, and HLA-A*01:01 ASSLPTTMNY 
     To verify that the identified TCR-like antibodies bind their pHLA target G5, G8 and G10 in their natural context, e.g., on the surface of antigen-presenting cells, selected clones were reformatted to IgG and used in binding experiments with K562 cells expressing the cognate HLA-PEPTIDE target. Briefly, cells were transduced with either HLA-B*35:01 for the G5 target peptide, HLA-A*02:01 for the G7 and G8 target peptides, or HLA-A*01:01 for the G2 and G10 target peptides. The cells were then exogenously pulsed with target or negative control peptide, e.g., as specified in Tables 1 and 2, using established methods to generate the relevant pHLA complexes on the cell surface. 
     Materials and Methods 
     Retroviral Production 
     The Phoenix-AMPHO cells (ATCC®, CRL-3213™) were cultured in DMEM (Corning™, 17-205-CV) supplemented with 10% FBS (Seradigm, 97068-091) and Glutamax (Gibco™, 35050079). K-562 cells (ATCC®, CRL-243™) were cultured in IMDM (Gibco™, 31980097) supplemented with 10% FBS. Lipofectamine LTX PLUS (Fisher Scientific, 15338100) contains a Lipofectamine reagent and a PLUS reagent. Opti-MEM (Gibco™, 31985062) was purchased from Fisher Scientific. 
     Phoenix cells were plated at 5×10 5  cells/well in a 6 well plate and incubated overnight at 37° C. For the transfection, 10 μg plasmid, 10 μL Plus reagent and 100 μL Opti-MEM were incubated at room temperature for 15 minutes. Simultaneously, 8 μL Lipofectamine was incubated with 92 μL Opti-MEM at room temperature for 15 minutes. These two reactions were combined and incubated again for 15 minutes at room temperature after which 800 μL Opti-MEM was added. The culture media was aspirated from the Phoenix cells and they were washed with 5 mL pre-warmed Opti-MEM. The Opti-MEM was aspirated from the cells and the lipofectamine mixture was added. The cells were incubated for 3 hours at 37° C. and 3 mL complete culture medium was added. The plate was then incubated overnight at 37° C. The media was replaced with Phoenix culture medium and the plate incubated an additional 2 days at 37° C. 
     The media was collected and filtered through a 45 μm filter into a clean 6 well dish. 20 μL Plus reagent was added to each virus suspension and incubated at room temperature for 15 minutes followed by the addition of 8 μL/well of Lipofectamine and another 15 min room temperature incubation. 
     K562 Cell Line Generation (Retroviral Transduction with HLA) 
     K562 cells were counted and resuspended to 5E6 cells/mL and 100 μL added to each virus suspension. The 6 well plate was centrifuged at 700 g for 30 minutes and then incubated at 37° C. for 5-6 hours. The cells and virus suspension were then transferred to a T25 flask and 7 mL K562 culture medium was added. The cells were then incubated for three days. The transduced K562 cells were then cultured in medium supplemented with 0.6 μg/mL Puromycin (Invivogen, ant-pr-1) and selection monitored by flow cytometry. 
     Flow Cytometry Methods: 
     HLA-transduced K562 cells were pulsed the night before with 50 μM of peptide (Genscript) in IDMEM containing 1% FBS in 6 well plates and incubated under standard tissue culture conditions. Cells were harvested, washed in PBS, and stained with eBioscience Fixable Viability Dye eFluor 450 for 15 minutes at room temperature. Following another wash in PBS+1 −2 % FBS, cells were resuspended with IgGs at varying concentrations. Cells were incubated with antibodies for 1 hour at 4° C. After another wash, PE-conjugated goat anti-human IgG secondary antibody (Jackson ImmunoResearch) was added at 1:100 to 1:200 for 30 minutes at 4° C. After washing in PBS+1-2% FBS, cells were resuspended in PBS+1-2% FBS and analyzed by flow cytometry. Flow cytometric analysis was performed on the Attune NxT Flow Cytometer (ThermoFisher) using the Attune NxT Software. Data were analyzed using FlowJo. 
     Results 
     Four representative examples of antibody binding to either G5-, G8- or G10-presenting K562 cells, as detected by flow cytometry, are shown in  FIGS. 17A, 17B, and 17C . Antibody binding was observed in a dose-dependent manner that was selective for the relevant target peptides. 
     In another flow cytometry experiment, HLA-transduced K562 cells were pulsed with 50 μM of target or control peptides as listed in Table 1 for G5 and in Table 2 for G8 and G10, and pHLA-specific antibodies were detected by flow cytometry. HLA-transduced K562 cells were pulsed with 50 μM of target or negative control peptides and antibody binding histograms were plotted for G5-P7A05 at 20 μg/mL, G8-2C10 at 30 μg/mL, G10-P1B07 at 30 μg/mL, and G8-P1C11 at 30 μg/mL. Histograms are depicted in  FIG. 18  and  FIG. 47 . 
     Results are shown in  FIGS. 75 and 76 . Both G2-P1H11 and G7R4-B5-P2E9 selectively bound HLA-transduced K562 cells pulsed with the target peptide, as compared to HLA-transduced cells pulsed with the negative control peptides. 
     In another flow cytometry experiment, HLA-B*35:01-transduced K562 cells were pulsed with 50 μM of target peptide EVDPIGHVY (“EVD”) or negative control peptide IPSINVHHY (“IPS”), and pHLA-specific antibodies were detected by flow cytometry. Results for G5 antibodies G5-7A05 and G4-1C12 are shown in  FIG. 102 . Antibody binding was observed at all doses in a manner that was selective for the target peptide. 
     In another flow cytometry experiment, HLA-A*02:01-transduced K562 cells were pulsed with 50 μM of target peptide AIFPGAVPAA (“AIF”) or negative control peptide FLLTRILTI (“FLL”), and pHLA-specific antibodies were detected by flow cytometry. Results for G8 specific antibodies G8-1B03, G8-5G08, G8-4F05, G81C11, G82C10, and G82C11 are shown in  FIG. 103 . Antibody binding was observed at all doses in a manner that was selective for the target peptide. 
     In another flow cytometry experiment, HLA-A*01:01-transduced K562 cells were pulsed with 50 μM of target peptide ASSLPTTMNY (“ASSL”) or negative control peptide ATDALMTGY (“ATDA”), and pHLA-specific antibodies were detected by flow cytometry. Results for G10 specific antibodies G10-3E09 and G10-1H01 are shown in  FIG. 104 . Antibody binding was observed in a dose dependent manner in a manner that was selective for the target peptide. 
     Example 7: Antibodies Bind to Tumor Cell Lines that Express the Target Gene and HLA Subtype 
     Tumor cell lines were chosen based on expression of the HLA subtype and target gene of interest, as assessed by a publicly available database (TRON http://celllines.tron-mainz.de). The selection of the tumor cell line for cell binding assays is shown in Table 12 below. 
     
       
         
           
               
             
               
                 TABLE 12 
               
             
            
               
                   
               
               
                 selection of tumor cell lines for cell binding assay 
               
            
           
           
               
               
               
            
               
                 Cell line 
                 Target expression 
                 HLA type 
               
               
                   
               
               
                 LN229  
                 MAGEA6 (137.6 RPKM) 
                 B*35:01; B*35:01 (26.53 RPKM) 
               
               
                 (G5) 
                   
                   
               
               
                 BV173  
                 FOXE1 (18.1 RPKM) 
                 A*30:01; A*02:01 (142.25 RPKM) 
               
               
                 (G8) 
                   
                   
               
               
                 Colo829 
                 MAGEA3 (119.3 RPKM) 
                 A*01:01; A*0101 (143.7 RPKM) 
               
               
                 (G10) 
                 MAGEA6 (215.4 RPKM) 
               
               
                   
               
            
           
         
       
     
     The LN229, BV173, and Colo829 tumor cell lines were propagated under standard tissue culture conditions. Flow cytometry was performed as described in Example 6. Cells were incubated with 30 μg/mL or 0 μg/mL antibody followed by PE conjugated anti-human secondary IgG. 
     Results are depicted in  FIG. 19 . Panel A shows a histogram plot for G5-P7A05 binding to glioblastoma line LN229. Panel B shows a histogram plot for G8-P2C10 binding to leukemia line BV173. Panel C shows a histogram plot for G10-P1B07 binding to CRC line Colo829. 
     Example 8: Identification of TCRs that Bind HLA-PEPTIDE Target HLA-A*01:01 ASSLPTTMNY or HLA-PEPTIDE Target HLA-A*01:01 HSEVGLPVY 
     Peripheral blood mononuclear cells (PBMCs) were obtained by processing leukapheresis samples from healthy donors. Frozen PBMCs were thawed and incubated with cocktail of biotinylated CD45RO, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD57, CD123, anti-HLA-DR, CD235a (Glycophorin A), CD244, and CD4 antibodies and were subsequently magnetically labeled with anti-biotin microbeads for removal from PBMC population. Enriched naïve CD8 T cells were labelled with tetramers comprising target peptide and appropriate MHC molecule, stained with live/dead and lineage markers and sorted by flow cytometry cell sorter. Following polyclonal expansion, one of two paths may be taken. If a large fraction of population is specific for the HLA-PEPTIDE target, the T cell population may be sequenced as a whole. Alternatively, the cells harboring TCRs specific for the HLA-PEPTIDE target may be resorted, and only cells isolated after resort are sequenced using 10× Genomics single cell resolution paired immune TCR profiling approach. 
     Here, cells harboring TCRs specific for the HLA-PEPTIDE target HLA-A*01:01 ASSLPTTMNY were resorted and sequenced as described above. Specifically, two-to-eight thousand live T cells were partitioned into single cell emulsions for subsequent single cell cDNA generation and full-length TCR profiling (5′ UTR through constant region—ensuring alpha and beta pairing). This approach utilized a molecularly barcoded template switching oligo at the 5′ end of the transcript. An alternative approach utilizes a molecularly barcoded constant region oligo at the 3′ end. Another alternative approach couples an RNA polymerase promoter to either the 5′ or 3′ end of a TCR. All of these approaches enable the identification and deconvolution of alpha and beta TCR pairs at the single-cell level. The resulting barcoded cDNA transcripts underwent an optimized enzymatic and library construction workflow to reduce bias and ensure accurate representation of clonotypes within the pool of cells. Libraries were sequenced on Illumina&#39;s MiSeq or HiSeq4000 instruments (paired-end 150 cycles) for a target sequencing depth of about five to fifty thousand reads per cell. 
     Sequencing reads were processed through the 10× provided software Cell Ranger. Sequencing reads are tagged with a Chromium cellular barcodes and UMIs, which are used to assemble the V(D)J transcripts cell by cell. The assembled contigs for each cell were then annotated by mapping the assembled contigs to the Ensemble v87 V(D)J reference sequences. Clonotypes were defined as alpha, beta chain pairs of unique CDR3 amino acid sequences. Clonotypes were filtered for single alpha and single beta chain pairs present at frequency above 2 cells to yield the final list of clonotypes per target peptide in a specific donor. 
     Two different donors were analyzed over 6 experiments for ASSLPTTMNY and 2 experiments for HSEVGLPVY targets.  FIGS. 20A and 20B  show the number of target-specific T cells isolated per experiment and number of target-specific unique clonotypes identified per experiment, respectively. Each color represent data from one experiment. 
     Table 13 depicts the cumulative number of T cells and unique TCRs identified across all experiments and average number of target-specific T cells per 3 million of naïve CD8 T cells. 
     
       
         
           
               
             
               
                 TABLE 13 
               
             
            
               
                   
               
               
                 cumulative data from TCR identification experiment 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 Cumu- 
                 Average  
                 Cumu- 
               
               
                   
                   
                   
                 lative 
                 fre- 
                 lative 
               
               
                   
                   
                   
                 num- 
                 quency 
                 number 
               
               
                   
                   
                   
                 ber 
                 per 3E6  
                 of 
               
               
                   
                   
                   
                 of  
                 naïve  
                 identified 
               
               
                 Target  
                   
                 HLA 
                 isolated 
                 CD8 T 
                 clono- 
               
               
                 sequence 
                 Gene 
                 restriction  
                 cells 
                 cells 
                 types 
               
               
                   
               
               
                 ASSLPTTMNY 
                 MAGE 
                 A*01:01 
                 3516 
                 464 
                 550 
               
               
                   
                 A3/6 
                   
                   
                   
                   
               
               
                 HSEVGLPVY 
                 DCAF12L1 
                 A*01:01 
                 1762 
                 539 
                 142 
               
               
                   
               
            
           
         
       
     
     Annotated sequences of the identified TCR clonotypes specific for HLA-PEPTIDE A*01:01_ASSLPTTMNY are shown in Table 14. This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     Alpha and beta CDR3 sequences of the identified TCR clonotypes specific for HLA-PEPTIDE A*01:01_ASSLPTTMNY are shown in Table 15. For clarity, as in Table 14, each identified TCR was assigned a TCR ID number. For example TCR ID #1 comprises the αCDR3 sequence CAGPGNTGKLIF and the βCDR3 sequence CASSNAGDQPQHF. 
     Full length alpha V(J) and beta V(D)J sequences of the identified TCR clonotypes specific for HLA-PEPTIDE A*01:01_ASSLPTTMNY are shown in Table 16. For example TCR ID #1 comprises the alpha V(J) sequence MLLITSMLVLWMQLSQVNGQQVMQIPQYQHVQEGEDFTTYCNSSTTLSNIQWYKQ RPGGHPVFLIQLVKSGEVKKQKRLTFQFGEAKKNSSLHITATQTTDVGTYFCAGPGN TGKLIFGQGTTLQVK and the beta V(D)J sequence 
     
       
         
           
               
            
               
                 MSNQVLCCVVLCFLGANTVDGGITQSPKYLFRKEGQNVTLSCEQNLNHDA 
               
               
                 MYWYRQDPGQGLRLIYYSQIVNDFQKGDIAEGYSVSREKKESFPLTVTSA 
               
               
                 QKNPTAFYLCASSNAGDQPQHFGDGTRLSIL. 
               
            
           
         
       
