Patent Publication Number: US-2020292569-A1

Title: Dispenser stages

Description:
BACKGROUND 
     Media dispensers or fluid dispensers may be utilized to dispense precise quantities of media or fluid. The fluid dispensers may be utilized for dispensing a specific quantity of liquid at a specific location of a substrate. For example, a substrate may include a plurality of wells that may each be utilized separately to perform a separate experiment. In this example, a specific quantity of a particular liquid may be deposited into each of the plurality of wells. In some examples, when a specific well of the plurality of wells is not provided with the specific quantity of the particular liquid an experiment may be compromised. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  illustrates an example dispenser stage consistent with the present disclosure. 
         FIG. 2  illustrates an example dispenser stage and an example system for a dispenser stage consistent with the present disclosure. 
         FIG. 3  illustrates an example dispenser stage and an example system for a dispenser stage consistent with the present disclosure. 
     
    
    
     DETAILED DESCRIPTION 
     As described herein, media dispensers or dispenser systems can be utilized to dispense a specific quantity of media or fluid on to a substrate. In some examples, the substrate can be coupled to a substrate nest. The substrate nest can be aligned with and coupled to a stage. In some examples, the stage can be utilized to align the substrate nest and/or substrate coupled to the substrate nest with a dispense head. In some examples, aligning the substrate nest and/or the substrate with the dispense nozzle can allow the media dispensers or dispenser systems to dispense the media or liquid at a specific location or locations on the substrate or substrate nest. 
     In some examples, the dispenser stages described herein can be utilized to align a plurality of different substrate nests and/or substrates with the dispense nozzle. In this way, a first substrate nest and/or substrate can be removed from the dispenser stage and a second substrate nest and/or substrate can be attached to the dispenser stage and aligned with the dispense nozzle by the dispenser stage. The dispenser stages described herein can provide the flexibility of using a plurality of different substrate nests and/or substrates with the media dispensers or dispenser systems. In addition, each of the plurality of different substrate nests and/or substrates can be aligned with the dispense nozzle by the dispenser stage without adjusting the dispenser stage, substrate nest, and/or substrate. 
     A number of systems and devices for dispenser stages are described herein. In some examples, dispenser stages described herein may include a dispenser stage comprising: a stage coupled to a rail system to move the stage in a first plane and a second plane, an alignment feature coupled to the stage to align a substrate nest coupled to the stage with a dispenser positioned above the stage, and a releasable fastener to couple the substrate nest to the stage. In some examples, the dispenser stages described herein may be utilized to accept and align a plurality of different substrate nests. In these examples, a plurality of different substrate nests may be utilized with a single dispenser stage. In this way, a single dispenser and dispenser stage may support a plurality of different substrate nests for different uses of the dispenser. As used herein, a substrate nest can include a device for coupling and/or aligning a substrate. As used herein, a substrate can include a material on which a liquid or media is deposited by a dispenser device. 
     As used herein, a dispenser may include a fluidic dispenser that may dispense a fluid onto a substrate. In some examples, the dispenser may be a digital fluidic dispenser that may dispense a specific quantity of fluid at a specific location of the substrate. For example, the dispenser described herein may include a device that is programmable for dispensing a specific quantity of a liquid on a specific location of a particular substrate positioned on a substrate nest. In this example, the substrate may be changed to a different type of substrate and coupled to the same substrate nest. In this example, the dispenser may be utilized to dispense a specific quantity of liquid at specific locations on the different type of substrate coupled to the substrate nest. 
     In this way, dispenser stages described herein may be utilized to receive and align a plurality of different substrate nests with a single stage to allow a single dispenser to be utilized with a plurality of different substrates and/or substrate nests without having to realign each of the substrate nests or substrates with the dispense head or nozzle of the dispenser. 
