Patent Publication Number: US-11653619-B2

Title: Cotton variety FM 2202GL

Description:
CROSS-REFERENCE TO RELATED APPLICATION 
     This application claims priority to U.S. Provisional Application No. 62/994,121, filed on Mar. 24, 2020, which is hereby incorporated by reference in its entirety. 
    
    
     FIELD OF THE INVENTION 
     This invention relates to the field of plant breeding. More particularly, the invention relates to a variety of cotton designated as FM 2202GL, its essentially derived varieties and the hybrid varieties obtained by crossing FM 2202GL as a parent line with plants of other varieties or parent lines. 
     BACKGROUND OF THE INVENTION 
     Cotton is an important, fiber producing crop. Due to the importance of cotton to the textile industry, cotton breeders are increasingly seeking to obtain healthy, good yielding crops of excellent quality. 
     Cotton is commonly reproduced by self-pollination and fertilization. This type of sexual reproduction facilitates the preservation of plant and variety characteristics during breeding and seed production. The preservation of these characteristics is often important to plant breeders for producing cotton plants having desired traits. Other methods of producing cotton plants having desired traits are also used and include methods such as genetic transformation via  Agrobacterium  infection or direct transfer by microparticle bombardment. Examples of such methods are disclosed, for example, in U.S. Pub. No. 20090049564, incorporated by reference herein in its entirety. 
     Due to the environment, the complexity of the structure of genes and location of a gene in the genome, among other factors, it is difficult to predict the phenotypic expression of a particular genotype. In addition, a plant breeder may only apply his skills on the phenotype and not, or in a very limited way, on the level of the genotype. As a result, a particular plant breeder cannot breed the same variety twice using the same parents and the same methodology. Thus, a newly bred variety is an unexpected result of the breeding process. Indeed, each variety contains a unique combination of characteristics. 
     By carefully choosing the breeding parents, the breeding and selection methods, the testing layout and testing locations, the breeder may breed a particular variety type. In addition, a new variety may be tested in special comparative trials with other existing varieties in order to determine whether the new variety meets the required expectations. 
     SUMMARY OF THE INVENTION 
     The invention relates to seeds, plants, plant cells, parts of plants, cotton lint or fiber, and cotton textiles of cotton variety FM 2202GL as well as to hybrid cotton plants and seeds obtained by repeatedly crossing plants of FM 2202GL with other cotton plants, and Essentially Derived Varieties of cotton variety FM 2202GL. The invention encompasses plants and plant varieties produced by the method of derivation or essential derivation from plants of FM 2202GL and to plants of FM 2202GL reproduced by vegetative methods, including but not limited to regeneration of embryogenic cells or tissue of FM 2202GL. The invention also encompasses methods of producing cotton seeds that comprise crossing plants of cotton variety FM 2202GL either with itself or with a second, distinct cotton plant. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The invention has been obtained by a general breeding process comprising the steps outlined below. For reference, see chapter 11, “Breeding Self-Pollinated Crops by Hybridization and Pedigree Selection” in Briggs and Knowles (1967). 
     Parent plants, which have been selected for good agronomic and fiber quality traits are manually crossed in different combinations. The resulting F1 (Filial generation 1) plants are self fertilized and the resulting F2 generation plants, which show a large variability on account of optimal gene segregation, are planted in a selection field. 
     These F2 plants are observed during the growing season for health, growth vigor, plant type, plant structure, leaf type, stand ability, flowering, maturity, seed yield, boll type, boll distribution, boll size, fiber yield and fiber quality. Plants are then selected. The selected plants are harvested and the bolls analyzed for fiber characteristics and the seed cleaned and stored. This procedure is repeated in the following growing seasons, whereby the selection and testing units increase from individual plants in the F2, to multiple plant containing ‘lines’ (descending from one mother plant) in the F5 and the number of units decrease from approximately 2500 plants in the F2 to 20 lines in the F5 by selecting about 10-20% of the units in each selection cycle. 
     The increased size of the units, whereby more seed per unit is available, allows the selection and testing in replicated trials on more than one location with a different environment and a more extensive and accurate analysis of the fiber quality. 
     The lines or candidate varieties become genotypically more homozygous and phenotypically more homogeneous by selecting similar plant types within a line and by discarding the so called off-types from the very variable F2 generation on to the final F7 or F8 generation. 
     Depending on the intermediate results the plant breeder may decide to vary the procedure described above, such as by accelerating the process by testing a particular line earlier or retesting a line another year. He may also select plants for further crossing with existing parent plants or with other plants resulting from the current selection procedure. 