     
     Annotated sequences of the identified TCR clonotypes specific for HLA-PEPTIDE A*01:01_HSEVGLPVY are shown in Table 17. This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     Alpha and beta CDR3 sequences of the identified TCR clonotypes specific for HLA-PEPTIDE A*01:01 HSEVGLPVY are shown in Table 18. This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     Full length alpha V(J) and beta V(D)J sequences of the identified TCR clonotypes specific for HLA-PEPTIDE A*01:01 HSEVGLPVY are shown in Table 19. This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     Example 9: Identification of Antibodies or Antigen-Binding Fragments Thereof that Bind HLA-PEPTIDE Complexes 
     Identification of Single-Chain Variable Fragment (scFv) Antibodies Targeting MHC Class I Molecules Presenting Tumor Antigens 
     Potent and selective single chain antibodies targeting human class I MHC molecules presenting tumor antigens of interest are identified using phage display. Phage libraries are prepared for screening by removing non-specific class I MHC binders. Multiple soluble human peptide-MHC (pMHC) molecules different from the target pMHCs are utilized to pan pre-existing phage libraries to remove scFvs that non-specifically bind class I MHC. To identify scFvs that selectively bind pMHCs of interest, target pMHCs are utilized for at least 1-3 rounds of panning with the prepared phage library. scFv hits identified in the screen are then evaluated against a panel of irrelevant pMHCs to identify scFv leads that bind selectively to the target pMHCs. Lead scFvs are characterized to determine target binding specificity and affinity. Lead scFvs that demonstrate potent and selective binding are converted to full-length IgG monoclonal antibody (mAb) constructs. In addition, the lead scFvs are incorporated into bi-specific mAb constructs and chimeric antigen receptor (CAR) constructs that can be used to generate CAR T-cells. Full-length bi-specifics or scFV-based bi-specifics can be constructed. 
     Demonstrate Targeting of Human Tumor Cells In Vitro 
     Immunohistochemistry techniques are utilized to demonstrate specific binding of lead antibodies to human tumor cells or cell lines expressing target pMHC molecules. T-cell lines transfected with CAR-T constructs are incubated with human tumor cells to demonstrate killing of tumor cells in vitro. Alternatively, tumor cells expressing the target are incubated with bi-specific constructs (encoding the ABP and an effector domain) and PBMCs or T cells. 
     In Vivo Proof-of-Concept 
     Lead antibody or CAR-T constructs are evaluated in vivo to demonstrate directed tumor killing in humanized mouse tumor models. Lead antibody or CAR-T constructs are evaluated in xenograft tumor models engrafted with human tumors and PBMCs. Anti-tumor activity is measured and compared to control constructs to demonstrate target-specific tumor killing. 
     Identification of Monoclonal Antibodies (mAbs) that Target MHC Class I Molecules Presenting Tumor Antigens Using Rabbit B Cell Cloning Technologies 
     Potent and selective mAbs targeting human class I MHC molecules presenting tumor antigens of interest are identified. Soluble human pMHC molecules presenting human tumor antigens are utilized for multiple mouse or rabbit immunizations followed by screening of B cells derived from the immunized animals to identify B cells that express mAbs that bind to target class I MHC molecules. Sequences encoding the mAbs identified from the mouse or rabbit screens will be cloned from the isolated B cells. The recovered mAbs are then evaluated against a panel of irrelevant pMHCs to identify lead mAbs that bind selectively to the target pMHCs. Lead mAbs will be fully characterized to determine target binding affinity and selectivity. Lead mAbs that demonstrate potent and selective binding are humanized to generate full-length human IgG monoclonal antibody (mAb) constructs. In addition, the lead mAbs are incorporated into bi-specific mAb constructs and chimeric antigen receptor (CAR) constructs that can be used to generate CAR T-cells. Full-length bi-specifics or scFV-based bi-specifics can be constructed. 
     Demonstrate Targeting of Human Tumor Cells In Vitro 
     Immunohistochemistry techniques are utilized to demonstrate specific binding of lead antibodies to human tumor cells expressing target pMHC molecules. T-cell lines transfected with CAR-T constructs are incubated with human tumor cells to demonstrate killing of tumor cells in vitro. Alternatively, tumor cells expressing the target are incubated with bi-specific constructs (encoding the ABP and an effector domain) and PBMCs or T cells. 
     In Vivo Proof-of-Concept 
     Lead antibody or CAR-T constructs are evaluated in vivo to demonstrate directed tumor killing in humanized mouse tumor models. Lead antibody or CAR-T constructs are evaluated in xenograft tumor models engrafted with human PBMCs. Anti-tumor activity is measured and compared to control constructs to demonstrate target-dependent tumor killing. 
     Potent and selective ABPs that selectively target human class I MHC molecules presenting tumor antigens will be identified using phage display or B cell cloning technologies. The utility of the ABPs will be demonstrated by showing that the ABPs mediated tumor cell killing in vitro and in vivo when incorporated into antibody or CAR-T cell constructs. 
     Example 10: Identification of TCRs that Bind HLA-PEPTIDE Complexes 
     To select natural high affinity TCRs, specifically recognizing shared antigen MHC/peptide targets (SAT), the following experimental steps are taken: 
     1. Identification and isolation of MHC/peptide target-reactive TCRs 
     2. Production of engineered TCR T cells 
     3. Verification of TCR specificity 
     Identification of MHC/Peptide Target-Reactive TCRs 
     T cells are isolated from blood, lymph nodes, or tumors of patients. Patients are HLA-matched to SAT, and are selected based on expression of target-harboring protein. T cells are then enriched for SAT-specific T cells, e.g., by sorting SAT-MHC tetramer binding cells or by sorting activated cells stimulated in an in vitro co-culture of T cells and SAT-pulsed antigen presenting cells. 
     SAT-relevant alpha-beta TCR dimers are identified by single cell sequencing of TCRs of SAT-specific T cells. Alternatively, bulk TCR sequencing of SAT-specific T cells is performed and alpha-beta pairs with a high probability of matching are determined using a TCR pairing method. 
     Alternatively or in addition, SAT-specific T cells can be obtained through in vitro priming of naïve T cells from healthy donors. T cells obtained from PBMCs, lymph nodes, or cord blood are repeatedly stimulated by SAT-pulsed antigen presenting cells to prime differentiation of antigen-experienced T cells. TCRs are then identified similarly as described above for SAT-specific T cells from patients. 
     Production of Engineered TCR T Cells 
     TCR alpha and beta chain sequences are cloned into appropriate constructs. TCR-autologous or heterologous bulk T cells are transduced with the constructs to produce engineered TCR T cells. These T cells are expanded in the presence of anti-CD3 antibodies and IL-2 cytokine for use in subsequent experiments. In certain instances, native TCR is deleted or the inserted TCR is modified to increase proper multimerization. 
     In Vitro Verification of TCR Specificity 
     First, T cells bearing engineered TCRs are screened for target recognition using antigen presenting cells expressing the appropriate MHC and pulsed with appropriate target(s). 
     TCRs identified in the first round of screening are then tested for recognition of natural target. Lead TCRs are nominated based on specific recognition of HLA-matched primary tumors and tumor cell lines expressing SAT-harboring protein. 
     To assure specificity, lead TCRs are de-selected based on off-target recognition. They are screened against a panel of HLA matched and mismatched cell lines, covering multiple tissues and organ types, and with HLA-matched and mismatched antigen presenting cells pulsed with a panel of infectious disease antigens. TCRs with specific and non-specific off-target recognition of self-antigens or common non-self-antigens are de-selected. 
     Example 11: Identification of MHC/Peptide Target-Reactive TCRs 
     T cells are isolated from blood, lymph nodes, or tumors of patients. Patients are HLA-matched to SAT, and are selected based on expression of target-harboring protein. T cells are then enriched for SAT-specific T cells, e.g., by sorting SAT-MHC tetramer binding cells or by sorting activated cells stimulated in an in vitro co-culture of T cells and SAT-pulsed antigen presenting cells. 
     SAT-relevant alpha-beta TCR dimers are identified by single cell sequencing of TCRs of SAT-specific T cells. Alternatively, bulk TCR sequencing of SAT-specific T cells is performed and alpha-beta pairs with a high probability of matching are determined using a TCR pairing method. 
     Alternatively or in addition, SAT-specific T cells can be obtained through in vitro priming of naïve T cells from healthy donors. T cells obtained from PBMCs, lymph nodes, or cord blood are repeatedly stimulated by SAT-pulsed antigen presenting cells to prime differentiation of antigen-experienced T cells. TCRs are then identified similarly as described above for SAT-specific T cells from patients. 
     Example 12: Production of Engineered TCR T Cells 
     TCR alpha and beta chain sequences are cloned into appropriate constructs. TCR-autologous or heterologous bulk T cells are transduced with the constructs to produce engineered TCR T cells. These T cells are expanded in the presence of anti-CD3 antibodies and IL-2 cytokine for use in subsequent experiments. In certain instances, native TCR is deleted or the inserted TCR is modified to increase proper multimerization. 
     In Vitro Verification of TCR Specificity 
     First, T cells bearing engineered TCRs are screened for target recognition using antigen presenting cells expressing the appropriate MHC and pulsed with appropriate target(s). 
     TCRs identified in the first round of screening are then tested for recognition of natural target. Lead TCRs are nominated based on specific recognition of HLA-matched primary tumors and tumor cell lines expressing SAT-harboring protein. 
     To assure specificity, lead TCRs are de-selected based on off-target recognition. They are screened against a panel of HLA matched and mismatched cell lines, covering multiple tissues and organ types, and with HLA-matched and mismatched antigen presenting cells pulsed with a panel of infectious disease antigens. TCRs with specific and non-specific off-target recognition of self-antigens or common non-self-antigens are de-selected. 
     Example 13: Identification of Monoclonal Antibodies (mAbs) that Target MHC Class I Molecules Presenting Tumor Antigens Using Rabbit B Cell Cloning Technologies 
     Potent and selective mAbs targeting human class I MHC molecules presenting tumor antigens of interest are identified. Soluble human pMHC molecules presenting human tumor antigens are utilized for multiple mouse or rabbit immunizations followed by screening of B cells derived from the immunized animals to identify B cells that express mAbs that bind to target class I MHC molecules. Sequences encoding the mAbs identified from the mouse or rabbit screens will be cloned from the isolated B cells. The recovered mAbs are then evaluated against a panel of irrelevant pMHCs to identify lead mAbs that bind selectively to the target pMHCs. Lead mAbs will be fully characterized to determine target binding affinity and selectivity. Lead mAbs that demonstrate potent and selective binding are humanized to generate full-length human IgG monoclonal antibody (mAb) constructs. In addition, the lead mAbs are incorporated into bi-specific mAb constructs and chimeric antigen receptor (CAR) constructs that can be used to generate CAR T-cells. Full-length bi-specifics or scFV-based bi-specifics can be constructed. 
     Demonstrate Targeting of Human Tumor Cells In Vitro 
     Immunohistochemistry techniques are utilized to demonstrate specific binding of lead antibodies to human tumor cells expressing target pMHC molecules. T-cell lines transfected with CAR-T constructs are incubated with human tumor cells to demonstrate killing of tumor cells in vitro. Alternatively, tumor cells expressing the target are incubated with bi-specific constructs (encoding the ABP and an effector domain) and PBMCs or T cells. 
     In Vivo Proof-of-Concept 
     Lead antibody or CAR-T constructs are evaluated in vivo to demonstrate directed tumor killing in humanized mouse tumor models. Lead antibody or CAR-T constructs are evaluated in xenograft tumor models engrafted with human PBMCs. Anti-tumor activity is measured and compared to control constructs to demonstrate target-dependent tumor killing. 
     Potent and selective ABPs that selectively target human class I MHC molecules presenting tumor antigens will be identified using phage display or B cell cloning technologies. The utility of the ABPs will be demonstrated by showing that the ABPs mediated tumor cell killing in vitro and in vivo when incorporated into antibody or CAR-T cell constructs. 
     Example 14: Assessment of scFv-pHLA or Fab-pHLA Structures by Hydrogen/Deuterium Exchange and Mass Spectrometry 
     Experimental Procedures 
     Hydrogen/Deuterium Exchange. 
     20 μM of HLA-peptide was incubated with a ˜3-fold molar excess of scFv or Fab formatted proteins for 20 min at room temperature (20-25° C.) to generate complexes for the exchange experiments. For the Apo (unbound) control, the HLA-peptide was incubated with an equal volume of 50 mM NaCl, 20 mM Tris pH 8.0. All subsequent reaction steps were performed at 4° C. by an automated HDX PAL system controlled by Chronos 4.8.0 software (Leap Technologies, Morrisville, N.C.). 5 μl of protein complexes were diluted 10-fold into H20 or 50 mM NaCl, 20 mM Tris pH 8.0 (for the 0 min. control time-point) or the same buffer made with D20 for 30s prior to quenching in 0.8 M guanidine hydrochloride, 0.4% acetic acid (v/v), and 75 mM tris(2-carboxyethyl) phosphine for 3 min. ˜50 pmol of quenched protein complexes were transferred onto an immobilized Protein XIII/Pepsin column (NovaBioAssays, Woburn, Mass.) for integrated on-line protein digestion. 
     Liquid Chromatography, Mass Spectrometry, and HDX Analysis 
     Chromatographic separation of peptides was carried out using an UltiMate 3000 Basic Manual UHPLC System (ThermoFisher Scientific, Waltham, Mass.), which contained a trap C18 column (5 μM particle size and 2.1 mm diameter) and an analytical C18 column (1.9 μM particle size and 1 mm diameter). Samples were desalted with 10% acetonitrile, 0.05% trifluoroacetic acid or 10% acetonitrile, 0.5% formic acid at a 40 μl/min flow rate for 2 min and peptides were eluted at a 40 μl/min flow rate with an increasing concentration gradient of 95% acetonitrile with trifluoro acetic acid or formic acid. Mass spectrometry was performed with an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher, Waltham, Mass.) with the ESI source set at a positive ion voltage of 3500-3800 V. Prior to performing hydrogen-deuterium exchange experiments, peptide fragments of each HLA-peptide complex were analyzed by data-dependent LC/MS/MS and the data searched using PEAKS Studio (Bioinformatics Solutions Inc., Waterloo, ON, Canada) with a peptide precursor mass tolerance of 20 ppm and fragment ion mass tolerance of 0.2 Da. The HLA, 02M, and target peptide sequences were searched, and false detection rates identified using a decoy-database strategy. Peptides from the hydrogen-deuterium experiments were detected by LC/MS and analyzed by HDX Workbench (Omics Informatics, Honolulu, Hi.) with a retention time window size of 0.22 min and a 7.0 ppm error tolerance. High-resolution HD exchange data for selected peptides were obtained by fragmenting the peptides by Electron Transfer Dissociation (ETD) with a reaction time of 200 ms (G2) or 100 ms (G10), using fluoranthene as the reagent anion. Peptide fragments were analyzed by HDExaminer (Sierra Analytics) with a retention time window size of 18s and a peptide m/z tolerance of 2 Da. Heat maps of deuterium uptake differences were generated by Microsoft Excel and mapped on to relevant protein crystallographic structures using Pymol (Schrödinger, Cambridge, Mass.). 
     For the results below, amino acid numbering of the HLA alpha helices is based on literal numbering of the mature protein, based on the following: (1) removal of signal peptide, and (2) addition of N-terminal methionine for bacterial expression. The HLA subtype amino acid reference sequences and the beta-2 microglobulin amino acid sequence are provided in Table 38. 
     Results 
       FIG. 21A  shows an exemplary heatmap of the HLA portion of the G8 HLA-PEPTIDE complex when incubated with scFv clone G8-P1H08, visualized in its entirety using a consolidated perturbation view. 
       FIG. 98  shows an example of high resolution data from scFv clone G5-P1C12 plotted on crystal structure of HLA-B*35:01 (5xos.pdb; https://www.rcsb.org/structure/5XOS). 
     An example of the data from scFv G8-P1H08 plotted on the crystal structure 1jf1.pdb, available at http://www.rcsb.org/structure/1JF1, is shown in  FIG. 21B . 
     An example of high-resolution HDX data from scFv G8-P1H08 plotted on a crystal structure of Fab clone G8-P1C11 complexed with HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”), is shown in  FIG. 101 . 
       FIG. 45A  shows an exemplary heatmap of the HLA portion of the G8 HLA-PEPTIDE complex when incubated with scFv clone G8-P1C11 (structure shown in  FIG. 45B ), visualized in its entirety using a consolidated perturbation view. 
     An example of the data from scFv G8-P1C11 plotted on the crystal structure described in Example 15 is shown in  FIG. 45B . 
       FIG. 23A  shows an exemplary heatmap of the HLA portion of the G10 HLA-PEPTIDE complex when incubated with scFv clone R3G10-P2G11, visualized in its entirety using a consolidated perturbation view. 
     An example of the data from scFv R3G10-P2G11 plotted on a crystal structure PDB5bs0 is shown in  FIG. 23B . The crystal structure, depicting a restricted peptide in the HLA binding cleft formed by the α1 and α2 helices, can be found at URL https://www.rcsb.org/structure/5bs0 (Raman et al). 
     An example of data from a second round of HDX studies, from scFv-G10-P5A08, plotted on a crystal structure 5bs0.pdb is shown in  FIG. 23C . The crystal structure, depicting a restricted peptide in the HLA binding cleft formed by the α1 and α2 helices, can be found at URL https://www.rcsb.org/structure/5bs0 (Raman et al). 
     To better compare the data across the ABPs tested for a given HLA-PEPTIDE target, data for each ABP was exported, and a heat map was generated in Excel.  FIG. 22A  shows resulting heat maps from a first round of HDX experiments across the HLA α1 helix for all ABPs tested for HLA-PEPTIDE target G8 (HLA-A*02:01_AIFPGAVPAA).  FIG. 22B  shows resulting heat maps across the HLA α2 helix for all ABPs tested for HLA-PEPTIDE target G8 (HLA-A*02:01_AIFPGAVPAA).  FIG. 22C  shows resulting heat maps across the restricted peptide AIFPGAVPAA for all ABPs tested. The heat maps from the first round of HDX data indicate positions 45-60 and 81-84 of the HLA protein (in the α1 helix) of HLA-PEPTIDE target G8 (HLA-A*02:01_AIFPGAVPAA) as likely involved, directly or indirectly, in determining the interaction between the HLA-PEPTIDE target and G8-specific antibody-based ABPs. 
       FIG. 99  shows resulting color heat maps from high resolution HDX experiments across the HLA α1 helix, the HLA α2 helix, and restricted peptide AIFPGAVPAA for all ABPs tested for HLA-PEPTIDE target G8 (HLA-A*02:01_AIFPGAVPAA).  FIG. 100  shows a numerical representation of the color heat maps of  FIG. 99 . The heat maps from the second round of HDX data indicate positions 46, 49, 55, 61, 74, 76, 77, 78, 81 and 84 of the HLA protein (in the α1 helix) as likely involved, directly or indirectly, in determining the interaction between the HLA-PEPTIDE target and G8-specific antibody-based ABPs. The heat maps from the second round of HDX data indicate positions 137, 138, 145, 147, 152-157 of the HLA protein (in the α2 helix) as likely involved, directly or indirectly, in determining the interaction between the HLA-PEPTIDE target and G8-specific antibody-based ABPs. The heat maps from the second round of HDX data indicate positions 5 and 6 of the restricted peptide AIFPGAVPAA as likely involved, directly or indirectly, in determining the interaction between the HLA-PEPTIDE target and G8-specific antibody-based ABPs. 
       FIG. 96  shows resulting color heat maps from high resolution HDX experiments across the HLA α1 helix, the HLA α2 helix, and restricted peptide EVDPIGHVY for all ABPs tested for HLA-PEPTIDE target G5 (HLA-B*35:01_EVDPIGHVY).  FIG. 97  shows a numerical representation of the color heat map of  FIG. 96 . These heat maps indicate positions 50, 54, 55, 57, 61, 62, 74, 81, 82 and 85 of the HLA protein (in the α1 helix) as likely involved, directly or indirectly, in determining the interaction between the HLA-PEPTIDE target and G5-specific antibody-based ABPs. These heat maps indicate positions 147 and 148 of the HLA protein (in the α2 helix) as likely involved, directly or indirectly, in determining the interaction between the HLA-PEPTIDE target and G5-specific antibody-based ABPs. 
       FIG. 24A  shows resulting heat maps from a first round of HDX experiments across the HLA α1 helix for all ABPs tested for HLA-PEPTIDE target G10 (HLA-A*01:01_ASSLPTTMNY).  FIG. 24B  shows resulting heat maps from a first round of HDX experiments across the HLA α2 helix for all ABPs tested for HLA-PEPTIDE target G10 (HLA-A*01:01_ASSLPTTMNY).  FIG. 24C  shows resulting heat maps from a first round of HDX experiments across the restricted peptide ASSLPTTMNY for all ABPs tested.  FIG. 92  shows resulting heat maps from a second round of HDX experiments across the HLA α1 helix, the HLA α2 helix, and the restricted peptide ASSLPTTMNY for all ABPs tested. Taken together, the heat maps indicate positions 49-56 and/or 59-66 of the HLA protein (in the α1 helix), as well as positions 136-147 and 157-160 of the α2 helix of the HLA protein, as likely involved, directly or indirectly, in determining the interaction between the HLA-PEPTIDE target and G10-specific antibody-based ABPs. In particular, all of the ABPs tested decreased solvent accessibility of positions 52-54 of the HLA α1 helix. 
     An example of the data from scFv G2-P1G07 plotted on a crystal structure PDB 5bs0 is shown in  FIG. 77 . The crystal structure can be found at URL https://www.rcsb.org/structure/5bs0 (Raman et al). Areas not covered with MS data are shown in black and those with the greatest decrease in D exchange (indicating a binding site for the ABP) is circled. For clarity, only the binding groove and helices are shown. 
     An exemplary heatmap for scFv clone G2-P1G07 visualized in its entirety using a consolidated perturbation view is shown in  FIG. 78 . 
     An example of the data from scFv G2-P2C11 plotted on a crystal structure PDB 5bs0 is shown in  FIG. 94 . 
       FIG. 95  shows high resolution HDX data plotted on a crystal structure PDB 5bs0. Data for G2 bound to four different scFvs were obtained by fragmenting peptides by Electron Transfer Dissociation (ETD) as described in the Experimental Procedures. 
     To better compare the data across the ABPs tested for a given HLA-PEPTIDE target, data for each ABP was exported, and a heat map was generated in Excel. Resulting heat maps are shown in  FIG. 79  showing a heat map across the α1 helix (top) and across the α2 helix (bottom).  FIG. 80  shows a heat map for all ABPs tested for A*01:01_NTDNNLAVY, across restricted peptide residues 1-9. Heat maps from a second (higher resolution) round of HDX data are shown in  FIG. 93 . Taken together, the heat maps elucidated regions of reduced solvent accessibility in the HLA alpha subunits that bind and display the target peptide. Many of these regions were shared across multiple A*01:01_NTDNNLAVY specific ABPs. The two regions which most commonly exhibited decreased solvent accessibility include A70-Y85 of the alpha 1 helix, and/or positions A140-Y160 of the alpha 2 helix, with all ABPs shielding R157-Y160 of the helix. Taken together, the heat maps also indicate HLA-PEPTIDE/ABP interactions that decrease solvent accessibility across positions 3-9 of the restricted peptide. The effect was increasingly pronounced towards the C-terminal direction. This pattern was consistent for 14 of the 15 antibodies examined, with positions 6-9 invariably being shielded by the presence of the ABPs. All clone entries in the HDX heat maps are scFv formats unless otherwise noted. 
     G7 (A*02:01_LLASSILCA) scFv clones P2E09 and P3A09 were assessed by HDX-MS according to the methods described above. Solvent accessibility was decreased in a region of the HLA-A*02:01 alpha 1 helix corresponding to positions 49-85, with an overlap of G57-K67 (data not shown). Solvent accessibility was also decreased in a region of the alpha 2 helix from positions 136-157, with an overlap between positions 144 and 152. Taken together, these leads cover a broad footprint on HLA. 
     Example 15: Assessment of Fab-PHLA Structures by Crystallography 
     Materials and Methods 
     Complex Purification and Crystal Screening 
     Fab fragments corresponding to, e.g., HLA-PEPTIDE target G8 (A*02:01_AIFPGAVPAA) were concentrated to reach 5 mg/mL (100M) before addition of its corresponding HLA-MHC (1:1 molar ratio) and incubated for 30 minutes at 4° C. The mixture was then injected on size exclusion chromatography column (S200 16/60) equilibrated in 1×PBS buffer for complex purification. Fractions containing both Fab and HLA and with an elution volume consistent with a complex of ˜94 kDa were pooled and concentrated to 10-12 mg/mL (1AU=1 mg/mL). Each purified complex was screened for crystallization conditions using commercial screens: PEGIon (Hampton research), JCSG+(Molecular Dimensions) and JBS Screen 3 and 4 (Jena Biosciences). The choice of the kits was driven by the characteristic of known crystal conditions of HLA-Fab complexes that are mainly based on the use of PEG3350 or PEG4000 as precipitant. 3 to 4 weeks after screen, diffraction suitable crystals appeared for HLA-Fab combinations in several crystallization conditions (Table 24). The protein nature of the crystals was checked by UV. Crystals were transferred into a cryoprotectant solution (crystallization solution supplemented with 25% Glycerol) and flash frozen in liquid nitrogen. 
     Data Collection and Processing 
     Diffraction data was collected on the Proxima 2A beamline at SOLEIL synchrotron (Gif sur Yvette, France). Data processing and scaling was performed using XDS (1). Molecular replacement was performed using MolRep and Arp/Warp from the CCP4 suite (2) using PDB 5E61 for HLA (100% sequence identity) and 5AZE (90% sequence identity with VH) and 5115 (97% sequence identity with VL) for Fab as entry models. Refinement was performed using Buster TNT (GlobalPhasing, Inc) and manual model modifications in Coot (CCP4 suite). 
     Complex Purification 
     Combinations produced a good separation between the individual protein peak and the formed complex peak ( FIG. 28A ). Increasing incubation time to 16 hours (overnight) did not change the ratio of complex formed (˜50% of the protein is present in complex and 50% as free proteins). Peak analysis by SDS PAGE under reducing conditions showed the presence of both Fab chains (30 kDa), HLA heavy chain (˜35 kDa), and HLA light chain (BLM, 10 kDa) in the pooled fractions ( FIG. 28 ). 
     Crystallization and Data Collection 
     Complex pooled fractions were concentrated and screened. After 3-4 weeks crystals appeared for some of the HLA-Fab combinations. A summary of the crystallography conditions for the A*02:01_AIFPGAVPAA-G8-P1C11 Fab complex and resulting crystal formation is shown in Table 24. 
     
       
         
           
               
             
               
                 TABLE 24 
               
             
            
               
                   
               
               
                 Crystallography conditions 
               
            
           
           
               
               
               
            
               
                 Com- 
                   
                 Crystals  
               
               
                 mercial 
                   
                 Obtained  
               
               
                 Kit 
                 Experimental Conditions 
                 (Y/N) 
               
               
                   
               
               
                 JBS 
                 20% PEG4000, 200 mM Magnesium sulfate, 
                 No 
               
               
                   
                 10% glycerol (GOL) 
                   
               
               
                 JBS 
                 20% PEG4000, 200 mM Magnesium sulfate, 
                 Yes 
               
               
                   
                 5% 2-Propanol 
                   
               
               
                 JBS 
                 20% w/v Polyethylene glycol 4,000 10% w/v 
                 No 
               
               
                   
                 2-Propanol, 100 mM HEPES; pH 7.5 
                   
               
               
                 JCSG 
                 20% (w/v) PEG 3350 200 mM Ammonium 
                 No 
               
               
                   
                 chloride 
                   
               
               
                 JCSG 
                 30% (w/v) PEG 2000 MME 100 mM  
                 No 
               
               
                   
                 Potassium thiocyanate 
                   
               
               
                 JCSG 
                 25% (w/v) PEG 3350 100 mM Bis-Tris/ 
                 Yes 
               
               
                   
                 Hydrochloric acid pH 5.5 
                 (integrated  
               
               
                   
                   
                 into P1 
               
               
                   
                   
                 Space group) 
               
               
                 JCSG 
                 30% v/v Jeffamine ® M-600, 0.1M  
                 Yes 
               
               
                   
                 HEPES pH 7.0 
                   
               
               
                 JCSG 
                 25% (w/v) PEG 3350 100 mM Bis-Tris/ 
                 No 
               
               
                   
                 Hydrochloric acid pH 5.5, 200 mM Lithium 
                   
               
               
                   
                 sulfate 
                   
               
               
                 PEGion 
                 0.2M Ammonium tartrate dibasic pH 7.0, 20% 
                 Yes 
               
               
                   
                 w/v Polyethylene glycol 3,350 
                 (integrated  
               
               
                   
                   
                 into P1 
               
               
                   
                   
                 Space group) 
               
               
                 PEGion 
                 2% v/v TacsimateTM pH 6.0 0.1M BIS-TRIS 
                 No 
               
               
                   
                 pH 6.5 20% PEG3350 
                   
               
               
                 PEGion 
                 1% w/v Tryptone 0.001M Sodium azide,  
                 No 
               
               
                   
                 0.05M HEPES sodium pH 7.0, 20% w/v 
                   
               
               
                   
                 Polyethylene glycol 3,350 
               
               
                   
               
            
           
         
       
     
     Out of the tested conditions, four yielded crystals. Two yielded crystals which diffracted well (1.7 to 2.0 Å resolution) and were integrated into a P1 space group (Table 24). Structure resolution was possible by combining molecular replacement (MolRep) and software automated model building using Arp/Warp. 
     An exemplary crystal of a complex comprising Fab clone G8-P1C11 and HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”) is shown in  FIG. 29 . This crystal was grown using the commercial screen JCSG, using 25% (w/v) PEG 3350 100 mM Bis-Tris/Hydrochloric acid pH 5.5. This crystal was used to generate the structural data below. 
     Structural Analysis 
     The overall structure of a complex formed by binding of Fab clone G8-P1C11 to HLA-PEPTIDE target A*02:01_AIFPGAVPAA (“G8”) is shown in  FIG. 30 . The individual proteins are represented as surfaces. The interface area between the HLA and the VH and VL is 747 Å 2  and 285 Å 2 , respectively. 
     During refinement electron density region corresponding to the peptide was clearly visible and allowed peptide side chain unambiguous positioning ( FIG. 31 ) with the provided 10 residue peptide sequence AIFPGAVPAA. All areas relevant to interaction interfaces are refined; however, some refinement is still required in antibody constant regions. 
     Coding of monomers in the complex, which is referred to in the following data, is provided in Table 25 below. 
     