     The figures herein follow a numbering convention in which the first digit corresponds to the drawing figure number and the remaining digits identify an element or component in the drawing. Elements shown in the various figures herein may be capable of being added, exchanged, and/or eliminated so as to provide a number of additional examples of the present disclosure. In addition, the proportion and the relative scale of the elements provided in the figures are intended to illustrate the examples of the present disclosure, and should not be taken in a limiting sense. 
       FIG. 1  illustrates an example dispenser stage  100  consistent with the present disclosure. In some examples, the dispenser stage  100  may be coupled to a dispenser device. For example, the dispenser device may include a dispense head for dispensing a media or liquid on to the dispenser stage  100 . 
     In some examples, the dispenser stage  100  may be coupled to a rail system of the dispenser device to move the dispenser stage  100  in a first plane (e.g., x-direction, a first direction, etc.) and a second plane (e.g., y-direction, a second direction, etc.). In some examples, a dispense head or nozzle of the dispenser device may be in a fixed position in relation to the first plane and the second plane. That is, the dispense head of the dispenser device may not be able to move in the first plane or the second plane. In these examples, the rail system of the dispenser device may move to a specific location such that the dispense head of the dispenser device may deposit a quantity of fluid on the specific location without having to move along the first plane or the second plane. 
     In some examples, the dispenser stage  100  may include a stage  102  coupled to a base. In some examples, the stage  102  may be a platform for supporting other elements of the dispenser stage  100 . In some examples, the stage  102  may be coupled to a base portion that may cover or enclose a rail system of the dispenser device. In some examples the base or the stage  102  may be coupled to the rail system of the dispenser device such that the rail system may move the stage  102  in the first plane and/or second plane as described herein. 
     In some examples, the dispenser stage  100  may include a fastener plate  104 . In some examples, the fastener plate  104  may be coupled to the surface of the stage  102 . In some examples, the fastener plate  104  may be permanently or semi-permanently coupled to the surface of the stage  102 . For example, the fastener plate  104  may be permanently coupled to the surface of the stage  102  utilizing a welding technique. In another example, the fastener plate  104  may be semi-permanently coupled to the surface of the stage  102  utilizing an attachment device such as a screw or bolt that may pass through apertures of the fastener plate  104  and be received by apertures or threaded apertures of the stage  102 . 
     In some examples, the dispenser stage  100  may include a releasable fastener such as a magnetic fastener  108 . In some examples, the magnetic fastener  108  may be coupled to the fastener plate  104 . In some examples, the fastener plate  104  may include a slot to receive the magnetic fastener  108 . In some examples, the slot may be an aperture that exposes a portion of the stage  102  to couple the magnetic fastener  108  to the stage  102  while the slot surrounds the magnetic fastener  108 . For example, the slot may be the same or similar shape as the magnetic fastener  108  to allow the magnetic fastener  108  to be more flush with a surface (e.g. level with the surface, even with the surface, etc.) of the fastener plate  104 . In addition, the slot surrounding the magnetic fastener  108  may ensure that the magnetic fastener does not move relative to the fastener plate  104  in the first plane or the second plane as described herein. In some examples, the magnetic fastener  108  can be replaced with a different type of releasable fastener. For example, the fastener can include an aperture to receive a screw to couple a substrate nest  112  from the plurality of different substrate nest types to the stage  102 . 
     In some examples, the magnetic fastener  108  may be permanently or semi-permanently coupled to the surface of the stage  102 . For example, the magnetic fastener  108  may be permanently coupled to the surface of the stage  102  by a welding technique. In another example, the magnetic fastener  108  may be semi-permanently coupled to the surface of the stage  102  with a number of screws or bolts that may pass through a number of apertures of the magnetic fastener  108  and be received by a number of apertures or threaded apertures of the stage  102 . In this example, the number of screws or bolts may couple the magnetic fastener  108  to the stage  102  and prevent the magnetic fastener  108  from moving in a z-plane (e.g., upward direction as illustrated in  FIG. 1 , direction away from the stage  102 , etc.). In this example, the slot of the fastener plate  104  may prevent the magnetic fastener  108  from moving in an x-plane along the surface of the stage  102  or a y-plane along the surface of the stage  102 . 
