Patent Publication Number: US-2022228157-A1

Title: Gene editing in diverse bacteria

Description:
RELATED APPLICATIONS 
     This application is a U.S. national stage application claiming the benefit of international application number PCT/US2020/034025 filed on May 21, 2020, which claims the benefit under 35 U.S.C. § 119(e) of U.S. provisional application No. 62/852,244 filed on May 23, 2019 and U.S. provisional application Ser. No. 62/951,471 filed on Dec. 20, 2019, each of which is incorporated by reference herein in its entirety. 
    
    
     GOVERNMENT LICENSE RIGHTS 
     This invention was made with government support under DE-FG02-02ER63445 awarded by the U.S. Department of Energy. The government has certain rights in the invention. 
    
    
     REFERENCE TO A SEQUENCE LISTING SUBMITTED AS A TEXT FILE VIA EFS-WEB 
     This application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jan. 21, 2022, is named H049870689US02-SUBSEQ-FL.TXT and is 1,029,796 bytes in size. 
     BACKGROUND 
     Recombineering was introduced as a term in 2001 to refer to a method for integrating linear double-stranded DNA 1  (dsDNA) or synthetic single-stranded DNA oligonucleotides (ssDNA or oligonucleotides (oligos)) 2  into the  Escherichia coli  ( E. coli ) genome by expression of the Red operon from Enterobacteria phage λ. The Red operon comprises three genes: 1) λ Exo, a 5′ to 3′ dsDNA exonuclease that loads Redβ onto resected ssDNA 3,4 ; 2) Redβ, a single-stranded annealing protein (SSAP) that anneals ssDNA to genomic DNA at the replication fork 5 ; and 3) λ Gam, a bacterial nuclease inhibitor that protects linear dsDNA from degradation 6 . Redβ, the SSAP, is required for recombineering of both ssDNA and dsDNA, whereas λ Exo and λ Gam are thought to be involved in recombineering of dsDNA. Improvements to the efficiency of ssDNA recombineering in  E. coli  have been made through the knockout of mismatch repair machinery 7  and the protection of oligos from nucleolytic degradation 8 . These improvements spurred the development of multiplexed automatable genome engineering (MAGE), a technique that for the first time envisioned the bacterial genome as a massively editable template. MAGE was applied notably to the full genomic recoding of  E. coli  MG1655 9  (removal of all amber stop codons—TAG), which has subsequently become a model chassis organism for biocontainment 10  and non-standard amino acid (NSAA) studies 11,12 . 
     SUMMARY 
     The present disclosure is based, at least in part, on unexpected data showing that pairs of single-stranded annealing proteins (SSAPs) and single-stranded binding proteins (SSBs) can be used to efficiently edit the genomes of a variety of bacterial species (not only  E. coli ) with cross-species specificity. In some embodiments, the SSAPs and SSBs are from entirely different species of bacteriophage, relative to each other, yet can still be used together for efficient recombineering. The data herein also unexpectedly demonstrate that a pair of SSB and SSAP can be used to integrate into the genome of a host cell an exogenous double-stranded nucleic acid, even in the absence of an exogenous exonuclease (e.g., a cognate exogenous exonuclease). As used herein, an exonuclease is capable of removing successive nucleotides from the end of a nucleic acid. An exonuclease may be a double-stranded exonuclease that is useful in generating a nucleic acid comprising single-stranded nucleotide overhangs. An exogenous exonuclease is an exonuclease that is introduced into a cell. A cognate exogenous exonuclease is an exonuclease that is from the same species as a SSAP, SSB, or combination thereof that is introduced into a cell. 
     Accordingly, provided herein, in some embodiments, are SSAPs that may be used together with species-matched or species-unmatched SSBs for use in editing the genome of cells (e.g., recombineering). 
     Provided herein, in some embodiments, are recombineering tools for efficient gene editing (e.g., multiplex genomic editing) in microbial cells, such as bacterial cells. The principal limitation of recombineering technology is that Redβ, does not function well in non- E. coli  bacterial species. Species-specific SSAPs have been reported for other hosts, but in comparison to  E. coli , where ssDNA recombineering efficiency has been reported at over 20% 13 , reported editing efficiency in non- E. coli  hosts is as low as 0.01% and no more than 1% 14,15 . Applications such as genomic recoding, strain engineering, or other engineering goals that require the ability to massively edit a bacterial genome are not currently possible outside of  E. coli  (i.e., without bacterial species). Furthermore, even the efficiency that has been previously reported in  E. coli  (˜20-30%) remains a limiting factor to more advanced applications that utilize a more efficient gene-editing tool. For instance, 321 edits were made to the  E. coli  MG1655 genome to recode all TAGs to TAA, but this process took about 4 years and necessitated conjugation steps to assemble the genome from partially-recoded parts. To remove or alter another native codon, thousands of mutations would need to be made. Provided herein is a more efficient editing tool to make feasible the kinds of applications that require hundreds to thousands of mutations within a shorter period of time. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIGS. 1A-1B  show matrices testing all combinations of the top seven enriched SSBs against the top four enriched SSAPs in  E. coli  ( FIG. 1A ) and  L. lactis  ( FIG. 1B ). 
         FIGS. 2A-2C  show results of editing efficiency testing for SSAPs and SSAP/single-stranded binding (SSB) pairs from experiments using  E. coli  ( FIG. 2A ),  L. lactis  ( FIG. 2B ), and  M. smegmatis  ( FIG. 2C ). 
         FIG. 3  show the results of multiplex incorporation of edits in  E. coli  populations expressing either an efficient SSAP (SEQ ID NO: 157), an efficient SSAP/SSB pair (SEQ ID NO: 157-SEQ ID NO: 384), or the widely-used Redβ (EC-Bet). 
         FIGS. 4A-4C  show the results of various experiments testing the SSAP comprising the sequence of SEQ ID NO: 24, a high-efficiency SSAP from  Pseudomonas aeruginosa  ( P. Aeruginosa ) that was identified by an early experiment with  E. coli .  FIG. 4A  shows that the SSAP SEQ ID NO: 24 displays improved annealing kinetics in vitro.  FIG. 4B  shows that the SSAP SEQ ID NO: 24 is improved over Redβ in many clinically relevant species of Gammaproteobacteria.  FIG. 4C  shows that in  P. aeruginosa , the SSAP SEQ ID NO: 24 enables rapid multi-drug resistance profiling. 
         FIG. 5  shows top individual SSAPs SEQ ID NO: 157 and SEQ ID NO: 24 expressed in  E. coli  from a high-activity promoter. The mutational profile of edits are shown, including the efficiency of making 18-nucleotide (NT) and 30-NT mismatches. 
         FIGS. 6A-6B  show that co-expression of an SSAP/SSB pair that facilitates the integration of double-stranded cassettes.  FIG. 6A  shows erythromycin colony forming units (CFUs) after expression of SSAP SEQ ID NO: 24 alone, or co-expressed with its corresponding SSB (PaSSB, SEQ ID NO: 472) or exonuclease. The SSAP/SSB pair alone is enough for cassette insertion.  FIG. 6B  shows that EcSSAP (Redβ) performs slightly better with its associated exonuclease, but the SSAP/SSB pair alone performs nearly as well. 
         FIG. 7  shows editing efficiency in  Agrobacterium tumefaciens  expressing SSAP SEQ ID NO: 143 in combination with either SSB SEQ ID NO: 310 or SSB SEQ ID NO: 368. Editing efficiency of close to 1% was measured in SSAP SEQ ID NO: 143/SSB SEQ ID NO: 310. 
         FIGS. 8A-8B  include graphs showing frequency and enrichment of members of Broad RecT Library over ten rounds of SEER enrichment.  FIG. 8A  shows the frequency of the library members.  FIG. 8B  shows the enrichment of library members. 
         FIGS. 9A-9E  show recombineering results with a broad RecT Library and CspRecT.  FIG. 9A  is a graph in which frequency is plotted against enrichment for each Broad RecT Library member after the tenth round of selection. One candidate protein, CspRecT (box), was the standout winner. In all subsequent panels, Redβ, PapRecT, and CspRecT are compared when expressed from a pORTMAGE-based construct ( FIG. 10 ) in wild-type MG1655  E. coli . Significance values are indicated for a grouped parametric t-test, where ns and ***** indicate p &gt;0.05 and p &lt;0.0001 respectively.  FIG. 9B  is a graph in which editing efficiency was measured by blue/white screening at the LacZ locus for eight different single-base mismatches (n=3).  FIG. 9C  is a graph in which editing efficiency was measured by blue/white screening at the LacZ locus for 18-base and 30-base mismatches (n=3).  FIG. 9D  shows a sample MAGE experiment that tested editing at 1, 5, 10, 15, or 20 sites at once in triplicate, was read out by NGS. The solid lines represent the average editing efficiency across all sites, while the dashed lines represent the aggregate editing efficiency.  FIG. 9E  shows a 130-oligo DIvERGE experiment using oligos that were designed to tile four different genomic loci that encode the drug targets of fluoroquinolone antibiotics and are known hotspots for CIP resistance. The oligos contained 1.5% degeneracy at each nucleotide position along their entire length. All 130 oligos were mixed and transformed together into cells (n=3). Colony forming units were measured at three different CIP concentrations after plating 1/100 th  of the final recovery volume. 
         FIGS. 10A-10B  are schematics showing vector maps.  FIG. 10A  shows pARC8-DEST, which was created to have a pBAD regulatory region, beta lactamase, a p15a origin, and a lethal ccdB gene flanked by attR sites for Gateway cloning. Introduction by the LR Gateway reaction of for instance SR001, would create the vector on the right, with an arabinose-inducible SR001 followed by a barcode.  FIG. 10B  shows two pORTMAGE vectors are provided for broad-spectrum recombineering. pORTMAGE-Ec1 was demonstrated effective in  E. coli, C. freundii , and  K. pneumoniae , while pORTMAGE-Pa1 was demonstrated effective in  P. aeruginosa.    
         FIGS. 11A-11C  depict recombineering in Gammaproteobacteria.  FIG. 11A  shows results of recombineering experiments that were run with Redβ, PapRecT, and CspRecT expressed off of the pORTMAGE311B backbone, or with a pBBR1 origin in the case of  P. aeruginosa . Editing efficiency was measured by colony counts on selective vs. non-selective plates (n=3; see methods). Vector optimization resulted in improved efficiency of PapRecT in  P. aeruginosa  (see  FIG. 13 )  FIG. 11B  is a diagram of a simple multi-drug resistance experiment in  P. aeruginosa  harboring an optimized PapRecT plasmid expression system, pORTMAGE-Pa1. In a single round of MAGE, a pool of five oligos was used to incorporate genetic modifications that would provide resistance to STR, RIF, and CIP (n=3). These populations were then selected by plating on all combinations of 1-, 2-, or 3-antibiotic agarose plates and compared with a non-selective control.  FIG. 11C  shows observed efficiencies that were calculated by comparing colony counts on selective vs. non-selective plates. Expected efficiencies for multi-locus events were calculated as the product of all relevant single-locus efficiencies. 
         FIG. 12  is a graph showing recombineering efficiency in  P. aeruginosa  was measured for PapRecT with  E. coli  codons, PapRecT with its wild-type codons, and two SSAPs that have been reported to work in  Pseudomonas putida . This was measured both with the original pORTMAGE311B RBS and an RBS optimized for  P. aeruginosa . Significance values are indicated for a parametric t-test between two groups, where ns, *, **, ***, and ***** indicate p &gt;0.05, p &lt;0.05, p &lt;0.01, p &lt;0.001, and p &lt;0.0001 respectively. 
         FIG. 13  shows editing efficiency in making a single-base mutation at the rpsL locus in  P. aeruginosa  with various plasmid variants expressing PapRecT. An unoptimized plasmid (far left) was constructed by replacing, in pORTMAGE312B (Addgene), the RSF1010 origin of replication and the kanamycin resistance gene with a pBBR1 origin of replication and a gentamicin resistance gene. The best-performing plasmid variant (third from right) was renamed pORTMAGE-Pa1 (Addgene). Constructs examining the role of MutL in single-base recombineering efficiency were made by first restoring wild-type PaMutL and then by removing it entirely (second from right, and far right respectively). 
         FIG. 14  shows results with one round of MAGE with a pool of three oligos that confer Ciprofloxacin resistance was conducted in  P. aeruginosa  with pORTMAGE-Pa1. Editing efficiency is shown after plating on three different concentrations of antibiotic. 
         FIG. 15  shows the effect of codon-usage on Redβ editing efficiency in  E. coli . The efficiency of Redβ from the Broad SSAP Library was compared with Redβ expressed off of its wild-type codons. Efficiency of making a single base pair mutation in a non-coding gene was measured by next generation sequencing (NGS). 
         FIGS. 16A-16B  include data showing the editing efficiency and growth rates of bacteria expressing a candidate from the Broad SSAP Library or Redβ.  FIG. 16A  shows the efficiency of a candidate SSAP at incorporating a single-base-pair silent mutation at a non-essential gene, ynfF. Efficiency was read out by NGS. Significance values are indicated for a parametric t-test between two groups, where ns, *, **, ***, and ***** indicate p &gt;0.05, p &lt;0.05, p &lt;0.01, p &lt;0.001, and p &lt;0.0001 respectively.  FIG. 16B  shows growth rates, which were measured by plate-reader growth assay and plotted against the maximum attained OD 600  of the culture. 
         FIGS. 17A-17H  include data showing the editing efficiency in recombinant cells comprising RecTs, SSBs, or “cognate pairs.”  FIG. 17A  shows an in-vitro model of ssDNA annealing inhibition by EcSSB or L1SSB, and ability of λ-Red β to overcome annealing inhibition by EcSSB.  FIG. 17B  shows ssDNA annealing without SSB, precoated with EcSSB, or pre-coated with L1SSB. Shaded area represents the SEM of at least 2 replicates.  FIG. 17C  shows ssDNA annealing in the presence of λ-Red β when pre-coated with EcSSB or L1SSB. Shaded area represents the SEM of at least 2 replicates.  FIG. 17D  shows a model for RecT-mediated editing in the presence of SSB. An interaction between RecT and the host SSB enables oligo annealing to the lagging strand of the replication fork. **Co-expressing an exogenous SSB that is compatible with a particular RecT variant can in some species enable efficient homologous genome editing even if host compatibility does not exist.  FIGS. 17E-17F  show calculation of editing efficiency in  L. lactis  and  E. coli  is performed by introducing antibiotic resistance mutations into the genome using synthetic oligos, and then measuring the ratio of resistant cells to total cells.  FIGS. 17G-17H  show a comparison of the efficiency of editing in  L. lactis  and  E. coli  after the expression of either RecTs, SSBs, or “cognate pairs” (see, e.g., Example 10). 
         FIGS. 18A-18F  include data showing genome editing efficiency using SSAP and chimeric SSB pairs.  FIG. 18A  shows a crystal structure of homotetrameric  E. coli  SSB bound to ssDNA (PDB-ID 1EYG)37. The amino acid sequence of the flexible C-terminal tail is diagramed in the right panel, along with the design of a 9AA C-terminal truncation to SSB.  FIG. 18B  shows a diagram of the  L. lactis  SSB C-terminal tail is diagramed, along with an example of an SSB C-terminal tail replacement. In this case, the 9 C-terminal amino acids of the  L. lactis  SSB are replaced with the corresponding residues from  E. coli  SSB. The notation “L1SSB C9:EcSSB” is used as shorthand.  FIG. 18C  shows editing efficiency in  L. lactis  of λ-Red β with a 9AA C-terminally truncated EcSSB mutant. The sequence shown for EcSSB (C10) corresponds to SEQ ID NO: 516.  FIG. 18D  shows editing efficiency in  L. lactis  of λ-Red β expressed with L1SSB, or mutants of L1SSB with C3, C7, C8, or C9 terminal residues replaced with the corresponding residues from EcSSB. The following sequences are shown from top to bottom: SEQ ID NOS: 532, 538-541 and 516.  FIGS. 18E-18F  show editing efficiency in  L. lactis  of PapRecT ( FIG. 18E ) or MspRecT ( FIG. 18F ) expressed with L1SSB, or mutants of L1SSB with the C7 or C8 terminal residues replaced with the corresponding residues from the cognate SSB. The following sequences are shown in  FIG. 18E  from top to bottom: SEQ ID NOS: 532, 542-543, and 520. The following sequences are shown in  FIG. 18F  from top to bottom: SEQ ID NOs: 532, 544-545, and 524. 
         FIGS. 19A-19F  include data evaluating RecT compatibility with distinct bacterial SSBs and chimeric SSBs.  FIGS. 19A-19B  show heat maps showing the fold improvement in editing efficiency due to SSB coexpression in ( FIG. 19A )  L. lactis  or ( FIG. 19B )  E. coli  of RecT-SSB pairs as compared to the RecT alone.  FIG. 19C  shows C-terminal sequences of SSBs as well as RecT compatibility given  FIGS. 19A and 19B .” The following sequences are shown from top to bottom: SEQ ID NOs: 516, 516, 516, 520, 524, 528, 532, and 535.  FIG. 19D  shows editing efficiency in  L. lactis  of PapRecT coexpressed with LrSSB, MsSSB, or mutants of LrSSB which had the C7 or C8 terminal residues replaced with the corresponding residues from the MsSSB. The following sequences are shown from top to bottom: SEQ ID NOS: 528, 546, 547, and 524.  FIG. 19E  shows editing efficiency in  M. smegmatis  of λ-Red β, PapRecT, MspRecT, and LrpRecT.  FIG. 19F  shows editing efficiency in  L. rhamnosus  of λ-Red β, PapRecT, MspRecT, and LrpRecT. 
         FIGS. 20A-20B  show editing efficiency in  C. crescentus  using pairs of RecT and SSB.  FIG. 20A  shows editing efficiency in  C. crescentus  of two RecT-SSB protein pairs, λ-Red β+PaSSB and PapRecT+PaSSB which had high genome editing efficiency in both  E. coli  and  L. lactis .  FIG. 20B  shows editing efficiency in  C. crescentus  of λ-Red β+PaSSB with ribosomal binding sites optimized for translation rate and using an oligo designed to evade mismatch repair. 
         FIG. 21  shows that in  L. lactis , the internal RBS sequence affected recombination efficiency using the bicistronic Redβ and EcSSB construct. RBS 2, which enabled the highest efficiency genome editing in this experiment was selected used in all other bicistronic constructs unless otherwise indicated. The sequences for RBS1-RBS4 correspond to SEQ ID NOs: 509, 507, 510 and 511, respectively. 
         FIG. 22  shows design of RBSs for use in  C. crescentus . Using the Salis et al. RBS calculator, RBSs were designed to confer a greater translation rate in order to increase RecT and SSB expression for the  Caulobacter  constructs. See, e.g., Salis et al. Nat. Biotechnol. 27, 946-50 (2009) and Borujeni et al. Nucleic Acids Res. 42, 2646-2659 (2014). The sequences shown correspond to SEQ ID NOS: 505, 506, 507, and 508 from top to bottom. 
         FIGS. 23A-23E  includes data showing genome editing efficiency of  L. lactis  comprising PapRecT, and PaSSB.  FIG. 23A  shows that in  L. lactis , optimization of nisin concentration contributed to a significant improvement in editing efficiency for the PapRecT protein and the PaSSB protein construct. 10 ng/mL nisin was much more effective than 1 ng/mL nisin and resulted in an increase in editing efficiency improvement from 0.5% to 8%. The optimal oligo amount plateaued at 50 μg of DNA, which corresponds 21.4 μM in 80 μL.  FIG. 23B  shows expression of the  L. lactis  MutL variant E33K allowed the efficient introduction of 1 bp mismatches at similar efficiency to 4 bp mismatches which evade MMR.  FIG. 23C  shows that after optimization from  FIGS. 23A-23B , PapRecT+PaSSB+LlMutLE33K enabled ˜20% editing efficiency at the Rif locus, and multiplexed editing ( FIG. 23D ).  FIG. 23E  shows that co-expression of PapRecT+PaSSB enabled the efficient introduction of a 1 kb selectable marker as dsDNA even without the addition of the cognate phage exonuclease. This also was observed for Redb with EcSSB in  L. lactis  (Data not shown). 
         FIG. 24  shows the editing efficiency of SSAP candidates in  Agrobacterium tumefaciens . Enrichment on the Y-axis is a measure of editing efficiency. 
         FIG. 25  shows the editing efficiency of SSAP candidates in  Staphylococcus aureus . Enrichment on the Y-axis is a measure of editing efficiency. 
     
    
    
     DETAILED DESCRIPTION 
     A library of 234 SSAPs was tested both individually and co-expressed with a library of 237 SSBs. These libraries were tested in  E. coli  and two model gram positive microbes:  Lactococcus lactis  (Firmicutes) and  Mycobacterium smegmatis  (Actinobacteria).  L. lactis  and  M. smegmatis  are important model systems, are distant relations of  E. coli  and of each other, and have had reports of low efficiency recombineering ( L. lactis: ˜ 0.1% 15   ; M. smegmatis: ˜ 0.01% 14 ).  L. lactis  is an industrially-relevant microbe used in dairy production of kefir, buttermilk, and cheese, and is a human commensal.  M. smegmatis  is also a human commensal, and a fast-growing model system for  M. tuberculosis . In fact, Firmicutes and Actinobacteria are two of the most highly-populated phyla of human commensals 16 . 
     Oligo recombineering efficiency was improved, as shown herein, in all three bacterial species:  E. coli  (40%),  L. lactis  (20%), and  M. smegmatis  (5%) enough to support high-throughput experimentation by recombineering without the need for selection. Top SSAPs were tested in the three chassis organisms, and in all cases supported significantly improved rates of oligo-mediated recombineering ( FIGS. 1A-1C ,  FIG. 5 ). In the SSAP/SSB library, SSAPs and SSBs were both individually enriched, and so matrices were constructed of every combination of high-performing SSAPs and high-performing SSBs ( FIGS. 2A-2B ). Through testing these in a high-throughput assay and reading out efficiency by next-generation sequencing (NGS), the highest efficiency pairs were identified. These pairs performed better than any individual SSAP ( FIGS. 1A-1C ) and allowed for double-stranded DNA cassette integration, even in the absence of an exogenous exonuclease ( FIGS. 6A-6B ). 
     Next, the multiplex incorporation of edits in  E. coli  was tested, which was demonstrative of some of the more important applications enabled by the technology provided herein. The most efficient SSAP/SSB pair in  E. coli  incorporated at close to 100% efficiency (15 edits simultaneously) after a week of MAGE cycling, as compared to Redβ, which only did so at 20% efficiency ( FIG. 3 ). 
     Finally, the efficiency of genome-editing was tested in species that had not been tested in the above-mentioned libraries. First, a highly-enriched SSAP from the  E. coli  experiments was tested in clinically relevant Gammaproteobacteria ( FIGS. 4A-4C ). It was found that the SSAP SEQ ID NO: 24 functions at high efficiency in  Pseudomonas aeruginosa , where Redβ does not work. This allows for the reconstruction of antibiotic-resistant phenotypes at high efficiency in this host, which has developed significant resistance. Next, a library of co-expressed SSAP/SSB pairs (25 most-enriched SSBs and three most-enriched SSAPs across  E. coli, L. lactis , and  M. smegmatis ) was tested in  Agrobacterium tumefaciens . This 75 member library was enriched for the most active variants over two rounds of selective MAGE, and two of the most frequent pairs were isolated. The most active of these pairs showed close to 1% editing efficiency. 
     Single-Stranded Annealing Protein (SSAP) 
     Aspects of the present disclosure provide single-stranded annealing proteins. Single-stranded annealing proteins (SSAPs) are recombinases that are capable of annealing an exogenous nucleic acid (any nucleic acid that is introduced into a cell) to a target locus in the genome of a cell. A SSAP may be from (e.g., derived from, obtained from, and/or isolated from) any SSAP superfamily, including RecT, ERF, RAD52, SAK, SAK4, and GP2.5. See, e.g., Iyer et al., BMC Genomics. 2002 Mar. 21; 3:8; Neamah et al., Nucleic Acids Res. 2017 Jun. 20; 45(11):6507-6519. In some instances, GP2.5 is from T7 phage. As a non-limiting example, SSAPs may be identified using the Pfam database. For example, RecT SSAPs may be identified under Pfam Accession No. PF03837, ERF SSAPs may be identified under Pfam Accession No. PF04404, and RAD 52 SSAPs may be identified under Pfam Accession No. PF04098. 
     As used herein, a SSAP may be from any source. For example, SSAPs may be from a virus or a bacteria. The source may be a eukaryote or a prokaryote. See, e.g., Table 1. 
     A SSAP may comprise a sequence that is least 50% (e.g., at least 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more than 99%, including all values in between) identical to a sequence selected from SEQ ID NOS: 1-234. In some instances, a SSAP comprises a sequence selected from SEQ ID NOS: 1-234. In some instances, a SSAP consists of a sequence selected from SEQ ID NOS: 1-234. 
     Single-Stranded Binding Protein (SSB) 
     The SSAPs of the present disclosure may be used with a single-stranded binding protein (SSB). SSBs bind to single-stranded nucleic acids (e.g., single-stranded nucleic acids comprising deoxyribonucleotides, ribonucleotides, or a combination thereof). The binding of a SSB to a single-stranded nucleic acid can serve numerous functions. For example, SSB binding may protect a nucleic acid from degradation. In some instances, SSB binding to a single-stranded nucleic acid reduces the secondary structure of the nucleic acid, which may increase the accessibility of the nucleic acid to other enzymes (e.g., recombinases). SSB binding can also prevent re-annealing of complementary strands during replication. As a non-limiting example, SSBs may be identified using the Pfam database under Accession Number PF00436. 
     The SSBs of the present disclosure may be from any source. For example, SSBs may be from a virus or a bacteria. The source may be a eukaryote or a prokaryote. See, e.g., Table 1. 
     A SSB may comprise a sequence that is least 50% (e.g., at least 55%, 60%, 65%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more than 99%, including all values in between) identical to a sequence selected from SEQ ID NOS: 235-472. In some instances, a SSB comprises a sequence selected from SEQ ID NOS: 235-472. In some instances, a SSB consists of a sequence selected from SEQ ID NOS: 235-472. 
     In some embodiments, a SSB is a chimeric SSB and comprises SSB sequences from two different sources. To produce a chimeric SSB, one or more amino acids in the C-terminus of the SSB may be substituted. For example, at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 26, at least 27, at least 28, at least 29, at least 30, at least 31, at least 32, at least 33, at least 34, at least 35, at least 36, at least 37, at least 38, at least 39, at least 40, at least 41, at least 42, at least 43, at least 44, at least 45, at least 46, at least 47, at least 48, at least 49, at least 50, at least 51, at least 52, at least 53, at least 54, at least 55, at least 56, at least 57, at least 58, at least 59, at least 60, at least 61, at least 62, at least 63, at least 64, at least 65, at least 66, at least 67, at least 68, at least 69, at least 70, at least 71, at least 72, at least 73, at least 74, at least 75, at least 76, at least 77, at least 78, at least 79, at least 80, at least 81, at least 82, at least 83, at least 84, at least 85, at least 86, at least 87, at least 88, at least 89, at least 90, at least 91, at least 92, at least 93, at least 94, at least 95, at least 96, at least 97, at least 98, at least 99, or at least 100 amino acids from the C-terminus of an SSB may be substituted. The C-terminus of a SSB may be substituted with at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 26, at least 27, at least 28, at least 29, at least 30, at least 31, at least 32, at least 33, at least 34, at least 35, at least 36, at least 37, at least 38, at least 39, at least 40, at least 41, at least 42, at least 43, at least 44, at least 45, at least 46, at least 47, at least 48, at least 49, at least 50, at least 51, at least 52, at least 53, at least 54, at least 55, at least 56, at least 57, at least 58, at least 59, at least 60, at least 61, at least 62, at least 63, at least 64, at least 65, at least 66, at least 67, at least 68, at least 69, at least 70, at least 71, at least 72, at least 73, at least 74, at least 75, at least 76, at least 77, at least 78, at least 79, at least 80, at least 81, at least 82, at least 83, at least 84, at least 85, at least 86, at least 87, at least 88, at least 89, at least 90, at least 91, at least 92, at least 93, at least 94, at least 95, at least 96, at least 97, at least 98, at least 99, or at least 100 amino acids from the C-terminus of another SSB. 
     In some embodiments, a chimeric SSB is used together with an SSAP that is from a bacteriophage that is capable of infecting a type of bacteria. In such instances, the chimeric SSB may comprise a C-terminal sequence from an SSB from the same source as the source of the SSAP. In some embodiments, a chimeric SSB may comprise a C-terminal SSB sequence from a bacterium that the bacteriophage the SSAP is sourced from is capable of infecting. For example, a chimeric SSB may be used in a first type of bacterial cell with an SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of, a second type of bacterial cell. The chimeric SSB may comprise a sequence encoding an SSB from the first type of bacterial cell, in which the C-terminus of this first SSB is substituted with one or more amino acids from the C-terminus of a second SSB that is from the second type of bacterial cell that the bacteriophage can infect. As a non-limiting example, the SSAP PapRecT (SEQ ID NO: 24) may be used with a chimeric SSB comprising 7, 8, 9, or 10 amino acids of the C-terminus of PaSSB (SEQ ID NO: 472). In some instances, the chimeric SSB may comprise a C-terminal sequence that includes 1, 2, 3, 4, or 5 mutations relative to a C-terminal sequence from a SSB from a bacteriophage that is capable of infecting the same type of bacteria that the SSAP is capable of infecting. 
     In some embodiments, a chimeric SSB comprises a C-terminal sequence that is at least 70%, 80%, or at least 90% identical to a sequence selected from SEQ ID NOs: 516-547. In some embodiments, a chimeric SSB comprises a sequence selected from SEQ ID NOs: 516-547. 
     Source of Proteins 
     The proteins of the present disclosure (e.g., SSAPs, SSBs, dominant negative mismatch repair enzymes, or exonucleases) may be from any source. As used herein, a source refers to any species existing in nature that naturally harbors the protein (e.g., SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof). The term “naturally” refers to an event that occurs without human intervention. For example, certain bacteriophage naturally infect bacteria, delivering a SSAP and/or SSB; thus, some bacteria naturally harbor that SSAP and/or SSB. Non-limiting examples of suitable sources of SSAPs and SSBs are provided in Table 1. 
     Many viruses, including bacteriophages, naturally encode SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof. Therefore, a source of a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof may be a virus. In some instances, the source of a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof is a bacteriophage. Bacteriophages or phages are viruses that infect bacteria and are often classified by the type of nucleic acid genome and morphology. For example, the genome of bacteriophages may be linear or circular, double-stranded or single-stranded, and may comprise deoxyribonucleotides (DNA) or ribonucleotides (RNA). After a phage inserts its genome into a bacterial host cell, the phage genome can be reproduced through the lysogenic cycle, lytic cycle, or the lysogenic cycle followed by the lytic cycle. During the lysogenic cycle, the phage genome is integrated into the host bacterium&#39;s genome. The infected bacterial cell remains intact during the lysogenic cycle and replicates the phage genome. In contrast, during the lytic cycle, the phage genome does not integrate into the host genome and the phage hijacks the host cell&#39;s machinery to replicate the phage genome, produce viral components, and assemble new viral phages. Once the new viral phages are formed, the phages lyse the host cell and are released. Viruses that infect non-bacterial host cells use similar mechanisms of replication. In some instances, the source of a SSAP, SSB, dominant negative mismatch repair enzyme, exonuclease, or a combination thereof is a virus that can infect a particular species. In some instances, the source of a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof is a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of, a particular species of bacteria. 
     A source of a SSAP or SSB may also be a cell (e.g., a prokaryotic cell or a eukaryotic cell). As used herein, a cell that is a source of a SSAP or SSB is a cell existing in nature that harbors a gene encoding the SSAP or SSB. In some instances, the SSAP or SSB is a host gene (an endogenous gene). Since viruses naturally infect cells, a source of SSAP or SSB could also be a cell existing in nature that has been naturally infected by a virus that encodes that SSAP or SSB. 
     Non-limiting examples of phages include T7 (coliphage), T3 (coliphage), K1E (K1-capsule-specific coliphage), K1F (K1-capsule-specific coliphage), K1-5 (K1- or K5-capsule-specific coliphage), SP6 ( Salmonella  phage), LUZ19 ( Pseudomonas  phage), gh-1 ( Pseudomonas  phage), and K11 ( Klebsiella  phage). 
     Non-limiting examples of a source of a SSAP, SSB, dominant negative mismatch repair enzyme, an exonuclease or a combination thereof include [ Clostridium ] methylpentosum DSM 5476,  Acetobacter orientalis  21F-2,  Acinetobacter radioresistens  SK82,  Acinetobacter  sp P8-3-8,  Acinetobacter  sp SH024, Actinobacteria bacterium OK074,  Acyrthosiphon pisum  secondary endosymbiont phage 1 (BacteriophageAPSE-1), Agathobacter rectalis (strain ATCC 33656/DSM 3377/JCM 17463/KCTC5835/VPI 0990) ( Eubacterium  rectale),  Agrobacterium rhizogenes, Ahrensia  sp R2A130 , Akkermansia  sp KLE1798 , Anaerococcus hydrogenalis  ACS-025-V-Sch4,  Avibacterium paragallinarum  JF4211,  Bacillus  phage 0305phi8-36,  Bacillus  phage SPP1 (Bacteriophage SPP1),  Bacillus  sp 1NLA3E,  Bacillus  sp 2_A_57_CT2,  Bacillus sporothermodurans, Bacillus subtilis, Bacillus subtilis  subsp  spizizenii  (strain TU-B-10),  Jeotgalibacillus marinus, Bacillus subtilis  subsp  spizizenii  (strain TU-B-10),  Jeotgalibacillus marinus, Bacillus thuringiensis  Sbt003,  Escherichia coli  VBL21-Gold(DE3)pLysS AG\′, Enterobacteria phage HK630, Enterobacteria phage lambda (Bacteriophage lambda),  Escherichia coli  TA280,  Escherichia coli  1-176-05_S3_C2,  Escherichia coli  40967,  Bacteroides caccae  ATCC 43185,  Bartonella schoenbuchensis  (strain DSM 13525/NCTC 13165/R1),  Bifidobacterium magnum, Bifidobacterium reuteri  DSM 23975,  Bordetella bronchiseptica  ( Alcaligenes bronchisepticus ),  Bordetella  phage BPP-1,  Borrelia duttonii  CR2A,  Bradyrhizobium  sp STM 3843,  Brevibacillus brevis  (strain 47/JCM 6285/NBRC 100599),  Burkholderia cenocepacia  (strain ATCC BAA-245/DSM 16553/LMG 16656/NCTC 13227/J2315/CF5610) ( Burkholderia cepacia  (strain J2315)),  Burkholderia cenocepacia  BC7,  Burkholderia  phage BcepC6B,  Burkholderia  phage BcepGomr,  Burkholderia  phage BcepNazgul,  Burkholderia  phage BcepNY3,  Campylobacter coli  80352 , Candidatus Accumulibacter  sp SK-12 , Candidatus Cloacimonas  sp SDB,  Capnocytophaga  sp oral taxon 338 str F0234,  Caulobacter vibrioides  (strain ATCC 19089/CB15) ( Caulobacter crescentus ),  Clostridium beijerinckii  (strain ATCC 51743/NCIMB 8052) (Clostridiumacetobutylicum),  Clostridium botulinum  (strain Eklund 17B/Type B),  Clostridium botulinum  C str Eklund,  Clostridium  phage phiC2 , Peptoclostridium difficile  E15,  Clostridium  phage phiMMP03 , Peptoclostridium difficile  ( Clostridium difficile ),  Clostridium  sp CAG:470,  Clostridium  sp FS41,  Clostridium sporogenes  (strain ATCC 7955/DSM 767/NBRC 16411/NCIMB 8053/NCTC 8594/PA 3679),  Collinsella stercoris  DSM 13279, Commensalibacter intestini A911, Coriobacteriales bacterium DNF00809,  Corynebacterium striatum  ATCC 6940 , Cryptobacterium curtum  (strain ATCC 700683/DSM 15641/12-3), Cyanophage PSS2 , Dermabacter  sp HFH0086 , Desulfitobacterium metallireducens  DSM 15288,  Desulfovibrio  sp FW1012B,  Dialister  sp CAG:486,  Drosophila melanogaster  (Fruit fly),  Elusimicrobium minutum  (strain Pei191),  Endozoicomonas montiporae, Endozoicomonas montiporae  CL-33, Enterobacteria phage HK022 (Bacteriophage HK022), Enterobacteria phage HK629,  Salmonella  phage HK620 (Bacteriophage HK620), Enterobacteria phage T1 (Bacteriophage T1),  Enterococcus faecalis  (strain ATCC 700802/V583),  Enterococcus faecalis  TX0027,  Enterococcus faecalis  TX0309B,  Enterococcus faecalis  TX0309A,  Enterococcus faecalis  (strain ATCC 700802/V583),  Escherichia coli, Escherichia  phage Rtp,  Escherichia  phage Tls,  Faecalibacterium  sp CAG:82,  Flavobacterium  phage 11b,  Frateuria aurantia  (strain ATCC 33424/DSM 6220/NBRC 3245/NCIMB13370) ( Acetobacter aurantius ),  Fusobacterium mortiferum  ATCC 9817,  Fusobacterium ulcerans  12-1B, gamma proteobacterium BDW918,  Gordonia soli  NBRC 108243 , Gramella forsetii  (strain KT0803),  Haemophilus influenzae, Haemophilus influenzae  NT127,  Haemophilus paraphrohaemolyticus  HK411,  Haemophilus parasuis  serovar 5 (strain SH0165),  Hafnia alvei  ATCC 51873,  Helicobacter pullorum  MIT 98-5489,  Helicobacter  sp MIT 05-5294 , Herbaspirillum  sp YR522,  Homo sapiens  (Human),  Hungatella hathewayi  DSM 13479,  Hydrogenobacter thermophilus  (strain DSM 6534/IAM 12695/TK-6),  Hydrogenovibrio marinus, Klebsiella pneumoniae  subsp  rhinoscleromatis  ATCC 13884,  Komagataeibacter oboediens, Labilithrix luteola, Lactobacillus capillatus  DSM 19910,  Lactobacillus  phage KC5a,  Lactobacillus  phage phi jlb1 , Lactobacillus  phage Lc-Nu,  Lactobacillus  phage phiadh,  Lactobacillus  phage phigle,  Lactobacillus  phage phijl1 , Lactobacillus  prophage Lj928,  Lactobacillus johnsonii  (strain CNCM 1-12250/La1/NCC 533),  Lactobacillus  prophage Lj965,  Lactobacillus johnsonii  (strain CNCM 1-12250/La1/NCC 533),  Lactobacillus reuteri, Lactobacillus rossiae  DSM 15814,  Lactobacillus ruminis  SPM0211,  Lactobacillus shenzhenensis  LY-73,  Lactococcus lactis  subsp  cremoris  (strain MG1363),  Lactococcus lactis  subsp  lactis  by diacetylactis str TIFN2,  Lactococcus lactis  subsp  lactis  (strain IL1403) (Streptococcuslactis),  Lactococcus  phage bIL309,  Lactococcus lactis  subsp  lactis  by diacetylactis str TIFN2,  Lactococcus lactis  subsp  lactis  (strain IL1403) (Streptococcuslactis),  Lactococcus  phage bIL309,  Lactococcus  phage bIL286,  Lactococcus lactis  subsp  lactis  (strain IL1403) (Streptococcuslactis),  Lactococcus  phage c2,  Lactococcus  phage LL-H ( Lactococcus delbrueckii  bacteriophage LL-H),  Lactococcus  phage phi311,  Lactococcus  phage ul36k1t1 , Lactococcus  phage ul362,  Lactococcus  phage ul361,  Lactococcus lactis, Lactococcus  phage ul36k1,  Lactococcus  phage phi311,  Lactococcus  phage ul36k1t1,  Lactococcus  phage ul362,  Lactococcus  phage ul361,  Lactococcus lactis, Lactococcus  phage ul36k1,  Lactococcus  phage SK1833,  Lactococcus  phage SK1,  Legionella pneumophila, Leifsonia xyli  subsp  xyli, Leifsonia xyli  subsp  xyli  (strain CTCB07),  Leifsonia xyli  subsp  xyli, Leifsonia xyli  subsp  xyli  (strain CTCB07),  Lentibacillus amyloliquefaciens, Leptotrichia goodfellowii  F0264,  Leuconostoc mesenteroides  subsp  mesenteroides  (strain ATCC 8293/NCDO 523),  Listeria monocytogenes, Listeria  phage A118 (Bacteriophage A118),  Listeria  phage A500 (Bacteriophage A500),  Listeria  phage B054,  Listeria monocytogenes, Listeria welshimeri  serovar 6b (strain ATCC 35897/DSM 20650/SLCC5334),  Listeria  phage PSA,  Listonella  phage phiHSIC,  Mameliella  alba,  Methylobacterium nodulans  (strain LMG 21967/CNCM 1-2342/ORS 2060),  Methyloversatilis universalis  (strain ATCC BAA-1314/JCM 13912/FAM5),  Microbacterium ginsengisoli , Microgenomates group bacterium GW2011_GWF1_44_10,  Mycobacterium brisbanense, Mycobacterium marinum  (strain ATCC BAA-535/M),  Mycobacterium  phage Che8,  Mycobacterium  phage Che8/ Mycobacterium smegmatis, Mycobacterium  phage Hamulus,  Mycobacterium  phage Dante,  Mycobacterium  phage Ardmore,  Mycobacterium  phage Llij,  Mycobacterium  phage Drago,  Mycobacterium  phage Phatniss,  Mycobacterium  phage Spartacus,  Mycobacterium  phage Boomer,  Mycobacterium  phage SiSi,  Mycobacterium  phage PMC,  Mycobacterium  phage Ovechkin,  Mycobacterium  phage Ramsey,  Mycobacterium  phage Fruitloop,  Mycobacterium  phage SG4,  Mycobacterium  phage Hamulus,  Mycobacterium  phage Dante,  Mycobacterium  phage Ardmore,  Mycobacterium  phage Llij,  Mycobacterium  phage Drago,  Mycobacterium  phage Phatniss,  Mycobacterium  phage Spartacus,  Mycobacterium  phage Boomer,  Mycobacterium  phage SiSi,  Mycobacterium  phage PMC,  Mycobacterium  phage Ovechkin,  Mycobacterium  phage Ramsey,  Mycobacterium  phage Fruitloop,  Mycobacterium  phage SG4,  Mycobacterium  phage PhatBacter,  Mycobacterium  phage Elph10,  Mycobacterium  phage 244,  Mycobacterium  phage Cjw1 , Mycobacterium  phage Phrux,  Mycobacterium  phage Lilac,  Mycobacterium  phage Phaux,  Mycobacterium  phage Quink,  Mycobacterium  phage Pumpkin,  Mycobacterium  phage Murphy,  Mycobacterium  phage PhatBacter,  Mycobacterium  phage Elph10,  Mycobacterium  phage 244,  Mycobacterium  phage Cjw1 , Mycobacterium  phage Phrux,  Mycobacterium  phage Lilac,  Mycobacterium  phage Phaux,  Mycobacterium  phage Quink,  Mycobacterium  phage Pumpkin,  Mycobacterium  phage Murphy,  Mycobacterium  phage Troll4,  Mycobacterium  phage Gumball,  Mycobacterium  phage Nova,  Mycobacterium  phage SirHarley,  Mycobacterium  phage Adjutor,  Mycobacterium  phage Butterscotch,  Mycobacterium  phage PLot,  Mycobacterium  phage PBI1,  Mycobacterium  phage Troll4,  Mycobacterium  phage Gumball,  Mycobacterium  phage Nova,  Mycobacterium  phage SirHarley,  Mycobacterium  phage Adjutor,  Mycobacterium  phage Butterscotch,  Mycobacterium  phage PLot,  Mycobacterium  phage PBI1,  Mycobacterium  phage Wildcat,  Mycobacterium smegmatis, Mycobacterium  virus Che9c,  Neisseria lactamica  Y92-1009 , Nitratireductor basaltis, Nitrolancea hollandica  Lb,  Nocardia farcinica  (strain IFM 10152),  Nocardia terpenica, Oligotropha carboxidovorans  (strain ATCC 49405/DSM 1227/KCTC 32145/OM5),  Paenibacillus alvei  DSM 29,  Paenibacillus curdlanolyticus  YK9,  Paenibacillus dendritiformis  C454,  Paenibacillus elgii  B69,  Paenibacillus lactis  154,  Paenibacillus mucilaginosus  3016,  Paenibacillus polymyxa  (strain E681),  Paenibacillus  sp FSL R7-0331,  Paenibacillus  sp P1XP2,  Paenibacillus terrae  (strain HPL-003),  Paeniclostridium sordellii  ( Clostridium sordellii ),  Parasutterella excrementihominis  CAG:233 , Parcubacteria bacterium  32_520,  Parcubacteria  group bacterium GW2011_GWA2_42_14 , Pediococcus acidilactici  DSM 20284 , Pedobacter antarcticus, Pedobacter antarcticus  4BY,  Pedobacter antarcticus, Pedobacter antarcticus  4BY,  Pelobacter propionicus  (strain DSM 2379/NBRC 103807/OttBd1),  Peptoniphilus duerdenii  ATCC BAA-1640 , Persephonella marina  (strain DSM 14350/EX-H1),  Phormidium  phage Pf-WMP3 , Photobacterium profundum  (strain SS9),  Photorhabdus luminescens  subsp  laumondii  (strain DSM 15139/CIP105565/TT01),  Pirellula  sp SH-Sr6A,  Prevotella  sp CAG:873 , Prochlorococcus  phage P-SSM2 , Prochlorococcus  phage P-SSP7,  Pseudoalteromonas lipolytica  SCSIO 04301,  Pseudomonas aeruginosa  39016,  Pseudomonas aeruginosa, Pseudomonas aeruginosa  DHS01,  Pseudomonas  phage LKA5,  Pseudomonas  phage F116,  Pseudomonas aeruginosa, Pseudomonas aeruginosa  DHS01,  Pseudomonas  phage LKA5,  Pseudomonas  phage F116,  Pseudomonas  phage vB_Pae-Kakheti25,  Pseudomonas  phage vB_PaeP_C1-14_Or,  Pseudomonas  phage vB_PaeP_p2-10_Or1 , Pseudomonas  phage PaP3,  Rhizobium loti  (strain MAFF303099) ( Mesorhizobium loti ),  Rhizobium  sp CF080 , Rhodothermus  phage RM378 , Roseateles depolymerans, Ruminococcus  sp SR1/5,  Saccharomyces cerevisiae, Saccharomyces cerevisiae  (strain ATCC 204508/S288c) (BakerVs yeast),  Saccharomyces cerevisiae  YJM1250,  Saccharomyces cerevisiae  YJM451 , Salinicoccus halodurans, Salinisphaera hydrothermalis  C41B8 , Salinispora tropica  (strain ATCC BAA-916/DSM 44818/CNB-440),  Salmonella  phage SETP3,  Salmonella  phage SS3e,  Salmonella typhimurium, Salmonella  phage ST160,  Salmonella  phage ST64T (Bacteriophage ST64T),  Serratia odorifera  DSM 4582 , Simkania negevensis  (strain ATCC VR-1471/Z),  Sodalis glossinidius  (strain  morsitans ), Source,  Sphingopyxis  sp (strain 113P3),  Spiroplasma kunkelii  CR2-3x,  Sporosarcina newyorkensis  2681,  Staphylococcus aureus  (strain Mu50/ATCC 700699),  Staphylococcus  phage 3A,  Staphylococcus  phage phi7401PVL,  Streptococcus pneumoniae, Staphylococcus aureus  (strain NCTC 8325),  Staphylococcus  phage Phil2,  Staphylococcus aureus, Staphylococcus  phage 47,  Staphylococcus  phage tp310-2,  Staphylococcus  phage 3A,  Staphylococcus  phage phi7401PVL,  Streptococcus pneumoniae, Staphylococcus aureus  (strain NCTC 8325),  Staphylococcus  phage Phil2,  Staphylococcus aureus, Staphylococcus  phage 47,  Staphylococcus  phage tp310-2,  Staphylococcus  phage 92,  Staphylococcus  phage CNPH82,  Staphylococcus  phage phi11 (Bacteriophage phi-11),  Staphylococcus  phage 80,  Staphylococcus  phage 52A,  Staphylococcus aureus  (strain NCTC 8325),  Staphylococcus  phage Pv1108,  Staphylococcus  phage SA97,  Staphylococcus  phage phi7247PVL,  Staphylococcus  phage phiETA3,  Staphylococcus aureus, Staphylococcus  phage phi5967PVL,  Stigmatella aurantiaca  (strain DW4/3-1),  Streptococcus gallolyticus  subsp  gallolyticus  TX20005,  Streptococcus infantis  SK970,  Streptococcus  phage 7201,  Streptococcus  phage A25,  Streptococcus pyogenes, Streptococcus pyogenes  serotype M2 (strain MGAS10270),  Streptococcus pyogenes  serotype M4 (strain MGAS10750),  Streptococcus pyogenes  serotype M3 (strain ATCC BAA-595/MGAS315),  Streptococcus pyogenes  GA06023,  Streptococcus pyogenes  STAB902,  Streptococcus  phage M102,  Streptococcus  phage MM1 1998,  Streptococcus pneumoniae, Streptococcus  phage MM1,  Streptococcus  phage Sfi21,  Streptococcus  phage V22,  Streptococcus pneumoniae, Streptococcus pyogenes  serotype M28 (strain MGAS6180),  Streptococcus pyogenes , Temperate phage phiNIH11,  Streptococcus pyogenes  serotype M2 (strain MGAS10270),  Streptococcus pyogenes  serotype M3 (strain ATCC BAA-595/MGAS315),  Streptococcus pyogenes  STAB902,  Streptococcus pyogenes  STAB902,  Streptococcus pyogenes, Streptococcus pyogenes  serotype M3 (strain ATCC BAA-595/MGAS315),  Streptomyces albulus, Streptomyces albus, Streptomyces albus  J1074,  Streptomyces coelicolor  (strain ATCC BAA-471/A3(2)/M145),  Streptomyces cyaneogriseus, Streptomyces cyaneogriseus  subsp  noncyanogenus, Streptomyces longwoodensis, Streptomyces noursei, Streptomyces noursei  ATCC 11455,  Streptomyces  phage VWB,  Streptomyces rimosus, Streptomyces rimosus  subsp  pseudoverticillatus, Streptomyces  sp HPH0547 , Sulfurovum  sp F506-10 , Synechococcus  phage Syn5 , Synechococcus  sp UTEX 2973,  Synechocystis  sp PCC 6803 , Thalassomonas  phage BA3 , Thermaerobacter marianensis  (strain ATCC 700841/DSM 12885/JCM10246/7p75a),  Thermus  phage phiYS40 , Thiorhodovibrio  sp 970,  Treponema socranskii  subsp  socranskii  VPI DR56BR1116=ATCC 35536,  Ureaplasma urealyticum  serovar 10 (strain ATCC 33699/Western),  Ureaplasma urealyticum  serovar 7 str ATCC 27819,  Ureaplasma parvum  serovar 3 (strain ATCC 700970),  Ureaplasma urealyticum  serovar 8 str ATCC 27618,  Ureaplasma urealyticum  serovar 4 str ATCC 27816,  Ureaplasma urealyticum  serovar 12 str ATCC 33696,  Vibrio cholerae  (strain MO10),  Vibrio cholerae, Providencia alcalifaciens  Ban1 , Vibrio cholerae  Ind4,  Vibrio cholerae  (strain MO10),  Vibrio cholerae, Providencia alcalifaciens  Ban1 , Vibrio cholerae  Ind4,  Vibrio cholerae  1587,  Vibrio natriegens  NBRC 15636=ATCC 14048=DSM 759 , Xanthobacter autotrophicus  (strain ATCC BAA-1158/Py2),  Xanthomonas  phage OP2,  Yersinia  phage YpsP-G,  Yersinia  phage phiA1122 , Yersinia  phage YpsP-G, and  Yersinia  phage phiA1122. Other sources may be used. 
     The source, in some embodiments, is a bacterial cell. The bacterial strain may be, for example,  Yersinia  spp.,  Escherichia  spp.,  Klebsiella  spp.,  Agrobacterium  spp.,  Acinetobacter  spp.,  Bordetella  spp.,  Neisseria  spp.,  Aeromonas  spp.,  Franciesella  spp.,  Corynebacterium  spp.,  Citrobacter  spp.,  Chlamydia  spp.,  Hemophilus  spp.,  Brucella  spp.,  Mycobacterium  spp.,  Legionella  spp.,  Lactococcus  spp.,  Rhodococcus  spp.,  Pseudomonas  spp.,  Helicobacter  spp.,  Salmonella  spp.,  Vibrio  spp.,  Bacillus  spp.,  Erysipelothrix  spp.,  Salmonella  spp.,  Streptomyces  spp.,  Bacteroides  spp.,  Prevotella  spp.,  Clostridium  spp.,  Bifidobacterium  spp., or  Lactobacillus  spp. In some embodiments, the bacterial cells are probiotic cells. In some instances, the source is an  Escherichia coli  ( E. coli ) cell, a  Lactococcus lactis  ( L. lactis ) cell,  Agrobacterium tumefaciens  ( A. tumefaciens ), or a  Mycobacterium smegmatis  ( M. smegmatis ) cell. 
     The source may be a gram-positive bacterial cell. Gram-positive bacterial cells stain positive in a gram stain test and often comprise a thick layer of peptidoglycan in their cell walls. Non-limiting examples of gram-positive bacterial cells include  Actinomyces  spp.,  Alicyclobacillus  spp.,  Alicyclobacillus acidoterrestris, Alicyclobacillus aeris, Alicyclobacillus contaminans, Alicyclobacillus cycloheptanicus, Alicyclobacillus dauci, Alicyclobacillus disulfidooxidans, Alicyclobacillus fastidiosus, Alicyclobacillus ferrooxydans, Alicyclobacillus fodiniaquatilis, Alicyclobacillus herbarius, Alicyclobacillus hesperidum, Alicyclobacillus kakegawensis, Alicyclobacillus macrosporangiidus, Alicyclobacillus montanus, Alicyclobacillus pomorum, Alicyclobacillus sacchari, Alicyclobacillus sendaiensis, Alicyclobacillus shizuokensis, Alicyclobacillus tengchongensis, Alicyclobacillus tolerans, Alicyclobacillus vulcanalis, Arcanobacterium  spp.,  Bacillus  spp.,  Bacillus mojavensis, Bavariicoccus  spp.,  Brachybacterium  spp.,  Brachybacterium alimentarium, Brachybacterium aquaticum, Brachybacterium conglomeratum, Brachybacterium endophyticum, Brachybacterium faecium, Brachybacterium fresconis, Brachybacterium ginsengisoli, Brachybacterium horti, Brachybacterium huguangmaarense, Brachybacterium massiliense, Brachybacterium muris, Brachybacterium nesterenkovii, Brachybacterium paraconglomeratum, Brachybacterium phenoliresistens, Brachybacterium rhamnosum, Brachybacterium sacelli, Brachybacterium saurashtrense, Brachybacterium squillarum, Brachybacterium tyrofermentans, Brachybacterium zhongshanense, Brevibacterium linens, Collinsella stercoris, Clostridioides, Clostridioides difficile  (bacteria),  Clostridium  spp.,  Clostridium acetobutylicum, Clostridium aerotolerans, Clostridium argentinense, Clostridium autoethanogenum, Clostridium baratii, Clostridium beijerinckii, Clostridium bifermentans, Clostridium botulinum, Clostridium butyricum, Clostridium cadaveris, Clostridium cellobioparum, Clostridium cellulolyticum, Clostridium cellulovorans, Clostridium chauvoei, Clostridium clostridioforme, Clostridium colicanis, Clostridium estertheticum, Clostridium fallax, Clostridium formicaceticum, Clostridium histolyticum, Clostridium innocuum, Clostridium kluyveri, Clostridium ljungdahlii, Clostridium novyi, Clostridium paradoxum, Clostridium paraputrificum, Clostridium pasteurianum, Clostridium perfringens, Clostridium phytofermentans, Clostridium piliforme, Clostridium ragsdalei, Clostridium ramosum, Clostridium saccharobutylicum, Clostridium saccharoperbutylacetonicum, Clostridium scatologenes, Clostridium septicum, Clostridium sordellii, Clostridium sporogenes, Clostridium stercorarium, Clostridium sticklandii, Clostridium straminisolvens, Clostridium tertium, Clostridium tetani, Clostridium thermosaccharolyticum, Clostridium tyrobutyricum, Clostridium uliginosum, Cnuibacter  spp.,  Coriobacteriia  spp.,  Corynebacterium, Corynebacterium amycolatum, Corynebacterium bovis, Corynebacterium diphtheriae, Corynebacterium efficiens, Corynebacterium glutamicum, Corynebacterium granulosum, Corynebacterium jeikeium, Corynebacterium macginleyi, Corynebacterium minutissimum, Corynebacterium renale, Corynebacterium ulcerans, Cutibacterium acnes, Deinococcus marmoris, Desulfitobacterium dehalogenans, Effusibacillus consociatus, Effusibacillus lacus, Effusibacillus pohliae, Enterococcus  spp.,  Enterococcus faecalis, Fervidobacterium changbaicum, Fervidobacterium gondwanense, Fervidobacterium islandicum, Fodinibacter  spp.,  Fodinibacter luteus, Gordonia soli, Georgenia ruanii, Humibacillus  spp.,  Intrasporangium  spp.,  Janibacter  spp.,  Knoellia  spp.,  Knoellia aerolata, Knoellia flava, Knoellia locipacati, Knoellia remsis, Knoellia sinensis, Knoellia subterranea, Kribbia  spp.,  Kribbia dieselivorans, Kyrpidia spormannii, Kyrpidia tusciae, Lactobacillus  spp.,  Lactobacillus acidophilus, Lactobacillus buchneri, Lactobacillus casei, Lactococcus lactis, Lactobacillus plantarum, Lactococcus lactis, Lapillicoccus  spp.,  Lapillicoccus jejuensis, Listeriaceae  spp.,  Marihabitans  spp.,  Marihabitans asiaticum, Microbispora corallina, Mycobacterium smegmatis, Nocardia  spp.,  Nocardia asteroides, Nocardia brasiliensis, Nocardia farcinica, Nocardia ignorata, Nonpathogenic organisms, Ornithinibacter  spp.,  Ornithinibacter aureus, Paeniclostridium sordellii, Pasteuria  spp.,  Phycicoccus  spp.,  Pilibacter  spp.,  Propionibacterium freudenreichii, Rathayibacter toxicus, Rhodococcus equi, Roseburia  spp.,  Rothia dentocariosa, Sarcina  spp.,  Solibacillus  spp.,  Sporosarcina  spp.,  Sporosarcina aquimarina , Sporulation in  Bacillus subtilis, Staphylococcus, Staphylococcus aureus, Staphylococcus capitis, Staphylococcus caprae, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus lugdunensis, Staphylococcus lutrae, Staphylococcus muscae, Staphylococcus nepalensis, Staphylococcus pettenkoferi, Staphylococcus pseudintermedius, Staphylococcus saprophyticus , S,  Staphylococcus schleiferi, Staphylococcus succinus, Staphylococcus warneri, Staphylococcus xylosus, Streptococcus  spp.,  Streptococcus agalactiae, Streptococcus anginosus, Streptococcus canis, Streptococcus downei, Streptococcus equi, Streptococcus bovis, Streptococcus gordonii, Streptococcus iniae, Streptococcus lactarius, Streptococcus mitis, Streptococcus mutans, Streptococcus oralis, Streptococcus parasanguinis, Streptococcus peroris, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus ratti, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus sobrinus, Streptococcus suis, Streptococcus thermophilus, Streptococcus tigurinus, Streptococcus uberis, Streptococcus vestibularis, Syntrophomonas curvata, Syntrophomonas palmitatica, Syntrophomonas sapovorans, Syntrophomonas wolfei, Syntrophomonas zehnderi, Tumebacillus algifaecis, Tumebacillus avium, Tumebacillus flagellatus, Tumebacillus ginsengisoli, Tumebacillus lipolyticus, Tumebacillus luteolus, Tumebacillus permanentifrigoris, Tumebacillus soli , and  Viridans  streptococci. 
     The source may be a gram-negative bacterial cell. Gram-negative bacterial cells do not retain the stain in a Gram staining test and often comprise a thinner peptidoglycan layer in their cell walls as compared to gram-positive bacterial cells. Non-limiting examples of gram-negative bacteria include  Vibrio aerogenes, Acidaminococcus  spp.,  Acinetobacter baumannii, Agrobacterium tumefaciens, Akkermansia glycaniphila, Akkermansia muciniphila, Anaerobiospirillum, Anaerolinea thermolimosa, Anaerolinea thermophila, Arcobacter  spp.,  Arcobacter skirrowii, Armatimonas rosea, Azotobacter salinestris, Bacteroides  spp.,  Bacteroides caccae, Bacteroides fragilis, Bacteroides thetaiotaomicron, Bacteroides ureolyticus, Bacteroidetes  spp.,  Bartonella japonica, Bartonella koehlerae, Bartonella taylorii, Bdellovibrio  spp.,  Brachyspira  spp.,  Bradyrhizobium japonicum, Budviciaceae  spp.,  Caldilinea aerophila, Cardiobacterium  spp.,  Cardiobacterium hominis , Chaperone-Usher fimbriae,  Chishuiella  spp.,  Christensenella  spp.,  Caulobacter crescentus, Chthonomonas calidirosea, Citrobacter freundii, Coxiella burnetii, Cytophaga  spp.,  Dehalogenimonas lykanthroporepellens, Desulfurobacterium atlanticum, Devosia pacifica, Devosia psychrophila, Devosia soli, Devosia subaequoris, Devosia submarina, Devosia yakushimensis, Dialister  spp.,  Dictyoglomus thermophilum, Dinoroseobacter shibae, Enterobacter  spp.,  Enterobacter cloacae, Enterobacter cowanii, Escherichia  spp.,  Escherichia coli, Escherichia fergusonii, Escherichia hermannii, Fimbriimonas ginsengisoli, Flavobacterium  spp.,  Flavobacterium akiainvivens, Fusobacterium necrophorum, Fusobacterium nucleatum, Fusobacterium polymorphum, Gluconacetobacter diazotrophicus, Haemophilus felis, Haemophilus haemolyticus, Haemophilus influenzae, Haemophilus pittmaniae, Helicobacter  spp.,  Helicobacter bizzozeronii, Helicobacter heilmannii  s.s,  Helicobacter heilmannii  sensu lato,  Helicobacter salomonis, Helicobacter suis, Helicobacter typhlonius, Kingella kingae, Klebsiella huaxiensis, Klebsiella pneumoniae, Kluyvera ascorbata, Kluyvera cryocrescens, Kozakia baliensis, Legionella  spp.,  Legionella clemsonensis, Legionella pneumophila, Leptonema illini, Leptotrichia buccalis, Levilinea saccharolytica, Luteimonas aestuarii, Luteimonas aquatica, Luteimonas composti, Luteimonas lutimaris, Luteimonas marina, Luteimonas mephitis, Luteimonas vadosa, Mariniflexile  spp.,  Megasphaera  spp.,  Meiothermus  spp.,  Meiothermus timidus, Methylobacterium fujisawaense, Morax - Axenfeld diplobacilli, Moraxella  spp.,  Moraxella bovis, Moraxella osloensis, Morganella morganii, Mycoplasma spumans, Neisseria cinerea, Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria polysaccharea, Neisseria sicca, Nitrosomonas eutropha, Nitrosomonas halophila, Nitrosomonas stercoris, Pelosinus  spp.,  Propionispora vibrioides, Proteus mirabilis, Proteus penneri, Pseudomonas  spp.,  Pseudomonas aeruginosa, Pseudomonas luteola, Pseudomonas teessidea, Pseudoxanthomonas broegbernensis, Pseudoxanthomonas japonensis, Rickettsia parkeri, Rickettsia rickettsii, Salinibacter ruber, Salmonella  spp.,  Salmonella bongori, Salmonella enterica, Samsonia  spp.,  Serratia marcescens, Shigella  spp.,  Shimwellia  spp.,  Solobacterium moorei, Sorangium cellulosum, Sphaerotilus natans, Sphingomonas gei, Sphingosinicella humi, Spirochaeta  spp.,  Sporomusa  spp.,  Stenotrophomonas  spp.,  Stenotrophomonas nitritireducens, Thermotoga neapolitana, Thorselliaceae  spp.,  Vampirococcus  spp.,  Verminephrobacter  spp.,  Vibrio  spp.,  Vibrio adaptatus, Vibrio azasii, Vibrio campbellii, Vibrio cholerae, Victivallis vadensis, Vitreoscilla  spp.,  Wolbachia  spp.,  Yersinia  spp., and  Zymophilus paucivorans.    
     Mismatch Repair Enzymes 
     Mismatch repair enzymes are involved in the detection of distortions in the secondary structure of DNA caused by incorrectly paired nucleotides and correction of these mismatches. Non-limiting examples of mismatch repair enzymes include MutS, MutH and MutL. Dominant negative mismatch repair enzymes disable mismatch repair. Non-limiting examples of dominant negative MutL include a dominant negative MutL protein that comprises an amino acid substitution corresponding to E32K in  E. coli  wild-type MutL (SEQ ID NO: 514), E33K in  L. lactis  wild-type MutL (SEQ ID NO: 512), or E36K in  P. aeruginosa  wild-type MutL (SEQ ID NO: 548). See, e.g., SEQ ID NOs: 515, 513, or 549. 
     Without being bound by a particular theory, a dominant negative mismatch repair enzyme may be from the same source as recombinant cell in which is being expressed. 
     Variants 
     The proteins described herein (e.g., SSAPs, SSBs, dominant negative mismatch repair enzymes or exonucleases) may contain one or more amino acid substitutions relative to its wild-type counterpart. Variants can be prepared according to methods for altering polypeptide sequence known to one of ordinary skill in the art such as are found in references which compile such methods, e.g. Molecular Cloning: A Laboratory Manual, J. Sambrook, et al., eds., Second Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989, or Current Protocols in Molecular Biology, F. M. Ausubel, et al., eds., John Wiley &amp; Sons, Inc., New York. Conservative substitutions of amino acids include substitutions made amongst amino acids within the following groups: (a) M, I, L, V; (b) F, Y, W; (c) K, R, H; (d) A, G; (e) S, T; (f) Q, N; and (g) E, D. 
     It should be understood that the present disclosure encompasses the use of any one or more of the SSAPs, SSBs, dominant negative mismatch repair enzymes, or exonucleases described herein as well as a SSAP, SSB, dominant negative mismatch repair enzyme, or exonuclease that share a certain degree of sequence identity with a reference protein. The term “identity” refers to a relationship between the sequences of two or more polypeptides or polynucleotides, as determined by comparing the sequences. Identity measures the percent of identical matches between the smaller of two or more sequences with gap alignments (if any) addressed by a particular mathematical model or computer program (e.g., “algorithms”). Identity of related molecules can be readily calculated by known methods. “Percent (%) identity” as it applies to amino acid or nucleic acid sequences is defined as the percentage of residues (amino acid residues or nucleic acid residues) in the candidate amino acid or nucleic acid sequence that are identical with the residues in the amino acid sequence or nucleic acid sequence of a second sequence after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent identity. Identity depends on a calculation of percent identity but may differ in value due to gaps and penalties introduced in the calculation. Variants of a particular sequence may have at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% but less than 100% sequence identity to that particular reference sequence, as determined by sequence alignment programs and parameters described herein and known to those skilled in the art. 
     The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm. Techniques for determining identity are codified in publicly available computer programs. Exemplary computer software to determine homology between two sequences include, but are not limited to, GCG program package (Devereux, J. et al. Nucleic Acids Research, 12(1): 387, 1984), the BLAST suite (Altschul, S. F. et al. Nucleic Acids Res. 25: 3389, 1997), and FASTA (Altschul, S. F. et al. J. Molec. Biol. 215: 403, 1990). Other techniques include: the Smith-Waterman algorithm (Smith, T. F. et al. J. Mol. Biol. 147: 195, 1981; the Needleman-Wunsch algorithm (Needleman, S. B. et al. J. Mol. Biol. 48: 443, 1970; and the Fast Optimal Global Sequence Alignment Algorithm (FOGSAA) (Chakraborty, A. et al. Sci Rep. 3: 1746, 2013). 
     Homologous Recombination-Mediated Genetic Engineering (Recombineering) 
     Aspects of the present disclosure provide methods of homologous recombination-mediated genetic engineering (recombineering) to produce modified cells. The modified cell may be gram-positive or gram-negative. Recombineering refers to integration of an exogenous nucleic acid into the genome of a cell using homologous recombination (genetic recombination in which nucleotide sequences are exchanged between two similar nucleic acid molecules). As used herein, an exogenous nucleic acid is any nucleic acid that is introduced into a cell. 
     The recombineering methods described herein comprise culturing a recombinant cell that comprises (1) any of the SSAPs described herein and (2) a exogenous nucleic acid comprising a sequence of interest that binds to a target locus. The exogenous nucleic acid may be single-stranded or double-stranded and may comprise ribonucleotides, deoxyribonucleotides, unnatural nucleotides, or a combination thereof. Unnatural nucleotides are nucleic acid analogues and include peptide nucleic acid (PNA), morpholine, locked nucleic acid (LNA), as well as glycol nucleic acid (GNA), threose nucleic acid (TNA). In some instances, a recombinant cell further comprises a SSB, an exonuclease or a combination thereof. For example, a recombinant cell that is capable of integrating an exogenous nucleic acid that is double-stranded may further comprise an exonuclease and SSB. The exonuclease can be used to generate 3′ overhangs of single-stranded nucleic acids for hybridization to a target locus. In some embodiments, the methods further comprise introducing a SSAP, a SSAP and a SSB, SSAP, SSB, and dominant negative mismatch repair enzyme, or a SSAP, SSB, and an exonuclease into the cell. 
     The exogenous nucleic acid comprising a sequence of interest for use in recombineering is capable of hybridizing to a target locus. The exogenous nucleic acid may be 100% complementary to the target locus or may comprise a nucleotide modification relative to the target locus. Nucleotide modifications include mutations, deletions, insertions, and unnatural nucleotides. In some instances, the exogenous nucleic acid comprises at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 55, at least 60, at least 65, at least 70, at least 75, at least 80, at least 85, at least 90, at least 95, at least 100, at least 200, at least 300, at least 400, or at least 500 nucleotide modifications relative to the target locus for integration. In some instances, the exogenous nucleic acid comprises a sequence that is at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or at least 100% complementary to the target locus for integration. 
     In some instances, the exogenous nucleic acid comprises a contiguous stretch of nucleotides that is complementary to the target locus for integration. The contiguous stretch of nucleotides may be at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 55, at least 60, at least 65, at least 70, at least 75, at least 80, at least 85, at least 90, at least 95, at least 100, at least 200, at least 300, at least 400, or at least 500 in length. 
     In some instances, the exogenous nucleic acid comprises (1) a sequence of interest that is not complementary to the target locus for integration, and (2) flanking sequences (e.g., 10 to 500 nucleotides in length) on either side of the sequence of interest that are each complementary to the target locus for integration. In some instances, the exogenous nucleic acid does not comprise flanking sequences that are each complementary to the target locus for integration. 
     In some instances, the exogenous nucleic acid does not comprise a contiguous stretch of nucleotides that is complementary to the target locus for integration, but is still capable of binding to the target locus. For example, an exogenous nucleic acid may comprise a sequence that has a mutation at every other nucleotide relative to the target locus, but still binds to the target locus. 
     One type of recombineering is multiplex automated genomic engineering (MAGE), in which more than one locus in a cell is simultaneously targeted (e.g., targeted for modification). To carry out MAGE, more than one exogenous nucleic acid is introduced into a cell. In some instances, more than one exogenous nucleic acid targeting at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 55, at least 60, at least 65, at least 70, at least 75, at least 80, at least 85, at least 90, at least 95, at least 100, at least 200, at least 300, at least 400, at least 500, at least 600, at least 700, at least 800, at least 900, at least 1,000, at least 2,000, at least 3,000, at least 4,000, at least 5,000, at least 6,000, at least 7,000, at least 8,000, at least 9,000, or at least 10,000 loci in the genome of a cell are introduced into a cell. In some instances, two or more exogenous nucleic acids target the same locus in the genome of a cell. In some instances, at least two exogenous nucleic acids target different loci in the genome of a cell. 
     As used herein, one cycle of recombineering refers to one round of inducing integration of an exogenous nucleic acid comprising a sequence of interest in one or more cells (e.g., in a population of cells). When the SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof is present on an expression vector that comprises a constitutive promoter, induction of integration of an exogenous nucleic acid may comprise introduction of one or more nucleic acids encoding a SSAP, a SSB, dominant negative mismatch repair enzyme, an exonuclease, or a combination thereof and introduction of the exogenous nucleic acid encoding a sequence of interest. When expression of SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof is under the control of an inducible promoter and the recombinant cell already comprises a nucleic acid encoding an inducible promoter operably linked to the nucleic acid encoding SSAP, SSB, dominant negative mismatch repair enzyme, exonuclease, or a combination thereof, induction of integration of an exogenous nucleic acid may comprise culturing the cell in the presence of an inducing reagent and introducing the exogenous nucleic acid to the cell. As a non-limiting example, one round of recombineering in a bacteria host cell may comprise (1) growing cells that comprise at least one exogenous nucleic acid encoding an SSAP, SSAP/SSB pair, SSAP, SSB, and dominant negative mismatch repair enzyme, or SSAP, SSB and exonuclease; (2) inducing expression of proteins if expression is under the control of an inducible promoter; (3) making the cells competent (e.g., usually placing the cells on ice and washing with water, but this step may by organism); (4) introducing one or more exogenous nucleic acids comprising a sequence of interest into the cells (e.g., by electroporation); and (5) allowing the cells to rest. For MAGE, each cycle of recombineering may further comprise introducing multiple exogenous nucleic acids targeting at least two different loci in the genome of a cell. See, e.g., Wang et al., Nature. 2009 Aug. 13; 460(7257):894-898. 
     In some instances, the methods comprise at least 1 cycle, at least 2 cycles, at least 3 cycles, at least 4 cycles, at least 5 cycles, at least 6 cycles, at least 7 cycles, at least 8 cycles, at least 9 cycles, at least 10 cycles, at least 20 cycles, at least 30 cycles, at least 40 cycles, at least 50 cycles, at least 60 cycles, at least 70 cycles, at least 80 cycles, at least 90 cycles, at least 100 cycles, at least 200 cycles, at least 300 cycles, at least 400 cycles, at least 500 cycles, at least 600 cycles, at least 700 cycles, at least 800 cycles, at least 900 cycles, or at least 1,000 cycles of recombineering. For example, the method of recombineering could be MAGE. 
     The efficiency of recombineering may be measured by any suitable method that detects integration of a sequence of interest into a target locus. As a non-limiting example, the target locus of interest may be amplified in cells following introduction and/or induction of a SSAP, SSB, dominant negative mismatch repair enzyme, an exonuclease, or a combination thereof and sequenced. Polymerase chain reaction (PCR) may be used to amplify the target locus and sequencing methods include Sanger sequencing and next generation sequencing (massively parallel sequencing) technologies. The efficiency of recombineering can be calculated as the frequency of modified alleles compared to the total number of alleles detected in a cell or in a population of cells. In instances in which the target locus to be modified encodes a protein, changes in the activity level of the protein may be used to determine editing efficiency. For example, the editing efficiency of a SSAP, a SSAP and a SSB, SSAP, SSB, and dominant negative mismatch repair enzyme, or a SSAP, SSB, and an exonuclease may be measured in a bacterial cell by using an exogenous nucleic acid encoding a modification to the LacZ locus, which encodes β-galactosidase, and the efficiency of recombineering can be measured as the level of LacZ disruption. Disruption of LacZ can be measured in a β-galactosidase assay. See also, e.g., the Materials and Methods section of the Examples below. 
     In some instances, the efficiency of recombineering is measured as the percentage of cells comprising the integrated sequence of interest. 
     The efficiency of recombineering using any of the methods described herein may be at least 0.5%, at least 1%, at least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or 100%. 
     In some instances, a recombinant cell comprising a SSAP, a SSB, dominant negative mismatch repair enzyme, an exonuclease, or a combination thereof has a recombineering efficiency that is at least 2-fold, at least 3-fold, at least 4-fold, at least 5-fold, at least 6-fold, at least 7-fold, at least 8-fold, at least 9-fold, at least 10-fold, at least 15-fold, at least 20-fold, at least 25-fold, at least 30-fold, at least 35-fold, at least 40-fold, at least 45-fold, at least 50-fold, at least 55-fold, at least 60-fold, at least 65-fold, at least 70-fold, at least 75-fold, at least 80-fold, at least 85-fold, at least 90-fold, at least 95-fold, at least 100-fold, at least 200-fold, at least 300-fold, at least 400-fold, at least 500-fold, at least 600-fold, at least 700-fold, at least 800-fold, at least 900-fold, or at least 1,000 fold greater as compared to a control cell that is of the same type as the recombinant cell but that does not comprise the SSAP, the SSB, dominant negative mismatch repair enzyme, the exonuclease, or the combination thereof. In some instances, the control cell comprises Redβ SSAP from Enterobacteria phage λ. 
     As a non-limiting example, a nucleic acid sequence encoding Redβ SSAP from Enterobacteria phage λ is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 473) 
               
               
                 ATGAGTACTGCACTTGCAACATTAGCTGGCAAGTTAGCAGAGCGTGTTGG 
               
               
                   
               
               
                 TATGGATTCAGTCGACCCTCAGGAGCTTATAACTACCTTACGTCAAACAG 
               
               
                   
               
               
                 CGTTCAAGTGTGACGCCTCTGATGCACAATTTATCGCTTTGCTTATCGTA 
               
               
                   
               
               
                 GCTAACCAGTATGGGTTGAATCCTTGGACGAAGGAGATATACGCTTTCCC 
               
               
                   
               
               
                 GGATAAGCAGAACGGTATTGTTCCTGTAGTAGGTGTCGATGGATGGAGTA 
               
               
                   
               
               
                 GAATTATCAATGAAAATCAACAGTTCGATGGCATGGACTTCGAGCAGGAT 
               
               
                   
               
               
                 AATGAATCATGTACCTGCCGTATATATAGAAAAGACCGAAATCACCCAAT 
               
               
                   
               
               
                 TTGTGTGACTGAATGGATGGATGAGTGCAGACGTGAGCCGTTCAAGACCC 
               
               
                   
               
               
                 GAGAAGGCCGTGAAATCACTGGTCCGTGGCAATCACATCCAAAGAGAATG 
               
               
                   
               
               
                 TTGCGTCACAAGGCGATGATTCAGTGCGCCCGTTTAGCTTTTGGGTTTGC 
               
               
                   
               
               
                 TGGCATTTACGACAAGGACGAAGCTGAAAGAATCGTTGAAAACACTGCAT 
               
               
                   
               
               
                 ATACCGCTGAACGACAACCGGAGCGTGACATTACGCCAGTGAATGACGAG 
               
               
                   
               
               
                 ACAATGCAGGAAATTAACACGTTGTTGATTGCTTTGGACAAAACGTGGGA 
               
               
                   
               
               
                 CGACGACTTGTTACCACTTTGTAGCCAAATTTTTCGTCGAGACATTAGAG 
               
               
                   
               
               
                 CTTCATCTGAGCTTACACAAGCTGAAGCCGTCAAGGCATTGGGGTTTTTG 
               
               
                   
               
               
                 AAACAAAAAGCTACCGAACAGAAGGTAGCGGCATAA. 
               