       
         
           
               
             
               
                 TABLE 25 
               
             
            
               
                   
               
               
                 monomer coding used in crystal analysis 
               
            
           
           
               
               
               
            
               
                   
                   
                 Monomer  
               
               
                   
                 Monomer 
                 Code (ID) 
               
               
                   
                   
               
               
                   
                 HLA heavy chain (α1, α2, α3) 
                 A 
               
               
                   
                 HLA β2 microglobulin (light chain) 
                 B 
               
               
                   
                 Restricted peptide 
                 I 
               
               
                   
                 Fab heavy chain (VH-CH1) 
                 C 
               
               
                   
                 Fab light chain (VL-CL) 
                 D 
               
               
                   
                   
               
            
           
         
       
     
     HLA-Peptide Interaction 
     The restricted peptide AIFPGAVPAA is mainly buried in the HLA A*02:01 binding pocket with the residues P4G5A6 protruding towards the Fab. The interaction surface between the peptide and the HLA is 926 Å 2  and represents 76% of the total peptide solvent accessible surface (1215 Å 2 ). The binding of the peptide to the HLA involves 9 hydrogen bonds and van der Waals interactions ( FIG. 32 ) and yields a binding energy of −16.4 kcal/mol. 
     A list of hydrogen interactions is shown in Table 26, below. 
     
       
         
           
               
             
               
                 TABLE 26 
               
             
            
               
                   
               
               
                 Hydrogen bond interactions between restricted peptide and HLA. 
               
            
           
           
               
               
               
               
            
               
                   
                   
                 Distance 
                   
               
               
                   
                 Peptide 
                 (Angstroms) 
                 HLA 
               
               
                   
                   
               
               
                   
                 I:ALA 1[ N ] 
                 2.72 
                 A:TYR 172[ OH ] 
               
               
                   
                 I:ALA 1[ N ] 
                 2.86 
                 A:TYR 8[ OH ] 
               
               
                   
                 I:ILE 2[ N ] 
                 2.81 
                 A:GLU 64[ OE1 ] 
               
               
                   
                 I:ILE 2[ N ] 
                 3.71 
                 A:TYR 8[ OH ] 
               
               
                   
                 I:PHE 3[ N ] 
                 2.94 
                 A:TYR 100[ OH ] 
               
               
                   
                 I:ALA 1[ O ] 
                 2.67 ] 
                 A:TYR 160[ OH 
               
               
                   
                 I:PRO 8[ O ] 
                 2.93 
                 A:ARG 98[ NH2 ] 
               
               
                   
                 I:PRO 8[ O ] 
                 2.89 
                 A:ARG 98[ NH1 ] 
               
               
                   
                 I:ALA 9[ O ] 
                 2.71 
                 A:TRP 148[ NE1 ] 
               
               
                   
                 I:ALA 1[ N ] 
                 2.72 
                 A:TYR 172[ OH ] 
               
               
                   
                   
               
            
           
         
       
     
     A complete interface summary of the HLA and restricted peptide is shown in  FIG. 37 . 
     A complete list of the interacting residues from the restricted peptide and HLA is shown in  FIG. 38 . 
     Fab-Restricted Peptide Interactions 
     As most of the peptide is buried in the binding pocket of the HLA, only part of it available for interactions with the Fab chains. This is confirmed by the observation that 76% of the solvent accessible area of the peptide is occupied by its interaction with the HLA. Interaction surface between the peptide and the heavy chain and the light chain of the Fab is 114.3 and 113.9 Å 2  respectively. This corresponds to 18% of the total peptide solvent accessible area. PISA analysis showed that only two hydrogen bonds are involved in the interaction between the Fab and the peptide: hydroxyl group of Tyr32 from the light chain interacts with the backbone carbonyl of Gly5 of the peptide and the Tyr100A backbone amide interacting with the backbone carbonyl group of Pro4 of the peptide (See Table 27 for a list of the hydrogen interactions, below). 
     
       
         
           
               
             
               
                 TABLE 27 
               
             
            
               
                   
               
               
                 Fab/restricted peptide H bond interactions 
               
            
           
           
               
               
               
            
               
                 Peptide 
                 Distance (A) 
                 Fab 
               
               
                   
               
               
                 I:PRO 4[ O ] 
                 3.0 
                 C:TYR 100A[ OH ] 
               
               
                   
                   
                 (VH) 
               
               
                 I:GLY 5[ O ] 
                 3.7 
                 D:TYR 32[ OH ] (VL) 
               
               
                   
               
            
           
         
       
     
     The recognition mode of the Fab towards the restricted peptide is mainly through hydrophobic interactions and hydrogen bonds involving solvent molecules ( FIGS. 33 and 34 ). The binding energy of the interaction between the Fab and restricted peptide is −2.0 and −1.9 kcal/mol with the VH and VL chains respectively. 
     A complete interface summary of the Fab VH chain and restricted peptide, and a complete list of the interacting residues from the Fab VH chain and restricted peptide, is shown in  FIG. 39 . 
     A complete interface summary of the Fab VL chain and restricted peptide, and a complete list of the interacting residues from the Fab VL chain and restricted peptide, is shown in  FIG. 40 . 
     Fab-HLA Interactions 
     The Fab and the HLA moieties interacts extensively as shown by interface area between the HLA and the Fab with a total of 1032 Å 2 . The interaction between the HLA and the VH chain is composed of hydrophobic interactions, 6 H bonds and 3 salt bridges ( FIG. 35 , interaction between VH and HLA; and  FIG. 36 , interaction between VL and HLA). This interaction represents the major interaction are with 747 Å 2  (72% of the total contact area). 
     A table of the hydrogen bond contacts between the VH chain of the Fab and the HLA protein is shown below. 
     
       
         
           
               
             
               
                 TABLE 28 
               
             
            
               
                   
               
               
                 hydrogen bond contacts between VH and HLA. 
               
            
           
           
               
               
               
            
               
                 Fab VH 
                 Distance 
                 HLA 
               
               
                   
               
               
                 C:SER 31[ OG ] 
                 2.71 
                 A:THR 164[ OG1 ] 
               
               
                 C:TYR 100A[ OH ] 
                 2.55 
                 A:THR 164[ OG1 ] 
               
               
                 C:SER 31[ N ] 
                 3.17 
                 A:GLU 167[ OE1 ] 
               
               
                 C:SER 30[ N ] 
                 2.86 
                 A:GLU 167[ OE2 ] 
               
               
                 C:TYR 32[ OH ] 
                 2.80 
                 A:LYS 67[ NZ ] 
               
               
                 C:TYR 98[ O ] 
                 2.94 
                 A:ARG 66[ NH2 ] 
               
               
                 C:ASP 100[ OD1 ] 
                 2.88 
                 A:ARG 66[ NH1 ] 
               
               
                   
               
            
           
         
       
     
     A table of the salt bridge contacts between the VH chain of the Fab and the HLA protein is shown below. 
     
       
         
           
               
             
               
                 TABLE 29 
               
             
            
               
                   
               
               
                 salt bridge contacts between VH and HLA. 
               
            
           
           
               
               
               
            
               
                 Fab VH 
                 Distance 
                 HLA 
               
               
                   
               
               
                 C:ASP 100[ OD1 ] 
                 2.88 
                 A:ARG 66[ NH1 ] 
               
               
                 C:ASP 100[ OD1 ] 
                 3.39 
                 A:ARG 66[ NH2 ] 
               
               
                 C:ASP 100[ OD2 ] 
                 3.40 
                 A:ARG 66[ NH1 ] 
               
               
                   
               
            
           
         
       
     
     A complete interface summary of the Fab VH chain HLA protein is shown in  FIG. 41 . 
     A complete list of the interacting residues from the Fab VH chain and HLA protein is shown in  FIG. 42 . 
     A table of the hydrogen bond contacts between the VL chain of the Fab and the HLA protein is shown in Table 30 below. 
     
       
         
           
               
             
               
                 TABLE 30 
               
             
            
               
                   
               
               
                 hydrogen bonds between VL and HLA. 
               
            
           
           
               
               
               
            
               
                 Fab VL 
                 Distance 
                 HLA 
               
               
                   
               
               
                 D:ILE 94[ N ] 
                 3.56 
                 A:ALA 151[ O ] 
               
               
                 D:SER 30[ OG ] 
                 2.84 
                 A:GLN 73[ NE2 ] 
               
               
                 D:ILE 94[ O ] 
                 3.00 
                 A:HIS 152[ ND1 ] 
               
               
                   
               
            
           
         
       
     
     A complete interface summary of the Fab VL chain HLA protein is shown in  FIG. 43 . 
     A complete list of the interacting residues from the Fab VL chain and HLA protein is shown in  FIG. 44 . 
     Example 16: Identification of Predicted HLA-PEPTIDE Complexes 
     We identified cancer specific HLA-peptide targets using three computational steps: First, we identified genes that are not generally expressed in most normal tissues using data available through the Genotype-Tissue Expression (GTEx) Project [1]. We then identified which of those genes are aberrantly expressed in cancer samples using data from The Cancer Genome Atlas (TCGA) Research Network: http://cancergenome.nih.gov/. In these genes, we identified which peptides are likely to be presented as cell surface antigens by MHC Class I proteins using a deep learning model trained on HLA presented peptides sequenced by MS/MS, as described in international patent application no. PCT/US2016/067159, herein incorporated by reference, in its entirety, for all purposes. 
     To identify genes that are not usually expressed in normal tissues, we obtained aggregated gene expression data from the Genotype-Tissue Expression (GTEx) Project (version V6p). This dataset comprised 8,555 post-mortem samples from over 50 tissue types. Expression was measured using RNA-Seq and computationally processed according to the GTEx standard pipeline (https://www.gtexportal.org/home/documentationPage). For the purposes of this analysis, genes were considered not expressed in normal tissues if they were found not to be expressed in any tissues in GTEx or were only expressed in one or more of testis, minor salivary gland, and the endocervix (i.e., immune privileged or non-essential tissues). We also restricted our search to only include protein coding genes. Because GTEx and TCGA use different annotations of the human genome in their computational analyses, we excluded genes which we could not map between the two datasets using standard techniques such as ENCODE mappings. 
     We sought to define criteria to excluded genes that were expressed in normal tissue that was strict to ensure tumor specificity, but would not exclude non-zero measurements arising from sporadic, low level transcription or potential artifacts such as read misalignment. Therefore, we designated a gene to be not normally expressed in a non-immune privileged or essential tissue if its median expression across GTEx samples was less than 0.5 RPKM (Reads Per Kilobase of transcript per Million mapped reads), and it was never expressed with greater than 10 RPKM, and it was expressed at 5 RPKM in no more than two samples across all essential tissue samples. To exclude genes which were potentially expressed but could not be measured by RNA-Seq using the GTEX analysis pipeline, we also excluded genes which were measured at 0 RPKM in all samples. These criteria left us with a set of protein coding genes that did not appear to be expressed in most normal tissues. 
     We next sought to identify which of these genes are aberrantly expressed in tumors. We examined 11,093 samples available from TCGA (Data Release 6.0). We considered a gene expressed if it was observed at expression of at least 5 FPKM (Fragments Per Kilobase of transcript per Million mapped reads) in at least 5 samples. Because one fragment usually consists of two mapped reads, 5 FPKM equals approximately 10 RPKM. 
     While the GTEx data spans a broad range of tissue types, it does not include all cell types that are present in the human body. We therefore further examined the list for the gene&#39;s biological function category using the DAVID v 6.8 [2] and used this analysis, along with literature review, to filter the gene list further. We removed genes likely to be expressed in immune cells (e.g., interferon family genes), eye-related genes (e.g., retina in the FANTOM5 dataset http://www.proteinatlas.org), genes expressed in the mouth and nose (e.g. olfactory genes and taste receptors), and genes related to the circadian cycle. We also excluded genes that are part of large gene families, including histone genes, because their expression is difficult to accurately assess with RNA Sequencing due to sequence homology. 
     We then examined the distribution of the expression of the remaining genes across the TCGA samples. When we examined the known Cancer Testis Antigens (CTAs), e.g., the MAGE family of genes, we observed that the expression of these genes in log space was generally characterized by a bimodal distribution across samples in the TCGA. This distribution included a left mode around a lower expression value and a right mode (or thick tail) at a higher expression level. This expression pattern is consistent with a biological model in which some minimal expression is detected at baseline in all samples and higher expression of the gene is observed in a subset of tumors experiencing epigenetic dysregulation. We reviewed the distribution of expression of each gene across TCGA samples and discarded those where we observed only a unimodal distribution with no significant right-hand tail, as this distribution may (as a non-limiting example) more likely characterize genes that have a low baseline of expression in normal tissues. 
     This left us with a remaining gene list of &gt;630 genes that was highly enriched for genes involved in testis-specific biological processes and development. Because many of these genes produce different isoforms, these genes mapped to &gt;1,200 proteins using the UNIPROT mapping service. In addition to the genes that met our strict computational criteria, we added several genes that have previously been identified in the scientific literature as cancer testes antigens. 
     To identify the peptides that are likely to be presented as cell surface antigens by MHC Class I proteins, we used a sliding window to parse each of these proteins into its constituent 8-11 amino acid sequences. We processed these peptides and their flanking sequences with the HLA peptide presentation deep learning model to calculate the likelihood of presentation of each peptide at expression levels between five TPM, which approximately corresponds to one transcript per cell [3], to 200 TPM (i.e., a high level of expression). We considered a peptide a putative HLA-PEPTIDE target if its probability of presentation calculated by our model was greater than 0.1 in 10 or more patients in the TCGA dataset with expression 5 TPM or greater. 
     The results are shown in Table A1. From this example, there are &gt;1,800 HLA-PEPTIDE targets across ˜400 genes and 25 analyzed HLA alleles. For clarity, each HLA-PEPTIDE was assigned a target number in Table A1. For example, HLA-PEPTIDE target 1 is HLA-A*01:01_EVDPIGHLY, HLA-PEPTIDE target 2 is HLA-A*29:02_FVQENYLEY, and so forth. 
     Collectively, this list of HLA-PEPTIDE targets is expected to be a significant contribution to the state of knowledge of cancer specific targets. In summary, the example provides a large set of tumor-specific HLA-PEPTIDEs that can be pursued as candidate targets for ABP research and development. 
     REFERENCES 
     
         
         1. Consortium, G. T.,  The Genotype - Tissue Expression  ( GTEx ) project . Nat Genet, 2013. 45(6): p. 580-5. 
         2. Huang da, W., B. T. Sherman, and R. A. Lempicki, Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc, 2009. 4(1): p. 44-57. 
         3. Shapiro, E., T. Biezuner, and S. Linnarsson,  Single - cell sequencing - based technologies will revolutionize whole - organism science . Nat Rev Genet, 2013. 14(9): p. 618-30. 
       
    
     Example 17: Initial Validation of Predicted HLA-PEPTIDE Complexes 
     As an initial assessment to validate the predicted HLA-PEPTIDE targets arising from the above described approach, we evaluated public databases and selected literature for reports of these targets as having been previously identified by various assay techniques, including HLA binding affinity measurements, HLA peptide mass-spectrometry, as well as measures of T cell responses. Two comprehensive databases containing assay result annotations for HLA-PEPTIDE pairs were used: IEDB (Vita et al., 2015) and Tantigen (Olsen et al., 2017). We determined that 19 (15 unique across genes) of the computationally predicted targets were previously reported in the databases, many in genes (e.g., cancer testis antigens) that have long been the subject of study in cancer immunology. See Table B. 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE B 
               
               
                   
               
               
                   
                   
                 Found in 
                   
                   
               
               
                 Protein 
                   
                 IEDB or 
                 IEDB 
                 Tantigen 
               
               
                 Name 
                 HLA-PEPTIDE 
                 Tantigen 
                 Status 
                 Status 
               
               
                   
               
             
            
               
                 MAGA3 
                 HLA-A*01:01_EVDPIGHLY 
                 TRUE 
                 Found 
                 Found 
               
               
                   
               
               
                 MAGA3 
                 HLA-A*29:02_FVQENYLEY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGA3 
                 HLA-A*29:02_LVHFLLLKY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGA3 
                 HLA-B*44:03_MEVDPIGHLY 
                 TRUE 
                 Not found 
                 Found 
               
               
                   
               
               
                 MAGA6 
                 HLA-A*29:02_FVQENYLEY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGA6 
                 HLA-A*29:02_LVHFLLLKY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGA4 
                 HLA-A*01:01_EVDPASNTY 
                 TRUE 
                 Not found 
                 Found 
               
               
                   
               
               
                 MAGA1 
                 HLA-A*02:01_KVLEYVIKV 
                 TRUE 
                 Found 
                 Found 
               
               
                   
               
               
                 MAGAC 
                 HLA-A*29:02_LVHFLLLKY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGAC 
                 HLA-A*29:02_LVQENYLEY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 SSX1 
                 HLA-C*04:01_AFDDIATYF 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGA4 
                 HLA-A*29:02_WVQENYLEY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGB2 
                 HLA-A*02:01_GVYDGEEHSV 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGA1 
                 HLA-A*03:01_SLFRAVITK 
                 TRUE 
                 Found 
                 Found 
               
               
                   
               
               
                 MAGA4 
                 HLA-A*11:01_ALAETSYVK 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 SAGE1 
                 HLA-A*24:02_LYATVIHDI 
                 TRUE 
                 Not found 
                 Found 
               
               
                   
               
               
                 PASD1 
                 HLA-A*02:01_QLLDGFMITL 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGA8 
                 HLA-A*29:02_WVQENYLEY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
               
                 MAGAC 
                 HLA-A*29:02_STLPTTINY 
                 TRUE 
                 Found 
                 Not found 
               
               
                   
               
            
           
         
       
     
     Additional limited literature review was carried out for peptides not found in the above public databases. The following peptides were identified, as shown in Table C: 
     
       
         
           
               
               
               
               
             
               
                 TABLE C 
               
               
                   
               
               
                   
                   
                 HLA/peptide known 
                 HLA/peptide known status in 
               
               
                 HLA 
                 Protein 
                 status IEDB or 
                 literature (preliminary) if 
               
               
                 allele/peptide complex 
                 Name 
                 Tantig 2017 
                 not in IEDB or Tantigen 
               
               
                   
               
             
            
               
                 HLA-A*01:01_NTDNNLAVY 
                 KKLC1 
                 Not known 
                 WO 2017/089756 A1 
               
               
                   
                   
                   
                 (Stevanović etal., 2017) 
               
               
                   
               
               
                 HLA-B*35:01_YPAPLESLDY 
                 PRA10 
                 Not known 
                 WO2008118017 A2 
               
               
                   
               
               
                 HLA-A*11:01_ATLENLLSH 
                 PRAM4 
                 Not known 
                 WO2008118017 A2 
               
               
                   
               
               
                 HLA-B*51:01_DALLAQKV 
                 PRA12 
                 Not known 
                 WO2008118017 A2 
               
               
                   
               
               
                 HLA-B*44:03_SESDLKHLSW 
                 PRA12 
                 Not known 
                 WO2008118017 A2 
               
               
                   
               
               
                 HLA-A*11:01_ATLENLLSH 
                 PRAM9 
                 Not known 
                 WO2008118017 A2 
               
               
                   
               
               
                 HLA-A*02:07_TLDEYLTYL 
                 PRAM9 
                 Not known 
                 WO2008118017 A2 
               
               
                   
               
            
           
         
       
     
     One notable example from Table C was KKLC1 HLA-A*01:01_NTDNNLAVY Kita-kyushu lung cancer antigen-1 (KK-LC-1; CT83) is a cancer testis antigen (CTA) that has been shown to be widely expressed in many different cancer types. It was originally discovered based on a cloned CTL to KK-LC-1 peptide 76-84—RQKRILVNL (Fukuyama et al., 2006). More recently Stevanovid et al., 2017 revealed another peptide from KK-LC-1 recognized by a CTL in a patient with cervical cancer, the predicted peptide KK-LC-1 52-60 NTDNNLAVY The corresponding TCR for this CTL is now listed on the NIH website https://www.ott.nih.gov/technology/e-153-2016/and the peptide is listed in WO 2017/089756 A1, herein incorporated by reference, in its entirety, for all purposes. 
     This example highlights the expected value of predicted HLA-PEPTIDE targets in Table A: Although no information on which CTA HLA-PEPTIDE targets were previously known was incorporated in the prediction, the analysis yielded many targets that were described in the literature, indicating that many of the novel targets can likewise be validated experimentally and ultimately serve as targets for one or more ABPs. 
     REFERENCES 
     
         
         Fukuyama, T., Hanagiri, T., Takenoyama, M., Ichiki, Y, Mizukami, M., So, T., Sugaya, M., So, T., Sugio, K., and Yasumoto, K. (2006). Identification of a new cancer/germline gene, KK-LC-1, encoding an antigen recognized by autologous CTL induced on human lung adenocarcinoma. Cancer Res. 66, 4922-4928. 
         Olsen, L. R., Tongchusak, S., Lin, H., Reinherz, E. L., Brusic, V., and Zhang, G. L. (2017). TANTIGEN: a comprehensive database of tumor T cell antigens. Cancer Immunol. Immunother. CII 66, 731-735. 
         Stevanović, S., Pasetto, A., Helman, S. R., Gartner, J. J., Prickett, T. D., Howie, B., Robins, H. S., Robbins, P. F., Klebanoff, C. A., Rosenberg, S. A., et al. (2017). Landscape of immunogenic tumor antigens in successful immunotherapy of virally induced epithelial cancer. Science 356, 200-205. 
         Vita, R., Overton, J. A., Greenbaum, J. A., Ponomarenko, J., Clark, J. D., Cantrell, J. R., Wheeler, D. K., Gabbard, J. L., Hix, D., Sette, A., et al. (2015). The immune epitope database (IEDB) 3.0. Nucleic Acids Res. 43, D405-412. 
       