     By the method of recurrent backcrossing, as described by Briggs and Knowles, supra, in chapter 13, “The Backcross Method of Breeding”, the breeder may introduce a specific trait or traits into an existing valuable line or variety, while otherwise preserving the unique combination of characteristics of this line or variety. In this crossing method, the valuable parent is recurrently used to cross it at least two or three times with each resulting backcross F1, followed by selection of the recurrent parent plant type, until the phenotype of the resulting F1 is similar or almost identical to the phenotype of the recurrent parent with the addition of the expression of the desired trait or traits. 
     This method of recurrent backcrossing eventually results in an essentially derived variety, which is predominantly derived from the recurrent parent or initial variety. This method can therefore also be used to get as close as possible to the genetic composition of an existing successful variety. Thus, compared to the recurrent parent the essentially derived variety retains a distinctive trait, which can be any phenotypic trait, with the intention to profit from the qualities of that successful initial variety. 
     Depending on the number of backcrosses and the efficacy of the selection of the recurrent parent plant type and genotype, which can be supported by the use of molecular markers as described by P. Stam (2003), the genetic conformity with the initial variety of the resulting essentially derived variety may vary between 90% and 100%. The relatedness can, for example be determined by fingerprinting techniques (e.g., making use of isozyme markers and/or molecular markers such as SNP markers, AFLP markers, microsatellites, minisatellites, RAPD markers, RFLP markers and others). A plant is “closely related” to FM 2202GL if its DNA fingerprint is at least 80%, 90%, 95% or 98% identical to the fingerprint of FM 2202GL. In one embodiment, AFLP markers are used for DNA fingerprinting (Vos et al. 1995, Nucleic Acid Research 23: 4407-4414). A closely related plant may have a Jaccard&#39;s Similarity index of at least about 0.8, preferably at least about 0.9, 0.95, 0.98 or more (Pisanu et al. ISHS 2004, Acta Hort. 660). 
     Other than recurrent backcrossing, as described herein, such essentially derived variety may also be obtained by the selection from an initial variety of an induced or natural occurring mutant plant, or of an occurring variant (off-type) plant, or of a somaclonal variant plant, or by genetic transformation of regenerable plant tissue or embryogenic cell cultures of the said initial variety by methods well known to those skilled in the art, such as  Agrobacterium -mediated transformation as described by Sakhanokho et al, (2004), Reynaerts et al. (2000), Umbeck et al. (1988) and others. Examples of transgenic events transformed in this way are “LLCotton25,” USDA-APHIS petition 02-042-01p, “Cot 102,” USDA-APHIS petition 03-155-01p, and “281-24-236,” USDA-APHIS petition 03-036-01p combined with “3006-210-23,” USDA-APHIS petition 03-036-02p. 
     Information regarding these and other transgenic events referred to herein may be found at the U.S. Department of Agriculture&#39;s (USDA) Animal and Plant Health Inspection Service (APHIS) website. An “Event” is defined as a (artificial) genetic locus that, as a result of genetic engineering, carries a foreign DNA comprising at least one copy of the gene(s) of interest. Other methods of genetic transformation are well known in the art such as microprojectile bombardment. See, e.g., U.S. Publication No. 20090049564, which is incorporated by reference herein in its entirety. 
     The plants selected or transformed retain the unique combination of the characteristics of FM 2202GL, except for the characteristics (e.g., one, two, three, four or five characteristics) changed by the selection of the mutant or variant plant or by the addition of a desired trait via genetic transformation. Therefore, the product of essential derivation (i.e., an essentially derived variety), has the phenotypic characteristics of the initial variety, except for the characteristics that change as a result of the act of derivation. Plants of the essentially derived variety can be used to repeat the process of essential derivation. The result of this process is also a variety essentially derived from said initial variety. 
     In one embodiment, FM 2202GL progeny plants are produced by crossing plants of FM 2202GL with other, different or distinct cotton plants, and further selfing or crossing these progeny plants with other, distinct plants and subsequent selection of derived progeny plants. The process of crossing FM 2202GL derived progeny plants with itself or other distinct cotton plants and the subsequent selection in the resulting progenies can be repeated up to 7 or 8 times in order to produce FM 2202GL derived cotton plants. 
     FM 2202GL was derived from a back-cross breeding program with BASF experimental line 11TP0345C and the BASF variety FM 2322GL as the other parent for Glytol and LibertyLink tolerance. In 2014, the initial cross between the two parents was made in the BASF greenhouse in Memphis, Tenn. In 2014-2015, two backcrosses were made to 11TP0345C. In 2015, the BC2F2 plants were generated in the greenhouse and tested for the GL traits. In 2016, BC2F3 progeny rows were grown for seed increase in Arizona. In 2017 and 2018, 81 sister lines of this material were yield tested in several locations across the High Plains of Texas. One of the lines 15Q3TGL-0015 was selected and identified based upon performance and adaptation to the Texas High Plains. Breeder seed was produced in 2018. The selected 15Q3TGL-0015 line became BX2002GL and was tested extensively in 2019. 