            
           
         
       
     
     As an example, an amino acid sequence encoding Redβ SSAP from Enterobacteria phage λ is: 
     
       
         
           
               
            
               
                 (SEQ ID NO: 474) 
               
               
                 MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKCDASDAQFIALLIV 
               
               
                   
               
               
                 ANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQD 
               
               
                   
               
               
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     The efficiency of recombineering may be measured after at least 1 cycle, at least 2 cycles, at least 3 cycles, at least 4 cycles, at least 5 cycles, at least 6 cycles, at least 7 cycles, at least 8 cycles, at least 9 cycles, at least 10 cycles, at least 20 cycles, at least 30 cycles, at least 40 cycles, at least 50 cycles, at least 60 cycles, at least 70 cycles, at least 80 cycles, at least 90 cycles, at least 100 cycles, at least 200 cycles, at least 300 cycles, at least 400 cycles, at least 500 cycles, at least 600 cycles, at least 700 cycles, at least 800 cycles, at least 900 cycles, or at least 1,000 cycles of recombineering. For example, the method of recombineering could be MAGE. 
     The efficiency of recombineering may be measured after at least 1 day, at least 2 days, at least 3 days, at least 4 days, at least 5 days, at least 6 days, at least 7 days, at least 8 days, at least 9 days, at least 10 days, at least 20 days, at least 30 days, at least 40 days, at least 50 days, at least 60 days, at least 70 days, at least 80 days, at least 90 days, at least 100 days, at least 200 days, at least 300 days, at least 400 days, at least 500 days, at least 600 days, at least 700 days, at least 800 days, at least 900 days, or at least 1,000 days of recombineering. In some instances, the method of recombineering is MAGE. The recombinant cell may be of any species and may be a prokaryotic cell or a eukaryotic cell. In some instances, the recombinant cell is a bacterial cell. The bacterial strain may be, for example,  Yersinia  spp.,  Escherichia  spp.,  Klebsiella  spp.,  Agrobacterium  spp.,  Acinetobacter  spp.,  Bordetella  spp.,  Neisseria  spp.,  Aeromonas  spp.,  Franciesella  spp.,  Corynebacterium  spp.,  Citrobacter  spp.,  Chlamydia  spp.,  Hemophilus  spp.,  Brucella  spp.,  Mycobacterium  spp.,  Legionella  spp.,  Lactococcus  spp.,  Rhodococcus  spp.,  Pseudomonas  spp.,  Helicobacter  spp.,  Salmonella  spp.,  Vibrio  spp.,  Bacillus  spp.,  Erysipelothrix  spp.,  Salmonella  spp.,  Streptomyces  spp.,  Bacteroides  spp.,  Prevotella  spp.,  Clostridium  spp.,  Bifidobacterium  spp., or  Lactobacillus  spp. In some embodiments, the bacterial cells are probiotic cells. In some instances, the recombinant cell is an  Escherichia coli  ( E. coli ) cell, a  Lactococcus lactis  ( L. lactis ) cell,  Agrobacterium tumefaciens  ( A. tumefaciens ), or a  Mycobacterium smegmatis  ( M. smegmatis ) cell. 
     A recombinant cell may comprise an SSAP, a SSB, dominant negative mismatch repair enzyme, an exonuclease, or a combination thereof that is not naturally expressed in the cell. When a recombinant cell comprises a SSAP and a SSB, the SSAP and SSB may be the same source or from a different source. The source may be the same or different species from that of the recombinant cell. In some instances, a recombinant cell may comprise a SSAP, a SSB, and an exonuclease that are all from different sources. In some instances, at least one protein selected from the SSAP, the SSB, and the exonuclease is from a source that is the same species as the recombinant cell. In some instances, the sources of all three proteins (the SSAP, the SSB, and the exonuclease) are of a different species as compared to the recombinant cell. In some instances, at least one protein selected from the SSAP, the SSB, the dominant negative mismatch repair enzyme, and the exonuclease is from a source that is the same species as the recombinant cell. 
     To make any of the proteins (e.g., SSAPs, SSBs, dominant negative mismatch repair enzyme, or exonucleases) described herein, a protein of interest can be selected and expressed in a cell using conventional methods, including recombinant technology. For example, a nucleic acid encoding a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof may be introduced into a cell. A nucleic acid, generally, is at least two nucleotides covalently linked together, and in some instances, may contain phosphodiester bonds (e.g., a phosphodiester “backbone”). A nucleic acid is considered “engineered” if it does not occur in nature. Examples of engineered nucleic acids include recombinant nucleic acids and synthetic nucleic acids. In some embodiments, an engineered nucleic acid encodes a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof. In some embodiments, a SSAP or SSB is encoded by separate nucleic acids, while in other embodiments, a single nucleic acid may encode a SSAP and a SSB (e.g., each operably linked to a different promoter, or both operably linked to the same promoter). 
     Nucleic acids encoding the SSAP, SSB, dominant negative mismatch repair enzyme, exonuclease, or a combination thereof described herein may be introduced into a cell using any known methods, including but not limited to chemical transfection, viral transduction (e.g. using lentiviral vectors, adenovirus vectors, sendaivirus, and adeno-associated viral vectors) and electroporation. For example, methods that do not require genomic integration include transfection of mRNA encoding one or more of the SSAPs, SSBs, or a combination thereof and introduction of episomal plasmids. In some embodiments, the nucleic acids (e.g., mRNA) are delivered to cells using an episomal vector (e.g., episomal plasmid). In other embodiments, nucleic acids encoding a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof may be integrated into the genome of the cell. Genomic integration methods are known, any of which may be used herein, including the use of the PIGGYBAC™ transposon system, sleeping beauty system, lentiviral system, adeno-associated virus system, and the CRISPR gene editing system. 
     In some embodiments, an engineered nucleic acid is present on an expression plasmid, which is introduced into pluripotent stem cells. In some embodiments, the expression plasmid comprises a selection marker, such as an antibiotic resistance gene (e.g., bsd, neo, hygB, pac, cat, ble, or bla) or a gene encoding a fluorescent protein (RFP, BFP, YFP, or GFP). In some embodiments, an antibiotic resistance gene encodes a puromycin resistance gene. In some embodiments, the selection marker enables selection of cells expressing a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof. 
     Any of the engineered nucleic acids described herein may be generated using conventional methods. For example, recombinant or synthetic technology may be used to generate nucleic acids encoding the SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof described herein. Conventional cloning techniques may be used to insert a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof into an expression plasmid. 
     In some embodiments, an engineered nucleic acid (optionally present on an expression plasmid) comprises a nucleotide sequence encoding a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof operably linked to a promoter (promoter sequence). In some embodiments, the promoter is an inducible promoter (e.g., comprising a tetracycline-regulated sequence). Inducible promoters enable, for example, temporal and/or spatial control of SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof expression. 
     A promoter control region of a nucleic acid sequence at which initiation and rate of transcription of the remainder of a nucleic acid sequence are controlled. A promoter may also contain sub-regions at which regulatory proteins and molecules may bind, such as RNA polymerase and transcription factors. Promoters may be constitutive, inducible, activatable, repressible, tissue-specific or any combination thereof. A promoter drives expression or drives transcription of the nucleic acid sequence that it regulates. Herein, a promoter is considered to be “operably linked” when it is in a correct functional location and orientation in relation to a nucleic acid sequence it regulates to control (“drive”) transcriptional initiation and/or expression of that sequence. 
     An inducible promoter is one that is characterized by initiating or enhancing transcriptional activity when in the presence of, influenced by or contacted by an inducing agent. An inducing agent may be endogenous or a normally exogenous condition, compound or protein that contacts an engineered nucleic acid in such a way as to be active in inducing transcriptional activity from the inducible promoter. 
     Inducible promoters for use in accordance with the present disclosure include any inducible promoter described herein or known to one of ordinary skill in the art. Examples of inducible promoters include, without limitation, chemically/biochemically-regulated and physically-regulated promoters such as saccharide-regulated promoters (e.g., arabinose-responsive promoter and xylose-responsive promoters) alcohol-regulated promoters, tetracycline-regulated promoters (e.g., anhydrotetracycline (aTc)-responsive promoters and other tetracycline responsive promoter systems, which include a tetracycline repressor protein (tetR), a tetracycline operator sequence (tetO) and a tetracycline transactivator fusion protein (tTA)), steroid-regulated promoters (e.g., promoters based on the rat glucocorticoid receptor, human estrogen receptor, moth ecdysone receptors, and promoters from the steroid/retinoid/thyroid 25 receptor superfamily), metal-regulated promoters (e.g., promoters derived from metallothionein (proteins that bind and sequester metal ions) genes from yeast, mouse and human), pathogenesis-regulated promoters (e.g., induced by salicylic acid, ethylene or benzothiadiazole (BTH)), temperature/heat-inducible promoters (e.g., heat shock promoters), and light-regulated promoters (e.g., light responsive promoters from plant cells). In some instances, the promoter (e.g., for use in  E. Coli ) is an arabinose inducible promoter. As a non-limiting example, the arabinose inducible promoter is a rhamnose-inducible promoter or pL from lamda phage. In some instances, the inducible promoter is a nisin inducible promoter. For example, a nisin inducible promoter may be used in  Lactis  spp. In some instances, the inducible promoter is a tetracycline inducible promoter. As a non-limiting example, a tetracycline inducible promoter may be used in  Mycobacterium  spp. 
     In some instances, the promoter is a p23 promoter (i.e., an auto-inducible expression system comprising the srfA promoter (P srfA ), which could be activated by the signal molecules acting in the quorum-sensing pathway for competence). See, e.g., Guan et al., Microb Cell Fact. 2016 Apr. 25; 15:66. For example, a p23 promoter may be used in  Staphylococcus aureus  or in  Bacillus subtillis  cells. 
     As used herein, a native promoter refers to a promoter that is naturally operably linked to a nucleic acid encoding a protein of interest (e.g., SSAP or SSB) and a non-native promoter refers to a promoter that is not naturally operably linked to a nucleic acid encoding the protein of interest (e.g., a SSAP or SSB). For example, as long as the promoter does not naturally drive expression of a nucleic acid encoding a protein of interest, an engineered nucleic acid comprising a non-native promoter may be a promoter that naturally exists in a cell in which the engineered nucleic acid is introduced. In some instances, the non-native promoter on the engineered nucleic acid is a promoter that does not naturally exist in the cell in which the engineered nucleic acid is introduced. As a non-limiting example, a recombinant cell may comprise an engineered nucleic acid encoding a SSAP or SSB that is from a phage. The phage genome naturally comprises a promoter that naturally drives expression of the SSAP or SSB. In this case, a non-native promoter is a promoter that is not the phage promoter that normally drives expression of the SSAP or SSB. In some instances, a recombinant cell may comprise an engineered nucleic acid encoding a SSAP or SSB that is naturally encoded by the cell and the cell comprises a promoter that is operably linked to the nucleic acid encoding the SSAP or SSB. In this case, a non-native promoter is any promoter that is not the natural promoter in the cell that normally drives expression of the SSAP or SSB. In some instances, a recombinant cell may comprise an engineered nucleic acid encoding a SSAP or SSB that is naturally encoded by another cell and the other cell comprises a promoter that is operably linked to the nucleic acid encoding the SSAP or SSB. In this case, a non-native promoter is any promoter that is not the natural promoter in the other cell that normally drives expression of the SSAP or SSB. 
     Without being bound by a particular theory, use of a non-native promoter allows for expression of a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof above basal levels in a cell. In some instances, expression from a non-native promoter increases expression of a protein of interest (e.g., SSAP or SSB) by at least 1.5-fold, at least 2-fold, at least 3-fold, at least 4-fold, at least 5-fold, at least 10-fold, at least 20-fold, at least 30-fold, at least 40-fold, at least 50-fold, at least 60-fold, at least 70-fold, at least 80-fold, at least 90-fold, at least 100-fold, at least 200-fold, at least 300-fold, at least 400-fold, at least 500 fold, at least 600-fold, at least 700-fold, at least 800-fold, at least 900-fold, or at least 1,000-fold as compared to expression from the native promoter. 
     In some embodiments, a vector encoding a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof comprises a ribosome binding site (RBS). A RBS promotes initiation of protein translation. In some embodiments, a RBS comprises a sequence that is at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identical to a sequence selected from SEQ ID NOs: 505-511. In some embodiments, a RBS comprises a sequence selected from SEQ ID NOs: 505-511. 
     In some embodiments, a nucleic acid encoding a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof is codon-optimized for expression in a particular type of bacterial cell. In some embodiments, a nucleic acid encoding a SSAP, SSB, exonuclease, dominant negative mismatch repair enzyme, or a combination thereof is not codon-optimized. 
     Additional Aspects and Embodiments of the Present Disclosure 
     In some aspects, the present disclosure provides a recombinant  Escherichia coli  ( E. coli ) cell comprising a single-stranded annealing protein (SSAP) selected from the group consisting of: a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Thalassomonas  phage, a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Herbaspirillum  sp., a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Vibrio cholerae , a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Helicobacter pullorum , and a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Methyloversatilis universalis . In some embodiments, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Thalassomonas  phage comprises the amino acid sequence of SEQ ID NO: 19, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Herbaspirillum  sp. comprises the amino acid sequence of SEQ ID NO: 201, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Vibrio cholera  comprises the amino acid sequence of SEQ ID NO: 63, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Helicobacter pullorum  comprises the amino acid sequence of SEQ ID NO: 128, and the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Methyloversatilis universalis  comprises the amino acid sequence of SEQ ID NO: 210. 
     In some embodiments, the  E. coli  cell further comprises an exogenous nucleic acid comprising a sequence of interest. In some embodiments, the nucleic acid is integrated in the genome of the  E. coli  cell. In some embodiments, the nucleic acid is a single-stranded DNA. In some embodiments, the nucleic acid is a double-stranded DNA. 
     Also provided herein are methods comprising culturing the recombinant  E. coli  cell and producing a modified  E. coli  cell comprising the sequence of interest. 
     In other aspects, the present disclosure provides a recombinant  Lactococcus lactis  ( L. lactis ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Enterococcus faecalis  and a single-stranded binding protein (SSB) selected from the group consisting of: a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptococcus  sp., a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Lactobacillus  sp., and a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Pseudomonas  sp. In some embodiments, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Enterococcus faecalis  comprises the amino acid sequence of SEQ ID NO: 5. In some embodiments, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptococcus  sp. comprises the amino acid sequence of SEQ ID NO: 366, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Lactobacillus  sp. comprises the amino acid sequence of SEQ ID NO: 381, and/or the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Pseudomonas  sp. comprises the amino acid sequence of SEQ ID NO: 395. 
     In yet other aspects, the present disclosure provides a recombinant  Lactococcus lactis  ( L. lactis ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium  sp. and a single-stranded binding protein (SSB) selected from the group consisting of: a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Escherichia coli , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Haemophilus influenzae , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptococcus  sp., and a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Lactobacillus  sp. In some embodiments, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium  sp. comprises the amino acid sequence of SEQ ID NO: 143. In some embodiments, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Escherichia coli  comprises the amino acid sequence of SEQ ID NO: 262, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Haemophilus  influenza comprises the amino acid sequence of SEQ ID NO: 325, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptococcus  sp. comprises the amino acid sequence of SEQ ID NO: 366, and/or the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Lactobacillus  sp. comprises the amino acid sequence of SEQ ID NO: 381. 
     In some embodiments, the  L. lactis  cell further comprises an exogenous nucleic acid comprising a sequence of interest. In some embodiments, the nucleic acid is integrated in the genome of the  L. lactis  cell. In some embodiments, the nucleic acid is a single-stranded DNA. In some embodiments, the nucleic acid is a double-stranded DNA. 
     Also provided herein are methods comprising culturing the recombinant  L. lactis  cell and producing a modified  L. lactis  cell comprising the sequence of interest. 
     In further aspects, the present disclosure provides a recombinant  Mycobacterium smegmatis  ( M. smegmatis ) cell comprising a single-stranded annealing protein (SSAP) selected from the group consisting of: a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium  sp., a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Microbacterium ginsengisoli , a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptomyces  sp., and a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Nocardia farcinica . In some embodiments, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium  sp. comprises the amino acid sequence of SEQ ID NO: 143, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Microbacterium ginsengisoli  comprises the amino acid sequence of SEQ ID NO: 178, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptomyces  sp. comprises the amino acid sequence of SEQ ID NO: 140, and/or the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Nocardia farcinica  comprises the amino acid sequence of SEQ ID NO: 175. 
     In some embodiments, the  M. smegmatis  cell further comprises a single-stranded binding protein (SSB). 
     In some embodiments, the  M. smegmatis  cell further comprises an exogenous nucleic acid comprising a sequence of interest. In some embodiments, the nucleic acid is integrated in the genome of the  M. smegmatis  cell. In some embodiments, the nucleic acid is a single-stranded DNA. In some embodiments, the nucleic acid is a double-stranded DNA. 
     Also provided herein are methods comprising culturing the recombinant  M. smegmatis  cell and producing a modified  M. smegmatis  cell comprising the sequence of interest. 
     In additional aspects, the present disclosure provides a recombinant  Escherichia coli  ( E. coli ) cell comprising: a single-stranded annealing protein (SSAP) selected from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of, the group consisting of a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Collinsella stercoris , a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Thalassomonas  sp., a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Vibrio cholera , and a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Helicobacter pullorum ; and a single-stranded binding protein (SSB) selected from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of, the group consisting of a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptococcus pyogenes , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Sodalis glossinidius , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium botulinum , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Salmonella  sp., a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Gordonia soli , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Paeniclostridium sordellii , and a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Staphylococcus aureus . In some embodiments, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Collinsella stercoris  comprises the amino acid sequence of SEQ ID NO: 157, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Thalassomonas  sp. comprises the amino acid sequence of SEQ ID NO: 19, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Vibrio cholera  comprises the amino acid sequence of SEQ ID NO: 63, and/or the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Helicobacter pullorum  comprises the amino acid sequence of SEQ ID NO: 128; and/or the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptococcus pyogenes  comprises the amino acid sequence of SEQ ID NO: 235, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Sodalis glossinidius  comprises the amino acid sequence of SEQ ID NO: 281, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium botulinum  comprises the amino acid sequence of SEQ ID NO: 300, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Salmonella  sp. comprises the amino acid sequence of SEQ ID NO: 308, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Gordonia soli  comprises the amino acid sequence of SEQ ID NO: 382, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Paeniclostridium sordellii  comprises the amino acid sequence of SEQ ID NO: 384, and/or the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Staphylococcus aureus  comprises the amino acid sequence of SEQ ID NO: 460. 
     In additional aspects, the present disclosure provides a recombinant  Lactococcus lactis  ( L. lactis ) cell comprising: a single-stranded annealing protein (SSAP) selected from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of, the group consisting of a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Enterococcus faecalis , a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Agrobacterium rhizogenes , a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium  sp., and a SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium botulinum ; and a single-stranded binding protein (SSB) selected from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of, the group consisting of a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Escherichia coli , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of, Enterobacteria sp., a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Haemophilus  influenza, a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptococcus , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Desulfitobacterium metallireducens , a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Lactobacillus  sp., and a SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Pseudomonas  sp. In some embodiments, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Enterococcus faecalis  comprises the amino acid sequence of SEQ ID NO: 5, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Agrobacterium rhizogenes  comprises the amino acid sequence of SEQ ID NO: 7, the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium  sp. comprises the amino acid sequence of SEQ ID NO: 143, and/or the SSAP from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Clostridium botulinum  comprises the amino acid sequence of SEQ ID NO: 37; and/or the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Escherichia coli  comprises the amino acid sequence of SEQ ID NO: 262, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of, Enterobacteria sp. comprises the amino acid sequence of SEQ ID NO: 284, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Haemophilus  influenza comprises the amino acid sequence of SEQ ID NO: 325, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Streptococcus  comprises the amino acid sequence of SEQ ID NO: 366, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Desulfitobacterium metallireducens  comprises the amino acid sequence of SEQ ID NO: 368, the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Lactobacillus  sp. comprises the amino acid sequence of SEQ ID NO: 381, and the SSB from a bacteriophage that can infect, or from a prophage that is stably integrated into the genome of,  Pseudomonas  sp. comprises the amino acid sequence of SEQ ID NO: 395. 
     Additional Embodiments 
     Additional embodiments of the present disclosure are provided in the following numbered paragraphs: 
     Paragraph 1. A recombinant bacterial cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Collinsella stercoris , wherein the SSAP is expressed from a non-native promoter. 
     Paragraph 2. The recombinant bacterial cell of paragraph 1, wherein the recombinant bacterial cell is selected from the group consisting of a recombinant  Escherichia coli  cell, a recombinant  Klebsiella pneumoniae  cell, a recombinant  Salmonella enterica  cell, and a recombinant  Citrobacter freundii  cell. 
     Paragraph 3. A recombinant  Escherichia coli  ( E. coli ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Collinsella stercoris.    
     Paragraph 4. The recombinant  E. coli  cell of paragraph 3, wherein the SSAP comprises the amino acid sequence of SEQ ID NO: 157. 
     Paragraph 5. The recombinant  E. coli  cell of paragraph 3 or 4, wherein the cell further comprises a single-stranded binding protein (SSB). 
     Paragraph 6. The recombinant  E. coli  cell of paragraph 5, wherein the SSB is selected from the group consisting of: a SSB from a bacteriophage that can infect  Clostridium botulinum , a SSB from a bacteriophage that can infect  Gordonia soli , a SSB from a bacteriophage that can infect  Paeniclostridium sordellii , and a SSB from a bacteriophage that can infect  Enterococcus faecalis.    
     Paragraph 7. The recombinant  E. coli  cell of paragraph 6, wherein the SSB from a bacteriophage that can infect  Clostridium botulinum  comprises the amino acid sequence of SEQ ID NO: 300, the SSB from a bacteriophage that can infect  Gordonia soli  comprises the amino acid sequence of SEQ ID NO: 382, the SSB from a bacteriophage that can infect  Paeniclostridium sordellii  comprises the amino acid sequence of SEQ ID NO: 384, and/or the SSB from a bacteriophage that can infect  Enterococcus faecalis  comprises the amino acid sequence of SEQ ID NO: 389. 
     Paragraph 8. The recombinant  E. coli  cell of paragraph 6, wherein the SSB is from a bacteriophage that can infect  Gordonia soli , optionally comprising the amino acid sequence of SEQ ID NO: 382. 
     Paragraph 9. The recombinant  E. coli  cell of paragraph 6, wherein the SSB is from a bacteriophage that can infect  Paeniclostridium sordellii , optionally comprising the amino acid sequence of SEQ ID NO: 384. 
     Paragraph 10. A method, comprising 
     culturing a recombinant  Escherichia coli  ( E. coli ) cell that comprises (a) a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Collinsella stercoris  and (b) a nucleic acid comprising a sequence of interest that binds to a target locus of the  E. coli  cell genome, wherein the sequence of interest comprises a nucleotide modification relative to the target locus, and 
     producing a modified  E. coli  cell comprising the sequence of interest at the target locus. 
     Paragraph 11. The method of paragraph 10, wherein the modification is a mutation, insertion, and/or deletion. 
     Paragraph 12. A recombinant  Lactococcus lactis  ( L. lactis ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Enterococcus faecalis.    
     Paragraph 13. The recombinant  L. lactis  cell of paragraph 12, wherein the SSAP comprises the amino acid sequence of SEQ ID NO: 5. 
     Paragraph 14. A recombinant  Lactococcus lactis  ( L. lactis ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Clostridium  sp. 
     Paragraph 15. The recombinant  L. lactis  cell of paragraph 14, wherein the SSAP comprises the amino acid sequence of SEQ ID NO: 143. 
     Paragraph 16. The recombinant  L. lactis  cell of any one of paragraphs 12-15, wherein the cell further comprises a single-stranded binding protein (SSB). 
     Paragraph 17. The recombinant  L. lactis  cell of paragraph 16, wherein the SSB is from a bacteriophage that can infect  Streptococcus  sp. 
     Paragraph 18. The  L. lactis  cell of paragraph 17, wherein the SSB comprises the amino acid sequence of SEQ ID NO: 366. 
     Paragraph 19. A method, comprising 
     culturing a recombinant  Lactococcus lactis  ( L. lactis ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Enterococcus faecalis  and (b) an nucleic acid comprising a sequence of interest that binds to a target locus of the  L. lactis  cell genome, wherein the sequence of interest comprises a nucleotide modification relative to the target locus, and 
     producing a modified  L. lactis  cell comprising the sequence of interest at the target locus. 
     Paragraph 20. A method, comprising 
     culturing a recombinant  Lactococcus lactis  ( L. lactis ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Clostridium  sp. and (b) an nucleic acid comprising a sequence of interest that binds to a target locus of the  L. lactis  cell genome, wherein the sequence of interest comprises a nucleotide modification relative to the target locus, and 
     producing a modified  L. lactis  cell comprising the sequence of interest at the target locus. 
     Paragraph 21. A recombinant  Mycobacterium smegmatis  ( M. smegmatis ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Legionella pneumophila.    
     Paragraph 22. The recombinant  M. smegmatis  cell of paragraph 21, wherein the SSAP comprises the amino acid sequence of SEQ ID NO: 44. 
     Paragraph 23. The recombinant  M. smegmatis  cell of paragraph 21 or 22, wherein the cell further comprises a single-stranded binding protein (SSB). 
     Paragraph 24. A method, comprising 
     culturing a recombinant  Mycobacterium smegmatis  ( M. smegmatis ) cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Legionella pneumophila  and (b) an nucleic acid comprising a sequence of interest that binds to a target locus of the  M. smegmatis  cell genome, wherein the sequence of interest comprises a nucleotide modification relative to the target locus, and 
     producing a modified  M. smegmatis  cell comprising the sequence of interest at the target locus. 
     Paragraph 25. The recombinant cell of any one of the foregoing paragraphs, wherein the cell further comprises an exogenous nucleic acid comprising a sequence of interest that binds to a target locus of the cell genome, wherein the sequence of interest comprises a nucleotide modification relative to the target locus. 
     Paragraph 26. The recombinant cell of paragraph 25, wherein the nucleic acid is a single-stranded DNA. 
     Paragraph 27. The recombinant cell of paragraph 25, wherein the nucleic acid is a double-stranded DNA. 
     Paragraph 28. The recombinant cell of any one of paragraphs 25-27, wherein the nucleic acid is integrated in the genome of the cell. 
     Paragraph 29. A method, comprising 
     culturing the recombinant cell of any one of paragraphs 25-27 and producing a modified cell comprising the sequence of interest at the target locus. 
     Paragraph 30. A method of editing the genome of  Escherichia coli  ( E. coli ) cells, comprising 
     performing multiplexed automatable genome engineering (MAGE) in  E. coli  cells that comprise (a) a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Collinsella stercoris  and (b) at least two exogenous nucleic acids, each comprising a sequence of interest that binds to at least one target locus of the  E. coli  cell genome, wherein the sequence of interest comprises a nucleotide modification relative to the target locus, and 
     producing modified  E. coli  cells comprising the sequence of interest at the target locus. 
     Paragraph 31. The method of paragraph 30, wherein the SSAP comprises the amino acid sequence of SEQ ID NO: 157. 
     Paragraph 32. The method of paragraph 30 or 31, wherein at least 50% of the cells comprise the sequence of interest, optionally following 5-10 cycles of MAGE. 
     Paragraph 33. The method of paragraph 30 or 31, wherein the  E. coli  cells further comprise a single-stranded binding protein (SSB) from a bacteriophage that can infect  Paeniclostridium sordellii.    
     Paragraph 34. The method of paragraph 33, wherein the SSB comprises the amino acid sequence of SEQ ID NO: 384. 
     Paragraph 35. The method of paragraph 33 or 34, wherein at least 50% of the cells comprise the sequence of interest, optionally following 5-10 cycles of MAGE. 
     Paragraph 36. The method of paragraph 35, wherein at least 75% of the cells comprise the sequence of interest, optionally following 5-10 cycles of MAGE. 
     Paragraph 37. A recombinant bacterial cell comprising a single-stranded annealing protein (SSAP) from a bacteriophage that can infect  Pseudomonas aeruginosa , wherein the SSAP is expressed from a non-native promoter. 
     Paragraph 38. The recombinant bacterial cell of paragraph 37, wherein the SSAP comprises the amino acid sequence of SEQ ID NO: 24. 
     Paragraph 39. The recombinant bacterial cell of paragraph 37 or 38, wherein the recombinant bacterial cell is selected from the group consisting of a recombinant  Klebsiella pneumoniae  cell, a recombinant  Salmonella enterica  cell, and a recombinant  Citrobacter freundii  cell. 
     Paragraph 40. The recombinant bacterial cell of any one of paragraphs 37-39, wherein the cell further comprises a single-stranded binding protein (SSB). 
     Paragraph 41. The recombinant bacterial cell of any one of paragraphs 37-40, wherein the cell further comprises an exogenous nucleic acid comprising a sequence of interest that binds to a target locus of the cell genome, wherein the sequence of interest comprises a nucleotide modification relative to the target locus. 
     Paragraph 42. The recombinant bacterial cell of paragraph 41, wherein the nucleic acid is a single-stranded DNA. 
     Paragraph 43. The recombinant bacterial cell of paragraph 41, wherein the nucleic acid is a double-stranded DNA. 
     Paragraph 44. The recombinant bacterial cell of any one of paragraphs 41-43, wherein the nucleic acid is integrated in the genome of the cell. 
     Paragraph 45. A method, comprising culturing the cell of any one of paragraphs 41-43 and producing a modified cell comprising the sequence of interest at the target locus. 
     Paragraph 46. A recombinant bacterial cell comprising a single-stranded annealing protein (SSAP) and/or a single-stranded binding protein (SSB) of Table 1 expressed from a non-native promoter. 
     Paragraph 47. The recombinant bacterial cell of paragraph 46, wherein the cell further comprises an exogenous nucleic acid comprising a sequence of interest that binds to a target locus of the cell genome, wherein the sequence of interest comprises a nucleotide modification relative to the target locus. 
     Paragraph 48. The recombinant bacterial cell of paragraph 47, wherein the nucleic acid is a single-stranded DNA. 
     Paragraph 49. The recombinant bacterial cell of paragraph 47, wherein the nucleic acid is a double-stranded DNA. 
     Paragraph 50. The recombinant bacterial cell of any one of paragraphs 47-49, wherein the nucleic acid is integrated in the genome of the cell. 
     Paragraph 51. A method, comprising 
     culturing the recombinant bacterial cell of any one of paragraphs 47-49 and producing a modified bacterial cell comprising the sequence of interest at the target locus. 
     Paragraph 52. A method, comprising 
     (i) introducing into a recombinant cell: (a) a single-stranded annealing protein (SSAP), (b) a single-stranded binding protein (SSB), and (c) a double-stranded nucleic acid comprising a sequence of interest that binds to a genomic target locus of the recombinant cell, wherein the sequence of interest comprises a nucleotide modification relative to the target locus, and 
     (ii) producing a modified recombinant cell comprising the sequence of interest at the target locus, wherein the modified recombinant cell does not express an exogenous exonuclease. 
     Paragraph 53. The method of paragraph 52, wherein (a) and (b) are from the same species. 
     Paragraph 54. The method of paragraph 52, wherein (a) and (b) are from different species. 
     Paragraph 55. The method of any one of paragraphs 52-54, wherein the SSAP comprises SEQ ID NO: 24. 
     Paragraph 56. The method of any one of paragraphs 52-55, wherein the SSB comprises SEQ ID NO: 472. 
     Paragraph 57. The method of paragraph 36, wherein at least 95% of the cells comprise the sequence of interest following 15 cycles of MAGE. 
     Paragraph 58. The method of paragraph 36, wherein following 15 cycles of MAGE, the percentage of cells comprising the sequence of interest is at least four-fold greater as compared to control  E. coli  cells that comprise (a) a Redβ SSAP from Enterobacteria phage λ (SEQ ID NO: 474) and (b) the at least two exogenous nucleic acids, each comprising the sequence of interest that binds to a different target locus of the control  E. coli  cell genome, wherein the sequence of interest comprises the nucleotide modification relative to the target locus. 
     EXAMPLES 
     Example 1 
     A library of 234 SSAPs were tested both individually and co-expressed with a library of 237 SSBs (Table 1, below). In the SSAP/SSB library, SSAPs and SSBs were both individually enriched, so matrices to test all combinations of the top seven enriched SSBs against the top four enriched SSAPs in  E. coli  and  L. lactis  were constructed ( FIGS. 1A-1B ). The experiment was carried out in a 96-well electroporation set-up. The relative efficiencies are clearly discernable. 
     Top-performing SSAPs and SSAP/SSB pairs from experiments in  E. coli, L. lactis , and  M. smegmatis  are shown in  FIG. 2A ,  FIG. 2B  and  FIG. 2C , respectively. Bars in red are the proteins that had previously been reported in the literature. The proteins listed were found after ten rounds of selection for protein variants that enabled the introduction of an oligonucleotide that conferred a genomic edit that provided antibiotic resistance. Unbiased editing efficiency was tested in each case by introducing a non-coding base change at a non-essential gene and measuring the frequency of incorporation via next generation sequencing. 
     Example 2 
       E. coli  populations expressing either an efficient SSAP (SEQ ID NO: 157), an efficient SSAP/SSB pair (SEQ ID NO: 157/SEQ ID NO: 384), or the widely-used Redβ were taken through fifteen cycles of MAGE and transformed each cycle with a 10 μM pool comprising 15 unique oligos. Editing efficiency at each targeted locus was measured by NGS and averaged ( FIG. 3 ). 
     The results showed the high-efficiency of SEQ ID NO: 157/SEQ ID NO: 384 for gene editing. This pair incorporated at close to 100% efficiency 15 separate mutations in one week, as compared to Redβ, which in the same time incorporated only at ˜20% efficiency. 
     Example 3 
     The efficiency of genome-editing was tested in species that had not been tested in the previously-mentioned libraries. SSAP SEQ ID NO: 24, a high-efficiency SSAP from  Pseudomonas aeruginoas  ( P. aeruginosa ) was identified by an early experiment in  E. coli . This protein displayed improved annealing kinetics in vitro ( FIG. 4A ). It showed improved efficiency over Redβ in many clinically relevant species of Gammaproteobacteria ( FIG. 4B ). In  P. aeruginosa , it enabled rapid multi-drug resistance profiling ( FIG. 4C ). Four oligonucleotides were incorporated in one day and two cycles of MAGE, conferring resistance to three antibiotics at once. 
     SSAP SEQ ID NO: 24 has not previously been described, and it displayed high activity in many clinically relevant Gammaproteobacteria.  Pseudomonas aeruginosa, Klebsiella pneumoniae , and  Salmonella enterica  were all chosen for their clinical relevance. Human infections of these bugs can acquire multi-drug resistance, becoming super-bugs. A gene-editing tool such as MAGE facilitates study of resistance trajectories. 
     Example 4 
     Top individual SSAPs (SEQ ID NO: 157 and SEQ ID NO: 24, using Redβ as a control) were expressed in  E. coli  from a lambda pL promoter. The mutational profile of edits are shown in  FIG. 5 , including the efficiency of introducing 18-nucleotide (NT) and 30-NT mismatches. Efficiency was measured by disruption of LacZ, plating on X-gal, and counting the number of blue vs. white colonies. In contrast to  FIG. 1A , a high over-performance by the SSAP (SEQ ID NO: 157) alone was observed when it was driven off of a more efficient promoter. It performed at about double the efficiency of Redβ or SSAP SEQ ID NO: 24. 
     Co-expression of an SSAP/SSB pair facilitated the integration of double-stranded cassettes. Erythromycin colony forming units (CFUs) were tested after expression of SSAP SEQ ID NO: 24 alone, or co-expressed with its corresponding SSB or exonuclease ( FIG. 6A ). The SSAP/SSB pair alone was enough for cassette insertion. EcSSAP (Redβ), performed slightly better with its associated exonuclease, but the SSAP/SSB pair alone performed nearly as well ( FIG. 6B ). These results show co-expression of an SSAP and SSB together can not only facilitate oligo-mediated cloning, but can improve the efficiency of double-stranded cassette integration. 
     The PaSSB used in this example is encoded by the following nucleic acid sequence. 
     
       
         
           
               
            
               
                 (SEQ ID NO: 475) 
               
               
                 ATGGCCCGTGGAGTGAACAAAGTAATTCTTGTCGGTAATGTGGGTGGGGA 
               
               
                   
               
               
                 TCCAGAGACGCGATACATGCCAAACGGGAACGCCGTGACAAATATCACCT 
               
               
                   
               
               
                 TAGCCACGAGCGAATCTTGGAAGGACAAACAAACAGGTCAGCAACAAGAA 
               
               
                   
               
               
                 CGAACCGAATGGCATAGAGTTGTATTTTTTGGCCGACTTGCTGAGATCGC 
               
               
                   
               
               
                 GGGTGAGTACCTTAGAAAGGGTTCTCAGGTTTATGTCGAGGGCTCATTAA 
               
               
                   
               
               
                 GAACACGTAAGTGGCAGGGGCAGGACGGGCAAGACCGATATACAACTGAA 
               
               
                   
               
               
                 ATAGTAGTGGACATAAACGGCAACATGCAACTTCTTGGTGGCAGACCGAG 
               
               
                   
               
               
                 TGGGGACGATTCACAGAGAGCTCCAAGAGAACCTATGCAGCGACCACAGC 
               
               
                   
               
               
                 AGGCTCCTCAACAGCAGTCTCGTCCGGCCCCTCAGCAGCAACCGGCTCCG 
               
               
                   
               
               
                 CAACCTGCACAAGATTACGATAGTTTTGATGATGATATTCCATTCTAA. 
               
            
           
         
       
     
     Example 5 
     A library of the most broadly-acting three (3) SSAPs and twenty five (25) SSBs was cloned into an  Agrobacterium tumefaciens  ( A. tumefaciens ) vector (75-member library). The library was selected for efficient genome editing, and oligo-recombineering. Efficiency was measured from the two most frequent members of the library after two rounds of selection. Editing efficiency of close to 1% was measured in SSAP SEQ ID NO: 143/SSB SEQ ID NO: 310. The results demonstrate that a relatively small library of broadly acting SSAP/SSB pairs can produce active variants in a novel bacterial species.  A. tumefaciens  is quite distantly related to  E. coli, L. lactis , and  M. smegmatis  ( FIG. 7 ). 
     Example 6 
     By investigating the distribution of efficient recombineering-functions across the seven principal families of phage-derived SSAPs, the initial SEER screen suggested the RecT family (Pfam family: PF03837) as the most abundant source of recombineering proteins for  E. coli . Therefore, it was determined whether by screening additional RecT variants, again exploiting the increased throughput of SEER compared to previous efforts, one might discover recombineering proteins further improved over Redβ and PapRecT. To this aim a second library was constructed, identifying a maximally diverse group of 109 RecT variants, 106 of which were synthesized successfully, which was called Broad RecT Library (see Methods for more details). Next, as previously described, 10 rounds of SEER selection was performed on Broad RecT Library ( FIGS. 8A-8B ), and upon plotting frequency against enrichment after the final selection, a clear winner emerged ( FIG. 9A ). This protein, which was referred to as CspRecT (UniParc ID: UPI0001837D7F), originates from a phage of the Gram-positive bacterium  Collinsella stercoris.    
     To maximize the phylogenetic reach and applicability of these new tools, CspRecT was characterized, alongside Redβ and PapRecT, subcloned into the pORTMAGE plasmid system ( FIGS. 10A-10B , Addgene accession: #120418). This plasmid contains a broad-host RSF1010 origin of replication, establishes tight regulation of protein expression with an m-toluic-acid inducible expression system, and disables MMR by transient overexpression of a dominant-negative mutant of  E. coli  MutL (MutL E32K) (Nyerges et al., Proc. Natl. Acad. Sci. U.S.A. 113, 2502-2507 (2016)), which makes it possible to establish high-efficiency editing without modification of the host genome. Measured with a standard lacZ recombineering assay, wild-type  E. coli  MG1655 expressing CspRecT exhibited editing efficiency of 35-51% for various single-base mismatches, averaging 43% or more than double the efficiency of cells expressing Redβ or PapRecT off of the same plasmid system ( FIG. 9B ). This pORTMAGE plasmid expressing CspRecT was referred to as pORTMAGE-Ec1 (Addgene). Without being bound by a particular theory, the efficiency of CspRecT single-locus genome editing reported here is the first to significantly exceed 25%, the theoretical maximum for a single incorporation event (Pines et al., ACS Synth. Biol. 4, 1176-1185 (2015)), implying that editing occurs either at multiple forks or over successive rounds of genome replication. 
     CspRecT was then tested at a variety of more complex genome editing tasks. For longer strings of consecutive mismatches, which are lower efficiency events, CspRecT was again about twice as efficient as Redβ. Wild type  E. coli  MG1655 expressing CspRecT displayed 6% or 3% efficiency (vs. 3% or 1% for Redβ) for the insertion of oligos conferring 18-bp or 30-bp consecutive mismatches into the lacZ locus respectively ( FIG. 9C ). To further investigate the performance of CspRecT at complex, highly multiplexed genome editing tasks, a set of 20 oligos spaced evenly around the  E. coli  genome was designed, each of which incorporates a single-nucleotide synonymous mutation at a non-essential gene. Next, while expressing Redβ, PapRecT, and CspRecT separately from the corresponding pORTMAGE plasmid, a single cycle of genome editing was performed with equimolar pools of 1, 5, 10, 15, and 20 oligos and assayed editing efficiency at each locus by PCR amplification coupled to targeted next generation sequencing (NGS). NGS analysis revealed a general trend: as the number of parallel edits grew, the degree of overperformance by CspRecT also grew ( FIG. 9D ). For instance, when making 19 simultaneous edits (one oligo from the pool of 20 could not be read out due to inconsistencies in allelic amplification), CspRecT averaged 5.1% editing efficiency at all loci, whereas Redβ and PapRecT averaged only 0.40% and 0.43%. Importantly, despite keeping total oligo concentration fixed across all pools, aggregate editing efficiency increased as more oligos were present in each pool. For instance, when using CspRecT with a 19-oligo pool, aggregate editing efficiency was nearly 100%, implying that across the total recovered population of  E. coli  there averaged one edit per cell. 
     Finally, based on the increased integration efficiency with CspRecT in multiplexed genome editing tasks, its performance was also tested in a directed evolution with random genomic mutations (DIvERGE) experiment (Nyerges et al., Proc. Natl. Acad. Sci. U.S.A. 115, E5726-E5735 (2018)). DIvERGE uses large libraries of soft-randomized oligos that have a low basal error rate at each nucleotide position along their entire sequence to incorporate mutational diversity into a targeted genomic locus. To compare the performance of Redβ, PapRecT, and CspRecT, one round of DIvERGE mutagenesis was performed by simultaneously delivering 130 partially overlapping DIvERGE oligos designed to randomize all four protein subunits of the drug targets of ciprofloxacin (gyrA, gyrB, parC, and parE) in  E. coli  MG1655. Following library generation, cells were subjected to 250, 500, and 1,000 ng/mL ciprofloxacin (CIP) on LB-agar plates. Variant libraries that were generated by expressing CspRecT produced more than ten times as many colonies at low CIP concentrations (i.e., 250 ng/mL) as Redβ and PapRecT, while at 1,000 ng/mL CIP, which requires the simultaneous acquisition of at least two mutations (usually at gyrA and parC) to confer a resistant phenotype, only the use of CspRecT produced resistant variants ( FIG. 9E ). Because gyrA and parC mutations are usually necessary to confer high-level CIP resistance, sequence analysis of gyrA and parC from 11 randomly selected CIP-resistant colonies, many different mutations were found, in combinations of up to three (data not shown). In sum, in both MAGE and DIvERGE experiments, which require multiplex editing, CspRecT provided more than an order of magnitude improvement to editing efficiency over Redβ, the current state-of-the-art recombineering tool. 
     Example 7 
     SSAPs frequently show host tropism (Sun et al., Appl. Microbiol. Biotechnol. 99, 5151-5162 (2015); Yin et al., iScience 14, 1-14 (2019); Ricaurte et al., Microb. Biotechnol. 11, 176-188 (2018)), but there are also indications that within bacterial clades certain SSAPs may function broadly (van Pijkeren et al., Nucleic Acids Res. 40, e76 (2012); Nyerges et al., Proc. Natl. Acad. Sci. U.S.A. 113, 2502-2507 (2016); van Kessel et al., Nat. Rev. Microbiol. 6, 851-857 (2008)). Therefore, the functionality of PapRecT and CspRecT in selected Gammaproteobacteria was investigated and their efficiency was compared to that of Redβ. Efforts were focused on two enterobacterial species:  Citrobacter freundii  ATCC 8090 and  Klebsiella pneumoniae  ATCC 10031, along with the more distantly related  Pseudomonas aeruginosa  PAO1. Pathogenic isolates of  K. pneumoniae  and  P. aeruginosa  are among the most concerning clinical threats due to widespread multidrug resistance (Tommasi et al., Nat. Rev. Drug Discov. 14, 529-542 (2015)). In these species, oligo-recombineering based multiplexed genome editing (i.e., MAGE and DIvERGE) holds the promise of enabling rapid analysis of genotype-to-phenotype relationships and predicting future mechanisms of antimicrobial resistance (Nyerges et al., Proc. Natl. Acad. Sci. U.S.A. 115, E5726-E5735 (2018); Szili et al., bioRxiv 495630 (2018) doi:10.1101/495630.  C. freundii , by contrast, is an intriguing biomanufacturing host in which the optimization of metabolic pathways has remained challenging (Yang et al., Biochem. Eng. J. 57, 55-62 (2011); Jiang et al., Appl. Microbiol. Biotechnol. 94, 1521-1532 (2012)). 
     To test the activity of PapRecT and CspRecT in these three organisms, the broad-host-range pORTMAGE system (Nyerges et al., Proc. Natl. Acad. Sci. U.S.A. 113, 2502-2507 (2016)) was built on as described above. For experiments in  E. coli  PapRecT or CspRecT was subcloned in place of Redβ into pORTMAGE311B (Szili et al., Antimicrob. Agents Chemother. AAC.00207-19 (2019) doi:10.1128/AAC.00207-19) ( FIGS. 10A-10B ; Addgene accession: #120418), which transiently disrupts MMR with EcMutL_E32K, and whose RSF1010 origin of replication and m-toluic-acid-based expression system allows the plasmid to be deployed over a broad range of bacterial hosts (Honda et al., Proc. Natl. Acad. Sci. U.S.A. 88, 179-183 (1991); Gawin et al., Microb. Biotechnol. 10, 702 (2017)). These same pORTMAGE-based constructs were used for testing in  C. freundii  and  K. pneumoniae . In  P. aeruginosa  the plasmid architecture remained constant, except that the origin of replication and antibiotic resistance were replaced, instead using the broad-host-range pBBR1 origin, which was shown to replicate in  P. aeruginosa  (Szpirer et al., J. Bacteriol. 183, 2101-2110 (2001)), and a gentamicin resistance marker ( FIGS. 10A-10B ). Next these constructs were tested (See methods for details), and in all three species, PapRecT and CspRecT displayed high editing efficiencies ( FIG. 11A ). In  C. freundii  and  K. pneumoniae , just as in  E. coli , CspRecT was found to be the optimal choice of protein, whereas in  P. aeruginosa  PapRecT performed the best. PapRecT was further compared to two recently reported  Pseudomonas putida  SSAPs (Rec2 and Ssr) (Ricaurte et al., Microb. Biotechnol. 11, 176-188 (2018); Aparicio et al., Microb. Biotechnol. 11, 176-188 (2018)), and found that PapRecT, isolated from a large  E. coli  screen performed equal to or better than proteins found in smaller screens run through  P. putida  ( FIG. 12 ). It was found, however, that the efficiency of the plasmid construct was lower in  P. aeruginosa  than in the enterobacterial species that pORTMAGE was optimized for. Therefore, to increase editing efficiency in  P. aeruginosa , i.) ribosomal binding sites (RBS) for PapRecT and EcMutL were optimized, ii.) EcMutL_E32K was replaced with its equivalent homologous mutant from  P. aeruginosa  (PaMutL_E36K), iii.) the native  P. aeruginosa  coding sequence for PapRecT was incorporated instead of the  E. coli  codon-optimized version ( FIG. 13 ). Together these changes significantly improved the editing efficiency of the best plasmid construct featuring PapRecT in  P. aeruginosa , which was called pORTMAGE-Pa1 (Addgene), to −15%. 
     Virulent strains of  P. aeruginosa  are a frequent cause of acute infections in healthy individuals, as well as chronic infections in high-risk patients, such as those suffering from cystic fibrosis (Marvig et al., Nat. Genet. 47, 57-64 (2015)). The rate of antibiotic resistance in this species is growing, with strains adapting quickly to all clinically applied antibiotics (AbdulWahab et al., Lung India Off. Organ Indian Chest Soc. 34, 527-531 (2017); Tacconelli et al., Lancet Infect. Dis. 18, 318-327 (2018)). The development of multidrug resistance in  P. aeruginosa  requires the successive acquisition of multiple mutations, but due to the lack of efficient tools for multiplex genome engineering in  P. aeruginosa  (Agnello et al., J. Microbiol. Methods 98, 23-25 (2014); Chen et al., iScience 6, 222-231 (2018)), investigation of these evolutionary trajectories has remained cumbersome. Therefore, and to demonstrate the utility of pORTMAGE-Pa1-based MAGE in  P. aeruginosa , a panel of genomic mutations that individually confer resistance to STR, RIF, and fluoroquinolones (i.e., CIP) were simultaneously incorporated (Cabot et al., Antimicrob. Agents Chemother. 60, 1767-1778 (2016); Jatsenko et al., Mutat. Res. 683, 106-114 (2010)). Importantly, the corresponding genes are also clinical antibiotic targets in  P. aeruginosa  (PEW ChariTable Trust. Antibiotics Currently in Global Clinical Development; pew.org/1YkUFkT). Following a single cycle of MAGE delivering 5 mutation-carrying oligos, a single-day experiment with pORTMAGE-Pa1, all possible combinations of five resistant mutations were able to be isolated, with more than 10 5  cells from a 1 ml overnight recovery attaining simultaneous resistance to STR, RIF, and CIP ( FIG. 11B ). Interestingly, because rpsL and rpoB, the resistant loci for STR and RIF respectively, are located only ˜5 kb apart from each other on the  P. aeruginosa  genome, these two mutations co-segregated much more often than would be expected by independent inheritance, confirming that co-selection functions similarly in  P. aeruginosa  to  E. coli  ( FIG. 11C ) (Wang et al., Nat. Methods 9, 591-593 (2012)). By genotyping and characterizing resistant colonies, the Minimum Inhibitory Concentration (MIC) of CIP for various resistant genotypes could be determined (Table 2,  FIG. 14 ). GyrA_T83I displays strong positive epistasis with ParC_S87L, and so clonal populations with mutations to parC but not gyrA were not pulled out of the antibiotic selection (Marcusson et al., PLoS Pathog. 5, e1000541 (2009)). The allure of this method is that the entire workflow took only three days to complete, in contrast with other genome engineering methods (i.e., CRISPR/Cas9 or base-editor-based strategies) that are either less effective, have biased mutational spectra, and/or would require tedious plasmid cloning and cell manipulation steps (Agnello et al., J. Microbiol. Methods 98, 23-25 (2014); Chen et al., iScience 6, 222-231 (2018)). 
     Example 8 
     The performance of Redβ expressed off of its wild-type codons against the codon-optimized version that was included in Broad SSAP Library. This revealed significantly decreased efficiency for the codon-optimized version of Redβ ( FIG. 15 ), which indicates that codon choice is an important consideration for library design. 
     Example 9 
     A set of five Broad SSAP library members that exhibited both high frequency and enrichment were chosen for further analysis. Their recombineering efficiency was tested against Redβ expressed off of its wild-type codons on the same plasmid system used for the SEER selections. To ensure an accurate measurement the efficiency of each SSAP was queried by NGS after performing a silent, non-coding genetic mutation at a non-essential gene, ynfF (silent mismatch MAGE oligo). Broad SSAP Library member: SR016, noted above as PapRecT (UniParc ID: UPI0001E9E6CB), demonstrated the highest efficiency of recombineering among the five Broad SSAP candidates, i.e. 31%±2% ( FIG. 16A ). The impact of these SSAPs on growth rate is shown in  FIG. 16B . 
     See also, e.g., Wannier et al., bioRxiv 2020.01.14.906594 (doi.org/10.1101/2020.01.14.906594), which is incorporated by reference in its entirety. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 SSAP and SSB_Library 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                   
                   
                 SEQ 
               
               
                   
                   
                   
                   
                   
                 ID 
               
               
                 Protein 
                 Type 
                 Family 
                 Source 
                 Amino Acid Sequence 
                 NO: 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 N001 
                 SSAP 
                 recT 
                 
                   Lactobacillus reuteri 
                 
                 MTNQVAQQQKPTKLTDLVLDRVKQMQDTQDLSPKNYNASN 
                 1 
               
               
                   
                   
                   
                   
                 ALNAAFLELQKVQDRNHRPALEVCSHDSIVKSLLDMTLQGLSP 
                   
               
               
                   
                   
                   
                   
                 AKDQCYFIVYGNELQMQRSYFGTVAAVKRLDGVKKVRAEVV 
                   
               
               
                   
                   
                   
                   
                 HEKDDFEIGANEDMELVVKRFVPKFENQDNQIIGAFAMIKTDE 
                   
               
               
                   
                   
                   
                   
                 GTDFTVMTKKEIDQSWAQTRQKNNKVQQNESQEMAKRTVEN 
                   
               
               
                   
                   
                   
                   
                 RAAKMFINTSDDSDLLTGAINDTTSNEYDDERRDVTPVEDEKQ 
                   
               
               
                   
                   
                   
                   
                 STDKLLEGFQKSQEAKAKGVSNDGNSNEGKETSEEVADGQTE 
                   
               
               
                   
                   
                   
                   
                 LFSEGTIKPADEADS 
                   
               
               
                   
               
               
                 N002 
                 SSAP 
                 recT 
                 
                   Lactobacillus reuteri 
                 
                 MTNQVANTQQITVKQFVNMNSTKKRFEDVLGKRAPQFMSSLIS 
                 2 
               
               
                   
                   
                   
                   
                 IVNSDQNLQRVEAASVINSALVAAALDLPINPNFGYMYIVPYN 
                   
               
               
                   
                   
                   
                   
                 GQAQPQMGYKGYIQLAQRSGQYRKITVAELYEDEFISWDPLM 
                   
               
               
                   
                   
                   
                   
                 EELKYEAHREKERDEKEQPVGYFGHFELLNGFQKTVYWTRQQ 
                   
               
               
                   
                   
                   
                   
                 VDNRRKRFSQAGGKNSDKPKGVWAKNYNAMALKTVIKDLLT 
                   
               
               
                   
                   
                   
                   
                 KWGPMTVDMQTAYGADEEEYNENPRDVTPVQDTASAQSEEG 
                   
               
               
                   
                   
                   
                   
                 YQTQDILNSFDQAEKEKASKEEAKPAKKATKTAKKTVKKGDE 
                   
               
               
                   
                   
                   
                   
                 VNNANESQEELFPDGTITPHAK 
                   
               
               
                   
               
               
                 N003 
                 SSAP 
                 recT 
                 
                   Escherichia coli 
                 
                 MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLA 
                 3 
               
               
                   
                   
                   
                   
                 AALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQL 
                   
               
               
                   
                   
                   
                   
                 GLEPGSALGHAYLLPFGNKNEKSGKKNVQLIIGYRGMIDLARR 
                   
               
               
                   
                   
                   
                   
                 SGQIASLSARVVREGDEFSFEFGLDEKLIHRPGENEDAPVTHVY 
                   
               
               
                   
                   
                   
                   
                 AVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEE 
                   
               
               
                   
                   
                   
                   
                 MAKKTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGE 
                   
               
               
                   
                   
                   
                   
                 YSVIDNSEE 
                   
               
               
                   
               
               
                 N004 
                 SSAP 
                 recT 
                   Enterococcus faecalis  (strain 
                 MSNDLTQITQRSLDEQVIGNLNRLQEQGLEMPPGYSPQNALKS 
                 4 
               
               
                   
                   
                   
                 ATCC 700802/V583) 
                 AFFELTNNSGGNLLQLAANNPETKTSISNALLDMVIQGLSPAKK 
                   
               
               
                   
                   
                   
                   
                 QCYFIKYGNKVQLMRSVFGTMAVLDRVTGGADITPVVVREGD 
                   
               
               
                   
                   
                   
                   
                 VFEIAMDGPDLVVAKHETAFENLDNDIKAAYVVIKLANGKEVT 
                   
               
               
                   
                   
                   
                   
                 TVMTKKQIDKSWSKAKTKNVQNDFPEEMAKRTVINRAAKYLI 
                   
               
               
                   
                   
                   
                   
                 NTSNDNDLFVQAAKDTLENEFERKDVTPEREEQAAVLEEKLFS 
                   
               
               
                   
                   
                   
                   
                 NNIKAVDQENENERITRVADVPEQPDIEQAKPIEKDNLTKVAD 
                   
               
               
                   
                   
                   
                   
                 QILEEPVQETLDVMAGYETNQKESEADVSTIEEDDYPF 
                   
               
               
                   
               
               
                 N005 
                 SSAP 
                 recT 
                   Enterococcus faecalis  TX0027 
                 MGNELIVSVQNRIQEMQHGEGLRLPTGYSVGNALNSAYLILSD 
                 5 
               
               
                   
                   
                   
                   
                 NSKGKSLLEKCHPTSVSKALLNMAIQGLSPAKNQCYFVPYGDQ 
                   
               
               
                   
                   
                   
                   
                 CTLMRSYFGSVSILERLSNVKKVHAEVIFEGDEFEIGSEDGRTV 
                   
               
               
                   
                   
                   
                   
                 VTNFKPSFLNRDNPIIGAFAWVEQTDGIKVYTIMTKKEIDKSWS 
                   
               
               
                   
                   
                   
                   
                 KAKTKNVQNDYPQEMAKRTVLSRAAKMFINSSSDNDLLVKAI 
                   
               
               
                   
                   
                   
                   
                 NETTEDEYDNNQQRKDITPNPPNIEKLEKSIFNQDENKKIAQDM 
                   
               
               
                   
                   
                   
                   
                 IDSIDLNQADKDLQEELNIEFPDPSKNYLATGEVNGDVENEDGP 
                   
               
               
                   
                   
                   
                   
                 YPF 
                   
               
               
                   
               
               
                 N006 
                 SSAP 
                 recT 
                 
                   Bacillus subtilis 
                 
                 MAKNDDIRNQLANKVNSVQKEDKPKTLADYLNDMKPELQKALP 
                 6 
               
               
                   
                   
                   
                   
                 EHITPERITRIALTTIRSNPGLQQCSPASLLGAVMQSAQLGLEP 
                   
               
               
                   
                   
                   
                   
                 GLVGHCYFVPFNKKIKGQNGAPDQWVKEVQFIIGYKGMIDLA 
                   
               
               
                   
                   
                   
                   
                 RRSGHIESIYAHAVYEKDEFDYELGLHPKLVHKPSTGHRGEMT 
                   
               
               
                   
                   
                   
                   
                 HVYAVAHFKDGGYQFDVFSKQDIENVRLRSKSKDNGPWQTDY 
                   
               
               
                   
                   
                   
                   
                 EEMAKKTVIRRMWKYLPISIEIQKQVAQDETVRKDITSEAQSVY 
                   
               
               
                   
                   
                   
                   
                 DDNVLDLGGNQFLSEPQQLNEKPSAQDADPFDGKPVDISEDDL 
                   
               
               
                   
                   
                   
                   
                 PFD 
                   
               
               
                   
               
               
                 N007 
                 SSAP 
                 recT 
                 
                   Agrobacterium rhizogenes 
                 
                 MTQTAERQPRSVLVDMSMRYGMEPAAFEATVRATCMKPDKN 
                 7 
               
               
                   
                   
                   
                   
                 GKVPSREEFAAFLLVAKEYNLNPLLKEIYAYPAKGGGIVPIVSV 
                   
               
               
                   
                   
                   
                   
                 DGWVNLINSQAALDGLEFAIEHTDVGALVSITCRIYRKDRSRPI 
                   
               
               
                   
                   
                   
                   
                 EVTEYLSECIRNTEPWAMKHRMLRHKALIQCARYAFGEAGIYD 
                   
               
               
                   
                   
                   
                   
                 EDEGEKIAGMKDVTPPMPPAPPKPPAPPKPDETIADADGVVIEH 
                   
               
               
                   
                   
                   
                   
                 EEPTVEDVAAANEDVIDDTTYFENLEEAMAVVSDAASLEEVW 
                   
               
               
                   
                   
                   
                   
                 SDFDPLSRFDGKPQGEVNQGIALAIRKRAEKRIGGAA 
                   
               
               
                   
               
               
                 N008 
                 SSAP 
                 recT 
                   Mycobacterium  virus Che9c 
                 MAENAVTKQDSPKAPETISQVLQVLVPQLARAVPKGMDPDRIA 
                 8 
               
               
                   
                   
                   
                   
                 RIVQTEIRKSRNAKAAGIAKQSLDDCTQESFAGALLTSAALGLE 
                   
               
               
                   
                   
                   
                   
                 PGVNGECYLVPYRDTRRGVVECQLIIGYQGIVKLFWQHPRASRI 
                   
               
               
                   
                   
                   
                   
                 DAQWVGANDEFHYTMGLNPTLKHVKAKGDRGNPVYFYAIVE 
                   
               
               
                   
                   
                   
                   
                 VTGAEPLWDVFTADEIRELRRGKVGSSGDIKDPQRWMERKTA 
                   
               
               
                   
                   
                   
                   
                 LKQVLKLAPKTTRLDAAIRADDRPGTDLSQSQALALPSTVKPT 
                   
               
               
                   
                   
                   
                   
                 ADYIDGEIAEPHEVDTPPKSSRAQRAQRATAPAPDVQMANPDQ 
                   
               
               
                   
                   
                   
                   
                 LKRLGEIQKAEKYNDADWFKFLADSAGVKATRAADLTFDEAK 
                   
               
               
                   
                   
                   
                   
                 AVIDMFDGPNA 
                   
               
               
                   
               
               
                 SR001 
                 SSAP 
                 sak 
                   Lactococcus  phage 
                 MSVFEQLNAINVNSKVEQKKTGKTSLSYLSWSWAWAEFKKVC 
                 9 
               
               
                   
                   
                   
                 SK1833,  Lactococcus  phage SK1 
                 PTATYEIKKFDDGKGKLVPYLVDNSLGIMVFTSVTVDDITHEM 
                   
               
               
                   
                   
                   
                   
                 WLPVMDGANKAMKFDSYTYKTKFGEKTVEPASMFDVNKTIM 
                   
               
               
                   
                   
                   
                   
                 RCLVKNLAMFGLGLYIVSGEDLPDLTEEQKELEAEKQRLREIQP 
                   
               
               
                   
                   
                   
                   
                 ALNRAEELGYPNMELLKTKTKKEIFDIMKIWLAQQETEKGE 
                   
               
               
                   
               
               
                 SR002 
                 SSAP 
                 recT 
                   Hafnia alvei  ATCC 51873 
                 MSNTMMLAPQTFDQAMQFANAIAASQFAPSSYRGKPNDVLIA 
                 10 
               
               
                   
                   
                   
                   
                 MQMGAELGFQPMQSVQGIAVINGRPSVWGDALRALILSAPDL 
                   
               
               
                   
                   
                   
                   
                 AEFEESYDEATQTAHCKISRREQTGSIATENSSESVTDAQTAGL 
                   
               
               
                   
                   
                   
                   
                 WGKNGPWKQYPKRMQQWRALGFCARDSYADRLKGIQLAEEVQD 
                   
               
               
                   
                   
                   
                   
                 YEPIEKVVHTPSQDQDSAIENKITEEQSNRINEILISVDSTFDD 
                   
               
               
                   
                   
                   
                   
                 LKKACKSMTGRDIDNQSELTSTEAAKLISSLERKLASKTGGEKD 
                   
               
               
                   
                   
                   
                   
                 AA 
                   
               
               
                   
               
               
                 SR003 
                 SSAP 
                 recT 
                   Corynebacterium striatum  ATCC 
                 MSKEIARTQDHLNEMMNWSRAMSQGNLMPRQYQGNPANLM 
                 11 
               
               
                   
                   
                   
                 6940 
                 FAAEYADALGISRIHVLTSIAVINGRPSPSADLMSAMVRQHGHK 
                   
               
               
                   
                   
                   
                   
                 LRVAGDDTYAEAVLIRSDDPDFEYTARWDESKARKAGLWGN 
                   
               
               
                   
                   
                   
                   
                 KGPWSLYPGAMLRARAISEVVRMGASDVMAGGIYTPEEVGAV 
                   
               
               
                   
                   
                   
                   
                 VDESGHVVEQPAQHKATRQQAQPQDNSAQARLANMLDATPA 
                   
               
               
                   
                   
                   
                   
                 NTDDPEVWADRIAEAGSEQELMELYATASTNPEWESAIKAMFT 
                   
               
               
                   
                   
                   
                   
                 ARKQQILLDAQYADEAEALDAELVEDTTEESAA 
                   
               
               
                   
               
               
                 SR004 
                 SSAP 
                 recT 
                   Lactococcus lactis  subsp  lactis   
                 MSNQITKTQQTLKSPEVKKKFEEVEGKKTEGFVASLLSVVGNS 
                 12 
               
               
                   
                   
                   
                 bv  diacetylactis  str TIFN2, 
                 NLKNADANSVMTAAMKAATLDLPIEPSLGFAYVIPYGREAQFQ 
                   
               
               
                   
                   
                   
                   Lactococcus lactis  subsp  lactis   
                 IGYKGFIQLALRSGQLTGLNCGIVYESQFVSYDPLFEELELDFSQ 
                   
               
               
                   
                   
                   
                 (strain IL1403) 
                 QASGDAVGYFASMKLANGFKKVTYWSKEQVLAHKKKFVKSA 
                   
               
               
                   
                   
                   
                 ( Streptococcus lactis ), 
                 NGPWRDHFDAMAQKTVLKAMLTKVAPASIESKMIQTAITEDD 
                   
               
               
                   
                   
                   
                   Lactococcus  phage bIL309 
                 SERFENAKDVTPDEPVISIDESMTSEVSQNEPATESQEQLPEDEV 
                   
               
               
                   
                   
                   
                   
                 EELFPIGKS 
                   
               
               
                   
               
               
                 SR005 
                 SSAP 
                 recA 
                   Mycobacterium  phage Che8 
                 MSLSFKPATREASYARIALSGPSGSGKTYTALALGTALADKVA 
                 13 
               
               
                   
                   
                   
                   
                 VIDTERGSASKYVGLNGWQFDTVQPDSFSPLSLVELLGLAAGG 
                   
               
               
                   
                   
                   
                   
                 EYGCVIVDSLSHYWMGVDGMLEQADRHAVRGNTFAGWKEV 
                   
               
               
                   
                   
                   
                   
                 RPDERRMIDALVSYPGHVIVTMRSKTEYVIEENERGKKTPRKV 
                   
               
               
                   
                   
                   
                   
                 GMKPEQRDGIEYEFDVVGDLDHDNTLTVVKSRIHTLAKAVVP 
                   
               
               
                   
                   
                   
                   
                 MPGEEFAHQIRDWLSDGARVPTVAEYRKQALAAETREELKAL 
                   
               
               
                   
                   
                   
                   
                 YDEVSGHKLTVAPTVDRDGNSTVLGDLITDLAREMKRAEA 
                   
               
               
                   
               
               
                 SR006 
                 SSAP 
                 gp2.5 
                   Xanthomonas  phage OP2 
                 MSIADHVAIFLAGSLDAPKAGKAKPDRPEFWGLFAFPPSAGAD 
                 14 
               
               
                   
                   
                   
                   
                 LTAACQAAAGGSLAGMRLAPKLHSRLEPDKQFAGIPQDWLIVR 
                   
               
               
                   
                   
                   
                   
                 MGTGPDFPPALFLLDGSSVQALPINGAKIRTDLFAGQKVRVNA 
                   
               
               
                   
                   
                   
                   
                 HGFAYPPKNGGPAGVSFSLDGVMAVGGGERRSSSSEGGEPSES 
                   
               
               
                   
                   
                   
                   
                 VFAKYRAEVAAAPAASTAPATTGNPFQQSAAGTDNPFG 
                   
               
               
                   
               
               
                 SR007 
                 SSAP 
                 gp2.5 
                   Burkholderia  phage BcepNY3 
                 MSTIDKLAGYEAILTHHSIITPQINKLKPTKPAEFYALIALPAAA 
                 15 
               
               
                   
                   
                   
                   
                 QADLWAILCERATSAFGHANNEEHGIKTNATSKKPIAGVPGDA 
                   
               
               
                   
                   
                   
                   
                 LVVRAASQYAPEIYDADGTLLNPQNPAHLQTIKAKFFAGTRVR 
                   
               
               
                   
                   
                   
                   
                 TILTPFHWTFQGRNGVSFNLAGIMLVPSEAQRLAIGGVDTASAF 
                   
               
               
                   
                   
                   
                   
                 KKFAQPGTGGVPATAGAPTDAAAAFAAGGNPDAAGGTLPANP 
                   
               
               
                   
                   
                   
                   
                 NPFAQQTGSAAGAGGNPFL 
                   
               
               
                   
               
               
                 SR008 
                 SSAP 
                 gp2.5 
                   Pseudomonas  phage 
                 MSKKVSQRFTFPVAKLIFPYIVTPDTEYGEVYQVTICIPTKEQAD 
                 16 
               
               
                   
                   
                   
                 vB_PaeP_C1-14_Or,  Pseudomonas   
                 ELVAKMESKDARLKGTIKYTERDGEFLFKVKQKKHVDWMQD 
                   
               
               
                   
                   
                   
                 phage vB_PaeP_p2- 
                 GERKSAVMKPIVLTSDNKPYDGPNPWGGSTGEVGILIETQKGA 
                   
               
               
                   
                   
                   
                 10_Or1,  Pseudomonas  phage PaP3 
                 RGKGTITALRLRGVRLHEIVSGGDGEDDPLFGGGFTEEEDKPED 
                   
               
               
                   
                   
                   
                   
                 VFGEDFDDEDAPI 
                   
               
               
                   
               
               
                 SR009 
                 SSAP 
                 recA 
                 
                   Paenibacillus dendritiformis 
                 
                 MSDRRAALEMALRQIEKQFGKGSIMKLGESNHMQVEIVPSGSL 
                 17 
               
               
                   
                   
                   
                 C454 
                 ALDIALGIGGLPRGRIIEVYGPESSGKTTVALHAIAEAQKVGGQ 
                   
               
               
                   
                   
                   
                   
                 AAFIDAEHALDPTYASKLGVNIDELLLSQPDTGEQALEIAEALV 
                   
               
               
                   
                   
                   
                   
                 RSGAVDIIVIDSVAALVPKAEIEGEMGDSHVGLQARLMSQALR 
                   
               
               
                   
                   
                   
                   
                 KLSGAISKSKTIAIFINQLREKVGVMFGNPETTPGGRALKFYSTI 
                   
               
               
                   
                   
                   
                   
                 RLDVRRVETIKQGNDMIGNRTRIKVVKNKVAPPFKQADIDIMY 
                   
               
               
                   
                   
                   
                   
                 GEGISREGSIVDIGTEMDIIQKSGAWYSYEGERLGQGRENAKQF 
                   
               
               
                   
                   
                   
                   
                 LKENGELALTIENKVREASNLSTVVRNHSEHDAEEAEDALELE 
                   
               
               
                   
                   
                   
                   
                 LES 
                   
               
               
                   
               
               
                 SR010 
                 SSAP 
                 recT 
                   Neisseria lactamica  Y92-1009 
                 MSIAQNQAVALAKQFNIQGDPQELVQTLKATAFKGNATDAQF 
                 18 
               
               
                   
                   
                   
                   
                 NALMIVSTQYGLNPFTKEIYAFPDKNNGITPVVGVDGWARIINS 
                   
               
               
                   
                   
                   
                   
                 HPQFDGMEFAADAESCTCKIHRKDRNHPTIVTEYLEECRRNTQ 
                   
               
               
                   
                   
                   
                   
                 PWNSHPRRMLRHKAMIQAARLAFGFGGIYDEDEAQRIQTTETP 
                   
               
               
                   
                   
                   
                   
                 ETPKEVKADPELDSLLAEGEAAANKGIEEYKKWFSEIGATGRL 
                   
               
               
                   
                   
                   
                   
                 KLGSENHERFKQIAANTIEAETAETLKPTPTEEQFAALVEAVST 
                   
               
               
                   
                   
                   
                   
                 GVKEVAEVLEAYALTDEQAAEINAL 
                   
               
               
                   
               
               
                 SR011 
                 SSAP 
                 ERF 
                   Thalassomonas  phage BA3 
                 MSKSELVTQDQSSEPVMAEPHMRLIEIAVEKGADITQLEKLMD 
                 19 
               
               
                   
                   
                   
                   
                 LQERYEANQAKKDFNEAMSKFQSLLPTIEKSGVVDYTTNKGRT 
                   
               
               
                   
                   
                   
                   
                 YYDYAKLEDIAKAIRPALKETGLSYRFSQSQNQGWITVTCIVTH 
                   
               
               
                   
                   
                   
                   
                 ASGHSEVSELTSQPDVSGGKDPLKAIASAISYLRRYTLTGSLGIV 
                   
               
               
                   
                   
                   
                   
                 VGGEDDDGGNHQEANDETDCYSDEEFKKNFPNWEKAILAGKK 
                   
               
               
                   
                   
                   
                   
                 TPEQIIKAGNAQGITFSQQQLETIEKVGNV 
                   
               
               
                   
               
               
                 SR012 
                 SSAP 
                 recT 
                   Commensalibacter intestini  A911 
                 MVPKTFTPADIVVAVQLGTSIGLSVAQSLHNIAIINGKPSIYGDM 
                 20 
               
               
                   
                   
                   
                   
                 MLALCRASPECEYVKEEMEGNKKEEWVAICTVKRKGNPEVIS 
                   
               
               
                   
                   
                   
                   
                 KFSWQDAVDAKLTGKPGPWLSYPKRMLQMRARGFALRDAFP 
                   
               
               
                   
                   
                   
                   
                 DLLNGLISQEEAQDYPTQTIEPPPVQLQSKPVAEQEVIQEMPSIE 
                   
               
               
                   
                   
                   
                   
                 PEKSELIKRYDWLVGQLTDIESREYLEKLTSQTKIINLRNELTEK 
                   
               
               
                   
                   
                   
                   
                 EPKLAAVITDLIEQALASFEEQGELANAV 
                   
               
               
                   
               
               
                 SR013 
                 SSAP 
                 sak4 
                   Mycobacterium  phage 
                 MVQSKIIKVADDEDYVNLLVYGDSGVGKTVFCGSDDKVLFVA 
                 21 
               
               
                   
                   
                   
                 Troll4,  Mycobacterium  phage 
                 PEDNSDGLLSAKLAGTTADKWPIRDWGDLVEAYNYLDELDEIP 
                   
               
               
                   
                   
                   
                 Gumball,  Mycobacterium  phage 
                 YNWIVVDSLTEMQIMAMRDILDRAVEENPSRDPDIPQIQDWQK 
                   
               
               
                   
                   
                   
                 Nova,  Mycobacterium  phage 
                 YYEMVKRMIKCFNALPVNVLYTALSRQTEDEEGTEYLLPDLQ 
                   
               
               
                   
                   
                   
                 SirHarley,  Mycobacterium  phage 
                 GKKDNYAKQVVSWMTSFGCMQIKRVRVKTDDDIAKKVKEVR 
                   
               
               
                   
                   
                   
                 Adjutor,  Mycobacterium  phage 
                 RITWKDTGLVTGKDRTNALTPYTDIRDVTDPEDDGLTLKDIRL 
                   
               
               
                   
                   
                   
                 Butterscotch,  Mycobacterium   
                 RIERKKSGSAKSATTKRPARKTASARTQKESA 
                   
               
               
                   
                   
                   
                 phage PLot,  Mycobacterium   
                   
                   
               
               
                   
                   
                   
                 phage PBI1 
                   
                   
               
               
                   
               
               
                 SR014 
                 SSAP 
                 recT 
                   Ureaplasma urealyticum  serovar 
                 MVKDDKLVPSIRLLTPNELSEQWANPNSEINQITRAVLTIQGIDL 
                 22 
               
               
                   
                   
                   
                 12 str ATCC 33696 
                 KAIDLNQAAQIIYFCQANNLNPLNKEVYLIQMGNRLAPIVGIHT 
                   
               
               
                   
                   
                   
                   
                 MTERAYMSGRLVGITQSYNDTNKSAKTTLTIRIPNLKELGVIEA 
                   
               
               
                   
                   
                   
                   
                 EVFLSEYSTNKNLWLTKPITMLKKVSLAHALRLSGLLAFKGDT 
                   
               
               
                   
                   
                   
                   
                 PYIYEEMQQGEAVPNKKMFTPPVAEVIEPAVENIKKVDFNEF 
                   
               
               
                   
               
               
                 SR015 
                 SSAP 
                 ERF 
                   Paenibacillus alvei  DSM 29 
                 MGLKRSESITNLAAALVKFQKEVVSPKNNANNPYFSSKYAPLH 
                 23 
               
               
                   
                   
                   
                   
                 EVINVIREPLAKYGLSYIQSTSTDEQNVTVTTLLMHESGEFVESE 
                   
               
               
                   
                   
                   
                   
                 PLSLPGLQVLKGGGKDFTPQGIGSAITYGRRYSLTAILGIASEDD 
                   
               
               
                   
                   
                   
                   
                 TDGNEGTPDPRNNPSKGKNQGTGAQTGTGGVKKTIEAKYKLL 
                   
               
               
                   
                   
                   
                   
                 HDNSLDGLTDFIKEHGANAEQVLTQMLMDR 
                   
               
               
                   
               
               
                 SR016 
                 SSAP 
                 recT 
                   Pseudomonas aeruginosa  39016 
                 MGTALTPLLTKFATRYEMGTTPEEVANTLKQTCFKGQVNDSQ 
                 24 
               
               
                   
                   
                   
                   
                 MVALLIVADQYKLNPFTKELYAFPDKNNGIVPVVGVDGWARII 
                   
               
               
                   
                   
                   
                   
                 NENPQFDGMEFSMDQQGTECTCKIYRKDRSHAISATEYMAEC 
                   
               
               
                   
                   
                   
                   
                 KRNTQPWQSHPRRMLRHKAMIQCARLAFGFAGIYDQDEAERI 
                   
               
               
                   
                   
                   
                   
                 VERDVTPAEQYEDVSEAICLIKDSPTMEDLQAAFSNAWKAYKT 
                   
               
               
                   
                   
                   
                   
                 KGARDQLTAAKDQRKKELLDAPIDVEFEETGDDRAA 
                   
               
               
                   
               
               
                 SR017 
                 SSAP 
                 gp2.5 
                   Salmonella  phage SETP3 
                 MGIKLNLRKVQTAWLNVFERAKDRENSDGSITKGTYNGTFILT 
                 25 
               
               
                   
                   
                   
                   
                 PEHPQIEELRDTVFAVVSEALGEAAAEKWMKQNYGEGKHMD 
                   
               
               
                   
                   
                   
                   
                 KCAVRDIAERDNPFEDFPEGFYFQAKNKQQPLILTSVKGEKQV 
                   
               
               
                   
                   
                   
                   
                 EPDFNIDGEQIEGKQVYSGCVANISIEIWFSEQYKVEGAKLNGIK 
                   
               
               
                   
                   
                   
                   
                 FAGEGKAFGGSAVSASVDDLEDDEDETPRRERRRNR 
                   
               
               
                   
               
               
                 SR018 
                 SSAP 
                 ERF 
                 
                   Paenibacillus dendritiformis 
                 
                 MGTEKLNIYQKLLEVRKSVSYLKKEETSQQYKYTGSAQVLAS 
                 26 
               
               
                   
                   
                   
                 C454 
                 VRDKINEMGLILVPRILDKSLLTETVEFIDKEKPKKTTTYFTELT 
                   
               
               
                   
                   
                   
                   
                 LSMTWVNADNPSETVECPWYSQGVDIAGEKGVGKALTYGEK 
                   
               
               
                   
                   
                   
                   
                 YFILKFFNIPTDKDDPDAFQKKFEQKASKEIQAKIRETWIKLGLK 
                   
               
               
                   
                   
                   
                   
                 INLLEHQCKTIYGSGLAQLSEEAAEEFLTMLEAKMGDPDGVN 
                   
               
               
                   
               
               
                 SR019 
                 SSAP 
                 recT 
                 
                   Enterococcus faecalis 
                 
                 MGNELIVSVQNRIQEMQHGEGLRLPTGYSVGNALNSAYLILSD 
                 27 
               
               
                   
                   
                   
                 TX0309B,  Enterococcus faecalis   
                 NSKGKSLLEKCHPTSVSKALLNMAIQGLSPAKNQCYFVPYGDQ 
                   
               
               
                   
                   
                   
                 TX0309A,  Enterococcus faecalis   
                 CTLMRSYFGSVSILERLSNVKKVHAEVIFEGDEFEIGSEDGRTV 
                   
               
               
                   
                   
                   
                 (strain ATCC 700802/V583) 
                 VTNFKPSFLNRDNPIIGAFAWVEQTDGIKVYTIMTKKEIYKSWS 
                   
               
               
                   
                   
                   
                   
                 KAKTKNVQNDYPQEMAKRTVLSRAAKMFINSSSDNDLLVKAI 
                   
               
               
                   
                   
                   
                   
                 NETTEDEYDNNQPRKDITPNPPNIEKLEKSIFNQDENKKIAQDMI 
                   
               
               
                   
                   
                   
                   
                 DSIDLNQADKDLQEELNIEFPDPSKNYLATGEVNGDVENEDGP 
                   
               
               
                   
                   
                   
                   
                 YPF 
                   
               
               
                   
               
               
                 SR020 
                 SSAP 
                 sak4 
                   Enterobacteria  phage 
                 MGTATLILGESGTGKSTSMRNINPEEAILIKPIGKPLPFKSKEWL 
                 28 
               
               
                   
                   
                   
                 HK629,  Salmonella  phage HK620 
                 AWDARAKKGTVVTTDKWDVIVAVIKRAHEYGKRIVIVDDFQY 
                   
               
               
                   
                   
                   
                 (Bacteriophage HK620) 
                 VMSNEFMRRSEEKSFDKFTEIGRHAWEVIKAAQDAPDDLRVYF 
                   
               
               
                   
                   
                   
                   
                 LAHTEETPMGRVKMKTIGKMLDEKITVEGMFTIVLRTLTRDDQ 
                   
               
               
                   
                   
                   
                   
                 FFFTTKNNGADTVKSPMGMFDSNEIDNDLSFVDATVCDYYGIN 
                   
               
               
                   
                   
                   
                   
                 NVHQIKENAA 
                   
               
               
                   
               
               
                 SR021 
                 SSAP 
                 recT 
                   Klebsiella pneumoniae  subsp 
                 MAGKLASRLGMDAGTDLMNTLKNTAFKGGNVTDDQFTALLI 
                 29 
               
               
                   
                   
                   
                 
                   rhinoscleromatis ATCC 13884 
                 
                 VANQYGLNPWTKEIYAFPDKGGIVPVVGVDGWVRIINEHPQFD 
                   
               
               
                   
                   
                   
                   
                 GMGFTYDKEEGACTCKIYRKDRTHPTIVTEYMGECKRNTQPW 
                   
               
               
                   
                   
                   
                   
                 QSHPTRMLRHKTLIQCARLAFGFAGIFDQDEAERVIEGSAAEVH 
                   
               
               
                   
                   
                   
                   
                 VGHESDSRRPELIAKGESAARLGTVKYQEFWVALSAEEKQVIG 
                   
               
               
                   
                   
                   
                   
                 AVEKRRMYDMSLAVDNAEPVDAAA 
                   
               
               
                   
               
               
                 SR022 
                 SSAP 
                 recT 
                   Clostridium sporogenes  (strain 
                 MAENKNSAVALLEKEMVYQVGEEEVKLTGSIVKNYLAKGNK 
                 30 
               
               
                   
                   
                   
                 ATCC 7955/DSM 767/NBRC 
                 QITNREVVVFMNLCKYRKLNPFLNEAYLVKFKDEAQIVTGKEA 
                   
               
               
                   
                   
                   
                 16411/NCIMB 8053/NCTC 
                 FMRKAEENPNYKGHRAGIIVMREKEIVELEGCFKLKTDTLLGG 
                   
               
               
                   
                   
                   
                 8594/PA 3679) 
                 WAEVMVEGKNCPIVAKVSLEEYNKQQSTWKSMPSTMIRKVAL 
                   
               
               
                   
                   
                   
                   
                 VQALREAFPAEIGAMYSNEELGVDESKIVNVQHEVKEEIKEEA 
                   
               
               
                   
                   
                   
                   
                 NKEVIDIEETELVEKETPVVEAEIVEPKDGEEETPY 
                   
               
               
                   
               
               
                 SR023 
                 SSAP 
                 ERF 
                   Staphylococcus  phage 
                 MAEQLNLYQKIADVKANIAGFTKDTKGYNFSYVSGSQILHRIR 
                 31 
               
               
                   
                   
                   
                 SA97,  Staphylococcus phage   
                 EKMIEHNLLLVPNTSNENWTTHTFKNKKGQEVTEFIVEMDLNY 
                   
               
               
                   
                   
                   
                 phi7247PVL,  Staphylococcus   
                 TWINADKPEEQYEVSYHAYGQQNDISQAHGTALTYAERYFLM 
                   
               
               
                   
                   
                   
                 phage phiETA3,  Staphylococcus   
                 KFFNIPTDEDDADAKQKQDKYSTVSQEFKDILTKEVNDFIAIAK 
                   
               
               
                   
                   
                   
                   aureus ,  Staphylococcus phage   
                 ESGFAEKYQEQINKLEKMNVEALNKNQINVTRQQIKKWLGGIE 
                   
               
               
                   
                   
                   
                 phi5967PVL 
                 Q 
                   
               
               
                   
               
               
                 SR024 
                 SSAP 
                 gp2.5 
                 
                   Paenibacillus lactis 154 
                 
                 MAIDNQSTKVITGKVRLSYTHVFEPQENDSGDMKYSTAILIPKS 
                 32 
               
               
                   
                   
                   
                   
                 DKETLRKIKAAVDAAKELGKSKWGGKIPANCKTPLRDGDEER 
                   
               
               
                   
                   
                   
                   
                 PDDEAYAGHYFLNASSKNKPGVAKPIGKDGNGKTKFQEITDST 
                   
               
               
                   
                   
                   
                   
                 EVYSGCYAKVSLNFYPFDAKGNRGVAAGLNNIVKVQDGDFLG 
                   
               
               
                   
                   
                   
                   
                 GRSSVNDDFANEDFDDIVDISDDDDFLN 
                   
               
               
                   
               
               
                 SR025 
                 SSAP 
                 recT 
                 
                   Desulfitobacterium 
                 
                 MAITPNPIPAQDGSPIPSPDDIVGELARRKIYAGIPDDDVALALA 
                 33 
               
               
                   
                   
                   
                   metallireducens  DSM 15288 
                 LCQKYGFDPLLKHLVLLATKDRDETTGQGQKHYNAYVTRDGL 
                   
               
               
                   
                   
                   
                   
                 LHVAHTSGMLDGLETIQGKDDLGEWAEAVVYRKDMSRPFRY 
                   
               
               
                   
                   
                   
                   
                 RVYLSEYVREAKGVWKTHPQAMLTKTAEVFALRRAFDVALTP 
                   
               
               
                   
                   
                   
                   
                 FEEMGFDNQNIAGDTGPSPKTGFTEKAGFTGNTDFSAEASLPG 
                   
               
               
                   
                   
                   
                   
                 KARFSTEAGLTDMTVIPPNRVTGSIPETSRLNTSAGSTGRQRRQ 
                   
               
               
                   
                   
                   
                   
                 LF 
                   
               
               
                   
               
               
                 SR026 
                 SSAP 
                 recT 
                 
                   Listeria monocytogenes 
                 
                 MATNDELKNQLANKQNGGQVASAQSLDLKGLLEAPTMRKKF 
                 34 
               
               
                   
                   
                   
                   
                 EKVLDKKAPQFLTSLLNLYNGDDYLQKTDPMTVVTSAMVAAT 
                   
               
               
                   
                   
                   
                   
                 LDLPIDKNLGYAWIVPYKGRAQFQLGYKGYIQLALRTGQYKSI 
                   
               
               
                   
                   
                   
                   
                 NVIEVRDGELLKWNRLTEEIELDLDNNTSEKVIGYCGYFQLING 
                   
               
               
                   
                   
                   
                   
                 FEKTVYWTRKEIEAHKKKFSKSDFGWKKDYDAMAKKTVLRN 
                   
               
               
                   
                   
                   
                   
                 MLSKWGILSIDMQTAVTEDEAEPRERKDVTEDESIPDIIDAPITP 
                   
               
               
                   
                   
                   
                   
                 SDTLEAGSEVQGSMI 
                   
               
               
                   
               
               
                 SR027 
                 SSAP 
                 recT 
                   Bacillus  phage SPP1 
                 MATKKQEELKNALAQQNGAVPQTPVKPQDKVKGYLERMMPA 
                 35 
               
               
                   
                   
                   
                 (Bacteriophage SPP1) 
                 IKDVLPKHLDADRLSRIAMNVIRTNPKLLECDTASLMGAVLES 
                   
               
               
                   
                   
                   
                   
                 AKLGVEPGLLGQAYILPYTNYKKKTVEAQFILGYKGLLDLVRR 
                   
               
               
                   
                   
                   
                   
                 SGHVSTISAQTVYKNDTFEYEYGLDDKLVHRPAPFGTDRGEPV 
                   
               
               
                   
                   
                   
                   
                 GYYAVAKMKDGGYNFLVMSKQDVEKHRDAFSKSKNREGVV 
                   
               
               
                   
                   
                   
                   
                 YGPWADHFDAMAKKTVLRQLLNYLPISVEQLSGVAADERTGSE 
                   
               
               
                   
                   
                   
                   
                 LHNQFADDDNIINVDINTGEIIDHQEKLGGETNE 
                   
               
               
                   
               
               
                 SR028 
                 SSAP 
                 recT 
                   Haemophilus influenzae , 
                 MATALQTLTNKLADRFDMGDGTGLTDVLTNTAFRGQKVSQD 
                 36 
               
               
                   
                   
                   
                   Haemophilus influenzae  NT127 
                 QMTALLVVANQYGLNPWTNEVYAFPNNGGIVPIVGVDGWARI 
                   
               
               
                   
                   
                   
                   
                 MNEHPQYDGMDFSFSEKGDSCTCTIYRKDRSRPIIVTEYMAEC 
                   
               
               
                   
                   
                   
                   
                 QRNTQPWKSHPKRMLRHKAMIQCARLAFGFTGIYDQDEAERI 
                   
               
               
                   
                   
                   
                   
                 VETKDPINVTPQPTVDETQAVELITPEQIEQITQLVEVTQSNMTQ 
                   
               
               
                   
                   
                   
                   
                 LLAAAGRAPSEEKVTKANAKHVIEKLLTKLDKQQAQDEQLGE 
                   
               
               
                   
                   
                   
                   
                 DVPIC 
                   
               
               
                   
               
               
                 SR029 
                 SSAP 
                 recT 
                   Clostridium botulinum  C str 
                 MANLMEIENKFEVNGAEVKLTGSIVKNYLTRGNDAVSDQEVV 
                 37 
               
               
                   
                   
                   
                 Eklund 
                 MFINLCKYQKLNPFLNEAYLVKFKGSPAQIITSKEAYMKKAER 
                   
               
               
                   
                   
                   
                   
                 NTNFAGMKAGIIVQRDKEILELEGSFCLKTDILLGGWAEVYKK 
                   
               
               
                   
                   
                   
                   
                 DREFPYKAKINLDEYDKGQSTWKKMPKTMIRKTAIVQALREAF 
                   
               
               
                   
                   
                   
                   
                 PEDLGAMYVEEEQQYQQDMSVEIKEEIKEKGNSKPLTLNPKTT 
                   
               
               
                   
                   
                   
                   
                 ENVQNVQEVKVEEVEIIN 
                   
               
               
                   
               
               
                 SR030 
                 SSAP 
                 gp2.5 
                   Synechococcus  phage Syn5 
                 MANRYVFNTTLEGFINVYEDSGKFNNRTFAYKFDAATLEQAE 
                 38 
               
               
                   
                   
                   
                   
                 KDREELLKWAKSKATGRVQEAMTPWDDEGLCKYTYGAGDGS 
                   
               
               
                   
                   
                   
                   
                 RKGKPEPIFVDSDGEVIDRNVLKDVRRGTKVRLIVQQKPYSMG 
                   
               
               
                   
                   
                   
                   
                 PNVGTSLRVLGVQIIELATGNGAVDSGDLSVDDVAALFGKADG 
                   
               
               
                   
                   
                   
                   
                 YKASEPAVRKAEDTVGDGDSYDF 
                   
               
               
                   
               
               
                 SR031 
                 SSAP 
                 recT 
                   Peptoniphilus duerdenii  ATCC 
                 MANIVKYETSNGEVQLDKQTIKNYLVSGDADKVTDQELELFIN 
                 39 
               
               
                   
                   
                   
                 BAA-1640 
                 LCKYQKLNPFLRDAYLVKFGDKPANMIVGKDFFIKRASANENF 
                   
               
               
                   
                   
                   
                   
                 KGYTAGVIVLGKTGNIEERPGSFYAKQVESLVGAWCKVEFTNG 
                   
               
               
                   
                   
                   
                   
                 TDFYHTVAFDEYNTGKSTWASKPATMIRKVALVQALREAFPE 
                   
               
               
                   
                   
                   
                   
                 DYQGLYDSSEMGVNEEVLPTDGVKVKAPTISKEQFNLLLKTLG 
                   
               
               
                   
                   
                   
                   
                 EDAIVDFCKSKGYNDAAKIKVDEYEKLIAEATKKKEEDEEVIE 
                   
               
               
                   
                   
                   
                   
                 YEDVDPDFKDLQDEDNNFEINEEELPF 
                   
               
               
                   
               
               
                 SR032 
                 SSAP 
                 recT 
                   Paenibacillus mucilaginosus  3016 
                 MAAPAKATDQKDLSKALANKAAAGNGQGKTIAQLFDEMKPAI 
                 40 
               
               
                   
                   
                   
                   
                 AQAIPKHLTPERLERIATTSIRTNPKLKVCTPESLLGAVMQCAQ 
                   
               
               
                   
                   
                   
                   
                 LGLEPSILGHAYLVPYRNKKKEGNKEYFVDEAQFQIGYKGLIEL 
                   
               
               
                   
                   
                   
                   
                 ARRTGHISSIMSQAVHEKDLFEYEYGINEKLRHVPADGDRGPV 
                   
               
               
                   
                   
                   
                   
                 TKYVAYAKFKDGGYSFMVMSKRDIELHRDKFSKAKFGPWVD 
                   
               
               
                   
                   
                   
                   
                 HFDEMAKKTVLKALMKYMPISVEFQKAVSMDETTKREVSDD 
                   
               
               
                   
                   
                   
                   
                 MSEVIDVTDWSESSAEDAGGGDEQRDPDTGLLNDRPPDDQVE 
                   
               
               
                   
                   
                   
                   
                 FE 
                   
               
               
                   
               
               
                 SR033 
                 SSAP 
                 rad52 
                   Lactococcus  phage 
                 MADYEEQMLALQKPLQPDRVVWRVQQSGFSKQGKPWAMVL 
                 41 
               
               
                   
                   
                   
                 bIL286,  Lactococcus lactis  subsp 
                 AYMDNRAVQERFDEVFGIAGWKNEFKTAPDGGTECGISVKFG 
                   
               
               
                   
                   
                   
                   lactis  (strain IL1403) 
                 DEWVTKWDGAENTQVEAVKGGLSGSMKRAAVQWGVGRYLY 
                   
               
               
                   
                   
                   
                 ( Streptococcus lactis ) 
                 DLPTSFAQTSLEKTDGWNKVFDKNSKKNFWWKNPQLPSWALP 
                   
               
               
                   
                   
                   
                   
                 QNSKVQNTKADFTEEEIPNPPKLYVVGKDKKEFDEKKLQAVV 
                   
               
               
                   
                   
                   
                   
                 NKMAIIAGKDYGASVDEQNDWLKMPLDEAYNDIEKFVDIKKE 
                   
               
               
                   
                   
                   
                   
                 EQND 
                   
               
               
                   
               
               
                 SR034 
                 SSAP 
                 other 
                   Drosophila melanogaster  (Fruit 
                 MASNNSSTTDLDSQVNVEDLPITEKVKYIGSEVARGLWGIKYT 
                 42 
               
               
                   
                   
                   
                 fly) 
                 RRPVDIMVGVAKNLPPNKVLPNCELKVSTDGVQLEIISPKASIN 
                   
               
               
                   
                   
                   
                   
                 HWSYPIDTISYGVQDLVYTRVFAMIVVKDESSPHPFEVHAFVC 
                   
               
               
                   
                   
                   
                   
                 DSRAMARKLTFALAAAFQDYSRRVKEATGEEEGEATPSDTITP 
                   
               
               
                   
                   
                   
                   
                 TRHKFAIDLRTPEEIQAGELEQETEA 
                   
               
               
                   
               
               
                 SR035 
                 SSAP 
                 gp2.5 
                   Burkholderia  phage BcepNazgul 
                 MAREIKFKGKNVVVFTDGTMRIENVRASYPHLAKPYKGDDQE 
                 43 
               
               
                   
                   
                   
                   
                 GQEKYSLVGFIEKDLAKSVLEVMKKMRDEMLREKNDGKKIPT 
                   
               
               
                   
                   
                   
                   
                 DKFFFRDGDASGKDEYEGCYTINASETKRPSVRGADKRLLGER 
                   
               
               
                   
                   
                   
                   
                 EIENTIYAGCRVNILINPWWQDNKFGKRINANELAVQFVRDDE 
                   
               
               
                   
                   
                   
                   
                 PIGEGRISEDEIDDSFDDVSDGEEALAEGYDDNGGL 
                   
               
               
                   
               
               
                 SR036 
                 SSAP 
                 recT 
                 
                   Legionella pneumophila 
                 
                 MATQKVDKRSMMVANQKTVMGLLEQMKGEIARCEPKHLTPE 
                 44 
               
               
                   
                   
                   
                   
                 RMARIAMTELRKTPKLQECDPLSFIASIMQAAQEGLEPGILGSC 
                   
               
               
                   
                   
                   
                   
                 YLIPFWNSKLGKFECTFMPGYRGFLDLARRSGQIVSLVARSVYE 
                   
               
               
                   
                   
                   
                   
                 NDEFSYEFGLKENIIHKPAMDNKGQLIAVYAVAILKDGGHQFD 
                   
               
               
                   
                   
                   
                   
                 VMSKEEVDTVRETSKSKDNGPWVTHYEEMAKKTVLRRLFKW 
                   
               
               
                   
                   
                   
                   
                 LPCSVEMQKAVSLDEMQEAGMQNIKVAASEEFDIDFVIDADTG 
                   
               
               
                   
                   
                   
                   
                 EVTEIPGNKSREDLKALIEKNKAESKNSQEKETNQDKA 
                   
               
               
                   
               
               
                 SR037 
                 SSAP 
                 recT 
                   Lactococcus  phage 
                 MANELGIFSVDNLNMTTIKQYLDGGGKASDAELVLLINLCKQN 
                 45 
               
               
                   
                   
                   
                 phi311,  Lactococcus  phage 
                 NMNPFMKEVYFIKYGNQPAQIVVSRDFYRKRAFQNPNFVGIEV 
                   
               
               
                   
                   
                   
                 ul36k1t1,  Lactococcus  phage 
                 GVIVLNKDGVLEHNEGTFKTHEQELVGAWARVHLKNTEIPVY 
                   
               
               
                   
                   
                   
                 ul362,  Lactococcus  phage 
                 VAVSYDEYVQMKDGHPNKMWTNKPCTMLGKVAESQALRMA 
                   
               
               
                   
                   
                   
                 ul361,  Lactococcus lactis , 
                 FPAEFSGTYGEEEYPEPEKEPREVNGVKEPDRAQIESFDKEDYA 
                   
               
               
                   
                   
                   
                   Lactococcus  phage ul36k1 
                 AKKIEELKEKAQPQKEVVEETGEVIDEEPLEGF 
                   
               
               
                   
               
               
                 SR038 
                 SSAP 
                 recT 
                   Streptococcus  phage M102 
                 MAKNELAKGSYLTDLQKLDGNTLRDFVDPKHQASPQELQALL 
                 46 
               
               
                   
                   
                   
                   
                 AIVKGRNLNPFTKEVYFIKYGSAPAQIVVSKEAIMKRAEENPDF 
                   
               
               
                   
                   
                   
                   
                 DGFEAGIVVETKDGAIERLTGTIVPKSATLRGGWCKVYRKDRS 
                   
               
               
                   
                   
                   
                   
                 HAIEADADFAYYTTSKNLWQKMPALMIRKVAIVSAFREAFSES 
                   
               
               
                   
                   
                   
                   
                 VGGLYTADEMQRETQAEVRARKMKEAYEEKLYELTQMEAKS 
                   
               
               
                   
                   
                   
                   
                 YKKTKSKNENEAKKTKEAEAIETVEEPTQDGNLEW 
                   
               
               
                   
               
               
                 SR039 
                 SSAP 
                 ERF 
                   Streptococcus  phage MM1 
                 MADLTFAELQRKMQIEKQTKQGVKYPFRTAEDINNKFKSLDSG 
                 47 
               
               
                   
                   
                   
                 1998,  Streptococcus pneumoniae , 
                 WSVSDPEDDIIQKGDKLYYKAVAVVKRESDGTIEKAIGWAREE 
                   
               
               
                   
                   
                   
                   Streptococcus phage  MM1 
                 DVPIFHTQKGDVKQMQDPQWTGAVGSVARKYALQGLFAIGGE 
                   
               
               
                   
                   
                   
                   
                 DVDEYPVEESQEQGQNNQQQKPNNQQAQGQNQVRYIDNTQY 
                   
               
               
                   
                   
                   
                   
                 QEINDLINDIAKIKGMPFDTLANYVLSEKLKGLQDFHRVQVGD 
                   
               
               
                   
                   
                   
                   
                 YEVLKNYLTEQLAKAKAKAKRGN 
                   
               
               
                   
               
               
                 SR040 
                 SSAP 
                 recT 
                   Paenibacillus elgii  B69 
                 MAQNKAIQTIDTQAVVGSFTQSELDTLKNTIAKGTTNEQFSLEV 
                 48 
               
               
                   
                   
                   
                   
                 QTCARSGLNPFLNQIYCIVYNTRDHGPTMSIQIAVEGIVALAKR 
                   
               
               
                   
                   
                   
                   
                 HPQYKGFIASEVKENDEFQIDVVSGEPVHKIKTLQRGKTIGAYC 
                   
               
               
                   
                   
                   
                   
                 VAYREGAPNISVIVTLDQIEHLLKGRNATMWKDYEDDMIVKH 
                   
               
               
                   
                   
                   
                   
                 AIKRAFKRQFGIEVAEDEYIPSGPNIDNIPEYQPQARRDITHEAE 
                   
               
               
                   
                   
                   
                   
                 LLAAAGRAPSLEKVTKANAKHVIEKLLTKLDKQQAQDEQLGE 
                   
               
               
                   
                   
                   
                   
                 DVPIC 
                   
               
               
                   
               
               
                 SR029 
                 SSAP 
                 recT 
                   Clostridium botulinum  C str 
                 MANLMEIENKFEVNGAEVKLTGSIVKNYLTRGNDAVSDQEVV 
                 37 
               
               
                   
                   
                   
                 Eklund 
                 MFINLCKYQKLNPFLNEAYLVKFKGSPAQIITSKEAYMKKAER 
                   
               
               
                   
                   
                   
                   
                 NTNFAGMKAGIIVQRDKEILELEGSFCLKTDILLGGWAEVYKK 
                   
               
               
                   
                   
                   
                   
                 DREFPYKAKINLDEYDKGQSTWKKMPKTMIRKTAIVQALREAF 
                   
               
               
                   
                   
                   
                   
                 PEDLGAMYVEEEQQYQQDMSVEIKEEIKEKGNSKPLTLNPKTT 
                   
               
               
                   
                   
                   
                   
                 ENVQNVQEVKVEEVEIIN 
                   
               
               
                   
               
               
                 SR030 
                 SSAP 
                 gp2.5 
                   Syncchococcus  phage Syn5 
                 MANRYVFNTTLEGFINVYEDSGKFNNRTFAYKFDAATLEQAE 
                 38 
               
               
                   
                   
                   
                   
                 KDREELLKWAKSKATGRVQEAMTPWDDEGLCKYTYGAGDGS 
                   
               
               
                   
                   
                   
                   
                 RKGKPEPIFVDSDGEVIDRNVLKDVRRGTKVRLIVQQKPYSMG 
                   
               
               
                   
                   