    
     Example 18: Identification of Predicted HLA-PEPTIDE Complexes 
     Next, HLA-peptide targets from proteins of seven genes were identified: AFP, KKLC-1, MAGE-A4, MAGE-A10, MART-1, NY-ESO-1, and WT1. 
     To identify peptides that are likely to be presented as cell surface antigens by MHC Class I proteins, a sliding window was used to parse each of these proteins into its constituent 8-11 amino acid sequences. These peptides and their flanking sequences were then processed with the HLA peptide presentation deep learning model (see PCT/US2016/067159 and Example 16 above) to calculate the likelihood of presentation of each peptide at an expression level of 100 TPM (high expression) for each of 64 Class I HLA types. Potential modeling artifacts were removed that could give stronger scores to certain HLAs due to training data biases by quantile normalizing model scores for each HLA so that each HLA present scores from the same distribution. In the normalization, the seven target genes as well as 50 randomly selected genes were included to control for HLA allele sequence preferences. A gene was considered likely to be presented if the model normalized score was higher than 0.00075, which was chosen based on the presentation scores of peptides known to be presented in the literature. 
     The results are shown in Table A2. Target numbers were assigned to each HLA-PEPTIDE target as described in Example 16. 
     Example 19: Identification of Antibodies or Antigen-Binding Fragments Thereof that Bind HLA-PEPTIDE Complexes 
     Overview 
     The following exemplification demonstrates that antibodies (Abs) can be identified that recognize tumor-specific HLA-restricted peptides. The overall epitope that is recognized by such Abs generally comprises a composite surface of both the peptide as well as the HLA protein presenting that particular peptide. Abs that recognize HLA complexes in a peptide-specific manner are often referred to as T cell receptor (TCR)-like Abs or TCR-mimetic Abs. The HLA-PEPTIDE target antigens that were selected for antibody discovery are HLA-A*01:01_NTDNNLAVY (Target 33 in Table A1 designated as “G2”) and HLA-A*02:01_LLASSILCA (Target 6427 in Table A2, designated as “G7”). Cell surface presentation of these HLA-PEPTIDE antigens was confirmed by mass spectrometry analysis of HLA complexes obtained from tumor samples, as described in Example 2. 
     Generation of HLA-PEPTIDE Target Complexes and Counterscreen Peptide-HLA Complexes, and Stability Analysis 
     The HLA-PEPTIDE targets G2 and G7, as well as counterscreen negative control peptide-HLAs, were produced recombinantly using conditional ligands for HLA molecules using established methods. In all, 18 counterscreen HLA-peptides were generated for each of the G2 and G7 targets. 
     Overall Design of Phage Library Screening 
     The highly diverse SuperHuman 2.0 synthetic naïve scFv library from Distributed Bio Inc (7.6e10 total diversity on ultra-stable and diverse VH/VL scaffolds) was used for phage display. The phage library was initially depleted with 18 pooled negative pHLA complexes (the “complete pool”) followed by three to four rounds of bead-based phage panning with the target pHLA complex using established protocols to identify scFv binders to HLA-PEPTIDE targets G2 and G7, respectively. The phage titer was determined at every round of panning to establish removal of non-binding phage. Phage ELISA results are shown in  FIGS. 70A and 70B . There was an enrichment of bound phage in later rounds of panning for each of the G2 and G7 targets. The output phage supernatant was also tested for target binding by ELISA. 
     The design of target screen 1 for the G2 target is shown in  FIG. 64 . Similarly, the design of target screen 2 for the G7 target is shown in  FIG. 67 . Briefly, for each target, three “minipool” counterscreen peptides were selected for their ability to bind the same HLA allele as the target and also to have significantly different ABP-facing features such as charge, bulk, aromatic, or hydrophobic residues. See  FIG. 65A  for G2 and  FIG. 69A  for G7. In addition, additional counterscreen peptide-HLA complexes, featuring distinct restricted peptide sequences and different HLA alleles were generated. The 15 additional counterscreen HLA-peptides plus the three “minipool” HLA-peptides formed a “complete pool” of 18 total counterscreen HLA-peptide complexes. 
     Generation of Peptide-HLA Complexes 
     α-, and β2 microglobulin chain of various human leukocyte antigens (HLA) were expressed separately in BL21 competent  E. Coli  cells (New England Biolabs) using established procedures (Garboczi, Hung, &amp; Wiley, 1992). Following auto-induction, cells were lysed via sonication in Bugbuster® plus benzonase protein extraction reagent (Novagen). The resulting inclusion bodies were washed and sonicated in wash buffer with and without 0.5% Triton X-100 (50 mM Tris, 100 mM NaCl, 1 mM EDTA). After the final centrifugation, inclusion pellets were dissolved in urea solution (8 M urea, 25 mM MES, 10 mM EDTA, 0.1 mM DTT, pH 6.0). Bradford assay (Biorad) was used to quantify the concentration and the inclusion bodies were stored at −80° C. 
     HLA complexes were obtained by refolding of recombinantly produced subunits and a synthetically obtained peptide using established procedures. (Garboczi et al., 1992). Briefly, the purified α and β2 microglobulin chains were refolded in refold buffer (100 mM Tris pH 8.0, 400 mM L-Arginine HCl, 2 mM EDTA, 50 mM oxidized glutathione, 5 mM reduced glutathione, protease inhibitor tablet) with the restricted peptide of choice. In some experiments, the restricted peptide of choice was a conditional ligand peptide, which is cleavable upon exposure to a conditional stimulus. In some experiments, the restricted peptide of choice was the G2 or G7 target peptide, or counterscreen peptide. The refold solution was concentrated with a Vivaflow 50 or 50R crossflow cassette (Sartorius Stedim). Three rounds of dialyses in 20 mM Tris pH 8.0 were performed for at least 8 hours each. For the antibody screening and functional assays, the refolded HLA was enzymatically biotinylated using BirA biotin ligase (Avidity). Refolded protein complexes were purified using a HiPrep (16/60 Sephacryl S200) size exclusion column attached to an Akta FPLC system. Biotinylation was confirmed in a streptavidin gel-shift assay under non-reducing conditions by incubating the refolded protein with an excess of streptavidin at room temperature for 15 minutes prior to SDS-PAGE. The resulting peptide-HLA complexes were aliquoted and stored at −80° C. 
     Stability Analysis of the Peptide-HLA Complexes 
     HLA-peptide stability was assessed by conditional ligand peptide exchange and stability ELISA assay. Briefly, conditional ligand-HLA complexes were subjected to ±conditional stimulus in the presence or absence of the counterscreen or test peptides. Exposure to the conditional stimulus cleaves the conditional ligand from the HLA complex, resulting in dissociation of the HLA complex. If the counterscreen or test peptide stably binds the α1/α2 groove of the HLA complex, it “rescues” the HLA complex from disassociation. 
     The HLA stability ELISA was performed using established procedures. (Chew et al., 2011; Rodenko et al., 2006) A 384-well clear flat bottom polystyrene microplate (Corning) was precoated with 50 μl of streptavidin (Invitrogen) at 2 μg mL −1  in PBS. Following 2 h of incubation at 37° C., the wells were washed with 0.05% Tween 20 in PBS (four times, 50 μL) wash buffer, treated with 50 μl of blocking buffer (2% BSA in PBS), and incubated 30 min at room temperature. Subsequently, 25 μl of peptide-exchanged samples that were 300× diluted with 20 mM Tris HCl/50 mM NaCl were added in quadruplicate. The samples were incubated for 15 min at RT, washed with 0.05% Tween wash buffer (4×50 L), treated for 15 min with 25 μL of HRP-conjugated anti-02m (1 μg mL −1  in PBS) at RT, washed with 0.05% Tween wash buffer (4×50 μL), and developed for 10-15 min with 25 L of ABTS-solution (Invitrogen), and the reactions were stopped by the addition of 12.5 μL of stop buffer (0.01% sodium azide in 0.1 M citric acid). Absorbance was subsequently measured at 415 nm using a spectrophotometer (SpectraMax i3x; Molecular Devices). 
     Results for the G2 counterscreen “minipool” and G2 target are shown in  FIG. 65B . All three counterscreen peptides and the G2 peptide rescued the HLA complex from dissociation. 
     Results for the additional G2 “complete” pool counterscreen peptides are shown in  FIG. 66 , demonstrating that they also form stable HLA-peptide complexes. 
     Results for the G7 counterscreen “minipool” and G7 target are shown in  FIG. 69B . All three counterscreen peptides and the G7 peptide rescued the HLA complex from dissociation. 
     Results for the additional G7 “complete” pool counterscreen peptides are shown in  FIG. 68 , demonstrating that they also form stable HLA-peptide complexes. 
     Phage Library Screening 
     Phage library screening was carried out according to the overall screening design described above. Three to four rounds of bead-based panning were performed to identify scFv binders to each peptide-HLA complex. For each round of panning, an aliquot of starting phage was set aside for input titering and the remaining phage was depleted three times against Dynabead M-280 streptavidin beads (Life Technologies) followed by a depletion against Streptavidin beads pre-bound with 100 pmoles of pooled negative peptide-HLA complexes. For the first round of panning, 100 pmoles of peptide-HLA complex bound to streptavidin beads was incubated with depleted phage for 2 hours at room temperature with rotation. Three five-minute washes with 0.5% BSA in 1×PBST (PBS+0.05% Tween-20) followed by three five-minute washes with 0.5% BSA in 1×PBS were utilized to remove any unbound phage to the peptide-HLA complex bound beads. To elute the bound phage from the washed beads, 1 ml 0.1M TEA was added and incubated for 10 minutes at room temperature with rotation. The eluted phage was collected from the beads and neutralized with 0.5 ml 1M Tris-HCl pH 7.5. The neutralized phage was then used to infect log growth TG-1 cells (OD 600 =0.5) and after an hour of infection at 37° C., cells were plated onto 2YT media with 100 μg/ml carbenicillin and 2% glucose (2YTCG) agar plates for output titer and bacterial growth for subsequent panning rounds. For subsequent rounds of panning, selection antigen concentrations were lowered while washes increased by amount and length of wash times at show in Table 31. 
     
       
         
           
               
             
               
                 TABLE 31 
               
             
            
               
                   
               
               
                 Phage library screening strategy 
               
            
           
           
               
               
               
            
               
                 Round 
                 Antigen concentration 
                 Washes 
               
               
                   
               
               
                 R1 
                 100 pmol 
                 3X PBST + 3X PBS (5 min washes) 
               
               
                 R2 
                  25 pmol 
                 5 PBST (2x 30 sec, 3x 5 min) +  
               
               
                   
                   
                 5 PBS (2x 30 sec, 3x 5 min) 
               
               
                 R3 
                  10 pmol 
                 8 PBST (4x 30 sec, 4x 5 min) + 8 PBS  
               
               
                   
                   
                 (4x 30 sec, 4x 5 min) 
               
               
                 R4 
                 5 pmol, 10 pmol 
                 30 min PBST + 30 min PBS 
               
               
                   
               
            
           
         
       
     
     Individual scFvs were cloned from phage and sequenced by DNA Sanger sequencing (“Sequence Unique Binders”). The individual scFvs were also expressed in  E. coli  and periplasmic extracts (PPE) from  E. coli  containing the individual crude scFvs were subjected to scFv ELISA 
     scFv Periplasmic Extract (PPE) ELISA 
     The individual scFv cloned from phage obtained in the final round of panning, and expressed in  E. coli , was subjected to scFv PPE ELISA as follows. 
     96-well and/or 384-well streptavidin coated plates (Pierce) were coated with 2 ug/ml peptide-HLA complex in HLA buffer and incubated overnight at 4° C. Plates were washed three times between each step with PBST (PBS+0.05%). The antigen coated plates were blocked with 3% BSA in PBS (blocking buffer) for 1 hour at room temperature. After washing, scFv PPEs were added to the plates and incubated at room temperature for 1 hour. Following washing, mouse anti-v5 antibody (Invitrogen) in blocking buffer was added to detect scFv and incubated at room temperature for 1 hour. After washing, HRP-goat anti-mouse antibody (Jackson ImmunoResearch) was added and incubated at room temperature for 1 hour. The plates were then washed three times with PBST and 3 times with PBS before HRP activity was detected with TMB 1-component Microwell Peroxidase Substrate (Seracare) and neutralized with 2N sulfuric acid. 
     For negative peptide-HLA complex counter-screening, scFv PPE ELISAs were performed as described above, except for the coating antigen. HLA mini-pools consisted of 2 ug/ml of each of the three negative peptide-HLA complexes pooled together and coated onto streptavidin plates for comparison binding to their particular peptide-HLA complex. HLA big pools consisted of 2 ug/ml of each of all 18 negative peptide-HLA complexes pooled together and coated onto streptavidin plates for comparison binding to their particular peptide-HLA complex. 
     Those scFvs that showed selectivity for target pHLA compared to negative control pHLA by scFv-ELISA as crude PPE, were separately expressed and purified. The purified scFvs were titrated by scFv ELISA for confirmation of binding only target pHLA compared to negative control pHLA (“Selective Binders”). 
     Clones were formatted into IgG, Fab, or scFv for further biochemical and functional analysis. ScFv clones selected for Fab production to be used for crystallization with their corresponding pHLA complexes were selected based on several parameters: sequence diversity, binding affinity, selectivity, and CDR3 diversity. The clustal software was used to produce a dendrogram and assess the sequence diversity of the Fab clones. The canonical 3D structures of the scFv sequences, based on the VH type, were also considered when possible. Binding affinity, as determined by the equilibrium dissociation constant (KD), was measured using an Octet HTX (ForteBio). Selectivity for the specific peptide-HLA complexes was determined with an ELISA titration of the purified scFvs and compared to negative peptides or streptavidin alone. Cutoff values for the KD and selectivity were determined for each target set based on the range of values obtained for the Fabs within each set. Final clones were then selected to obtain the highest diversity in sequence families and CDR3. 
     Table 32 shows the hit rate for the screening campaign described above. 
     
       
         
           
               
             
               
                 TABLE 32 
               
             
            
               
                   
               
               
                 hit rate for screening campaigns 
               
            
           
           
               
               
               
            
               
                 Group 
                 G2 
                 G7 
               
               
                   
               
               
                 Gene target 
                 CT83 
                 CT83 
               
               
                 HLA 
                 A*01:01 
                 A*02:01 
               
               
                 Restricted peptide 
                 NTDNNLAVY 
                 LLASSILCA 
               
               
                 # Sequence Unique 
                 74 
                 8 
               
               
                 Binders 
                   
                   
               
               
                 # Selective Binders 
                 27 
                 6 
               
               
                 # selected for IgG 
                 20 
                 8 
               
               
                 # selected for Fab 
                 6 
                 3 
               
               
                 # selected for scFv 
                 20 
                 7 
               
               
                   
               
            
           
         
       
     
     Table 33 shows the VH and VL sequences of the G2 scFv Selective Binders, selective for HLA-PEPTIDE Target HLA-A*01:01_NTDNNLAVY 
     Table 34 shows the CDR sequences for the G2 Selective Binders, selective for HLA-PEPTIDE Target HLA-A*01:01_NTDNNLAVY CDRs were determined according to the Kabat numbering system._ 
     Table 35 shows the VH and VL sequences of the G7 scFv Selective Binders, selective for HLA-PEPTIDE Target HLA-A*02:01_LLASSILCA. 
     Table 36 shows the CDR sequences for the G7 Selective Binders, selective for HLA-PEPTIDE Target HLA-A*02:01_LLASSILCA. CDRs were determined according to the Kabat numbering system. 
     Example 20: Isolation of TCRs that Specifically Bind HLA-PEPTIDE Targets 
       FIG. 81  depicts an experimental workflow by which TCRs which specifically bind HLA-PEPTIDE targets were isolated. Briefly, naïve CD8+ T cells that bind to the HLA-PEPTIDE target were isolated by flow cytometry and polyclonally expanded. Following expansion, specificity of cells for HLA-PEPTIDE target complex was tested by flow cytometry. If a large fraction (&gt;75%) of an expanded population was specific for the HLA-PEPTIDE target, the population as a whole was sequenced as a whole to identify TCRs. Alternatively, cells that specifically bound the HLA-PEPTIDE target were resorted, and only cells isolated after resort were sequenced. TCR sequences were cloned into expression vectors and introduced into recipient T cells as recombinant TCRs. Expression of the evaluated TCR and binding of cognate HLA-PEPTIDE target complex by the TCR-recombinant T cells was assessed. 
     Identified HLA-PEPTIDE Targets were Readily Recognized by CD8+ T Cells 
     Peripheral Blood Mononuclear Cells (PBMCs) from healthy donors were magnetically enriched for naïve CD8+ T cells as follows. PBMCs were obtained by processing leukapheresis samples from healthy donors. Frozen PBMCs were thawed and incubated with cocktail of biotinylated CD45RO, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD57, CD123, anti-HLA-DR, CD235a (Glycophorin A), CD244, and CD4 antibodies and were subsequently magnetically labeled with anti-biotin microbeads for removal from PBMC population. Enriched naïve CD8 T cells were labelled with tetramers comprising of target peptide and appropriate HLA molecule, stained with live/dead and lineage markers and sorted by flow cytometry according to the gating procedure depicted in  FIG. 82 . Cells that bound the HLA-PEPTIDE tetramers were isolated. Following polyclonal expansion, specificity of expanded CD8+ T cells was reassessed by labeling with the HLA-PEPTIDE or no tetramer control. Flow cytometry results for exemplary HLA-PEPTIDE targets B*44:02_GEMSSNSTAL and A*01:01_EVDPIGHLY are shown in  FIG. 83 . Flow cytometry results for the HLA-PETPIDE target A*03:01_GVHGGILNK is shown in  FIG. 84 . 
     The number of isolated CD8+ T cells per HLA-PEPTIDE target per donor and distribution of isolated CD8+ T cells frequency per HLA-PEPTIDE target across all donors tested is shown in  FIGS. 85A  (number of isolated CD8+ T cells) and  85 B (frequency). Total number of isolated naïve CD8+ T cells per target ranged from 23-4181 antigen specific cells, which is in line with precursor frequencies of T cells specific for known immunogenic viral antigens. These cells present the source of natural TCRs for sequencing and further characterization. 
     The number of isolated target-specific T cells per target summarized across all tested donors is shown in Table 37 
     
       
         
           
               
             
               
                 TABLE 37 
               
             
            
               
                   
               
               
                 number of isolated target-specific T cells per 
               
               
                 target summarized across all donors 
               
            
           
           
               
               
               
            
               
                   
                   
                 Cumulative Number of TCR 
               
               
                 Target 
                 Gene 
                 Source Cells Per Target 
               
               
                   
               
               
                 EVDPIGHLY 
                 MAGEA3 
                 5242 
               
               
                 (HLA-A*0101) 
                   
                   
               
               
                   
               
               
                 EVDPIGHVY 
                 MAGEA6 
                 1296 
               
               
                 (HLA-A*0101 
                   
                   
               
               
                   
               
               
                 GEMS SNSTAL 
                 CT83 
                   48 
               
               
                 (HLA-B*4402) 
                   
                   
               
               
                   
               
               
                 GVHGGILNK 
                 PFN3 
                  219 
               
               
                 (HLA-A*0301) 
                   
                   
               
               
                   
               
               
                 GVYDGEEHSV 
                 MAGEB2 
                   17 
               
               
                 (HLA-A*0201) 
                   
                   
               
               
                   
               
               
                 LLASSILCA 
                 CT83 
                 1665 
               
               
                 (HLA-A*0201) 
                   
                   
               
               
                   
               
               
                 LVIDTVTEV 
                 SPERT 
                   16 
               
               
                 (HLA-A*0201) 
                   
                   
               
               
                   
               
               
                 NTDNNLAVY 
                 CT83 
                  575 
               
               
                 (HLA-A*0101) 
               
               
                   
               
            
           
         
       
     
     These data demonstrate that identified HLA-PEPTIDE targets are biologically relevant, as natural CD8+ T cells exist in HLA matched human blood which bind/recognize target peptides in the context of predicted associated MHC molecule. 
     CD8+ T Cells Yielded a Diverse Repertoire of Unique TCRs which Bound the HLA-PEPTIDE Targets 
     Criteria for Sequencing of T-Cells 
     If a large fraction (&gt;75%) of an expanded population was specific for the HLA-PEPTIDE target, the population as a whole was sequenced as a whole to identify TCRs. Then, selected TCR sequences from the population were cloned into expression vectors and transfected into recipient T-cells for confirmation of specificity. Alternatively, cells that specifically bound the HLA-PEPTIDE target were resorted, and only cells isolated after resort were sequenced. 
     Sequencing Protocol 
     T cells isolated and expanded as described in  FIG. 82  were sequenced using 10× Genomics single cell resolution paired immune TCR profiling approach. Specifically, two-to-eight thousand live T cells were partitioned into single cell emulsions for subsequent single cell cDNA generation and full-length TCR profiling (5′ UTR through constant region—ensuring alpha and beta pairing). One approach utilizes a molecularly barcoded template switching oligo at the 5′ end of the transcript, a second approach utilizes a molecularly barcoded constant region oligo at the 3′ end, and a third approach couples an RNA polymerase promoter to either the 5′ or 3′ end of a TCR. All of these approaches enable the identification and deconvolution of alpha and beta TCR pairs at the single-cell level. The resulting barcoded cDNA transcripts underwent an optimized enzymatic and library construction workflow to reduce bias and ensure accurate representation of clonotypes within the pool of cells. Libraries were sequenced on Illumina&#39;s MiSeq or HiSeq4000 instruments (paired-end 150 cycles) for a target sequencing depth of about five to fifty thousand reads per cell. 
     Sequencing reads were processed through the 10× provided software Cell Ranger. Sequencing reads were tagged with a Chromium cellular barcodes and UMIs, which were used to assemble the V(D)J transcripts cell by cell. The assembled contigs for each cell were then annotated by mapping the assembled contigs to V(D)J reference sequences from Ensembl version 87 (http://www.ensembl.org/). 
     Clonotypes were defined as alpha, beta chain pairs of unique CDR3 amino acid sequences. Clonotypes were filtered for single alpha and single beta chain pairs present at frequency above 2 cells to yield the final list of clonotypes per target peptide in a specific donor.  FIG. 86A  depicts the number of unique TCR clonotypes per HLA-PEPTIDE target for each tested donor.  FIG. 86B  depicts the total number of unique clonotypes per HLA-PEPTIDE target, summed across all donors tested. 
     TCR Sequences of Unique Clonotypes from Resorted Cells 
     Annotated variable, diversity, joining, and constant regions of TCR clonotypes specific for A*0101_EVDPHIGHLY, from resorted cells, are shown in Table 9 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     V(D)J and CDR3 sequences of α and β chains of the TCR clonotypes specific for A*0101_EVDPHIGHLY are shown in Table 10 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     Annotated variable, diversity, joining, and constant regions of TCR clonotypes that demonstrated confirmed specificity in recipient T-cells is shown in Table 11 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     V(D)J and CDR3 sequences of α and β chains of TCR clonotypes that demonstrated confirmed specificity in recipient T-cells is shown in Table 12 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     A table of the annotated reference α variable (TRAV), α joining (TRAJ), α constant (TRAC), β variable (TRBV), β diversity (TRBD), β joining (TRBJ), and β constant (TRBC) sequences and their corresponding Ensembl transcript (ENST) reference number is shown in Table 13 of PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. For any of the TCRs disclosed, amino acid sequences that are at least 95%, at least 96%, at least 97%, and least 98%, at least 99%, or more than 99% identical to the annotated reference sequences as disclosed herein. 
     Example 21: T Cell Line Transiently Transfected with Identified TCRs Specifically Bind to their Target HLA-PEPTIDE Complex, but not to Negative Control Peptide-HLAs 
     Jurkat TIB-152 T cell line cultures were co-transfected with a plasmid expressing human CD8 and a plasmid expressing TCR α and β chains with a GFP reporter gene using Nucleofector 4D electroporator. Plasmids used for transfection are described in  FIGS. 49 and 50 . 24-48 hours post transfection, Jurkat T cells were analyzed for expression of the TCR of interest. Cells were assessed for binding to HLA-PEPTIDE complexes and a control infectious-disease-based peptide tetramer using flow cytometry. Total population was gated on live single GFP-expressing cells before evaluating binding of HLA-PEPTIDE target tetramer.  FIG. 87  shows examples of Jurkat cells expressing A*0201_LLASSILCA-, A*0201_GVYDGEEHSV-, B*4402_GEMSSNSTAL-, and A*0101_EVDPIGHLY-specific TCRs binding to their respective HLA-PEPTIDE targets but not to the control peptide tetramer. 
     Example 22: TCRs Cloned into a Viral Vector are Stably Expressed in Primary Human CD8+ T Cells and Bind Cognate Peptide Target-MHC Complexes 
     Lentiviral vectors were generated for TCR specific for the HLA-PEPTIDE target HLA-A*0201_LLASSILCA. As a model vector system, we used commercially available 3 rd  generation lentivirus base expression vector system from System Biosciences, Palo Alto, Calif. See  FIG. 89 . 
     Primary human CD8+ T cells were isolated and transduced with the recombinant TCR lentivirus at multiplicity of infection (MOI˜10). T cells were expanded using rapid expansion protocol for 1-2 weeks before assessment of TCR expression on CD8 T cells by tetramer staining. 
       FIG. 88  depicts the gating strategy and flow data demonstrating that transduced human CD8+ cells bind to the HLA-PEPTIDE target. 
     Example 23: In Vivo Proof-of-Concept 
     Lead antibody or CAR-T constructs are evaluated in vivo to demonstrate directed tumor killing in humanized mouse tumor models. Lead antibody or CAR-T constructs are evaluated in xenograft tumor models engrafted with human tumors and PBMCs. Anti-tumor activity is measured and compared to control constructs to demonstrate target-specific tumor killing. 
     While the invention has been particularly shown and described with reference to a preferred embodiment and various alternate embodiments, it will be understood by persons skilled in the relevant art that various changes in form and details can be made therein without departing from the spirit and scope of the invention. 
     All references, issued patents and patent applications cited within the body of the instant specification are hereby incorporated by reference in their entirety, for all purposes. 
     Sequences 
       