     Provided herein as embodiments of the invention are seeds, plants, plant cells and parts of plants of the cotton variety FM 2202GL. Representative seeds of this variety will be deposited under rules 37 CFR 1.801 through 1.809, prior to issuance of a patent. Applicant has made a deposit of at least 2500 seeds of cotton variety FM 2202GL disclosed herein with NCIMB Ltd. Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen, AB21 9YA Scotland, on Oct. 20, 2022. The accession number for the deposit is NCIMB 44058. Access to this deposit will be made available during the pendency of the application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. The deposit will be maintained for a period of 30 years, or 5 years after the most recent request, or for the enforceable life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Applicant does not waive any rights granted under this patent or under the Plant Variety Protection Act (7 U.S.C. 2321 et seq.). 
     Plants produced by growing such seeds are provided herein as embodiments of the invention. Also provided herein are pollen or ovules of these plants, as well as a cell or tissue culture of regenerable cells from such plants. In another embodiment, the invention provides for a cotton plant regenerated from such cell or tissue culture, wherein the regenerated plant has the morphological and physiological characteristics of cotton cultivar FM 2202GL when grown in the same environmental conditions. In yet another embodiment, the invention provides methods of testing for a plant having the morphological and physiological characteristics of cotton cultivar FM 2202GL. In one embodiment, the testing for a plant having the morphological and physiological characteristics of cotton cultivar FM 2202GL is performed in the same field, under the same conditions and in the presence of plants of FM 2202GL, e.g., plants grown from the seed deposited under Accession number NCIMB 44058. In another embodiment, the characteristics to be tested are those described herein. 
     In another embodiment, the present invention provides regenerable cells for use in tissue culture of cotton cultivar FM 2202GL. The tissue culture will preferably be capable of regenerating plants having the physiological and morphological characteristics of the cotton cultivar FM 2202GL as described herein (e.g., Table 1), and of regenerating plants having substantially the same genotype as the cotton plant of the present invention. Preferably, the regenerable cells in such tissue cultures will be from embryos, protoplasts, meristematic cells, callus, pollen, leaves, anthers, pistils, roots, root tips, flowers, seeds, pods or stems. Still further, the present invention provides cotton plants regenerated from the tissue cultures of the invention. 
     Yet another aspect of the current invention is a cotton plant of the cotton variety FM 2202GL comprising at least a first transgene, wherein the cotton plant is otherwise capable of expressing all the physiological and morphological characteristics of the cotton variety FM 2202GL as described herein (e.g., Table 1). In particular-embodiments of the invention, a plant is provided that comprises a single locus conversion. A single locus conversion may comprise a transgenic gene which has been introduced by genetic transformation into the cotton variety FM 2202GL or a progenitor thereof. A transgenic or non-transgenic single locus conversion can also be introduced by backcrossing, as is well known in the art. In certain embodiments of the invention, the single locus conversion may comprise a dominant or recessive allele. The locus conversion may confer potentially any desired trait upon the plant as described herein. 
     Single locus conversions may be implemented by a plant breeding technique called backcrossing wherein essentially all of the desired morphological and physiological characteristics of a variety are recovered in addition to the characteristics conferred by the single locus transferred into the variety via the backcrossing technique. A single locus may comprise one gene, or in the case of transgenic plants, one or more transgenes integrated into the host genome at a single site (locus). 
     In a particular aspect, the invention provides for a method of introducing a single locus conversion into cotton cultivar FM 2202GL comprising: (a) crossing the FM 2202GL plants, grown from seed deposited under Accession No. NCIMB 44058, with plants of another cotton line that comprise a desired single locus to produce F1 progeny plants; (b) selecting F1 progeny plants that have the single locus to produce selected F1 progeny plants; (c) crossing the selected F1 progeny plants with the FM 2202GL plants to produce first backcross progeny plants; (d) selecting for first backcross progeny plants that have the desired single locus and the physiological and morphological characteristics of cotton cultivar FM 2202GL as described herein (e.g., Table 1), when grown in the same environmental conditions, to produce selected first backcross progeny plants; and (e) repeating steps (c) and (d) one or more times (e.g. one, two, three, four, etc. times) in succession to produce selected third or higher backcross progeny plants that comprise the desired single locus and all of the physiological and morphological characteristics of cotton cultivar FM 2202GL as described herein (e.g., Table 1), when grown in the same environmental conditions. Plants produced by this method have all of the physiological and morphological characteristics of FM 2202GL, except for the characteristics derived from the desired trait. 