     In some examples, the dispenser stage  100  may include a ferromagnetic fastener  110 . In some examples, the ferromagnetic fastener  110  may include a plate that is coupled to a substrate nest  112  such that a position of the ferromagnetic fastener  110  corresponds to a position of the magnetic fastener  108 . As used herein, the ferromagnetic fastener  110  includes a plate that comprises a ferromagnetic material. As used herein, a ferromagnetic material is a material that is attracted to a magnet. As used herein, a magnet is a material that produces a magnetic field that may attract or alter a position of a ferromagnetic material (e.g., iron, nickel, cobalt, alloys of rare-earth metals, etc.). In some examples, the ferromagnetic fastener  110  can be replaced with a plate that can include a number of apertures to receive a screw or bolt to couple the fastener to the substrate nest  112 . 
     In some examples, the ferromagnetic fastener  110  may be permanently or semi-permanently coupled to a particular side of a substrate nest  112 . For example, the ferromagnetic fastener  110  may be coupled to a bottom side as illustrated in  FIG. 1  or side of the substrate nest  112  that does not include a substrate. In some examples, each of a plurality of different substrate nests may include a ferromagnetic fastener  110  that is positioned to align a substrate nest (e.g., substrate nest  112 , etc.) with the magnetic fastener  108 . 
     In some examples, the dispenser stage  100  may include a number of alignment features  106 - 1 ,  106 - 2 , collectively referred to as alignment features  106 . In some examples, the alignment features  106  may include pins or cylindrical structures. For example, the alignment features  106  may be cylindrical pins that may be positioned within corresponding apertures of the fastener plate  104 . In this example, the pins may be permanently or semi-permanently coupled within the apertures of the fastener plate  104  such that a portion of the pins are exposed and protrude above the surface of the fastener plate  104 . In this example, the exposed portion of the pins may be received by corresponding apertures of the substrate nest  112 . That is, the alignment features  106  may align the substrate nest  112  at a specific location and orientation with the stage  102 . That is, the alignment features  106  may be positioned between the stage  102  and the substrate nest  112 . 
     In some examples, the alignment features  106  may reposition the substrate nest  112  when the ferromagnetic fastener  110  interacts and is coupled to the magnetic fastener  108 . For example, the alignment features  106  may prevent the substrate nest  112  from being out of alignment. In this example, the alignment features  106  may prevent a coupling between the substrate nest  112  and the stage  102  when the alignment features  106  are not aligned with corresponding apertures of the substrate nest  112 . 
     In this way, a substrate nest  112  may be coupled and aligned simultaneously utilizing the magnetic fastener  108  and the alignment features  106 . In a similar way, the substrate nest  112  may be removed by applying a force that is stronger than a force between the magnetic fastener  108  and the ferromagnetic fastener  110  and a different substrate nest may be coupled in a similar way. Thus, the dispenser stage  100  may be utilized to couple and align a plurality of different substrate nests (e.g., substrate nest  112 , etc.) to be utilized with a dispenser device as described herein. 
       FIG. 2  illustrates an example dispenser stage  200  and an example system  220  for a dispenser stage  200  consistent with the present disclosure. In some examples, the dispenser stage  200  may be utilized with the system  220 . In some examples, the system  220  may be utilized as a dispenser device. For example, the system  220  may be utilized to deposit a fluid or media on to a surface of a substrate coupled to the substrate nest  212  as described herein. 