                   
                   
                 PNVGTSLRVLGVQIIELATGNGAVDSGDLSVDDVAALFGKADG 
                   
               
               
                   
                   
                   
                   
                 YKASEPAVRKAEDTVGDGDSYDF 
                   
               
               
                   
               
               
                 SR031 
                 SSAP 
                 recT 
                   Peptouiphilus duerdenii  ATCC 
                 MANIVKYETSNGEVQLDKQTIKNYLVSGDADKVTDQELELFIN 
                 39 
               
               
                   
                   
                   
                 BAA-1640 
                 LCKYQKLNPFLRDAYLVKFGDKPANMIVGKDFFIKRASANENF 
                   
               
               
                   
                   
                   
                   
                 KGYTAGVIVLGKTGNIEERPGSFYAKQVESLVGAWCKVEFTNG 
                   
               
               
                   
                   
                   
                   
                 TDFYHTVAFDEYNTGKSTWASKPATMIRKVALVQALREAFPE 
                   
               
               
                   
                   
                   
                   
                 DYQGLYDSSEMGVNEEVLPTDGVKVKAPTISKEQFNLLLKTLG 
                   
               
               
                   
                   
                   
                   
                 EDAIVDFCKSKGYNDAAKIKVDEYEKLIAEATKKKLEDEEVIE 
                   
               
               
                   
                   
                   
                   
                 YEDVDPDFKDLQDEDNNFEINEEELPF 
                   
               
               
                   
               
               
                 SR032 
                 SSAP 
                 recT 
                   Paenibacillus mucilaginosus  3016 
                 MAAPAKATDQKDLSKALANKAAAGNGQGKTIAQLFDEMKPAI 
                 40 
               
               
                   
                   
                   
                   
                 AQAIPKHLTPERLLRIATTSIRTNPKLKVCTPESLLGAVMQCAQ 
                   
               
               
                   
                   
                   
                   
                 LGLEPSLLGHAYLVPYRNKKKEGNKEYFVDEAQFQIGYKGLIEL 
                   
               
               
                   
                   
                   
                   
                 ARRTGHISSIMSQAVHEKDLFEYEYGINEKLRHVPADGDRGPV 
                   
               
               
                   
                   
                   
                   
                 TKYYAYAKFKDGGYSFMVMSKRDIELHRDKFSKAKFGPWVD 
                   
               
               
                   
                   
                   
                   
                 HFDEMAKKTVLKALMKYMPISVEFQKAVSMDETTKREVSDD 
                   
               
               
                   
                   
                   
                   
                 MSEVIDVTDWSESSAEDAGGGDEQRDPDTGLLNDRPPDDQVE 
                   
               
               
                   
                   
                   
                   
                 FE 
                   
               
               
                   
               
               
                 SR033 
                 SSAP 
                 rad52 
                   Lactococcus  phage 
                 MADYEEQMLALQKPLQPDRVVWRVQQSGFSKQGKPWAMVL 
                 41 
               
               
                   
                   
                   
                 bIL286,  Lactococcus lactis  subsp 
                 AYMDNRAVQERFDEVFGIAGWKNEFKTAPDGGTLCGISVKFG 
                   
               
               
                   
                   
                   
                   lactis  (strain IL1403) 
                 DEWVTKWDGAENTQVEAVKGGLSGSMKRAAVQWGVGRYLY 
                   
               
               
                   
                   
                   
                 ( Streptococcus lactis ) 
                 DLPTSFAQTSLEKTDGWNKVFDKNSKKNFWWKNPQLPSWALP 
                   
               
               
                   
                   
                   
                   
                 QNSKVQNTKADFTEEEIPNPPKLYVVGKDKKEFDEKKLQAVV 
                   
               
               
                   
                   
                   
                   
                 NKMAIIAGKDYGASVDEQNDWLKMPLDEAYNDIEKFVDIKKE 
                   
               
               
                   
                   
                   
                   
                 EQND 
                   
               
               
                   
               
               
                 SR034 
                 SSAP 
                 other 
                   Drosophila melanogaster  (Fruit 
                 MASNNSSTTDLDSQVNVEDLPITFKVKYIGSEVARGLWGIKYT 
                 42 
               
               
                   
                   
                   
                 fly) 
                 RRPVDIMVGVAKNLPPNKVLPNCELKVSTDGVQLEIISPKASIN 
                   
               
               
                   
                   
                   
                   
                 HWSYPIDTISYGVQDLVYIRVFAMIVVKDESSPHPFEVHAFVC 
                   
               
               
                   
                   
                   
                   
                 DSRAMARKLTFALAAAFQDYSRRVKEATGEEEGEATPSDTITP 
                   
               
               
                   
                   
                   
                   
                 TRHKFAIDLRTPEEIQAGELEQETEA 
                   
               
               
                   
               
               
                 SR035 
                 SSAP 
                 gp2.5 
                   Burkholderia  phage BcepNazgul 
                 MAREIKFKGKNVVVFTDGTMRIENVRASYPHLAKPYKGDDQE 
                 43 
               
               
                   
                   
                   
                   
                 GQEKYSLVGFIEKDLAKSVLEVMKKMRDEMLREKNDGKKIPT 
                   
               
               
                   
                   
                   
                   
                 DKFFFRDGDASGKDEYEGCYTLNASETKRPSVRGADKRLLGER 
                   
               
               
                   
                   
                   
                   
                 EIENTIYAGCRVNILINPWWQDNKFGKRINANLLAVQFVRDDE 
                   
               
               
                   
                   
                   
                   
                 PIGEGRISEDEIDDSFDDVSDGEEALAEGYDDNGGL 
                   
               
               
                   
               
               
                 SR036 
                 SSAP 
                 recT 
                 
                   Legionella pneumophila 
                 
                 MATQKVDKRSMMVANQKTVMGLLEQMKGEIARCLPKHLTPE 
                 44 
               
               
                   
                   
                   
                   
                 RMARIAMTELRKTPKLQECDPLSFIASLMQAAQLGLEPGILGSC 
                   
               
               
                   
                   
                   
                   
                 YLIPFWNSKLGKFECTFMPGYRGFLDLARRSGQIVSLVARSVYE 
                   
               
               
                   
                   
                   
                   
                 NDEFSYEFGLKENIIHKPAMDNKGQLIAVYAVAILKDGGHQFD 
                   
               
               
                   
                   
                   
                   
                 VMSKEEVDTVRETSKSKDNGPWVTHYEEMAKKTVLRRLFKW 
                   
               
               
                   
                   
                   
                   
                 LPCSVEMQKAVSLDEMQEAGMQNIKVAASEEFDIDFVIDADTG 
                   
               
               
                   
                   
                   
                   
                 EVTEIPGNKSREDLKALIEKNKAESKNSQEKETNQDKA 
                   
               
               
                   
               
               
                 SR037 
                 SSAP 
                 recT 
                   Lactococcus  phage 
                 MANELGIFSVDNLNMTTIKQYLDGGGKASDAELVLLLNLCKQN 
                 45 
               
               
                   
                   
                   
                 phi311,  Lactococcus  phage 
                 NMNPFMKEVYFIKYGNQPAQIVVSRDFYRKRAFQNPNFVGIEV 
                   
               
               
                   
                   
                   
                 ul36k1t1,  Lactococcus  phage 
                 GVIVLNKDGVLEHNEGTFKTHEQELVGAWARVHLKNTEIPVY 
                   
               
               
                   
                   
                   
                 ul362,  Lactococcus  phage 
                 VAVSYDEYVQMKDGHPNKMWTNKPCTMLGKVAESQALRMA 
                   
               
               
                   
                   
                   
                 ul361,  Lactococcus  lactis, 
                 FPAEFSGTYGEEEYPEPEKEPREVNGVKEPDRAQIESFDKEDYA 
                   
               
               
                   
                   
                   
                   Lactococcus  phage ul36k1 
                 AKKIEELKEKAQPQKEVVEETGEVIDEEPLEGF 
                   
               
               
                   
               
               
                 SR038 
                 SSAP 
                 recT 
                   Streptococcus  phage M102 
                 MAKNELAKGSYLTDLQKLDGNTLRDFVDPKHQASPQELQALL 
                 46 
               
               
                   
                   
                   
                   
                 AIVKGRNLNPFTKEVYFIKYGSAPAQIVVSKEAIMKRAEENPDF 
                   
               
               
                   
                   
                   
                   
                 DGFEAGIVVETKDGAIERLTGTIVPKSATLRGGWCKVYRKDRS 
                   
               
               
                   
                   
                   
                   
                 HAIEADADFAYYTTSKNLWQKMPALMIRKVAIVSAFREAFSES 
                   
               
               
                   
                   
                   
                   
                 VGGLYTADEMQRETQAEVRARKMKEAYEEKLYLLTQMEAKS 
                   
               
               
                   
                   
                   
                   
                 YKKTKSKNENEAKKTKEAEAIETVEEPITQDGNLEW 
                   
               
               
                   
               
               
                 SR039 
                 SSAP 
                 ERF 
                   Streptococcus  phage MM1 
                 MADLTFAELQRKMQIEKQTKQGVKYPFRTAEDINNKFKSLDSG 
                 47 
               
               
                   
                   
                   
                 1998,  Streptococcus pneumoniae , 
                 WSVSFPEDDIIQKGDKLYYKAVAVVKRESDGTIEKAIGWAREE 
                   
               
               
                   
                   
                   
                   Streptococcus  phage MM1 
                 DVPIFHTQKGDVKQMQDPQWTGAVGSYARKYALQGLFAIGGE 
                   
               
               
                   
                   
                   
                   
                 DVDEYPVEESQEQGQNNQQQKPNNQQAQGQNQVRYIDNTQY 
                   
               
               
                   
                   
                   
                   
                 QEINDLLNDIAKIKGMPFDTLANYVLSEKLKGLQDFHRVQVGD 
                   
               
               
                   
                   
                   
                   
                 YEVLKNYLTEQLAKAKAKAKRGN 
                   
               
               
                   
               
               
                 SR040 
                 SSAP 
                 recT 
                   Paenibacillus elgii  B69 
                 MAQNKAIQTIDTQAVVGSFTQSELDTLKNTIAKGTTNEQFSLFV 
                 48 
               
               
                   
                   
                   
                   
                 QTCARSGLNPFLNQIYCIVYNTRDHGPTMSIQIAVEGIVALAKR 
                   
               
               
                   
                   
                   
                   
                 HPQYKGFIASEVKENDEFQIDVVSGEPVHKIKTLQRGKTIGAYC 
                   
               
               
                   
                   
                   
                   
                 VAYREGAPNISVIVTLDQIEHLLKGRNATMWKDYLDDMIVKH 
                   
               
               
                   
                   
                   
                   
                 AIKRAFKRQFGIEVAEDEYIPSGPNIDNIPEYQPQARRDITHEAE 
                   
               
               
                   
                   
                   
                   
                 QMNNEKEQPASPQPSEDDEAAKIKKARTEMKKKFAQLGITDPE 
                   
               
               
                   
                   
                   
                   
                 EISAYIAKNAKIKGEKPTLAEYTGLLKIMDMEIERRKAEQANDD 
                   
               
               
                   
                   
                   
                   
                 DLLMD 
                   
               
               
                   
               
               
                 SR041 
                 SSAP 
                 gp2.5 
                   Burkholderia  phage BcepC6B 
                 MAKIKLTNVRIAFINNLRTPAEFEAGDGKFRYSATFLVEKGSAN 
                 49 
               
               
                   
                   
                   
                   
                 DKAIEAAIKSVAVEGWAKKADAMLESFRSNSNKFCYQNGDLK 
                   
               
               
                   
                   
                   
                   
                 DFDGFEGNMYIAAHRKRDDGRPLLLDNVADPETGKPARLVDA 
                   
               
               
                   
                   
                   
                   
                 NGEWLAGKEGRIYAGCYVNATIDIYAQTKTNPGIRCGLMGVQ 
                   
               
               
                   
                   
                   
                   
                 YHGPGDSFSGASRANEDDFEATAPETEDELD 
                   
               
               
                   
               
               
                 SR042 
                 SSAP 
                 gp2.5 
                   Yersinia  phage YpsP-G,  Yersinia   
                 MAKKIFTSALGTAEPYAYIAKPDYGNEERGFGNPRGVYKVDLT 
                 50 
               
               
                   
                   
                   
                 phage phiA1122 
                 IPNKDPRCQRMVDEIVKCHEEAYAAAVEEYEANPPAVARGKK 
                   
               
               
                   
                   
                   
                   
                 PLKPYEGDMPFFDNGDGTTTFKFKCYASFQDKKTKETKHLNLV 
                   
               
               
                   
                   
                   
                   
                 VVDSKGKKMEDVPIIGGGSKLKVKYSLVPYKWNTAVGASVKL 
                   
               
               
                   
                   
                   
                   
                 QLESVMLVELATFGGGEDDWADEVEENGYVASGSAKASKPRD 
                   
               
               
                   
                   
                   
                   
                 EESWDEDDGDSYEEDSDGDF 
                   
               
               
                   
               
               
                 SR043 
                 SSAP 
                 recA 
                   Prochlorococcus  phage P-SSM2 
                 MDFLKEIVKEIGDEYTQVAADIQENERFIDTGSYIFNGLVSGSIF 
                 51 
               
               
                   
                   
                   
                   
                 GGVSSSRITAIAGESSTGKTYFSLAVVKNFLDNNPDGYCLYFDT 
                   
               
               
                   
                   
                   
                   
                 EAAVNKGLLESRGIDMNRLVVVNVVTIEEFRSKALRAVDIYLK 
                   
               
               
                   
                   
                   
                   
                 TSEEERKPCMFVLDSLGMLSTEKEIRDALDDKQVRDMTKSQLV 
                   
               
               
                   
                   
                   
                   
                 KGAFRMLTLKLGQANIPLIVTNHTYDVIGSYVPTKEMGGGSGL 
                   
               
               
                   
                   
                   
                   
                 KYAASTIIYLSKKKEKDQKEVIGNLIKAKTHKSRLSKENKEVQI 
                   
               
               
                   
                   
                   
                   
                 RLYYDERGLDRYYGLLELGEIGGMWKNVAGRYEMNGKKIYA 
                   
               
               
                   
                   
                   
                   
                 KEILKNPTEYFTDDIMEQLDNIAKEIIFSYGTN 
                   
               
               
                   
               
               
                 SR044 
                 SSAP 
                 rad52 
                   Salmonella typhimurium , 
                 MDLNKFDAPFNPEDIEWRIQQSGKTRDGKVWAMVLAYVTNR 
                 52 
               
               
                   
                   
                   
                   Salmonella  phage 
                 AIMKRLDDVCGKEGWRNEYRDIPNNGGVECGISIKIDSEWVTK 
                   
               
               
                   
                   
                   
                 ST160,  Salmonella  phage ST64T 
                 WDAAENTQVEAVKGGRSGAMKRAAVQWGIGRYLYNLEEGFA 
                   
               
               
                   
                   
                   
                 (Bacteriophage ST64T) 
                 QISSDKKQGWHRAKLKDGTGFYWLPPSLPDWAMPASCNQPSP 
                   
               
               
                   
                   
                   
                   
                 ENTNQKSPSVDCEQILKDFSDYAATETDKKKLIERYQHDWQLL 
                   
               
               
                   
                   
                   
                   
                 AGHDDAQTRCVQVMNIRINELKQVA 
                   
               
               
                   
               
               
                 SR045 
                 SSAP 
                 gp2.5 
                   Salmonella  phage SS3c 
                 MDKCAVRDIAERDNPFEDFPEGFYFQAKNKQQPLILTSVKGEK 
                 53 
               
               
                   
                   
                   
                   
                 QVEPDFNIDGEQIEGEQVYSGCVANISIEIWFSEQYKVLGAKLN 
                   
               
               
                   
                   
                   
                   
                 GIKFAGEGKAFGGSAVSASVDDLEDDEDETPRRERRRNR 
                   
               
               
                   
               
               
                 SRQ46 
                 SSAP 
                 recT 
                   Bacteroides caccae  ATCC 43185 
                 MEENKLTKQENDALAIFGKGKTIYQVAGNDVALSFDIVRNYLT 
                 54 
               
               
                   
                   
                   
                   
                 KGNGQVSDQDIVQFISICKFNQLNPFLNEAFLVKFGQQPAQMIV 
                   
               
               
                   
                   
                   
                   
                 SKEAFFKRADASEKYEGFKAGIIIIRDNKIVEVEGCFYNEKTDIL 
                   
               
               
                   
                   
                   
                   
                 VGGWCEVYRSDRRFPIIAKVNLAEYDKKQSIWNEKKSTMISKI 
                   
               
               
                   
                   
                   
                   
                 AKVQALREAFPAQLGAMYTQEEQEVKFAEYEDVTDKESKANK 
                   
               
               
                   
                   
                   
                   
                 LAEIALKNAGVEEQPKTEQPVNQPQNNTNDKPAQKTLL 
                   
               
               
                   
               
               
                 SRQ47 
                 SSAP 
                 ERF 
                   Lactobacillus  prophage 
                 MEIYGKEEDRAKWAMHYAQVKANIKQPEKTHKVTVSGKTKQ 
                 55 
               
               
                   
                   
                   
                 Lj928,  Lactobacillus johnsonii   
                 GTPYSYDYNYADLNDIDAAVMDGIKKVTDKDGNVVFSYFFDI 
                   
               
               
                   
                   
                   
                 (strain CNCM I-12250/La1/ 
                 RTENNTVEVQTILVDSSGFTLKTNKVVFQNNKAWDAQATASLI 
                   
               
               
                   
                   
                   
                 NCC 533) 
                 SYAKRYSLSGAFGIAADNDDDAQDQKTIYEPKILTKQELEDYK 
                   
               
               
                   
                   
                   
                   
                 VYYNGIQANLYDLYQEAKDGIKDAQDWLKEPHTPQDAQAVH 
                   
               
               
                   
                   
                   
                   
                 QIAEMFKQNHSVKQETKEKQAAIDKIRQTVKKDPIEDKKVESK 
                   
               
               
                   
                   
                   
                   
                 DPEVDKLF 
                   
               
               
                   
               
               
                 SR048 
                 SSAP 
                 sak4 
                   Burkholderia  phage BcepGomr 
                 MECEMPLNWTTTANAARNGVKVLCYAPAGYGKTVLGATAPR 
                 56 
               
               
                   
                   
                   
                   
                 PIILSSEFGNLSIRKHNIPVLEIRNGLDMRDAYDWIMKSNEFKQH 
                   
               
               
                   
                   
                   
                   
                 FDTLVWDGASESAETFLRVAKASGGDGRAHYGTVADMIADYF 
                   
               
               
                   
                   
                   
                   
                 TKFRALPEKHVYITAKMGFVQDSVTGGVKYGPDFPGKQLGQQ 
                   
               
               
                   
                   
                   
                   
                 SPYWLDEVFTLRIGTLPDGTGTYRYLQTQPDPQYDAKDRSGSL 
                   
               
               
                   
                   
                   
                   
                 DAMSSRT 
                   
               
               
                   
               
               
                 SR049 
                 SSAP 
                 FRF 
                   Lactococcus  phage c2 
                 MESKVLKLINEIKVPKSQYNSFGKYNFRNNEDIQTALKPLLLQF 
                 57 
               
               
                   
                   
                   
                   
                 GLMEKATTEMLEMNNELMLHVHIDIFDPDNPNDIASGDGWAV 
                   
               
               
                   
                   
                   
                   
                 IDINKKGMDKAQATGASQSYASKYAYGQALKLDDTKDADSTN 
                   
               
               
                   
                   
                   
                   
                 KGPNNATQMKSRPKSNYQYNLSDLKKKVANKEISSDQANELC 
                   
               
               
                   
                   
                   
                   
                 KQGKVNMNA 
                   
               
               
                   
               
               
                 SR050 
                 SSAP 
                 recT 
                   Fusobacterium ulcerans  12-1B 
                 MEVKNNLVKTEEKKNVVNFTVDGMDVKLSPAIIRSYLVNGNG 
                 58 
               
               
                   
                   
                   
                   
                 AVTEQEINYFIQLCKARQLNPHVKDAYLIKYGSQPATMVVSKD 
                   
               
               
                   
                   
                   
                   
                 AIERRAIKHPQYNGKKVGIYVLDKNNELVKREHSILLEDLKLV 
                   
               
               
                   
                   
                   
                   
                 GAWCEVYRKDWEFPAKADVNYEEYVGRKSDGTVNSQWANK 
                   
               
               
                   
                   
                   
                   
                 PVTMLTKVAKAQALREAFIEELGGMYDSSEMSKDIPNIEEIIEAP 
                   
               
               
                   
                   
                   
                   
                 KQDLEMKNFIEDNKEEIKDKQEKILKEAEKVEEDELIQEIFGKK 
                   
               
               
                   
               
               
                 SR051 
                 SSAP 
                 ERF 
                   Streptococcus  phage 7201 
                 MEEMTFTELQQKIQLEKKKEGTAKYASRHVEDIYNVFKNLKSN 
                 59 
               
               
                   
                   
                   
                   
                 WNVVVNYELVEFSGKTFIKAIATASNKDEKVQAQAFAELSPVP 
                   
               
               
                   
                   
                   
                   
                 ILKTRNGELKQMNEPQWVGAVQSYAGKYALQALFAIGEEDVD 
                   
               
               
                   
                   
                   
                   
                 HFEVAEQSMRPNQNHNQMQSHQQANYINQQQHNQINQLIDEL 
                   
               
               
                   
                   
                   
                   
                 AKVTGQPVETVARYYLGKYKLNNFSELLTTGFDILANDIQDKI 
                   
               
               
                   
                   
                   
                   
                 KQRKG 
                   
               
               
                   
               
               
                 SR052 
                 SSAP 
                 ERF 
                   Lactobacillus  prophage 
                 MELLNQPTPPISSPQAVGLMNIQTIVGMDAKQSLDAKLSLLNDF 
                 60 
               
               
                   
                   
                   
                 Lj965,  Lactobacillus johnsonii   
                 VEMKRVLAQPSKSKDGYGYKYADLNDVLSVIQQAIGDLDLSFI 
                   
               
               
                   
                   
                   
                 (strain CNCM I-12250/La1/ 
                 QQPINKTAKTGVENYVFNSKGAILDFGSYMLDITKPQAQQYGS 
                   
               
               
                   
                   
                   
                 NCC 533) 
                 ALTYCRRYSISSIFGIASEEDTDAKALPQYMSPEEIDRLTLPYKG 
                   
               
               
                   
                   
                   
                   
                 KQVSLAKLFSLGLAGDSKAKAKLLDRENNNVTKLAVKSMTD 
                   
               
               
                   
                   
                   
                   
                 MWDFSKDIEAMKINEQTEIKASKDQEEKAKQAALNKVQKGKK 
                   
               
               
                   
                   
                   
                   
                 DPFEDKKVESDSDPEVDKLF 
                   
               
               
                   
               
               
                 SR053 
                 SSAP 
                 ERF 
                   Clostridium  phage 
                 METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP 
                 61 
               
               
                   
                   
                   
                 phiC2,  Peptoclostridium   
                 ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKAL 
                   
               
               
                   
                   
                   
                   difficile  E15,  Clostridium   
                 AREDETKKGMDLAQVTGSVSSYARKYALNGLFCIDDTKDSDA 
                   
               
               
                   
                   
                   
                 phage phiMMP03, 
                 TNKHGNEQKKKEVNESELNTLYSLGESIEKDKNRVDSEVYKKF 
                   
               
               
                   
                   
                   
                   Peptoclostridium   difficile   
                 GKLAVDETKQEYEKVENGYKSILEKQKQE 
                   
               
               
                   
                   
                   
                 ( Clostridium difficile ) 
                   
                   
               
               
                   
               
               
                 SR054 
                 SSAP 
                 recT 
                 [ Clostridium ]  methylpentosum   
                 MEKGKQVLAEKKPEENIRLDENNIFTGFREFQVAQRMATALAS 
                 62 
               
               
                   
                   
                   
                 DSM 5476 
                 STIVPKDYQNNPGNCLIALEMANRLKTSPMMVMQNLYVVNGR 
                   
               
               
                   
                   
                   
                   
                 PAWSSQYIVAMINSSRKYKTELQYEMKGSRTDGSLECTAWVE 
                   
               
               
                   
                   
                   
                   
                 DYNGHRVTGPTVTMKMAQEEGWIGRNGSKWKTMPEVMIRYR 
                   
               
               
                   
                   
                   
                   
                 AASFFGRLNCPDMIMGIYSEEEAIELEPSQFEFVDQVKAAEQEIK 
                   
               
               
                   
                   
                   
                   
                 DNVGRQDIDVDVHTGEVITKPTAQTQVEEESGEPDLETQMSIEE 
                   
               
               
                   
                   
                   
                   
                 PDF 
                   
               
               
                   
               
               
                 SR055 
                 SSAP 
                 recT 
                   Vibrio cholerae  (strain 
                 MEKPKLIQRFAERFSVDPNKLFDTLKATAFKQRDGSAPTNEQM 
                 63 
               
               
                   
                   
                   
                 MO10),  Vibrio cholerae , 
                 MALLVVADQVGLNPFTKEIFAFPDKQAGIIPVVGVDGWSRIINQ 
                   
               
               
                   
                   
                   
                 
                   Providencia alcalifaciens 
                 
                 HDQFDGMEFKTSENKVSLDGAKECPEWMECIIYRRDRSHPVKI 
                   
               
               
                   
                   
                   
                 Ban1,  Vibrio cholerae  Ind4 
                 TEYLDEVYRPPFEGNGKNGPYRVDGPWQTHTKRMERHKSMIQCS 
                   
               
               
                   
                   
                   
                   
                 RIAFGFVGIFDQDEAERIIEGQATHIVEPSVIPPEQVDDRTRG 
                   
               
               
                   
                   
                   
                   
                 LVYKLIERAEASNAWNSALEYANEHFQGVELTFAKQEIFNAQQ 
                   
               
               
                   
                   
                   
                   
                 QAAKALTQPLAS 
                   
               
               
                   
               
               
                 SR056 
                 SSAP 
                 sak4 
                   Listeria welshimeri  serovar 6b 
                 MLEFIQSEEMKRSEVFNIMIYAKPGAGKTTTIKYLKGKTLMLD 
                 64 
               
               
                   
                   
                   
                 (strain ATCC 35897/DSM 20650/ 
                 CDGTSKVLSGLPDITIATLNPRNPVQDMADFYGYAKTHADEYD 
                   
               
               
                   
                   
                   
                 SLCC5334),  Listeria  phage PSA 
                 NVVIDNLSHYQKLWLMFNGRNTKSGQPELQHVGIFDTHLIDMI 
                   
               
               
                   
                   
                   
                   
                 SVFNNLANTNIVYTAWENTRQIQLESGQLYNQFLPDIREKVVN 
                   
               
               
                   
                   
                   
                   
                 HVMGIVPVVARLIRNPETGQRGFLLTENNGNFAKNQLDNREFA 
                   
               
               
                   
                   
                   
                   
                 LQEDLFKIGDIDAKA 
                   
               
               
                   
               
               
                 SR057 
                 SSAP 
                 recT 
                 
                   Hydrogenobacter thermophilus 
                 
                 MLSKANTNTIVREEDYKKVLQMLRVALPSLAKVDDETLITAVA 
                 65 
               
               
                   
                   
                   
                 (strain DSM 6534/IAM 12695/ 
                 YARHLGLDPLRKEVHFVPYYNKELNKTIIQPIVSYTEYLKRAER 
                   
               
               
                   
                   
                   
                 TK-6) 
                 SGKLKGWSCKFRKEGDELVAVVEIKRVDWEIPFVWEVPLSEV 
                   
               
               
                   
                   
                   
                   
                 KRDTPLWQSHPLFMLKKTAIAQAFRMCFPEETSHLPYEEAEFW 
                   
               
               
                   
                   
                   
                   
                 EEPSIPEQTQPTKTDVDTDTESDYITEAQRKRFFAIVKKELGYRE 
                   
               
               
                   
                   
                   
                   
                 DAIKEILKERFGIESTAEIPKDKYEEIIDYFRSLSKPIEAEAQS 
                   
               
               
                   
               
               
                 SR058 
                 SSAP 
                 other 
                   Paenibacillus polymyxa  (strain 
                 
                   MLFRRRAASAFVRKVLYFAIICGLLWLLWLGLHGVMSTGEDD 
                 
                 66 
               
               
                   
                   
                   
                 E681) 
                 QAVEALNEFYTMEQSGNFGGSWEFFHSQMKKKFEKSTYVQQR 
                   
               
               
                   
                   
                   
                   
                 ARFYAGYGDGYVHV 
                   
               
               
                   
               
               
                 SR059 
                 SSAP 
                 sak4 
                   Streptococcus  phage Sfi21 
                 MRIIRAKDIQRTKNWRILIYGKAGLGKTSLIKNMPGKTLVLSLD 
                 67 
               
               
                   
                   
                   
                   
                 NSSKVLAGTENVDIIDFDREHPTEFITEFLTQADNLIKNYENLVI 
                   
               
               
                   
                   
                   
                   
                 DNISSFQSDWFIEQGRKSKNGISNELQHYSQWTNYFLRVLTVIY 
                   
               
               
                   
                   
                   
                   
                 SKPINIYVTAWEDTHELNLETGQILTQYVPQIRASVENQLLGLT 
                   
               
               
                   
                   
                   
                   
                 DVVGRIVVNAKTGARGLILEGSEGTYAKNRLDNRTACKIEDLF 
                   
               
               
                   
                   
                   
                   
                 KFGDLDGTKELPE 
                   
               
               
                   
               
               
                 SR060 
                 SSAP 
                 sak4 
                   Lactobacillus  phage phiadh 
                 MPAFKWDEDKGTKYRWLVYGVPGVGKTTLSKYLKGKTYLLS 
                 68 
               
               
                   
                   
                   
                   
                 LDESFHRIDFWKGKNDIWSIDPEKPIEDLADFVKAFKPDKYDNL 
                   
               
               
                   
                   
                   
                   
                 IIDNVSNLQKLFFIEKARETRTGLDNKMSDYNEWTTLITRFIAKC 
                   
               
               
                   
                   
                   
                   
                 FSWNINILVTAWEAQNKVTDPSGQEFMQVGPDIRPNPRDYILG 
                   
               
               
                   
                   
                   
                   
                 NCDVVARMVQKPQTGERGLIMQGSIDTYAKNRLDGRKGCKVE 
                   
               
               
                   
                   
                   
                   
                 DFFEVQDK 
                   
               
               
                   
               
               
                 SR061 
                 SSAP 
                 ERF 
                   Mycobacterium  phage PhatBacter, 
                 MPDEAQTGPLAEDPAPHTPTVFEAWSRVMSDVQAISKDSRNEQ 
                 69 
               
               
                   
                   
                   
                   Mycobacterium  phage 
                 QHFNFRGIDAVMNVVGPALRAHGVTVIPRAVEEYSERVETQPR 
                   
               
               
                   
                   
                   
                 Elph10,  Mycobacterium  phage 
                 GNRPGTPMINREVRVEFTVEGPRGDWFAGTTYGEAADSGDKA 
                   
               
               
                   
                   
                   
                 244,  Mycobacterium  phage 
                 MSKAHSVAYRTFLLQALTIPTDEPDPDEDVHERAPRQERRREP 
                   
               
               
                   
                   
                   
                 Cjw1,  Mycobacterium  phage 
                 KDEPPPLSDENAEGRAELREFCEENNLDAKVVAGKFATDNPGQ 
                   
               
               
                   
                   
                   
                 Phrux,  Mycobacterium  phage 
                 SIRTADNETIRAYIATMKAGLVKADV 
                   
               
               
                   
                   
                   
                 Lilac,  Mycobacterium  phage 
                   
                   
               
               
                   
                   
                   
                 Phaux,  Mycobacterium  phage 
                   
                   
               
               
                   
                   
                   
                 Quink,  Mycobacterium  phage 
                   
                   
               
               
                   
                   
                   
                 Pumpkin,  Mycobacterium  phage 
                   
                   
               
               
                   
                   
                   
                 Murphy 
                   
                   
               
               
                   
               
               
                 SR062 
                 SSAP 
                 recT 
                   Cryptobacterium curtum  (strain 
                 MPQEIAKVEYTAADGQEVRLTPGVIAKYIVSGNGLASEKDIVSF 
                 70 
               
               
                   
                   
                   
                 ATCC 700683/DSM 15641/ 
                 MARCQARGLNPLAGDAYMTVYQGKDGNTSSSVIVSKDYFVRT 
                   
               
               
                   
                   
                   
                 12-3) 
                 ATAQDSFDGMEAGVTVLNGQGQIQKREGCEFFPSLGEKLLGG 
                   
               
               
                   
                   
                   
                   
                 WAKVHVKDREHPSKAAVTMDEYDQHRSLWKSKPATMIRKVA 
                   
               
               
                   
                   
                   
                   
                 IVQALREAYPGQFGGVYDRDEMPPSQEPQQVPVEVYEAPEAVE 
                   
               
               
                   
                   
                   
                   
                 TPDNQNRATEEF 
                   
               
               
                   
               
               
                 SR063 
                 SSAP 
                 ERF 
                 Enterobacteria phage T1 
                 MHLIHQSGEVKMQLSPETNEILPALFNARNKFAKAKKDAKNN 
                 71 
               
               
                   
                   
                   
                 (Bacteriophage T1) 
                 HLKNSVATLDAMMAAVSPALTDNDIMILQSMLDTSTETTFHLE 
                   
               
               
                   
                   
                   
                   
                 TMLIHKSGQWAKFFMMMPIAKRDPQGVGSAMTYARRYSLAA 
                   
               
               
                   
                   
                   
                   
                 ALGISQSDDDAQLAVKSVKDWKKELDACEDIESLKDVWANAY 
                   
               
               
                   
                   
                   
                   
                 RQTDTASKSIIQDHYNALKAKFEIGKARGIRPAQPEQKKQVEAT 
                   
               
               
                   
                   
                   
                   
                 SAKPVQSQSITNFE 
                   
               
               
                   
               
               
                 SR064 
                 SSAP 
                 recT 
                   Acinetobacter  sp SH024 
                 MQDVDPAELANTLVNTVFKKATNDEFLSLLIVANQYKLNPFTK 
                 72 
               
               
                   
                   
                   
                   
                 EIYAFPAKGGGITPVVGIDGWARIINDNPVCDGIQFEQDDESCT 
                   
               
               
                   
                   
                   
                   
                 CKIFRKDRNHPTVVTEVLSECQGNSEPWKKYPKRMERHKALIQ 
                   
               
               
                   
                   
                   
                   
                 CARVAFGFSGIYDEDEARRIDDCHIPTVQTVSSDLPQGYEAYEQ 
                   
               
               
                   
                   
                   
                   
                 QHLDNMRALAMEGTEALQTGYAELPQGDCKKYFWTKHSASL 
                   
               
               
                   
                   
                   
                   
                 KEAAQHADQPQGQVYEHSPA 
                   
               
               
                   
               
               
                 SR065 
                 SSAP 
                 other 
                   Paenibacillus alvei  DSM 29 
                 MQRKKLKRVMKSTNMGEDALLKTFDNFDLSDLDQRVINSYEL 
                 73 
               
               
                   
                   
                   
                   
                 AREYSDGLIYRIKNAKSLKGVPWFGILGTSGSGKTHLVTAAVA 
                   
               
               
                   
                   
                   
                   
                 PLIKYDVYPLFFNWVQSFTEWFSYYNTDESYMVEEIRQRIYNCE 
                   
               
               
                   
                   
                   
                   
                 LLVVDDICKESQKDTWIKEFYGIVDYRYRKQLPIVYTSEYFAEL 
                   
               
               
                   
                   
                   
                   
                 IGFLSKATAGRLFERTVSPKGKKFLGEMLLNDGEDPLALDYRF 
                   
               
               
                   
                   
                   
                   
                 KELFR 
                   
               
               
                   
               
               
                 SR066 
                 SSAP 
                 sak4 
                   Lactobacillus  phage Lc-Nu 
                 MQPIKHASAIDRTKNWRVLIYGKPGVGKTSAIRNLNGKTLVLD 
                 74 
               
               
                   
                   
                   
                   
                 LDDSSKVLSGAPNIDVQPFDRSKPSEEWKEFLKNLAERVSGYD 
                   
               
               
                   
                   
                   
                   
                 NLVIDNVSAFEKDWFVERGRASKNGIGNEIQDYGQWANYFARI 
                   
               
               
                   
                   
                   
                   
                 MTMIFMDAPVNVLVTAWENTRDITSETGQSFSQYAPAIRDSVR 
                   
               
               
                   
                   
                   
                   
                 DGLLGLTDVVGRVVVNPKTDGRGVILEGTDAIFAKNRLDNRK 
                   
               
               
                   
                   
                   
                   
                 LVPINELFKFGNQEKSVKQED 
                   
               
               
                   
               
               
                 SR067 
                 SSAP 
                 sak4 
                   Pseudomonas  phage vB_Pae- 
                 MQMSQLKPASQLARRYGVKSVVFGAPGSGKTPLINTAPRPVLL 
                 75 
               
               
                   
                   
                   
                 Kakheti25 
                 VTEPGMLSMRGSNVPAWEAYTPALMVEFFEWFMKSREAANF 
                   
               
               
                   
                   
                   
                   
                 DTLGIDSISNIAEIILADELGKVKHGMKAYGNMSERVMKIANDL 
                   
               
               
                   
                   
                   
                   
                 YYMPQKHIVMIAKQALVENGRQTILQNGEVTYEPIMQKRPFFP 
                   
               
               
                   
                   
                   
                   
                 GKDLNVKVPHLFDNVMHLGEASVPGMPKPVRALRTKEIPEVF 
                   
               
               
                   
                   
                   
                   
                 ARDRLGNLNELEQPDLTALFAKAMQ 
                   
               
               
                   
               
               
                 SR068 
                 SSAP 
                 ERF 
                   Escherichia  phage Rtp 
                 MQISELCKSILKALHTAKSLFAKAEKSKQNSHLKNKYATLEDV 
                 76 
               
               
                   
                   
                   
                   
                 LAAVEPGLYECGLVMFQSVLDDEQTNRMKVETKLFHVESGEW 
                   
               
               
                   
                   
                   
                   
                 VSFLMIVPISKNDAQGYGSALTYARRYGITAALGLSQADDDGN 
                   
               
               
                   
                   
                   
                   
                 LAAKGVKDFKRELEKCNTLDELRNVWKEAKQSLDAAGWKVF 
                   
               
               
                   
                   
                   
                   
                 EPHIIERKAELEANAMTGFNPATPKKVAEKSSPEEKIKVESQQID 
                   
               
               
                   
                   
                   
                   
                 TF 
                   
               
               
                   
               
               
                 SR069 
                 SSAP 
                 recT 
                   Streptomyces  phage VWB 
                 MIQTIAFDVGETLVSDDRYWASWADWLGAPRHTVSALVGAV 
                 77 
               
               
                   
                   
                   
                   
                 VAQGRDNADALRLVRPGLDVAAEYAAREAAGRGEHLDDTDL 
                   
               
               
                   
                   
                   
                   
                 YDDVRPALAALQALGVRVIIAGNQTIRAGELLRGLDLPVDVIAT 
                   
               
               
                   
                   
                   
                   
                 SGEWGCAKPTREFFDRVVDVAGTPRQSILYVGDHPANDTIPAA 
                   
               
               
                   
                   
                   
                   
                 AAGLRTAHLRRGPWGHLWADDPQVRDTADWVIGSLHDLIDIV 
                   
               
               
                   
                   
                   
                   
                 AGT 
                   
               
               
                   
               
               
                 SR070 
                 SSAP 
                 rad52 
                   Paenibacillus alvei  DSM 29 
                 MINGKTVEQVMAELAEPFPPEDIEWRVGSTNGGKTKGIALAYV 
                 78 
               
               
                   
                   
                   
                   
                 TNRAIMNRLDSVVGAFNWQNNYREWKGHSQLCGIGIRFGEDW 
                   
               
               
                   
                   
                   
                   
                 IWKWDGADDSQTEAVKGGLSDSMKRAGYQWGIGRYLYKLEN 
                   
               
               
                   
                   
                   
                   
                 VWVPIEPIGKSYRLAQTPKLPQWALPTGYTGQQTNTGNKTQRS 
                   
               
               
                   
                   
                   
                   
                 TQKTQGKQENNSPSNNDGGSQQSSNDTGTQKRQMTEKQKQR 
                   
               
               
                   
                   
                   
                   
                 WEVVRLLKAGGLDDAQAQAWIDKQKEQKRDYKTMLDICQRA 
                   
               
               
                   
                   
                   
                   
                 SKR 
                   
               
               
                   
               
               
                 SR071 
                 SSAP 
                 recT 
                 
                   Listeria phage B054, Listeria 
                 
                 MMAKENYSDPNGKLLNSITTFEVNGEEVKLSGNIIRDYLVSGN 
                 79 
               
               
                   
                   
                   
                 monocytogenes 
                 AEVTDQEIIMFLQLCKYQKLNPFLNEAYLVKFKNTKGPDKPAQ 
                   
               
               
                   
                   
                   
                   
                 IIVSKEAFMKRAETHEQYDGFEAGVIVERGGEIIELEGAVSLASD 
                   
               
               
                   
                   
                   
                   
                 KLLGGWAKVFRKDRNRPVSVRISEKEFNKRQSTWNTMPLTMM 
                   
               
               
                   
                   
                   
                   
                 RKTAVVNAMREAFPDNLGAMYTEEEQGSLQNTETSVQQEIKQ 
                   
               
               
                   
                   
                   
                   
                 NANAEVLDIPSQQNEVPDFKEVREPEHVEMPPIYGEQQSTPPAR 
                   
               
               
                   
                   
                   
                   
                 PY 
                   
               
               
                   
               
               
                 SR072 
                 SSAP 
                 ERF 
                   Mycobacterium  phage Wildcat 
                 MMRSSDNCADLLTALAKARTEIGPVEKSAQNPFFNSSYMTLDD 
                 80 
               
               
                   
                   
                   
                   
                 IMDAVQPVLAKNGLSVSQWPDQSVNGEPALTTLLFHEPSGQYV 
                   
               
               
                   
                   
                   
                   
                 QATATLVLGKKDPQAEGSAITYLRRYAYVSILGIKGVEPDDDG 
                   
               
               
                   
                   
                   
                   
                 NYASQSDKKVTRTGKVADESNASAEVTRLRKELEVAAKAAGL 
                   
               
               
                   
                   
                   
                   
                 TSAALAKGYHDRYGADYKRDNDETHLAAALTEMQLRVKSE 
                   
               
               
                   
               
               
                 SR073 
                 SSAP 
                 sak 
                   Rhodothermus  phage RM378 
                 MMNPEMKEILKKLMKPFHPDRHSYRVTGTFRTREGRNMGVV 
                 81 
               
               
                   
                   
                   
                   
                 AFYISSRDVMDRLDAVVGPENWRDEYEVPAPGVMKCVLYLRI 
                   
               
               
                   
                   
                   
                   
                 GGEWVGKSDVGTGNIENPESGWKGAASDALKRAAVKWGIGR 
                   
               
               
                   
                   
                   
                   
                 YLYALPKCYVEVDDRKRIVNEEAVKSFLHKHVTELLKNYQ 
                   
               
               
                   
               
               
                 SR074 
                 SSAP 
                 other 
                 
                   Paenibacillus terrae 
                 
                 MIQGTLFDNEHDRPLPNRVRPQSLEDFIGQKHLLGPGKVLQDM 
                 82 
               
               
                   
                   
                   
                 (strain HPL-003) 
                 IKNDQVSSMIFWGPPGVGKTTLAKIIANQTKSKFIDFSAVTSGIK 
                   
               
               
                   
                   
                   
                   
                 DIRNVMKEAEGNRQLGEKTLLFIDEIHRFNKAQQDAFLPYVEKG 
                   
               
               
                   
                   
                   
                   
                 SIILIGATTENPSFEVNSALLSRSKVFVLHQLSSEDIVELLEKAI 
                   
               
               
                   
                   
                   
                   
                 LDPKGYGDQKIGFEDGVLSAIAEYSNGDARVALNTLEMAVLN 
                   
               
               
                   
                   
                   
                   
                 GEKQGEAIEISKEGLLQIIHRKSLLYDKDGEEHYNIISALHKSMR 
                   
               
               
                   
                   
                   
                   
                 NSDVNASIYWLSRMLESGEDPLFIARRLVRFASEDVGLADNRA 
                   
               
               
                   
                   
                   
                   
                 LEITVSVFQACQFIGMPECDVHLTQAVIYLTLAPKSNSAYLAYR 
                   
               
               
                   
                   
                   
                   
                 YAKKDALHSMSEPVPLQLRNAPTKLMKELNYGKGYQYAHDT 
                   
               
               
                   
                   
                   
                   
                 EEKLTRMQTMPDSLIGREYYHPTTQGSESKIKQRMEEIAAWYK 
                   
               
               
                   
                   
                   
                   
                 QNNNQK 
                   
               
               
                   
               
               
                 SR075 
                 SSAP 
                 sak4 
                   Listonella  phage phiHSIC 
                 MSVLSSITKPVDRPVIMTVLGEAGLGKTSLAATFPKPIFIRTEDG 
                 83 
               
               
                   
                   
                   
                   
                 LQAIPEASRPDAFPMADTVESLMEQLGALVHEEHDYKTLVIDS 
                   
               
               
                   
                   
                   
                   
                 VTQLETMFTQHVIDNDPKKPKSIQQANGGYGSGLSAVAALHG 
                   
               
               
                   
                   
                   
                   
                 RVRKAAKMLNDKGMHIVFIAHADTETIELPDQDPYMRYSLRL 
                   
               
               
                   
                   
                   
                   
                 GKKSMSHYVDNVDLVGLVKLQMFLKGDDDKRKKAISTGQRVI 
                   
               
               
                   
                   
                   
                   
                 TATTSASSVTKNRYGITQDLPCELGVNPFINHIPSLTE 
                   
               
               
                   
               
               
                 SR076 
                 SSAP 
                 sak4 
                   Staphylococcus  phage Pv1108 
                 MSEEQDILQELGIEEINEDTQNYYSIMVYGKSGTGKTTLATREN 
                 84 
               
               
                   
                   
                   
                   
                 NAFIIDIHEDGTQVTRQGFVKRVDNYIAFRNTITSIESIVNTARQ 
                   
               
               
                   
                   
                   
                   
                 RGKLLDVVVIETAQKLRDITLTHVMNTHQVKKARIQDYGETSK 
                   
               
               
                   
                   
                   
                   
                 LIVNSIRHLLKVKDKLGFHVVLTGHEGLNSEDKDENGKIINPRIS 
                   
               
               
                   
                   
                   
                   
                 IEVQPAIHNNLVTQFDIIGHTFIEDHTDENGNATHNYVESVEPSN 
                   
               
               
                   
                   
                   
                   
                 LYTTKVRHNPQITINNPGIKNASISKIIDMAQNGN 
                   
               
               
                   
               
               
                 SR077 
                 SSAP 
                 sak 
                   Mycobacterium  phage Hamulus, 
                 MSEPDVEGLAKLREPFPPNQIGKLPKGGITLDFLGHGYLTARFL 
                 85 
               
               
                   
                   
                   
                   Mycobacterium  phage Dante, 
                 DVDPLWTWEPFAVGDNGLPLLDEHGGLWIRLTLCGVTRIGYG 
                   
               
               
                   
                   
                   
                   Mycobacterium  phage 
                 DAGGKKGPNAVKEAIGDALRNAGMRFGAALDLWCKGDPDAP 
                   
               
               
                   
                   
                   
                 Ardmore,  Mycobacterium  phage 
                 APPDPAVAERNALLHELGDACAALTLDEKTVAAQFYGKYKVT 
                   
               
               
                   
                   
                   
                 Llij,  Mycobacterium  phage Drago, 
                 ARNAKPAQLREFIDDLMENGAPA 
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Phatniss, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Spartacus, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Boomer, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage SiSi, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage PMC, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Ovechkin, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Ramsey, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Fruitloop, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage SG4 
                   
                   
               
               
                   
               
               
                 
                   SR078 
                 
                 SSAP 
                 recT 
                   Bacillus subtilis  subsp 
                 MSEQNELMTKSVEFSVHGEPVKLTGKTVKNFLVRGNSDVTDQ 
                 86 
               
               
                   
                   
                   
                   spizizenii  (strain TU-B-10), 
                 EAAMFINLCKYQKLNPFLNEAYLVKFKGSPAQMIVGKEAFMK 
                   
               
               
                   
                   
                   
                   Jeotgalibacillus   marinus   
                 RAENNEQFQGFKAGIIVEREGKMVDLEGAIKLSNDKLIGGWAE 
                   
               
               
                   
                   
                   
                   
                 VYRADRQQPISVRISLEEFSKGQSTWKSMPLNMIRKTAIVNALR 
                   
               
               
                   
                   
                   
                   
                 EAFPNSLGNLYTEEEEQANDDILAEDRVRREVNENANTEIIDVN 
                   
               
               
                   
                   
                   
                   
                 PILEPEPETTQAGQPEPQPIKRESQDKPSIFESGPDF 
                   
               
               
                   
               
               
                 SR079 
                 SSAP 
                 recT 
                   Paenibacillus lactis  154 
                 MSSQQLALVKRDTVDVVADKVRQFQERGEIHEPANYSPENAM 
                 87 
               
               
                   
                   
                   
                   
                 KSAWLLLQTVQTKDYKPALEVCSRDSIANALLDMVVQGLNPA 
                   
               
               
                   
                   
                   
                   
                 KKQGYFIVYGKTLTFQRSYFGTMAVTKRVTGAEDIDAQIIYEG 
                   
               
               
                   
                   
                   
                   
                 DEFEFEIVRGRKKITKHKPSFSNMDDSKIAGAYCTIYWPDGREY 
                   
               
               
                   
                   
                   
                   
                 TEVMTMKEIRQAWKKSKQNPDKENSTHNEFPGEMAKRTVINR 
                   
               
               
                   
                   
                   
                   
                 TCKTYMNTSDDGSLIMKHFKRQDEVLAEAEVEAEIAANANGE 
                   
               
               
                   
                   
                   
                   
                 VIDITPPATTEAEPEPEQPKETTRSSKSNVELAGQGEMDFEIHPD 
                   
               
               
                   
                   
                   
                   
                 DIPPFGTEGPDE 
                   
               
               
                   
               
               
                 SR080 
                 SSAP 
                 recT 
                   Pediococcus acidilactici  DSM 
                 MSNELMTKAVTYEVNNEEVKLSGQIVKQYLTSGQAVTDQEVT 
                 88 
               
               
                   
                   
                   
                 20284 
                 MFIQLCRYQHLNPFLNEAYLVKENGKPAQIITSKEAFMKRAESN 
                   
               
               
                   
                   
                   
                   
                 PNYAGLKAGCIVERNGELIYTEGAFTLKTDNILGAWADVIRKD 
                   
               
               
                   
                   
                   
                   
                 RREPTHVEISMDEFSKSQATWKSMPATMIRKTAIVNALREAFP 
                   
               
               
                   
                   
                   
                   
                 QDLGALYTEDDKNPNEATQTTYKQEPEVNTTKTADVLAKKFS 
                   
               
               
                   
                   
                   
                   
                 GAPQIKSVENVQESEEESNNASNHGEATEPVNNVEEPTATAEV 
                   
               
               
                   
                   
                   
                   
                 EQGQLL 
                   
               
               
                   
               
               
                 SR081 
                 SSAP 
                 ERF 
                   Enterobacteria  phage HK022 
                 MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKP 
                 89 
               
               
                   
                   
                   
                 (Bacteriophage HK022) 
                 LLNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREE 
                   
               
               
                   
                   
                   
                   
                 ESKKGMDSAQVTGATSSYARKYCLNGLFGIDDAKDADTDEHK 
                   
               
               
                   
                   
                   
                   
                 HQQNAAAKQSNPSPTPEQVLKAFTDAAMQKNTVEELKQAFAK 
                   
               
               
                   
                   
                   
                   
                 AWKMLEGTPEQHKAQDVYNIRRDELEGAAA 
                   
               
               
                   
               
               
                 SR082 
                 SSAP 
                 rad52 
                   Homo sapiens  (Human) 
                 MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKAL 
                 90 
               
               
                   
                   
                   
                   
                 RQRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWA 
                   
               
               
                   
                   
                   
                   
                 HSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHEDVGYG 
                   
               
               
                   
                   
                   
                   
                 VSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKD 
                   
               
               
                   
                   
                   
                   
                 YLRSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARYNSCRPNMA 
                   
               
               
                   
                   
                   
                   
                 LGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVESEATHQR 
                   
               
               
                   
                   
                   
                   
                 KLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHST 
                   
               
               
                   
                   
                   
                   
                 PVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVV 
                   
               
               
                   
                   
                   
                   
                 KPSSRADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGS 
                   
               
               
                   
                   
                   
                   
                 WDLQTYSADQRTTGNWESHRKSQDMKKRKYDPS 
                   
               
               
                   
               
               
                 SR083 
                 SSAP 
                 recT 
                   Burkholderia cenocepacia  (strain 
                 MSDVITTNQDTAPGAFDLSPRSLEQAMQLANILADSSIVPKDFI 
                 91 
               
               
                   
                   
                   
                 ATCC BAA-245/DSM 16553/ 
                 GKPGNVLVAIQWGMELGLKPMQAMQNIAVINGRPSLWGDAL 
                   
               
               
                   
                   
                   
                 LMG 16656/NCTC 13227/ 
                 LALVLASPVCEYVHEWEENGTAFIKVKRRGKPEDVQSFGDED 
                   
               
               
                   
                   
                   
                 J2315/CF5610) ( Burkholderia   
                 AKKAGLIGKQGPWAQYPQRMKKMRARAFALRDNFADVLKGI 
                   
               
               
                   
                   
                   
                   cepacia  (strain 
                 AVAEEVMDIEPVERDITPRATAAQIAHSAADSSRPARTERHDEI 
                   
               
               
                   
                   
                   
                 J2315)),  Burkholderia cenocepacia   
                 VKKLEDVARNLGFEPFKEEWTKLSRDDRAALGLRERDRIAAIA 
                   
               
               
                   
                   
                   
                 BC7 
                 GAPVVHQQTDGAPQDDGHGAGQREPGGDDE 
                   
               
               
                   
               
               
                 SR084 
                 SSAP 
                 recT 
                   Photorhabdus luminescens  subsp 
                 MSTAVQKVYEIINPLKTEFEQICSEPGIVFKRESEFAMQIFANND 
                 92 
               
               
                   
                   
                   
                   laumondii  (strain DSM 15139/ 
                 FLATTALNNPVSARSAVMNIAAIGISLNPAQKLAYLVPRNKSVC 
                   
               
               
                   
                   
                   
                 CIP105565/TT01) 
                 LDISYMGLMHIAQQSGAIKWCQSAVVRKNDIFKRTSIDTAPIHE 
                   
               
               
                   
                   
                   
                   
                 YNEFDTAQSRGDIVGAYTVIKTDDCDYITHTMRASAIFDIRDRS 
                   
               
               
                   
                   
                   
                   
                 SAWIAYKTKGKSCPWVTDEEQMILKTVVKQAAKYWPRRERL 
                   
               
               
                   
                   
                   
                   
                 DKAIDYVNTEAGEGIDFSKGQNSVIDVTPAADSTIESITNLLTTM 
                   
               
               
                   
                   
                   
                   
                 NKTWDDDLLPLCSKIFRRQILSPAELTESEAIKASDFLRKKAS 
                   
               
               
                   
               
               
                 SR085 
                 SSAP 
                 recT 
                 
                   Bacillus thuringiensis 
                 
                 MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDA 
                 93 
               
               
                   
                   
                   
                 Sbt003,  Escherichia coli \′BL21- 
                 QFIALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRII 
                   
               
               
                   
                   
                   
                 Gold(DE3)pLysS 
                 NENQQFDGMDFEQDNESCTCRIYRKDRNHPICVTEWMDECRR 
                   
               
               
                   
                   
                   
                 AG\′, Enterobacteria phage 
                 EPFKTREGREITGPWQSHPKRMLRHKAMIQCARLAFGFAGIYD 
                   
               
               
                   
                   
                   
                 HK630, Enterobacteria phage 
                 KDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKT 
                   
               
               
                   
                   
                   
                 lambda (Bacteriophage 
                 WDDDLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQK 
                   
               
               
                   
                   
                   
                 lambda),  Escherichia coli   
                 VAA 
                   
               
               
                   
                   
                   
                 TA280,  Escherichia coli  1-176- 
                   
                   
               
               
                   
                   
                   
                 05_S3_C2,  Escherichia coli  40967 
                   
                   
               
               
                   
               
               
                 SR086 
                 SSAP 
                 recT 
                   Listeria  phage A118 
                 MSTNDELKNKLANKQNGGQVASAQSLGLKGLLEAPTMRKKFE 
                 94 
               
               
                   
                   
                   
                 (Bacteriophage A118) 
                 SVLDKKAPQFLTSLLNLYNGDDYLQKTDPMTVVTSAMVAATL 
                   
               
               
                   
                   
                   
                   
                 DLPIDKNLGYAWIVPYKGRAQFQLGYKGYIQLALRTGQYKSIN 
                   
               
               
                   
                   
                   
                   
                 VIEVRDGELLKWNRLTEEIELDLDNNTSEKVIGYCGYFQLINGF 
                   
               
               
                   
                   
                   
                   
                 EKTVYWTRKEIEAHKKKFSKSDFGWKKDYDAMAKKTVLRNM 
                   
               
               
                   
                   
                   
                   
                 LSKWGILSIDMQTAVTEDEAEPRERKDVTEDESIPDIIDAPITP 
                   
               
               
                   
                   
                   
                   
                 SDTLEAGSEVQGSMI 
                   
               
               
                   
               
               
                 SR087 
                 SSAP 
                 ERF 
                   Streptococcus pyogenes  serotype 
                 MRKSESITEYAKAFCKAQLEVKQPLKDKDNPFFKSKYVPLENV 
                 95 
               
               
                   
                   
                   
                 M28 (strain 
                 TEAITKAFANNGISFSQDPTTNAENGYIDVATLVMHTSGEWVE 
                   
               
               
                   
                   
                   
                 MGAS6180),  Streptococcus   
                 YGPLSVKPTKNDVQGAGSAITYAKRYALSAIFGITSDQDDDGN 
                   
               
               
                   
                   
                   
                   pyogenes , Temperate phage 
                 EASKPNKTNQSQKPTNKTSKGASFQTPKISNIQVETYKSDLSDI 
                   
               
               
                   
                   
                   
                 phiNIH11,  Streptococcus pyogenes   
                 AKATNQNVEELTKWLTDTLKVKTLEDLRTEQIVSTDNLINKLK 
                   
               
               
                   
                   
                   
                 serotype M2 (strain 
                 KKAEQKND 
                   
               
               
                   
                   
                   
                 MGAS10270),  Streptococcus   
                   
                   
               
               
                   
                   
                   
                   pyogenes  serotype M3 (strain 
                   
                   
               
               
                   
                   
                   
                 ATCC BAA-595/ 
                   
                   
               
               
                   
                   
                   
                 MGAS315),  Streptococcus   
                   
                   
               
               
                   
                   
                   
                   pyogenes  STAB902 
                   
                   
               
               
                   
               
               
                 SR088 
                 SSAP 
                 sak4 
                   Staphylococcus phage  phill 
                 MTEKTNQDVDILTQLGVKDISKQNANKFYKFAIYGKFGTGKTT 
                 96 
               
               
                   
                   
                   
                 (Bacteriophage phi- 
                 FLTKDNNALVLDINEDGTTVTEDGAVVQIKNYKHFSAVIKMLP 
                   
               
               
                   
                   
                   
                 11),  Staphylococcus  phage 
                 KIIEQLRENGKQIDVVVIETIQKLRDITMDDIMDGKSKKPTFND 
                   
               
               
                   
                   
                   
                 80,  Staphylococcus  phage 
                 WGECATRIVSIYRYISKLQEHYQFHLAISGHEGINKDKDDEGSTI 
                   
               
               
                   
                   
                   
                 52A,  Staphylococcus aureus   
                 NPTITIEAQDQIKKAVISQSDVLARMTIEEHEQDGEKTYQYVLN 
                   
               
               
                   
                   
                   
                 (strain NCTC 8325) 
                 AEPSNLFETKIRHSSNIKINNKRFINPSINDVVQAIRNGN 
                   
               
               
                   
               
               
                 SR089 
                 SSAP 
                 rad52 
                   Pseudomonas aeruginosa , 
                 ATGACTAACATGAATTCAAATATGGCTATCTGGGATCAAGT 
                 97 
               
               
                   
                   
                   
                 
                   Pseudomonas aeruginosa 
                 
                 GAAAGAGACAGATACCAGATATACCAGACAAGCTAAGCTCA 
                   
               
               
                   
                   
                   
                 DHS0,  Pseudomonas  phage 
                 ATGGCCAGGACATGACATCCATCAACGGACTCTATATAGTT 
                   
               
               
                   
                   
                   
                 LKA5,  Pseudomonas  phage F116 
                 CGCCGCGCTACAGAATTATTTGGTCCAGTTGGCAAAGGTTG 
                   
               
               
                   
                   
                   
                   
                 GGGTTGGAAAGTCCTCGTAGAACGTTTTGACGAAGGGGCCC 
                   
               
               
                   
                   
                   
                   
                 CGCATTTAGACAAGAATGGTGCGGTTATTTGTCACGATAAA 
                   
               
               
                   
                   
                   
                   
                 ACACATACCTTGTATATAGAATTGTGGTACCGTCATGATGGA 
                   
               
               
                   
                   
                   
                   
                 ACAATTAACCATGCTAGGCAGTATGGGCATACCCCGTACGT 
                   
               
               
                   
                   
                   
                   
                 CTATAAAACTGAGTGGGGTTTCAAAACGGATCACGATTATG 
                   
               
               
                   
                   
                   
                   
                 GGAAAAAATCGCTCACAGATGCGATTAAAAAGTGCCTGAGT 
                   
               
               
                   
                   
                   
                   
                 TTGCTCGGTTTTTCTGCGGATATCCACATGGGTATGTTCGAC 
                   
               
               
                   
                   
                   
                   
                 GATACAACGTATGTTGAGGGTCTGAAATTAAAAGAGAGATT 
                   
               
               
                   
                   
                   
                   
                 AGCTGATGCTGGTGATCCGGAGACTGCTTTAGATGAAGCCA 
                   
               
               
                   
                   
                   
                   
                 AAGACGAGTTTAAAACTTGGCTTCGTGCCCAGTTAGATGCC 
                   
               
               
                   
                   
                   
                   
                 ATTGCTGCTGCTCCGAATAGCCGTGCTTTAGAACTAATGCGC 
                   
               
               
                   
                   
                   
                   
                 AAACAGGTGGCCGAAAAAGCTAGAGCCAAAGCCCCTGTAGT 
                   
               
               
                   
                   
                   
                   
                 TAATTTCAATCCGAGCGAAATTGAGCTGAGAGTGAATGAGG 
                   
               
               
                   
                   
                   
                   
                 CGGCGGATGAGAGGCTAAGACAGCTATCTCCCGCCCCGACG 
                   
               
               
                   
                   
                   
                   
                 AGCCCGGAAGAGTAA 
                   
               
               
                   
               
               
                 SR090 
                 SSAP 
                 sak 
                   Staphylococcus  phage CNPH82 
                 MTEETLFNQLNQKDVNDHVEKKNGLTYLAWSVAHQELKKIDS 
                 98 
               
               
                   
                   
                   
                   
                 NYSIKTHEFVHPDVPQDNYFVPYLATPEGYFVQVSVTVKGQTE 
                   
               
               
                   
                   
                   
                   
                 TEWLPVLDFRNKSLAKGSATTFDINKAQKRCFVKAAALHGLG 
                   
               
               
                   
                   
                   
                   
                 LYIYNGEEVPSANDNDITELEERINQFVTLSQEKGRDATLDKTM 
                   
               
               
                   
                   
                   
                   
                 RWLGIQNINKVTKKDIANAHQKLDAGLKQLDKENSND 
                   
               
               
                   
               
               
                 SR091 
                 SSAP 
                 gp2.5 
                 
                   Streptococcus pyogenes 
                 
                 MTTTPNTTKVVTGKVRLSYVALLEPKAFEGQEAKYSTVILIPKT 
                 99 
               
               
                   
                   
                   
                 STAB902,  Streptococcus   
                 DKVTIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDE 
                   
               
               
                   
                   
                   
                   pyogenes ,  Streptococcus   
                 EMDTEEHPEYTGHMFMSVSSKTKPQIIDKYKNFVDSAEEVYSG 
                   
               
               
                   
                   
                   
                   pyogenes  serotype M3 (strain 
                 VYARVSLNAYAYNTAGNKGISCGLNNVQIVAKGDYLGGRSSA 
                   
               
               
                   
                   
                   
                 ATCC BAA-595/MGAS315) 
                 DADFDEWNEEEDEDDIL 
                   
               
               
                   
               
               
                 SR092 
                 SSAP 
                 other 
                 
                   Paenibacillus curdlanolyticus 
                 
                 MTNMQHMMKQVKKMQEQMLKAQEELGTKTIEGSAGGGVVN 
                 100 
               
               
                   
                   
                   
                 YK9 
                 VKVNGHKQVLSITIKPEVVDPEDIEMLQDLVITAVNDALAKAE 
                   
               
               
                   
                   
                   
                   
                 EMANEDMGKYTGGMKIPGLF 
                   
               
               
                   
               
               
                 SR093 
                 SSAP 
                 recT 
                   Streptococcus  phage 
                 MTNNQLATQTKRNITTDPSLLTGADIKKYFDPQNLLTEKQVGQ 
                 101 
               
               
                   
                   
                   
                 V22,  Streptococcus pneumoniae   
                 ALALCKGRNLNPFANEVYIVAYTNRNGGKEFSLIVSKEAFLKR 
                   
               
               
                   
                   
                   
                   
                 AAQCKDYEGFEAGVVVVDSEGVMHERKGAIMLPEDTLIGGW 
                   
               
               
                   
                   
                   
                   
                 ARVHRKNFKVPVEIFVSREEYDKKQSTWNTMPATMIRKVALV 
                   
               
               
                   
                   
                   
                   
                 NALREAFPEDLGNMYTEDDGGETFDRIKDVTPQESREDVVARK 
                   
               
               
                   
                   
                   
                   
                 MAEIEQFNKEQEANHADPEPAQNEDPIQGELLDGELEY 
                   
               
               
                   
               
               
                 SR094 
                 SSAP 
                 recT 
                 Cyanophage PSS2 
                 MTEQHAQRWDERTKKIVFATVAKDVRSEADKAHFEHLCTSKG 
                 102 
               
               
                   
                   
                   
                   
                 LDPLAKEIWCVARGGKPTFMLSIDGFLKLANSTGMLDGIDIEFF 
                   
               
               
                   
                   
                   
                   
                 DADGKGSEVWVSSKPPAACVARAYRKNCSRPFSASCRFDAYA 
                   
               
               
                   
                   
                   
                   
                 QNSPLWKKLPEVMLSKVATTLALRRGFSDVLSGLHSPEEMDQ 
                   
               
               
                   
                   
                   
                   
                 AGMAPAEALAPSAPAAPAPVVNPPAEKPRKRMAPVEPVHEVID 
                   
               
               
                   
                   
                   
                   
                 RPTDDLHHGGVTEVQKNVAPKAVAVMEKPVEEGLPARDDLK 
                   
               
               
                   
                   
                   
                   
                 GAISKLYEVARAKGLTVKGWESLGLQMGGLTPGSAAKFLTPM 
                   
               
               
                   
                   
                   
                   
                 SSISEEKVGYLNSGKSTTGEALR 
                   
               
               
                   
               
               
                 SR095 
                 SSAP 
                 recT 
                   Vibrio cholerae  1587 
                 MTQVIPFEQQYPLVAQRGIDEATWSALQNSVYPGAKPESILMV 
                 103 
               
               
                   
                   
                   
                   
                 VDYCRARSLDPILKPVHIVPMSVKNSQTNQNEWRDVVMPGIG 
                   
               
               
                   
                   
                   
                   
                 MYRIQADRSKTYAGSTEPEFGPPITMEFHGEGNAKETITEPEWC 
                   
               
               
                   
                   
                   
                   
                 KITVYKLINGSPVAFSAKEMWLENYATAGRNSQIPNAMWKKR 
                   
               
               
                   
                   
                   
                   
                 QYAQLAKCTEAQALRKAWPEIGQQATAEEMEGKELIIEHDITP 
                   
               
               
                   
                   
                   
                   
                 AKKSAPQIQQYPQDDFDKNFPAWEKKIKLGTNTPERIIAMVQS 
                   
               
               
                   
                   
                   
                   
                 RGQLTDEMKQRLFDASAINGELSHANSN 
                   
               
               
                   
               
               
                 SR096 
                 SSAP 
                 recT 
                 
                   Haemophilus 
                 
                 MTTALNTLTQKLAERFEMGSSENLPQTLMATAFKGQNVTPDQ 
                 104 
               
               
                   
                   
                   
                   paraphrohaemolyticus  HK411 
                 MVALLVVANQYELNPWTNEIYAFPNKEGIIPIVGVDGWVAIIN 
                   
               
               
                   
                   
                   
                   
                 KHPQFDGIEFEQDAESCTCRIYRKDRQRPTVVTEYLDECKRETD 
                   
               
               
                   
                   
                   
                   
                 PWRKYPKRMLRHKAAIQCARVAFSLSGIYEPDEAERIQESDKTS 
                   
               
               
                   
                   
                   
                   
                 QNITPEKSATTGNPTHPEFEALVFTLEEEAKKGTSHLETYWKTT 
                   
               
               
                   
                   
                   
                   
                 LTKEQRQIVGVNEITRIKEKAKQYDNIQEAEYEEA 
                   
               
               
                   
               
               
                 SR097 
                 SSAP 
                 recT 
                 
                   Bartonella schoenbuchensis 
                 
                 MTTSIVATVAQKYGLSEQEFCKKIIKNCINFNISKEDFEDFVYL 
                 105 
               
               
                   
                   
                   
                 (strain DSM 13525/NCTC 13165/R1) 
                 ADGYKLNPLRKEIYAVPKRGGGIDAVVGIKGWYKLIRSQDDYD 
                   
               
               
                   
                   
                   
                   
                 GIDIIYQHDNNGKLHAAQCIIYLKSKKYPIKFTEFLEECRRDTE 
                   
               
               
                   
                   
                   
                   
                 HWRKSSSRMLCYKAITQCARLAFGFDDIYDEDEVDCIDEAFISE 
                   
               
               
                   
                   
                   
                   
                 VNHNPQNERVSDEVLAQIRELMEQTKTEEKKVLSFAKVASLTEM 
                   
               
               
                   
                   
                   
                   
                 SHETGQIILEGLKERQRFQMEEEQQTLPSPKQPHTPTQQDLPGV 
                   
               
               
                   
               
               
                 SR098 
                 SSAP 
                 ERF 
                   Phormidium  phage Pf-WMP3 
                 MTNNITPDFIKAFVKVQSELPAIPKSSTNPHFKSKFASLDTFNE 
                 106 
               
               
                   
                   
                   
                   
                 LVLPVLHTYGFTLMQPCSVENDNAVIDTYLMHESGGYVVSRYLI 
                   
               
               
                   
                   
                   
                   
                 GFDDNPQKQGARVTYGRRYAAFAILGVVGDEDDDGNSAVGLS 
                   
               
               
                   
                   
                   
                   
                 GTKQTSAEPVATLKRSGGERKLQ 
                   
               
               
                   
               
               
                 SR099 
                 SSAP 
                 recT 
                   Simkania negevensis  (strain 
                 MTVQLVQPRNSDEYDFDQTKLDLIKRTICKGATNDELQLFIHA 
                 107 
               
               
                   
                   
                   
                 ATCC VR-1471/Z) 
                 CKRTGLDPFMRQIFAVKRWDSSTKKEIMTIQTGIDGYRLIADRT 
                   
               
               
                   
                   
                   
                   
                 GKYAPGKDTEFGYDNKGNIRWAKAYIKKMTPDGQWHEISAIA 
                   
               
               
                   
                   
                   
                   
                 FWEEYVQTTREGKSTLFWLKKSHIMLSKCTEALAERKTFPAER 
                   
               
               
                   
                   
                   
                   
                 SGIYTKEEMAQEFSPLEEHLVERIAASRNDQGRS 
                   
               
               
                   
               
               
                 SR100 
                 SSAP 
                 sak 
                   Lactobacillus  phage 
                 MTDKKKSVYETLAKVDVKPLLEKKGNLNYLSWAKAWGLVKS 
                 108 
               
               
                   
                   
                   
                 KC5a,  Lactobacillus   
                 LYPDATYQIKEEPEYVLTKESWLATGRNVDYRQTIAGTEVEVT 
                   
               
               
                   
                   
                   
                 phage phi jlb1 
                 VTIEDQSYSSKLYVMDYRNKVIAKPTYFEINKTQMRCLVKALA 
                   
               
               
                   
                   
                   
                   
                 FAGLGLDVYAGEDLPEQPQRKQTVPKPAQKPARRQATLSKPK 
                   
               
               
                   
                   
                   
                   
                 KMTKDQLYGYQADYNGEKKFLVTIYTECDKQKKMGLDAKTS 
                   
               
               
                   
                   
                   
                   
                 VPIEWWHEKLKENSADGEAVRQFTEMAIAANKKKQANGIPDY 
                   
               
               
                   
                   
                   
                   
                 AKDPEVEKAIEDAIS 
                   
               
               
                   
               
               
                 SR101 
                 SSAP 
                 rad52 
                   Thermus  phage phiYS40 
                 MTEKEIRIELMRPFPDQAILFRVDKKLKNGSYLIVPYLDVRVII 
                 109 
               
               
                   
                   
                   
                   
                 HRLNTMIPGDWELKTEITPITVETTDKSGFLIVGHMAKAELTIM 
                   
               
               
                   
                   
                   
                   
                 GKTMTGTGSSYLVFDNDLEKLKKQFIKADPKSAETDAIRRAAAN 
                   
               
               
                   
                   
                   
                   
                 HSIGLYIWFFKNQIFATEEELKNRNSKPIQEALANERIIGDKLY 
                   
               
               
                   
                   
                   
                   
                 RQAKEYALGKRGGAE 
                   
               
               
                   
               
               
                 SR102 
                 SSAP 
                 recT 
                 
                   Streptococcus gallolyticus 
                 
                 MTTNEITQQKGGYLTDLQQLDGATLRNFVDPKHQASPQELQTL 
                 110 
               
               
                   
                   
                   
                 subsp  gallolyticus  TX20005 
                 LAIVKNRNLNPFTKEVYFIKYGNNPAQIVVSKDAFMKRAEQNP 
                   
               
               
                   
                   
                   
                   
                 NYDGFESGVIYENQTGELKSKKGVILPKNCKEVGGWCEVYRK 
                   
               
               
                   
                   
                   
                   
                 DRSRPVYREVELSAYNTGKNWWGKAPGQMIEKVAIVAAVRD 
                   
               
               
                   
                   
                   
                   
                 TFSEDVGGLYTTDEMEQAAPIDVTPQETQEDVIARKQAQIENW 
                   
               
               
                   
                   
                   
                   
                 QNQKAQQEEAQAEPAQEEQTELLDENGELVY 
                   
               
               
                   
               
               
                 SR103 
                 SSAP 
                 recT 
                 
                   Acinetobacter radioresistens 
                 
                 MNAVTQVENQLGLSLKDYDVDQAMWSALTSSIFPGAKPESIV 
                 111 
               
               
                   
                   
                   
                 SK82 
                 MAVEYCKARNLDIMKKPCHIVPMSVKDAKTGNSDWRDVIMPSI 
                   
               
               
                   
                   
                   
                   
                 AEHRITASRSHSYAGIDAPVFGPMVNISFGGVSHTVPEFCTVT 
                   
               
               
                   
                   
                   
                   
                 VYRIIHGEKVAFAHTEYFEEACATVKGGGLNSMWTKRKRGQLA 
                   
               
               
                   
                   
                   
                   
                 KCAEAGALRKAFPEEIGDGYTKEEMEGKIITVGGTEHIQEEAKA 
                   
               
               
                   
                   
                   
                   
                 IEDIRPTLTEKQIETAIKKIKANQGSLDALKAHYNITPEQENYV 
                   
               
               
                   
                   
                   
                   
                 RAQTEVLEHDPV 
                   
               
               
                   
               
               
                 SR104 
                 SSAP 
                 recT 
                   Acinetobacter  sp P8-3-8 
                 MNAPIQHSTIVTAQITQMADLLGLGNVDPVELKNTLIATAFNN 
                 112 
               
               
                   
                   
                   
                   
                 GDKEISDAQMASLLIVAGQYKLNPWTKEIYAFPDKNKGIIPVVG 
                   
               
               
                   
                   
                   
                   
                 VDGWSRIVNSSPNFDGLEFKYSENMVTMEGAKVAAPEWVDCII 
                   
               
               
                   
                   
                   
                   
                 YRKDRERPTVVREYLAECYRPPFKGKYGDVVGPWQSHPSRFL 
                   
               
               
                   
                   
                   
                   
                 RHKATIQCARLAFGFVGIYDQDEAERIQDGGTVRTVQGETGDL 
                   
               
               
                   
                   
                   
                   
                 IPDGYQEFEEAHLNNMRELAFEGMEALETGYAELPKGKCRSHF 
                   
               
               
                   
                   
                   
                   
                 WTMHKESLKAAAQKADQPQGEVYEHSPAE 
                   
               
               
                   
               
               
                 SR105 
                 SSAP 
                 recT 
                   Lactobacillus ruminis  SPM0211 
                 MNQLQKQQTRSITFKANGDDVTLSPSIVRDYLVRGNSKEVTGQ 
                 113 
               
               
                   
                   
                   
                   
                 EIAMFLNLCKFQHLNPFLNEAFIVKFGDKPAQLITSKEAFMKRA 
                   
               
               
                   
                   
                   
                   
                 ESHPQYNGLKAGVIVVNNNGVEFRNGAFTVPDFDQLVGGWCE 
                   
               
               
                   
                   
                   
                   
                 VYRKDRDIPVRVEISLSEFSKGQSTWKTMPATMIRKTAIVNALR 
                   
               
               
                   
                   
                   
                   
                 EAFPETLGALYTEDDDGQMQMQQTKKQVQATENSKAKNKAD 
                   
               
               
                   
                   
                   
                   
                 ALIAQAMDPEHVQQQETEEFQREPRPVDLFNPAEEYSKGE 
                   
               
               
                   
               
               
                 SR106 
                 SSAP 
                 sak 
                   Bacillus  phage 0305phi8-36 
                 MNINECFQVSHYEVDKNLRKLIDEPVNEDLIDKNKYANNSEEV 
                 114 
               
               
                   
                   
                   
                   
                 PDSIYKRVLNKATNYQWSFVPYDISTVEGKYVQFIGLLIVPGYG 
                   
               
               
                   
                   
                   
                   
                 VHTGIGTQKLQKTDNSNALSAAKTYAFKNACKEMGIAPNVGN 
                   
               
               
                   
                   
                   
                   
                 DDFDEALFENFDDDEIEVEEKPKKKPVKKEEKKPAKKPAKKEA 
                   
               
               
                   
                   
                   
                   
                 KPLKERIEEIRQAYELDDDDEVAFIQIWDENIIDLKDMDDKKWK 
                   
               
               
                   
                   
                   
                   
                 AFLQDVEENPEEYEDF 
                   
               
               
                   
               
               
                 SR107 
                 SSAP 
                 ERF 
                   Staphylococcus  phage 92 
                 MNKSETVVEINKAMVAFRKEVKQPEKDKNNPFFKSKYVPLEN 
                 115 
               
               
                   
                   
                   
                   
                 VVEAIDEAATPHGLSYTQWALNDVDGRVGVATMLMHESGEYI 
                   
               
               
                   
                   
                   
                   
                 EYDPVFMNAEKNTPQGAGSLISYLKRYSLSAIFGITSDQDDDGN 
                   
               
               
                   
                   
                   
                   
                 EASGKNNNPKQQTRTQWASSETIGILRKEVIDFTKLIKGTDKEA 
                   
               
               
                   
                   
                   
                   
                 PQNIVEQKFDINNYKLTEKQAAEAIQKIRNNTKTVTGGKQ 
                   
               
               
                   
               
               
                 SR108 
                 SSAP 
                 sak4 
                   Lactobacillus  phage phig1e 
                 MKFYEDGNIPEIPNMYFIYGDGGTGKTSVVKQFVGHKLLFSFD 
                 116 
               
               
                   
                   
                   
                   
                 MSSNVLIGDKDVDVIMFEHRDMPNIQAMVEQYVMQGIQDVKY 
                   
               
               
                   
                   
                   
                   
                 RIIVLDNITALQNLVLENIDDAAKDNRQNYQKLQLWFRDLGTIL 
                   
               
               
                   
                   
                   
                   
                 KESGKTVYATAHQLDNGSSGLSGEGRYQADMNEKTFNAFTSM 
                   
               
               
                   
                   
                   
                   
                 FDLVGRIYLTGGERMIDLDPEKGNHAKNRIDGRKLIKANELIQT 
                   
               
               
                   
                   
                   
                   
                 SKGAK 
                   
               
               
                   
               
               
                 SR109 
                 SSAP 
                 recT 
                   Bacillus  phage 0305phi8-36 
                 MKSRELTPEFKKEFIMKIGGPKGKEAILYNGLLALAHEDPRFGH 
                 117 
               
               
                   
                   
                   
                   
                 IEAYVTQYPSSENKFTCYARAEIFDKEGKRIGMEEADASVNNC 
                   
               
               
                   
                   
                   
                   
                 GKMTAASFPRMALTRAKGRAFRDFLNVGMVTADELQAYEPD 
                   
               
               
                   
                   
                   
                   
                 LASVDSIGKIKRLGKKLGFSRTQLEDFLYDNIGTDKMNELTEPE 
                   
               
               
                   
                   
                   
                   
                 AQEIIDAMNAELADQEPDEEVEEKPARKKRPAKKKSASVDDED 
                   
               
               
                   
                   
                   
                   
                 F 
                   
               
               
                   
               
               
                 SR110 
                 SSAP 
                 gp2.5 
                   Staphylococcus  phage 
                 MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQEAKYSISLIIP 
                 118 
               
               
                   
                   
                   
                 3A,  Staphylococcus  phage 
                 KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTE 
                   
               
               
                   
                   
                   
                 phi7401PVL,  Streptococcus   
                 REDDVNYQDAYFINASSKQAPGIIDQNKIRLTDSGTIVSGDYIRA 
                   
               
               
                   
                   
                   
                   pneumoniae ,  Staphylococcus   
                 SINLFPFNTNGNKGIAVGLNNIQLVEKGEPLGGASAAEDDFDEL 
                   
               
               
                   
                   
                   
                   aureus  (strain NCTC 
                 DTDDEDFL 
                   
               
               
                   
                   
                   
                 8325),  Staphylococcus  phage 
                   
                   
               
               
                   
                   
                   
                 Phi12,  Staphylococcus aureus , 
                   
                   
               
               
                   
                   
                   
                   Staphylococcus  phage 47, 
                   
                   
               
               
                   
                   
                   
                   Staphylococcus  phage tp310-2 
                   
                   
               
               
                   
               
               
                 SR111 
                 SSAP 
                 ERF 
                   Flavobacterium  phage 11b 
                 MKEIATALVKAQKEMTTPKKGSVNPFFKNKYADLNDVLAAIV 
                 119 
               
               
                   
                   
                   
                   
                 PALNNNGIVLLQPLVNIEGKNFVKTVLMHESGEVFESLAEIFCN 
                   
               
               
                   
                   
                   
                   
                 KNNDAQAYGSGVSYARRYSLSSICGIGSEDDDAHTAVNTKPKA 
                   
               
               
                   
                   
                   
                   
                 VPVVLTLTDAVIDSVIAKGIDTIKSCLESIKNGSRIATQVQILK 
                   
               
               
                   
                   
                   
                   
                 LENGSK 
                   
               
               
                   
               
               
                 SR112 
                 SSAP 
                 gp2.5 
                   Acyrthosiphon pisum  secondary 
                 MKIKLNNVRLAFPELFEPTQVSGQGAFKYRANFLIPKSRTDLIE 
                 120 
               
               
                   
                   
                   
                 endosymbiont phage 1 
                 EIKAGIKHVIGEKWGNKDIEKIYNSICNNPNRFCLRDGDSKEYD 
                   
               
               
                   
                   
                   
                 (BacteriophageAPSE-1) 
                 GYAGNLYLSASNKSRPLVIDRNTSPLTAQDGRPYSGCYVNATV 
                   
               
               
                   
                   
                   
                   
                 EFYGYDNNGKGVSASLRGVQFFRDGDAFTGGGVASVEEFDDL 
                   
               
               
                   
                   
                   
                   
                 SMAEEEELLAS 
                   
               
               
                   
               
               
                 SR113 
                 SSAP 
                 sak4 
                   Streptococcus  phage 
                 MKITKATEIKNNDSCYLIYGNPGFGKTSTAKYLPGKTIVINIDK 
                 121 
               
               
                   
                   
                   
                 A25,  Streptococcus pyogenes , 
                 SAKVLRGNENIDIADIDTHKIWGEWLDTVKELLNGAANDYDNIV 
                   
               
               
                   
                   
                   
                   Streptococcus pyogenes  serotype 
                 IDNVSELFRACLANLGREGKNHRVPSQADYQRVDFTILDSLRA 
                   
               
               
                   
                   
                   
                 M2 (strain 
                 LLQLNKRIVFLAWETSDQWTDENGMIYNRAMPDIRTKILNNFL 
                   
               
               
                   
                   
                   
                 MGAS10270),  Streptococcus   
                 GLTDVVARLVKKTTDDGEEVRGFILQPSASVYAKNRLDDRKG 
                   
               
               
                   
                   
                   
                   pyogenes  serotype M4 (strain 
                 CKVEELFETT 
                   
               
               
                   
                   
                   
                 MGAS10750),  Streptococcus   
                   
                   
               
               
                   
                   
                   
                   pyogenes  serotype M3 (strain 
                   
                   
               
               
                   
                   
                   
                 ATCC BAA-595/ 
                   
                   
               
               
                   
                   
                   
                 MGAS315),  Streptococcus   
                   
                   
               
               
                   
                   
                   
                   pyogenes  GA06023,  Streptococcus   
                   
                   
               
               
                   
                   
                   
                   pyogenes  STAB902 
                   
                   
               
               
                   
               
               
                 SR114 
                 SSAP 
                 gp2.5 
                   Prochlorococcus  phage P-55P7 
                 MKNIHVTKEPVTLEGYQAILKPSKFGYSLKAVVGSDIVDALETE 
                 122 
               
               
                   
                   
                   
                   
                 RADCLKWAESKLKNPKRATLKPTPWEEVEEGKFIVKFSWAED 
                   
               
               
                   
                   
                   
                   
                 KRPPVVDTEGTPITNEDVPVYEGSKVKIGFHQKPYILRDGVTYG 
                   
               
               
                   
                   
                   
                   
                 TSLKLSGIQVVSIQSGAGVDTGDEDQDGVAELFGKTQGFKADD 
                   
               
               
                   
                   
                   
                   
                 PNVTPAEEAPVPDDDF 
                   
               
               
                   
               
               
                 SR115 
                 SSAP 
                 sak4 
                   Lactobacillus  phage phij11 
                 MKKIVNFTDGANIFVVLGQVGSGKTHLTLGHKGKKLVISFDGS 
                 123 
               
               
                   
                   
                   
                   
                 YSTLEGHEDEMTVVEPEITDYGNPDKLVSEIDDLAKGCDLVVF 
                   
               
               
                   
                   
                   
                   
                 DNISAVETSLVDAITDGKLGNNTDGRAAYGVVQKLMAKFARW 
                   
               
               
                   
                   
                   
                   
                 AIHFNGDVLFTLWSEVTEEGKEKPAMNAKAFNSVAGYAKLVS 
                   
               
               
                   
                   
                   
                   
                 RTETGEDGYTVVVNPDNRGVIKNRLADKIKKQSIKNDDYWKA 
                   
               
               
                   
                   
                   
                   
                 VEFAKGSKHENA 
                   
               
               
                   
               
               
                 SR116 
                 SSAP 
                 recT 
                 
                   Komagataeibacter oboediens 
                 
                 MNALTVQGHTPAIQITSFEQLVKFADIASRSGMVPSAYSGKPQA 
                 124 
               
               
                   
                   
                   
                   
                 VLIAVQMGSELGLAPMQSLQNIAVINGRPSVWGDALLGLVKAS 
                   
               
               
                   
                   
                   
                   
                 AVCDDVVETMEGEGDALTAICVAKRKGKSPVEARFSVEDAKA 
                   
               
               
                   
                   
                   
                   
                 AGLWNKQGPWKQYPKRMLQMRARGFALRDAFPDVLRGLITA 
                   
               
               
                   
                   
                   
                   
                 EEAGDIPQDDFRSSVSGQRPVDVTPQVRHVEQERAPNYIAYFES 
                   
               
               
                   
                   
                   
                   
                 KLADCRNTTSVDALWKQWNDRIEKAHSAGRPIAQETIERVQE 
                   
               
               
                   
                   
                   
                   
                 MMGERMGEVEQAERQQTEELAAAPVEEPVA 
                   
               
               
                   
               
               
                 SR117 
                 SSAP 
                 rad52 
                   Saccharomyces cerevisiae  (strain 
                 MNEIMDMDEKKPVFGNHSEDIQTKLDKKLGPEYISKRVGFGTS 
                 125 
               
               
                   
                   
                   
                 ATCC 204508/S288c) (Baker\s 
                 RIAYIEGWRVINLANQIFGYNGWSTEVKSVVIDFLDERQGKESI 
                   
               
               
                   
                   
                   
                 yeast),  Saccharomyces cerevisiae   
                 GCTAIVRVTLTSGTYREDIGYGTVENERRKPAAFERAKKSAVT 
                   
               
               
                   
                   
                   
                 YJM1250,  Saccharomyces   
                 DALKRSLRGFGNALGNCLYDKDFLAKIDKVKFDPPDFDENNLF 
                   
               
               
                   
                   
                   
                   cerevisiae  YJM451 
                 RPTDEISESSRTNTLHENQEQQQYPNKRRQLTKVTNTNPDSTKN 
                   
               
               
                   
                   
                   
                   
                 LVKIENTVSRGTPMMAAPAEANSKNSSNKDTDLKSLDASKQD 
                   
               
               
                   
                   
                   
                   
                 QDDLLDDSLMFSDDFQDDDLINMGNTNSNVLTTEKDPVVAKQ 
                   
               
               
                   
                   
                   
                   
                 SPTASSNPEAEQITFVTAKAATSVQNERYIGEESIFDPKYQAQSI 
                   
               
               
                   
                   
                   
                   
                 RHTVDQTTSKHIPASVLKDKTMTTARDSVYEKFAPKGKQLSM 
                   
               
               
                   
                   
                   
                   
                 KNNDKELGPHMLEGAGNQVPRETTPIKTNATAFPPAAAPRFAP 
                   
               
               
                   
                   
                   
                   
                 PSKVVHPNGNGAVPAVPQQRSTRREVGRPKINPLHARKPT 
                   
               
               
                   
               
               
                 SR118 
                 SSAP 
                 recT 
                   Clostridium botulinum  (strain 
                 MNENKELTEQQGANIANDLDTFGSIKGFENTCRMAKALASSTI 
                 126 
               
               
                   
                   
                   
                 Eklund 17B/Type B) 
                 VPKEYHDNIGNCLIALDVANRVGLSPIMVMQNLYVVNGRPAW 
                   
               
               
                   
                   
                   
                   
                 GSQSISALINTSKKYAKPLQYKIDGSGDELSCYAYTFDHEENEIK 
                   
               
               
                   
                   
                   
                   
                 GPVITIKMAKEEGWINKSGSKWKTMPEVMIRYRAASFFGRLHC 
                   
               
               
                   
                   
                   
                   
                 SELLLGIYSADEAVELEPATVIEVTHEEVKQEIKENANQEIIDI 
                   
               
               
                   
                   
                   
                   
                 QIEEVKEDDKEKSKNENPTVKQTVVKTEPF 
                   
               
               