     Another embodiment of the invention provides for a method of producing an essentially derived plant of cotton variety FM 2202GL comprising introducing a transgene conferring the desired trait into the plant, resulting in a plant with the desired trait and all of the physiological and morphological characteristics of cotton variety FM 2202GL when grown in the same environmental conditions. In another embodiment, the invention provides for a method of producing an essentially derived cotton plant from FM 2202GL comprising genetically transforming a desired trait in regenerable cell or tissue culture from a plant produced by the invention, resulting in an essentially derived cotton plant that retains the expression of the phenotypic characteristics of cotton variety FM 2202GL, except for the characteristics changed by the introduction of the desired trait. 
     Desired traits described herein include modified cotton fiber characteristics, herbicide resistance, insect or pest resistance, disease resistance, including bacterial or fungal disease resistance, male sterility, modified carbohydrate metabolism and modified fatty acid metabolism. Such traits and genes conferring such traits are known in the art. See, e.g., US 20090049564, incorporated by reference herein in its entirety. 
     The invention also provides for methods wherein the desired trait is herbicide tolerance and the tolerance is linked to a herbicide such as glyphosate, glufosinate, sulfonylurea, dicamba, phenoxy proprionic acid, cyclohexanedione, triazine, benzonitrile, bromoxynil or imidazalinone. 
     In one embodiment, the desired trait is insect resistance conferred by a transgene encoding a  Bacillus thuringiensis  (Bt) endotoxin, a derivative thereof, or a synthetic polypeptide modeled thereon. 
     Also included herein is a method of producing cotton seed, comprising the steps of using the plant grown from seed of cotton variety FM 2202GL, of which a representative seed sample will be deposited under Accession No. NCIMB 44058, as a recurrent parent in crosses with other cotton plants different from FM 2202GL, and harvesting the resultant cotton seed. 
     Another embodiment of this invention relates to seeds, plants, plant cells and parts of plants of cotton varieties that are essentially derived from FM 2202GL, being essentially the same as this invention by expressing the unique combination of characteristics of FM 2202GL, except for the agronomic characteristics (e.g., one, two, three, four, or five, characteristics) being different from the characteristics of FM 2202GL as a result of the act of derivation. 
     Another embodiment of this invention is the reproduction of plants of FM 2202GL by the method of tissue culture from any regenerable plant tissue obtained from plants of this invention. Plants reproduced by this method express the specific combination of characteristics of this invention and fall within its scope. During one of the steps of the reproduction process via tissue culture, somaclonal variant plants may occur. These plants can be selected as being distinct from this invention, but still fall within the scope of this invention as being essentially derived from this invention. 
     Another embodiment of the invention provides for a method of producing an inbred cotton plant derived from the cotton variety FM 2202GL comprising: (a) preparing a progeny plant derived from cotton variety FM 2202GL, a representative sample of seed of said variety having been deposited under Accession No. NCIMB 44058, by crossing cotton variety FM 2202GL with a cotton plant of a second variety; (b) crossing the progeny plant with itself or a second plant to produce a seed of a progeny plant of a subsequent generation; (c) growing a progeny plant of a subsequent generation from said seed and crossing the progeny plant of a subsequent generation with itself or a second plant; and (d) repeating steps (b) and (c) for an additional 3-10 generations with sufficient inbreeding to produce an inbred cotton plant derived from the cotton variety FM 2202GL. 
     Another embodiment of this invention is the production of a hybrid variety, comprising repeatedly crossing plants of FM 2202GL with plants of a different variety or varieties or with plants of a non-released line or lines. In practice, three different types of hybrid varieties may be produced (see e.g., Chapter 18, “Hybrid Varieties” in Briggs and Knowles, supra): 
     The “single-cross hybrid” produced by two different lines, the “three-way hybrid”, produced by three different lines such that first the single hybrid is produced by using two out of the three lines followed by crossing this single hybrid with the third line, and the “four-way hybrid” produced by four different lines such that first two single hybrids are produced using the lines two by two, followed by crossing the two single hybrids so produced. 
     Each single, three-way or four-way hybrid variety so produced and using FM 2202GL as one of the parent lines contains an essential contribution of FM 2202GL to the resulting hybrid variety and falls within the scope of this invention. 
     The invention also provides for cotton lint or fiber produced by the plants of the invention, plants reproduced from the invention, and plants essentially derived from the invention. The final textile produced from the unique fiber of FM 2202GL also falls within the scope of this invention. The invention also provides for a method of producing a commodity plant product (e.g., lint, cotton seed oil) comprising obtaining a plant of the invention or a part thereof, and producing said commodity plant product therefrom. 
     The entire disclosure of each document cited herein (e.g., US patent publications, non-patent literature, etc.) is hereby incorporated by reference. 
     