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 VH and VL sequences of scFv hits that bind target G5 
               
            
           
           
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
               
               
                 group 
                 name 
                 V H   
                 V L   
               
               
                   
               
               
                 G5 
                 G5_P7_ 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIVMTQSPLSLPVTPGEPASISCRSS 
               
               
                   
                 E7 
                 ASGYTFTSYDINWVRQAPGQGLE 
                 QSLLHSNGYNYLDWYLQKPGQSP 
               
               
                   
                   
                 WMGIINPRSGSTKYAQKFQGRVT 
                 QLLIYLGSYRASGVPDRFSGSGSGT 
               
               
                   
                   
                 MTRDTSTSTVYMELSSLRSEDTAV 
                 DFTLKISRVEAEDVGVYYCMQGL 
               
               
                   
                   
                 YYCARDGVRYYGMDVWGQGTTV 
                 QTPITFGQGTRLEIK 
               
               
                   
                   
                 TVSS 
                   
               
               
                   
               
               
                 G5 
                 G5_P7_ 
                 QVQLVQSGAEVKKPGSSVKVSCK 
                 DIVMTQSPLSLPVTPGEPASISCRSS 
               
               
                   
                 B3 
                 ASGYTFTSHDINWVRQAPGQGLE 
                 QSLLHSNGYNYLDWYLQKPGQSP 
               
               
                   
                   
                 WMGWMNPNSGDTGYAQKFQGR 
                 QLLIYLGSSRASGVPDRFSGSGSGT 
               
               
                   
                   
                 VTITADESTSTAYMELSSLRSEDTA 
                 DFTLKISRVEAEDVGVYYCMQAL 
               
               
                   
                   
                 VYYCARGVRGYDRSAGYWGQGT 
                 QTPPTFGPGTKVDIK 
               
               
                   
                   
                 LVIVSS 
                   
               
               
                   
               
               
                 G5 
                 G5_P7_ 
                 EVQLLESGGGLVKPGGSLRLSCAA 
                 DIQMTQSPSSLSASVGDRVTITCQA 
               
               
                   
                 A5 
                 SGFSFSSYWMSWVRQAPGKGLEW 
                 SQDISNYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 ISYISGDSGYTNYADSVKGRFTISR 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 DDSKNTLYLQMNSLKTEDTAVYY 
                 ISSLQPEDFATYYCQQAISFPLTFG 
               
               
                   
                   
                 CASHDYGDYGEYFQHWGQGTLV 
                 QSTKVEIK 
               
               
                   
                   
                 TVSS 
                   
               
               
                   
               
               
                 G5 
                 G5_P7_ 
                 EVQLLQSGGGLVQPGGSLRLSCAA 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 F6 
                 SGFTFSNSDMNWVRQAPGKGLEW 
                 SQSISSWLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 VAYISSGSSTIYYADSVKGRFTISR 
                 SASTLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 DNSKNTLYLQMNSLRAEDTAVYY 
                 ISSLQPEDFATYYCQQANSFPLTFG 
               
               
                   
                   
                 CARVSWYCSSTSCGVNWFDPWGQ 
                 GGTKVEIK 
               
               
                   
                   
                 GTLVTVSS 
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 EVQLLESGGGLVQPGGSLRLSCAA 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P1B12 
                 SGFTFSNSDMNWVRQAPGKGLEW 
                 SQSISSWLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 VASISSSGGYINYADSVKGRFTISR 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 DNSKNTLYLQMNSLRAEDTAVYY 
                 ISSLQPEDFATYYCQQANSFPLTFG 
               
               
                   
                   
                 CAKVNWNDGPYFDYWGQGTLVT 
                 GGTKVEIK 
               
               
                   
                   
                 VSS 
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 QVQLVQSGAEVKKPGSSVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P1C12 
                 ASGGTFSNFGVSWLRQAPGQGLE 
                 SQSISSWLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGGIIPILGTANYAQKFQGRVTI 
                 AASTLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TADESTSTAYMELSSLRSEDTAVY 
                 ISSLQPEDFATYYCQQSYSIPLTFG 
               
               
                   
                   
                 YCATPTNSGYYGPYYYYGMDVW 
                 GGTKVEIK 
               
               
                   
                   
                 GQGTTVTVSS 
                   
               
               
                   
               
               
                 G5 
                 G5-P1- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 E05 
                 ASGYTFTSYNMHWVRQAPGQGLE 
                 SQGISNYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWINPNSGGTNYAQKFQGRV 
                 YASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQTYMMPYTF 
               
               
                   
                   
                 VYYCARDVMDVWGQGTTVTVSS 
                 GQGTKVEIK 
               
               
                   
               
               
                 G5 
                 G5- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P3G01 
                 ASGGTFSGYLVSWVRQAPGQGLE 
                 SQSISSYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWINPNSGGTNTAQKFQGRVT 
                 GASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 MTRDTSTSTVYMELSSLRSEDTAV 
                 ISSLQPEDFATYYCQQSYITPWTFG 
               
               
                   
                   
                 YYCAREGYGMDVWGQGTTVTVS 
                 QGTKVEIK 
               
               
                   
                   
                 S 
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P3G08 
                 ASGYIFRNYPMHWVRQAPGQGLE 
                 SQGISNYLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWINPDSGGTKYAQKFQGRV 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQSYITPYTFG 
               
               
                   
                   
                 VYYCARDNGVGVDYWGQGTLVT 
                 QGTKLEIK 
               
               
                   
                   
                 VSS 
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIVMTQSPDSLAVSLGERATINCK 
               
               
                   
                 P4B02 
                 ASGYTFTGYYMHWVRQAPGQGL 
                 TSQSVLYRPNNENYLAWYQQKPG 
               
               
                   
                   
                 EWMGWMNPNIGNTGYAQKFQGR 
                 QPPKLLIYQASIREPGVPDRFSGSG 
               
               
                   
                   
                 VTMTRDTSTSTVYMELSSLRSEDT 
                 SGTDFTLTISSLQAEDVAVYYCQQ 
               
               
                   
                   
                 AVYYCARGIADSGSYYGNGRDYY 
                 YYTTPYTFGQGTKLEIK 
               
               
                   
                   
                 YGMDVWGQGTTVTVSS 
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P4E04 
                 ASGGTFSSYGISWVRQAPGQGLE 
                 SQSISRFLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWINPNSGVTKYAQKFQGRV 
                 GASRPQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQSYSTPLTFG 
               
               
                   
                   
                 VYYCARGDYYFDYWGQGTLVTV 
                 QGTKVEIK 
               
               
                   
                   
                 SS 
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIVMTQSPLSLPVTPGEPASISCRSS 
               
               
                   
                 P1D06 
                 ASGYTFTSYDINWVRQAPGQGLE 
                 QSLLHSNGYNYLDWYLQKPGQSP 
               
               
                   
                   
                 WMGWINPNSGDTKYSQKFQGRVT 
                 QLLIYLGSHRASGVPDRFSGSGSGT 
               
               
                   
                   
                 MTRDTSTSTVYMELSSLRSEDTAV 
                 DFTLKISRVEAEDVGVYYCMQAL 
               
               
                   
                   
                 YYCARDGTRYYGMDVWGQGTTV 
                 QTPLTFGGGTKVEIK 
               
               
                   
                   
                 TVSS 
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 EVQLLESGGGLVKPGGSLRLSCAA 
                 EIVMTQSPATLSVSPGERATLSCRA 
               
               
                   
                 P1H11 
                 SGFTFSDYYMSWVRQAPGKGLEW 
                 SQSVSSNLAWYQQKPGQAPRLLIY 
               
               
                   
                   
                 VSYISSSSSYTNYADSVKGRFTISR 
                 AASARASGIPARFSGSGSGTEFTLT 
               
               
                   
                   
                 DDSKNTLYLQMNSLKTEDTAVYY 
                 ISSLQSEDFAVYYCQQYGSWPRTF 
               
               
                   
                   
                 CARDVVANFDYWGQGTLVTVSS 
                 GQGTKVEIK 
               
               
                   
               
               
                 G5 
                 G5R4- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P2B10 
                 ASGGTFSSYAISWVRQAPGQGLE 
                 SQSISSYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWMNPDSGSTGYAQRFQGRV 
                 GASRLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQSYSTPVTFG 
               
               
                   
                   
                 VYYCARGHSSGWYYYYGMDVW 
                 QGTKVEIK 
               
               
                   
                   
                 GQGTTVTVSS 
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 EVQLLESGGGLVQPGGSLRLSCAA 
                 DIVMTQSPLSLPVTPGEPASISCRSS 
               
               
                   
                 P2H8 
                 SGFTFTSYSMHWVRQAPGKGLEW 
                 QSLLHSNGYNYLDWYLQKPGQSP 
               
               
                   
                   
                 VSSITSFTNTMYYADSVKGRFTISR 
                 QLLIYLGSNRASGVPDRFSGSGSGT 
               
               
                   
                   
                 DNSKNTLYLQMNSLRAEDTAVYY 
                 DFTLKISRVEAEDVGVYYCMQAL 
               
               
                   
                   
                 CAKDLGSYGGYYWGQGTLVTVSS 
                 QTPYTFGQGTKVEIK 
               
               
                   
               
               
                 G5 
                 G5R4- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCQA 
               
               
                   
                 P3G05 
                 ASGYTFTNYYMHWVRQAPGQGL 
                 SEDISNHLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 EWMGIINPSGGSTSYAQKFQGRVT 
                 DALSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 MTRDTSTSTVYMELSSLRSEDTAV 
                 ISSLQPEDFATYYCQQANSFPFTFG 
               
               
                   
                   
                 YYCARSWFGGFNYHYYGMDVWG 
                 PGTKVDIK 
               
               
                   
                   
                 QGTTVTVSS 
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIVMTQSPLSLPVTPGEPASISCRSS 
               
               
                   
                 P4A07 
                 ASGYTFTSYYMHWVRQAPGQGLE 
                 QSLLHSNGYNYLDWYLQKPGQSP 
               
               
                   
                   
                 WMGWMNPNSGNTGYAQKFQGR 
                 QLLIYLGSNRASGVPDRFSGSGSGT 
               
               
                   
                   
                 VTMTRDTSTSTVYMELSSLRSEDT 
                 DFTLKISRVEAEDVGVYYCMQAL 
               
               
                   
                   
                 AVYYCARELPIGYGMDVWGQGTT 
                 QTPLTFGQGTKVEIK 
               
               
                   
                   
                 VTVSS 
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 QVQLVQSGAEVKKPGSSVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P4B01 
                 ASGGTFSSYAISWVRQAPGQGLE 
                 SQSISSYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGGIIPIVGTANYAQKFQGRVTI 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TADESTSTAYMELSSLRSEDTAVY 
                 ISSLQPEDFATYYCQQSYSTPLTFG 
               
               
                   
                   
                 YCARGGSYYYYGMDVWGQGTTV 
                 GGTKVEIK 
               
               
                   
                   
                 TVSS 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 CDR sequences of identified scFvs to G5, numbered according to the 
               
               
                 Kabat numbering scheme 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
                   
                   
                   
                   
               
               
                 group name 
                 HCDR1 
                 HCDR2 
                 HCDR3 
                 LCDR1 
                 LCDR2 
                 LCDR3 
               
               
                   
               
               
                 G5 
                 G5_P7_ 
                 YTFTS 
                 GIINPRS 
                 CARDGVR 
                 RSSQSLLH 
                 LGSYR 
                 CMQGLQ 
               
               
                   
                 E7 
                 YDIN 
                 GSTKYA 
                 YYGMDV 
                 SNGYNYL 
                 AS 
                 TPITF 
               
               
                   
                   
                   
                   
                 W 
                 D 
                   
                   
               
               
                   
               
               
                 G5 
                 G5_P7_ 
                 YTFTS 
                 GWMNP 
                 CARGVRG 
                 RSSQSLLH 
                 LGSSR 
                 CMQALQ 
               
               
                   
                 B3 
                 HDIN 
                 NSGDTG 
                 YDRSAGY 
                 SNGYNYL 
                 AS 
                 TPPTF 
               
               
                   
                   
                   
                 YA 
                 W 
                 D 
                   
                   
               
               
                   
               
               
                 G5 
                 G5_P7_ 
                 FSFSSY 
                 SYISGDS 
                 CASHDYG 
                 QASQDISN 
                 AASSL 
                 CQQAISF 
               
               
                   
                 A5 
                 WMS 
                 GYTNYA 
                 DYGEYFQ 
                 YLN 
                 QS 
                 PLTF 
               
               
                   
                   
                   
                   
                 HW 
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5_P7_ 
                 FTFSNS 
                 AYISSGS 
                 CARVSWY 
                 RASQSISS 
                 SASTLQ 
                 CQQANS 
               
               
                   
                 F6 
                 DMN 
                 STIYYA 
                 CSSTSCGV 
                 WLA 
                 S 
                 FPLTF 
               
               
                   
                   
                   
                   
                 NWFDPW 
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 FTFSNS 
                 ASISSSG 
                 CAKVNW 
                 RASQSISS 
                 AASSL 
                 CQQANS 
               
               
                   
                 P1B12 
                 DMN 
                 GYINYA 
                 NDGPYFD 
                 WLA 
                 QS 
                 FPLTF 
               
               
                   
                   
                   
                   
                 YW 
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 GTFSNF 
                 GGIIPILG 
                 CATPTNS 
                 RASQSISS 
                 AASTL 
                 CQQSYSI 
               
               
                   
                 P1C12 
                 GVS 
                 TANYA 
                 GYYGPYY 
                 WLA 
                 QS 
                 PLTF 
               
               
                   
                   
                   
                   
                 YYGMDV 
                   
                   
                   
               
               
                   
                   
                   
                   
                 W 
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5-P1- 
                 YTFTS 
                 GWINPN 
                 CARDVM 
                 RASQGISN 
                 YASSL 
                 CQQTYM 
               
               
                   
                 E05 
                 YNMH 
                 SGGTNY 
                 DVW 
                 YLN 
                 QS 
                 MPYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 GTFSG 
                 GWINPN 
                 CAREGYG 
                 RASQSISS 
                 GASSL 
                 CQQSYIT 
               
               
                   
                 P3G01 
                 YLVS 
                 SGGTNT 
                 MDVW 
                 YLN 
                 QS 
                 PWTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 YIFRNY 
                 GWINPD 
                 CARDNGV 
                 RASQGISN 
                 AASSL 
                 CQQSYIT 
               
               
                   
                 P3G08 
                 PMH 
                 SGGTKY 
                 GVDYW 
                 YLA 
                 QS 
                 PYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 YTFTG 
                 GWMNP 
                 CARGIAD 
                 KTSQSVL 
                 QASIRE 
                 CQQYYT 
               
               
                   
                 P4B02 
                 YYMH 
                 NIGNTG 
                 SGSYYGN 
                 YRPNNEN 
                 P 
                 TPYTF 
               
               
                   
                   
                   
                 YA 
                 GRDYYYG 
                 YLA 
                   
                   
               
               
                   
                   
                   
                   
                 MDVW 
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5- 
                 GTFSSY 
                 GWINPN 
                 CARGDYY 
                 RASQSISR 
                 GASRP 
                 CQQSYS 
               
               
                   
                 P4E04 
                 GIS 
                 SGVTKY 
                 FDYW 
                 FLN 
                 QS 
                 TPLTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 YTFTS 
                 GWINPN 
                 CARDGTR 
                 RSSQSLLH 
                 LGSHR 
                 CMQALQ 
               
               
                   
                 P1D06 
                 YDIN 
                 SGDTKY 
                 YYGMDV 
                 SNGYNYL 
                 AS 
                 TPLTF 
               
               
                   
                   
                   
                 S 
                 W 
                 D 
                   
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 FTFSDY 
                 SYISSSSS 
                 CARDVVA 
                 RASQSVSS 
                 AASAR 
                 CQQYGS 
               
               
                   
                 P1H11 
                 YMS 
                 YTNYA 
                 NFDYW 
                 NLA 
                 AS 
                 WPRTF 
               
               
                   
               
               
                 G5 
                 G5R4- 
                 GTFSSY 
                 GWMNP 
                 CARGHSS 
                 RASQSISS 
                 GASRL 
                 CQQSYS 
               
               
                   
                 P2B10 
                 AIS 
                 DSGSTG 
                 GWYYYY 
                 YLN 
                 QS 
                 TPVTF 
               
               
                   
                   
                   
                 YA 
                 GMDVW 
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 FTFTSY 
                 SSITSFTN 
                 CAKDLGS 
                 RSSQSLLH 
                 LGSNR 
                 CMQALQ 
               
               
                   
                 P2H8 
                 SMH 
                 TMYYA 
                 YGGYYW 
                 SNGYNYL 
                 AS 
                 TPYTF 
               
               
                   
                   
                   
                   
                   
                 D 
                   
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 YTFTN 
                 GIINPSG 
                 CARSWFG 
                 QASEDISN 
                 DALSL 
                 CQQANS 
               
               
                   
                 P3G05 
                 YYMH 
                 GSTSYA 
                 GFNYHYY 
                 HLN 
                 QS 
                 FPFTF 
               
               
                   
                   
                   
                   
                 GMDVW 
                   
                   
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 YTFTS 
                 GWMNP 
                 CARELPIG 
                 RSSQSLLH 
                 LGSNR 
                 CMQALQ 
               
               
                   
                 P4A07 
                 YYMH 
                 NSGNTG 
                 YGMDVW 
                 SNGYNYL 
                 AS 
                 TPLTF 
               
               
                   
                   
                   
                 YA 
                   
                 D 
                   
                   
               
               
                   
               
               
                 G5 
                 G5R4- 
                 GTFSSY 
                 GGIIPVM 
                 CARGGSY 
                 RASQSISS 
                 AASSL 
                 CQQSYS 
               
               
                   
                 P4B01 
                 AIS 
                 GTGNYA 
                 YYYGMD 
                 YLN 
                 QS 
                 TPLTF 
               
               
                   
                   
                   
                   
                 VW 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 VH and VL sequences of scFv hits that bind target G8 
               
            
           
           
               
               
               
            
               
                 Target 
                 Clone 
                   
               
            
           
           
               
               
               
               
            
               
                 group 
                 name 
                 V H   
                 V L   
               
               
                   
               
               
                 G8 
                 G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P1A03 
                 ASGGTFSRSAITWVRQAPGQGLE 
                 SQSITSYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWINPNSGATNYAQKFQGRV 
                 DASNLETGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQNYNSVTFG 
               
               
                   
                   
                 VYYCARDDYGDYVAYFQHWGQG 
                 QGTKLEIK 
               
               
                   
                   
                 TLVTVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCW 
               
               
                   
                 P1A04 
                 ASGYPFIGQYLHWVRQAPGQGLE 
                 ASQGISSYLAWYQQKPGKAPKLLI 
               
               
                   
                   
                 WMGIINPSGDSATYAQKFQGRVT 
                 YAASSLQSGVPSRFSGSGSGTDFTL 
               
               
                   
                   
                 MTRDTSTSTVYMELSSLRSEDTAV 
                 TISSLQPEDFATYYCQQSYNTPWT 
               
               
                   
                   
                 YYCARDLSYYYGMDVWGQGTTV 
                 FGPGTKVDIK 
               
               
                   
                   
                 TVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P1A06 
                 ASGYTFTNYYMHWVRQAPGQGL 
                 SQAISNSLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 EWMGWMNPIGGGTGYAQKFQGR 
                 AASTLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 VTMTRDTSTSTVYMELSSLRSEDT 
                 ISSLQPEDFATYYCGQSYSTPPTFG 
               
               
                   
                   
                 AVYYCARVYDFWSVLSGFDIWGQ 
                 QGTKLEIK 
               
               
                   
                   
                 GTLVTVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 EVQLLESGGGLVQPGGSLRLSCAA 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P1B03 
                 SGFTFSDYYMSWVRQAPGKGLEW 
                 SQSISSYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 VSGINWNGGSTGYADSVKGRFTIS 
                 KASSLESGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 RDNSKNTLYLQMNSLRAEDTAVY 
                 ISSLQPEDFATYYCQQSYSAPYTFG 
               
               
                   
                   
                 YCARVEQGYDIYYYYYMDVWGK 
                 PGTKVDIK 
               
               
                   
                   
                 GTTVTVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCQA 
               
               
                   
                 P1C11 
                 ASGGTLSSYPINWVRQAPGQGLE 
                 SQDISNYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWISTYSGHADYAQKLQGRV 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQSYSIPPTFG 
               