                   
               
               
                 SR119 
                 SSAP 
                 recT 
                   Campylobacter coli  80352 
                 MNTKITNTQNQAVSTSFTQEKIELIRKHFFPVNAKTVEMEYCLN 
                 127 
               
               
                   
                   
                   
                   
                 IANKYNLDPFLKQIFFVPRRSQVEVNGKKEWVDKIDPLVGRDG 
                   
               
               
                   
                   
                   
                   
                 FLAIAHKTGKFGGIRSYSEIKQFPRLNDNGKWEYIQDLVAVCEV 
                   
               
               
                   
                   
                   
                   
                 HRTDSDKPFVVEVAYNEYVQKKASGEATSFWTTKPDTMLKKV 
                   
               
               
                   
                   
                   
                   
                 AESQALRKAFNLSGLYSAEEMGVGMT 
                   
               
               
                   
               
               
                 SR120 
                 SSAP 
                 recT 
                   Helicobacter pullorum  MIT 98- 
                 MNKEIINKENNQVALADAENLAFIKKQFFPVNATEKDIEFCLKV 
                 128 
               
               
                   
                   
                   
                 5489 
                 AQAYSLNPVLREIFFVERMANVNGAWVVKVEPLVSRDGLLSIA 
                   
               
               
                   
                   
                   
                   
                 HKSGKLSGIKSESFLKETPVLINGEWEIKKDLCAVANVYRTDTK 
                   
               
               
                   
                   
                   
                   
                 EVFSAEVFYSEYAQKTKEGKITKFWAEKPHTMLKKVAESQAL 
                   
               
               
                   
                   
                   
                   
                 RKAFNINGIYTPEELDGIKSVGGDVKTLSGVDLEVEADIDEPEIF 
                   
               
               
                   
                   
                   
                   
                 YELDSTPAEADSEKKAVEALGLSVEEKNGYLKIMGNTYKKEA 
                   
               
               
                   
                   
                   
                   
                 HIKALGYTLHKASSGENIWIKKLA 
                   
               
               
                   
               
               
                 SR121 
                 SSAP 
                 ERF 
                   Escherichia  phage Tls 
                 MKFSEQNANVIKALFEARQLFTKVKKDKQNTHLKNKYATLDS 
                 129 
               
               
                   
                   
                   
                   
                 VLDAIMPGLTDKGLFLTQDQKVGEDLKSMTVLTRFIHVESNEW 
                   
               
               
                   
                   
                   
                   
                 VEYSFTLPMQKLDPQGGGSTNSYARRYALCTALGLATADDDA 
                   
               
               
                   
                   
                   
                   
                 NLATKNAQDWKKDLDACDNLTDLQETFKTAYKQSDAANRRII 
                   
               
               
                   
                   
                   
                   
                 KEHYDKLKAKMEIGKARGFNPAEPAANVAKNEKVEKEPQQEV 
                   
               
               
                   
                   
                   
                   
                 KSQSITDFE 
                   
               
               
                   
               
               
                 SR122 
                 SSAP 
                 gp2.5 
                 
                   Bordetella bronchiseptica 
                 
                 MKLKLNNVRLAFPVLFEAKTVNGEGKPAFSASFLIDPSDAQVK 
                 130 
               
               
                   
                   
                   
                 ( Alcaligenes bronchisepticus ) 
                 ALNQAIEQVAKEKWGAKADAVLKQMRAQDKVCLHDGDLKA 
                   
               
               
                   
                   
                   
                   
                 NYDGFPGNLYVSARSATRPLVIDKDRSPLAEADGKPYAGCYVN 
                   
               
               
                   
                   
                   
                   
                 ASIELWAQDNNYGKRVNAGLRGVQFLRDGDAFAGGGVASED 
                   
               
               
                   
                   
                   
                   
                 EFDDITEGAAAADLV 
                   
               
               
                   
               
               
                 SR123 
                 SSAP 
                 ERF 
                   Lactococcus  phage LL-H 
                 MKMQHSDSIKEIFGALSKFRAQVKQPAKTANNPVFNSKYVTLE 
                 131 
               
               
                   
                   
                   
                 ( Lactococcus delbrueckii   
                 GVMQAIDAALPGTGLAYSQLVENGDNGASVSTLITHSSGEWMI 
                   
               
               
                   
                   
                   
                 bacteriophage LL-H) 
                 VGPLTLNPTKRDPQGQGSAITYAKRYQLASAFGISSDIDDDGNA 
                   
               
               
                   
                   
                   
                   
                 GTFGEDSRWQSGYQRSRPKTTHTEAKTLTKAITLASSLAHHSK 
                   
               
               
                   
                   
                   
                   
                 PKSGP 
                   
               
               
                   
               
               
                 SR124 
                 SSAP 
                 ERF 
                   Listeria  phage A500 
                 MKMSESVLELSVALSKFQEKVEQPAKTANNPFFKSSYVPLENV 
                 132 
               
               
                   
                   
                   
                 (Bacteriophage A500) 
                 ISAVKKHAPDLGLSYIQIPLTEENKVGVKTILMHSSGEFVEFDPF 
                   
               
               
                   
                   
                   
                   
                 MLPLDKNTAQGAGSALTYARRYTLSSAFGIASDEDDDGNGAS 
                   
               
               
                   
                   
                   
                   
                 GNTKANKSSKNYQQTKQTQPTQQSDNLASPAQRKAIFAKASV 
                   
               
               
                   
                   
                   
                   
                 VGDPFGHDAKYVLESYKITDTKSMSKGEASALIKKEDAEIEAQ 
                   
               
               
                   
                   
                   
                   
                 KQVN 
                   
               
               
                   
               
               
                 SR125 
                 SSAP 
                 recT 
                 
                   Acinetobacter radioresistens 
                 
                 TTKEWKWNERARKSLPEEKVHTVKLRNITCKAWAIETATGER 
                 133 
               
               
                   
                   
                   
                 SK82 
                 LESAEISMEMAVKEGWYQKNGSKWQTMPEQMLRYRAASFFG 
                   
               
               
                   
                   
                   
                   
                 RIYAPEVLMGIRTQEEEQDAIIDVTPEPVQQTSPVTTSDLKANV 
                   
               
               
                   
                   
                   
                   
                 VKEAPVEQQAKQQPVQEEKKPRARKQPKVIETENVQNSTENE 
                   
               
               
                   
                   
                   
                   
                 VVDAELTVEDLKRLQQEAENLIQQNKTSETAQVDVNKFAEIKK 
                   
               
               
                   
                   
                   
                   
                 SYMKTLTSTVQASSINGLKSQIEKE 
                   
               
               
                   
               
               
                 Strep01 
                 SSAP 
                 recT 
                 
                   Streptomyces longwoodensis 
                 
                 MSLTTLKERVQQRAAAASSDGIPPGQADEDTPAGRDHDAEQFR 
                 134 
               
               
                   
                   
                   
                   
                 ADIAAALPAHVSVDRFLAVFRPVLPGLRKCTPASVRQAVITAA 
                   
               
               
                   
                   
                   
                   
                 RFGLLPDGEEAVITADDGIATFLATYHGYIELMWRSGMVKSIV 
                   
               
               
                   
                   
                   
                   
                 AKVVYDGDEWDYVPTAPVGQDFTHRPKLGSPKDRTPLFAYTF 
                   
               
               
                   
                   
                   
                   
                 AWLDGGARSDVAIVTVEEAEAIRDEFSKAYQRAEANGQKNSL 
                   
               
               
                   
                   
                   
                   
                 WHTRFPDMLLKTAIRRAAKLLPKSAELRALVAVERAADEGHT 
                   
               
               
                   
                   
                   
                   
                 QILAALTPEALALEAEARAAARAAEASQDVPPPRLPVKRGRAR 
                   
               
               
                   
                   
                   
                   
                 PRRRHRDKNKATRR 
                   
               
               
                   
               
               
                 Strep02 
                 SSAP 
                 recT 
                 
                   Streptomyces albus 
                 
                 MSQISNALATRDQGPAAQIEQYRDEYAALVPSHVNADQWVRL 
                 135 
               
               
                   
                   
                   
                   
                 AVGAVRGDEKLMEAAQNDIGLFLREMKTAARLGLEPGTEQFY 
                   
               
               
                   
                   
                   
                   
                 LTPRKSKPHGGRKVIKGIVGYQGIVELIYRAGAASTVIVEAVRE 
                   
               
               
                   
                   
                   
                   
                 NDTFRYVPGRDDRPVHEIDWFANDRGPLVGVYAYAVMKDGA 
                   
               
               
                   
                   
                   
                   
                 VSKVVVLNRSRVMEFKAKSDSKHSEYSPWNTNEEAMWLKSA 
                   
               
               
                   
                   
                   
                   
                 VRQLAKWVPTSAEYRRDQLLAHTETADSVVASVSTAPLPPQPS 
                   
               
               
                   
                   
                   
                   
                 ALDDADPDDDGPIDAELVD 
                   
               
               
                   
               
               
                 Strep03 
                 SSAP 
                 recT 
                   Streptomyces noursei  ATCC 
                 MTSPIRAAVARRAGDPAALISQYTADFAAVEPSHIKPATFVRLA 
                 136 
               
               
                   
                   
                   
                 11455 
                 QGILRRDEKLAQAAANDPGQFMSVLLDAARLGLEPGTEAYYL 
                   
               
               
                   
                   
                   
                   
                 VPFKGRVQGIVGWQGEIELMYRAGAISSVIVETVREDDVFVWT 
                   
               
               
                   
                   
                   
                   
                 PGLVDRETPPRWEGPMSYPFHEVEWAGDRGPLRLVYAYAVM 
                   
               
               
                   
                   
                   
                   
                 KGGATSKVVVLNAQDIERAKKTSQGADSPSSPWRQHEAAMWS 
                   
               
               
                   
                   
                   
                   
                 KTAVHRLAKYVPTSAEYITAQVRAVRQADALSAPPVEEVVDV 
                   
               
               
                   
                   
                   
                   
                 ELVGDGQEQEARR 
                   
               
               
                   
               
               
                 Strep04 
                 SSAP 
                 recT 
                 
                   Streptomyces albulus 
                 
                 MNNPSEEPSVDGAPTAQRPGGENASPDQAVTPSAIILRAEQTEF 
                 137 
               
               
                   
                   
                   
                   
                 DHRQRMALALISPELKKASRPELAVFFHHCVRSGLDPFARQIY 
                   
               
               
                   
                   
                   
                   
                 MIGRKNKGRDREENPITWTIQTGIDGFRTIAHRAAERTGERISYE 
                   
               
               
                   
                   
                   
                   
                 DTVYYAADGTTSDVWLADEPPAAVKVTVLRGTSRFPLVARW 
                   
               
               
                   
                   
                   
                   
                 AEFAPEHWDNKQGKYVVAAMWKQMPAHMLRKCAEAGSLR 
                   
               
               
                   
                   
                   
                   
                 MAAPQDLSGVYADEEMEQADAEVVAGEAKEASSRLRAAAGL 
                   
               
               
                   
                   
                   
                   
                 DDAHSEAESNAEVEEPEAPTPKKSRPKPATEASTQSEGSKRPRK 
                   
               
               
                   
                   
                   
                   
                 RTAGKRAANKAADSGAGG 
                   
               
               
                   
               
               
                 Strep05 
                 SSAP 
                 recT 
                   Streptomyces rimosus  subsp 
                 MSQISNAIATRDTGPAAQIEQYRDEYAALVPSHINADQWIRLAV 
                 138 
               
               
                   
                   
                   
                 
                   pseudoverticillatus 
                 
                 GAIRGNKDLEKAARTDVGIFLRELKTAARLGLEPGTEQFYLTPR 
                   
               
               
                   
                   
                   
                   
                 KSKAHGVALIIKGIVGYQGIVELIYRAGAVSSVIVETVREHDTFS 
                   
               
               
                   
                   
                   
                   
                 YVPGRDERPVHEIDWFGADRGALVGVYAYAVMKDGATSKVV 
                   
               
               
                   
                   
                   
                   
                 VLNRARVMEIKAKSDSKDSEYSPWKTSEEAMWLKSAVRQLAK 
                   
               
               
                   
                   
                   
                   
                 WVPTSAEYMREQLRAQAEVAAETPSVASAPLPPQPSALDDYDP 
                   
               
               
                   
                   
                   
                   
                 ADDGPIEGELVD 
                   
               
               
                   
               
               
                 Strep06 
                 SSAP 
                 recT 
                 
                   Streptomyces cyaneogriseus 
                 
                 MSQIGNAIEKRDQGPAAQIEQYRDEYAALVPSHVNADQWIRLA 
                 139 
               
               
                   
                   
                   
                 subsp  noncyanogenus   
                 VGAIRGNKDLEQAARNDVGVFLRELKTAARLGLEPGTEQFYLT 
                   
               
               
                   
                   
                   
                   
                 PRKSKAHGYKLIIKGIVGYQGIVELIYRAGAVSTVIVKAVRERD 
                   
               
               
                   
                   
                   
                   
                 TERYVPGRDDRPIHEIDWFGTDRGPLVGVYAYAVMKDGAVSK 
                   
               
               
                   
                   
                   
                   
                 VVVLNRTRVMEIRAKSDSKDSEYSPWQTNEEAMWLKSAVRQL 
                   
               
               
                   
                   
                   
                   
                 AKWVPTSAEYMREQLRAQAEVAGELAAPSVMGAPAMPQPSV 
                   
               
               
                   
                   
                   
                   
                 LDDTDPDDEPIDGELVD 
                   
               
               
                   
               
               
                 Strep07 
                 SSAP 
                 recT 
                   Streptomyces  sp HPH0547 
                 MSSQISNAIATRDNGPTAQLKQYRDEYAALVPSHINLDQWIRL 
                 140 
               
               
                   
                   
                   
                   
                 ATGALRGDEKLMEAAQNDVGVYLREMKTAARLGLEPGTEQF 
                   
               
               
                   
                   
                   
                   
                 YLTPRKSKAHDGRPIIKGIVGYQGIIELIYRAGAVSSVVVEAVRR 
                   
               
               
                   
                   
                   
                   
                 ADTFHYVPGRDERPIHEIDWFNDDRGPLVGVYAVAVMKDGAT 
                   
               
               
                   
                   
                   
                   
                 SKVVVLNKRQVMEAKAKSDSRNSQYSPWQTNEEAMWLKTAV 
                   
               
               
                   
                   
                   
                   
                 RRLAKWVPTSAEYMREQLRAAKEVAAEPTPDAPSLPPVQAAP 
                   
               
               
                   
                   
                   
                   
                 LDDVDPDTGEVLEGELLDEPSTT 
                   
               
               
                   
               
               
                 PF001 
                 SSAP 
                 recT 
                   Hungatella hathewayi  DSM 13479 
                 MDELTLNQPVTSLSSGVFSSAESFQELFNIGKMFSASTLVPQAY 
                 141 
               
               
                   
                   
                   
                   
                 QGKPMDCTIAVDMANRMGVSPMMVMQNLYVVKGKPSWSGQ 
                   
               
               
                   
                   
                   
                   
                 ACMSMIRASKEFKNVRLVYTGDKGTDSWGCYVQAEHRETGEP 
                   
               
               
                   
                   
                   
                   
                 VKGTEVTIKMAKEEDWYGKTGSKWKTMPEQMLAYRAAAFFA 
                   
               
               
                   
                   
                   
                   
                 RVYIPNSLMGLHVEGEAEDITNESEIPEIPDIFGEKEKEAQV 
                   
               
               
                   
               
               
                 PF002 
                 SSAP 
                 recT 
                   Ureaplasma urealyticum  serovar 
                 MVKDDKLVPSIRLLTPNELSEQWANPNSEINQITRAVLTIQGIDL 
                 142 
               
               
                   
                   
                   
                 10 (strain ATCC 33699/ 
                 KAIDLNQAAQIIYFCQANNLNPLNKEVYLIQMGNRLAPIVGIHT 
                   
               
               
                   
                   
                   
                 Western),  Ureaplasma   
                 MTERAYRTERLVGIVQSYNDVNKSAKTILTIRSPGLKGLGTVEA 
                   
               
               
                   
                   
                   
                   urealyticum  serovar 7 str ATCC 
                 EVFLSEYSTNKNLWLTKPITMLKKVSLAHALRLSGLLAFKGDT 
                   
               
               
                   
                   
                   
                 27819,  Ureaplasma parvum   
                 PYIYEEMQQGEAVPNKKMFTPPVAEVIEPAVENIKKVDFNEF 
                   
               
               
                   
                   
                   
                 serovar 3 (strain ATCC 
                   
                   
               
               
                   
                   
                   
                 700970),  Ureaplasma urealyticum   
                   
                   
               
               
                   
                   
                   
                 serovar 8 str ATCC 
                   
                   
               
               
                   
                   
                   
                 27618,  Ureaplasma urealyticum   
                   
                   
               
               
                   
                   
                   
                 serovar 4 str ATCC 27816 
                   
                   
               
               
                   
               
               
                 PF003 
                 SSAP 
                 recT 
                   Clostridium  sp FS41 
                 MAENEKQALLQEENKSENVVSTVKRTALATNPFSDTDQFNNIF 
                 143 
               
               
                   
                   
                   
                   
                 KMAQLISQSDMIPATYKGKPMNCVIALEQANRMGVSPLMVMQ 
                   
               
               
                   
                   
                   
                   
                 NLYVVKGVPSWSGQGCMMIIQGCGKFRDVDYVYSGEKGTDSR 
                   
               
               
                   
                   
                   
                   
                 SCKVVATRISDGKRIEGTEITMQMVKSEGWISNTKWKNMPEQ 
                   
               
               
                   
                   
                   
                   
                 MLGYRAATFFARMYCPNELNGFATEGEAEDMNHKPQRIEAIN 
                   
               
               
                   
                   
                   
                   
                 VLGDTAHE 
                   
               
               
                   
               
               
                 PF005 
                 SSAP 
                 recT 
                   Clostridium  sp CAG:470 
                 MSNEVQKNNELMVKFDIDGNEIKLTPSIVQEYIVGTDAKITNQE 
                 144 
               
               
                   
                   
                   
                   
                 FKLFTELCKVRKLNPFLREAYLIKYKAGVPAQLVVGKDAILKR 
                   
               
               
                   
                   
                   
                   
                 AVLNPNYDGMESGIIVQKEDGSVEERQGTFRLGNEQLVGGWA 
                   
               
               
                   
                   
                   
                   
                 RVFRKDWTHPTYSSVSFNEVAQKTGQGQLNSNWGSKGATMV 
                   
               
               
                   
                   
                   
                   
                 EKVAKVRALRETFVEDLAGMYEAEEMQQEISQQEPIEVQAEIE 
                   
               
               
                   
                   
                   
                   
                 EQTENTKEVSMNEL 
                   
               
               
                   
               
               
                 PF006 
                 SSAP 
                 recT 
                 
                   Coriobacteriales bacterium 
                 
                 MASKNEAIEVSPAEIASVKEKPASIVKAEKAKKEPCALVKYEDAE 
                 145 
               
               
                   
                   
                   
                 DNF00809 
                 GREVVLTREDIINTISSNPRITDKEIKEFIELARAQKLNPFTREI 
                   
               
               
                   
                   
                   
                   
                 FITKYGDYPATFIVGKDVFTKRAQSNPLFKGMQAGIIVQRGNA 
                   
               
               
                   
                   
                   
                   
                 VDQREGSATFGDEMLIGGWCKVYVQGYDVPIYDSVSFNEYAA 
                   
               
               
                   
                   
                   
                   
                 RKTDGTLNAMWASKPATMIRKVAIVHALREAFPSDEQGLYDQ 
                   
               
               
                   
                   
                   
                   
                 SEMGLSGQGGE 
                   
               
               
                   
               
               
                 PF007 
                 SSAP 
                 recT 
                 
                   Paeniclostridium sordellii 
                 
                 MSKLKSALQSKEAKGDGLTVSKSYAMKQLTIKMKNDIKEALP 
                 146 
               
               
                   
                   
                   
                 ( Clostridium sordellii ) 
                 SHFCIDNFQKSAINTYNLDKSLQECEATTFISAMIECAKEGLEPN 
                   
               
               
                   
                   
                   
                   
                 NILGQAYLVPVCVDGVNKVEFQIGYKGLIELAYRSGKIKSLYA 
                   
               
               
                   
                   
                   
                   
                 NEVFEKDEFHIDYGLDQKLIHKPFLGGDRGEVIGYVAVYQMDN 
                   
               
               
                   
                   
                   
                   
                 RGASFVFMTRDEILGHSKKYSRSFGCDLWESEFDAMAKKTVIK 
                   
               
               
                   
                   
                   
                   
                 KLLKYAPLSIELQKSVSIDESVKGVGCI 
                   
               
               
                   
               
               
                 PF008 
                 SSAP 
                 recT 
                   Streptococcus infantis  SK970 
                 MTNNQLSTQQTKRDISVNALDWTFEDIKRYFDPQNLLTEKQVG 
                 147 
               
               
                   
                   
                   
                   
                 QALSLIKGRNLNPLANEVYIVAYKNRNGGTEFSLIVSKEAFLKR 
                   
               
               
                   
                   
                   
                   
                 AAQSKNYEGFEAGVVAVDKDGVMHERKGALMLPGDTLVGG 
                   
               
               
                   
                   
                   
                   
                 WARVYRKNFKVPVEIQVSLEEYNKKQSTWNSMPATMIRKTAL 
                   
               
               
                   
                   
                   
                   
                 VNALREAFPEDLGNMYTEDDGGETFDRIKDVTPQESREDVVAR 
                   
               
               
                   
                   
                   
                   
                 KMAQIEQFNKEQAHTDPEPTQNEEPIQGELLDGELEY 
                   
               
               
                   
               
               
                 PF009 
                 SSAP 
                 recT 
                   Leptotrichia goodfellowii  F0264 
                 MGRLTQNEANDKLIIFKVGNDEVKLSNNIVKRYLVTGQGNVT 
                 148 
               
               
                   
                   
                   
                   
                 DEEIMYFMKLCKARNLNPFVRDAYLIKYSDKDAATIVVAKDAI 
                   
               
               
                   
                   
                   
                   
                 EKRAIQHPKYNGKEVGLYIIKKETGDLEKRNGTIYLKEKEEIAG 
                   
               
               
                   
                   
                   
                   
                 AWCTVYRKDWDNPVTVEVNFDEYVGRKKDGTANINWANRP 
                   
               
               
                   
                   
                   
                   
                 VTMITKVAKAQALREAFIEEISGMYEAEEAGINVNDLDDTPIEQ 
                   
               
               
                   
                   
                   
                   
                 KDMQNMPDVTNTVQETEIDDEDLKKELFDNENKKNPLE 
                   
               
               
                   
               
               
                 PF010 
                 SSAP 
                 recT 
                   Lactobacillus rossiae  DSM 15814 
                 MAVVKRLKGVKSIEAYVVRQGNDFDVASEGEQVVVTKWQM 
                 149 
               
               
                   
                   
                   
                   
                 HVADLDKPIAYAVCVIEKEDGTKSFTIMTKKQIDVSWSHAKTT 
                   
               
               
                   
                   
                   
                   
                 KVQREYPDEMAKRTVINRAAKLFINTSDDSDLFVQAVNDTTSD 
                   
               
               
                   
                   
                   
                   
                 EYDNDSRKDVTPSAESKGTQDLLDGFQSSNQSQQPSATSQSSQ 
                   
               
               
                   
                   
                   
                   
                 PVSTATSAEKSANPSAESAVTSKTAKPSSTAEDIINGYEQSQSSK 
                   
               
               
                   
                   
                   
                   
                 GADTVDEDGHNVVIDHEDEEPDVHQGDIFNQHDNPQS 
                   
               
               
                   
               
               
                 PF011 
                 SSAP 
                 recT 
                   Paenibacillus  sp FSL R7-0331 
                 MTIAIETQVQETLDRILDSKHDALPSDFNKKRFSENCKVYVFDD 
                 150 
               
               
                   
                   
                   
                   
                 KDLHKYTADEIAANLFKGAVLGLDFLAKECHLISGGVDLKFQT 
                   
               
               
                   
                   
                   
                   
                 DYKGEMKLTKKYSIRPLLDVYAKNVREGDEFREEVIEGRQVIH 
                   
               
               
                   
                   
                   
                   
                 FAPRPFNTSKIIGSFAVAFFHDGGMVCESIPAGEIEEIRKNYGKA 
                   
               
               
                   
                   
                   
                   
                 LGDAWDKSQGEMYKRTVLRRLCKTIETDFDAEQRLVYDAGG 
                   
               
               
                   
                   
                   
                   
                 AFEFTKQPARSRQQSPFNPPEESEVIQNDRAAETDQG 
                   
               
               
                   
               
               
                 PF012 
                 SSAP 
                 recT 
                 
                   Nocardia terpenica 
                 
                 MSEISKAVATQQNPLAVVARYKRELGTVLPTVLRQDPDRWLM 
                 151 
               
               
                   
                   
                   
                   
                 AAENAARKNPDIMAVTKADQGASYMRALVECARLGHEPGSK 
                   
               
               
                   
                   
                   
                   
                 DEHFIKRGNAISGEESYRGIIKRVLNSGFYRSVVARTVFSNDTYS 
                   
               
               
                   
                   
                   
                   
                 FDPLTDIVPNHVPAQGDRGKPLSAYAFAVHWDGTPSTVAEATP 
                   
               
               
                   
                   
                   
                   
                 ERIATAKAKSFASDKPTSPWQLPTGVMYRKTAIRELEPYVHVA 
                   
               
               
                   
                   
                   
                   
                 PEPQPRRHLDGTVGGIPATDFDVDDGDVLDITADQLAEAGEIV 
                   
               
               
                   
               
               
                 PF014 
                 SSAP 
                 recT 
                   Dermabacter  sp HFH0086 
                 MSNALTITQDQTEFTPKQLSVLENLGVQGAAPQEVAMFFDYCQ 
                 152 
               
               
                   
                   
                   
                   
                 RTGLSPWARQIYMIGRWDRNLGRKKYAVQVSIDGQRLVAERS 
                   
               
               
                   
                   
                   
                   
                 GVYEGQTAPQWCGPDGQWVDVWLANEPPQAARVGVWRKSF 
                   
               
               
                   
                   
                   
                   
                 REPAYGVARLSSYMPVTRDGKPQGLWGTMPDVMLAKCAESL 
                   
               
               
                   
                   
                   
                   
                 ALRKAFPLELSGLYTSEEMQQADAPRTEPAPVDEDVVDAEIVD 
                   
               
               
                   
                   
                   
                   
                 DEERMQWVEAIQAAETTDVLRKMWADIKTCPDALQAELRELI 
                   
               
               
                   
                   
                   
                   
                 PARAKELAA 
                   
               
               
                   
               
               
                 PF015 
                 SSAP 
                 recT 
                   Leifsonia xyli  subsp  xyli , 
                 MAVKKNPTIEDYLIKVEPEFQRALGASMDAAKFAQDALTAIKQ 
                 153 
               
               
                   
                   
                   
                   Leifsonia xyli  subsp  xyli   
                 NPKIGHSDPRSLFGALFLAAQLKLPVGGPLAQFHLTTRTVKGNL 
                   
               
               
                   
                   
                   
                 (strain CTCB07) 
                 TVVPIVGYGGYVQLIMNTGLYSRVSAFLIHAGDYFVTGANSER 
                   
               
               
                   
                   
                   
                   
                 GEFYDFRRADSDRGEVKGVIAYAKVKGHNESSWVYIDAETMR 
                   
               
               
                   
                   
                   
                   
                 AKHRPKYWESTPWADDAGEMFKKTGIRVLQKYLPKSVESLNV 
                   
               
               
                   
                   
                   
                   
                 ALAASADQAIVRKVDGVPDLDIQHDRDTETVAVPEQPVSVPQP 
                   
               
               
                   
                   
                   
                   
                 GDET 
                   
               
               
                   
               
               
                 PF017 
                 SSAP 
                 recT 
                 
                   Lactobacillus shenzhenensis 
                 
                 MSAVSESKDLQHVDQLSVLNSAMTAASLNLPINQNLGFFYLVP 
                 154 
               
               
                   
                   
                   
                 LY-73 
                 YKGIAQAQMGYKGYIQLAQRSGQYQRLNAIPVYADEFGSWNP 
                   
               
               
                   
                   
                   
                   
                 LTEELDYTPHFEDRKASDKPVGYVGFFKLANGFEKTVYWSRK 
                   
               
               
                   
                   
                   
                   
                 QIEAHRDRFSKSSKSSASPWNTDFDAMALKTVLRNLITKWGPM 
                   
               
               
                   
                   
                   
                   
                 TTDIQRANDADEGDYKNDLSTDTSEPKDVTPGASLEQFLGETD 
                   
               
               
                   
                   
                   
                   
                 QQQKPATKPAPKKKAEEAKPNDLKPDVTHDPNEHTEQTSLSD 
                   
               
               
                   
                   
                   
                   
                 DDLPFD 
                   
               
               
                   
               
               
                 PF018 
                 SSAP 
                 recT 
                 
                   Pseudoalteromonas lipolytica 
                 
                 MSLSLQEYQNLLYGKLTACKGQFDAYLSENGYKLDFNTELNY 
                 155 
               
               
                   
                   
                   
                 SCSIO 04301 
                 VYQIVMSGLNVEYSFPYTPVESVISSFLKAAKIGLSLCPTEQLCE 
                   
               
               
                   
                   
                   
                   
                 LKTEYSESSGQYVTQLGLGYKGILKLAYRSGKVKQINANVFYE 
                   
               
               
                   
                   
                   
                   
                 KDTFQYNGVNSKVTHTTTVLSKAMRGQLAGGYCQTELIDGSF 
                   
               
               
                   
                   
                   
                   
                 KTTVMPPEEILAIEEQGKVMGNEAWLSVHVDQMREKTLIKRH 
                   
               
               
                   
                   
                   
                   
                 WKTLCPCIVRDSVMNDPMLFDDQDCQHSSNQQAYEEQFESAY 
                   
               
               
                   
                   
                   
                   
                 SREAY 
                   
               
               
                   
               
               
                 PF019 
                 SSAP 
                 recT 
                   Acetobacter orientalis  21F-2 
                 MSNAVATHNPVLQPNNFQELIGFAKMAAASDLMPKDYKGKPE 
                 156 
               
               
                   
                   
                   
                   
                 NIMIAVQMGSELGLAPMQAIQNIAVINGRPSVWGDAMLALVR 
                   
               
               
                   
                   
                   
                   
                 GSGKCSSVKEIFEGDGDNLAAVCVVRRVDGDEVRGEFSVADA 
                   
               
               
                   
                   
                   
                   
                 KRANLWSKQGPWQQYPRRMLQMRARGFALRDAFPDVLRGLI 
                   
               
               
                   
                   
                   
                   
                 GAEEAQDIPADPIDVTPRHRHVEPPKTIDHKQIFSDRLEGCPDAL 
                   
               
               
                   
                   
                   
                   
                 SVDNQWTVWESTIQKAFDAGRPIPEAVQDAVRDMIAAKRAEF 
                   
               
               
                   
                   
                   
                   
                 DRQATEAPIEEPVA 
                   
               
               
                   
               
               
                 PF020 
                 SSAP 
                 recT 
                   Collinsella stercoris  DSM 13279 
                 MNQIVKFTDDSGLAVQVTPDDVRRYICENATEKEVGLFLQLCQ 
                 157 
               
               
                   
                   
                   
                   
                 TQRLNPFVKDAYLVKYGGAPASMITSYQVFNRRACRDANYDG 
                   
               
               
                   
                   
                   
                   
                 IKSGVVVLRDGDVVHKRGAACYKKAGEELIGGWAEVRFKDG 
                   
               
               
                   
                   
                   
                   
                 RETAYAEVALDDYSTGKSNWAKMPGVMIEKCAKAAAWRLAF 
                   
               
               
                   
                   
                   
                   
                 PDTFQGMYAAEEMDQAQQPEQVRAQAEQPVDLQPIRELFKPY 
                   
               
               
                   
                   
                   
                   
                 CEHFGITPAEGMTAVCGAVGAEGMHSMTEQQARRARAWMEE 
                   
               
               
                   
                   
                   
                   
                 EMAAPAVEAEYEVVDEGEVF 
                   
               
               
                   
               
               
                 PF021 
                 SSAP 
                 recT 
                   Lactobacillus capillatus  DSM 
                 MANEVAEKNEVVYLAGNEEVKLKPSTVRDFLTSGNGNITSQE 
                 158 
               
               
                   
                   
                   
                 19910 
                 AFMFIKLCEYQHLNPFLNEAYLIKFGNKPAQIITSKEAFMKRAE 
                   
               
               
                   
                   
                   
                   
                 SHPAYNGVKAGLIVLRNSDVKYTKGAFKLPTDKILGAWAEVN 
                   
               
               
                   
                   
                   
                   
                 RKDRDEPHHIEISMEEFSKQQSTWKSMPATMIRKTAIVNALREA 
                   
               
               
                   
                   
                   
                   
                 FPETLGGMYTEDDKNPNEAVKESEPVDDKAESVVDDLVKDIKP 
                   
               
               
                   
                   
                   
                   
                 KNEPAEKEGEPKESEQYEEQETTFEEVNSKEAEKDGDSNSEEQ 
                   
               
               
                   
                   
                   
                   
                 TSLFKGATIQPNV 
                   
               
               
                   
               
               
                 PF022 
                 SSAP 
                 recT 
                   Treponema socranskii  subsp 
                 MNEIIKKEATEIITPVDEKTIMEYLDTTGLTKSLLPKEKAMFVN 
                 159 
               
               
                   
                   
                   
                   socranskii  VPI DR56BR1116 = 
                 MARLYGLNPFKREIYCTVYGEGQYRQCSIVTGYEVYLKRAERI 
                   
               
               
                   
                   
                   
                 ATCC 35536 
                 GKLDGWQAQITGSLQDGTLAATVTIWRKDWTHPFTHTAFYTE 
                   
               
               
                   
                   
                   
                   
                 CVQTSKKTGEPNAIWRKMPSFMVRKVAIAQAFRLCFSDEFGG 
                   
               
               
                   
                   
                   
                   
                 MPYTNDEMGVDAPKERDITHEATATIADEAEAPSAEVKNEPKP 
                   
               
               
                   
                   
                   
                   
                 ADVVQQLETLLMKYESQLSGKPYELAEEALRTGSDAEVIAMY 
                   
               
               
                   
                   
                   
                   
                 ERVVSYLKRKGIQVGK 
                   
               
               
                   
               
               
                 PF023 
                 SSAP 
                 recT 
                 
                   Bifidobacterium magnum 
                 
                 MTSSTLVPSTLEEQKTYAELVAQSTGMVPAAYQGKPANIFVAI 
                 160 
               
               
                   
                   
                   
                   
                 QVGSSLGLEPMASLQGINVIQGKPTLSAQAMLGLLKSQHFKVH 
                   
               
               
                   
                   
                   
                   
                 ITKDDENQRVTVEVIDPDDPDYSTVSVWDMAKARKAGLGGDQ 
                   
               
               
                   
                   
                   
                   
                 YWTKQPMTMLKWRAVSEAAREAAPHLFLGLGGAYTKEELEE 
                   
               
               
                   
                   
                   
                   
                 SVTVENVEETPRPRSPYSSYTRKPEPVEPEVVEAEPVEPEPINL 
                   
               
               
                   
                   
                   
                   
                 IIQTMRENGVDTAQKARLVLTQIVGVENVNDIPADKLETLCANL 
                   
               
               
                   
                   
                   
                   
                 DGFAHDIQQTLGDNK 
                   
               
               
                   
               
               
                 PF024 
                 SSAP 
                 recT 
                   Frateuria aurantia  (strain ATCC 
                 MSEISVARAQNLAQIERVNALLPTSIGEAMQLAEFMAKSDLLPP 
                 161 
               
               
                   
                   
                   
                 33424/DSM 6220/NBRC 3245/ 
                 HLKGRQGDCLLVVMQAQRWGMDALSVAQCTSVVHGRLCYE 
                   
               
               
                   
                   
                   
                 NCIMB13370) ( Acetobacter   
                 GKLVAAALYSQKAIDGRLHYEISGHGQDASIVVTGTPRGTGQT 
                   
               
               
                   
                   
                   
                   aurantius ) 
                 QSVSGSVRKWRTITMKKQDGAPPKRVDNAWDTIPEDMLVYRG 
                   
               
               
                   
                   
                   
                   
                 TRQWARRYAPEVMLGVQTPDEVDDTPMQTTVIHSTAASSPAIE 
                   
               
               
                   
                   
                   
                   
                 PLIPYPEEEFSKNFDTWLGRIQCGRNSAEEVIAKLQTKYTLTPG 
                   
               
               
                   
                   
                   
                   
                 QLGAIRDLETTEAEVVE 
                   
               
               
                   
               
               
                 PF025 
                 SSAP 
                 recT 
                   Parcubacteria bacterium  32_520 
                 MKKETTQNKAKKITKKASSKKKETKKIVVKEQQITDPALLTKA 
                 162 
               
               
                   
                   
                   
                   
                 KIDLIKRTVAKGATDDELQMFLTVAARAGLDPFTKQIHLVKRH 
                   
               
               
                   
                   
                   
                   
                 STAEGRDIATIQTGIDGYRAIAEKTGKYGGSDDATFTFADGQDK 
                   
               
               
                   
                   
                   
                   
                 IPTSATVKVYKIIGDKVIEIKATAYWDEFAPTNEKLAFMWRKM 
                   
               
               
                   
                   
                   
                   
                 PKLMLAKCAEALALRKAFPNVLSGIYTHEEMEQLDEEKNVND 
                   
               
               
                   
                   
                   
                   
                 KKATLANSKAYIVSAKSLDELKGFRERIEKSKIDKATKEKLLKY 
                   
               
               
                   
                   
                   
                   
                 CQEKEVELSAIEAEIESV 
                   
               
               
                   
               
               
                 PF026 
                 SSAP 
                 recT 
                   Helicobacter  sp MIT 05-5294 
                 MNEVAKLNESRASNQKMIATILESDSSHKKLNDFFAGDSAKVQRF 
                 163 
               
               
                   
                   
                   
                   
                 KSSLINIAGNSILSSCSPASIIRSAFSLAEIGLDINQTLKQSYVL 
                   
               
               
                   
                   
                   
                   
                 KYGIEAEPVISYKGWQSILEKVGKKSRAFCVFKCDTFNIDFSTFE 
                   
               
               
                   
                   
                   
                   
                 GNLTFVPNYAQRDETNRKWFNDNLLGVVVLIKDKDASEIVNF 
                   
               
               
                   
                   
                   
                   
                 VSAKKIDKIKGCSPSVKKGRNSPYDEWFEEMYLAKAIKYVLSK 
                   
               
               
                   
                   
                   
                   
                 QALTEKEETIARAIDIENEVEAKIQKEASNYEIKDLEELMQDKE 
                   
               
               
                   
                   
                   
                   
                 VMAMTLDAEEGIPQI 
                   
               
               
                   
               
               
                 PF027 
                 SSAP 
                 recT 
                 
                   Mycobacterium brisbanense 
                 
                 MTETAVAKPEQRPTTISQVLQVMIPELTRAIPKGMDADRLARIV 
                 164 
               
               
                   
                   
                   
                   
                 QTEIRKSRNAKAAGITRQSLDDCTQESFAGALLTASALGLEPGI 
                   
               
               
                   
                   
                   
                   
                 NGECYLVPYRDTRRRVVECQLIIGYQGIVKLFWQHPRARGIKT 
                   
               
               
                   
                   
                   
                   
                 DWVGANDHFEYEDGENTVLVHRKAADRGEPIAFWAVVKVAD 
                   
               
               
                   
                   
                   
                   
                 ADPLVTVLTADEVKELRKGKVGSSGDIKDVQHWMERKTVLK 
                   
               
               
                   
                   
                   
                   
                 QGLKLAPKTTRLDAAIVNDDRAGTDLSRSQALMLPPGVQSTAD 
                   
               
               
                   
                   
                   
                   
                 YIDGTVDEEPQGYEEIAETEAQVQ 
                   
               
               
                   
               
               
                 PF028 
                 SSAP 
                 recT 
                   Capnocytophaga  sp oral taxon 338 
                 MSAITQTTDKRLGNFLNQANTADFLTKTLGARKSEFVSNLLAIS 
                 165 
               
               
                   
                   
                   
                 str F0234 
                 DADKNLSQCDPSEVMKCAMNATALNLPLSKNLGYAVVIPYKD 
                   
               
               
                   
                   
                   
                   
                 WKTQEVHPQFQIGYKGFVQLAIRSGQYRTINTCEVREGEIKRN 
                   
               
               
                   
                   
                   
                   
                 KFTGHTEFLGENPEGKVIGYLAYIELQNGFQQSLYMTLEQVQE 
                   
               
               
                   
                   
                   
                   
                 HVSKYSKSGIDKDTKEFKGIWRNEFDTMAKKTVLKELLNRFGV 
                   
               
               
                   
                   
                   
                   
                 LSVEMQQAIEKDQADSEGRYIDNPQGGRYVQDAVIIEQSEPTEV 
                   
               
               
                   
                   
                   
                   
                 VSQEEPSPAPTKGIKQVDFKQM 
                   
               
               
                   
               
               
                 PF029 
                 SSAP 
                 recT 
                 
                   Salinisphaera hydrothermalis 
                 
                 MTNGASNQGRSGGQKKLIERIGDKYGVDANKMLSTLTATAFR 
                 166 
               
               
                   
                   
                   
                 C41B8 
                 QKNDQPITNEQMMALLVVADQYNLNPFTKEIYAFPDKNAGIVP 
                   
               
               
                   
                   
                   
                   
                 IVGVDGWSRIINEHDSEDGMDFEASDEFIELDKHHKKCPEYITC 
                   
               
               
                   
                   
                   
                   
                 RMYRKDRSRPVEITEYLDEVYRPPFMKNGNPMIGPWQSHTKRF 
                   
               
               
                   
                   
                   
                   
                 LRHKAMIQCARLAFGFVGIFDQDEAERIVQGEVVSSERNGAAP 
                   
               
               
                   
                   
                   
                   
                 PARQTEAPAAAERTLNDQQQNQVQAAIEESGADKAGLLKQLG 
                   
               
               
                   
                   
                   
                   
                 VASIDQIPVTRFSLVMDRINAQTEDA 
                   
               
               
                   
               
               
                 PF030 
                 SSAP 
                 recT 
                   Candidatus Cloacimonas  sp SDB 
                 MQSLAVAKNESLMPLSFQEKLSMASVFIKSNLMPRGYDTPEKV 
                 167 
               
               
                   
                   
                   
                   
                 VIALEMGHELGLPPLVAIMNIAIINGTPTLKADMMVALALRSGK 
                   
               
               
                   
                   
                   
                   
                 IEDIKIQYSGKENMNDNQFKCKVTIRRRGVETPFKASFSRQDAK 
                   
               
               
                   
                   
                   
                   
                 VAGLDYKDNWKKYERRMLKHRAMAFCIRDALPEIFAGVYLPE 
                   
               
               
                   
                   
                   
                   
                 ELEGIESYGGNRNTVILPAAQIEKTASNIEDNTADESLPSKVTDS 
                   
               
               
                   
                   
                   
                   
                 QIKALKELSERLFNEGIVTSYIYDQYAVNRIEDLDQGKAGKLIL 
                   
               
               
                   
                   
                   
                   
                 LLQNEPGKISEWIEDTYQKTA 
                   
               
               
                   
               
               
                 PF031 
                 SSAP 
                 recT 
                   Elusimicrobium minutum  (strain 
                 MTTENIQRTPALGILLEQAPFYSKAQNLEQKKIEDMMVSFAVIA 
                 168 
               
               
                   
                   
                   
                 Pei191) 
                 HKVIKRHQDKGRGEVDVQSIKEAFKCSMDTGIPVDNRRLAYLT 
                   
               
               
                   
                   
                   
                   
                 IVKNNSTGKYEIQYEPGYMGFVHKERQIKPGAVVQTILLWEKD 
                   
               
               
                   
                   
                   
                   
                 VFTYKSTTGVAEYSYVPEKPMRSDFNHIIGGFCVISYFEHGREY 
                   
               
               
                   
                   
                   
                   
                 SFVTPMTKAELDLARGKAKTQDVWGVWPGEMYKKVIIKRAG 
                   
               
               
                   
                   
                   
                   
                 KVEFIGEPEMEKLNEIDDRSYTGFERKQPAKVDYSAVKPLPQEI 
                   
               
               
                   
                   
                   
                   
                 TETEALPAPGGDDTVQDVFSMEEPQ 
                   
               
               
                   
               
               
                 PF033 
                 SSAP 
                 recT 
                   Spiroplasma kunkelii  CR2-3x 
                 MNNKIELIENNSKVNNWINEKLTNQKEINLFRKNILTIYNNNPN 
                 169 
               
               
                   
                   
                   
                   
                 LFDKEKINLMSVLSACVKAMLLDLPLDPNLGYAYIVPYNGKGQ 
                   
               
               
                   
                   
                   
                   
                 LQIGYKGYIQLAIRTGKYLTINAIEVKQGELLNFDELEQEYNFK 
                   
               
               
                   
                   
                   
                   
                 WITNENERTKKETIGYVAFFKLLNGYKKTLYWSKEKCENHFKT 
                   
               
               
                   
                   
                   
                   
                 YSKNYQTYGKFTVGSYEGMALKTVETQLLRKWGIMSVEMQE 
                   
               
               
                   
                   
                   
                   
                 VYQHDQAIIINNSKEFSDNPNIKNKEDKNVIELNNKKDELNLNL 
                   
               
               
                   
                   
                   
                   
                 EQEFSINEINDDEEIAKTVDEMFSD 
                   
               
               
                   
               
               
                 PF034 
                 SSAP 
                 recT 
                 
                   Avibacterium paragallinarum 
                 
                 MLKTAKPESIFNAACMAATLNLPLQNGLGFAYLVPFRTKRKVP 
                 170 
               
               
                   
                   
                   
                 JF4211 
                 LVDTEGNVILDRDGKPRTKEEYVIEAQFQIGYKGFIQLAQRSGQ 
                   
               
               
                   
                   
                   
                   
                 FKRLVALPVYKKQLVKKDFINGFEFDWEQEPEEGELPIGYYAY 
                   
               
               
                   
                   
                   
                   
                 FKLVNDFSAELYMTHEEIEKHAKKYSQTYRTYLEKKAKGQWA 
                   
               
               
                   
                   
                   
                   
                 SSVWADNFESMALKTVMKLLLSKQAPLSVEMQNAVLADQSVI 
                   
               
               
                   
                   
                   
                   
                 KDSENGEFDYPDNNIDDAEIVTMNVSQETFEQCKQNILNRETTL 
                   
               
               
                   
                   
                   
                   
                 QALCDSGFKFSPEQYAQLEALENNRE 
                   
               
               
                   
               
               
                 PF035 
                 SSAP 
                 recT 
                   Endozoicomonas montiporae , 
                 MPKATAKKSPATQVVDTAEKPATIKDLLRNNKNTIAQALENTP 
                 171 
               
               
                   
                   
                   
                   Endozoicomonas montiporae  CL- 
                 LTPERLLSVCMTEIRKTPKLRECSQASLVGSIIQSAQLGLEPGSA 
                   
               
               
                   
                   
                   
                 33 
                 LGHCYLIPFWNNKERSLECQFMLGYRGMLALARRSGSIVSIDA 
                   
               
               
                   
                   
                   
                   
                 RVVYAADEFSLLYGTITEIIHKPKETGEKGGVVGVYAVAQLQG 
                   
               
               
                   
                   
                   
                   
                 GGTQVDFMPVEDILKIRDSSKGVQSGKDTPWKTHFEEMAKKT 
                   
               
               
                   
                   
                   
                   
                 VVRRLFKFLPVSVEALTAVGLDEKAEAGQSQCNEALIQEGDGG 
                   
               
               
                   
                   
                   
                   
                 VLVDMDTGEIIEGKVTSAADLNRAL 
                   
               
               
                   
               
               
                 PF036 
                 SSAP 
                 recT 
                 
                   Roseateles depolymerans 
                 
                 MSEIAPVNQTAIAANTDLGNPSAFLFDSERMTALMEFSNLMSR 
                 172 
               
               
                   
                   
                   
                   
                 GSVTVPKHLQGKPADCMAITLQAMRWRMDPYIVGSKTHLVN 
                   
               
               
                   
                   
                   
                   
                 GNLGYEAQLVVAVLKNSGAVKGRPHYEFRGEGNNLECRAGFI 
                   
               
               
                   
                   
                   
                   
                 PAGEDAVLWTEWESISGIKVKNSPLWQTNPKQQFGYLQARNW 
                   
               
               
                   
                   
                   
                   
                 ARLYAPDALLGVYTEDELQVMPSQPSVRDMGQAEEVARPALP 
                   
               
               
                   
                   
                   
                   
                 AYPQADFDKNLPGWRNVVESGRKSAPDLLAMLQTKATFSAEQ 
                   
               
               
                   
                   
                   
                   
                 QEAILALGVIEQDAAEVDDPFVRDMEAAEGEQQ 
                   
               
               
                   
               
               
                 PF037 
                 SSAP 
                 recT 
                   Haemophilus parasuis  serovar 5 
                 MTTNLPSNIQTALSERGIDHAVWSTLQNSIFPGAKDESILLAIDY 
                 173 
               
               
                   
                   
                   
                 (strain SH0165) 
                 CKARKMDILKKPCHIVPMNVTNAKTAEKEWRDVIMPGIVEQRI 
                   
               
               
                   
                   
                   
                   
                 TAFRTGQMAGQDDPVFGDTIEYLGVNAPEWCKVTVYRFVNGE 
                   
               
               
                   
                   
                   
                   
                 RCAFSHTEYFTEACAITEIWKDKQRTGKYKVNSMWTKRPRGQ 
                   
               
               
                   
                   
                   
                   
                 LAKCAEAGALRKAFPDELGGVITADEITEEPINPASPQQTVIDV 
                   
               
               
                   
                   
                   
                   
                 DGVVRIADEQRQQLDQLIQITQTDVAKAIAVYGVNSLDQLPKD 
                   
               
               
                   
                   
                   
                   
                 KAEHLIKILNERVDKLQAQSENLGENIPL 
                   
               
               
                   
               
               
                 PF038 
                 SSAP 
                 recT 
                   Sodalis glossinidius  (strain 
                 MANELNTTSAILAEKGVDFATWSALKNSIYPGAKDESVMMAL 
                 174 
               
               
                   
                   
                   
                   morsitans ) 
                 DYCRARQLDPLMKPVHLVPMYITDAKTGKGQQRDIVMPGVEL 
                   
               
               
                   
                   
                   
                   
                 YRIQADRSGNYAGAKEPEFGPDETKIFGADETKNFKGIEVTFPQ 
                   
               
               
                   
                   
                   
                   
                 WCKYTVCKMMPSGQIVEYSAKEYWLENYATAGRDSSAPNAM 
                   
               
               
                   
                   
                   
                   
                 WKKRPYGQIAKCAEAQALRKAWPEIGQQPTAEEMEGKTEDVI 
                   
               
               
                   
                   
                   
                   
                 DARDVTPSASHSTSHKSATTETLQAINDLLLSLDKTWDEDFLPL 
                   
               
               
                   
                   
                   
                   
                 CSTVFKRQVSKAAELTDQEALKALDFLRKRATKDAA 
                   
               
               
                   
               
               
                 PF039 
                 SSAP 
                 recT 
                   Nocardia farcinica  (strain IFM 
                 MARNLVDRIEQNAPAKNDDLKAAIQKMEKAFALAMPKGAEA 
                 175 
               
               
                   
                   
                   
                 10152) 
                 TQLIRDAFTAIQRTPKLAQCEQLSVLGAFMTCAQLGLRPGVLG 
                   
               
               
                   
                   
                   
                   
                 QAYVLPFWDRKDRMYKAQFVAGYRGLVDLAYRSDRVLSVSA 
                   
               
               
                   
                   
                   
                   
                 RIVHANDYFELEYNAVEDRLVHRPYLDGARGEARLYVAAGRT 
                   
               
               
                   
                   
                   
                   
                 RGGGSAITDPVTVADMLKYRDRYATAKNKEGKVFGPWVDEF 
                   
               
               
                   
                   
                   
                   
                 DGMAKKTMIRQLSKMLPMSTELTLAVENDGSVRFDLGKDAIE 
                   
               
               
                   
                   
                   
                   
                 SPQRLEPDVIDAEAVATSDVQDAPADYVEDVPPAAEGGMFAPE 
                   
               
               
                   
                   
                   
                   
                 GE 
                   
               
               
                   
               
               
                 PF040 
                 SSAP 
                 recT 
                 
                   Parasutterella excrementihominis 
                 
                 MENIEIKAQTVSLVARLAAKFGVEPGKLMACLMNQVFKQSDG 
                 176 
               
               
                   
                   
                   
                 CAG:233 
                 VAPSNEELMVLLLVCENFGLNPFNREIFAFRGKGGDVIPVVSLD 
                   
               
               
                   
                   
                   
                   
                 GWCKIVRNQKDFNGMSFKFSESTIKLNCYGGELPEYVECSIKLK 
                   
               
               
                   
                   
                   
                   
                 GVEDPVTIQEYMVECFNEKSSVWRKWPRRMLRTRGFIQCARL 
                   
               
               
                   
                   
                   
                   
                 AFSLTGIYDEGDTFGEDSENGSGTTSDLKSQIPLVQQIPARPSLE 
                   
               
               
                   
                   
                   
                   
                 KSRLDALIGKLIEHAKNRDDGWKRAFQWIEEKFSQEDCAYARE 
                   
               
               
                   
                   
                   
                   
                 VLTAKRKELSLSSLPEGNEILPEETSPATLQGVSNEDR 
                   
               
               
                   
               
               
                 PF041 
                 SSAP 
                 recT 
                   Salinispora tropica  (strain ATCC 
                 MTQTVSQAVATRDNSPAGLITQYSESFAQVLPSHIKPATWVRL 
                 177 
               
               
                   
                   
                   
                 BAA-916/DSM 44818/CNB- 
                 AQGALKRGKRGDGGRFELEIAASNNPGVFLAALEDAARQGLE 
                   
               
               
                   
                   
                   
                 440) 
                 PGTEQYYLTPRKVKGRLEIEGITGYQGHIELMYRAGAVASVVA 
                   
               
               
                   
                   
                   
                   
                 EIVREHDEYRYQRGIDDVPVHRYKPFARDAERGALIGVYAYAR 
                   
               
               
                   
                   
                   
                   
                 MKDGAVSRVVELSRDDIDRIKASSQGANSEYSPWQKHEAAMW 
                   
               
               
                   
                   
                   
                   
                 LKSAVRQLQKWVPTSAEFRREQLRAAAEAHRVASAADAPDGA 
                   
               
               
                   
                   
                   
                   
                 TAPQGDVLDGEVLDEAPTEPARSDDAGGHVEQEWPDAARPGG 
                   
               
               
                   
                   
                   
                   
                 AQ 
                   
               
               
                   
               
               
                 PF042 
                 SSAP 
                 recT 
                 
                   Microbacterium ginsengisoli 
                 
                 MSEVALPSSVRPDTWNADTAAMMEFAGLTWIEGVGDAAHRV 
                 178 
               
               
                   
                   
                   
                   
                 FAPSGVMAAFIAACARTGLDPTAKQIYAAQMGGKWTVLVGID 
                   
               
               
                   
                   
                   
                   
                 GMRVVAQRTGQYDGQDPIEWLAAEDGQWTTVPPKAPYAARV 
                   
               
               
                   
                   
                   
                   
                 AIYRKGVSRPLVQTVTLAEFGGRGGNWSQRPSHMLGIRAESHA 
                   
               
               
                   
                   
                   
                   
                 FRRAFPMELAGLYTPEDFESDDVDTSDAPWEEREDWGALIASA 
                   
               
               
                   
                   
                   
                   
                 TTTGDLARVGARIAESGQGNDDIRAAYRARAAVLATEANTVD 
                   
               
               
                   
                   
                   
                   
                 ADVVDDQTPAEGEDASPTPPASPSAGTPDDYEAAASAEFDAAV 
                   
               
               
                   
                   
                   
                   
                 ERGEI 
                   
               
               
                   
               
               
                 PF043 
                 SSAP 
                 recT 
                 
                   Labilithrix luteola 
                 
                 MASTEMTRSAGAAPLARRGGGDLKEFLGSDAVRKKLAEAAG 
                 179 
               
               
                   
                   
                   
                   
                 KVMRPEDLIRFALVAASRTPDLAKCSKESVLRSLLDSAALNIMP 
                   
               
               
                   
                   
                   
                   
                 GGLMGRGYLVPRKNTKNNTTECHFDPGWRGLIDIARRGGKVR 
                   
               
               
                   
                   
                   
                   
                 RIEAHVVHAADVFSVERSPLTTLHHVPSELDDPGEIRAAYAVAE 
                   
               
               
                   
                   
                   
                   
                 FVDGGIQIEVVTRRDLNKIRAMGAKNGPWSTWGEEMARKTAV 
                   
               
               
                   
                   
                   
                   
                 RRLCKYLPYDPLLERAMHASDESDVNAFDEVVEVTAPKKRRR 
                   
               
               
                   
                   
                   
                   
                 SLDEKVDEVAASMLPPAEPDSQQDLPVDADFDEADDGTSDAA 
                   
               
               
                   
                   
                   
                   
                 EPTN 
                   
               
               
                   
               
               
                 PF044 
                 SSAP 
                 recT 
                   Agathobacter rectalis  (strain 
                 MAEQNAVATQQGTQLSVAAQVKSMISQDAVKKKFTEVLGQK 
                 180 
               
               
                   
                   
                   
                 ATCC 33656/DSM 3377/JCM 
                 APQFLASITNVVAGSAQLKKCPATTIMSAAFVAATYDLPIDSNL 
                   
               
               
                   
                   
                   
                 17463/KCTC5835/VPI 0990) 
                 GFAAIVPYNNNKYNPQTRQWEKHPEAQFQMMYKGFIQLAIRS 
                   
               
               
                   
                   
                   
                 ( Eubacterium rectale ) 
                 GYYEKMNCSVVYKDELVSYNPITGEVEFVTDFSKCTQRAEGKS 
                   
               
               
                   
                   
                   
                   
                 ENIAGYYAWFKLLTGFRKELFMTTAEVENHARKYSTAYRYDL 
                   
               
               
                   
                   
                   
                   
                 ENNKKGSKWTTDFEAMALKTVIKMLLSKWGILSVDMQRAIQD 
                   
               
               
                   
                   
                   
                   
                 DQKVYDEDGEGSYGDNQPDIVEAQDPFGNIEQKEEEQQIGGLD 
                   
               
               
                   
                   
                   
                   
                 LEEVE 
                   
               
               
                   
               
               
                 PF045 
                 SSAP 
                 recT 
                   Spiroplasma kunkelii  CR2-3x 
                 MITELQQVIKGAIIKYELKVNDKYLKENILALEELKMKNGQSYM 
                 181 
               
               
                   
                   
                   
                   
                 QLVNTADNTKITALGIIKLSNKGLIFGKDFNIIPFKNKLTTVIDS 
                   
               
               
                   
                   
                   
                   
                 KVYCKRIEESGYSPRKAIIFKGEKFEWDSENSCPKIHEINFNANT 
                   
               
               
                   
                   
                   
                   
                 SDYNEIIGAYAFAKDKNGNYQGILLRKADIERLKNSSPSGNSEY 
                   
               
               
                   
                   
                   
                   
                 SPWNKWPKEMVEAKLYRKLALEMGIDISDIDLDEKEIKEDGNF 
                   
               
               
                   
                   
                   
                   
                 EYISFKDINVAKNKGQISDEPLSSAFLIKILDIVFSLILAFSIVC 
                   
               
               
                   
                   
                   
                   
                 KISLYLFPSPKNEVILFIKFCSFLWYFFKPNSSSKG 
                   
               
               
                   
               
               
                 PF046 
                 SSAP 
                 recT 
                 
                   Methylobacterium nodulans 
                 
                 MTAGALTLSDAERRVAERVDPAATAALSVSAESGGVAFANAG 
                 182 
               
               
                   
                   
                   
                 (strain LMG 21967/CNCM I- 
                 QLMEFAKLMAVSSAGVRKHLRGNPGACLAICTQAVEWGMSP 
                   
               
               
                   
                   
                   
                 2342/ORS 2060) 
                 YAVANNSYFVNDQIAFESKLVQAVILKRAPIKGRIRFEYTGDGD 
                   
               
               
                   
                   
                   
                   
                 ERRCRAWARLADEPDEVVDYLSPPLGKITPKNSPLWKQDPDQ 
                   
               
               
                   
                   
                   
                   
                 QLAYFSGRALCRRHFPDVLLGVYADDEIEVAPRGPDQARDVTP 
                   
               
               
                   
                   
                   
                   
                 RGLAGRLDALAAAPPPTASAAVDAFTDTEAPGNGPATAPAEDE 
                   
               
               
                   
                   
                   
                   
                 PETGAQGADVGESGDWHDPALGGAADDFPGNDALSDMRTGR 
                   
               
               
                   
                   
                   
                   
                 RSSRWEA 
                   
               
               
                   
               
               
                 PF047 
                 SSAP 
                 recT 
                   Anaerococcus hydrogenalis  ACS- 
                 MTNAKNALKKNAQNKAPAKKQNTTVRGLLMAMKGEIQNALP 
                 183 
               
               
                   
                   
                   
                 025-V-Sch4 
                 SYLPTDKFIRTALTAINTTPKLAECTQDSLLAAIMNSAQLGLEEN 
                   
               
               
                   
                   
                   
                   
                 TPLGESYLIPYENKKLGITTVNFQIGYQGLLKLAYNTGQFKRIT 
                   
               
               
                   
                   
                   
                   
                 AREVRENEDFVINYGTGEVKHEPCLTGDSGDVIGYYAIYQTKD 
                   
               
               
                   
                   
                   
                   
                 GGQDVFYMSKADAEKYGRTYSKSYNFSSSPWQSNFDAMAKK 
                   
               
               
                   
                   
                   
                   
                 SCLIQVLKYAPKAIESQKLVEATKTDNANFKSYKKEDDGSINLD 
                   
               
               
                   
                   
                   
                   
                 VDYEVEVEEDKKEAPKNVDKETGEIKSEDIAQTGFFEDDFEPVS 
                   
               
               
                   
                   
                   
                   
                 E 
                   
               
               
                   
               
               
                 PF048 
                 SSAP 
                 recT 
                   Sporosarcina newyorkensis  2681 
                 MTNQLQPQSNTPAEKKNELVTKVADKVQRMVENNQINIPQNY 
                 184 
               
               
                   
                   
                   
                   
                 SIVNAVQAAYFKLTEVDFKKKTSLMDTATPDSVAFSLQDMAIQ 
                   
               
               
                   
                   
                   
                   
                 ALSVAKNQGYFIVYGDKMQFVRSYHGTQAVLKRLNGVKDVW 
                   
               
               
                   
                   
                   
                   
                 ANVIWKGETFDVEYNDRGQLAFKSHTVDWQAATGKKEDIQG 
                   
               
               
                   
                   
                   
                   
                 AYCIIEREDGVQFLTVMTMAEIKTSWSQSSTTAVQDKYPQEMA 
                   
               
               
                   
                   
                   
                   
                 KRTVINRASKAFINTSDDSDLFIGAVNRTTENEFEDDRPIKEINP 
                   
               
               
                   
                   
                   
                   
                 QHEIDQNANKEVLDFTEPEQSPQPVQEPELVQEPVRQPEPASSG 
                   
               
               
                   
                   
                   
                   
                 GPGF 
                   
               
               
                   
               
               
                 PF050 
                 SSAP 
                 recT 
                   Clostridium beijerinckii  (strain 
                 MAETGLVLSKEDAFNNVMAKIAALEKNNGIKLPKNYSAENAIN 
                 185 
               
               
                   
                   
                   
                 ATCC 51743/NCIMB 8052) 
                 SAWLMLQEVVDKEKKQALEVCTKTSIVEALYNMVLQGLSPSK 
                   
               
               
                   
                   
                   
                 ( Clostridium acetobutylicum ) 
                 RQCYFIVHGSKLTLMKSYMGSIVATKRLSGIKDVKAFVIYEGD 
                   
               
               
                   
                   
                   
                   
                 VFETVFNNETYTIEFNYQPKEENINSNKIKGAFALIIGDDNKLLH 
                   
               
               
                   
                   
                   
                   
                 TEIMTIDQIRKSWGMGIAYKSGKSNTHNDFGEEMAKKSVINRA 
                   
               
               
                   
                   
                   
                   
                 CKRFYNTSDDSDVLIESLNNTDEDYDEADIIENAKEQVHEEIKA 
                   
               
               
                   
                   
                   
                   
                 NANQEVIDVDPKQVTEIDNNNNENPIQKDLNNKKTEKAEQQM 
                   
               
               
                   
                   
                   
                   
                 MCEF 
                   
               
               
                   
               
               
                 PF051 
                 SSAP 
                 recT 
                   Paenibacillus alvei  DSM 29 
                 MSTAVQNINTQAVVGSFTQSELDTLKSTIAKGTSNEQFALFVQT 
                 186 
               
               
                   
                   
                   
                   
                 CARSGLNPFLNQIYCIVYNGKNGPVMSIQIAVEGIVALAKKHPQ 
                   
               
               
                   
                   
                   
                   
                 YKGFIAAEIRQNDHFKAKIHTGEVEHEPDVMNPGETIGAYCVA 
                   
               
               
                   
                   
                   
                   
                 YRENAPNVLVIVRRDQVEHLLKGRNSDMWRDYFDDMIVKHAI 
                   
               
               
                   
                   
                   
                   
                 KRAFKRQFGIEVSEDEYVQPNSIDNTASYETRKDITADVESGTP 
                   
               
               
                   
                   
                   
                   
                 QLQQPLQNSQNGENGEIKKVRRDISAAFKKLGITTEKAMTEYT 
                   
               
               
                   
                   
                   
                   
                 MARMKQKGDKPTLQELTGLLKVMQLEIEEKEVFADGASEAGL 
                   
               
               
                   
                   
                   
                   
                 EPLE 
                   
               
               
                   
               
               
                 PF052 
                 SSAP 
                 recT 
                 
                   Bacillus sporothermodurans 
                 
                 MANNQLAVYQDLTFGELTQQDIVTVRETIGKDCNESQFKLFMS 
                 187 
               
               
                   
                   
                   
                   
                 IAKNSGANPILNEIYPAVRGGQLTVQFGIDFYVRKAKETEGYQG 
                   
               
               
                   
                   
                   
                   
                 YDVQLVHENDGFKMHQEKDEDGRYVIVIDEHSWGFPRGKVIG 
                   
               
               
                   
                   
                   
                   
                 GYAIAYKEGLKPFTVIMEVDEVEHFKKSNIGMQKTMWTNYFN 
                   
               
               
                   
                   
                   
                   
                 DMFKKHMTRRALKAAFNLNFDDDEVGEGSGSDGIPEYKPQTR 
                   
               
               
                   
                   
                   
                   
                 KDITPNQEIIDAPSKQEVHEEDPQLAQAKKDMKTKFKKLGITTK 
                   
               
               
                   
                   
                   
                   
                 KGMQDYIKQHAPQIGDNPTYQQLVGLLDIMDMHIDMNEVQAS 
                   
               
               
                   
                   
                   
                   
                 DADDVLE 
                   
               
               
                   
               
               
                 PF053 
                 SSAP 
                 recT 
                   Bacillus  sp 2_A_57_CT2 
                 MAKNNELATQSAELNELHQIGGFGVNELMTMKETVGKDLSIP 
                 188 
               
               
                   
                   
                   
                   
                 QFNLFMYQCNRMGLDPSLRHAFPIVYGGKMDIRVSYEGLKSLA 
                   
               
               
                   
                   
                   
                   
                 QKSDGYQGVFTQVVCENEIDDFDVLLNDEGEMVGVKHKPRFP 
                   
               
               
                   
                   
                   
                   
                 RGKVLGAYAVAKREGRSNYVVFMDVSEVQKWMKINGKFWK 
                   
               
               
                   
                   
                   
                   
                 QDNGDVDPDMFKKHVGTRAIKGQFDIADVVVDGMEAVADSN 
                   
               
               
                   
                   
                   
                   
                 PIPEYKPGERKDITPDNNVIEPPKESEEEKHKKAERSSINQAFKK 
                   
               
               
                   
                   
                   
                   
                 LGITGKKETAEYIEKHAPSFTNENPSEADLNGLLKLLEMNLEMI 
                   
               
               
                   
                   
                   
                   
                 EMQSGGDELE 
                   
               
               
                   
               
               
                 PF054 
                 SSAP 
                 recT 
                 
                   Thermaerobacter marianensis 
                 
                 MAVQTDQVRNKLARRAQENGAPAPSQQPKTIEQWLRDERFRA 
                 189 
               
               
                   
                   
                   
                 (strain ATCC 700841/DSM 
                 EIERALPRHLSADRKKRITLTVLRTTPELRRCTVPSLLAAVLQCA 
                   
               
               
                   
                   
                   
                 12885/JCM10246/7p75a) 
                 QLGLEPGVLGHVYLVPFKNGKTGEYEVQVIIGYKGWVELARR 
                   
               
               
                   
                   
                   
                   
                 SGQIQSLTARVVYQNDEFELSFGIEDNERHVPWYMRPNVQDGG 
                   
               
               
                   
                   
                   
                   
                 PIRGAYSVARFKDGGYHLHYMPIQQIEARRKRSRAADSGPWKT 
                   
               
               
                   
                   
                   
                   
                 DYEAMVLKTVVRDASKWWPLSPEIARGLAQDESIKRTVDDVD 
                   
               
               
                   
                   
                   
                   
                 SDAPYFGEDVIDVQGEDVGEGGEDAAHEEGGDASAGGDGSAQ 
                   
               
               
                   
                   
                   
                   
                 FGLFGGGGQ 
                   
               
               
                   
               
               
                 PF055 
                 SSAP 
                 recT 
                 
                   Salinicoccus halodurans 
                 
                 MTKNEVLVKNQKMGDNVLARVKELEGQGNLNFPANYAPENA 
                 190 
               
               
                   
                   
                   
                   
                 MKSAMLILQDLKGSKKDGYKPALEFANPNSVANALMDMVVQ 
                   
               
               
                   
                   
                   
                   
                 GLNPQKSQGYFIMYGDKVQFQRSYLGTMSVTKRVTGAKEINA 
                   
               
               
                   
                   
                   
                   
                 EVIFEGDDVEYETINGKITNLKHKTKFGNRDTKNIIGAVATIVFE 
                   
               
               
                   
                   
                   
                   
                 DESKNYTEIMTTDEIETAWKQSQMVYNGEFKADGTHRKYPQE 
                   
               
               
                   
                   
                   
                   
                 MSKKTVINRACKKLLNSSDDSSLLKQQVMNSDDRQRKEVFDT 
                   
               
               
                   
                   
                   
                   
                 QVSENHATEDLDFPDDVVDGEFKEADQEPEPMQKPVDYDEET 
                   
               
               
                   
                   
                   
                   
                 GEVKEDDKDDNPF 
                   
               
               
                   
               
               
                 PF056 
                 SSAP 
                 recT 
                   Dialister  sp CAG:486 
                 MDARKGIVATRNEMTAQQQERPSIPKLLNNTLDNSGYKRRFDE 
                 191 
               
               
                   
                   
                   
                   
                 LLGKRAPQFVSSLAALINSTPQVLSIFQNNPVAIIQSALKAAAVD 
                   
               
               
                   
                   
                   
                   
                 LPIEPSEGYAYILPFGQTATFVLGYKGMVQLAERTGLYMRLNA 
                   
               
               
                   
                   
                   
                   
                 VDVREGELISYDRLTEDIEFRWEADNAKRAKIPVIGYAAFYRLK 
                   
               
               
                   
                   
                   
                   
                 NGMEKTLFMTKAEIDAHELANRKGRSQNPVWRNHYDEMAKK 
                   
               
               
                   
                   
                   
                   
                 TVLRRLLSKWGIMSINYLDASPADQEVMRNMAEGTLDDTEMP 
                   
               
               
                   
                   
                   
                   
                 QERTIANSPNLAPVSPADPAEAITIPWEQGNAQEGQNQAVAGE 
                   
               
               
                   
                   
                   
                   
                 AMNGGEGK 
                   
               
               
                   
               
               
                 PF057 
                 SSAP 
                 recT 
                   Leuconostoc mesenteroides  subsp 
                 MANEVAQVQKIINSDKMQKHFEEILQDNAAGFLSGLSTVVALN 
                 192 
               
               
                   
                   
                   
                 mesenteroides (strain ATCC 
                 PDLAKTNMNDLTNAAMRAAILDLSVLPDLGEAYVIPYGKRAK 
                   
               
               
                   
                   
                   
                 8293/NCDO 523) 
                 VDGKWVTKDVKAQFQLGYRGIIKLVQNTGRVGRLGGSVVYE 
                   
               
               
                   
                   
                   
                   
                 ANKPHYNYVFDEFTMENENYDPYVDGESPVAGYLAFYYLDGE 
                   
               
               
                   
                   
                   
                   
                 RIVKYWPIQRVINHATKFSQTYKGPDHKDRYGKTPQTPWYTDF 
                   
               
               
                   
                   
                   
                   
                 DAMAIKTVMKDLLKFAPKTTKVAQAIAEDDKNEREARDVTPE 
                   
               
               
                   
                   
                   
                   
                 TEEITPEEQNVEPEIIDNQPAEKSDNPFSGVDTGDAPNPFAEKNE 
                   
               
               
                   
                   
                   
                   
                 TSEDVKWVSQKQ 
                   
               
               
                   
               
               
                 PF058 
                 SSAP 
                 recT 
                 gamma proteobacterium BDW918 
                 MSDQNPLVICKDQIAKSAVKFGSSELSYESEEIFAMQQLMKND 
                 193 
               
               
                   
                   
                   
                   
                 YLLKTAVNDPDSLRLAMYNVASTGISLNPARHEAYLVPRADK 
                   
               
               
                   
                   
                   
                   
                 KGQPAKIKLDISYRGLIALAQSEGIIANAVCELVYEQDTYLFKG 
                   
               
               
                   
                   
                   
                   
                 PTRIPEHAANVFSTTRGAVIGGYCITELTNGKVQIHNMSKADM 
                   
               
               
                   
                   
                   
                   
                 DQIRDSSQAFGSGSGPWVEWESQMQLKSIVKRAAKWWPASTP 
                   
               
               
                   
                   
                   
                   
                 KMAKVIEFLNVENGEGIATIEHQPSNRIVAPAKPEDIDSRTLGFI 
                   
               
               
                   
                   
                   
                   
                 NQALTRAVQSQSFEACGELIRERVKDPAALAYGLEKLKELQTQ 
                   
               
               
                   
                   
                   
                   
                 HGDYRHAG 
                   
               
               
                   
               
               
                 PF059 
                 SSAP 
                 recT 
                   Paenibacillus  sp P1XP2 
                 MANNTQIILTPEIKEAFPAEVLDVIRTSLCPTATDPEFLLFAHKA 
                 194 
               
               
                   
                   
                   
                   
                 ASYRLDPFKNEIFFIKYGSQARIQFAAEAYLAKAREKEGFQPPD 
                   
               
               
                   
                   
                   
                   
                 TQTVCANDTFKARKFKNDKGEDEWEVIEHEVTFPRGPIVGAYS 
                   
               
               
                   
                   
                   
                   
                 IAYRDGYRPVTVFVDKEHIAHMYTGQNKDNWNKWEPDMIGK 
                   
               
               
                   
                   
                   
                   
                 HAEQRALKKQYGLDFGNEELEHPPAPAASSFERKDITNEVNQA 
                   
               
               
                   
                   
                   
                   
                 TAAANAQSTPSSDQPNGSQAEEDEEAKINALKAQMKQNFAKL 
                   
               
               
                   
                   
                   
                   
                 GLVNKADRDAHMAKHFKIKGEQPTIAEIMAYLKIMDLQIQEKQ 
                   
               
               
                   
                   
                   
                   
                 ASLNDELPL 
                   
               
               
                   
               
               
                 PF061 
                 SSAP 
                 recT 
                   Gramella forsetii  (strain KT0803) 
                 MSSETSKQLVKQKKQDISTRVLAKVNEFEQTGELRIPKNYSVE 
                 195 
               
               
                   
                   
                   
                   
                 NSLKSAYLIELSETKDKNKKPVLESCSVTSLSEALLKMVVWGLN 
                   
               
               
                   
                   
                   
                   
                 PMKKQCYFVPYGGKIECIPDYTGKIAMAKRYAGLKDIKAHAVF 
                   
               
               
                   
                   
                   
                   
                 KDDTFEFEVDPSTGRKKVTKHTQTLESMGSNEFKGAYAIMEM 
                   
               
               
                   
                   
                   
                   
                 NDGTFDVEIMSKPQIVAAWQQGHANGTSPAHKKFPDRMARKS 
                   
               
               
                   
                   
                   
                   
                 VINRACDALIRSSDDSVLYEDEDERKIIDVPSEDLKHEVKTKAN 
                   
               
               
                   
                   
                   
                   
                 KRNFDTSDIEDADYEEDEEPTNEAENEDDENERLYEEAMAEEE 
                   
               
               
                   
                   
                   
                   
                 GQSSMANSPGFGA 
                   
               
               
                   
               
               
                 PF062 
                 SSAP 
                 recT 
                   Brevibacillus brevis  (strain 47/ 
                 MSEAKTVNQSALAGQLAQRTMTKAENFNAVIKKELADNFQAI 
                 196 
               
               
                   
                   
                   
                 JCM 6285/NBRC 100599) 
                 KSLVPKHMTPERLARITLTAISRTPALIDCTPASIVGAVMNCATL 
                   
               
               
                   
                   
                   
                   
                 GLEPNLIGHAYLVPFKNNRTKQMECQFQIGYKGQIDLIRRTGD 
                   
               
               
                   
                   
                   
                   
                 VSKIYAETVYENDLFIYIKGEDKRLVHVPFDMLHHLENFTPSKD 
                   
               
               
                   
                   
                   
                   
                 DFMDIMMAQAIGAIKSRGANDQGKPVRYYSAYRLKDGAFDFV 
                   
               
               
                   
                   
                   
                   
                 TMTAEQCQKHAMTHSAAKKDGKLVGPWKDHFESMCKKTCIK 
                   
               
               
                   
                   
                   
                   
                 EMAKYMPISIEVQEKLSTDEAVLKLRKDNGIESDNIFDVDYKIV 
                   
               
               
                   
                   
                   
                   
                 EEGQAEPDEEAAE 
                   
               
               
                   
               
               
                 PF063 
                 SSAP 
                 recT 
                   Prevotella  sp CAG:873 
                 MSKVNFSAQYIASLKPMQIVKDNLVRQRFIDLYGALWGEANA 
                 197 
               
               
                   
                   
                   
                   
                 EATYEREVIHFNRLLADNANLKACTPVSIFIAFIDLAVCGLSVEP 
                   
               
               
                   
                   
                   
                   
                 GVRALAYLQPRGYKTGQKDATDKDIYEQRCTLTISGYGELIQR 
                   
               
               
                   
                   
                   
                   
                 TRAGQIRHADNPVIVYEGDGFSFTDHNGTKSVEYTLNINHNPER 
                   
               
               
                   
                   
                   
                   
                 PVACFMRITRADGSIDYAVMLEEDWRRLAGYSGKANKKWDY 
                   
               
               
                   
                   
                   
                   
                 DTRSYVEVPNSLYSSGEGNRIDSGFLMAKCIKHAFKTYPKLRIG 
                   
               
               
                   
                   
                   
                   
                 KGTTYEADEAPRQEDDFYGMGDNEQPTAQPEESFAPAPDTSAG 
                   
               
               
                   
                   
                   
                   
                 VTVDPSQSDDNDETF 
                   
               
               
                   
               
               
                 PF064 
                 SSAP 
                 recT 
                   Bacillus  sp 1NLA3E 
                 MSTQNELAVKTSSERFLNNIQAQFAAEAGSPVAFSDYEKALAQ 
                 198 
               
               
                   
                   
                   
                   
                 HLFLKLDSVLQDFEAKRINSGKTQQAPYNWHNINMRKLSLDA 
                   
               
               
                   
                   
                   
                   
                 VHTVQLGLDALIANHIHPVPYFNGKEKKYDLDLRVGYIGKAYY 
                   
               
               
                   
                   
                   
                   
                 RMEAAVEKPKDVVIELVYSTDHFKPMKKSFSNSIESYEFEIQKP 
                   
               
               
                   
                   
                   
                   
                 FDRGEIVGGFGYLVYDDPAKNKLILVSEADFDKSRKAAKGDTF 
                   
               
               
                   
                   
                   
                   
                 WSKHPAEMRFKTLVHRVTEKLQIDPKKVNASYLYVENKESED 
                   
               
               
                   
                   
                   
                   
                 EVRKEISENANKDVIDVEFSETPDEPVNKEPKVENHSEHFEEAP 
                   
               
               
                   
                   
                   
                   
                 PQQEQMEFAPTGTGGPGF 
                   
               
               
                   
               
               
                 PF065 
                 SSAP 
                 recT 
                   Faecalibacterium  sp CAG:82 
                 MAKAMQPQKLYFSQAMQTEKYKKLINNTLGDPVRAARFAANI 
                 199 
               
               
                   
                   
                   
                   
                 TSAVAVNPTLQECDAGTILAGALLGESELLQPSPQLGQFYLVPF 
                   
               
               
                   
                   
                   
                   
                 KSKAKRDRQGNVIEPACLKAQFVLGYKGYTQLALRTGQYKRL 
                   
               
               
                   
                   
                   
                   
                 NVLEVKSGELGGWDPFEEREHEMHFIEDFEKRAAMPTVGYIAH 
                   
               
               
                   
                   
                   
                   
                 FEYINGFEKTLYWTADRMMAHADKVSPAFSATAYKKLLNGEI 
                   
               
               
                   
                   
                   
                   
                 PQEDMWKYSSFWYRDFDGMAKKTMLRQLISKWGIMTVEMTT 
                   
               
               
                   
                   
                   
                   
                 AYERDGRVMVPNSADDGLLPETPDFADAGQNGLSEQDPPKIER 
                   
               
               
                   
                   
                   
                   
                 TAKTMDLPEPEADEVKAAVDLATL 
                   
               
               
                   
               
               
                 PF066 
                 SSAP 
                 recT 
                   Pelobacter propionicus  (strain 
                 MSNALVKLEFDKEQMAVIETQLEPSGTSKAEQQVCLSVARELC 
                 200 
               
               
                   
                   
                   
                 DSM 2379/NBRC 103807/ 
                 LNPITKEIFFVKRRQKIDDKWVTKVEPMVGRDGFLSIAHRSKQF 
                   
               
               
                   
                   
                   
                 OttBd1) 
                 AGIETTAGIREVPQLEGGQWGFKNQLVTECIVWRKDSPKPFTV 
                   
               
               
                   
                   
                   
                   
                 QVAYNEYCQRNSEGNPTKFWAEKPETMLKKVAESQALRKAFN 
                   
               
               
                   
                   
                   
                   
                 IHGVYCPEELGAGFELASGDIVIQAIEEERPGNETDKSHLSVVKP 
                   
               
               
                   
                   
                   
                   
                 PQAETQATKTPKHQKSSTATTSQSAPINEEVQTSPPSPGQVIDEA 
                   
               
               
                   
                   
                   
                   
                 ALEVIELLDGKHIPYDIAINGEDGIISAKSFNEKELEKSSGFRWS 
                   
               
               
                   
                   
                   
                   
                 ADQKRWIYKFRNEPF 
                   
               
               
                   
               
               
                 PF067 
                 SSAP 
                 recT 
                   Herbaspirillum  sp YR522 
                 MNQVALSPAGTLNQFLKQHKNQIEMALPKHITPDRMMRLTLT 
                 201 
               
               
                   
                   
                   
                   
                 AFSQNRSLQDCTPQSIFASVIVASQLGLEIGVGGQGYLVPYKGT 
                   
               
               
                   
                   
                   
                   
                 CTFVPGWQGLVDLVSRAGRATVWTGAVYRGDKFDWALGDRP 
                   
               
               
                   
                   
                   
                   
                 FVKHQPEGDGDDWHDITHVYAIGRVNGSDHPVIEVWTMDRIV 
                   
               
               
                   
                   
                   
                   
                 RHLNKFNKVGGKHYALTNNGQNMEMYARKVALLQVLKYMP 
                   
               
               
                   
                   
                   
                   
                 KSVEVMRAMDVANAVDSGKNFTFDGDVVVIDDRDIDESPGDS 
                   
               
               
                   
                   
                   
                   
                 SPGATGVQQQSGGRPEIPECTDEEFKAKTPRWRKQLLEDGVSE 
                   
               
               
                   
                   
                   
                   
                 ADLVKMIETRTKLTEDQKTTINSWTHEND 
                   
               
               
                   
               
               
                 PF068 
                 SSAP 
                 recT 
                   Desulfovibrio  sp FW1012B 
                 MSNGNLPQESGGASVAALIQQQIPAIAMAVSGGTKEERQKRAE 
                 202 
               
               
                   
                   
                   
                   
                 RFARVALTTIRNNDKLAQCRVESLLGALMTSASLNLEIDPRGLA 
                   
               
               
                   
                   
                   
                   
                 YLIPYGREAQLQIGYKGIKELAYRAGGIKAIYAEVVYKPEVEAG 
                   
               
               
                   
                   
                   
                   
                 MFTIEIGLSRSLTHKLDPERPELRNGDLVLAYAVAEMEDGRRH 
                   
               
               
                   
                   
                   
                   
                 FAYCLRDEVEKRRKTSKMNTASPDSTWGKWAEEMWRKTAVK 
                   
               
               
                   
                   
                   
                   
                 KLCKDLPQSTEDAMAKAVALDDQAEAGVPQTFDLPKDFIDVT 
                   
               
               
                   
                   
                   
                   
                 PEPKTASDRAAQVLGKTEAEAAPPVDAPTSVPCPNRPNDETGG 
                   
               
               
                   
                   
                   
                   
                 FWDVKATVCEGCMDRGGCPSWVAA 
                   
               
               
                   
               
               
                 PF069 
                 SSAP 
                 recT 
                   Nitrolancea hollandica  Lb 
                 MADNNHALAIVPDSNEVKSLAKRLVPQFPSDCNAEQAADVAR 
                 203 
               
               
                   
                   
                   
                   
                 VAVAYGLDPFLGELIPYKGKPYLTFDGRIRIADNHPAYDGYDH 
                   
               
               
                   
                   
                   
                   
                 GPVLGDERTAFMPQNGEVIWKCTVYRRDRSRPTVAYGRAGGD 
                   
               
               
                   
                   
                   
                   
                 KETNPIAKKDPVTMAQKRAIHRALRAAFPVPIPGGDDDPVTPA 
                   
               
               
                   
                   
                   
                   
                 QLKAIHATDSEQGITVTERHEVLEATFGVGSSKDLTAAQAGAY 
                   
               
               
                   
                   
                   
                   
                 LDERAAQKPAMEQPAIEVTARPATPPQEPTDPPVGALLSARQQ 
                   
               
               
                   
                   
                   
                   
                 RRIYELRDELGKQTAELNAAIQELYKVDSIEGLSEAQASRFIRSL 
                   
               
               
                   
                   
                   
                   
                 QRSAEKARQQQADDVIDAELADIPF 
                   
               
               
                   
               
               
                 PF070 
                 SSAP 
                 recT 
                   Fusobacterium mortiferum  ATCC 
                 MAETKRATNSLVKGSNGAVTEKKPNKTIYEIIKAGEKQFAAAL 
                 204 
               
               
                   
                   
                   
                 9817 
                 PKHLNSERFTRIAITTIRQNPKLAECNAESLLGSLMTIAQLGLEP 
                   
               
               
                   
                   
                   
                   
                 GVLGQCYLIPFKNNKLGTIECQFQLGYKGMIELLRRTGQLSDIY 
                   
               
               
                   
                   
                   
                   
                 AYTVYSNDEFDIEYGLDRTLKHKPAFTNPEGRGEIVGFYSVAIL 
                   
               
               
                   
                   
                   
                   
                 KDGTRAFEYMTKKEVIEHEEKYRKGNFKNEIWNKNFEEMSLK 
                   
               
               
                   
                   
                   
                   
                 TVTKKMLKWLPISVEMIENLRKDEQIHKLDEKTNEVTSEYIDEN 
                   
               
               
                   
                   
                   
                   
                 IINYDEDGVIVEEKPTTEDMQTEMTISQASGIDITKKAKELYGVS 
                   
               
               
                   
                   
                   
                   
                 TLDDINMEQYNELKEIAING 
                   
               
               
                   
               
               
                 PF071 
                 SSAP 
                 recT 
                   Akkermansia  sp KLE1798 
                 MALTPGLVTLNHAKIMSNESTDKLDLPQAPVPKKTLYEIVMSE 
                 205 
               
               
                   
                   
                   
                   
                 DVKTHITQFVEGMMTPERCISIFWNCCQKTPLLQQCAPITLISSL 
                   
               
               
                   
                   
                   
                   
                 KNLLLMRCEPDGIHGYLVPFWVNDKRTGNSILTCAPVPSARGL 
                   
               
               
                   
                   
                   
                   
                 MRMARSNGVTNLNIGIVREGEPFSWREDDGKFTMGHIPGWGD 
                   
               
               
                   
                   
                   
                   
                 NEDPIRGFYCTWTDKDSYLHGERMSLKAVEEIKGRSKSKNKKG 
                   
               
               
                   
                   
                   
                   
                 EIVGPWVTDFGQMGLKTVIKRASKQWDLPLVIQAAMQAADDQ 
                   
               
               
                   
                   
                   
                   
                 EFEGNMRNVTPEKTDGPAEGETPWNNAPAPEAFQNDQPEALPE 
                   
               
               
                   
                   
                   
                   
                 PKPEGQDDLIPGLKMPAPKETATVNMEDY 
                   
               
               
                   
               
               
                 PF072 
                 SSAP 
                 recT 
                   Thiorhodovibrio  sp 970 
                 MTRSMQAVPKADAPRIPMPAVDPALNLTPSTWKVLTDSIEFPAA 
                 206 
               
               
                   
                   
                   
                   
                 KTAEGILLAVHYCAARNLDVIKRPVHVVPMWSKALGQEIETV 
                   
               
               
                   
                   
                   
                   
                 WPGIAEVQTTAARTGQWAGIDPPRFGPVIERTFSGKVKRNGAW 
                   
               
               
                   
                   
                   
                   
                 QDLDYAVSFPEWCEVTVYRLVGGQRCPFTEPVFWLEAYARQG 
                   
               
               
                   
                   
                   
                   
                 GAYSELPTEMWVKRPRGQLMKCAKAASLRAAFPEEASYTAEE 
                   
               
               
                   
                   
                   
                   
                 MEGKVIEADTSIPVAASLDATGTVPAKADTAPSGVAEAASDAA 
                   
               
               
                   
                   
                   
                   
                 PSKASETPTDSEPRAEPITLDPSLQARIDKVVARAAEKSAWKQA 
                   
               
               
                   
                   
                   
                   
                 EQYLRARCKGAELKQALEALDQAQQTAEQRLAA 
                   
               
               
                   
               
               
                 PF073 
                 SSAP 
                 recT 
                   Pedobacter antarcticus , 
                 MSNQIQVTKDYIDRLHPLSVVKDAAIGDHFINKFVAMYRVPRE 
                 207 
               
               
                   
                   
                   
                   Pedobacter   antarcticus  4BY 
                 QAVAFHEREKDNFIKRITDSEDLSACTPMSIFLAYMQVGGWQL 
                   
               
               
                   
                   
                   
                   
                 SFEGGPQSDVYLIPGNRNVAPKGQPDKWIKEVVAQPTPYGEKK 
                   
               
               
                   
                   
                   
                   
                 IRIQNGQIKDAAKPIIVYECDDYEEFTDDIGNVRVTWKKGNRGD 
                   
               
               
                   
                   
                   
                   
                 KPVIVGSFIRIEKPDGSFEIKTFDMGDVAKWKASSENKNSKWD 
                   
               
               
                   
                   