       
         
           
               
             
               
                 TABLE 1 
               
             
            
               
                   
               
               
                 Characteristics of FM 2202GL 
               
            
           
           
               
               
               
            
               
                   
                   
                 Variety 
               
            
           
           
               
               
               
               
            
               
                 Description of characteristic 
                 Possible expression/note 
                 FM 2202GL 
                 FM 2322GL 
               
               
                   
               
               
                 General Plant Type 
                   
                   
                   
               
               
                 Plant Habit 
                 spreading, intermediate, 
                 Intermediate 
                 Intermediate 
               
               
                   
                 compact 
                   
                   
               
               
                 Foliage 
                 sparse, intermediate, dense 
                 Intermediate 
                 Intermediate 
               
               
                 Stem Lodging 
                 lodging, intermediate, erect 
                 Erect 
                 Erect 
               
               
                 Fruiting Branch 
                 clustered, short, normal 
                 Normal 
                 Normal 
               
               
                 Growth 
                 determinate, intermediate, 
                 Intermediate 
                 Intermediate 
               
               
                   
                 indeterminate 
                   
                   
               
               
                 Leaf color 
                 greenish yellow, light green, 
                 Medium Green 
                 Medium Green 
               
               
                   
                 medium green, dark green 
                   
                   
               
               
                 Boll Shape 
                 Length &lt; Width, L = W, 
                 L &gt; W 
                 L &gt; W 
               
               
                   
                 L &gt; W 
                   
                   
               
               
                 Boll Breadth 
                 broadest at base, broadest at 
                 Broadest at 
                 Broadest at 
               
               
                   
                 middle 
                 Middle 
                 Middle 
               
               
                 Maturity 
                 Visual rating of percent open 
                 41.7 
                 46.7 
               
               
                   
                 bolls when all testing lines in 
                   
                   
               
               
                   
                 the test were about 40% 
                   
                   
               
               
                   
                 open 
                   
                   
               
               
                 Plant 
                   
                   
                   
               
               
                 cm. to first Fruiting Branch 
                 from cotyledonary node 
                 12.6 
                 14.1 
               
               
                 No. of nodes to 1st Fruiting 
                 excluding cotyledonary node 
                  6.2 
                  6.2 
               
               
                 Branch 
                   
                   
                   
               
               
                 Mature Plant Height in cm. 
                 cotyledonary node to 
                 77.9 
                 81.9 
               
               
                   
                 terminal 
                   
                   
               
               
                 Leaf: upper most, fully 
                   
                   
                   