     In some examples, the substrate nest  212  is coupled at a specific location and orientation of the stage  202  such that the fluid or media deposited on the surface of the substrate of the substrate nest  212  are deposited at a correct location on the substrate. For example, the substrate nest  212  may be a nest for a microtiter well plate substrate that includes a plurality wells that can each receive a fluid from a dispense head  228  of the system  220 . As used herein, a dispense head  228  can include a single nozzle to dispense a liquid or media and/or the dispense head  228  can include a plurality of nozzles that can dispense the liquid or media. In this example, the substrate nest  212  may be aligned in a specific location and orientation of the stage  202  as described herein such that the system  220  may deposit a fluid or media at a specific well of the microtiter well plate substrate coupled to the substrate nest  212 . 
     In some examples, the substrate nest  212  may be coupled to the stage  202  as described herein. For example, the substrate nest  212  may be coupled to a ferromagnetic fastener  210  that may interact with a magnetic fastener  208  when the magnetic fastener  208  is coupled to the stage  202  within a slot of a fastener plate  204  that is coupled to the stage  202 . As described herein, the fastener plate  204  may include a number of apertures to receive corresponding alignment features  206 - 1   206 - 2 , referenced herein as alignment features  206 . 
     In some examples, the alignment features  206  may align a plurality of different substrate nest types with the dispenser dispense head  228  positioned above the stage  202 . As used herein, the dispense head  228  is positioned above the stage  202  when a height of the dispense head  228  is greater than a height of the stage  202 . In some examples, a height of a nozzle or dispense head  228  may be adjustable based on a substrate nest type. In some examples, the alignment features  206  may include dowel pins. For example, the stage  202  may include an aperture of the fastener plate  204  to receive a plurality of dowel pins. As used herein, a dowel pin may include a pin, usually round or cylindrical, fitting into holes in two adjacent pieces to prevent their slipping or to align the two adjacent pieces. In some examples, the plurality of dowel pins may act as the alignment feature  206  to align a substrate nest  212  from the plurality of different substrate nest types with the dispense head  228  as illustrated in system  220 . 
     As described herein, the alignment features may be positioned within the apertures of the fastener plate  204  and protrude from the surface of the fastener plate  204  to receive corresponding apertures of the substrate nest  212  to align the substrate nest  212  with the stage  202 . In this way, the substrate of the substrate nest  212  may be aligned with a specific location of the stage  202  to allow the system  220  to precisely deposit a fluid or media at specific locations on the substrate of the substrate nest  212 . In addition, the alignment features  206  may align a plurality of different substrate nests (e.g., substrate nest  212 , etc.) to be utilized with the same stage  202  of the system  220 . 
     In some examples, the system  220  may be a dispenser device as described herein. For example, the system  220  may be utilized to deposit fluid or media on a substrate that is coupled to a substrate nest  212  as described herein. In some examples, the system  220  may include a platform  222  that may act as a base for the system  220 . The platform  222  may include a slot below an enclosure of the stage  202 . 
     In some examples, the slot of the platform  222  may connect the dispenser stage  200  to a rail system for moving the dispenser stage  200  in across the platform  222 . For example, the rail system may move the dispenser stage  200  in an x-plane or x-direction as illustrated by the compass  223  and/or the rail system may move the dispenser stage  200  in a y-plane or y-direction as illustrated by the compass  223 . In this way, the rail system may move the dispenser stage  200  to specific locations on the platform  222  to receive deposited fluid or media from a dispense head  228  of the system positioned above the dispenser stage  200 . 
     In some examples, the system  220  may include a pillar  224  coupled to the platform  222 . In some examples, the pillar  224  may raise a position of a bridge  226  coupled to the pillar  224 . In some examples, the pillar  224  may be coupled to the bridge  226  to extend a dispense head  228  of the system  220  over a position of the platform  222 . In some examples, the pillar  224  may be utilized to adjust a height of the bridge  226  and/or adjust the height of the dispense head  228  compared to a level of the platform  222  and/or substrate nest  212 . In some examples, the pillar  224  may be utilized to move the bridge  226  and/or the dispense head  228  in a z-plane or z-direction as illustrated by the compass  223 . 