               
                   
                   
                 VYYCARSYDYGDYLNFDYWGQG 
                 GGTKVDIK 
               
               
                   
                   
                 TLVTVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 EVQLLESGGGLVQPGGSLRLSCAA 
                 DIQMTQSPSSLSASVGDRVTITCQA 
               
               
                   
                 P1D02 
                 SGFTFSSYWMSWVRQAPGKGLEW 
                 SQDISNYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 VSSISGRGDNTYYADSVKGRFTISR 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 DNSKNTLYLQMNSLRAEDTAVYY 
                 ISSLQPEDFATYYCQQSYSAPYTFG 
               
               
                   
                   
                 CARASGSGYYYYYGMDVWGQGT 
                 GGTKVEIK 
               
               
                   
                   
                 TVTVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P1H08 
                 ASGYTFGNYFMHWVRQAPGQGLE 
                 SQGINSYLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGMVNPSGGSETFAQKFQGRVT 
                 DASNLETGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 MTRDTSTSTVYMELSSLRSEDTAV 
                 ISSLQPEDFATYYCQQHNSYPPTFG 
               
               
                   
                   
                 YYCAASTWIQPFDYWGQGTLVTV 
                 QGTKLEIK 
               
               
                   
                   
                 SS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 EVQLLESGGGLVQPGGSLRLSCAA 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P2B05 
                 SGFDFSIYSMNWVRQAPGKGLEW 
                 SQSISRWLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 VSAISGSGGSTYYADSVKGRFTISR 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 DNSKNTLYLQMNSLRAEDTAVYY 
                 ISSLQPEDFATYYCQQYSTYPITIG 
               
               
                   
                   
                 CASNGNYYGSGSYYNYWGQGTL 
                 QGTKVEIK 
               
               
                   
                   
                 VTVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P2E06 
                 ASGYTLTTYYMHWVRQAPGQGLE 
                 SQGISNSLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWINPNSGGTNYAQKFQGRV 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQANSFPWTF 
               
               
                   
                   
                 VYYCARAVYYDFWSGPFDYWGQ 
                 GQGTKLEIK 
               
               
                   
                   
                 GTLVTVSS 
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P2C10 
                 ASGYTFTSYYMHWVRQAPGQGLE 
                 SQDVSTWLAWYQQKPGKAPKLLI 
               
               
                   
                   
                 WMGWINPYSGGTNYAQKFQGRV 
                 YAASSLQSGVPSRFSGSGSGTDFTL 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 TISSLQPEDFATYYCQQSHSTPQTF 
               
               
                   
                   
                 VYYCAKGGIYYGSGSYPSWGQGT 
                 GQGTKVEIK 
               
               
                   
                   
                 LVTVSS 
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 QVQLVQSGAEVKKPGSSVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P2E04 
                 ASGGTFSSYGVSWVRQAPGQGLE 
                 SQSISSWLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWISPYSGNTDYAQKFQGRVT 
                 DASNLETGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 ITADESTSTAYMELSSLRSEDTAVY 
                 ISSLQPEDFATYYCQQSYSTPLTFG 
               
               
                   
                   
                 YCARGLYYMDVWGKGTTVTVSS 
                 GGTKLEIK 
               
               
                   
               
               
                 G8 
                 R3G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P4F05 
                 ASGYTFSNMYLHWVRQAPGQGLE 
                 SQGISNYLAWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWINPNTGDTNYAQTFQGRV 
                 AASTLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQSYSTPLTFG 
               
               
                   
                   
                 VYYCARGLYGDYFLYYGMDVWG 
                 GGTKVEIK 
               
               
                   
                   
                 QGTKVTVSS 
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P5C03 
                 ASGYTFTSYYMHWVRQAPGQGLE 
                 SQGISNWLAWYQQKPGKAPKLLI 
               
               
                   
                   
                 WMGWMNPNSGNTGYAQKFQGR 
                 YAASTLQSGVPSRFSGSGSGTDFTL 
               
               
                   
                   
                 VTMTRDTSTSTVYMELSSLRSEDT 
                 TISSLQPEDFATYYCQQTYSTPWTF 
               
               
                   
                   
                 AVYYCARGLLGFGEFLTYGMDV 
                 GQGTKLEIK 
               
               
                   
                   
                 WGQGTLVTVSS 
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 EIVMTQSPATLSVSPGERATLSCRA 
               
               
                   
                 P5F02 
                 ASGYTFTGYYIHWVRQAPGQGLE 
                 SQSVGNSLAWYQQKPGQAPRLLIY 
               
               
                   
                   
                 WMGVINPSGGSTTYAQKLQGRVT 
                 GASTRATGIPARFSGSGSGTEFTLTI 
               
               
                   
                   
                 MTRDTSTSTVYMELSSLRSEDTAV 
                 SSLQSEDFAVYYCQQYGSSPYTFG 
               
               
                   
                   
                 YYCARDRDSSWTYYYYGMDVWG 
                 QGTKVEIK 
               
               
                   
                   
                 QGTTVTVSS 
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P5G08 
                 ASGYTFTSNYMHWVRQAPGQGLE 
                 SQSISGYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWMNPNSGNTGYAQKFQGR 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 VTMTRDTSTSTVYMELSSLRSEDT 
                 ISSLQPEDFATYYCQQSHSTPLTFG 
               
               
                   
                   
                 AVYYCARGLYGDYFLYYGMDVW 
                 QGTKVEIK 
               
               
                   
                   
                 GQGTTVTVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P1C01 
                 ASGGTFSSHAISWVRQAPGQGLE 
                 SQNIYTYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGVIIPSGGTSYTQKFQGRVTMT 
                 DASNLETGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 RDTSTSTVYMELSSLRSEDTAVYY 
                 ISSLQPEDFATYYCQQANGFPLTFG 
               
               
                   
                   
                 CARGDYYDSSGYYFPVYFDYWGQ 
                 GGTKVEIK 
               
               
                   
                   
                 GTLVTVSS 
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 QVQLVQSGAEVKKPGASVKVSCK 
                 DIQMTQSPSSLSASVGDRVTITCRA 
               
               
                   
                 P2C11 
                 ASGYTFTSYAMNWVRQAPGQGLE 
                 SQSISSYLNWYQQKPGKAPKLLIY 
               
               
                   
                   
                 WMGWINPNSGGTNYAQKFQGRV 
                 AASSLQSGVPSRFSGSGSGTDFTLT 
               
               
                   
                   
                 TMTRDTSTSTVYMELSSLRSEDTA 
                 ISSLQPEDFATYYCQQSYSTPLTFG 
               
               
                   
                   
                 VYYCARDPFWSGHYYYYGMDVW 
                 GGTKVEIK 
               
               
                   
                   
                 GQGTTVTVSS 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 CDR sequences of identified scFvs to G8, numbered according to the 
               
               
                 Kabat numbering scheme 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
                   
                   
                   
                   
               
               
                 group 
                 name 
                 HCDR1 
                 HCDR2 
                 HCDR3 
                 LCDR1 
                 LCDR2 
                 LCDR3 
               
               
                   
               
               
                 G8 
                 G8- 
                 GTFSRS 
                 GWINPN 
                 CARDDYG 
                 RASQSITS 
                 DASNL 
                 CQQNYN 
               
               
                   
                 P1A03 
                 AIT 
                 SGATNY 
                 DYVAYFQ 
                 YLN 
                 ET 
                 SVTF 
               
               
                   
                   
                   
                 A 
                 HW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 YPFIGQ 
                 GIINPSG 
                 CARDLSY 
                 WASQGISS 
                 AASSL 
                 CQQSYN 
               
               
                   
                 P1A04 
                 YLH 
                 DSATYA 
                 YYGMDV 
                 YLA 
                 QS 
                 TPWTF 
               
               
                   
                   
                   
                   
                 W 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 YTFTN 
                 GWMNPI 
                 CARVYDF 
                 RASQAISN 
                 AASTL 
                 CGQSYS 
               
               
                   
                 P1A06 
                 YYMH 
                 GGGTGY 
                 WSVLSGF 
                 SLA 
                 QS 
                 TPPTF 
               
               
                   
                   
                   
                 A 
                 DIW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 FTFSDY 
                 SGINWN 
                 CARVEQG 
                 RASQSISS 
                 KASSLE 
                 CQQSYS 
               
               
                   
                 P1B03 
                 YMS 
                 GGSTGY 
                 YDIYYYY 
                 YLN 
                 S 
                 APYTF 
               
               
                   
                   
                   
                 A 
                 YMDVW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 GTLSS 
                 GWISTYS 
                 CARSYDY 
                 QASQDISN 
                 AASSL 
                 CQQSYSI 
               
               
                   
                 P1C11 
                 YPIN 
                 GHADYA 
                 GDYLNFD 
                 YLN 
                 QS 
                 PPTF 
               
               
                   
                   
                   
                   
                 YW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 FTFSSY 
                 SSISGRG 
                 CARASGS 
                 QASQDISN 
                 AASSL 
                 CQQSYS 
               
               
                   
                 P1D02 
                 WMS 
                 DNTYYA 
                 GYYYYYG 
                 YLN 
                 QS 
                 APYTF 
               
               
                   
                   
                   
                   
                 MDVW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 YTFGN 
                 GMVNPS 
                 CAASTWI 
                 RASQGINS 
                 DASNL 
                 CQQHNS 
               
               
                   
                 P1H08 
                 YFMH 
                 GGSETFA 
                 QPFDYW 
                 YLA 
                 ET 
                 YPPTF 
               
               
                   
               
               
                 G8 
                 G8- 
                 FDFSIY 
                 SAISGSG 
                 CASNGNY 
                 RASQSISR 
                 AASSL 
                 CQQYST 
               
               
                   
                 P2B05 
                 SMN 
                 GSTYYA 
                 YGSGSYY 
                 WLA 
                 QS 
                 YPITI 
               
               
                   
                   
                   
                   
                 NYW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 YTLTT 
                 GWINPN 
                 CARAVYY 
                 RASQGISN 
                 AASSL 
                 CQQANS 
               
               
                   
                 P2E06 
                 YYMH 
                 SGGTNY 
                 DFWSGPF 
                 SLA 
                 QS 
                 FPWTF 
               
               
                   
                   
                   
                 A 
                 DYW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 YTFTS 
                 GWINPY 
                 CAKGGIY 
                 RASQDVS 
                 AASSL 
                 CQQSHS 
               
               
                   
                 P2C10 
                 YYMH 
                 SGGTNY 
                 YGSGSYP 
                 TWLA 
                 QS 
                 TPQTF 
               
               
                   
                   
                   
                 A 
                 SW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 GTFSSY 
                 GWISPYS 
                 CARGLYY 
                 RASQSISS 
                 DASNL 
                 CQQSYS 
               
               
                   
                 P2E04 
                 GVS 
                 GNTDYA 
                 MDVW 
                 WLA 
                 ET 
                 TPLTF 
               
               
                   
               
               
                 G8 
                 R3G8- 
                 YTFSN 
                 GWINPN 
                 CARGLYG 
                 RASQGISN 
                 AASTL 
                 CQQSYS 
               
               
                   
                 P4F05 
                 MYLH 
                 TGDTNY 
                 DYFLYYG 
                 YLA 
                 QS 
                 TPLTF 
               
               
                   
                   
                   
                 A 
                 MDVW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 YTFTS 
                 GWMNP 
                 CARGLLG 
                 RASQGISN 
                 AASTL 
                 CQQTYS 
               
               
                   
                 P5C03 
                 YYMH 
                 NSGNTG 
                 FGEFLTY 
                 WLA 
                 QS 
                 TPWTF 
               
               
                   
                   
                   
                 YA 
                 GMDVW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 YTFTG 
                 GVINPSG 
                 CARDRDS 
                 RASQSVG 
                 GASTR 
                 CQQYGS 
               
               
                   
                 P5F02 
                 YYIH 
                 GSTTYA 
                 SWTYYYY 
                 NSLA 
                 AT 
                 SPYTF 
               
               
                   
                   
                   
                   
                 GMDVW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 R3G8- 
                 YTFTS 
                 GWMNP 
                 CARGLYG 
                 RASQSISG 
                 AASSL 
                 CQQSHS 
               
               
                   
                 P5G08 
                 NYMH 
                 NSGNTG 
                 DYFLYYG 
                 YLN 
                 QS 
                 TPLTF 
               
               
                   
                   
                   
                 YA 
                 MDVW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 GTFSSH 
                 GVIIPSG 
                 CARGDYY 
                 RASQNIYT 
                 DASNL 
                 CQQANG 
               
               
                   
                 P1C01 
                 AIS 
                 GTSYT 
                 DSSGYYF 
                 YLN 
                 ET 
                 FPLTF 
               
               
                   
                   
                   
                   
                 PVYFDYW 
                   
                   
                   
               
               
                   
               
               
                 G8 
                 G8- 
                 YTFTS 
                 GWINPN 
                 CAKDPFW 
                 RASQSISS 
                 AASSL 
                 CQQSYS 
               
               
                   
                 P2C11 
                 YAMN 
                 SGGTNY 
                 SGHYYYY 
                 YLN 
                 QS 
                 TPLTF 
               
               
                   
                   
                   
                 A 
                 GMDVW 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 VH and VL sequences of scFv hits that bind target G10 
               
            
           
           
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
               
               
                 group 
                 name 
                 V H   
                 V L   
               
               
                   
               
               
                 G10 
                 R3G10- 
                 EVQLLESGGGLVKPGGSLRLSCAAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P1A07 
                 GFTFSSYWMSWVRQAPGKGLEWVS 
                 QGISNYLAWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 GISARSGRTYYADSVKGRFTISRDDS 
                 SLQGGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 KNTLYLQMNSLKTEDTAVYYCARDQ 
                 QPEDFATYYCQQYFTTPYTFGQGTKL 
               
               
                   
                   
                 DTIFGVVITWFDPWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P1B07 
                 GYTFTSYYMHWVRQAPGQGLEWMG 
                 QSISRWLAWYQQKPGKAPKLLIFDAS 
               
               
                   
                   
                 IIHPGGGTTSYAQKFQGRVTMTRDTS 
                 RLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 TSTVYMELSSLRSEDTAVYYCARDKV 
                 QPEDFATYYCQQAEAFPYTFGQGTK 
               
               
                   
                   
                 YGDGFDPWGQGTLVTVSS 
                 VEIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P1E12 
                 GYIFTGYYMHWVRQAPGQGLEWMG 
                 QSISSYLNWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 MIGPSDGSTSYAQKFQGRVTMTRDT 
                 SLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STSTVYMELSSLRSEDTAVYYCARED 
                 QPEDFATYYCQQSYSTPITFGQGTRL 
               
               
                   
                   
                 DSMDVWGKGTTVTVSS 
                 EIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P1F06 
                 GYTFIGYYMHWVRQAPGQGLEWMG 
                 QSISNYLNWYQQKPGKAPKLLIYKAS 
               
               
                   
                   
                 MIGPSDGSTSYAQKFQGRVTMTRDT 
                 SLESGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STSTVYMELSSLRSEDTAVYYCARDS 
                 QPEDFATYYCQQSYIIPYTFGQGTKL 
               
               
                   
                   
                 SGLDPWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P1H01 
                 GYTFTGYYMHWVRQAPGQGLEWMG 
                 QSISNYLNWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 MIGPSDGSTSYAQKFQGRVTMTRDT 
                 SLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STSTVYMELSSLRSEDTAVYYCARGV 
                 QPEDFATYYCHQTYSTPLTFGQGTKV 
               
               
                   
                   
                 GNLDYWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P1H08 
                 GVTFSTSAISWVRQAPGQGLEWMG 
                 QGISNYLAWYQQKPGKAPKWYSAS 
               
               
                   
                   
                 WISPYNGNTDYAQMLQGRVTMTRDT 
                 NLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STSTVYMELSSLRSEDTAVYYCARDA 
                 QPEDFATYYCQQAYSFPVVTFGQGTK 
               
               
                   
                   
                 HQYYDFWSGYYSGTYYYGMDVWGQ 
                 VEIK 
               
               
                   
                   
                 GTTVTVSS 
                   
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P2C04 
                 GGTFSNSIINWVRQAPGQGLEWMG 
                 QNISSYLNWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 WMNPNSGNTNYAQKFQGRVTMTRD 
                 SLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 TSTSTVYMELSSLRSEDTAVYYCARE 
                 QPEDFATYYCQQGYSTPLTFGQGTR 
               
               
                   
                   
                 QWPSYWYFDLWGRGTLVTVSS 
                 LEIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P2G11 
                 GGTFSTHDINWVRQAPGQGLEWMG 
                 QDISRYLAWYQQKPGKAPKLLIYDAS 
               
               
                   
                   
                 VINPSGGSAIYAQKFQGRVTMTRDTS 
                 NLETGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 TSTVYMELSSLRSEDTAVYYCARDRG 
                 QPEDFATYYCQQANSFPRTFGQGTK 
               
               
                   
                   
                 YSYGYFDYWGQGTLVTVSS 
                 VEIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCQAS 
               
               
                   
                 P3E04 
                 GNTFIGYYVHWVRQAPGQGLEWVGII 
                 QDISNYLNWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 NPNGGSISYAQKFQGRVTMTRDTST 
                 NLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STVYMELSSLRSEDTAVYYCARGSG 
                 QPEDFATYYCQQANSLPYTFGQGTK 
               
               
                   
                   
                 DPNYYYYYGLDVWGQGTTVTVSS 
                 VEIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P4A02 
                 GYTLSYYYMHWVRQAPGQGLEWMG 
                 QSISSYLNWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 MIGPSDGSTSYAQRFQGRVTMTRDT 
                 TLQNGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STGTVYMELSSLRSEDTAVYYCARDT 
                 QPEDFATYYCQQSYSTPFTFGPGTK 
               
               
                   
                   
                 GDHFDYWGQGTLVTVSS 
                 VDIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P4C05 
                 GYTFTGYYMHWVRQAPGQGLEWMG 
                 QRISSYLNWYQQKPGKAPKWYSAS 
               
               
                   
                   
                 IIGPSDGSTTYAQKFQGRVTMTRDTS 
                 TLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 TSTVYMELSSLRSEDTAVYYCARAEN 
                 QPEDFATYYCQQSYSTPFTFGPGTK 
               
               
                   
                   
                 GMDVWGQGTTVTVSS 
                 VDIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P4D04 
                 GYTFTGYYVHWVRQAPGQGLEWMG 
                 QSISSYLAWYQQKPGKAPKLLIYDAS 
               
               
                   
                   
                 IIAPSDGSTNYAQKFQGRVTMTRDTS 
                 KLETGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 TSTVYMELSSLRSEDTAVYYCARDPG 
                 QPEDFATYYCQQSYGVPTFGQGTKL 
               
               
                   
                   
                 GYMDVWGKGTTVTVSS 
                 EIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P4D10 
                 GYTFTGYYLHWVRQAPGQGLEWMG 
                 QGISSWLAWYQQKPGKAPKLLIYDAS 
               
               
                   
                   
                 MIGPSDGSTSYAQKFQGRVTMTRDT 
                 NLETGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STSTVYMELSSLRSEDTAVYYCARDG 
                 QPEDFATYYCQQSYSTPLTFGGGTK 
               
               
                   
                   
                 DAFDIWGQGTMVTVSS 
                 VEIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGSSVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P4E07 
                 GYTFTGYYMHWVRQAPGQGLEWMG 
                 QSISSYLNWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 RISPSDGSTTYAPKFQGRVTITADEST 
                 SLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STAYMELSSLRSEDTAVYYCARDMG 
                 QPEDFATYYCQQSYSTPLTFGGGTK 
               
               
                   
                   
                 DAFDIWGQGTTVTVSS 
                 VEIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P4E12 
                 GYTFTGYYMHWVRQAPGQGLEWMG 
                 QGISTYLAWYQQKPGKAPKLLIYDAS 
               
               
                   
                   
                 MIGPSDGSTSYAQRFQGRVTMTRDT 
                 SLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STSTVYMELSSLRSEDTAVYYCAREE 
                 QPEDFATYYCQQYYSYPVVTFGQGTR 
               
               
                   
                   
                 DGMDVWGQGTTVTVSS 
                 LEIK 
               
               
                   
               
               
                 G10 
                 R3G10-  
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P4G06 
                 GYTLSYYYMHWVRQAPGQGLEWMG 
                 QSISSYLNWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 MIGPSDGSTSYAQRFQGRVTMTRDT 
                 TLQNGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STGTVYMELSSLRSEDTAVYYCARDT 
                 QPEDFATYYCQQSYSTPFTFGPGTK 
               
               
                   
                   
                 GDHFDYWGQGTLVTVSS 
                 VDIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGSSVKVSCKAS 
                 DIVMTQSPLSLPVTPGEPASISCRSSQ 
               
               
                   
                 P5A08 
                 GGTFNNFAISWVRQAPGQGLEWMG 
                 SLLHSNGYNYLDWYLQKPGQSPQLLI 
               
               
                   
                   
                 GIIPIFDATNYAQKFQGRVTFTADEST 
                 YLGSNRASGVPDRFSGSGSGTDFTL 
               
               
                   
                   
                 STAYMELSSLRSEDTAVYYCARGEYS 
                 KISRVEAEDVGVYYCMQTLKTPLSFG 
               
               
                   
                   
                 SGFFFVGWFDLWGRGTQVTVSS 
                 GGTKVEIK 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 QVQLVQSGAEVKKPGASVKVSCKAS 
                 DIQMTQSPSSLSASVGDRVTITCRAS 
               
               
                   
                 P5C08 
                 GYNFTGYYMHWVRQAPGQGLEWM 
                 QSISSYLNWYQQKPGKAPKLLIYAAS 
               
               
                   
                   
                 GIIAPSDGSTNYAQKFQGRVTMTRDT 
                 SLQSGVPSRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 STSTVYMELSSLRSEDTAVYYCARET 
                 QPEDFATYYCQQSYSTPLTFGGGTK 
               
               
                   
                   
                 GDDAFDIWGQGTMVTVSS 
                 VEIK 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 CDR sequences of identified scFvs to G10, numbered according to the Kabat 
               
               
                 numbering scheme 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
                   
                   
                   
                   
               
               
                 group 
                 name 
                 HCDR1 
                 HCDR2 
                 HCDR3 
                 LCDR1 
                 LCDR2 
                 LCDR3 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 FTFSSYW 
                 SGISARS 
                 CARDQDTI 
                 RASQGISN 
                 AASSLQ 
                 CQQYFTT 
               
               
                   
                 P1A07 
                 MS 
                 GRTYYA 
                 FGVVITWF 
                 YLA 
                 G 
                 PYTF 
               