                   
                   
                 AAAGRKLPGKANALYTSNSGQIDKLFFEGKTLKHAFKLYPKVV 
                   
               
               
                   
                   
                   
                   
                 NSPKLPDAFVPVASDAIRQGFDVSEFTEAEYVPEEDLSQSEQSD 
                   
               
               
                   
                   
                   
                   
                 FDVALEEAHNTEPIQTKTFAGIDTSDEPEF 
                   
               
               
                   
               
               
                 PF074 
                 SSAP 
                 recT 
                   Rhizobium loti  (strain 
                 MMNAITTYTHSPRQLALIQKTVAKDCNTDEFNLFVEVARAKGL 
                 208 
               
               
                   
                   
                   
                 MAFF303099) ( Mesorhizobium   
                 DPFLGQIIPMIFSKGDSNKRKMTIIISRDGQRVIAQRCGDYRPAS 
                   
               
               
                   
                   
                   
                   loti ) 
                 KPPSYEFDAELKSETNPQGIVSATVYLWKQDAKTAAWFEVAG 
                   
               
               
                   
                   
                   
                   
                 QSYWDEFAPISYPYDAYKMVDTGETWEDSGKPKKKRVLRDG 
                   
               
               
                   
                   
                   
                   
                 ATPQLDDSGNWCRMPRLMIAKCAEMQALRAGWPEQFTGLYD 
                   
               
               
                   
                   
                   
                   
                 EAEMDRAKVLEMAASEIVAHEQEENRLRLVAGNDAITMSWGD 
                   
               
               
                   
                   
                   
                   
                 GWALENVPAGEFMDRCLAFIRESDHQTVVKWNSANRAGLQLF 
                   
               
               
                   
                   
                   
                   
                 WAKHPGDALELKKAIEKASRAPVEIDHIADAARREIAQHPVSA 
                   
               
               
                   
                   
                   
                   
                 G 
                   
               
               
                   
               
               
                 PF075 
                 SSAP 
                 recT 
                   Stigmatella aurantiaca  (strain 
                 MDGHNKAETTAAQQAWGRERVELIKRTICPKGIGDDEFSLFIE 
                 209 
               
               
                   
                   
                   
                 DW4/3-1) 
                 QCKRSGLDPLLKEAFCVGRRQNAGSRERPTWVTRYEFQPSEAG 
                   
               
               
                   
                   
                   
                   
                 MLARAERFPDFKGIQASAVYAEDEIIVDQGKGEVVHRFNPAKR 
                   
               
               
                   
                   
                   
                   
                 KGALVGAWARVVREGKEPVVVWLDFSGYVQQTPLWAKIPTT 
                   
               
               
                   
                   
                   
                   
                 MIEKCARVAALRKAYPEAFGGLYVREEMPAEEYEPSSAAEEPA 
                   
               
               
                   
                   
                   
                   
                 PTTGTGTYEVLGARPGPVKASFPPLPAAQLSMEVQPPVAAPVA 
                   
               
               
                   
                   
                   
                   
                 EPPPAAETAPRPRSSATVVAFGPYKGKTASELSDDELSETIDLA 
                   
               
               
                   
                   
                   
                   
                 HEKLMEQPKAKWAKAMRENLVALDAETELRCRVPASGKKEA 
                   
               
               
                   
                   
                   
                   
                 SAEA 
                   
               
               
                   
               
               
                 PF076 
                 SSAP 
                 recT 
                 
                   Methyloversatilis universalis 
                 
                 MTGPELAAAAKQTAQQAGSATVKKFFEANRGTLEALLPRHFD 
                 210 
               
               
                   
                   
                   
                 (strain ATCC BAA-1314/JCM 
                 SERMLKLALGALRTTPKLANASLSSLLGSVVTCAQLGLEPNTPL 
                   
               
               
                   
                   
                   
                 13912/FAM5) 
                 GHAYLLPFDKREKQNGQWVTVETQVQVIIGYKGMLDLARRSG 
                   
               
               
                   
                   
                   
                   
                 QIVSIAAHEVCQNDEFVFAYGLNEELVHRPAMKDRGPVIGFYA 
                   
               
               
                   
                   
                   
                   
                 VAKLTGGGYSFEFMSVDEVNHIRDKAAEKNRAKRDAAGNPIIT 
                   
               
               
                   
                   
                   
                   
                 GPWADNYVEMGRKTVLRRLFKYLPISIESLAFASAVDGQAIREP 
                   
               
               
                   
                   
                   
                   
                 APLEQVAFESSEPAEEPTSYDQQDEDLRALEQSQPARVPPVQVE 
                   
               
               
                   
                   
                   
                   
                 QPAEAWQPSAEEAAELERQQAAEAAADQQRASAPRGRGQRS 
                   
               
               
                   
                   
                   
                   
                 MSLE 
                   
               
               
                   
               
               
                 PF077 
                 SSAP 
                 recT 
                 
                   Hydrogenovibrio marinus 
                 
                 MYSQQAYPEQNQYPAATNNVVSVFDNSENKSAIKKLLPRHISI 
                 211 
               
               
                   
                   
                   
                   
                 DKEMQIANTAAMQFPDLQECTPESLFVAFSRCAQDGLIPDGRE 
                   
               
               
                   
                   
                   
                   
                 AAIVSYNKKQGNTWIKVAQYQPMVEGVLKRLRMSSQVKNVIA 
                   
               
               
                   
                   
                   
                   
                 KVVYENENFNHWIDIDGEHLMHQPVFDDKRGELKLVYALAKL 
                   
               
               
                   
                   
                   
                   
                 DSGEKVVEVMLKSEVEEIMMNSKSAVDKNGVLKPYSVWATH 
                   
               
               
                   
                   
                   
                   
                 FPRMALKTVIHRITRRLPNASEVAEMLEREIDYKEVSEDRQPQT 
                   
               
               
                   
                   
                   
                   
                 IEHNQNQGSQDREIIPLEQQMELKKLVEQTGSDENNMFNWISN 
                   
               
               
                   
                   
                   
                   
                 KTEILVSSYDQLDFNQFTRLKTRLENAIAKQVAAQRQQAMEQS 
                   
               
               
                   
                   
                   
                   
                 GEFVSA 
                   
               
               
                   
               
               
                 PF078 
                 SSAP 
                 recT 
                   Photobacterium profundum  (strain 
                 MNELQRTNNQAPVNHQVNAVPTSSISILDTNKLDVMIRAAEFM 
                 212 
               
               
                   
                   
                   
                 SS9) 
                 SKAVVTVPEHLRGNSSDCLAIVMQAEQWGMNPFTVAQKTHLV 
                   
               
               
                   
                   
                   
                   
                 SGTLGYEAQLVNAVISSSRAIVGRFHYRFSGGWERLVGKVGYE 
                   
               
               
                   
                   
                   
                   
                 KQQRTNFKTKAKWEVTVATKKWLPEDEAGLWVECGAVVAG 
                   
               
               
                   
                   
                   
                   
                 ESEITWGPKLYLASILVRNSELWVTKPQQQIAYASLKDWSRLY 
                   
               
               
                   
                   
                   
                   
                 TPAVMQGVYDREELAQPSFENIRDITPEIKPIKQLNNEPSIDSLM 
                   
               
               
                   
                   
                   
                   
                 GGDAVEAETAGSNEPNLNALVSGDIEMMQNDDTPSVFETTRD 
                   
               
               
                   
                   
                   
                   
                 LITDISDIQECVNYRRDIETMKNNQTITANEFSILKKMIKTVHNT 
                   
               
               
                   
                   
                   
                   
                 FNVQG 
                   
               
               
                   
               
               
                 PF079 
                 SSAP 
                 recT 
                   Pirellula  sp SH-Sr6A 
                 MTAAAEERTEHVSGASPKRSEAMILLEDLQSDDTSRKLLQILPS 
                 213 
               
               
                   
                   
                   
                   
                 HMKPEFFVRVVINQLNKNPKLALCTRQSFIGSVLDLAAIGLEPD 
                   
               
               
                   
                   
                   
                   
                 GRRAHLIPYGRTCTYQLDYKGIAELVLRSGKVSHVAADVIRRG 
                   
               
               
                   
                   
                   
                   
                 DIFVWNKGQLKEHVPHFLRTDAKAPKEAGEVFAAYSLVVFKD 
                   
               
               
                   
                   
                   
                   
                 GTERAEVMSRADILAIRDGSPGWQAFVAKKSNDTPWDPRFPH 
                   
               
               
                   
                   
                   
                   
                 KEFEMWKKTTFKRLSKWLPISAEAQAAIEKEDRNDYGDRNSN 
                   
               
               
                   
                   
                   
                   
                 VINSPSVRTVRSADDLKEELKRRNAESTSTEVIHDTEFVAPEGIS 
                   
               
               
                   
                   
                   
                   
                 KQAELLELKAATDPLTIIEIERDLEHVRAEDRDEVLAAIASAKA 
                   
               
               
                   
                   
                   
                   
                 RL 
                   
               
               
                   
               
               
                 PF080 
                 SSAP 
                 recT 
                   Ahrensia  sp R2A130 
                 MNAIARLESGIDRSISNEIAVGSSGLTFQNAGQIMEYAKMMAVS 
                 214 
               
               
                   
                   
                   
                   
                 GSAVPKHLRGNAGACLGIVDDALRFQMSPYALARKSYFVNDN 
                   
               
               
                   
                   
                   
                   
                 LGYEAQVLAAIVISRSPLRERPNVNFEGEGQDRVCIVSATFNDG 
                   
               
               
                   
                   
                   
                   
                 AEREVRSPAFKAITPKNSPLWKSDPDQQHSYYTLRAFARRHCP 
                   
               
               
                   
                   
                   
                   
                 DVLLGMYDPEELSSMAARDVTPPRAPTAQERLKSIAAEKPAEP 
                   
               
               
                   
                   
                   
                   
                 VLHNEGFGTGNMLEDAPASAVSEPEQSQADTGEIVDQQEQEPA 
                   
               
               
                   
                   
                   
                   
                 PAVETRIEPERTTLNQDDKDVLTDFIIPFWKAESEEARKMVRM 
                   
               
               
                   
                   
                   
                   
                 KHDPMIEARGKLATTAARKMVEAVIAGKPAEAGEVIGLTMQD 
                   
               
               
                   
                   
                   
                   
                 MEELS 
                   
               
               
                   
               
               
                 PF081 
                 SSAP 
                 recT 
                   Borrelia duttonii  CR2A 
                 MTKNNILKNQSTNTVDVIIEKMNSSNIAEVWETYKIMHNLKKI 
                 215 
               
               
                   
                   
                   
                   
                 DAYSEREILTLLQVNKLNPFKKEAYIIPFNGRYTVVVAYQTLLI 
                   
               
               
                   
                   
                   
                   
                 RAYEAGYNKYDLDFEEKLVKSLKIDSKGNKMIQEDWQCTAFF 
                   
               
               
                   
                   
                   
                   
                 KSDDGIRYSFSVLLSEYFKNTPIWREKPVFMLRKCAVSCLCRTL 
                   
               
               
                   
                   
                   
                   
                 PGSGLESMPYIREELEDMGTVLQQELQGFEEVNNSTPEATIEIQS 
                   
               
               
                   
                   
                   
                   
                 VNHNSGDDINKSIPTKYYCYQNLLIAARNMYNFVSDKPFGSLS 
                   
               
               
                   
                   
                   
                   
                 EINTYLESVKSGDDSKLLEYFNTNKMLKSIEYWCNLLKEYFTK 
                   
               
               
                   
                   
                   
                   
                 SSRDLSRLEKFNIFMSFDLDKVGNSPLKLFSQLSITKEFQCLFSL 
                   
               
               
                   
                   
                   
                   
                 T 
                   
               
               
                   
               
               
                 PF082 
                 SSAP 
                 recT 
                   Candidatus Accumulibacter  sp 
                 MSKTLRNLQKSRQGTPANAVSFPVMLEQFKGEIARALPRHLSP 
                 216 
               
               
                   
                   
                   
                 SK-12 
                 ERIVRVALTAFRLNPKLADVDPRSIFAAVIQSSQLGLEVGLMGE 
                   
               
               
                   
                   
                   
                   
                 AHLVPFGSQCQLIPAYQGLMKLARNSGLVQDIYGHEVRINDRF 
                   
               
               
                   
                   
                   
                   
                 DIVLGLHRSLMHEPLKQNGFPAADDERGAIVGFYVVAVFKDGT 
                   
               
               
                   
                   
                   
                   
                 RTFYALSREQVEQVRDHSRGYQMARKLRKESPWDTHFVSMGL 
                   
               
               
                   
                   
                   
                   
                 KTVIRRVCNLLPKSPELAMALAMDELNERGETQNLGVTEAIDG 
                   
               
               
                   
                   
                   
                   
                 SWAPVLDEQPDDPGQASDPARASTRKPLADLLAGIGQALSLEA 
                   
               
               
                   
                   
                   
                   
                 LDEVYAQAEDAVAGDDLERLLRAYRSRKAALTPPLSPSSPIRG 
                   
               
               
                   
                   
                   
                   
                 VVNNASA 
                   
               
               
                   
               
               
                 PF083 
                 SSAP 
                 recT 
                   Bifidobacterium reuteri  DSM 
                 MGQLARSIQNRQLQAMPNDQERKQQFMTALEKDWPRIVASMP 
                 217 
               
               
                   
                   
                   
                 23975 
                 KHMTPDRIFQMYQSTLSREPKLRECTLNSVLSCFMKCAQLGLE 
                   
               
               
                   
                   
                   
                   
                 LSNADGLGKAYIIPFFNNKNNEMEATFLIGYRGMLQLARNSGEI 
                   
               
               
                   
                   
                   
                   
                 KSMQAKAVYDGDEFHYQFGLHEDLVHIPAEKRPRNAKLTHVY 
                   
               
               
                   
                   
                   
                   
                 FIALFKDGGHQLDVMTRDEVDAVRSRSKSKNNGPWVTDYEA 
                   
               
               
                   
                   
                   
                   
                 MAIKSVIRRAFKMLPNSADVKEEVQQHVDAYTPDYSGILPSSPE 
                   
               
               
                   
                   
                   
                   
                 GLPDSDADAPEEPSDIDGVLADGGTEQPAEEPDPVEVKRRDVIR 
                   
               
               
                   
                   
                   
                   
                 RFQQLGVTSDAEACGTISKVLGREVKETGGLPEADEDVVLAQL 
                   
               
               
                   
                   
                   
                   
                 KASVREGE 
                   
               
               
                   
               
               
                 PF084 
                 SSAP 
                 recT 
                 
                   Serratia odorifera DSM 4582 
                 
                 MSTEIIEQKKNGIIDNVSILTNGDLFDRLMKISEVMAKSGAMVP 
                 218 
               
               
                   
                   
                   
                   
                 QHFRDQPDACMAITMQAARWGMDPFVVAQKTHLVNGTLGYE 
                   
               
               
                   
                   
                   
                   
                 AQLVNAIINSMAPTKDRIHFEWFGPWENILGCFVEKTGKSGNK 
                   
               
               
                   
                   
                   
                   
                 YIAPGWSLADEKGVGVRAWATLKGEDEPRELVLYLSQAQVRN 
                   
               
               
                   
                   
                   
                   
                 STLWASDPRQQLAYLSVKRWARLYCPDVILGIYSDDELLEPTP 
                   
               
               
                   
                   
                   
                   
                 RAEKDITPVASAVTELDSPPTSTEPDAGKAAELAEALSAALDEA 
                   
               
               
                   
                   
                   
                   
                 STQEEAATIEQRIAKHKDALGSSLLFSLRGKAQKKRSGFRGVSE 
                   
               
               
                   
                   
                   
                   
                 IDAAFSALDGTDNQKFQQLETLVANWKNALPPADVERFTLAL 
                   
               
               
                   
                   
                   
                   
                 DDLRPEYQQ 
                   
               
               
                   
               
               
                 PF085 
                 SSAP 
                 recT 
                   Persephonella marina  (strain DSM 
                 METKTLAPPTALMGNVDYDKLMQQAGQLILRDREGKPYTNEQ 
                 219 
               
               
                   
                   
                   
                 14350/EX-H1) 
                 RALIVLAAQQLGLNPILGHLTIIQDRLYITNAGLLHIAHNSGKLQ 
                   
               
               
                   
                   
                   
                   
                 GIKTRPATEEERKAYFLGNNPKDIHLWRAEVYLKGQKEPYIGW 
                   
               
               
                   
                   
                   
                   
                 GKASTNEKSWAVKSNPQEMAETRAVNRALRKAFNVAGYTSV 
                   
               
               
                   
                   
                   
                   
                 EEIDEEPQFINEEDDEEPITQEQVKILHAIANLISKDFYDHEFKN 
                   
               
               
                   
                   
                   
                   
                 WLREKTGKESSKELTKSEASDIADRLLTVLEDKLKFLADEAGIN 
                   
               
               
                   
                   
                   
                   
                 YYKLLNDKYSIGTLSETDNYYIWKEVRDYIEEAYKRKGLLKSIL 
                   
               
               
                   
                   
                   
                   
                 EVAQEKNISNEEIKQIIFKEFGKESSKQLTIDELERLLNIVENI 
                   
               
               
                   
                   
                   
                   
                 SFEPSDEDVPF 
                   
               
               
                   
               
               
                 PF086 
                 SSAP 
                 recT 
                 Parcubacteria group bacterium 
                 MSNFQNAVAIIAKQESKFMELVKAASTDVVFKQEMLYASQAM 
                 220 
               
               
                   
                   
                   
                 GW2011_GWA2_42_14 
                 MNNDYLCKTAINNPLSLRNAFESQVAACGLTLNPSRGLCYLVPR 
                   
               
               
                   
                   
                   
                   
                 DGQVILDVSYKGMIKTAVNDGAIRDCIVELVYSNDKFVYKGKR 
                   
               
               
                   
                   
                   
                   
                 HSPVHEFDPFDLKEQRGEFRGVYVEVTLPDGRVHVEAVTAVDI 
                   
               
               
                   
                   
                   
                   
                 YKARNASDLWTRKKKGPWVDFEDSMRKKSGIKIAKKYWPQV 
                   
               
               
                   
                   
                   
                   
                 GEKLDSVIHYLNTDAGEGFASQDVPVSVVERYMGQVEQVEPA 
                   
               
               
                   
                   
                   
                   
                 EPLPTSNETVPVVASVAVAEQAAATQSQPTPESEAKAPLQGTV 
                   
               
               
                   
                   
                   
                   
                 DPNVEAAESALPERTLNKVEELVKRARNSGAWKAAHEYISTW 
                   
               
               
                   
                   
                   
                   
                 PEIARDYATKKLKAAEYQLAASGE 
                   
               
               
                   
               
               
                 PF087 
                 SSAP 
                 recT 
                 
                   Xanthobacter autotrophicus 
                 
                 MNALAPINVQSSAIRAMVPQTMDEVFHLADAVHRSGLAPTGL 
                 221 
               
               
                   
                   
                   
                 (strain ATCC BAA-1158/Py2) 
                 KNVQAVAIAIMTGLELGVPPMTALQRIAVINGRPTIWGDLAIAL 
                   
               
               
                   
                   
                   
                   
                 VRASGLAETIKEEILGEGDDRVAICTVKRKGDPQQVVGSFSVA 
                   
               
               
                   
                   
                   
                   
                 DAKRARLWDPREKVQRRRQDGNGTYEALNDAPWHRFPERM 
                   
               
               
                   
                   
                   
                   
                 MKMRARAFALRDGFADVLGGLYLREELEEEQVDIRDVTPPTAP 
                   
               
               
                   
                   
                   
                   
                 PPTITEEKKAPAPPAPAPAALAPPDPHVDLPSPLDRVTTPEVRD 
                   
               
               
                   
                   
                   
                   
                 MVLNSPSAPAGLAQKAPAPPPPSVASVPAREERAPSPGEGAPRP 
                   
               
               
                   
                   
                   
                   
                 DRAPSPAPFPNMAPHLVSLWAELGKCQNPTALENTWAFEEDDI 
                   
               
               
                   
                   
                   
                   
                 NTWSMADRESAAALYERRLAELCRKG 
                   
               
               
                   
               
               
                 PF088 
                 SSAP 
                 recT 
                   Ruminococcus  sp SR1/5 
                 MANEMTVQKTESLSNSEAFTNKVLKEFGSNVAGNIQVTDYQR 
                 222 
               
               
                   
                   
                   
                   
                 QLIQGYFIATDRALKMAEEKRVSKNENNKDHKWDNLDPINWN 
                   
               
               
                   
                   
                   
                   
                 TVDLNALALDVVHYARMGLDMMQDNHLSAIPFKDNNRLSRT 
                   
               
               
                   
                   
                   
                   
                 GTKMYVVNLMPGYNGIQYIAEKYALEKPVSVTVELVYSTDTF 
                   
               
               
                   
                   
                   
                   
                 KPLKKNRENRVESYDFEINNAFDRGEIVGGFGYIEYTEPTKNKL 
                   
               
               
                   
                   
                   
                   
                 IIMTIKDILKRKPDKASGEFWGGKKTAWEKGQKVEVETEGWFE 
                   
               
               
                   
                   
                   
                   
                 EMCLKTVKREVYSAKNMPRDPKKIDDAYEYMRMQEIRLAQM 
                   
               
               
                   
                   
                   
                   
                 ETQEVIDAEANQVVIDTEAQETPQKPAQPAFLTDDGGQQALDL 
                   
               
               
                   
                   
                   
                   
                 GSPAKPQAQPQPARNTTAARSTATRAAGPTF 
                   
               
               
                   
               
               
                 PF089 
                 SSAP 
                 recT 
                   Sulfurovum  sp ES06-10 
                 MSKNEVTHVSQGQGAMIKSPFSAYALTEEQSNIIKTQIAPGITD 
                 223 
               
               
                   
                   
                   
                   
                 GDLMYCLEIAKQAQLNPIIKEIYFVPRRSKIDNQWVTKHEPMIG 
                   
               
               
                   
                   
                   
                   
                 RKGARSIARRKGMIVPPTTGYTLKQFPFLENGEWKEKRDLIGW 
                   
               
               
                   
                   
                   
                   
                 AELEITGQKVRKEAAYSVFVQKTSEGAVTKFWSTMPTVMIEKV 
                   
               
               
                   
                   
                   
                   
                 AEFQLLDAVYGLDGLMYMDAGYIEDESSSTQEMSSLADLGKIE 
                   
               
               
                   
                   
                   
                   
                 RELKSLNLEYHLVGDEIRIQDKGAFQFAAALKKEGFVYENSTK 
                   
               
               
                   
                   
                   
                   
                 FWKIRVAMVENADIEAPKELPKPEPEQIDPKKELANAKKALMK 
                   
               
               
                   
                   
                   
                   
                 VLLGNGLTKEEAGEFAQTLDVSNVDSINKLLPGNGGHDELIAKI 
                   
               
               
                   
                   
                   
                   
                 NVFLTPQSSQTHQDNLFDGDEEENPFG 
                   
               
               
                   
               
               
                 PF090 
                 SSAP 
                 recT 
                 Microgenomates group bacterium 
                 MEVKNELAIQFDKTKAVMRSEQAIERFAEALGSGQQAKRFIGS 
                 224 
               
               
                   
                   
                   
                 GW2011_GWF1_44_10 
                 VLLVVSQSDRLMECTPQSIMVSGVRAATLKLSVDPSLGHAVIV 
                   
               
               
                   
                   
                   
                   
                 PYKNKGIPTATFIIGYKGLKQLAYRTGQYAFINEKIVYDGQTIEE 
                   
               
               
                   
                   
                   
                   
                 DDFSGIQRVRGIPTTYGKDGWKPIGYWMGFELKNGFRQTFYM 
                   
               
               
                   
                   
                   
                   
                 TIEEVEAHAKRYSKTYDKVKKQFYPDSLWHTDFDVMAIKTVIR 
                   
               
               
                   
                   
                   
                   
                 LGLSKYGYFDEDSLLAMAESSEFDDDLVVDGELIEDALEQEAE 
                   
               
               
                   
                   
                   
                   
                 REQAREAEHAGKSTEQLSSLLGFEDPPDTSKKEPVKKAAAKEA 
                   
               
               
                   
                   
                   
                   
                 VTKVKPKEYEVAASFNSKRLNKLYGEMSVEELQGEIMALTQY 
                   
               
               
                   
                   
                   
                   
                 ETKTQEEKVQVNDRIQAAKELIRYIESNVL 
                   
               
               
                   
               
               
                 PF091 
                 SSAP 
                 recT 
                   Mycobacterium marinum  (strain 
                 MTEIATIDETTTDLIRGQVGFNSTQRAMLAQLGLSDAPEGDLIL 
                 225 
               
               
                   
                   
                   
                 ATCC BAA-535/M) 
                 FSHVCQKSGLDPFRREIFMIGRNTQVTRYEKVDPDDPESNQRK 
                   
               
               
                   
                   
                   
                   
                 VTRWETVYTIQTGIQGFRKRARELADEKGDRLGFDGPYWCGE 
                   
               
               
                   
                   
                   
                   
                 DGNWKEIWPDTDKPVAAKYIVFRNGEPVPAVTHYSEYVQTTK 
                   
               
               
                   
                   
                   
                   
                 VDGVAQPNSMWSKMPRNQLAKCAEALALQRAYPDELSGIVLE 
                   
               
               
                   
                   
                   
                   
                 DAAQVIDSDGQIINETQRPPARARGAAALRDRAKAEAKPDDPE 
                   
               
               
                   
                   
                   
                   
                 AVNADVVEPRHEDVASQPRPMSDGSRRKWLNRMFQLLGESDC 
                   
               
               
                   
                   
                   
                   
                 TEQESQLTVIAKLANAPGIEHRDQLDDGQLKGVVNQLNQWEK 
                   
               
               
                   
                   
                   
                   
                 SGQLVDKVADILDQAAIDEAQAAEQDAQQTIDGGN 
                   
               
               
                   
               
               
                 PF092 
                 SSAP 
                 recT 
                 
                   Mameliella alba 
                 
                 MNTQIAKIPLRQVQDVKTLLHNDQARQQLAAVAAKHMSPERL 
                 226 
               
               
                   
                   
                   
                   
                 MRVTANAIRTTPKLQEADPLSFLGALMQCAALGLEANTVLGH 
                   
               
               
                   
                   
                   
                   
                 AYLVPFKNNRKGITEVQVIIGYKGFIDLARRSGHITSISAGIHYS 
                   
               
               
                   
                   
                   
                   
                 DDELWEHEEGTEARLRHRPGPQEGEKLHAYAIAKFTDGGHAY 
                   
               
               
                   
                   
                   
                   
                 VVLPWARVMKIRDGSQNWQSAVKFGKTKQSPWYTHEDAMAS 
                   
               
               
                   
                   
                   
                   
                 KTAIRALAKYLPLSVEMADAITIDHDEGTRVDYAAFAQSPEDG 
                   
               
               
                   
                   
                   
                   
                 PQVEEGEEIDGEATEVDEGDRHQQDADPKGATGETEEKPNQKT 
                   
               
               
                   
                   
                   
                   
                 KERKPEAKKEQDKPKDDDAGIPPASDPKFVKLFQDIEAELTDG 
                   
               
               
                   
                   
                   
                   
                 APARGILRFHEAALAEVEKEDPDLHGQIMSMIREAEAND 
                   
               
               
                   
               
               
                 PF093 
                 SSAP 
                 recT 
                   Gordonia soli  NBRC 108243 
                 MSSTEIATTTGAVATQPTSDLAIQPNQTEFTSVQRAALAQLGIE 
                 227 
               
               
                   
                   
                   
                   
                 EATDEDVQVFFHQAKRTGLDPFARQIYMIGRRTKVKEWDPNQ 
                   
               
               
                   
                   
                   
                   
                 RKQIEKWVMKQTIQIGIDGYRLGGRRIASALGIKLEKDGPHWH 
                   
               
               
                   
                   
                   
                   
                 DGNGWVDVWLDPARPPAAARYSITRDGETFTATAMYSEYVQT 
                   
               
               
                   
                   
                   
                   
                 YNTQQGPQPNSMWSKMPANQLAKCAEAAAWRQAFPDQFSGV 
                   
               
               
                   
                   
                   
                   
                 IFEDAAQHTVIDADVIEEEPKTKQGSRGTAGLAAALGVDESTA 
                   
               
               
                   
                   
                   
                   
                 DEPEPQDGPVGTIESGLISAEPSETPEDEADSSHEPRPEPKKPTQ 
                   
               
               
                   
                   
                   
                   
                 KQIHALNALLSQAGLTKAEKKGRQIVVSSFLPNREDPAAALTA 
                   
               
               
                   
                   
                   
                   
                 DETEHVTTQLSALVENQGEQALIDTVEALIQQHDQQGAE 
                   
               
               
                   
               
               
                 PF094 
                 SSAP 
                 recT 
                   Sphingopyxis  sp (strain 113P3) 
                 MNAQTQIATRADNPLAILKTQIDERAKEFQAALPSHISPEKFQR 
                 228 
               
               
                   
                   
                   
                   
                 TILTAVQADPELLKASRKSLILACMKAANDGLLPDRREAALIVE 
                   
               
               
                   
                   
                   
                   
                 KRNYKDAQGAWQQALEVQYLPMVFGLRKKILQSKEVTDIKPN 
                   
               
               
                   
                   
                   
                   
                 VVYRREVEEGHFIYEEGTEAMLRHKPILDLTDEESSDDNIVVAY 
                   
               
               
                   
                   
                   
                   
                 SIATYKDGTKSYEVMRRFEINKVQNCSQTGALIDKRGKPRTPSG 
                   
               
               
                   
                   
                   
                   
                 PWVDWYPEQAKKTVMRRHSKTLPQSGDLVDVEGSEIDQQRA 
                   
               
               
                   
                   
                   
                   
                 ALSAMGALGAGEPIDATPVAPALPQADDLPDHDADTGEIIDTV 
                   
               
               
                   
                   
                   
                   
                 TPDNPNAAEGRADEQHGDQHDGTEEPQGDEDQPYAATVSELIE 
                   
               
               
                   
                   
                   
                   
                 RAAAAGTVIDLNAVEKDWQKHMAALPDVENARIDTAVKERR 
                   
               
               
                   
                   
                   
                   
                 DQLTAK 
                   
               
               
                   
               
               
                 PF095 
                 SSAP 
                 recT 
                   Actinobacteria bacterium  OK074 
                 MTVDTPVTWLAIREDQKAFEDQQLRALRAAFPDLVDASPAQL 
                 229 
               
               
                   
                   
                   
                   
                 GIFFHYCKASGLDPFGRQIYMIKRKSRGEVRWTIQTGIDGYRLI 
                   
               
               
                   
                   
                   
                   
                 ARRAADRAGQSIAYEDEFVWYDAEGGEHAVWLRDEPPVACRV 
                   
               
               
                   
                   
                   
                   
                 VIWRGEARFPAVAHWREYAPKVWDYEAQEYKLGGLWPQMP 
                   
               
               
                   
                   
                   
                   
                 ASQFGKVAEALSLRRACPADLSGLHVDEEMHAADAAESRERV 
                   
               
               
                   
                   
                   
                   
                 KEAAARLRSPDEQQANGGSPAQPTSDVVDAEIVESQTANTDAA 
                   
               
               
                   
                   
                   
                   
                 APQEEQRADKAAEPPASGRTDEVDRVRERMSALDQERSRLEE 
                   
               
               
                   
                   
                   
                   
                 ARARIRETADRLALGFDTVNDRCFDAFGTSFQDASAEQLSELN 
                   
               
               
                   
                   
                   
                   
                 DSLTPAAGTSDEPATAGRRNGSARRTRTPAKKAAASAKKKTA 
                   
               
               
                   
                   
                   
                   
                 RKATDGTTSPTRVSK 
                   
               
               
                   
               
               
                 PF096 
                 SSAP 
                 recT 
                   Rhizobium  sp CF080 
                 MNSLTTVEAGRLIDGLRVELEPLVLDSGKSFDRLRSVFMIAVQ 
                 230 
               
               
                   
                   
                   
                   
                 QNPDILKCSENSIKREISKCAADGLVPDNKEAAMIPYKGELQYQ 
                   
               
               
                   
                   
                   
                   
                 PMVQGIIKRMKELGGVFNIVCNLVFEKDVFTLDESDPDSLSHVS 
                   
               
               
                   
                   
                   
                   
                 DRFATDRGKVVGGYVVFRDEHKRVMHLETMSIVDFENVRKAS 
                   
               
               
                   
                   
                   
                   
                 KAPDSPAWKNWTNEMYKKAVLRRGAKYISVNNDKIRALLER 
                   
               
               
                   
                   
                   
                   
                 QDELFDFTPNRVVERTNPFTGEVIEGNATPSIENKQQPPMQQPR 
                   
               
               
                   
                   
                   
                   
                 ETQPAGSQQEPRQQRINNTGDGRASRKQERQPENKDAGNTQQ 
                   
               
               
                   
                   
                   
                   
                 TKTETKKDLVPPAVPEVDVFPADKAKAAEAAEKLLGVALLPD 
                   
               
               
                   
                   
                   
                   
                 LDPRGRRGVLKQAAVDWKEATPAYTHPLLKACIDMSDWAIQQ 
                   
               
               
                   
                   
                   
                   
                 QVKEEAWAGEHAMFVHKIKSLLDVEKLNVGKYP 
                   
               
               
                   
               
               
                 PF097 
                 SSAP 
                 recT 
                   Bradyrhizobium  sp STM 3843 
                 MNSARGWWLDKNLVKLARRTAFKETNEDEFDQAVAFCREKN 
                 231 
               
               
                   
                   
                   
                   
                 LSPMSGQLYAFVFNKDDAKKRNMVIVTSIMGYRAIANRSGDY 
                   
               
               
                   
                   
                   
                   
                 MPGPTKAFFDPGAKNSLINPRGLVRAEGGADRFIHGGWKNVTE 
                   
               
               
                   
                   
                   
                   
                 EALWESFAPIIKSGSDDDAYEWVDTGEVWADSGKPKKKRRLR 
                   
               
               
                   
                   
                   
                   
                 QGAEVQEILDPKKEGWHRMPDVMLKKCAEAAALRRGWPEDL 
                   
               
               
                   
                   
                   
                   
                 SGLYVEEEVHRSQVIDADYVDLTPSEMVAKAETDARIEKIGGPS 
                   
               
               
                   
                   
                   
                   
                 IFAAFDAAGTLERVEIGKFFDRVDAHTRNLKPEDVASFAVRNR 
                   
               
               
                   
                   
                   
                   
                 EALREFWGRAKNDALTLKTILETRSSAATPASQANGHDGAAGT 
                   
               
               
                   
                   
                   
                   
                 AAPRSESQTDSGPDTGAADPSLSSDAAAKLKSALLADVAQLRT 
                   
               
               
                   
                   
                   
                   
                 RSDFDSWERDAKAHLEKLPEQMRSEVQAELDHRRADIR 
                   
               
               
                   
               
               
                 PF098 
                 SSAP 
                 recT 
                 
                   Nitratireductor basaltis 
                 
                 MNQIATSTQRELAEKDKFRQQMKGQSEAFCEALIGSNIPPEKFQ 
                 232 
               
               
                   
                   
                   
                   
                 RVVATAVMTDTNILFADRKSLMEATMRAAQDGLLPDKREGAF 
                   
               
               
                   
                   
                   
                   
                 VLFKNRVQWMPMIGGIIKKIHQSGDISLITAKVVYGGDTYRTW 
                   
               
               
                   
                   
                   
                   
                 VDDEGEHVLYEPAEEPDHNVIRQVFAMAKTKDGTLYVEALTT 
                   
               
               
                   
                   
                   
                   
                 RDIEKIRSVSRSGEKGPWKDWWEEMAKKSAIRRLAKRLPLSSD 
                   
               
               
                   
                   
                   
                   
                 IHDLIQRDNEMYDESRAPEPRQSVMARFRASATPQIEMDRGEG 
                   
               
               
                   
                   
                   
                   
                 FDIDHITRETGTLTSEDAEQVSSASPSLADEGDGADPAPETPSTV 
                   
               
               
                   
                   
                   
                   
                 ATESEPAEAADQAEEASTEQASTPEASHGDEAGASSAVNPQTY 
                   
               
               
                   
                   
                   
                   
                 AAMTECIDRLLGAATDTSGGDVEKRKEKVEKFAAAWCNELPD 
                   
               
               
                   
                   
                   
                   
                 HTAFVDKCKDTALRIAEKPAERAKAEEYLKAKLPEVEA 
                   
               
               
                   
               
               
                 PF099 
                 SSAP 
                 recT 
                 
                   Lentibacillus amyloliquefaciens 
                 
                 MEKAIQFSNSEKSLIWKRFIEPAKGTQEEAEHFLEVCENFGLNP 
                 233 
               
               
                   
                   
                   
                   
                 LLGDIVFQRYETKRGAKTQFITTRDGLELRVATSQPGYVGPPNA 
                   
               
               
                   
                   
                   
                   
                 NVVKEGDHFEFLPSEGTVRHKFGTKRGQILGAYAIMQHKKHNP 
                   
               
               
                   
                   
                   
                   
                 VAVFVDFEEYELANSGRQNSRYGNPNVWDTLPSAMIIKIAETF 
                   
               
               
                   
                   
                   
                   
                 VLRRQFPLGGLYTQEEMGLDDNLQTEDAKETASPDKQHSAQT 
                   
               
               
                   
                   
                   
                   
                 KPAAKEPEKEVSQPGDDVIHQEMVVKSYDIKTSSSKKQVGVLS 
                   
               
               
                   
                   
                   
                   
                 VQSKTSNQAVQVLIRDKSLMKSLQHVSAGEILNLELYEENSFVF 
                   
               
               
                   
                   
                   
                   
                 LKDVAERASVDNQQKDEKEENQKGESSKETDGPAKAPQENEQ 
                   
               
               
                   
                   
                   
                   
                 SEAGYPVEVMIQNVKFGEKASEKFAKITGVIDGQTQLMLARGE 
                   
               
               
                   
                   
                   
                   
                 QAVQKADGLEQGDNVTLSLKKENGFLFLVDLVEETQQRAG 
                   
               
               
                   
               
               
                 PF100 
                 SSAP 
                 recT 
                 
                   Oligotropha carboxidovorans 
                 
                 MAKTETRERTQADHRSAEPAPNVNVPAQKSNHPVAVFREYAM 
                 234 
               
               
                   
                   
                   
                 (strain ATCC 49405/DSM 1227/ 
                 QRISTLQELPHIDPQQLLSVALTAIQRKPDLMRCTPQSLWNACV 
                   
               
               
                   
                   
                   
                 KCTC 32145/OM5) 
                 LAAQDGLLPDGREGAIVPYGENADGKRVAEIATWMPMVEGLR 
                   
               
               
                   
                   
                   
                   
                 KKVRNSGQIKDWYVELVYAGDFFRYRKGDDPRLEHEPVPPSQ 
                   
               
               
                   
                   
                   
                   
                 RTPNTPFHGIVAAYSIAVFTDGSKSAPEVMWIEEIEKVRTKSKA 
                   
               
               
                   
                   
                   
                   
                 KNGPWQDSAFYPEMCKKVVARRHYKQLPHSAGMDKLIQRDD 
                   
               
               
                   
                   
                   
                   
                 DDYDFDRQDEALVQQRQHRRLVSTTSAFDEFARNGQTIDHRAS 
                   
               
               
                   
                   
                   
                   
                 DPVHQDGDDEFAEDEPSHDETGADETDRTSANSKPETGGAAD 
                   
               
               
                   
                   
                   
                   
                 QREEAAVNSKDEQQQHAEPQQQTQAEQTTANDGKPAAKFDPH 
                   
               
               
                   
                   
                   
                   
                 VGEEVRRWPPGAVPSDPDEYEFYVETKLSDYTRETADKIPDW 
                   
               
               
                   
                   
                   
                   
                 WKSAEEKKLREACGISKQRHDELRNKAASRKTELLKG 
                   
               
               
                   
               
               
                 SSB_01 
                 SSB 
                 Viral- 
                   Streptococcus pyogenes  serotype 
                 MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNG 
                 235 
               
               
                   
                   
                 SSB 
                 M28 (strain 
                 EREADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQ 
                   
               
               
                   
                   
                   
                 MGAS6180),  Streptococcus   
                 QGQRVYVTEVVADNFQMLESRATREGGSTGSFNGGFNNNTSS 
                   
               
               
                   
                   
                   
                   pyogenes ,  Temperate  phage 
                 SNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDDLPF 
                   
               
               
                   
                   
                   
                 phiNIH11,  Streptococcus pyogenes   
                   
                   
               
               
                   
                   
                   
                 serotype M2 (strain 
                   
                   
               
               
                   
                   
                   
                 MGAS10270),  Streptococcus   
                   
                   
               
               
                   
                   
                   
                   pyogenes  serotype M3 (strain 
                   
                   
               
               
                   
                   
                   
                 ATCC BAA-595/ 
                   
                   
               
               
                   
                   
                   
                 MGAS315),  Streptococcus   
                   
                   
               
               
                   
                   
                   
                   pyogenes  STAB902 
                   
                   
               
               
                   
               
               
                 SSB_02 
                 SSB 
                 Bacterial- 
                 
                   Streptococcus pyogenes 
                 
                 MINNVVLVGRMTKDAELRYTASQVAVATFTLAVNRRFKEQNG 
                 236 
               
               
                   
                   
                 SSB 
                 STAB902,  Streptococcus   
                 EREADFINCVIWRQSAENLANWAKKGALIGVTGRIQTRNYENQ 
                   
               
               
                   
                   
                   
                   pyogenes ,  Streptococcus pyogenes   
                 QGQRVYVTEVVADNFQMLESRNQQSGQGNSSQNDNSQPFGNS 
                   
               
               
                   
                   
                   
                 serotype M3 (strain ATCC BAA- 
                 NPMDISDDDLPF 
                   
               
               
                   
                   
                   
                 595/MGAS315) 
                   
                   
               
               
                   
               
               
                 SSB_03 
                 SSB 
                 Bacterial- 
                 
                   Bacillus subtilis 
                 
                 MLNRVVLVGRLTKDPELRYTPNGAAVATFTLAVNRTFTNQSG 
                 237 
               
               
                   
                   
                 SSB 
                   
                 EREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTRNYEN 
                   
               
               
                   
                   
                   
                   
                 QQGQRVFVTEVQAESVQFLEPKNGGGSGSGGYNEGNSGGGQY 
                   
               
               
                   
                   
                   
                   
                 FGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDGKPIDISDD 
                   
               
               
                   
                   
                   
                   
                 DLPF 
                   
               
               
                   
               
               
                 SSB_04 
                 SSB 
                 Bacterial- 
                   Saccharomyces cerevisiae  (strain 
                 MSSVQLSRGDFHSIFTNKQRYDNPTGGVYQVYNTRKSDGANS 
                 238 
               
               
                   
                   
                 SSB 
                 ATCC 204508/S288c) (Baker\′s 
                 NRKNLIMISDGIYHMKALLRNQAASKFQSMELQRGDIIRVIIAEP 
                   
               
               
                   
                   
                   
                 yeast),  Saccharomyces cerevisiae   
                 AIVRERKKYVLLVDDFELVQSRADMVNQTSTFLDNYFSEHPNE 
                   
               
               
                   
                   
                   
                 YJM1250,  Saccharomyces   
                 TLKDEDITDSGNVANQTNASNAGVPDMLHSNSNLNANERKFA 
                   
               
               
                   
                   
                   
                   cerevisiae  YJM451 
                 NENPNSQKTRPIFAIEQLSPYQNVWTIKARVSYKGEIKTWHNQR 
                   
               
               
                   
                   
                   
                   
                 GDGKLFNVNFLDTSGEIRATAFNDFATKFNEILQEGKVYVVSK 
                   
               
               
                   
                   
                   
                   
                 AKLQPAKPQFTNLTHPYELNLDRDTVIEECFDESNVPKTHFNFI 
                   
               
               
                   
                   
                   
                   
                 KLDAIQNQEVNSNVDVLGIIQTINPHFELTSRAGKKFDRRDITIV 
                   
               
               
                   
                   
                   
                   
                 DDSGFSISVGLWNQQALDFNLPEGSVAAIKGVRVTDEFGGKSLS 
                   
               
               
                   
                   
                   
                   
                 MGFSSTLIPNPEIPEAYALKGWYDSKGRNANFITLKQEPGMGG 
                   
               
               
                   
                   
                   
                   
                 QSAASLTKFIAQRITIARAQAENLGRSEKGDFFSVKAAISFLKVD 
                   
               
               
                   
                   
                   
                   
                 NFAYPACSNENCNKKVLEQPDGTWRCEKCDTNNARPNWRYIL 
                   
               
               
                   
                   
                   
                   
                 TISIIDETNQLWLTLFDDQAKQLLGVDANTLMSLKEEDPNEFTK 
                   
               
               
                   
                   
                   
                   
                 ITQSIQMNEYDFRIRAREDTYNDQSRIRYTVANLHSLNYRAEAD 
                   
               
               
                   
                   
                   
                   
                 YLADELSKALLA 
                   
               
               
                   
               
               
                 SSB_05 
                 SSB 
                 Bacterial- 
                 
                   Saccharomyces cerevisiae 
                 
                 MATYQPYNEYSSVTGGGFENSESRPGSGESETNTRVNTLTPVTI 
                 239 
               
               
                   
                   
                 SSB 
                   
                 KQILESKQDIQDGPFVSHNQELHHVCFVGVVRNITDHTANIFLTI 
                   
               
               
                   
                   
                   
                   
                 EDGTGQIEVRKWSEDANDLAAGNDDSSGKGYGSQVAQQFEIG 
                   
               
               
                   
                   
                   
                   
                 GYVKVFGALKEFGGKKNIQYAVIKPIDSFNEVLTHHLEVIKCHS 
                   
               
               
                   
                   
                   
                   
                 IASGMMKQPLESASNNNGQSLFVKDDNDTSSGSSPLQRILEFCK 
                   
               
               
                   
                   
                   
                   
                 KQCEGKDANSFAVPIPLISQSLNLDETTVRNCCTTLTDQGFIYPT 
                   
               
               
                   
                   
                   
                   
                 FDDNNFFAL 
                   
               
               
                   
               
               
                 SSB_06 
                 SSB 
                 Bacterial- 
                 
                   Saccharomyces cerevisiae 
                 
                 MASETPRVDPTEISNVNAPVERIIAQIKSQPTESQLILQSPTISS 
                 240 
               
               
                   
                   
                 SSB 
                   
                 KNGSEVEMITENNIRVSMNKTFEIDSWYEFVCRNNDDGELGFLI 
                   
               
               
                   
                   
                   
                   
                 LDAVLCKFKENEDLSLNGVVALQRLCKKYPEIY 
                   
               
               
                   
               
               
                 SSB_07 
                 SSB 
                 Viral- 
                   Staphylococcus  phage phi11 
                 MKITGRTQYIQETNQEAFMKGGDFLGAGEFTVKVANVEFNDR 
                 241 
               
               
                   
                   
                 SSB 
                 (Bacteriophage phi11), 
                 ENRYFTIVFENNEGKQYKHNQFVPPFQQDYQEKQYIELLSREGI 
                   
               
               
                   
                   
                   
                   Staphylococcus  phage 
                 KLNLPDLTFDTDQLINKIGTIVLKNKFNEEQGKVFVRLSYVKV 
                   
               
               
                   
                   
                   
                 80,  Staphylococcus  phage 
                 WNKDDEVVNKPEPKTDEMKQKEQQANGKQTPMSQQSNPFAN 
                   
               
               
                   
                   
                   
                 52A,  Staphylococcus aureus   
                 ANGPIEINDDDLPF 
                   
               
               
                   
                   
                   
                 (strain NCTC 8325) 
                   
                   
               
               
                   
               
               
                 SSB_08 
                 SSB 
                 Viral- 
                   Salmonella typhimurium , 
                 MASRGVNKVILVGNLGQDPEVRYMPSGGAVANLTLATSESWR 
                 242 
               
               
                   
                   
                 SSB 
                   Salmonella  phage 
                 DKQTGEMKEQTEWHRVVMFGKLAEVAGEYLRKGSQVYIEGQ 
                   
               
               
                   
                   
                   
                 ST160,  Salmonella  phage ST64T 
                 LRTRKWTDQSGQERYTTEINVPQIGGVMQMLGGRQGGGAPAG 
                   
               
               
                   
                   
                   
                 (Bacteriophage ST64T) 
                 GQQQGGWGQPQQPQQPQGGNQFSGGAQSRPQQSAPAPSNEPP 
                   
               
               
                   
                   
                   
                 MDFDDDIPF 
                   
                   
               
               
                   
               
               
                 SSB_09 
                 SSB 
                 Bacterial- 
                 
                   Enterococcus faecalis 
                 
                 MINNVVLVGRLTKDPDLRYTASGSAVATFTLAVNRNFTNQNG 
                 243 
               
               
                   
                   
                 SSB 
                 TX0309B,  Enterococcus faecalis   
                 DREADFINCVIWRKPAETMANYARKGTLLGVVGRIQTRNYEN 
                   
               
               
                   
                   
                   
                 TX0309A,  Enterococcus faecalis   
                 QQGQRVYVTEVVCENFQLLESRSASEQRGTGGGSFNNNENGY 
                   
               
               
                   
                   
                   
                 (strain ATCC 700802/V583) 
                 QSQNRSFGNNNASSGFNNNNNSFNPSSSQSQNNNGMPDFDKDS 
                   
               
               
                   
                   
                   
                   
                 DPFGGSGSSIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_10 
                 SSB 
                 Viral- 
                   Acyrthosiphon pisum  secondary 
                 MASRGVNKVILIGHLGQDPEVRYMPNGNAVVNMTLATSENW 
                 244 
               
               
                   
                   
                 SSB 
                 endosymbiont phage 1 
                 KDKNTGENKEKTEWHRIVLFGKLAEIAGEYLRKGSQVYIEGSL 
                   
               
               
                   
                   
                   
                 (Bacteriophage APSE-1) 
                 QTRKWQDQNGLERYTTEIIVNIGGTMQMLGNRNSNLQAMTVNDK 
                   
               
               
                   
                   
                   
                   
                 NSTGIKIKKTDAVEFDKKIETHESNKDLTHSPSEIDEDDEIPF 
                   
               
               
                   
               
               
                 SSB_11 
                 SSB 
                 Viral- 
                   Bacillus  phage SPP1 
                 MNSVNLVGRLAADPELRHTNNGTAVVNFIMAVRRNRKDPTTG 
                 245 
               
               
                   
                   
                 SSB 
                 (Bacteriophage SPP1) 
                 QYEADFIRCQAWRGIAEVIANNFGTGRMIGVSGSWRTGAFEGQ 
                   
               
               
                   
                   
                   
                   
                 DGKRVYTNDCVVENITFVDPNKSDSSSPDNSQGSSNTNTFGGS 
                   
               
               
                   
                   
                   
                   
                 QNGSGGQGGYNNDPFANDGKTIDINESDLPF 
                   
               
               
                   
               
               
                 SSB_12 
                 SSB 
                 Viral- 
                   Lactococcus  phage LL-H 
                 MAGINNVVLVGRLTKDVNLRSTQSGTMVGTFTLAVDRTTKDQ 
                 246 
               
               
                   
                   
                 SSB 
                 ( Lactococcus delbrueckii   
                 NGNRQADFIKCVVWNNKYSKMAENLATYAHKGSLIGVQGRIQ 
                   
               
               
                   
                   
                   
                 bacteriophage LL-H) 
                 TRNYDNKDGQRVDVTEVRVDNFSLLESRNSAQREYTGQQGGY 
                   
               
               
                   
                   
                   
                   
                 SQQQGNQPQGNYQASQAANFGQQGQFTAPAGQSDTIDVSNDD 
                   
               
               
                   
                   
                   
                   
                 LPF 
                   
               
               
                   
               
               
                 SSB_13 
                 SSB 
                 Viral- 
                   Escherichia  phage Rtp 
                 MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK 
                 247 
               
               
                   
                   
                 SSB 
                   
                 QTGQQKEQTEWHKVVLFGKLAEIASEYLRKGSQVYIEGKLKTR 
                   
               
               
                   
                   
                   
                   
                 KWTDDAGVERYTTEIIVSQGGTMQMIGARRDDSQSSNGWGQS 
                   
               
               
                   
                   
                   
                   
                 NQPQNHQQYSGGGKPQSNANNEPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_14 
                 SSB 
                 Viral- 
                   Streptococcus  phage 7201 
                 MINNTVLVGRLTKDPEFKYTGSNIAVASFSLAVNRNFKDANGE 
                 248 
               
               
                   
                   
                 SSB 
                   
                 READFINCVIWRQQAENLANWAKKGALIGITGRIQTRSYENQQ 
                   
               
               
                   
                   
                   
                   
                 GQRVYVTEVVAENFQMLESRAAREGGNANNSYSQQQVPNFA 
                   
               
               
                   
                   
                   
                   
                 RKNTEYSNKQPLDISSDDLPF 
                   
               
               
                   
               
               
                 SSB_15 
                 SSB 
                 Viral- 
                   Bacillus  phage 0305phi8-36 
                 MSNELKQVEQTEEAVVVSETKDYIKVYENGKYRRKAKYQQLN 
                 249 
               
               
                   
                   
                 SSB 
                   
                 SMSHRELTDEEEINIFNLLNGAEGSAVEMKRAVGSKVTIVDFIT 
                   
               
               
                   
                   
                   
                   
                 VPYTKIDEDTGVEENGVLTYLINENGEAIATSSKAVYFTLNRLL 
                   
               
               
                   
                   
                   
                   
                 IQCGKHADGTWKRPIVEIISVKQTNGDGMDLKLVGFDKKK 
                   
               
               
                   
               
               
                 SSB_16 
                 SSB 
                 Viral- 
                   Listeria  phage A118 
                 MMNRVVLVGRLTKDPDLRYTPAGAAVATFTLAVNRMFTNQN 
                 250 
               
               
                   
                   
                 SSB 
                 (Bacteriophage A118) 
                 GEREADFINCVVWRKPAENVANFLKKGSMAGVDGRVQTRNY 
                   
               
               
                   
                   
                   
                   
                 EDNDGKRVFVTEVVAESVQFLEPKNNNVEGATSNNYQNKANY 
                   
               
               
                   
                   
                   
                   
                 SNNNQTSSYRADTSQKSDSFASEGKPIDINEDDLPF 
                   
               
               
                   
               
               
                 SSB_17 
                 SSB 
                 Viral- 
                   Lactococcus  phage 
                 MINNVTLVGRITKEPELRYTPQNKAVATFTLAVNRAFKNANGE 
                 251 
               
               
                   
                   
                 SSB 
                 bIL286,  Lactococcus lactis   
                 READFINCVIWGKSAENLANWTHKGQLIGVTGSIQTRNYENQQ 
                   
               
               
                   
                   
                   
                 subsp  lactis  (strain IL1403) 
                 GQRVYVTEVIANNFQVLEKSNQANGERVSNPAAKPQNNDSFG 
                   
               
               
                   
                   
                   
                 ( Streptococcus lactis ) 
                 SDPMEISDDDLPF 
                   
               
               
                   
               
               
                 SSB_18 
                 SSB 
                 Viral- 
                   Lactococcus  phage 
                 MAIITVTAQANEKNTRTVSTAKGDKKIISVPLFEKEKGSSVKVA 
                 252 
               
               
                   
                   
                 SSB 
                 SK1833,  Lactococcus  phage SK1 
                 YGSAFLPDFIQLGDTVTVSGRVQAKESGEYVNYNFVFPTVEKV 
                   
               
               
                   
                   
                   
                   
                 FITNDNSSQSQAKQDLFGGSEPIEVNSEDLPF 
                   
               
               
                   
               
               
                 SSB_19 
                 SSB 
                 Viral- 
                   Mycobacterium  phage Che8/ 
                 MAGDTTITVVGNLTADPELRFTPSGAAVANFTVASTPRMFDRQ 
                 253 
               
               
                   
                   
                 SSB 
                 
                   Mycobacterium smegmatis 
                 
                 SGEWKDGEALFLRCNIWREAAENVAESLTRGSRVIVTGRLKQR 
                   
               
               
                   
                   
                   
                   
                 SFETREGEKRTVVEVEVDEIGPSLRYATAKVNKASRSGGGGGG 
                   
               
               
                   
                   
                   
                   
                 FGSGGGGSRQSEPKDDPWGSAPASGSFSGADDEPPF 
                   
               
               
                   
               
               
                 SSB_20 
                 SSB 
                 Bacterial- 
                   Ureaplasma urealyticum  serovar 
                 MNKVILIGNLVRDPEARQIPSGRLVTNFTIAVNDNTPNANANFI 
                 254 
               
               
                   
                   
                 SSB 
                 10 (strain ATCC 33699/Western),  
                 RCVAWNNQANFLTTYLKKGDAIAIEGRIVSRSYVDNNGKTNY 
                   
               
               
                   
                   
                   
                 
                   Ureaplasma urealyticum 
                 
                 VTEVYADQVQSLSRRNQSPSDNNKVNVDTMMESYTGINTDAA 
                   
               
               
                   
                   
                   
                 serovar 7 str ATCC 
                 FSSNKPQTTLSSTTSNLNKNNDEEDEITSWINLDDDLE 
                   
               
               
                   
                   
                   
                 27819,  Ureaplasma parvum  serovar 
                   
                   
               
               
                   
                   
                   
                 3 (strain ATCC 
                   
                   
               
               
                   
                   
                   
                 700970),  Ureaplasma urealyticum   
                   
                   
               
               
                   
                   
                   
                 serovar 8 str ATCC 
                   
                   
               
               
                   
                   
                   
                 27618,  Ureaplasma urealyticum   
                   
                   
               
               
                   
                   
                   
                 serovar 4 str ATCC 27816 
                   
                   
               
               
                   
               
               
                 SSB_21 
                 SSB 
                 Viral- 
                   Lactococcus lactis  subsp  lactis   
                 MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGE 
                 255 
               
               
                   
                   
                 SSB 
                 bv  diacetylactis  str 
                 READFINCVIWRQQAENLANWAKKGALIGVTGRIQTRNYENQ 
                   
               
               
                   
                   
                   
                 TIFN2,  Lactococcus lactis  subsp 
                 QGQRVYVTEVVADSFQMLESRSAREGMGGGTSAGSYSAPSQS 
                   
               
               
                   
                   
                   
                   lactis  (strain IL1403) 
                 TNNTPRPQTNNNNATPNFGRDADPFGSSPMEISDDDLPF 
                   
               
               
                   
                   
                   
                 ( Streptococcus lactis ), 
                   
                   
               
               
                   
                   
                   
                   Lactococcus  phage bIL309 
                   
                   
               
               
                   
               
               
                 SSB_22 
                 SSB 
                 Bacterial- 
                   Rhizobium loti  (strain 
                 MAGSVNKVILVGNLGADPEIRRLNSGEPVVNIRIATSESWRDK 
                 256 
               
               
                   
                   
                 SSB 
                 MAFF303099) ( Mesorhizobium   
                 NSGERKEKTEWHNVVIFNEGIAKVAEQYLKKGMKVYVEGQLQ 
                   
               
               
                   
                   
                   
                   loti ) 
                 TRKWQDQTGADKYTTEVVLQRFRGELQMLDGRQGEGGQVGG 
                   
               
               
                   
                   
                   
                   
                 YSGGGSSRGSDFGQSGPNESFNRGGGAPRGGGGGGSSRELDDE 
                   
               
               
                   
                   
                   
                   
                 IPF 
                   
               
               
                   
               
               
                 SSB_23 
                 SSB 
                 Bacterial- 
                   Homo sapiens  (Human) 
                 MVDMMDLPRSRINAGMLAQFIDKPVCFVGRLEKIHPTGKMFIL 
                 257 
               
               
                   
                   
                 SSB 
                   
                 SDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQF 
                   
               
               
                   
                   
                   
                   
                 KEDSHPFDLGLYNEAVKIIHDFPQFYPLGIVQHD 
                   
               
               
                   
               
               
                 SSB_24 
                 SSB 
                 Bacterial- 
                   Homo sapiens  (Human) 
                 MVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRL 
                 258 
               
               
                   
                   
                 SSB 
                   
                 LMSDGLNTLSSFMLATQLNPLVEEEQESSNCVCQIHRFIVNTLK 
                   
               
               
                   
                   
                   
                   
                 DGRRVVILMELEVLKSAEAVGVKIGNPVPYNEGLGQPQVAPPAP 
                   
               
               
                   
                   
                   
                   
                 AASPAASSRPQPQNGSSGMGSTVSKAYGASKTFGKAAGPSLSHT 
                   
               
               
                   
                   
                   
                   
                 SGGTQSKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGEGK 
                   
               
               
                   
                   
                   
                   
                 LFSLELVDESGEIRATAFNEQVDKFFPLIEVNKVYYFSKGTLKI 
                   
               
               
                   
                   
                   
                   
                 ANKQFTAVKNDYEMTFNNETSVMPCEDDHHLPTVQFDEFTGID 
                   
               
               
                   
                   
                   
                   
                 DLENKSKDSLVDIIGICKSYEDATKITVRSNNREVAKRNIVLMD 
                   
               
               
                   
                   
                   
                   
                 TSGKVVTATLWGEDADKFDGSRQPVLAIKGARVSDFGGRSLS 
                   
               
               
                   
                   
                   
                   
                 VLSSSTIIANPDIPEAYKLRGWFDAEGQALDGVSISDEKSGGVG 
                   
               
               
                   
                   
                   
                   
                 GSNTNWKTLYEVKSENLGQGDKPDYFSSVATVVVLRKENCM 
                   
               
               
                   
                   
                   
                   
                 YQACPTQDCNKKVIDQQNGLYRCEKCDTEFPNFKYRMILSVNI 
                   
               
               
                   
                   
                   
                   
                 ADFQENQWVTCFQESAEAILGQNAAYLGELKDKNEQAFEEVF 
                   
               
               
                   
                   
                   
                   
                 QNANFRSFIFRVRVKVETYNDESRIKATVMDVKPVDYREYGRR 
                   
               
               
                   
                   
                   
                   
                 LVMSIRRSALM 
                   
               
               
                   
               
               
                 SSB_25 
                 SSB 
                 Bacterial- 
                   Homo sapiens  (Human) 
                 MWNSGFESYGSSSYGGAGGYTQSPGGFGSPAPSQAEKKSRARAQ 
                 259 
               
               
                   
                   
                 SSB 
                   
                 HIVPCTISQLLSATLVDEVFRIGNVEISQVTIVGIIRHAEKAPT 
                   
               
               
                   
                   
                   
                   
                 NIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAG 
                   
               
               
                   
                   
                   
                   
                 HLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKAN 
                   
               
               
                   
                   
                   
                   
                 SQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLI 
                   
               
               
                   
                   
                   
                   
                 KACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTV 
                   
               
               
                   
                   
                   
                   
                 DDDHFKSTDAE 
                   
               
               
                   
               
               
                 SSB_26 
                 SSB 
                 Bacterial- 
                   Homo sapiens  (Human) 
                 MFRRPVLQVLRQFVRHESETTTSLVLERSLNRVHLLGRVGQDP 
                 260 
               
               
                   
                   
                 SSB 
                   
                 VLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWH 
                   
               
               
                   
                   
                   
                   
                 RISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRR 
                   
               
               
                   
                   
                   
                   
                 QATTIIADNIIFLSDQTKEKE 
                   
               
               
                   
               
               
                 SSB_27 
                 SSB 
                 Viral- 
                 Enterobacteria phage T1 
                 MAKKIFTSALGTAEPYAYIAKPDYGNEERGFGNPRGVYKVDLT 
                 261 
               
               
                   
                   
                 SSB 
                 (Bacteriophage T1) 
                 IPNKDPRCQRMVDEIVKCHEEAYAAAVEEYEANPPAVARGKK 
                   
               
               
                   
                   
                   
                   
                 PLKPYEGDMPFFDNGDGTTTFKFKCYASFQDKKTKETKHINLV 
                   
               
               
                   
                   
                   
                   
                 VVDSKGKKMEDVPIIGGGSKLKVKYSLVPYKWNTAVGASVKL 
                   
               
               
                   
                   
                   
                   
                 QLESVMLVELATFGGGEDDWADEVEENGYVASGSAKASKPRD 
                   
               
               
                   
                   
                   
                   
                 EESWDEDDEESEEADEDGDF 
                   
               
               
                   
               
               
                 SSB_28 
                 SSB 
                 Bacterial- 
                 
                   Escherichia coli 
                 
                 MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWR 
                 262 
               
               
                   
                   
                 SSB 
                   
                 DKATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQL 
                   
               
               
                   
                   
                   
                   
                 RTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAG 
                   
               
               
                   
                   
                   
                   
                 GNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAPSN 
                   
               
               
                   
                   
                   
                   
                 EPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_29 
                 SSB 
                 Viral- 
                   Mycobacterium  phage PhatBacter, 
                 MSKDLQVHLVGTLTADPELRFTQGGQAVANFTVVSNERRRDA 
                 263 
               
               
                   
                   
                 SSB 
                   Mycobacterium  phage Elph10, 
                 QGNWVDGDATFLRCTIWRDAAENVAESLQKGQRVIVHGYLK 
                   
               
               
                   
                   
                   
                   Mycobacterium  phage 244, 
                 QRSFETKEGDKRTVIEVEVDEIGPSLRWATARVSKVGGNSNGG 
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Cjw1, 
                 GSSSFKEADANKWGDDDVPF 
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Phrux, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Lilac, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Phaux, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Quink, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Pumpkin, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Murphy 
                   
                   
               
               
                   
               
               
                 SSB_30 
                 SSB 
                 Viral- 
                 
                   Bacillus thuringiensis 
                 
                 MMNRVILVGRLTKDPDLRYTPNGVAVATFTLAVNRAFANQQG 
                 264 
               
               
                   
                   
                 SSB 
                 Sbt003,  Escherichia coli \′BL21- 
                 EREADFINCVIWRKQAENVANYLKKGSLAGVDGRLQTRNYEG 
                   
               
               
                   
                   
                   
                 Gold(DE3)pLysS 
                 QDGKRVYVTEVLAESVQFLEPRNGGGEQRGSFNQQPSGAGFG 
                   
               
               
                   
                   
                   
                 AG\′, Enterobacteria phage 
                 NQGSNPFGQSSNSGNQGNSGFTKNDDPFSNVGQPIDISDDDLPF 
                   
               
               
                   
                   
                   
                 HK630, Enterobacteria phage 
                   
                   
               
               
                   
                   
                   
                 lambda (Bacteriophage 
                   
                   
               
               
                   
                   
                   
                 lambda),  Escherichia coli   
                   
                   
               
               
                   
                   
                   
                 TA280,  Escherichia coli  1-176- 
                   
                   
               
               
                   
                   
                   
                 05_S3_C2,  Escherichia coli  40967 
                   
                   
               
               
                   
               
               
                 SSB_31 
                 SSB 
                 Viral- 
                   Bordetella  phage BPP-1 
                 MASVNKVILVGNLGRDPEVRYSPDGAAICNVSIATTSQWKDKA 
                 265 
               
               
                   
                   
                 SSB 
                   
                 SGERREETEWHRVVMYNRLAEIAGEYLKKGRSVYIEGRLKTR 
                   
               
               
                   
                   
                   
                   
                 KWQDKDTGADRYSTEIVADQMQMLGGRDSGGDSGGGYGGG 
                   
               
               
                   
                   
                   
                   
                 YDDAPRQQRAPAQRPAAAPQRPAPQAAPAANLADMDDDIPF 
                   
               
               
                   
               
               
                 SSB_32 
                 SSB 
                 Viral- 
                   Burkholderia  phage BcepNazgul 
                 MASVNKVILVGNLGADPEVRYLPSGDAVANIRLATTDRYKDK 
                 266 
               
               
                   
                   
                 SSB 
                   
                 ASGDIKEATEWHRVSFFGRLAEIVDEHLRKGASVYIEGRIKTRK 
                   
               
               
                   
                   
                   
                   
                 WQDQSGQDRYTTEIVADRMQMLGKPGGSRDDSGDQQQRQHG 
                   
               
               
                   
                   
                   
                   
                 GQQQRGGGRNGYADATGRAQPQRTAEQRGASGFDDMDDSIPF 
                   
               
               
                   
               
               
                 SSB_33 
                 SSB 
                 Bacterial- 
                   Photorhabdus luminescens  subsp 
                 MASRGINKVILIGNLGQDPEVRYMPNGGAVTNITLATSESWRD 
                 267 
               
               
                   
                   
                 SSB 
                   laumondii  (strain DSM 15139/ 
                 KQTGEMKEKTEWHRVVLFGKLAEVAGEYLRKGSQVYIEGSLQ 
                   
               
               
                   
                   
                   
                 CIP105565/TT01) 
                 TRKWQDQNGQERYTTEVVVNMGGTMQMLGGRAGGGSFQDS 
                   
               
               
                   
                   
                   
                   
                 QQSQGGGWGQPQQPQPQQQSQQFSGGGAPSRPAQSSAPQSNE 
                   
               
               
                   
                   
                   
                   
                 PPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_34 
                 SSB 
                 Bacterial- 
                   Photobacterium profundum  (strain 
                 MELSMASRGVNKVILVGNLGNDPEIRYMPSGSAVANITIATSES 
                 268 
               
               
                   
                   
                 SSB 
                 SS9) 
                 WRDKATGEQREKTEWHRVALFGKLAEVAGEYLRKGSQVYIE 
                   
               
               
                   
                   
                   
                   
                 GQLQTRKWQNQQGQDQYTTEVVVQGFNGVMQMLGGRQGGG 
                   
               
               
                   
                   
                   
                   
                 QQQQQQQQQGNWGKPQQPAAAPQQSVAPQQQQQAPQQPQQ 
                   
               
               
                   
                   
                   
                   
                 APQQPQQQYNEPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_35 
                 SSB 
                 Viral- 
                   Lactobacillus  phage Lc-Nu 
                 MAITMDYSQAAEGNGDIQDGVYECVINRFGFDNYKDREFIKED 
                 269 
               
               
                   
                   
                 SSB 
                   
                 LIVRNDVPQKYQNKHIFDNFYPKKDTGEYAMGYLFMIGKNAGI 
                   
               
               
                   
                   
                   
                   
                 PDHKAWSDLAAMLADFTGHAVKVTVKNEEYNGKTYPHIKKW 
                   
               
               
                   
                   
                   
                   
                 EPTAFPQIQHRWKDSKDESSSNSNPSFGTPAQTSQTNTSDPFAN 
                   
               
               
                   
                   
                   
                   
                 SGQPIDVSDDDLPF 
                   
               
               
                   
               
               
                 SSB_36 
                 SSB 
                 Bacterial- 
                   Leuconostoc mesenteroides  subsp 
                 MINRVVLIGRLTRDVELRYTQSGVAVGTFSLAVNRQFTNASGE 
                 270 
               
               
                   
                   
                 SSB 
                   mesenteroides  (strain ATCC 8293/ 
                 READFINAVIWRKAAENFANFTGKGALVAVEGRLQTRNYENN 
                   
               
               
                   
                   
                   
                 NCDO 523) 
                 AGQRVYVTEVVVDNFSLLESRAESEKRRSQNGSSASNNGADNF 
                   
               
               
                   
                   
                   
                   
                 SGSNDHSFGGNDNSFNGVDPFASASSNSNTQSSASSNSAPNPFA 
                   
               
               
                   
                   
                   
                   
                 ASGNTEIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_37 
                 SSB 
                 Viral- 
                 
                   Staphylococcus phage 
                 
                 MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGE 
                 271 
               
               
                   
                   
                 SSB 
                 3A,  Staphylococcus  phage 
                 READFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKE 
                   
               
               
                   
                   
                   
                 phi7401PVL,  Streptococcus   
                 GQRVYVTEVVADSIQFLEPKNSNDTQQDLYQQQVQQTRGQSQ 
                   
               
               
                   
                   
                   
                   pneumoniae ,  Staphylococcus   
                 YSNNKPVKDNPFANANGPIEENDDDLPF 
                   
               
               
                   
                   
                   
                   aureus  (strain NCTC 
                   
                   
               
               
                   
                   
                   
                 8325),  Staphylococcus  phage 
                   
                   
               
               
                   
                   
                   
                 Phi12,  Staphylococcus   
                   
                   
               
               
                   
                   
                   
                   aureus ,  Staphylococcus  phage 
                   
                   
               
               
                   
                   
                   
                 47,  Staphylococcus  phage tp310-2 
                   
                   
               
               
                   
               
               
                 SSB_38 
                 SSB 
                 Viral- 
                   Escherichia  phage Tls 
                 MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWR 
                 272 
               
               
                   
                   
                 SSB 
                   
                 DKQTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQ 
                   
               
               
                   
                   
                   
                   
                 LRTRSWEDNGITRYVTEILVKTTGTMQMLGSAPQQNAQVQPQ 
                   
               
               
                   
                   
                   
                   
                 PQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQPPEGEDY 
                   
               
               
                   
                   
                   
                   
                 GFSDDIPF 
                   
               
               
                   
               
               
                 SSB_39 
                 SSB 
                 Bacterial- 
                   Leifsonia xyli  subsp  xyli , 
                 MAGETVITVVGNLTSDPELRYTQNGLAVANFTIASTPRTFDRQ 
                 273 
               
               
                   
                   
                 SSB 
                   Leifsonia   xyli  subsp  xyli   
                 ANEWKDGEALFLRASVWRDFAEHVAGSLTKGSRVIAQGRLKQ 
                   
               
               
                   
                   
                   
                 (strain CTCB07) 
                 RSYETKEGEKRTSIELEIDEIGPSLRYATAQVTRAQSSRGPGG 
                   
               
               
                   
                   
                   
                   
                 PGGFGGGAPAVEEPWAATVPADPSAGTDVWNTPGAYNDETPF 
                   
               
               
                   
               
               
                 SSB_40 
                 SSB 
                 Bacterial- 
                 
                   Legionella pneumophila 
                 
                 MISMARGINKVILVGNVGADPDVRYLPNGNAVTTLSVATSET 
                 274 
               
               
                   
                   
                 SSB 
                   
                 WKDKTTGEKQDRTEWHRVVCFNRLGEIAGEYIRKGSKLYVEG 
                   
               
               
                   
                   
                   
                   
                 SLRTRKWQDQQGQDRYTTEIVASDIQMLDSKGSSATNYDDMP 
                   
               
               
                   
                   
                   
                   
                 SFQGTSTPQQASTKNQATPTSTAQDAFDELDDDIPF 
                   
               
               
                   
               
               
                 SSB_41 
                 SSB 
                 Bacterial- 
                   Nocardia farcinica  (strain IFM 
                 MAGDTVITVIGNLTADPELRFTPAGQAVANFTVASTPRVFDRN 
                 275 
               
               
                   
                   
                 SSB 
                 10152) 
                 TNEWKDGEALFLRCNIWREAAENVAESLTRGARVIVSGRLKQ 
                   
               
               
                   
                   
                   
                   
                 RSYETREGEKRTVVELEVDEVGPSLRYATAKVNKASRGGGGG 
                   
               
               
                   
                   
                   
                   
                 GGFGGGGGGGYASDRSGGGGSRSGAAEDDPWGSAPAAGSFG 
                   
               
               
                   
                   
                   
                   
                 GGRMDDEPPF 
                   
               
               
                   
               
               
                 SSB_42 
                 SSB 
                 Viral- 
                   Streptococcus  phage Sfi21 
                 MINNVVLVGRMTRDAELRYTPSNVAVATFSLAVNRNFKGANG 
                 276 
               
               
                   
                   
                 SSB 
                   
                 ERETDFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQ 
                   
               
               
                   
                   
                   
                   
                 QGQRVYVTEVVADNFQMLESRAAREGHSGGSYNVGGFDNSN 
                   
               
               
                   
                   
                   
                   
                 SFGGGASTGGSFGESQPAQSTPNFGRDESPFGNSNPMDISDDDL 
                   
               
               
                   
                   
                   
                   
                 PF 
                   
               
               
                   
               
               
                 SSB_43 
                 SSB 
                 Bacterial- 
                   Campylobacter coli  80352 
                 MFNKVVEVGNLTRDIEMRYGQNGNAIGASAIAVTRRFTTNGER 
                 277 
               
               
                   
                   
                 SSB 
                   
                 REETCFIDISFYGRTAEVANQYLSKGSKVLIEGRERFEQWNDQN 
                   
               
               
                   
                   
                   
                   
                 GQMRSKHSVQVENMEMLGNNPQQGGNFNNGGNNYGANNNY 
                   
               
               
                   
                   
                   
                   
                 SNYENQSYDPYMSENNFKKAPQQAQTKTQNPNQNQEKIKEID 
                   
               
               
                   
                   
                   
                   
                 VDAYDSDDTDLPF 
                   
               
               
                   
               
               
                 SSB_44 
                 SSB 
                 Viral- 
                   Staphylococcus  phage 92 
                 MLNRVVLVGRLTKDPEYRTAPNGVSVTTFTIAVNRTFTNAQGE 
                 278 
               
               
                   
                   
                 SSB 
                   
                 READFINCVTFRKQAENVKNYLSKGSLAGVDGRLQTRNYENK 
                   
               
               
                   
                   
                   
                   
                 DGQRVYVTEVVADSVQFLEPKNSNQQNNQQHNEQTQTGNNPF 
                   
               
               
                   
                   
                   
                   
                 DNTTAITDDDLPF 
                   
               
               
                   
               
               
                 SSB_45 
                 SSB 
                 Bacterial- 
                   Pelobacter propionicus  (strain 
                 MASLNKVMLIGNLGRDPEVRYTASGQAVASFNLATTEKFKNR 
                 279 
               
               
                   
                   
                 SSB 
                 DSM 2379/NBRC 103807/ 
                 NGEWEERTEWHRVTLWARLAEIAGEYESKGKTVYIEGREQTR 
                   
               
               
                   
                   
                   
                 OttBd1) 
                 EYEKDGIKRYTTEIVGEKMQMLSPKGERRSSGDSYSPAPAGTS 
                   
               
               
                   
                   
                   
                   
                 GGGYEPPPFQDDDIPF 
                   
               
               
                   
               
               
                 SSB_46 
                 SSB 
                 Bacterial- 
                   Clostridium beijerinckii  (strain 
                 MNKVVLIGRLTKDPELRFTPGSGAAVTTLTLAVDKYNTKTGQR 
                 280 
               
               
                   
                   
                 SSB 
                 ATCC 51743/NCIMB 8052) 
                 EADFVPVVVWGKQAESTANYMSKGSQVAISGRIQTRSYDAKD 
                   
               
               
                   
                   
                   
                 ( Clostridium acetobutylicum ) 
                 GTKRYVTEVVADQFGGVEFLGSKGSNSSGNSFGNSNEYSAPAN 
                   
               
               
                   
                   
                   
                   
                 DAFSGGFEEDITPVDDGDMPF 
                   
               
               
                   
               
               
                 SSB_47 
                 SSB 
                 Bacterial- 
                   Sodalis glossinidius  (strain 
                 MASRGVNKVILVGNLGQDPEVRHMPNGGAVANITLATSESWR 
                 281 
               
               
                   
                   
                 SSB 
                   morsitans ) 
                 DKQTGETKEKTEWHRVVLFGKLAEVAGEYLRKGSQVYIEGSL 
                   
               
               
                   
                   
                   
                   
                 QTRKWQDQSGQDRYTTEVVVNVGGTMQMLGGRQGGAAASG 
                   
               
               
                   
                   
                   
                   
                 GQQSGWGLPQQPQGNNAQFSGGNAPASRRAQNSAPAPSNEPP 
                   
               
               
                   
                   
                   
                   
                 MDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_48 
                 SSB 
                 Bacterial- 
                 
                   Xanthobacter autotrophicus 
                 
                 MAGSVNKVILVGNLGRDPEIKSEQNGGRVCNLSVATSENWRD 
                 282 
               
               
                   
                   
                 SSB 
                 (strain ATCC BAA-1158/Py2) 
                 KASGERRERTEWHRVVIFNENLAGVAERFLKKGSKVYIEGQLE 
                   
               
               
                   
                   
                   
                   
                 TRKYEKDGRETYTTEIVLRPYRGELTLLDGRGEGAGAGGDDY 
                   
               
               
                   
                   
                   
                   
                 AAGSDFGSASPMGGGYGGGSGGSRRMSGAPAGSSGGVAGGG 
                   
               
               
                   
                   
                   
                   
                 RPAADLDDEIPF 
                   
               
               
                   
               
               
                 SSB_49 
                 SSB 
                 Viral- 
                   Clostridium  phage phiC2, 
                 MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGN 
                 283 
               
               
                   
                   
                 SSB 
                 
                   Peptoclostridium difficile 
                 
                 KITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDEN 
                   
               
               
                   
                   
                   
                 E15,  Clostridium  phage 
                 GCWKSITKVNVNALELLSSKNDNNAKQEYVPPGLDPQGFQAID 
                   
               
               
                   
                   
                   
                 phiMMP03,  Peptoclostridium   
                 DDDIPF 
                   
               
               
                   
                   
                   
                   difficile  ( Clostridium   
                   
                   
               
               
                   
                   
                   
                   difficile ) 
                   
                   
               
               
                   
               
               
                 SSB_50 
                 SSB 
                 Viral- 
                 Enterobacteria phage HK022 
                 MAQRGVNKVILIGTLGQDPEIRYIPNGGTVGRLSIATNESWRDK 
                 284 
               
               
                   
                   
                 SSB 
                 (Bacteriophage HK022) 
                 QTGQQKEQTEWHRVVLEGKLAEIASEYLRKGSQVYIEGKLKTR 
                   
               
               
                   
                   
                   
                   
                 KWTDDAGVERYTTEIIVSQGGTMQMIGARRDDSQFSNGWGQS 
                   
               
               
                   
                   
                   
                   
                 NQPQNHQQYSGGSKPQSNANSEPPMDFEDDIPF 
                   
               
               
                   
               
               
                 SSB_51 
                 SSB 
                 Viral- 
                   Mycobacterium  phage Wildcat 
                 MSKDLQVHLVGTLTADPELRFTQGGQAVANFTVVSNERRRDA 
                 285 
               
               
                   
                   
                 SSB 
                   
                 QGNWVDGDATFLRCTIWRDAAENVAESLQKGQRVIVHGYLK 
                   
               
               
                   
                   
                   
                   
                 QRSFETKEGDKRTVIEVEVDEIGPSLRWATARVSKVGGNSNGG 
                   
               
               
                   
                   
                   
                   
                 GSSSFKEADANKWGDDEPPF 
                   
               
               
                   
               
               
                 SSB_52 
                 SSB 
                 Viral- 
                   Streptococcus  phage MM1 
                 MINNVVLVGRLTRDAELRYTQSNIAVATFTLAVNRPFKNEAGE 
                 286 
               
               
                   
                   
                 SSB 
                 1998,  Streptococcus pneumoniae , 
                 READFINCVIWRQLAENLANWAKKGSLIGVTGVIQTRSYDNQQ 
                   
               
               
                   
                   
                   
                   Streptococcus  phage MM1 
                 GQRVYVTEVVASNFQLLESRNSQQNNQGHQDHHGGYQQQGY 
                   
               
               
                   
                   
                   
                   
                 SNQGSSFQNGNSYGQQGSFVEGNTTNLVPDFTRDNNPFGRPTN 
                   
               
               
                   
                   
                   
                   
                 PLDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_53 
                 SSB 
                 Viral- 
                   Lactococcus  phage c2 
                 MSINNVTLVGRLVRDPELKNTAQGIANVSFTLAVNRNYKNDQ 
                 287 
               
               
                   
                   
                 SSB 
                   
                 GQREADFINVVIWRKQAELLAQYATKGALIGITGRIQTRNYEN 
                   
               
               
                   
                   
                   
                   
                 QQGQRIYVTEVVADNFQLLESRGAQQGGQQQQQNQGYGQQQ 
                   
               
               
                   
                   
                   
                   
                 NQRPQGNYQSNPRNNQQRQNQPDPFRGSPMEISDDDLPF 
                   
               
               
                   
               
               
                 SSB_54 
                 SSB 
                 Bacterial- 
                   Salinispora tropica  (strain 
                 MAGDTIITVIGNLTDDPELRFTPSGAAVAKFRVASTPRFFDKSS 
                 288 
               
               
                   
                   
                 SSB 
                 ATCC BAA-916/DSM 44818/CNB- 
                 SEWKDGEPLFLSCTVWRQAAEHVAESLQRGTRVIVSGRLRQRS 
                   
               
               
                   
                   
                   
                 440) 
                 YETREGEKRTVIELEVDEIGPSERYATAKVQKMSRSGGGGFGG 
                   
               
               
                   
                   
                   
                   
                 GGGQGGGGNFDDPWASAAPAPAPSRGGSGGGNFDEEPPF 
                   
               
               
                   
               
               
                 SSB_55 
                 SSB 
                 Bacterial- 
                   Stigmatella aurantiaca  (strain 
                 MAGGVNKVILIGNLGADPEVRFTPGGQAVANFRIATSDSWTDK 
                 289 
               
               
                   
                   
                 SSB 
                 DW4/3-1) 
                 NGQKQERTEWHRIVVWGKLAELCGEYLKKGRQCFVEGRLQT 
                   
               
               
                   
                   
                   
                   
                 REWTDKENRKNYTTEVVASSVTFLGGRDAGEGSGMGSRRGG 
                   
               
               
                   
                   
                   
                   
                 GASSRGGEPDYGAPPPGMDDGMNQGGSGDDDIPF 
                   
               
               
                   
               
               
                 SSB_56 
                 SSB 
                 Viral- 
                   Listeria welshimeri  serovar 6b 
                 MMNRVVLVGRLTKDPELRYTPAGVAVATFTLAVNRTFTNQQG 
                 290 
               
               
                   
                   
                 SSB 
                 (strain ATCC 35897/DSM 20650/ 
                 EREADFINCVVWRKPAENVANFLKKGSMAGVDGRVQTRNYE 
                   
               
               
                   
                   
                   
                 SLCC5334),  Listeria  phage PSA 
                 GNDGKRVYVTEIVAESVQFLEPRNSNGGGGNNYQGGNNNNY 
                   
               
               
                   
                   
                   
                   
                 NNGGSNSGQAPTNNGGFGQDQQQSQNQNYQSTNNDPFASDG 
                   
               
               
                   
                   
                   
                   
                 KPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_57 
                 SSB 
                 Bacterial- 
                   Gramella forsetii  (strain 
                 MTGTLNKVMLIGHTGDDVKMHYFEGGGSIGRFPLATNESYTN 
                 291 
               
               
                   
                   
                 SSB 
                 KT0803) 
                 RTTGERVTNTEWHNVVVRNKAAEVCEKYLKKGDKVYIEGRIK 
                   
               
               
                   
                   
                   
                   
                 TRKWQDDAGNEKYSTEVHCTEFTELTPKNESNAEPSGKSQASG 
                   
               
               
                   
                   
                   
                   
                 NTSAKPKSDNFANKNEFYSQDEEEDDLPF 
                   
               
               
                   
               
               
                 SSB_58 
                 SSB 
                 Viral- 
                   Staphylococcus  phage CNPH82 
                 MTNLTILTGRITKDLELKQAGQTQVTNFSMAVDNPFKKDDTSF 
                 292 
               
               
                   
                   
                 SSB 
                   
                 EDIVAFGKTAQLLNDYCGKGSKVLIEGNEKQDRFQDKEGNNRS 
                   
               
               
                   
                   
                   
                   
                 VVRVIANRIEFLDSKGSNQSNNQSPKRGQAPAGNNPFANGTDIE 
                   
               
               
                   
                   
                   
                   
                 NSELPF 
                   
               
               
                   
               
               
                 SSB_59 
                 SSB 
                 Viral- 
                   Staphylococcus  phage Pvl108 
                 MKITGQAQFTKETNQEKFYNGSAGFQAGEFTVKVKNIEFNDRE 
                 293 
               
               
                   
                   
                 SSB 
                   
                 NRYFTIVFENDEGKQYKHNQFVPPYKYDFQEKQLIELVTRLGIK 
                   
               
               
                   
                   
                   
                   
                 LNLPSLDFDTNDLIGKFCHLVLKWKFNKDEGKYFTDFSFIKPYK 
                   
               
               
                   
                   
                   
                   
                 KGDDVVNKPIPKTDKQKAEENNGAQQQTSMSQQSNPFESSGQ 
                   
               
               
                   
                   
                   
                   
                 EGYDDQDLAF 
                   
               
               
                   
               
               
                 SSB_60 
                 SSB 
                 Viral- 
                   Prochlorococcus  phage P-SSP7 
                 MNHCVLEVEVIQAPTIRYTQDNQTPIAEMEVRFDALRVDTAPG 
                 294 
               
               
                   
                   
                 SSB 
                   
                 QLKVVGWGNLAQDLQNRVQVGQRLVIEGRLRMNTVPRQDGT 
                   
               
               
                   
                   
                   
                   
                 KEKRAEFTLAREHSLSGQAGQPASSPERPTSTTTNQTSRPIPIA 
                   
               
               
                   
                   
                   
                   
                 ETPTPKPATTAEPEAASWNSAPLVPDTDDIPF 
                   
               
               
                   
               
               
                 SSB_61 
                 SSB 
                 Bacterial- 
                   Vibrio cholerae  1587 
                 MASRGVNKVILIGNLGQDPEVRYMPSGGAVANITIATSETWRD 
                 295 
               
               
                   
                   
                 SSB 
                   
                 KATGEQKEKTEWHRVTLYGKLAEVAGEYLRKGSQVYIEGQLQ 
                   
               
               
                   
                   
                   
                   
                 TRKWQDQSGQDRYSTEVVVQGYNGIMQMLGGRTQQGGMPA 
                   
               
               
                   
                   
                   
                   
                 QGGMNAPAQQGSWGQPQQPAKQHQPMQQSAPQQYSQPQYNE 
                   
               
               
                   
                   
                   
                   
                 PPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_62 
                 SSB 
                 Bacterial- 
                   Bacteroides caccae  ATCC 43185 
                 MSVNRVILIGNVGQDPRVKYFDTGSAVATFPLATTDRGYTLQN 
                 296 
               
               
                   
                   
                 SSB 
                   
                 GTQIPERTEWHNIVASNRLAEIVDKYVHKGDKLYLEGKIRTRS 
                   
               
               
                   
                   
                   
                   
                 YSDQSGAMRYITEIYVDNMEMLTPKGAGQGAGTSAQQGAATP 
                   
               
               
                   
                   
                   
                   
                 SQQPQQMQQNQQPQQSQAQPVQDNPADDLPF 
                   
               
               
                   
               
               
                 SSB_63 
                 SSB 
                 Bacterial- 
                   Elusimicrobium minutum  (strain 
                 MASQIRLPEQNLVLLTGRLTRDANTAFTQKGAAVSRFDIAVNR 
                 297 
               
               
                   
                   
                 SSB 
                 Pei191) 
                 RYMDANGSWQDETTFVPVTLWGPAAERSKDRLKKGVPVHVE 
                   
               
               
                   
                   
                   
                   
                 GRLVLNEYTDKNGVAHKNEQVNCRRIQILQSAFSESASGSGAS 
                   
               
               
                   
                   
                   
                   
                 FNDTPVDNEAIDDDVPF 
                   
               
               
                   
               
               
                 SSB_64 
                 SSB 
                 Bacterial- 
                 
                   Methylobacterium nodulans 
                 
                 MAGSVNKVILVGNLGRDPETRRETSGDPVVNLRLATSESWKD 
                 298 
               
               
                   
                   
                 SSB 
                 (strain LMG 21967/CNCM I- 
                 KATGERKEKTEWHSVVIYNENLARVAEQYLRKGSKVYVEGQL 
                   
               
               
                   
                   
                   
                 2342/ORS 2060) 
                 QTRKWQDQSGVEKYTTEVVLQRFRGELTILDGRGGGETGAMD 
                   
               
               
                   
                   
                   
                   
                 EMGGGQISRGGDFGGDRSSGGERRPAPAGGSQKRYDDLDDDIP 
                   
               
               
                   
                   
                   
                   
                 F 
                   
               
               
                   
               
               
                 SSB_65 
                 SSB 
                 Viral- 
                   Yersinia  phage YpsP-G,  Yersinia   
                 MAVNKIILLGNVGNYPEVNYTGSGTAVAKFSVATTEKWKDQS 
                 299 
               