     In some examples, the system  220  may be utilized to deposit a specific quantity of liquid or media at a specific location on a substrate coupled to the substrate nest  212  of the dispenser stage  200 . For example, the substrate nest  212  may be a well plate substrate nest that can couple a well plate substrate (e.g., microtiter well plate, etc.) that includes a plurality of wells that may hold a particular quantity of liquid. In this example, the plurality of wells of the well plate substrate may be relatively close to other wells. In this example, the height of the dispense head  228  may be adjusted by the pillar  224  to avoid spillage of liquid or media from a first well of the plurality of wells to a second well of the plurality of wells. 
     In some examples, the dispenser stage  200  can include a stage  202  coupled to a rail system to move the stage  202  along a first plane and a second plane of a platform  222 , a fastener coupled to the stage to couple a plurality of different substrate nest types to the stage  202 , and an alignment feature  206  to align each of the plurality of different substrate nest types with the stage  202  and a microfluidic dispenser (e.g., system  220 , etc.), wherein the microfluidic dispenser is adjustable along a third plane based on a substrate nest type coupled to the stage  202 . 
     As described herein, the dispenser stage  200  may be utilized to receive and align a plurality of different substrate nests and/or receive and align a plurality of different types of substrate nests that can be utilized to couple different types of substrates. For example, the dispenser stage  200  may be utilized to receive and align a well plate substrate nest, a dish substrate nest, a flat bottom container substrate nest, a microscope slide substrate nest, a paper substrate nest, a Petri dish substrate nest, and/or other types of substrate nests that may be utilized to receive the fluid or media from the dispense head  228  of the system  220 . As described herein, the system  220  may determine the type of substrate nest coupled to the dispenser stage  200  and alter a height of the bridge  226  or dispense head  228  based on the type of substrate nest. In addition, the alignment features  206  may be utilized to align the particular type of substrate nest coupled to the dispenser stage  200  to be positioned to receive the fluid or media dispensed by the dispense head  228 . 
     In some examples, the system  220  may be a microfluidic dispenser that may include a computing device to deposit a specific quantity of fluid or media at a specific location on a substrate of the substrate nest  212 . In addition, a substrate nest  212  may be non-mechanically removed (e.g., removed without tools, removed without removing mechanical devices such as screws or bolts, etc.) from the stage  202  and replaced with a different substrate nest (e.g., substrate nest different than substrate nest  212 , etc.) that is aligned by the alignment features  206  as described herein. Thus, the system  220  may change the type of substrate nest  212  coupled to the stage  202  and align the substrate nest  212  coupled to the stage  202  more quickly and easily compared to previous systems and methods that utilize mechanical devices to couple and align a substrate nest  212  to a stage  202 . 
       FIG. 3  illustrates an example dispenser stage  300  and an example system  320 - 1 ,  320 - 2 ,  320 - 3  for a dispenser stage consistent with the present disclosure. In some examples, the dispenser stage  300  may be the same or similar as dispenser stage  100  as referenced in  FIG. 1  and/or dispenser stage  200  as referenced in  FIG. 2 . In some examples, the dispenser stage  300  may be utilized with the system  320 - 1 ,  320 - 2 ,  320 - 3 , collectively referenced as system  320 . In some examples, the system  320  may be utilized as a dispenser device. For example, the system  320  may be a microfluidic dispenser that may be utilized to deposit a fluid or media on to a surface of substrate  314  coupled to a substrate nest  312  as described herein. 
     As described herein, the substrate nest  312  may be coupled at a specific location and orientation of the stage  302  such that the fluid or media deposited on the surface of the substrate  314  coupled to the substrate nest  312  are deposited at a correct location on the substrate  314 . For example, the substrate nest  312  may be a well substrate nest that can couple a well plate substrate  314  that includes a plurality wells that may each receive a fluid from a dispense head or nozzle of the system  320 . In this example, the substrate nest  312  may be aligned in a specific location and orientation of the stage  302  as described herein such that the system  320  may deposit a fluid or media at a specific well of the well plate substrate  314  coupled to the substrate nest  312 . A used herein, an orientation of the substrate nest  312  may include an angle of the substrate nest  312  compared to a position of the stage  302  and/or platform of the system  320 . 