               
               
                 expanded leaf 
                   
                   
                   
               
               
                 Type 
                 normal, sub-okra, okra, 
                 Normal 
                 Normal 
               
               
                   
                 super-okra 
                   
                   
               
               
                 Pubescense 
                 absent, sparse, medium, 
                 Medium 
                 Sparse 
               
               
                   
                 dense 
                   
                   
               
               
                 Nectaries 
                 present, absent 
                 Present 
                 Present 
               
               
                 Stem Pubescense 
                 glabrous, intermediate, hairy 
                 Intermediate 
                 Intermediate 
               
               
                 Glands (Gossypol) 
                 absent, sparse, normal, more 
                   
                   
               
               
                   
                 than normal 
                   
                   
               
               
                 Leaf 
                   
                 Normal 
                 Normal 
               
               
                 Stem 
                   
                 Normal 
                 Normal 
               
               
                 Calyx lobe 
                 (normal is absent) 
                 Normal 
                 Normal 
               
               
                 Flower 
                   
                   
                   
               
               
                 Petals 
                 cream, yellow 
                 Cream 
                 Cream 
               
               
                 Pollen 
                 cream, yellow 
                 Cream 
                 Cream 
               
               
                 Petal Spot 
                 present, absent 
                 Absent 
                 Absent 
               
               
                 Seed 
                   
                   
                   
               
               
                 Seed Index 
                 g/100 seed fuzzy basis 
                  8.6 
                  9.7 
               
               
                 Lint Index 
                 g lint/100 seeds 
                   
                   
               
               
                 Boll 
                   
                   
                   
               
               
                 Lint percent, picked 
                   
                  0.46 
                  0.45 
               
               
                 Gin Turnout, picked 
                   
                   
                   
               
               
                 Number of Seeds per Boll 
                   
                 32.2 
                 28.1 
               
               
                 Grams Seed Cotton per Boll 
                   
                  5.7 
                  5.5 
               
               
                 Number of Locules per Boll 
                   
                  4.6 
                  4.5 
               
               
                 Boll Type 
                 storm proof, storm resistant, 
                 Storm Resistant 
                 Storm Resistant 
               
               
                   
                 open 
                   
                   
               
               
                 Fiber Properties 
                   
                   
                   
               
               
                 HVI method 
                   
                   
                   
               
               
                 Length, inches, 2.5% SL 
                   
                  1.13 
                  1.15 
               
               
                 Uniformity (%) 
                   
                 82.7 
                 82.0 
               
               
                 Strength, T1 (g/tex) 
                   
                 30.9 
                 30.8 
               
               
                 Elongation, E1 (%) 
                   
                  7.2 
                  6.3 
               
               
                 Micronaire 
                   
                  4.40 
                  4.30 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2 
               
               
                   
               
               
                 Diseases 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Bacterial Blight (Race 18) 
                 Moderately Resistant 
               
               
                   
                 Verticillium Wilt 
                 Moderately Resistant 
               
               
                   
                   
               
            
           
         
       