               
                   
                   
                 SSB 
                 phage phiA1122 
                 GEKKEKTNWHRCVVFGKRAEVVGEYVRKGSQLYIEGSMEYGS 
                   
               
               
                   
                   
                   
                   
                 YDKDGVTMYTADVHVKDFQFIGGKREDGGNAGGGQSGGWG 
                   
               
               
                   
                   
                   
                   
                 QPQQTQQQRQPASQPTNTATLKPEGQPKQRQMSQAERMLAQQ 
                   
               
               
                   
                   
                   
                   
                 AAQAQQQSSEPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_66 
                 SSB 
                 Bacterial- 
                   Clostridium botulinum  C str 
                 MNRVVLVGRLTKDPELRFTPGAGKAVATFTLAVNRRFKSQGQ 
                 300 
               
               
                   
                   
                 SSB 
                 Eklund 
                 PDADFLPIVVWGKQAENTANYVGKGSQVGVSGAIHTRSYEAK 
                   
               
               
                   
                   
                   
                   
                 DGTRRYVTEIVADEVQFLDSRNAVSRTEPRSTGMNEFDSSNND 
                   
               
               
                   
                   
                   
                   
                 NDFNQSYDEEITPIDDGDIPF 
                   
               
               
                   
               
               
                 SSB_67 
                 SSB 
                 Bacterial- 
                   Ureaplasma urealyticum  serovar 
                 MNKVILIGNLVRDPEARQIPSGRLVTNFTVAVNDNIPNANANFI 
                 301 
               
               
                   
                   
                 SSB 
                 12 str ATCC 33696 
                 RCVAWNNQANFLTTYLKKGDAIAIEGRIVSRSYVDNNGKTNY 
                   
               
               
                   
                   
                   
                   
                 VTEVYADQVQSLSRRNQNANDHNNDKVNVDTMMGAYASINT 
                   
               
               
                   
                   
                   
                   
                 DAAFSSNQPQTNFQSTTSNSNKNDDEEDEITSWINLDDDLE 
                   
               
               
                   
               
               
                 SSB_68 
                 SSB 
                 Bacterial- 
                   Mycobacterium marinum  (strain 
                 MAGDTTITVVGNLTADPELRFTPSGAAVANFTVASTPRIYDRQS 
                 302 
               
               
                   
                   
                 SSB 
                 ATCC BAA-535/M) 
                 GEWKDGEALFLRCNIWREAAENVAESLTRGARVIVSGRLKQRS 
                   
               
               
                   
                   
                   
                   
                 FETREGEKRTVVEVEVEEIGPSERYATAKVNKASRGGGGGGFG 
                   
               
               
                   
                   
                   
                   
                 GGGGGGGGGGARQAPAQASSPAGGDDPWGSAPASGSFGGDD 
                   
               
               
                   
                   
                   
                   
                 EPPF 
                   
               
               
                   
               
               
                 SSB_69 
                 SSB 
                 Bacterial- 
                 
                   Oligotropha carboxidovorans 
                 
                 MAGSVNKVILVGNLGADPDVRRTQDGRPIVNESIATSDTWRDK 
                 303 
               
               
                   
                   
                 SSB 
                 (strain ATCC 49405/DSM 1227/ 
                 ATGERKEKTEWHRVVIFNEGLCRVAEQYLKKGAKVYIEGALQ 
                   
               
               
                   
                   
                   
                 KCTC 32145/OM5) 
                 TRKWQDKDGKDKYSTEVVLQGFNSTLTMLDGRSGGGGGSFA 
                   
               
               
                   
                   
                   
                   
                 GDDAGSSFGSSGSSQRRSLPPSGGRDDMNDDIPF 
                   
               
               
                   
               
               
                 SSB_70 
                 SSB 
                 Bacterial- 
                   Clostridium botulinum  (strain 
                 MNKVVLIGRLTKDPELRFTPGAGTAVTTLTLAVDKYNSKSGQK 
                 304 
               
               
                   
                   
                 SSB 
                 Eklund 17B/Type B) 
                 EADFVPVVVWGKQAESTANYMSKGSQMAISGRIQTRNYEAKD 
                   
               
               
                   
                   
                   
                   
                 GTKRYVTEVVATEVQFLSKSNASGNVGTNYGNNTEYSSSNNPF 
                   
               
               
                   
                   
                   
                   
                 DGMNFEEDITPVDDGDMPF 
                   
               
               
                   
               
               
                 SSB_71 
                 SSB 
                 Bacterial- 
                   Collinsella stercoris  DSM 13279 
                 MSINRVNISGNLTRDPELRATSSGTQVLSFGVAVNDRRRNPQT 
                 305 
               
               
                   
                   
                 SSB 
                   
                 GDWEDYPNFVDCTMFGTRAEAVKRYLSKGSKVAIEGKLRYSS 
                   
               
               
                   
                   
                   
                   
                 WERDGQRRSKLEVIVDEIEFMSRGQQGEGGGYAPAPSYGQQG 
                   
               
               
                   
                   
                   
                   
                 GYAPAPAPQQAPAPMAAPVPPAVDVYDEDIPF 
                   
               
               
                   
               
               
                 SSB_72 
                 SSB 
                 Bacterial- 
                   Haemophilus parasuis  serovar 5 
                 MAGVNKVIIVGNLGNDPDMRTMPNGDAVATLSVATSESWND 
                 306 
               
               
                   
                   
                 SSB 
                 (strain SH0165) 
                 KMTGERREVTEWHRIVFFRRQAEVAGQYLRKGSKVYVEGKLK 
                   
               
               
                   
                   
                   
                   
                 TRKWQDQNGQDRYTTEIQGDVLQMLDSRSGDQGGGWNQTQT 
                   
               
               
                   
                   
                   
                   
                 NYNQDGYTDSYAQNNNFNGGNATRPQPAQKPAAQAEPPMDN 
                   
               
               
                   
                   
                   
                   
                 FDDDIPF 
                   
               
               
                   
               
               
                 SSB_73 
                 SSB 
                 Bacterial- 
                 
                   Agrobacterium rhizogenes 
                 
                 MAGSVNKVILVGNEGADPEIRRTQDGRPIANLNIATSETWRDR 
                 307 
               
               
                   
                   
                 SSB 
                   
                 NSGERKEKTEWHRVVIFNEGLCKVAEQYLKKGAKVYIEGALQ 
                   
               
               
                   
                   
                   
                   
                 TRKWQDQNGQDKYSTEIVLQGFNSTLTMLDGRGEGGGGGNR 
                   
               
               
                   
                   
                   
                   
                 GGGGGDFGGGDYGGDDYGQPAPSSGGGRSAGASRGAPASSGG 
                   
               
               
                   
                   
                   
                   
                 GSNFSRDLDDDIPF 
                   
               
               
                   
               
               
                 SSB_74 
                 SSB 
                 Viral- 
                   Salmonella  phage 553e 
                 MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTVATSEQWRD 
                 308 
               
               
                   
                   
                 SSB 
                   
                 KQTGEQKEQTEWHRVAVVGKLAEVVGQYVKKGDQVYFEGM 
                   
               
               
                   
                   
                   
                   
                 LRTRKWQDQTGQDRYTTEINVGINGVMQMLGGTGDSKQQAA 
                   
               
               
                   
                   
                   
                   
                 DRQSQKPQQQPSPTQHNEPPMDFDDDIPFAPVTLPFPRHAIHAI 
                   
               
               
                   
               
               
                 SSB_75 
                 SSB 
                 Bacterial- 
                 [ Clostridium ]  methylpentosum   
                 MLNKVILMGRLTADPEVRQTPNGISVLSFSIAVDRNYSKAEKK 
                 309 
               
               
                   
                   
                 SSB 
                 DSM 5476 
                 TDFINLVAWRQTAEFIGRFFTKGQMIAVEGSIQTRNYEDKTGA 
                   
               
               
                   
                   
                   
                   
                 KRTAFEVVVDQAHFTGGKSENPVRTTQPSYQQPPKFEEPAQNG 
                   
               
               
                   
                   
                   
                   
                 ESFSVGDFNDFDGFEEIGTDDGDLPF 
                   
               
               
                   
               
               
                 SSB_76 
                 SSB 
                 Bacterial- 
                   Helicobacter pullorum  MIT 98- 
                 MFNKVILVGNLTRDVELRYLPSGAALARLNLATNRRYKKQDG 
                 310 
               
               
                   
                   
                 SSB 
                 5489 
                 TQAEEVCFIDVNLFGRTAEVANQYLKKGSQVLIEGRLVLESWT 
                   
               
               
                   
                   
                   
                   
                 DNTGAKRTKHSITAESMQMLGQRQSTQEENHDYGAGDYNNY 
                   
               
               
                   
                   
                   
                   
                 QEYEKPAYTASAAPKAQPVQKEPELPVIDINDDEIPF 
                   
               
               
                   
               
               
                 SSB_77 
                 SSB 
                 Bacterial- 
                   Persephonella marina  (strain 
                 MLNKVFLIGRLTRDPEIRELPSGSQVTSFSVAVNRSYRVNNEWK 
                 311 
               
               
                   
                   
                 SSB 
                 DSM 14350/EX-H1) 
                 EETYFFDVEAFGYLAERLGKQLNKGTQILIEGQLRQDRWETAG 
                   
               
               
                   
                   
                   
                   
                 GDKRTKVKIVADKVSILSPKGEKAEKSEEEPELDIENSIEDFSS 
                   
               
               
                   
                   
                   
                   
                 DEDVPF 
                   
               
               
                   
               
               
                 SSB_78 
                 SSB 
                 Bacterial- 
                   Brevibacillus brevis  (strain 47/ 
                 MNKVILIGNLTKDPELRYTPNGVAVATFTVAVNRPRTNQAGER 
                 312 
               
               
                   
                   
                 SSB 
                 JCM 6285/ NBRC 100599) 
                 ETDFINIVAWQKLADLCASYLRKGRQAAIEGRMQTRSYDNKE 
                   
               
               
                   
                   
                   
                   
                 GKKVYVTEVVAENVQFLGGRGNESGGDNPGYDPGPGMGGGN 
                   
               
               
                   
                   
                   
                   
                 KPSGQRNNDYDPFGDPFASAGKPINISDDDLPF 
                   
               
               
                   
               
               
                 SSB_79 
                 SSB 
                 Bacterial- 
                   Corynebacterium striatum  ATCC 
                 MAIDIITITGGLPRDAELRFTKSGKAVTSFTLANSDNKFDQEQN 
                 313 
               
               
                   
                   
                 SSB 
                 6940 
                 QWVKTRSMYLDVTIWDESTERKQNPVQWARLASELKQGDQV 
                   
               
               
                   
                   
                   
                   
                 AVKGKLTTRTWETDGGEKRSKMEFLATSFYRMPTTQGAPNGQ 
                   
               
               
                   
                   
                   
                   
                 QAQQNITQGLGAQAAGDPWNGAPQGGFSGADANPPF 
                   
               
               
                   
               
               
                 SSB_80 
                 SSB 
                 Bacterial- 
                   Cryptobacterium curtum  (strain 
                 MSSINRVFITGNLSRDPELRTTASGSAVLSFGVAVVDSVKNQQT 
                 314 
               
               
                   
                   
                 SSB 
                 ATCC 700683/ DSM 15641/12- 
                 GEWEDRPNWVECTLFGSRAQAISGYLHKGSKVAIDGRLHWSQ 
                   
               
               
                   
                   
                   
                 3) 
                 WERNGEKRSKLEVIINDIQFLSPREGAQGAPQQPQYQQPAPTYQ 
                   
               
               
                   
                   
                   
                   
                 QPAPTYQQTAQQPPQQPQTAPQYASASVYDEDIPF 
                   
               
               
                   
               
               
                 SSB_81 
                 SSB 
                 Viral- 
                   Listeria  phage A500 
                 MRGGWFCMMNRVVLVGRLTKDPELRYTPSGVAVATFTLAVN 
                 315 
               
               
                   
                   
                 SSB 
                 (Bacteriophage A500) 
                 RTFTNQQGEREADFINCVVWRKPAENVANYLKKGSLAGVDGR 
                   
               
               
                   
                   
                   
                   
                 VQTRNYEGQDGKRVYVTEIVAESVQFLEPRNNSGQHQDNNNN 
                   
               
               
                   
                   
                   
                   
                 SYNQAPANNGFNQNQQQSQSYQTTNNDPFANDGKPIDISDDDL 
                   
               
               
                   
                   
                   
                   
                 PF 
                   
               
               
                   
               
               
                 SSB_82 
                 SSB 
                 Viral- 
                   Lactobacillus  prophage 
                 MINNVVLVGRLTRDPDLRTTGSGISVATFTLAVDRQYTNSQGE 
                 316 
               
               
                   
                   
                 SSB 
                 Lj928,  Lactobacillus johnsonii   
                 RGADFINCVIWRKAAENFANETSKGSEVGIQGRIQTRTYDDKD 
                   
               
               
                   
                   
                   
                 (strain CNCM 1-12250/La1/ 
                 GKRVYVTEVIVDNFSLLESRRDRENRQTNGGNFAPQGGNAPST 
                   
               
               
                   
                   
                   
                 NCC 533) 
                 NNFGGSSAPSMNNAPASGESNKPQDPFADSGSTIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_83 
                 SSB 
                 Bacterial- 
                   Vibrio cholerae  (strain 
                 MITEQNMASRGVNKVILIGNLGQDPEVRYMPSGGAVANITIAT 
                 317 
               
               
                   
                   
                 SSB 
                 MO10), Vibrio 
                 SETWRDKATGEQKEKTEWHRVTLYGKLAEVAGEYLRKGSQV 
                   
               
               
                   
                   
                   
                   cholerae ,  Providencia   
                 YIEGQLQTRKWQDQSGQDRYSTEVVVQGYNGIMQMLGGRAQ 
                   
               
               
                   
                   
                   
                 
                   alcalifaciens 
                 
                 QGGMPAQGGMNVPAQQGSWGQPQQPAKQHQPMQQSAPQQY 
                   
               
               
                   
                   
                   
                 Ban1,  Vibrio cholerae  Ind4 
                 SQPQYNEPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_84 
                 SSB 
                 Bacterial- 
                   Fusobacterium mortiferum  ATCC 
                 MNVVVLVGRLTRDPELKFGQSGKAYSRFSLAVDRPFSKGEADF 
                 318 
               
               
                   
                   
                 SSB 
                 9817 
                 INCVAFGKTAELIGEYLRKGRKVGVNGRLQMNRFEMNGEKRT 
                   
               
               
                   
                   
                   
                   
                 SYDVLVEAIEFLESKGSGDSMGGYEPEYSSPTPKSSAKEVEEI 
                   
               
               
                   
                   
                   
                   
                 PYEDDDEFPF 
                   
               
               
                   
               
               
                 SSB_85 
                 SSB 
                 Viral- 
                   Burkholderia  phage BcepNY3 
                 MASVNKVILVGNLGADPETRYLPSGDAISNIRLATTDRYKDKA 
                 319 
               
               
                   
                   
                 SSB 
                   
                 SGEFEKEVTEWHRVAFFGRLAEIVDEHLRKGASVYIEGRIKTRK 
                   
               
               
                   
                   
                   
                   
                 WQDQSGQDRYTTEIVADRMQMLGKPSGSRDDGGGERQQRAP 
                   
               
               
                   
                   
                   
                   
                 QQQQQQRGQRNGYADATGRGQPQRDAQQRPPAGGGFDEMD 
                   
               
               
                   
                   
                   
                   
                 DDIPF 
                   
               
               
                   
               
               
                 SSB_86 
                 SSB 
                 Bacterial- 
                   Agathobacter rectalis  (strain 
                 MNKVILMGRLTRDAEIRYAQGDNSLAIARFSLAVDRRYSKNAE 
                 320 
               
               
                   
                   
                 SSB 
                 ATCC 33656/DSM 3377/JCM 
                 EQSTDFINCVAFGKIAEFFERFGRKGTKFVVEGRIQTGSYTNKD 
                   
               
               
                   
                   
                   
                 17463/KCTC5835/VPI 0990) 
                 GQKVYTTDVVVENAEFAESKSAASGNAGGFAPADRPAPSQAA 
                   
               
               
                   
                   
                   
                 ( Eubacterium rectale ) 
                 GDGFMNIPDGIDEELPFN 
                   
               
               
                   
               
               
                 SSB_87 
                 SSB 
                 Viral- 
                 Cyanophage PSS2 
                 MATINILGREGKDPEVKFFDSGNCVAKFTIGDVAGRKDDPTNW 
                 321 
               
               
                   
                   
                 SSB 
                   
                 FDCEIWGKRAQLLGDTVSKGQRLMVSGDIKTETWTAKDGGNR 
                   
               
               
                   
                   
                   
                   
                 SKQVVRVSDFQYIESRGEAAGGGQATSEEIPF 
                   
               
               
                   
               
               
                 SSB_88 
                 SSB 
                 Bacterial- 
                 
                   Acinetobacter radioresistens 
                 
                 MRGVNKVILVGTLGRDPETKTFPNGGSLTQFSIATSESWTDKST 
                 322 
               
               
                   
                   
                 SSB 
                 SK82 
                 GERKEQTEWHRIVLHNREGEIAQQYLRKGSKVYIEGSLRTRQW 
                   
               
               
                   
                   
                   
                   
                 TDQNGQERYTTEIRGEQMQMLDSGRQQGDQAGAGFGGDQGY 
                   
               
               
                   
                   
                   
                   
                 GQPRFNNNQGSQGGYGNGNQQGGGFNNNNNQGGGYGNNNP 
                   
               
               
                   
                   
                   
                   
                 GGFAPKAPQSAPASQVPADLDDDLPF 
                   
               
               
                   
               
               
                 SSB_89 
                 SSB 
                 Bacterial- 
                   Enterococcus faecalis  TX0027 
                 MINQVVLVGRETKDIDERYTASGSAVGSFTLAVNRNEKNQNG 
                 323 
               
               
                   
                   
                 SSB 
                   
                 DREADFINCVIWRKPAETMANYARKGTLLGVVGRIQTRNYDN 
                   
               
               
                   
                   
                   
                   
                 QQGQRVYVTEVICESFQLLEPKSANENSNSIQTSQNDGTSVQNN 
                   
               
               
                   
                   
                   
                   
                 FEGNYATNQNKGENQQNNSQQMSFGGDVDPFAGAGNSIDISD 
                   
               
               
                   
                   
                   
                   
                 DDLPF 
                   
               
               
                   
               
               
                 SSB_90 
                 SSB 
                 Bacterial- 
                   Klebsiella pneumoniae  subsp 
                 MKVISRGQVQQVPAKRQNRGPGNSTGDGFKRQNNGLRRFIMA 
                 324 
               
               
                   
                   
                 SSB 
                   rhinoscleromatis  ATCC 13884 
                 ARGVNKVILVGYLGQDPEVRYMPNGGAVANLTLATSETWRD 
                   
               
               
                   
                   
                   
                   
                 KQTGEMRENTEWHRVVMFGKLAEVAGEYLRKGAQVYIEGQL 
                   
               
               
                   
                   
                   
                   
                 RTRNWQDDAGVTRYVTEVLVGQNGTMQMLGGRRESGVPESA 
                   
               
               
                   
                   
                   
                   
                 AQPQNPATPAQPAQAAAKSPKAKGGKKGRQDAAPSQQPPQPL 
                   
               
               
                   
                   
                   
                   
                 PDDFPPMDDDAPF 
                   
               
               
                   
               
               
                 SSB_91 
                 SSB 
                 Bacterial- 
                   Haemophilus influenzae , 
                 MRFFMAGINKVIIVGHLGNDPEIRTMPNGDAVANISVATSESW 
                 325 
               
               
                   
                   
                 SSB 
                   Haemophilus influenzae  NT127 
                 NDRNTGERREVTEWHRIVFYRRQAEICGEYERKGSQVYVEGRL 
                   
               
               
                   
                   
                   
                   
                 KTRKWQDQNGQDRYTTEIQGDVMQMLGGRNQNAGYGNDMG 
                   
               
               
                   
                   
                   
                   
                 GAPQPSYQARQTNNGGSYQSSRPAPQQSAPQAEPPMDGFDDDI 
                   
               
               
                   
                   
                   
                   
                 PF 
                   
               
               
                   
               
               
                 SSB_92 
                 SSB 
                 Bacterial- 
                 
                   Leptotrichia goodfellowii 
                 
                 MNQVLLIGRETKDPELKYSQSGKAFCRFSIAVTKEFNRNETDFF 
                 326 
               
               
                   
                   
                 SSB 
                 F0264 
                 DCVAWNKTAEIIAEYMRKGKKIAIQGRLETGSYEKEGRNIKTY 
                   
               
               
                   
                   
                   
                   
                 SIIVDKFEFVDSAGGQGQQQSSSYSQGTQPKETFADNDNDEIMD 
                   
               
               
                   
                   
                   
                   
                 DDDFPF 
                   
               
               
                   
               
               
                 SSB_93 
                 SSB 
                 Viral- 
                   Lactobacillus  phage phij11 
                 MINNVVLVGRLTRDPDLRTTGSGISVATFTLAVDRQYTNSQGE 
                 327 
               
               
                   
                   
                 SSB 
                   
                 RGADFINCVIWRKAAENFANFTSKGSLVGIQGRIQTRTYDDKD 
                   
               
               
                   
                   
                   
                   
                 GKRVYVTEVIVDNFSLLESRRDRENRQANGGNFAPQGGNAPST 
                   
               
               
                   
                   
                   
                   
                 NNFGGSSAPSMNNAPASGESNKPQDPFADSGSTIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_94 
                 SSB 
                 Bacterial- 
                   Fusobacterium ulcerans  12-1B 
                 MNLVVLTGRLTRDPELKFGQSGKAYSRFSLAVDRPFQKGEADF 
                 328 
               
               
                   
                   
                 SSB 
                   
                 INCVAFGKTAELIGEYERKGRKVGVNGRLQMNRYEANGEKRT 
                   
               
               
                   
                   
                   
                   
                 SYDVLVENIEFLEAKGSGDSAGYEPHDYAAAAPASAPKPSVKE 
                   
               
               
                   
                   
                   
                   
                 AEDVPFDDDDEFPF 
                   
               
               
                   
               
               
                 SSB_95 
                 SSB 
                 Bacterial- 
                   Pediococcus acidilactici  DSM 
                 MINRAVLVGRLTRDPELRYTSSGAAVVSFTVAVNRQFTNSQGE 
                 329 
               
               
                   
                   
                 SSB 
                 20284 
                 READFINCVMWRKAAENFANFTRKGSLVGIDGRIQTRSYENQQ 
                   
               
               
                   
                   
                   
                   
                 GQRVYVTEVVADNFSLLESRSASERRQENEGFNNGQSAPSQSS 
                   
               
               
                   
                   
                   
                   
                 AGNPFDSGQANNNGAASQPNNSNPNDPFANGGQSIDISDDDLP 
                   
               
               
                   
                   
                   
                   
                 F 
                   
               
               
                   
               
               
                 SSB_96 
                 SSB 
                 Bacterial- 
                   Desulfovibrio  sp FW1012B 
                 MAGSINKVILVGRLGQDPKLTYLASGSPVAEFSVATDESYKDR 
                 330 
               
               
                   
                   
                 SSB 
                   
                 EGNKQEKTEWHRVKVFGRSAEFCNNYLTKGRLVYIEGTLRTRS 
                   
               
               
                   
                   
                   
                   
                 WEDQQGQKRYTTEVVVTGPGHTVQGLDSRGQASEAPMGEEG 
                   
               
               
                   
                   
                   
                   
                 GFQPRRAPQQGGGGGGQGGAPRGNYGGQGQSGGSRQQPYPD 
                   
               
               
                   
                   
                   
                   
                 EDQGPAFPSEASGMDDVPF 
                   
               
               
                   
               
               
                 SSB_97 
                 SSB 
                 Bacterial- 
                   Hungatella hathewayi  DSM 13479 
                 MNRVILMGRLTRDPEVRYSQGERAMAIARYTLAVDRRGRRNQ 
                 331 
               
               
                   
                   
                 SSB 
                   
                 DGNEQTADFINCVAFDRAGEFAEKYFRQGMRVLISGRIQTGSY 
                   
               
               
                   
                   
                   
                   
                 TNKDGIKVYTTDIIVDDQEFADSKGAASGEGGGYQPTSRPAPSS 
                   
               
               
                   
                   
                   
                   
                 AIGDGFMNIPDGVEDEGLPFN 
                   
               
               
                   
               
               
                 SSB_98 
                 SSB 
                 Bacterial- 
                 
                   Streptococcus gallolyticus 
                 
                 MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQNG 
                 332 
               
               
                   
                   
                 SSB 
                 subsp  gallolyticus  TX20005 
                 EREADFINCVIWRQQAENLSNWAKKGTLIGVTGRIQTRNYENQ 
                   
               
               
                   
                   
                   
                   
                 QGQRVYVTEIVADNFQILESRATREGQSGGSYNGGFNNNNSSF 
                   
               
               
                   
                   
                   
                   
                 GGSSNDGGFSSQPSQSQTPNFGRDESPFGNSNPMDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_99 
                 SSB 
                 Bacterial- 
                 
                   Hydrogenobacter thermophilus 
                 
                 MLNKVLIIGRLTKDPSVRYLPSGNQITEFSIAYNRRYKVGDDW 
                 333 
               
               
                   
                   
                 SSB 
                 (strain DSM 6534/IAM 12695/ 
                 KEESHFFDVKAYGKLAESESTRISKGYTVVVEGRLTQDRWTDK 
                   
               
               
                   
                   
                   
                 TK-6) 
                 EGKAQSKVRIVADAVRIINKPKEDEAPEEEVIPDTYEQEAEEKL 
                   
               
               
                   
                   
                   
                   
                 WNSQDDEIPF 
                   
               
               
                   
               
               
                 SSB_100 
                 SSB 
                 Bacterial- 
                   Ruminococcus  sp SR1/5 
                 MNKVILMGRLTRDPEVRYSAGENALAIARYTLAVDRRFRRDG 
                 334 
               
               
                   
                   
                 SSB 
                   
                 EASADFISCVSFGRTAEFAEKYFRQGLKIAVTGRIQTGSYTNRE 
                   
               
               
                   
                   
                   
                   
                 GQKVYTTEVVVEDQEFAESKASSDSYAAAHPRTEAAPATSMPS 
                   
               
               
                   
                   
                   
                   
                 PSAASADGFMNIPDGIDEELPFN 
                   
               
               
                   
               
               
                 SSB_101 
                 SSB 
                 Bacterial- 
                   Serratia odorifera  DSM 4582 
                 MVLFGKLAEVAGEYLRKGSQVYIEGALQTRKWTDQAGVEKY 
                 335 
               
               
                   
                   
                 SSB 
                   
                 TTEVVVNVGGTMQMLGGRQGGGAPAGGGQAAGGQGNWGQP 
                   
               
               
                   
                   
                   
                   
                 QQPQGGNQFSGGQQSRPAQNSNAPAASSNEPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_102 
                 SSB 
                 Bacterial- 
                   Acinetobacter  sp SH024 
                 MRGVNKVILVGTLGRDPETKTFPNGGSLTQFSIATSEAWTDKN 
                 336 
               
               
                   
                   
                 SSB 
                   
                 TGERKEQTEWHRIVLHNRLGEIAQQFLRKGSKVYIEGSLRTRQ 
                   
               
               
                   
                   
                   
                   
                 WTDQNGQERYTTEIRGDQMQMLDARQQGEQGFAGGNDFNQP 
                   
               
               
                   
                   
                   
                   
                 RFNAPQQGGNGYQNNNNQGGGYGQNSGGYGSQGGFGNGGSN 
                   
               
               
                   
                   
                   
                   
                 PQAGGFAPKAPQQPASAPADLDDDLPF 
                   
               
               
                   
               
               
                 SSB_103 
                 SSB 
                 Viral- 
                   Burkholderia  phage BcepGomr 
                 MASVNKVILVGNLGADPEVRYLPSGDAVANIRLATTDRYKDK 
                 337 
               
               
                   
                   
                 SSB 
                   
                 ASGEMKEATEWHRVSFFGRLAEIVSEYLKKGSSVYLEGRIRTR 
                   
               
               
                   
                   
                   
                   
                 KWQAQDGTDRYSTEIVAEQMQMLGGRGGSMGGGGDEGGYS 
                   
               
               
                   
                   
                   
                   
                 RGEPSERSGGGGGGRAASGGGSRGGSGGGAGGGASRPSAPAG 
                   
               
               
                   
                   
                   
                   
                 GGFDEMDDDIPF 
                   
               
               
                   
               
               
                 SSB_104 
                 SSB 
                 Viral- 
                   Burkholderia  phage BcepC6B 
                 MASVNKVILVGNLGADPEVRYLPSGDAVANIRVATTDRYKDK 
                 338 
               
               
                   
                   
                 SSB 
                   
                 ESGELKEVTEWHRVSFFGRLAEIVSEYLKKGSSVYIEGRLRTRK 
                   
               
               
                   
                   
                   
                   
                 WQQDGVDRYSTEIVADQMQMLGGNGKGRTGEGEGDSESGAA 
                   
               
               
                   
                   
                   
                   
                 PETAAGAPAEGSSSKTRSRRAAPQRRASATAGNEMDDDEPFA 
                   
               
               
                   
               
               
                 SSB_105 
                 SSB 
                 Bacterial- 
                 
                   Komagataeibacter oboediens 
                 
                 MAGSVNKVILVGNEGKDPEIRNSQNGAKIVSLTLATSETWNDR 
                 339 
               
               
                   
                   
                 SSB 
                   
                 ASGERRERTEWHRVVIFNERIGDVAERFLRKGRKVYLEGTLQT 
                   
               
               
                   
                   
                   
                   
                 RKWTDQSGMERYTTEVVIDRFRGELVELDSNRGGEGGEGGGY 
                   
               
               
                   
                   
                   
                   
                 GGGPGGGGGYGGGAPRPAQAPRSTPPAGGGGGWDAPSGGSDL 
                   
               
               
                   
                   
                   
                   
                 DDEIPF 
                   
               
               
                   
               
               
                 SSB_106 
                 SSB 
                 BacterialV 
                   Peptoniphilus duerdenii  ATCC 
                 MNVVTLIGRLTRDPELRYSPSGMANVRITVAVDRGYNQQKRQ 
                 340 
               
               
                   
                   
                 SSB 
                 BAA-1640 
                 EAESQNQPTADFISCVAFGKTAELIANYFNKGNRIGLEGRIQTG 
                   
               
               
                   
                   
                   
                   
                 SYDKPDGTRVYTTDVVVNRVHFIESRSESQTYQRRPQEGAGGF 
                   
               
               
                   
                   
                   
                   
                 SAPSQNPGGFNKPPVNSSYDQFSTSEDEGEAFFPVDNEDIPF 
                   
               
               
                   
               
               
                 SSB_107 
                 SSB 
                 Bacterial- 
                 
                   Paenibacillus curdlanolyticus 
                 
                 MLNRVILIGRLTRDPELRYTPAGVAVTQFTLAVDRPFTSGGGER 
                 341 
               
               
                   
                   
                 SSB 
                 YK9 
                 EADEIPVVTWRQLAETCANYLRKGRLAAVEGRIQVRNYENNE 
                   
               
               
                   
                   
                   
                   
                 GKRVYVTEVIADNVRFLESNREGGAPREEGSYGGGNSGGGFG 
                   
               
               
                   
                   
                   
                   
                 GGSNAGGGSYGGNSGGGSRSGQNQRNDNRDPFSDDGRPIDISE 
                   
               
               
                   
                   
                   
                   
                 DDLPF 
                   
               
               
                   
               
               
                 SSB_108 
                 SSB 
                 Bacterial- 
                   Ahrensia  sp R2A130 
                 MAGSVNKVILVGNLGRDPEIRRTQDGKAIANFSIATSETWRDR 
                 342 
               
               
                   
                   
                 SSB 
                   
                 NSGERREKTEWHRIACFNEGLNKVIEQYVKKGSKVYIEGQLQT 
                   
               
               
                   
                   
                   
                   
                 RKWTDNAGVEKYTTEIVLQNFTGVLTMLDSRNSGGDGGGDSF 
                   
               
               
                   
                   
                   
                   
                 GGGGGGGQIGGSGGGNYGGGGSGGGNQGGGFPSGGDMDDDI 
                   
               
               
                   
                   
                   
                   
                 PF 
                   
               
               
                   
               
               
                 SSB_109 
                 SSB 
                 Bacterial- 
                 
                   Parasutterella 
                 
                 MASVNKVIILGNLGRDPETRFSGNNLQITSMSVATTSYRRSAET 
                 343 
               
               
                   
                   
                 SSB 
                 
                   excrementihominis 
                 
                 QERVEETEWHRVVLFGRQAEIAQQYLKKGSRVYLEGRLRTRK 
                   
               
               
                   
                   
                   
                 CAG:233 
                 WEKDGQTHYSTEILADTLQLIDRKSDVVGGGQSVAPQSSGDGF 
                   
               
               
                   
                   
                   
                   
                 ESPAAPRRSEFTSGAPAARPAAPAQPAAPVPSAAPTDDFEADEI 
                   
               
               
                   
                   
                   
                   
                 PF 
                   
               
               
                   
               
               
                 SSB_110 
                 SSB 
                 Viral- 
                   Mycobacterium  phage 
                 MASVDIQQVGNLTADPELRFLPSGVAVAQFSVASTPRVKKGDE 
                 344 
               
               
                   
                   
                 SSB 
                 Troll4,  Mycobacterium  phage 
                 WVDGETVFLRCTVWRELAEGAAETLRKGDQVVVLGKLKQRS 
                   
               
               
                   
                   
                   
                 Gumball,  Mycobacterium  phage 
                 FETKEGEKRTVFEVDGEFVGKSVRARKSRDGGYSAAATEEPPF 
                   
               
               
                   
                   
                   
                 Nova,  Mycobacterium  phage 
                   
                   
               
               
                   
                   
                   
                 SirHarley,  Mycobacterium  phage 
                   
                   
               
               
                   
                   
                   
                 Adjutor,  Mycobacterium  phage 
                   
                   
               
               
                   
                   
                   
                 Butterscotch,  Mycobacterium   
                   
                   
               
               
                   
                   
                   
                 phage PLot,  Mycobacterium   
                   
                   
               
               
                   
                   
                   
                 phage PBI1 
                   
                   
               
               
                   
               
               
                 SSB_111 
                 SSB 
                 Bacterial- 
                   Paenibacillus polymyxa  (strain 
                 MLNRVILIGRLTKDPELRYTPSGVAVTQFTLAVDRPFTSQGGER 
                 345 
               
               
                   
                   
                 SSB 
                 E681) 
                 EADFLPIVTWRQLAETCANYLRKGRLTAVEGRVQVRNYENNE 
                   
               
               
                   
                   
                   
                   
                 GKRVYVTEIVADNVRFLESNRDGGNGGGNSGGAAREESPFGG 
                   
               
               
                   
                   
                   
                   
                 GNSNSGRGNNNSRNNQDPFSDDGKPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_112 
                 SSB 
                 Bacterial- 
                   Neisseria lactamica  Y92-1009 
                 MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRN 
                 346 
               
               
                   
                   
                 SSB 
                   
                 GQRVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRK 
                   
               
               
                   
                   
                   
                   
                 YQGKDGIERTAYDIVANEMKMLGGRNENSGGAPYEEGYGQSQ 
                   
               
               
                   
                   
                   
                   
                 EAYQRPAQQNRQPAPDAPSHPQEAPAAPRRQPVPAAAPVEDID 
                   
               
               
                   
                   
                   
                   
                 DDIPF 
                   
               
               
                   
               
               
                 SSB_113 
                 SSB 
                 Bacterial- 
                   Hafnia alvei  ATCC 51873 
                 MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSETWR 
                 347 
               
               
                   
                   
                 SSB 
                   
                 DKQTGEQKEKTEWHRVVLFGKLAEVAGEYLRKGSQVYIEGAL 
                   
               
               
                   
                   
                   
                   
                 QTRKWTDQAGVEKYTTEIVVNVGGTMQMLGGRQGGGAPMG 
                   
               
               
                   
                   
                   
                   
                 GGQAQGQQGGWGQPQQPQGNQFSGGSQPAARPQSAPAAQPQ 
                   
               
               
                   
                   
                   
                   
                 SNEPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_114 
                 SSB 
                 Bacterial- 
                 
                   Thermaerobacter marianensis 
                 
                 MLNVVVLIGRLVRDPELRYTPSGVAVGGFTLAVDRPFTNQQGE 
                 348 
               
               
                   
                   
                 SSB 
                 (strain ATCC 700841/DSM 
                 READFIDIVVWRKLAETCANHLSKGRLVAVRGRLQVRSYETQ 
                   
               
               
                   
                   
                   
                 12885/JCM10246/7p75a) 
                 DGQRRRVAEVVADDVRFLDRGPGADRGAAPGSPAGDELADFG 
                   
               
               
                   
                   
                   
                   
                 DVTGLPDDDIPF 
                   
               
               
                   
               
               
                 SSB_115 
                 SSB 
                 Bacterial- 
                   Bacillus  sp 2_A_57_CT2 
                 MMNRVVLVGRLTKDPDLRYTPNGVPVASFTLAVNRTFTNQQG 
                 349 
               
               
                   
                   
                 SSB 
                   
                 EREADFINCVVWRKPAENVANFLKKGSLAGVDGRIQSRSYEGQ 
                   
               
               
                   
                   
                   
                   
                 DGKRVYVTEVQAESVQFLEPKNSSGGQGGNPNYGGPRDQDFP 
                   
               
               
                   
                   
                   
                   
                 FGNNSNQNQRQDNRNQGGYTRVDQDPFANDGQIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_116 
                 SSB 
                 Bacterial- 
                 
                   Bartonella schoenbuchensis 
                 
                 MAGSLNKVILIGNLGADPEIRRLNSGDQVANLRIATSESWRDR 
                 350 
               
               
                   
                   
                 SSB 
                 (strain DSM 13525/NCTC 
                 NTNERKERTEWHSIVIFNENLVKIAEQYLKKGNKIYIEGQLQTR 
                   
               
               
                   
                   
                   
                 13165/R1) 
                 KWQDQNGNDRYTTEIVLQKYRGELQMLEGRGAMDGGERMQ 
                   
               
               
                   
                   
                   
                   
                 DTSPLGGGDFGDSSFDRKEDFSQNSNYLEGNFSHQLDDDVPF 
                   
               
               
                   
               
               
                 SSB_117 
                 SSB 
                 Viral- 
                   Streptococcus  phage 
                 MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGE 
                 351 
               
               
                   
                   
                 SSB 
                 V22,  Streptococcus pneumoniae   
                 READFINFQAWRGTADVIAQVCSKGSLIGLTGRLQVRSYEKDG 
                   
               
               
                   
                   
                   
                   
                 QRRYVTEVIAESVALLESRNSQHGQGNSFQNGNSSPFTDPNPFD 
                   
               
               
                   
                   
                   
                   
                 LPNDGLPF 
                   
               
               
                   
               
               
                 SSB_118 
                 SSB 
                 Bacterial- 
                   Anaerococcus hydrogenalis  ACS- 
                 MNKVFLIGRLTKDPDLRYTQQGQAVVSFSLAVDRGLSKQKRQ 
                 352 
               
               
                   
                   
                 SSB 
                 025-V-Sch4 
                 EMESMNRPTADFPRITVWGVQAENVSRYLKKGNQCAIDGRIQT 
                   
               
               
                   
                   
                   
                   
                 GSYQDKDGKMVYTTDVVADRVEFLESRSEGQYQNNNNPMNQ 
                   
               
               
                   
                   
                   
                   
                 DNGFGDMNQDRSYNNSNNFQQNNNQNMNSNNDDFFDDDFTE 
                   
               
               
                   
                   
                   
                   
                 IEDDGRIPF 
                   
               
               
                   
               
               
                 SSB_119 
                 SSB 
                 Bacterial- 
                   Capnocytophaga  sp oral taxon 
                 MNIVGRLTENAQVQTLSNGKQVVNFSVAVNDNYRNKAGENV 
                 353 
               
               
                   
                   
                 SSB 
                 338 str F0234 
                 QQTSFFDCAYWLSTGVAPYLTKGTLVELEGRVSARAWLNREG 
                   
               
               
                   
                   
                   
                   
                 EPQAGLNFHTSKIKFHFSKKAEVTPNTSASEANNANAITPLPKP 
                   
               
               
                   
                   
                   
                   
                 QVTTGQVAEEDEDDLPF 
                   
               
               
                   
               
               
                 SSB_120 
                 SSB 
                 Viral- 
                 Enterobacteria phage 
                 MASKGVNKVILVGNLGQDPEVRYMPNGGAVANLSLATSDTW 
                 354 
               
               
                   
                   
                 SSB 
                 HK629,  Salmonella  phage HK620 
                 TDKQTGDKKERTEWHRVVLYGKLAEIASEYLRKGSQVYIEGA 
                   
               
               
                   
                   
                   
                 (Bacteriophage HK620) 
                 LRTRKWTDQSGVEKYTTEVVVSQSGTMQMLGGRSNAGNGQQ 
                   
               
               
                   
                   
                   
                   
                 QGGWGQPQQPAAPSHSGTPPQQHPASEPPMDFDDDIPFAPFGH 
                   
               
               
                   
                   
                   
                   
                 SVSRHALYALS 
                   
               
               
                   
               
               
                 SSB_121 
                 SSB 
                 Bacterial- 
                 
                   Methyloversatilis universalis 
                 
                 MASVNKVIIVGNLGRDPETRYAPNGDAICNITVATTDSWKDKQ 
                 355 
               
               
                   
                   
                 SSB 
                 (strain ATCC BAA-1314/JCM 
                 TGERKEQTEWHRVSFYGRLAEIAGQYLRKGSPVYVEGSLRTRK 
                   
               
               
                   
                   
                   
                 13912/FAM5) 
                 WQDKEGQDRYTTEIRAEQMQMLGSRQGAGGEGGGSGGGYGG 
                   
               
               
                   
                   
                   
                   
                 GGGGYGGGDDFDQAPPQRQSAPRQAPSRPQSAPSAPPASSGGG 
                   
               
               
                   
                   
                   
                   
                 FGGMDDDIPF 
                   
               
               
                   
               
               
                 SSB_122 
                 SSB 
                 Bacterial- 
                   Streptococcus infantis  SK970 
                 MINNVVLVGRMVRDAELRYTPSNVAVATFTLAVNRTFKSQNG 
                 356 
               
               
                   
                   
                 SSB 
                   
                 EREADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDN 
                   
               
               
                   
                   
                   
                   
                 QQGQRVYVTEVVAENFQMLESRSVREGQTGGAHSAPSSNYSA 
                   
               
               
                   
                   
                   
                   
                 PTQSVPDFSRDENPFGTTNPLDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_123 
                 SSB 
                 Viral- 
                   Listonella  phage phiHSIC 
                 MASRGVNKVILVGNLGNDPEIRYMPGGAAVANITIATSDSWRD 
                 357 
               
               
                   
                   
                 SSB 
                   
                 KATGEQREKTEWHRVALFGKLAEVAGEYLRKGSQVYIEGQLQ 
                   
               
               
                   
                   
                   
                   
                 TRKWQDQSGQDRYTTEVVVQGFNGVMQMLGGRAQGGAPAQ 
                   
               
               
                   
                   
                   
                   
                 GGMPQQQQQGGGWGQPQQPAMQKQPQQQQSAPQQAQPQYN 
                   
               
               
                   
                   
                   
                   
                 EPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_124 
                 SSB 
                 Bacterial- 
                   Lactobacillus ruminis  SPM0211 
                 MINRVVLVGRLTRDPDLRYTNSGTSVASFTVAVDRNFTNQQG 
                 358 
               
               
                   
                   
                 SSB 
                   
                 NREADFINCVVWGKSAENFANFTHKGSLVGIEGRIQTRSYENQ 
                   
               
               
                   
                   
                   
                   
                 QGNRVYVTEVVTENFSLLESKAESDRYRAQHGGSASSAPRQQS 
                   
               
               
                   
                   
                   
                   
                 QSSFVGNPYGAPANNQGSYQQDNGYGNVNNDAMQDPFAGNG 
                   
               
               
                   
                   
                   
                   
                 SKTDVSEDDLPF 
                   
               
               
                   
               
               
                 SSB_125 
                 SSB 
                 Bacterial- 
                 
                   Paenibacillus mucilaginosus 
                 
                 MLNRVILIGRLTRDPELRYTPAGVAVTQFTLAVDRPFSSNQGQR 
                 359 
               
               
                   
                   
                 SSB 
                 3016 
                 EADFIPVVTWRQLAETCANYLRKGRLAAVEGRIQVRNYDNNE 
                   
               
               
                   
                   
                   
                   
                 GRRVYVTEVIADNVRFLESPNSGNREDGSGMGGSSGGGSSSGG 
                   
               
               
                   
                   
                   
                   
                 GNRGSYGGGREQQDPFQDDGRPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_126 
                 SSB 
                 Bacterial- 
                   Simkania negevensis  (strain 
                 MNQLTIMGHLGADPEVRFTSSGQKVTTLRVAENQKRGGKDET 
                 360 
               
               
                   
                   
                 SSB 
                 ATCC VR-1471/Z) 
                 LWWRITIWGDQFDKLVSYLKKGSAIIVTGEMSKPEIYNDRDGK 
                   
               
               
                   
                   
                   
                   
                 PQISLNMTAYHIAFSPFGRTEKQPQEEPAMAGQSSGMSGFGGD 
                   
               
               
                   
                   
                   
                   
                 QGQQHHYHKGGYDQSHMSQGQGPTSYNEPSDDEIPF 
                   
               
               
                   
               
               
                 SSB_127 
                 SSB 
                 Bacterial- 
                   Sporosarcina newyorkensis  2681 
                 MINRVVLVGRLTKDPELKYTQSGIAVTRFTLAVNRAFSNQQGE 
                 361 
               
               
                   
                   
                 SSB 
                   
                 READFINCVAWRKQAENIANYLRKGSLAGVDGRIQTGSFEGQD 
                   
               
               
                   
                   
                   
                   
                 GKRVYTTEVVADSTQFLEPRSANQERPQTPSYGGAPSYNNAPS 
                   
               
               
                   
                   
                   
                   
                 QDQGYNQQSYQPNQQNMTRVDNDPFQPGGGPIEVTDDDLPF 
                   
               
               
                   
               
               
                 SSB_128 
                 SSB 
                 Bacterial- 
                 
                   Lactobacillus reuteri 
                 
                 MLNRAVLTGRLTRDPELRYTTSGTAVVSFTLAVDRQFRNQNG 
                 362 
               
               
                   
                   
                 SSB 
                   
                 DRDADFINCVIWRKSAENFSNFTHKGSLVGIEGRIQTRNYENQQ 
                   
               
               
                   
                   
                   
                   
                 GNRVYVTEVVVDNFALLEPRQNGGMNQSGMQQPFNNNQQSF 
                   
               
               
                   
                   
                   
                   
                 GAQAPQYGSQPQPGNNAPQSNPSPSMDNGFDPNQNAGNQFPG 
                   
               
               
                   
                   
                   
                   
                 SSDDGGQSIDLADDELPF 
                   
               
               
                   
               
               
                 SSB_129 
                 SSB 
                 Bacterial- 
                   Bacillus subtilis  subsp 
                 MLNRVVLVGRLTKDPELRYTPNGAAVATFTLAVNRTFTNQSG 
                 363 
               
               
                   
                   
                 SSB 
                   spizizenii  (strain TU-B-10), 
                 EREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTRNYEN 
                   
               
               
                   
                   
                   
                   Jeotgalibacillus   marinus   
                 QQGQRVFVTEVQAESVQFLEPKNGGGSGSGGYNEGNSGGGQY 
                   
               
               
                   
                   
                   
                   
                 FGGGQNDNPFGGNQNNQKRNQGNSFNDDPFANDGKPIDISDD 
                   
               
               
                   
                   
                   
                   
                 DLPF 
                   
               
               
                   
               
               
                 SSB_130 
                 SSB 
                 Bacterial- 
                   Thiorhodovibrio  sp 970 
                 MASRGVNKVILIGNLGNDPEIRYFPNGDAVTNLSIATSESWKDR 
                 364 
               
               
                   
                   
                 SSB 
                   
                 NTGEPQERTEWHRIVIRGKLAEIAKQYLRKGSKVFIEGKLRTRK 
                   
               
               
                   
                   
                   
                   
                 WQGQDGQDRYTTEVIVDMTGSMQMLDSRPSGGDYGADNSGG 
                   
               
               
                   
                   
                   
                   
                 SWTSDPAPGIGTAAATTYAQAPYPDQQSPQQQAPAPQNSGQYP 
                   
               
               
                   
                   
                   
                   
                 SQYPNQQPAQSPAPPPDQSGPGLDEPFDDDIPF 
                   
               
               
                   
               
               
                 SSB_131 
                 SSB 
                 Bacterial- 
                   Paenibacillus lactis  154 
                 MLNRVILIGRLTRDPELRYTPSGVAVTQFTLAVDRPFTGQGGER 
                 365 
               
               
                   
                   
                 SSB 
                   
                 EADFIPVVTWRQLAETCANYLRKGRLTAVEGRIQVRNYENNE 
                   
               
               
                   
                   
                   
                   
                 GKRVYVTEVIADNVRFLESNREGGGGGNREESSFGGGSGSGNR 
                   
               
               
                   
                   
                   
                   
                 GGNNFSRNNQDPFSDEGKPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_132 
                 SSB 
                 Viral- 
                   Streptococcus  phage M102 
                 MINNVVLVGRTTKEIELKYTSNNLAYANFTLAVNRNFKNQNG 
                 366 
               
               
                   
                   
                 SSB 
                   
                 EREADFINIVIWRQQAENLANWAKKGTLLGITGRIQTRNYENQ 
                   
               
               
                   
                   
                   
                   
                 QGQRVYVTEVVADNFQILERREVQANTQKPAQQEAFSDVDDI 
                   
               
               
                   
                   
                   
                   
                 DLPF 
                   
               
               
                   
               
               
                 SSB_133 
                 SSB 
                 Bacterial- 
                   Commensalibacter intestini  A911 
                 MAGSVNKVILVGNLGKDPDVRTTQMGTKVVNLTLATSDTWN 
                 367 
               
               
                   
                   
                 SSB 
                   
                 DRQTGERKENTEWHRVVIFNERLADVAEKYLRKGRKVFIEGQ 
                   
               
               
                   
                   
                   
                   
                 LKTRKWTDQQGMERYTTEVVVDRFRGELVLLDSNRSGGDDM 
                   
               
               
                   
                   
                   
                   
                 GYGDDYGASAPMAPASRPAMSAPSAPSKSAGGGWDSSMPGH 
                   
               
               
                   
                   
                   
                   
                 NDLDDEIPF 
                   
               
               
                   
               
               
                 SSB_134 
                 SSB 
                 Bacterial- 
                 
                   Desulfitobacterium 
                 
                 MLNRVVLIGRLTKDPELRYTPSGVAVATFTLAVDRNEKNSNGE 
                 368 
               
               
                   
                   
                 SSB 
                   metallireducens  DSM 15288 
                 RDTDFIPCVVYRQLAELCANFLSKGRLASVDGRLQVRSFEGQD 
                   
               
               
                   
                   
                   
                   
                 GQRRWVTEVIAENVQFLSPKENGNSGNGTSGNASNVGTYGHE 
                   
               
               
                   
                   
                   
                   
                 VSLDDDIPF 
                   
               
               
                   
               
               
                 SSB_135 
                 SSB 
                 Bacterial- 
                   Paenibacillus terrae  (strain 
                 MLNRVILIGRLTKDPELRYTPSGVAVTQFTLAVDRPFTSQGGER 
                 369 
               
               
                   
                   
                 SSB 
                 HPL-003) 
                 EADFLPIVTWRQLAETCANYLRKGRLTAVEGRVQVRNYENNE 
                   
               
               
                   
                   
                   
                   
                 GKRVYVTEIVADNVRFLESNRDGGNGGGNSGGAAREESPFGG 
                   
               
               
                   
                   
                   
                   
                 GNSNSGRGNNNSRNNHDPFSDDGKPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_136 
                 SSB 
                 Bacterial- 
                   Bradyrhizobium  sp STM 3843 
                 MAGSVNKVILVGNLGKDPEIRRTQDGRPIANLSIATSETWRDK 
                 370 
               
               
                   
                   
                 SSB 
                   
                 ATGERKEKTEWHRVVIFNEGLCKVAEQYEKKGAKVYIEGALQ 
                   
               
               
                   
                   
                   
                   
                 TRKWTDQSGVEKYSTEVVLQGFNSTLTMLDGRGGGGGSFADE 
                   
               
               
                   
                   
                   
                   
                 PGGDFGSSGPSMAPPRRAVASAGGGRNSDMDDDIPF 
                   
               
               
                   
               
               
                 SSB_137 
                 SSB 
                 Bacterial- 
                   Frateuria aurantia  (strain ATCC 
                 MARGINKVILVGNLGGDPEERYTGGGTAVCQLRVATAETWND 
                 371 
               
               
                   
                   
                 SSB 
                 33424/DSM 6220/NBRC 3245/ 
                 KQSGQRQERTEWHRVVLFGKEGEIAQEYLRKGRQVYIEGSLRT 
                   
               
               
                   
                   
                   
                 NCIMB13370) ( Acetobacter   
                 KEYTDKEGIKRFTTEVIATDMQMLSGDGGSSGNRQQPGNSRGR 
                   
               
               
                   
                   
                   
                   aurantius ) 
                 GQQANQRGHAQQHEPPPDQGAPPFDDDDIPF 
                   
               
               
                   
               
               
                 SSB_138 
                 SSB 
                 Bacterial- 
                   Bacillus  sp 1NLA3E 
                 MMNRVVLVGRLTKDPDLRYTPNGVAVATFTLAVNRSFSNQQG 
                 372 
               
               
                   
                   
                 SSB 
                   
                 EREADFINCVVWRRPAENVANFLKKGSLAGVDGHIQTRHYEG 
                   
               
               
                   
                   
                   
                   
                 QDGKRVYVTEVVAESVQFLEPKSSASGDRGGSGTYNEPREQQ 
                   
               
               
                   
                   
                   
                   
                 GSPFGNSNNNQNQNQRQNNNNKGYTRVDEDPFAGDGQIDISD 
                   
               
               
                   
                   
                   
                   
                 DDLPF 
                   
               
               
                   
               
               
                 SSB_139 
                 SSB 
                 Bacterial- 
                 
                   Paenibacillus dendritiformis 
                 
                 MLNRVILIGRLTRDPELRYTPSGVAVTQFTLAVDRPFSNQSGER 
                 373 
               
               
                   
                   
                 SSB 
                 C454 
                 EADFIPVVTWRQLAETCANYLRKGRLTAVEGRIQVRNYDNNE 
                   
               
               
                   
                   
                   
                   
                 GKRVYVTEVIADNVRFLESNRDSGGSRDDMGGGYGGGQPNN 
                   
               
               
                   
                   
                   
                   
                 NSRPYGGGGSNQSRGPAADPFSDDGRPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_140 
                 SSB 
                 Bacterial- 
                 gamma proteobacterium BDW918 
                 MARGINKVILVGNLGQDPETRYMPSGGAVTNVTIATSETWKD 
                 374 
               
               
                   
                   
                 SSB 
                   
                 KQSGQPQERTEWHRVVFFNRLAEIAGEYLKKGSKVYVEGSLRT 
                   
               
               
                   
                   
                   
                   
                 RKWQNKEGVDQYTTEIVAAEMQMLDGRGGAGGGASGGASN 
                   
               
               
                   
                   
                   
                   
                 YDDGGYGQQQAPQAQAAAPAPRRAPPPQQNRAPAAPAQNPPA 
                   
               
               
                   
                   
                   
                   
                 GFDDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_141 
                 SSB 
                 Bacterial- 
                 
                   Haemophilus 
                 
                 MAGVNKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDK 
                 375 
               
               
                   
                   
                 SSB 
                   paraphrohaemolyticus  HK411 
                 NTGERREATEWHRIVFYRRQAEVAGQYLRKGSQVYVEGRLKT 
                   
               
               
                   
                   
                   
                   
                 RKWQDQNGQDRYTTEIQGDVLQMLGSRNQGGEMGGQGGWS 
                   
               
               
                   
                   
                   
                   
                 QSNNQGGNWNQAPASNNYNQGNSYNNNYSQTASKPVAKPAQ 
                   
               
               
                   
                   
                   
                   
                 AEPPMDNFDDDIPF 
                   
               
               
                   
               
               
                 SSB_142 
                 SSB 
                 Viral- 
                   Xanthomonas  phage OP2 
                 MASRGVNKVILVGNLGNDPEIRYMPNGGAVANITVATSESWN 
                 376 
               
               
                   
                   
                 SSB 
                   
                 DKQTGEKKEVTEWHRVVLFGKVAEIAGEYLKKGSQVYIEGQL 
                   
               
               
                   
                   
                   
                   
                 KTRKWEKDGVERYTTEIVVNVGGTMQMIGKAPEGGSGGGNRS 
                   
               
               
                   
                   
                   
                   
                 ASNTGWGQPQQPQHSGTPNNKPQNRPSANHQGAAQNGEPPM 
                   
               
               
                   
                   
                   
                   
                 NFDDDIPF 
                   
               
               
                   
               
               
                 SSB_143 
                 SSB 
                 Bacterial- 
                   Nitrolancea hollandica  Lb 
                 MARRDLNKIQIIGNLGRDPEMRYTPGGTPVTEFSVAVNRPPRR 
                 377 
               
               
                   
                   
                 SSB 
                   
                 GQDGQATEETDWFRVVCWDKLAEITDQYLKKGSRVYIEGRLQ 
                   
               
               
                   
                   
                   
                   
                 IRKYTGNDGVDRTSVEIIARDMLMLSGREEGGYAGREEGGTRR 
                   
               
               
                   
                   
                   
                   
                 EPASSRSGDSGEEDFDDLPF 
                   
               
               
                   
               
               
                 SSB_144 
                 SSB 
                 Bacterial- 
                   Herbaspirillum  sp YR522 
                 MASVNKVIIVGNLGRDPETRYMPNGEAVTNIAVATTESWKDK 
                 378 
               
               
                   
                   
                 SSB 
                   
                 NSGEKKELTEWHRITFYRKLAEIAGQYLKKGSQIYVEGRLQTR 
                   
               
               
                   
                   
                   
                   
                 KWTDKDGGERYTTEIIADTMQMLGSRQGGGGGGGSMDDGGS 
                   
               
               
                   
                   
                   
                   
                 YGGGGGGYGGGAPRQAGGGAGGGGGASAPRQQPARQPASNN 
                   
               
               
                   
                   
                   
                   
                 FQDMDDDIPF 
                   
               
               
                   
               
               
                 SSB_145 
                 SSB 
                 Bacterial- 
                   Rhizobium  sp CF080 
                 MAGSVNKVILVGNVGADPEIRRTQDGRPIANLRIATSETWRDR 
                 379 
               
               
                   
                   
                 SSB 
                   
                 NNGERREKTEWHTVVVFNEGLCKVVEQYVKKGAKLYIEGAL 
                   
               
               
                   
                   
                   
                   
                 QTRKWQDQTGNDRYSTEIVLQGFNSTLTMLDGRGEGGGDRGG 
                   
               
               
                   
                   
                   
                   
                 AGGNRVGNDFGGNDFGGGDDYERRPAAGGASRGGQSSGGQP 
                   
               
               
                   
                   
                   
                   
                 AGGNFSRDLDDDIPF 
                   
               
               
                   
               
               
                 SSB_146 
                 SSB 
                 Bacterial- 
                   Paenibacillus alvei  DSM 29 
                 MLNRVILIGRLTRDPELRYTSSGVAVTQFTLAVDRPFSSQGGER 
                 380 
               
               
                   
                   
                 SSB 
                   
                 EADFIPVVTWRQLAETCANYLRKGRLTAVEGRIQVRNYDNNE 
                   
               
               
                   
                   
                   
                   
                 GKRVYVTEIIADNVRFLESNRDNGGTRDDMGSNYGAPAPQYN 
                   
               
               
                   
                   
                   
                   
                 APARGGNSNSRGQAAADPFSDDGRPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_147 
                 SSB 
                 Viral- 
                   Lactobacillus  phage 
                 MINRVVLAGRPTRNLELKSIKSGNSVCSFTLAVDRNFKSKSGER 
                 381 
               
               
                   
                   
                 SSB 
                 KC5a,  Lactobacillus  phage 
                 EADFINCVAWGKTAEVMSQYVKKGSAIGVDGRIQTRSYDNRD 
                   
               
               
                   
                   
                   
                 phi jlb1 
                 GQRVYVTEVVVENFSFLSDPPKNSQVSKNNQSLNQSNDPFDSN 
                   
               
               
                   
                   
                   
                   
                 GQVDIADDDLPF 
                   
               
               
                   
               
               
                 SSB_148 
                 SSB 
                 Bacterial- 
                   Gordonia soli  NBRC 108243 
                 MAGDTVITVIGNLTADPELRFTPSGAAVANFTVASTPRTFDRQT 
                 382 
               
               
                   
                   
                 SSB 
                   
                 NEWKDGEALFLRCNIWRDAAENVTESLTKGSRVIVQGRLKQR 
                   
               
               
                   
                   
                   
                   
                 SFETREGEKRTVVELEVDEIGPSLRYATAKVNKASRGGGGGGG 
                   
               
               
                   
                   
                   
                   
                 FGSSGGGSRGGGSNQQVADDPWGSAPASSGSFGGGDDEPPF 
                   
               
               
                   
               
               
                 SSB_149 
                 SSB 
                 Bacterial- 
                 
                   Streptomyces rimosus 
                 
                 MSFGETPVTIIGNLTADPELKYTTGGQALARFTVASTPRTFDRE 
                 383 
               
               
                   
                   
                 SSB 
                   
                 ANQWKDGTSTFFRCATWRALAEHVAESLTKGSRVVLSGRIRQ 
                   
               
               
                   
                   
                   
                   
                 HNWQTEQGENRSMLAVEVDEIGASLRFTTVTIEGKRTNGTAPA 
                   
               
               
                   
                   
                   
                   
                 DDPWNTAGNPAKTDEEPPF 
                   
               
               
                   
               
               
                 SSB_150 
                 SSB 
                 Bacterial- 
                 
                   Paeniclostridium sordellii 
                 
                 MNHVVLVGRLTKDPELRYIPGTGTAVATFTIAIDRDYAKKDGS 
                 384 
               
               
                   
                   
                 SSB 
                 ( Clostridium sordellii ) 
                 RETDFIPVEVMGKSAEFCANYISKGREVALQGSIRVDNYQNQS 
                   
               
               
                   
                   
                   
                   
                 GERRTFTKVSARNIQALDSNKNRSDSPYPSQNQSFEPSFEPSFEP 
                   
               
               
                   
                   
                   
                   
                 TGLDPQGFQAIDDDDIPF 
                   
               
               
                   
               
               
                 SSB_151 
                 SSB 
                 Viral- 
                 
                   Pseudomonas 
                 
                 MATPVFWEGNIGSAPEHRSFPNGNNPPRQLLRLNVMFDNSIPD 
                 385 
               
               
                   
                   
                 SSB 
                   aeruginosa  ,  Pseudomonas   
                 GQGGYKDRGGFWCSVEWWHQDAQRFAELFAKGMRVKVEGR 
                   
               
               
                   
                   
                   
                   aeruginosa  DHS01,  Pseudomonas   
                 AIMDRWPDKESGEEVQALKVEASRISILPHRLAEVTLLPSTNGQ 
                   
               
               
                   
                   
                   
                 phage LKA5,  Pseudomonas  phage 
                 ATQHQQTRQVSQQDYDSAFDDDIPM 
                   
               
               
                   
                   
                   
                 F116 
                   
                   
               
               
                   
               
               
                 SSB_152 
                 SSB- 
                 Bacterial- 
                   Lactobacillus rossiae  DSM 
                 MINRAVLTGRLTRDPEVRYTQSGAAVGSFTLAVDRQFTNQQG 
                 386 
               
               
                   
                   
                 SSB 
                 15814 
                 QREADFINCVIWRKSAENFANFTHKGSLVGIEGRIQTRNYENQQ 
                   
               
               
                   
                   
                   
                   
                 GQRVYVTEVVVENFALLESRSQSDQRTSGNTNDNGGYNNNAP 
                   
               
               
                   
                   
                   
                   
                 QSGSANPFGGTGNNGGNNAGNTAPSSQSSQTPADPFAGNGESI 
                   
               
               
                   
                   
                   
                   
                 DISDDDLPF 
                   
               
               
                   
               
               
                 SSB_153 
                 SSB 
                 Bacterial- 
                 
                   Nocardia terpenica 
                 
                 MFGAITPTVIGNLTADPELFFSKKGEAGVHFTVACNDRYYDKD 
                 387 
               
               
                   
                   
                 SSB 
                   
                 AERYKDLPAVFMRCTAWGALAEHISDSLSRGMTVIAYGRLKQ 
                   
               
               
                   
                   
                   
                   
                 SSYTVKDTGEKRTVMEMTIDVLGPSLQYATAVVTKASRAATVI 
                   
               
               
                   
                   
                   
                   
                 GEPIDDPWGDAAEQTPVSVGAGEQSSPEGDDDKPPF 
                   
               
               
                   
               
               
                 SSB_154 
                 SSB 
                 Bacterial- 
                   Spiroplasma kunkelii  CR2-3x 
                 MNQFTAIGRTTKDIEIKKTNNGKEYAIFQLAVARPHSNKKETDF 
                 388 
               
               
                   
                   
                 SSB 
                   
                 IPCQVWNKQASVLQQYCQKGSQIAIKGILQSFKDKDNKIHWMV 
                   
               
               
                   
                   
                   
                   
                 RVYSYEFLQTKNNLNNDTYNDIQSITPNITDQTQLIPRNIRLEEV 
                   
               
               
                   
                   
                   
                   
                 ETKELFENQDDTILWD 
                   
               
               
                   
               
               
                 SSB_155 
                 SSB 
                 Bacterial- 
                   Enterococcus faecalis  (strain 
                 MINNLTLVGRLTKDVDLRYTKSGTAVGQFILAVNRNFTNQNG 
                 389 
               
               
                   
                   
                 SSB 
                 ATCC 700802/V583) 
                 DREADFINCVIWRKAAESLANYARKGTLIGLTGRIQTRNYENQ 
                   
               
               
                   
                   
                   
                   
                 QGQRVYVTEVVIENFQLLESKEVNEQRRGQSTGAGQATFDKQP 
                   
               
               
                   
                   
                   
                   
                 TDKPDPLDPFEQGNSPIEISDDDLPF 
                   
               
               
                   
               
               
                 SSB_156 
                 SSB 
                 Viral- 
                   Mycobacterium  phage Hamulus, 
                 MALTLPVVTIEGTLTADPTLNFTQGGTAVSNITIACNTRRKNPQ 
                 390 
               
               
                   
                   
                 SSB 
                   Mycobacterium  phage Dante, 
                 TDQWEDGDTTFMRGTIWKQLAENVSDSLRKGDRVLAHGVLK 
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Ardmore, 
                 QKDYEKDGVKRTAYELDIEGIGPSLRFATASVTKSSGGSGGGA 
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Llij, 
                 RQQPKEDPWGGSSDDWGGDF 
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Drago, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Phatniss, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Spartacus, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Boomer, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage SiSi, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage PMC, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Ovechkin, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Ramsey, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage Fruitloop, 
                   
                   
               
               
                   
                   
                   
                   Mycobacterium  phage SG4 
                   
                   
               
               
                   
               
               
                 SSB_157 
                 SSB 
                 Bacterial- 
                   Dialister  sp CAG:486 
                 MNSVQVMGNLARDPVVRATKTGRAMASFTVAVNRSFTTPQG 
                 391 
               
               
                   
                   
                 SSB 
                   
                 EQREITDWINVVAWGNLAEAVGNQLKKGSRVFVEGRFTTRSY 
                   
               
               
                   
                   
                   
                   
                 DTPDGQRRWVSEVTANFVALPIGGFSHSQQQPSGNAFGGNNG 
                   
               
               
                   
                   
                   
                   
                 YTGNNGYGGNNGGFNNGASPFGQSQPQGQPKSSFGQFGQASK 
                   
               
               
                   
                   
                   
                   
                 DEDIPF 
                   
               
               
                   
               
               
                 SSB_158 
                 SSB 
                 Bacterial- 
                   Faecalibacterium  sp CAG:82 
                 MLNVVAIMGRLVADPELRTTQSGINVVSFRIACDRNFARQGEQ 
                 392 
               
               
                   
                   
                 SSB 
                   
                 RQADFIDIVAWRQQAEFVSKYFQKGSLIAIEGSLQTRQYQDKN 
                   
               
               
                   
                   
                   
                   
                 GNNRTAVEVVANNISFAGPKSNNQGGGSYQNAAPSYQNQAPA 
                   
               
               
                   
                   
                   
                   
                 RPAAVEAAPSYSSGSADDFAVIDDSDDLPF 
                   
               
               
                   
               
               
                 SSB_159 
                 SSB 
                 Bacterial- 
                   Clostridium  sp CAG:470 
                 MNKVILMGRLTRDPEVRYTQTNNTLVASFSLAVNRRFARQGE 
                 393 
               
               
                   
                   
                 SSB 
                   
                 ERQADFINIVAWSKLGEFCSKYFKKGQQVGIIGRIQTRTWDDD 
                   
               
               
                   
                   
                   
                   
                 QGVKHYVTEVVAEEAYFADSKREGDMGAGTFENTFGNTMPG 
                   
               
               
                   
                   
                   
                   
                 SMGSDFETSSTDDLPF 
                   
               
               
                   
               
               
                 SSB_160 
                 SSB 
                 Bacterial- 
                   Prevotella  sp CAG:873 
                 MNKVMLIGNVGKDPDVKYYDADQAVAQFSLATTERGYQLPN 
                 394 
               
               
                   
                   
                 SSB 
                   
                 GTRVPDRTDWHNIVMWGNLAKIVERYVHKGDRLYVEGKMRY 
                   
               
               
                   
                   
                   
                   
                 RYYDDKKGQRRFIAEVYADNMELLTPRSAANTAADSTSQNTN 
                   
               
               
                   
                   
                   
                   
                 AAANNANQNAASSSNASIASTEDDDQLPF 
                   
               
               
                   
               
               
                 SSB_161 
                 SSB 
                 Viral- 
                   Pseudomonas  phage vB_Pae- 
                 MARGVNKVILVGTCGQDPEVRYLPNGNAVTNLSLATSEQWTD 
                 395 
               
               
                   
                   
                 SSB 
                 Kakheti25 
                 KQSGQKVERTEWHRVSLFGKVAEIAGEYLRKGSQVYIEGKLQT 
                   
               
               
                   
                   
                   
                   
                 REWEKDGIKRYTTEIIVDMQGTMQLLGGRPQNQQGGGDQYNQ 
                   
               
               
                   
                   
                   
                   
                 GGGNNYNQGGQQQQYNQAPQRQQAPRPQQQRPAPQQPAPQP 
                   
               
               
                   
                   
                   
                   
                 AADFDSFDDDIPF 
                   
               
               
                   
               
               
                 SSB_162 
                 SSB 
                 Bacterial- 
                 
                   Avibacterium paragallinarum 
                 
                 MAGVNKVIIVGNLGNDPEIRTMPNGEAVANISVATSESWMDK 
                 396 
               
               
                   
                   
                 SSB 
                 JF4211 
                 NTGERREITEWHRIVFYRRQAEVAGEYERKGSKVYVEGRLRTR 
                   
               
               
                   
                   
                   
                   
                 KWQDQNGQDRYTTEIQGDVLQMLDSRADRGQGGYSAQGGYA 
                   
               
               
                   
                   
                   
                   
                 QQGSNQYAQPAQPSYQAPQQQAAARPSSPPTPMVDDNFDDDN 
                   
               
               
                   
                   
                   
                   
                 IPF 
                   
               
               
                   
               
               
                 SSB_163 
                 SSB 
                 Bacterial- 
                   Streptomyces  sp HPH0547 
                 MAMGDTPVTVVGNLVADPELKYTQSGTALARFTVASTPRSYD 
                 397 
               
               
                   
                   
                 SSB 
                   
                 RESGQYKDGTAMFMRCSAWRGLAENIASSLAKGNRVVVTGRL 
                   
               
               
                   
                   
                   
                   
                 RQHNWQTPEGENRSMLALEVDEIGPSLRFATAQPAKADNETK 
                   
               
               
                   
                   
                   
                   
                 KTAPPADDPWNTTPAPAGGDEPPF 
                   
               
               
                   
               
               
                 SSB_164 
                 SSB 
                 Bacterial- 
                   Dermabacter  sp HFH0086 
                 MANDTVITVVGNLTADPELRFTNSGIPVASFTVASTPRTFDRQS 
                 398 
               
               
                   
                   
                 SSB 
                   
                 GEWKDGEALFLRCSIWRDAAENVAESLTKGTRVIVQGRLQQR 
                   
               
               
                   
                   
                   
                   
                 SYTDREGNNRTSIELQVDEIGPSLRYATAKPTRTQRGGGGNFG 
                   
               
               
                   
                   
                   
                   
                 GGFNGGNSGGGNYGGGQGGYSNQGGYGGNRGGAQGGPQGG 
                   
               
               
                   
                   
                   
                   
                 QNPADVDPWSNGGAEEPPPF 
                   
               
               
                   
               
               
                 SSB_165 
                 SSB 
                 Bacterial- 
                   Treponema socranskii  subsp 
                 MTDINHVLVIGRLTRDFGADPRTFFYTTGGTACAKVSIAVNRS 
                 399 
               
               
                   
                   
                 SSB 
                   socranskii  VPI DR56BR1116 = 
                 VKQSDGQWTDEVSFFDVTIWGKTAENLKPYLVKGKQIAVDGY 
                   
               
               
                   
                   
                   
                 ATCC 35536 
                 LKQDRWQKDGQNFSKVNIVANSVQLLGGGTSAPESAPAPQNY 
                   
               
               
                   
                   
                   
                   
                 GRVQETYRDNPSQVPPRMQGNYMQPQQPAYQTTAPQQQFGG 
                   
               
               
                   
                   
                   
                   
                 GDDFPEDLPF 
                   
               
               
                   
               
               
                 SSB_166 
                 SSB 
                 Bacterial- 
                 
                   Lactobacillus shenzhenensis 
                 
                 MINRVTLVGRLTQDVEVKHTESGIAVANFTVAVERHFKNAEGE 
                 400 
               
               
                   
                   
                 SSB 
                 LY-73 
                 KQADFVTCKMWRKSAENFANFTCKGSLVGILGEVRTHTYEKD 
                   
               
               
                   
                   
                   
                   
                 GQKVYRTDIEADTFALLEPKAVTEARRAGTLKSGSGGGSDNVF 
                   
               
               
                   
                   
                   
                   
                 AAAGANGEKIDITDDDLPF 
                   
               
               
                   
               
               
                 SSB_167 
                 SSB 
                 Bacterial- 
                 
                   Bifidobacterium magnum 
                 
                 MIQVTFTGNAGQDPETKTFNNGGSITQVNVGIGQGYKDRASGQ 
                 401 
               
               
                   
                   
                 SSB 
                   
                 WIDKGTAWVTVKANTSQTKETLQHVHKGTHLLVTGSLTVRTY 
                   
               
               
                   
                   
                   
                   
                 QKQDGTQGTALDVNATAIAIIPRKQQQMQQPQQPMQQPVQQP 
                   
               
               
                   
                   
                   
                   
                 QQQNTWASMPTGTDPWSQGSFNQEPEF 
                   
               
               
                   
               
               
                 SSB_168 
                 SSB 
                 Bacterial- 
                   Borrelia duttonii  CR2A 
                 MISKEWKSGVGFMMMGVLMSDINNITLSGRLVKDSLLSYSSTN 
                 402 
               
               
                   
                   
                 SSB 
                   
                 LAILNFSIANNIKVKREGEWEDNAQFFNCVLFGKRAETLFHFLS 
                   
               
               
                   
                   
                   
                   
                 KGKQVVVNGSMRHEYYKNKHSEVNKIKSIIFVEQLRLFGADSK 
                   
               
               
                   
                   
                   
                   
                 HHNPKVDIPIPVPPPVPDSACEFNEDIPF 
                   
               
               
                   
               
               
                 SSB_169 
                 SSB 
                 Viral- 
                   Pseudomonas  phage 
                 MRGVNKVILVGNVGGDPETRYMPNGNAVTNITLATSESWKDK 
                 403 
               
               
                   
                   
                 SSB 
                 vB_PaeP_C1-14_Or,  Pseudomonas   
                 QTGQQQERTEWHRVVFFGKLAEIVGQHVKKGQQLYVEGSLRT 
                   
               
               
                   
                   
                   
                 phage vB_PaeP_p2- 
                 RKWQAQDGQDRYTTEIIVDMHGQMQMFGGKPGNEQAAQSRS 
                   
               
               
                   
                   
                   
                 10_Or1,  Pseudomonas  phage PaP3 
                 STQQQSAPQQRSAQDEFDDDIPL 
                   
               
               
                   
               
               
                 SSB_170 
                 SSB 
                 Bacterial- 
                 
                   Mycobacterium brisbanense 
                 
                 MAGDTTITVIGNLTADPELRFTPSGAAVANFTVASTPRTFDRQT 
                 404 
               
               
                   
                   
                 SSB 
                   
                 NEWKDGEALFLRCNIWREAAENVAESLTRGSRVIVQGRLKQRS 
                   
               
               
                   
                   
                   
                   
                 FETREGEKRTVVELEVDEIGPSLRYATAKVNKASRSGGGGGGF 
                   
               
               
                   
                   
                   
                   
                 GGGGGGFSGGGGGSRQSEPKDDPWGSAPASGSFSGADDEPPF 
                   
               
               
                   
               
               
                 SSB_171 
                 SSB 
                 Bacterial- 
                 
                   Pseudoalteromonas lipolytica 
                 
                 MARGVNKVILVGNLGQDPEVRYMPNGNGVANISIATTDSWKD 
                 405 
               
               
                   
                   
                 SSB 
                 SCSIO 04301 
                 KNTGQMQERTEWHRVVLFGKLAEVAGEYLRKGSQVYIEGRLQ 
                   
               
               
                   
                   
                   
                   
                 TRKWTDQSGQEKFTTEIVVDMGGQMQMLGGRGGDQQGGGY 
                   
               
               
                   
                   
                   
                   
                 QGGQSQGGYQGGQQQGGGYGGGSQQAQSNSYAPQQQSAPAP 
                   
               
               
                   
                   
                   
                   
                 AQQQRPQQQPAPAPAPQQNNNQYGGYGQQQSSAPQQGGFAP 
                   
               
               
                   
                   
                   
                   
                 KPQNAPQGGASNPMEPPIDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_172 
                 SSB 
                 Bacterial- 
                   Candidatus Accumulibacter  sp 
                 MASVNKVILVGNLGADPETRYLPSGDAVCNIRMATTDRSRDK 
                 406 
               
               
                   
                   
                 SSB 
                 SK-12 
                 VSGELKEYTEWHRVVFFGKLAETAGQYLKKGRQVYVEGRIRT 
                   
               
               
                   
                   
                   
                   
                 NKWQDKEGNERYTTEIVANEMKMLGSREGMGAPAGEAEYGG 
                   
               
               
                   
                   
                   
                   
                 SMPSAAGQAAGAARNAPARKTPGFEDMDDDIPF 
                   
               
               
                   
               
               
                 SSB_173 
                 SSB 
                 Viral- 
                   Listeria  phage B054,  Listeria   
                 MMNRVVLVGRLTKDPELRYTPAGVAVATFTLAVNRPFKNAQ 
                 407 
               
               
                   
                   
                 SSB 
                 
                   monocytogenes 
                 
                 GEQEADFINCVVWRKPAENAANFLKKGSMAGVDGRVQTRNY 
                   
               
               
                   
                   
                   
                   
                 EDSDGKRVFVTEVVAETVQFLEPKNINAEGATSNNYQNQANY 
                   
               
               
                   
                   
                   
                   
                 SNNNKTSSYRADTSQKSDSFADEGKAIDINEDDLPF 
                   
               
               
                   
               
               
                 SSB_174 
                 SSB 
                 Bacterial- 
                 
                   Hydrogenovibrio marinus 
                 
                 MRGVNKVILVGTLGRDPEMKYAANGNAIANLSLATSENWTDK 
                 408 
               
               
                   
                   
                 SSB 
                   
                 NSGQKQEKTEWHRVVIFGKLAEIAGQYLTKGSQIYLEGKLQTR 
                   
               
               
                   
                   
                   
                   
                 KWQDQNTGQDRYSTEVVIDFNGQMQMLGGGNKPQGQGAAP 
                   
               
               
                   
                   
                   
                   
                 QGQGFAGQQPPQGQPMANQQQQAPQQNQQPQAGNQAAMQN 
                   
               
               
                   
                   
                   
                   
                 QAPPMQQAPAYPENDFGDDDVPF 
                   
               
               
                   
               
               
                 SSB_175 
                 SSB 
                 Viral- 
                   Lactobacillus  phage phig1e 
                 MSINSVVLTGRLTKDVDLRATNSGKNVARFTLAVDRNFKSEQ 
                 409 
               
               
                   
                   
                 SSB 
                   
                 QADFFTVSVWGKQAENTAKYCHKGSLVGVRGHLRSGSYDKN 
                   
               
               
                   
                   
                   
                   
                 GQKVYFVDIEADSVQFLDTRNNSQDAPQSENLGAQSDFGFQSG 
                   
               
               
                   
                   
                   
                   
                 QTYHSGADNSQSTFNSFGGTSQNDYPF 
                   
               
               
                   
               
               
                 SSB_176 
                 SSB 
                 Bacterial- 
                   Pedobacter antarcticus , 
                 MSGINKVILVGHLGKDPEVRHLDGGVTVASFPLATSETYNKEG 
                 410 
               
               
                   
                   
                 SSB 
                   Pedobacter   antarcticus  4BY 
                 KRIEQTEWHNIVLWRGLAEVASKYLQKGKEVYIEGKERTRSFE 
                   
               
               
                   
                   
                   
                   
                 DKERVKKYVTEVVAENFTLLGRKSDFENTPVAAATPKHENDY 
                   
               
               
                   
                   
                   
                   
                 VEPEDNATGDLPF 
                   
               
               
                   
               
               
                 SSB_177 
                 SSB 
                 Bacterial- 
                 
                   Salinisphaera hydrothermalis 
                 
                 MASKGVNRAIILGNEGADPEVRHTAGGTAVANISVATSEVWT 
                 411 
               
               
                   
                   
                 SSB 
                 C41B8 
                 DKNSGEKQERTEWHRVVAFARLAEVMGEFLKSGSKVYIEGKI 
                   
               
               
                   
                   
                   
                   
                 QTRKWQNREGQDVYTTEIVANELQMLDSKPQGNAGAQSRAN 
                   
               
               
                   
                   
                   
                   
                 NQAQNYGAASRGGAPARGGQQRQSPPQYQPPAGGDPGFDDDL 
                   
               
               
                   
                   
                   
                   
                 DDDIPL 
                   
               
               
                   
               
               
                 SSB_178 
                 SSB 
                 Bacterial- 
                   Endozoicomonas montiporae , 
                 MARGVNKVILIGNLGGDPDVRFTPNGSAVANFNVATSESWTD 
                 412 
               
               
                   
                   
                 SSB 
                   Endozoicomonas montiporae  CL- 
                 KNTGQRQDRTEWHRVVVFGKLAEICQQYLRKGSKVYLEGKLQ 
                   
               
               
                   
                   
                   
                 33 
                 TRKWQDQQGQDRYTTEVVVDGFGGQMQMLDSRQDGGMGAV 
                   
               
               
                   
                   
                   
                   
                 PNQMGGGYQQPQAAPQQQAPQQQAPQQRAPQQQYAAPQQQA 
                   
               
               
                   
                   
                   
                   
                 QPQAQQRPAPAQQQAAPQPAAAGFDDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_179 
                 SSB 
                 Bacterial- 
                 
                   Nitratireductor basaltis 
                 
                 MAGSVNKVILVGNLGADPEIRRLNSGDPVVNLRIATSETWRDR 
                 413 
               
               
                   
                   
                 SSB 
                   
                 GTGERRERTEWHNVVIFNDNLAKVAEQYLKKGAKVYLEGQL 
                   
               
               
                   
                   
                   
                   
                 QTRKWQDQQGQDRYTTEVVLQKFRGELQMLDTRGQGGDNQI 
                   
               
               
                   
                   
                   
                   
                 GYGGGGGGGQRSDFGQSSPAEPSGGGGGGGYSRDLDDEIPF 
                   
               
               
                   
               
               
                 SSB_180 
                 SSB 
                 Bacterial- 
                   Paenibacillus  sp FSL R7-0331 
                 MLNRIILIGRLTRDPELRYTPAGVAVTQFTLAVDRNFTGQNGER 
                 414 
               
               
                   
                   
                 SSB 
                   
                 EADFIPVVTWRQLAETCANYLRKGRLAAVEGRIQVRNYENNE 
                   
               
               
                   
                   
                   
                   
                 GKRVYVTEVIADNVRFLESSQNREGGNAPSGGSMPEEPAFGGG 
                   
               
               
                   
                   
                   
                   
                 NGGNSARGNNNNFSRSNNNQDPFSGDGKPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_181 
                 SSB 
                 Bacterial- 
                 
                   Burkholderia cenocepacia 
                 
                 MASVNKVILVGNLGADPEVRYLPSGDAVANIRLATTDRYKDK 
                 415 
               
               
                   
                   
                 SSB 
                 (strain ATCC BAA-245/DSM 
                 ASGDFKEMTEWHRVAFFGRLAEIVSEYLKKGSSVYIEGRIRTRK 
                   
               
               
                   
                   
                   
                 16553/LMG 16656/NCTC 13227/ 
                 WQGQDGQDRYSTEIVADQMQMLGGRGGSGGGGGGGDEGGY 
                   
               
               
                   
                   
                   
                 J2315/CF5610) ( Burkholderia   
                 GGGYGGGGGRGGEQMERGGGGGRAGGAARGGAGGGQSRPS 
                   
               
               
                   
                   
                   
                   cepacia  (strain 
                 APAGGGFDEMDDDIPF 
                   
               
               
                   
                   
                   
                 J2315)),  Burkholderia   
                   
                   
               
               
                   
                   
                   
                   cenocepacia  BC7 
                   
                   
               
               
                   
               
               
                 SSB_182 
                 SSB 
                 Bacterial- 
                   Bifidobacterium reuteri  DSM 
                 MGVNVSFTGNVGRDPETREFDNGRSLTTFSVGVSQGYYDQQN 
                 416 
               
               
                   
                   
                 SSB_23975 
                   
                 QWHDQGTMWITVECSPTAARQLPYVHKGVKLLVTGRLSQRFY 
                   
               
               
                   
                   
                   
                   
                 QKKDGGQGSELRVYADAIGFLHRKDEQPQTGGFTGAQPQPPAS 
                   
               
               
                   
                   
                   
                   
                 DPWAAPQSDTEPEF 
                   
               
               
                   
               
               
                 SSB_183 
                 SSB 
                 Viral- 
                   Lactococcus  phage 
                 MAIITVTAQANEKNTRTVNTAKGDKKIISVPLFEKEKGSNVKV 
                 417 
               
               
                   
                   
                 SSB 
                 phi311,  Lactococcus  phage 
                 AYGSAELPDFIQLGDTVTISGRVQAKESGEYVNYNFVFPTIEKV 
                   
               
               
                   
                   
                   
                 ul36k1t1,  Lactococcus  phage 
                 FISNDNGKQAQAKQDLFGGSEPIEVNTEDLPF 
                   
               
               
                   
                   
                   
                 ul362,  Lactococcus  phage 
                   
                   
               
               
                   
                   
                   
                 ul361,  Lactococcus   lactis , 
                   
                   
               
               
                   
                   
                   
                   Lactococcus  phage ul36k1 
                   
                   
               
               
                   
               
               
                 SSB_184 
                 SSB 
                 Bacterial- 
                   Helicobacter  sp MIT 05-5294 
                 MFNKVILVGNLTRDVELRYLPSGSALAKLGLAVNRRFKKQDG 
                 418 
               
               
                   
                   
                 SSB 
                   
                 SQGDEVCYIDVNLFGRTAEVANQYLKRGSSVLIEGRLVLESWT 
                   
               
               
                   
                   
                   
                   
                 DNNGQKRSKHSITAETMQMLGSRNEGGNYAGNGGGNGNYGN 
                   
               
               
                   
                   
                   
                   
                 SYSNDSYNDMHNGGYNNYGSYQNTQPSAPKPQKPKENDIPEID 
                   
               
               
                   
                   
                   
                   
                 IDDEEIPF 
                   
               
               
                   
               
               
                 SSB_185 
                 SSB 
                 Viral- 
                   Prochlorococcus  phage P-SSM2 
                 MNHCLLEVTVKVAPTIRYTQDNQTAIAEMDVEFDGFRADDPP 
                 419 
               
               
                   
                   
                 SSB 
                   
                 GSIKVVGWGNLAQDLQSHVQIGQRLIIEGRLRMNTVPRQDGSK 
                   
               
               
                   
                   
                   
                   
                 EKRAEFTLSKIHSSTPKGTISPNKTSPNQVPSNDSPSLNALTSKEP 
                   
               
               
                   
                   
                   
                   
                 ENPKSDNDSVTWNSSPLIPDTDDIPF 
                   
               
               
                   
               
               
                 SSB_186 
                 SSB 
                 Viral- 
                   Flavobacterium  phage 1 lb 
                 MNRQEFIGHIGNDAEVKDLGINQVINDSVAVSESYVNKTTNEKI 
                 420 
               
               
                   
                   
                 SSB 
                   
                 TNTTWYECAKWGNNTQIAQYLKKGQQVYIMGKPNNRAWQN 
                   
               
               
                   
                   
                   
                   
                 EQGDIKVVNAVKVTEILLLGGKQSNDNNAQPQQPQQQPQQPQ 
                   
               
               
                   
                   
                   
                   
                 QAPQPQNNEDFNNSEEHDDLPF 
                   
               
               
                   
               
               
                 SSB_187 
                 SSB 
                 Bacterial- 
                   Paenibacillus  sp P1XP2 
                 MLNRVILIGRLTKDPELRYTPAGVAVTQFTLAVDRPFTSQGGER 
                 421 
               
               
                   
                   
                 SSB 
                   
                 EADFIPVVTWRQLAETCANYLRKGRLTAVEGRIQVRNYENNE 
                   
               
               
                   
                   
                   
                   
                 GRRVYVTEVIADNVRFLESNREGGSGGGQGPREESSFGGGSRE 
                   
               
               
                   
                   
                   
                   
                 NNNYSRSNSNQDPFFDDGKPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_188 
                 SSB 
                 Bacterial- 
                 
                   Mameliella alba 
                 
                 MAGSVNKVILVGNEGRDPETRTFQNGGKVCNLRIATSENWKD 
                 422 
               
               
                   
                   
                 SSB 
                   
                 RNTGERRERTEWHSVAIFSEPLARIAEQYLRKGSKVYIEGQLET 
                   
               
               
                   
                   
                   
                   
                 RKWQDQNGQDRYSTEVVLRPYRGELTLLDSRSEGGGGGGFGG 
                   
               
               
                   
                   
                   
                   
                 GSGGGYGGGGGGYDDRGGYDDPGYGGGSGGGSGGGSGPSRA 
                   
               
               
                   
                   
                   
                   
                 PARDIDDEIPF 
                   
               
               
                   
               
               
                 SSB_189 
                 SSB 
                 Bacterial- 
                   Sulfurovum  sp ES06-10 
                 MNQFTGLGNLTRDIELRYLQNGSAIATCGLAMNRRFKKQDGT 
                 423 
               
               
                   
                   
                 SSB 
                   
                 QGEEVCFIDITFFGRTAEIANQYLSRGRKVLIIGRLKLDQWTDQ 
                   
               
               
                   
                   
                   
                   
                 QGVKRSKHSIHVESLEMIDSQNSEAQSTHPQPQAHNNQTQRQP 
                   
               
               
                   
                   