     In some examples, the substrate nest  312  may be coupled to the stage  302  as described herein. For example, the substrate nest  312  may be coupled to a ferromagnetic fastener  310  that may interact with a magnetic fastener  308  when the magnetic fastener  308  is coupled to the stage  302  within a slot  334  of a fastener plate  304  that is coupled to the stage  302 . As described herein, the fastener plate  304  may include a number of apertures  332 - 1 ,  332 - 2 , referenced herein as apertures  332  to receive corresponding alignment features  306 - 1   306 - 2 , referenced herein as alignment features  306 . 
     As described herein, the alignment features  306  may be positioned within the apertures  332  of the fastener plate  304  and protrude from the surface of the fastener plate  304  to receive corresponding apertures of the substrate nest  312  to align the substrate nest  312  with the stage  302 . In this way, the substrate  314  of the substrate nest  312  may be aligned with a specific location of the stage  302  to allow the system  320  to precisely deposit a fluid or media at specific locations on the substrate  314  of the substrate nest  312 . 
     In addition, the alignment features  306  may align a plurality of different substrate nests (e.g., substrate nest  312 , etc.) to be utilized with the same stage  302  of the system  320 . For example, system  320 - 1  may illustrate a dispenser device utilizing a dish or Petri dish substrate nest type that can couple a number of Petri dish substrates, system  320 - 2  may illustrate a dispenser device utilizing a slide substrate nest type (e.g., microscope slide substrate nest, etc.) to couple a number of slide substrates, and system  320 - 3  may illustrate a dispenser device utilizing a paper media substrate nest type to couple media substrates. As described herein, the alignment features  306  may align each of the different substrate nest types such that the location and orientation of a substrate nest  312  and/or the location and orientation of the substrate  314  is aligned with a location where a dispense head or nozzle of the system  320  is depositing a particular quantity of fluid as described herein. 
     In some examples, the system  320  may be a dispenser device as described herein. For example, the system  320  may be utilized to deposit fluid or media on to a substrate  314  of the substrate nest  312  coupled to a stage  302  as described herein. In some examples, the system  320  may include a platform that may act as a base for the system  320 . 
     In some examples, a slot of the platform may connect the dispenser stage  300  to a rail system for moving the dispenser stage  300  across the platform as described herein. In this way, the rail system may move the dispenser stage  300  to specific locations on the platform to receive deposited fluid or media from a dispense head of the system  320  positioned above the dispenser stage  300 . As described herein, a pillar may be coupled to a bridge to extend a dispense head of the system  320  over a position of the platform. In some examples, the pillar may be utilized to adjust a height of the bridge and/or adjust the height of the dispense head compared to a level of the platform and/or substrate nest  312 . 
     In some examples, the system  320  may be utilized to deposit a specific quantity of liquid or media at a specific location on the substrate  314  of the substrate nest  312  of the dispenser stage  300 . For example, the substrate nest  312  may be a well plate substrate nest  314  that can couple a well plate substrate  314  that includes a plurality of wells that may hold a particular quantity of liquid. In this example, the plurality of wells of the well plate substrate  314  may be relatively close to other wells. In this example, the height of the dispense head may be adjusted to avoid spillage of liquid or media from a first well of the plurality of wells to a second well of the plurality of wells. 