               
                   
                   
                   
                   
                 DPW 
                   
                   
                   
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTFTSYY 
                 GIIHPGG 
                 CARDKVYG 
                 RASQSISR 
                 DASRLQ 
                 CQQAEAF 
               
               
                   
                 P1B07 
                 MH 
                 GTTSYA 
                 DGFDPW 
                 WLA 
                 S 
                 PYTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YIFTGYYM 
                 GMIGPSD 
                 CAREDDS 
                 RASQSISS 
                 AASSLQ 
                 CQQSYST 
               
               
                   
                 P1E12 
                 H 
                 GSTSYA 
                 MDVW 
                 YLN 
                 S 
                 PITF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTFIGYYM 
                 GMIGPSD 
                 CARDSSGL 
                 RASQSISN 
                 KASSLE 
                 CQQSYIIP 
               
               
                   
                 P1F06 
                 H 
                 GSTSYA 
                 DPW 
                 YLN 
                 S 
                 YTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTFTGYY 
                 GMIGPSD 
                 CARGVGNL 
                 RASQSISN 
                 AASSLQ 
                 CHQTYST 
               
               
                   
                 P1H01 
                 MH 
                 GSTSYA 
                 DYW 
                 YLN 
                 S 
                 PLTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 VTFSTSAI 
                 GWISPYN 
                 CARDAHQ 
                 RASQGISN 
                 SASNLQ 
                 CQQAYSF 
               
               
                   
                 P1H08 
                 S 
                 GNTDYA 
                 YYDFWSG 
                 YLA 
                 S 
                 PWTF 
               
               
                   
                   
                   
                   
                 YYSGTYYY 
                   
                   
                   
               
               
                   
                   
                   
                   
                 GMDVW 
                   
                   
                   
               
               
                   
               
               
                 G10 
                 R3G10- 
                 GTFSNSII 
                 GWMNPN 
                 CAREQWP 
                 RASQNISS 
                 AASSLQ 
                 CQQGYS 
               
               
                   
                 P2C04 
                 N 
                 SGNTNYA 
                 SYWYFDL 
                 YLN 
                 S 
                 TPLTF 
               
               
                   
                   
                   
                   
                 W 
                   
                   
                   
               
               
                   
               
               
                 G10 
                 R3G10- 
                 GTFSTHDI 
                 GVINPSG 
                 CARDRGY 
                 RASQDISR 
                 DASNLE 
                 CQQANS 
               
               
                   
                 P2G11 
                 N 
                 GSAIYA 
                 SYGYFDY 
                 YLA 
                 T 
                 FPRTF 
               
               
                   
                   
                   
                   
                 W 
                   
                   
                   
               
               
                   
               
               
                 G10 
                 R3G10- 
                 NTFIGYYV 
                 GIINPNG 
                 CARGSGD 
                 QASQDISN 
                 AASNLQ 
                 CQQANSL 
               
               
                   
                 P3E04 
                 H 
                 GSISYA 
                 PNYYYYYG 
                 YLN 
                 S 
                 PYTF 
               
               
                   
                   
                   
                   
                 LDVW 
                   
                   
                   
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTLSYYY 
                 GMIGPSD 
                 CARDTGD 
                 RASQSISS 
                 AASTLQ 
                 CQQSYST 
               
               
                   
                 P4A02 
                 MH 
                 GSTSYA 
                 HFDYW 
                 YLN 
                 N 
                 PFTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTFTGYY 
                 GIIGPSDG 
                 CARAENG 
                 RASQRISS 
                 SASTLQ 
                 CQQSYST 
               
               
                   
                 P4C05 
                 MH 
                 STTYA 
                 MDVW 
                 YLN 
                 S 
                 PFTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTFTGYY 
                 GIIAPSDG 
                 CARDPGG 
                 RASQSISS 
                 DASKLE 
                 CQQSYG 
               
               
                   
                 P4D04 
                 VH 
                 STNYA 
                 YMDVW 
                 YLA 
                 T 
                 VPTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTFTGYYL 
                 GMIGPSD 
                 CARDGDAF 
                 RASQGISS 
                 DASNLE 
                 CQQSYST 
               
               
                   
                 P4D10 
                 H 
                 GSTSYA 
                 DIW 
                 WLA 
                 T 
                 PLTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTFTGYY 
                 GRISPSD 
                 CARDMGD 
                 RASQSISS 
                 AASSLQ 
                 CQQSYST 
               
               
                   
                 P4E07 
                 MH 
                 GSTTYA 
                 AFDIW 
                 YLN 
                 S 
                 PLTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTFTGYY 
                 GMIGPSD 
                 CAREEDG 
                 RASQGIST 
                 DASSLQ 
                 CQQYYS 
               
               
                   
                 P4E12 
                 MH 
                 GSTSYA 
                 MDVW 
                 YLA 
                 S 
                 YPVVTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YTLSYYY 
                 GMIGPSD 
                 CARDTGD 
                 RASQSISS 
                 AASTLQ 
                 CQQSYST 
               
               
                   
                 P4G06 
                 MH 
                 GSTSYA 
                 HFDYW 
                 YLN 
                 N 
                 PFTF 
               
               
                   
               
               
                 G10 
                 R3G10- 
                 GTFNNFAI 
                 GGIIPIFD 
                 CARGEYSS 
                 RSSQSLLH 
                 LGSNRA 
                 CMQTLKT 
               
               
                   
                 P5A08 
                 S 
                 ATNYA 
                 GFFFVGWF 
                 SNGYNYLD 
                 S 
                 PLSF 
               
               
                   
                   
                   
                   
                 DLW 
                   
                   
                   
               
               
                   
               
               
                 G10 
                 R3G10- 
                 YNFTGYY 
                 GIIAPSDG 
                 CARETGDD 
                 RASQSISS 
                 AASSLQ 
                 CQQSYST 
               
               
                   
                 P5C08 
                 MH 
                 STNYA 
                 AFDIW 
                 YLN 
                 S 
                 PLTF 
               
               
                   
               
            
           
         
       
     
     Table 15 (CDR3 Sequences for G10 TCRs) 
     This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     Table 16: Full Length Alpha and Beta TCR Sequences (G10) 
     This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     Table 18: CDR3 Sequences for TCR Clonotypes Specific for HLA-PEPTIDE A*01:01 HSEVGLPVY 
     This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     Table 19: Full Length Alpha V(J) and Beta V(D)J Sequences of Identified TCR Clonotypes Specific for HLA-PEPTIDE A*01:01 HSEVGLPVY 
     This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety. 
     
       
         
           
               
             
               
                 TABLE 33 
               
             
            
               
                   
               
               
                 VH and VL sequences for G2 scFv Selective Binders, selective for 
               
               
                 HLA-PEPTIDE Target HLA-A*01:01 NTDNNLAVY. 
               
            
           
           
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
               
               
                 group 
                 name 
                 V H   
                 V L   
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2E07 
                 SCKASGGTFSSATISWVRQAP 
                 TCRASQSISTWLAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWIYPNSGGTVY 
                 KAPKLLIYAASSLRSGVPSRF 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCAATE 
                 TYYCQQSYNTPYTFGQGTKL 
               
               
                   
                   
                 WLGVWGQGTTVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 EVQLLQSGAEVKKPGSSVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2E03 
                 SCKASGGTFSSYAISWVRQAP 
                 TCRASQSISRWLAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWINPNSGGTISA 
                 KAPKLLIYAASTVQSGVPSRF 
               
               
                   
                   
                 PNFQGRVTMTRDTSTSTVYM 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 ELSSLRSEDTAVYYCARANW 
                 TYYCQQSYSTPYTFGQGTKL 
               
               
                   
                   
                 LDYWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 EVQLLESGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2A11 
                 SCKASGYTFTTYDLAWVRQA 
                 TCRASQDISRWLAWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWINPNSGGTN 
                 KAPKLLIYAASRLQAGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARA 
                 TYYCQQSYSTPYSFGQGTKLE 
               
               
                   
                   
                 NWLDYWGQGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2C06 
                 SCKSSGYSFDSYVVNWVRQA 
                 TCRASQTISSWLAWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWINPNSGGTN 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARD 
                 TYYCQQSYSTPFTFGPGTKVD 
               
               
                   
                   
                 WVLDYWGQGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1G01 
                 SCKASGYTFTSYGISWVRQAP 
                 TCRASQTISSWLAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWMNPNSGGTN 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARG 
                 TYYCQQSYGVPYTFGQGTKV 
               
               
                   
                   
                 EWLDYWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1C02 
                 SCKASGYTFTSYGISWVRQAP 
                 TCRASQSISNWLAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWINPNSGGTNY 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARGW 
                 TYYCQQSYSAPYTFGPGTKV 
               
               
                   
                   
                 ELGYWGQGTLVTVSS 
                 DIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQPSSLSASVGDRVTI 
               
               
                   
                 P1H01 
                 SCKASGYTFTRYTINWVRQA 
                 TCRASQSVGNWLAWYQQKP 
               
               
                   
                   
                 PGQGLEWMGWINPNSGGTN 
                 GKAPKLLIYGASSLQTGVPSR 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 FSGSGSGTDFTLTISSLQPEDF 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARD 
                 ATYYCQQSYSAPYTFGQGTK 
               
               
                   
                   
                 FVGYDDWGQGTLVTVSS 
                 VEIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1B12 
                 SCKASGYTFTSYGITWVRQAP 
                 TCRASQNIGNWLAWYQQKP 
               
               
                   
                   
                 GQGLEWMGWINPNSGGTNY 
                 GKAPKLLIYAASTLQTGVPSR 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 FSGSGSGTDFTLTISSLQPEDF 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARDY 
                 ATYYCQQSYSAPYSFGQGTK 
               
               
                   
                   
                 GDLDYWGQGTLVTVSS 
                 LEIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1B06 
                 SCKASGGTFSNYILSWVRQAP 
                 TCRASQSISRWLAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWINPDSGGTNY 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARGS 
                 TYYCQQSYSTPYTFGQGTKL 
               
               
                   
                   
                 YGMDVWGQGTTVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2H10 
                 SCKASGYSFTRYNMHWVRQ 
                 TCRASQSISSWLAWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWINPNSGGT 
                 KAPKLLIYGASSLQSGVPSRF 
               
               
                   
                   
                 NYAQKFQGRVTMTRDTSTST 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAR 
                 TYYCQQSYSVPYSFGQGTKL 
               
               
                   
                   
                 DGYSGLDVWGKGTTVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1H10 
                 SCKASGGTFSSYAISWVRQAP 
                 TCRASQSISKWLAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWINPNNGGTNY 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARDS 
                 TYYCQQSYSAPYTFGQGTKV 
               
               
                   
                   
                 GVGMDVWGQGTTVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2C11 
                 SCKASGGTFNNYAFSWVRQA 
                 TCRASQGISNYLAWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWINPNSGGTN 
                 KAPKLLIYAASTLQSGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARD 
                 TYYCQQSYSVPYSFGQGTKL 
               
               
                   
                   
                 GVAVASDYWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1C09 
                 SCKASGYTFSSYNMHWVRQ 
                 TCRASQTISNYLNWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWINGNTGGT 
                 KAPKLLIYAASNLQSGVPSRF 
               
               
                   
                   
                 NYAQKFQGRVTMTRDTSTST 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAR 
                 TYYCQQSYSTPQTFGQGTKV 
               
               
                   
                   
                 GVNVDDFDYWGQGTLVTVS 
                 EIK 
               
               
                   
                   
                 S 
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1A10 
                 SCKASGGTFSSYAFSWVRQA 
                 TCRASRDIGRAVGWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWINPDTGYTR 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARG 
                 TYYCQQLDSYPFTFGPGTKV 
               
               
                   
                   
                 DYTGNWYFDLWGRGTLVTV 
                 DIK 
               
               
                   
                   
                 SS 
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 EVQLLESGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1B10 
                 SCKASGYTFTSYGISWVRQAP 
                 TCRASQSISSWLAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWINPYSGGTNY 
                 KAPKLLIYAASTLQSGVPSRF 
               
               
                   
                   
                 AQKLQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARAN 
                 TYYCQQSYSSPYTFGPGTKV 
               
               
                   
                   
                 WLDYWGQGTLVTVSS 
                 DIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1D07 
                 SCKASGYTFTSYGISWVRQAP 
                 TCQASQDISNYLNWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWISAYNGYTNY 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 AQNLQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARDQ 
                 TYYCQQSYSTPLTFGGGTKLE 
               
               
                   
                   
                 FYGGNSGGHDYWGQGTLVT 
                 IK 
               
               
                   
                   
                 VSS 
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1E05 
                 SCKASGYTFTDYNMHWVRQ 
                 TCRASQSIGRWLAWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWMNPNSGGT 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 NYAQKFQGRVTMTRDTSTST 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAR 
                 TYYCQQSYSTPYSFGQGTKV 
               
               
                   
                   
                 E-EDYWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1D03 
                 SCKASGYTFTRYTINWVRQA 
                 TCRASQSISTWLAWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWINPNSGGAN 
                 KAPKLLIYAASTLQSGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARG 
                 TYYCQQSYSTPYTFAQGTKL 
               
               
                   
                   
                 DWFDPWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1G12 
                 SCKASGYTFTSYLMHWVRQA 
                 TCQASQDISNYLNWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWISPNSGGTNY 
                 KAPKLLIYGASRLQSGVPSRF 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARGD 
                 TYYCQQSYSTPYTFGQGTKL 
               
               
                   
                   
                 WFDPWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2H11 
                 SCKASGYTFSDYYVHWVRQ 
                 TCRASQSISSWLAWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWINPNSGGT 
                 KAPKLLIYAASTLQSGVPSRF 
               
               
                   
                   
                 NYAQKFQGRVTMTRDTSTST 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAR 
                 TYYCQQSYSTPFTFGPGTKVD 
               
               
                   
                   
                 GEWFDPWGQGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1C03 
                 SCKASGYTFTTYYMHWVRQ 
                 TCRASQSVSNWLAWYQQKP 
               
               
                   
                   
                 APGQGLEWMGWINPNSGGT 
                 GKAPKLLIYAASSLQSGVPSR 
               
               
                   
                   
                 NYAQKFQGRVTMTRDTSTST 
                 FSGSGSGTDFTLTISSLQPEDF 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAR 
                 ATYYCQQSYSTPTFGQGTKL 
               
               
                   
                   
                 SDWFDPWGQGTLVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1G07 
                 SCKASGGTFSNYAINWVRQA 
                 TCQASQDISNYLNWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWISPYSGGTNY 
                 KAPKLLIYAASTLQSGVPSRF 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARDS 
                 TYYCQQTYAIPLTFGGGTKVE 
               
               
                   
                   
                 GSYFDYWGQGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1F12 
                 SCKASGYTFTDYYMHWVRQ 
                 TCQASQDIGSWLAWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWIYPNTGGT 
                 KAPKLLIYATSSLQSGVPSRFS 
               
               
                   
                   
                 NYAQKFQGRVTMTRDTSTST 
                 GSGSGTDFTLTISSLQPEDFAT 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAR 
                 YYCQQSYSTPYTFGQGTKLEI 
               
               
                   
                   
                 DYGGYVDYWGQGTLVTVSS 
                 K 
               
               
                   
               
               
                 G2 
                 G2- 
                 EVQLLESGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1G03 
                 SCKASGYTFTSYAMNWVRQ 
                 TCRASQGISRWLAWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWMNPNSGGT 
                 KAPKLLIYAASTLQPGVPSRF 
               
               
                   
                   
                 KYAQKFQGRVTMTRDTSTST 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAR 
                 TYYCQQSYIAPFTFGPGTKVD 
               
               
                   
                   
                 EGPAALDVWGQGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2B08 
                 SCKASGYTLTSHLIHWVRQA 
                 TCRASQGISNYLAWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWINPNSGGTN 
                 KAPKLLIYAASRLESGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARE 
                 TYYCQQSYSIPLTFGGGTKVE 
               
               
                   
                   
                 RRSGMDVWGQGTTVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 EVQLLESGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2A10 
                 SCKASGYSFTDYIVHWVRQA 
                 TCRASQSISSYLNWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWINPYSGGTK 
                 KAPKLLIYGVSSLQSGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARV 
                 TYYCQQSYSNPTFGQGTKVEI 
               
               
                   
                   
                 LQEGMDVWGQGTLVTVSS 
                 K 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2D04 
                 SCKASGYTFSNFLINWVRQAP 
                 TCRASQSISSWVAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWINPNSGGTNY 
                 KAPKLLIYGASNLESGVPSRF 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCASERE 
                 TYYCQQSYSTPYSFGQGTKLE 
               
               
                   
                   
                 LPFDIWGQGTMVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1C06 
                 SCKASGYTFTDYQMFWVRQ 
                 TCRASQGISNYLAWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWINPNSGGT 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 NYAQKFQGRVTMTRDTSTST 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAK 
                 TYYCQQSYSDQWTFGQGTK 
               
               
                   
                   
                 GGGGYGMDVWGQGTTVTVS 
                 VEIK 
               
               
                   
                   
                 S 
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2A09 
                 SCKASGGTFSSYAISWVRQAP 
                 TCRASQSISRWLAWYQQKPG 
               
               
                   
                   
                 GQGLEWMGWINPNSGGTNY 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 AQKFQGRVTMTRDTSTSTVY 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 MELSSLRSEDTAVYYCAAMG 
                 TYYCQQSYLPPYSFGQGTKV 
               
               
                   
                   
                 IAVAGGMDVWGQGTLVTVS 
                 EIK 
               
               
                   
                   
                 S 
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1B08 
                 SCKASGYTFTNYHMHWVRQ 
                 TCRASQSISNWLAWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWIHPDSGGTS 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTYFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARN 
                 TYYCQQSYSSPYTFGQGTKLE 
               
               
                   
                   
                 WNLDYWGQGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1E03 
                 SCKASGYTFTGYYMHWVRQ 
                 TCRASQSISHYLNWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGWMNPNSGNT 
                 KAPKLLIYGASSLQSGVPSRF 
               
               
                   
                   
                 GYAQKFQGRVTMTRDTSTST 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAT 
                 TYYCQQSYTTPWTFGQGTRL 
               
               
                   
                   
                 YDDGMDVWGQGTTVTVSS 
                 EIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2A03 
                 SCKASGYTFTSYTVNWVRQA 
                 TCRASQSISSWLAWYQQKPG 
               
               
                   
                   
                 PGQGLEWMGWINPNSGGTK 
                 KAPKLLIYAASTLQSGVPSRF 
               
               
                   
                   
                 YAQNFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARG 
                 TYYCQQSYLPPYSFGQGTKLE 
               
               
                   
                   
                 GGGALDYWGQGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2F01 
                 SCKASGYTFTSYYMHWVRQ 
                 TCQASQDISNYLNWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGMINPRDDTT 
                 KAPKLLIYGASRLQSGVPSRF 
               
               
                   
                   
                 DYARDFQGRVTMTRDTSTST 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 VYMELSSLRSEDTAVYYCAL 
                 TYYCQEGITYTFGQGTKVEIK 
               
               
                   
                   
                 SGNYYGMDVWGQGTTVTVS 
                   
               
               
                   
                   
                 S 
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGASVKV 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1H11 
                 SCKASGYTFTNYYMHWVRQ 
                 TCQASQDISNYLNWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGMINPSGGGTS 
                 KAPKLLIYAASSLQSGVPSRF 
               
               
                   
                   
                 YAQKFQGRVTMTRDTSTSTV 
                 SGSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 YMELSSLRSEDTAVYYCARG 
                 TYYCQQYYSYPFTFGPGTKV 
               
               
                   
                   
                 NPWELRLDYWGQGTLVTVSS 
                 DIK 
               
               
                   
               
               
                 G2 
                 G2- 
                 QVQLVQSGAEVKKPGSSVKV 
                 EIVMTQSPATLSVSPGERATL 
               
               
                   
                 P1D06 
                 SCKASGYTFTSQYMHWVRQ 
                 SCRASQSVSRNLAWYQQKPG 
               
               
                   
                   
                 APGQGLEWMGRIIPLLGIVNY 
                 QAPRLLIYGASTRATGIPARFS 
               
               
                   
                   
                 AQKFQGRVTITADESTSTAY 
                 GSGSGTEFTLTISSLQSEDFAV 
               
               
                   
                   
                 MELSSLRSEDTAVYYCARDK 
                 YYCQHYGYSPVTFGQGTKLE 
               
               
                   
                   
                 NYYGMDVWGQGTTVTVSS 
                 IK 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 34 
               
             
            
               
                   
               
               
                 CDR sequences for G2 selective binders, selective for HLA-PEPTIDE 
               
               
                 Target HLA-A*01:01 NTDNNLAVY (determined according to Kabat 
               
               
                 numbering) 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
                   
                   
                   
                   
               
               
                 group 
                 name 
                 CDR-H1 
                 CDR-H2 
                 CDR-H3 
                 CDR-L1 
                 CDR-L2 
                 CDR-L3 
               
               
                   
               
               
                 G2 
                 G2- 
                 GTFSSA 
                 GWIYPN 
                 CAATE 
                 RASQSI 
                 AASSLR 
                 CQQSYN 
               
               
                   
                 P2E07 
                 TIS 
                 SGGTVY 
                 WLGVW 
                 STWLA 
                 S 
                 TPYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 GTFSSY 
                 GWINPN 
                 CARAN 
                 RASQSI 
                 AASTV 
                 CQQSYS 
               
               
                   
                 P2E03 
                 AIS 
                 SGGTIS 
                 WLDYW 
                 SRWLA 
                 QS 
                 TPYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTTY 
                 GWINPN 
                 CARAN 
                 RASQDI 
                 AASRLQ 
                 CQQSYS 
               
               
                   
                 P2A11 
                 DLA 
                 SGGTNY 
                 WLDYW 
                 SRWLA 
                 A 
                 TPYSF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YSFDSY 
                 GWINPN 
                 CARDW 
                 RASQTI 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P2C06 
                 VVN 
                 SGGTNY 
                 VLDYW 
                 SSWLA 
                 S 
                 TPFTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GWMNP 
                 CARGE 
                 RASQTI 
                 AASSLQ 
                 CQQSYG 
               
               
                   
                 P1G01 
                 GIS 
                 NSGGTN 
                 WLDYW 
                 SSWLA 
                 S 
                 VPYTF 
               
               
                   
                   
                   
                 YA 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GWINPN 
                 CARGW 
                 RASQSI 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P1C02 
                 GIS 
                 SGGTNY 
                 ELGYW 
                 SNWLA 
                 S 
                 APYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTRY 
                 GWINPN 
                 CARDFV 
                 RASQSV 
                 GASSLQ 
                 CQQSYS 
               