     
     DEPOSIT INFORMATION 
     Applicant has made a deposit of at least 2500 seeds of cotton variety FM 2202GL disclosed herein with under Accession No. NCIMB 44058. Seed of cotton variety FM 2202GL is located at the BASF Maricopa Cotton Breeding Station, 880 N Power Road, Bapchule, Ariz. 85121. The lot number for this seed material is ZA9AJ6034F. Access to this deposit will be available during the pendency of the application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. The deposit will be maintained for a period of 30 years, or 5 years after the most recent request, or for the enforceable life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Upon allowance of any claims in the application, the Applicants will make available to the public, pursuant to 37 C.F.R. 1.808, sample(s) of the deposit. Applicant does not waive any rights granted under this patent or under the Plant Variety Protection Act (7 U.S.C. 2321 et seq.). 
     DEFINITIONS 
     In the description and tables which follow, a number of terms are used. In order to provide a clear and consistent understanding of the specification and claims, the following definitions are provided: 
     A: When used in conjunction with the word “comprising” or other open language in the claims, the words “a” and “an” denote “one or more.” 
     Allele: Any of one or more alternative forms of a gene locus, all of which alleles relate to one trait or characteristic. In a diploid cell or organism, the two alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes. 
     Backcrossing: A process in which a breeder repeatedly crosses hybrid progeny, for example a first-generation hybrid (F1), back to one of the parents of the hybrid progeny. 
     Backcrossing can be used to introduce one or more single locus conversions from one genetic background into another. 
     Cm to FFB: Measure of centimeters to first fruiting branch. 
     COT102: the man-made cotton locus containing the vip3A gene and further described in APHIS petition No. 03-155-01p. 
     Crossing: The mating of two parent plants. 
     Cross-pollination: Fertilization by the union of two gametes from different plants. 
     Desired Agronomic Characteristics: Agronomic characteristics (which will vary from crop to crop and plant to plant) such as yield, maturity, pest resistance and lint percent which are desired in a commercially acceptable crop or plant. For example, improved agronomic characteristics for cotton include yield, maturity, fiber content and fiber qualities. 
     Diploid: A cell or organism having two sets of chromosomes. 
     Disease Resistance: The ability of plants to restrict the activities of a specified pest, such as an insect, fungus, virus, or bacterial. 
     Disease Tolerance: The ability of plants to endure a specified pest (such as an insect, fungus, virus or bacteria) or an adverse environmental condition and still perform and produce in spite of this disorder. 
     Donor Parent: The parent of a variety which contains the gene or trait of interest which is desired to be introduced into a second variety. 
     E1: Refers to elongation, a measure of fiber elasticity (high=more elastic). 
     Emasculate: The removal of plant male sex organs or the inactivation of the organs with a cytoplasmic or nuclear genetic factor conferring male sterility or a chemical agent. 
     Essentially all the physiological and morphological characteristics: A plant having essentially all the physiological and morphological characteristics means a plant having the physiological and morphological characteristics, except for the characteristics derived from the desired trait. 
     F 1  Hybrid: The first-generation progeny of the cross of two nonisogenic plants. 
     Fallout (F 0 ): As used herein, the term “fallout” refers to the rating of how much cotton has fallen on the ground at harvest. 
     FB5 cm to FFN: Measure of centimeters from main stem to first fruiting node at fruiting branch 5. 
     2.5% Fiber Span Length: Refers to the longest 2.5% of a bundle of fibers expressed in inches as measured by a digital fibergraph. 
     Fiber Characteristics: Refers to fiber qualities such as strength, fiber length, micronaire, fiber elongation, uniformity of fiber and amount of fiber. 
     Fiber Elongation: Sometimes referred to as E1, refers to the elongation of the fiber at the point of breakage in the strength determination as measured by High Volume Instrumentation (HVI). 
     Fiber Span Length: The distance spanned by a specific percentage of fibers in a test specimen, where the initial starting point of the scanning in the test is considered 100 percent as measured by a digital fibergraph. 
     Fiber Strength (Str): Denotes the force required to break a bundle of fibers. Fiber strength is expressed in grams per tex on an HVI. 
     Fruiting Nodes: The number of nodes on the main stem from which arise branches that bear fruit or boll in the first position. 
     Genotype: The genetic constitution of a cell or organism. 
     Gin Turnout: Refers to fraction of lint in a machine harvested sample of seed cotton (lint, seed, and trash). 
     GLT: a breeding stack of GlyTol together with Twinlink. 
     Glytol: the man-made cotton locus containing the 2mepsps gene and further described in APHIS petition No. 05-217-05n (referred to as GHB614). 
     GLTP: a breeding stack of GlyTol together with Twinlink and COT102. 