     In some examples, the dispenser stage  300  can include a latch  316  to couple a substrate  314  to the substrate nest  312 . In some examples, the latch  316  can be utilized to align the substrate  314  with the substrate nest  312 . In some examples, a latch  316  may be utilized to secure a well plate substrate that includes a plurality of wells to a specific position of the substrate nest  312 . In some examples, the latch  316  may be a loaded latch (e.g., spring loaded, etc.) that may release a well plate substrate  314  from the substrate nest  312  by applying pressure on the latch  316 . In some examples, the latch  316  may be coupled to the substrate nest  312 . In some examples, different types of substrate nests (e.g., substrate nest  312 , etc.) may include similar latching devices for securing or positioning particular substrates (e.g., substrate  314 , etc.) to the substrate nest. For example, a dish substrate nest as illustrated in system  320 - 1  may include a latching device that includes a recessed portion to receive a particular dish. In this example, the particular dish may be a Petri dish that may be positioned within the recessed portion. In some examples, the latch  316  can be a substrate holder coupled to the removable substrate nest  312  to align a substrate  314  at a particular location on the removable substrate nest  312 . 
     In these examples, the recessed portion may couple a corresponding dish in a specific location such that the system  320 - 1  may identify a specific location for depositing the liquid or media at the specific location. For example, the specific location of the recessed portion may be a known location or programmed location to a computing device of the system  320 - 1 . In this example, the known location may be utilized for depositing a specific quantity of fluid or media at a specific location within the dish or Petri dish. 
     As described herein, the dispenser stage  300  may be utilized to receive and align a plurality of different substrate nests and/or a plurality of different types of substrate nests. For example, the dispenser stage  300  may be utilized to receive and align a well plate substrate nest to couple a well plate substrate  314  as illustrated on the dispenser stage  300 , a dish substrate nest to couple a dish substrate as illustrated by system  320 - 1 , a microscope slide substrate nest to couple a microscope slide substrate as illustrated by system  320 - 2 , a media substrate nest to couple media substrate as illustrated by system  320 - 3 , and/or other types of substrate nests that may be utilized to couple a substrate to receive the fluid or media from the dispense head of the system  320 . 
     As described herein, the system  320  may determine the type of substrate nest  312  and/or substrate  314  coupled to the dispenser stage  300  and alter a height of the bridge or dispense head based on the type of substrate nest  312  or type of substrate  314  coupled to the substrate nest  312 . In addition, the alignment features  306  may be utilized to align the particular type of substrate nest  312  coupled to the dispenser stage  300  to position the substrate  314  to receive the fluid or media dispensed by the dispense head. 
     In some examples, the dispenser stage  300  may include a base enclosure  330  with a platform (e.g., platform  222  as referenced in  FIG. 2 ), a stage  302  coupled to a rail system to move the stage  302  in a first direction and a second direction across the platform, a retention device (e.g., fastener device or fastener system as described herein) coupled to the stage  302 , a plurality of alignment features  306  coupled to a corresponding plurality of apertures  332 - 1 ,  332 - 2  of the stage  302 , and a removable substrate nest  312  coupled to the retention device and aligned with a microfluidic dispenser dispense head (e.g., dispense head  228  as illustrated in  FIG. 2 , nozzle, plurality of nozzles, etc.) by the plurality of alignment features  306 . 
     In some examples, the system  320  may be a microfluidic dispenser that may include a computing device to deposit a specific quantity of fluid or media at a specific location on a substrate  314  coupled to a substrate nest  312 . In addition, a substrate nest  312  may be non-mechanically removed (e.g., removed without tools, removed without removing mechanical devices such as screws or bolts, etc.) from the stage  302  and replaced with a different substrate nest that is aligned by the alignment features  306  as described herein. Thus, the system  320  may change the type of substrate nest  312  coupled to the stage  302  and align the substrate  314  of the substrate nest  312  coupled to the stage  302  more quickly and easily compared to previous systems and methods that utilize mechanical devices to couple and align a substrate nest  312  with a stage  302 . 
     The above specification, examples and data provide a description of the method and applications, and use of the system and method of the present disclosure. Since many examples may be made without departing from the spirit and scope of the system and method of the present disclosure, this specification merely sets forth some of the many possible example configurations and implementations.