               
                   
                 P1H01 
                 TIN 
                 SGGTNY 
                 GYDDW 
                 GNWLA 
                 T 
                 APYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GWINPN 
                 CARDY 
                 RASQNI 
                 AASTLQ 
                 CQQSYS 
               
               
                   
                 P1B12 
                 GIT 
                 SGGTNY 
                 GDLDY 
                 GNWLA 
                 T 
                 APYSF 
               
               
                   
                   
                   
                 A 
                 W 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 GTFSNY 
                 GWINPD 
                 CARGSY 
                 RASQSI 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P1B06 
                 ILS 
                 SGGTNY 
                 GMDVW 
                 SRWLA 
                 S 
                 TPYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YSFTRY 
                 GWINPN 
                 CARDG 
                 RASQSI 
                 GASSLQ 
                 CQQSYS 
               
               
                   
                 P2H10 
                 NMH 
                 SGGTNY 
                 YSGLDV 
                 SSWLA 
                 S 
                 VPYSF 
               
               
                   
                   
                   
                 A 
                 W 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 GTFSSY 
                 GWINPN 
                 CARDSG 
                 RASQSI 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P1H10 
                 AIS 
                 NGGTN 
                 VGMDV 
                 SKWLA 
                 S 
                 APYTF 
               
               
                   
                   
                   
                 YA 
                 W 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 GTFNNY 
                 GWINPN 
                 CARDG 
                 RASQGI 
                 AASTLQ 
                 CQQSYS 
               
               
                   
                 P2C11 
                 AFS 
                 SGGTNY 
                 VAVAS 
                 SNYLA 
                 S 
                 VPYSF 
               
               
                   
                   
                   
                 A 
                 DYW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFSSY 
                 GWING 
                 CARGV 
                 RASQTI 
                 AASNL 
                 CQQSYS 
               
               
                   
                 P1C09 
                 NMH 
                 NTGGT 
                 NVDDF 
                 SNYLN 
                 QS 
                 TPQTF 
               
               
                   
                   
                   
                 NYA 
                 DYW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 GTFSSY 
                 GWINPD 
                 CARGD 
                 RASRDI 
                 AASSLQ 
                 CQQLDS 
               
               
                   
                 P1A10 
                 AFS 
                 TGYTRY 
                 YTGNW 
                 GRAVG 
                 S 
                 YPFTF 
               
               
                   
                   
                   
                 A 
                 YFDLW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GWINPY 
                 CARAN 
                 RASQSI 
                 AASTLQ 
                 CQQSYS 
               
               
                   
                 P1B10 
                 GIS 
                 SGGTNY 
                 WLDYW 
                 SSWLA 
                 S 
                 SPYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GWISAY 
                 CARDQF 
                 QASQDI 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P1D07 
                 GIS 
                 NGYTN 
                 YGGNS 
                 SNYLN 
                 S 
                 TPLTF 
               
               
                   
                   
                   
                 YA 
                 GGHDY 
                   
                   
                   
               
               
                   
                   
                   
                   
                 W 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTDY 
                 GWMNP 
                 CAREED 
                 RASQSI 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P1E05 
                 NMH 
                 NSGGTN 
                 GRWLA 
                 S 
                 TPYSF 
                   
               
               
                   
                   
                   
                 YA 
                 YW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTRY 
                 GWINPN 
                 CARGD 
                 RASQSI 
                 AASTLQ 
                 CQQSYS 
               
               
                   
                 P1D03 
                 TIN 
                 SGGAN 
                 WFDPW 
                 STWLA 
                 S 
                 TPYTF 
               
               
                   
                   
                   
                 YA 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GWISPN 
                 CARGD 
                 QASQDI 
                 GASRLQ 
                 CQQSYS 
               
               
                   
                 P1G12 
                 LMH 
                 SGGTNY 
                 WFDPW 
                 SNYLN 
                 S 
                 TPYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFSDY 
                 GWINPN 
                 CARGE 
                 RASQSI 
                 AASTLQ 
                 CQQSYS 
               
               
                   
                 P2H11 
                 YVH 
                 SGGTNY 
                 WFDPW 
                 SSWLA 
                 S 
                 TPFTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTTY 
                 GWINPN 
                 CARSD 
                 RASQSV 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P1C03 
                 YMH 
                 SGGTNY 
                 WFDPW 
                 SNWLA 
                 S 
                 TPTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 GTFSNY 
                 GWISPY 
                 CARDSG 
                 QASQDI 
                 AASTLQ 
                 CQQTY 
               
               
                   
                 P1G07 
                 AIN 
                 SGGTNY 
                 SYFDY 
                 SNYLN 
                 S 
                 AIPLTF 
               
               
                   
                   
                   
                 A 
                 W 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTDY 
                 GWIYPN 
                 CARDY 
                 QASQDI 
                 ATSSLQ 
                 CQQSYS 
               
               
                   
                 P1F12 
                 YMH 
                 TGGTN 
                 GGYVD 
                 GSWLA 
                 S 
                 TPYTF 
               
               
                   
                   
                   
                 YA 
                 YW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GWMNP 
                 CAREGP 
                 RASQGI 
                 AASTLQ 
                 CQQSYI 
               
               
                   
                 P1G03 
                 AMN 
                 NSGGTK 
                 AALDV 
                 SRWLA 
                 P 
                 APFTF 
               
               
                   
                   
                   
                 YA 
                 W 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTLTSH 
                 GWINPN 
                 CARERR 
                 RASQGI 
                 AASRLE 
                 CQQSYS 
               
               
                   
                 P2B08 
                 LIH 
                 SGGTNY 
                 SGMDV 
                 SNYLA 
                 S 
                 IPLTF 
               
               
                   
                   
                   
                 A 
                 W 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YSFTDY 
                 GWINPY 
                 CARVL 
                 RASQSI 
                 GVSSLQ 
                 CQQSYS 
               
               
                   
                 P2A10 
                 IVH 
                 SGGTKY 
                 QEGMD 
                 SSYLN 
                 S 
                 NPTF 
               
               
                   
                   
                   
                 A 
                 VW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFSNF 
                 GWINPN 
                 CASERE 
                 RASQSI 
                 GASNLE 
                 CQQSYS 
               
               
                   
                 P2D04 
                 LIN 
                 SGGTNY 
                 LPFDIW 
                 SSWVA 
                 S 
                 TPYSF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTDY 
                 GWINPN 
                 CAKGG 
                 RASQGI 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P1C06 
                 QMF 
                 SGGTNY 
                 GGYGM 
                 SNYLA 
                 S 
                 DQWTF 
               
               
                   
                   
                   
                 A 
                 DVW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 GTFSSY 
                 GWINPN 
                 CAAMGI 
                 RASQSI 
                 AASSLQ 
                 CQQSYL 
               
               
                   
                 P2A09 
                 AIS 
                 SGGTNY 
                 AVAGG 
                 SRWLA 
                 S 
                 PPYSF 
               
               
                   
                   
                   
                 A 
                 MDVW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTNY 
                 GWIHPD 
                 CARNW 
                 RASQSI 
                 AASSLQ 
                 CQQSYS 
               
               
                   
                 P1B08 
                 HMH 
                 SGGTSY 
                 NLDYW 
                 SNWLA 
                 S 
                 SPYTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTGY 
                 GWMNP 
                 CATYD 
                 RASQSI 
                 GASSLQ 
                 CQQSYT 
               
               
                   
                 P1E03 
                 YMH 
                 NSGNTG 
                 DGMDV 
                 SHYLN 
                 S 
                 TPWTF 
               
               
                   
                   
                   
                 YA 
                 W 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GWINPN 
                 CARGG 
                 RASQSI 
                 AASTLQ 
                 CQQSYL 
               
               
                   
                 P2A03 
                 TVN 
                 SGGTKY 
                 GGALD 
                 SSWLA 
                 S 
                 PPYSF 
               
               
                   
                   
                   
                 A 
                 YW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSY 
                 GMINPR 
                 CALSGN 
                 QASQDI 
                 GASRLQ 
                 CQEGIT 
               
               
                   
                 P2F01 
                 YMH 
                 DDTTD 
                 YYGMD 
                 SNYLN 
                 S 
                 YTF 
               
               
                   
                   
                   
                 YA 
                 VW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTNY 
                 GMINPS 
                 CARGNP 
                 QASQDI 
                 AASSLQ 
                 CQQYYS 
               
               
                   
                 P1H11 
                 YMH 
                 GGGTSY 
                 WELRL 
                 SNYLN 
                 S 
                 YPFTF 
               
               
                   
                   
                   
                 A 
                 DYW 
                   
                   
                   
               
               
                   
               
               
                 G2 
                 G2- 
                 YTFTSQ 
                 GRIIPLL 
                 CARDK 
                 RASQSV 
                 GASTRA 
                 CQHYG 
               
               
                   
                 P1D06 
                 YMH 
                 GIVNYA 
                 NYYGM 
                 SRNLA 
                 T 
                 YSPVTF 
               
               
                   
                   
                   
                   
                 DVW 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 35 
               
             
            
               
                   
               
               
                 VH and VL sequences for scFv selective binders 
               
               
                 selective for HLA-PEPTIDE Target HLA-A*02:01 LLASSILCA. 
               
            
           
           
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
               
               
                 group 
                 name 
                 VH 
                 VL 
               
               
                   
               
               
                 G7 
                 G7R3- 
                 QVQLVQSGAEVKKPGASVKVS 
                 EIVMTQSPATLSVSPGERATL 
               
               
                   
                 P1C6 
                 CKASGGTFSNYGISWVRQAPG 
                 SCRASQSVSSSNLAWYQQKPG 
               
               
                   
                   
                 QGLEWMGIINPGGSTSYAQKF 
                 QAPRLLIYGASTRATGIPARF 
               
               
                   
                   
                 QGRVTMTRDTSTSTVYMELSS 
                 SGSGSGTEFTLTISSLQSEDF 
               
               
                   
                   
                 LRSEDTAVYYCARDGYDFWSG 
                 AVYYCHHYGRSHTFGQGTKVE 
               
               
                   
                   
                 YTSDDYWGQGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G7 
                 G7R3- 
                 EVQLLESGGGLVQPGGSLRLS 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1G10 
                 CAASGFTFSSYAMHWVRQAPG 
                 TCRASQDIRNDLGWYQQKPGK 
               
               
                   
                   
                 KGLEWVSGISGSGGSTYYADS 
                 APKLLIYAASSLQSGVPSRFS 
               
               
                   
                   
                 VKGRFTISRDNSKNTLYLQMN 
                 GSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 SLRAEDTAVYYCASDYGDYRG 
                 TYYCQQANAFPPTFGQGTKVE 
               
               
                   
                   
                 QGTLVTVSS 
                 IK 
               
               
                   
               
               
                 G7 
                 1-G7R3- 
                 QVQLVQSGAEVKKPGASVKVS 
                 DIVMTQSPDSLAVSLGERATI 
               
               
                   
                 P1B4  
                 CKASGYTFSNYYIHWVRQAPG 
                 NCKSSQSVFYSSNNKNQLAWY 
               
               
                   
                   
                 QGLEWMGWLNPNSGNTGYAQR 
                 QQKPGQPPKLLIYWASTRESG 
               
               
                   
                   
                 FQGRVTMTRDTSTSTVYMELS 
                 VPDRFSGSGSGTDFTLTISSL 
               
               
                   
                   
                 SLRSEDTAVYYCARDLMTTVV 
                 QAEDVAVYYCQQYYSIPLTFG 
               
               
                   
                   
                 TPGDYGMDVWGQGTTVTVSS 
                 QGTKLEIK 
               
               
                   
               
               
                 G7 
                 2-G7R4- 
                 QVQLVQSGAEVKKPGASMKVS 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P2C2 
                 CKASGYTFTTDGISWVRQAPG 
                 TCQASQDIFKYLNWYQQKPGK 
               
               
                   
                   
                 QGLEWMGRIYPHSGYTEYAKK 
                 APKLLIYAASTLQSGVPSRFS 
               
               
                   
                   
                 FKGRVTMTRDTSTSTVYMELS 
                 GSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 SLRSEDTAVYYCARQDGGAFA 
                 TYYCQQSYSTPPTFGQGTRLE 
               
               
                   
                   
                 FDIWGQGTMVTVSS 
                 IK 
               
               
                   
               
               
                 G7 
                 3-G7R4- 
                 QVQLVQSGAEVKKPGASVKVS 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 P1A3 
                 CKASGYTFTSQYMHWVRQAPG 
                 TCRASQSISTWLAWYQQKPGK 
               
               
                   
                   
                 QGLEWMGWISPNNGDTNYAQK 
                 APKLLIYYASSLQSGVPSRFS 
               
               
                   
                   
                 FQGRVTMTRDTSTSTVYMELS 
                 GSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 SLRSEDTAVYYCARELGYYYG 
                 TYYCQQSYSFPYTFGQGTKVE 
               
               
                   
                   
                 MDVWGQGTTVTVSS 
                 IK 
               
               
                   
               
               
                 G7 
                 4-G7R4- 
                 QVQLVQSGAEVKKPGSSVKVS 
                 DIVMTQSPLSLPVTPGEPASI 
               
               
                   
                 B5-P2E9 
                 CKASRYTFTSYDINWVRQAPG 
                 SCSSSQSLLHSNGYNYLDWYL 
               
               
                   
                   
                 QGLEWMGRIIPMLNIANYAPK 
                 QKPGQSPQLLIYLGSNRASGV 
               
               
                   
                   
                 FQGRVTITADESTSTAYMELS 
                 PDRFSGSGSGTDFTLKISRVE 
               
               
                   
                   
                 SLRSEDTAVYYCARALIFGVP 
                 AEDVGVYYCMQALQTPLTFGG 
               
               
                   
                   
                 LLPYGMDVWGQGTTVTVSS 
                 GTKVEIK 
               
               
                   
               
               
                 G7 
                 5-G7R4- 
                 EVQLLQSGGGLVQPGGSLRLS 
                 DIQMTQSPSSLSASVGDRVTI 
               
               
                   
                 B10- 
                 CAASGFTFSSSWMHWVRQAPG 
                 TCQASQDISNYLNWYQQKPGK 
               
               
                   
                 P1F8 
                 KGLEWVSFISTSSGYIYYADS 
                 APKLLIYSASNLRSGVPSRFS 
               
               
                   
                   
                 VKGRFTISRDNSKNTLYLQMN 
                 GSGSGTDFTLTISSLQPEDFA 
               
               
                   
                   
                 SLRAEDTAVYYCAKDLATVGE 
                 TYYCQQGNTFPLTFGQGTKVE 
               
               
                   
                   
                 PYYYYGMDVWGQGTTVTVSS 
                 IK 
               
               
                   
               
               
                 G7 
                 B7 
                 QVQLVQSGAEVKKPGSSVKVS 
                 DIVMTQSPLSLPVTPGEPASI 
               
               
                   
                 (G7R3- 
                 CKASGDTFNTYALSWVRQAPG 
                 SCRSSQSLLHSNGYNYLDWYL 
               
               
                   
                 P3A9) 
                 QGLEWMGWMNPNSGNAGYAQK 
                 QKPGQSPQLLIYLGSNRASGV 
               
               
                   
                   
                 FQGRVTITADESTSTAYMELS 
                 PDRFSGSGSGTDFTLKISRVE 
               
               
                   
                   
                 SLRSEDTAVYYCARLWFGELH 
                 AEDVGVYYCMQGSHWPPSFGQ 
               
               
                   
                   
                 YYYYYGMDVWGQGTMVTVSS 
                 GTRLEIK 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 36 
               
             
            
               
                   
               
               
                 CDR sequences for G7 selective binders selective 
               
               
                 for HLA-PEPTIDE Target HLA-A*02:01 LLASSILCA 
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                 Target 
                 Clone 
                   
                   
                   
                   
                   
                   
               
               
                 group 
                 name 
                 CDR-H1 
                 CDR-H2 
                 CDR-H3 
                 CDR-L1 
                 CDR-L2 
                 CDR-L3 
               
               
                   
               
               
                 G7 
                 G7R3- 
                 GTFSNY 
                 GIINPG 
                 CARDGY 
                 RASQSV 
                 GASTRAT 
                 CHHYGR 
               
               
                   
                 PIC6 
                 GIS 
                 GSTSYA 
                 DFWSGY 
                 SSSNLA 
                   
                 SHTF 
               
               
                   
                   
                   
                   
                 TSDDYW 
                   
                   
                   
               
               
                   
               
               
                 G7 
                 G7R3- 
                 FTFSSY 
                 SGISGS 
                 CASDYG 
                 RASQDI 
                 AASSLQS 
                 CQQANA 
               
               
                   
                 P1G10 
                 AMH 
                 GGSTYY 
                 DYR 
                 RNDLG 
                   
                 FPPTF 
               
               
                   
                   
                   
                 A 
                   
                   
                   
                   
               
               
                   
               
               
                 G7 
                 1-G7R3- 
                 YTFSNY 
                 GWLNPN 
                 CARDLM 
                 KSSQSV 
                 WASTRES 
                 CQQYYS 
               
               
                   
                 P1B4 
                 YIH 
                 SGNTGY 
                 TTVVTP 
                 FYSSNN 
                   
                 IPLTF 
               
               
                   
                   
                   
                 A 
                 GDYGMD 
                 KNQLA 
                   
                   
               
               
                   
                   
                   
                   
                 VW 
                   
                   
                   
               
               
                   
               
               
                 G7 
                 2-G7R4- 
                 YTFTTD 
                 GRIYPH 
                 CARQDG 
                 QASQDI 
                 AASTLQS 
                 CQQSYS 
               
               
                   
                 P2C2 
                 GIS 
                 SGYTEY 
                 GAFAFD 
                 FKYLN 
                   
                 TPPTF 
               
               
                   
                   
                   
                 A 
                 IW 
                   
                   
                   
               
               
                   
               
               
                 G7 
                 3-G7R4- 
                 YTFTSQ 
                 GWISPN 
                 CARELG 
                 RASQSI 
                 YASSLQS 
                 CQQSYS 
               
               
                   
                 P1A3 
                 YMH 
                 NGDTNY 
                 YYYGMD 
                 STWLA 
                   
                 FPYTF 
               
               
                   
                   
                   
                 A 
                 VW 
                   
                   
                   
               
               
                   
               
               
                 G7 
                 4-G7R4- 
                 YTFTSY 
                 GRIIPM 
                 CARALI 
                 SSSQSL 
                 LGSNRAS 
                 CMQALQ 
               
               
                   
                 B5-P2E9 
                 DIN 
                 LNIANY 
                 FGVPLL 
                 LHSNGY 
                   
                 TPLTF 
               
               
                   
                   
                   
                 A 
                 PYGMDV 
                 NYLD 
                   
                   
               
               
                   
                   
                   
                   
                 W 
                   
                   
                   
               
               
                   
               
               
                 G7 
                 5-G7R4- 
                 FTFSSS 
                 SFISTS 
                 CAKDLA 
                 QASQDI 
                 SASNLRS 
                 CQQGNT 
               
               
                   
                 B10-P1F8 
                 WMH 
                 SGYIYY 
                 TVGEPY 
                 SNYLN 
                   
                 FPLTF 
               
               
                   
                   
                   
                 A 
                 YYYGMD 
                   
                   
                   
               
               
                   
                   
                   
                   
                 VW 
                   
                   
                   
               
               
                   
               
               
                 G7 
                 B7(G7R3- 
                 DTFNTY 
                 GWMNPN 
                 CARLWF 
                 RSSQSL 
                 LGSNRAS 
                 CMQGSH 
               
               
                   
                 P3A9) 
                 ALS 
                 SGNAGY 
                 GELHYY 
                 LHSNGY 
                   
                 WPPSF 
               
               
                   
                   
                   
                 A 
                 YYYGMD 
                 NYLD 
                   
                   
               
               
                   
                   
                   
                   
                 VW 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 38 
               
               
                   
               
               
                 amino acid sequences of selected HLA subtypes 
               
               
                 and B2MG (beta-2 microglobulin) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 A*01:01 
               
               
                 MGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRA 
               
               
                   
               
               
                 PWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMY 
               
               
                   
               
               
                 GCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEA 
               
               
                   
               
               
                 VHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEA 
               
               
                   
               
               
                 TLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVV 
               
               
                   
               
               
                 PSGEEQRYTCHVQHEGLPKPLTLR 
               
               
                   
               
               
                 A*02:01 
               
               
                 MGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVREDSDAASQRMEPRA 
               
               
                   
               
               
                 PWIEQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMY 
               
               
                   
               
               
                 GCDVGSDWRFLRGYHQYAYDGKDYIALKEDLRSWTAADMAAQTTKHKWEA 
               
               
                   
               
               
                 AHVAEQLRAYLEGTCVEWLRRYLENGKETLQRTDAPKTHMTHHAVSDHEA 
               
               
                   
               
               
                 TLRCWALSFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVV 
               
               
                   
               
               
                 PSGQEQRYTCHVQHEGLPKPLTLR 
               
               
                   
               
               
                 B*35:01 
               
               
                 MGSHSMRYFYTAMSRPGRGEPRFIAVGYVDDTQFVREDSDAASPRTEPRA 
               
               
                   
               
               
                 PWIEQEGPEYWDRNTQIFKTNTQTYRESLRNLRGYYNQSEAGSHIIQRMY 
               
               
                   
               
               
                 GCDLGPDGRLLRGHDQSAYDGKDYIALNEDLSSWTAADTAAQITQRKWEA 
               
               
                   
               
               
                 ARVAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPVSDHEA 
               
               
                   
               
               
                 TLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVV 
               
               
                   
               
               
                 PSGEEQRYTCHVQHEGLPKPLTLR 
               
               
                   
               
               
                 B2MG 
               
               
                 MIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKV 
               
               
                   
               
               
                 EHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM 
               
               
                   
               
            
           
         
       
     
     CDR3 and V(D)J Sequences of TCR Clonotypes Confirmed Through Resorting 
     These sequences are included in PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     CDR3 and V(D)J Sequences of TCR Clonotypes Confirmed Through Cloning 
     These sequences are included in PCT/US2018/046997, filed on Aug. 17, 2018, which application is incorporated by reference in its entirety. 
     Table A 
     This table is included in PCT/US2018/06793, filed on Dec. 28, 2018, which is incorporated by reference in its entirety 
     Table A1 
     This table is included in PCT/US2018/046997, filed on Aug. 17, 2018, which is incorporated by reference in its entirety. 
     Table A2 
     This table is included in PCT/US2018/046997, filed on Aug. 17, 2018, which is incorporated by reference in its entirety.