                   
                   
                 QNTPRQPQSNPGQYSGHDIPDIDINDDEIPF 
                   
               
               
                   
               
               
                 SSB_190 
                 SSB 
                 Bacterial- 
                 
                   Streptomyces cyaneogriseus 
                 
                 MNETMICAVGNVATHPVYRELASGPSARFRLAVTSRYWDREK 
                 424 
               
               
                   
                   
                 SSB 
                   
                 SAWTDGHTNFFTVWAHRQLAANAAASLAVGDPVMVQGRLK 
                   
               
               
                   
                   
                   
                   
                 VRTDVREGQSRTSADIDAVAIGHDETRGTSAFRRTNKPDSASTS 
                   
               
               
                   
                   
                   
                   
                 PRPEPNWETPAAESGDPSEQQPTGEPALM 
                   
               
               
                   
               
               
                 SSB_191 
                 SSB 
                 Viral- 
                   Thalassomonas  phage BA3 
                 MAGVNKVIILGNLGKDPEVRFMPNGGGVANLTIATSETWKDK 
                 425 
               
               
                   
                   
                 SSB 
                   
                 QTGEQKEKTEWHRVVMFGKLAEIAGEYLKKGSKVYIEGQLQT 
                   
               
               
                   
                   
                   
                   
                 RKWQNQQGQDQYTTEIVVQGFNGTMQMLDSRGQGGGGQGG 
                   
               
               
                   
                   
                   
                   
                 GFQGQQQGGFSGGQQAPQQQGGFQQQAPKQQGGFSQQAAPQ 
                   
               
               
                   
                   
                   
                   
                 QQGGFAQQAPQQQAPQQGGFSGGQQAPQQGGFQQQQGGFGQ 
                   
               
               
                   
                   
                   
                   
                 QNQQQAAKVNPQEPSIDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_192 
                 SSB 
                 Bacterial- 
                   Clostridium  sp FS41 
                 MNSAQLVGRLTRDPEVRYSDGGTTVARFTLAVDRRFKKDGGD 
                 426 
               
               
                   
                   
                 SSB 
                   
                 DADFINCVAFGKTAEFLEKWFRKGQRLGLTGRIQTGSYVNQEG 
                   
               
               
                   
                   
                   
                   
                 TKVYTTEVVVENVEFVESKGASAGDGGPQQRPAPTSAIGDGFM 
                   
               
               
                   
                   
                   
                   
                 NIPDGVEDDGLPFN 
                   
               
               
                   
               
               
                 SSB_193 
                 SSB 
                 Bacterial- 
                   Acetobacter orientalis  21F-2 
                 MAGSVNKVILVGNLGKDPEVRTTQGGQKIVSFSLATSDTWND 
                 427 
               
               
                   
                   
                 SSB 
                   
                 RQSGERRERTEWHRVVVFNEREADVAERFLRKGRKVYLEGAL 
                   
               
               
                   
                   
                   
                   
                 QTRKWTDQSGQERFTTEVIVERFRGELVLLDSRADNEGGQSNQ 
                   
               
               
                   
                   
                   
                   
                 QPQPREQPRQQGGYGQQSNGWGGSSDEDDSIPF 
                   
               
               
                   
               
               
                 SSB_194 
                 SSB 
                 Bacterial- 
                 
                   Microbacterium ginsengisoli 
                 
                 MAGETVITVVGNLTADPELRYTQNGLPVANFTIASTPRTFDRQ 
                 428 
               
               
                   
                   
                 SSB 
                   
                 ANEWKDGEALFLRASVWREFAEHVAGSLTKGSRVIATGRLRQ 
                   
               
               
                   
                   
                   
                   
                 RSYQDRDGNNRTAIELEVDEIGPSLRYATAQVTRAAGGSGAGG 
                   
               
               
                   
                   
                   
                   
                 GGSRAQVADEPWSTPGSSNSSADAWSAPGAYGDDTPF 
                   
               
               
                   
               
               
                 SSB_195 
                 SSB 
                 Viral- 
                   Staphylococcus  phage 
                 MNTVNLIGNLVADPELKGQNNNVVNFVIAVQRQEKNKQTNEY 
                 429 
               
               
                   
                   
                 SSB 
                 SA97,  Staphylococcus  phage 
                 ETDFIRCVAFGKTAEIIANNFNKGNKIGVTGSIQTGSYENNQGQ 
                   
               
               
                   
                   
                   
                 phi7247PVL,  Staphylococcus   
                 KVFTTDIAVNNITFVERKNTQAKHGLLTRN 
                   
               
               
                   
                   
                   
                 phage phiETA3,  Staphylococcus   
                   
                   
               
               
                   
                   
                   
                   aureus ,  Staphylococcus  phage 
                   
                   
               
               
                   
                   
                   
                 phi5967PVL 
                   
                   
               
               
                   
               
               
                 SSB_196 
                 SSB 
                 Bacterial- 
                 
                   Salinicoccus halodurans 
                 
                 MLNRVVLVGRLTKDPDLKVSQNNISVATFTLAVNRPFTSSNGE 
                 430 
               
               
                   
                   
                 SSB 
                   
                 RGADFINCVVFRKQAENVNQYLKKGSLAGVDGRLQSRTYDNK 
                   
               
               
                   
                   
                   
                   
                 DGQRVFVTEVVCDSVQFLEPKGQGGNQQQQYNNNSADSYTN 
                   
               
               
                   
                   
                   
                   
                 AYGNQSTGSRPAPQQQSRQDNAEENPFANADGPVDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_197 
                 SSB 
                 Viral- 
                   Lactobacillus  phage phiadh 
                 MINRVVLVGRLTRDPDELRYTNSGTSVASFTVAVDRNFTNQQG 
                 431 
               
               
                   
                   
                 SSB 
                   
                 NREADFINCVVWGKSAENFANFTHKGSLVGIEGRIQTRSYENQ 
                   
               
               
                   
                   
                   
                   
                 QGNRVYVTEVVTENFSLLESKAESDRYRAQHGGSASSAPRQQS 
                   
               
               
                   
                   
                   
                   
                 QSSFGGNPYGAPANNQGSYQQDNAYGNGNNDAMQDPFAGNG 
                   
               
               
                   
                   
                   
                   
                 SKTDISEDDLPF 
                   
               
               
                   
               
               
                 SSB_198 
                 SSB 
                 Bacterial- 
                 Microgenomates group bacterium 
                 MSSRSLNKVQIIGNLTRDPELRYTPQGTAVCQIGVATNRSWTN 
                 432 
               
               
                   
                   
                 SSB 
                 GW2011_GWF1_44_10 
                 DAGEKNEETEFHKVVAWSKLAEICSQLLKKGRKIYLEGRLQTR 
                   
               
               
                   
                   
                   
                   
                 DWTTQDGQKRQTTEIVMDNMILLDSAGRGGDGEGASTSYTND 
                   
               
               
                   
                   
                   
                   
                 DTSSKPVAKKSKKADVADDASSAEEAPVAEDVSDDIPF 
                   
               
               
                   
               
               
                 SSB_199 
                 SSB 
                 Bacterial- 
                 Parcubacteria group bacterium 
                 MNLNKAFVLGNLTRDPELRTTTTGQSVAQFGVATNREFTDKS 
                 433 
               
               
                   
                   
                 SSB 
                 GW2011_GWA2_42_14 
                 GQRQKLTEFHNIVAWGKLGELCHQYIKKGQSVFVEGRIQTRSW 
                   
               
               
                   
                   
                   
                   
                 DDKQTGQKKYRTEIVAENVQFGPKPFRNETAGQNQAQPETPKE 
                   
               
               
                   
                   
                   
                   
                 KEEILETVQYPADEEEIKPEEIPF 
                   
               
               
                   
               
               
                 SSB_200 
                 SSB 
                 Bacterial- 
                 
                   Labilithrix luteola 
                 
                 MAEGLNKVMLLGNLGADPELKMTAGGQAVLKLRLATTETYL 
                 434 
               
               
                   
                   
                 SSB 
                   
                 DRNNSRQERTEWHSVTLWGKRGEALAKFLSKGERIFVEGSLRT 
                   
               
               
                   
                   
                   
                   
                 SSYEKDGEKRYRTEINATNVILAGRAGRGAGDEMGGGGGGGG 
                   
               
               
                   
                   
                   
                   
                 GFGGGGGGGGGGGGGGFERRAPSRSGGGGGGGFEGGGRGAP 
                   
               
               
                   
                   
                   
                   
                 ASAPPADDFGDYPGGDDEIPF 
                   
               
               
                   
               
               
                 SSB_201 
                 SSB 
                 Bacterial- 
                   Sphingopyxis  sp (strain 113P3) 
                 MAGSVNKVILIGNLGADPEIKSFQNGGKIANIRIATSESWKDRM 
                 435 
               
               
                   
                   
                 SSB 
                   
                 TGERKERTEWHNVVINGDGLVGVVERYLKKGSKVYIEGSLRT 
                   
               
               
                   
                   
                   
                   
                 RKWQDRDGNDRYTTEVVVAGMGGSLTMLDGAPGGGGSRTSG 
                   
               
               
                   
                   
                   
                   
                 DSWNQGGGSSGGWDQGGSSGGGWNQGGGSSGGGRPPFDDDL 
                   
               
               
                   
                   
                   
                   
                 DDDVPF 
                   
               
               
                   
               
               
                 SSB_202 
                 SSB 
                 Bacterial- 
                   Actinobacteria bacterium  OK074 
                 MSNETIITVVGNLVDDPELRWTSSSVAVAKFRIASTPRTFDKQT 
                 436 
               
               
                   
                   
                 SSB 
                   
                 NEWKDGESLFLTCSVWRQAAEHVAESLQRGMRVIVQGRLKQR 
                   
               
               
                   
                   
                   
                   
                 SYEDREGVKRTVYELDVEELGPSLRNATAKVTKAGGSGQARE 
                   
               
               
                   
                   
                   
                   
                 ALQQARTRSSREGREDPWASSGAAAESAQAGAWGDAPPF 
                   
               
               
                   
               
               
                 SSB_203 
                 SSB 
                 Viral- 
                   Thermus  phage phiYS40 
                 DFIDPLEGRGRETLEDARGQPRLRHALNQVILMGNLTRDPDLR 
                 437 
               
               
                   
                   
                 SSB 
                   
                 YTPQGTAVARLGLAINERRPGQGPDGERTHFIEVQAWRDLAE 
                   
               
               
                   
                   
                   
                   
                 WAEELKRGEGLLVIGRLVNDSWTSSTGERRFQTRVEALRLERP 
                   
               
               
                   
                   
                   
                   
                 TRGPERTGGSRPQEPERSVQTGGVDIDEGLEDFPPEEDLPF 
                   
               
               
                   
               
               
                 SSB_204 
                 SSB 
                 Bacterial- 
                 
                   Lentibacillus amyloliquefaciens 
                 
                 MMNRVVLVGRLTRDPDLRYTPNGVAVANFRIAIDRPDSNQQG 
                 438 
               
               
                   
                   
                 SSB 
                   
                 NRDADFINCVVWRRAAENLATYMKKGSMIGVDGRIQSRSFEG 
                   
               
               
                   
                   
                   
                   
                 RDGNTVFVTEVVADNIQFLESKGTSQSRDQQPSGFQPNQNQNQ 
                   
               
               
                   
                   
                   
                   
                 NQNQTTQTQTNENPFKDNGEPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_205 
                 SSB 
                 Viral- 
                   Phormidium  phage Pf-WMP3 
                 MAEIVQDPQLRYTSDNQTAITELLVQIDPLRDGDPPETLKVVA 
                 439 
               
               
                   
                   
                 SSB 
                   
                 WRRLAEAVQENFHRGDRVVIEGRLGMVVFDRPEGFREKRAEV 
                   
               
               
                   
                   
                   
                   
                 TAQRIHLLDRAAAGSAPPAAPTAAVPSSAPVTPMNGPANTPAN 
                   
               
               
                   
                   
                   
                   
                 APAPVSSPDEPLSDDIPF 
                   
               
               
                   
               
               
                 SSB_206 
                 SSB 
                 Bacterial- 
                   Lactobacillus capillatus  DSM 
                 MINRAILVGRLTRDPDLRYTANGVAVATFTVAVNRQFTNQQG 
                 440 
               
               
                   
                   
                 SSB 
                 19910 
                 EREADFINCVIWRKSAENFANFTHKGSLVGVDGRIQTRSYENQ 
                   
               
               
                   
                   
                   
                   
                 QGNRVYVTEIVVDSFSELESRSQSERYQQQHGADTQGSAPSQN 
                   
               
               
                   
                   
                   
                   
                 SSNPNNDNLFGNSTKNNPPKARENNTDVDPFADSGKQIDISDD 
                   
               
               
                   
                   
                   
                   
                 DLPF 
                   
               
               
                   
               
               
                 SSB_207 
                 SSB 
                 Bacterial- 
                   Candidatus Cloacimonas  sp SDB 
                 MTSELRLPRVNYVVLSGRLTRDVDLRFIPNGTPVAKLSLAFDR 
                 441 
               
               
                   
                   
                 SSB 
                   
                 NYQKDGEWQQETSVIDVVVWSKRGEQCAEYLQKGSPVLIEGY 
                   
               
               
                   
                   
                   
                   
                 IKTRSYQDKDNNNRKVTEIIASKINFLEKKPYSSEDDSETKNDSS 
                   
               
               
                   
                   
                   
                   
                 DTNKSKADIIDDDVPF 
                   
               
               
                   
               
               
                 SSB_208 
                 SSB 
                 Bacterial- 
                 
                   Roseateles depolymerans 
                 
                 MASVNKVIILGNLGRDPELRYTPSGSAVCNVSIATTRNWKSRE 
                 442 
               
               
                   
                   
                 SSB 
                   
                 GGERQEETEWHRVVFYDRLAEIAGEYLKKGRPVYVEGRLKTR 
                   
               
               
                   
                   
                   
                   
                 KWQDKEGKDNYTTEIIAETMQLLGGRDGGDDMGGGGGGGYN 
                   
               
               
                   
                   
                   
                   
                 RERSSGGSRESSGGGSGRDAGDFDSPRAPAPRSAPRPAPAPAAK 
                   
               
               
                   
                   
                   
                   
                 PATGFDDMDDDIPF 
                   
               
               
                   
               
               
                 SSB_209 
                 SSB 
                 Bacterial- 
                 Parcubacteria bacterium 32_520 
                 MNYNRAILCGRVTKAPEILMTPSGHKVAKISLATNEYQGKGKE 
                 443 
               
               
                   
                   
                 SSB 
                   
                 EKTVFHNLIAWDRTADIAQQVIVVGHEIMIEGRIDNRTYKKKD 
                   
               
               
                   
                   
                   
                   
                 GTKGYISEVVIDRLQLGNKPRAVAVPAENTATSNYQEPPADDD 
                   
               
               
                   
                   
                   
                   
                 NVPVIEDMDEIDISSIPF 
                   
               
               
                   
               
               
                 SSB_210 
                 SSB 
                 Bacterial- 
                 
                   Streptomyces longwoodensis 
                 
                 MAGETVITVIGNLVDDPELRFTPSGAAVAKFRVASTPRTFDRQT 
                 444 
               
               
                   
                   
                 SSB 
                   
                 NEWKDGESLFLTCSVWRQAAENVAESLQRGMRVLVQGRLKQ 
                   
               
               
                   
                   
                   
                   
                 RSYEDREGVKRTVYELDVEEVGPSLRNATAKVTKTAGRGGQG 
                   
               
               
                   
                   
                   
                   
                 GGGGFGGGGGGQQGGGWGGGPGGGQQGGGAPADDPWATGA 
                   
               
               
                   
                   
                   
                   
                 PAGGAQQGGGGWGGGSGGGGGYSDEPPF 
                   
               
               
                   
               
               
                 SSB_211 
                 SSB 
                 Bacterial- 
                 
                   Streptomyces albus 
                 
                 MAGETVITVVGNLVDDPELRFTPSGAAVAKFRVASTPRTFDRQ 
                 445 
               
               
                   
                   
                 SSB 
                   
                 TNEWKDGESEFLTCSVWRQAAENVAESLQRGMRVIVQGRLKQ 
                   
               
               
                   
                   
                   
                   
                 RSYEDREGVKRTVFELDVDEVGASLRNATAKVTKTSGRGGQG 
                   
               
               
                   
                   
                   
                   
                 GYGGGGGQGGGGWGGGSGGGQQGGGAPADDPWATGAPSGG 
                   
               
               
                   
                   
                   
                   
                 QQGGGGGGWGGGSGNSGGYSDEPPF 
                   
               
               
                   
               
               
                 SSB_212 
                 SSB 
                 Bacterial- 
                   Pirellula  sp SH-Sr6A 
                 MASYNRVILLGNLVRDIELKYTTSRLAVCQNAIAVNERRKNAA 
                 446 
               
               
                   
                   
                 SSB 
                   
                 GEWVDETSFVDVTFFGRTAEVVAEYLGKGSPIFVEGKLKQDT 
                   
               
               
                   
                   
                   
                   
                 WEKDGQKRSKLYVIVDRMQLIGSRNESKGSGAPRPQSNGNRF 
                   
               
               
                   
                   
                   
                   
                 ADQEQHVSPDMHVSEVGDGGFIDEDMPF 
                   
               
               
                   
               
               
                 SSB_213 
                 SSB 
                 Bacterial- 
                 
                   Bacillus sporothermodurans 
                 
                 MMNRVVLVGRLTKDPDLRYTPSGVAVATFTLAVNRTFTNQQG 
                 447 
               
               
                   
                   
                 SSB 
                   
                 EREADFINCVVWRKPAENVANFLKKGSLAGVDGRLQTRNYEG 
                   
               
               
                   
                   
                   
                   
                 QDGKRVYVTEVVAESVQFLEPRNANANPNRGGNNDFYGGGQ 
                   
               
               
                   
                   
                   
                   
                 GNQNTPFNQNQNQRNQGYTRVDEDPFSNDGQPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_214 
                 SSB 
                 Bacterial- 
                   Akkermansia  sp KLE1798 
                 MANLNKVFLMGNLTADPELRYTPKGTAVTDIRLAINRYYAGD 
                 448 
               
               
                   
                   
                 SSB 
                   
                 NSERQEETTFVDVTLWNRQAEVAGNYLSKGRGVFVEGRLQLD 
                   
               
               
                   
                   
                   
                   
                 SWEDKASGQKRTKLRVIGENIQLFPRGGDSSDMGGAPRQQSAP 
                   
               
               
                   
                   
                   
                   
                 RSNNYGQSQAPQNYNPPPMPSNQQQSNDEGDMDDEIPF 
                   
               
               
                   
               
               
                 SSB_215 
                 SSB 
                 Bacterial- 
                 
                   Coriobacteriales bacterium 
                 
                 MSINRVILSGNLTRTPELRSTANGSSVLGFGIAVNDRRKNPQTG 
                 449 
               
               
                   
                   
                 SSB 
                 DNF00809 
                 EWEDFPNFIDCTVFGPRAEGLSHCLDKGSKISLEGKERWSQWE 
                   
               
               
                   
                   
                   
                   
                 RDGQRRSKLEVIVDEIELMSTRGGQNFAANDHASADAGSYSQP 
                   
               
               
                   
                   
                   
                   
                 YNAPSTPAAPPAVDPSSYNADLPF 
                   
               
               
                   
               
               
                 SSB_216 
                 SSB 
                 Bacterial- 
                 
                   Streptomyces albulus 
                 
                 MAGETVITVVGNLVDDPELRFTPSGAAVAKFRVASTPRTFDRQ 
                 450 
               
               
                   
                   
                 SSB 
                   
                 TNEWKDGESLFLTCSVWRQAAENVAESLQRGMRVVVQGRLK 
                   
               
               
                   
                   
                   
                   
                 QRSYEDREGVKRTVFELDVEEVGPSLKNATAKVTKTTGRGGQ 
                   
               
               
                   
                   
                   
                   
                 GGYGGGQQGGGNWGGAPGGGQQGGGAPADDPWATSAPAGG 
                   
               
               
                   
                   
                   
                   
                 QQQGGGGNWGGSSGGSGGGYSDEPPF 
                   
               
               
                   
               
               
                 SSB_217 
                 SSB 
                 Viral- 
                   Salmonella  phage SETP3 
                 MARGVNKVIIVGTLGNDPEVKYSASGSAIVNISVATSEQWKDK 
                 451 
               
               
                   
                   
                 SSB 
                   
                 QTGEKKEQTEWHRIVIFGKLAEVAGEYLRKGSQVYIEGQLRTR 
                   
               
               
                   
                   
                   
                   
                 KWTDSNGIDRYTTEIVIPQMGGVMQMLGGKRDDSGQQPRQQS 
                   
               
               
                   
                   
                   
                   
                 GQQPQGGWVTNQQQQPQKQQSPQGGNEPPMDESDDIPF 
                   
               
               
                   
               
               
                 SSB_218 
                 SSB 
                 Bacterial- 
                 
                   Streptomyces noursei 
                 
                 MAGETVITVVGNLVDDPELRFTPSGAAVAKFRVASTPRTFDRQ 
                 452 
               
               
                   
                   
                 SSB 
                   
                 TNEWKDGESLFLTCSVWRQAAENVAESLTRGTRVIVQGRLKQ 
                   
               
               
                   
                   
                   
                   
                 RSYEDREGVKRTVYELDVEEVGASLRNATAKVTKTGGRGGQG 
                   
               
               
                   
                   
                   
                   
                 GGGFGGGQGGGQQGGGWGGGPGGGQQGGGAPADDPWATGG 
                   
               
               
                   
                   
                   
                   
                 PSGGGQGGGGGWGGGSGGSGGGYSDEPPF 
                   
               
               
                   
               
               
                 SSB_219 
                 SSB 
                 Viral- 
                   Synechococcus  phage Syn5 
                 MNHCLLEVEVIEAPQLRYTQDNQTPVAEMSVQFEGLRPDDPSG 
                 453 
               
               
                   
                   
                 SSB 
                   
                 QIKVVGWGNLAQDLQNRVQVGQRLMLEGRLRISTITRADGLK 
                   
               
               
                   
                   
                   
                   
                 EKRAEFTLSRLHPLAEGPSPAGDTRPAPLPPAGGQPRSVPRPVP 
                   
               
               
                   
                   
                   
                   
                 ARAAVAGSTTAAAAIPATPAPEVPTWNTSPEVPELSDDDDIPF 
                   
               
               
                   
               
               
                 SSB_220 
                 SSB 
                 Viral- 
                   Streptomyces  phage VWB 
                 MAGETPITVVGNVVADPELRETPSGAPVANFRVASTPRTFDRV 
                 454 
               
               
                   
                   
                 SSB 
                   
                 TNEWKDGDTLFLSVSVWRQQAENVAESIKRGDRVIVVGRLGQ 
                   
               
               
                   
                   
                   
                   
                 RQYEKDGERKSSYEVQAEDVGPALKNATAQVAKNGQQNGQQ 
                   
               
               
                   
                   
                   
                   
                 RAQGYGQGYGQQAPQQGYGAPQADQWSTQQPRQGYTDEPPF 
                   
               
               
                   
               
               
                 SSB_221 
                 SSB 
                 Bacterial- 
                   Lactococcus lactis  subsp 
                 MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGE 
                 455 
               
               
                   
                   
                 SSB 
                   cremoris  (strain MG1363) 
                 READFINCVIWRQQAENLANWAKKGALIGVTGRIQTRNYENQ 
                   
               
               
                   
                   
                   
                   
                 QGQRVYVTEVVADSFQMLESRSARDGMGGGASAGSYSAPSQS 
                   
               
               
                   
                   
                   
                   
                 TNNTPRPQTNNNSATPNFGRDADPFGSSPMEISDDDLPF 
                   
               
               
                   
               
               
                 SSB_222 
                 SSB 
                 Bacterial- 
                   Lactococcus lactis  subsp 
                 MNKTMLIGRLTNAPEISKTTNNKSYVRVTLAVNRRFKNEKGER 
                 456 
               
               
                   
                   
                 SSB 
                   cremoris  (strain MG1363) 
                 EADEISIIIWGKSAETLVSYAKKGSLISVEGEIRTRNYTDKNEQK 
                   
               
               
                   
                   
                   
                   
                 HYITEILGLSYDLLESRATLALRESAVKTEELELEADELPF 
                   
               
               
                   
               
               
                 SSB_223 
                 SSB 
                 Bacterial- 
                   Lactococcus lactis  subsp 
                 MINNVTLVGRITKKPELRYTPQNKAVATFTLAVNRAFKNANGE 
                 457 
               
               
                   
                   
                 SSB 
                   cremoris  (strain MG1363) 
                 READFISCVIWGKSAENLANWTHKGQLIGVIGNIQTRNYENQQ 
                   
               
               
                   
                   
                   
                   
                 GQRVYITEVVASNFQVLEKSNQANGERVSNPAAKPQNNDSFGS 
                   
               
               
                   
                   
                   
                   
                 DPMEISDDDLPF 
                   
               
               
                   
               
               
                 SSB_224 
                 SSB 
                 Bacterial- 
                 
                   Mycobacterium smegmatis 
                 
                 MNMFETPFTVVGNIITNPVRLRFGDQELYKFRVASNSRRRSPEG 
                 458 
               
               
                   
                   
                 SSB 
                   
                 TWEPGNSLYVTVNCWGNLARGVSASLGKGDSVVVVGHLYTN 
                   
               
               
                   
                   
                   
                   
                 EYEDREGVRRSSVEVRATAVGPDLSRCIARVEKVQPSQGPRAD 
                   
               
               
                   
                   
                   
                   
                 AGGDPERVPDDDVDRRDADDEPDDLADDDVADLAGDGLPLT 
                   
               
               
                   
                   
                   
                   
                 A 
                   
               
               
                   
               
               
                 SSB_225 
                 SSB 
                 Bacterial- 
                   Staphylococcus aureus  (strain 
                 MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGE 
                 459 
               
               
                   
                   
                 SSB 
                 Mu50/ATCC 700699) 
                 READFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKE 
                   
               
               
                   
                   
                   
                   
                 GQRVYVTEVIADSIQFLEPKNSNDTQQDLYQQQVQQTRGQSQY 
                   
               
               
                   
                   
                   
                   
                 SNNKPVKDNPFANANGPIELNDDDLPF 
                   
               
               
                   
               
               
                 SSB_226 
                 SSB 
                 Bacterial- 
                   Staphylococcus aureus  (strain 
                 MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGE 
                 460 
               
               
                   
                   
                 SSB 
                 Mu50/ATCC 700699) 
                 READFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQ 
                   
               
               
                   
                   
                   
                   
                 EGRRVFVTEVVCDSVQFLEPKNAQQNGGQRQQNEFQDYGQGF 
                   
               
               
                   
                   
                   
                   
                 GGQQSGQNNSYNNSSNTKQSDNPFANANGPIDISDDDLPF 
                   
               
               
                   
               
               
                 SSB_227 
                 SSB 
                 Bacterial- 
                   Staphylococcus aureus  (strain 
                 MLNRTVLVGRLTKDPELRSTPNGVNVGTFTLAVNRTFTNAQG 
                 461 
               
               
                   
                   
                 SSB 
                 Mu50/ATCC 700699) 
                 EREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTRNYEN 
                   
               
               
                   
                   
                   
                   
                 KDGQRVFVTEVVADSVQFLEPKNNNQQQNNNYQQQRQTQTG 
                   
               
               
                   
                   
                   
                   
                 NNPFDNNADSIEDLPF 
                   
               
               
                   
               
               
                 SSB_228 
                 SSB 
                 Bacterial- 
                   Caulobacter vibrioides  (strain 
                 MAGSVNKVILVGNEGADPEIRSLGSGDRVANLRIATSETWRDR 
                 462 
               
               
                   
                   
                 SSB 
                 ATCC 19089/CB15) 
                 SSGERKEKTEWHRVVIFNDNEVKVAEQYLRKGSTVYIEGALQT 
                   
               
               
                   
                   
                   
                 ( Caulobacter crescentus ) 
                 RKWTDNTGQEKYSTEIVLQKFRGELTMLGGRGGDAGMSSGGG 
                   
               
               
                   
                   
                   
                   
                 DEYGGGYSGGGSSFGGGQRSQPSGPRESFSADLDDEIPF 
                   
               
               
                   
               
               
                 SSB_229 
                 SSB 
                 Bacterial- 
                   Vibrio natriegens  NBRC 15636 = 
                 MASRGINKVILVGNEGNDPEIRYMPNGGAVANITIATSDSWRD 
                 463 
               
               
                   
                   
                 SSB 
                 ATCC 14048 = DSM 759 
                 KATGEQREKTEWHRVVLFGKLAEVAGEYLRKGSQVYVEGQL 
                   
               
               
                   
                   
                   
                   
                 QTRKWQDQSGQDRYSTEVVVQGFNGVMQMLGGRAQGGAPA 
                   
               
               
                   
                   
                   
                   
                 MGGQAPQQGGWGQPQQPAQPQYNAPQQQAPKQSAPQQPQQQ 
                   
               
               
                   
                   
                   
                   
                 YNEPPMDFDDDIPF 
                   
               
               
                   
               
               
                 SSB_230 
                 SSB 
                 Bacterial- 
                   Synechocystis  sp PCC 6803 
                 MSVNSIHLVGRAGRDPEVKYFESGNVVCNFTLAVNRRTSKKD 
                 464 
               
               
                   
                   
                   
                 SSB 
                 EPPDWFDLEIWGKTAEIAGNYVKKGSLIGIQGSLKFDHWEDRN 
                   
               
               
                   
                   
                   
                   
                 SGTPRSKPVIRVNNLDLLGSKRDNAEATMNNYPEEF 
                   
               
               
                   
               
               
                 SSB_231 
                 SSB 
                 Bacterial- 
                   Synechocystis  sp PCC 6803 
                 MNSFVLMATVIREPELRFTKENQTPVCEFLVEFPGMRDDSPKES 
                 465 
               
               
                   
                   
                 SSB 
                   
                 LKVVGWGNLANTIKETYHPGDRLIIEGRLGMNMIERQEGFKEK 
                   
               
               
                   
                   
                   
                   
                 RAELTASRISLVDSGNGINPGELSSPPEPEAVDLSNTDDIPF 
                   
               
               
                   
               
               
                 SSB_232 
                 SSB 
                 Bacterial- 
                   Streptomyces albus  J1074 
                 MAGETVITVVGNLVDDPELRFTPSGAAVAKFRVASTPRTFDRQ 
                 466 
               
               
                   
                   
                 SSB 
                   
                 TNEWKDGESLFLTCSVWRQAAENVAESLQRGMRVIVQGRLKQ 
                   
               
               
                   
                   
                   
                   
                 RSYEDREGIKRTVYELDVDEVGASLRNATAKVTKTTGRGGQG 
                   
               
               
                   
                   
                   
                   
                 GYSGGGGGGGQQGGWGGGPGGGQQQGGGGAPADDPWATSA 
                   
               
               
                   
                   
                   
                   
                 PSGGGQQQGGGGGWGGSSGGGGGYSDEPPF 
                   
               
               
                   
               
               
                 SSB_233 
                 SSB 
                 Bacterial- 
                   Streptomyces albus  J1074 
                 MNETMVTVVGNVATAPVYRESAHGPMARFRMAATPRRWDRE 
                 467 
               
               
                   
                   
                 SSB 
                   
                 RQTWTDGPTSFFTIWTTRQLASNVTASVTVGEPVIVQGRERVR 
                   
               
               
                   
                   
                   
                   
                 ETERGGQQWTTAEIDAASVGHDLTRGTAAFRRVRKPVGTWPG 
                   
               
               
                   
                   
                   
                   
                 GTATEDSRSAAAAQRAAGEPDWSVSGVGAGGDWGVAGGDRS 
                   
               
               
                   
                   
                   
                   
                 GGGGEEPAEAGDRAEASAGTGTGTGTGGGEPEVEAVSPGPGA 
                   
               
               
                   
                   
                   
                   
                 A 
                   
               
               
                   
               
               
                 SSB_234 
                 SSB 
                 Bacterial- 
                   Streptomyces coelicolor  (strain 
                 MNETMICAVGNVATTPVFRDLANGPSVRFRLAVTARYWDREK 
                 468 
               
               
                   
                   
                 SSB 
                 ATCC BAA-471/A3(2)/M145) 
                 NAWTDGHTNFFTVWANRQLATNASGSLAVGDPVVVQGRLKV 
                   
               
               
                   
                   
                   
                   
                 RTDVREGQSRTSADIDAVAIGHDLARGTAAFRRTARTEASTSPP 
                   
               
               
                   
                   
                   
                   
                 RPEPNWEVPAGGTPGEPVPEQRPDPVPVG 
                   
               
               
                   
               
               
                 SSB_235 
                 SSB 
                 Bacterial- 
                   Streptomyces coelicolor  (strain 
                 MAGETVITVVGNLVDDPELRFTPSGAAVAKFRVASTPRTFDRQ 
                 469 
               
               
                   
                   
                 SSB 
                 ATCC BAA-471/A3(2)/M145) 
                 TNEWKDGESLFLTCSVWRQAAENVAESLQRGMRVIVQGRLKQ 
                   
               
               
                   
                   
                   
                   
                 RSYEDREGVKRTVYELDVDEVGASLRSATAKVTKTSGQGRGG 
                   
               
               
                   
                   
                   
                   
                 QGGYGGGGGGQGGGGWGGGPGGGQQGGGAPADDPWATGG 
                   
               
               
                   
                   
                   
                   
                 APAGGQQGGGGQGGGGWGGGSGGGGGYSDEPPF 
                   
               
               
                   
               
               
                 SSB_236 
                 SSB 
                 Bacterial- 
                   Synechococcus  sp UTEX 2973 
                 MNSCILQATVVEAPQLRYTQDNQTPVAEMVVQFPGLSSKDAP 
                 470 
               
               
                   
                   
                 SSB 
                   
                 ARLKVVGWGAVAQELQDRCRLNDEVVLEGRLRINSLLKPDGN 
                   
               
               
                   
                   
                   
                   
                 REKQTELTVTRVHHLTLDSATGILAQEESEVSYGRSAAASAPV 
                   
               
               
                   
                   
                   
                   
                 KASPVVTPTAAPDVDYDDIPF 
                   
               
               
                   
               
               
                 SSB_237 
                 SSB 
                 Bacterial- 
                   Synechococcus  sp UTEX 2973 
                 MSLNVVNLVGRVGRDPEARYFESGSVVCKFSLAVNRRSRNDE 
                 471 
               
               
                   
                   
                 SSB 
                   
                 PDWFNVEMWGREAQVAIDYVKKGSLIGISGALKIESWTDRNN 
                   
               
               
                   
                   
                   
                   
                 NQRTTPVVRANRLELLGSRRDQEGGMAPRDPDSDLF 
                   
               
               
                   
               
               
                 PaSSB 
                 SSB 
                   
                 
                   Pseudomonas aeruginosa 
                 
                 MARGVNKVILVGNVGGDPETRYMPNGNAVTNITLATSESWKD 
                 472 
               
               
                   
                   
                   
                   
                 KQTGQQQERTEWHRVVFF 
                   
               
               
                   
                   
                   
                   
                 GRLAEIAGEYLRKGSQVYVEGSLRTRKWQGQDGQDRYTTEIV 
                   
               
               
                   
                   
                   
                   
                 VDINGNMQLLGGRPSGDD 
                   
               
               
                   
                   
                   
                   
                 SQRAPREPMQRPQQAPQQQSRPAPQQQPAPQPAQDYDSFDDDI 
                   
               
               
                   
                   
                   
                   
                 PF 
                   
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
             
            
               
                   
               
               
                 Minimum inhibitory concentration (MIC) for various 
               
               
                 combinations of ciprofloxacin resistance-conferring alleles. 
               
            
           
           
               
               
               
            
               
                   
                   
                 Ciprofloxacin MIC 
               
               
                   
                 Genotype 
                 (μg/ml) 
               
               
                   
                   
               
            
           
           
               
               
               
            
               
                   
                 PAO1 wild-type 
                 0.25 
               
               
                   
                 nfxB knockout 
                 4 
               
               
                   
                 GyrA_T83I 
                 16 
               
               
                   
                 GyrA_T83I + 
                 32 
               
               
                   
                 ParC_S87L 
                   
               
               
                   
                 GyrA_T83I + 
                 &gt;128 
               
               
                   
                 ParC_S87L + NfxB 
                   
               
               
                   
                 knockout 
               
               
                   
                   
               
            
           
         
       
     
     Materials and Methods 
     Strains and Plasmids 
     A strain of  Escherichia coli , which is derived from MG1655, but which has mutS knocked out, has a mutation in dnaG (Q576A) which decreases its affinity for single-stranded binding protein (SSB) at the replication fork, was used. A plasmid with beta lactamase (carb/amp resistance) on a p15a origin was used. Proteins were cloned by Gibson assembly under the control of the pBAD (arabinose) promoter. 
     The commonly studied strain of  L. lactis , NZ9000, which features a nisin induction system was used. A plasmid with chloramphenicol acetyltransferase (chloramphenicol resistance) was used, built off of pJP005. Proteins were cloned by Gibson assembly under the control of a nisin-inducible promoter. 
       M. smegmatis  strain MC2 155 was studied. A plasmid with a kanamycin resistance gene on a dual origin plasmid (colE1 and oriM) was used. Proteins were cloned by Gibson assembly under the control of a tetracycline-sensitive operator. TetR, the tetracycline operator repressor was also present on the plasmid. 
     Culture, Induction and Transformation 
       E. coli  cultures were grown in standard Lysogeny Broth (LB) at 37° C. in a rotating drum. Overnight cultures were diluted 1:100, grown for 90 minutes, and then single-stranded annealing proteins (SSAPs), or pairs of SSAPs and single-stranded binding proteins (SSBs) were induced with arabinose, grown another 30 minutes, and then prepared for transformation. Briefly, cells were put on ice, washed twice with cold water, and resuspended in 1/100 th  culture volume of water. 
       L. lactis  cultures were grown in M17 media supplemented with 0.5% glucose at 30° C. and not shaken. Overnight cultures were diluted 1:10 into M17 media supplemented with 0.5% w/v glucose, 0.5 M sucrose, and 2.5% w/v glycine. Diluted cultures were grown for three hours and then induced with 5 ng/μl nisin, grown another 30 minutes and then prepared for transformation. Briefly, cells were put on ice, washed twice with a cold buffer containing 0.5 M sucrose and 10% glycerol, resuspended in 1/100 th  culture volume. 
       M. smegmatis  cultures were grown in 7H9 media supplemented with 0.5% w/v BSA, 0.2% w/v glucose, 0.085% w/v NaCl, 0.05% v/v Tween 80, and 0.2% glycerol. Cultures were grown at 37° C. in a rolling drum for two days until confluent, then diluted 1:100 and grown overnight until OD600 reached 0.4-0.8. Cultures were then induced with 400 μg/ml anhydrotetracycline (ATC), put in the incubator for another hour, and then prepared for transformation. Briefly, cells were put on ice, washed twice with cold water, and resuspended in 1/100 th  culture volume. 
     Unless otherwise noted, bacterial cultures were grown in Lysogeny-Broth-Lennox (LB L ) (10 g tryptone, 5 g yeast extract, 5 g NaCl in 1 L H 2 O). Super optimal broth with catabolite repression (SOC) was used for recovery after electroporation. MacConkey agar (17 g pancreatic digest of gelatin, 3 g peptone, 10 g lactose, 1.5 g bile salt, 5 g NaCl, 13.5 g agar, 0.03 g neutral red, 0.001 g crystal violet in 1 L H 2 O) and IPTG-X-gal Mueller-Hinton II agar (3 g beef extract, 17.5 g acid hydrolysate of casein, 1.5 g starch, 13.5 g agar in 1 L H 2 O, supplemented with 40 mg/L X-gal and 0.2 μM IPTG) were used to differentiate LacZ(+) and (−) mutants. Cation-adjusted Mueller Hinton II Broth (MHBII) was used for antimicrobial susceptibility tests. Antibiotics were ordered from Sigma-Aldrich. Recombineering oligos were synthesized by Integrated DNA Technologies (IDT) or by the DNA Synthesis Laboratory of the Biological Research Centre (Szeged, Hungary) with standard desalting as purification. 
     Oligo-Mediated Recombineering 
     Bacterial cultures ( E. coli, K. pneumoniae, C. freundii , or  P. aeruginosa ) were grown in LB L  at 37° C. in a rotating drum. Overnight cultures were diluted 1:100, grown for 60 minutes or until OD600≈0.3, whereupon expression of SSAPs was induced for 30 minutes with 0.2% arabinose or 1 mM m-toluic acid as appropriate. Cells were then prepared for transformation. Briefly,  E. coli, K. pneumoniae , and  C. freundii  cells were put on ice for approximately ten minutes, washed three times with cold water and resuspended in 1/100 th  culture volume of cold water. This same procedure was followed for  P. aeruginosa  with the following differences: (1) Resuspension Buffer (0.5 M sucrose+10% glycerol) was used in place of water and (2) there was no pre-incubation on ice, as competent cell prep was carried out at room temperature, which was found to be much more efficient than preparation at 4° C. After competent cell prep, 9 μl of 100 μM oligo was added to 81 μl of prepared cells for a final oligo concentration of 10 μM in the transformation mixture (2.5 μM final oligo concentration was used for  C. freundii  and  K. pneumoniae ). This mixture was transferred to an electroporation cuvette with a 0.1 cm gap and electroporated immediately on a Gene Pulser (BioRad) with the following settings: 1.8 kV (2.2 kV in the case of  P. aeruginosa ), 200 Ω, 25 Cultures were recovered with SOC media for one hour and then 4 ml of LB with 1.25× selective antibiotic and 1.25× antibiotic for plasmid maintenance were added for outgrowth. 
     Engineering of SEER Chassis 
     The  E. coli  strain described in this work as the SEER chassis is engineered from EcNR2 (Wang et al., Nature 460, 894-898 (2009)). EcNR2 harbors a small piece of λ-phage integrated at the bioAB locus, which allows expression of λ-Red genes, and a knockout of the methyl-directed mismatch repair (MMR) gene mutS, which improves the efficiency of mismatch inheritance (MG1655 ΔmutS::cat Δ(ybhB-bioAB)::[λc1857 Δ(cro-orf206b)::tetR-bla]). Modifications made to EcNR2 to engineer the SEER chassis include: 1. improvement of MAGE efficiency by mutating DNA primase (dnaG_Q576A) (Lajoie et al., Nucleic Acids Res. 40, e170 (2012)), 2. introduction of a handle for SDS selection (tolC_STOP), 3. introduction of a handle for CHL selection (mutS::cat_STOP), and 4. removal of lambda phage with a zeocin resistance marker Δ[λcI857 Δ(cro-orf206b)::tetR-bla]::zeoR, The final strain which was referred to as the SEER chassis is therefore: MG1.655 Δ(ybhB-bioAB)::zeoR ΔmutS::cat_STOP tolC_STOP dnaG_Q576A. 
     Selective Allele Testing in the SEER Chassis 
     To complement the SEER chassis&#39; two engineered selective handles, following native antibiotic resistance alleles were tested: [TMP: FolA P21→L, A26→G, and L28→R], [KAN/GEN: 16SrRNA U1406→A and A1408→G], [SPT: 16SrRNA A1191→G and C1192→U], [RIF: RpoB S512→P and D516→G], [STR: RpsL K4→R and K88→R], and [CIP: GyrA S83→L] (Novais et al., PLoS Pathog. 6, (2010); Criswell et al., Antimicrob. Agents Chemother. 50, 445-452 (2006); Campbell et al., Cell 104, 901-912 (2001); Okamoto-Hosoya et al., Microbiol. Read. Engl. 149, 3299-3309 (2003); Yoshida et al., Antimicrob. Agents Chemother. 34, 1271-1272 (1990)). 90-bp oligos conferring each mutation, with two PT bonds at their 5′ end and with complementarity to the lagging strand were designed. Two oligos were designed to repair the engineered selective handles: (1) elimination of a stop codon in the chloramphenicol acetyltransferase (cat) to confer CHM resistance and (2) elimination of a stop codon in tolC to confer SDS resistance. Oligo-mediated recombineering was run with Redβ expressed off of the pARC8 plasmid and the cultures were then plated onto a range of concentrations of the antibiotic to which the oligo was expected to confer resistance. Colony counts were made and compared to a water-blank control. Modifications targeted to provide TMP, KAN, and SPT resistance did not work adequately and so were dropped. RpsL_K43R was chosen for STR selection and RpoB_S512P for RIF selection, although in both cases there was not a significant observable difference between the two tested alleles. An antibiotic concentration was chosen that provided the largest selective advantage for those cultures transformed with oligo (Fig S2). The concentrations chosen for the selective antibiotics were: 0.1% v/v SDS, 25 μg/ml STR, 100 μg/ml RIF, 0.1 μg/ml CIP, and 20 μg/ml CHL. 
     Identification of SSAP Library Members 
     To generate Broad SSAP Library a multiple sequence alignment of eight SSAPs was used that had been shown to function in  E. coli  (Redβ, EF2132 from  Enterococcus faecalis , OrfC from  Legionella pneumophila,  5065 from  Vibrio cholerae , Plu2935 from  Photorhabdus luminescens , Orf48 from  Listeria monocytogenes , Orf245 from  Lactococcus lactis , and Bet from  Prochlorococcus siphovirus  P-SS2 (Datta et al., Proc. Natl. Acad. Sci. U.S.A. 105, 1626-1631 (2008); Sullivan et al., Environ. Microbiol. 11, 2935-2951 (2009))) to generate a Hidden Markov Model that described the weighted positional variance of these proteins. Then non-redundant nucleotide and environmental metagenomic databases were queried using a web-based search interface (Finn et al., Nucleic Acids Res. 39, W29-W37 (2011)). Candidates were filtered based on gene size and annotation. Those that exhibited intra-sequence similarity of greater than 98% were removed from the group. Three eukaryotic SSAP homologs were added to the library (Eisen et al., Proc. Natl. Acad. Sci. U.S.A. 85, 7481-7485 (1988)). In total, Broad SSAP Library contains 120 members from the homology search, 8 members from the starting sequence alignment, and 3 eukaryotic members, or a total of 131 SSAP homologs. 
     Broad RecT Library was generated from the full alignment of Pfam family PF03837, containing 576 sequences from Pfam 31.0 (El-Gebali et al., Nucleic Acids Res. 47, D427-D432 (2019)). Using ETE 3, a phylogenetic tree made by FastTree and accessed from the Pfam31.0 database was pruned, and from it a maximum diversity subtree of 100 members was identified (Huerta-Cepas et al., Mol. Biol. Evol. 33, 1635-1638 (2016)). Five members of this group were found in Library 51, and so were excluded, and in their place six RecT variants from  Streptomyces  phages and eight other RecT variants were added that had previously reported activity or were otherwise of interest (Zhang et al., Nat. Genet. 20, 123-128 (1998); Sun et al., Appl. Microbiol. Biotechnol. 99, 5151-5162 (2015); Datta et al., Proc. Natl. Acad. Sci. U.S.A. 105, 1626-1631 (2008); van Pijkeren et al., Bioengineered 3, 209-217 (2012); van Kessel et al., Nat. Methods 4, 147-152 (2007)), bringing the library size to 109. 
     Library Cloning 
     Diverse collections of SSAPs (PF03837) and SSBs (PF00436) were sourced from the Pfam database. A collection of ˜200 SSAPs was chosen to maximize for diversity of protein sequence. SSBs were then chosen from the organism (or a phylogenetically proximal organism) from which the SSAPs were sourced. Genes were codon-optimized for  E. coli  and synthesized by Twist Bioscience Corp. Genes were cloned into an entry vector by Gibson Assembly and then moved into vectors compatible with each of the three species by Golden Gate Assembly. 
     Broad SSAP Library and Broad RecT Library variants with a DNA barcode 22 nucleotides downstream of the stop codon were codon-optimized for  E. coli  and synthesized by Gen9 (S1) or Twist (S2). Synthesized DNA was amplified by PCR (NEB Q5 polymerase) and cloned into pDONR/Zeo (Thermo) by Gibson Assembly (NEB HiFi DNA Assembly Master Mix) and then moved into pARC8-DEST for arabinose-inducible expression. pARC8-DEST was engineered from a pARC8 plasmid (Choe et al., Biochem. Biophys. Res. Commun. 334, 1233-1240 (2005)) that shows good inducible expression in  E. coli  by moving Gateway sites (attR1/attR2), a CHL marker, and a ccdB counter-selection marker downstream of the pBAD-araC regulatory region ( FIGS. 10A-10B ). This enabled easy, one-step cloning of the entire library into pARC8-DEST by Gateway cloning (Thermo). The Gateway reaction was transformed into E. cloni Supreme electrocompetent cells (Lucigen), providing &gt;10,000× coverage of both libraries in total transformants. 
     Library Selection 
     Native resistance alleles were identified in each of the three species for resistance to rifampicin (rif) at the rpoB locus or streptomycin (stm) at the rpsL locus. The concentration of antibiotic necessary to confer a selective benefit to the resistant allele was determined for each strain. Libraries were transformed into the respective strains with at least 10× coverage, and ten successive cycles of MAGE editing followed by antibiotic selection were conducted to select for the SSAPs or SSAP/SSB pairs that most effectively conferred the antibiotic resistant allele via oligonucleotide-mediated recombineering. Rif and stm selections were performed in a non-resistant organism, and following these two rounds of selection, the plasmid library was mini-prepped and transformed back into the naïve parent strain. In this way, ten rounds of selection were performed two at a time. Fresh plasmid preparations and transformations were performed every two selection steps. In  E. coli  five different selective alleles were used, and so only one mini-prep and retransformation was necessary. 
     Libraries were mini-prepped (NEB Monarch Kit) and electroporated into the SEER chassis with more than 1,000-fold coverage. Five cycles of oligo-mediated recombineering followed by antibiotic selection were then conducted ( FIG. 1B ). 5 μl of the 5 ml recovery from the recombineering step was immediately plated onto LBL+selective antibiotic plates to estimate the total throughput of the selective step. This allowed us to ensure that the library was never bottlenecked—the first round of selection was the most stringent, but it was ensured that there was &gt;500× coverage at this stage. Following five rounds of selection, the plasmid library was mini-prepped and transformed back into the naïve parent strain, followed by five further rounds of selection (ten in total). After each selective step a 100 μl aliquot of the antibiotic-selected recovery was frozen down at −80° C. in 25% glycerol for analysis by NGS. 
     Efficiency Testing 
     The efficiency of each SSAP or SSAP/SSB pair was measured by expressing them off of their host-specific plasmid in the naïve parent strain and running a recombineering cycle with an oligo that confers a 4-nucleotide non-coding mismatch in a non-essential gene. The allele was then amplified by PCR and editing efficiency was measured by next-generation sequencing. 
     Next Generation Sequencing of Libraries 
     Primers were designed to amplify a 215 bp product containing the barcode region of the SSAP libraries from the pARC8 plasmid and to add on Illumina adaptors. PCR amplification was done with Q5 polymerase (NEB) performed on a LightCycler 96 System (Roche), with progress tracked by SYBR Green dye and amplification halted during the exponential phase. Barcoding PCR for Illumina library prep was performed as just described, but with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB). Barcoded amplicons were then purified with AMPure XP magnetic beads (Beckman Coulter), pooled, and the final pooled library was quantified with the NEBNext Library Quant Kit for Illumina (NEB). The pooled library was diluted to 4 nM, denatured, and a paired end read was run with a MiSeq Reagent Kit v3, 150 cycles (Illumina). Sequencing data was downloaded from Illumina, sequences were cleaned with Sickle (Joshi et al., Sickle: A sliding-window, adaptive, quality-based trimming tool for FastQ files. (2019)) and analyzed with custom scripts written in Python. 
     Measuring recombineering efficiency in  E. coli  by NGS 
     To measure single locus editing, a recombineering cycle was run with an oligo that confers a single base pair non-coding mismatch in a non-essential gene. The allele was then amplified by PCR and editing efficiency was measured by NGS as described above. To test multiplex editing, the concentration of oligo was held fixed (10 μM in the final electroporation mixture), but the total number of oligos in the mixture was varied. Pools of oligos to test editing at 5, 10, 15, or 20 alleles simultaneously were designed so as to space the edits relatively evenly around the genome. The 5-oligo pool contained oligo #&#39;s 3,7,11,15,17, the 10-oligo pool added oligo #&#39;s 1,5,9,13,19, the 15-oligo pool added oligo #&#39;s 4,8,12,16,18, and the final 20-oligo pool contained silent mismatch MAGE oligos. One locus (locus 8) showed major irregularities when sequenced, and so it was eliminated from the analysis. 
     DIvERGE-Based Simultaneous Mutagenesis of gyrA, gyrB, parE, and parC 
     A single round of DIvERGE mutagenesis was carried out to simultaneously mutagenize gyrA, gyrB, parE, and parC in  E. coli  MG1655 by the transformation of an equimolar mixture of 130 soft-randomized DIvERGE oligos, tiling the four target genes. The sequences and composition of these oligos were published previously (Nyerges, A., et. al, PNAS, 2018). To perform DIvERGE, 4 μl of this 100 μM oligo mixture was electroporated into  E. coli  K-12 MG1655 cells expressing Redβ from pORTMAGE311B, PapRecT from pORTMAGE312B, or CspRecT from pORTMAGE-Ec1, in 5 parallel replicates according to a previously described protocol (Szili et al., Antimicrob. Agents Chemother. AAC.00207-19 (2019)). Following electroporation, the replicates were combined into 10 ml fresh TB media. Following recovery for 2 hours, cells were diluted by the addition of 10 ml LB and allowed to reach stationary phase at 37° C., 250 rpm. Library generation experiments were performed in triplicates. Following library generation, 1 mL of outgrowth from each library was subjected to 250, 500, and 1,000 ng/mL ciprofloxacin (CIP) stresses on 145 mm-diameter LB-agar plates. Colony counts were determined after 72 hours of incubation at 37° C., and individual colonies were subjected to further genotypic (i.e., capillary DNA sequencing) analysis and phenotypic (i.e., Minimum Inhibitory Concentration) measurements. 
     pORTMAGE Plasmid Construction and Optimization 
     Cloning reactions were performed with Q5 High-Fidelity Master Mix and HiFi DNA Assembly Master Mix (New England Biolabs). pORTMAGE312B (Addgene plasmid) and pORTMAGE-Ec1 (Addgene plasmid) were constructed by replacing the Redβ open reading frame (ORF) of pORTMAGE311B plasmid (Addgene plasmid 120418) (Szili et al., bioRxiv 495630 (2018) doi:10.1101/495630) with PapRecT and CspRecT respectively. pORTMAGE-Pa1 was constructed in many steps: i.) the Kanamycin resistance cassette and the RSF1010 origin-of-replication on pORTMAGE312B with Gentamicin resistance marker and pBBR1 origin-of-replication, amplified from pSEVA631 (Martinez-Garcia et al., Nucleic Acids Res. 43, D1183-D1189 (2015)), ii.) optimization of RBSs in pORTMAGE-Pa1 was done by designing a 30-nt optimal RBS in front of the SSAP ORF and in between the SSAP and MutL ORFs with an automated design program, De Novo DNA (Salis et al., Nat. Biotechnol. 27, 946-950 (2009)), iii.) PaMutL was amplified from  Pseudomonas aeruginosa  genomic DNA and cloned in place of EcMutL_E32K, and finally iv.) PaMutL was mutated by site-directed mutagenesis to encode E36K. Ssr and Rec2 were ordered as gblocks from IDT and cloned in place of PapRecT into earlier versions of pORTMAGE-Pa1 for the comparisons in  FIG. 12 . 
     Measuring Recombineering Efficiency in Gammaproteobacteria by Selective Plating 
     Oligos were designed to introduce I) premature STOP codons into lacZ for  E. coli, K. pneumoniae , and  C. freundii , or II) RpsL K43→R; GyrA T83→I; ParC S83→L; RpoB D521→V, or a premature STOP codon into nfxB for  P. aeruginosa . Oligo-mediated recombineering was performed as described above on all bacterial strains. After recovery overnight, cells were plated at empirically-determined dilutions to a density of 200-500 colonies per plate. In the case of LacZ screening, plating was assayed on MacConkey agar plates or on X-Gal/IPTG LB L  agar plates in the case of  K. pneumoniae . In the case of selective antibiotic screening, cultures were plated onto both selective and non-selective plates. Selective antibiotic concentrations used were the same as those described for the selective testing above, except that in  P. aeruginosa  100 μg/ml STR and 1.5 μg/ml CIP were used unless otherwise noted. Variants that were resistant to multiple antibiotics were selected on LB L  agar plates that contained the combination of all corresponding antibiotics. Non-selective plates were antibiotic-free LB L  agar plates. In all cases, allelic-replacement frequencies were calculated by dividing the number of recombinant CFUs by the number of total CFUs. Plasmid maintenance was ensured by supplementing all media and agar plates with either KAN (50 μg/ml) or GEN (20 μg/ml).
 
Minimum Inhibitory Concentration (MIC) Measurement in  P. aeruginosa  
 
     MICs were determined using a standard serial broth microdilution technique according to the CLSI guidelines (ISO 20776-1:2006, Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases). Briefly, bacterial strains were inoculated from frozen cultures onto MHBII agar plates and were grown overnight at 37° C. Next, independent colonies from each strain were inoculated into 1 ml MHBII medium and were propagated at 37° C., 250 rpm overnight. To perform MIC tests, 12-step serial dilutions using 2-fold dilution-steps of the given antibiotic were generated in 96-well microtiter plates (Sarstedt 96-well microtest plate). Antibiotics were diluted in 100 μl of MHBII medium. Following dilutions, each well was seeded with an inoculum of 5×10 4  bacterial cells. Each measurement was performed in 3 parallel replicates. Plates were incubated at 37° C. under continuous shaking at 150 rpm for 18 hours in an INFORS HT shaker. After incubation, the OD 600  of each well was measured using a Biotek Synergy 2 microplate reader. MIC was defined as the antibiotic concentration which inhibited the growth of the bacterial culture, i.e., the drug concentration where the average OD600 increment of the three replicates was below 0.05. 
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         8 Mosberg, J. A., Gregg, C. J., Lajoie, M. J., Wang, H. H. &amp; Church, G. M. Improving lambda red genome engineering in  Escherichia coli  via rational removal of endogenous nucleases. PloS one 7, doi:10.1371/journal.pone.0044638 (2012). 
         9 Lajoie, M. J. et al. Genomically recoded organisms expand biological functions. Science (New York, N.Y.) 342, 357-360, doi:10.1126/science.1241459 (2013). 
         10 Mandell, D. J. et al. Biocontainment of genetically modified organisms by synthetic protein design. Nature 518, 55-60, doi:10.1038/nature14121 (2015). 
         11 Pirman, N. L. et al. A flexible codon in genomically recoded  Escherichia coli  permits programmable protein phosphorylation. Nature Communications, doi:10.1038/ncomms9130 (2015). 
         12 Wannier, T. M. et al. Adaptive evolution of genomically recoded  Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 115, 3090-3095, doi:10.1073/pnas.1715530115 (2018). 
         13 Wang, H. H. et al. Programming cells by multiplex genome engineering and accelerated evolution. Nature 460, 894 (2009). 
         14 Ioerger, T. R. et al. Identification of new drug targets and resistance mechanisms in  Mycobacterium tuberculosis . PLOS ONE, doi:10.1371/journal.pone.0075245 (2013). 
         15 Pijkeren, J.-P. &amp; Britton, R. A. High efficiency recombineering in lactic acid bacteria. Nucleic Acids Research 40, doi:10.1093/nar/gks147 (2012). 
         16 Huttenhower, C. et al. Structure, function and diversity of the healthy human microbiome. Nature 486, 207-214, doi:10.1038/nature11234 (2012). 
         17 Keseler, I. M. et al. The EcoCyc database: reflecting new knowledge about  Escherichia coli  K-12. Nucleic Acids Research 45, doi:10.1093/nar/gkw1003 (2016). 
         18 Yarza, P. et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nature Reviews Microbiology 12, 635, doi:10.1038/nrmicro3330 (2014). 
         19 Opijnen, V. T., Bodi, K. L. &amp; Camilli, A. Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms. Nature Methods 6 (2009). 
         20 Gilbert, L. A. et al. Genome-scale CRISPR-mediated control of gene repression and activation. Cell (2014). 
         21 Baba, T. et al. Construction of  Escherichia coli  K-12 in-frame, single-gene knockout mutants: the Keio collection. Molecular Systems Biology (2006). 
         22 Price, M. N. et al. Mutant phenotypes for thousands of bacterial genes of unknown function. Nature 557, 503-509, doi:10.1038/s41586-018-0124-0 (2018). 
         23 Pawluk, A. et al. Inactivation of CRISPR-Cas systems by anti-CRISPR proteins in diverse bacterial species. Nature Microbiology 1, 16085, doi:10.1038/nmicrobiol.2016.85 (2016). 
         24 Evers, B. et al. CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes. Nature Biotechnology, doi:10.1038/nbt.3536 (2016). 
         25 Macosko, E. Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 161, 1202-1214 (2015). 
         26 Kizer, L., Pitera, D. J., Pfleger, B. F. &amp; Keasling, J. D. Application of functional genomics to pathway optimization for increased isoprenoid production. Applied and Environmental Microbiology 74, 3229-3241, doi:10.1128/AEM.02750-07 (2008). 
         27 Kunjapur, A. M. &amp; Prather, K. L. J. Microbial Engineering for Aldehyde Synthesis. Applied and Environmental Microbiology 81, 1892-1901, doi:10.1128/AEM.03319-14 (2015). 
         28 Li, Q., Du, W. &amp; Liu, D. Perspectives of microbial oils for biodiesel production. Applied Microbiology and Biotechnology 80, 749-756, doi:10.1007/s00253-008-1625-9 (2008). 
         29 Kunjapur, A. M., Tarasova, Y. &amp; Prather, K. L. J. Synthesis and Accumulation of Aromatic Aldehydes in an Engineered Strain of  Escherichia coli . Journal of the American Chemical Society 136, 11644-11654, doi:10.1021/ja506664a (2014). 
         30 Pitera, D. J., Paddon, C. J., Newman, J. D. &amp; Keasling, J. D. Balancing a heterologous mevalonate pathway for improved isoprenoid production in  Escherichia coli . Metabolic engineering 9 (2007). 
         31 Temme, K., Zhao, D. &amp; Voigt, C. A. Refactoring the nitrogen fixation gene cluster from  Klebsiella oxytoca . Proceedings of the National Academy of Sciences of the United States of America 109, 7085-7090, doi:10.1073/pnas.1120788109 (2012). 
         32 Wang, L. et al. A minimal nitrogen fixation gene cluster from  Paenibacillus  sp. WLY78 enables expression of active nitrogenase in  Escherichia coli . PLoS Genetics 9, doi:10.1371/journal.pgen.1003865 (2013). 
         33 Shiba, Y., Paradise, E. M., Kirby, J., Dai-Kyun, R. &amp; Keasling, J. D. Engineering of the pyruvate dehydrogenase bypass in  Saccharomyces cerevisiae  for high-level production of isoprenoids. Metabolic Engineering 9, 160-168 (2007). 
         34 Wang, H. H. et al. Genome-scale promoter engineering by coselection MAGE. Nature Methods 9, 591-593, doi:10.1038/nmeth.1971 (2012). 
         35 Isaacs, F. J. et al. Precise manipulation of chromosomes in vivo enables genome-wide codon replacement. Science (New York, N.Y.) 333, 348-353, doi:10.1126/science.1205822 (2011). 
         36 Chmielewska-Jeznach, M., Bardowski, J. K. &amp; Szczepankowska, A. K. Molecular, physiological and phylogenetic traits of  Lactococcus  936-type phages from distinct dairy environments. Scientific reports 8, 12540, doi:10.1038/s41598-018-30371-3 (2018). 
       
    
     Example 10 
     To understand the host-tropism displayed by RecT&#39;s, a simplified in-vitro model of oligonucleotide annealing that includes bacterial SSBs, a key host protein that coats ssDNA at the replication fork was developed. It was first whether two 90 bp oligos could anneal if they were pre-coated with SSB. SSBs was purified from  E. coli  (gram-negative), where most recombineering work has been performed, and  L. lactis  (gram-positive), a lactic acid bacterium phylogenetically distantly related to  E. coli . Using fluorescence quenching to measure annealing, it was found that while the free oligos annealed together slowly ( FIGS. 17A-17B ), both EcSSB and L1SSB completely inhibited oligonucleotide annealing. It was next tested capacity of a phage RecT protein to overcome this SSB-mediated inhibition of annealing. Thus, Redβ, which is not broadly portable, but mediates efficient oligonucleotide annealing in  E. coli , was purified. It was found that adding Redβ overcame the inhibitory effect of EcSSB but not L1SSB, rapidly annealing the EcSSB-coated two oligos together ( FIGS. 17A and 17C ). These preliminary results gave us an indication that while bacterial SSBs inhibit oligonucleotide annealing in vitro, RecTs overcome the inhibitory effect in an SSB-specific manner. 
     To validate this result in vivo, an assay was developed to measure the portability of RecT proteins. Four variants known to enable high genome editing efficiency were selected (Redβ and PapRecT in  E. coli , LrpRecT in  L. lactis , and MspRecT in  M. smegmatis ), and tested codon optimized versions of all four in both  E. coli  (gram-negative), and  L. lactis  (gram-positive). Genome editing efficiency was measured by introducing oligos encoding known antibiotic resistance mutations, and compared the antibiotic resistant cell counts to the total number of viable cells in the population ( FIGS. 17E-17F ). In  E. coli , Redβ and PapRecT functioned well, and improved oligo incorporation 1600-fold and 2700-fold respectively, while MspRecT (290-fold improvement) and LrpRecT (5.6-fold improvement) were less effective ( FIG. 17G ). In  L. lactis , LrpRecT was the only functional homolog, and improved oligo incorporation 7,700-fold, while the three other RecT variants were nearly non-functional, improving oligo incorporation less than 7-fold ( FIG. 17H ). No RecT protein functioned well both in  E. coli  and  L. lactis . This agrees with previous studies, which have found that RecT proteins are usually not portable between distantly related bacterial species. 
     If interaction with the bacterial SSB is required for phage RecT functionality, one solution to establishing recombineering in a new species would be to replace the host SSB with one compatible with the chosen RecT. However, SSB proteins are essential, and mutations to SSB can result in severe growth defects. Therefore, it was evaluated if temporary overexpression of an exogenous SSB could supply the necessary requirements for recombineering and improve the activity of non-host compatible RecTs. SSBs corresponding to each RecT protein were synthesized and tested the activity of all four cognate RecT-SSB pairs in  L. lactis  and  E. coli . Co-expression of a cognate bacterial SSB improved the genome editing efficiencies of all RecTs with low host-compatibility ( FIGS. 17G-17H ). The best performing pairs, Redβ+EcSSB and PapRecT+PaSSB demonstrated 483-fold and 1,168-fold improved editing efficiencies over the RecT proteins alone in  L. lactis , and still maintained high activity in  E. coli  ( FIGS. 17G-17H ). In  E. coli , co-expression with cognate SSBs also significantly reduced the toxicity of these two pairs (data not shown). These results, especially in  L. lactis , indicate that the presence of a cognate bacterial SSB can overcome the host incompatibility of RecT proteins if moved to new species. 
     Example 11 
     It was next investigated which domains on SSB were involved in mediating the RecT protein interaction. A SSB domain-specific model for understanding RecT protein portability would be far more informative than previous models, which relied on phylogenetic relationships between the host organisms. RecT proteins have been shown to function in species with SSBs with relatively divergent sequences. Therefore, there was interest in identifying conserved domains responsible for maintaining the RecT protein interaction. For example, while Redβ works well in  E. coli, Salmonella enterica , and  Citrobacter freundii  which have SSBs with 88% identity, PapRecT works in  E. coli  and  Pseudomonas aeruginosa , which have SSBs of only 59% identity. To investigate the specific residues involved, the genome editing assay was used in  L. lactis  and the effect of co-expressing RecT proteins with non-cognate or mutated SSBs was evaluated. 
     The importance of the SSB C-terminal tail was evaluated by coexpressing Redβ in  L. lactis , along with a version of EcSSB that had a 9-amino-acid C-terminal deletion (EcSSBΔ9) ( FIG. 18A ). In  L. lactis , the genome editing efficiency of Redβ with EcSSBΔ9 was 44-fold lower than Redβ with EcSSB, indicating a key role for the C-terminal domain in the SSB-mediated efficiency improvement ( FIG. 18 c   ). Next, Redβ was co-expressed with the  L. lactis  SSB (L1SSB). Co-expression of Redβ with L1SSB performed similarly to Redβ with EcSSBΔ9, and improved genome editing efficiency 38.5-fold less than Redβ with EcSSB. Then, Redβ was co-expressed with chimeric versions of the L1SSB, where up to 9 amino acids of the L1SSB C-terminal tail were replaced with their corresponding residues from EcSSB. Swapping the last 7 C-terminal residues (L1SSB C7:EcSSB) improved editing rates to within 5.9-fold of Redβ with EcSSB, and swapping the last 8 C-terminal residues (L1SSB C8:EcSSB) improved editing rates within 2.6-fold of Redβ with EcSSB. These results support a model where Redβ specifically recognizes at minimum the 7 C-terminal acids of  E. coli  SSB, but not that of  L. lactis  SSB. 
     To evaluate if the SSB C-terminal 7 amino acids also affected the compatibility of the other two non-host compatible RecT proteins, similar SSB-chimera experiments were performed with PapRecT and MspRecT in  L. lactis . The genome editing efficiency of PapRecT with the  L. lactis  SSB was 135-fold less than when using the cognate pair ( FIG. 18E ). However, this defect was completely recovered when PapRecT was co-expressed with  L. lactis  SSB chimeras where either the last 7 or 8 C-terminal resides were replaced (L1SSB C7:PaSSB, L1SSB C8:PaSSB) ( FIG. 18E ). For MspRecT, the genome editing efficiency with L1SSB was 33-fold lower than when using the cognate pair ( FIG. 18F ). Again, the defect was completely recovered when MspRecT was co-expressed with  L. lactis  SSB chimeras where either the last 7 or 8 C-terminal resides were replaced (L1SSB C7:MsSSB, L1SSB C8:MsSSB) ( FIG. 18F ). Since the chimeric L1SSBs greatly improved the functionality of non-host compatible RecT proteins, while the wild-type L1SSB did not, the RecT-SSB interaction seems to be both specific and relatively modular, with the 7 C-terminal amino acids acting as the critical interaction domain. 
     These results provide a molecular basis for the portability of RecT proteins between species which have host SSBs with a conserved C-terminal tail. Specifically, although the SSBs have only 59% identity, the  P. aeruginosa  and  E. Coli  SSBs have a perfectly conserved 7 amino acid C-terminal tail domain ( FIG. 19C ), supporting the functionality of PapRecT in  E. coli . Additionally,  E. coli, Salmonella enterica  and  Citrobacter freundii  SSBs all have a perfectly conserved 7 amino acid C-terminal tails, supporting the portability of Redβ between these species ( FIG. 19C ). 
     Example 12 
     Some RecT proteins are known to be portable between species which have distinct SSB C-terminal tails. To better characterize the network of RecT-SSB compatibility among the proteins analyzed here, all four RecTs were co-expressed with all four SSBs in both  E. coli  and  L. lactis  ( FIGS. 19A-19B ). It was found that the effects of PaSSB and EcSSB on RecT-mediated editing efficiency were relatively interchangeable, as might be expected since they share the same 7 amino acid C-terminal tail ( FIG. 19C ). Interestingly, PapRecT displayed the characteristics of a more portable RecT protein, and showed compatibility with MsSSB and EcSSB/PaSSB, even though their 7AA C-terminal tail sequences are distinct ( FIGS. 19A and 19C ). Importantly, co-expressing PapRecT with LrSSB did not provide a substantial improvement in genome editing efficiency in  L. lactis , even though the 7 C-terminal tail amino acids of LrSSB differ only by a single residue from MsSSB ( FIGS. 19A and 19C ). 
     To test if PapRecT specifically interacts with the C-terminal tail of MsSSB, PapRecT was co-expressed with a chimeric version of LrSSB, with either the C7 or C8 amino acids matching that of MsSSB ( FIG. 19D ). The chimeric constructs demonstrated the same editing efficiency as PapRecT+MsSSB, showing that a single amino acid change was sufficient to enable compatibility between the proteins ( FIG. 19D ). The compatibility of PapRecT with the distinct EcSSB/PaSSB and MsSSB tails but not the LrSSB tail affirms that while the SSB C-terminal tail has a critical role in the RecT-SSB interaction, there can be flexibility in the specific motif recognized. 
     We next evaluated if the interaction between PapRecT and MsSSB in  L. lactis  indicated that PapRecT would function in  M. smegmatis , where MsSSB is natively expressed. All four RecT&#39;s were tested in this species and indeed found that in  M. smegmatis  PapRecT enabled high efficiency editing, incorporating oligos at the same rate as MspRecT, while the other two RecT variants had much lower efficiency ( FIG. 19E ). 
     Finally, the model for RecT was used to establish recombineering in  L. rhamnosus , a well-studied probiotic used to treat a variety of illnesses including diarrhea and bacterial vaginosis. Although the  L. rhamnosus  SSB and  L. lactis  SSB only have 47% identity, they share identical SSB C-terminal tail 7 amino acids. It was determined whether LrpRecT (which functions in  L. lactis ) is portable to  L. rhamnosus , while the other RecT proteins would not be functional. The 4 RecT proteins were tested in this species and it was found that LrpRecT incorporated oligonucleotides three orders of magnitude above the background level, while Redβ and PapRecT had negligible activity and MspRecT was toxic. 
     Example 13 
     In  L. lactis , the co-expression of PapRecT and Redβ with compatible SSB&#39;s improved genome editing efficiency to a level comparable with the host-compatible LrpRecT. It was determined whether for some species, RecT-SSB pairs could provide functional recombineering capacity even if no functional RecT protein had previously been identified. Therefore, the two best-performing RecT-SSB pairs (Redβ+PaSSB, and PapRecT+PaSSB) were tested for activity in  Caulobacter crescentus , a model organism for studying cell cycle regulation, replication, and differentiation. 
     In  C. crescentus , no significant editing enhancement was detected over the background with the RecT proteins alone, or PapRecT+PaSSB. As compatibility between PapRecT and PaSSB was previously observed, it seemed likely that additional factors must contribute to the incompatibility of this pair with  C. crescentus . However, using Redβ+PaSSB, a 15-fold improvement over Redβ alone was observed ( FIG. 20A ). After expression optimization ( FIG. 22 ) and evasion of mismatch repair, Redβ+PaSSB demonstrated 873-fold improved editing efficiency over the background level, which was 112-fold higher than Redβ alone ( FIG. 20B ). These results indicate that while RecT-SSB pairs are not universally portable (data not shown), the co-expression of a RecT protein with a compatible bacterial SSB will equal or surpass the editing efficiencies of RecT proteins alone. 
     Example 14 
     In  E. coli , one of the unique capabilities of recombineering is the ability to generate rationally designed or high-coverage genomic libraries. Although this technique (termed MAGE) has been used for a variety of applications including optimizing metabolic pathways, protein evolution, and saturation mutagenesis, it has only been used in a limited capacity in other species.  L. lactis , a microbe distantly related to  E. coli , was used to demonstrate how mismatch repair evasion and oligonucleotide library design can be used to perform high-coverage genomic mutagenesis after a functional RecT protein has been identified. 
     To begin, the assay in  L. lactis  was adapted to allow the efficient incorporation of single, double, or triple nucleotide mutations, which are normally recognized and corrected by mismatch repair pathways. The cognate pair PapRecT and PaSSB, was used and co-expressed either the dominant negative mismatch repair protein MutL.E32K from  E. coli , or the host protein  L. lactis  MutL carrying the equivalent mutation (L1MutL.E33K, data not shown). While MutL.E32K from  E. coli  was nonfunctional, co-expression of LlMutL.E33K enabled the efficient introduction of 1 bp pair changes ( FIGS. 23A-23E ). Optimization of inducer and oligonucleotide concentrations further improved editing efficiency 26-fold ( FIGS. 23A-23E ). 
     Table 3 includes sequences that were used in Examples 10-14. 
     See also, e.g., Filsinger et al., bioRxiv 2020.04.14.041095 (doi.org/10.1101/2020.04.14.041095), which is herein incorporated by reference in its entirety. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Additional sequences 
               
            
           
           
               
               
               
               
            
               
                 Gene or 
                 Codon 
                   
                   
               
               
                 construct 
                 optimized 
                   
                 SEQ ID 
               
               
                 name 
                 for: 
                 Sequence 
                 NO: 
               
               
                   
               
               
                 λBeta 
                   L.   lactis ; 
                 ATGAGTACTGCACTTGCAACATTAGCTGGCAAGTTAGCAGAG 
                 473 
               
               
                   
                   L.   rhamnosus   
                 CGTGTTGGTATGGATTCAGTCGACCCTCAGGAGCTTATAACT 
                   
               
               
                   
                   
                 ACCTTACGTCAAACAGCGTTCAAGTGTGACGCCTCTGATGCA 
                   
               
               
                   
                   
                 CAATTTATCGCTTTGCTTATCGTAGCTAACCAGTATGGGTTGA 
                   
               
               
                   
                   
                 ATCCTTGGACGAAGGAGATATACGCTTTCCCGGATAAGCAGA 
                   
               
               
                   
                   
                 ACGGTATTGTTCCTGTAGTAGGTGTCGATGGATGGAGTAGAA 
                   
               
               
                   
                   
                 TTATCAATGAAAATCAACAGTTCGATGGCATGGACTTCGAGC 
                   
               
               
                   
                   
                 AGGATAATGAATCATGTACCTGCCGTATATATAGAAAAGACC 
                   
               
               
                   
                   
                 GAAATCACCCAATTTGTGTGACTGAATGGATGGATGAGTGCA 
                   
               
               
                   
                   
                 GACGTGAGCCGTTCAAGACCCGAGAAGGCCGTGAAATCACTG 
                   
               
               
                   
                   
                 GTCCGTGGCAATCACATCCAAAGAGAATGTTGCGTCACAAGG 
                   
               
               
                   
                   
                 CGATGATTCAGTGCGCCCGTTTAGCTTTTGGGTTTGCTGGCAT 
                   
               
               
                   
                   
                 TTACGACAAGGACGAAGCTGAAAGAATCGTTGAAAACACTG 
                   
               
               
                   
                   
                 CATATACCGCTGAACGACAACCGGAGCGTGACATTACGCCAG 
                   
               
               
                   
                   
                 TGAATGACGAGACAATGCAGGAAATTAACACGTTGTTGATTG 
                   
               
               
                   
                   
                 CTTTGGACAAAACGTGGGACGACGACTTGTTACCACTTTGTA 
                   
               
               
                   
                   
                 GCCAAATTTTTCGTCGAGACATTAGAGCTTCATCTGAGCTTAC 
                   
               
               
                   
                   
                 ACAAGCTGAAGCCGTCAAGGCATTGGGGTTTTTGAAACAAAA 
                   
               
               
                   
                   
                 AGCTACCGAACAGAAGGTAGCGGCATAA 
                   
               
               
                   
               
               
                 PapRecT 
                   L.   lactis ; 
                 ATGGGAACCGCCCTTACACCTCTTTTGACAAAGTTCGCAACC 
                 476 
               
               
                   
                   L.   rhamnosus   
                 AGATATGAGATGGGAACGACCCCTGAAGAGGTTGCTAATACA 
                   
               
               
                   
                   
                 TTGAAACAAACATGCTTCAAGGGACAGGTCAACGACAGTCAA 
                   
               
               
                   
                   
                 ATGGTAGCCCTTTTGATAGTCGCTGACCAGTACAAGTTAAAC 
                   
               
               
                   
                   
                 CCGTTCACCAAGGAGTTGTATGCATTCCCTGACAAGAATAAT 
                   
               
               
                   
                   
                 GGAATCGTGCCAGTTGTTGGTGTCGATGGATGGGCGAGAATA 
                   
               
               
                   
                   
                 ATAAACGAGAACCCTCAGTTTGATGGTATGGAATTTTCTATG 
                   
               
               
                   
                   
                 GACCAGCAGGGCACTGAGTGCACGTGCAAAATCTATCGTAAG 
                   
               
               
                   
                   
                 GATCGTTCTCACGCAATAAGCGCTACGGAATATATGGCCGAA 
                   
               
               
                   
                   
                 TGTAAGAGAAATACGCAACCTTGGCAAAGTCACCCACGACGT 
                   
               
               
                   
                   
                 ATGTTAAGACATAAAGCCATGATCCAGTGTGCGCGATTAGCA 
                   
               
               
                   
                   
                 TTCGGCTTCGCTGGTATCTACGATCAAGACGAAGCGGAACGA 
                   
               
               
                   
                   
                 ATAGTCGAAAGAGACGTTACTCCGGCGGAGCAGTACGAAGA 
                   
               
               
                   
                   
                 TGTCAGCGAAGCTATATGCTTGATTAAGGACAGCCCGACGAT 
                   
               
               
                   
                   
                 GGAGGATTTACAGGCAGCGTTCAGCAATGCCTGGAAGGCGTA 
                   
               
               
                   
                   
                 TAAAACCAAAGGTGCAAGAGACCAATTGACAGCCGCCAAGG 
                   
               
               
                   
                   
                 ATCAGCGTAAAAAGGAATTACTTGATGCCCCAATAGATGTCG 
                   
               
               
                   
                   
                 AGTTCGAAGAAACTGGCGATGATAGAGCAGCATAA 
                   
               
               
                   
               
               
                 MspRecT 
                   L.   lactis ; 
                 ATGGCAGAAAACGCCGTGACGAAACAAGACTCACCTAAAGC 
                 477 
               
               
                   
                   L.   rhamnosus   
                 CCCAGAAACGATATCACAGGTCCTTCAAGTGTTAGTACCTCA 
                   
               
               
                   
                   
                 ATTAGCTCGAGCCGTACCTAAGGGAATGGATCCTGATAGAAT 
                   
               
               
                   
                   
                 AGCTCGAATCGTCCAGACCGAGATCAGAAAGAGTAGAAATG 
                   
               
               
                   
                   
                 CGAAAGCGGCGGGAATCGCCAAACAGTCATTGGATGACTGC 
                   
               
               
                   
                   
                 ACGCAAGAAAGCTTCGCCGGGGCGTTACTTACAAGTGCGGCA 
                   
               
               
                   
                   
                 TTGGGCCTTGAGCCAGGCGTTAACGGTGAGTGTTATCTTGTTC 
                   
               
               
                   
                   
                 CATACAGAGACACAAGAAGAGGTGTCGTCGAGTGCCAGTTA 
                   
               
               
                   
                   
                 ATTATCGGGTATCAAGGAATCGTTAAATTGTTTTGGCAACATC 
                   
               
               
                   
                   
                 CGCGAGCCTCTCGAATAGACGCCCAGTGGGTGGGGGCAAAC 
                   
               
               
                   
                   
                 GATGAATTCCATTATACAATGGGTCTTAACCCAACCTTAAAA 
                   
               
               
                   
                   
                 CATGTAAAGGCTAAGGGAGACCGAGGAAACCCAGTATATTTT 
                   
               
               
                   
                   
                 TATGCTATCGTAGAGGTCACGGGTGCCGAGCCTTTGTGGGAT 
                   
               
               
                   
                   
                 GTCTTCACAGCTGATGAGATTAGAGAGTTGAGAAGAGGTAAA 
                   
               
               
                   
                   
                 GTCGGTTCAAGCGGGGATATTAAGGATCCGCAGAGATGGATG 
                   
               
               
                   
                   
                 GAGCGAAAGACAGCGTTAAAACAAGTGCTTAAGCTTGCTCCA 
                   
               
               
                   
                   
                 AAAACTACTCGTTTGGACGCAGCAATACGAGCGGACGATAGA 
                   
               
               
                   
                   
                 CCGGGAACAGATTTGTCTCAAAGCCAGGCGTTGGCATTACCT 
                   
               
               
                   
                   
                 AGTACAGTTAAGCCAACAGCAGACTACATAGACGGTGAGATT 
                   
               
               
                   
                   
                 GCAGAGCCACACGAGGTTGACACTCCGCCTAAAAGCAGTCGA 
                   
               
               
                   
                   
                 GCACAACGAGCTCAGAGAGCGACTGCCCCAGCCCCAGACGTT 
                   
               
               
                   
                   
                 CAGATGGCCAATCCGGATCAATTAAAGCGTTTGGGAGAGATT 
                   
               
               
                   
                   
                 CAAAAAGCCGAAAAGTACAATGATGCCGACTGGTTTAAGTTC 
                   
               
               
                   
                   
                 TTAGCTGATAGTGCAGGGGTGAAAGCGACAAGAGCAGCTGA 
                   
               
               
                   
                   
                 TCTTACATTTGATGAAGCAAAAGCTGTAATAGATATGTTCGA 
                   
               
               
                   
                   
                 CGGCCCAAATGCTTGA 
                   
               
               
                   
               
               
                 LrpRecT 
                   L.   lactis ; 
                 ATGGCTAATCAAGTAGCACAACAGCAGAAACCGACTAAGCT 
                 478 
               
               
                   
                   L.   rhamnosus   
                 AACCGATCTTGTATTAGATCGTGTTAAACAAATGCAAGACAC 
                   
               
               
                   
                   
                 GCAGGACTTGTCACTACCCAAGAATTACAACGCTTCTAATGC 
                   
               
               
                   
                   
                 GTTGAATGCAGCCTTTCTCGAATTACAAAAAGTACAAGACCG 
                   
               
               
                   
                   
                 TAATCATCGGCCAGCCTTAGAAGTATGTTCTCATGACTCGATT 
                   
               
               
                   
                   
                 GTTAAGTCCTTGTTAGATATGACACTGCAAGGGCTATCCCCA 
                   
               
               
                   
                   
                 GCAAAAGATCAATGCTACTTCATCGTATACGGCAATGAGCTT 
                   
               
               
                   
                   
                 CAAATGCAACGGAGCTATTTCGGTACTGTTGCAGCAGTTAAG 
                   
               
               
                   
                   
                 CGACTGGATGGTGTTAAGAAAGTTAGGGCAGAAGTTGTTCAC 
                   
               
               
                   
                   
                 GAAAAAGATGACTTTGAAATTGGTGCTAATGAAGACATGGAG 
                   
               
               
                   
                   
                 CTAGTCGTTAAGAGGTTCGTTCCTAAGTTTGAAAATCAAGAT 
                   
               
               
                   
                   
                 AATCAAATTATTGGAGCTTTTGCCATGATTAAGACTGATGAA 
                   
               
               
                   
                   
                 GGTACTGACTTTACTGTTATGACTAAGAAAGAGATTGATCAG 
                   
               
               
                   
                   
                 TCATGGGCACAAACACGTCAAAAAAATAACAAAGTACAGCA 
                   
               
               
                   
                   
                 GAATTTTAGCCAAGAAATGGCAAAGCGTACTGTGCTTAATCG 
                   
               
               
                   
                   
                 TGCCGCTAAGATGTTTATTAACACGTCTGATGATAGTGACCTT 
                   
               
               
                   
                   
                 TTAACTGGTGCTATCAACGATACAACAAGCAACGAATACGAT 
                   
               
               
                   
                   
                 GATGAGCGTCGAGATGTAACGCCCGTTGAGGATGAAAAACA 
                   
               
               
                   
                   
                 AAGTACTGATAAATTGCTAGAAGGATTTCAAAAGTCACAAGA 
                   
               
               
                   
                   
                 AGCGAAGGCTAAGTGGGTAAGTAATGATGGCAACAGCAACG 
                   
               
               
                   
                   
                 AAGGCAAAGAAACCAGTGAAGAAGTCGCAGACGGACAAACA 
                   
               
               
                   
                   
                 GAACTCTTCAGCGAAGGGACAATCAAACCAGCCGATGAAGCT 
                   
               
               
                   
                   
                 GACAGCTAA 
                   
               
               
                   
               
               
                 λ Beta 
                   E.   coli   
                 ATGAGTACTGCACTCGCAACGCTGGCTGGGAAGCTGGCTGAA 
                 479 
               
               
                   
                   
                 CGTGTCGGCATGGATTCTGTCGACCCACAGGAACTGATCACC 
                   
               
               
                   
                   
                 ACTCTTCGCCAGACGGCATTTAAAGGTGATGCCAGCGATGCG 
                   
               
               
                   
                   
                 CAGTTCATCGCATTACTGATCGTTGCCAACCAGTACGGCCTTA 
                   
               
               
                   
                   
                 ATCCGTGGACGAAAGAAATTTACGCCTTTCCTGATAAGCAGA 
                   
               
               
                   
                   
                 ATGGCATCGTTCCGGTGGTGGGCGTTGATGGCTGGTCCCGCA 
                   
               
               
                   
                   
                 TCATCAATGAAAACCAGCAGTTTGATGGCATGGACTTTGAGC 
                   
               
               
                   
                   
                 AGGACAATGAATCCTGTACATGCCGGATTTACCGCAAGGACC 
                   
               
               
                   
                   
                 GTAATCATCCGATCTGCGTTACCGAATGGATGGATGAATGCC 
                   
               
               
                   
                   
                 GCCGCGAACCATTCAAAACTCGCGAAGGCAGAGAAATCACG 
                   
               
               
                   
                   
                 GGGCCGTGGCAGTCGCATCCCAAACGGATGTTACGTCATAAA 
                   
               
               
                   
                   
                 GCCATGATTCAGTGTGCCCGTCTGGCCTTCGGATTTGCTGGTA 
                   
               
               
                   
                   
                 TCTATGACAAGGATGAAGCCGAGCGCATTGTCGAAAATACTG 
                   
               
               
                   
                   
                 CATACACTGCAGAACGTCAGCCGGAACGCGACATCACTCCGG 
                   
               
               
                   
                   
                 TTAACGATGAAACCATGCAGGAGATTAACACTCTGCTGATCG 
                   
               
               
                   
                   
                 CCCTGGATAAAACATGGGATGACGACTTATTGCCGCTCTGTT 
                   
               
               
                   
                   
                 CCCAGATATTTCGCCGCGACATTCGTGCATCGTCAGAACTGA 
                   
               
               
                   
                   
                 CACAGGCCGAAGCAGTAAAAGCTCTTGGATTCCTGAAACAGA 
                   
               
               
                   
                   
                 AAGCCGCAGAGCAGAAGGTGGCAGCATGA 
                   
               
               
                   
               
               
                 PapRecT 
                   E.   coli   
                 ATGGGTACTGCTCTAACGCCGTTATTAACCAAGTTTGCCACCC 
                 480 
               
               
                   
                   
                 GCTATGAGATGGGAACTACCCCCGAAGAGGTCGCTAATACGC 
                   
               
               
                   
                   
                 TGAAACAGACTTGTTTCAAGGGCCAAGTGAACGATAGTCAGA 
                   
               
               
                   
                   
                 TGGTAGCCCTTTTGATCGTTGCGGATCAATATAAGCTCAATCC 
                   
               
               
                   
                   
                 ATTCACAAAAGAGCTCTACGCGTTCCCTGACAAAAATAATGG 
                   
               
               
                   
                   
                 TATTGTTCCAGTTGTGGGAGTCGATGGTTGGGCTAGAATTATT 
                   
               
               
                   
                   
                 AACGAGAATCCCCAGTTTGATGGGATGGAATTCAGTATGGAT 
                   
               
               
                   
                   
                 CAACAGGGAACTGAATGCACTTGTAAAATTTACCGCAAAGAC 
                   
               
               
                   
                   
                 CGCTCGCACGCCATCAGCGCCACCGAGTACATGGCTGAGTGC 
                   
               
               
                   
                   
                 AAAAGGAACACTCAACCTTGGCAGTCTCACCCGCGACGTATG 
                   
               
               
                   
                   
                 CTGCGTCATAAGGCTATGATTCAATGCGCCAGACTAGCCTTT 
                   
               
               
                   
                   
                 GGTTTCGCGGGGATCTACGATCAGGATGAGGCCGAACGCATT 
                   
               
               
                   
                   
                 GTTGAACGAGATGTAACTCCCGCCGAGCAATACGAGGATGTA 
                   
               
               
                   
                   
                 TCCGAAGCGATTTGTCTGATCAAAGACTCACCAACTATGGAG 
                   
               
               
                   
                   
                 GACTTGCAGGCCGCGTTCTCAAACGCGTGGAAAGCTTACAAG 
                   
               
               
                   
                   
                 ACTAAAGGTGCCCGTGATCAACTGACTGCTGCTAAAGACCAG 
                   
               
               
                   
                   
                 AGAAAAAAGGAGCTGTTGGATGCGCCCATTGATGTCGAATTC 
                   
               
               
                   
                   
                 GAAGAAACTGGAGATGATCGTGCTGCGTAA 
                   
               
               
                   
               
               
                 MspRecT 
                   E.   coli   
                 ATGGCCGAGAATGCCGTCACGAAACAGGATTCCCCTAAGGCA 
                 481 
               
               
                   
                   
                 CCGGAAACCATTAGTCAAGTGCTTCAGGTGCTGGTCCCACAA 
                   
               
               
                   
                   
                 TTGGCTCGTGCAGTACCTAAAGGCATGGATCCTGATCGTATT 
                   
               
               
                   
                   
                 GCACGTATCGTACAGACGGAGATTCGCAAATCCCGCAACGCA 
                   
               
               
                   
                   
                 AAAGCCGCTGGAATCGCAAAGCAAAGTTTAGACGATTGCACA 
                   
               
               
                   
                   
                 CAGGAGTCCTTTGCGGGAGCCTTACTGACCTCAGCGGCTTTA 
                   
               
               
                   
                   
                 GGGTTAGAGCCAGGCGTCAATGGGGAGTGTTATCTGGTACCC 
                   
               
               
                   
                   
                 TATCGTGATACACGCCGTGGTGTGGTCGAGTGCCAACTGATT 
                   
               
               
                   
                   
                 ATTGGATATCAAGGGATTGTCAAACTTTTTTGGCAACATCCGC 
                   
               
               
                   
                   
                 GCGCGAGCCGCATCGATGCGCAATGGGTTGGCGCGAACGAC 
                   
               
               
                   
                   
                 GAGTTCCATTATACGATGGGACTTAATCCTACCTTGAAACAT 
                   
               
               
                   
                   
                 GTAAAGGCAAAAGGTGATCGTGGAAACCCGGTCTACTTTTAC 
                   
               
               
                   
                   
                 GCCATCGTCGAGGTGACCGGTGCTGAGCCCTTATGGGATGTT 
                   
               
               
                   
                   
                 TTTACTGCTGATGAAATTCGTGAACTTCGTCGTGGCAAGGTTG 
                   
               
               
                   
                   
                 GATCGTCTGGAGATATTAAGGACCCCCAGCGTTGGATGGAAC 
                   
               
               
                   
                   
                 GCAAGACAGCATTGAAACAGGTACTGAAATTGGCTCCCAAAA 
                   
               
               
                   
                   
                 CCACACGCCTGGATGCGGCGATCCGCGCTGATGATCGTCCAG 
                   
               
               
                   
                   
                 GGACTGACCTTTCACAGTCGCAGGCTCTGGCCTTACCGTCTAC 
                   
               
               
                   
                   
                 CGTTAAGCCTACCGCAGACTATATTGATGGGGAGATCGCCGA 
                   
               
               
                   
                   
                 ACCGCATGAAGTCGATACACCACCAAAGAGTTCACGTGCTCA 
                   
               
               
                   
                   
                 ACGCGCACAACGTGCCACGGCACCGGCTCCTGATGTGCAAAT 
                   
               
               
                   
                   
                 GGCCAACCCCGACCAATTGAAGCGTCTGGGGGAGATCCAAA 
                   
               
               
                   
                   
                 AGGCGGAGAAGTACAATGATGCCGACTGGTTCAAGTTTTTGG 
                   
               
               
                   
                   
                 CGGACTCCGCCGGTGTGAAAGCGACGCGTGCTGCTGATCTTA 
                   
               
               
                   
                   
                 CGTTTGATGAAGCAAAGGCTGTAATCGACATGTTTGATGGGC 
                   
               
               
                   
                   
                 CAAACGCGTGA 
                   
               
               
                   
               
               
                 LrpRecT 
                   E.   coli   
                 ATGGCGAATCAAGTTGCACAGCAACAAAAACCGACAAAATT 
                 482 
               
               
                   
                   
                 AACCGATCTGGTTTTGGATAGAGTCAAGCAGATGCAAGACAC 
                   
               
               
                   
                   
                 CCAGGACCTTAGCCTTCCGAAAAACTATAACGCATCCAATGC 
                   
               
               
                   
                   
                 ACTGAATGCCGCGTTTTTAGAATTGCAGAAGGTACAAGACCG 
                   
               
               
                   
                   
                 GAACCACAGACCAGCACTGGAAGTCTGCTCGCACGATTCTAT 
                   
               
               
                   
                   
                 TGTAAAATCGCTGTTGGACATGACTTTGCAGGGCTTATCCCCT 
                   
               
               
                   
                   
                 GCGAAGGATCAGTGTTACTTCATAGTATATGGCAATGAGTTA 
                   
               
               
                   
                   
                 CAGATGCAGAGATCTTATTTCGGGACTGTCGCGGCAGTTAAA 
                   
               
               
                   
                   
                 AGATTAGATGGGGTGAAGAAGGTCCGGGCGGAAGTCGTGCA 
                   
               
               
                   
                   
                 TGAAAAGGACGACTTCGAAATTGGCGCCAATGAAGACATGG 
                   
               
               
                   
                   
                 AGCTGGTAGTGAAACGGTTTGTACCAAAGTTCGAAAATCAAG 
                   
               
               
                   
                   
                 ACAACCAAATAATAGGGGCGTTCGCAATGATTAAAACGGATG 
                   
               
               
                   
                   
                 AAGGTACGGACTTCACAGTTATGACGAAAAAGGAAATAGAT 
                   
               
               
                   
                   
                 CAAAGTTGGGCGCAAACACGCCAGAAGAACAATAAGGTACA 
                   
               
               
                   
                   
                 GCAGAACTTTAGTCAAGAAATGGCGAAACGTACAGTCCTTAA 
                   
               
               
                   
                   
                 TCGTGCCGCTAAGATGTTTATAAACACTTCAGACGATTCGGA 
                   
               
               
                   
                   
                 CTTATTAACCGGGGCCATAAATGACACGACCTCAAACGAGTA 
                   
               
               
                   
                   
                 TGACGATGAAAGAAGAGATGTGACACCAGTCGAGGACGAAA 
                   
               
               
                   
                   
                 AACAGAGCACGGATAAATTACTGGAGGGGTTTCAGAAGTCGC 
                   
               
               
                   
                   
                 AGGAGGCGAAAGCAAAAGGGGTTAGTAACGACGGAAACAGT 
                   
               
               
                   
                   
                 AATGAGGGAAAAGAGACAAGCGAGGAGGTGGCCGATGGACA 
                   
               
               
                   
                   
                 GACGGAACTGTTCTCTGAAGGTACTATTAAACCAGCAGATGA 
                   
               
               
                   
                   
                 AGCGGATAGCTAA 
                   
               
               
                   
               
               
                 MspRecT 
                 
                   M. 
                 