     Haploid: A cell or organism having one set of the two sets of chromosomes in a diploid. 
     Length (Len): The fiber length in inches using an HVI. 
     Linkage: A phenomenon wherein alleles on the same chromosome tend to segregate together more often than expected by chance if their transmission was independent. 
     Lint Index: The weight of lint per seed in milligrams. 
     Lint Percent: The percentage of the seed cotton that is lint, handpicked samples. 
     Lint Yield: Refers to the measure of the quantity of fiber produced on a given unit of land. Presented below in pounds of lint per acre. 
     Lint/boll: As used herein, the term “lint/boll” is the weight of lint per boll. 
     Maturity Rating: A visual rating near harvest on the amount of open boils on the plant. The rating range is from 1 to 5, 1 being early and 5 being late. 
     Micronaire (Mic): Refers to a measure of fiber fineness (high=coarse fiber) as measured with an HVI machine. Within a cotton cultivar, micronaire is also a measure of maturity. 
     Micronaire differences are governed by changes in perimeter or in cell wall thickness, or by changes in both. Within a variety, cotton perimeter is fairly consistent and maturity will cause a change in micronaire. Consequently, micronaire has a high correlation with maturity within a variety of cotton. Maturity is the degree of development of cell wall thickness. 
     Mr: Fiber maturity ratio. 
     Phenotype: The detectable characteristics of a cell or organism, which characteristics are the manifestation of gene expression. 
     Plant Height: The average height in meters of a group of plants. 
     Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer to genetic loci that control to some degree numerically representable traits that are usually continuously distributed. 
     Recurrent Parent: The repeating parent (variety) in a backcross breeding program. The recurrent parent is the variety into which a gene or trait is desired to be introduced. 
     Regeneration: The development of a plant from tissue culture. 
     Seed/boll: Refers to the number of seeds per boll, handpicked samples. 
     Seedcotton/boll: Refers to the weight of seedcotton per boll, handpicked samples. 
     Seedweight: Refers to the weight of 100 seeds in grams. 
     Self-pollination: The transfer of pollen from the anther to the stigma of the same plant or a plant of the same genotype. 
     Single Locus Converted (Conversion) Plant: Plants which are developed by a plant breeding technique called backcrossing wherein essentially all of the desired morphological and physiological characteristics of a variety are recovered in addition to the characteristics conferred by the single locus transferred into the variety via the backcrossing technique. A single locus may comprise one gene, or in the case of transgenic plants, one or more transgenes integrated into the host genome at a single site (locus). 
     Stringout Rating: also referred to as “Storm Resistance”, refers to a visual rating prior to harvest of the relative looseness of the seed cotton held in the boll structure on the plant. The rating values are from 1 to 5 (tight to loose in the boll). 
     Substantially Equivalent: A characteristic that, when compared, does not show a statistically significant difference (e.g., p=0.05) from the mean. 
     T1: A measure of fiber strength, grams per tex (high=stronger fiber). 
     Tissue Culture: A composition comprising isolated cells of the same or a different type or a collection of such cells organized into parts of a plant. 
     Transgene: A genetic locus comprising a sequence which has been introduced into the genome of a cotton plant by transformation. 
     Twinlink: a breeding stack of the man-made cotton locus containing the cry2Ae gene and further described in APHIS petition No. 08-340-01p (referred to as GHB119) in combination with the man-made cotton locus containing the cry1Ab gene and further described in APHIS petition No. 08-340-01p (referred to as T304-40). 
     Twinlink Plus: a breeding stack of GlyTol together with Twinlink and COT102. 
     Uniformity Ratio (Ur): The proportion of uniform length fibers. The uniformity ratio is determined by dividing the 50% fiber span length by the 2.5% fiber span length. 
     Vegetative Nodes: The number of nodes from the cotyledonary node to the first fruiting branch on the main stem of the plant. 
     CITED REFERENCES 
     
         
         Lawrence P. Burdett, “Cotton Variety 02T15,” U.S. Pub. No. 20090049564. 
         F. N. Briggs, and P. F Knowles, 1967: “Introduction to Plant Breeding”, Rheinhold Publishing Corporation. 
         H. F. Sakhanoko et al 2004: “Induction of Somatic embryogenesis and Plant Regeneration in Select Georgia and Pee Dee Cotton Lines”, Crop Science 44: 2199-2205. 
         Umbeck et al 1988: “Genetic engineering of cotton plants and lines”, Patent application number EP0290355. 
         Reynaerts et al 2000: “Improved method for  Agrobacterium  mediated transformation of cotton”, Patent application number WO 0071733. 
         P. Stam, 2003: “Marker-assisted introgression: speed at any cost?” Proceedings of the Eucarpia Meeting on Leafy Vegetable Genetics and Breeding, Noordwijkerhout, The Netherlands, 19-21 Mar. 2003. Eds. Th. J. L. van Hintum, A. Lebeda, D. Pink, J. W. Schut. P117-124. 
         Trolinder et al. “Herbicide tolerant cotton plants having event EE-GH1.” U.S. Pat. No. 6,818,807 (2004).