                 ATGGCAGAAAACGCTGTAACCAAGCAAGACAGTCCCAAAGC 
                 483 
               
               
                   
                 
                   smegmatis 
                 
                 GCCCGAGACCATATCGCAGGTATTGCAAGTGTTAGTGCCTCA 
                   
               
               
                   
                   
                 ATTAGCAAGAGCAGTCCCCAAAGGGATGGATCCTGACAGAAT 
                   
               
               
                   
                   
                 AGCACGCATAGTGCAGACCGAAATACGTAAGTCCCGTAATGC 
                   
               
               
                   
                   
                 CAAAGCTGCCGGCATCGCAAAACAATCGTTAGATGATTGTAC 
                   
               
               
                   
                   
                 CCAGGAGAGTTTTGCCGGGGCGCTGCTTACCTCAGCAGCCTT 
                   
               
               
                   
                   
                 AGGTCTGGAACCAGGAGTTAACGGAGAGTGTTATTTGGTCCC 
                   
               
               
                   
                   
                 ATACCGGGATACTCGTCGCGGAGTTGTTGAGTGCCAACTTAT 
                   
               
               
                   
                   
                 TATCGGTTACCAGGGAATAGTGAAGTTGTTCTGGCAACACCC 
                   
               
               
                   
                   
                 TCGTGCGTCCCGGATTGACGCGCAGTGGGTAGGTGCAAACGA 
                   
               
               
                   
                   
                 CGAATTCCACTACACTATGGGCCTTAATCCGACACTTAAACA 
                   
               
               
                   
                   
                 CGTCAAAGCGAAAGGGGATAGAGGAAACCCGGTGTACTTTTA 
                   
               
               
                   
                   
                 TGCAATTGTTGAGGTTACTGGAGCAGAGCCGTTATGGGATGT 
                   
               
               
                   
                   
                 CTTTACTGCCGATGAGATACGCGAGCTGCGTCGTGGCAAGGT 
                   
               
               
                   
                   
                 CGGGAGTTCAGGGGACATTAAAGATCCCCAACGGTGGATGG 
                   
               
               
                   
                   
                 AGCGGAAGACTGCGCTGAAACAGGTGTTGAAGTTGGCCCCCA 
                   
               
               
                   
                   
                 AAACGACCCGCCTTGACGCTGCAATCCGGGCGGATGATCGTC 
                   
               
               
                   
                   
                 CTGGGACTGACCTGTCCCAAAGCCAAGCCTTAGCCCTTCCAA 
                   
               
               
                   
                   
                 GTACTGTCAAGCCAACCGCAGATTACATTGACGGGGAAATCG 
                   
               
               
                   
                   
                 CAGAACCGCACGAAGTTGACACTCCTCCGAAGAGTAGCCGCG 
                   
               
               
                   
                   
                 CACAACGTGCCCAGCGCGCGACGGCACCAGCGCCGGATGTGC 
                   
               
               
                   
                   
                 AGATGGCAAATCCTGACCAACTTAAAAGACTGGGAGAGATA 
                   
               
               
                   
                   
                 CAGAAAGCAGAGAAGTACAACGACGCAGATTGGTTTAAGTTT 
                   
               
               
                   
                   
                 TTGGCGGACAGCGCTGGCGTCAAAGCAACTCGTGCGGCCGAC 
                   
               
               
                   
                   
                 TTGACCTTTGACGAAGCGAAGGCGGTCATAGATATGTTTGAT 
                   
               
               
                   
                   
                 GGTCCAAACGCCTGA 
                   
               
               
                   
               
               
                 PapRecT 
                   M.   smegmatis   
                 ATGGGCACCGCCCTGACCCCACTCTTGACCAAGTTTGCCACG 
                 484 
               
               
                   
                   
                 CGGTATGAGATGGGCACCACCCCAGAGGAAGTGGCGAACAC 
                   
               
               
                   
                   
                 CCTCAAGCAGACCTGCTTTAAGGGTCAGGTCAATGATAGCCA 
                   
               
               
                   
                   
                 GATGGTGGCCTTGCTGATCGTCGCGGACCAATATAAACTGAA 
                   
               
               
                   
                   
                 TCCATTTACCAAGGAACTCTATGCGTTTCCGGATAAGAACAA 
                   
               
               
                   
                   
                 TGGTATTGTCCCCGTCGTCGGCGTGGACGGTTGGGCGCGGAT 
                   
               
               
                   
                   
                 CATTAACGAGAACCCCCAATTCGATGGCATGGAATTTTCGAT 
                   
               
               
                   
                   
                 GGACCAGCAAGGGACCGAATGCACCTGCAAAATCTACCGGA 
                   
               
               
                   
                   
                 AAGACCGTAGCCATGCCATTAGCGCCACGGAGTATATGGCCG 
                   
               
               
                   
                   
                 AATGTAAACGTAATACGCAGCCATGGCAATCCCATCCACGCC 
                   
               
               
                   
                   
                 GGATGTTGCGCCACAAGGCGATGATCCAGTGTGCGCGGTTGG 
                   
               
               
                   
                   
                 CCTTTGGTTTCGCGGGCATCTATGACCAGGACGAAGCGGAAC 
                   
               
               
                   
                   
                 GCATCGTCGAGCGGGATGTGACCCCGGCCGAACAGTATGAGG 
                   
               
               
                   
                   
                 ACGTGTCGGAGGCGATTTGTCTCATCAAAGATAGCCCAACGA 
                   
               
               
                   
                   
                 TGGAGGATTTGCAGGCCGCCTTCAGCAACGCCTGGAAGGCGT 
                   
               
               
                   
                   
                 ACAAGACCAAAGGTGCCCGTGACCAACTGACGGCCGCGAAG 
                   
               
               
                   
                   
                 GACCAGCGTAAGAAAGAACTGTTGGATGCCCCAATTGATGTC 
                   
               
               
                   
                   
                 GAATTTGAGGAAACCGGGGACGATCGGGCGGCGTAA 
                   
               
               
                   
               
               
                 λ Beta 
                   C.   crescentus   
                 ATGAGCACGGCGCTCGCGACGCTCGCGGGGAAGCTGGCCGA 
                 485 
               
               
                   
                   
                 GCGTGTGGGCATGGATTCGGTCGATCCGCAGGAGCTCATCAC 
                   
               
               
                   
                   
                 CACGCTCCGGCAGACGGCCTTTAAGTGTGACGCGAGCGATGC 
                   
               
               
                   
                   
                 CCAGTTTATCGCCCTCCTGATCGTGGCCAATCAGTACGGCCTG 
                   
               
               
                   
                   
                 AACCCGTGGACGAAGGAAATCTACGCCTTTCCCGACAAGCAA 
                   
               
               
                   
                   
                 AACGGGATCGTGCCGGTGGTCGGCGTCGATGGGTGGTCCCGT 
                   
               
               
                   
                   
                 ATCATCAATGAAAATCAGCAATTTGATGGCATGGATTTCGAG 
                   
               
               
                   
                   
                 CAAGACAATGAATCCTGCACGTGCCGCATCTATCGGAAGGAC 
                   
               
               
                   
                   
                 CGCAACCATCCGATCTGCGTGACGGAATGGATGGATGAGTGC 
                   
               
               
                   
                   
                 CGCCGGGAGCCCTTTAAGACGCGGGAGGGCCGGGAAATCAC 
                   
               
               
                   
                   
                 CGGGCCCTGGCAGTCGCACCCCAAGCGGATGCTCCGTCATAA 
                   
               
               
                   
                   
                 GGCGATGATCCAATGTGCCCGCCTCGCCTTCGGGTTCGCGGG 
                   
               
               
                   
                   
                 CATCTACGACAAGGATGAAGCCGAGCGCATCGTGGAAAATA 
                   
               
               
                   
                   
                 CGGCCTACACGGCGGAGCGTCAGCCGGAACGGGATATCACG 
                   
               
               
                   
                   
                 CCGGTCAATGACGAAACGATGCAGGAAATCAATACCCTGCTCA 
                   
               
               
                   
                   
                 TCGCGCTCGACAAGACCTGGGACGATGATCTGCTGCCCCTGTG 
                   
               
               
                   
                   
                 TAGCCAAATCTTCCGTCGTGATATCCGCGCCTCGTCCGAACTG 
                   
               
               
                   
                   
                 ACCCAAGCGGAGGCGGTGAAGGCCCTGGGGTTCCTGAAGCA 
                   
               
               
                   
                   
                 GAAGGCCACCGAGCAAAAGGTCGCGGCCTAA 
                   
               
               
                   
               
               
                 PapRecT 
                   C.   crescentus   
                 ATGGGCACGGCGCTCACGCCGCTGCTCACCAAGTTTGCCACC 
                 486 
               
               
                   
                   
                 CGTTACGAGATGGGGACCACCCCCGAAGAAGTGGCGAACAC 
                   
               
               
                   
                   
                 CCTGAAGCAAACGTGCTTCAAGGGCCAGGTCAACGACTCGCA 
                   
               
               
                   
                   
                 GATGGTGGCCCTGCTCATCGTGGCCGATCAGTATAAGCTCAA 
                   
               
               
                   
                   
                 TCCGTTCACCAAGGAACTCTACGCGTTTCCCGATAAGAACAA 
                   
               
               
                   
                   
                 TGGGATCGTGCCGGTCGTCGGCGTCGACGGCTGGGCGCGTAT 
                   
               
               
                   
                   
                 CATCAATGAAAATCCGCAGTTCGACGGCATGGAATTCTCGAT 
                   
               
               
                   
                   
                 GGACCAACAAGGGACCGAATGTACGTGCAAGATCTATCGTAA 
                   
               
               
                   
                   
                 GGATCGTTCGCACGCGATCAGCGCCACGGAATACATGGCGGAGT 
                   
               
               
                   
                   
                 GTAAGCGGAATACGCAGCCGTGGCAATCCCACCCCCGCCGTA 
                   
               
               
                   
                   
                 TGCTGCGCCATAAGGCGATGATCCAATGTGCCCGCCTGGCGT 
                   
               
               
                   
                   
                 TTGGGTTCGCCGGCATCTACGATCAAGATGAAGCGGAGCGGA 
                   
               
               
                   
                   
                 TCGTCGAACGCGATGTGACGCCCGCCGAACAATATGAAGACG 
                   
               
               
                   
                   
                 TGTCGGAAGCGATCTGCCTGATCAAGGACAGCCCCACGATGG 
                   
               
               
                   
                   
                 AAGATCTCCAAGCGGCCTTTAGCAATGCCTGGAAGGCCTACA 
                   
               
               
                   
                   
                 AGACGAAGGGGGCGCGTGACCAACTGACGGCGGCCAAGGAT 
                   
               
               
                   
                   
                 CAACGGAAGAAGGAGCTGCTGGATGCGCCGATCGATGTCGA 
                   
               
               
                   
                   
                 ATTCGAGGAAACGGGGGACGATCGTGCCGCGTAA 
                   
               
               
                   
               
               
                 EcSSB 
                   L.   lactis   
                 ATGGCAAGCCGTGGGGTTAACAAAGTTATTCTTGTTGGAAACTTA 
                 487 
               
               
                   
                   
                 GGACAAGACCCGGAAGTGCGTTATATGCCTAATGGAGGCGCG 
                   
               
               
                   
                   
                 GTAGCCAATATCACCTTGGCCACAAGCGAGTCTTGGCGAGAC 
                   
               
               
                   
                   
                 AAAGCAACAGGTGAAATGAAAGAACAAACTGAATGGCACAG 
                   
               
               
                   
                   
                 AGTAGTTTTGTTTGGAAAATTGGCAGAGGTAGCCTCAGAATA 
                   
               
               
                   
                   
                 CTTGCGAAAGGGCAGTCAGGTCTATATAGAGGGCCAATTGCG 
                   
               
               
                   
                   
                 TACCCGTAAGTGGACAGACCAGAGCGGACAAGATCGTTATAC 
                   
               
               
                   
                   
                 GACCGAGGTCGTTGTTAATGTAGGAGGCACAATGCAGATGTT 
                   
               
               
                   
                   
                 GGGGGGGAGACAGGGCGGAGGCGCTCCGGCTGGAGGCAATAT 
                   
               
               
                   
                   
                 CGGGGGTGGCCAACCTCAAGGTGGGTGGGGGCAGCCACAGCAA 
                   
               
               
                   
                   
                 CCGCAAGGAGGTAATCAATTTAGTGGAGGAGCCCAATCACGT 
                   
               
               
                   
                   
                 CCGCAGCAGTCTGCGCCTGCCGCCCCTTCTAATGAACCGCCG 
                   
               
               
                   
                   
                 ATGGATTTTGACGATGATATACCTTTCTGA 
                   
               
               
                   
               
               
                 PaSSB 
                   L.   lactis   
                 ATGGCCCGTGGAGTGAACAAAGTAATTCTTGTCGGTAATGTG 
                 488 
               
               
                   
                   
                 GGTGGGGATCCAGAGACGCGATACATGCCAAACGGGAACGCCG 
                   
               
               
                   
                   
                 TGACAAATATCACCTTAGCCACGAGCGAATCTTGGAAGGACA 
                   
               
               
                   
                   
                 AACAAACAGGTCAGCAACAAGAACGAACCGAATGGCATAGA 
                   
               
               
                   
                   
                 GTTGTATTTTTTGGCCGACTTGCTGAGATCGCGGGTGAGTACC 
                   
               
               
                   
                   
                 TTAGAAAGGGTTCTCAGGTTTATGTCGAGGGCTCATTAAGAA 
                   
               
               
                   
                   
                 CACGTAAGTGGCAGGGGCAGGACGGGCAAGACCGATATACA 
                   
               
               
                   
                   
                 ACTGAAATAGTAGTGGACATAAACGGCAACATGCAACTTCTT 
                   
               
               
                   
                   
                 GGTGGCAGACCGAGTGGGGACGATTCACAGAGAGCTCCAAG 
                   
               
               
                   
                   
                 AGAACCTATGCAGCGACCACAGCAGGCTCCTCAACAGCAGTC 
                   
               
               
                   
                   
                 TCGTCCGGCCCCTCAGCAGCAACCGGCTCCGCAACCTGCACAAG 
                   
               
               
                   
                   
                 ATTACGATAGTTTTGATGATGATATTCCATTCTAA 
                   
               
               
                   
               
               
                 MsSSB 
                   L.   lactis   
                 ATGGCGGGAGACACAACAATTACGGTTGTGGGAAACTTGACA 
                 489 
               
               
                   
                   
                 GCCGATCCTGAATTGCGATTCACCCCATCAGGCGCTGCGGTG 
                   
               
               
                   
                   
                 GCGAATTTCACAGTCGCGAGCACCCCACGAATGTTTGATAGA 
                   
               
               
                   
                   
                 CAATCAGGCGAATGGAAGGATGGCGAAGCGTTGTTTTTACGA 
                   
               
               
                   
                   
                 TGCAACATCTGGAGAGAGGCGGCCGAGAACGTCGCCGAAAG 
                   
               
               
                   
                   
                 CCTTACCCGTGGCAGTCGAGTGATTGTAACCGGACGATTAAA 
                   
               
               
                   
                   
                 GCAAAGAAGTTTTGAGACGAGAGAAGGAGAGAAACGAACTGT 
                   
               
               
                   
                   
                 GGTAGAGGTAGAGGTGGATGAAATAGGTCCTAGTTTGCGTTAT 
                   
               
               
                   
                   
                 GCCACAGCGAAAGTAAACAAAGCCTCTCGTAGTGGTGGCGG 
                   
               
               
                   
                   
                 GGGGGGCGGCTTTGGTTCAGGGGGTGGAGGTTCACGACAGA 
                   
               
               
                   
                   
                 GCGAGCCAAAGGATGATCCTTGGGGCAGTGCCCCTGCATCAG 
                   
               
               
                   
                   
                 GCAGTTTTAGCGGAGCAGATGACGAGCCGCCTTTTTGA 
                   
               
               
                   
               
               
                 LrSSB 
                   L.   lactis   
                 ATGCTTAATCGTGCAGTCTTAACTGGGCGTTTAACAAGAGAT 
                 490 
               
               
                   
                   
                 CCCGAGTTGCGGTACACAACCAGCGGGACAGCAGTTGTTTCA 
                   
               
               
                   
                   
                 TTTACGTTAGCTGTTGATCGGCAATTCCGAAACCAAAATGGT 
                   
               
               
                   
                   
                 GATCGTGATGCTGATTTTATCAATTGCGTTATTTGGCGTAAAT 
                   
               
               
                   
                   
                 CCGCTGAAAACTTTAGTAACTTTACGCATAAGGGTTCACTTGT 
                   
               
               
                   
                   
                 TGGAATTGAAGGGCGTATTCAAACCCGGAATTATGAAAACCA 
                   
               
               
                   
                   
                 ACAGGGTAACCGTGTGTATGTTACCGAAGTTGTTGTAGATAA 
                   
               
               
                   
                   
                 TTTTGCATTGTTAGAACCTCGTCAAAATGGTGGCATGAACCA 
                   
               
               
                   
                   
                 ATCAGGGGTTCAACAACCATTTAACAGCAACCAACAATCATT 
                   
               
               
                   
                   
                 TGGTGCTCAGGCTCCACAATATGGTAGTCAACCACAACCTGG 
                   
               
               
                   
                   
                 AAATAATGCTCCTCAAAGTAATCCGTCACCAAGTATGGATAATG 
                   
               
               
                   
                   
                 GTTTCGATCCCAATCAAAATGCTGGCAACCAGTTCCCTGGAAGC 
                   
               
               
                   
                   
                 AGTGATGATGGTGGTCAATCCATTGATTTAGCTGATGACGAATTA 
                   
               
               
                   
                   
                 CCATTCTAA 
                   
               
               
                   
               
               
                 LlSSB 
                   L.   lactis   
                 ATGATTAACAATGTTGTATTAGTGGGACGCATTACTCGCGAT 
                 491 
               
               
                   
                   
                 CCTGAACTTCGTTACACCCCTCAAAATCAAGCTGTTGCAACTTTT 
                   
               
               
                   
                   
                 TCATTGGCTGTAAATCGTCAATTTAAAAATGCTAACGGTGAAC 
                   
               
               
                   
                   
                 GTGAGGCTGATTTCATTAACTGCGTTATTTGGCGCCAACAAG 
                   
               
               
                   
                   
                 CTGAAAATTTGGCAAATTGGGCTAAAAAAGGAGCTTTGATCG 
                   
               
               
                   
                   
                 GTGTAACTGGTCGAATTCAAACACGTAATTATGAAAATCAAC 
                   
               
               
                   
                   
                 AAGGTCAACGCGTTTATGTGACTGAGGTTGTGGCTGATAGTT 
                   
               
               
                   
                   
                 TCCAAATGTTGGAAAGTAGATCTGCTCGCGATGGTATGGGAG 
                   
               
               
                   
                   
                 GCGGAGCTTCTGCCGGTTCATATTCTGCACCAAGCCAATCTAC 
                   
               
               
                   
                   
                 AAATAATACTCCACGTCCACAAACGAATAATAATAGTGCAAC 
                   
               
               
                   
                   
                 ACCGAATTTCGGTCGTGATGCTGACCCATTTGGTAGCTCACCT 
                   
               
               
                   
                   
                 ATGGAAATCTCGGATGATGATCTTCCATTCTAA 
                   
               
               
                   
               
               
                 PapSSB 
                   L.   lactis   
                 ATGCGTGGGGTTAATAAGGTAATCTTAGTTGGTAACGTGGGT 
                 492 
               
               
                   
                   
                 GGGGACCCGGAGACCCGATATATGCCAAATGGAAACGCGGT 
                   
               
               
                   
                   
                 AACAAACATCACCCTTGCAACTAGTGAGAGTTGGAAAGATAA 
                   
               
               
                   
                   
                 ACAAACTGGCCAACAGCAAGAACGTACTGAATGGCACAGAGTG 
                   
               
               
                   
                   
                 GTGTTTTTTGGCAAATTAGCCGAAATTGTCGGCCAACACGTTAA 
                   
               
               
                   
                   
                 GAAAGGCCAGCAGCTTTACGTCGAAGGGTCATTGCGAACCCG 
                   
               
               
                   
                   
                 TAAGTGGCAAGCGCAGGATGGTCAGGACAGATATACGACAG 
                   
               
               
                   
                   
                 AAATCATAGTAGATATGCACGGACAAATGCAAATGTTCGGGG 
                   
               
               
                   
                   
                 GAAAACCTGGGAATGAGCAGGCCGCACAGTCAAGATCATCT 
                   
               
               
                   
                   
                 ACCCAACAACAAAGCGCCCCGCAACAACGATCAGCACAGGA 
                   
               
               
                   
                   
                 TGAATTTGATGATGATATACCTTTATAA 
                   
               
               
                   
               
               
                 EcSSB 
                   E.   coli   
                 ATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAAT 
                 493 
               
               
                   
                   
                 CTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGC 
                   
               
               
                   
                   
                 GCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGA 
                   
               
               
                   
                   
                 TAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCG 
                   
               
               
                   
                   
                 CGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATA 
                   
               
               
                   
                   
                 TCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGT 
                   
               
               
                   
                   
                 ACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTACACC 
                   
               
               
                   
                   
                 ACAGAAGTCGTGGTGAACGTTGGCGGCACCATGCAGATGCTG 
                   
               
               
                   
                   
                 GGTGGTCGTCAGGGTGGTGGCGCTCCGGCAGGTGGCAATATC 
                   
               
               
                   
                   
                 GGTGGTGGTCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAG 
                   
               
               
                   
                   
                 CCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGC 
                   
               
               
                   
                   
                 CCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCG 
                   
               
               
                   
                   
                 ATGGACTTTGATGATGACATTCCGTTCTGA 
                   
               
               
                   
               
               
                 PaSSB 
                   E.   coli   
                 ATGGCTCGCGGGGTAAATAAGGTCATTTTGGTTGGCAATGTT 
                 494 
               
               
                   
                   
                 GGTGGTGATCCCGAGACACGCTATATGCCTAACGGGAACGCC 
                   
               
               
                   
                   
                 GTCACTAATATCACACTGGCAACGTCCGAGTCATGGAAGGAT 
                   
               
               
                   
                   
                 AAACAGACAGGTCAACAGCAAGAACGCACGGAGTGGCACCG 
                   
               
               
                   
                   
                 CGTGGTATTTTTCGGGCGTCTTGCTGAGATCGCCGGAGAGTAT 
                   
               
               
                   
                   
                 TTACGCAAAGGATCGCAGGTATACGTTGAGGGTTCTTTACGC 
                   
               
               
                   
                   
                 ACGCGCAAGTGGCAGGGTCAGGATGGTCAGGACCGTTATACT 
                   
               
               
                   
                   
                 ACCGAAATTGTAGTCGACATTAACGGGAACATGCAATTATTA 
                   
               
               
                   
                   
                 GGTGGTCGTCCGAGCGGAGATGACTCCCAGCGCGCCCCCCGC 
                   
               
               
                   
                   
                 GAGCCCATGCAGCGTCCGCAACAGGCTCCACAGCAGCAGAG 
                   
               
               
                   
                   
                 CCGCCCTGCCCCTCAACAACAACCCGCTCCTCAACCCGCGCA 
                   
               
               
                   
                   
                 AGATTACGATTCGTTTGACGACGATATTCCTTTTTAA 
                   
               
               
                   
               
               
                 MsSSB 
                   E.   coli   
                 ATGGCAGGGGATACCACGATAACCGTTGTCGGTAACTTAACC 
                 495 
               
               
                   
                   
                 GCGGACCCTGAACTTCGTTTCACACCATCCGGTGCAGCGGTT 
                   
               
               
                   
                   
                 GCAAACTTCACGGTCGCTTCTACGCCTCGTATGTTCGACAGAC 
                   
               
               
                   
                   
                 AGTCTGGTGAGTGGAAAGATGGGGAAGCACTGTTTTTAAGAT 
                   
               
               
                   
                   
                 GCAATATATGGCGCGAAGCAGCAGAGAATGTAGCCGAGAGTT 
                   
               
               
                   
                   
                 TAACCAGAGGTTCACGTGTGATCGTAACTGGCCGTTTGAAACA 
                   
               
               
                   
                   
                 ACGCTCCTTTGAAACACGCGAAGGCGAGAAACGCACGGTAGTT 
                   
               
               
                   
                   
                 GAGGTCGAAGTCGACGAGATAGGCCCGTCCTTACGCTATGCC 
                   
               
               
                   
                   
                 ACAGCGAAAGTCAACAAAGCGTCTCGCAGCGGAGGCGGTGG 
                   
               
               
                   
                   
                 GGGCGGGTTTGGTAGTGGTGGGGGGGGTAGTCGTCAATCGGA 
                   
               
               
                   
                   
                 ACCCAAGGATGACCCGTGGGGGTCGGCACCAGCTTCAGGAA 
                   
               
               
                   
                   
                 GTTTTTCTGGGGCCGATGACGAGCCGCCATTTTGA 
                   
               
               
                   
               
               
                 LrSSB 
                   E.   coli   
                 ATGCTGAACCGTGCCGTGCTTACTGGTCGCCTTACTCGTGACC 
                 496 
               
               
                   
                   
                 CTGAATTGCGCTATACGACATCAGGGACTGCAGTAGTGTCCT 
                   
               
               
                   
                   
                 TTACATTGGCGGTCGATCGTCAATTTCGTAACCAAAACGGCG 
                   
               
               
                   
                   
                 ACCGCGACGCCGATTTTATCAACTGTGTGATTTGGAGAAAGA 
                   
               
               
                   
                   
                 GCGCCGAGAACTTTAGCAATTTCACTCATAAAGGGAGTTTAG 
                   
               
               
                   
                   
                 TTGGAATCGAGGGGCGTATCCAAACGAGAAACTACGAAAACC 
                   
               
               
                   
                   
                 AGCAAGGCAATCGCGTCTACGTAACCGAAGTCGTAGTAGATA 
                   
               
               
                   
                   
                 ACTTCGCCCTGTTGGAACCACGGCAAAACGGTGGGATGAACC 
                   
               
               
                   
                   
                 AATCTGGAGTTCAACAACCCTTCAACAGTAACCAGCAGTCTT 
                   
               
               
                   
                   
                 TCGGGGCTCAGGCACCTCAATATGGCAGTCAGCCACAACCTG 
                   
               
               
                   
                   
                 GAAACAATGCCCCACAGTCTAACCCAAGTCCCTCTATGGACAAT 
                   
               
               
                   
                   
                 GGGTTTGACCCCAACCAGAATGCGGGGAACCAATTCCCTGGG 
                   
               
               
                   
                   
                 AGCTCGGATGACGGCGGCCAATCAATTGATCTGGCTGACGATGA 
                   
               
               
                   
                   
                 ATTACCCTTTTAA 
                   
               
               
                   
               
               
                 PaSSB 
                 
                   M. 
                 
                 ATGGCGCGTGGGGTGAACAAAGTCATCCTCGTGGGGAATGTC 
                 497 
               
               
                   
                 
                   smegmatis 
                 
                 GGTGGCGATCCCGAAACGCGTTATATGCCGAATGGGAATGCG 
                   
               
               
                   
                   
                 GTCACCAATATCACGCTCGCCACCAGCGAGTCCTGGAAAGAT 
                   
               
               
                   
                   
                 AAACAAACGGGTCAACAGCAGGAGCGTACGGAGTGGCATCG 
                   
               
               
                   
                   
                 GGTGGTCTTCTTCGGGCGCCTCGCCGAGATCGCCGGGGAATA 
                   
               
               
                   
                   
                 CCTCCGTAAAGGTTCGCAGGTCTATGTGGAGGGCTCGCTGCG 
                   
               
               
                   
                   
                 GACCCGTAAATGGCAAGGTCAGGATGGCCAGGATCGGTACA 
                   
               
               
                   
                   
                 CGACGGAAATCGTCGTGGACATTAACGGTAATATGCAATTGC 
                   
               
               
                   
                   
                 TCGGTGGCCGCCCCTCCGGCGATGATAGCCAGCGTGCCCCGC 
                   
               
               
                   
                   
                 GTGAACCGATGCAACGCCCGCAACAAGCGCCCCAACAGCAA 
                   
               
               
                   
                   
                 TCGCGGCCCGCGCCGCAGCAGCAGCCGGCCCCGCAACCAGCC 
                   
               
               
                   
                   
                 CAGGACTACGATTCGTTTGATGATGACATTCCATTTTAA 
                   
               
               
                   
               
               
                 PaSSB 
                 
                   C. 
                 
                 ATGGCGCGTGGGGTCAATAAGGTGATCCTGGTCGGCAACGTG 
                 498 
               
               
                   
                 
                   crescentus 
                 
                 GGGGGCGATCCCGAAACCCGGTACATGCCGAACGGCAACGC 
                   
               
               
                   
                   
                 GGTCACCAACATCACCCTGGCGACCAGCGAGAGCTGGAAGG 
                   
               
               
                   
                   
                 ATAAGCAAACGGGCCAGCAGCAAGAACGTACGGAATGGCAT 
                   
               
               
                   
                   
                 CGTGTGGTCTTTTTCGGCCGGCTGGCGGAGATCGCGGGGGAA 
                   
               
               
                   
                   
                 TACCTCCGTAAGGGGTCCCAAGTCTACGTGGAGGGCTCGCTG 
                   
               
               
                   
                   
                 CGGACCCGGAAGTGGCAAGGGCAAGATGGGCAAGATCGCTA 
                   
               
               
                   
                   
                 CACCACGGAGATCGTCGTCGACATCAACGGGAACATGCAGCT 
                   
               
               
                   
                   
                 CCTCGGGGGGCGTCCCTCGGGGGACGATTCCCAACGCGCCCC 
                   
               
               
                   
                   
                 CCGTGAGCCCATGCAACGCCCGCAGCAAGCGCCCCAGCAACA 
                   
               
               
                   
                   
                 ATCGCGTCCCGCCCCCCAGCAACAGCCGGCGCCCCAACCGGC 
                   
               
               
                   
                   
                 GCAGGACTACGACTCGTTCGACGATGATATCCCCTTTTAA 
                   
               
               
                   
               
               
                 PapExo 
                   L.   lactis   
                 ATGATAGAACAGCGTAGTGATGAATGGTTCGCGCAGCGACTT 
                 499 
               
               
                   
                   
                 GGCCGAGTCACCGCGAGTAAAGTAAAGGATGTCATGGCGAA 
                   
               
               
                   
                   
                 GGGGCGATCAGGTGCGCCATCAGCCACCAGACAGAATTACATG 
                   
               
               
                   
                   
                 ATGCAATTGTTATGTGAGAGACTTACCGGGAAACGAGAAGAGGG 
                   
               
               
                   
                   
                 GTTCACGAGTGCGGCGATGCAGCGTGGGACGGACCTTGAACC 
                   
               
               
                   
                   
                 AATAGCGCGATCAGCTTATGAGTTTAACGCAGGAGTAATGAC 
                   
               
               
                   
                   
                 TATAGAAACAGGCCTTATTATCCATCCACGTATCGACGGTTTCG 
                   
               
               
                   
                   
                 GAGCTAGTCCGGATGGGCTTGCGGGAGAGCATGGATTAGTGGA 
                   
               
               
                   
                   
                 AATTAAGTGCCCGTCAACAGCAACGCACATTTATACCATGCA 
                   
               
               
                   
                   
                 AAGTGGTAAGCACGACCCTCAGTACGAATGGCAAATGCTTGC 
                   
               
               
                   
                   
                 TCAAATGAGTTGCTCAGGCAGAGAGTGGGTGGATTTCGTGTCAT 
                   
               
               
                   
                   
                 TCGACGATAGATTGCCAGACGAATTGCAATATGTTTGTTTCCG 
                   
               
               
                   
                   
                 TTATCACCGTGATGAAGAGAGAATAAGAGAAATGGAAAGCG 
                   
               
               
                   
                   
                 AAGTTAAGGCATTCTTGGAGGAATTAGCTGAATTGGAACACC 
                   
               
               
                   
                   
                 AAATGCGTGAACGTATGAGAAAGGCGGCCTAA 
                   
               
               
                   
               
               
                 LrpExo 
                   L.   lactis   
                 ATGAAACTTACGGCCAACAATTACTATAGCCATGAGACTGAC 
                 500 
               
               
                   
                   
                 TGGCAATATATGTCAGTTTCATTGTTCAAAGACTTCGAAAAGT 
                   
               
               
                   
                   
                 GCGAAGCGCGTGCATTAGCAAAGTTGAAGGAAGATTGGCAA 
                   
               
               
                   
                   
                 CCTGTTTCTAGTCCAGTTCCGCTTTTGGTTGGGAACTATGTAC 
                   
               
               
                   
                   
                 ACAGTTATTTCGAAAGTGCTAAGAGCCACCAAGATTTTATAG 
                   
               
               
                   
                   
                 AGGCGAATAAGAAAGAGCTTATGACCAGACCTACTAAGACA 
                   
               
               
                   
                   
                 AACCCGAACGGCCATCTTAGAGCGGAATTTAAGGGGGCAAAC 
                   
               
               
                   
                   
                 TCAATGATTCAGACCTTGCAAGCCGACGATATGTTTAACTACT 
                   
               
               
                   
                   
                 TTTATGCACCAGGGGACAAAGAAGTTATCGTTACCGGAGAGA 
                   
               
               
                   
                   
                 TAGACGGCTATTTGTGGAAGGGAAAAATAGACTCTTTAGTTC 
                   
               
               
                   
                   
                 TTGACAAAGGCTATTTTTGCGATCTTAAGACGGTAGACGACA 
                   
               
               
                   
                   
                 TTCATAAGGGACATTGGAATACGTATGAACACAGATACGTCC 
                   
               
               
                   
                   
                 CGTTCATTCAAGACCGAGAATATGATTTACAAATGGCTGTTT 
                   
               
               
                   
                   
                 ATAGAGAGTTAATCAAGCAGACGTTCGGGAAAAAGTGCCAA 
                   
               
               
                   
                   
                 CCTTTAATTTTTGCCATCTCTAAGCAAACTCCGCCTGACAAGAT 
                   
               
               
                   
                   
                 GGCCATCGACTTTAATGGCGTTGATGACGACTATCAGATGCAG 
                   
               
               
                   
                   
                 GCCGATCTTGATAAGGTCAAAGAGCTTCAACCACACTTTTGG 
                   
               
               
                   
                   
                 AAAGTAATGACGGGAGAGGAAGAGCCTGTCCACTGTGGTAA 
                   
               
               
                   
                   
                 GTGCGACTATTGTAGAGAAACGAAAATGTTGAGCGGCTTCAT 
                   
               
               
                   
                   
                 CCACGCATCAGAAATAGAGGTTTAA 
                   
               
               
                   
               
               
                 mCardinal 
                   
                 ATGCACCATCATCACCACCACGGTTCCGGCATGGTTTCTAAA 
                 501 
               
               
                 RBS eGFP 
                   
                 GGTGAAGAACTGATCAAGGAAAACATGCACATGAAGCTGTA 
                   
               
               
                   
                   
                 TATGGAAGGTACCGTTAACAACCACCATTTCAAATGCACCAC 
                   
               
               
                   
                   
                 TGAAGGTGAAGGTAAACCGTACGAGGGTACGCAGACCCAAC 
                   
               
               
                   
                   
                 GTATTAAAGTTGTTGAGGGTGGTCCGCTGCCGTTCGCGTTCGA 
                   
               
               
                   
                   
                 CATCCTGGCGACCTGTTTCATGTACGGCTCTAAAACCTTCATC 
                   
               
               
                   
                   
                 AACCACACCCAGGGTATCCCTGACTTCTTCAAACAGTCTTTCC 
                   
               
               
                   
                   
                 CGGAGGGTTTCACCTGGGAACGTGTTACCACCTACGAAGACG 
                   
               
               
                   
                   
                 GTGGTGTACTGACCGTTACCCAGGACACTTCTCTGCAGGACG 
                   
               
               
                   
                   
                 GTTGCCTGATCTACAACGTTAAACTCCGCGGTGTTAATTTCCC 
                   
               
               
                   
                   
                 GTCTAACGGTCCGGTTATGCAAAAAAAGACGCTGGGTTGGGA 
                   
               
               
                   
                   
                 AGCGACTACGGAAACTCTCTACCCTGCCGATGGCGGCCTCGA 
                   
               
               
                   
                   
                 AGGTCGTTGTGATATGGCGCTGAAACTGGTTGGTGGCGGTCA 
                   
               
               
                   
                   
                 CCTGCACTGCAATCTGAAAACTACCTACCGTTCTAAAAAACC 
                   
               
               
                   
                   
                 AGCTAAAAACCTCAAAATGCCGGGTGTTTACTTTGTTGATCGT 
                   
               
               
                   
                   
                 CGTCTGGAACGTATCAAAGAAGCAGACAACGAAACTTACGTT 
                   
               
               
                   
                   
                 GAACAGCACGAAGTTGCGGTGGCGCGTTACTGCGACCTGCCA 
                   
               
               
                   
                   
                 TCTAAACTGGGTCACAAAGGTATGGACGAACTGTACAAATAA 
                   
               
               
                   
                   
                 AAAAAATAGGAGGAAAAACATATGGGTTCTCACCACCATCAC 
                   
               
               
                   
                   
                 CACCACAGCGGCTCTAAAGGTGAAGAATTATTCACTGGTGTT 
                   
               
               
                   
                   
                 GTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACA 
                   
               
               
                   
                   
                 AATTTTCTGTCTCCGGTGAAGGTGAAGGTGATGCTACGTACG 
                   
               
               
                   
                   
                 GTAAATTGACCTTAAAATTTATTTGTACTACTGGTAAATTGCC 
                   
               
               
                   
                   
                 AGTTCCATGGCCAACCTTAGTCACTACTTTCACTTATGGTGTT 
                   
               
               
                   
                   
                 CAATGTTTTTCTAGATACCCAGATCATATGAAACAACATGAC 
                   
               
               
                   
                   
                 TTTTTCAAGTCTGCCATGCCAGAAGGTTATGTTCAAGAAAGA 
                   
               
               
                   
                   
                 ACTATTTTTTTCAAAGATGACGGTAACTACAAGACCAGAGCT 
                   
               
               
                   
                   
                 GAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAA 
                   
               
               
                   
                   
                 TTAAAAGGTATTGATTTTAAAGAAGATGGTAACATTTTAGGT 
                   
               
               
                   
                   
                 CACAAATTGGAATACAACTATAACTCTCACAATGTTTACATC 
                   
               
               
                   
                   
                 ATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAA 
                   
               
               
                   
                   
                 ATTAGACACAACATTGAAGATGGTTCTGTTCAATTAGCTGAC 
                   
               
               
                   
                   
                 CATTATCAACAAAATACTCCAATTGGTGATGGTCCAGTCTTGT 
                   
               
               
                   
                   
                 TACCAGACAACCATTACTTATCCACTCAATCTGCCTTATCCAA 
                   
               
               
                   
                   
                 AGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATT 
                   
               
               
                   
                   
                 TGTTACTGCTGCTGGTATTACCCATGGTATGGATGAATTGTAC 
                   
               
               
                   
                   
                 AAATAA 
                   
               
               
                   
               
               
                 E.  coli  MutL 
                   L.   lactis   
                 ATGCCTATACAAGTGTTGCCTCCACAGTTGGCCAACCAAATC 
                 502 
               
               
                 E32K 
                   
                 GCGGCAGGCGAGGTGGTCGAACGTCCGGCTTCAGTCGTTAAG 
                   
               
               
                   
                   
                 GAATTGGTAAAAAATTCTTTGGATGCAGGGGCAACGAGAATT 
                   
               
               
                   
                   
                 GATATTGACATCGAACGAGGCGGGGCCAAGTTAATCAGAATC 
                   
               
               
                   
                   
                 CGAGACAATGGGTGTGGGATTAAAAAGGATGAACTTGCTTTG 
                   
               
               
                   
                   
                 GCGTTGGCACGTCACGCGACCAGCAAAATAGCGTCTCTTGAC 
                   
               
               
                   
                   
                 GACTTGGAAGCTATTATCAGTCTTGGTTTCCGTGGGGAAGCCT 
                   
               
               
                   
                   
                 TAGCATCTATTAGCTCTGTGTCACGTTTGACTTTGACTAGCAG 
                   
               
               
                   
                   
                 AACGGCGGAACAGCAGGAAGCATGGCAAGCGTATGCGGAAG 
                   
               
               
                   
                   
                 GACGAGACATGAACGTCACGGTTAAGCCGGCAGCCCACCCG 
                   
               
               
                   
                   
                 GTCGGCACGACCTTGGAGGTCTTGGACTTGTTCTATAATACCC 
                   
               
               
                   
                   
                 CTGCACGTCGTAAATTCTTACGAACCGAAAAGACCGAATTTA 
                   
               
               
                   
                   
                 ACCATATAGATGAGATAATAAGAAGAATTGCGTTAGCACGTT 
                   
               
               
                   
                   
                 TCGATGTTACTATAAATTTGAGTCATAACGGAAAAATCGTTA 
                   
               
               
                   
                   
                 GACAGTATCGAGCCGTGCCTGAGGGCGGGCAGAAGGAAAGA 
                   
               
               
                   
                   
                 AGATTAGGGGCTATTTGTGGCACTGCTTTTCTTGAACAAGCAC 
                   
               
               
                   
                   
                 TTGCGATCGAATGGCAACATGGGGACCTTACCTTGCGAGGTT 
                   
               
               
                   
                   
                 GGGTAGCGGACCCGAATCATACAACACCAGCGTTGGCAGAG 
                   
               
               
                   
                   
                 ATACAATATTGCTATGTAAACGGACGAATGATGAGAGATCGT 
                   
               
               
                   
                   
                 TTGATCAACCACGCAATACGACAGGCTTGCGAAGATAAGTTG 
                   
               
               
                   
                   
                 GGGGCGGATCAACAGCCAGCTTTCGTCCTTTATCTTGAAATTG 
                   
               
               
                   
                   
                 ACCCTCATCAGGTAGATGTGAATGTACATCCGGCCAAACACG 
                   
               
               
                   
                   
                 AGGTTCGTTTTCATCAAAGTCGACTTGTGCATGATTTTATATA 
                   
               
               
                   
                   
                 CCAGGGTGTCTTAAGTGTCTTGCAGCAGCAGCTTGAGACACC 
                   
               
               
                   
                   
                 TTTACCTTTAGATGATGAGCCGCAGCCAGCTCCGCGTAGTATC 
                   
               
               
                   
                   
                 CCTGAGAATCGAGTTGCCGCCGGCAGAAATCATTTCGCAGAA 
                   
               
               
                   
                   
                 CCGGCAGCCCGTGAACCTGTAGCACCGAGATACACCCCGGCT 
                   
               
               
                   
                   
                 CCTGCCTCTGGATCACGTCCTGCTGCCCCGTGGCCTAACGCAC 
                   
               
               
                   
                   
                 AACCGGGCTATCAGAAGCAGCAGGGTGAAGTTTATCGTCAAT 
                   
               
               
                   
                   
                 TGTTACAAACTCCGGCACCAATGCAAAAACTTAAGGCCCCGG 
                   
               
               
                   
                   
                 AGCCGCAGGAACCGGCGCTTGCTGCAAATTCACAATCTTTCG 
                   
               
               
                   
                   
                 GACGAGTTTTAACAATAGTGCATAGTGACTGCGCATTACTTG 
                   
               
               
                   
                   
                 AGCGTGACGGCAACATTAGTTTGCTTTCATTGCCTGTTGCCGA 
                   
               
               
                   
                   
                 GCGTTGGTTGAGACAAGCACAATTAACCCCTGGTGAAGCACC 
                   
               
               
                   
                   
                 AGTCTGTGCACAGCCATTATTGATCCCATTGCGTTTAAAGGTC 
                   
               
               
                   
                   
                 TCAGCCGAGGAAAAGAGTGCTTTGGAAAAAGCCCAAAGTGC 
                   
               
               
                   
                   
                 CCTTGCAGAGCTTGGAATTGATTTCCAAAGCGACGCACAACA 
                   
               
               
                   
                   
                 CGTTACGATAAGAGCGGTTCCATTACCGTTAAGACAGCAAAA 
                   
               
               
                   
                   
                 CTTACAAATTCTTATACCAGAGCTTATCGGGTATTTGGCGAAA 
                   
               
               
                   
                   
                 CAGAGCGTATTCGAACCAGGTAATATCGCCCAGTGGATAGCG 
                   
               
               
                   
                   
                 CGTAACCTTATGTCAGAACACGCGCAGTGGAGTATGGCGCAA 
                   
               
               
                   
                   
                 GCTATCACATTGTTAGCCGACGTTGAGCGTTTGTGCCCACAGT 
                   
               
               
                   
                   
                 TGGTGAAAACGCCTCCGGGTGGACTTCTTCAAAGTGTGGACT 
                   
               
               
                   
                   
                 TACATCCAGCAATTAAGGCTCTTAAAGATGAATAA 
                   
               
               
                   
               
               
                   L.   lactis  MutL 
                   L.   lactis   
                 GTGGGAAAAATTATTGAACTAAATGAAGCGCTCGCCAATCAA 
                 503 
               
               
                 E33K 
                   
                 ATTGCTGCTGGAGAGGTGGTTGAGCGGCCTGCTAGTGTTGTC 
                   
               
               
                   
                   
                 AAAGAATTAGTCAAAAACTCAATTGATGCTGGAAGCAGTAAA 
                   
               
               
                   
                   
                 ATTATTATCAATGTTGAAGAAGCAGGTTTGCGATTAATTGAA 
                   
               
               
                   
                   
                 GTCATTGATAATGGTTTGGGCTTAGAAAAAGAAGATGTGGCT 
                   
               
               
                   
                   
                 TTGGCTTTGCGTCGTCATGCGACAAGTAAAATCAAAGATTCA 
                   
               
               
                   
                   
                 GCTGATTTATTTCGAATTAGAACGCTCGGTTTTCGGGGTGAGG 
                   
               
               
                   
                   
                 CTCTGCCGTCAATCGCTTCTGTCAGTCAGATGACGATTGAAAC 
                   
               
               
                   
                   
                 AAGTAATGCTCAGGAAGAAGCTGGGACAAAACTGATTGCTA 
                   
               
               
                   
                   
                 AAGGTGGGACGATTGAAACTTTAGAACCTCTTGCAAAGCGGT 
                   
               
               
                   
                   
                 TAGGGACAAAAATTTCTGTTGCGAATCTTTTTTATAATACACC 
                   
               
               
                   
                   
                 AGCAAGGCTCAAGTATATCAAGTCTTTACAGGCTGAACTTTC 
                   
               
               
                   
                   
                 TCATATTACAGATATTATCAATCGTTTGAGCCTCGCTCATCCA 
                   
               
               
                   
                   
                 GAGATTTCTTTTACTTTAGTTAATGAGGGTAAAGAATTTTTGA 
                   
               
               
                   
                   
                 AAACGGCGGGAAATGGAGACTTGCGCCAAGTGATTGCTGCA 
                   
               
               
                   
                   
                 ATTTATGGCATTGGAACGGCGAAAAAAATGCGTGAGATTAAT 
                   
               
               
                   
                   
                 GGCTCGGACTTAGATTTTGAACTGACAGGTTATGTCAGTTTAC 
                   
               
               
                   
                   
                 CCGAGCTGACAAGAGCGAATCGCAACTATATCACGATTTTGA 
                   
               
               
                   
                   
                 TTAATGGTCGATTTATCAAGAATTTTTTGTTGAATCGAGCAAT 
                   
               
               
                   
                   
                 TTTAGAAGGTTACGGGAACCGATTGATGGTTGGACGTTTTCCT 
                   
               
               
                   
                   
                 TTTGCTGTTTTATCAATTAAAATTGACCCTAAATTAGCAGATG 
                   
               
               
                   
                   
                 TCAATGTCCATCCGACAAAACAAGAAGTACGTTTGTCTAAGG 
                   
               
               
                   
                   
                 AACGTGAATTGATGACTTTAATTTCTAAAGCGATTGATGAGA 
                   
               
               
                   
                   
                 CCTTATCAGAAGGGGTTTTGATTCCAGAAGCTTTGGAAAATTT 
                   
               
               
                   
                   
                 GCAAGGTAGAGCCAAGGAAAAGGGGACTGTTTCTGTTCAAAC 
                   
               
               
                   
                   
                 GGAACTTCCTTTACAGAATAATCCTTTATACTATGACAATGTT 
                   
               
               
                   
                   
                 CGTCAAGATTTTTTTGTCAGAGAAGAAGCGATTTTTGAAATC 
                   
               
               
                   
                   
                 AATAAAAACGATAATTCAGATTCTCTGACTGAACAAAATTCT 
                   
               
               
                   
                   
                 ACTGATTATACAGTTAATCAGCCAGAAACTGGTTCTGTCAGT 
                   
               
               
                   
                   
                 GAAAAAATTACGGACAGAACTGTCGAAAGTTCAAATGAATTT 
                   
               
               
                   
                   
                 ACTGACAGAACCCCAAAAAATTCTGTCAGTAACTTTGGAGTT 
                   
               
               
                   
                   
                 GATTTTGATAATATTGAGAAGCTGAGTCAGCAATCAACTTTTC 
                   
               
               
                   
                   
                 CCCAACTAGAATACTTGGCACAATTGCATGCGACTTATTTACT 
                   
               
               
                   
                   
                 TTGTCAGTCAAAAGAGGGTCTTTATTTGGTTGACCAACATGC 
                   
               
               
                   
                   
                 GGCTCAGGAGCGAATCAAGTATGAATATTGGAAAGATAAAA 
                   
               
               
                   
                   
                 TCGGCGAAGTGAGCATGGAGCAACAAATTTTACTTGCGCCAT 
                   
               
               
                   
                   
                 ATTTATTTACTTTACCCAAAAATGATTTTATTGTTTTAGCTGA 
                   
               
               
                   
                   
                 GAAAAAGGATTTATTACATGAAGCAGGGGTTTTCTTGGAAGA 
                   
               
               
                   
                   
                 ATACGGAGAAAATCAATTCATATTAAGAGAGCATCCGATTTG 
                   
               
               
                   
                   
                 GTTAAAAGAAACTGAGATAGAGAAATCAATTAATGAAATGA 
                   
               
               
                   
                   
                 TTGATATTATTCTCTCATCAAAAGAATTTTCACTCAAAAAATA 
                   
               
               
                   
                   
                 TCGGCATGATTTAGCCGCAATGGTTGCTTGTAAAAGCTCAAT 
                   
               
               
                   
                   
                 CAAAGCCAACCATCCCCTTGATGCCGAGTCTGCTAGAGCTTT 
                   
               
               
                   
                   
                 GCTTAGAGAATTATCAACTTGTAAAAATCCTTATAGTTGTGCG 
                   
               
               
                   
                   
                 CATGGACGGCCAACGATTGTCCATTTTTCAGGAGATGACATT 
                   
               
               
                   
                   
                 CAAAAAATGTTCCGCAGAATTCAAGAAACGCATCGTTCAAAA 
                   
               
               
                   
                   
                 GCGGCCTCTTGGAAAGATTTTGAGTAA 
                   
               
               
                   
               
               
                   L.   lactis   
                   
                 ATGATTGAACTTAGTGGCAAAGATAGAAAGTATTTGTATAAA 
                 504 
               
               
                 dsDNA 
                   
                 CTAGTAAAATCCAAAAAACTAAATTATGAACAAGGTAATTTA 
                   
               
               
                 template 
                   
                 TCGCATCAAGTTTTAATTGAAAACAAGTTAGCAAAAGTTTAC 
                   
               
               
                 (Erythromycin 
                   
                 TTTACAAGCGATAAATATGATCCTGACTTAGGGGAACACATA 
                   
               
               
                 resistance 
                   
                 AATCCACAAAATATTATTGCTCCAACTAGTACAGGTTTAAGA 
                   
               
               
                 gene) 
                   
                 TATAAAAATATTTATCGTGAACAATTATGGGAAAAATATTTT 
                   
               
               
                 Homology 
                   
                 ACTCCTATTTGGGTATCTACGGCAACAACGACTCTAATATGGT 
                   
               
               
                 arm, promoter, 
                   
                 TAGCAAAATATTTACTAGAGAACTTGCTGTAACGCTAAGTAA 
                   
               
               
                 gene, 
                   
                 GATTACTATCCATAGCTCTTTTTTATCTTTTCTCATCTTTCCAC 
                   
               
               
                 terminator, 
                   
                 CTCCTAGCCCACTCGGGCTTTTTAATTTAAAAATTGTTTAATC 
                   
               
               
                 homology arm 
                   
                 TCATGAAACGCCATGCCTATTTCTAACAGTAAGATAATGCTG 
                   
               
               
                   
                   
                 TCAGTATAGCGCCTAAGCGTTTCTTTTTGTTCTGATTTTTTAAT 
                   
               
               
                   
                   
                 GTGGTCTTTATTCTTCAACTAAAGCACCCATTAGTTCAACAAA 
                   
               
               
                   
                   
                 CGAAAATTGGATAAAGTGGGATATTTTTAAAATATATATTTA 
                   
               
               
                   
                   
                 TGTTACAGTAATATTGACTTTTAAAAAAGGATTGATTCTAATG 
                   
               
               
                   
                   
                 AAGAAAGCAGACAAGTAAGCCTCCTAAATTCACTTTAGATAA 
                   
               
               
                   
                   
                 AAATTTAGGAGGCATATCAAATGAACAAAAATATAAAATATT 
                   
               
               
                   
                   
                 CTCAAAACTTTTTAACGAGTGAAAAAGTACTCAACCAAATAA 
                   
               
               
                   
                   
                 TAAAACAATTGAATTTAAAAGAAACCGATACCGTTTACGAAA 
                   
               
               
                   
                   
                 TTGGAACAGGTAAAGGGCATTTAACGACGAAACTGGCTAAA 
                   
               
               
                   
                   
                 ATAAGTAAACAGGTAACGTCTATTGAATTAGACAGTCATCTA 
                   
               
               
                   
                   
                 TTCAACTTATCGTCAGAAAAATTAAAACTGAATACTCGTGTC 
                   
               
               
                   
                   
                 ACTTTAATTCACCAAGATATTCTACAGTTTCAATTCCCTAACA 
                   
               
               
                   
                   
                 AACAGAGGTATAAAATTGTTGGGAGTATTCCTTACCATTTAA 
                   
               
               
                   
                   
                 GCACACAAATTATTAAAAAAGTGGTTTTTGAAAGCCATGCGT 
                   
               
               
                   
                   
                 CTGACATCTATCTGATTGTTGAAGAAGGATTCTACAAGCGTA 
                   
               
               
                   
                   
                 CCTTGGATATTCACCGAACACTAGGGTTGCTCTTGCACACTCA 
                   
               
               
                   
                   
                 AGTCTCGATTCAGCAATTGCTTAAGCTGCCAGCGGAATGCTTT 
                   
               
               
                   
                   
                 CATCCTAAACCAAAAGTAAACAGTGTCTTAATAAAACTTACC 
                   
               
               
                   
                   
                 CGCCATACCACAGATGTTCCAGATAAATATTGGAAGCTATAT 
                   
               
               
                   
                   
                 ACGTACTTTGTTTCAAAATGGGTCAATCGAGAATATCGTCAA 
                   
               
               
                   
                   
                 CTGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCC 
                   
               
               
                   
                   
                 AAAGTAAACAATTTAAGTACCGTTACTTATGAGCAAGTATTG 
                   
               
               
                   
                   
                 TCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAAT 
                   
               
               
                   
                   
                 AATATGAGATAATGCCGACTGTACTTTTTACAGTCGGTTTTCT 
                   
               
               
                   
                   
                 AATGTCACTAACCTGCCCCGTTAGTTGAAGAAGGTTTTTATAT 
                   
               
               
                   
                   
                 TACAGCTCCACGGTTAAATTTGTCGCCTGACTGTTTAAAGCTC 
                   
               
               
                   
                   
                 GTTAGACTACGATATTTTCCGCTTGTCGTAAGTTGTACAAGTA 
                   
               
               
                   
                   
                 AATCAAGAATGATTTTGTGATAGTACGGTTTAGACTGCCTGCT 
                   
               
               
                   
                   
                 TTGCATGATTGCGGTGTCTAGTTTGTTCATGGTTAGTTATCCT 
                   
               
               
                   
                   
                 TAACTTGCAAAAAAATCAAGTTAATAGTTAAAATTTTTCATC 
                   
               
               
                   
                   
                 AAGTCATAAATAGAATTTTCTTCTAAATTTGCTGCTCTTTCTA 
                   
               
               
                   
                   
                 ATTCTTTAACCTTATCAAGTGTTAATTTATTCGGAGCTAATCT 
                   
               
               
                   
                   
                 AATGCGATATAGAGCATTATATGTGATTCCCATATTCTTCGCT 
                   
               
               
                   
                   
                 ATCGCCTCATATCTTACCCCTGATTGTTTTAAAATCTCATCAA 
                   
               
               
                   
                   
                 GTGGTTTATAAGTTTTACTCATTTTATCTCCTTTCTGATTTTTA 
                   
               
               
                   
                   
                 TGTTTTTCATTCTAACATTAACTTGATTTTTATGCAAGTAATA 
                   
               
               
                   
                   
                 ACTTTACTTTTTTGCAAGTTTTCTCTTGAAAGTAGTT 
                   
               
               
                 RBS 
                   
                 TTTTGGGGAGACGACCAT 
                 505 
               
               
                   
               
               
                 RBS Mod 
                   
                 AAAAGGAGGTTTTTT 
                 506 
               
               
                   
               
               
                 RBS 2 
                   
                 AAAAAATAGGAGGAAAAACAT 
                 507 
               
               
                   
               
               
                 RBS 2 Mod 
                   
                 AAAAAAAAAAGGAGGTTTTTT 
                 508 
               
               
                   
               
               
                 RBS 1 
                   
                 AAAAATAAGGTGGAAAAACAT 
                 509 
               
               
                   
               
               
                 RBS 3 
                   
                 AAAAATAAGGAGGTAAAACAT 
                 510 
               
               
                   
               
               
                 RBS 4 
                   
                 AGCTATTCATAAGGAGGTTTAGATT 
                 511 
               
               
                   
               
               
                   L.   Lactis  MutL 
                   
                 MGKIIELNEALANQIAAGEVVERPASVVKELVENSIDAGSSKIIIN 
                 512 
               
               
                   
                   
                 VEEAGLRLIEVIDNGLGLEKEDVALALRRHATSKIKDSADLFRIR 
                   
               
               
                   
                   
                 TLGFRGEALPSIASVSQMTIETSNAQEEAGTKLIAKGGTIETLEPL 
                   
               
               
                   
                   
                 AKRLGTKISVANLFYNTPARLKYIKSLQAELSHITDIINRLSLAHP 
                   
               
               
                   
                   
                 EISFTLVNEGKEFLKTAGNGDLRQVIAAIYGIGTAKKMREINGSD 
                   
               
               
                   
                   
                 LDFELTGYVSLPELTRANRNYITILINGRFIKNFLLNRAILEGYGN 
                   
               
               
                   
                   
                 RLMVGRFPFAVLSIKIDPKLADVNVHPTKQEVRLSKERELMTLIS 
                   
               
               
                   
                   
                 KAIDETLSEGVLIPEALENLQGRAKEKGTVSVQTELPLQNNPLYY 
                   
               
               
                   
                   
                 DNVRQDFFVREEAIFEINKNDNSDSLTEQNSTDYTVNQPETGSVS 
                   
               
               
                   
                   
                 EKITDRTVESSNEFTDRTPKNSVSNFGVDFDNIEKLSQQSTFPQLE 
                   
               
               
                   
                   
                 YLAQLHATYLLCQSKEGLYLVDQHAAQERIKYEYWKDKIGEVS 
                   
               
               
                   
                   
                 MEQQILLAPYLFTLPKNDFIVLAEKKDLLHEAGVFLEEYGENQFI 
                   
               
               
                   
                   
                 LREHPIWLKETEIEKSINEMIDIILSSKEFSLKKYRHDLAAMVACK 
                   
               
               
                   
                   
                 SSIKANHPLDAESARALLRELSTCKNPYSCAHGRPTIVHFSGDDI 
                   
               
               
                   
                   
                 QKMFRRIQETHRSKAASWKDFE 
                   
               
               
                   
               
               
                   L.   Lactis  MutL 
                   
                 MGKIIELNEALANQIAAGEVVERPASVVKELVKNSIDAGSSKIIIN 
                 513 
               
               
                 E33 
                   
                 KVEEAGLRLIEVIDNGLGLEKEDVALALRRHATSKIKDSADLFRIR 
                   
               
               
                   
                   
                 TLGFRGEALPSIASVSQMTIETSNAQEEAGTKLIAKGGTIETLEPL 
                   
               
               
                   
                   
                 AKRLGTKISVANLFYNTPARLKYIKSLQAELSHITDIINRLSLAHP 
                   
               
               
                   
                   
                 EISFTLVNEGKEFLKTAGNGDLRQVIAAIYGIGTAKKMREINGSD 
                   
               
               
                   
                   
                 LDFELTGYVSLPELTRANRNYITILINGRFIKNFLLNRAILEGYGN 
                   
               
               
                   
                   
                 RLMVGRFPFAVLSIKIDPKLADVNVHPTKQEVRLSKERELMTLIS 
                   
               
               
                   
                   
                 KAIDETLSEGVLIPEALENLQGRAKEKGTVSVQTELPLQNNPLYY 
                   
               
               
                   
                   
                 DNVRQDFFVREEAIFEINKNDNSDSLTEQNSTDYTVNQPETGSVS 
                   
               
               
                   
                   
                 EKITDRTVESSNEFTDRTPKNSVSNFGVDFDNIEKLSQQSTFPQLE 
                   
               
               
                   
                   
                 YLAQLHATYLLCQSKEGLYLVDQHAAQERIKYEYWKDKIGEVS 
                   
               
               
                   
                   
                 MEQQILLAPYLFTLPKNDFIVLAEKKDLLHEAGVFLEEYGENQFI 
                   
               
               
                   
                   
                 LREHPIWLKETEIEKSINEMIDIILSSKEFSLKKYRHDLAAMVACK 
                   
               
               
                   
                   
                 SSIKANHPLDAESARALLRELSTCKNPYSCAHGRPTIVHFSGDDI 
                   
               
               
                   
                   
                 QKMFRRIQETHRSKAASWKDFE 
                   
               
               
                   
               
               
                   E.   Coli  MutL 
                   
                 MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDID 
                 514 
               
               
                   
                   
                 IERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIIS 
                   
               
               
                   
                   
                 LGFRGEALASISSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTV 
                   
               
               
                   
                   
                 KPAAHPVGTTLEVLDLFYNTPARRKFLRTEKTEFNHIDEIIRRIAL 
                   
               
               
                   
                   
                 ARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGTAFLEQA 
                   
               
               
                   
                   
                 LAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDR 
                   
               
               
                   
                   
                 LINHAIRQACEDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEV 
                   
               
               
                   
                   
                 RFHQSRLVHDFIYQGVLSVLQQQLETPLPLDDEPQPAPRSIPENR 
                   
               
               
                   
                   
                 VAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPNAQPGYQ 
                   
               
               
                   
                   
                 KQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIV 
                   
               
               
                   
                   
                 HSDCALLERDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIP 
                   
               
               
                   
                   
                 LRLKVSAEEKSALEKAQSALAELGIDFQSDAQHVTIRAVPLPLRQ 
                   
               
               
                   
                   
                 QNLQILIPELIGYLAKQSVFEPGNIAQWIARNLMSEHAQWSMAQ 
                   
               
               
                   
                   
                 AITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE 
                   
               
               
                   
               
               
                   E.   coli  MutL 
                   
                 MPIQVLPPQLANQIAAGEVVERPASVVKELVKNSLDAGATRIDI 
                 515 
               
               
                 E32K 
                   
                 DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAII 
                   
               
               
                   
                   
                 SLGFRGEALASISSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTV 
                   
               
               
                   
                   
                 KPAAHPVGTTLEVLDLFYNTPARRKFLRTEKTEFNHIDEIIRRIAL 
                   
               
               
                   
                   
                 ARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGTAFLEQA 
                   
               
               
                   
                   
                 LAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDR 
                   
               
               
                   
                   
                 LINHAIRQACEDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEV 
                   
               
               
                   
                   
                 RFHQSRLVHDFIYQGVLSVLQQQLETPLPLDDEPQPAPRSIPENR 
                   
               
               
                   
                   
                 VAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPNAQPGYQ 
                   
               
               
                   
                   
                 KQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIV 
                   
               
               
                   
                   
                 HSDCALLERDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIP 
                   
               
               
                   
                   
                 LRLKVSAEEKSALEKAQSALAELGIDFQSDAQHVTIRAVPLPLRQ 
                   
               
               
                   
                   
                 QNLQILIPELIGYLAKQSVFEPGNIAQWIARNLMSEHAQWSMAQ 
                   
               
               
                   
                   
                 AITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE 
                   
               
               
                   
               
               
                 Pa MutL 
                   
                 MSEAPRIQLLSPRLANQIAAGEVVERPASVAKELLENSLDAGSRR 
                 548 
               
               
                 (wild-type) 
                   
                 IDVEVEQGGIKLLRVRDDGRGIPADDLPLALARHATSKIRELEDL 
                   
               
               
                   
                   
                 ERVMSLGFRGEALASISSVARLTMTSRTADAGEAWQVETEGRD 
                   
               
               
                   
                   
                 MQPRVQPAAHPVGTSVEVRDLFFNTPARRKFLRAEKTEFDHLQE 
                   
               
               
                   
                   
                 VIKRLALARFDVAFHLRHNGKTIFALHEARDELARARRVGAVC 
                   
               
               
                   
                   
                 GQAFLEQALPIEVERNGLHLWGWVGLPTFSRSQPDLQYFYVNG 
                   
               
               
                   
                   
                 RMVRDKLVAHAVRQAYRDVLYNGRHPTFVLFFEVDPAVVDVN 
                   
               
               
                   
                   
                 VHPTKHEVRFRDSRMVHDFLYGTLHRALGEVRPDDQLAPPGAT 
                   
               
               
                   
                   
                 SLTEPRPTGAAAGEFGPQGEMRLAESVLESPAARVGWSGGSSAS 
                   
               
               
                   
                   
                 GGSSGYSAYTRPEAPPSLAEAGGAYKAYFAPLPAGEAPAALPES 
                   
               
               
                   
                   
                 AQDIPPLGYALAQLKGIYILAENAHGLVLVDMHAAHERITYERL 
                   
               
               
                   
                   
                 KVAMASEGLRGQPLLVPESIAVSEREADCAEEHSSWFQRLGFEL 
                   
               
               
                   
                   
                 QRLGPESLAIRQIPALLKQAEATQLVRDVIADLLEYGTSDRIQAH 
                   
               
               
                   
                   
                 LNELLGTMACHGAVRANRRLTLPEMNALLRDMEITERSGQCNH 
                   
               
               
                   
                   
                 GRPTWTQLGLDELDKLFLRGR 
                   
               
               
                   
               
               
                 Pa MutL 
                   
                 MSEAPRIQLLSPRLANQIAAGEVVERPASVAKELLKNSLDAGSR 
                 549 
               
               
                 (E36K) 
                   
                 RIDVEVEQGGIKLLRVRDDGRGIPADDLPLALARHATSKIRELED 
                   
               
               
                   
                   
                 LERVMSLGFRGEALASISSVARLTMTSRTADAGEAWQVETEGR 
                   
               
               
                   
                   
                 DMQPRVQPAAHPVGTSVEVRDLFFNTPARRKFLRAEKTEFDHL 
                   
               
               
                   
                   
                 QEVIKRLALARFDVAFHLRHNGKTIFALHEARDELARARRVGA 
                   
               
               
                   
                   
                 VCGQAFLEQALPIEVERNGLHLWGWVGLPTFSRSQPDLQYFYV 
                   
               
               
                   
                   
                 NGRMVRDKLVAHAVRQAYRDVLYNGRHPTFVLFFEVDPAVVD 
                   
               
               
                   
                   
                 VNVHPTKHEVRFRDSRMVHDFLYGTLHRALGEVRPDDQLAPPG 
                   
               
               
                   
                   
                 ATSLTEPRPTGAAAGEFGPQGEMRLAESVLESPAARVGWSGGSS 
                   
               
               
                   
                   
                 ASGGSSGYSAYTRPEAPPSLAEAGGAYKAYFAPLPAGEAPAALP 
                   
               
               
                   
                   
                 ESAQDIPPLGYALAQLKGIYILAENAHGLVLVDMHAAHERITYE 
                   
               
               
                   
                   
                 RLKVAMASEGLRGQPLLVPESIAVSEREADCAEEHSSWFQRLGF 
                   
               
               
                   
                   
                 ELQRLGPESLAIRQIPALLKQAEATQLVRDVIADLLEYGTSDRIQ 
                   
               
               
                   
                   
                 AHLNELLGTMACHGAVRANRRLTLPEMNALLRDMEITERSGQC 
                   
               
               
                   
                   
                 NHGRPTWTQLGLDELDKLFLRGR 
                   
               
               
                 EcSSB (C10) 
                   
                 PMDFDDDIPF 
                 516 
               
               
                 CFSSB (C10) 
                   
                   
                   
               
               
                 SeSSB (C10) 
                   
                   
                   
               
               
                   
               
               
                 EcSSB (C9) 
                   
                 MDFDDDIPF 
                 517 
               
               
                 CFSSB (C9) 
                   
                   
                   
               
               
                 SeSSB (C9) 
                   
                   
                   
               
               
                   
               
               
                 EcSSB (C8) 
                   
                 DFDDDIPF 
                 518 
               
               
                 CFSSB (C8) 
                   
                   
                   
               
               
                 SeSSB (C8) 
                   
                   
                   
               
               
                   
               
               
                 EcSSB (C7) 
                   
                 FDDDIPF 
                 519 
               
               
                 CFSSB (C7) 
                   
                   
                   
               
               
                 SeSSB (C7) 
                   
                   
                   
               
               
                 PaSSB (C7) 
                   
                   
                   
               
               
                   
               
               
                 PaSSB (C10) 
                   
                 YDSFDDDIPF 
                 520 
               
               
                   
               
               
                 PaSSB (C9) 
                   
                 DSFDDDIPF 
                 521 
               
               
                   
               
               
                 PaSSB (C8) 
                   
                 SFDDDIPF 
                 522 
               
               
                   
               
               
                 MsSSB (C10) 
                   
                 FSGADDEPPF 
                 524 
               
               
                   
               
               
                 MsSSB (C9) 
                   
                 SGADDEPPF 
                 525 
               
               
                   
               
               
                 MsSSB (C8) 
                   
                 GADDEPPF 
                 526 
               
               
                   
               
               
                 MsSSB (C7) 
                   
                 ADDEPPF 
                 527 
               
               
                   
               
               
                 LrSSB (C10) 
                   
                 IDLADDELPF 
                 528 
               
               
                   
               
               
                 LrSSB (C9) 
                   
                 DLADDELPF 
                 529 
               
               
                   
               
               
                 LrSSB (C8) 
                   
                 LADDELPF 
                 530 
               
               
                   
               
               
                 LrSSB (C7) 
                   
                 ADDELPF 
                 531 
               
               
                   
               
               
                 LlSSB (C10) 
                   
                 MEISDDDLPF 
                 532 
               
               
                   
               
               
                 LlSSB (C9) 
                   
                 EISDDDLPF 
                 533 
               
               
                   
               
               
                 LlSSB (C8) 
                   
                 ISDDDLPF 
                 534 
               
               
                 LrhSSB (C8) 
                   
                   
                   
               
               
                   
               
               
                 LrhSSB (C10) 
                   
                 IDISDDDLPF 
                 535 
               
               
                   
               
               
                 LrhSSB (C9) 
                   
                 DISDDDLPF 
                 536 
               
               
                   
               
               
                 LlSSB (C7) 
                   
                 SDDDLPF 
                 537 
               
               
                 LrhSSB (C7) 
                   
                   
                   
               
               
                   
               
               
                 LlSSB 
                   
                 MEISDDDIPF 
                 538 
               
               
                 C3:EcSSB 
                   
                   
                   
               
               
                   
               
               
                 LlSSB 
                   
                 MEIFDDDIPF 
                 539 
               
               
                 C7:EcSSB 
                   
                   
                   
               
               
                   
               
               
                 LlSSB 
                   
                 MEDFDDDIPF 
                 540 
               
               
                 C8:EcSSB 
                   
                   
                   
               
               
                   
               
               
                 LlSSB 
                   
                 MMDFDDDIPF 
                 541 
               
               
                 C9:EcSSB 
                   
                   
                   
               
               
                   
               
               
                 LlSSB 
                   
                 MEIFDDDIPF 
                 542 
               
               
                 C7:PaSSB 
                   
                   
                   
               
               
                   
               
               
                 LlSSB 
                   
                 MESFDDDIPF 
                 543 
               
               
                 C8:PaSSB 
                   
                   
                   
               
               
                   
               
               
                 LlSSB 
                   
                 MEIADDEPPF 
                 544 
               
               
                 C7:MsSSB 
                   
                   
                   
               
               
                   
               
               
                 LlSSB 
                   
                 MEGADDEPPF 
                 545 
               
               
                 C8:MsSSB 
                   
                   
                   
               
               
                   
               
               
                 LrSSB 
                   
                 IDLADDEPPF 
                 546 
               
               
                 C7:MsSSB 
                   
                   
                   
               
               
                   
               
               
                 LrSSB 
                   
                 EDGADDEPPF 
                 547 
               
               
                 C8:MsSSB 
               
               
                   
               
            
           
         
       
     
     Materials and Methods 
     Bacterial Strains and Culturing Conditions 
     The  E. coli  strain used was derived from EcNR2 with some modifications (EcNR2.dnaG_Q576A.tolC_mut.mutS::cat_mut.dlambda::zeoR)6.  L. lactis  strain NZ9000 was provided as a kind gift from Jan Peter Van Pijkeren.  M. smegmatis  strain mc(2)155 was purchased from ATCC. The  C. crescentus  strain used was NA1000. 
     All chemicals were purchased from Sigma Aldrich, unless stated otherwise.  E. coli  and its derivatives were cultured in Lysogeny broth—Low sodium (Lb-L) (10 g/L tryptone, 5 g/L yeast extract (Difco), PH 7.5 with NaOH), in a roller drum at 34° C.  L. lactis  was cultured in M17 broth (Difco, BD BioSciences) supplemented with 0.5% (w/v) D-glucose, static at 30° C.  M. smegmatis  was cultured in Middlebrook 7H9 Broth (Difco, BD BioSciences) with AD Enrichment (10× stock: 50 g/L BSA, 20 g/L D-glucose, 8.5 g/L NaCl), supplemented with glycerol and Tween 80 to a final concentration of 0.2% (v/v) and 0.05% (v/v), respectively, in a roller drum at 37° C.  C. crescentus  was cultured in peptone-yeast extract (PYE) broth (2 g/L peptone, 1 g/L yeast extract (Difco), 0.3 g/L MgSO4, 0.5 mM 0.5M CaCl2), shaking at 30° C. 
     Plating was done on petri dishes of LB agar for  E. coli , M17 Agar (Difco, BD BioSciences) supplemented with 0.5% (w/v) D-glucose for  L. lactis,  7H10 (Difco, BD BioSciences) supplemented with AD Enrichment and 0.2% (v/v) glycerol for  M. smegmatis , and PYE agar for  C. crescentus . Antibiotics were added to the media when appropriate, at the following concentrations: 50 μg/mL carbenicillin for  E. coli,  10 μg/mL chloramphenicol for  L. lactis , and 100m/mL hygromycin B for  M. smegmatis,  5 μg/ml kanamycin for  C. crescentus . For the selective plates used to determine allelic recombination frequency, antibiotics were added as follows: 0.005% SDS for  E. coli,  50 μg/mL rifampicin for  L. lactis,  20 μg/mL streptomycin for  M. smegmatis , and 5 μg/m1rifampicin for  C. crescentus.    
     Construction and Transformation of Plasmids 
     Plasmids were constructed using PCR fragments and Gibson Assembly. All primers and genes were obtained from Integrated DNA Technologies (IDT). Plasmids were derived from pARC8 for use in  E. coli , pjp005 for use in  L. lactis —a gift from Jan Peter Van Pijkeren, pKM444 for use in  M. smegmatis —a gift from Kenan Murphy (Addgene plasmid #108319), and pBXMCS-2 for use in  C. crescentus . Genes were codon optimized for each of the host organisms using IDT&#39;s online Codon Optimization Tool.  E. coli  and  L. lactis  plasmid constructs were Gibson assembled, then directly transformed into electrocompetent  E. coli  and  L. lactis  strains.  M. smegmatis  plasmids were first cloned in NEB 5-alpha Competent  E. coli  (New England Biolabs) for plasmid verification before transformation into electrocompetent  M. smegmatis . All cloning was verified by Sanger sequencing (Genewiz). Plasmids will be deposited in Addgene. All data is available from the authors upon reasonable request. 
     Protein Purification 
     To prepare Redβ for in vitro analysis, it was first cloned by Gibson cloning into pET-53-DEST, with a 6× poly-histidine tag followed by a glycine-serine linker and a TEV protease site (MHHHHHHGSGENLYFQG) appended to its N-terminus. After purification and treatment with TEV protease, this leaves only an N-terminal glycine before the start codon. Overnight cultures of  E. coli  BL21 (DE3) (NEB) with the expression construct were diluted 1:100 into Fernbach flasks, grown to an OD of −0.5, and induced with 1 mM IPTG at 37° C. for 4 h. Cultures were pelleted at 10,000×g in a fixed angle rotor for 10 min and the supernatant decanted. Bacterial pellets were resuspended in 30 mL of lysis buffer (150 mM NaCl, 0.1% v/v Triton-X, 50 mM TRIS-HCl pH 8.0) and sonicated at 80% power, 50% duty cycle for 5 minutes on ice. The lysed cultures were again centrifuged for 10 min at 15,000×g in a fixed angle rotor. The supernatant was then incubated for 30 minutes at room temperature with HisPur cobalt resin (Thermo) and column purified on disposable 25 ml polypropylene columns (Thermo). The protein-bound resin was washed with four column volumes of wash buffer (150 mM NaCl, 10 mM imidazole, 50 mM TRIS-HCl pH 8.0) and bound protein was eluted with two column volumes of elution buffer (150 mM NaCl, 250 mM imidazole, 50 mM TRIS-HCl pH 8.0). Protein eluates were dialyzed overnight against 25 mM TRIS-HCl pH 7.4 with 10,000 MWCO dialysis cassettes (Thermo), concentration was measured by Qubit (Thermo) and 1.5 mg of protein was cleaved in a 2 ml reaction with 240 Units of TEV protease (NEB) for two hours at 30° C. The TEV cleavage reaction was re-purified with cobalt resin, except that in this case the flow-through was collected, as the His tag and the TEV protease were bound to the resin. Expression and successful TEV cleavage were confirmed by SDS-PAGE. Protein was concentrated in 10,000 MWCO Amicon protein concentrators (Sigma), protein concentration was assayed by Qubit, and an equal volume of glycerol was added to allow storage at −20° C.  E. coli  and  L. lactis  SSBs were prepared according to previously published protocol (Lohman, Green, and Beyer, 1986) without the use of an affinity tag. 
     Oligonucleotide Annealing and Quenching Experiments 
     Fluorescent (tolC-r.null.mut-3′FAM) and quenching (tolC-f.null.mut-5′IBFQ) oligos were ordered from Integrated DNA Technologies. Unless otherwise indicated, 50 nM of each oligo was incubated in 25 mM TRIS-HCl pH 7.4 with 1.0 μM Ec_SSB or Ll_SSB at 30° C. for 30 minutes. 100 μl of each oligo mixture were then combined into a 96-well clear-bottom black assay plate (Costar), incubated a further 60 minutes at 30° C., and annealing was tracked on a Synergy H4 microplate reader (BioTek) with fluorescence excitation set to 495 nm and emission set to 520 nm. After 60 minutes, 20 μl of a solution with or without 25 μM Redβ and containing 100 mM MgCl 2  was added to achieve a final reaction concentration of 2.5 μM Redβ and 10 mM MgCl 2 . The annealing was then tracked over 10 hours in a the Synergy H4 microplate reader with the setting indicated above. 
     Preparation of Electrocompetent  E. coli    
     A single colony of  E. coli  was grown overnight to saturation. In the morning 30 μL of dense culture was inoculated into 3 mL of fresh media and grown for 1 hour. To induce gene expression of the pARC8 vector for recombineering experiments, L-arabinose was added to a final concentration of 0.2% (w/v) and the cells were grown an additional hour. 1 mL of cells were pelleted at 4° C. by centrifugation at 12,000×g for 2.5 minutes and washed twice with 1 mL of ice-cold dH 2 O. Cells were resuspended in 50 μL ice-cold dH 2 O containing DNA and transferred to a pre-chilled 0.1 cm electroporation cuvette. 
     Preparation of Electrocompetent  L. lactis    
     A single colony of  L. lactis  was grown overnight to saturation. 500 μL of dense culture was inoculated into 5 mL of fresh media, supplemented with 500 mM sucrose and 2.5% (w/v) glycine, and grown for 3 hours. To induce gene expression of the pJP005 vector for recombineering experiments, the cells were grown for an additional 30 min after adding 1 ng/mL freshly diluted nisin, unless stated otherwise. For the optimized condition ( FIG. 20B ), 10 ng/mL nisin was used. Cells were pelleted at 4° C. by centrifugation at 5,000×g for 5 minutes and washed twice with 2 mL of ice-cold electroporation buffer (500 mM sucrose containing 10% (w/v) glycerol) by centrifugation at 13,200×g for 2.5 minutes. Cells were resuspended in 80 μL ice-cold electroporation buffer containing DNA and transferred to a pre-chilled 0.1 cm electroporation cuvette. 
     Preparation of Electrocompetent  M. smegmatis    
     A single colony of  M. smegmatis  was grown overnight to saturation. The next day 25 μL of dense culture was inoculated into 5 mL of fresh media in the evening and grown overnight to an OD600 of 0.9. Cells were pelleted at 4° C. by centrifugation at 3,500×g for 10 minutes and washed twice with 10 mL ice-cold 10% glycerol. Cells were resuspended in 360 μL ice-cold 10% glycerol and transferred along with 10 μL of DNA to a pre-chilled 0.2 cm electroporation cuvette. 
     Preparation of Electrocompetent  C. crescentus    
     A single colony of  C. crescentus  was grown overnight. The next day cells were diluted back to OD ˜0.001 in 25 mL PYE, and grown overnight. The next day, 250 μL of 30% xylose was added to cells at OD ˜0.2. Cells were harvested at between OD=0.5 and OD=0.7, spun at 10,000 rpm for 10 min, and then washed twice in 12.5 ml of ice-cold dH 2 O, washed once in 12.5 ml of ice-cold 10% glycerol, then washed and resuspended in 2.5 ml of ice-cold 10% glycerol. 90 μL of cells were added along with DNA to 0.1 cm cuvettes and incubated on ice for 10 min. 
     Recombineering Experiments 
     Electrocompetent cells were electroporated with 90-mer oligos at: 1 uM for  E. coli,  50 μg for  L. lactis , and 10 uM for  C. crescentus.  70-mer oligos were used at 1 μg for  M. smegmatis . All oligos were obtained from IDT and can be found under “Oligonucleotides for genome editing” in materials and methods. For dsDNA experiments  L. lactis  was electroporated with 1.5 μg purified linear dsDNA. Cells were electroporated using a Bio-Rad gene pulser set to 25 μF, 200 S2, and 1.8 kV for  E. coli,  2.0 kV for  L. lactis , and 1.5 kV for  C. crescentus  and to 1000Ω and 2.5 kV for  M. smegmatis . Immediately after electroporation, cells were recovered in fresh media for 3 hours for  E. coli,  1 hour for  L. lactis , overnight for  M. smegmatis  and overnight for  C. crescentus. L. lactis  recovery media was supplemented with MgCl 2  and CaCl 2 ) at a concentration of 20 mM and 2 mM, respectively.  E. coli  recovery media was supplemented with carbenicillin.  M. smegmatis  recovery media was supplemented with hygromycin.  C. crescentus  recovery media was supplemented with 0.3% xylose and kanamycin. After recovery, the cells were serial diluted and plated on non-selective vs. selective agar plates to obtain approximately 50-500 CFU/plate. Colonies were counted using a custom script in Fiji, and allelic recombination frequency was calculated by dividing the number of colonies on selective plates, with the number of colonies on non-selective plates. 
     Protein Structures 
     Protein structure images ( FIG. 18A ) were downloaded from PyMOL: Schrodinger LLC, The PyMOL Molecular Graphics System, Version 1.8 (2015). 
     Example 15 
     The editing efficiency of SSAP candidates was also tested in  Agrobacterium tumefaciens  and in  Staphylococcus aureus  using the methods described above. 
     As shown in  FIG. 24 , PF071 (SEQ ID NO: 205), PF076 (SEQ ID NO: 210), PF074 (SEQ ID NO: 208), and N003 (SEQ ID NO: 3) showed an increase in editing efficiency (as indicated by enrichment on the Y-axis) relative to other SSAP candidates in  Agrobacterium tumefaciens.    
     As shown in  FIG. 25 , PF003 (SEQ ID NO: 143), SR033 (SEQ ID NO: 41), SR024 (SEQ ID NO: 32), SR041 (SEQ ID NO: 49), SR081 (SEQ ID NO: 89), and SR063 (SEQ ID NO: 71) showed an increase in editing efficiency (as indicated by enrichment on the Y-axis) relative to other SSAP candidates in  Staphylococcus aureus.